LOCUS       DAPOZO010000001       124814 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPOZO010000001 DAPOZO010000000
VERSION     DAPOZO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 124814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 124814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..124814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.001"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(235..1200)
                     /gene="alx"
                     /locus_tag="ROI21_000001"
     CDS             complement(235..1200)
                     /gene="alx"
                     /locus_tag="ROI21_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDX5108990.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(1484..2470)
                     /locus_tag="ROI21_000002"
     CDS             complement(1484..2470)
                     /locus_tag="ROI21_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDX5108991.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLTVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(2549..3241)
                     /locus_tag="ROI21_000003"
     CDS             complement(2549..3241)
                     /locus_tag="ROI21_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDX5108992.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLTAGATLFIADRVMVNTS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(3318..3821)
                     /locus_tag="ROI21_000004"
     CDS             complement(3318..3821)
                     /locus_tag="ROI21_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDX5108993.1"
                     /translation="MSNLTYLQGYPDQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAISRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            3906..5042
                     /gene="rlmG"
                     /locus_tag="ROI21_000005"
     CDS             3906..5042
                     /gene="rlmG"
                     /locus_tag="ROI21_000005"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDX5108994.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            complement(5160..7178)
                     /gene="fadH"
                     /locus_tag="ROI21_000006"
     CDS             complement(5160..7178)
                     /gene="fadH"
                     /locus_tag="ROI21_000006"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDX5108995.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRNDQWGGDYPNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQSFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGKKVAIIGCGGIGF
                     DTAMFLSQPGESTSKNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(7404..9755)
                     /gene="ygjK"
                     /locus_tag="ROI21_000007"
     CDS             complement(7404..9755)
                     /gene="ygjK"
                     /locus_tag="ROI21_000007"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDX5108996.1"
                     /translation="MKIKTILTPVTCALLISFSAHAADADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAQRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFQHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(9772..10842)
                     /gene="ygjJ"
                     /locus_tag="ROI21_000008"
     CDS             complement(9772..10842)
                     /gene="ygjJ"
                     /locus_tag="ROI21_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDX5108997.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEVRPAEHDDTKTPAITSMS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(10976..12409)
                     /locus_tag="ROI21_000009"
     CDS             complement(10976..12409)
                     /locus_tag="ROI21_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDX5108998.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(12472..12921)
                     /locus_tag="ROI21_000010"
     CDS             complement(12472..12921)
                     /locus_tag="ROI21_000010"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDX5108999.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPSVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(12918..16010)
                     /gene="ebgA"
                     /locus_tag="ROI21_000011"
     CDS             complement(12918..16010)
                     /gene="ebgA"
                     /locus_tag="ROI21_000011"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDX5109000.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(16194..17177)
                     /gene="ebgR"
                     /locus_tag="ROI21_000012"
     CDS             complement(16194..17177)
                     /gene="ebgR"
                     /locus_tag="ROI21_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDX5109001.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            17396..17728
                     /locus_tag="ROI21_000013"
     CDS             17396..17728
                     /locus_tag="ROI21_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDX5109002.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            complement(17770..19149)
                     /gene="ygjG"
                     /locus_tag="ROI21_000014"
     CDS             complement(17770..19149)
                     /gene="ygjG"
                     /locus_tag="ROI21_000014"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDX5109003.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALSECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            19567..21087
                     /gene="aer"
                     /locus_tag="ROI21_000015"
     CDS             19567..21087
                     /gene="aer"
                     /locus_tag="ROI21_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDX5109004.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAVMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(21194..21817)
                     /gene="nfeR"
                     /locus_tag="ROI21_000016"
                     /gene_synonym="yqjI"
     CDS             complement(21194..21817)
                     /gene="nfeR"
                     /locus_tag="ROI21_000016"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDX5109005.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            22094..22858
                     /gene="nfeF"
                     /locus_tag="ROI21_000017"
     CDS             22094..22858
                     /gene="nfeF"
                     /locus_tag="ROI21_000017"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDX5109006.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRAERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQSDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDHACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRHFEAEQYDPQRVRAAAYWHAK"
     gene            complement(22912..22987)
                     /locus_tag="ROI21_000018"
     tRNA            complement(22912..22987)
                     /locus_tag="ROI21_000018"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22952..22954),aa:Ile,seq:cat)
     gene            23112..23618
                     /gene="mug"
                     /locus_tag="ROI21_000019"
     CDS             23112..23618
                     /gene="mug"
                     /locus_tag="ROI21_000019"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDX5109007.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(23697..25538)
                     /gene="rpoD"
                     /locus_tag="ROI21_000020"
     CDS             complement(23697..25538)
                     /gene="rpoD"
                     /locus_tag="ROI21_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDX5109008.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(25733..27478)
                     /gene="dnaG"
                     /locus_tag="ROI21_000021"
     CDS             complement(25733..27478)
                     /gene="dnaG"
                     /locus_tag="ROI21_000021"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDX5109009.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKQAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(27589..27804)
                     /gene="rpsU"
                     /locus_tag="ROI21_000022"
     CDS             complement(27589..27804)
                     /gene="rpsU"
                     /locus_tag="ROI21_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDX5109010.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            28042..29055
                     /gene="tsaD"
                     /locus_tag="ROI21_000023"
     CDS             28042..29055
                     /gene="tsaD"
                     /locus_tag="ROI21_000023"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDX5109011.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(29098..30561)
                     /gene="ttdT"
                     /locus_tag="ROI21_000024"
     CDS             complement(29098..30561)
                     /gene="ttdT"
                     /locus_tag="ROI21_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDX5109012.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLNGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPMVLL"
     gene            complement(30610..31215)
                     /gene="ttdB"
                     /locus_tag="ROI21_000025"
     CDS             complement(30610..31215)
                     /gene="ttdB"
                     /locus_tag="ROI21_000025"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDX5109013.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(31212..32123)
                     /gene="ttdA"
                     /locus_tag="ROI21_000026"
     CDS             complement(31212..32123)
                     /gene="ttdA"
                     /locus_tag="ROI21_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDX5109014.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTDKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            32330..33262
                     /gene="ttdR"
                     /locus_tag="ROI21_000027"
                     /gene_synonym="dan"
     CDS             32330..33262
                     /gene="ttdR"
                     /locus_tag="ROI21_000027"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDX5109015.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQIM"
     gene            complement(33275..33892)
                     /gene="plsY"
                     /locus_tag="ROI21_000028"
     CDS             complement(33275..33892)
                     /gene="plsY"
                     /locus_tag="ROI21_000028"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDX5109016.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            33997..34365
                     /gene="folB"
                     /locus_tag="ROI21_000029"
     CDS             33997..34365
                     /gene="folB"
                     /locus_tag="ROI21_000029"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDX5109017.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            34456..35277
                     /gene="bacA"
                     /locus_tag="ROI21_000030"
     CDS             34456..35277
                     /gene="bacA"
                     /locus_tag="ROI21_000030"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDX5109018.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(35358..36596)
                     /gene="cca"
                     /locus_tag="ROI21_000031"
     CDS             complement(35358..36596)
                     /gene="cca"
                     /locus_tag="ROI21_000031"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDX5109019.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(36660..37280)
                     /locus_tag="ROI21_000032"
     CDS             complement(36660..37280)
                     /locus_tag="ROI21_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDX5109020.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            37522..38823
                     /gene="ygiF"
                     /locus_tag="ROI21_000033"
     CDS             37522..38823
                     /gene="ygiF"
                     /locus_tag="ROI21_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDX5109021.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            38846..41686
                     /gene="glnE"
                     /locus_tag="ROI21_000034"
     CDS             38846..41686
                     /gene="glnE"
                     /locus_tag="ROI21_000034"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDX5109022.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALCNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            41734..43167
                     /gene="hldE"
                     /locus_tag="ROI21_000035"
     CDS             41734..43167
                     /gene="hldE"
                     /locus_tag="ROI21_000035"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDX5109023.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(43287..43346)
                     /gene="ibsE"
                     /locus_tag="ROI21_000036"
     CDS             complement(43287..43346)
                     /gene="ibsE"
                     /locus_tag="ROI21_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDX5109024.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(43662..43721)
                     /locus_tag="ROI21_000037"
     CDS             complement(43662..43721)
                     /locus_tag="ROI21_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDX5109025.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(43962..45623)
                     /locus_tag="ROI21_000038"
     CDS             complement(43962..45623)
                     /locus_tag="ROI21_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDX5109026.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVEEKAE"
     gene            complement(45650..46279)
                     /locus_tag="ROI21_000039"
     CDS             complement(45650..46279)
                     /locus_tag="ROI21_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDX5109027.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            46548..46748
                     /gene="glgS"
                     /locus_tag="ROI21_000040"
     CDS             46548..46748
                     /gene="glgS"
                     /locus_tag="ROI21_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDX5109028.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(46791..47855)
                     /locus_tag="ROI21_000041"
     CDS             complement(46791..47855)
                     /locus_tag="ROI21_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5109029.1"
                     /translation="MRYLLIVITFFMGFSSLPAWAMDCYAEHEGGNTVVIGYVPRISI
                     PSDGKKGDKIWQSSEYFMNVFCDNALPGPSPGEESPSAWANIMMLLASGQDFYNQNSY
                     TFGVTYNGVDYDSTSPLPIAAPVCIDIKGAGTFGNGYKKPAVCSGGPEPQLSVTFPVR
                     VQLYIKLAKNANKVNKKLVLPDEYITLEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVDLEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDIGNGLLLKIFHNDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(47857..48606)
                     /locus_tag="ROI21_000042"
     CDS             complement(47857..48606)
                     /locus_tag="ROI21_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDX5109030.1"
                     /translation="MRYLNTKNIIAAGVLLSCMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMITQEKIAQL
                     PKDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMTYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGVTMLPQTQSLMVTPFSYANTQFKNVPSTFQVG
                     YINDFGGLSFYEINCPVVNNICNISVANRDQ"
     gene            complement(48622..51126)
                     /locus_tag="ROI21_000043"
     CDS             complement(48622..51126)
                     /locus_tag="ROI21_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX5109031.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVIIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLSTLINNKRTPIHLDLQWVLIDNQTAVCVTPEQLTL
                     LGFTDEFIEKTQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWNHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQATNLDFPRIYLFRPIPAMNAKLTIGQYDTESSIFDSFHFSGISLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGRVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNEISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GTEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIIGVIRLADGSHPPLGISVKDETSHKELGLVADGGFVYLNGIQD
                     DNKLALRWGDKSCFIQPPNSSNLTTGTAILPCISQN"
     gene            complement(51186..51737)
                     /locus_tag="ROI21_000044"
     CDS             complement(51186..51737)
                     /locus_tag="ROI21_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDX5109032.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQSDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            complement(52021..52311)
                     /gene="ubiK"
                     /locus_tag="ROI21_000045"
     CDS             complement(52021..52311)
                     /gene="ubiK"
                     /locus_tag="ROI21_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDX5109033.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
     regulatory      52433..52581
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            52685..53338
                     /gene="ribB"
                     /locus_tag="ROI21_000046"
     CDS             52685..53338
                     /gene="ribB"
                     /locus_tag="ROI21_000046"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDX5109034.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            53600..53770
                     /locus_tag="ROI21_000047"
     CDS             53600..53770
                     /locus_tag="ROI21_000047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109035.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(53828..54601)
                     /gene="zupT"
                     /locus_tag="ROI21_000048"
     CDS             complement(53828..54601)
                     /gene="zupT"
                     /locus_tag="ROI21_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDX5109036.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            54744..55532
                     /gene="ygiD"
                     /locus_tag="ROI21_000049"
     CDS             54744..55532
                     /gene="ygiD"
                     /locus_tag="ROI21_000049"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDX5109037.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVEGIEMGSLSMLSVQIG"
     gene            complement(55570..56730)
                     /locus_tag="ROI21_000050"
     CDS             complement(55570..56730)
                     /locus_tag="ROI21_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDX5109038.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(56736..57407)
                     /locus_tag="ROI21_000051"
     CDS             complement(56736..57407)
                     /locus_tag="ROI21_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDX5109039.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(57555..59036)
                     /gene="tolC"
                     /locus_tag="ROI21_000052"
     CDS             complement(57555..59036)
                     /gene="tolC"
                     /locus_tag="ROI21_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDX5109040.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            59241..59870
                     /gene="nudF"
                     /locus_tag="ROI21_000053"
     CDS             59241..59870
                     /gene="nudF"
                     /locus_tag="ROI21_000053"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDX5109041.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            59871..60293
                     /locus_tag="ROI21_000054"
     CDS             59871..60293
                     /locus_tag="ROI21_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDX5109042.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            60318..61145
                     /gene="cpdA"
                     /locus_tag="ROI21_000055"
     CDS             60318..61145
                     /gene="cpdA"
                     /locus_tag="ROI21_000055"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDX5109043.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            61145..61726
                     /gene="yqiA"
                     /locus_tag="ROI21_000056"
     CDS             61145..61726
                     /gene="yqiA"
                     /locus_tag="ROI21_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDX5109044.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            61755..63647
                     /gene="parE"
                     /locus_tag="ROI21_000057"
     CDS             61755..63647
                     /gene="parE"
                     /locus_tag="ROI21_000057"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDX5109045.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(63695..64009)
                     /locus_tag="ROI21_000058"
     CDS             complement(63695..64009)
                     /locus_tag="ROI21_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDX5109046.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(64040..64621)
                     /gene="mdaB"
                     /locus_tag="ROI21_000059"
     CDS             complement(64040..64621)
                     /gene="mdaB"
                     /locus_tag="ROI21_000059"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDX5109047.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            64940..65272
                     /locus_tag="ROI21_000060"
     CDS             64940..65272
                     /locus_tag="ROI21_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HDX5109048.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIVLILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            complement(65318..66667)
                     /gene="qseC"
                     /locus_tag="ROI21_000061"
     CDS             complement(65318..66667)
                     /gene="qseC"
                     /locus_tag="ROI21_000061"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDX5109049.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNRLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(66664..67323)
                     /gene="qseB"
                     /locus_tag="ROI21_000062"
     CDS             complement(66664..67323)
                     /gene="qseB"
                     /locus_tag="ROI21_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDX5109050.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            67475..67867
                     /gene="ygiW"
                     /locus_tag="ROI21_000063"
     CDS             67475..67867
                     /gene="ygiW"
                     /locus_tag="ROI21_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDX5109051.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            67920..68402
                     /locus_tag="ROI21_000064"
     CDS             67920..68402
                     /locus_tag="ROI21_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDX5109052.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            68607..68903
                     /gene="mqsR"
                     /locus_tag="ROI21_000065"
     CDS             68607..68903
                     /gene="mqsR"
                     /locus_tag="ROI21_000065"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HDX5109053.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            68905..69300
                     /gene="mqsA"
                     /locus_tag="ROI21_000066"
     CDS             68905..69300
                     /gene="mqsA"
                     /locus_tag="ROI21_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HDX5109054.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            69433..71040
                     /locus_tag="ROI21_000067"
     CDS             69433..71040
                     /locus_tag="ROI21_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDX5109055.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            71178..73436
                     /gene="parC"
                     /locus_tag="ROI21_000068"
     CDS             71178..73436
                     /gene="parC"
                     /locus_tag="ROI21_000068"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDX5109056.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            73670..74407
                     /gene="plsC"
                     /locus_tag="ROI21_000069"
                     /gene_synonym="parF"
     CDS             73670..74407
                     /gene="plsC"
                     /locus_tag="ROI21_000069"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDX5109057.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPTDAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            74482..75894
                     /gene="ftsP"
                     /locus_tag="ROI21_000070"
     CDS             74482..75894
                     /gene="ftsP"
                     /locus_tag="ROI21_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDX5109058.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMNDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            76005..78224
                     /locus_tag="ROI21_000071"
     CDS             76005..78224
                     /locus_tag="ROI21_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDX5109059.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(78267..78524)
                     /gene="yqhH"
                     /locus_tag="ROI21_000072"
     CDS             complement(78267..78524)
                     /gene="yqhH"
                     /locus_tag="ROI21_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDX5109060.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(78575..79501)
                     /locus_tag="ROI21_000073"
     CDS             complement(78575..79501)
                     /locus_tag="ROI21_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDX5109061.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATASY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            complement(79701..80528)
                     /gene="dkgA"
                     /locus_tag="ROI21_000074"
     CDS             complement(79701..80528)
                     /gene="dkgA"
                     /locus_tag="ROI21_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDX5109062.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            complement(80633..81796)
                     /gene="yqhD"
                     /locus_tag="ROI21_000075"
     CDS             complement(80633..81796)
                     /gene="yqhD"
                     /locus_tag="ROI21_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDX5109063.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            81990..82889
                     /gene="yqhC"
                     /locus_tag="ROI21_000076"
     CDS             81990..82889
                     /gene="yqhC"
                     /locus_tag="ROI21_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDX5109064.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            complement(82886..83587)
                     /locus_tag="ROI21_000077"
     CDS             complement(82886..83587)
                     /locus_tag="ROI21_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDX5109065.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKNTVT
                     PDDFPRSRLLYRERI"
     gene            complement(83727..84914)
                     /gene="metC"
                     /locus_tag="ROI21_000078"
     CDS             complement(83727..84914)
                     /gene="metC"
                     /locus_tag="ROI21_000078"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDX5109066.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            85166..85900
                     /gene="exbB"
                     /locus_tag="ROI21_000079"
     CDS             85166..85900
                     /gene="exbB"
                     /locus_tag="ROI21_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDX5109067.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            85907..86332
                     /gene="exbD"
                     /locus_tag="ROI21_000080"
     CDS             85907..86332
                     /gene="exbD"
                     /locus_tag="ROI21_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDX5109068.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(86604..87488)
                     /gene="yghA"
                     /locus_tag="ROI21_000081"
     CDS             complement(86604..87488)
                     /gene="yghA"
                     /locus_tag="ROI21_000081"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDX5109069.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHKAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            87679..88173
                     /locus_tag="ROI21_000082"
     CDS             87679..88173
                     /locus_tag="ROI21_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDX5109070.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            complement(88213..89253)
                     /gene="gpr"
                     /locus_tag="ROI21_000083"
     CDS             complement(88213..89253)
                     /gene="gpr"
                     /locus_tag="ROI21_000083"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDX5109071.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTKELAQIDQHIAD
                     GELNLWQASSDK"
     gene            89410..90294
                     /locus_tag="ROI21_000084"
                     /pseudo
     CDS             89410..90294
                     /locus_tag="ROI21_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
     gene            90413..90700
                     /locus_tag="ROI21_000085"
     CDS             90413..90700
                     /locus_tag="ROI21_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDX5109072.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            90889..92007
                     /gene="hybO"
                     /locus_tag="ROI21_000086"
     CDS             90889..92007
                     /gene="hybO"
                     /locus_tag="ROI21_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDX5109073.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            92010..92996
                     /gene="hybA"
                     /locus_tag="ROI21_000087"
     CDS             92010..92996
                     /gene="hybA"
                     /locus_tag="ROI21_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDX5109074.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            92986..94164
                     /gene="hybB"
                     /locus_tag="ROI21_000088"
     CDS             92986..94164
                     /gene="hybB"
                     /locus_tag="ROI21_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDX5109075.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            94161..95864
                     /gene="hybC"
                     /locus_tag="ROI21_000089"
     CDS             94161..95864
                     /gene="hybC"
                     /locus_tag="ROI21_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDX5109076.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            95864..96358
                     /locus_tag="ROI21_000090"
     CDS             95864..96358
                     /locus_tag="ROI21_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDX5109077.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            96351..96839
                     /gene="hybE"
                     /locus_tag="ROI21_000091"
     CDS             96351..96839
                     /gene="hybE"
                     /locus_tag="ROI21_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDX5109078.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            96832..97173
                     /gene="hypA"
                     /locus_tag="ROI21_000092"
     CDS             96832..97173
                     /gene="hypA"
                     /locus_tag="ROI21_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDX5109079.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            97186..97434
                     /gene="hybG"
                     /locus_tag="ROI21_000093"
     CDS             97186..97434
                     /gene="hybG"
                     /locus_tag="ROI21_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDX5109080.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(97557..98423)
                     /gene="yghU"
                     /locus_tag="ROI21_000094"
     CDS             complement(97557..98423)
                     /gene="yghU"
                     /locus_tag="ROI21_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDX5109081.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            98628..100487
                     /gene="gss"
                     /locus_tag="ROI21_000095"
     CDS             98628..100487
                     /gene="gss"
                     /locus_tag="ROI21_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDX5109082.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            100779..102278
                     /gene="pitB"
                     /locus_tag="ROI21_000096"
     CDS             100779..102278
                     /gene="pitB"
                     /locus_tag="ROI21_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDX5109083.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVAAVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(102327..103019)
                     /locus_tag="ROI21_000097"
     CDS             complement(102327..103019)
                     /locus_tag="ROI21_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDX5109084.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            103193..103906
                     /locus_tag="ROI21_000098"
     CDS             103193..103906
                     /locus_tag="ROI21_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDX5109085.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASQRAIHAALS"
     gene            103938..104696
                     /locus_tag="ROI21_000099"
     CDS             103938..104696
                     /locus_tag="ROI21_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109086.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            104742..106091
                     /locus_tag="ROI21_000100"
     CDS             104742..106091
                     /locus_tag="ROI21_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDX5109087.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVFSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            106091..106915
                     /locus_tag="ROI21_000101"
     CDS             106091..106915
                     /locus_tag="ROI21_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109088.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLKFWLDGVNRQELLRLIRKQAKGDELTTDERKKYKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPL"
     gene            106927..107487
                     /locus_tag="ROI21_000102"
     CDS             106927..107487
                     /locus_tag="ROI21_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDX5109089.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKYGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            107518..108588
                     /gene="lptF"
                     /locus_tag="ROI21_000103"
     CDS             107518..108588
                     /gene="lptF"
                     /locus_tag="ROI21_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDX5109090.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            108585..109664
                     /gene="lptG"
                     /locus_tag="ROI21_000104"
     CDS             108585..109664
                     /gene="lptG"
                     /locus_tag="ROI21_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDX5109091.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLALLFALSAP
                     VTALGLPIVFVLVALSLVYWYDSQH"
     gene            complement(109699..110871)
                     /locus_tag="ROI21_000105"
     CDS             complement(109699..110871)
                     /locus_tag="ROI21_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDX5109092.1"
                     /translation="MGLYDKFARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRSGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(110871..111119)
                     /locus_tag="ROI21_000106"
     CDS             complement(110871..111119)
                     /locus_tag="ROI21_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDX5109093.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(111151..112065)
                     /locus_tag="ROI21_000107"
     CDS             complement(111151..112065)
                     /locus_tag="ROI21_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDX5109094.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     IWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAADARCGSVRMVGIPLSVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SANNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(112062..113795)
                     /locus_tag="ROI21_000108"
     CDS             complement(112062..113795)
                     /locus_tag="ROI21_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDX5109095.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFSCQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAW
                     FKALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRP
                     GDRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSV
                     APPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTF
                     MPCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPDAETRAVSTFVNCGKAL
                     PEHGIEIRNEAGIPVAERVVGHICISGPSLMSGYFGDQISQDEIAATGWLDTGDLGYL
                     LDGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQC
                     RISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAY
                     AASLHVQESLA"
     gene            114158..115300
                     /locus_tag="ROI21_000109"
     CDS             114158..115300
                     /locus_tag="ROI21_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDX5109096.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQQNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(115307..116071)
                     /gene="glcC"
                     /locus_tag="ROI21_000110"
     CDS             complement(115307..116071)
                     /gene="glcC"
                     /locus_tag="ROI21_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDX5109097.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNQS"
     gene            116322..117821
                     /gene="glcD"
                     /locus_tag="ROI21_000111"
     CDS             116322..117821
                     /gene="glcD"
                     /locus_tag="ROI21_000111"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDX5109098.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            117821..118873
                     /gene="glcE"
                     /locus_tag="ROI21_000112"
     CDS             117821..118873
                     /gene="glcE"
                     /locus_tag="ROI21_000112"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDX5109099.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLSCEPPHYGEEATWGGMI
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQKLREQQLPFFSLPGTLWRISLPCDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            118884..120107
                     /gene="glcF"
                     /locus_tag="ROI21_000113"
     CDS             118884..120107
                     /gene="glcF"
                     /locus_tag="ROI21_000113"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDX5109100.1"
                     /translation="MQTRLTEDMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLRKEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALDKE"
     gene            120112..120516
                     /locus_tag="ROI21_000114"
     CDS             120112..120516
                     /locus_tag="ROI21_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDX5109101.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            120538..122709
                     /gene="glcB"
                     /locus_tag="ROI21_000115"
     CDS             120538..122709
                     /gene="glcB"
                     /locus_tag="ROI21_000115"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDX5109102.1"
                     /translation="MSETITQGLLRIDADFKRFVDEEVLPGVELDAAAFWRNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLCELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFIG
                     YRGNAAAPSCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTSAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            123064..124746
                     /gene="glcA"
                     /locus_tag="ROI21_000116"
     CDS             123064..124746
                     /gene="glcA"
                     /locus_tag="ROI21_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDX5109103.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     TTLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSACAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT        29673 a        31185 c        33034 g        30922 t
ORIGIN      
        1 cctcagctta tcccttgcgg ctaccgggtc gggcgcaagg gaacgtccct gtaagatgcg
       61 ccctcgccgc gcatccatgc gcgtcgcccc ggcctccagg gaacgcttcg gcgattttga
      121 cgccaccaac acccgtgcgg tctttttgta aagagctata agccgattac ataattaata
      181 gctcaccttt gtctggaatg agttatctct agtaatggct taatatacag cgaatcattc
      241 tccccgctgc ttatcatgcc gataattcac ccaggcgttg ataataaacg tcatcaccag
      301 aatgccaaac accacgccca gcgagacggc gattggaata tggtagaagt cgacaatcag
      361 catcttgata ccgataaaca ccagaatcac cgccaggcca tatttgagca tcgagaaacg
      421 ttctgctacg cccgccagca ggaaatacat cgcacgcagg ccgaggatcg caaacaggtt
      481 tgaggtcagc acaatgaacg ggtcagtggt cacggcgaag atagccggaa tgctatccac
      541 ggcgaaaatc acgtcgctca attccaccag aatcagcacc agcatcagcg gcgtggcata
      601 cagcaagcca tttttacgca caaagaaatg ctcgttgtcg atggtgtcgg tcatacgcaa
      661 atgaccgcgc agccagcgca ccagcggctt gtcgccaatg cctgattcat cttcatgggc
      721 cagcgccatc ttcacgccgg taaacagcag gaaggcaccg aagatataca ggatccagtc
      781 gaactgcgaa atcagccagc tgccagtgaa gatcatgatg gtacgcagaa caatcgcccc
      841 gagcacgcca tacaccagca cgcggcgttg taatgccgcc ggaacagaga aatagctgaa
      901 caacatcagc cagacaaaga cgttatcgac cgccagcgat ttctcaatca gataacccgt
      961 gagaaaggcc agtgcctgtg gatcggcaac ggcgcgacct tcggtttgca ccagatacca
     1021 ccagaaagcg gcgttaaaca gtaacgacag cgtcacccag accagcgacc aggccgcagc
     1081 ctgtttcatg gtcatggcat gtgccccacg acgcccctgc aacaacaggt cgatagccag
     1141 cataatggcg acaacaacag cgaatccgcc ccatagcaac ggcgtgccga cagtattcat
     1201 agaagttcct tacacataaa aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt
     1261 atgcatagac ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa tacgtctgcg
     1321 tattcctttc gggttgcccg gtgaccggat gtggtttttc acacatcgta atgacgatcg
     1381 accggcaatg aagttactcc cctttgcagg taacaaagta tgacagacca ttcagccagt
     1441 caatggtctg ttacatacct tttacactgt tttactttgg caattatagt tttacgctat
     1501 ctgctggaaa aatcacccca gtctggcggc ggatctcggt cagcagtttg gcggtgatgc
     1561 gactgaccgt cagccctgga tgatccacca gatgctcatc caccagttca gcaaacagct
     1621 ctgcttcgta gagcatggta ttaatatgct gcggctgggt gagatcctgc atctggctgc
     1681 cacgcggcac aaagcagact ttctggcatt cagacagttt ttcaatcacc agcgatcctg
     1741 cttcgccctg aatctcgctt gccaggacag aatcactcac ttttgagtgc tgcaaggtga
     1801 cgctgaaatc accgtaatcc atcaccacca cgccgtgggc atctacgccg cttgccaaca
     1861 aactggcggt tgcctgcacg cttttcggtt caccaaataa cgctaccgcc gacgccaggc
     1921 agtaaaagcc gatatccata atcgaaccgt tagagaatgc tggattaaag gtgttgggat
     1981 tctcaccgtc caggtaacgt tgataccgcg aggaatactg gcaatagttg aaaaagactt
     2041 tacgcagttt gccgactttc ggcagtgcct ggcgtaacag atgaaagttc ggcaggtagg
     2101 ccgttttaaa tgcctcaaac agcaccacct gattttcccg cgcacaggca atggcggcat
     2161 ccacttccgc cagattcgat gccagcggct tctcgcaaat cacatgaatt ttgtggctaa
     2221 ggaaaagttg tgtctgggaa aagtgcaggg cattcgggct ggcaatatac accgcgtcaa
     2281 tggcatcgct ttccgccatc gcttccagcg acgtaaacag atgctcgaca gaaaaatcat
     2341 tggcaaagtg ctgggcctgt tcaaggctgc gggaatatac ggcggttaac ttgtatttac
     2401 cgctctcatg ggcggcctcg acgaactggc gagtgatcca gttcgtacca atcacagcga
     2461 aacgtatcat aacgcgtgga tactccataa agggattctg tcgtcaatgt agcacgcctt
     2521 tttcatcttc gacgtgcgca gatacgcatt attctttcgc cggacagcca tgctcactaa
     2581 acggcccaat catgaccgat ggcccgagat atttcggctg gcgatggagg atttttgcgt
     2641 caatcaccgc gctgactctg gcgagtttct cccgtaactg agtataaaaa ccgtgcttca
     2701 tattgaggtc tggcgcgtta aagccgatag cgtcgatgcc gtactgtttt gccagccaga
     2761 tggcgcgttg gttatgaaat tcttgcgaaa taatggtgat atggttttcc ccaaacacct
     2821 ttttggcacg cactaccgaa tccagcgttg agaatccggc atagtcgcag aagatcactt
     2881 tcgccggtac gcctttagcg atcaaagcct gctgcatacc tgaggcttca tcgtaatttt
     2941 tacgtccgtt atcgccactt accagtagcc atttcacttt tccggcgtgg tacaacgctg
     3001 ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg cctcgccccc agcaataaac
     3061 ccacgttacg cgccggaact gcgttgacat cgctccaggt cagctgcttg ctggtgttga
     3121 ccataacgcg atcggcaata aaaagggtcg ctcctgctgt aagcaacaat aaacagccaa
     3181 tcttaagagt ccggcgagaa aaacaaatac ggagaagaag gcgggcaaag gcgcgcagca
     3241 tggtcgttat ccttaacgaa aggggggagg tcgattttag gcgctaaagg aagaaaagat
     3301 aacgcctgac atcattttca gattttgtcc tgcccaagtg acaactgcgt tagccacaga
     3361 cgagtgtcga attcaagctg gtggtactgc ggctccatat gacagcacaa ttgatagaac
     3421 gctttgttat gctctttctc cttcaggtgc gccaactcgt gcaccacgat catgcgcaaa
     3481 aacggttccg gcgcgttgcg aaacacggta gcgacgcgga tctccgcctt tgctttcagc
     3541 ttaccgccct gcacacgcga aatagcggta tgtaacccga gcgcgttttt cagcacatgg
     3601 atcttattgt catacatcac tttagtgatc ggcggggcat tgcgcagaaa ctgatttttc
     3661 agatcctggg tatattgcca gagggcttta tcggtggcgt agtcgtgtgt tcccggatag
     3721 cgttttgcca gcacatcgcc cagacgctgt tcgttaatca gcgttcgcac ctgggaaagt
     3781 agctggtcgg gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag
     3841 aaaaagggta tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag
     3901 ggccgatgag ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga
     3961 cggatgacgt taacccgcta caggcgtggg aagcggcgga tgaatatttg ctgcaacagt
     4021 tggacgacac agaaatccgc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa
     4081 gttgtgcact ggcggaacat aagccttaca gcattggcga ctcatacatc agcgaactgg
     4141 cgacgcgcga gaatttacgc ctcaacggga ttgatgaatc gagcgtgaag tttctcgaca
     4201 gcaccgctga ctacccgcaa cagccgggcg tggtactgat caaagtaccg aaaacactgg
     4261 cgttgctgga acaacaactg cgtgcgctgc gcaaagtggt cacgccggat acacgtatta
     4321 ttgccggtgc caaagcccgt gacatccaca cttccacgct ggaactgttc gaaaaagtgc
     4381 tcggcccgac caccaccacg ctggcatgga agaaggcacg cttaattaac tgtactttca
     4441 acgagccgcc gctggccgat gcgccgcaga ccgttagctg gaaactggaa ggtactgact
     4501 ggactatcca caaccatgcg aatgtcttct cccgcaccgg gctggatatt ggcgcgcgct
     4561 tctttatgca gcatctgcca gagaatctcg aaggtgagat tgtcgatctc ggttgtggta
     4621 atggcgttat tggtctgacg ctgcttgata aaaacccgca ggcgaaagtg gtgtttgtcg
     4681 atgaatcgcc gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgccagagg
     4741 cgttggatcg ctgcgagttt atgatcaaca acgcgctctc cggcgtggag cctttccgct
     4801 ttaatgctgt gctctgcaac ccgccgtttc atcagcaaca tgcgctgacc gataacgtcg
     4861 cctgggagat gttccaccac gcccgccgct gtctgaaaat caacggcgag ctgtatatcg
     4921 ttgccaaccg tcacctggat tacttccata aactgaagaa gattttcggc aactgcacca
     4981 ccatcgccac gaataataaa tttgtggtgc tgaaagcagt gaagctgggg cgtcgtcggt
     5041 aagattggca gctacctgcc agataaggcg taaacgcctt atctggcata cgttcagcac
     5101 attaccccca ggtcggataa gacgcggcaa gcgtcgcatc cggcacttca atgcacgatt
     5161 taaatctcca gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata
     5221 gctacatcgc agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc
     5281 agcgcgcggt ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc
     5341 gtttcgccgt tgatcaccac atgcagcccg tcatcgtcga tcttctgata acttacgcct
     5401 gggatcattt tcacgccgcg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg
     5461 cctaacccct gtcccggttt gctggctttg cgctggagca tcacaatctg ccgtgggcta
     5521 cgcgggatct gcattccctg cgggcttaag ccaccagcct gttgcaggct gctgtcgatc
     5581 ccccactcat tacagaactc ggcaatgttc ttgctggtgg actcacccgg ctgacttaaa
     5641 aacatcgccg tatcaaaacc aatcccgcca caaccgatga ttgctacctt tttgcccaca
     5701 ggcgctttgt cgcgcagtac gtcgagataa ctcaacacct tcggatgatc gattccgtcg
     5761 ataggtggaa tgcgcggtac gatcccgcta gcgaggatcg tttcatcgaa cgactgtaac
     5821 tgatccgccg tcacggtgtg attgagtttc agcgtcacgc ctgttacttc gatcatccgg
     5881 cggtaatagc gcagcgtttc gtaaaactcc tctttgccag ggatctgttt ggcgatatta
     5941 aactgaccgc cgatctcact gtgagcatca aacaacgtta cctgatgacc acgtgccgcc
     6001 gcgttaatgg caaacgccag cccagccgga ccagcgccga ccaccgccag attttttttc
     6061 tgcacggcgg gaaggatcgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac
     6121 gaggtgactt tgccaacgaa gatctgatcg aggcaggctt gattgcagcc aatacaggtg
     6181 ttgatctcat cggctcgccc cgattgcgct tttgacagca gttccgcatc agcaagaaac
     6241 ggtcgcgcca tcgataccat atcggcatca ccgcgcgaga gaatgtcgtc ggcaacctgc
     6301 ggatcgttaa tccggttggt ggtcaccagc ggtagcgaga cgtggccttt cagtttgcgc
     6361 gtgacccagc taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat acgcgcttca
     6421 tgccagccaa tgccagtatt aataatggtt gcgcccgccg cttcgatggc ctgcgccagt
     6481 tctaccgttt cggcaaaagt accgccgcct tcgaccaggt cgagcatcga cagacggtag
     6541 ataataatga agtcattgcc gacgcgttcg cgcactgctc gcaccacttc aactgcaaag
     6601 cgcatccggt tagggtaatc gccgccccac tggtcgttac gctggttagt gcgcagggtc
     6661 agaaattcgt tgatcaaata cccttcggaa cccatcacct ctacgccatc gtatccggcc
     6721 tcccgcgcca gttgcgcgca gcgggcgaaa ttgtcgatca gttgcaggat ctcttcatgg
     6781 cttaactcat ggggcacgaa acggttgatg ggagcctgca atgcggaagg agcgaccaga
     6841 tgcggttggt agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc
     6901 tgatgtacgg cttcggttat ggtgcgatgg tgcgggatct ggcttgcgtc attgagcatt
     6961 gcgccgcctt ccatgccaac gcctgttaaa tctggtgcaa taccgccgct gacaatcaac
     7021 gccacgccat gacgggcgcg ttccgcgtaa aacgctgcca gccgttcagc gccgtccggg
     7081 tattcctcca gtcccgtgtg cattgaaccc atcagtactc ggttttttaa cgtggtgaag
     7141 cctaaatcca gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat
     7201 aattgttatg tggtcggatg agttctaatt tagccgtcgc cggagaaaag ggaaaagggg
     7261 agtgcggggt ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcaac
     7321 gcttgccgcg ttttatccgg cctgggggat tgcacatgta gggcggataa ggcgtttacg
     7381 ccgcatccgc cagccattgc aaattactgc ttacggaaaa aatcgttata caacatatac
     7441 aaatgcgcgg cactccagga gaaattgggt gctccttgct gcgcgccagt gagcgggttg
     7501 taattctcct gaatcggacc atcggcggtt aatcctttgg cgtgctggaa gaacgtatcc
     7561 gccagcttca gggcatcatc gcgataaccg taacgctcca tccctttcag accaaaccag
     7621 aactgatcca cccacacgcg cccgcgccag taaatatctg cgccaaaggc cggattggtt
     7681 aacgccgccg ttcccagcgg aacaaaggtg ttgaactctt taggatcgag catcaccttc
     7741 accaccgcgt cggcgttggc ctgcgttgct gcaccgttaa acagcggtga ccagccttct
     7801 ggccctttac cgcgctcgac aatcggtttg cccgcgcagc cgttcgccag tggtttatct
     7861 tcaatgcgca cgtcatagta gaattgtgta gtcgggtcga acatacaggt gttgatgtag
     7921 tccgcgagct gctgtgccaa ctggcgatag cgttgggcct cttccggctt accgagtatg
     7981 gttgccatct ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac
     8041 tcctgcaata gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc
     8101 accgtccagt cgctacgttt gccgccatta gcgacatatt tatccagctg ttctttgtcg
     8161 ataaacccaa agacggcggc gtcatcacga cctgattccc acgacgcagc aacctgtgcc
     8221 ggaatttcca gactgtcgta ctggcctttc tccaccacac gggcgtagtt gttcagccca
     8281 gactgcgtct cttctttgtc gccttttttc accgtaaaca gcatctcgcc gctctcggtg
     8341 ttgtgggctt tgtcgcgggt cgcgccatat tccggcacgc cgttgccgtt gtgatcgcgg
     8401 ttacgtaacc accagtcgtg ataggccacc agcttcgggt acatctctgc caaccaggct
     8461 ttatcctggg tgacgttgta cacttccatc accgaccagg cggcaaggct gggtttggta
     8521 ttacgttcgt tccagttgcc gccatcgccg ccacgctcgg ggctaaggtt ccacgctatc
     8581 aggtcgggaa caaagcccac atcctgcgga cgcacgcggt cgccgggctg gatctgccag
     8641 gagaagaccg cgcggatatt ctctttggcg atgtccggat tgaaatgcgc catcgcaaac
     8701 gcctgcttcc aggtatccca cggccaggtc tgattgccgg agaaccagcg cccagtcacc
     8761 gacggcgtga cggtgttata tttcaccgca ccgcccggtg agcgccagtt accgttgagc
     8821 gtttcgatcg ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac
     8881 cctttcttca gatactcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc
     8941 gccagaatat cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg
     9001 gaataggtgg tgtagagcgt cgtcgaaccg ttgatatgcg ccttactggt aaagcgatta
     9061 ccgttgattt cagtctgcac cggcagggat ttatgcacct gatattctga ttcaccggag
     9121 gtcagcagat cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg
     9181 gcgctgattt tgcgctgata gtcagggtac tcgccagcaa tggttttatc ggaaagcggt
     9241 ttcccttctt tcgcttccag tttttccagc agttcaccgt cccacaccag atccagcggt
     9301 ttattgctgg tgattttggt ttccagtagt gacgtgcgcg gcgtggcgaa gcgaagagtc
     9361 atttcgacct gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat
     9421 gcctccagcg tgaagtcgac tttcttgccg tcctgccaga cggtcaggcg gtcgaaattg
     9481 ctggccataa agttgatgta ttcttccgtc agcagcgcaa cgcctggaaa accgcccatg
     9541 gtattcgggc cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta
     9601 aaacgctggt gatcgtcgta atcgtaatcc ttcatgtact gcggcgcgcc agtacggtta
     9661 atcacgtttt tataattgtc ggcgtcagcg gcatgggcgg aaaaacttat cagcagagcg
     9721 caggttactg gcgttaaaat agtttttatt ttcatgagat tacagtcctt attaccagta
     9781 aaaatattgc atcaggaaga tttggtcgct gggatcgtga gaggcatttt tcatgtagaa
     9841 gcggctatca aaagccgttg cgaaacgtcc gccgccgtat tcgtaccaga taccaaactg
     9901 catgaaagag gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc
     9961 ggtggagaga taaacgccct ttaacgcttc atacatggcg ctcaccatca tcccgctttc
    10021 acttcccggt acgtccagcc cgtcggcgcg tccagtatgg tgccaggcgc gagcggcaaa
    10081 gttaggagta agcaggcccg tgagatagag ctgtcctgtg ccgtcggtga tttccagacc
    10141 gttcatccag gtgatgcctt cggcaaggtc atactggatg taaccgttca tcattgccgg
    10201 ataggtgtat ttgtcgtcgt agcgatcgta cttgccaaaa tgtagccagg ctttgctttc
    10261 gtcatgatgt ccggcaggtg tggcggtaac gctataacga aagttgccgc ttaagttctg
    10321 caccttcagc gcggtcataa tgtcgcgggt attgggaatg acgtagccca gatcgggggt
    10381 gaaatcgccc caccattgca ggtcatccag tgaggaatcc tgacgcagac tgagcatgat
    10441 ttcggtgcca tcattggtac gataaccgcc atagaaacgg ttaattccgc cctcgaaacc
    10501 accccagcta tggtctggca cccaggcatt gccgtcgtgg tcggcctgca cggtccagcc
    10561 ttcgccgtaa atcaggccga accaggatcc gtacttcatc tccaggccac cttcaatata
    10621 ggtgccgtcg ctgtatttat gtttattctc accctctaaa tccatatatc caccaacccc
    10681 taattcgccg taaaaacgaa atgaaggaga agacatcgag gtaatggcgg gcgtttttgt
    10741 gtcgtcatgt tctgccggac gtacttcttc tcctgccgca gaaaaggcgt agcaaaacgg
    10801 cagagcaagt aatgctctgc atggtgcagt gattaatttc attgtgttaa tccatgagct
    10861 aaagaaaaaa tacagccttt tatcgttgta attataaaaa tataaaggca gaaactattt
    10921 tccctctcct catccaggaa gagggaaaat gcctgctcat tttttattag tctgcttaat
    10981 caacattgct ggctggcgtc gcttcaatat ggcgctcttc agccgttaat ccctttatat
    11041 atttactgta tttccaccac gcaaagccga ggaagataac aataccgccg acgttataaa
    11101 aaatgatggt cagaatattc gcgccagtcg ggaatgtcga agcgacaaac cctacggcaa
    11161 atatcgcaat cagcattgaa acaacgataa taccggtgcg gcgggagccc atacggaaat
    11221 cgcgtggcag gtgatctaat ttggcgcgta aattcaggta agccagcatg atgaataacg
    11281 gcggaagcat ggacgctgcg gcggtcatat taataatggt attcatcaga tcctgcacgg
    11341 tattggaacc gagcatcggg ataatcatca gcgggatgac gatcaggaac tggatccacg
    11401 ctgcgcgcgc cggaacgccg ttttcgttca gttcgacggt tttcttacca aagatgcctt
    11461 ccgggatttc ggagaagaaa attttcaccg gcgttgcggt ccacatcagc agggaaccga
    11521 acatcgcggt aaaggacacc agaccgacaa agcgattcat caacgcttcc ggcagaccaa
    11581 aatacgccgc cataccgtgg aatacctgca ccgatccgcc agtaaatttc aactctttgc
    11641 tgctgacgaa gacgttaatc agcaccgagg agacggaata cagtacgccg ataaaaatcc
    11701 cggcgaggat gatcacttta acgaacgatt tcgaaccgcc tttgacgtcg ttaacgtaca
    11761 cagcgacgga ctccgcgcca cctgcggcca taaagatcca ggtggtcacg ccgaggaacg
    11821 cccagttgaa gttcgggatc atcgcgtcaa cggtgatcgg atcggcaggc tgtacgccgc
    11881 caaccagcgc cgtgcctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg
    11941 aagtgacgga ggtgattggc cccagcattt tcgccccgtt ggtggaaacc catgtggaga
    12001 aggcgaacag caccatactg ataatggtgg tagcaaccgg tgtcataata tattcgtagc
    12061 cgaggaaggc atacgaagca taagcaataa cgcgcggcaa cagcgaggtg aaaaagaaca
    12121 ggtttacgaa ccaataggta taggcagtaa taaatgccca acgtccgccc agcgaacttt
    12181 ttacccacgc gtagacacca gcttctgagt ttttatttaa cgaaacaaat tctgcgatga
    12241 tcagacagaa gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca
    12301 gtccaagctc aatattatta ttgataacgt tgttaaagct gtaaactgcg gcaaaagtca
    12361 gcgagagtaa gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc
    12421 gaaaatgtgt agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt
    12481 atggaaataa ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca
    12541 gataaaccga tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctcaac
    12601 ggtttctccg cagcctttta aatattcacg gtcagtttca tcacgataat attccactac
    12661 ctgtaatgtc tctttcggcg catattcaat tttttgctgc ccttgcaggt agtaatgcac
    12721 ttcaaaatag cgtcgatgtc cggtaaatag cgcatcggtc gcggagtcat tttccacacg
    12781 gtaggtcaat gaatcaccga tggagtgggc gacgctaggc tgaatgttgt cgatattttc
    12841 aatcgcttca acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc
    12901 taagttatcg atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct
    12961 gcgccgaact catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc
    13021 aacgtaaagc cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg
    13081 ccccaggagt tggagccgag gccaagcagc tggtggtcga gattcagggt gatgtcatca
    13141 ctgcgctgca actcgttaca gtgctgggca gcgtggatgt tttcctgggt gtagtgccag
    13201 gcactgaagt taattgggcg ctgcggaacc accagcagac cgttgccgtg gcggttagtc
    13261 agtgccgtcc agcggacatg ctgacggttg ccgttgttct gcgggaaggg atagttctcg
    13321 aacatggcat cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg
    13381 tagttttcgc ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaatcccc
    13441 atagtgaaac cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc
    13501 gccacgttaa cctggccatc ggcagcgatg cgccagatgt aggtgcagcg catcccgaaa
    13561 tcaaacaccg gcggggcaat aaccgtgcgg ctgatgatca acacttcacc atcgctctgt
    13621 tctacggcaa agtcgcgcag atgttcctgc atgatctgca aatgattcgg ttgccacagc
    13681 ccttcgtact cctgcttgtg gttgtcgatc atcggcttga agaagttgat ctttggctcg
    13741 cgggtcagca gcgactcgcc atttacctgc caggatgtcg gtttgccact catttttgag
    13801 aaggtgatcg cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc
    13861 agcggacgcg cattatttgg tgcgaaaggc actggctgcg cggtgttttc cttcagcggg
    13921 aactgataag tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc
    13981 accgtaatgt tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat ctgtaagggg
    14041 gcttcgctat tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct
    14101 tcggcgcgca cctctgcgtg cagggtgtag tcatcaagcg tggtaaacca cagtttattt
    14161 tcgactttca gctcgccgcg agtcagatcc agcgcgtgga tttttactgg cgcgataacc
    14221 tgtttgtact ctttcaggcc cggtcccggc gtctgatcgg aatagatcaa accatcaaga
    14281 cagaagttat agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacattg
    14341 ccgttgtcat cctgcgcctg gatcccgtgg tcgcaccatt cccagacata atgtccctga
    14401 atgcaatcgt gcttatagaa gacgttctgg tactccgtca gcccgcccgg tccgttcccc
    14461 atcgcatgag catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca
    14521 ttcatcagcg gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga
    14581 tcttcttcgt aatgcaccag tcgcgtgtca tccagcgcct tcgccgcatg gtacatcgcg
    14641 cggatgttac agccatagcc ggattcattg cccagcgacc agatgatgat cgaaggatgg
    14701 tttttctgcg cgtgaatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga
    14761 tcgtcggtaa tacggctgat atcgccgacg ttagcaaagc cgtgcgattc gacgtcggtt
    14821 tccgccatca caaacaggcc gtagatatca cacagttcgt aaaaacgcgg atcgttcggg
    14881 tagtgagcgg tacgcacgga gttgatgttg tgctgcttca tcaactggag atctttctcg
    14941 acgcgatcca ttccaacggc gcggcctttg cgatgatcgt tgtcgtgacg gttgacgccg
    15001 tgcagcatca cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg
    15061 aagccaacgc gttgtggcac cacttccaga acgttgccgt cggcgtcttt cagcgtcatg
    15121 accaaatggt aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa
    15181 gcaaagctgg cgctggtcag tttttcaatt gccaaatgat caatggcgct actgtgcacc
    15241 acgcgttcgc catcaaacag ggtatattcc agcgttgtga cgacagggga ggcggcgaga
    15301 ttttccagca ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta
    15361 cgaacggtga aatcgttaat atgcgtgagt tgctttccga ccagataaac atcgcggaaa
    15421 atccccgccg accaccacat atcctggtct tccacgtagg tggaatccgc ccactgcatc
    15481 acgcgcacac acaacaggtt gtcgccagtt ttaaccatcg cgctgatgtc aaactctgcg
    15541 gtcaggcgac tgcccttgct gaaccccaca tactgaccgt taacgtagac ttcaaaatag
    15601 gtttcgacgc cgtcaaattt gatcagcgtc tgtttaccct gccagccgtc gctgagggtg
    15661 aaaatacgtt gataggcacc ggttgggtta tcgctgggga caaacggcac atcgatgggg
    15721 aacggaaaac cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg
    15781 gggacggtaa tatgccccca gtcagccatt aactcagagg tgaaggcttc tggcacttgc
    15841 agcggatggt caaaaaagtg gaaattccac tgaccgctta agggcagaaa caggctgctg
    15901 gtttcacggg caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgc
    15961 ggcgcaagtc ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc
    16021 ggtagagaga aatcggaacc attcatggtg atggtagttt gtgtaattgc tggggagttt
    16081 agtaaaattt ttactaaaaa gtagcaggta aatgcattta ctttaactgg atcacgaaaa
    16141 taaacggcag cactgtccgg caaaaacgat tgccggacag tgtgtaaggg gatttaacgg
    16201 gtcgtgccgc gcagttttaa tttgctggga acgaagacta acagcggcag cgcgcgacca
    16261 tcgcgggctt tttcatacac caggttaacg ccctggcttc ccatcatttc ggaatggatg
    16321 cgcacggtgg agagcggcgg aaaggtaaat cgcgcggtgg ggatatcatt aacgctgata
    16381 agcgaaatat cctgtgggat gttcaggcca cgttcatgaa tcgcccgcag tacgccgata
    16441 gcaatggaat cggaagcaac aaacagtgcc ttcggatagt cttcccgcgc caacatttgt
    16501 tttgccagtt catagcccga cgaactggaa aagcctccgc gccagatatc ctcttcgcgt
    16561 accacttgtt tcagtcggcc atattccgca aaggcgacct cacgaatatc cgccttgcca
    16621 ggctcatctt caccgccaat aaaaccaatg cgattaacgc cctggttgat atagaagtcg
    16681 atgatttctt tactgatgcg tgccagatcg atatccaccg catcgtaacc gctgccgggt
    16741 tcgtgaaagt cgataaaaca gatattgtcg gtcaacgcac aggcggcggc gcgcagggcg
    16801 ggcgtgggtt tgccgacaat taaaataccg gtgacgtttt taatgtctgg taatccgctg
    16861 tgttcataac agttggtcag ttcgatcgcc agcttttcgc actgagtttc aatgccgtgg
    16921 cggatcgcca gatagtaagg atcgttgatc tccagctcct gttggtagct gtagatagcc
    16981 agaatatggt gttggttgac tgcacctgtc tggagtttac gggcactact ggtcttgtac
    17041 tccagctttt cggcgatctc gagaatgcga tgtttcgtct cttctttcac attcaatgtt
    17101 ggatcgtcat ttaagaccct ggatactgtc gccagggata cgccagcttc gattgcgatg
    17161 tcttttagtg ttgccatgtt tacctttttt actttccctg atgtgcgccc ccattgtaga
    17221 ggaagtgctt atcgggtcac ctgttttcag taaataaagc gcgtttcttt gacgctgtaa
    17281 cggtaacaca gcggctcacg aatgtgatca cgcctgcatt ctccattttt agcctacagg
    17341 attgagaaaa acttttgcta tagtttagta aaattttacc cgaagggagg aaacaatgga
    17401 aaccgtggct tacgctgatt ttgcacgtct ggaaatgcgc gtcggaaaga ttgtggaagt
    17461 gaaacgccat gaaaacgccg acaagctgta catcgtacag gttgatgtgg gggaaaaaac
    17521 actgcaaacc gtgactagcc tggtgccgta ctacagcgaa gaagagctga tggggaaaac
    17581 ggtggtggtc ttatgcaatc tgcaaaaagc gaaaatgcgt ggtgaaacgt cggaatgcat
    17641 gctgttgtgt gcggaaaccg atgacggcag tgaaagcgtg ttgttaacgc cggagcggat
    17701 gatgcctgcg ggcgttcgta tcgtataaaa caatcggatg gcgacgtcga atcgccatcc
    17761 gatttgatat tacgcttctt cgacacttac tcgcatggcc gccagcgcct tacgcgccgc
    17821 tttgatcacc agttcacact gttcaatggt cagtgtcagt ggcggttcaa tgcggatcgt
    17881 tttggcgtta ttgagcgttc cggccaccag aacgcgctgg cggaacatct cgctggcaaa
    17941 gttatagccg atttcgttat caacaaactc aatcgccatt aacatccctt taccacgcgc
    18001 ttcctgtacc agatcgggat attcccgcgc cagttgacgg aaaccgtcca gcaacatatc
    18061 gcctttttgt tcagcctgag ccggtaagtt ttgttccagc aacacattga tggtcgccag
    18121 cgccgccgca caggccagcg ggttgccgcc aaaggtggtg gtatgcagga acgggttgtc
    18181 gaacaggact gagaacacct cttcagtagc gatggttgcg ccaatcggca tcacgccgcc
    18241 gccgagggct tttgccaggc acaggatgtc cggctgtacg ttctcatgtt cgcaggcgaa
    18301 catcttgccc gtgcgcccca taccggtttg tacttcatcg aggatcatca gtgcgccgaa
    18361 ctcatcgcac agcttacgca cggcggtgag atagcccggc ggcggcagaa ttacgccacc
    18421 ttcaccctga atcggttcga ggatcactgc agccacatca tcaccggttt ttttgcactc
    18481 gctaagggcc gtgcgcatgg cttcgatatt gccaaacggt acatgacgga accctggcag
    18541 cagcggcata aatggtttgc ggaaggtcga tttcgccgtg gccgacagcg cgccaagtga
    18601 tttaccgtgg aacgcgccgc tggtggcaat aaaagtaaac ttgccgcgcg gtgactgata
    18661 agccttcgcc agcttcagcg ctgcttcaac ggactcggtg ccgctattac agaagaagct
    18721 gtatttcagt ttaccgggcg ttagcgccgc aagggttttc gccagcatcg cccgtaacgg
    18781 atcgagcagc tcctggctgt gcagcggttg tttcgcaagt tgattctgta cggcggaaac
    18841 cacaactgga ttacggtgcc ccacgttgaa aattccaaaa cctccaaggc agtcgataaa
    18901 ctcctgtccc tgggtgtcga caagcgtatt taaacctccc gcttgccact ctacggctcc
    18961 gtaatccccg ccggcggtaa cagatttgcg atactctaaa aaccccggat tgacatgctc
    19021 tttgaagtat tcaatcacct cccggttaag tgctttcatc tcctcatgat ctagtgttcg
    19081 cttctcaatg agattcaggg cgtgggcgct gcacgctaaa gccgatgcgc tcgaaggtaa
    19141 cctgttcaaa atatgctccg gaggctcgcg tatcacatga taccgattta agtattgcag
    19201 ggattgcgcc actccggctg cgatcgcgaa aatcgggata aacacaaatg tggttatcat
    19261 ttgcgcaata tttaatgcta tttgttaaca tctgttgtta ttttgcacgg tttttgtcgg
    19321 gtatggattt ggcgattgct gcgcaaatgc acactttcag ggcaacaact gcctgatttt
    19381 tgggcaagat aattaacgtt ttcgtatagt aacagagtga aagtatttta aatcaaggga
    19441 ttagatttat gctttaatcg caaattgcga tctaaatcaa attaatcagt taaagataac
    19501 cgcagcgggg ccgacataaa ctctgacaag aagttaacaa ccatataacc tgcacaggac
    19561 gcgaacatgt cttctcatcc gtatgtcacc cagcaaaata ccccgctggc ggacgatacc
    19621 actctgatgt ccactaccga tctgcaaagc tatatcactc atgctaatga cacttttgtg
    19681 caggtgagcg gctatacctt gcaagagtta caaggtcaac cgcacaacat ggtgcgtcac
    19741 ccggatatgc caaaagcggc gtttgcggat atgtggttca ccctgaaaaa aggggagccc
    19801 tggagcggca tcgtgaaaaa tcgccgcaaa aatggcgacc attattgggt gcgggccaat
    19861 gcggtaccga tggtgcgcga gggaaaaatc agtggctata tgtcgattcg cacccgggcg
    19921 acggatgaag agatcgcggc ggtagagccg ctgtacaaag cgttgaacgc cggacgtacc
    19981 agtaagcgta ttcataaagg cctggtggtg cgtaaaggct ggctgggtaa actgccttca
    20041 ttaccgcttc gctggcgggc gcgtggcgtg atgaccctga tgtttatctt gctggcggtc
    20101 atgctttggt ttgttgctgc cccagtggtg acgtatatcc tctgtgcgtt agtggtattg
    20161 ttggcaagcg cctgttttga atggcagatt gtccgcccga tagaaaatgt cgcccgtcag
    20221 gcactgaagg tggcgaccgg agagcgtaat agtgttgagc atctgaatcg cagcgatgag
    20281 ctggggctga cattacgcgc ggtagggcag cttggcctga tgtgccgttg gctaattaac
    20341 gatgtctcaa gccaggtgtc cagtgtcaga aatggcagtg agacgctggc gaaaggcacc
    20401 gatgaactga acgaacatac ccagcagaca gttgataacg ttcagcaaac ggtggcgacc
    20461 atgaaccaaa tggcggcgtc ggtgaaacag aactctgcca cggcgtcggc tgccgataaa
    20521 ctttctatca ctgccagtaa tgcggcagtg cagggcgggg aggcgatgac cacggtgatc
    20581 aagacaatgg acgatatcgc cgacagtacc cagcgcattg gcaccattac ttcgctgatt
    20641 aacgatattg cgtttcagac caatattctg gccctgaatg cggcggtgga agcggcacga
    20701 gccggcgaac aaggcaaagg ttttgcagtg gtggcagggg aagtgcgtca tttagccagc
    20761 cgcagcgcca atgctgccaa cgatattcgc aagctgattg atgccagtgc tgataaggtg
    20821 caatccggtt cgcagcaggt acatgcagcc ggacggacga tggaagatat tgtggcacag
    20881 gtgaaaaacg tcacccaatt gatcgcccag attagccatt caacgctgga acaggccgat
    20941 gggctttcca gcctgacccg tgcagtggat gagcttaacc tcatcaccca gaaaaatgcc
    21001 gagctggtgg aagagagtgc gcaggtgtcg gcgatggtaa aacaccgcgc cagccgactg
    21061 gaagacgcgg tgacggtgct gcattaatcg atatgccgga tccggtgttt gatacacgat
    21121 gcctgatgcg atgctggcgc atcttatcag gcctacgggg tgtgtagggt acgccgcatc
    21181 cggcaatcgg tgattaatcc agttgcgtaa tatcaaaagc ggcgcggtcg atcaccgcaa
    21241 tgatcttttt tatctgcgca tcagtgatat cgctctggtt gacgcgtaaa tccagcaccg
    21301 ctttaaaatt atccagcgcc cgcttcattt gcgggttctg gcggagtgca gcaccaacgc
    21361 aacgcgcttt gatgcgttct tcgatcatct ccacctgttc gcggttttct tccagccact
    21421 gcgcgccctg ttcggtcagt gcaatctgct tcttaccacc ttcctcttcg cggatggtaa
    21481 tcagcgactg ctcctgcaga aaatccagcg ttgggtagat gacgcccggg cttggggtgt
    21541 aattcccctg ggttagattc tcaatcgctt taatcaattc gtaaccgtgg ctgtcatcgc
    21601 gcgagagaat atccagaatc accagacgca attcaccgtg accaaagaaa cgctgccgac
    21661 gaccgccgcc gcgaccgtgg cgaccaccgc agcattgacc gtgttcatgc tggtgaccgt
    21721 gtccgcagtg ctcatgttct gacttctcac ctttgcagca gccttcatgg cgtggctggc
    21781 cttcatgttt acaacaccct tcgtgatgat ggctcatatc agcctccttt tgcttaatta
    21841 gatatatcta atttatatct gatatgagcg cttttgcaag tataaaaatg atttttaaac
    21901 gtgaatacac ctcacatcca ggccggataa ttcgttcacg ccgcatccgg cagccttgct
    21961 acgacgctga atgtcttctc atacttcaga tatatcagtt ctacaaatcg cttgcattta
    22021 tcatgattaa caatcattat catttgcgaa atttatttag atatatctga ttactatcac
    22081 gaaggcgata acaatgaata actccccccg ctacccgcag cgcgttcgca atgatctgcg
    22141 cttccgtgaa ttgactgtgc tacgcgctga gcgtattagc gccggttttc agcgcattgt
    22201 ccttggcggc gaggcgctgg acggttttac atcgcgcggc tttgacgatc acagcaaact
    22261 cttctttcct caatctgatg ctcactttgt gccgccaacg gtaacggaag agggcatcgt
    22321 ctggccggaa ggaccacgcc caccgtcgcg tgactatacg ccgctgtatg acgaactacg
    22381 ccatgaactg gcgattgatt tctttattca cgacggtggc gtcgccagca gctgggcaat
    22441 gcaggcgcaa ccgggcgata aactcacggt ggcaggtccg cgcggttcgc tggtggtgcc
    22501 agaggattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg cactgcgccg
    22561 ccgcctggaa acgttgagca aacttgctgt taaaccgcaa gttagcgcac tggttagcgt
    22621 gcgggatcac gcctgccagg attatctcgc gcaccttgat ggttttaata tcgaatggct
    22681 ggcgcatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc ctgccgatga
    22741 ttacttcatc tggataaccg gcgaaggcaa agtcgttaag aatttaagcc gccactttga
    22801 agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg caaagtaact
    22861 gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat ctggtggccc
    22921 ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta
    22981 aggggccatg gtagcggatt ataaagtaac tccgtgtcgc aatccagcca ttaccgcgcg
    23041 cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg tgatgttaga
    23101 aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg ttttgcggta
    23161 tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg gcaaatcgct
    23221 tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg caggaggcac
    23281 agcatctgct ggattatcgt tgtggcgtca ccaaactggt agaccgtcca acggtgcaag
    23341 ccaatgaagt ttcaaagcag gagctacacg caggcgggcg taagctgatt gaaaaaattg
    23401 aagattatca gccgcaggcg ttggcgattc tgggcaaaca agcatatgaa cagggattca
    23461 gccagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg cagatttggg
    23521 tgctgccaaa tcccagtggt ttaagtcgcg tttcactgga gaaactggtt gaagcgtatc
    23581 gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa aacgccaccg aaaaggtggc
    23641 gtttgtgcag agggcctagt gccgggtgcg gcgtaacgcc tgatccggcc taccgattaa
    23701 tcgtccagga agctacgcag cacttcagaa cggctcgggt gacgcagttt gcgcagcgcc
    23761 ttcgcttcga tctgacggat acgttcgcgg gtaacgtcga actgtttacc cacttcttcc
    23821 agcgtgtggt cggtgttcat atcgataccg aaacgcatac gcagaacttt tgcttcacgc
    23881 gcggtcaggc cagccagcac gtcgtgcgtt gccgcacgca ggctttcggt ggtcgcagaa
    23941 tccagcggca gctcgagggt ggtatcctcg atgaaatccc ccagatgcga atcttcatca
    24001 tcaccgatcg gcgtttccat ggagattggc tctttggcga tcttcagcac tttgcggatc
    24061 ttgtcttccg gcatcagcat acgttcagcc agttcttccg gcgtcggttc acggcccatc
    24121 tcttgcagca tctggcgaga aatacggttg agtttgttga tagtctcaat catatgcacc
    24181 ggaatacgga tggtgcgcgc ctgatccgcg atagaacggg tgatcgcctg acggatccac
    24241 caggttgcgt aggtggagaa cttgtaacca cggcggtatt cgaatttatc aaccgctttc
    24301 atcaggccga tgttaccttc ctgaatcagg tcaaggaact gcaagccacg gttggtgtat
    24361 ttcttggcga tagaaataac cagacgtaag ttcgcttcaa ccatctcttt cttcgcacgg
    24421 cgggcttttg cttcaccgat ggacatacga cggttgatat ctttaacctg ctcgatggtc
    24481 aggccggttt cttcttcaat ctgctgcagt ttctgcaggg cgcgatgcac ttcttcagag
    24541 acatcgtgca gtttttccga ccacggcttg ttcatcgcaa ttgccgcgtt gaaccaggta
    24601 tcgctggttt cgttgccggt aaacagggta atgaagtttt tcttcggcat tttgcactgc
    24661 tcaacacaga gcttcatgat cagacgttct tgcgtacgaa cgcggtccat catgacgcgc
    24721 atgctgttga ccaggtagtc aaactgcttc ggcaccaggc ggaactgttt gaatacttca
    24781 gacagtttca ggatctcttc ctgagcggca gcgtgactgc gacctttcgc tttgatggtg
    24841 tcacgcgtta caacgtactg agcgcgcagt tccgcaaatt tttcacgagc cagttccggg
    24901 tcgatgctgt tgtcatcatc ggcgctgtcg tcatcgccat cttcttcgtc ttcatcttcg
    24961 tcatcgtcca gatcttcctg ggaaagctca gaaccgacgt gagtggcggt aggtgccaga
    25021 tcttcttctg cgttcgggtc aacaaagccg gtgatcagat cggacagacg cgcttcttct
    25081 gcttcaacac gatcgtactg ttccagcaga taggtgatcg cttccggata ttcagcaacg
    25141 gagcattgaa cctggttgat cccgtcttca atacgcttag cgatgtcaat ttcgccttcg
    25201 cgggtcaaca gttcaacggt gcccatttca cgcatgtaca tgcgtaccgg gtcagtcgtg
    25261 cgcccgattt cagattccac gctggaaagc acctgcgcgg cggcttcggc agcatcttcg
    25321 tccgcggtgt tttcagccag catcagatca tcggcatccg gtgcttcttc catcacctga
    25381 atgcccatgt cgttgatcat ttggatgatg tcttcgatct gatctgaatc gacgatatct
    25441 tccggcagat ggtcattgac ctcggcatag gtcagatagc cttgctcctt accacgggtg
    25501 acaagaagtt tcagctgtga ctgcgggttt tgctccataa gacggtatcc acacttattt
    25561 catgaggttg gtgttgggcg attgacccga tgtccttcag ccgttaacgc tgaaatccaa
    25621 cggttgttta ccgccaacga taattacgag ggcgtactta tatatttgcc gctgcctctc
    25681 agtgcggctg tcgggggctt cccgatcgct cttcggcact taagccgtta aatcactttt
    25741 tcgccagctc ctggtttaat gtccagagct ccaggcgttc ttcgttgctt aaaccatgcg
    25801 tgcgctcacg agcgattaac tcttcctggc gcagttcaag cagcgaatca aacatatggt
    25861 tgagtgagtc ggtgaaggtt tgctcagcaa tattcttatc tgctatatcg tcccacatcg
    25921 acagtttttc aagggtggca gcattatttg taccacgata gtgctctaaa agttgcccgg
    25981 tggtcagacc tggctgagag agacaagtgt tgaccagttc tctgaataag ccaagtccag
    26041 ggagcttatt ttcatccaga ttctcaagcg gcgggaccaa cgtcgctaat tctggatttt
    26101 gcaccagcaa ccctataagt atacgcatgg tcgtgcgttt tagctgcgga acagggcgag
    26161 aaacgccgct ctctgccgct tttggcatta atcgttcaag ctggctgtca tcaagtatgc
    26221 ctaatttgtt gcctaattcc tgacgaagat atattcgcag cgtttcgccc ggcacttgcg
    26281 atatcaatgg cagtgccagc gtactcaaac gtgcgcgccc gtcaggggta ctcagatcaa
    26341 cttgcggcat caggctgtta aacagaaatg cggagagcgg catcgcctgc tccatccgcg
    26401 cttcaaacgc ttgtttacct tcttttcgta ccagcgtgtc agggtcttcg ccatcaggca
    26461 aaaacataaa gcgtagctga cgaccgtctg tcatgtaagg cagcgccgtt tccagcgctc
    26521 gccaggcggc atcgcggcct gcacggtcgc cgtcataaca gcaaatgaca ttgttggtcg
    26581 cgcggaacaa cagttgtatg tgatcggcgg tggttgacgt acctaacgac gcaacggcgt
    26641 aattaatgcc gtattgcgcc agcgccacca cgtccatata gccttcgacc acaagcagac
    26701 gattgggttc agcgttatcc tgctgcgctt cataaagacc gtaaagctgg cggcctttat
    26761 ggaaaatgtc cgtttccggc gagttcaggt atttgggggt atcgttgccc agcacgcgtc
    26821 caccaaaacc aatcacccga ccgcgtttat cacgaatggg gaacatcacc cgctcgcgga
    26881 aacggtcgta actgcgcccc tgatcgttag tgaccaacat gcccgcatca atcaatgact
    26941 ggcgattttc tggattgccg ccaaaccgct tcaggacgtt gtcccagccg gggggcgcaa
    27001 aaccaatcgc aaagcgggcg ataacctcgt ggcttaatcc gcgtttttcc agatactggc
    27061 gcgcagacgt ggcaacaggt tgctgtaaag attgttggta aaacgtattc agaccgtcca
    27121 tcaactgata aagcgtttgc ctctgatggc gctcgatctg gctggggccg ctgcctgctt
    27181 caaatggcac ttcaagattg tgcattgctg ccagctcttc gaccgtttcg acgaactcga
    27241 gcttgtcgta gttcatcagg aagtcgatcg cgttgccgtg cgcgccacat ccaaagcagt
    27301 ggtaaaactg tttctcaccg ttaacggtga atgacggggt tttctcgttg tggaatggac
    27361 aacacgcgtg gaaattcttg ccctgctttt tcagcttcac acgggcatcg atcagatcga
    27421 cgatgtcagt gcgtgccagc agatcattaa tgaatacgcg tgggattcgt ccagccatag
    27481 gccccgattt ttagcaattc ataaacgaaa ataagccgcg cattcctttc ggaagcacgg
    27541 ccttacaact acaactcggt ctgatcggag agcaacgctc tcggggaatt agtacagacg
    27601 agtgcggcgt gcgttttcgc gagccagttt cttcgcgtga cgtttcactg cagaagcttt
    27661 agcgcgctta cgttcggtag tcggtttttc atagaactca cgacgacgaa cttccgccag
    27721 aacacctgct ttttcgcagg aacgcttgaa gcgacgcaga gctacgtcga acggctcgtt
    27781 ttcacgtact ttaattaccg gcatgtgcct ctcacctttg attaattcgg tttgccgctg
    27841 gcatcaacgc cagcttattt caaaatggtg cggaatttta ctgcaattgc tgctgctttg
    27901 taaagcaccg cggccttttt tgaaagggac ttttataagg gtgaggagta tacacgaggc
    27961 tttctccagg ggcgaacaaa gttttacatc aacccgcatt ggtcctacac tgcgcggtaa
    28021 taaagcgagg taaaacaagt catgcgtgta ctgggtattg aaacttcctg cgatgaaacc
    28081 ggcatcgcca tttacgacga tgaaaaaggt ttgttagcca accaattgta tagtcaggtg
    28141 aaattgcacg ctgactacgg cggcgtcgtg cctgaactgg cctcccgcga tcacgtgcgt
    28201 aaaaccgtac cgttgatcca ggcggcgcta aaggagtcag gtttaacggc aaaagacatt
    28261 gatgctgtgg cctataccgc aggccctgga ttagtcggcg cgctgctggt tggcgcgacc
    28321 gtggggcgtt ctctggcgtt tgcctgggac gttccggcga tcccggtaca ccatatggaa
    28381 gggcatctgt tagcgccgat gctggaagat aacccaccgg aatttccgtt tgtcgcgctg
    28441 ctggtttccg gcggtcatac gcagttaatc agcgtgactg gcattggtca gtacgagctg
    28501 ctcggcgagt ctatcgatga tgcggctggt gaagcgtttg ataaaaccgc gaagctgctg
    28561 gggctggatt atcctggcgg gccattactg tcgaaaatgg cggctcaggg tactgccggg
    28621 cgctttgtct tcccgcgccc gatgaccgac cgtccggggc tggatttcag cttttctggt
    28681 ctgaaaacct tcgcggcaaa taccattcgt gataacggca ccgacgacca gacgcgtgct
    28741 gatatcgccc gcgcctttga agatgcggtg gtcgatacgt tgatgattaa gtgtaagcga
    28801 gcgctggatc agacgggctt taagcgactg gtaatggcag gtggcgtgag tgctaaccgc
    28861 acgttacggg cgaagctggc tgaaatgatg aaaaaacgcc gtggcgaagt gttctacgcg
    28921 cgtccggaat tttgtactga taacggcgcg atgatcgcct atgccggaat ggtgcggttt
    28981 aaagcaggcg cgacggcgga tcttggcgtt agcgtgcgtc cgcgctggcc gctggcagag
    29041 ttaccggccg cgtaaaacta ttaggtgccg gagagtcact tccggcaccg tcctcactta
    29101 aagcaacacc atgggcatcc acagtaagcc ggtaatcacc agcaacacga ggaatatcag
    29161 cccaaaaatt gcccccagtc gccagtaatc caccgttggc agataaccac taccgtagta
    29221 aatcggactg ggtccggtgg cgtatggcgt aagaatgctc cccagaccaa ttgccgcgcc
    29281 aaccatcagg cagaacacgg gaagcgggat ttccggcatc gccagcgcgg cggcaatcat
    29341 catcggcgcg agagcggagg tatacgccgt ggcgctggca aagaagtagc gcagcagata
    29401 aaagaccaca atcaacgcca ccatcaccat cgttggcgaa taaccgttta agctgcctgc
    29461 taacagtttg ccaaaccagc taataaaacc ggtgttgttg agtccggtag cgagggtgat
    29521 aagcgaggcc agccagaaga aaacgttcca cgccgcttta ttactgacaa tgtcgtccca
    29581 actgataatg cgcagaagca gcatcagcgc caccacgctg taaccgacca tcgcggcatc
    29641 gatataatca ccgccgaaaa tccacagcac cagcgcgcct accatcagcc ccagcatccg
    29701 tttttcacgc gaacagagcg ggcccattgc ctgcagttcc gtctctgccc agcgcggcac
    29761 ctgatcacca gacttcagta ccggcgggta cagcacgtaa gccagccagg gaaccagcag
    29821 aaccagtaaa atactgagcg gcaacatccc gaggaaccag tcgccccaac tcagtgtggc
    29881 gtgagatgcg cttttcatca ggccaattaa cagcaagtta ggtgccatcg ccgtcaggaa
    29941 aatggcgctg gtcacgcagt cggcaacaat ccccatccac atgatgtacg agccaatgct
    30001 gcgcgaactg ctgtcgtttg gttgtgattg atagagcggt ggcaggttac ggatgatggg
    30061 atagataatc cccgcaccgc gcgccgagtt ggacggtgtt acaggtgcca ggatcagctc
    30121 ggagaacatc accgcatagc cgagaaacag cgtacgatgc cccatctttt tcaccagaat
    30181 cagcgcaatg cggcgtccaa gcccggtttt ttcatagcct gtgccaaaca taaaggcggc
    30241 gaaaatcagc cagataaccg aattagaaaa accagaaacc gcccacgaga gggatttggc
    30301 ggtgaactta aaacctggtt gggcgagctg ctccgggcta aacagcagcc acggcgagag
    30361 aatggcgata atggagatac ccaccatcgc caccacggcc cccggcacgg gttcgaggat
    30421 cagtccaacg atcacgccag taaaaacggc aaagtagagc caggtatgac tctccagccc
    30481 tgcgggaacc ggaattagag caataatggc gatgaccgcc agcggcgcaa ggtatcgcca
    30541 ccattcagtg gaaggtttca tcgtgttatg cctgcgaaaa aggagagggg gtgagcctct
    30601 ccgggagggt tatttgatgt agtggacgtg ctcgcaaatc tcttccacga tgggatcgcg
    30661 gcgttcggcg aacagctttt tgttttcggc tatcaggttg ttgccgtggg tatcaataga
    30721 gacaatcagc ggaccgaact ctttgacccg gcagacccac agcgactccg gcatcccgag
    30781 ttctgtccag tgcacttctt caatctcttc cacctgggtt gccgccagta ctgcgcagcc
    30841 tgccgggaaa atcacatgta gcgccttgaa tttctgacag ccttcttcgg tcagcggccc
    30901 cattccgcct ttgccgacca ccagtttcac gccggtctgt tcgataaatt cacgttcaaa
    30961 actttccata cgcatactgg ttgtcgggcc gacggatacc atctcccatt tgtcgccgtt
    31021 tttgcgcacg atggggccag cgtggaaaat cgctttgccg ttgagatcgt aagggatcgg
    31081 acgtttcagt tcgatcaagc ggcggtgaca aacgtcgcgg caggttacca gcgtacctgt
    31141 caggtagatc acatcgccga cgcgaatatc ttgcagatct tcagctttga tcggggttgt
    31201 caggatcttt ttcataacgc gctccgggtg tgagacagat tttcaaaggt gagatcggca
    31261 tgaaccagca gtgtgccgcg acgatgcgcc cagcagcctg tagaaacagc aacgccgatg
    31321 gttgacggat ggcgggcggc agattcgata tgtacgccca tcactgaact gttgccagtc
    31381 agcccttgtg gaccaatccc caaacggttg agtccttctt ccaggcgtag ctccagctct
    31441 gccgctttcg gattggggtg gcggctgcca atcgggcgca aaatggcttt acgcgagagc
    31501 actgcggcgg tttccaccga ggtggcgatg cccacgccta ccagtaccgg tggacaggcg
    31561 tttacggcga gggtggaaat attttcgaag acgaatttca ctacgccttc gtagccttct
    31621 gacggcatta acactttcga gcggccaggt agcgtgcagc cgccgcctgc catgtaaact
    31681 tcgatttccg catcgtcacc gtcggggacg atatcccagg tgacccacgg tacgccgctg
    31741 ccggtatttt tgtcggtgtt cacttcgtca aaaatttcca ccgcgttgtg gcgcagtggc
    31801 gctttgacgg ttgcttcttc cacagcttgc ttaagaatgc tttgcagctc gccaagcagc
    31861 gggaaacggg aaccgacttt aacgaagaac ataatctccc cggtgtcctg acaggcagga
    31921 cgattcaggt cgattgcttt ttgcatgttg tcgaacatcg tgtggtagat gattttcccc
    31981 atcgacgacg tttcggcatc ctttagctgt tttagtttat ccaccacgtc atcgggcatt
    32041 cgggtagaaa tcatggcggt aaagttagcg acaatctccg tcaacttatt cactgcctgt
    32101 tgcttattac tttcgctcat catggcaact ccagtttatg tatttgtctg gaaaaattca
    32161 ccgccacaaa ctaccactaa aaatgtggtt ttatattttt tgccaccgtg aatatgtaat
    32221 tcacagttcg tgaatcaacg cgaaaataag aatgaatatt gcgccagagc agggaatggc
    32281 aatgttttgc gaaatacact gcgcttatca caactgcgga taattaacga tgctgaatag
    32341 ctggccttta gccaaagact tgcaggtact ggtagaaatc gtccacagcg ggagttttag
    32401 tgctgcagcg gcgacgcttg ggcaaacgcc tgcatttgtc accaagcgga tccaaatcct
    32461 tgaaaatacg ctggcaacaa cgctgctgaa ccgctccgct cgcggcgtgg cgctgactga
    32521 aagtggccaa cgttgttacg agcatgcgct ggaaatcctc acccaatatc agcgactggt
    32581 cgatgacgtt acgcaaatca aaacgcgccc ggaggggatg attcgtattg gctgtagttt
    32641 tggttttggg cgcagccata ttgcgccagc cattaccgaa ctgatgcgca attatcctga
    32701 gttacaggtg cattttgaat tgttcgatcg gcaaattgat ttagtgcagg ataatattga
    32761 tctggatatt cgtattaatg acgaaattcc tgattattat attgcgcatc ttttgacaaa
    32821 aaataaacga atattatgtg cagcacctga gtatctgcaa aaatatccgc agccacaatc
    32881 cttacaggaa ttaagtcgtc atgattgtct ggtgaccaaa gagcgtgata tgacccacgg
    32941 aatatgggag ttggggaacg gtcaggagaa aaaatcggtg aaggtaagcg ggcatctttc
    33001 ctccaatagc ggcgagattg tgctgcaatg ggcgctggaa ggaaaaggaa ttatgctgcg
    33061 ttctgagtgg gatgttttac cgtttctgga gagtggcaaa ctggtgcgag tattgccgga
    33121 gtacgcacaa agcgccaata tctgggctgt ttaccgggag ccgctctatc gcagcatgaa
    33181 attacgtgtc tgcgtggaat ttctggcggc atggtgccag caacggctgg gcaagcccga
    33241 cgaaggctat cagattatgt agatccacca gaaatcactc gggatccttt tcgcgctttc
    33301 ttttgaattt cgtccagatt tttgtctcct gacgacgcca cagacgctgg atgttgtcat
    33361 gatgacgcag caggatcagg caagagagca tcgaaaccgg gaaggtgaat tgtggcttaa
    33421 accaccagac ataaaacgga gcaatcagtg cactgacaat cgctcccagc gacgaatatc
    33481 cgctcaatag cacggtcagt aaccaggttc ccgccattac tccggtgaga tcccagccaa
    33541 ttggtgcgat ggcaccaaaa gcggtagcaa cgccttttcc tcctttaaaa ccgaagaaaa
    33601 cgggccagat atgtccaaga caggcggcga tggcaattaa gcctagccag aaggggctga
    33661 cacctaattc atacgcgccc cagacaggca acattccttt cagaacgtcg aaaatcagta
    33721 ctgctacggc tgctcccttg ccaccgatac gtaacacatt ggttgcgcct ggattgccgg
    33781 agccgctggt tcgcggatcg ggcagcccac acaagcggca aaccagaatg gcactggaga
    33841 tggagccgca gaggtacgcg atgaggatca ttccaggcgc gattgcactc ataagctgtt
    33901 ccgttttgaa aattcgtgtt caacgatgaa tctgtggata atacgcacat ttcgccggaa
    33961 gtggtatccg gttagccaaa aagcaggcag gacgtgatgg atattgtatt tatagagcaa
    34021 ctttcggtaa tcaccactat tggtgtttac gactgggaac agaccatcga acagaagtta
    34081 gtgttcgata tcgaaatggc gtgggataac cgtaaagcgg cgaaaagtga tgatgtggcg
    34141 gattgcctca gttacgctga cattgcagaa acggtagtca gccacgtcga gggggcgcgt
    34201 tttgcgctgg tggaacgtgt ggcggaagag gtggcggagc tgctgttagc acgcttcaac
    34261 tcgccgtggg tgcgtatcaa actcagcaag ccaggcgcag tggcgcgggc ggcgaatgtt
    34321 ggcgtcatca ttgagcgtgg caataatctg aaagaaaata attaatttta cagctgttaa
    34381 accaaacggt tataacctgg tcatactgca gtagttcgga caagcgttac atttttataa
    34441 tttaggggtt tattgatgag cgatatgcac tcgctgctga tagcggcaat attgggtgtg
    34501 gtcgaaggat tgacagaatt tctgccggta tccagcacgg gccatatgat tattgtcggt
    34561 cacttgttgg gatttgaggg cgacacggcg aaaacctttg aagtcgtgat ccagttagga
    34621 tcaattctgg cggtagtagt gatgttctgg cggcgtctgt ttggcctgat tggcatccac
    34681 tttggtcgcc cgttgcagca cgaaggagaa agcaaaggcc gtttaacgct gatccacatt
    34741 ttgctgggga tgattccggc ggtggtactg gggctgttgt tccacgacac gattaaatca
    34801 ttgtttaacc cgataaatgt gatgtatgca ctggtcgttg gcggtctgtt gctgattgcc
    34861 gctgaatgcc tgaagccgaa agagccgcgt gcgccgggtc ttgatgatat gacctatcgt
    34921 caggcgttta tgattggctg tttccagtgt ctggcgctgt ggccgggttt ctcccgttcc
    34981 ggggcgacca tttcaggtgg gatgctgatg ggggtgagcc gttacgctgc ttccgagttt
    35041 tcgttcctgc tggcggtgcc gatgatgatg ggcgcaacgg cgctcgatct ctacaaaagc
    35101 tggggcttcc tgacaaccgg cgatatcccg atgtttgccg ttgggtttat caccgctttt
    35161 gtggtggcgc tgatagcgat taaaaccttc ctgcaattga ttaagcgcat ttcgtttatc
    35221 ccgttcgcca tttatcgctt tattgtggcg gctgcggtgt atgtcgtgtt cttttgattg
    35281 aggaatagtg tcggatatga cgcttgtgcg tcttatccga cctggccggt gtttgcaata
    35341 aattgaaatt caagtgctta atcaggcttt ggacaacgtt gttccttcca gctggctacc
    35401 gccgcaatcc gccgtcgggt cagctcctcg cgaatctcca cacctttaaa tcccgcttca
    35461 acgacggctt ttgttggcac tgactgtgcc acttcccagg cttcgcgcaa ccagcggcct
    35521 tgcgggtagt ccgctgattc aaaaccggtt ctgccgcgca cgtcagcctc gctggtcagc
    35581 gccagttgct cgacacgctg cggtttacgc caggcgtcga tggaatcaaa caatttgacg
    35641 atggtttttg ggttcagcat tgggaaggtg tggatgagat cgtgaaactc agccaccagt
    35701 ctggctaaat cgcgaatttc atttggcacg cgcagacgct gacagagttg ttccactaac
    35761 ttaacacccg ccgggccatg accatgatga cgcggccaaa gttctggtgg cgtcagtcct
    35821 ttaccgagat cgtggcataa agttgcgaaa cggacatcga cctgcggact tagcatcgcc
    35881 gccattgaga gcgtcattaa ggtgtgaata ccggtgtcga tttccggatg ccacctggca
    35941 ggggccggaa cgccaaacag ggcgtcaatt tccgggaata aaacgcgtag tgcaccacaa
    36001 tcgcgcagta cctggaagaa cacctgggga ttgcgggtgg tgagggcgct ttccgtctct
    36061 ttccataccc gttcaggcgt caggtgttcc agttcacccg catgggtcat ctcgcgcatc
    36121 agtgccagag tttcatcggc aatacgaaaa ccgaggtggg cataacgcgc agcaaaacgc
    36181 gccacgcgca atacgcgtaa cggatcttcg ccaaaagcgg gggaaacatg gcgcaacaga
    36241 cgattttgca gatcgcccag accgttgtac gggtcgataa tctcgccgtt atcgtcctgg
    36301 gccagcgcat taatggtcag atcgcgacgc ttaagatcat cttccagcgt gacatccggt
    36361 gcggcatagc aggtaaaacc ggtgtaaccg gaaccggatt tccgttcagt acgtgccagc
    36421 gcatactctt catgcgtttg cggatgcaga aaaacaggaa aatcgcggcc tacctgctgg
    36481 tagcccgcgt cgagcatctc ctgtggcgta ctgccgacca ccacccaatc tctgtctttg
    36541 accggtagcc ctaacaatgc atcccgaaca gcaccaccga ccagataaat cttcacgcca
    36601 tcatcccttc gtcaaataca caattcctaa taataagaca gtgcaacgaa gaaggcgatt
    36661 tagttcatcc agcgatcttt gcgtttgcgg cttgggatca ggtgcggcag taccagaccg
    36721 agcagcaagc caagccccag cacgccgccg ccatacataa accattgcat gatgatggtg
    36781 cgctgtttgt catccagctg tacgctggcg gcatcgacct ttttctgcgc gacaatcagc
    36841 tcgtttttca gtttctgatt ttcttctttt aacccgttga tcacgctgtc gctctgcgcc
    36901 actttttgct gcatttctgc ggtgcgctga ttccaggtgt tatcgatatt ggtgagttta
    36961 tcggtcaggg ttttgacctg attttccaga tctggcacac gggagcgcag gcttggctca
    37021 gtgctaagtt gtttcaacgg gatccaggcg gtacggccag agctgtcttt cacctgggca
    37081 taattggtgt tggcgtcagt ttgtaataag gtcacttcct cgccggcatt aaccgtgccc
    37141 acgaggcgat aatgatctcc cggaccgcta cggacccagg tattcagttc gtcggaaaca
    37201 tagcgcgttt cttcagcgtg tgagacggca gtggcgctaa gtgcgagtaa agttaatccg
    37261 atcaggcgta attttggcat caggctgtcg ttattgtcat ggaaagtgga acgatagtag
    37321 tggcatcagt gtcgctacgc aaagcattcg acatcaatcg gaatcctctg tgtcaggtct
    37381 tgccactgct tcaaactttt acgcccgtca aattgcccgt tgcatggcaa tttggcgcaa
    37441 aatactatct actgacaaaa aagatcgcat taagttcgcc gttcatcgtc acttatgtga
    37501 cataaccgat aagtaaaggc catggctcag gaaatcgaat taaagtttat tgttaatcac
    37561 agtgccgttg aggcgttgcg tgaccatctc aatacgctgg gcggcgagca ccatgacccc
    37621 gtgcagttgt tgaatattta ctacgaaacg ccggataact ggctgcgtgg gcacgacatg
    37681 ggcttgcgta ttcgtggcga aaacggtcgc tatgagatga ccatgaaagt tgcaggaaga
    37741 gtgacaggcg gcttacacca gcgcccggaa tataacgtgg cgttgagcga accgacgctc
    37801 gacctggcgc agttaccgac ggaagtctgg ccgaacggcg aattgcctac cgatctcgct
    37861 tcccgcgtgc agccgctgtt cagcaccgat ttttatcgcg aaaagtggct ggtagaggtc
    37921 gatggtagcc aaattgaaat cgctctcgac cagggggaag tgaaagcggg ggaatttgct
    37981 gagcctatct gcgagctgga actggaactg cttagcggcg acacgcgcgc ggtgctgaaa
    38041 ctggcgaacc aactggtatc gcaaaccgga ttacgccagg gcagcctgag caaagcggcg
    38101 cgtggctatc atctggcgca gggcaatccg gcgcgtgaaa tcaaaccgac caccattttg
    38161 catgttgcgg caaaagccga tgtggaacag gggctggaag cggcgctcga gctggcgtta
    38221 gcgcaatggc agtatcatga agaactgtgg gtacgcggca acgatgcggc gaaagaacag
    38281 gtgctggcag ccattagcct ggtccgtcat acgctgatgc tgttcggtgg tattgtgccg
    38341 cgtaaagcga gcactcactt acgtgatctg ctgactcaat gcgaggcgac cattgcttct
    38401 gcggtgtctg ccgtgacggc ggtctactct accgaaacgg caatggcgaa gctggcgttg
    38461 accgaatggc tggtaagcaa agcctggcag ccgtttttag atgccaaagc gcagagcaaa
    38521 atcagcgatt ccttcaaacg ctttgccgat atccatcttt cccgccatgc cgctgaactg
    38581 aagagcgttt tctgccagcc gttgggcgat cgctaccgtg accagttgcc acgcctgacg
    38641 cgtgatattg actcaatcct gttgctggcg ggttactacg atcctgtcgt cgcgcaagcc
    38701 tggctggaga actggcaggg gctgcgtcac gctattgcta ccgggcaacg catcgaaatt
    38761 gaacatttcc gtaatgaggc aaacaatcag gaaccgttct ggttgcacag cggaaaacgt
    38821 taatcaggca aggataaaat cgcttatgaa gccgctctct tcaccgttac agcagtactg
    38881 gcagaccgtt gttgagcggc tgccagagcc tttagccgag gaatcactta gcgcacaggc
    38941 gaagtcagta cttactttta gtgattttgt gcaggacagc gtgattgcgc atccagagtg
    39001 gctgacggaa ctggaaagcc aaccgccgca ggccgacgaa tggcagcatt acgcggcatg
    39061 gttgcaggag gcgctctgta atgtgagtga cgaagccggg ttaatgcgcg agctgcggct
    39121 attccggcgg cgcattatgg tgcgcatcgc ctgggcgcaa acgctggcac tggttactga
    39181 agagagcata ttgcagcagc tcagctatct ggcggagacg ctgattgttg cggcgcgtga
    39241 ctggctgtat gacgcctgct gccgcgagtg gggaacgccg tgcaatgcgc agggcgaagc
    39301 gcaaccgctg ctgattttag gcatgggtaa gctgggcggt ggggagctga atttctcctc
    39361 tgatatcgat ctgatttttg cctggccgga acatggttgt acgcagggtg gacgccggga
    39421 actggataac gcgcagtttt ttacccgcat ggggcagcgg ctgattaaag tgctggatca
    39481 accaacgcag gatggcttcg tctatcgcgt ggatatgcgg ctgcgtccgt ttggcgaaag
    39541 tggcccgctg gtgctgagct ttgccgcgtt ggaagattat taccaggagc aggggcgcga
    39601 ctgggagcgt tacgcgatgg tcaaggcgcg gattatgggc gatagcgaag gcgtctatgc
    39661 taacgagttg cgtgcgatgc tgcgcccgtt tgttttccgt cgttacatcg atttcagcgt
    39721 gattcagtcg ctgcgcaaca tgaaagggat gattgcccgt gaagtgcgtc gacgtggttt
    39781 gaccgacaat atcaaactcg gcgcaggcgg cattcgcgaa attgaattta tcgttcaggt
    39841 gttccagctc attcgcggcg gacgcgaacc gtcgctgcaa tcgcgctctt tactgccaac
    39901 gctcagcgcc attgccgagc tgcatctgct ttctgaaaac gatgctgaac aattgcgagt
    39961 ggcgtatctg ttcctgcggc gtctggaaaa cctgctgcaa agcattaacg acgaacaaac
    40021 ccagacgctt ccttctgatg agcttaatcg tgcgcggctg gcgtgggcga tggactttgc
    40081 tgactggccg caactgaccg gggcgctgac cgcacatatg accaatgtgc gccgggtgtt
    40141 taatgaattg attggcgacg atgaaagtga aactcaggaa gagtcgctgt cggaacagtg
    40201 gcgtgagctg tggcaggatg cgttgcagga agatgacact acgccagtgc tggcgcatct
    40261 tagcgaggat gatcgcaaac aggtgctaac gctgattgcc gatttccgca aagagctgga
    40321 taagcgcacc atcgggccgc gaggacgtca ggtgctcgac catctgatgc cgcatctgct
    40381 aagtgatgtc tgtgcgcgtg aagacgctgc cgttacgctg tcgcgcatta ccgccttgct
    40441 ggtggggatt gttacccgca ccacctattt agaattgctc agtgaattcc ccgcggcgct
    40501 taaacatttg atttctctgt gtgccgcgtc gccgatgatt gccagccagc tggcgcgtta
    40561 tccattattg ctggatgaat tgctcgatcc aaacaccctt taccagccga cggcgaccga
    40621 tgcctaccgc gatgagttgc gccagtattt gctgcgcgtg ccggaagatg acgaagagca
    40681 acagcttgag gcgctgcgtc agttcaaaca ggcgcagctg ttacgcatcg ccgcagcgga
    40741 tatcgccggt acgctaccgg tgatgaaagt gagcgatcac ttaacctggc tggcggaagc
    40801 catgatagat gccgtcgttc agcaggcgtg ggttcaaatg gttgcccgct acggtaagcc
    40861 gaatcacctg aacgaacgcg aagggcgtgg ttttgcggtg gtcggctacg gcaagctggg
    40921 cggctgggag ttaggctaca gttccgatct tgaccttatc ttcctccatg attgcccaat
    40981 ggatgcgatg actgacggtg agcgggaaat cgacgggcgg cagttttatc tgcgtctggc
    41041 gcaacgcatt atgcatctgt tcagtacgcg tacctcttcc ggcattttgt atgaagtgga
    41101 tgctcgactg cgtccgtccg gggcggcggg aatgctggtg acatccgcag aagcatttgc
    41161 cgattatcag aaaaacgagg cctggacgtg ggaacatcag gcgctggtgc gtgcgcgtgt
    41221 agtgtacggc gatccgcagc tcaccgcgca ctttgacgca gtgcgtcgcg agattatgac
    41281 gctgccgcgt gaaggtaaaa ctctgcaaac ggaagtgcgg gaaatgcgcg agaaaatgcg
    41341 cgctcatctc ggcaataaac atcgcgatcg ctttgatatc aaagctgatg aagggggaat
    41401 taccgatatc gaatttatta cccaatatct ggtgttgcgc tacgctcatg aaaaaccgaa
    41461 gttaacgcgc tggtcagaca acgtgcgtat tctggaacta ctggcgcaaa acgacattat
    41521 ggaagagcag gaagcgatgg cgctgacccg tgcttacact acgcttcgcg atgaacttca
    41581 tcatctggca ttacaggaat tgccgggcca tgtgtcggag gattgcttca ccgcagagcg
    41641 tgaactggtg cgggcaagct ggcagaagtg gctggtggaa gaatgaagta tggtattatc
    41701 gcgcgcaaat tttgaatctc tcaggagaca ggaatgaaag taacgctgcc agagtttgaa
    41761 cgtgcaggag tgatggtggt tggtgatgtg atgctggatc gttactggta cggccccacc
    41821 agtcgtatct cgccggaagc gccggtgccc gtggttaaag tgaataccat cgaagaacgt
    41881 ccgggcggcg cggctaacgt ggcgatgaat atcgcttctc tcggtgctaa tgcacgcctg
    41941 gtcgggttga cgggcattga cgatgcagcg cgcgcgctga gtaaatctct ggccgacgtc
    42001 aacgtcaaat gcgacttcgt ttctgtaccg acgcatccga ccattaccaa attacgggta
    42061 ctttcccgca accaacagct gatccgtctg gattttgaag aaggtttcga aggtgttgat
    42121 ccgcagccgc tgcacgagcg gattaatcag gcgctgagtt cgattggcgc gctggtgctt
    42181 tctgactacg ccaaaggtgc gctggcaagc gtacagcaga tgatccaact ggcgcgtaaa
    42241 gcgggtgttc cggtgctgat tgatccaaaa ggtaccgatt ttgagcgcta ccgcggcgct
    42301 acgctgttaa cgccgaatct ctcggaattt gaagctgttg tcggtaaatg taagaccgaa
    42361 gaagagattg ttgagcgcgg catgaaactg attgccgatt acgaactctc ggctctgtta
    42421 gtgacccgtt ccgaacaggg tatgtcgctg ctgcaaccgg gtaaagcgcc gctgcatatg
    42481 ccaacccaag cgcaggaagt gtatgacgtt accggtgcgg gcgacacggt gattggcgtc
    42541 ctggcggcaa cgctggcagc gggtaattcg ctggaagaag cctgcttctt tgccaatgcg
    42601 gcggctggcg tggtggtcgg caaactggga acctccacgg tttcgccgat cgagctggaa
    42661 aatgctgtac gtggacgtgc agatacaggc tttggcgtga tgaccgaaga ggaactgaag
    42721 ctggccgtag cggcagcgcg taaacgtggt gaaaaagtgg tgatgaccaa cggtgtcttt
    42781 gacatcctgc acgccgggca cgtctcttat ctggcaaatg cccgcaagct gggtgaccgc
    42841 ttgattgttg ccgtcaacag cgatgcctcc accaaacggc tgaaagggga ttcccgcccg
    42901 gtaaacccac tcgaacagcg tatgattgtg ctgggcgcac tggaagcggt cgactgggta
    42961 gtgtcgtttg aagaggacac gccgcagcgc ttgatcgccg ggatcttgcc agatctgctg
    43021 gtgaaaggcg gcgactataa accagaagag attgccggga gtaaagaagt ctgggccaac
    43081 ggtggcgaag tgttggtgct caactttgaa gacggttgct cgacgaccaa catcatcaag
    43141 aagatccaac aggataaaaa aggctaaccg gaaagcggtt cacagatctc gcttcgtaca
    43201 tggcaggcct attgacgctg ttgttttttc aacctagagt aaaggaacaa gggtaaggga
    43261 ggatttctcc cccctctgat gagttgttag taagtcggga aacttaacag taacaacaca
    43321 accagtatga tgacgagctt catcataacc ctttccttat acaaggcccc ttcttcggga
    43381 ggggctttcc cgtttcagcc ccttgctgcc ccccgattgt tgatgggcgc cggaccccgc
    43441 gcaatgcttt cccagaggca attttgcctt ccccgagcgg tcacgcaaaa cgctgcaacg
    43501 tcctgagcat ttgcaccttt tagcggaacg cagatataaa aaatccccgg ctttttgggc
    43561 cagggatatg tgttaatgaa accttcttga cgctgatttt ttttcaacct aaagtaaagg
    43621 aacaagggtg agggaggatt tctcccccct ctgattggct gttaataagc tgcgaaactt
    43681 acgagtaaca acacaatcag tatgatgacg agcttcatca taaccctttc cttctgtaag
    43741 gcccccttct tcgggagggg ctttcccgtt tcagccccct gctgactccc cgattgttga
    43801 tgagcgccag accccacgca atgctttctc tgatgcagtt ttgccttccc cgagccgtca
    43861 cgcaaaatgc tgcaacaacc tgatcatttg cactttttag cggaacgcag atataaaaaa
    43921 tccccggctt tttgggccag ggattattcc tggaagaggg tttactctgc tttttcttcg
    43981 acgggagttg ttgaggttaa gggtgaagtc aatgccgcca gtgagccgcc tgaaacgcca
    44041 atttcattga gcaaggagtc aatcagcggt gcctgtgtgc ggtaagagag agcggctgat
    44101 aatgcttgtt ccgccaggtt tccgcccccc acattacccg tgtttgcatc acccgcagcg
    44161 ccgccacgat tcagaccatc gacctgaata atcttgatac cgtcgattga cttcatcggc
    44221 tcaacggatt tctctattac cgcaggcagc gcctgcaaca aggccagttt gaatttaagg
    44281 ctggtttgtt catcagaaag tacgttgata gcatcgttca gcgcacgttg tgcttctgct
    44341 tccgccagac cctttttacg tgtagcttcg gctaactcaa cgatagccgc cgcctgcatc
    44401 tctgcggctt ctttttctgc tttcgcccgc acggtcagtt caaccgcttt ggtttctgca
    44461 tcctgcgcgg cagcgattag ggcaacttgt ttagcacgat cggcttcggc agtctggcga
    44521 gtggtttcta cgttttgttg ggcgcttacc gcttctgcaa gtgcgagatt agcacgcgct
    44581 tctgcctggg actgttgttc cgatttggcg gcaatagcga tcgatttcgt ctggttagcg
    44641 atttcggtga cctgctgctg ttcgatctct ttaatgcgaa cttcacgttc agcttcaacc
    44701 tttcttgagc ggacggcctg ttcgcggtcg atttctgttt cctgaatctg tcgttcagcc
    44761 agaattcgtg tctgctctgc ttcacgacga cgttcagctt caaaagccgc aatacgtgca
    44821 ttctgttcgg cagtacgggt tttaacctgc tgctcctgct caagcgtcat aaacgcttct
    44881 tgctgttcaa tctccagctt gcgcgatagc gcatcgcgat ttttttcacg caccgcaact
    44941 tctacatcct gttcaacttc gttacgttcg cggcgacggc gctctgtttc ctgagtcagt
    45001 ttggttaaac cttcggcgtc aaaggcattg ttcggattga aatgttcttt cgaggtctgg
    45061 ttaaagttgg taagtgaaac gctctccagt tccagaccgt ttttcgacag gtcttctgcc
    45121 actgtatttt gcaccccctg cacaaagttc tcgcgggtat cctgtaactc atgcatggtc
    45181 atttgcgcag ctgttgcacg gagggcatcg acaaatttat cttcaaccaa catacgtaag
    45241 tcttcaggcg acagggtgcg ttgccccagc gtctgggcag cggtggcaat cccttctact
    45301 gaaggtttta cccgcacaaa gaaagcgact actacatcga cgcgcatacg atctttcgta
    45361 atcaggctat caatggttga gcggctgact tccagcttca gagtattcat attgatgggg
    45421 attatttcat gaaagatcgg catcacgatt gcgccaccgc tcattaccac tttttgccca
    45481 cctaaaccag tacgaacaaa agcttgctct gccgaagcgc gacgatagag cctggcgaaa
    45541 ataattccaa taataaacag aatgcatacg gcaataattg cggtaaacat ccaggagggc
    45601 acagaattaa caatatcatc cattattaaa tttccttttg attaataaat tacaatattt
    45661 ggggccaggg attattttcc gccaaatatc gcgttgcaga aagtcggcaa ataattaaca
    45721 ctttgtctcc tttagtgaaa atttttcctt cttcaggctc cagtagtaaa taatgtattt
    45781 ggccaaattg atcggtgagt tttccttcgc agggattacc tgaagtggct tgatgaccgg
    45841 taattaacgc catgctacca atatattctt cttctgtaat agctgaacta tggtcgcgtg
    45901 gaatccaggg cgcaactatt ttaccggtat aatgcaccgc aataatcgta aacagtaaac
    45961 tgacaggaac cacgaacagg ttcgacagtg gtgattgcca gaccatgatg caggcatgct
    46021 gcaacaggat gccaataaga ccaaaaaaac cagccagtaa gcagagaacg acgagagctg
    46081 gcaatcttcc aatattgaga taatgaagtg cctggctaat atgacctgtg gttatagaat
    46141 cataatggtc aagatgtgca tcaagagcac cggagagcat gtgaccgcag ataagcgcaa
    46201 aaatttctaa caggccgatc aacagtacaa aggaaatagc gaaaagataa ggggtgttat
    46261 agtcggcgaa taaaatcata tatcactcca atgtccattt agtgacgcaa tatttacttt
    46321 gtaatagttt ccttgcttac atttatctgt gttaataata acacaggtta gagaagttct
    46381 ggaagagaaa gtcttttaat atggtttttt atttttcact aaaaagtgtg atcggggaca
    46441 atatatttac gcacgttatg tttaaaggca ctacactgat tggggaatac tgaaatcaga
    46501 aaaagcatat ggccttaaaa ggtatgcgat aaccggagga tgtgcttatg gatcatagtc
    46561 ttaattcttt aaataatttc gatttcctgg cgcgtagttt tgccagaatg cacgcagaag
    46621 gtcgcccggt cgatattctg gccgttactg gtaacatgga tgaagaacat agaacctggt
    46681 tttgcgcacg ttatgcctgg tattgtcaac agatgatgca ggcaagagag ctggagttag
    46741 agcactgata taacgggcct gatggcccgt tttagtgttt gtattagata ctagttatag
    46801 gtgattttca ggattacgtc tttactaaac ggtccctcaa caaccgtttc tgccgggttc
    46861 gcactcagtt ctgccctgaa tggtgcggtg accgaaggcg cactagacga tgaaaaagaa
    46921 gcaaattgtg agaagcggtt aaattccgtt gctgttccat cgttatgaaa tatcttcaat
    46981 agcagaccat tacctatatc cagatggcta taatcactca aaatatccgt agtgaaaaaa
    47041 ctccctaaaa tattaaactg ctcagtacag gccgcacccg gatctttggt catgactacg
    47101 ctaaacgtct cactcacgct ggtattttta attgttcggg aatttatttt accaaagtcg
    47161 acaacgccat cagctggttc cagatcaaca ttaacaaagc agtcaaggac atgaatgtta
    47221 tttaatccgc gaatcctgaa ggtcagattt ttatctactt ctatagcacc tgctccgctc
    47281 atacctttaa attcaagtgt tatatattcg tcaggtaata caagtttttt atttacttta
    47341 ttggcatttt tagctagctt aatatatagc tgcactcgta ctggaaaagt tactgataat
    47401 tgcggttcag gaccgccact acagactgcg ggctttttgt agccattccc gaaggttccg
    47461 gctcctttta tatcaataca tacaggtgct gcaataggta gtggagatgt agaatcataa
    47521 tcaaccccat tataagttac accgaaagta taagagtttt gattataaaa gtcttgacct
    47581 gatgctaaca acatcattat atttgcccat gcagatgggg attcttctcc tggagatggg
    47641 cctggtagtg cattatcaca gaaaacattc ataaaatatt cactgctttg ccagatttta
    47701 tcaccttttt taccatcgct ggggatagag attcttggta cgtaaccaat gacaacggta
    47761 tttccacctt catgttcagc atagcagtcc attgcccatg caggtaatga actaaaaccc
    47821 ataaaaaaag taataacaat taacaagtag cgcatattat tgatctcggt tggctacaga
    47881 aatattacaa atgttattta ctacagggca gtttatttca taaaaactta atccaccaaa
    47941 gtcattgata taacccacct gaaaagttga cggaacattt ttaaactgcg tatttgcata
    48001 gctaaaaggg gttaccatta agctttgcgt ttgtggtaac atcgttaccc catttgttcc
    48061 gatataaccc agcgtaacgt gataaggcat cttattgtta aagcggatcg aaccgtcatt
    48121 tctacgaatg atttcaactt tttcgtaagt cattacccca tcgtcttcta attcaatggc
    48181 ttttggccgc cagaatagtt tcaatgcgtg ctgcatagtg acttgcatca catttttttt
    48241 atctgttttg ggcggtattt ctcgcacgtt aaaatagaag agcgtttctc tgtctttagg
    48301 aagttgagca attttttcct gagttatcat tcttatttga atttgctcac tgggatttaa
    48361 acgcaccatt ggaggaacag tgacaataaa gtcacgcgat ttattgcctt tcgtatcttc
    48421 tatccatgtt tgggccagat agggcaggtt tttactttga ttagtaagtt ttaatgcttc
    48481 gcctttatct gactcattca ttatgatacg ggtacgatcc gggatgatgg caccccaggc
    48541 aatgctgctc atacaggaga gtaatactcc tgctgcaatg atattctttg tatttaagta
    48601 gcgcattagt ttcatcctga attaattttg gctaatacac ggtaaaatag ccgttccggt
    48661 ggttaagttg ctgctattgg gtggttgaat aaaacaagat ttgtcacccc agcgtaaagc
    48721 aagtttgtta tcatcctgaa tgccgttgag gtatacaaag ccgccatcag caaccagtcc
    48781 taattctttg tggctggttt catcttttac gctaatcccc agtggtggat gactaccatc
    48841 tgccaggcga ataaccccta tgatttggta gcccttgcgg gtatcaagtt tggcgtagcc
    48901 aatagcaccc tcggttaagg tggtggtgat aaccgagttt tcgatatcca cattttctgg
    48961 cagatttcgg gtatcaacac ttaatgatgt tgttatgtaa ctgctgactg atggaataac
    49021 tccgatgcca taccgattag ttaccgcgcg tttattgttc aacggaatat cttcagtgcc
    49081 gtctgcgtcg atcataaaac gcgagtcagc actaccgcta tagtcgtgga aagcggcacc
    49141 gtggcgagtc gcagtgaatg aaccattcca actggcattc agagaataat aactgtctcg
    49201 ctgtgatgta ccggagaggt agagtcgacc gttctcggta ttatgctgat aactggcgcg
    49261 tagtgacgct ttcatatctt tgaattcatc attgtcgcct gaagcagaaa cgctccagga
    49321 attatttcga tcggaagagt cataccacga agcagtatgc gaaatctcat tatcctgatt
    49381 tcgttgcata ccatagctca atgttctact agttccccag ggaatcgaaa tatttaggta
    49441 cagttgatcc tggttgtctt cttcccagtt tattcggcta aatgttagag aagtggagac
    49501 gttttttaat ggcccgatat caaaatcgcg gctaagtgat aatgaataat tcacattact
    49561 gctggcgtcc cagtaagtat tacgagccaa acttacgtat gtatttaacc ttaatgattc
    49621 aagatactgg ttaaacgtga caatataact ctctttttca ttctgataat tcgtgtaatg
    49681 gttagtgtca ttgatatatt cattcattgt cacaaagttt ttatcagaga aacggtaacc
    49741 agcgaaagtg agctggctac cggtagattc aaaacgtttt gaatagttag cgcggtagct
    49801 ataacccgtt tctttatcct gattatgcaa ctgagcatca gatcgtgtga catcaaaaga
    49861 taatgaacct aatgagttaa ggttaaaacc aacgccggca gccagagatt gataatcacg
    49921 gtttgttaaa actgaaccac catacaggga tacattgttc agccaccccc atgagaattc
    49981 accagtccag aagaagggtt gttgtaagga atcaccacca acgctggtcg gtttacccat
    50041 agcagtggtg tagcgaatct gccctttacg cgtcagataa ggaatactat tagatgcaac
    50101 ttgccattgc gtaacgcgtc cgtcctcttc ttcaaccttg acgtcaagtt gcccctgtaa
    50161 tgtattgaat aaattggtaa tagcaaatgg gcctggagga acattttctt gataaataat
    50221 acggttgttc tgtgagacag tgacctttgc attcgtttgt gcgacacccg tgatttgcgg
    50281 tgcgtaacca cgtaggtctg gcggtaacat attctcatcg cttttcaacg aaatgccaga
    50341 aaaatggaaa gagtcgaaaa tagaggattc agtatcgtat tgacctatag ttagttttgc
    50401 attcattgct gggattgggc gaaacaaata aatacgcgga aaatccaggt tggtcgcctg
    50461 gcttttgcca ttgttaaatg actgatcgta ctggtaatca gtacgcaggc gccaggcccc
    50521 aagattaacc ccagcctgcc cataggaact tatattttgc gaattatcgc cctgatgtgg
    50581 tgcataatga gaggcatata aattgtagtc aagaaatgcc ccagcaatac catgattcca
    50641 aagttcagga ggcgtccagt ttgcatcttt gtattttaac catgcctgag gtgcagatat
    50701 ggataattgc attttccctt tatcgagata agttgtaatt tgtttttctt tttcgatagg
    50761 gtaacaacca tcgatcaggt tttgctgagt tttttcaata aattcatcag taaatcctaa
    50821 taatgttaat tgctctggtg tcacgcaaac tgcagtttgg ttatcaatta atacccattg
    50881 gaggtcaagg tggattggcg tcctcttatt attaatcaga gtgcttagtg aatatttgcc
    50941 gacgggtaat tggccatcag tttcaaattg ggaaaggtta acgttttcac gatcttcggc
    51001 ttcgattaaa tctttgttga attcaacggc ataagctgac gcacaaccaa tgatgataac
    51061 ggataatgaa caataaatat ttttaatcaa ttcgcttatt gtggttaatt ttaatttttt
    51121 atacatctga tccacttatc attaatccgt taatgatgtg gccgggcgaa cccggccaaa
    51181 tgcgattact cgtaagcaat cttgtagtca gcctgagcgt aaacgttgcc cggggtggca
    51241 tctttgcttg gggattccat acgagctttg aaattcagtt cctgatagct gttggtattg
    51301 ttgaacgtaa ttactaccgg agtacctaaa gttacttcgc cgctctggct gtccagaata
    51361 cgtacgccaa cgccagtggc gccgccgatt tcagtttctt tggtattttt cagaagatca
    51421 gactgacccg tcatggtatt tgctgtagtg aaagtaacct tcgcttttga ataagtagtt
    51481 ccaccctgac tcagcatgca gtcctgcagg tgaatagtga aaggttcagc caaagatttc
    51541 tgatcgcttt tcagtacggt atctgctact tcacccagat ttacagttaa atcttcgtca
    51601 cctggtttaa tagaacatgg agcagagata acttcacctt taaatttaac aataccgtga
    51661 ccttcatcag caaacacgtt attggaaaga atcattgcta cgccagcaac aagaagtgac
    51721 tttttaaaag cggacataat attcctttat ataaagataa gtagaaaaga gaatgattag
    51781 taactgttat ttaaggactg ctaatcattg aaatatgata ttttattttg tgttttttat
    51841 aaaattagtt tatctaaata tcgattaagt aaaacttaaa aaatacattg caattatact
    51901 ttcaattgta ctcattcatt tttgtgatgg ttttctgaat gggattacgc gataaggtaa
    51961 tagaaagcac tataaattag aggattaatt cataatttac agtgcattaa agacgaatgt
    52021 ttacagcgtt ggggggagag tctctggatc tggctgcttt ttgatctcag tactgcgatt
    52081 ttccagttcg ctgattcttt gttcaagcag cgccagtttt tcacgagtcc gtaacaggac
    52141 ttgcgtttgg acgtcgaact cttcacggct tacaaggtcg aggcgcgtca gctgcgcttg
    52201 tagggtttgg cggatttttt tctccacatc ttccccgaac tccctgattc ctttaggcat
    52261 tgattcgtga acctggcgag cgatttgctc aatttttttc gggtcaatca ttgtagtttc
    52321 cctgtactga taggtgttga gtgccattgt agtgcgataa gggtaagtca taaaccagaa
    52381 ttatgtgaag ctatgcgttg ctgccgctaa tcattagcgt tatagtgaat ccgcttattc
    52441 tcagggcggg gcgaaattcc ccaccggcgg taaatcaact cagttgaaag cccgcgagcg
    52501 ctttgggtgc gaactcaaag gacagcagat ccggtgtaat tccggggccg acggttagag
    52561 tccggatggg agagagtaac gattctgtcg ggcatggacc cgctcacgtt attttggcta
    52621 tatgccgcca ctcctaagac tgccctgatt ctggtaacca taattttagt gaggtttttt
    52681 taccatgaat cagacgctac tttcctcttt tggtacgcct ttcgaacgtg ttgaaaatgc
    52741 actggctgcg ctgcgtgaag gacgcggtgt aatggtgctt gatgatgaag accgtgaaaa
    52801 cgaaggtgat atgatcttcc cggcagaaac catgactgtt gagcagatgg cgctgaccat
    52861 tcgccacggt agcggtattg tttgcctgtg cattactgaa gatcgccgta aacaactcga
    52921 tctgccaatg atggtagaaa ataacaccag cgcctatggc accggtttta ccgtgaccat
    52981 tgaagcagct gaaggtgtga ctaccggtgt ttctgccgct gaccgtatta cgaccgttcg
    53041 cgcagcgatt gccgatggcg caaaaccgtc agatctgaat cgtcctggcc acgttttccc
    53101 acttcgcgct caggcaggtg gtgtactgac gcgtggcggt catactgaag caactattga
    53161 tctgatgacg ctggcaggct ttaaaccggc tggtgtactg tgtgagctga ctaatgacga
    53221 tggcacgatg gcgcgtgcac cagagtgtat tgagtttgcc aataaacaca atatggcgct
    53281 cgtgactatt gaagacctgg tggcataccg tcaggcacat gagcgtaaag ccagctgaaa
    53341 accgctgctt aatttactgc cttaatcaag aaaccgaagt tgtagcaggc ttcggttttt
    53401 atttttccct gctatgcaag atgttaattc cgcttctctt tctatgagaa aatttcatta
    53461 atatcaggca ttctttttca ttatgatcaa aatcatataa ttgttccgtt attatgcgac
    53521 catatgaata tatccgaaaa aggatatgtg aaatttatac gctaaatgtt tagtattgtt
    53581 aaatattaag ggtaaaagga tgtttatcgc ctggtactgg attgtattga ttgctctggt
    53641 tgtggtgggt tatttcctgc atttgaaacg ttattgtcgg gcgtttcgcc aggacagaga
    53701 cgcactgctt gaagcgcgga acaaatactt aaacagtacg agagaagaga cagccgaaaa
    53761 ggtagaataa ggctttcgtg aagctggcag aagttgtcca tgcgctggac aacttctgat
    53821 gttgttttta accaattccc gccgtttgta gcagcactaa actgaatccc atcactgaca
    53881 ttccacatag tacgccatag ctggggttat tattagggtc aatctctttg gcgagcggca
    53941 ttaattcatc aaccgagagc gccaccataa ttcctgcaac cgccgccatg attgccgcca
    54001 tgaccaccgg ggaaatcatg ctaccgagga ttaaccacgc cagcacacca ccaagaattt
    54061 ctgctagtcc agaaattccc gcccacagaa tcgcggtgcg tttagacccc gttgccgcat
    54121 aaaccgggcc tgccactgcc agaccttcag ggatattgtg caacgcgacg gccagtgcga
    54181 tgccaaatcc cagctccagg ttgctgctcg ccgtgacaaa ggtggcaatc ccttccggga
    54241 agttatgcag actgatgccg agagtgagca gaatggctgt gcgcttgatc gattttggca
    54301 acggctgcac cgatttttgc attaaatcct gcggatgagc atgtggcaac atgcggtcca
    54361 ggccaaaata gccaagcaga ccgaagataa acattccata acccaacaca ggcgacattc
    54421 cttcagctgc tagtgcggca ggaagcattt ccattaatga gatgagcaac atgatccccg
    54481 ccgcaaaacc tagcgaaaac gccagtaagc ggttcgaggg tttttgcccg agaacgccga
    54541 gaaacgcgcc aataaacgtg gctgcccccg ccagtatggt cagaatgaga ggtactgaca
    54601 tcgacaactc cttataaatc ttcaccttca ggcagatcgt caaaataact gatgatcatc
    54661 atcattgtta tccgggttct tactccgccg aacaagcgta ttgtgaggat atcaaaatga
    54721 cacctttagt taaggatatc atcatgtctt caacacgtat gccagcattg tttttaggtc
    54781 acggtagtcc gatgaacgtg ctggaagata atttgtatac ccgcagctgg cagaagttgg
    54841 ggatgacatt gccacgcccg caagcgattg tggtggtttc ggctcactgg tttacccgtg
    54901 gaacaggagt gaccgcgatg gagacgccgc ccacgattca tgactttggt ggcttcccgc
    54961 aggcgctgta cgatacgcat tatcctgctc cgggttcgcc tgcgctggca cagcgtctgg
    55021 ttgagctgtt agcgccgatc ccggtgacgc tggataaaga agcctggggc tttgaccacg
    55081 gctcgtgggg cgtgctgatt aagatgtatc ctgacgctga tatcccgatg gtgcagttga
    55141 gtatcgacag tagcaaacct gccgcctggc atttcgaaat ggggcgcaaa ctggcagcgc
    55201 tgcgagatga aggaataatg ttggtcgcca gcggtaacgt ggtgcataac ctgcgcacag
    55261 tgaagtggca cggtgatagt tcaccgtatc cgtgggcgac gtcgtttaat gagtatgtga
    55321 aagcgaatct gacgtggcaa gggccagtgg aacaacatcc tctggtgaat tacctcgacc
    55381 atgaaggtgg cacgttatcg aacccaacgc cagagcatta tttgccgttg ttgtatgtgt
    55441 taggtgcgtg ggatgggcag gagccaatta ccattccggt tgagggtata gaaatgggca
    55501 gcctgagtat gctgtcggtg cagataggct aacaattacc ggatggcgag tgccatccgg
    55561 tatcgtggct tattcaacaa aaatatgtgg ataaaaccga gacatatcct gggtgatcaa
    55621 tgcacggtct tcacgaatgc caattccggc gggttgatcg ttcaccagcc agctaccaat
    55681 cagcatatag ctgtcgccga atttcggtaa cgggtggaat tgctgaacaa tcatcccttc
    55741 ttcgccatac ggaccttccg ctgcttcaat ggttttgccg ttctcaatga tcgacacgtt
    55801 tgcgccttca cgggagaaga tcggtttaac cacatatttt tccatttgcg gatgatcatc
    55861 ttccgcaaaa taagcgggca gcaggttcgg gtgattcggg aacatctccc acagtagcgg
    55921 tagaagtgcc ttgttggaga taatgctctt ccacgccggt tccagccagc gtacgcctgc
    55981 atcctccagc ttggttgaga acatctcacg caacataaat tcccacggat acagtttgaa
    56041 caggttggaa attacctgat cctgtaaatc cgtgaactga cctttttcac ctaacccgat
    56101 atcatcgatg tagaggaact cagtagcaat ttcagcttcc gttgcgcagt cctgcaaata
    56161 ctgaatggtt ccgcgatctt ccaccgtgtc gcgacagcag gtgagatgca gcaactggaa
    56221 gccatactgt tcacgcagct caacgaagcg atcgatcagt ttttcttgca gactgttaaa
    56281 ctggtcgctg ccctccggca agttaccggc gttaagctga tcttccagcc agatccactg
    56341 aaagaacgcc gcctcgtata gtgacgttgg cgtatcggcg ttattttcca gaagtttagg
    56401 ttcaccagtg ccatcccacg ccagatcaag acgcgaataa agcgatggct ggtgcgtcag
    56461 ccatgactgg cgcacaaaac tccaggtgtg ttttggaatg cggaatttgg tcatcagctc
    56521 atcgctggcg atcacttttt ccaccacttt caggcacatc tggtgcagtt cggcggtgac
    56581 ttcttccagc ttttcaacct gggcgagggt caacttgtag taagcatctt cacaccagta
    56641 cggctcgccg tacatggtgt gaaaattgaa accgtattcg tgggctttct cacgccagtc
    56701 cgggcgctcg gtaatactga ctctttccat cggtatcagc cacccattga acgagaagag
    56761 gtaccggttg cactacgctg catagtgctt tgtttggcaa cagattcacc aaaaccgcca
    56821 cgggtaacgg tagtggtggt cgccggtttt ggtgccattg ccgtcttcgg tacggtcatg
    56881 gtgcggcctg gctgggctgc gccatagttt ttacccgtcg cgtcggtata tttaccgtaa
    56941 gccggactgg ctgggttttt cgaggagaac agcggctgct gtgcaaatcc cgcgccgccg
    57001 cccatcagac gccccatcat gtaaccggcc atcagcggca tccagaaact cccgctggat
    57061 tgctgggcct gcgcctggtt ttctggtgcc atgccagcct gggctggtgc ctgctggcac
    57121 tgaccttcac caaattcagc aacacagtct tcacgggtgg cgtatttcgg cgcagtacgt
    57181 tcggcttctt tcagcgcatt gttgtacgcg gtggtacatt cggcgctttt gcctgggttt
    57241 gcagctgaac agtcgtcagc attttgatag agagacactg tttcatcact cttttcacag
    57301 ccagccagca taaaaacagt ggcaaccgcg agagcgactg gtgtcagatg gcgtgcgctc
    57361 cagtttttgc ggaacgatgc gtggcgtatg gattttgtcc gtttcatttt tgtcttccgg
    57421 gaccagtggt aaatacccat cagaatagag gatggctggt cgaaattgaa gcgagaaggg
    57481 ggaagaatgc ggcagataac ccgtatcttt acgttgcctt acgttcagac ggggccgaag
    57541 ccccgtcgtc gtcatcagtt acggaaaggg ttatgaccgt tactggtggt agtgcgtgcg
    57601 gatgtttgct gaacgactgg tgccgggcta tcaggcgcat aaccatcagc aatagcattc
    57661 tgttccggcg tttgcggtgc aacgttttcc ggattagtgg aaaccggttt gctcagcgca
    57721 ttgttcagtg ccagcagatc ctgctcgttc aacgtaccca gagctgactt aatattcagc
    57781 tgattaatca ggtagttata acgcgcattc gccagctctt gcttggcgtt gtacaacgtg
    57841 gtggtcgcat ccaacacatc aacaatggta cgcgtaccga ccgagtagcc cgcttccatc
    57901 gcgtctaatg agctttgagc ggaaactacg gcttgtttgt aggcgttaat gctactgata
    57961 gatgcattaa tgttgttgaa ggaggaacgc acggtctgca cgacgctacg atgggcactt
    58021 tccagttgct cgctggcacc gacaaagttg tactgtgcct gtttcacctg cgagttaacc
    58081 attccgccct gataaatcgg cagcgagaag ctcaggccaa ctttgttctg gcccatattg
    58141 ctatcgtcat actgggtacc agcggcacca cgggttttcg aaccgctata agaggtgtca
    58201 gaaatcccgg tagaagccgt taaatccaga gtcggtaagt gaccatcctg cgcctggcga
    58261 atttgctcgc gcgccaggtc ctggctcaag cgtgcctgta acagcgacag gttgcgtttt
    58321 tcggcttctt tcagcagcgc gttaaccggc tgtggtttgt cggttttaaa gttttcgaca
    58381 ttcagcgcag ccagttccgg atagtagtta ccggtgatct ggcgcagctg ctctaccgcg
    58441 ttatcaaggt tattacgtgc ggtcacttcg ttcgccagca cggtatcgta ctgtgcgcgg
    58501 gcgttctgca cgtcggtgat cgctaccagg cccacgttaa aacgttgggt ggtttgatct
    58561 aattgacggt agatcgcttc tttttgtgcc tgtgtatagg aaagaacgtc aatagcattc
    58621 aacacgttga aataagcggt cgcggtgttg aggatcaagg tttgctgatc ggtctgatac
    58681 gtgacgtcct gaatccctgc tgctttttcc tgcagcgtta acgcacgcca tttcgacata
    58741 tcaaaaatgg attgagttaa ctgcaaggac gcactggtcg cgttagagtt gatgccgttc
    58801 gcgtcgcggt agccgttgct ataggtgtaa tctgcaccta aacctagctg tggcagtaat
    58861 ggactgcgcg cttcattaat tttttcaaag gcagcatcac gatcggcggc agacttacgc
    58921 aattccgggt tactaaggcg tgcttgctga taaacttgca tcaggttctc ggcctggctc
    58981 aacgaactga acccagaaag gctcaggccg ataagaatgg ggagcaattt cttcatttgc
    59041 attccttgtg gtgaagcagt atttagcgcg atcaaactgt aaaattattg cggattctag
    59101 cagaagccgc taccgcaaaa ggttggcgtt acgtgccata gggcgttaat gtgcctcaat
    59161 ttaacacatg actgctcaaa cggcagtcaa acgtcgctga aattcacatt taattcacta
    59221 ttagtgccag gacattaaca atgcttaagc cagacaacct gcccgttaca tttggcaaaa
    59281 acgatgtaga aattattgca cgagaaacac tttatcgcgg ctttttttca ttagatcttt
    59341 atagatttcg tcatcgtcta ttcaacgggc aaatgagtca tgaggtgcgg cgggaaattt
    59401 ttgagcgcgg tcacgccgca gtcttgctac cctttgaccc agtgcgtgat gaagttgtgc
    59461 tgattgagca gattcggatt gccgcgtacg acaccagcga aaccccttgg ctactggaga
    59521 tggttgccgg gatgattgaa gagggtgaaa gtgtggaaga tgttgcccgt cgcgaagcga
    59581 ttgaagaggc gggactgata gtcaaacgga ccaaaccggt gttaagtttc ctggcaagcc
    59641 cggggggcac cagtgagcgt tcgtcaatta tggtgggcga agtggacgcc acgaccgcaa
    59701 gcggtattca cggtctggct gatgaaaacg aagatattcg cgttcatgtg gtaagccggg
    59761 aacaggcata ccagtgggta gaagagggga aaatcgacaa cgcagcgtcg gtcatcgctt
    59821 tgcaatggct gcagctgcat catcaagcgt taaaaaatga gtgggcataa atgaagcgtt
    59881 acacacctga ctttcctgaa atgatgcgcc tgtgcgagat gaacttttca caattgcgcc
    59941 gtttgttacc gcgcaatgac gcacccggcg aaactgtaag ctatcaggtg gcaaacgcac
    60001 aatatcggct gacgattgtg gaatcgaccc gatacactac cctggtgaca atagaacaga
    60061 ctgcgcccgc gatcagttac tggagccttc cgtcaatgac ggtgcgtctg tatcatgacg
    60121 cgatggtggc tgaagtgtgt tcaagccagc agatttttcg cttcaaagcg cggtatgatt
    60181 atcctaataa aaagttgcat caacgcgacg aaaagcatca aattaatcag tttttagcgg
    60241 actggttgcg atactgttta gcacatggag cgatggcgat tccggtttat tagcgtcgtg
    60301 aaacctaagg acaccatttg gaaagcctgt taacccttcc tctggctggt gaggccagag
    60361 tcaggatttt acaaattacc gacactcacc tgtttgcaca aaagcacgaa gccctgttag
    60421 gggtaaacac ctgggagagt taccaggcgg tgctggaggc gattcggcca caccagcacg
    60481 aattcgacct gattgtcgcg acaggtgatt tagcgcagga tcaatcctct gcggcctatc
    60541 agcatttcgc tgaaggcatc gcaagttttc gtgcgccctg cgtctggctg ccgggcaacc
    60601 acgatttcca gcccgcgatg tacagcgcgt tacaggatgc gggtatctcc ccggcgaagc
    60661 gcgtgtttat tggtgagcaa tggcaaatcc tgttgctgga tagccaggtg tttggcgtgc
    60721 cgcacggtga gctgagcgag tttcagcttg agtggctgga acgtaaactg gccgatgcgc
    60781 cagaacgcca tacgttgctg ctgctgcatc atcatccgct acctgcgggt tgtagttggc
    60841 tcgatcaaca cagtctgcgt aacgcgggcg aactggatac cgtgctggcg aagtttccgc
    60901 acgtcaaata cttgctgtgc ggtcatattc atcaggagct ggatctcgac tggaatggtc
    60961 gccgcctgct ggcaacgccg tcgacctgtg tgcagtttaa gccgcactgt tccaacttta
    61021 cgctggatac catcgcgccc ggctggcgta ctctcgagtt acatgctgat ggcacgctga
    61081 ccaccgaggt gcatcgcctg gcggacacac gtttccaacc tgataccgct tcagaaggct
    61141 actgatgtct acgcttcttt atttacacgg tttcaacagc tcgccgcgct ctgcaaaagc
    61201 gagcttgtta aaaaactggc tggcggaaca tcaccctgac gttgagatga tcattccgca
    61261 gttgccgccg tatccttccg acgcggcaga gctgctggaa tccattgtcc tggaacatgg
    61321 cggtgattcg ctgggtattg tcggttcgtc actgggggga tattacgcca cctggttgtc
    61381 acaatgtttt atgctgcccg cagtggtggt aaacccggcg gtgcgcccgt ttgaactgct
    61441 gacggactat ctcggtcaga acgagaaccc ctacaccggg cagcaatatg tgctagagtc
    61501 acgccatatt tacgatctta aagtcatgca gattgacccg ctggaagcgc cggatttgat
    61561 ctggctgctg caacagacgg gagatgaagt gctggattac cgccaggcgg tggcgtacta
    61621 cgcttcctgc cgccagactg tcatagaagg cggcaaccac gcattcacgg gcttcgaaga
    61681 ttatttcaac ccgatcgtcg attttcttgg tctgcaccat ctctgacgat caactcgaat
    61741 tactaactta aaccatgacg caaacttata acgctgatgc cattgaggta ctcaccgggc
    61801 ttgagccggt tcgccgccgt ccggggatgt ataccgatac cactcgccct aaccatttgg
    61861 ggcaagaagt cattgataac agtgtggatg aagcactggc gggtcacgca aaacgcgtgg
    61921 acgttatttt acatgctgac cagtcgttag aagttattga cgatgggcgc gggatgccgg
    61981 tggatattca cccggaagag ggtgtaccgg cggttgaact gattctttgc cgtctgcatg
    62041 caggcggtaa attctctaac aaaaattacc agttctctgg cggcctgcat ggcgtgggga
    62101 tttcggtggt taacgccctg tcgaagcgcg tagaagttaa cgtgcgccgc gatggtcagg
    62161 tttataacat cgcctttgaa aatggcgaaa aggtgcagga tttacaggtt gtcggcactt
    62221 gcggtaaacg caatactggt accagtgtgc acttctggcc ggatgaaacc ttctttgaca
    62281 gcccgcgatt ttctgtttca cgcctgacgc atgtgctgaa agccaaagcg gtattgtgcc
    62341 ctggcgttga gatcactttt aaagatgaga tcaacaatac cgaacaacgc tggtgctatc
    62401 aggacggtct gaatgattac ctggcggaag cggtaaatgg tctgccgacg ctgccggaaa
    62461 aaccgtttat cggtaatttc gctggtgata ctgaagctgt ggactgggcg ctactgtggc
    62521 tgccggaagg cggtgaactg ctgaccgaaa gctacgtcaa ccttatccca acgatgcagg
    62581 gcggtaccca tgttaatggt ctgcgtcagg gcctgttgga cgcgatgcgt gagttctgtg
    62641 aataccgcaa tattctgccg cgcggtgtaa agctgtcggc ggaagatatc tgggatcgct
    62701 gcgcctatgt gctgtcagta aaaatgcagg atccgcagtt tgccgggcag acgaaagagc
    62761 gtctctcttc gcgtcaatgc gcggcattcg tttctggcgt ggtgaaagat gcctttatcc
    62821 tgtggctgaa ccagaacgtt caggcggctg aactgctggc ggagatggcg atttccagcg
    62881 cccagcgccg tatgcgtgcg gccaaaaaag tggtgcgtaa aaagctgacc agcggcccgg
    62941 cgttgcctgg caaactggct gattgtaccg cgcaggacct taaccgtacc gagctgttcc
    63001 ttgtggaagg tgactccgca ggcggatctg ccaagcaggc gcgcgatcgc gaatatcagg
    63061 cgatcatgcc actgaaaggt aagatcctta acacctggga agtctcttcc gacgaagtgc
    63121 tggcttcgca ggaagtgcac gatatttcgg tagcgatcgg tatcgatcct gacagcgacg
    63181 atctgagcca gcttcgttat ggcaaaatct gtatcctcgc ggatgcggac tctgatggtc
    63241 tgcacattgc cacgctgctc tgcgctttgt tcgtaaaaca tttccgcgcg ttggtgaaac
    63301 acggtcacgt ttacgtcgca ctgccaccgc tctaccgtat tgatctcggg aaagaggttt
    63361 attacgcgct gacggaagaa gagaaagagg gcgtacttga gcaattaaaa cgcaagaaag
    63421 gcaagccgaa cgtccagcgt tttaaaggtc tgggggaaat gaacccgatg caattgcgcg
    63481 aaaccacgct tgatccgaac actcgccgtc tggtgcagtt gactatcgat gatgaagacg
    63541 atcagcgtac tgacgcgatg atggatatgc tgctggcgaa gaaacgctcg gaagatcgcc
    63601 gcaactggtt gcaagagaaa ggcgacatgg cggagattga ggtttaaagg aaagaacatt
    63661 gcccggtcag gatggccggg caaacaaggc aggattaaat ccctggctgc agaatacgga
    63721 tattcatctc cagcacgtca ccttttacgg cttcgctata cgccttcatg tgcggggttt
    63781 gcagatgcgc ttcaagatgc gcgatgcttt cccactgctc aatcatcacg atagaatccg
    63841 gtgccataga ctggaaactc acgccagctg cgcaatccac cattggcgca tagccgtggc
    63901 aaccttcttc tttcagtacg gttggaacga ttttagcaaa ctgatccaat accgcctgac
    63961 ggtgatgttg accaggacga gtacggattt ctgcgattac ggtaagcatg gttaactcct
    64021 tctaaagcct gagctctagt taaccaaaaa tttccacaag atgcttgcga tattcttcag
    64081 tatagcgggg aacatcaggc atttttatca cgtcattagc gataaatgtc ggcagcggtt
    64141 ccataccgag gaattggttt gctttatgga acggcagata cacaccgtca acgccaacgc
    64201 cgtggaagaa ctgatctttt tcggtgaagg cttccattgg tgcgttccag gtcagagaaa
    64261 gcatatattt tttgccctgt accaggccgc cggaaccgta ttttttcgac ggatctttgc
    64321 gggtacgacc atcgctggca tacagcgtcc cgtgaccttc ggtgaataca tcatcaatgt
    64381 attttttcac tgtccacggc gcgcccatcc accagcctgg catctgccag atcaccacat
    64441 cagcccagag aaagttttgt acttccgctt tgacatcgta gtcgctgtcg gcgcgaacga
    64501 tgcggacatc atgcccaagg tcgcgcagtg tgccatccgc gacttcggtc agggtgtcgt
    64561 tcagttgacc attggagtgg gcgaattttt tcgcgccgtt gataatcagg atgttgctca
    64621 ttttttaacc tcaaggtgag acgtttgccg actatagtac gtcacagagc ggtacggtga
    64681 aattagcaaa atgtgcaaag tcttttgcga atttagcaaa agtctgatct tactctgact
    64741 caccgatgca ggataaatat tttctgaaaa tgacaatact gatagccatt cctcataatt
    64801 cttactcgca ccggaataat ttacacgttt ggttcggttt ttttggggat tgaaaatatg
    64861 gctttgaata agtaaaagat gaaagtatca acgtgtcatg ttgataatta taaatggatt
    64921 tttatgagcg tactgatata tgttaaagca gaaaattaaa acaatatttg aagcattgct
    64981 atatataatg cttacttatt ggttaataga ttcttttttt gctttcaata agtatgactg
    65041 gatgcttgag tcgggaggaa atatttgcag tataccttcc gtttcaggcg aagatcgtat
    65101 attgcaagca atgattgcag cattttttct tcttacgcca cttattgttc ttatcttaag
    65161 aaaactcttt atgcgggaaa tgttcgagtt ttgggtatat gtattctcgc tcggtatttg
    65221 tctggtttgt ggttggtggc ttttttgggg acggtttata ttttgctatt agtaaaatct
    65281 atgagagtcg ttttaacggc tctcatagac agagaagtta ccagcttacc ttcgcctcaa
    65341 atccaccttg ttccgcattc ccaaattcaa cattcatgcc atgcaatttg gcgattcgct
    65401 ggacaatcga tagcccaagc ccgctgccgg tagcggtttg tccgggtggg cgatagaagc
    65461 gttcgccaat tcgcgccagt gcctctggtg tcacaccggg gccgttatcc ctcacgatga
    65521 aattatcagc attcagcgtg acgtctacca cgctgccctg tggactgtag cgcacggcgt
    65581 tatccagcaa atttcgcacc aacaaactta gcaatagcgg ttgcccggtg cgtttgatgc
    65641 tgtgggcatt gagtgtcagt cgcacgtcaa ttttcgcctg ctgcgccgtg tggtaaatat
    65701 ccatcaccga cgattgcagg agatcttcaa gcgggatctc cgcgacgtcc tgaaggttat
    65761 ccagtgagtc cagccgcgat agcgtgagca gttgatcaac cagacgagta gcgcgatcga
    65821 tcccggaatg taattggagc agtgcttttt tccgcgcctg cggatcatcg tcagagagct
    65881 gcgcaacttc ggtttgcact ttcagcgccg ttaacgggct acgaagttcg tgagctgcgt
    65941 cggaggtaaa gcgtcgttca cgaaccatca tcgcatgtgt gcgggcgaac agttgattta
    66001 gcgactcaac cagtggccgc acttcgctgg gtacgccagt cgcgtttagt ggtttttccg
    66061 agtcagggtc acgcatacgt agtgccagcg ccagtttgtt cagcggcgcg agttcacgac
    66121 ccagtagtac catcatgatg attaacataa tcggcagtgc gaccagccac gggatcaatt
    66181 gcccggcaac aatcgccagc gccatgtctt cacggtattc ccattcctgg ccaacaacga
    66241 tgcgatattt gccatcaggt gaggtcatcc agacaaaacg ccaaggatcg tcttcaccga
    66301 ccagttgccc gtcagcaaaa ccttcccgtt gatagctata gggaatatct tctccgttat
    66361 cgccatcatt aaggaccatt ctgccgtcgt gggtaaagat ggcaaaggtc agcgcatcgt
    66421 catcaacatg accgtgtttt aatctatttg gcgtctgtgc catgcgatcc gccgcgttga
    66481 tttcgttgag atcgagcgta cttaaccgct tggcaaacag catcagttgg gtgtcgaaca
    66541 attcatcgac gttatccgtt gtttgtttcc aggcgacaaa gctggaaagc agccaggtca
    66601 ccgaggccag aattaaaaag attagcgtca gcctgacgcg cagactaaga cgttgggtaa
    66661 atttcatttc tcacctaatg tgtaaccaat accatgcacg gtacgaataa aatcactacc
    66721 gagtttgcgt cgcagatgat gcacatgcac ttcaacggca ttactggtga cctcttcgtc
    66781 ccaggtatac agtttctctt caatcagttt gcgcggcagt acccgaccag cgttacgcat
    66841 cagtaattcc agcagggcaa attcttttgg tttcagtgtt aagggttcgc cagccagcgt
    66901 ggcgatacgt ttgccggggt cgagcatgac gttaccgtgg cgcagctcgt tgctggcctg
    66961 gccgttggtt cggcgcatca gagcttccag cctggcggcg acttctatca acgcaaaagg
    67021 tttacacaga taatcgtcag ctcccagacg cagcccttct acacgttccg ccagcgcatc
    67081 gcgcgcggtc aggatcagta ccggctcacg ctgacctttt tctcgccatt cgcgcaaaat
    67141 atcgcgacca tccatgcctg gtaaggttaa atccaggatc accgcatcat aaggtgcgct
    67201 ataaagcgcc tcttttccct gacgaccttg tgtaaaccag tcgacgctaa aacccatttt
    67261 actaaggccc gttttgatgc cgtcgccaat cagcatgtca tcttctatca gtaaaattcg
    67321 cattttttca tccctgcgat aaccgtattt gctgccagta aaccgtgttg taacgcgctc
    67381 tgtacagcac taaattttat ttttttccct taagaagttg ttaaggacta tcttgttaaa
    67441 tgctcggaaa cagacattaa agggagtaat aaacatgaaa aaattcgcag cagtaatcgc
    67501 agtaatggcc ctgtgcagcg caccggtgat ggcagcagag cagggcggtt tttctggccc
    67561 atcggcaacg caaagtcagg ccggaggatt ccaggggccg aacggcagcg taacgactgt
    67621 agaaagcgca aaatccctgc gtgacgacac ctgggtaacc ctgcgcggca atatcgttga
    67681 acgcatctct gacgatctct acgtgttcaa agatgccagc ggtactatca atgttgatat
    67741 cgaccacaaa cgctggaacg gcgtgacggt gacgccgaaa gatacggttg agattcaggg
    67801 tgaagtcgat aaagactgga attctgttga aattgacgtc aaacagatcc gcaaagtaaa
    67861 tccgtaattg ttaccgctcc cgggacgcgt tcccgggaat aatttcgcag ggaggcaaaa
    67921 tgacaaacct gacactggat gtaaacatta tcgatttccc atcaatacct gtggcgatgt
    67981 tgccgcaccg ctgtagccct gaattgctca actacagcgt ggcgaaattt atcatgtggc
    68041 gtaaagagac ggggctttct cctgttaacc aaagccagac ttttggcgtc gcctgggacg
    68101 accctgccac caccgcaccg gaagcgtttc gctttgatat ctgcggcagc gttagcgaac
    68161 cgattcccga taatcgttat ggtgtgagca atggtgaact taccggtgga cgttatgccg
    68221 tggcccgcca cgttggcgag ctggacgata tttcacacac ggtatggggc atcattcgcc
    68281 actggctgcc tgcaagcggc gagaaaatgc gtaaagcacc gattctgttt cactacacca
    68341 atcttgccga aggggtgaca gagcagcgac tggaaacgga tgtttatgtg ccgttggcgt
    68401 gatgcctgac tccagcttcc cttataaatt cctgcttcaa actggcgcta ctaatgcgcc
    68461 actttcttat atggtcaatc actttcctta aagaatacgt tttgtgtggt cactatctcc
    68521 gtacatctaa ctaacctttt aggtgctttt tcattgctgt aattaacctt ttaggttata
    68581 actaaagtaa cagggaggcg ggggttatgg aaaaacgcac accacataca cgtttgagtc
    68641 aggttaaaaa acttgtcaat gccgggcaag ttcgtacaac acgtagtgcc ctgttaaatg
    68701 cagatgagtt aggtttggat tttgatggta tgtgtaatgt tatcattgga ttatcagaga
    68761 gcgactttta taaaagcatg accacctact ctgatcatac tatctggcag gatgtttaca
    68821 gacccaggct tgttacaggc caggtttatc ttaaaattac ggtaattcat gacgtactga
    68881 tcgtctcgtt taaggagaag taatatgaaa tgtccggttt gccaccaggg agaaatggtt
    68941 tctggcatta aagatattcc atacaccttc cgtggacgaa aaacagtatt gaaaggtatc
    69001 cacggtttat attgtgtcca ttgcgaagag agcatcatga ataaagaaga gtcagatgct
    69061 ttcatggcgc aagtaaaggc atttcgggct tcggtgaatg ccgaaacagt ggcacctgaa
    69121 tttatagtga aggttcgaaa aaagctctct cttacccaaa aagaggcaag cgaaattttt
    69181 gggggaggtg taaatgcgtt ttcgcgttac gaaaaaggca atgcccaacc tcatccttcc
    69241 acaatcaaac ttttacgtgt tctggataag catccagaac tattgaatga aatccgttaa
    69301 ttaatggaaa agcgggggca actcgctttt ttctttaccg gagtgtgagc caaatcactc
    69361 gcgataagtt tgagcgaaca atgccacagg cgtattgttg ttgggcttat aatttctccg
    69421 gtaaggaata ccatgtatac gcgaaattta ttatggctgg tcagcctggt aagtgcggct
    69481 cctctctacg ctgctgacgt tcccgccaac acaccgctcg ccccgcaaca agtctttcgt
    69541 tacaacaatc atagcgaccc aggtacgctc gacccgcaaa aggtggagga gaatactgcc
    69601 gcgcagattg tgctggatct gtttgaaggt ctggtatgga tggacggtga aggccaggtg
    69661 cagcccgctc aggctgaacg ctgggagata ctggacggcg gcaagcgcta tattttccat
    69721 ctgcgtagcg gtttgcagtg gtccgacggt cagcctctga cggcagagga ttttgtcctc
    69781 ggctggcagc gcgcggttga cccgaaaacg gcaagccctt ttgctggcta tctggcacag
    69841 gcgcacatta acaatgccgc agctattgtt gcgggtaaag cagatgttac atcgctgggt
    69901 gtcaaagcga cggatgatcg tactcttgaa gttacgcttg agcagccggt tccttggttc
    69961 acgacgatgc tcgcctggcc gacgctgttc ccggttcctc atcatgtcat cgctaaacat
    70021 ggcgatagct ggagtaagcc agagaacatg gtttacaacg gtgcctttgt gcttgatcag
    70081 tgggtagtta acgaaaagat tactgcacgc aaaaatccaa agtaccgcga tgcgcaacat
    70141 acagtattgc aacaggttga gtatctggcg ctagataatt cggtcaccgg ctataaccgc
    70201 tatcgcgcgg gagaggtcga tctcacctgg gttccggcgc agcaaattcc cgccattgaa
    70261 aaatcactgc ctggcgagct acgaattatt ccgcgtctga acagcgaata ttacaacttc
    70321 aaccttgaga aaccgccatt taacgatgtg cgagtgcgtc gggcgctata tcttacggtt
    70381 gatcgacagc ttattgcgca aaaggtactg gggttgagaa cgcccgcaac cacgctgacg
    70441 ccgccagagg taaaaggctt tagcgcgacg acgttcgatg aactgcaaaa gccaatgagt
    70501 gagcgcgtcg cgatggcaaa agccttgctg aaacaggcgg gatacgacgc ctctcatccg
    70561 ctacgctttg agctgttcta caacaagtac gatctgcatg aaaagaccgc gatagcgttg
    70621 tcttccgaat ggaaaaaatg gctgggtgca caggtgacgc tgcgcacaat ggagtggaaa
    70681 acctatcttg atgcccgacg agccggtgat ttcatgctgt ctcggcagtc gtgggatgcg
    70741 acgtacaatg atgcttccag cttcctgaac acgctcaaaa gcgatagtga agaaaacgtc
    70801 ggtcactgga aaaatgcgca gtatgacgcc ttactaaacc aggccacgca gatcactgat
    70861 gcgacaaagc gtaatgcgtt gtatcagcag gcagaagtga tcatcaacca acaggcaccg
    70921 ctgattccta tctactatca gccgttaatc aaactgctta aaccctacgt tggcggtttt
    70981 ccgctgcata atccccagga ttatgtctac agcaaagagt tgtatatcaa ggcacattga
    71041 tgcctttatc gccgtcggag cacgtccggc ggcgctattc acgctgatct cctgtgactc
    71101 gacgcggcag ataatgtagt atctccggca atattgcccc tttgaaggct ggcgaataag
    71161 ttgaggaatc agaattaatg agcgatatgg cagagcgcct tgcgctacat gaatttacgg
    71221 aaaacgccta cttaaactac tccatgtacg tgatcatgga ccgtgcgttg ccgtttattg
    71281 gtgatggtct gaaacctgtt cagcgccgca ttgtgtatgc gatgtctgaa ctgggcctga
    71341 atgccagcgc caaatttaaa aaatcggccc gtaccgtcgg tgacgtactg ggtaaatacc
    71401 atccgcacgg cgatatcgcc tgttatgaag cgatggtcct gatggcgcaa ccgttctctt
    71461 accgttatcc gctggttgat ggtcagggga actggggcgc gccggacgat ccgaaatcgt
    71521 tcgcggcaat gcgttacacc gaatcccggt tgtcgaaata ttccgagctg ctattgagcg
    71581 agctggggca ggggacggct gactgggtgc caaacttcga cggcactttg caggagccga
    71641 aaatgctacc tgcccgtctg ccaaacattt tgcttaacgg caccaccggt attgccgtcg
    71701 gcatggcgac cgatattcca ccgcataacc tgcgtgaagt ggctcaggcg gcaatcgcat
    71761 taatcgacca gccgaaaacc acgctcgatc agctgctgga tatcgtgcag gggccggatt
    71821 atccgactga agcggaaatt atcacttcgc gcgccgagat ccgtaaaatc tacgagaacg
    71881 gacgtggttc agtgcgtatg cgcgcggtgt ggaagaaaga agatggcgcg gtggttatca
    71941 gcgcattgcc gcatcaggtt tcaggtgcgc gcgtactgga gcaaattgct gcgcaaatgc
    72001 gcaacaaaaa gctgccgatg gttgacgatc tgcgcgatga atctgaccac gagaacccga
    72061 cccgcctggt gattgtgccg cgttccaacc gcgtggatat ggatcaggtg atgaaccacc
    72121 tcttcgctac caccgatctg gaaaagagct atcgtattaa ccttaatatg atcggtctgg
    72181 atggtcgtcc ggcggtgaaa aacctgctgg aaatcctctc cgaatggctg gtgttccgcc
    72241 gcgataccgt gcgccgccga ctgaactatc gtctggagaa agtcctcaag cgcctgcata
    72301 tcctcgaagg tttgctggtg gcgtttctca atatcgacga agtgattgag atcattcgta
    72361 atgaagatga accgaaaccg gcgctgatgt cgcggtttgg ccttacggaa acccaggcgg
    72421 aagcgatcct cgaactgaaa ctgcgtcatc ttgccaaact ggaagagatg aagattcgcg
    72481 gtgagcagag tgaactggaa aaagagcgcg accagttgca gggcattttg gcttccgagc
    72541 gtaaaatgaa taacctgctg aagaaagaac tgcaggcaga cgcgcaagcc tacggtgacg
    72601 atcgtcgttc gccgttgcag gaacgcgaag aagcgaaagc gatgagcgag cacgacatgc
    72661 tgccgtctga acctgtcacc attgtgctgt cgcagatggg ctgggtacgc agcgctaaag
    72721 gccatgatat cgacgcgccg ggcctgaatt ataaagcggg tgatagcttc aaagcggcgg
    72781 tgaaaggtaa gagcaaccaa ccggtagtgt ttgttgattc caccggtcgt agctatgcca
    72841 ttgacccgat tacgctgccg tcggcgcgtg gtcagggcga gccgctcacc ggcaaattaa
    72901 cgttgccgcc tggggcgacc gttgaccata tgctgatgga aagcgacgat cagaaactgc
    72961 tgatggcttc cgatgcgggt tacggtttcg tctgcacctt taacgatctg gtggcgcgta
    73021 accgtgcagg taaggctttg atcaccttac cggaaaatgc ccatgttatg ccgccggtgg
    73081 tgattgaaga tgcttccgat atgctgctgg caatcactca ggcaggccgt atgttgatgt
    73141 tcccggtaag tgatctgccg cagctgtcga agggcaaagg caacaagatt atcaacattc
    73201 catcggcaga agccgcgcgt ggagaagatg gtctggcgca attgtacgtt ctgccgccgc
    73261 aaagcacgct gaccattcat gttgggaaac gcaaaattaa actgcgcccg gaagagttac
    73321 agaaagtcac tggcgaacgt ggacgccgcg gtacgttgat gcgcggtttg cagcgtatcg
    73381 atcgtgttga gatcgactct cctcgccgtg ccagcagcgg tgatagcgaa gagtaatatg
    73441 ccggggaggg cgctgtttcc tcccgcttgc aaggaacgcc ggatgaaacg ttaagtcgtc
    73501 atccggcagc gtattcaacc cccggaaacg ataaaaattc ccttgaaacc gttgtttatt
    73561 catgcgttgc gattaacaat acgcttttcc agagagcggc ttttaacaat gccctaacct
    73621 gatttcaggt gacgtacaat gccagtctct cagaccttca gagggtgtta tgctatatat
    73681 ctttcgtctt attattaccg tgatttacag catcttagtc tgtgtattcg gctccattta
    73741 ctgccttttc agcccgcgta acccgaaaca tgtggccacc tttgggcata tgtttggccg
    73801 tcttgcgccg ctgtttggcc tgaaagttga gtgccgtaaa cctacagacg ctgaaagcta
    73861 cggcaatgct atctatatcg ctaaccacca gaacaactat gacatggtga cagcatcgaa
    73921 catcgtgcaa ccgccgacgg tgacggtagg taaaaagagc ttgctgtgga tccccttctt
    73981 cgggcagttg tactggttaa ccggcaactt attgatcgac agaaacaatc gcactaaagc
    74041 tcacggcacc attgcggaag tagtgaatca cttcaaaaaa cgccgtattt ccatctggat
    74101 gttcccggaa ggaacccgca gccgtggtcg cggcctgcta ccgttcaaga ctggagcatt
    74161 tcacgcggca attgcggcgg gcgtcccgat tattcccgtg tgcgtctcta caacttcgaa
    74221 taagattaat cttaatcgac tgcacaacgg tctggtgatt gtcgaaatgc tgccgccaat
    74281 tgacgtcagt cagtatggca aagatcaggt tcgtgagctg gctgcccatt gtcgttcgat
    74341 aatggaacaa aaaatcgccg agctcgataa agaagtcgca gaacgcgaag ccgccggaaa
    74401 agtttaagtc ggcaatctgt atttttgcgg ggaacacttt cctgcacggt attactttag
    74461 ccagttttac atggagcaaa tatgtcactc agtcggcgtc agttcattca ggcatcgggg
    74521 attgcacttt gtgcaggcgc tgttcccctg aaggccagcg cagccgggca acagcaaccg
    74581 ctacccgttc cgccgctact tgaatctcgc cgtgggcaac cgctgtttat gactgtacaa
    74641 cgtgcgcact ggtcatttac gccagggaca cgcgcgtcgg tctggggaat caatggtcgt
    74701 tacctggggc cgactatccg cgtctggaag ggcgacgatg ttaagcttat ttacagcaac
    74761 cgcctgacag aaaatgtctc aatgacggtg gccgggctac aggtaccagg cccgctgatg
    74821 ggcggtccgg cacggatgat gtcgccaaac gctgactggg cacccgtact gcccattcgc
    74881 cagaacgcag ctactctgtg gtatcacgcc aatactccca accgcacggc tcagcaggtc
    74941 tataacggcc ttgccggaat gtggctggtg gaagatgaag tcagcaagtc gctgcctatc
    75001 cccaaccatt atggtgtgga tgattttccg gtcattatcc aggataaacg gctggataac
    75061 tttggtacgc cagaatacaa cgaaccggga agcggcggct ttgttggtga tacgctgctg
    75121 gttaacggtg tacaaagccc gtacgttgaa gtctcgcgtg gctgggtgcg cttgcgactg
    75181 ctgaacgcgt cgaactctcg tcgctatcaa ctacagatga acgatggtcg cccgttacat
    75241 gtgatttctg gcgatcaggg attcctgcct gctcctgtat cggtgaagca actttcgctg
    75301 gcaccgggcg agcgccgcga gattctggtg gatatgagca acggcgatga agtgtcgatc
    75361 acctgtggcg aagcggcgag cattgttgat cgtattcgtg gcttctttga gccatccagt
    75421 attctggttt ctaccctggt gctaacgctg cgcccaaccg gccttctgcc gctggtcaca
    75481 gacagtcttc cgatgcgctt gctgccaact gaaatcatgg ctggttcgcc aattcgcagt
    75541 cgcgatatca gtctgggtga tgacccgggt attaatggac agctgtggga cgtcaaccgt
    75601 attgatgtca ccgcgcagca aggaacgtgg gaacgctgga cggtacgcgc ggacgagccg
    75661 caagcgttcc atattgaagg cgtaatgttc cagatccgta acgtgaatgg cgcgatgccg
    75721 ttcccggaag acagaggctg gaaagatacc gtttgggttg acggacaagt ggagctgctt
    75781 gtttatttcg gtcagccttc ctgggcgcac ttcccgttct acttcaacag tcagacgctg
    75841 gaaatggcgg accgtggctc gattgggcaa ctgttggtca atccggtacc gtaatcttca
    75901 tactcgcccg aggagggcga gtatggaata cctttcgatt tctctttcgt tccggcttat
    75961 aatccccggc ctttgattat ttttttatca tttctggaag caaaatgagc tctatctccc
    76021 tgatccaacc ggatcgcgac ctgttctcct ggccgcagta ctgggccgcc tgttttggac
    76081 cggcaccgtt tttgccgatg tctcgtgaag agatggatca acttggctgg gatagctgcg
    76141 acatcatttt ggttactggc gacgcgtatg tcgatcaccc aagcttcggg atggcgattt
    76201 gcggtcgtat gctggaagcg cagggctttc gcgtcgggat catcgcccag ccggactgga
    76261 gcagcaaaga cgactttatg cgtctgggta aaccgaatct gtttttcggt gttactgctg
    76321 gcaacatgga ttcgatgatc aaccgttata ccgccgatcg ccgtttacgt catgacgatg
    76381 cctacacgcc ggataacgtc gcgggtaagc gcccggatcg cgccacactg gtttataccc
    76441 agcgttgtaa agaggcgtgg aaagatgtac cggtgatcct cggcggtatt gaggctagtc
    76501 tgcgccgtac cgcgcattat gattactggt ccgataccgt gcgccgttcc gtgctggtgg
    76561 attcgaaagc cgacatgctg atgtttggta acggtgagcg tccgctggtg gaagtggcgc
    76621 atcgtctggc gatgggcgag ccaattagtg aaatccgcga tgtgcgtaat accgcgatta
    76681 tcgtgaaaga ggcgctgcct ggctggagcg gcgtggattc cacccgtctt gatacccctg
    76741 gaaaaatcga cccaatcccg catccgtatg gtgaagattt gccgtgcgcg gataacaaac
    76801 cggtggcacc gaaaaagcag gaagccaaag ccgtaaccgt gcagccaccg cgcccgaaac
    76861 cgtgggaaaa aacctacgtg ttgctgcctt ctttcgagaa agtgaagggc gataaagtgc
    76921 tgtacgccca tgcttcgcgt attctgcacc acgaaaccaa cccaggctgt gcccgcgcat
    76981 tgatgcaaaa acacggcgac cgctatgtgt ggatcaaccc gcctgctatt ccgctttcta
    77041 ccgaagagat ggacagcgtt tttgcgctgc catacaagcg cgtgccacat ccggcctatg
    77101 gcaatgcccg tattccggct tacgaaatga tccgtttttc ggtcaacatt atgcgtggct
    77161 gctttggcgg ctgctctttc tgttctatca ccgagcacga agggcgcatt attcagagcc
    77221 gttccgaaga ttcgatcatt aatgagatcg aagcgatccg cgacaccgtt ccaggtttta
    77281 cgggcgtgat ttccgatctt ggtgggccaa ctgccaacat gtatatgttg cgctgcaaat
    77341 cgccacgcgc tgaacaaact tgtcgccgtt tgtcgtgcgt ttatccggat atttgtccgc
    77401 acatggacac gaaccacgaa ccaacgatca acctctatcg ccgtgcgcgt gatctgaaag
    77461 gcattaaaaa gatcctgatt gcctctggtg tgcgttatga catagccgta gaagatccgc
    77521 gctatatcaa agaactggcg acccatcacg tcggcggtta tctgaagatt gccccggaac
    77581 ataccgaaga agggccgtta tcgaagatga tgaagccggg catgggcagc tatgaccgct
    77641 ttaaagagct gttcgatact tactcaaaac aggcaggtaa agagcagtat ctgatcccct
    77701 atttcatctc cgcgcacccc ggtacgcgtg atgaagatat ggtgaatctg gcgctgtggc
    77761 tgaaaaagca ccgcttccgc ctcgaccagg tgcagaactt ctatccgtcg ccgctggcga
    77821 actcaaccac catgtattac accgggaaaa acccgctggc gaagattggt tataagagcg
    77881 aagacgtctt cgtaccgaag ggcgacaaac agcgtcgttt gcataaagcg ttgttgcgtt
    77941 accacgatcc ggcaaactgg ccgttaatcc gccaggcgct ggaagcgatg ggcaaaaagc
    78001 atctgattgg cagccgtcgc gattgcttag tgcctgcgcc aaccattgaa gagatgcgtg
    78061 aagctcgtcg ccagaaccgc aatacccgtc cggcgttgac gaaacatacg ccgatggcga
    78121 cccagcgtca gacgcctgct acggcaaaaa aagcgtcgtc tacgcaatct cgtccggtga
    78181 atgctggtgc gaagaaacgg cctaaagcgg cggttggacg ttaaaaaata tccccggcaa
    78241 ctgacacgct accggggatt tttttatcat tctgcgtaca tacgcaagca gcgcaggcaa
    78301 tcaaaatagt cctgagcatc aagacgcgta ttggctctgt tagcttcgga tttggcagca
    78361 tagatttgtg ggcgtagtgc tttcatatcc tgctcaagcc gggcgatttt ggcatttaat
    78421 gtttgcacat tgctcgccag ctgattgacc ttttgctcat tgacgcagcc actgagcaag
    78481 caggttgcca gaacaacagc tcccacggtg aaaatcgttt tcatatagtg ttccctttta
    78541 tttattgttc cggctaattg ttatttaatt cagctcattg ctttaatctg gcggcagttt
    78601 ttgcctcaat ctgcttaagt aggctgccgc tgttcgggcg gataaaatca gcgatttccc
    78661 acttgccttc gcgccgctgt aaaaccagtt cttgctcttc gttgttgcca ttggtcagcg
    78721 taaaacgaac atgaatgcgg gcggaatcgg cggttttctc caggactcga atttcatcaa
    78781 cgcgtgaaaa tagcgactct tcgccaaaac agatcggatt gtcgtaaata aattgccgtt
    78841 ctatttgcat atcttcatcg ggattattgg ccgtcttgaa ggtggtttta tagaaggcat
    78901 taaccgcctg gcggtaagag tcggaaacca cccacgtcca cggccagcta taatcagcaa
    78961 tatgttcaaa gagttcggca acaaaggctt ccggctgttc tttttgcaac gaagctaaag
    79021 cggccagcgt tttttcattc tcgctattaa ctgcttgtaa gacgctgcca tgattgctga
    79081 caatatcgtc aatgacccaa cgaccatttt ccgccaccat tttcagtgtc tgcgtggtct
    79141 tttctttatc atctttaaag atacgaaagc gcacaacggc atcggcatga tcggcgtcag
    79201 tttgggttat cgcgacgctt tctagcacca gatcgccaaa atcctgacaa tcacaaaccg
    79261 gatcataatc cagccagcca atattgcccg gcagcgtaag attgtcgttc agggtaagcg
    79321 cctgttgaat acgcgcagaa gttatcgccc gctctccggt ttcaccaaaa taagaggcag
    79381 tggcatctga tttatagttc tgataaatat gccggactgt ttgttctacg gtctgtgagg
    79441 ggggctgtgc gtgtacggta aaacttgcca gggctgctaa aaacagaagt attattttca
    79501 tctgtttttc acctctccgt tgcgtttatc ccggatatag atgagttgtt cttttaagag
    79561 cttccggctc tgcatgatga tgtccttata tttggcattc ctgaacaagg aagagtaaca
    79621 acgggcggga cgcgagggga ataaatgatt tctgaaaagt ccggtagcgg aacattaccg
    79681 ccaccgggag aatttgcatg ttagccgccg aactggtcag gatcgggacc gagacgcttg
    79741 ccctgatcga gttttgcaat ttcgccgagt tcgtctttgt cgagacggaa atcccagaca
    79801 tcaaagtttt cggcaatacg tgaaggtgtg accgatttcg ggatcaccac caggccgcta
    79861 tccagatgcc agcggataac aatctgcgcc ggggttttgc cgtatttatc tgccagatcg
    79921 cgaatgactt tctgatcgaa aacgcctttc cctccttgcg ctaatgggct ccaggattcg
    79981 gtctggattt tgtgtgtcgc gttccaggcg tgtagctggc gttgttgcat cagcggatga
    80041 agttcgatct ggtttatcac aggcgtcacg ccagtttcat caatcaggcg ttgcaggtga
    80101 tggatctgga agttgcacac gccgatgctt ttgattaatc cctctttttg caattcgatc
    80161 atgcctttcc atgcttcgac ataatggtcg atagcgggaa cgggccagtg cattaagtag
    80221 aggtcgatat aatcaagctg gagttttttc aggctgtcga gcagggcttc gcgggggcgc
    80281 ttgtggtcgt cgttccacag cttagtggtg atgaacagtt cttctctgtt gactgaggca
    80341 tttttcaggg ctttgccgac accttcttcg ttcttgtagg ccgcggcggt atcaatcgag
    80401 cgataaccca cttctaacgc tttttgaatg gcggtgatta cttcctcatt acttgcttgc
    80461 cagacgccca gtcccagctg gggcatgaca ttgccatcct gtagcttaat aacggttgga
    80521 ttagccatac gttcctcctt tatatgaact caccggagca ggctccggtg aggtgttgtg
    80581 aacttaagtc tggacgaaat gcccgaaaac gaaagtttga ggcgtaaaaa gcttagcggg
    80641 cggcttcgta tatacggcgg ctgacatcca acgtaatgtc atgattttcg cccagttggg
    80701 tcatgccgtg ctcttccagt tttttcagca aagccgggat ggagctgccg tccagaccgt
    80761 agtcggagag gtgggtcggc acgcctaatt gctcaaagaa attgcgggtt gcggcaatcg
    80821 cggcgtcaat acgctcatca tcggaacctt cagtgatgtt ccagacgcgt tcagcatatt
    80881 gcagcagctt agcgcgcttg gtatcgcgtt tttcattcca cagtgcaggc aggacgatag
    80941 ccagtgtttg cgcgtgatcc agaccgtgca tcgcagtcag ttcgtggccc agcatatgcg
    81001 ttgcccagtc ctgcggtacg ccagcgccaa tcaaaccgtt cagcgcctga gtcgccgccc
    81061 acatgacgtt ggcgcgcaca tcgtagtttt ctggctcttt cagggctttc ggaccatctt
    81121 cgattagcgt cagcaaaatg ccttctgcga aacggtcctg aattttggca tcaaccggtt
    81181 tggtaacata ctgttccacg gtgtgtacaa aggcgtccac tacgccgtta gccacctgac
    81241 gcggcggcag ggtgtaggta taaaccggat cgagcacggc aaataccggc tgaacatggg
    81301 cagaatggaa cgcctgcttg tcgcctgtgg ttttacggga gatcaccgcg cctgcgttgg
    81361 attctgaacc ggttgctggc agcgtcagca cacagcccat cgggatggcg cttttaatct
    81421 ctttaccgcc cgtttgcaga atgtgccacg gatcgatatt ttccggatag ttagccgctg
    81481 cggcgataaa tttggtgccg tccagtacag aaccgccgcc aaccgccagc aggaaagtca
    81541 ctttctgttc gcgaaccagt ttcacggcgt tcatcagcgt ttcataagcc gggtttggct
    81601 caataccgcc aaattccagc acgtccatgc ctttcagggc atccagaact tgatcgagaa
    81661 cgccggtttt tttcacgctg ccgccgccgt aggtaatcaa tacgcgagca tcgtgaggaa
    81721 tttgttcgcg taaaccagcg attgcgcctt taccaaacag aatgcgggtt ggggtgtgca
    81781 gattaaagtt gttcattact tgctcccttt gctgggccaa tatgagggca gagaacgatc
    81841 tgcctgatgt ttttcattgt gatcgccagc gccctggctc tcaatgctca tttctgccaa
    81901 tgtcttgcct atttctccag agtgctggag aaatgctaca aaattgcgca caatcaaatt
    81961 gccgcattat tcctaagaaa ttacgcgata tgaaacgtga agagatttgc cgcttgctgg
    82021 cggataaagt taataaactg aaaaataaag aaaatagttt gtcagaactg ttgcccgatg
    82081 tgcgtttgtt gtatggcgag acgcctttcg cacgtacacc ggtgatgtac gagcctggca
    82141 tcataattct cttttccggg cataaaatcg gttatatcaa tgaacgcgtg tttcgttatg
    82201 atgccaatga atacctgctg ctgacggtgc cgttgccgtt tgagtgcgaa acctatgcca
    82261 cgtcagaggt gccgctggca gggttgcgtc tcaatgtcga tattttgcag ttacaggaac
    82321 tgttgatgga cattggcgaa gatgagcatt tccagccgtc gatggcagcc agcgggatta
    82381 actccgccac gttatcagaa gagattttat gcgcggcgga gcggttactc gacgtgatgg
    82441 agcgaccact ggatgcgcgt attctcggca aacagatcat ccgcgaaatt ctgtactacg
    82501 tgctgaccgg accttgcggc ggcgcgttac tggcgctggt cagtcgccag actcacttca
    82561 gtctgattag ccgcgtgctg aaacggattg agaataaata caccgaaaac ctgagcgtcg
    82621 agcaactggc ggcagaagcc aacatgagcg tatcggcgtt ccaccataat tttaagtctg
    82681 tcaccagtac ctcgccgttg cagtatttga agaattaccg tctgcataag gcgcggatga
    82741 tgatcatcca tgacggcatg aaggccagcg cagcagcgat gcgcgtcggc tatgaaagcg
    82801 catcgcaatt tagccgtgag tttaaacgtt acttcggtgt gacgccgggg gaagatgcgg
    82861 caagaatgcg ggcgatgcag gggaattaaa tacgttcccg gtacagcaac cgggaacggg
    82921 gaaaatcgtc aggcgttaca gtattttttt taatcaccac aaacagcgtg cctaacaagc
    82981 cagcggttaa caaggcaatt ggcaggatca tcaggaacgt cattacctga tcttcatggc
    83041 gtttaacgaa cggaatcata cttaaggcat agccaaaact ggttaccacg ctgacccaca
    83101 gcaatccact taaccagttg aaaaactgga agcggcggtt tggcagaccg gaaattcccg
    83161 ccatggttgg cagcagcgta cggacaaatg caagaaaacg tccagccagc agcgccagca
    83221 gaccgtggcg gtcaaacatg caggtggcgc gctggtgata tttagcagga agctgtgcca
    83281 gccagccttt caccgttttg gtattcccta accagcgccc ctgaatataa cttagccagc
    83341 agcccagact tgctgcggcg gtcagaatcg caatcgtagg cagaaaatcc ataaccccct
    83401 gggcaatcaa tgcgcctgcc agtatcaaca agctgtcgcc tggcaaaaat gaggcgggca
    83461 gcaggccgtt ttctaaaaac agcgtggcaa acatgacaaa gtaaacaacg ctaacaatat
    83521 gaggatccgc cagcgcggca aagtcgtgtt gccagagcgc agcgatgata tcttgaataa
    83581 cagccatgga ctttcctgtg gaacagcgtt taagccttta tctgcctatt gtactcctga
    83641 atcgtccggg acgccttgat cccggacgca acaatttaag acttttcaca ataaaatgtc
    83701 tgcaaaattg tccaaaagtg gcaatgttat acaattcgcg caaaaccggc gtccagatcg
    83761 gcaatcagat cgtcgacatc ttccagacca atatgcaggc gaatcaaggt cccgctaaaa
    83821 tcgatctcgc cttgtgggcg aatggcggcg atatgttctg gttgatttgc caggatcaac
    83881 gattcatacc cgccccacga gtaggccatg ctgaataaac tgaagttatc cagatagttc
    83941 gccagctctt cattattgag ttttttctta agcacaaagg aaaatagccc gctgctgcct
    84001 gtaaagtctc gtttccagaa ttcgtgacct ttactgccag gcagagcagg gtggttaact
    84061 cgcgcaactt gcggatgttc tgccagccat tcagccactt tcagactgct ttcatgatgt
    84121 tgacgcaaac gcacacctaa tgtgcgcagg ccacggctgg ttatataggc ggtatcggca
    84181 tcgaccatct ggcccatcag ataggcattt tcccgtagct gctcccagca acgggcattg
    84241 cacacggcag tgccaatcat cgcatctgaa tgcccaacca gatatttggt ggcggcttga
    84301 atagaaacat cgatgccaaa atccagcgcc ttaaacagca caccggctgc ccaggtgttg
    84361 tcgatcataa tgatggcatc cggcaccaca ctgcgtacgg cggcaacaat cgccggaacg
    84421 tcgtggactt ccatggtgat ggagcctggc gattccagaa acacgatttt agtgtttggc
    84481 tgcagatgct taacgatatc ggcaccaatc agcggatcaa accatgatgt cgttacgccc
    84541 agtttgctga ggattttgct acagaaatcc tgactcggtt cataggcggt gttggtcatc
    84601 aacacatgat cgccctgttc gataaaagca agaatggaat tagcaaccgc tgccgccccg
    84661 cagggaaata gcacgcagcc tgcgccacct tccagttcac acatcgcttg ttgtaaggag
    84721 aaatgggtta acgttccgcg ccgtccatag aacaactctc cattggcgcg attacgtgtc
    84781 gcgtgttttt tggcttctac actgtcaaag accagcgaag aagcgcgctg aatcacgcta
    84841 tttaccgcgc cgagagtgta ttttttgctg cgtcctgcat tcaccagttg agtatcaagc
    84901 tttttgtccg ccatgtcggg attcctgttt ttatacgtct ggatgtctaa actagcatga
    84961 atattcgcgg gcgcatcctg aagaattggc ataaagcaga aaattttcgc aaatggcgtg
    85021 ctggtgactt ttttactgcg taaacgcaag gaaaagaaag caaaggtacg gcagtatgca
    85081 aatagtaatg agaacgacta tcaattcgac gtcgttttga tattattatg cgcagatttt
    85141 gtgacttgcg tcctggagat acacagtggg taataattta atgcagacgg acctttccgt
    85201 ctggggtatg tatcagcacg ccgatattgt cgttaagtgc gtgatgattg ggcttatttt
    85261 ggcctccgta gtcacctggg caatcttctt cagtaagagc gtagagttct tcaatcagaa
    85321 gcgtcgcctt aagcgcgagc agcaactgct ggctgaagcg cgttccttaa accaggccaa
    85381 cgatatcgcc gctgattttg gtagcaaaag cttaagcctg catttgctca atgaagcgca
    85441 gaatgagctg gaactgtcag aaggcagcga cgataacgaa ggtattaaag aacgtaccag
    85501 cttccgtctg gagcgtcggg tcgccgcagt gggtcgtcaa atgggccgcg gtaacggcta
    85561 tctggcaacc atcggcgcga tttctccgtt cgttggtctg tttggtacgg tctggggcat
    85621 catgaacagc tttattggta tcgcgcaaac gcagaccact aacctggcag tcgttgcgcc
    85681 gggtatcgca gaagctctgt tagcaacggc aatcggcctc gtggcagcga ttcctgcggt
    85741 cgttatctat aacgtatttg cacgccagat tggcggcttt aaagcgatgc tgggtgatgt
    85801 tgcagcgcag gtattgttgc tgcaaagccg tgacctggat ctggaagcca gcgccgctgc
    85861 gcatccggtt cgtgtcgcac aaaaattacg cgcaggataa tatccgatgg caatgcatct
    85921 taacgaaaac ctcgacgata acggcgaaat gcatgatatc aacgtgacgc cgtttatcga
    85981 cgtgatgttg gttctgctga ttatctttat ggtggcggca ccgttagcga cggtagatgt
    86041 gaaggtgaac ttgcctgctt ctaccagcac gccgcagccg cgcccggaaa aaccggttta
    86101 tctgtcggtg aaggcagaca actcgatgtt tatcggtaac gatccggtca ccgatgaaac
    86161 aatgattacg gcgttgaatg cgttaaccga aggcaagaaa gacaccacca tcttcttccg
    86221 agcggacaaa accgtcgatt acgagacgtt gatgaaggta atggatacgc tgcatcaggc
    86281 gggttacctg aagataggtc tggtcggcga agaaaccgcc aaagcgaagt aaagtagaat
    86341 tgcctgatgc gctacgctca tcaggcctac aaaatctatt gcaacatgtt gaatcttcgt
    86401 gcgtttgtag gccggataag gcgttcacga cgcatccggc attaggtgct caatgcctga
    86461 tgcgctacgc ttatcaggcc tacaaaatct attgcaacat gttgaatctt catgcgtttg
    86521 taggcggata aggcgttttc gccacatcag gtaagagtga attcacaatg atgcccggtt
    86581 gcttttcaca accgggcatt tttttaacct aaatgctcgc cgccgcacac gccgtgcact
    86641 tctgcggtga cgtagctcga ctcctgactt gccagataaa catatacagg ggccagttcc
    86701 gccggttgcc ccgcacgttt catcggcgtt tgctgaccaa actgcgggat cttatcctgc
    86761 gtttgtccgc cggaaatttg cagtgctgtc cagatcgggc ctggcgcgac aatattcacc
    86821 cgaatacctt tctccgcgac ctgttttgcc aagccacggc tgtagttcag aatcgccgcc
    86881 ttcgtagccg catagtccag taaatgcgga cttggctggt atgcctggat tgacgaagtg
    86941 gtgatgatac ttgcaccttt cggtagcagg gggatcgctt cctgggttag ccagaacagc
    87001 gcgaaaacgt taatggcaaa ggtcttttga aactgttcgc tggtgaggtc tgcaatatcc
    87061 ggaatggcaa cctgtttccc ggcgaccagc gccataatat ccagcccgcc taacgccttg
    87121 tgcgctttgt gaaccagcga acgggcaaat ttctcatcgc ttaaatcgcc tggcagcaga
    87181 acggctttgc gtccgcattc ttcaatgatc tttttcacat cctgagcgtc ttcttcttcc
    87241 acgggaagat aactgatcgc cacgtcagcc ccttcacgcg cgtaagcgat ggcggcagcg
    87301 cgacctattc cggaatcgcc ccctgtcacc agtgctttac gatctttcag gcgaccgcta
    87361 ccaacatagg ttttctcgcc gcaatccggt accggtgtca tcttcgcctg gatgcctggc
    87421 gtcggttgtt tctgtttggg atattcacca gtgtaatact gcgtggtcgg gtcttttaaa
    87481 tgagacatcg tttttctccc ttcaggttca acgtccttta agggtagacg ttctcgatgc
    87541 gttgataagg gaaccaggaa gatccctaaa cctcagaatt atgcgacaaa ggtttaacgt
    87601 atatgttgat ttgctgttgc gtgctgttta ctcaattgcg atatactgtt gcccgtttta
    87661 actacacgac aggaatgtat ggaacgtttt cttgaaaatg caatgtatgc ttctcgctgg
    87721 ctgcttgccc ccgtgtactt tggcctttcg ctggcgttag ttgccctggc gctgaagttc
    87781 ttccaggaga ttattcacgt actgccgaat atcttctcga tggcggaatc agatttgatc
    87841 ctcgtgttgc tgtcgctggt ggatatgacg ctggttggcg gtttgctggt gatggtgatg
    87901 ttttccggtt atgagaattt cgtctcacag ctggatatct ccgagaacaa agagaagctg
    87961 aactggctgg ggaaaatgga cgcaacgtcg ctgaaaaaca aagtagcagc gtcgattgtg
    88021 gcaatttctt ccattcactt actgcgcgtc tttatggatg cgaaaaatgt cccggataac
    88081 aaactgatgt ggtacgtcat tatccatctg acgtttgtgc tttctgcatt tgtgatgggc
    88141 tatcttgacc gactgactcg tcataatcac tgatctgatt cgggcgcggt tcgcgcgccc
    88201 gttattaaca ggtcatttat cggaagacgc ctgccacaga ttcagctcgc catcggcgat
    88261 atgctgatca atctgcgcca gctccttggt gctaaatgtc agattattca gcgcctgcac
    88321 gttctcctct agttgctccg cgcggctggc accaatcaat accgacgtca cgcgatcatc
    88381 tttcagcaac cagcttaacg ccatttgcgc cattgattgt ccacgctgct gtgccatttc
    88441 attcaataag cgcaggctgt tgaggttggc ttcggtaagc attttcggtg tcagaccacg
    88501 aactttattc ccttcacgat gcatccgtga atcttgcgga atgccgttga gatattttcc
    88561 ggtcagcaat ccctgagcca gaggagtaaa ggcaatacag cccacgccgt tattttgcag
    88621 ggtatccagc aggccgcttt tatccaccca gcggttcagt aaattgtacg aaggttgatg
    88681 aattaacagc ggaattttcc actcgcgcag caactcgacc attttttgcg tccgctctgg
    88741 cgagtaagag gagatcccga catacagcgc cttaccgctt tgtaccgcat gagccagcgc
    88801 agaggcggtt tcttccatcg gcgtattttc atcgacgcga tgagagtaaa agatatcgac
    88861 atactcaagc cccatacgct tcaggctttg gtcgaggctg gcgagcaggt atttacgtga
    88921 accgccagag ccgtaagggc cgggccacat atcgtagcca gccttggtag agataatcag
    88981 ttcatcgcga taagcggcaa aatcctcccg cagcaggcga ccaaagttct cttctgcgct
    89041 tcctggaggc ggcccgtaat tgttggctaa atcaaagtgc gtaatgccca aatcaaacgc
    89101 tttacgcagg atcgcacgct gtgattccag cgcgttaacg tgaccgaaat tgtgccataa
    89161 accgagcgat aacgcgggca ggcgtaaacc actttttccg caatagcggt attgcatctg
    89221 cccgtaacgt tcgggattcg ctaaccagac catgacctct cctttccacc gttcaatttc
    89281 gaaacaatgt ttctagttta gcgattcgcc agcgcgtatc ccgtagtctg gctcacagag
    89341 tgacgaaaaa ctgggcaaaa acacgtgctt atgctttgct taaaaaaaca ccaactgagg
    89401 agtgcaacga tgccgcgttt aaccgccaaa gatttcccac aagagttgct ggattactat
    89461 gactattacg ctcacgggaa aatctcgaaa cgtgagttcc tcaaccttgc ggcgaagtgc
    89521 agtaggcggg atgacggcat tagcgttgtt tgatttgctc aagccaaatt atgcgctggc
    89581 gactcaggta gagtttaccg acccggaaat tgttgctgag tacatcacgt atccttcgcc
    89641 aaatggtcac ggcgaggtgc ggggttatct ggtgaagccc gcaaagatga gcggcaaaac
    89701 gccagccgta gtggtggtgc atgagaatcg tggactgaat ccgtatatcg aagatgtggc
    89761 acggcgagtg gcgaaggcgg ggtatatcgc cctggcacct gacggcttaa gttccgttgg
    89821 aggttatccg ggaaatgatg ataaaggtcg tgagctgcaa cagcaggttg atccaacaaa
    89881 ctgatgaatg atttctttgc cgcaattgag tttatgcaac gctatccgca agcgacaggc
    89941 aaagtgggta ttaccggatt ttgctatggc ggtggcgtat cgaacgcggc ggctgtcgcg
    90001 tatccggaac tggcctgcgc ggtgccgttt tatggtcgtc aggcacccac tgccgatgtg
    90061 gcgaagattg aagcgccttt actactccac ttcgcggaac tggacacccg aatcaacgag
    90121 ggctggcctg cttacgaggc ggcgttgaaa gccaataata aggtttatga ggcgtatatc
    90181 tatccggggg ttaatcacgg attccataat gattccacgc cccgttatga caaatctgcc
    90241 gccgatcttg cctggcaaag gacactgaaa tggttcgata aatatctctc ctgataggtt
    90301 tatctcttac gggattacgt cttaaacaag catgaaaaaa tagcgtgcgc aaaagtcgtt
    90361 ctttgcctaa aatatcgcta tatatgacaa tatatagcga atgaggtgaa cgatgaataa
    90421 ccattttggt aaaggcttaa tggcgggatt aaaagcaacg catgccgaca gtgcggttaa
    90481 tgtgacaaaa ttctgtgccg attataaacg cggttttgta ttaggctact cacaccggat
    90541 gtacgaaaag accggagatc gccagcttag cgcctgggaa gcgggtattc tgacgcgccg
    90601 ctatggactg gataaagaga tggtaatgga tttctttcgt gagaataatt cctgttctac
    90661 gttgcgcttt tttatggccg gttatcgcct cgaaaattga tcaaacatac gtattatctt
    90721 gctttaatta attacactaa tgcttcttcc cttcgtttta gcgccccgcc gcagtatcat
    90781 gatatcgata accataataa atgtgtggta aatggcgcat cgatcgcatt attgattttg
    90841 cgattgaggc aaaatatatg ccaggtcttc gcaacggaat aactataaat gactggagat
    90901 aacaccctca tccattctca cggcattaac cgtcgtgatt tcatgaagct ttgtgcagca
    90961 ttagccgcca ccatggggtt aagtagcaaa gccgctgcag agatggccga atcggttact
    91021 aacccgcagc gtccgccagt tatctggatt ggcgcgcagg agtgcaccgg ttgtacggaa
    91081 tctctgcttc gtgcaacgca tccaacggta gaaaacctcg tactggagac tatctctctg
    91141 gagtatcacg aagtgctttc cgccgccttc ggtcatcagg tcgaagagaa caaacataac
    91201 gctctcgaga agtacaaagg gcagtatgtg ttagtggtgg atggttccat cccattaaaa
    91261 gataacggta tttattgcat ggttgccggt gagccgattg tggatcacat ccgcaaagcg
    91321 gcggaaggcg cagcagccat tatcgctatc ggttcctgct ctgcgtgggg cggtgttgcc
    91381 gcagctggag ttaacccaac tggcgcagtc agcctgcaag aagttctgcc aggcaaaacc
    91441 gttatcaata ttccgggctg cccgccgaac ccgcacaact tcctcgcgac cgttgcgcac
    91501 atcatcactt acggcaaacc gccgaaactg gatgacaaaa accgtccgac cttcgcctat
    91561 ggccgtctga ttcacgaaca ctgcgaacgt cgcccgcact tcgatgctgg tcgttttgcc
    91621 aaagagttcg gtgatgaagg ccaccgcgaa ggctggtgcc tgtaccacct cggctgtaaa
    91681 gggccagaaa cttacggcaa ctgctcaacg ctgcaattct gcgatgttgg cggtgtgtgg
    91741 ccggtggcga ttggtcaccc gtgctatggc tgtaacgaag aaggtatcgg cttccataaa
    91801 ggcatccatc agcttgccaa cgtcgaaaat caaactccgc gttcacagaa accggatgtt
    91861 aacgctaaag agggcggcaa cgtctctgca ggcgctattg gtttgctcgg cggtgtggtt
    91921 gggttggttg ccggtgtcag cgtgatggcg gtgcgtgaac tgggtcgtca gcaaaagaaa
    91981 gataacgctg actcacgggg agaataaccg tgaacagacg taattttatt aaagcagcct
    92041 cctgcggggc attgctgacg ggcgcgctgc cgtctgtcag tcatgcggct gctgaaaacc
    92101 gcccgccaat tccgggatcg ctggggatgt tgtacgactc gaccttgtgc gtaggctgcc
    92161 aggcttgcgt caccaagtgt caggatatca atttccctga acgtaacccg caaggggaac
    92221 agacctggtc gaacaacgac aaactgtcgc cgtataccaa taacatcatt caggtgtgga
    92281 ccagcggcac aggggtcaac aaagaccagg aggagaacgg ctacgcgtac attaagaaac
    92341 agtgtatgca ctgcgtcgat ccgaactgtg tctctgtgtg cccggtctct gcactgaaaa
    92401 aagatccgaa aaccggcatt gtccattacg acaaagatgt gtgcaccggc tgccgttact
    92461 gcatggtcgc ctgtccgtac aacgtgccga agtacgacta caacaacccg tttggtgcgc
    92521 tgcataagtg cgagctgtgc aaccagaaag gtgtggaacg tctcgataaa ggcggtctac
    92581 ctggctgcgt agaagtgtgc ccggcgggcg cggtgatttt cggtacgcgt gaagagctga
    92641 tggcggaggc gaaaaaacgt ctggcgctga agcctggcag cgaataccac tatccgcgtc
    92701 agacgctgaa atctggcgac acttacctgc atacggtgcc gaaatattat ccgcatctgt
    92761 acggcgagaa agagggcggc ggtactcagg ttctggtact gacgggtgtg ccttatgaaa
    92821 atctcgacct gccgaaactg gacgatcttt ctaccggtgc gcgttccgaa aatattcaac
    92881 acaccctgta taaaggcatg atgctaccac tggctgtgct ggcgggctta accgtgctgg
    92941 ttcgtcgcaa caccaaaaac gaccatcacg acggaggaga cgatcatgag tcatgatcca
    93001 caaccgctgg gcggcaaaat catcagtaaa ccggtcatga tttttggacc gttaatcgtc
    93061 atctgtatgc tcctgattgt gaagcgtctg gtgttcggtc tgggctctgt ctctgacctg
    93121 aacggcggct tcccgtgggg cgtgtggatc gcgtttgacc tgctgattgg caccggcttt
    93181 gcctgtggcg gctgggcgct ggcgtgggcg gtatacgtct ttaaccgtgg gcaataccat
    93241 ccgctggtgc gtccggcgct gttggcgagt ctgtttggtt actcactggg tggcttgtcg
    93301 atcactatcg acgtgggtcg ctactggaac ctgccgtact tctacattcc gggtcacttc
    93361 aacgtgaact cggtactgtt cgagacggcg gtctgtatga ccatctatat cggcgtgatg
    93421 gcactggagt ttgctccggc actgtttgaa cgtctggggt ggaaggtgtc gctacagcga
    93481 ctaaataagg tgatgttctt catcatcgcg ctcggtgcgc tgctgccgac catgcaccag
    93541 tcttcaatgg ggtcgctgat gatctcggcg ggctacaagg tgcatccgtt gtggcagagc
    93601 tatgaaatgt tgccgctgtt ctcgctgctg acggcgttca tcatgggctt ctcgattgtc
    93661 atctttgaag gttcgctggt gcaggcgggt ctgcgtggca acggtccgga tgaaaagagt
    93721 ctgtttgtta agctgaccaa caccatcagt gtgttgctgg cgattttcat cgtgctgcgc
    93781 tttggcgagc ttatctatcg cgacaagctg tcgttagcgt ttgccggtga cttctactcc
    93841 gtgatgttct ggattgaagt cctgctgatg ctcttcccgc tggtcgttct gcgtgtggcg
    93901 aagctgcgta atgattcccg catgctgttc ctgtcagcac tgagcgcact gttaggttgt
    93961 gcaacctggc gtctgaccta ttcgctggtg gcattcaacc cgggcggcgg ttacgcctac
    94021 ttcccgacct gggaagaact gttgatttct attggttttg tggctattga gatttgcgct
    94081 tacatcgtac tcattcgtct actgccgata cttcctcctt taaaacaaaa cgatcataat
    94141 cgtcatgagg cgagcaaagc atgagccaga gaattactat tgatccggta acccgtattg
    94201 aggggcattt acgcatcgat tgcgaaatcg aaaatggcgt cgtttcgaaa gcatgggctt
    94261 ccggtaccat gtggcgcggc atggaagaga tcgtgaaaaa ccgcgatccg cgcgatgcat
    94321 ggatgattgt gcaacgtatc tgtggcgtat gtactaccac tcacgcgctg tcttccgttc
    94381 gtgcggcaga aagtgcgctg aatatcgacg ttccggttaa cgcgcaatac atccgtaaca
    94441 tcattctggc tgcgcacacc acgcatgacc atattgttca tttctatcag ctttcggcgc
    94501 tggactgggt ggacatcact tctgcactgc aagctgaccc aaccaaagcc tccgaaatgc
    94561 tgaaaggcgt ttcgacctgg cacctgaaca gtccggaaga gttcaccaaa gttcagaaca
    94621 agatcaaaga tctggttgcc agcggtcagt tgggtatttt cgctaatggc tactggggtc
    94681 atccggcgat gaaactgccg ccggaagtga acctgattgc ggtagcgcac tacctgcaag
    94741 cgttggagtg ccagcgtgac gctaaccgcg tcgtggcgct gctgggcggt aaaacgccgc
    94801 acattcagaa cctggcggta ggtggtgtcg cgaacccgat caacctcgac ggtttgggcg
    94861 tgctgaacct tgagcgcctg atgtacatca agtctttcat cgacaaactg agcgactttg
    94921 ttgagcaggt ttataaggtt gataccgcag ttattgccgc gttctacccg gaatggctga
    94981 cacgcggtaa aggtgcggtg aactacctga gcgtgccgga attcccgacc gacagtaaaa
    95041 acggcagctt cctgttcccg ggcggctaca ttgagaatgc ggatctgtcc tcgtatcgtc
    95101 cgatcacttc tcattccgat gaatacctga tcaaaggcat tcaggaaagc gcgaagcact
    95161 cctggtataa agacgaagcg ccgcaggcac cgtgggaagg caccaccatt ccggcttatg
    95221 atggttggtc tgacgacggg aaatattcct gggtgaaatc accgactttc tacggcaaaa
    95281 cggtagaagt ggggccactg gctaatatgc tggtgaaact ggcggcaggt cgcgaatcta
    95341 cccagaacaa actgaatgaa atcgttgcga tttatcagaa actgactggc aacacgctgg
    95401 aagtggcaca gctgcactcc acgctgggcc gtattattgg tcgtaccgtt cactgctgtg
    95461 aattgcagga tatcctgcaa aaccaataca gtgcactgat caccaatatc ggcaaaggcg
    95521 atcacaccac ctttgtgaag ccgaacattc cggcaacggg tgaattcaaa ggtgttggct
    95581 tcctcgaagc gccgcgcggt atgctctctc actggatggt tattaaagac ggtatcatca
    95641 gcaactacca ggcggttgtt ccatcaacct ggaactctgg tccgcgtaac ttcaatgatg
    95701 acgtcggtcc ttacgagcag tcgctggtgg gtacaccggt tgccgatccg aataaaccgc
    95761 tggaagtggt gcgtaccatt cactcctttg acccgtgcat ggcctgtgcg gtacacgtag
    95821 tggatgctga cggcaacgaa gtggtttcag tgaaggttct gtaatgcgta ttttagtctt
    95881 aggggtcggc aatattttgc tgaccgatga agccatcggt gtgcggattg tcgaagcgtt
    95941 agagcaacga tacattctgc cggattatgt tgagatcctc gatggcggca cggcgggaat
    96001 ggagctgctt ggcgacatgg caaatcgcga tcatttgatt attgcggatg ccattgtctc
    96061 gaaaaagaac gcgccgggaa cgatgatgat cctgcgggat gaagaagttc cggcgttgtt
    96121 taccaacaaa atctctccgc atcagcttgg cctggccgac gtcttgtcgg ccctgcgctt
    96181 caccggcgag tttccgaaaa agctgaccct ggtcggcgtg atcccggaat cgctggagcc
    96241 acacatcggc ttaacgccga cggttgaagc aatgattgaa cctgcgcttg agcaggttct
    96301 ggctgcgctg cgtgaatctg gcgtggaagc catcccacgg gaggcgattc atgactgaag
    96361 agatagcagg tttccagacc tccccgaagg cgcaagtaca ggcagcgttt gaagaaattg
    96421 cccggcgttc gatgcacgat ctctcttttc tgcatccttc aatgccggtg tatgtttctg
    96481 attttacgct gttcgaaggt cagtggacgg ggtgtgtgat caccccgtgg atgctgagtg
    96541 cagttatctt ccccggcccg gatcaactct ggccgctgcg caaagtgagt gaaaaaattg
    96601 gtctgcaact gccgtatggc actatgacct ttaccgttgg cgaactggac ggtgtttcgc
    96661 aatatctctc ctgttcgctg atgtcgccgc tttcgcacag catgtcgatt gaagagggcc
    96721 aacgcctgac ggatgactgc gcacgaatga tcctttcgct gccagtcacg aatccggatg
    96781 taccacacgc agggcgtcgc gccctgctgt ttggtcgcag gagtggcgaa aatgcatgag
    96841 ttgtctcttt gccagagcgc cgttgaaatt atccaacggc aggcggagca gcacgatgtt
    96901 aagcgcgtca ccgccgtgtg gctggaaatt ggcgcgctct cctgcgttga ggagagcgcc
    96961 gtccgtttta gttttgaaat tgtctgccac ggaacggtgg cgcaagggtg cgatttacat
    97021 atcgtctata aacccgccca ggcttggtgc tgggattgca gccaggtggt ggagattcat
    97081 cagcacgatg cgcagtgtcc gctctgtcac ggcgagcggt tgcgtgtcga taccggcgat
    97141 tcgctgatcg tcaaaagtat tgaagttgaa taaccggagt taataatgtg tattggcgtt
    97201 ccaggccagg tgctggctgt cggtgaagat attcaccagc ttgcgcaggt tgaagtatgt
    97261 ggtatcaagc gcgatgtgaa tatcgccctg atttgtgaag gtaaccctgc cgatctactg
    97321 ggccagtggg tgctggtaca cgtcggattt gccatgagca tcatcgacga agatgaagcc
    97381 aaagccacat tagacgcact gcgccaaatg gattacgaca ttaccagcgc gtgatgatta
    97441 gctttcgtag gcctgataag acgcggcagc gtcgcatcag gcattgtgca cgattgccgg
    97501 atgcggcgtg aacgccttat ccggcctgga tggcttgctg cgacgaacac caacccttac
    97561 ccctgacgct tatcttccgt attcgtctcg aaatcactgg cgtcatggcg ctcatgcaac
    97621 tgctcattca gcggtccgtt ggtgcggtta acaatacgcc cacgtttcac cgccggacgt
    97681 tcgcctactt ctttcgccca gcgttgtaca tgcttataac tgcccgcatc aagaaactca
    97741 gcggcatcat acacaccacc taacaccacg ttgccaaacc acggccaaat cgccatatcc
    97801 gcaatggtgt actcatcgcc cgcaacaaac ttatgctgcg ccagttgctt atccagcacg
    97861 tcgagcagac gtttggcttc catggtaaag cggttgatgg cgtactcaat ctttaccggt
    97921 gcgtaatggt aaaagtgacc aaaaccaccg ccgaggaacg gtgccgcgcc ctgtaaccag
    97981 aacagccagt tcatcgtttc agtacgcttt gccaaatcct gcggcaggaa gtagccaaat
    98041 ttctccgcca gataaagcag gatcgaacca gattcaaaca cgcggatcgg cggattatgc
    98101 gtatgatcgc gcagcgccgg gatcttcgag tttgggttca cttcgacaaa gccgctggag
    98161 aattgatcgc catcgccaat acgaatcagc caggcgtcgt actctgcacc agtaacgccc
    98221 agcgccagca gctcctcaag cataatcgtt actttctgac cgttcggcgt tcccagcgaa
    98281 taaagttgca atgggtgttt gccaacgggc agcgtttttt catgcgtcgg accagaaacc
    98341 gggcgattga tattggcgaa cgcgccgcca gcggatttat cccacgtcca gactttcgcg
    98401 ggctgataag tattgtctgt catagtgagt tgccttctga gtggtagtgt tgaagcagtg
    98461 tagcaggtca cttgctgtac agaatgtgaa ttttttaacc ctttgcgcca gccgctgcga
    98521 tgaggtgtat gattagtctg acctctgtcc ggttaatgtc gctgagcagg taatcagagc
    98581 acaacaatta cgccaactgc ttaggttgta ttgttaaagg taaagtgatg agcaaaggaa
    98641 cgaccagcca ggatgccccg ttcgggacat tattgggcta cgccccaggt ggggtagcaa
    98701 tctactcttc agattacagt tctctcgatc cgcaggaata cgaagatgac gccgtattcc
    98761 gtagctatat cgacgacgaa tatatgggcc acaagtggca atgcgttgaa tttgctcgcc
    98821 gttttctctt tctgaattac ggtgtggtct ttactgacgt gggtatggcg tgggagattt
    98881 tctcgctgcg cttcctgcga gaagtggtta atgacaacat cctgccattg caggcatttc
    98941 ctaacggctc gccgcgtgcg ccggtcgcgg gtgcgcttct tatctgggat aaaggcggtg
    99001 aatttaaaga cactggccat gtcgccatca ttacccaatt gcatggcaac aaagtccgta
    99061 ttgcggaaca gaacgtgatt cattccccgt tgccgcaagg gcaacagtgg acgcgcgagc
    99121 tggagatggt ggtcgaaaac ggctgctata ccctgaaaga cacttttgat gacaccacca
    99181 ttctgggctg gatgatccag acggaagata ctgaatacag cttaccgcag ccggaaattg
    99241 caggcgagct gctgaaaatc agcggagcgc gcctggaaaa caaaggccag tttgacggta
    99301 aatggctgga tgaaaaagat ccgctgcaaa acgcctatgt gcaggccaac ggtcaggtga
    99361 tcaatcagga tccttatcat tactacacca ttaccgagag tgccgagcag gagctaatta
    99421 aagccaccaa cgagctgcac ctgatgtatc ttcacgcaac cgacaaggtg ctgaaagatg
    99481 acaacctgct ggcgctgttc gacatcccga aaatcctctg gccacgtttg cgtctctcct
    99541 ggcagcgtcg ccgtcaccat atgatcactg gtcgtatgga tttctgcatg gatgagcgtg
    99601 gcctgaaggt ttacgagtac aacgccgact ccgcctcctg tcataccgaa gcgggcttga
    99661 tcctcgaacg ttgggcggag cagggctata aaggcaacgg cttcaatccg gcggaagggc
    99721 tgattaacga attggctggt gcctggaaac acagtcgtgc acgtccgttt gtccatatca
    99781 tgcaggacaa agatatcgag gaaaactatc acgcgcagtt tatggagcag gcgctgcacc
    99841 aggcgggctt tgaaacgcgt atcttgcgtg gattggatga actgggctgg gatgctgccg
    99901 ggcaactgat tgatggggaa gggcgactgg ttaactgcgt gtggaaaacc tgggcgtggg
    99961 aaaccgcgtt tgatcagatt cgtgaagtta gcgaccgtga gtttgctgcg gtgccaatcc
   100021 gtaccggtca tccgcaaaac gaagtgcgtc ttatcgacgt attgctgcgc ccggaagtgc
   100081 tggtctttga gccgctgtgg acggtgatcc ccggcaacaa agcgattctg ccgatcctct
   100141 ggtcgctgtt cccgcaccat cgttacctgc tggataccga tttcactgtt aatgatgaac
   100201 tggtgaaaac aggttacgca gtgaaaccga tcgccggtcg ctgtggcagc aatatcgacc
   100261 tcgtcagcca tcatgaagag gtgctggaca aaaccagcgg taaatttgcc gagcagaaaa
   100321 acatctatca gcaactgtgg tgtttgccga aagtggacgg taaatacatt caggtatgta
   100381 ccttcaccgt tggcggcaac tacggtggga cgtgtttgcg cggtgatgaa tcactggtca
   100441 tcaaaaaaga gagtgatatt gaaccgttaa ttgtggtgaa aaagtaatac atgttgtagc
   100501 aaccccgtac tgattatgaa gaataatcag tacggggatt aatcaaaata ttcaatattt
   100561 atatattcca atatttattc atttcatatg tgaatatatt aaccagtgga atacctgtgt
   100621 tttgattatt tctaaaggtt ttgaatgaat gcttattgtc tgatacacaa gaaataacac
   100681 tctttttatc gttaaaaaat gatatttcac tttgcccatg ccgttaaagt cactgataat
   100741 gcgtccgttc gtaaattcaa aatggcgtaa tctaatatat gctaaattta tttgttggcc
   100801 ttgatatata cacagggctt ttgttattgc ttgctctggc atttgtgttg ttctacgaag
   100861 caatcaatgg ttttcatgac acggcgaatg cggtggcagc cgttatttat actcgtgcca
   100921 tgcaaccaca acttgctgtg gtgatggcgg cattttttaa cttttttggc gtgttattgg
   100981 gcggacttag cgttgcctat gccattgtcc atatgttgcc aaccgatttg ttgctgaata
   101041 tggggtcaac ccacggcctg gcgatggtct tttccatgct gctggcggcg attatctgga
   101101 acctgggaac gtggttcttc ggtttaccgg cctccagttc gcacaccttg attggtgcga
   101161 ttatcggcat cggtttaacc aacgcgctgt taaccggctc atcggtgatg gatgcgttaa
   101221 acctgcgtga agtgaccaaa attttctcct cgctgattgt ttcccctatc gtcggcctgg
   101281 tcattgcggg aggcctgata ttcctgctgc gacgctactg gagcgggacg aaaaagcgtg
   101341 accgtattca ccgcattccg gaagatcgca aaaagaaaaa aggcaaacgt aaaccgccat
   101401 tctggacgcg tattgcgctg attgtttccg ctgcgggcgt ggcgttttcg cacggcgcga
   101461 acgacggaca aaaagggatc ggcctggtaa tgctggtact ggtggggatt gcccctgctg
   101521 gcttcgtcgt caatatgaat gcgtccggct atgaaattac ccgtacccgc gatgccgtta
   101581 ccaacttcga acactacctg caacagcatc ctgaactgcc gcagaagttg attgcgatgg
   101641 aacctccatt gcctgcagca tcgactgatg gcacgcaagt aacagagttt cactgtcatc
   101701 cggcaaatac ctttgatgct attgcgcgcg ttaaaacgat gctgccaggc aatatggaaa
   101761 gttacgagcc gttaagcgtg agtcagcgca gccagctgcg ccgcattatg ctgtgcatct
   101821 ctgatacctc cgcgaagcta gcgaaactgc caggcgtcag taaagaagac cagaacctgc
   101881 tgaaaaaact tcgcagcgat atgttaagca ccattgagta cgctccggtg tggatcatca
   101941 tggcggtagc actggcgctc ggcattggca ccatgattgg ctggcgtcgt gtagcgatga
   102001 ccatcggtga gaagattggt aagcgcggca tgacgtatgc gcaaggcatg gcggcacaaa
   102061 tgacggcggc agtgtctatc ggtcttgcca gttatattgg gatgcccgtc tccacaacac
   102121 acgtcctctc gtctgcagtt gcagggacga tggtggtgga cggcggtggg ttacagcgta
   102181 aaacggtaac cagcatcctg atggcgtggg tatttacttt accggcggca atttttcttt
   102241 ctggtgggct gtactggata gcattgcagt tgatttaacc ttcctgaaaa tgcccggtcc
   102301 tggcgatcgg gcatttccat ttttgactag tgataaccac gcgcggtcat aaaatccgta
   102361 atcgcttttt ctgcatcaac caacacctgt tccagtggct gattggcatc gatatcaacc
   102421 agttgtgcac caccaaaggt taactgtggc gttatggcaa tcttcctcgc cagcgattcc
   102481 cgtttatggt cgggtttacg tgcacaggca acttcaaggt caacattgag tttgatgacc
   102541 agatcaggct tatggctcgc catccagtga aacgctttac gttcctggct tgccagccat
   102601 gagacaaaac gaccaccttc aacgttaggt gggaacaccg taccatcgta agcgccagga
   102661 atttggtcct gaggataacg gtcggttaga acaattaacc cgcgacgacg acaggcaagc
   102721 atatgacgaa agcgcagtaa gcgacgggcg acaaacgctg taattaccag cgccggaact
   102781 ggtccaggca atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat
   102841 tttcccatca acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa
   102901 tgaactcttt cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt
   102961 gacttgcctg aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg
   103021 aatatatcct ttaatttaaa tatccattaa aaatatttat ttggttaata tgtttttatg
   103081 aaagcgtaat tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat
   103141 tctgagcacg gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca
   103201 acgtaatatg tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga
   103261 tggttcaggt aaatcgaccc tcacggcaag cctggtaaat gaactggcag caagaatgcc
   103321 aacagaacac atttatctcg ggcaatcgtc cgggcgaatt ggcgaatgga tttcacagct
   103381 ccctgttatt ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga
   103441 aaagccctca acaccgcctg gcaatattac tgcactggtt atctatctgc tttcctgctg
   103501 gcgggcgtac aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac
   103561 cgaccgctac ccgcaggttg aagtgccggg gtttcgcttt gatggcccgc aattggcaaa
   103621 aaccacgggc ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca
   103681 atggatggca tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc
   103741 gtttgcgcgt aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc
   103801 gcaactgaca ttcaatggcg caaagatcct tgaactggat gggcgacacc ccgccgatga
   103861 aattctgcaa gcgtcacaac gcgcaattca cgccgcgctt tcctgatcac caaaatggat
   103921 aggggatgat ttaacgaatg gatgcactac aaactcaaac tgttaatagt acaaccgcgc
   103981 cgcagcccaa ctacattccg gggctgattg cggtggtcgg gtgtgatggc accggtaaat
   104041 ccacactgac caccgacctg gtgaaatcgc tgcaacaaca ctggcaaacc gagcggcgct
   104101 atctggggct gctctccggc gaagacggcg acaaaatcaa acgattgccg ttggttggcg
   104161 tctggctgga acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat
   104221 ctccggcgct atgggcggcg gtgattatgt actgcttctc gctgcgaaga atggcgaatc
   104281 tacgcaaggt tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc
   104341 aggctgaaat ttcgggcttt tattatgatg gaccggggat tggcgtcgaa cgtgcgaccg
   104401 ggaaaatcag catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat
   104461 atcgcccgga attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc
   104521 ctgaccatga ctatgccgag ctgcaggaca aaatcggtgt catgtcgaag attggctata
   104581 acggcacaaa aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg
   104641 cacagaaggc ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct
   104701 ggcgggatac tttttactat caacgataag gtcaggctga attggcgggt tttaacatca
   104761 aacattggtt tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct
   104821 ccagtaatgt cgtgagcgcc ttgctgggtc tgttggcgct ctcgtgtgcc ggtaaaggga
   104881 tgacgcccgc catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg
   104941 attttattaa gtttcagaca tggcaactgg tggttcagta cggaacacca gcattaacca
   105001 acaataatcc gcagcaattc cgcaatgtcg tctcattttc cttctcgctg gatatcgtca
   105061 gcggcgcggt ggcgattgtc ggtggcattg ccttactgcc attcctttcc cattcattag
   105121 gtctggatga ccaaagtttt tggctggcag cgctctattg cacgctcatt ccttcaatgg
   105181 cttcctccac gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac
   105241 agcaggcgac gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact
   105301 ttggttttgc gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt
   105361 actggtggtt tgccgcgcgc gaattacgcc gccgaaatat ccataacgcc ttcaaattga
   105421 atctgtttga gtctgcccga cacattaaag gcgcgtggag ttttgtctgg tcaaccaaca
   105481 ttgcccactc catctggtcg gcgcgtaact cgtgcagcac agtgttggtg gggatcgtgt
   105541 taggacccgc tgccgccggg ttatttaaaa tcgccatgac attcttcgac gccgccggaa
   105601 cgccagcggg tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca
   105661 ccaccagacc gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc
   105721 tggtggcgct cgcggtgttg atcgtcggca agccgctcat ttcgctggtg tttggcgtta
   105781 agtatctcga agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc
   105841 tgggcttccc tcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg
   105901 cgcaaaccat cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg
   105961 gtgtgctggg agcagcgttt gcttacttcg gtgggcaatg tctggatgtg gtgttctcgc
   106021 taattccgac cttaaaggca ttttttcagc gccattcctt gctttataac gcagccggag
   106081 agaaatcctg atgatgagaa aatatttccc gcttgaggcg agcgagcgtc tatttgttgc
   106141 gattgaagaa gatgatgtcg ttgatgcaca ggtcagtttg ccgccgacta tcgcactaag
   106201 ttgtaccacc gaaatcatcc atgataatta cgcgttatgc ctgaaattct ggctggatgg
   106261 tgttaatcgt caggagctac tgcgtttaat ccgcaaacag gcgaaaggcg atgagctgac
   106321 gacagacgag cgcaaaaagt ataaatacat gcgagcgcgc tacaagcatc tgcgctttgc
   106381 gcaacggctg tatttaaaga agcatcaggc cggattttta ttcggcaaaa cgactgtttt
   106441 tttggggcgt tttcaggacg gctttcgtaa cggtaagaaa aatattgtgt cgtattacgg
   106501 caacctgtta cgggtttatt taagctctcc ggtctggtcg ttggtgaact attcattacg
   106561 ccatagccag ttagaaagcg taagcggttt tattgcctat cggcaaaagc aaatgcatac
   106621 cctgaaagag ataattgcca aaccgcgact aactggcagg gaatttcatg atgtgcgaaa
   106681 aatcatcagc cagcaggttt cttattacga caccctaaga tcgcttgatc ctgaaaataa
   106741 agaggcgtta cagatttccc gcttcctcgc cgccattaat ggcctgatgg gggataaaca
   106801 cgacgacatg gtggcggatg atatggaaaa ccgccagtcg tatgatgcac cgatggcgct
   106861 ggatagcgat attcgtcagc gcttagaact gctgatttcg cgttttccgc tataaccgga
   106921 gcaatgatgt catctcgttt gattattgcg ctgattatca tgctcttagc cccgggtgtg
   106981 caggcgcaca atttcgtcac tggtaaaaca gttacccctg tttatatcca ggaaggcggc
   107041 gagctgcttc tgaatagcga cgacgaaatt cactaccaaa agtggaacag cacacagttg
   107101 gcgggaaaag tccgcattat tcagtatatc gccgggcgta agtcggcgaa gaaaaagaac
   107161 tcactgttaa ttaaagcggt tgaagcggca aactttccgc aggatcgttt tcagcccacc
   107221 accattgtga acaccgatga tgccatcttt ggtaccggct attttgtcgt tggcaaaatc
   107281 gaaaaaaata aacgccgcta tccatgggcg caattcgtta ttgacggcaa tggacaggga
   107341 cgtgttgcct ggcacttgca ggagcagagt tcaaccattc tggtgctgaa taaatacggg
   107401 caaattcagt gggcaaagga cggcagcctg acgccggaag aggtagatca tgtcattgcg
   107461 ctggcgcaga aactgatcaa tgaataaaaa attcaaagga ttgagggcgt tagcgagatg
   107521 aagctggttg aacactacat catgcgtggt acgcgccgtc tggtgttgat tatcgtcggt
   107581 tttctgatct ttattttcgc cagctactcc gcacagcgtt atctgaccga agcggcaaat
   107641 gggacattag ctcttgatgt tgtgctggat atcgttttct acaaagtgct gattgcactg
   107701 gagatgttgt tacctgttgg actgtatgtg tcagttggcg tgacgctagg gcagatgtat
   107761 accgattcgg aaattaccgc tatttctgcg gcggggggca gtccgggacg cttgtacaaa
   107821 gccgttcttt atctggcgat accgctaagt atttttgtca cccttctgtc gatgtatggt
   107881 cgtccgtggg cttatgcgca gatttatcaa ctggagcaac agtcacagtc ggagctggat
   107941 gttcgccagt tgcgggcaaa gaaatttaac actaacgata acggacgaat gatcctttcg
   108001 cagacggttg atcaggataa taatcgcctg actgacgcgc tgatttatac ttctactgcc
   108061 aatcgaaccc gcattttccg cgcccgttcg gttgatgtgg ttgacccatc acctgagaaa
   108121 ccgaccgtta tgttgcataa cgggaccgcc tatcttctcg atcatcaggg gcgtgacgac
   108181 aacgaacaga tctaccgtaa tctgcaatta catctgaatc cgctggatca aagccctaac
   108241 gtcaaacgca aagcaaaatc ggtcacggag ctggcgcgct ccgcctttcc tgccgatcat
   108301 gccgaactgc aatggcgaca aagccgtggc ctgacagcgt tgttgatggc gctgctggcc
   108361 atttcattaa gtcgggtaaa accgcggcaa gggcgatttt caacgttatt gccactgacg
   108421 ttgctgtttg ttgccatttt ttatggcggc gacgtctgcc gtacgctggt ggctaacggc
   108481 gcgattcccc tcattcctgg tttgtggtta gtacccggac tcatgctaat gggcctgctg
   108541 atgctggtcg cacgcgactt ctctttgctg cagaaatttt cccgatgaat gttttcagcc
   108601 gctatttaat ccgtcatctc tttctcggtt ttgccgccgc cgcagggcta ttgctgccgc
   108661 tttttaccac cttcaacctg attaacgaac tggatgatgt cagcccgggc ggttatcgct
   108721 ggactcaggc ggtgctggtg gtgctgatga ccttaccgcg cacactggtc gaactttcgc
   108781 cgtttatcgc cttattggga gggattgtcg gcctggggca actatcgaaa aacagtgagc
   108841 ttaccgccat tcgcagcacg gggttttcta tcttccgtat tgcactggtg gcgctggttg
   108901 ccgggatatt gtggactgtt tcgttaggcg cgatagatga gtgggtggcg tcgccattgc
   108961 agcaacaggc gctgcaaatc aaatcgactg ccactgcgtt gggggaggac gatgacatta
   109021 ccggcaatat gctgtgggcc aggcgtggta atgaatttgt gacggtgaaa tcgctgaacg
   109081 agcagggcca gccggtgggc gtggagatat ttcattaccg cgacgatctc tcgctcgaat
   109141 cctacattta tgcacgcagt gccaccattg aagacgacaa aacgtggatc ctgcatggtg
   109201 tgaatcataa aaaatggctg aatggcaaag aaacgctgga aacattagat aatcttgcct
   109261 ggcaatcggc cttcaccagt atgaatcttg aagagttatc aatgccgggc aatacttttt
   109321 ctgtccgtca gcttaatcat tacatccatt atttgcagga aaccgggcaa cccagcagcg
   109381 aataccgcct tgcactgtgg gaaaaactgg ggcaacccat cctgaccctg gcgatgattt
   109441 tgctggctgt gccgtttacc tttagtgccc cgcgttcgcc tgggatgggt agccgtctcg
   109501 ctgtaggcgt catcgttggc ttactcacct ggatcagcta tcaaatcatg gtcaatctgg
   109561 cattgctatt tgcgttgagc gcacctgtta ccgcgctcgg tttaccgata gtgtttgtgc
   109621 tggtggcgtt gagcctggtg tattggtatg acagtcaaca ttaaacccgc cgtggcgcag
   109681 caccacagcg ggctcacatt actgcttcag agtagcaaat gcctgaatga tggcatcgat
   109741 ttgctcacga ctgtgggcag cgtttacgct acagcgcagc agcgtgatgc ctgcaggcgc
   109801 ggcaggtggc aggataagat tcacataaac gcccagcgaa ataagctcgc gccagatgcg
   109861 taaaccttcc tctttcgaac cgattattac tggcactacc gggctaatat gtgaacttaa
   109921 ctcataaccc agtttttgca gaccgtcata cagatggttg gcgttatcca tcagcttttg
   109981 ccgcagctct ggttgtgagg caatggttct taacgacgag cgcacggtcg caatgcagga
   110041 cggagaagga gaggcggtaa aaatatacgg gcggctgccg gagcgcagca cttccatcgc
   110101 ttccgaaccg acagcaaaac cgccaatcga ggccagactt ttactgaagg taccaacgat
   110161 aatatccaca tcatcttcaa cgccgacagc ttcagccagt ccacgccccg ttgcacctaa
   110221 cacgccaaac gaatgcgctt catcgacaat caaataacca ccgagacgac gtttgatatc
   110281 aacaatttcc gccagcggcg caacgtcacc gagcatgctg tagatgccct caacgatgat
   110341 aatcgcctct ttcgcccgtt caccgaggcg aaccatgcgc cgttccagat ctttagcatc
   110401 gttatggcgg aaacgaataa tttccgcgcc accgagagaa caggcgtcat aaatactggc
   110461 gtggctgtcg gcatccagta acaccacggc gttatggtcg gcaagtgcgc tgatgacgcc
   110521 taaattagcg gtataaccgg ttgaaaaaac gatagcggta gggcgattaa aaaactcggc
   110581 gatctctttc tctaacgcca gatgcggcgc ataactgccg tttgccatac gagaaccggt
   110641 ggtgccggtg ccttgggccg ctaatgctgc ctgaccttcg gcgatggcat catgattaaa
   110701 ggttaaaccg agataattat tggtcccggc cagaatcact tttttattgc cgatccgacc
   110761 ttcggtggcg gaatacactt catcaataca ggtgccaaac ggtgttaacc cgttgtcact
   110821 aaattgcagt cgctcgccag caaggcgcgc aaatttatcg tatagcccca ttatttattc
   110881 tccagccatg gaagtagtgt ttccattaat tgcgcagggg ttttgacatc cagcagaata
   110941 ttaataggaa tggaaatatc gaatctgtct tccagcatca ttaagagatc catcactttg
   111001 atcgactcca ggccaagatc gttgacgaga tcgctgtcgg gcttaatttc cacaccgttt
   111061 tcgaccaaat cctgtaaaca ggaaaggatg taatccatta ctatttcacg atttaccata
   111121 gaaacgttaa cacaccttta aacaagacaa tcaaaatagc ccgttgcgta atgcgtgttc
   111181 caggctaatc ccgggaaacc aattaatatc ttcagaaata cggttattat ttgccgacca
   111241 gtcggcgtgg gttaattcac ggattttcga gcgggtcagc ataggttctt tacctgccag
   111301 gcgactcaac gcggtactga tatccgcaag gcaggtgagt accgacagag gaataccaac
   111361 cattcgaacg gaaccgcaac gggcgtcggc ggcaagctgt cttacgcgtt gccagtcata
   111421 gccgccggcg acgccatcgc ataattcata ggtttgcgtc tgtacagttt cggcgcttaa
   111481 ccactgcccc acggcttgcg cgaaatcggt gacgtgaaga aaagagagct gtgtctctgg
   111541 cgtgccaagt cgtggcagca ggccgcgcag catccagtca aacagcggtt ttaactcttt
   111601 atcgccggga ccatacacgg ctgtcgggcg aaaaacgccc agcgtaattt catcagccat
   111661 tgcagtcagc cgttgttcgg cgacgtgttt ggaatttgcg taccaggaga gttcgggatg
   111721 gcgcgccgcc agcgaagaga taaacagaaa acgttggcaa aagccgctct cttttgccgc
   111781 ttgcatcagt cgcaggctgc cgtcaacgtt acagcgggta aaaatctctt ctttgtgccc
   111841 gcgcacttgc ccggcgcaat ggaccaccgc gctggctccg gcaaccagtt cgctaagtga
   111901 atgagtatct tccagcgaac cgcgcaccca gatgagatta tcattgacgt gagcgcgggc
   111961 agtacgcgtc aatgcgcgaa cgtgaaagcc gcgggcgagc aggttatcga taatatattt
   112021 accgataaac ccggtagcgc ccgtcaccgc gacagtttga ttcatgccag ggattcctgc
   112081 acatgaaggc ttgcagcata agccttctga taacgttttt tcgcttccgc acgggcaggc
   112141 ttgccggagg acgttcgggg aatactgtgg ggcggcaaca gatcgatagc cgcggtcacg
   112201 ccaaattcgc tttggatccg tgctgccagc gcgtggataa gctgcccgcg acgttcttca
   112261 tcgctgatcc gacactggat ctgcaaaatg attttttcct gggcggtaac aaaagcaatc
   112321 gcatcgccag aatgaatttc cggttcctgt tccgcaatgt attcaatatc ctgcggccag
   112381 atattacggc cacgaataat aatcagatct ttaatgcgtc cggtgacata cagataaccg
   112441 tccagcagat aaccgaggtc gccggtgtct aaccagcccg tcgcggcaat ctcgtcttgt
   112501 gaaatctggt cgccaaagta accgctcatc aggctagggc cggagatgca aatatggcct
   112561 accacacgtt ccgcgacagg tatacctgct tcattgcgga tttcaatgcc atgttccggc
   112621 aacgctttac cgcagttgac gaaagtcgat acggcgcgtg tttcagcatc cggcgcgacg
   112681 gctttgccct gatattcaag gatgtcgcga tccacttcgt taaccacaac cccggaagct
   112741 tcatcagaga agctgacagc cagcgcattt tctgccagtc cgtagcacgg catgaaagtt
   112801 ttatcgtcaa agttgacctg acggaaacat tcagcgaatt gatggagttg ttctgcggag
   112861 atgggttctg cgccaatacc agcgacgcgc cagcaggaaa gatccagttc agcgagatct
   112921 ttttcattca cgcggcgctg gcacaattca tagccaaacg gcggcgcaac ggaaacggtg
   112981 ccgcgatttt tactgatcaa tttaagccat tgcagaggac gcatggcaaa atcctgagtg
   113041 cgcaaataat ctactgaaag ctgcgtggcg acgggggtca ggagaaagcc gaccagtccc
   113101 atatcatggt agaaaggcag ccaggagacg cagcggtcgc cagggcgtaa tttaatgcca
   113161 tcgtggctta tagcacgtag attagccatc acttcgcgat gggtgataat gacgccacgg
   113221 ggaaaacggg tgctgccgga ggtgtactgg aggtaggcga tatcgttcgg aactggacgc
   113281 tggagcgcaa catcggcttc cggtaatgcc ttaaaccagg cgtggcttaa aacatgtaat
   113341 tcggggttgt tgttatgcgt cgcggcattg accagcggta accactcatc accagtgata
   113401 atggctgcgg gctggcaact tgccagtaaa ccctgcagtt tggcgctcca ggaatcccgc
   113461 tgaccgacgc ccattggaat cgccaacggg acggcgacta agccggcata ctggcaggaa
   113521 aaaaaagcct ctacaaaccc gctactcgtt tcggcaatca gcgccacgcg atcgcctttt
   113581 ttcaggttca gcgataacaa ccgcttcgca ccagcttcgg cacgcgcttt taacgtctga
   113641 tattccagtt gatcttcaag ttggcaacgt ctgtcataaa aattcattcc ggcgctactc
   113701 agagcggcgt agtccaaagc atcaaccagc gttggaaaat cggcatagcg cataggtagg
   113761 gaatgcgtaa agattttatt agacatatac accacgaaaa aattggatat caaaaataaa
   113821 atgacaataa tgcggtggat tatataggca taaaagataa atgagatagt attttcatca
   113881 gtgtgaaaat gaacattgat ttaaataatg agtttgagtt tttttgtttt tattgtgaat
   113941 gatatttaat ttatgaatat attaagaaga agccttgtcc ggtcgcctta aatcgacaaa
   114001 aggcattgag gtgcttctgt gtagtctctg atcttatctt aattattaat aaatactatt
   114061 tattctttgt ttgtattcat tgggttgatt attaaggtgc cgttcgttgc tgttgcaacc
   114121 tgtttgtcac cgttaattat ttgttaccca cagaactatg gaatgcgatc tgctgatgat
   114181 taaaattgaa aaagtaatta ataaaaatga ccttaaggca tttatcgctt ttccgtcatc
   114241 actttatccc gacgatccaa actggatacc ccctttattc attgaacgca acgagcattt
   114301 gtctgcgaaa aatccgggga cggaccatat catctggcag gcgtgggtgg caaaaaaagc
   114361 ggggcagata gttgggcgta ttaccgcgca aatagatacc ttgcaccgcg agcgttacgg
   114421 tgaagatacc ggtcatttcg gcatgattga cgccattgat gatccgcagg tttttgccgc
   114481 gttgtttggc gcagcggaag cgtggttgaa gtcacaagga gcaagtaaga tcagcgggcc
   114541 tttcagcctg aatatcaatc aggaaagcgg attactgatt gaaggtttcg atacaccacc
   114601 ctgtgcgatg atgccgcacg gtaaaccgtg gtatgccgcg catattgaac aattgggtta
   114661 tcacaaaggc attgatttac tggcgtggtg gatgcagcga accgatctca ctttctctcc
   114721 ggcgctaaaa aaactgatgg atcaggtgcg taagaaggtg accattcgct gcatcaatcg
   114781 ccagcggttt gccgaagaga tgcagatcct gcgtgagatt ttcaactctg gctggcagca
   114841 aaactgggga tttgtgccgt ttaccgaaca tgaattcgcg accatgggcg atcaacttaa
   114901 atatctggtg ccggacgata tgatctacat cgctgagatt gattctgcac cctgcgcgtt
   114961 tattgtcggc ttgccaaaca tcaacgaggc gattgccgat ctgaacggat cgctctttcc
   115021 cttcggctgg gcaaaattgc tgtggcgttt aaaagtcagc ggtgtgcgaa ctgcgcgagt
   115081 accgctgatg ggcgtgcggg acgagtatca gtttagccgc atcggcccgg tgattgcgct
   115141 gttattgatt gaagccttac gcgatccgtt tgcccgccgg aagatcgatg cgctggaaat
   115201 gtcatggatc ctcgaaacca atacaggcat gaataacatg ctggaacgca ttggggcgga
   115261 gccgtacaag cgttaccgat tgtatgaaaa gcagatttaa taccagctaa ctctggttca
   115321 tctccagcgg caccgccgag cgaatcaaat ggtggccttc cagctcgata tcgtggagat
   115381 tctttttcac ggtccgcaca tgatcgcgtg ctgcgcgctg ggcgacgtgc ggcagccgct
   115441 gcaacaccgc gttgtagatc cgcgcatgct ggcgatcgat ctgctttttt tgctgtggtc
   115501 gatggtagag attatttacg ctggcaaaca ctgaattaaa catcaggtcg gtcaatgatt
   115561 gcagtgtaaa caccaacacc tgattgtgag aagcctgaca aatggcgaga tggaaagcgt
   115621 gatccaactg cgcatgttcg atcagcgaaa tctctttgtt gttctcactg gcggcgagca
   115681 ttttttcata acagcgggtt atcacaacaa aatcagcctg cgttcccagc gttgccgcca
   115741 gccttgccga ttcgccctcc agcaatgcgc gaacgtcgag cagatcatac agcgttcgcg
   115801 gctgcgtgct gaacagatga attagcgggc tggtgtcctg cacccgatta agccgtgcga
   115861 cacgagaatc gcgaccctgc gccgtttcaa taatcccgcg cccgcgcagc acggtcagcc
   115921 cttcacgcag tgcggagcgt gagaagccga gcttttcaca cagtcgacgt tccgagggaa
   115981 gcggctgacc gaccttcagt acgccgtcga taattaaccg ttcgatactc tctgcaacca
   116041 cttcgcaaat agggcgacgt tcatctttca ttcccggacc tcgtgcacag gtaggaccaa
   116101 tttttctgtt cttgactcgc aaaacacgca catcacgtaa gtgattatgt tacatcaatt
   116161 taacattaaa ttaaccaaga caaggtcaca gagctggaaa aaaaatggtc tgaccggtag
   116221 ctaaagagat agacgaaaac gaaaagcccg cttaataact gttcacagaa gcagcgcgca
   116281 aaaatcagct gccacacaac acaacaaagc gaagcctact catgagcatc ttgtacgaag
   116341 agcgtcttga tggcgcttta cccgatgtcg accgcacatc ggtactgatg gcactgcgtg
   116401 agcatgtccc tggacttgag atcctgcata ccgatgagga gatcattcct tacgagtgtg
   116461 acgggttgag cgcgtatcgc acgcgtccat tactggttgt tctgcctaag caaatggaac
   116521 aggtgacagc gattctggct gtttgtcacc gcctgcgtgt gccggtggtg acccgtggtg
   116581 caggcaccgg gctttctggt ggcgcgctgc cgctggaaaa aggtgtgttg ttggtgatgg
   116641 cgcgctttaa agagatcctc gacattaacc ccgttggtcg ccgcgcgcgc gtgcagccgg
   116701 gcgtgcgtaa cctggcgatc tcccaggccg ttgcgccgca taatctctat tacgcgccgg
   116761 atccttcctc acaaatcgcc tgttcaattg gcggcaatgt ggcggaaaat gccggtggcg
   116821 tccactgcct gaaatatggt ttgaccgtac ataacctgct gaaaattgaa gtgcaaacgc
   116881 tggacggcga ggcgctgacg ttgggatcgg atgcgctgga ttcgcctggt tttgatctgc
   116941 tggcgctgtt caccggatcg gaaggtatgc tcggcgtgac caccgaagtg actgtaaaac
   117001 tgctgccgaa gccgcccgtg gctcgggttc tgttagccag ctttgactcg gtagaaaaag
   117061 ccggacttgc ggttggtgac atcatcgcca atggcattat ccccggcggg ctggagatga
   117121 tggataacct gtcgatccgc gcagcggaag attttattca tgccggttat cccgtcgacg
   117181 ccgaagcgat tttgttatgc gagctggacg gcgtggagtc tgacgtacag gaagactgcg
   117241 agcgggttaa cgacatcttg ttgaaagcgg gcgcgactga cgtccgtctg gcacaggacg
   117301 aagcagagcg cgtacgtttc tgggccggtc gcaaaaatgc gttcccggcg gtaggacgta
   117361 tctccccgga ttactactgc atggatggca ccatcccgcg ccgcgccctg cctggcgtac
   117421 tggaaggcat tgcccgttta tcgcagcaat atgatttacg cgttgccaac gtctttcatg
   117481 ccggagacgg caacatgcac ccgttaatcc ttttcgatgc caacgaaccc ggtgaatttg
   117541 cccgcgcgga agagctgggc gggaagatcc tcgaactctg cgttgaagtt ggcggcagca
   117601 tcagtgggga acatggcatc gggcgcgaaa aaatcaatca aatgtgcgcc cagttcaaca
   117661 gcgatgaaat cacgaccttc catgcggtca aggcggcgtt tgatcccgat ggtttgctga
   117721 accctgggaa aaacattccc acgctacacc gctgtgctga atttggtgcc atgcatgtgc
   117781 atcacggtca tttacctttc cctgaactgg agcgtttctg atgctacgcg agtgtgatta
   117841 cagccaggcg ctgctggagc aggtgaatca ggcgattagc gataaaacgc cgctggtgat
   117901 tcagggcagc aatagcaaag cctttttagg tcgtcctgtc accgggcaaa cgctggatgt
   117961 gcgttgtcat cgcggcattg ttaattacga cccgaccgag ctggtgataa ccgctcgtgc
   118021 cggaacgccg ctggtggcga ttgaagcggc gctggagagc gccgggcaaa tgctctcctg
   118081 cgagccacca cattatggtg aagaagctac ctggggcggg atgatcgcct gcgggctggc
   118141 ggggccgcgt cgcccgtgga gcggttcggt ccgcgatttt gtcctcggca cgcgcatcat
   118201 taccggcact ggaaaacatc tgcgttttgg tggcgaagtg atgaaaaacg ttgccggata
   118261 cgatctctca cggttaatgg ccggaagcta cggttgtctg ggtgtgctca ctgaaatctc
   118321 gatgaaagtg ttaccgcgac cacgcgccac cctgagtctg cgtcgggaaa tcagcctgca
   118381 agaagccatg aatgaaatcg cccagtggca actccagcca ttacccatta gtggcttatg
   118441 ttacttcgac aatgcgttgt ggatccgcct tgagggcggc gaaggatcgg taaaagcagc
   118501 gcgtgaactg ctgggtggcg aagaggttgc gggtcagttc tggcagaaat tgcgtgaaca
   118561 acaactgccg tttttctcgt taccaggtac cttatggcgc atttccttac cctgcgatgc
   118621 gccgatgatg gatttacccg gcgagcaact gatcgactgg ggcggggcgt tacgctggct
   118681 gaaatcgaca gccgaggaca atcaaatcca tcgcatcgcc cgcaacgctg gcggtcatgc
   118741 gacccgcttt agtgccggag atggtggttt tgccccgcta ccggctcctt tattccgcta
   118801 tcaccagcag cttaaacagc agctcgaccc ttgcggcgtg tttaaccccg gtcgcatgta
   118861 cgcggaactt tgaggagcag gctatgcaaa cccgattaac cgaagatatg cggcagaacg
   118921 cgcgggcgct ggaagccgac agcatcctgc gtgcctgtgt tcactgcgga ttttgtaccg
   118981 caacctgccc aacctatcag ctcctgggcg atgaactgga cgggccgcgc gggcgcatct
   119041 atctgattaa acaggtgctg gaaggtaacg aagtcacgct taaaacacag gagcatctcg
   119101 atcgctgtct cacttgccgc aattgtgaaa cgacctgtcc ttctggtgtg cgctatcaca
   119161 atttgctgga tatcgggcgt gatattgtcg agcagaaagt gaaacgccca ctgccggagc
   119221 gaatgctgcg cgaaggattg cgccaggtag tgccgcgtcc ggcggtcttc cgtgcgctga
   119281 cgcaggtagg gctggtgctg cgaccgtttt taccggaaca ggtcagagca aaactgcctg
   119341 ctgaaaccgt gaaagctaaa ccgcgtccac cgctgcgcca taagcgtcgg gttttaatgc
   119401 tggaaggctg cgcccagcct acgctttcgc ccaacaccaa cgcggcaact gcgcgagtgc
   119461 tggatcgtct ggggatcagc gtcatgccag ctaacgaagc aggctgttgt ggcgcggtgg
   119521 actatcatct taatgcgcaa gagaaagggt tagcgcgggc gcgcaataat attgatgcct
   119581 ggtggcccgc gattgaagca ggtgccgagg caattttgca aaccgccagc ggttgcggcg
   119641 cgttcgttaa agagtatggg cagatgctga aaaacgatgc gttgtatgcc gataaagcgc
   119701 gtcaggtcag tgaactggcg gtcgatttag tcgaacttct gcgcaaggaa ccgctggaaa
   119761 aactggcaat tcgcggcgat aaaaaactgg ccttccactg cccgtgtacc ctgcaacatg
   119821 cgcaaaagct gaacggcgaa gtggaaaaag tgttacttcg tcttggattt accttaacgg
   119881 acgttcccga cagccatctg tgctgcggtt cagcgggaac atatgcgtta acgcatcccg
   119941 atctggcacg ccagctgcgg gataacaaaa tgaatgcgct ggaaagcggc aaaccggaaa
   120001 tgatcgtcac cgccaacatt ggttgccaga cgcatctggc gagcgccggt cgtacctctg
   120061 tgcgtcactg gattgaaatt gtagaacaag cccttgataa ggaataacaa aatgaaaact
   120121 aaagtcattc ttagccagca aatggcgagt gcaattattg ccgcaggtca ggaagaggcg
   120181 cagaaaaata actggtctgt ttccattgct gttgccgatg acggcggtca tctgctggcg
   120241 ttaagtcgca tggatgattg cgcgccaatt gcggcttaca tctcccagga gaaagcgcgt
   120301 accgccgcgc tgggacgtcg tgaaactaag ggctatgaag agatggtgaa caacggacgt
   120361 accgcgttcg tgactgcgcc gttactgacg tcgctggaag gcggcgtacc ggttgttgtg
   120421 gatgggcaaa ttattggtgc cgtgggcgtt tctggtttaa ccggagcaca ggatgcgcag
   120481 gtcgcgaaag cggcagcagc ggtgttggcg aaataagcga aaacgaggag ataaataatg
   120541 agtgaaacca taacccaagg ccttttacgc attgacgccg actttaaacg ttttgtggat
   120601 gaagaagttt tgccgggcgt agaactggat gctgccgcgt tctggcgcaa tgttgatgag
   120661 attgttcacg atctggcacc agaaaatcgt cagttgctgg cagaacgcga tcgcattcag
   120721 gcagcgcttg atgagtggca tcgcagcaat ccggggccgg taaaagataa agcggcctat
   120781 aaatctttcc tgtgtgaact gggctacctg gtgccgcaac cggagcgcgt gacggtggaa
   120841 accacgggca ttgacagcga aatcaccagc caggcggggc cacagctggt ggttccggca
   120901 atgaatgccc gctacgcgct gaacgcggcg aacgcgcgct ggggctcact gtacgatgcg
   120961 ttatacggca gcgacatcat cccgcaggaa ggggcgatgg tcagcggcta cgatccgcag
   121021 cgcggtgagc aggttatcgc ctgggttcgg cgtttcctcg atgaatctct accgctggaa
   121081 aacggcagct atcaggatgt ggtggcgttt aaggtggttg ataaacaatt acgcatccag
   121141 ttgaaaaatg gtaaagaaac cacgttacgc accccggcgc agtttatcgg ttaccgtggc
   121201 aatgccgctg cgccgtcctg cattttgctg aaaaataacg gcctgcatat tgagctgcaa
   121261 atcgatgcca acgggcggat tggcaaagac gatccggcgc atatcaacga tgttatcgtc
   121321 gaagcggcca tcagtaccat tctcgactgc gaagattcgg tcgcggcggt tgatgcggaa
   121381 gataaaatcc tgctgtaccg caacctgctg ggcctgatgc aggggactct gcaagagaaa
   121441 atggagaaaa acggtcggca aatcgtgcgt aaactgaatg acgatcgtca gtacaccgcc
   121501 gccgatggct ctgaaatttc tctgcacgga cgctcgttgc tgtttatccg caacgtgggg
   121561 catttgatga ccattcctgt gatttgggac agcgaaggca atgaaatccc ggaaggcatt
   121621 cttgatggcg tcatgactag cgcgattgcc ctctatgatt taaaagtgca gaaaaactcg
   121681 cgcactggca gtgtctatat tgtgaaaccg aaaatgcacg gtccgcagga agtggcgttc
   121741 gccaacaaac tgtttacccg cattgagaca atgctcggta tggcaccgaa taccctgaaa
   121801 atgggcatta tggatgaaga acgtcggacc tcgctgaact tgcgtagctg tatcgctcag
   121861 gcgcgcaacc gcgtggcgtt catcaatacc ggtttcctcg accgtaccgg cgatgaaatg
   121921 cattcggtga tggaagctgg cccgatgctg cgtaaaaacc agatgaaatc gacgccgtgg
   121981 atcaaagcct acgagcgtaa taacgtgctt tccggtctgt tctgtgggct gcgcggtaaa
   122041 gcgcaaattg gtaaaggcat gtgggcaatg ccggacctga tggcagacat gtacagccag
   122101 aagggcgacc aactgcgtgc cggggcaaac acagcctggg ttccgtcacc aaccgctgct
   122161 acgctccatg cgctgcacta ccaccaaacc aacgtacaga gcgtgcaggc caacattgcc
   122221 cagagcgagt tcaatgctga atttgaaccg ttgctggacg atctgctgac tattccggtg
   122281 gctgaaaacg ctaactggtc ggtggaagag atccaacaag agctggataa caacgtgcag
   122341 gggattctgg ggtacgtggt gcgttgggtg gagcagggga ttggttgttc aaaagtgccg
   122401 gatattcaca acgtggcgct gatggaagac cgcgcaacgc tgcgtatctc cagccagcat
   122461 atcgccaact ggttacgtca cggtattctg accaaagaac aggtgcaggc gtcgctggag
   122521 aatatggcga aagtggttga tcagcaaaac gctggcgatc cggcttatcg tccgatggcg
   122581 gggaatttcg ctaactcgtg tgcttttaaa gctgccagcg atttaatctt cctcggcgtg
   122641 aaacagccaa acggctatac cgaaccgtta ttacacgcct ggcgtttacg cgaaaaagaa
   122701 agtcattaat agcgtcaaaa aagccccggc aagttttatt gtcggggcta tgatagtaac
   122761 tgcggtagta catttatccc ggaaataacc ggagcgatta tcagggttta acaatattaa
   122821 atacccgtag cgcattacta gcgggcctaa aacaacaata tggacatctt aacgatactc
   122881 tgcgcctgcg tgtgcggaac agaaaccctt acccgatata cttgttatgc cagaggtagc
   122941 aattgcgtta gcagcgtact gttgctgtaa agtataacgc ctatatttta cagttcagtc
   123001 ctcttttgct tattacaacc ttaagtatat caagcatata aagataataa gagactgaac
   123061 aatatggtta cctggaccca aatgtatatg ccgatgggag gactggggct atccgctctg
   123121 gtcgccctga tcccgataat attcttcttc gttgcactcg cggtattacg tctgaaagga
   123181 catgtcgccg gagcaacaac ccttatatta tctattctga ttgcgatatt cgcctttaaa
   123241 atgccgattg atatggcatt tgctgctgcg ggctatggct ttatttatgg attatggcct
   123301 attgcgtgga ttattgttgc ggcggtgttc ctgtataaat taaccgttgc cagcggtcag
   123361 tttgatatta tccgcagctc ggttatctcc atcactgacg atcagcgttt gcaggtgtta
   123421 ctgattggtt tctcctttgg cgcgctgctg gaaggggcgg ctggctttgg tgcgccggtg
   123481 gcgattaccg gtgcgctgct ggtgggcctg ggcttcaaac cgttatacgc ggcggggctg
   123541 tgtctgattg ccaacactgc gccggtggcg tttggtgcgt tgggcgtgcc gattctggtc
   123601 gccggtcagg taacgggaat cgatccgttc cacattggcg caatggcggg acgtcagtta
   123661 ccgttcctgt cggttcttgt gccgttctgg ctggtagcaa tgatggacgg ctggaaaggg
   123721 gtgaaagaga cgtggccagc ggcgctggtt gctgggggaa gcttcgctgt cacgcagttc
   123781 tttacctcta actatattgg tccggaactg ccggatatta cttcggcgct ggtgagtatc
   123841 gtctcactcg ctttattcct taaagtctgg cggccgaaaa ataccgaaac ggcaatcagc
   123901 atgggacaat ccgcatgtgc gatggtggtg aataaaccat cttctggcgg tcccgtgcct
   123961 tcagaatata gtctggggca aatcattcgt gcgtggtcac cgtttttaat cttaacggtg
   124021 ttggtcacca tctggaccat gaagccgttt aaagcgttat ttgctccggg cggcgcgttt
   124081 tattcactgg tgattaattt ccagatccct catttgcatc aacaagtctt gaaagcggcc
   124141 cccattgtcg cccagccgac gccaatggac gcggtgttta aattcgaccc cctctctgct
   124201 ggtggcaccg ctatttttat tgcggcgatt atctctatct tcatcctcgg cgtggggatc
   124261 aagaaaggta ttggcgtctt tgccgaaacg ctaattagtc tgaagtggcc gatactgtcg
   124321 attggcatgg tgctggcatt tgccttcgtc accaactatt ccggtatgtc caccacgctg
   124381 gcgctggtac tggctggtac aggcgtgatg ttcccattct tctcaccgtt tctcggctgg
   124441 ctgggcgtat tccttaccgg ctcggacact tcttcaaacg ccctgtttgg ttcactgcaa
   124501 tcgaccacgg cgcagcaaat caacgtctct gacaccctgc tggtggcagc aaacaccagc
   124561 ggcggcgtaa ctggcaagat gatctccccg caatctatcg ccgtggcctg cgccgcgacg
   124621 ggcatggtgg gtcgagaatc tgaactgttc cgctacaccg tgaagcacag tctgattttt
   124681 gccagcgtta tcggcattat cacgctgttg caggcgtatg tgtttaccgg gatgttagtt
   124741 tcgtaagtca ataaagccgg atgatcgtta atatcatccg gcttcgttgt gcacagaaaa
   124801 cccccagcta ggct
//
LOCUS       DAPOZO010000002       103985 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPOZO010000002 DAPOZO010000000
VERSION     DAPOZO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 103985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 103985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..103985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.002"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..6464)
                     /gene="air"
                     /locus_tag="ROI21_000117"
     CDS             complement(<1..6464)
                     /gene="air"
                     /locus_tag="ROI21_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin EaeX/Air"
                     /protein_id="HDX5109104.1"
                     /translation="MAGKAHGNGDRRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINPAHSDTAASLILPNVKTIPYTLGALESPPTVAARFGITVDELRRLNQFRTFA
                     RGFDHVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEVAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVLSQHTLHRTDDRTQTNHGIGWRYFTSSWMSGVNMFIDHDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDNDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSADGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEKPTLSLADSTLSVDLQILLADGKSTSMLTYTARDSS
                     GKPIPGMTLKTQAKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNGNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGAAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAEINGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSGVVEVSGTDKNEMGNWSEESDGVYTTTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTTYTAGGAIKVTVTL
                     KDSYENLVGGQRDAINQAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SGWASALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSADIRTDAHGMAEVDLSSTVADASTVEAKVNQSSDSKT
                     VNFVADVSTAQVAELVVTQDGSVADGSTANMLRVRVTDVFGNVLAGQTVSVTADNSAT
                     VAPTVITGPDGTVEISVTSQTAGTSAITASINNSSLSRNVTFVADVRTAKIADLVVTR
                     DNSVADGAMANTLRVRVTDAFGNTLAGQTVSVMADNSATVSPTVTTEPDGTVEISVTS
                     QTAGVSTVTASINSSSQSRNVTFVADVRTAKIADLVVIKDGSEADGSTANTLRARVTD
                     AFGNTLGGQTVSVLADNGATVAPTVTTQPDGTVEISVTSQTAGTSTVTASINNSSLSQ
                     NVTFVADVRTAKIADLVVIKDGSVADGATANTLQVKVTDAFGNALNGQTVSVMAGNGA
                     TVTPTVITGPDGTVEISATSQTAGISTVTVTINNSSLSRNVMFVADVRTAQIADLVVI
                     KDGAVADGAMANMLQVKVTDAFGNTLAGQTVSVLAGNGATTASTVTTQPDGTVEISVT
                     SQTAGTSVVTASINNSSQSRNVTFIADVRTAKIADLEVIKDGSEADGSTANTLRARVT
                     DAFGNALAGQTVSVLADNGATVALTETTKPDGTAEISVTSQTAGVSAVTVSINNSSQS
                     RNVTFIADVRTAQIAELVVIKDGSEADGSTANTLRVRVTDAFGNALAGQTVSVLADNG
                     ATVAPTVTTQPDGTVEISVTSQTAGTSTVTASINSSSQSRNVTFIADVSTAQIADLVV
                     IKDDSVADGAMANTLRVRVTDAFGNALAGQTVSVMAGNGATVAPTVITEPDGTVEISV
                     TSQTAGISAVTASINSSSQSRDVTFIADVRTAKIAELEVIRDNAVADGSTANTLQVKV
                     TDANDNTLAGQAVSVLAGNSATVASTVTTKPDGTVEISVTSQTAGTSTVTASINSSSL
                     SRNVTFVADVSTAKIADLVVIQDNSVADGAMANTLRMRVTDAFGNTLGGQTVSVTADN
                     SAMVASTVITGPDGTVEISVTSQTAGISIVTASINNSSLSRDVTFVADVRTAQIADLV
                     VIKDGSEADGLTANTLQVRVTDA"
     gene            complement(6666..7850)
                     /gene="nepI"
                     /locus_tag="ROI21_000118"
     CDS             complement(6666..7850)
                     /gene="nepI"
                     /locus_tag="ROI21_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDX5109105.1"
                     /translation="MSEFIAENRGADVITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            8084..8437
                     /locus_tag="ROI21_000119"
     CDS             8084..8437
                     /locus_tag="ROI21_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDX5109106.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            8421..8744
                     /locus_tag="ROI21_000120"
     CDS             8421..8744
                     /locus_tag="ROI21_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDX5109107.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(8860..9312)
                     /locus_tag="ROI21_000121"
     CDS             complement(8860..9312)
                     /locus_tag="ROI21_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDX5109108.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(9365..10699)
                     /gene="adeQ"
                     /locus_tag="ROI21_000122"
                     /gene_synonym="yicO"
     CDS             complement(9365..10699)
                     /gene="adeQ"
                     /locus_tag="ROI21_000122"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDX5109109.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGQFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            10874..12640
                     /gene="adeD"
                     /locus_tag="ROI21_000123"
     CDS             10874..12640
                     /gene="adeD"
                     /locus_tag="ROI21_000123"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDX5109110.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKALNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(12687..14078)
                     /gene="uhpT"
                     /locus_tag="ROI21_000124"
     CDS             complement(12687..14078)
                     /gene="uhpT"
                     /locus_tag="ROI21_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDX5109111.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(14334..15653)
                     /gene="uhpC"
                     /locus_tag="ROI21_000125"
     CDS             complement(14334..15653)
                     /gene="uhpC"
                     /locus_tag="ROI21_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDX5109112.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(15663..17165)
                     /gene="uhpB"
                     /locus_tag="ROI21_000126"
     CDS             complement(15663..17165)
                     /gene="uhpB"
                     /locus_tag="ROI21_000126"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDX5109113.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNIWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGCGQQGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(17165..17755)
                     /gene="uhpA"
                     /locus_tag="ROI21_000127"
     CDS             complement(17165..17755)
                     /gene="uhpA"
                     /locus_tag="ROI21_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDX5109114.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(17917..19380)
                     /locus_tag="ROI21_000128"
     CDS             complement(17917..19380)
                     /locus_tag="ROI21_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109115.1"
                     /translation="MVSVTSYSYPTETENDNSQFQSQSQGLSQSLRDSIEGNIAAFDA
                     MEQSGLLDNCRTLSKDATFREVFRNILLYILDSFHIYRHDISGKLVSYFHVTPESANA
                     NYKTWIATTGMDSYIKIEQTGSDKFKVTDVYRELNGKSLEKKSRDRDFSGRNVKEFIG
                     LLSEHDVLEKYQLSKVTVAANALKLEVVNNNFKNELSSGVTDAEHQLKKALYEQTQST
                     PDKSIQYELIPDKLIPDDCSYKSDIKKLDVREGEFSALLADASRHVGEASSFIACCGV
                     KINSTIHELLRSKTMALSGHGGVRKYKDFEKFFSEIVFTPDECNIVRNSLQKDGVEKK
                     TRENIISCLSSISGIGNLYYACSQAFPVALDRGDINAIQASNSISAGFNSNPGFCALM
                     GKTNFLFADFSLHTFTQTHYIVKAEINEAGNDFNIANFIYRTYGEDYIHNDSVDDSLN
                     ERSAEDYRFLDDSLLPENDFRNRRRNFSGNIAIATYK"
     gene            complement(19660..20142)
                     /locus_tag="ROI21_000129"
     CDS             complement(19660..20142)
                     /locus_tag="ROI21_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109116.1"
                     /translation="MVKQLCFDVNDCAEVRELLKQSNGNITDDKIDEIIACLASPQGI
                     TCFYYSSAQGYQTRLQHTSQDVRELLSKSLDGATSLTLHLKKSGNAFFGSGKYLNEMD
                     FTINGFSQKMYSMAVNNVGNEVKLQCSRYFTTGRVDEQNSKIDKVTFTFFGAATTADL
                     "
     gene            complement(21220..21510)
                     /gene="ilvN"
                     /locus_tag="ROI21_000130"
     CDS             complement(21220..21510)
                     /gene="ilvN"
                     /locus_tag="ROI21_000130"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDX5109117.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(21514..23202)
                     /gene="ilvB"
                     /locus_tag="ROI21_000131"
     CDS             complement(21514..23202)
                     /gene="ilvB"
                     /locus_tag="ROI21_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDX5109118.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGSDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(23308..23406)
                     /gene="ivbL"
                     /locus_tag="ROI21_000132"
     CDS             complement(23308..23406)
                     /gene="ivbL"
                     /locus_tag="ROI21_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDX5109119.1"
                     /translation="MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            23971..24060
                     /gene="tisB"
                     /locus_tag="ROI21_000133"
     CDS             23971..24060
                     /gene="tisB"
                     /locus_tag="ROI21_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDX5109120.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            24340..25524
                     /gene="emrD"
                     /locus_tag="ROI21_000134"
     CDS             24340..25524
                     /gene="emrD"
                     /locus_tag="ROI21_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDX5109121.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(25532..26029)
                     /locus_tag="ROI21_000135"
     CDS             complement(25532..26029)
                     /locus_tag="ROI21_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDX5109122.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHLLCPHAREEMPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(26026..26388)
                     /locus_tag="ROI21_000136"
     CDS             complement(26026..26388)
                     /locus_tag="ROI21_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDX5109123.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(26378..26725)
                     /locus_tag="ROI21_000137"
     CDS             complement(26378..26725)
                     /locus_tag="ROI21_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDX5109124.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(26785..28278)
                     /locus_tag="ROI21_000138"
     CDS             complement(26785..28278)
                     /locus_tag="ROI21_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDX5109125.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKS
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILLGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(28275..29990)
                     /locus_tag="ROI21_000139"
     CDS             complement(28275..29990)
                     /locus_tag="ROI21_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDX5109126.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPIFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFLKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            30157..31050
                     /locus_tag="ROI21_000140"
     CDS             30157..31050
                     /locus_tag="ROI21_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDX5109127.1"
                     /translation="MNGNLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEEIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(31047..32369)
                     /locus_tag="ROI21_000141"
     CDS             complement(31047..32369)
                     /locus_tag="ROI21_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDX5109128.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKHMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDDLLEANKAYWPELR"
     gene            complement(32369..33985)
                     /locus_tag="ROI21_000142"
     CDS             complement(32369..33985)
                     /locus_tag="ROI21_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDX5109129.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            34281..34997
                     /locus_tag="ROI21_000143"
     CDS             34281..34997
                     /locus_tag="ROI21_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDX5109130.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(34994..36655)
                     /locus_tag="ROI21_000144"
     CDS             complement(34994..36655)
                     /locus_tag="ROI21_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDX5109131.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(36851..37279)
                     /gene="ibpB"
                     /locus_tag="ROI21_000145"
     CDS             complement(36851..37279)
                     /gene="ibpB"
                     /locus_tag="ROI21_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDX5109132.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(37391..37804)
                     /gene="ibpA"
                     /locus_tag="ROI21_000146"
     CDS             complement(37391..37804)
                     /gene="ibpA"
                     /locus_tag="ROI21_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDX5109133.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(37798..37870)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            38110..38442
                     /locus_tag="ROI21_000147"
     CDS             38110..38442
                     /locus_tag="ROI21_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDX5109134.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(38444..39658)
                     /locus_tag="ROI21_000148"
     CDS             complement(38444..39658)
                     /locus_tag="ROI21_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDX5109135.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELSPTLDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKVAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPHQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            39759..40823
                     /gene="cbrA"
                     /locus_tag="ROI21_000149"
     CDS             39759..40823
                     /gene="cbrA"
                     /locus_tag="ROI21_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDX5109136.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRYLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFATLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLERISYALDSAEILRSVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(40820..42157)
                     /locus_tag="ROI21_000150"
     CDS             complement(40820..42157)
                     /locus_tag="ROI21_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5109137.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(42232..43380)
                     /gene="dgoD"
                     /locus_tag="ROI21_000151"
     CDS             complement(42232..43380)
                     /gene="dgoD"
                     /locus_tag="ROI21_000151"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDX5109138.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(43377..43994)
                     /gene="dgoA"
                     /locus_tag="ROI21_000152"
     CDS             complement(43377..43994)
                     /gene="dgoA"
                     /locus_tag="ROI21_000152"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDX5109139.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDVLARMGCQLIVTPNIHSEVIHRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(43978..44856)
                     /gene="dgoK"
                     /locus_tag="ROI21_000153"
     CDS             complement(43978..44856)
                     /gene="dgoK"
                     /locus_tag="ROI21_000153"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDX5109140.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQSTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPLQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(44853..45542)
                     /gene="dgoR"
                     /locus_tag="ROI21_000154"
     CDS             complement(44853..45542)
                     /gene="dgoR"
                     /locus_tag="ROI21_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDX5109141.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDLRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESSLNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            45820..46476
                     /locus_tag="ROI21_000155"
     CDS             45820..46476
                     /locus_tag="ROI21_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109142.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGGEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKKFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(46520..47332)
                     /gene="yidA"
                     /locus_tag="ROI21_000156"
     CDS             complement(46520..47332)
                     /gene="yidA"
                     /locus_tag="ROI21_000156"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDX5109143.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(47447..47845)
                     /locus_tag="ROI21_000157"
     CDS             complement(47447..47845)
                     /locus_tag="ROI21_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDX5109144.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(48085..50499)
                     /gene="gyrB"
                     /locus_tag="ROI21_000158"
     CDS             complement(48085..50499)
                     /gene="gyrB"
                     /locus_tag="ROI21_000158"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDX5109145.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHANAEQNLFEPVVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(50528..51601)
                     /gene="recF"
                     /locus_tag="ROI21_000159"
     CDS             complement(50528..51601)
                     /gene="recF"
                     /locus_tag="ROI21_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDX5109146.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(51601..52701)
                     /gene="dnaN"
                     /locus_tag="ROI21_000160"
     CDS             complement(51601..52701)
                     /gene="dnaN"
                     /locus_tag="ROI21_000160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDX5109147.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(52706..54109)
                     /gene="dnaA"
                     /locus_tag="ROI21_000161"
     CDS             complement(52706..54109)
                     /gene="dnaA"
                     /locus_tag="ROI21_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDX5109148.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            54406..54486
                     /locus_tag="ROI21_000162"
     CDS             54406..54486
                     /locus_tag="ROI21_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109149.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            54716..54856
                     /gene="rpmH"
                     /locus_tag="ROI21_000163"
     CDS             54716..54856
                     /gene="rpmH"
                     /locus_tag="ROI21_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDX5109150.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            54873..55232
                     /gene="rnpA"
                     /locus_tag="ROI21_000164"
     CDS             54873..55232
                     /gene="rnpA"
                     /locus_tag="ROI21_000164"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDX5109151.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            55196..55453
                     /gene="yidD"
                     /locus_tag="ROI21_000165"
     CDS             55196..55453
                     /gene="yidD"
                     /locus_tag="ROI21_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDX5109152.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            55456..57102
                     /gene="yidC"
                     /locus_tag="ROI21_000166"
     CDS             55456..57102
                     /gene="yidC"
                     /locus_tag="ROI21_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDX5109153.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            57208..58572
                     /gene="mnmE"
                     /locus_tag="ROI21_000167"
     CDS             57208..58572
                     /gene="mnmE"
                     /locus_tag="ROI21_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDX5109154.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(58721..60880)
                     /locus_tag="ROI21_000168"
     CDS             complement(58721..60880)
                     /locus_tag="ROI21_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HDX5109155.1"
                     /translation="MVDNVTVGSVCAQSPSFMPDLDGGKNKTQLIVDDIVAYLKNPSV
                     YSLEKEGPLNHFVNYCSEVELGFYSDGAYSILVSRSKQQPEGMILTVSDADAINIVHI
                     SVSPALIKFLDDIFTCLHTYPDDESFTKEQIKANSKYDVINYNCKIFFNEKPENEIVC
                     RHLSLQYCIDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQQFDNLQKNPPPGTCVVA
                     CDKFGEALSVFFHRMEKEKLTHMAAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPN
                     ATNTAVRYKANNCDSFGSLRSFMNIKQAQKKWVMTEICSDCVGITPYLPREQAHLLSG
                     INNELQPPLSPLALHLLIQMGMHENIVLFFDKFKNSQEMTASKVLAAKSITGTYGLYV
                     LWYQNSIDVFNEFIINLKELTRKYNFSHSDLENILLAKDYLGVSWIPKALRNNQNKIV
                     KEWLLAIDDFEKEFGVNKNEILRSVGKEIDSIYDLNSAIRTNNYNVVNILLANIKAKM
                     FKNEINKEDILKLMAAREWTGESDKWTKASGLYSAIVKGHTEIVAAWMETAEVIASHY
                     ENDKDVVRELLSLSRNNAACSLHIASFKKMSKEVIDVYLNAAIRLALQHGFSFDEIVE
                     QFTRDFDGKSFSHVVNNGDDIHMGLWLKIFKIVVGENENYLKDVMMQLEEKNNEGKSV
                     ISLANGNPVLKELFWKAVDEFNFPQEELNRLKQYRSL"
     gene            61280..61354
                     /gene="tnaC"
                     /locus_tag="ROI21_000169"
     CDS             61280..61354
                     /gene="tnaC"
                     /locus_tag="ROI21_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDX5109156.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            61575..62990
                     /gene="tnaA"
                     /locus_tag="ROI21_000170"
     CDS             61575..62990
                     /gene="tnaA"
                     /locus_tag="ROI21_000170"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDX5109157.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            63082..64329
                     /gene="tnaB"
                     /locus_tag="ROI21_000171"
     CDS             63082..64329
                     /gene="tnaB"
                     /locus_tag="ROI21_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDX5109158.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            64465..65640
                     /gene="mdtL"
                     /locus_tag="ROI21_000172"
     CDS             64465..65640
                     /gene="mdtL"
                     /locus_tag="ROI21_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDX5109159.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAALAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            65615..66574
                     /gene="yidZ"
                     /locus_tag="ROI21_000173"
     CDS             65615..66574
                     /gene="yidZ"
                     /locus_tag="ROI21_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDX5109160.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            66731..67480
                     /locus_tag="ROI21_000174"
     CDS             66731..67480
                     /locus_tag="ROI21_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDX5109161.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHALDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            67502..68068
                     /gene="yieF"
                     /locus_tag="ROI21_000175"
                     /gene_synonym="chrR"
     CDS             67502..68068
                     /gene="yieF"
                     /locus_tag="ROI21_000175"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDX5109162.1"
                     /translation="MSEKLKVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKS"
     gene            complement(68122..69459)
                     /gene="adeP"
                     /locus_tag="ROI21_000176"
     CDS             complement(68122..69459)
                     /gene="adeP"
                     /locus_tag="ROI21_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDX5109163.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTECVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            69626..70291
                     /gene="yieH"
                     /locus_tag="ROI21_000177"
     CDS             69626..70291
                     /gene="yieH"
                     /locus_tag="ROI21_000177"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDX5109164.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVELEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELAQLPALWKARGWK
                     ITR"
     gene            complement(70428..72836)
                     /locus_tag="ROI21_000178"
     CDS             complement(70428..72836)
                     /locus_tag="ROI21_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HDX5109165.1"
                     /translation="MVAKLKPDVLFNTNPFLEAMNKERLNHKGYCDKIAVSINNKKYN
                     VNSKDIENILDGKGDLFKKRTLWEFVRDLFPGSHIKEVKGLIYEFVTKVDNKAEVFDK
                     IKSLAKKEQQWRFSTKTDFTTNENNEVIVSRSFNLYTGATSNDNEKKQVSSERLTLDN
                     YIDDLHFDNSPLKRFMFDDYAVKLTTLIKNKIPIINTTINLSSLSKDVLNSLKYCSFK
                     NVVFSGVINSPNLEGPVFENCYFEDCKFNNIQLYETNDDTVGSGNKKPIKGMFKGCFI
                     SKCKIENYRCETSKIYTVTQPDSIQEKLGSYLFMQSFVQDCIIQGGCCPGSSILLSHF
                     YNCNIIGLDAHGMDFLANSFNKSNYDEIRGPDTGTVFYNCNLKHIKINDGLREDGSDR
                     SMFNPKEYFSDYIERRSRNLKLDDNFSKCVESIKKGTDSSFSKKNTENNNVFFKNSNL
                     IGANLGYYYSGDRCKDCAIDPNTNYSKDGLTAQKYMYMDLDGAISKEQISDFLKKVSA
                     INIDVINFYNSESKTKEKYKNAFLELESFLSTLYVENKSYEGKYHFDNSRIEFFIFKD
                     MQVKAQNIISNMIENERIKFVESIINKMIPPPDGTILTENPTEFIQKKIKESHKESVR
                     NVTFEYKELAPIFEGVEEKQIKALSNQLEHIKSFKTDYDSRLNKYARDFHYFSSVFAI
                     NREDLLKNYQEIRASIKDYDDLLREIKELTIKRDKSVDDKRTLFDNKRDKWNSQEVQD
                     EVAALNQKIADDDNKIRSNLNIVKNHRRENQYKANKNISNAMLNILDWFERYPDIVKN
                     INQA"
     gene            73142..74329
                     /locus_tag="ROI21_000179"
     CDS             73142..74329
                     /locus_tag="ROI21_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDX5109166.1"
                     /translation="MDNLIGTPPNHAVPHKYMDMEQMEYLCHLNRFSKLSDDFLINGQ
                     REDLFTYKYVLDGSFSNLQHLLPKGQLQKIQQRLSSLMYKNKFHCFHVFLLKLCSIES
                     IPLPNADYAFFDDEMPFTLTDEQIENISFLNAYHKEKKGNNDIVTFDFMSADHHHNFS
                     TTIALTNDSFHISSINNHNAQVIFDENIHLHPYELPESSQWCYQLIKNMISLHCRYNN
                     NFKIKKTCCFDFKNKSFCNARLQYITFIACLFSHANMKYSTFHDVNLDMCEIKNCNFD
                     NSEMNFISCVGTNFSGSTFNNVKTTTAQLIKTPTKWTNNTLKYWFSSCNKRNIIFTFN
                     TISDRNMKLKGIKDILLSLVDQKVNIYSVRQELLDFLNNDLYKNNGEILSYKESIMLF
                     CAV"
     gene            complement(74799..77183)
                     /locus_tag="ROI21_000180"
     CDS             complement(74799..77183)
                     /locus_tag="ROI21_000180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HDX5109167.1"
                     /translation="MSVDDVGKYSVTHKDITNILAGDSQSFLKRSLWEFILDLFPMYH
                     IKEAKQAIIDFVSETGDKADIFNKIKSLADQDQQWRFNATTEFIVDENKDIIVSRVFK
                     ININAQDHHIDAEKKTMHSLFSEKISFDIYKHDIHFDEKAFQSGMLKIQFPNDNEKLS
                     VYLKNKIPFENITIDLSALEKEALASLKYCNFKNVTFTGNISYENLNGSVFENCFFEG
                     CKFENVSLISSRCRVKLNNEIPIYGVFKECFLYRCEIKNFNIDTSKIYSVNQDPNVGK
                     PVGAYLFMQSFVYECTLLNGVCKNASIIASSLLCCKIAKLNGAEMDFIGTSFYHKYRC
                     ELKYDNHFYMCDFSSSDMTCKRNGIRKDLLDFDPREYFKKDGGKLNLDKHISNLIYVN
                     LFPNMSGGRYINDKYVADDIDKYLKLSNCNLLKTCLGESINGIKLDDCAIDPSTTWLS
                     GNLAANSQIRIPVYMDMKGAIAENGISDFMENLMEVNSWVEEFNNYGKDAISHYEKEL
                     LYLQSLLVKLGSFNLDDEGKKYQLNGEAIQYIILNVLYNIEKDIANEIDIDKVNFMHN
                     LYTGFFWQRHYDERNYLKINRSAISNSARFHFHFNFNDLRDIYKDYNNNHEDDNFDKM
                     QKIFLALNEFKERTEINYRDYRKVSKYVDDKVDLFSSIEQYLRWVIEKNNDYNKENYG
                     DIKDKYAKLIVERNDLVDTKDQLIKEGFYLNNKDNKDKKDAERINEIKGIIKVIDGKI
                     PNISKEILHLKDETERLEKEYQQENTLNDVVQNIRSWLKENENMVKAIKKIDTE"
     gene            complement(77150..77297)
                     /locus_tag="ROI21_000181"
                     /pseudo
     CDS             complement(77150..77297)
                     /locus_tag="ROI21_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(77723..78448)
                     /gene="phoU"
                     /locus_tag="ROI21_000182"
     CDS             complement(77723..78448)
                     /gene="phoU"
                     /locus_tag="ROI21_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDX5109168.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            complement(78463..79236)
                     /gene="pstB"
                     /locus_tag="ROI21_000183"
     CDS             complement(78463..79236)
                     /gene="pstB"
                     /locus_tag="ROI21_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDX5109169.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(79327..80217)
                     /gene="pstA"
                     /locus_tag="ROI21_000184"
     CDS             complement(79327..80217)
                     /gene="pstA"
                     /locus_tag="ROI21_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDX5109170.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(80217..81176)
                     /gene="pstC"
                     /locus_tag="ROI21_000185"
     CDS             complement(80217..81176)
                     /gene="pstC"
                     /locus_tag="ROI21_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDX5109171.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(81262..82303)
                     /gene="pstS"
                     /locus_tag="ROI21_000186"
                     /pseudo
     CDS             complement(81262..82303)
                     /gene="pstS"
                     /locus_tag="ROI21_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
     gene            complement(82617..84446)
                     /gene="glmS"
                     /locus_tag="ROI21_000187"
     CDS             complement(82617..84446)
                     /gene="glmS"
                     /locus_tag="ROI21_000187"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDX5109172.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(84608..85978)
                     /gene="glmU"
                     /locus_tag="ROI21_000188"
     CDS             complement(84608..85978)
                     /gene="glmU"
                     /locus_tag="ROI21_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDX5109173.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAALFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(86329..86748)
                     /gene="atpC"
                     /locus_tag="ROI21_000189"
     CDS             complement(86329..86748)
                     /gene="atpC"
                     /locus_tag="ROI21_000189"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDX5109174.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(86769..88151)
                     /gene="atpD"
                     /locus_tag="ROI21_000190"
     CDS             complement(86769..88151)
                     /gene="atpD"
                     /locus_tag="ROI21_000190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDX5109175.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(88178..89041)
                     /gene="atpG"
                     /locus_tag="ROI21_000191"
     CDS             complement(88178..89041)
                     /gene="atpG"
                     /locus_tag="ROI21_000191"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDX5109176.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(89092..90633)
                     /gene="atpA"
                     /locus_tag="ROI21_000192"
     CDS             complement(89092..90633)
                     /gene="atpA"
                     /locus_tag="ROI21_000192"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDX5109177.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(90646..91179)
                     /gene="atpH"
                     /locus_tag="ROI21_000193"
     CDS             complement(90646..91179)
                     /gene="atpH"
                     /locus_tag="ROI21_000193"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDX5109178.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(91194..91664)
                     /gene="atpF"
                     /locus_tag="ROI21_000194"
     CDS             complement(91194..91664)
                     /gene="atpF"
                     /locus_tag="ROI21_000194"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDX5109179.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(91726..91965)
                     /gene="atpE"
                     /locus_tag="ROI21_000195"
     CDS             complement(91726..91965)
                     /gene="atpE"
                     /locus_tag="ROI21_000195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDX5109180.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(92012..92827)
                     /gene="atpB"
                     /locus_tag="ROI21_000196"
     CDS             complement(92012..92827)
                     /gene="atpB"
                     /locus_tag="ROI21_000196"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDX5109181.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(92836..93216)
                     /gene="atpI"
                     /locus_tag="ROI21_000197"
     CDS             complement(92836..93216)
                     /gene="atpI"
                     /locus_tag="ROI21_000197"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDX5109182.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(93833..94456)
                     /gene="rsmG"
                     /locus_tag="ROI21_000198"
     CDS             complement(93833..94456)
                     /gene="rsmG"
                     /locus_tag="ROI21_000198"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDX5109183.1"
                     /translation="MLNKLTSLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLHVPALDGERHLVVIKANKI"
     gene            complement(94520..96409)
                     /gene="mnmG"
                     /locus_tag="ROI21_000199"
     CDS             complement(94520..96409)
                     /gene="mnmG"
                     /locus_tag="ROI21_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDX5109184.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(96788..97231)
                     /gene="mioC"
                     /locus_tag="ROI21_000200"
     CDS             complement(96788..97231)
                     /gene="mioC"
                     /locus_tag="ROI21_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDX5109185.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDAFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(97321..97779)
                     /gene="asnC"
                     /locus_tag="ROI21_000201"
     CDS             complement(97321..97779)
                     /gene="asnC"
                     /locus_tag="ROI21_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDX5109186.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            97931..98923
                     /gene="asnA"
                     /locus_tag="ROI21_000202"
     CDS             97931..98923
                     /gene="asnA"
                     /locus_tag="ROI21_000202"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDX5109187.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(98928..100379)
                     /gene="viaA"
                     /locus_tag="ROI21_000203"
     CDS             complement(98928..100379)
                     /gene="viaA"
                     /locus_tag="ROI21_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDX5109188.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(100373..101869)
                     /gene="ravA"
                     /locus_tag="ROI21_000204"
     CDS             complement(100373..101869)
                     /gene="ravA"
                     /locus_tag="ROI21_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDX5109189.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFNGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            102092..103961
                     /gene="kup"
                     /locus_tag="ROI21_000205"
                     /pseudo
     CDS             102092..103961
                     /gene="kup"
                     /locus_tag="ROI21_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
BASE COUNT        25853 a        26937 c        24941 g        26254 t
ORIGIN      
        1 gcatcggtga ccctcacctg cagcgtgttc gccgtcaaac cgtccgcctc tgaaccatcc
       61 ttaatgacca ccagatcagc gatctgcgcc gtcctgacat cggcaacaaa cgtcacgtcc
      121 cggctcaggc tgctgttgtt gatggatgca gtgacaatac tgatccccgc cgtctggctg
      181 gtgacactga tctccaccgt accgtccggc cctgtgatga cagtcgaagc gaccattgca
      241 ctgttgtctg ccgtcacgct gaccgtctgt ccgccaagcg tattaccgaa tgcatccgtg
      301 accctcatcc gcagcgtgtt cgccattgcg ccgtcagcca ctgagttatc ctggataacg
      361 accagatccg ctatttttgc cgtgctgaca tcggcgacaa acgtcacgtt ccggctcagg
      421 ctgctgctgt tgatgctggc agtgactgta ctggtgcctg ccgtctgact ggtgacactg
      481 atctccaccg tgccatccgg ctttgtggtc accgtcgaag caaccgttgc gctgttgcct
      541 gccaacacgc tgaccgcctg tccggcaagc gtattatcgt tagcatcggt gaccttcacc
      601 tgcagcgtat tcgccgtcga accgtctgcc accgcgttat cccggatgac ctccagttca
      661 gcgatcttcg ccgtcctgac atcggcgata aatgtcacgt cccggctctg actgctgctg
      721 ttgatgctgg ctgtgactgc actgattccc gccgtctgac tggtgacact gatttccacc
      781 gtgccgtccg gctcggtgat caccgtcggg gcaaccgttg cgccgttgcc tgccatcacg
      841 ctgaccgtct gtccggcaag cgcattaccg aacgcatcgg tcaccctcac ccgcaacgta
      901 ttcgccattg caccgtctgc cactgagtcg tccttaatga ccaccaggtc cgcgatctgc
      961 gccgtgctga catcggcgat aaacgtcacg ttccggctct ggctgctgct gttaatgctg
     1021 gcagtgactg tactggtacc cgccgtctgg ctggtgacac tgatctccac cgtaccgtcc
     1081 ggctgagtgg tgaccgtcgg ggcaaccgtt gcgccgttat ccgccaacac gctgaccgtc
     1141 tggccggcaa gcgcattacc gaacgcatcg gtgaccctca cccgcaacgt attcgccgtc
     1201 gaaccgtctg cctctgaacc atccttaata accaccagct ccgcgatctg cgccgtcctg
     1261 acatcggcga taaacgtcac gttccggctc tggctgctgt tgttgatgct gacggtgact
     1321 gcgctaactc ccgccgtctg actggtaaca ctgatttccg ccgtgccgtc cggctttgtg
     1381 gtttccgtca aagcgaccgt tgcaccgttg tctgccaaca cgctgaccgt ctgcccggca
     1441 agcgcattac cgaacgcatc cgtcaccctc gcccgcagcg tgttcgccgt cgaaccgtcc
     1501 gcctctgaac catccttaat gacctccaga tcagcgatct tcgccgtcct gacatcggcg
     1561 ataaatgtca cgttccggct ctggctgctg ttgttgatgc tagcggtgac cacactggtt
     1621 ccggcggtct ggctggtgac tgagatctcc accgtgccgt ccggttgcgt ggtgaccgtc
     1681 gaagcagtcg tggcaccgtt gcctgccaac acgctgaccg tctgtccggc aagcgtatta
     1741 ccgaacgcgt cggtaacctt tacctgcagc atgttcgcca ttgcgccgtc tgccactgcg
     1801 ccgtccttaa tgaccaccag atcagcgatc tgcgccgtcc tgacatccgc gacaaacatc
     1861 acgttccggc tcaggctgct gttgttgatg gttacagtga ctgtactgat ccccgccgtc
     1921 tggctggtgg cagagatctc caccgtgccg tccggccctg tgatgacagt cggagtgacc
     1981 gttgcgccgt tacctgccat cacgctgacc gtctgtccat tcagcgcatt cccgaacgca
     2041 tcggtgacct tcacctgcag cgtattcgcc gtcgcgccgt ctgccactga gccgtcctta
     2101 ataaccacca ggtccgctat cttcgccgtc ctgacatcgg cgacaaacgt cacgttctgg
     2161 ctcaggctgc tgttgttgat gctggcagtg actgtactgg ttccggcggt ctgactggtg
     2221 acactgattt ccaccgtgcc gtccggctga gtggtgaccg tcggggcaac cgttgcgccg
     2281 ttatccgcca acacgctgac cgtctgcccg cccagcgtat taccgaatgc atcggtcacc
     2341 ctcgcccgca gcgtgttcgc cgtcgaaccg tccgcctctg aaccatcctt aataaccacc
     2401 agatccgcga tcttcgccgt cctgacatcg gcgacaaacg tcacgttccg gctctggctg
     2461 ctgctgttga tgctggcagt gacagtactg actcccgccg tctggctggt gacactgatc
     2521 tccaccgtgc cgtccggctc tgtagtgacc gtcggagaca ccgttgcgct gttgtctgcc
     2581 atcacgctga ccgtctgccc ggcaagcgta ttcccgaacg catctgtgac cctcacccgc
     2641 agcgtgttcg ccattgcacc gtctgccact gagttatccc gggtgactac cagatcagcg
     2701 atcttcgccg tcctgacatc ggcgacaaac gtcacgttcc ggctcaggct gctgttgttg
     2761 atactggcgg tgatcgcact ggttccggcg gtctggctgg tgactgagat ctccaccgtg
     2821 ccgtccggcc ctgtgatgac cgtcggagca accgttgcgc tgttgtccgc cgtcacgcta
     2881 accgtctggc cggcaagtac attcccgaac acatcggtca ccctcacccg cagcatgttc
     2941 gccgtcgaac catcagccac tgagccgtcc tgcgtcacca ccagctccgc gacctgcgcc
     3001 gtgctaacat cagcaacaaa gttcaccgtt ttactatcgc tgctttgatt gaccttagcc
     3061 tcaacagtgc tggcatccgc tacggtactg ctcagatcaa cctctgccat accatgcgca
     3121 tccgtcctga tatctgcgct ctgccctgca ttggcaaata cagccgcccc cttcgtcacc
     3181 gtaaaggtga cggtctgctc cggcagcaca ttgccaaact gatcttccac ccggacattc
     3241 actgtatgtc gatcgctgcc attcgccagc acatccgggt tacccgttgt cacttgcatc
     3301 gcgacaattt gtgcgctggc ggcatcgcca ctaatactgt aatcatttga agtcaaagcg
     3361 ctcgcccagc cggacatctg tagctgtgct tttaaaccgg tgccagggag taatgcggta
     3421 tacgttgcgg tataaattcc tttttgatct tcattccagg cgatgctttc cgcttttgta
     3481 tttggcagct gaatagcctg gttgatagcg tctcgttgcc ccccgaccag gttttcatag
     3541 ctatccttga gtgttaccgt aacttttatc gcaccgcccg cggtgtaggt cgttttgtcg
     3601 gtaacgatgg aggaataagc cagcacagct ccgctttcgc ggatggcata cgcatcggat
     3661 tgctgcgcgc tactccatgt cgatagtttc agcgtggcgt aatggcgatc gccagcaatc
     3721 ttcgcggttc gggtggtggt gtaaacgccg tcagactctt cgctccagtt acccatttca
     3781 tttttatctg taccgctcac ctcgaccaca ccgctacccg ccagcagcga agccatccct
     3841 ttcaccaggt ttttctgctc atccataagg gtaactgaca cttttatctt gccacccgca
     3901 gtgtatgaca catcgtccgt cttgatggtg gaggtcagct gcgagatatc gccagtaata
     3961 aagctcactt ccacagactg actactgcca ttgatctcag ccttgataag actgtttacc
     4021 gcctggtcac tggcaaggtt aacgctggca atcccgtctt tatctgtcgt cacaataccg
     4081 ccatttggat cggcaaaaac cgccgcacca ttcacgaccg tgaaggtaac attttgtccc
     4141 tgcagcaaat tttgatgcac atccctgacc gtcacagcga cggtattgtg atcgcgtctg
     4201 gcaactgcgt tattcgcggt gatctgcatc gcagcgattt tggcggtatc tttatcaccc
     4261 gcaatactga acgcatccga atgtttggtt tgccagccgg gcatcgtcag ctgggccgtt
     4321 aatttgtcgc caatgagatg ggccgtatag cttgctttgt aaattccctc actttcttcc
     4381 gtccaggcac tcacggcatc agccttcgtg ttgtcaactt taacggtttg tttgagaagt
     4441 tcttttcgtc cggtaacccc gttgccgtta tcatctctca gggtcacttt aacgactatc
     4501 ggcttacccg cgacgtaatt atcctggtct gtctcaatcg tggagtcagc gcgggatgct
     4561 gtattcgcca cgatagcaat cagcgctggc gtcttagcga tatcatcgcc gttaaattgc
     4621 ggcatcagcg ataacgcgcc agacgtcttc ccggcggtaa ctatttgggt ataggttccg
     4681 ttaccgttgt ctttccattc gcttaaggcg aaatcctgca aacctttcgc ctgtgttttt
     4741 aacgtcatac cagggattgg cttgccgctg gaatctcgcg cggtataggt cagcattgag
     4801 gttgattttc catcagcaag caggatttgc agatcgacag aaagcgttga atcggcgagg
     4861 cttaatgtgg gtttttccac gaccaccata ctttcttcac gccgggagaa atttcctttc
     4921 gaatcttccg ctgtaaccgc tatcgggtaa gtgttgtcgg tttcaggcat tgccgtgaat
     4981 cggtagggag gaatggtgac ctgaatatcc ttgccggaaa cagctacttt tccgtccgct
     5041 gattgcaggg aggcggcttc aatgtcataa cctttcagcg catatttggt ttgtaaggaa
     5101 gaaaccagcg atttaacctc acccggcttg cctttcaacg gatcggtcag cgtcagccgc
     5161 accagctctt tcttgcgata ttcaaggaca atattgttat tacgatcgac caggtcatag
     5221 cgactgccca caaggctgcg tcgtgctgca acttcagccg gatcgagctg tttttgcagt
     5281 gaatgaccgg gttgcaacgt caactccata ccaattcggg tgtcgttttc tccctgtttg
     5341 ccctgccgtt gctcggcact aaaggagatc agaggaacag gggtataact caaacctgcg
     5401 gtaatcgcgt gaggatcatt ttgtcgctcg tctttaccaa acagcgccac ttcatcgcca
     5461 taatattgtt catagaccaa cttgccgccc agctgcggcc aggccggtaa ccagccttcg
     5521 gcgcgaagat cccacccgtt cgccggacgt gcttcatagt cattgtcgag ttccggtgca
     5581 ctacgccagt tactgaggcg cagataaccg ttgccgctca gtttcaggta atcgcgccag
     5641 tattcaacgc ccatcccggt tcgggtgtga tagcgggtca ggtcatgatc gataaacata
     5701 ttgacgccag acatccagct cgatgtgaaa taacgccagc cgataccgtg gttggtttga
     5761 gtgcgatcat ccgtacggtg aagtgtgtgc tggcttaaga ccaggttatc cggcgtttcg
     5821 taccagggat ggagaaattc aaatgacgag cttttaaggc tgaaatcttc atccacacca
     5881 agagataccc gggcagttcc ccaacgactg agccagtccg ttgcctgcgc agatgcagaa
     5941 gaagccaccc agccacgcgc catactcgcc gcctgttcgc tgtccatatc gcgtgctaaa
     6001 agtgttccgc tttgctgcgc cacttccgcg acctgcattt gcggatcttt gccatcgcgc
     6061 tccatctgca ttgctttcag gttgcgcgct tcagggctgt tactgttaat gaggggcaca
     6121 tctatttcat cgccctgacg cacatggtca aagccccgcg caaaggtacg gaactgattc
     6181 aggcgacgta attcatcaac ggtgatacca aagcgtgccg cgaccgttgg tggcgattcc
     6241 aacgcaccca gggtatatgg aatagtttta acgttcggca ggatcagtga cgcggcagta
     6301 tccgagtgcg caggattaat atgcgtcggg gcagcagcca taacagggaa aatagtttgt
     6361 gttatcaggc aaatacctgc ggtcagacga cgtaaacgat cccccaatcc acatatggtg
     6421 ttatcgcccc gacgatcgcc attcccatgc gctttccctg ccatttgtca tccctgtcgc
     6481 tatgcgttca gattaaatga cctttcttta acatgagaga aaatgatgat tcatgattag
     6541 caaatgatga atacaggcgg aataatggaa ataacaacaa gataagaaca atacctgatc
     6601 aagggttatc agggggaaga acaggagagc tttcccgcca gagcaataac cctcctgtaa
     6661 atacgttact tcattttcac ctttgcggtg accaataacg ccgtcagcaa catcaatgtg
     6721 ccggacaaca tcaacggcga agtcagacca atattatcca gcgcataacc gccgattgcc
     6781 gcgccacagg tattagcaag ctgaataacc gccacctgaa tagacccggc tttttctgcc
     6841 tgatcggcca gtgagcgagt gatccacgtt gaccagccga caggaaccaa tgcaaaagtt
     6901 agcccccaga taatcgccac gccggtagca acgattttat cgcttcccca caacgtcagc
     6961 accaacgcac tcacagccag tattaacggc gcgcctgcta aggccagttt taccgaacgt
     7021 ttaagaatga acgacgaaag cgacgtacca ataaagctgg cgataccaaa actcaacagc
     7081 accagcgtta agccatccac gccgaatccc gccaggttca tatacaccgg gcgaatatac
     7141 gtgaagaaag caaactgccc ggcgaaagac atgaagatgg cgatcatccc tgccatcaca
     7201 cccggacgtt gtaataagcg gaaagtattt tgtttctgat gcgagggttc gcctggcagc
     7261 gaaggcaatg atttgataat ccagaaaata cacagcacgc ccatcaccgc cgccgcatta
     7321 aagacattgc gccaaccgat aagctcgcct aaaaaactgc ccaacggtgc ggcaatcacc
     7381 agcgcaatag aaaccgcgcc gaagatcacc gacagcgcct tcggcaccgt acgcggcggt
     7441 accagacgca tggtcagcga cgccgacatc gcccagaacc cgcccagcgc cagccccaga
     7501 caggcacgac cgattaaaag caaactgaat gagttagcaa aggaaaccag caagcaggag
     7561 agcgtcagca aaacggcaaa caaaataaca acgtagcggc ggtcagtggc ctgaattgtc
     7621 tgggtaataa acaaactggc aaacattgcc acaaaggcgg tcacggtcac cgattgcccg
     7681 gcaacccctt ccgaaatgcc taaatcctgg gccattggcg tcaacaaact gacgggcaaa
     7741 aactcaacga taatcagaca ggcgacgcaa aacgccaccg agaatacggc tgaccagttc
     7801 ggtcgggtga tgacatccgc gccgcggttt tcggcaataa attcactcat ggtgttaccc
     7861 gtggcatgtt tcaggggaaa agccgaccac tttaacattg aaatgtgtga cgcatttaac
     7921 gtttttgcaa cttttacggt gttgtgggaa aagttcgagg ctgctcgaag aaataaaaat
     7981 catagggcaa ggtgatatgt attttcgcga ttgtgttggg aacaagggat aaagtattgc
     8041 acttggtgat atactttaat aacaggacct caacgatata cgcgtgttta aaaccaaatg
     8101 gtttgcaagg gaagccaggt ctcatgcaat aacagatgaa gagttgtgcc gtgccatttt
     8161 agaaacggaa caaggtaaag ctgatgcact tggcggtggc gtttttaaaa agcgccttca
     8221 ccaaaatcgt gagcgtgcaa ttattctggc aaaaggggga agcaattggt tttacacgtt
     8281 tttatatgca aaacaggata tgtcgaacat caatagtcag gaactggctg ggtttcgtga
     8341 acttgcaaag cattatgcct tcctgacgaa agcccaacta acagcaatga ttaacactaa
     8401 agaactgaca gagatctgct atgactgcaa aaactaaatt taaaagtcct gcatttgagg
     8461 ctattcacag cgcagcggct ggattgtcca gcgtcgatgc tatttctgct gaaacgatgc
     8521 gtacttttga caaagcctgc ctgaccagcg tgcaagactt acaacctgtt gagataaaag
     8581 cactgcggga aaaattaaaa gtgagtcagc ccgtcttcgc tcactatctc aataccagcg
     8641 tttcaactgt acaaaaatgg gaaagtggcg ctaaacgccc caatggcatt tcattaaaac
     8701 tgctcagcat tgtgcaaaaa cacgggctgg aggtgctgct gtaaatagaa cgtagataat
     8761 gcgttcacct actgtcagtt ccgataccgg atgcggcgtg aacgccttat ccaccctaca
     8821 ggtcaggcgc ggtctgtagg ctgataagac gaagcggttt taatgcagcc agtgaacgcc
     8881 ctcggcaggc tgaaagaacg cgttatagcg catctgaaaa gcattaatat cctgcatttc
     8941 aggttcggtt tcgcgcagaa aacccagcgc cagcctgacc tgagcatcgg taatcggcgc
     9001 ggcaaggcgg gctttttgta aaatccgatg ccattgcagc aggttctgtt cgctaccatc
     9061 gacaatgaca atttgccaga tgagcaatac ctgcgcggcg ttttgcaggt gcgactcatc
     9121 cgggcgatgc aaataacgta aaaattcgtc accggctgac tttcccattt gatcgaccat
     9181 cgactccacg ggtacgacat cgatattcag ccgatcgctg agacggcgaa tcgcttttct
     9241 ggccccggat gtcagcaccc gaaaaccaac cacaaacact accgccagcg tggcgagcat
     9301 tatccagatc atgagttctc ctgcgtaatc agaactcatg atatctggaa accctcgcca
     9361 gttactaatc caccagaata atcttcagtg caaacagagc tgcgaccacc accacacaca
     9421 ggttcagatc gcgccagcgc ccggtgcaca ctttcatgat gcagtacgac ataaagccga
     9481 gtgcaatccc ttcggtgatc gagaaagtaa agggcatcat caccgtggta ataaatgcgg
     9541 gcaccgattc ggtaaaatca tcccagttaa cgcgcgccag gctcgaagtc atcagcacgc
     9601 caacaaagat taacgctccg gcggttgcgt aaggaggaac catcgccacc agcggtgaga
     9661 agaacataac caacaggaac ataacgccga ccacaaccgc agtcagcccc gtgcgaccac
     9721 cgactgccac accagaagta ctttcaatat aggcggtaac agacgaggtg ccgataaacg
     9781 cgcccgccac cgaactgacg ctatcaacat atagcgcctt attcatattg gggaattgac
     9841 cgttactatc tattaagcca gctttatcag ttacaccaat taatgttcct gacgaatcaa
     9901 ataggttgat cagcataaag gagaaaatga taccggcgag ttcaagtgtt aacgcgccgc
     9961 tcaaatctac ttcaccaatg acgccgctaa tatcaggcgg aatggaatag acgccgctaa
    10021 aatgaacatc accgaaaaat aagccacagc aagacgtcac cacaatagaa accagcaccg
    10081 cggcatgaaa atgacgtgat gacaacacgg ttataataaa aaaccctaaa atacctaaca
    10141 acacgccgtg agaacttaaa tcgccaatca tcaccagggt gtctttattg gcgacaataa
    10201 cgccagtgtt ttttaatccc attaaggcaa taaataatcc gattccgctg gtgataccaa
    10261 tacgtaaact taatggaatg ttggagatca tccagtaacg gatacgaaac agcgtgagca
    10321 aaaatagccc aattgcgccc cagaatatcg cgcccatccc ggtctgccag gagataccca
    10381 tcgcccccac gaccacgaac gcaaagaagg cgttcagccc cattgccgga gccagcgcca
    10441 cgggtaagtt agcaaatatc cccatcgcaa tactgccgat accggcaatc aaacaggtgg
    10501 taacaaacac cactttcggg tccatttgtg ccgcgccgag gatttgcggg ttcacaaaaa
    10561 cgatgtacac catggttaaa aaagtggtca tccccgcaat caattctgtg cggacggtgg
    10621 tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat
    10681 cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta
    10741 aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat
    10801 attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca
    10861 aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta gccgggcgga
    10921 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
    10981 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
    11041 acgttacatt gctggtgttg gcgcagaata cactgatgct ccggctttgc agcggattga
    11101 tgcccgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag
    11161 catgatgacg ccggtcacct ttgaaaccgc taccctgccg cgcggcctga caactgttat
    11221 ttgcgaccct catgaaatcg tcaacgtgat gggcgaagcc ggattcgcct ggtttgcccg
    11281 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct
    11341 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
    11401 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
    11461 gaatgcactg ctcgataaac tggatgcatt tcgccacctg acactggacg gtcactgccc
    11521 gggtttgggt ggtaaagcac ttaacgccta tattgctgcg ggtattgaaa actgccacga
    11581 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
    11641 cgaagggtcc gctgcccgca atctcaacgc actggcaccg ttgatcaacg aatttaacag
    11701 cccgcaatgc atgctctgta ctgatgaccg taacccgtgg gagatcgccc atgaaggaca
    11761 catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
    11821 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
    11881 acccggtaag caggctgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
    11941 ggtactggtg aaaggcgaac cgattgacgc gcaaacctta caggcggaag agtcggcgag
    12001 actggcacaa tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag
    12061 cgactttgcc ctgcaattca cgcccggaaa acgctatcgg gtaattgacg tcatccataa
    12121 cgaattgatt acgcactccc gctccagcgt ctacagcgaa aatggttttg atcgcgatga
    12181 tgtgtgcttt attgccgtcc ttgagcgtta cgggcaacgt ctggctccgg cttgtggttt
    12241 gctcggcggc tttggtctga atgaaggcgc gctggctgcg acggtcagcc atgacagcca
    12301 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
    12361 tcaggatggc ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt
    12421 acccattgcc gggctgatga gcaccgacac ggcgcagtcg ctggcggagc aaattgacgc
    12481 cttgaaagcc gccgcccgtg aatgtggtcc gttaccggat gagccgttta ttcagatggc
    12541 gtttctttct ctgccagtga tccccgcgct gaaactaacc agtcaggggc tgtttgatgg
    12601 cgagaagttt gccttcacca cgctggaagt cacggaataa taaaaaaaag cccggcgtca
    12661 tgccgggcaa aagtcaccag ttacgtttat gccactgtca actgctgaat ttttttctcg
    12721 cggcggattt tgcgttcttc catcaccgcc actatcgcca tcaggcagat acaaccaatc
    12781 gcggcgatat ccagcgcagc gaaggtgcct gcccagccgg taaggccgaa taccggcgtt
    12841 ccatcggcaa tcattcccag acctaacttg gcaaagctgt caccaatcag gtaagcgaag
    12901 gtgcctttaa taccatcggc agcgccaatc gcttttttag gtacaaagcc aacagcagcc
    12961 acaccaatca acaattgcgg gccaaagacc aggaaaccca acgcaaagag agaagccagg
    13021 tagatatatt cgttgctggc atgttgatac acaccgagcg tggcgataat cagcgccagc
    13081 gcgatgcagg ccaccaggcc acggcgaccg ttcgccaggt cagagagcca gccccacagc
    13141 agcgtaccga ccagcgcacc agcttcaaac agcgtaaagc cctgaatcgc caccgcttta
    13201 gagagtttca gttcctggaa cgcgtacacg gttgaccact ggtcgatacc aatacgcacc
    13261 acatagagga aaatgttggc gaagcacagt agccagatca ctttgttttt cagcacatac
    13321 tcaacaaaga tctgccactt ggtcatatcg gtagattctg tctctttgtc ctcttcgctg
    13381 atctcctcgc cgaacagctc ttcagctttg ccgaggccat aagattccgg ggagtcgctg
    13441 ccgtaacgca ggccgataaa accgacaatc agcgcgataa tcgacgggaa aataaacatg
    13501 ccgatgacat gaccatcgaa caggtaattt gcgccgaaca gcgccacacc cgctgcgcct
    13561 gcaccgccaa ggttgtgaga aatgttccag aaaccgagga atgtcccgcg tttacgacgc
    13621 ggcgtccatt tcgtgatggt ggagtaactg cacgaaccgc cggtactctg gaaaaagccg
    13681 cttaaggcgt agaaggcaat catcaggaac aggctaaccg agccgctgcc catactggcg
    13741 ctgaagccca gcatacaaat ggcagacagg atcagcatga acggcaggaa ttgtttggtg
    13801 tttttgccgt cggcgtagta ggaaaccagc gttttaccca cgccataagt gatggagaaa
    13861 cccaggccga tcatccccag ctgcgtcatg ctcaacccgt aggtcgagat catgtcgttc
    13921 tgcgcgatgt taaagttctt gcgaatcagg tacatcgtca ggtagccgat aaagaccacc
    13981 aggtaggatt gcatgaacgg tttgaaccac attttgcgcc gcacttcgag cggaaggtcc
    14041 agggtcggct tgcgaacctg gtttaagaaa gccagcatgg gttactcctg atttcccttc
    14101 atacttcaac ccgcaggtgc cttagctgca cgcattcctc ccggtcacat agttcactat
    14161 gctcccggga ctcacgcgtt tgctgcgttc ctgcaacttg aattattttg ggaaagaatt
    14221 aatgaatgat ggcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa
    14281 aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct
    14341 tctcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggca
    14401 atggcgataa ctacaaaaaa tccgctccag tgccaggtat cgagcacttt cgccagcggc
    14461 caaccagcaa gcgacgcccc cagataagca aacaagccga caaatcccgt cgccgcccct
    14521 gccgcctctt tgtgggaaca ttctgccgcc gccatgccga ttaacatctg cgggccaaag
    14581 acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc
    14641 agccacagcg agccgactga aagcaaaatt ccggcagcga aaatcaaatt catcggcccg
    14701 cggttgccgt taaacagttt gtccgagccc caacccgcta ccagcgcacc gataaatccg
    14761 cccagttcaa acattgtcac tgccgtattc gccgtgacca aatcgacgcc cagcgtctcg
    14821 gacatataca aattgcccca gtcgttgatt gccgcccgga ccacatagac cagcacatag
    14881 caaaacgaaa gcagccagat atacggattc agcaacacat acttggtgag gatctcttta
    14941 cgcgtcagcc ctgccccttc ttgttgttga gcaatctcca gcgcgtcgtg tcgccattca
    15001 ccgaccgctg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatc
    15061 cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgcagc
    15121 gcagctgctg ccatcacaat gggaatgagc gcaccgccaa cgttatgcgc cgtgttccat
    15181 aatgcccacc aaccgccgcg ctcggtacga gaataccagg ccgttaacag acgcgcacac
    15241 accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagcacggc aaacgcccat
    15301 agcgacgtcg agaagccaaa cagaatgttg atgatgcccg tggcgataag ccctatcccc
    15361 ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgccg
    15421 taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccatta
    15481 gcaaggattt ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa
    15541 cccagccaga tggtcagcag aatatgccga cgccagtaac gatagcgggc atcaatttca
    15601 tgtttatcag tcattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa
    15661 ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg
    15721 gtcaaggtgc cgcccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt
    15781 tgcccgcaac ccggcggtaa accgctgcca tcgtcttcaa tcaccagcat caaccgttca
    15841 tcctgctgcc agccttgcag ggtgaccgcg ctggcatcgg catgtttcac aatgttgttc
    15901 agcccttcct ggcagacgcg aaacaacgtc actcgctggt tttcacttaa cgctgattca
    15961 tcgattcgcc attcgagatg gctgacaatg ccgcgccctt ccagctccat ttcccgcatc
    16021 agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgacct
    16081 aacaaacggc gcaccgcatc gtaaacgccc agcgacagtt gttcgatgag ctgcccgctc
    16141 tgcttcacac tggcgttatc tgccgcaagt cgctggacaa tgcccgcttg agtacgaata
    16201 gcagtgatgg tctgaccgat atcatcgtgc agctcacgcg ccacgtcgcg gcgcacgctc
    16261 tcttcagttt ctaacaaacg ttcagcgaga tgctgattgc gcgccagttc tttttgcagc
    16321 gactggttaa gttcacgcaa ccgctggata cctgcgccca gtaacaaccc tgtcagactt
    16381 tgcaccagca gtgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc
    16441 agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccagccgta gtgccaggcc
    16501 agcgcgataa tcggcagcgc cagacagaat ggcgtaaagc gcgacagttc gtccggcaat
    16561 cccaactgga gccagagact gataacaaac agcagcaagt accagaccag atgccgcccg
    16621 cgccagttga tcggttgaga aactaacgac ggcccgagcg gcagccagat gttattggcg
    16681 agatagtgcc agaacaccag acatatcggg gccagcgtca agccgccagt taaagtcagc
    16741 aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc
    16801 gccgccgtca gcgccgcacc ctgtaacagc aaggtgcgcc agtcacgttg atggcgatag
    16861 cgcgagatca gcgccacggg caataacgtc agtaaactac cgatcatcaa taaggagaaa
    16921 tgggttaaac cgaccgcctg cgttagccag taaatcagca gccattccgc gcccagcaat
    16981 acgggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat
    17041 aacagcaccg ccatatcagg gcgctcaacc aggtgcaggc tgatactcca caggcaaaac
    17101 catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc
    17161 ttcatcacca gccatcaaac atgcggcgcg ccagttcaac gtcgttactg acgcccagtt
    17221 tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg
    17281 caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg
    17341 ttagcgggtc ctggcgaccg gatgccagtt taatggcaat atccggcgtc agataacagc
    17401 cgcccgtggc aaccgtatgc accgcagcaa tcagttcatc cgggctacag cgtttggaga
    17461 gaaagccgcg cgcccccgcg ttaagcgcct gctcaaccag cgccggactg tcatgaacag
    17521 agagcataat cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat
    17581 caggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcaac cccgccagcg
    17641 cctcgcgccc cgaaccaaac tcggcaacta cttgcaaatc aggttccagc cccagcaact
    17701 gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcataattg
    17761 ggtcctggcg gataaaaaac gcgcatacct taacgataag cgcgatgttg ttcaagttgg
    17821 atatggggta tgtattggct taatgatgtt gggcttatcg tcaaaacgct tacctgaaag
    17881 gtaagcgttt ggtacgtatt attgagaaaa agcgaattat ttgtaggttg cgattgcaat
    17941 attgcctgaa aaatttctcc tcctgttgcg gaaatcgttt tcaggtaaca aagagtcatc
    18001 cagaaaccta taatcttcag cggaacgctc attaagagag tcatccactg agtcgttatg
    18061 aatataatcc tcaccgtatg ttcgataaat gaaattagca atattaaaat cgttaccagc
    18121 ttcatttatt tcggctttta caatataatg agtttgtgta aatgtatgca gtgaaaagtc
    18181 agcaaaaaga aaatttgttt ttcccatcaa agcacaaaac cctgggttac tattaaaccc
    18241 ggcagaaata gaattgcttg cctgtattgc attaatatcc cccctatcaa gcgcgacagg
    18301 aaatgcctgt gaacaagcat aatacaggtt acctattcct gaaatactgg agaggcaaga
    18361 aataatattt tctctggttt ttttctcaac accatctttc tgtagagagt ttctcacaat
    18421 attacattca tctggcgtaa atacaatttc actaaaaaat ttttcgaaat ctttatattt
    18481 tctaacacct ccatgaccag acaaagccat ggttttactc ctcagtaatt catggatagt
    18541 gctatttatt ttaactccac aacatgcaat aaatgagcta gcctctccaa catgtcttga
    18601 tgcatcagca agaagtgcac taaactctcc ctcccgaaca tctagttttt ttatgtctga
    18661 tttataacta caatcatctg gaattaattt atctggaatc aattcatatt ggattgattt
    18721 atctggagtt gattgagttt gttcatataa cgcttttttt aattggtgct cagcatcagt
    18781 aactccactg ctgagttcat ttttaaaatt attattaaca acttctaact taagagcatt
    18841 tgcagcaact gttacttttg agagttgata cttttccaat acatcgtgct ctgataataa
    18901 ccctataaac tctttcacat ttcgaccact aaaatcccta tccctactct ttttttccaa
    18961 actcttgcca tttaactcac gatacacatc agtgacttta aatttatcac tgccggtctg
    19021 ctcaattttt atatagctat ccatgcctgt ggtagcgatc catgttttat aattcgcatt
    19081 cgctgattca ggcgtaacat gaaaataaga aaccaattta ccactgatat catgccgata
    19141 gatatgaaaa gaatctaaaa tgtataataa tatatttcgg aaaacttcac gaaaagtggc
    19201 atctttagat aatgttctac aattatcgag caggccgctt tgctccatcg catcaaatgc
    19261 agcaatatta ccctcgatac tatctcttag actctggcta agaccctgac tctgactctg
    19321 aaactggcta ttatcattct ctgtttccgt tggatatgaa taactcgtca cactaaccat
    19381 agtaacctcg caatatgaca ctttgcatat tttgacattg ttagaagtat caatggctag
    19441 catgataaat atctcaatgc taaccgctat atttcattta tttcaataat aagactatag
    19501 tgccgttaat ttttatcgat ttccccaatg caattgcatc actaacacca cttattcact
    19561 atcaaaaatc gctcattttt tttcaaaaag actgtttaca cacagacggt taacaatacg
    19621 cgttcacctt aatgataaac gcgatttatc aggcaaaaat cataaatcag cagttgttgc
    19681 ggcaccgaaa aatgtaaaag tgactttatc gattttggag ttttgttcgt cgaccctacc
    19741 ggtagtaaaa taacgagaac actgaagctt aacttcattt ccaacattat taaccgccat
    19801 cgaatacatc ttctgactaa acccatttat agtaaaatcc atttcattaa gatactttcc
    19861 agacccaaaa aaagcatttc ctgatttttt taaatgcaat gttaatgaag tggcaccatc
    19921 caaactcttt gagagtaact cgcgaacatc ttgagatgta tgttgtaagc gagtctgata
    19981 gccttgtgcg gaagaataat aaaaacatgt aatcccctga ggactggcta agcaagcgat
    20041 tatttcatca atcttatcat ccgtaatatt accgtttgat tgttttaata attctcttac
    20101 ctcagcacaa tcattaacat caaagcaaag ctgtttaacc atttcttcaa gtgtgttttg
    20161 aatatatttt ggatcttgtt caattttttc aattttgttt gcaaactttt taagaatatc
    20221 tttacttaac tcaacaccac agcaagttat ttttgcagca ccacttggtt tagtttgttc
    20281 ttttatacat ctatgagcat ccatagtcat agagtgtaaa taatcctcag taatatcaat
    20341 tttttcctga acaggtaaat ggccctcatc tttttgaatt cgcaggttta ataaatcata
    20401 tgtattactc acaatgtctg caggcatttc gtaactacaa tccgtattca tcataacaat
    20461 actattaact aattttttcc tcaaaaccgt gtcagcagaa tgtgtttcta acgcagcagt
    20521 tttttcagac agcgatttga taatagtcgc cacagccact ccagaatatg atgtttcttt
    20581 tactattttt tttgtatttt tttcacctgc atcaaactgt ataactttaa aggatttgcc
    20641 acctgtttgt tcgattacgg tatattcgtt ctctccagtt gccgccatcc aggtacgatt
    20701 accattattt tcagtgcctg gcatagggtg aaaatagtta aggatttcgg tattaacact
    20761 gcgagaaccg gacataatat ttgctattaa gcgttttatt tcctgtaaca tccattttgc
    20821 gaaattactg ccaggtgaat attttttctc gttatttgtg atattaagct cagcagcaat
    20881 aattctattt ttatccaaaa aggtggaggg gcagggattt gacatcattg ttccgctgtt
    20941 tatagggctt gccataatat acctcacgat gaaccattaa ttaatagaat gctggtcatg
    21001 accactggta tagacagcaa tcagaattta acaccatcgc tggaaatttt tagccctaac
    21061 tgtcaacccc ctggttatcg gtgcagcgac ttaaattaac gtcctgggct aataaattgt
    21121 gcctgagatg gtctctggca caatttcacc ctatatatct gaacggatct atcaaaaatc
    21181 gctcatcagt aacccccccg agcggtttaa tattgctcct tactgaaaaa acaccgcaat
    21241 cttgttaaac attgtcggat cggactgatt acgctgcact ttcaccacat cttccagctt
    21301 atcgatttgg cttatcatct gctccagacg ctggtcgtca ttgaccagta gccagatatg
    21361 gcttttgtcg ctgtcctgaa tcggcaggca gagaatgcct tcgacgttaa aagcgcgacg
    21421 ggcaaaaagg ccacaaacgt gggtcattac gcccggatgg ttgcgaacgg tgagctccag
    21481 aattacgttg tcatgagttg tgttttgcat ggcttattcc cccaccattt cagtattcgc
    21541 cgcacctggc ggcaccatcg ggtacacttt ttcttcggca tcaatgcgca catggatcag
    21601 cgccggacca gggcgattga tgatttcctg caatgcagcc tgcggatcgg cttcgttatt
    21661 caaatcacag gtttcgaggc cgaatccggc ggcaatctgc ataaagttga tttttcccgg
    21721 ataggtagcg gcaaaaacgc cctgcttgta gaacagactc tgttgctgat gtaccagccc
    21781 cagcgcttcg ttgttcatca gaatgatttt gacatccagc tgattttcac tggcggtcgc
    21841 catctcctga atattcatca tcaggctgcc gtcgccggag aagcacaaca ctttgcgatc
    21901 cgggttcgcc agcgccgcgc caatcgccgc aggcagacca aagcccatcg tgcccagccc
    21961 accggaggtc agccactggc gtgggcgatt gagcggataa gcttgcgcgg tccacatctg
    22021 atgctgaccc acatcggtgg tgataatcgc attgtcatcg acacaggcgg caacggcgtt
    22081 gatcaggccg taatggctta atggatcgca cgctttcggg attggacacg ggaattcgcg
    22141 ctgcaaatcc gctaccaact ggtgccactc tgcacgcggt tgcgcttcca cctgcgggat
    22201 cagctgcgcc agcacgtcat caacatccgc ctgaatcgct acgtgcggct gcttgatttt
    22261 acccagctct gcgcggtcga tatcgacatg aataattttg gcattcgggc agaactgctc
    22321 ggttttgcca atcgcccggt catcaaaacg cgcgccgaga acaatcagca gatccgcctc
    22381 ctgcaagata tagttggtgc tgcgcacgcc gtgcattccc agcataccca gcgacaacgg
    22441 atgcgctttt ggcaacatgc ccagcgccat taaagtcatg gtggtaggca gctgcgcttt
    22501 ctccgccagt tcacgcactc gcgcaggcgc attgatcaca ccaccgccca gataaagcac
    22561 cgggcgtttg gcagcattaa tcattgccgc tgcgtcacga atgctttctt cgctaaaggc
    22621 gggcgcgacg gctttttctg ccaccgcggg ctgtgcttca atctcaaaaa ctgccgtttg
    22681 cacatcctta ggaatgtcta tccacaccgg gccagggcgg cctgattgcg caatacggaa
    22741 ggcatcgctc atgacctgcg ggagttcttc gatatgtctg accagatagt tgtgtttggt
    22801 gatggggata gagatgccgt aggtgtcgac ttcctggaag gcgtcggagc caatcatcga
    22861 ggcaggaacc tgaccggtga tgcaaatcaa cgggatggag tccagccgcg catcggcaat
    22921 ggcggtcacc agattagtcg cacccggtcc gctacaggcc atacagaccg ccggtttacc
    22981 gtcggtgcgc gccattccct gagcgataaa ccccgcgccc tgttcatggc gagccagaat
    23041 atgacggatt tgcgtgcttt ggcttaaggc atcgtaaaca ggcaggatag aaccgcccgg
    23101 aatgcccgtc acaatcttaa tgccctgctg ttccaggaaa tgaacgataa attctgcgcc
    23161 ggtaaagcgc ttacgcgttg atgttgtgcc cgaacttgcc atgctccagt ccttttcttc
    23221 tgggccgact ttccgggagg tgcttaaacg aaaaaccccg cccggtttgc gccggcgggg
    23281 ttttggaatc gtgtgttgtt ccagtcccta cggcgcattg ccgacgacca ccaccacacg
    23341 cacgacgacc actgcggcgg atggcgcagt tggtagtagt tttgcgttga gcatggaagt
    23401 agtcattggg gaccttgttg gttttgtgtt taacaatatt tatacaagca cagctttaca
    23461 ggggagacaa tggaaaattt ttcagcaagg gaaaattgag gggttgatca cgttttgtac
    23521 tgtactgcag gtaacaaaaa accccgccgg agcgaggttt cgtcagccgc ccgcggctgg
    23581 taaccgcaaa gcacactgta ttatgtcaac acagaaagta tacgtgttcc gcgcaaaacg
    23641 cgcaatttcg gcacgaattt tgacgtattt agtgcatagt tgagtatcga tcacagtttg
    23701 cgttttgtcc aaatattact gtttatttat acagcaaact tctataatat cactgtacgc
    23761 aatgtgttat gcgggggccg catcgttacc cggcgcacta agtcctggct gaaacgggtg
    23821 gtgccgtcag cgccttaacc ccgcgtgagc acactgtgtt atgtcaacga gcacaacgtt
    23881 tctccttgag ataccgcgtg cacaacagct ggcaacaggc agcggaaagg tacgtcagct
    23941 ggcagtgctc ctgaaccaca ggagacgcgt atgagcctgg tggatatcgc cattcttatc
    24001 ctcaaactca ttgttgcagc actgcaactg cttgatgctg ttctgaaata cctgaagtaa
    24061 ttcagattca agtcgcacca aaggggggcg ggtaaccgct cccctttcat atttagcgtg
    24121 cgggttggtg tcggatgcga tgctgatgca tcttatccgc cctaccatcc ctcccggcaa
    24181 catttattgc cgcttttgtt tacatattct gccgctaaac aattccccat tcctggcgta
    24241 tatctggcta acatttatca atgtgataga ttcctctccc gcatttatgg gaatgcgtag
    24301 tgacttattc taattatttt tataaaagca tccgtgataa tgaaaaggca tagaaacgtc
    24361 aatttgttat tgatgttggt attactcgtg gccgtcggtc agatggcgca aaccatttat
    24421 attccagcta ttgccgatat ggcgcgcgat ctcaacgtcc gtgaaggggc ggtgcagagc
    24481 gtaatgggcg cttatctgct gacttacggt gtctcacagc tgttttatgg cccaatttcc
    24541 gaccgtgtgg gtcgccgacc ggtgatcctc gtcggaatgt ccatttttat gctggcaacg
    24601 ctggtcgcgg tcacgacctc cagtttgaca gtattgattg ctgccagcgc gatgcagggg
    24661 atgggcaccg gcgttggcgg cgtaatggcg cgtactttgc cgcgtgattt atatgaacgg
    24721 acacagttgc gccacgctaa cagcttgtta aatatgggga ttcttgtcag tccgttgctc
    24781 gcgccgctaa tcggcggtct gctggatacg atgtggaact ggcgcgcctg ttatctcttt
    24841 ttgttggtac tttgtgccgg tgtgaccttc agtatggccc gctggatgcc ggaaacgcgt
    24901 ccggtcgacg caccgcgcac acgcctgctt accagttata aaacgctttt cggtaacagc
    24961 ggttttaact gttatttgct gatgctgatt ggcggtctgg ccgggattgc cgcctttgaa
    25021 gcctgctccg gcgtgctgat gggcgcggtg ttagggctga gcagtatgac ggtcagtatt
    25081 ttgtttattc tgccgattcc ggcggcattt tttggggcat ggtttgccgg acgtcctaat
    25141 aaacgcttct caacgttgat gtggcagtcg gttatctgct gcctgctggc tggcttactg
    25201 atgtggatcc ccgactggtt tggcgtgatg aatgtctgga cgctgctcgt tcccgccgcg
    25261 ctgttctttt tcggtgccgg gatgctgttt ccgctggcga ccagcggcgc gatggagccg
    25321 ttccccttcc tggctggcac cgcgggcgcg ctggtcggcg gtctgcaaaa cattggttcc
    25381 ggcgtgctgg cgtcgctctc tgcgatgttg ccgcaaaccg gtcagggcag cctggggttg
    25441 ttgatgacct taatgggatt gttgatcgtg ctgtgctggc taccgctggc gacgcggatg
    25501 tcgcatcagg ggcagcccgt ttaagctcac gtcaccgcag catcgtcatc agctccatgg
    25561 gagaacgatg ctgctttatc agatcacgca tcacccgcat atgcggcgca gagtaagaat
    25621 aaaacgcctg atagcccgca caaagcacgc tgtctggcat ctcctcccga gcgtgcggac
    25681 acagcagatg ccagtcgcac gcctggcagc cacggcgcgg ttgcgggttt tcgctgccac
    25741 accatttttg taaggtggcg tcaaaaagct caatcgagat acgactgata tctgcccgca
    25801 cccaacactc aaacagggca attaaaaatt tcccccacag ctctgctgtc accgaagcgg
    25861 ccgtcagatc gccccggtca tcaaattcaa gatggggaat gagctgtacg gagcggatac
    25921 cccgttgttt gaaattgtga taaaaatcgg caggttgcag agcatccttg tactctacaa
    25981 ccagtttatg cctgtcctct tcagcatcaa tgacctgact tcctgtcatg cgactctctc
    26041 aataaatacg ataagttgat gtatatgcgt tacagcaaac agtattgcga gagataacac
    26101 cgcgagggag atcaaaaatt tgtcacggac tacgtgaaat tgggaaaaat cgctattcgt
    26161 gacatccatt aaattacgat tacgggtgta gtgccagagg atcagcgcca cgatggcgag
    26221 gatgccaatc gaaatccaga ataacatgcc cgcctggtgc cagttgtgtt tgatagccaa
    26281 cgccatcaac gcgccgtagc ccagcatggt acgaaaccac gccagtgatg tacgctccgg
    26341 ctgcagtcct gggtcggcaa tgcgtctggc tttgcggcta tccggcatac aacaccagcc
    26401 ccataacgat caccgctacg accatcaata ttaagctgat gattaataag ctgttggtgt
    26461 agggcaggtc ctctttcaga cgcatcgctt tttcattgcg taaccagcgc aggtagccat
    26521 acatcgctaa cccgccggaa aacagacaca acaacaacgc cagcagttcg cgaatgacgg
    26581 gcgtggcgaa atctggcgca agttgatcaa gcccaacccc cgccgccaga aagcctagtg
    26641 cggtacggat ccacgccaga aaagtacgct cattagccag cgagaagcga taatcaggtg
    26701 cttctccgag gcgggaaatt ttcattgttg ctccttgtaa actgttcctc gccgaaaaag
    26761 taggccagga acagcaataa tgcatcagaa cttctgtttt ttctcttcca ccttcacccc
    26821 ttgtgtcggt aggccagtgt catagtcgcg tactactggc gaatagccat cttgtgggcg
    26881 tggacgaaat gcccccatcc agcgcggctg tgcatcttta cgccacggac gcaggctcca
    26941 ttgataactg cggaacggat cgcgaatttt gtccatgtaa tccaataagg cgtcatgcat
    27001 tttgctgcga acgtctgcaa aacggatatc atcgatcagg ttatgcattt catttgggtc
    27061 attacggcga tcgtaaagtt catcactggt gaagaggttg agcaccagtt taaagtcatc
    27121 cgtcacccag caacgcaccg gaataaaacc gccaaagctg tcatgctcaa tctcgtagcg
    27181 gttaaattcc accattacgc cgcgtggctc tttcactgca aggatatttt cccccagcag
    27241 aatctctggt ttttcaatat ctgccagcgc catcattgtc ggcagtaaat cgatatgact
    27301 gactggcgta tcgacctgtc gccgctcccc ttgcggcgaa cggatgatca gcggaatacg
    27361 ggtgatgtcg tcatacatcg ccgctccttt actgatcagc ttatgtgcac ccatcatttc
    27421 accgtgatcg gaggtataaa tgacccacgt attttcacgt tgctctggcg ttaaggcatt
    27481 gatgacccgt ccgatttggt catcaacaaa gtcattgcag gcaaaataga gcggatggtg
    27541 ataaagcccg tcatcaccta ctggcgatgg catcgcctgc gcccataagc ggtgatgttc
    27601 cggtttgttc gccaggtcat cctgtgattt ctcgccaagt tcgtagtaaa aatcagcgta
    27661 tttctctaaa tactccaccg gacaggtgaa cgggtgatgc ggctcatcat acgaaaccac
    27721 catcaggaag ggttcctcgg cgcgcgcggg ctgttgcaga aaatctaccg cccgattgct
    27781 gatgcggtgc gcccaggtga aggtttcgtc gatatggttc gcctgtaaat cctcaacgct
    27841 gtttaggcca ttgcgccaca ggctgatctc tttttccgtc agttcgctaa ggtagttcgc
    27901 cccatcgaac cagtaatcag cgtcccactc cggcggacac tcgccagtgc cgaaatagtc
    27961 atgaccgtcg agatgccatt tgccgatgta acaggtgtga tagccggcat ccttaaagta
    28021 gcgtcccata gtggagatgt ttttgcccgg cgcgacgttg ttggtccacg ggccggactg
    28081 gttagcgtag ataccggtaa acagcccggc gcgcgctggt gtacaaaccg gtgaacaggt
    28141 gtaggcggaa ttaaagcgaa tactttccgc cgccagacta tcaatatttt gcgtattcag
    28201 cggcttaccg ctatagcaac cgaccatatt ggtggcctgg gtatcggtca tgatgaacag
    28261 aaaattgggg cgtttcatag gctatccttc gcgtcgggag taaaggttac ggctgggtcg
    28321 tgacgatgcc gccagctgtc aaaaatcagg tagacaatca ctacggcagc gatgaaataa
    28381 ctgatcatcg ctaaccaacg cgtaccgtag ccgccgaatt cagccagccc ggcatagacg
    28441 ccaatcatcg cgaacaggat gccaattgac gcgatcttga cgtttttcca cggtttcatg
    28501 tccaccgcaa acgcatcttt gaaggtgaac ggcgtggcgc gcggtttgag aaaaccgatc
    28561 accagcatca cgaccacgtt gatgcagaac gtacaggcca gcacatagag gaaatggaag
    28621 tcgaacttca ccagatagtt gatggtgatg tagctgatta tgccaatccc catcgccact
    28681 tttgccgcca gcgccgggat gcgcgggaag aaaaagccca taatgatgat ggtaaccagc
    28741 ggcacgttgt aaatgccgtt gagctgtttc atccagctat acagcccctg cggcgcgttg
    28801 gcgatccacg gcgctaccag caccgaaacg atagcgataa agaaaccaaa tttgcgcccg
    28861 acggtgacca gctgttgcgg ctcggcattc tggttaatga tgcgacggta aatacccata
    28921 ctgaataacg tactggcgct attcagaaag ccgttgaagg tactgatcac cgcaccaaat
    28981 aacaccgcgc cgaagaaacc caccagtggc actggcagaa cgttattgac cagcgtcggg
    29041 taggccatgt cggctttcgg tagatcctga tacagatgaa acgcaatcaa ccctggcagc
    29101 accagtacca gcgggtcgag cattttcagc accgccgtta acagcgcccc cttttgccct
    29161 tccgccaggc tttttgacgc cagcgtgcgt tgcacgatgc cctgatttgt acaccagtaa
    29221 aaggtgttca ccagaattaa accggtaaat gccgcgccaa tcggcaaggg atcggtcggg
    29281 ccgccgactg agtttaattt ctcggcgtga acggtggtga gttgctcaat gccctgcata
    29341 aagctgccct tgcccatcgc gatcaggcca aatatcggca ccatcaaccc gccgataacc
    29401 agaccaatac cgttaatgga gtccgccact gccattgcgc gcagtccgcc gatcaccgca
    29461 tacaaaattc ccgccagacc aagcaaaatt accaatagcc agatagccgc accgtgagaa
    29521 atctgtagcg attccccgac gtgaaacagg ctgttgagcg ccaacgcgcc ggagtagaga
    29581 acaatcggca gaaagcagac gccggtggca atgaggaagc agaagtcgat gataatacgc
    29641 gtcgttttat cataacgttc ctccagaaaa tcggggatgg tggcaatgcc gcgcttcaga
    29701 tagcgcggta aaaagattag cgcgaggaag atcagcgtca ccgctgaagt cacttcccag
    29761 cccatcaccg acatgccgct tttgtaggcc tgcccggaaa gaccaaccag ttgttccgtg
    29821 gaaaggttgg ttagcattaa cgaagcggca ataaccggcg cttttagtga acgtccggca
    29881 agaaaatagc cttgttgcga tccggtatct gttttgcgga ccttccacca ggtgatcacc
    29941 gccaccagca gcgtaaaacc gacaaaactc aagatttgta acgaattcat ctcatagccc
    30001 ttatttatgt ttatgtcccg gacgcatgaa tatagggaga ggtaaaatgc gtcgggtcag
    30061 gcagtcagat tcttaaaata caaacatcgt atccctgaac ggattgtttt ctgttaagtt
    30121 caggttgtga gcataattcg ccggagggat attaaaatga atggaaactt gcaaagttcg
    30181 gatgtaaaaa acgaaactcc gtacaatatt cccttattga ttaacgaaaa tgtgatctcc
    30241 agcggaattt ctctgatctc gctctggcat acctacgccg acgagcatta tcgggtgatc
    30301 tggccgcggg acaagaagaa accgcttatc gccaactcat gggttgcggt gtataccgta
    30361 caaggatgtg ggaaaattct tttaaagaat ggtgaacaaa taactctgca tggcaactgt
    30421 attatatttt taaagccaat ggatattcac tcttatcact gtgaaggttt agtctgggaa
    30481 cagtactgga tggaatttac ccccaccagt atgatggata ttcccgttgg tcagcaaagc
    30541 gttatttata atggcgaaat ttataatcag gaactcaccg aagttgctga gttaattact
    30601 tcaccagaag aaataaaaaa taatctggca gtcgcttttc tgacgaaaat tatttatcag
    30661 tggatttgtc ttatgtacgc agacggtaaa aaagatccac aacggcggca aattgaaaaa
    30721 ctaattgcca ctttacatgc cagtctgcaa caacgctgga gcgtagctga tatggctgcc
    30781 acgatcccct gtagcgaagc ctggttgcgt cgtctgtttt tacgctatac cggcaagacg
    30841 ccgaaagaat attacctcga tgcacgtctg gatctggcgc tatcgctatt aaagcaacaa
    30901 gggaactcgg ttggcgaagt cgctgatacg ctcaacttct tcgactcctt tcatttcagc
    30961 aaagccttta aacataaatt tggttatgcg ccgtcagccg tgctaaagaa tacggaccag
    31021 cacccaacgg atgccagtcc acacaattaa cgtaactctg gccagtacgc tttgttggcc
    31081 tccagcaaat catccagaat agctttggcg accgaggcgc tcggtaccgt tttcgacaac
    31141 gtaatcgcct gccacaggtg ctgatatgaa cgctgttccc aggcgtccac gaccagtttt
    31201 tccaccgcca cttgctgact cattaaccct ttctgaaact gcgggatatc gccgaccacc
    31261 agtggttctg gtccgttgtg cccgaccaga cacgggatct cgaccatcgc ttcatcatca
    31321 aagttatgga ttgccccgtt gttaggcaca atcagcaaca tccgctcctg agtgttgaag
    31381 gcaattgccg ccgccagatc gacgatgtat gacgcatgtt cgtcaatttc cagcttgccc
    31441 gccgctgaat ttcctgccgc cgtaatggcg cggcaagcat cgaaaacctg tttctcgcga
    31501 tgttccatca cctcattcgc gcgggtatgt tcagggttgg aatgctgaac gacataatcc
    31561 gggaagagat aatatttcag ataggtattc ggtaatgtat ccgggtccag agcctggaca
    31621 tcccgcgctt ttgcataggt gtcgttccag ctcgcttcag tatgtgtatc ttgctgcggt
    31681 ggaacataac cgtgcttaga gacatattgc cgcagctggg gcatcaggtc gttgccctgc
    31741 aaatcttcaa ttgatgtcca ccagccaaag tggttcaggc cgtagtagcg cacgcgcata
    31801 tgtttgcgat cttgcagccc aacaatttgc gccatccggc tttcaatacc gattggcatg
    31861 tcacagatgt tgaggatttt cgcattcggg cgcagacgac gcgtggcttc tgcgacaata
    31921 gctgccgggt tggagtagtt gagcatccag gcatttggtg aatatttttc catataatcc
    31981 accagttcca ggacgccgcc aatggaacgc atgccgtacg ctattccgcc gggtccgcaa
    32041 gtttcctgac caacaacgcc gtggcgcagc gggatttttt catccagttc gcgcatcggg
    32101 tatttgccga cgcggatgtg cgccatgaca aaatcaacgt cgctgaatgc cacttcagga
    32161 tcggtggtgt aactaaacgc aatgtccggc gctttttctt ttaggatgac tttacaggct
    32221 tcggcaatca cttcctgccg cgcgccatcg ttatcataaa atttcagtgc acgaagcggg
    32281 aaacggtcct gattcgccag gagcatcaac acgatgcctg gcgtaaaggt gcttccaccg
    32341 cctgcgacaa ccactgagaa tttggtcatt atactgcctc cgtaatggca acattttctg
    32401 ctgattgatg agaattaatt aagctatcga gctgttcacg cagctgggat acatgcagac
    32461 caatgatcac ctgaatggca tcgccactac ggaagacgcc gtgcgctccc agctttttaa
    32521 agacttcgtc atccagcgtt tgtgacatgt catgcagtgc aatccgtaga cgcgtcgcgc
    32581 aattgttgat gctggagata ttgccgaccc cgcccagggc ttgcaggata ccggcagcct
    32641 gatccagctc tttttttggc tctgcagcgg tggtttggcc tcgcgaggct ttgtattcgg
    32701 cttttgagta gagtttcact tccgcatctt cacgtcccgg cgtgcacatg ttgaattgca
    32761 gaatcagggt gcggaaaacc acgaagtaca gcagggtaaa gcacaaccca atggcgattt
    32821 gggtcagcat catatccgcg tggttgctga acatcgggat ccagttttgc ggtaaaacct
    32881 ggtcaatcaa accaccgccc atgttgccca ccacaccaaa gagatacatt acggtcgaca
    32941 ttgaggccgc cagcacggcg tgtactgcaa acagcaacgg tgaaatgaac aggaaggtaa
    33001 attccagcgg ttcggtaatt cccaccagca tggcggttaa ggttgcggga atcagcaagc
    33061 ccgccacttt tacccgattt tccggtgcgg cagtgaagta catcgctaaa gaaatgccca
    33121 cggcaccaaa aatttttgag ttaccgtgca gggcaaaacc gccttccggg aacaacgatt
    33181 tcagcggctc ggcgctcaaa ctgaactctt gcagatgctg cgcccagtac atctgaatgc
    33241 cgccttcaac agctgccgga ccaaagataa acggtccgta gatgaagtgg tgtaaaccgg
    33301 ttgggatcag aatacgttcg aggaaggtgt aaacccagac cccaagcgca cccgccgaac
    33361 gcaggaacgc ttgcagagat tcaattccca tttgtacttt tggccagccg agcaacgtca
    33421 gccaggcgca ggggatcatc accaggaagg cgataatcac cacataagat gtcccctgga
    33481 aaatgccgag aaacaccggc agttttttat cgaacagacg gttatgcagc gccgtcacaa
    33541 tgccggaaat gataattgcg ccgataatgc tggtatcgag ggttttaatc ccggccatca
    33601 ttgtcagacc gctacctgcc accgcgtcct gagtgaaatc gacgccgaag tagcttcccc
    33661 aggtcattcc catcgcgttg atgaaatagt tccaggtcag gaaactcacc atcaccgcca
    33721 gacaagctcg cccctgcgct tgcttagcaa ggccaatggg taaaccgaca gcaaaaatca
    33781 gcggcatatt acggaatacc gtccaaccgc cctcttcaat aatatgtacg atttgcgcga
    33841 ataaactgtt cggatcggtc agtgattccc cgacaaacat cgggttttgc agcaagatgg
    33901 caagacccac cacaatcccg gcgaagggaa acagcagcac tggcgtgaac atcgcgccgc
    33961 caaagcgttg aatttgactg agcatttcca catcctcttt tctcaattct gtaagtagga
    34021 agcctgattg gttatgcggc aggcctggag cgaggatagg aagatgcgat cggcagaaca
    34081 tgacgaccca gtgggattcg tgatcgcttt catgcttttc gttgggcggt agagatctac
    34141 gttttcgcaa aaaagtagac atctgtgagc actggtacac agcaacgaac acatctttaa
    34201 aaaaaagatg ttttttcaat cgattaagca gaacttgtgg gcgcattacc cgggcttgca
    34261 ggcaaaaaag agatctagag atgatctaca aaagcattgc ggagcggtta agaattcgac
    34321 ttaattccgc agatttcacg ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag
    34381 agtttgcggt atcgcggatg accatccgta aagccattga cctgctggtg gcgtgggggc
    34441 tggtggtccg ccgccacggc agcggcactt acctggtgcg taaagatgta ttgcatcaaa
    34501 ccgccagcct gaccgggctg gtggaggtgt taaaacgcca gggaaaaacg gtcaccagcc
    34561 aggtgctgat ttttgaaatc atgcctgcgc ctccggccat tgccagccag ttacggattc
    34621 aaatcaacga gcagatctac ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctga
    34681 tgctggaaga cagctatatg ccggtaaaac tgttccgtaa cctttcgctg caacatctgg
    34741 aagggtcgaa gtttgaatat attgaacaag agtgcgggat tctgattggc ggtaattatg
    34801 aaagcctgac gccggtgctc gccgatagac tgctggcgcg gcaaatgaag gtagcggaac
    34861 acacgccact actgcggatc acctcgttgt catatagcga gagcggggag tttttgaatt
    34921 attcagtgat gttcagaaat gccagcgaat accaggtgga gtaccattta cggcgactcc
    34981 acccggaaaa gagttaaccg atactccaga agagcaccgc cagtaattgg ggggtgataa
    35041 tgcgcaggaa catcactaac ggatagacag tggcgtaaga gagcgccgct gcaccgctcg
    35101 ttggatgaag attattagca aatgccagcg ccggtggatc ggtcatggag ccagccagca
    35161 tcccgcacat ggtcaggtaa ttcattttgg ctaacatccg cgccagaata ccaacagtaa
    35221 tcagcggaac ggcggtgatc agggcaccat atccaatcca gcttagccct tcgccattga
    35281 ccagggtatt cacaaaatcc ccaccagatt tcagaccaac gaccgagagg aacagcacga
    35341 tccccagctc ccgcagcgcg aggttggcac ttggcggcat aaaccagtac agcttgccga
    35401 tactgccgat acgcccgagg atcaacgcca taatcagcgg accgcccgcc agccccagtt
    35461 tcaacgcggc cgggaatccc ggcacaaaga cgggaataga acctaacaat acgccaagcc
    35521 cgataccaat aaacaccggc agcatctgaa cctgttgcag tttttgctgc gcattcccca
    35581 gcacattggc aacggcatca attgcggacg gacgccccac caggttgagg atatcgccga
    35641 actgcaggct gatatcgcca ctggcaacca gttcgacccc ggcacggttc aggcgcgaga
    35701 taacaacgtc atagcgttct ttaaagtgca ggtcgcgaat acgcttacca agcacgtttt
    35761 cattggtgac caccacacgc tcgacgcgta aatcagtgcc tttcgtggac agcgaggtat
    35821 cgacctcctg accaatcacc agttgcgcat tatgtaaatc cgctggctgc cccaccagat
    35881 gcagcaaatc gcccagttgg ataatggtat ctggcgaagg tacttttagg gtttcttcgc
    35941 gtttcagacg cgagcagata attttgtcgc cgttgagaat cggcacatct ttaatggcta
    36001 aatcatgcag gttagggttc tcaacgcgaa tattgatagt cctgatcagc gcgccgccat
    36061 tggtgcgaga agactcgtgc tgctgggctt ctgtctcgac attgacgcgg aaaataaccc
    36121 gcaacatcca catggtgaac aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac
    36181 tcatccccat ctgatcgacc atttccattg gtgtacccag gtcgcgcaag atctgctgcc
    36241 ctgcccccag cgctggcgta ttggttaccg caccggagaa aatccccagc actaccggca
    36301 gtggaatatc aaacagttta tgcaggatgg cggtaaccag acccccgatg atgacgatca
    36361 gaacagcaaa caggttgagg cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc
    36421 ctacctgaat cccgatggta taaacgaaca ggatcaggcc aaattcctga ataacatgca
    36481 gcatatcgct acttaatgtc atccccgcct gagaaacaaa atggccgacg atgatcccac
    36541 caaacagcac gccgccaata cctaatccta tgccgcgaaa tttgacgttg ccgataaaca
    36601 aaccgacgac tgccaccaaa gccagaatac tgaccgttaa tgctatatca ctcatcgtcc
    36661 atttctcttg agataacatt ttaattgcta cggattatgg ctgaatcggt gaagcagatt
    36721 tgccttatag cgcacattat cacgggaaag aacgtgccga aatatcttaa acaggcgaaa
    36781 tttaacgcat tgttgaagaa gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga
    36841 gaatagctag ttagctattt aacgcgggac gttcgctgat agcgatacgc tgcgctgcga
    36901 tgggttcagg ctcattacga attaaatcaa tatgcagtaa accgtttacg aaggttgcac
    36961 cagagacttc catattttca gccagcgtaa agctcaggct aaatggctga ttcataagcc
    37021 cttgatgcag ccattttttc tcttcttttg gctgctccgg cgtgcctttt acgctcaggc
    37081 gcgtaccttc cagttgaatc tctaaatctt cctgacgaaa acctgccagc gcaagggtaa
    37141 tgcggtagtg gttatcgtcg cttttctcaa tgttgtacgg cgggaagctc tggctttcac
    37201 cggcgttttg cagtgcgttg gccagtttgt caaaaccgat ccattgacgc atcagtgggg
    37261 ataaatcgaa gttacgcata gtcatttctc cttctaagaa gcgagtaagt acctgcaaat
    37321 ccgaagattc gcatatgctc cctgacggcg agcatggaga tgtcaggccg cgccaggcgg
    37381 ccttagggaa ttagttgatt tcgatacggc gcggtttttt cgcttccgga atcacacgtt
    37441 cgagatcgat atacagcaaa ccatttacca ggttagcacc acgaacatga atgttctcag
    37501 ctaactggaa tttgcgttca aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct
    37561 ctttttgttc gtcggcgtga gcacctttca ccaccagcag attatcctgg gcggtaattt
    37621 ccagttcgct ctcagcaaaa ccagccacag cgatagcaat gcggtaatgg ttttcgtcta
    37681 ccagttcaac gttatacgga gggtagccgc cattactctg gctctggttg ttttctaagt
    37741 ggttaaacaa acggtcaaat ccaatagcag aacggtaaag cggggataaa tcaaagttac
    37801 gcataatcaa tagctcctga aatcagcgag aatgtaagac cttccacaat ggacaggtca
    37861 ggtagccaga acacccatca ggcgcgtcct catcggctac gatgtaaaaa tgggtctgga
    37921 aatgaacttt tcaagcctta tcagcggact tttttttgca gtttatggtc tattgcatag
    37981 actgaggggg cagcacgaaa cgttaaagtg cgatagccgc cacagtgcga cgtaatgatg
    38041 atgttatttt cagcaaggga tcgctataac tcatcaggcc aacacccacg atcaacagat
    38101 gaatttatta tgataagaaa tgtgttgtta gcgttcatga tatgcagcgg aatgacatta
    38161 cttggaggat gctccagcgt gatgtcccat accgggggga aagaaggaac gtatcccggc
    38221 acccgcgcca gcgccaccat gattggcgac gatgaaacca actggggcac taagtcgctg
    38281 gcgattctcg atatgccgtt taccgctgtg atggacacgc ttttgctgcc gtgggacgtg
    38341 ttccgcaaag acagctcggt gcgctcacgc gttgaaaaaa gcgaagccaa cgcgcaagcc
    38401 accaatgcgg ttattccacc ggccagaatg cctgataact gatttagcga tcagtttcgg
    38461 tgatccacag ctggccgcct tccatccacg ccagccattg accatccggc gagaagacca
    38521 cggcgtccgc agaaggtgga ttggcgtggt tttcggttaa atactcaacc tcgccgcttt
    38581 gcgcatgggc acaagcaatt cgattatcca gcacaaagcc caaccattct cctgatggat
    38641 gccagttaaa tgcagactga atatccgttt tgttatgggt taactggtgc ggctcgccgc
    38701 cctgtggcga gataagccac agttgcacaa tgccgttatc atcacgcatt aaaaacgcga
    38761 tttgcgtacc ctgcggatta cagcgcaccc agtggcgcgg gacgttgact aaccccggat
    38821 aagcccgatg gtgggtaaag gttaaacgtc gctgcacgac gccacgcggt ggcgcgggca
    38881 gggttgtttc cgttccactt aacggcgcat cacctgctac tttccagcca gcttcatctt
    38941 gcggtaactc aacgataaac agctccggca ctttctcgcc ctttggcgaa agtgtatcgc
    39001 caataaacgc cagcgcgtga tttcctaccc atccttcttc ataagcacga ttgatttcat
    39061 cactgccagg ctgtggcgtg ggcgtggttt tacttaccag cacgcaccag tggctaccgc
    39121 tgtattcacg cggatgctgc ttttgtacgt tgaccgggcc aaacggcgca gcaacaccga
    39181 cgtttcgcaa atccagcgtc ggggagagtt catgcattac atggtcgtta taggtaaagc
    39241 tcaccctttc accgttcggg ctaaagacat gtacatgact gccgccgcgc agcgcccctg
    39301 gcgtatacgg cgcggtaata tccatcgcat caaggttact caccttgccg ccttcaacaa
    39361 tcactccgcg ccgatgatgg aagtcgtaat accatgtttc atcaggattt tccgggccgt
    39421 ggatgaaaac atatttctct gacttcggat gaacggtcac cacgccgacg tacgcgccct
    39481 gtgacgcgcg atagataacc tcgacctcgc cggtatggat attcacacgc tcaatggtct
    39541 cgccggtaaa cgacgcgcca gaaggacgca cgtcaaatac cagccactgg ctgtcgggcg
    39601 tccaggtatt ggtattggtg agcaggtgat tacggggagc aaaggttatc tgtttcatgg
    39661 cgcggtcctg atacaaaacg ggtcccgcca tcatacttca caaggacttc actttcagac
    39721 aatagtcttt cgccatcgtg atgaatgtga gtgaaaagat ggaacatttc gacgtggcga
    39781 ttattggcct cggcccggca ggttcggcgt tggcacgaaa gttagcaggc aaaatgcagg
    39841 tgatcgcgct ggataaaaag caccagcatg gtactgaagg tttcagcaaa ccttgtggcg
    39901 gtctgctggc accggacgcg cagcgatctt ttattcgcga tggactgacg cttcctgtcg
    39961 atgtaatcgc caatccgcag attttcagcg tcaaaaccgt cgacgtcgcc gcatcgctca
    40021 cgcgtaacta ccagcgaagc tatatcaata ttaatcgcca tgctttcgac ttgtggatga
    40081 aatcgctgat ccccgccagc gttgaggttt atcacgacag cctgtgtcga aaaatctggc
    40141 gtgaggatga taaatggcat gtcatttttc gtgcagacgg ctgggagcag catattaccg
    40201 cccgctatct ggtcggtgcc gatggtgcca actcgatggt gcggcgatat ctctacccgg
    40261 accatcaaat tcgtaaatat gtcgctatcc agcagtggtt cgcagagaag catccggtgc
    40321 cgttctactc ctgcatcttt gataatgcga taactgactg ttactcatgg agtatcagca
    40381 aagacggtta ttttatcttt ggcggtgctt atccaatgaa agacggtcag acgcgtttcg
    40441 cgacgctgaa agagaaaatg agcgcctttc agttccagtt tggcaaagcg gtgaaaagtg
    40501 agaaatgtac cgtgttgttt ccctcacgct ggcaggattt tgtctgcggt aaggacaatg
    40561 cctttctgat tggtgaagcg gcgggattta tcagcgccag ctcgctggag aggattagct
    40621 atgcgctgga tagcgcagag attctgcgtt cggtgttact gaagcagccg gagaagagca
    40681 acgccgccta ctggcgcgcc acacgcaaac tgcgtttaaa actcttcggt aagatagtaa
    40741 aaagccgatg cctgaccgca ccggctttaa gaaagtggat tatgcgcagt ggtgtggcgc
    40801 atattccaca gttgaaagat tagccaacgc gcttcacatc gcccaccagc aggatgtaag
    40861 agagcgcgcc aatcaacgcg acggcggaga tataaaccag tgcaggtgcg aaaccgtaac
    40921 cctgcgccag gtaccccacc accagcggaa cggtgatgcc gcccagacca ccggcaaagt
    40981 taaacacgcc gccggttaaa ccaatcaggc gcatcggtgc cagagaagag accagcgacc
    41041 aggtaatcga agcaaaaccg ttgccgaaga atgccagcgc catcaggcac ataatcatca
    41101 tcggatcgtt ggtgtagtta gcgcccataa tgcaggtgga gatcagcaag ccgcagataa
    41161 tcggcgtttt acgcgcaaag cccagtgaaa agcccttacg taccagcaga tccgcgaccc
    41221 agccagagag caggacgccg acaaacgccg cgaggaatgg caccgtggtc ataaagcctg
    41281 ctttcagcgc cgtgattcct ttttcctggg ttaaatagtt cgggaaccag gttaagaaaa
    41341 accacagtgt agaagccacc gcaaattgcc caagataaac gccgatcagt ttacgatgga
    41401 acaccagctt ccagtctttg gctgttaacg gctgacgtgc ctcttttttc accggcgcat
    41461 caccatccac cagaccgccg ccatcacgaa tgtaatccag ttcagctttg ctgatacctt
    41521 tggtcaggcg cggcggctga taaaccttaa accaaatcag cgaccagata atgccgatac
    41581 caccagtgac aatgaacacc cagtgccagc tcaacatttc ctgaatccag atcagcagcg
    41641 gagtcaaaaa cgccagaccg acaaactgac cagacgtata aaaaccaacg gcggaagcgc
    41701 gttcatgttc cgggaaccag ctggtcacca tccggttatt ggtcgggaaa gcgggcgctt
    41761 cgaaaatacc ggttatcgcg cgcagaccaa ttaatgacat taatcccgtg gcaaagccct
    41821 ggaataaagt cgccactgac cagccaaata tcgcaataaa ataagtcacg cgtgaaccta
    41881 cgcgatctaa gaaccaaccg ccggggatct gacatagcgt ataaagccag gcgaaggccg
    41941 aaaatacata gcccatttcc gctttagtaa taccaaactc ttcctgaata tgggcagaag
    42001 ccacggccag gttagcgcgg tcaacataac aaatgaccac cgtaataaag atcatcacca
    42061 gcgtcagata acgccgacgc cccggctttg ctgcattaac gggaatatcc atagcgagct
    42121 ttctccagat tttgggcata gcgaagccgc tcaccatgcc ctgtaattta cagagggtta
    42181 tatttttgta ttgctgtttt agtgcccgat gaggggctta cgtggcagga attaccactc
    42241 tgctacgctg ttatcttcat gacgccagag cggattacgc cagtccgggg catttttact
    42301 gaactcaata actttagctt cgtcgatttc cacgcctaag cccggtttcg ttagcggttt
    42361 aaagaagccg ccaaccatgc tgaagtcttc tttgtttttc acaaagtcga gtaactccgc
    42421 gcctttgttg taatgaatgc ccatactttg ttcctgaagt acggcgttat aggaaacaaa
    42481 gtcgatatgc agacaagccg ccagtgcaat cggtccgagc ggacagtgcg gcgcaagggt
    42541 cacgtcatag gcttctgcca ttccggcaat tttgtagcat tcggtaatac cgcctgcatg
    42601 agagagatct ggttgcagaa tcgaaatacc gcccgcctcc agcacgcgtt taaaatcgaa
    42661 gcgtgagaac atgcgttcac ccgccgccag tggaatatgc gtttgtgccg ccagtttcgg
    42721 gtagtattcg gcttgttccg ccagcaccgg ctcttcaata aacagcgggc gatacggctc
    42781 cagttcttta atcagcactt tcgccatcgg cgcgctgacg cgaccgtgga aatcaagacc
    42841 aaactcaatc tgattgccaa aagcttcacg aatttgtgcc acggtgttga ccgccgcatc
    42901 taccgcgcgg gagttatcaa ttagccccag ttcttcacaa ccattcagtt tgaaggtatc
    42961 gaagccgatt tcgcgcaggg tttttatgcc gtcgataaca tccgccggac gatcgccgcc
    43021 gacccaactg taggctttaa ttttgtcgcg aaccaggccg cccatcagtt gccagaccgg
    43081 cgcattcagc actttgcctt tgatatccca taacgcctgg tcgatcccgg cgatggcgct
    43141 catcaggatt ggaccgccac gataaaatcc ggcgcgatac atcacttgcc ataagtcatt
    43201 gatgcgcgat ggatcctgac caatcaaata gtcacccagc tcgtgaactg ccgcttccac
    43261 cgtacgggcg cggccttcga tcacgggctc gccccagccg accacgcctt catcggtttc
    43321 aattttcagg aacatccagc ggggaggtaa acgatacgtg gtaattttgg tgattttcat
    43381 tgcactgcct ctcgatacgc cttaacaaat gctgctgcct gctgcgcggt gcgttctacg
    43441 gattgcccgg cgcgatagag atcgctgcct aagcctgctc ctgcacaacc tgcgtctatc
    43501 cactgcgcca ggttttctgg cgtcacgccg ccaacggcaa agactgcgat gtccggtggt
    43561 aataccgctt ttaacgcttt gatgtattgc ggaccaaaag ccgatgacgg gaatattttc
    43621 agcgcctgcg cgcccgcgtc gagcgcggta aaggcttcgg tcgccgtcgc gcagccgggg
    43681 caaacggtca taccgtagcc caccgcacgg tggatcactt cactatggat attgggcgta
    43741 acgatgagct ggcagcccat cctggcgagc acatcgacct gttcaggttt cagtaccgta
    43801 cctgcgccaa tcaacgcctt gtcgccatac gcatcaacga tggcgggaat gctctgctcc
    43861 cattgtgggg aattcagcgg gatttcaacc gcgtcgaacc cggcgtcaat caccgcgcca
    43921 acatgcgcca gcgcctcgtc gggcgtaatg ccgcgcaaaa tggcgatcag cgggagttta
    43981 gtttgccact gcatgagcga tgctccttat accagcctga aatgccgtgt cgcccgctac
    44041 cgccgccacg tcgcaaccca tcgcctgaaa ggcttgctgg tagcgcgcgg tcagcgatgt
    44101 tccggcgaca agggtgatgg cgtgttgatg ggtcacatag tcgcgcatac tggcaacctc
    44161 tgcgccaatc aacaaaccag agagaaattc actgacctgt tcgcggggaa gcgttcccag
    44221 cacatgcgag gcgcgaactt caaaaagctg cggcaatatg gcgggagcat taaggccacg
    44281 ctcaaggcca gccgcgaagg catcggcaga gttttcctgc agcggcaaac ctgcgccaat
    44341 caatgagtga tttaacagta aatgatgtaa ttcaccggtc attacggtgc gaaaatcgtt
    44401 gatttgctgg ctatcggcct gcacccattt gcaatgcgta ccgggcatga cataaagaga
    44461 ggaaggagcg agagcacgcg cgccgatcaa ttgtgtttct tcgccgcgca tcacattgtg
    44521 gttatcgtca tgagagacgc ataatccggg aataatccaa atattgtcgc caaccgacgt
    44581 tgattgatcg ccaatagacg aaaaacgggc aggaacagat aaatacggtg caactttcca
    44641 gccgacattg ctgccgacca ttcctgccat caccactggc gtattctctt cacgccagtc
    44701 ggtcgtgact tctgctaaca ccgcagccgg agattttccg ttcaggcgcg tgacgcctgc
    44761 ttctgattgc ctgctgtcca ggcagtggtc gccctgataa agccaggcgc gcagattggt
    44821 cgatccccag tcaattgcga tgtagcgagc tgtcatgtga tttcctttaa ccttcgtgtc
    44881 gagctggcga tcatggtaag cgccgcctgc tctgccgcat cgccgtcctg atgccgtatt
    44941 gcatcgaaca gcgccttatg ttcctggagc gtttgcggca tgttggcctc atcgcccatc
    45001 caggttcgtt caaaaaccgc ccgctgcagc gaactgatcg caatgctaag ttgctgtaac
    45061 accgggttat gcaccgactg cagcaccgcc tcgtggtagc gaatatccgc ttcgttaaac
    45121 gcttcgcggt cctgattgtt ggcaatcatc tcgttcagcg acgattcaat ctgcgccaga
    45181 tcgctggaag tcgcgcgttc tgccgcccaa cgggcaatcg ccggttccac cagatttcgc
    45241 acttcgctca tggcactgat aagccgtagg tcgtagtcgt tttccagcac ccattgcagt
    45301 acgtcagtgt cgaggtaatt ccactggtta cgcggtgcca caaacgcgcc gcgataacgt
    45361 ttcatttcaa tcagccgctt cgccatcaac gagcggaaca cctcacggat gatgttgcgc
    45421 gaggttgcaa actcctcaca gagttccgcc tcagccggaa gcggcgagcc tggcacgtat
    45481 ttgccgtgaa cgatctgttt gcccagcgta atgacgatgc gatcggtttt attgagagtc
    45541 atggagagtc cttgtgctct gtatgttctt ctctacttta ccccgatcga tgcataacgc
    45601 ggcaactttg tagtaccagc gtgatgacgt tcgcgtttgc tgtgtgtgta atgtagtaca
    45661 aacttatatt gttgtactac aatttagatc acaaaaagaa caatgcataa aaaaatgaca
    45721 tgcgtcgggc agaaatctga aaagggatgt caggcgctaa acaggaggga aagaagagta
    45781 tgctttcaac ggcttagcta ctcgtttaaa ggattaatca tgaagttgaa ttttaaggga
    45841 ttttttaagg ctgccggttt gtgttctttg gcatttgcgt tgaccggatg tatcacctgg
    45901 ggattggttt ccaatactgc aagccgtcca catgccgagc aatggcggtc agacacgatt
    45961 aagggtttat cgctggctga agacagtaac ggtacgaaag gctatgtttt tgttggtgaa
    46021 tcattggact atctgttgac caccggcggc ggtgaggtgg ttaaaatgct gaacgatccc
    46081 gcaattcatg gtgaaagaat caccgtttct gataacgcga aatttatttt aagcagcagt
    46141 aataagaaat tctctggtgc tattacgctc tattacgact ggaacaacga agaagataag
    46201 gcactggcaa cacaatatgg ttttatttgt gatacccggc gttgtacatg gatgctggac
    46261 ggtttgaaag gctcaatcca ccagaaaaat aaaaaagcgg attactcaaa cgtgatggtt
    46321 ttccatcaac cctttacggt gggattttat gaatacaaag caacagatgg cgtcccgcgc
    46381 ggcctcgtta atgcgctgct gccagtcact cttacgctgg atatagtaac gtcaccactc
    46441 cagttcctga ttttatgcac gaccagaaat tgttaatcgc tgcgggcaaa cgcattcatg
    46501 ccgtttgccc gttcactgat taattcagca catacttctc aatagcaaat gccacgccat
    46561 cttcaaggtt ggatttggtg acaaagttcg ccacttcttt caccgaagga atagcgttat
    46621 ccatcgccac accgacgcct gcatattcaa tcattgcgat gtcgttttcc tgatcgccaa
    46681 tcgccatgat ttcttccggt ttaataccta acacgtcggc cagtgatttc acacccgtac
    46741 ccttgttaac gcgtttatcg aggatttcga ggaaatacgg cgcacttttc agcacggtat
    46801 atttctcttt cacttcctgc ggaatacgcg cgatagcctg gtcgaggatg gcgggttcat
    46861 caatcatcat cactttcagg aactgggtat tggggtccat tttctccgct tcgcagaaca
    46921 ccagcggaat ggtggcaacg aaagattcat gcaccgtgta gtagctgata tcacgattgg
    46981 cggtgtacag cgtggtgcgg tccagcgcgt ggaaatgaga accgacttcg cgggagagtt
    47041 tttccaggaa acggtagtcg tcatagctca gagcggtttg cgccacagtg ctaccatcgg
    47101 cggccttctg taccagcgcg ccgttataag taatgcagta gtcgcccggc tgttccatgt
    47161 gcagctcttt caggtaatta tgaacacctg cataagggcg acccgtcgtc agcacgacat
    47221 ttacgccacg ggcgcgagct gcggcaatcg catttttaac ggcgggtgaa atggtgtgat
    47281 cgggcagcag aagggtgcca tccatatcga tagcaatgag tttgatagcc atgagttccc
    47341 ctggtagatt ggttcctgac ccatgctaac gcgattccgc tcaaaaatca gtacaacacc
    47401 ctggggaaaa gggggatgca acgcgcgttg cgtgctcccc atttgcttag cggaagagtt
    47461 tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt ggtgacactt
    47521 caccttgcgg tgagagtgca tcaataatct tcggcaattg ttctgccagt aaactggaag
    47581 ccgtactggt atccacgccc agtttttgcc cgaggtcgga caccgcattt gtgccgagcg
    47641 ccgattccag ttgctcgccg ctaaccgatt gattgccctg ttgattactc agccaggttg
    47701 agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga atgccgccct
    47761 gctcctcaac ccagctcaaa atggcctgat atttccccgc atcgcctttc agaaaggcac
    47821 cgacaacttc atcaaaaagc cccatgataa tcacctgtaa agcgttacgt gttgactcaa
    47881 aaagtataga tctgcgggat gataattgcg gattgcagaa ataaaaaggg cgaagatgat
    47941 ctccgccctt ttcttatagc ttcttgccgg atgcggcgtt aacgtctgat ccggcctaca
    48001 aaatcatgca aattcaatat attgcacgag tctcgtaggc ctgataagcg tagcgcatca
    48061 ggcacgctcg cattgttaac gccattaaat atcaatattc gccgctttca gggcgttctc
    48121 ttcaataaac gcacggcgcg gttcaacggc gtcgcccatc agcgtggtga acaactggtc
    48181 ggcagcaatc gcatctttaa cggtaacgcg cagcatacga cggctttccg ggtccatagt
    48241 ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct ggatggagag
    48301 gccgcgacgg gactctttca ccagccagtc cagcgcttgc tcgaagctgg ctaccggctg
    48361 acgacgctcg ccacgttcga taaacgcatc ttcttccagc aagccgcgca gtttctcgcc
    48421 cagcgtgcag atacgacgat attcgccacc ggtgataaac tcgtgatcca gcggatagtc
    48481 agtatccaca ccgtgggtac gcacgcgaac aaccggctcg aacaggtttt gctcggcatt
    48541 agcgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca gttcgctgac
    48601 cagcgcgttc acccagcggg taacggtctg ctcatcagag aggtcagttt ccgtcagcgt
    48661 cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca tgcggttgat
    48721 cattttctgc gtcgcgttgt actcagacac cagtttctct aacgcttcgc cagccaatgc
    48781 cggtgcactg gcgttggtgt gcagcgttgc gccgtccagc gcgatagaga tctggtactg
    48841 atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca ctttgtacag
    48901 cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa
    48961 gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat
    49021 gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccaatac cacagccaag
    49081 cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc gcgctttctc
    49141 aacgttgagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg
    49201 cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcggaaa gcgccggatc
    49261 gcgttcctgg cagtctgcca gtttgcccgg caggcccgct aagtcgagcg cacctttacg
    49321 gcgggtcatt tcacgcgcgc gacgcgcagc ttcacgggca cgggcagcat cgataatttt
    49381 gccaaccacg attttcgcgt cggttgggtt ttccagcagg tattccgcca gcagttcgtt
    49441 catctgctgt tcaaccgccg atttcacctc agaagaaacc agtttgtctt tggtctggga
    49501 ggagaatttc gggtccggca ctttcacgga aacgaccgca atcaggcctt cacgcgcatc
    49561 gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca tgtaggcatt
    49621 cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg
    49681 aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact gcaacgccac
    49741 ttcgacgccg ataccatctt tttcggtgga gaagtagaag atattcgggt ggatcggcgt
    49801 tttgttcttg ttcagatatt caacgaacgc cttgatgccg ccttcatagt ggaagtggtc
    49861 ttctttaccg tcgcgcttgt cgcgcagacg aatggaaacg ccggagttga ggaacgacaa
    49921 ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg tgaaggtttc
    49981 gaggctgggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg taaccgccag
    50041 cggagcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct cgcgctggat
    50101 aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc cgtgcagacc
    50161 gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat
    50221 cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc cgcgcccatc
    50281 atcctgtacg gagacagagt tatcggcgtg aatggtgacg ataatttctt tacagtgacc
    50341 cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt gcagaccggt
    50401 gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat ccagcccttt
    50461 caggactttg atactggagg agtcataaga attcgacatc aacgtttctc gctcatttat
    50521 acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg
    50581 acatgtctat aacgtgttca gcactgattg cgctgacaaa gacctgtgat tgcgtcgctt
    50641 ttaagcggct ggcaagcaac ccgcgacgct catcatcaag ctcagaggca aaatcatcta
    50701 tcaggtagag acaccgccgc ccgctttcac gggtgaggaa ctctccttgc gccagacgta
    50761 aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc ggcgcaccgt
    50821 cggcgcgaat gcgtaagtcc gctttgtgcg ggccatgcgc ggtgtaggtt agctggcgat
    50881 cgcgttcaaa attacgttcc agcacctcag cgtactctgt ctctttctcc cagccgcgct
    50941 ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatca gccatatcgg
    51001 ccgcgatacc ggcgctatac tccgcgcgcc aggtactgat ttgctccgcc agcgggatca
    51061 gttctttatc ccacgggcgt agctgttcgt aacgtgtcac ctgacgcagc gccgcattgc
    51121 gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccgggt tcgttgtgaa
    51181 agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt aaagtaaacc
    51241 cttctggcgt tatcaactgc attggcatca ggtgcgccag ttccgcgacc ttatgcccgt
    51301 ctgtaccgtc gatgcggact ttgctgtcgc cctgtttgtc tttggttaag ccaatcgccg
    51361 tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa cgcctcctgc tcgtggcgaa
    51421 tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta tagatggctt
    51481 ccagcacgct ggttttgcca ctgccgttgg cacctaccag aaagttaaag ccgggagata
    51541 aagcgagatc cgcggtttcg atattgcgga aatcgcggat caacaagcgg gtgagggaca
    51601 ttacagtctc attggcatga caacataggc agccgactgt gatgcggcat cttcaatctg
    51661 cacgctggaa accgaatcgg tcagcatcat gcgcacgttt tcacatttca gcgcgttcag
    51721 aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc tataggtaac
    51781 gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcggtgattt tcagctggtt
    51841 ttcgctgaca tacagacgta cgccgcggaa tttctcgtta gagagaattg ccgcgcgagc
    51901 aaacgcctgc ttgaggagat cgcaacctgc ttccagatgt ttgtccgggt tcttcggcag
    51961 aacgcgtcgg taatccggga agcgaccatc caccagtttc gaggtgaaga taaagtcgcc
    52021 aacgtgggcg cgaatgttgt tgctgccaat ctgcacgcgc agcggattgt cgccgccgtc
    52081 gagcatacgc atcagttcaa tcacgccttt acgcggcacg atcaccgaat ggcttggcaa
    52141 agattgacca attggcattg aacagaccgc cagacggtgg ccgtcggtcg ccacggtgcg
    52201 cagttcttca ccttcggttt caaacagcat accgtttaag taatagcgaa cgtcctgatg
    52261 cgccatagaa aactgggtcg cttcaatcag acgcttcatc gtcgcctgcg gcagggtaaa
    52321 ttcgacttcg ctctgccagt catcgagatt cgggaaatct gccgctggca gggtagacag
    52381 cgaaaaacgg ctacgcccgg agcgcaccag catccgctca ccttccagtt gcacggcaat
    52441 ttctgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt
    52501 cgcccctggc tcgtgtggct gaaccagcgc aacgcgtgcc accatttcca tctcgagatc
    52561 agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac cgagaatcgg
    52621 tagcgtagga cgaccaccta acggcccgct cacctgttgt agcggtttta ataaatgctc
    52681 acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa ttggaaaaat
    52741 cttctttgat atcgtggctc tcttcacgca gctgctcgat cttccggcag gcatgaagca
    52801 ccgtcgtgtg gtcacgacca ccaaacgcat cgccaatctc cggcagactg tggttagtca
    52861 gctctttcgc cagcgccatc gccatctggc gcggacgcgc caccgagcgg gatcgacgct
    52921 tagaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc tgaatattgt
    52981 cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt
    53041 cgatggtgat cgcccgtccg gtaaagttgg cgttagcaat aacgcggttc agcgcgcctt
    53101 ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact tcgcccggca
    53161 aacgaatgtc gttttcgtcg gcctttttca tcaggatcgc cacgcgggtt tccagctctg
    53221 gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga tcctcaacgc
    53281 cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta ccttccagca
    53341 gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc tttattagca aaaaactgaa
    53401 tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct tcgatcgcgt
    53461 tgttttgcag ggcttttacc atgtcctgaa caaagcgctc ggagtgcata taaaccactt
    53521 tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg tgagttttac
    53581 ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg ttatctgcca
    53641 cctggcgagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag ttatcaaacg
    53701 tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccgga acgttatccc
    53761 aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg
    53821 ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt
    53881 caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca ttgatattat
    53941 taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac agggccagcg
    54001 tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct gtggctggta
    54061 actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg gcggactcca
    54121 ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga cgtacgtcga
    54181 aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga
    54241 tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga tcttctgttt
    54301 ctcacagatt tccccgattt atccacagga ctttccagaa ctcgctaagt gtaaacgatc
    54361 ctgccgcgag gcgggcacga tttacgccgc atattggaaa atttaatgac catagacaaa
    54421 aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac ccgtcagccc
    54481 gtataatcct ccacccggcg cgccatgctg gttttcactg gtgtgaggcc gtacattttc
    54541 cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact ccggagtgta
    54601 caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc gtttcatttt
    54661 tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa tcgccatgaa
    54721 acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc gtgctcgtat
    54781 ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct
    54841 gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt cccagggagt
    54901 tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa cgggctggca
    54961 cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt atcggtctta
    55021 cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa cgtctgacgc
    55081 gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg gtggtggcga
    55141 aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa aaattatggc
    55201 gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct atcaacgcct
    55261 gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa gctacggaat
    55321 tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga aacgcgtatt
    55381 aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac catttgatac
    55441 cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg
    55501 tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca ggcccaacag
    55561 accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt accggccagt
    55621 ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat caacacccgt
    55681 ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa ctctacccag
    55741 ccgttccagc tgctggaaac ttcaccgcag tttatttatc aggcacagag cggtctgacc
    55801 ggtcgtgatg gcccggataa tccggctaac ggcccgcgtc cgctgtataa cgttgaaaaa
    55861 gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac gtataccgac
    55921 gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac
    55981 gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg agatctccac ctttggtcag
    56041 ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa cttcgcactg
    56101 cacaccttcc gcggcgcggc gtactccacg cctgacgaga agtacgagaa atacaagttc
    56161 gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg ggtggcaatg
    56221 ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa caacttctat
    56281 accgctaatc tgggtaacgg catcgccgct atcggctata aatctcagcc ggtactggtt
    56341 cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga aatccaggac
    56401 aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg gttgtggttc
    56461 atctctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg taactggggc
    56521 ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct gaccaaagcg
    56581 cagtacacct ccatggcgaa gatgcgcatg ctgcagccga agattcaggc aatgcgtgag
    56641 cgtctgggcg atgacaaaca gcgtatcagt caggaaatga tggcgctgta caaagctgag
    56701 aaggttaacc cgctgggcgg ctgcttccca ctgctgatcc agatgccaat cttcctggcg
    56761 ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc
    56821 cacgacctgt ctgcacagga cccgtactac atcctgccga tcctgatggg cgtaacgatg
    56881 ttcttcattc agaagatgtc gccgaccacc gtgaccgacc cgatgcagca gaagatcatg
    56941 acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg tctggtgctg
    57001 tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta ccgtggtctg
    57061 gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga gttttcgcta
    57121 aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa gcaccgcgta
    57181 tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt agcccaggcc
    57241 acgcctccgg gacgtggcgg tgttggcatc ctgcgcatct ccggtctcaa agcccgtgaa
    57301 gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta tcttccgttt
    57361 aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc tggcccgaac
    57421 tcgttcaccg gcgaagatgt gcttgaactg cagggtcatg gcggtccggt gatcctcgac
    57481 ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc tggtgagttt
    57541 tccgaacgcg cgtttcttaa cgataaactc gacttagccc aggccgaggc gattgcggat
    57601 cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct gcaaggcgca
    57661 ttctccgcac gggtcaacca tctggtggaa gccctcaccc acttgcgcat ttacgtcgaa
    57721 gcggcaattg atttccctga tgaagagatc gatttcctct ccgacggcaa aattgaagcc
    57781 cagctcaatg acgtgattgc cgatcttgat gcagtgcgtg ctgaagcacg tcagggtagt
    57841 ttgttgcgcg aagggatgaa ggtggtgatt gccggacgtc ctaacgccgg taaatcgagc
    57901 ctgttaaacg cgctggcggg ccgtgaagcg gcaatcgtga ctgatatcgc cggaaccacg
    57961 cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat catcgacact
    58021 gccgggctac gtgaagccag tgatgaagtg gaacgtattg gtatcgagcg cgcgtggcag
    58081 gaaattgaac aggccgaccg cgtactgttt atggtcgatg gcaccacgac agacgccgtt
    58141 gatccggcag agatctggcc ggaatttatc gcccgtctgc ctgccaaact gccgatcacc
    58201 gtggtacgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga agtgaacggt
    58261 catgcgttga ttcgtctctc ggcaaggact ggcgaaggcg tggacgtgct gcgtaaccat
    58321 ctcaaacaga gcatgggctt tgacaccaat atggaaggcg gcttcctggc gcgccgtcgc
    58381 cacttacagg cgctggaaca agcggcggaa catctacaac agggcaaagc gcaactgttg
    58441 ggtgcctggg caggcgaact gctggcggaa gagttgcgcc tggcgcagca gaacttaagc
    58501 gaaatcaccg gggaatttac ctctgacgac ctgctggggc ggattttctc cagcttctgt
    58561 attggtaagt aagtcgtcta aattgatata aagggcactg ttccgttctg ggggaaacta
    58621 actatcagaa cggaacagcg cttgtttttt atgagcatcc tgcaccaaaa ctgcatttgc
    58681 tactggctgg agcgacttta aacaccttgc agtcctgtct ttacagagaa cgatattgct
    58741 ttaacctatt aagctcctct tgcggaaagt taaattcgtc tactgctttc cagaatagtt
    58801 ctttaagtac aggattgcca tttgccagac ttattacaga tttaccttca ttattctttt
    58861 cttccagctg catcatcaca tctttaaggt aattttcatt ttcaccaacc acaattttga
    58921 atattttcag ccagagcccc atatgtatat catccccgtt atttaccaca tgagagaatg
    58981 acttgccatc aaagtcacgg gtaaactgct ccacaatttc atcgaaagag aacccgtgtt
    59041 gcaacgccag gcgaatcgcc gcattcagat atacgtcaat gacctcttta ctcattttct
    59101 taaagcttgc aatatgcaaa gagcaggcag cattatttct gctcaacgac agtagttctc
    59161 tcactacatc tttatcattt tcataatggc tggctatcac ttcagctgtc tccatccagg
    59221 cagcaacaat ctccgtatgc cctttcacta tcgcagaata taagcctgat gccttcgtcc
    59281 atttgtctga ctcccccgtc cattctcttg ctgccatcag tttcaatata tcttctttat
    59341 ttatttcatt tttaaacatt ttggctttta tattggctag caatatatta acaacattat
    59401 aattattggt cctaatagcg ctatttaaat cataaattga gtctatttct tttcctacac
    59461 tacgaagtat ttcattttta tttactccaa actctttctc aaagtcatct atcgccaaca
    59521 accattcttt gacaatttta ttttgattat ttcttaaagc cttaggaatc cagctcacac
    59581 cgaggtaatc tttcgcaagg agtatatttt ccagatcgct atggctaaaa ttatactttc
    59641 tggtcaactc ttttaaattt attatgaatt cattaaaaac atcaatacta ttttgatacc
    59701 ataatacata taatccatat gttcctgtaa ttgatttcgc agcaagaacc tttgatgctg
    59761 tcatttcttg ggagttttta aatttatcaa aaaaaagaac aatgttttca tgcatcccca
    59821 tttgtattaa tagatgtaat gctaatggtg ataatggtgg ttgcaactca ttatttatac
    59881 cacttaataa atgggcttgt tctcgaggga gataaggagt tattcctaca cagtcggagc
    59941 aaatttctgt cattacccat tttttttgtg cctgtttaat attcataaag gatcgcaatg
    60001 atccaaaaga atcacagttg tttgctttat agcgtactgc agtattggtt gcattaggat
    60061 cataaaagga tacaacatat tctgtttctc cagcaggcgt ttttttgatc ctcaagcgga
    60121 cggccatagc gtgagtttga gattgaacga ttgccgccat atgcgttaac ttctcttttt
    60181 ccattctgtg aaaaaagact gataatgcct ccccaaactt atcacaggca acaacgcatg
    60241 taccgggtgg tggatttttt tgtagattgt caaattgctg ctcaagctcg aatggaatat
    60301 gtttttgtat atcttccgga gatgaatagt aagcctttaa tggaactttc cctgtatgtt
    60361 cattcattga atctatacaa tattgtagag aaagatgcct gcacacgatt tcattttctg
    60421 gtttttcatt aaaaaaaatc ttacagttgt agtttataac gtcatatttg ctattagctt
    60481 ttatctgctc ttttgtaaaa ctctcatcat cagggtacgt atgaaggcaa gtaaaaatat
    60541 catccaggaa ttttataagc gctggagata cggaaatatg tactatattg attgcgtctg
    60601 catcgcttac ggttaaaatc ataccttcag gttgttgctt agatctggag acaagaattg
    60661 aatatgcacc atcgctatag aaacctaact caacttcaga acaataatta acaaaatgat
    60721 ttaacggccc ctctttttca agtgaataaa ctgaaggatt tttaagatac gccactatat
    60781 cgtcaacgat caattgagtt ttattttttc ctccatccaa atcaggcatg aaagaaggag
    60841 attgtgcaca tacactgcca acagttacgt tatcaaccat tttctttccc ctcatccata
    60901 tgaaagttgt agtaataata aggttactat ttaaaaacca actatcaaat ttcgctcatt
    60961 tatagagtta tttgtctttt tacgtcatta ccccatcagg tcatcaacct ggtaatgaag
    61021 tgagtaaacc gcgacgtcaa tgagggaaac agctccttgt aatggaaccc ctataatcaa
    61081 tgccatcatc ttcaacaata atgataaaaa ttaaaaaatt tatccattta tggatttttg
    61141 ctaatatttt gcatgggcca gccatcacaa acacagacaa tatctaattg tgattcgatt
    61201 cacatttaaa caattttaga atagacaaaa ttcttgagta ttatactgta gcctcgtgtc
    61261 tttcgaggat aagtgcatta tgaatatctt acatatatgt gtgacctcaa aatggttcaa
    61321 tattgacaac aagattgtcg atcatcgccc ttgatttgcc cttctgtagc catcaccaga
    61381 gccaaaccga ttagattcaa tgtgatctat ttgtttgcta tatcttaatt ttgccttttg
    61441 caaaggccat ctctcgttta tttacttgtt ttagtaaatg atggtactta catatatatc
    61501 tggcgaatta atcggtatag cagatgtaat attcacaggg atcactgtaa ttaaaataaa
    61561 tgaaggatta tgtaatggaa aactttaaac atctccctga accgttccgc attcgtgtta
    61621 ttgagccagt aaaacgtacc actcgcgctt atcgtgaaga ggcaattatt aaatccggta
    61681 tgaacccgtt cctgctggat agcgaagatg tgtttatcga tttactgacc gacagcggca
    61741 ccggggcggt gacgcagagc atgcaggctg cgatgatgcg cggcgacgaa gcctacagcg
    61801 gcagccgcag ctactatgcg ttagccgagt cagtgaaaaa tatctttggt tatcaataca
    61861 ccattccaac tcaccagggc cgtggcgcag agcaaatcta tattccggta ctgattaaaa
    61921 aacgcgagca ggaaaaaggc ctggatcgca gcaaaatggt ggcattctct aactatttct
    61981 ttgataccac gcagggccat agccagatta acggctgtac cgtgcgtaac gtctatatca
    62041 aagaagcctt cgatactggc gtgcgttacg actttaaagg caattttgac cttgagggat
    62101 tagaacgagg cattgaagaa gttggcccga ataacgtgcc gtatatcgtt gcaaccatca
    62161 ccagtaactc tgcaggcggt cagccggttt cactggcaaa cttaaaagcg atgtacagca
    62221 tcgcgaagaa atacgatatt ccggtggtaa tggactccgc acgctttgct gaaaacgcct
    62281 atttcatcaa gcagcgtgaa gcagaataca aagactggac catcgagcag atcacccgcg
    62341 aaacctacaa atatgccgat atgctggcga tgtccgccaa gaaagatgcg atggtgccga
    62401 tgggcggctt gttgtgcatg aaagacgaca gcttctttga tgtgtacacc gagtgcagaa
    62461 ccctttgcgt ggtgcaggaa ggcttcccga catatggtgg tctggaaggc ggcgcgatgg
    62521 agcgtctggc ggtaggtctg tatgacggca tgaatctgga ctggctggct tatcgtatcg
    62581 cgcaggtgca gtatctggtc gatggtctgg aagagattgg cgttgtctgc cagcaggcgg
    62641 gcggtcacgc ggcattcgtt gatgccggta aactgctgcc gcatatcccg gcagaccagt
    62701 tcccggcaca ggcgctggcg tgcgagctgt ataaagtcgc cggtatccgt gcggtagaaa
    62761 ttggttcttt cctgttaggc cgcgatccga aaaccggtaa acaactgcca tgcccagctg
    62821 aactgctgcg tttaaccatt ccgcgtgcaa catatactca aacacatatg gacttcatta
    62881 ttgaagcctt taaacatgtg aaagagaacg cggcgaatat taaagggtta acctttacct
    62941 acgaaccgaa agtattgcgt cacttcactg caaaactgaa agaagtttaa ttaaaacatt
    63001 cagagtggct ataaggatgt tagccactct cttaccctac atcctcaata acaaaaatag
    63061 ccttcctcta aaggtggcat catgactgat caagctgaaa aaaaacactc tgcattttgg
    63121 ggtgttatgg ttatagcagg tacagtaatt ggtggaggta tgtttgcttt acctgttgat
    63181 cttgccggtg cctggttttt ctggggtgcc tttatcctta tcattgcctg gttttcaatg
    63241 cttcattccg ggttattgtt attagaagca aatttaaatt atcccgtcgg ctccagtttt
    63301 aacactatca ccaaagattt aatcggtaac acctggaaca ttatcagcgg tattaccgtt
    63361 gccttcgttc tctatatcct cacttatgcc tatatctctg ctaatggtgc gatcattagt
    63421 gaaacgatat caatgaattt gggttatcac gctaatccac gtattgtcgg gatctgcaca
    63481 gccattttcg ttgccagcgt attgtggata agctcgttag ccgccagtcg tattacctcg
    63541 ttgttcctcg ggttgaagat tatctccttt gtgatcgtgt ttggttcttt cttcttccag
    63601 gtcgattatt ccattctgcg cgatgccacc agcaccactg cgggtacatc ttatttcccg
    63661 tatatcttta tggctttgcc ggtgtgtctg gcgtcatttg gtttccacgg caatattccc
    63721 agcctgatta tttgctatgg caaacgcaaa gataagctaa tcaaaagcgt ggtcttcggt
    63781 tcgctgctgg cgctggtgat ttatctcttc tggctctatt gcacgatggg gaatatccca
    63841 cgagaaagct ttaaggcgat tatctcttca ggcggcaacg ttgattcgct ggtgaaatcg
    63901 ttcctcggca ccaaacagca tgggattatc gagttttgcc tgctggtgtt ctctaactta
    63961 gctgttgcca gttcgttttt tggtgtcacg ctgggattgt tcgattatct ggcggatctg
    64021 tttaagattg ataactccca cgccgggcgt ttcaaaaccg tgctgttaac cttcctgcca
    64081 cctgcgttgt tgtatctgat cttcccgaac ggctttattt acgggatcgg cggtgccgga
    64141 ctgtgcgcca ccatctgggc ggtaataatt cctgctgtac tggcaatcaa agcccgcaag
    64201 aagtttccca atcagatgtt cacggtctgg ggcggcaatc ttattccggc gattgtcatt
    64261 ctctttggta taaccgtaat tttatgctgg ttcggtaacg tctttaacgt gttacctaaa
    64321 tttggctaaa tcatccttca agaagccagc catttcgctg gcttcttgcc tgtcaggaaa
    64381 tcacttatgt ccaaatggca actcgcctga ttctccttca ccacgtatgc tttgcgtcac
    64441 cttactatca ggacgcttta gcccatgtcc cgctttttga tttgtagttt tgccctggtt
    64501 ttactgtatc ccgccgggat tgatatgtac ctcgttggtt tgccgcgcat cgccgccgat
    64561 ctcaatgcca gcgaagcgca gttgcatatt gcgttctccg tttatctggc gggcatggca
    64621 gctgcgatgt tatttgccgg taaagtggcc gatcgttcag ggagaaagcc tgtcgccata
    64681 cccggcgcgg cgctatttat tatcgcctcg gtgttctgtt cactggctga aaccagcacg
    64741 ttatttcttg cagggcgatt tctacagggg ctgggcgcgg gctgttgtta cgtggtggcg
    64801 ttcgccattt tacgcgacac gctggatgat cgacgtcggg ctaaagtgct gtcgttactc
    64861 aacggcatta cctgcatcat tccggtatta gcgccggtgc tcggacacct gattatgctt
    64921 aaattcccgt ggcagagtct gttctggacg atggcaacga tgggcatcgc ggtactgatg
    64981 ttgtctttgt ttattttaaa agaaacgcgc cctgcggccc tcgcagcttc tgacaaacca
    65041 cgagaaaata gcgagtcgct gcttaaccgg tttttcctca gccgtgttgt tatcaccacc
    65101 ctcagcgttt cggtgatcct caccttcgtc aacacgtcgc cggtattgct gatggaaatc
    65161 atgggttttg agcgcgggga atacgccacc attatggcgt tgaccgctgg cgtcagcatg
    65221 accgtttcat tctccacacc atttgcgctg ggaattttta agccacgtac gttgatgatc
    65281 acctcacagg tgttattcct ggcggcgggg atcactcttg ccgtttcacc ttcccatgcg
    65341 gtttctctgt ttggtatcac gctgatttgc gccggtttct cggtaggttt tggcgtagcg
    65401 atgagtcagg cgttagggcc gttttcatta cgcgcgggcg tagccagctc gaccttaggt
    65461 attgcgcagg tttgcggttc gtcactgtgg atttggctgg cagcggtggt tggtatcggc
    65521 gcatggaata tgctgatcgg gattctgatt gcctgtagca tagtgagcct gttgctgatt
    65581 atgttcgtcg cgcctggacg ccccgttgcc gctcatgaag aaatccatca ccacgcttga
    65641 tctcaatttg ctgctctgtc tgcaactgct gatgcaggag cgcagcgtga ccaaagcggc
    65701 gaagcggatg aacgtgacac cttcggcggt gagtaagtcg ctggcaaagt taagagcgtg
    65761 gtttgacgac ccactctttg tgaactcacc gctgggcctg tcgcccacac cgctaatggt
    65821 cagcatggag caaaatctgg cggaatggat gcaaatgagc aacctgctgc tggataaacc
    65881 gcatcaccag acaccgcgcg gcctgaagtt tgagctggcg gcggaatcgc cgctgatgat
    65941 gatcatgctt aatgcgctgt cgaaacggat ctaccaacgt tacccgcagg cgaccatcaa
    66001 attacgtaac tgggattacg attccttaga tgccattact cgtggtgaag tggatattgg
    66061 tttttctggt cgcgaaagtc atccccgttc gcgggagctg ttaagctcgc taccgttagc
    66121 cattgattat gaagtgctgt ttagtgatgt gccctgcgtc tggttacgca aagatcatcc
    66181 ggcactgcat gaagcatgga atctggacac ctttttacgt tatccgcata tcagcatttg
    66241 ctgggaacag agcgatacct gggcgctgga caatgtgtta caggagctgg ggcgcgaacg
    66301 cacgattgct atgagcctgc cggaattcga gcagtcactg tttatggcag cacagcccga
    66361 caatctgcta ctggcgaccg cgccgcgcta ctgtcagtac tacaatcaac tccaccaact
    66421 gccgttggtt gctcttcctc tcccgtttga cgaaagccag caaaaaaagc tggaagtccc
    66481 ttttaccctg ctgtggcata aacggaacag ccataatccg aagatcgtct ggttacggga
    66541 aaccattaaa aatctttacg cgtcgatggc ataaccgaat cgtatgaaac gcgaccctat
    66601 ttcacaataa aatgtaaaaa agttgtaata aacttatctg aatcgaactt ttagccgctt
    66661 tagtctgtcc atcattccag taaatgatta ctcttgtatt cataatggac cattaagcat
    66721 ggagcgaaaa atggcgactc actttgcccg agggatttta acggaaggac atctgatttc
    66781 tgttcgtctc ccctcccagt gtcatcaaga agcccgaaac attccccctc atcgtcaaag
    66841 ccgttttctg gcgtccagag gtttactcgc agaactgatg ttcatgctgt atggcattgg
    66901 cgaattgccg gaaatcgtca ccctgccgaa aggtaaaccg gttttcagcg ataaaaattt
    66961 gccttcgttt tctatttcct atgccgggaa tatggttggc gtggcgttaa caaccgaagg
    67021 tgaatgtggc ctcgatatgg aactgcagcg tgcgacgcgc gggtttcata gcccacacgc
    67081 gctcgataac cacacctttt ccagcaatga atcgctatgg atcagtaaac aaaacgatcc
    67141 aaacgaagcg cgggcgcagc tcatcacgct gcgccgaagc gtgctaaaac taaccggtga
    67201 tgttttgaat gacgatccgc gcgatctgca gctgctgccc attgccggac gcctgaaatg
    67261 tgctcatgta aatcatgtag aagcgttatg cgacgcggag gacgtgctgg tgtggtccgt
    67321 ggcagtcacg cccacgattg aaaagctcag tgtctgggag ttagatggca aacacggctg
    67381 gaaaagcctg ccggatattc acagccgcgc caacaatcct accagccgga tgatgcgttt
    67441 tgcccagctc tctaccgtga agtctttttc gccaaattga tagacaacca caggagtcat
    67501 catgtctgaa aaattgaagg tggttacgtt actgggaagc ctgcgcaaag gctcatttaa
    67561 tggcatggtt gcacgtaccc tgccgaaaat tgctccggcg agcatggaag tcaatgcgtt
    67621 accttccatt gccgacattc ccttgtatga cgctgacgta cagcaggaag aaggttttcc
    67681 agcaacggtt gaagctctgg cggaacagat ccgtcaggct gacggtgtgg tgatcgtcac
    67741 gccggaatat aactactcgg taccgggtgg gctgaaaaat gccatcgact ggctttcccg
    67801 cctgccggat caaccgctgg ccggtaaacc ggtgttgatt cagaccagct caatgggcgt
    67861 gattggcggc gcgcgctgtc agtatcacct gcgccagatt ctggttttcc tcgatgcaat
    67921 ggtgatgaac aagccggaat ttatgggcgg cgtgattcag aacaaagttg atccgcaaac
    67981 cggagaagtg attgatcagg gtacgctgga ccacctgacc gggcaattga ctgcatttgg
    68041 tgagtttatt cagcgagtta agagctaaat aaaaaacccg ccagcaaaca tgcatggcgg
    68101 gtttttaacg cgctatcgat tttagtgagc gtcgataaac acaatcttca ggataaacag
    68161 cagcgcaacg atgattacgc acgggcttag atcacgcaga cgtccggtac caatcttcat
    68221 cacgcagtag gagataaagc ccagcgcaat accttcggta atcgagaagc tgaacggcat
    68281 catcacagcg gtaataaacg ccggaacaca ttcagtaaga tcctgccagt tcacgcgagc
    68341 aagacttgag gtcatcagta cgcccacgta gatcagcgcg ccagctgcgg catatcccgg
    68401 caccatcccc gccagcggcg acaggaagat aaccagcagg aacagcagac caacaacgac
    68461 tgccgtcagg ccggtacgcc ccccaacgga aacgccggaa gaagactcga tatacgccgt
    68521 aacagaagaa gtcccgataa acgaaccggt cacggaagag atgctgtcga catacagtgc
    68581 ctgcttcatg cgcgggaatt tacctttttc atccgccagt cctgctttat ctgtcacgcc
    68641 aatcagcgta ccggaggagt caaacaagtt gaccagcatg aaagagaaaa tcacccctgc
    68701 cagcccaagg ttaaacgacc cggctaaatc tacatgaccc acgacagtca ttacgctcgg
    68761 cggcgcagaa acgatgccat tgtagtggac atcacccagc atccagccca gcagcgtcgt
    68821 caccacgatg gaaaccagca ccgccgcgtg aatgttgcgt gaggccagaa tagcaatgat
    68881 gaagaagccg aggatcccca gcagtacgct gtgagaagtc agattaccga tgctcaccag
    68941 cgtttccggg ttagcgacaa tcacacctgc attcttcagt cccatcatgc caataaacag
    69001 gccgataccg ctggtaatgc ccacacgcag actcaccgga atgttggcta tcatccagta
    69061 gcgaacgcgg aaaatcgtca gtaacagcag accaatcgcg ccccagaaga ttgcgcccat
    69121 cccgacctgc cacggcaagc ccattgcctg cacaaccaca aaagcgaaga acgcattcag
    69181 gcccatagcg ggtgccagtg caactggcag gttagcaaac agtcccatca taatactgcc
    69241 gaatgcagca atcagacagg tcgtgacgaa gacggcgctg gtatccatgc cagcaacgcc
    69301 aagaatttgc gggttaacaa aaacgatgta aaccatcgtc aggaaggtgg taaaaccggc
    69361 gatcacttcg gtccgtgccg tcgtgccatg ttcgcgcagt ttaaacacgc gttccagcat
    69421 cccctggcca gaagcctggg ttgtgtgttg ttgactcatt atctatttcc gaacaaggag
    69481 ggaaaatccg tcgctatcgt ataccaaaat gagacaatat ggacggctga agataacttt
    69541 ttttcattgt atttgcttat acggcaacga ttgcgtcatg caaattggca tgacgaaaac
    69601 gttaaactaa cgataaagga aagacatgtc ccagatagaa gcggtatttt tcgactgcga
    69661 cggtacgctg gtcgatagcg aggtcatttg ctctcgcgcc tatgtcacga tgttcaggga
    69721 atttggcatt cacgttgagc tggaagagat ttttacccgt tttaaaggcg taaaactcta
    69781 cgaaatcatc gacatcatca gtaaagaaca gggcgtgacg atggtccagg ccgatgccga
    69841 acacgtttac cgcgcagaag tagcacgtct gtttgatacc gaacttgagg acatcgcagg
    69901 agcgaacgaa ttgctggcaa gcattaacgt gccgatgtgc gtcgtctcta acggtccggt
    69961 cagcaaaatg cagcattcgc ttggtaagct caacatgctg cactacttcc cggaaaaact
    70021 gttcagcggt tacgacattc agcgttggaa gcccgatccg gcattaatgt tccatgcggc
    70081 aaaagcgatg aatgtgaacg ttgaaaactg cattctggtc gatgactcca gcgccggcgc
    70141 gcaatcaggg atagatgccg gaatggaagt gttttacttc tgcgctgacc cgcacaataa
    70201 accgatcgtt cacccgaaag tcaccacctt taccgagctg gcgcagctgc ctgcactctg
    70261 gaaggcacgc ggctggaaga taacgcgata atcgcctcct tacttcaacg cttacacatc
    70321 agtcacgctg gtttgagata aaaaatacca ggtgtctgat gagtaagcgt ctattcccta
    70381 taaataattt atgtcgttaa tcgtccagtc gctcaccctc tgtatggtta agcctggttt
    70441 atatttttca caatatcggg ataccgttcg aaccaatcca gaatattaag catcgcgtta
    70501 ctgatatttt tattcgcttt atattgattc tcgcgtctat gatttttaac aatattaagg
    70561 ttagaacgta tcttattgtc atcgtcagca atcttctgat ttaatgccgc tacttcatcc
    70621 tgtacttctt gactattcca cttgtccctt ttgttatcaa ataaagtcct tttatcatct
    70681 actgatttat cacgctttat ggttaactct ttaatttctc gcaataagtc atcgtaatct
    70741 ttaatggaag ctcttatttc ctgatagttt tttagtaaat cctccctgtt aattgcgaat
    70801 acactggaaa aatagtgaaa atcgcgagca tatttattga gccttgaatc gtaatctgtc
    70861 ttaaaagatt ttatatgttc cagttggttt gatagtgctt taatttgttt ttcttcaaca
    70921 ccttcaaaaa tgggtgcaag ttccttatat tcaaatgtta catttctaac agattcttta
    70981 tgagactcct taattttctt ttggataaat tcggttggat tttcagtaag tattgttcca
    71041 tctggcggcg gaatcatttt atttattata gattcaacaa acttaattcg ttcattttct
    71101 atcatattgc taatgatatt ttgtgctttt acctgcatat ctttgaaaat aaaaaactca
    71161 atcctactat tatcaaaatg atattttccc tcgtacgatt tattttcaac ataaagtgtt
    71221 gataaaaacg actctaactc taaaaatgcg tttttatatt tttccttagt ctttgactcg
    71281 gaattataaa aatttattac atcgatattg atagcactta ccttttttaa gaaatcagat
    71341 atttgttctt tacttatagc tccgtctaga tccatataca tgtatttttg cgctgtaaga
    71401 ccatcttttg aataatttgt atttggatca atagcacagt ctttgcacct atctccactg
    71461 taataatagc ctaaatttgc cccaatcaaa ttactatttt taaagaagac attattgttt
    71521 tctgtatttt tcttgctaaa tgaagaatcg gtccctttct ttatactctc tacgcatttc
    71581 gaaaaattat catctaactt aagatttctc gatcttcttt caatgtaatc agaaaaatac
    71641 tctttggggt taaacataga tctatctgaa ccatcttcac gaagaccatc gtttatttta
    71701 atatgcttca aattacaatt ataaaacaca gtccccgtat ccggacccct gatttcatca
    71761 taattagact tgttgaacga atttgccaaa aaatccattc catgagcatc aagccctatt
    71821 atattacagt tataaaaatg actcagaagt atactcgagc caggacaaca tcctccctgg
    71881 attatacaat cctgaacgaa agactgcata aagagataag aacctaattt ttcttgaata
    71941 ctatcaggtt gtgtaacggt gtagatttta gacgtctcac atctataatt ttcaattttg
    72001 catttagaaa taaaacaacc tttaaacata ccttttattg gttttttatt tcctgacccc
    72061 acagtatcat cattagtctc ataaagctgt atgttgttga atttacaatc ctcgaaataa
    72121 caattttcaa aaaccggacc ttcaaggtta gggctgttta tcactcctga aaaaactaca
    72181 tttttaaaac tacaatattt taatgaattt aaaacatcct tagacaacga tgataaattg
    72241 attgttgtat taataatagg aattttattt ttaatcagtg tcgtaagttt tacagcataa
    72301 tcatcaaaca taaatctctt caagggagaa ttatcaaaat gtaaatcatc aatataatta
    72361 tcaagcgtta atctctctga agaaacctgt tttttttcgt tatcattcga agtagccccc
    72421 gtgtagagat taaaggatct actcacaata acttcattat tttcatttgt ggtaaagtct
    72481 gtcttagtac taaatcgcca ttgttgttct ttttttgcca gagatttaat cttatcgaac
    72541 acctcggctt tgttatcaac tttcgtgaca aattcgtaaa ttaagccttt gacttcttta
    72601 atatgagagc cgggaaaaag atcgcgaaca aactcccaca gtgtacgttt tttaaataaa
    72661 tcccctttgc catcaagaat attttcaata tccttagagt ttacattata tttcttatta
    72721 ttaatactta cagcaatttt atcacaatat cctttatgat taagtctctc cttattcatt
    72781 gcttctaaaa aaggattagt attaaaaaga acatctggtt ttaatttagc aaccataaac
    72841 cctccggcgt gcaatattga gttgttttgt ttgaaaaaca ataacttcta tgttgttaat
    72901 taactgtact gctagttagg ttagcaacaa gtgttactta tattaccggt gaatactgta
    72961 aaaaatagct cattatttcc attttaaccc agggtgttca ccctggattt gcaattttta
    73021 gggggttaac ggtacattcc acgggttaac cgcagcatca gaaaatgagc gtttttttat
    73081 agccgctttt attgtcgagg ttaaaataaa tagatttcat taacttttat caaggtgatt
    73141 tatggataat cttattggca caccaccaaa ccacgcagtt ccccataagt atatggatat
    73201 ggagcaaatg gaatatttat gtcatctcaa tcgtttttct aaattgtctg acgatttttt
    73261 aataaatggt cagagagagg acctttttac atacaaatat gttttagatg gctctttttc
    73321 aaatttacag catttacttc ccaaaggaca actacagaag atacaacagc gattaagttc
    73381 tcttatgtat aaaaacaagt ttcattgttt tcatgttttt ttattaaaac tatgctcaat
    73441 tgagagtatt cctctcccaa atgccgatta cgctttcttt gatgacgaaa tgccatttac
    73501 attaactgat gaacagatcg aaaatatttc ttttttaaac gcatatcaca aagaaaagaa
    73561 aggaaacaac gacattgtta catttgattt catgagcgct gatcaccacc ataattttag
    73621 cacaaccata gctttaacaa atgatagctt ccatatatct tcaataaaca accataacgc
    73681 acaggttata ttcgatgaaa acatccattt acatccctat gaattaccag aatcatcaca
    73741 atggtgttat cagttaataa aaaacatgat ttctttacat tgcagatata ataacaattt
    73801 caaaataaaa aaaacctgtt gttttgattt taaaaataaa tctttttgta atgccaggtt
    73861 gcaatatata acgtttattg catgtttatt ttcacatgca aacatgaaat attcgacatt
    73921 tcatgatgtt aaccttgata tgtgtgagat aaagaattgc aattttgaca attcagagat
    73981 gaatttcatt tcgtgcgtcg ggacaaattt tagcggttct acatttaaca atgtaaaaac
    74041 aacaacagcg caactaatta agacaccaac aaagtggacg aataatactc taaaatattg
    74101 gttctccagt tgtaataaac gtaatatcat cttcactttt aatacaatat ctgatagaaa
    74161 tatgaaatta aaaggtatca aggatatatt actgtcgttg gttgaccaaa aagtcaatat
    74221 ttatagtgta aggcaagagc ttctggattt tcttaataat gatttatata aaaataatgg
    74281 agagattctc tcttataaag aatcaataat gttgttttgt gctgtgtagc cgaaaaatag
    74341 atgattcaga ttaactaccg cactctatgt tgtgtttata tccatgtcag gtaacaggtc
    74401 atttgtgaat cctgacacca gactatgact atcgcccagg gataatccac aaccgctgga
    74461 agtaatctca tttctgcagg ataaaaccgt agagtgcgat tgcgatgaat aataaataca
    74521 ggcttattat tccagtcttt tttattgcgt tggttgtttc aaatggctcg gttagtaaaa
    74581 tgcaccattc gctcggaaaa ctcaacatgt tgcactctta tacgccgtct ggaaaaggcg
    74641 taggagtggt ataaccattt aatcgcctcc caacgtcaac gcttacacat cagcgacact
    74701 ggtttaatta aagcatacca aatttctgat gagtaagcgt cttttcgtat aacctcattg
    74761 tcacaggaac gacttgatcc gtttatttat tcgccatgtt attcagtatc aattttctta
    74821 atagctttca ccatattttc attttctttt agccaactcc tgatattttg aactacatca
    74881 ttaagtgtat tttcttgttg atactctttc tccaatctct ctgtttcatc cttaagatgt
    74941 agaatctctt tacttatatt tggtattttt ccatctatca cctttattat ccccttaatc
    75001 tcattaattc tttccgcatc ttttttatct ttattgtctt tattatttaa gtaaaaaccc
    75061 tctttaatca attgatcctt agtatcgact aaatcatttc tttcgacaat aagtttggca
    75121 tatttgtctt ttatatcgcc ataattttcc ttattatagt cattattttt ctcaattacc
    75181 caccgtaaat attgctcaat gctagagaat aaatcgactt tatcatctac atattttgat
    75241 actttcctat aatccctgta atttatttcc gttctttcct taaattcatt caatgctaaa
    75301 aaaatctttt gcattttatc aaaattatca tcttcatgat tattattata gtctttgtaa
    75361 atatcccgca aatcattaaa attaaagtgg aagtgaaatc ttgcgctgtt tgaaatagca
    75421 gaacgattta tttttaaata attacgttca tcgtaatgtc tttgccaaaa gaatccagta
    75481 tagaggttat gcatgaaatt cactttatca atatctattt cattggctat atctttttct
    75541 atattgtaca aaacatttaa tataatatac tggatagctt caccattaag ttgatatttt
    75601 ttgccttcat catcaagatt gaagctaccc agcttgacca acaaagattg taaatataat
    75661 agttcctttt cgtaatgact tatcgcatcc ttgccataat tattgaattc ttctacccat
    75721 gaattaactt ccattaaatt ttccatgaaa tctgaaatgc cattttcagc tatagctcct
    75781 ttcatgtcca tataaacagg aattctaatc tggctattcg cagcaagatt accacttaac
    75841 caagtggttg aaggatcaat agcacaatca tccaatttaa ttccattgat tgactcgccc
    75901 aaacaggttt taagtaaatt gcaattacta agttttaaat atttatctat atcatcagca
    75961 acgtatttat catttatata acgaccgcca ctcatattag ggaaaagatt aacgtagata
    76021 aggtttgaaa tgtgtttatc cagattaagt ttacctccat cctttttgaa atattctctg
    76081 ggatcaaaat ccagtaaatc tttgcgaatt ccatttcgtt tgcatgtcat atcagaggag
    76141 ctaaaatcac acatataaaa atgattatca tattttaatt cacatctata tttgtgataa
    76201 aacgaagtcc ctatgaaatc catttcagca ccattaagct ttgctatttt acaacataac
    76261 aaactgcttg caataatact tgcattttta cacactccat tcagaagagt acattcatat
    76321 acaaatgatt gcataaacag atatgcacca actggcttac ccacattggg atcttgattt
    76381 acagagtata tttttgatgt atctatgtta aaatttttta tttcacatcg atacagaaaa
    76441 cactctttaa acacgccgta aataggtatt tcattattta gttttactcg acacctggag
    76501 cttataagtg acacattctc aaatttacag ccctcaaaaa aacaattttc aaatactgaa
    76561 ccattaagat tttcgtaaga tatattacca gtgaatgtca catttttaaa gttacaatat
    76621 tttaaagatg ccagggcttc tttttccagt gcagataaat caattgtgat attttcaaag
    76681 gggattttat tttttaaata tacgctaagc ttttcattat catttgggaa ctgtattttc
    76741 aacatccctg attgaaaagc tttctcatca aaatgaatat catgcttata aatatcaaaa
    76801 ctgatttttt ctgaaaataa agaatgcatt gtcttttttt cagcatcaat atgatgatct
    76861 tgcgcattaa tatttatttt aaatactcta ctaacaataa tatctttatt ttcatctaca
    76921 ataaattctg tcgtcgcatt aaagcgccat tgttgatctt gatcggcgag agatttaatt
    76981 ttattaaata tatcagcttt atcaccagtt tccgacacga aatctataat ggcttgttta
    77041 gcctccttaa tatgatacat gggaaaaaga tcgagtataa attcccaaag acttcgttta
    77101 agaaaagatt gactatcacc agctaaaatg tttgttatat ctttatgagt tactgaatac
    77161 ttacccacat catcaacaga caaaccaatt tttcctgaat atttttgcgt tctatttttt
    77221 tcattcagat tttcatctgg cattttagtc agagtagcaa atttatctgt aactgtacta
    77281 tatgatgata tagccataat catttctcct gaacacaata tttattcgcc cgagcacaac
    77341 acattgccga tttgtcttaa aaaagcatac ttgcgtgttg gtttgctacg attatgacta
    77401 aaccacctat gaatactgta aaaaatcgct cattttttat gtttcgatat agggctttta
    77461 ccctggtttt tgttttcttt tccattctct gtgcaggcag cgatataaat gaaaggaata
    77521 ctaaaaatgt ttttatagcg gggcactaca ttaaaagtaa aataattcaa atgaaataat
    77581 gtatatgcaa tgtactggga gagtttcgag gtgagtttgt gtggcaatat ttcagggaga
    77641 aagtttatca gcccggtaac tatgtatgac cgggctgatt gccagattcg gcgtaaaagc
    77701 cttatctgac ctacagttag cgttactctt tcgggtcttt tccggccagc agtttatcca
    77761 gctcatcacc gcctacatga cggaaatcct gacctttcac gtagtagaag ataaactcac
    77821 agatattctg gcagcggtcg ccgatacgtt cgattgaacg cgcacaaaac agcgcggtaa
    77881 gtacgcttgg aatggtgcgc gaatcttcca tcatgtaggt catcagctga cgaacaatac
    77941 cttcgtactc ctgatcgact tttttatctt cacgataaat acgtaccgct tcgtcaatgt
    78001 ccatccgcgc gaacgcgtcc agcacgtcgt gcagcatctg gatggtatga cggcccagcg
    78061 actccagact taccagcaac ggctgatgct gctgggagaa tttctccagc gcagtacggc
    78121 agattttgtc cgccacgtcg ccaatacgct ccagctcggc aatggtttta ctgatcacca
    78181 taaccagacg caggtcgctc gccgtcggct ggcgtttggc aataatgcgc acgcaggctt
    78241 catcgatcgc cacttccatc atgttgacgt tcttgtcgcc ttcgatgacg cgcttcgcca
    78301 gatcgctgtc ctggttgtgc attgcagtga tcgcatcaga aagctgctgc tccaccatgc
    78361 cgcccatggt catcacctgc gtgcggatgc tttccagttc ggcgttgaac tggccggaaa
    78421 tatgtttatt aagattgaga ctgtccataa cgcactcctg aatcaaccgt aacgaccggt
    78481 gatgtagtct tctgtttgtt tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa
    78541 ctcaatcaat tcgcccaggt acataaacgc cgtgtggtcg gaacaacgcg cagcctgctg
    78601 catgttgtgg gtgacgatca ccacggtgta atcctgcttc agttcggtaa tcagctcttc
    78661 aatacgcccg gtggagatgg ggtcgagcgc cgaacatggt tcgtcgagca gcagcacttc
    78721 cgggcgaatg gcgataccac gcgcaataca cagacgctgt tgctgaccac cagagagaga
    78781 gtaaccgctc tgatgcaatt tatctttggt ttcgttccac aatgcggctt tggtcaatgc
    78841 ccactgcacg cgctcgtcca tgtcggcacg ggagagcttc tcaaacagac gcacaccaaa
    78901 ggcgatgttg tcgtagatgg acatcgggaa cggcgtcggt ttctggaaca ccatgcccac
    78961 tttcgcacgc agcagggcga tatcctgaga gttggtcagg atgttgtcgc catcgagcag
    79021 aatttcacct tccgcacgct gttccgggta cagttcaaac attttattga aggtacgcag
    79081 cagcgtcgat ttaccgcagc cggacggccc gataaacgcc gttacctggt ttttagcgat
    79141 atccaggttg atgtttttca gggcatggaa tttgccgtag tagaagttca aattacgaac
    79201 ctgaatttta ctcggggcag tttcaaccat actcattgca atcttttcct catcgtgcct
    79261 gatgcacttc gctaatcagg cttaccgtag gccggataag gcgttcacgc cgcatccggc
    79321 aaagtgttaa ccgtgtttat tcttcgcaaa aacaacgcgc gccagaatgt tcagcagcag
    79381 tacgcacagg gtaatgatca acaccccggc ccaggccagt tgctgccatt ccgcaaacgg
    79441 gctcatcgca aacttaaaga tcgtcaccgg caggttggcg atcggctgca tcatgtccgt
    79501 gctccagaac tggttggaga gcgcggtaaa cagcagcggc gcggtttcac cggcaatacg
    79561 ggcaatcgcc agcaggatac cggtcataat cccggatacc gacgctttca gcgtaatcgc
    79621 ggagatcatc ttccacttcg gcgtacccag cgcataagcc gcttcacgca ggctgtccgg
    79681 caccagtttc agcatgttct cggtggtgcg gataacaatc ggcacctgca acagcgccag
    79741 cgcaatcacg cccgcccagc cggagaagtg ctccatctgc gccaccacga tggtgtaaac
    79801 aaacagacca actacaatcg acggtgcgga gagcagaatg tcgttaatga agcgaatcac
    79861 ttctgccagc caggatttac gaccatattc agccagataa atccccgcca taatgcccag
    79921 cggcgtaccg aataccgtgg cccacaaaat taacagcccg ctacccgcca gggcgttcgc
    79981 cagaccacca ccttccgtat tgggcggtgg cgtcatttca gtgaacagcg ccagcgacat
    80041 accatcgata ccgcgggtga tggtggacat taaaatccag atcagccaga acaggccgaa
    80101 ggccatcgtc gccatcgaga gcgtcagcgc aatacggttc ttaaggcggc gacgcgcctg
    80161 cattttgcgg cgggattccg ccagcgccgc agtggtttgc atttcaacca tagccattag
    80221 cgtgccccct cattcttagc caggcgcata atcataaact tcgatgcggc gaggacgatg
    80281 aaggtaatca caaacaggat caggcccagt tccatcagtg cggcaacgtg cagaccggat
    80341 tccgcttcgg caaattcgtt cgccagcgca gaggtgatac tgttgcccgg catgtacagc
    80401 gaggcgctgt cgagctgata ggtgttaccg atgataaagg tcaccgccat ggtttcaccg
    80461 agcgcacgcc ccagccccag catgatgccg ccgataacac catttttggt gaacggaaga
    80521 acgatacgcc agataacctc ccaggtggtg cagccaatac cgtaggccga ctctttcatc
    80581 atcaccgggg tttgttcgaa cacatcgcgc attaccgccg caatgtacgg aataatcatg
    80641 atggcgagga tcacgcctgc cgcgaggata ccgataccaa atgccgggcc agagaacagc
    80701 gcgccgacaa tcgggatatt cgacatgata ttgccgaccg gctcctggaa gtaaacggcg
    80761 aacagcggcg caaagataaa caggccccac atgccgtaaa cgatacttgg aatggctgcc
    80821 agcagctcaa tggcgatacc cagcgggcgt ttcagccagc caggcgcaag ctcagtcagg
    80881 aacagggcga taccgaaact caccgggacg gcgatcagca gcgcgataaa cgaagtcacc
    80941 aacgtaccgt agatcggcac cagcgccccg tagatatcgt ttggcgcatc ccactctttg
    81001 gtccagagga aagccagacc aaatttctga atgctcggcc aggaggagat gatcagagag
    81061 acaataatgc cacccaacat caatagcaca atcagcgccg ccagttttac cagcacgctg
    81121 aaaattatgt cgcccttttt acccggtggg ttaaaagcag gcttggttgc agccataagt
    81181 tactcttcag ttaaacgcgt ttacaaagtc attttgtaat gccggatgcg gcgtaaaaca
    81241 ccgtacccgg cctgagtttt attagtacag cggcttgccg ctactgtctt taatattggt
    81301 cttccacgca gcgcgaacct gttccactac gctatccggc aggctggcgt aatccaggtc
    81361 gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cttctgtgcc
    81421 ctgctctggt ttcttctgat ctttgtggac cagaatgaac gtggttgagg taataggcca
    81481 tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcgcc
    81541 tttttgctgc attagcgaag ttttcttcgg tcggactaac cggtttacca tcagcggaga
    81601 tcaatttggt atacgccagg ttgttctgct tagcgtaagc atattcaacg taaccaattg
    81661 cacccggcag acgctgaacg aacgcagcga taccgtcgtt acctttaccg cccagaccga
    81721 tcggccattt tacggtagag ccagtaccaa cgttgttttt ccactcttcg ttcactttcg
    81781 ccaggtagct ggtgaagacg aaggaagtcc cggagccatc tgcgcggcgt actacagcaa
    81841 tgttctgtga aggcagtttc agacccggat tcagtttggc gatggcttca tcatcccact
    81901 tcttgatttt gcccaggtag atgtcgccga gggttttacc atccagcacc agttcgccag
    81961 acttcagccc tggaatgtta accgccagca ccacgccgcc aatcacggtc gggaactgga
    82021 acaggccttc ctgagccagt ttttcgtcag acagcggcgc gtcagaggca ccgaaatcaa
    82081 cggtatttgc gataatctgt tttacgccac cggaagaacc gataccctgg tagttaactt
    82141 tattaccggt ttctttctgg taagtgtcag cccatttggc atacaccggc gcagggaagg
    82201 ttgcacctgc gcctgtcagg cttgcttctg caaacacaga gaaagcactc atcgataagg
    82261 tcgcggcgac aacagttgcg acggtggtac gcataacttt cataatgtct cctgggagga
    82321 ttcataaagc attgtttgtt ggctacgaga agcaaaatag gacaaacagg tgacagttat
    82381 atgtaaggtt tatgacagtt ttatgacaga gagagaaagt ctttagtctg atttaaataa
    82441 gcgttgatat tcagttaatt acaaatatta ataacgaaga gatgacagaa aaattttcat
    82501 tctgtgacag agaaaaagta gccgaagatg acggtttgtc acatggagtt ggcaggattt
    82561 ttgattaaaa acataacagg aagaaaaatg ccccgcttac gcagggcatc catttattac
    82621 tcaaccgtaa ccgatttcgc caggttacgc ggctggtcaa cgtcggtgcc tttgatcagc
    82681 gcaacgtggt aagccagcag ctgcagcgga acggtgtaga agatcggtgc aatcacctct
    82741 tccacatgcg gcatctcgat gatgtgcatg ttatcgctac ttacaaaacc cgcatcctga
    82801 tcggcgaaga catacaactg accgccacgc gcgcgaactt cttcaatgtt ggatttcagt
    82861 ttttccagca attcgttgtt cggtgcaacg acgataaccg gcatatcggc atcaatcagc
    82921 gccagcggac cgtgtttcag ttcgcctgca gcgtaggctt cagcgtgaat gtaagagatc
    82981 tctttcagct tcaatgcgcc ttccagcgcg attgggtact gatcgccacg gcccaggaac
    83041 agcgcgtgat gtttgtcaga gaaatcttct gccagtgctt caatgcgttt atcctgagac
    83101 agcatctgct caatacggct cggtagcgcc tgcagaccat gcacgatgtc atgttcaatg
    83161 gaggcatcca gacctttcag acgagacagc ttcgccacca gcatcaacag cacggttaac
    83221 tgggtggtga atgctttagt ggacgccacg ccgatttctg tacccgcgtt ggtcattagc
    83281 gccagatcgg attcgcgtac cagcgaagaa cccggaacgt tacagattgc cagtgaacca
    83341 aggtaaccca gctctttcga cagacgcagg ccagccaggg tatccgcggt ttcgccagac
    83401 tgtgacaagg tgatcatcag gctgttacga cgcacggcag atttgcgata gcggaattca
    83461 gaggcgattt cgacgtcgca cggaatacct gccagcgatt caaaccagta gcgggaaacc
    83521 ataccggagt tataagaagt accacaggcg aggatctgaa tatgctcaac cttcgacagc
    83581 agttcgtcgg cgttcggtcc cagctcgctt aaatcaacct gaccgtggct gatgcgcccg
    83641 gtaagggtgt ttttgatcgc gttcggctgt tcgtagatct ctttctgcat gtagtggcgg
    83701 taaatacctt tatcgcccgc gtcatattgc agattggatt cgatatcctg acgttttact
    83761 tccgcgccag ttttatcgaa gatgtttacc gaacggcgag tgatttccgc aatatcgccc
    83821 tcttcaagga agataaagcg acgagtcact ggcaacagcg ccagctggtc agaagcgata
    83881 aagttttcgc ccatacccag gccaatcacc agcggactac cagaacgtgc cgccagcagg
    83941 gtatccggat gacgggagtc catgatcact gtaccgtacg caccacgcag ttgcgggata
    84001 gcacgcagaa cagcctcacg cagagtaccg ccttgtttca actcccagtt caccagatgg
    84061 gcaatcactt cggtgtcggt ttcagaaacg aaggtatagc cacgcgcttt taactcttca
    84121 cgcagcggtt catggttttc gatgatgccg ttatgcacca ccacaatgtg ttcagaaaca
    84181 tgcggatgcg cgttagcttc tgaaggttca ccgtgggtcg cccagcgagt atgagcaata
    84241 ccggtgccgc catgcagagg atgttcttcc gctgcctgag ccagcatctg gactttaccg
    84301 aggcgacgca ggcgggtcat atgaccttct gcatcaacaa cggccagacc ggcagagtca
    84361 tatccgcggt attcaagacg acgtaaacct tcaagaagga tttctgctac atcacgttgc
    84421 gcgatcgcgc caacaattcc acacatagtt tttgattccg atttatatcg ttgtcggtca
    84481 acctgtatgc ccgttttttc gggcaccccg agccttgtag agagtggggt tatttttata
    84541 gttactgctt gtgggcggga gattatgtta tctcctcatc ccatatgacc gtattacccg
    84601 gccagaatta ctttttcttt accggacgac gccagccttc tttttgagtc tgcggcacac
    84661 ggctgatcgc cagggcgttt tcgccgacat tacgcgtcac agttgtaccc gcagcaatgg
    84721 tcgcgccttt gcctactgtt accggggcca ccagctgagt gtcggaaccg acaaacacat
    84781 cgtcaccgat aatggtctta aatttattcg caccatcgta gttgcaggta atggttcccg
    84841 cgccgatgtt aacgttatcg ccaatttccg catcgcccag gtaagtcaga tgcccggctt
    84901 tcgagccttt acccagacgc gcttttttca tctcaacgaa gttaccgacg tgtgcacctt
    84961 ccagcaactc agcaccagga cgcagacggg caaacgggcc aatggtacag gccgctgcca
    85021 gattcgcatc ttccacaacg gtatacgggc taatttcgca atcatcgcca atcacgctgt
    85081 ttttaatcac gcaaccggtg ccgattttca cgcgatgacc gagagtcacg ttgccctcga
    85141 tgataacgtt agtatcaatt tcaacatcgc gcccgtgagt aagcgtaccg cgcagatcaa
    85201 aacgcgccgg atcgcgcagc ataacgcctg ctaacagcag tttttcagcc tgttcggact
    85261 ggtaaacgcg ctccagtcgg gagagttgca ggcggttatt cacgccttct acttcgctta
    85321 aacgttgcgg atgaacggcg acgatttcac gcccttcctg atacgccagc gcaataatgt
    85381 cggtgatgta gtattcaccc tgagcgttat tgttggtcag cttcgccagc cagcgtttca
    85441 tatctgcgcc gttggcaatc agaatgccgg tgttgatctc ctgaatctga cgctgctcgt
    85501 cggtggcgtc tttgtgctca acaatgccgg taactttgcc gttttcacgg gtgatacgtc
    85561 cataaccggt cggatcatcc agttttaccg tcagcagacc aatgccaccc tgcggtttag
    85621 catcacgcag gcgctggagt gtttcgacag agatcagcgg cacgtcgccg tagagcatta
    85681 aaatgtcttc atcatcggca aagaaaagtg cggcctgctg catcgcatga cctgtaccca
    85741 gctgttctgc ctgaagcacc cagttcaggt tgtcatcttt cagcgcctgt ttaagcagat
    85801 cgccgccgtg accgtacacc aggtgaacgt gcgccgcgcc taattcattc gcagcatcaa
    85861 tgacatgctg aaccatcgct ttcccggcaa gggtatgcag cactttcgga agatcggaat
    85921 acatgcgcgt gcctttgcct gcggcaagga tcactacgct catagcatta ttcaacatac
    85981 gcgtcctgac tgtaatttga gaacgaattt aaaccgcttc accttgaaaa aactacattt
    86041 ttttcatcgt gaaatggaca gaggataaat tgttcaataa cggattatcc ccgctgacga
    86101 aagcgccatt ttcgaccatc gtcacgcctt ttgtctgcag aaaaataagg caataccttc
    86161 atttaagcgt caggtggatg tttttgctct tatttcgatc aatgaataaa cgggaaaaca
    86221 gggtgtttta tatcagcagg atctatgtga acgctattca ggacggggca acgcgtaaaa
    86281 aaaagccagc ctgtttccag actggctttt gtgcttttca agccggtgtt acatcgcttt
    86341 tttggtcaac tcgataacgc gcagctgcgc gatcgctttg gccagttccg cagacgcctg
    86401 agcgtaatct acgtcgccgt gagagctgct aatgtgctct tcagccttac gtttcgcttc
    86461 catggctcgc gcttcgtcga gatcctggcc gcgaattgcg gtgtcggcca gaacggtcac
    86521 gttgccaggc tgcacttcaa gaatgccgcc agacagatag ataaactctt cgtgaccgtg
    86581 ctgtttcacg atgcgaatca taccaggctt aatggcggtg agcagcggtg cgtggccagg
    86641 gtagatcccc agttcacctt cgctacccgt tacctggatt ttctcgacca gaccagagaa
    86701 catttgttgc tctgcactga cgacgtccag gtggtaagtc attgccatat caccctccga
    86761 ttaaggcgtt aaagtttttt ggctttttcc acagcttctt cgatggaacc gaccatgtag
    86821 aacgcctgct ccggcaggtg atcgtattcg ccttccatga tgcctttaaa gccacggatg
    86881 gtgtctttca gggagacgta tttacccgga gaaccggtga atacttctgc cacgaagaac
    86941 ggctgggaca ggaagcgctg gatcttacga gcacgcgcta ccaccagttt gtcttcttca
    87001 gacagttcat ccatacccag gatggcgatg atgtctttca gttcctgata acgttgcagg
    87061 atggactgaa cgccacgcgc ggtgtcgtag tgttcctgac caaccaccag cgggtccagc
    87121 tgacggctgg tggagtccag cgggtcaacg gccgggtaga tacccagaga cgcgatctga
    87181 cggctcagta ccacggttgc gtcaaggtgc gcaaaggtgg ttgccggaga cgggtcagtc
    87241 aagtcatccg caggtacgta taccgcctgt acggaggtga tagaaccagt tttggtggag
    87301 gtgatacgtt cctgcagaac gcccatctct tccgccaggg tcggctgata acctaccgct
    87361 gaaggcatac ggcccagcag tgcggatact tccgtaccgg ccagggtgta acgatagatg
    87421 ttgtcaacga acagcagaac gtcacgacct tcgtcacgga atttctcagc catggtcaga
    87481 ccggtcagag caacgcgcag acggtttccc ggcggctcgt tcatctggcc atacaccagg
    87541 gatactttgt cgataacgtt ggagtcggtc atttcgtggt agaagtcgtt accctcacga
    87601 gtacgttcac ctacgcccgc aaacacagag taaccggagt gctcgatcgc gatgttacga
    87661 ataagctcca tcatgtttac ggttttacct acacccgcac caccgaacag accaacttta
    87721 ccgcccttag cgaacggaca catcaggtcg ataactttga taccggtttc cagcagttcc
    87781 tgagagtttg acagctcttc gtaggaaggt gctgcgcggt gaatcgccca acgctcttct
    87841 tcaccgatct cgcctttcat gtcgaccggt tcacccagta cgttcatgat acggcccaga
    87901 gtcgctttac ctaccgggac ttcgatcggg tgttcgaggt cttttacatc cagaccgcga
    87961 cgcagaccgt cggaggaacc cattgcgatg gtacgtacga taccgccgcc gagctgctgc
    88021 tgaacttcca gcaccagacg ctcattacca ttttgcacct caagagcatc gtacacgcgc
    88081 ggtacggcat cctgagggaa ttcgacgtca actacggcgc cgattacctg gacaatcttt
    88141 ccagtagcca tcttaaatcc tctacgaaat aacctgttta aaccgcggcg gcccccgaga
    88201 cgatctcggt gagttcctga gtaatgctgg cctgacgagc tttgttgtat accaactgca
    88261 gctctttaat caggctgccg ccattgtcgg tcgcggcttt catcgccacc atacgggcgg
    88321 cctgctcgct ggccaggttt tcaaccacgc cctgataaac ctgagattcg acataacgac
    88381 gcagcagggt atccagcaac gccttcggat cgggttcgta caggtaatcc caggatttat
    88441 gtttcagatc atcatcatct gatgccggta acggcagcag ctggctgatg gtcggaacct
    88501 gagacatggt gttaataaat ttgttgctga caatgtaaag cttgtccaga cggccttcgt
    88561 cgtaggcctg caacatcact tttaccggac cgatcagttc ggacagggaa gggttatccc
    88621 ccatgccggt gacctgggca acaacattgc cgcccacgga gttgaagaac gacacgcctt
    88681 tcgagccgat cattgcgagg tcgcattgaa cgcctttgtc ggtccaggtc ttcatttccg
    88741 ccagcagttt tttgaacagg ttaatgttca aaccaccgca caaaccacgg tcggtcgaca
    88801 ccaccaggta gcccacgcgt ttaacgtcgc ggtcttccag gtaagggtgc ttatattcca
    88861 gattaccgtg tgcaaggtga ccaatcactt tgcgcatggt ttctgcataa ggacggctgg
    88921 ccgccatgcg atcctgcgat ttacgcattt tggaagcggc gaccatctcc atcgctttag
    88981 tgatcttttg cgtgttctgg acgcttgcga tcttactacg tatctctttt gcgccggcca
    89041 tgagcttctc ctcaatgcct tgcggcctgc cctaaggcaa gccgccagac gttaccagga
    89101 ttgggttgct ttgaaggaat cgaggatgcc tttcagcttg ccttcgattt cgtcgttgta
    89161 gccaccggtc tggttgatct cttgcatcaa cggagcgtga tcacggtcga cgtaagccag
    89221 cagagcggct tcgaagctgc cgattttcga cagttcaaca tccgccaggt aaccacgttc
    89281 tgctgcgaac agaaccagag actgttgcgc tacggacatc ggcgcatact gtttctgttt
    89341 cagcagttcg gtcactttct gaccgtggtc aagctgctta cgtgttgcat cgtcaaggtc
    89401 ggatgcaaac tgagagaacg ctgccagttc acgatactgt gccagagcgg tacggatacc
    89461 accggacagt tttttcatga tcttggtctg tgctgcacca ccaacacggg atacggaaat
    89521 acccgggtta accgcaggac gaataccggc gttgaacagg ttggtttcca ggaagatctg
    89581 accatcggta atggagatta cgttggttgg aacgaacgca gaaacgtcac ccgcctgagt
    89641 ttcgataatc ggcagcgcgg tcagagaacc ggttttccct ttcacttcac ctttggtgaa
    89701 ggcttcaacg tattcggcgt taacacgcgc agcacgctcc agcagacgag agtggaggta
    89761 gaatacgtcg cccgggaatg cttcacgtcc tggcggacga cggagcagca gggagatctg
    89821 acggtaagca acagcctgtt tagacaggtc atcgtaaatg atcagcgcat cttcaccgcg
    89881 gtcacggaag tattcgccca ttgcgcaacc agcgtacggt gccaggtatt gcagtgcagc
    89941 ggattcagac gcggttgcta ccacaacgat ggtgttagcc agtgcgccgt gctcttccag
    90001 tttacgtacc acgttagaaa tggtggacgc tttctggccg atagcgacat agatacattt
    90061 gataccggaa tcgcgctggt tgatgatggc atcgatagcc agtgcggttt taccggtctg
    90121 acggtcaccg atgatcaatt cacgctgacc acgaccgatt gggatcatgg agtcaacggc
    90181 tttataaccg gtctgtaccg gctgatctac ggactgacgt tcgataacgc ccggagcgat
    90241 tgcttctaca gcagagaagc cgtcgtgatc cagcggacct ttaccatcga ttggtgcacc
    90301 cagagtgtta accacacggc ccagcaggcc acggccaacc ggaacttcca ggatacggcc
    90361 agtacactta actttcatgc cttcggcaag gtcagcgtac ggacccataa caaccgcacc
    90421 tacagagtcg cgctcgaggt tcagtgcgat agcgtaacgg ttacccggca gggagatcat
    90481 ttcaccctgc atacaatcgg ccaggccgtg gatgcggata acaccgtcac ttacagaaac
    90541 aatagtacct tcgttgtgag cttcactcac aacattgaac tgagcaatgc gctgcttgat
    90601 cagttcgctg atttcggtgg aattcagttg catgctccag tccccttaag actgcaagac
    90661 gtctgcaagg cgctcaagac gaccgcgtac gctgccatca atgaccatat cacccgctcg
    90721 gatgataacg cctgccatta cagacttatc gattttgcaa ttcagcttaa ctttgcgtga
    90781 cagacgtttt tccatcgcag cagaaatttt cgcgagctgt tgttcactca gtgcggcagc
    90841 ggaaatgacg tctacctcag cggtagcctc actcacggca cgcaggtgaa taaactgctc
    90901 cagaacatcc gggagcgcgt taagacgacc attttcggcc attacccgaa tcaggttctg
    90961 accgttttcg tccagttgct caccacaaac tgcgataaac gactcggcga gcgtttctgg
    91021 cgcaagcgcg ccagagagaa gctctgccat ttgttcgttt ttggttacct cggcggcaaa
    91081 cgccagcatg tcctgccagc gttctacact ttggtgttcg acggcaaagt caaaagctgc
    91141 tttggcgtag gggcgagcta ccgtaataaa ttcagacatc agcccctccc tccttacagt
    91201 tcagcgacaa gtttatccac gatgtcgctg ttagcagctt catccacgga acgttcgatg
    91261 atcttctcgg cgccagcaac agccaggata gcaacttgct tacgcagctc ttcacgggca
    91321 cgtttacgct cggcttcaat ttccgcctgc gcctgggcca cgattttagt acgttcctgt
    91381 tctgcctcag ctttcgcttc gtccagaatc tgcgagcggc gtttgttagc ctgctcgatg
    91441 attacctggg cttccgcttt cgcttttttc agctggtcgg tcgcgctggc ctttgcaagg
    91501 tcaaggtcct tatgtgctcg ttctgcggaa gcaaggccgt cagcaatttc tttttgacgt
    91561 ttttcgatgg ctgccattaa tggcggccat acgtacttca tgcagaacag aacgaacagg
    91621 acaaacgcga tggcctggcc gaggattgtt gcgttaagat tcacagcaca atgcctctat
    91681 ttagttaacg ttctgatatt gctctttaaa taaaagcaac gcttactacg cgacagcgaa
    91741 catcacgtac agacccagac ctacagcgat catcgggata gcatccacca gacccataac
    91801 gataaagaac tgagtacgca gcagaggaat cagatcaggt tgacgcgctg cgccttccag
    91861 gaatttaccc ccgaggatgc cgataccgat cgcagcaccg attgccgcca gacccatcat
    91921 cacagcggca gccatgtaca gcagatccat attcaggttt tccatgacag tctccagttt
    91981 gtttcagtta aaacgtagta gtgttggtaa attaatgttc ttcagacgcc atcgacagat
    92041 agacgatcgt cagaaccatg aagatgaagg cttgcagcgt aatgatcagg atgtggaaaa
    92101 tggcccacgg cacattcaga atccactgtg accaccacgg caacagacca gcaatcagaa
    92161 tgaaaatcag ctcaccggca tacatgttac cgaacagtcg caaaccgagt gaaactggtt
    92221 tggacagcag gcttacccct tcaaggatta agttgacagg aatgaacgcc cagtgattga
    92281 acggctgcag cgtcaactct ttcgtgaagc cgccgatgcc tttcattttg atgctgtaga
    92341 acagaatcag gataaataca cccagtgcca tagacagcgt tacgttcacg tccgcagacg
    92401 gaaccacacg cagtgcaggc agacccagta catgttcagc aatgtacggc agcaggtcga
    92461 taggcagtaa atccatcagg ttcatcagga atacccagac gaagatcgtc agggccagcg
    92521 gagcaatcag cttgcttttg ccatggtaca tgtctttcac gctaccatta acaaagccga
    92581 tcaccagctc aatcgcggtc tgaaacttac ctggcacacc gctggtcgcc tttttggcta
    92641 cgctacggaa taaaaccagg aacaacagac ccagcaccac cgagaagaac atggagtcaa
    92701 tattgattgt ccagaaggtg gctggggggt tatgtggatc caccagcgag aatgtacgca
    92761 ggtccagctg aaggttgttc aggtggtgtc ctatgtaatc ctgcggcgtc atattttctg
    92821 aagccatgat gccttttacc ctttgttgtt aattacagcc ggtgccagta tctgaaccac
    92881 cagcaccaaa acccacgtaa cgatcagcgg caagaatacc gcctttaaaa ccgccaacgc
    92941 caccaccagt aacaccaaca tcgccagaac tttgaaagct tcgccaaatg cgaatgtcca
    93001 ggccacccgg cctttcgctg gtgtatgcgc ctggtgacgc caggcaaata tcataaacaa
    93061 aacgttaggc agaaagactg ccaggccccc gcttattgca gagacgcccc agaaggggtc
    93121 tttgaggctg aacagcaatc cacttgctat caccaccagt aactgaacga gcagaagctt
    93181 ccgagcaacg tttcgactca cgagcgacac agacatcacg tttttcactc ctgctccctt
    93241 cgaggtatgc cgcgtgtcgt ataaaacttt ctttaaggct tagagtcaag catcaaaaag
    93301 cggtcaaatt atacggtgcg cccccgtgat ttcaaacaat aagtagccaa aaggtgaata
    93361 aatgtttaaa tatttttcca gtgcatacaa ttgcgacttt tctgcaaacc ctgttcgatc
    93421 atgaaaaact gtaaataacg cgtaaacact ggtgataaag cgtgcttcag atcacatatt
    93481 gagcatgttc gcgcacagca tatttattta cttggcaaat gatgcctttg caagtttatg
    93541 atatttcagt ctaaaaacag atactgtttt aataaatgac atttacacaa caaaaaccac
    93601 ccattgacat ttttaataat gttttaacag caaatgatga ttcttagcgc cgatttttag
    93661 cagactgata ttttcactta tgacttattt tctgcttacc aaaaaaagcc acgttacttt
    93721 gttgatgcaa aagagtgaac gtggcgttaa atgtaaccag ttatatcagt agaaaacctg
    93781 gttgttgtta acagtctaac cggtcaattt tttatgattt ttttgataaa aattaaattt
    93841 tatttgcttt aatcaccacc agatgacgtt cgccatccag ggccggaacg tgaagtttaa
    93901 ccactgattc gacctgatat tcttcgggca acaaagcgat ttcatcttcc ggcatttggc
    93961 ctttcagcgc gtagaaacga ccttgctcac caggaagatg gtggcaccag ctcaccatat
    94021 cgttcagaga ggcaaaagcg cggctaatta cgccatcaaa tggcggctct gaaggaaact
    94081 cttctaccct gctctgtact ggctcaatat tctccagttt aagctcatgt tgcacctgac
    94141 gaaggaaacg cacgcgttta ccaaggctat ccaacaacgt gaagtgggct tcaggacgca
    94201 cgatagagag tggaatgcca ggcagtcctg gtccggtgcc gacatcgata aaccgttcac
    94261 cttgcagata cggtgccacc acaatgctat cgagaatatg gcgtaccagc atctcattag
    94321 gatcgcggac cgaagtcagg ttgtacgctt tattccattt atgcagcata ttcacgtagg
    94381 caattagctg gtttttctgg tgatcggtaa gcgaaattcc tgcgtctttc agcagagagg
    94441 tgagtttgtt gagcacggtg attacctgtt cttgatgcgt tgcccggtaa gcgggtgctt
    94501 accaggcatt tttaatgcgt tatgcgctac gacgcagcat accctgtttt ttcagccata
    94561 ccagcagaat ggagatggcc gcaggcgtga cgccagaaat acgcgaagcc tggccgatag
    94621 aggccggttt gtgatcgtta agtttggcga tcacttcgtt agaaagaccg gatacctggc
    94681 ggtaatccag cgtcgctggt agcagggtgt tctcgttacg cagctgcttt tcgatctcat
    94741 cttgctggcg cgcgatataa ccttcgtatt taacctgaat ctcaacctgt tccgccgcct
    94801 gttcgtctgt caacgcaggg gcaaacggcg tcagcgtggt taatttttca taagtcattt
    94861 ccggacgacg cagcagatct tcaccactgg cttcacggga aagcggcgca gtcaggtgag
    94921 cattcacttc ggctgcagct tccgccgacg gggttaccca ggtcgatttc agacgctgac
    94981 gctcacgctc gatattctca agtttctcgt taaagcgcgc ccaacgttcg tcatccacca
    95041 ggcccagttc acgaccgatt tcagtcaaac gcagatccgc attatcttcg cgcagcatca
    95101 gacgatattc tgcgcgcgaa gtaaacatac gatacggttc tttggttcct aaagtgcaca
    95161 ggtcatcaac cagtacgccg agatacgcct gagaacgcgc cggagcccaa ccttctttgt
    95221 cagcagacag acgggcagcg ttaagaccag ccagcaaacc ttgcgcagcg gcttcttcgt
    95281 aaccggtagt gccgttaatc tgaccagcaa agaacagccc ctggataaac ttgctctcca
    95341 gcgtcggttt caggtcgcgt ggatcgaaga agtcatactc aatggcataa cccggacgta
    95401 cgatcttcgc gttttccatc ccctgcatgg agcggacgat ttgcatctgc acatcgaacg
    95461 gcaggctggt ggagataccg ttcggataaa tttcgtttga ggtcagccct tccggttcaa
    95521 ggaagatctg atgctgattt ctgtcggcga agcgcatgac tttgtcttcg atcgacgggc
    95581 agtagcgtgg gccgacacct tcgatcaccc ctgcatacat tgggctacga tcgaggttac
    95641 tgcggatcac atcatgggtt ttctcgttgg tatgggtgat ataacacggc acctgctgtg
    95701 gatgctggga cgcattgccc ataaacgaga ataccggcat tgggttatcg ccatgctgtt
    95761 gcgccagcac gctgaagtcg atagtacgcg catcaatacg cggcggtgtc ccggttttca
    95821 gacgaccaac gcgcagcggc agttcacgca aacggcgaga gagcggaatg gacggcggat
    95881 caccagcacg gccaccgctg tagttatcca gaccgatatg aattttaccg tcgaggaacg
    95941 tcccaacggt gagcacgacg gctttggcac ggaacttcag tcccatttgg gtaacagcac
    96001 cgaccacgcg atcgttttcg acaataagat cttcaaccgc ctgctggaag atcatcaggt
    96061 tcggttggtt ctccagcgcc gtacgtaccg cctgccggta gagcacacga tccgcctgag
    96121 cacgggtagc gcgaaccgct ggccccttgc ttgcgtttag tatcctaaac tggatacccg
    96181 cctgatcgat cgctttcgcc atcagaccgc cgagtgcatc cacttctttt accagatgtc
    96241 ccttcccaat accgccgatc gccgggttgc agctcatctg ccccagagtg tcgatattgt
    96301 gtgtcaaaag cagagtctgt tgacccatac gcgccgcggc catcgcggcc tcggtgcctg
    96361 catgaccccc gccaatgatg atgacgtcaa aaggatccgg ataaaacatg gtgattgcct
    96421 cgcataacgc ggtatgaaaa tggattgaag cccgggccgt ggattctact caactttgtc
    96481 ggcttgagaa agacctggga tccagggtat taaaaagaag atctatttat ttagagatct
    96541 gttctattgt gatctcttat taggatcgca ctgccctgtg gataacaagg atccggcttt
    96601 taagatcaac aacctggaaa ggatcattaa ctgtgaatga tcggtgatcc tggaccgtat
    96661 aagctgggat cagaatgagg ggttatacac aactcaaaaa ctgaacaacg gttgttcttt
    96721 ggataactac cggttgatcc aagcttcctg acagagttat ccacagtaga tcgcacgatc
    96781 tgtatactta tttgagtaaa ttaatccacg atcccagcca ttcttctgcc ggatcttccg
    96841 gaatgtcgtg atcaagaatg ttgatcttca gtgtttcgcc tgtctgtttt gcaccggaat
    96901 ttttgagttc tgcctcgagt ttatcgatag ccccacaaaa ggcgtcatat tcacgactgc
    96961 caataccgat agcgccaaag cggaccgcag aaagatcggg cttctgttcc tgcaatgctt
    97021 catagaaagg agaaaggtta tccggaatat ctccggcacc gtgggttgag ctgataacca
    97081 gccagatccc cgaggcagat aaatcttcca acagcggacc gtgcagcgtt tcggtggtaa
    97141 aacccgcctc ttccagcttt tcagccaggt gttctgccac atattcggca ccgccgaggg
    97201 tgctgccgct gataagagtg atatctgcca taaaccgcca cctttattaa gagtggcgta
    97261 ttgtacgctg tgaacgcgtt gggatctacc tgtggaaaag tatgggatta aaaaagccga
    97321 tcagggctta atggtacgca tgatcgggtt ctgcaggacg atcagtgtct cggtggactg
    97381 aatttcatca attgtttgga tcttgttgat aagtacatgc tggagagcat cgatcgaacg
    97441 gcacatcact tttataaaga tgctgtagtg gccggttgtg taataggctt cagtgacttc
    97501 atcgaggctt tccagctttg ccagcgcgga aggatagtct ttggcgctct ttaatataat
    97561 gccgataaag cagcctacgt cataaccaag ctgcttcggg ctgacatcaa tacgcgcccc
    97621 ggtaatgatt cccgcctgct tcattttctc tactcgaacg tgaatcgtcc ccggactgac
    97681 gccaaattgt ttcgccagtt cggcgtaagc ggtgcgcgca ttgcccatta atgcttccag
    97741 gatgccacgg tccagattgt cgattagata attttccata ggattttctt atgcggattg
    97801 atgattcatt ctattttagc cttctttttt aatgaatcaa aagtgagtta ggctttttat
    97861 tgaatgatta ttgcatgtgt gccggttttt gttgcttaat cataagcaac aggacgcagg
    97921 agtataaaaa atgaaaaccg cttacattgc caaacaacgc caaattagct tcgtgaaatc
    97981 tcacttttct cgtcaactgg aagaacgtct ggggctgatc gaagtccagg cgccgattct
    98041 tagccgtgtg ggggatggca cgcaggataa cttgtcgggc tgtgaaaaag cggtgcaggt
    98101 aaaagtaaaa gctctgcctg atgcccagtt cgaagtggtt cattcactgg cgaagtggaa
    98161 acgtcagacc ttagggcaac acgacttcag cgcgggcgaa gggctgtaca cgcacatgaa
    98221 agcccttcgc cccgatgaag accgtctttc tccgttgcac tcggtctatg tagaccagtg
    98281 ggactgggaa cgcgtaatgg gcgacggcga gcgtcaattc tcgactctga aaagcacggt
    98341 agaggcgatc tgggcgggaa ttaaagcaac cgaagctgcg gttagcgaag agtttggcct
    98401 ggcaccgttc ctgccggatc agatccactt cgtacacagc caggagttac tgtctcgtta
    98461 tccggatctc gatgccaaag ggcgtgagcg ggcgattgcg aaagatcttg gcgcggtatt
    98521 cctcgtcggg attggcggca agttgagtga tggtcatcgt cacgacgtgc gcgcaccgga
    98581 ttatgatgac tggagcaccc cgtcagagct gggccatgcg ggtctgaacg gcgatattct
    98641 ggtatggaac ccggtactgg aagatgcgtt tgagctttct tccatgggga tccgcgtgga
    98701 tgccgatacg ttgaagcatc agctggcgct gaccggtgat gaagatcgtc tgcagctgga
    98761 gtggcatcag gcgctgctgc gcggtgaaat gccgcagacc atcggcggcg gtatcggcca
    98821 gtctcgtttg accatgctgc tgctgcaact gccgcatatc ggccaggttc agtgtggtgt
    98881 atggccggca gctgttcgcg agagcgtccc ttctctgctg taattgttta tcgccgccag
    98941 cgtctgagca ggcggcttcg catcccggta tcaaagcgcc agatatgatc gaaaatgcgc
    99001 atgatgccgg gtttgccatg tgccgacatc gccacggcat gaaagcgatg ctgatgtacc
    99061 cgctgcaact ctttcacttt gctcgtcacg tcgtcaggca accgctgggc gataaaatca
    99121 gaaatcacca ccgcatcggc gtcaaaccac tcccgacttt gcaagcgttc cataatggcg
    99181 cgaaaacaac tggccagatc ggtgccgcca cgaaactgct gacttaaaaa gcggatcgct
    99241 tgttcgatgc cttgtgggcc tgaaagctca taacggacga tctcggtgga aaatagcata
    99301 atatagcagc gccggttttc agcgagagca atgcgcatca aggccaggca gaacgctttc
    99361 gcacactgtt cattaaagcc gcccattgag ccggaagtat ccacacagac aataaacggt
    99421 ccgcgcggct gttcgtcgta atctttatgt accaccggac gttcgatcac tttttctcgc
    99481 cacgactcac cgtgcaggcg ataggtgagc aactgttttt ccaccagccg acggtaaaac
    99541 tcatactcca gttctgttat ccctagtgtc gccagttctg gcggcaggag acgtaaaata
    99601 tcatcgcttt gttgcagacc atcaacctgc tcaggaaccg tcgccggttc gcgcaccatg
    99661 gtgcggaagg tttccatctg cgcatcgttg cgcggtattg atttggcttc ccgggaacgc
    99721 cccagctgtt ctgccaggcg tttcagttcc ggctgttcgt taagaaattc accgtatttc
    99781 acaatcaact gatagtcgcc acgtttaagc tgaccggcgc tcatatccca cagacgacca
    99841 gctgcggtat tgttatctgc gagaatcggt tcaagttgtc cgctcagcgt catgcgttcc
    99901 tgaacttcac tcaacagttg ttcgcgttct tcttctaata gctgttgatt taacgtcgtt
    99961 gcttgcacga tcagacttaa acgccaacgc tggagaaaaa gcgtgtgtaa cgctgaagtg
   100021 atcgtgctgt tagcatcaac caactgtcgg gcttgttctg cccatgggga attcagacga
   100081 tgcagtaagt ccaggatctg tggtagctgc acaataaact gcggcgtgga gaggagctgg
   100141 ctttgctgat agcacatcac ctcttcggtg agttccggcg ggactcgggc atctttcagc
   100201 cgactgcgca gcgcctcacg ccagcgggga acatcatcag tgattgccgc cttcagtcgt
   100261 gggaattttt caaagaagac tgccagctgc ggtgaggcca gcagcgcgat gatcatctct
   100321 tcgatcaatc cctcttcgct gacggccagc atcacattaa gcgtatccag cgttagcatt
   100381 gttgtgcctg gcgaatctgt gcgccgacat cctgtaggct ggcttcaatg cgacctaacc
   100441 agtcgccagg gataaacaga catttttgct gttcgctgaa taaagcgtgt tgcttgcgcc
   100501 agtcgctttc aagctcttcc agttgttgtt ttatttcacc tggcagacct tcagccgatg
   100561 aaccgggaag tgccaacgta ctgccttgta aacttacatc gcgtacaaca agatgttggg
   100621 cgctatccac ttccagattc aatttctgag caaagccgat accgttcagt ttgccgcgaa
   100681 tttcaccacc tttactcagc cactgttcca gcgcgttacg ctcaaaggag atatgaacca
   100741 cttccatatc atgcagtttt aacggttttt gcagcagcag agtcagagtg gaagcagtaa
   100801 cgttaacagg gagttgatac tgctgacgac ggctgaaaat gccgcccaga cgaattaccg
   100861 ttaaggctgt tttatcgctt tgttgctgct gtagttgcag gtgacgttgc acaatcgcgc
   100921 ccaggcgggt caacatccct tgctgttgcc aggcatgacc ggtcatcaat acatcaattt
   100981 gttgttgtat caaattcagg ctttgcgcgt cataccacag gcaatctttc agcaaaatga
   101041 gatcaaccgg ggcaacagca ctgcgaccgt taaaaaaggc gctggcctgc aataagcgga
   101101 tcgctttttt ccagcgacga tccgagacat aaggcgcatc cggtaattta tccagttgct
   101161 ggcgcagcat aaaaatcagc tcaaatacat gatcgggcag cgtaatttca ccaatctctt
   101221 tctgccagcg ttcatattct tcatctgtga cctgcaagga ggcaggaacc ggattgtcgt
   101281 tttcatcctg ttgactggtc agcatggagc ggaaattcgc tttatcctgc actttatcta
   101341 accacagacg aatcagcatg cggtcatata acgcttccag actgctgtct gcttccggca
   101401 gctcgttgga ggccgccacc agcaggcgca tcgggatttt ttctacgtgt gcaccgttgc
   101461 ggaactggcg ctcgttaatg gcggtgagca aggtattaag aattgccggg cccgctttcc
   101521 agatctcatc cagaaagacg atttctgctt ccggcaggta accgctggtt aaacgttcat
   101581 agcgcccttc atcttttagc gcctgaatag aaaggggacc aaaaacctct tccggcgtgg
   101641 agaagcgggt catcagatat tcaaacgcgc gggcattctg aaaggcgaat tttaagcgcc
   101701 gggcgatcaa acttttggca atgcctggcg ggccaaggag gaacacactt tcaccactta
   101761 atgccgctaa taaacacaag cggatggcgt ggctacgttc ataaagcccc ttttccaacg
   101821 aactgctcag gcgggaaatt ctttccgcta ataaatgagg gtgagccata atgaagtggc
   101881 gtcctttcgt caaaagttct gcgtaaattg cgagtataga cgtttcttgc tggtggctaa
   101941 aatagtctca aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag
   102001 tacgattttg ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca
   102061 cacatttcat atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc
   102121 cgcgattacc ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata
   102181 tacacttcgt gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt
   102241 tggcttttta tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac
   102301 cttcgtgatg cgggcagata acgccggtga agggggggat cctgacgttg atgtcgcttg
   102361 ccgggcgtaa tacgtcggcg cgaaccacat caatgctggt gattatgggg ctaatcggcg
   102421 gcagcttttt ctatggtgaa gtcgtcataa cacccgctat ttcggtgatg tcagccattg
   102481 aaggtctgga aatcgtcgcc ccgcagctgg atacctggat agttcccctc tcaatcatcg
   102541 ttctcacttt actatttatg attcaaaaac atggcacggc tatggtcggt aagctgtttg
   102601 cgccgatcat gctgacctgg tttttgattc tggcagtact ggggttacgt agcattattg
   102661 ctaacccgga agtgctgcat gcgctgaatc cgatgtgggc ggtgcatttc ttccttgaat
   102721 acaaaacggt ttcttttatt gcattagggg cagtggtgct gtcgattacg ggggtcgagg
   102781 cgctgtatgc tgatatgggg cactttggta agttccctat tcgcctggcg tggttcaccg
   102841 tcgtattacc ttccttgacc cttaattact tcggtcaggg agcgttgttg ttaaagaacc
   102901 cggaagcgat taagaacccg ttcttcctgt tggcaccgga ctgggcgctg atcccgctgc
   102961 tgatcatcgc cgcactggcg acggtaattg cctcgcaggc ggttatctct ggcgtcttct
   103021 cattgacgcg tcaggcggta cgtctgggat atttgtcgcc gatgcgcatt attcacacct
   103081 ccgaaatgga gtcagggcaa atctatattc cgtttgtgaa ctggatgctc tatgtcgcgg
   103141 tcgtgattgt gattgtcagc tttgagcact ccagcaacct ggcggcggcg tacgggattg
   103201 cggtgaccgg aaccatggtg ctgacgtcta ttctctcgac taccgtggcg cgtcagaact
   103261 ggcactggaa taagtatttt gttgcgctga tcctgattgc tttcctttgt gtcgatattc
   103321 cattgttcac cgctaacctc gataaactgc tctccggcgg ctggttgcca ttgagcctcg
   103381 gtacggtgat gtttatcgtg atgaccacct ggaagagcga acgtttccgt ttgctgcggc
   103441 ggatgcatga acatggtaac tctctggagg cgatgattgc ttcgctggag aaatcaccgc
   103501 ccgttcgcgt gcccgggacc gcggtgtata tgtcgcgtgc aatcaacgtc attccctttg
   103561 cgctgatgca taaccttaaa cataacaagg tattgcatga gcgggtgatt ctgttaactc
   103621 tgcgcaccga agacgcgcct tatgtccata acgtccgtcg ggtacagatt gaacaactgt
   103681 cgcccacgtt ctggcgcgtg gtggcaagtt atggttggcg agaaacgcca aacgtggaag
   103741 aagtattcca ccgctgcggt ctggaaggat taagttgccg gatgatggaa acctccttct
   103801 ttatgtcgca tgagtcgttg atcctcggca aacgcccgtg gtatttgcgt ctgcgcggca
   103861 agctgtactt gctgctgcaa cgtaatgcgc tgcgtgcgcc ggatcaattt gaaatcccac
   103921 caaacagggt tatcgaactg ggtactcagg ttgaaatcta acgccagacg cctcctttct
   103981 ccata
//
LOCUS       DAPOZO010000003        90965 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPOZO010000003 DAPOZO010000000
VERSION     DAPOZO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.003"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(228..1277)
                     /gene="yahK"
                     /locus_tag="ROI21_000206"
     CDS             complement(228..1277)
                     /gene="yahK"
                     /locus_tag="ROI21_000206"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDX5109190.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQVGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(1364..2320)
                     /locus_tag="ROI21_000207"
     CDS             complement(1364..2320)
                     /locus_tag="ROI21_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5109191.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(2317..3288)
                     /locus_tag="ROI21_000208"
     CDS             complement(2317..3288)
                     /locus_tag="ROI21_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5109192.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(3281..4765)
                     /locus_tag="ROI21_000209"
     CDS             complement(3281..4765)
                     /locus_tag="ROI21_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDX5109193.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGILVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSIPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(4814..5797)
                     /locus_tag="ROI21_000210"
     CDS             complement(4814..5797)
                     /locus_tag="ROI21_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX5109194.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMENLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(6051..6461)
                     /locus_tag="ROI21_000211"
     CDS             complement(6051..6461)
                     /locus_tag="ROI21_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDX5109195.1"
                     /translation="MTIKEKISQKYPHASFCTFGDSAVLADHLATLIATGVKTASCGS
                     LAGCIEDNAFPLIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGCYSPEMDVIFEEYALIDVV"
     gene            complement(6760..8142)
                     /locus_tag="ROI21_000212"
     CDS             complement(6760..8142)
                     /locus_tag="ROI21_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDX5109196.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENNQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(8152..9102)
                     /locus_tag="ROI21_000213"
     CDS             complement(8152..9102)
                     /locus_tag="ROI21_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDX5109197.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPQRIVEAPAIKALIQQGFVVIGAGGGGIPVVCSEAGNYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(9198..10616)
                     /locus_tag="ROI21_000214"
     CDS             complement(9198..10616)
                     /locus_tag="ROI21_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDX5109198.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(10616..12163)
                     /gene="fdrA"
                     /locus_tag="ROI21_000215"
     CDS             complement(10616..12163)
                     /gene="fdrA"
                     /locus_tag="ROI21_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDX5109199.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDISRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIDAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(12153..13016)
                     /locus_tag="ROI21_000216"
     CDS             complement(12153..13016)
                     /locus_tag="ROI21_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDX5109200.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITIGQHLHIEMSSCRRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKALHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYRGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(13056..13661)
                     /locus_tag="ROI21_000217"
     CDS             complement(13056..13661)
                     /locus_tag="ROI21_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDX5109201.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDQICSNPFLISCLTGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLNIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            13919..14416
                     /locus_tag="ROI21_000218"
     CDS             13919..14416
                     /locus_tag="ROI21_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDX5109202.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKTGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSTFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            14508..15440
                     /locus_tag="ROI21_000219"
     CDS             14508..15440
                     /locus_tag="ROI21_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5109203.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(15482..16570)
                     /gene="pdeL"
                     /locus_tag="ROI21_000220"
     CDS             complement(15482..16570)
                     /gene="pdeL"
                     /locus_tag="ROI21_000220"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HDX5109204.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(17447..19480)
                     /gene="betT"
                     /locus_tag="ROI21_000221"
     CDS             complement(17447..19480)
                     /gene="betT"
                     /locus_tag="ROI21_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDX5109205.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            19609..20196
                     /gene="betI"
                     /locus_tag="ROI21_000222"
     CDS             19609..20196
                     /gene="betI"
                     /locus_tag="ROI21_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDX5109206.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVDGNFDETQVS
                     NAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFHRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            20210..21682
                     /gene="betB"
                     /locus_tag="ROI21_000223"
     CDS             20210..21682
                     /gene="betB"
                     /locus_tag="ROI21_000223"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDX5109207.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFIPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            21696..23366
                     /gene="betA"
                     /locus_tag="ROI21_000224"
     CDS             21696..23366
                     /gene="betA"
                     /locus_tag="ROI21_000224"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDX5109208.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGSAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTARYFVANGMPVRVKK"
     gene            23577..24245
                     /locus_tag="ROI21_000225"
     CDS             23577..24245
                     /locus_tag="ROI21_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109209.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRIILFIITICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKSKYPAAICLILGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(24488..25183)
                     /locus_tag="ROI21_000226"
     CDS             complement(24488..25183)
                     /locus_tag="ROI21_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDX5109210.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNVVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(25176..26603)
                     /locus_tag="ROI21_000227"
     CDS             complement(25176..26603)
                     /locus_tag="ROI21_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDX5109211.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGSETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(26614..27332)
                     /locus_tag="ROI21_000228"
                     /pseudo
     CDS             complement(26614..27332)
                     /locus_tag="ROI21_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
     gene            complement(27860..28714)
                     /gene="rclR"
                     /locus_tag="ROI21_000229"
     CDS             complement(27860..28714)
                     /gene="rclR"
                     /locus_tag="ROI21_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDX5109212.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            28941..30266
                     /locus_tag="ROI21_000230"
     CDS             28941..30266
                     /locus_tag="ROI21_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDX5109213.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            30375..30611
                     /locus_tag="ROI21_000231"
     CDS             30375..30611
                     /locus_tag="ROI21_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX5109214.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            30623..31216
                     /gene="rclC"
                     /locus_tag="ROI21_000232"
     CDS             30623..31216
                     /gene="rclC"
                     /locus_tag="ROI21_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDX5109215.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKIEY"
     gene            complement(31376..32245)
                     /locus_tag="ROI21_000233"
     CDS             complement(31376..32245)
                     /locus_tag="ROI21_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDX5109216.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDTAKAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            32494..33351
                     /locus_tag="ROI21_000234"
     CDS             32494..33351
                     /locus_tag="ROI21_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5109217.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKMECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFAEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(33476..37726)
                     /gene="fdeC"
                     /locus_tag="ROI21_000235"
     CDS             complement(33476..37726)
                     /gene="fdeC"
                     /locus_tag="ROI21_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDX5109218.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHTVQPRLSMENTTVAADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKEFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLQKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKEQIKTEL
                     TFKPAGNIVTRTLKATKSQAQPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTLTAIWSTKDANDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASQAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKADIELISTVAGELEIAAAVKNSQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGTLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSDAQTITFVADKATATVSGIE
                     VIGNYALADGKAKQTYKVTVTDANNNLLKDSEVTLTASPANLALDPDGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAELAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            38292..39143
                     /locus_tag="ROI21_000236"
     CDS             38292..39143
                     /locus_tag="ROI21_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDX5109219.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRRERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            39170..40159
                     /locus_tag="ROI21_000237"
     CDS             39170..40159
                     /locus_tag="ROI21_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDX5109220.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGKLAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(40190..41083)
                     /locus_tag="ROI21_000238"
     CDS             complement(40190..41083)
                     /locus_tag="ROI21_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5109221.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(41283..42209)
                     /locus_tag="ROI21_000239"
     CDS             complement(41283..42209)
                     /locus_tag="ROI21_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5109222.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES"
     gene            42366..43286
                     /locus_tag="ROI21_000240"
     CDS             42366..43286
                     /locus_tag="ROI21_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX5109223.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALEKLIPFYKTHL"
     gene            43460..44602
                     /locus_tag="ROI21_000241"
     CDS             43460..44602
                     /locus_tag="ROI21_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDX5109224.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKRFAIGYRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            45075..45176
                     /locus_tag="ROI21_000242"
     CDS             45075..45176
                     /locus_tag="ROI21_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109225.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            45531..45794
                     /locus_tag="ROI21_000243"
     CDS             45531..45794
                     /locus_tag="ROI21_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDX5109226.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            45794..45934
                     /gene="ykgO"
                     /locus_tag="ROI21_000244"
     CDS             45794..45934
                     /gene="ykgO"
                     /locus_tag="ROI21_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDX5109227.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(46004..46194)
                     /locus_tag="ROI21_000245"
                     /pseudo
     CDS             complement(46004..46194)
                     /locus_tag="ROI21_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            47018..47560
                     /gene="ecpR"
                     /locus_tag="ROI21_000246"
                     /gene_synonym="matA"
     CDS             47018..47560
                     /gene="ecpR"
                     /locus_tag="ROI21_000246"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDX5109228.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVP"
     gene            47635..48222
                     /gene="ecpA"
                     /locus_tag="ROI21_000247"
                     /gene_synonym="matB"
     CDS             47635..48222
                     /gene="ecpA"
                     /locus_tag="ROI21_000247"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDX5109229.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            48280..48948
                     /gene="ecpB"
                     /locus_tag="ROI21_000248"
                     /gene_synonym="yagY"
     CDS             48280..48948
                     /gene="ecpB"
                     /locus_tag="ROI21_000248"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDX5109230.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYRFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            48974..51499
                     /gene="ecpC"
                     /locus_tag="ROI21_000249"
                     /gene_synonym="yagX"
     CDS             48974..51499
                     /gene="ecpC"
                     /locus_tag="ROI21_000249"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDX5109231.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            51489..53132
                     /gene="ecpD"
                     /locus_tag="ROI21_000250"
     CDS             51489..53132
                     /gene="ecpD"
                     /locus_tag="ROI21_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDX5109232.1"
                     /translation="MRVNLLIAMIIVALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRSGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            53101..53811
                     /gene="ecpE"
                     /locus_tag="ROI21_000251"
     CDS             53101..53811
                     /gene="ecpE"
                     /locus_tag="ROI21_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDX5109233.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVIDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            54125..54454
                     /locus_tag="ROI21_000252"
     CDS             54125..54454
                     /locus_tag="ROI21_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDX5109234.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(54703..55317)
                     /locus_tag="ROI21_000253"
     CDS             complement(54703..55317)
                     /locus_tag="ROI21_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDX5109235.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            55724..56413
                     /gene="paoA"
                     /locus_tag="ROI21_000254"
     CDS             55724..56413
                     /gene="paoA"
                     /locus_tag="ROI21_000254"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HDX5109236.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDFSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLDVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPENLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            56410..57366
                     /gene="paoB"
                     /locus_tag="ROI21_000255"
     CDS             56410..57366
                     /gene="paoB"
                     /locus_tag="ROI21_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HDX5109237.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVWNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRARCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPNGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTTENTFKLLLAKRTLASVLAEARAQA"
     gene            57363..59561
                     /gene="paoC"
                     /locus_tag="ROI21_000256"
     CDS             57363..59561
                     /gene="paoC"
                     /locus_tag="ROI21_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HDX5109238.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGCLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSEFLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAMLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            59571..60524
                     /gene="paoD"
                     /locus_tag="ROI21_000257"
     CDS             59571..60524
                     /gene="paoD"
                     /locus_tag="ROI21_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HDX5109239.1"
                     /translation="MSYSLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEVAAVRLRQEDSCLPPSS"
     gene            complement(60703..61830)
                     /locus_tag="ROI21_000258"
     CDS             complement(60703..61830)
                     /locus_tag="ROI21_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5109240.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALASSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATAVAAPLGSYLGATVGWRGAFLCLVPIA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSSILLIIGVAGFIGTLVVSAFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLAIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(61972..63030)
                     /locus_tag="ROI21_000259"
     CDS             complement(61972..63030)
                     /locus_tag="ROI21_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX5109241.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDVAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGKDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            63295..64179
                     /locus_tag="ROI21_000260"
     CDS             63295..64179
                     /locus_tag="ROI21_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5109242.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAQRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
     gene            complement(64613..65533)
                     /locus_tag="ROI21_000261"
     CDS             complement(64613..65533)
                     /locus_tag="ROI21_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDX5109243.1"
                     /translation="MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPR
                     LSVDIDLAYKSFADRDTDLAAINDALKRITASLNGRPGITAIRQENKADEKRIIVNTA
                     DAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
                     PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSG
                     MTQQETSLESLLSVTTLLPQALKSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAI
                     RWKQRNLAVLKDKNPAKYVAAVNKLERVLA"
     gene            complement(65520..66302)
                     /locus_tag="ROI21_000262"
     CDS             complement(65520..66302)
                     /locus_tag="ROI21_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDX5109244.1"
                     /translation="MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRR
                     IAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLTSLALQGHAHYTEQGKPQVWLAL
                     PAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELVAYEIA
                     CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQFI
                     DQSGIETGTGKRQIIPGGWLEPDYQITVPRAFKPEGVEDGSA"
     gene            66618..69827
                     /locus_tag="ROI21_000263"
     CDS             66618..69827
                     /locus_tag="ROI21_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HDX5109245.1"
                     /translation="MTYQAIFTGWDDLTIEDLLVAYRKAKADSFFENTFPVAIKFAEY
                     EQELLENLQKLLDLLQSEDGFSSNKKLIGKFRLLPKKLTTKKKHESQNGHVHFSNPKR
                     AADHLFNNFDLIPEFRIIGDFPVDSHIISALWINMVGHKFDASLDNCCYGARLKRIRN
                     DELFSNEQDNPFHISAVGSFSPYFQPYQKWRGDGLKAIRDELEKDRDIIAASLDLKSY
                     YHFIDPLAITSDDLYNTLNIKLTEDEKAFTAQLAVFLKHWSDGAAAFGKKIAYKTPVI
                     NGGLVIGLTASRIISNILLHHWDKLVIEKLSPIHYGRYVDDMFLVIRDTGTITNNHEF
                     MLLLQDRLGNDCVYLKNEQKQIWQIQQGEHFQGKTTIQLQSDKQKLFVLQGRAGIDLL
                     DSIEKEIYELSSEHRLMPSPDQLEHSTAAKVLSAAGSVGENADTLRRADGLTIRRLGW
                     SLQLRYVETLARDLPPSEWKEQREEFYQFAYNHILRADNLFAHFSYLPRLLGFAISMN
                     EWQHAEKIVLKAYESINLLASVITSGKEVNINGCKTRAVNDLWRCIKGTLSWLFVDAA
                     TRYYSPDRLFLDKRSKKEECLADTFFNHISQSLTNLKDLLDLRFDSADFYLKAPLVAR
                     ADLAKEPYKQIVKSQSAEKLVNQRDSKKEVKILKLMSDSSLIDIDVIKLFLKSTKNTR
                     LEKVAKGNRKNESYLPYIFPTRPLTPAEISELAPECVGLPSTSDKKPDERPSTIWAKY
                     TQALRGVWIKPTLLASEQDSDEATKKARPKKFIHIGTDRKHKVVVALTSIKTEEDDWA
                     KMACNKSNLSRSRYQRISELVNATLKLSPKPDYVLFPELSIPLRWVNSIADRLSSAGI
                     SLIAGTEYRHLDDNQLKSEAVLVLSDNRLGYPASVKIWQPKLEPAVGEDEALFSIYGK
                     SWDSTLNVKQRKPVYIHHGVNFGVMICSELQNSKARIRFQGALDALMVLSWNKDLDTF
                     ASLIESAALDIHAYTILVNNRKYGDSRVRSPAKEPFMRDIARVKGGDNDFVVAATLDI
                     DSLRAFQSRAKRWPKGGDKFKPLPEGFQLAKNRKKLPPK"
     gene            69988..70128
                     /locus_tag="ROI21_000264"
     CDS             69988..70128
                     /locus_tag="ROI21_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDX5109246.1"
                     /translation="MTRQRLFLCCSHGYISMVGRAGASQDAPVSDKAGKANPARFHHP
                     RD"
     gene            70174..70644
                     /locus_tag="ROI21_000265"
     CDS             70174..70644
                     /locus_tag="ROI21_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109247.1"
                     /translation="MATILTTDSDLLDLLTVPFNATTDFTVLADYCARFAETLIETDD
                     PALRLALCGRLHAALALLQPTLHDPIPPHLTESLTVDTLPATPEHFISEPDSLCEYCL
                     ALLQLLLERPAEPKMEQTLTGLLCELVWYFTEGLKAPRWLRTADGVKFISEVAA"
     gene            70641..70743
                     /locus_tag="ROI21_000266"
                     /pseudo
     CDS             70641..70743
                     /locus_tag="ROI21_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773560.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            70835..70993
                     /locus_tag="ROI21_000267"
                     /pseudo
     CDS             70835..70993
                     /locus_tag="ROI21_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(71008..71083)
                     /locus_tag="ROI21_000268"
     tRNA            complement(71008..71083)
                     /locus_tag="ROI21_000268"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71048..71050),aa:Thr,seq:cgt)
     gene            complement(71198..72451)
                     /gene="proA"
                     /locus_tag="ROI21_000269"
     CDS             complement(71198..72451)
                     /gene="proA"
                     /locus_tag="ROI21_000269"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDX5109248.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCDLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADVAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(72463..73566)
                     /gene="proB"
                     /locus_tag="ROI21_000270"
     CDS             complement(72463..73566)
                     /gene="proB"
                     /locus_tag="ROI21_000270"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDX5109249.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            73854..74909
                     /gene="phoE"
                     /locus_tag="ROI21_000271"
     CDS             73854..74909
                     /gene="phoE"
                     /locus_tag="ROI21_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDX5109250.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(74948..75349)
                     /gene="crl"
                     /locus_tag="ROI21_000272"
     CDS             complement(74948..75349)
                     /gene="crl"
                     /locus_tag="ROI21_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDX5109251.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(75407..76651)
                     /gene="frsA"
                     /locus_tag="ROI21_000273"
     CDS             complement(75407..76651)
                     /gene="frsA"
                     /locus_tag="ROI21_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDX5109252.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(76743..77201)
                     /gene="gpt"
                     /locus_tag="ROI21_000274"
     CDS             complement(76743..77201)
                     /gene="gpt"
                     /locus_tag="ROI21_000274"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDX5109253.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            77462..78919
                     /gene="pepD"
                     /locus_tag="ROI21_000275"
     CDS             77462..78919
                     /gene="pepD"
                     /locus_tag="ROI21_000275"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDX5109254.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(78976..79328)
                     /gene="prfH"
                     /locus_tag="ROI21_000276"
                     /pseudo
     CDS             complement(78976..79328)
                     /gene="prfH"
                     /locus_tag="ROI21_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(79324..79530)
                     /locus_tag="ROI21_000277"
                     /pseudo
     CDS             complement(79324..79530)
                     /locus_tag="ROI21_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(79775..80227)
                     /locus_tag="ROI21_000278"
     CDS             complement(79775..80227)
                     /locus_tag="ROI21_000278"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDX5109255.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            complement(80237..80635)
                     /gene="yafO"
                     /locus_tag="ROI21_000279"
     CDS             complement(80237..80635)
                     /gene="yafO"
                     /locus_tag="ROI21_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HDX5109256.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(80638..80931)
                     /gene="yafN"
                     /locus_tag="ROI21_000280"
     CDS             complement(80638..80931)
                     /gene="yafN"
                     /locus_tag="ROI21_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HDX5109257.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(80983..82038)
                     /gene="dinB"
                     /locus_tag="ROI21_000281"
     CDS             complement(80983..82038)
                     /gene="dinB"
                     /locus_tag="ROI21_000281"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDX5109258.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(82109..82894)
                     /gene="lafU"
                     /locus_tag="ROI21_000282"
     CDS             complement(82109..82894)
                     /gene="lafU"
                     /locus_tag="ROI21_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDX5109259.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            82866..84578
                     /locus_tag="ROI21_000283"
                     /pseudo
     CDS             82866..84578
                     /locus_tag="ROI21_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            84657..85010
                     /locus_tag="ROI21_000284"
     CDS             84657..85010
                     /locus_tag="ROI21_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDX5109260.1"
                     /translation="MSQASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEQKRLTLRVPHHIDSRLTIVARQHSISKN
                     QLIVDVLEREFSAQM"
     gene            complement(85067..85816)
                     /locus_tag="ROI21_000285"
     CDS             complement(85067..85816)
                     /locus_tag="ROI21_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDX5109261.1"
                     /translation="MSSFFHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRKALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            86117..86857
                     /locus_tag="ROI21_000286"
     CDS             86117..86857
                     /locus_tag="ROI21_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDX5109262.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(86828..87595)
                     /locus_tag="ROI21_000287"
     CDS             complement(86828..87595)
                     /locus_tag="ROI21_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDX5109263.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(87711..88289)
                     /gene="lpcA"
                     /locus_tag="ROI21_000288"
     CDS             complement(87711..88289)
                     /gene="lpcA"
                     /locus_tag="ROI21_000288"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDX5109264.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            88529..>90965
                     /gene="fadE"
                     /locus_tag="ROI21_000289"
     CDS             88529..>90965
                     /gene="fadE"
                     /locus_tag="ROI21_000289"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDX5109265.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVE"
BASE COUNT        23177 a        23458 c        22149 g        22181 t
ORIGIN      
        1 ataaaaataa acgtcgcaga taataaagcc ctgattaaac caggcactat cagaaatcgc
       61 tcataactaa cgtgtgaagt attgtgtact ggagggagtt aatttaacaa tttgaattta
      121 cgttataggt tttactgcta ctttgatatt atttcgctct ccttaactaa aaagttaagt
      181 ggagagcgaa gttaatatgt taaaccacag ggtatttatt aattttttca gtctgttagt
      241 gtgcgattat cgataacaaa acgatatttc acatctcctc gcagcattcg ctcataggct
      301 tcattaattt gatcggcccg aatcatctct atatcagcca cgatgccatg ttcggcgcaa
      361 aaatcgagca tctcctgagt ttctggaatg ccgccaatca tagaaccggc tatcgcacgg
      421 cgtttcatga tcaggttgaa aacttccggc gatttatgcg gtgtcgcagg cgcaccaacc
      481 agcgtcatgg tgccatcacg cttcagcaag gtggtaaaat cgtcgagatt atgtggcgca
      541 gctactgtat tcaaaatgaa atcgaaactc ttcacatgag ccgccatctc atcggcattg
      601 cgtgagttaa caacttcatc ggcccccagg gcttttgccg cttcgcgttt tgactcagaa
      661 gtggtaaatg ccaccacatg cgcccccatc gcgtgggcca gcttaatccc catatgtccc
      721 agaccgccga tgccgaccac gcccactttt ttacccggac cgacctgcca gtgacgtagc
      781 ggcgaatacg tggtgatccc tgcacacaac aaaggtgcca ccgccgccag ctgctcttgc
      841 gggtgacgaa tacgcagaac atatcgctca tgaacgacga tctgttgtga gtagccgccc
      901 agagtatggc ccggttcgtc cggcgtcggc gagttatagg tgccggtcat gtgatcacag
      961 tagttttcca gcccgtcttc acactcttcg caatgtttac aactgtcgac aatacagccg
     1021 acaccgacca gatcgcccgg cgcatatttt tctacctgat caccaacggc taccacgcgc
     1081 cctacaattt catggcccgg cacgcaggga taaaccgtcc ccgcccactc ggaacggacc
     1141 tggtggagat cggaatggca gacgccacag taagcgattt caattttgac atcatgcggt
     1201 cccggttcac gccgggtgat atccatcggt tcaagtggtt gtttagcgga atatgcacca
     1261 acagctttga tcttcattgt gtttactcct gattagctat gtgtatttgg cgatgcctga
     1321 tatgtcatcg cggcaaaacg cgtccattga atatagccaa tatttaaacc acccgatcca
     1381 gccccaacat gccgcgagcc tgaatgaaac agagcagcaa cgttccccat aaggccatcg
     1441 tcaggtaggg gcttataccg agaatattga agccgctttc aataatttgc agaagaaaca
     1501 gcgccagcac cataccaatt atccgcccgc tgccgccgtc cggattaacc ccgccgagta
     1561 ctgccgcgag gattgacacc agcaagtacg attcgccata agaggcctta gccgagttga
     1621 gtttcgacat catcaaaaat gccgctaccg cacaaagcaa ggcggaaatg acataaaccc
     1681 aaatcagcac ccgacgagtg ttaatgccgg agtaaagtgt tgctcgttcg ttagaaccaa
     1741 tcagataaat ggatttaccg agcggcgttt tctccagcaa tatccacagc cccagcgcca
     1801 ctgcggcaaa caaccacatc ggtagcggaa taccgaacca ctgtgcatga ttcaaccaca
     1861 gcacccatga gggataattg gcaatggcgc taccgcctgt caccagaata tttacccctt
     1921 tgagcaacgt catcatcccg agtgttgcaa gaattggcga cactcgaata ccagcaatca
     1981 atacgccgtt acataaaccg attatcaccg ccgcgccagc gcccgccagc agcgtagcga
     2041 caaccgtagc aatacccggt ggatactgag tcgccaccca cgccatcacc agtgaacagg
     2101 cgttagcagt ggctataatt gacaagttaa tgccaccgca aagcatggtc accgccatag
     2161 ccaacgccag aatgcccagc acgggcattt gcgaagcgac ggactggaaa ttacttatcg
     2221 accagaaaat ctccggcata gtaatgctga acgcggcaac agttagcacc agcaggccga
     2281 tcaagtaaaa ctcaacgtta ttacgccacg attttttcat agcagactcc ggttctgatg
     2341 ctgactccac gccgtggcgc taatgctggc aacgatgatg atgccggtga tcaatgtttg
     2401 ccagtaagac gagactccca ataaatttag cccgttttgc atcactgcca acaacaccac
     2461 gccgagcaaa gtaccggtta acgtgccgcg cccgccgagc aaactagtgc cgccaagcac
     2521 caccgcagcc agtactgtca gctcataacc cagaagagaa tcgggggcga cagtcatcac
     2581 cgtccacgac tgcactacac ccgcagcgcc agacatcaat cccatatagc cgtagacgaa
     2641 aagttgcagt ttcagcacgc taaagccgat gcgactggcg gattcacggt tcccgccaag
     2701 ggcataaatt ttgcgcccta cactggtgaa attcatgata aacgctgtca gcagcaccac
     2761 cgtaatcatg gcgatcagcg gcagaccaag gccatagtca tagccgtcag catcggtgta
     2821 cttgaacaac attaccccct gctcaaacca cggcggaaag tcgtacagcc acacaccttt
     2881 actcaaccat aacagcaggc catagaaaat attcagcgtc gaaatggtga tgatgataga
     2941 cggcacccgt aggcgattaa ccagtaaggc gttaattaag ccaagtaaaa tgccgatgcc
     3001 gcccgccagg gcaaacgcaa cagcaaagtt gccaccaatt ttttgcagca gcagcaccat
     3061 gccgtattgc gcaatgatgg tcattgccgg aaacgaaata tcaatcccac cggaaattaa
     3121 caccacaaac aaaccacagg cgagaatagt caacatggca tagttattgg cgagatcgta
     3181 caaattaccg aatgtcagaa actcgtcgct gcgccaggca aggccaacaa acagcaccac
     3241 aatcagtaat gccagccaga attcatggcg ttttttcagt tcagccattt accacctccg
     3301 caatcctggc ctgagaactg ctgtcaggca ggaagctatg ggtgatctga cctttttgca
     3361 ttaccaaaat ccggtggctt tgataccacg cttcttcaat ttcatcgcag atcatcaata
     3421 ccgcaatgcc atgagccgcc agatcgctga taatgccgta tattcctgct ttgttggcga
     3481 tatcaacacc gacagtcggg ctatcgagaa tcaacagtcg tggcccaatc gccagccatt
     3541 tggcgatcga tactcgctgc gcgttacccc cagaaagcgt attcaccggg agatgtgggt
     3601 cagagacttt gatggtcaat gccttgatta gcctgtccac caggtcacgc actttagctt
     3661 cactcagaaa gccgaagcga tcttttacct tgtgaaaaac agtgctaatg atgttgtctt
     3721 caatagattg cgccatcacc aaaccgcgcg acatgcgatc ttccgaaaca taaccaatac
     3781 cgtggcggat ggcgtcctgg ttactatgca gtgtaaccag ctggccgttg atgaggatct
     3841 cgccagcatc cggtcgggtc ataccaaaca gactcagaca taattccgtg cgccccgcac
     3901 caagcaggcc aacaatcgac accacttcgc ccgcttccac ccgcaagtta atattttgat
     3961 attccccatg acgacttaaa ttacgcacct ccagcactgg gatgctggat tttccctgcc
     4021 acaactctcg cacctgatat tcaaactcct ggccggtcat caggaaaccg agttgttttg
     4081 tggtcatctc tgcagccgga aaggtcccga ccagctcacc atctttcagc actgaaatac
     4141 ggtcggaaac ttccatcact tcttccagac gatgactgac gaagaccacg cagatcccgc
     4201 gttcccgcaa ttgatgcacc acctgtaata acccttgcac ttcctgatgg gtcagcgaag
     4261 cggttggttc atccatcacg atcaaccgtg cgtcctgcgc cagtgcacgg caaattgcca
     4321 ccagctgaca gcgggcaatt gaaagctcag aaacgagcgt atccagtggc aatgtgacgt
     4381 taatactggt cattgccgcc tgcgccacct cacgcagacg acggcggtta acaaacagac
     4441 cgtggtgata gtgattcacg gcgatattct cccagacact caggttagga aataaagaga
     4501 ggtcctgata aatcaccaga atcccctgcg ccaccgaagc ggcgggtgtc agctttgacc
     4561 agtttttccc accaagcgta atttccgccc cctcatccgg gcgataaaca ccggacataa
     4621 ttttaatcaa cgtcgattta ccacagccgt tttgcccggc aaggcagtgc acctcacccg
     4681 acataaagtc gagagaaaca tcgcgcagcg ctcgcgtggc atgaaacgtt ttgttgatat
     4741 gacgaaggga aaggaaggtt tccatagcat cccctggcaa taagaatcgg ggctttcgcc
     4801 ccgaagaaat ggattaataa agtgagtcga tattgtcttt gttgaccagt aataccttat
     4861 ggaaacggat aattcgctta tccatatcta cgtcggcctt accgagattc tccatttcta
     4921 atccaggttt gatctcttca cccttcagca acgtgctggc gacggccgcg agtgcgtaac
     4981 ctgcagaggc cggatcgtaa gtaatccctt cggtgatatc accactttta atcagtgatg
     5041 ccgcctgtga agggatcatc atgccataga ctgcgacttt atttttcgcc cgtttctctt
     5101 tcactgcacg tcccgcgcca atcggaccgt ttgaaccaaa ggagacaacc gctttcaagt
     5161 caggataggt tttcatgagg tccagtgtgg tacgacgtga gtcatccaca ctctcggcaa
     5221 ccggcatgcg gcgggtaact tcatgcatat ccgggtaatg ctctttctgg tatttcacca
     5281 gcaagtcagc ccataagtta tgctgcggca cggtcaaact acccacgtaa atcacataac
     5341 cgcccttgcc acccatgcgt ttcgccatat gctcaacata ttcagcggca aatttttcgt
     5401 tatcaatgat ttcgatatcc cagttagcac ttggctgacc gggggattcg ttggtcagaa
     5461 ccacaattcc ggcatctcgc gcttttttga ataccggttc cagcacgttg gcatcgtttg
     5521 gcacaattgt aattgcatta accttacggg cgattaaatc ctcaataatt ttaacttgtt
     5581 gcggagcatc agtacttgaa ggccccacct gtgacgcatt aacaccaaag gctttacccg
     5641 cctcaaccac accttcgccc atgcgattaa accacggcat accatcgact ttagagatat
     5701 tcaccacgac tttttccgct gcctggagcg gcgcagaaat tagcgcagcg cctaataaca
     5761 gcgaagacac catattgata acaaaacgtt tattcatcat ttcagcctca ttgcagggta
     5821 gtcaggaagt gttcgcccaa attagcgcgc tggagagaat aagaattttc gaatattttt
     5881 atgcagaata aacagcccta tttcaggcag ctattaaaaa tttggttata aatatggaca
     5941 aaacgggcag taacaataag tgtgacaatc atcacgtaaa aaacagcaaa ataaacaaaa
     6001 ttagcgatgc tttatgtttg tgagcttatt tcgcgtagaa aaatagttat ctacacaacg
     6061 tcaataaggg catattcttc aaagataacg tccatttctg gtgaatagca tccttctgcc
     6121 tggaaaaaac gacggtgttc attacgccaa tattcaagac tgaggtcacc ttcaccttcc
     6181 ttacgggcaa gctctgcggt gacatcagaa aagcgcagta aatgcaggcc aatcactctg
     6241 ataacacaga caggctcacc acggctattt tctacaatct tatactcacc aatcagcgga
     6301 aatgcgttgt cttcaataca tcccgccagc gaaccacagg aagctgtttt aacaccagtc
     6361 gcaatcaacg ttgcgaggtg atcggccaac actgctgaat cgccaaaagt acaaaaggaa
     6421 gcatggggat atttttgtga gattttttct tttatggtca tgagaatatt ttcgcgatta
     6481 cggaaaaaca aatcacatct taattactgt tgataaccat cagcaagaag atcattggtt
     6541 tttcgatgct taatcttttt tatccaaagc aggtcacatt tatggcttga taaatacctt
     6601 tgttaaaact cacttttgtg tatttctctc aaataatccc ctggaaatta tgccgggaga
     6661 ttataaagtt caggatcatt cattagcaaa ccattgctgc cccactccct ggggcttaaa
     6721 tctcttacta ttcaccgttg caggcgtttc aaatgcgtat caacctgcca cactccccca
     6781 caccagttgc cctttatgga acgtcgccgt tctcggcgag atacgcgcca ccgcctcggc
     6841 ggaacaggag gcgtccacca gcacaaagct ggcatcatcc tgcgctttgg gccacacgcg
     6901 ctcgcctttt tcgttgagcg gcaatacatc gccagtggct aaaaacagcg agcgggagag
     6961 gttctgttcg ttaggacgaa tatagagctg cgcgtacaga ttggcttttt ccagcatgtc
     7021 gcccaggccg taaggcgacc agtggtcgat aacgctgtcg gtgccggtca tcacttttac
     7081 gcctttgtcg tgcaactgtt tgagcggcat atgcaacgtg ccaatcggca ccgtcgaggc
     7141 gatagaaatt tgctgcgccg ccatccggtt cgccagttca tctacctgtt gttcgttgag
     7201 cgttgccaac gcaaaggcgt ggctgatggt tagcttgcct ttcagttgcg gcgttttctc
     7261 taccgtttca accatataat tgatggctgc cacgcctgcc ggagtggttt cgtgcaggtg
     7321 aatatcgacg cctttgtcgt agtccagcgc aatctggaac atggtgtcga gggatttttc
     7381 catcgcgcca tcgacactgg tcgggtccag cccgccgacg taatgcgccc ccgcctgcat
     7441 cgcttcgcgc attaagggtt ccgatttcga cagcagcaaa ccgtgctgcg ggaaggcgac
     7501 gatttcacac tcgaagcccg cctgacgtcg cgccagtacc gcctgcaaat tttgcagatt
     7561 ttttaggccg gaaaccggtt cgatattgca gtgactgcgg gcaatggtgg tgcctttcga
     7621 ttgcaataaa tcgatcagtt tttccgcccg ttcctgagtg tacggttgca actccggcag
     7681 cattttctgc tcaagtttga tcatgtcctg gatggtggtg cctgccggac gattgagcga
     7741 gcgccacggc ccgccgtaaa aggttttgtc gagatgaata tgcatgtcgc gggtagtggg
     7801 cagcatcagc ttaccgccag cgtcatagtg cggcagcgtg gcgtccggat gctgattgtt
     7861 ctcgcgcagt gcaacaattt tgccgttctg aatttccaca gtctggcgcg ccgtgcgggt
     7921 ctgcaccgcc acgccatttt cgtagtcaaa accggtttcc agcaacacgt tgtcgagata
     7981 gtagtgcggg tcagtgattt tcatggtgtt attcgcgtcg cagtgggttg tgccagagac
     8041 tgccgcatgg gcgagcggca cagaggtacc aaacagcgcg gcggcggtga ccatcttacc
     8101 gctctggctc agaaactcac ggcggctatt gctttctttc atcttacgtc cttacgtttt
     8161 aataatatgg gtgcccgttt caccgcgcag cgccgcaggc aggcattccg gcgtggtgat
     8221 aatcacttct ttgccgccct gttccaggaa cgtcaggctg gcgatgattt ttggcaacat
     8281 gctgccgggt gggaaatgcc cttcctgcat atagcgggtc atggtggcaa tatccacccg
     8341 atccagcgcc tgttgctgcg gtttgccaaa gtgaatacac actttttcga cgccagtggt
     8401 aatcaccaga atgtcggcgt gaatttcacg ggccaatagc gcggtagaga gatctttgtc
     8461 gataaccgcg tccacgcttt ggtaattccc cgcttcactg cacaccaccg gaattccgcc
     8521 accgcccgcg ccaatcacca caaagccttg ttggatcagc gccttaatgg caggtgcttc
     8581 tacaatacgt tgcggttccg gcgaggcgac cacgcggcga tagccccgtc cggcatcttc
     8641 aacaaaacgc cagtcagggt ttgccttttg taattcgtca cgctggcttt cactaaagaa
     8701 tgcgccgatg ggcttggtgg gatgggcaaa ccccggatcg tttttatcca cttccacctg
     8761 agtcaccacg gtgacggctt ttttctcgcc gtggcgggcc agccggttgt tcagcgcctg
     8821 ttggatcaga tagccaatgc cgccttgcgt atccgccaca cagttcgcca gcggcgttaa
     8881 gggcagccct tcgcgctcgt gggctatctc cgcgcggcgt aaatccagac cgacctgcgg
     8941 cccgttgccg tgggtcagca caatgtcgta atcggaagcc agcatttcca gcaccgtatc
     9001 ggcgacggct ttcaccgcct ccgcctgatg ctcaatcgac tggctggcgt tatctttgat
     9061 aatgctgttg ccaccaatgg caacgaccac aagctctttc atgttgttat gtccgctaca
     9121 gagggtgatg gtgttatgag agtgaggttg cccctctccc cagaagggag aagggatcgt
     9181 aaggtgcagt tggagggtca gacgccgtaa cgttcgcacc agccaagaat ggctttctcg
     9241 aagcaggcga atggtggatg cacaatgccc gcgccaatca tgccgacgcc cgcatcttta
     9301 tgggcgatgg cggtgttgat caccggcaga atgccgctac tgcccacgcg ggttatgtcg
     9361 attgccgacg gcacgcccat aaaaccgagc agcggaatgg tgacgttggg attttcgccg
     9421 agagtgattt cgcgcatctg gcgggagaaa tcgatggcct cttccaccgt accgcccacc
     9481 agcgcgacga ttgcaggcgc ggtcgccata gcaaatccac caatgccgta ggtttcggtg
     9541 atggcgctgt cgccaatatc caacccagaa tcttccggct tataaccggc aaacatcggg
     9601 ccgatgacct gctgcgccgg accggtaaac cattgccccg gtaatccgct gacccgcagg
     9661 ccgaactcga cgccgttacg cgccatggtg gtcaccacag tgctgtattc gatgccgtgc
     9721 gccgcatcca tcgccgcttt acacatcgcc atccacgtcg ggccggagaa gtagtcactg
     9781 ctggcgacaa actcaaacac ttcgcgctgt tgctcgacgg agtagcccgc ctgaataatc
     9841 cccggcgtca gcgcctgaat cagcagcgtc gtcccggcgt tattgcggtt atggcactca
     9901 tcgcccatat gcagcgcctg cgccagcatt aagcgcagat cgatctcgcc gataattttc
     9961 atcgcatcgc gcagcattgg cccctgcaca tcacgcatcc agttgagtcg gtcgatcacg
    10021 ctctggtcgt tagcgcccat acgcaaaatc tttgccatct gctcgctcat attggtgtaa
    10081 gcgatgttgc cgtaggtttt gtttttcacg atgtgcataa acatcgaggc cgaggtaaca
    10141 cccgccatcg atcccacgca gtcatgttcg tggcacggcg agaaggtgat ctccccggag
    10201 gccgccagtt cagccgcctc atcgagatct ttcgccagcc cttcgaacac cagcgctccg
    10261 gtgaccgcgc ctttcatcgc gccacacatt ttttcccagg tgatcggcgg tccggcgtga
    10321 agaatggttt tcggcgtcat acccggcacc acgttaatcg cctgatcaaa accaatcagc
    10381 accggatgcg actggataat gcgctccaaa gcctgctggt tagcggcggc gattttgtct
    10441 gccagcggcg aatcggcaat gttatccagc gcctgcacca cctgcatatt gccctgcccc
    10501 ggcggcgtcc agtcgagttg agtcacttct acatgctgct ttttcaggtc atcgctaaac
    10561 atggcgatgc cgacgttaat aacgttcaac ggttggctaa acagtgactg gctcatcagg
    10621 cttcctcccc tttgcagata aattcacgcg ccagcaatcc ggtattggtg ctgctgcttg
    10681 ccagaattac cccagcatca agcagcatct ggctttgttg ttctaatgat ggcgtatcaa
    10741 gatcggtacc cagcacatag gcgagaatga tcaactcacg tccttcggca gcggcgatcg
    10801 ctttcgcttc tttgatcgca tcgatggtgg agccgaccgg atcttcatgc gatccaaatc
    10861 cgagcacaaa atccatcacg atcaccgcca cttctggatc gcgcgcttct tcgatcaagc
    10921 gactgatgcg gttggtcggg tcaatcattg gatgcggctt gccgttggtg aagtcgtcat
    10981 caccaaagtc gaggaaggtg tgtttgatgc tgcggctgat atcttgcagg cggaattccg
    11041 gatcgggctg aatgttgctg tagacatcgc cgtgtttttc catcaccgcg aacatcgttt
    11101 cgtcgcacag cgtgccgccg cagaacaggc cacgaatgta tttctgttgc ggttgcaggc
    11161 gagcgcgcac gtcagcaatc aatggctggt tcagggtatg caggtcgaga tgttcctgtt
    11221 tcacgccgga gagcattacc gctttcagcg ccgcctcttt actgccgcgg gcaaactgta
    11281 gcccctgctc atccaccggc gtttcgccac gaccgaggaa gcaaaccacc actggcttgc
    11341 ggcaggcacg cgcgcgttcc agcactttgc gggccaccgc aggcgcaggc ggtttggaga
    11401 taagcacaat gatttcagtt tgcggatcgt tttccagcat cccgatggcg tcgagcatca
    11461 tcaggccgcc gattttctcg ctcaggtcgc gcccgccggt gccgatcagt tgcgaaacgc
    11521 cgccgccaaa ttcatggata cggacgctca gttcctggct accggtgccg gatgcgccaa
    11581 caataccgat gttgccgcga cgcacggcgt taccaaagca gagcgccgcg ccgttgataa
    11641 tcgccgtacc gcagtctggc cccatcatca gcagcccttt ttcgtgggcc aactgtttga
    11701 gcgccagttc atcttcaact gagacgttat cggaaaacag catcacgtta agatcgtttt
    11761 gcagcgcctg acgcgcttcg cgagcggcaa acagaccgtt gaccgaaatc accgccaggt
    11821 tactttccgg gatatgcttt ttggcgctgg caatagtggc gtaacgcgcc tcgtgcgagc
    11881 cactttgcgc tttggtgttg aacagttctt caatctccac cagcaactgc tcgttgtcca
    11941 cacccgattt accattgatg acaatcatca ggtcgccgtt tttcgcctgc tccagctccg
    12001 gcgtcagcag tcctaaattc ttcagcacgc ctttgttcat ttcggtcgcc atcgccacaa
    12061 atgcctgctc gacgccgtcg agtttatttg cacgcgtgga gatagacatc agcgagacag
    12121 aatcaaaata ggtattcggt ttaatgacta ttttaactga cataattccc tccgtaggtt
    12181 tggctcagcg cacaaccatc ggccatgcca tacagcatgt cgcagccgga actggagcca
    12241 atatgtttaa ttttttctat tgcctgagtt gcgttcccag ggatgtcata aataatgtta
    12301 tgaataaaac gatgaatatt ttcccggcag cgttgttgta atgcggcttc cagcgttatg
    12361 gcacttaata acgtggtatt atttctgccg cgttgcagac cgcgataaaa ttcctctttg
    12421 tatttttctg ccggatgccc ggaaataaat aaaataagcg ccagacctgt taaataatca
    12481 tcggctgatg gcgtaaggcc aatccctaag cccatcatac tggcgactgc tgaggcgata
    12541 ttttgtttat cgtttactgc ctgaataact gcccgacgtt taatatgtag tgctttattt
    12601 aacgcctgat aaaacggatt atcgccgcga taattaaaca gggtttcatt ttcccccagg
    12661 tgctgatgaa taatgtcatg ccactgttgc caggcgataa ggtgaaaatt cacagcggcc
    12721 atttgcaagg ttggggacag ccaacgccga caactactca tctctatatg aagatgttga
    12781 ccaatcgtaa tgccttgatc gtcaaactga accttatcac catgccggaa cagaccatca
    12841 aagtgagtga gtgccaaccg acaactgttt ggcgcattgt cgtactcttc acaaagcagc
    12901 gtcagcaact gctggcgggc cggaagtgcg atatttaccg ctctggcaaa gacctgttca
    12961 acctgtcctt gcccccgctg cgccagaaaa tcagcatccg cagttagcgc ccacacacac
    13021 cgtctttctg gtgtgttgat agcaagcctc ccggtttatg cacctgcggc aatcagtaac
    13081 tgcgcaatct cttcaaagcc ccgttctcgc gccagttcca gcggcgtttt gccatattta
    13141 tccgtcatgt gcgggctggc accgtgttcc agcaataact ggacaatcgc ctgctgttta
    13201 atcccgccat cattaagcac aattgcttcc agtagcggcg tccagccaac atggttggtc
    13261 tggttaacgt taatctccgt atgcgccaaa agctctttta caatatttaa atggcctttt
    13321 tcacaggcag gcgtcaggcc gacaccgcca aaacgggtta cgcaattaag atcgggttta
    13381 gccggtaaaa taatccgtaa cagggttaaa tcgccggtca ggcagctaat taaaaaagga
    13441 tttgaacata tctgatcttg cttattaata tccgctccgg catcaattaa tgcctgaacg
    13501 caagcatatt gctgatataa acttgccagc gtaattgccg ttttcccctg acgatcgcag
    13561 gtattaatat cgacacccag cgcaaggcag gtttttactt tatcgatatc accctgttgc
    13621 gcagccaata aatagtcggc aggtagattt tttatagtca ttcacattcc cctgtttttt
    13681 gtggctgttt tcgatgaatt gagaatagca acgataatct gataaaagaa cccgcttaaa
    13741 tttaattcgc cgtatttcgc ctttttgttg aatgatgttc aacaatcttt tcgcagtgtg
    13801 cggtagtgca atatttgttt ctttgacccg ataccctttc actcaatcag gttttatttc
    13861 tctatagtct gcacatgtaa gagtagcttt cgtccatgtc taaccagagg ggaacaagat
    13921 gaacggactc acggcaacgg gtgtcaccgt cggtatttgt gcagggcttt ggcagttagt
    13981 gtcttcacat gtcgggctct ctcagggatg ggaattatta ggaaccattg gcttcgtcgc
    14041 cttttgcagt ttttatgccg caggcggcgg taaaacgggg tttatcagaa gcctggcggt
    14101 taattattcc gggatggtgt gggcgttttt cgccgcgctg gctgccggtt ggctggcctc
    14161 ggtgagtgga ttgtccacgt tctgggcgag cgtgattatg actgtaccct tctcggcggt
    14221 agtggtctgg caaggacgtt tcggtttatt gtcttttatt cccggaggct ttctcggcat
    14281 gacgctattt tttgccagcg ggatgaactg gacggtgaca ttactgggtt ttctggcggg
    14341 taactgcgtg gggataattt cagaatatgg cggacaaaaa ttaagcgaag cgacgactaa
    14401 acgcgacggg tattaaaagt tattcaaaaa tgattatcta tctttaaccg atacctctac
    14461 agtcatggct ggagaaattc atagtagata atggcgggaa tggtttaatg aactcaattt
    14521 ttaccgaaga gaatctgttg gcatttacta ccgctgcgcg ttttggcagc ttcagtaaag
    14581 ccgcagaaga gttgggttta accacttccg ccattagcta caccatcaag cgaatggaaa
    14641 cagggctgga tgtggtgctg ttcactcgca gtacccgaag cattgagtta acggagtccg
    14701 ggcgttattt tttccgcaaa gctaccgacc tgctgaatga ttttcacgcc atcaaacgca
    14761 gcatcgatac tatttcgcag ggtatagaag cacgggtgcg catttgtatt aaccaactgc
    14821 tttatacgcc caaacacacc gcacgcttat tacaggtact gaaaaagcag tttccaacct
    14881 gccagattac cgtgacgacc gaagtgtata acggcgtctg ggatgcgatt attaataatc
    14941 aggcgaatat tgctatcggc gcgcccgaca cactgctgga cggcggcggc attgattaca
    15001 cggagattgg cgcaattcgc tgggcttttg ctatcgcccc ggatcaccca ttagcctttg
    15061 tgccggagcc gatcgccgaa agccaactgc gtctctatcc taatatcatg gtggaggaca
    15121 ccgcgcatac gattaataaa aaagtgggct ggttgctgca tgggcaggag tcgattctgg
    15181 tgccagattt caacaccaaa tgtcagtgcc agattttagg tgaagggatt ggttttttgc
    15241 cggattacat ggtccgtgag gcgatggcgc aatccctgct ggttacccga caaatccata
    15301 atccgcgcca ggattcacgc atgttactcg caactcagca ttctgctacg ggtcaggtga
    15361 cgcagtggat taaaaaacaa ttttcgccaa atggcatatt aacggggatc tatcaggatt
    15421 tactgcatcg ggagaattaa tcgctgccaa cctcccgatg cgctgtgaat agcggtttac
    15481 atcaaccacc tgctttcatt acccattcag agataaattt attacccggt actggcggag
    15541 agtacaaata gccctgcaaa aagtgaacgc ctttgccgat cattaaatcc gcctgctcct
    15601 gggtttctac cccttccgcc acgatactca gaccaggctt acgcgccagt tcgacaatat
    15661 tgtccacaat atgaccggat atttcgtcca cgctcgccat ttgcacaaat gacttatcga
    15721 tcttaataaa atcgaccggg aacgcctgca agtaacgata ggtcgcataa cccgtaccaa
    15781 agtcatccag cgcaaaggta atgttgtgct gatgaaggct gtcaaatatc gctctggctt
    15841 ctggcgttac cggaataggg ttacgttccg ttagctcgag aaccagtttg attttatcgt
    15901 tacctaattt cttaaccagg ttcaggcatt ctttttcaaa tcccgcagcc aaaaaacaac
    15961 ccgccgagac gttgatgcca atatggaaat tgtccggcag caaatgtttt accggcatca
    16021 gaatatccgc agtctgtttc atcaactggc gagtcattat gacgataaga ccagatgact
    16081 ccgccagagg aataaactga tccggtggga taattcccgt ttgtggatgt tcccagcgga
    16141 caagcacctc acagcccgtc agtaccccag tctgcgcgca gaaaaccggt tggatccacg
    16201 gtttgaattc gtggttttcc aacgccagac tgatggcttc aggcgtgacg atatggtgat
    16261 agtgattatc attaatatat ttgtgactat tattacccgt ggcggatatg atctccggat
    16321 tgtactgaaa gaaaatatcc cgccagaaag taatatcgct ctgaatcccc agtttctcaa
    16381 agagctgttt cttttgatgt gaaatggtct ttgcactgcg atttctgtac tgtgatattt
    16441 ctgttacaga cattccctgc atcagcagct tgagtagcag tcgttctttc tcggatacgc
    16501 taccaggcaa tgacaaatga accgttgtac cgaactcttg cagaaaaaca cgaaaatcac
    16561 atgaattcat gaacacacct ttatctttta tcgctgttca gtatgagtga ttacctgtgg
    16621 attcctgagc acacataagg ggtaaccata aacatcagcg atgccatacg cataacatcc
    16681 cacatcacta tcaaaaagat tatctttacc tctgatgctc gctcaaaaac agttacgcta
    16741 aataccttaa aaaggtaatc acaagtcaaa ctcaggagat aatcacgcaa gaatgcaata
    16801 ttaaaattca gatactcatt tttggtaata aagaagtagt tggactattt caatatttat
    16861 aatttcccac tacgtattga cgcccattca tgagtaaagg cgcattatat gaatttaaca
    16921 atccagatca actgaaaata cctcaacctc ataaacacat agcgtcaaca ttcaatgaca
    16981 taatgagtaa agatgttggt tatgcataca cgtcattact ctatgcctat cccttaacaa
    17041 cctgcttttt aaggctgaat ccattcagca atagatagcc aacattataa caatgacaat
    17101 aaaaacaata tttaaaacaa tcaataaaat atcagacaca gacaattaaa ccttaaacaa
    17161 caaacagtta cattgatttt agttgatttg cgctacgcgt ttcccatgaa taaagcgtca
    17221 tttcaaatta gcaccaatat ttacattaaa taagactaag cttattgaac attcacaaca
    17281 gtccaagcgt aggttttggg cttcaatagt tcttttcttt tttattatct gttgcctggc
    17341 aatcatcaat cgatactaag ggcttaactg tacacagata taacgtatca agaggtaaga
    17401 gaaaccgcgc catatgcaga catggcgcgg ttttatgcaa taacaatcac gcgtccggga
    17461 acatcacgct atggcccggc gcttcacgat ggagatggat aaagttaagg tgccgctcgt
    17521 actgatcgag aatatcggtg atcacctgct ctttgctgta gtccatcagg tcgttgccct
    17581 ggctgccttc taacaggaag gtttccagcc ggtagtaggt cgatttcccg ctacgtgcgc
    17641 ggtaggtaaa gcccggcacc gagtattgct gcggccaaat ctgatagaca aagttttgct
    17701 cttcgcccat atgcaccaac aaatccagat gccccaactg ttgtccctct tccggcggca
    17761 ggttttttag ctccacgtac gcgccgcgca accgcagctc ctgcgccact tcttccattg
    17821 ccgggtaaca gaccgtctcc atcatctgtt tagtgtaacg cgtgcctgga taattcatca
    17881 ggcgcgagag acgttttttc cagctcaggc gatcctgaag ccccagcggt cgcggcgcgg
    17941 tatcacggtt ggcactttca cggcggtaat cttctacctt cagagattta tacaaccccg
    18001 ccatcacgaa gaaaatcaca aagctgaacg gcagacccat aatcaccgtg gtgttttgca
    18061 gcgcggatat cccgttggtc atcagcatgc cgagcgtcag caggccaatc gccaccgacc
    18121 agaagacgcg cagccagccg ggggcgtcgc tgttgatatc tttaagctgc gaggtgaaat
    18181 tccccagcac cagcgccccg gagtccgccg aggtcacata aaacagcagg ccagtaatgg
    18241 tggcgacgga ggcgctaaag gtaaacgccg gatactgcgc cagcaggctg tagaagccgc
    18301 gctccggatg gaccatcgct tcctcggcaa atgccgcgcc gccgtggatg atttcataca
    18361 gcgcgctatt gccgaacacc gagagccata acagcgtgaa ggtaaacgga ataatcaacg
    18421 tgcccagcac gaactggcga atggtacgcc cacgcgagat acgcgccagg aacaagccga
    18481 caaacggcga ccatgccacc caccatgccc agaagaagag cgtccagtta ttcatccact
    18541 caaccggacg gtcgaaggca aaactgttga gcgtcatgcc cataaagcga ttcacatagt
    18601 cgccaacgtt cagcaccagc gcattaagca ggaacgaggt gtcgcccata aacagcacga
    18661 acaggatcaa ccccagcgcc agcgcaacgt tgagttccga taacacgcga atgcccttat
    18721 cgacaccgga ggtgacagag attgttgcga tgatcaccga caaggcgatc agtgccgctt
    18781 tcgccgccat cgaatcagga atatcaaaca gtacgctcaa gccatagtta agctgcacca
    18841 caccgatacc gagcgtagtg gcaataccga agatggtgcc gattaccgct gcaatatcca
    18901 ctgaatgacc aatcggcccg ttaatccgtt taccgaagat cgggtacagc gccgagcgga
    18961 tggtgagcgg caaattataa cgatagctaa agtatccgag cgccatgccc atcagcgcat
    19021 acatcgacca gccggttaag ccgtagtgaa acagcgtcca gaccatcgcc tgacgcgcag
    19081 cctcaatcgt ctgacccgcg ccttccggcg gctgcatata ctgcgttacc ggttcggcta
    19141 cggagaagaa catcaggtcg ataccgatcc cggcagcaaa cagcatcgcc gcccaactca
    19201 gcagactgaa ttccggtttg gattgttctg gcccgagctt caccgaacca aaacgcgaac
    19261 aagcgataca gaccacaaag acaatataga gcgttgccgc cagcagatag taccaaccga
    19321 aggttttaga aacccagtcc agcgtgcggc caatccacag ggccgagaag tcgcgaaaca
    19381 ggatcgttgt cagggaaaac aacaaaatca gtccggcgga ggtgtaaaac accaccggat
    19441 tgattttgtc cttttccctg ctgtgtgaaa ggtctgtcat ccagtatccc cactgttatt
    19501 gttaccattt aaaatcaaat tcgtaacaat taagacacat tttatattga acgtccaatc
    19561 aataaccgct ttaatagata aacaacgctg atgaatggag tggcgaaaat gcccaaattg
    19621 gggatgcagt cgatccggcg cagacaactg atcgacgcca cgctggaagc aataaatgaa
    19681 gtgggcatgc acgatgcaac gatcgcgcag atcgcccgcc gtgcaggcgt ttctacgggg
    19741 atcatcagcc actatttcag ggacaaaaat ggtctgctgg aagcaaccat gcgcgatatc
    19801 accagtcagc tgcgtgacgc ggttttgaat cgattacatg cacttccgca gggcagtgca
    19861 gagcagcgat tacaggcgat tgttgacggg aatttcgatg aaacgcaggt cagtaatgct
    19921 gcgatgaaag cctggttggc gttttgggcc agcagtatgc atcagccaat gctttaccgt
    19981 ttgcagcagg tgagcagccg ccgcttgttg tcgaatctgg tgagcgaatt tcaccgcgag
    20041 ttaccgcgcc aacaggcaca ggaagcgggc tacggccttg ccgcgctgat tgacggatta
    20101 tggctgcgcg cggcactgag cggcaaaccg ctggataaac ctctcgcaca ctcgctgacc
    20161 cgtcacttta ttactcagca tttacccacc gattaaccga ggaaacgtga tgtcccgaat
    20221 ggcagaacag cagctttata tacatggtgg ttatacctcc gccaccagcg gtcgcacctt
    20281 cgagaccatt aacccggcca acggtaacgt gctggcgacc gtgcaggccg ccgggcgtga
    20341 ggatgtcgat cgcgccgtga agagtgccca acaggggcaa aaaatctggg cggcgatgac
    20401 cgctatggag cgctcgcgta ttctgcgtcg ggccgttgat attctgcgtg aacgcaatga
    20461 cgaactcgca aaactggaaa ccctcgacac cggaaaagcg tattcggaaa cctcaaccgt
    20521 cgatatcgtt accggcgcgg acgtgctcga gtactacgcc gggctgatcc cgtcgctgga
    20581 aggcagccag atcccattgc gtgagacgtc atttgtttat acccgccgcg aaccgctggg
    20641 cgtagtggca gggattggcg catggaacta cccgatccag attgccttgt ggaaatccgc
    20701 accggcgctg gcggcaggca acgcgatgat tttcaaaccg agcgaagtca ccccgcttac
    20761 cgcgttaaag ctggccgaaa tttacagcga agcgggcctg ccggacggcg tatttaacgt
    20821 gctgccgggc gtaggtgcgg aaaccgggca gtatctgacc gagcatccgg gcattgccaa
    20881 agtgtcattt accggcggtg tcgccagcgg caaaaaagtg atggctaact cggcggcttc
    20941 ctccctgaaa gaagtgacca tggagctggg cggtaaatca ccgctgatcg ttttcgacga
    21001 tgcggatctc gatctcgccg ctgatatcgc catgatggcg aacttcttca gctccggtca
    21061 ggtgtgtacc aacggcaccc gcgtgtttat tccggcgaaa tgcaaagccg catttgaaca
    21121 aaagattctg gcgcgcgttg agcgcattcg cgcgggcgac gttttcgatc cgcaaactaa
    21181 cttcggcccg ctggtcagct tcccgcatcg cgataacgtg ctgcgttaca tcgccaaagg
    21241 caaagaggaa ggcgcgcgcg tactgtgcgg cggcgatgta ttgaaaggcg atggcttcga
    21301 taacggcgca tgggttgcac cgaccgtgtt caccgattgc cgcgacgaaa tgaccatcgt
    21361 gcgcgaagag atcttcggtc cggtgatgtc gcttctcact tatgaatctg aggacgaagt
    21421 gattcgccgc gccaatgata ccgactacgg cctggcggcg ggcatcgtga cggcggacct
    21481 gaaccgcgcg catcgcgtca ttcatcagct ggaagcgggt atttgctgga tcaacacctg
    21541 gggcgaatcc ccggcagaga tgcccgttgg cggctacaaa cactccggca ttggtcgcga
    21601 gaacggcgtg atgacgctcc agagttacac ccaggtgaag tccatccagg ttgagatggc
    21661 taaattccag tccatattct aaccaggagg tttatttgca atttgactac atcattattg
    21721 gtgccggctc agccggcaac gttctcgcta cccgtctgac tgaagatccg aatacctccg
    21781 tgctgctgct tgaagcgggc ggcccggact atcgctttga cttccgcacc cagatgcccg
    21841 cagcgctggc gttcccgcta cagggtaaac gctacaactg ggcctatgaa acggaacctg
    21901 aaccgtttat gaataaccgc cgcatggagt gcggacgcgg taaaggtctg ggtggatcgt
    21961 cgctgatcaa cggcatgtgc tacatccgtg gcaacgcgct ggatctcgac aactgggcgc
    22021 aagaacctgg cctggagaac tggagttatc tcgactgcct gccctactac cgcaaggccg
    22081 agactcgcga cgtgggcgag aacgactacc acggcggtga tggcccggtg agcgtcacca
    22141 cctccaaacc cggcgtcaat ccgctgtttg aagcgatgat tgaagcgggc gtgcaggcgg
    22201 gctacccgcg cacggacgat ctcaacggct atcagcagga aggttttggc ccgatggatc
    22261 gcaccgtcac gccgcatggc cgtcgcgcca gcaccgcgcg tggctatctc gatcaggcca
    22321 aatcgcgccc taacctgacc attcgtactc acgccatgac cgatcacatc atttttgacg
    22381 gcaaacgcgc ggtgggcgtc gagtggctgg aaggcgacag caccattccg acccgcgcgg
    22441 cggcgaataa agaggtgctg ttatgtgcag gcgcgattgc ctcaccgcag atcctgcaac
    22501 gttccggcgt gggtagcgct gaactgctgg cggagtttga tattccgctg gtgcatgatt
    22561 tacccggcgt cggcgaaaat cttcaggatc acctggagat gtatctgcaa tatgagtgca
    22621 aagaaccggt ttccctctac cctgccctgc agtggtggaa tcagccgaaa atcggtgcgg
    22681 agtggctgtt tggcggcacc ggcgttggtg ccagcaacca ctttgaagca ggcggattta
    22741 ttcgcagccg tgaggaattt gcgtggccga atattcagta ccatttcctg ccagtggcga
    22801 ttaactataa cggctcgaac gcagtgaaag agcacggctt ccagtgccac gtcggatcga
    22861 tgcgctcgcc aagccgtggg catgtgcgga ttaaatcccg cgacccgcac cagcatccag
    22921 cgattctgtt taactacatg tcgcacgagc aggactggca ggagttccgc gacgcaattc
    22981 gcatcacccg cgagatcatg catcaaccgg cgctggatca gtatcgtggc cgcgaaatca
    23041 gccccggtgt cgaatgccag acggatgagc agctcgatga gttcgtgcgt aaccacgccg
    23101 aaaccgcctt ccacccgtgc ggtacctgca aaatgggtta tgacgagatg gcagtggttg
    23161 acggcgaagg ccgcgtacac gggctggaag gcctgcgcgt ggtggatgcg tcaattatgc
    23221 cgcaaattat caccggcaat ttgaacgcca cgacgattat gattggcgag aaaatagcgg
    23281 atatgattcg cgggcaagaa gcgttgccga ggagcacggc aagatatttt gtggcaaatg
    23341 ggatgccggt gagagtgaaa aaatgagtcg ttatgtgaac tgactcagga accgattaga
    23401 ttgagagtta ccgttctact atgaggaccg aatccttata taaaacactg aggtaactct
    23461 catgcttcat atccagtatt catgatgctg gttttttgct gatatttaac ccgaacactc
    23521 ctgatacaga ctattaattc atttatctgc ggcagatttt attttctgga tatattatga
    23581 gtgaacaaat caaacaggat atcgatctga ttgagatttt attttatctg aagaaaaaga
    23641 ttcgtattat cctttttatt ataactatct gtatggcaat ggtgctgttg tttctgtata
    23701 tcaataaaga taatataaaa gtgagttaca gcctaaaaat aaaccagaca acaccaggca
    23761 tacttgttag ctgtgatagc aataataact ttgcctgtca gaccacaatg actgaagatg
    23821 ttattcagcg aattactaca ttttttcaga ccagcccaga tgtcaagaac agagaaataa
    23881 agctggaatg gtcaggagat aagagagatt taccaactgc tgaagcggaa atatctcgcg
    23941 tgcaggcgtc tattatcaaa tggtatgcgt cagaatacca taatggcagg caagttctcg
    24001 atgagataca aacaccttca gcaattaaca gtgagcttta tacaaaaatg atatacctga
    24061 ccaggaactg gtcattgtat ccgaacggtg atggctgtgt aactataagc tcaccggaaa
    24121 taaaaagtaa atatcctgct gccatttgcc tgattctggg attttttcta agcattgtga
    24181 tttctgtaat gttttgcctt gtcaaaaaaa tggtagatga ataccaacaa aactctgggc
    24241 agtaataaac agcattctga tagagagtgc ctgtataaag ctgaagtgac aatgaccggg
    24301 aagggaaccc gttttcagga atggatgcct gtctttttca ctgtaaatat cctggcatta
    24361 ctaatgccag gatatttaca gaagagcagc ataaccatga cggaacgggg ttaagtcagg
    24421 ttgtccttaa cgctattcac aacctcgtat gctgccttca ggtttatgtc cagacttcat
    24481 atctctctca gcaatcctca ataatcagat acaccgcttt caccgggcca tgaaccccga
    24541 cgactttgat aagctcaata tccgccgttg aactagggcc gctaatgatg ttaatgcagg
    24601 acggcattcg ttcaccggcc tgcgctttct gatgcaattt ttctgcgagt tgcgctacac
    24661 gcggcaggat tgtgctttta cgcaggataa aaagagaaga ttccgggagc aggctcaatg
    24721 aacgcccgcg ctcggcggcg gaaaaaagaa ccacgcctcc cgattcggtt aaaccatatt
    24781 cagcatacac gacacccact ttagcctgct ctgcctgtga gatattctcg gcacctttcg
    24841 ccggatccca aacaacgaca ttgcattcct gctgcaaacg ttcgctaatc cccaattcct
    24901 ccagcctcgt gtcaccgcta atcacgaccg actgatctcc cagctcttta cacagacgta
    24961 ttgcagcttc tgccgccttc gcctcgctgg tcagttcaca gcgcgtcaac ataacatcgc
    25021 tggcaaactg aataaacgcg tcacaacgct gctgttgggt aagttgggta agccgctcgt
    25081 tagcatagtt gttaagcggc gcatcttctg cttgcggttc aagtcgcagc gggcgaccca
    25141 gtgcctgagc aacgttattc aaaaattcgc tccgattatc cattcttttt ctcctgcgcc
    25201 tgatgtttct taaaccaact acggaaactc tctccgtcag cttcaggaag atcgcgtgct
    25261 tccatccagt cgctaatcgc gccaaatttg agtggtgttt tgccgccatt gataaaccag
    25321 cttgccgcat gagcaccagc catcatcccg actttccaca atcctggatg actattggca
    25381 taagcgaaca tttttatcgc ccgttgctct gcttttgcgg tgatcccttt ttcagccatc
    25441 acccgacgat gacgcaaaat cagttttgac agcggaatac gcaccggaca cacgctgtca
    25501 caagcggtgc ataaagagca ggcgtagggt aaatctttaa aatctttata gccgccaagt
    25561 agcggagaaa tcaccgcacc aattggccct ggataaatag agccatatcc atgaccgcca
    25621 atatggcgat atgccggaca agtattcata caagccccgc agcgaataca gcgcagcaca
    25681 tcccgaaatt cagaggccag cacctcagaa cgcccgttat cgacaataac cagatgaaac
    25741 tcttcaggac catcaacgtg ccccgcttcg cgcggtcctg tcagccaggt gttgtatccc
    25801 gtcaaacgtg caccaacggc actgcgcgcc agcatggtga tcaatacatc tacctcggca
    25861 aacgtggggg caatacgctc cattcccatc actgcaatat gcgttttagg cagcgtggta
    25921 cacattcgcg cattaccttc attggtcacc aggcataccg aaccggtctc tgccaccgcg
    25981 aaattacagc cggtaatacc tatttcagca ctgaggaaat cttcgcggat tttttgccgg
    26041 atgaataagg tcattgcttc aggcgtttcc gacccctcat agcccagacg ttcgtgtagc
    26101 acccgacgga tctgatggcg atctttatga attgccggga ccacaacatg agagggcgga
    26161 tcttgatcca gctggagaat gtattcaccc agatcggttt caatcacctg aatgccagca
    26221 tcctgcaaca catgattgac accaatctct tcggtcacca tcgatttaga tttcaccacc
    26281 ttccgggcat ttttgcgttg ggcaacctgt aaaatgtagc gagtggcgtc ttctttagtt
    26341 tttgcaaaat agacgtgacc gccgttttgc gtcacttttt ctgagagctg gtacagataa
    26401 gcgtcgagat tactcagaac atgatcacgt atctgggcgg cccgatcgcg ccactcctcc
    26461 cagtgcccca attcatcgac cattttttgc cgatttgccc cgatacgctg ctgcgcgttt
    26521 gccaccgctt tgcgcatgat cggatcttca atttgctgac ggatgcgtgt cttaaaatct
    26581 gtattactgg ttttgatcga catctttata tcctcagcgg ctcatcagca cttcagcaat
    26641 atgcattact ttgactttct gcccttcccg ttgtaatcgc ccactgatgt tcagcaggca
    26701 actcacgtca gcaccaatta aatactcagg gcggacttcc atcaggtgcg caaccttttc
    26761 tttcaccatc tcgccggata tttcggccat tttgaccgag aacgtgccgc caaatccgca
    26821 gcaggtatcc tgttcagcaa aggtcaacag ctccagtcca cgcacatttt tcagcagcat
    26881 aagtggctcg tccttcaccc ccagcttacg ggccaggcta caagatgggt gatacaccgc
    26941 tctcccttgc aaactggcac ctacatcgac tacccctaat gtattaacaa taaaagaggt
    27001 gagatcctgc atacgcgcgg caaccttttc ggcacgtgat gcccattcag gctcatccgc
    27061 cagatacgtt gggtaacttt ttacggcata ggtacaggag ccagccggtg aaataatggg
    27121 atcgtcgtta tcctccagtg cggcgatcag atttttcatc cctggaatcg cttctttgat
    27181 ataaccacta ttgatcgcag gctgaccgca gcatccctgt ttctccggga aatttacgcg
    27241 acagccgagt tttccagtag cagcacggag tctcgtgcca ttcttgattt cagggcgtca
    27301 ccaatacagg tgacaaagaa attgacattc acaactaata ctccattact tcatgcccat
    27361 ttatgcccga tggttccccc cttccggggg atcaatcatc aataaatatg gcgttctttt
    27421 ttaattggct agcaacatac cacccctcat ttgtatgaca aatgataatc tttcatccaa
    27481 acttcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt atagggaaat
    27541 cgacgatcat ttttggcata agaaatccat ttaaaaaggc tacatccctt atacctattc
    27601 agtgctactt attcaggggt tattgagata tacactgatg gtgtacaaac aaaatcacca
    27661 acaataatta cgtatcaagt aataatcggc attttattta attgaagcaa tttagtttgc
    27721 atttttattt aaaaactgag ctagagtcca tcagaaaaaa atatataaag cccaaaccac
    27781 cgttggaaat agaaatagtt gaaaggctca caactcccgg aatgcggatt atctttgacc
    27841 tgatttctgg cggttttatt cagggtgcaa gctgtctgac cctttcccga tattctcccg
    27901 gagtacaacc aaactcgcgg acaaacgcct tgtgaaaaga tgattcactg gcatagccta
    27961 ctgactcagc gatcaccaca acagggagca tttcccggga aaacatctgg gccgctattt
    28021 gtagacgcaa ctttgttaat acagctagcg gcgtggttcc ggaaacatca cggaaaagct
    28081 gggcaaaact tgcccgggac atgtgggcga tgctggccag cgattcgacg gtccaggcgt
    28141 gtcctggcat ttccagcatt tgctgtatta ccgcaccaag acgtggatgc agaagcaaac
    28201 tgagaatgtt tttctctgta ttaacctgtg caatccactc acgcaccgcg agggtaaaga
    28261 atgtagcgca gatctggcta cacagagcat ccactcccgg cattgccgat ctggattcct
    28321 gttgtaaaaa cgggatcgcc tccctcagcc agttatattc cacgctatgg ttcactggtg
    28381 ctacaaacag cgtttccggc aaagacgtta aaaaataacg cgccgaatgt tgcagccgaa
    28441 gagtgccaca gacaatacag gtcgattcat tatcgacatg actcaggcga tgagctgaat
    28501 tttgtggtag caggaccaca tttcccgggc gtaatgtaaa aatctctccc gtcggcattt
    28561 ccagcttcgc cgctccttgc gttaacgcat gccaacgaat aaccgacaat tccccggcac
    28621 catgtggaag ttgccagtca cttcctaaca cgcaattctt atcgatcgtt ccttgtggag
    28681 cgttaagcat caacaaacgg ctaagagcat ccatatcaga ctcctgagta gttttttgat
    28741 tacaaacacc aactatctta gtcaataatt aatctaatac catcaaatag ttaatcatga
    28801 tttttgtttt gtgattctga ttaagaaaaa gaaaatacca gacgataaga caaaatatca
    28861 agcgttgtga agaaatgtta tttgctcttt tgcgtctata gtcatgatgt caaatgaacg
    28921 cgtttcgaca ggaaatcatc atgaataaat atcaggcagt tattattggt tttggcaagg
    28981 ctggaaaaac attagccgtc acgctggcaa aagcaggttg gcgtgtggct ctcatcgaac
    29041 aatcaaatgc aatgtatggc gggacctgta ttaatatcgg ctgtatccca accaaaacat
    29101 tggttcatga cgcacagcag cacacagatt ttgtccgtgc catacagcgt aaaaatgaag
    29161 tggttaattt tttacgtaat aagaattttc ataatcttgc ggatatgccc aatatcgacg
    29221 tgatcgacgg ccaggcggag tttatcaata atcatagcct gcgtgttcat cggcctgagg
    29281 gaaatctgga aattcatggc gagaaaattt ttattaatac cggtgcacaa accgtggttc
    29341 cgccaattcc tggaattacc accacgccag gagtatatga cagcaccgga ttacttaatc
    29401 taaaagaatt gcctgggcat ttaggtattt taggcggcgg atatattggc gttgagttcg
    29461 cctctatgtt cgctaatttt ggcagcaagg taaccatttt agaagcagct tcgctgtttt
    29521 tgcctcggga agatcgggat attgctgata atatcgcgac gattttacgc gatcagggcg
    29581 tcgatattat cctcaatgcc catgtggagc gaatcagtca ccatgaaaat caagtgcaag
    29641 tgcatagcga gcacgcccaa ctggcggtgg atgcactgtt aatagcttcc ggtcgtcaac
    29701 cggctaccgc ttcgttacat ccagaaaatg ccggtatcgc agtaaacgag cgcggggcaa
    29761 ttgtcgttga caagcgatta cataccaccg cagacaatat ttgggcgatg ggagatgtta
    29821 ccggcgggct gcaatttact tatatttcac tggatgatta ccgcattgta cgtgatgagt
    29881 tactgggtga aggcaaacgt agtactgatg atcggaaaaa tgtgccttat tccgtattta
    29941 tgacaccgcc cctgtccagg gttggtatga cagaagaaca agccagagag agtggtgctg
    30001 atattcaggt ggtgacattg cctgtagctg caattccgcg cgccagagtg atgaacgata
    30061 cccgtggggt attaaaagcg attgttgata ataaaaccca acgtatatta ggggcatcac
    30121 tgctgtgtgt tgactcccac gagatgatta atatagtgaa aatggtgatg gatgccgggc
    30181 tgccttatag catattacgc gatcagatat ttactcatcc gtcgatgagc gaatcactca
    30241 atgatctatt ttcattagtc aaataaactc aaaatcagac gccagaacaa atattctggc
    30301 gtctcagaga aaagaatctt attaattctc tgttactcta taacttctta atcacttcat
    30361 tgatggtatt ttatatgttt aaaaaatctg ttttatttgc aacactatta tctggcgtta
    30421 tggcattttc caccaatgca gatgataaaa taattctgaa acatatcagc gtctcgtcag
    30481 tatcagcatc accgacagtt cttgaggatg ccattgctga tatagccaga aaatataatg
    30541 cttcatcctg gaaagtcaca tcgatgcgaa ttgataataa ttcaaccgca acagcagtat
    30601 tgtataaata aggatgttca caatggaaaa atacctgcac ctgttaagtc ggggagataa
    30661 aattggcctg acattgattc gtctgagtat tgcgattgtt tttatgtgga ttgggttatt
    30721 aaagtttgtc ccttacgagg cagacagcat tacaccattc gtcgcaaaca gtccactaat
    30781 gtcgttcttt tatgaacacc cggaagacta taaacagtat ctgactcacg aaggcgaata
    30841 caaaccagaa gcaagggcat ggcaaacggc caataatacc tatggttttt ccaacggtct
    30901 tggcgtcgtg gaggtgatta ttgctctgct ggttttggct aatcctgtca atcgctggtt
    30961 aggtttattg ggagggctga tggcatttac cacaccgttg gtaacactct catttttaat
    31021 caccaccccg gaggcatggg tacccgcatt gggtgacgct catcatggtt tcccttattt
    31081 atccggtgct ggtcgcctgg tattgaaaga tactctgatg ctggcaggtg ctgtcatgat
    31141 aatggcagat tcggcgcggg acattcttaa acaacgcagt aatgaatcca gttcaacgtt
    31201 aaaaattgaa tattgataat ccgaacactg tctgttgttc accttttcag gcgtcaggcc
    31261 ccctttctga tgatgaagcc tgacgccata ttaccggaga tatgacagag tgaagatcaa
    31321 tatcactccc ctgttaaaag attcttgtcg tctcaggtaa aatacatgat taatatcaat
    31381 catgtatttt tacagcaaga caaccacgaa caaactcagg attaaaatgt tttaccgatt
    31441 ccccaacata acccaaatca agcgaactaa tttgcgccat ttctttctct gtcagtgaga
    31501 aatcccaaat agcaaaattt tcttcgatac gctgttgatg tgtcgatttc ggaataacga
    31561 ccactccccg ttgaatattc caacgcagaa tgacttgaga aatcgatttt tgatgggcat
    31621 ctgcaatact ctgaagcaga ttattttcaa agggtttatg tcgtccacca cctaacggag
    31681 cccatgcttc aggctgcacg ttataatact tcatggttgc cagcgcctct ggttgggcaa
    31741 aatagggatg caactcgacc tggttgacca tcggtttaac tcttaccgtt tcacaaaagt
    31801 tcgccagaac atgaggatag aaattggaaa ccccaatcgc ttttaatttg ccctcttcat
    31861 aggcatcttc gagtgcacgc cacgcactga aataatcgcg catagcctgg tgcaataaat
    31921 agaggtcgaa gtattctagt cccgattttt ttaatgacgc ttcaatacct gcttttgccg
    31981 tatcctgatt caacatatcc tgcacccaca atttagacgt tataaataac tcttctcggg
    32041 tacataaacc ttcagatatg gcttcacgaa cggcttcacc gacggcatct tcattaccgt
    32101 ataccgctgc agtgtcgatg agtcgataac ccgtgcggat agcattcagc actgactgtt
    32161 tgcacacgtc tttatcggta acctgaaaaa caccaaatcc catcatcggc attttcagat
    32221 tgttacttaa tacggaaaat tccactgtca gtccctccat taggcatgaa caatgagtct
    32281 acgttaaaac gtaacctcaa attagtatgt ggattttgat atcacttatg caaaaaattc
    32341 attaataatg taggactgaa acctctctat tttcggggac aacgaagcag acgctaccag
    32401 tgcttttgcc ttcgcccttg ctgtttttga tacacttagg gcccagggca taacgaaaat
    32461 gtgcgatatg acaaagaatt aacggagaat gtgatgatca ggcagaagat tctacagcag
    32521 ctcctggagt ggattgagtg caatcttgag caccctattt caatcgaaga tatcgcacag
    32581 aaatctggct acagcagacg caacatccag cttctgttcc gaaatttcat gcatgtgcct
    32641 ttgggagaat acattcgcaa acgaaggctt tgtcgcgcag ccattcttgt ccggctcacc
    32701 gcgaaatcta tgcttgatat tgcactctct ttgcattttg attcacagca gtcattcagc
    32761 cgcgaattta aaaagttatt cggctgctct ccccgtgtat accgccaccg tgattattgg
    32821 gatctcgcaa atatcttccc ttctttttta atacgtcaac agcaaaagat ggagtgtaga
    32881 ttagtcaact ttcctgagac acctattttt ggcaattcat ttaaatatga tattgaagtg
    32941 tcgaataaat caccggatga agaagtcaaa ctgcgacgtc atcatttagc cagatgtatg
    33001 aagaatttta agacggatat ctatttcgtc tccacgttcg aaccgtcaac aaaatcggtc
    33061 gatttgctca cagttgaaac ttttgccggt acggtatgca aacaaaccga ttcagagatg
    33121 tcgaaagagt ggacaattaa ccgaggactg tatgcctctt tccgctatga aggtgagtgg
    33181 gagcactacc ctgaatgggc gcgaaacctc tatctgatgg aattacccgc cagggggtta
    33241 gccagagtga acggcagtga tattgagcgg ttttattaca atgaaaattt cgccgaaatt
    33301 gatagcaata acattgtttg cgaaattttt atccccgttc gtccggttta gcctgtaaat
    33361 ccatcacata agatttaccc taccctgcca ccgtctaatc ggtaccaggg taggtattca
    33421 gtaaataatg gcgctgcgca tgtcaacgcc attattattt attacatcgt cgtttttatt
    33481 tctcctcagc accttcagta tctgcaggaa cggcgttaat tgtcacagtc actggtttca
    33541 tttcataccc tccatatgtc aaagagttga atgtgaatgt gtatgtacct ggcttgttgg
    33601 tactaaatgt acgcgtgatt ttcccgctcg cgaaatggtc atttttcgac ggcaggaact
    33661 gataatccgc ctctgtcacc ccttccggag cctcaatgtc gacccacatg gttcctccta
    33721 cggggttgac acccgacatc agagtaaccg tcagtgttgc agtagtcttc ccgtccgcca
    33781 ccagagaatc tacacgctct ggagatgcag cgagctccgc gtttttatca tccgcgacga
    33841 atttagattc ggcagttttc gtcgattcct gaccgtcggc ctgttccact ttcgccgtga
    33901 gtgtatatgt cgctgcgaca gtggtcgtgg cggtgaaaat agcctgtcct tgctcattcg
    33961 ttgtcgccgt cccatcggga tccagagcta aatttgccgg gctagcagtc agcgtcacct
    34021 cgctatcttt caacaggttg ttattggcat cagtcaccgt aactttatac gtctgtttgg
    34081 ctttgccatc cgccagtgca tagttgccaa tcacctcaat accggagacg gttgcggtcg
    34141 ccttatcggc cacaaacgtt atagtctgcg catccgaagg ctggtcattt cctgccgatg
    34201 ccgtgatctc ataggttccg gcagtcacgg aaaccacctg caactctgct ttcccctcat
    34261 cgttggcttt cacccagaca ttatcgccag taagcggctt gacaccccgg ggcagattaa
    34321 aggtcaccag agtgcctgga acagggttac cgtttttatc ctcaacggtc gccgtcagcg
    34381 taaaggcatc tttgccgttt gccacttttt gaacggcggt gtcaacctgc aggtttgcct
    34441 gaccggtgct ggcatccgcg ttgaatttca ccgtgaccgt cttctgagag tttttcaccg
    34501 cggccgcgat ttcaagttcc cctgcaacgg ttgatatcaa ctcaatatcc gctttacctg
    34561 ccgcattggt tgttactgta ttccccgtct tgctagtcac accctgcggt aaagtcagcg
    34621 taacttcttg cccctgcaac gggttaccat agctgtccac gacggtcagg gttatctggt
    34681 tagcagactg tccattagcc agttggttat taaccttcac tgtcatatca cgaatctcag
    34741 cctgagaagc gtcccctgca acgttcagca ccagtggctg agcaacagca ttttggccgt
    34801 tcactcgcgg catcacagac aactgacccg cggcagatcc cagcgttaag gtcgacacgt
    34861 agaccccatt acctttctct gtccagtttc ctgctgaagg acgctcactc cccgtgctgg
    34921 cggcaccgct aaacactggt gcatctggct tcagcccggt gaccggattc ccgtacgcat
    34981 ccttcacggt gaaggtgagt tccgtcgtcg ttttaaccgt tggagtttta ttgtccgcaa
    35041 caagcgtaga attcgctgat gacatctctc cggcaataac agtcagctgc gcggcttcgg
    35101 tggctgctgg ctggccgttg aagagcggca tgacacgaag ctcgcccgtc tttccgcctg
    35161 tagttaacgt agcaacatag gaaccgtcac ctttttcggt ccaactggaa acggtcgccc
    35221 cttcagaggc ggtccccgtc aaacttgccg acaacgaaag accactgata gcgttgccat
    35281 gtgcatcttt cgccaccaac gtcacggttg tgctttcgcc ggcttttacg tgatcttccg
    35341 cgacagagac tttcgactgg tttgaagata acgcatcagc caccacggtt acttttgccg
    35401 catttgctgc cgcatcctgt ccatttagct tcggcataac ttctaattca cccgccgtag
    35461 agccgagagt aatctgcgca gtccacgtcc catcaccatt atttgtccag ccagatgccg
    35521 tagaaccaac agcagcggca cccgctaatg acggcgcttc cggcgtgagg ctggtcacag
    35581 ggttgtcatt ggcatctttt gtcgaccaga ttgccgtaag cgtaccgccg accaccggtt
    35641 tatcaggatt cagggtgatg gacgaatgtg ctgcatcaag cggcccggca acaaacttca
    35701 gcgtttgttg taatgtgccc agctgatact gctcgctgaa ggcgcgcaca acaacgtttc
    35761 cggcacgggt cgaagaaacc gtggcgctgt aaacccctgg ttttatttcc gaaatggcac
    35821 caacggtaac accattagtg tcttgcggaa caagtcgcag gcggctggct tctcccgtca
    35881 ccggattacc ctcggagtcc accgcagtca gcgtcagcgt gtaggcttgc tgaccatcag
    35941 caaccacgtc acctgacggc tcgttagcgc tcagggtgga gtttgccaca tccatcatcg
    36001 tggcccgcag ttctgcagtg acggttttgc tcatgccatc aacgctaaca gtaatcgttg
    36061 cctcacctga ctgcgttccg gtcgtaaaga cagactggta cacccctgct tcagtttcgg
    36121 tgaactcacc cagtgtgggc tgtgcctgtg atttagtggc cttcagggta cgagtcacaa
    36181 tatttccagc cggtttgaag gttagttcag tcttgatctg ctccttcatg cccgtgactg
    36241 gctgaccctc ggcgtcgcgc agagacagca ccagcggctt ttgctcatta ccgttagcaa
    36301 gcatttgaat acggctctga ccgtcaagcg ttaacgccgt gcgatcggcg ctcataccag
    36361 ctccggtaat aaccacttct gtctgcacac gtttcgaggc attgcctttg ttatcgtagg
    36421 caactgctga aatcgcataa taattgtctt tgcctggacg ataagccggg agcgttactt
    36481 gccactgact accctgaccg gtaattttgc caccttcagc cagtaatgac ggcgcttccc
    36541 actgcacatt tttcagtccg tgtgttgctt tgctgaccac aagccccagg gaaaccgtct
    36601 gaccgccctt accttcaata cgctcaggca gagcaatacg gatcacttca gatttgcggt
    36661 actcaagaac gatgttgtta ttacgctcaa ccaggtcata gcggctgcct gccagcattc
    36721 gacgctcgcg aatgctgtct gtatcgagtt gtttttgcag aggttcgcca atccgataat
    36781 taacttccaa gccaaagcga gtgtcattct caccactctt gccctgctta tgcccggcac
    36841 tcagggtcag aagaggcact ggcgtgtaat tcacttcagc ggtaatcgca tgtggatctt
    36901 tctggcgttt atctttgcca aacagcccga cttcatcgcc ataatactgc tcatacatca
    36961 ggcttgcgcc aagctgcggc caggcgggta aatagccctc agcacgaata tcccagccat
    37021 tcgccgggcg ttcctgataa tcctcaacat ccggcgattt tttccagcca gaagcccgga
    37081 tataaccatt ggcgctcaat ttcaaataat cacgccagta ttccgcacca acaccaatgc
    37141 gggtatgact acgggataaa tcatggtcga taaaggtgtt cacccccgcc atccagtcat
    37201 ttcctgaaaa atgacgccag ccaaaaccaa tatttgactg agtacgatcg tcggtacgat
    37261 gtattgctcc ctgagtgaac aacatatttg tcggcgtatc ataaatcgga taaagcattt
    37321 ccagcgaaga atccttcagc gagaattctt tatcgacatt cagtttgacg cgcgcagtac
    37381 catatttccc gagccactcc tgtatttcct ggttagcttt agctgtggcc attccggtaa
    37441 taaagttacg tgtcgcatcg ctatctggct gactgcttaa aaatgtcccg gcatttgcgg
    37501 caaacgacgc gacatttttc tccacattat tatcagcagc tactgtggta ttttccatgc
    37561 tcaaacgtgg ctgaaccgta tgctgcgcac gtgctgccat tactggcgta aaggtgacag
    37621 cgagtggaaa aagaacctga acagagatat ttgcccacgc cacgcagcgg gccagaactg
    37681 aataacgaaa tcgtggttgt ttatgacctg ttttataacg tgacattgag ataatttcct
    37741 gatgaacgaa ttaacgaaca taggcagaca caaataatgg cgtctgcttg tcagaaaaac
    37801 cggggatttc ccggtttcct tttccgcaga aaaggaaata cctttatgta agaaagtaca
    37861 ccgccaccta atatatccag tgtctgtagc cactcatcag gtggttgggc gcgggattat
    37921 atttttattt atgggtaaaa atttagcttt ttgtgcaagt tattaaatag atacttaagt
    37981 tatatttctt actctgcggg cagtgaataa ttcattatta agagtgaata ataatgaccc
    38041 gtctttaatt aactgctatt gggagtacgt cgaagtgagt atatacgcca ggataaacca
    38101 atatattttt tatggacatt catttatatt catcggactg taaattccgg ggatatcgaa
    38161 cttgcacgac taatctcaac cctcaaacag atttatgaat tttgttcata ccccctgaca
    38221 aatcctgccg tctaatcgag cttttccctt tacgttatgc ttctcatgaa cgataacgga
    38281 aggataacac catgcaaaaa gtaaaactga acaacggtat tgaaatgccc ctgttgggct
    38341 ttggtgtatt tcagatgacc gatgccgccg aatgcgaaag agcggtcatt gacgctatta
    38401 acagcggtta tcgactgatt gacaccgccg cctcttatca aaatgaaatc caggtcggaa
    38461 acgcactgaa acaaagcggt atcgcccgta acgaactctt tgtcaccacg aaactctggt
    38521 tgcaggatac cagttacgaa ggggccaaag cccagttcga acgctctctg aatcgactgc
    38581 aacttgatta cgttgacctg tacttgattc accagccata tggcgatgtc cacggagcct
    38641 ggcgtgccat ggaagagctg caacaggccg gtaaaattcg cgctattggc gtcagtaact
    38701 tccatcctga ccgactggct gaccttatcg cctttaataa cgtcgttccg gcggtaaacc
    38761 aggttgaagt taaccctttc aatcagcagc tacaggccgt tccgtggatg caaagtcgtg
    38821 gtattcagcc agaagcctgg gcaccgtttg cggaagggaa aaatggtctg ttccagcatc
    38881 ctgttctcac ggcaattggc gagaagtatg gcaaaagcgt aggccaggtt gttctgcgct
    38941 ggatctacca acgaggcatt gtttcgcttg caaaatcagt acgcagagaa cgcatggaag
    39001 agaacatcaa cattctcgat tttgaactca gtcctgaaga tatgctgcaa attaccgccc
    39061 tggataccgc aaccagcgcg ttcttctctc accgcgatcc cgcaatggtg gaatggctga
    39121 ctggccgcaa acttgatgtt taagccgcga taaaagaggt attcattcaa tgcaaaaacg
    39181 ttatctgggt aaatccggac tcgaagtctc cgcccttggg ctcggttgca tgggcttaag
    39241 ccacggctac ggcccggcga ccgatacccg tcaggctatc gaacttattc gtgctgcggt
    39301 tgaacgtggc gtcaccttct tcgataccgc cgaagtgtat ggtccgtatc tgaatgaaga
    39361 agtggtcggc gaagccttaa aaccgtttcg tgaccgcgtg gtgatcgcca ctaaattcgg
    39421 ttttaccttc ggcgatgata ataagcagca gattttaaac agccgtccgg aacatatccg
    39481 tgaagccgtt gaaggatcat tacgtcgtct gaagacagat gttattgacc tgctgtacca
    39541 acaccgtgtt gatccggatg ttcctattga agatgttgca ggaacggtga aagacctcat
    39601 cgcagaaggt aaggtcaaac atttcggtct gtccgaggct ggcgcacaaa ccattcgtcg
    39661 tgcacatgcg gtacaacctg tcaccgccct gcaaagcgaa tattccatgt ggtggcgtga
    39721 gcctgaacag gaaattctgc cgttgctgga ggaactggga attggttttg tccccttcag
    39781 cccactaggt aaaggtttcc tgacaggggc gattaagccg ggaaccactt tcggcaaaga
    39841 tgattaccgc agcaccgtac cgcgtttcgc agcacaagcc attgaagcca atgaaaaact
    39901 ggtcaccttg ttaggtaaac tggctgcgga gaaaggggta acgtctgcac aaatcgcact
    39961 ggcatggcta ttagcacaaa agccgtggat tgttcccatc cctggcacca ctaaactgaa
    40021 ccggctggaa gaaaacctgg ccgctgctga cattgttctt tcgcagaaag atacgcagca
    40081 gataaccgag gcgctggaaa caattaaaat cgtcggtgaa cgttactcgc ctgaacatca
    40141 ggcacgcgta ggccgctaat acccaaacgg gtccaggata aaggacccgt tattcggtgt
    40201 agcgaagcgc atcgatcaac agcgcaaaag ctggcggatg ctgcttgcgg ctggggtagt
    40261 aaagataata cccaggaaaa gttggacacc actcctgtaa cacctgaata agcttgcctg
    40321 actttatata ctcctgaacc atgtcttcag ggacacaagc gatcccaaac cctgataagg
    40381 cggcatcaat tctttctggc aacggactac acgtcagctg cccatcaacc ttaacgcgaa
    40441 gcggtttccc ttctcgctcg aactcccagt ggtaaagccc accggcagtt ggcaaacgca
    40501 tgttgatgca gcgatgattt tgtagctcgt gaggcgtttc aggcgcaggg tttgcagcaa
    40561 aatacgccgg agctcccacc acagccatcc gcatatctgg tccaatcctg acagcaacca
    40621 tatctttatc aacattttca cccagtcgta tcccggcatc aaaacggcct tcaacaatat
    40681 cgacaaaacc gttatccacc accagttcta cattgatttc gggatactct ctgagaaaag
    40741 gttttagctt cggccagacc agacttcgcg cggcatgttc cccggcagat aaccgaatat
    40801 tcccggaggg cgtgccgttc tgttgaataa gtgactccag ttcctgttca agctcgtcaa
    40861 tacgcggttc aaggcaggca atgattctct cccccgcttc tgtgggagcc acactgcggg
    40921 tagtccgtgt caggaggcgg atattgagcc tttcctccag agccttcatt gcatggctaa
    40981 gtgcagactg agagacgccg agtttgcccg ctgctttggt aaaacttcgc tctcttgcca
    41041 ccacaagaaa gatttgcagt tcgttgaagt tttctttgag cattggcgat ttccttgtag
    41101 aagcatcctc ttcacagaca ctcatgaagg ggggcataga tgctattatt ttaaccacat
    41161 tattagtaac aatgataatc tggctgtatc gaaaataatt ctaaactttc aggagattta
    41221 ttatgaataa acgtatcctc acgattgcgc cattcttctt accgatattc tgacacacat
    41281 cattaacttt ctatttttac tcttttctcc accataaaat caataaaagc acgaacttta
    41341 gagggcattc tcacccgtga tgaaacatat atataaaatc caggaaatga aacggcccaa
    41401 tcctcaagaa tcggctgtaa cttgcccgca ttgatatgtt cagcgattgc aaaatccaga
    41461 tgttgaatga tacccgttcc attcagcgcc attcgtatca tgctttcgtc atcattagaa
    41521 atatacttac ctgaaacctc tatttcgaac tcttcattac cccccacaac attagaaaac
    41581 tcccatgcag agagtgcacc gctactttta tgccgcaacc gaaggcaatt atggttactc
    41641 agttcctgag gcgtttcagg aataccgtgt tcttttaaat aatctggcga tgccacagta
    41701 accagtttaa taagcggaga aacaggaacg gcagtcatat gctcatccag tccttgctca
    41761 agacgtatgc ccacatcaca gccgtccgca ataatgttag ataaaccgtc atccataatg
    41821 agttctatct ttgttttggg gtaacgagta agaaactcgc caatatgagg ctcaacaagc
    41881 atccgtgctg caacacggga ggaatttatt ctcaacaggc ctgtaggttc aagttgggta
    41941 tcaccaacat ctttcaaagc atcatcaatt tgccccaaag ccgacaccag cacttcatac
    42001 agacgctgtc cttcctcagt aagcgacata ttgcgggtcg tacggtatat cagcttggtg
    42061 ttaagacgtt cctcaagaga ttttaaattc tgggacaatg cggcccgcgt aatacccatt
    42121 tcagaagcgg ctctggaaaa actgccggca cgaacaataa gcgcaaacca ggcaagagaa
    42181 ggcaaaattg aaggctctat tttcatcatt gtaaacctta acttaacact gtgatagata
    42241 ttagaccatt gatgataacc aatacaagtc atactattac gccgttctcg atatgaacac
    42301 gaatgccagt tgcggataat agaaatgata tttaaatctg tttaggcaaa tccccggagc
    42361 ttgaaatgaa aactgtttcg ataaaacata cttactggga tatagctgct gatatctatt
    42421 tccctgacga ttttaatagt gaaaaaaagt atcctgccat tatcagcgcc catccaattg
    42481 gaagctgcaa agaacagaca tcgggtagtg tgtatggtgc cgccctggca aaagctggct
    42541 ttatcgtcat tgcttttgat gccagttttc agggaagcag cggtggagaa ccgcgttatc
    42601 tggaagatcc aacaatgcga gtaaaagact tcagcattgt cgttgattac cttaccacct
    42661 taccttacgt tgatgccggg cgtattggtg tactgggtat ttgtggtgga ggtggatatg
    42721 ccattaatgc cgcaatgaca gaacgtcgaa ttaaagctat tggtactgtc acgggtgcaa
    42781 actacgggcg tcttatgcgt gagggattta cagcgtttaa tcctatcgga gcactcgaag
    42841 cgatggcgca acaaagaaca gacgaagcta acggcgcaaa acttcgcgta gatgatttac
    42901 tcccctcttc tccggatgca gctcttgaag cgggattaac agaaatagac ctttttgaag
    42961 ccacagaata ttatcgttcg ccacgaggct gtgcacctaa tggggtaaat cgttcgctat
    43021 tctcacatca gactgttgcc gtgggttggg atgcatttca tcttgcagaa gtcctgttaa
    43081 ctcaaccaat aatggttgtg gtgggcgata gagtgggcgc atttggtgcc taccgtgacg
    43141 gctgcgaaat tattggccgc gctgcctcaa aacataaaga gctggtcgtg gttgaaggtt
    43201 attcacatta cgacctgtat gataagccag aacctgtgaa acaggcactc gaaaaattaa
    43261 ttccttttta taaaacacat ttgtaataat cttacaggga gattccttat ctccctgaca
    43321 tttatccttt attgactaca caacaataat aaaattactc aaaaagaatt ctattaaagg
    43381 aaaaaaagca tcaaaaagta gcaactcaac ttattaagat tatacgcatt aaaatcagat
    43441 aaacatttga ggtattgtta tgacaaataa acacccatca ttgttttctc cttttatgct
    43501 gacagaaaaa ataaagctac gtaatcgtat cgttatggct cctatgacaa cctggtcagc
    43561 taatcctgat ggaaccatct ctgagcaaga actcgaattt tataaacgtc gctcacaaaa
    43621 tgttggttta gtaataacag gatgcaccta tgtcacgcca agcggcattg ggtttactca
    43681 tgaatttgcc gcgtatgacg accgtttcat taacagcctt gaaaagttag cagctgcagc
    43741 caaaagtggc ggtgctccgg ctattttgca gattttccac gctggtaata aagccatccc
    43801 ggagcttgtg ccaaataacg atgttattag tgccagcgca tcaagcgtaa aatctggtga
    43861 ctttatgaag agagtggtac aaagcaggga aatgactgaa aacgaaattc aggaaaccat
    43921 tagagcattc ggagatgtca caaaaagggc gattaaagca ggttttgatg gcgttgaact
    43981 tcatggtgcg cacggattcc ttctgcagaa tttcttctcc cctctattta accagcgtaa
    44041 tgaccgatgg ggaggagatc ttgaaggacg tatgcgcttc ccgctggccg tattacagga
    44101 agtaaaaaat gtagtctatg aatatgcgac aaagcgcttt gctatcggtt acagaatatc
    44161 tcctgaagaa tctgtaacag gggggctgcg gatagaggat acttataaac tccttgatcg
    44221 actcatttca tccggtattt cgtatataca tacctcgctg gtcagtatta acgatagcta
    44281 ccctgttgag tcgccaaatg gcccccgaac tattgaattg attttaaatc atattgcagg
    44341 cagagtgccg gtaatcgctg caggtaaaat acgcacaccg tctcaggctc aagaagcaat
    44401 ttccgctggc ctgccacttg ttgcgatcgg taaagggctg gtgataaatc cagaatgggt
    44461 gactttagct gaatcaggta gaagccatga aattcaaaca acccttaatc cacaacttgt
    44521 tcctgaatta accattccgg ataaactatg ggatcaaatt caggcatcga aaggaactgg
    44581 ctggtttccg ttaatggatt aaatatctat tactctcccg agagtaaaac tattaaacca
    44641 atgagtaacc gatacagcct cgcaaattct ttcagataat taactcgtca ctgcggcacg
    44701 ttctctcgta aggcgtgcct gctgtgtaat ccagtatgta tccggcatag ttgacggttt
    44761 aatagaactc aattcctctc ccaggtcacc aacaacgata tcttgtataa caccccagga
    44821 gatacgacat tatgatgaga tgcgatatgc tcctcagcgc caatcaacgc ccgggctggg
    44881 ccagttttga gatcagactc attcccggac tgacttcagg cgacatatat caatgacgtt
    44941 ggggcaataa aagatagccg atgtaataca tctattcaaa tttgtgatac atgtcaaaaa
    45001 attggttgac caaactcgtt atttatataa gggcacttac gaagtgcact cttttttaaa
    45061 gcgaggaagt accaatgaaa gagaataaag tacaacaaat cagtcataaa ctgattaata
    45121 tcgttgtttt tgtcgcaatt gtagaatacg cctatttatt tctccatttc tattaataac
    45181 ggcaataaac tgttcacttc agtgatatta aaaaatatgc atcctctccc ttttttgtaa
    45241 gtaattatta tatccgtggg agaggaatac acgctgtccg gcaatcaaac tgcgataaat
    45301 catcggccag taaaatggag atagcctcca ttctcgaaaa attcatactc tcagcgaaac
    45361 catcactcat caaggcgttt atgggagcgt cgccaatggc tgctaacaat gccagacttc
    45421 cccgttgccg aaattccaca taccacaaat ggtcacaggg attgggtgtt gaaatgatcc
    45481 ggatgagcat gtatctttat ggttatgtta taacataaca ggtaaaaatg atgaagccca
    45541 atatccatcc tgagtatcgt actgtggtgt tccacgacac cagtgttgat gagtacttta
    45601 aaatcggctc gactatcaaa acagaccgtg agattgagct ggatggcgta acgtatccgt
    45661 acgtgacaat tgatgtctct tctaaatcgc acccgttcta tacagggaag ctgagaacag
    45721 tggcatcaga aggaaatgtt gcacgattca cccaacgttt tggtcgtttt gttagcacga
    45781 aaaagggggc atgatgaaag tccttaactc tctgcgtacc gcaaaagaac gccatccaga
    45841 ctgtcagatt gtgaagcgaa aaggacggct atatgtgatt tgtaaatcta atccacgttt
    45901 taaggccgtt cagggtcgta agaaaaaacg ttgattcaaa attcgacgaa ttaacgatat
    45961 ttgtctgatt aataatcaga ccggattaat gttggtgtat ttattacacc agcattaatt
    46021 ttcctgggga tgtatcttcc tgttcattta aggtcaactg cctgacgttt ctctccgaat
    46081 attccattat attaatgttg acttgttgac caggtgcgcc ctgtatgctg gcatcaactc
    46141 tcttttagac tgaacacgcc actcagtttc ctccctttgc gacgcagcct gcatttacac
    46201 tcaaacggtt aagatgataa atgtggtaaa tctgttggta ctgacataaa aacgtttacg
    46261 ccacaggaac ggcctgatcc accggtaacc ccatcgccga cgttcgagtg ccagttagag
    46321 taacgcgcac agatgactga atgcagtgcc ctggcaaaga ggccatcgtt cctgtgacaa
    46381 ctggcagtct tcgtttaact tcacttaatt tggctcttag ggggcttacc ggacagatga
    46441 cgtacttaca cctgtttaat ttttcatcac ttattgggat gaacacccat aaccattttg
    46501 tgcggcatgt taatccatta aaacacctta ctgattggaa aatcatcttt aattataact
    46561 catgatagtt ttatattcta tttcttatca tttaaacttg tttttttact agtccattac
    46621 acaacacatt aagactattc ctaacacctc agggcaaagt tcctggctaa tataaaatgc
    46681 aagtaagaat tgaacgttat attgccaata accttatgaa actgaatgtc tttttcttct
    46741 tatcaaaaaa gcaatatttt cattttttgt aaatattgac ttaaccatgg aattcctttt
    46801 ctgttcacat attgacactc atcaggaaaa aaacataaat ttaaacctaa tcgaaataat
    46861 taaaacttaa tctcgtttaa cctatattga tatgtgctac gtatcttatt tacttccgat
    46921 ttactaaaga aactgaatgt acctgtaaaa attacaggtt tggaaagtag tgacatggca
    46981 aaatgattac agcagggact atgaggttaa aaaccatatg gaatgtcaaa accgttctga
    47041 taaatacatc tggtctcccc atgacgccta cttctataaa ggactatctg aactgattgt
    47101 ggatatcgac agattaattt atctatcgct ggagaaaatc agaaaagatt tcgtgtttat
    47161 caatctcaat acggattctt taactgagtt tataaaccgt gataatgagt ggttatccgc
    47221 ggtaaagggg aaacaggtcg tattgattgc ggccagaaag tcagaagcct tagcaaatta
    47281 ttggtattac aacagcaata ttaggggcgt ggtatacgct ggactgagtc gtgatattag
    47341 aaaagaactg gcctatgtga ttaatggcag gttcctgaga aaagatatta agaaagataa
    47401 aatcactgac cgggaaatgg aaattatccg catgacggct cagggaatgc tgcctaaatc
    47461 gattgccaga attgaaaatt gtagtgtgaa gacagtgtat acccatcggc gtaatgctga
    47521 ggccaagctg tactcaaaaa tatataagtt ggttccgtaa actccaggca agttagtttt
    47581 aaaaaatgac tcacagggac atcacgtcct caattcaact cgggaagaaa agcaatgaaa
    47641 aaaaaggttc tggcaatagc tctggtaacg gtgtttaccg gtacaggtgt ggcgcaggct
    47701 gctgacgtaa cagctcaggc tgtagcgacc tggtcagcaa cagccaaaaa agacaccacc
    47761 agtaagctgg ttgtgacgcc actcggtagc ctggcgttcc agtatgccga aggcattaaa
    47821 ggttttaact cacagaaagg tctatttgac gtggctatcg agggtgactc aacggctacc
    47881 gcctttaaac tgacctcacg tcttatcacc aacaccttaa cccagttgga tacctcaggt
    47941 tccacactga atgtgggcgt ggattataac ggcgcggcag tcgaaaaaac tggcgatacc
    48001 gtgatgatcg ataccgccaa cggcgtactg ggcggcaacc ttagcccact ggctaacggt
    48061 tacaatgcca gcaatcgtac caccgcacag gatggtttca ctttctccat catcagcggc
    48121 accaccaatg gtaccaccgc agtaaccgat tacagcactc taccggaagg catctggagc
    48181 ggcgacgtta gcgtacagtt cgacgcgacc tggaccagtt aagctctctg atgtaccagc
    48241 aggggtagcc cccctgcttt attcctggac ggaattgtta tgaaaaagca ccttctgcct
    48301 ctcgctctgc tgttttccgg aatatctccg gcccaggcgc tggatgtcgg cgatatatca
    48361 tcgtttatga acagtgacag cagcacgctg agcaaaacga tccaaaacag taccgacagt
    48421 ggccgcctca tcaatatccg tctcgaacgg ctctcttcac cgcttgacga cgggcaggtt
    48481 atcgcaatgg acaagccgga tgagttgcta ctcactcccg ctagcttgct gctacccgcc
    48541 caagccagcg aagtgatccg cttcttctat aagggaccag cagatgaaaa agagcgctac
    48601 taccgcattg tctggttcga tcaggccctc agtgatgcgc agcgcgataa tgccaaccgc
    48661 agcgctgtag ccactgcttc cgcccgcatc ggcaccattc tggtcgtcgc cccccgccag
    48721 gcgaactacc gctttcagta cgccaacggc tccctgacaa atacaggaaa tgcgacgctg
    48781 cggatcctcg cctacggccc ctgcctgaaa gccgccaatg gtaaggagtg taaagagaat
    48841 tactacctga tgccgggcaa gtcgcgtcgt tttacccgcg tggacacggc ggataacaaa
    48901 ggacgggttg cactttggca gggtgataag ttcattcccg tgaaatagat agctgtgcag
    48961 atggataacg acaatgcctt tacgacggtt ctccccagga ctgaaagccc agtttgcctt
    49021 cggcatggtc tttttgttcg ttcagcccga tgccagcgct gctgacataa gtgcgcagca
    49081 aataggtggg gtgattattc cgcaggcctt cagtcaggcg ctccaggacg gcatgagcgt
    49141 cccgctttat attcatctcg ccggtagcca gggtcgccag gacgatcagc gaatcggcag
    49201 cgcttttatc tggctggatg atggacagct acgcatccgg aaaatacagc tggaagagag
    49261 tgaagataac gccagtgtca gcgaacaaac tcgacagcag ctgatggctc tggcgaacgc
    49321 cccgttcaat gaggccctta ccatccccct gactgacaac gcgcagctgg atctcagctt
    49381 gcgccaactg ctgctgcagc tagtggtcaa gcgcgaagcg ctgggcaccg tactacgctc
    49441 acgtagcgaa gacatcgggc agtccagtgt taacaccctc agcagtaatc tgagctataa
    49501 cttcggcgtc tataacaacc agttgcgtaa cggcgggagc aacacatcca gctatctgtc
    49561 gctgaataac gttactgcac tgcgcgaaca tcacgtggtg ctcgacggct cgctgtacgg
    49621 gatcggtagc ggtcaacagg acagtgaatt atataaagcg atgtatgaac gcgattttgc
    49681 cggtcaccga tttgccggtg gaatgctcga cacctggaac ttgcagtcct tagggccgat
    49741 gaccgccatt tcagcaggga agatttacgg cctttcctgg ggaaaccagg ccagctccac
    49801 catcttcgac agcagccagt cagccacgcc agtgatcgcc tttttaccgg cggcgggcga
    49861 agtacatctc acccgtgatg ggcggctatt aagcgttcag aacttcacca tgggcaatca
    49921 tgaagtggat acccgaggtc taccgtacgg tatttacgat gtggaagttg aggtgatcgt
    49981 taacggtcgc gtgatcagca aacgcactca acgggtcaat aagctgttta gccgggggcg
    50041 cggcgtcggt gcaccactgg cgtggcagat atggggcggt agctttcata tggatcgctg
    50101 gtcggaaaac gggaaaaaga cgcgaccagc taaagagagt tggctggcag gtgcctcgac
    50161 ctccggctca ctgagtacgc ttagctgggc ggcaacggga tatggatacg ataatcaggc
    50221 agtgggcgaa acccgtctga cgctgccgct tgggggggcg atcaacgtta acctgcaaaa
    50281 catgctggcc agtgacagct catggagcaa catcgccagc atcagcgcca ctctacctgg
    50341 aggcttcagt tcgctgtggg ttaaccagga aaaaacccgc attggcaatc aattgcgacg
    50401 tagcgatgcc gacaaccgtg caatcggcgg cacactcaac ctgaactcac tgtggtcgaa
    50461 gctgggcaca ttcagcatca gctacaatga tgaccgccgt tacaacagcc attattacac
    50521 ggcagattac tatcaaaatg tctacagcgg tccctttggt tcgcttggcc tgcgggccgg
    50581 tattcagcgc tataacaacg gcgacagcag cgccaataca gggaaatata tcgctctcga
    50641 tctctcgcta ccactgggca actggtttag cgcagggatg acccatcaaa acggctacac
    50701 catggcaaac ctgtcagcac gcaaacagtt tgatgaagga accattcgca ctgttggtgc
    50761 caatctgtca cgagccatct ccggcgatac cggtgatgac aaaaccctca gcggtggggc
    50821 gtatgcacag ttcgacgctc gttacgccag cggaacgctg aacgtcaata gcgcggcgga
    50881 cggctacatc aataccaatt tgaccgccaa cggcagcgtc ggctggcaag gtaaaaacat
    50941 cgctgccagc gggcggaccg atggcaatgc tggggtgata ttcgacactg gcctggagaa
    51001 cgatggtcag atcagcgcca aaatcaacgg gcggattttc ccgcttaacg gcaagcgtaa
    51061 ctatctcccg ctctctccct atggaagata tgaggtggag ttacagaaca gcaaaaactc
    51121 actcgacagt tacgatatcg tcagcggtcg caaaagccat ctgactctct atccaggcaa
    51181 tgtcgctgtc attgagccag aggtgaagca gatggttacc gtctccggtc gtatccgtgc
    51241 ggaagacggc acactgctgg ctaacgcacg gattaacaac catatcggcc gaacccgaac
    51301 cgatgaaaac ggcgagtttg tcatggacgt ggataagaaa taccccacta tcgattttcg
    51361 ctacagtggc aataaaacct gcgaagtggc actggaactc aaccaggcgc gcggtgccgt
    51421 ctgggtcggt gatgtggtct gcagcggcct ctcatcgtgg gcggcggtga cgcagacagg
    51481 agaagagaat gagagttaac ctactgatag cgatgataat cgttgcgcta atctggcctg
    51541 caaccgcgct cagagcggca gtgagcaaaa ccacctgggc ggatgctccg gcacgcgagt
    51601 ttgtgtttgt cgaaaacaac tcagacgaca actttttcgt cactcctggc ggggcgctgg
    51661 atccgcgcct gaccggtgcc aaccgctgga ccggtttaaa atacaatggt tcaggaacca
    51721 tctatcagca aagcctcggc tacattgata acggttacaa caccggcctt tataccaact
    51781 ggaagtttga tatgtggctg gaaaattcac cagtttcatc tcctttaact ggcttgcgct
    51841 gcatcaactg gtacgctggg tgtaatatga ccaccagtct tatcctgccg caaaccaccg
    51901 acgccagtgg attttatggc gcgacggtga ccagcggcgg cgcgaagtgg atgcacggca
    51961 tgttgtcaga cgccttttac cagtatctgc aacaaatgcc cgtcggcagc agctttacaa
    52021 tgaccatcaa tgcctgccag acctctgtga actatgacgc cagcagcggc gcacgctgta
    52081 aggatcaggc ctccggcaac tggtatgttc gcaacgtcac ccatacgaaa gcagcaaatc
    52141 tacggttgat aaatacccac tcgctggcgg aggtgtttat caacagcgac ggagtaccga
    52201 ctctgggcga agggaacgcc gactgccgga cgcaaaccat cggcagccgt tcaggattaa
    52261 gttgtaagat ggttaactat accctgcaaa caaacggact cagcaacacc tcaatccata
    52321 tattcccggc gatcgccaac tcgtcgttag cctcggccgt cggggcgtac gatatgcagt
    52381 tcagtctgaa tggcagttca tggaaaccgg tgagcaatac tgcctattac tacaccttca
    52441 acgagatgaa gagcgcagac tcgatctatg ttttcttctc gagcaacttc tttaagcaga
    52501 tggtgaacct cgggatcagc gatatcaaca ccaaagatct attcaacttt cgctttcaga
    52561 acaccacatc accggagtct ggctggtatg aattttctac ctccaacacg ctgattatca
    52621 aaccccgtga tttcagcatc agtattatct ccgatgaata tactcagaca ccgtcgcggg
    52681 agggatatgt tggcagcggc gagtcggcac tcgatttcgg ctatatcgta accaccagcg
    52741 gtaaaacagc tgccgacgaa gtgctgatca aggtgaccgg acccgcgcag gtgattggcg
    52801 ggcgctccta ttgtgtcttc agctccgatg atggtaaggc gaaagtaccg ttcccggcga
    52861 cgctttcctt tattacccgc agcggagcta caaaaaccta cgatgccggg tgcgatgata
    52921 gctggcggga tatgaccgat gcgctgtggc tgaccacacc gtggactgat atctctggcg
    52981 aagtggggca gatggataag accacagtca aattttcgat tccaatggat aacgccattt
    53041 cgctgcgtac ggtagatgat aacggctggt ttggcgaagt cagcgcttca ggagagattc
    53101 atgttcaggc gacgtggcgt aacattaact aaggccctgc tgacagtggt ctgtatgctg
    53161 gcggcacctt tgacacaggc gatttcggtc ggcaatctga ctttttcact gccgtccgag
    53221 actgactttg tcagcaaacg tgtagtgaat aacaacaaaa gcgcacggat ataccgtatt
    53281 gccatcagtg ctattgatag cccgggcagc agtgaattgc gcacccgacc ggtggatggt
    53341 gaactgcttt tcgccccccg ccagctggcg ttgcaggctg gtgagagcga gtattttaaa
    53401 ttttactatc atggtccacg ggataaccgc gagcgctact accgggtctc atttcgcgag
    53461 gtccccactc gtaaccagac aagacgtagc ccaaccggcg gcgaggtcag cacggagccg
    53521 gtggtggtga tagataccat tctggtagta cgaccacgtc aggttcagtt taaatggtcc
    53581 ttcgaccagg tgacaggaac ggtaagtaac actggcaaca catggtttaa gctactgatt
    53641 aagccaggat gtgattcgac cgaagaggaa ggcgatgcct ggtatctacg tccgggagac
    53701 gtggttcatc agcctgagtt acgtcagccg gggaatcatt atctggtcta taacgacaaa
    53761 ttcattaaga ttagcgattc ctgtcctgct aagccccctt cggcggacta agacttatcc
    53821 atcggacgag ggaagaattc cacctcagag actcaaataa tcgattaaat gatgtggaag
    53881 cgatcgtcaa tgtcacctgt aacgatgggc tttctcatat gtacaccata gtgttcagga
    53941 gacacggaaa ggtaccaggc agcctgctaa cgagtggttt attcacgaat tagtacgtaa
    54001 aatcggtaac ggctggaaat cattcaatac tcacactatc gaaagttccc cagccaaccg
    54061 cggtacgttc ttacatacga tgtaccgctg ttctctttac gatttatagc tgtactggtg
    54121 aattatgagc aatctgaatc catgcatgac gtgtggtgcc tgttgtgcat ttttccgcgt
    54181 ctctttttac tgggccgaag ccgacgatgc tggcggtacc attcccgcca ggctcactga
    54241 acaaatatcc ccttttcacc gatgcatgag cggtaccaat cagaaaaacc cccgatgtat
    54301 tgcccttgca ggaacaccgg gcaaaaatgc ctgctgcacg atatataaaa atcgatcgtc
    54361 cacgtgcaga gaattcgcca tgtctggtga gaacggagtc gtcaatgagg cttgcaatcg
    54421 tgcaagagct aaatacgggc tgacaccgtt ataaatatgc aataattaat tgcactgccc
    54481 cgccagctat aaccgcgggg cttcgttttt cagggataac aaaacccgaa acttcttcca
    54541 tctgccagca acaatgcatc ttcctctata gtgttctccg gatctacagc atcctgaagt
    54601 agcgcgatgt actcactggc tatctgcttc agaaagtaaa aagcaatgtt tttaacctat
    54661 aaaaatggcg ctgcatttgc gccattttta ttattcaatg cattatctgt ttgagcctaa
    54721 agggatctca gggtctggct catgagtaat tctgtttcgt aaatctctgc gaataatttc
    54781 aatggaccag aaccagacta aatgtccaaa aatttcagaa acattctcat accacgggag
    54841 atcaaacaga ggtggcgtca gtcccatgag agggaatgaa atcatatgaa caaaaagttg
    54901 ggctaaagca cctgccagta agccctgcca gagtttaatt tttggaaata cttcagcgac
    54961 cacacagtaa ccaacggcaa acactatcga aaagataatg tgcgtaacgc caacccagtt
    55021 aaagacatgc ccggcaaagg tataaacagc cgcattggga tctgtcagcc ctagccagtc
    55081 tcgaagaaaa atatacggag gattgagaaa attacgggag caatcaattt gcccagcagc
    55141 cctgattaat gattccgggc cacacgctgc attaaacata tccaccgggc tacgtggcgg
    55201 caatggaact tcagcccccc acttcacaaa tgcggaaaca acgccagcaa tcagcccaat
    55261 gaatgcagca agaccataac gtctgcggtt cggtggagtt tgttcaaata tattcatatc
    55321 taccctgctt gtaccattat gttatacacc tcttcaggag tattcataaa acaagacaaa
    55381 tgtaaagaac tgtattgttt tgtataacaa gatagtttcc taatcgccaa tgaatataag
    55441 caccatcatt tctccctatt tttatgttag aagtgacaaa gatttgtagg gtgatgcaga
    55501 gctgacaatc acaggtttcc ttatcggttt ttgcatcgta caactaaagc aataaacgag
    55561 ctcccgtcat cggcgaatat gaattaatta gccgggctca tagctgcatt cgcgttttaa
    55621 gaataatcct cctgctctca ccgactatgc ttaacgttta aaaaagcatc agcactctcg
    55681 caacgctctc ttattttccc ctttagaata ccggaggcct ggtatgagca accaaggcga
    55741 ataccccgaa gataatcggg ttgggaagca cgagccgcac gattttagtt tgacccgtcg
    55801 cgatctgatt aaagtcagcg ccgcaacggc ggcgaccgcc gtggtttatc ctcattctac
    55861 gcttgcagca agcgttccgg cagctacacc cgcgccagag ataatgcccc tgacacttaa
    55921 ggtgaacggc aaaaccgagc agttggacgt tgatacccga accacgctac tggacgcttt
    55981 gcgtgaaaat ctgcatctga tcggtaccaa gaaaggttgc gatcacgggc agtgcggagc
    56041 ctgtaccgtg ctggtcaatg gtcgcaggct taatgcctgc ctgacgcttg cagtcatgca
    56101 tcagggggcc gagatcacca ccattgaagg cctgggttca ccagagaatc ttcaccccat
    56161 gcaggcggcc tttatcaagc atgatggctt ccagtgcggc tactgcacct ccgggcaaat
    56221 ttgctcatca gtagcggtgc taaaagagat tcaggacggc attcccagtc acgtcacggt
    56281 cgatttggtt tcccctccag aaagaactgc cgatgagatc cgtgaacgta tgagcggcaa
    56341 catctgtcgc tgtggtgcat acgctaacat ccttgccgcc attgaagatg ctgcggggga
    56401 gataaaatca tgaaggcgtt tacctatgaa cgagtgaaca ccccggccga ggcggcactt
    56461 agcgctcagc gcgtacccgg cgcaaaattt atcgctggcg ggaccaatct gctggacctg
    56521 atgaagctgg aaattgaaac gccaacccac cttatcgatg tgaacgggct cgggctcgat
    56581 aagattgaag tgaccgacgc gggtgggctg cgcatcggcg cactggtatg gaacaccgac
    56641 ctggcagctc acgagcgcgt gcgtcgtgat tacgcggtac tctcccgcgc cctgctcgct
    56701 ggcgcgtctg gtcagttacg taatcaggca accaccgcag gtaatctgct ccagcgcgcg
    56761 cgctgcccct atttttacga caccaatcag ccctgcaata agcgcctgcc cgggagcggc
    56821 tgcgcggcac ttgaaggctt tagccgtcag cacgcggtgg taggcgtaag cgaagcctgc
    56881 attgccaccc atccgagcga tatggcggtc gcaatgcggt tgctggatgc ggtggtggaa
    56941 accatcacgc cggagggaaa gactcgcagt atcacactgg ctgattttta tcaccctccg
    57001 ggaaaaacgc cgcacattga aaccgccctg cttcccggtg agcttatcgt tgcggtgacg
    57061 ttacctccgc cgctcggcgg aaaacatatt taccgtaagg tgcgcgatcg cgcctcctac
    57121 gcctttgccc tggtatcggt cgcagcgatt attcagccta acggcagcgg gcgcgtcgcg
    57181 ctgggcggag tagcacataa gccctggcgc attgaggctg cggatgctca gctatcccag
    57241 ggggcgcagg ccgtgtatga cgcgctgttc gccagcgccc atcccaccac tgaaaacacc
    57301 tttaaactcc tgttggcgaa gcgaacgctt gcctccgtac tggctgaagc gagggcacag
    57361 gcatgaaatt tgataaaccc gcaggggaaa acccgattga tcagctgaag gttgtcggtc
    57421 gtccccatga ccgcatcgac ggaccgctga aaactaccgg cacggcacgc tacgcctacg
    57481 aatggcatga agaagccccc aacgccgcct atggttatat cgtcggttcc gccattgcca
    57541 aaggatgcct caccgccctc gatacggacg ccgcgcaaaa agcgccgggc gtactggctg
    57601 tcattaccgc cagtaacgcc ggggcactcg gcaaaggcga caaaaacacc gccaggctgt
    57661 taggcggccc cactattgag cactatcatc aggccattgc gctggtagtg gccgagacct
    57721 tcgaacaggc acgagcggcg gcctcgctgg tgcaggcgca ctatcgccgt aataaaggag
    57781 cttactccct ggcggacgag aaacaggccg tcagtcagcc gccggaagac acgcccgata
    57841 aaaatgttgg tgactttgac ggggctttca cctccgcggc ggtgaagatt gatgctacct
    57901 acacgacccc ggaccagagc catatggcga tggaaccgca tgcctcgatg gccgtctggg
    57961 atggaaataa gcttaccctc tggacctcaa atcagatgat tgactggtgc cgcaccgatc
    58021 tggcaaaaac gctgaaagtg cccgtggaga atgtgcgtat tatctccccg tatatcggtg
    58081 gagggtttgg cggcaagctg ttcctgagaa gcgatgcgct gctggcggcc ctcgccgccc
    58141 gtgcggtgaa acgtccggtt aaagtgatgc tcccccgccc cactattccc aataacacaa
    58201 cgcaccgccc cgccaccctt cagcacctgc gtatcggtgc cgaccagagc gggaaaatca
    58261 ctgctatctc acatgaaagc tggtccggaa acctgcccgg cggcacgccg gaaacggcgg
    58321 tacagcaaag cgaatttctt tacgcagggg cgaatcgtca tacaggcctg cggctcgcca
    58381 cgcttgattt gccggaaggg aacgccatgc gtgcgcccgg cgaagccccc ggtctgatgg
    58441 cgctcgaaat cgcgatcgac gaactggcgg aaaaagcggg catcgatccc gtcgagtttc
    58501 gcatcctgaa tgacactcag gttgaccccg ccgacccgac gcgccgcttc tctcgccgtc
    58561 agcttatcga gtgcttgcgc accggagcgg ataaatttgg ctggaagcag cgcaacgcca
    58621 cacccggaca ggtacgcgac ggggagtggc tagtcggcca cggtgttgcg gcgggctttc
    58681 gcaataatct gctggaaaaa tcgggtgcgc gggttcacct ggagcaaaac ggcaccgtta
    58741 ccgtggaaac ggacatgacc gatattggca ctggcagcta caccattctg gcccagacgg
    58801 cagcggaaat gcttggcgta ccgctggagc aggttgcggt tcacctcggc gattccagtt
    58861 tcccggtttc tgcgggttct ggtggacaat ggggcgcgaa tacctccacc tccggcgttt
    58921 acgccgcctg tgtgaagctt cgcgaaatga ttgcctcggc agtcgggttt gatcctgagc
    58981 agtcgcagtt tgccgacggc aagattacca acggtacccg aagcgccatg ctacatgagg
    59041 ccaccgcagg cggcagactg acagcggaag agagcattga attcggaaca ctgagcaagg
    59101 agtaccagca atcgaccttt gccgggcatt ttgtggaggt cggcgtgcat agcgcgacgg
    59161 gagaagttcg ggtccggcgt atgctcgccg tgtgcgcagc ggggcgcatt ctgaatccga
    59221 aaactgcacg cagccaggtc attggcgcga tgactatggg catgggcgcg gcactgatgg
    59281 aggagctggc ggtagatgac cgtttgggct acttcgttaa tcacgatatg gcggggtatg
    59341 aggtgccggt tcatgcggat atcccaaaac aggaggtgat tttcctggat gataccgacc
    59401 ccatctcctc cccgatgaag gccaaaggtg tcggtgagct gggcctgtgc ggcgtgagcg
    59461 cggctatcgc caacgcggtg tataacgcca ccggtattcg ggtacgggat tatcccatca
    59521 ctctggataa gttgctcgat aagctgccgg atgtggttta aggaggaaca atgtcatact
    59581 cgctttttga caaagacgag cactggcata agccagagca ggcgtttctc accgatgacc
    59641 accggaccat tctgcgcttc gccgtagagg cgctaatgtc cggtaaagga gcggtgctgg
    59701 tgacgctggt ggaaatacgc ggcggcgcag cgcgcccgct cggggctcag atggtggtgc
    59761 gcgaagatgg tcgttattgc ggttttgtct ctggcggctg cgtggaggcc gcagccgctt
    59821 ttgaagcgct ggagatgatg ggctcaggcc gcgatcgcga aattcgctat ggcgaaggtt
    59881 cgccgtggtt tgacatcgtt ctgccctgcg gcggtgggat cacgctgacg ctccataaac
    59941 tacgctcggc acagcctctg ctcgccgtgc tgaaccgcct ggaacagaga aaaccggcgg
    60001 gtctgcgcta cgatccgcaa gcacaatcgt tggtgtgcct gcccacgcaa acccggacgg
    60061 gctggaatct caatggcttt gaggtggggt tcaggccatg cgtcaggctg atgatttacg
    60121 gacgttctct tgaggcgcag gcaaccgcga gtcttgcagc agccacaggc tatgacagcc
    60181 atatcttcga tctttttccg gcctcagcca gcgctcagat cgataccgat acggcggtca
    60241 ttttgctgtg ccatgatctc aaccgggagc tgccagtgtt gcaggccgcg cgagaagcaa
    60301 aaccctttta tctcggcgca ttgggcagct atcgaaccca cactttacgt ctgcaaaagc
    60361 tccacgagct gggatggtcc agggaggaga cagcgcaaat ccgggcaccc gtcgggatat
    60421 tccccaaagc ccgggatgcg catactctgg cactctccgt gctggcagaa gtcgccgctg
    60481 tacgtctccg tcaggaggat tcatgcctgc ctccatcgtc ctgatcctcg cggcggggcg
    60541 tggggagcgc ttcctcacct ccgggggaaa tacccataaa ttaagagaaa aatctgcagt
    60601 agccgtttaa gaaaatcacc taaatcgttt aaaagagcgc ccaacccata gagggcgctt
    60661 atcgccttgt gtaacttatt cagcaacgcg cgcctcgccg gactaaagcg caccggcctt
    60721 ctggcgtgcg gtaacaaagg tcagtacgcc agcgcaaagg agcagcacgc tactcatagc
    60781 aaacgtgctc tgccagccaa ggcggtcaaa cacaatgcct cctgccgttg atccaagggc
    60841 gatggcgagc tgaatcaccg cgaccatgag cccgcctcca gcttcggcgt tgtcaggcaa
    60901 cgtacgcgca atccatgtcc accatccggt tggcgcggcg gtggccagca tgccccagag
    60961 tcctaacagc agcgagaccg tccagacgtg atgcccggta agcatcagac cgatggcaat
    61021 gacggccatc agtccgggta tggccatcag cgtggggtag aattttctgt tgagaaatgc
    61081 cgaaacaacc agcgtgccaa taaaaccggc cacgccgata atcagcaaaa tcgacgacaa
    61141 gcctaaggaa ttaacccgtg tcaccgactc cagaaacggg cgcacatagg tgaatagtgc
    61201 aaactgcccc atgaaaaaca ggccgcaggc tatcatgccg accacaacca tgcggcggct
    61261 aaataagcgg aataccgcgc cacatgacgt gtggctttta ttcgcatcca tgcttggcag
    61321 actaatgcat tgccagataa aggcgacaac cgcgataggc accaggcaca agaaagcgcc
    61381 tcgccatccg accgtagccc ccagatagct tcccagcggt gcggccacgg ccgtcgccag
    61441 cgcattaccg gcgttgaaaa tcgccagcgc acgcgtaacc tgatgctgcg gtaccagacg
    61501 aatagccgtt gccgctgaca tcgaccagaa tccgccaatg gccacaccaa tcatcgcacg
    61561 accgaccata tacatcagat aactggaagc cagcgcaata ataagccccg atacggccat
    61621 cagaaccgtc atccccagca gcaggaactt acgattcatc tttccagcta gcgttgaaag
    61681 cgtcaggctg gtcagaaccg ccagcgcgcc ggagatagca atcccctgcc ctgccagccc
    61741 ctccgtcacg cctaaatcgc gggtaatcgg cgtgagcagg ctgacgggca taaattcaga
    61801 agcaatcagc acaaacacgc acagggtcat ggcaaaaata ccgccccagt atgcgcgctg
    61861 atgatgaggt gatagtgttt ggcttagtgt cgacatagtg aaatttcaca aagatcagga
    61921 taaaagccac cagcctatgg ccgatggctt tgcatcaatt caggagaggt tttatttcag
    61981 tttggtctgg aaaaattgtt cgaacttcgc gaaaggtatt tttccggcca cgttatcgta
    62041 caggtccacg tgatttgccc ctggtaccac caccaactct ttgtctttac tgccaaccgc
    62101 cttgtaagca tcttcagcaa aatagcgtga atgtgctttc tcaccggtca caatcagcgt
    62161 cggaatggtg atttcactgg cgtagctcaa cagcggcata ttcataaatg acagcggcat
    62221 cgttgccgtc cacgccccgg tagagttaac ggaacgttcg tggaagccgc gcggcatgcg
    62281 atagtagtca aagaactctt tcagtaccgg atgaggattt gccggcagcg tttctggcag
    62341 aatgcgctcg cccgcactga ccttgccgtt gctatcgaca ttaatatcat gaccaccgtg
    62401 agcgaacgtt ccgctctgcg catccttcca gcgctgttca ttcagatatt gcagaacggc
    62461 acgacggtcg gcggtagaat aacggtcttt gccatccccc acaccatgac ccatcgcccg
    62521 gctcatgtca tacatcacgc tggtagctac cgctttaacg cgggtatcca tcgccacgtc
    62581 gttcagcgcc atgccgcccc agccacaaat accgagcagc ccgatacggt tgcgatctac
    62641 ctctttttgc agccctaaga aatccaccgc cgcgctgaaa tcttcagtgt tgatatccgg
    62701 tgaggcgacg tttcgcggat agccgccgct ttccccggtg taagaaggat cgaacgccag
    62761 ggtaacaaac cctcgttccg ccagcgtctg cgcatacaag ccgctggatt gctctttcac
    62821 cgcgccaaat ggcccactga ccgcaatcgc cgccagcttg cgatcgccac ggtctttagg
    62881 caggtaaaga tcgcccacta aggtgatgcc atatcggttc gggaacgaga ctttacggtg
    62941 atcgactttc tggctctcgg cgaacgtttt atcccattta tctgtcatgg acacaggggc
    63001 attaggattg gttgaatcag cataactcat tgtcgtgact ccacttaatg atgcgaaaag
    63061 cagcatggtc ggcatcgcgg tttttagcgt gcgggtgaag gctttcataa aagtccccat
    63121 aaaaaacaac agagagatac aggtttccga cggacaacgc tgcggtgctt tactttgatg
    63181 gggtcattat agtgagcaga attagtgctg attagggggc gattaatgct aagattaata
    63241 ccatattgtt ataaattaat gcggagattg ccggcatgaa ggggggatgt ttcaatggcg
    63301 aaaagggaga actacaacga gctgtacctg tttatgcagg tggtgcggga aggcagcttt
    63361 accgcagcgg ctcagcggct gggtcttgct cagtcaggag tcagccgttc tgttcgcgag
    63421 cttgaagaaa ggctgggcgt ccagctgcta gtgcgcacca cgcgtaaact gtcgctgaca
    63481 caagcaggcg agcagctcta ccagaagacg gcatctggat ttgaaatgtt agatcttggg
    63541 cttgccacgc tggcgcacta tcgagagacg ccctccggca cggtgcgtat caatgccagc
    63601 cagcacgcga ttgataaatg cctgctgcca aagctggcgg tatttaaaca gagatatcct
    63661 gatatccggc ttgaactgat aaatgagagc cggttcgtcg atatcatcga ggaaagattc
    63721 gatgcaggtg tccgcctggg gcctgaagtc ggccagggta tggtcgcggt acgcatcacg
    63781 cccgatatgg agatggcaat tgtggggact ccggaacatt ttcgtcgcta tggttttccg
    63841 caaaccccgg cggatttaaa ggcgcatccc tgtatcgcct atcagtttgc cgacggcagc
    63901 gtataccagt gggaactcaa tcaggatgat aaaaaaatca ctcatcgccc tgaagggcaa
    63961 tgggccttat ccgacagcta tatggaagca gaagcagccc ggctgggcct cggattggcc
    64021 tatgttcctg ttgaactggt cgcggatgat ttagaacacg gtaagcttat cagggtgttg
    64081 cagcgttaca gcctgcgaat ggagggattg tttctctatt accctcatcg caacgtctcc
    64141 cccgccctgc ggatggtcat tgatacattg aaaatctgaa cgttaataac gaccgactct
    64201 tcactgtgca aaattgtgtc aggtagtccc tctgcgttca attccagggt ctgctttgag
    64261 cgagaaagag gggactatcc agcaatggag atcacccaaa agaaaaaacc acctggctcc
    64321 ctcgacaacc ttcatccggg catttagcct caatcaacat ttttatatct atcaggttac
    64381 ttcattatga aaactgatca ttatcgttgt ttttgttgca atagtagaat acgcgtattt
    64441 attcctccat ctctattaat aacggcaata aactgttcac ttcactgcta tttaaaatat
    64501 acatcctttt caagctgttc agcggcgtaa gaaaaagtat taattatgaa ttcgacgatt
    64561 taaccatttc tggctgattg gtaatcatct gtgattaatg ctggtctatt gattacgcca
    64621 gcaccctctc tagcttattc accgccgcca catacttcgc cggattttta tctttcagca
    64681 ccgccagatt acgttgcttc cagcgaatag ccggaagatg ctgaatttca ggatagcgat
    64741 accgcgacca gtcagggctg ttttgcttaa aactgagcaa gaactcgcga tccagatccg
    64801 taagatgtga cttcaacgcc tgcggcagca aagtcgttac tgacagcagc gattccagcg
    64861 acgtttcctg ctgagtcatc cccgaaaatt cctgagcata cagcgtcgtg atccgctctg
    64921 tatcccagtt tggggccagc aactcggcaa tggggcgggg atggcctgcc agataacaaa
    64981 gaaaaccgtc aaagatctcc cgcatcagtc ccggtttctc cagcatattc agcacatcaa
    65041 acagatcgcg cgggtgctga cgatcaagcg ctgcgcagat tttaccgcca taaagatccg
    65101 ccagcgagac gacactcatg gtggtgaagc catactccat ttccacacgt tcgcacacag
    65161 gcatttctgc cggaggcaac aacagcccgc gccagaccgg gctaacctcg atcttaatct
    65221 ttgcatcagc ggtattcaca atgatgcgct tttcatccgc tttattttcc tgccgaatcg
    65281 cggtgatccc cggtctgccg ttcagcgatg cagtaatcct cttcaaagcg tcgttgatag
    65341 ccgccagatc agtatcgcga tccgcaaagc tcttatacgc cagatcaata tccaccgaca
    65401 gacggggaaa atcctgcaca aacaaattga ttgcggtgcc gcctttcaac gcgaaacagg
    65461 actccttcgc cacatacggc agagcggcca tcaacagttt gacctgccgg gcatagctat
    65521 cacgcagatc catcttcgac tccttccggt ttaaatgcgc gtggaacagt aatttgataa
    65581 tccggctcca gccagccgcc gggtatgatt tgccgctttc ccgtacctgt ttctataccg
    65641 ctttgatcaa taaactgaaa ataagggtgt ccattacgcc gggcaaggta aagcatcacc
    65701 cggttggttt taaccgaatg actggcgcag agtaacgcct gcaatttacg cgggctgaga
    65761 agattcatcc cctgaaacag agcatcggca tgttcgaacg agatcaacga aggaacgcca
    65821 caagcgatct catacgcgac cagttccggg acactgcccg ctaactgccg gtcaccaatt
    65881 ttcagttccg tagtaaatgg cgttacatca gcggtaaggc ctgacgtata cagcgtgaca
    65941 aatgttgcgg attgttcaaa ggcagaaaac catcccggca gtttgacgta agccggaagc
    66001 gcgagccaga cctgcggctt accctgttcg gtatagtgcg catgaccctg aagcgcaagg
    66061 ctggttagcc ccgccacccg aacaggcttt tcccactgcg attgcaggca gtaaaccgca
    66121 tccacccaat ccggttcacg acccgtccgg cagtatacgc cgtgagcgat ccgcctgagc
    66181 cagccattct tcacatagag ataagagagc ttacgatcta tgccgttgcg ggtaagccat
    66241 gactggagca gcacctggcc gggtagcgtg tgctgcatca gccagtttag atagtttggc
    66301 aactgtcgcc tctgaataga cattagtaca aatatttaaa ccagtgtaga ttcagggata
    66361 cgccacagca agaaatagtt tacttattcc tcattttgtc gactaaaaat cgacattaaa
    66421 caaaaaattc aaacttaatc actttcggga aaaatgtgac aaatatatgc tcggactggt
    66481 tgcggggagc gtgtaacatg gatacaaatc aaaattattg ccagcctcac tgatggatta
    66541 ctggtgtcaa gagcccccct tcgggcatga aacggctggc taattctgta cagactgtaa
    66601 tctaaggacg ataacgcatg acatatcagg caattttcac tggctgggat gatctgacga
    66661 ttgaagacct tctggtcgct taccggaaag caaaagccga tagcttcttt gagaatacat
    66721 ttcctgttgc tatcaaattt gccgagtatg agcaggaatt acttgaaaac ctgcaaaaac
    66781 tcttagatct tttgcagagc gaagatggat tcagtagcaa taagaagttg attggcaaat
    66841 ttcgtttgtt accgaaaaaa ttaaccacaa agaaaaaaca tgaatcccaa aatggacacg
    66901 tccacttttc taatcctaaa cgagcagccg accatttatt taataatttt gatctgatac
    66961 cagagtttcg tattattggt gacttcccgg ttgatagtca cattatctct gcactatgga
    67021 ttaacatggt cgggcataaa tttgatgcca gcttagataa ctgttgctat ggcgcgcggc
    67081 taaagcgtat tcgtaatgat gaattattta gcaatgagca ggataatcca ttccatatca
    67141 gtgccgtggg ttcttttagc ccctacttcc agccctacca aaaatggcgt ggtgatggct
    67201 taaaagctat acgtgacgag ttggaaaaag atcgtgacat tatcgccgcc tcactggatt
    67261 taaaaagtta ctatcatttt attgatccac tggctataac ctctgatgat ctctataaca
    67321 cactaaacat aaaactgact gaggatgaaa aagcgtttac tgcacagtta gcagtattct
    67381 taaagcactg gtctgacggc gcagcggcat ttggaaagaa aatagcgtac aaaacacctg
    67441 ttattaatgg tggtctggtc attggattaa cagccagtcg gatcatttca aatatattgc
    67501 tacaccattg ggataaatta gtcattgaaa aactatcacc aattcactac ggtcgttatg
    67561 tcgatgatat gttccttgta atacgcgata cagggacaat tactaataat cacgaattta
    67621 tgttattgct gcaagatagg cttggcaatg attgcgttta tttgaaaaac gagcaaaaac
    67681 aaatatggca aatacagcag ggcgagcatt tccagggtaa gaccaccatc cagttacaat
    67741 ccgataagca aaaacttttc gtgcttcaag ggagggctgg aatagacctg ctcgacagta
    67801 tcgaaaagga gatctacgag ctttctagtg aacaccgctt gatgccttca ccggatcaac
    67861 tggaacactc caccgcagct aaagtccttt ccgctgccgg tagtgtaggt gaaaatgccg
    67921 atactctgcg ccgtgcggat ggattaacca ttcgtcgttt gggctggtca ctgcaattac
    67981 gctacgttga aacactggca cgagatctgc ctccaagtga atggaaagaa cagcgggaag
    68041 agttttatca gtttgcctac aaccatattc ttagggctga taatctattt gcacatttta
    68101 gttatctgcc aaggctgctt ggctttgcta tcagtatgaa tgaatggcag cacgcggaaa
    68161 aaattgtact taaagcttac gaatccatca acctgttggc atcggtgatt acttcaggta
    68221 aggaagtgaa tataaatggt tgcaaaactc gagcagtaaa tgatctttgg cgctgtataa
    68281 aaggcacatt aagctggcta tttgttgatg cagcgacacg atattacagt cctgacagat
    68341 tatttcttga taaacgttca aagaaagaag agtgccttgc ggatacattt tttaatcata
    68401 tttcacaaag tctgacgaat ctaaaggatt tactggatct tcgctttgat tcagcagatt
    68461 tttatttaaa agcgccattg gtagctcgag ctgatttagc aaaggaacct tataaacaga
    68521 tcgtaaagag tcagtcggca gaaaaacttg ttaatcagcg tgatagtaaa aaagaagtta
    68581 aaatactgaa attaatgagc gactcatcgc ttattgatat tgacgttatt aagctatttt
    68641 tgaaatcaac caagaatacc cgactggaaa aagtggctaa aggaaatcgt aagaacgaaa
    68701 gttacctacc ttacattttc cctacacgtc ctttaacacc cgctgaaata tcagaactgg
    68761 cccccgaatg tgttggatta ccctccacat ccgacaaaaa accagatgag agaccgtcca
    68821 ccatttgggc aaaatatact caagcattac gcggagtatg gatcaaaccg acgttgctag
    68881 catcggagca ggactcagat gaagcgacaa aaaaagctcg gcctaagaaa ttcattcata
    68941 ttggcacaga caggaaacat aaagttgtcg ttgcgctaac cagcattaaa acagaggagg
    69001 acgactgggc taaaatggcc tgcaataaat ctaacttgtc ccgttcaagg taccagcgga
    69061 tttctgaact ggttaatgca acattgaaac tatctcctaa acctgattat gttttattcc
    69121 ctgagctttc aatcccgtta cgctgggtta acagtattgc tgatcgtttg agttcggcgg
    69181 gtatcagtct aattgcggga acagaatacc gccacttaga cgataatcaa ctgaagagtg
    69241 aggccgtact tgtcctttca gataacagac tcggctatcc agcgagtgtc aaaatatggc
    69301 aacccaagct ggaacccgcc gtaggtgaag atgaggcatt attttcaatt tatggtaagt
    69361 cttgggattc gacacttaat gttaaacaac gtaagccggt atatattcat cacggcgtca
    69421 attttggcgt tatgatttgc tctgaactcc agaatagtaa agcgaggatc cgttttcagg
    69481 gcgcactcga tgcattaatg gtattgagct ggaataaaga tctagatacg tttgcatcgt
    69541 tgattgaatc agcagcgctg gatattcatg cctatactat tttagtgaat aaccgaaaat
    69601 acggcgatag tcgcgtacgt tccccggcaa aagaaccctt tatgcgtgat attgctcgtg
    69661 tgaagggcgg tgataatgac tttgtggtcg ctgcaacgct ggatatcgac tcgttaaggg
    69721 catttcagag cagggcaaaa cgctggccta aaggcggcga taaattcaaa ccgttacctg
    69781 aaggattcca gttggcaaag aaccgcaaaa agctaccgcc aaaataagaa actgattttc
    69841 gctattaata atcagggtat ttttgcgtga gatgttggta aacatgatgt agcccttgcc
    69901 actcatgacc aatcgcagta tctttctccc gcgcctgcaa aatcaggcgt cgggattagc
    69961 ctcctgaaga aatcttatcg gcgacacatg acgcgccagc gtcttttttt gtgttgttcg
    70021 cacggttaca tctcaatggt gggccgggcg ggggcgtcgc aagacgcgcc agtgtccgat
    70081 aaggctggta aggctaaccc tgcccggttc caccacccgc gagattagcc tctccggtgg
    70141 tggcttgaac acccacttat cggaggttgc cctatggcta caatcctcac cactgacagc
    70201 gatttactcg atctattgac cgtccccttc aatgccacca cggatttcac tgtccttgct
    70261 gactactgcg cccgctttgc cgaaaccctg attgaaaccg acgatccagc gctacgactg
    70321 gcgctctgcg ggcggcttca cgcggctctt gcgctactgc aacccacact acacgacccc
    70381 atcccaccgc atcttaccga aagcctgacc gtggataccc ttcccgccac accagagcac
    70441 tttatttccg aacctgattc cttgtgcgaa tactgcctcg cactactcca gcttctgctg
    70501 gaacgtccgg cggaaccaaa aatggagcaa accctgaccg ggctgctgtg tgagctggtc
    70561 tggtatttca cggaaggact caaagcccca cgctggctgc gtaccgccga cggcgtaaaa
    70621 tttatcagcg aggtggccgc atgatgcaac aggactggca ttccgccgac attattgcgg
    70681 tgctgaaaaa gcggggaacg cctctggcgg cacagagaaa aactcattgc ggaagcgctg
    70741 taaatatcgc cagaaatcat ctggccttca aggtactttg atagcaaagg caagcaggct
    70801 ggttcagtcc agtgtttctc ttcttgcatt ttcagaaatg ggaggctggg agtctatcga
    70861 aatggttcgt cgatatgctc acctcgcgcc taatcacctt accgaacacg cacggcaaat
    70921 agactcagtc ctgaacccat cggtcccaaa tttgtcccag tcaaaaaata aggaaggtac
    70981 taatgatgtg taacttattg attttaatgg tgccgataag aggagtcgaa cctccgacct
    71041 tcgcattacg aatgcgctgc tctaccaact gagctatatc ggccctgaaa ggacatgttc
    71101 acgaacgtga atcacggtgg acaaggttaa aactaaccgg gcgatgcgtc aatggccttg
    71161 tgaatcaaat ggctactttt gcatcacccg gttttattta cgcacgaatg gtgtaatcac
    71221 caatgccgat ccacttgtaa gtggtcagtg cttccagccc cattgggcca cgcgcgtgga
    71281 gtttttgtgt gcttaccgcc acttccgcac ccagtccaaa ctggccgccg tcggtaaaac
    71341 gcgtagaggc gttaacgtaa acagcggacg aatccacttc gttaacaaaa cgctgggcgt
    71401 tgcgcatatc gcgggtcagg atcgcatcgg agtgttgtgt gccgtgttca cgaatatggg
    71461 cgatggcatc gtcaagatcg ctgacgattt tgacgttcaa atctaatgac agaaactcat
    71521 cgtcatactc ttcggcttta acagccacca ccttcgcggg gcctgcctgc aactgcgcca
    71581 gcgcagctac atctgcgtgt aatgtcacgc cgctttccgc catttgcttg cttaatgcgg
    71641 gcaggaagct atcggcgatg tttttattta ccagcaacgt ttctaccgta ttacatgtgc
    71701 tcggacgctg agttttcgcg ttgacaatta ctttcagggc ttcagcgatc tctgcacttt
    71761 catcaacgta aatatggcat acgcctatac cgcctgtgat caccgggatt gtcgactgtt
    71821 cgcggcacag cttatgcagg ccagcgccac cacgcgggat cagcatgtcg atgtatttat
    71881 ccatacgcag catttcactg accagcgcgc ggtcaggatt atcaatcgcc tgtactgcgc
    71941 ctgccggtaa atcgcaggat ttcagggcgt cctgaatcac cgccaccgtt gcagcgttag
    72001 tgcgacaggt ttctttgcca ccacgcagga tcactgcgtt accggttttc aggcacagcg
    72061 aagcgacatc aaccgtcacg ttcgggcgag cttcataaat cacgccaatc acccccagcg
    72121 gtacgcgacg acgctccaga cgcagaccgc tgtccagcac accaccatca atcacctgcc
    72181 ccaccggatc ggcgaggttg cacacctggc gcacatcatc ggcaatgcct ttcagccgtg
    72241 cgggcgtcag tgccagacgg tcaagcatcg cttcgctaag gccattggca cgcgcgtcag
    72301 caacatcctg ggcgttagcg ttgaggatga tttcgctttg tgcttccagt tcatcggcga
    72361 ttttttccag cacgcgattt ttttcgcgac tggagagttg cgctaattta tacgaggctt
    72421 gcttcgcggc aatgcccatt tgttccagca tcggccagct ccttaacggg taatcatgtc
    72481 atcacggtga acggcaaccg ggccgtattc atatcccagt attgcatcta tttcttgcga
    72541 gtggtgtccg gcaatacggc gtaatgcatc gctgttgtaa cgactgacgc cgtgggcgat
    72601 atcgcgacct tcgaggttac aaatgcggat cacttcacca cgcgagaagt tgccagtcac
    72661 gcttttaata cctttcggca acagggagct gccgcgttca agaatggcgg cggttgcccc
    72721 ttcatctacc gtgatttcac ccgcaggtgg tgcaccgaaa atccagcgtt tacggttttc
    72781 aagcggggtc gcctgggcat ggaacagcgt accgacggaa atgccttcca tcacgtcacc
    72841 aataacgccc ggcttgctgc ccgcggcaat aatggtgtcg atacccgcac ggcaagccac
    72901 gtccgcggcc tgcaatttgg tactcatgcc gccagttccg aggcctgaaa cgctgtcgcc
    72961 agcaatcgcg cgcagtgcgt catcaatgcc gtaaacatct ttaatcagtt ctgcctgcgg
    73021 attgctgcgc gggtcagcgg tatacaaacc tttttgatcg gtcagcagca acagtttatc
    73081 ggcacccgcc agaatcgccg ccagcgcaga aaggttatcg ttatcgccga ccttaatctc
    73141 tgcagtcgcg acagcatcgt tctcattgat taccggaacg atattgttat cgagcaacgc
    73201 acgcagggtg tcgcgggcgt tcaggaagcg ttcacggtct tccatatcag cacgggtcag
    73261 cagcatttgc ccgacgtgaa tgccataaat agaaaacagc tgttcccaca gttgaatcag
    73321 tcgactctgc cctaccgccg ccagcagttg tttcgaggcg atagtcgctg gtagttctgg
    73381 gtaacccagg tgctcacgtc cggcggcaat cgcgcccgac gtcacaataa caatccgatg
    73441 cccggcggca tgtaactgcg cgcactggcg aacgagttca acaatatggg cacggttcag
    73501 gcggcgcgat ccgcctgtta gcacactggt gccgagtttt accaccagcg tctggctgtc
    73561 actcatgatt ctctgccatt caattttagg aaaaatgata tcaaacgaac gttttagcag
    73621 gactgtcgtc ggttgccaac catctgcgag caaagcatgg tgttttgttg cgcgggatca
    73681 gcaagcgtag cggcagttgt ttacgctttt attacagatt taataaatta ccacatttta
    73741 agaatattat taatctgtaa tatatcttta acaatctcag gttaaaaact ttcctgtttt
    73801 caacgggact ctcccgctga atattcgcgc gttaattaaa atcaggaatg aaaatgaaaa
    73861 agagcactct ggcattagtg gtgatgggca ttgtggcatc tgcatccgta caggccgcag
    73921 aaatatataa caaagacggt aataaactgg atgtctatgg caaagttaaa gccatgcatt
    73981 atatgagtga taacgacagt aaagatggcg accagagtta tatccgtttt ggttttaaag
    74041 gcgaaacaca aattaacgat caactgacgg gttatggtcg gtgggaagcg gagtttgccg
    74101 gtaataaagc ggagagtgat actgcacagc aaaaaacgcg tctcgctttt gccgggttga
    74161 aatataaaga tttgggttct ttcgattatg gtcgtaacct gggcgcgctg tatgacgtgg
    74221 aagcctggac cgatatgttc ccggaatttg gtggcgactc ctcggcgcag accgacaact
    74281 ttatgaccaa acgcgccagc ggtctggcta cttaccgtaa caccgacttc ttcggcgtta
    74341 tcgatggcct gaacttaacc ctgcaatatc aagggaaaaa cgaaaaccgc gacgttaaaa
    74401 agcaaaacgg cgatggcttc ggtacgtcat tgacatatga ctttggtggc agcgatttcg
    74461 ccatcagtgg ggcctatacc aactccgatc gcactaacga gcagaacctg caaagccgtg
    74521 gcacaggcaa gcgtgcagaa gcatgggcaa caggtctgaa atacgatgcc aataatattt
    74581 atctggcaac tttctattct gaaacacgca aaatgacgcc aataactggc ggctttgcca
    74641 ataagacaca gaactttgaa gcggtcgctc aataccagtt tgactttggt ctgcgtccat
    74701 ccctgggtta tgtcttatcg aaagggaaag atattgaagg tatcggtgat gaagatctgg
    74761 tcaattatat cgacgtcggt gctacatatt atttcaacaa aaatatgtca gcgtttgttg
    74821 attataaaat caaccaactg gatagcgata ataagctgaa catcaataat gatgatattg
    74881 tcgcggttgg catgacctat cagttttaat ggatattgcc ggatgtgatg catccggcac
    74941 atttcactca cgccgttaac ttcaccggtt catcacgaaa atcatccgcc ggttccagct
    75001 tcagattcag cgtcgccagc agctcacgca gcttctcgtg aaattcacgc agggtgtgct
    75061 ccagtcgttc aaccacttcg gcatctttta tctgaacact cgtccagtcg cctgctttat
    75121 cgaacagacc aaactggtaa ctgtaggtaa aacgggattc ctgcgcttcc agttccatcc
    75181 accagcccca gaactcacgc acttccggtg ccggtttcac gttgacgcat acagccagac
    75241 aatcgaaaaa gaatcgatta tcttcgcact taccttcacg aatatacggg cctagtgcgg
    75301 taaatttttt gatcaatctg ctcttcgggt gtccactcgg taacgtcatt gcaatctcct
    75361 gttgtgatgc aactgtttta ccaaattggt aaaatttagc aaatttttaa cacaatcgtt
    75421 tttcaatcca gtcggtgatt tcctgaagac ctttgtcaaa attccgatac accgggttaa
    75481 atgggatctc taacaattta ccgtcagcag atgatgaggt gattaagcgt gagtcctctt
    75541 ccgggctgaa cggatcgttc ttccagtagc ctgataacat tggcgttggg cagcgacgtc
    75601 ccagcaatcc ttgcactttt aatgaatagc gattcagctc cacgcgcaac gcttcgtcgg
    75661 aagcatcatg catccccaaa cggctcgcca gaacgtcaag atacatttcc ggcacctgtt
    75721 gctggcactt aaaatcactc aacagggtat gaactaccgg accaagacag gcaaccgctt
    75781 tcagacgcgg cgattcaagg tatgccagac gcacggcaac gttagcgccg aaacggaaac
    75841 caaaggccgc gacgcgagtg tgatctaccc acggtacgtt aggcagcgcc tttaagacgt
    75901 gctgatgcaa caggctggag tcctgggtga gcttccattt tgaagaaaag cccaccgacg
    75961 gcatatcaat agtcagcatc gcaatgccgc gcggcgcaaa ataacgttca tacaggctgt
    76021 aatagtccgt ctgcatcgca tccagaccac cacacattaa tactgtcggg aacgggccat
    76081 cgcctttcgg catatgcaaa aagccggtga tgggcgcacc gccgggtacg gtaaactcca
    76141 tctgccgcat ggtgcccggc agacgctgag cggcctcttc ataggcgcgg tttgacaaag
    76201 cctgcgcttg ctcggccagg tcatctcctt tcagatgagg ataggcggca atgttgtaca
    76261 acgtagccgc atgcagccag tgacgaccac tgagttgcgg atcttcttcc gcacaggctt
    76321 tttgttgcca caccatcgcc tgggtggccc actcataaat ccagttgcca ccacggtagc
    76381 caatcaccgt atcgtataaa tcatcgtcgg tacgttcggc atcgctcatc acaatacgtg
    76441 cctggacgtc gaggatttca cgtgggtcaa tgccgcgcca gatccacatc agacggttaa
    76501 tcatgcgata ccagtgaggg atggttttac catcaagggc cgactgcaca ggcggttgtg
    76561 cgccgtgatt aaagcggcgg actagcgtcg aggtttcagg atgtttaaag cggggtttga
    76621 acagggtttc gctcaggttt gcctgtgtca tggatgcagc ctccagaata cttactggaa
    76681 actattgtaa cccgcctgaa gttaaaaaga acaacgcccg gcagtgccag gcgttgaaaa
    76741 gattagcgac cggagattgg cgggacgaat acgacgccca tatcccacgg ctgttcgatc
    76801 caggtatctt gcgggatatc aacaacataa tcatcaacca gcgggcgacc agccggtttt
    76861 gcgaagatgg tgacaaagtg cgcttttgga tacatttcac gaatcgcaac cgcagtacca
    76921 ccggtatcca ccaggtcatc aataacgatg aagccttcgc catcgccttc tgcgcgtttc
    76981 agcactttaa gctcgcgctg gttgtcgtga tcgtagctgg aaatacaaac ggtatcgaca
    77041 tgacgaatac ccagttcacg cgccagtaac gcacccggta ccagaccgcc acggcttacg
    77101 gcaataatgc ctttccattg ttcagaaggc atcagtcggc ttgcgagttt acgtgcatgg
    77161 atctgcaaca tgtcccaggt gacgatgtat ttttcgctca tgtgaagtgt cccagcctgt
    77221 ttatctacgg cttaaaaagt gttcgagggg aaaataggtt gcgcgagatt atagagatct
    77281 ggcgcactaa aaaccagtat ttcacatgag tccgcgtctt tttacgcact gcctctccct
    77341 gacgcgggat aaagtggtat tctcaaacat atctcgcaag cctgtcttgt gttgacaaca
    77401 ttttctgcta accctgtgac ctgcaatact gttttgcggg tgatcgacaa ggagacttaa
    77461 cgtgtctgaa ctgtctcaat tatctccaca gccgctgtgg gatatttttg ccaaaatctg
    77521 ttctattcct cacccgtcct atcatgaaga gcaactcgct gaatacattg ttggttgggc
    77581 aaaagagaaa ggtttccatg tcgaacgcga tcaggtaggt aatatcctga ttcgtaaacc
    77641 tgctaccgca ggtatggaaa atcgtaaacc agtcgtcttg caggcccacc tcgatatggt
    77701 gccgcagaaa aataacgaca ccgtgcatga cttcactaaa gatcctatcc agccttatat
    77761 tgatggcgaa tgggttaaag cgcgcggcac cacgctgggt gcggataacg gcattggtat
    77821 ggcgtctgcg ctggctgttc tggctgacga aaacgtggtt cacggcccgc tggaagtgct
    77881 gctgaccatg accgaagaag ccggtatgga tggtgctttt ggcttacaga gcaactggtt
    77941 gcaggctgat attctgatta acaccgactc cgaagaggaa ggtgaaatct acatgggttg
    78001 cgcggggggt atcgacttca cctccaacct gcatttagat cgtgaagcgg ttccagctgg
    78061 ttttgaaacc ttcaagttaa ccttaaaagg cctgaaaggc ggtcactccg gcggtgaaat
    78121 ccacgttggc ctgggtaatg ccaacaaact gctggtgcgc ttcctggcgg gtcatgcaga
    78181 agagctggac ctgcgcctta tcgatttcaa cggtggcaca ctgcgtaacg ccatcccgcg
    78241 tgaagccttt gcgaccattg ctgtcgcagc tgataaagtc gacgccctga aatctctggt
    78301 gaatacctat caggagatcc tgaaaaacga gctggcagag aaagagaaaa atctggcatt
    78361 gctgctggac tctgttgcga acgataaagc tgccctgatt gcgaaatctc gcgatacctt
    78421 tattcgtctg ctgaacgcca ccccgaacgg tgtgatccgc aattccgatg tggcaaaagg
    78481 tgtggtggaa acctccctga acgtcggtgt ggtgaccatg actgacaata acgtagaaat
    78541 tcactgcctg atccgttctc tgatcgacag cggtaaagac tacgtggtga gcatgctgga
    78601 ttcgctgggt aaactggctg gcgcgaaaac cgaagcgaaa ggcgcatatc ctggctggca
    78661 gccggacgct aattctccgg tgatgcatct ggtacgtgaa acttatcagc gtctgttcaa
    78721 caagacgccg aacatccaga ttatccacgc gggcctggaa tgtggtctgt tcaaaaaacc
    78781 gtatccggaa atggacatgg tttctatcgg gccaacaatc accggtccac actctccgga
    78841 tgagcaagtt cacatcgaaa gcgtaggtca ttactggaca ctgctgactg agctgctgaa
    78901 agaaattccg gcgaagtaat tatttgattt gctgccggat ggcgtttaat cgccttccgg
    78961 cagtttcatc cttcattatc cttcgataaa agccatccct gtaaatgtcc gtctcgggtt
    79021 gccacgttca atctggtgat ggaacattcg ccgccgcgat ttcagcaccg cgctattttc
    79081 ctgttgctgt tgttccagcg tccaggcaat cagcaatcgc gccagccgct tgttagcatg
    79141 cccagaaaca agtttttgcg cccatgatga ggccgatacg gactcggaca aatccactga
    79201 atagtgccgc accaactttc gcttaatgcc catgcgttat cgccatccag agaaatcagc
    79261 gccgaacgca gcgtgtcaga gtagcggccc gtttctgttt ccagcaccgt taccgcgacg
    79321 tcttgccggg taaaaacgac caacagagaa taacgctgcg ccaatcgggt agccgcgtcc
    79381 ggggtgtaaa tctggcatcc ctactacgcg ctgcatgttg ggtaaatttg ccgtggtgtg
    79441 taattgttga atcgctaaac tctcgatcca caggtcatca gatgcgatgg taaataccgc
    79501 atcggataag ggacgaatat atttgcccat gtatcattcc atttatactc gtcatacttc
    79561 aagttgcatg tactgcgact cgaattattt agagtatata aatataaaaa tcaggaaatg
    79621 gcagacgaat ccagggcaag aaaaaagaat taatcctgga aaggtctgca aagagtcatt
    79681 gataaaggat ttgtcatgat ttacctcctt gcagtttgtg gaataagaaa acgcgcacat
    79741 accaggcggg cgttattttc attgcaagct ggatttaatg ttgcggttta tatcgcatag
    79801 aaaaattagt aaaccacgct cccctgcatt caacgcgctg ctgcttaact gtctgaaaac
    79861 cataacgttc aaaaaagggt tttgcggtta tgctggcgtc cactgtaagt tcggattcag
    79921 acttaatcaa aggttttaac aaagcgctgg caaccccacg gcgggtgtat tcagggtcaa
    79981 caaataacat atcgatatag tgttcaacgc aggtaataaa gccgaccagt tttgcattaa
    80041 tgactgcaac ccgcacttgt gatttcgcga gtttctcctt ccagcgagat tcgtcaatct
    80101 gcgcccaggc ggcaatttgt tgtggtgaat aatgctgact ggcggtcatc gtaaccgctc
    80161 taatgaaaat agcgcatagt tgctgaaaat cgccaggctg ataatctctt atttgtatgt
    80221 tattcataat ataaattcaa aaacgcatgc gaaacgcttc tgccattttt ccaattttat
    80281 gcatttggtt gttatctcga gccagtttat gaggttcagg cttcagaatg gcaatgagca
    80341 accatgcttg ctcatcaaac gccccctgac aatataccaa atgcgcttcg tcattcgttc
    80401 tgctgaattg gcgcaactgt ggcggaaatg gattattcac atttgccaga tgaatatgag
    80461 caactcgctc aaatttgatt aatggccagg taaacgagtc gtcgtagagt gcatcgcgac
    80521 caaatatatc tggcaaaata ccgtcacgct tataggaaat aaaatccgcc gttaacgcat
    80581 caagttcctc tgctgtaagt tgcaggcgaa taagttttgt tttgaatacc cgcatcctta
    80641 ttccttaaag tcattgaaat catcatccgt catatcatta agatattgtt cagcgcggcg
    80701 tgtaatttcc tctaatcttg cagcactcgg acggaagcgc gcctttatgg gctttttctc
    80761 ctcttgttca gcaagcatgt ccattaacgc ttcgaatgcg ctggcactta agagatatcc
    80821 tgcggggcga ttattagaaa gaaccgcaac cggttgatca ataaagtatt tagctgggtt
    80881 tttacgtaac tcagtgatat tgaccgattt ttcagcgaga attcgatgca tacagtgatc
    80941 ccctcataat aatgcacacc agaatagaca taatagtata catcataatc ccagcaccag
    81001 ttgtctttcc atttgagggt caagcaacgt cacatgcagc cccaccagtc gcacaccgcg
    81061 cccgccgcgg cgttcatccc aggttttacg cgcggtggcg attagatcag ctttattcag
    81121 ccgcggccag acgtgctcct gagtagtttg ctgaaaatcg tcgaacttta atttcacccc
    81181 ctggcgagca atcagtaaat caggttttac ttttgccaga cgacgttcaa gttccggata
    81241 cagccgctcg ataatcgctt cacattcaga ccagtgatga atatcttccg ccatcgtgcg
    81301 ttccacgccg acagattttc gcaaccgttc gctgttaacg tcgcgctcgt caatcccctg
    81361 actacgctcc cacaaaatgc ggccaaattt gccaaaacgt ttgagcagag tcaccagatc
    81421 acacttttgc acatcaccgc aggtccgcag ccccatcgct tccagttttg ccgccgagac
    81481 tttgccgacg ccggggattt ttgccagcgg taaggtttgt aaaaatgtcg gaacttctgc
    81541 cggcgtaatc acaaactggc cgttgggttt attcatgtcg gaggcgattt tggcgagaaa
    81601 ctttactggt gccacgcccg cagacgccgt cagttgcagc tcgttgaaga ttgtctggcg
    81661 gatttcctgg gcgatgaggg tcgcagaacc gtggcaatgg acgctatcag tgacatcgag
    81721 ataggcttca tccagtgaca acggttcaat acgcgaggtg tagcgcgaga agatttcacg
    81781 gatatgattt gaggcttctt tgtaggcgtc aaagcgcccc ggaagcaagg tgagatgtgg
    81841 gcataatttg agcgccatcc ctgtcggcat agcgctacgt acgccaaatt tacgcgcggg
    81901 ataattggcg gtgctgatca ccccccgacg ttcgcggctg ccgccaatag caatagggat
    81961 atcgcgcagg gcgggattgt cgcgcatctc cactgcggcg aaaaagcagt ccatatccac
    82021 atgaatgatt ttacgcattg ctcacctctc aacactggta aagtatacag tgatttcagg
    82081 gtttgagaaa tgcgtaaaga ttcagcatct agcgcgttcg ctgcgaaagc acctgctgct
    82141 tatccagctt ttgcaccagc ggctgcacca ctttatcccc atgctgacca aagtattgat
    82201 acagcgtatc ggacgcactt ttggtcagca ccataatctc aatgcgtcgg ttgcccgcgc
    82261 tttgcggatt tttggcatcc agcagcatct ggtccgccat tgcgcttacc tgcatcactt
    82321 tatcttccgg cattccagcc tcttccagca cccgacgggc cgaaagcgcg cgatcgcccg
    82381 aaaggttcca gttgttgtag atattgtttt tgtaggccat cgcatcggta tgccctgtaa
    82441 taataatttt gttatcgagc gagtcgaaca ctggcgcgag ctccaccagc aaagttttaa
    82501 agaacggcat aatctgtgcg ctgccgcgtt caaacatatt gcggttctgg tcatctttaa
    82561 tcagcacgcg caatccctgg ggaacaatct ccatctccag attcgcttcc atatgtgcat
    82621 cacgggcaat ggtgttgata ctggtcgcca gctcgccaag ctcagtagcg gatttcttct
    82681 tcagcagcgc cgttttctca ttgacgtcac gcgctttttt ctccgtctct tccggcaccg
    82741 ccacggtggc aggttttggc ggatgagacg ggctgatttt atttaacggc gacaacccgc
    82801 cgccattgaa aatcgactgc ccgtgcagcg cggcgataat gctttcacgc tcggatttac
    82861 tgacgctgtt gacaatcaac tttatcgtcg tcaccaaagg ggccgagcgt atttccgagg
    82921 tttctgcccg ctttaccctg gacgcgatgc ccggcaaaca gatggcgatt gacgccgatc
    82981 ttaacgccgg gttgatcaac caggcgcagg cgcaaacccg gcgtaaagat gttgccagcg
    83041 aggccgattt ctacggcgca atggacgggg catcgaagtt tgtgcgcggg gatgccatcg
    83101 ccgggatgat gattctggcg atcaacctga tcggcggcgt ctgtatcggg atcttcaaat
    83161 acaacctgag cgccgatgcc gccttccagc aatatgtgct gatgaccatc ggcgacggcc
    83221 tggtggcgca gatcccgtcc ctgctgcttt ccaccgcggc ggcgattatc gtcacccgcg
    83281 tcagcgacaa cggcgatatt gcccatgacg tgcgtaacca gttgctggca agcccgtcgg
    83341 tgctctacac cgctaccggg atcatgtttg tgctggcggt ggtgccgggg atgccgcatc
    83401 tgccattttt gctgttcagc gccctgcttg gctttaccgg ctggcggatg agcaaacgcc
    83461 cgcaggcggc ggaggcggaa gagaaaagcc tcgaaacgct gacccgcacc atcaccgaaa
    83521 ccagcgagca acaggtcagt tgggaaacta ttccgctgat cgaacctatc agcttaagcc
    83581 tcggctacaa gctggttgcg ctggtcgata aagcgcaggg taacccactc acccagcgta
    83641 ttcgcggcgt gcggcaagtg atatccgacg gcaacggcgt gctgctgccg gagatccgta
    83701 ttcgggaaaa cttccgcctc aagcccagtc agtacgccat tttcatcaac ggcattaagg
    83761 ccgatgaagc ggatattccg gcggataaac tgatggcgct gccctccagc gaaacctacg
    83821 gcgagataga cggcgtgctg gggaacgacc cggcgtacgg gatgccggtg acctggatcc
    83881 agcccgcgca gaaagcgaaa gcgctgaata tggggtatca ggtgatcgac agcgccagcg
    83941 tgattgccac gcatgtgaac aagattgtgc gcagctatat tcctgatcta tttaactatg
    84001 atgacatcac gcagttgcat aaccgtttgt cgtcgatggc accgcgactg gcggaggatt
    84061 taagcgcggc gctcaattac agccagttgc tgaaagtgta ccgggcgctg ctgaccgaag
    84121 gcgtttccct gcgcgatatc gtcaccatcg ccaccgtgct ggttgccagt agcgcggtga
    84181 ccaaagatca tattctgctg gcggcagatg tgcgcctggc gctgcggcgc agcattaccc
    84241 atccgttcgt tcgcaagcag gagctgacgg tgtatacgct gaataatgag ctggaaaatc
    84301 tgctgaccaa cgtagtgaat caagcgcaac aggccggcaa agtgatgctc gacagtgtgc
    84361 cggtggaccc gaatatgctt aaccagttcc agagcacgat gccgcaggta aaagagcaga
    84421 tgaaagcggc ggggaaagac ccggtgctgc tggtgccgcc gcaactgcgt ccattgctgg
    84481 cgcgctatgc aaggttgttt gcgccggggc tgcatgtgct gtcgtataac gaagtgccgg
    84541 atgagctgga gttgaagatt atgggggcgc tgagttgatg ccttttaaga aacacaatac
    84601 gatgacatca atttatgacg tcagggattg ataagatgat gaaaacgcgt ttattaatat
    84661 ctcaagcatc gaacattatc accattaaag gtcaaccagc cgcagtgaca tttgaagccg
    84721 atatcaacgc atttcgcggc aaatttctca atgtaaatgg ttattgcgac tttgtagcga
    84781 caagcattga ggcgttatac cgggaaggtg aaagtgcgct ggatgagtgg atggcggatt
    84841 gtgaagagga cggcattgcc ccttttcgtg aagaagagca aaaacgcctg acgcttcgtg
    84901 ttcctcatca cattgattcg cggctgacca tcgtcgccag acaacattcc atttcaaaaa
    84961 accagctcat tgttgatgtc ttagaacgtg agttttcagc ccagatgtaa acaggtcgga
    85021 tgccgcgtgg catccgacct tttgccagtt cgtcaatatt acccatttac aaaatcgtct
    85081 cttccgtcaa aatccggcgc gcgcctaaaa aatggtcctg ccagaaaggt tcagctaacc
    85141 gacttacccg tatggtttcc ccggtgcgcg gcgactcaat aaactgtcca tcgcccagat
    85201 acacgcccat atggtcggca atctcgcggc tgtggatatg aaaaaacagc aaatctcccc
    85261 ggcgcaggtc gttattcgcc acaatggttg cccggcgata gtgatacatc tcattagccg
    85321 tgcgtgggag cttcgcctcg aggatcttgt tgtaggcata aaaaaccaac ccgctacagt
    85381 cgaagccttg atcgggccgc gtgccgcccc agacgtaagg cttgccaagc tgctgctcca
    85441 ggcggtgaat cgccacttcg gtgatgttgt gtagatggtc gctgcttaag aaatggttgt
    85501 tctccgccag cgcctgccag cgtctgtcac tcgccttgag cggcgcagga aaccattctg
    85561 gatgctgttt tatctgttcg cggttgtgct ggcgcagcgc ctttctgctt tcggcattgc
    85621 cttccacaat gtagctggcc tgctttttca gatgggtttg gtactgtttt aataaacgcg
    85681 cccggttttg catccgctgg cgggcggcgt agctgaaaga gatatgtgat gtggtggcgc
    85741 tggcgagtaa agggagcaaa acgcataacg aaaacacgcc tggatgcaga aatctgccgt
    85801 gaaagaacga agacatgaag gacataaact agctgaatga aggagagaat tttaaaaggt
    85861 tgggggagta aacgggatta ttaattgctt atttgcatat tagttacgag gtttcataat
    85921 cttaggaaat aagtgataca tgcgcttttc ggactaatga aaactgcccc tgtgctaaat
    85981 ttgtaaaccc tgcgccgaca ggcttgcgta aaactctttt ggtgataatg tgagccttcc
    86041 aaatccagac tattccgatt tgggatccat tttgccgtcc tggcgtgtgt aaccgtttta
    86101 tcaaggaata agcagtatgc gtaaaatcgc attaattctt gcgatgttgt tgatcccgtg
    86161 cgtttcgttt gccggtttgc tgggcagcag tagctcaacc acgccagtga gcaaagagta
    86221 taagcagcag ttgatgggat cccctgtcta catccagatc ttcaaggaag aacgtacgct
    86281 cgatctctac gtcaaaatgg gcgagcaata tcagctgctc gacagttata aaatctgtaa
    86341 atattccggc ggcttagggc caaaacagcg tcagggcgat ttcaaaagcc cggaagggtt
    86401 ttatagcgtc cagcgtaatc agttaaaacc agacagccgt tactacaaag caattaatat
    86461 tggattcccc aatgcctatg accgtgcgca tggttatgaa gggaaatacc tgatgatcca
    86521 cggcgattgt gtttccatcg gctgctacgc gatgaccaat cagggtattg atgagatctt
    86581 ccagttcgtt actggtgcgc tggtttttgg tcagccgagc gtgcaagtga gtatttaccc
    86641 gttccgcatg accgacgcca atatgaagcg ccataaatat tccaacttta aggacttctg
    86701 ggagcagctg aagccaggtt acgactactt tgagcaaacc cgtaagccac cgaccgtttc
    86761 tgtggtcaat ggtcgttacg tggtcagcaa gccgttaagc cacgaagttg tgcagccaca
    86821 gctggcatca aactacacgc tccccgaggc aaaataagcg ccattcgcct ggcataatct
    86881 tttgccaggt ttcattgccc gtcagcggct gtgttgcaat caccgtgacc acatcattcg
    86941 gtgtggtctg cgagctgaag tcgatttcca catcctgatc cagcaacgtt gccacgccaa
    87001 acggcgcacg acgggtgatc cagtgtaaat tagtcgagca atacgccatt acatagcgcc
    87061 cgtcagaaag cagcatgttg aaaacgccct tctgccgcag ttcatccgcc agtgaggcga
    87121 tatatttaaa cactgccgcc atgttgcccg gtgtgcgcgg gtaacgctgc gttaatttat
    87181 gcaggagcca gcagaaggct ttttcgctgt cggtttcacc gaccgggcgg aagttgccgg
    87241 tttccagtga tttatagccc gtcagttgcc cattatgggc ataagtccag ttacgccccc
    87301 ataactcgcg ggtaaacggg tgagtatttt ccagcgccac ctcgccccga ttcgcctggc
    87361 gaatatgagc caccaccgaa caggatttta tcgggtagtc ctggacaagt ttggcgatgg
    87421 gggaattaaa gctgggttgc ggatctttaa atgtgcgaca gcctttacct tcgtaaaagg
    87481 taatgcccca gccatcttta tgtggcccgg ttccaccacc acgctgtaca agtccggtga
    87541 aactaaagca gatatcggta gggacgttgg cgctcatccc gagcaattcg cacatgggta
    87601 acctccacaa cagcgggagc gcatggctcc cggcgaagat tgattaaaaa gtacaaaggt
    87661 gcaggctgtt accctcaccc cggccctctc cctgaaaggg agaaaagacc ttacttaacc
    87721 atctcttttt caattaactg gatcaggata tggatcactt taatgtgaat ctcctgaatg
    87781 cggtcggcat aaccaaagtg cggtacgcga atttcgatat ccgccgtgcc agccattttg
    87841 ccgccgtctt taccggtcag ggtgatcact ttcatcccct tctcacgcgc cgccgcgatc
    87901 gctttgatca cgttcgcaga gttaccggaa gtggagatcc ccagcagtac atcgccttcg
    87961 cgacctaccg cttcaacgta acgggagaaa atatcattga aaccaaaatc gttaccgacg
    88021 caggaaatat gactaacgtc agaaatggca atcgccgggt agcccggacg gttttcacgg
    88081 tagcgaccgg tcaactcttc ggcaaagtgc atagcgtcgc aatgggagcc gccgttgcca
    88141 caggaaagca ccttgccacc ggctttaaag ctgtctgcta acaggaccgc cgcgcgctga
    88201 atggcgtgaa tattggcgtc atcttttaaa aagttagcca gcgtttccgc cgcttcgttc
    88261 agttcgttac gaataagatc ctggtacatg aggatatcct tcagcataaa tgtaatagac
    88321 aaaatgcagt gtaccggata ccgccaaaag cgagaagtac gggcaggtgc tatgaccagg
    88381 actttttgac ctgaagtgcg gataaaaaca gcaacaatgt gagctttgtt gtaattatat
    88441 tgtaaacaca ttgctaaatg tttttacatc cactacaacc atatcatcac aagtggtcag
    88501 acctcctaca agtaaggggc ttttcgttat gatgattttg agtattctcg ctacggttgt
    88561 cctgctcggc gcgttgttct atcaccgcgt gagcttattt atcagcagtc tgattttgct
    88621 cgcctggaca gccgcactcg gcgttgctgg tctgtggtcg gcgtgggtac tggtgcctct
    88681 ggccattatc ctcgtgccat ttaactttgc gcctatgcgt aagtcgatga tttccgcgcc
    88741 ggtatttcgc ggtttccgta aggtgatgcc gccgatgtcg cgcactgaga aagaagcgat
    88801 tgatgcgggc accacctggt gggaaggcga tttgttccag ggcaagccgg actggaaaaa
    88861 gctgcataac tatccgcagc cgcgcctgac cgcagaagag caagcgtttc tcgacggccc
    88921 ggtagaagaa gcctgccgga tggcgaatga tttccagatt actcatgagc tggcggatct
    88981 gccgccggag ttgtgggcgt accttaaaga gcatcgtttc ttcgcgatga tcatcaaaaa
    89041 agagtacggc gggctggagt tctcggctta tgcccagtct cgcgtgctgc aaaaactctc
    89101 cggcgtgagc gggatcctgg cgattaccgt cggcgtgcca aactcattag gtccgggcga
    89161 gctgctgcaa cattacggca ctgacgagca gaaagatcac tatctgccgc gtctggcgcg
    89221 tggtcaggaa atcccttgct ttgcactgac cagcccggaa gcgggttccg atgcaggcgc
    89281 tatcccggat accgggattg tctgcatggg cgaatggcag ggccagcagg tgctgggaat
    89341 gcgtctgacc tggaacaaac gctacattac gctcgcaccg attgcgaccg tgcttggtct
    89401 ggcgtttaaa ctctccgacc cggaaaaact gctgggtggc gcagaagatt taggtattac
    89461 ctgtgcgctg atcccgacca ccacgccggg cgtggaaatt ggtcgtcgcc acttcccgct
    89521 aaacgtaccg ttccagaacg gaccgacgcg cggtaaagat gtcttcgtac cgatcgatta
    89581 cattatcggc ggaccaaaaa tggccgggca aggctggcgg atgctggtgg agtgcctctc
    89641 ggtaggccgc ggcatcaccc tgccttccaa ctcaaccggc ggcgtgaaat cggtagcgct
    89701 ggcaacgggt gcgtatgcgc acattcgccg tcagttcaaa atctctatcg gtaagatgga
    89761 agggattgaa gagccgctgg cgcgtattgc cgggaatgcc tacgtgatgg atgctgcggc
    89821 atcgctgatt acctacggca ttatgctcgg tgaaaaaccc gccgtgttgt cggctatcgt
    89881 taagtatcac tgtacccacc gggggcagca atcgattatt gatgcgatgg atatcactgg
    89941 tggcaaaggc attatgctcg ggcagagcaa cttcctggcg cgtgcttacc agggcgcacc
    90001 gattgccata accgttgaag gggctaacat tctgacccgc agcatgatga tcttcggaca
    90061 aggagcgatt cgttgccatc cgtacgtgct ggaagagatg gaagcggcga agaacaatga
    90121 cgtaaatgcg ttcgataaac tgctgttcaa acatatcggt cacgtcggta gcaacaaagt
    90181 tcgcagcttc tggctgggcc tgacgcgcgg tttaaccagc agcacgccaa ccggcgatgc
    90241 cactaaacgc tactatcaac acctgaaccg cctgagcgcc aacctcgccc tgctttctga
    90301 tgtctcgatg gctgtgctgg gcggcagcct gaaacgtcgc gagcgtatct cggcacgtct
    90361 gggggatatt ttaagccagc tttacctcgc ctctgccgtt ctgaagcgtt atgacgacga
    90421 aggccgtaat gaagccgatc tgccgctggt gcactggggc gtacaggatg cgctgtatca
    90481 ggctgaacag gcgatggacg atttactgca aaacttcccg aaccgcgtgg ttgccgggct
    90541 gctgaatgta gtgatcttcc cgaccggacg tcattatctg gcaccgtctg acaagctgga
    90601 tcataaagtg gcgaagattt tacaagtgcc gaacgccact cgctcacgta ttggtcgcgg
    90661 tcagtacctg acgccgagcg agcataatcc ggttggcttg ctggaagagg cgctggtgga
    90721 tgtgattgcc gccgacccga ttcatcagcg gatctgtaaa gagctgggta aaaacctgcc
    90781 atttacccgt ctggatgaac tggcgcacaa cgcgctggcg aaggggctga ttgataaaga
    90841 tgaagccgct attctggtga aagctgaaga aagccgtctg cgcagtatta atgttgatga
    90901 ctttgatccg gaagagctgg cgacgaagcc ggtaaagttg ccggagaaag tgcggaaagt
    90961 tgaag
//
LOCUS       DAPOZO010000004        82597 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPOZO010000004 DAPOZO010000000
VERSION     DAPOZO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 82597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.004"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            6..902
                     /locus_tag="ROI21_000290"
     CDS             6..902
                     /locus_tag="ROI21_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDX5109266.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            983..1504
                     /gene="sodC"
                     /locus_tag="ROI21_000291"
     CDS             983..1504
                     /gene="sodC"
                     /locus_tag="ROI21_000291"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDX5109267.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(1505..3517)
                     /locus_tag="ROI21_000292"
     CDS             complement(1505..3517)
                     /locus_tag="ROI21_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDX5109268.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPCATREIL
                     EQLLTALASSQTDIYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPWTSGLARHTDNAEAMWSGLRTFCTLMMIGTWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICYSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3517..4374)
                     /locus_tag="ROI21_000293"
     CDS             complement(3517..4374)
                     /locus_tag="ROI21_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDX5109269.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLTKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTKIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYRDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(4377..4613)
                     /locus_tag="ROI21_000294"
     CDS             complement(4377..4613)
                     /locus_tag="ROI21_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDX5109270.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            4814..5248
                     /gene="slyA"
                     /locus_tag="ROI21_000295"
     CDS             4814..5248
                     /gene="slyA"
                     /locus_tag="ROI21_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDX5109271.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(5299..5766)
                     /gene="slyB"
                     /locus_tag="ROI21_000296"
     CDS             complement(5299..5766)
                     /gene="slyB"
                     /locus_tag="ROI21_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDX5109272.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            6040..7149
                     /gene="anmK"
                     /locus_tag="ROI21_000297"
     CDS             6040..7149
                     /gene="anmK"
                     /locus_tag="ROI21_000297"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDX5109273.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            7247..7576
                     /gene="mliC"
                     /locus_tag="ROI21_000298"
     CDS             7247..7576
                     /gene="mliC"
                     /locus_tag="ROI21_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDX5109274.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            7635..8291
                     /gene="pdxH"
                     /locus_tag="ROI21_000299"
     CDS             7635..8291
                     /gene="pdxH"
                     /locus_tag="ROI21_000299"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDX5109275.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPAEPLTLFERWLSQACD
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            8420..9694
                     /gene="tyrS"
                     /locus_tag="ROI21_000300"
     CDS             8420..9694
                     /gene="tyrS"
                     /locus_tag="ROI21_000300"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDX5109276.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            9756..10616
                     /gene="pdxY"
                     /locus_tag="ROI21_000301"
     CDS             9756..10616
                     /gene="pdxY"
                     /locus_tag="ROI21_000301"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDX5109277.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(10660..11265)
                     /gene="gstA"
                     /locus_tag="ROI21_000302"
     CDS             complement(10660..11265)
                     /gene="gstA"
                     /locus_tag="ROI21_000302"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDX5109278.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNDALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(11370..12872)
                     /gene="dtpA"
                     /locus_tag="ROI21_000303"
     CDS             complement(11370..12872)
                     /gene="dtpA"
                     /locus_tag="ROI21_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDX5109279.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(13483..14118)
                     /gene="nth"
                     /locus_tag="ROI21_000304"
     CDS             complement(13483..14118)
                     /gene="nth"
                     /locus_tag="ROI21_000304"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDX5109280.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(14118..14813)
                     /gene="rsxE"
                     /locus_tag="ROI21_000305"
     CDS             complement(14118..14813)
                     /gene="rsxE"
                     /locus_tag="ROI21_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDX5109281.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(14817..15437)
                     /gene="rsxG"
                     /locus_tag="ROI21_000306"
     CDS             complement(14817..15437)
                     /gene="rsxG"
                     /locus_tag="ROI21_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDX5109282.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPSQLSQLPACGE"
     gene            complement(15441..16499)
                     /gene="rsxD"
                     /locus_tag="ROI21_000307"
     CDS             complement(15441..16499)
                     /gene="rsxD"
                     /locus_tag="ROI21_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDX5109283.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGV
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLASPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(16500..18722)
                     /gene="rsxC"
                     /locus_tag="ROI21_000308"
     CDS             complement(16500..18722)
                     /gene="rsxC"
                     /locus_tag="ROI21_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDX5109284.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWDDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACTDACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIHQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEADIARA
                     KARKLEQQQANAKPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEKVDPRKAAVE
                     AAIARAKARKLEQQKANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(18715..19293)
                     /gene="rsxB"
                     /locus_tag="ROI21_000309"
     CDS             complement(18715..19293)
                     /gene="rsxB"
                     /locus_tag="ROI21_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDX5109285.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(19293..19874)
                     /gene="rsxA"
                     /locus_tag="ROI21_000310"
     CDS             complement(19293..19874)
                     /gene="rsxA"
                     /locus_tag="ROI21_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDX5109286.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(19951..20391)
                     /locus_tag="ROI21_000311"
     CDS             complement(19951..20391)
                     /locus_tag="ROI21_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDX5109287.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(20477..20692)
                     /gene="ydgT"
                     /locus_tag="ROI21_000312"
     CDS             complement(20477..20692)
                     /gene="ydgT"
                     /locus_tag="ROI21_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDX5109288.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(20965..21090)
                     /gene="blr"
                     /locus_tag="ROI21_000313"
     CDS             complement(20965..21090)
                     /gene="blr"
                     /locus_tag="ROI21_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDX5109289.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            21333..22373
                     /locus_tag="ROI21_000314"
     CDS             21333..22373
                     /locus_tag="ROI21_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDX5109290.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWLTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(22428..23429)
                     /gene="add"
                     /locus_tag="ROI21_000315"
     CDS             complement(22428..23429)
                     /gene="add"
                     /locus_tag="ROI21_000315"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDX5109291.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(23533..24705)
                     /gene="malY"
                     /locus_tag="ROI21_000316"
     CDS             complement(23533..24705)
                     /gene="malY"
                     /locus_tag="ROI21_000316"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDX5109292.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSEKGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNSALPELNWQIPQSTYLAWIDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(24715..26307)
                     /gene="malX"
                     /locus_tag="ROI21_000317"
     CDS             complement(24715..26307)
                     /gene="malX"
                     /locus_tag="ROI21_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDX5109293.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            26482..27510
                     /gene="malI"
                     /locus_tag="ROI21_000318"
     CDS             26482..27510
                     /gene="malI"
                     /locus_tag="ROI21_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDX5109294.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKNGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDRPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            27622..28389
                     /gene="hdhA"
                     /locus_tag="ROI21_000319"
     CDS             27622..28389
                     /gene="hdhA"
                     /locus_tag="ROI21_000319"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDX5109295.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            28618..29208
                     /gene="uidR"
                     /locus_tag="ROI21_000320"
     CDS             28618..29208
                     /gene="uidR"
                     /locus_tag="ROI21_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDX5109296.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            29599..31410
                     /gene="uidA"
                     /locus_tag="ROI21_000321"
     CDS             29599..31410
                     /gene="uidA"
                     /locus_tag="ROI21_000321"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDX5109297.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESVLQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDFYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            31407..32780
                     /gene="uidB"
                     /locus_tag="ROI21_000322"
     CDS             31407..32780
                     /gene="uidB"
                     /locus_tag="ROI21_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDX5109298.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIDALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKMQQQ
                     LISDITN"
     gene            32819..34084
                     /gene="uidC"
                     /locus_tag="ROI21_000323"
     CDS             32819..34084
                     /gene="uidC"
                     /locus_tag="ROI21_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDX5109299.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLTDDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(34130..35638)
                     /locus_tag="ROI21_000324"
     CDS             complement(34130..35638)
                     /locus_tag="ROI21_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDX5109300.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPKSNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLQGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(35739..36914)
                     /gene="manA"
                     /locus_tag="ROI21_000325"
     CDS             complement(35739..36914)
                     /gene="manA"
                     /locus_tag="ROI21_000325"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDX5109301.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            37113..38759
                     /gene="fumB"
                     /locus_tag="ROI21_000326"
     CDS             37113..38759
                     /gene="fumB"
                     /locus_tag="ROI21_000326"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDX5109302.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            38902..40305
                     /gene="fumC"
                     /locus_tag="ROI21_000327"
     CDS             38902..40305
                     /gene="fumC"
                     /locus_tag="ROI21_000327"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDX5109303.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEIAIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(40302..41231)
                     /gene="tus"
                     /locus_tag="ROI21_000328"
     CDS             complement(40302..41231)
                     /gene="tus"
                     /locus_tag="ROI21_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDX5109304.1"
                     /translation="MARYDLVDRLNTTFRQMEQELGAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINQLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(41308..42609)
                     /gene="rstB"
                     /locus_tag="ROI21_000329"
     CDS             complement(41308..42609)
                     /gene="rstB"
                     /locus_tag="ROI21_000329"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDX5109305.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALHY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(42613..43332)
                     /gene="rstA"
                     /locus_tag="ROI21_000330"
     CDS             complement(42613..43332)
                     /gene="rstA"
                     /locus_tag="ROI21_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDX5109306.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            43461..43796
                     /locus_tag="ROI21_000331"
     CDS             43461..43796
                     /locus_tag="ROI21_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDX5109307.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(43793..44515)
                     /gene="folM"
                     /locus_tag="ROI21_000332"
     CDS             complement(43793..44515)
                     /gene="folM"
                     /locus_tag="ROI21_000332"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDX5109308.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(44552..45934)
                     /locus_tag="ROI21_000333"
     CDS             complement(44552..45934)
                     /locus_tag="ROI21_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDX5109309.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(46120..47064)
                     /locus_tag="ROI21_000334"
     CDS             complement(46120..47064)
                     /locus_tag="ROI21_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX5109310.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQSFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            47588..49120
                     /gene="pntA"
                     /locus_tag="ROI21_000335"
     CDS             47588..49120
                     /gene="pntA"
                     /locus_tag="ROI21_000335"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDX5109311.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPSRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            49131..50519
                     /gene="pntB"
                     /locus_tag="ROI21_000336"
     CDS             49131..50519
                     /gene="pntB"
                     /locus_tag="ROI21_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDX5109312.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(50544..51578)
                     /gene="tqsA"
                     /locus_tag="ROI21_000337"
     CDS             complement(50544..51578)
                     /gene="tqsA"
                     /locus_tag="ROI21_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDX5109313.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYDALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            51990..52355
                     /gene="mdtJ"
                     /locus_tag="ROI21_000338"
     CDS             51990..52355
                     /gene="mdtJ"
                     /locus_tag="ROI21_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDX5109314.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            52342..52671
                     /gene="mdtI"
                     /locus_tag="ROI21_000339"
     CDS             52342..52671
                     /gene="mdtI"
                     /locus_tag="ROI21_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDX5109315.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(52710..53531)
                     /locus_tag="ROI21_000340"
     CDS             complement(52710..53531)
                     /locus_tag="ROI21_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDX5109316.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDRLDQLSQK"
     gene            complement(53631..53714)
                     /locus_tag="ROI21_000341"
     CDS             complement(53631..53714)
                     /locus_tag="ROI21_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109317.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(53807..54142)
                     /locus_tag="ROI21_000342"
     CDS             complement(53807..54142)
                     /locus_tag="ROI21_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDX5109318.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            complement(54539..55792)
                     /locus_tag="ROI21_000343"
     CDS             complement(54539..55792)
                     /locus_tag="ROI21_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5109319.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            55899..56792
                     /locus_tag="ROI21_000344"
     CDS             55899..56792
                     /locus_tag="ROI21_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5109320.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            56927..58147
                     /gene="mlc"
                     /locus_tag="ROI21_000345"
                     /gene_synonym="dgsA"
     CDS             56927..58147
                     /gene="mlc"
                     /locus_tag="ROI21_000345"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDX5109321.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEEAQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSHHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            58272..58967
                     /gene="bioD"
                     /locus_tag="ROI21_000346"
     CDS             58272..58967
                     /gene="bioD"
                     /locus_tag="ROI21_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDX5109322.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(58920..60212)
                     /gene="clcB"
                     /locus_tag="ROI21_000347"
     CDS             complement(58920..60212)
                     /gene="clcB"
                     /locus_tag="ROI21_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDX5109323.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHTMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTIQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLFAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(60371..60985)
                     /gene="dmsD"
                     /locus_tag="ROI21_000348"
     CDS             complement(60371..60985)
                     /gene="dmsD"
                     /locus_tag="ROI21_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDX5109324.1"
                     /translation="MTHFSQQDNFSVTARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQAQSEETRAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(61028..61882)
                     /locus_tag="ROI21_000349"
     CDS             complement(61028..61882)
                     /locus_tag="ROI21_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDX5109325.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAANNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCTGLGCWLCPLIRRREPHVAGLVLGLILILDGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(61884..62501)
                     /gene="dmsB"
                     /locus_tag="ROI21_000350"
     CDS             complement(61884..62501)
                     /gene="dmsB"
                     /locus_tag="ROI21_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDX5109326.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIDELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(62512..64935)
                     /locus_tag="ROI21_000351"
     CDS             complement(62512..64935)
                     /locus_tag="ROI21_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDX5109327.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMNYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(64996..67422)
                     /locus_tag="ROI21_000352"
     CDS             complement(64996..67422)
                     /locus_tag="ROI21_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDX5109328.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGNDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(67621..67926)
                     /locus_tag="ROI21_000353"
     CDS             complement(67621..67926)
                     /locus_tag="ROI21_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDX5109329.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            68034..68744
                     /locus_tag="ROI21_000354"
     CDS             68034..68744
                     /locus_tag="ROI21_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDX5109330.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(68747..69307)
                     /gene="speG"
                     /locus_tag="ROI21_000355"
     CDS             complement(68747..69307)
                     /gene="speG"
                     /locus_tag="ROI21_000355"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDX5109331.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(69342..69683)
                     /locus_tag="ROI21_000356"
     CDS             complement(69342..69683)
                     /locus_tag="ROI21_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDX5109332.1"
                     /translation="MKITLSKRIGLLAFLLPCAMALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            69818..70144
                     /locus_tag="ROI21_000357"
     CDS             69818..70144
                     /locus_tag="ROI21_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDX5109333.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALIWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRA"
     gene            70350..71564
                     /gene="rspA"
                     /locus_tag="ROI21_000358"
     CDS             70350..71564
                     /gene="rspA"
                     /locus_tag="ROI21_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDX5109334.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            71576..72595
                     /locus_tag="ROI21_000359"
     CDS             71576..72595
                     /locus_tag="ROI21_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDX5109335.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            72653..72781
                     /locus_tag="ROI21_000360"
     CDS             72653..72781
                     /locus_tag="ROI21_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDX5109336.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHTCQLFDKY"
     gene            complement(72783..74063)
                     /locus_tag="ROI21_000361"
     CDS             complement(72783..74063)
                     /locus_tag="ROI21_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HDX5109337.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RASVCYAIKTGVFDYAKQFPSSRNLEKFGETRQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLVDNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKHKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(74098..74349)
                     /locus_tag="ROI21_000362"
     CDS             complement(74098..74349)
                     /locus_tag="ROI21_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDX5109338.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(74422..76893)
                     /locus_tag="ROI21_000363"
     CDS             complement(74422..76893)
                     /locus_tag="ROI21_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDX5109339.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCVYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPMKVTADEVNNIMQAANIIQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPESVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEKMEGAEHPHKENTGGNQHHDSDNETGETADHS
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIQFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            complement(76987..77178)
                     /gene="ydfD"
                     /locus_tag="ROI21_000364"
     CDS             complement(76987..77178)
                     /gene="ydfD"
                     /locus_tag="ROI21_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HDX5109340.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQYNIEIPAGL"
     gene            complement(77175..77363)
                     /gene="dicB"
                     /locus_tag="ROI21_000365"
                     /gene_synonym="ftsT"
     CDS             complement(77175..77363)
                     /gene="dicB"
                     /locus_tag="ROI21_000365"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDX5109341.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(77850..78425)
                     /locus_tag="ROI21_000366"
     CDS             complement(77850..78425)
                     /locus_tag="ROI21_000366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109342.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALRIRMCSHNDA
                     EHKYEKQNQTAENFVLEPGETLFKIPVTCPICGFTSEELDDSCNNQETTKYVEDDTEC
                     ARRTIISTSPNSRTNKSHFERVINPLPQTNKKDAGGQKTQWNNGISVKKRRSIKDKQR
                     SANNCKRFIPVINFHVLKRIYWWLRHCLMNP"
     gene            complement(78427..78582)
                     /locus_tag="ROI21_000367"
     CDS             complement(78427..78582)
                     /locus_tag="ROI21_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDX5109343.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(78751..79158)
                     /locus_tag="ROI21_000368"
     CDS             complement(78751..79158)
                     /locus_tag="ROI21_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5109344.1"
                     /translation="MDTRTLGQRVLARRKELRLTQREAARLAGVAHVTISQWERDETQ
                     PVGKRLFALADALKCSPTWLMFGDEDKAPVPAQELHVETELTPNHKELIELFDALPSS
                     EQEALLSEMRARVENFNKLFEEMLKARKNKSIK"
     gene            79239..79466
                     /locus_tag="ROI21_000369"
     CDS             79239..79466
                     /locus_tag="ROI21_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDX5109345.1"
                     /translation="MLKVDAITFFGSKTKLANAAGVRLASVAAWGILVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            79450..79971
                     /locus_tag="ROI21_000370"
     CDS             79450..79971
                     /locus_tag="ROI21_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109346.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRISDFRLY
                     REITDGKSITCMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            79952..80917
                     /locus_tag="ROI21_000371"
     CDS             79952..80917
                     /locus_tag="ROI21_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109347.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDDGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDPTENTT
                     EITTENKNTFRPEASQPDPQTAEQDFLIRHPGAVVFSAKKRQWGSQEDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTVWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            80958..81356
                     /locus_tag="ROI21_000372"
     CDS             80958..81356
                     /locus_tag="ROI21_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDX5109348.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKTSVVDPDLIWSLPDG
                     EIRRYDRHQNIICCECRKSEVMQRILAFYQ"
     gene            81559..82224
                     /locus_tag="ROI21_000373"
     CDS             81559..82224
                     /locus_tag="ROI21_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDX5109349.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASRIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYDKE
                     EKE"
BASE COUNT        20789 a        20060 c        21085 g        20663 t
ORIGIN      
        1 cgattatggt tcagcgtatt actattgcgc cgcaaggccc ggagttttcc cgttttgtga
       61 tgggctactg gcgattgatg gactggaata tgtccgcccg ccagctggtc agttttattg
      121 aagagcatct ggatctcggc gtgaccaccg tggaccatgc tgatatttat ggtggctatc
      181 agtgcgaagc ggcgtttggc gaggcgctga aactggctcc tcacctgcgt gaacggatgg
      241 aaatcgtcag taaatgcggt atcgcgacga ccgcgcgtga agaaaacgtc attggtcatt
      301 acatcactga ccgcgatcac atcattaaga gcgccgaaca gtcgctgatt aaccttgcga
      361 ccgatcatct ggatctgctg ttaatccacc gaccagaccc gttaatggat gccgatgaag
      421 tggcggacgc gtttaaacat ctgcatcaga gcggcaaagt gcgccatttt ggcgtatcca
      481 acttcacgcc cgcgcaattt gccctgttgc aatcacgcct gccgtttacc cttgccacta
      541 atcaggtgga aatatccccg gtgcatcagc cgttactgct ggatggtacg ctcgaccaac
      601 tgcaacaact gcgtgttcgt ccgatggcgt ggtcctgcct tggtggtggt cgtctgttta
      661 atgatgatta tttccagccg ctgcgtgatg aactggctgt ggtggcggag gagttaaacg
      721 cgggctcgat tgaacaggtg gtttacgcct gggtattacg tttaccgtcg cagccgctgc
      781 caattatcgg ctcaggtaaa attgagcgcg tacgggcagc tgtcgaagca gaaacactga
      841 aaatgacccg tcaacaatgg tttcgtatcc gtaaagcggc actgggttac gacgtaccgt
      901 aagcgctttt aggaatagcc gccgttcaaa aatgtgtcac tggtttacac ttattcagga
      961 atgcacaatg aacggaggtc ctatgaaacg ttttagtctg gccattctgg cgctggttgt
     1021 tgcgaccggc gcacaagctg ccagtgaaaa agtcgagatg aacctcgtca cgtcgcaagg
     1081 ggttgggcag tcaattggta gcgttaccat tactgaaacc gataaaggtc tggaattttc
     1141 gcccgatctg aaagcattac ctcccggtga acatggcttc catattcatg caaaaggaag
     1201 ctgccagcca gccaccaaag atggcaaagc cagcgccgcg gaatccgcag gtgggcatct
     1261 tgatccacaa aataccggta aacatgaagg gccagaaggt gccgggcatt taggcgatct
     1321 gcctgcactg gtcgtcaata atgacggcaa agccaccgat gccgtcatcg cgcctcgtct
     1381 gaaatcactg gatgaaatca aagacaaagc actgatggtc cacgttggcg gcgataatat
     1441 gtccgatcaa cctaaaccgc tgggtggtgg cggtgaacgc tatgcctgtg gtgtaattaa
     1501 gtaattaaga ggccagcgta ccttgcggcg gtgcttgctc aagttgcgaa agcgagcagt
     1561 acagccgcca gacaattgcc gccagctcac gggcggcagg ttgatgatga cgggcaaggc
     1621 tgtagcaaat ccgctgtaat tcttcaagtg tggccgccag tgatttttgc tgaacgccac
     1681 gctcactcat cactccccgc aacagtgaaa tacagttatc ccgtactcgt gataacggat
     1741 cggaacgcga ttcccagtcg cgcaattgcc agacaacatg agaacagttc agcagcacta
     1801 caccccagcg taataaccaa cggcgagcca gcgcatcctg gctgttactc aactgactga
     1861 cgtgatgata agtgagcgat tcaaattcgc tttcactcag tgttggatgg cggcttagtt
     1921 gatcgacaaa atcccggcgc agcgcgcgta tatggcgacg gcttttacga gcatccgatc
     1981 ctggacgcaa aatggcgaac gctaaccacg ccagcgcaac gccaacgatt tttgccagat
     2041 tatcgttaag aaaatcagca aaatcataca ccgggggatt agtgacagcg ataaacgaac
     2101 ccataaaaac aatcagttgc ccccacagtg cggcaaattt gggcatctgc aatttaagaa
     2161 gctgcattgt cgccagcagt ggaaagagaa acagtaaaaa ttgccacaga tcgctaatct
     2221 ggaccatcag gccaaatttg accacaaagc tgaatagcga aagtaacacc agcgtacgca
     2281 tcagcagtga caacgactta aacggtgctg cgacggcgga gtagagtacg cagctaattg
     2341 ctgccagcgt taatgcattg gcaccggcat cccattgcga agcaatactc catgtgccaa
     2401 tcatcatcaa cgtacaaaat gtacgcagtc cgctccacat agcttcggcg ttatcggtat
     2461 gacgagccag gccggatgtc catgggagtc tggcatgatc atctacaccg ctttgcagac
     2521 gatgtaattc ctgactactt tgcagatata ggcggcaaaa ataacgtagt cgctgccaga
     2581 aggcgacgtg ccgatagtcg gcgacgttgg tcgggcgtag tggggcgata atacgtgcga
     2641 cggtgtaaat atctgtttgc gaactggcga gcgccgttag caactgttcg agaatttctc
     2701 gtgtggcaca tggcggtgag ggccagttga gcaacatacg tcgcaggctg gagatgacac
     2761 tggtcatacg taattgctgg tggagtagcg cattaaggcg cgcgttttgc tggcgaaaac
     2821 gatagtggct ccagaaagcc tggatacgca gcaaattcat ggtcagtatc tgcccaatca
     2881 ccccctcatg tgctgcacga atggcatcgg ttgtttctgg ctgccagagt aaactggcat
     2941 gttccagtaa tcgggcgtgc atgtttttca atgcggttaa aagggcagta gcatcggaac
     3001 tgctcggcag gatcatcatc atcatgccgc cgcacaaaat accgacaatc acctcgcaaa
     3061 cgcgcgcctg agcgatatcc cacagctggc tggcctcagt aatattaacc atcggaaagg
     3121 cgatgatggc agccgtgtag cccgccagtt gaaatgcata cgcgacatta ttcgtgaagt
     3181 gcgcacaggc ccaggtacaa aagccaagcc acgccgccat gctcaaaaga aaaaaccacg
     3241 gctcattgag cgtatgtccg gcaagaagca gtgccgcaat ggctccgagc aaactgccag
     3301 cgatgcgtcc gaggcttttg ctgatgacac cgccaacggt gggaaagcta accacggcag
     3361 ccgaggtcat cgcccaatag ggttcatcca gatttaaata ataggcaacc gtcagcgcca
     3421 gacacatggc aatggtattg cgtaacgcat aacgccattg cgccagcgtg gccctgaacc
     3481 agggcaaatt gcgcaaggac catgacgatg cgttcattat tgacctacgg caatgctaca
     3541 ggtggtgccc gacaccagcg tgacatcgcc tgggacttta tccagcgcaa agcggacggg
     3601 aacgcgttgg gcgaggcgaa cccaggggac gttaggtttc acatccggaa taaggcttga
     3661 gtcactttca acgctctgat cataaatagc ccgaccgata ctggaaacgt gaccctgtaa
     3721 cgttttgtta tccctataga gggttatttg tgctggtgca ccttcgcgga tatggcgcaa
     3781 cttggtttct tcaaaataac caatgacgta aaacgagtgg ctatcgacaa gagcaaacag
     3841 cggtttcccg gtgtcagcgt aatcgccgat gcgggtagtg aggttagtca cccatccact
     3901 caccggggcg cgaattttcg tttgcgccag ttgccattgt gcctgtttca gcgtcgcctg
     3961 tgcggcatca acactggcct gcatcgcttt aacattgagg ttagcggtat cgagttcttc
     4021 ggcagaaata aaattttgtg agagatgacg acgacgattc gcttcgttat tggcttttgc
     4081 gaggtcagat tgcgctttcg tcagctgagc ctgtgcgttc agctcggcga tctgaaaggg
     4141 cgttttatcg atcgtgagca gaagatcccc cgcgttaacc agttggttgt cttttatatt
     4201 cagctcaaca atacgtcccg acacctgagg ggtgatagaa acctgttcag cgcgtatttt
     4261 cccgtcgcgt gtccacggag attgcatgta ataattccac agccaccatc cggcaagaac
     4321 cgcaactacc gctacaatga ttgttgagaa atacttaatt gttttaattg acatagttac
     4381 cacgcaatca gtattgccag ggcaaggcaa acgcaaatcg caaacagcga taaatccatt
     4441 aacaagggat gccagatgtc accggcgtag atccagccac gaagcaggcg gtgtacgaca
     4501 agccagatga cgaatccaaa cgcgaaggct ttgaaaaacg gaggaaagta gacggacgca
     4561 ccgaacacca gatcttgtaa gggcaatcct gttgcattga gcataaactt cacaaagtag
     4621 attcctttac gaccgaatat attgcgttag attaataaat attctttaag tgcgaaaaat
     4681 ttacgcgcaa tttctgaaaa atatgtctga cggtaaccaa atgcagcaat acatttgttt
     4741 tagcaataca attgctgcac actattctaa aagccgcata atatcttagc aagctaatta
     4801 taaggagatg aaattggaat cgccactagg ttctgatctg gcacggttgg tgcgcatatg
     4861 gcgtgctctg atagaccatc gcctgaaacc gctggagtta acacaaaccc attgggttac
     4921 gttacacaat atccatcagt tacctccaga ccagtcgcaa attcaactgg caaaagcgat
     4981 tggcatcgag cagccatcac tggtccgtac tctggaccaa ctggaagaaa aagggttaat
     5041 ttcgcgtcaa acttgtgcca gcgatcgtcg ggctaaacgt attaaactga cggaaaaggc
     5101 agagccgctg atcagcgaaa tggaagctgt tattaacaaa acccgcgcgg aaattttaca
     5161 tggcatctcc gcagaggaac tggaacaact gattaagctc atcgcaaaac ttgagcataa
     5221 tatcattgag ttacaggcca aagggtgaaa tgaagggggc gtgtggtcag taacctgacc
     5281 acacgcaaac ttacttattt agcgcgggga aacggtcacc tgactgccat tgctggccag
     5341 tacgacacgt tggcccggag agaaacgagt gttgccttgt ttctgtacca ccatgatggt
     5401 attaccatcg tctttacgaa tttccagctc gacaccctgc gttttgttca ttgcactctg
     5461 tacgccctga ccggctacgc cacctgcaac agcgcctgct gcagtagcca gagaacgccc
     5521 ggttccgcca ccaacagtat tccccaggaa accaccaaga acagcaccgc caattgcacc
     5581 gataacgttg gaatcatcac cgccctgaat ctgtaccgga cgtacgttaa cgatggtgcc
     5641 atagctgaca ttctgtactt gtttcgcttc agaagcggta taaacatccc ctgacagggt
     5701 gtcgttatta acacaaccga caagagacag acctaccatt gaaacaacca atacgcgttt
     5761 aatcattgaa aaatctcctg ttcaccatga aacgccacgc aagcatccct catggtcaaa
     5821 gtatatggca tatgtgaatc catggttaca tgttctacca aatatgagca aatcataatg
     5881 aaagtaggat caaccaatta taaacaaaac attcatgatg attattgatt tgaaggtaat
     5941 gttttactac caacggtaaa aatcggtcat tccgcatagt attcacgaaa agtttatgtt
     6001 tgagtggtgc gcaaacgtca gattgaaaaa ggatgaacta tgaaatcggg ccgctttatt
     6061 ggcgttatgt ctggcaccag ccttgatggt gttgatgttg tgttggcgac aattgatgaa
     6121 caccgggtcg cacagctggc aagtttgagc tggccgatcc cggtgtctct gaaacaggct
     6181 gtactggata tttgccaggg ccagcagctt acactttcgc agtttggaca gcttgatact
     6241 caactcgggc gactttttgc tgatgcggtc aatgccttgc ttaaggaaca aaacctgcag
     6301 gcgagagata tcgttgcgat cggctgtcac ggtcaaaccg tctggcatga accgacgggc
     6361 gtggcaccac acactttaca gattggcgat aacaatcaaa ttgtggcacg caccggaatt
     6421 acggttgtcg gtgattttcg ccgtcgcgat attgccttgg gaggacaagg cgcaccgctg
     6481 gtacctgcgt tccatcatgc gctgctggct cacccaaccg agcgacgaat ggtgctcaat
     6541 attggcggca tcgccaatct gtcactgctc attcctgggc agccggtagg gggctacgat
     6601 accggccctg gtaacatgct gatggatgcc tggatctggc gtcaggcagg taaaccttac
     6661 gataaagatg ccgagtgggc acgggcgggt aaagttattc tcccactgtt gcaaaatatg
     6721 ctcagcgacc catatttctc gcaacctgca ccgaaaagta ccggacgcga atactttaac
     6781 tacggctggc tggagcgcca tttgcgccat tttccgggtg ttgatccccg tgatgtgcag
     6841 gccacactgg cagaactcac cgccgtgacc atttctgaac aagttttgtt gagcggtggc
     6901 tgtgaacgat tgatggtatg tggtggagga agtcgtaatc cgctactcat ggcgcgtctg
     6961 gcggcattac tgccaggcac agaagtcacc accaccgatg ccgttggcat tagtggcgat
     7021 gacatggaag cattggcttt cgcctggctt gcctggcgta cgctggcggg attaccagga
     7081 aatctgcctt ccgtcactgg cgcaagccag gagacggtac tgggggctat tttccccgct
     7141 aacccgtgat atcagagtta actgaatttt tcttaccgcg tctgccgtta cacttggaac
     7201 gttagggagg gcgtattgcc ctccggacca ggaaagtctt cgggatatgt ctatgaaaaa
     7261 actgttactg atctgtttgc cggtgcttct caccggctgt agcgcgttca atcagctggt
     7321 tgagcgcatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
     7381 actgaataat ccgcgccagg aggtcagttt tgtatacgat aatcaactac tgcatctcaa
     7441 acagggcatt tcagcctctg gcgcgcgtta cactgacgga atctatgttt tctggtcgaa
     7501 aggcgatgaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
     7561 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
     7621 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
     7681 aatacaccaa aggcgggttg cgccgccgcg atcttcctgc cgagccatta accctttttg
     7741 aacgctggct ctctcaggct tgtgacgcca aactggcgga ccctaccgcg atggtggtcg
     7801 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
     7861 aaaaaggcat ggtgttttac accaacctcg gcagccgtaa agcacatcaa atcgaaaata
     7921 atccgcgcgt tagcctgctg ttcccgtggc atacccttga gcgccaggtg atggtgatcg
     7981 gtaaagcgga acgactttcg actctcgaag tgatgaaata ttttcatagt cgcccccgtg
     8041 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
     8101 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
     8161 gtttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
     8221 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
     8281 ttgccccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     8341 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     8401 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     8461 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     8521 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     8581 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     8641 cgcgacgggt ctgattggcg acccgagctt caaagctgct gagcgtaagc tgaacaccga
     8701 agaaactgtt caggaatggg tggacaaaat ccgcaagcag gttgccccgt tcctcgattt
     8761 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     8821 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     8881 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     8941 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg gtgtggtgct
     9001 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     9061 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     9121 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     9181 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     9241 cttcaccttt atgagcattg aagagatcaa cgccctggaa gaagaagata aaaacagcgg
     9301 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     9361 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     9421 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     9481 aaaaggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt ctcgtggtca
     9541 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     9601 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     9661 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     9721 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     9781 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     9841 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     9901 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     9961 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
    10021 gtgaacatat cctcggtatc gttcgtcagg tgaaagcagc gaatccgcag gcgaaatatt
    10081 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
    10141 agtttcatgt gcgtcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
    10201 tggagatact ctgtgagcat ccggtaaata acgtcgaaga agcggttctg gcagcgcgtg
    10261 aactcattgc gcaagggcca caaattgtgt tggttaaaca cctggcgcgg gctggctaca
    10321 gccgtgaccg ttttgaaatg ctgctggtta ccgccgatga agcctggcat atcagccgtc
    10381 cgctggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
    10441 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
    10501 cagtatacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggttg
    10561 ctgctcagga tcgtattgcc aaaccagaac attatttcag cgcaacaaag ctctgaaata
    10621 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
    10681 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
    10741 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
    10801 atcagcaatt gtaaatcgtt gcccgcagat ccagtgctca tccttcagag catcgttcac
    10861 atattgcagc ttcttctcca gctgcgcgcg aactgttggt ttgtactctt ccggtgtatc
    10921 agggcgaaac agaggtgtga agcctttatg cagctcggtg gcgatgtaat tcagccattc
    10981 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
    11041 gtcggcaaga tactgcataa ttgctacgcc ttccgtcagc aaagtaccat cgtccagcag
    11101 caatgcaggt acctgtccct tagggttaac ggcaaagtaa tcgtcaccgt tttcgagacg
    11161 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
    11221 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
    11281 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta acgaaaagtt
    11341 agcggcttgt aaaaaagttt ccctgaattt tacgctacag cagctttcgc cgctttgtct
    11401 gcagcgtcat cctgcgtcat gcggtgcagt ttcggcgcgg tcagcagcat cagtactgca
    11461 ataacggcag tagcgacacc aatctgcaag aatacgcgac catagacttc cagtgacatc
    11521 agtggatcgg taacgttatc cggcacagcc atcatacccg caacataacc accaatcagg
    11581 tttgcaccgg cagtggtgag gaaccagcta cccataatga agcccatcag acgctgcgga
    11641 acgagttgtg caaccattgc cagacccaga ccagagatca taagttcccc gatactctgc
    11701 aggccatagc tcgcgaccag ccagcttaca gacacgatac ctgcgtcaga cgcgaatttc
    11761 gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat tgcaaacttg
    11821 gttggcatcg gcagggtatc gcccatcttg ttatagatag cggccagaat cggactaccg
    11881 atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc cagacccaga
    11941 atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat ctggctgtac
    12001 agcacgaaga agataatggc ttcgagcatc aggatgaagg caacgatcat tttacgacga
    12061 gcagcacctt tcatcgcgaa ggcttcttta ccgaagataa ccacaatacc gaaggcaaca
    12121 acgcccagcg ccatacgcgc aacttcctga ttgtgcagca gccaggtggc gatagcgatc
    12181 agtgccacaa caccaataat ggtcagcagc aggttacggt agttgatagg ctcgaagtct
    12241 ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac gatagtgatc
    12301 agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc ggccagccac
    12361 ggggtggcga tcatagagaa gaaagagccg atgttgacgg acatgtagta catggtgaat
    12421 gcaccgtcca gacgcgggtc gtttttctca tagcatgtag aaagcagaga agacgggtta
    12481 gctttaaaca ggccgttacc gaccgcaata gccgccatcc ccatataaac gataccggcg
    12541 tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc gccaagcata
    12601 attacgcgtt tagtacctag taccttgtca cctaaccagc cgccgatagc gaccagacca
    12661 taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga catacccagt
    12721 tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa acgttcccat
    12781 aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt caaactgacg
    12841 ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac atcccatatt
    12901 aacgggggtg ctttctcgcg ttatgcctaa aaaacatacg cgcaattgtt atagtgggag
    12961 gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt gtaataatct
    13021 gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc agcgttaaat
    13081 ttttcaaaaa acgtaacggc agattttttc actaccctga gagggggatg atggcttaac
    13141 ggcgatatgt tctgctattt agcgttttta ctagtgagat agtccagttt ctgaaaaata
    13201 gccagtgtaa tgttttgtag gtcaatattt gttgcgtgtg taaatttaaa taattttaac
    13261 ctagttcttg caatgttttt acattgccac agcatcgtga tcttgatcac gaatgaatag
    13321 aaaatttcgc tattaaaaaa acaattaacc tgcatttttg gttatccgca aacggattac
    13381 ctgatgataa cgctaagcaa tggcatcatt gacgttaaaa agatgtttta aaagaggata
    13441 aagaaaggtt atcaatgggg taatcggtgt tacccctttt cttcagatgt caactttctc
    13501 tttgtattca caaagatctt caataataca agagccacag cgaggcttgc gggcaataca
    13561 ggtataacgc ccgtgcagga tcaaccaatg gtggcagtcg actttaaact ctgccggaac
    13621 cactttcagt agcttttctt ctacctgttc gacgtttttc cccggcgcaa attgagtacg
    13681 attacaaacg cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga atgcagtgtt
    13741 taatacgacg ttggctgttt tacgacctac accaggcagg gcttcaagcg cagcacgatc
    13801 ttccggaacc tcgccattat gctgttccag taagatacgg caggttttga tgatattttc
    13861 tgctttgctg ttataaagcc caatggtttt gatataggtt ttcacccctt cgacgcccag
    13921 ttcaagcatc gctgcaggtg tattagccac cgggtagagt ttcgccgtcg ccttattaac
    13981 actgacatcg gtcgcctgag cggaaagcag tacggcaatc agcaattcaa aaggcgaact
    14041 gaaattaagc tcggtggtgg gatgaggatt gttctcacgc aggcgagtga ggatctccag
    14101 gcgttttgct ttgttcatca gacattccct gtttcaccgt ttggcaatgc acgttcggca
    14161 gctgcttcag tacggcgctt tttcattctt tcatcaatca ggtatttccc tgccagcatc
    14221 agtcccaggc caataaatgc acctggtggc agcatcgcca gcaggaaagg ggagtcggtg
    14281 tggaaaattt ccacgcgtaa tacttttgcc cagctaccta acagcgcatc tgcaccgtca
    14341 aacaacgtgc cattgccgat aatttcgcgt agtgaaccca gcacgaacat ggcgcaggtt
    14401 gcgcccatac caattgaaaa gccgtccagt gccgaaagcg ccggaccttt tttggcggcg
    14461 aaggcttcag cgcggcccac aacgatacag ttagtgacaa tcagcggaat aaaaatccct
    14521 aatgattgat acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc gctgaccacc
    14581 gaggcgatga tcatcacgta aatgggaatg cggatctcgg ctggcgtcca gtgacgcagc
    14641 gtcgaaatgg tcaagttggt cagcgtcagt accagcgtag tcgcaagtcc taaacccaga
    14701 gcgttagtgg cagtggacgt gaccgccaat agcggacaga ggccgagcag ctggaccagc
    14761 gcagagttgt ttttccacaa cccctgaaca ataacgtctt taatttcgct cacgctttat
    14821 tctccacagg caggaagttg agaaagttgt gacggtaacg tctgagcgta caatccggcg
    14881 cgttttaccg cattaaccac cgcgcgcgga gtaatcgtcg cgccggtgaa ctggtcgaaa
    14941 tcaccaccat ctttcttcac cgcccagtgc gcatcatccg caccactgat ttttttaccc
    15001 gcaaaatggg tgatccagtc agaaaggcgc agttcgattt tatcgccaag ccctggcgtt
    15061 tcgtggtgct ctgtcacgcg cgtgccaagt accgtgccgt taaaatcggc tcccaccagc
    15121 agctgaatcg caccggaata gccatctggc gcggttgttt ccagaacggc ggctaccggt
    15181 ttgtcatcct gtttggcgat gtaaacccga tgctcacctt tacctaactc tggcgcagtt
    15241 accagatagc aactctgtgc cagcgcattg ttatagcgtt cggctggcag cacctgatca
    15301 aataacgctt tttgttgcag actggcctgt tcagcaatcg tcgttttggt catctggttg
    15361 atggccgcag ttaaccctgt agaacccgct gcaaatagtg ccaacgtaat gccgtgtttt
    15421 cggatagttt tcagcatggt ttaccctttg cgatggccgt agacgcgcgg acgcgtgtag
    15481 taatcgatca gaggaaccgt gatgttcgcc agcaggacgg caaaagccac gccgtcagga
    15541 tagccgccga aactgcggat caaccagact aataagcccg ccagcgcgcc gaaaataaga
    15601 cgaccacgat tggtcgtaga agcggtaacc gggtcagtca aaataaagaa tgcgccgagc
    15661 atagtcgcac cagacagcag atgaatttgg ggtgatgcca gtgtttctgg tgagaacaac
    15721 cagcccaacg ttgcgcataa cgccagcgtt actaagaagc tgaggggaat atgccagcga
    15781 atcgctttct gccatagcaa ccacacgccg ccagccagcc aggcgagatt tacccattgc
    15841 caaccaacgc ccgccagcat accgctgtag atcggatatt gcataatctg ttcaaccgaa
    15901 tgaccggcac ggacagaggt tttaaatgta tccagcggtg tcgcctgact aatgccatca
    15961 atacctaagc gtagcgtgtt catatcacca ccactggtgg tatgtccgct gaaaataacc
    16021 tgtatggcgt cgataaaacc agggatgttg accgcaattt catgtggcgg taaccagctg
    16081 gtcatctgca ccgggaagga gatcagtaag accacataac caatcattgc cggattaaac
    16141 gggttttgcc ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa caccgtacct
    16201 agcacgacca tccaccatgg cgcgaggggg ggaatactta ccgccagcaa taagcctgtc
    16261 agcaatgctg agttatcttt caacgttgcg gctaccgact gcttgcgtag tttgagtacg
    16321 agagcttcgg ctaacagagc actgaccgac gccaacagga tctgaacgag agtaccccaa
    16381 ccaaaaaacc acagttgcgc tgcgattcct ggcaccgctg cgagcaacac caacagcata
    16441 atgcgcgatg tctggcgctg gttatgggta taaggggagc tagctattct gaataccatt
    16501 tagtcctcgt ttacaacctt ttgctgggcg gcttttttgg cctgaacgcg ggcaatagcc
    16561 gcggcaactg ccgctttgcg cggatcaacc tgttcttctg gctccgcatt agcctgttgc
    16621 tgttccagtt tgcgcgcttt ggcacgggca atagcggctt cgacggcagc tttgcgcgga
    16681 tcgacctgtt cttctggttc cgcattagcc tgttgctgtt ccagtttgcg cgctttggca
    16741 cgggcaatag cggcttcgac ggcggctttg cgcggatcga cctgttcttc tggttccgca
    16801 ttggcctttt gctgttccag cttgcgtgct ttggcgcggg caatagcggc ttcgacggcg
    16861 gctttgcgcg ggtcaacctt ttcttctggc tccgcattag cctgttgctg ctccagcttg
    16921 cgcgctttgg cgcgggcgat agcggcttcg acggcggctt tgcgcgggtc aacctgttct
    16981 tctggcttcg cattagcctg ttgctgctcc agcttgcgtg ctttggcgcg ggcgatatcg
    17041 gcttcaactg cggttttacg tggatcagca acggttgctg cgtcgttagt ttgctgcagt
    17101 tctgcctgtt tcgctctggc ttgcgcttta cgggcttctc gtgctgcaat aattgcactg
    17161 ttatccgggc gttcgcccgc tttaatcaca atcggctgtg tagcctgggc ctgtttctct
    17221 ttcacccgcg ccagagcggc agcaatcgca tctttatctt tggctgcagg ttgaacggct
    17281 gcgctcttat gtcgttcaag gcgagccgct ttttcgcgct ccagacgagc ctggcgcgct
    17341 tcgaaacgcg ctttggcttc tgcggcgcgc ttttcttcct gatgaatagc cgcaatttca
    17401 gctttttcct gacggaaata ttgcaccagg ggaatattgc tcgggcaaac ccacgcgcaa
    17461 gccccacatt caatgcagtc ggcaatgtta tgcgtggtag ctttatcgtg ttgctgacct
    17521 ttgctgaacc agtacaactg ttgcggcaaa agatccgcag ggcaggcgtc agtacaggcg
    17581 ctacaccgga tgcagctttg ttcttcctgt ggttcgccaa gctcattggc agagggagcc
    17641 aacagacagt tggtaatctt tacgaccggg acatccagcc atggcaaggt aaagcccatt
    17701 agcgggccgc ccataatcac catttgatcg gcagaggggc agaatccggc atcattcaat
    17761 aaatgacgca ctggcgtacc cagccgtgcc cagacgttgc ccgggcgtgc gattgcttcg
    17821 ccagtcaggg tcacgacacg ctcggtaatc ggttcgccat caataacggc acgtttcact
    17881 gcataagcag tgccgacgtt ttgcattaat acgccgatat cggatgaacg cccgccatgt
    17941 ggaacctgct tcccggtcag aatgtaggtt aattgtttag caccgccaga aggatatttg
    18001 gttggaatca cccgcagaga aatatcgtga gagtccgcca gcaccgcgcg cagcatggaa
    18061 atcgcctgcg gtttgttatc ttcaatgccg ataagaattt cgcgtggctg cagaatatgc
    18121 gcaagaatgc gaataccctc tacgacctga gccgcgcaat cctgcatcaa acggtcatcg
    18181 gcggtaatgt acggctcgca ctcagcagcg ttgataatca acgtttcaat cttatctccg
    18241 ccgccctgca atttaacgcc tgtcgggaat cccgcaccac ccagcccggc aacaccaaac
    18301 tgatgaatgc gctcgattaa ctcttcgcga ctgcgagagc gataatcatc ccagccgtcg
    18361 cgcgggatcc agcagtcttc gccatcggca tcaataatca cgcttaattc agctaaagct
    18421 gaaggatgag ccgtagagtg gggcgcaata gccgtaacgg tacccgaggt gggcgcgtga
    18481 actggcagca ttttgccgcg tccacgggta agcggctggc cgcgcagtac tttatcgccg
    18541 acgctaacgc acaactcacc ttcagcgcca atatgttgtt tcagtgggat gacaaaacgc
    18601 tgcgccagtg gtacctggcg caggggcgta ccgttggact gggttttcat ctccggtgga
    18661 tggatgccgc cgttgaaatc ccagatttta ttttttctga atgcagagaa taacttaagc
    18721 atggtgttcc acgggaatga tacgcacggg aatggtgttc agatcccatt tccaggagtc
    18781 aggtgtttct gcaaccggtt gcaacgagat gcagtgcgtc gggcacggat caacacataa
    18841 attgcagccc gtacagagat cactcattac cgtatgcatg gcacgggtag cgccaacgat
    18901 ggcgtctacc ggacacgcct gaatacattt agtgcagcca atacagttat tttcatcaat
    18961 aaccgccacc atccgcgcag gcgttagctc ttgcgcttcg ccatccagcg gctgtggctc
    19021 gacattaagc aactcagcaa tttttagcat cacagcttcg ccacctgggg cgcaacggtt
    19081 gattttttca ccgttacagc tgatggcttc cgcgtaaggg cggcagccgg gataaccgca
    19141 ctgaccacac tggctctgcg gtaagatttc gtcaattttc tcaacgaccg gatcgtcttc
    19201 caccgcaaaa cggcgggagg cataacccag aatggcgcca aacgccaggc ccagcaggct
    19261 cacggcggca acggcaatcc agatagcatt cattacaact tcaccaaacc actaaagccc
    19321 ataaaggcca gagacataag acctgcggta attaacgcaa tggcattacc gcgaaaaggt
    19381 gctgggacat cagccacagc aaggcgttcg cggatggcgg cgaagagcac catcaccagc
    19441 gagaaaccga cagcggcgga aaaaccgtac agcgccgact gcaagaaatt gtgcccgaga
    19501 ttgatattca gcaacgccac gccgagcact gcacagttgg tggtgataag cggcaaaaaa
    19561 atccccagca gacggtaaag caccgggctg gttttgcgca ccaccatctc ggtgaactgc
    19621 acaaccacag caatcaccag aataaacgcc agggtgcgca ggtaaatcag attaagcggg
    19681 atcaaaatcc acgtatcgat aagccaggcg caaatagacg ccagcgtcat cacaaacgtt
    19741 gttgccagcc ccatgcccat cgcggtttcc agctttttgg aaactcccat aaacggacag
    19801 agaccgagaa acttgaccag tacaaagtta ttgaccagta cagttccgac aaacagtaac
    19861 aggtagtcag tcatttttcg gcctgaaata aaaaagccgc ctattatccg ttaatccaga
    19921 gcaggcgaca acaggttaac tgtaaggtta ttacggattc acaaacgtcg ctttcacccg
    19981 cgacgagcgt ttaaaatagg ggacgatcaa tgcggttgcc aacaaagtaa acagtaactg
    20041 gcgaacggct atgccatctt ccacgggtga aaaagcgaac gcttttactg ccagcaatac
    20101 ggaaataagc agccagataa tatagtgttt aggaacacaa cgccgacgtt taaagaaagc
    20161 gatggtcaac cacagtgtgt aataccacat ggcgatagcg gtaataaaag aaacgcccca
    20221 taagatttgt gtcgtcagtg cttgttcgcc gagcgtttga aatgtttgcg gagaagataa
    20281 cgcagcggta tacagcaaca acgccagcgt cgtacttaac agagcgacta aaagccaggc
    20341 caatgggcca agcaaccagc ctccaatacg ttgtggcgtt gtggtggtca taggttctcc
    20401 agaatagcga cgcaaacagg caaatctaat tttaggggcg ttattataag gcttttataa
    20461 ccgatcgcta ctttctttat tggacatagt gccagacgga cttcggtacc tggccgaggt
    20521 caaacaaacg cccgccagaa accagctctg cgcgacggtg atcggcagca cgatacatat
    20581 tgatcagttc ctgatcgtcc gtcagggtgt aattaagatg gtcgtagagt ttttccagac
    20641 tttcgagtga actgattttg cgaaatttta ataagtagtc ctgaacagtc ataaaaggtc
    20701 cataaaataa ttaataccaa aggggtaggt attattagtg atgataaaat ttcaccaaaa
    20761 gtgaacgctt gtgttattac aataataaga atgcgctgat ggcaaatatt cagggatgaa
    20821 aaactgctct gaaaagagta acgcgcttta ctatctctga caatcaccaa caacatcgaa
    20881 caagataata aattcctggt ttaatatccg acaagtgaaa acatgcaccc ggacgggcag
    20941 catgtcgctc tacaagcgca gagcttactt gtgttgtacc gtagtactct gttcaggtgg
    21001 ctgatagttg tcaatgtgac tcgccacgcc aagaagaatg actgaaacga caagaacgat
    21061 ccaacctgtt aattcaataa gacgattcat tacagcccac atctctgttg attgatccat
    21121 taacttcagg gggtaaatgt tacttagcaa taatagctcc agcaagattt ttactgaggt
    21181 ttttgcgata tttagctttt gtcgttggaa aattcgctat ttttttgact taagttaaac
    21241 aacatccctt attgctggca ggttattaaa ctgttgagcg agggtaaagg atagtgtcaa
    21301 atagccatca tacttcaatg agaggcaatg acatgagcga caacatccgt gttgggttga
    21361 ttgggtatgg ttatgcgagc aaaaccttcc atgcgcccct gattgcgggc acgcccgggc
    21421 tggaactggc ggtaatctcc agcagcgatg aaacaaaagt aaaagctgac tggctaacgg
    21481 ttacggttgt ctctgagccg aagcatctgt ttaacgatcc caatatagac ctgattgtca
    21541 tccctacacc caacgatacc catttcccgt tagccaaagc ggcgcttgag gcgggtaaac
    21601 atgtggtcgt tgataaaccc tttaccgtga cactgtcaca agcgcgagag ctggaagcgc
    21661 tggcaaaaag cctggggcgt gtgctgtctg tattccataa ccgtcgctgg gatagcgatt
    21721 tcctgacgct aaaaggtttg ctcgcggaag gcgtactggg tgaagttgct tactttgagt
    21781 ctcattttga ccgcttccgt ccgcaggtgc gcgatcgttg gcgtgaacag ggcggccctg
    21841 gcagcggtat ctggtacgat ttagcaccgc accttcttga tcaggccatt acgctatttg
    21901 gtttaccggt tagcatgacg gtagatttgg cacagttacg gcccggagcg cagtcgaccg
    21961 attatttcca cgccatcttg tcctatccgc agcggcgagt cattttacac ggcaccatgc
    22021 tggcagccgc tgagtcagca cgttatatcg tgcatggatc ccgaggcagt tatgtgaaat
    22081 atggcctcga tccacaggaa gaacgtctga aaaatggcga gcgtctgccg caggaagact
    22141 ggggctacga tatgcgtgat ggcgtactta cccgcgtgga aggtgaggaa cgtgtcgaag
    22201 aaacgctgtt gacagtacca gggaattatc cggcttacta tgcggctatt cgtgatgcgt
    22261 taaatggcga tggtgaaaat ccggttccgg caagtcaggc aatccaggta atggagttga
    22321 ttgagctggg catcgaatcc gccaaacatc gcgcgacgct gtgccttgcg tgagacgctg
    22381 tgccttgcgt gaaaagggga tggtgcaatt tgcaccatcc atttttgtta cttcgcagcg
    22441 actttttctc gcagtgcgcg tttttcctct gcgctgagga aagccatttc cagaccatta
    22501 atctgcgcct ggcggatttg ctcgcgggat aacccagcag ccggcgcggc aacggtatat
    22561 tcgtgaatga tatccactcc ctgtacgccg ggatcgtcag tgttaatgct ggcacgaatg
    22621 ccatgctcaa ggaacgtttt cagcggatgt gccgccagct ctgccacggt gctggtctga
    22681 atattggagg tcagacagga ttcaatacca atttgttgct cggcgagaaa atccatcagc
    22741 gcccgatctt caatggcttt tacgccatgt ccaatacgct ccgcacccag ttcacgaatc
    22801 gcctgccaga tgctttccgg cccggcagct tcgcctgcat ggacggtaat atgccagccc
    22861 gcatcacgcg cgcggttgaa gtgagaaagg aacagacttc ccgggaaacc aagttcatca
    22921 ccggctaaat caagtgcggt aatctggtca cggtgggcta aaaaggcctc cagctcttgc
    22981 tgacaggcgg cttcgccgaa ggtccggctc ataatgccga taagcttcgc ctgcacacca
    23041 aaggtgcggc aaccctcacg tacgccatcg atcaccgctt cgacaacacc cgctacaggc
    23101 agctgatgtg ccattgccat gtagcctggt gaaaaacgca gctcgacata gtgcaggccg
    23161 tgacgggctg catcttcaat gttttcaaat gccacgcggc gacaggcatc aagagaggcg
    23221 agaactttaa cgccccagtc aagtttggtc agaaagctca ccagatcggg ttcgttggca
    23281 atgacctgaa cgtggggaat cagtgtttcc agggattgtg caggaagcga gatattatac
    23341 tggcggccaa gttcaagaat ggtctgggga cgaatgttgc catcaaggtg gcgatggata
    23401 tcagttaatg gcagggtggt atcaatcatg gtcgcactct ttttctggat ataaaagtgc
    23461 gcaccattat aaaaacaaag cagggtaaaa atccacgatt gcgcaacgat tttttacgtt
    23521 gcgcaattgg ggttaacgaa cagcgcggat ggcgttaatt aatccagcaa cacctttttc
    23581 cagtttcgaa cgcgggcacc cggcattgag acggacaaaa ccacgacctt cttcaccgta
    23641 ggtatacccc ggcatgatcg cgaccttctc ttgctcgata agcgcttttt gcaacgcgtt
    23701 gtcgtcaata ttcaacggac gtagatcaat ccaggccaga taagtggatt gcgggatctg
    23761 ccagttgagt tcagggagcg cggagttcat tttatctgcg atatacgtca ggttatcttt
    23821 cagatagacg cgtaaggcat ccagccacgg cgcgccttgc tgataggcgg cgatatgggc
    23881 agttaacgcc agtactgaag gggaagaaag gccatcacgt cctttcagtg ccgataaata
    23941 ggcatcacgg ctgctgctat tttctataat cccataagca ccggtcagcg cgggaatatt
    24001 gaaacttttc gatcccgacg tcagcaacgc ccagtctccc cgcgccacat tactccaggg
    24061 aatatgcggc tgctcgcccc agaccatatc catatggatt tcatcggaaa taacccgcac
    24121 accatgacgc tcgcacaggt cagccatgat ctccagttca tcgcacgtcc acactttccc
    24181 ggtaggattc tgtgggctac acaggagcat aattttacat tctggtttcg ccaacacggc
    24241 ttccagcttg cccatatcgc aaaaccagcc gtcagcctgc ttctctaaag cgacgggcat
    24301 tactgtgcgc tggttacctt caatggcctt gtaaaatgcg tcataggcgg gtgtgtggat
    24361 caccacgcct tcaccttttt cagaccactg acgaatcagt tccgagacca tgtagataac
    24421 agaagggcca tacaccaccg actgcgtatc aattgcggtg taatgctggg tggaaaacca
    24481 gtgggcaata gccgcgagaa actcatcgtt tttccagcgg ctgtagccaa acacgccgtg
    24541 catcaggcgc tgattcagcg cctcgataat gcagggggca gtggcaaaat ccatgtctga
    24601 aatcgtgaac ggtaacaggt cagcagtgcc gaaacggtca gcgacataat cccactgcgt
    24661 acaccatgtg ccatgacgat ccacgacctt tgaaaaatcg aacatatctt atccttatgc
    24721 ctggacagta tgcatcagac cggccatttc atcttttact gactgaactt gtggcccgat
    24781 aacaacctgc aggttatgtt gattaagttg tactacgcca attgcccgat tgtccttcag
    24841 tgcctgcaca ttaacaagcg acatatcttt cacagacaaa cgcagacggg taatgcagtt
    24901 atcgaggctg acaatattgt cggcaccgcc tagggcttcg aggattgctg gaacgttgta
    24961 acctgattta cccggcgcac cggcaacggc tttttcgatt gagctggcaa cttcgctatc
    25021 gcgccccggg gttttcagat tgaagcgggt gatagcgaaa cggaagatga cgtagtaaac
    25081 gacaaaccag attgccgcca ccactggcac catgtaccac ttggttgaca gaccatgcaa
    25141 aataccgaac accacgaagt cgatgatatt gccgtcggta ttaccgatgg tgacgccgag
    25201 cacagacatg acggtgaagc cgaggccggt taacagcgcg tggatgacat acagaactgg
    25261 cgctacgaac aggaacagga attccagcgg ttcggtcgtg ccgccaacga cgcaggcgat
    25321 caggccagaa atcagcagac ctttaatttt atggcgattt tccgggcgcg cgcagtgata
    25381 catagctaac gctgcacctg gcagaccgcc gagaaacgca ggcattttac cttgcgaaag
    25441 gaaacgcgtg gcgctttcag aaaaaccgtg agtggtcggg caactcaatt gcgcctggaa
    25501 gatggtcagt gcgccgctga cggtttgacc gcagacttcc tgcgtgccgc ctgcgtcggt
    25561 aaagcgaatt aatgccacca gaatgtgatg cagaccaaac ggcaacagca gacgttcacc
    25621 ggtaccaaac agcatcggtc cgaaatcacc cgcactgttg atcatatggc ccaggccgct
    25681 aatacccatg gcgaaaatcg gccagactaa tggaatcacc agaccgacaa ggcccatcac
    25741 cagcgaggag atgattggta cgaagcgcgt accgccgaag aatgccagcg catccggcag
    25801 gcggatatta tggaatcgct catgcagcat ccagacgata ataccggcga tcaccgcacc
    25861 gaggatcccg gtatcgatcg actggatccc aagaatgctc tgtatgttat tggctttcag
    25921 aacggcggcg tctgtggttg gcagaatgcc tttattggtt aaccagaagt ttaccgcgag
    25981 gttcattacc gcataaccga cgaagccagc gaatgccgct acgcctttgt tttcgcgtgc
    26041 caggcccagc gggatggcga tacagaacat gactggcagg aaactaaaag caaacgagcc
    26101 aatcttactc atccaggtaa agatagcctg caacacgggg ttgcccagga ccgggatcag
    26161 ggttatgaca tcatggctgc taagagaact accaatgccg agcataatgc cgcagaacga
    26221 caataatgcc acgggtaaca tgaaggtttt gcctaactgc tggaagaact cccacagcgt
    26281 gactttcggt gctgttttcg ccgtcataaa acgactcctc gtagagaaca atctggattc
    26341 agtaaattgc gcgagaagat aaaacgtttt atcaaatttt agtgaggcat aaatcacatt
    26401 acgcaacgat aatagcgggt ataagataaa taaaaggtaa aacgttttat ctgtcacata
    26461 atcagggagt aggtcatctg catggctacc gccaaaaaaa taaccattca tgatgttgcg
    26521 ctggctgcgg gcgtgtcggt aagtaccgtt tcgctggtgc taagtggcaa agggcgaatc
    26581 tctaccgcca caggagaacg cgttaacgcc gccattgaag agctgggatt tgtgcgcaat
    26641 cgtcaggcgt cggcgctgcg cggcgggcaa agcggcgtca ttggtttgat cgtccgtgat
    26701 ttatctgcgc cgttttacgc cgaattaacg gccggattga cggaagctct ggaagcgcag
    26761 ggacggatgg tttttttgct tcacggcggt aaaaacggcg agcagctggc acagcggttt
    26821 tcactgttac tgaatcaggg tgtcgatggt gtggtaattg ccggggctgc aggaagcagc
    26881 gatgacctgc gacggatggc agaagaaaaa gctatcccgg ttattttcgc ttcccgtgcc
    26941 agttatcttg atgatgttga tacggttcgc ccggacaaca tgcaggctgc acagttgttg
    27001 acggagcatc tcattcgcaa tgggcatcag cgtatcgcct ggctgggagg gcaaagttcc
    27061 tcattaaccc gggcagaacg ggtggggggc tattgtgcaa ctctactaaa atttggcctg
    27121 ccgtttcaca gtgattgggt gctggagtgc acttccagcc agaagcaagc cgcggaagct
    27181 atcacggcgc ttttacgtca taacccgacc atcagtgccg tggtttgcta taacgaaacc
    27241 attgcgatgg gggcatggtt tggtttgctg aaagcagggc ggcaaagcgg tgaaagcgga
    27301 gtcgatcgtt actttgaaca acaggtttcg ctggcggcat ttaccgatgc gacaccaacc
    27361 acacttgatg atagacctgt tacctgggcc agcacgccag cgcgggaact tggtaccaca
    27421 cttgcggatc gcatgatgca aaaaatcacc catgaagaga cgcattcacg caatcttatt
    27481 attcccgccc ggctcattgc ggcgaaataa tcctctcttt atctgctata cctggtagtg
    27541 tcctttcctc aaggttaatg ataaaaaatg tggcacacag gcggtgcctt ttatttttac
    27601 tgaagttcac aggaggttta tgtgtttaat tctgacaacc tgagactcga cggaaaatgt
    27661 gccatcatca caggtgccgg tgcaggtatt ggtaaagaaa ttgccattac attcgcgaca
    27721 gctggcgcat ctgtggtggt cagtgatatt aacgccgacg cagctaacca tgttgttgat
    27781 gaaattcaac aactgggtgg tcaggcattt gcctgccgtt gtgatattac ttccgaacag
    27841 gaactctctg cactggcaga ctttgccgtc agtaagctgg gtaaagttga tatcctggtt
    27901 aacaacgccg gtggcggtgg tcctaaaccg tttgatatgc caatggcaga ttttcgccgt
    27961 gcttatgaac tgaatgtgtt ttcttttttc catctgtcac aacttgttgc gccagaaatg
    28021 gaaaaaaatg gcggtggcgt tattctgacc atcacttcta tggcggcaga aaataaaaat
    28081 ataaacatga cctcctatgc atcatctaaa gctgcggcca gtcatctggt cagaaatatg
    28141 gcgtttgacc tgggtgaaaa aaatattcgg gtgaatggca ttgcgccggg ggcaatatta
    28201 accgatgccc tgaaatccgt tattacacca gaaattgaac aaaaaatgtt acagcacacg
    28261 ccgatcagac gtctgggcca accgcaagat attgctaacg cggcgctgtt cctttgctcg
    28321 cctgcagcca gctgggtaag cggacaaatt ctcaccgtct ccggtggtgg ggtacaggag
    28381 ctgaattaat acactaacgg accggtaaac agccgtacgt gttgtttacc gggatagcgt
    28441 gtgcaattca ttaacgtctc tgcgaaacaa ctggcagcca aatcacggct attggttaac
    28501 caatttctgg gtgaaaagta tacgaataga gtgtgccttc gcactattca acagcaatga
    28561 taggcgctca cctgacaacg cggtaaacta gttattcacg ctaactataa tggttttatg
    28621 atggataaca tgcagactga agcacaaccg acacggaccc ggatcctcaa tgctgccaga
    28681 gagatttttt cagaaaatgg atttcacagt gcctcgatga aagccatctg taaatcttgc
    28741 gccattagtc ccgggacgct ctatcaccat ttcatctcca aagaagccct gattcaggcg
    28801 attatcttac aggaccagga gagggcgctg gcccgtttcc gggaaccgat tgaagggatt
    28861 catttcgttg actatatggt cgagtctatt gtctctctca cccatgaagc ctttggacaa
    28921 cgggcgctgg tggtcgaaat tatggcggaa gggatgcgta acccacaggt cgccgccatg
    28981 cttaaaaata agcatatgac gatcacggaa tttgttgccc agcggatgcg tgatgcccag
    29041 caaaaaggcg agataagccc agacatcaac acggcaatga cttcacgttt actgctggat
    29101 ctgacctacg gtgtactggc cgatatcgaa gcggaagatc tggcgcgtga agcgtcgttt
    29161 gctcagggat tacgcgcgat gattggcggt atcttaaccg catcctgatc ctctctctct
    29221 ttccgcgggc tggtgacaac tgagcccgcg tttcatatcg taatttctct gtgcaaaaat
    29281 tatccttccc gggttcggag aattcccccc aaaatattca ctgtagccat atgtcatgag
    29341 agtttatcgt tcccaatatg ctcgaacgaa cgttcggttg cttattttat ggcttctgtc
    29401 aacgccgttt taaagattaa tgcgatctat atcacgctgt gggtattgca gtttttggtt
    29461 ttttgatcgc ggtgtcagtt ctttttattt ccatttctct tccatgggtt tctcacagat
    29521 aactgtgtgc aacacagaat tggttaacta atcagattaa aggttgacca gtatttttat
    29581 cttaatgagg agtcccttat gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa
    29641 ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga tcagcgttgg
    29701 tgggaaagcg tgttacaaga aagccgggca attgctgtgc caggcagttt taacgatcag
    29761 ttcgccgatg cagatattcg taattatgtg ggcaacgtct ggtatcagcg cgaagtcttt
    29821 ataccgaaag gttgggcggg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac
    29881 ggcaaagtgt gggtaaataa tcaggaagtg atggagcatc agggcggcta tacgccattt
    29941 gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg
    30001 aacaatgaac tgaactggca gactatcccg ccgggaatgg tgattaccga tgaaaacggc
    30061 aagaaaaagc agtcttactt ccatgatttc tttaactatg ccgggatcca tcgcagcgta
    30121 atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg
    30181 caagactgta accacgcgtc tgttgactgg caggtggtag caaatggtga tgtcagcgtt
    30241 gaactgcgtg atgcggatca gcaggtggtt gcaactggac aaggtaccag cgggactttg
    30301 caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga actatgcgtc
    30361 acagccaaaa gccagacaga gtgtgatttc tacccgctgc gcgtcggcat ccggtcagta
    30421 gcagtgaagg gcgaacagtt cctgatcaac cacaaaccgt tctactttac tggctttggt
    30481 cgtcatgaag atgcggattt gcgcggcaaa ggattcgata acgtgttgat ggtgcacgat
    30541 cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta cccttatgcc
    30601 gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga aacagcagct
    30661 gtcggcttta acctctcttt aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg
    30721 tacagcgaag aggcagtcaa cggggaaacc cagcaggcgc acttacaggc gattaaagag
    30781 ctgattgcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc caacgaaccg
    30841 gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc aacgcgtaaa
    30901 ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat
    30961 accatcagcg atctttttga tgtgctgtgc ctgaaccgtt attacggttg gtatgtccaa
    31021 agcggcgatt tggaaacggc agagaaggta ctggaaaaag aactgctggc ctggcaggag
    31081 aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc cgggctgcac
    31141 tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac
    31201 cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt
    31261 gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aggggatctt cacccgcgac
    31321 cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat gaacttcggt
    31381 gaaaaaccgc agcagggagg caaacaatga atcaacaact ctcctggcgc accatcgtcg
    31441 gctacagcct cggtgacgtc gccaataact tcgccttcgc aatgggggcg ctcttcctgt
    31501 tgagttacta caccgacgtc gctggcgtcg gtgccgctgc ggcgggcacc atgctgttac
    31561 tggtgcgggt attcgatgcc ttcgccgacg tctttgccgg acgagtggtg gacagtgtga
    31621 atacccgctg gggaaaattc cgcccgtttt tactcttcgg tactgcgccg ttaatgatct
    31681 tcagcgtgct ggtattctgg gtaccgaccg actggagcca tagcagcaaa gtagtgtatg
    31741 catatttgac ctacatgggc ctcggacttt gctacagcct ggtgaatatt ccttatggtt
    31801 cacttgctac cgcgatgacc caacaaccac aatcccgcgc ccgtctgggc gcggctcgcg
    31861 ggattgccgc ttcattgacc tttgtctgcc tggcatttct gataggaccg agcattaaga
    31921 actccagccc ggaagagatg gtgtcggtgt accatttctg gacgattgtg ctggcgattg
    31981 ccggaatggt gctttacttc atctgcttca aatcgacgcg tgaaaatgtg gtacgtatcg
    32041 tggcgcagcc gtcattgaag atcagtctgc aaaccctgaa acggaatcgc ccgctgttta
    32101 tgttgtgcat cggtgcgctg tgtgtgctga tttcgacctt cgcggtcagc gcctcgtcgt
    32161 tgttctacgt gcgctatgtg ttaaatgata ccgggctgtt cactgtgctg gtactggtgc
    32221 aaaacctggt cggtactgtg gcatcggcac cgctggtgcc ggggatggtc gcgaggatcg
    32281 gtaaaaagaa taccttcctg attgacgctt tgctgggaac ctgcggttat ctgctgttct
    32341 tctgggtttc ggtctggtca ctgccggtgg cgttggttgc gttggccatc gcttcaattg
    32401 gtcagggcgt taccatgacc gtgatgtggg cactggaagc tgatactgta gaatacggtg
    32461 aatacctgac tggcgtgcga atagaagggc tcacctattc actattctca tttacccgta
    32521 aatgcggtca ggcaatcgga ggttcaattc ctgcctttat tttgggatta agcggatata
    32581 ttgccaatca ggtgcaaacg ccggaagtta ttatgggcat ccgcacatca attgccttag
    32641 taccttgtgg atttatgctg ctggcattcg tcattatctg gttttatccg ctcacggata
    32701 aaaaattcaa agaaatcgtg gttgaaattg ataatcgtaa aaaaatgcag cagcaattaa
    32761 tcagcgatat cactaattaa tattcaataa aaataatcag aacatcaaag gtgcaactat
    32821 gagaaaaata gtggccatgg ccgttatttg cctgacggct gcctctggcc ttacctctgc
    32881 ttacgcggcg caactgactg acgatgaagc gggactacgc atcagactga aaaatgaatt
    32941 acgcagggct gataagccca gtgctggcgc gggaagagat atttacgcat gggtacaggg
    33001 aggattgctc gatttcaata gtggttatta ttccaatatt attggcgttg aaggcggggc
    33061 gtattatgtt tataaattag gtgctcgtgc tgatatgagt acccggtggt atcttgatgg
    33121 tgataaaagt tttggctttg ccctgggagc agtaaaaata aaacccagtg aaaatagcct
    33181 acttaaatta ggtcgcttcg ggacggatta tagttatggt agcttacctt atcgtattcc
    33241 gttaatggct ggcagttcgc aacgtacatt accgacagtt tctgaaggag cattaggtta
    33301 ttgggcttta acgccaaata ttgatctgtg gggaatgtgg cgttcacgag tatttttatg
    33361 gaccgattca acaaccggta ttcgtgatga aggggtgtat aacagccaga cgggaaaata
    33421 cgataaacac cgcgcacgtt cttttttagc cgccagttgg catgatgata ccagtcgcta
    33481 ttctctgggg gcatcggtac agaaagatgt atccaatcag atacaaagta ttctcgagaa
    33541 aagcataccg cttgacccga attatacgtt gaaaggggag ttgctcggct tttacgcgca
    33601 actcgaaggt ttaagtcgta ataccagcca gcccaatgaa acggcgttgg ttagtggaca
    33661 attgacctgg aatgcgccgt ggggaagcgt atttggcagt ggtggttatt tgcgccatgc
    33721 aatgaatggt gccgtggtgg ataccgacat tggctatccc ttttcattaa gtcttgatcg
    33781 taaccgtgaa ggaatgcagt cctggcaatt gggtgtcaac tatcgtttaa ctccgcaatt
    33841 tacgctgaca tttgcaccga ttgtgactcg cggctatgaa tccagtaaac gagatgtgcg
    33901 gattgaaggc gcgggtatct taggtggtat gaactatcgg gtcagcgaag ggccgttaca
    33961 agggatgaat ttctttcttg ctgccgataa agggcgggaa aaacgcgatg gcagtgcgct
    34021 gggcgatcgc ctgaattact gggatgtgaa aatgagtatt cagtatgact ttatgctgaa
    34081 gtaagaaaaa aatgccggag agaaaaatct ccggcgtttt ggctgtgaat tactgctgcg
    34141 gaatagcggg tacagccgga acgtttaatg ccggcattgc aaacatccca acaaaatctt
    34201 ccagtggcat tttctgcccg tttaacgtga tctgaccgtt agcatattgc aggctggtgg
    34261 tgatggtatt gtcctgcaag gtggtcagac ggaacatctg ccccattgct gatgcacctt
    34321 caacttgctg tttcgccagt tttttcgctt gatcttcctg ataaccttcc agcttcgcta
    34381 cctgagtcat aaactcagtt gccatatcca ccggaatggt cagtttcgca tccagagatt
    34441 taaccgaacg atctacttcc tgcgccagcg tttgcggcgc ttctttagtc gttgccggat
    34501 ctttcaggaa cagcgacaga ttcagcgcac tttcaccctg actgtttttc cagcttagcg
    34561 gcgcgatagt aatcaccgga tcgcctttca acatcagcgg cagggcgcta aagaaggctt
    34621 ccgtcacttt ctcctgataa agttcgggat tgttggcaat ttctggctgt gccagcagcg
    34681 cctgagtttg cgcgttatat tgctggctaa actgatgcca ggcttcacca tcaatttggc
    34741 cgacttttag agtcagcttg ccactgccca gatcctgatt ctgtaccttc aggctgttta
    34801 gcgagtaatc cagttggcta ttgatcgttt taccgtcatt gaccagatcc gatttaccgc
    34861 tgatctccat gccttgcagc agtgccagtt ctttgccttc cactgaaatg gtcatttttt
    34921 ccagtgacag tttttggttt cctacgcgct caccaaaact tgccagcgtg ctggaaccgt
    34981 cggttttcag attattaaag gtcaactgca ctttctggtt gtattcgtta actgcgtcta
    35041 tccgaccact ttgcgcctcc ccggaaaggg agatagcttt gccgtctctg tctgcattta
    35101 actggaactc gccgccgcta aaggcgactt tttcatcctt ttgctcgtaa ttcagtggct
    35161 tgagcgaaat atcggaactg gaatctccgc tgtaaccaat gcgggagtta atctcaaaag
    35221 gcgtttcacc ttttgccata tcaaacagtg gcttgctaac ttcgttatta accagcgtgg
    35281 tttgaattga tgccatcgac gggatcaggt tcagtttttt tagctgggca agggggaagg
    35341 gaccatgatc aaccgattcg ttgaagatga cgctctgacc gcttttaatc cacggatttt
    35401 ctttcccggc aatgggtttc accaacagtt gcagctgact gctgaatacg ccgcgatgat
    35461 agttttgata actcacttcc aggttggatt taggagcggt cagtttgagt tgcgcgttcg
    35521 cctgcgcgac catgtcttcg agatgggttt caatcttctt gcctgtatac catgcgccgc
    35581 ctgtccagac tacgcctagc gcaacaatga cgcctaccgc taccagcgat ttattcataa
    35641 tgattatcca taaaatgaaa tcaggcggac tggccgcctg aaggtgttat aagcctttaa
    35701 taagcttagc aagagatgtt aattttttca gtaagctctt acagcttgtt gtaaacacgc
    35761 gctaaacggc cgtggccttt gacagtcacc ggtgattcgt tggcggcaat aaacgctgat
    35821 tcacccggtt taagctgtaa ctgctgagaa cctttacaca gcgttgcatc gccttcgacg
    35881 cagaacaaaa tggcggcact ctgctggctg atggtggttt ctttatcact aaggtcatgc
    35941 agcgagaagg caaaatcatc aacaggaatc gggaagtcca gttctgcacc ttgtttcacc
    36001 ggctgggtca acaactggtt agccggtttg gcttcgaact tcacattggc aaccagttcc
    36061 ggaatatcaa tgtatttagg cgtcagaccc gcacgcagca cgttatcgga gttagccatc
    36121 acttccagcg ccacgccttg caggtaagcg tgcggtgttt cagcgaacag gaacatcgct
    36181 tcgccagggt tcaatttcac cacattcagc aatagcgggg agaacagacc gctgtcttcc
    36241 gggtaaaatt cagaaattaa acgaatcgtt tgccacggtt caccctgctg gctgtcgagc
    36301 gccgatttta aaatcgccag cgcgcgggat ttttcttccc cctgcatatt cagcaggctg
    36361 gcgaacagtt cgcttaaacg ttcggcatca ggctgttgta aaaagtgagc aatcgccgga
    36421 tgtgcacctg cgactggctg gagtagggaa acaatctcgg aaaattcacg aaacgcgttc
    36481 atcgcaagga aaggcgtcag cgcaaaaacc agctccggct tgtggttagg atctttatag
    36541 ttacgctcgg cggcatccat cggaatacct gcggcatttt ctttggcaaa accgatttca
    36601 gaattgcgtt tgtttggatg aacctgaatg gagagcggct gtgctgcgca taatactttg
    36661 aacaggaaag gcagttcgcc aaagcgtttg gcaacggcat ctcccaaaag agtcgcttta
    36721 tcactctcaa tcacatcacg cagtgaaacg atatctccgg cggcattctg cacccgtgaa
    36781 ctgcttttcg gatgtgcgcc catccacagc tcggccatcg gctggctgga cggattttcc
    36841 ataccataaa gttcagtcaa cgccgttttg ctgccccagg catagttttg cactgagtta
    36901 atgagttttt gcatgatcaa tccctgtttt aatgtggaaa ttaatcccgc tattaaagca
    36961 agaatcctac gggaagtaac ctggagccgc aaaaagtcgt actagtctca gtttttgtta
    37021 aaaaagtgtg taggatattg ttactcgctt ttaacagggc aacggaacac ccgcccagag
    37081 cataaccaaa ccaggcagta agtgagagaa caatgtcaaa caaacccttt cattatcagg
    37141 ctccttttcc actcaaaaaa gatgatactg agtattacct gctaaccagc gaacacgtta
    37201 gcgtatctga atttgaaggg caggagattt tgaaagtcgc acccgaagcg ttaactttgt
    37261 tggcgcgtca ggcgtttcat gatgcgtcat ttatgctgcg tccggctcac caacaacagg
    37321 tggccgacat tctgcgtgac ccggaagcca gcgaaaatga taaatatgtg gcgctgcaat
    37381 tcctgcgtaa ctccgacatc gcggcgaaag gcgttctgcc aacctgtcag gataccggca
    37441 ccgcgattat tgttggtaaa aaagggcagc gtgtatggac cggtggcggt gatgaagcgg
    37501 cgctggcacg cggtgtctat aacacttata tcgaagataa tctgcgctac tcgcaaaacg
    37561 cgccgctgga tatgtataaa gaggtgaata ccggcaccaa tctaccagcg cagatcgatc
    37621 tttatgccgt tgatggcgac gagtacaaat tcctctgtat cgccaaaggt ggcggttcgg
    37681 caaacaagac gtatctctat caggaaacca aagcgttact aacgccgggg aaactgaaaa
    37741 attacctggt tgagaagatg cgcacgctgg gtacggcggc ctgtcctccg tatcatattg
    37801 cgttcgttat tggtggaact tctgcagaaa cgaaccttaa aacggtgaaa ctggcttccg
    37861 ctaaatacta tgatgaactg ccaacggaag ggaatgagca cggacaggcg ttccgcgatg
    37921 tggaactgga aaaagaattg ctgatcgaag cgcaaaatct tggtctgggt gcgcagtttg
    37981 gcggtaaata cttcgctcac gacatccgcg tgattcgcct gccacgtcac ggcgcatcct
    38041 gcccggtcgg tatgggcgtc tcctgttctg ctgaccgtaa tatcaaagcg aagatcaacc
    38101 gtcaggggat ctggatcgaa aaactggaac ataatccagg aaaatatatc ccggaagagc
    38161 tgcgcaaagc gggagaaggc gaagcggtgc gcgttgacct taaccgtccg atgaaagaga
    38221 tcctcgcaca gttgtcgcag tatcccgttt ctacacgctt atcgctgaat ggcacgatta
    38281 tcgtcggtcg tgatattgct cacgccaaac tgaaagagcg gatggataac ggtgaagggc
    38341 tgccgcagta catcaaagat catccgattt actatgcggg tccggccaaa acgccggaag
    38401 gctatgcctc cggttctctt ggcccaacga ccgccggacg gatggattct tatgtcgatc
    38461 aactgcaagc gcagggagga agtatgatca tgctggcgaa aggcaaccgc agccagcagg
    38521 tgacggatgc ctgtaaaaaa cacggcggtt tctacctcgg tagtatcggt ggtccggcag
    38581 cagtattggc gcagggaagt attaagagcc tggaatgtgt tgaatatccg gaactgggaa
    38641 tggaagccat ctggaaaatt gaagtggaag atttcccggc gtttatcctt gtggatgata
    38701 aaggaaatga cttcttccag cagatacaac tcacacagtg cacccgctgt gtgaaataaa
    38761 cagagccgcc cttcggggcg gtttttttac atggcacgaa agaccaaata tttgttatca
    38821 aatagtaaat aatgagtgag ctaaaagttg cttaacgaaa gcaaaacaga aagaaaaaat
    38881 taatcaggtg aggagcaggc catgaataca gtacgcagcg aaaaagattc gatgggggcg
    38941 attgatgtcc cggcagataa gctgtggggc gcacaaactc aacgctcgct ggagcatttc
    39001 cgcatttcga cggagaaaat gcccacctca ctgattcatg cgctggcgct aaccaagcgc
    39061 gcagcggcaa aagttaatga agatttaggc ttgttgtctg aagagaaagc gagcgccatt
    39121 cgtcaggcgg cggatgaagt actggcagga cagcatgacg acgaattccc gctggctatc
    39181 tggcagaccg gctccggcac gcaaagtaat atgaacatga acgaagtgct ggctaaccgg
    39241 gccagtgaat tactcggcgg cgtgcgtggg atggagcgta aagttcaccc taacgacgac
    39301 gtgaacaaaa gccaaagttc taacgatgtc tttccaacgg cgatgcacgt tgcggcgctg
    39361 ctggcgctgc gcaagcaact cattccgcag cttaaaaccc tgacacagac actgaatgag
    39421 aaatcgcgcg catttgccga tatcgtcaaa atcggtcgaa cccacttgca ggatgccacg
    39481 ccgctaacac tggggcagga gatttccggc tgggtagcga tgctcgagca taatctcaaa
    39541 catatcgaat acagcctgcc gcatgtagcg gaactggctc ttggcggtac agcggtgggt
    39601 actggactaa atacccatcc ggagtatgcg cgtcgagtag cagatgaact ggcagtcatt
    39661 acctgcgctc cgtttgttac cgcgccgaac aaatttgaag cgctggcgac ctgtgatgcc
    39721 ctggttcagg cgcacggcgc gttgaaaggg ttggctgcgt cactgatgaa aattgccaat
    39781 gatgtccgct ggctggcctc tggcccgcgc tgtggaattg gtgaaatcgc aatcccggaa
    39841 aatgagccgg gcagctcaat catgccgggg aaagtgaacc caacccagtg tgaggcatta
    39901 accatgcttt gctgtcaggt gatggggaac gacgtggcga tcaacatggg tggcgcttcc
    39961 ggtaactttg aactgaacgt cttccgtccg atggtgattc ataatttcct gcaatcggtg
    40021 cgcttgctgg cagatggcat ggaaagtttc aacaaacact gtgcagtggg cattgaaccg
    40081 aatcgtgagc gaatcaatca attactcaat gaatcgctga tgctggtgac tgcgcttaac
    40141 acccacatag gttatgacaa agccgccgag atcgccaaaa aagcgcataa agaagggctg
    40201 accttaaaag ctgcagccct tgcgctgggg tatcttagcg aagccgagtt tgacagctgg
    40261 gtacggccag aacagatggt cggcagtatg aaagccgggc gttaatctgc aacatacagg
    40321 tgcagccgtg gaatgatcaa acgaagcggc tgcgcctgtg gtttataacg gtgttgcaca
    40381 ttgtcggcat cgtaatttaa caactcccca acgtccggca cgcccgcgcc attatcccga
    40441 ttaatcagcg caataagcgg tgtagggcaa gcgtgttgta cttgtttttg atctcccttg
    40501 taccagacgc gggcaatcgg ctgcaccttc accggacgtt tgatttttaa cttcgcgtcc
    40561 tgtggcaggg tagcaatatc ctgatactct ctctccagtt ttctttgcca ctcctcgcgc
    40621 gtccacggtg cgacactgcg cggtgatttc aggctttttt ccagctgtgc caggacttca
    40681 tcacgatgca aattcttaat gatatgttta ttggcccacc caaaacgtaa agtggcgggg
    40741 tcgtgcagaa cagtgagcgt tcggtaagca ttaagggtga tcagccccgg caaatgacga
    40801 tgcacccatt caaaacgcgc cgctgtgggg agttctgact caaccgtgac gatatgctcg
    40861 aacgtagtct tgagttgatt gatgtgctga atatgactga ccaacgctgc ttgcgaaagg
    40921 ttatcgacct ggtaacacaa cacgccaggt agacgaacag cggccttgct gctgcgattt
    40981 tcggactgtt gttgaataaa taaatggcgg aaatgacgta acgccagcga ctgcgcgtcg
    41041 ttgccgagat gttgctttac ctcaatacga ttaagcggat tatgctcatc ctcttttttt
    41101 acctccggca aagagaacac gcgggcaacc agcaatttgt gctgttctag ctcagcggca
    41161 aatgcaccca gttcttgttc catctggcga aaggtagtgt tgagtcggtc tacgagatcg
    41221 taacgcgcca taatattaac cactttagtt acaacatact ttattttata gcacagtggt
    41281 ggtgacttga ccaccgtgcg cgtagtgtca ggcagaggta aattgcggga tgttatgcca
    41341 taacggccag ctaaacgaga agcgggcacc acccagttcg ctggtgtcac aattaaccgt
    41401 accgcccatt gccagtgcta tagagtggac aatcgccaac cccagcccgc agccgccggt
    41461 tgagcgatcc cggctgggat cgaggcgaac aaaaggttca aagatatgtt cgcggttttc
    41521 tggggcaatc cctgggccat catcctcaac aattaatgtc gctctattcc ccgacagtag
    41581 caggctggtt tcaaccgttg agtgacagta gtgcagggcg ttattgagca aattatccag
    41641 cacgcgctcc attaagcgca tatccaacgc cgcataatgg ccttgcgcta gcgttttaat
    41701 ccgtaccgtt ttatcgggcg ttactgcctg aatgtctgcc agatgcgttg acagccacaa
    41761 cggcaggtct ggttcgctaa gatgaagctc attttgtggg cgatcgagtc gggcataagt
    41821 cagcagctct tcaattaaag cttcaagttg actgatatcg cgattcaacg cctgggattc
    41881 ggcggcgctc aggttatcgc tcatctccag tcgataacgc aggcgcacta acggtgttcg
    41941 cagttcgtga gcgataccgt cgataagctg ttttttgctg gcaatcaagg cgttgatatt
    42001 gtccgccatc tggttaaatg cgacgccaag tcgttcaaag ctcgaacctt catcaaagtg
    42061 gatacgctca ctgagatgcc catcgccaaa tcgttgcgcc gctgcttcca gttttaacat
    42121 atcctgccag tgcggacgca tccagataaa caccggaaag gcgagggaaa tggcaataaa
    42181 agcgatcagg gcgatatcca gtaatcgcat ctgatggagg taataaagat aaggaacagg
    42241 accaactgcc agcacgtagt ggctgcgcgg gatacgttgc aaaaaagtgt actgatcgtc
    42301 cagggcgaca atttcgccac cacgcagccg gtgcatggaa atatcatcaa gatgatattt
    42361 actcagcggc tcgacacgca gatcgaaaga gagatttaaa tccatctcct tcagcgtctt
    42421 accccaatcg tgtgggggga tctcacgcaa ttcgctgcgc atcagataca gcgaactgtt
    42481 catcaaatca tccagcgact gtttgcctgc gcgttcggcg gtaaatttgt acaccagccc
    42541 gaccagcaga gacatcacaa ggaagcagac aaacaataac aggtaaaact ggataaacag
    42601 ttttttcatc gcttattccc acgcatgagg cgcaaaaaga tagcctttgt tacgcacggt
    42661 tttaatacga taaggttctg cggcgttatc gagcagtttt tttcttaacc gcgaaatagc
    42721 cacgtccacg ctacgatcca gaccgtcata actgacgccg cgtaaatttt tcagcaatgc
    42781 atcgcggtcc atgatttgcc cggcatgggt agctaattcc cacaataatt cgaaatcagc
    42841 tgtcgagagc gagatttcag tgttagccag ggtgactacg cggttgatgg gatcgatggt
    42901 caacgtgccg aaatgcaggg ctttgtaggg agtcagagac gtttcctgaa tacctttggt
    42961 cactgtagct tgctcattct gacgcaaatg caaacgtaaa cgcgctagca aaacagcagg
    43021 gggcgtcgtt ttgagaatat agtcgcaggc gcccatttcc agtgccagga tgtggttcat
    43081 atcgctatcg agagaggtta gaagaacaat cggtccggac cactttgcgc gtaaatcacg
    43141 acaaatggtc atgccgtcct tgcctggtag catgatgtcg agtaacacca aatccggatt
    43201 ttctcgcaga atagtttctt cggcccggtc gccgcgcggc tctacggtaa cctgcatatc
    43261 atgttttgcc aggtacgcag caatcagtgc accgacttcc gcatcatctt ccacaaatac
    43321 gatagtgttc ataacattca cggtagatat aaaaacgtca caatacaccg cactgttttt
    43381 acttactatc aatcgtttct aaacaaggtt tcatcaggta ttctactgat gacttgttat
    43441 ggaagtgtta aggtaaaaag atggggctgg taatcaaagc cgcgctcggt gcgctggtag
    43501 tgctgttgat tggtgtttta gcaaaaacga aaaattatta tatcgccggg ctgataccac
    43561 ttttcccgac ctttgcgctt atcgcgcatt atattgttgc cagcgaacgc ggcattgaag
    43621 ccttacgcgc aaccatcatt tttagtatgt ggtcgattat tccctatttt gtttacctgg
    43681 cgtcgctgtg gtatttcacc ggaatgatgc gcttgcccgc cgcttttgtt ggttctgttg
    43741 cgtgttgggg gataagcgca tgggtgttga ttatatgctg gataaagctg cattaacgca
    43801 gatgacgacc gccatcaaga gggaaactgc gtccggtgac aaagcagctg gtaagtaagt
    43861 aatcgaccag gtcgatcact tctttctcgc caggcgcggt tttcatcagt gatttattca
    43921 gcgcctgttg tcgatattcg gcatcatcat gttcattaaa caggatcagc gatggcgcaa
    43981 tagaattcac tttcacttcc ggtgccagct tgcgggcaaa cgagcgagtc atattatcca
    44041 gtgccgcttt gcttgcagca taggcaatat gtttgtcgct accgcgctcc accacataat
    44101 cggtaaagtg aatgatatcg ctggcggcgt gtccgtgccc acgcagtaat ctttccagtg
    44161 catggttgag caggtatggg gtattaacgt ggatctgcat catgcaagcc agtacgtcgg
    44221 tcagtggcgc tcccggtttt tccgccatcc acgcgctggc gttatgcaaa atagcacgca
    44281 gaccatgggt gctttttagt acttcatcgg caaacgccat cacaccgtcg ttggtcgaaa
    44341 aatcagcctg aatacactgc gcacctgctt taatcagtcc atcaatggcg ggatagtgcg
    44401 tccgatagct gacaatcacc ggttgctttt gattaatgaa atgccatgcg agggcgaggc
    44461 cgatgcggcg acctccgcca gtaattaata ttggcaaggg ctgggtttta cccatcgtga
    44521 tctcctttgc tatccaacga tgggatgaac gttatcccac cagcatccag gtcgccggaa
    44581 ctgaggcaat taacagcatg ccaatcagca ccatttcctg acgattcagt acattatcat
    44641 gggtatgcgt tttacgcgcg tacaggaaaa ccagcagccc cggcgcatac agaacaacgg
    44701 ataacagcaa gtgcattgga ccagaggcgt acagtaacca taagccataa atgcaggcac
    44761 cgacacctac cgccttatgc agtggacggg ttgcgatttt cagcaagaac gcgccgacca
    44821 ggaaataggg caccagaatc atttctgagg cgatggtcag caacgtgttg taatcggaac
    44881 cggtcagcca gatgagtacc agacaaattt gcacgcagat gttggtcaac cacagtgagg
    44941 cggatggcgc agcttgtgcg ttctggcgcg caaaaatgcg cgggaatgct ttatgggtgg
    45001 ctgcaaggaa cggaacttct gctgccatga tggtccagct taagtacgcg ccgcaaacgg
    45061 aaacgatcaa accggctgcg atgatgattt cgccccatgg tcccatcatt tccaccatca
    45121 gaccggccat tgacgggtta cgaatttcag ccagttcagg acgtgccacc acacccagcg
    45181 aaagcagcgt taccagcaag taaacgccca gagcggagag aaccgccagc agtgtcgctt
    45241 tgccaacatc acgtttatta cgcgcacgcg cagaaacaac cacggcacct tccacaccaa
    45301 tgaaaaccca cagggtgatc agcatggtgt ttttcacctg ttcccaaacg ggtacgccaa
    45361 gtgcaagtcc ggtgaagtcg agcttgaagg tatccagttt gaacatcatc atcgccagca
    45421 caacaaacag acccagcggc aacaattttg ccagtgtcgc caccaggtta atgctggcag
    45481 cggtttgcac cccgcgcagg atcaaaaagt gaacaatcca caataaagcc gatgcaccga
    45541 ctatcgattg ccaggtatta ccatcgccaa acaggcgcaa ttccggcgtg tccgtaaaaa
    45601 agcttaacgc ggaaaaaacg atcaccagat aggagacgtt ggcgatgacc gcgcacagcc
    45661 agtatcccca tgcggaacaa aagccgatta gctcgccaaa cccttcgcgg gcataggtaa
    45721 agataccgcc gtcaagttcg ggacgaatgc gcgtgaggat cagcatggca aaggccagta
    45781 ataaaatacc agcaccagta ataccccagc cgatgagcag tgctgccggg ctggcaactg
    45841 ccgccatatt ttgcggcaga ctgaaaacac ccgcgcccag cattgagctt aataccagcg
    45901 cggtgagtgc gctcagtccc agtttctttt ccatcgatat ccagtgatta aaacgtgata
    45961 agaagaataa atattagggc aaaacgcata aaaatggagt ttgccactga agcgccggat
    46021 tttacgaaga gatgtgtctg catgcaatgg tcgcggggaa atttgtgact aaaaatgcga
    46081 aaggcggcat caagccgcct tataggagta actgaatagt tatttataca gatctgcgct
    46141 gacggtcagg ttattaccac gttcctgcca ctggcgggtg atgtggtaat acttagcacc
    46201 tttcttcgcg gcacgtttcg caacctgata ggagacttcg gtcatgttgc cgtagttgcc
    46261 agagaatttg atgctgtcga acgggaccat catcgctgcg gtcgctttgt tcagttcttc
    46321 gactttagtg ccatccggga gcgtgacggt gtaacgcccg ccttttgatg actgggtttc
    46381 aaagaagcga ccgacttcag aacttggtga tgcggtagtc gcaacacccg ggatctcaac
    46441 tttcttcgca gcttcgccac cggcagccag agctgcacgt cctgcttcgg aatctgccgg
    46501 gatcacatcc gggctctgga cgatacgttt cttagcatct tttttataga tgaatgcagt
    46561 aatacgctgg ttgccgccct ggttggcatc gatttgacga acaatgtaga aagagtaggc
    46621 acctttcgct tttgctgctt tggtgatggc atcattgact tctggctggc tacgataaaa
    46681 gctctggacg gtgaccgtgt caaacggttc aatcagaaca gcctgatctt tcggcagttc
    46741 gacaacaccg ttaattacgc gattacttgt ttcttctgct ttttcagcat cggctttata
    46801 gaggtcagcg accacacgcc agttaccgct gttaccaaaa tcagaagtgt cgacaacata
    46861 aaaagaggcg gcaccttctt tatctgcgcg acgagaaacg gctttcaccg cttcgccaat
    46921 agcattaaaa cgaccggtaa ccactacacg gtcaaaaggt ttaaccgctg ccgcttgctc
    46981 cggtgtcagt tctgttgctg cgttaacgga gaatgccgta gcagaaagca gtgccgacgc
    47041 caggagggtg ttcttaagct tcataaaaat aatccttcgc cttgcgcaaa ccaggtactg
    47101 gtattgttat taacgagaaa cgtggctgat tattgcattt aaacggtgta actgtctgcg
    47161 tcatttttca tatcacattc cttaagccaa ttttaatcct gctcaaatga ccgtctatgc
    47221 ttaaaaaaca gccgtatcag catcaatact actgaagcaa ctgaattgta taagttaatt
    47281 taatgttaag tagtgattcg taccggggcg atgtctcgtt ttacctgacc gtcgaagaca
    47341 attatcagtc tttatccggc gttctaaggt gtttatccca ctatcacggc tgaatcgtta
    47401 atattttgcg agttcacgcc gaaatactga tttttggcgc tagatcacag gcataatttt
    47461 cagtacgtta tagggcgttt gttactaatt tattttaacg gagtaacatt tagctcgtac
    47521 atgagcggct tgtgtggctc ctgacacagg caaaccatca tcaataaaac cgatggaagg
    47581 gaatatcatg cgaattggca taccaagaga acggttaacc aatgaaaccc gtgttgcagc
    47641 aacgccaaaa acagtggaac agctgctgaa actgggtttt accgtcgcgg tagagagcgg
    47701 cgcgggtcaa ctggcaagtt ttgacgataa agcgtttgtg caagcgggcg ctgaaattgt
    47761 agaagggaat agcgtctggc agtcagagat cattctgaag gtcaatgcgc cgttagatga
    47821 tgaaattgcg ttactgaatc ctgggacaac gctggtgagt tttatctggc ctgcgcagaa
    47881 tccggaatta atgcaaaaac ttgcggaacg taacgtgacc gtgatggcga tggactctgt
    47941 gccgcgtatc tcacgcgcac aatcgctgga cgcactaagc tcgatggcga acatcgccgg
    48001 ttatcgcgcc attgttgaag cggcacatga atttgggcgc ttctttaccg ggcaaattac
    48061 tgcggccggg aaagtgccac cggcaaaagt gatggtgatt ggtgcgggtg ttgcaggtct
    48121 ggccgccatt ggcgcagcaa acagtctcgg cgcgattgtg cgtgcattcg acacccgccc
    48181 ggaagtgaaa gaacaagttc aaagtatggg cgcggaattc ctcgagctgg attttaaaga
    48241 ggaagcgggc agcggcgatg gctatgccaa agtgatgtcg gacgcgttca tcaaagcgga
    48301 aatggaactc tttgccgccc aggcaaaaga ggtcgatatc attgtcacca ccgcgcttat
    48361 tccaggcaaa ccagcgccga agctaattac ccgtgaaatg gttgactcca tgaaggcggg
    48421 cagtgtgatt gtcgacctgg cagcccaaaa cggcggcaac tgtgaataca ccgtgccggg
    48481 tgaaatcttc actacggaaa atggtgtcaa agtgattggt tataccgatc ttccgagccg
    48541 tctgccgacg caatcctcac agctttacgg cactaacctc gttaatctgc tgaaactgtt
    48601 gtgcaaagag aaagacggca acatcactgt tgattttgat gatgtggtga ttcgcggcgt
    48661 gaccgtgatc cgtgcgggcg aaattacctg gccggcaccg ccgattcagg tatcagctca
    48721 gccgcaggcg gcacaaaaag cggcaccgga agtgaaaact gaggaaaaat gtgcctgctc
    48781 accgtggcgt aaatacgcgt tgatggcgct ggcaatcatc cttttcggct ggttggcaag
    48841 cgttgcgccg aaagagtttc ttgggcactt caccgttttt gcgctggcct gtgttgtcgg
    48901 ttattacgtg gtgtggaatg tatcgcacgc gctgcataca ccgttgatgt cggtcaccaa
    48961 cgcgatttca gggattattg ttgtcggagc actgttgcag attggccagg gcggctgggt
    49021 tagcttcctt agttttatcg cggtgcttat agccagcatt aatattttcg gtggcttcac
    49081 cgtgactcag cgcatgctga aaatgttccg caaaaattaa ggggtaacat atgtctggag
    49141 gattagttac agctgcatac attgttgccg cgatcctgtt tatcttcagt ctggccggtc
    49201 tttcgaaaca tgaaacgtct cgccagggta acaacttcgg tatcgccggg atggcgattg
    49261 cgttaatcgc aaccattttt ggaccggata cgggtaatgt tggctggatc ttgctggcga
    49321 tggtcattgg tggggcaatt ggtatccggc tggcgaagaa agttgaaatg accgaaatgc
    49381 cagaactggt ggcgatcctg catagcttcg tgggtctggc ggcagtgctg gttggcttta
    49441 acagctatct gcatcatgac gcgggaatgg caccgattct ggtcaatatt cacctgacgg
    49501 aagtgttcct cggtatcttc atcggggcgg taacgttcac ggggtcggtg gtggcgttcg
    49561 gcaaactgtg tggcaagatt tcgtctaaac cgttgatgct gccaaaccgt cacaaaatga
    49621 acctggcggc tctggtcgtt tccttcctgc tgctgattgt atttgttcgc acggacagcg
    49681 tcggcctgca agtgctggca ttgctgataa tgaccgccat tgcgctggta ttcggctggc
    49741 atttagtcgc ctccatcggt ggtgcagata tgccagtggt ggtgtcgatg ctgaactcgt
    49801 actccggctg ggcggctgcg gctgcgggct ttatgctcag caacgacctg ctgattgtga
    49861 ccggtgcgct ggtcggttct tcgggcgcta tcctttctta cattatgtgt aaggcgatga
    49921 accgttcctt tatcagcgtt attgcgggtg gtttcggcac tgacggctct tctactggcg
    49981 atgatcagga agtgggcgag caccgcgaaa tcaccgcaga agagacagcg gaactgctga
    50041 aaaactccca ttcagtgatc attactccgg ggtacggcat ggcagtcgcg caggcgcaat
    50101 atcctgtcgc tgaaattacc gagaaactgc gcgctcgtgg tatcaacgtg cgtttcggta
    50161 tccacccggt tgcggggcgt ttgcctggac atatgaacgt attgctggct gaagcaaaag
    50221 taccgtatga catcgtgctg gaaatggacg agatcaacga tgactttgct gataccgata
    50281 ccgtactggt gattggtgct aacgatacgg ttaacccggc ggcgcaggat gatccgaaga
    50341 gtcctattgc tggtatgcct gtgctggaag tgtggaaagc gcagaacgtg attgtcttta
    50401 aacgttcgat gaacactggc tatgctggtg tgcaaaaccc gctgttcttc aaggaaaaca
    50461 cccacatgct gtttggtgac gccaaagcca gcgtggatgc aatcctgaaa gctctgtaac
    50521 cctgacggcc tctgcggagg ccgtcactct ttattgagat cgcttaacag aacggcgatg
    50581 ctttgacctc ccgctgtttg ttcaagcgca attttgacaa taattgtcaa cggcacggaa
    50641 agcagcatac ccaccggtcc taacaaccat ccccaaaaaa tcaacgacag aaataccacc
    50701 aatgtggaaa gccccagccc acgccccatg atgcgcggct ccagaatatt gccgaagacc
    50761 agattaatca gcagatatcc cgccagcacc agcaacgcgt cgtagaagcc attaaatacc
    50821 agtacctgag cgataggggg aattgccgcg agaactgaac caatattagg gatgtaatta
    50881 agcgcaaagg ccagcaatcc ccagacaaaa gcgaagcgaa catcgagtgc ggcgagcatc
    50941 gcccaggcga ccaggccggt gatgatgctg atggctgttt tcagcaccag ataatgagac
    51001 acactgtcaa tcgcacgctg aatcgccgcc atcccttcaa ccggacgcgc catcatttgc
    51061 tgaaattttc cgggcaattg tggcacttcg agcagcataa acagcaccgt cagcagcaat
    51121 aaaaatattg atgacatggc attagataac tgcgtcaata agttggtgag caacgtcatc
    51181 gccgcgttcg gatcaatgta atgcgccagt tggtcaactg agacatcaat ccctacgcgt
    51241 tgcaacaacg gttcaatcgc ttgcagcggc gtcataatag agttgcgata ttgcggtaac
    51301 gtccgcgtca actcgttgag cgcggaaccc agataagcta acagcaacac catcgccatc
    51361 acgatgatgg tcatcaaaat cgacaccgcc agtacacgcg gcactcgcca gcggaccatg
    51421 tgttgcacca gcgggttaag aataacagca ataaataatg cgagaataaa cggcacgatg
    51481 atctcggcgg caaaacggat accgcagaga ataatgacca gcattcccaa cataatgacg
    51541 atttttaggc cattgagcgt gatgatcggc tttgccatgc ttgtccccgt tttttcttcc
    51601 tttgctaata ataatgtctt tttcgctccg tcatcataac gaaaatcaaa ccgctcaggt
    51661 aaagaagtga aaatattttg agttaattct taagctatga tacaaatcag gcgtgttcaa
    51721 ctaccgagga caattatcat ccgcgatgac gagaagcaac actgcggata attgtaatat
    51781 tatggacaat ttgttcagga cgttattttc tacatttacc catcttcgta cctcatccac
    51841 tatcttgctc gtaggtgagc agcactggcg caacgcgctt tagtcctttc ttctgcttaa
    51901 gcttacgcta tgcgcggggc ttgaccactt tactttggtt tcgctgaatt aagcgaaaat
    51961 taaaataatt ctcttgcagg agaaggacaa tgtatattta ttggatttta ttaggtctgg
    52021 ctattgctac agaaattacc ggtacgctgt ctatgaaatg ggcgagcgtc agtgagggaa
    52081 atggcggctt tattttaatg ctggtgatga tttctctgtc gtatatattt ctctctttcg
    52141 ccgttaaaaa gatcgcctta ggcgtggctt atgcgctgtg ggaaggtatc ggtattttat
    52201 ttattacctt gtttagtgtt ttgttattcg acgaaagttt atcgctgatg aaaattgccg
    52261 ggttaaccac cctggtggcc gggattgtat tgatcaaatc aggtacccgt aaagcgcgta
    52321 aacctgaact ggaggtgaac catggcgcag tttgaatggg ttcacgccgc ttggctggca
    52381 ttggcaatcg tgctggaaat cgttgctaac gtctttttga aattttctga cggctttcgt
    52441 cgcaaaatat ttggcttgct ctccctggcg gcggtgctgg ctgcctttag tgcgctttct
    52501 caggccgtta aagggatcga cttgtctgtc gcttatgcat tgtggggcgg gtttggtatt
    52561 gccgccacgt tagccgcagg ttggatcttg tttggtcaac ggttaaatcg taaaggctgg
    52621 attggcctgg tcttgctgtt ggctggaatg atcatggtga aacttgcctg atgaacacgc
    52681 tgcccgcgct gtcgggcagc gtttgaacat tatttttgcg acagttgatc cagcctgtcg
    52741 cgaaaaccgg taacagaaat ggcccggtta tcggcgcgcc agcgatcttt cgcggcagga
    52801 gccgaacttt gcaccccaat taattgccag ccgtcatcgg tatgcaacat cagaggcgaa
    52861 ccgctgtcac ccggcaaggt atcgcactga tgcgacatca ccgacgtttg cgcccagcca
    52921 gtcacttcac agttttgatg actgtacaac gtatcgagat gatcttcagg gtatcccgcc
    52981 tgagtcactt tacgacctgc cgcttttaat gcggcggtaa gcgcggcttt atctccctca
    53041 aataacggca atggtgtaat gccagaaggc ggattacgta gcacaatcaa accgaagtcc
    53101 cacggcgcag ctgcgggagg aacaatccaa ccatccccat ctgcttttaa ccgctttccc
    53161 agtgtcggat caacgcggcc ttctatgtcg tggatctcat agcgccaaag acctttattt
    53221 gacacgaaac gcagcgctac cgctttatcg gctttaccct ttggcggcgt caataaacag
    53281 tgtcctgccg ttaatgccag attgggtgca atcagcgtcg ccgtacataa attgccgctg
    53341 gccgtttcca gttggccaac cgcatcccac ggtgattggg tcgtgtcatt cactggcaca
    53401 cgatcatcat gaccaaaaaa cagggtgctg acctcatcgt ttgccgattt ggcaacgtct
    53461 ggtttatctg caaacacaaa agcagacgtc aaactaattg cacccaacac tacagcaatg
    53521 gttgtacgca tatcacactc tggtgggtaa ttatgattat taaaagcaaa ccctcataaa
    53581 tactatagac gggacgggat gaaagtggga gtaaaatcag ataactacga tcaggaaaga
    53641 taaaaacggg cggcaatcag tgcgcataaa ataaggatga taagaatgaa ctcaaagcga
    53701 taacggccca ccatactacc ctccgctaaa ggcggtgcta aacgttatcc gcttagcacc
    53761 gtaattttca ggcgcgaggg ggcgcgccag cattactatt gataacttac gctgcgggtt
    53821 gtgcagcagg ttttgccggt tgctgatggc tgtgtttctt ggcgtgtttc ttcgctgcct
    53881 gcgctttttg ttcaggagct ttctgttcag ctttggcatt tttatgatgc tttttagccg
    53941 cctgcgcttt ctgggcaggt gctgctttat gctgtttttt atgatgtgtg gttttcgcag
    54001 gcgctgcttt ggtggtcgtc gcagtcggag caggtgtggt cgcagtctct gcagcaaagg
    54061 cggcagaaga cagacccata gcagcggcaa caaccagagc taatactttt ttcattgtca
    54121 taccctcaat ttgttttttc atttaacccc actgcggggc cgttgaaata actatatccc
    54181 tgggaatggc agacttccgt gagtggttgg tttcagcgtg taacgatatg tacaaacgct
    54241 gaataaatta cagcgttgat aataacgtat tgtgacttaa gggaaattta gctaccaata
    54301 atagtagtct tgatcgggtt aactatttta ctacttacaa agcggtagaa taactgcgca
    54361 tcagtaataa atgacacaca gcaaaatgaa tccgtttatt tgggtactta taatcctgat
    54421 gacactagat gcgctgcggg aattggctgg cgcttcgtct attttaggat ggctattaac
    54481 gttggtttga gcaggcaata agtccggatg ggtatttacc gcagtccgga cttatttttc
    54541 aggcgtgcag acgacgatgc aaacgcgtcc cgaccagcag agcaatgacc agcatcagag
    54601 caataaatgc gccgacgccg ttccagccat agttatgcca gaaaacacca cccagcgtcc
    54661 cggcaatact cgaccccaga tagtaactga acagatacag cgaggaggcc tggcctttag
    54721 cgcgttttgc gcgggggccg atccagctgc tggctactga gtgggctgcg aagaatccgg
    54781 ctgagaagag taacattccg gcaaagatca gccacagcga gctgaataag gtcatcaata
    54841 aaccaaacag cataaccccc gtcgaaaaca acatcaccgg accgcgccca tagcgggtgg
    54901 tcatggttcc ggctttgggt gagctccatg taccggtcaa ataagccagc gataataagc
    54961 caaccacggc ctgactgaca tgccaggggg agagcatcaa ccgatagccg atgtaattaa
    55021 acagcgtgac gaacgacccc atcagcaaaa agccttctgc gaacaataac ggtaatcccc
    55081 ggtcacgcca gtgcagacga aagttgataa ataacgtctt tggtcgcagg gaagttgggc
    55141 gaaaatggcg tgattccggg aggattttcc agaacatcaa cgccgaggcc agcgcgaaac
    55201 aaccgattgc cgccagagca attcgccagt tgaaaaagtc cgtgaagaca ccactaatta
    55261 agcgtccgct catgccgcca attgagttgc cgctgatata caaccccatc gaaaaggcca
    55321 cgaaactggg gtggatttcc tcgctaagat aagtcatgcc aacagctgcc acgccactta
    55381 acgaaagccc aatcaaggcg cgcataatca aaatgccgtg ccagctggtc atcattgttg
    55441 aaagtaatgt gcaaatggag gccaacagta gcgccgttac catcactggt ttgcgaccaa
    55501 tggcatcaga taacgggcca gtaaacagca aaccgatagc caacatcgcc gtagaaatgg
    55561 acagtgaaat actactgttc gcgggcgtta agccaaactc ctgcgaaagc accggaagga
    55621 taggttgcac acaatagaga agtgcgaagg ttgccagtcc ggcagagaac agcgccaggg
    55681 tgacgcgcat aaattgcggc gtaccgcgtt taataaattg atttggctga gaaatgcttt
    55741 gcttgtcagt gtcgcttgcc ggagcgccat caacagttgt agtacggctc acttgaaatc
    55801 cttgctaaat atgcctgtgg atcaggctta tacatagggt aggaaaatcg aattgttctg
    55861 tctaatatat taataatctc aaataagatg ttttaaatat gaatattgaa cttcgtcatc
    55921 tgcgttactt tgttgctgtt gctgaagagc tgcatttcgg gcgcgccgct gcccgcctga
    55981 atatttcgca accgccgcta agtcagcaga ttcaggcgct ggagcaacaa atcggtgccc
    56041 gactgctggc acgaactaat cgcagtgtat tgctgacggc agcaggaaaa cagtttcttg
    56101 cagatagtcg gcaaatcctg tctatggtgg atgacgctgc cgctcgcgct gaaagactgc
    56161 atcagggtga agcgggggag ttgcgcattg gttttacttc gtccgctcct tttattcggg
    56221 cggtgtccga tacgttatcg ctgtttcgcc gtgattatcc cgatgtccat ttacaaaccc
    56281 gcgaaatgaa cactcgcgag caaatcgctc cgctcattga aggaacgctg gatatgggat
    56341 tgctgcgtaa cacagcgtta ccggagacgc ttgaacacgc agtcatcgtc catgaaccgc
    56401 ttatggcgat gatcccgcac gatcatcccc tggcaaataa cccgagtgta acgctggctg
    56461 aactggcgaa agaacccttt gtcttttttg atccgcacgt cgggacaggg ctgtatgacg
    56521 atattctcgg gctgatgcga cgttaccatt tgacgcccgt catcactcag gaggtgggtg
    56581 aggcaatgac catcatcggt ctggtttccg ccggtctggg tgtttcaatt ttgcctgcgt
    56641 catttaaacg tgttcagctc aacgaaatgc gctgggtgcc gattgctgaa gaggatgcgg
    56701 tttctgaaat gtggttggtc tggccgaaac atcatgaaca aagtccggct gcgcgtaact
    56761 ttcgtattca tctgctgaat gctctcaggt gagggaaatt tcagcgaaaa agcccgaaaa
    56821 atgtgctgtt aatcacatgc ctaagtaaaa atttgacgac acgtattgaa gtgcttcacc
    56881 atagcctaca gattatttcg gagcgcgaaa atatagggag tatgcggtgg ttgctgaaaa
    56941 ccagcctggg cacattgatc aaataaagca gaccaacgcg ggcgcggttt atcgcctgat
    57001 tgatcagctt ggtccagtct cgcgtatcga tctttcccgt ctggcgcaac tggctcctgc
    57061 cagtatcacc aaaattgtcc gtgagatgct cgaagcacac ctggtacaag agctggaaat
    57121 caaagaagcg gggaaccgtg gccgtccggc ggtggggctg gtggttgaaa ctgaagcctg
    57181 gcactatctt tctctgcgca ttagtcgcgg ggagattttc cttgctctgc gcgatctgag
    57241 cagcaaactg gtggtggagg aggcgcagga actggcgtta aaagatgact caccattgct
    57301 ggatcgtatc atttcccata tcgatcagtt ttttatccgc caccagaaaa aacttgagcg
    57361 tctaacttcg attgccataa ccttgccggg aattattgat acggaaaatg gcattgtaca
    57421 tcgcatgccg ttctacgagg atgtaaaaga aatgccgctc ggcgaggcgc tggagcagca
    57481 taccggcgta ccggtttata tccagcatga tatcagcgca tggacgatgg cagaggcctt
    57541 gtttggtgcc tcacgcgggg cgcgcgatgt gattcaggtg gttatcgatc acaacgtggg
    57601 ggcgggcgtc attaccgatg gtcatctgct acacgcaggc agcagtagcc tcgtggaaat
    57661 aggccacaca caggtcgacc cgtatgggaa acgctgttat tgcgggaatc acggttgcct
    57721 cgaaactatc gccagtgtgg acagtattct tgagctggca cagctgcgtc tcaatcaatc
    57781 catgagctcg atgttacatg gacaaccgtt aaccgtggac tcattgtgtc aggcggcatt
    57841 gcgcggcgat ctactggcaa aagacatcat taccggggta ggcgcgcatg tcggtcgcat
    57901 tcttgccatc atggtgaatt tatttaaccc gcaaaaaata ctgattggct caccgttaag
    57961 taaagcggca gatatcctct tcccagtcat ctcagatagc atccgtcagc aggcccttcc
    58021 tgcgtatagt caccacatta gcgttgagag tactcagttt tctaaccagg gcacgatggc
    58081 aggcgctgcg ctggtaaaag acgcgatgta taacggttct ttgttgattc gtctgttgca
    58141 gggttaacat tttttaactg ttctaccaaa atttgcgcta tctcaatttg ggccaggaaa
    58201 gcataactta gactttcaag gttaattatt ttcctggttt atatttgtga agcataacgg
    58261 tggagttagt gatgctgaag cgtttcttta ttaccggtac agacacttct gtagggaaaa
    58321 cggtggtttc ccgcgcattg ctacaagcgt tagcctccca gggaaaaacg gttgcgggat
    58381 ataaacccgt agcgaagggg agcaaagaga cacccgaagg gctgcgtaat aaagatgccc
    58441 tggtgttgca gagtgtttca accatcgaac tgccttatga agcagttaat cctatcgcgt
    58501 taagcgaaga agaaagtagc gtggcgcaca gttgcccaat caattacacc ctcatttcaa
    58561 acggcctggc aaacctgacc gataaagtcg atcatgtggt ggtagaaggg actggcggct
    58621 ggcgcagtct gatgaatgat ttgcgtccgc tctctgaatg ggtggtgcag gaacaactgc
    58681 cggtgttgat ggttgtcggt attcaggaag gttgcattaa ccatgcactg ctaacagctc
    58741 aggcgatcgc caacgacggg ctaccgctca ttggctgggt ggctaaccga atcaatccag
    58801 gactggcgca ttatgcggaa atcattgatg tgctcggtaa aaaacttccg gcaccgctca
    58861 ttggtgaact gccttatctg ccgcgcgccg aacagcgtga actggggcaa tacatccgct
    58921 tagctatgct tcgcagtgtg ctggcggtag atagagtcac ggtgtaacgt ccgcgaaatt
    58981 accgacgcaa ttacgcaggc aatcaataaa ccggggagta gctgatactc cccggtcatt
    59041 tcacatatca tcaacgtcga cataatcggc gcgtgcgtgg ttgccgccaa cagtgtcgcc
    59101 atcccggtca atccgagtaa aagcgtaatt tcttcgccat cagggaacca taatcccagg
    59161 ctacgaccat acaacatgcc aatggcaaga ccgataaata gcgtcggtgt aaagacacca
    59221 ccgggcgcgc cagaaccact actcgccagc acggcaaaca gtttacagag gaagatcccg
    59281 gcaatgatca ttaacagtgg cggggcggtt aaaaaggatt gtacggtgct atagccgttg
    59341 ccccacactg caggtgtgaa cagggaaagc agacccacga tcaacccgcc cagtgccagt
    59401 tgccagggcg gcgcaagttt gagacttaca aatccacgat gacaggcgtt cattaacgtt
    59461 aacaacagtg gtccgcacag acctgccagc acacctgtac tgataatcag cgcatagtca
    59521 cgagcctgaa tcgtcactga gagttgtacg ctgtagagta acgcgtcgct atgattaatc
    59581 agattgctaa ccagcaacgc cacgacggcg gaaataatca ccgggccgag agaggccaac
    59641 atcatagtgc caaacagcac ttcggctata aataaactgc cagcaagcgg ggcgcgatag
    59701 gccgcagcca ttcccgccgc agctccacag gcgatccata atttccactc ctggcgtggc
    59761 gtaaaacgtt gggcaaaaca ggaggcggca agggcagcta aaagaatcat cgcaccttcg
    59821 cgaccaattg cactgccgct ggttactacc agcagagagg caagcgattt aaccaggctt
    59881 gctgcgtaat caaactgccc atcggtttgc aacgcttcca tgtaatcggt cggcgcatga
    59941 gggcgttgct gggtaaattt ctgccagccc atcagcaaca aacccgccgc cagtccgccg
    60001 agcgccggag ttagcagccg tcgccacggg gaaagatttg tcgctgcatt gaccagactg
    60061 cccgagtcat tattgaggaa caaccactcc agtagcagca tcgtatgacg aaacccggca
    60121 acggcaaagg ccgcgagaat accgacgact gttgcgataa gcagacggcg gaacatagtg
    60181 cgcagatccg gatagatgtg tagatgatgc atgagcagaa atgggcaatg aaacacgggc
    60241 gactattttg cggcaaaaga ggccagccgc aaaagcagtg cgtgctttaa gagataaaaa
    60301 aacgtgacac tgtaacctat tattgccgaa ttgcgcgtaa tcgtcaccat ccggcaataa
    60361 taaggtgatc ctatcgaaag agtggtttaa ccgcgacagg aattaacagt tgtgattgcc
    60421 attgcgccag cgttaagcga gccagttcac ccagtgcacg gtagaaaggg tgttctgctt
    60481 tttcgataaa aacgtcaaga aaacgtgttg accacggaaa aaggtgccat gccagcaatt
    60541 cttcgcattc tgtctggcga ccattctccg ccaaccacgc agccatcaag agcagtgaac
    60601 caaaatgatc ttccggttcg ttttgcttca tttcaaactg aatgcctttc tcgcgcatcc
    60661 actgacgaag tgccaatgtt gaatcgccaa acagcacaga ttcgcgatcc agccagaccg
    60721 atccccacgg cggagacggc agtgcccacg ggccgacaaa caaacgctgc caggcctggg
    60781 cgcgagtctc ttcactctgt gcctgaaaag ccgtcaccag cggcgctaat gacgcttctg
    60841 gtagaggcca ctgagtttcc cagccgtcac gggtgagtac cgcaaccaga ggtgcggctt
    60901 ccgcactctc tggagcgtaa taaaacagcg cgcccaacac gcgcgccgtg acagaaaaat
    60961 tatcttgctg tgaaaaatgg gtcattccta acatcctgaa atgtgcgggt tgccccgcac
    61021 caatttgtta acctgcaata gccattccta ccgtcatgtg caggccataa aagagcacac
    61081 gcccgatcat ctcaccgtca agtatgagga ttagccccag aacaagtccg gcaacgtgag
    61141 gttcacggcg acgaatgagc ggacaaagcc agcatcctag acctgtgcat aacaatacca
    61201 cacgccagac ctgtaaggag gcgtaatctg gtaccagtgc gctggcttgt tgtacggatg
    61261 agtgaataga cgccagagac aaaccttgca gcacaatcac cccggcacaa gcgatcagcg
    61321 ccagaacgct aatgatggca aatggtgtgg tattaaatgt aacgcgggct gcccgcagaa
    61381 ttgcagcagc aagtattgga ccgctcaata atactgtcag gaagaaagcc agcgtagtgt
    61441 aaccgttatg ccaggttggc actgtgtcga tttgatacac gcaggtcatc atccagacga
    61501 aaatgacgcc gagcgccatg ctgacaagta accagagttt ccccaacgct tgcggcattt
    61561 taccgatgac tgccaccagc caccataatc cgccaacggc aaaaaatatt gaaccggcag
    61621 caatttcgtt actcagacca gaagcaccga ttcgattcag cgagttaaac gctcgcagtg
    61681 gcgatcccag gtgcatgaca gaggcaataa acccgatgcc catcaacagc cagaggaaaa
    61741 acatgccacg gacaatacgc tgacggtctg cgtcattatt ggcggcgaac cagccaattc
    61801 cgctaacaat cagcgcgcct actacgcatt gacccaatac ggtaaagata accagtggcc
    61861 attcatgcca tccatttccc attttacact tcctccggat tagccagata accagtggta
    61921 tcgccggtcg ggcggctgtt ggcgttaggt ttgataacga tattgggttt tgtgaaatgc
    61981 gcgcgcggca gtggcgcgac ggcagccaga gtgccgtgct tctggcgcag ctcatcaatt
    62041 ggaccgaact caagtgcacg cagcgggcag gattcgacac atatgggttg tttcccctcg
    62101 gcgacgcgcg aataacaacc atcgcacttc gtcatgtgcc ctttttcagc attgtactgt
    62161 ggcgcgccgt acggacaagc catgtggcag tagcggcagc cgatacaaac atcttcatca
    62221 accaccacaa agccatcttc acgcttatgc attgcgccgc tggggcagac ttttgtacat
    62281 gcggggtcat cgcaatggtt acaggagatg gagagataat aagcgaacac gttctggtgc
    62341 cagacgccat tatcttcctg ccagtcgccg ccagcatatt cataaatacg gcggaaactg
    62401 acttccgggc caagatcttt aaaatctttg cacgccagtt cgcaggtttt acagccagta
    62461 cagcggctgg aatcgataaa aaatccatat tgtgtggtca tgggctactc cttaaacctt
    62521 ttcgatctgg acaagattgc tgtgcgacgg gtttcccttt gccagcggtg aagggcggtg
    62581 agaggtcaga atattgatac tgccgccgtg atcgacccgg tcaccaaaca tatccgcttt
    62641 aagccacgca ccttgcccga tggcggtaac gccaggcaga atacgcggag tcacttttgc
    62701 ggcaatcagc atttctccat tattgttaaa tacccgcacg gtatcgccat gacggatacc
    62761 gcgtgcctga gcatcaatgg ggttgatcca cacctcttgt gggcaggcct gctgtaacac
    62821 atcaatattg ccgtagctgg agtgggtacg cgctttgtag tggaagcccg ttagctgcag
    62881 tggataggtt ttccgcaggg gatcgtccca gccatcaaaa cctggggtat acgcaggaag
    62941 gggatgaata atttcatctt ttttcaattc ccaggtatct gcaatcttcg ccagtcgttc
    63001 agaataaatt tcgattttcc ccgaaggtgt tttcaacggg tttgcctgtg gatcttcacg
    63061 gaatgcgcgg aaagcgacgt agtgttcttc cgggcatttt ttcttaaaga tcccggtcgt
    63121 tttcatctcc tcgtagtcgg gcatctcagg gttacgttcc ttcgttttcg catggagata
    63181 tttgatccat tcgtgctgac tgcggccttc agtaaaggtt tgataaacgt ctggtcctaa
    63241 gcgtttggcg acttcgctca gcatccagta gatgggtttg cgttcaaatt ttgctgaggt
    63301 tgcgggttgg gcgaggatca catagcccat attcccggca gattcgtgag agataaggtc
    63361 ttcttgctct gttggcatca ggtcgggcaa caggatatcg caatacttag ccgaggcagt
    63421 catgaagtgg tcaatgccaa caatcatctc gcacttgctg tcatcctgaa gcacctcatg
    63481 ggtgtgattg atgtcgccat gttgattgat caatgtgtta ctggcgtagc accataaaaa
    63541 cttgatgggg acatccagtt tttcttttcc acgaacacca tcacgggtcg cggtcatttc
    63601 cgtaccgtgg tcgatggcat ctgtccatgt aaagacggaa atctgcgttt taacaggatt
    63661 ctcaagcatc gggaaccatt ctacccccag atcccagcta ccttcgcgta cacctgagtt
    63721 gccgccgttt atgccgacgt taccggtgag aacggaaagc atggcaatag cgcgggacgt
    63781 ttgttcgccg ttggagtgtc gttgtggccc ccaaccctga caaatataag caggttttgc
    63841 tgaaccgatc tctcgtgcca actggataat tttttctgcc ggtatgctgg tgatttttgc
    63901 tgcccattcc ggcgttttag ctatgccgtc aggcccttcg cccagaatat aggctttata
    63961 atgcgcgtta cgtggtgcgt tggcgggcag cgttttttca tcgtaaccaa cacaatattt
    64021 gtcgagaaat ggctgatcga ccatgttttc agtaatcagt acccaggcaa tcgcacaggc
    64081 cagtgcgcca tcggtgccag ggcgaatggg cagccattca tcttcacgcc cggcagcagt
    64141 gtcgttataa cgtggatcga tgacgatcat gcgtgcgttt gaacgttcgc gggcttgctc
    64201 gacgtagtaa gtgacaccac cgccgctcat ccgcgtttct gccgggttat ttccgaacat
    64261 aacgaccagt ttcgtattgg cgatatcatc cgggctgttg ccatcattgg caccgaacat
    64321 ataattcatc gcggcactga tctgtgcggt actgtagcta ccatagcgac tgagaaaacc
    64381 accgcaagag ttcatcagac ggtacgggac gtttgagttg gtgatgtttc cgccatctac
    64441 gcctgttccg tacaggacat gtacagcctc attgccgtaa tctttaagga tccgccgaag
    64501 attatcactg atggtatcca gggcttcatc ccaacttatc cgttcaaatt taccttcacc
    64561 gcgcttgccg acgcgcttca tgggatattt caacctgtca ggatgattca tccgtcggcg
    64621 gatagagcgc ccgcgtaaac atgcccgaac ctgatgatta ccgtagacgt cgtcacctgt
    64681 cgtatcagac tccacccagt acacggtgtc atctttcaca tgcaaacgta acagacagcg
    64741 gctcccgcag ttaacggtgc aggaactcca gaccgctttc tcttctaccg gtgcctctgc
    64801 cgctcggacc atttgggaaa atggcagagt gaaagcactg cttgccagcg caagactgcc
    64861 aagtgcggag gttttcatca gacttctacg gctgatttca gccttcatga gcgcctctgt
    64921 ggtatggatt ttcatcatta ctcacttatt gcttttcaaa caaaatgtca tgccagaatt
    64981 tatggttgta gtgggttata ttttttcgat ctcgaccaga ttagtgtgct gcgggtttcc
    65041 cttcgccagt ggtgaagggc gcagagtggt tagcgtattc acacagccgc catggtcgat
    65101 tttatcgcca gacatattgg cctcgtgcca ggctccctgg cccatagcgc taaccccagg
    65161 gagaatgcgt ggtgtcactt tggctggtag ccgaacttcg ccacgatggt taaacacccg
    65221 caccatatcg ccgttggcaa tcccacgttt ctgtgcatct atagggttga tccacacctc
    65281 ctgacggcag gctgccttca ggacatcaat attgccgtag gtcgagtggg tacgggattt
    65341 gtaatggaaa ccaaacagtt gcagtgggaa ggttctacgt tcaggggagt cccagccttc
    65401 aaaggttgag gcataaactg gcagtgggct tatcacttca tctttttcca gttcccaggt
    65461 acgggcaatt tccgccagct tgctggaata aatttcaatc ttaccggaag gcgttttaag
    65521 tggatttgcc tcggggtcgt cacgaaatgc tttgtaggcg acaaaatgac cattgggatc
    65581 tttacgctta tagataccca tttttttcag ttcgtcgtaa gacggtaacg ccggatcttt
    65641 ggcaagcatt ttggcgtaca gatgttgtaa ccattgttcc tgcgtgcgac cttctgtgaa
    65701 cttttgatag acgtcaggtc caagacgttt cgcgacctca ctcaggatcc agtaaatcgg
    65761 tttgcgttcg aatttttcgc tggtgactgg ctggaggaaa atgagatatc ccatgttacc
    65821 ggcgtagtcg ttaggaataa tatcttcctg ctcaacggtc atcaggtctg gcagcagaat
    65881 gtcggcatat tttgccgatg aggtcataaa gttttcgatg accacaatca tttcgcattt
    65941 cgattcgtcc tgcagaattt catgcgtttt gttgatgtca gaatgctgat taacgagggt
    66001 atttcccgcg tagttccaga tgaacttaat gggcacatcc agtttatctt tgccgcggac
    66061 gccgtcgcgg attgccgtca tttgcgggcc atgatcgata gcatctgtcc agctgaagca
    66121 ggagattgac gttttgaccg gattatcaag caccggcagg cgttctatgg taatggtata
    66181 ggtcgattca cgcgcgccac tatttccgcc gctgatgccg acattgcccg tcagaatagg
    66241 caacatcgca atagcgcgtg cagtcagctc gccgtttgcc tggcgttgcg gtccccagcc
    66301 ctggcagata taagcgggtt ttgctgtgcc aatttcacgc gccagtttga tgatacggtc
    66361 caccgggata ccggtaattt gcgaagccca ctgcggcgtt ttcgctgttt tgtcgtctcc
    66421 ttcaccaaga atataggctt tatagtgacc attttttggt gcatctgcgg gtaaggtttt
    66481 ttcgtcatag ccgacgcagt atttatcgag aaaaggttga tcaacgagat tttcgttaat
    66541 caatacccag gcaatacccg caaccagcgc ggcatcggtg cccgggcgaa taggtagcca
    66601 ttcgtcttca cgaccagcag ccgtatcggt atatcgcgga tcgataacaa tcatttttgc
    66661 gttcgatttc tcgcgcgctt tttcaagaag ataagtgatg ccaccgccgc tcatgcgggt
    66721 ttctgccggg ttgttaccaa acatcacgac cagcttgctg ttttcaatat ccgtggtgct
    66781 gttgccatca ttactgccgt aggtgtaggg catggcacag gaaatttgcg cggtgctgta
    66841 ggagccatac tggttgagtg aaccgccgta gcagttcatc aggcgtttga ccgccgaggc
    66901 tgatggtgaa gagcgggtca tattgccgcc aacaatcccc gaagagtact gaatatatac
    66961 agcctcattg ccatattgtt cgacggtttt tttcaggcta ctggcgatag tatccagggc
    67021 ttcatcccag ctaatccgtt cgaatttgcc ttcgccgcgt ttgcccacgc gtttcattgg
    67081 gtaattcaag cgatcgggat gattaatacg ccgacggatg gagcgaccgc gcaaacaggc
    67141 acgtacttga tgattgccat actcgtcgtt gccggtgttg tcagtttcca cccaggtcac
    67201 ttcattatct ttaacatgca gacgaagtgc acagcggcta ccacagttga cggaacaggc
    67261 accccagacc actttctcgc tggcctgttg taccgctgcc gctgcactgc gcagggtgaa
    67321 cggtaaagaa aaaccgcctg cagccagcgc cagagaacct atcgcggtag atttaacgag
    67381 tgttctgcgg ctgatgccca ccatttgttc atttttggac ataactcact ccctgttctt
    67441 tatcgttata taaaagttta tatattgaat atttagcgcg ctaacaatag agggagtcta
    67501 cctattttgg gttaagaatt attaatccat atcaatagaa gggtatgagt aataaggtgg
    67561 gattatgttg tatgttcaaa tcgccggatg tgtcgtatcc ggcgtgcagt cgataatgta
    67621 ttactgcggt tcggcaggcg cgccatcctg ggtagactgc gcgggagcag agacgttacc
    67681 gctggtggtg cgggtgtaga gaattttatg cgtatcatta gcgcaatgcc cgacgacctg
    67741 ggaatcaggc tgatcaacct ggtcattggg tacaatactt aacgtgaagc tgctttcggg
    67801 tacgccatta ttgataatgc gctgtgatat atcgctctgt atgcgctcac aggatcccgg
    67861 cgcggcgagt accgcgggtg aggcgagggc gagcagaagc gcggcacagc aggttgagag
    67921 tttcatcata agctccttac gcgaagataa cttctttaag catagcattt aacgtgtaaa
    67981 gtactgtatt tgctactatg attgagaatc atctctactc tctggtgact gttgtgaaat
    68041 acaaattact gccatgctta ctcgcgatat tcctcacagg atgtgaccgc acagaggtaa
    68101 cactttcatt tacccctgag atggccagtt tctctaatga attcgatttt gatccgctgc
    68161 gtggtccggt aaaagatttc actcagacat taatggatga gcaaggtgaa gtgacgaaac
    68221 gtgtttcggg gactttgtcg gaagaaggct gttttgattc actcgaatta ctggatctgg
    68281 aaaataatac cgtggttgcc ctggtactgg acgccaatta ttaccgtgat gcccagacac
    68341 tggagaaaag agttcgtttg cagggaaaat gccagctagc agaattacct tctgcagggg
    68401 tgagttggga aaccgatgat aatggctttg tgattaaagc cagcagcaaa cagatgcaga
    68461 tggaatatcg ctatgatgat cagggttatc cgctgggtaa aaccacgaaa agtaacgaca
    68521 aaacattatc tgttagcgct acgccatcaa cggatccgat caaaaaatta gattacacag
    68581 cggttacttt actgaataat caacgagttg gtaatgtaaa acagagctgt gaatatgaca
    68641 gtcacgccaa tccggtggac tgtcagctaa tcattgttga tgaaggagta aaacccgccg
    68701 tcgaacgggt ttacaccatc aaaaatacta tcgattatta ttaatgctat tgtgcggtcg
    68761 gcttcaggag agtctgaccc ggtgttttgt gctctgccag atactgatgc tggaatatgc
    68821 acatgcgaat ggcattccga tattgaccat taataaagaa ctcatgcatc aattcacctt
    68881 caaccgtaaa gccaagcttg cggtaaatgt gaatcgcttt ttcattctct ttatcgacga
    68941 tcagatacag cttatagaga ttaagaacgg tgaagccata gtccattgct aattttgcgg
    69001 cacgcgttgc cagacctttc ccctgatatt ccggggagat aattatctga aattctgcgc
    69061 ggcgatgaac atggttaatt tccaccagct ccaccagacc ggctttttcg ccgtcacatt
    69121 ccaccacaaa gcgccgttcg ctctgatcgt gaatatgctt atcgtacaga tcagagagtt
    69181 caacaaaggc ttcgtagggt tcctcaaacc agtaacgcat cacactggcg ttattgtcga
    69241 gttgatgtac atagcgtaaa tcttcacgct ccagcgggcg tagcttaaca ctgtgggcgc
    69301 ttggcataac gtgtccttac attccttaaa tcaataacag gttagggggt aataacgcgg
    69361 ccagttcgac ggtccaggca gcgcaaagta tttggctccc agtaggcatt gatgttggcg
    69421 ctttgctcac atttatcgcg gttatcaaaa gcggcgtcgg ctttatccca ctctttttca
    69481 gtgcgtttat tcactttctg acgcagattg cgcgtgtcat tccattgctc tttttccata
    69541 gcggcgtgct ggcggctttg tgcactgtcg ccagactcaa tcaccagttt gttagtttcg
    69601 gcatgaacag ttgtgctcaa tgccattgcg caaggcagca gaaaagcgag caggccaatt
    69661 cgtttgctga gagtaatttt cataactcat tccctgtatg aatgattaaa ggtgattcta
    69721 caccatccac tgcgaacgca aaacgtacca ggaggatgtt tatattgatg atattatgtc
    69781 gtcctataac tatacatgat gtcaataagt gacaaagatg attaaaacaa cgttactatt
    69841 ttttgctact gcgctgtgtg aaattattgg atgctttctg ccctggttgt ggttaaaacg
    69901 aaacgccagt atctggctgt tgcttccggc ggggatttct ctggcactgt ttgtctggtt
    69961 gttaacgttg catcctgcgg cgagtgggcg tgtttacgcg gcttatggtg gcgtttatgt
    70021 ctgcacggcg ttgatttggc tgcgtgttgt ggatggcgtg aaactgagtc tttatgactg
    70081 gacgggggcg ttgattgcgc tttgcggcat gttgatcatt gttgcgggct gggggcgcgc
    70141 gtaaggacat aaatccattt tatcaataag atacaagtaa gtgtcagctg acaaaagata
    70201 ttctatttca tcttttgtca accattcacg gcgcaaatat acgccttttt ttgtgatcac
    70261 tccggctttt tttgatcttt atacttgtat ggtagtagct cagttgcgta gatttcatgc
    70321 atcacgacaa gcgatgcaag gaatcgaaca tgaagatcgt aaaggctgaa gtttttgtta
    70381 cctgtccggg gcgtaatttc gtcacattaa aaatcaccac tgaggacggt attacgggcc
    70441 ttggggatgc caccctcaat ggacgtgagc tttccgtggc ctcttatttg caggatcacc
    70501 tttgtccgca gcttattggt cgcgatgcgc accgtatcga agatatctgg cagtttttct
    70561 ataaaggtgc ttactggcgt cgcggtccgg ttacgatgtc ggccatttca gcggttgata
    70621 tggcgctgtg ggatattaaa gccaaagctg ccaacatgcc gctttaccag ttactcggcg
    70681 gcgcgtctcg tgaaggggtg atggtttatt gccataccac cggtcacagt attgatgaag
    70741 ctctggatga ttatgcccgt catcaggagc tgggattcaa agccatccgc gtgcagtgcg
    70801 gaatccctgg tatgaaaacc acctacggca tgtcgaaagg caaaggtctg gcttatgaac
    70861 ccgcaaccaa aggacagtgg ccggaagagc agctgtggtc gacggagaaa tacctcgatt
    70921 tcatgccgaa attgtttgac gcggtacgta acaagtttgg ttttaatgaa catttgctgc
    70981 atgacatgca ccatcgctta acgcctattg aagcggcgcg ctttggtaaa agcattgaag
    71041 attatcgcat gttctggatg gaagacccga cgcctgcgga aaaccaggaa tgcttccgtc
    71101 tcattcgcca acataccgtc acacccatcg cggtgggtga agtcttcaac agcatctggg
    71161 actgcaagca actgattgaa gagcaactta tcgattatat ccgcactacg ctgacccacg
    71221 cgggtggcat taccggtatg cgccggattg ccgattttgc ttcgctgtat caggtacgta
    71281 ctggctcaca cggtccttcc gatttgtcac cagtctgcat ggctgcggcg ctgcactttg
    71341 atctgtgggt ccccaatttc ggtgtccagg aatacatggg ttattccgaa caaatgctcg
    71401 aagtcttccc gcacaactgg actttcgata acggctatat gcatccggga gacaaaccgg
    71461 gtcttggcat cgaattcgat gaaaagctgg cggcgaaata tccctatgaa cctgcttatc
    71521 tgccagtcgc acgtctggaa gatggcacgc tgtggaactg gtaaggagta agataatgaa
    71581 aagcatttta attgaaaaac cgaatcaact ggcaattatc gaacgcgaaa tacctacccc
    71641 gtcagcgggt gaagtacgag taaaagtgaa acttgccgga atttgtggtt cagatagcca
    71701 tatttatcgt gggcataatc cttttgcgaa atatccgcgc gtcattggac atgaattttt
    71761 tggcgtcatt gatgcagtgg gtgaaggcgt ggaaagcgcc agagtcggtg aacgcgttgc
    71821 tgtcgatccg gtggtcagct gtgggcattg ctatccgtgc tctataggta agccgaacgt
    71881 ttgtacaaca ctggctgtat taggtgtgca cgctgacggt ggtttcagtg aatatgccgt
    71941 ggttccggca aaaaatgcgt ggaaaattcc tgaagcagtg gccgatcaat atgcggtgat
    72001 gatcgaacct tttaccattg cggcgaacgt aaccggtcat ggtcaaccga ctgaaaatga
    72061 taccgttctg gtttacggtg ccggtccaat cggcctgacg atcgttcagg tattaaaagg
    72121 cgtctataac gttaaaaatg tgattgttgc cgatcgcatt gatgaacgac tggaaaaagc
    72181 gaaagagagc ggggctgact gggcgattaa taacagccag acaccgcttg gcgagatttt
    72241 cgctgaaaaa ggcataaagc cgacattaat tatcgatgcg gcttgtcatc cttctatcct
    72301 gaaagaggcc gtaactctgg cttctccagc ggcacgtatt gtattgatgg gcttctccag
    72361 tgaaccgtct gaagtgattc agcaaggaat taccggaaaa gaactctcta ttttctcttc
    72421 acgcttaaat gcaaataaat tcccggtcgt tatcgactgg ttaagtaaag ggttaattaa
    72481 accagaaaaa ttaattaccc atacgtttga tttccagcat gttgctgatg ccattagttt
    72541 atttgaacag gatcaaaaac attgctgcaa agtcttactc actttttctg aataatacca
    72601 ataacggcga gtaagtagta cgcatcttac ctctttttta gagataacca ttatgacaat
    72661 agaaaaacac gaaagaagca ctaaggattt ggtgaaagca gcagtatcgg gatggctggg
    72721 cactgcgctt gaatttatgg atttcaagag tcatacgtgt caactatttg ataaatatta
    72781 aattaatttt tcattgcttc gttatggggc atggttgggg caaactcgct taactgtgta
    72841 tttaacaaag ctacctgtgc attattgttt tcagacatcc attttccgta tacctgaaat
    72901 accatttgcg catctgcatg gcccatctgg tttgctataa atgccgggtt agcaccagct
    72961 gtcagcgacc agcaggcata agtatgtctc gactgatatg attttcgatg gcggagtccg
    73021 gcacgtttta tcgctgcgtc ccacatctgc cttattgagt caacggtaaa atggtcacca
    73081 taatttttta ctctcgctga cacttcaggt tgaaaaacaa aggtgcattt atgtttttct
    73141 gttctgccat actctctgag gtgaacatca atgatatgct ctttgctcag tctcgttaat
    73201 gtcatctgac tccggagagc gtcgattgct ggcttaataa gatgaatgac ccgattggtt
    73261 cccgcctgtg tttttggtac cgtgaaacgg tcttttgcta aatttctcct gatcatcatt
    73321 gttccatttt tcagatctat gtcctcccat ccaagtgcac acagctcacc agggcgaacg
    73381 ccagtataaa cagaaacaca ccataaattt ttggcttgct gatttctgca cgcatcgata
    73441 agacggatga attcctcccg cgaaagagga tccggaatgg ttcttgattc ctttaatggc
    73501 gagatcccct taaacgggtt atctaccagg taaccgttat caacaccaaa ctggaacacg
    73561 gcgttaagat ttgtcatgta attatttaca gttacagccg atctccctgg ttgtgtaaca
    73621 atatagttac ttttggggat ctggtatcca gtcagtagct ctttacgaac ctccagtaat
    73681 ttttctttat taatcgatga ggcaagattt ttttcaccga ttatgctcag gatatttttg
    73741 atgacggcac ggtatgtgtt gagtgatgtt tttgcgactt cagtttcttt cagtgccaga
    73801 aatttttcag ccagttcttt tatggttaaa tcttgtcggg tctcaccaaa tttttccaga
    73861 ttgcgtgagg agggaaactg ttttgcatag tcgaaaacac cagtttttat tgcgtaacaa
    73921 acagaggcgc gtagctcacc tgcaacgcgc ctgttttttg ctgtgtcagg aacccccagg
    73981 ttttccctga ctcttacgcc tttataaaca aaccagatac gtaatttccc tccatggttt
    74041 tccacgcctg tcggatattt catttcaact tctctcatta gttagtgtgg cttttagtca
    74101 agtaagatga cgtcttggtc ttgctgatgc ctggcgctca atccagcgat caatttcttc
    74161 caggttgtaa aagcatggac tgttatccca tggcataccg tcatgagcga catgcttata
    74221 ttcccttcct tccataaacg atttttcccg ggcctttttt aacgtacctt tttttattcc
    74281 tttcagcgca attaactgct cttcggatac ccatttgccg ggagagacaa tcatgattac
    74341 ttcgctcatc gatttcttta tctcttacat tagacgagcg ccggttgcag aataccagtc
    74401 acaaccggcg acagttgaac attaagaatc agcctgactc gggatcagtt tttgccagat
    74461 aactgaaacg tattttgcct ggtaacgggc gtcatcaagt gcattatggc gctcaccttc
    74521 gaattgaata gccgttctgg catcgaagtc tatggctttc cccagctcaa cgattgtgcg
    74581 tacatcgcga tcgttgtagt aacgccacgg gcaggggatc ccctgccgtt cgtatgaacg
    74641 gcgcaaaatc gtgttgtcga agttggctcc atttccccag acctgaacaa aaaattcacc
    74701 ggagttttcg tcgataaatt cccgcaattg taacagtgca tcatctaacg ggatttcatc
    74761 ggtcataatg gcagattgcg cttcgcgtga ttgcttaagc caccatttaa tggtgtcccg
    74821 atcaatgact ccgccagcag tttccagatc gatagtctta ctaaattccg gtcccatatc
    74881 tccggtttgc ggatcgaaaa atattgcacc tattgagatg atcggggcat caggattttt
    74941 tcccatggtt tcaaggtcga tcattagatg gtcacacgtc ctgctggtgg atgtgatttc
    75001 gtgatgaccg ttcaccttaa ttgagtgatc tgccgtctcg ccagtttcat tatcgctatc
    75061 gtgatgctga ttgccgccag tgttctcctt gtgtggatgt tcagcgcctt ccattttctc
    75121 cggatcatct tcctgaactt caacctgata ctcttcatcg aatgtttcct ggtatgttgc
    75181 gtcgcccatt accgcgccac aatcagggca gttgccgccg ccggtctgac cgcaggcggt
    75241 gcagactttt tccacttcct gttgcgccac tgattcaggc tgtttcgttt ctggctcgtt
    75301 ttgtaacgca tttgggctgt tttgttccgc tttttggtag ttccgttccg attcatgctg
    75361 gttctggttc acagaatcgc gggtctggat ccccttaacc catttcggat cattcgggtc
    75421 actaatccct tcaacaaatt caccacgtga tgcagcaagc aacttatcgg cgtcaggctg
    75481 gatgatattg gctgcctgca taatgttgtt tacttcgtca gcggtaactt tcatcggctc
    75541 tggttgttct gaatcttcag cggtatctac attttgcggt aagcccgtgt atgtgccatt
    75601 ttttcgggca aaatattctt cttttgtgat ttcagtggcg ccagcagcca gtgccttatc
    75661 cagaccagaa agtttgtttg cgcgaccgta tttttctccg tccttatctg cgaagaggaa
    75721 atagaacggc ccctcacgct ctacagatgg ttcagcttcc ggcgcggttt cattttttgg
    75781 gatatcagat acctcagttt ccactgcatc agtttgtgtt tctgatgact ggagaacatc
    75841 aacagtgccc aggtctgttt cttcattctc aaacacgccc tttgtcgtca ggtattcgca
    75901 gatatatttg ttcagtgcta cgggatcttt gtgaatgtcg atcggacgtt cacggacaag
    75961 gccaaaaata gtttggcggt cgtagcgaag ggcatcaggc tgtttgcgca ttgatgccga
    76021 gatacgcttc cagtcttcgc ggtcgttatc gataacttca ttttttgccc agcgatggat
    76081 gctgccgtca atgtttccgg catccacatc accaggccag agagcgtagg ccagttcgtc
    76141 atccagtgtt ttccatgtct gcttgtattc gcgatgaatg gcagcaatga ccgggctgat
    76201 ttttcctgtt gaattttcag tgtactgttg attggctctg gcgcgggcga gatcaacaac
    76261 agacgtgtat tttccggttt ccttgcgttc accttcgcga cgttttttcc agatgcgcat
    76321 ctctgcctga atttcgggcc atttagcacc aggaatacat ttatgcttaa cccacccaat
    76381 ggcgtgcaac ttaagctccg gatacatggc gttaacttct ggcattttca tcaacgcttc
    76441 aacgatatgt ccgtcgaatg ttgccatgtc ttcctgcaac aattcctgtg cgctaataac
    76501 catatcaacg gtgatgtttt cacatgtgtc gaacttaacc atgacagcgt tctgtacttc
    76561 aggggccagc ttgtcaaaag tgacgttcat cggatcggat tcagtctcaa ccgggacaaa
    76621 agaagcagac tcctcatccc agcggttttc ctgcatatat tcagcatccc atgaatcgag
    76681 ggcagggcgg ggtatgccag gtttatcctc gcagacaata aatttataaa cgcagtcctg
    76741 agcagccgga taatgttcca ggaattgcca gtgaaatttt gctcgagcac ggcgttcgtc
    76801 gccagcttca atggctgtgg ctacagccac agcgccttct tcccttgttg ccagttcgtc
    76861 aggaatagcg gcgcaaataa agactttact cattttgttt taacctcatg acagatttaa
    76921 ggatgaacaa atccctgcca ttgctggcat ataagaatga aaccggatat ttattacgga
    76981 actgttttaa agacctgccg ggatttcgat attatactgg tgaataactt tatcgaccgg
    77041 gtaacagtta ccgggaattt tctgttcggt tgctgcagtc atacactcct gcattgtcct
    77101 gtgaacactg actgcaatat caactggctc tccggaaaca agaaaaactg tcagaacaag
    77161 cgcaaatgct gaattcattg tgcacatcct tttggcatca gacgtaaacg agccagcatt
    77221 gaaacaatgc atattttatt taatagctcc cgttcttgtt ttctcttgtt aatggcatct
    77281 tcagtaaata cagggttact gatagtgaca ccaatttcaa aacaaccttc agacgtatta
    77341 acgtttggta ataacgtttt cattatcgcg tcctcaacaa tgaattttgt gatgcagtgc
    77401 ctggtgcctc caggtgacgt taaccagtta acaattaacg ccggatacag agaatccacc
    77461 cataacactg tttttggttt taactgttcc gcgtgcgctt agccgcattc accgcatcac
    77521 aaaattcact ttaaaaacgg cggcagagca gtcacggagt aaaactgata ccgccaaacg
    77581 tcaccagaaa attgataaca gagggcgttg cagcggggtt gtcacttaag cgtatggtca
    77641 acctgacaac tcggtgtcct caacggggaa ggaataaccc cgccatactt accgccgcgc
    77701 catttcgcgg gttgccacaa ccggaagcgc acggtcgaat taaatttaac gacaccgtac
    77761 agtgagacga acttcgccgt gcgctttcgt gttgtgtgcc tgcttttaac cacgtcaggc
    77821 gaggtggtat ccttaaaatc accacagttt taaggattca ttaagcaatg tcgcaaccac
    77881 caataaatcc gcttaagaac atgaaaattg attactggta taaagcgctt acagttgttg
    77941 gcgctgcgtt gtttgtcttt aatggaacgt ctttttttga cagatatccc gttgttccat
    78001 tgggtttttt gtcctccggc atctttttta ttggtttggg ggagtggatt aatcaccctc
    78061 tcaaagtgag atttattggt cctggagttt ggactcgtgg atataatcgt tcttcgtgcg
    78121 cactcggtat catcttcgac atacttggtt gtttcctgat tgttacagga gtcgtcaagt
    78181 tcttctgatg taaaaccgca aatggggcac gtaacgggaa ttttgaaaag cgtttctccg
    78241 ggttccagaa caaaattttc tgcggtctga ttttgcttct catatttgtg ctccgcgtca
    78301 ttgtgagagc acattcttat tctgagtgcc tgtttaaact cactgaagct gagagcttct
    78361 tcgccttcgg caaggccttc gaagtattct tcgtaagcct tttccatgat tgtgtcgaaa
    78421 tccatatcac tcacctgagt ttctttccag ccagcgacgg gcaccatttt cggttttaaa
    78481 cgttttgctt ttggtatacg tcattgcggt gaacgtgccg tcctggtttg gaaacacgcc
    78541 gtacaccaga gattcgttgt tgccaagatc gatagtatcc atgctgacct catttcccct
    78601 taacgccggg gtagcggaac tgtttgctga gaacaccgtg cggtgtcttg atggaaaata
    78661 atttagaata acctaacatg agaggcaagt gttttttgtt agattgatct aacaaaaagg
    78721 gtggacgcaa ctaatcactt gaaaagaatg ttattttatt gatttatttt tacgcgcttt
    78781 aagcatttct tcgaagagtt tgttgaagtt ttctactctt gcgcgcattt cagacagcaa
    78841 ggcttcctgc tcggaagatg gaagagcatc gaataattcg atcaattctt tgtggttggg
    78901 agttagctct gtttccacat gaagttcttg tgcaggcact ggtgccttgt cttcgtcacc
    78961 aaacattagc catgtaggtg agcacttcag agcatccgct aaagcaaaca atcgttttcc
    79021 gactggctgg gtttcgtctc tttcccattg tgaaattgtg acgtgagcaa ctccagcgag
    79081 gcgcgcggct tctcgttgtg ttaagcgtaa ttcttttcgt cgcgccagaa ctcgctggcc
    79141 cagggttctt gtatccatag ttaggtaatt ctaatttttc ttgacttagg tatcccgcgc
    79201 acaataatgt tagaaaagtc taacaagagg gggctttgat gcttaaagtt gacgcaatta
    79261 ctttttttgg cagcaaaaca aagcttgcca atgccgcagg agtgaggctg gcaagtgttg
    79321 ctgcttgggg gatactggtt cctgaaggtc gcgcgatgcg tctacaggag gcatctggcg
    79381 gggagcttca gtatgatccc aaagtttatg atgaatatcg taagacgaag cgggcggggc
    79441 ggttgaacaa tgaaaatcac tcctgaacag gctcgtgagg ctctggatgc ctggatatgt
    79501 cgaccaggaa tgacacagga gcaggcgacg atattaatca ctgaagcatt ctgggctttg
    79561 aaagagcgcc cgaacatcga tgttcagcgt gtcacagatg aaggtggcgc ggttgatcag
    79621 cgagcgcttg gcgttaatcg agtgaagata ttcgaacgct ggaaggctat cgacaccagg
    79681 gataagcgtg aaaagttcac ggcgctagtg cctgcgatta tggaggctat ccggattagt
    79741 gatttcaggt tgtatcgtga aattactgac ggaaaaagca tcacgtgcat gatcgccggg
    79801 ttaaacaaag aatatggcga tgtggtggag tccggactgc tttttgctga tcctgccgta
    79861 gtggatcgtg aaactgacga acttatagaa aaagcaattg ctttcaagct tgcgtatcga
    79921 cagcaatacc aacaaaaagc tggatggaat tatgagcctt ctttttgctg aacgcccact
    79981 ggttataaac acacagcttg cgatgaagat tggcttaaat gaagccattg tgttgcagca
    80041 gttgcattac tggttgagag ataccaattc cggcatggaa tgtgatggtg ttcgctggat
    80101 ttacaacaca acggaacaat ggctggaaca gttcccattc tggtcagagt caacgttaaa
    80161 gcgcgcgttt gcaagtctga aaacgctggg gcttttgcgt tgtgaaaagc tcaataaatc
    80221 aaagcgcgat atgaccaatt tctacacgat taactatggg agcgagcttt tagatgatgg
    80281 caaattgagc gaatccatcg gttcaaaatg cgccgctcca tcaggtcaaa atgacacgat
    80341 ggaagaggtc aaaatgaaac gctccattgg ttcaaaacga cccaatgtca tcgggtcaaa
    80401 atggcccgat gatcctacag agaatacaac agagattact acagagaata aaaacacttt
    80461 tcgtccggaa gcttcgcaac cggacccgca gacggctgaa caggattttt taatccggca
    80521 ccctggcgca gttgtgttta gtgcgaaaaa acgccagtgg ggtagccagg aggatctggc
    80581 gtgtgcgcag tggatatggg ggcggatcgt gggtctctac gaacaggccg ccagtgatga
    80641 tggcgagatc atgcgaccaa aagagcctaa ctggactgtc tgggccaatg atgtgcgcac
    80701 aatgcggatg ctggatggca gaagtcacag acaaatttgt gaaatgtttg gtcgggtaca
    80761 gcgggatcca ttctgggtaa aaaacatcat gagcccgtca aagctccgcg aaaaatggga
    80821 cgaactggtc atccgcctgg ggcgttcacc tgtacagcgt tgtgttaatc atatttctga
    80881 accggataca gaaattccgc ctggtttcag aggataagtt ttgatttcag gtcatgaggt
    80941 aattttaagg gggacttgtg gcaaaagttt ttacacaaga agagcgggaa aaaatcaagg
    81001 ggcaggttgt tgagctagta cgccggagtg ggcgcgagac gttacggcaa ctggaagcca
    81061 agacaggtgc gacaagatat ctgatgagcg ttctcgccag agagctggtt gccagtggtg
    81121 atgtatataa ctctggctac gggttattcc cgtctgaaca ggctcgtaag gactggcaaa
    81181 acgcccgcaa aaaactctcg agggcaaagg tgaagaaaac atctgtggtt gatccggacc
    81241 ttatctggtc attacctgac ggagaaatac gtcgttatga caggcaccaa aacataattt
    81301 gctgtgagtg ccggaagagc gaagttatgc agcgcatact ggcattttat cagtgaaatt
    81361 ttcgttattt atagacgtaa ctagattaaa gagcattagt tcagatgtga attgacattt
    81421 tcacggcaca ggattgagct agcgtggttg tctgctatgc gtcaaaagca gatattacca
    81481 gatttagaca tctattcccg atagccctgc tctgatgcca cactctgtgc tatttttatg
    81541 accccaataa aaatatttat gactattgct gatttcaaac ggcctaaatt ggagctccca
    81601 aacggggcaa acaaactact actgcactct tgctgtgctc catgttccgg tgaagtgatg
    81661 gaggcacttc aggcctcgag aatcgactat accatctttt tctacaaccc gaacattcat
    81721 cctcagaaag agtatttaat tcgtaaggat gagaatattc gctttgctga acaacacggc
    81781 gtgccattta ttgatgctga ttacgacaca gacaactggt ttgaacgtgc caaaggaatg
    81841 gaatgggagc ccgagagggg gatccgttgt accatgtgtt ttgacatgcg ttttgagcgg
    81901 acagcgctgt acgctgctga aaatggtttc agtgtgatca gcagttcact gggcatttca
    81961 cgctggaaaa atatgcagca ggttaacgac tgtgggcggc gagccgtcgc gcattatccg
    82021 ggcatggtgt actgggatta taactggcgc aagcagggcg gctcgtcccg catgattgaa
    82081 atcagcaagc gcgaaaaatt ctatcagcag gaatattgtg gctgtgtgta ttctctgcgc
    82141 gataccaatc tacaccgcaa atctcaggga cgccctctta tcaaaattgg tcaactccac
    82201 tacgataaag aagagaagga gtgattttat ggagcacctt tcttattgat ttcatattgg
    82261 cgaggtagcg ggagttaagt aaaattgctg cgggtgcttg aggttgtctg tctcaggcat
    82321 ccgcagaaaa atcagcaaaa tgctcgtttt atgcacaatt gtaggatggt aatctgatgc
    82381 ataattattc gaaataacat aaaatactag acgtgtaggc tgtggggtag ttatgagagt
    82441 aaataaacta catttaggtg ctagagtttc gctatttata gtgtcatacc ttcccctatt
    82501 ctttatcatg tgttttgtgc aattgtatac ttatagaagc tatttaaatt ggaaaggtat
    82561 aagtgttgaa tcattaacta ttttctttaa atacttt
//
LOCUS       DAPOZO010000005        79321 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPOZO010000005 DAPOZO010000000
VERSION     DAPOZO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.005"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(39..1124)
                     /locus_tag="ROI21_000374"
     CDS             complement(39..1124)
                     /locus_tag="ROI21_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDX5109350.1"
                     /translation="MKTYPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTRGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIAQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(1136..2440)
                     /locus_tag="ROI21_000375"
     CDS             complement(1136..2440)
                     /locus_tag="ROI21_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDX5109351.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWFGLFVPA"
     gene            complement(2452..2805)
                     /locus_tag="ROI21_000376"
     CDS             complement(2452..2805)
                     /locus_tag="ROI21_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDX5109352.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(2816..3694)
                     /locus_tag="ROI21_000377"
     CDS             complement(2816..3694)
                     /locus_tag="ROI21_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDX5109353.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYGFICQEMNDLM
                     AQGVRNVIEMTNRYMGRNAQFMLDVMHETGINVVACTGYYQDAFFPQHVATRSVQELA
                     QEMVNEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DERRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(3691..4917)
                     /locus_tag="ROI21_000378"
     CDS             complement(3691..4917)
                     /locus_tag="ROI21_000378"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDX5109354.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPMLEKMGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQVLVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDDKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(4918..6081)
                     /locus_tag="ROI21_000379"
     CDS             complement(4918..6081)
                     /locus_tag="ROI21_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDX5109355.1"
                     /translation="MFVEALKRQNPALISAALSLWQKGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSMGA"
     gene            complement(6165..6527)
                     /locus_tag="ROI21_000380"
     CDS             complement(6165..6527)
                     /locus_tag="ROI21_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDX5109356.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGMM
                     AMTHMASALMRSRRGEEIEPLDAELLAELAQSSHWQAVVQLHQVLLKEFTLEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6544..7449)
                     /locus_tag="ROI21_000381"
     CDS             complement(6544..7449)
                     /locus_tag="ROI21_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109357.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDSRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKQALLAHQQRVVSGEQEPSY"
     gene            complement(7609..8613)
                     /gene="trpS"
                     /locus_tag="ROI21_000382"
     CDS             complement(7609..8613)
                     /gene="trpS"
                     /locus_tag="ROI21_000382"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDX5109358.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHLFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8606..9364)
                     /gene="gph"
                     /locus_tag="ROI21_000383"
     CDS             complement(8606..9364)
                     /gene="gph"
                     /locus_tag="ROI21_000383"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDX5109359.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLATAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINNLLPALGLPHSENQESKND"
     gene            complement(9357..10034)
                     /gene="rpe"
                     /locus_tag="ROI21_000384"
     CDS             complement(9357..10034)
                     /gene="rpe"
                     /locus_tag="ROI21_000384"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDX5109360.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10052..10888)
                     /gene="dam"
                     /locus_tag="ROI21_000385"
     CDS             complement(10052..10888)
                     /gene="dam"
                     /locus_tag="ROI21_000385"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDX5109361.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(10995..12281)
                     /gene="damX"
                     /locus_tag="ROI21_000386"
     CDS             complement(10995..12281)
                     /gene="damX"
                     /locus_tag="ROI21_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDX5109362.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAEHPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12373..13461)
                     /gene="aroB"
                     /locus_tag="ROI21_000387"
     CDS             complement(12373..13461)
                     /gene="aroB"
                     /locus_tag="ROI21_000387"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDX5109363.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LVPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13518..14039)
                     /gene="aroK"
                     /locus_tag="ROI21_000388"
     CDS             complement(13518..14039)
                     /gene="aroK"
                     /locus_tag="ROI21_000388"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDX5109364.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14440..15678)
                     /gene="hofQ"
                     /locus_tag="ROI21_000389"
     CDS             complement(14440..15678)
                     /gene="hofQ"
                     /locus_tag="ROI21_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDX5109365.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15590..15994)
                     /gene="hofP"
                     /locus_tag="ROI21_000390"
     CDS             complement(15590..15994)
                     /gene="hofP"
                     /locus_tag="ROI21_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDX5109366.1"
                     /translation="MRGKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(15984..16424)
                     /gene="hofO"
                     /locus_tag="ROI21_000391"
     CDS             complement(15984..16424)
                     /gene="hofO"
                     /locus_tag="ROI21_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDX5109367.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHQEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWRPSAQGGELALK
                     TLWEAVPLAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16408..16947)
                     /gene="hofN"
                     /locus_tag="ROI21_000392"
     CDS             complement(16408..16947)
                     /gene="hofN"
                     /locus_tag="ROI21_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDX5109368.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVDTVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQLQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(16947..17726)
                     /gene="hofM"
                     /locus_tag="ROI21_000393"
     CDS             complement(16947..17726)
                     /gene="hofM"
                     /locus_tag="ROI21_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDX5109369.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGTKECFLQRWWRLPLENDII
                     KDGRIVDAQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGITSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            17846..20398
                     /gene="mrcA"
                     /locus_tag="ROI21_000394"
     CDS             17846..20398
                     /gene="mrcA"
                     /locus_tag="ROI21_000394"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDX5109370.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20566..21126)
                     /gene="nudE"
                     /locus_tag="ROI21_000395"
     CDS             complement(20566..21126)
                     /gene="nudE"
                     /locus_tag="ROI21_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDX5109371.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            21446..23581
                     /gene="igaA"
                     /locus_tag="ROI21_000396"
     CDS             21446..23581
                     /gene="igaA"
                     /locus_tag="ROI21_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDX5109372.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEEHSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTNSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            23646..24314
                     /gene="yrfG"
                     /locus_tag="ROI21_000397"
     CDS             23646..24314
                     /gene="yrfG"
                     /locus_tag="ROI21_000397"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDX5109373.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAREYMRQQYHDVQHTLNWYCFDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24325..24726
                     /gene="hslR"
                     /locus_tag="ROI21_000398"
     CDS             24325..24726
                     /gene="hslR"
                     /locus_tag="ROI21_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDX5109374.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            24751..25629
                     /gene="hslO"
                     /locus_tag="ROI21_000399"
     CDS             24751..25629
                     /gene="hslO"
                     /locus_tag="ROI21_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDX5109375.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNKQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(25692..27416)
                     /locus_tag="ROI21_000400"
     CDS             complement(25692..27416)
                     /locus_tag="ROI21_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDX5109376.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSNRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLLTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKSSL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIGKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            27795..29417
                     /gene="pckA"
                     /locus_tag="ROI21_000401"
     CDS             27795..29417
                     /gene="pckA"
                     /locus_tag="ROI21_000401"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDX5109377.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTRILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            29534..29851
                     /locus_tag="ROI21_000402"
     CDS             29534..29851
                     /locus_tag="ROI21_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDX5109378.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            29910..30206
                     /locus_tag="ROI21_000403"
     CDS             29910..30206
                     /locus_tag="ROI21_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5109379.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEEAPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(30238..31590)
                     /gene="envZ"
                     /locus_tag="ROI21_000404"
     CDS             complement(30238..31590)
                     /gene="envZ"
                     /locus_tag="ROI21_000404"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDX5109380.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGATKEG
                     "
     gene            complement(31587..32306)
                     /gene="ompR"
                     /locus_tag="ROI21_000405"
     CDS             complement(31587..32306)
                     /gene="ompR"
                     /locus_tag="ROI21_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDX5109381.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32534..33010
                     /gene="greB"
                     /locus_tag="ROI21_000406"
     CDS             32534..33010
                     /gene="greB"
                     /locus_tag="ROI21_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDX5109382.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            33107..35428
                     /locus_tag="ROI21_000407"
     CDS             33107..35428
                     /locus_tag="ROI21_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDX5109383.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            35868..36095
                     /gene="feoA"
                     /locus_tag="ROI21_000408"
     CDS             35868..36095
                     /gene="feoA"
                     /locus_tag="ROI21_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDX5109384.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            36112..38433
                     /gene="feoB"
                     /locus_tag="ROI21_000409"
     CDS             36112..38433
                     /gene="feoB"
                     /locus_tag="ROI21_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDX5109385.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAANTTGDCH"
     gene            38433..38669
                     /gene="feoC"
                     /locus_tag="ROI21_000410"
     CDS             38433..38669
                     /gene="feoC"
                     /locus_tag="ROI21_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDX5109386.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGNCKSCPEGKACLREWWALR"
     gene            38872..39765
                     /gene="rpnA"
                     /locus_tag="ROI21_000411"
     CDS             38872..39765
                     /gene="rpnA"
                     /locus_tag="ROI21_000411"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDX5109387.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPNLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGFQKGIRTNALNIAKTMLDAGVS
                     LEDVLRFTSLTVEDLAEINHQ"
     gene            complement(39985..40755)
                     /gene="bioH"
                     /locus_tag="ROI21_000412"
     CDS             complement(39985..40755)
                     /gene="bioH"
                     /locus_tag="ROI21_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDX5109388.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQSLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            40793..41476
                     /gene="gntX"
                     /locus_tag="ROI21_000413"
     CDS             40793..41476
                     /gene="gntX"
                     /locus_tag="ROI21_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDX5109389.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THTHLPCGRCLHKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            41535..42110
                     /gene="nfuA"
                     /locus_tag="ROI21_000414"
     CDS             41535..42110
                     /gene="nfuA"
                     /locus_tag="ROI21_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDX5109390.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            42471..43787
                     /gene="gntT"
                     /locus_tag="ROI21_000415"
     CDS             42471..43787
                     /gene="gntT"
                     /locus_tag="ROI21_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDX5109391.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(43835..45916)
                     /gene="malQ"
                     /locus_tag="ROI21_000416"
     CDS             complement(43835..45916)
                     /gene="malQ"
                     /locus_tag="ROI21_000416"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDX5109392.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLEDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFADCWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRSPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKK"
     gene            complement(45926..48319)
                     /gene="malP"
                     /locus_tag="ROI21_000417"
     CDS             complement(45926..48319)
                     /gene="malP"
                     /locus_tag="ROI21_000417"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDX5109393.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQAMHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            48942..51647
                     /gene="malT"
                     /locus_tag="ROI21_000418"
     CDS             48942..51647
                     /gene="malT"
                     /locus_tag="ROI21_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDX5109394.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(51690..52706)
                     /gene="rtcA"
                     /locus_tag="ROI21_000419"
     CDS             complement(51690..52706)
                     /gene="rtcA"
                     /locus_tag="ROI21_000419"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDX5109395.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVHGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFICRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVAAEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLVASFSL
                     HEQNIHSLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(52710..53936)
                     /gene="rtcB"
                     /locus_tag="ROI21_000420"
     CDS             complement(52710..53936)
                     /gene="rtcB"
                     /locus_tag="ROI21_000420"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDX5109396.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWTELEVGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            54124..55722
                     /gene="rtcR"
                     /locus_tag="ROI21_000421"
     CDS             54124..55722
                     /gene="rtcR"
                     /locus_tag="ROI21_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDX5109397.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSSETEVVGVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLLQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITREVVEDEINRLRYNWQES
                     RPSAITALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(55704..56462)
                     /locus_tag="ROI21_000422"
     CDS             complement(55704..56462)
                     /locus_tag="ROI21_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDX5109398.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(56479..57309)
                     /gene="glpG"
                     /locus_tag="ROI21_000423"
     CDS             complement(56479..57309)
                     /gene="glpG"
                     /locus_tag="ROI21_000423"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDX5109399.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELVRFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(57354..57680)
                     /gene="glpE"
                     /locus_tag="ROI21_000424"
     CDS             complement(57354..57680)
                     /gene="glpE"
                     /locus_tag="ROI21_000424"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDX5109400.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            57870..59375
                     /gene="glpD"
                     /locus_tag="ROI21_000425"
     CDS             57870..59375
                     /gene="glpD"
                     /locus_tag="ROI21_000425"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDX5109401.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGTNSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAIGDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(59428..60033)
                     /locus_tag="ROI21_000426"
                     /pseudo
     CDS             complement(59428..60033)
                     /locus_tag="ROI21_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(60036..60803)
                     /locus_tag="ROI21_000427"
     CDS             complement(60036..60803)
                     /locus_tag="ROI21_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109402.1"
                     /translation="MQPSPAMQAVIEQVYHIFRRYPVPQKFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDERAILQQFACQYMTDWVSGDEIVELQYMLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITTQTNTWASNNQSILKERARQAIEN
                     PLNQPEQGTHYQVWEDDWMIDECLCAMYDASSESPGK"
     gene            complement(60906..62414)
                     /locus_tag="ROI21_000428"
                     /pseudo
     CDS             complement(60906..62414)
                     /locus_tag="ROI21_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
     gene            complement(62539..64986)
                     /gene="glgP"
                     /locus_tag="ROI21_000429"
     CDS             complement(62539..64986)
                     /gene="glgP"
                     /locus_tag="ROI21_000429"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDX5109403.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPELMRL
                     LIDEHQFSWDDAFGVCCEVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDKSNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(65005..66438)
                     /gene="glgA"
                     /locus_tag="ROI21_000430"
     CDS             complement(65005..66438)
                     /gene="glgA"
                     /locus_tag="ROI21_000430"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDX5109404.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(66438..67733)
                     /gene="glgC"
                     /locus_tag="ROI21_000431"
     CDS             complement(66438..67733)
                     /gene="glgC"
                     /locus_tag="ROI21_000431"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDX5109405.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(67751..69724)
                     /gene="glgX"
                     /locus_tag="ROI21_000432"
     CDS             complement(67751..69724)
                     /gene="glgX"
                     /locus_tag="ROI21_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDX5109406.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCIFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(69721..71907)
                     /gene="glgB"
                     /locus_tag="ROI21_000433"
     CDS             complement(69721..71907)
                     /gene="glgB"
                     /locus_tag="ROI21_000433"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDX5109407.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(72180..73283)
                     /gene="asd"
                     /locus_tag="ROI21_000434"
     CDS             complement(72180..73283)
                     /gene="asd"
                     /locus_tag="ROI21_000434"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDX5109408.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            73475..74068
                     /gene="yhgN"
                     /locus_tag="ROI21_000435"
     CDS             73475..74068
                     /gene="yhgN"
                     /locus_tag="ROI21_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDX5109409.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(74125..75465)
                     /gene="gntU"
                     /locus_tag="ROI21_000436"
     CDS             complement(74125..75465)
                     /gene="gntU"
                     /locus_tag="ROI21_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDX5109410.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(75469..75996)
                     /gene="gntK"
                     /locus_tag="ROI21_000437"
     CDS             complement(75469..75996)
                     /gene="gntK"
                     /locus_tag="ROI21_000437"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDX5109411.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(76135..77130)
                     /gene="gntR"
                     /locus_tag="ROI21_000438"
     CDS             complement(76135..77130)
                     /gene="gntR"
                     /locus_tag="ROI21_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDX5109412.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(77354..78049)
                     /gene="yhhW"
                     /locus_tag="ROI21_000439"
     CDS             complement(77354..78049)
                     /gene="yhhW"
                     /locus_tag="ROI21_000439"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDX5109413.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(78172..79209)
                     /locus_tag="ROI21_000440"
     CDS             complement(78172..79209)
                     /locus_tag="ROI21_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDX5109414.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
BASE COUNT        19246 a        21662 c        19832 g        18581 t
ORIGIN      
        1 ttatttctgc attcttttcg aaaacatcat gcacaagatt aaatactggc aatactctca
       61 cgcaaaatcc gcagcaccgt ctcttcaccg ctgcgatttg ggttaatgcg aatcgcgcaa
      121 tgttcgagtt gcggattcgc ctggcgaaac gttccggaaa gacggtaaaa gagcggcggg
      181 atttcatatt tcgactctgc accgaccggg taaggcaagg caccgagctt ttgcgcctct
      241 tccagtactc tggcggcaat cggctgatga aactccacaa tcaacacctt cgactgcgca
      301 ttggcaatga cggcgctttt cacttccgga actgcaccac cgttgagcaa ggccagcaac
      361 cgttcagata cccccgcctg caccgcgtgc atcactggcg caaacaccag cccacgcaag
      421 acttccagcg cctgagcgcc ctgaatctgg ctgccgccgg aatagagcgt ggcgcgaatc
      481 cgactaataa catcggcatc acctaccacc gcgccaacac cctccggccc aaacagcttg
      541 aagcaggaaa aagtcgagac atttgctccg cattcgcagc caattcgcgc cactttcatc
      601 accgcatagt tgtcatcggt taatgcagga acgccagccg agcgcaacgt tgccagcaca
      661 tctgccagaa tgtacccgtc ctgcggctgc tggcgcgtat gctgcaccag cgcggcatct
      721 ggttgttgct cgtcgacaac ctgcttcagt gccaacaggt cattgaaatc agcagtgata
      781 agcgtcagcc ccatctgcgc aataatgacc tgagtcgtcg ggtaaacagg cgcgtcatgc
      841 accagaagac gctgccccgg tttgagcaaa gccgccaacg ccgcacgaat cgctccagta
      901 cccgcaccct gtaccagcgc cgcagcctgt gcgtgaaatg catcagccag cacctgttcc
      961 actcgctggg taatgcgcgg ttgattcagc cccggcgtta agcccaaatc cccgcgggta
     1021 agaaactcgc tgccgggaaa atggcggcaa atcgtgtcca ccagcgcaaa ctgcttttgc
     1081 tgcgcctcaa taagcgtcag gctttgcaaa ggatacgtct tcataggtgc cttccttaag
     1141 ccggaacgaa caggccaaac cagtaaagaa cattcagcag aataccggtg atcattactg
     1201 ccacgaccgg tgccgccatt ttctgtaccg gacggcccag ggattcgttg aggaagtaaa
     1261 tggcaactgc gatagaaaat ccggtataac ccgccatttt aattgctgcg aaaatcgaac
     1321 cgaccagcaa cgccacttcc atcagcatat tcatggcgtt acggatgtta tctgacgcat
     1381 tacgcaccga cggatagcgc cccagccatt tgccgatcga acgaagcagc aggacttccg
     1441 ccgaaataac cactgcgcct aataccgctg caaccatcgg attcggcgag agatagccca
     1501 ccgcataaac aaaggtaaag cccgcaactg catacacacc agtggctaac gcggtggtgg
     1561 caatcaacgg tacaaaaccc agcccgcgca taaattccgc cagagcggcc tggttaatca
     1621 gcgtttgtga ttgttccggc gttacgcctg cggaatatgc tttctccagt gtgaagatcg
     1681 acacttcact gccagcaaaa atcttcatgc tggcaacggc ggcaatcagc gccccgacaa
     1741 tagcgatata cggtaagttt ttgataatcc gcgacgtgcg ttcttcaaac accgaaagcc
     1801 cgctggcatc aatatcattt tcatcacgat gacgcaggtc gtgagttatc gcgatcccca
     1861 gcagcatcac catgccaata aagatttcga tggattccgg attgagatgc gggaaatagc
     1921 gcactaccac cacacgggtc atcagtacca ccacggcggc gatcagactt tgcttccagc
     1981 caaactggta gaaaatcgcc accaacggga acagtgcaaa agctgaaacc accggcgagc
     2041 ttaattcccc caggctacct aatacatcca ccggtagcgc ggtcagcagc tggtttaccg
     2101 gtaacaggca agtaaggatc aacacgcccc agatagcgcc aagaccaaac gccatcaggc
     2161 tgtttatcgc cagtacgccg aggatatcgg taggaagaaa gagtaaccat gcgttgagca
     2221 gtccggtttt cagggtaaaa gagatcccca ccgaagccac aaaaccaatg ctcagaccaa
     2281 aagcgatact ccccgcctcg cgacggttca tatagccttc aatcagttgc ggcaggatcg
     2341 ggcggatacc gtcatgaaaa acggccgccg agcgatgcgc cagaagcgat gtcatacccg
     2401 tcaggcacgc caccacgata atctgaatat acagatccat agtcgtgcct tttatttcag
     2461 atggttaatc agcatcggaa tcgcgtgttc gacgtgctca acggaaagcc caaacgccac
     2521 tttcccttcg gcgatcattt tggcgatatg ttcgtctttc gctttaatgc ctggtttggc
     2581 aatggtgcaa cttttgttat agccaatcac cgcgatggca atcgacaatg ccgcgcccgc
     2641 accggtatta cacgcgccga tgtagtaatc cagttgcccg gatttgactt tcattgccgc
     2701 ttccatgtcg ttgtgaatga aaacttcaaa acagccagga gccgtcgctt caatggtttt
     2761 tttaatctgc tcacgctgta agcctgcaac accaatcttt ttcatgagtc tgtccttatt
     2821 ggaaaaattg agagggagtt tcacgtaaca tcacatccac atcggcctga ctgaatcctg
     2881 actggcgcaa ttgcggaata aaggtggtca ataaatagtc atagccataa ccaccgttgg
     2941 cttttaaatg ggagcggcgc gtaatatcca tcgacagcat gacgcggttc agcaaaccac
     3001 ggtcacgtag cgcgtggagc atcgcaatgc gcctttcgtc cgggtagtaa ctgttcttgc
     3061 cgatggtgtc gaactgcaca tacgcgccga gatcgatcat cttcaaaatg ttgtcgaggt
     3121 tgtctttcag atcgcagtga ccaacggtga cgcgcgaaag atcaactccg tgggcttgca
     3181 acagagccag ttgttccagc cccatcgtgc tgaacgacgt atgcgtggag atcgggcgtc
     3241 cggtctggtt atgtgccagc gcggcggcaa taaacacctt ctcttccagc ggcgtaatct
     3301 tcccttcgct ggagccgatc tccgcgataa tcccggcttt cagctccgtg ccatcgatac
     3361 cctgctcaat ttcattgacc atctcttgcg ccagttcctg cacactgcgg gtcgccacat
     3421 gttgcgggaa aaacgcgtcc tggtaataac cggtgcaggc caccacgttg atccccgtct
     3481 cgtgcattac atcgagcata aattgcgcat tgcgccccat ataacggttg gtcatctcaa
     3541 tcacattacg cacgccctgg gccatcaggt cgttcatctc ctggcaaatg aacccatact
     3601 gatcaaggcg gcagtccacg ttgtttttaa agccggagag atcaatatgc agatgctcat
     3661 gggcgagggt gtagcccgtc ggatcaaaac tcatagggtg tctcctgcaa accggaggga
     3721 ggaaagaaaa gagcgaccat tttgtggcgg tggcgcacgg aaaaattcac acacggtagc
     3781 ccccacatca gaaagcgtgg tgcgcacacc aagctgcgta gcgaccagcc cttgctgata
     3841 aaccagcact ggcaccactt cacgggtatg gtggctgtga ccaatggtcg gatcgttacc
     3901 gtgatccgcc atcacgacca ggcaatcatc tggctgcatc gcctcaataa gccgggcaag
     3961 gttacggtca acgacctgca aacgttcggc ataacgtgcg acgtcttccg cgtgaccagc
     4021 gaggtcggtt tcctggatat tggtgcaaat aaacgccgtc ggatgcgtgt taaattcgtc
     4081 gagggtgata tccataatcc gctggctatc caccaggttt tgccagctca cgccataagg
     4141 attactggca atatccgcca ctttccccac cagcacggtt ggtacgccca cttcatacag
     4201 tttttgtggc acctgcactt tatcatcgac accgtagccc atatgcacga cctggaagcc
     4261 gttgtcataa gcgcctgaac gcggcgcatt gataccaata aagcgccctt ctttactttc
     4321 tgcggcatcg agaatgcgtt gactgtcagt taacaggccg ccaaatgtaa tgacccgacc
     4381 gacctgtacc tggtcacgca cgatacgacc aattttgatt gcgtcgtcaa aagagatcac
     4441 agagagattg gcggtaatgt tatagacctg gcctaaatcc gcctcgagat tatcgccaat
     4501 cgcaaccgcc tgattgaccc acagaaattg cagatcatcg ccacggcgct ccacctgcca
     4561 accggcggat actaatacct gctcaacacg gtcaatcaca tcgcgaaagg gcatccgcag
     4621 cggcggtaac gggcgcgtgc ctaaaatttc ctgatgcccc ataaaggtat cgccaccttc
     4681 atgttgcagc tctgccacgc cccaggttgc ggagtctgac ggctgcatat ctccaggcgc
     4741 ataacctaat gcgttgatta gccccatttt ctccagcatt ggtagctgca aatgcggcag
     4801 ctggctcagg atgtgaccac aggtatttgc cccggcatcc tgcggacgca ccaacgtgac
     4861 atctttcatt gcccctacgc caaagctatc aatcactaac accacaaatc gcgccattta
     4921 cgcccccata gagtttccaa gactgtcata acgaccgacg atttctggtt cgccacggtg
     4981 aataccggac agcaacacca catcgctacg ggtgacaaaa atctgcgtgc gaaaacagag
     5041 cactactgca ctgcttaccg gaaactcgcc tgccagcggc agggtgtagt cgatactgct
     5101 gtcatccaca gttttgagat tggtttcagt aatcttttga ttttctggcg taaacaccag
     5161 cgcatgttgc gcgtgaccac gacggtaata accgccgccg tagcagtagc tgtcgccacg
     5221 gaaatgatgg gagatttcac ttaaccagag catcgcgata cgttcaggct ggtcgccctg
     5281 ctggtttgcc ggaatcgtcc ccgtcagcgc atgaccgggt tcggcatgag tcacaccgta
     5341 ttccgccagc aatggcagcg aagtgcagct ggtcgcagaa ggtgcgttca gttgctcaat
     5401 cgcaatacca gatttcgcta gttgatcccg tgcctgtatc agcgtgtgaa gattcggtgt
     5461 cggcaaaact ttcccggcag cctcatccca aagcaggcaa gggaaatggg ttagtccagc
     5521 taaatgcaac cccggcagtt tcttaatttc agcaaccacc tcgtgcagcg aatgctgtac
     5581 aaaaccgctc tcctgccccg gataaagaaa atcatcgtcg ctataaactt tgagcagcac
     5641 agactgcaca cgtccggcct tcaccgctgc cgcagaaact tcccgcgctt tgtcgagagt
     5701 aaacacggtg atgacgtcgg tgccctgtgc aacggcgtca gcaacctgat gacaagggat
     5761 ttgtaccaaa tgcccctgat gcgccaccgg caaaccggcg cggcgcatga ctcgcgcctc
     5821 tttgtaatcc accgccacaa taccgctgta gcccagcgcc agcagttttt ccgccagcca
     5881 aggattgcga ccgaactgct tggtcatcag atacagttca atgccgtaaa gccgcgccgt
     5941 ctcaatcagc cgcttaccgt tttccagtat ctgatccacg tcgatcaccc agctgtccgg
     6001 ggcgatcttg cccttttgcc acaagcttag tgcggcggaa attagcgccg ggttctggcg
     6061 tttcaatgct tctacaaaca tcttgttgcc tcgtttctgt tccttgaatt aaatattcat
     6121 ttttttgaat atttaggttg tgcgacgtgg atttgtcggg agcttcaaac ctcttcgtta
     6181 gcagccatcc ataatccata aaggttggcc agcaaatagc cttcttcaca cgggttaact
     6241 tccagtgtaa attccttcaa caacacctga tgcaattgca caacggcttg ccagtggctg
     6301 gattgcgcca gttctgccag caattcggcg tccagcggct ctatttcttc accacggcga
     6361 ctgcgcatca gtgcgctcgc catatgtgtc atcgccatca ttccttgctc gctgcgcacc
     6421 ggtagatgac actctgtttc cagtacgttg acgacctgca tcatgccttt gcagatgtcc
     6481 ttatcaataa cgcctgcatc gcaaagcagg ttgagtctgg tttccatttc agatgccttt
     6541 cgtttaataa ctgggttcct gttccccact gaccactcgt tgttgatggg caagcagggc
     6601 ctgtttatca acaactgcac gcagcaattg ttgcattggg taatcatcga ctcgcgtcaa
     6661 aaccacggct tcggtggcct gtaaaaatcg cgaatcgtct gtgagcggcg tcgcttctaa
     6721 tcccagcatg gtcagttcgt tttccgccac cacgttccag atcaccgcat cgacatcgcc
     6781 tttaacaatg cgttgtaaac tctcgtgata agagagatcg acacgttcca catcactatc
     6841 gccaaaaaag acatcggtca tgattttctg atccgccgaa cggttatcca gccccacgcg
     6901 cttcacgttt gcggactcgc ctttacggca aatcaactgg tgctcgccaa cgtaggtgtg
     6961 cggccccaac tccagcgcga ggcataagcc tttttgcgtg agataacttt ccgccgccag
     7021 tcgggaaaca accgccatgt catacacgcc gttaagtaaa cactccacgc gaatatccgc
     7081 gccgcgcata tgcgcatagt aaaaaggaat accatcaaac tgagctttca atccgctcgc
     7141 caggccttcg tacaaacggg tatagggcaa gggcatcgca cataccacgt tgttgatatc
     7201 cacatgagtc agcaatgctt tgttatccat ctcgaccaga taactgccat tgcgcccacg
     7261 gcgttcaatc cgtatcgctc cgcttgattc cagcgttttc aatgcggcct gcgtcaggcc
     7321 aacggatgaa cggcattcag ttgccagctc atctatggtt ttcagtcgat taccgcactt
     7381 ttcacccaac aaataacggg ccagtgtcgt gataacgaca ccttcttttt tgataaacgt
     7441 tcgacgcata gtaaattttc aataaagtga atatttatat cttcataaaa atgaagataa
     7501 gtggcaactg cgagatgtgg aaacggcgag gcgcttcaca ttttttcgga ttattgcaaa
     7561 tgaggataaa aaaaccgggt ttccccggtt tttggcatta atgttaaatt acggcttcgc
     7621 cacaaaacca atcgcttcgt acaccgcttt tagcgtacgg gaagcgtgcg cgctggcttt
     7681 ttccgcgcca tctttcatca cctgttgcag gaaggcttca tcgttgcgga aaaggtgata
     7741 acgttcctgc aattcagtca gcataccgga aacggcatca gccacttcgc ctttcagatg
     7801 accatacatc ttgccttcga actgtttttc cagttccggg atgctctggc ccgttaccgc
     7861 agaaaggata tccagcaggt tggaaacgcc cgctttgttc tgcacatcgt agcgaactac
     7921 cggcggctcg tcggagtcgg tgaccgcgcg tttgattttc ttcactaccg atttcggatc
     7981 ttccagcagg ccgataacgt tattgcggtt atcgtcagac ttggacatct ttttggtcgg
     8041 ctccagcagc gacattacgc gcgcgccaga tttcggaata aacggctccg gcactttaaa
     8101 gatctcgcca tacagcgcgt tgaaacgctg ggcgatatcg cggctcagtt ccaggtgctg
     8161 tttctggtct tcacccaccg gtaccagatt agtttgatac agcaggatgt ccgcagccat
     8221 cagcaccgga tagtcaaaca gaccagcatt gatgttctcg gcataacgcg cagatttatc
     8281 tttaaactgc gtcatgcggc tcagttcgcc gaagtaggta tagcagttca gtgcccagcc
     8341 taactgcgca tgttccggca cgtgggactg aacaaaaatg gtgcttttct caggatcgat
     8401 accacaagcc agatacaagg ccagcgtatc cagcgtcgct ttacgcagct tctgtgcatc
     8461 ctggcgcacg gtgatcgcgt gttggtcaac gatacagtaa atgcaatggt agtcatcctg
     8521 catgtttacc cactgacgca gcgcacccat gtagttacca atggtcaatt cacctgaggg
     8581 ctgtgcgcca ctaaaaacga tgggcttagt catttttcga ttcctgattt tcgctatgcg
     8641 gaagccctaa tgcgggcaga aggttattta tagactgata aattacatca ggctggctga
     8701 gatcgatagc ctcgccgtag ttatatccgt aggttaagcc aactgatggg caacctgccg
     8761 cttttgctgc ctgaatatca ttgcgtgagt cgccaacaaa cagcatctgt tgtggagcaa
     8821 ttcccatccg ctcagccacc agtaacagcg ggtccggatg cggttttttg ttttgcacat
     8881 catcaccacc aatcaccacg ctgaagtatt tggcgatatc taaggcttcg agcagcggcg
     8941 cgacgaacgg cgtcggtttg ttggtgacca ggcctagcgg caggccttta gcctgcaatg
     9001 cgcccagcgt atcggcaacg tgcgggaaca aaaacgtccc ctcttcggca acctcgccat
     9061 agtagcgatc aaacagttta cgcagaatac gtacctgttc ttctgccgga atgtcgtcat
     9121 caacgggcgg tttacccatt gttttacgca gagtcgcacg ttcctgacgc gcccaggtca
     9181 atgcgcgctc catcagaata tctgcgccgt taccaatcca ggtaataacg cgttcttcac
     9241 ctgcgacagg caactccagc gcatacagcg ccatatctac tgcggtagcc aagccaggag
     9301 cactgtccac cagcgtaccg tcgagatcaa aagcgacgcc gcgaatatct tcaaacttac
     9361 tcatgactta cctttgccag ttcactgcgc atttcatcaa tgactttttt gtagtctggc
     9421 tggtcgaaga ttgccgaacc ggcaacgaac atatccgcgc ccgccgcagc gatttcgcca
     9481 atgttgttca ccttcacgcc accgtccact tccagtcgaa tgtcaaagcc agactcgtcg
     9541 atacgacgac gtacttcgcg cagtttatcc agtgtttgcg gaatgaaaga ctgaccgccg
     9601 aaacctgggt tgacggacat cagcaggatc acatccagct tatccatcac gtaatccaga
     9661 tagctcagag gtgtcgccgg gttaaatacc agacccgctt tacagccatt ttctttaatc
     9721 agttgcagcg tgcgatcaac atgctcggag gcttctggat gaaaggtaat gatgctggca
     9781 ccagcggcag cgaaatcagg cacaatgcga tcgacgggtt tcaccatcag atgtacgtcg
     9841 ataggggcgg taatgccgta gttacgcaag gatttcagca ccattggccc aatcgtcaga
     9901 ttgggaacat agtggttatc catgacgtca aaatgcacga catcagcgcc agctgccagg
     9961 gcttttgcgg tatcttcacc caggcgggca aaatcagccg acagaattga gggggcaatc
    10021 aaatactgtt tcatccgctt ctccttgaga attatttttt cgcgggtgaa acgactcctg
    10081 gtttgtacaa agccagcagt tcgtccacct ttttacgtgt gccgccgttg ctgcttatac
    10141 tgcgtcgaac tttgacgaca tgcaattttg cgcgctgata ccactcacgc gttaacatcg
    10201 tatcgtgatt ggagatcagc actggaatat ggcgatcaac cagaccttca gcgatctccg
    10261 ccagatgcgc ttgttgttca agcgtaaaac tgtttgtgtg atacgccgta aagttggcgg
    10321 tcgcagacag cggcgcataa ggcggatcgc aatagacgac ggatgcatca tctgcgcgcg
    10381 ccatgctatc ggcgtaagac tcacaataga aaaaggcatt ctgcgctttt tcagcgaagt
    10441 gatacaactc tgcttccggg aaatagggtt ttttgtagcg gccaaacggc acattaaact
    10501 caccgcgcag attgtaacga cacaggccgt tgtaaccgta gcggttcaaa tataaaaaca
    10561 gtaccgcccg acggaacgga tcctggcttt tgttgaactc ttcgcggaac tgatagtaaa
    10621 cctcggcgca atttgtttcg ggaacaaaca gctcgcgtgc ggcctgtacg tactcatcag
    10681 tacgcatctt cacaatgtta tagagactga tcaggtcgct attgatatcg gcaaggatat
    10741 aacgagaaaa gtcggtgttg agaaacaccg acccggcacc tacaaaaggc tcaaccagac
    10801 attcgccctt gggcaaatgc cgtttaatat catcaagcag gggatacttg ccccctgccc
    10861 acttcaaaaa agcgcgattt ttcttcatgc tgactaacta attacacctt ctccggctgt
    10921 ggagaaagct ccgacagcat ccagcgcttc aggcaactcc cgcgatgata aaccattgcg
    10981 ggagataacc ttgattactt cagatcggcc tgtacctgac gcagcggttt cgcccacggg
    11041 tttttggcct ggacatctgc tggcaatgta gataccgctt ttttcgcctc ttctttcgaa
    11101 gcgtacacgc cagaaaccag gacataccac ggctgaccat tacgcgtcgt ttcatagaca
    11161 acgtagtttt tcagattctc tttcttcgcc caaccgttca ggttgtcgta gttagaggaa
    11221 ctgctcagct gcagagtgta atggctggac ggtgccgatt tcaacgaacc aacattacct
    11281 gcggtcttcc ctccagcggc tggtgttgcc gtggtttgcg ccggggatgc cgtctgtact
    11341 ggagccgtag ttgcggtctc ttttggtgct ggcgtagagg tcgcagccgg tgccttcgtg
    11401 cttgccacag gagcagctgg ttcagtacgc ttcggcgtct gtgctaccgg cttcggctcc
    11461 gttttcacgg ttgcttgcgg ttttttcggt tcaatcaccg cctgctggcg agctggacgc
    11521 gtagtggccg gatgttcagc ggtttgcgtt ttcgccgtgt cacgcgatgc attgccattg
    11581 cgaacaggcg cgaccgtcgc gggttcagtc ggcaacgtgg aattgaccgc cacattattc
    11641 aactgttgct gattttgtgg ctgggtcagc gcattgttca ggtcaccctg cacttcaaca
    11701 cgttgttgac catccgttgc cgccggggtt tgcccttgag tcggcgtaga agagatcggc
    11761 ggcagagaaa catcctgctg agtattttcc gcagacgtgg ttcccggcgc tggctgcacg
    11821 ccattcgcct gatcggtcgc attgccagca agatcaatac tcttctcgcc agacgcggtt
    11881 tgatcgctgg aagaggtcga gggggctttt agcgcagaac cgataccaat gatcaacagc
    11941 aggagaacca gaatgccgac gcccatcatc atatactgac gagaagcggg tttactggct
    12001 gcttttttgc gcttacgcgg acgacgctct acgcgctctt catccacggt ttcatcttcg
    12061 gattcgtcaa tttcttcttc gagttccggt tcctcattgc gctctttttg cgcacgagtc
    12121 ggacggcgat cgtcagtgtc atcaagttca atatcatcaa aattgatctg cggttcgcca
    12181 cgttcagtac gctcagaacg ttcagaagat tgacgagaac gaccagtacg acgatcgctg
    12241 ggatcgggtt tcagctcgtc ttctggtttg aattcatcca tttaacaccc cactaaaagg
    12301 ttaatgctta ccacgttgca attaacctga agctaataga ccgcttgata agcggcctga
    12361 cctttcttgt tgttacgctg attgacaatc ggcaatggcg ttaagaacaa gctcgtgcga
    12421 aacgccgctg cgaacttcac tcttaccaat tgccaacgga agaattaagc gcatctctcc
    12481 cgcaaggact ttcttgtcac gcagcatatg cggtaaatac gcctgcgcag acatttcgcg
    12541 cggcccattg accggtaacc cagcccgcgt gagcagggtt ataatacgct gcgtttcggc
    12601 agaactaaac tgccctagac gttccgacgt ccgcgccgcc atcaccatac ccgcagcaac
    12661 cgcttcaccg tgtaaccaat tgccataccc catttcagct tcaatggcat gaccaaaggt
    12721 gtgtcccaga ttcagtaaag cacgtaaccc ggtttcgcgc tcgtcggcgg cgacaacttc
    12781 tgccttcagt tcacaacaac ggcgaataca gtacgccatt gccggaccgt ccagacgcaa
    12841 caacgcatcc agattttctt ccagccagtt aaaaaacgca ccgtcaagaa taatgccgta
    12901 tttgatgact tctgccagcc ccgacgctaa ctcacgcggg ggaagcgttt tcagacagtc
    12961 gagatccacc accactgaag caggctggta gaacgcgcca atcatgtttt taccgagggg
    13021 atggttgacc gcagttttgc cgccaacgga ggaatcgacc tgcgacagca acgtcgtcgg
    13081 gacttgaatg aaacgtacac cgcgctgata actcgccgcc gcaaagccgg tcagatcgcc
    13141 cactacgccg ccgccaagcg ccaccagcgt agtatcgcga ccatgcggct tttgtaacaa
    13201 cgccgtaaag acggtatcga gtacagccag gcttttatac tgctcgccgt cagggaggat
    13261 aacgctatcg acgttaacac ccgcctgttc aagtacgccg cggaccttat cgagatacag
    13321 aggaaccagg gtttcgttgg taaccaacat gacctgctcg cccgatttca gcggtaagaa
    13381 tgaagctggt tcattaaaca aaccagatgc gatggtaatt gggtaactac gttccccgag
    13441 agtaacgaca atcctctcca taacgcgaca tccaccttaa ttactgtacc cgcagacgag
    13501 tgtatataaa gccagaatta gttgctttcc agcatgtgaa taatctggtt tgcgaccact
    13561 ttagcgcttt gatcatcagt acgaatggtc acgtcggcaa tctcttcata cagcggattg
    13621 cgttcattgg ccaacgcttc cagaacttca cgcggcggtg tttcaacgtg cagcaacggg
    13681 cgttttttat cacgctgcgt gcgtgcaagt tgcttttcga tggtcgtttc aagataaacg
    13741 acaacgccac gtgcggaaag acggttacgc gtttcgcggg atttcacaga gccgccgcca
    13801 gtagccagca caataccctg tttctcggtc aactcattga tgaccttttc ttcgcgatcg
    13861 cggaagcctt cttcgccttc taaatcgaaa acccagccca catcagctcc ggttcgtttc
    13921 tcaatctctt gatcggaatc gtaaaattcc atattgagtt gttgagctaa ctggcgccca
    13981 atagtgcttt ttccggcacc cataggccca accagaaaga tattgcgttt ctctgccatt
    14041 ttttcggtac tactaagact attcgttaat gataaacccg cttcgctcag agagcgccgc
    14101 aggacatgaa ctgaaacctc ataagatatt gcgagagtca gactgaaaat tatctcaata
    14161 ctccagcggg tttggcaact gaataaatca cccatccctc tgcatatctg gtcgctgcaa
    14221 gcgcgctgcc ttgctaccac cgctctggcg ataaatcacc gggtaagatt agcgtaaaaa
    14281 agacagcaaa atgccgcctg aatgataaat catcatcatg gaacgcaggc agcgaagagc
    14341 accaactctc aaatcggtac tccttgtatg ctaaatacct gcgcgcgtca aatagatgaa
    14401 acacgttcag cgtaaaaaca ttaccgcttt tacggctgtt tactcactgg aaaccagtcg
    14461 tggcgtgata aacaccacta actcgcgtcg ttcatcttct tttccgtcat gacgaaataa
    14521 ttgcccgaac cagggaatgt cgccaagcaa cggtacgcta tcctgacccg atttattttt
    14581 acgggtaaaa atgccgccca gcgccaacgt ttctccgctt ttgacctcga cctgcgtttc
    14641 gatctcctgc ttatcaatcg ccaacacttc gccatcggcc tgctgtagca cctgccccgg
    14701 aacgttctgg ctgatgtgta atttcagccg gatgcgacct ttttgtaaca ccgtgggcgt
    14761 aacttccatc cccaggacgg cctctttaaa ttccaccgac gtcgcgccac tttccccgct
    14821 ggaaacctga tatggaattt cgctcccctg tttaatgctg gcaggctgaa gatgtgaggc
    14881 aagcagacgc ggactggcga taatatccag ctgctgtttt tgttcgagcg cggaaagctc
    14941 aagatccagt aaacgtccgt tgatgcgtcc aatgttaaaa ccgacatgcg ttgtcgccgt
    15001 cgctacggag aggtcgctgc caagcgtggt gacttgccca acgccaccag cttgttgcgc
    15061 atcggccagc gtccatttca cgcctaactc acgcaaactt ttttcattaa tggtgacaat
    15121 atgcgccgac agctcaacct gcccgaccgg cagatccatt tgcgctaccc actgttcaag
    15181 ggcgcttaac gccgttttgt tatctcgcag caaaaggcga ttggtgcgtt tatcgacggt
    15241 catactccct ttggcactca gtagcttctc cccagctttc gccagctctc cggcgtcggc
    15301 gtattgcaga gtaatattgc gattttccag cggcagattt gcctgcgccc gcgcctgctc
    15361 cgcttcctga cgggcgatat tgtcattctg ccaggcaatg gaatgcactg agagaatgtt
    15421 gccctcctga cgcgttatca gtccggcgct tttcactaca gtttgtagtg cttgcttcca
    15481 gggaacatct gttagatgta acgacaccgt accgctgacg tctggcgaca ccaccaggtt
    15541 caacttctcc tgttcagcca gcgcctgcaa cacctgagct accggaacgt catccaccat
    15601 cagcgtcact ttttgcggct ttgccgcctg tacgccgggt atcagcatca acagtagtgc
    15661 ggctatccat tgcttcattt gtatctcctt gccgttgcca caaccagtgt ggcggttcgc
    15721 agtttgtccc ggttcccagc gttagtgcgt ctggcgttag ctgtaagatc gtccagccgt
    15781 tttccagcac atcgttttgc tgcacccgtc gccatttttt ttgcccgtct tttattacac
    15841 cgatgatgcg ctcgcctcgc cctaccatcc cctgatagcg ccactggcta agttcgctaa
    15901 tccggcatag atcttccggc ggtttaaaag ggtcacgcat accggttaaa aggcacaatg
    15961 caatacctgc caacaaccag cgtttacccc tcatgcggcg tctccagttg tagcgtgaac
    16021 aaaaggtcat caccttccac gcttaacgaa aaacggctca cgctgacgtt gcgctctgcc
    16081 agccgtgtaa atgccaacgg cactgcttcc cacagcgttt tcaacgccaa ctcacctccc
    16141 tgcgcggatg gacgccagga aaccagttgc gcgccggata actgaaaatc cagtggcgaa
    16201 aagggcagcg ttttttcttc gctgaaggga gtggtgtcta ccaggcgata cagtgcggcc
    16261 cactgctggt gatgactcgc ccgtagtcga attaatgcgt cgctctcttc ctggtgtgtc
    16321 gatgacagaa aaatgagcgt aactaacatc agcaaccaga atgcccagca aagctggcgg
    16381 aggcggggtg atgtggcgaa ccaccagtca aagagcatgt ccatcgctaa ccttccttgt
    16441 taactgatac tcaaattgcc agcgtccctg cgcatcctgc tgcgtggccc cccgctgatt
    16501 gagatgaaaa gatgcatcct ggcggagtga cgtttccagc gcgtttaacg cggtaatgct
    16561 tgttgtcagc cccttgatct ccagcgttcc ctgctgccag cttatcgttg tcagccaggc
    16621 atgttcgggt aaaagcgccg ccagtgcttc cagcgcagat tgccagtcac gggtaaattg
    16681 tcgctggagc tgcctttgca aacgctgttc gcgtaactgc tggcgttcca gcaaacgtgg
    16741 tttcgttatc tgtaagctgc gggcgagttg ttgttccgcc tgaagcaaaa cggtgtctac
    16801 gcgtgcttcg gcgttgcttg tcagacgcag tattagcgtt atcccgacag ccagcagcag
    16861 aggcgcaacg aacatcagca accagaaacg aagaaaagcg gtccggcgtt gctgtcgcca
    16921 gggcaaaaaa ttaattggcg ggttcatcag tacaccttcc caagcgccag ccccagcgcg
    16981 atggcaaagt ctccacctgg cggcggtagc ggcggctgac gaacagaaac cgcctcccag
    17041 gggtcaaatc cgccttcgcc acatatcgcg acgctttcgg gatcaacgga taacgctgcc
    17101 gccagctcct tcgcgctagt tatccccacc gccagtttgc gcccccagct atagcgcgtc
    17161 gcccacagcc actgttcgtt atcacgccag gccagacatt gctgatgaga aggtaaaaaa
    17221 ggcaggaatc gctgtaatgc actggcatcc ggggtgatcg cgctaacatg aacacgcaac
    17281 ctttctgcca gagtaagcag cgttgccaac tctttgcttt gcgcggcagt cacgttatag
    17341 gcgggactga gtgagtcttc gctgtaatcg aagcgcaggg agtccggatc catatccagc
    17401 tcgcgggcca tcgtcccgga cagccaggcc gtttgctccc gctcaccaag ggacatcgac
    17461 gggcgtggaa atgaccgctg taatgtgcga ctggcgggaa acgccaacat aatgtgatga
    17521 cgctgcggca gttcgcgact ccacggtaac aacgtgttag ccagtcgctg cgcatcaaca
    17581 atccgcccat ctttgattat gtcgttctcc agcggcaacc gccaccagcg ttgcaaaaaa
    17641 cattcttttg tgccccgcac gatagcaacc gctaccgctt cttgctgttg taaatgcaaa
    17701 ccaatttgcc agatcttaaa tgccattgtg atgatctcct tatcacccgt cactctgacg
    17761 ggtatatcaa tgcgtctggc ttgcctttat actaccgcgc gtttgtttat aaactgccca
    17821 aatgaaacta aatgggaaat ttccagtgaa gttcgtaaag tattttttga tccttgcagt
    17881 ctgttgcatt ctgctgggag caggctcgat ttatggccta taccgctaca tcgagccaca
    17941 actgccggat gtggcgacat taaaagatgt tcgcctgcaa attccgatgc agatttacag
    18001 cgccgatggc gagctgatcg ctcaatacgg tgagaaacgt cgtattccgg ttacgttgga
    18061 tcaaatcccg ccggagatgg tgaaagcctt tatcgcgacg gaagacagcc gcttctacga
    18121 gcatcacggc gttgacccgg tggggatctt ccgtgcggca agtgtggcgc tgttctccgg
    18181 ccacgcgtca caaggggcga gtaccattac ccagcagctg gcgagaaact tcttcctcag
    18241 tccagaacgc acgctgatgc gtaagattaa ggaagtcttc ctcgcgattc gcattgaaca
    18301 attgctgacg aaagacgaga tcctcgagct ttatctgaac aagatttacc tcggttaccg
    18361 cgcctatggt gtcggtgctg cggcacaagt ctatttcggt aaaacggtcg accaactgac
    18421 gctgaacgaa atggcggtga tagccgggct gccgaaagcg ccttccacct ttaatccgct
    18481 ctactcaatg gatcgtgccg tcgcgcggcg taacgtcgta ctgtcgcgga tgctggatga
    18541 agggtatatc acccagcaac agttcgatca gacacgcact gaggcgatta acgctaacta
    18601 tcacgcgccg gagattgctt tctctgcgcc gtacctgagc gaaatggtgc gccaggagat
    18661 gtataaccgt tatggcgaaa gtgcctatga agacggttat cgcatttaca ccaccatcac
    18721 ccgcaaagtg cagcaggccg cacagcaggc ggtacgtaat aacgtgctgg actacgacat
    18781 gcgccacggc tatcgcggcc cggcaaatgt gctgtggaaa gtgggcgagt cggcgtggga
    18841 taacaacaag attaccgata cgttgaaggc gctgccgacc tatggtcctc tgctacctgc
    18901 cgcagtcacc agcgccaatc ctcaggaagc gacggcgatg ctggcggacg ggtcgaccgt
    18961 cgcattgagt atggaaggcg ttcgctgggc gcgtccttac cgttcggata ctcagcaggg
    19021 accgacgccg cgtaaagtga ccgatgttct gcaaacgggt cagcaaatct gggttcgtca
    19081 ggttggcgat gcttggtggc tggcacaagt gccggaagtg aactcggcgc tggtgtcgat
    19141 caatccgcaa aacggtgccg ttatggcgct ggtcggtggc tttgatttca atcagagcaa
    19201 gtttaaccgc gccacccagg cactgcgtca ggtgggttcc aacatcaaac cgttcctcta
    19261 caccgcggcg atggataaag gtctgacgct ggcaagtatg ttgaacgatg tgccaatttc
    19321 tcgctgggac gcaggtgccg gttctgactg gcagccgaaa aactcgccgc cacaatatgc
    19381 tggtccaatt cgcttacgtc agggactggg ccagtcgaaa aacgtggtga tggtacgcgc
    19441 aatgcgggcg atgggcgtcg actacgctgc agaatatctg caacgcttcg gcttcccggc
    19501 gcaaaacatt gtccacaccg aatcgctggc gctgggttca gcgtcattca ccccaatgca
    19561 ggtggcgcgc ggctacgcgg tcatggcgaa cggcggcttc ctggtggacc cgtggtttat
    19621 cagcaaaatt gaaaacgatc agggcggcgt gattttcgaa gcgaaaccga aagtagcctg
    19681 cccggaatgc gatattccgg taatttacgg tgatacgcag aaatcgaacg tgctggaaaa
    19741 taacgatgtt gaagatgtcg ctatctcccg cgagcagcag aatgtttctg tgccaatgcc
    19801 gcagctggag caggcaaatc aggcgttagt ggcgaagact ggcgcgcagg agtacgcacc
    19861 gcacgtcatc aacactccgc tggcattcct gattaagagt gctttgaaca ccaatatctt
    19921 tggtgagcca ggctggcagg gtaccggctg gcgtgcaggt cgtgatttgc agcgtcgcga
    19981 tatcggcggg aaaaccggta ccactaacag ttcgaaagat gcgtggtttt cgggttacgg
    20041 tccgggcgtt gtgacctcgg tctggattgg ctttgatgat caccgtcgta atctcggtca
    20101 tacaacggct tccggagcga ttaaagatca gatctcaggt tacgaaggcg gtgccaagag
    20161 tgcccagcct gcatgggacg cttatatgaa agccgttctt gaaggtgtgc cggagcagcc
    20221 gctgacgccg ccgccgggta ttgtgacggt gaatatcgat cgcagcaccg ggcagttagc
    20281 taatggtggc aacagccgcg aagagtattt catcgaaggt acgcagccga cacaacaggc
    20341 ggtgcacgag gtgggaacga ccattatcga taatggcgag gcacaggaat tgttctgatt
    20401 aaaaaggcgc ttcagcgcct ttttaagttt gctgacaaag tgcactttgt ttataccgga
    20461 tacggcgtga acactttacc cgtctagcaa catctagaaa aatcaatata ttgcaggagc
    20521 cgcacaggcc tgataagcat tatgcatcag gcaagcttca cgcatttaca accgcccctg
    20581 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    20641 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    20701 tggctcatcg ccttccagcg attccgggta gagatcttgc gctaccacga tattcatttt
    20761 gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    20821 aaatcccacc tcttctttta gctcgcggtt cgcggcttcg aagacgcttt cacccggatc
    20881 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    20941 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    21001 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc tttcgacggt
    21061 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    21121 gctcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    21181 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    21241 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    21301 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaaactcca
    21361 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    21421 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    21481 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc
    21541 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacatagcgc
    21601 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacgggagc
    21661 cagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac
    21721 acacgctatc acgcgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    21781 cgattcggta gaagtccacc tgcccccttt ctgggagcag tacatcaacg atgagaatac
    21841 cgttgaactg attcatacca attcgctgcc gctggttatt tcactcaacg gtcatacgct
    21901 gcaggagtac atgcaggaaa ctcgcggcta tgccttgcaa cctgttccgt caacacaggc
    21961 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    22021 cgaagagtat gcgctaagtc gtccgcgcgg gctgcgtgaa gcgttgctga ttgtcgcctc
    22081 attcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    22141 cggcgcgtta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    22201 ctccctgcgg gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    22261 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctaccccgc
    22321 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatatcg atatctatct
    22381 cgaccgccac gtagtgcgtc aggggcgata tctttcgctc catgatgaag taaaaaactt
    22441 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctac tggtgctgtt
    22501 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcaccctct cgtggatgaa
    22561 aggcgcgcag accattgaag ccaccagcgt gaaacaactg gctgacgctg gcgtgcgggt
    22621 tggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggtacctg
    22681 gagcgcgaaa accaactcac cttttttacc gtttgactgc tcgcagatca tctggaatga
    22741 cgcccgctca ttgccattac cagaatctga attggtcaac aaagcgacgg cattgaccga
    22801 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gcgcctcatt
    22861 acgttcagca attcaaaaat ccggcatggt attgcttgat gattttggcg acatagtact
    22921 gaagacagcg gatctgtgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    22981 caatctcggt aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaaact
    23041 cgatggcgtg aatgtgttat tacgcccggt gagtgcggaa tcgctggata acctggtggc
    23101 cacctctacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    23161 tccggccccc ggcgggttcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    23221 ctggccttcg gcatcacttt acgactatcc gccgcaagaa cagtggaacg ctttccagaa
    23281 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    23341 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    23401 gtggcgctat ctcagcacca cgctgctgct actgacgatg ctgggtagcg ccatttataa
    23461 tggcgtacag gcctggcgtc gttaccagcg tcatcgtact cgcatgatga agattcaggc
    23521 ctattatgaa agctgcctga acccacaact gatcacccct tcagaaagcc ttatcgaata
    23581 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    23641 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    23701 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    23761 ggggcgcgaa aaacggggtt acgccacagg aagcgaggga atatatgcgc cagcaatatc
    23821 acgacgtaca gcatacgcta aactggtact gttttgatta ctggagtgag caactgggtc
    23881 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    23941 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    24001 atccgcacaa cctggcggta aaacttgagc ataccggtct ggacgcacac cttgatttat
    24061 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    24121 ccgaagctac gggactgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    24181 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgactcag
    24241 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    24301 cctcgctaat gtgaaggaga tgccatgaaa gaaaaacctg ctgttgaggt tcgactggat
    24361 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa
    24421 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat
    24481 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    24541 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    24601 gagaaacgcg aaaaaatggc gctggcacgt aaacttaatg ccttaaccat gccgcacccg
    24661 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    24721 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    24781 ctgtttgaaa actttgccgt gcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    24841 atccttgaga atcacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    24901 gcgaccagcc tgttaaccgc tacgctgaaa tttgatggtg atatcaccgt acagctgcaa
    24961 ggcgacggcc caatgaatct ggcagtcatc aacggaaaca acaaacagca gatgcgtggc
    25021 gtcgcgcgcg tacagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    25081 ggttacgtgg tgatcaccat caccccgagc gaaggtgaac gctatcaggg cgtggtgggt
    25141 ctggaaggtg ataccctggc ggcctgcctg gaagattact ttatgcgttc tgaacagctg
    25201 ccgacgcgcc tttttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    25261 ttgttgcagg taatgcctgc gcaaaatgcc cagcaggacg actttgacca cctggcgacg
    25321 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    25381 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    25441 acctgctcgc gtgaacgttg cgccgacgcg ctaaaaacgc tgccggatga agaagtcgat
    25501 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    25561 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgcaa
    25621 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac taatacacct
    25681 gatgcgcttt gtcactcatt cagattcaca tccagccttt caccatccac tacaagatcg
    25741 cgccacgctt taggcttcgt tcccagtttt ccatccttcg cagctgtgag taatttttct
    25801 ttcgttatat cacgcggaag taaactcatc gtaagctctt gccattcttt cttgtcagga
    25861 tcaaagccat agacaatgta tctttcatca tcaaaggcaa agaggatccg ctctgccctg
    25921 ccatcactat tcagatcttg ctcgaccagt acgcaggcgt cttttccaat acaggtcatc
    25981 acgttatagc gatcctggat taaggccgac cagaatgtcg catccggttt accagaacca
    26041 ggggcaatta acacattttc ggctaatact ttttccgata tctgttgctg aagatgttgc
    26101 tctccatcta acgccatcaa cagatcccgc gcgcgtttcg ggtctttcat aaacccggca
    26161 tcgcttttca gcgactcaag cgccgcacgt ccatagcgac cgctctgttc gagcatatag
    26221 agacttacct ggtctggcgt gtttttgccg ctctgataac gcgccatatg actgttcacg
    26281 ctaatgcgca tactgtccag caccggagaa ttaagaagca ccagtattac caacaccaat
    26341 aacgaaaccg caaggttcac tttgccctga agaacaaggg ggttttgccc tttacgccag
    26401 acgatactga caaaataccc cagcgaccat accagtaaca ccagcaccgc cagcgcaccc
    26461 tgcaagcggt cgacagtcca gccgtactga gcgacccgca gccataacgc ccaggcggcg
    26521 acgaacacat acagcggagc aaccagcaga gcggttttaa tcaggcaacg caacggcccc
    26581 gtccagggaa gtgacgattt ttgcggatcg cggacaatag ccattaagat caattgcaaa
    26641 aaggccagcg tcaacagcag cccggcggcg gagatgtggc gagaaatcgc gctcaggccc
    26701 gtaaacggca gggtgatgat aaacatcagg gttagcaatg atactaacgg cagcaacccc
    26761 gtggcgatta acgttaacaa cttttgaata gagtcgatta aacgtgactg tgttcgcgcg
    26821 aggatcaccg ccagcgcggt aaccagacct aacgtgagat aaataaacca gtcggttgca
    26881 aaaaagagcg ttttgaaaaa cgtgatgcca acaagtttaa acaactcgcc ccacagtaaa
    26941 agcaccagcc acgtcaggcc attggcgaga aaaatcacca gtaatataag tacgttatgc
    27001 catactgact ggtaaaaata gcgatagcgg ttactgtcat tgcggatgcg aaggctttgt
    27061 tgtatccacg gtagcaacaa cattgccatc agcagcagat agcagccaaa atcccaaagc
    27121 gccttttctg ctctccaggg gttcatgcca tcagtttgcc acttcagcca gccgctcatt
    27181 cccagcgtgg caataaacac cagcgccagc catccccaaa ggcgcttctg tttaaaggag
    27241 atcacggaga agagtaaaac ggatgaaaac gcgacggtcg caggcacgcc ataaacaatc
    27301 caactgagat tttttccggc aagccaggcg atcagtaggt agcaaaccag accctgaagt
    27361 aatccggtag caatcatccc ccagcgtgtt gcgggtgaaa gttcaacgtt atccatgtta
    27421 gttatccaga atcaaaacgt gggttaatta tcgcatccgg gccgtagtat tttgcttttt
    27481 tcagaaaata atcaaaaaaa gttagcgtgg tgaatcgata ctttaccgat tgaatttgca
    27541 tcaatttcat tcaggaatgc gattccactc acaatattcc cgccatataa accaagattt
    27601 aaccttttaa gaacattttc cacacctaaa atgctatttc tgcgataata gcaaccgttt
    27661 cgtgacagga atcacggagt tttttgtcaa atatgaattt ctccagatac gtaaatctat
    27721 gagccttgtc gcggttaaca cccccaaaaa gactttacta ttcaggcaat acatattggc
    27781 taaggagcag tgaaatgcgc gttaacaatg gtttgacccc gcaagaactc gaggcttatg
    27841 gtatcagtga cgtacatgat atcgtttaca acccaagcta cgacctgctg tatcaggaag
    27901 agctcgatcc gagcctgaca ggttatgagc gcggggtgtt aactaatctg ggtgccgttg
    27961 ccgtcgatac cgggatcttc accggtcgtt caccaaaaga taagtatatc gtccgtgacg
    28021 ataccactcg cgatactttc tggtgggcag acaaaggcaa aggtaagaac gacaacaaac
    28081 ctctctctcc ggaaacctgg cagcatctga aaggcctggt gaccaaacag ctttccggca
    28141 aacgtctgtt cgttgtcgac gccttctgtg gtgcgaaccc ggatactcgt ctttccgtcc
    28201 gtttcatcac cgaagtggcc tggcaggcgc attttgtcaa aaacatgttt attcgcccga
    28261 gcgatgaaga gctggcaggt ttcaaaccag actttatcgt tatgaacggc gcgaagtgca
    28321 ctaacccgca gtggaaagaa cagggtctga actccgaaaa cttcgtggcg tttaacctga
    28381 ccgagcgcat gcagctgatt ggcggcacct ggtacggcgg cgaaatgaag aaagggatgt
    28441 tctcaatgat gaactacctg ctgccgctga aaggtatcgc ttctatgcac tgctccgcca
    28501 acgttggcga gaaaggcgat gttgcggtgt tcttcggcct ttccggcacc ggtaaaacca
    28561 ccctttccac cgacccgaaa cgtcgcctga ttggcgatga cgaacacggc tgggacgatg
    28621 acggtgtgtt taactttgaa ggcggctgct acgcaaaaac tatcaagctg tcgaaagaag
    28681 cggaacctga aatctacaac gctatccgtc gtgatgcgct gctggaaaac gtcaccgtgc
    28741 gtgaagatgg cactatcgac tttgatgatg gttcaaaaac cgagaacacc cgcgtttctt
    28801 atccgatcta tcacatcgaa aacatcgtta agccggtgtc caaagcgggc cacgcaacta
    28861 aagttatctt cctgaccgca gacgcctttg gtgtgctgcc gccggtttct cgtctgactg
    28921 ccgatcaaac ccagtaccac ttcctctctg gcttcaccgc caaactggct ggtacagagc
    28981 gcggcatcac cgaaccgacg ccaaccttct ccgcctgctt cggcgcggca ttcctgtcgc
    29041 tgcacccgac gcagtacgca gaagtgctgg tgaaacgtat gcaggcggca ggcgcgcagg
    29101 cgtatctggt taacaccggc tggaacggca ctggcaaacg tatctccatt aaagataccc
    29161 gcgccattat cgacgccatc ctcaacggtt cgctggataa cgcagaaacc ttcactctgc
    29221 cgatgtttaa cctggcgatc ccaaccgaac tgccgggcgt agacaccagg atcctcgatc
    29281 cgcgtaacac ctacgcctct ccggaacagt ggcaggaaaa agctgaaacg ctggcgaaac
    29341 tgtttatcga caacttcgat aaatacaccg acacccctgc gggtgccgcg ctggtagcgg
    29401 ctggtccgaa gctgtaatca gctgaaatct cattgacccc ttatccaaac ggataagggg
    29461 tttttttata tctaattagc taccaatatg tacacggtac acttataatt atcaataaac
    29521 cgcaggatgc attatgttca cgtttattga actacaagga tttagcaaac gccgccagtt
    29581 attgttacct gatgatgagt tccgggcttt tcaggaacta ctcattcaag atcccactgc
    29641 cggtgacatt atcgcaggca caggcagttt tagaaaaatt cgctggaacc gttctggaat
    29701 ggggaaacgg ggtggcattc ggataattta ttacaacgta acacgcaatg gccgaattta
    29761 tctggcgttg atttatccta aaaatgaaca ggatgattta acagaagaac aaagaaaggc
    29821 attgaaacta ttgtccgaga agctgcttta atgttgaagc tccacaaatt ccaccatggc
    29881 cgaaatgcca acggaggcat tgaaaacaga tgaaagacga attatttgct gatcttcttg
    29941 ccagcgcaga agaaatggta cgcatcgaaa agggtgaaga agcgcctgaa ccagagcacg
    30001 tccatacttt tagcgaaatc gatgtaaaag cgatccgtga agcaacgggt ttacgtcagc
    30061 aagattttgc tgtcgccgta ggggtaagtt atgacctggt caaaagctgg gaaaccaaac
    30121 gccgtcaacc taccggagca ccccgaaaat tgctcctgct tttgcaagcg aatcctttca
    30181 ttatcaatca attgaaaaca atctaaaaac aggaggcacc ttcgcctccc gttttattta
    30241 cccttctttt gtcgcaccct gcgcccgcgt taccggcaca ggcagccagg cgcgaatgga
    30301 aagcccgccc cgttcgctgg tgccaagctc cagcatcccg ttatggttat ccacgatacg
    30361 ctgcacaatc gccagcccta atcccgtgcc gctaatggtg cgcgcactgt cgccgcgaac
    30421 aaacggctgg aacaggtgct tacgttgttc cggcgcaatt cccggaccgt catcttccac
    30481 ctggaaccag gcgcgattcg gctccgttcc gctgctgact ttgatccagc cattgccgta
    30541 acgggcggcg ttgaccacca tattcgccac cgcacgtttg atcgacagcg ggtgcatttt
    30601 cacctcaatg ctgccggggt aaagcgcggt ttcaatttcc cgctcatagc cactttcggc
    30661 ggcaatcacc tcaccgagca ctgcgttgag atccgccatt tccatcggca tctcctgccc
    30721 ggtgcgcagg tagtcgataa actgctcaat gatggcgttg cactcttcga tatctttatt
    30781 gatcgattct gccaggtagc catcctgctc gctcatcatc tcggtagcca ggcgaatgcg
    30841 cgtcagcggc gtacgcagat catgacttac ccccgccatc agcagcgtgc gatcatccgc
    30901 cagttgctta acaccagccg ccatatggtt aaaggcgcgg gtaacggaac gcacctcgga
    30961 cgcgccatat tcacgcagcg gcggcggaat aatcccttta cccacctgca aggctgcgtg
    31021 ttcgagatcg accaacggtc ggttctggat acggataaac agccacgccc cgcctatcgc
    31081 caatagcata atcgccagcg tatagcggaa cagcggggag aaatcgccct gatgaatttc
    31141 ggtcagcggc acgcgcaccc agatattggg cgacagccag gttttcagcc agacgacagg
    31201 agaacttttg ttgacctcaa cgcgcacttc cgtcgggccg cccagttgct gcgccatctg
    31261 atggcttaag aattcatagt gttgtgccca acgcagaccg gcctcttcgg cagcttcgtt
    31321 ggaatagaga gagatcccca gctcacggta gatctcccga cggaaagcgg gaggcacaac
    31381 caactgcgtg ccgtcctcca gttgtagttt gtcggtcatc aacatacgca cttcgtatgc
    31441 gaggacttta ttaaactgct ggaggctcgg caaaatcgcg aagttcagta ccaccagata
    31501 agtcgtcacc aggctgacga acagcaaggt gacgatgagc aataacgtac gggcaaatga
    31561 acttcgtggc gagaagcgca atcgcctcat gctttagaac cgtccggtac aaagacgtag
    31621 cccagacccc agacggtctg aatgtaacgc ggatgcgccg gatcttcttc caccatgcgg
    31681 cgcagacgcg aaatctgcac gtcgatggaa cgttccattg cggagtattc acggccacgg
    31741 gcaaggttca tcagcttatc gcgggagagc ggctcacgcg gatggctgac caatgccttc
    31801 agtaccgcaa actcaccgct ggtgagcggc atcggctcat cttcgcggaa catttcacgc
    31861 gtaccgaggt taagtttgaa cttaccgaaa gcaattaccg cctcttcctg tgacggcgcg
    31921 cccggcagtt cgttggcctg acgacgcagc accgcacgaa tgcgggccag cagttcacgc
    31981 gggttaaacg gttttggaat gtagtcgtca gcgccaatct ccaggccaac gatacggtcc
    32041 acttcttccc ctttcgccgt caccataatg atcggcatcg ggttgctctg gctacgaaga
    32101 cgtcggcaaa tcgacaagcc atcttcacca ggtaacatca aatccagtac cataagatgg
    32161 aaagattcac gagtcagcag gcgatccatc tgttctgcat tagcgacgct tcgaacctgg
    32221 aagccttgtt cggtgagata acgttccagc agcgcgcgca ggcgcatatc gtcatcgacc
    32281 accagaatct tgtagttctc ttgcattgtt tgtactccca aaggttcgca acaatttgta
    32341 agcgtgtatt cttaaaaaag ctcacgttcg tcaccagcta aatctggtat gaatttcagc
    32401 ctaaattgtt acaaagcata ttaaacagca gcttaagtat acaatttatt cggcgaaaca
    32461 ttattgattc tgttgatatg atcacgttat acccaatgtg cgcattatca aacagacaaa
    32521 gggaatcaac gagatgaaaa cgcccctggt tacccgggaa gggtatgaaa aactcaaaca
    32581 agagcttaat tatctctggc gtgaagaacg cccggaggtc acaaaaaagg tgacctgggc
    32641 cgcaagtctg ggcgaccgca gcgaaaatgc tgactatcag tataataaaa agcgcctgcg
    32701 tgaaatcgac cgtcgcgtgc gctatctcac taaatgcatg gaaaatctca aaatcgtcga
    32761 ttactccccg cagcaggaag gcaaagtctt ttttggcgcg tgggtggaga ttgaaaacga
    32821 cgatggcgtg actcaccgtt tccgtattgt cggctacgat gaaatttttg gccgtaaaga
    32881 ttacatctct atcgattccc cgatggctcg cgcattgctg aaaaaagagg tgggcgacct
    32941 ggcggtggtc aatacccctg ccggggaagc gagctggtat gttaatgcta tcgagtacgt
    33001 gaaaccgtaa ggaagagtct taacctcctg ccgatggctg gcatttttgc cagccagtcc
    33061 gtataactat cccctgattt ttgatccgaa aagatgaact caaaccatga tgaatgattc
    33121 gttctgccgc attattgcgg gtgaaattca ggcgcgcccg gaacaggttg acgctgccgt
    33181 tcgcctgctt gacgaaggga ataccgtgcc gtttatcgca cgttatcgta aggaaatcac
    33241 cggcggtctg gatgacacgc agctgcgtaa tctggaaacg cgtctgagct atctgcgcga
    33301 gctggaagag agacgtcagg cgatcctcaa gtccatttcc gagcaaggca aactcactga
    33361 cgagctggcg aatgccatca acgccacact aagcaaaacc gaactcgaag acctctacct
    33421 gccctacaaa cctaaacgcc gcacccgcgg gcaaatcgcc attgaagcag ggcttgagcc
    33481 gttggccgat ctgctgtgga gcgatccgtc acacacgcca gaagtcgccg ctgcacaata
    33541 tgttgatgcc gataaaggcg tggcagatac caaagccgcg ctggacggcg cgcgctatat
    33601 cctgatggaa cggtttgccg aagatgccgc gctgctggcg aaagtgcgtg attatctgtg
    33661 gaagaacgcg catttggttt ctacggtggt gagcggtaaa gaagaggaag gggcgaaatt
    33721 ccgcgactat ttcgatcatc acgaaccgct gtccacggtg ccttctcacc gcgcgttggc
    33781 gatgttccgt gggcgtaacg aaggcgtact tcagctttcg ctgaatgccg atccacagtt
    33841 cgatgagccg ccgaaagaga gctattgcga gcaaatcatc atggatcacc ttggcctgcg
    33901 cctgaacaat gccccggcgg atagctggcg caaaggcgtg gtgagctgga cgtggcgcat
    33961 caaggtgctg atgcatctgg aaaccgaact gatgggcacc gtgcgcgaac gcgcggaaga
    34021 tgaagcaatc aacgtctttg cccgtaacct gcacgatctg ctgatggcgg ccccagccgg
    34081 actgcgtgca acgatgggcc tcgatccggg cctgcgtacc ggggtaaaag tagcggtggt
    34141 cgatgccact ggcaaactgg tggcgaccga taccatttac ccgcacaccg gacaagccgc
    34201 aaaagcagcg atgaccgttg ccgccctgtg tgaaaagcat aatgttgaac tggtggcgat
    34261 cggcaacggt acagcttccc gcgaaactga gcgtttctat ctcgacgtgc agaagcagtt
    34321 cccgaaagtg accgcgcaga aagtgatcgt cagcgaagct ggcgcgtcgg tttactcggc
    34381 ttccgagctg gcagcgcagg agttcccgga tctcgacgtt tcgctgcgtg gcgcggtgtc
    34441 tatcgcccgt cgtttgcagg atccgctagc ggagctggtg aaaatcgatc cgaaatctat
    34501 cggcgtaggt cagtatcagc atgacgtcag ccagacgcag ttggcccgca aactggatgc
    34561 agtagtagaa gactgcgtaa acgccgttgg cgtcgatctc aacactgctt ctgttccgct
    34621 gttaactcgc gtggcgggcc tgacgcgcat gatggcgcaa aacatcgttg cctggcgcga
    34681 tgagaacggg cagttccaga accgtcagca actgttaaaa gtgagccgtc tggggccgaa
    34741 agccttcgaa cagtgtgcgg gcttcttgcg cattaaccat ggtgataacc cgctggacgc
    34801 ctctaccgtt cacccggaag cctatccggt ggtggaacgc attctggcgg cgacgcagca
    34861 ggcactgaaa gatctgatgg gtaacagcag cgagctgcgt aacctgaaag cgtctgactt
    34921 tactgatgag aaattcggtg tgccgacagt aactgacatc atcaaagagc tggaaaaacc
    34981 aggtcgcgat ccgcgtccgg aatttaaaac cgctcagttt gccgatggcg tcgagacaat
    35041 gaacgacctg caaccgggga tgatcctcga aggtgcggtg accaacgtca ccaattttgg
    35101 tgcttttgtt gatattggcg tacatcagga cggcctggtt cacatctctt cattgtcgaa
    35161 caagtttgtg gaagatccgc ataccgtggt gaaagcgggc gatattgtga aggtgaaagt
    35221 gctggaagtg gatcttcagc gtaaacgtat cgccctgact atgcgtctgg acgagcagcc
    35281 tggcgaaacc aacgctcgtc gcggcggcgg gaatgaacgt ccgcaaaaca accgcccggc
    35341 agccaaaccg cgtgggcgtg aagcgcagcc tgccggtaac agcgcgatga tggatgcgct
    35401 ggcggcggca atgggcaaaa aacgttaaac gccctgatag acaaacgtaa acctgccaga
    35461 taaggcgttc acgccgcatc tggcaacgaa cgccttgcct gacataaaag tgccggagaa
    35521 tatctccggc ctttttattc cacaaccaaa ctctcaatat accccgttaa tatttatcac
    35581 ttcattaaca actgaaacct taattaaaca ttaaccagtc ctggtaattc gctattcgaa
    35641 ttatattttc gctgcgatat aaccttgagc cacatcaaca ttgagtcaga ttattattca
    35701 aaccaacatt cgcacacatt ttaagtattg ctgatagaaa ccattctcat tatcattgtg
    35761 ttgttgatta tttaatctcc cctttgttgg caaatcatct ggtctcatgt cgctgtcaaa
    35821 cgccccatga ggtagttatc cagttaatga gaaacaagta ggcacctatg caatacactc
    35881 cagatactgc gtggaaaatc actggctttt cccgtgaaat cagcccggca tatcgccaaa
    35941 aactgctttc tcttggcatg ttacctggct cctcttttaa tgtggtgcgc gtcgctccac
    36001 tcggcgaccc cattcatatc gaaacccgtc gtgtgagcct ggtattacgc aaaaaagatc
    36061 tggccttatt agaagtggaa gcggtttcct gttaatacgg tgataacaac aatgaaaaaa
    36121 ttaaccattg gcttaattgg taatccaaat tctggcaaga caaccttatt taaccagctc
    36181 actggtgcac gtcagcgtgt aggtaactgg gctggcgtta ccgtcgaacg taaagaaggg
    36241 caattctcca ccaccgatca tcaggtcacg ctggtggacc tgcccggcac ctattctctg
    36301 accaccatct catcgcagac ctcgctcgat gagcaaatcg cctgtcacta tattctgagt
    36361 ggcgacgccg acctgctgat taacgtggtg gatgcgtcta accttgagcg taacctgtac
    36421 ctgacgctgc aactgctgga actcggcatt ccctgcatcg tggcactgaa catgctcgac
    36481 attgccgaga agcaaaatat tcgtattgaa attgatgctc tgtcggcgcg tcttggctgc
    36541 ccggtgatcc cgctggtttc aacccgtggt cgcggtattg aagcgctcaa gctggcgatt
    36601 gatcgctata aagctaacga gaatgtggaa ctggtgcatt acgcacagcc gctactcaac
    36661 gaagcagatt cactggcaaa agtgatgcct tccgacatcc cgctgaaaca acgtcgctgg
    36721 ctgggcctgc aaatgctgga aggcgatatc tacagccgcg cctacgccgg tgaagcgtcg
    36781 cagcatctgg atgccgccct cgcccgtctg cgtaatgaga tggacgatcc ggcgctgcac
    36841 attgccgatg cgcgttacca gtgcattgct gccatctgtg atgtggtaag caacaccctg
    36901 acggcagaac ccagccgttt caccactgcg gtagataaaa tcgtgctcaa ccgtttcctc
    36961 ggtctgccga ttttcctctt tgtgatgtac ctgatgttcc tgctggctat caacatcggc
    37021 ggggcgttac agccgctgtt tgatgtcggt tccgtggcgc tgtttgtgca tggtattcag
    37081 tggattggct acacgctcca cttcccggac tggctgacta tcttcctcgc ccagggcctg
    37141 ggtggcggta ttaacacagt gctgccactg gtgccgcaga tcggcatgat gtacctgttc
    37201 ctctccttcc ttgaggactc cggctatatg gcgcgtgcgg cgtttgtgat ggaccgcctg
    37261 atgcaggcgc tgggcttgcc ggggaaatcc tttgtaccgc tgatcgtcgg cttcggttgt
    37321 aacgtaccat cggtaatggg cgcacgtacg cttgatgcac cgcgtgaacg cctgatgacc
    37381 atcatgatgg caccgtttat gtcctgcggc gcgcgtctgg ctatcttcgc agtattcgcg
    37441 gctgccttct tcgggcagaa cggtgcgctg gcggtcttct cgctgtatat gctcggtatt
    37501 gtgatggcgg tgctgactgg cctgatgctc aagtacacca tcatgcgcgg tgaagcgacg
    37561 ccgtttgtca tggagctgcc ggtctaccat gtaccacacg ttaaaagcct gattatccag
    37621 acctggcagc gtctgaaagg cttcgttctg cgtgctggta aagtgattat catcgtcagc
    37681 attttcctga gtgctttcaa cagcttctct ctgagcggga aaatcgtcga taacatcaac
    37741 gactcggcgc tggcttccgt cagccgggtg atcaccccag tcttcaagcc gattggcgtg
    37801 catgaagata actggcaggc aacggttggc ctgtttacag gcgcaatggc gaaagaagtg
    37861 gtggtgggta cgctcaacac cctctacacc gcagaaaata ttcaggacga agagttcaat
    37921 ccggcggaat ttaacctcgg tgaagagctg ttcagtgcgg tagatgaaac ctggcagagc
    37981 ctgaaagaca ccttcagcct tagcgtactg atgaacccga ttgaagcgag caaaggcgac
    38041 ggcgaaatgg gtaccggggc gatgggtgtg atggatcaga aattcggtag cgcagctgcc
    38101 gcatacagct acctgatttt cgtcctgctg tacgtaccgt gcatctcggt gatgggtgct
    38161 atcgcccgtg aatcaagccg tggctggatg ggcttctcca tcctgtgggg gctgaatatc
    38221 gcttactcgc tggcaacatt gttctatcaa gtcgccagct acagccagca tccaacttac
    38281 agcctggtgt gcattctggc ggttatcctg tttaacatcg tggttatcgg tttgctgcgt
    38341 cgcgcgcgta gccgggtgga tatcgaactg ctggcaacgc gcaagtcggt aagcagttgc
    38401 tgcgcagcca acaccaccgg tgattgccat taatggcttc acttattcag gtgcgtgatt
    38461 tgctggcgtt acggggccgt atggaagcgg cccagataag ccagacattg aacaccccgc
    38521 agccaatgat taacgccatg ctgcaacagc tggaaagcat gggcaaagcc gtgcggattc
    38581 aggaagaacc tgacggctgc ctctctggca attgtaaaag ctgcccggaa ggcaaagcct
    38641 gcctgcgcga gtggtgggcg ctgcgttaac catactccat cacctgtttt gaaaagcccg
    38701 gtatgcgttt gcatccgggc ttttttgcgt gcgactttcc ataaaaatgt aacccttgca
    38761 gcacggcgca agttcctctg aaagcgtctc gcagggatga aaactcgcta atgcacaggt
    38821 gtggagtggc gcgtagagtc gcggcattca aacaacaggt gaaggaacgc catgagcaaa
    38881 aagcagagtt ccaccccaca cgatgcgctg ttcaaactct ttttacgcca accggagacg
    38941 gcgcgcgatt ttcttgcgtt ccatttaccg gcacctattc acgcgctctg tgatatgaaa
    39001 accctcaagc tggagtcgag cagctttatt gatgacgatc tgcgtgaaag ctattccgac
    39061 gtgctgtggt cggtgaaaac ggaacaagga ccaggataca tctattgtct gattgaacat
    39121 caaagcacct caaacaaact gatcgcattt cgcatgatgc gctacgccat tgccgcaatg
    39181 caaaatcacc tggatgccgg atacaaaacc ttgccgatgg tggtgccatt gttgttttac
    39241 cacggtattg aaagccccta tccctattcg ctgtgttggc ttgattgttt cgccgatccc
    39301 aacctggcaa ggcagcttta tgcctctgca tttccgctga ttgatgtcac cgtcatgcct
    39361 gatgatgaaa tcatgcagca ccgacgtatg gcactgctgg agttaattca aaaacatatt
    39421 cgtcaacgcg acctgatggg gctggtagag caaatggcct gcttattaag tagtggatac
    39481 gctaatgaca gacaaatcaa agggctgttt aattacatac tgcaaactgg cgatgcggta
    39541 cgttttaacg attttatcga cggtgtggcc gaacgttcac cgaaacacaa ggagagttta
    39601 atgactattg cggaaagatt gcggcaggag gggtttcaga aaggtatacg taccaacgcc
    39661 ctaaatattg ccaaaaccat gctggatgcc ggagtctctc ttgaggacgt tttgcgattt
    39721 accagcctga cggttgaaga ccttgctgaa ataaatcatc aataacgtaa aggatggttt
    39781 taatgacaga tgcagaaaga gaatatctta ttagttacct gattggttat ttattgggtc
    39841 gacgcctgga agccctggat atcgccaaaa gaatgctgta atccggagtc cctcttgcgg
    39901 acatcatgcg ttttaccggg gtgtcagaag aagagttggc tgcggcgagt cagtaaaatt
    39961 ctgtctcgcc atttcaaaag ctgcctacac cctctgcttc aacgccacca gcacgtgacg
    40021 aaactcgact ggatgcgaaa taaacggcgc atgggccgct ttggcgaaaa tgtacgactc
    40081 gctgtgcggc cagagtttat ccagcatcgg caccactttg cgcggcacca gaccatcgag
    40141 ataaccatac aatcgcaaaa acggcatgga cacgttttgc agcgactgac ggagatcgac
    40201 cgttttcagg atttccagcc cgccattaag cacgtcaacc tccggcatcg gtaacgccag
    40261 aacggttttc ttcagcgccc gcgcatcctg gcgggccgtt tcagtaccca tggtttgtaa
    40321 cgccaggaac cgttccactg tacgctgaaa atcatcactg agttgctgct gaaatcccgc
    40381 cagcacgtcc ggttttatcc ccggccactc gtcacgagca ctaaaacaag gtgacgacgc
    40441 cacggtgacc agtgcctgaa cacgctcggg atgggttaac gcaatctggc ttgccaccag
    40501 cccgcccaga ctccagccta accaaatggc tttatcaggt gcctgtcgca gcacggcttc
    40561 ggccatatca gcaagtgaca tcgcaccaaa tccccgactg cgcccgaaac cgggcagatc
    40621 aacaaggtgc agcgtaaaat gcgagctaag ttcctcgtca atgcaacgcc acacttcggc
    40681 attcagtccc catccgtgca gcagcacaag atgaacattc ccctgacctt tggtctgcca
    40741 ccagatgtta ttcatccgct attgttctct tttgacttac aaggatgaac atatgctaac
    40801 agtaccggga ttatgctggc tatgccgaat gccactggcg ttaggtcatt gggggatttg
    40861 ttcggtctgt tcacgcgccg cccgtacaga taaaacgtta tgcccacaat gtggattacc
    40921 cgccacacac acccatcttc cctgcggtcg ctgcctgcat aaaccaccgc cctggcaaag
    40981 actggtcacg gttgccgact atgcgccgcc gttaagtccg cttatccacc agcttaagtt
    41041 ttcccggcgc agcgaaatcg ccagcgccct gtcacgtctg ttactattgg aagtcttaca
    41101 cgctcgtcgc accaccgggt tgcaattgcc ggatcgcatc atcagcgttc cgttatggca
    41161 gcgacgtcac tggcgtcggg gatttaatca gagcgatttg ctgtgtcagc cgttatcacg
    41221 ctggttgcac tgccgatggg atagcgaagc cgtcacacga acacgggcca ctgcgaccca
    41281 gcattttctc agtgcccggc tgcgcaagcg caacctgaaa aatgcctttc gccttgaatt
    41341 gcctgtgcaa ggtcgccata tggtgattgt ggatgatgtc gtcaccaccg gaagtaccgt
    41401 cgcagagatt gcgcagttgc ttttacgcaa tggtgcggcg actgtccagg tctggtgcct
    41461 ttgtcgaacc ttgtagagcc tcgatgatgg gcgtattata accaactaaa atagtcaact
    41521 attaggccat tactatgatc cgtatttccg atgctgcaca agcgcacttt gccaaactgc
    41581 tggcaaatca ggaagaaggg acacaaatcc gcgtatttgt gattaaccct ggcacgccta
    41641 acgctgaatg tggcgtttct tattgtccgc cggacgctgt agaagccacc gacactgccc
    41701 tgaaatttga cctgctgacc gcgtatgttg atgagttaag cgctccttac ctggaagatg
    41761 cagagatcga ttttgttacc gaccagttgg gttctcagct aacgctgaaa gcgcctaatg
    41821 ccaaaatgcg taaagtagca gacgatgcac cgctgatgga gcgcgtggag tatatgctgc
    41881 agtcgcagat caacccacag cttgctggtc acggtggtcg cgtttcgctg atggaaatca
    41941 ccgaagacgg ttacgccatt ctgcaatttg gcggcggctg taacggttgt tccatggtcg
    42001 atgtgacgct gaaagaaggg atcgagaagc agctgctaaa cgaattcccg gagctgaaag
    42061 gtgtgcgcga tctcaccgaa caccagcgcg gcgaacactc ctactactaa gttatcccct
    42121 cttttgtaga ttgcccgatg cgacgctaaa gcgtcatttc gggcctacgt tgaacatgtg
    42181 ccagacgttg gcgcagcatt gctttggcaa atccctccct tcgtttcctc gcgataatat
    42241 gaccaacctc tcataattta aatttacccc gctctggtga ttctcaaacg ccagatgtta
    42301 cccgtatcat tcacatgggt accaaacata ctcctgacat ctgactacaa taattagttt
    42361 tagtgggtat cagtcgtggt gccgcaatat ctctgttccc gattgggata attagagttt
    42421 gtcgtcagaa aattgacgtt acccataaca aatgaaaggc caggtaaatc atgccattag
    42481 tcattgttgc tatcggtgta atcttgttgt tgctcctgat gatccgcttc aaaatgaacg
    42541 gcttcatcgc tctcgtcctc gtggcgcttg ctgttggatt aatgcaagga atgccgctgg
    42601 ataaagttat tggctccatc aaagccggtg tcggcgggac gctcggtagc cttgccctga
    42661 tcatgggttt tggcgcaatg ctgggcaaaa tgctggcaga ctgtggtggc gcacaacgta
    42721 tcgccaccac gctgattgct aaatttggta aaaaacacat ccagtgggcg gtggtactga
    42781 ccggttttac cgttggtttt gccctgttct atgaagtggg tttcgtgctg atgctgccgc
    42841 tggtgtttac catcgcggct tccgcgaata ttccactgct gtatgttggt gtaccaatgg
    42901 cggctgcact gtctgtgacc cacggcttcc tgccaccgca tccgggtccg actgcgattg
    42961 ccaccatttt caatgccgat atgggtaaaa ctctgctgta cggtactatt ctggcaatcc
    43021 cgaccgtgat tctcgccggt ccggtttacg ctcgcgtgct gaaaggtatc gataagccga
    43081 tcccggaagg tctgtacagc gcgaaaacct tcagcgaaga agagatgccg agttttggcg
    43141 taagcgtctg gacctctctg gtcccggttg tgctgatggc gatgcgtgcg attgccgaaa
    43201 tgatcctgcc gaaaggtcac gctttcctgc cggtagcgga gttcctcggt gacccggtaa
    43261 tggctacgct gattgccgta ctgattgcga tgttcacctt tggtctgaac cgtggtcgtt
    43321 caatggatca gattaacgac acgctggttt cttccatcaa aatcattgcg atgatgctgt
    43381 tgattatcgg tggtggcggt gcgttcaagc aggtgctggt agacagcggc gtggacaaat
    43441 acattgcttc catgatgcac gaaaccaaca tttctccgct gctgatggcc tggtcgattg
    43501 ctgccgtact gcgtatcgcg ctgggttccg caaccgttgc ggcaatcact gcgggtggta
    43561 tcgcggcacc gctgattgca acgacgggtg ttagcccgga gctgatggtt attgcggttg
    43621 gttccggtag tgtgattttc tctcacgtga acgatccggg cttctggctg ttcaaagagt
    43681 actttaacct gactatcggc gagaccatca agtcctggtc gatgctggaa accattatct
    43741 cggtgtgcgg tctggtaggc tgtctgctgc tgaatatggt gatttgataa caccgactgc
    43801 cggatgcatc gcatccggca tcaaccgcac cctactactt cttcgctgcc gctctgcgcc
    43861 gtctgtccaa atccttcagc aacttgttca cgccatcatc ggcaaacatc gcctcaagcg
    43921 ttgcagagag cttgcgccgc cagtttttat actggtaact ggtgccggga atatttaccg
    43981 gttccgccat atccagccag tcttctggct gtagccctaa cagggcactg ttactgtcag
    44041 cgatataacg ttgcaaacca cgattcagcg tcggcgtcat cgacatcaac gatgccttat
    44101 gcccggcacg ttttggcagg caaccatatt tatgcagagc atccagcagt ccttgcttcg
    44161 ccagttcgcg atcctgatac agaccgcgca gtaccacttc atccggatac aaccccagcg
    44221 ttttgcccag cgttagatcc ccgctctccc agtagccgcg tagcgtaggc aggtcgtgtg
    44281 tcgccgcaac cgccatcgac tgctctgggt acgctttcgg tgagcggaat gtcttctcgt
    44341 ggtcgttttc gaaatagagc actttgtagg agtacacacc gctgctgcgc agcttaccga
    44401 caatctctac cggtacggta ccgagatctt caccaatcac catgcagcga tgacgtttac
    44461 tttcgagtgc cagaatcgag agcagatcat ccaccggata gtgtacatac gcgccctgat
    44521 ccgccgtctc gccatacggt atccaccaca aacgcagcat cgacatcaca tggtcaattc
    44581 gtaatgcgcc gcagttttgc atattggcac gcaacagctc gataaacggt tcataggcac
    44641 gcgcggtgat gatatgcggg tccattggcg gcaatcccca gttctgcccc aacgggccga
    44701 ggatatccgg cggtgcgcct accgacgctt tcaggcaata cagttcacgg tcgcaccagg
    44761 tttccgcccc accttccgct acgccaaccg ccagatcacg atacaagcca atcggcattt
    44821 catagccctg gcttatctcc cagcagtcgg caaactggct gtaagccagc cactgcaacc
    44881 agagataaaa atcgacgtca tcacgatgtt cttcgcagaa ctggcgcact tctggtgaat
    44941 ccacgttctg atacatctct ggccatgcag gccagcccca gcgcatttcg tcctctttca
    45001 cttgctgggc atgtagtgcg tcaaacgccg cctgccagaa caggctgtcg ccttgctcgg
    45061 caacaaactg gcgaaacgcg gtcatctgtt catcatcacg ttgcgcgaaa cctttccacg
    45121 ccattcgtaa tgctgtcatt tttagggcgg taaccgtgga gtaatcaacc cagtcggcat
    45181 cccgcgcctg ttgcagcgtc tgttgcgtgg tcggcaactt ccaccaggcc tgagcctctt
    45241 cgctaagatg gaaatcttcg acggcgttaa cgtcgatata aatcacattc agccaacggc
    45301 gagaagacgg gctatacggg ctggcacttt ccggatttgc cggatagagc gcatgaatcg
    45361 ggttcagacc gatgaacgac ccgccacgtt ttgccacatc ctcaagcatc gctttgagat
    45421 cgccaaaatc cccaataccc cagttttttt ccgatcgcag cgtataaagc tgaacgcagg
    45481 caccccacag cttttgttta ttcagcaacg cctgcggttc gtaacagcgt ttcggtgcca
    45541 caatcacccg gcaatgcgcg cgctggtcgt cctgggtgag tgtcagcgtg tgataacctt
    45601 ccggcagctt cgtcggtaga ttgaacgctt tgccccccgt tacatggcct ttgtactgcg
    45661 ttccttcttc ggtggtcagc agccagctat attcgccgct gccctccacc accatcggca
    45721 tttttttgcc gctggtataa accatgacat tcgggactgg cgttaccgcc actttcgtgg
    45781 cggtacgttg atgcatcgcg tcaagcaaac gccgtttggt ttcggcgcta atcgactgcg
    45841 gtttaccgtg ggcattgatg taattggggc taatccccgc cgccagcgcg gcattatcca
    45901 gacgtttgct ttccatcgag cttccttagc gttttgcctg ccagatacga gcctgataat
    45961 cgcgaataga gcgatccgag ctaaacatac cgcagcgggc ggtattgagg atcgccgcgc
    46021 gggtccaggc ctcctggtcg cggtacagca catccacctg cttttgtgcc tctacataag
    46081 cggcgaaatc tgccatcacc agatacggat cgccgccctg tttgccgata ctgtgcagca
    46141 tctggtcgaa ggcatgctta tcgccgtcgc tgtatttacc gctttccagc tctttcaata
    46201 ctgcgtccag caccttatct ttcttccgcc atttcaccgg gtcgtagcct ttggccagaa
    46261 ttgccttcac ttgttccacg gtatgaccaa aaataaagat attttcttca ccgactttct
    46321 cagcgatttc aacgttcgca ccatccagcg taccgacagt aagcgcacca ttgagcgcca
    46381 gtttcatatt gccggtaccg gaagcttctt tacctgccgt cgaaatttgt tcggagatat
    46441 ccgccgccgg gatcagtttt tccgccgccg aaacacaata atccggcaag aacaccacct
    46501 tcaacttgtc gccaaccagc ggatcgttgt tgatcacgtc agccactttg ttgatcgcaa
    46561 agataatgtt tttcgccagg tagtagcccg gtgccgcttt cgcgccaaag aggaagacgc
    46621 gcggtacgcg atcagcctgc gggttttcac gaatttcttt gtacaacgcc agaatatgca
    46681 gcagattcag gtgctggcgt ttgtactcat gcagacgttt gatctgaata tcgaaaatcg
    46741 cctgtggatt gatctcaata ccggtacgaa ctttcacaaa ctctgccaga cggactttat
    46801 tcgcctgctt gatctcgcga tattgctgac ggaatttcgc atcatcagcg aatttttcca
    46861 gattgatcag ttgatcgaga tcgttagccc actctttttt cagtgattta tccaacagag
    46921 ccgccagtgc cgggttgcac tgtttgatcc agcgacgtgg ggtaataccg ttggtgacgt
    46981 tatggaattt gttcggccat agctggtgat attccgggaa cagatctttc actaccagat
    47041 ccgagtgcag cgccgcaaca ccgtttaccg cgaaaccgcc aactacacac aggttcgcca
    47101 tatgcacttg tttgtcgtgt accaccgcca gtttggccca tactttctca tcgcccggcc
    47161 aggttttttc caccagcgtt ttaaagcgtg tattaatttc gttaataatc tgcatgtggc
    47221 gcggcagtaa gcctttcacc agtttcacat cccagcgttc cagcgcttct ggcatcaggg
    47281 tatggttggt gtaagcgaat gttttgctgg taatggccca ggcgtcatcc cagctcatct
    47341 ggtgctcatc gatcagcacg cgcagcagtt ctggaatcgc gatagttggg tgggtatcgt
    47401 tcagctgaat aacttcataa tccgccagtt cgtgcagttt acgccccgcc agatgatggc
    47461 gacgcaaaat atccgctacc gagcaggcac actggaagta ttgctgcatc aggcgcagct
    47521 ttttaccggc agtatggttg tcgtttggat agagaacttt ggtcagtttt tccgcgttga
    47581 tgccctgctg ttcggcacgc aggaaatcac cgtcattaaa tttagtcaga tcaaacggat
    47641 gtgcatgcat cgcctgccac agacgcagcg gctgcgccac gccattacga tagccgacaa
    47701 cggggagatc ccacgcttga ccggtaatgg taaactccgg ctcccagcgt ccgtctttcg
    47761 tcactttacc gccaatccct acctgcacat ccagtgcttc gttgtggcgg aaccacgggt
    47821 agttactgcg atgccagtca tccggcgctt caacctgttt gccatcgaca aaagactggc
    47881 ggaacaaacc atattgatag ttcagaccgt aacccgtcgc agactgaccg acagttgcca
    47941 ttgagtcgag gaagcacgcc gccagacgtc ccagaccacc gttacccagc gccgggtcaa
    48001 tctcttcttc cagcaagtca gtcaggttga tgtcataagc cttcaacgaa tcctgtacat
    48061 cctgatacca gccgagattc aacaggttgt tgcccgtcag gcgaccaatc aaaaactcca
    48121 ttgagatgta gttaacatgc cgctgattcg ccaccggttt ggcaaatggc tgagcacgca
    48181 gcatttcggc cagtgcttcg ctcactgcca gccaccactg acgaggggtc atttcagccg
    48241 cagaatttaa gccataacgc tgccactgac gtgaaagcgc ttcctgaaat tgcttatcgt
    48301 taaaaatagg ttgtgacata ggagttccac ttttcttaga ttttcaacac aacgttatcg
    48361 ctagtttgcc aggctcgatg ttgaccttcc tcatcctgcg ggggattagg cagggaggag
    48421 ttgcggggat gagcaaggaa atgtgatctc gaccacttaa agctagtgca aaccacagga
    48481 ttagcagcaa atcaatgcca tacagcgcag aaaatctgta tctaagtgca aaaaatggcc
    48541 gttgcgtatt ttcaaaaagc ggaaggtaac tctataaatt aagtaaagga gtgaaacagt
    48601 ttcataagta aaatatcaag tgtgctccat ctcattctta atagatttat taagatcatc
    48661 tttttagatg gcactttcat cagggataaa gaaaaaaccc ttgcttaaat gaaactgatg
    48721 aacataaggg aaataccgta caacccagca ttcacgctgt atcagcgtcg ttttaggtga
    48781 gtcattaata aagatttgga attgtgacag agtgcaaatt cagacacata aaaaaacgtc
    48841 atcgcttgca ttagaaaggt ttctggccga ccttataacc attaattacg aagcgcaaaa
    48901 aaaataatat ttcctcattt tccacagtga agtgattaac tatgctgatt ccgtcaaaat
    48961 taagtcgtcc ggttcgactc gaccataccg tggttcgtga gcgcctgctg gctaaacttt
    49021 ccggcgcgaa caacttccgg ctggcgctga tcacaagtcc tgcgggctac ggaaagacca
    49081 cgctcatttc ccagtgggcg gcaggcaaaa acgatatcgg ctggtactcg ctggatgaag
    49141 gtgataacca gcaagagcgt ttcgccagct atctcattgc cgccgtgcag caggcaacca
    49201 acggtcactg tgcgatatgt gagacgatgg cgcaaaaacg gcaatatgcc agtctgacgt
    49261 cactcttcgc ccagcttttc attgagctgg cggaatggca tagcccactt tatctggtca
    49321 tcgatgacta tcatctaatc actaatccag tgatccacga gtcaatgcgc ttctttattc
    49381 gccatcaacc agaaaatctc accctggtgg tgttgtcacg caaccttccg caactgggca
    49441 ttgccaatct gcgtgttcgt gaccaactgc tggaaattgg cagtcagcaa ctggcattta
    49501 cccatcagga agcgaagcag ttttttgatt gccgtctgtc atcgccgatt gaagccgcag
    49561 aaagcagtcg gatttgtgat gacgtttccg gttgggcgac ggcactgcag ctaatcgccc
    49621 tctccgcccg gcagaatacc cactcagccc ataagtcggc acgccgcctg gcgggaatca
    49681 atgccagcca tctttcggat tatctggtcg atgaggtttt ggataacgtc gatctcgcaa
    49741 cgcgccattt tctgttgaaa agcgccattt tgcgctcaat gaacgatgca ctcatcaccc
    49801 gtgtgaccgg cgaagaaaac gggcaaatgc gcctcgaaga gattgagcgt caggggctgt
    49861 ttttacagcg gatggatgat accggcgagt ggttctgcta tcacccgctg tttggtaact
    49921 tcctgcgcca gcgctgccag tgggaactgg cggcggagct gccggaaatc caccgtgccg
    49981 ccgcagaaag ctggatggcc cagggatttc ccagcgaagc gattcatcat gcgttggcgg
    50041 caggcgatgc gctgatgctg cgcgatattc tgcttaatca cgcctggagt ctgttcaacc
    50101 atagcgaact gtcgctgctg gaagagtcgc ttaaggccct gccgtgggac agtttgctgg
    50161 aaaatccgca gttagtgtta ttgcaggcgt ggctgatgca aagccaacat cgctacggcg
    50221 aagttaacac cctgctagcc cgtgctgaac atgaaatcaa ggacatcaga gaaggcacca
    50281 tgcacgcaga atttaacgct ctgcgcgccc aggtagcgat taacgatggt aatccggatg
    50341 aagcggaacg gctggcaaaa ctggcactgg aagaactgcc tccgggctgg ttctatagcc
    50401 gcattgtagc aacctcagtg ctgggtgaag tattgcactg caaaggcgaa ttgacccgct
    50461 cactggcgtt aatgcagcaa accgaacaga tggcgcgcca gcacgatgtc tggcactacg
    50521 ccttatggag tttaatccag caaagtgaaa ttctgtttgc ccaagggttc ctgcaaaccg
    50581 cgtgggaaac gcaggaaaaa gcgttccagc tgatcaacga gcagcatctg gaacagctgc
    50641 caatgcatga gtttctggtg cgcattcgtg cgcagctgtt atgggcctgg gcgcggctgg
    50701 atgaagccga agcgtcggcg cgtagcggga ttgaagtctt gtcgtcttat cagccacagc
    50761 aacagcttca gtgcctggca atgttgattc aatgctcgct ggcccgtggt gatttagata
    50821 acgcccgtag ccagcttaac cgtctggaaa acctgctggg gaatggcaaa tatcacagcg
    50881 actggatctc taacgccaac aaagtccggg tgatttactg gcaaatgacc ggcgataaag
    50941 ccgccgctgc caactggttg cgccatacgg ctaaaccgga gtttgcgaac aaccacttcc
    51001 tgcaaggtca atggcgcaac attgcccgtg cacaaatctt gctgggcgag tttgaaccgg
    51061 cagaaattgt tctcgaagaa ctcaatgaaa acgcccgcag tctgcggttg atgagcgatc
    51121 tcaaccgtaa cctgttgctg cttaatcaac tgtactggca ggccggacgt aaaagtgacg
    51181 ctcagcgcgt gttgctggac gcattaaaac tggcaaatcg caccggattt atcagccatt
    51241 ttgtgattga aggcgaagcg atggcgcaac aactgcgcca gctgattcaa ctcaatacgc
    51301 tgccggaact ggaacagcat cgcgcacagc gtattctgcg agaaatcaat caacatcatc
    51361 ggcataaatt cgcccatttc gatgagaatt tcgttgaacg tctgctaaat catccggaag
    51421 tgccagaact gatccgcacc agcccgttga cccaacgtga atggcaggta ctggggctga
    51481 tttactccgg ttacagcaac gagcaaattg caggcgagct ggaagtggcg gcaaccacca
    51541 tcaaaacgca tatccgcaat ctgtaccaaa aactcggtgt cgcccaccgt caggccgcgg
    51601 tacaacacgc gcagaaactg ctgaagatga tgggatatgg ggtatgagtt cagccggata
    51661 ttacgtcgga tccggcttac atctttatat cattcaatgc tcacccgcgt tacgccatca
    51721 gcctccacca gactaaaccg cactggcaag aaacgctcca ccaccgcgat attggtcagc
    51781 agatggcatg agggatgggc gaccgtaaat tctcccgcgc ccgccagcgc catcggtagc
    51841 accagctggt cggcgagata ttcccccact gccgccgggc ttgccaggta gcgtttcacc
    51901 tctttcacta actgcgcggc taccacttcg gcactgacgc gcttttcacc gacgacgaaa
    51961 aagcgttcag tgatattttc actttcgact tccagcgaga cggtattacc cggcccctgg
    52021 tcgcgcggca ggctatgaat attctgctca tgcagggaaa aacttgccac cagcgtagcg
    52081 atttcacgct cagcaacatg cctcggcacg ccagctaata gcacctctcc acgcatctgc
    52141 acaatgttcc cgcgctcgcc aagttgcaag gtgttaaaca atgccacggg cgagacttcc
    52201 gctgccacca ccccgcctcc agcaggataa aaaccgtggc gtaataatgt ggtttgctga
    52261 tgaatgccca ttttcgccag cagcggctcc agcacccgac agataaaatc cgcaggcggt
    52321 gccgacgggt tatcggtgcc accgctcact tcaacacgcg aaggtccatc agcaaaccac
    52381 agcgcgggca gcaccgtttg cagtaccagc gtacaacttc cggcgctacc gatagcaaag
    52441 cggtaatcgc cgccgtgcac ggtacctggc cggaagacca gacgctgcga ccctagctcc
    52501 gcgccttcca ccgttgccct acaaatttcc gcagccgctt ttaccgcggt cagatgctgg
    52561 cgcaacagcc ccggtttcgc ccgcccggca cgaatgccag tgatggtaaa tggctggcca
    52621 gttatcatcg acaggctcag cgccgagcgc aggatctgcc cgccgccttc gccctgtgcg
    52681 ccatccagcg caatcatcct tttcatcatt tatcctttta cgcacaccac ctgacgcagg
    52741 gtatagataa cttccaccag atcgctttgt gccgccatca ccgcatcaat atctttatac
    52801 gccatcggga tttcgtcgat cacttcggca tctttacggc attccacatg cgcggtggca
    52861 cgaatttgat cttccacgct gaacagtttt ttcgctttgg tacggctcat tacccgcccg
    52921 gcaccgtggc tgcacgaaca gaacgattct tcatttccca gcccacggac gataaagctt
    52981 ttcgctccca tcgaccctgg aataattcca tattgacccg cacgcgcaga caccgcgcct
    53041 ttacgcgtca cgtagatctc ttcaccaaag tgctgttctt tttgcacata gttgtgatga
    53101 cagttgatct cttccatcgc cagcgtttgt ggctgtctta ccgttttctg cgtaacgctc
    53161 tgcaacgccg ttaccacgtt ttccatcatc gcatcgcggt taaggctggc aaaaagctgc
    53221 gcccaggcca cggctttcag gtaatcatca aagtattccg taccttccat aaagtacgcc
    53281 agatcacgcg acggcaacgt ctcaagttgg tcctgcatct ctttttgcgc cagatcgata
    53341 aagtaagtcc cgatggcgtt accaattccg cgtgaaccgg agtgcagcat aatccacacc
    53401 tggtccgact catcaaggca gatttcaata aagtggttac ccgttcccag cgttcccagg
    53461 tgtttatagt tattagtgtt aaggaaacgc ggatattttt gcgttaacca ctgataaccg
    53521 acttccagct cagtccattt cgcatcgacg ttaacaggcg ggttttccca tgcgccttta
    53581 tcacgtttac aacggccagt agtacgtccg tgcggcacgg ccgtttcaat cgcctgacgc
    53641 agctccgcca ggttttcagg caggtctgcc gccgttaacg cggtacgcag cgcgttcatt
    53701 ccacaaccga tatccacgcc caccgccgcc ggaataatcg cccctttggt cgggatcacg
    53761 ctaccaatgg tggaaccttt acccaggtgt acatcaggca ttaccgcaat atgtttgaaa
    53821 ataaacggca tcttcgccgt attaataagt tgctgacgcg catcggcctc taccggcacg
    53881 cctttggtcc acatttttac cggggcattt tcagtggtca gtaattcgta attcattttg
    53941 tttttctctt ttcgttgttt gctgtcctga taattgcaac cgtcgtgcca gaaaattaat
    54001 taagcagctt aattttttaa ttcattgttt ttaatagatt aaatctttac gtccgtaaac
    54061 ggagatttcc cgcaaagcca atttaccgat aatgaaatat cgtcttttat aaggatatct
    54121 aagatgcgta aaacagtggc ttttggtttt gtcggaacgg tgctggatta tgccgggcgc
    54181 ggcagtcagc gctggtcaaa atggcgtccg acactctgtc tatgccagca agaatcgtta
    54241 gttatcgatc ggctggaatt attgcacgac gcccgctcgc gctcgctatt cgaaacgctt
    54301 aaacgcgata tcgccagcgt ttcgtcagaa acagaagtgg tgggcgttga gatagaactg
    54361 cataacccgt gggatttcga agaggtttac gcctgcctgc atgatttcgc ccgtggttac
    54421 gcgttccagc cagaaaaaga agactattta attcacatca ccaccggtac ccacgtcgcg
    54481 cagatttgct ggtttctgct ggcagaagcg cgttatctac ccgcccggtt gatacaatct
    54541 tcaccaccgc gcaaaaaaga acaaccgcgc ggcgcaggtg aagtgacgat tatcgatctc
    54601 gatttaagcc gttataacgc catcgccagc cgctttgccg aggaacgcca gcaaaccctc
    54661 gacttcctca aatccggcat tgccacacgc aacccccact tcaaccgcat gattgagcag
    54721 atcgaaaaag tggcgatcaa atcccgcgca ccgatcctgc ttaacggccc aaccggtgcg
    54781 ggcaagtcgt ttctggcacg acgcatcttt gaattgaaac aggcgcggca tcagtttagc
    54841 ggcgcatttg tagaagtgaa ctgcgccacc ctgcgcggcg ataccgccat gtcgacgctg
    54901 tttggtcatg tgaaaggcgc gttcaccggg gcgcgggaat ctcgtgaagg attattacgc
    54961 agcgccaacg gtggcatgtt gtttcttgat gagattggcg aactgggcgc agacgaacag
    55021 gcgatgttgc tgaaagccat tgaagagaaa accttttatc cgtttggcag cgatcgccag
    55081 gtgagtagcg actttcagct tatcgccgga acggtgcgcg atttgcgtca gctggttgcc
    55141 gaaggcaaat tccgcgaaga tctgtacgcg cggatcaatc tctggacctt caccctgccg
    55201 ggtctactcc agcgccagga agatattgaa ccgaacctgg attatgaagt ggagcgccac
    55261 gcctcactca ctggcgacag cgtgcgtttt aacaccgaag cgcggcgcgc ctggctggct
    55321 tttgcgacct ctccccaggc aacatggcgc ggtaactttc gcgaactttc tgccagcgtc
    55381 acgcggatgg caacctttgc cactagcgga cgcatcactc gggaagtggt ggaagatgag
    55441 ataaaccgtc tgcgctataa ctggcaggag agtcgcccct ccgcgattac ggcgttgctg
    55501 ggcgcagagg cagaaaacat cgatctcttc gaccgtatgc aactggaaca cgttatcgct
    55561 atctgccgcc aggcaaagtc gctttctgct gccggacgac agctttttga tgtttcgcgc
    55621 cagggcaaag ccagcgtcaa tgacgcggat cggctacgca aatacctggc gcgttttggt
    55681 ctgacgtggg aagccgtgca ggatcagcac agctccagtt gaatatgatg gtccgtcagc
    55741 acctgcatca cgcttactgg cggcggggcg tcggtgtaga cggcatctac catgctgata
    55801 ctgcccatat tgaccatcgc gttacggcca aatttcgagt gatcgacaac cagcataacg
    55861 tggcgcgagt tctcaataat ggcgcgtttg gtgcgaactt cgtggtaatc gaactccagc
    55921 agcgagccgt cgctatcgat gccactaatc cccagaatgc cgaaatcaag gcggaactgg
    55981 gagataaaat cgagcgtcgc ttcgcctatg atcccaccat cgcggctacg taattcgcca
    56041 ccggcgagaa taatgcgaaa atcttctttt accatcaacg tgttagcgac gttgagattg
    56101 ttggtgacaa tgcgcaaatt gctgtgatta agcagcgcgt gcgccaccgc ttccggggtg
    56161 gtgccgatat cgataaacag cgtcgagccg ttggggattt gctccgccac tttgcgggca
    56221 atacgctctt tttcttcggt ctgggtggct ttgcgatcgt gccacggcgt gttaaccgaa
    56281 ctggaaggta gcgccgcgcc gccatgatgg cgcaggatca ggttttgctc cgccagctcg
    56341 ttgaggtcgc gacgaatagt ctgcgggctg acggagaagt gctccaccag ttcctcggta
    56401 ctgacataac cttgctgttt aaccagttcg ataataccgt tgtgacgttg tgtttgtttc
    56461 atttataaat ccctggaatt attttcgttt tcgcgcattg agcgaatcaa caaaagccat
    56521 cgctaaaccc acggctaacc cggcgatgtg tgctccgttc gccatcgaca tcccaaacaa
    56581 atcaaaccat ccggcgacaa tccagatcag cgcaaagata attaacccac gttgcaggta
    56641 aatgccactt tgcggatcgc gttcgccacg tagccagacg tagcccatca gcgcatacac
    56701 cacgccagaa agcccgccaa accacggccc gctgaatttt tgctgcacat agccgcttaa
    56761 cagggcgcta atgagagtaa tgacaattag cttaccgcta ccgaggcgtt tttccaccgc
    56821 tccgccgaga taccaccacc agagcaggtt aaagaggata tgcatcagcg agaagtgcat
    56881 taacgcgtgg gtgaagtaac gccagaactc aaatttcagt gtcggatcga atggccaggc
    56941 cagccataac atcacttcct gatcgccgag aatttgcatg gcaataaaca ccaccacgca
    57001 ggcgatcatc atcacccagg ttaccgggcc tgcgcgttca cgcaaggcag caaagaaagg
    57061 ataacggcga taatgcaggc cactgccggt atggcccgcc tgccagctgg ccgccagata
    57121 acgcggatct gccgggtttt cgagaaaacg caccagctcc gcccgcacgc gctcggcctg
    57181 ggactcatcc gccagccaga catcgctttg gttatgttgt tgaatcgtga ggataacacc
    57241 ctgcgtcgcc atgtaatcaa caaacgcctg cgccacgcgg gggttagcaa aagaggtaat
    57301 catcaacatc gttgctgtcg cttattccac acaaaagggg acagtataaa gcgttacgcg
    57361 ccgtacgcca cctctgcggg aaactgacgc tgccaggctt caaagccgcc gtcaatgcta
    57421 tagaccacat cgtagccctg ttgcagcaga tactgcgccg cacctttgct gctattgccg
    57481 tggtaacaca tcaccatcac cggagtgtca aagtcgttat cacgcataaa cgcgcccagc
    57541 gtgtcgttag tcaaatggaa agcctgcacc gcatgcccca tagcgaaact ctgtggatcg
    57601 cgaatatcga ccagcaccgc ctctttttcc tgcaacttct ggtgcgcgtc ggcaacgtta
    57661 atacattcga actgatccat gcgtctctct ttctttacaa acaagtgggc aaatttaccg
    57721 cacagtttac gtcgaagcgg cagataaacg ccataatgtt atacatatca ctctaaaatg
    57781 ttttttcaat gttacctaaa gcgcgattct ttgctaatat gttcgataac gaacatttgt
    57841 gagctttaac gaaagtgaat gagggcagca tggaaaccaa agatctgatt gtgataggtg
    57901 gcggcatcaa tggtgctggt atcgcggcag acgccgctgg acgcggttta tccgtgctga
    57961 tgctggaggc gcaggatctc gcttgtgcga cctcttccgc cagttcaaaa ctcattcacg
    58021 gtggcctgcg ctaccttgag cactatgaat tccgcctagt cagcgaggcg ctggctgaac
    58081 gtgaagtgct actgaaaatg gccccgcata tcgccttccc gatgcgtttt cgcctgccgc
    58141 atcgtccgca tctgcgcccg gcgtggatga ttcggattgg tctgtttatg tacgatcatc
    58201 tgggtaaacg caccagccta ccgggatcaa ccggtttgcg ttttggcaca aattcagtat
    58261 taaaaccgga aattaagcgc ggattcgaat attccgactg ttgggtagac gacgctcgtc
    58321 tggtactcgc caacgcccag atggtggtgc gtaaaggcgg tgaagtgctt acccgtactc
    58381 gtgccacttc tgctcgccgc gaaaacggcc tgtggattgt ggaagcggaa gatatcgata
    58441 ccggcaaaaa atatagctgg caagcgcgcg gcctcgtgaa cgccaccggc ccgtgggtga
    58501 aacagttctt cgacgagggg atgcatctgc cttcgcctta tggcattcgc ctgatcaaag
    58561 gcagccatat tgtagtgcca cgcgtacata cccagaagca agcctacatt ctgcaaaacg
    58621 aagataaacg tattgtgttc gtgatcccgt ggatggacga gttttccatc atcggcacca
    58681 ccgatgtgga atacaaaggt gatccgaaag ccgtgaagat tgaagagagt gaaatcaatt
    58741 acctgctgaa agtgtataac acgcacttta aaaagcagtt aagccgggac gatatcgtct
    58801 ggacctactc cggcgtgcgt ccgctatgtg atgatgagtc cgactcgccg caggctatta
    58861 ctcgtgatta cacgcttgat atccatgatg aaaacggtaa agcgccgctg ctgtcggtat
    58921 tcggcggtaa gctgaccacc taccgcaaac tggcggaaca tgcgctggaa aaactgacgc
    58981 cgtattatca gggcattggt ccggcgtgga cgaaagagag cgtgctaccg ggcggagcca
    59041 ttgaaggcga ccgcgacgat tacgccgcgc gtctgcgccg ccgttaccca ttcctgaccg
    59101 aatcgctggc acgtcactac gcgcgcactt acggcagcaa cagcgagctt ctgctcggca
    59161 atgccggggc gataggcgat ctcggggaag atttcggaca tgagttctac gaagcggagc
    59221 tgaaatacct ggtggatcac gaatgggtcc gccgcgccga cgatgccctg tggcgtcgca
    59281 caaaacaagg catgtggctg aatgcggaac aacaatctcg tgtgagtcag tggctggtgg
    59341 agtatacgca gcagaagttg tcgctggctt cgtaaggatg atgcccggtt gtgaagtgca
    59401 accgggctat ttggattctt agactattca ttgtaaataa ttgaataacg gcccctgagc
    59461 taaaatcgcc aaacttaagc acgttaccca ctcaccccac ttctgcctct ggcttctatt
    59521 ttttctgcac caatggcaat aatatcccaa tgcaaaacca agctgtgcac taatcgggcc
    59581 gacaaaatca gtccacggct gcggccaaaa aggggaaaaa ttcaggggag gccagacaaa
    59641 cgtaagaaag aaatttcgga tattttcctg caacgctgat ttctctggag aaatataaag
    59701 aatgaaattc gagagaatta accagaaata gaccggagaa aagaccagtc agcaatgcag
    59761 tctgtttttt aattcaggag gcgttctgcg ggagaattgc caaccgatgc cacccattac
    59821 actgaaaaaa agtgcatatt caaccattaa ctcaagttgt aattcctgaa gagaaatacg
    59881 cacaaccagg agcggaaagg aaaatattat ttctgccaga aaagttaacg tgaatataag
    59941 tacggctaag acaataaccg tcggccatac caataatggc acgataacaa aaccgagaat
    60001 tacaggggga atataaagcc ggaatttaat catttttatt ttcctggtga ttcggatgat
    60061 gcgtcataca tcgcgcataa acactcatca atcatccagt cgtcttccca gacctgataa
    60121 tgtgtgccct gttcgggctg attaagcggg ttctcaatcg cctgccgcgc gcgttctttc
    60181 aggatagatt gattattact agcccaagta ttagtttgag tggtaatggc tttatcgata
    60241 tcgtcctggt tatctctgat ataaccgtca cgatacgtat tcaataaaca ggcaagagac
    60301 gctgccgacc aaaagccggg aactgaaata atggcatcaa gcaatggtga aagagcgata
    60361 tcggcacgga aaaacatttc cagcatgtac tgtaattcga caatttcatc gccggatacc
    60421 caatcagtca tatactggca agcaaattgc tgcaatattg cccgttcatc ctcacgccag
    60481 ttttctttac tggcaagatt tatacgccgt agagaaaata cttcgtgaat attgtcaaat
    60541 tgcccctgtg caacaaactc cagtaaacga ggaagaaaat aacgaacctc atcgctgttt
    60601 tgcggatcag gtcctggact actattccag gcattgatta aggaataagg aatcgctcgc
    60661 aacgatgtat tccgtaatgc tttttgttcc tgttgactca ggcaatattc acaacaaacc
    60721 acaaatttct gcggcaccgg ataacgacga aaaatatgat aaacctgctc aattactgcc
    60781 tgcatagcgg ggctgggttg cataattacc ccctcgcccg ctgatcgtgc ataaacggct
    60841 gatagataaa aagggcattc gccgcgttca ggaaaatatt aatgatatcc atattgtaat
    60901 cctgcttaat gcaacgtttt atacagcggc acatatccca catcttctac cggactctgc
    60961 ccctgcggcg ttaaaaacca ttcgaccagt ttttgtgttt ctgacgttgt attttctctc
    61021 gtcaccataa atgcatcgac gatgtacgca tatttgccgt tgcgaatgtt ttccgccgtc
    61081 ggtgtaatac cgttaatcgc tagcaatttt atatttttat cagcattcat ttgcgtcgcg
    61141 taatagcgga aggtatagcc tattgcgttg tttgtattac ggtattcggc aacgacttta
    61201 atcattccct ccatcacgct tgccacttcc gtttcctgcg gcgagatcat gcggacattt
    61261 ttcatgacct gtgattgcat cactgtctgg ctgccagagt cttccgggcg ctgccaggtc
    61321 tggatctcct gatcgttacc gccaacagta cgccagttgg taattgcacc actgaaaatg
    61381 tcacgcacct gttgttcagt cagggaatta accggattat ccgcattgac gatgaaaacg
    61441 aatgcttcac gggcaaaggg ggtgtacagc aaagtgacac cagattcctt tgcacgtttt
    61501 ttctgcccac cggaaagttg cgccacgaaa ataatatcag catcgccctt aacgattttg
    61561 ttatatgctt ctggcgtacg agagttattc agataatccc aaacgtgaaa atcctctggt
    61621 attatgctta acgcataaaa ggcagaagct taaattgggt acgccgccgt ggcaccatcg
    61681 attcgcggcc agttttgcgt aaactgaatt tgcggtttgc cacgcagcgg cgtcagtcga
    61741 ttattgagtt tatcgggtcg ccaggcccag atatcaatat tttcacgtac accaacacca
    61801 gggttttgct gcttatataa atgggcctga tacccagcga caataccaag taagagaagt
    61861 agaaacagga gtaagttacg gcaacgtagc ccggacgtgc cggttaaagg atgccttgtc
    61921 ggccagccgt atcccagcga aaaactgata tgcgttccaa cgggaataat gactatccac
    61981 cagtactcac caccgaataa tgagactaac aaaacaccca gccagggaag cataagatct
    62041 gccaaccaac ctgaaaccat ctggcgaaat tgataatctg ccagatgtag cgcgattatc
    62101 cagccaacca ggattaataa agcgggaact aaaaaaagac attgtcgcca ggcggctatt
    62161 tctctgtttt ctggtgcgct atcacccgcc cacactcgcc cagagaaaaa tagcagtaat
    62221 gcgcccagcg tcagcataaa gacgttgagt agaagataaa ttgttggcgt aaagagtaaa
    62281 ttgcaaaact caagaatagc ggcaagcccg gcccccagca tggcaataac cgccgccaaa
    62341 aactgcgcca gaatggggat gccgaaaaaa gtcattacca gcgaggtcaa aatccatttt
    62401 ctgttttgca ttattctttc cattctttct gaatggcgaa attatactcc ccgagtcccc
    62461 ttgccccttc tggacacttt tccgaaatga tggcgaaaaa aacgggaccc tttggccccg
    62521 ttctatttat tagtgaaatt acaatctcac cggatcgata tgccagatat gatcagcgta
    62581 ctctttgatc gtacggtcag aagagaagta gcccatattg gcaatgttta gcatcgcttt
    62641 tgcggtccac tcttcctgac gctcgtagag ttcgtcgact ttatcctgac aatcgacata
    62701 gctgcgataa tccgccagta cctggtagtg atcgccgaag ttgatcagcg aatcaaccag
    62761 atcgcgatag cgacccggat cttccggact gaatacaccg ctgccgattt gcgtcagcac
    62821 ctgatgcagc tcctcatctt tctcgtagta ttcacgcggt ttgtagccct gacgacgcag
    62881 ttcttccact tcttccgctg tattaccaaa gataaagata ttgtcagcac cgacatgatc
    62941 cagcatctcg acattcgcac cgtccagcgt accgatggtc agcgcgccgt taagcgcaaa
    63001 cttcatgtta ctggtgccgg aagcttccgt ccccgccagc gaaatctgtt cagacagatc
    63061 ggccgccgga atgatcaact gcgccaggct aacgctgtag ttcgggatga acacgacttt
    63121 cagcttatcg ccaatctgcg gatcgttgtt gatcactttc gctacgtcat tgatcaaatg
    63181 aataatgtgc ttcgccatgt aataggctga agccgcctta ccgccaaaaa tattcacgcg
    63241 cggtacccac ttcgcatccg ggtcggcctt gatgcggtta tagcgggtga tcacgtgcaa
    63301 cacattcatc aattgacgtt tgtattcgtg aatacgttta atttgcacat cgaacagcgc
    63361 tttcggattt accaccacat tcagctgctg ggcgatatac tccgccagac gctttttgtt
    63421 ctccagcttc gcctgatgca cagcgtgatt aaccattggg aaatcacagt gttgttgcag
    63481 ctcattaagc aggctaaggt cggtgcgcca gttacggccc aggtgttcgt ccagcacggc
    63541 tgaaagcgat gggttcgcaa ccgccagcca gcgacgcggc gtcacaccgt tggtgacgtt
    63601 ggtgaaacga cccgggaaga ttttcgcaaa gtcggcaaac aacgattgca ccatcaggtt
    63661 agagtgcagt tccgatacac cgttaacttt gtggctcacc acaaccgcca gccaggccat
    63721 acgcacacga cgaccgttgg atttatcaat gatcgacgcc cgtcccagca gatcggtatc
    63781 gttcggatac tgttcctgca aggttttcag gaaatagtcg ttaatttcaa agatgatctg
    63841 cagatgacgc ggcagaattt tacccagcat atcaaccggc caggtttcca acgcttcgct
    63901 catcagcgtg tggttggtgt aagagaaaac ctcgcaacag accccaaacg cgtcgtccca
    63961 actaaattgg tgctcatcaa tcagcaggcg catcaactca ggaatcgaca gcaccggatg
    64021 ggtatcgttg agatgaatcg cgattttatc cgccaggtta tcgtaggttt tatgcaactg
    64081 ataatggcgg cttaaaatgt cctgaatggt cgaggaaacc aggaagtatt cctgacgcag
    64141 gcgcagctca cgcccggagt aggtggagtc gtctggatac agtacgcgag atacgttctc
    64201 ggagtggttt ttatcttcca ctgccgcgaa gtagtcaccc tggttgaatt taccgaggtt
    64261 aatttcgcta ctggcttgcg cactccacaa acgcagcgtg ttggtcgcgt cagtgtcgta
    64321 acccgggata atctgatcgt aagctacgcc cagaatctct tcggtttcaa tccagcgcgt
    64381 ttttttacct tcctgctgaa tgcgaccgcc aaaacggact ttatagcgcg tgttgtggcg
    64441 tttgaattcc cacgggttac cgtattccag ccagtagtca ggtgactctt tctggctacc
    64501 gttaacgatg ttctgcttga acataccgta gtcatagcgg atgccgtaac cgcgccccgg
    64561 caaccctaac gtcgccagag aatcaaggaa gcaagccgcc agacgtccca ggccaccatt
    64621 accgaggccc gggtcatttt cttcatcaat cagctcttcg agatttaacc ccatcgcttc
    64681 cagtgcgccc tgtacatctt cgtaaattcc tagcgacaac atggcgttgg aaagcgtacg
    64741 gccaatcaaa aactccatcg acaggtagta aacctgacga gtttcttgcg acaactgggc
    64801 acggtttgaa cgtaaccagc gctccacgag acgatcgcgc acagcaaata acgttgcgtt
    64861 cagccattca tgtttattgg cgacgaccgg gtcctttcca atcgtaaaca tcagcttgta
    64921 agcgatagag tgcttaagag cttctacgct aagcgtgggc gatgaatatg taaacggagc
    64981 attcatatag gcgtttcctg aaaactattt caagcgatag taaagctcac ggtacgactt
    65041 cgccgcgacc tgccagctaa aatccattgc catagcctga cgttgcacaa accgccacag
    65101 tgaaggacgg gaccacagta caaaagcacg tcgaatagcc cgtaacagcg accaggcatt
    65161 actatcttca aagacaaacc cactggcgac gccatctgca aggttctcaa gagaacagtc
    65221 agaaaccgta tcagcaagcc caccggtgcg ccgcactaac ggcagcgtac cgtacttcaa
    65281 tccataaagt tgcgttaagc cgcacggttc aaaacggctg ggcaccagaa tgacgtccgc
    65341 gccgcccata atgcgatgcg aaaatgcttc gtgatagcca atctgaacgc ccacctggcc
    65401 ggggtattcc gctgccgccg caaggaaacc ttcctgcagc acaggatcgc ccgcgccgag
    65461 tagcgccagt tgcccgccct gctccagaag acccggtaag gcctccagca ccaggtcgag
    65521 acctttctgg ctggtcagac ggctcaccac tgcaaaaagc ggcactttat cgtcaacctt
    65581 aagccccatt gcgatttgta actggcgctt attttccgct ttatcttcca acgtatcgcg
    65641 ggtgtaacgc gaggccaaca gtaagtccgt ctctggactc cagattttct cgtccacgcc
    65701 gttcagtacg ccggaaagac gcccttcacg gtgacgctgt tgcaacagac cttccatacc
    65761 gtaggcaaac tgcggttcgg tgatctcgcg agcgtaggtt ggactgactg ccgtaatgtg
    65821 atcggcatag tacagaccgg ccttcagaaa agagatttgt ccgttgaatt ccagcccatg
    65881 aatattaaag aatgaccatg gcaattggat gtcattcatg tgatgtgcat aaaacatgcc
    65941 ttgataggcc aggttgtgca cagtaaacac cgacttcgca ggacgcccgc gcgccgccag
    66001 atacgcaggc gcaaggcctg catgccagtc gtgcgcatgc accacatcag gacgccagaa
    66061 tgggtcaagc ccgctagcca tttctgcccc aacccacccc agcagcgcaa aacgcaatac
    66121 gttgtcggta taggcaaata agttggtatc gtgatacggg cttcccggac gatcatagag
    66181 atgcggcgcg tcaatcaggt aaatgccaac cccgttgtaa tgaccgaaca acagcgtgat
    66241 atgtccggcg aaggtatcac gacgggatac tacctgcgca tcggtcacgc cacggcgaat
    66301 atcgggaaat gcaggcaaca gtacgcgagc gtcaacgcca tctgcgattt gtgctgcggg
    66361 taatgcccca ataacatcag ccagaccgcc ggttttaagc agcgggaaca tctctgaaca
    66421 tacatgtaaa acctgcatta tcgctcctgt ttatgcccta acttccgtag catttcgcgc
    66481 gttaccagca cgatgccttc ttctgaacga tagaaacgac gtgcatcttc ctctgcgttt
    66541 tcaccaatca ccatgccttc cggaataaca caagcacgat cgatgacgca gcggcgcaga
    66601 cggcacgagc gacctaccca tacttccggt aacaatacgg cggaatcaat gttgcagaat
    66661 gaattcacgc gaacgcgcga gaacagaacg gactgcacca ccaccgaacc ggagatcaca
    66721 caaccgccgg ataccagtga gttaagggtc atcccgtggc taccggagcg atcctgcacg
    66781 aatttcgctg gcggtaatga ttcattgtag gtgcgaattg gccaattgcg atcgtacata
    66841 tccagttccg gcaccacaga ggccagatcg aggttcgctt tccagtaagc ttccagcgta
    66901 cccacatcgc gccagtacgg ctcggcatcc gggtcggatt gtacgcaaga gagcgggaac
    66961 gggtgcgcat aggccagacc ggcttcggtg atcttgggaa tcaaatcttt gccaaagtcg
    67021 tggctggagt tctcatcgcg atcgtcttct tccagcagtt catacagata gtcggcgtca
    67081 aagacgtaga tacccatact cgccagagat ttgctcggat cgttcggcat tgacggcggg
    67141 ttagctggtt tttcaacgaa ttcgataatt ttatcgttct catcaaccgc cataacgcca
    67201 aatgcggagg cttcttcaat cggtactggc atacaagcaa cggtgcaacg tgcgcctttt
    67261 tcgacgtggt cgataagcat acgcgagtag tcttgcttgt agatatggtc gcccgccagg
    67321 atcaccacgt attccgcttt ataacggcgg ataatgtcga ggttttgggt aactgcatct
    67381 gcggtgccgc gataccagtt ttcccctttc attctctgct gtgctggcag cagatcgaca
    67441 aactcgttca tttcttcatt gaagaatgac cagccgcgct gaatgtgctg caccagagtg
    67501 tgggactggt actgggtgat cacgcccata cgacggatac cggagttgat gcagttagac
    67561 agcgcaaagt cgataatgcg gaacttaccg ccgaagtgta cggccggttt tgctcgctta
    67621 ttggttaaat ccttcaggcg ggtaccacgt cctcccgcca gtatcagggc aacagatttc
    67681 aatggcagct ggcgcgccaa cattaagtga tcgttcttct ctaaactaac catgactaac
    67741 tcctttttta tcatctctgg aacacacaca atccgtgtgc aggtccctgc cagacagccg
    67801 taatcactgg gttatcctct ccagcgaatg ggggaatggc gtgccactcc ccagcaggta
    67861 aaacaatctc tgttacctca agcgtggcgt taattgcgat caaaaagcga tccgagagca
    67921 gaatttgcag ctgtttcggc ccgttttgcc actcatccgt gcttaaaggt tgagcatatc
    67981 gatttagcca acggacattg ccgtcgcctt cttcccacca gcgattctcc accaaagcgg
    68041 gaatgcgctt gcgcagatgg attaacgcgg cggtaaatgc ggttaaacca ctgcttgcct
    68101 gcgaccagtc caaccaggtt aattggttat cctgacagta ggcattgtta ttgccatgct
    68161 ggctgtgacc atgttcgtca ccggccagta acatcggcgt accctgggag agcaacaacg
    68221 ttgttaacag ggcgtgaatg ctgtcgcgac gccgttcaac caggtcaaga gtaccgccta
    68281 acccttcttt accatgattg ttactgtaat tgttgttggt cccgtcgcga ttttcttctc
    68341 cgtttgcttc attgtgttta tggttgaagc aaacgcagtc gcgaagcgta aaaccgtcat
    68401 gcgcggtgac gagattaatc gcggcactcg gcagacgacc attacgttta aaaacatcgc
    68461 tggaggcagc aaaacgcccg gcaaacgccc ccagaggcaa atcataatga agccagaaac
    68521 gacgggcagc atcgcggaaa tgatcgttcc actcggcaaa cagcggcggg aaatttccca
    68581 cctgataacc accaggagca atatcccacg gttcagcaat taacttcacc tgcgagagca
    68641 ccgggcagtt ctggatagcg gtaaacaacg gcgcatcctg acggaactct ggcgtacggc
    68701 ccatgactgc cgccagatca aagcggaaac catcgacgtg gcaggtttct acccaataac
    68761 gcaggcaggc gctggcataa tccaccaccg ccggatgact caaattgagc gtgttgccac
    68821 aaccggtcca gttgtgataa tcgccgtctt ctcttatcca ataatagcta cggttgtcga
    68881 tcccacgcag agagaataac gggccgtcga ggtccagttc cgcactatgg ttgagcacga
    68941 tatcaagaat gacttcgata cccgctttat gcagtgcttt gattgcatcg cgaaactcat
    69001 ccagcgccgt ttctggggag caggcatacg ccggatgcag cgcaaacatc gccaccgggt
    69061 tataacccca gtagttactt agacccatac gttgcaggcg tggttcgctg gcaaactgcg
    69121 ccaccggcag cagttccagc gcggtaatgc ccaactgttt caaatagttg atcatcaccg
    69181 gatgccccag ggctttataa gtgccacgga tctcgaccgg gatctccggg tgcaggtacg
    69241 ttaatccttt gacatgggct tcataaatga tggtactgcc ccacggcgtg cgcggcggag
    69301 catcatcctc ccagtcatag tgatcaacca ccactacgca tttcggcgca atggcggcgt
    69361 tgtcgcgata gtcaggttca ttatgaccgg cgtgcagcag cgggttatct ttaaattccc
    69421 cgtcaatttg ccgcgcgcaa ggatcaatca acaacttcgc cgggttaaag cgatgcccct
    69481 cggcgggttg ccaggggcca tgaacgcgat aaccataacg caaacccgga cgcgcatccg
    69541 gcagataacc gtgccaaatg tcgccactgt gccctggcaa gtcatagcga tgttcctggc
    69601 cattggcgtc aaagatacac agttctaccc gctcggcatg agcggagaaa agtgtgaagt
    69661 tgacgccctg accgtcgtaa tgcgcgccga ggggtgcggg tttgccaatg gcgagttgtg
    69721 tcattctgcc tcccgaacca gccagatagt ggccagcggt ggtagtgtca ggcttagtga
    69781 atgctgacga ccgtggctgg caatctcatc gctgtgtacc gtgccgccat tgccggcatt
    69841 actgccgtga tagtgcatgg aatcggtatt gaggatttca cgccatttgc ccggctggtt
    69901 aatgccgaag cgataatcat gacgcggcac cggagtaaag ttactggcaa cgatgatttc
    69961 gttaccctct ttatcgcgac gcacaaagat cagcaccgag cgttctttgt catccaccac
    70021 cagccattca aagccgtacg ggtcaaaatc cagttcatgc attgctttat ggtggcggta
    70081 ggtgaggttc agatcgcgca ccagacgctg gacaccgtgg tgccagttat cgccgccttc
    70141 caacagatgc cagtcgaggc tggcgtcatg gttccactcg cggccctggg caaattcatt
    70201 ccccatgaac agcaatttct tgcccgggaa tgcccacatc cagccgtagt aggcgcgcag
    70261 gttagcgaat ttctgccatg cgtcgcccgg catccggtcg agaatcgatt ttttaccgtg
    70321 gaccacttca tcatgcgaca acggcagaac gaagttttca gtgtagttgt agagcatccc
    70381 gaaggtcagt ttatcgtgat gatactgacg ataaaccggg tcgagcttca tgtagtccag
    70441 ggtgtcatgc atccagccta ggttccactt gtaccagaag cccagaccgc ccatgtcctg
    70501 cggacgagaa acgccaggga aatcggtaga ctcctccgcc attgtcaccg caccggaaac
    70561 ctgctcacca agaatacggt tggtattacg caagaattca atcgcttcaa gattctcgcg
    70621 cccgccaaat tcgttcggga tccactcccc ctctttacgg ctgtagtcac gataaatcat
    70681 tgacgccacc gcatcgacgc gcagcgcatc aataccaaaa cgttcgatcc agtaaagcgc
    70741 gttaccgaca aggaagttac tgacttcacg gcgaccatag ttgtagatca gcgtattcca
    70801 gtcctgatga tagccttcgc gcggatcgct gtgttcatac aagttcgtgc catcaaattc
    70861 ggcaagcgca aagtcatcgg tcgggaagtg gcctggcacc cagtcgagaa tcacgttcag
    70921 accagctgcg tgtgcggcat caatgaaata acggaagtcg tcgcgggtac caaaacggcg
    70981 ggtcggtgca tacaggccgg ttggctgata accccaactg ccatcgaacg gatgctcgtt
    71041 aatgggcagc agttcgaggt gggtaaagcc catccattta gcataaggca ccagttgatc
    71101 ggccagctcg cggtagctta accagaaatt gttgtcggtg tggcgacgcc aggagcccag
    71161 gtgaacttca taaatagaga ttggcgcatc aaactgattc gcttttttgc gctcttcagt
    71221 ctgcacaacc ttttccggta gcccgcaaat aagagacgcg gtttccgggc gcatttgcgc
    71281 ttcgaaggca taagggtcgg acttcagacg caagttgcca ttggcatcaa tcatctcgta
    71341 tttatagagc tgaccgttat gcgccccagg gataaacagt tcccagatgc cgctctcttt
    71401 acgcagacgc atcgggtgac ggcgaccgtc ccagtagttg aattgcccaa ccaccgagac
    71461 ccgacgggcg tttggagccc agacagagaa acgcgtacct gtgacgccat ccatagtatc
    71521 tgcatgcgcg cctaaggttt catacgggcg caggtgagta ccttcagata atagccaggc
    71581 atccatttcc tggattagcg gaccaaaacg gtaaggatca tctatcaggt tttgctgacc
    71641 atgccagaca acagccaact gatagcggaa aaaattctta cgtcgcggaa tgacaccgct
    71701 aaagaatcca cgggagtcga gacactccag ttttgcgagt ttgcgcccgg ttttcggttc
    71761 aatcacccac acatcggtag cgtcgggtaa aagggcacgg acttccagtc ccgcggtggt
    71821 tttatgcatc cccagtacgg aaaaaggatc cgcaaaatgg cctgcaatta gcgcgttaat
    71881 cacgtctcta tcgatacgat cggacatgct tgtcttcctg ttttattgtg tcaccccatc
    71941 caactggatt tttgacttct ggttgtgaca tttttttgac ctgaacggcg cagcactctg
    72001 tgcatcctct ctgcgtcgtc ctcacttcag gtaaggctgt gaatactcat gtattcagcc
    72061 acccttaaag aatagccaat gctctattta actcccggta aatcatgaaa catctgcgct
    72121 tactcctgta ttacgcacta acaggggcgg catcgcgccc cagatttaat gaataaagat
    72181 tacgccagtt gacgaagcat ccgacgcagc ggctccgcgg ccccccacag cagctggtcg
    72241 cccacggtaa aggctgacag gaactctggt cccatattca gcttacgcag gcggcctacc
    72301 ggcgtggtca gcgtgccggt aacggcagct ggggttagct cacgcatagt gatttcccga
    72361 tcgttcggaa cgactttcgc ccacggattg tgtgcagcca gcagttcttc cacggtcgga
    72421 atagacacat cttttttcaa tttaatagtg aatgcctggc tgtggcagcg caatgccccg
    72481 acacgcacac ataaaccatc taccggaatt acggaagatg tgttgaggat cttgttggtt
    72541 tccgcctgcc ctttccactc ttcgcggctc tgaccgttat cgagctgttt gtcgatccac
    72601 ggaatcaggc tacccgccag cggcacgcca aagttatcca ccggcagctc accgctacgg
    72661 gttaaggttg tgactttgcg ttcgatatcg agaatagcag aggacggggt cgcgagttca
    72721 tctgccacat ggccatacag atggcccatc tgggttaata actcacgcat atgtcgcgca
    72781 ccaccgccgg aagcggcctg gtaggttgca acggacaccc aatcaacaag atcattggcg
    72841 aataaaccac ccaacgacat caacatcagg cttacggtac agttgccgcc aacaaaagtc
    72901 ctgatgccat tatttaatcc gtcggtaatg acgtcctgat tgacggggtc aagaatgatg
    72961 atggcgtcat ctttcatgcg cagagatgat gctgcgtcaa tccagtaacc ttgccatccg
    73021 ctttcacgaa gctttggata gatttcgttg gtataatcgc cgccctgaca ggtcacaatg
    73081 atatcgaggg cctttagcgc ctccagatca aaggcatcct gaagtgtgcc agtggttccg
    73141 ccaaaagacg gcgcagcctg accaagctga gaagtagaaa agaagacagg gcgaatggcg
    73201 tcgaagtcgc gctcttcaac catgcgttgc atgagaacgg agccgaccat accgcgccag
    73261 ccgataaaac caacattttt cataagcgtt ttttcctgca aagatgtgtg ctgtataaat
    73321 gtgccggtct cctcttggca catctttcac catacaaaaa gcagccaaag tcgcaagtga
    73381 aattaatcaa tgatagcgaa gccatcagta atgcgactta tcctgctttg ttagcacgca
    73441 gaaagtccgc ggcaattatc agggaatttg agttatgaat gaaatcattt ctgcagcagt
    73501 tttattgatc ctgattatgg atccgctcgg aaacctacct attttcatgt ccgtactgaa
    73561 acatactgaa ccgaaaagac ggcgggcaat catggtgcga gagttgctta ttgctctcct
    73621 ggtgatgctg gtgttcctgt ttgcgggtga gaaaattctg gcatttctta gcctgcgggc
    73681 agaaaccgtc tccatttctg gcggcatcat tctgtttctg atcgccatta agatgatttt
    73741 ccccagtgct tcaggaaata gcagcgggct tccggcaggt gaagagccgt ttatcgtgcc
    73801 gttggcaatt ccgttagtcg ccgggccgac tattctcgcc acgctgatgt tgttgtctca
    73861 tcagtacccg aatcagatgg gacatctggt aattgctctg ctactggcct ggggcggcac
    73921 ctttgtcatc ctgctacagt cttcgctatt tttacgtctg ctgggcgaga aaggggtgaa
    73981 cgcacttgaa cgcctgatgg gattgattct ggtgatgatg gcaactcaga tgttcctcga
    74041 cggcattcga atgtggatga aggggtaacg aggagagttt ttcgaaaaga gcccggtagc
    74101 gtcacaacta ccgggcgaac aaacttaact caacagctgg aaagcaatca tcccaacgat
    74161 ggcaccaaca gtgccgagga tggtttccat catggtccag gttttcagcg tttcggcttc
    74221 ggtcgcgccg gtaaatttac cgaacaacca gaaaccggcg tcgttaacgt ggctgacaac
    74281 aatcgaacca ccggcgatac aaatcgacag cgccgccatt tgcgcaccgg agtagttcag
    74341 ttgttcaata accggcatca ccagtcccac cgccgttaaa caggcaacgg tggcagaacc
    74401 ctgaatgata cgcaccgccg cagccagcac aaagcaggtg atggcaatcg gcaggcccat
    74461 gccggttaac gcttcgccca gtgccggacc tacgccagag tcaaccagca cctgtttgaa
    74521 cacgccgcct gcaccaatca ccagcagaat gatccccgcc ggttgcagcg cgtgaccgca
    74581 aatctccatc actttgtctt ttggcatgcc ctgacgcatt gccaggccat aaatcgctac
    74641 cagacaagca accagaatcg cggtaaacgg atgaccaata aactcgaacc attcgtaagc
    74701 ggtagagcct tctggcacaa aacgcgcggc aatggttttc agccctacca gcaccagcgg
    74761 caacaggatc agcgacaggc tgaatccgaa agatggcatt ttgccttcgc cgagatgcgg
    74821 ttcgctgatg tcgtcaggaa tatgcaactc cacgtagcgg ctgatgaaat taccccacag
    74881 cggcccggca ataatcattc ccggaattgc cgcacacagg ccaatcagga tcatccagcc
    74941 aaagtcggcg ttcatctgcg atgccagcag cattggcgct ggccccggta ccaggaacgc
    75001 agccgcggcc gccacgcctg cgaataatgg gattaccagt ttcaccaggt tcgtaccggt
    75061 gtggcgcgcc attgagaaag caacgctaat cagcagaaca atcgccactt caaagaacag
    75121 cggtagcgca caaaccagcc ccgcaaggcc gatggcataa tgtgctcggc tgtgaccgaa
    75181 ggatttgagc attttgacgg caatctgatc gactgcgcca gtttcatgta agatcttgcc
    75241 aaacatagct cccagggcga caaccaccgc caggaagccg agggtgcctc ccatcccttt
    75301 ttccatcgtc gctgcgattt tatcgagcgg cataccggaa aaaaggccag cccccatgga
    75361 caccaccatc aaagccagga aagcgtgcat acgcgccttc atgactaaaa acagcagcag
    75421 taaaacagac cctactgctg ttaaaacaag cgttaatgta gtcactactt atttgccttt
    75481 tttaataacc tcaatggtgc ttgccacaac accttccagc ggttgatcga tatccaccac
    75541 cagtacatcg gtttcgtccg cacccggctc ctgcagcgtt tcaaactgcg tcaccaacat
    75601 ttgggtttta aagaaatggc ctttgcgcgc tttcaggcgg ctttcaatca catcaaaatc
    75661 gcctttcagg tagatgaacg agagattcgg attaccttca cgcagcaagt cgcgatagtg
    75721 ttttttcagt gcagaacaga cgatcagcga cactttattg gtgcgctgca tagcaaacgc
    75781 ggcgtcgttc agcgcctgca accacggttt gcgatcgtcg tcattcagcg gttcaccaga
    75841 cgccattttt tcgatattgc gccttggatg gaggaaatcg ccatcaagaa acgcggcatg
    75901 aagttgatgc gccacttcac tggcgaccgc agatttgccg ctgcccgata cgcccatcaa
    75961 gacgtaaatg tggtgatcat ggttagtcgt gctcaaagtg gtgcccccac aatacaagaa
    76021 ataaaaatgt tacgggtaac tgttatcggt aacattgtcc ggccggacaa tatcagaagc
    76081 aatatccatc cgtgccttaa gtgtataagt gtgagctact tcaaatttgt gggcttaaat
    76141 agatccgccc ggtgacaagg tgaaacctaa atctaacatt ttcggtgtca cagattcgcc
    76201 acgaatacgc gccagcaggc gttcagcgcc aatactgccc atccgctcac gcggcgtcag
    76261 tacgctcgcc aaccgtggtt ccatcacctg accaatgtca tgaccatgga aaccggcaat
    76321 cgccatatcg tcaggaactt tcaaccccag acgctggcat tcaaacgccg cgccgaccgc
    76381 caggtcatca ttagtacaga acacgccatc cagctgcgga tattcccgcc tcgcctggcg
    76441 aatcagttca ataccggaag agtaagaaga agattgctca accatcacgc tatacggcac
    76501 caggcctgca tccagcatcg cctgttcgta tcccttctgt ttgatgatag tacgttcgtc
    76561 gagacgtgcg ccgagatagg caatgtggcg atgcccgcga gcaataatgg cggtggtcat
    76621 ctggcgtgcc gcttcaaagt tatcaaaacc gacggcaata tcgaggcacg gcgacttgct
    76681 gtccatcaac tccaccaccg gaatccccgc cacttcaatc atctttaagg tgcgcggcgt
    76741 gtgggtacgt tcggtgagga tcagtccgtc gatattccat gagagcattg attcgaggcg
    76801 ttcttgctcc atttccggtt tataaccgta gtgggccagc atggtctgat aaccgtgcgc
    76861 gtcggtgacg ctttcgattc cgcgtaatac ttccgcgaaa acctggttgg tgagagaagg
    76921 taacaggacg ccaatcgccc ggctggtggc gttagagagg atatcgggcg cgcgattggg
    76981 aatataaccc agttcatcaa gagcggcggc aatcttgccg cgtagagcga cggaaacctg
    77041 ctccgggtta cgcaaaaagc ggctgaccgt cattttggtc acgcctacac ggtcagccac
    77101 atcctgaagt acgggtcttt tctttttcat cgtcctgaag gtacaaaaga gatagattct
    77161 cttagtttaa cacggacgca tcagaacctt cccggggaaa acagtgcttg tttacaatta
    77221 ttagatatac actaaatcaa ttgcatcgca ttgtgctaac gacgcggcaa aaccccggaa
    77281 gcgtacatca gcacgcgacc ggagcgagcg aggaagcctg caaaaaggca ggcttcaaag
    77341 atgccgttga gttttaaacc ggcggcagat cgaacagtaa cacttcgctg tcgctatccg
    77401 catgaatgga gattgcctgc tcatcccaga ttgccagacc atcactggtc gaggctttca
    77461 cgccgttaat ggtgacattg cctttcacca cctggatcca gacgcggcgt tcagcggcaa
    77521 tctgatgcac cgactgctca tctttcagca atgcccaacg atacagttcc atatcctgat
    77581 gcactttcag cgaaccatct cgcgcatccg gcgagagcac cagctgtttg ccctgtacgg
    77641 catcgaagcg acgctgttca taacgcggcg taataccgtt ttcttccggc atgatccaga
    77701 tttgatacag atgcagacgc tcggtgctgc ttgggttgta ctctgagtga cgaatacccg
    77761 tacccgcact cataatctgg aactcacccg ccggaacctg ctctttattg cccatgctgt
    77821 cctgatgctc aacagtacct tccagcacgt aggtcaaaat ttccatatct ttatgcgggt
    77881 gagtgccgaa gccctgccct gcttcaatca cgtcgtcgtt aatcacgcgc agcgcggaga
    77941 agcccataaa gttcggatcg taatagttgg caaaagagaa agtatgccag gagtccagcc
    78001 agccatgatt tgcatgacca cgttcatttg ctttgcgtaa gtagatcatt tttattcgcc
    78061 ccctgaatga tttcgatggg cttagtgtgg acccgatccg cctgggatga tagagggtga
    78121 aaattgaccc ctctgttcaa aaaaattgaa ctattcgagg ggccatcaaa cttacttcgc
    78181 gagagtcact gtggagggag aggcttgctc aaatcctcgt tcaaggattt ccagattggt
    78241 aagaacttca gattccttga cgtaatttgg cgcaccgttg gtgatggttt gatacaacgc
    78301 atcataaacg cgcccgtaat cgcccatctc cggcttcatc tcttctctga ccgtcacacc
    78361 ctcgtcattg acatactcca gcacaccgac cgaatcatcc gctgcgaatc ccggttcgcc
    78421 cggcataata ttagccttga ggctggtttc ctgctggtcg ataccgtatt taataaacga
    78481 acctttctta ccgtgaacga taaatttcgg ataatcgatt ttcaccagat ggctggtttt
    78541 gacgatggct ttcaggtcgc cataaaacag ctgcgcttca aaggtgtcgt caggattggc
    78601 tttattacgc aggctgcgga tgtcataagc gacgtgatcc gggcgaccga acagagaaat
    78661 aatctggtcc atcgtatgca caccaagacc atagaacgcg ccatcctgcg gcagcccagg
    78721 tttggtttct gccaccgggc ggtaatagtc aaaatggctt tccacttcga caatctctcc
    78781 caacttgcca ctttcaatcg cttttttcgc tgtcaggaag caggaatcaa aacgacgatt
    78841 ctgatacggc gtgacggtca gtcctttgct tttcgccagc gcaaacagct ccttcgcctg
    78901 ggcaagcgtc ggggtgaacg gtttttcgac cagcacattt ttcccggctt ccagcgcgcg
    78961 cttcgcgtac tcgaagtggc tgtctgcatg ggtgcagacc acaaccagct taacatcggg
    79021 atcgtttagt acttcgtcga gatcgctggt gaaatggata tgagagtaaa tgggggcctg
    79081 ttcttccggc ttcgcatggc ggcgaaaaat atgcgcgaca tgccagctat ccttgcggtt
    79141 aagtacatac ggcagatggt aacgggtggt gcttttgccg aagccaataa aggcgcagtt
    79201 gatgaccatg atttcgtcct ttttaaggtg attatttaca ccttagcgca aagcggacgt
    79261 ggttcctact ggagtgcgca taacgaacac aagcacttcc cgtggataaa ttgaaaacga
    79321 a
//
LOCUS       DAPOZO010000006        74002 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPOZO010000006 DAPOZO010000000
VERSION     DAPOZO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.006"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..317
                     /locus_tag="ROI21_000441"
     CDS             <1..317
                     /locus_tag="ROI21_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5109415.1"
                     /translation="ILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAK
                     ENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEIIQA
                     TA"
     gene            361..468
                     /locus_tag="ROI21_000442"
                     /pseudo
     CDS             361..468
                     /locus_tag="ROI21_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(452..1090)
                     /gene="ytfB"
                     /locus_tag="ROI21_000443"
     CDS             complement(452..1090)
                     /gene="ytfB"
                     /locus_tag="ROI21_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDX5109416.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1308..1928
                     /gene="fklB"
                     /locus_tag="ROI21_000444"
     CDS             1308..1928
                     /gene="fklB"
                     /locus_tag="ROI21_000444"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDX5109417.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2237..3649
                     /gene="cycA"
                     /locus_tag="ROI21_000445"
                     /gene_synonym="dagA"
     CDS             2237..3649
                     /gene="cycA"
                     /locus_tag="ROI21_000445"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDX5109418.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3694..4356)
                     /gene="ytfE"
                     /locus_tag="ROI21_000446"
     CDS             complement(3694..4356)
                     /gene="ytfE"
                     /locus_tag="ROI21_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDX5109419.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQASKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGRQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(4464..5429)
                     /locus_tag="ROI21_000447"
     CDS             complement(4464..5429)
                     /locus_tag="ROI21_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDX5109420.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVIIAVRAKPEKPLTESVSES"
     gene            complement(5538..6398)
                     /gene="qorB"
                     /locus_tag="ROI21_000448"
     CDS             complement(5538..6398)
                     /gene="qorB"
                     /locus_tag="ROI21_000448"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDX5109421.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6487..6867
                     /locus_tag="ROI21_000449"
     CDS             6487..6867
                     /locus_tag="ROI21_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDX5109422.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6984..8927)
                     /gene="cpdB"
                     /locus_tag="ROI21_000450"
     CDS             complement(6984..8927)
                     /gene="cpdB"
                     /locus_tag="ROI21_000450"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDX5109423.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNIYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMVNANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            9117..9857
                     /gene="cysQ"
                     /locus_tag="ROI21_000451"
     CDS             9117..9857
                     /gene="cysQ"
                     /locus_tag="ROI21_000451"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDX5109424.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSVAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            10069..10980
                     /locus_tag="ROI21_000452"
     CDS             10069..10980
                     /locus_tag="ROI21_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109425.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFNKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNETVNAMDKFLISFSLKDNGTEYIMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPERIKGYIRQ
                     AFGDVSLGYAVLDDIVSKLKRGKFDIQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     DITEEKICEKYKSTIEAFKKKQLALPV"
     gene            complement(11069..11623)
                     /locus_tag="ROI21_000453"
     CDS             complement(11069..11623)
                     /locus_tag="ROI21_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDX5109426.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            11948..12154
                     /locus_tag="ROI21_000454"
     CDS             11948..12154
                     /locus_tag="ROI21_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDX5109427.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(12216..13559)
                     /locus_tag="ROI21_000455"
     CDS             complement(12216..13559)
                     /locus_tag="ROI21_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDX5109428.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(13882..14520)
                     /gene="msrA"
                     /locus_tag="ROI21_000456"
     CDS             complement(13882..14520)
                     /gene="msrA"
                     /locus_tag="ROI21_000456"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDX5109429.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            14726..16459
                     /gene="tamA"
                     /locus_tag="ROI21_000457"
     CDS             14726..16459
                     /gene="tamA"
                     /locus_tag="ROI21_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDX5109430.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            16456..20235
                     /gene="tamB"
                     /locus_tag="ROI21_000458"
     CDS             16456..20235
                     /gene="tamB"
                     /locus_tag="ROI21_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDX5109431.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVSI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            20238..20579
                     /locus_tag="ROI21_000459"
     CDS             20238..20579
                     /locus_tag="ROI21_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDX5109432.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            20791..21042
                     /gene="chpS"
                     /locus_tag="ROI21_000460"
     CDS             20791..21042
                     /gene="chpS"
                     /locus_tag="ROI21_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDX5109433.1"
                     /translation="MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGDEIW"
     gene            21036..21386
                     /gene="chpB"
                     /locus_tag="ROI21_000461"
     CDS             21036..21386
                     /gene="chpB"
                     /locus_tag="ROI21_000461"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDX5109434.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAAGE
                     VVEEALLRLQAVVE"
     gene            complement(21465..21995)
                     /gene="ppa"
                     /locus_tag="ROI21_000462"
     CDS             complement(21465..21995)
                     /gene="ppa"
                     /locus_tag="ROI21_000462"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDX5109435.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            22305..23261
                     /gene="ytfQ"
                     /locus_tag="ROI21_000463"
     CDS             22305..23261
                     /gene="ytfQ"
                     /locus_tag="ROI21_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDX5109436.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKQCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            23394..24896
                     /locus_tag="ROI21_000464"
     CDS             23394..24896
                     /locus_tag="ROI21_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDX5109437.1"
                     /translation="MNTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKSVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAQRFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            24910..25932
                     /locus_tag="ROI21_000465"
     CDS             24910..25932
                     /locus_tag="ROI21_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5109438.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            25919..26914
                     /gene="yjfF"
                     /locus_tag="ROI21_000466"
     CDS             25919..26914
                     /gene="yjfF"
                     /locus_tag="ROI21_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDX5109439.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(26947..27945)
                     /gene="fbp"
                     /locus_tag="ROI21_000467"
     CDS             complement(26947..27945)
                     /gene="fbp"
                     /locus_tag="ROI21_000467"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDX5109440.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            28121..29494
                     /gene="mpl"
                     /locus_tag="ROI21_000468"
     CDS             28121..29494
                     /gene="mpl"
                     /locus_tag="ROI21_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDX5109441.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDALADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(29646..30197)
                     /locus_tag="ROI21_000469"
     CDS             complement(29646..30197)
                     /locus_tag="ROI21_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDX5109442.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            30291..31643
                     /gene="pmbA"
                     /locus_tag="ROI21_000470"
     CDS             30291..31643
                     /gene="pmbA"
                     /locus_tag="ROI21_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDX5109443.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            31827..32213
                     /gene="cybC"
                     /locus_tag="ROI21_000471"
     CDS             31827..32213
                     /gene="cybC"
                     /locus_tag="ROI21_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDX5109444.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            32405..32647
                     /locus_tag="ROI21_000472"
     CDS             32405..32647
                     /locus_tag="ROI21_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDX5109445.1"
                     /translation="MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRM
                     PVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL"
     gene            32637..32927
                     /locus_tag="ROI21_000473"
     CDS             32637..32927
                     /locus_tag="ROI21_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDX5109446.1"
                     /translation="MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKL
                     RDLPDCYKIKLRTQGYRLVYQVNDKELLVLVIAIGKRENSAVYEDATKRLDE"
     gene            complement(32928..33392)
                     /gene="nrdG"
                     /locus_tag="ROI21_000474"
     CDS             complement(32928..33392)
                     /gene="nrdG"
                     /locus_tag="ROI21_000474"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDX5109447.1"
                     /translation="MNFHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(33577..35715)
                     /gene="nrdD"
                     /locus_tag="ROI21_000475"
     CDS             complement(33577..35715)
                     /gene="nrdD"
                     /locus_tag="ROI21_000475"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDX5109448.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(36109..37764)
                     /gene="treC"
                     /locus_tag="ROI21_000476"
     CDS             complement(36109..37764)
                     /gene="treC"
                     /locus_tag="ROI21_000476"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDX5109449.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKASPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(37814..39235)
                     /gene="treB"
                     /locus_tag="ROI21_000477"
     CDS             complement(37814..39235)
                     /gene="treB"
                     /locus_tag="ROI21_000477"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDX5109450.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNSVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(39354..40301)
                     /gene="treR"
                     /locus_tag="ROI21_000478"
     CDS             complement(39354..40301)
                     /gene="treR"
                     /locus_tag="ROI21_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDX5109451.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            40486..40539
                     /gene="mgtL"
                     /locus_tag="ROI21_000479"
     CDS             40486..40539
                     /gene="mgtL"
                     /locus_tag="ROI21_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDX5109452.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            40680..43376
                     /gene="mgtA"
                     /locus_tag="ROI21_000480"
     CDS             40680..43376
                     /gene="mgtA"
                     /locus_tag="ROI21_000480"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDX5109453.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWSLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(43582..43968)
                     /gene="ridA"
                     /locus_tag="ROI21_000481"
     CDS             complement(43582..43968)
                     /gene="ridA"
                     /locus_tag="ROI21_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDX5109454.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(44041..44502)
                     /gene="pyrI"
                     /locus_tag="ROI21_000482"
     CDS             complement(44041..44502)
                     /gene="pyrI"
                     /locus_tag="ROI21_000482"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDX5109455.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(44515..45450)
                     /gene="pyrB"
                     /locus_tag="ROI21_000483"
     CDS             complement(44515..45450)
                     /gene="pyrB"
                     /locus_tag="ROI21_000483"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDX5109456.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(45443..45589)
                     /gene="pyrL"
                     /locus_tag="ROI21_000484"
     CDS             complement(45443..45589)
                     /gene="pyrL"
                     /locus_tag="ROI21_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDX5109457.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFLPRRQE
                     IKDG"
     gene            complement(45870..46265)
                     /locus_tag="ROI21_000485"
     CDS             complement(45870..46265)
                     /locus_tag="ROI21_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDX5109458.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(46396..47109)
                     /gene="bdcA"
                     /locus_tag="ROI21_000486"
     CDS             complement(46396..47109)
                     /gene="bdcA"
                     /locus_tag="ROI21_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX5109459.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            47180..47773
                     /locus_tag="ROI21_000487"
     CDS             47180..47773
                     /locus_tag="ROI21_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDX5109460.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            47918..48370
                     /gene="tabA"
                     /locus_tag="ROI21_000488"
     CDS             47918..48370
                     /gene="tabA"
                     /locus_tag="ROI21_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDX5109461.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTPETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLRGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            48493..50088
                     /locus_tag="ROI21_000489"
     CDS             48493..50088
                     /locus_tag="ROI21_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDX5109462.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSNRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSTITTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKVLACYQREANKEVALLLNNHQLNWKDAIDYNELLEKPLASTLEKIKKEH
                     LLLMPHVCDDTIPYLLGEGGILEEINGLKTLHDTKIDSGTEGNDEINNIKINLSNILI
                     DSLDDAKINLEKVIDSMLETFFKLPYINDVKILEWCFNQSMQYSDDTTKIKHARSVID
                     HIDFNCDQSKIAETLFFNLNKEPYKNSPELQELIWEKLVVYVNDFNLSNQEKSRLILR
                     LFDDVKLVFDKVPLSILVNDIFLKDFFMKQPDFAKWYFYQLLKSYEGEQMYLNELGYV
                     YGDEEKTKEIVNKLPGYVVKIFEEKIDNELKIRTRMMEILRDEQINIYEYINEKQLEK
                     LNPQGNLRIAIEKFGWNNKPITA"
     gene            complement(50144..51148)
                     /gene="argF"
                     /locus_tag="ROI21_000490"
     CDS             complement(50144..51148)
                     /gene="argF"
                     /locus_tag="ROI21_000490"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDX5109463.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            51310..51726
                     /gene="rraB"
                     /locus_tag="ROI21_000491"
     CDS             51310..51726
                     /gene="rraB"
                     /locus_tag="ROI21_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDX5109464.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGGRH"
     gene            complement(51772..52275)
                     /locus_tag="ROI21_000492"
     CDS             complement(51772..52275)
                     /locus_tag="ROI21_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDX5109465.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEFDGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            52468..53664
                     /locus_tag="ROI21_000493"
     CDS             52468..53664
                     /locus_tag="ROI21_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HDX5109466.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKRFWWVTFFLPILMAIGMGAV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            complement(53720..56575)
                     /gene="valS"
                     /locus_tag="ROI21_000494"
     CDS             complement(53720..56575)
                     /gene="valS"
                     /locus_tag="ROI21_000494"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDX5109467.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADNKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(56575..57018)
                     /gene="holC"
                     /locus_tag="ROI21_000495"
     CDS             complement(56575..57018)
                     /gene="holC"
                     /locus_tag="ROI21_000495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDX5109468.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(57372..58883)
                     /gene="pepA"
                     /locus_tag="ROI21_000496"
     CDS             complement(57372..58883)
                     /gene="pepA"
                     /locus_tag="ROI21_000496"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDX5109469.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            59150..60250
                     /gene="lptF"
                     /locus_tag="ROI21_000497"
     CDS             59150..60250
                     /gene="lptF"
                     /locus_tag="ROI21_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDX5109470.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            60250..61332
                     /gene="lptG"
                     /locus_tag="ROI21_000498"
     CDS             60250..61332
                     /gene="lptG"
                     /locus_tag="ROI21_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDX5109471.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(61493..62995)
                     /locus_tag="ROI21_000499"
     CDS             complement(61493..62995)
                     /locus_tag="ROI21_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDX5109472.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAQAGTVSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDETHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(63073..64071)
                     /gene="idnR"
                     /locus_tag="ROI21_000500"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(63073..64071)
                     /gene="idnR"
                     /locus_tag="ROI21_000500"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HDX5109473.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(64138..65457)
                     /gene="idnT"
                     /locus_tag="ROI21_000501"
     CDS             complement(64138..65457)
                     /gene="idnT"
                     /locus_tag="ROI21_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HDX5109474.1"
                     /translation="MPLIIIAAGVALLLILMIVFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(65520..66284)
                     /gene="idnO"
                     /locus_tag="ROI21_000502"
     CDS             complement(65520..66284)
                     /gene="idnO"
                     /locus_tag="ROI21_000502"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDX5109475.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(66308..67339)
                     /gene="idnD"
                     /locus_tag="ROI21_000503"
     CDS             complement(66308..67339)
                     /gene="idnD"
                     /locus_tag="ROI21_000503"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HDX5109476.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            67556..68119
                     /gene="idnK"
                     /locus_tag="ROI21_000504"
     CDS             67556..68119
                     /gene="idnK"
                     /locus_tag="ROI21_000504"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDX5109477.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(68123..69142)
                     /gene="ahr"
                     /locus_tag="ROI21_000505"
     CDS             complement(68123..69142)
                     /gene="ahr"
                     /locus_tag="ROI21_000505"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDX5109478.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADY"
     gene            69338..69422
                     /locus_tag="ROI21_000506"
     tRNA            69338..69422
                     /locus_tag="ROI21_000506"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69372..69374,aa:Leu,seq:caa)
     gene            69608..70873
                     /locus_tag="ROI21_000507"
                     /pseudo
     CDS             69608..70873
                     /locus_tag="ROI21_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(71190..72370)
                     /locus_tag="ROI21_000508"
                     /pseudo
     CDS             complement(71190..72370)
                     /locus_tag="ROI21_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            72957..73186
                     /locus_tag="ROI21_000509"
                     /pseudo
     CDS             72957..73186
                     /locus_tag="ROI21_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(73700..>74002)
                     /locus_tag="ROI21_000510"
     CDS             complement(73700..>74002)
                     /locus_tag="ROI21_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDX5109479.1"
                     /translation="LWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT        18056 a        18330 c        19179 g        18437 t
ORIGIN      
        1 cgatactgga tcagggctgg tatgaaatgc gccgccagct tgagtacaag cagctctgga
       61 gtggcggtca ggtgcttgct gttccgccag cgtacacaag ccagcgttgc gcgtgctgtg
      121 gtcatacagc gaaagaaaat cgcctgtcac aaagtaaatt cagatgccag gtatgtggat
      181 atacagcgaa cgccgatgta aacggcgctc gtaacatttt agcggcggga cacgccgttc
      241 ttgcctgtgg agagatggtg cagtcaggcc gctcgttgaa gcaggaaccc accgaaataa
      301 ttcaggcgac agcctgaacg tagcagggaa ccacgtcctt cagggcgtgg aggatgtcaa
      361 cacatcagta tggtttcacc ccaggcactg gaagccagac gtaaggtcat caaagagaca
      421 atccttagct ggcttttggt tgacccttca tctaccgcgc acgaataaaa ctgccatccg
      481 gctggcgggt aaacaagacc tgttgattat tccccgtatc aatggttaag cccgtcacca
      541 cgccgctggc gttctggcgg attttcacca tctgcccgtt ttgcaaatta ctcagcggct
      601 tacccgcgcc ttcaacctgc gccattgcat agacatcggt agcgggcagt ccgtggtcgc
      661 ggaacagctg cgccatcgtt ttaccgggtt cgacacggta agagcgccat tgattatcaa
      721 ttccgctatc tggctggaat ggttgcgttt gggtggtttg cggctgctct tctggctggc
      781 cttcctgtat tggttctgga gcgaccgggg ccacctgatc aggatcattt tgtggcgtaa
      841 ctagctgcgc ccgcagctgt tcttctgtcg gtggttgtga ttgcgactga atatctaact
      901 gtgcttcacg cgtcaccacc ggcgcgttgg gcgtatcatc agatggaagc agaaatccga
      961 ccaccaatac aatggcggca ataatgatcc cccgacgatg catcggcggc agcgggtcca
     1021 tgaagcgaaa attgtccggc gcgtgccaga ctttctccag ggttggtttt agttcaaagc
     1081 gcccgggcat ggtttcctcc tgctccgcgt cttgttcctc aatcatagcc tatgaataag
     1141 ctaacgctat gatgtccgtg gtaaacccgc ctttattata ttcatacggg atattgctat
     1201 tgtttctttt tccccgggat ttgtcatcat tcccgcgaca aagttttacc caaagaagtg
     1261 tggctgatat gctgcccgct actttatacc ctaagaaagg aaatacgatg accaccccaa
     1321 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1381 taagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggc attgctgatg
     1441 cgctggaagg caaacatccg gctgttccgg ttgatgtggt gcaccgcgcg ctgcgtgaaa
     1501 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1561 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtaaa tagcaccgaa tctggcctgc
     1621 aattccgcgt gatcaaccag ggcgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1681 ttcattacac cggaaaactg atcgacggca ccgtgtttga cagctccgtt gctcgcggcg
     1741 agccagctga attcccggtt aacggcgtga tccctggctg gattgaagca ctgactctga
     1801 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1861 gcgctggcgc gtccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1921 tcctctaagc agcgcattct gttcccctcg agccagaggg gagcaggcat tcagcaataa
     1981 acccttcagt ttgccaaacg gcgctatttt atgttgcaaa gaccctgtaa gcgtgtattt
     2041 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ttttaaacat aaaattaaca
     2101 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     2161 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     2221 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     2281 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     2341 tgccattggt acagggttgt ttatggggtc cggcaaaacg attagccttg ccgggccgtc
     2401 gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc gggcaatggg
     2461 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2521 cgggccgtgg gcaggatatt tcactggctg gacttactgg ttctgctggg ttgtaaccgg
     2581 tatggcagac gtggtggcga tcacggctta tgctcagttc tggttccccg atctctccga
     2641 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaacctcg ccaccgtgaa
     2701 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2761 gattgtcgtc ggcctggtca tggtggcgat gcactttcag tcaccgaccg gtgtggaagc
     2821 gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa gtggcttctt
     2881 tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag gtacaacagc
     2941 tgcggaaacc aaagatccag agaaatcact gccacgcgcg attaactcca ttccgatccg
     3001 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     3061 ggtagtcccg gagaaaagcc cgtttgttga actgttcgtg ttggtgggtc tgcctgctgc
     3121 cgccagcgtg attaactttg tggtgctgac ctctgccgct tcttccgcta acagcggcgt
     3181 cttctctacc agtcgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     3241 cgctaaactc tctaagcgcg ccgtacctgc gaaagggctg accttctcct gcatctgcct
     3301 gctcggcggc gtggtgatgc tgtatgtgaa tcccagcgtg attggtgcgt tcacgatgat
     3361 cacgaccgtt tccgcgattc tgtttatgtt cgtctggacg attatccttt gctcgtacct
     3421 ggtgtatcgc aaacagcgtc cgcacctgca tgagaaatca atctacaaga tgccgctcgg
     3481 caagctgatg tgctgggtat gtatggcatt ctttgtgttc gtggtcgtgc tgctgacact
     3541 ggaagatgat acccgccagg cgctgctggt taccccgctg tggtttatcg cactggggct
     3601 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3661 tgctggatgg cgcaatgcca tccagctttt agttcactca cccgccagtg cgcgcgggaa
     3721 cagcacatta ttttccaggc tgatgtgctc catcaggtca tcaatcagtt cattaatgcc
     3781 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtagt
     3841 gtgtttgatt acttccagca gttcacccgc ttcatcgtgc tcgctttcca ttacgctgat
     3901 tggccccatt gcctgcctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3961 tttcatcatg tggctggaaa gctcttcatg cagcatagtc aggtattttg tcagcccttt
     4021 tggcacgctc ggtttgtcgg cgtgaacgcg ttcgactttg ctggcttgca gaatcagctc
     4081 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcga tgatttctgc
     4141 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcgagctttg ccagctcagc
     4201 ttcaatgacc tcaacatcca gctctttacg tgccgccgca cgcgccagcg tttgcttacc
     4261 gccgcagcag taatccatat catatttacg aaacagagcc gaagcgcgag gaatagagag
     4321 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataag
     4381 ataagatgca ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4441 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4501 ggttttgctc tgaccgcaat gatcacgcca accactaaca gtgcgatacc gctcagcgtc
     4561 attagtggcg gcatttgctg gcgtaacaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4621 aaaacaatca gcggcccgag aatcactgtc ggtaatcgct ggctggcgac gttccagcag
     4681 agtgcgccaa cccatgagca aagcacggct attgcaatca tcagactaat aaacaccagc
     4741 ggacgggggc caaaaggtag ggagaagtcc ggcgtttgcg tattcagcca gtaacaagcg
     4801 acgagatagc cgataagcga aaccggcagc gtgaccagcg cctgcgccgt cgcccacatc
     4861 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccatgcc
     4921 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4981 tcggggagtc cgtggtttaa ctcagcaata ttcacactcg ccaggccgat gccaatacaa
     5041 atcagtgccg gggcgagttt tccccacgcg agtttgccgt cgcgctggct ataaagcaga
     5101 ttggcaaaga caggaatgac caccggcagg gtgccgataa tcatcgtgga aacaggcgcg
     5161 ccagtacgtt gaatggcact ggcaaggcag aagtaataaa tgaggttgcc catcatagtg
     5221 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgccccagc
     5281 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccataga ctgcaacatc
     5341 gccgggtatt ccggcacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5401 aacagggcgt acagcacgcc gctaatcatt actggcatcc attggtctgt cagaagaaac
     5461 gttcaggaag cagatatgat tgctcccggc atcccgtcat tatcagagag gatgcgggag
     5521 gatgccactt taattaacta gttattaaca ttaaaaagat ggcttacgct ttcggctaac
     5581 gttgtcgttg ggcggccaat caatttgcta agcgttttgc tgttatcaaa cagaccacct
     5641 ttcgatgcgc caacgtcaga atccgccagc atatccgcca gtccgtcggg cagtccgacg
     5701 cttttcagtg ccgcggcgaa atcggcttcg ctcagatttt gataggtaac ggattttccg
     5761 ctctgtttgg tcagctctgc cgctaactgt gtcaacgtcc aggcactatc gcccgccagt
     5821 tcgtaaacct tgccttcgtg accggcttcg ctaatcacgc gtgccgcagc tgccgcataa
     5881 tctgcccgcg ttgctgaggc gattttgcca tcgcccgccg caccgataaa tacgccgtgt
     5941 tccagtgctg ccggggcgct ggcgaggtag ttttcggtgt accagccgtt gcgcagcagg
     6001 gtgtaaacga tgccagaatc agccagcatt ttctccgtct cgatgtgctc atcggcgagg
     6061 ccgagcgggg aggtatctgc atgtagcagg ctggtataag cgataaattt cacgccagcc
     6121 gcctttgcgg cattaataac attacgatgc tgcggggcac gttgacccac ttcgctggaa
     6181 gagatcagca gtagtttttc cactccctga agtgcagatg tcagtgcggc ttcatcgccg
     6241 tagtcagcct gacgcacggt aatgccttgt gctgccaggg cctgggcttt tgccggatta
     6301 cgaacaatag ccactatttg gctggcagga accgttttca tcaaggattc aataacatag
     6361 tgaccaagtt ggccagtggc accagtaata gcgatcatgg gaagtctcca tcgttttgtc
     6421 ttgtgtttaa atacgctaac acctaaactt acttttagta agtacgtaca aaaaggtaag
     6481 tatgagatga gtcaggttag cctgtcgcaa caactgaaag agggaaatct cttcgctgaa
     6541 cagtgtccgt cgcgcgaggt tttgaaacac gtcaccagcc gttggggggt gttgattctg
     6601 gtggcgctac gcgaaggtac tcatcgcttt agcgacctgc ggcgcaaaat gggcggggtg
     6661 agtgaaaaga tgcttgcgca gtcgttacag gcgctagagc aggatggttt tcttaaccgt
     6721 atcgcgtatc cggtggtgcc gccgcatgtg gaatatagcc tcacgccgct gggcgagcag
     6781 gtgagcgaaa aggttgctgc actggcggac tggattgagt tgaatttgcc cgaggtgttg
     6841 gcggtgcggg atgaacgtgc ggcgtaactg caggatgcgc ggcctacggt gtatgacttg
     6901 taggacggat aagatgcgcc agcatcgcat ccgacaaaag tgtcggatgc ggttgatgat
     6961 ttgtaggccg aaaaagaagt gtattactta ctcaaatcca cctgataaat cgcaaacccg
     7021 atatcatcgg tcgcgacttt attcatcgga tattgccctt tctctttaat aaacgccgcg
     7081 gctttatctg atggagaggt ttcgaaacgg atatccagtt tcttatcgcc agctattggt
     7141 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     7201 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     7261 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     7321 atggcgttcg gatcaattgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7381 gcattaacca tctggcactc gccgtcataa cgggcaggct gggtaacatc aatctgataa
     7441 ttcacaccat caatcacatc aaagttatag gtgcggaaac catcccagtt gatgagtgac
     7501 tgtggtttcg tgctgttagg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7561 tctttcacct ctttaccgct ggctttcacc acaatcagcg tattgggata gagataaaga
     7621 tcggcggcat tacggaaggt caactgacct ttttccacct ccacatagct tgccgggtca
     7681 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcactggcag ttttgccaga
     7741 tccggatcgc cctgaatgta atgctcgaca tacgcttttt gcgcgttgtt caccacctgc
     7801 acggtcggat cgtcctgcac cagtgccaga tagctataga tattgtcggc agatttaccg
     7861 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7921 ttgctgtctt ccgccgcgag ggatttttta ttcgcgatgt cgtaaatcgg ccgtgcttcc
     7981 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccaca
     8041 ccaagatgat cgccccacat gcctggcatt accgccggaa caccattcag cgtgcctttc
     8101 gcgatatcag ccccttcgat atcagcaaaa tctttacctg ggaaaacggc gtgagcatgg
     8161 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     8221 atcactttat acggatcggc agacagcccg gaatgcgcca gaacgacaac gacatcggca
     8281 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     8341 actttcccgg ataaattagc tttatcccag cccatgattt gcggcggcac gacgccaata
     8401 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt gtctttaatt
     8461 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8521 gggaatttcg ctcccgccag cgcatttttc aggtaatcca gaccgtagtt aaactcgtgg
     8581 ttgccaagcg tgccgacggt atagtccagc gtatttaatg ccttatagac cggatgaata
     8641 tcgcctgctt ttaaaccttt cgccgacatg taatctgcaa gcggactccc ctgaatcaaa
     8701 tcgccgttat cgaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8761 gcggtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8821 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8881 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8941 ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc ttacttatag aacagtgaag
     9001 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     9061 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     9121 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     9181 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     9241 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtgc
     9301 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9361 tagacccgct ggatggtact aaagagttta ttaaacgtaa tggcgaattt accgttaaca
     9421 ttgcgctcat cgaccacggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9481 taatgtacag cgtggcagaa ggcaaagcgt ggaaagaaga gtgcggcgtg cgcaagcaga
     9541 ttcaggtccg tgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9601 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9661 tgaaattctg ccttgtggcg gaagggcagg cgcagctgta cccgcgcttc ggaccaacga
     9721 atatttggga caccgccgct ggacatgctg tggccgcagc tgccggagcg cacgttcacg
     9781 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9841 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt ctgccatcct
     9901 gatttctccc aacctaaaaa gttataaata aaaagagatt gtatttaaag tgcaaaaatt
     9961 caattgctaa taagttatat tttaataatg agcgtttttt gatagtttac ttctataatg
    10021 agatatttaa tggcgacata aaataaccaa ataaaataag gtagccatat gttacccagg
    10081 atcagacaca ataattttat tggtgcggtg gagttattcg taaaatcatc gcatacaaaa
    10141 acacattcaa acaatttttt taacaatatc cagcatgcat ttaataaaaa agattggatt
    10201 tcaaattatg atagcctgtt aactttgagg gagttctttc gttgcgcaac tcagattgat
    10261 aaaaatagtt atcaggtact ttcatcaaaa aatgaaactg ttaacgccat ggataagttt
    10321 cttatcagtt tcagccttaa agataacggt accgaatata taatgacatt gcgaggcagt
    10381 ggatttgaat acgaagaaat tcccataaca ataaatgagt ataactcatt catggatttc
    10441 aaaaaccgcg agtttccgtt agaacaaaac agaagattgt atgcctggga tattctgcag
    10501 aaaaaacagt ctgacatacc ggaaaggatt aaaggttata ttcgccaggc gtttggtgac
    10561 gtgtctttag gttatgctgt attggatgat atagtaagta aattaaaacg gggtaaattt
    10621 gacatccaaa taccaggagg tggaattaaa gaatgcgatg gatggtatat ttacgaaaaa
    10681 ataattgatg ataattttgc catagtcata gaatctttgg gctttgcgct taaaatctat
    10741 ggaggggatg aacgttttcg taatggttcc tctgttgttt tggaggatga agattactct
    10801 cttatttata attttctggt taatgcaggt tgccaacaag tcgaattagc cgaacaagtg
    10861 gatgcgatag taagtgcgaa tctggccgca gatagtgata ttaccgaaga aaagatttgc
    10921 gagaaatata aatcgaccat tgaggcgttt aaaaaaaagc aactagcgtt accagtatga
    10981 gttcagcgta agaactcaga ggcttaattc ccctatccca tagataacga taggggaaaa
    11041 ttattttgca gaatttatgt tattgccatt atttgagcaa cttttgcagc aggtccatca
    11101 cttgctgcac ctcttcctgt gtgagcgccc cgtctttggc ccattgcacg cgaccgtctt
    11161 tatccagcac cactacagcg gaactttctt catccagctg ccaggcaccg cgcgcgacgc
    11221 cattgctatc gacaataaac tgcgaccagg gataaagctt tttattactt tccagactgc
    11281 tgcgcacaaa catgccggaa cccggaattg cgtcgtcggt gttaacaatg gtggtggtct
    11341 ggtaacgatc gtgcggtaac tttgctgatt taatcgcttc aatcagcgtc gcgtttttct
    11401 cttttgcaga ggtgcgacca gcaatatgtt gcagtactcg cacttttccc actaactgcg
    11461 cgctgttcca ggttttgtag ctaaactgat ctttatcaag caccaactcg ccacgatcgg
    11521 taatgccaat cggcggcact cgctgaccgg tttcgaactg atgtgcggaa gccatcatcg
    11581 gcaacagcag gcaggtgagt gccagaatct tgcgtagggt catggtgttt ccttcttatg
    11641 atatgcaggt gatccgacca cttgggtctg agttttaatc atatgtgcta tttatcgaat
    11701 ttcccgcaag tgtgatgcca gtttgcggtc aagcgcacaa atcatatgaa aaatgaatgc
    11761 ttatactgaa gaccgcgctt cggtaaaaag ataattctga ataattgtaa cctttaggta
    11821 aaaaaagtta tacgcggtgg aaacattgcc cggatagtct atagtcacta agcattaaaa
    11881 tttgcgcctc ataatagttg ggccgattgt ggcaccgcac aggcgtaata ctcagcagga
    11941 gataacaatg aaaattttcc aacggtacaa cccacttcag gtggcgaagt acgtaaagat
    12001 cctgttccgt ggacggttat acatcaagga cgttggcgct tttgaatttg ataagggtaa
    12061 gattcttatc ccaaaagtga aggataagct gcatttgtca gtgatgtccg aagttaaccg
    12121 tcaggttatg cgtctgcaaa cagagatggc ttaaccaaag tgctatgcag taataagacg
    12181 gctcctgatt caggagccgt tgatgtttct gggggttacg cgacgctttc ttctttatct
    12241 ttagcgtcag gcagttttgg cgaaagggcg gtggccttgc tgtcgatccg ggtcaccagc
    12301 agctggtcga tgcggtagtt atcgatatcc accacttcaa atttgtagcc ggcgaatttc
    12361 accgaatcgg tgcgtttcgg gatcttacgc agcataaaca tcataaagcc gccaatggtt
    12421 tcgtagttgc ccgactgcgg gaactcgtca atatccagca cgcgcatgac gtcgtcaatc
    12481 ggagtgccgc cgtcaatcag ccatgagttc tcatcacggg cgacaatctg ctcttccagc
    12541 ccctgaccga ccagatcgcc catcagcgtg gtcatcacgt cgttgagggt gatgatcccc
    12601 accaccagcg cgtactcgtt catgatcacc gcgaagtctt cacctgcggt tttaaaactt
    12661 tccaacgcct ctgaaagggt taacgtatcc ggcacaatca gcgtgttgcg aatttgtacg
    12721 ccgctgttca gtgccaggct ttggttagcc agcacgcggt tcagcaggtc tttagaatcg
    12781 acatagccga tgatgtggtc aatatcttca ttacagacga ggaacttaga gtgcggatgt
    12841 tccgccacct tattcttcag actttgctca tcttcgtgga gatcaaacca aatcacgttt
    12901 tcacgcggtg tcattgaaga cggaacggta cgagattcca gctcaaagac gttttcaatc
    12961 agctcgtgtt cctgtttacg taacacgccc gccagcgcac cggcttccac taccgcgtaa
    13021 atatcatcag aagtgatgtc atctttacgt accattggca gtttgaaaat acggaagatc
    13081 atgttcgcca ggccgttgaa gaaccacacc agcggggtgc aaacgtacag gcagaagcgc
    13141 atcgggttga tgatacgcaa agccacagct tctggcgcaa tcataccgat gcgtttcggg
    13201 gttaaatccg caaacaggat aaacatgcca gtcactaacg agaaagagag aataaagctc
    13261 agttgctcag agagttctgc cgacatatag cgggagaaca ggctgtgaaa agctggagaa
    13321 aatgccgcat caccgacgat accgccgaga atcgccactg cgttcagacc gatttggacc
    13381 acggtaaaga acatgccggg attttcctgc atattcagaa cgcgttgggc atttatattg
    13441 ccttcatcag ccagcagttt aagtttgatt ttgcgtgaag cggcaagcga gatctcagac
    13501 atcgagaaga acgcacttac agcgatcaag cagagtatga ctaaaatact gtttaacata
    13561 tcttatccga cctttcaggc cagatcctcg gaaggggaag ttgattaatt tgtgtgaaat
    13621 acacattgaa agccgattcg aagaaagtga accggcattt tcagtggcta gtatagcgta
    13681 aggtactgta aagccgccag agggttaaaa ttcagactgc tgataaatgc aaaactgcct
    13741 gatgcgctat gccaatcagg cctacgcagt ccatgcaata tattgaattt ggacgatttt
    13801 gtagacctgg taaggcgttt acgccgcatc cggcataaac aaaacgcact ttatcaacaa
    13861 tctacatttg tacccgtaac gctatgcttc cggcggcaga cagacgccaa ttccgccaat
    13921 tccacagtaa ccatacgggt ttttatgcag atattgctgg tggtcatctt cggcataata
    13981 aaacggtgtg gcgttagcga tttccgtggt gatgcgacga tcgtcatcgg cggcaagcat
    14041 tgccgcctga aaacgttcca gactggcgcg agctgcggca tcctgttctg gggtcagcgg
    14101 ataaatcgct gaacgatact gcgtgccgtg gtcattgccc tgacgcattc cctgggcggg
    14161 atcgtgattc tcccaaaata cttgtagcaa ctgttcatag ctgatgacgg aaggatcgta
    14221 aacaatgcgt accgcttcgg catgacccgt atcaccggag cacacttccc gataagtcgg
    14281 atttggcgta tagccgccgg tatagcctgc ggcggtgctg taaacgccgg gtaactgcca
    14341 gaacagacgc tccacacccc agaaacaacc catcgcaaaa atggcaatct ccattccgtc
    14401 aggtacattg gtcattgagt gaccgttgac cgcatgcagt gtggctacgg gcatcggggt
    14461 gttacgtcca ggcagggcat cggcggggga aaccagatgc tttttatcga ataaactcat
    14521 ggtgtcgctc tcccgaaaat cggtcattgg ggttaaggtt gtaacaagag gcgtatttgc
    14581 acacaataac caccgtgaat aggtctaaag taaaacataa gaaatatttg gggtttagtc
    14641 tgctttttaa tccatattac tggatttttg ttaagccgtt taacggcgtt ccaggggcag
    14701 gaaaaaagga tattcaggag aaaatgtgcg ctatatccga cagttatgct gtgtaagctt
    14761 actctgctta agcggatctg ccgtcgccgc gaacgtccgt ctacaggtcg aggggttatc
    14821 gggacagctg gaaaagaacg ttcgcgcgca gctttctacg attgaaagtg atgaagtgac
    14881 gccagaccgt cgctttcgcg cacgcgtcga tgatgccatc cgcgaaggtc tgaaagcgct
    14941 gggttattac cagccgacca ttgaatttga tctccgtcca ccgccaaaga aagggcggca
    15001 ggtattgatc gccaaagtca cgccaggcgt gccggtgtta attggcggca cagatgtggt
    15061 attgcgcggc ggcgcgcgga ccgataaaga ctatttgaaa ttgctcgata ctcgcccggc
    15121 tattggtacg gtactgaacc agggcgatta tgaaaatttc aaaaagtcct taaccagcat
    15181 tgcgttgcgt aaaggttatt tcgatagcga atttaccaaa gcgcagctgg gcatcgcgct
    15241 cggcctgcat aaagccttct gggatattga ttataacagt ggcgaacgct accgctttgg
    15301 gcatgtgacc tttgaaggat cacaaatccg cgatgaatac ctgcaaaatc tggtgccgtt
    15361 caaagagggc gatgagtacg aatcgaaaga tctggcggaa ctaaaccgcc gactctctgc
    15421 caccggctgg tttaattcgg tggtggtggc accacaattt gataaagcgc gcgaaacgaa
    15481 agtattaccg ttgacgggcg tggtttcgcc gcgcacagaa aacaccatcg aaaccggtgt
    15541 cggttactct acggacgtgg gaccgcgcgt gaaagcgacg tggaaaaagc cgtggatgaa
    15601 ctcatacggt cacagtctga ccaccagtac cagtatttcc gcgccggaac agaccctcga
    15661 cttcagctat aaaatgccgc tgctgaagaa tccactggaa caatattatt tggtgcaggg
    15721 cggttttaag cgcactgacc tgaacgatac cgagtctgac tccactacgc tggtggcttc
    15781 tcgctactgg gatctttcca gcggctggca gcgtgccatt aacctgcgct ggagtctcga
    15841 ccactttacc cagggtgaaa ttaccaacac cacgatgctg ttttatccgg gggtgatgat
    15901 tagccgcacg cgttctcgtg gtggcctgat gccaacctgg ggcgactcgc aacgttactc
    15961 tatcgactac tccaacacgg cctggggctc agatgtcgat ttctccgttt tccaggcaca
    16021 aaacgtctgg atccgcacac tgtacgaccg ccatcgcttt gtgacacgcg gcacgctggg
    16081 ctggattgaa acgggtgatt tcgacaaagt accgccggat ctgcgtttct tcgccggggg
    16141 cgatcgcagt attcgcggct acaaatacaa atctatcgcc ccgaaatacg ccaacggtga
    16201 cctgaaaggg gcctcgaagt tgataaccgg atcgctggag taccagtaca acgtgaccgg
    16261 aaaatggtgg ggcgcggtgt ttgtcgatag tggcgaagcg gtaagcgata ttcgccgcag
    16321 cgactttaaa accggtaccg gggtcggcgt gcgctgggaa tcgccggtcg ggccaatcaa
    16381 actcgatttt gccgtaccgg tcgcggataa agacgaacac gggttacagt tttacatcgg
    16441 tctggggcca gaattatgag tttatggaaa aaaatcagcc tcggcgtggt tatcgttatc
    16501 ttactgttgc tgggatcggt ggcgtttctg gtgggcacca ccagcggcct gcatctggta
    16561 tttaaagcgg cggatcgctg ggtaccagga ctggatattg gcaaagtcac cggcggctgg
    16621 cgcgatctca ccttgtctga cgttcgttat gagcagccag gcgtggcggt aaaagcgggc
    16681 aatctgcatc tggcggtcgg gcttgagtgc ctgtggaaca gtagcgtttg tattaatgac
    16741 ctggcgctga aagacattca ggtcaacatc gacagtaaaa aaatgcctcc ttctgaacag
    16801 gttgaagaag aggaagatag cggtccgctg gatctctcta cgccgtatcc catcaccctg
    16861 acacgggtgg cgctggataa cgtcaacatc aagattgatg acaccacggt atcggtgatg
    16921 gacttcacct ccggcctgaa ctggcaggag aaaaccctga ctctgaaacc gacgtcgctg
    16981 aaaggcctgt tgattgcttt gccgaaagtg gcggaagtgg cgcaggaaga agttgtcgaa
    17041 ccgaaaattg aaaatccgca gccggatgaa aagccgctcg gcgaaacgct gaaagatctc
    17101 ttttctcgcc cggtattgcc ggaaatgacc gacgtgcatc tgccgctcaa cctgaacatt
    17161 gaagagttta agggcgagca actgcgcgtg acgggcgaca cagacatcac cgtgcgcacc
    17221 atgctgctga aagtgagcag tatcgacggc aataccaaac tggacgcgct ggatatcgac
    17281 tccaaccagg ggattgtcaa cgccagcggc acggcgcagc tgtcagacaa ctggccggtg
    17341 gatatcaccc tcaacagcac actgaacgtg gagccgttga aaggtgaaaa ggtgaagctg
    17401 aaagtgggtg gcgcgctgcg cgaacagctg gagataggcg ttaacctttc cggcccggtg
    17461 gatatggatt tacgcgccca gacgcgactg gcggaagccg gattgccgct caacgtggaa
    17521 gtgaacagca aacagcttta ctggccgttc actggagaga agcagtatca ggcggatgat
    17581 ctgaaactga aacttaccgg caaaatgacc gattacacgc tctctatgcg tacggcagtg
    17641 aagggacagg agatcccgcc agccaccatt acccttgatg ccaaaggtaa tgaacagcag
    17701 gtcaatctcg acaaactcac cgtcgcggcg ctggaaggga aaactgaact caaagcgttg
    17761 ctcgactggc agcaggccat tagttggcgc ggtgagctaa cgcttaacgg cattaacacc
    17821 gccaaagagt tcccggagtg gccgtcgaaa ctcaatggct tgattaaaac ccgcggtagc
    17881 ctgtacggcg gcacctggca gatggaggtg ccagaactga agctgaccgg taacgtcaaa
    17941 cagaacaaag tgaacgttga cggcacgctg aaaggcaaca gttatatgca gtggatgatc
    18001 ccagggcttc atctggaact ggggccaaac agtgccgaag tgaaaggcga gctgggggta
    18061 aaagatctca atcttgatgc caccatcaac gcgccggggc tggataacgc gctgccgggg
    18121 cttggcggta cagcgaaagg gctggtgaaa gtacgcggca cggtggaagc gccacaacta
    18181 ctggcggata tcaccgcgcg cggcctgcgc tggcaggaac tttccgtggc gcaggttcgc
    18241 gtggaaggcg atatcaaatc caccgatcag attgcgggta aacttgacgt acgcgttgag
    18301 caaatctcgc agcccgatgt gagtatcaac ctcgtcaccc tgaatgccaa aggcagcgaa
    18361 aaacagcacg agttacagtt gcggattcag ggcgagccgg tttccgggca gcttaatctg
    18421 gcaggaagtt ttgatcgcaa agaagaacgc tggaagggaa ctcttagcaa tacccgcttc
    18481 cagacgccgg tcggcccgtg gtcgctgacc cgcgatattg cgctggatta ccgcaataag
    18541 gagcaaaaaa tcagcatcgg gccacactgt tggcttaacc cgaatgcgga actatgcgtg
    18601 ccgcaaacta tcgatgcggg ggccgaaggg cgtgcggtgg tgaatctcaa ccgcttcgac
    18661 ctggcgatgc tgaaaccgtt tatgccagaa accactcagg ccagcggtat cttcacgggt
    18721 aaagcggatg tcgcctggga caccacgaaa gaggggctgc cgcagggcag tatcaccctt
    18781 tcggggcgta acgtgcaggt aacgcaaacc gtcaacgacg cggcgctgcc cgtggcgttt
    18841 cagacactga atctgacggc ggaattgcgt aacaaccgtg ccgaattggg ctggaccatc
    18901 cgcctgacca ataacggcca gtttgatgga caggtgcagg tgaccgatcc gcaaggccgc
    18961 cgtaatcttg gtggcaacgt caatatccgt aacttcaacc ttgcgatgat aaaccccatc
    19021 tttacccgtg gggaaaaagc agcggggatg gtgagtgcca acttgcgtct gggtggtgat
    19081 gtgcaaagcc cgcagttgtt tgggcagcta caggttacgg gtgtggatat cgacggcaac
    19141 tttatgccgt ttgatatgca gccgagccag cttgcggtca actttaacgg tatgcgctcg
    19201 acgcttgccg gtaccgtgcg gacccagcag ggtgagatct acctgaacgg cgacgccgac
    19261 tggagccaga ttgaaaactg gcgggcgcgg gtaacagcga agggcagtaa agtgcgtatc
    19321 accgtgccgc cgatggtacg aatggatgta tcaccagatg ttgtattcga ggctacacca
    19381 aacctgttta cccttgatgg tcgcgtggat gtcccgtggg cgcgcatcgt ggtgcatgag
    19441 ctgccggaaa gcgcggtggg cgtctccagc gatgtggtga tgcttaacga taacctgcaa
    19501 ccggaagagg caaaaacggc gtcgattccg attaacagca acctgattgt ccacgtcggc
    19561 aacaatgtgc gcattgacgc ctttggcctg aaagcgcggc tgacgggcga tctcaacgtc
    19621 gttcaggaca aacaagggct gggcctgaac gggcagatca acatccctga agggcgcttc
    19681 catgcctatg gtcaggatct gattgtgcgt aagggtgagt tactgttctc tggtccgccg
    19741 gatcaaccgt atcttaatat cgaagctatt cgtaacccgg atgctacaga agacgacgta
    19801 atcgccggag ttcgcgtcac tggtctggca gacgaaccga aagcggagat cttctctgac
    19861 ccggccatgt cgcaacaggc tgccttgtct tatttgctac gtggacaagg gctggagagc
    19921 gatcagagcg acagtgcggc gatgacctca atgctgattg gtctgggggt tgcacaaagt
    19981 ggtcagattg tgggtaaaat cggcgagacg tttggcgtaa gcaatttagc gctcgacacc
    20041 cagggagtag gcgactcctc ccaggtggtg gtcagcggct atgtattgcc aggtctgcaa
    20101 gtgaaatacg gcgtgggtat atttgactct atagcaacac tcacgttacg ttatcgcctg
    20161 atgcctaagc tatatctgga agccgtgtct ggtgtagacc aggcactgga tttgctctat
    20221 cagttcgagt tttagcaatg cgaatatttg tctacggcag tttacgccac aaacaaggca
    20281 acagtcactg gatgaccaat gcccagttac tgggcgattt cagtatcgat aactaccagt
    20341 tgtatagcct gggccactat ccaggcgcag ttccggggaa cggaacagta cacggtgaag
    20401 tttatcgtat tgacaacgcc acgctggccg aacttgatgc cttgcgcacc aggggcggtg
    20461 aatacgcgcg ccagttgatt cagacgccgt acgggagtgc atggatgtac gtttatcaac
    20521 gacccgtcga tggattaaag ctaattgaaa gcggcgactg gttagacagg gataagtaac
    20581 catacgcata cgccaccttc gggtggcgtt gttttttgcg aaacgactcg cattctgttt
    20641 tgtaattccc tcaccttttg cttttctctc cgagccgctt tccatatctg ttaacgcata
    20701 aaaaactctg ctggcgttca caaatgcgca ggggtaaaac gtttcctgta gcaccgtgag
    20761 ttatactttg tataacttaa ggaggtgcag atgcgtatta ccataaaaag atgggggaac
    20821 agttcaggta tggtcattcc taatgttgtg atgaaagaac ttaacttacg gccggggcag
    20881 agcgtggagg cgcaagtgag caacaatcaa ctgattctga cacccatctc caggcgctac
    20941 tcgcttgatg aactgctggc acagtgtgac atgaacgcca cggaacttag cgagcaggat
    21001 gtctggggta aatccacccc tgcgggtgac gaaatatggt aaagaaaagt gaatttgaac
    21061 ggggagacat tgtgctggtt ggctttgatc cagcaagcgg ccatgaacag caaggtgctg
    21121 gtcgacctgc gcttgtgctc tccgttcaag cctttaatca actgggaatg acgctggtgg
    21181 cccccattac gcagggcgga aattttgccc gttatgccgg atttagcgtt cctttacatt
    21241 gcgaagaagg cgatgtgcac ggcgtggtgc tggtgaatca ggtgcggatg atggatctac
    21301 gcgcccggct ggcaaagcgt attggtctgg ctgcgggtga ggtggtggaa gaggcgttat
    21361 tacgtttgca ggcggtggtg gaataaggtg tgtttattta tcgcgggcat aaaaaacccc
    21421 tactaaccga agcccggcgt tcagggttat tacgctagaa gaacttattt attctttgcg
    21481 cgctcgaagg aggcaacgat ttcagcttta gcggcttctg cgttttccca gccttcaact
    21541 ttcacccact tgcctttttc gaggtctttg tagtgctcga agaagtgagc gatctgtgct
    21601 ttcagcagtt caggcagatc gttaacgtct ttaatgtgat cgtattcttt gctcagcttg
    21661 ctgtgcggaa ccgcaaccag tttcgcatct tcaccggctt cgtcggtcat tttcagaacg
    21721 ccaacaggac ggcaacggat cacagaaccc ggctgcagcg ggtacggagt cgggaccagt
    21781 acgtcaaccg ggtcaccgtc cagagacagg gtgtggttga tgtaaccgta gttgcacgga
    21841 tagaacatcg cggtggacat gaagcggtca acgaacagtg cgccgctctc tttgtcgatt
    21901 tcgtatttga tcggatctgc gttagccggg atctcaataa caacgtagat gtcttccggc
    21961 agatctttac ccgcagggac gttgagtaag ctcatgtctg tttcctttaa aaatatgtgg
    22021 caaacaagtg ccgagtatta tagccaactc gcgccgaatg tcttcgcttg ttttcttctt
    22081 cattttcctt tttagccagt tttaagatgg tttaaagagc aggaaaatcc ataaccgtag
    22141 ccgcattttt catagtgaga tgaaagcgat tacaaacttg tgattaacgt tttatttact
    22201 tttttgaagt gtgatgtaac gcaatccgtt acataacgaa ttgtctatag ttttttcgcg
    22261 aacatctttt caccaataat aattaccccg acgaggacaa ccctatgtgg aaacgcttac
    22321 ttgtagtctc tgcagtctcg gcagccatgt cgtctatggc gttggccgct ccattaaccg
    22381 ttggattttc gcaggtcgga tcggaatccg gctggcgcgc cgcagaaacc aatgtggcga
    22441 aaagtgaagc cgaaaagcgc ggaattacgt tgaaaattgc cgatggtcag caaaagcagg
    22501 aaaaccagat taaagcggta cgttccttcg tcgcgcaagg ggtggatgcg atctttatcg
    22561 ctccagtggt agcgaccggt tgggagccgg tattaaaaga ggcgaaagat gccgaaatcc
    22621 cggtcttctt gcttgaccgt tccatcgatg tgaaagacaa atctctctat atgaccactg
    22681 tcaccgccga caacatcctc gaaggcaagt tgattggtga ctggctggta aaagaagtga
    22741 atggcaaaca atgcaacgtg gtggagctgc agggcaccgt tggggccagc gtcgccattg
    22801 accgtaagaa aggctttgcc gaagccatta agaatgcgcc aaatatcaaa attatccgct
    22861 cgcagtcagg tgacttcacc cgcagtaaag gcaaagaagt catggagagc tttatcaaag
    22921 cggaaaacaa cggcaaaaat atctgtatgg tttacgccca taacgatgac atggtgattg
    22981 gtgcaattca ggcaattaaa gaagcgggcc tgaagccggg taaagatatc ctcacgggtt
    23041 ccattgatgg cgtaccggat atctacaaag cgatgattga tggcgaagcg aacgccagtg
    23101 ttgaactgac gccgaacatg gcaggtcctg ctttcgacgc gctggagaaa tacaaaaaag
    23161 acggcaccat gcctgaaaag ctgacgctga ccaaatccac cctttatctg cctgataccg
    23221 caaaagaaga gttagagaag aagaagaata tggggtattg aggggcatcg atgcctgatg
    23281 ccgacttgtg ggccgccgga taagacattc acgccgcatc cggcggttgt gcgagatgcc
    23341 tgatgcgacg ctgttgtgtc ttatctggcg cacgctttac aacgggggaa actatgaata
    23401 ctaaccaaca ccaggaaatc cttcgcaccg aaggattaag taaatttttc cccggtgtca
    23461 aagcgttaga caacgttgat ttcagcctgc gccgtggtga aatcatggcg ctgctcggtg
    23521 aaaacggggc gggaaaatca acgctaatca aagcattaac cggtgtctac cacgccgatc
    23581 gtggcaccat ctggctggaa ggccagacta tctcaccgaa aaataccgcc catgcacaac
    23641 aactcggcat cggcaccgtc tatcaggaag tcaacctgct acccaatatg tcggtcgctg
    23701 ataatctatt cataggtcgc gaacccaaac gtttcggcct tctacgccgt aaagagatgg
    23761 aaaagcgcgc caccgaactg atggcatctt acggtttctc cctcgacgtg cgcgaaccgc
    23821 tcaaccgctt ttcagtggcg atgcagcaaa tcgtcgccat ttgccgggct atcgatctct
    23881 ctgccaaagt gctgatcctc gatgaaccca ctgccagtct cgacactcag gaagtagagt
    23941 tactgtttgg cctgatgcgt cagttgcgcg atcgcggcgt cagcctgatc tttgtcactc
    24001 actttctcga tcaggtctat caggtcagcg atcggatcac cgtcttacgc aacggcagtt
    24061 tcgtaggctg tcgggaaacc cgcgagctac cgcagatcga actggtaaaa atgatgctgg
    24121 ggcgcgagct ggacacccac gcgctacagc gtgccgggcg aacattgttg agcgacaaat
    24181 ccgttgccgc gttcaaaaat tacggcaaaa aaggaaccat cgcaccgttt gatctcgaag
    24241 tgcgccccgg cgagatcgtc ggcctggcag gcttgctggg atcaggacgt accgaaaccg
    24301 ccgaagtgat cttcgggatc aaacctgctg acagcggcac ggcgttgatc aaaggcaaac
    24361 agcaaaccct gcgatcgcca catcaggctt cggtactggg tatcggcttc tgcccggaag
    24421 acaggaaaac cgatggcatc atcgccgccg cctcggtgcg ggaaaatatc atcctcgctc
    24481 tccaggccca gcgcggctgg ctacgaccga tttcccgcaa agaacagcaa gagattgccc
    24541 aacgctttat ccgccagctt ggcattcgca caccttcaac tgaacaaccg attgaatttc
    24601 tctccggcgg caatcagcaa aaagtgttgc tttcacgttg gctactgacc cgaccgcaat
    24661 ttctgatcct cgatgagcca acgcgcggca ttgacgttgg tgcgcatgcc gagatcatcc
    24721 gcctgattga aacgctgtgc gctgatggtc tggcgctgct ggtgatctcc tctgaactgg
    24781 aagagctggt gggctatgcc gaccgggtga ttatcatgcg cgatcgcaaa caggtggcgg
    24841 agatcccgct ggcagagctt tccgttccgg cgatcatgaa cgccattgcg gcgtaaggag
    24901 aacagtgtga tgcctcaatc tctcccggac actacgccgc cgaaaaggcg ctttcgctgg
    24961 ccaacaggaa tgccgcaact ggtagcgcta ttgctggtgc tgctggtcga cagtctggtg
    25021 gccccgcatt tctggcaggt tgtgcttcag gatgggcgtt tgttcggtag ccctatcgac
    25081 atccttaacc gtgcagctcc cgttgcgtta ctggcgattg gcatgacgct ggtgatcgcc
    25141 actggtggta tcgatctctc cgtaggggcg gtgatggcta tcgccggagc cacaacggct
    25201 gcgatgacgg tcgcgggatt cagcctgccg attgttttgt taagcgccct gggcactggc
    25261 atcctggcgg gattgtggaa cggcatactg gtagcgatcc tcaaaattca gccgtttgtt
    25321 gccaccctga tcctgatggt cgccgggcgc ggcgtggcgc aactgatcac ctccggacag
    25381 atcgtcacgt ttaactcgcc ggatctctca tggtttggca gcggatcgct gttgttcctg
    25441 ccaacgccgg tcattatcgc ggtgctgacg cttctcctgt tctggctgtt gacccgcaaa
    25501 acggcgctgg ggatgtttat cgaagccgtt ggtatcaaca ttcgggcggc aaaaaatgcc
    25561 ggagtcaaca cgcgaatcat cgtcatgctc acctacgtgt tgagcgggct gtgtgcggcg
    25621 attgcgggca ttatcgtggc ggcggatatt cgcggtgccg atgccaacaa cgccggatta
    25681 tggctggagc tggacgccat tcttgcggtg gtgattggcg gcggatcgct gatgggcggg
    25741 cgttttaacc tgctgctctc agtggtaggg gcgctgatta ttcagggaat gaacaccgga
    25801 attttgcttt cgggctttcc gccagagatg aaccaggtgg tgaaagcggt agtggtgctt
    25861 tgcgtgctga ttgttcagtc gcaacgcttt atcagtctga ttaaaggagt gcgtagccgt
    25921 gataaaacgt aacctaccgc ttatgatcac catcggcgtc tttgtgctgg gctatctcta
    25981 ctgcctgacg cagtttcccg gtttcgcttc tacaagagtg atctgcaata tcctgaccga
    26041 taatgctttc cttgggatca tcgctgttgg catgaccttt gtgatcctct ccggtgggat
    26101 cgatctctcc gtcggttcgg tgatcgcctt taccggcgtg tttctggcaa aagtgattgg
    26161 cgatttcggc ctctcgccgt tgctggcgtt tccgctagtg ctggtgatgg gctgtgcctt
    26221 cggcgcattt atggggcttt tgatcgacgc cctgaagatc ccggcgttta ttattactct
    26281 ggcgggaatg ttctttttgc gtggcgtcag ctatctcgtt tcggaagagt cgatcccgat
    26341 aaatcatcct gtttatgaca cgctctcaag ccttgcatgg aaaatccctg gcggcggtcg
    26401 cttaagtgcg atgggactgc tgatgctggc ggtggtggtt atcggcattt tcctcgcgca
    26461 tcgtacccgt tttggtaatc aggtatacgc cattggcggc aacgcgacgt cggcaaacct
    26521 gatggggatt tccactcgca gcactacaat tcgcatttat atgctctcta ccggactggc
    26581 gacactggca ggaattgtct tctcgattta tacccaggca ggatatgcgc tggcgggcgt
    26641 aggtgtggaa ctggacgcca tcgcgtcggt ggtgattggt ggtacgttgt taagtggtgg
    26701 ggtaggaacg gtgctgggaa cgctttttgg cgtggcgatt cagggactga ttcagactta
    26761 cataaacttt gatggcacgc tgagctcatg gtggacgaaa atcgccatcg gtattttgtt
    26821 gtttattttt atagcattac agcgtggatt aacggtgctg tgggagaatc gtcagagttc
    26881 gcctgtgaca agagtcaaca ttgcgcagcg ataaaaacgc ctctccgtgt ggagaggcgc
    26941 aggagattac gcgtccggga actcacgaat aaagcgttcg acatcttcaa ccatatggtc
    27001 gttgccgaca aagaatgaac ggcgctggtg cagggtttcc gggatgatat ccagaatacg
    27061 ctctttgcca tcgctcgctt taccgcccgc ttgttccgcc aggaatgcca tcgggttgca
    27121 ctcatacagt aaacgcagtt taccgtccgg gtggctggcg gtgcttgggt agagataaat
    27181 accgcctttc agcaggttac ggtggaaatc cgcgaccagt gaaccgatat aacgtgaggt
    27241 ataagggcgg ttggtggact tatcttcttc ctggcagaat ttaatgtact tcttcacccc
    27301 gttcggaaac ttaatgtagt ttccttcgtt gatggagtag gttttgcctt tctccgggaa
    27361 gcgcatccgt tcctggcaca ggcagaaaac gcccagcgaa ggatcgtaag taaaggcgtg
    27421 aacaccgcat ccggtggtgt aaaccagcat ggtagaggag ccgtatacca cgtaacctgc
    27481 cgcaacctgt ttgttaccag gctggaggaa atcttcttcc gttaccggcg tgccgacagg
    27541 cgtaacgcgg cggtagatgg agaaaatggt accgacagag acgttaacat cgatgttgga
    27601 cgagccatcc agggggtcca tcagcaccac gtattttgcg tgttcacagc cttcaaagac
    27661 gacaatctca tcttcttctt cagaggcaat gcccgcaacg atatcgcgtg ctttcagtgc
    27721 ggctttcagt ttttcattag cgaacaagtc gagtttctgc tgaacctcgc cctgcacgtt
    27781 ctcagcaccg ctggcaccca ggatatcaac cagtcctgct ttgttgatat cgcgatggat
    27841 aatcttggcg cccagtttta ttgccgacag caaagcagtg agctcaccgg tagcatgaga
    27901 aaactcgtgc tgcttttcga caataaattc acctaacgtt ttcataaaac tttccctgca
    27961 atgtttatgg agtaaagcga ccgcaacaat cttaacaaat aatctcaatg ttgcgctcag
    28021 gtgaatcgcg ccagcaaatt gcggattatc ctgaaatgcg tttctcactt gcccgacata
    28081 tgcgtaaaat gagcggcaga ttaaaaaagg atagtgacgt atgcgcattc atattttagg
    28141 aatttgtggc acgtttatgg gcggtctggc gatgctggcg cgccagttag gccatgaagt
    28201 aacgggttcg gacgccaatg tgtatccgcc gatgagcacc ttacttgaga agcaaggcat
    28261 tgaactgatt cagggttacg atgccagcca gctcgaaccg cagccggatc tggtgattat
    28321 tggcaacgcc atgacccgtg gaaatccgtg tgtggaagcg gtactggaaa aaaacatccc
    28381 ttatatgtca ggtccgcagt ggctgcacga ttttgtactg cgtgaccgtt gggtgctggc
    28441 cgtcgccggt acacacggta aaacgactac agcgggaatg gcgacctgga ttctggaaca
    28501 gtgcggttac aaacccggat ttgtgatcgg cggtgtgccg gggaactttg aggtctcggc
    28561 gcgtctgggc gaaagcgact tctttgttat cgaagcggat gagtatgact gcgccttctt
    28621 cgacaaacgc tctaaatttg tccattactg cccgcgtacg ctgatcctca acaaccttga
    28681 gttcgatcac gccgatatct ttgacgacct gaaagcgatc cagaaacagt tccaccatct
    28741 ggtgcgtatc gttccggggc agggccgtat tatctggccg gaaaacgaca tcaacctgaa
    28801 acagaccatg gcgatgggct gctggagcga gcaggagctg gtgggcgagc agggtcactg
    28861 gcaggcgaaa aagctaacca ccgatgcttc tgaatgggaa gtcttgctgg atggcgaaaa
    28921 agtgggcgaa gtgaaatggt cgctggtagg cgaacataat atgcacaatg gcctgatggc
    28981 gattgcagcg gctcgccatg ttggtgtagc gccggcagat gccgctaacg cgctgggttc
    29041 gtttattaac gctcgtcgcc gtctggagtt gcgtggtgaa gcgaatggcg tcacggtata
    29101 tgacgatttc gcccatcacc caacggcgat tctggcaacg ctggcggcgc tgcgtggcaa
    29161 agttggtggt acggcgcgca ttattgcggt gctggaaccg cgctcgaata ccatgaaaat
    29221 ggggatctgc aaagacgatc tggcaccttc attaggtcgt gccgatgaag tcttcctgct
    29281 gcaaccggcg catattccgt ggcaggtggc agaagtggca gaagcctgcg ttcagcctgc
    29341 acactggagt ggcgatgtcg atgcgctggc agatatggtg gtgaaaaccg ctcagcctgg
    29401 cgaccatatt ctggtgatga gcaacggcgg ttttggtggg atccatcaga aactgctgga
    29461 tggactggcg aagaaagcgg aagccgcgca gtaattctga agccttgcat gtgtgtgtat
    29521 gccggataaa gcgtttacgc cgcatccggc atttgagcat agtgcctgat gcgacgcttg
    29581 atgcgtctta tcaggcctac aggtgcaccg catccggcaa accatactca catcaacaac
    29641 gaaaattacc cttcgttctc cgctaactcg cgcagatact ggaaaatctg gcgtgcggat
    29701 ttcggcggct tattcccttc tttctctttc ttcgcgttgc ggatcagagt acgcagttgc
    29761 tgacgatcgg catcgggcca cagatttaat acttcagcga tggcatcatc accctggtcg
    29821 atcagacgat cgcgcaggtt ttccagttta tggaacaaca ccacctgctg gttgtggcgg
    29881 tttttcagct tatccagcgc ctgacgaata ggctctacgt cgcgctggcg cagcatctta
    29941 ccgatgagct gcaactggcg gcggcgacct tccatcttaa tacgctgggc gagctcaata
    30001 gccgcgcgca gatccgcatc taacgggatc ttatccagcg cgtttttccc cagatcaaca
    30061 atttccgcgc caaggcgttt tagctcctcg gcatcgcgtt taatttcact tttactgacc
    30121 cagataattt catcgtcttc gtcttcgatg tcatcaccgg gaacgtcgtc gagccagtct
    30181 tcgggctgct tagtcatctc aggctcctta aaaaaagagg ctaatgttac cagttaagat
    30241 gcgcactgaa aaacggttct ctgttagact tcagagaaac tctctacatt atggcacttg
    30301 caatgaaagt aatctctcaa gttgaagcgc agcgcaagat tctggaagaa gcagtttcca
    30361 ctgcgctgga gttggcctca ggcaaatcgg acggtgcgga agttgccgtc agcaagacca
    30421 ccggcattag cgtaagcacg cgttatggtg aagtggagaa tgttgaattc aatagcgatg
    30481 gcgcgctggg gatcactgtt tatcaccaga accgcaaagg tagcgcatcg tctaccgatt
    30541 taagcccgca ggccattgct cgcaccgtac aggcggcgct agatattgcc cgttatacct
    30601 cgccagatcc ctgtgccggc gtggcagaca aagagctgct ggcctttgac gcaccggatc
    30661 tcgacttgtt ccaccctgcg gaagtttccc cggatgaagc cattgaactg gcggcccgcg
    30721 cagaacaggc agcattgcag gcggacaaac gcatcaccaa taccgaaggt ggcagcttta
    30781 acagccacta cggtgtcaaa gtttttggca acagccacgg catgttgcag ggttactgct
    30841 caacgcgtca ttcgctctcc agctgtgtaa ttgctgaaga aaatggcgat atggagcgtg
    30901 attacgccta caccattggt cgtgcgatga gcgatctgca aacgccagag tgggttgggg
    30961 ccgactgtgc tcgccgtact ttatcgcgcc tgtcaccgcg taaactctcc accatgaaag
    31021 cgccggtcat ttttgccaat gaagtggcaa ccgggctttt tgggcatctg gtgggggcga
    31081 tagcgggtgg atcggtttac cgtaaatcta ccttcctgct ggattcgctg ggtaaacaaa
    31141 ttctgccgga ctggctgacc attgaagagc atccgcatct gctgaaaggg ctggcgtcga
    31201 cgccattcga cagcgaaggt gtgcgcaccg agcgccgcga tatcgtcaaa gatggcatcc
    31261 tgactcagtg gctgctgacc agctattcgg cgcggaaact ggggctgaaa agcaccggac
    31321 atgcgggcgg tattcacaac tggcggattg ccggacaagg gctaagcttc gaacaaatgc
    31381 tcaaagagat gggcaccggg ctggtggtga cggagctgat gggccagggc gtaagtgcca
    31441 ttaccggtga ttattcccgt ggtgcagcgg gcttctgggt agagaatggc gaaattcagt
    31501 atccggtgag tgaaatcacc atcgcaggta atttaaaaga tatgtggcgc aatattgtca
    31561 ccgtcggtaa cgatattgaa acacgcagta atatacagtg tggttctgtg ctgttgccgg
    31621 agatgaaaat cgccgggcag taaaaaaact ggcgcgacct gccgcgctat aaactaaaaa
    31681 attacacaaa atcattcgca ctgcatcgag acggaaagcg aataagagcc ggggagcgta
    31741 ctcgtagtac gtgactcggg cgaatgagcg tagccgtcga tgaggcagtg tgaaggatga
    31801 agtgtaaata aaaaaggaag tgagcaatgc gtaaaagcct gttagctatt cttgcagtct
    31861 cctcattggt attcagttcg gcgtcgtttg ccgctgatct ggaagacaat atggaaaccc
    31921 tcaacgacaa tttaaaagtg gtcgaaaaag ccgataacgc agcgcaagtc aaagacgcgt
    31981 taacgaagat gcgcgccgca gcgctggatg cgcaaaaagc gacgccgccg aagctcgaag
    32041 gcaaatcacc ggacagcccg gaaatgaaag acttccgcca cggtttcgat attctggtcg
    32101 gtcagattga cgacgcgctg aagctggcaa atgaaggtaa agtaaaagaa gcgcaggctg
    32161 ccgcagagca actgaaaacg acccgcaacg cctatcacca gaagtatcgt taattcctta
    32221 ttgggcggtg aaacgttacc gcccaacgct ttcttctccc ttcgtaaaat caccgcaata
    32281 tctatcttca ccacggcata ttcctgcgtg tcgtcctcat ttttgcgagg cgttttccag
    32341 aaatgaatgt aatgcatatg taatgctata atgcattaca cttttcataa ttaccggagg
    32401 cataatggcc acacttaacg tccgcctgga tgacaaactg aaaaatgagg cttatgcggt
    32461 gctggaaaaa ttaaatatca cgcctactga agcagtgcgg ctgctgtttc agtacgttgc
    32521 ggaaaatgga cgaatgccgg taaaaacgct cactgtcagt gatagtgaag aagccttgtt
    32581 acgcactgtc agggaacgac tggccaaccc acaagaggcc attaaggtcc gactggatga
    32641 actatgagct ggcgtttgat ccgcgggcgc tgaaagagtg gcaaaaactg ggcgtgacag
    32701 tccgggaaca atttaaaaaa aagcttgagg atgtgctgaa aaggccgcgc aatccatcgg
    32761 ctaaactgcg ggatttaccg gactgctaca aaattaagct gcgcacacag ggatatcgcc
    32821 tggtttatca ggttaacgat aaagagttgc tcgtgttggt gatagctatt ggtaagagag
    32881 agaactcagc cgtttatgaa gacgcgacta aacggctgga tgagtgacta acgcaaatga
    32941 tgcaccacct gattgctgct gccgcgccag atcagggaag ggtcttttaa atcctgcaca
    33001 aatttgccgt cgaccagtac gttaatcaaa tcaacaacct gcatttgcgc agcgttgagt
    33061 tcgtcgagtt tatagcctgt ccacacccag atgtctttac ccggacattc ggcgcggatt
    33121 cgttgtacca gtttcagaat atccggcacg ttttgcggat gcagcggatc gccgccggag
    33181 agggaaatcc cctggcgttt gatgcgagtg tcatttagat cgttaatgat ctggtcttcc
    33241 attgctttgg taaatggctg accggaattt acccgccatg tgcttttgtt atagcaaccg
    33301 gggcattcat gaacacaccc ggagacaaac agggtgcagc gagtgccggg gccgttgacg
    33361 atgtcgacag gatagtactg atgaaagttc attttcgatt ctcaattgac gccatgactg
    33421 ctctggtgcg tacgggcgaa gtcccccgat gctacgcatt tgccaccttg cgacgacacc
    33481 aactggccag aggcaattca ttatgtcggg aatcaggaag gggcacggaa cggtaccctc
    33541 tcccttgagg gagagggtta gggtgagggg gaaggattaa cctatctgcc cattccccaa
    33601 atgtttaacg cggcgcttaa cttcttcctg cttaccggcg ttaaatggac gtgcatccgg
    33661 gctacctaaa tatccgcaca cgcggcgagt caccgacaca cgggaggcat catggttacc
    33721 acatttcggg caagtgaagc ctttgctggt gcactcaaac tcgccggtaa agccacactc
    33781 gtagcattca tcgatcggtg tgttggtgcc gtaatacggt acatgttgat agctgtaatc
    33841 ccagacatct tccagcgcct tcaggttgtg ctgaatgttt ggatactcgc catagcaaat
    33901 gaagccaccg ttcgccagtg gcgggtaagg cgcttcaaag tcgatcttgt cgtacgggtt
    33961 caccttcttc tccacatcga ggtggaaact gttggtgtag taacctttat cggtcacgcc
    34021 cggcaccacg ccaaactcag cggtatcgag acggcagaag cggtcgcaca ggttttcact
    34081 cggcgtgctg tagagactga agccataacc cgtttcttct ttccactgat ccactgcctg
    34141 acgcagacgt tcaacaatcg caataccttt cgcgcgcagc tgctcgttgt cgtaaacatg
    34201 ctcgccgccg aacagcgcgt taatggtttc gtggatgccg atgtaaccca gcgaaataga
    34261 cgcacgaccg tttttgaaga tttcagaaac gtcgtcgtca gcattcagac gcacgccaca
    34321 agcgccttcc atatagagga tcggggctac gcgcgctttc acgccttcga gacgagcgat
    34381 acgggtcatc agcgccttac gtgccagcac cagacgttca tccagcagct tccagaaggt
    34441 ggcttcatcg ccttttgcct ccagagcaat acgcggcagg ttcaggctga tcacgccgag
    34501 gttgttacga ccatcgtgga tctgctcgcc gttttcattt tcccacacgc cgaggaagct
    34561 gcggcagccc atcggggttt taaacgaacc ggtgactttc actacctgat cgtagttcag
    34621 gatatccgga tacatgcgct tgcttgcgca ctccagcgcc agctgtttga tgtcgtagtt
    34681 cggatcgcct tttttatggt tcaggccatc gcgaatcgca aacaccagtt tcgggaacac
    34741 cgcagtttta cggtttttac cgaggcctgc gatacgatta cgcaggatag attcctgaat
    34801 caggcgcgat tcccagctgg tgcccagacc aaaaccaaag gtcacaaacg gcgtttgacc
    34861 gttggcggtg tgcagggtgt ttacttcgta ctccagcgac tggaaggcat cgtagcactc
    34921 tttgatggta cgagagttag cgtagccttc ggcgtccggg atgttccact cttctgcggt
    34981 cttgcgatgt ttgttgtagc tggcggtgac aaacggtgcc agcacttcat cgatacggtt
    35041 aatggtggtg ccgccataaa tatggctggc gacctgggca ataatctgcg cagttaccgc
    35101 ggttgccgta gagatcgact tcggcggttc aatctcggcg ttccccattt taaagccctg
    35161 ggtcagcatg cctttcaggt cgatcaacat gcagttgaac atcgggaaga acggcgagta
    35221 atcgagatcg tgatagtgaa tatcaccacg ttcatgtgcc tgcaccacgt cacgcggcag
    35281 caggtgctgg cgtgcatagt gtttagccac gatcccggcc agcaggtcgc gctgggttgg
    35341 aatcaccttg ctgtctttgt tggcgttttc gttgagtaac gaggagttgg tctgctcgac
    35401 cagaccacgg atctcctggt tcaggcgacc gcgtttttca cgttcaatgt cgcgatcgtg
    35461 acggtactcg atgtaagcac gagccagttg tttgtatgga cccgacatca gctggttttc
    35521 aactgcggtc tggatctcat tgatatccac ctggttgcgg ccctgcatct gctcgctgac
    35581 aaccgcggca acagtggcgc aataatcggc atcatcgact tccgctgctt tagctgcacg
    35641 cagaatcgct tctttgatgc gctctgattt aaacggcact ttgcagccgt ctcgtttcat
    35701 cacatgcggt gtcatgatcg ctccatattt ttaagaacag gttatccaca gaaattggga
    35761 aagacgtttt ccggtttttt tgtttcgttt tccggtgctt tccgcaatcg ccatccgctt
    35821 atccacaacg ccgcaccttt ttcgtgggga gctgtagtag caattatagt cgattaatac
    35881 aacatattgg gttgggacgc attttaaagt ctatatatag tgctttgcat caaggatgtt
    35941 tgaacttttt ttgatgtagc tcaaagtaaa aagcagagcg tacggatgac gggcgctaca
    36001 gcgatatgta aattttttaa tgaatttttt gattataaaa tcaacaaaaa caacatactg
    36061 acaggcaaaa ccccggaatg acaggcatta ccggggctta gcgaagattt acttctgtaa
    36121 ccaccagaca gcctcaaaag gccgtaaagt catggcacag ggttgtggtg aggcttcttc
    36181 gtagttatgc atcacaagct gccagttgcc gagcattttc cctggctgcc agggttgggt
    36241 ctcacggcta aggttggcaa tgaccaacaa ggtttgcccc ttccattcac ggcgatagca
    36301 ccacaataca gggctgtttg gcagcagatc ctggtaatcg ccccatgtca gaacggcttc
    36361 ttgcttacgc agtgcgatta acttttggta ggtgtaaaac accgaggact catcggccag
    36421 cgcggcttct acgttgattt gttgatagtt atcgcccagg ccaatccacg gttcgccagc
    36481 cgtaaacccg gcattatcgc cgttgctcca ttgcatgggt gtgcgactgt tgtcacggga
    36541 tttactggcg aggattgcca ataactcgtc ggcatcacgc ccatcgttgc gcagctcggc
    36601 aaacatattg aggctctcca cgtcgcgata gtcagtaatg cgcgtgaaat gtgggttagt
    36661 catgccaatc tcttcgccct ggtagatata tggcgttccc tgcatgccat gcagcaccat
    36721 cgccagcatt tttgccgcag gcacgcggta ttcaccttca tcaccaaagc gagaaacaat
    36781 gcgcggctga tcgtggttac accagaacaa ggcattccat gccacgttgt gcattccttg
    36841 ttgccagtgg cggaacaatg ttttcaacgc cacaaagtca ggtttagcca gcgtccattt
    36901 ttcaccaccg ggataatcga ccttcaggtg atgaaaatta aaggtcatcg acaattcact
    36961 gcctgtcagt gccgcatatc gctggcaatg ctcaaggctg gtggaggaca tttcacctac
    37021 ggtcattaac ccgcgtggcg taaacacatc gcggttcatc tcgtgcaaaa actcgtgtgc
    37081 tcgtggcccg tcggtgtaga agcgacgccc gtcgccatcc aggtcatcag ggaaacgcgg
    37141 gtctttggag atcagattca ccacatccag gcgcaacccg tcgaccccac gatcggccca
    37201 gaactcacag acttttttca gctctgcgcg taccgctgga ttctcccagt tgagatccgc
    37261 ctgttctggt gcaaagagat gcaaatagta ctgttcgctt tccgcatgcc agcgccacgc
    37321 actaccgcca aattttgaac gccagttgtt cggtggtgtt tctggttctc catcgcgcca
    37381 gatataaaac tggcggtaag ggcttgcttt gttcagcgcc tcgcgaaacc aggcatgttg
    37441 ggtagaggta tggttaaaca ccatatcgag aatgatacga atcccgcgcg attttgcctg
    37501 tgtcaccagt tcgtcaaaat cgtccagcgt gccataggtg ggatcaatcg ccgtataatt
    37561 cgctacgtcg taaccgttat cgacctgggg agagacataa aagggggtta gccagatggc
    37621 atcaacgccc agtttatgca gatagtccag gcgctggata acgccacgta aatcgccggt
    37681 gccgctaccc gtggtgtcct gaaaactctt tggataaatc tggtagataa cgccgttttg
    37741 ccaccagtgg ggaagattag tcattacgtt attcctgcaa atgcgagggg gcgcaattgc
    37801 gccccgaaga aaattaaaca atgtccagcg tgcccaggcg gtatttccgc tgatagataa
    37861 acgaggtgag tacaatcggg atgatgatgg cgatagccat tgccagcgca aacacctgcc
    37921 agtagctcgg ttgaatcgag agaattcccg gcaggccgcc tacgccgatg ccattcgcca
    37981 taacgctgtt caggccgcat agcaatcctg ccagaccaga accaatcatc gcgcacagca
    38041 tcgggaagcg atatttcagg ttgatgccgt acatagcagg ctcagtgact ccaagccagg
    38101 cggagatagc ggcaggcacg gagatctcgc gttcattgtg cttgcggctg gaaatgataa
    38161 tgcctatcac ggcggaaccc tgagcgatat tcgacagcgc aatcagcggc cacactggcg
    38221 taccacccat gctttgaatc atctgcaaat caatagcaag cgtggtctga tgtacgccgg
    38281 tgatcaccag cggggcgtac aggaagccaa acaatgccgc gccaatcgga gcaaagctgc
    38341 cggtcatcag gtgacgtacc gcaaaggcaa cgccatcgcc aatcatgcga ccaaacggac
    38401 caatcagcgc atgggcgagg aacaccgcga ggatcagcga acagacgggc accaccacca
    38461 gatagaggta atccggcacg atgcgcttaa gacgagtttc aataacgccc agcgccagcc
    38521 cggctaacag tgccgggatc acctgcgcct gatagcccac tttggcgatg ctgaacatgc
    38581 caaagtccca tacttccggc agctgctgcc cgagcagata agcgttcatc agctgtggag
    38641 aaaccagcgt tacgccaagc acgataccga ggatcggtgt accgcccatt tttttcaccg
    38701 ctgaccagca aatcccgacc ggtaggtaga agaagatcgc ttcaccgatc aaccacagaa
    38761 aatcgtagat cgtttgcagg gaagggtaca tttgcgccag cgtctgaccg ttgctcatgg
    38821 gcaaatcgcc gatcacattg cgaaaaccga ggatcaaacc accactaatc aacgcgggca
    38881 gcaacgggaa gaagatctcc gcgaaatgag agatcaactg ctcatgccat ttcatattct
    38941 gccgtgcggc tttttttacc tgctctttat caacctgcgc ctgtccggtt gacgctatca
    39001 gtgcttgata gtaatcaccc acgttggtgc caattaccac ctgaaattgc ccggcattgg
    39061 tgaaacagcc tttcaccatg gggagttgct caatttcttt cggtctggca ttggccggtt
    39121 ggttgaggac aaagcgcagg cgagttatac agtggctcac tgtcgcaata ttgccgcgcc
    39181 cgccgaccag ttcaatcaac cgatcgatat ccgtttggtt tattttgctc atcataaagc
    39241 cccatggcag atgacatttt tggttggctg cagaatatat tgcgcggaaa aatttaaaaa
    39301 cgggaacgtt cccgaaacgc agcgaagatc acaatttatc gttcaggaaa cgatcaggac
    39361 agggtggcgg ggatgatgat ttgttgcggt tcgctgcgcc cggttacctg cgcgatcaac
    39421 tggcaggccg cctggcgtcc agcttcggcg taaccgggat caacggtgac gatctccgga
    39481 tggaggaatt tcattaacgg tgtattaccg acgctcgcca gttgcaaggt gtcgatgcgt
    39541 tgctcttgca ggtatttact tgcgccaagt gccagcgtgt cggttgcgca cagtaacgcg
    39601 gtagtttcag gcgtaatcac ttttgctacg ttttcatagc cttgcttcat agcaagccct
    39661 ggcagagcgg caacgggatg caatttatgc gctttgcaga acgccaggta ggcttcgtga
    39721 cgtcgcttac cggtcgtcac gtcactatgc ggcacgccga gataactgat attacgatgt
    39781 ccctggtcat acagccgttg catcaggatc ttgattgccc cttcgtcgtc ataacagacc
    39841 gaagcaaagc cttttgcgtc acgcgccagc agaaccagcg atgactgcca gtgggctaac
    39901 atttcttctg ttatgccggt aaaaccgaac agcactacgc cgtcgatatt acgccgtttc
    39961 agcaccccca aatgttcggc aactaattgc ggggaaaact gactttccat catgattggg
    40021 tcgtaacctt gttcatagaa cgctggcagc atggtttgaa cggcgagatt ttctgacaac
    40081 gaatccagac gggtaacaat gatggcgacc actttatcgc tttgaccacg catagcgcgc
    40141 gcagagcggg aaggggaaaa tccatgctga ttcatcactg cttcaacacg ctcgcgcgtg
    40201 cgctggctca cgccgctttc gttattcagc acccgggaaa ccgtagattt ccccacgccg
    40261 cttaagcgcg cgatgtcttt gatggtcagc cgattttgca tcctgttgtc ccgtaacgtg
    40321 ttgtttaatt atttgagcct aactttaccc gtgcattcag caatgggtaa agtctggttt
    40381 atcgttggtt tagttgccag caggtattat atcgccatag atgctacgaa tattattgga
    40441 ttctccttat tatttgcggc gcttttttca cttaccggag gttatatgga acctgatccc
    40501 acgcctctcc ctcgacggag attaaaactt ttccggtaag cccgtctttt cacggcgtta
    40561 ccggatgcgt aaggccgtga cgttttaacg tcccagctca gctttattac cttcaggtaa
    40621 ggcttcgcca cgcctgaaga catttctgta ctgtttcaga cagtgcggag ggactcctta
    40681 tgtttaaaga aatttttacc cggttcattc gccatttacc ttcccgtctg gttcatcgcg
    40741 atccattgcc tggcgcgcag cagacagtga atacggcggt cccgccgtcc ttaagtgcgc
    40801 attgcctgaa aatggcggtg atgcccgaag aagaattgtg gaaaacgttc gacacccatc
    40861 cggagggatt aaatcaggcg gaagtggaat ctgcccgcga acaacatggt gaaaataaat
    40921 tacccgcaca acaaccgtcg ccgtggtggg tacatttatg ggtctgctat cgcaacccct
    40981 ttaatatttt actcaccatt ctcggcgcta tttcttacgc cacggaagat ttatttgccg
    41041 cgggcgttat cgcgctaatg gtcgctattt ctacgttgct gaactttatt caggaagcac
    41101 gttccactaa agcggcagat gccctgaaag cgatggtcag caatactgcg acggtgctgc
    41161 gcgtaattaa cgacaaaggc gaaaatggct ggctggagat cccgatcgac cagctggtgc
    41221 ccggcgatat tataaaactg gcggcagggg atatgatccc ggcagattta cgtatcttgc
    41281 aggcgcggga tctgttcgtc gctcaggcgt cgttaaccgg tgagtctctg cccgtggaaa
    41341 aagccgctac cactcgccag ccggagcaca gcaatccgct ggagtgcgac acgctgtgtt
    41401 ttatgggcac caccgtggtg agcggcacgg cacaagcaat ggtgattgct acaggtgcca
    41461 acacctggtt tggtcaactg gcggggcgtg ttagtgagca ggaaagcgag ccgaatgcct
    41521 ttcagcaagg gatcagccgc gtcagtatgc tgctgattcg ctttatgctg gtgatggctc
    41581 cggtggtgtt gcttatcaac ggttacacca aaggtgactg gtgggaagcg gcactgtttg
    41641 cgctttcggt cgcggtaggc ttaacgccag aaatgttgcc gatgattgtt acctcgacgc
    41701 tggcgcgcgg cgcagtaaag ctgtcgaaac agaaagtgat cgtcaaacat ctggatgcca
    41761 ttcagaactt tggcgcaatg gatattctgt gcactgataa aaccggcacc ctgacacagg
    41821 ataaaattgt gctggagaat cataccgata tctccggtaa aaccagcgaa cgcgtgctgc
    41881 atagcgcgtg gttgaacagt cattaccaga ccggacttaa aaacctgctt gataccgcgg
    41941 tgctcgaagg tacggatgaa gagtcagcgc gctcgctggc cagccgttgg caaaaaattg
    42001 atgagattcc gtttgatttc gagcgtcgtc ggatgtcggt ggtagtggca gaaaataccg
    42061 aacaccatca gctggtttgc aaaggtgcat tgcaggaaat cctcaatgtg tgttcgcagg
    42121 tgcgtcacaa tggcgagatt gtaccgctcg atgacaccat gctgcgtaag attaagcggg
    42181 ttactgatac gctgaatcgt caggggctgc gcgtggttgc ggtggcgacg aaatacctgc
    42241 cagcgcgtga aggagattac cagcgggcgg atgaatccga cctgatcctc gaaggatata
    42301 ttgctttcct tgatccgccc aaagagacaa ctgctccggc actgaaggca ttaaaagcga
    42361 gtggaattac cgtaaaaatc cttactggcg acagtgagtt agttgcagcg aaagtgtgcc
    42421 atgaagtggg gctggacgcg ggagaggtgg tcattggtag tgatattgaa acgctatctg
    42481 acgacgaact ggcaaatctc gcacagcgca ccacgctgtt tgctcgcctg acgccgatgc
    42541 ataaagaacg cattgtgacc ttactgaagc gcgaagggca tgtggttggc tttatgggcg
    42601 atggtattaa tgatgcgccc gccttacgtg ctgcggatat cggcatttct gtggacggcg
    42661 cggtagatat tgcccgtgaa gcggctgata tcatcctgct ggaaaaaagc ctgatggtgc
    42721 tggaagaggg ggttattgag ggacgtcgca ctttcgccaa catgctgaaa tacatcaaaa
    42781 tgacggcgag ttctaacttc ggtaatgtgt tcagcgtgct ggtggcgagt gctttcttgc
    42841 ccttcctgcc gatgttgccg ttacacttgc ttattcagaa cctgctgtac gatgtgtcac
    42901 aggtggcgat cccgtttgat aacgtcgacg acgagcaaat tcaaaagccg cagcgttgga
    42961 atccggcgga tctggggcgc tttatgatct tcttcggacc gatcagctcg atcttcgata
    43021 ttctgacgtt ttgcctgatg tggtgggtat tccatgccaa cacgccggaa acgcaaacgc
    43081 tgttccagtc gggctggttt gtggtgggct tactgtcgca aacgctgatt gtgcatatga
    43141 tccgcacccg tcgtgtaccg tttattcaga gctgtgcatc gtggtcgtta atgatcatga
    43201 ccgtgatcgt gatgattgtc gggatcgcat tgccgttttc accgctggcc agttatctgc
    43261 agctgcaggc gctgccgtta agctatttcc cgtggctggt tgcgattctg gcagggtata
    43321 tgacgttaac ccagttggtg aaagggttct atagccgtcg ttacggctgg cagtaaagaa
    43381 taaactgggc atggtggccc ggattcagat tgcagacaaa gtgagctttg ttcatgccgg
    43441 atgcggcgta aacgcccaat ccggcctaca aattcaataa attgcaggaa tcacgtaggc
    43501 ctgataagcg tagcgcatca ggcgattttg cgtttgtcat cagacttaat ccgggcatga
    43561 tagcccggat ttccatcaag attagcgacg aacagcgatc gcttcgatct caatcttcac
    43621 gtctttcggc agacgggcaa cttcaacgca agaacgtgcc gggaaggtgg cgttgtgttc
    43681 ggtgaagaag gcttcgtagg tagcattaac ggttgcgaag tcgttcagat ctttcacaaa
    43741 cacggtggtt ttaacgatgt cgcccacttt caggcctgcg gcttcgacga tcgcttttac
    43801 gttatccagc gactgacgag cctgtgcagc gacgtctgcc ggtacttcgc ccgttttcgg
    43861 atttaccggg atctgaccgg aggtgatgat catattgccc agatcaacgc cctgtacgta
    43921 aggaccgata gctgccggtg cattttccgt cgcgatagtt ttgctcatga tttctccttt
    43981 attacagcgg taaaaagtct ggctcattat agggagccag acttttatta ccaaccgcaa
    44041 ttaattggcc agcaccacat tatgggaaaa ctctttttca cagtatttgc atttgagcgc
    44101 gatgtcattg gcgcgttttc gcacggcaaa gctggaagaa accggttcgg catggctgat
    44161 acagttgctg ttcgggcaga ccagcacatt gtcgatgcgc tccggcagac tggggcgcga
    44221 tttgccaacc acttcatagt tgtcgatacg gttaaccgtg gcttgcggtg catacaatgc
    44281 cagttggtct acttgatctt cactcaaaaa ggtattttcg attttgatca gatctttgcg
    44341 gcccatctcg ccagaaggca ggttcagacc aatggtgatg cgctgatccg tttcggtcag
    44401 cttgaacaaa ctcaacagct taaaaccgat ctgggcgggg atatggtcaa ttaccgtgcc
    44461 gcgtttaata gcttcaacct gcaatttatt atcgtgtgtc atctctattc ccccttacag
    44521 taccagatcg cgattcagaa ccagtgccag taacgcctgg cgagcgaaaa tcccgttgcc
    44581 tgcctgctgg aagtaccagg cgtgtggcgt tttatcaaca tccgtcgcaa tctcatcaac
    44641 acgcggcagc ggatgcagca ctttcatatt ggctttggcg ttgtggagat cgctggcgcg
    44701 aagaacaaac tgcgctttca cgttggcgta ctcggacggg tccagacgct ctttttgcac
    44761 gcgggtcatg tacaggatgt ctacttccgc catcacttct tcaatagagc tgtgcagact
    44821 ccaggcgatc cctttttcat caagcatatc cagaatgtat tgcggcattg ccagcgcgtc
    44881 cggcgcgatg aagtaaaaac ggttgccgtc gaacttcgct aacgcctgag tcagggagtg
    44941 aacggtgcgg ccatatttca ggtcaccaac cattgcgacg tggagattgt ccagacgccc
    45001 ctgggtttcc tgaatggtga ataagtccag caaggtttgc gtcggatgtt ggttggagcc
    45061 atcaccggca ttcagtaccg gtacattgcc agaaaattcg gtggccaggc gcgccgcacc
    45121 ttcctgcgga tgacgcatca ctatcgcatc gacgtaagtg ctgataaccg aaatggtatc
    45181 ggccagcgtt tcgccctttt tacccagtga tgtattggcg ctgtcggaga agcccaccac
    45241 gctggcaccc aggcggtgca tggaagtttc gaaagagagg cgggtacggg tagaggcttc
    45301 gaagaaacag ctggcaatga ctttgtgctt caacagctct ggttgcgggt ttgctttcag
    45361 tttcgccgct gtcgccagca ccagattaag gtcatcgcga ctaaggtcgt ttatggaaat
    45421 gatatgtttc tgatatagcg gattagccat cttttatctc ctgacgcctg ggcaaaaaaa
    45481 aagcccctcg attgaggggc tgggaatggg tgatcaacgg gaagaaaaac ggcaggccag
    45541 cgtctttttt cagacgcggt aagacaaaat gtcgaacaca ctgaaccata catcctcccg
    45601 gcaaattgtc cggcattata ctcatcgtca gaagcggcgc aagcatttga tgcaatattt
    45661 tgtcagcgca aacggtttat ttgaattaaa agtcaaggta tatgcatttt tatttgatgt
    45721 gattctgcag gggaactgtc cttcggtatc aataattgca gacattccct gctttttcct
    45781 ttttcacttt cacgcaatca gatatgcatt ttattccatt catccgactt atagggcgga
    45841 gtgtgaaagc gaacggctaa cactcttgct tactgctcag ggatgcgcgc tatcacttta
    45901 atttcaaaat caaagcctgc cagccatgta acacccaccg ccgtccagtt tggataaggt
    45961 ggggcgctaa atatttcatt tttcaccgtc atgatgtctt caaattggtt ttctggatcg
    46021 gtatggaagc tcgtaacatc aatgatatcg tcaaaagtgc atcccgcagc agccagggtc
    46081 gcatgcaaat tatcaaatgc cagtctgact tgttgctgaa aatcgggttc tggtgttccg
    46141 tcctctcgac ttcctacttg cccggaaaca aacagcaaat cgccggaacg aatagccgca
    46201 gaataacgat gctcagcata cagtgaatgt cggccagcag ggaaaacagc ggttctttct
    46261 accatttggt tatcctcaag atttacgaca tgaacagaag atttctcttt accgggagcc
    46321 gcttttagcg gacgacgtga gtaaacaaaa cccagacatc atggataatg gctgggctta
    46381 attgagcgta gtcggttatg cgccaaacgc gccatcaatg gtatgcatcg cgccggtaac
    46441 aaaactggct tctggccctg ctaaccatgc gaccatacca gcgacctctt ccggttgccc
    46501 atgtcttttg atagccatca aactatgcaa catatcgcgc attggcccgt tggcgggatt
    46561 agcgtcggta tcaattggcc ctggctggac gacgttaatg gtgatcccac gcggtccaaa
    46621 atcacgggcc agcccgcgcg ccatgccttg cagggcagat ttgctggcgg cataagcagc
    46681 catgcctgca acaggcatac gatcgccatt cacggagccg atgattaaga tgcgcccgcc
    46741 ttcgggcatc tgccgggcgg cttcaacaga ggcatgataa ggagcatgaa tattgatttt
    46801 gaaaaggcga tcaatatcgt cggcatttaa ttccagggca tcgccaaaga cgccaatacc
    46861 tgcatttacc accaggatat ccaatgcgcc gctcttacga acgacatcaa tgacagcgtc
    46921 tctgtcagca ctatctgtaa atactgctgt cgctccagtc tcttgtgcca gatgttcagc
    46981 ggcatctttc gaccccgcat aggtgaatcg tacattggcc ccatcagtga cgaaacgacg
    47041 tacgatagcg gcaccgattc cacgactgcc accgaggatg agaactgtct tacctgtaaa
    47101 agcgcccata aggactcctt gatttattat gtaacatgca ttacaaaact gttttaactt
    47161 tctgtcaaca agttttgtaa tggtcactaa aaaacaatct cgcgttccag gtcgtcccag
    47221 acgtttcgct cctgagcagg cagtctctgc ggcaaaagtg ctttttcacc aaaaaggttt
    47281 cgatgctgtc agtgttgctg aagttactga ttatcttggt attaaccccc cgagcctcta
    47341 cgcagctttt ggcagtaaag ttgggttatt tagccgtgta ctcaatgaat atgtcggtac
    47401 ggaagctatt ccgcttgccg atattcttcg tgatgatcgt ccggtaggcg agtgcctggt
    47461 tgaggtatta aaagaagcgg cgcgcagata tagccaaaac ggcggctgcg ctggctgtat
    47521 ggttcttgaa ggtattcata gtcatgatcc acaagcgcgt gatattgccg ttcaatatta
    47581 tcacgccgca gaaacgacca tttatgacta tatcgccagg cggcatccac aaagcgcaca
    47641 atgtgtgact gattttatga gtaccgtgat gtcagggctt tctgcgaagg cacgggaggg
    47701 gcactccatc gaacaactct gtgcaacagc tgcactggcg ggggaagcga taaaaactat
    47761 tctcaaggag tgatgctggc cttgacccga aaggcgggaa cgcgcctgcc gataagttgt
    47821 gataagacaa taattcacgc gttaaggcta gcggaattga tcatcttttc gtataacgat
    47881 agaaatgaaa cattgtttta attaaagagt ggaaaagatg atcattggaa atattcataa
    47941 tcttcagccg tggctacccc aggagttacg ccaggcgatt gagcatatca aagcgcacgt
    48001 tacgccagaa acgccaaagg gtaagcacga tatcgaaggc aatcgtctgt tttatcttat
    48061 ctcggaagat atgaccgagc cgtacgaagc tcgccgtgcg gagtaccatg cccgctatct
    48121 cgacattcag attgtgttaa gaggtcagga aggcatgacc ttcagcacgc aacctgcagg
    48181 cacgccggat accgactggt tagctgataa agacatcgca tttttgccgg aaggcgttga
    48241 tgagaaaaca gttatcctaa atgaaggtga ttttgttgtg ttttatccgg gggaagtgca
    48301 taaaccgctg tgcgcagtgg gcgcaccagc ccaggttcgc aaagcagtag tgaagatgct
    48361 gatggcgtga tgacttttcg ccgtaaataa caccaggttt acggcgagtt tgtgaaaaga
    48421 gcgttttttg atattttttt gtgagtaaaa tttgtaatgc ttagacgttc ttattaactc
    48481 aaggagtccg tcatgagcaa aatatcagat ttgaattatt ctcaacacat tacattagcc
    48541 gacaatttta aacaaaaaag tgaagtttta aatacctggc gtgttggaat gaataatttt
    48601 gctcgtaatg ccgaggggca ggataataca agaaatatcc tcgatcctaa gacattttta
    48661 gagtttttgg taaaaatatt taccctgggt tatgtggatt ttagcaaccg ctccaacgaa
    48721 gcgggaagaa atatgatggc tcatattgag tcctcatctt atatcaaaaa taatgatggc
    48781 agtgagataa tgaagtttgt tatgaataat cctgaagggg agcgagcgga tttatctaag
    48841 gtggaaatag aaattacact ttccactatc actactatgg ggactcgtca aggacatacg
    48901 gctattatat ttcaacaacc tgatggttcg actaatcgtt atgaaggaaa gtcctttgaa
    48961 agaaaagatg agagttcatt acacctgatt actaacaagg ttctggcgtg ttatcaacgt
    49021 gaagctaaca aggaagtggc gcttctatta aataatcatc agttgaactg gaaggacgca
    49081 attgattata acgagttatt agaaaagcca ttagccagta cattagaaaa aataaagaaa
    49141 gaacatctgc tattaatgcc tcatgtatgt gatgacacga ttccatactt actaggcgaa
    49201 ggtggtatac ttgaagaaat aaatggtctg aaaacgttac atgataccaa aattgatagt
    49261 ggtacagaag gtaatgatga aattaataac atcaaaatta acctgtctaa tattcttatt
    49321 gattccttag atgatgcaaa aattaatctc gaaaaggtca tcgattcgat gttggagact
    49381 ttttttaaat tgccatatat taatgatgta aaaattctgg agtggtgttt taatcaaagt
    49441 atgcaatatt ctgatgatac tacaaagata aagcatgccc gctccgtgat cgatcatatt
    49501 gattttaact gcgatcagtc taaaatagct gagacattat ttttcaacct caataaagaa
    49561 ccctataaaa acagccctga gttacaggaa ttgatttggg agaagttggt tgtatatgtc
    49621 aatgatttta atttaagtaa tcaagaaaaa tcgcgcttaa tactaaggct ttttgatgat
    49681 gttaaattgg tttttgataa agtgcctctc agcattttag tgaatgatat ttttttgaag
    49741 gattttttca tgaagcaacc tgattttgct aagtggtact tctatcaatt acttaagagt
    49801 tatgagggcg agcagatgta tcttaatgag ttaggatatg tttatggtga tgaagaaaaa
    49861 acaaaagaaa ttgtaaataa acttccgggt tatgtcgtta agatttttga agaaaagatc
    49921 gataatgaat taaaaattag aacaagaatg atggaaatcc tccgggatga gcaaatcaat
    49981 atatatgaat atattaatga aaaacagtta gaaaaattaa atcctcaggg aaatttacgt
    50041 atagcaatag aaaaatttgg gtggaataat aaacctatca ctgcataaaa taatgtgatg
    50101 ccgggatggt ttgtatttcc cggcatcttt atagcaatat caattattta ctgagcgtcg
    50161 cgaccatcac cgctttgatg gtatgcatgc ggttttccgc ctgatcaaaa acaatgctgg
    50221 cggcagattc gaagacctca tcagtcactt ccattccgcc atgtaggcca aactcttccg
    50281 ccattttctt accaagcgtc gtctggtcgt catgaaaagc gggcaggcag tggaggaatt
    50341 tgacctccgg gttaccggtc aactgcatca tcttgctgtt cacctgataa tcacgcagta
    50401 atgcaatccg ttctgcccat ttctcttttg cttcccccat cgacacccac acatcggtat
    50461 agataaagtc agcaccttct actcccttgg ctacatcttc cgttagcgta atattcccgc
    50521 cattttgctg tgccagagcg cggcattccg taaccagcgc agcttccggc cagcatgcct
    50581 gtggcgcgac cagacgcaaa tccagaccgg taagcgccgc agcttcgagc atcgaattgc
    50641 ccatgttgtt acgtgcgtca cctgcataga ccagcgtcat ttcgttgaac gctttgccag
    50701 gtaaatgctc ctgcatggtg aggagatccg ccagcagctg cgtggggtga aactcattgg
    50761 tcaggccgtt ccataccggc acgccagcgt attccgccag tgtttcaaca atctcctgac
    50821 catagccgcg atactgaata ccgtcataca tgcgaccaag cacgcgggca gtgtctttaa
    50881 tcgactcttt atgaccaatc tggctgccgc ttgggccgag ataagtaacg cgagcaccct
    50941 ggtcatatgc ggcaacttcg aaagagcatc gggtacgggt cgagtctttt tcgaagatga
    51001 gcgcgatgtt tttaccagtg agtttggctt cttctttacc gcttttctta tcggctttca
    51061 gcttcgcggc taactgcaac aggctgttga gttcagctgg cgtgaaatcg agtaatttca
    51121 ggaaatgctt atgataaaac ccggacatag atccctcctg tggctatcgc ctcaatgaat
    51181 taaaattcaa tttatatgga tgattattca tttgcaagtc taaagcataa atctttgtca
    51241 caaaggtgga ggcaatgtca gtggtgtgtg acaataagag tatcggcagg acattaagag
    51301 gaatgagcca tggcaaaccc ggaacaactg gaagaacagc gtgaagaaac acgtttgatt
    51361 attgaagaat tactggaaga tggcagcgat ccggacgcgc tgtacaccat cgaacaccat
    51421 ctttccgcag acgatctgga aaccctggaa aaagcagcag ttgaagcgtt taaactcggt
    51481 tacgaagtga ccgatccaga agagctggaa gttgaagatg gtgatatcgt gatttgctgc
    51541 gacatcctca gcgagtgcgc gttgaatgcc gatctgatcg atgcccaggt tgaacaactg
    51601 atgacgctgg cggagaaatt tgacgttgag tacgatggtt ggggcactta ctttgaagat
    51661 cctaacggcg aagacggcga cgatgaagat tttgtcgacg aagacgatga cgggggtcgc
    51721 cactaattaa ttgattaaaa tgcccgatag cacacactag cgggcatttt tttagagttc
    51781 gcgtagcatc cgcacttcac aatcgacatg gcccgtgcag ccaagcgcat agtcgatatg
    51841 ctcaaagccc aaatgctcat aaagtgcaat ggcttccttt aaaaaagcgg tcgtttccag
    51901 ataacagcgt ttgaagccca tctctcgtgc ctgctccatc gccattaagg ccagtttttt
    51961 cgccagccct ttaccgcgaa tagccggaag aaaatacatc ttttgcagtt cgcaaatgtc
    52021 cgattcacta cctgctaatg gcgcaatccc accgccgccg accacttcac catcgaactc
    52081 aaccacccaa tatgcatggc caggctgact atacacttga tacaactcgt caaggttcgg
    52141 atcggcgacg gtgtagcctt tatcagcggt aagaccgtat tcggcggata cctgacgaat
    52201 gacgcgggcg atagcgggat tatcctgctg cgtcaggcga cgcattacag gtgattgcgg
    52261 cgcaatgtta ttcatagttt aacccaataa aaataaagta attatacatt tgtttaatac
    52321 cactctcttc ttacgtgcgc aagcgactta taaagtaata aagtttttgc cagcctatta
    52381 ctgataagag tattgtaacg tggcagaaca ttttactgtc tgtatatata aattatatca
    52441 atggattttt taattcaggg aatttttatg gctcaagtta ttaatgaaat ggatgttccg
    52501 tcccattcgt ttgtttttca tggcacaggt gagagatatt ttcttatttg tgtggtgaat
    52561 gtgttgttaa cgattataac gctaggtatc tatttgccat gggcattaat gaaatgtaag
    52621 cgttatctct atgctaatat ggaagttaac ggacaacgat tttcttatgg aattaccggt
    52681 gggaatgttt ttgttagttg tcttgttttt gtttttttct atttcgcaat cttaatgaca
    52741 gtgtcagcag atatgccgct tgttggctgt gttttgactt tgtcactgtt ggttttgctt
    52801 atatttatgg cagcaaaagg actgcgttat caggccttga tgaccagtct caacggcgta
    52861 agatttagtt ttaattgctc tttgaaaagg ttctggtggg taaccttttt cttgccgatt
    52921 ttaatggcca ttgggatggg agctgttttc tttatctcga caaagatgct acatgccaat
    52981 agttcaagta gtgttattat atctgtggtt ctgatggcaa tagttggtat tgtttccatt
    53041 ggtattttta atggtacttt atatagcctg gtaatgagtt ttctctggag caataccagt
    53101 ttcggtatac atcgtttcaa ggtgaaatta gatactacgt attgtataaa atatgccatt
    53161 ctcgcatttt tagctttatt accttttctc gctgttgctg gttatattat cttcgatcaa
    53221 atattaaatg cgtatgatag ttctgtgtat gcaaatgatg atattgagaa tttacagcaa
    53281 tttatggaaa tgcaacgtaa aatgataatc gcgcagttaa tctattattt tgggattgct
    53341 gttagcacca gttatttaac ggtgtcgttg cgaaatcatt ttatgagcaa cctgtcactg
    53401 aatgatgggc gtattcgttt tcgctcaact ttaacgtacc acggtatgct ttatcgcatg
    53461 tgtgcgttgg tggtgatatc cgggattacg ggcggtctgg cttatccact gctgaaaata
    53521 tggatgattg actggcaggc aaaaaatacg tatttgctgg gcgatttgga tgaccttcct
    53581 ttaatcaata aagaagaaca accagataaa ggcttcttag ccaggatttc acggggaatt
    53641 atgccttctt taccatttct gtaagagata aaaaaggccg gagcatgcta cggccttcgt
    53701 tttcatcgct gtgatttgat tacagcgcgg cgataacagc ctgctgttca atcagcttcg
    53761 ctttcgcttc tgcatagcct tccagctttt cacgctcttt cgcgatgacc gcttccggtg
    53821 cgcgggcgac aaagccttca ttcgccagct tattctcgat gcggctgatt tcgccttcga
    53881 tcttcgccac ttctttcgcc agacgcgcca gctcatcttc tttgttgatg aggccagcca
    53941 tcgggatcag cagctctgcg ccgtcgatga tcttcgtaac ggaaaccgga cctttgttat
    54001 cggcaggcag cacggtgatg ctttccagac gcgccagggt ttgcaggaag ccacggtttt
    54061 catttacgcg acgttctgca tccgcgctgc aaccacgcag cagcagctcc agcggtttgc
    54121 ccggtgcgat gttcatttct gcacggatgt tacgtaccgc aacgatcgcc tgcttcaacc
    54181 actctgtgtc ggccagtgcg gcttcatcaa cctgagatgc atcgtactgc gggaacggct
    54241 gcagcatgat ggtatcggca gtgataccgc aaagtacttt cacacgctgc cagatggttt
    54301 cggtgatgaa cggaatgatc ggatgcgcga ggcgcagcag accttccagc acggtcacca
    54361 gcgtatggcg agtaccgcgc agttcggctt cggtgccacc gttcattacc ggcttagtca
    54421 gctccagata ccagtcacag aactggttcc aggtgaactc atacagaatg cctgcggcga
    54481 tatcgaagcg gaagctgtcc agcgcttcgc ggtacgcttt gatggtctgg ttgaactctg
    54541 ccagaatcca gcggtccgcc agcgacagcg tcatttcacc gccgttgaag ccgcaatcct
    54601 gaccttctgt gttcatcagc acaaagcggc tggcgttcca cagcttgtta cagaagttac
    54661 ggtaaccttc cagacgcttc atatcccagt tgatgtcacg accagtagaa gccagcgccg
    54721 ccagggtgaa gcgcagcgca tcagtaccgt gcggctcaat gccgttcggg aactgcttct
    54781 cggtgcgctt acggattttg tccgccagtt gcggctgcat catattgccg gtacgttttt
    54841 ccagcagttc tggcagcgaa atgccgtcaa ccatatccag cgggtcgata acgttaccct
    54901 tggatttgga catcttctgg ccttcgtcat cacgaatcag gccggtcatg taaacggtgt
    54961 ggaacggcac ctgcggtttg ccattttcat ctttgatgaa gtgcatggtc atcatgatca
    55021 tgcgggcaat ccagaagaag atgatgtcga aaccagatac catcacgctg gttgggtgga
    55081 actgacgcag ggcgtcggtg ttttccggcc agcctagggt agagaaggtc cacagcgcag
    55141 aagagaacca ggtgtcgaga acgtcttcgt cctgacgcag ggcaacgtcg gcaccgaggt
    55201 tattttcttt acgcacttcg tcttcgttgc ggccaacata aacgttaccc gcttcgtcgt
    55261 accatgccgg gattcggtga ccccaccaca gctgacgaga gatacaccag tcctgaatat
    55321 cgcgcatcca ggagaagtac atgttttcgt actgcttcgg tacgaactga atgtcgccat
    55381 tctcaaccgc ttcaactgcc ggtttcgcca ggacatcggc acgcacgtac cactggtcgg
    55441 tcagcatcgg ttcgataact acgccgccac ggtcgccgta aggaacggtc aggtcgtgcg
    55501 gtttaatttc ttccagcagg ccgagtgcgt caaccgcagc aacgactgct ttacgtgcag
    55561 caaaacgctc gagtttctgg aactctgccg ggatttcgct ggaataaacg tcagattcgt
    55621 tacctttggt atcgaacacc tgggcgcttt cacggatatc gccgtcaaag gtcaggatgt
    55681 tgatcatcgg cagcgcgtga cgtttaccca cttcatagtc gttaaagtcg tgcgccggag
    55741 tgattttcac gcagccggtg cctttttcca tgtcggcgtg ttcgtcgcca acgatcggaa
    55801 tacgacggtt aaccagcggc agaatgacat atttgccaat cagatctttg taacgcggat
    55861 cttccgggtt aacggctacg ccagtatcgc ccagcagggt ttccggacgg gtagtcgcaa
    55921 ccaccagata atctttaccg tctgcggttt tcgcgccgtc agccagcgga tagcggatgt
    55981 gccacatcga accttttgat tcgcggtttt ccacttccag gtcagagata gcggtgcgca
    56041 gtttcgggtc ccagtttacc aggcgtttgc cacggtaaat caggtcttct ttatacagac
    56101 gaacgaaaac ttctttcacc gcattggaca ggccttcgtc catggtgaag cgttcacgct
    56161 cccagtcgac ggagttgccg agacggcgca tctgacgggt aatggtgccg ccggactccg
    56221 ctttccattc ccagattttg tcgatgaaag cttcgcggcc gtagtcgtga cgggttttac
    56281 cttcttctgc ggcaatcttg cgctcaacga ccatctgggt agcgatcccg gcgtggtcag
    56341 taccgacctg ccacagggtg tttttgccct gcatgcgctg atagcggatc atggtatcca
    56401 tgatggtttg ctggaaggcg tgacccatat gcaaactgcc ggtgacgttc ggcggcggga
    56461 tcatgatgca gaaactttcc tggctttcat cgccattagg cttaaagtag ccctgctttt
    56521 cccagtgctc gtaaagcggc tgttcgatat cttgtgggtt atatgtcttt tccattattt
    56581 ccaggttgcc gtattcaggt tgaaaccagc cacgcggtag gctttatagc gttcgcgcgc
    56641 cagttgtttc agagaatctt cataaggaac gaagtctacc acttctgtga aagcggtggc
    56701 aaaatctgca aagcttgttc gcagactaat caatatatcg cgcgggctgc tgctgcgttt
    56761 ttgcggccag gcgatctcca ccggcgcacc gccgcgcggt ccttctcccg ctaaattatg
    56821 cggaacaaag ctttctgccg gacgcgccca cagggcttca tccagccggt aagcctgctt
    56881 ttcatcttca caggcgatga gcacgcgctt accgctgcgc caacgttctg cggcaatttc
    56941 acacaccagt tgctcaacgg cgcttaagcc atcgacggtg gtgtcattgt ccagaaggta
    57001 gaacgtcgcg tttttcatat atggggcttc ttgtggtgga tttaaatacg catcaggtat
    57061 acaccgcagc gcctgatgcg acgctgttgt gtcttatcag gcctacgagt tcagtgctgt
    57121 gtgtaggtcg gataaggcgc ttacgccgca tccgacaata acagcattgc ctgatgcgac
    57181 gctgccgcgt cttatcaggc ctacgagttc ggtgctgtgt aggtcggata aggcgttcat
    57241 gccgcatccg acaataacag cattgcctga tgcgacgctt gcgcgtctta tcaggcctac
    57301 gagttcagtg ctgtgtaggt cggataaggc gttcacgccg catccggcaa taacagcctt
    57361 gcctgacgca attactcttc gccgttaaac ccagcgcggt tcaacaggaa ctgtgccagc
    57421 aacgctaccg gacgaccggt tgcgcctttt gctttaccag aacgccaggc ggttccggcg
    57481 atatccaggt gcgcccagtt gtacttacgg gtaaagcgcg acaggaagca acctgcggta
    57541 atcgccccac caggacgacc gccaatgttc gccatatcgg caaaattgga ctccagctgt
    57601 tcctgatact cgtcacccag cggtaagcgc catgcgcggt caccggattg ttcagacgcg
    57661 gcaatcagtt catgggccag cggattatgg ttcgccatca gaccggtaat gtgatgaccc
    57721 agcgcgatca cgcaggcacc ggtcagcgtc gccacgtcaa tcaccgcttc cggctcaaaa
    57781 cgctcaacgt aagttaacac gtcgcacagt accaggcggc cttcagcgtc agtattcagc
    57841 acttcaacgg tttgaccgga catggtggtt aacacatcgc ccggacgata ggctcgtccg
    57901 ccaggcatgt tttcgcagcc tgccaacacg ccgataacgt taatcggcag ttgtagctcc
    57961 gcgaccatac gcatcacacc gtaaaccgct gccgcaccgc acatatcgta cttcatctca
    58021 tccatgcctt ctgaaggctt gatcgagata ccgccggagt cgaaggttaa acctttaccc
    58081 accagcacga ttgggcgtgc atcttccgac gcgttgcctt tgtactcaat caccgacatc
    58141 agcgattcgt tctgcgaacc ctgaccgacc gccagatagg aatgcatccc cagctctttc
    58201 atctgctgtt cgccgataac gcgggtgata acattcttgc tgtagctgtc agccagctgg
    58261 cgcgcttgtg aagcgaggta agcggcgtta cagatattcg gcggcatatt gccaagatcc
    58321 tttgctgctt taatcccggc ggcaatcgcc agaccgtgct ggatcgcgcg ctcaccgctg
    58381 gtcagttcac ggcgggtcgg cacgttgaac accattttac gcagcggacg acgcggttcg
    58441 ctcttgttcg ttttcagctg atcgaaactg tagagcgttt cttttgccgt ctcgacagcc
    58501 tgacgcactt tccagtagtt gttacggcct ttaacgtgca gttcagtcag aaagcagacc
    58561 gcttccattg agccagtatc attcagcgta ttaatggttt tctgaataac ctgcttgtac
    58621 tgacgctcat ccagctcacg ttctttgccg caaccaataa ggagaattcg ctcggaaagt
    58681 acattcggaa catggtgcag caacaatgtc tgccccggtt ttccttccag ttcgccccga
    58741 cgtagcaggg cgctgatgta cccatcgctg attttatcga gctgttctgc aatcggagaa
    58801 aggcgacgtg gttcgaagac gcccacgacg atgcaggcac tccgctgttt ctccgggcta
    58861 ccgcttttta cactaaactc catgcactac gctcctgaat cttaaagaca acggcggtgg
    58921 ctacagatag aattgcaagc tttcgtaact catgtccgct gttgcgatga cttcgtgtta
    58981 atcttaacgt tattacggca ttggcacgtc agaacaaaat ctgagaggtg aatccgttga
    59041 gtataattat cttagcgacg atttcgacga ctcaagagaa taaatgacgt ttaagccatg
    59101 aaacaagcta aaatcctgca aaagacgagt ttttacgggc gtatttaaag tgataatcat
    59161 aagatatctg gtgcgggaga cgctcaaaag ccagctggcg atactcttca tcttgctttt
    59221 gatcttcttc tgtcaaaagt tagtgaggat cctcggcgca gcggttgacg gcgatattcc
    59281 ggcgaatctg gtgctctccc ttctcgggtt gggcgtgccg gaaatggcgc agcttatcct
    59341 gccattaagc ctgttcctcg ggctgctgat gacgctgggc aaactgtata ccgaaagtga
    59401 aattacggta atgcatgcct gcggcctgag caaagcggtt ctggtgaaag cggcaatgat
    59461 ccttgcggta ttcacggcaa tcgtagcggc ggttaacgtg atgtgggcgg gaccgtggtc
    59521 atcgcgtcat caggatgaag tgttagcaga agcgaaagcg aaccctggca tggcggcgct
    59581 ggcgcaaggg caattccagc aagcgactaa tggcagctcg gtgctgttca tcgaaagcgt
    59641 tgacggcagc gatttcaaag atgtgttcct cgcgcaaatt cgaccaaaag gtaatgcacg
    59701 tccttctgtg gtggtggccg attccggaca tttaacccag ctgcgcgacg gctcccaggt
    59761 cgtcactctc aaccagggaa cgcgcttcga aggcactgca ttgttacgtg atttccgcat
    59821 tacggacttc caggattatc aggcgatcat tggtcaccag gcggtggcgc tcgacccgaa
    59881 cgataccgac cagatggaca tgcgcacatt gtggaacact gacaccgatc gtgctcgcgc
    59941 agaactgaac tggcgtatca cgttggtatt caccgtgttt atgatggcac ttatggtcgt
    60001 accgctgagc gtggttaacc cacgtcaggg acgcgtactg tcgatgctgc cagccatgct
    60061 gctgtatcta cttttcttcc tgatccagac ctccctgaaa tcgaacggcg gtaaaggtaa
    60121 gctggacccg acgctgtgga tgtggaccgt taacctgatt tatctggctt tagcgattgt
    60181 tctcaacctt tgggacaccg tgccggtccg ccgcctgcgc gccagttttt cgcgtaaagg
    60241 agcggtgtga tgcaaccttt tggcgtactt gaccgctata tcggtaaaac tattttcacc
    60301 accatcatga tgacactgtt catgctggtg tcgctgtcgg gcattatcaa gtttgtcgat
    60361 cagctgaaaa aagccgggca ggggagttac gacgcgttag gcgcaggaat gtataccttg
    60421 ctgagcgtgc cgaaagatgt gcagatcttc ttcccgatgg cggctctgct tggggcgttg
    60481 cttggtcttg ggatgctggc gcagcgcagc gaactggtgg tgatgcaggc ttctggtttt
    60541 acccgtatgc aggtggcgct gtcggtgatg aaaaccgcca ttccgctggt cttgctgacg
    60601 atggcgattg gcgaatgggt cgcgccgcag ggcgagcaga tggcgcgtaa ctaccgtgcg
    60661 caggcgatgt acggcggctc gttgctctct acccagcaag gcttatgggc gaaagatggc
    60721 aacaacttcg tctacattga gcgggttaaa ggtgacgaag agttaggtgg catcagcatt
    60781 tatgccttta acgagaatcg tcgtctgcaa tccgtacgct atgccgctac tgcgaagttt
    60841 gacccggaac ataaagtctg gcgtctgtcg caggttgatg aatctgatct gaccaatccg
    60901 aaacagatta ccggttcgca gacggtgagc ggcacctgga aaaccaacct cacgccggac
    60961 aaactgggcg tggtggcgct ggacccggat gcactctcta tcagcggttt gcacaactat
    61021 gtgaagtatc tgaagtcgag cggtcaggat gccggacgtt atcagctcaa catgtggagc
    61081 aaaatcttcc agccgctatc tgtggcggtg atgatgctga tggcgctgtc gttcatcttt
    61141 ggcccactgc gtagcgtacc gatgggcgtg cgtgtggtca ccggtatcag tttcggtttt
    61201 gtcttctacg tactggacca gatcttcggc ccgctgacgt tggtttatgg catcccgccg
    61261 atcatcggcg cactgttgcc aagcgccagc ttcttcttaa tcagcctgtg gctgttaatg
    61321 agaaaatcgt aaccgaatga acgaataaag ggagcgaaag ctccctttat tattgttagc
    61381 aaagtgtgct tcgttcattc ctgaaaaata attaaaattc aatatattgc agtggggtgg
    61441 gcatgataag acgcgccagc gtcgcatcag gcaaagacaa atctcgcctg acttaccttc
    61501 ttctcccccc cagcaaactc cccaacatcc cgcgtacaat ctgattcgtc acttggcgag
    61561 cggcgctttt ggccattgtt tgcaccacac catctttctt cccgccgcgt ggtccggtag
    61621 tgccgaacaa aatatccttc aatccaccaa gaatgccgtc atccaccgct acctctttcc
    61681 ctttcgcggg aggattattt tgctgctcgg tactggcctg aaagcctttt tgcaacatct
    61741 cataggcgga ttcccggtcc acctcatcct catatttgcc atacaccgga gagtgattaa
    61801 tcaagccatt acgctcatct tccgtcaccg gccccatccg cgaacaaggc gcgatcacca
    61861 tcgcacgctc caccacagaa gggcttcctt tcgcatccag aaaagagatc aacgcctcgc
    61921 cggtgcccag ttcctgaatc gccttttcgg tatcaaacgc cggattggcc cgcatggttt
    61981 gcgccgctgc cttcactgct ttctgatctt ttggcgtaaa agcccgcaaa gcatgttgaa
    62041 cgcgattacc gagctgcccg agcacattat ccggaatatc agacgggttt tgcgaaacga
    62101 accagacgcc cacgcctttt gagcgaataa gccttatcac ctgctcaatc ttatccagca
    62161 gtacctgcgg ggcatcgtta aacagcagat gtgtctcgtc gaagaaaaac accagttttg
    62221 gtttctccag atcgcccgct tccggcaatt gttcatacaa ctctgaaagc atccacagca
    62281 ggctggcggc gtacagtttc ggcatctgat aaagtttctc ggcgctgagg atattgataa
    62341 cgcctttacc gttggcatcg gtgcgcatcc agtctttgat atccagcatc ggctcaccaa
    62401 agaagtgtgc tgcgccttgc tgttccagcg acaacagccc gcgctggatg gcaccaaccg
    62461 atgcgctact gatattaccg tactgattct ggaaggattt ggcgttatcg ccgatgtact
    62521 gggtaattgc ccgcagatct ttaaagtcga gcagtaacag cccctgatcg tcagcaatgc
    62581 ggaagatgat attcagcacg ccagattgca catcgttgag attcaacagt cgtgccagca
    62641 acagcggccc cagatccgaa accgtcgccc gcaccggatg gcctttctcg ccaaagatat
    62701 cccacaccac caccggattg gcatgcggtt gccagtcatt gacgccgata tttttaagcc
    62761 ttgcgagcag tttttccgac accgttcctg cctgcgcgac gccggtcaga tcgcctttca
    62821 catcagccat aaacaccggc acgccgattt ccgacaatga ctccgccagt ttttgcagcg
    62881 taacggtttt acccgtcccc gttgcgccgg taatcagccc atgacggtta gccattcccg
    62941 gcagtaaaaa cagttctgtg tccggcgtgc gggcaattaa caggggttca ctcatgagat
    63001 atcctccagt ttcctgcctg gagtataggc aacgtccggc aaagagaata cgtgtaatga
    63061 agagaaaata acctaaagcg tgttgccgtg ataaatctga tacccgaggt caacagtgtt
    63121 gtggttgtga tcgttatttt taattttgct cagcaacatt tgggcggcca tccgaccaat
    63181 atcaaaacgg ggagtaatta cgctggcgag gcttgggatc atttgcctgc caatctccag
    63241 cccatgaaaa cccgcaatgg aaatttgttc tggcacggcg aggtttcgct cgcggcacag
    63301 cagaagcgca cccatcgcaa tatcgtcatt ggtgcaaaat acaccgtcta aatccgggtt
    63361 tgcgctaagg gcatcacgca ttaactgcat tcctaaatga atggatgaga tggcgcgtgg
    63421 attcatgcgt aatggggaaa gattatgcag catcatcgca tcgcaatacc cctgataacg
    63481 ctgttcatcg cgggtgtcat ctttggaacc gagatacaga attttgtgcc tgactcgctt
    63541 ctccagcatg gtacacacca tgtcaaaagc ggcctgccga ttatcaaaac cgacctccat
    63601 atccagccgt tctccctgta catccatcaa ttccacgacc ggaatggtgg ctgaacgcag
    63661 aaatttcacc gtcctgatgg tgtgatattt ttccgaaagt ataatcccgt caatgttata
    63721 agagagcagg ttaatcaccg actcttcttc ggaatcgcga tcgtaattgt aattagcaat
    63781 aagggtctga taattatgct cagaggtgac ggattctatt cccgcgagaa tgtcagcgaa
    63841 gagttgattt tgaaatgacg gaatcaagat accgagggta taactttgtg cattcaacaa
    63901 catgccggga gcgcgattag ggatataatt gatctcttcc ataattttgg cgatgcgctc
    63961 gcctgtttcc tttgccacct ttttcggcga gcggatataa cgactcacgg tcatttttgt
    64021 tacgccagcc agcgtagcga tatcctgtaa agaaattctg tgattgcgca taacttctcc
    64081 caacgtcggg ggaatcttca atcttccccc gaaactacat cacaatattt tattctttca
    64141 gtgcaatacg gcgttaatgg ctaacacgcc cagcaaaccc ataatagaaa taagggtttc
    64201 catcaccgtc caggtacgca acgtttcacc aaccgtcaga ttaaaatacc ctttaaatag
    64261 ccagaagcca gggtcgttta cgtgtgacgc gatcacgctg cccgcaccgg ttgccagtac
    64321 cattaaagcg ggatcggcat gggtaacgtt gataatcggc aacaccacac ccgcggtggt
    64381 aatggccgcg acggtggcag agcccagagc gatacgcaac agcgccgcaa cagtccagca
    64441 catcaataac ggcgaaagtg tagttccggt cattaagtgt gaaatatagt gcccgacacc
    64501 gctatctact aatacctgct taaacgcgcc gccgccagcg ataataaaca caatcatcgc
    64561 gatagcgcct atagaatccc caatgatatc catgatttgc tcgatggtgc gtccattacg
    64621 tcggcccagt gtgaaaatcg caataacaat ggcaataaac agcgcaacgg cagggttacc
    64681 gacaaattca aagaagaggc gcacggtgtt agttttcggt aacgtaattt cacaaacggc
    64741 ggcgatagcc atcaggatga ccgggatcac ggcagcgaaa atactgttcc agaaggaggg
    64801 catctcctct tcgctaaaca gatgaggatt aaataagcct tccggtggtg ctttctcaaa
    64861 gcgagttagc agtttagaaa acagcggtcc tgcgacaata actgtcggaa tggtaatgat
    64921 aaatccatac agtaaagtcg ttccgagatt agcctcaaag atagtcgcga tggcagtagg
    64981 ccctggatgt ggcggcagaa aacagtgggt tacagagagc gctgctacca ttggtacgcc
    65041 aacatacaac aggggtaatc ctgatgatgc tacgatggta aataccaacg gcaacagcag
    65101 gacaaaaccc acttcaaaaa acatggcgag gcccacaacc agaccggtga tcactagcgc
    65161 ccattgcacg cgttttttac caaaagtagc aatcagcgta gtggcgatac gttgtgccgc
    65221 acccgtatca gaaatcagct tgcctaacat ggccccgaaa ccgaggatca ttgccagccc
    65281 gccgagcgtg ctgccgatac cattttgtat agagtgcagg acggcctgtg catccatccc
    65341 ttcggcaaat ccgacgacgg cagctaccag aacgagggca ataaagccgt taactttaaa
    65401 gacgatcatc aggataagaa gcagcgcgac gcctgccgca ataatgatta atggcatagt
    65461 ctttatctct tattagtgcc agaaaagcag cgctttcgcg ctgcacgtag ggggggctgt
    65521 taaacagcca ctaacatgcc gccatcaaca aacaacaggt ggccgtttac gaaatcagag
    65581 gcttttgaag aaaggaacac cgcagcacca atcagttcct gcggatctcc ccagcgtgcg
    65641 gcgggggtcc gtttgcacaa ccaggcggtg aaggcttcgt cctcaaccag tgctttagtc
    65701 atttctgttt tgaaatagcc cggcgcaata ccgttgacct gaatattgtg gcgcgccagc
    65761 tcgacgcaca tgccgcgggt gagcattttt accgcccctt tcgatgcggc ataaggggtg
    65821 atggtgtcac gtcccagttc gctttgcatc gagcaaatat taataacttt acctgccttg
    65881 cgttcaacca tgtgacgagt taccgcttgc gataccagga acacggcggt ctggtttact
    65941 gcgatcacat cattccactc ttgttcaggg aactcagtaa aaggatgacg gcgctggata
    66001 ccggcgttat tcaccagcac atcaatgggg ccgatgtcct tttcgatatg ttcaacggcg
    66061 gcatcaattt catgtttatg agtaacatta aaaggtgcgg caacggcctg aataccctcc
    66121 tggtggagtt tttctacagc aagttctgcg cgttcggcag taatatcatt aataattatt
    66181 tgtgcgccat atttacccag gccggttgcc agtaaaaagc caatgccctg tgctgaaccg
    66241 gtaatcaaga tattttttcc tgccagtgaa aatagatcgt tcattgtatt attcctttat
    66301 ttatctctta gaaaacaagc tggacttttg ctgcctgggt tttatcaccg gcgaaacgta
    66361 gcgcctcttc caggtcagtg aagggatatt cagcactcag taaaggcagt ggattgataa
    66421 cgccattcgc cagccatgac actgcggtat taaattcgct ggtaaaacgg aaagagcctc
    66481 tgagtgaaat ctccttacca atcaacgtca tcattgggaa ttctgccatc gcgcctccca
    66541 tacctacctg caccattacg ccgcgtgcac gagtgacctc cagacaggta ttcactgatg
    66601 aaggatgacc ggacacttca aagctgacat cgaaatagcc tttttccgct ttccagtgat
    66661 ccatgtcgtc gttttgtggg tttacgagca catccgcccc catctctttg cccagcgaaa
    66721 gggaacgggg actcacatca gcacagacaa tttccgcggc ccccagtgtt ttcactgcac
    66781 tgacaatcag gcagccaatg ggtccaacac cggaaataaa tactcgcttg ccctgtaact
    66841 cgccggcctg atgtgcggca tgaatcgcga cggctaaagg ttcggcaaaa gccataacct
    66901 tttcgtcagc tttggccgga taagggacac attgcgacgt ttcgaccatt ttataacggg
    66961 taaaaccacc atcaacatga gggaaataca tggcactgcc aaaaaaacgc atatctgtac
    67021 actgattctc gttatgttca atgcagtatt tgcagtgacc gcacggttta gacggattaa
    67081 tggctaccgt ttgcccttca tgtaattctg atgagtcgct atgaataact ttaccgataa
    67141 cttcatgacc taacaccatc ggtgccttta tcatgaaatt acctactttt ccttcctgat
    67201 aataatgtaa atcggaaccg caaattccac ctcgggttat ttgtactaat gttccattat
    67261 tattccaatc tatcgtctgc tcggtaacgg caacagtttt cttgcccgca acaacgcagg
    67321 actgtgtttt cacttgcata aattcctcac tggtcaggta gacacctcgg aagcatttaa
    67381 gcggttttaa ctgtcattat ttgtgatgaa gatcacgtca gaaaattgtt acattactat
    67441 gttacgcata acgtgatgtg ccttgtaatt cttatcagta gaaataaaaa aacgtgaaat
    67501 tattatgccg ccaggcgtag tatcgcagca ggtaagatga ttcaggagat tttaaatggc
    67561 gggtgaaagc tttattttga tgggcgtttc agggagtggt aaaacattaa ttggtagcaa
    67621 ggttgccgcg ttattatctg ctaaatttat tgatggtgac gatcttcatc cagccaaaaa
    67681 tatagataaa atgtcgcagg gtattccatt atctgatgaa gatcgacttc cctggctgga
    67741 acgcttaaat gatgcttcat acagtcttta taaaaagaat gaaacaggat ttattgtctg
    67801 ttcatcatta aaaaaacagt atcgtgatat tttacgcaag ggtagccccc atgttcattt
    67861 cctctggtta gatggcgact atgaaactat tctcgcgcga atgcagcgtc gggctgggca
    67921 ttttatgccg gtagcgttac taaaaagtca gtttgaggca ctggagcgtc cacaagcaga
    67981 tgaacaggat attgtgcgca ttgatatcaa ccatgatatt gccaatgtca ccgaacaatg
    68041 tcggcaggct gtgctggcga tacgacaaaa cagaatatgt gcgaaagagg gcagcgcctc
    68101 agatcagcgc tgcgaatgat tttcaataat cggctttcaa caccacgcgg taacgcgcct
    68161 taccgtcgcg cacatgctgg atggcgtcgt taattttcga catcgggaac agttcggtgg
    68221 tcggcgcaac cttgctgcgg gcggcaaaac gcatcagctt acgcagctca taaggcgtgc
    68281 cggtagcaga accagagacg ctgcgatcgc ccgcaattaa cgtaaaggcc ggaacagaca
    68341 gcggcgtgag aaccgcaccg accgtatgga aattaccgcc ataggtcagc gcctcaaaat
    68401 agggctgcca gtcgaggctg acgttgacgg tgttgataat gagatcaaac tgccccgcca
    68461 gtgctttcag tgcctgcgga tcgcggctat tcaccacttt atcggcaccc atcgccagca
    68521 cttcctgctc tttcgccgga ttagaactaa aggctgtcac ctcgcatccc attgcgtgca
    68581 gaagttttat agcgatatgc cccagcccgc caataccaat taccccaacg cggctggtag
    68641 cagtgatatg gtgcatcaac agtggtttaa agaccgtgat accgccgcac aacagcggcc
    68701 cggcggactc gatatcaata ttttctggca gtggaatcac ccattgccag tccgcacgca
    68761 acttctcggc aaagccaccg cgattcataa tcgtcggcac cgcaccttgc tcgcagttga
    68821 tctgattacc gctaatacag gcgtcgcagt gaccacagct acgcgccgtc cagccaatcc
    68881 cgacacgctg accgacctgc aaacctttat cctgcgcggc gctcccgagt gccaccacgc
    68941 gcccaatcac ctcatgcccg gcaaccagcg gatattgtga aaatccccat tcgttatcga
    69001 tcatcgacag atcggaatgg cagatcccgc agtaatccac ctgcacttca acatcttgtg
    69061 gcctcagctc accgggatcg tactcataaa cttccagttc gccgcccgct tcttttgcgg
    69121 catagctttt tatcatcgac attttttggt ccttctctgg tgttgtttgg gaagtgtaga
    69181 gcatggcagg gcggggtgcc tggagtgtga caaaggttac acatcgctgt atgcaatgct
    69241 gaaaatttca gcacttagcg aggtgcgagc aagctggcgc ttgcatggtg gcgtgcgaca
    69301 ggtataatcc acaacgtttt ccgcatacct cttcagtgcc gaagtggcga aatcggtaga
    69361 cgcagttgat tcaaaatcaa ccgtagaaat acgtgccggt tcgagtccgg ccttcggcac
    69421 caaaagtatg taaatagacc tcaactgagg tcttttttta tgcctgaaat ccagtgttta
    69481 tctatctttc ccgctatatt aactctctca aggtcaaccg atatcaacgt acatctacca
    69541 acatatgttg gtacagatga tggtatttcc ggttcgataa tgcttgtacc aacagggagg
    69601 gaatacgatg gcattaacag atatcaaagt cagagcagcc aagccaacgg ataagcaata
    69661 ttagctgact gatggtggcg gtatgcatct gcttgtccat ccaaatggtt ctaagtactg
    69721 gcgtttgcag taccgttatg agggaaagca aaaaatgctg gcacttgggg tttatcctga
    69781 aatcacacta gcggatgcca gagtacgtcg tgacgagacg cgtaagctgc ttgcgaatgg
    69841 cgtcgatccg ggagacaaaa agaaaaatga taaggttgaa cagagtaaag cacgaacctt
    69901 taaagaagtc gcgattgagt ggcatggcac caataaaaag tggtctgaag atcacgccca
    69961 tcgtgtgcta aaaagtcttg aagataatct ttttgcagcg cttggtgaac gtaatatcgc
    70021 tgagttaaaa actcgagatt tattagcacc tattaaggcc gtagaaatgt ctggacgtct
    70081 tgaagtggcc gctcgtcttc agcagcgcac tacagccatc atgcgctatg cagtgcaaag
    70141 tgggttaatt gattataacc cggcacaaga gatggctggg gcggttgctt cctgtaatcg
    70201 acaacatcgt cccgcgcttg aattaaagcg catccctgag ttgcttacaa aaatagatag
    70261 ctatactggt aggccgctaa cccgatgggc gatagaactc actttgctga tctttattcg
    70321 gtccagtgag ctgcgttttg ctcgttggtc agagatcgat ttcgaagcgt ctatatggac
    70381 tatcccaccg gagcgggagc ctattcctgg agtgaaacat tcccatagag gctcaaaaat
    70441 gcgtacaacg catctagtgc ctctttcaac gcaagctctt gcaattttaa agcagataaa
    70501 acagttttat ggggcccatg acttgatatt tattggtgat cacgattcgc acaaacccat
    70561 gagtgagaat acggtaaata gtgcgttacg ggtcatgggg tatgatacaa aagtagaggt
    70621 ttgtggtcat ggctttcgaa caatggcctg tagttcattg gtcgaatcag gtctgtggtc
    70681 tcgtgatgct gttgaacgtc agatgagcca catggcgcga aattcagtga gggccgcgta
    70741 tatccataaa gcagagcatc tggaagaacg gcgattgatg ctacagtggt gggccgattt
    70801 tctggatgta aacagagaaa ggtttatcag tccatttgaa tatgcaaaga ttaataatcc
    70861 attaaaacag taatcatccc gggcaaatgc ccgggaatta ttctaggatt attttctttg
    70921 ttaaaaaaga caaacggtat taactgatgt atttactatt taccgctccc tgcagggggg
    70981 attatcagaa tccgcggtta ttcattattt atatacactc aataaaaaag taacctcata
    71041 aaaaatcctg tactcacagg gtgatgcggg ataactgacg taaggagtgc tgcagggatt
    71101 tgatggtccc ttgtgctgat atgaatacgg atattttata cagaatactt ttcagtacaa
    71161 gcgactgtat tacagtctgc catatatgat tacctgtgat caggaagaat aagtggctga
    71221 ggtttcaaac tgtctgccgg tatattcctc tctcccttaa aaaccataac aggttagtta
    71281 tcttcgtctg tcagcggatt gaatggcggt atattttcag tacaaatacc ggtcatccac
    71341 tgaaaaatac ctgcgaaatg acgggcactg atttttctac tgacgggctg atgagacgtt
    71401 atgtcactgg cggtaataat cagtggcaca ctgtagcctc cctgcacatg accataatga
    71461 tgaacaggat tggcactgtc gctgaccgac agcccaaggc cagaaaagta aagcatggcg
    71521 aaatgacgtg agtaccggcg aaggatacca tcaagctggc caagaaagtt atccagttta
    71581 ctgatgctgg cgaggtaaca ggcaattttt cggggatact gctccaggta attattcggc
    71641 taggagttaa ggctgtcaca cggatttgga tgagaaccca tcatgtgcag gaaaattatc
    71701 ttcggagagg atgtatccgc cagcgcacgt tctgtttcct gtaacaacag tttgtcatct
    71761 gttttacggg aagcgaagct gcctttcttg aggaaagtgg catgctccgc atcagaagca
    71821 ataacagaga tgcgtgtgtc atgctccccc agttttcctt gattggatat ccaccatgtg
    71881 ctgtatcctg cttttgctgc cagcgccacc acattgttgc cggaatcagg gttctgctca
    71941 tagtcataaa tcagtgtccg gctcagggaa gacactgtac tggaggctgt cgaggtataa
    72001 tcgtcaataa ataaaccggg tgccgtattc agccacggtg tggttggtac gggatagtca
    72061 tacactgaca tataatccct gcgcacactc tcaccagtga caataacaat cgtatcatat
    72121 aacggtgctc ccggctggat tttccagttg tcagccccgt gctactcagt tgttcataac
    72181 gctgcatttc acgtaatgtg tcagttgtcc ccacaactgt tcctttaacc atccgcaatg
    72241 gccaacggtt tacagagcat aatacgaata gccgtagagc cagccagtta cggtggtcgc
    72301 gatggtgtgt ccgccagaaa atcaccacga ataccagaat cgcggcactg accagaaaat
    72361 gataaacagg aaacacgggg cggttcactt tgctttctcc tgtagtgttt tctgtaggtt
    72421 ctgaaccggt tctagcgggg ttgtgaggct gatggcaatc gccgggctct tcctgcatgt
    72481 tacactggta ctgcaagagg gggccttcgc tcctctgaca ttactcagta ttttcactac
    72541 tacaccccat ctgccatcca taacaaccga ttcaccccct cgcgaggcgt tgttgcccgt
    72601 cacccggaca gacaccgtta tggacttctc ttgttgcacg atcgatcaat tgttatgcat
    72661 gctgacacca caaccccggt gggtatgacc gatgcctttc tcactctctt cccggtcatc
    72721 gtcggccttc cccgttattg tggcgtgtca gcaccctcga cggcatttcg atggctgctc
    72781 agtgttcact cacgttacgg cccgcataac ccgttgactt cctgaagaag tcgtttcagg
    72841 gagagattca gcccttttgt tacctcctgg accgcttctt gtccttccag tcggagcgag
    72901 agttagccgg ggcggacttt caccgacgga aagatcgtgc atttcaaggc acacacaaca
    72961 atatctgcga gcgtgctatc cgtccggtcg ttatgggacg aaaggcctgg ttgttcgctg
    73021 gttcactggt ggccgggaac cgcgcggcac agataatgag ccttctggga accgcagtct
    73081 ggagccacat gcttggctga cggacgtcct gacgcgtctg ccggagtggc cggaggagag
    73141 gttggctgag ttactgcctc ttgagggctt taccttcttc gggtaagtga tacctgccgt
    73201 caggtgtttg tgcaccgggc cataacctgc agttgggaat tgaacgcctg acaacaggaa
    73261 atgagctaga gcagtaaaca cgcgggctga caatgggtca gaatatgagt tagcccggcg
    73321 ttattcctgg ggtcagtact tcagatctag cctccggtac cagactagct agcccctacg
    73381 caaccaaata aatcaaccat cgtcagagtg caatcgttca tatacactgt cggtgatgcg
    73441 ctaaaaaatg cgttaatgtt gagttcctcg aacccttacc aattaaggga catagccaga
    73501 tacgcaactg gctgatgcaa gcagtctaat gtctttagat ccgctggcct gcgggcatga
    73561 caatgtcaaa cagtcaaaga ttgtggcgtt gacggtcagt cttggggaca aaaaagctca
    73621 gtattctcgt tggcaaccag ataatttaac agttgatgct gaaacgaaat aggcatcttg
    73681 gttctgtaat gccagtcagt taagcaactg actggctctt tttcggggct gtggggtatt
    73741 tccagggcct ctcctttacc actctcggga aggccctttc ccttcttgtc ggtaatttca
    73801 caagttgtcc catacttgca agatcgcgca tcagctccgg tatacgtccc ggtgaagcgc
    73861 cctgcaatgt catcagcatt ctcatcacca ttccgcatga ttctgagaaa ctcagttgat
    73921 tcggccagta acctttcaga tgttccgcca ttttaatcat ctgatatctc accagattat
    73981 aagccagtaa gacaccccac ag
//
LOCUS       DAPOZO010000007        72891 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPOZO010000007 DAPOZO010000000
VERSION     DAPOZO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.007"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            302..1687
                     /gene="dcuC"
                     /locus_tag="ROI21_000511"
     CDS             302..1687
                     /gene="dcuC"
                     /locus_tag="ROI21_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDX5109480.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1728..2408)
                     /gene="dpiA"
                     /locus_tag="ROI21_000512"
                     /gene_synonym="citB"
     CDS             complement(1728..2408)
                     /gene="dpiA"
                     /locus_tag="ROI21_000512"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDX5109481.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2377..4035)
                     /gene="dpiB"
                     /locus_tag="ROI21_000513"
     CDS             complement(2377..4035)
                     /gene="dpiB"
                     /locus_tag="ROI21_000513"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDX5109482.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4415..5473
                     /gene="citC"
                     /locus_tag="ROI21_000514"
     CDS             4415..5473
                     /gene="citC"
                     /locus_tag="ROI21_000514"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDX5109483.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5488..5784
                     /gene="citD"
                     /locus_tag="ROI21_000515"
     CDS             5488..5784
                     /gene="citD"
                     /locus_tag="ROI21_000515"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDX5109484.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5781..6689
                     /gene="citE"
                     /locus_tag="ROI21_000516"
     CDS             5781..6689
                     /gene="citE"
                     /locus_tag="ROI21_000516"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDX5109485.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGIELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6700..8232
                     /gene="citF"
                     /locus_tag="ROI21_000517"
     CDS             6700..8232
                     /gene="citF"
                     /locus_tag="ROI21_000517"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDX5109486.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8236..8787
                     /gene="citX"
                     /locus_tag="ROI21_000518"
     CDS             8236..8787
                     /gene="citX"
                     /locus_tag="ROI21_000518"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDX5109487.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQVALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8762..9640
                     /gene="citG"
                     /locus_tag="ROI21_000519"
     CDS             8762..9640
                     /gene="citG"
                     /locus_tag="ROI21_000519"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDX5109488.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEEVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQSITPITICATAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9690..11153
                     /gene="citT"
                     /locus_tag="ROI21_000520"
     CDS             9690..11153
                     /gene="citT"
                     /locus_tag="ROI21_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDX5109489.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11267..12073
                     /gene="rna"
                     /locus_tag="ROI21_000521"
     CDS             11267..12073
                     /gene="rna"
                     /locus_tag="ROI21_000521"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDX5109490.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGNTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12303..12713
                     /gene="rnk"
                     /locus_tag="ROI21_000522"
     CDS             12303..12713
                     /gene="rnk"
                     /locus_tag="ROI21_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDX5109491.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            13069..13497
                     /gene="uspG"
                     /locus_tag="ROI21_000523"
     CDS             13069..13497
                     /gene="uspG"
                     /locus_tag="ROI21_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDX5109492.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(13618..15183)
                     /gene="ahpF"
                     /locus_tag="ROI21_000524"
     CDS             complement(13618..15183)
                     /gene="ahpF"
                     /locus_tag="ROI21_000524"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDX5109493.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVEIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVGRNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(15354..15917)
                     /gene="ahpC"
                     /locus_tag="ROI21_000525"
     CDS             complement(15354..15917)
                     /gene="ahpC"
                     /locus_tag="ROI21_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDX5109494.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            16289..17035
                     /gene="dsbG"
                     /locus_tag="ROI21_000526"
     CDS             16289..17035
                     /gene="dsbG"
                     /locus_tag="ROI21_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDX5109495.1"
                     /translation="MLKKILLLALLPAIAFAEELPDPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATTAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            17243..18146
                     /locus_tag="ROI21_000527"
                     /pseudo
     CDS             17243..18146
                     /locus_tag="ROI21_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            18293..19513
                     /locus_tag="ROI21_000528"
     CDS             18293..19513
                     /locus_tag="ROI21_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDX5109496.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWIALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YASHQRLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            19486..20115
                     /locus_tag="ROI21_000529"
     CDS             19486..20115
                     /locus_tag="ROI21_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDX5109497.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(20116..21276)
                     /gene="ybdL"
                     /locus_tag="ROI21_000530"
     CDS             complement(20116..21276)
                     /gene="ybdL"
                     /locus_tag="ROI21_000530"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDX5109498.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVDQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     QAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            21384..22472
                     /gene="hcxA"
                     /locus_tag="ROI21_000531"
     CDS             21384..22472
                     /gene="hcxA"
                     /locus_tag="ROI21_000531"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDX5109499.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSLNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNHQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTEAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(22482..22679)
                     /locus_tag="ROI21_000532"
     CDS             complement(22482..22679)
                     /locus_tag="ROI21_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDX5109500.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(22792..24897)
                     /gene="cstA"
                     /locus_tag="ROI21_000533"
     CDS             complement(22792..24897)
                     /gene="cstA"
                     /locus_tag="ROI21_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDX5109501.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(25078..25491)
                     /gene="entH"
                     /locus_tag="ROI21_000534"
     CDS             complement(25078..25491)
                     /gene="entH"
                     /locus_tag="ROI21_000534"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDX5109502.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLDDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(25494..26240)
                     /gene="entA"
                     /locus_tag="ROI21_000535"
     CDS             complement(25494..26240)
                     /gene="entA"
                     /locus_tag="ROI21_000535"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDX5109503.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(26240..27097)
                     /gene="entB"
                     /locus_tag="ROI21_000536"
     CDS             complement(26240..27097)
                     /gene="entB"
                     /locus_tag="ROI21_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDX5109504.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(27111..28721)
                     /gene="entE"
                     /locus_tag="ROI21_000537"
     CDS             complement(27111..28721)
                     /gene="entE"
                     /locus_tag="ROI21_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDX5109505.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESQAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(28731..29906)
                     /gene="entC"
                     /locus_tag="ROI21_000538"
     CDS             complement(28731..29906)
                     /gene="entC"
                     /locus_tag="ROI21_000538"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDX5109506.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPDQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            30095..31051
                     /gene="fepB"
                     /locus_tag="ROI21_000539"
     CDS             30095..31051
                     /gene="fepB"
                     /locus_tag="ROI21_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDX5109507.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIVAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAINAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIVQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(31055..32305)
                     /gene="entS"
                     /locus_tag="ROI21_000540"
     CDS             complement(31055..32305)
                     /gene="entS"
                     /locus_tag="ROI21_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDX5109508.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            32416..33420
                     /gene="fepD"
                     /locus_tag="ROI21_000541"
     CDS             32416..33420
                     /gene="fepD"
                     /locus_tag="ROI21_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDX5109509.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGILAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGVTALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            33417..34409
                     /gene="fepG"
                     /locus_tag="ROI21_000542"
     CDS             33417..34409
                     /gene="fepG"
                     /locus_tag="ROI21_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDX5109510.1"
                     /translation="MIYVSRRLIITCLLLITACVVAGIWGLRSGTVTLETSQIFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            34406..35221
                     /gene="fepC"
                     /locus_tag="ROI21_000543"
     CDS             34406..35221
                     /gene="fepC"
                     /locus_tag="ROI21_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDX5109511.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(35218..36351)
                     /gene="fepE"
                     /locus_tag="ROI21_000544"
     CDS             complement(35218..36351)
                     /gene="fepE"
                     /locus_tag="ROI21_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDX5109512.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQALEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYNSWTLSFTAPTSEEAQTVLRGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDLDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSAMIGGMVACGGVLLRHAMASRKQDAVMEEHLV"
     gene            complement(36567..40448)
                     /gene="entF"
                     /locus_tag="ROI21_000545"
     CDS             complement(36567..40448)
                     /gene="entF"
                     /locus_tag="ROI21_000545"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDX5109513.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALVALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIEAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYVQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYLEMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVETGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSA
                     ALHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAIMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTTALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFTRQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTDLFTTIEGNYADAVRLLTTAHSVPFEGKATLFVAERTLQEGMNPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(40445..40663)
                     /gene="ybdZ"
                     /locus_tag="ROI21_000546"
     CDS             complement(40445..40663)
                     /gene="ybdZ"
                     /locus_tag="ROI21_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDX5109514.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            complement(40666..41868)
                     /gene="fes"
                     /locus_tag="ROI21_000547"
     CDS             complement(40666..41868)
                     /gene="fes"
                     /locus_tag="ROI21_000547"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDX5109515.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEITFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            42111..44351
                     /gene="fepA"
                     /locus_tag="ROI21_000548"
     CDS             42111..44351
                     /gene="fepA"
                     /locus_tag="ROI21_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDX5109516.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            44526..45146
                     /gene="entD"
                     /locus_tag="ROI21_000549"
     CDS             44526..45146
                     /gene="entD"
                     /locus_tag="ROI21_000549"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDX5109517.1"
                     /translation="MKTTHTALPFAGNTLHFVKFDPASFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAGVYGSISHCGATALAVVSRQ
                     PIGIDIEEIFSAQTAAELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKASKI
                     QAAQGFLDYQIISWNKQQIIIRLEDEQFAVHWQIKEKIVITLCQHD"
     gene            complement(45267..45419)
                     /gene="hokE"
                     /locus_tag="ROI21_000550"
     CDS             complement(45267..45419)
                     /gene="hokE"
                     /locus_tag="ROI21_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDX5109518.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVRDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(45771..45923)
                     /gene="hokE"
                     /locus_tag="ROI21_000551"
     CDS             complement(45771..45923)
                     /gene="hokE"
                     /locus_tag="ROI21_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDX5109519.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            46364..47482
                     /locus_tag="ROI21_000552"
     CDS             46364..47482
                     /locus_tag="ROI21_000552"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDX5109520.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLMEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADSGSLIGLVKKHCEIWAGD"
     gene            47548..47796
                     /locus_tag="ROI21_000553"
     CDS             47548..47796
                     /locus_tag="ROI21_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDX5109521.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            47861..48229
                     /locus_tag="ROI21_000554"
     CDS             47861..48229
                     /locus_tag="ROI21_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDX5109522.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            48323..48976
                     /gene="nfsB"
                     /locus_tag="ROI21_000555"
     CDS             48323..48976
                     /gene="nfsB"
                     /locus_tag="ROI21_000555"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDX5109523.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAQWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            49084..50331
                     /gene="ybdG"
                     /locus_tag="ROI21_000556"
     CDS             49084..50331
                     /gene="ybdG"
                     /locus_tag="ROI21_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDX5109524.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(50399..51775)
                     /gene="pheP"
                     /locus_tag="ROI21_000557"
     CDS             complement(50399..51775)
                     /gene="pheP"
                     /locus_tag="ROI21_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDX5109525.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(51877..55020)
                     /gene="cusA"
                     /locus_tag="ROI21_000558"
     CDS             complement(51877..55020)
                     /gene="cusA"
                     /locus_tag="ROI21_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDX5109526.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRAYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     TFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEVEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(55032..56255)
                     /gene="cusB"
                     /locus_tag="ROI21_000559"
     CDS             complement(55032..56255)
                     /gene="cusB"
                     /locus_tag="ROI21_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDX5109527.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWITAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNTDEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(56271..56603)
                     /gene="cusF"
                     /locus_tag="ROI21_000560"
     CDS             complement(56271..56603)
                     /gene="cusF"
                     /locus_tag="ROI21_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDX5109528.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETTSEAKPQVISATGVV
                     KGVDLESKKITIHHDPIVAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(56627..58009)
                     /gene="cusC"
                     /locus_tag="ROI21_000561"
     CDS             complement(56627..58009)
                     /gene="cusC"
                     /locus_tag="ROI21_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDX5109529.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPKQFSLSQNGLVN
                     AADNYQNAGWRTFFIDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            58166..58849
                     /gene="cusR"
                     /locus_tag="ROI21_000562"
     CDS             58166..58849
                     /gene="cusR"
                     /locus_tag="ROI21_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDX5109530.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            58839..60287
                     /gene="cusS"
                     /locus_tag="ROI21_000563"
     CDS             58839..60287
                     /gene="cusS"
                     /locus_tag="ROI21_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDX5109531.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLKRVLNHPEETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     GAPDIREFTRDAIPDKDTRGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            61024..62925
                     /locus_tag="ROI21_000564"
     CDS             61024..62925
                     /locus_tag="ROI21_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDX5109532.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPRR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            62953..63414
                     /locus_tag="ROI21_000565"
     CDS             62953..63414
                     /locus_tag="ROI21_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HDX5109533.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            63434..68262
                     /locus_tag="ROI21_000566"
                     /pseudo
     CDS             63434..68262
                     /locus_tag="ROI21_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522504.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
     gene            68259..68792
                     /locus_tag="ROI21_000567"
     CDS             68259..68792
                     /locus_tag="ROI21_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109534.1"
                     /translation="MTIMLTLENETNYKDVVVNIDEFVPFDITFGKGSEQCLYWRGGN
                     GKSSLVEVGLNMYSGEVSNITLANIDSGEINITDEMLANNLPEIQGSVIFQTSEWLNK
                     DISEYSNRFIDDFDCNIKLIIGSNYIKLLFKKATNPICYIINRNIRFGFSLSRELSTL
                     EILDLNQEKLDILKLFK"
     gene            69284..69715
                     /locus_tag="ROI21_000568"
     CDS             69284..69715
                     /locus_tag="ROI21_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109535.1"
                     /translation="MKIEANSLTHPYDYNASLNNYLHYFENFSRNDWNKDSCGGYFTF
                     RDDDRECVLFFVNYGDNFSLRYDCTQSSDGIVSCWYSNHEDDKMNIIVDADTEQFIPL
                     GSCLPPDITLSVIKDFFKNPLEKSKKVKWMNADDIDWSSVY"
     gene            70327..70542
                     /locus_tag="ROI21_000569"
     CDS             70327..70542
                     /locus_tag="ROI21_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109536.1"
                     /translation="MVVIEDNDLYDPIKAEGVDGWMYYKFILSIFPLKGVDTTLEYQR
                     ELSFLFLKKLKDAGLLGELICEDDFHD"
     gene            70608..70901
                     /locus_tag="ROI21_000570"
                     /pseudo
     CDS             70608..70901
                     /locus_tag="ROI21_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            71039..71401
                     /locus_tag="ROI21_000571"
     CDS             71039..71401
                     /locus_tag="ROI21_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109537.1"
                     /translation="MNKNEIHFPIGYGWLNEKKIIGTSEFSQLEPWFYLDENRCFFAN
                     KKWPNVIGEQLFAFAKRQDNDELACFKINESGVCEGVYLINGWTPNGFDIIQFYPDFW
                     EWVHLVIQDVSEWIDNKG"
     gene            71431..72129
                     /locus_tag="ROI21_000572"
     CDS             71431..72129
                     /locus_tag="ROI21_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HDX5109538.1"
                     /translation="MKVTWSGGRSSLPADTDYPDPRGERDAEADPGLLYAGQWQDAES
                     GLCYNRFRYYEPETGMYLVSDPLGLLGGEQTYRYVPNPSGYVDPLGLATCPILRQWAL
                     TNLEASRAARAASNFGKPLVQRNKTIGDKVRDMIAKERGTTLIEQNYRVTGGLKRIDV
                     VDGVTGIESKVGRTGLTTRVRQEVARDIKILRSEQLDQIEWVFTRSSTTGKIGPTKPL
                     EDLLNKHGIPIIYR"
     gene            72141..72698
                     /locus_tag="ROI21_000573"
     CDS             72141..72698
                     /locus_tag="ROI21_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4304 domain-containing protein"
                     /protein_id="HDX5109539.1"
                     /translation="MKLKPIGDFLNKNGFIKNGNNDFYKVHDKFVNCINFQKKSTGDI
                     FFINTGVHPVFENFCNEPPKKEIDCYIRNRVCDGLSISLLHSFEGSEFVINEIKNKAF
                     CFFEYFDSLNKIFDAIRVVDIEERNICDELSGVSSVRLALMCMNYQYEKKKFSLVSDF
                     ANYGLAISRGPVPKKAFKDMIKITE"
     gene            72705..>72891
                     /locus_tag="ROI21_000574"
     CDS             72705..>72891
                     /locus_tag="ROI21_000574"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109540.1"
                     /translation="MRCNTCCLCTAKINGREETILYNRDATGRLAEVITPEGKTQYAY
                     DKSGRLTGIFSPDDTSQR"
BASE COUNT        17955 a        19178 c        18528 g        17230 t
ORIGIN      
        1 tatatatcgt caacctatgc tcatgttaga ttattttttg agcgcacagc cgcagctaac
       61 accattgcaa ttaacaaatt tgcatcaatc caccatcaat ttacacacat tattatgtga
      121 taattgccaa ccgctaaata tgcgttttgt tatctatgta taaaaacagc aaattcaatg
      181 tcttaatggc agtttttctt gattttaatc agcattcatc gccaatttat tgggcatatt
      241 ttttccttaa gctttaggaa ttttttatta tttactttgg ggcctggaga caggaaaaat
      301 tatgctgaca ttcattgaac tccttattgg ggttgtggtt attgtgggtg tagctcgcta
      361 catcattaaa gggtattccg ccactggcgt gttatttgtc ggtggcctgt tattgctgat
      421 tatcagtgcc attatggggc acaaagtgtt accgtccagc caggcttcaa caggctacag
      481 cgccacggat atcgttgaat acgttaaaat attgctaatg agccgcggcg gcgacctcgg
      541 catgatgatt atgatgctgt gtggctttgc cgcttacatg acccatatcg gcgcgaatga
      601 tatggtggtc aagctggcgt caaaaccatt gcagtatatt aactcccctt acctgctgat
      661 gattgccgcc tattttgtcg cctgtctgat gtctctggcc gtctcttccg cgaccggtct
      721 gggtgttttg ctgatggcaa ccctgtttcc ggtgatggta aacgttggta tcagccgtgg
      781 tgcagctgct gctatttgtg cctccccggc ggcgattatt ctcgcaccga cttcagggga
      841 tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt gacttcgcct tcaaaacgac
      901 gctgcctatc tcaattgctg caattatcgg catggcgatc gcccacttct tctggcaacg
      961 ttatctggat aaaaaagagc acatctctca tgaaatgtta gatgtcagtg aaatcaccac
     1021 cactgctcct gcgttttatg ccattttgcc gttcacgccg atcatcggag tgctgatttt
     1081 tgacggcaaa tggggtccgc aattacacat catcactatt ctggtgattt gtatgctgat
     1141 tgcctccatt ctggagttca tccgcagctt taatacccag aaagttttct ctggtctgga
     1201 agtggcttat cgcgggatgg ccgatgcgtt tgctaacgtg gtgatgctgc tggttgccgc
     1261 tggggtattc gctcaggggc ttagcaccat cggctttatt caaagtctga tttctatcgc
     1321 cacctcgttt ggttcggcga gtatcatcct gatgctggta ttagtgatcc tgacaatgct
     1381 ggcggcagtc acgaccggtt caggcaatgc gccgttttat gcgtttgttg agatgatccc
     1441 gaaactggcg cactcttccg gcattaaccc ggcgtatttg actatcccaa tgctgcaggc
     1501 gtcaaacctc ggtcgtaccc tgtcacccgt ttctggcgta gtcgttgcgg ttgccgggat
     1561 ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg gtgccggtgc ttgttgggct
     1621 ggtgattgtt atcgttgcta cagagctgat ggtgccagga acggcagccg cggtcacagg
     1681 caagtaaata gtcatgccgg aggcaagttt cctccggctt tatcatgtca cccactgtgg
     1741 tatatgcgtt gcggtctgcc aactttgccg tgaacaattt cagcaataat cagatggcgg
     1801 ctggcgcaat attcaagata acgtctggca gtggtgcggc tgatagtcag tgcctgcgcc
     1861 accgtttctg ccgtatgttg cacaccaggc tctttaaaca gttttcgcac cgcgtttagc
     1921 gtcaaggggt caatgccggt cggcagttcg tctttgggtt caccgcgagc ataggcatta
     1981 aacatctcat caatttgctt ctggctggcg ctatcaatac tttccagcat atgtttacgc
     2041 tggcggaaac gggttagcgt ttgccccagc cgttcatagg caattggctt aatgagataa
     2101 tcaaatacac cacaacgtac ggcttcagac accgtttcca tatcgctggc tgcagtggta
     2161 aacaccacgt cgccgggata atgcgcctgc accagttcat gcagtaaatt aatccctcta
     2221 ccgtcaggaa gatagttatc gagcaagatt agccccggct taaaacgctc gatcatcatt
     2281 cgggcctgcg ccaggtttcc cgccagtaat atctgactga atccgggaat gtgacgaata
     2341 tattccgcat gcatctctgc cagcggcgtt tcgtcctcaa cgatcaatag ggttaatgga
     2401 gctgtcatta ggtttcactt tcggaatata gattgaaaat aaggtaccgc agggatcatt
     2461 atcttcgaga gtgataacac caccgcagcg cgttacgtag ctggcaatca agtacaaccc
     2521 aatgccatgt tcaccgggct cgtcagcacg cgtactgaca ccctgctcaa atattttgtc
     2581 tcgtagagac tctggaacgc cgcagccctg atcggcgact tcaatcacca catcatcgcc
     2641 ttcatcgctg aggaataatt caacgatctt gtttccttca tcgctacgta ggctggcttc
     2701 gaaggcgtta tcaagtaaat tgccaacaat ggctgcaaac tcggtgctgt ccagtcctgg
     2761 cggcagttgc gaaagctgac ttccggggac gatgaccatt tttagcccca gttcgcgggc
     2821 gcgctgcact ttaccaaaaa gcagccccgc cacctggcga tcggcaaacg cctcgcgcag
     2881 gctatcaata agctgttgct gggcctgaga ctccccctgc accatcgcca acacgcgatc
     2941 atactctttc atctgcaaca gaccattgag cgtcgacatc caattgagat gctcgtgtcg
     3001 caatgtgcgc aggctctcga cgtattgttt aatttgcgtc agttgcgcat tgagggtgga
     3061 tatttcgtct ttactacgaa agctgataat ggcccccagc aaatcatcac cagaacgaat
     3121 agcttcccgg ttggcaataa cgctcagacc gttaaagttc gccaccacat cctgacgttt
     3181 ttcatcaatc tgttcggtaa agaaatcggc gggcctgacc acttcagcaa tgggtttacc
     3241 caaccattgc cgtccggggg aactcagccc cagcatcttt cttgcgttac gattgatggc
     3301 ggtaatgtaa ccgtgcggat ccaccgcaat gagcccttca taaaccgaac taaacagagc
     3361 ctcttgctga cggaccacgc gtgcgatttg ctttggctcc atgcccatca tctgccgacg
     3421 gatatgcgcg gccaggaacc acgacagcaa catcagaatc cctaacagca cgacaaacac
     3481 gccagccatc ggtaataaaa actcagcccg ccagctatca attttactca ccaggtagcc
     3541 aatcgacacc acgccgatga cttttccatc gtcatcaaag attggcgttt tggcacgcat
     3601 cgccatgcca attgaccctt tcccggtgat gaagtagctc tcccctttct ccagcgcgcc
     3661 cggtttggtg aactgcatag gataaccaat tttctccgga ttaggatggt aaagacgaat
     3721 cgagtgccga tcgccaatca ccacataatc gaaatcagta tctctttgta atttgtcagc
     3781 gatggtcgcc agcctcttgt agtcacgcgt tttcactgcg ctgatgatac tgtcattaga
     3841 agcaataatt tttgcctgat tcattgccat gtcgcgcaca tgaagcgtta aatagtcctc
     3901 aaaactggcc gtaaaatatt gcgccagggc tgcaatgaca aatattgaga acaccagaat
     3961 cagcaaaaag atacgcagcg gaaatgccag tctttggaaa aatgcaaact gtttattctc
     4021 gttaagctgc aacattgttt tccttgcggg ttgtcccgag cctctttgac ggatgtaatt
     4081 aaaatgttga atcaaaatac atagatatta aatataatcg tggcaaattt tattcattaa
     4141 atcaattaaa tcacttaaac ctgcgggtca tttcgtaatt acattaatta aaaaacttaa
     4201 aaccattaaa taaataaaaa accactaact ctatgtgaaa taaatcaaaa tttcacgctg
     4261 caatactcct taggatgtat agcgaaagga gaaaaagata tacctcgatc gccccccttt
     4321 ctcccaagtg aaaataaaag gttatcagtt tgcaacatcg aacaacattc gttgcaaatc
     4381 gataacaaca tgcaccttca ggatactatt tattatgttc ggcaatgata ttttcacccg
     4441 tgtaaaacgt tcagaaaata aaaaaatggc ggaaatcgcc caattcctgc atgaaaatga
     4501 tttgagcgtt gacaccacag tcgaagtatt tattaccgta acccgcgatg aaaagcttat
     4561 cgcgtgcggt ggaattgccg gaaatattat taaatgcgtt gctatcagtg aatccgttcg
     4621 cggtgaagga ctggcgctga cattagccac tgaattgata aacctcgcct atgagcggca
     4681 cagcacgcat ctgtttattt ataccaaaac cgaatacgag gcgctgttcc gccagtgcgg
     4741 tttttccacg ctgaccagtg tacccggcgt gatggtgctg atggaaaaca gcgccacgcg
     4801 actgaaacgc tatgccgaat cgctgaaaaa atttcgtcat ccagggaaca agattggctg
     4861 cattgtgatg aacgccaatc cctttacgaa tggtcaccgt tatctgattc aacaggctgc
     4921 agcacagtgc gactggttgc atctgttttt agttaaagaa gattcttcac gctttcccta
     4981 tgaagaccgg ctggatctgg tgttaaaagg caccgccgat attccacgcc tgactgtgca
     5041 tcgcggctcc gaatacatca tctcccgcgc tacgttccct tgctacttca ttaaagaaca
     5101 gagcgtcatt aaccattgtt acaccgaaat tgatctgaag attttccgtc agtacctcgc
     5161 tcccgcgctg ggtgtaactc accgctttgt cggtactgaa cccttttgtc gcgttaccgc
     5221 ccagtacaac caggatatgc gctactggct ggaaacgccg actatctccg caccgcccat
     5281 cgaactggtt gaaattgagc ggctgcgtta ccaggagatg ccgatatccg cttcccgggt
     5341 acgtcaactg ctggcgaaaa acgatctcac ggctatcgcg ccgctggtcc ctgcagtcac
     5401 gctgcattat ttgcagaacc tgcttgagca ctcccgccag gacgcggcag ctcgtcaaaa
     5461 gacccccgca tgagaaacag gtgaaaaatg aaaataaacc agcccgccgt tgcaggcacc
     5521 cttgagtctg gggatgtgat gatacgcatc gccccactcg atacgcagga tatcgatctg
     5581 caaatcaaca gcagcgttga gaaacagttt ggcgatgcaa ttcgcaccac cattctggac
     5641 gttctcgccc gctacaacgt gcgcggcgta cagctgaatg tcgatgacaa aggcgcactg
     5701 gactgcattt tacgtgcacg actggaagcc ctgctggcac gcgccagcgg tatcccggct
     5761 ctgccatggg aggattgcca atgatttccg cttcgctgca acaacgtaaa actcgcaccc
     5821 gccgcagcat gttgtttgtg cctggtgcca atgccgcgat ggtcagcaac tccttcatct
     5881 acccggctga tgccctgatg tttgacctcg aagactccgt ggcattacgt gaaaaagaca
     5941 ccgcccgccg catggtttac cacgcgctgc aacatccgct gtatcgcgat attgaaacca
     6001 ttgtgcgtgt aaacgcgctg gattccgaat ggggtgttaa cgacctggaa gccgtcgttc
     6061 gcggtggtgc ggacgttgtg cgtctgccga aaaccgatac cgctcaggat gttctggata
     6121 tcgaaaaaga gatcctgcgt atcgaaaaag cctgtggtcg tgaacccggc agtaccggct
     6181 tgctggcggc gattgaatct ccgctgggca ttacccgcgc agtggaaatc gctcacgctt
     6241 ccgagcgttt gatcggtatc gccctcggtg cagaagacta tgtgcgcaac ctgcgtacag
     6301 aacgctcccc ggaaggaatt gaactgctgt tcgctcgctg ctccattttg caggccgcgc
     6361 gctctgcggg tattcaggcg ttcgataccg tctattccga cgctaacaac gaagccggat
     6421 tcctgcaaga agccgcccac atcaaacagc tgggctttga cggcaaatcg ctgatcaacc
     6481 cgcgtcagat tgatctgctg cataacctct acgcaccgac ccagaaagaa gtggatcacg
     6541 cccgccgcgt cgtagaagcc gctgaagccg ccgctcgcga aggcctcggc gtggtttccc
     6601 tgaatggcaa gatggtggac ggtccggtta tcgatcgcgc ccgtctggtg ctctcccgcg
     6661 cagaactttc cggcatccgc gaagaataag gcaatcaaaa tgacgcagaa aattgaacaa
     6721 tctcaacgac aagaacgggt agcggcctgg aatcgtcgcg ctgaatgcga tcttgccgct
     6781 ttccagaact cgccaaagca aacctaccag gctgaaaaag cgcgcgatcg caaactgtgc
     6841 gccaacctgg aagaagcgat tcgtcgctcg ggtttacagg acggtatgac ggtttccttc
     6901 catcacgctt tccgtggcgg tgacctgacc gtcaatatgg tgatggacgt catcgcgaag
     6961 atgggcttta aaaacctgac tctggcctcc agctccctga gtgattgcca tgcgccgctg
     7021 gtagaacaca ttcgccaggg cgtggttacc cgcatttata cctctggcct gcgcggtcca
     7081 ctggcggaag aaatctcccg tggtctgctg gcggaaccgg tgcaaatcca ctctcacggc
     7141 ggacgtgtgc atctggtaca gagcggcgaa ctgaatatag acgtggcttt cctcggcgtc
     7201 ccgtcctgtg atgaattcgg taatgccaac ggctacaccg gtaaagcctg ctgcggctcc
     7261 ctcggctatg caatggttga tgccgacaac gcgaaacagg ttgtgatgct cactgaagaa
     7321 ctgctgcctt atccgcataa tccggcaagc attgagcaag atcaggttga tctgatcgtc
     7381 aaagttgatc gcgttggcga tgccgcgaaa atcggcgctg gcgcgacccg tatgaccact
     7441 aacccgcgcg aactgcttat tgcccgtagc gctgcggatg tgattgtcaa ctctggctac
     7501 ttcaaagaag gtttctccat gcagaccggc accggcggcg catcgctggc ggtaacccgt
     7561 ttcctggaag acaaaatgcg tagccgcgat attcgcgccg acttcgccct tggcggtatt
     7621 accgcaacga tggttgatct gcacgaaaaa ggtctgatcc gcaaactgct ggatgtacag
     7681 agctttgaca gccacgctgc acaatcgctg gcccgtaacc ccaatcacat cgaaatcagc
     7741 gccaaccagt acgctaactg gggttcaaaa ggcgcatcgg ttgatcgtct cgacgtggtg
     7801 gtactgagcg cgctggaaat tgacacccag ttcaacgtta acgtgctgac cggctctgac
     7861 ggcgtactgc gtggtgcttc cggtggccac tgcgataccg cgattgcctc tgcgctttcc
     7921 atcatcgtcg cgccgctggt acgcggccgt attccgactc tggtggataa cgtgctgacc
     7981 tgcatcaccc cgggctccag tgtcgatatt ctggtcacag accacggtat cgcagttaac
     8041 ccggcacgtc cggagctggc agaacgtctg caggaagcgg gcattaaagt ggtttccatt
     8101 gagtggctgc gtgaacgtgc gcgtctgctg accggtgaac cacagccgat tgaattcacc
     8161 gaccgcgtcg ttgccgttgt gcgttaccgc gatggctcgg tgatcgatgt tgtgcatcag
     8221 gtgaaggaat aagccatgca cctgcttcct gaactcgcca gccaccatgc ggtgtcaatt
     8281 cccgagctgc tcgtcagccg ggatgaaagg caagcacggc aacacgcctg gctcaagcgc
     8341 catcctgttc cactggtctc ctttaccgtg gttgcgcctg ggccgattaa agacagcgag
     8401 gtcacacgcc gaatttttaa tcatggcgtg acagccttgc gtgccttagc cgcaaaacag
     8461 ggctggcaaa ttcaggagca ggttgcactg gtttccgcca gcgggccgga gggcatgttg
     8521 agcattgccg ccccggctcg cgacctcaag ctcgccacca ttgagcttga acatagtcat
     8581 cctctcgggc ggttatggga tatcgatgtc ctgacacccg aaggcgaaat tctctcccgc
     8641 cgcgactatt cactgccgcc tcgccgctgc ctgttgtgcg agcaaagcgc agctgtttgc
     8701 gcgcgtggaa aaacccatca actgaccgat ttactcaacc gcatggaggc gctgctgaac
     8761 gatgtcgatg cctgcaacgt caactaaaaa cacaaagctt gcgacgtcat taatcgatga
     8821 gtacgccctg ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg
     8881 cctcgtggat cggattaact gcggtgcgca taaagatatg gcgctggaag atttccaccg
     8941 cagcgcgctg gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc
     9001 tgaaatggca ccagaagagg tactccacgg attacgccca ataggtatgg cttgcgaagg
     9061 tgatatgttc cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg
     9121 gctactgtgt gcggcaattg gccgtttgct tcaactcaac cagtcgataa cgccaataac
     9181 aatttgtgcg acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa
     9241 taattcacaa ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg
     9301 cggtgaagcc gaagcgggtt atccactggt gatcaaccac gccctgccgc attacctcac
     9361 tctgctggat caggggttag atcctgaact ggcactgctc gataccttac tcctactgat
     9421 ggcgatcaac ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct
     9481 acagcgcgag gcgcaaacat tattgcaaaa agggggcatc cgaacccccg ccgatctcga
     9541 ttatctccgg cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc
     9601 cgacctgctg atccttactt ggtttttagc acagatttaa ttatttaagc acttgataaa
     9661 attggaatat taattgtcgg agaacccgta tgtctttagc aaaagataat atatggaaat
     9721 tattggcgcc cctggtggtg atgggtgtca tgtttcttat ccctgtacct gacggtatgc
     9781 cgccgcaggc gtggcattac tttgcagtgt ttgtggcaat gattgtcggc atgatcctcg
     9841 agccaattcc ggcaacggcg atcagtttta ttgcggttac tatttgcgtt attggcagta
     9901 attacctgct ctttgatgcc aaagaattag ctgacccagc gtttaatgcg caaaaacagg
     9961 cgctgaaatg gggtctggct ggtttttcca gcaccacggt atggctggta tttggcgcat
    10021 ttatttttgc attagggtat gaagtttccg ggttaggtcg tcgcattgcc cttttcctgg
    10081 tgaaattcat gggcaaacgc acgctgacgt tgggttatgc gattgtcatt atcgacattc
    10141 tgctggcacc gtttacaccg tccaacaccg cgcgtaccgg gggtacggtt tttccggtca
    10201 ttaaaaacct gccgccgctg tttaaatcat tcccgaacga tccgtccgcg cgtcgtattg
    10261 gcggctattt gatgtggatg atggtcatta gtaccagtct gagttcgtcc atgtttgtca
    10321 ccggtgcggc accaaacgta ctgggtctgg agttcgtcag caaaattgcc ggtatccaga
    10381 ttagctggtt gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg
    10441 cgccgtggct ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa
    10501 cctgggcggg tgatgaacta aaaaccatgg gtgcgctgac gcgcagagag tggacgctga
    10561 ttggtcttgt attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta
    10621 ctgcggtagg tctgctggca gtttcgctaa tgctggccct gcacgttgtg ccgtggaaag
    10681 acattacccg ctataacagc gcatggaaca cactggtcaa cctggcaact ctggttgtga
    10741 tggctaatgg cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc
    10801 acctggaagg attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg
    10861 cacactacct gtttgctagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg
    10921 ccgtcggtaa aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt
    10981 ctatcggtat catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg
    11041 gctgtggcta tgtgaaatca aaagattact ggcgacttgg tgcaatcttc ggggtgattt
    11101 acatctctat gctgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc
    11161 gccaaacgct gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat
    11221 gatttaatag atagtattca gtatatgtat gagttccaca cccattatga aagcattctg
    11281 gcgtaacgcc gcgttgctcg cggtttctct gcttcccttc tcttctgcca acgccgtggc
    11341 gttgcaggca aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac
    11401 cggattttgc cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca
    11461 aaccgaaacg accaacaaag ctgattttct gaccgtacat ggtctgtggc caggattgcc
    11521 taaatcggtt gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac
    11581 tcgcccaatc ccgaatctgc cagaagcacg tgccagccgg atgtgttcat cgccggaaac
    11641 cgggttatcg ctggaaacgg cagctaaact aagtgaagtc atgccaggag ctggcggacg
    11701 ttcctgcctg gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccaga
    11761 cgcatacttc ggcacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa
    11821 attccttgcg gataactacg gtaacacagt gagccgccgt gactttgatg ccgcctttgc
    11881 caaaagctgg ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc
    11941 gtatttgact gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc
    12001 caactcattc ttgccacaac cacacccagg caactgtggc aaaacctttg tgattgataa
    12061 agcaggttat taatcctctg atttacaaaa cacgctaatt cgtcttccat aaaccatgac
    12121 gattaagatt tatacataat gcgctacatt gaggaattcc ctgaatgtga cgcaaatcac
    12181 ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt
    12241 gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa
    12301 aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc
    12361 tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc
    12421 gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg
    12481 ttaaattccg caatcttagc gatggcgaag tgcgtgtgcg cacgctagtg tatccggcaa
    12541 aaatgaccga cagcaatact cagctttccg ttatggctcc ggtaggtgct gcactgctgg
    12601 ggctgcgcgt tggcgattcc attcactggg aacttccggg cggcgttgca actcaccttg
    12661 aagtgctgga actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc
    12721 tgcacaagcg ataactcgct ttgcccagag gatgcactcg catcctcttt ccgctcaaat
    12781 atcaaccaga tttatcattt tgctcttaag cgcagcattc taatctgaac tggttattca
    12841 tctgcggaac ctcatatgag cgaagctctc ggtatcactg cattttatat attcatagca
    12901 gcaattattg ttgctgcagt gctatacctt gaacaacgct ggtagcgcct ggttttattt
    12961 attttccagc tcaatagcaa attcagatca aatcagcctc gcaaatcata gattcataac
    13021 caataatgtg ctgttatgta tacatgcaaa aacaaggaga gaatgcttat gtacaaaaca
    13081 atcatcatgc cagtggatgt tttcgaaatg gaattgagcg acaaagcggt tcgccacgct
    13141 gaattcctcg cccaggatga cggagttatt catctacttc acgtactacc ggggtcagcc
    13201 agcctgagcc tgcaccgttt tgccgctgat gtgcgtcgtt ttgaagagca tctgcaacat
    13261 gaagcagaag aacgtctgca aacgatggtc agccacttca ccatcgatcc ttcccgcatt
    13321 aagcaacatg tccgttttgg tagcgtgcgg gatgaagtca atgagttggc agaagaactg
    13381 ggggctgatg ttgtagttat cggttctcgc aacccatcga tttcgaccca tctgttaggt
    13441 tctaacgcct cgagcgtgat ccgccacgcc aatctgccgg tgctggttgt gcgttaattg
    13501 ccagaataaa tatcccgccc tgcccggagc catcccggcg gggcttttta ttgccataaa
    13561 aaagccgcca ggtttgaccc cggcggctaa gcaattgcag gtgaatctta cttcttctta
    13621 tgctgttttg gtgcgaatca ggtagtcaaa agcactcaga gaggctttgg caccttcgcc
    13681 agtggcgatg atgatctgct tgtacggaac cgttgtacag tcacccgctg cgaacacgcc
    13741 tttcacgttg gtttcgcatt tcgcatcaat gataatctca cccatgcggt tacgtccgac
    13801 tgcgccttcc agccagttgg tgttcggcag cagaccaatc tggacgaaaa taccggccag
    13861 ttcgatgttg tgaatatcgc cgctgacacg atcgcgatat tccagaccaa cgactttgct
    13921 gccgtcgcct ttcacttccg tggtttgcgc attcagaata atttcgacgt ttttcaggct
    13981 gcgcagtttg tcctgcagaa cctggtcggc tttcatttct ggcgcaaatt ccagcagcgt
    14041 tacgtgctca acgatacccg ccaggtcgat tgccgcttcc acgccggagt taccgccgcc
    14101 gataaccgct acgcgtttgc ctttaaacag cgggccgtcg cagtgcgggc agtaggtcac
    14161 gcctttggtg cgatactgat cttcgcccgg aacgttcatg ttgcgccatt ttgcaccggt
    14221 cgccacgata atgctgcgtg ctttcagtac cgcgccagaa gctgtttcaa tctgatgcag
    14281 gccgccttca accgctgccg ggatcagttt gctggcgctc tggctgtcga tcacatcaac
    14341 gtcgtattca tcaacgtgaa ctttcagcgc acctgccagt ttctggcctt cggtcttcgg
    14401 tacagaaatg tagttttcga tatcaacggt atcgaggatc tgaccaccaa aacgttcgcc
    14461 catcagaccg gtacggatgc ctttacgtgc ggagtaaatt gctgccgctg cgcccgccgg
    14521 gccggaaccg acgattaata cgtcataagc atcacgcttg ttcagctctt ctgccgcacg
    14581 tttttccgcg ccagtatcaa ttttggcgac gatttcagtc aacgtcatgc gaccctgacc
    14641 aaactctttc ccgtttacga acactgccgg aacgcccatc acgttgcgat cggtgatttc
    14701 gttctggaag gtgccgccgt caattgcagt gtgcttgatg cgcgggttta gtacgctcat
    14761 caggttcagc gcctgcacca catccgggca gttgtggcaa gagagcgagt aataggtttc
    14821 gaattcaaaa tcaccgtcaa tatggcgaat ctgctccagc agagactgcg cttctttcga
    14881 cggatgacca cctgtccaca gcaacgccag taccagcgag gtaaactcgt ggcccagcgg
    14941 ggagcctgca aaacgtggcc cctggttgga acctgggttg gtgatcagga aagacggctt
    15001 acgcactggc aagctgttat cttctttaaa ggtgactttg tctgacagtt ctgcgatttc
    15061 agccaacagt tccttgattt ctgccgattt agcgctgtca tccagcgtgg caattaactc
    15121 aacaggcttg gtcaatttct caaggtaagc cttgagttga gttttcatat ttgtgtcgag
    15181 catgtttatc tcctgggctt taaacatcat catgcaagcg gcctctgcgg ctacctggat
    15241 gcagcttgca tcatggtgcg gggaagaaaa tcgggtgccg cagcacccga agaattaggt
    15301 gaatttccga agtatttcgc gccggagttg gtagcgcgaa agacgaagga aatttagatt
    15361 ttaccaacca ggtccagaga cggagccaga gttgcttcac cttctttcca tttagccggg
    15421 caaacttcac ctgggtgaga agctacgtac tgtgctgctt tgattttacg cagcaggtca
    15481 gacgcgtcac ggccaatgcc ttcagcggta acttcgattg cctggatgat accctgcggg
    15541 tcaacaacga aggtcgcacg gtcagccaga ccttcatctt cacgcatgtt gtcgaagtta
    15601 cgggtcaggg cgccagtcgg gtcgccgatc atcgcatatt tgattttagc gatggtttca
    15661 gagctgctgt gccatgcttt gtgggtgaag tgagtatcgg tagatactgc gtatacgtct
    15721 acgcccagtt tctgcagttc ttcgtagtgg tcagcaacgt cacccagttc ggtcgggcat
    15781 acgaaagtaa agtcagccgg gtagaagaag aagacgctcc agcggccttc ggtatctttt
    15841 tcggtgattt cgatgaattc gccgtttttg aatgcctggt ttttaaaagg tttaattttg
    15901 gtgttaatca aggacatcta tacttcctcc gtgttttcga tgagatgtaa ggtaaccaat
    15961 ttttgccgat tcggctaatg cgtttccatt atcaaatcga taagttttac cttacaacct
    16021 tcgtaagaca actttgtgaa ctttgcaccg ccagcggcga taaaaagtaa aatggccccc
    16081 tttccaggtg accgtgtaat ctgaactacc ctttggtatc ttcagcgcct gcctgcaggc
    16141 tgaggtgttg cccttacaaa tgcaacaacg acatggatta caacaccctc ataaacaaag
    16201 ggcaatcacc tgatctaagc tcttacctat gacagtgata ggttatgcct tttacccgac
    16261 ttttgcactg actgaaaagg acaaattaat gttaaaaaag atacttttac tggctctgct
    16321 tcctgcaatc gccttcgcag aggaacttcc tgatccagta aaagcgattg aaaaacaggg
    16381 cattacaatc atcaaaacgt tcgatgctcc cggaggaatg aaaggttatc tcggcaagta
    16441 tcaggatatg ggcgtcacca tctacctgac tccagatggt aagcacgcta tctccggtta
    16501 catgtacaac gagaaaggtg aaaacctgag taacacactt atcgaaaaag aaatttacgc
    16561 accagccggg cgcgaaatgt ggcaacggat ggaacaatcc cactggctcc tcgacggtaa
    16621 aaaagacgcg ccggtcattg tctacgtctt cgccgatccg ttctgcccat attgtaaaca
    16681 gttctggcag caggctcgcc cgtgggttga ttctggcaaa gtgcaattaa gaacattgtt
    16741 ggttggcgtt atcaagccag aaagcccggc gacaacagcg gcaattcttg cctccaaaga
    16801 tcccgcaaaa acctggcaag aatatgaagc ctctggtggc aagcttaagc taaacgtgcc
    16861 tgcaaacgta agtacagagc aaatgaaagt gttaagcgac aacgagaaac tgatggacga
    16921 tctgggggca aatgtcacgc cagctatcta ttacatgagt aaggaaaata cactgcaaca
    16981 ggccgtgggg ttgcccgatc agaaaacgct taatatcatt atggggaata aataaaagat
    17041 aactcatgga tggttgaaat atatctctgc ttttcaacca tcctaaaaaa gcatgaattg
    17101 ctcgctctgc cctaagtggc tgtgctattt tagaaaaact taattctgag ttaagcaaaa
    17161 aaacataata caaataaata caaaagcaac attattgaac aaatgaatga tttatagaag
    17221 agataatata acggagtatg ttatggcgaa tctttatgac ctaaaaaaat ttgatctgaa
    17281 tttactggtc atatttgagt gtatttatca acatctcagc attagtaaat gcggcagaat
    17341 cattatatat aacgccctct gctgttagtc agtcgttaca acgtttacgt gcgcaattta
    17401 atgacccgtt attcattcgc tcagggaaag gtatcgcgcc gactacgaca gggttaaatc
    17461 tgcatcatca tcttgagaaa aatctcagag gcattgaaca aacgatcaat attgttaata
    17521 agtctgagct aaagaaaaat tttattatct acggtcctca gcttatttct tgtactaata
    17581 acaacatgct aatccgctgt cttagacaag atacttcaat cgatatagaa tgccatgaca
    17641 tactgctttc agctgaaaat gccgaagaac tgttggttca acgcaaaact gacctggcaa
    17701 ttacactaca accagtaatt agccgctctg tcatctgcat gccgttacat actattcgca
    17761 attctcttat atgtagcaac aaacatcctc gtattaccga taccagtaac tatgaacaaa
    17821 ttatcgccga agaattcacc ttgttgatat ctaaatcagc agggattgac gaaattcaaa
    17881 tggacataga tgaacgtttt atgaaccgta aagtttcatt tcgcagttct tctttgttga
    17941 cgataattaa tagtatttct gttactgact tattgggcat tgtgcccacc gaactgtacg
    18001 atttacatcg tgattttttg aagttacaag agataaaact ggaacgacca cttcctgcgg
    18061 ttaaacttta tatttcctat aacaaggctt cgttgaacaa ccttgtcttc tcacgattca
    18121 tagatcgttt aaatgatagc ttctgattta ttaccacgga taaatgttat attacgcatc
    18181 aattaattca ttgtgtgaat ataaaataat gtgctctatc ttgcttcgtt taccctataa
    18241 ttaacggatt catctcacga gtcaccccgt aacgttcaga gagcgttttc agatgtctat
    18301 ttataaaatc ccgcttccac tgaatattct ggaagctgca aaagaacgta ttacctggac
    18361 acttaacacc ctaccccgcg tctgcgtttc tttttcgggt ggtaaagatt ccggattaat
    18421 gctgcatctg accgccgaac ttgcccgaca aatgggcaaa aaaatttgtg ttttatttat
    18481 cgactgggaa gctcagttct cttgcaccat taactatgtt cagtctctgc gcgagttgta
    18541 caccgatgtc atcgaagagt tttactggat tgcgctcccg cttacaacgc aaaattccct
    18601 atcacaatac caacccgaat ggcagtgctg ggaacctgat gtcgaatggg tacgtcagcc
    18661 cccgcaagat gcgataaccg atcctgactt tttctccttt taccagcctg gcatgacctt
    18721 cgaacaattt gtgcgtgagt ttgccgaatg gttttcgcaa aaacgtccgg cggcgatgat
    18781 gatcggcatc cgtgcggatg agtcctacaa ccgttttgtc gccatcgcca gtttaaataa
    18841 acaacgtttt gccgatgata agccctggac tactgccgca ccaggcggtc atagctggta
    18901 catttacccc atctacgact ggaaagtcgc tgatatctgg acctggtatg ctagccatca
    18961 acgcctctgt aacccactgt ataatataat gtatcaggca ggcgttcctc tacgtcatat
    19021 gcgaatttgc gaaccttttg gtccggaaca acggcaagga ttatggctct atcacgttat
    19081 cgaaccggat cgctgggctg ctatgtgcgc acgagtcagc ggggtaaaaa gtggcggcat
    19141 ttacgccgga catgacaacc atttttatgg tcatcggaaa atcctcaagc ccgaacattt
    19201 agactggcag gagtatgcat tattgctact caacagcatg ccggagaaaa cagctgagca
    19261 ttatcgcaat aaaatcgcca tttatttgca ctggtatcag aaaaaaggca tcgaggttcc
    19321 acaaacccag caaggggaca ttggcgcgaa agatatcccc tcctggcggc gaatatgcaa
    19381 agttttactc aataacgatt actggtgtcg ggcattatca tttagcccga ccaaagcgaa
    19441 gaactatcag cgttataacg aacgaataaa aggaaaacgt caggaatggg ggatactatg
    19501 caacaacgat taacgcagga tttaacccaa tttctcgcca gcttgccgga agacgatcgc
    19561 atcaaagcga ttaatgaaat ccgcatggcg atccatcagg ttagcccctt tcgagaagag
    19621 ccagtggatt gcgttctgtg ggttaaaaac agccaactta tgcctaatga ttacaaccca
    19681 aacaacgtgg caccacccga aaaaaaactg ctgaaaaaat ccattgaaat agatggtttt
    19741 acccaaccca ttgtggtcac gcatacggat aaaaatgccc tggaaatcgt ggatggtttt
    19801 caccgccatg aaattggtaa aggctcatca tcgttaaaat tacgcttaaa aggttatttg
    19861 ccggtgacct gtctggaagg tacgcgtaat caacgcatcg ccgcgactat tcgtcataac
    19921 cgcgctcgtg gacgtcacca aatcactgcc atgtcagaaa tcgtccgtga actcagccag
    19981 ttgggatggg acgataataa aattggcaaa gagctgggaa tggacagcga cgaagtgcta
    20041 cgcctgaagc aaattaacgg cctgcaagag ttgtttgccg accgtcaata ttcccgcgcc
    20101 tggacggtta aatagctaaa gctggcgcag gcgttcagct gctgccagca acgtcgattc
    20161 cttcttggca aaacagagac gaatcagttt atgtgggaag ggatcggcgc aaaacaccga
    20221 tagcggaatc gccgctacgc cgtgctcctg cgtcagccac tggcaaaact caacgtcatc
    20281 cagggtagat acggcgctgt aatccaccag caaaaagtat gtaccttcgc acggtaaaat
    20341 ctccagccgg ctttcattta aggcattcac cagaatatcg cgcttctggc gataaaagtc
    20401 cggtaacgca agataatgct caggttctgc acgtagcata tcggcaagcg ccagctgtgc
    20461 cggggtattc accgaaaagg tcagatactg atgtaccttg cgaatttcgg cactgatggg
    20521 cgctggcgca acgcaataac ccactttcca gccggtcata tgataggtct tgccaaacga
    20581 tgacaccgcc accgcccgct cacgcaactg cggatgcgcc tgcacgctgg catggccctg
    20641 ttgtgaaaag ttgatgtgct cgtaaacttc atcgctaatg acaaaaatct cgtgcccggc
    20701 aattgcctgc cacaaagcgg cgaaatcagc ctgctgccag acagttgcac tggggttatg
    20761 cggggtgttg aggatcacca gtcgggtgcg ctcgcttaac aatgcggcaa attcctgcca
    20821 gtccacgcga aaatgcggtg gttgcagtgc aatacgtttc actattcccc ccgaaagcgc
    20881 gatggcgggc gcgtaactgt catagctggg atcaaagcag atcacttcat caccattgcg
    20941 caccaatgcg gtaatcgctg catataacgc ttccgtcgcc cctgccgtta cggtgatatc
    21001 actatcggca tccggttgat agccatacag acgttccgtt ttctgagcaa tcgcctcgcg
    21061 caaggcctgc acgccagtca taggcgcgta ttggtttgcc ccctggtcaa catggtacgc
    21121 cagccgctcc tgcaaatagc gcggaccatc aaaatcagga aagccttgcg aaaggttaat
    21181 cgcctggtgt tgctgcgcca gcgcgctcat ctgggtgaaa atagtggtgc caagttgtgg
    21241 aagtttgctt tgtggaatca gagggttatt tgtcattttt tattgtgcct gacgaagttg
    21301 tgttgaaggc actataacac gatgctagta tttggcaatc aagacgttta gatgtctaaa
    21361 taaaacaata aggacaacac aacatgcctc acaatcctat ccgcgtggtc gtcggcccgg
    21421 ctaactactt ttcacatccc ggcagtctca atcacctgca cgattttttc actgatgaac
    21481 aactttctcg cgcggtgtgg atctacggcg aacgcgccat tgctgcggcg caaacaaaac
    21541 ttccgccagc gtttgaactg ccaggggcaa agcatatttt gtttcgcggc cattgcagcg
    21601 aaagcgatgt acaacaactg gcggctgagt ccggtgacga tcgcagcgtg gtgattggcg
    21661 tcggcggcgg cgcactgctc gacaccgcga aagccctcgc ccgccgtctc ggtctgccgt
    21721 ttgttgccgt tccgacgatc gccgccacct gcgccgcctg gacaccgctc tccgtctggt
    21781 ataacgatgc cggacaggcg ctgcattatg agattttcga cgacgccaat tttatggtgc
    21841 tggtggagcc agagattatc ctcaatgcgc cgcaagaata tctgctggcg gggatcggtg
    21901 acacgctggc gaaatggtat gaagcggtgg tgctggcccc gcaaccagaa acgttgccgc
    21961 taaccgtgcg actggggatc aataatgcgc aggccattcg cgacgtcttg ttaaacagta
    22021 gcgaacaggc gcttgccgat cagcaaaatc accagttaac gcaatcattt tgcgatgtgg
    22081 tggatgcgat tatcgccgga ggtggaatgg tcggtggtct gggcgatcgt tttacgcgtg
    22141 tggcggcggc ccatgccgtg cataacggtc tgaccgtgct gccgcaaacc gagaagtttc
    22201 ttcacggcac caaagtcgcc tacggaattc tggtgcaaag cgccttgctg ggtcaggatg
    22261 atgtgctggc gcaattaaca gaggcgtatc agcgttttca tctgccgact acgctggcgg
    22321 agctggaagt ggatatcaat aatcaggcgg agatcgacaa agtgattgcc cacaccttgc
    22381 gcccggtgga atccattcat tacctgccgg tcacgctgac accggatacg ttgcgtgcag
    22441 cgttcgaaaa agtggaatcg tttaaagcct gacgtacaca tttagcagca gcgcgcgccg
    22501 ccttttccac cgtagcgcgc gtcctgccgc tcacggaaaa actcctcgta ggtcatcggc
    22561 gtttgatcgg gatggttaac ccgcatatgt tcgacatagt tgtcgtaatc aggcatacca
    22621 atcatcagct tcgccgcctg acctaaatat tttccggctt ttgccagtga atcaaacatc
    22681 taaacctccg gtaatacaaa tgccctcctg aattaagagg gcttaattgc tatcaacacg
    22741 ctcgggctga gagtaagggg aacgctcagc acatcccccc tcactctgac tttagtgcgc
    22801 gcctttcgcc tgcgccacga tctcctcgac attttccggc attggctcat acggcgtttc
    22861 tttcgccgtt ggcttcggct ctttcaatgc cgccagtgcc gtcttaatcg agaacagcgc
    22921 cagaaccacc acgaccacca taaagaagat ggttaacccg gcatccagac ggttgttgaa
    22981 taccagttgc gccagttgtg actcagtata ctgcgacgga atattgccgc tgtcgatcat
    23041 tgcctggaac ttgttagcaa tcgccaggaa gccgactttc gcatccgggc taaacgcttt
    23101 ctgccagcct gcggtcagag tacaaatcag cagccaggcc gttggtacca gcgccaccca
    23161 ggcgtaacgt tgacgtttca ttttgaacaa cactacggca cagagcatca gcgccatccc
    23221 tgccagcatc tggttggcaa taccaaacag cggccacaag gtgttaatac cgcccagcgg
    23281 atcgaccacg ccctgatgca ggaagtagcc ccacgccagc acgcacagcg ccgttgccag
    23341 caggttagca ggcagtgaat cggtccgttt caggcccgga gacaccacgc ccagcagatc
    23401 ctgcaacata aagcgcgcag cacgcgtacc tgcatccacc gccgtcagaa taaacagtgc
    23461 ttcaaacaaa atggcgaagt gataccagaa cgccacatcc atcatgccgc ccagcgcgcc
    23521 gtgcagaatg taggccatcc ccaccgccag cgtcggcgca ccgcccgcac gggaaatgat
    23581 cgactgttca cccacttcgc tggcaatctg gttgagcgta tctggtgtaa tgctaaatcc
    23641 ccagctactc accacttgcg ctgcagaagc gaccacatcc gccgtacctg ccggagccag
    23701 caccgccatc gggctgttca tagcgaagta cacacccgga tcgatgatac aggcggaaac
    23761 cagcgccata atcgctacga aggattccat taacatcccg ccgtagccga taaaacacgc
    23821 ctgcccttcg ttcgccagca tcttcggcgt ggtaccggaa gagatcagcg catggaagcc
    23881 agacaccgcg ccacaagcga tggtgataaa caggaacggg aacaggttac cggtccatac
    23941 cgggccagtg ccatcgacaa atttagttag cgcaggcatg gtcagcgtcg ggcgcataat
    24001 caaaatgcct accgccagac cgacgatggt cccgattttc aggaaggttg agaggtagtc
    24061 gcgcggggcc agcaacagcc acaccggcag caccgccgcc acaaaaccgt aacccaccag
    24121 catccaggtc agctgcacgc cggtaaagtc aaagtacggt gcccaggtcg gactttctgc
    24181 cacccagccg ccagagataa tggcgaaaat caggaacacc aggccgatga ccgacacttc
    24241 gccaatacgc cccggacgca gatagcgcag gtagatcccc ataaacagcg ccagcggaat
    24301 ggtgaacgca acggtatacg ttccccacgg gctatgggtc agggctttca ccacgatcat
    24361 cgccagcacg gcaaggataa tgaccatgat cataaagcag gccaccagcg caatcacccc
    24421 ggcggttggc cccatctcct ctttgaccag ctcacccagc gagcgaccat cgcggcgcgt
    24481 ggaaacaaac agcaccatga aatcctgcac cgcaccggca agaaccaccc cggcaagcag
    24541 ccagatcatc cctggcaggt aacccatttg cgccgccagt accggcccca ccagcgggcc
    24601 agctccggca atggccgcaa aatggtgacc gaacagcact ttcttgtccg tcggcacata
    24661 gtccagcccg tcgttatggc gcacagctgg cgtcatacgc gtcgggtcaa ctgccagcac
    24721 atttttggcg atatacaggc cataaaaacg gtaagcgatc agatagatac agaccgacgc
    24781 caccacaatc cacagcgcgt tgatctgttc cccacgattt aaagcaatgt atcccagagc
    24841 aaatgctccc attacagaga gcactgtcca gacgaggtat ttccctgatt tgttcatagt
    24901 tgttatccgt gtgcgtgtcc atagagatgt tacattttgt ttctataaca tctttgtgca
    24961 atttaacaac acagcaacca tgtaaatcgg agttgaaacc aatatttaac ttaacaatgt
    25021 taactcgatc actccgttaa ccgagttttc ctgcaatctc atttaattct gtcgggctca
    25081 tcccaaaact gccgtaccca gccgacaggt gcagcaacgc cgcccctgtt catcgaaaac
    25141 gacgatttcc cagctctgat tttgccgccc aagatgcagc ggctggcaga caccgcgtac
    25201 ctttccctca gacaccgggc gatggtgtgt tgcattaagt tctgtgccca ccacacactg
    25261 tccgtcgcga gtcatcataa atccggccat cgatcccagc gtttccgcca gcgccgccga
    25321 cgcgccgcca tgcagcaggc caaacggctg atgagtacgg gtatcaaccg gcatttcggc
    25381 ttccagcaca tcatcgtcca gacgggtgta aacgattccc agatgcgcca ccattgtgtt
    25441 atcgctggtg gcgttcagct cgtcgagcgt taaatggcgt ttccagatca tgcttatgcc
    25501 cccagcgttg agccgccatc gaccacaata tcctgtaggg tgatatggct ggcgagatca
    25561 gaggcgagga acaaaatcgt attggcgatc tcctgtggac gggcgatttt ccccagcgga
    25621 atgccgagtt taaactgctc gccaaagccg cgaatacgct gctcttcggc gtcatcgctt
    25681 acccacagcg tgcgttgcat atcggtgtcg gtggagcctg gcgaaaccac attacagcgc
    25741 accccgctac ccgccagttc cagcccgacg ctcaatgcca ggcttttcag cgccgctttc
    25801 gacgcgccat aagcactcat accaatgcgc ggcgtgtgtg cggcgtcgga cgcaacagta
    25861 acaatcgccc cgccccgctg acggcgaaac tggttcatgg tctgctggaa caggttaaac
    25921 gcaccgccga cattgaccgc aaaagtctgc tgccagtcct ccttactgag ctgatcggtc
    25981 gcgcccatgc gcaaaattcc cgccgcattg accagcacgt ccagtcgctc cgtttcagct
    26041 aacagtcgct gacatacctg cgccacctgc gcagcatcgg caacatccat cacttcggtc
    26101 gcaaagggat attgctcctg agcgaacgct tgatcaaaac cagtgacttt cgctcccgcc
    26161 tcaacaaacg ccagcgccgt ggcgtagccg atacctttcc ctgcgccagt tacccagaca
    26221 tttttaccgc tgaaatccat tatttcacct cgcgggaaag tagcttccac caggcgtcga
    26281 tggtcgggtt tttcgccagc atgacaaagt cgatatcgcc atgcactttg cgccagcgcg
    26341 ccgccagcgc catcatgcgc accgaatcca gaccgtagtc gatcaggttg tcgtcatcga
    26401 acggttcatc ggactcgtcc agcaacggca ggatcacctc acgcagcgcc gctttgctgg
    26461 cggggatagg tgctggcagt aattcttcgg tcatcaccac ccggccagaa cgtccggcca
    26521 catatttcag cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca tctgccacca
    26581 taaacggttt aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag ccaatgtggg
    26641 catatacccc ggtaataatc agctggttac gtccactctc tttcagcatt tgctccagcg
    26701 gggaacgatg aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg gcatctggcg
    26761 tcaggcgatc caccaccttt tgctgttccg gcgaacgggt caggccaggc ccccacatat
    26821 cattcaacag cgccctatct tcatcgcttt gctctttcgg ctgggcggtg taataaaccg
    26881 ggatattgtg ctgtttgcag tagtcgcgta gtgccgcaat attcgcgatc acctgctcca
    26941 tcatcgggca gttctcgccc cagaagctga caaaatagtc ctgcatatca tggattaaca
    27001 acgcggcacg ttgcggttca aaggcccagt cgactttatt ctgcggaata tcgtgagact
    27061 ccggcagtgc gtaagcctgt aattttggaa tagccatcgt gttctctcct tcaggctgat
    27121 gcgcgtgacg ccagccactg acgtaattgt tttttatcga ctttcccgac cgccgtcagc
    27181 ggaagtgaat ccacacactc cacgcgatcc ggtaatttaa attcggcaat accctgttca
    27241 cgcaggaaac gacgcacctg caccgcgcgt agaggctctt ttaccaccag ataagcacag
    27301 cttttttcgc ccatcagctc atcttccatg ctcaccagtg cggcgtagat caccgccgga
    27361 tggcgcagca gcaggttttc gatctcttcg gcagcgatct tctcgccgcc acggttgatc
    27421 tgatctttct cgcgcccctg cacggtgatg tacccctctg gatcaataga gatcagatcg
    27481 ccggaacagt aaaaaccgtt ggcatcaaag gcgctggcat tgtgctgcgg gcttttgtaa
    27541 tagccacgga aggtgtacgg accgcgcgtc atcaggcgtc caacttctcc ttgcggtagt
    27601 ggatttcctt cagcatcggc aacccacact tcatcgtccg gacacattgg atagccctgg
    27661 gtatggataa ttttctccgc gctatcatca agacgggtgt agttcaccag cccttccgcc
    27721 atgccaaaca cctgctgcaa ctggcagcca atctcagcgg gaatacgcgc cgcaagcgtg
    27781 gcagaaagac gtgcgccgcc gacctgtaac agttttagcg aggcaagctg cgcctggctt
    27841 tcgccttcgg tcagcgcctg taaccacagg ctgactgcgg gtggcaccag cgcggtgacg
    27901 ttcacctgat gtttttcaat caatgggaaa cagagcgtgg cgctgggatc ggccgccaga
    27961 acgaccgttc ctccggcaag aaagacgccc agcgatcccg gcgaactcat ggcgtagttg
    28021 tgcgccgccg gaatcgcgca taaatagcgc gtctgttgtg tgaactgaca aatctcgacg
    28081 ctacgacgca cgctgtagta gtagtcgtta tgagtgcgcg ggatcagctt cggtgtgccg
    28141 gtggtgccgc cggaaagctg gaaataggcc acttcatcag caggtgacgg cgtggcagta
    28201 aaatcctcag ccggatggtt aatcgcatcc tgcaagttat gctcaccgct gtcgttgtgc
    28261 agttgcacca cgcgaatgga ggaatgctct gcaacaaatg tattgaggaa atcatctccg
    28321 ctaaacagcg catgctggcg atcggcaatc agtaacgccg gttcaatctg gctggcatag
    28381 gcgttcagtt cactacgctg atggctgaac agcgccagca ccggcgcaac gcccagtttc
    28441 agcagcgcga aaaaagtaat gtaaagttca gcgacgttac ccagttgtac cagcgcagtt
    28501 tcaccaggtt taatgccctg acggcgcaaa ctacacgcga ggttatccgc cgcctgattc
    28561 agctcccggt aactcaactg ccgctcgccg tcgatgaccg cgatgctatc gcttgcggca
    28621 tgacgggtca gaatgtcggt cagcggcaaa tcctgccagt agcctttttc ccgatagcga
    28681 cgggcaaact cttccggcca gcgggtgaat ggaatgctca tcctcactcc ttaatgcaat
    28741 ccaaaaacgt tcaacatggt agaaagtttg acgcccgttt cgcgccactc acccaacggt
    28801 gacgacgcag gcacaatccc cgctccggca aacagacgca cctgattttc ccgcagcttc
    28861 gcgcagcgga tggtcaccac ccactcgccg ttaccttcgc tgtcacacca acccacaatg
    28921 ccgccaaaca gttcgcggtc gaacggctcc agttcagcaa taacctgggt cgcggcctga
    28981 tgcgggaaac cgctcagcgc gggggtcgga tgcagtagac aggccagagt cagtgcgttt
    29041 tcttgcgaat tcgctttacc ttcaaaggga gttgcgagat gccacagcgt cggcgtggtg
    29101 atcagttgtg gagaagaagg aacgtgtaac tcactactgc gttcgcgcaa tacctctttc
    29161 atcgcctgag tcaccagttc atgttcatgg cgatcttttt ctgacgccag cagacgatta
    29221 cctgcttcgc gatcgagcac ttcatccggc tgacgacgcg cggaaccggc taacggaatg
    29281 gagctaaaac gctcgccgtc tttacgtagc agcagttccg ggctggcccc cagcagaacg
    29341 ccaccatcag ccagcgggac atggaagttg taactaaccg ggttttgcgc aatcaaccgt
    29401 tccagcaata cgccactatc aatggcggcg tcagtggtga tatcaatcaa ccgtgacaac
    29461 accactttgt cgacctgcgg cgtggcggta agtgcggcgg cgcgagcaac catctgttca
    29521 aacgtggttt gatccggaat tgcctgacgt tccaccacgt tcagcgactg gctgcgggtg
    29581 aaacggcgtg cggaagcttg tttttcctgg cgggagaacg actgccagga ttcaggaata
    29641 tacagcgatg aaggctgacg tggatcgaag ggaatcgccc cgaccatcac cggatttttg
    29701 atgccctgcg ctttggcatc ggcaaacagc gcggcgagtt tttgctggaa gggactgtcg
    29761 ggcgaatctc cgttcacagc gggttcatcg aagcgggcga aacatcctga cgtcgtaaaa
    29821 ctgcggtacg gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat ggtctgctgt
    29881 acttcctcag ccagtgacgt atccatatca tcctccacaa aatgataaag gctttaataa
    29941 tgattatcat ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta tgtcaactct
    30001 tgaggtaacg caatctgcaa ctcggggttg catctggttg tggttacaat gaaaatgaga
    30061 agcattaact tcattaataa caggaagctg acttgtgaga ctcgccccgc tctaccgcaa
    30121 cgcccttcta ttaacaggac ttttgctttc aggaatagtc gcagttcagg ccgccgactg
    30181 gccgcgtcag attactgaca gccggggcac tcataccctg gaaagccagc cgcagcgtat
    30241 tgtttccacc agcgtcaccc tgaccggctc actgctggcg attaatgctc cggtgattgc
    30301 cagcggcgcg accacgccga ataaccgcgt cgcggatgac cagggctttt tacgccagtg
    30361 gagcaaggtg gcgaaagaac gcaaactgca acggctctat atcggcgaac cgagcgccga
    30421 agccgttgcc gcgcaaatgc cggatctgat tttaattagc gcaactggcg gggattcggc
    30481 gctggcgctg tacgatcagc tttctaccat cgccccaaca ttaatcatca attacgacga
    30541 caaaagctgg caggcgctgt taacgcaact tggcgaaatt accgggcatg agaaacaagc
    30601 ggcagagcgg attgcacagt ttgataagca actggcggcg gcgaaagagc aaatcaaatt
    30661 accgccgcag ccggtcactg ccattgtcta taccgccgct gcacacagtg ccaatctctg
    30721 gacgcctgaa tcagcacaag ggcagatgct ggaacaactc ggctttacgt tggcgaaatt
    30781 gcccgcaggg ttaaacgcca gccaaagcca gggcaaacgt catgatatcg ttcagcttgg
    30841 tggggaaaat ctggctgcag ggttaaatgg cgagtcacta ttcctgttcg ccggtgatca
    30901 gaaagatgcc gatgctattt atgctaatcc gctgctcgcg cacctgcctg cagtacaaaa
    30961 caagcaggtt tatgcgctgg gaaccgagac gttccgtctg gattactaca gcgccatgca
    31021 agtgctggaa cggcttaagg cgctgtttta agcattaact gtcggacgct gtcacctgcg
    31081 gcggcgtctg gcgaaaacgc cgcaactcca ccagcaccag caataacaac acgccgataa
    31141 tcaacaaacc aaaaccgctc gcgcttgcgg aagcaaccgg tgtcatcatc gcgcccaaac
    31201 cgcccagcag cgccgcgcct atggcatcgc ccgtcacgtt ttgtgccgtc cacaaaccgt
    31261 taatccgccc taacatcgct tccggggttt gcgtttgcag cattgtatat tgcagcaacg
    31321 aactaaccgc gctcagccag ccgaacagcg ccagacaaac cacgcctaaa atccacatcg
    31381 gcatcaggcc aaacaaacca atggcgagga acgatcccag cgtggaaagc agcatcaata
    31441 accctggtcg cgcgctatgt gccagcttac cgctggttaa cgcgccaata gccgcgccga
    31501 gcgggatcgc cgcatagaga aaaccaatct gtgccgccga catctgccag ttgtcagcca
    31561 gcgccggata cagtacccgc accgcgctcg ccatcgtcaa taaaccaccc aacagcgcaa
    31621 tccctcctac cagcgggctg gcgagcagaa aacgaaatcc tgccagtaat gatttcaacg
    31681 gatgctcacg cggctgcggt ggcggtggca acgccggaag gcttaataac ggcagcaagg
    31741 taataaacgt gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca
    31801 ataaaccgcc aatcataggc gaaatcaccg accccagacg cacggtcaac atggtgatcg
    31861 ccccggcctg cattaagttt tcacgcccaa ccagtgcagg tgtcgccgcc agtagcgccg
    31921 taacgccaag cgatgcgaaa aaaccatccc ataaaccgag taaatagatt gccaatagag
    31981 atggctccgg cagcagcgcg ttcaggcaca gtccaatgaa gccaatgcca caggtgccgc
    32041 gcgccagcaa aatcactttt ttgcgctcat agcgatccgc cagcacaccg ccgaccatca
    32101 ggccaacaaa catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat
    32161 gcgtcatcat ctggatctgc accggtaccg cgacgccgag caaacccaga gacacaattg
    32221 agataaaacg agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt
    32281 taagcagcca ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta
    32341 ttcttaggct gcacatgcta acatatccaa ataagatcga taacgataat taatttcatt
    32401 atcatggaag ttcgtatgtc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga
    32461 ttgctgttat tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc
    32521 cccgcttccg tagtgctgga ggccctctcc ggcacctgcc agagcgccga ctgcaccatc
    32581 gtgctcgacg cgcgactgcc gcgcaccctt gccggtatac tggcaggcgg tgcgcttggg
    32641 cttgccgggg cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt
    32701 ggcgtaaacg ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca
    32761 tccgcgcagg aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt
    32821 gcctttaccg gcagccaggg cggcgggcag ttaagtccgg tgcgtttaac cctggcgggc
    32881 gtggcactgg cggcggtgct ggaagggctg accagcggca tcgccctgct taatcctgac
    32941 gtctacgatc aactgcgttt ctggcaagcc ggttcgctgg atattcgtaa tttacacacc
    33001 ttaaaagtag tgctgatccc ggtgctgatc gccggagtaa ccgcgctatt actgagccgc
    33061 gcactgaaca gtttaagcct tggcagtgac accgcgacgg cgctgggcag tcgcgtggcg
    33121 cgcacacagt tgattggtct gctggcgatt accgtgcttt gcggtagtgc aacggcggta
    33181 gttggcccga ttgcctttat tggcctgatg atgccgcata tggcacgttg gttggtgggt
    33241 gccgatcatc gctggtcgct gcccgtcaca ctgcttgcta cccctgccct gctgctgttt
    33301 gccgatatca tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt agtcagtgcc
    33361 tttattggcg caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggcgcatga
    33421 tttacgtctc tcgccgatta atcatcacct gtttgctgct aattaccgcc tgtgtggttg
    33481 caggtatctg gggattacgc agcggtaccg tcacgctgga aacctcgcag atattcgccg
    33541 cgttgatggg cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac
    33601 gcgtgctgat ggcgctgtta attggcgcgg ctctgggcgt cagcggcgcg atttttcagt
    33661 cgctgatgcg taacccactc ggcagtcctg acgtaatggg ctttaacacc ggggcgtgga
    33721 gcggcgtgct ggtggcgatg gtgctgtttg gtcaggacct gacggctatc gcgctggcag
    33781 caatggcggg cggcattgtc acctcgctgc tggtctggct gctcgcatgg cgtaacggca
    33841 tcgacacctt taggttgatt attattggta tcggcgttcg cgccatgctg gtggccttta
    33901 atacctggct gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg
    33961 ccggatcgct caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat
    34021 tgatgctcat tgccgccgcc ttactggtac gccggatgcg cttgctggaa atgggcgatg
    34081 ataccgcgtg tgcgctgggc gtcagcgtcg aacgttcgcg gctgttaatg atgctggttg
    34141 cagtggtgct taccgctgcg gcaacagcgc ttgccgggcc gatttccttt attgctttag
    34201 tcgcgccgca cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg
    34261 cgctgtgcgg ggcgttgtta ttactggcgg ccgatctctg cgcccaacaa ctgtttatgc
    34321 cgtatcaact tccggttggc gtcgttaccg tcagcctcgg tggtatttac cttatcgtct
    34381 tgttaattca ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gtggcgaaca
    34441 gttaaccctc ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga
    34501 tggtcacttc acggcaatta tcgggccaaa tggctgcggt aaatccacgt tactgcgtac
    34561 cttaagccgc ctgatgacgc ctgctcacgg gcatgtctgg ctggatggtg agcacattca
    34621 acattacgcc agtaaagagg ttgcacgccg gattggcctg ttggcgcaaa acgccaccac
    34681 gccgggagat atcaccgtgc aggagctggt ggcgcgtggg cgttacccgc atcaaccgct
    34741 gtttacccgc tggcgcaaag aagatgaaga ggccgtcaca aaagcgatgc aggccacggg
    34801 aatcacccat ctggcggatc aaagcgtgga taccctctcc ggtgggcaac gccagcgagc
    34861 gtggatcgcg atggtgctgg cccaggaaac ggcaattatg ctgctcgatg aaccaacgac
    34921 ctggctggat atcagtcatc agattgatct cctggaactg ttaagcgaac tgaaccgcga
    34981 gaaaggctat accctggcgg cagtgctgca cgatcttaat caggcctgtc gttacgccag
    35041 ccatttgatt gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt
    35101 cactgctgaa ttgatcgagc gcatttatgg tctgcgctgc atgattattg acgatccggt
    35161 agccggaact ccgctcgtgg tgccgctcgg acgaacggca ccttcaaccg ccaagattta
    35221 aactaagtgc tcttccatca cggcatcctg ttttctggat gccatcgcat ggcgcaacag
    35281 cacgccacca caagccacca tcccgccgat catcgcagaa aggatcacaa taatcgcctt
    35341 acccggaccg tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa
    35401 attcacatcg ttgatatttg cttttgttaa ctgctcaaca agatactgac gattacgtaa
    35461 ttcaccgttc agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat
    35521 accgtctgcg ccgagagaaa tagagaaatc gaggtcatct ttaacggcct gaccattact
    35581 gtaaacgggc tttttaattc ctgccgcgtt agcaatgtcg agtgaataat tgaggcgctg
    35641 aatgtttgca tcaagttgat ttttcgtttt aatgcggtcc tgagccagtt tttctttttc
    35701 aaactgggtt ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa
    35761 cgcagagata taatcgatat accctctcaa aacggtctgc gcctcttcac tggttggcgc
    35821 ggtaaaactt agcgtccagg agttatacag tgacggttca tcttttttct tactggcatt
    35881 gtcatcaacc gctttcattt tttcgctcaa tgcgacaatt gcgcgatgca aatccagctc
    35941 gtcgattttc gcctctttta attggtccat cacataaggt gttgagcgca ggtactcttc
    36001 cagcaagcta accgactgaa acttcttgat aaacaggtta aatgcttctg tacgatcaat
    36061 tttgatatcc agatccagca cacgaagctt agtgaatgtt ttctcaagcg cctgccactg
    36121 aacaggttct ggaggcgtga caaccgccgc gctggtccat ttttgcggca ggatgaaaga
    36181 gatcagtaaa cctgcgcagg caaatgcaaa aatgaccgcc atgaccgttt ttttggcccg
    36241 ccataaaact gcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg
    36301 atactcggga aaatgagcgt cacttccctg tttaatattc agtgatgaca tggggtataa
    36361 cctgaaaatg agtccaatag ggggaaatct ccacggcgct cactttacca gttggtgaga
    36421 attatccgaa gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa
    36481 gagaaatacc caccaaactt ataaattaaa taaatgataa acccataatc atttcctgta
    36541 attatgggtt tatggataat attaatttac ctgtttagcg tagcgcgaat aatcggccca
    36601 attttttcaa acgcccctgg agagataata tccacatgcg cacaatcctg acgatagata
    36661 tccagctccg caatccacgg cgaccaggcg cgttcgggat tcataccttc ctgaagtgtg
    36721 cgttcagcaa caaacagcgt cgctttacct tcaaacggta cgctatgagc tgtcgtcagc
    36781 aggcgcacag catcagcgta gttgccttca atggtggtaa acaaatccgt tgaagtactt
    36841 ccctgttgtg ccgccaggaa ggcctcgcgc tcgcggttaa tctccgccag cacttccggg
    36901 tccagaccat tagcctcttt ttcctgccag ttttgcgttt ctggcggcca ggtatccagc
    36961 aagccaagaa atgccacctg ttcgccacgg gcacgcagtc gcgccgcaat accctgcgcc
    37021 agcgtaccgc ccagcgaata ccccagcagg taataagggc cgtgcggttg ttgttcaagt
    37081 aacgttgcca gatgcgcttc gcagacttca tccaggtttg ccgcggtctg catggggcca
    37141 tgagggcgcg gcgactgaat gccaataatc gaccattgtg gatcgagata acgcgaaagc
    37201 acgctaaact gccaggcaaa accggaggcc ggatggaaac aaaacagcgt cgggccatta
    37261 ccttcacgca acggcagaat ggtttcgaat cccatgcgcc gggagctgtc ttcttcacca
    37321 tcaataatcg ttgccagttt ggcgacagtt gacgcgacca tcacctgccc cggcgtcacc
    37381 tggcgggtaa actgccgact taactgcgca gccagtttca ttgccagtag cgaatgaccg
    37441 ccaagcgcaa agaaatcagc atcggcatcc tgcacgtcac aacccagcaa cgacgagaat
    37501 gccgcggcga taatcgtttc actgcccgct ttcggcgcac gccccggcgc ttgtgccttc
    37561 agttcaggca acggtaaggc tttgcgatcc agcttgccgt tggcgctaag cggcaactgc
    37621 ggaagttgca gcagaaccac gggcaccata tgcggtggca atgtttcgcg caactgcgcc
    37681 tgcaatgcag tggtatccag cggcaggcct gattgcgaca ccagataccc aaccaactga
    37741 cgcgcatcgc ctcccgttgc ggcagcctgg ttaatgacgc aggcgtgggt aacggcctgt
    37801 tcgacatccg gcagtgcctg catcacgcga tcgatttcgc ccagttcgat acgctgcccg
    37861 cgaattttta gctgatcgtc actgcgcccg aggtactcca ccgcgccgtt atccagccag
    37921 cgggcaacgt ctccggtacg gtacatccgt tcgccagggg caaaaggatc ggcaataaag
    37981 cggctggcgg tcagatcggg tcgtccaaga tacccctgcg ccagttgaat accggtgaga
    38041 tagagatctc ccgccacacc cggcggcacc ggatgcatta ttgcatcaag aatacgtagg
    38101 cccgtattcc acaccggata accaatcggc acactgctgc cgcgcacctg tgccagttcc
    38161 tcgccaaaag ccggatacca gctgacatct accgccgctt ccgtcgggcc atataaatta
    38221 tgcaacgccg cgctggttaa ctgttgccat tcgcggcata aatcagccgg taaggcctca
    38281 ccactacaga aaacctgttt taacgtcgcg caattctggc gagcggtttg cggagtcagc
    38341 gatgcaacaa atgccgccag catcgacggc acaaagtgcg tggtcgttac gccatattcg
    38401 gcaaagaatt gctgcatagc gagcgggtcg cggtgcgctt ccggttcagc catcaccagt
    38461 ttagccccgg caataaacgg ccagaaaaac tcccacaccg agacatcaaa actgcacggc
    38521 gttttttggg caacgacatc ttcgcctgta agcgggtagt ggttttgcat ccaaagcagg
    38581 cggttaacga tagccgtctg cccgaccatt acccctttcg gcctgccggt ggagccagag
    38641 gtaaagatga tataagccgt gtggtgcggt tgtgaaagtt gcagcggcgc actgccctgc
    38701 ggtgtaagcg gggcgttata gcaaaggctg gttaaatctg gaacatcggc aaagcgcggc
    38761 agttgatcgt cggtggtgat taacagcgac ggacgcgcat cttccagcat cattttcagg
    38821 cgatcgtccg gatagccggt atccagcggt aaccaggccg cacctgtttc aacaatcgca
    38881 tgtagcgcca gagtcaaaaa taccgagcgc ggtagcgcca ccgccacgct gtcgcctggt
    38941 ttaacgccgc gctcacgcag cagattcgcc agcgccacca cctgctcgcg catttccaga
    39001 tagctgaact ggtaacgcgc atctgccagc gccggagcat ccggtgtttt tgccgcctgt
    39061 tccgccacca gcgcgctcag cgtggtttct ggaatctcaa cctgagtggc gttgatctgc
    39121 gccagctgca catattcacc tggcagcata atatcgacat cgccgcacaa cagcgccgga
    39181 tccgcggcga actgggcaat cagcattttc aggcgttcag cgtgttgaat taacgttggc
    39241 tcatcgtaac gctgtttatt ggcgaggatc tcaatactca aatcaccgtg ttcatccggg
    39301 aacagtgcca gttcaaggtc attaaccgga ccggttgcca gggtatgggt ttgcgcctga
    39361 acaccaggaa tatccagttg gtaatcaaat accttgatat tgagtaccgg accaaacagc
    39421 ggttcatcac ctgccgctcg cccgctgtca cggacaattt gttcggcatc gtaacgttga
    39481 tgacgacgca tttttttcag ttgtgctgcc agtcgggttg ccagttgcgg cagcgtttct
    39541 tgcgcctcaa tatgaatacc caacggcaaa acgttgagca cgggtccggt agccgtcagc
    39601 gccgccgagc ccagtcgacg cataaagata aatccggcgg cgtagtccat gcgattgcac
    39661 aatcgcccca gccataaggc caccagcgca agggctaaat cggtacgctg cacacctgaa
    39721 agttgcgtag ccagctggcg gaattccccg tcagtaaatt ccagtttcag gcgaagaata
    39781 tctgccgaag cgctgcgccc cggtaaaggt gccggagaaa gtgacgcggg cggcggcaat
    39841 tgacgacgct gttccgccca gaatgccgca tcacgctgcc aggcttcgct ttcgcggtat
    39901 tgctggtact cttccactac atcagcgaaa ggcgtaaacg gcgaagcagg cgttggttcg
    39961 ccacgcagcc atgcgcagta aatattggcg atctggcggg taatggccgg gaaactgaag
    40021 ccatcgacca gcaaatggtg ataacgctga taccagtacc agcggttatc cgccacctgt
    40081 atcaactgat gaaagaccag tggtttaccg ctatcgacgc gcaaatcttg ttgcaaatcc
    40141 gcctgcatta atgcccgcgc agtaccgtgc ggatcaatgt tggttcgcag gtcgataatt
    40201 tctggcaatt cgaacgtctg cgcatcatcg acccactgcc agacttcgcc gttatcttcc
    40261 gtaaaacgca tccgcagcgt atctgcttgc gctaatccgg caaccaccgc gcgggccagt
    40321 aatggcgcat caacctctcc ggttaactca acgtaatgcg ccacgctcca ggcggagggt
    40381 aaatccgaca gtttttctgc catccagatg ccgggctgtg cggcgaccaa aggtaaatgc
    40441 tggctcattg tgcctcctgc aactgggtga aattcgtcgg tgtcagagta cgccagtggg
    40501 cttccagcca ctgctggcag gacgcctgtg actgcggctg acacacaacg tcccagcctg
    40561 ccggtaaggc gcattgttgc ggccacagac tgaattgccc ctgcgcattg cgcaaaatgt
    40621 aaaacgctcc ctgcggatca tcgaagggat tactgaatgc catattcaac tcctgtcatg
    40681 gaaaagtggt tgccagaggt cgattagccc ctgcatcaag ccaccgcgcc aacaaagcgc
    40741 atcatgtccg ccgtcaacct gacgccagaa aatggattct tttatggggt gtaattgcgc
    40801 atacagcgcc tgattggccc gcatgatcat cggctcgcga atacccgctt ccagcacaat
    40861 gcgcagacct tcggcgctaa cttcaccagc tttaagttgc tcaagtaaaa caccttcttg
    40921 ctgcccgccc cgatgcggcc accagtacga tcctgactgg cttaatacac agccaaagcg
    40981 ttcaggccag tgcagtccgg catacagcgc ggaaagccca ccaaaactct gcccggcgac
    41041 tacggtgcga tcggcgcgat cgctaaaagg ggcaatagct ttcaccaggg gtaataactc
    41101 ttgctgtact gccagccaga aatccgcatt acacggcagt tcgtgggcgc ggtgcgtggt
    41161 gtcaatagcg tcgatcaaca catacacggc gggaggaagt tgctgacgat gggtcaacga
    41221 agtcagcaca ggccagacag gcatactctg cgcccaaaat tcgccatcga gcaaaactgc
    41281 cagcgggcgt tctcctgctg ttgcatcgcc ggtggtaaaa atccatacac ggcgtgaatt
    41341 tttcaaccgc tcacttttcc agataatttc tttggcaggc gtttctggcg cttgcggaca
    41401 atcccatccc ggttgtaggg gcgcttgcgg catttcgagt gcagaaacag cgtgccctcg
    41461 cccgcctttc cagctttgtg gattcagcgg atcggctatc gcctggggca atagttttcg
    41521 ccagccttca cgtaattcga ggcgatcggg ggatggtgca gaaaaaatgt catcgcgttc
    41581 ggtgggaata aaacagtagc tgccgcgcca gttggcatta agttgtgtcg tccactgcca
    41641 gacgttggta cctgcaattc gctgcatcga ctggggctgg ctgttctgat ggtgatcggt
    41701 cacaccagtg atgtagaccc atacgcgctt tatcgtcgag tattcttcag aaccctgggg
    41761 atcacgccac cagaaagtga tctcaaacat ttcgtcattc agacgctgcc attccgggcc
    41821 atgtttcgac tgccaccagc tctcacttcc tacttttaac gccgtcacac cataacccca
    41881 tgtttactgt gcaatttttt attgattgca gaaatatatt gataatatta ttgataacta
    41941 tttgcatttg caatagcgta atggcgcgcc gtgggaagcg cggacattaa ttaaccaact
    42001 gcactgcgtg tctttcagga tcaaaggttt tcgcggtagc gggatgcgtc gtgttgatga
    42061 cgaccatgcc cgacagttgc aattcgtggc aaaaatgcag gaataaaaca atgaacaaga
    42121 agattcattc cctggccttg ttggtcaatc tggggattta tggggtagcg caggcacaag
    42181 agccgaccga tactcctgtt tcacatgacg ataccattgt cgttaccgcc gccgagcaga
    42241 acttgcaggc acctggcgtt tcgaccatca ccgcagatga aatccgcaaa aacccggttg
    42301 cccgcgatgt gtcggagatc atccgtacca tgccaggcgt taacctgacc ggcaactcca
    42361 ccagtggtca gcgtggtaat aaccgccaga ttgatattcg cggcatgggt ccggaaaaca
    42421 cgctgatttt gattgacggc aaaccggtaa gcagccgtaa ctcggtgcgt cagggctggc
    42481 gtggcgagcg cgatacccgt ggtgatacct cctgggtgcc acctgaaatg attgaacgta
    42541 ttgaagttct gcgtggtccg gcagctgcgc gttatggcaa cggcgcggcg ggcggcgtgg
    42601 ttaacatcat taccaaaaaa ggcagtggtg agtggcacgg ctcgtgggac gcatatttca
    42661 atgcgccaga acataaagag gaaggtgcca ccaaacgcac taactttagc ctcaccggtc
    42721 cgctgggcga cgaattcagc ttccgcttgt atggcaacct cgacaaaact caggctgacg
    42781 cgtgggatat caaccagggc catcagtccg cgcgtgccgg aacgtatgcc acgacgttac
    42841 cagccgggcg cgaaggggtg atcaataaag atattaacgg cgtggtgcgc tgggacttcg
    42901 caccattaca atcgctggaa ctggaagcag gttacagccg ccagggtaac ctgtatgcgg
    42961 gcgataccca gaacaccaac tctgactcct atacccgctc gaaatatggc gatgaaacca
    43021 accgtctgta tcgccagaac tactcgctga cctggaacgg cggctgggat aacggcgtga
    43081 ccaccagcaa ctgggtgcag tacgaacaca cccgtaactc gcgtattccg gaaggtctgg
    43141 cgggcggtac cgaagggaaa tttaacgaaa aagcgacaca ggatttcgtc gatatcgatc
    43201 tcgatgacgt gatgctgcac agcgaagtta acctgccgat tgatttcctc gttaaccaga
    43261 cgctgacgct gggtacggag tggaatcagc agcggatgaa ggacttgagt tccaacaccc
    43321 aggcgctcac cgggacgaat accggcggcg ctattgatgg cgtgagtgcc accgaccgta
    43381 gcccgtattc aaaagcagaa attttctcgc tgtttgccga aaacaatatg gagctgactg
    43441 acagcaccat cgtaacgccg gggctgcgtt tcgatcatca cagcattgtt ggcaataact
    43501 ggagtccggc tctaaacata tcgcaaggtt taggcgatga cttcacgctg aaaatgggca
    43561 ttgcccgtgc ttataaagcg ccgagtctgt accagactaa cccaaactac attctctaca
    43621 gtaaaggtca gggctgctat gccagcgcgg gcggctgcta tctgcaaggt aatgatgacc
    43681 tgaaagcgga aaccagcatc aacaaagaga ttggtctgga gttcaaacgc gacggctggc
    43741 tggcgggtgt gacctggttc cgtaacgatt atcgcaataa gattgaagcg ggctatgtgg
    43801 ctgtagggca aaacgcagtc ggcaccgatc tctatcagtg ggataacgta ccgaaagcgg
    43861 tggttgaagg tctggaagga tcgttaaacg taccggttag cgaaacggtg atgtggacca
    43921 ataacatcac ttatatgctg aagagtgaaa acaaaaccac gggcgaccgt ttgtcgatca
    43981 tcccggagta tacgttgaac tcaacgctga gctggcaggc acgggaagat ttgtcgatgc
    44041 aaacaacctt cacctggtac ggcaagcagc agccgaagaa gtacaactat aaaggtcagc
    44101 cagcggttgg accggaaacc aaagaaatca gtccgtacag cattgtgggt ctgagcgcga
    44161 cctgggatgt gacgaagaat gtcagtctga ccggcggcgt ggacaacctg ttcgacaaac
    44221 gtttgtggcg tgcaggtaat gcccagacca cgggcgatct ggcaggagcc aactatatcg
    44281 ccggtgccgg ggcgtatacc tataacgagc cgggacgtac gtggtatatg agcattaata
    44341 ctcacttctg atgctaacgt cagattgttg acaaagtgcg cgtagttcat gccggatgcg
    44401 gcgtgaacgc cttatccggc ctacaaaaat cttgccaatt caatatattg caggaccacg
    44461 taggcctgat aagcgtagcg catcaggcag ttttgcgttt gtcatcagtc tctaatatgg
    44521 tcgacatgaa aactacgcat accgccctcc cctttgccgg aaatacgctg cattttgtta
    44581 agttcgatcc ggcgagtttt tgtgagcagg atttactctg gctgccacac tacgcgcaac
    44641 tgcaacacgc tggacgtaaa cgtaaaacag agcatttagc cggacggatc gctgcagttt
    44701 atgcgctgcg ggaatatggc tataaatgtg tgcccgcaat cggcgagcta cgccaacctg
    44761 tgtggcctgc gggggtatac ggcagtatta gccactgtgg ggctacggca ttagccgtgg
    44821 tatctcgtca accgattggc attgatattg aagaaatttt ttctgcgcaa acagcggcag
    44881 aattgacaga caacattatt acaccagcag aacacgagcg actcgcagaa tgcggtttaa
    44941 ccttttctct ggcgctgaca ctggcatttt ccgccaaaga gagcgcattt aaggcaagca
    45001 agatacaggc cgctcaaggt tttctggatt atcagataat tagctggaat aaacagcaga
    45061 tcatcattcg actagaggat gagcagtttg ctgtgcactg gcagataaaa gaaaaaatag
    45121 tcataacgct gtgccaacac gattaattga caacatctgg tacgattcgc cgcagccatt
    45181 actgaccgcg ggcgaaagtg taaagcaggt gccttaccat cctgacctga caaccggata
    45241 tgcggggatt gctccccgca caacggctac ttcttcggtt cgtaagcgag aacagcctta
    45301 aactcaatat tacgttcctt caccgtaaac tcacacagcg agtcgcggac cagaagcgta
    45361 aatcccagca ccgttaaaca cagcactatg actgccacaa gggcatattt cgtcagcatg
    45421 tttatattgc ctccatgcga agaagggact accatccgag ttgttcaggt ttgggtaggt
    45481 agccccaggg ttgatagaaa tatcgtcctg gggcttcatc tttccagacc tcagagttaa
    45541 cctgaaaccg gaaagcttca ggcacccgcc gttatcttac ccttcctcag caaaatagct
    45601 gtttatttat acagtgaatc ctgtttcgga cttctgttgg caaaactgat cattgccctt
    45661 aaaacgacat cgcccgcagc cattgctgac cgcgggcgaa agtgtaaagc aggtgcctta
    45721 ccaccctgac ctgacaaccg gatatgcggg gattactccc cgcacaacgg ctacttcttc
    45781 ggttcgtaag cgagaacagc cttaaactca atattacgtt ccttcaccgt aaactcacac
    45841 agcgagtctc cgaccagaag cgtaaatccc agtaccgtca aacacagcac tatgactgcc
    45901 acaagggcat atttcgtcag catgtttata ttgcctccat gcgaagaagg gactaccatc
    45961 caaattgtct ggtttggagt ggcagcccca gggttgatag aaatatctcc tggggcttca
    46021 tctttccaga cctcagagta aacctgaaac cggaaagctt caggcacccg ccgttatctt
    46081 acccttcctc agcaaaatag ctgtttattt atacagtgag tcctgtttcg gacttctcac
    46141 tcctaatttg atcaactgaa gctgaacccg tttttttctg catgcctcaa tcgtcaaaat
    46201 tcttcgccac cagccagctc tgccataact tgtcacattg caaaagtagc caaattgctt
    46261 cagtcaccat caccaaaaaa aagaaacatt ccagaacttc cgcatctcgc ccagccgctc
    46321 cgacttctat actgaataga aaacgccaac ataagagaaa cctatgccat tacccgattt
    46381 tcatgtttct gaacctttta ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc
    46441 gggctatgac ttaagccagg actcttcaat gctgattgac gcggttaaaa ataagatcac
    46501 ggccggagag gtaaagcacg atatcaccga aagtatgctg gagctggcga cggatgtttg
    46561 ccgtgatatc aaccaggctg ccgggcaatt ttcagcgatg cagaaagtcg tattgcaggc
    46621 agccgcagat catcatctgg aaatttgcgg cggtggcacg cacccgtttc agaaatggca
    46681 gcgtcaggag gtgtgcgata acgaacgcta tcaacgaacg ctggaaaact ttggttatct
    46741 cattcaacag gcgaccgttt ttggtcagca tgtccatgtt ggttgcgcca gtggcgatga
    46801 tgccatttat ttgctgcacg gcctgtcacg gtttgtgccg cactttatcg ccctttccgc
    46861 cgcgtcgcca tatatgcagg gaacggatac gcgttttgcc tcctcacgac cgaatatttt
    46921 ttccgccttt cctgataatg gcccgatgcc gtgggtcagt aactggcaac aatttgaagc
    46981 cctgtttcgc tgcctgagtt acaccacgat gatcgacagc attaaagatc tgcactggga
    47041 tattcgcccc agtcctcatt ttggcacggt ggaggttcgg gtaatggata ccccgttaac
    47101 ccttagccac gcggtaaata tggcgggatt aattcaggct actgcccact ggttactgac
    47161 ggaacgcccg tttaaacatc aggagaaaga ttacctgctg tataaattca accgtttcca
    47221 ggcctgtcgc tatgggctgg aaggcgtcat taccgatccg catactggcg atcgtcggtc
    47281 actaatggaa gacaccttgc gtttactgga aaaaatcgcc ccttctgcac ataaaattgg
    47341 cgcatcgagc gcaatagaag cactgcatcg ccaggtcgtc agcggtctga atgaagcgca
    47401 gctaatgcgc gatttcgtcg ccgatagcgg ctcgctgatt gggctggtga aaaagcattg
    47461 tgagatctgg gccggtgact aagccgggaa ttgccttaca gaagaatatc cctgacaatg
    47521 gtgttttaac ttacatttaa caatgttatg aaacaccctt tagaaacctt gaccaccgca
    47581 gcaggcattt tgctgatggc tttcctttct tgcctgctgc tgcccgcccc cgcactgggg
    47641 ctgacgctgg cacaaaaact ggtgaccatg ttccatctga tggatcttag ccagctttac
    47701 actttattgt tttgtctgtg gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc
    47761 tttatctggc gacgctggtt ctcgctggcg gattaaacgt cgattatcgc cgtacttcgc
    47821 ggcatacttt gcttattctc cttcgcctaa aggaacgttt atggataagc aatcactgca
    47881 cgaaacggcg aaacgcctgg cccttgagtt accctttgtc gagctttgct ggccttttgg
    47941 cccggagttc gatgttttta aaattggcgg caagattttt atgttgtcgt cagagctgcg
    48001 cggcgttccc tttatcaatt tgaagtccga tccacaaaaa tccctgttaa atcagcagat
    48061 atacccgagc attaagccag gatatcacat gaataaaaag cactggattt cagtgtatcc
    48121 cggcgaggaa atctccgaag cgttacttcg cgatctgatt aacgattcgt ggaatctggt
    48181 ggttgatggt ctggctaaac gcgatcaaaa aagagtgcgt ccaggctaaa gcggaaatct
    48241 atagcgcatt tttctcgctt accatttctc gttgaacctt gtaatctgct ggcacgcaaa
    48301 attacttaca catggagtct ttatggatat catttctgtc gccttaaagc gtcattccac
    48361 taaggcattt gatgccagca aaaaacttac cccggaacag gccgagcaga tcaaaactct
    48421 actgcaatac agcccatcca gcaccaactc ccagccatgg cattttattg ttgccagcac
    48481 ggaagaaggt aaagcgcgtg ttgccaaatc cgctgccgat aattacgtgt tcaacgagcg
    48541 taaaatgctt gatgcctcgc acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc
    48601 ctggctgaag ctggttgttg accaggaaga tgccgatggc cgctttgcca cgccggaagc
    48661 gaaagccgcg aacgataaag gtcgcaagtt cttcgccgat atgcaccgta aagatctgca
    48721 tgatgatgcg cagtggatgg caaaacaggt ttatctcaac gtcggtaact tcctgctcgg
    48781 cgtggcggcg ctgggtctgg acgcggtacc catcgaaggt tttgacgccg ccatcctcga
    48841 tgcagaattt ggtctgaaag agaaaggcta caccagtctg gtggttgttc cggtgggtca
    48901 tcacagcgtt gaagatttta acgctacgct gccgaaatct cgtctgccgc aaaacatcac
    48961 cttaaccgaa gtgtaattct ctcttgccgg gcatctgccc ggctatttcc cctcagattc
    49021 tcctgatttg cataaccctg tttcagccgt catcatagtc tgctgttgta taaaggagac
    49081 gttatgcagg atttaatatc ccaggttgaa gatttagcgg gtattgagat cgatcacacc
    49141 acctcaatgg tgatgatttt cggtattatt tttctgaccg ccgtcgtggt gcatattttt
    49201 ttgcattggg tggtactgcg gaccttcgaa aaacgtgcca tcgccagttc acggctatgg
    49261 ttgcaaatca ttacccagaa taaactcttc caccgtttag cttttaccct gcaggggatt
    49321 atcgtcaata ttcaggcggt attctggctg caaaaaggca ccgaagcggc agatattctg
    49381 actacctgcg cgcagttgtg gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg
    49441 gatgttattt tgaatctggc gcagaaattc ccggcggcat ctcagttacc gctgaaaggg
    49501 atttttcagg ggattaaact gatcggcgcg attctggtcg gcattttgat gatctcgctg
    49561 ctgattggtc agtcgccagc gattctgatc agcggtcttg gtgcaatggc tgccgtgctg
    49621 atgttggtat ttaaagatcc gattcttggt ctggtggcag gtattcagct ttccgcgaac
    49681 gatatgctga aactgggcga ctggctggag atgccgaaat acggcgcgga tggcgcggtg
    49741 atcgatattg ggttaaccac cgtcaaagtg cgtaactggg acaataccat taccactatt
    49801 cccacctggt ctctggtctc tgactccttt aaaaactgga gcgggatgtc agcatctggc
    49861 gggcgacgta ttaagcgcag tatcagtatt gatgtcacca gtattcgttt tcttgatgaa
    49921 gacgaaatgc aacgtctgaa taaagcgcat ttgttaaagc cttatttaac cagccgccat
    49981 caggaaatta atgagtggaa tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc
    50041 cgaatgacca atattggaac ctttcgtgcc tatctgaacg aatatctgcg taaccatccg
    50101 cggattcgta aagatatgac tttaatggta cgccaactgg ctccaggtga taacggttta
    50161 ccgctcgaga tctatgcgtt taccaacacc gtggtgtggc tggaatatga aagcattcag
    50221 gctgatatat tcgatcacat atttgcgatt gtcgaagagt ttggtctgcg acttcatcag
    50281 tcgccaaccg gcaatgatat tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa
    50341 ccgctctcat cgaatggatg agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt
    50401 atttccgacg cagcgtttta aatgccacaa acaggaatac aatccacacc ggcagcagga
    50461 tcgctgacaa gcgcatatca tccatcgtgc acatcagcag caaaatcatg ccgaggaagg
    50521 caatacaaag atagttgccg aacggataaa gcagcgcctt aaactgtgtt tcacgtccct
    50581 gacgtcgcat cgccgcgcga aaacgcagat gcgccaggca gatcataatc cagttcaaca
    50641 gcagcgttgc taccaccagc gccatcagca gaccaaacgc tttttgcggc agcagatagt
    50701 tgattaacac caccagcgaa gtgatcgctc cggaaagcat cagcgagtta atcggcacgc
    50761 cgcgacggct gacgcgagtc aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca
    50821 gcatgcggct gttagagtaa accccgctgt tatacactga cagcgatgct accagaatga
    50881 cgaagttcag cgcagaagct accacgttgc tgtcgagatt atggaaaatc atcacaaacg
    50941 ggctactgtt ggatttcact tccacccacg gatagagcgc cagtaaaact accagtgaac
    51001 cgatataaaa cagcaggatg cgatacacta cctgatttac cgctttcgga atgctttttt
    51061 ccggatcgcg cgcttcagcg gcagtaatcc caatcagctc cagaccgccg aaggagaaca
    51121 taatcaccgc cagcgacaaa atcagcccat tccagccggt ggcaaagaaa ccaccgtagc
    51181 gccagaggtt gtcgatactg gctttctcgc caccgtgacc agaaaacagc agccacaggc
    51241 caaagccgat cataccgatg atcgccagca ctttaatcag cgcaaaccag aattcggttt
    51301 cgccatataa gcgcacgttt accaggttaa cggcgttgat gataataaag aaggcggcag
    51361 cccaaatcca cgttggaaca tccgggaacc agtactgcat atagatgccc gcagcggtca
    51421 gctctgccat tcccaccagc acgaacatta cccagtagtt ccagccagag aggaagcccg
    51481 caaacggtcc ccagtattta taggcaaagt gggcaaatga accggatacc ggctcttcga
    51541 ccaccatctc gccgagctgg cgcataatca ggaaagcgat gatcccggcg acgccgtagc
    51601 ccagcaatac agccggaccc gccatctgaa tcgccgggcc aatgccaaga aacagaccag
    51661 taccaattgc gccacccagc gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa
    51721 gcgtcggctc ttgattcgac gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt
    51781 tcccctgtgt gtcttttttg ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa
    51841 gacacaatcc acacggttaa accgggtatc ctgcttttat ttccgtaccc gatgtcggtg
    51901 cagccacatc agcttatacg ccgccgggat aataaacagc gatagcaaag gcgcggtgat
    51961 catgccgcca atcatcggcg cggcaatccg gctcatcacc tctgaacctg ctcccgttcc
    52021 ccacagaatc ggcagcagac cggcgataat cactgccacc gtcatcgctt tcgggcgcac
    52081 gcgcaggacc gcgccgtgat acaacgcctc atccagcttc tgctcgctga atgtttgcgg
    52141 attattcaac gacggctcga cctctatggc gtgacgtaaa tacatcagca tcaccacgcc
    52201 aaattcggcg gcgactccgg cgagggcaat aaaaccggtg cccgtcgcca cggaaagatg
    52261 aaagcccatc caccacagca accagatgcc gcccaccagc gcaaacggta cgctgctgat
    52321 aatcagcaac gcttcgccca cccgacggaa cgccagatac aacagcacga agatgatcat
    52381 cagcgtcatc ggcaccatga gtttcagctt atggttggcg cgctccagta gctcgaactg
    52441 cccggagaat gtcacgctgg tgcccggttt taactgcact ttttcagcta tcgctttttg
    52501 caaatcgtga accaccgaca ccatgtcacg atcgcgggca tcgatataaa tccagctcgt
    52561 cgggcgcgca ttctcggttt tcagcatcga cggtccggta gagactttaa cgtcggccac
    52621 gtctgccagg gtgatttgct gcttcatcgg cgtcaggatc ggcaactggc gcagtgcctg
    52681 cggactatcg cgccagcttt gcggataacg cagattaatt ggataacggg caatcccttc
    52741 caccgtttcg ccaaccatcg ccccgcccac cgcagaagtc acaaacaact gcacatccgc
    52801 caccgtcata ccgtaacgtg cggctttttc acggttaatc tcaacgttga tatagcgccc
    52861 accttccaga cgctcggcaa gcgcagaagc cacgcctggc accgttcgtg ccacctcttc
    52921 aatttgctcc gccatcgcgt cgatatccgc cagcacagtg ccggaaactt taatgccgat
    52981 ggggctctta atgccggttg agagcatatc gatacggtta cgaattggcg gcacccacag
    53041 attcgccagc cccggcagac gcacggtgtt atccagttcc tcaatgattt tgtccatcgt
    53101 catgcctggc cgccactgat cttgtggctt aagctggatg gtcgtttcta ccatctccag
    53161 cggagcagaa tcggtggcgg tttccgcttt cccggttttg ccaaataccc gcgccacttc
    53221 aggtacgctc ataatcagct tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga
    53281 aatccccggc agcgtcgatg gcatatacaa caagtcgcct tcattgatct gcggtaaaaa
    53341 ttccccgcca actttattga gcggccagag aaccgtcagc accgaaagcg ctgccaccag
    53401 cagcgtggtt tttggccagt gcagtacttt cagcaacagc ggatgataag cacgaatcaa
    53461 aaagcgattg agcgggttac tgctttctgg cggaattttg ccacggatcc agtaacccat
    53521 caggataggg atcactacga tcgccagcag cgccgcaccc gccatcgcat acgttttggt
    53581 gaacgccagc gggccaaaca gacgcccttc ctgcccttcc agggtgaaga tcgggataaa
    53641 cgacaacgtg ataatcagca gactgataaa cagcgccggc cccacttcaa cagatgcatc
    53701 ggtgatcacc tgccagcgcg ttttattatc cagcgtggcg tcaggatgct ggtgctgcca
    53761 ctcttccagc cgtttatgcg cattctcgat catgacgata gcggcatcga ccatcgcccc
    53821 gacggcaata gcaataccgc ccagcgacat aatattggcg ttcagtccct gaaagtgcat
    53881 gacaataaaa gcaatacaca accccagcgg caacgaaata atcgccacca gcgccgagcg
    53941 cacatgccag agaaacagcg cacagactac tgccaccaca ataaactctt ccagcaactt
    54001 gccgctgagg ttgtcgatgg cgcggtcaat gagctggctg cgatcgtatg ttgtaactat
    54061 ctccacgcct tccggcagac tacttttcag cgtttccagt ttgtctttca cggcggcgat
    54121 cacttctcgg gcgtttttgc cggagcgcag gatcaccacc ccgcccgcca cttcgccttc
    54181 gccgttcagt tcggcaatgc cccggcgcat ctccgggcca atctggacct tcgcaacatc
    54241 gcgcagataa acgggcacgc cattttcact ggcttttaaa acgatgtgat taaagtcgtc
    54301 gagcgtttgc agatagccgc tggcgcgcac catatattcc gcttccgcca gttcgatcga
    54361 cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt tttacttcgg cgagactgat
    54421 gccatattgc gccaggcgct gaggatcgat aaccacctga tactctttca ccacaccgcc
    54481 taccgacgcc acttccgcaa cgtcaggaat ggttttcagc tcatatttga gaaaccagtc
    54541 ctgcaatgag cgtaaatcgg caagatcgtg cttaccgctg cgatccacca gtgcatattc
    54601 atagatccag ccaacacccg tggcatctgg ccccagctcg gcgctgactc ccgcaggcag
    54661 cttaccctgt acctggttga ggtactccag cacccgcgag cgcgcccagt atggatcggt
    54721 gccatcttcg aaaatgacat acacataaga gtcgccaaac tgcgagaaac cgcgcacagt
    54781 cttcgcgcca ggcaccgaca acatggtggt agttagcgga taagtcacct gattttcaac
    54841 gatttgcggt gcctgaccgg gatagctggt tttaataatc acctgtacat cggagagatc
    54901 cggcagcgca tccactggcg tgttaatgat ggtccaggtg ccccagatac tcagaaacaa
    54961 cgcgcccatc agcaccagaa aacggttcgc caccgagcga cgaataatcc attcaatcat
    55021 tggttattcc ctcaatgcgc atgggtagca ctttcagagc gcatccgctc cagtgcgcca
    55081 gaaatattgg cttcggaatc aatcaggaac aggccgctgg aaaccacctt ttcaccttcc
    55141 gccagaccag agcgtaatgc ggtgacgcct tgcgatgcct ggaaaacggc aacgcgtttc
    55201 ggtacaaagc gcccgtcggc atcaacggta atcacccgct gttcgctgcc ggtatcaatc
    55261 agcgcttgtg acggaatgag cagcatcggt tcgctggcgg tgttgagttg caaccaggcg
    55321 ttcattcccg gctttagcgc ctcgtcggtg ttgtcgactt ccaggcgcag ctgcagcgtg
    55381 cgggttgcgg catccacgcc gggtagcagc gtccatttgc ggatggtgag tgttttatca
    55441 ggtcgtgccg gaacggtgag cgtaaactgc gaggcatctt tcaccagcca ggcgatggac
    55501 tccgggatcg cggcagtgat ccacaccgga tccataccct gaattttcgc gaccacgtta
    55561 tctttggcga tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc
    55621 gctttgagcg taaagcgagt ctggattttt tgcgtggcga tcaggcggcg aatatccgcc
    55681 tccggcattc ccgccagtct cagccgctca agaatgcctt cagtctgggt cgccgtaccg
    55741 ccagtttcgc gcagcagtaa atactcactc tgcgcttcca cccagtcggg aatggtcagg
    55801 tcgagaagcg gcgcgccctt ttgtacttta tcgcccacgg taagcggata caccttgtcg
    55861 ataaacccgg cggcgcgggc ctgcacaatg gcatactgat actcgttgta actgacattc
    55921 gccgggaaac tttgggcaaa agtcagcggc ccgcgcgtga cggtcgccgt tttcaccccc
    55981 agattctgcg tctgagtcgg gtcaatgcgc acaccagacg cagaactctc ttcatcggca
    56041 tatttcggca ccagatccat atccataaac ggcgatttac ctggtttatc gaaccgcgta
    56101 ttgggataca tcgggtcgta ccagaataag attttacgtt ctgcggtcgg cgttttttct
    56161 gcgggcggtt cagcctttgc aaaccaggta aaacctgccg cagaaataat accgcccgcg
    56221 atcatgctgc cgataataag cgcgattttt ttcatctcat taaacctggg ttactggctg
    56281 actttaatat cctgtaataa agaaaggttg ccctgctgga caaaattaaa cgccactttg
    56341 tcgccggttt taatttcact cattttcgtc tgcggggtga tggtaaagcg catggtcatc
    56401 tccggccagt tcacggctac aatcggatcg tgatggatgg tgattttttt gctttccaga
    56461 tcaacacctt ttaccacccc agtggcgcta atgacctgtg gttttgcttc gctcgtggtt
    56521 tcatgatgat gttcgttagc ctgggcatta aagccaataa cggtaaacag actgaacatt
    56581 gcgacttgca gtgctttttt catttattct ctcctggagt taaaaattat tgctgccaac
    56641 cgccccctaa ggcggtatac aaagaaattt cgttaacctg acgggcataa ttcaggtcga
    56701 gtaaagtttg tcgggttgca aataaagaac gctcggcatc cagcacttcc agataactta
    56761 ccgcgccgtg ctgatataat gcccgcgccc gttgcaaagt aatttgcagc gacgccagat
    56821 aacgctgctg ggcgctgatt tgatcgttca ggctttgacg taatgccagc gcatctgcca
    56881 cttctttaaa ggcgttctgg attttctgtt cataattcac taccgactgc tgctggcgaa
    56941 tttcggcgat atccagattg gcctggttgc gtccggcatt aaaaatgggg atctcaattt
    57001 taggaataaa attccacatc ccgctgctag cgttaaataa tgaagacaga tcgctgctgg
    57061 cggttgatat tccgctggtc aggctgatag acgggaaaaa tgccgcacgc gcagcgccaa
    57121 tattggcatt agccgccatt aacgcgtgtt cagcttccat aatatccggg cgctgcaata
    57181 agatttgcga cgacaatccc gccggtaatt taacgctttg caggctgtcg ctgtttactg
    57241 tctgcgcttg cggcagcttg ccgtagcttc ccaataacag ttgcaatgca ttattcgcct
    57301 gcgccagttc cccctgacgt ttagcgatgt cgctgcgggt actttcaatc accccgcgag
    57361 cctgttccag cgccagaaca ttactgctac ctgtcaacag ttgtttttcg acaaacgcat
    57421 atgactgctg ataattacgc agcgtttctt cggctatttg caattgcgca tacgctagct
    57481 gctgattgaa ataactttgc gcgacattag aaaccagcaa aatatgcacc gcgcgctggg
    57541 cttcctcagt ggctaaataa ttttggcgct cggcttcgct catgttcttt aagcgaccga
    57601 aaaaatcgag atcaaagctg gcgttaaggc cggtcgaaaa ctcccgtgtc gtggcggtat
    57661 tccctttaag attaccgctc cagctgccgc tgccctcgcc attgagctgt gggtagcggt
    57721 cggcatcggt cagacgatat tgcgcccgcg cttcctgcac tttcagcgtc gccatgcgca
    57781 aatcccggtt attcaccagc gcctcgctaa tcagcgtctt cacctgatta tcaataaaaa
    57841 aggtgcgcca gcccgcgttc tggtagttat ctgctgcgtt aaccaggccg ttctggctga
    57901 gtgagaactg cttcggtacg ggcattgccg gacgctgata atccggtgcc aatgaacaac
    57961 cggttagcgc aagggccaca caaaatggca gaagtttaca aggagacata ggcacataat
    58021 ttctggtgat tttatgccgc caactttacc cgccaggctc tgattttccg gtgacagaaa
    58081 aatgacaaaa ttgtcatttt accaataagc gattgccatc tgatcccgct actctagaat
    58141 tgcccgggca acatgtggag gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac
    58201 cggagaatac ttgaccaaag ggttaaccga agccggtttt gtggtcgatt tggccgacaa
    58261 cgggctgaat ggctaccatc tggcgatgac cggagattat gatctgataa tcctcgatat
    58321 tatgctgccg gacgtgaacg gctgggatat cgtgcgcatg ttgcgttccg ccaataaagg
    58381 gatgccgatt ctgttgctta ccgcgcttgg taccattgaa catcgcgtca aggggctgga
    58441 gttgggggcg gatgactatc tggtgaagcc gttcgctttt gctgaactgc tggcgcgggt
    58501 gcgcactcta ctgcggcgcg gggcggcggt aattatcgaa agtcagtttc aggttgccga
    58561 tctgatggtc gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca tcactctgac
    58621 cagtaaagaa tttactctgc tggagttttt cctccgccat caaggcgaag tgctgccccg
    58681 ctcgcttatc gcctcgcagg tgtgggacat gaatttcgac agcgatacca acgccattga
    58741 tgtggcagtg aagcggctgc gcggcaaaat cgacaacgac tttgagccga agctgattca
    58801 aaccgtgcgc ggcgtgggtt acatgcttga ggtgccggat ggtcagtaag ccatttcagc
    58861 gcccgttttc gctggcaacc cgcctgacct tttttatcag cctggccacc atcgcggcgt
    58921 ttttcgcctt tgcctggatc atgattcact cggtaaaagt gcattttgct gagcaggata
    58981 ttaatgattt aaaagagatt agcgccacac tcaagcgcgt tcttaatcat cccgaagaga
    59041 cacaagcccg acgcttaatg acgctggaag atatcgtcag tggttattcc aatgtgttga
    59101 tttccctggc agatagccag ggtaaaacgg tgtatcactc ccccggtgcg ccggatatcc
    59161 gcgagtttac gcgtgacgcc atacccgata aagacacgcg gggcggcgag gtgtatctcc
    59221 tttccggccc gacgattatg atgccgggcc acggtcacgg gcatatggaa cacagcaact
    59281 ggcggatgat taacttgccg gttggcccgt tggtggacgg caaaccgatt tatacgctct
    59341 acatcgcgct ttcgatcgat tttcatcttc attacataaa tgatttgatg aataaactta
    59401 ttatgaccgc atcgatcatc agcatcctga tcgtctttat cgtgctgttg gcggtacata
    59461 aaggtcacgc accgatccgc agcgtcagcc gtcaaatcca gaacattacc tcgaaagatc
    59521 tcgacgttcg ccttgacccg cagaccgtgc ccattgagct ggaacagctg gtactgtcgt
    59581 tcaaccatat gatcgagcgt attgaggatg tctttacccg ccagtccaat ttctcagcgg
    59641 atatcgctca cgaaattcgc acgccgatta ccaatctcat cacgcaaacg gaaatcgccc
    59701 tcagccagtc gcgcagccag aaggagctgg aagatgtgct ctactctaat ctcgaagagc
    59761 tgacgcgaat ggcgaaaatg gtcagcgata tgctgtttct cgcccaggcc gataacaacc
    59821 agttaatccc cgaaaagaaa atgctcaacc tggcggatga agtcggcaaa gtgttcgatt
    59881 ttttcgaggc attagcggaa gatcgcggcg tggagttgcg gtttgttggt gacgagtgtc
    59941 aggttgcggg cgatccgctg atgctgcgtc gggcgttaag caacctgctt tcgaacgccc
    60001 tgcgttatac gccgcccgga gaggcaattg ttgtgcgctg ccagacggtc gatcaccagg
    60061 tgcaagttac cgtcgaaaac cccggtacgc ccattgcgcc cgagcactta ccgcgattgt
    60121 ttgaccgttt ctatcgcgtt gacccctccc gtcagcgaaa aggcgaaggt agcggcattg
    60181 gtctggcgat agtgaaatcg atagttgtcg cgcataaagg cacggttgca gtaacgtcag
    60241 atgctcgggg gacaaggttt gtgattatgc tgccggagag agagtgatta ttaagttcaa
    60301 attcagggcg tggtgcatca cctcctggta ttgttgatca acaaattaga gaaaaactta
    60361 ttaaacatat gaaatatcga aggcttttat ttttcaaagt ttcacgagat gtttgtatat
    60421 gaaacttagt taaatctgtg tttcttccaa ttgtagatca caatgccctt tctaaatttt
    60481 catcgttata aacaagccat ctatcatata aagcaatgaa taatttaatc gcgattaatc
    60541 atcattaatt gttatattat tcactcaatc tatttatcaa gtgaatttga taaattccta
    60601 atttaaaata tccactattg agccagtatt taacaacgtt aagatgtgca atgtgcagat
    60661 catgttatca gggtggagcg gtagcatata tttcttatgc aatcattcat tttaataatg
    60721 ttaattggat tatttattgc tgtgaataaa aatatattat gaatattggt ttaactttgc
    60781 gtttttgtta ctcgttgtga tatgcgtacc tgtcctgaat aacgccaaga aacaagaggt
    60841 tgagcttgtg attatttttt tgactgaaca gacacccgtt tctgtaaagt aatatttaaa
    60901 caaaattctc acataaccat agcattttct gttccttccc taataacgtc tgaagtcgtc
    60961 ccgcttatca cattatccgc agttgtacgg tacggatcat tattcatttt actggaggtt
    61021 gctatgtcaa ccggattacg tttcacactg gaagtggacg gcctgccgcc ggatgctttt
    61081 gcggtggttt cctttcatct gaaccagtca ctctcttcgc tcttttccct cgatctctcc
    61141 ctggtcagcc agcagtttct ctcccttgaa tttgcgcagg tgctggacaa aatggcctac
    61201 ctgacgatat ggcagggcga tgaagtacag cgccgggtga aaggcgtggt gacctggttt
    61261 gaactcgggg agaacgacaa aaaccagatg ctgtacagca tgaaggtgca cccgccgctg
    61321 tggcgtgccg gtctgcgcca gaacttccgt atcttccaga acgaggacat caaaagcatc
    61381 ctcggcacga tgttgcagga aaacggggtg accgaatgga gtccgctgtt cagcgagccg
    61441 catccttccc gtgagttttg tgtccagtac ggtgagactg attacgattt cctgtgccgg
    61501 atggcggcgg aggaaggcat cttcttttat gaggagcatg cttacaaaag taccgaccag
    61561 agcctggtgc tgtgcgacac agtccgccat ctgcccgaat cttttgaaat cccatggaac
    61621 ccgaacaccc gtaccgaggt gagcaccctc tgcatcagcc agttccgcta cagcgcacaa
    61681 atccgccctt cttccgtggt gaccaaagac tacaccttta aacgccccgg ctgggccgga
    61741 cgttttgaac aggaaggcca gcaccaggac taccagcgca cgcagtatga agtgtatgac
    61801 taccccggac gtttcaaggg tgcccacggg cagaactttg cccgctggca gatggacggc
    61861 tggcgaaaca atgcagaaac cgcgcgggga atgagtcgct cgccggagat atggccggga
    61921 cgacgaattg tgctgacggg gcatccgcag gcgaacctga accgggaatg gcaggtggtg
    61981 gcaagtgaac tgcacggcga acagccacag gcggtgccaa gacggcaggg agcggggacg
    62041 gcgctggaga accattttgc ggtgatcccg gcagacagaa catggcgacc acagccgttg
    62101 ctgaaaccgc tggtcgacgg cccgcagagc gctgtcgtga caggaccggc aggcgaggaa
    62161 atcttctgcg acgaacatgg tcgcgtgcgg gtgaagttca actgggaccg ttataacccg
    62221 gcagaccaag acagttcgtg ctggatccgt gtggcacagg cgtgggcagg caccggtttt
    62281 ggccacctgg cgataccgcg tgtgggtcag gaggtgattg tagacttcct caacggcgat
    62341 ccggaccagc cgatcattat ggggcgcacc taccaccagg aaaaccgcac ccccggcagc
    62401 ctgccgggaa caaagacgca gatgaccatc cgctccaaaa cgtacatggg cagcggattt
    62461 aatgagctga agtttgatga tgcgacaggg agagaacagg tctacatcca cgcgcagaag
    62521 aacatggata ccgaagtgct caacgaccgt accaccaccg taaaacacga tcaccgcgaa
    62581 accgtaaaaa atgaccagac agtcacgatc caggaaggta accgccttct tacggtggaa
    62641 aaaggccaca aaatcacagg cgtactaaaa gggtctttat ctgaggatgt ttttcaggac
    62701 agaggcacga ttgccggttc agtgcatgtt gacgctgtaa acaatggtgg cgaaggcgac
    62761 ggtatacagg cttatacggc gattaaggaa attttgctgg ccgtggagga aagcaaaatt
    62821 gcgctgacgc cggatggcat tcagctacag gtcggggaat cgacggtaat caggctgtcg
    62881 aaggatggca tcaccatcgt gggcggttct gttttcatca actgattcac tcagattatc
    62941 agggagtatt ttatgcagtt cacttttaat gaaggccaca ttcaactgcc gtctcaatgg
    63001 caggatcagt caatgcaggt cctggtgtcc acagataaca gtggcatcaa cctggtcatt
    63061 acccgcgaag ccgttccaca gggaaccctg acaccagagc tgtaccagga aacgctggcg
    63121 ctgtaccagg gcaaactcga tgggtacacc gaacatgcct gccgggaaat cactctggca
    63181 gaggccccgg cctggctgct ggattattcc tggaacggtc cggaagatga agggaatcag
    63241 gggcggatta gccagatagc cgtattccag cggcgtggag atacgctgct gaccttcact
    63301 ttcagcacct ccttatccct gaagaacagt caaaagacga tgctgctgga ggtgataaag
    63361 agcttcaccc cgttgccacc ggagaatgac atccaaaagg accagccgcg ctgacataaa
    63421 cgagggataa cacatgagtg aaggaccagg cgggccacag ggagcgaccg caggcggtac
    63481 gctggcaatg cgaatgctgt cacagcaggc gatggtcgcc agccagatga aacgggcagc
    63541 caacgacaaa gccattgcac agatgttggc atcaaaaaag tctggccccc cgccgccagg
    63601 ctgggcgatg aaattcagca caagagtttt ttgggggcgc tggcaggggc cgtgctgggg
    63661 gcgatagtga ccatcgcaga aggttgcctg attatggccg cctgtgccac cggcccttat
    63721 gcgctggttc tggtgcctgc gctgatgtat gccagctata aggcgagtga ttatgtggag
    63781 gagaaacaga accagcttga atcatggata aacagctttt gtgacacgga cggcgccatc
    63841 aataccggtt ctaaaaatgt aaaaattaac ggagagctgg ccgcacgcgc agcggtcacc
    63901 cttccccctc ctcccccacc aggagcaata cctgaagtcc cacaggggga cccctcatgg
    63961 ggtgatattg ccactgacct gcttgaatcg gcagcggaaa aagcagtgcc actggcgaag
    64021 gcctggggga acgctgttat caccctgacg gacagcaatg ccggtattat ggatcgcgtc
    64081 agcgccggcg catcgcttct gtttcccgcc ggtccggtat taatggagtt tgccaccatg
    64141 gtgggcgggc gtggcgaaat caaaaaagac gtggatttcc cggaagccgg tgaggacacg
    64201 gcgctctgcg acaaggagaa caaaccaccg aggatagccc agggcagcag caacgtcttt
    64261 atcaacaatc agcctgccgc gcgcaagggc gacaaactgg agtgcagtgc ggcaatcgtg
    64321 gaaggttcgc cggacgtctt tattggcggt gaacaggtga cctatctgga tatccagccg
    64381 gagttcccgc cgtggcagag aatgatcctg gggggaataa cgatagccag ctatcttctg
    64441 ccgcccgcgg gactgctggg aaaactgaag aatctggcga aactcggcaa actgggaaac
    64501 ctgctgggga acagcgggaa gctgctgggg gcaaagctcg gcgcgttgct ggggaaaaca
    64561 ggtaagtcgt taaaaagtat tgccaataaa gtcatcagat gggtaacaga tcctgtcgat
    64621 ccggtaaccg gcgcatactg cgacgaacgt accgacttta cgctgggcca gacactcccc
    64681 ctctccttca cccgtttcca cagttctgta ctgccgctgc atggcctgac gggcgtgggc
    64741 tggagcgact cctggagcga atacgcctgg gtgcgtgaac agggaaaccg ggtggatatc
    64801 atcacccagg gaaccacgct gagatttgcc ttcgacggag acagtgatac ggcggttaac
    64861 ccgtatcacg cccagtatat cctgcgccgc cgcgacgatt acctggagct gttcgaccgt
    64921 gatgccctga gcagccgctt cttttatgac gcctttccgg gaatgcgcct gcgccaccag
    64981 gtgaccggcg ataccaatga tgaccgcctg gcacacagcc ccggtgaccg gatgtacatg
    65041 ctgggcggaa tgagtgacac cgccagcaac cgcatcacgt ttgagcgcga cagtcagtac
    65101 cggatcacgg gtgtcagtca caccgacggg atccggctta aactgacgta ccacgccagc
    65161 ggctacctga aagccattca ccgcacggat aacggcatac agacgctggc gacctacgaa
    65221 caggacgaac ggggacggct gacagaagcg gatgcgcggc tggactacca cctgttttat
    65281 gagtacgacg ctgcggaccg gatcatccgc tggtccgata acgaccagac gtggagccgt
    65341 ttcacctacg atgcacaggg ccggtgcgtg accgtcaccg gggcggaagg ctattacaac
    65401 gccacgctgg actatgggga cggctgcacc accgtgacgg acggcaaggg cactcaccgt
    65461 tattactatg atcctgacgg caatattctg cgggaagaag cgccggacgg cagcaccatc
    65521 acgtatgaat gggatgaatt ccatcacctg ctggcccgcc actcccctgc cggacgggtg
    65581 gagaaatttg aatacgccgc cgcacacggt cagttaagcc gttacacggc ggcagacggc
    65641 gtggagtggt agtaccgcta tgatgagcgc ggcctgctca gcaacatcac cgaccctgcc
    65701 gggcagacgt ggacgcagca gtgcgatgaa cgcggcctgc cggtaagcct ggtgtcgcca
    65761 cagggcgaag agacccggct ggcgtatacc gctcaggggc tgctgtcggg gatattccgc
    65821 caggatgaac ggcgtctggg catagagtac gaccaccaca accggccgga aacactcacc
    65881 gacgtgatgg gccgtgaaca ccacaccgaa tacagcggtc acgacctgcc ggtgaagatg
    65941 cgcggccccg gcggtcagtc agtgcggtta cagtggcagc agcaccataa actgagcggc
    66001 cttgagcgca ctggcaccgg cgcggaagga ttccgctacg accgtcacgg caacctgctg
    66061 gcgtacacgg acggtaacgg tgttgtctgg acaatggagt acggcccgtt tgatttgccg
    66121 gtggcgcgaa cggacggtga aggccaccgc tggcagtacc gctacgataa agacacgctg
    66181 caactgacag aagtcatcaa tccgcagggc gagtcatacc gttatcttct ggacaactgt
    66241 ggccgggtga cggaagagcg cgactggggc ggcgtggtct ggcgttaccg ttatgatgcc
    66301 gatggcctgt gtaccgccag ggttaacggt ctggaggaaa ccatcctcta cagccgggac
    66361 gccgcaggcc gcctggcaga agtcatcacg ccggaaggca aaacgcagta tgcgtatgac
    66421 aaatccggca ggctgacggg tatcttcagc ccggacggca tatcacagcg caccggctat
    66481 gacgaacgcg ggcgggtgaa tgtcaccact cagggccgac ggggcattga gtaccactac
    66541 cccgacgaac acaccgtcat ccgctgtatc ctgccaccgg aagatgaaca cgacagacac
    66601 cccggtgaat ccctgctgaa aaccacctac cgctacaacg ccgccggaga actgacggag
    66661 gttatcctgc cgggggatga gacgctgacg ttcagccgtg atgaggcagg acgtgaagtg
    66721 ctccggcaca gtaaccgggg ttttgcctgt gaacagggct ggaatgcagc cggtcagctt
    66781 accagccagc gcgccggatt tttcccggcg gaagccacat ggggcggact gctcccctca
    66841 ctggtacggg agtaccgtta cgacagcgcg ggtaatgtgt cggctgtcac cagccgggaa
    66901 gattacggac gggaaactcg ccgggagtac cggctggacc ggaacggcca ggtcacggcg
    66961 gtgacagcct caggcaccgg gctgggctat ggcgaaggcg acgagactta tggctatgac
    67021 agctgcggct acctgaaggc gcagtctgcg ggcaggcacc agataagtga agagactgac
    67081 ctgtatgccg gaggccaccg gctgaaacag gccggaaaca cgcagtatga ctatgacgcc
    67141 gcaggccgga tggtcagccg cataaaacac cgtgacggct accgcccgga aacagagcgg
    67201 ttccggtggg acagccgtga ccagctgacc gggtattgca gcgcacaggg tgaacagtgg
    67261 gaataccgcc acgacgccag cggcagacgg acggaaaaac gctgcgaccg gaagaaaatc
    67321 cgcatcacgt acctgtggga cggcgacagt attgcggaaa tccgggaata ccgcgatgat
    67381 aaactgtaca gcgtacggca cctggtgttt aacggctttg agctgataag ccagcagtgc
    67441 agccgggtac ggcagccgca tccgtccgtg gccccgcagt gggtgacgcg gacgaatcat
    67501 gcggtgagcg acctgacggg ccgtccgctg atgctcttta acagtgaagg taaaaccgtc
    67561 tggcgaccgg ggcagaccag cctgtggggg ctggcactca gcctgcccgc agacaccggc
    67621 tacccggacc cgcgcgggga actggacgcg gaagccgacc ccggcctgct gtatgcagga
    67681 cagtggcagg atgcggaatc cgggctgtgc tataaccggt tccggtatta cgagccggaa
    67741 acagggatgt acctggtgag tgatccgctg gggttgctgg ggggtgagca gacttaccgg
    67801 tatgtgccga atccgtgtgg gtatgttgat cctttgggat tagcaacatg tcccataatc
    67861 agacaacggg tgctggctaa ccttgaagcc agtcgggcag caagggctgc atctaatttt
    67921 ggtaagaaaa atatctatga ttttatcaag gcaactcaat ctgctatccc tggcacctct
    67981 atcccaaaat cttttgagat tataactgct aatggtactt tctgggtaca cccaaatgct
    68041 acaaaacata tggctgaata tttgactcgg aacggtctct cacattcaac ttctgtagag
    68101 agccaaataa tgttaacaag ttttcacgaa gctgtaagtt tggctagtgc tcaaggtata
    68161 aagtatgaag aaatgatgaa tgttggcggc tgggaactag tatttagtca agctaaatct
    68221 gactcggctt tatcagttat taaacatgct ctttataaat gacgattatg cttacattag
    68281 aaaatgaaac aaactacaaa gatgttgttg ttaatataga tgaatttgta ccttttgata
    68341 taacttttgg aaaggggagc gaacagtgtt tgtactggag aggcggcaat ggtaaaagtt
    68401 ctttggttga agttgggtta aatatgtaca gcggagaagt aagtaacatc acactagcaa
    68461 atatcgattc aggtgaaatt aacattacag atgagatgtt agccaacaac cttcctgaaa
    68521 tacaagggtc tgttatattt cagacaagcg agtggcttaa taaagacatt tccgaatact
    68581 caaacagatt tattgatgat tttgattgca atatcaagtt aataattggt agtaattaca
    68641 ttaaattatt atttaaaaaa gcaacaaatc ccatttgtta tatcattaat aggaatataa
    68701 gatttggttt ttctttgagt agggaacttt cgacattgga aatattagac ctcaatcaag
    68761 aaaaattaga tattctaaag ttatttaaat agttttagtg tttgaaactt accgagagat
    68821 gatagctatg aatgaataca aaaacatcca tcttggagga aacaatggaa tgttaatcca
    68881 ttaaaaaaca tacttcaata tctttagtga agaaagagtt gtatgaaaga aatatatctt
    68941 atgtgagcga catgatgcat gctaattata ttattggcag gaaattttat gatgatagca
    69001 aaaaggaggg gttatttctg taagaatgga tatacccgca gggtttgaac cagacattta
    69061 tttgttgaag tctaagatcc aaatggaagc atttatatcc ctcagatatt atgcgttgac
    69121 gggttgataa agatactttt agagggttaa taaatggaga acgtataata aatctcttaa
    69181 acaaactata acctgtacta atgaaaaaaa tgggaataga taagttaata gcctattcta
    69241 aaaatggtaa agtggtttta cttccaaaat aatggagttt agtatgaaaa tcgaagctaa
    69301 ttcccttact catccctatg actacaacgc ctcactgaac aattatttac attattttga
    69361 gaatttctct cgtaatgact ggaataaaga ttcatgtgga ggatatttta cttttagaga
    69421 tgatgacaga gagtgtgttt tgttttttgt gaattatggt gataattttt cattgcgcta
    69481 tgattgtaca caaagttctg atggaatagt gtcatgctgg tatagtaacc atgaagatga
    69541 taaaatgaat atcatcgtcg atgctgatac agaacaattt atccctttag gttcttgctt
    69601 acctcctgat attactttat cagtaataaa agattttttc aaaaatcctt tagagaaatc
    69661 aaaaaaagta aaatggatga atgcagatga cattgactgg tcatctgtgt attaaagttg
    69721 gattggataa ttatctgttt caaaaatcat taaatttaga acctatctgt tcaacagagc
    69781 tgccttgaac agcaggttct tttatgatgt ccaggagcga atatgcctgt gatgtctggc
    69841 actcagcatc tgactgacct ggactactga tcctactcac acagggagct agacctggaa
    69901 gcaacctact gtaagcagtg cagtagcagc ccacctcaca tcagtccttg caaggtgtgg
    69961 gcctttgtga tcttgctctg tattgctgag atcctcagga aggactcgaa atttaatcag
    70021 gaaggccgtt gctaaaaatc aaaaaataaa ttcaagagca atttctaaaa cttttgaatc
    70081 tactgtatcg gatgaggttt tcactccttt ccaaaaggtg agttttcaag cattcaagtt
    70141 tctgttatga ccaaaacttc accatttaac actaatccga gtattatgtt accatgaaga
    70201 aaaaagatat tagatttgca atatccatta actccaactc aagtaagata gaattaatgg
    70261 aaaaattaaa aacataatcc cagggcttat tgaaataact cccagtgcat taagagaaga
    70321 aaaaacatgg tagttattga agataatgat ctttatgacc ctataaaagc agagggagtt
    70381 gatggatgga tgtattataa attcatatta tccatattcc cattaaaggg agtagacaca
    70441 acattagaat atcaacggga attatctttt cttttcttaa agaaattaaa agatgcgggt
    70501 ctgttaggtg aacttatatg cgaggatgat tttcatgact gatgacctct acatatatcc
    70561 tcagttcgtg gctggtatta catgatctga cagggaaaga tcgtagcgaa atcaaaaaag
    70621 acgtggattt cccggaagcc ggtgaagaca cagcgctctg cgacaaggag actaaaccgc
    70681 cgaggatagc ccagggcagc agcaacgttt ttatcaacaa tcagcccgcc gcgcgcaagg
    70741 gggacaaact ggagtgcagt gcggcgatcg tgggaggttc gccggacgtc tttattgggg
    70801 gtgagcaggt cacctatctg gatatccagc cggagttccc gccgtggcag agaatgatcc
    70861 tggggggaat aacgatagcc agctatcttc tgccgccagc gcagggaaag atcacgtaac
    70921 gctgcttttt tgtactaaat aattcgcact aaatgttcaa taattgagat attccttatt
    70981 acctaaatac agttccacca agattaccta aacccaaatg aggtaaaaga tagtgggtat
    71041 gaataaaaat gaaatccatt tccctatagg atatggttgg ttgaatgaaa aaaaaattat
    71101 tggcaccagt gaatttagcc aattagaacc atggttttat ctagatgaga acaggtgttt
    71161 ttttgcgaat aaaaaatggc caaatgtcat aggtgaacaa ttattcgctt ttgctaaacg
    71221 tcaagataat gatgaattgg cttgtttcaa aattaatgaa tctggagtct gtgagggtgt
    71281 ttatttaatt aatgggtgga ctcctaacgg gtttgatatt atacaatttt accctgattt
    71341 ttgggaatgg gtacatttgg ttatacaaga tgtctcggag tggattgata ataaaggata
    71401 atttgaaaca aaagtaaaaa accgccttca ttgaaggtaa cgtggagtgg tgggcggagc
    71461 agcctgcccg cagacacaga ctacccggac ccgcgcgggg agcgggacgc ggaagccgac
    71521 cccggcctgc tgtatgcagg acagtggcag gatgcggaat ccggactgtg ctataaccgg
    71581 ttccggtatt acgagccgga aaccgggatg tacctggtga gtgatcccct ggggttgctg
    71641 gggggtgagc agacttaccg gtatgtgcct aatcctagtg ggtatgttga tcctctggga
    71701 ttagcaacgt gtcctatact cagacagtgg gcgctgacta accttgaagc cagtcgggca
    71761 gcaagggctg catctaattt tggtaagcct ctggtccagc gaaataaaac gattggcgat
    71821 aaagtaaggg atatgattgc caaagagagg ggaaccactc taattgaaca aaattataga
    71881 gtgacaggag gattaaagcg tattgacgtt gttgatggag taacgggtat agaatctaaa
    71941 gtcggtcgaa cagggttaac aacccgagta cggcaagagg tagcgcggga tattaagatt
    72001 ttgagaagtg agcaacttga tcaaattgaa tgggtgttta caagaagttc aactacaggg
    72061 aagattgggc caactaaacc acttgaagat cttttaaata aacatggtat accgattatt
    72121 tatcgataag gagtgatagt gtgaaactca aaccgattgg tgacttttta aacaaaaatg
    72181 ggtttataaa aaatggtaac aatgattttt ataaagttca tgataagttt gtaaattgta
    72241 taaattttca aaaaaagagt acaggtgata ttttttttat aaacacaggt gttcatcctg
    72301 tgttcgaaaa tttttgcaat gaaccaccga aaaaagaaat agattgttac atccgtaacc
    72361 gtgtgtgtga tggactaagt atatctttgc tgcattcttt tgaaggtagt gagtttgtta
    72421 tcaatgagat aaaaaacaaa gcgttttgtt tttttgaata ctttgattca ttgaataaaa
    72481 tattcgatgc tattcgtgtt gtagatattg aagagagaaa tatttgcgat gagctaagtg
    72541 gtgtaagcag cgtaagatta gctttgatgt gtatgaacta ccagtatgag aaaaaaaaat
    72601 ttagccttgt ttctgatttt gcgaactatg gtcttgctat atctagaggc ccggtaccga
    72661 aaaaagcatt taaggatatg ataaaaataa cagagtgata cctaatccgg tgtaatactt
    72721 gctgcctgtg taccgcgaag attaacggcc gggaggaaac tattctctac aaccgggacg
    72781 ccacaggccg cctggcagaa gtcatcacgc cggaaggcaa aacgcagtat gcgtatgaca
    72841 aatccggcag gctgacgggt atcttcagcc cggacgacac atcacagcgc a
//
LOCUS       DAPOZO010000008        71450 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPOZO010000008 DAPOZO010000000
VERSION     DAPOZO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.008"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            271..2943
                     /gene="rcsD"
                     /locus_tag="ROI21_000575"
     CDS             271..2943
                     /gene="rcsD"
                     /locus_tag="ROI21_000575"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDX5109541.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTTPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYITFRHFS
                     SRSTENVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKTDPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            2960..3610
                     /gene="rcsB"
                     /locus_tag="ROI21_000576"
     CDS             2960..3610
                     /gene="rcsB"
                     /locus_tag="ROI21_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDX5109542.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3696..6545)
                     /gene="rcsC"
                     /locus_tag="ROI21_000577"
     CDS             complement(3696..6545)
                     /gene="rcsC"
                     /locus_tag="ROI21_000577"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDX5109543.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSQKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            6712..8538
                     /gene="atoS"
                     /locus_tag="ROI21_000578"
     CDS             6712..8538
                     /gene="atoS"
                     /locus_tag="ROI21_000578"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HDX5109544.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELNPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            8535..9920
                     /gene="atoC"
                     /locus_tag="ROI21_000579"
     CDS             8535..9920
                     /gene="atoC"
                     /locus_tag="ROI21_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HDX5109545.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            10116..10778
                     /gene="atoD"
                     /locus_tag="ROI21_000580"
     CDS             10116..10778
                     /gene="atoD"
                     /locus_tag="ROI21_000580"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HDX5109546.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKIWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            10778..11428
                     /gene="atoA"
                     /locus_tag="ROI21_000581"
     CDS             10778..11428
                     /gene="atoA"
                     /locus_tag="ROI21_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HDX5109547.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVCAKTEARFEVAADLNTQRGDL"
     gene            11425..12747
                     /locus_tag="ROI21_000582"
     CDS             11425..12747
                     /locus_tag="ROI21_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HDX5109548.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            12778..13962
                     /gene="atoB"
                     /locus_tag="ROI21_000583"
     CDS             12778..13962
                     /gene="atoB"
                     /locus_tag="ROI21_000583"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HDX5109549.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGLVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(14036..14812)
                     /locus_tag="ROI21_000584"
     CDS             complement(14036..14812)
                     /locus_tag="ROI21_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDX5109550.1"
                     /translation="MRKIFLPLLLVALSPVAHGEGVQEVEIDAPLSGWHQAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(14817..16466)
                     /locus_tag="ROI21_000585"
     CDS             complement(14817..16466)
                     /locus_tag="ROI21_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDX5109551.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDMLTTPLGSLWKLYVYAWLEDTHQPEQAYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHISADMWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQAMAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTVRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(16467..20981)
                     /locus_tag="ROI21_000586"
     CDS             complement(16467..20981)
                     /locus_tag="ROI21_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDX5109552.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNSQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTINVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSYSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGDEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLFFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQDSQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VAHVLTMMLLKKAQLKSTVMPQIQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTTQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISINKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(21005..21628)
                     /locus_tag="ROI21_000587"
     CDS             complement(21005..21628)
                     /locus_tag="ROI21_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDX5109553.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFSANEALKIHDSKWLKSNGIASQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(21625..23313)
                     /locus_tag="ROI21_000588"
     CDS             complement(21625..23313)
                     /locus_tag="ROI21_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDX5109554.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGSAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKTQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(23462..26089)
                     /gene="gyrA"
                     /locus_tag="ROI21_000589"
     CDS             complement(23462..26089)
                     /gene="gyrA"
                     /locus_tag="ROI21_000589"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDX5109555.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            26236..26958
                     /locus_tag="ROI21_000590"
     CDS             26236..26958
                     /locus_tag="ROI21_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDX5109556.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(27019..30777)
                     /gene="yfaL"
                     /locus_tag="ROI21_000591"
     CDS             complement(27019..30777)
                     /gene="yfaL"
                     /locus_tag="ROI21_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDX5109557.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVIDSCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMNNNASGGAVFLQQGAEFSLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSDNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     VFRNNIANDGKGGAIYTINNDVYLSEVIFDNNQAYTSTSYSDGDGGAIDVTDNNSNIT
                     HPSGFTIINNTAFTNNSAEGYGGAIYTNSVTAPYLIDISVDDSYSQNNGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGVITKTGSG
                     DLVLNANNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLTLEGDAADLAALQDDPQSIVLNGGVLDLSDFATWQS
                     GTSYNDGLAVSGSSGTVIGSQDVVDLAGGNNMHIGGDGKDGVYVVVDAGDGLISLANN
                     NSYLGTTQIASGTLVVSDNSQLGDTHYNRQVIFTDNQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLQGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNTTDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLRVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTTVVIN
                     PITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPAPDPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERHDHAGGNGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDG
                     EWMLGAVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQ
                     YAWFNNDVSEHEDGVDHYHSSGIIASLEAGYQWLPGHGVVIEPQAQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQTRLGLHSEWRTAVHVTPTLDLNYYYDPHSTKIEEDGSTISDE
                     AVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            31473..33758
                     /gene="nrdA"
                     /locus_tag="ROI21_000592"
     CDS             31473..33758
                     /gene="nrdA"
                     /locus_tag="ROI21_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDX5109558.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            33904..35034
                     /gene="nrdB"
                     /locus_tag="ROI21_000593"
     CDS             33904..35034
                     /gene="nrdB"
                     /locus_tag="ROI21_000593"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDX5109559.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            35034..35288
                     /gene="yfaE"
                     /locus_tag="ROI21_000594"
     CDS             35034..35288
                     /gene="yfaE"
                     /locus_tag="ROI21_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDX5109560.1"
                     /translation="MARITLRITGTQLLYQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(35342..35992)
                     /gene="inaA"
                     /locus_tag="ROI21_000595"
     CDS             complement(35342..35992)
                     /gene="inaA"
                     /locus_tag="ROI21_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDX5109561.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(36073..37263)
                     /locus_tag="ROI21_000596"
     CDS             complement(36073..37263)
                     /locus_tag="ROI21_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5109562.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILGLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     GFNLAFWLCTGVAAVGIILSLIMTVPPVISHADGSTPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLVFFTVGLVVLGYTQGSIMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            37415..38293
                     /locus_tag="ROI21_000597"
     CDS             37415..38293
                     /locus_tag="ROI21_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5109563.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VSILLRDRRKAIQLTAAGHRIVHHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPAVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPNFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVEIGKI"
     gene            complement(38335..39411)
                     /gene="glpQ"
                     /locus_tag="ROI21_000598"
     CDS             complement(38335..39411)
                     /gene="glpQ"
                     /locus_tag="ROI21_000598"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDX5109564.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYSGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(39416..40774)
                     /gene="glpT"
                     /locus_tag="ROI21_000599"
     CDS             complement(39416..40774)
                     /gene="glpT"
                     /locus_tag="ROI21_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDX5109565.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            41047..42675
                     /gene="glpA"
                     /locus_tag="ROI21_000600"
     CDS             41047..42675
                     /gene="glpA"
                     /locus_tag="ROI21_000600"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDX5109566.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVHVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYS
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADTPLPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            42665..43924
                     /gene="glpB"
                     /locus_tag="ROI21_000601"
     CDS             42665..43924
                     /gene="glpB"
                     /locus_tag="ROI21_000601"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDX5109567.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSAPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHTDIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTSTRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAEQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            43921..45111
                     /gene="glpC"
                     /locus_tag="ROI21_000602"
     CDS             43921..45111
                     /gene="glpC"
                     /locus_tag="ROI21_000602"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDX5109568.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            45304..46263
                     /locus_tag="ROI21_000603"
     CDS             45304..46263
                     /locus_tag="ROI21_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDX5109569.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     KGKVEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCY"
     gene            46264..46449
                     /locus_tag="ROI21_000604"
                     /pseudo
     CDS             46264..46449
                     /locus_tag="ROI21_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(46490..47293)
                     /gene="yfaU"
                     /locus_tag="ROI21_000605"
     CDS             complement(46490..47293)
                     /gene="yfaU"
                     /locus_tag="ROI21_000605"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HDX5109570.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGNY"
     gene            complement(47310..48599)
                     /locus_tag="ROI21_000606"
     CDS             complement(47310..48599)
                     /locus_tag="ROI21_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5109571.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKKLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(48656..49861)
                     /gene="rhmD"
                     /locus_tag="ROI21_000607"
     CDS             complement(48656..49861)
                     /gene="rhmD"
                     /locus_tag="ROI21_000607"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HDX5109572.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYREYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFNEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CKLKRPYSH"
     gene            complement(49876..50658)
                     /locus_tag="ROI21_000608"
     CDS             complement(49876..50658)
                     /locus_tag="ROI21_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDX5109573.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(50878..52080)
                     /locus_tag="ROI21_000609"
     CDS             complement(50878..52080)
                     /locus_tag="ROI21_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDX5109574.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLTRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TSGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(52180..52722)
                     /locus_tag="ROI21_000610"
     CDS             complement(52180..52722)
                     /locus_tag="ROI21_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109575.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            53000..53425
                     /gene="nudI"
                     /locus_tag="ROI21_000611"
     CDS             53000..53425
                     /gene="nudI"
                     /locus_tag="ROI21_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDX5109576.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLAHYDLNVATRKTLRLKGLL"
     gene            complement(53464..54066)
                     /locus_tag="ROI21_000612"
     CDS             complement(53464..54066)
                     /locus_tag="ROI21_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDX5109577.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            54374..55513
                     /gene="arnB"
                     /locus_tag="ROI21_000613"
     CDS             54374..55513
                     /gene="arnB"
                     /locus_tag="ROI21_000613"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDX5109578.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DHDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIALQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVNEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            55517..56485
                     /gene="arnC"
                     /locus_tag="ROI21_000614"
     CDS             55517..56485
                     /gene="arnC"
                     /locus_tag="ROI21_000614"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDX5109579.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTKACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            56485..58467
                     /gene="arnA"
                     /locus_tag="ROI21_000615"
     CDS             56485..58467
                     /gene="arnA"
                     /locus_tag="ROI21_000615"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDX5109580.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPEDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHSHAPAAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMHETIDETLDFFLRTVDLTDKPS"
     gene            58464..59354
                     /locus_tag="ROI21_000616"
     CDS             58464..59354
                     /locus_tag="ROI21_000616"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDX5109581.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTLVDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGMPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            59354..61006
                     /gene="arnT"
                     /locus_tag="ROI21_000617"
     CDS             59354..61006
                     /gene="arnT"
                     /locus_tag="ROI21_000617"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDX5109582.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVASQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRNEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            61003..61338
                     /gene="arnE"
                     /locus_tag="ROI21_000618"
     CDS             61003..61338
                     /gene="arnE"
                     /locus_tag="ROI21_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDX5109583.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            61338..61724
                     /gene="arnF"
                     /locus_tag="ROI21_000619"
     CDS             61338..61724
                     /gene="arnF"
                     /locus_tag="ROI21_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDX5109584.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(61718..61984)
                     /gene="pmrD"
                     /locus_tag="ROI21_000620"
     CDS             complement(61718..61984)
                     /gene="pmrD"
                     /locus_tag="ROI21_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDX5109585.1"
                     /translation="MEWLVKKSCCDKQDNRHVLKLCDAGGTIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(62094..63449)
                     /gene="menE"
                     /locus_tag="ROI21_000621"
     CDS             complement(62094..63449)
                     /gene="menE"
                     /locus_tag="ROI21_000621"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDX5109586.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGEHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQLVALVNDEGWYAT
                     RDRGELHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(63446..64408)
                     /gene="menC"
                     /locus_tag="ROI21_000622"
     CDS             complement(63446..64408)
                     /gene="menC"
                     /locus_tag="ROI21_000622"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDX5109587.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDALPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTSLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVSAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVEVDALERLL"
     gene            complement(64408..65265)
                     /gene="menB"
                     /locus_tag="ROI21_000623"
     CDS             complement(64408..65265)
                     /gene="menB"
                     /locus_tag="ROI21_000623"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDX5109588.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSADGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(65280..66038)
                     /gene="menH"
                     /locus_tag="ROI21_000624"
     CDS             complement(65280..66038)
                     /gene="menH"
                     /locus_tag="ROI21_000624"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDX5109589.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFANYSRLY
                     VDLPGHGGSAAIRVDGFDDVTGLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVIVEGGHPGLQNAEQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDEQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(66035..67705)
                     /gene="menD"
                     /locus_tag="ROI21_000625"
     CDS             complement(66035..67705)
                     /gene="menD"
                     /locus_tag="ROI21_000625"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDX5109590.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HCPQNWQELETALADAWRTPTTTVIEMVVNDTNGAQTLQQLLAQVSHL"
     gene            complement(67794..69089)
                     /gene="menF"
                     /locus_tag="ROI21_000626"
     CDS             complement(67794..69089)
                     /gene="menF"
                     /locus_tag="ROI21_000626"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDX5109591.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRVTLFSESSLQHDAIKAKEFIAKLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTLTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(69168..69473)
                     /gene="elaB"
                     /locus_tag="ROI21_000627"
     CDS             complement(69168..69473)
                     /gene="elaB"
                     /locus_tag="ROI21_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDX5109592.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(69528..69989)
                     /locus_tag="ROI21_000628"
     CDS             complement(69528..69989)
                     /locus_tag="ROI21_000628"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDX5109593.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMNKTLET
                     CTRHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            70054..70971
                     /gene="rbn"
                     /locus_tag="ROI21_000629"
     CDS             70054..70971
                     /gene="rbn"
                     /locus_tag="ROI21_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDX5109594.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQALKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            71162..>71450
                     /locus_tag="ROI21_000630"
     CDS             71162..>71450
                     /locus_tag="ROI21_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDX5109595.1"
                     /translation="MVTVVSNYCQLSQTQLCQTFAEKFTVTEELLQSLKKTALSGDEE
                     SIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLA"
BASE COUNT        17522 a        19154 c        17635 g        17139 t
ORIGIN      
        1 aaaaactata acgcttgtta actatttcac aaataattaa catcagcata atttccagca
       61 atcttcgttt atttgcaatt aatttcgttg ggctttttgt aggttatttg tacagcaaaa
      121 tggcgcttgt acatctattt tccccaatgc aggatgataa atatcacggg agaatagaga
      181 atcatcaatc aggtaagagt ctggaatttc acactgtacc ctttatactg ccctatcact
      241 tcgcgaagtt ttaacaggtc ataaacacga atgcgtcaga aagagacaac ggccacgacc
      301 cgcttttcac tcctaccggg gagcattacc cgcttctttt tactgttgat cattgtgtta
      361 ctggtgacga tgggtgtaat ggtacaaagc gccgttaacg cctggctgaa agataaaagt
      421 taccagattg tcgacattac ccacgctatc caaaagcgcg tcgatacctg gcgttacgtg
      481 acctggcaga tctacgacaa cattgccgcg acgaccaccc cctcctccgg cgaaggttta
      541 caagagacgc gcctgaaaca ggatgtctac tatctggaga aaccacgccg caaaacggaa
      601 gcgttaatct ttggctctca cgacaactca acgcttgaga tgactcaacg gatgtccact
      661 tatctggata cattgtgggg cgcagaaaat gtaccgtggt cgatgtatta cctgaatggt
      721 caggataaca gtctggtgct gatctcaacc ctgcccctta aagatctcac ctccggattt
      781 aaagaatcga ccgtcagcga cattgttgat tcacgtcgtg cagagatgtt gcaacaggcc
      841 aacgccctcg atgaacgcga aagcttttct aacatgcgcc gcctggcctg gcagaacggt
      901 cattacttta ccttgcgtac cacgttcaac cagccgggac atctggcaac ggtcgtggct
      961 tttgatctgc cgattaatga tttgatccca ccgggcatgc cgctggacag tttccgcctt
     1021 gagccagacg cgacggcaac gggaaacaat gataatgaga aagaagggac ggatagcgtc
     1081 agtatccact ttaacagtac gaagattgaa atctcctcgg cactcaactc taccgatatg
     1141 cgcctggtct ggcaggttcc ttatggcacc ttactgctgg atacgttgca aaacattctg
     1201 ctgccactgc tgctgaatat cggtttgctg gcgctggcgt tatttggcta tatcacattc
     1261 cgccatttct ccagccgcag tacagaaaac gtccccagca cggcggtcaa taacgaattg
     1321 cgcattttac gggcaatcaa tgaagagata gtctcactgc tgccgcttgg cctgctggtt
     1381 cacgatcagg aatcgaaccg cactgtcata agtaacaaaa ttgccgatca tttgctgccg
     1441 catttgaatc tgcaaaacat caccaccatg gcggaacagc atcaggggat tattcaggcg
     1501 acgatcaata acgagctgta tgagatccgc atgttccgca gccaggtcgc gccgcgcaca
     1561 caaattttca ttattcgcga tcaggatcgc gaagtgctgg taaacaagaa actcaagcag
     1621 gcgcagcgtc tgtatgagaa aaaccagcag gggcggatga cctttatgaa aaacattggc
     1681 gatgcgctga aagaacccgc acagtccctg gcggagagcg cggctaaact caacgccccg
     1741 gaaagcaaac aactggcgaa tcaggctgat gtgctggtgc ggctggtcga tgaaatacag
     1801 ttagcgaaca tgcttgcgga cgatagctgg aaaagtgaga cggtgctgtt ctccgtgcag
     1861 gatttgattg atgaagttgt gccttcagtg ttgcctgcca tcaagcgtaa aggtctgcaa
     1921 ctgctgatta acaatcatct gaaagcacac gatatgcgcc gcggcgatcg cgatgcctta
     1981 cgacgtattt tgctgctact gatgcaatat gccgtgacct caacgcaatt gggaaaaatc
     2041 acccttgagg ttgatcagga tgagtcctcc gaagaccgcc tgacgttccg cattctggac
     2101 accggagaag gcgtaagcat tcatgaaatg gataatttgc acttcccgtt tatcaaccag
     2161 acccaaaacg atcgctatgg caagacggac ccgctggcat tctggctgag cgatcaactg
     2221 gcgcgtaaac tgggcggtca tttaaacatc aaaacgcggg atgggcttgg tacacgctac
     2281 tctgtgcata tcaaaatgct cgcagctgac ccggaagttg aagaggaaga agagcgatta
     2341 ctggatgatg tctgcgtaat ggtggatgtt acttcagcag aaattcggaa tattgtcact
     2401 cgccagttag aaaattgggg tgcaacctgt atcacacccg atgaaagatt aattagtcaa
     2461 gattatgata tctttttaac ggataatccg tctaatctta ctgcctctgg cttgctttta
     2521 agcgatgatg agtctggcgt acgggaaatt gggcctggtc aattgtgcgt caacttcaat
     2581 atgagcaacg ctatgcagga agcggtctta caattaattg aagtgcaact ggcgcaggaa
     2641 gaggtgacag aatcgcctct gggcggagat gaaaatgcgc aactccatgc cagcggctat
     2701 tatgcgctct ttgtagacac agtaccggat gatgttaaga ggctgtatac tgaagcagca
     2761 accagtgact ttgctgcgtt agcccaaacg gctcatcgtc ttaaaggcgt atttgccatg
     2821 ctaaatctgg tacccggcaa gcagttatgt gaaacgctgg aacatctgat tcgtgagaag
     2881 gatgttccag gaatagaaaa atacatcagc gacattgaca gttatgtcaa gagcttgctg
     2941 tagcaaggta gcctattaca tgaacaatat gaacgtaatt attgccgatg accatccgat
     3001 agtcttgttc ggtattcgca aatcacttga gcaaattgag tgggtgaatg ttgtcggcga
     3061 atttgaagac tctacagcac tgatcaacaa cctgccgaaa ctggatgcgc atgtgttgat
     3121 taccgatctc tccatgcctg gcgataagta cggcgatggc attaccttaa tcaagtacat
     3181 caagcgccat ttcccaagcc tgtcgatcat cgttctgacc atgaacaaca acccggcgat
     3241 tcttagtgcg gtattggatc tggatatcga agggatcgtg ctgaaacaag gtgcaccgac
     3301 cgatctgccg aaagctctcg ccgcgctgca gaaagggaag aaatttaccc cggaaagcgt
     3361 ttctcgcctg ttggaaaaaa tcagtgctgg tggttacggt gacaagcgtc tctcgccaaa
     3421 agagagtgaa gttctgcgcc tgtttgctga aggcttcctg gtgaccgaga tcgctaaaaa
     3481 gctgaaccgc agtatcaaaa ccatcagtag ccagaagaaa tctgcgatga tgaagctggg
     3541 tgtcgagaac gatattgctc tgctgaatta tctctcttct gtgaccttaa gtccggcaga
     3601 taaagactaa tcatctgtag gccagataag acgcgtcagc gtcgcatctg gcatttgcac
     3661 tgaatgccag atgcggcgta aacgccttat ccgtcctacg aatcccgcga tttcctgacc
     3721 ctctcggcat ataccgttag cgtctgtttt atcacatcca gcgttaccgg cttcgacagg
     3781 cagctgtcca taccggactc cagacaccgc tgcttctctt cagccaacgc attagcagtt
     3841 actccgatta ccggcaacgt cagtcccaac tgacgaatgc gttgcgtcaa gcggtaacca
     3901 tccatatttg gcatgttgac gtcgctaagc acgatatcaa tatgattctt gctaagtaca
     3961 ttaagcgcat cgacgccatc attcgcggtt ttacattgat agcccaacga acccaactga
     4021 tctgccagaa gacgccggtt aatcggatga tcatccacga ccagaatcat catatcgtcg
     4081 ttatcgctga ccgctttgtc cgtcgacggc agagcgttcg caggatcatc gctctccatc
     4141 tcgatcaaat aaatacgcgc caacaatgcc ggtagctcat gcggggcagc cacactgtgt
     4201 acccactccc ctggcgcttt ctccagcgga ataccaatat ggcgacgaca gaaggtcact
     4261 accgctctac cctgccattt ttggctcact acctcgtcag tgattaacac atcttcggga
     4321 gtcggttcct gcccttcgta tgttgtaacg acgatgccgc tgcgctgcaa actggtttcc
     4381 aggaactgac agagcgacgc attgcggacc gccagccagc agcgtttacc actcaaccct
     4441 tccacgcctt ttttctgcgg atactgagcg ccgtacaacg gaatacgcac ggtaaactgg
     4501 ctgcccattc ccggttctga atctaccgag atatcgccgt ccatcatgct gatcagtttt
     4561 tcacaaatcg ccagacccag accggtcccc tggaagtttc gctgtacgcc cgttccgacc
     4621 tggaagaagg gatcaaacaa gcgcaccact tctttcgccg gtatccccac gccggtatcg
     4681 cgaacacgga tagagagata atcgccatca gcgcgaacat gcaaaactat acagccggta
     4741 tcggtgaatt ttatggcgtt actcaacagg ttggagatga cctgctgtaa acgcatcggg
     4801 tcgccattta aggccactgg cacatccggt tcaataaagc agtacaagcc taactgcttg
     4861 cgtaccacca gcggtaaata gttggcggtg atgtggttca tcacttcacg cggtgaaaac
     4921 tcacgcggtt cgatcttcaa ctgttctgat tcaattttcg agaaatcgag aatatcgctg
     4981 ataattttca acaacaggct ggaagagttg ttcattgccg tcaccagacg atcgacgcct
     5041 ttcggtaact ctttggtttg caacagatcc aggttaccga taatgccata cagcggcgtt
     5101 cgcagctcat gactgacggt ggcaaggaac atcgattttg actggctcgc ctgttccgct
     5161 gcttgtgcca tctcctgcaa cgactcttcc atcttcacgc gcgaagaaac atccaccagc
     5221 acacaaatgg ccacgttttc attacgatag cgcgaatgga cgaagctgat ttgcagattg
     5281 gtattgttgc tggtcaggac atcaacaaaa ttgacctgct gcccacagat aatttgcgtc
     5341 agtcgttggc gatcctcatg cgtaagcata ttgagatagg tatgcgccag ttcgttactt
     5401 aaaatattga cgccatcagc ggtacgcaaa atgcagatac ccactggcgc ggaggcgaca
     5461 atcttgcgat taaactgctc atgttcttcc agtcgcaggg cgtcgctttc cgccggaatg
     5521 aaaatacgtc gctcgtacat ccgcgcgaga gtaaacaatg cagctccggc aagcacattc
     5581 agcaaaattg cgttaaggat caacatgcga atgcgttcca gcaccttatc aaccggcacc
     5641 gaatacacga tgcttagcga tgagggtggc agatttttct tcagcaccag ctcccggaac
     5701 ccttccgtat agccaaacca ggagcgttcc tgcatccagc gaggatcgcc cttaatttta
     5761 ctttctggtc cggtaagcga aatcagggta tgaccatttt catcaagaat ggtaaccccc
     5821 atcggcaacg taccaggtaa gaaaaagttc tccatccgga tggtctgctc gacacccaaa
     5881 agcgcctgca accggttcgc cagataaact ggcgtcaacg cgtaaaaata cccgacgcca
     5941 gggcgcggac cttcgctgat ccagtagagg ttactgccgc tatcatcttg tggtgcattt
     6001 cgatatttat tgatgcgttc atgcaacgct ttcaacgcgg tatcgcgttc cactggcata
     6061 tcacgcagac cgaaattggc catgcagagg ttatcgctgc cgattaaaaa tacccggttg
     6121 agatcgtaag ccgcagagaa attatcgcgc cagtagcgca taaaccacgc caatgactcc
     6181 agagaacctc gccaggtgtt actcattgcg gaacaatcgg agtcggcaaa cagcggttca
     6241 aacgcaggca catccgcctg cgtttctcgt ccacgcgggg aaagcacacc gttttctgcc
     6301 gataagcgat tttcggcgat gtacttcagc tctttcatca catcagaggt gcgttgaata
     6361 aagcgctgag cctgatcgga actcagatta aattcctgac gaatttccga ttctcgctga
     6421 tgtaacgcat taacgatgta aaaaacggat gaaaaagcaa tcaacagcca gagcactaac
     6481 gccaatgctc tgaacatgta gcgcgaggct ttcagggttg tacgaaaaga agcaaggtat
     6541 ttcaaagggg cgaagctccg cctcaggtga ccgatggagt gtggttaagg tagcggtaaa
     6601 agcgtgttac cgcaatgttc tctcttctct ggaatatgat acaccgccga gaaatcatca
     6661 ccttaacctc tgataatcgt catataccgg acaagactag tggatttcag catgcattat
     6721 atgaagtgga tttatccacg ccgcttacgc aatcaaatga tcctgatggc aatcctgatg
     6781 gtcattgtcc caacgcttac tattggttat atcgtagaaa cggaaggacg ttcagcagtc
     6841 ttatctgaaa aagagaaaaa actttctgcc gtggtcaacc tgcttaatca ggcactaggc
     6901 gatcgctatg atctctacat cgacttacca cgtgaggagc gtatccgcgc attaaatgca
     6961 gaacttgccc ccattaccga aaatatcact cacgccttcc ctggcatcgg cgctggttat
     7021 tacaacaaaa cgctggatgc gataatcacc tacgcacctt cagcgctata tcagaataat
     7081 gtgggcgtta ccattgccgc agatcaccct ggtcgcgaag tcatgcgtac aaatacccct
     7141 ttggtctatt caggcaggca ggtgcgcggc gatattttga attcaatgat tcccattgag
     7201 cgtaatggtg aaatcctcgg ctatatctgg gccaatgaat taaccgaaga tattcgccgt
     7261 caggcctgga aaatggatgt gaggattatc attgtgctca ccgccggttt gctgataagc
     7321 ctgctgttga ttgtcctttt ctcccgtcgc ctgagcgcca atattgatat catcaccgat
     7381 ggcctctcga ctctggcaca aaatattccc actcgattac cacaattgcc cggtgaaatg
     7441 gggcaaatca gtcagagtgt taacaacctc gcccaggcac tgcgtgaaac gcggacactc
     7501 aacgatctga ttattgaaaa cgctgccgat ggcgtcattg ccattgatcg ccagggtgat
     7561 gtaaccacca tgaacccggc agcagaagtc atcactggct atcaacgtca tgaactggta
     7621 gggcagcctt actccatgtt gttcgacaat actcagttct acagtccagt actggatacg
     7681 ctggaacatg gcaccgaaca tgtagcgctg gagatcagtt ttccaggccg tgaccgcacc
     7741 attgaactca gtgtcactac cagtcgtatt cataacacgc atggtgaaat gataggtgct
     7801 ttggtgattt tctctgattt aactgcccgc aaagaaaccc agcgccgcat ggcgcaagca
     7861 gaacgcctcg ccacactggg tgagctgatg gctggcgtcg cgcatgaagt gcgtaatccg
     7921 ctaacggcta ttcgtggtta tgtacagatc ttgcgccaac aaaccagcga cctaatacat
     7981 caggaatatc tgtccgtagt actcaaagaa atcgattcaa ttaacaaagt tattcagcaa
     8041 ttgctcgaat tttcacgtcc acgccacagt caatggcaac aagtcagtct caatgcattg
     8101 gttgaagaaa ctctggtact ggtacaaacc gccggcgtac aagcgcgggt cgacttcata
     8161 agcgaactgg ataatgaatt aaacccgatt aacgccgatc gtgaactgct caaacaggta
     8221 ctactgaata tcctgatcaa tgccgtccag gctatcagcg cacgagggaa aattcgcatt
     8281 caaacctggc aatacagcga ctcacaacag gccatttcga tagaggacaa cggctgtggc
     8341 attgatctct cgctgcaaaa aaagatcttc gatccctttt tcaccaccaa agcctcagga
     8401 accgggcttg gtctggcgtt aagtcaacgc atcattaatg cccatcaggg tgatattcgc
     8461 gtcgccagtt tgccgggcta cggcgcaacc ttcacgctta ttttaccgat caatccgcag
     8521 ggaaatcaga ctgtatgact gctattaatc gcatccttat tgtggatgat gaagataatg
     8581 ttcgccgtat gctgagcacc gcttttgcac tacaaggatt cgaaacacat tgtgcgaaca
     8641 acggacgcac agcattacac ctgtttgccg atattcaccc tgacgtggtg ttgatggata
     8701 tccgcatgcc agagatggac ggcatcaagg cactcaagga gatgcgcagc catgagacac
     8761 ggacccccgt tattctgatg acggcctatg ctgaagtgga aaccgccgtc gaagcgctac
     8821 gctgcggggc cttcgactat gttatcaaac cgtttgatct cgatgagttg aatttaatcg
     8881 ttcagcgcgc tttacaactc cagtcaatga aaaaagagat ccgccatctg caccaggcac
     8941 tgagcactag ctggcaatgg gggcacattc tcaccaacag cccggcgatg atggacatct
     9001 gcaaagacac cgccaaaatt gccctttctc aggccagcgt cttgattagc ggtgaaagcg
     9061 gcaccgggaa agagttgatt gccagagcga ttcactacaa ttcgcggcgg gcaaaggggc
     9121 cgttcattaa agtcaactgc gcggcgctgc cggaatcgtt gctcgaaagt gaactgtttg
     9181 gtcatgaaaa aggtgcattt actggtgcac aaaccttgcg tcagggatta tttgaacgtg
     9241 ccaacgaagg tactctgctc ctcgacgaaa ttggcgaaat gccgctcgta ctgcaagcca
     9301 aattactacg cattctgcag gaacgggaat ttgaacggat tggcggccat cagaccataa
     9361 aagttgatat ccgcatcatt gctgccacca accgcgactt gcaggcaatg gtgaaagaag
     9421 gcaccttccg tgaagatctc ttttatcgcc ttaacgttat tcatttaata ctgccgcctc
     9481 tgcgcgatcg ccgggaagat atttccctat tagctaatca ctttttgcaa aaattcagta
     9541 gtgagaatca gcgcgatatt atcgacatcg atccgatggc aatgtcgctg cttaccgcct
     9601 ggtcatggcc gggtaatatt cgagagcttt ccaacgtcat tgaacgcgcc gtcgtgatga
     9661 actcaggccc gatcattttc tctgaggatc ttccgccgca gattcgtcag ccagtctgta
     9721 atgctggtga ggcaaaaaca gcccctgtcg gtgagcgtaa tttaaaagag gaaattaaac
     9781 gcgtcgaaaa acgcatcatt atggaagtgc tggaacaaca agaaggaaac cgaacccgca
     9841 ctgctttaat gctgggcatc agtcgccgtg cattgatgta taaactccag gaatacggta
     9901 tcgatccggc ggatgtataa cgccaaaact tgctatgcag aaatttgcac agtgcgcaat
     9961 tttctgcata gccgctcatt ctccttataa atcctcatcc aatttatccc ttcatattca
    10021 attagttaaa taacaaaacc caataatctc attctggcac tccccttgct attccctgac
    10081 tgtacccaca acggtgtatg caagagggat aaaaaatgaa aacaaaattg atgacattac
    10141 aagatgccac cggcttcttt cgtgacggca tgaccatcat ggtgggcgga tttatgggga
    10201 ttggcactcc atcccgcctg gttgaagcat tactggaatc tggtgttcgc gacctgacat
    10261 tgatagccaa tgataccgcg tttgttgata ctggcatcgg tccgctcatc gtcaatggtc
    10321 gagtccgcaa agtgattgct tcacatatcg gcaccaaccc ggaaacaggt cggcgcatga
    10381 tatctggtga gatggacgtc gttctggtgc cgcaaggtac gctaatcgag caaattcgct
    10441 gtggtggagc tggacttggt ggttttctca ccccaacggg tgtcggcacc gtcgtagagg
    10501 aaggcaaaca gacactgaca ctcgacggta aaatctggct gctcgaacgc ccactgcgcg
    10561 ccgacctggc gctaattcgc gctcatcgtt gcgacacact tggcaacctg acctatcaac
    10621 ttagcgcccg caactttaac cccctgatag cccttgcggc tgatatcacg ctggtagagc
    10681 cagatgaact ggtcgaaacc ggcgagctgc aacctgacca tattgtcacc cctggtgccg
    10741 ttatcgacca catcatcgtt tcacaggaga gcaaataatg gatgcgaaac aacgtattgc
    10801 gcgccgtgtg gcgcaagagc ttcgtgatgg tgacatcgtt aacttaggga tcggtttacc
    10861 cacaatggtc gccaattatt taccggaggg tattcatatc actctgcaat cggaaaacgg
    10921 cttcctcggt ttaggcccgg tcacgacagc gcatccagat ctggtgaacg ctggcgggca
    10981 accgtgcggt gttttacccg gtgcggccat gtttgatagc gccatgtcat ttgcgctaat
    11041 ccgcggcggt catattgatg cctgcgtgct cggcggtttg caagtagacg aagaagcaaa
    11101 cctcgcgaac tgggtagtgc ctgggaaaat ggtgcccggt atgggtggcg cgatggatct
    11161 ggtgaccggg tcgcgcaaag tgatcatcgc catggaacat tgtgccaaag atggttcagc
    11221 aaaaattttg cgccgctgca ccatgccact cactgcgcaa catgcggtgc atatgctggt
    11281 tactgaactg gctgtctttc gttttattga cggcaaaatg tggctcaccg aaattgccga
    11341 cgggtgtgat ttagccaccg tgtgtgccaa aacagaagct cggtttgaag ttgccgccga
    11401 tctgaatacg caacggggtg atttatgatt ggtcgcatat cgcgttttat gacgcgtttt
    11461 gtcagccggt ggcttcccga tccactgatc tttgccatgt tgctgacatt gctaactttc
    11521 gtgatcgcgc tttggttaac accacaaacg ccgatcagca tggtgaaaat gtggggtgac
    11581 ggtttctgga acttgctggc gtttggtatg cagatggcgc ttatcatcgt taccggtcat
    11641 gcccttgcca gctctgctcc ggtaaaaagt ttgctgcgta ctgccgcctc cgccgcaaag
    11701 acgcccgtac agggcgtcat gctggttact ttcttcggtt cagtcgcttg tgtcatcaac
    11761 tggggatttg gtttggttgt cggcgcaatg tttgcccgtg aagtcgcacg ccgagttccc
    11821 ggttctgatt atccgttgct cattgcctgc gcctacattg gttttctcac ctggggtggc
    11881 ggtttctctg gctcaatgcc tctgttggct gcaacaccgg gcaacccggt tgagcatatc
    11941 gccgggctga tcccggtggg cgatactctg ttcagtggtt ttaacatttt catcactgtg
    12001 gcgttgattg tggtgatgcc atttatcacc cgcatgatga tgccaaaacc gtctgacgtg
    12061 gtgagtatcg atccgaaact actcatggaa gaggctgatt tccaaaagca gctaccgaaa
    12121 gatgccccac catccgagcg actggaagaa agccgcattc tgacgttgat catcggcgca
    12181 ctcggtatcg cttaccttgc gatgtacttc agcgaacatg gcttcaacat caccatcaat
    12241 accgtcaacc tgatgtttat gattgcgggt ctgctgctac ataaaacgcc aatggcttat
    12301 atgcgtgcta tcagcgcggc agcacgcagt actgccggta ttctggtgca attccccttc
    12361 tacgctggga tccaactgat gatggagcat tctggtctgg gcggactcat taccgaattc
    12421 ttcatcaatg ttgcgaacaa agacaccttc ccggtaatga ccttttttag ttctgcactg
    12481 attaacttcg ccgttccgtc tggcggcggt cactgggtta ttcagggacc tttcgtgata
    12541 cccgcagccc aggcgctggg cgctgatctc ggtaaatcgg taatggcgat cgcctacggc
    12601 gagcaatgga tgaacatggc acaaccattc tgggcgctgc cagcactggc aatcgccgga
    12661 ctcggtgtcc gcgacatcat gggctattgc atcactgccc tgctcttctc cggcgtcatt
    12721 ttcgtcattg gtttaacgct gttctgacgg caaccctaca gacagaagga ataaaaaatg
    12781 aaaaattgtg tcatcgtcag tgcggtacgt actgctatcg gtagttttaa cggttcactc
    12841 gcttccacca gcgccatcga cctgggggcg acagtaatta aagccgccat tgaacgtgca
    12901 aaaatcgatt cactacacat tgatgaagtg attatgggta acgtgttgca agccgggttg
    12961 gggcaaaatc cggcacgtca ggcactgtta aaaagcgggc tggcagaaac ggtgtgcgga
    13021 ttcacggtca ataaagtgtg tggttcgggt cttaaaagtg tggcgcttgc cgcccaggca
    13081 attcaggcag gtcaggcgca aagcattgtg gcggggggta tggaaaatat gagtttagcg
    13141 ccctacttac tcgatgcaaa agcacgctct ggttatcgtc ttggagacgg actggtttat
    13201 gacgtaatcc tgcgcgatgg tctgatgtgc gccacccatg gttatcatat ggggattacc
    13261 gccgagaacg tggccaaaga gtacggaatt acccgagaaa tgcaggatga actggcgcta
    13321 cattcacagc gtaaagcggc agccgcaatt gagtccggtg cttttacagc cgaaatcgtc
    13381 ccggtaaatg ttgtcactcg gaagaaaacc ttcgtcttca gtcaagacga attcccgaaa
    13441 gcggattcta cgactgaagc gttaggcgca ttgcgcccgg ccttcgataa agcaggaaca
    13501 gtcaccgccg ggaacgcgtc tggtattaac gacggtgctg ccgctctggt gattatggaa
    13561 gaatctgcgg cgctggcagc aggccttacc cccctggctc gcattaaaag ttatgccagc
    13621 ggtggcgtgc cccccgcatt gatgggtatg gggccagtac ctgccacgca aaaagcgtta
    13681 caactggcgg ggctgcaact ggcggatatt gatctcattg aggctaatga agcatttgcc
    13741 gcgcagttcc ttgccgttgg gaaaaccctg ggctttgatt ctgagaaagt gaatgtcaac
    13801 ggcggggcca tcgcgctcgg gcatcctatc ggtgccagtg gtgctcgtat tctggtcaca
    13861 ctattacatg ccatgcaggc acgcgataaa acgctggggc tggcaacact gtgcattggt
    13921 ggcggccagg gaattgcgat ggtgattgaa cggttgaatt aatcaataaa aacacccgat
    13981 agcgaaagtt atcgggcgtt ttcttgaaca tcgacggcga aggtaacccc attaatcacc
    14041 agtcaaaact tttcaccagc gtcagctcgc cagcattacg catcggtaca ataaatgttt
    14101 cctgtttctc attgaccgat ccttcatcgg tgatcaacgt cagttgggcg gtcgtcaatt
    14161 ccgtttcgct gcgtccgcca tagtagttga tatacacctg ataacgtccg tgaactggcg
    14221 ctggcatggc gaaaatctcg ggtccgtacc ccgtcgtgac atccatatcc agtgcaccac
    14281 tgtttttcag cacggtgtta ccgtaccagg cgtgttcgcc atcgggcgta acaacgtgaa
    14341 ggtcgagatc ggtattgtcc gtgtcccagg agagaaccag ccgtaaacgc gcgcggatcg
    14401 ctccagcgcc cggcgttgag taaaactgca ttttttgtcg gctttgccca tccgggctga
    14461 tgacctgcac gctgttgctg ccttcagtga aaatataagg gcgtgcaaat gaaccatcgg
    14521 attcaattct ctgcggcata ctggcaccgt tgaccaccag ccggccctgc tgaactttac
    14581 ccgccgcagc ataattcttt attttgccgc ggatctgcgc tgagatattt tgatcatctg
    14641 ccatgttgac ggacgacgcc gggtaattga tcgattgtga aaaactggca tcttcgccct
    14701 ctgcctgatg ccagccagag agcggtgcat caatctcgac ttcctgcaca ccttcgccat
    14761 gggcaacagg cgaaagcgcc accagtaaca acggaagaaa aatctttcgc atagcattag
    14821 tccattaata attgtcgggt cagcgtttcg atatagttct catcaagccc ggtgggataa
    14881 ccatcaaagg ccagatgcag atactcatgg gttaaatcga gccgatcctg aagcgtaaag
    14941 aagttacgaa taaacagccg tttctgctgc cgaacggtat aggggaaacc tgagactaaa
    15001 cggcaaaccg caaacacgtc tggttcgttg taccccgtct caccttgtaa tatacgccgc
    15061 cactgcggca tttttttcgc cagccaggct ttcgctttgg gtaataactg gcaggttgag
    15121 cgtggcgctc cccagcggct aaggctgttg tcgggataag caaacgcgag tatctggtcg
    15181 taacgctccc cctgccccgc ctgggccatg gcttgccgcc aggaaagcgt cccttcggta
    15241 gcgcgactgc cgtgataatg aaccggatcg cccgcataga tgagatcctg cgtccaggcg
    15301 gtcatcgttc gcgcccccgt ggtcgctggc gaagcagaaa cacgctgcgt ggcgctactg
    15361 tcggggatgg tcaggcaatc gccttcccga ttggcgtttt gctgtaaaaa agtgcgaatc
    15421 gccacggtca tggctttcgc tgcctcaggg ggcgtacttt tcgcctcgcg atccagaact
    15481 ctggcaacgt actcttcacg atcaagatgc gactgcaatt tcagtttgcc cttttcgctc
    15541 agtaaggtcg tctcaccgtg gctgacaaac gtaatgtggt taccattagt aaacgtcaca
    15601 cgatagcgcc cattaagtac gccgggtttt actgccgtcg tactcttttc cgctgtgatt
    15661 ttcttcagcg gatagcgggc aaacagttcc acctctacac attgcccact ggcaacctgc
    15721 gtcggcactg gcaaaactcg attaagcacc gtcgcataac gggtcagtac cgttttgctg
    15781 gtgccactgc cagtaaccca cagcggtgta ccatcggtta accagccggc aaaaccaccc
    15841 tgacggattt cctgtttatc atcggcaaac cagctccagg ttttcacccg tacccgacta
    15901 ccgagcatgg atgcgacacc gattttcgcc tcgtccagca ccacatccag taagacttcc
    15961 tgagctttgt tttgcgcagg taacgtcgct aatgaatcaa gcagcgattt aaccgttacc
    16021 gaggtttctg gcttcagcgt agtaagagac gctaaccacg caggtgcctg acgctgttgc
    16081 cagtattgcc cccacatatc tgcgctaata tgcaaccgct gcggcgcaaa atagagccca
    16141 caggaacgta ccagcgcggt atcgcgcgtt atgctctccc cagcctgaca acaataaacc
    16201 tcttccgggg agtttccacg acactggtag gcctgctccg gttgatgggt atcctccagc
    16261 caggcgtaaa catacagttt ccacaaactc cctaacggcg tggtgagcat gtcaggtaat
    16321 gctgagactt tggtcacgcc tgatgacgac agctgataca gttgatcgtg ctgcgcaccg
    16381 cgcagcacca gttgtaacgg cgtctcctcc gcaagcgttg gcagagtaac caacgccaat
    16441 agccagacga ttctgcgcca gttcacttat ttcacctgca tcccggtcca ttcactccct
    16501 gccgcaacac tttgctgtgc aggtgcatag gaacgcacat aacgagcagg aggcagaacg
    16561 aattgccctt tttgcgagaa gcgcagcaaa tggcggaaag tgaccgttcc cgtcagttct
    16621 ttcaccggca ccatataggc cagttcgccc atttcatttc gcgctttttc cagcaattgc
    16681 ccctgctgtt tcgcggcgtt gggtttattg atcgaaatgc cccatgttgt gcgctcaaca
    16741 tcggctcccg gcgggagcgg tacttccacc tgaccgtagc gcagaacggc atcctgctcg
    16801 ctggtaagcg taatttcatc gagatacagc gcatcgctgt caatctcatt gctggtcacc
    16861 ggctgcagag taaagctcat ctcttcttca ccggggataa gccgatacaa ctggcgttca
    16921 acggtcaccg gaatgttact ttgttgagtc gtttttgccg gttcacgcca gcggacctgc
    16981 acattttgcg gcgataattc gtcaccaaaa gagagaatgt ccggcacgcc ctgaccaacc
    17041 caacgccagt attcgccccc tccagttaac ttatgtttgg cccatgcgcc cgcaggcgca
    17101 ggcaaaacaa ctggaggcat tgtcgccata tatttcgcca gccagttcat ggccagcgcg
    17161 cgttcaatag tggattgctc agcggtcaaa ccacttaaaa tagcagccgc ctgggtagcg
    17221 tcgcccccgc tattgagcag cacaactgtg tgcgccagcg gctgattgct gtttgccgct
    17281 ttatcccacg cgtattgctg aatctgtggc atcacagtgg acttcaactg cgcttttttc
    17341 agcaacatca tggttaagac gtgtgccacc gcatctgcca gtggagattc cggtgtatcg
    17401 aggatcaggc tgtcattgat atcgcgggtg tcttcctcag agaaatcttc agttttagtt
    17461 ccgcgccggg cgatagcttc atccaaccct ttcaacaacg ttttgcacgg cagattcatc
    17521 tcctgtgccc acgccagcac cagcgcgcga tgcaataacg gcatgttatc tgcctgctcc
    17581 gcgtagctgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    17641 ctgtcctgcc agtcggcgta ccatgcccat gccgtcagga aggcgtcgcc attgccatct
    17701 tccccccacc aggtaaagcg cgcaccgggc cccgccagtt gcatcagccg cagacggtta
    17761 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    17821 caggcgagac tgagcgggat cagacggcta ccggtgttga tcacgccacc ccacggttca
    17881 tcgactaacg catcaagatt gttacggaaa atctcctgcg gcgtttcgct actttgtagc
    17941 cggatattgc tcgcctgctc gggcaacatc agcgcgttat cgccaccgcc gagcatgaca
    18001 tttttctgct gttcaacggg ccagctgtta tccacaaaaa acagttttgt gctaatgctg
    18061 tcctgcactt gcccattttg ttgcagttct gcgcttaaca agccagattg ctgaatgttc
    18121 tgcgccagcg aaatataatt cgcccctttg tgcagtgtca gcgtctggcg catctcgtcg
    18181 cctgcaaact tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    18241 cctgctgccg gtttgtcgcc catgcgatac accgttggca tactccactt catgtagaga
    18301 tttttttccg aacgaagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccct
    18361 ctggctgtaa tgcgccaacg ggttaacgaa tcaggcatca ggaacgtaaa atacgctttg
    18421 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    18481 ggacgttcaa gcatttttac tcgccgctcg ctgcggttgg tcgcgccagg cgcaaccggc
    18541 tcgctggaga gcgcctggtc gtagctgata aacgacagac tggagctggt acgcacattg
    18601 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttccgg ttgcagcgcg
    18661 tagatcattt catcgaccac gccaaccgtt agctgcgcag aaacaggttt acctttcagc
    18721 gacgaggtta attcgacatt gaccagttca ccgggctggt aatgggtttt gtccgttttc
    18781 acccggatat ccagctgggg aacggcaact ttgatcccgg cgttctgaaa actgtactga
    18841 ccgttacggg tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcaccggt
    18901 acgcgggctt catactgggt atcgtttaaa cgttgtaacg tcagccagtt tgccggatgc
    18961 gaaagcagcg actgctgttc aacgcgatcg cgttccagcg tcaataatgc ttcatcaatt
    19021 ggctccggga aggtaatcag catcttcgcg gtttcgccag gctggtacag cgttttatct
    19081 gccacgatat ctaccgtacc agtatgcgac gtgctgccct taccgctgac tgcatggctt
    19141 aacccggcga gaattaagcc gtctttatcg cgtaacgtca gattgtagtt acccggttta
    19201 tcgaaattga cggtaaagga tttgccacca gaaggtagat ctccgctgtg gctcgtgcgg
    19261 tcttcgagac gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca
    19321 taacggaaca caaccgactc gccgctataa ctgtattgtg cggcggtact taaagagtaa
    19381 tgcgccaggc cgcgttcaat gaggatctct ttggtagtgg taactcgata cgccgcgccg
    19441 tcactggcgg agacggttaa caaataacgg cttggtttat cagcggcggg gagatttaac
    19501 gccacatgac cgctatcgtc ggacaccgtt tcactgcctt ccagcgacac cgggaaacgt
    19561 ccggcataac gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc
    19621 acacgggcat cttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc
    19681 gcttcgccag ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg
    19741 atatagttgg ctacgcgaaa actactgcta tagacctgat tgcggtaagc aagacgtaac
    19801 tcataacctc cggcaacggc attttctggc aggcggaaac tcccctgccc gccattacgc
    19861 gcatccagcg tgacattgat ggtttgcaac agactgccgt tggcgtccag cactgaaagc
    19921 ttcgccgggg cgctaacgat gggggatgaa tgcaacgggt cgtggaactc gcggcccatc
    19981 actttgacat caacacgatc gcctgcgcga tacagagggc ggtcggtgaa gatatacaag
    20041 cgggtgttgt aaatttcgct ttcgtagaaa aaattctcgg agacaaaaac gccgccttca
    20101 gcatctttac ccagaatgta tgaacgttcc ggcgatatat gttgtaactg caaggtaccg
    20161 ctgtcatcgg tcacaccgcg ggtcatcacg ccaagaccgt cagtccacaa gatctctgag
    20221 ccgggcttcg cttcaccctg ttttttaccc gcggtccaca ccagaagctc gttacctgac
    20281 actttgctaa gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacca
    20341 accatcgcct cgacgaggta cagtcccggc tcttgtttgc cgagaggaat atagatgttg
    20401 cccggctgcg gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc
    20461 tcgaccggtt tagcctgcca taatgggtaa cggaactgtt ccaccagtgg atattttttc
    20521 agcggggaaa actggctgtt ctgtacataa cgggaaggtt taataatggc attaccgagc
    20581 tgtaattcgg gtaacgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc
    20641 atcacgcggc gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc
    20701 ccgtcgccca aatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa
    20761 aatgccatcg ggtcaggaat acgatacagg cgaacgtcca cgccgccgta ctcttccatc
    20821 tgatagcgtc gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg
    20881 ctaaaactgc tgtcagcgag caaaaagaat gttcccccgg cgggcggcgc atagttgctg
    20941 gaaggaagcg aatcatcagc attggcaagc cctgttccca caagagacag ggacaagcac
    21001 gctgctatcg cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt
    21061 tgggtatcca tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc
    21121 cgttgtcagt tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca
    21181 ttaagtgctg ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta
    21241 tgtcctggcc aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc
    21301 cggttttccc gttcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg
    21361 tcatttcagg cggcaaatac tgacttgcta taccgttact ttttagccat ttgctgtcgt
    21421 gaattttcag cgcctcgttc gccgaaaatc gcaccaggcc cgcacaatcc tgctgatacc
    21481 agcgcggact tggcccctgg cggagctgtt cctgtgcaat gcgcacaaac caggcgcgaa
    21541 acagccccga ctgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc
    21601 aaatcagcgc cagcagcccg tgcctcatag cggctgccag gttatgggta gccactgcca
    21661 ggcagcaccg ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc
    21721 gtccagcttc ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt
    21781 cggcagactg gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata
    21841 gagcggaacg atgccatcag tgggtattac atccaccatt gccggacggg ttttattcag
    21901 tgtttgcagg gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg
    21961 catcgccagc gacacgcgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg
    22021 tgttttcgga tactgaccgt atcgggaact cacttcgcga cgccagattt gcgcttcgcc
    22081 ctgctgagtc tggtttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat
    22141 attttgcgta aacagtttcc caggcagttg tgcctgttcg gctgagccat caaattgacc
    22201 aacaaataac ggggtttgta attttgagtc ttcataccag cacagcccgg ctgcaccatc
    22261 taacgccccg ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat
    22321 accatgcgaa tacggcaccg ccacacagaa gctggccccg gcaggcatac tgttccatac
    22381 cggcgtaaaa tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaagct
    22441 atgccagccg tcgttgctca tttcgaagcg tacgccagca aaagaaggca ttaatcgctg
    22501 gtagccaaac cccagcaacc tggcgctgac cacgatgcgc tggcgtactg gcgttttttc
    22561 agcagcacgc tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc
    22621 acttgcgatc gccgtggctt cggtatcctg ctggtcatct ttaaacagca tatccgtgct
    22681 ggaaaatacc aacatcttgt cctgataagt cgcgaacatc agggcgttat tgccgttata
    22741 gcgcaactga taaaccggga tggtttcact atttagctta atactactga tttccgtttt
    22801 gcttaactgg ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc
    22861 actgcgctgg atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc
    22921 cgcctgcgcg ggctgatcta agagtgaaga aaagagctta tctttcagcg tgagatcgtg
    22981 ttcatagaca atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata
    23041 atagaaaacg aaatcttcac tcagaacatc gtggagaaac ggaatggtaa ggagatcttt
    23101 gggaagctgg ctcagagagt cgctgtcgag aaagaggtcc ggctcattga gatcgatttg
    23161 cagattgttg tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg
    23221 caacgcccag acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa
    23281 ccgccagcct ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc
    23341 attttttacg agcacagcaa aactttaccg cgccctaata cgacaaaagc ccagactttg
    23401 cagcctggac ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa
    23461 attattcttc ttctggctcg tcgtcaacgt ccacttccgg agcgatttca tcgtcccctt
    23521 ccgcggcact gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgtt
    23581 gcagacccac tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac
    23641 ggcccacgat gctgatttcc gaaacgcgag tacgtaccag cgtaccggca tcggtgatca
    23701 tcatgatctg gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg
    23761 taaccttgat ggagataacc cctttcgtcg cacgcgactt agtcgggtat tccgccactg
    23821 cggtacgttt accataaccg ttctgcgttg cggtgaggat tgcgccatcg ccacgaggca
    23881 cgatcagaga gacgacttta tcgccttcgc ctaaacgaat accgcgaaca ccggtggtgt
    23941 tgcagcccat cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga
    24001 acagcattac ttcgtcttcg ccgctggtca ggtcaacgcc gatcagctca tcgccttcaa
    24061 ccagtttgat cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt
    24121 tcttcacagt accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca
    24181 ccggcaggat cgcggtgata cgttcgtcct gctccagcgg cagcaggttg acaatcggac
    24241 gaccgcgcgc gccacgagtg gcttccggca actgatagac tttcatcgaa tagacgcgac
    24301 cacggctgga gaagcacaga atatggtcgt gggtgttcgc caccagcagt cggtcaataa
    24361 agtcttcttc tttaatacgt gcggcagatt tgcctttccc gccacgacgc tgcgcttcgt
    24421 attcagaaag cggctgatac ttcacgtagc cctggtgaga gagcgtcaca accacatctt
    24481 cctgagtgat cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtgcgac
    24541 gtttgtcacc gaactgttca cgaaccagtt ccagctcttc gcggatcact tccatcagac
    24601 gatcggcact gccaagaata cgcagcagtt ctgcgatctg atccagcagc tctttgtatt
    24661 cgtcgagcag tttttcatgc tcaagaccgg tcagtttctg cagacgcaga tccagaattg
    24721 cctgggcttg ctgttcggtc agatagtaca gaccatcgcg cacgccgaac tctggctcca
    24781 gccattccgg acgcgcagca tcgtcgccag cacgttccag catcgcggca acgttgccca
    24841 gctgccacgg attagccacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga
    24901 tcagttcgat gatcgggtcg atgttcgcca gcgccacggc taatgcttca aggatatgag
    24961 cacgatcgcg agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt
    25021 gacgaacaaa cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca
    25081 atgccaccat gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttaa
    25141 gcacaacttc accaaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt
    25201 cagactcgtc acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa
    25261 tcttctcgat caggcgcgct ttgttcacct gatacggaat ttcatggacg ataatggttt
    25321 cacgtccggt tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc
    25381 gaccggtacg gtaagcttct tcaataccgc gacgaccatt aatgattgcc gccgtcggga
    25441 agtccggccc cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat
    25501 acgccagaca accgttgata acttccgtca ggttgtgcgg cgggatgttg gttgccatac
    25561 ctacggcgat accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt
    25621 cgggaatttt ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat
    25681 cggccatcag ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg
    25741 cagagtcgcc gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg
    25801 agaatggctg cgccatacgg acgatcgtgt tataaaccgc caagtcacca tggggatggt
    25861 atttaccgat tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat
    25921 tgcctagtac gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga
    25981 catctggcag cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttca
    26041 gctcttcctc aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat
    26101 ccctctactg tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg
    26161 taaacctata cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac
    26221 ggagtagaag tgccaatgaa tgccgaaaaa tcgccggtaa accataacgt agaccacgaa
    26281 gagatcgcta aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa
    26341 ccgctgcacc gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta
    26401 tttggcaaaa aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg
    26461 cgcgaaggtg cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag
    26521 ctgcacgcgc tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac
    26581 gcggcaaaac atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgtg
    26641 cccgatccgc agtcagtggt cagagcctgt gcgcaactgg tgaaaccggg cggcgatgtc
    26701 tttttctcga cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa
    26761 tatattttgc gcatggtgcc caaaggcacg cacgacgtga agaaatttat aaaaccggca
    26821 gaactgcttg gctgggtaga tcagacaagc cttaaagagc gacatataac cgggttacat
    26881 tacaacccga tcactaatac ttttaaactc ggccccggcg tcgatgtgaa ctatatgctg
    26941 cacacgcaga ataaataaaa ttgttagccg gataaggtgc tcgcaccaca tccggctaca
    27001 atgccgggcg caaagcagct accatttcac cgtcatcgac aaaaaccctg ccgtctgggc
    27061 aaaatcatca ctccctttct gccacgccac gctaccacgc agggacactc gctgactgat
    27121 attgcccgtg actcccactt ttatttcacc ccgttgtttt accgcttcgt cactgatagt
    27181 gctgccatct tcttcaattt tcgtcgaatg gggatcgtaa taataattca gatctaatgt
    27241 tggtgtgaca tgaacagcgg tacgccattc gctgtgtaaa cccagccgcg tctgaatatc
    27301 atcgccctgt gattgtgaca cgcgcgcatg gttagcggct gtaaaatcat cctgctgcac
    27361 gccctgataa atcacctgcg cctgtggttc aatcaccact ccatgccccg gtaaccactg
    27421 atacccggct tccagcgagg cgataatccc cgatgagtgg taatgatcca cgccatcttc
    27481 atgttcggaa acatcattgt taaaccacgc gtattgcagc cagctatcca gccaggcccc
    27541 ctgcttctga ttaccgtgct gaaaccagct tgaggtcaga ccaacggcat aaccgtggtt
    27601 ctggttatcg gcgcgagttc cggtcatatt cgagcggctg tcgccctggt tatcactgta
    27661 gccaccaacc gccccaagca tccactcgcc atccgtgccc cagcgcccgc taaacaagtc
    27721 gccgctaagc tgcaccgtag aagtgtcttc atgctgagcc agttgccccg ctgctgtgta
    27781 atgataatct ccgccgataa cacgtaaatt cagcgtctga ccattgccac cagcgtgatc
    27841 gtgtcgctcc atcacaaacg cctgatttgc cgcccgcagg ttattaaaat atccaccaac
    27901 tttggcattc aacaccggct gataagcagg cgtagggtcc ggggccgggt cgggtgttgg
    27961 atcaggatcc ggcgtgggat caggatcggg agtcgggtct ggatcaggtg gcgatggtgg
    28021 cgttacttct tgcgatcgca gataccagtc gttgttatct tccaccagcg tgtagtcata
    28081 cgctcccata ttgacgtagc cgctgcccgc cagactgaac tgcgcattgt tttgaaattg
    28141 cgttggatcg gctgcgaaat caaccacttt aatgcctgtc gatgtcggct caccaatacc
    28201 cgtaatggga ttaattacca cggtcgtgtt gccagcagta ttaccgttca acaccagttg
    28261 gtcacttgcc gagtcatcgc cgtttaattc gctatcgagc agtagcgtac caccgccagt
    28321 ataatcaccg tttaccctca gcgtgtcgcc agcgacgcca ttttgcaggc tgaccgcacc
    28381 ggacgtattg gtcagattgc cattaactgt tacgtcggaa agataagtca ggctgtcagt
    28441 ttcaaggttg gtattaagct cacctgtcaa cgtcacgcca tcggtggtat tcaccagcgt
    28501 cccatcgccg ttaaacagtg aggcattaag ggcgacggaa gtatcctgcc cggtcaggcg
    28561 caaaaccgta ccatcgctga tgtcgatagt gccgctggaa gtagcatcaa ttgactgaat
    28621 atcctgatcc gcgccagtaa cgaacgttgc cccgtcaccg acccacagcg acgaagcata
    28681 agggaagata tccgcaacat cgccttgcag cgtgccttct tcgacacgta ccgccgactg
    28741 cgtcgtaccg ctggcggtca gttccagcgt acccgccccc gttttcgtca atgtgctacc
    28801 ctcatcctga tgctgcccgc tgctgtcagc catcagtgcg ccccactgcg tgtctacccc
    28861 cgcatcaact gccacttcac cgtcggcgcg catttcaata tcacgcccat gctcagtcgt
    28921 tgtagagcga gtatcgacat tggcagtaat ctccatcacg ctttcttgtt gattatcggt
    28981 aaagataacc tggcggttgt aatgggtatc tccaagctgc gagttgtcgc tcaccaccag
    29041 cgtaccggaa gcgatttgcg tggtgcccag gtaactatta ttgtttgcca ggctaatcag
    29101 cccgtcgccc gcatcgacca ccacgtagac gccatctttc ccgtcgccgc cgatatgcat
    29161 attgtttccg cctgcaagat ctaccacatc ctgactgccg ataaccgttc cgctgctgcc
    29221 gctgactgca aggccatcgt tgtatgatgt gccgctctgc caggtggcga aatcagagag
    29281 atcaagcaca ccgccgttta acacgatcga ctgcggatcg tcctggagag cggcaagatc
    29341 tgccgcgtcg ccttccagcg taagcaccgc gccatcatca acgactatat cgccagttag
    29401 cgccatcgac tgtgcccctg ccagcacgta gctgccgttt tgcgcaatgg tgagctgacc
    29461 agcaccttcg atggtgccag caaaactgcc ttgattaaca gtaacattgc caccagcatc
    29521 gatatttaaa gtgccattct gaaagcccgt caatgcgtgc acaaaagttt gttgggtcga
    29581 gccaacgttt agctcagcct gattctgata ctgatcaata ctccctatcg tcagaccgta
    29641 gcagtcttgc ggatcgtcct ggcaatgcgt atcgccgaca ttcatcaggg agttgctgcg
    29701 gcccagggta acttcaccgt tttctatctg catctcgcca gtaaagtcat tgttatttgc
    29761 attaagtacc agatcgccgg aacctgtttt ggtgattacc ccggtaccag caatagagtc
    29821 aacagctccg tcattctctg tattgccgat aaccagcgtt tttccgtcgg caatatcaaa
    29881 ggtaacttca cttaagccga gatacataaa gccgcccgcc gcagtggaag gaccatcccc
    29941 ataacctgct gcgctgttgt tctcatccac taacacgccg ttgttctggc tgtagctgtc
    30001 atcaactgaa atatcaataa gatagggagc cgtcacgcta ttggtataaa tcgccccacc
    30061 atatccctca gcagagttat ttgtaaatgc ggtgttattt attattgtga aacctgacgg
    30121 atgcgtgata ttgctattat tatcggtgac atcgattgcc cctccatcgc catcactgta
    30181 acttgttgat gtatatgcct ggttgttatc aaaaataact tcacttaaat agacgtcatt
    30241 gttgatggta taaattgcac cacctttgcc gtcattagcg atgttattgc gaaacacggc
    30301 gttagtgaca cgtaaatcga cgacaccggt atcgttagta ccagaagaat agattgcgcc
    30361 accatagcca cctgcaacgt tatcggaaaa aataacatca gtaagattca gcgttgagtt
    30421 ttctttagca aatattgccc cgccgttatt atattctcct gagacagtat tattggcaaa
    30481 cagagtcatt ccagtttcat tttctggcga tagagaaaac tccgctcctt gttgcaaaaa
    30541 tacggcccca ccgctggcgt tattgttcat atccgaaaaa accagccatt gcccatcggc
    30601 gatgctccag tcctgcgtaa tgcctgaaag gctttgcctg ctggcgtgac aactcgcttt
    30661 gatatcatat ccctgacatg aatcaatgac cgccgcgaca ccgttagcag agaaaagaga
    30721 tgcaatcatt gacgggagta aagataaata ctccttgcgt agaaaaataa tccgcatatt
    30781 aatctgacca tcattttcta taagacggca tatttaatcg cattatacga tatgggttat
    30841 ttttcttttg taaattgttc aacccggtgc aatggctatt ttgttattat ttcatgaata
    30901 atatcagtgc gtcataattc aagttaatca ccttaaggga tatcagttat gtttaaactg
    30961 aattaaagta atgaataatt ttcttataat ataaggtaaa ttaacaaaat ggcttcgcat
    31021 ttaacaacaa ccgaagagaa aacaaccatt tcgacatcaa caatcgctta cattcgctta
    31081 tatattgacc acaactgata catcagatta tgtgatgact cgtacttaga tcaatttttg
    31141 caatcattag caaaaagatt aataaaccat ctatatcaat ttatccaacc tattctgccg
    31201 ttcaagaaat cgccgaacag ttatttttaa caaatttttc tcttcccatt gactttcccg
    31261 gacaccttgt ctgacctaag gtgcgcgaaa gccacttttt ccttcctgag ttatccacaa
    31321 agttatgcac ttgcaagagg gccattttca cactatcttg cagtgaatcc caaacatacc
    31381 ccctatatat agtgttctaa gcagcttccc gtactacagg tagtctgcac gaaactattg
    31441 cggaaagaat tccaaaaaca ggtacgacat acatgaatca gaatctgctg gtgacaaagc
    31501 gcgacggtag cacagagcgc atcaatctcg acaaaatcca tcgcgttctg gattgggcgg
    31561 cagaaggact gcataacgtt tcgatttccc aggtcgaact gcgctcccac attcagtttt
    31621 atgacggtat caagacctct gacatccacg aaaccattat caaggctgcc gcagacctga
    31681 tctcccgtga tgcgccggat tatcagtatc tcgccgcgcg cctggcaatc ttccacctgc
    31741 gtaaaaaagc ctacggccag tttgagccgc ctgcgctgta cgaccacgtg gtgaaaatgg
    31801 tcgagatggg caaatacgat aatcatctgc tggaagacta cacggaagaa gagttcaagc
    31861 agatggacac gtttatcgat cacgaccgtg atatgacctt ctcttatgct gccgttaagc
    31921 agctggaagg caaatatctg gtgcagaacc gtgtgaccgg cgaaatctac gaaagcgccc
    31981 agttccttta tattctggtt gccgcgtgcc tgttctcgaa ctacccgcgt gaaacgcgcc
    32041 tacaatacgt gaagcgtttt tacgacgcgg tttccacatt taaaatttcg ctgccgacgc
    32101 caatcatgtc cggcgtgcgt accccgactc gtcagttcag ctcctgcgta ctgatcgagt
    32161 gcggtgacag cctggattcc attaacgcca cctccagcgc gattgttaaa tacgtttccc
    32221 agcgtgccgg gatcggcatc aatgccgggc gtattcgtgc gctgggtagc ccgattcgcg
    32281 gtggcgaagc gttccatacc ggttgtattc cgttctataa gcatttccag acagcggtga
    32341 aatcctgctc tcagggcggt gtgcgtggcg gtgcggctac gctgttctac ccgatgtggc
    32401 atctggaagt ggaaagcctg ctggtgttga aaaacaaccg tggggtggaa ggcaaccgcg
    32461 tgcgtcatat ggactacggg gtacaaatca acaaactgat gtatacccgt ctgctgaaag
    32521 gtgaagatat cactctgttc agcccatccg acgtaccggg gttgtacgac gcgttcttcg
    32581 ccgatcagga agagtttgaa cgcctgtaca ccaaatatga gaaagacgac agcatccgca
    32641 agcagcgtgt gaaagccgtt gagctgttct cgctgatgat gcaggaacgt gcgtctaccg
    32701 gtcgtatcta tattcagaac gttgaccact gcaataccca tagcccgttt gatccggcca
    32761 tcgcgccagt gcgccagtct aacctgtgcc tggaaatcgc cctgccgacc aaaccgctga
    32821 atgatgtcaa cgacgagaac ggtgaaatcg cgctgtgtac gctgtctgct ttcaacctgg
    32881 gcgcaattaa tagcctggat gaactggaag agctggcgat tctggcggta cgcgcactcg
    32941 acgcgctgct ggactatcag gattacccga tcccggccgc caaacgtgga gcgatgggtc
    33001 gtcgtacgct gggtattggt gtgatcaact tcgcttacta cctggcgaag cacggtaaac
    33061 gctactccga cggcagcgcc aacaacctga cgcataaaac cttcgaagcc attcagtatt
    33121 acctgctgaa agcctctaat gagctggcga aagagcaagg cgcgtgcccg tggtttaacg
    33181 aaaccactta cgcgaaaggg atcctgccga tcgataccta taagaaagat ctggatgcca
    33241 tcgctaatga gccgctgcat tacgactggg aagcactgcg tgagtcaatc aaaacgcacg
    33301 gtctacgtaa ctccacgctt tctgctctga tgccgtccga gacctcttcg cagatctcta
    33361 acgccactaa cggtattgaa ccgccgcgtg gttacgtcag catcaaagcg tcgaaagacg
    33421 gtattctgcg ccaggtggtg ccggactacg agcatctgca cgacgcctat gaactgctgt
    33481 gggaaatgcc gggtaacgat ggctatctgc aactggtggg tatcatgcag aaatttatcg
    33541 atcagtcgat ctctgctaac accaactacg atccgtcacg cttcccgtca ggaaaagtgc
    33601 cgatgcagca actgctgaaa gacctgctca ccgcctacaa attcggggtc aaaacgctgt
    33661 attatcagaa cacccgtgac ggcgctgaag acgcgcagga cgatctggta ccgtcaatcc
    33721 aggacgatgg ctgcgaaagc ggcgcatgta agatctgata ttgagatgcc ggatgcggcg
    33781 tgaacgcctt atccggccta cggctcggtt tgtaggcctg ataagacgcg ccagcgtcgc
    33841 atcaggcaca ggatgcggcg taaaatgcct tatccggcat taaactccca acaggacaca
    33901 ttcatggcat ataccacctt ttcacagacg aaaaatgatc agctgaaaga accgatgttc
    33961 tttggtcagc cggtcaacgt ggctcgctac gatcagcaaa aatatgacat cttcgaaaag
    34021 ctgatcgaaa agcagctctc tttcttctgg cgtccggaag aagttgacgt ttctcgtgac
    34081 cgtatcgatt accaggcact gcctgaacac gaaaaacaca tctttatcag caacctgaaa
    34141 tatcagacgc tgctggactc cattcagggt cgcagcccga acgtggcact gctgccgctg
    34201 atctcgatcc ctgaactgga aacctgggtc gaaacctggg cgttctcaga aaccattcac
    34261 tctcgttctt atacccatat cattcgtaat atcgttaacg atccgtccgt tgtgtttgat
    34321 gatatcgtca ccaacgagca gatccagaaa cgtgcggaag ggatctccag ttattacgat
    34381 gagctgatcg aaatgaccag ctactggcat ctgctgggcg aaggtaccca caccgttaac
    34441 ggtaaaaccg taaccgttag cctgcgcgag ctgaagaaaa aactgtatct ctgcctgatg
    34501 agcgttaacg cgctggaagc gatccgcttc tacgtcagct ttgcttgttc ctttgccttt
    34561 gcagaacgcg aactgatgga aggtaacgcc aaaattattc gcctgattgc ccgcgatgaa
    34621 gccctgcacc tgaccggtac tcagcatatg ctgaatctgc tgcgcagcgg cgcggatgat
    34681 ccggagatgg cggaaattgc cgaagaatgt aagcaggaat gctatgacct gtttgtccag
    34741 gcggcacagc aagagaaaga ctgggcggat tatctgttcc gtgacggttc gatgattggt
    34801 ctgaataaag acattctctg ccagtacgtt gaatacatca ccaatatccg catgcaggca
    34861 gtcggtttgg atctgccgtt ccagactcgc tccaacccga tcccgtggat caatacctgg
    34921 ctggtgtctg ataacgtgca ggttgctccg caggaagtgg aagtcagttc ttatctggtc
    34981 gggcagattg actcggaagt ggacaccgac gatttgagta acttccagct ctgatggctc
    35041 gcattaccct gcgcatcact ggcacacaac tgctgtacca ggatgaacac ccttcccttc
    35101 tggcggcgct ggaatcccac aatgtggcgg ttgagtacca gtgccgcgaa ggttactgcg
    35161 gttcctgtcg cacgcgcctt gttgcaggcc aggtggactg gattgccgaa ccgttagcct
    35221 ttattcagcc gggggaaatt ttgccttgtt gttgccgggc aaaaggcgat attgaaatcg
    35281 agttgtgatc gggtactagc gccagatacg acgcttacgc gtattatctg gcctacaacg
    35341 attacatggc gtagtaatac gctttcacct gctcccagtc cgctttgggg atcggctcca
    35401 gatatttttc cagctggcgg aagtcatgat taatcgcttt atcgcgacgc aagcggcgac
    35461 gacttttctc cagatcaaga aatccagctt ctgccttacc ttctgttttc acatagatat
    35521 ggcgaacata gcaacagcca tgctgacgat taacgctatg cattttctta aacgccagcg
    35581 ccactgcttt caacatggct tgccgtactt cgtcagaata gggcgttact gcatgccggg
    35641 cataccagtc agcaatgctg atgaaccccg ccatatcttc agtcaccagc aacgcgcgcc
    35701 attccccctc aattttcacc gcttcaccaa aaacaatctt cggcacgatg acaccagccc
    35761 gctccagttc tttaattacc gcaacctcac ggacaatcgt tggtcggccg aacggataac
    35821 gtacggaatg aaacagatga tgcgtcatgc gctttacata tagctttttg ccgttgcgct
    35881 cgacgcattg caccccgctc ataccattac ggcgatagtt aggctcttca acccagtcgc
    35941 cctctgttgc ccaccagtga ttaaattcgt cgtgctttgc tgaaactgcc ataccctatc
    36001 gcctgtcatt ttttatttac gacaatgact ataggtggtt acctgcgaaa aatcttaatg
    36061 aaacgtgtcg tattaatgaa tttcttttgc cactactagc gcgacaccgc ttttaccctt
    36121 catttgcagc gcatagactc cgccagccac taccactaat agtgcaccca gcagataaat
    36181 cattcggtag ccagtatgtg caaccatcat ccccatcaca caggctccca gcgccaggcc
    36241 tgcatccatc gcattgaaga agagggagtt tgcgacaccg attttatgcg gttccactga
    36301 actgataatc tgcgtctgaa aaactggcgt cacggaacca tacccgatac caatcaatgc
    36361 gccagaaaca accatcatta tgcttccctg ggtgtagccg agaaccacca gtcctaccgt
    36421 aaagaacaca aggcaagggt aaaccacata tttcggtcct tttttgtcgc agacattgcc
    36481 ggtaaaggta cggcaaatca tcaggaagat agcgtagcaa agcaggaaat tactggctgc
    36541 cgacatcagg ttaagttcgc gtgcataaag cgccagaaaa gcagaaaccc cggcatacga
    36601 aaaggtcata aaaaaggtga ccatcgcaaa tggcaacgcc gcgcgatcga acatggcggc
    36661 gaagcccatt ttcggcgttg aaccatcagc atggctgata accggcggca ctgtcataat
    36721 cagcgacaga ataatcccca ccgctgccac gccggtgcat agccagaaag ccagattaaa
    36781 gccttcccag cgcgccatat tcagaccaat ccacggcccg accaccattg ccagccccat
    36841 tgccagagag aaaaaactga tgccctcccc acgtcgggag gccgggatca gccgcgcgga
    36901 aatcgttcct tttaccgtag tgataacccc gaaggtaata ccatgaagca cccgaataaa
    36961 aagcagtgat tcaattgagt ggcaaaaagg atacagcgca gtgaccacca aaaaggccag
    37021 agaagagagc cccagaatag ttttattcga atatttacct acccattgcc cggcaaaggg
    37081 gcgaataaca atggcggcaa ttaaaaataa agtcaccaac aaacctgctt tatcaggaga
    37141 ggcatgcaag cggtctgaga tataaattgg caacaatgtt agtaaaacat aaaagacgaa
    37201 gaaaataata aagctgatga tggttattgc ccaaaaatcc ttcgtccata acttctcttt
    37261 cataccctac cgcctgtcag gatgacgcac gcagacaaca ctcccgttac tgcggttgcg
    37321 tcaaattgtg atgtactttg cagaacagaa gtataaatgg cggcacgcta ttagagaaat
    37381 taatcgtttt aatattattg attagcaaac tctaatgact cttactcaaa taaattcttt
    37441 actcgccgtg ctggattacg gtggctttac tgaagccagc aagcgattgt tcatgacgca
    37501 gtcggcggta agtcaggcaa ttgctgccct cgaacaagaa cttggcgtga gtatactgtt
    37561 acgcgatcgc cgtaaagcta tacaactgac cgcagcaggt catcgcattg tgcatcatct
    37621 gcgcgccatt aatcgggaag tgaacgcagt aaaagagata gccgagcagg aaaagcagaa
    37681 ccctcaaaga acgctgagac ttggttgttt ccccagcgtc tgcgcctgta tcttaccggc
    37741 tgtgattcgc tactttgaaa ttcatcatcc gaatataaaa attattcctt ttgaggaaaa
    37801 tagcacagcg attattgatt cccttcagaa tgaatccatt gatgccggtt ttgttcattt
    37861 tccggtgagc ggaatgtact ccgtgcccat ttatcaggat aaattcaccg tagtgttacc
    37921 gcccaaccat gctctggcaa aaaacagcac tattacagtg gaagagttaa tgggtgaacc
    37981 attaattatt agtaaaggac gctatgaatt aagcattatg gcactattta aagaaaagaa
    38041 tattacccca cagataaaat atgaatttaa tcacccggat acagcgatca gttttattcg
    38101 acagggatta ggcatcgcct tattaccaga gttaacgctg aaaaccattg cagatgaatt
    38161 gtgttccgtt cctctcgaac caaacttcta tcgacagatt tcgttgctgg ctaaagaaaa
    38221 gccggtagaa ggcagtccac tgtttttact gcaaatgtgc acggaacaat tagtggagat
    38281 tggaaaaatt tgatgtgaga caggatggcg catgaacgcc atcctgcaat agcattactc
    38341 tttattaagg aattttactg ccttatcagg gaaatcagta aacagcccat ttacacccgc
    38401 tttgttatac agagcatcat ataactgatt cacatcagta gtgtattcag gcagcttatc
    38461 tgaccgcacg gtgtaaggat gcactaccag tttattctgc tgagcatctt gcaccatgcc
    38521 agtgagtttg atattacctg gctgcgatgt ctcctcaatc aacatatgat aatccggacc
    38581 aataccatca gcatattccg ccacctgttt catggccccc ggcttaaaca tccagtcgta
    38641 gttgtaatta acccagcttc cgtccggctg tttctgctga gtttcgttcc agtcggtata
    38701 ggcaatcagc tgtaccagat tgaggtccat gcccatttta ggttccagct cattcttaat
    38761 acgcttcagt tcatcggcat caaaacattg caaataaact ttgtcgtcct taccgctgta
    38821 accatatttc ttcagcactt ccagcgtttt agccgcaata tccttccctt cctgatgatg
    38881 gaaccacggc gctttaattt ctggatagat accgatattt ttcccggtag agtgatttaa
    38941 cccctgaaca aattcaatct cttcttcgaa agtgtgtacc cggaagtcag atttacccat
    39001 cgggaaacgc cccggataag tctgtacttt cttaccgttt tcaatatcga aaccttcggt
    39061 aaatttcagc gacttaattt catcgagcgt gaaatctatc gcgtagtaac gaccgtcttt
    39121 gcgcgcccga tccgggaaac gatcggcaac atcagtaaca cgatcgagat aatggtcatg
    39181 caggacaacc agatggtcgt ccttcgtcat caccaaatcc tgttccagat aatccgctcc
    39241 ctgcgcatac gccatcgctt ttgctggcag cgtatgctcc ggcaaatatc cactggcacc
    39301 gcgatgggcg atgactattt tttcgttgct atccgccgcc attgcactgc ttcccatgat
    39361 cattccactc atcatgatcg ccatgctaag gttttttagc gtcaatttca tgccattagc
    39421 ctccgttgcg tttttgcagt aattcctgat ggcgacgttt ttcgccaatc atcacaacaa
    39481 tcaacaagat aaccgcgaga atgctgccgc caatcattac cataaagccg ccatcccagc
    39541 cgaagaagtc cacggtgtag ccaacaatcg cgctcgccgc caccgaaccg cccaggtaac
    39601 caaacagtcc ggtaaagccc gctgccgtac ctgccgcttt tttcggtgcc agttccagcg
    39661 catgcagacc gatcagcatc acagggccgt agatcaggaa gccgataaca atcatacaaa
    39721 tcatatctac ggttgggtta cctgccgggt tcatccagta aacgatagtc gcgatggtca
    39781 ccagtgtcat aaagaagacg ccggtggccc cacggttgcc acggaagact ttatccgaca
    39841 tccagccgca cagcagggtg cccggaatgc ccgcatactc atagaagaag taggcccagg
    39901 aggatttatc cagcgcgaag tgtttcacct ctttcagata agtcggtgac cagtcgagga
    39961 tgccgtaacg cagcagataa acgaacacgt tggcgatagc gatgtaccac agcagtttgt
    40021 tcggcagcac gtactgcatg aagatttgct tcgccgtcag ctcctgttcc gctttttcgt
    40081 tatagtcgtc cggataatca tttttgtact cttcgatcgg cggcaggcca caagattgcg
    40141 gggtatcgcg catcatcgca aaggcgaaca gtgccaccag aatggcgcag aaagcgggca
    40201 tatagagcgc cgcgtgccag tcattgaacc aggccatccc cagcaggaac agcagcggcg
    40261 gaataccacc accgacgttg tgtgcacagt tccacactga cacaatgccg ccacgttctt
    40321 tctgcgacca ccagtgcacc atagtacgac cacacggcgg ccaccccatc ccctggaacc
    40381 aaccgcagag gaacaacagt acaaacatca ccgcgatgct cgacgtcgcc cacggcacaa
    40441 agcccataaa caacatcact gccgccgcca gaatcaaacc tgcgggcagg aaaacgcgcg
    40501 gattcgagcg atctgacacc gagcccatga tgaatttcga aaatccataa gcaatcgaga
    40561 tccccgaaag ggcaaaacct aaatcaccgc gtgagaagcc ctgctcgacc agataaggca
    40621 tagcgagggc aaagttctta cgaaccaaat agtaagcagc ataaccaaag aatatcccca
    40681 ggaaaatttg ccagcgcaat cgacgataag tcggatcgat ctccgcggca ggtaagcgcg
    40741 ctttgtgtgg cgctggttta aaaatactca acattgatag cctccgtggc ccgtggtctt
    40801 atttatgatt aacagcctga ttcagtggga gaacctgccg tttcttgagt tgccgcgatg
    40861 ttaagaaaac attcataaat taaatgtgaa ttaccgcaca cattattaaa tacgatgggt
    40921 taaatgttca taatgacgca tgaaatcacg tttcactttc gaattatgag cgaatatgcg
    40981 cgaaatcaaa caattcatgt ttttactatg gctaaatggt aaaaaacgaa cttcagaggg
    41041 ataacaatga aaactcgcga ctcgcaatca agtgacgtga ttatcattgg cggcggtgca
    41101 acgggagccg gaattgcccg cgactgtgcc ctgcgcgggc tgcgcgtgat tttggttgag
    41161 cgccacgaca tcgcaaccgg tgccaccggg cgtaaccacg gcctgctgca cagcggtgcg
    41221 cgctatgcgg taaccgatgc ggaatcggcc cgcgaatgta ttagtgaaaa ccagatcctg
    41281 aaacgcattg cacgtcactg cgttgaaccg accaacggcc tgtttatcac cctgccggaa
    41341 gatgacctct ccttccaggc cacttttatc cgcgcctgcg aagaagcagg gatcagcgca
    41401 gaagctatag acccgcagca agcgcgcatt atcgaacctg ccgttaaccc ggcactgatt
    41461 ggcgcggtga aagttccgga tggcaccgtt gatccatttc gtctgaccgc agcaaatatg
    41521 cttgatgcca aagaacacgg tgcggttatc cttactgctc atgaagtcac gggactcatt
    41581 cgtgaaggcg cgacggtgtg cggcgttcat gtacgtaacc acctcaccgg cgaaacccag
    41641 gcacttcatg cgcctgtcgt ggttaatgcc gccgggatct gggggcaaca tattgcggaa
    41701 tatgccgatc tgcgcattcg catgttcccg gcgaaaggat cgctgctgat catggatcac
    41761 cgcattaatc agcatgtgat caaccgctgc cgcaaacctt ccgacgccga tattctggtg
    41821 cctggcgata ccatttcgct gattggcacc acctctttac gtattgatta cagcgagatt
    41881 gacgataatc gagtgacggc agaagaagtt gatattctgc tgcgtgaagg ggaaaaactg
    41941 gcccccgtga tggcgaaaac gcgtattttg cgggcttact ctggcgtgcg tccgctggtt
    42001 gccagcgatg acgatccaag cggacgtaac gtcagccgtg gcatcgtgct gctcgatcat
    42061 gctgaacgcg atggtctgga cgggtttatc accatcaccg gtggcaaact gatgacctac
    42121 cgcctgatgg cggaatgggc tactgacgcg gtgtgtcgca agctgggcaa cacgcgcccc
    42181 tgtaccactg ccgacacgcc gttgcctggc tcacaggaac cggcagaagt cacgctgcgc
    42241 aaagtgatct ccctgcccgc tccgctgcgc ggttccgcag tttaccggca cggcgatcgc
    42301 acaccagcct ggctgagcga aggccgcctg caccgcagcc tggtatgtga gtgcgaagcg
    42361 gtaactgcgg gtgaagtgca gtacgcagta gaaaatttaa acgttaacag cctgctggat
    42421 ttacgccgtc gtacccgtgt agggatgggt acctgccagg gcgaactctg cgcctgccgg
    42481 gctgccggac tgctgcaacg gtttaacgtc acgacgtccg cgcaatctat cgagcaactt
    42541 tccaccttcc ttaacgaacg ctggaaaggc gtgcaaccca tagcatgggg agacgcactg
    42601 cgcgaaagcg aatttacccg ctgggtttat cagggactgt gtggtctgga gaaggagcag
    42661 aaagatgcgc tttgatactg tcattatggg cggcggcctc gccggattac tctgtggcct
    42721 gcaactgcaa aaacacggcc tgcgctgtgc cattgtcact cgtggtcaaa gcgcgctgca
    42781 tttctcatcc ggttcgctgg atttgctgag ccatctgccg gatggtcaac cggtgacaga
    42841 cattcacagt ggactggaat ctttgcgtca gcaggcacct gcccatcctt acactcttct
    42901 cgggccacaa cgcgtactcg atctcgcttg ccaggcgcag gcattaatcg ctgaaagcgg
    42961 tgcacaattg cagggcagcg tagaactggc tcaccagcgg gttacgccac tcggcactct
    43021 gcgctcaacc tggctaagtg cgccagaagt ccccgtctgg ccgctgcccg cgaagaaaat
    43081 atgtgtagta ggaattagcg gtctgatgga ttttcaggcg catcttgcgg cggcttcgtt
    43141 gcgcgaactc gaccttgccg ttgaaaccgc agaaattgaa ctgccggaac tggatgtgct
    43201 gcgcaacaac gccaccgagt ttcgcgcggt gaatatcgcc cgtttccttg ataatgaaga
    43261 aaactggccg ctgttacttg atgcgcttat tcctgtcgcc aatacctgcg aaatgatcct
    43321 gatgcccgcc tgcttcggcc tggccgatga caaactgtgg cgttggttga atgaaaaact
    43381 accttgttca ctgatgcttt tgccaacgct gccgccttcc gtgctgggca ttcgtctgca
    43441 aaaccagtta cagcgccagt ttgtgcgcca gggtggcgtg tggatgccag gcgatgaagt
    43501 gaaaaaagtg acctgtaaaa atggcgtagt gaatgaaatc tggacgcgca atcacaccga
    43561 tatcccgctg cgcccacgat ttgcggtgct tgcgagcggc agtttcttta gcggcggact
    43621 ggtggcagaa cgtgacggca ttcgcgagcc gattctcggc cttgatgtgc tacaaacctc
    43681 cacgcggggt gaatggtata agggcgattt ttttgcaccg caaccgtggc agcagttcgg
    43741 tgtaaccact gatgaggcac tacgcccgtc gcaggcagag caaaccattg agaacctgtt
    43801 tgccatcggt tcggtgctgg gcggatttga tcccatcgcc cagggatgcg gcggcggtgt
    43861 ttgtgccgtc agtgctttac atgccgctca acagattgcc caacgcgcag gaggtcaaca
    43921 atgaacgaca ccagcttcga aaactgcatt aagtgcaccg tctgcaccac cgcctgcccg
    43981 gtgagccggg tgaatccggg atatccgggg ccaaaacaag ccgggccgga tggcgagcgt
    44041 ctgcgtttga aagatggcgc actgtatgac gaggcactga aatattgcat caactgcaaa
    44101 cgttgtgaag tcgcctgccc gtccgatgtg aagattggcg atatcatcca gcgcgcgcgc
    44161 gcgaaatatg acaccacacg cccgtcgctg cgcaattttg tgttgagtca taccgacctg
    44221 atgggtagcg tttccacgcc gttcgcacca atcgtcaata ccgctacctc gctgaaacca
    44281 gtgcggcagc tgcttgatgc ggcgttaaaa atcgatcatc gccgcacgct accgaaatac
    44341 tccttcggca cgttccgtcg ctggtatcgc agcgtggcag cccagcaagc gcagtataaa
    44401 gaccaggtgg ctttctttca cggctgcttc gttaactaca accatccgca gttaggcaaa
    44461 gatttaatta aagtgctcaa cgcaatgggt accggtgtac aactgctcag caaagaaaaa
    44521 tgctgcggcg taccgctgat cgccaacggc tttaccgata aagcacgtaa acaggcaatc
    44581 acaaatgtag agtcgatccg cgaagcagtg ggagtaaaag gcattccggt gattgccacc
    44641 tcctcaacct gtacatttgc cctgcgcgac gaatacccag aagtgctgaa tgtcgacaac
    44701 aaaggcttgc gcgatcacat cgaactggcg acccgctggc tgtggcgcaa gctggacgaa
    44761 ggcaaaacgt taccgctgaa accgctgccg ctgaaagtgg tttatcacac tccgtgccat
    44821 atggaaaaaa tgggctggac gctctacacc ctagagctgt tgcgtaaaat cccgggactt
    44881 gagttaacgg tgctggattc ccagtgctgc ggtattgcag gtacttacgg tttcaaaaaa
    44941 gagaactacc ccacctcaca agccatcggc gcaccactgt tccgtcagat agaagaaagc
    45001 ggcgcagatc tggtggttac cgactgcgaa acctgcaaat ggcagattga gatgtccaca
    45061 agtcttcgct gcgaacatcc gattacgctg ctggcccagg cgctggctta aactcctttc
    45121 tgatgcccgg taagcatgtt gttaccgggc atttttgcgt acacgattcc gtgcccaatg
    45181 tatgcgttgc aacgcagtga aaattcctct gaaaacgtct cgcaaaggct aaaactggca
    45241 gatgtcaaag gcctgggata accgtaatgt cgcgtcatca taaatatcag gtgacggaca
    45301 accatgaccg aatcaacaac ctcctccccg catgatgcgg tgtttaaaac ctttatgttc
    45361 acacccgaaa ccgcacggga ttttctggaa atacatctac cagagccgct gcgcaagctt
    45421 tgcaacctgc aaaccttacg cctggaaccc accagtttta ttgaaaaaag tttacgcgct
    45481 tactactcgg atgttttgtg gtccgtggaa accagcgacg gtgacggtta tatctactgc
    45541 gtgattgaac atcaaagctc tgcagaaaag aatatggctt ttcggctaat gcgctatgcc
    45601 actgccgcca tgcagcgtca cctggataaa ggctatgaca gagttccgct ggtggtgcca
    45661 ttgctgtttt atcatggcga aacctcgccc tacccgtact cactcaactg gctggatgag
    45721 tttgacgatc cgcaacttgc ccggcagttg tacaccgaag cttttccgtt ggtggatatc
    45781 accatcgtac ctgacgatga gattatgcaa catcggcgta tagctctgct tgaactgatt
    45841 caaaagcata ttcgcgaccg cgatttaatc ggtatggtcg acaggatcac cacgcttttg
    45901 gttagaggct tcactaatga cagccagcta caaacgctgt ttaattatct gctgcaatgc
    45961 ggcgatacct cccgtttcac ccgttttatt gaggagattg ccgaacgttc accactacaa
    46021 aaggagagat taatgactat tgctgaacgg ctacggcagg aaggacatca aattggctgg
    46081 caggaaggta aaattgaagg ttggcaggag gggaaattgg agggtttgca gaaaggcaaa
    46141 gtagaaggta tgcatgaaca agccattaaa attgccttgc gcatgctgga acagggcttt
    46201 gaacgtgaga ttgtgctggc gacaacccaa ctcactgatg ctgatattcc gaactgttat
    46261 taaaaggaga gattaatgac tattgctgaa cggctacggc aggaaggaca ccaaattggc
    46321 tggcaggaag gtatgcgtaa acaagccatt aaaattgctt tgcgcatgct ggtacagggc
    46381 attgatcgtg acctggtgct cgcagccacc cagctaagcg aagccgatct ggcagcgaat
    46441 aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat caataattac
    46501 cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga tccagggcat
    46561 cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac gccaggcatt
    46621 gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca gcacggatcc
    46681 gccgaatact ggtttcaata attcgctgca cttccgggtg cccggcgtta tccgggtagc
    46741 ccaacgaggc agaaagatcc gcaggtccaa taaacacgcc gtcaatccct tcgacgtcga
    46801 ggatttcgtc caggttatcc agtgctgttt tactttccac ctgcaccaac agacaaagcg
    46861 aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc cgtgccacac
    46921 tggccccgac accacgctca ccgtagggag gatagcgcgt ggcagacacc acctgacgtg
    46981 cctgatcggc agtatcgacc atcgggatca gtagagtttg cgcgccaata tccaggactt
    47041 gtttaatcag cgacttactg ccttccaccg gacggatcac gggttggctg gcatagggcg
    47101 ctaccgcctg tagctggtga taaagatcct gaatggtgtt tggcgcgtgc tccccgtcaa
    47161 tcagtaacca gtcataaccg gaagtggcgg caatttctgc catataagaa gtagtcgagc
    47221 ttaaccacag accaatttgc acttcgccct tgcgtaaacg ttctttaaag ggattggtta
    47281 ataatgcgtt catcgtgttc cttaacctat taatgatgtg ccacgtcggt ctgcgcaaca
    47341 gtgcgattta cacgcagagt gaaaataatc agcgaaccga tgaccgccac cgctgccagc
    47401 gtcagtaatc ccgccgcatc gctggcaaat aacgtttctg ctttcacgcg caggatcggg
    47461 gcaataaacc cgcccactgc accaaacagg tttacaaaac caatccccgc agccagcgcc
    47521 gtaccggaaa gcagttgtgt cggcatcgtc cagaacaccg gctgcacggc aataaacccg
    47581 atggctgcaa cacacagcgc tacgatcgcc attactggag aaagcagccc ggacagacca
    47641 ataccaatgc ccgccgccag taatgtcagc gccgcgacat tacgccgttc gccggtttta
    47701 tcggaatagc gcggaataag ccaggtccca aacaatgccg cgacccacgg aatggcggtg
    47761 accaccgacg ccgtaaagcc cacttttgtc cccaacaatg cagcaacttg cgtcggcagg
    47821 aagaaaatta atccgtaaac cgccacctga atggtcaggt agataatcgc cagttgccag
    47881 actcgcccat tacgcagcgc atcgctcagt cgagaagtca ctttctgttg ttcttcactt
    47941 gccagttgat tgataagcaa ttttttttct tgtttactca gaaaacgtgc ctgctccggt
    48001 gtgtcatcaa gccaaaagaa tgtgaatacc ccagcgccga ctgccagcaa tccttcaatc
    48061 acaaacatcc agaaccagcc gggatgcccc ataaatccat gcatctccag cagcgcgccg
    48121 gaaagcggtg atcccagtgt taacgccagc ggtgcgccca tatagaacag ccccatgatg
    48181 ctggcgcgat tacgctgcgg aaaccattgc gaggtgagat aaatcatacc agggaaaaac
    48241 ccggcctccg cagcaccaag cagagtgcga acgatcagaa atttcgcttc agtatcggcc
    48301 catgccatgg ctgccgaaag aaatccccac agcagtgttg tcgtgccaat ccaggttctt
    48361 gcccccagtt tgcgcatcaa aagattcgcc ggaacaccca gaaacgcgta taccacaaag
    48421 aaaatgcctg ctcccagcgc ataagcttca ttactcagcc cggtatcaat ctggtaggtt
    48481 tgtttggcaa aaccgatatt cgaacggtcg agaaacgcca gcacatacag cgccaacata
    48541 aacggaatta aacgcgcacg gtttttcttc accacggcat caagcaaagc ggtgctcata
    48601 aaaagctcct tagaatatgt gggcattacc gttgtcacag caacgcccgg agtgattagt
    48661 ggctgtaggg gcgtttcaat ttgcagtcac gattgagttc gacgccgaaa ccaggtttat
    48721 caagcactga tttatgaata cgaccattca ccggaaccgg ctcattaagc agaatcggat
    48781 caaattgcgg acgcatcgtt gaacaatccg ggctggtcat caggaattcg ttgaatggcg
    48841 tattggtgaa ggtgatcacc gcatggtgag agtaaacaga cgaaccgtgc ggcaccacca
    48901 gttgccctct ggatttggcg attgcggcaa tttccaccag cgtggttaag ccaccgcacc
    48961 agccaacatc cggctgcata atgtcgatac cggtttctga aagcgtacgg aaagattgca
    49021 gtgtgccgtg atgctcaccg ctggtgacca tcattccgac tggcgcgttg cgtttcagtt
    49081 cgcgataacc ttcatactgc tgtggcggca ggcactcttc gatccatttc aggttatagg
    49141 gcgcgcaagc gtgggccagc ttggtggcat agttcacgtc ctgactcatc cagcagtcga
    49201 gcattaacca gaaatcctca ccgcattttt cacgcatatc cgcgaccata gcggcatctt
    49261 tgcggatccc cgcatcgcca tcatgtggcc cccagtgcgt cggcattttg ccaccgataa
    49321 agcccatctc ttttgccaga tccgggcgcg cgcctgtagc gtagaactga atctcatcac
    49381 gaactgcgcc gcctaaaagt ttataaaccg gaagcccgac cactttgccg aacagatccc
    49441 acagagccag atcgacacag gaaatcgtat tcatcaccag gccaccagag ccggagtaat
    49501 acagggtggc attgagcatt tgatcgtgga tcagtttgat atcactgacg catttaccct
    49561 caatgaaacg gttaagatgt ttttcgacaa taaaacagcc catttcaccg gctgtcgaaa
    49621 cagcgaatcc ggtctggccg ttttctgctt caacttcaac aatcaacgtg ccaagaacgt
    49681 taatgccaaa tgactggcgg gactgctcgt attcgcggta tttactcatc ggggtggcaa
    49741 tatgatcatc aatccagtga tttgccccct ggtcgtgata atcaccgccg ccagcgcctt
    49801 tttctgctgt cgcaccgcca gtaaaccagg cgcgaacctg tttgattttt ggtagggtca
    49861 tgatgttctc cattgttatg aggcctgtaa gtcaaaggga cttttccatc ccaacagacg
    49921 tgaaatatcc ctggcgcagg caatggcctt gcccgccaga taatcacggt attcttcatt
    49981 gatttgtaag cgggtaccga ccaccgaaat tgcagcggta agctcgttat tggcgttaaa
    50041 caccggggca gcgacacaac ggacatcggc ataatcttcg ccgttgtcat aactccagcc
    50101 ctgacggcga atacgcgcca gttcttcgtg aagttgctgt ggatgagtaa tcgttgtggg
    50161 tgtcgcctgc tcccagacca gcccttcgat aatactttgc tgtacagctg caggttgcca
    50221 ggcaagcagg catttaccta ttccggagcg ataaagcgaa aggctttttc cttcatggga
    50281 acgcacgctg atagtggctg atgactccac tttcaaaatg taataagcgc tgccgttatc
    50341 aataatcccc aggtgacata acagcccggt ggtatccatc agttgcgtca aacgcggtcg
    50401 agccagttcc cggagatcca tcttgcttaa cgcatgaccg gaaagctcca ccagcttggt
    50461 ccacaaacaa aaattttcct ggtgatcaag gctaagaaaa cgctgacgcc tgagttcatt
    50521 aagcagcaaa taggccgtgc ttttagggat ccccaatgtg tcaatgatcg tcgcagcact
    50581 acaggggcca atgcgggcaa tcaaattgag aatatcaata gcccgtgtca gggcgggaac
    50641 tttgcttgat tccaacatac tggactccag tcttagatac tggaatcagt ctcgttgctc
    50701 agggggagta aaattgtgaa gcgcatcaaa tcccactcct cgtccagcgg actggagaaa
    50761 ttacgcacaa atttgtgaat cagcgcacgg attttttctt attgagtaca acaggttgga
    50821 caaatttcgc cggaggggga atgtgccggt gcagtaaacc ggcacagagt ggcggaatca
    50881 gacagataaa gtcatggact caacaacctc aatccagcca tgctcactgg cgatgtcctg
    50941 accgtttaac caacggcgca gcatattcag tgccatcatt gcgcacactt cctgacggat
    51001 agccaggctg tagcgtgtag tgctgaaacg tacacgcaga gcgaaagtgc catctggcgt
    51061 ggctagcgca aagttgagat gctcgttctc gaaacccgaa acagccagtg ccagcccggc
    51121 aaaatggttg gcccgccgtt ctgtaatcca gtgcgcagtt tgcgccaggg tttcttcctg
    51181 tgaaggaacc acttcacacg ccagcaatgg agcacctgcg cgagaaagtt gcaaagccaa
    51241 taaaccgctg gtgaattgct cgctcaacgt caggctgaac tggcggtttt gcaattcgcg
    51301 actgatctgc gcgggcagtc cttcggtgcc ttcgaaaatc acgctctgtc cggcaacacg
    51361 tttaacgtcc agccacagtt tttccatagc ctgttgctcg cttgccggtc cggtgagttt
    51421 cagttcgata atcggcatag aagagcgata gcccattgtt acgcccggcg gcaattgtag
    51481 agtgtccagg ctttgtgcca gatcgctttc cgaacgacca aaggtagtca aacgcaggca
    51541 aaccggcggc tgcggtaaag aaaaacgctc gcgcaggcgc ggcaggattt cgtgctcgac
    51601 catcacctta aattctgacg gtacgccggg ggtaaagaac atcaggcaac gattaagctg
    51661 cacggcaaaa ccacaggcgg tgcctaccgg gttattgata aattcagcac tggcaggtag
    51721 ctccgcttgt ttacggttgc tcggtgccat tactcgtcca cgttcgtgga aatagcgttc
    51781 catctctttg agccaggctt catgcaacac caggccttca ccttttgctg tcgcagcggc
    51841 gagtgcactt aaatcatcac tggtcggccc cagcccgccg ttaacgatca gcacatcggc
    51901 gtgctgacta cgttcgcgaa gaatggtgac taagtcatca aggttatccc ccaccgtatt
    51961 gcggcgagtt aaaggcaacc cctgatgaaa gaaaaaatcg gccagccagg cagcgttagt
    52021 gtcaacgatt tgcccgtgta acacttcatc cccggtggat aacatttcca cttttaacat
    52081 ggttatctcc tgcttttgtt atcgaatcac tataacgcaa agggagataa ggcgtagtaa
    52141 gaaactgaat ggcgcggcag aatgccgcgc cggaagggat cagaaactgg cgttaacccc
    52201 aacatacggg ccgtcagcca ctgcgttgtc gcggttaccg tctttaccag aaaggttcag
    52261 gtagcgataa cccgcctcaa tgctgactgg acgcataatg gtgtaacgcg cgccagcatt
    52321 cgcttcctca taactttgaa taccgctgga gagcgaatcc ggagagtagt aatactcgcc
    52381 aaacagacgg aagctgttgc caattttcca ctgcaaacca cctcctaccg ctgcggcata
    52441 accttcatcg ccgtaattcg ggttggtgta aacgcctttt ccgccaacgg tcgccattaa
    52501 cggcccgaga ggtagattca accccagccc cacgcccgcg acgtcaccgt cgtcgtcgtt
    52561 atgtgtccag ttaccgctta acgccaggcc cgttgattca gtcccaaaac cgacaccaat
    52621 attggtgtat tctttacccg cctggccgct gatgctcatt gcattgaccg atgcagaaac
    52681 caacagcatt ccggccagac ctgctagcgc aatttttttc atttcccctc tcccacaagc
    52741 aataaatcaa aattcccaaa gctgcgggat tgtactgcct ggcacagagg attcaatgag
    52801 cgaaaaaagg gatctagtca cttattgtaa ctaaatactt cttaaataat taaacgccga
    52861 aaaaataaac atactttatc gatacacaca aaaataataa taagtgccgt tatcatcgca
    52921 aaagagatta ctgtcacttt cacatatttc tgagttaccg tattctcatg gtttcttttt
    52981 atttataagg aatacccctg tgcgacaacg gactattgta tgccctttga ttcaaaatga
    53041 tggtgcttat ttgctgtgta aaatggccga cgatcgcggc gttttccccg gtcaatgggc
    53101 gctttcgggt ggcggcgtag agcctggcga acgaattgaa gaggcactac gccgcgaaat
    53161 tcgcgaagaa ctgggagaac agctgctttt gacagaaatc acgccgtgga ccttcagcga
    53221 tgatattcgc accaagacgt atgcagatgg tcgcaaggaa gagatttata tgatttacct
    53281 gatttttgac tgcgtttctg ccaaccgaga agtgaaaata aacgaagagt ttcaggacta
    53341 cgcgtgggta aaacctgaag atctggcgca ctatgatttg aatgtcgcca cccgaaaaac
    53401 gttacgtttg aaaggtcttc tgtaacaatg atgacagcca ttactggctg tcagattgaa
    53461 aatttagtgg ttaacgaatt ccccatccag ataaactttg cctttttcca catgcatgac
    53521 taaaccatcc agataatcag gtttaaatgt cgcatcacgc ttatctttag caatatatgt
    53581 caggcaatga ttatgggtga aaataacgat atttttatca ggcgctttgc tttgtaagtc
    53641 cttaattgca ctataaatct cattaccgca ctgaagaagt cgtttatcta ccgtcagctt
    53701 tttacccgct gaaaaccagg tcgccgactg aatggtccgg acggtattac tggaataaag
    53761 atcgaaatca gggatatcag cactaaaagc gttgcccagt tcacgggcat cctgggtacc
    53821 tttaaccgta atacctgttt tatctgacaa gcattggttg gttgaacgat cgcaacgttc
    53881 agcatggcga aacaaaacga caaccggatg ctgctgtgcc agtctggcca gcgttttatt
    53941 gtcgatacgt ggcaaaccat tgctactcca ggcggcatga gtacccagtc cggcaattgc
    54001 agcgagcgcc agtaaaatga tgaaatattt ttttgacttc aacgaagagc ggcaaaaagc
    54061 taacacgtaa actccaccta tagacaagcg caaccagaca attaccgtga aattgagcga
    54121 catttctggc gataattagc agttggtgta atattaaaaa tcctgcgatg ccggcaaaat
    54181 gcctcaaaat tttgccaaat gcaaagccta aataagaaaa aatataaaaa tttcaatatt
    54241 tacgtctaat attagtttct taaggttaag ttaatattct atccttaaaa ttttgctcca
    54301 aatggcaaaa tatacacaac actctttata gcaaatataa gtggacaggt attcaatggc
    54361 ggaaggaaaa gcaatgtcag aatttttgcc tttttcgcga ccagcaatgg gcgtggagga
    54421 actcgctgca gttaaagagg ttctcgaatc cggttggatc acaaccggtc cgaagaatca
    54481 ggcgcttgaa caagcctttt gccagttgac gggaaatcag catgccatcg cggtcagttc
    54541 agccaccgcc ggaatgcata tcacgctaat ggcgttggaa attggcaagg gcgatgaagt
    54601 gattacgcct tccctgacct gggtttcaac cctcaatatg atttccttgt tgggtgcaac
    54661 gccggtaatg gtggatgtcg accacgatac gctgatggtc acgcctgaag cgatcgagtc
    54721 agccattacg ccacgcacta aagccatcat tccggtgcat tatgccggtg cgccagcaga
    54781 tattgacgcc attcgcgcca ttggcgaacg ttacggcatc gcagttatcg aagatgctgc
    54841 ccatgccgtc ggtacgtatt acaaaggccg acatattggc gcaaaaggta ccgctatttt
    54901 ttcttttcac gccatcaaaa atattacctg tgcggaaggt ggcctgattg ttacagataa
    54961 tgaaaacctt gcccgccagc tacggatgct aaaatttcac ggtctgggtg tcgatgccta
    55021 tgacagacaa acctggggcc gtgcaccgca agctgaagtc ttaacgccgg gctataagta
    55081 taatctgacc gatattaacg ccgcgattgc cctgacccag ttagccaaat tagagcacct
    55141 caacacccgt cggcgcgaaa ttgctctgca atatcagcaa gcactggcag ctctcccctt
    55201 tcagccatta agccttcccg cctggccgca cgttcacgcc tggcatctgt ttattattcg
    55261 tgtcaatgaa caacgttgtg gtatcagtcg cgatgcgttg atggaagcgt taaaagaaag
    55321 aggcattggt accgggttac atttccgcgc cgctcacaca caaaaatatt atcgcgagcg
    55381 ttttcccacg ctgtcgttac cgaataccga atggaatagc gaacgcatct gttcgttgcc
    55441 gctgttcccg gatatgacca ccgccgatgc cgaccgcgtc atcacagccc ttcagcaact
    55501 cgcaggacaa taagccatgt ttgaaatcca ccctgttaag aaagtctcgg tggttattcc
    55561 cgtttataac gagcaggaaa gcttaccgga attaatcagg cgcaccacaa aagcctgtga
    55621 atcactgggg aaagagtatg agatcctgct gattgatgac ggcagtagcg ataattccgc
    55681 acatatgctg gtcgaagcct cacaagcgga gaacagccat attgtgtcta ttttgcttaa
    55741 ccgcaattac gggcaacatt cagcgattat ggcgggattc agtcacgtta ctggcgactt
    55801 aattattacc cttgatgccg atctccagaa tccgccagag gagatccccc gcctggtggc
    55861 aaaagccgat gaaggctacg acgtggtagg aactgtgcgt cagaaccgcc aggacagctg
    55921 gtttcgtaaa acggcatcga agatgattaa ccggcttatt cagcgcacca ccggcaaagc
    55981 gatgggtgac tacggttgta tgctgcgcgc ctatcgccgt catattgtcg atgcgatgtt
    56041 gcactgccat gaacgcagca cctttatccc gattctggcg aatatcttcg cccgccgtgc
    56101 cattgaaatt ccagtgcacc atgccgagcg tgagtttggt gaatccaaat acagttttat
    56161 gcgcctgatt aatttgatgt acgacctggt gacctgcctt accacaacgc cgctacgtat
    56221 gctgagtctg cttggcagca ttattgcgat tggaggtttt agcattgcgg tgctgctggt
    56281 gattttacgc ctgaccttcg gaccacaatg ggcggcagaa ggcgtcttta tgctatttgc
    56341 cgtgctgttt acttttattg gcgctcagtt tatcggcatg ggattgctcg gtgaatatat
    56401 cggcaggatc tacaccgatg tccgcgcccg cccccgctat tttgttcagc aagttatccg
    56461 tccatccagc aaggaaaatg aataatgaaa accgtcgttt ttgcctacca cgatatggga
    56521 tgcctcggta ttgaagccct gctgtctgcc ggttacgaaa ttagcgccat ttttacccat
    56581 accgataatc ccggtgaaaa agccttttat ggttcggtgg ctcgtctggc ggcggaaaga
    56641 ggcattccgg tttatgcgcc ggataacgtt aatcatccgc tgtgggtgga acgtattgcc
    56701 caactctcac cagaggtgat tttctctttt tattatcgcc atcttatttg cgacgagatt
    56761 ttgcagctcg ctcccgcagg tgcatttaat ctgcatggtt cgctgttacc aaaatatcgt
    56821 ggtcgcgcgc cgctgaactg ggtgctggtg aacggtgaaa cggaaactgg cgttacattg
    56881 caccgaatgg tgaaacgtgc cgatgccggg gccattgtgg cgcaactgcg cattgccatt
    56941 gcgccagacg atatcgctat tacgctgcat cataaattgt gccatgccgc gcgccagcta
    57001 ctggaacaga cattacccgc cattaaacac ggtaatattc tggaaatcgc ccagcgcgaa
    57061 aacgaagcca cctgttttgg tcgcagaaca ccggaagaca gtttcctcga atggcataaa
    57121 ccggcatccg tgctgcacaa catggtgcgt gccgttgccg atccgtggcc gggtgccttc
    57181 agctatgttg gtaatcagaa atttaccgtc tggtcgtcac gcgtgcattc tcatgcgccc
    57241 gcagcacaac cggggagcgt gatttctgtt gcgccactgc tgattgcctg tggcgatggc
    57301 gcgctggaaa tcgtcaccgg acaggcgggc gacggcatta ctatgcaggg ctcgcaatta
    57361 gcgcagacgc tgggcctggt gcaaggttca cgcttgaata gccagcctgc ctgtgccgcc
    57421 cgacgccgta cccgggtact catcctcggg gtgaatggct ttattggcaa ccatctgaca
    57481 gaacgcctgc tgcgcgaaga tcattatgaa gtttacggtc tggatatcgg cagcgatgcg
    57541 ataagccgtt ttctgaatca tccgcatttt cactttgtcg aaggcgatat cagtattcat
    57601 tccgaatgga ttgagtatca cgtcaaaaaa tgtgatgtcg tcttgccgct ggtggcgata
    57661 gccacgccga ttgaatatac ccgcaaccca ctgcgcgtat ttgaactcga ttttgaagag
    57721 aatctgcgca ttatccgcta ctgcgtgaag taccgtaagc gaatcatctt cccgtcgact
    57781 tcagaagttt atgggatgtg tagcgataaa tacttcgatg aggatcattc taatttaatc
    57841 gtcggcccgg tgaataaacc acgctggatt tattcggtgt ctaaacaatt acttgatcgg
    57901 gttatctggg cctatggcga aaaagagggt ttacagttta ccctcttccg cccgtttaac
    57961 tggatgggac cacgactgga taaccttaat gcggcacgaa tcggcagctc ccgcgctatt
    58021 acgcaactca ttctcaatct ggtagaaggt tcaccaatta agctaattga tggcggaaaa
    58081 caaaaacgct gctttactga tatccgggat ggtatcgagg cgttgtaccg cattatcgaa
    58141 aatgcgggaa atcgctgcga cggcgaaatt atcaacattg gcaatcctga gaacgaagcg
    58201 agcattgagg aactgggcga gatgctgctg gcgagcttcg aaaaacatcc gctgcgccat
    58261 cacttcccac cgtttgcggg ctttcgtgtt gtcgaaagta gcagctacta cggcaaagga
    58321 tatcaggacg tagagcatcg taaaccgagc atccgcaatg cccgccgctg ccttgactgg
    58381 gaacccaaaa ttgatatgca tgaaaccatc gacgaaacgc tagatttctt cctgcgcacc
    58441 gttgatctta cggataaacc atcatgacca aagtcggctt acgcattgat gtcgatacct
    58501 ttcgtggcac ccgtgaaggc gtgccgcgtc tgctggaaat cttgagtaag cataatattc
    58561 aggccagcat ttttttcagc gtcggcccgg acaatatggg ccgccatctc tggcgactgg
    58621 tgaagccaca gtttttgtgg aagatgctgc gctcaaacgc ggcatcgctt tatggctggg
    58681 atattttact ggcaggtacg gcctggccgg gtaaagagat tggtcatgcc aatgccgata
    58741 tcattcgtga agcagctaaa catcacgaag tcggcctgca cgcctgggat caccatgcct
    58801 ggcaagccca tagcggtaac tgggatcggc aaacattggt cgacgatatt gcacgcggcc
    58861 ttcgcaccct ggaagagatc ataggtcaac cggtaacctg ttctgccgct gcgggctggc
    58921 gtgccgacca gcaggtgatc gaagcaaaag aagcgttcca tttgcgctac aacagcgatt
    58981 gtcgtggggc catgccgttt cggccactgc tcgaatcagg aaaccctggc actgcgcaaa
    59041 ttccggtgac cttacccacc tgggatgaag tgattggtcg ggatgtgaaa gcagaagatt
    59101 ttaacggttg gttactcaac cgcatcctgc gagataaagg catgccggtt tataccattc
    59161 atgcagaagt tgaaggctgc gcttatcagc ataattttgt ggatctcctg aaacgggcag
    59221 cccaggaagg cgtgacattt tgccctttaa gcgaactgtt atcagggacg ttgccgctcg
    59281 gacaagttgt tcgcggaaat attgccggac gtgaaggctg gctgggttgc caacaaattg
    59341 cgggtagtcg ttgatgaaat cggtacgtta ccttatcggc ctcttcgcgt ttattgcctg
    59401 ctattacctg ttaccgatca gcacgcgtct gctatggcaa cccgatgaaa cgcgttatgc
    59461 ggaaatcagt cgggaaatgc tggcatccgg cgactggatt gtgccccatc tgttagggct
    59521 acgttatttc gaaaaaccca ttgccggata ctggattaac agcattgggc aatggctatt
    59581 tggcgcgaat aactttggtg tgcgggcagg cgttatcttt gcgaccctgt taactgccgc
    59641 gctggtgacc tggtttactc tgcgcttatg gcgcgataaa cgtctggctc tactcgccgc
    59701 agtgatttat ctctcattgt ttattgtcta tgccattggc acttatgccg tgctcgatcc
    59761 gtttattgca ttctggctgg tagcgggaat gtgcagcttc tggctggcaa tgcaggcaca
    59821 gacgtggaaa ggcaaaagcg cgggattttt actgctggga atcacttgcg gcatgggggt
    59881 gatgaccaaa ggttttctcg cccttgccgt gccggtatta agcgtgcttc catgggttgc
    59941 atcgcaaaaa cgctggaaag atctctttat ttacggctgg ctggcggtta tcagttgcgt
    60001 actgacggtt ctcccctggg gactggcgat agcgcagcgg gagcctgact tctggcacta
    60061 ttttttctgg gttgagcata ttcaacgctt tgcaatggat gatgctcagc atagagctcc
    60121 gttctggtac tacttgccgg tcatcattgc cggtagcctg ccgtggctgg gattactccc
    60181 cggagcactg tacgcaggct ggaaaaaccg caagcattcc gcaaccgtct atttgttgag
    60241 ctggacgata atgccgctgc tgtttttctc cgtcgctaaa ggcaaattgc ccacctatat
    60301 tctttcctgc tttgcacctc tggcaatgct gatggcgcat tacgctttgc tggcagcaaa
    60361 aaataatcct ctggcgctgc ggattaatgg ctggattaac atcgcttttg gtgtcactgg
    60421 cattattgcc acatttgtgg tctccccatg gggaccaatg aacacgccag tgtggcaaac
    60481 cttcgagagc tataaagtct tttgtgcctg gtcgattttt tcgctatggg catttttcgg
    60541 ctggtacacc ttaacaaacg tcgaaaagac ctggtctttt gccgcgcttt gcccgctggg
    60601 gctggcgttg ctggtaggat tttcaattcc tgacagagtc atggaaggaa aacatccgca
    60661 attttttgtc gagatgacac aagaatcact tcagccaagc cgctatattc ttactgatag
    60721 cgtcggcgtt gccgcaggtc tggcatggag cctgcaacgc gatgacatca tcatgtatcg
    60781 ccagacaggt gagttgaaat acggccttaa ttatccggat gcgaaaggga gatttgtcag
    60841 cggtgatgag ttcgcaaact ggcttaatca acatcgtcag gaggggatta ttacactcgt
    60901 gctttcggtt gaccgcaatg aagatatcaa cagtctcgcc attccgcccg cagatgccat
    60961 cgatcgtcag gagcgtctgg tgctgattca gtatcgtccc aaatgatctg gctaacatta
    61021 gtctttgcca gcttgcttag cgttgccggg cagttgtgtc agaaacaggc aacctgcttt
    61081 gtggcgataa acaaacggcg caaacatatc gtgctgtggc tgggactggc gctggcttgt
    61141 cttggtcttg ccatggtgct ctggctgctg gtcttgcaga acgtaccggt aggcattgcc
    61201 tacccgatgt taagtctgaa ttttgtctgg gtgacgctgg ctgcagtaaa actgtggcac
    61261 gaaccggtat cgccgcgtca ctggtgtggg gtggcgttca ttattggtgg cattgtgatc
    61321 ctcgggagta ccgtgtaatg ggcctgatgt ggggattatt cagcgtcatt atcgcctccg
    61381 ctgcgcaatt aagcctgggt tttgcggcga gtcatctgcc gccgatgacg cacttatggg
    61441 attttatcgc ggcgctgtta gcttttggcc tggatgcccg gatactgtta ctcgggctgc
    61501 tgggttatct gctttccgtt ttttgttggt ataaaacact acataaactt gcactaagca
    61561 aagcctatgc cttgctcagt atgagttatg tgctggtatg gattgcttca atggtacttc
    61621 ccggctggga agggactttt tcgctgaaag cactactggg agtagcctgt attatgagcg
    61681 ggttgatgct gattttcctg cccacgacaa aacaacgtta ctgagttttc cctgccactt
    61741 tacactgccg ttcccactca tcggggctat aactgcttgc cgagaggact ttcaccgtat
    61801 gcagcttttc acgattaata caataaagcg cattctgcaa aggcgagagt aaatccccca
    61861 ctttcacggc gaagtcactc ttcacttcgg cgatcatttt tatcgtgcca ccagcatcac
    61921 aaagcttaag tacatgtctg ttatcttgtt tatcacagca tgacttttta accagccatt
    61981 ccattgcacc accctgcttg ataagaattt tcacattaat ttacagatta acagataaac
    62041 ctattatcaa cggcaatgga atggcaaaat tgcgaagtga aactaatgat ttcttaatcc
    62101 tggcgttgca cccactcttt tagcgcctga cgtgaaatct taatgccgcc gtttttcagt
    62161 tcaggcggca gcgttaacca acgcaccggt tgctgaaaac gggccagctt atctttcacc
    62221 cattcactaa gatcaacggt ttgctggtca tactccacca ccgctaccgg ccgatgacca
    62281 aactccttgt cggcaacggg gacgataaac acctgcaaaa ccgcaggatg tgcagcaata
    62341 acgcgctcga cttcttccgg ctgaataccc tcaccgccac tgaagaacag attgtctaaa
    62401 cgcccgacaa tggtcagctt gccattatgc agctcaccgc ggtcgcgcgt agcgtaccag
    62461 ccttcgtcat taaccagtgc caccagttgc ccgttacgcc agtaaccttc agccatactg
    62521 gcagcccgca gccacacttc atcattaacg attttcactt cccgacccgg cagcggcgaa
    62581 ccaacgtctg ccagaccgtc ggcttctttc gcacacaccg tggaggcaaa ctcggtcaga
    62641 ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct gttccgtcaa ctcgaccggg
    62701 atagccgcgc cgccaagtag caccgctttc agggaaacgg aactacggtt aaccagcaaa
    62761 cgccagagtt gtgttggcac cagtgaagcg tgagtacagc ctgccagcat ttgctccaat
    62821 ggctgtttat cacgtaccgt catccgcgca ccagcgtata accagcgcca cataattccc
    62881 tgaccggaga cgtgaaacag cggtaaagag agcaaccagt catcgtgctc gccaaacggt
    62941 atcagcgata acacaccttg cgcactggca agatgggcct gataagtatg aacagcggct
    63001 ttcggcaaac cggtagagcc ggaggtcaac gtcattgagc acagacgcgt cggctgccac
    63061 gcagcggcat gtgcgccttc aaccagctga atgtgcagcg acgttaacgc cggaaacgtg
    63121 ttttccccat ccggcaccag cgcaaattgc agcgtcagat tgggcagcaa ttcttcaagc
    63181 aacggttgcg gcagctgagg gttcacgggc aacacccgcg ccccgcattg cagtaacgcc
    63241 agccaggcga gcagcgtttg cggcgtattc cacgcccgca acatcacgcc gctgccctca
    63301 acaacgccct gcgccgcaaa gccggaggct aattcatcga cgcgagcaca aagctcgcgc
    63361 cagttaagtt gctcgtcatt aagacgtaag gcgatggctt ctccccgcac ttgccgccag
    63421 tgacgccacg gccagtcaga gaagatcata acagccgctc cagtgcatca acttccacta
    63481 acggtagcgg gctacccggc cagcgacgaa cctgctgcgc ctgcatcaga tccagcgtgt
    63541 ccagccccgg aatggtgtct ggcgttaacc aggcggcaat ccgcgccagt tgcgttaagc
    63601 ctaagctcga ctcaatagaa gaactgatca ccgccgtcag ccccaacgcg tgcgccgcct
    63661 gcacctgctc acgtactttc tccaggctgc ccgtgagcgt aggtttgata actaccgcgc
    63721 tcacgccctc ttcagccaca aaggcaaaat ccggctcgcg caggctttca tcccaggcaa
    63781 tggcaatgcc ggtttcacgg gcaaacgcac gggaatcatc gcgggttttg cacggctctt
    63841 cgagaaacgc gatgcggtgg cgataatccg ggttaacgta tttggcaaac tgctgacctt
    63901 tcagcgacgt ccaggcgcga tttgcgtcaa gacgcaaatg cagatccgga attgcctcca
    63961 gcaacagatt caccaccatg ccgtcgcgca ccgcttcgta caatcccact ttgaccttcg
    64021 ccactttctc gcccggcata tcggcaagtt tgaggatcag atcgtccgga tcgccattac
    64081 acagcggtgc cgcacggtag ttggctgctt gcggcaacgc atcggtcagt tctgccaatg
    64141 cacagcttac gccaaaggcc acggaaggca tctgcggtag ctcgcaatcg cctgccagcc
    64201 agttatttac ccaggcaagc agcacgcttt gcgcctcttc ccaggtttcc tggctgaagc
    64261 ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc ctcacgcagg caaacataca
    64321 gcccgtcgcg ggtttttaac cgcctgtcgc gcagaaccac ccccgcgtcc atggggatct
    64381 gccagcggta tacctgcgcg ctacgcatta cggattccgt ttaaatttgc tgaaatcagg
    64441 ctgacgtttc tggttgaaag cgttgcgacc ttcctgacct tcttccgtca tatagaacag
    64501 catggtggcg ttacctgcca gctcctgcag acctgcctga ccgtcacagt cggcgttcag
    64561 cgcagctttc aggcagcgca gcgccatcgg gctgttttgc agcatttcgc ggcaccaacg
    64621 gacggtttct ttttccagat ccgccagcgg taccacggtg ttcacaaggc ccatatccag
    64681 cgcctgtttc gcgtcgtact gacggcacag gaaccagatt tcacgcgctt ttttctgtcc
    64741 gacgatgcga gccatgtagg aagcgcccca gccgccgtcg aaggaaccga ctttcgggcc
    64801 agtctggccg aagatggcat tatcagccgc gatagtcagg tcgcacatca tgtgcagaac
    64861 gtgaccgccg ccgatggagt agccagccac catcgcgaca accggtttcg gacaggtacg
    64921 aatctgacgc tggaagtcca gcacgttcag gtgatgtacg ccggaatcat ctttatagcc
    64981 gccgtaatca ccacgcactt tctggtcacc accggagcag aacgctttat cgcctgcgcc
    65041 agtcagaatg atcacgccga tgttgtcgtc ataacgcgca tctgccagtg cctggatcat
    65101 ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc ggacgattaa tggtgatttt
    65161 tgcgataccg tcggcagatt tttcataacg aatgtcctcg aaaccttcgg agcagtcgtg
    65221 ccattcaacc ggtgcgtaaa gcattgcttc atcaggataa atcatattgt gtcctttagt
    65281 cagaaacgca agatctgcgc cagacttgcg attacgccag cgggattttc ccgatgcgcg
    65341 ttatgtccgg cgcgaggaat gacatggcat tcggcagcca gttccgccgc cagggcgcgg
    65401 aatttgctgt cacgttcacc acataaataa taaaacgcaa atgtgcgggc gctaaggtta
    65461 gcacgtaaat cgggctggac ggcgagagaa gtcgcctcca gcatggcggc aagcgttgcg
    65521 ccattattgt tgctgcgcag cgcaaccagc tcccggcgtt gttcgtcatt gagtgaggca
    65581 aaaacaggct gttgatacca gtcagcaaat accgccgtta acggttctgt gcgaaaacgc
    65641 tgcgcccatt ggcgatcgga acgctgacgt tccgtgcgtt gttcagcatt ttgcaaccca
    65701 gggtgcccgc cttcgacaat aaccccacaa agccccggca tcccctgaca agccgccatc
    65761 atcgccaccc gtccgccaag cgagtacccc accagccaga agttaaggat gttgtaacta
    65821 accagagttt tgcgcagtaa gccggtgaca tcatcaaatc catcgacgcg aatcgccgcc
    65881 gaaccaccgt gacctgggag atcaacatac aaccgcgagt agttggcaaa cacctcgccc
    65941 acttcttgcc attcatggca atcgccggaa aaaccgtgca aaaacaccag ccagggtaaa
    66001 cctggtttac cgtgttttgc ctgcgcgtgc aggatcataa atggctaacc tgcgccagca
    66061 gttgctggag ggtttgcgca ccattggtgt cgttaaccac catttcaatt accgtggtgg
    66121 tcggcgtgcg ccaggcgtcg gcaagtgccg tttcaagttc ctgccagttt tgcggacaat
    66181 gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg gacgttttgc ggcatcagat
    66241 agaaacgctc gcgctcgttt ttcggcgttg gcaacagcga gaaaatttgc ccgccattgt
    66301 tgttcaccac aattaatacc agcggcgcgg aaacctgacg taataacgcc agcgcgttga
    66361 gatcgtaaag tgctgagaga tcgcccacaa tcgccagcgt cggtttgcca ctggcccgct
    66421 gaacgccagc agcagttgag agcagcccgt cgataccgct ggcacctcgg ttgctgtaca
    66481 ccgggtaacc tgccggaagt tgcgaaagcg catcaatcag acgtaccacc aggctgttac
    66541 cgacaaacaa ttgcccctgt tcaggcaaat agtcgctgat gcgatgcgcc agttgcgctt
    66601 cgccaaacgc atcgcgacgg gcaataaccg cctgcattgc ctgttcagcc aggcgcggga
    66661 tttcaacgca ccagggctgg cgtttttctg ccggatgttg ctccagccag tcggcaatat
    66721 tggcaattaa gcgacgtccg cgatggtgtg ccggatcaag tcgcccttca atgtcatcaa
    66781 caatccagta ctcttcgggt tcacagcttg cctgccattg caggagccgt ttgcccgtca
    66841 ggctgcttcc cagttgcacc acaatttgcg cctgttgcag ttcgctggtc gctttggcat
    66901 tgcctaacca gagatcggca cacggcagcg gctgtccggt ttgcgacagc acatcgccaa
    66961 tcagcggcca gccaagagtt tgcgcccaca gtgcgacttt tttgccctct tccgcactca
    67021 tgcgcccggc aaccaccacg ccgcgctttt gtcgccagaa gaaccagtcg cgctgttttt
    67081 cactttccag acgaggcgct tcacgcaacc acggtttgtc gtcctgccac cagtcgccca
    67141 gacgctgttg ccagctaagc ccggtgtcgt ccatttcgcc atacagcggt tcagcaaacg
    67201 ggcagttgat atggactcca ccagcatgaa gcgtaccgag agcgtggtcg atggtagaaa
    67261 ccagccaacg tgcggggata tcctgggtcg ggcgcggcag tgaaatactg tgcgtggggt
    67321 gagaggcgaa cattcccggc tgacgaatcg cctgattcgc gccgcagtca attagctccg
    67381 gcgggcgatc ggcggttaac aggatcagtt tttctccggt taacccggct tcaatcagtg
    67441 ccggatagag atttgctacc gccgtgccgg aggtcacaat cacagccacc ggctgcttgc
    67501 tgactttcgc cagccccagc gccagatgcc ccagtccacg ctcatcgaaa tgggtgtggt
    67561 gaatgaatgc ggaattctcc gccgccgcta acgttaacgg tgtagaacgc gagccagggg
    67621 cgatacagat gtgtctgacg ccgtgacgcg ttaatgcttc cagaatgacc gccgcccagc
    67681 gtcggttaaa tgcgcttact gacatgagtt tgtccggtat caatattgcg gctaagtata
    67741 aggagctaca aaaatagatt attgatatga atcggtaatg atgcgactca ttactattcc
    67801 atttgtaata aagtacgcag ccctgccgct ttgttgtcga tttcctgcca ctcttgctcg
    67861 gggtcggaac cacggacaat gcccgcgcca gcatatagtc gcacgacatt gccgctaatt
    67921 tttgctgagc gcagggaaac gcagaattcg ctttgttgta aagagagata gcccgcagaa
    67981 ccggcgtacc attctcgggt gaacggttcg tgacgggcga taaactgtcg cgccagatcg
    68041 cgcggtaagc cagcaactgc tgccgttggc tgcaactgat gtaaacagat cacatcatcc
    68101 gctttgttga gtgcagtcca gatacagcgg cgaagatgct gcactttacg cagacgcagt
    68161 acctgcggcg gtaaaacatc cagcgtcagg gtatcggcct gcaatcgttg gcagatatcc
    68221 tccaccacca acatgttctc gcgctggttt ttatcatccg ccatcagcca ctcacctaac
    68281 tgctgcgcct gcttatcatc aggatgattt gctaccgttc ccgccagcgc ttcggtacgc
    68341 agcgctttgt cacgccgccg ccataaccgt tccggtgaag aaccaagaaa agcattttcg
    68401 ccatcaaagg ccatgtaaaa atggtagcaa ttcagattga gccgacgact ggcagccatc
    68461 atcgctgccg cattgaccgg acttgcgaaa tgcaggtcag ttgcccgagc aagcaccact
    68521 ttgtcgagct caccttcggc gatggttttc gttgccagtt cgattaattg cgtccagccc
    68581 gttttgtccg gccagtgttg ttctcgcgtg gtggttaaat gtaacccagg caagggcttg
    68641 atactcacca gtttggcgat aaattctttt gccttgattg catcgtgctg aagggagctt
    68701 tcgctgaata gcgtcaccct cagcgtggct ttaccgccac agcgtcgcca ttcgaggcgt
    68761 ggtaaaagta aattaccctg cgacgggtca aacgcattca gcccccagat gcgtaagtcg
    68821 gcgtgttccg ggtgctggcg aagaaaacgt tgtgcctggt ccaacgacgt aaaacgggta
    68881 atcgcaccca ggacggcagc ttcttcatca ccattacgtt gttgccagta gaattgcgga
    68941 tacgtttgct gactggccag ccagctcaag gcatcaaaag cgtctttgag agggaaagga
    69001 atatcgataa cccgaatgcc gggtgtcgcc ggaatctctt gcgacaaatg gcgcagtaga
    69061 ttttccagcg ccgtagtaag tgattgcacg cggacctctc cctgttaaaa gcctcacatt
    69121 atacggggta ctacaaaaaa aatgcagtac cccggtgtag ggaggtttta acggcgtgcc
    69181 agcaacagtc ctagtaccag cccaacggcc gcgcccacac caattccttg ccagggtttt
    69241 tcgtggacgt agtcatcagc acgataaaca gcctgcttcg cccgatagta ataactgtct
    69301 gaagcctggc taacccgttt tttcacatca tccagcgctt tttctgcacg cgctttcagc
    69361 tcaacatatt tctgatcggc gggatcgcca gaggagcgga gcacctcttc cagtgtttca
    69421 ctaagcagcg tcaggtcatc atcgatacgt gtatcaccaa actgattaga cattctcgtt
    69481 ctccatttgc gtaaaacctg tcactaacta tagacaaggg ttattgatta cgcctgaatt
    69541 acctcgcgtg ccatcccaat gtgtgggata ccatcttctt catagacctc cgtcacaggg
    69601 ataaagccaa aactctggta gaagttttgc agatgcgcct gcgccccgag gtaaacaggt
    69661 ttatcaggcc agtgacgcgt acatgtttcg agtgttttat tcatcagctg ctgccccact
    69721 ttttcgccac gcaacgcttc actaacaatc acccgaccaa taacgaccgg ctcaagatca
    69781 tcatcacttt tcagaatcct cgcatacgcc accagttcat catttttcca accgagaata
    69841 tggcgattat cccccgtcaa atcatcacca tcgatatcct gataagggca attctgctca
    69901 acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa gctgggaaac cgataattca
    69961 gagtgatgca gatcttgcca ttcaatcata atgctctcct tttagatgta tgccttatac
    70021 tagccggaat attttttgaa acgtgatgaa ctcatggaat taattttttt aggtacttca
    70081 gccggtgtgc caacccgtac gcgcaatgtc acggcaatat tgcttaacct gcaacatccg
    70141 acccagagcg gtctttggtt gtttgactgc ggtgaaggca cccagcatca gctactgcat
    70201 accgccttta accctggaaa actggataag atttttatca gccaccttca tggcgatcat
    70261 ctctttggtt tacccggctt gctgtgcagt cgttctatgt caggcattat ccaaccctta
    70321 acgatttatg gtccgcaagg tatccgtgaa tttgtggaga ctgcgctgcg gattagcggc
    70381 tcatggaccg attatccgct ggaaattgtc gaaattggcg ctggcgaaat tctcgatgat
    70441 ggcctgcgca aagtgaccgc ttatccgatg gaacacccac tggaatgtta tggctatcgt
    70501 attgaagaac atgataaacc gggtgcatta aatgcccagg cattaaaagc tgctggcgtg
    70561 ccgcctggcc cactgtttca ggcattaaaa gcgggcaaaa caatcacgct ggaagacgga
    70621 aggcagatta acggcgcaga ttacttagcc gctccagtgc caggtaaagc gctcgctatt
    70681 ttcggcgata ccggcccctg cgatgccgca cttgacctgg ctaaaggtgt cgatgtcatg
    70741 gtgcacgaag cgacgctgga tataaccatg gaagccaaag ccaatagtcg cggccatagc
    70801 tctacacgcc aggctgcgac actagcccgt gaggctggag tcggtaagct aatcattacc
    70861 cacgtcagct cgcgctatga tgacaaaggt tgtcagcacc tgttacgtga atgtcggtca
    70921 attttcccgg cgactgaact ggcgaatgat ttcaccgtgt ttaacgttta actctgctta
    70981 ctactaaaaa cagggcgaaa cttgccctgt tatcgcaacc cgcgctacat atatccaccc
    71041 acacgggaat attgagcgat ttttgatatt tattcatctc ctcacgcact atattcagag
    71101 caataagttg cctcttttta tcttcattca gcacaacctt tgcaactaaa aggagcctat
    71161 gatggttaca gttgtcagca attattgtca attatctcaa acccaactct gtcagacatt
    71221 tgcagaaaaa tttactgtga ccgaggaatt actgcagtct ttaaaaaaaa cagcgttatc
    71281 cggggatgaa gaaagtattg agttactgca taacattgcg ttaggttatg atgaatttgg
    71341 gaaaaaagct gaagatattc tttaccatat tgttagaaac ccaacaaatg agaccctatc
    71401 gattatccga cttattaaaa atgcctgttt aaaattatat aatttggcac
//
LOCUS       DAPOZO010000009        71424 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPOZO010000009 DAPOZO010000000
VERSION     DAPOZO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.009"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1115)
                     /gene="rnd"
                     /locus_tag="ROI21_000631"
     CDS             complement(<1..1115)
                     /gene="rnd"
                     /locus_tag="ROI21_000631"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDX5109596.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(1197..2882)
                     /gene="fadD"
                     /locus_tag="ROI21_000632"
     CDS             complement(1197..2882)
                     /gene="fadD"
                     /locus_tag="ROI21_000632"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDX5109597.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(3087..3668)
                     /gene="yeaY"
                     /locus_tag="ROI21_000633"
     CDS             complement(3087..3668)
                     /gene="yeaY"
                     /locus_tag="ROI21_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDX5109598.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSRTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(3707..4402)
                     /gene="tsaB"
                     /locus_tag="ROI21_000634"
     CDS             complement(3707..4402)
                     /gene="tsaB"
                     /locus_tag="ROI21_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDX5109599.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLALCDGEVLLPAAEDMLPIACQMFAESKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(4460..6370)
                     /locus_tag="ROI21_000635"
     CDS             complement(4460..6370)
                     /locus_tag="ROI21_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDX5109600.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            6502..6846
                     /locus_tag="ROI21_000636"
     CDS             6502..6846
                     /locus_tag="ROI21_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDX5109601.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDTVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            7208..7567
                     /locus_tag="ROI21_000637"
     CDS             7208..7567
                     /locus_tag="ROI21_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDX5109602.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERIVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(7687..7866)
                     /locus_tag="ROI21_000638"
     CDS             complement(7687..7866)
                     /locus_tag="ROI21_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDX5109603.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            7940..9301
                     /gene="pabB"
                     /locus_tag="ROI21_000639"
     CDS             7940..9301
                     /gene="pabB"
                     /locus_tag="ROI21_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDX5109604.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSENEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFALTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     QAAYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSPKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            9305..9883
                     /locus_tag="ROI21_000640"
     CDS             9305..9883
                     /locus_tag="ROI21_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDX5109605.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            10067..11431
                     /gene="sdaA"
                     /locus_tag="ROI21_000641"
     CDS             10067..11431
                     /gene="sdaA"
                     /locus_tag="ROI21_000641"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDX5109606.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVIYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            11562..13160
                     /locus_tag="ROI21_000642"
     CDS             11562..13160
                     /locus_tag="ROI21_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDX5109607.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPSAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVGKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLCDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(13164..14720)
                     /gene="yoaE"
                     /locus_tag="ROI21_000643"
     CDS             complement(13164..14720)
                     /gene="yoaE"
                     /locus_tag="ROI21_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDX5109608.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            15183..16154
                     /gene="manX"
                     /locus_tag="ROI21_000644"
     CDS             15183..16154
                     /gene="manX"
                     /locus_tag="ROI21_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDX5109609.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            16217..17017
                     /gene="manY"
                     /locus_tag="ROI21_000645"
     CDS             16217..17017
                     /gene="manY"
                     /locus_tag="ROI21_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDX5109610.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            17030..17881
                     /gene="manZ"
                     /locus_tag="ROI21_000646"
     CDS             17030..17881
                     /gene="manZ"
                     /locus_tag="ROI21_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDX5109611.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            17936..18394
                     /locus_tag="ROI21_000647"
     CDS             17936..18394
                     /locus_tag="ROI21_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDX5109612.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            18823..19389
                     /gene="mntP"
                     /locus_tag="ROI21_000648"
     CDS             18823..19389
                     /gene="mntP"
                     /locus_tag="ROI21_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDX5109613.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(19386..20195)
                     /gene="rlmA"
                     /locus_tag="ROI21_000649"
     CDS             complement(19386..20195)
                     /gene="rlmA"
                     /locus_tag="ROI21_000649"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDX5109614.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKSEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(20361..20570)
                     /gene="cspE"
                     /locus_tag="ROI21_000650"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(20361..20570)
                     /gene="cspE"
                     /locus_tag="ROI21_000650"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDX5109615.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(20583..20726)
                     /locus_tag="ROI21_000651"
     CDS             complement(20583..20726)
                     /locus_tag="ROI21_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDX5109616.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(21396..21683)
                     /locus_tag="ROI21_000652"
     CDS             complement(21396..21683)
                     /locus_tag="ROI21_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDX5109617.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(21758..21901)
                     /gene="mgrB"
                     /locus_tag="ROI21_000653"
     CDS             complement(21758..21901)
                     /gene="mgrB"
                     /locus_tag="ROI21_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDX5109618.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            22060..22299
                     /locus_tag="ROI21_000654"
     CDS             22060..22299
                     /locus_tag="ROI21_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109619.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(22443..23234)
                     /gene="kdgR"
                     /locus_tag="ROI21_000655"
     CDS             complement(22443..23234)
                     /gene="kdgR"
                     /locus_tag="ROI21_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDX5109620.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTESLLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            23411..24784
                     /locus_tag="ROI21_000656"
     CDS             23411..24784
                     /locus_tag="ROI21_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5109621.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            complement(24830..25711)
                     /gene="htpX"
                     /locus_tag="ROI21_000657"
     CDS             complement(24830..25711)
                     /gene="htpX"
                     /locus_tag="ROI21_000657"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDX5109622.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNIVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(25903..27951)
                     /gene="prc"
                     /locus_tag="ROI21_000658"
     CDS             complement(25903..27951)
                     /gene="prc"
                     /locus_tag="ROI21_000658"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDX5109623.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(27971..28669)
                     /gene="proQ"
                     /locus_tag="ROI21_000659"
     CDS             complement(27971..28669)
                     /gene="proQ"
                     /locus_tag="ROI21_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDX5109624.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(28766..29263)
                     /gene="msrC"
                     /locus_tag="ROI21_000660"
     CDS             complement(28766..29263)
                     /gene="msrC"
                     /locus_tag="ROI21_000660"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDX5109625.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            29393..30676
                     /gene="yebS"
                     /locus_tag="ROI21_000661"
     CDS             29393..30676
                     /gene="yebS"
                     /locus_tag="ROI21_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDX5109626.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQTGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            30645..33278
                     /gene="yebT"
                     /locus_tag="ROI21_000662"
     CDS             30645..33278
                     /gene="yebT"
                     /locus_tag="ROI21_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDX5109627.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            33358..34797
                     /gene="rsmF"
                     /locus_tag="ROI21_000663"
     CDS             33358..34797
                     /gene="rsmF"
                     /locus_tag="ROI21_000663"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDX5109628.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAFYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGIRNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCRWLKETYPDAVEFLPLGD
                     LFPGANKALTEDGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            34915..35151
                     /locus_tag="ROI21_000664"
     CDS             34915..35151
                     /locus_tag="ROI21_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDX5109629.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            35256..35447
                     /locus_tag="ROI21_000665"
     CDS             35256..35447
                     /locus_tag="ROI21_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDX5109630.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(35448..36104)
                     /gene="pphA"
                     /locus_tag="ROI21_000666"
     CDS             complement(35448..36104)
                     /gene="pphA"
                     /locus_tag="ROI21_000666"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDX5109631.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALTSQQMSLWLMNGGD
                     WFIALADNQQKQAKTTLEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(36500..36841)
                     /locus_tag="ROI21_000667"
     CDS             complement(36500..36841)
                     /locus_tag="ROI21_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDX5109632.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     TLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(36854..37726)
                     /gene="copD"
                     /locus_tag="ROI21_000668"
     CDS             complement(36854..37726)
                     /gene="copD"
                     /locus_tag="ROI21_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDX5109633.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAT
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(37730..38104)
                     /gene="yobA"
                     /locus_tag="ROI21_000669"
     CDS             complement(37730..38104)
                     /gene="yobA"
                     /locus_tag="ROI21_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDX5109634.1"
                     /translation="MTSTARTLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            38243..38473
                     /gene="holE"
                     /locus_tag="ROI21_000670"
     CDS             38243..38473
                     /gene="holE"
                     /locus_tag="ROI21_000670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDX5109635.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            38575..39231
                     /locus_tag="ROI21_000671"
     CDS             38575..39231
                     /locus_tag="ROI21_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDX5109636.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            39255..39917
                     /gene="exoX"
                     /locus_tag="ROI21_000672"
     CDS             39255..39917
                     /gene="exoX"
                     /locus_tag="ROI21_000672"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDX5109637.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(39914..41974)
                     /gene="ptrB"
                     /locus_tag="ROI21_000673"
     CDS             complement(39914..41974)
                     /gene="ptrB"
                     /locus_tag="ROI21_000673"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDX5109638.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRRGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(42183..42842)
                     /locus_tag="ROI21_000674"
     CDS             complement(42183..42842)
                     /locus_tag="ROI21_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDX5109639.1"
                     /translation="MANWLNQLQSLLGQSSSSASSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     IRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(43169..43525)
                     /locus_tag="ROI21_000675"
     CDS             complement(43169..43525)
                     /locus_tag="ROI21_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDX5109640.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(43592..43882)
                     /gene="yebG"
                     /locus_tag="ROI21_000676"
     CDS             complement(43592..43882)
                     /gene="yebG"
                     /locus_tag="ROI21_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDX5109641.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            44016..45194
                     /gene="purT"
                     /locus_tag="ROI21_000677"
     CDS             44016..45194
                     /gene="purT"
                     /locus_tag="ROI21_000677"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDX5109642.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFREAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSEN
                     VTFDNVQNAVGAGLQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAAQIKVQA
                     "
     gene            complement(45249..45890)
                     /gene="kdgA"
                     /locus_tag="ROI21_000678"
                     /gene_synonym="eda"
     CDS             complement(45249..45890)
                     /gene="kdgA"
                     /locus_tag="ROI21_000678"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDX5109643.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(45927..47738)
                     /gene="edd"
                     /locus_tag="ROI21_000679"
     CDS             complement(45927..47738)
                     /gene="edd"
                     /locus_tag="ROI21_000679"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDX5109644.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(47973..49448)
                     /gene="zwf"
                     /locus_tag="ROI21_000680"
     CDS             complement(47973..49448)
                     /gene="zwf"
                     /locus_tag="ROI21_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDX5109645.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            49786..50655
                     /gene="hexR"
                     /locus_tag="ROI21_000681"
                     /gene_synonym="yebK"
     CDS             49786..50655
                     /gene="hexR"
                     /locus_tag="ROI21_000681"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDX5109646.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            50783..52225
                     /gene="pyk"
                     /locus_tag="ROI21_000682"
     CDS             50783..52225
                     /gene="pyk"
                     /locus_tag="ROI21_000682"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDX5109647.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(52357..53328)
                     /gene="lpxM"
                     /locus_tag="ROI21_000683"
                     /gene_synonym="msbB"
     CDS             complement(52357..53328)
                     /gene="lpxM"
                     /locus_tag="ROI21_000683"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDX5109648.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(53447..54769)
                     /gene="mepM"
                     /locus_tag="ROI21_000684"
     CDS             complement(53447..54769)
                     /gene="mepM"
                     /locus_tag="ROI21_000684"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDX5109649.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(54785..55717)
                     /gene="znuA"
                     /locus_tag="ROI21_000685"
     CDS             complement(54785..55717)
                     /gene="znuA"
                     /locus_tag="ROI21_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDX5109650.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLENVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTEKQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            55796..56551
                     /gene="znuC"
                     /locus_tag="ROI21_000686"
     CDS             55796..56551
                     /gene="znuC"
                     /locus_tag="ROI21_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDX5109651.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPDTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            56548..57333
                     /gene="znuB"
                     /locus_tag="ROI21_000687"
     CDS             56548..57333
                     /gene="znuB"
                     /locus_tag="ROI21_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDX5109652.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVLAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(57483..58493)
                     /gene="ruvB"
                     /locus_tag="ROI21_000688"
     CDS             complement(57483..58493)
                     /gene="ruvB"
                     /locus_tag="ROI21_000688"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDX5109653.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(58502..59113)
                     /gene="ruvA"
                     /locus_tag="ROI21_000689"
     CDS             complement(58502..59113)
                     /gene="ruvA"
                     /locus_tag="ROI21_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDX5109654.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(59252..59317)
                     /gene="yobI"
                     /locus_tag="ROI21_000690"
     CDS             complement(59252..59317)
                     /gene="yobI"
                     /locus_tag="ROI21_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HDX5109655.1"
                     /translation="MCIFITHFFTEYVIFKYLLPI"
     gene            59388..59990
                     /locus_tag="ROI21_000691"
     CDS             59388..59990
                     /locus_tag="ROI21_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDX5109656.1"
                     /translation="MNINYPAEYEIGDIVFTCIGTALFGQISAASNCWSNHVGIITGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(59992..60513)
                     /gene="ruvC"
                     /locus_tag="ROI21_000692"
     CDS             complement(59992..60513)
                     /gene="ruvC"
                     /locus_tag="ROI21_000692"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDX5109657.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(60548..61288)
                     /locus_tag="ROI21_000693"
     CDS             complement(60548..61288)
                     /locus_tag="ROI21_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDX5109658.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(61317..61769)
                     /gene="nudB"
                     /locus_tag="ROI21_000694"
     CDS             complement(61317..61769)
                     /gene="nudB"
                     /locus_tag="ROI21_000694"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDX5109659.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(61887..63659)
                     /gene="aspS"
                     /locus_tag="ROI21_000695"
     CDS             complement(61887..63659)
                     /gene="aspS"
                     /locus_tag="ROI21_000695"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDX5109660.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            63969..64535
                     /locus_tag="ROI21_000696"
     CDS             63969..64535
                     /locus_tag="ROI21_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDX5109661.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDVPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            64532..65350
                     /locus_tag="ROI21_000697"
     CDS             64532..65350
                     /locus_tag="ROI21_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDX5109662.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKS
                     EVVLRWREQTTDNFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRDLPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            65403..65798
                     /locus_tag="ROI21_000698"
     CDS             65403..65798
                     /locus_tag="ROI21_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDX5109663.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            65839..66582
                     /gene="cmoA"
                     /locus_tag="ROI21_000699"
     CDS             65839..66582
                     /gene="cmoA"
                     /locus_tag="ROI21_000699"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDX5109664.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HQAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            66579..67550
                     /gene="cmoB"
                     /locus_tag="ROI21_000700"
     CDS             66579..67550
                     /gene="cmoB"
                     /locus_tag="ROI21_000700"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDX5109665.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(67585..70014)
                     /gene="torZ"
                     /locus_tag="ROI21_000701"
     CDS             complement(67585..70014)
                     /gene="torZ"
                     /locus_tag="ROI21_000701"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDX5109666.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IIKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAAQFEARNDFDVFADLAELLKPGGKEIYTEGRDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNRRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(70039..71139)
                     /locus_tag="ROI21_000702"
     CDS             complement(70039..71139)
                     /locus_tag="ROI21_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDX5109667.1"
                     /translation="MRGKKRIGLMFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGIDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKPSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVDGEWQADE
                     YGNQWRQASLQGVLTDPVLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT        17154 a        18498 c        17541 g        18231 t
ORIGIN      
        1 tactgcggat attcctgcaa taaattgtgt aatgcttccg ccatcagctc accacgccat
       61 ccggaaatca gctccggcaa attgttctgc ggtttcagtt tccagtgcca gttcagcagc
      121 tggttgattt gccgacgcga tgccagcaat tcggcgctga tcttgtgcgt ttcgctcacg
      181 tcagtaatca acgacttaat cgctttaaat gctttacgat aacccggcat gtccatcagg
      241 ttaagcatcg gctgcggtaa ggcctcttcc ggcaatgcct gcgctttttc caccagcgcc
      301 agcagcgttt taccgtgaaa gcggatttcg ctaccggata agcccaggct gtccagttcg
      361 cctaaacttc ccggcatata acgcgctaca gaccacaaat gctcttcacg cacgacaaag
      421 ttcactgcca gatcgcgctc ccgagccttg cgcagtcgcc agtcggctaa cagttgcaga
      481 caggccagtt ggcgtgtgcg taactgccag gcattggtga tatcacgcca ggcatcttcc
      541 ggtgcaacga cttcctgacg gcgcatttgc atcaggcggc attcatccag cgccgcaggt
      601 agccagccgg aggcctccgt ttctaccata agctttgcgg tgatcggtaa cagataccag
      661 acatccgccg ctgcgtattc acactgacgt tcggtcagcg gtctggccag ccagtcggtg
      721 cgcgattcac tcttatccag cgtaacgccg gaatactctt ccaccatgga agcgaaaccc
      781 catgacatcg ggcgtccgca gaaggcagca aggatttgcg tgtcaatcaa gggttgtggt
      841 aattcgccaa agacattgag gaacacttcc agatcttcac tgcccgcatg gagaaatttt
      901 gtgatggacg gatcgcgcag gatcgctttc agcggtgacc agtcggagat cccgagagga
      961 tcaattagcg ccagatgctc gccatcgaaa agttgaatca accccagctg cgggtaataa
     1021 gtgcgcgtac gaacaaattc agtatccagg gctatcgccg gaaaggcacg gacggcttca
     1081 cacaaagaag ccagcgcatc gtccgtggta atcatttggt aattcaaatt gtattctctt
     1141 tagtgggcgt caaaaaaaac gccggattaa ccggcgtctg acgactgact taacgctcag
     1201 gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt ctcgtcgcaa aatttttccg
     1261 acgttagatt tcggtaactc atcacgaaac tccaccagct tcggtacttt gtagcccgtg
     1321 agctgacggc ggcaaaaagt caccagtgac tcttcggtaa gcgatggatc ttttttcact
     1381 acgaagattt tcaccgcttc accactggag ccggaaggta cgccaacagc cgcgacttcc
     1441 tgtacgccag gatgctgcat gacgacatct tcaatctcgt tgggatagac gttaaaaccg
     1501 gaaaccagaa tcatgtcttt tttacgatcg acaatgcgca ggaatccttc ttcatccatc
     1561 accgcgatgt cgccggtgtg taaccagcca tttttgatga tttcatcggt agcatccgga
     1621 cgctgccagt aacccagcat cacctgcggt cctttgacac aaagctcacc cggttgaccc
     1681 ggtggtactt cgttgtcgtc gtcatccacc agtttggctt ccgtcgatgg caccggcaaa
     1741 ccgatgctac cactatgata atcaatatca tacgggttaa cgctgaccag cggcgcacac
     1801 tcggtcaggc cataaccttc cagcagatac tgccctgtca gtttcaccca acgttctgcc
     1861 accacttgct gaacaggcat cccaccgcct gcggaaaggt gcagactgga gaaatccagc
     1921 tgctggaact ctttattgtt cagcaacgca ttgaataagg tgttaacgcc agtgatagcg
     1981 gtaaacggat atttcgctaa ctcttttacc aaccctggaa tatcgcgcgg gttagtgata
     2041 agcaggttct gcccacccag ttcgataaac agcaggcagt taatggtcag tgcaaaaatg
     2101 tgataaagcg gcagcgccgt caccaccagc tctttgcccg gatgcaacag cggaccatag
     2161 gtcgcgttaa cctgttccag gttcgccagc atattgcggt gagtcagcat cgcgcctttc
     2221 gccacaccag tggtaccgcc ggtgtattgc aggaaagcta aatcttccgg caccagttcg
     2281 ggtttgacgt actgcatccg gtagccgttg tgcagggcgc tacgaaatga aatggcatct
     2341 ggcagatggt atttcggcac cagacgcttg atgtatttaa caacgaaatt gactaccgtg
     2401 ccttttgccg tagatagctg atcgcccata cgggtcagaa ttacgtgctg aacggcggtt
     2461 ttatcaacca ctttttccag agtgtgggca aagttagaca cgataacaat cgccgatgcg
     2521 ccgctatcgt taagctgatg ctcaagttca cgcggggtat acaacgggtt aacgtttacg
     2581 acaatcatcc cggcacgcaa aatgccaaac agcgccaccg gatattgcaa taaattaggc
     2641 atcatcaacg caacgcgatc gcctttcttc agtcccaacc cttgttgcaa ataagcggca
     2701 aacgcgcgac tgcgttcttc cagcttgcgg aaggtcatca cctcccccat attcacaaac
     2761 gcaggctgat cggcgtagcg cgcgaccgac tgctcaaaca tatctaccag agattgataa
     2821 cggtcagggt tgatctccgt cggaacgtcc gcgggataac ggttaagcca aaccttcttc
     2881 aattcttcac ctctaaaatg cgtgttcgtc gtcatcgcaa ccccaaatga tacacatgcc
     2941 gttaacataa tattaactca tcataccagc ttgataatta cccaacgaaa aggttgcgaa
     3001 gcgcgtcact atttattttt atctttaccg taagaatgca gaaacagcgg accagccgct
     3061 gtttcttttt ctttaaaaac aagcaattat tcagttacaa ctgtttgtac tctcgcgggg
     3121 ccgggattat accagcccca tccgccgtag ccatagggcc agccacgacc gccataaaac
     3181 catggatcaa tcggctgagg cggcataatc acctgctggg ttaaatgcca acgtttgtaa
     3241 cccgttacct gcatcaccat aaatttatag ggcgtgttgc cgattttgcc gtcaaccgca
     3301 ccggtgattg gcccaactac cgtaaccagt tgtccacgga aatcgaccgg gtccagaaaa
     3361 ccgttcacat cagcataaat gcgaccgcga gaaggttctc ccagcgtcgg tctggcaccg
     3421 ctgtccagcg gtacggtagc aatttccagg cgggttttcc cttgctggtt ttgtaccgca
     3481 accactttgc cgccaaagcg agcctcctga ccaacgtaca gctgcggcgc actcatcacc
     3541 cgaactaaat cttgttgcgg cgtgcgactg ctgcctttaa tggcgtccgg cacagtgaca
     3601 caaccgctga gcattagcgc aaacgtacct gccagtatgc ctttgataac attcttttga
     3661 accgccatga tgcgactcct tttttctcag ggcatactct taagattcat tctttgcccg
     3721 gaagtttctt ccatgcgacg ttgttacgta aataaaccgg ttcggcatgt tccaccgcca
     3781 ccgttttact ctcggcaaac atctgacaag caatcggcag catatcttct gcagcgggta
     3841 gtaacacttc gccatcgcac agggccaaac cgctctcttt accgagatcc ggccaggctt
     3901 gccagcccgt ccctaccgtc acccattcgc cggaaagctg ttgcatccgt tcatgaacga
     3961 gttcaggttt cagtacggct tcggtttctt caccgtgcca gataccgttt tcatcacgct
     4021 gatattcggc ccagtaaact tcgcccattc gcgcgtcaat ggctgccagc acgcgtgttg
     4081 cgccgttttt gcgccacgcc ccttgcgcca tcgtcattag cgtggagacg ccaatcatcg
     4141 gtaattccgc gccaagcgcc agcccttgcg cgatgccaat accaatgcgc acgccggtaa
     4201 agctaccggg gccgcgcccg taagccagag catttatgtc cgtcagggaa gttccactgg
     4261 tggtcaggat atcctgcacc atcggtaaga ttcgttgagt atgttcacga gggcaaagct
     4321 caaaatgagc gttgacagta ccgtcgttcc acagggcgac agagcaggcc tctgtcgcgg
     4381 tatcgatagc cagaattcgc atgggtcttc gtgcttagat caataaaaag gggcgcatca
     4441 taccatactc cgtaacaaat tacctggagg atggtatcgc aaggaaacga accgcacggg
     4501 caatgtcacg ggtgcgcggc gcgggcggca gactggcgag aaacgtcgcg ccgtaagggc
     4561 gcatcaccag ccgattatcg caaatcacca gcacgccgcg atcgtcagcg tcgcgaatca
     4621 gtcgccctac tccctgcttg agagtaatca ccgcatccgg cagttgtact tcatcgaacg
     4681 ggtcaccacc acgcaaacgg caatcttcca tgcgcgcttt taacaatgga tcatccggcg
     4741 aggtaaacgg caatttgtcg ataataacca atgacaatgt atcgccacgc acatccaccc
     4801 cctcccagaa actgctggtt gccacaagaa gcgcgttacc ggcactgaca aattgctgca
     4861 acaattgccc tttgctggtt tccccctgca ataatacggg aagcgtcatg gtagcgcgga
     4921 actgctcggc aagatcgcgc atcatggcgt gcgaggtaca aagcataaaa caacgaccgt
     4981 tgttagcttc gatgatcggt cgcaacattg ccgccagttg ccgtgcggag cccggttgat
     5041 tggtttgcgg caggttgcgc ggcacacaga gtaacgcctg gcggctgtaa tcaaaggggc
     5101 tgggtaacag caacgactct gcctgttcaa tgccaagccg cgaggtgaaa tgatgcagat
     5161 cgtcgttcac cgacagcgtt gctgaggtaa agatccagct accgggtttt tgcgccatta
     5221 actctttgaa tttatccgcc acgctgagcg gcgtgagtgc cagagtaaaa tggcgcgaag
     5281 tgcattcgta ccagtagctg tagcccggct gattgatctc ttttagccgt ttcagccgcg
     5341 tacgatacaa cgtggcgcgc tcaaatgccg catccagcaa ggcggaacgc cccagcgaca
     5401 gtttcgccac gtcataacaa agttccaggg tgtcatcgag cagtaaaaac gcccgttgaa
     5461 tttgcggatt agctagcagt tcgcgcaggt tgccacgata accaggctca ccgagttgca
     5521 gacgaaaatc ctgcgcgctc tgggcaaggc ggtcggcgca cttttgtaac tgctgagtat
     5581 cttttaattc ggtgcgatag gcgatggtga tgtcttttgc caggtcgagc agttgtctac
     5641 tggagagtga ctgaccaaaa tactggctgg caatgtcggg cagttggtgg gcttcgtcga
     5701 agatcatgac gtcagcttcc gggatcagtt cgccaaatcc actctctttc accaccatat
     5761 ccgccagaaa gagatgatgg tttaccacca ccacatcggc gtccatcgct tttttgcgtg
     5821 ctttgaccac aaagcaatct ttatacatcg ggcagtcgct gccaaggcag ttatcgttgg
     5881 tgctggtgac cagcggccac gcctgtgaat cttccgccac gctgacgcag gtgctgatat
     5941 caccatcgac tgtttgatta gaccaggagc gcagcaggat cacatcgctt aagatttgta
     6001 ccggcagatc gccccccgcc agcgcctgct gttcgagacg ttcgaggcag aggtagtttg
     6061 agcgcccttt cagcaacgcc acgttgcccg tatatttcaa cgcctttgaa accgttggca
     6121 aatcgcggct gtagagctga tcctgcaacg cttttgaacc ggtcgagata atgactttct
     6181 ttttcgcccg cagcgcaggg gccaggtaag cgtaggtttt gcccgtaccg gttccggctt
     6241 ccaccaccag cggctggcct ttttctatcg cctgggtgac ggctaccgcc atctgtcgct
     6301 gtggttctcg cggcttaaag cccggtatcg ctttcgccaa ctgaccgtct ggtgcaaaat
     6361 cgtccgtcac actaccccct gttgatttga acagggatta tgtcaggatg agggcgcttt
     6421 cgccagttga agtggtgacg gcgacctcac attgtggcag tctttgcagc acgaaaatgg
     6481 aaggataatg aggacaaaat gatgactatc gttcgtatcg atgctgaagc ccgctggtct
     6541 gatgtagtaa tccacaacaa cacgctctac tacactggtg taccggaaaa cctcgacgcc
     6601 gatgcctttg agcaaaccgc caacacgctg gcgcagattg acaccgtgct ggaaaaacag
     6661 ggcagcaata aatcgagcat tctggatgcc accattttcc tggccgataa aaacgacttc
     6721 gcggcgatga ataaagcgtg ggatgcctgg gttgtcgcgg gtcatgcgcc ggtgcgctgc
     6781 acggtacaag cgggtttgat gaacccgaag tataaagttg aaattaagat tgtggctgca
     6841 gtgtaagctt tatcgaagca taataagtca gacgataatt tatcgataat actggtcggt
     6901 tttgcataaa tcgaccagag attaagttat tactgttgat gctcctgcta attcattatt
     6961 tatttttata atttcaattt catctataaa actagctata taaataaaca taaccatgcc
     7021 gttgtgttcg ccctttgatt agcaactctg gttttctttc ttagctgaac aatctggtta
     7081 tagcgtgcgg actgaccgtt aattttctcc ctcttactgg ataacacacc atgagctgct
     7141 acctttaaaa cacctgaatg tcaacaggtt aactcgtgcc atatcgttat aatccctttg
     7201 atattagatg caaattaagg tcatatatag ccttatcgat aacatggtta attttaagga
     7261 taagaatatg cctgctgtaa tagataaagc cctggatttc attggtgcca tggatgtatc
     7321 agcaccaaca ccaagttcga tgaatgaaag cacggcgaag ggaatcttta aatatttaaa
     7381 agaactggga gtacccgcga gcgccgccga tattacggcg cgagctgacc aggaaggctg
     7441 gaacccgggg ttcacggaaa aaatggttgg atgggcaaaa aaaatggagt ctggtgaacg
     7501 cattgtgatt aaaaatcctg aatacttttc aacatatatg caggaagaac taaaagcact
     7561 ggtctgagtt aaatttacat cagcataaat gggtcaggac gcttttaatc acatatgaaa
     7621 agcgtccttt ttctcacaat caaataatca tcggcttgta taacgaaagc gatacggatt
     7681 agtgtgttac tcgtcttcat cctcaaaacg cgccacgatt cgctcgccgg agtggttggc
     7741 acgcagctct tctgccacca gcgcgattgc ctggccgctg ctcatccctt gtgccatcag
     7801 ttcctggatc cgctcgacag ctttttgctg ctgttcatgg gtgagtgaag gtaaacctgc
     7861 aaacattgtt aactcctgct aaattgttgg cgctaattat ttcatgctac ccggcacata
     7921 gccagtagag tcaggactga tgaagacgtt atctcccgct gtgattactt taccctggcg
     7981 tcaggacgcc gctgaatttt atttctcccg cttaagccac ctgccgtggg cgatgctttt
     8041 acactccggc tatgccgatc atccgtatag ccgctttgat attgtggtcg ccgagccgat
     8101 ttgcacttta accactttcg gtaaagaaac cgttgttagt gaaaacgaaa aacgcacaac
     8161 gaccactgat gacccgctac aggtgctcca gcaggtgctg gatcgcgcag acattcgccc
     8221 agcgcataac gaagatttgc catttcaggg cggcgcgctg gggttgtttg gctacgatct
     8281 gggccgccgt tttgagtcac tgccagaaat tgcgcagcaa gatatcgttc tgcctgatat
     8341 ggcagtgggt atctacgact gggcgctggt tgttgaccac cagcgtcaaa cagtttcttt
     8401 gctgagtcat aatgatgtca atgcccgtcg ggcctggctg gaaagccagc aattctcccc
     8461 gcaggaagat ttcgcgctca cttccgactg gcaatccaat atgacccgcg agcagtacgg
     8521 cgaaaaattt cgccaggtac aggaatatct gcacagtggt gattgctatc aggtgaatct
     8581 cgcccagcgt tttcaagcgg cctattctgg cgatgaatgg caggcattcc ttcagcttaa
     8641 tcaggccaac cgcgcgccgt ttagcgcttt tttacgcctt gaacagggcg cgattttaag
     8701 cctttcgcca gagcggttta tcctttgtga taatagtgaa atccagaccc gcccgattaa
     8761 aggcacgcta ccacgcctgc ccgatcctca ggaagatagc aaacaagcag agaaactggc
     8821 gaactccccc aaagatcgcg ctgaaaatct gatgattgtc gatttaatgc gtaatgatat
     8881 cggtcgtgtt gccgtagcag gttcggtaaa agtaccagag ctcttcgtgg tggaaccctt
     8941 ccctgccgtg catcatctgg tcagcaccat aacggcgcaa ctaccagaac agttacacgc
     9001 cagcgatctg ctgcgcgcag cttttcctgg tggctcaata accggggctc cgaaagtacg
     9061 ggctatggaa attatcgacg aactggaacc gcagcgacgt aatgcctggt gcggcagcat
     9121 tggctatttg agcttttgcg gcaacatgga caccagcatt actatccgca cgctgactgc
     9181 cattaacgga caaatttact gctctgcggg cggtggaatt gtcgccgata gccaggaaga
     9241 agcggaatat caggaaactt ttgataaagt taataagata ttacgccaac tggagaagta
     9301 agacgtggaa taccgtagcc tgacgcttga tgatttttta tcgcgctttc aacttttgcg
     9361 cccgcaaatt aaccgggaaa ccctaaatca tcgtcaggct gccgtgttaa tccccatcgt
     9421 ccgtcgaccg caaccggggt tgttgctgac tcagcgttcg attcatctgc gtaaacacgc
     9481 tggacaagtg gcattccctg gaggcgcagt cgatgacacg gacgcatcag ttatcgccgc
     9541 tgcgctgcgc gaagctgaag aagaggtcgc tataccgcct tccgccgttg aagttatcgg
     9601 cgtgctgccg cccgtcgata gcgtaactgg ctatcaggta accccggtgg tcggcattat
     9661 cccgcccgat ctgccgtatc gcgccagtga agatgaagtc tcggcggtgt ttgaaatgcc
     9721 gctcgcccag gcattacatc tgggtcgtta tcacccttta gatatctacc gccgtggcga
     9781 ttcacatcgg gtatggctgt cctggtacga acagtatttt gtatggggaa tgaccgcagg
     9841 cattattcgt gaactggcgc tgcaaattgg tgtgaaaccc tgactatact tatctttaca
     9901 tctacaaaac actacttgag acaatcatcg caatattagt taaatcgcgg tttttgatta
     9961 gtttaattca tgtgaatagt taagccagtc gccgcgttcc ctcttacact atgcgctgtt
    10021 attagttcgt tactggaagt ccagtcacct tgtcaggagt attatcgtga ttagtctatt
    10081 cgacatgttt aaggtgggga ttggtccctc atcttcccat accgtagggc ctatgaaggc
    10141 gggtaaacag ttcgtcgatg atctggtcga aaaaggctta ctggatagcg ttactcgcgt
    10201 tgccgtggac gtttatggtt cactgtcgct gacgggtaaa ggccaccaca ccgatatcgc
    10261 cattattatg ggtcttgcag gtaacgaacc tgccaccgtg gatatcgaca gtattcccgg
    10321 ttttattcgc gacgtagaag agcgcgaacg tctgctgctg gcacagggac ggcatgaagt
    10381 ggatttcccg cgcgacaacg ggatgcgttt tcataacggc aacctgccgc tgcatgaaaa
    10441 cggtatgcaa atccacgcct ataacggcga tgaagtcatc tacagcaaaa cttattattc
    10501 catcggcggc ggttttatcg tcgatgaaga acactttggt caggatgctg ccaacgaagt
    10561 gagcgtgccg tatccgttca aatctgccac cgaactgctc gcgtactgta atgaaaccgg
    10621 ctattcgctg tctggtctcg ctatgcagaa cgaactggcg ctgcacagca agaaagagat
    10681 cgacgagtat ttcgcgcatg tctggcaaac catgcaggca tgtatcgatc gcgggatgaa
    10741 caccgaaggt gtactgccag gcccgctgcg cgtgccacgt cgtgcgtctg ccctgcgccg
    10801 gatgctggtt tccagcgata aactgtctaa cgatccgatg aatgtcattg actgggtaaa
    10861 catgtttgcg ctggcagtta acgaagaaaa cgccgccggt ggtcgtgtgg taactgcgcc
    10921 aaccaacggt gcctgcggta tcgttccggc agtgctggct tactatgacc actttattga
    10981 atcggtcagc ccggacatct atacccgtta ctttatggca gcgggcgcga ttggtgcact
    11041 gtataaaatg aacgcctcta tttccggtgc ggaagtcggt tgccagggcg aagtgggtgt
    11101 tgcctgttca atggctgctg cgggccttgc cgagctgttg ggcggtagcc cggaacaggt
    11161 ttgtgtggca gcggaaattg gcatggagca caacctcggt ctgacctgtg accctgttgc
    11221 agggcaagtt caggtgccgt gcattgaacg taacgccatt gcgtcagtca aggcgattaa
    11281 cgccgcgcgg atggccctgc gtcgcaccag tgcaccgcgc gtctcgctgg ataaggtcat
    11341 cgaaacgatg tacgaaaccg gtaaggacat gaacgctaaa taccgcgaaa cctcacgcgg
    11401 tggtctggca atcaaagtcc agtgtgacta atacttctta ctcgcccatc tgcaacggat
    11461 gggcgaattt atccccgctt tctcgtctgc tgtaatattc cccactacac ttccactgtt
    11521 gcgtcaggcg tttgtcgcca tacgcttaca gggtggcccg catgcaaaaa gcacaacgga
    11581 tcattaaaac ctatcgccgt aatcgaatga ttgtttgtac gatttgcgca ctcgttacgc
    11641 tcgcttcgac cctgagcgtg cgatttattt cacagcgtaa cttaaatcaa caacgggtag
    11701 tacaattcgc caatcacgct gtagaggaat tagataaagt actgcttccc ctacaggcag
    11761 gtagcgaagt cttgcttccg ctgattggtc tgccctgctc tgtcgcccat ttgccattac
    11821 gtaaacaggc ggcaaaactc caaactgtgc gatccattgg cctggtgcaa gacggcacac
    11881 tttattgctc cagcattttt ggttatcgca atgtgcccgt cgtggacatt ctggctgaac
    11941 ttcctgcacc gcaaccactt ttacgcctga cgatcgaccg tgccctgatt aaaggcagtc
    12001 cggttttgat tcaatggaca ccttcagcgg gcagtagcaa tgctggggtc atggagatga
    12061 ttaacatcga cttactgacg gcaatgctcc ttgagccaca actgccgcaa atcagtagcg
    12121 ccagcctgac ggtgggcaaa cggcatttgc tctatggtaa tgggctggta gattcccttc
    12181 cgcaacctga agacaatgaa aactatcagg tttcttcgca acgctttcct tttaccatta
    12241 acgttaatgg tccgggggct acggcgctgg catggcacta tcttccaaca caattaccgc
    12301 tggcggtgct gctaagttta ctggtgggct acatcgcctg gctggcgacc gcttaccgaa
    12361 tgagcttttc ccgcgagatc aatctgggcc tggcgcaaca tgagttcgaa ttgttctgcc
    12421 agcctttgct taatgcgcgc agccagcaat gtattggtgt agagattttg ctgcgctgga
    12481 acaatccacg tcagggctgg atttcaccgg atgtgtttat tcctatcgcg gaagaacatc
    12541 atttaattgt gccactgacc cgctatgtga tggcagaaac catccgtcag cggcatgttt
    12601 tcccaatgag tagtcagttt catgttggca ttaacgtcgc acccagccat tttcgccgtg
    12661 gtgtgctgat aaaagatctc aatcagtact ggtttagcgc tcacccgatt cagcaactga
    12721 tcctcgaaat caccgaacgc gatgccttac tggatgtgga ttatcggatt gcccgcgagc
    12781 tgcatcgtaa aaacgtcaaa ctggcgattg atgacttcgg caccggcaac agctcgtttt
    12841 cctggcttga aacattacgt cctgacgtgc tgaaaattga taagtcattt accgcagcta
    12901 taggttctga cgcggttaac tcgacggtga ccgatatcat catcgcactg gggcaaagac
    12961 tgaatattga actggtggcg gagggtgtgg aaacgcaaga acaggcgaaa tatttgcgcc
    13021 gtcatggcgt ccatattttg caagggtatt tgtacgcaca gccgatgcca ctatgtgatt
    13081 ttcccaaatg gctggcgggc agccaaccgc cgcccgcccg gcataatgga catatcacac
    13141 ccattatgcc gttacgttaa cgcttactca tcttcatcgt gcgccggttg ctctttaaca
    13201 atgcgaacca gatcaacacg ataatcattg gcttcaatga tggtgatatg cagtggccct
    13261 acatcaatca catcgcccac acggggaatg tgaccatttg ccgagatcac gaggcccgcg
    13321 accgtcgcga tatcatcgtc atcggcaagg tgctcaacat caagcgcctg ctgcaaggca
    13381 tgcaaatctg taccgccttt taccagccag ccgtcaccat cggtaatgat ttccggcgtt
    13441 tcgtcagcgt ccgggaattc acccgcaatg gcttccagca catccagcgg cgtgaccaga
    13501 ccttgtacca caccaaactc gttggtcacg ataacaaagc tcccgcgagc acgacgcagc
    13561 acgcccagca ggttgatcgg atcgagggtt tcagggacga taatcgccgg agacgccgaa
    13621 gcaatcgccg ccacatcaac gccctcttcc agcgccacca gcagttcttt agcacgtacg
    13681 attccgatga tttcatccag ttcaccgcga cataccggga acaggctgtg cggtgaggag
    13741 agcagctgct cgcggatttc atcgaccccg agattagcgt caacccagct tatttcaccg
    13801 cgcggcgtca tgatcccgcg cagagaacgc gacgccagcg tgagtacgcc gttaatcatg
    13861 taacgttctt cttcggcaaa cgcaccttcc gggatcggca tcggcatcgg gttatcggca
    13921 tcgtgctgaa cattggcctg acgtttcccg cccatcaaac gcaggatggc atcggcagta
    13981 cgcgctcgca gcggcaaagt cgactggtga cgaataaagt tgcgacgcgc aatctggtta
    14041 aacacttcga tgatgatcga gaagccaatc gcggcataca ggtaaccttt cggaatgtgg
    14101 aaaccgaaac cttctgccac cagactcaga cctatcatta acaggaagct cagacagagc
    14161 accaccaccg tgggatgctg gttaacaaat cgcgtcagcg gtttcgatgc cagcagcata
    14221 accgccatcg caatcactac cgccgccatc atcactggca gatggttaac catccctact
    14281 gccgtaatta ccgcatccaa tgagaagacg gcgtcaagga tgacaatctg tgtgacgacc
    14341 acccagaaac tggcgtagcc tttaccgtgg ccggaatcat gatcgcggtt ttccagccgt
    14401 tcatgcagtt cggttgttgc tttgaacagc aagaatatcc ccccgaacaa cataatcagg
    14461 tcgcgtccgg agaaggagaa atccatgacg gtaaatagcg gtttggtcag cgtgaccatc
    14521 catgaaatca gcgacagcag ccccagacgc ataatcagcg ccagtgataa tcccagcaaa
    14581 cgcgctttat cgcgttgttt tggcggcagt ttgtcagcaa gaatggcgat gaagaccagg
    14641 ttatcgatac ccagcacaat ttcgagaaca acaagcgtga gtagccccgc ccaaattgag
    14701 gggtccatta agaattccat gacaagctcc tgcttaagga atagctattc gacgccagaa
    14761 ataatgcagg cgtaacgaca aaatgcaaac gaaaggtgcg gcatagagtg ccagaaaggc
    14821 aggcgttaaa aggcctgatg ctgaaatgac gtcggtgacg atccatactg cgggctactg
    14881 ccctatactc catggttgtt aaacgggagt taaacatatc agagacgcct ctgatttggc
    14941 aaagatttac cttcctttgc aaacgaatgt gacaaggata ttttaccttt cgaaatttct
    15001 gctaatcgaa agttaaatta cggatcttca tcacataaaa taattttttc gatatctaaa
    15061 ataaatcgcg aaacgcaggg gtttttggtt gtagccctta tctgaatcga ttcgattgtg
    15121 gacgacgatt caaaaataca tctggcacgt tgaggtgtta acgataataa aggaggtagc
    15181 aagtgaccat tgctattgtt ataggcacac atggttgggc tgcagagcag ttgcttaaaa
    15241 ccgcagaaat gctgttaggc gagcaggaaa acgtcggctg gatcgatttc gttccaggtg
    15301 aaaatgccga aacgctgatt gaaaagtaca acgctcagtt ggcaaaactc gacaccacta
    15361 aaggcgtgct gtttctcgtt gatacatggg gaggcagccc gttcaatgct gccagccgca
    15421 ttgtcgtcga caaagagcat tatgaagtca ttgcaggcgt taacattcca atgctcgtgg
    15481 aaacgttaat ggcccgtgat gatgacccaa gctttgatga actggttgcg ctggcagtag
    15541 aaacaggccg tgaaggcgtg aaagctctga aagccaaacc ggttgaaaaa gccgcgccag
    15601 cacccgctgc cgcagcacca aaagcggctc caactccggc aaaaccaatg ggaccaaacg
    15661 actacatggt tattggcctt gcgcgtatcg acgaccgtct gattcacggt caggtcgcca
    15721 cccgctggac caaagaaacc aatgtctccc gtattattgt tgttagtgat gaagtggctg
    15781 cggataccgt tcgtaagaca ctgctcaccc aggttgcacc tccgggcgta acagcacacg
    15841 tagttgatgt tgccaaaatg attcgcgtct acaacaaccc gaaatatgct ggcgaacgtg
    15901 taatgctgtt atttaccaac ccaacagatg tagagcgtct cgttgaaggc ggcgtgaaaa
    15961 tcacctctgt taacgtcggt ggtatggcat tccgtcaggg taaaacccag gtgaataacg
    16021 cggtttcggt tgatgaaaaa gatatcgagg cgttcaagaa actgaatgcg cgcggtattg
    16081 agctggaagt ccgtaaggtt tccaccgatc cgaaactgaa aatgatggat ctgatcagca
    16141 aaatcgataa gtaacgtatt gtgttgatta tcactcagtt ttcacacttt agtcttacgt
    16201 aaacaggaga agtacaatgg agattaccac tcttcaaatt gtgctggtat ttatcgtagc
    16261 ctgtatcgca ggtatgggat caatcctcga tgaatttcag tttcaccgtc cgctaatcgc
    16321 gtgtaccctg gtgggtatcg ttcttggcga tatgaaaacc ggtattatta tcggtggtac
    16381 gctggaaatg atcgcgctgg gctggatgaa catcggtgct gcagttgcgc ctgacgccgc
    16441 tctggcttcc atcatttcta ccattctggt tatcgcaggt catcagagca ttggtgcagg
    16501 tattgcactg gcaatccctc tggccgctgc gggccaggta ctgaccatca tcgttcgtac
    16561 tattaccgtt gctttccagc acgctgcgga taaggctgct gataacggca acctgacagc
    16621 gatttcctgg atccacgttt cttctctgtt cctgcaagca atgcgtgtgg ctattccggc
    16681 cgtcatcgtt gcgctgtctg ttggtaccag cgaagtacag aacatgctga acgcgattcc
    16741 ggaagtggtg accaatggtc tgaatatcgc cggtggcatg atcgtggtgg ttggttatgc
    16801 gatggttatc aacatgatgc gtgctggcta cctgatgccg ttcttctacc tcggcttcgt
    16861 aaccgcagca ttcaccaact ttaacctggt tgctctgggt gtgattggta ctgttatggc
    16921 agtgctctac atccaactta gcccgaaata caaccgcgta gccggtgcgc ctgctcaggc
    16981 agctggtaac aacgatctcg ataacgaact ggactaacag gtgagcgaaa tggttgatac
    17041 aactcaaact accaccgaga aaaaactcac tcaaagtgat attcgtggcg tcttcctgcg
    17101 ttctaacctc ttccagggtt catggaactt cgaacgtatg caggcactgg gtttctgctt
    17161 ctctatggta ccggcaattc gtcgcctcta ccctgagaac aacgaagctc gtaaacaagc
    17221 tattcgccgt cacctggagt tctttaacac ccagccgttc gtggctgcgc cgattctcgg
    17281 cgtaaccctg gcgctggaag aacagcgtgc taatggcgca gagatcgatg acggtgctat
    17341 caacggtatc aaagtcggtt tgatggggcc gctggctggt gtaggcgacc cgatcttctg
    17401 gggaaccgta cgaccggtat ttgcagcgct gggtgccggt atcgcaatga gcggcagcct
    17461 gttaggtccg ctgctgttct tcatcttgtt taacctggtg cgtctggcaa cccgttacta
    17521 cggcgtagcg tatggttact ccaaaggtat cgatatcgtt aaagatatgg gtggtggttt
    17581 cctgcaaaaa ctgacggaag gggcgtctat cctcggcctg tttgtcatgg gggcattagt
    17641 taacaagtgg acgcatgtca acatcccgct ggttgtctct cgcattactg accagacggg
    17701 caaagagcac gttaccactg tccagactat tctggaccag ttaatgccag gcctggtacc
    17761 actgctgctg acctttgctt gtatgtggct actgcgcaaa aaagttaacc cgctgtggat
    17821 catcgttggc ttcttcgtca tcggtatcgc tggttacgct tgcggcctgc tgggactgta
    17881 agactgttgt acactaccgg ggccttttgg ccccgttttt ttatctggag gattaatgac
    17941 aatcacggac ctggtactga ttcttttcat cgccgcactc ctggccttcg cgatctacga
    18001 tcagttcatc atgccccgcc gtaacggccc cactctgctg gcaattcctt tgctccggcg
    18061 tggtcgcatc gatagcgtta tcttcgtcgg attgattgtc attcttatct ataacaacgt
    18121 cacgaatcat ggcgcgctaa taacaacatg gttattaagt gcactggctc tgatgggttt
    18181 ttatatattc tggatccgcg ttccgaagat catctttaaa caaaaaggtt ttttcttcgc
    18241 caatgtctgg attgaatata gccgaatcaa agcgatgaac ttgtcggaag atggcgtgtt
    18301 ggtgatgcaa ttagaacagc gtcggctgtt aatccgcgtt cgaaatatcg acgatctgga
    18361 aaaaatttat aagcttctcg tttcaactca ataagttatc aatttagcca aagctatgtt
    18421 tagtgtattt ttaataatca gacatagctt aggctatatt accccttccc ttatttgtta
    18481 tttatcttaa cgtttcattg atatataaat ctaaatgaaa atcgttatca ataaagcaat
    18541 gaaataatat attccaacag ttgttttata ttctcaaaat atgttaaggt tgcgccctca
    18601 tttggggagt agccgatttc cagattccgg aaatgtacgt gtcaacatac tcgttgcaaa
    18661 acgtggcacg tacggactga acactttcag tcaggcgaga ccatatgcac atcaatcgct
    18721 atgcctgcat gaacgcgaaa accatcgcgg tttgttgttc atgtatgcat gtttgctggg
    18781 ggcgatgatg tgttttatgg ataccccggt caggacgttg tcatgaatat cactgctact
    18841 gttcttcttg cgtttggtat gtcgatggat gcatttgctg catcaatcgg taaaggtgcc
    18901 accctccata aaccgaaatt ttctgaagca ttgcgaaccg gccttatttt tggtgccgtc
    18961 gaaaccctga caccgctgat cggctgggga atgggcatgt tagccagccg gtttgtcctt
    19021 gaatggaatc actggattgc gtttgtgctg ctgatattcc tcggcgggcg aatgattatt
    19081 gagggttttc gtggcgctga tgatgaagat gaagagccgc gccgtcggca cggtttctgg
    19141 ctactggtaa ccaccgcgat tgccaccagc ctggatgcca tggctgtggg tgttggtctt
    19201 gctttccttc aggtcaacat tatcgcgacc gcattggcca ttggttgtgc aaccttgatt
    19261 atgtcgacat tagggatgat ggttggtcgc tttatcggct caattattgg gaaaaaagcg
    19321 gaaattctcg gtgggctggt actgatcggc atcggcgtcc agatcctctg gacgcacttc
    19381 cacggttaat aagaacgctg ccagaggtga atattaaagt ccgtctggca gtcgaacact
    19441 tcttttgctg ccagtgtttg ccagacttct gacttcgcac gccaggcaaa cggcgtcatc
    19501 tgcaataatg cgacggcttc gtcaccgcga agacgcatcg gataacacaa ctcagcactc
    19561 tgctgtaatg taaaaccttc cagttgttct gcatgaggtg catgaagatg tacttcattg
    19621 taaatcagcc ccttcagctc catcaaatgt cgcggtcccg gcgtggcagt aatgacccaa
    19681 ccgccaggct tcactactcg cgctaattct tctgctttac acggcgcgta aatacgtatt
    19741 atggcgtcca tactggtatc ggaaaacggc aaacggtggc tggaagcaac acaaaaagtg
    19801 acctgcggat agcgtttcgc cgccgctttt atcgctacct tcgagacatc caggccaaac
    19861 gtggtgattt cgggcagcgc atcggcaaat gcgtgtgtgt aatacccttc accacagcca
    19921 atatccagca ccgctgtggc cttttcatca agccgttccc gcagttgggc gacaattgca
    19981 tcacgcagcg gctgataatg tccggcatct aagaatgcgc ggcgtgcttg catcatttcc
    20041 gcgctgtcgc ccggatcacg agaccgttta tgctgaacgg gcagcagatt gacataccct
    20101 tctttcgcca tatcaaactg atgtcgctgg ggacagatat agctgttttt ttcacgcgaa
    20161 agaggctgat ggcaaagtgg acaggaaaac gacatgacaa ctccggcagg atactaaagg
    20221 ccgcaagtgt aacgcgaatc acgcggcggg caaatatccg aacgactgga gcgaagataa
    20281 agcaaagtaa aaagcctcgc attcgacgag gctttatagc gagattgaag cgtattcaca
    20341 cttcagatca gtggattcga tcagatagct gttacgttaa cagctgccgg acctttctga
    20401 ccgtcctgaa tttcgaactc aacgttctga ccttcagcca gagttttgaa accattaccc
    20461 tggatagcag agaagtgtac gaacacgtct ttgctgccat cagccggagt aatgaagcca
    20521 aaacctttag actcgttgaa ccacttaacc tgacctttaa tctttgccat ttgaaaaatt
    20581 ccttagattg ttttcttcgc ccgcaggcat aacatagata aaactgacac attactgcat
    20641 gaggcaccaa tataaggctc ggcagagaag cggtattcaa cgtcaacgtg tttactcagg
    20701 acttctttac tgaaaatgcc acacataaac agaactgtac ctcgtttaac ccgaaatctg
    20761 ttatcacata caacgttaat tatggcaagc catttttaaa catgtctcga tcagacgcac
    20821 aaatctcgat acgtcttcca ctttttttgc acacttatgc aacggaatac gcgccgatat
    20881 atcattgtgc ttaaccttgc cagttcaggc agatacttaa actgacgtat tttctaacat
    20941 agttcaataa gttcagttgt tccaggccag tgggagaagt tattacatag tgcgtgcaat
    21001 atcacatttt ctgctatgca atgaataaaa agttatatca ctttttctca taaaacagtc
    21061 agttaacggc tatcgatcac cccaaagaga agtcaatcgc caaaggtatt gtaaatttaa
    21121 aataagaaaa attgatgaat gcgcaaaaaa atcaagagag aaacgtttcg ctaataaata
    21181 ataccacact taatcacaaa tccgatgtaa acatcctaat aaattaatgg gctaaatatc
    21241 tttttctgat atcccgttac gcaaataaat aatatgataa ttatttgaag tcagaccaga
    21301 gttttaattt gaagcagttg caccatggca gtgcgtataa tcaggatgaa ttgagtaacc
    21361 cttcaacgac ggggttactc aagattgagg aaggattatc gttcggcgac cagacgaata
    21421 atatcttcgt cttcaaccga tttttgactc tcaacggtct ttttatctgc tttctcaggt
    21481 tcgtttgctt cgcacaaacg acgtaacaga gcattttgac gtttttgctg atccagcaac
    21541 gcctcaagca gttcaatctg ttcgttagtc cgtgaactgg cacgattgat aaaaaaccac
    21601 aagatgagcc cgataagaag aaccaccacc gatacaacca aagacgcaat gttcatcacg
    21661 cctgaattta caacttcgtt catttcacca cctctgagta gaggcgctat tctaccactg
    21721 ctggagagga agaaaatcta gtgctgaaaa aatgatatca ccacgggata aactggttaa
    21781 tggcacaaat tccgctgaaa aattgtacat cctgatcgca catcatgttg aatacctgcg
    21841 cccaaagcag caagcaagcc aacaccacga caaccagaac gacccatcga aactttttca
    21901 ctccaatctc cgtttcacct accttatgtc attaaactag catgcgttag ttaaacagcg
    21961 cctaactatg tcgatattcg tgctttttca gaatgagtcg cttgcttcag cgcatattgc
    22021 cgctacgatt aagcgaacat aaaaagagaa gaggttgtaa tgcgattcat cattcgcaca
    22081 gttatgctga tagcactggt atggatagga ttattactca gcggatatgg cgttttgatt
    22141 ggaagcaaag agaatgccgc gggattaggt ttgcaatgta cctatctgac cgccagaggc
    22201 accagcacgg tacaatacct gcacactaaa agtggttttc tggggataac agattgcccg
    22261 ttgttacgta aaagcaatat tgtggtcgat aatggttaag ggtaataaat aaccagattc
    22321 tcactcatga gtaaaatatt cttatcgcaa gtgcgtttaa tataacgcca ccttcaactt
    22381 tatttttctc atgctaaata aagaacctgt ctgtttgata ttcagacagg ttcttactct
    22441 catcagaatg gatagtcgtg ataacccatt tgggcagaaa ttttgcgcgc cgcggtatgc
    22501 aacattgcga catattcctg taaacgctct tcggagaaac gcaacgtcgg gaaggagatg
    22561 ctcagaccgg caatgaccac gccaaagcga tcaaataccg gaaccgcaat gcaacgcagt
    22621 ccttcttcct gctcttcgtt atcttcgcca tacccctgct cgcgcacctg gtccagaacg
    22681 ggtaataacg attctgtact ggtgatggtc cgttcggtac tgcgtttata ctccacgccc
    22741 tcaagaattt gcttcacttc atcgcgatcg cgccatgcca gcagtacctt accaattgcg
    22801 gtgctgtaca gcggattacg acgcccaatc cgtgaataca tgcgcaaatt gtacatagag
    22861 tcaattttgt gaatgtaaac aatactgtct tcgtccagtg cgccgaggtg gatagtttct
    22921 ttggtcaggc gggaaatctc acgcatctgg atatctgcgc tacgaattaa atcgacgttt
    22981 tgtaacgcgc gagcgcccag ttcaaacaat ttcagggtca gggaatattt ctccgactcc
    23041 ccttcctgcg ccacataacc taaggttttc atggtctgta aaaagcgata aacggtgctt
    23101 tttgacatca tgacgcgctg cgacagctcg gttatcccta tttcgcgctc ttcacccagc
    23161 gcctgcaaaa tgccaaaaac tttcagcacg gaagatacag aatcaggctg tttatccaga
    23221 tctgcgttag ccatttatca cctcattgcg agtgttttat aaaaatcaga actgtttttt
    23281 attataattt cgcaccaggg tggtcgcaat ccatcttttg ccggttagtt acaattctgc
    23341 gacatccacc gtgaatatca gtgctagaat catacccctg ttgattattc accaaagata
    23401 taaaattcct atgccaaaag ttcaggccga cggcctgcca ttgccccagc gatacggtgc
    23461 gatattaacc attgtgattg gtatttcgat ggctgtcctt gacggcgcaa tcgccaacgt
    23521 cgccctgcca acaatcgcca cggaccttca tgccacgcca gccagttcca tctgggtagt
    23581 gaacgcctat caaatcgcca ttgtcatctc cctgctctca ttttcgtttc tgggcgatat
    23641 gtttggctat cgacgtattt ataaatgcgg tctggtcgtt tttctgttgt cttcactgtt
    23701 ctgcgccctt tctgattcgc tgcaaatgct cacccttgcg cgtgtcatac aaggtttcgg
    23761 cggtgcagcg ttgatgagcg ttaataccgc acttatccgc ctgatctatc cacaacgttt
    23821 tctgggtaga gggatgggca taaactcgtt tattgttgcc gtttcttctg ctgccgggcc
    23881 gacaattgct gcagcaatcc tctccatcgc atcctggaaa tggttatttt taatcaacgt
    23941 accgttgggt attatcgccc tgcttctggc aatgcgtttt ctgccaccca atggttctcg
    24001 cgccagtaaa ccccgtttcg acctgcccag cgccgtgatg aacgcgttaa ccttcggcct
    24061 gcttatcact gcgttgagtg gtttcgctca ggggcaatcg ctgacattga ttggtgcgga
    24121 actggtggta atggttgtcg ttggtatttt ctttattcgc cgccagcttt ctcttcccgt
    24181 accgctgcta ccggtggatt tactgcgtat cccgctgttt tcactttcta tttgcacatc
    24241 tgtttgctct ttctgcgcac aaatgctggc aatggtttcc ctgccctttt acctgcaaac
    24301 cgtgctcggg cgtagtgaag tcgaaacagg tttacttctg acaccgtggc cgttagcaac
    24361 gatggtgatg gctccgctgg caggctattt gattgaacgc gtacatgcag gattgctggg
    24421 tgctttagga ttattcatca tggctgcggg gcttttttcc ctggttctgc tgcccgcgtc
    24481 acctgcggat atcaatatta tctggccgat gatcttatgt ggcgctggat ttggcttatt
    24541 tcagtcaccc aataaccaca ccattattac ctccgctccg cgcgaacgta gcggtggggc
    24601 cagtggcatg ttagggacgg ctcgtctatt gggtcagagt agtggcgcgg cgctggtggc
    24661 gctgatgcta aatcagtttg gtgataatgg tactcacgtc tcgctgatgg ctgcggctat
    24721 tctggcagtg attgctgcct gtatcagtgg gttacgtatc actcagccaa gatccagggc
    24781 ataaaaaaag cgcgtcgagc aggacgcgct ttttagtatt tacttcatat tacttcaggt
    24841 attcacccgt acgcagagct tcaatacgtt tatccagcgg cgggtgagtc atgaacaact
    24901 cactgagcga tttcgactta ccgttaatac agaaagccat catgctggtt gcttcttgcg
    24961 gttcatagct ggttttcagg cgctgcagcg cggcaatcat tttctcgcga ccaaccagtt
    25021 ttgccgaacc ggcatcagca tggaattcac gatgacgcga gaaccacatg gtgataatgc
    25081 tcgccagaat accaaacacc agttccagaa ccgttgcaac cgcaaagtag atcagcgggt
    25141 tgccgttgct ctcttcacct tcatcacgat ttccgcccat aaaacccgcg gcaagctgcg
    25201 ccagaatacg ggaaataaag ataacgaagg tgttcaccac gccctgaatc agcgtcatgg
    25261 tgaccatatc accattggcg atgtggctga tttcatgagc aattaccgcc tcggcttcat
    25321 ccgggctcat gttctgcagc aaaccggtgc tgacagcaac cagagaggca tcacggcgcg
    25381 caccggttgc aaaagcgttg atgtccggcg catggtagat agccacttgc ggcatagcga
    25441 tccccgcctg acgagcctgg gttgctacaa tattgaccag ccaacgttcc ctttcgttac
    25501 gcggttgctc gatcacttcc ccgccaacag atcgtaatgc catccatttg gacatcagaa
    25561 gcgaaacgaa ggaaccacca aaaccgaaca gcaaggccat gatcatcagc ccctgaacgc
    25621 tgctcgactg tatccctgtc aggctcagta ccagcccgaa aacgaccatt acggccaggt
    25681 tcgttagcag gaagagcgcg attcgcatca taattttctt tttacctcag tttaacaaaa
    25741 cgcaatatgc gatacccaca tcgtatgggt tacgcgacta ttttcaagtc tggatagtgc
    25801 gtaagtcacc agaaagacac aactttacat tttgtagcat ctgatttacg gcatcttgcc
    25861 gctgttaaaa aatcaggcac aatttcttgt gcctgattga tattacttga cgggagcggg
    25921 ttgttccgcg ggtctggctt tttcaagctt cgccagatcg agtgcgatat tcaccgtctc
    25981 atccagataa ggatccggct cctggtaatc tttcggtaga tcatccagtt tcttcaactc
    26041 cggtttacct tcgcgtttaa agcgttcgtt caaacgcgcc agacgcgtcg catcatcttc
    26101 attattctct ttctcacgca cagcgtaatt cagagaaacg atattgcgct tgtccttcat
    26161 agcgttgaag cgcgcgatat ccttcatgat gttctggaac tcaggatctt tcgcgatacg
    26221 cgcattatgt tccttcagca gctccggtcc aaaggccgtc aaatctcccg atttcacata
    26281 ggtcgcggca tcgatgctat cccacggcag cgcgttatct tcgaatttct caccggtttc
    26341 cgtttcttca ttacccgtcg gcatgatgat gtctggcgtt acgcctttac gttgcgtact
    26401 gccgccgtta acgcgataga atttctggat cgtgtactgc acagaaccca gcgctggcca
    26461 ttcaggacgt aacatctgat cgtaaatacg gttcaatgaa cggtattgct gaacggtgcc
    26521 tttaccaaac gtcggttcac ccacaaccag cgcacgaccg taatcctgca ttgccgcggc
    26581 aaagatttct gaagccgaag cactgaagcg gtcaaccagc accaccagcg ggcctttata
    26641 gaaaacctgc ccatcggtat cgctgtcttc acgaacttta ccgttgttat cacggacctg
    26701 aacaatcgga ccggatggaa tgaacagacc ggagagcgat accgcttcag tcaacgcccc
    26761 accgccattg ctacgcaggt cgatgatgac actgctgaca ttctgttttt ccagtttctg
    26821 caactgcact ttgacatcgt ctgtcaaacc cacatagaaa cctggaatat ccagaacgcc
    26881 gactttctct ttaccgacgg tcttcaccga cattttaacc gcacggtctt cgaggcgaat
    26941 acgttcacgg gtcaacgtta cagtacgggt cttggtccct ttaccagcag gtaaaatttc
    27001 cagacgaact ttactgccct tcggcccttt aattaaggca accacatcat caagacgcca
    27061 gccaatcacg tcaaccatcg gcttgcctgt ttgaccaaca ccgacaattt tgtcaccaac
    27121 gctgatagct ttactcttcg ctgccggacc acctgccacc atcgaattga taacggtgta
    27181 gtcatcatcc atttgcagca ctgcgccaat accttccagc gacaaactca tttcagtgtt
    27241 gaactgttcg gtattacgcg gggaaagata gttggtatgc gggtcgattt cacgcgcaaa
    27301 cgccgtcatt gccagcgaga aaacatcttc gctgttggtt tgcgccagac gacgaatggc
    27361 aaatttgtag cggcgagtca gggtttcacg aatttcttta tccgtttttc ctgccagctt
    27421 caggcttaac tcgtcgaatt tgactttact gtcccacagc gcgttcaact cagcctcgtt
    27481 tttcggccat ggcgctttgc tgcggtcaag gttataagtg tcgttgccgg taaaatccat
    27541 cggcttttcc agtaccgaca aagcgtactg gtaacgctca aaacggcgct tttgcgccag
    27601 attgtagaga tcgtagaaaa cgtcgagttt tcctgaacgc agttcatcac ctaactcggt
    27661 tttctttttc gcgaactgtt caacatcgct tgccagcagc acgttgtggc tgtaatcgag
    27721 cagattcagg tagcggtcaa agattttggc cgaaaatgcc tgatcgaggt cgaactggcg
    27781 ataatgagaa cgggtgaagc gcgacgttac gcgctcactc accgtcgcat gctgcgtctc
    27841 ttcctttaat accggaattt gatcagcacg cgtgatatct tctacagcga aggtctggcc
    27901 tgctattgca agcaggccag ctaacgcggt aagcctaaaa aacatgttca tgcctggccc
    27961 ggcctccgtt tcagaacacc aggtgttctg cgcgcacaat caaagacata cccgaattca
    28021 gctggacgcg gacgccgtct ttggtgattt ctaatacggt ggcatccatc gcgttttgac
    28081 ccgctttcac cttcagggct tgtccgacag tcagagctga aatgtcagaa accggggtgt
    28141 gctgttcttc gcgaggtgct tttactgttt ttggcgcttt ctctaccggc ttttgcgcac
    28201 gaggtttacg ttcagcgcct tctttgcggc gtggcgtggt cggacgtggc ttgcgttcgc
    28261 ggcgcggtgc gtcttctttc tcaccagcag ctgcggcagc ttcgcgtttt ttcgcttgct
    28321 gctcagcacg ctgtgcctga acacgcgctt tcgcttcttc aagctgcttg cgagcatgct
    28381 ctacatgttg ctcgtccagc tcaccgcatg ggttgccgtc aagatcgaca cgcgttgcgc
    28441 cgggtttaac accgtaaaga taacgccagc tcgaagtgta gagacgtaaa gcggatcgca
    28501 attgcgtttt gctcaggttc atttccccgg caacacgatc gaccaaatcc tgaaaaatac
    28561 cgattttcag cggacgcgct tcaccttccg cactgaaaca gtggggaaaa cgttcggcca
    28621 gaaacgcgat tacttcttta ctgctattca acttaggttg attttccatg aaatttcctg
    28681 attacaacgg acgtagccaa caagcgcagg catgaacagg cgtcattata atgacgctac
    28741 cagtaaatgc tacgttatcc gttgattatc ctgcgacgct cgcaaagaat tttttgtaat
    28801 ccgtcgttgc aagcactttt tcaagctgtg ccacaagctg acgtaagcct tgctcgtcct
    28861 cgtctgtaaa gcgaccgaag acggtactat cgatgtcgag aacaccaata atctgatttt
    28921 tcaccaccag cggcagaaca atttcagaat tactcgccgc atcacaggca atatgcccgt
    28981 caaacgcgtg aacatcctcg atacgctgca cttgattgcg ggcaaccgca gtgccgcaca
    29041 ccccgcgccc gacgggtatc cggacacagg caattttgcc ctgaaatggt ccgagtacca
    29101 gtgtatcgtc ctcaagcaaa taaaaacctg cccagtttac gtcagtgaga cgctcgtata
    29161 acaacgcact ggtgttcgca agcgttgcca gaaaactggt ttctcctgcc atcagcgcgt
    29221 taaagtcgcg atttaaatcc gcgtaaaatt ctgttttgtt cattatataa tcacttggtt
    29281 gtcttacctg gaactgccag cctattaaaa taagcattaa atgcgttaat gctcaagatc
    29341 attcccatca tgggttaaga ttaatgttaa ttcttattac atttggcacg tcatggctct
    29401 taacacacca caaattacgc cgacaaaaaa gataacagtg agggctatcg gtgaggaact
    29461 gccgcgtggt gattaccaac gttgcccgca atgtgacatg ctgtttagcc tgcccgagat
    29521 aaattctcat caaagtgcct attgtccgcg ctgtcaggca aaaattcgtg acgggcgcga
    29581 ctggtcgcta acgcgcctgg cggcaatggc cttcaccatg ctgttgctga tgccgtttgc
    29641 ctggggcgaa ccgctgttgc atatctggct gttaggcatt cgtattgacg ccaacgtcat
    29701 gcaaggcatc tggcaaatga ccaaacaggg cgatgcgata acgggatcaa tggtcttttt
    29761 ttgcgttatt ggtgcccccc tcattctggt gacctccata gcttatttat ggtttggtaa
    29821 ccgactggga atgaatctac gtccggtact gctgatgctt gagcgactga aagagtgggt
    29881 aatgctcgat atctacctgg tcggcattgg cgttgcttct ataaaggtgc aggattatgc
    29941 ccatatccag acgggtgtcg gcttgttctc ttttgtggcg ttggtgattt taacgacggt
    30001 gacgttgtca catctcaatg tcgaagaact gtgggagcga ttttatccgc agcgccccgc
    30061 tacgcgtagg gacgagaaac tccgtgtctg tcttgggtgc cattttaccg gctatcctga
    30121 tcagcgtggt cgctgcccgc gttgccatat cccgctacgc ctgcgtcgcc gtcatagtct
    30181 gcaaaaatgc tgggcggcgc tgttagcgtc aatcgttttg ttgttacctg ccaacctgtt
    30241 gcctatttct atcatttatc tgaacggagg acggcaggaa gatacaattc tttccggaat
    30301 tatgtcgctg gcaagtagca acattgccgt tgcgggaatc gtgtttatcg ccagtattct
    30361 ggtaccgttt actaaagtga tcgtcatgtt cactttactg ttgagtattc attttaaatg
    30421 ccagcaaggt ttacgcacac gcattctgtt actgcggatg gtgacctgga ttggtcgctg
    30481 gtcgatgctc gacctgtttg tcatatcttt aaccatgtcg ctgattaatc gcgatcagat
    30541 cctcgctttt actatgggac cggctgcgtt ttatttcggc gcagcggtaa ttttgactat
    30601 tcttgctgtg gaatggctgg acagccgctt actttgggat gcacatgagt caggaaacgc
    30661 ccgcttcgac gactgaagcg cagattaaaa ataaacgccg tatctcacct ttctggctgc
    30721 tgccattcat cgcgctaatg attgccggtt ggctgatttg ggatagttat caggaccggg
    30781 gtaataccgt caccatcgac tttatgtcgg cggatggtat tgtcccgggc cgcacccctg
    30841 ttcgttatca gggcgttgaa gtcggaactg tgcaggatat cagcctgagc gacgatcttc
    30901 gtaagattga agtcaaggtc agcatcaagt ctgatatgaa agatgcgctg cgcgaagaga
    30961 cacagttctg gctggtgacg ccaaaggcct cgctggcagg tgtctccggg ctggatgccc
    31021 tcgtcggtgg taactatatc ggcatgatgc cgggtaaagg taaagagcag gatcactttg
    31081 tcgcactcga tacccaaccg aaatatcggc tggacaatgg cgatctgatg atccacctgc
    31141 aagcccccga tctcggttcg ctgaacagcg gttcattggt ctatttccgc aagatcccgg
    31201 tcggaaaagt ctacgactat gccatcaatc ccaacaagca aggcgtggtg attgatgtcc
    31261 tgatcgagcg gcgttttacc gacctggtga aaaaaggtag ccgtttctgg aacgtttccg
    31321 gcgttgatgc caacgtcagt atcagtggcg cgaaggtgaa actggaaagt ctggcggcac
    31381 tggttaacgg tgcgattgcc ttcgattcac ctgaagagtc gaaacctgcc gaggcggaag
    31441 atacctttgg tctgtatgaa gatctcgccc acagccagcg tggcgtaata ataaaactgg
    31501 aactgccgag tggggccgga ttaaccgccg actcgacgcc gttaatgtat caggggctgg
    31561 aagtcggaca gctgactaaa ctggatttaa atcctggtgg taaagtcact ggggaaatga
    31621 ccgttgatcc cagcgtcgtc accctgcttc gtgaaaatac ccgcatcgaa ttacgcaacc
    31681 cgaaattatc ccttagcgat gccaatctca gcgccctgct gaccggaaaa acctttgagt
    31741 tggtgcccgg cgatggcgag ccacgcaaag agttcgttgt tgtgcccggc gaaaaagccc
    31801 tactgcaaga gccggatgtt ctgacactga ccctgaccgc gccggaaagt tacggtattg
    31861 atgctggtca accgctcatt cttcacggcg tgcaggtagg ccaggttatt gatcgtaaac
    31921 tcaccagcaa aggcgtcacc tttaccgtcg ccatcgagcc tcagcatcgc gaactggtaa
    31981 aaggcgatag caaatttgtc gtcaacagcc gtgtcgacgt gaaggtgggg ctggatggcg
    32041 ttgagtttct cggtgccagc gcctcagaat ggattaacgg cgggatacgt attctgccag
    32101 gcgataaagg cgagatgaaa gccagctatc cactgtatgc caatctggaa aaagcgctgg
    32161 agaacagcct tagcgattta cccaccacaa ccgtgagttt gagtgcagag acgctgccgg
    32221 atgtgcaggc aggatcggta gtgctgtacc gtaaatttga agttggtgaa gttattaccg
    32281 tgcgtccgcg agctaacgcg tttgatatcg atctgcatat taagccggag tatcgcaacc
    32341 ttttgaccag caacagcgtg ttctgggcag aaggtggggc gaaagttcag ctgaatggta
    32401 gtggcctgac cgtacaggca tccccgctct ccagagcatt aaagggggct attagcttcg
    32461 ataacctcag cggtgccagc gccagtcagc gtaaaggcga taagcgtatt ctgtatgctt
    32521 ccgaaacagc agcccgtgcg gttggcgggc agattacgct tcacgctttc gatgccggaa
    32581 aactggcggt cgggatgcca attcgctatc tcggtattga tatcgggcaa atccagacgc
    32641 tggatctgat taccgcgcgc aatgaagtac aggcaaaggc ggtgctctat ccggaatatg
    32701 tccagacctt tgctcgcggt ggtacgcgct tctcagttgt cacaccgcaa atttcggcag
    32761 ctggcgttga gcatcttgat actatcctcc agccgtatat caacgtcgaa ccaggacggg
    32821 gcaaccctcg ccgcgacttt gaattacaag aggccaccat aactgattcg cgttacctgg
    32881 atggcttaag cattattgtt gaagcgccgg aagccggttc gttaggcatc ggtacgcctg
    32941 tgctgttccg tggtctggaa gtcggtacgg ttaccggaat gacgctgggg acattgtcag
    33001 atcgcgtgat gattgcgatg cgcatcagta aacgctatca acacctggtg cgtaacaatt
    33061 ccgtcttctg gttggcatcg ggttacagtc tggactttgg tctgacgggc ggcgtcgtga
    33121 aaaccggcac ctttaaccag tttatccgtg gcggcatcgc cttcgctacg cctccgggta
    33181 cgccgctggc accgaaagcc caggaaggca agcacttcct gttgcaggaa agtgaaccga
    33241 aagagtggcg tgaatgggga actgcgcttc ccaaataatg cccactgctc cggcgtgcct
    33301 gcgccggagc gtttatgcta aactgcgcgc ctgttttttt gccagtggta catgctcgtg
    33361 gcccaacaca ccgtttattt cccggacgcc tttctgacac aaatgcgcga agctatgcct
    33421 tcgacgctct cttttgatga ttttcttgcc gcctgtcagc gcccgttgcg ccgcagcatt
    33481 cgcgttaata cgctgaaaat ctctgttgct gatttcctgc aattaaccgc tttttatggc
    33541 tggacgctta cgccaattcc gtggtgtgaa gaaggtttct ggattgaacg cgacgatgaa
    33601 gatgcattgc cgttgggtag taccgccgag catttaagtg gcctgtttta tattcaggaa
    33661 gccagttcaa tgttgcccgt cgccgccttg tttgctgacg gtaatgcacc acagcgggtg
    33721 atggatgtcg ctgctgcgcc aggctccaaa acgacgcaaa ttgccgcgcg gatgaataac
    33781 gaaggggcta tccttgccaa tgagttttcc gccagtcggg taaaagtgtt acatgccaat
    33841 atcagccgct gtggcatccg taatgttgcg ctcacacatt ttgatggccg cgtgtttggt
    33901 gcggcattgc cagaaatgtt cgatgccatt ttgctggacg ctccctgctc cggcgaaggc
    33961 gtggtgcgta aagatcccga tgcgctaaaa aactggtcac ccgaaagcaa tcaggaaatc
    34021 gcagctaccc aacgggaact gatcgacagc gcctttcatg cattacgccc tggcggtacg
    34081 ctggtttact cgacctgtac cttaaaccag gaagaaaatg aagccgtctg ccggtggctg
    34141 aaagagactt accccgacgc agtagagttt ttaccgcttg gcgatctctt ccctggtgca
    34201 aataaggcgc tgaccgaaga cggctttttg catgttttcc cacaaattta cgactgcgaa
    34261 ggcttcttcg ttgctcgtct gcgtaaaact caggcgatcc ccgtcttacc cgccccaaaa
    34321 tacaaagtgg gcaatttccc gtttagcccg gtgaaagatc gcgaagccgg tcaaattcgt
    34381 caggcggctg caagtgttgg cttaaactgg gatggaaacc tgcgactctg gcaacgcgac
    34441 aaagaactgt ggttgttccc ggtaggcatt gaagccctga tcggtaaagt ccgattttct
    34501 cgcctgggga ttaaacttgc cgaaacgcat aacaaaggtt atcgctggca gcatgaagcg
    34561 gttattgccc ttgcctcccc cgacaatgtg aacgcttttg aactgacacc gcaggaagcg
    34621 gaagagtggt atcgcgggcg cgatgtttac ccgcaagccg cgccagtagc ggatgatgta
    34681 ttggttactt tccagcatca gccgattggt ttagccaaac gaattggttc gcgactgaaa
    34741 aacagctacc cgcgtgaact ggtgcgagac gggaaacttt ttaccggtaa cgcctgacag
    34801 cgcacaaaaa aagcgcactt tttgactggc acattcggct gcctcaacta ggctgaaaaa
    34861 tggtgcgatc ggactggtcg taccacaatc ggcagctaaa tggagagcac gaagatgaaa
    34921 accagtgtgc gcataggcgc ttttgaaatc gacgacggcg aattacacgg cgaatcgccg
    34981 ggtgatcgaa cgttaaccat tccttgtaaa tcagaccccg atttatgtat gcaactggat
    35041 gcctgggatg ctgaaaccag tattcctgcc ctgctaaatg gcgaacactc tgtcctttac
    35101 cgtacccgtt acgatcaaca atctgatgcc tggattatgc gtcttgccta atccaaaaag
    35161 aacccgtcgg catggcgggt tatttgtcct ggttattccc ccgttgtaaa atctctccta
    35221 aacttaacgg tacggcacca cacttcgggg atgaaatgtt cgcgctggta ctttttgttt
    35281 gctacctgga tggcggttgt gaagatattg ttgtggatgt ctacaacacg gaacagcagt
    35341 gtctttattc tatgagcgat caacggatcc gccatggcgg ttgttttccg attgaggatt
    35401 ttatagatgg tttctggcga ccagcacagg agtatggtga tttttaatta ttgcagttgc
    35461 acaagagtca gttcgccccc aaagacagcg ccggtatcaa tataatgcag gttgccaata
    35521 tccacgcgat ggcgcaacgg tgtatgacca aaccagaaat gatcagcacc tgtaattccc
    35581 tgcccttttt ggcgttcacc taatcgcgag cggctccaca agacctgatg caaatcaacg
    35641 tccttttgcc attcataaac atcatctgga taatcggcat gagcaataac atgtttgccg
    35701 gtgcgactgt gtacttcaag aataaagggc aaatgctgac atttttccag cgtcgttttc
    35761 gcttgtttct gttgattatc tgccagcgca ataaaccagt cgccgccatt catcaaccac
    35821 aaagacatct gctgagatgt cagcgcatcc atcgccatct gttcatgatt gcctcttacc
    35881 gcacgaaccc aatgttgttc cagtaactgc aggcaacgta aactttgcgg cccacgatcg
    35941 ataacgtctc ccactgagat aagtaaatct cgccacggat caaaaccaca atgccataat
    36001 ttgcggcgca actgctcaag acaaccgtgt atatcgcctg aaagccagat atgtcgccat
    36061 tgatgacccg caattctctg ataaacgggc gcaggctgtt tcatcaatat tttcctcccg
    36121 cgctaaagat cacataatct taacaagaat gttaaaaaac gctggactca gacagtagag
    36181 tgtgtgttat ggttgactat aaagtcagcg aagggaatgc ttctggcttt taacagataa
    36241 aaagagaccg aacacgattc ctgtattcgg tccagggaaa tggctcttgg gagagagccg
    36301 tgcgctaaaa gttggcatta atgcaggctt agttgccttg ccctttaaga atagatgacg
    36361 acgccaggtt ttccagtttg cgtgcaaaat ggtcaataaa aagcgcggtg gccatcagct
    36421 taaatgttaa aaaccgcccg ttctggtgaa agaactgagg cggttttttt attggaaatc
    36481 aaaaggctat tttaggtaat taacagagtt tttcagctcg ttctataaac ggtgccagac
    36541 tcattttttc gccgggattg ttaggatcat caatctgaat caccgaaatg ggttgggctt
    36601 tagtcttccc actggcaact tccttttgtg cgatatcgtt taaaggatac tgcacgaggg
    36661 tacttgggtt gatgacatac aaagcattac ccggtcggca agtcagcgtc acctcttcgc
    36721 gattaaacgc ccatttgtct ttacccactt caaaacggct aacggtaatc acctgcggtg
    36781 cagccagcgc cgctgcagaa ctggtgagta acagaaacgc cagaatactt tttttcatca
    36841 ttttcgtaat ccatcaaaaa ggttcgagag tcgcaagcag gctgatgatc accagcacta
    36901 ccgcacctat cgcccattcg agtttagtca tccacacaaa ccagggtgcc gcacgatctt
    36961 catcctgcct catgcgcggt acaagaacat aacgattagc cagcgcgatg accaccataa
    37021 tcatcaccag gatggctttg agcaataaga gttggcccca gtaagtcgta agcgtggggg
    37081 gaaatccggt aatcaacaat gcattaagca cgccgcttgc cagtacgccg atcaccgcaa
    37141 aatgcccgca ccaggaaaaa cgcatcagcg cctgaatagc ctggtgtcgc caacggcctt
    37201 tgatgagttg catacaccag agcaccggca gtaatccccc aaaccacgtc gccgcacaaa
    37261 tcaggtgaat cgcgtgatta gtctgatgga ttttcgccgt taccccttca ttcagcgtcg
    37321 catggccgac tcccgccagc agaataaatt gcgcggtagt gagcataaac agcaaacgtg
    37381 gcatattccg cggttgcata agggcgacga tcaacgttac gagggcgaga acaatttgcc
    37441 ataaccagac gccaccaaac tgcgtttgca aaacagcctg ccaaatgttt ggcgaaaata
    37501 catctgtcca tcccgttccc atcaacccgc cctgaacggc gagcattgca gtggcactga
    37561 tcaaactcca gacggcagcg tgctgttgta aacgcagaaa acgcttcgtc agcaagcgac
    37621 gaatcgttaa gggagccagc caggcaccgt acattgcgaa gccaaagacc aacatcagcg
    37681 aggtgaaatg gataaatcgc agcgcgatcc aggtaaacgc cagcatgatt tatttcacgc
    37741 taaaggtgta gtgtcctttc gttttatgcc cgtccaccga aacaacatgc cagtcaacgg
    37801 tgtaggttcc gggtttcagc gaatcggcca gtggaacaat cagttgcttt tggtcctgct
    37861 cattccgctt cgccggtaat gttttaatat tttcgttttt tggccccgtg atttttgcac
    37921 cactgaatcc tgtttcaacg ccttccgaga agtttaaagt aattgcctgc ggggcagctg
    37981 tcacttgcgc gtttgccgca ggatactgat gcgttaaatg cgcatgtgcc cagacggaag
    38041 gtgtcaccaa cgaagtggtc aggattgcca gcgcgtagcg aagggtgcgt gcagttgaag
    38101 tcatattatc tattcctttt tgtaataact tttttacaga gcataacctt gtctaatgtc
    38161 tgagtcgagg atcatcaatt ccggcttgcc atccaggctc actcttagta acttttgccc
    38221 gcgaatgatg aggagattaa gaatgctgaa gaatctggct aaactggatc aaacagaaat
    38281 ggataaagtg aatgtcgatt tggcggcggc cggggtggca tttaaagaac gctacaatat
    38341 gccggtgatc gctgaagcgg ttgaacgtga acagcctgaa catttgcgca gctggtttcg
    38401 cgagcggctt atcgcccacc gtttggcttc ggtcaatctg tcacgtttac cttacgagcc
    38461 caaacttaaa taaaacttat acagagttac actttcttac ataacgcctg ctaaattatg
    38521 agtattttct aaaccgcacc cataatttgc agtcattttg aaaaggaagt cattatgtcg
    38581 ttctggaaag ttgcagcagc gcaatatgag cctcgtaaaa cctcactgac cgaacaggta
    38641 gctcatcatc tggagtttgt gagggccgcc gccaggcaac aatgtcagct gttagttttt
    38701 ccatcgcttt ctttactggg gtgtgattat tcgcgacgtg cccttcctgc cccacccgat
    38761 ctctcgctgt tagacccgct ttgttatgct gcaacgacct ggcgaatgac catcattgcc
    38821 ggccttcccg ttgaatataa cgatcgcttt attcgtggaa ttgcggtgtt cgcaccgtgg
    38881 cgaaaaacac cagggatcta ccatcagagt catggtgcat gtctgggccg tcgttccaga
    38941 accatcacgg tggtcgatga acaaccggaa ggtatggata tggacccgac ctgttcactg
    39001 tttacgacag ggcaatgtct gggggaaccc gacctgctgg catctacccg ccgcttacaa
    39061 tttttttcac atcagtactc aattgccgtg ctgatggcaa atgcccgtgg taacagtgca
    39121 ctatgggatg aacatggtcg tctcatcgtt cgcgccgatc gtggttcatt attgttagtc
    39181 ggtcagcgtt catcacaggg ttggcaaggc gatatcattc cattacgcta ggctttttcg
    39241 gcctggagca tgccatgttg cgcattatcg atacagaaac ctgcggtttg cagggaggga
    39301 tcgttgagat tgcctctgtt gatgtcattg acggaaaaat cgtcaacccc atgagccacc
    39361 tggtgcgccc cgatcgtcct attagtccac aggcgatggc gattcatcgc atcaccgaag
    39421 ccatggtcgc cgataaaccg tggattgaag atgtgatccc gcactattac ggtagtgaat
    39481 ggtatgtcgc gcacaacgcc agctttgacc gccgcgtact gcctgagatg cccggtgagt
    39541 ggatttgcac tatgaaactg gcccgtcgtt tgtggccagg gatcaagtac agcaatatgg
    39601 cgttatataa aacacggaag ctcaatgtac agacgccgcc gggcctgcat catcaccgcg
    39661 cgttgtatga ctgttatatc accgctgcgt tgcttatcga tattatgaac acctccggct
    39721 ggacggcaga acagatggcc gatatcaccg gacgtccgtc gttgatgacg accttcacct
    39781 ttggcaaata ccgtggcaaa gcggtttccg acgttgccga acgcgatccg ggctatctgc
    39841 gctggttgtt taataacctg gacagcatga gcccggagct gcgtttaaca ctgaaacatt
    39901 atctggaaaa tacttaatcc tcaggcgtag cgggtaatgt tccctgcgcc agcgcgacca
    39961 gaaaagcata ttccatcgct acgccttcgt acgatttaaa gcggccagat ttaccgccat
    40021 ggcctgagtc catgtcggta cagagcaata aaagatggtt atcggttttc agctcgcgca
    40081 atttagcgac ccattttgcc ggttcccaat attgcacctg agaatcgtgt aaaccggtcg
    40141 ttaccagtaa atgcggataa gcctgtgcgg tgacgttgtc atacgggctg tagcttttca
    40201 tgtactcgta atattgcgga tcctgcggat tcccccactc ttcaaactca ccagtggtaa
    40261 gaggaattga ttcatcaagc atcgttgtta caacatcaac aaacggtacc tgggcgataa
    40321 cgccatggaa taattccggg cgttgattaa ttgcaacacc catcaacatg ccccccgcac
    40381 tcccgcccat cgcataacaa agcgaaggag agccatagcc cagctttaac aatgcatcgc
    40441 aggcatcaag ataatcatta aacgtatttt tcttcttcag aaacttaccg tcttcgtacc
    40501 attgttgccc cagttcaccg ccgccgcgaa catggacaat ggcgtagaca aagccacgat
    40561 ctaacaaact caagcggcta aaactgaaat cggcatcaat acttgcgccg taagaaccat
    40621 agccatacac cagcaatggg ttgtgtcctt tgcgaaaatg tttgcgatga tagaccaacg
    40681 aaaccggaac ttcgacgcca tcacgggcga ctatccacag gtgttcactg cggtaattcg
    40741 ccgcatcaaa accaggaact tccgtttgtt ttaatacacg acgctcaccg gtatccatat
    40801 ccagttcaaa cagagtgtct ggtgtggtca tggaagaata accataacgc aatcgcgcgg
    40861 tttcaggttc tggattgtag gcaatccagg tcacataggc cggatcatca aaggcaatgc
    40921 caatgacttc ccgggtcttg cggttgattt ggcgcaaact ggttaacccg cgctgacgct
    40981 cttcaaccac cagccagtcg gtaaacagcg taaacccttc cagcatgatg ttatcacgtg
    41041 gcggaattaa ctcttcccac tgttgctcat cacgcatacg ggtacggtat aagccaaagt
    41101 ttttaccgcg gcggttggaa cgcagataaa accgatgctg gtagtgatca aggctgtatt
    41161 cgtgatcttt gcggcgaggc agaaaaacaa acggctcggc atcggccatt tccgcgtcca
    41221 gcaggcgaac ttcactggtg gtggcgctgg ccagatgaat gaccacataa tgcttcgagg
    41281 tcgttttatg caggctgacg taataggtat cgtctttttc ttcgtagatc agtgtatctt
    41341 gcgatgctgg cgtaccgatg gcgtgacgcc agacctgata aggcagcagc gtaactggat
    41401 gcttgcgtac atagtagaaa gtccaggagt catttgccca gacaaagctg ggttcaacgt
    41461 tatccagcag ttccgggtac cagttaccag tttccagatt acgaaaacga atgccgtact
    41521 ggcgtcggga aagaaaatct tctgccagcg ccataatggt gttatcgggc gtaatcgcca
    41581 ttccgcccat cgaataaaac tcactatgag ctgcgcgctt gttggcatcg agcaatgttt
    41641 cccactcatc ccactcttcg ctgaatgccg attgacgctg gtagatagca tattcacagc
    41701 ctggttcata aatatgccga tagcggtagc cattttttat gtagggagca gaaacttctc
    41761 tttgcggaat gcggtcgatg atttccttta agatgcgatc ctgcaaggct tgttgcgagg
    41821 ccatcacccg atgaccgtaa ctattttctt gttgcaggta gtccaggact tccggctgag
    41881 aacgcgtatc gtcccgcagc cagtagtaat tatcgatgcg cgtatcacca tgaagcgtca
    41941 tggcgtgggg aatgcgggcg gcttttggta gcatgttatt gttctttctg gttgaaacat
    42001 cttataaggg tggcaaaact caccggggat gcaagcgaaa cagggtagtc attgcttaga
    42061 tgatgacagg taatggcgcg gatatcgaat gttatgcaga cagagaaaat cagcctgctc
    42121 aaatggctgt gcggttctgg atagcccgaa atagtcaact tcaggctatc cagagagcgg
    42181 aattattccg ccaaagtgcg tttttgttgt tcgagatcgc gttcaatgcc ttcacgaacg
    42241 tcctggggga ttttcagcgc gtcacccagt gcattcaggt aactgcgttc cataaaatgg
    42301 tcaatatcaa tagccgcgca gctcaggaaa tagatttcca gcgcctcttc ttcattgcgg
    42361 atcccggtag ccaggcgttg tggatccagc ggttgttcga ttgcctgctc aatgagtaca
    42421 cgcccctgtt cttccacacc ggcttcacgc aattgctgat cgattgccgc acgttctttg
    42481 gcatcaatat gaccatcact tttagcggca aagaccagcg caaggatcaa acgttccgta
    42541 cgctcatcca gcggcgtact ttgcgcgcca aactgcggtt cgtcctgatg cgccgcgcga
    42601 attttatctt tgtatttatt ccacagcacc gtacccgcta ccgctccgcc gccaaccagt
    42661 aacgcgttgg tgccatattt cgtaagaagt ttacgtgctg atttatttgc gaccagcagc
    42721 ccagccagtc cgcctaatgc gcctggcact aaaagtttgc ccaatccctg atccgcagac
    42781 gaggaggcag aagaactgct ttgcccaagc aaggattgca gttgatttaa ccagttagcc
    42841 atatttgctc ctcaataacc atttttagtg cctgtagcat agcggatgag gatgtcagaa
    42901 aatgtatatc gagacaaaag atgcgaaaat aagcggggcg gtagaccgtt attcaaagaa
    42961 attcgcgcca ggtaaggtgt ttactgtata tcaggtgata agggtatttt aggtgaaagt
    43021 agtttgacgt tttgagtggc actgtttgac gctgattgcc cgatgcgacg ctgttgcgtc
    43081 ttctcaggcc tacgattgtg gtgcgttgtg gggcggacaa ggcattcgcg ccgcatccgc
    43141 cgacggtcaa tcgaattatc agaggccatt aacgccgctg atattccgcc attcccgctt
    43201 tgcagtccac gctgacctgg taatgaatat cggcactttt accacgtacg gttagcggta
    43261 ctgaccattt atcatcttta ccctgaatgt cctgcaaact gacccatgcc acaggatcgg
    43321 cctgaccgac aattttttga tcatctgccc agcgcgccac gcgattttgt tgataatcac
    43381 gttttacgct cgcggcaatt ccagcagcat ccagaccttc acactttggg aaagtgaccg
    43441 acttgctggt ttcattattg gcagcgaaaa ctgatgcgca agcagaaacc aacaacagcc
    43501 ctaaaaacgc ccctcttttt ttcatgtttt tctccatagc acaatgattc aggagaaagc
    43561 atggtacaaa ttgtcaggag cgcaagttgc ttcaggccgc gtgtgaggca tcttcctctt
    43621 cggattcagc accgacgacc tgtggggacg gtaatttgcc ggttttcaga atggtgctca
    43681 gcacatcttt ttgttctgcc agccatagcg aaagggcttc acgttgctcg tcttccattt
    43741 gcactggaga atttgtcagc caggtgtcaa gcagatccgc cgtatcaagc attttgtcat
    43801 aagcatcggc ttcctttttg ctggtaaacg acattttctc ttcgccctca cgaatgacta
    43861 cgtatttaac ttcaaccgcc atttgcagcc tctcataata actgtgattt tatacagtat
    43921 atttcttttc ggttgggaaa tcaacatcag caataaagac acgcgcaaac gttttcgttt
    43981 atactgcgcg cggaattaat caggggatat tcgttatgac gttattaggc actgcgctgc
    44041 gtccggcagc aactcgcgtg atgttattag gctccggtga actgggtaaa gaagtggcaa
    44101 tcgagtgtca gcgtctcggc gtagaagtga ttgccgttga tcgctatgcc gacgcaccag
    44161 ccatgcatgt cgcgcatcgc tcccatgtca ttaatatgct tgatggtgat gcattacgcc
    44221 gtgtggttga actggaaaaa ccacattata tcgtgccgga gatcgaagct attgccaccg
    44281 atatgctgat ccaacttgaa gaggaaggac tgaatgttgt cccctgcgcg cgcgcaacga
    44341 aattaacgat gaatcgcgag ggtatccgtc gcctggcggc agaagagctg cagctgccca
    44401 cttccactta tcgttttgcc gataacgaaa accttttccg cgaggcggtt gctgaaatcg
    44461 gctatccctg cattgttaaa ccggtgatga gttcctccgg caaggggcag acgtttattc
    44521 gttctgcaga gcaacttgct catgcatggg agtacgctca gcaaggcggt cgcgccggtg
    44581 cgggccgcgt aattgttgaa ggcgtcgtta agtttgattt cgaaattacc ctgctaaccg
    44641 tcagcgcggt ggatggcgtc catttctgtg caccggtagg tcatcgccag gaagatggcg
    44701 actatcgtga atcctggcaa ccacagcaaa tgagccagct ggctttggaa cgtgcgcagg
    44761 agattgcccg taaagtggtg ctggcactgg gcggttatgg tctgtttggt gtcgagctgt
    44821 ttgtctgtgg tgatgaggtg attttcagtg aggtctcccc tcgcccacat gataccggga
    44881 tggtgacgtt aatttctcag gatctctcgg agtttgccct gcatgtgcga gcctttctcg
    44941 gacttccggt tggcgggatc cgtcagtatg gtcctgcagc ttctgccgtt atcctgccac
    45001 aactgaccag tgagaatgtc acgtttgata atgtgcagaa tgccgtaggc gcaggtttgc
    45061 agattcgttt atttggtaag ccggaaattg atggcagccg tcgtctgggg gtggcactgg
    45121 ctactgcaga gagtgttgat gaagcgattg agcgcgcaaa gcacgccgca gcacagataa
    45181 aagtacaagc ttagccccgg cgaaaaacgc taccaaaatg cccgatcgag gatcgggcat
    45241 tttgactttt acagcttagc gccttctaca gcttcacgcg ccagcttagt aatgcggtcg
    45301 taatcgcccg cttccagcgc atctgccgga accagccagg aaccaccgat gcacagcacg
    45361 cttttcagcg ccaggtagtc acggtagtta gccggagaaa taccacctgt cgggcagaaa
    45421 cggacctggg agaacggacc cgcgatcgcc tgcagggctt tcacaccgcc gttagcttca
    45481 gccgggaaga atttgaactc tttcaaaccg tagtccatac ccagcatcag ttcggaaaca
    45541 gtgctgatcc ccggaatcag aggaatagtc ccttcggtag cagccttcag cagcggctcg
    45601 gtcagccccg ggctaattgc gaactgtgca cccgcttcgg tgacttctgc cagctgctgt
    45661 ggattcagca ccgtaccggc acccacaatc gcttcaggca cttctttggc gatagcacgg
    45721 atagcgtcaa ctgcacactc ggtacgcaga gtcacttcca gaacgcgcac cccaccagca
    45781 accaacgctt ttgccatcgg caccgcgtgt tccagttttt ttaccacgat aaccggtaca
    45841 accgggccgg tggtcaggat tgattctgca cttgttttcc agtttttcat cagagttttc
    45901 tctcgcctga ttacaaattt gtcgtcttaa aaagtgatac aggttgcgcc ctgttcggca
    45961 ccggacagtt tttcacgcaa ggcgctgaat aattcacgtc ctgttcccac gcgtgacgcg
    46021 ctcaggtcag gaatgtgcgg ttcgcgagca gccagttccg cttcgtctac cagcagcgtc
    46081 agttcgcctg tctgtccatt cacacgaatg atgtccccgt cgcgcacttt tgccagcagc
    46141 ccgccatcgt aggcttctgg tgttacgtgg atagctgacg gcactttacc tgaagcgccg
    46201 gagagtcgtc catcagtaac taacgcaatt ttgaaacacc ggtccaataa tacaccaagt
    46261 ggcggcatga gtttatgtaa ttctggcatt ccgttcgctt ttggcccctg atgacggaca
    46321 acaacgacac aatcgcggtc cagcaaaccc gcttcaaagg ccggcataac gtcatgctgg
    46381 ctttcaaaaa caaccgcagg cgcttcaatc acctggttct caaccggcac ggcagaggtt
    46441 ttcataaccg cacggcccag gttaccgctt aacacttttg tcccaccatg atgagagaaa
    46501 ggttgttcga aggaagcgat cacattgctg tcgagtgatt tttccgcccc ttcccgccag
    46561 tccagttcac cattattcag ccatggttca agggtataac gagacagacc aaagcctgcc
    46621 accgtattga catcttcatg cagcaggcct gctttgagca gttcacgcac cagaaccggt
    46681 acgccacctg ccgcctggaa gtggttaata tcggccggac cgttcgggta gagacgtgcc
    46741 atcagcggta caacatcaga aaggtcagag aagtcatccc agttaatctg aataccggcc
    46801 gcgcgtgcca tcgccaccag gtgcatggtg tggttagtgg aaccaccggt cgccagcagt
    46861 gcaacgatac cgttcactac cactttctca tctatcatct taccgatcgg catccattca
    46921 ttaccattac cggtcatgcg tgtaacctgg cgcgcagcgg ctgcggtcaa agcatcgcgc
    46981 agcggagaat ccggatgaac aaaagaagag cctggcaact gcatccccat aaactccacc
    47041 accatctggt tggtgttggc agtaccgtag aaagtacatg ttcccggcgc atgataagac
    47101 gcggcttctg actccagtaa tgccatgcgg tccactttac cttcggcata aagctggcga
    47161 atacgcactt tttctttatt tggcaaaccg cttgccatcg gtccagacgg cacaaacacc
    47221 gcaggcaaat gcccaaacga cagggctgcc atcgtcagac ccgggacaat cttgtcgcac
    47281 acaccgagga acagagcacc atcaaacatg ttatgggaca gccccaccgc cgcagacatc
    47341 gcaatcactt cgcggcttaa cagcgacaat tccattccat cctgcccctg ggtgacacca
    47401 tcacacatcg ccggaacacc gcctgcaacc tgaccaaccg catttgcttc atgcagggct
    47461 ttacgaatga tttctggata gtgttcataa ggctggtgcg cggagagcat gtcgttatag
    47521 gaggtgatga tggcgatatt gttacgcaac atgcttttca aagaggcttt gtcttctggc
    47581 tggcaggcag cgaaaccgtg tgccaggtta ccgcatgcca actgcgaacg atgaacggtc
    47641 gaagttttcg cttgttctat ccgggcgagg taagcagagc gagtctcgcg cgaacgttca
    47701 atgattcgat ttgttacgcg taacaattgt ggattcataa aggctcctga aattgagttg
    47761 tcagagcagg atgattcaca acgcgtttca ttcagaggat ttatgactga aacgcctgta
    47821 accggagctc atagggcaaa aacgtttcag gcagtgtaat aaaaaaagcc tcgtgggtga
    47881 atccgcacga ggcctgaaag tgtaaaaatt gttctacaat ctgtgcaaga tcatgttacc
    47941 ggtaaaataa ccataaagga tgagcgcaga tattactcaa actcattcca ggaacgacca
    48001 tcacgggtga tcatcgccac tgaggcaaca ggtccccagg ttccggcctg atacggtttc
    48061 ggcgcatcat tgtccatcgc ccacgcctca gtaatggagt ctacccattt ccaggcttct
    48121 tccacttcat cacgacgaac aaacagtgcc tgaataccac gcatggtttc cagcagcaga
    48181 cgttcatagg catccgccag atgcgtctga ttaaaggttt ctgaatagct caggtccagc
    48241 ttggtgattt gcaggttatg tttgtggtcc aggccaggaa ctttattcag tacctggata
    48301 tccacgcctt catcaggttg cagacggata gtcagtttat tttgcggtag atcctgccac
    48361 gactctttaa acagattcag ttcaggtgtt ttgaaataga ccacgacttc agagcatttg
    48421 gtcggcagac gtttaccagt acgcaggtag aatggcacac cggcccagcg ccagttatca
    48481 atgtcgacgc ggatcgccac gaaggtttct gtattgctgc tcttgttcgc gccctcttct
    48541 tccagatatc ccggcacttt tttgccctgg gcgaagcccg cagtatattg cccgcgtacg
    48601 gttttttcgc gtacgttgga gcggtcaata cggcgcagag acttcagtac tttcactttt
    48661 tcatcgcgga tgctgtctgc gctcaggtca gacggcggag acatcgcaat catgcagaga
    48721 atttgtagca ggtggttctg gatcatgtcg cgcatctgac cggctttatc aaaatagccc
    48781 cagcgccctt cgatccccac ttcttctgcc acggtaatct caacatgatc gatggtgcga
    48841 ttgtcccagt tgttcacaaa cagagagtta gcaaaacgca gcgccaacag gttcagcacc
    48901 gtttctttac caagatagtg gtcgatacgg taaacctggc actcctcgaa gtattcgcca
    48961 acctgatcgt tgatttcctg cgaggtcgcc agcgacgtcc ccagcggttt ctccatgact
    49021 acgcgtgccg gtttagcatt cagttttgcc tcgccaagcc ctttgcaaat tgcgccaaaa
    49081 gtgctgggcg gcatggcaaa gtagttaatg gtgatacgat ttttttgatc cagcatcgcg
    49141 ccgagacggc tgaatgcagc agtgtcattg acatcgagat tacaaaaatc cagacgtgca
    49201 ctcagggtgt cccataaacc ttcatcaatg gtttctttca tgaaagtttc gagcgcctcg
    49261 cggacaactt tggtataagc cgctttatcc cagtcagcac gccctacgcc gataatccgg
    49321 gtgtccgggt tgagctgacc ggctttttcc agttgataca gggaaggcag caatttacga
    49381 cgcgcaaggt cgcctttcgc gccgaaaatg accaggtcac aggcctgggc tgtttgcgtt
    49441 accgccatgt cattctcctt aagttaacta tcccggtact taagccaggg tatacttgta
    49501 attttcttac ggtgcactgt actgctttta cgagcttgcg aaaactgtaa acgcttatcc
    49561 acccgtgcga ttacggtaaa agcgcgcaaa gtgcggcaaa actgataaaa aaatcatcgt
    49621 tttccattgg gtaaaaatct gacactgatc atgttatgaa aaaaaataac aactttttta
    49681 tctgcttttg tcattaacac agcacacagg cgtaatatct gacaaaactg catttcgatt
    49741 tctttcagtg cggaaatcgt cattacccgt gagtctcttt acatcatgaa tatgctggaa
    49801 aaaatccagt ctcagctgga acatttgagc aaatcagagc gcaaagttgc cgaggtcatt
    49861 ctggcttcgc ccgataacgc gatccattcg agtattgctg ctctggcgct ggaagccaat
    49921 gttagcgaac cgacggtgaa tcgtttctgt cgcagcatgg acacgcgcgg ttttcctgat
    49981 tttaaacttc atctggcaca aagtctggcg aatggcactc cctatgttaa tcgcaacgtc
    50041 aatgaagatg acagcgttga atcatacacg gggaaaatat ttgagtccgc aatggcaacg
    50101 ctcgatcatg tccgtcattc attggataaa tctgccatca accgcgccgt cgacttgctc
    50161 actcaggcaa aaaaaatcgc ctttttcgga ttaggctctt cagccgccgt tgcccacgat
    50221 gcgatgaata agttctttcg ttttaatgtt ccggttgtgt actccgatga tattgtgctg
    50281 caacgcatga gttgtatgaa ttgtagcgac ggagacgtgg tggtgctgat ttctcacacg
    50341 ggaagaacaa aaaatctggt cgagctggcg cagctggcac gcgaaaacga cgccatggtg
    50401 attgccctca cctctgcggg taccccgctc gcccgggaag caacgctggc aattaccctc
    50461 gacgtaccgg aagatactga catttatatg cccatggttt ctcgacttgc acagctgacc
    50521 gtgatagatg tgctggcgac aggatttact ttgcgacgcg gtgcaaaatt cagagataac
    50581 ttgaagcggg tcaaggaagc gctgaaggaa tcgcgttttg ataagcagtt acttaattta
    50641 agtgacgatc gctaaaagcg actgtcactg ttctaatctt atacgacatc cgaatgagat
    50701 taatttatcg ccatcgcggc gttatttcat tcggatttca tgttcaagca acacctggtt
    50761 gtttcagtca acggagtatt acatgtccag aaggcttcgc agaacaaaaa tcgttaccac
    50821 gttaggccca gcaacagatc gcgataataa tcttgaaaaa gttatcgcgg cgggtgccaa
    50881 cgttgtacgt atgaactttt ctcacggctc gcctgaagat cacaaaatgc gcgcggataa
    50941 agttcgtgag attgccgcaa aactggggcg tcatgtggct attctgggtg acctccaggg
    51001 gcccaaaatc cgtgtatcca cctttaaaga aggcaaagtt ttcctcaata ttggggataa
    51061 attcctgctc gacgccaacc tgggtaaagg tgaaggcgac aaagaaaaag tcggtatcga
    51121 ctacaaaggc ctgcctgctg acgtcgtgcc tggtgacatc ctgctgctgg acgatggtcg
    51181 cgtccagtta aaagtactgg aagttcaggg catgaaagtg ttcaccgaag tgaccgtcgg
    51241 tggtcccctc tccaacaata aaggtatcaa caaacttggc ggcggtttgt cggctgaagc
    51301 gctgaccgaa aaagacaaag cagacattaa gactgcagcg ttaattggcg tagattacct
    51361 ggctgtatcc ttcccacgct gcggcgaaga tctgaactat gcccgtcggc tggcacgcga
    51421 tgcaggatgt gatgcgaaaa ttgttgccaa ggttgaacgt gcggaagccg tatgcagcca
    51481 ggatgcaatg gatgacatca tcctcgcctc tgacgtggta atggttgcac gcggcgacct
    51541 tggtgtggaa attggcgatc cggaactggt cggcattcag aaagcgttga tccgtcgtgc
    51601 gcgtcagctt aaccgcgcag taatcacggc gacccagatg atggagtcaa tgattactaa
    51661 cccgatgccg acgcgtgcag aagtcatgga cgtagcaaac gccgttctgg atggtactga
    51721 tgctgtgatg ctttctgcag aaactgctgc tggtcagtat ccttcagaaa ctgttgcagc
    51781 catggcgcgc gtttgcctgg gtgcggaaaa aatcccgagc atcaacgttt ctaaacaccg
    51841 tctggacgtg cagttcgaca atgtggaaga agctattgcc atgtcagcaa tgtacgcggc
    51901 taaccacctg aaaggcgtta cggcgatcat caccatgacc gaatcgggtc gtacggcgct
    51961 gatgacctcc cgtatcagct ctggtctgcc gattttcgcc atgtcacgcc atgaacgtac
    52021 gctgaacctg actgctctct atcgcggcgt tacgccggtg cacttcgata gcgctaatga
    52081 cggcgtagca gctgccagcg aagcggttaa tctgctgcgc gataaaggtt acttgatgtc
    52141 tggtgacctg gtgattgtca cccagggcga cgtgatgagt accgtgggtt ctactaatac
    52201 cacgcgtatt ttaacggtag agtaagtacg ttgccggatg cggcgaaaac gcctcatccg
    52261 gcctacagtt caatgatagt tcaacagatt tcgaatcttc tgaagcaaac ttgaacttat
    52321 cacacggcgg aagcctctcc tcgcgagagg ctttttttat ttgatgggat aaagatcttt
    52381 gcgcttatag ggttggattt cgcccggttt gcgagttttc agcaatttta atatccaggt
    52441 gtattgttct ggtcgcggac caacaaaaat ctcgacttct tcattcatcc gccgcgcaat
    52501 cgtatggtca tccgcctcta acagatcatc catcggtggg cgcacctgaa tcgtcaaacg
    52561 atgcgttttg ccatcataaa tcggaaacag cggtacaacg cgcgcacggc acactttcat
    52621 caaacgacca atcgcgggca acgtcgcttt ataggtggca aagaaatcaa caaattcgct
    52681 gtgctctggg ccatgatcct gatcgggtaa ataatatccc cagtacccct gacgtaccga
    52741 ctggatgaat ggtttaatac catcatttct cgcatgcaga cgaccgccaa aacgacgacg
    52801 caccgtgttc cagacataat caaaaaccgg gttgccctga ttatggaaca tcgctgccat
    52861 tttctgccct tgcgaggcca tcagcatggc cgggatatcg acggcccaac cgtgcggcac
    52921 cagaaaaatc actttctcgt tattacgccg catctcttcg atgatctcca gcccttgcca
    52981 gtcaacgcgc ggctgaattt tctccggccc gcgtattgcc aactcagcca tcattgccat
    53041 cgcttgcggc gcggtggcaa acatctcatc aacaatcgct tcgcgttcag cttcactacg
    53101 ttctggaaag cagagcgaca aattgattaa cgcacgacgg cgtgagcttt ttcccagtcg
    53161 tccggcaaaa cgtcccagcc gtgccagaat gggatcacgg aactttggcg gcgttaaagc
    53221 gatacccgcc atcgctgcta cgcccagcca tgctccccag tagcgcgggt ggcgaaagga
    53281 tttatcaaac tcaggaatgt attcgctatt attttttttc gtttccatgc ttttccagtt
    53341 tcggataagg caaaaatcaa tctggtgata gtgtagcggc gcaacttgcc ccgcaccaaa
    53401 taaaaaagcc ggtactgact gcgtaccggc tgcgaatgga tgttaattaa tcaaaccgta
    53461 gctgcggcac aatctctttg gcctgtgcca ggaattcgcg acgatcggag ccggtcagcc
    53521 cttcggtacg cggcagtttt gccgtcagcg ggtttacggc ctgctggttt atccatactt
    53581 catagtgcag atgcggcccg gttgaacgtc cagtattacc ggaaagcgcg atacggtcgc
    53641 cacgtttcac cttctgtccc ggtttcacca ggatcttgcg caagtgcata taacgcgtgg
    53701 tgtagctgcg accatgacga atagccacat aataacctgc tgcgccactg cgtttggcaa
    53761 ccaccacttc accgtcaccc actgaaagca ccggcgtacc ctgtggcatg gcgaaatcaa
    53821 cacctctgtg tggcgcaaca cgaccagtca ccggattagt acgacgcggg ttaaagttag
    53881 acgagatacg gaactgtttc gccgtcggga atcgcaagaa tcctttcgcc agaccagtac
    53941 cgttacgatc gtagaatttg ccatcttcag cgcggattgc gtaataatct ttaccttctg
    54001 aacgcaaacg tacgcccagc agttggcttt gctcacgttt accatcaagc atttctcgtg
    54061 acattaacac cgcaaattca tcgccttttt tcagtttgcg gaaatccatt tgccactgca
    54121 tggctttaat cactgcgctc acttcggcgc tggttaaacc ggcgtttctg gcgctggcaa
    54181 caaagcttcc cccgacggta cctttcagca gattgttgac ccactctcct tgctgcattt
    54241 cgctggtcat tttaaaaccg ttagcggcag tacggtcata ggttcgggtt tcacgacgag
    54301 acacttccca ggtgaggcgc tgcagttcgc cgtccgcggt taatgtccag gagagttgtt
    54361 gaccgatttt caggttacgc aattctttgt cggcagcagc cagttgggtg atatcaccca
    54421 tatcaatacc atactgattg agaatgctgc ttagcgtatc gccagtggaa acaacatatt
    54481 catgcacgcc cgcttcaccg gcgattttgt catccagttc atcctgggga atggcttcat
    54541 cttcttgtgc agcttgatca atcggctcac tggcttcagg taagagcgaa cgaatttcgt
    54601 tctgttccag ctcaatggtt ttgacaattg gcgtggcatc gcggtgataa acatagggcc
    54661 gccagacagc gacggccaga gtaagaacgg tgagcgaccc caacataacg cggtgtggtc
    54721 gcggtaaatt attaaacgcc agggcgacag agcgggctat ctgttgcacg taatcacttc
    54781 ctcattaatc tcctttcagg cagctcgcat actggttggc taattgattc aggaattctg
    54841 aatagcttgt tttacccagt ttgatattcg tccccagggg atccaacgtt cccatacgaa
    54901 cggatgtccc tcgtgcgacg ctctcaacga ccgctggcct gaactgtggc tcagcaaaaa
    54961 cgcaggttgc tttttgctca accaactgtg ttcttatttc atgtaaacgc tgcgcgccag
    55021 gttgaatctc agggttaacg gtaaaatgac caagcggtgt cagtccgaac tgtttttcga
    55081 aatagccgta agcatcgtga aaaacgaaat aacctttccc cttgagcggc gcgagctcgt
    55141 taccaacctg cttttcggtt gaggctaatt gtgcctcaaa atccttcaag ttggcgtcaa
    55201 gtttggctcg actttgcggc ataagttcca ctaattttcc atggattgca accgctgtag
    55261 cccgcgctat ctctggggaa agccaaagat gcatgttgaa atcgccgtga tggtgatctt
    55321 cgtcactttt ttccgcgtgg tcgtgatcat catcatcgcc gtgaatactt ttcatcagca
    55381 gcggtttcac attctctagc tgcgcaatcg ttacctgttt cgcttcaggt aatttactta
    55441 ccggtttttg catgaacgct tccatctccg ggccaaccca aacgactaag tccgcgttct
    55501 gtaagcgttt tacatctgat ggacgcagtg aataatcatg ttctgaagcg ccgtcaggta
    55561 gtaaaacctc cgtttctgtt accccatcag caatggcaga agcgatgaac ccaacgggtt
    55621 taagcgaagc gacaacggca gcatctgcgg cctgtgttgc accgccccag agagcggcgg
    55681 ataatgctgc gaaaagaagc gtttttttat gtaacataat gcgaccaatc atcgtaatga
    55741 atatgagaag tgtgatatta taacatttca tgactactgc aagactaaaa ttaacatgac
    55801 aagtctggtt tccctggaaa atgtctcggt ttcttttggc caacgccgcg tcctctctga
    55861 tgtgtcgctg gaacttaaac ctggaaaaat tttgacttta cttgggccaa atggcgcagg
    55921 taagtcgaca ctggtacggg tagtgctcgg gctggtaaca cccgatgaag gggttatcaa
    55981 gcgcaacgga aaactgcgca tcggctatgt accgcagaag ctgtatctcg acaccacgtt
    56041 gccactgacc gtaaaccgtt ttttacgctt acgccctgac acacataaag aagatatttt
    56101 gcctgcactg aaacgtgtcc aggccgggca tctgattaac gcaccgatgc aaaagctctc
    56161 gggtggcgaa acacagcgtg tattgttagc gcgagcattg ttaaatcgac cgcaattatt
    56221 agtgctggat gaacccactc agggcgtgga tgtgaatggt caggtggcgt tatatgacct
    56281 tattgaccag ttgcgtcgcg aactggattg tggcgtttta atggtctctc acgatctgca
    56341 tctggtaatg gcaaaaaccg atgaagtgct ttgcctgaat caccacattt gttgttccgg
    56401 tacaccggaa gttgtttccc tgcatccgga gtttatttct atgtttggtc ctcgtggtgc
    56461 tgaacaactg ggtatctatc gccatcatca taatcatcgt cacgatttac agggacgaat
    56521 tgttttgcgt cggggaaatg atcgctcatg attgaattat tatttcccgg ttggttagcc
    56581 gggatcatgc tcgcctgtgc cgcgggtccg ctgggttcgt ttgtggtctg gcgtcgtatg
    56641 tcttatttcg gtgatacgct ggctcatgcc tcattacttg gcgtcgcgtt tggtttgttg
    56701 ctggatgtga atccattcta tgcggtgatt gcagttacgc tgctgctggc gggcggtctg
    56761 gtatggctgg agaagcgtcc acagctggcg atcgacacgt tattagggat tatggcgcac
    56821 agtgccctgt cgctgggcct ggtggtcgtt agtctgatgt ctaacattcg tgttgatttg
    56881 atggcttacc tgttcggtga tttgctggca gtgacgccag aagatctcat ctctattgcg
    56941 attggcgtgg tcatcgtgtt ggcgattctg ttctggcaat ggcgcaattt gctgtcaatg
    57001 acgattagcc cggatctggc gtttgttgat ggtgtgaaat tacagcgcgt gaaattgttg
    57061 ttgatgctgg tgacagcatt gacaattggt gtagcgatga aatttgtcgg cgcgttgatt
    57121 attacttcgc tgctgattat tcctgcagct acagcgcgtc gctttgcccg cacgccggaa
    57181 cagatggccg gtgtcgctgt tttggtgggg atggtggcag tgactggcgg tttaaccttt
    57241 tccgcatttt acgatacgcc ggcgggtccg tcggtggtgc tatgtgcggc actgttattt
    57301 attatcagta tgatgaaaaa gcaggccagc taatctatcg ctgaacacat ttgtcggatg
    57361 cggcgcgagc gccttatccg acctatggtt cgctatctct ggtatatgcc tgataagacg
    57421 cgaatagcgg cgcatcaggc aacactgcca gtgtcggatg cgccgcgagc gaccgatccg
    57481 acttacggca tttctggcgg cgtgatgccg aagtggttcc acgcccgcac tgtcgccata
    57541 cgcccgcgcg gtgtacgctg caaaaagcct tgctgaatca aataaggttc cagcacatcc
    57601 tcaatggttt cacgttcttc gccaatggca gccgccaggt tatccagacc taccggccca
    57661 ccaaagaact tatcgattac cgccagcaac aatttgcggt ccatataatc gaaaccttcg
    57721 gcatcgacat tcagcatatc cagcgcctga gcagcgatat cagccgagat ggtgccatcg
    57781 tgcttcactt cggcgaaatc acgcactcga cgcagcagac ggttagcaat gcgcggcgta
    57841 ccgcgagcgc gacgagcaac ttccagcgcg ccgtcatcac tcatctcaag ccccataaag
    57901 cgtgcgctgc gactgacgat atattgcaga tccggcacct gataaaactc cagacgttgc
    57961 acaataccaa aacgatcgcg taacggtgat gtcagcgaac ctgcgcgcgt ggttgcgcca
    58021 atcagtgtaa acggcggcaa atcaatttta atggagcgtg ccgccggacc ttcaccaatc
    58081 atgatatcca gttggtagtc ttccattgcc gggtacagca cttcttcaac aactggcgat
    58141 agacggtgga tctcatcaat aaacaacacg tcatgcggtt caaggttagt gagcatcgct
    58201 gccagatccc ccgccttttc cagcaccgga ccagaagtcg tgcgtaaatt aacgcccatt
    58261 tcattggcga caatgttagc aagcgtagtt ttacccaacc ccggaggacc aaaaatcaac
    58321 agatgatcga gggcatcgcc gcgcagtttc gctgctttga tgaaaatctc catctgtgaa
    58381 cgaacctgcg gctgaccaac atactcttcc agtaatttag ggcgaatggc gcgatctgcc
    58441 acatcttccg gcaaagtggt accggcagaa atcagacggt ctgcttcaat catcctttac
    58501 ctcataacgc ggcgcgtagg gcttcgcgaa ttaatgtttc actgctggcg tcagggcgag
    58561 cgattttgct caccatgcgg cttgcttctt gtggtttata gcccagcgcc accagcgcgg
    58621 caaccgcttc ttgttcagca tcgtcggtcg ccgggctggc aggagacgtg agtaccaggt
    58681 cggcggctgg cgtaaagaga tcgccatgca aacctttaaa tcggtctttc atttcaacaa
    58741 tcaagcgttc ggcggttttt ttaccaatac ccggcagttt caccagtgcc cccacttctt
    58801 cacgctcaac ggcattaacg aactgctgcg ctgacattcc agagaggatc gccagcgcca
    58861 acttcgggcc gacgccgttg gttttgatca actctttgaa caacgtgcgc tcttgtttat
    58921 tgttaaaacc gtacagcagt tgcgcgtctt cacgcaccac aaagtgggtg aaaacgatcg
    58981 cttcctgacc cgcttcaggg agttcataaa aacaggtcat cggcatatgc acttcatagc
    59041 ctacgccgcc cacttcaatt aacaccagcg ggggttgttt ttcaatgatg atgcctctga
    59101 gtctgcctat cacatgacgc tcctgcgtaa tgaatcaaag ataatgctgt atgataaaaa
    59161 aatgctggat agatatccag cgaaggatga agaaaacttg cgaggcgtcc cgatgttctg
    59221 aaaaatgacg cagtataatt ttttctacag gttatattgg aagcaaatat ttaaatatta
    59281 catattcagt gaagaaatgc gtaataaaaa tacacattgc gcctcctgaa aaaataaatt
    59341 ttttatgcta ttacgtatat tcatatctat ttcaatggaa tgacaacatg aatattaatt
    59401 accctgctga atatgaaatt ggtgatatcg tctttacatg tataggtact gccttatttg
    59461 gccaaatatc agctgcatca aattgctgga gtaatcacgt tgggatcatt accggtcata
    59521 acggtgaaga cttcctggtt gcagaaagcc gggttcccct ttcaactatc accacgctat
    59581 ctcgttttat taaacgttct tctaatcaac gctatgctat aaagcgatta gacgccgggc
    59641 ttacagaaca acaaaaacaa cgaattgttg aacaggttcc ctcccggcta cgcaaacttt
    59701 accacaccgg ttttaaatac gaatcttcgc gccagttctg ttcaaaattt gtttttgata
    59761 tttataaaga ggcgctatgt attccggtgg gtgaaataga gacgtttgga gaactgttaa
    59821 atagcaatcc gaatgcaaaa ctcactttct ggaaattctg gttcctgggt tctattccgt
    59881 gggagcgtaa aaccgtcacg ccagccagtt tgtggcatca tccgggtttg gtgttgattc
    59941 atgcggaggg agtggaaacg cctcagccgg aactgaccga ggcggtataa cttaacgcag
    60001 tcgccctctc gccaggttca gccgcgattc gctcatctgc atcgcattct gactgacgtg
    60061 acaatgggta atcgcgatag ctaatgcatc ggcagcatcc gcctgtggat tagcgggcag
    60121 tttcagcaag gtgcggacca tatgctgcac ctggcttttt tcagcactac caatacctac
    60181 caccgtttgc tttacctgac gtgccgcata ttcaaatacc ggcaattcct gattcaccgc
    60241 cgccacaatc gccacgccgc gcgcctgacc cagtttcagg gctgagtcag cgttcttcgc
    60301 cataaagact tgttcaatgg cgaaataatc aggctggaac tgggtaatga tttccgtcac
    60361 gcccgcatag atgagcttca gacgagacgg taaatcatcc actttggtgc gtatgcatcc
    60421 gctacccagg taggagagtt gcctgcctac ctggcggatg acgccatagc cggtcacgcg
    60481 cgaacccggg tcaatgccga gaataatagc catcacgcgt ctccgttttg ctgtttagca
    60541 ggccccatca gagagtcgct gcaacctcat cagagatttc accgttatgg tagacttcct
    60601 gcacgtcgtc gcaatcttcc agcatatcga tcagacgcat cagtttcggt gcggtttctg
    60661 catccatatc agctttggtg gacgggatca tggaaacttc cgcgctgtct gctttcagac
    60721 ctgccgcttc cagagcgtcg cgtactttgc ccatttcttc ccatgcagtg tagacatcaa
    60781 tcgcgccgtc atcataggtc acaacgtctt cagcacctgc ttccagcgct gcttccatga
    60841 tggtgtcttc atcgcctttc tcgaaggaga tcacgccttt tttgctgaac agataagcta
    60901 cggaaccatc agtaccgagg ttaccgccac atttactgaa tgcatggcgc acttcagcaa
    60961 cggtacggtt gcggttgtca gacagacatt caatcatgat tgccgtgccg ccaggaccgt
    61021 aaccttcgta gatgatggtt tccatgtttg catcatcatc accgcccaca cctcgtgcaa
    61081 ttgcgcggtt cagagtgtca cgagtcatgt tgttagacag tgctttatca attgctgcac
    61141 gcagacgcgg gttagcgtcc ggatcgccac cgccgagctt agctgcagtt accagctcac
    61201 gaatgatttt agtgaagatt ttaccgcgct tagcatcctg cgcagcttta cgatgtctgg
    61261 tgttggccca tttactatga cctgccataa aaatatctcc agatagcctt gcctgttcag
    61321 gcagcgttaa ttacaaactg ttcaatcgcc tgccggttgc tccaggactt agtgagcgcc
    61381 gccgcagcag gcgcatcaag ccacttgtag gccagatgtt cagtgaagac gacctggcgt
    61441 tcgtgcggga gcgcaagaca gaaccatgat tccgtattac gcgtcacgcc cggcgcatag
    61501 cgatgacgta aatgtgaaaa aatttcaaat tctaccgtgc gctgacagtc aattaaggtc
    61561 agttgttcag cgacaacatc gatggtgacc tcttccttta cttcgcgcat ggcagcttgc
    61621 ggcgcggttt caccctcttc cacgctgccg gttaccgact gccagaaatc gggatcgtca
    61681 cgccgctgca acatcagcac ccgtttcgta tcttgcgcat agatgaccac taagatcgaa
    61741 acgggacgct tatacacttt atccttcacg ctgcctcttc gttgactgcc ttcgctcatc
    61801 ccattcacat agttatctat gctcatggga gttcactcag ttgccgcctc gatgcaacgc
    61861 gaatgatttc gtgtatttga gtcatatcag ttattctctg ccttcttcac aacctgaatg
    61921 ctcagctcgg ccagtgcagt cgggttagca aagctcggtg cttcagtcat cagacacgcc
    61981 gccgccgtgg ttttcgggaa ggcgataacg tcacggatat tgtcggtgcc ggtcagcagc
    62041 atcgtcagac ggtcaagacc gaatgccaga cctgcgtgcg gcggagtacc gtatttcagg
    62101 gcgtcgagca ggaagccgaa tttctcgcgc tgttcctctt cgttgatacc cagaatacca
    62161 aacaccgtct gctgcatatc accattatgg atacgcacag aaccaccgcc cacttcgtaa
    62221 ccattgatga ccatatcgta agcgttcgcc accgcatttt ctggtgcagc tttcagttct
    62281 gccgccgtca tgtctttcgg tgaggtgaac ggatggtgca ttgccgtcag gccgccttca
    62341 ccgtcgtctt caaacatcgg gaaatcgata acccacagcg gtgcccattt gctttcgtcg
    62401 gtcagaccaa ggtctttacc cactttcagg cgcagtgcgc ccatcgcgtc ggcaacaatt
    62461 ttcttattgt cggcaccgaa gaaaatcata tcgccatctt gcgcggcagt acgatccagg
    62521 atggcttcga tgatttctgc attaaggaac ttcgctaccg ggctgttgat accttccaga
    62581 cctttcgcgc gttcgttaac tttgatgtac gccaaacctt tcgcgccgta gattttaacg
    62641 aagttaccgt attcgtcgat ctgtttacgg gtcagcgatg cgccacccgg aacacgcaga
    62701 gcggcaacac ggcctttcgg atcgttcgcc ggacctgcaa atactgcaaa ctcaacagat
    62761 ttcagcagat cggcaacgtc ggtcagttcc atcgggttac gcagatccgg tttatcagaa
    62821 ccataacggc gttctgcttc tgcaaaggtc attaccggga aatcgcccag atccacgccc
    62881 ttcacttcca gccacagatg acgcaccagc gcttccatca cttcacgcac ttgcggcgcg
    62941 gtcatgaaag aagtttccac atcgatctga gtaaattcag gctgacgatc agcacgaaga
    63001 tcttcgtcac ggaagcattt aacgatctga tagtaacggt caaagccgga catcatcagc
    63061 agctgtttga acaactgcgg ggattgcggc agcgcgtaga atttaccttt gtgcacacga
    63121 gaaggcacca ggtagtcacg cgcgccttcc ggcgtggctt tggtcagcat cggggtttcg
    63181 atgtcgagga aaccgtggtc atccataaaa cggcgcacca ggctggtgat tttagcgcgg
    63241 gttttcaggc gctgagccat ttccgggcga cgcaggtcga ggtagcggta tttcagacgc
    63301 gcttcttcgg tgttgacgtg gttagagtca agcggcagaa catctgcgcg gttgatgata
    63361 gtcagcgagg acgccagtac ttcgatttcg ccagtcgcca tatcgcggtt aatatttttt
    63421 tcgtcacgcg cacgtacggt gcccgtgacc tgaatgcaga actcattacg cagttcagag
    63481 gccagcttta acgcgtccgc acgatccgga tcgaaaaata cctgcacgat accttcgcgg
    63541 tcgcgcatat cgatgaagat caggctacca agatcacgac gacggttgac ccaaccacac
    63601 agagtcacct gctgccccac gtgggacaaa cggagctgtc cacaatattc tgtacgcatg
    63661 agatatccct taacttagct gccggcggat gccccctgct gcgcaggtga ccaagtcgca
    63721 gcgttagctg tatgtcacaa ctgaatgaaa aaaggcggct attatactgg aaattctgcc
    63781 gcaccgtaag agcctggccc gcgctggaac acctcgttac cactttatat cgggcctgaa
    63841 atcagactct acgccagttt gctataaagg tgttgcccga actcataaaa attaacaaaa
    63901 tttgtcgttc cgccatcggc taatcgcatt aaggtgagag gcacgatttt gttttgtcag
    63961 gagtcatcat gcttgaactt aatgctaaaa ccactgcgct ggtggtgatt gatttacaag
    64021 aaggcatctt accttttgcc ggaggtccac atactgccga tgaggtggtt aatcgcgccg
    64081 ggaagctggc ggcgaaattt cgcgcgagcg gtcagcccgt gtttctggtg cgcgttggct
    64141 ggtctgccga ttatgccgaa gcattaaaac agccggttga tgtcccctcc cccgctaaag
    64201 tgttacccga aaactggtgg caacatcctg ctgcattagg tgcaaccgac agcgatatcg
    64261 aaatcatcaa acgtcaatgg ggtgcgtttt acggtacgga tctggagttg caattacgcc
    64321 gccggggtat cgatacaata gtattatgtg ggatctcgac caatatcggt gttgaatcca
    64381 ccgcccgcaa tgcctgggaa ctcggtttta atctggtgat tgccgaagac gcctgcagcg
    64441 ccgccagcgc cgagcagcac aataacagca tcaaccatat ctacccacgc atcgcccgtg
    64501 tgcgcagtgt ggaagagatc ctcaacgcat tatgatttac atcggtctgc cgcaatggtc
    64561 gcatcctaaa tgggtgcggt tggggatcac cagccttgaa gagtatgccc gccactttaa
    64621 ctgtgtggag ggcaacacca cgctttacgc cctgccgaaa tccgaggttg tcctgcgctg
    64681 gcgtgagcag accacagata attttcgctt ctgttttaag tttccggcga ccatttcgca
    64741 tcaggcagca ttacggcatt gcgatgattt agtgactgaa tttttgaccc gcatgtcacc
    64801 gttagcgccg cgcattgggc aatactggct gcaactgcct gccacatttg gcccacggga
    64861 tctgcctgcg ctttggcatt ttctcgattc ccttcccggc gaatttaatt atggtgtgga
    64921 agtccgccat ccacagtttt tcgccaaagg ggaagaggaa caaacgctta atcgcggttt
    64981 acatcagcgc ggcgttaatc gggtgatttt agacagccgc ccggttcatg cagcacgtcc
    65041 acacagtgaa gctattcgcg acgctcaacg aaaaaaacct aaagttccgg tacatgctgt
    65101 actgacggcg acaaatccgc tgatccgttt tatcggtagt gatgatatga cgcaaaaccg
    65161 ggaattattt caggtctggt tacaaaaatt agcgcagtgg catcagacca ctacgcctta
    65221 tcttttttta catacgccag atattgccca ggccccggaa ctggtacata ccctgtggga
    65281 agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac agcaatcttc
    65341 tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta aagggagttt
    65401 gtatggtaag cgcgctgtat gccgttttaa gtgcgttgtt attaatgaag ttctcttttg
    65461 atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg cgacggcggt tttagcgaac
    65521 tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc gcgattgtgt
    65581 taatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt tgcggcatcg
    65641 ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg ttccgctggc
    65701 gacgttccgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg gcgaatcttt
    65761 ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc cactttcttt
    65821 ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc tatcgccaga
    65881 ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat gatccagcgt
    65941 tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga gcgcttcgtt
    66001 caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac gctctcggtg
    66061 cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgacaactc tccggcgatg
    66121 attgaacgct gccgtcgtca tattgacgcc tataaagccc ctacgccagt agacgttatt
    66181 gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct gaattttacc
    66241 ctgcaattcc tggaaccttc cgagcgtcag gcgttactgg ataaaattta tcaagggctg
    66301 aacccgggcg gtgcgctggt gctttcggaa aaattcagtt tcgaagatgc caaagttggt
    66361 gaattgctgt tcaacatgca ccacgacttt aaacgtgcca acggttacag cgaactggag
    66421 atcagccaga agcgcagcat gctggaaaac gtgatgctaa ccgattctgt ggaaacccat
    66481 aaagcacgcc tgcatcaagc tggttttgag catagcgagc tgtggttcca gtgctttaac
    66541 tttggttccc tggtggcatt aaaagcagag gacgctgcat gatcgacttt ggtaactttt
    66601 attctctgat tgccaaaaat catctttcac actggctgga aacgctgccc gcgcagattg
    66661 ctaactggca gcgcgagcag cagcacgggc tgtttaagca gtggtccaac gcggtggaat
    66721 ttctgcctga aattaaaccg tatcgtctgg atttattgca tagcgtgacg gcagaaagcg
    66781 aagagccact gagcgccggg caaattaaac gtattgaaac gctgatgcgc aacctgatgc
    66841 cgtggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa tggcgttccg
    66901 actggaaatg ggatcgcgtt ctgccccatc tttctgattt aaccgggcgc accattcttg
    66961 atgtcggctg tggcagcggt tatcacatgt ggcgcatgat tggcgcaggc gcgcacctcg
    67021 cggtgggaat cgaccctacg caactgtttc tgtgccagtt tgaagcggtg cgtaaactac
    67081 tgggtaacga tcagcgcgca catttgttac cgttaggcat tgaacagctt ccggcactga
    67141 aagcctttga taccgtcttt tcgatgggcg tgctttatca tcgtcgttca ccgctggagc
    67201 atctctggca gttgaaagat caactggtca atgaaggtga actggtgctt gaaacgctgg
    67261 ttattgatgg tgatgagaac acggtgctgg tgccgggcga tcgttacgcg caaatgcgta
    67321 atgtctattt catcccttcc gcgctggcgc tgaaaaactg gctgaagaag tgtggttttg
    67381 ttgatattcg cattgcggat gtgagcgtta ccactacaga agagcagcga cgcactgaat
    67441 ggatggttac cgagtctctg tccgatttcc tcgacccaca tgatccgagt aagacagttg
    67501 aaggttatcc ggcacctaaa cgcgcggtgc tgattgcgcg caagccgtaa agattagcgc
    67561 ccgtttccgg gcgcttttca cgtcttatgc ctgaacagct ggctgatcaa acgccgttaa
    67621 cttcggtgca ttgcccgtat atttttcgat atacaccagc gcagagttac cggcacaggc
    67681 atttgccagc tgagagctgg ggatatccgc cgttaacacg ttcgcactgc cgtttttaca
    67741 caagccattt tccagatctg gccatgcacc ttcatgcacg cataccacgc cttttttgat
    67801 cccgtcagtc accaccgcgc ctgtcagaat ctgaccacgt ctgttccaga cacgcaccag
    67861 atcgccattc gcaataccaa agcgagcagc atcttcggtg tgaatagtga tcggttcacg
    67921 atcggcaacc gcatattttt tacgcagttc cgcatagtta agctgactgt gtaaacggtg
    67981 tgccggatgt gcggtcagaa gctgcaactg cttctcgtcg gcggaacctt tccactcatc
    68041 aggcgcaagc caggttgggt gtgccgggca atccttatag ccaaattttt ccagcgtttt
    68101 ggagtaaatc tcaattttgc cgcttggcgt acccagcgca tttttcaccg gatcggcgcg
    68161 gaaatcacca taacgaacgt actgttcgtt cttctcgctg cggcgcattt cgatcagttt
    68221 attttgctgc cagaaagcat taaacatcgg catagtcacg cgttgcgcac gcgcaccttt
    68281 ctgagcggca tcatagaaaa atttcagcca cgccatttca tctctacctt cggtatagat
    68341 ctcttttccg ccaggtttga gtaattcagc aagatcggca aacacgtcaa aatcgttacg
    68401 cgcttcaaat tgcgcagcca cagcctgctt catcggcaca atatgctggt tgctgtaatc
    68461 accggtcatc gttaagtcat tgcgctcaaa cgatgtggta atcggtaata cgatatctgc
    68521 atgtttagca gctgcggtcc agtagcattc agaaacgacg atcatctccg gtttctgcca
    68581 tgctttaatc agacgattag tatcctggtg atgggtaaag ttaccgccac ccgcccacca
    68641 gatcattttg atattcggat acgtttgttc tttaccgtta tgctgatatt tgccgccagg
    68701 attttccagc gcatcgacga tacgggcaac aggaatcgcc gtcattccgc catcatcagc
    68761 ggcctcactg gcctgtccgg caatcgccgc agacatttcc ggcagcacgc cgccaacacg
    68821 cgtcgggttg ccaccgtttg agtaatgata agagaaacca aacccgccgc ccggtgtacc
    68881 aatctgccct aacatcgccg ccagcgtcac cagcatccag tgtttctgtt caccatattg
    68941 ctggcgctga attccccagc cagccatcag cattgtgcga ttcgccgcca tcagttcagc
    69001 gagtttgatt atctgcgctt ccggtacgcc agtaatttcc gccgcccagg ccgcgctctt
    69061 cggggtatta tcgctcttac ctgtcagata ctcttcaaac tgcggatacc cggtggtgta
    69121 tttttcaagg aacactttat cgtgtttgcc ttgtgtcatc agggtatgcg caatccctaa
    69181 catcagtgcc acgtcggtgc ccatattcgg cgcgatccag gtggcattat cgccaaagaa
    69241 ttcgatggtt tcggagcgga taggatcaat ggcaatcacg ggtttgccag attttttcag
    69301 ctgatggaag tattccagcc cttgctcatc ggtactgctc caggcaattt tcaaggtatt
    69361 cagcgggttc atcccccaca gcaccacaac ctggctgttt tccagaatca gcggccagga
    69421 ggtctgctgt tcatacacct ctacagaacc gaccacatgc ggcatgatca cctgtgctgc
    69481 cccggttgaa taatcgccgc tatgcccgga ataaccgccc gccaggttca tataacgttg
    69541 cagtaaggtt tgcgctttat gcaacacgcc agaagatcgc cagccgtaag atccggcaaa
    69601 aatggccgat ggtccgttag ctttacgaat acgatcatgt tgctcatgaa tcagttttaa
    69661 tgcttgttcc cagctcacct gtacataggt atcttcgcca cgaccttttg ccggttgcag
    69721 tggattatcg agatagcttt ttctcaccat cggatgctga atacgcgccg tggtgtgtac
    69781 ctgatccgcc gccgtagact gtaaggagtt cggtatggtt ttcgccagcg cgcctgtcga
    69841 agaaacaatc ttgccgtcct tcacttctac gttcatcgct ccccaacgtc ccgcggtgag
    69901 gattttaccg cccttctctt ctgcccatgc ggggagcggt gctgccgatg tcaccaccag
    69961 cgctccagcg gcaataccgc tgtgtttaat aaattcacgt cttgttaatg tcataacttc
    70021 ctccctgatc aacgaggatc actgtttctc ggtaatatct ttggcgttgt actggaagta
    70081 ccgcgtcaaa atgtccagtt cgttttcgct catgctggtt cgcgccccca ttcctttggc
    70141 gatagagggc caggcattga cggtgtaatg gtcagcggca ataggcgcat ggcagccagc
    70201 acaataggtg tcatcgagtt tttcagcgta ttgccataac ggtttacgat ccgctaatac
    70261 gggatcggta agcacaccct gtaaagacgc ctgacgccat tgattgccgt actcgtcagc
    70321 ctgccattcc ccgtctaccg tcagcgcctt gataccttct tcacttaatg tggctagcgc
    70381 cagccgttga cctgccgcta ggtagagcgt gttttcactg ccctgcattt gataaccctg
    70441 caacagaacg atcggctgtt tgccactggc atcaacgacg gtgagatcgg taccaggatt
    70501 cacggtagcc agttcgccta tacgagaagg tttgaaagga taaatatgtg cgccattggt
    70561 aactgaagta gcggcctgac tttccagctc atgcgccgcg ttgtcatcca tttttatttc
    70621 tggtggaaaa tgggcaatgc ctttatgaca atcgatacag gtttcgccgt ccttttgcgc
    70681 tttgttatgc attttctgcg cagattcact ttgcgaggca atatccatgg catcaaaaga
    70741 atggcaacta cgacacgttg cagagtcagt tgcttttaat tctttccata ctgtttcggc
    70801 catttcctgg cgatgcgctt cgaacttatc gtcactgtct attttgccgc taacaaattc
    70861 atgataaata tctttagatg cttttaattt agcaaataaa taatctatcc ctgactttgg
    70921 aatatggcaa tcggcacatt ccgcacgtat ccctttctgg ttagaaaagt ggacagttcc
    70981 ctgatattcc tcaaaaggtt tactcatcga gtggcaggaa aggcaaaatg cggtatccga
    71041 cgttttatgt aaggcttttt gcgccagcaa taacccgccg ccaccaacca caaccgctat
    71101 cagcaaaaac attaacccaa tgcgtttttt ccctcgcata gattcctcta attaattcca
    71161 gaacaccgaa atcattgtat aattaaatat acaacgaatt gaccgcttgc ataattcggc
    71221 acaacactgc ctgaaataat cgataaagaa tatgatttat tacaatgtaa tcattaattg
    71281 ctaaagaata acccagttgt tatttagaat atatatttgt cagttttagt aatttaaatt
    71341 acataattaa tgtgaatata taaaaatttt tagcacaacg ctatgcgcac tgtcacgcgc
    71401 tcaactgatc acggaaatcg ttcg
//
LOCUS       DAPOZO010000010        68217 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPOZO010000010 DAPOZO010000000
VERSION     DAPOZO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 68217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.010"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            219..899
                     /gene="rnc"
                     /locus_tag="ROI21_000703"
     CDS             219..899
                     /gene="rnc"
                     /locus_tag="ROI21_000703"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDX5109668.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            896..1801
                     /gene="era"
                     /locus_tag="ROI21_000704"
     CDS             896..1801
                     /gene="era"
                     /locus_tag="ROI21_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDX5109669.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            1813..2541
                     /gene="recO"
                     /locus_tag="ROI21_000705"
     CDS             1813..2541
                     /gene="recO"
                     /locus_tag="ROI21_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDX5109670.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            2553..3284
                     /gene="pdxJ"
                     /locus_tag="ROI21_000706"
     CDS             2553..3284
                     /gene="pdxJ"
                     /locus_tag="ROI21_000706"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDX5109671.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADVEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            3284..3664
                     /locus_tag="ROI21_000707"
     CDS             3284..3664
                     /locus_tag="ROI21_000707"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDX5109672.1"
                     /translation="MTILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            4084..4164
                     /gene="shoB"
                     /locus_tag="ROI21_000708"
     CDS             4084..4164
                     /gene="shoB"
                     /locus_tag="ROI21_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDX5109673.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(4359..4619)
                     /gene="yfhL"
                     /locus_tag="ROI21_000709"
     CDS             complement(4359..4619)
                     /gene="yfhL"
                     /locus_tag="ROI21_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDX5109674.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(4675..5523)
                     /locus_tag="ROI21_000710"
     CDS             complement(4675..5523)
                     /locus_tag="ROI21_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDX5109675.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            5732..6367
                     /gene="yfhb"
                     /locus_tag="ROI21_000711"
     CDS             5732..6367
                     /gene="yfhb"
                     /locus_tag="ROI21_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDX5109676.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRINLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            6392..6928
                     /gene="tadA"
                     /locus_tag="ROI21_000712"
     CDS             6392..6928
                     /gene="tadA"
                     /locus_tag="ROI21_000712"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDX5109677.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITQGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(6925..8481)
                     /gene="mltF"
                     /locus_tag="ROI21_000713"
     CDS             complement(6925..8481)
                     /gene="mltF"
                     /locus_tag="ROI21_000713"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDX5109678.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNSNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            8739..12626
                     /gene="purL"
                     /locus_tag="ROI21_000714"
                     /gene_synonym="purI"
     CDS             8739..12626
                     /gene="purL"
                     /locus_tag="ROI21_000714"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDX5109679.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            13183..14610
                     /gene="qseE"
                     /locus_tag="ROI21_000715"
     CDS             13183..14610
                     /gene="qseE"
                     /locus_tag="ROI21_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDX5109680.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLQATACLAEPMLLMSVLDNLYSNAVHYGTESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            14775..15488
                     /gene="qseG"
                     /locus_tag="ROI21_000716"
     CDS             14775..15488
                     /gene="qseG"
                     /locus_tag="ROI21_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDX5109681.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDSQALQLQLAEER
                     QRYGKLQQSSDSELDTLRQQYHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            15478..16812
                     /gene="glrR"
                     /locus_tag="ROI21_000717"
     CDS             15478..16812
                     /gene="glrR"
                     /locus_tag="ROI21_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDX5109682.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            16873..17211
                     /gene="glnB"
                     /locus_tag="ROI21_000718"
     CDS             16873..17211
                     /gene="glnB"
                     /locus_tag="ROI21_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDX5109683.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(17256..18446)
                     /gene="hmpA"
                     /locus_tag="ROI21_000719"
     CDS             complement(17256..18446)
                     /gene="hmpA"
                     /locus_tag="ROI21_000719"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDX5109684.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGLSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            18775..20028
                     /gene="glyA"
                     /locus_tag="ROI21_000720"
     CDS             18775..20028
                     /gene="glyA"
                     /locus_tag="ROI21_000720"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDX5109685.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(20092..21285)
                     /locus_tag="ROI21_000721"
     CDS             complement(20092..21285)
                     /locus_tag="ROI21_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDX5109686.1"
                     /translation="MRACINNQQIRHHNKCVIQELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGCLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATKIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            21403..24684
                     /locus_tag="ROI21_000722"
     CDS             21403..24684
                     /locus_tag="ROI21_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDX5109687.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPERAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIILSYQEHQPQELPLPDVAKA
                     PLAAQDIASTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDTREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDQTEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAHYESARAELTAINPAWPKAALFTTVMPFI
                     FNRVH"
     gene            24781..25764
                     /locus_tag="ROI21_000723"
     CDS             24781..25764
                     /locus_tag="ROI21_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDX5109688.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFIDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIIST
                     SDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            25787..27298
                     /locus_tag="ROI21_000724"
     CDS             25787..27298
                     /locus_tag="ROI21_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDX5109689.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDMMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            27323..28321
                     /locus_tag="ROI21_000725"
     CDS             27323..28321
                     /locus_tag="ROI21_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5109690.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            28354..29448
                     /locus_tag="ROI21_000726"
     CDS             28354..29448
                     /locus_tag="ROI21_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDX5109691.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            29460..30332
                     /locus_tag="ROI21_000727"
     CDS             29460..30332
                     /locus_tag="ROI21_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDX5109692.1"
                     /translation="MTIYTLSHGPLKLDVSDRGGVIEGFWRDATPLLRPGKKNGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCVSRSEDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRTQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFSQLAPLPHQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(30380..30802)
                     /locus_tag="ROI21_000728"
     CDS             complement(30380..30802)
                     /locus_tag="ROI21_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDX5109693.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVILIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAIIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(30899..32101)
                     /locus_tag="ROI21_000729"
     CDS             complement(30899..32101)
                     /locus_tag="ROI21_000729"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDX5109694.1"
                     /translation="MKEKTIIIVGGGQAAAMAAALLRQQGFSGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTQELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAASVMGRNAPPPVQRYLLPRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLRADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AIIRLDNGALHRCESWENANNQAQIAASAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKRLTD
                     ENIALKSL"
     gene            complement(32111..32923)
                     /gene="hcaB"
                     /locus_tag="ROI21_000730"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(32111..32923)
                     /gene="hcaB"
                     /locus_tag="ROI21_000730"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDX5109695.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVNQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALRQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(32920..33240)
                     /locus_tag="ROI21_000731"
     CDS             complement(32920..33240)
                     /locus_tag="ROI21_000731"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDX5109696.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVDGGDIFIDLP
                     EAQP"
     gene            complement(33240..33758)
                     /locus_tag="ROI21_000732"
     CDS             complement(33240..33758)
                     /locus_tag="ROI21_000732"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDX5109697.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWRFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDGNWRLLERDIVLDQ
                     AVISSHNLSVLF"
     gene            complement(33755..35116)
                     /locus_tag="ROI21_000733"
     CDS             complement(33755..35116)
                     /locus_tag="ROI21_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDX5109698.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            35252..36142
                     /gene="hcaR"
                     /locus_tag="ROI21_000734"
     CDS             35252..36142
                     /gene="hcaR"
                     /locus_tag="ROI21_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDX5109699.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            36302..37441
                     /locus_tag="ROI21_000735"
     CDS             36302..37441
                     /locus_tag="ROI21_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDX5109700.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLIARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESAGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTAMPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37433..38713)
                     /gene="csiE"
                     /locus_tag="ROI21_000736"
     CDS             complement(37433..38713)
                     /gene="csiE"
                     /locus_tag="ROI21_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDX5109701.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKVFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(38904..39758)
                     /locus_tag="ROI21_000737"
     CDS             complement(38904..39758)
                     /locus_tag="ROI21_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX5109702.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAENAIATIIHAHSNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(39903..40706)
                     /gene="suhB"
                     /locus_tag="ROI21_000738"
     CDS             complement(39903..40706)
                     /gene="suhB"
                     /locus_tag="ROI21_000738"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDX5109703.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            40825..41565
                     /gene="trmJ"
                     /locus_tag="ROI21_000739"
     CDS             40825..41565
                     /gene="trmJ"
                     /locus_tag="ROI21_000739"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDX5109704.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            41835..42323
                     /gene="iscR"
                     /locus_tag="ROI21_000740"
     CDS             41835..42323
                     /gene="iscR"
                     /locus_tag="ROI21_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDX5109705.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            42435..43649
                     /gene="iscS"
                     /locus_tag="ROI21_000741"
     CDS             42435..43649
                     /gene="iscS"
                     /locus_tag="ROI21_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDX5109706.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            43677..44063
                     /gene="iscU"
                     /locus_tag="ROI21_000742"
     CDS             43677..44063
                     /gene="iscU"
                     /locus_tag="ROI21_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDX5109707.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            44080..44403
                     /gene="iscA"
                     /locus_tag="ROI21_000743"
     CDS             44080..44403
                     /gene="iscA"
                     /locus_tag="ROI21_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDX5109708.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            44499..45014
                     /gene="hscB"
                     /locus_tag="ROI21_000744"
     CDS             44499..45014
                     /gene="hscB"
                     /locus_tag="ROI21_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDX5109709.1"
                     /translation="MDYFTLFGLPACYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            45031..46881
                     /gene="hscA"
                     /locus_tag="ROI21_000745"
     CDS             45031..46881
                     /gene="hscA"
                     /locus_tag="ROI21_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDX5109710.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            46883..47218
                     /gene="fdx"
                     /locus_tag="ROI21_000746"
     CDS             46883..47218
                     /gene="fdx"
                     /locus_tag="ROI21_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDX5109711.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            47230..47430
                     /gene="iscX"
                     /locus_tag="ROI21_000747"
     CDS             47230..47430
                     /gene="iscX"
                     /locus_tag="ROI21_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDX5109712.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            47489..48772
                     /gene="pepB"
                     /locus_tag="ROI21_000748"
     CDS             47489..48772
                     /gene="pepB"
                     /locus_tag="ROI21_000748"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDX5109713.1"
                     /translation="MTEEMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWTFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKSPVEQWSAGATGLGVRTIANLLTA"
     gene            48891..49667
                     /gene="sseB"
                     /locus_tag="ROI21_000749"
     CDS             48891..49667
                     /gene="sseB"
                     /locus_tag="ROI21_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDX5109714.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(50427..51272)
                     /gene="sseA"
                     /locus_tag="ROI21_000750"
     CDS             complement(50427..51272)
                     /gene="sseA"
                     /locus_tag="ROI21_000750"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDX5109715.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLFEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGAFNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            51479..56437
                     /gene="yfhM"
                     /locus_tag="ROI21_000751"
     CDS             51479..56437
                     /gene="yfhM"
                     /locus_tag="ROI21_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDX5109716.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSEKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQVIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSVSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDVINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRSEEAIALALKTPRNDERKWL
                     GDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFMAART
                     IQDLPGKWQAQTSFSTEPLTGEKAQNSNLNSDQLAALQVTNSGDQPLWLRVDASGYPQ
                     SAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLPA
                     GLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPVT
                     LVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            56438..58750
                     /gene="pbpC"
                     /locus_tag="ROI21_000752"
     CDS             56438..58750
                     /gene="pbpC"
                     /locus_tag="ROI21_000752"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDX5109717.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADAEGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQTPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLGDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRLRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASEHRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            58899..59330
                     /gene="ndk"
                     /locus_tag="ROI21_000753"
     CDS             58899..59330
                     /gene="ndk"
                     /locus_tag="ROI21_000753"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDX5109718.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            59480..60634
                     /gene="trmG/rlmN"
                     /locus_tag="ROI21_000754"
     CDS             59480..60634
                     /gene="trmG/rlmN"
                     /locus_tag="ROI21_000754"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDX5109719.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            60919..61932
                     /gene="rodZ"
                     /locus_tag="ROI21_000755"
     CDS             60919..61932
                     /gene="rodZ"
                     /locus_tag="ROI21_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDX5109720.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            61959..63077
                     /gene="ispG"
                     /locus_tag="ROI21_000756"
                     /gene_synonym="gcpE"
     CDS             61959..63077
                     /gene="ispG"
                     /locus_tag="ROI21_000756"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDX5109721.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFEQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            63188..64462
                     /gene="hisS"
                     /locus_tag="ROI21_000757"
     CDS             63188..64462
                     /gene="hisS"
                     /locus_tag="ROI21_000757"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDX5109722.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            64480..65100
                     /gene="yfgM"
                     /locus_tag="ROI21_000758"
     CDS             64480..65100
                     /gene="yfgM"
                     /locus_tag="ROI21_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDX5109723.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            65111..66289
                     /gene="bamB"
                     /locus_tag="ROI21_000759"
     CDS             65111..66289
                     /gene="bamB"
                     /locus_tag="ROI21_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDX5109724.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNETDGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            66407..67879
                     /gene="der"
                     /locus_tag="ROI21_000760"
     CDS             66407..67879
                     /gene="der"
                     /locus_tag="ROI21_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDX5109725.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     TPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            67942..68157
                     /locus_tag="ROI21_000761"
     CDS             67942..68157
                     /locus_tag="ROI21_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDX5109726.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVIA"
BASE COUNT        16215 a        17048 c        18841 g        16113 t
ORIGIN      
        1 gagataaatc tcccgtggct aacgacatcc cccgtcgttg tgtatagaat attcccccga
       61 agtttaaggt tggcacctcc aggttgccac ggcacacgaa acagcgttgg tccccatata
      121 ccggtaaact gaaactgcag cgaagcagtt agcagaacca tgtatatcag gtctgtttcg
      181 tgtgctgaat tgttgacgca tttatttatt ggtatcgcat gaaccccatc gtaattaatc
      241 ggcttcaacg gaagctgggc tacactttta atcatcagga actgttgcag caggcattaa
      301 ctcatcgtag tgccagcagc aaacataacg agcgtttaga atttttaggc gactctattc
      361 tgagctacgt tatcgccaat gcgctttatc accgtttccc tcgtgtggat gaaggcgata
      421 tgagccggat gcgcgccacg ctggtccgtg gcaatacgct ggctgaactg gcgcgcgaat
      481 ttgagttagg cgagtgctta cgtttagggc caggtgaact taaaagcggt ggatttcgtc
      541 gtgagtcaat tctcgccgac accgtcgaag cattaattgg tggcgtattc ctcgacagtg
      601 atattcaaac cgtcgagaaa ttaatcctta actggtatca aacccgtctg gacgaaatta
      661 gcccaggcga taaacaaaaa gatccgaaaa cgcgcttgca agaatatttg cagggtcgcc
      721 atctgccgct gccgacttat ctggtcgtcc aggtacgtgg cgaagcacac gatcaggaat
      781 ttactatcca ctgccaggtc agcggcctga gtgaaccggt ggttggcaca ggttcaagcc
      841 gtcgtaaggc tgagcaggct gccgccgaac aggcgttgaa aaaactggag ctggaatgag
      901 catcgataaa agttactgcg gatttattgc tatcgtcgga cgtccgaacg ttggcaaatc
      961 cacattattg aacaaactgt tggggcagaa gatctctatc acctcccgca aggcgcagac
     1021 aactcgtcac cgcattgtgg ggatccatac tgaaggcgcg tatcaggcga tctacgtcga
     1081 taccccgggc ctgcatatgg aagaaaaacg cgccattaac cgcctgatga acaaagcggc
     1141 gagcagctcc attggcgacg ttgagctggt gatttttgtc gttgaaggca cccgctggac
     1201 gccggacgac gaaatggtgc tcaacaagct gcgtgatggc aaagcgccgg taatcctcgc
     1261 ggtgaacaaa gtggacaacg tgcaggaaaa agccgatctg ctgccacact tacagttcct
     1321 ggcaagccag atgaacttcc tcgatatcgt gccgatctct gctgaaaccg ggctgaatgt
     1381 cgacactatc gcagcaatcg tgcgtaagca tctgcctgaa gcgacccatc acttcccgga
     1441 agattacatc accgatcgct cacagcgttt tatggcgtct gaaatcatcc gcgaaaaact
     1501 gatgcgtttc ctcggcgctg aactgccgta ctccgtgacc gtggagatcg aacgtttcgt
     1561 ctctaacgaa cgaggcggtt atgacatcaa cggtttgatt ctcgttgagc gtgaagggca
     1621 gaagaagatg gttattggca acaaaggggc caagatcaaa accatcggga ttgaagcgcg
     1681 taaagacatg caggaaatgt tcgaagcgcc tgttcacctt gagctgtggg taaaagtgaa
     1741 atccggttgg gccgacgacg aacgcgcact gcgcagtctc ggttacgttg acgatcttta
     1801 agagtaactc cgatggaagg ctggcagcgc gcatttgtcc tgcatagtcg cccgtggagc
     1861 gaaaccagcc tgatgctgga cgtcttcacg gaggaatcgg gtcgcgtgcg tctggttgcc
     1921 aaaggcgcac gctctaaacg ctctactctg aaaggtgcat tacagccttt cacccctctc
     1981 ttgctacgtt tcggcgggcg tggcgaagtc aaaacgctgc gcagtgctga agccgtctcg
     2041 ctggcgctgc cattaagcgg tatcacgctt tacagcggtc tgtacatcaa cgaacttctc
     2101 tcccgcgtac tggaatacga gacgcgcttc tctgaactct ttttcgatta cttgcactgc
     2161 attcagtctc ttgccggggt cactggtacg ccagaacccg cgctgcgccg ctttgaactg
     2221 gcactgctcg ggcatctggg ctatggcgtc aattttaccc attgtgcggg tagcggcgag
     2281 ccggtagatg acaccatgac gtatcgttat cgcgaagaaa aagggtttat cgcaagcgtc
     2341 gttatcgaca ataaaacgtt caccggaagg cagttaaaag cgttaaacgc gcgggaattt
     2401 cctgacgcag acacactgcg cgccgcgaaa cgctttaccc gcatggcgct taagccgtat
     2461 cttggcggta aaccattaaa gagcagggaa ctgttccggc agtttatgcc taagcgaacg
     2521 gtgaaaacac attatgaatg atgaggattg tcatggctga attactgtta ggcgtcaaca
     2581 ttgaccatat tgcaacgctg cgtaacgcac gcggtactgc ttacccggat ccggtgcagg
     2641 cggcgtttat tgctgagcag gcgggagcag acggcattac cgtgcattta cgtgaagatc
     2701 gccgccacat tactgaccgt gatgtgcgca tcctgcgtca gacgctggat acccgcatga
     2761 atctggagat ggcggtgacc gaagagatgc tggcgatcgc cgttgaaaca aagccgcatt
     2821 tttgctgcct ggtacctgaa aagcgtcagg aagtaacaac cgaaggcggc ctggacgtcg
     2881 cagggcagcg tgaaaaaatg cgcgatgcct gcaaacgtct ggcagatgcc gggatccagg
     2941 tttctctgtt tatcgacgcc gatgtagagc agatcaaagc tgcggcagag gttggcgcac
     3001 cgtttatcga gatccacaca ggttgctatg ctgatgccaa aactgacgcc gaacaggcgc
     3061 aagagctggc gcgtatcgcc aaagccgcga cctttgccgc tagtctcggt ctgaaagtta
     3121 acgctggtca cggtctgacc tatcacaacg tgaaagctat tgccgccatc cctgagatgc
     3181 atgaactgaa tatcggtcat gccattattg gtcgtgcagt gatgaccgga ctgaaagatg
     3241 cggtggcaga aatgaagcgt ctgatgctgg aagcgcgtgg ctaatgacaa tattaggttt
     3301 aggtacggat attgtggaga tcgctcgcat agaagcggtg atcgcccgat ccggtgagcg
     3361 cctggcacgc cgcgtattaa gcgataacga atgggcaatc tggaaaacgc accaccagcc
     3421 ggtgcgtttt ctggcgaagc gttttgctgt gaaagaagcg gcggcaaaag cgtttggcac
     3481 cgggatccgc aatggtctgg cgtttaatca atttgaagta tttaatgatg agctcggcaa
     3541 accacggcta cggctatggg gtgaggcatt aaaactggcg gaaaagctcg gcgttgtaaa
     3601 tatgcatgta acgctggcag atgaacgcca ctatgcttgt gccacggtaa ttattgaaag
     3661 ttaaaaaaat accgttctct ttataaagta aaacccccga actggtaagg aacgggggtt
     3721 ttacttttta actttaattt tgtgcagcac gcaccctcaa caagagcgat tatggatcag
     3781 ggcggttatc ttttacaagc aacttgcaat ctttagcata aaaactcgag cctttacgaa
     3841 gaaaacaata ttgatggaaa gattaacgtg accgccaatt cggaagtaca ttaaaattgg
     3901 cttcgttatt ggcgattttg ctgtgcttta taccatgcca cagaattccc ccattgaaac
     3961 gagtggtgtc gtcaaagctc tggtgtggag tgcagcatgc accctcaata actcgcacgt
     4021 tcagttttgg ggagatgtaa gggctaatct gaacggctgc attccttgtt taaggaaaaa
     4081 cgaatgactg attgccgata ccttattaaa cgggtcatca aaatcatcat tgctgtttta
     4141 cagctgatcc ttctgttctt ataacataag gaaacgtact taaggcgcgt caggttaacc
     4201 agccaggcgc gcctttaata attataaata agtgcttata cagatagtgt tatcattcat
     4261 gaaataattg atatttatga tcacatcacg aaaataaata taagaacctg aaggctcaca
     4321 ctataaagtc aacaatagtc tactccagga gcagaatatt aaattttatc cgcgtgatgc
     4381 atcagcacaa atttatccca caactgttct tccgtctcga catgttgcgg atccttcaca
     4441 atagtattgg ggatcgggca caccttctgg caggttggtg tctcgtagtg ccctacgcat
     4501 tcggtacact tatcgctgtt aatctcatag atatgatcgc ccattgaaat cgcctcattc
     4561 gggcattcgg gttcacacat atcacaattg atgcattttt tagtgattaa cagcgccata
     4621 aggaaactct caaaacaaca caaaccgggc gggcattata cgcgcattct ttactcagac
     4681 cagttttttc accagcgctt cactgtgacg aatacgttct ggagccagtt ccagatcctg
     4741 ctgaatcagc gcaatgaaca gcaaatccgt taacattccc tgcgcgtgac aagcagagat
     4801 tgaagcactg tttgtcgctt gttcttcggc aatggtatac aggcaatgag aagcacgctg
     4861 ttgcagggca ttcggagtaa agccggtaat cgccagcact tttccgccca ctcgcagcat
     4921 ctcatctgcc gccaggttta actcgcgtcg tacaccggtg taagaaatgg ctaataacag
     4981 atcgtcaggg gacgacgcct gtacggtagc gagtagcgca tgcatatcgc gcactgcggc
     5041 agcattgaag ccaatcttca tcagcttcca ggcaaagttt tgcgccacca gacccgaagc
     5101 gccaatgccg gtcagaatta tccgccgcgc agagcgcaac attgttacgc attcatgcag
     5161 tttctcttca ctattaacgt ttagcgttgc gtacatagcg gcagtatttt ctttaatcag
     5221 tttttcgccg accagccgta acggatcatc accgcggatt tggttatgaa tgggcacgga
     5281 gggcgattcc ggctggcttg ccagcgcttc actcaacgcc aacttaagcg ccggaaaacc
     5341 tttatagccg agtttttgcg cgaacttcac gacgctggac tgactgactc cggcttcgtt
     5401 ggccagttgc tgagagctta aatggcgcgc cgtatcaggt tgtagcagaa gataatccgc
     5461 cagtttttta tcactctggg caagcccctg gtaacgctga cggatacgaa gtaagccgtt
     5521 catatctttc ctcgcgatga aatagtggcg acaacaggca atatgagaca aacgcgagtg
     5581 aattttatat tccattgccg ctaatttcca tgaattaaaa attccagtta gccatgatgc
     5641 ggtcgattgt tgccggaaaa gtgatgatct atctgttagg ctatgtagta gcgaaaatta
     5701 atcaggatgt ttcagtccag aggagtatgg tttggcaact cacgagcgtc gtgtggtgtt
     5761 ttttgactta gatggaacat tgcatcagca ggatatgttc ggcagttttt tgcgttattt
     5821 actgcgtcgc caaccgctga atgcgttgct tgtcctgccg ttgttgccga tcatagccat
     5881 tgcgttattg ataaaaggcc gtgcggcacg ctggccgatg agtctgcttc tgtggggatg
     5941 cacttttggt cacaatgaag cgcgtttgca ggcgttgcag gccgatttcg tacgctggtt
     6001 tcgtgacaat gttaccgcct ttccgctggt tcaggagcga ttaaccacct acctgttaag
     6061 ttccgatgct gatatctggt tgattaccgg ctctccgcag ccgctggttg aagcggttta
     6121 tttcgatacg ccctggctgc cgcggattaa tcttatcgcc agtcagattc agcgtggcta
     6181 tggtggttgg gtattgacga tgcgttgtct gggacatgaa aaggtcgcgc aactggagcg
     6241 taaaatcggc acgccactgc ggttgtacag tggctatagc gacagtaatc aggacaaccc
     6301 gctgctttat ttctgtcagc atcgttggcg agtaaccccg cgcggtgaac tccagcaact
     6361 ggaatagagt aaagcatagc gtccgtgtat aatgcgccgc gcttttataa ccggagtttt
     6421 ctttttgtct gaagtcgaat ttagccacga atactggatg cgtcacgcgc tgacgctggc
     6481 aaagcgtgcc tgggatgagc gggaagtgcc ggtcggcgcg gtattagtgc ataacaatcg
     6541 tgttattggc gaaggctgga accgtccgat tggtcgacat gatcccaccg cacatgcaga
     6601 aatcatggct ctgcggcagg gcggtctggt gatgcaaaat tatcgtctga tcgacgccac
     6661 gttgtatgtc acgcttgaac cctgtgtaat gtgtgccgga gcgatgatcc acagtcgcat
     6721 tggtcgcgtg gtctttggtg cgcgtgacgc gaaaactggc gctgcgggat ctttaatgga
     6781 tgtgctgcat catccgggta tgaatcaccg agtggaaatt acccaaggaa tactggcgga
     6841 tgagtgcgcg gcgttgctca gtgacttctt tcgcatgcgc cgccaggaaa ttaaagcgca
     6901 gaaaaaagcg caatcctcga cggattaatt ttgtttctct tcactccctt tcctggaaaa
     6961 cagcagagag ggagaatggg tcaaatagtt tgatgacgac tgggaaagaa acgagagaaa
     7021 aggaaatttc tctttgccca actccgtagg cgataccgcc ggataatcct gcgccagttg
     7081 catcgccgct tctgtagcct gcttctcttt ctcttgcaga taacccacca ggctaatctg
     7141 atacttacga atattttcga cataagcgta ggcttcatgc ccacgagcgt agccgtaagt
     7201 cagcttgctg taatagggtt tctggctaag taaaggcaga cgctgtttta cgtcagccca
     7261 actgtcagga ttgcctttgg tttttgccgt cagagcgcgg gcatccagca tatgcgcata
     7321 gcccatattg tatgcggcga gggcaaacca aatccgttcg ttctccggca cactttccgg
     7381 cactttactc atcatatcct gcaaataacg cacgccgccg ctgatgctct gttcggcatc
     7441 ggtacgatcc gtaatgccga ggctttgcgc ggtatttttg gttagcatca tcatgccgcg
     7501 cacacccgtc ggtgaagtgg cctgcgcatc ccagtgcgat tcctgataag caatagcggc
     7561 cagcaaacgc cagtcaattt cttcggcgta tttctcaaac aggggcttta actgcggcag
     7621 taccgcatcg acggcgcgta aaaatgtgcg cgtatcgacg taatcaaaat catcgccatg
     7681 ccccaggtat ttctcttcaa tgcgtgccag agtaccgtct tcattcattt cgttgaagaa
     7741 atcgagcaga gcggcggaaa gggtattatc gccatctaat gggctaaacc aggtcaccgg
     7801 ttgttcatcg gtaatgtcga gcgctacggc gagttccgga tgaacgcgct gaaacaggct
     7861 aatggcaaca gaatcagcaa tggtgtaatc gagttttcct tcgatgacat cttccattaa
     7921 ttccgcagag ccttttttgt cgtcaacctt ccagcttaat tccgggaatt ttgtttcttt
     7981 cagggtctgg agatcgttaa ccaccacatg acccggtgca acggtgagtt gctccgccgt
     8041 caggttgccc agtgtacgtg ggcgatactg acccacttta taaaccagtt gttgtgaaac
     8101 ggagtagtag gtcgggcctg gctgataatt tttaaccctt tcgctgttat agaccagtcc
     8161 tgccgccagc aggtcggcgt tactattatc gaggtcgtca aacagctggc tgatattctg
     8221 ccgcacggtc actttcagtt ttacgccgag gtaatcggca aactgtttcg ccagttcgta
     8281 atccaggcca aaaggtttcc cgttgatttc gttatacgtc aggggagtat gaatggtgct
     8341 cacacgcaac tctccccgcg cttgaatggc ggcgatacgg ttgtcggctt taccaaacca
     8401 gggaatggat ggccagagag cgaccgcgag cagcagtgcc agaatgccga tgaacagata
     8461 attaatcttt aattttttca attagttaat tctctgtgtc gtgcgcgtcc cagcttgaaa
     8521 aaaagtaata atagtgaaag gtttactcat aaatgagcgg cattttgcgt aaacctgcgc
     8581 cagttggcaa cttattacag ccagtggcgg cacgcgttgc taattcgcga tggtgatttt
     8641 atttccacgc aaacggtttc gtcagcgcat cagattcttt ataatgacgc ccgtttcccc
     8701 cccttgggta caccgaaagc ttagaagacg agagacttat gatggaaatt ctgcgtggtt
     8761 cgcctgcatt gtcggcattc cgaatcaaca aactgctggc acgttttcag gctgccaggc
     8821 tcccggttca cactatttac gccgagtatg tccattttgc tgacctcaat gcgccgttaa
     8881 acgatgatga gcacgcacaa cttgaacgcc tgctgaaata tggcccggca ctcgccagcc
     8941 acgccccgca aggcaaactc ctgctggtga ccccgcgtcc tggcaccatc tctccctggt
     9001 cttcgaaagc gaccgatatt gcccataact gcgggctgca acaggtaaac cgtcttgagc
     9061 gcggcgtggc ttactatata gaagccggta cgctgaccaa tgaacaatgg cagcaggtta
     9121 ccgctgaact gcacgaccgc atgatggaaa cggtcttttt tgctttagat gatgcagagc
     9181 agctgtttgc acaccatcaa ccgactccgg tcaccagcgt tgatttgctg gggcagggcc
     9241 gtcaggcgct aatcgacgct aacctgcgtc ttggcctggc actggcggaa gatgaaattg
     9301 actatttgca ggatgctttc acaaagcttg gtcgtaaccc gaacgacatc gaactgtata
     9361 tgtttgccca ggcgaactcc gagcactgcc gccacaaaat ttttaacgcc gactgggtta
     9421 tcgatggtga acagcagccg aaatcgctgt ttaagatgat caaaaacacc ttcgaaacca
     9481 cgccggatca cgttctctct gcttataaag ataacgccgc cgtaatggaa ggttctgaag
     9541 tgggccgcta ctttgctgac cacgaaacgg gccgctacga tttccatcag gaaccggcgc
     9601 atattctgat gaaagtcgaa actcacaacc acccgacggc gatttctccg tggccgggcg
     9661 cggcgaccgg ttccggcggt gaaatccgcg atgaaggtgc caccgggcgc ggcgcgaagc
     9721 cgaaagctgg tttggttggt ttctccgttt ccaacttgcg tattccgggc tttgaacaac
     9781 cgtgggaaga agatttcggt aagcctgagc gcattgtcac cgcgctggac atcatgaccg
     9841 aaggcccgct tggtggcgcg gcatttaaca acgaatttgg tcgtccggca ctgaacggct
     9901 acttccgtac ttatgaagaa aaagtgaata gccacaatgg cgaagagctg cgcggttatc
     9961 acaaaccgat catgctggcg ggcgggatcg gcaacattcg cgccgatcac gtacaaaaag
    10021 gcgagatcaa cgttggtgcg aagctggtcg ttctcggcgg cccggcaatg aacatcggtc
    10081 ttggcggcgg cgcggcgtct tccatggcgt ctggtcagtc tgatgccgac ctcgactttg
    10141 cttccgtaca gcgcgacaac ccggagatgg agcgtcgctg ccaggaagtg atcgaccgtt
    10201 gctggcagct tggtgatgcc aacccaatcc tgtttatcca cgacgtcggc gctggcggtc
    10261 tttctaacgc catgccggaa ctggtgagcg acggcgggcg cggcggtaaa ttcgaactgc
    10321 gcgatatcct gagcgacgaa ccgggcatga gcccgctgga aatctggtgt aacgaatccc
    10381 aggaacgcta cgtgctggcg gttgctgccg atcagttacc gctgtttgac gaactgtgta
    10441 agcgtgagcg cgcgccatac gcggtgattg gtgaagcgac cgaagaactg catctttctt
    10501 tgcacgatcg tcattttgat aatcagccga tcgatctgcc gctggacgtc ctgcttggta
    10561 aaacgccgaa gatgacccgc gaagtacaaa cgctgaaagc gaagggtgat gcgctggttc
    10621 gtgaagggat caccatcgct gacgcggtga aacgcgtact gcatctgccg actgtggcag
    10681 agaaaacttt cctggtgacc atcggtgacc gcagcgtaac tggcatggtg gcgcgcgatc
    10741 agatggtggg tccgtggcag gtgccggtcg ctaactgcgc ggtcactacc gccagccttg
    10801 acagctacta tggtgaagcg atggcgattg gcgagcgtgc gccggttgcg ctgctagatt
    10861 tcgccgcctc tgcccgtctg gcggtcggtg aagcgttaac caatattgcc gcaacacaga
    10921 ttggcgatat caaacgcatt aaactttccg ccaactggat ggcggcggca ggccaccctg
    10981 gtgaagatgc gggcctgtat gaagccgtta aagctgtggg cgaagaactt tgtccggcgc
    11041 tgggcctgac gatcccggtg ggtaaagact ctatgtcgat gaaaacccgc tggcaggaag
    11101 gtaacgaaga gcgcgaaatg acgtcgccgc tgtcgctggt gatttctgca tttgcccgtg
    11161 tggaagatgt gcgtcacacc atcacgccgc agctttctac cgaagataac gcattactgc
    11221 tgattgatct gggcaaaggc aataacgcgc tgggcgcaac ggcgctggcg caggtttatc
    11281 gtcagcttgg cgacaaaccg gcagatgtac gcgacgttgc ccaactgaaa ggcttctatg
    11341 acgcgattca ggcgctggtt gcacagcgta agctgctcgc gtaccacgac cgctcggatg
    11401 gcggcctgct ggtaacgctg gcggaaatgg cctttgctgg tcattgcggc attgacgcgg
    11461 atatcgccac tctgggtgac gatcgcctgg cggcgttgtt taacgaagaa ctgggtgcgg
    11521 tgattcaggt tcgtgccgct gaccgtgaag cagttgaagc ggtactggca cagcatgggc
    11581 ttgcggattg cgtccattat gttgggcagg cggtttccgg tgaccgtttt gtgattaccg
    11641 ccaacgggca gactgtattc agcgaaagcc gcaccacgtt gcgtgtctgg tgggcagaaa
    11701 ctacttggca gatgcagcgc ctgcgtgaca acccggagtg tgccgaccag gagcaccagg
    11761 cgaaatcaaa cgacgccgat ccgggcctga atgtaaaact gtcgttcgat atcaacgaag
    11821 atgtggcagc accgtatatt gccactggcg cacgtccgaa agttgctgtg ctgcgtgagc
    11881 agggcgtgaa ctcgcatgtt gaaatggcgg cagctttcca ccgtgcaggc ttcgatgcta
    11941 tcgacgtgca tatgagcgac ctgctggctg gacgcacggg cctggaggat ttccacgccc
    12001 tggtcgcgtg cggtggtttc tcctacggtg atgtgctggg tgccggtgaa ggttgggcga
    12061 agtctatcct gttcaatgac cgtgtacgcg atgagtttgc aaccttcttc caccgtccgc
    12121 aaacgctggc gctgggggta tgtaacggtt gccagatgat gtctaatctg cgcgaactga
    12181 tcccaggtag tgagttgtgg ccacgttttg tgcgcaatac ctccgatcgc tttgaagcgc
    12241 gtttcagcct ggttgaagta acccaaagcc cgtctctgct gttgcagggg atggtgggct
    12301 cgcaaatgcc gattgctgta tctcatggtg aagggcgcgt ggaagtgcgt gatgcggcgc
    12361 atctggcggc actggaaagc aaagggctgg tggcactgcg ctatgtcgat aacttcggta
    12421 aagtcactga aacctacccg gctaacccga acggttcacc gaacggtatt acggcggtta
    12481 cgactgaaag tggtcgagtc accattatga tgccgcaccc ggaacgtgtt ttccgtactg
    12541 tcagcaactc ctggcatccg gaaaactggg gcgaggatgg gccgtggatg cgtattttcc
    12601 gcaatgcacg taagcaattg ggataagccg tcaacccatt ggttttgcag ccgcatccgg
    12661 cattaagcac caaccgctat tataagctcc caacttcggg agcttttttg tgtctgtaaa
    12721 tcacgacaat gggtggttta ccgtgtcgct ttctggcgac acttaactga ttgattttaa
    12781 tagtatctaa taagtttatc ttaagttgtc tctttttagc gacacagtgg ctgatttaca
    12841 ttcaaatagc gaagaagcca taaatccctc aaagcagcgt ataatcagta cgttaccaaa
    12901 ctattttctt tattggcaca gttactgcat aatagtaagc agtggctcat tcaccgactt
    12961 atgtcagccc cttcgggacg tgctacataa aattcgaatg acgcacaaca aggtgcctgc
    13021 cgtccaactt ctgatatcag cgtagctata tcaaccatcg ggcgaaacgt cgagttaggc
    13081 accgccttat tccataacaa agccgggtaa ttcccggctt tgttgtatct gaacttcccc
    13141 tcggttagca tcaggctatt cgcgtctgac gagagtaaca ccttgaaacg ctggcccgtt
    13201 tttccccgct cattacgaca actggtaatg ctggcatttt tgctgattct gctgcccctg
    13261 ttggtgctgg catggcaagc ctggcaaagc ctgaatgcgc ttagcgatca ggcggcgctg
    13321 gttaaccgca ctacgcttat tgatgcccgg cgcagtgaag cgatgaccaa cgcggcgctg
    13381 gagatggagc gtagttaccg tcagtattgc gtgctggacg accctacgct tgcgaaggtt
    13441 tatcaaagcc agcgcaagcg ttacagcgaa atgctcgatg cccacgcagg cgtactgcca
    13501 gacgataaac tttaccaggc attgcgtcag gatttgaaca atctggctca acttcagtgt
    13561 aacaacagtg gtcccgatgc cgccgccgcc gcgcgtcttg aagcctttgc cagtgccaat
    13621 accgaaatgg tgcaggccac gcgcacagtg gtgttctctc gtgggcagca acttcagcgt
    13681 gaaatcgccg aacgtgggca atattttggt tggcaatcgc tggtgctatt tctggtgagt
    13741 ctggtgatgg tgctactttt cacgaggatg attatcggtc cggtgaaaaa tatcgagcgc
    13801 atgatcaacc ggctggggga agggcgttct ctgggcaata gcgtttcgtt cagtggaccg
    13861 agcgagttac gctcggttgg gcaacgtatt ctttggttaa gtgagcgcct gtcatggctg
    13921 gaatcccaac gtcatcaatt cttaagacat ttatctcatg aattaaaaac gccgctggcg
    13981 agtatgcgcg agggcactga attactggct gaccaggttg tcgggccgct tacgccagag
    14041 caaaaagagg tggtgagcat cctcgatagc agcagccgca atttgcaaaa actgatcgaa
    14101 caactgcttg attacaaccg taaacaggcg gacagtgcgg tggaactgga gaatgttgaa
    14161 ttagcaccgc tggtggagac agtagtttct gctcatagcc tgcccgcacg ggctaaaatg
    14221 atgcataccg acgtcgatct ccaagcaaca gcttgcctgg cggagccaat gctgctgatg
    14281 agcgtactgg ataatcttta ctccaatgct gtgcactacg ggactgaatc cggtaacatt
    14341 tgccttcgca gcagtttaca tggcgcgcgg gtttatattg atgtcatcaa tacaggaaca
    14401 cccattccgc aagaggaacg cgccatgatc ttcgaaccct tttttcaggg aagccaccag
    14461 cgaaaagggg cggtgaaggg cagcggtctg ggattaagca ttgccaggga ttgtattcgc
    14521 cgtatgcaag gggaactgta tctggtcgac gagagcgggc aagacgtttg cttccgcatt
    14581 gaattgccgt cgtcgaaaaa cacgaaataa tgctaacagc ctatcccatc aggctatttt
    14641 atttgccagt ttggccctgg gcagtgctcg aaatcctcac gtacgacatg tacgctccgg
    14701 tttctccgcg ctgtccgtat ccaaactgcc tgcaacaatt acgcccactg ggaaaggcgc
    14761 taagtctggt gaatatgcga cacatttttc aacgattatt gccccgacgg ctgtggctgg
    14821 cagggctgcc atgtctggca ttgctgggtt gcgtgcagag tcacaataag ccagccattg
    14881 atacgccagc agaagaaaaa attccggttt atcaactggc tgactatctt tctactgaat
    14941 gtagcgacat ctgggcgctg caaggtaaat caaccgaaac caatccgctt tactggctgc
    15001 gggcgatgga ttgtgctgat cgtttaatgc ctgcgcagtc gcgtcagcaa gcccgccaat
    15061 atgacgacgg cagctggcaa aataccttta aacaagggat tttgctcgcc gacgccaaaa
    15121 ttacaccgta tgagcgccgc cagctggtcg cgcgcattga tgcgctaagt accgagatcc
    15181 cggcacaggt tcgccctctg tatcaactct ggcgcgacag tcaggcgctg caactgcaac
    15241 tggcggaaga gcgccaacgc tacggcaagc tccagcaatc cagcgacagc gagctggata
    15301 ctttgcgcca gcaatatcac gttctacagc agcaactgga actcaccacc cgcaagctgg
    15361 aaaacctgac tgatattgaa cgccagctct cgacccgcaa accggctgga aatttctcgc
    15421 cggatacgcc acacgagagt gaaaagcccg cgccatccac cgatgaggtc actcctgatg
    15481 agccataaac ctgcgcattt attattggtc gatgacgatc cgggattgct gaaactgctt
    15541 ggcctgcgcc tgaccagcga aggctacagc gtggtcacgg cggaaagtgg tgctgaagga
    15601 ttacgggtac tgaatcgcga aaaagtagat ttagttatca gcgacctgcg gatggatgaa
    15661 atggatggta tgcagctgtt tgctgaaatc cagaaagtgc agccgggaat gccggtaatt
    15721 attcttaccg cacatggttc tattcccgat gccgttgctg caacacagca gggagtgttc
    15781 agtttcctca ccaagcctgt cgataaagac gcgctatatc aggcaattga tgatgcgctg
    15841 gaacaatccg cgccagccac cgatgaacgc tggcgcgagg cgattgtcac ccgcagcccg
    15901 ctgatgctgc gtttgctgga acaggcgcgg ctggtggcgc aatcagacgt cagcgttttg
    15961 attaatggcc agagcggcac cgggaaagag attttcgccc aggctatcca caacgctagc
    16021 ccgcgcaata gcaaaccatt tattgctatt aactgtggtg cattgcccga gcaattgctg
    16081 gagtcggagc tgtttggtca tgcgcgtggc gcgtttactg gcgctgtcag caatcgcgaa
    16141 ggtttattcc aggcggcgga aggcggtacg ctatttctcg atgagattgg cgatatgccc
    16201 gcaccgttgc aggttaaact gctgcgcgtg ttgcaggagc gtaaagtgcg cccgctgggc
    16261 agtaaccgcg atattgatat cgacgtgcgg attatttccg ccactcaccg cgacctgcca
    16321 aaagcgatgg cgcgcgggga attccgtgaa gatctctatt accgcctcaa cgttgtcagc
    16381 ctgaaaattc cggctctggc agagcgcaca gaagacattc cgctactggc aaatcacctg
    16441 ttgcgccagg cggcagagcg acataaaccg tttgtccgcg cgttctccac cgatgcgatg
    16501 aaacgcctga tgaccgctag ctggccgggt aatgtacgcc agttggtcaa cgtgattgaa
    16561 cagtgcgtgg cgctgacctc atctccggtg attagcgatg cattagtaga gcaggcgctg
    16621 gagggcgaaa acacggcgct gccaaccttt gttgaggcgc gtaatcagtt tgaactcaac
    16681 tacttgcgta agctgctgca aattaccaaa ggcaacgtca cccacgcggc gagaatggcg
    16741 gggcgcaacc ggacagaatt ttataaactg ctatcccgac acgagctgga tgcaaacgat
    16801 ttcaaggaat gaattggcgt tatgtgttac gtttagcaga tcaaaagaca ggcgaccttt
    16861 tcaaggaata gcatgaaaaa gattgatgcg attattaaac ccttcaagct ggacgatgtt
    16921 cgcgaagcac tggctgaagt cggcattact ggcatgacgg tcactgaggt gaaaggtttt
    16981 ggccgccaga aaggccatac cgagctgtac cgcggcgcgg agtatatggt ggattttctg
    17041 ccgaaagtga aaattgagat tgtcgtaccg gacgacattg tcgatacctg tgtcgatacc
    17101 attattcgca cggcgcaaac cggcaaaatc ggtgatggta aaatcttcgt ctttgacgtg
    17161 gcacgggtca ttcgtatccg taccggtgaa gaagacgacg cggcaattta aaccgcgtca
    17221 agggttgccg gatgtttcca tccggcaaca tcaaatcaca gcaccttatg cgggccaaag
    17281 cattcgtaat gaatgttttc ctgcttcacg cccagatcca ctaactgttt cgcggcaaac
    17341 tgcatgaagc caaccgggcc gcagagatag aactgcattg tcggatcgct gaacgcacct
    17401 tccagtttgc tcaaatccat cagaccttcg ctatcaaact gacctttagc gcgatcggct
    17461 tcgctcggct gacgatacca ggtgtgcgcg gtaaagcgcg gcagtgacag ccccagttcc
    17521 ttaacttcat cagcaaaggc gtgaacatcg ccattttctg ccgcatggaa ccagttcact
    17581 tgtgctgtgt ggccagcttt tgccagcgtg tcgagcattg ccagcattgg cgtttgaccg
    17641 acaccggcag agattaacgt cactggtgtg tcatctgcga cagccataaa aaaatcacct
    17701 gctggagcga ccagtttcac gacatcgcca acattggcgt gattgtgcag ccagttggat
    17761 acctgcccac cctcttcgcg tttcaccgca atacgatagc ctttgccatc cggtttgcga
    17821 gtcaaagagt actgacgaat ttcctgatgc gggaaacctt ccggcttcag ccagacgccg
    17881 agatattgcc ccggacggta ttctgccact gcgccaccgt cgaccggctc cagttcgaag
    17941 ctggtgataa gcgcgctgcg cggtgtttta gccacaatgc ggaaatcgcg agtaccttcc
    18001 cagccaccgg ctttgctggc gttttcgtta tagatttccg cctcgcgatt gataaataca
    18061 ttagccagta cgccataggc tttaccccac gcgtccagca cttcctggcc cgggctgaac
    18121 atttcgtcca gcgtcgccaa caggtgttca ccgacaatgt tgtactgttc cggtttgatc
    18181 tggaagctgg tgtgcttctg cgcgattttt tctaccgctg gcagcagcgc aggcaggttt
    18241 tcaatattac tggcgtaggc ggcaatagcg ttaaacaggg cttcacgttg atcgccattg
    18301 cgctggttac tcatgttaaa aatttctttg agttctgggt tatgagtaaa catacggtcg
    18361 tagaaatggg cggttaactt tggtcccgtt tccaccagta aagggatggt ggcttttact
    18421 gtagcgatgg tttgagcgtc aagcatatgg tcttcctttt ttttgcatct taattgatgt
    18481 atctcaaatg catcttataa aaaatagccc tgcaatgtaa atggttcttt ggtgtttttc
    18541 agaaagaatg tgatgaagtg aaaaatttgc atcacaaacc tgaaaagaaa tccgtttccg
    18601 gttgcaagct ctttattctc caaagccttg cgtagcctga aggtaatcgt ttgcgtaaat
    18661 tcctttgtca agacctgtta tcacacaatg attcggttat actgttcgcc gttgtccaac
    18721 aggaccgcct ataaaggcca aaaattttat tgttagctga gtcaggagat gcggatgtta
    18781 aagcgtgaaa tgaacattgc cgattatgat gccgaactgt ggcaggctat ggagcaggaa
    18841 aaagtacgtc aggaagagca catcgaactg atcgcctccg aaaactacac cagcccgcgc
    18901 gtaatgcagg cgcagggttc tcagctgacc aacaaatatg ctgaaggtta tccgggcaaa
    18961 cgctactacg gcggttgcga gtatgttgat atcgttgaac aactggcgat cgatcgtgcg
    19021 aaagaactgt tcggtgctga ctacgctaac gtccagccgc actccggctc ccaggctaac
    19081 tttgcggtgt acaccgcgct gctggaacca ggtgataccg ttctgggtat gaacctggcg
    19141 catggcggtc acctgactca cggttctccg gttaacttct ccggtaaact gtacaacatc
    19201 gttccttacg gtatcgatgc taccggtcat atcgactacg ccgatctgga aaaacaagcc
    19261 aaagaacaca aaccgaaaat gattatcggt ggcttctctg catattccgg cgtggtggac
    19321 tgggcgaaaa tgcgtgaaat cgctgacagc atcggtgctt acctgttcgt tgatatggcg
    19381 cacgttgcgg gcctggttgc agctggcgtc tacccgaacc cggttcctca tgctcacgtt
    19441 gttactacca ccactcacaa aaccctggcg ggtccgcgcg gcggcctgat cctggcgaaa
    19501 ggtggtagcg aagagctgta caaaaaactg aactctgccg ttttccctgg tggtcagggc
    19561 ggtccgttga tgcacgtaat cgccggtaaa gcggttgctc tgaaagaagc gatggagcct
    19621 gagttcaaaa cttaccagca gcaggtcgct aaaaacgcta aagcgatggt agaagtgttc
    19681 ctggaacgcg gctacaaagt ggtttccggc ggcactgata accacctgtt cctggttgat
    19741 ctggttgata aaaacctgac cggtaaagaa gcggacgccg ctctgggccg tgctaacatc
    19801 accgtcaaca aaaacagcgt accgaacgat ccgaagagcc cgtttgtgac ctccggtatt
    19861 cgcgtgggta ctccggcgat tacccgtcgt ggcttcaaag aagccgaagc gaaagaactg
    19921 gctggctgga tgtgtgacgt gctggacagc atcaatgatg aagccgttat cgagcgcatc
    19981 aaaggtaaag ttctcgacat ctgtgcacgt tacccggttt acgcgtaagc gaaacggtta
    20041 atctgttgaa aaaaggcttc cttcgggaag cctttctacg ttatcgcgcc atcaaatctg
    20101 tcgtaactgc gcctcaacat acaaatagcc aatccccagc acctgttgtg cgcggcttaa
    20161 ttgcccaaag ccaattttcg tcgctttcac gctcggtcct tcgtcgcggt cgaacgggtt
    20221 aaatcccgtc tggcgaataa tggtattaag ccagtgctca ccaaaggcac aactgcgacc
    20281 gtacaaccaa atctgattaa tattgaggat gttgaggaag ttatacagac tcaaaccaat
    20341 ggcattggca gagcgatcaa cccagctggt gatccacggt tctccactct gccaggcagc
    20401 gattaactgc gctgttgtca gtttttcagg atcaagctga gtattaaccg gttgtgattt
    20461 tagccatacc cgcgcctgtt tttttaatgc gcttaacgag gcgacggttt ccaggcagcc
    20521 ataacgtccg cagtcgcaga cgacgccatc cggattaacg atggtgtgac caatctgtcc
    20581 gctaccatac aaacagccgc gataaatttg ctcgttaatc acgaacgacg agccaatgcc
    20641 gtaatcaacg ttgatcacgc agaaatcccg ttcctgcgaa ttattttgcc atttctccgc
    20701 cagcgccagc atcacgcagt cattatcgac catcacccga atgccgagct tctcttccag
    20761 cagatacttt acctcaaccg gcgttgtcca gggcgcttgc ggcatggttt gcgacactcc
    20821 ggtcacagga tcaacctgac cgtggattgc cagcgccaga ttgatggtgc ggtccggcca
    20881 caatttacgg tggcgatgcc agcatttttc gatttcggac agcagcgcct gcggcgtcgg
    20941 tgcatcaatc tgtaaatact caaattcacc tttcggactt aaacaggcgt tagcgacctg
    21001 acactcaata ctggtgggcg ttacgttcag acacagcgtc cagtcacctt ccggcgcaat
    21061 cagccatgta ccgctactat gcccgcgcgt ctggctttca tcgtcgatat tcaccacccg
    21121 tttttcgctt tccagttcct gcaaaatatt actgactgcc ggaatcgaaa tttgcgccag
    21181 ccgggccagc gttgatttat tggcgcgctt ttgccggtac agcagttcct gaatcacgca
    21241 tttgttatgg tggcgaatct gttgattatt aatgcaggct ctcatcttca ctaaaccttg
    21301 tttagttaat tgcgtgatta ttccgcgaaa acattgccag attgttcaat acactgccac
    21361 aaatctttta attcagtatg tcttgttaat attgagggca ccatgactcc agtaaaagtg
    21421 tggcaagagc gcgttgagat cccgacctat gaaaccgggc cgcaggatat acatcctatg
    21481 ttcctggaaa atcgcgttta tcagggatcg tccggcgcgg tttatcccta cggcgtgacc
    21541 gatacgctga gcgagcagaa aaccctgaaa tcctggcagg cggtgtggct ggaaaacgac
    21601 tacatcaaag tgatgatcct gccggagctg ggcggtcggg tgcatcgcgc gtgggataaa
    21661 gtgaaacaac gcgattttgt ctatcataat gaagtcatta aaccggcgct ggtggggctg
    21721 ctggggccgt ggatctccgg cgggattgag tttaactggc cgcaacacca tcgcccgacc
    21781 acctttatgc ccgtcgattt caccctcgaa gcccatgaag acggcgcaca aaccgtgtgg
    21841 gtcggcgaaa cggagccgat gcacggttta caggtgatga caggtttcac cctgcgccct
    21901 gaacgggcgg cgctggaaat cgccagccgc gtctataacg gcaacgccac gccgcgtcat
    21961 ttcttgtggt gggccaaccc ggcagtgaaa gggggtgaag ggcatcagag cgtcttcccg
    22021 ccggatgtaa cggcggtgtt tgatcacggc aaacgggccg tctccgcttt ccccatcgcc
    22081 accggcactt actacaaagt ggactactcc gccggagtgg acatttctcg ctataaaaat
    22141 gtgcccgttc caacctcata tatggctgaa aaatcacagt acgattttgt tggcgcgtgg
    22201 tgtcacgatg aagatggcgg tttgctgcac gttgccaacc accatattgc gccaggtaaa
    22261 aaacagtgga gctggggaca cagtgaattt ggccaggcgt gggataagag tctgaccgac
    22321 aataacggcc cgtatatcga actgatgact ggtatttttg ccgataacca gcccgatttt
    22381 acctggcttg atgcttacga agagaagcgt tttgagcagt atttcctgcc ttaccattct
    22441 ttgggcatgg tgcaaaacgc ctcccgcgat gcggtgatta aactccagcg tagcgagcgg
    22501 gggattgagt gggggctgta tgccatctct ccgttgaaag gataccgtct ggcgatccgc
    22561 gaaatcggca aatgcaacgc gttgctcgat gatgccgtgg ccctgacacc agcgaccgcc
    22621 atccagggcg tgttgcacgg tatcaatcct gaaaggctga ccattgaact ctccgatgcc
    22681 gacggcaata tcatactgag ttatcaggaa catcagccgc aagagttgcc gttgccggac
    22741 gtcgccaaag cgccactggc agcacaagac attgccagta cagatgaagc ctggtttatc
    22801 ggtcagcatc tggagcaata tcatcacgcc agccgttcac cgttcgatta ctacctgcgc
    22861 ggcgtggcgc tggatccgct ggattatcgc tgtaacctgg cgctggcgat gctggaatat
    22921 aaccgcgccg atttcccgca agcggtggcg tatgccactc aggcgctgaa acgcgcacat
    22981 gcgctgaaca aaaatccgca gtgcggacag gccagtttga ttcgcgccag tgcttacgaa
    23041 cgtcagggac aatatcaaca agccgaagag gatttctggc gtgcggtctg gagcggcaat
    23101 agtaaagcag gcggctatta tggtctggca cgactggcgg cgcgtaatgg tcacttcgat
    23161 gctggtctgg atttttgcca acaaagtctt cgcgcctgcc caaccaatca ggaagtgctt
    23221 tgcctgcata atctgctgct ggtgttaagt ggtcgtcagg acaacgcgcg tttgcagcgc
    23281 gagaaactgc tgcgcgatta tccgctgaac gccactctgt ggtggctgaa ctggttcgat
    23341 ggtcgtagcg aatcagccct cgcgcagtgg cgcggtctgt gtcagggacg cgacgttaac
    23401 gccctgatga ccgccgggca actgattaac tggggaatgc ccaccctggc ggcagagatg
    23461 ctaaacgcac tggactgcca gcgcacgctg ccgctttacc tgcaagccag cttgctgccg
    23521 aaagctgaac gcggcgaact ggtcgcaaaa gccattgatg tcttcccaca gtttgtccgt
    23581 ttcccgaata cgctggaaga agtggcggcg ctggagagta ttgaagagtg ctggtttgcc
    23641 cgtcatttac tggcctgctt ctactacaac aaacgtagct acaacaaagc cattgcctta
    23701 tggcaacgtt gcgtagagat gtcgccggag tttgccgacg gctggcgcgg cttagcgatc
    23761 catgcgtgga ataagcaaca cgattatgag ctggccgcgc gttatcttga taatgcttat
    23821 cagcttgcgc cgcaggatgc acgtctgctt ttcgaacggg atttgcttga taagttaagt
    23881 ggagccacac cggagaaacg actggcgcgt ctggaaaata atctggaaat tgcgctgaaa
    23941 cgcgacgaca tgaccgcaga actgctcaat ttgtggcatc tcacgggtca ggcagacaaa
    24001 gcggcggaca ttctcgccac gcgcaaattc cacccgtggg aaggcgggga agggaagatc
    24061 accagtcagt ttatcctcaa ccagttatta cgcgcctggc agcatcttga taccagagag
    24121 ccgcagcagg ccagcgaact gcttcatgcc gcgctgcatt atccggagaa tttaagcgaa
    24181 ggccgtttac cggggcaaac tgataacgac atctggttct ggcaggcgat atgcgccaac
    24241 gcgcagggcg atcaaactga agcgatgcgt tgtttacgtc tggcggcgac tggcgatcgc
    24301 accattaaca ttcacagtta ttacaacgat cagccggttg attatctctt ctggcaagga
    24361 atggcgctgc gactgctggg tgaacagcaa accgcacagc aactgtttag tgaaatgaaa
    24421 cagtgggcgc aagagatggc gaaaaccagt attgaggcgg atttctttgc tgtttcacaa
    24481 cctgacctgt tgtcgctgta tggcgattta caacagcagc ataaagaaaa atgcctgatg
    24541 gtggcgatgc tggcgtccgc gggactcggg gaggttgcgc actatgaatc tgctcgcgct
    24601 gaattgacgg cgattaatcc ggcctggccg aaagcagcat tattcaccac cgtgatgcct
    24661 tttattttta accgcgttca ctaaacctta ttaactgtaa tatttgagcg gcacgcgctg
    24721 ccgctcacgc tcaaataatt aaaataagcc gttgtaaata aaaaccctac aggaggcact
    24781 atgcctaaaa aaatgagaac aacccgtaat ctattattaa tggctacgct attaggtagc
    24841 gcactatttg ccagggctgc ggataaagaa atgaccattg gcgcaatata ccttgatacc
    24901 cagggatatt acgctggcgt gcgtcagggc gttcaggatg cggcaaaaga ttcttcagta
    24961 caggtacagt taattgaaac taacgcccag ggtgatattt cgaaagaaag tacctttatt
    25021 gataccctcg tggcgcgtaa tgtcgatgcc attattttat cggcagtgtc tgaaaatggc
    25081 agtagtcgta ccgttcgtcg cgccagtgaa gcgggtattc cggtgatttg ctacaacacc
    25141 tgtattaatc aaaagggtgt cgataaatat gtctcggcat atctggttgg tgacccgctg
    25201 gaatttggta aaaaactggg taacgctgcc gccgattatt ttattgccaa caaaattgac
    25261 cagccgaaaa ttgccgtcat caattgcgaa gcctttgaag tttgtgtgca acgacgtaaa
    25321 gggtttgaag aagtattaaa agcccgcgtt cccggcgcgc aaattgtcgc taatcaggaa
    25381 gggactgttt tagataaagc gatttccgtt ggtgaaaaac taattatctc cacgtcggat
    25441 ctcaacgcca ttatggggga atcgggcggt gcgacactcg gcgcggtaaa agcggtacgt
    25501 aatcaaaatc aggccggaaa aattgctgtt ttcggttcgg atatgaccac tgaaattgct
    25561 caggagctgg aaaacaatca ggtgctgaaa gcggtggtgg atatttccgg taagaaaatg
    25621 ggcaatgctg ttttcgcgca aactttgaag gttatcaata aacaagccga cggtgaaaaa
    25681 gtgattcagg tgccaatcga tctctatacc aaaacggaag acggtaaaca gtggctggca
    25741 acgcacgttg atggtctgcc ctaatcgctg attcgcagag gtccctatgt tcacggcaac
    25801 agaggcagtc ccggtagcaa aagtggtggc aggaaataag cgttatcccg gcgtcgttgc
    25861 gttggataac gttaacttca cgctcaataa aggcgaagtt cgtgcgctgt taggaaaaaa
    25921 cggcgcgggc aaatcgaccc tcattcgaat gcttaccggc agtgaacgtc cggatagcgg
    25981 tgatatctgg attggcgaga cgcgactgga aggtgacgaa gctacgttga ctcgccgtgc
    26041 cgctgaactg ggggttcgtg cggtttatca ggagttaagt ctggtggagg gactgacggt
    26101 ggcggaaaac ctctgcctcg gtcagtggcc ccgccgcaac ggcatgattg attacctgca
    26161 aatggcgcag gatgcccaac gttgcttaca ggcgctgggc gttgacgtta gccctgaaca
    26221 acttgtttca acgctaagcc cggcacaaaa gcaactggtg gaaattgcgc gggtgatgaa
    26281 gggcgagccg cgcgtggtca ttcttgatga acctaccagt tcgcttgcca gtgcggaagt
    26341 tgaactggtg atcagtgcgg ttaaaaagat gtcggcactg ggcgtggcgg tgatttatgt
    26401 cagccaccgg atggaagaaa tccgccgcat tgcctcctgt gccaccgtta tgcgcgatgg
    26461 tcaggtggcg ggcgatatga tgctcgaaaa cacctccacg catcatattg tgtcgctaat
    26521 gctcgggcgc gatcacgttg atattgcgcc ggttgcccct caggaaattg tcgatcaggc
    26581 tgtgctggaa gtccgtgcgt tacgccataa gcccaagctg gaggatatca gctttacgct
    26641 acgtcgtggc gaagtactcg gcattgctgg cctgctgggg gcagggcgca gtgaattgct
    26701 gaaggcgatt gttgggctgg agacgtatga acagggcgaa attgttatca acggcgagaa
    26761 aatcacgcgc cccgattacg gcgacatgct gaaacgcggc attggctata cgccagaaaa
    26821 ccgcaaagaa gcggggatca ttccctggct gggcgttgac gaaaatacag tgctgaccaa
    26881 tcggcaaaaa atcagcgcca acggtgtgct gcaatggtcc accatccgcc gcctgaccga
    26941 agaggtgatg cagcggatga cggtcaaggc cgccagtagc gaaacgccca tcggcacgct
    27001 ttctggcggc aatcagcaaa aagtggtgat cggtcgttgg gtctatgccg ccagccagat
    27061 tttgttgctc gacgagccaa cgcgtggcgt cgatatcgaa gccaaacagc agatttaccg
    27121 tattgtccgc gagctggctg ccgaaggaaa aagtgtggtg tttatctcca gtgaagtgga
    27181 ggagttgccg ctggtgtgtg accgcatcct gttattacag cacggtacgt tctcgcagga
    27241 gtttcactcg ccggtcaatg tggatgagct gatgtccgcc attctgtctg tgcactgatg
    27301 aattttaccg aggaacctga agatgtctgc ttcgtcatta ccattgccgc agggcaagag
    27361 cgtctcgctc aaacaatttg tcagtcgcca tattaatgag atcggtttgc tggtggtgat
    27421 cgccattctt tatctggtct tctctctgaa cgcgccgggc tttatctcat tgaataacca
    27481 gatgaacgtg ctgcgcgatg ccgccaccat cgggatcgcc gcctgggcga tgacgctgat
    27541 tattatctcc ggtgaaattg atgtcagcgt tgggccgatg gtggcttttg tctcggtgtg
    27601 cctggcattt ttgctgcaat ttgaagttcc gttggcgatt gcttgtctgc tggtgttgct
    27661 gttaggcgcg ctgatgggaa cgctcgccgg ggtgctgcgc ggcgtgttta acgtgccaag
    27721 tttcgtggcc acgctggggc tgtggagcgc cctgcgcgga atggggctgt ttatgacgaa
    27781 cgccttgcca gtgccgattg acgaaaacga agtgctggac tggctggggg gacaatttct
    27841 cggtgtgccg gtatccgcgc tgatcatgat ggtgttattt gcgctgtttg tgttcattag
    27901 ccgcaaaacc gccttcgggc gctcggtttt tgctgttggc ggtaatgcca ctgcagcaca
    27961 gttgtgcggt attaacgttc gtcgggtacg cattcttatt tttacccttt cgggattatt
    28021 agcagcggtg accggcattt tgttggcggc gcgcctcggt tccggtaacg caggtgccgc
    28081 aaacggtctg gagtttgacg tcatcgccgc ggtcgtggtt ggcggtacgg cgctttccgg
    28141 tggtcgcggt tccctgtttg gtacattgct tggtgtgttg gtgattacgc taatcggtaa
    28201 cggtctggtg ctgctcggga ttaactcctt tttccagcag gtggtgcgcg gcgtcatcat
    28261 cgtggtggcg gtgctggcga atatcttgct gacccagcgt agcagtaaag cgaaacgcta
    28321 aaccttaacg cccctggcgc gggtgcggca gggatgaaaa ttaatcgtat tgttaatgag
    28381 ggatttatga aaacgatgct ggcagcttat ttaccaggaa attcgaccgt cgatctgcgg
    28441 gaagttgcgg tgccgacgcc ggggattaac caggtactga tcaaaatgaa atcctccggg
    28501 atttgcggaa gtgatgtcca ctatatctat catcaacacc gtgccacggc agcggcaccc
    28561 gataaaccgt tataccaggg atttatcaac ggtcatgaac cgtgcgggca gattgtggcg
    28621 atggggcaag gctgccgcca ttttaaagag ggcgaccgcg tgctggtgta tcacatttct
    28681 ggctgtggtt tttgcccgaa ctgccgccgc ggctttccta tctcttgtac tggcaaagga
    28741 aaagcggctt acggctggca gcgtgacggc ggccatgctg aatacctgct ggcagaagaa
    28801 aaagatctga tcctgctccc ggacgcgctg agctatgaag atggtgcgtt tatcagttgc
    28861 ggcgttggta cagcgtatga agggattttg cgcggcgaag tttccggcag tgataacgtg
    28921 ctggtggtcg gtctggggcc ggtcggcatg atggcgatga tgctggcgaa aggtcgcggt
    28981 gcaaaaagga tcatcggcgt tgatatgctg ccggaacgtc tggcgatggc gaaacagtta
    29041 ggggtgatgg atcacggcta tttagcaacc accgaaggtc tgccgcagat tatcgccgaa
    29101 ctcacccacg gtggcgcgga tgttgcgctc gattgttccg gtaatgccgc aggtcgctta
    29161 ctggcgttgc aatccactgc tgactgggga cgggtggttt acatcgggga aaccggaaaa
    29221 gtggagttcg aggttagcgc tgatctgatg catcatcaac ggcggattat tggctcctgg
    29281 gtgaccagtc tattccatat ggaaaaatgc gcccacgatt taacggactg gaaactgtgg
    29341 ccgcgtaatg ccattaccca tcgcttctca ctggaacagg caggagatgc ctatgcgctg
    29401 atggcgagcg gtaaatgcgg gaaagttgtg attaacttcc cggattaagg agtcagcgga
    29461 tgaccatcta taccttatct cacgggccgc taaagctgga cgtttctgac cggggcggcg
    29521 tcatcgaagg cttctggcgt gacgcgacgc cgctcttacg tcccggtaaa aaaaacggtg
    29581 tggcaaccga cgcctcttgt tttccgctgg tgccgtttgc aaaccgggtg agcggcaatc
    29641 gttttgtctg gcaggggcgt gagtatcaac tgcaaccgaa tgttgagtgg gatgcgcact
    29701 atctgcacgg cgacggctgg ctggggcagt ggcaatgtgt ctcgcgcagc gaagatagct
    29761 tgtgtctggt gtatgaacat cgcagcggtg tttatcacta tcgggtaagt caggcgtttc
    29821 atttaacggc agatacgctg acggtgacgc tctctgtcac caatcaaggg gcagagacgc
    29881 tgccatttgg taccggctgg catccttatt ttccgttgtc accacaaacg cggactcagg
    29941 cgcaggcgag cggttactgg ctggagcggg agcagtggct ggcgggtgag ttttgcgagc
    30001 agctaccgca agagctggat ttcagccagc tcgcgccgtt gccgcaccag tgggtgaaca
    30061 atggctttgc cggatggaat ggtcaggccc gcatcgagca gccgcaggag ggttatgcca
    30121 tcatcatgga aacgacgcca cctgcaccgt gttatttcat ctttgtttcc gacccggcgt
    30181 ttgataaagg atatgcgttt gatttcttct gtctggaacc gatgagccat gcgccggatg
    30241 accatcatcg cccggaaggg ggtgatctca ttgcgcttgc accaggggaa tcaacaactt
    30301 cagagatgtc gttgcgggtt gcgttactgt aatttataaa atgccggata aggcgttcac
    30361 gccgcatccg gcatctttcc taacgccgat cgagagaaat tgcccccggc ccggtaattg
    30421 ccaacagcag gaatgcgcca gcgatactga cattcttcca gaaattaatc atatttggcc
    30481 caaccgcatc gccggtcata tcccagtaat ggtgaccaat tatcgctgta cccagcgtgt
    30541 agaaaataaa cagcaccgcc agcggacggg tgaaaaagcc aagcacgatt aatatcgcgg
    30601 cgggcacttc cataactacc gcaataatcg ctgccagcat tggcattggc gcgcccagcg
    30661 aggccatata ttggaccgta ccgtcaaagc ccatcatttt gggaaaacca aaaataatga
    30721 aaattaagat gacggcgata cgggcaatta ataacaaaac ggggcgggca gcaccaaaat
    30781 caaaataacg tagtgagttc ataaccaatc cctttgcaag ataatgtgat ttaaacgtaa
    30841 tacacactct tgcacctcgc cacgccccag gcgttgagat attcgctaat tatcctggtt
    30901 acagtgattt aagcgcgatg ttctcatctg tcagccgttt cgcatcaaac gttttaccgc
    30961 tctggatcca tttgcggatt gggcgaatct cacgtccctg attcagcgtt accgcaccga
    31021 taagcacgcc gttttgcaga ttaaaccaaa tcgccttctg agtttccggg ttgccacgac
    31081 aaagccagtc atcgccacgc atatcgccaa taaactgtaa gttatcactg tactgatcgc
    31141 tccagaacca cggcggcggc agtcgcggaa gcggtagccc caacattgcg gaagcggcaa
    31201 tttgcgcctg gttattggcg ttttcccaac tttcgcagcg gtgtagtgcg ccattatcaa
    31261 gacggatgat tgccacatcg ccaccggcaa agatcgcggg atcgcaggtg cggcaagctt
    31321 catcaatgac aataccattg gcagtatcaa ggttggcctc gcgagccagt tggtcgttgg
    31381 cgctgatacc aataccgtaa atcaccacat cggcccgaag cgtttcgcca ctttgcagcg
    31441 tcagttctac tttttcacca tcgaccacat gttcaatggc attattgagc agaatgcgca
    31501 caccagcctg ctgatggcgc ggtaaaagat agcgttgcac gggcggtggt gcattacggc
    31561 ccatgacgct tgccgccagt tcaatcactg tcaccttaca tccacgctgc gtggcgctgg
    31621 cagccagttc cagaccaata gttccggcac cgacaatcac gactgaccgt tcgggctgca
    31681 gaacttctcg cagtctggcg gcatcaccgg catggcgcag ggtaaagcag cgttctccca
    31741 gtgcatcaag caacggcagc ggtcgagctg ccgcgccggt tgctataaaa agctgatccc
    31801 agtgccagct ttcgccgtta gctaacacta actcttgtgt gtcgcggccc aatgttttga
    31861 tggttacacc ggaatgcaga tgaacattgt tttcctgcca ccagtgagcg ggtaacacag
    31921 actgcaactg tggggaatct tccagcaaca tggatttcga gagcgggggg cgttcataag
    31981 gaagatgttg ctcatcggaa aacagatgca gctcaccgct gaacccttgc tggcgtagca
    32041 aggccgcagc cattgccgcc gcttgcccgc caccgacaat aatgatcgtt ttttctttca
    32101 tcgtgcttcc ttaaagatcc agcccagccg ctacgtggcg aatgccgcga atcgctaaac
    32161 ccgcatcagc gttgatcatc acaccgctta atgcgcgatt attgcgccgc gatgccaaca
    32221 tcacatacgg acccgtaaaa tccgccggtt gcgggaaaaa ttgcagcggt aaaatggcgg
    32281 caattttctc cggcgtcaga gactgcatta tcgaggtttc actttgcctg agcgcctgtg
    32341 ggccgcgcag gtcgctggcc ataccacacg ggccgacgcc attcacccgc actttcggtg
    32401 ccagttcata agccagttgg cgaataagtc cggttgcggc atgtttactg gcggtgtaca
    32461 gcgggcctcc gccgccggga taccaggcgg catttgacag tgtgaaaatc atgctgcctt
    32521 cactggcgat taacgccgga gcgcaggctt ttgcgcccag caggtaaccg agtacgttga
    32581 cgttaaacag ctcgtggaag ccagtttcga gcgtctctgc gggagtatta accagtgagg
    32641 cattgtgatc ccagatgccc gcattgccga taaaacaatc cagcttgcca gaacgagtca
    32701 ggatctgatt aaccgcgcgt tgataatcgg cataacaggt cacgttacct tccaccgcca
    32761 gaatatgttc gccaaatcgc tgacgcagac tggcgacttt agccgccgac agttccagcg
    32821 tggcaacctg cgcgccttct tcgataaatc gctcgaccag cgccagccct aatcccgatc
    32881 cgccgccggt aataaagatg gactcgttat gcagatcgct catggctgcg cctccggtaa
    32941 gtcgatgaag atgtcaccac catcaacgtg tactggataa gtggtgagcg gatcggtggc
    33001 gggcaggcat aacgctttcc ccgttttcag gcaaaaactg gcggcgtgca gcgggcactc
    33061 caccgtggcg tcatcttcca gatacccttc tgacatcgac gcattaccat ggctgcaacg
    33121 atcgttaatg gcataaaact cgccgcctac gttgaacagg gcgatgacgg gcgaggtatc
    33181 gatccggaga gcctcaccct ccggcacatc cgcaacggga cacgcataaa ttcgattcat
    33241 cagaacagta cactcaggtt atgggaggag atgaccgctt gatccaggac gatatcccgt
    33301 tccagcaagc gccagttccc gtcctccaga cggcgaactt tgtcgaaacg cgttccaaca
    33361 tagaatgttt catcgcgctc tttttgtgcc cgataaagca gataattcac cctcacggca
    33421 aatacgtttg ggatgtcggt ttcgcttacc tggcagttgc tgattaagtg acgagtgcgt
    33481 gacggcggtt cttctgccca ggccatgccg gtttccagac gggcgattcg ccgttccagc
    33541 tggtctttgg tgtcattaaa aatccaggtt gtcggcggtt gaacgccttt gcggcggtcg
    33601 cgcgtttgtg cgttaaccgt ggtgcgcatg gtgtaacgaa tctcttcgtc gagctgtgcc
    33661 agccagtctc gaaatctcca gtcatccagc agactggctt cgtgaaagag aaactggcta
    33721 atgcggtgat gtaactccag tgaaacttgc gcactcattt catcacctcc tgctggtaag
    33781 cggcggtttt atcgagcact tcctgccagc tttcgctact caggagatcg gcccagcgtt
    33841 ggtacattcc acgagcggcc gtttctgaga agatatagtt agtaatgcct ggaatgccgt
    33901 cgtcgcgacg cttttcctga ccaagcccca tttccagaca cagtttgctg ttgcgggcgc
    33961 ggtgaccttt cagcaatttc tggatttcac accagttctc cgagtcatcc tgctcgagaa
    34021 aaccagcagg accaaaagca cgagtggcgc tgttttcaaa agcggcttta acttcatcgg
    34081 aggcggcttt gtcagtaata cagaacgccc acacttcaac ttgatcaggg ccgcgcggat
    34141 gccagacgcg gagtgtggca gtgccgttta gccatgaaag cgtggggaat atattgttat
    34201 gacccgccag acgcagggcg cgaacttcac ctaaacgttg ttctgcttcg gcataggttt
    34261 cgcggtaata gctggaaacc gcgccatcga cccagacatt agcatccggt ttttcagtaa
    34321 agaagaaacc gctgccgtga ccgtcctgac caaactgcag cgcatctttg gcggtttccc
    34381 acaccgggcg ggcagtttgt ccatcaccga ggcgcttatc gctgccatca tctttcgccc
    34441 ctaatacctg aacggcagaa gcatggctga acagagcatg atactggtca ctggcgaact
    34501 gctctgccgg gaatttccag ttgcagttga tcacccactt ttgtacgccg ccgacaattt
    34561 cggtgccgcc ttcgcgacga tccagcatgc catccagata ccaggcaatg tcaccaaggt
    34621 aatcacgcag gcccggtgcg ctggtatccc agttgccaaa aatcagccct ttataactct
    34681 ccacacaagg aacttcgttt agtccccagt gggatttaca caacccttgt gggtaggcgc
    34741 gaggttccag cggtacatcg atcaactcgc cgttaatgcc ataagaccag ccgtgatacg
    34801 gacaggtaaa ggcgcgagtg ttgccgcaat ctgcataact cacacgcatg gcccggtggc
    34861 ggcattggtt gagaaacgcc ttaatgctgc cgtctttctg gcgcaccacg acaaccgcat
    34921 cttctcccat gtaggtgtta aagaaatcac cgggttttgg gatctggctt tcgtgggcga
    34981 gaaatagcca gcaacgaccg aaaatgcgct caagctccag ttggtaaatg tccgggtcgg
    35041 tataaatacg cggagtgacc cgaccgtttt gggtgtcaat cagttggtaa atgttcaaat
    35101 ctgagggtgt ggtcatattt tttaatcctg aaagataaga tgtattgacc ttctattttt
    35161 gtgccataag gatgttgctg gttggttgct aatatgtgaa atagttaatt tctaccagct
    35221 gagacgtttt ttcgataaca aggggaaggt gatggaacta cggcatttac gctatttcgt
    35281 cgcagtggcg caggcactga actttacccg tgcggcggaa aaactgcata cctcacagcc
    35341 ttcgttaagc agccagatcc gcgatcttga aaactgtgtc ggtgttccgt tactggtgag
    35401 ggataagcgc aaagtcgcgt tgacggcggc gggagaatgt tttctccagg acgcgctggc
    35461 aatcctcgaa caagcggaaa atgccaaatt acgggcgcgg aaaattgttc aggaagacag
    35521 acaattaacc attggctttg tgccatcggc ggaagtgaat ttactgccaa aagtattacc
    35581 gatgtttcgt ctcagacagc cagatacctt aattgagctg gtgagtttaa tcaccacgga
    35641 acaggaagaa aaaattcgcc gtggtgaact cgacgtcggc ttaatgcgcc atcctgttta
    35701 tagcccggag attgattatc tggagctttt tgacgaacca ttagtggtgg tgttaccggt
    35761 tgatcaccct ttagcacatg aaaaagagat cactgccgcg caactggatg gcgttaattt
    35821 cgtcagtact gatccagcgt attccggttc gcttgcgccg atcgttaaag cgtggtttgc
    35881 gcaagaaaac agccagccaa atatcgtcca ggtggcaacg aatattctgg tgaccatgaa
    35941 tctggtgggg atggggctgg gcgtcacttt gatacccggt tatatgaata attttaatac
    36001 cggccaggtg gtatttcgac ccattgccgg aaatgtgcct tccattgctt tactgatggc
    36061 gtggaagaaa ggggagatga agccggcatt acgcgatttc atcgccattg tgcaggaacg
    36121 tttggcaagc gtaacggcat aattacgcat tatttttgca acatgcgtcg cggatcgcag
    36181 ttttttagtg cttgtgaact ggtgttacag acatcgggaa gcaggctcga atattcttat
    36241 ttttcaactt gcaccaacat tcgattaacc ccagactaac gcctcctgac gggagggact
    36301 catggttttg caatccacgc gctggttggc gctcggctat ttcacatact tttttagtta
    36361 cggcattttt ctacctttct ggagcgtctg gcttaagggg attggtttaa cgccagaaac
    36421 catcggcctg ttattggggg caggtctgat tgcccgtttt ctcgggagtt tgctcatcgc
    36481 gccccgcgtc agcgatcctt ctcgcctgat ttccgccttg cgcgtgctgg cactgctgac
    36541 acttctcttt gctgtcgcat tctgggcggg ggcgcacgta gcgtggctga tgctggtgat
    36601 gattggcttt aacctctttt tctcaccgct ggtaccgttg accgatgcac tggcgaatac
    36661 gtggcaaaag cagttcccgc ttgattacgg caaagtgcga ctgtggggct cggtggcgtt
    36721 tgtcattggc tcggcgctga cgggcaaact ggtcactatg tttgattatc gggtgatcct
    36781 cgcgctgttg acgttgggcg tggcatccat gctgctcggc tttctcatcc gtccgacgat
    36841 tcagccacaa ggggcaagcc gccagcagga gagcgccggt tggtcagcgt ggttggcgct
    36901 ggttcgccag aactggcgct ttctggcctg cgtttgttta ttgcaggggg cgcatgcggc
    36961 ctattacggt tttagcgcca tttactggca ggcagctggc tactcggcct cggcggtggg
    37021 ctatttgtgg tcgctgggcg tggtggcgga agtcattatc tttgcgctga gtaataaact
    37081 tttccgccgt tgtagtgcac gcgatatgct gttgatctcg gcgatttgcg gcgtagtgcg
    37141 ctggggcatt atgggagcaa cgacggcgat gccgtggttg atagtggtgc aaattctgca
    37201 ttgcggcacc ttcacggtct gccacctggc cgccatgcgc tatattgccg ctcgccaggg
    37261 tagcgaagtc atccgtttac aggcggttta ctctgccgtc gcgatgggcg gcagtatcgc
    37321 gatcatgacc gttttcgccg gtttcctgta tcaatatctg gggcacggcg tgttctgggt
    37381 gatggcgctg gtggcgcttc cggcaatgtt tttgcgcccg aaagttgttc cctcatgctg
    37441 attcgagcat tttgcggatt tgctcctgtt gatgtgttgt cagcgtcagg tccgtataga
    37501 tcagcggtgg tgaaaagagc ggtaacggca tggtataagg agcaataatc agtgccgcgc
    37561 cgcgcggagc gcctgtctgc aaaaatacct ttaccgacat atgtttaatg ttgagcggca
    37621 gtaacgttag ttcgcgtagc tgctgctcaa tctgcgcttc tcgctcgcta tcattcccgg
    37681 tcagtagaat aatctgtttc tcatgcaggt cgttttcctg cattagccag gcaccaaaaa
    37741 tcaccgcgac cagaccgctt tcctcatcag aaagatggac gccgtattca ctttcaaatc
    37801 cggccagcgc cgcgcgggtg gtgcgcacca ggcgtgggta caggcgggcg aactcttccg
    37861 gcagggtgtt atcaataccg atggcaaaca aactgcgatt taacgcctgg gcgaggtggg
    37921 tataaagctg atcgcataac ccctgttcat cataaaaacg aacatttccc agctcacgaa
    37981 aatggtttac cagacgtttg atagactgac gcagttgtct gtcccgctga tgcgcatccc
    38041 gcagtggatc gggaacccgc aacatggaaa aaagtaatgc cataaacagt ggctcatcag
    38101 gcggtacagg ctggagcgcc cgacgctgcc agtggcgtcc aatttcctgc gctacctgaa
    38161 attcaaggca ggattccgcc cagcgacgtt ggagcggatt aaactgaggc gtaattccag
    38221 cgtggtgttg cagcaaacaa tattgcagat acagacacag gaaatgaata tcgcgcgttt
    38281 caaagcgttt ttgcagccgt cgggagcaga gattcactaa cgcttgtaga ttggtatcgt
    38341 cgtaaaagtt acgcgcaatt ccgcgccgct tcagttcact cttcagggcg ggggtgaaat
    38401 ggctggtaat aaatgacgga cagagacgga aaccacgtcg tagccagtga aataaacaca
    38461 aacgttggtt aagcactgta ccttcaaccc ggtagctacc gtcataacca gtagtcagtg
    38521 tgagttgatg atagcgcagg atctcccgtc ctgtttcgct gatatcaaga ctggcaatat
    38581 catcatccac accattaagc tcgctgaaag ttgccgtggt ggcggtcaac cccggctgaa
    38641 agagtgtcag caagatctgg cagcggcgct ggggagccga aaggacagat ggtggagcaa
    38701 gcgtaggcat catctgtaga tctcccataa gcataattga taagaatagc taaagtttgc
    38761 tcagggaaat ggcgaaggga atgctaatca tcagaaatgt tggcaagcgt cacagaattt
    38821 ttctcatctt aaactgttct gaccagtgga ttagcatgtc ttaaggtggt tttgtgaatg
    38881 attatcgtgc tagcgatcat gggttagttc tgcggattca acgcgctaag gataaaatcc
    38941 accatctgtt cgcgataaac atcgccgtga cgatcggaaa aagcatcaat gtgttcgcca
    39001 tccgggatta ggatcaaccg ttttggctct tttgccaggc tatacaactt ttcgctgtgc
    39061 tgccatggga taacgtgatc agctttaccg tgaatcagta aaagcgggat cgggctgacg
    39121 ctggcgatgt aattttcgcc gctgtaactc tcatcaagta agtagccact gccggggatc
    39181 atctggttgg cgatggtcgc ataagaggca aatgtggagt cgaggatcac cgcacgtatg
    39241 ccttcacgat caccctgacc aataacatcc agaatattcg ccccgccaat actctgcccg
    39301 aacagcacca gccgttgtgg gtttacatca ctgcgatggc gcaccacatt gatggcactt
    39361 tgcgtatcgt ccagcaatcc ggcctgggac ggcgtgcctt ttgatttacc aaacccgcga
    39421 taatcaaaca taaaaacgtt gaaattacgc tcgggtaacc aactgaccag cggccagtgg
    39481 gcggacatat ttccggcatt gctgtgagca tgaataatgg tggcaatggc attttcagcg
    39541 gggccagttg cagaagggat aaaccaacca tgtaggcgag tgccatcttt agcggtgaat
    39601 tctactgatt cggctgacca gggatcgggg ccgtagattt tgtcgtcagg gtaatagaaa
    39661 aaattgatgg cgatgcgcgg tattaaataa atgcagaagg caacaacaaa gagaataaac
    39721 agaattttga gaacgcgttt gttcactggc agcgccataa cctttccttg cgacttcttt
    39781 atcatccatt aatcggggaa agatggtggg agagtggcga aagaaagtaa agcggtgagg
    39841 aagtatttcg gaatagcgcc tgaaaggcga gggcgggtga gtgacatcac ccgccagagt
    39901 cattaacgct tcagggcgtc gcttaactcg tcacgcatgt tcgccagcat ggctttaaca
    39961 acgcgcgggt taccagcaac gatgttaccg gtcagcatgt agttatgacc accggtgaag
    40021 tcgctgacga tgccgcccgc ttcacgaacc agcagctcgc ctgccgcgaa atcccacgga
    40081 cgcagaccga tttcaaagaa accgtcaacg cgacccgcag caacgtaagc cagatccagc
    40141 gccgcagaac cggtacgacg gaagtctgca cattcgttga acagtttgcc gacgatgttg
    40201 atgtaggtgg tggcgtactg ttttgctttg aacgggaagc cggtcgccag aatagtaccg
    40261 tcgagatcgc gagcggtgct gccgcgcaga cggtagccgt tcagctgtgc gccctgaccg
    40321 cgagtggcgg tgaacagttc gttacgcata ggatcgtaaa ccacagcaac ttcagtgcgg
    40381 cctttgatac gaacggcgat agataccgcg aagtgcggca gacgtttgat aaagttggta
    40441 gtgccatcca gtggatcgat aacccattga acatcctgat cagtaccttc aagttcaccg
    40501 ctttcttcgg tgatgatggt gtgctgtggg taagatttac gaatcgtgtc gataatcacc
    40561 gcttcggcag ctttatctac gttggtcacg aagtcgttac tgcctttctg gctcgcttct
    40621 acagcgtccg gggtttcata gtttttggca attaaattac ccgccttgcg cgctgcgcgc
    40681 acggcgatgt tcagcatcgg atgcatcggt ctctctcact ggatgttaaa gaacgggaaa
    40741 acggcgcaga gtatagcagc gagatcggca tctgtcttcg ttttatgata agatgcgcga
    40801 ataatcttta gacgaagaaa gacaatgctg caaaatattc gaattgtgct ggtggagacg
    40861 tcacacaccg gcaatatggg ttctgttgcc cgtgccatga aaacaatggg attaaccaat
    40921 ctgtggttgg ttaatccact ggtgaaacct gactcccagg cgattgccct ggcagcaggg
    40981 gccagcgatg tgattggtaa tgctcatatc gtcgatacgc tcgacgaagc gttagccggt
    41041 tgtagcctgg tggtgggcac cagcgcacgc tcccgcacgc tgccgtggcc gatgctcgac
    41101 ccgcgcgaat gcggtctgaa aagcgtcgct gaagcggcaa ataccccggt ggcgctggtt
    41161 tttggtcgcg aacgcgtcgg cttgaccaat gaagagttac agaaatgcca ttatcatgtc
    41221 gcgattgccg ctaacccgga atacagttcg ctgaacctgg caatggcggt tcaggttatt
    41281 gcctatgaag tgcgcatggc ctggcttgca actcaggaaa atggtgagca ggtcgagcat
    41341 gaagagacgc cgtatccgct ggtcgatgat ctggagcgtt tttatggtca tctggaacaa
    41401 acgctgctgg caaccggttt tatccgtgaa aaccatccgg ggcaggtgat gaataaattg
    41461 cgtcgtttgt ttacccgtgc gcgcccggaa agccaggaat tgaatatcct gcgcgggatt
    41521 ctggcttcta tagagcagca gaataaaggt aataaggccg aataatagtg gttgcctgat
    41581 gcgacgctta acgcgtctta tcaggcctac aatgtacaga accgtaggtc ggataaggtg
    41641 ttcacaccgc atccgacaac aggtacaaac gccacgataa aaaaatggca ctgaaggtta
    41701 aatacccgac taaatcagtc aagtaaatag ttgaccaatt tactcgggaa tgtcagactt
    41761 gaccctgcta tgcaataccc ccacttttac aataaaaaac cccgggcagg ggcgagtttg
    41821 aggtgaagta agacatgaga ctgacatcta aagggcgcta tgccgtgacc gcaatgcttg
    41881 acgttgcgct caactctgaa gcgggcccgg taccgttggc tgatatttcc gaacgtcagg
    41941 gaatttccct ttcttatctg gaacaactgt tttcccgtct gcgtaaaaat ggtctggttt
    42001 ccagcgtacg tggaccaggc ggtggttatc tgttaggcaa agatgccagc agtatcgccg
    42061 ttggcgaagt gattagcgcc gttgacgaat ctgtagatgc cacccgttgt cagggtaaag
    42121 gcggctgcca gggcggcgat aaatgcctga cccacgcgct gtggcgtgat ttgagcgacc
    42181 gtctcaccgg ttttctcaac aacattactt taggcgaact ggttaataac caggaagtgc
    42241 tggatgtgtc tggtcgtcag catactcacg acgcgccacg cacccgcaca caagacgcga
    42301 tcgacgttaa gttacgcgct taataaaaag tattcagaat caggccggag tgtttaacac
    42361 tccgtaaaca cggtcgtaca tccagccggt agcctggttc cttgcattga gtgatgtacg
    42421 gagtttatag agcaatgaaa ttaccgattt atctcgacta ctccgcaacc acgccggtgg
    42481 acccgcgtgt tgccgagaaa atgatgcagt ttatgacgat ggacggaacc tttggtaacc
    42541 cggcctcccg ttctcaccgt ttcggctggc aggctgaaga agcggtagat atcgcccgta
    42601 atcagattgc cgatctggtc ggcgctgacc cgcgtgaaat cgtctttacc tctggtgcaa
    42661 ccgaatctga caacctggcg atcaaaggtg cagccaactt ttatcagaaa aaaggcaagc
    42721 acatcatcac cagcaaaacc gaacacaaag cggtactgga tacctgccgt cagctggagc
    42781 gcgaaggttt cgaagtcacc tacctggcac cgcagcgtaa tggcattatc gacctgaaag
    42841 aactggaagc agcgatgcgt gacgacacca tcctcgtttc cataatgcac gtaaataacg
    42901 aaatcggcgt ggtgcaggat atcgcggcta tcggcgaaat gtgccgtgct cgaggcatta
    42961 tctatcacgt tgatgcaacc cagagcgtgg gcaaactgcc tatcgacctg agccagttga
    43021 aagttgacct gatgtcattc tccggtcaca aaatctatgg cccgaaaggt atcggtgcgc
    43081 tgtatgtgcg tcgtaaaccg cgcgtacgca tcgaagcgca aatgcatggc ggcggtcacg
    43141 agcgcggtat gcgttccggc actctgcctg ttcaccagat cgttggtatg ggcgaagcct
    43201 atcgcatcgc aaaagaagag atggcgaccg agatggaacg tctgcgcggc ctgcgtaacc
    43261 gtctgtggaa cggcatcaaa gatatcgaag aagtttacct gaacggtgac ctggaacacg
    43321 gtgcgccgaa cattctcaac gtcagcttca actacgttga aggtgagtcg ctgattatgg
    43381 cgctgaaaga cctcgcggtt tcttcaggtt ccgcctgtac gtcagcaagc ctcgaaccgt
    43441 cctacgtgct gcgcgcgctg gggctgaacg acgagctggc acatagctct atccgtttct
    43501 ctttaggtcg ttttactact gaagaagaga tcgactacac catcgagtta gttcgtaaat
    43561 ccatcggtcg tctgcgtgac ctttctccgc tgtgggaaat gtacaagcag ggcgtggatc
    43621 tgaacagcat cgaatgggca catcattaat cggtatcgga atcaggagaa tttataatgg
    43681 cttacagcga aaaagttatc gaccattacg agaatccgcg taacgtgggt tcctttgaca
    43741 acaacgacga gaacgtcggc agcggcatgg tgggtgcacc ggcctgtggc gacgtaatga
    43801 agttgcagat taaagtcaac gatgaaggta tcattgaaga cgcgcgtttt aaaacttacg
    43861 gctgcggttc tgctatcgct tccagctccc tggtcaccga atgggtgaaa gggaagtctc
    43921 tcgacgaagc gcaggcgatc aaaaacaccg atattgctga agaacttgaa ctgccgccgg
    43981 tgaaaattca ctgttctatt ctggcagaag acgcgatcaa agccgccatt gcggactata
    44041 aaagcaaacg tgaagcaaaa taagagttga ggtttggtta tgtcgattac actgagcgac
    44101 agtgcagcag cgcgagtaaa tacctttctg gctaaccgcg gcaaagggtt tggcctgcgt
    44161 ctgggcgtga gaacctccgg atgttcaggt atggcttatg tactggaatt tgttgacgaa
    44221 ccgacgccgg aagacatcgt gtttgaagac aaaggcgtga aagtcgtggt cgatggcaaa
    44281 agcctgcaat ttctggacgg tacgcagctg gacttcgtaa aagaaggcct gaacgaaggg
    44341 tttaaattca ccaacccgaa cgtcaaagat gagtgtggtt gcggcgaaag cttccacgtt
    44401 tgatgcgcat accgataacc ccaccgtggt cgcctgcgcg tggggtttgt tttacctgat
    44461 tcgccgttat cgcggcggat cgcagccctg agaatgttat ggattacttc accctctttg
    44521 gcttgcctgc ctgttatcaa ctcgataccc aggcgctgag cctgcgtttt caggatctac
    44581 aacgtcagta tcatcctgat aaattcgcca gcggaagcca ggcggaacaa ctcgccgccg
    44641 tacagcaatc tgcaaccatt aaccaggcct ggcaaacgct gcgtcatccg ttaatgcgcg
    44701 cggaatattt gctttctttg cacggttttg atctcgccag cgagcagcat actgtgcgcg
    44761 acaccgcgtt cctgatggaa cagctggagc tgcgcgaaga actggacgag atcgaacagg
    44821 cgaaagatga agcgcggctg gaaagcttta tcaaacgtgt gaaaaagatg tttgataccc
    44881 gccatcagtt gatggttgaa cagttagaca acgagacgtg ggacgcggcg gcggataccg
    44941 tgcgtaagct gcgttttctc gataaactgc gaagcagtgc cgaacaactc gaagaaaaac
    45001 tgctcgattt ttaatttctg gaagctaaac atggccttat tacaaattag tgaacctggt
    45061 ttgagtgctg cgccgcatca gcgtcgtctg gcggccggta tcgacctggg cacaaccaac
    45121 tcgctggtgg cgacagtgcg cagcggtcag gccgaaacgt tagccgacca tgaaggccgt
    45181 cacctgctgc cgtctgttgt tcactatcaa cagcaagggc attcggtggg ttatgacgcg
    45241 cgtactaacg cagcgcttga caccgccaac accattagtt ctgttaaacg cctgatgggg
    45301 cgctcgctgg ctgatatcca gcaacgctat ccgcatctgc cttatcaatt ccaggccagc
    45361 gaaaacggcc tgccgatgat tgaaacggcg gcggggctgc tgaacccggt gcgcgtttct
    45421 gcggacatcc tcaaagcact ggcggcgcgg gcaactgaag ccctggcagg cgagctggat
    45481 ggtgtcgtta tcaccgttcc ggcgtacttt gacgatgccc agcgtcaggg caccaaagac
    45541 gcggcgcgtc tggcgggcct tcacgtcctg cgcttactta acgaaccgac cgctgcggct
    45601 atcgcctacg ggctggattc cggtcaggaa ggcgtgatcg ccgtttatga cctcggcggc
    45661 gggacgtttg atatttccat tctgcgctta agtcgcggcg tgtttgaagt gctggcgacc
    45721 ggcggtgatt ccgcgctcgg cggcgatgat ttcgaccatc tgctggcgga ttatattcgc
    45781 gagcaggcgg gcattcctga tcgtagcgat aaccgcgttc agcgtgaact gctggatgcc
    45841 gccattgcag ccaaaatcgc gctgagcgat gcggactccg tgaccgttaa cgttgcgggc
    45901 tggcagggcg aaatcagccg tgaacaattc aatgaactga tcgcgccact ggtaaaacga
    45961 accttactgg cttgtcgtcg cgcgctgaaa gacgcgggtg tagaagctga tgaagtgctg
    46021 gaagtggtga tggttggcgg ttctacccgc gtgccgctgg tgcgtgaacg ggtgggcgaa
    46081 tttttcggtc gtccaccgct gacttctatc gacccggata aagtcgtcgc tattggcgcg
    46141 gcgattcagg cggatattct ggtgggtaac aagccggaca gcgaaatgct gttgctggat
    46201 gtgatcccgc tgtcgctggg cctcgagacc atgggcggcc tggtggagaa agtgatcccg
    46261 cgtaatacca cgattccggt ggcccgcgcc caggatttca ccacctttaa agatggtcag
    46321 acggcaatgt ctatccatgt gatgcagggt gaacgtgaac tggtgcagga ctgccgctcg
    46381 ctggcgcgtt ttgcactgcg tggtatcccg gcgctaccgg ctggcggcgc gcatattcgc
    46441 gtaacgttcc aggtcgatgc cgacggtctt ttgagcgtga cggcgatgga gaaatccacc
    46501 ggcgttgagg cgtctattca ggtcaaaccg tcttacggtc tgaccgatag cgaaatcgct
    46561 tcgatgatca aagactcaat gagctatgcc gagcaggacg taaaagcccg tatgttggca
    46621 gaacaaaaag tagaagcggc gcgtgtgctg gaaagtctgc acggcgcgct ggctgctgat
    46681 gccgcgctgt taagcaccgc agagcgtcag gtcattgacg atgctgccgc tcacctgagt
    46741 gaagtggcgc agggcgatga tgttgacgct atcgaacaag cgattaaaaa cgtagacaaa
    46801 caaacccagg atttcgccgc tcgccgcatg gaccagtcgg ttcgtcgtgc gctgaaaggc
    46861 cattccgtgg acgaggttta atatgccaaa gattgttatt ttgcctcatc aggatctctg
    46921 ccctgatggc gctgttctgg aagcgaatag tggtgaaacc attctcgacg ttgcgctgcg
    46981 taacggtatc gagattgaac acgcctgtga aaaatcctgt gcttgcacca cctgccactg
    47041 catcgttcgc gaaggttttg actcgctgcc ggaaagctca gagcaggaag acgacatgct
    47101 ggacaaagcc tggggactgg agccggaaag ccgtttaagc tgccaggcgc gcgttaccga
    47161 cgaagattta gtggtcgaaa tcccgcgtta cactatcaac catgcgcgtg agcattaaca
    47221 gaggttagta tgggacttaa gtggaccgat agccgcgaaa ttggcgaagc actgtacgat
    47281 gcgtatcccg atcttgatcc gaaaacggtt cgattcaccg atatgcatca gtggatttgc
    47341 gatctggaag atttcgacga cgacccgcag gcatccaacg agaaaatcct cgaagcgatt
    47401 ttgttagtct ggctggacga agccgaataa atgaacgggc tgcctggggg tagcccgttt
    47461 tgctgttaaa cgaataagga taactaaaat gacagaagag atgaaaatta ccctctctac
    47521 ccaacctgcc gacgcgcgct ggggagaaaa agcgacttac agcattaata atgatggcat
    47581 taccctgcat ttgaacgggg cagacgatct ggggctgatc cagcgtgcgg cgcgcaagat
    47641 tgacggtctg ggcatcaaac atgttcagtt aagcggtgaa ggctgggatg cggatcgctg
    47701 ctggacattc tggcaaggtt acaaagcccc gaaaggcacg cgtaaagtgg agtggccgga
    47761 tctggacgat gcccagcggc aggaactgga taaccgcctg atgatcatcg actgggtgcg
    47821 tgacaccatc aacgcaccgg cagaagaatt gggaccatcg caactggcac agcgtgctgt
    47881 tgatctgatc agcaacgtcg cgggcgatcg tgtgacttat cggatcacca aaggtgaaga
    47941 tctgcgtgat caaggttata tggggctgca cacggtcggg cgcggttcag aacgttcccc
    48001 ggtattgctg gcgctggatt acaacccaac aggcgataaa gaagcgccag tgtacgcgtg
    48061 cctggtaggt aaaggtatca cttttgactc cggcggctac agcatcaaac agactgcgtt
    48121 tatggactcg atgaagtcgg acatgggcgg cgcggcaacg gttaccgggg cgctggcatt
    48181 tgccattacg cgcggactga acaagcgcgt gaagctgttc ctctgctgtg cggataacct
    48241 gatcagcggc aacgcgttca aactgggcga tattattacc tatcgcaacg gtaaaaaagt
    48301 tgaagtgatg aacactgatg ccgaagggcg tttggtgctg gccgatggcc tgattgatgc
    48361 cagtgcgcag aaaccggaac tgatcattga tgcggcgacc ctcaccgggg cggcgaaaac
    48421 tgcgctgggt aatgattatc acgcgctgtt cagttttgac gatgcgcttg ccggtcgctt
    48481 gctggcgagt gccgcgcagg agaatgaacc gttctggcgt ctgccgctgg cggagttcca
    48541 ccgcagccag ctgccgtcta actttgcgga actgaacaat accggaagcg cggcgtatcc
    48601 ggcaggcgcg agcacggcgg cgggcttcct gtcgcacttt gttgagaact atcagcaagg
    48661 ctggctgcat atcgactgct cggcgactta ccgtaaatcg ccggttgaac agtggtctgc
    48721 gggcgcgacc gggctaggtg tgcgcacgat tgctaatctg ttaacggcgt aaacgtctta
    48781 tttggcctac aaaattgcga aaatccaata gattgagatt cccttgtagg cctgataagc
    48841 gtagcgcatc aggcacttgt gccccgaacg ttgaatgaat tgtggttctt atgtccgaaa
    48901 caaaaaacga acttgaagat ctgctggaaa aagcagcaac cgaaccggcg caccgcccgg
    48961 cctttttccg tacgctactg gaatccaccg tctgggtgcc tggtacggcg gcgcagggcg
    49021 aggctgtggt tgaagatagc gcgcttgatt tacagcactg ggaaaaagaa gacggcacca
    49081 gcgtcattcc ttttttcacc tcgttagaag cacttcaaca ggcggttgaa gacgaacagg
    49141 catttgtcgt aatgcccgtt cgcacattgt ttgagatgac ccttggcgaa acgctcttcc
    49201 ttaatgccaa actgccaacc ggtaaagaat ttatgccgcg tgaaatcagt ttgttgattg
    49261 gtgaagaagg aaatccgctg agcagccagg aagtactgga gggcggtgaa tcgctgatat
    49321 tatcggaagt cgcagagcca ccagcacaaa tgattgattc tctcaccacc ttatttaaaa
    49381 ctattaagcc ggtgaagcgt gcttttattt gttcaattaa agagaacgaa gaggcacagc
    49441 ctaatttact tattggcatt gaagccgatg gtgatatcga agaaattatt caggcggcgg
    49501 gaagtgtagc gaccgataca ttacctggcg atgaacccat cgatatttgt caggtgaaaa
    49561 aaggggaaaa aggaattagc cactttatta ccgaacatat tgtgccattc tatgaacgtc
    49621 gctggggtgg ttttttgcgt gactttaaac agaaccggat aatctaaaat atgccgcccc
    49681 aaagggcggc attcgggctt acctgctgtt ctgaaggttg ttaacaacca ataaattgtt
    49741 gccggagaga ctccggttat tatttacctc ggttaacagc gcaccagaaa caataagtac
    49801 gatacaaaag caccgtatct tcattgtgcg gtccatgcga agaccgcatc acgacagcac
    49861 cactggcgat attgccgcga tagcaagcgc tgagacgcgc ttgccctaac ggtcgcggaa
    49921 catcctttcc ttgagccagc gcccgcttgc gagacggcgg aaaggtccgc agtggataat
    49981 acactaacag aacaataata actctctttt acttgacaaa gagagagtta ctggcgagta
    50041 ttgtgtccgc tgagacgcgc ttgcattact acggtaatac aatctgctgg aaaggccgcg
    50101 aaccaaacca gcaacaaata accgcctgat tttggcggtt ttttgttgtt taaagaaagt
    50161 gaagttacac gtttaactaa tatcttttat cagttagtat attttaatat taagcgggat
    50221 ttgtttatat ttgtctgaat tcggactgat aagctaaatg aattcaaaat ctatcctttt
    50281 ttatctactt gacatcgttg atgcataaag cgagtattga gtagacgaga tacacttgca
    50341 gtaatacaat ctgctggaaa ggccgcgaac cagaccagca ataaaaaacc gccaaattcg
    50401 gcggtttttt attgcctgta aaaagcttat tttacgggct caaccggtaa atctgctcgt
    50461 gcgccccatt cactccatgc gccgtcgtac agtttcacgt ttggcacatc cagcgtcgcg
    50521 agtgccaaca aaaccacggc tgccgttaca ccagagccgc agctgacgat aatcggtttg
    50581 tcatagctga cgccgcgacc aaaaaatatc gcatccagtt catcggtcgt ttttagttcg
    50641 ccttcgcgca ccagttctgt ccacggaacg ttaaacgcgc cgggaatatg tccgcgacgt
    50701 aaacctgggc gaggttcatc aacttctgcg ttaaaacgtg cagccgggcg ggcatcgata
    50761 atttgcgccg tattttcatg gcttgccaat aacacatcgg ttactttcac cacggcttca
    50821 ggattaaacg cggcgttaaa ctctccttcc ggcagctcta ctgcgccttc ttcaaatagc
    50881 agatcatcac gctgccagcc tgcaagtcca ccacccagaa tcgacacttt ctctacacca
    50941 aaggtgcgca gcatccacca tgctcgtggg gctgagaaaa gattaccttc gtcatagaca
    51001 atcaggtgct tatcctggtt aacgcctaat tcacgcatcg ccacggcgaa cgtttccggg
    51061 cgcggcagca tgtgcggaag cggggaagtg tgatcagaaa gcgcttcgat atcaaaaaac
    51121 actgcgccgg gaatatgtcc attcagatac tcctgagcaa cgttacgatc ttcctgtcca
    51181 ggcgacgcca tgcgggcatc gataatctga atttccgggt catcaatatg ttcggcgagc
    51241 cagtcggctc ctacaaacca tgtcgtggac ataggcatct ccatttttgc cggggttagg
    51301 ctaaattgtc ggatgtttag tgaaaagcga caagcaaaag aggctgattt ggctgtctga
    51361 gccacaattc tcactaaaat tcggattgta tgggcgtgat actgaattac ataacgtaaa
    51421 ttctcacgca taataattat tcgacgttgc taacgggcca ttgtggcagg gatgaaaaat
    51481 gaaaaagtta cgcgtagccg cctgcatgct aatgctggcg ctggcagggt gcgacaacaa
    51541 cgataacgcg ccaacagcgg tgaaaaaaga tgcgccttct gaagttacta aagcggcctc
    51601 ttcagaaaac gtgagttcag caaaactttc cgcgccggag cgacaaaaac tggcccaaca
    51661 gagtgccggt aaggcgctga cattgctgga tctctctgaa gttcaacttg atggcgcagc
    51721 cacgctggtg ctgacgttct ccattcctct cgacccggat caggatttct cacgcgttat
    51781 ccatgtcgtc gataagaaaa gcggcaaagt ggatggtgcc tgggagctgt cagataatct
    51841 taaagagttg cgtttacgcc acctcgaacc gaaacgtgat ttgatcgtca ctattggcaa
    51901 ggaggtcaaa gcactcaaca acgcaacctt cagtaaagat tacgaaaaaa ctataactac
    51961 ccgcgatatc caacccagcg ttggttttgc cagccgtggt tcgttgttac ctggtaaagt
    52021 tattgaaggg ctgccggtaa tggcgctcaa cgttaacaat gttgatgtta acttcttccg
    52081 tgtgaagccc gaatctctgc ctgcattcat tagccaatgg gaataccgca attcgctggc
    52141 gaactggcag tctgaaaaac tgctgcagat ggcggatctg gtttataccg gacggtttga
    52201 tctcaatcct gcgcgtaaca cccgtgaaaa attattgctg ccgctgggtg atatcaaacc
    52261 gcttcagcag gcaggcgtgt atctggctgt gatgaatcag gctggacgtt acgattacag
    52321 taatcccgcg acgctgttta cgttaagtga tatcggcgtt tcagctcacc gttatcacaa
    52381 tcgtctggat atctttaccc aaagtctgga aaacggcgcg gcccagcaag gaatagaagt
    52441 ctctttatta aatgagaaag ggcagactct gactcaggca accagtgatg ctcaggggca
    52501 tgtgcagctg gaaaatgata aaaacgcggc attactgctg gcgcgtaaag acggtcagac
    52561 aacgctactc gatttaaaac ttccggcgct ggacttagca gaatttaaca ttgctggcgc
    52621 gccaggctat agcaaacagt ttttcatgtt tggcccgcgc gatctttatc gccctggtga
    52681 aacagtcatc ctcaatggtt tattgcgtga tgcagatgga aaagcattgc ccgatcaacc
    52741 catcaagtta gacgtgatta aacccgacgg gcaggtactc aggagcgtcg ttagtcaacc
    52801 ggagaatggc ctctaccact ttacctggcc actcgatagc aatgcggcaa ccggtatgtg
    52861 gcatattcgc gctaacacgg gcgataacca gtaccggatg tgggatttcc acgtcgaaga
    52921 ttttatgcca gagcgcatgg cgctgaatct gactggtgag aaaaccccgc taacgccgaa
    52981 agatgaagtg agattctccg tggtggggta ctacctgtat ggtgcgcctg ctaatggtaa
    53041 tactttgcaa gggcaacttt tcctgcgccc actgcgtgaa gctgtgtcag ccttacctgg
    53101 tttcgaattc ggcgatatag ctgccgaaaa tctttcccgc acgctggatg aagttcagtt
    53161 gacgctggat gataaagggc gtggcgaagt ttctacagaa agccagtgga aggaaacgca
    53221 ttccccatta caggttattt tccagggtag tttgttggaa tcgggcggtc gcccggtgac
    53281 gcgccgcgct gagcaggtta tctggcctgc cgatgcattg ccggggatcc gtccgcagtt
    53341 cgcctcgaaa tcggtttacg attatcgcac tgacagcacg gtgaaacagc ccattgttga
    53401 tgaaggcagt aacgccgctt ttgacatcgt ttatagcgat gcgcaaggcg taaaaaaagc
    53461 cgtgtcgggc ttgcaggtgc gcctgattcg cgaacgccgc gattactact ggaactggtc
    53521 agaagatgaa ggctggcagt cacagtttga tcaaaaagat ctgattgaaa atgaacaaac
    53581 tctggatctg aaagcggacg aaaccggcaa ggtcagtttc ccggtagagt ggggcgctta
    53641 tcgtctggaa gtcaaagcgc cgaatgaagc ggtcagtagt gtgcgtttct gggctggcta
    53701 tagctggcag gacaacagcg acgggagcgg tgccgtgcga cccgaccgtg tcacgctgaa
    53761 actggataaa gccagttatc gccctggcga taccattaag ttgcatattg ctgcgccaac
    53821 ggcgggtaaa ggttatgcga tggtcgagtc cagtgaaggg ccgctgtggt ggcaagagat
    53881 tgatgttccg gctcaagggc tggatctgac gattccggtc gataaaacct ggaatcgtca
    53941 tgatttgtat ttaagtacgc tggtggtacg tcctggcgat aaatctcgct ctgcgacgcc
    54001 aaaacgcgca gttggggtgt tgcatctgcc gcttggcgat gaaaaccgtc gtctcgatct
    54061 ggcgctggaa acccccacca aaatgcgccc caatcagcca ttaaccgtga aaattaaagc
    54121 cagcaataaa aatggtgaga tgccaaaaca ggtgaatgtg ctggtgtctg ccgttgatag
    54181 cggtgtgctg aatattactg attacgttac ccccgatccg tggcaggcgt tctttggtca
    54241 gaaacgctat ggtgcagaca tttacgatat ttacggtcag gtcattgaag gtcaggggcg
    54301 tctggcagct ctgcgttttg gtggcgatgg tgatgagctg aaacgtggtg gtaaaccgcc
    54361 ggtcaatcac gtcaatattg tcgcgcagca ggcgctgccg gtaacgctca acgaacaggg
    54421 cgaaggctcg gttacactgc cgattggcga ttttaacggt gaactacgcg tcatggcgca
    54481 agcctggacg gcagatgact tcggtagcaa cgaaagcaaa gtgatagttg ccgcaccggt
    54541 gattgctgaa ctgaacatgc cgcgctttat ggcgagtggc gatacctcgc gtctgacgct
    54601 ggatatcact aatcttaccg ataaaccgca aaaactgaac gttgccctga ccgccagtgg
    54661 tttgcttgaa ctggtcagcg tttcacccgc agccgttgaa ttagcgccag gtgtgcgtac
    54721 tacgctgttt atcccggtgc gagcattgcc gggttatggc gatggagaaa ttcaggccac
    54781 cattagcggg ttagcgttac cgggtgaaac cgttgccgat cagcataagc agtggaaaat
    54841 cggcgtccgt ccggcgttcc cggcacaaac ggttaattac ggtacggcgt tgcagcctgg
    54901 tgagacatgg gcgattccgg cggatggatt gcaaaacttc tcgcctgtta cactggaagg
    54961 gcaattgttg ttgagcggca aaccaccgct gaacatcgca cgttatatca aagagttaaa
    55021 agcgtatccg tacggctgtc ttgagcaaac cgccagcggc ctgttcccgt cactttatac
    55081 caacgcagcc caactgcagg cgttgggcat caaaggcgac agtgatgaga agcgccgtgc
    55141 atcggtcgat atcggcattt cccgtttgct gcaaatgcaa cgtgataacg gcggctttgc
    55201 gctgtgggat aaaaacggtg acgaagagta ctggctgacg gcttacgtga tggatttcct
    55261 ggtccgcgca ggtgagcagg gttacagcgt gccgacagac gtcattaacc ggggaaatga
    55321 gcgtctgctg cgctatttac aagatccggg catgatgtcg atcccgtacg cggataatct
    55381 caaagccagt aaattcgccg tacagtctta cgctgcgctg gtgctggccc gtcagcaaaa
    55441 agcaccactg ggtgcgctgc gtgaaatctg ggagcatcgt gcagatgctg cgtctggatt
    55501 accgctgctg caacttggcg ttgcgctgaa aaccatgggg gatgcgacgc gtagtgaaga
    55561 agcgattgcg ctggcgctga aaacgccgcg taatgatgag cggaaatggc tgggtgatta
    55621 cggtagccca ctgcgcgaca acgcgttaat gctctccttg ctggaagaaa ataaactgct
    55681 acccgatgag caaaacactt tgctgaacac gctttcgcag caggcgtttg gtgaacgctg
    55741 gctatcgacg caggaaagca acgcgttgtt catggctgcc cgtacgatcc aggatttacc
    55801 gggtaaatgg caggcgcaaa cctctttctc aactgagccg ctgacaggcg agaaagcgca
    55861 aaacagcaat ctgaatagcg atcaacttgc cgccttgcag gtgaccaaca gtggcgatca
    55921 gccgttatgg ctgcgtgtgg atgccagcgg ttatccgcaa tccgcacctt taccggcgaa
    55981 caatgtgctg caaatcgagc gtcatattct tggtactgat ggtaagagca aatcgctgga
    56041 ctcgttacgt agcggcgatc tggtgctggt ctggttgcag gttaaagcca gtaacagcgt
    56101 gccggatgcg ttagtcgtgg atctgctgcc tgcgggtctg gaactggaaa atcagaatct
    56161 ggcgaacggt agcgccagcc tggagcaaag tggtggcgaa gtgcaaaact tactgaacca
    56221 gatgcagcag gcgagcatta agcacattga gttccgtgac gatcgctttg tggcggcggt
    56281 tgccgttgat gaataccaac cggtaacgct ggtgtatctg gcgcgggcgg tgacgccggg
    56341 aacgtatcag gtaccgcaac cgatggtgga atcaatgtat gttccccaat ggcgggcgac
    56401 cggcgcggct gaagatttgc tgattgtcag accgtaaatg cctcgcttgt taaccaaacg
    56461 cggctgctgg ataacgctgg cagctgcgcc atttctcctt tttcttgcag cgtggggagc
    56521 agataaactc tggcctctac cgctgcatga agtcaatccc gcacgagtgg ttgtggcgca
    56581 ggatgggacg ccgctctggc gctttgccga tgcggaaggc atctggcgtt atccggtaac
    56641 aatcgaagat gtttctccac gttaccttga agcgctgatc aattatgaag atcgctggtt
    56701 ctggaagcat ccgggggtga atccattctc ggtggcgcgc gcagcatggc aagatctcac
    56761 ttcgggaaga gttatttccg gtggcagcac gctcactatg caggttgctc gtctgcttga
    56821 tcctcacccc aaaacatttg gcggcaaaat tcgccagctc tggcgcgcgt tgcaactgga
    56881 gtggcatctg tctaagcgtg aaattctgac cttgtatctt aaccgcgctc cgtttggcgg
    56941 tacgttgcag gggatcggtg cggcaagttg ggcttatctc ggaaaatcgc ctgcgaattt
    57001 aagctattcc gaggcggcaa tgctggcggt tttgccgcaa actcccagcc gtcttcgccc
    57061 ggatcgttgg ccggagcgtg ccgaagccgc gcgtaataaa gtgctcgaac ggatggccgt
    57121 gcaaggtgtg tggtcccgtg agcaggtaaa agagtcaagg gaagaaccca tctggctggc
    57181 accccgacaa atgccgcaac tggcaccgct gttttcgcgc atgatgctcg gtaaaagcaa
    57241 aagcgacaaa atcgttacta cgctggatgc cggtcttcaa cgacgtctgg aagaactggc
    57301 gcaaaactgg aaaggacgat taccaccgcg cagctcactg gcgatgatcg tggttgatca
    57361 taccgatatg cgtgttcgcg gctgggtggg ttcggttgat ctcaacgatg attcacgctt
    57421 tggtcatgtt gatatggtca atgcgatccg atcgccagga tcggtgctca aaccgtttgt
    57481 ttatggtctg gcgctggatg aaggcttgat ccacccggca tcactgctgc aagacgttcc
    57541 ccggcgtacc ggtgattacc gacctggcaa ctttgacagc ggttttcatg gcccgatcag
    57601 catgagcgag gcgctggtgc gctcgctgaa tttacctgct gtgcaggtgc tggaagccta
    57661 tgggccgaaa cggtttgcgg caaagttacg caatgttgga ttgccgttat atttgcccaa
    57721 cggtgctgca ccgaatcttt cgctcattct cggcggcgct ggtgcaaaac tgggggatat
    57781 ggcggcggcc tataccgcgt ttgctcgcca cggcaaggca ggcaaattgc gcttacagcc
    57841 tgacgatccg ctgcttgaac ggcctttaat gtcgtcgggg gcggcgtgga tcattcgccg
    57901 gattatggct gatgaagcgc aacccttgcc ggatggtgcc ttgccgcgcg tcgccccact
    57961 ggcatggaaa acgggcacca gctatggcta tcgtgatgcc tgggcgattg gggttaacgc
    58021 tcgctatgtt attgggatct ggactggcag accggacggc acgcccgttg ttggtcagtt
    58081 tggctttgcc agcgccgtac cattgttgaa tcaggttaat aatatcttac tgtcgcgcag
    58141 tgcgaatctg ccggaagacc cacgtcctga ctctgtaagt cgtggcgtta tctgctggcc
    58201 gggcggacag tctttgccgg aaggtgacgg taactgccgc ctccgcctgg caacctggct
    58261 gctggacggg agtcagccgc caactctatt actgccggag caggaaggca ttaatggcat
    58321 tcgtttcccc atctggctgg atgaaaacgg caaacgtgtt gccgctgatt gccctcaggc
    58381 gcgacaagaa atgataaatg tctggccgct accgctggaa ccctggttgc ccgcatcaga
    58441 acaccgcgct gtgcgcttac caccggcctc gacaatttgc ccgccatacg gtcacgatgc
    58501 acaactccca ctacagctga caggtgttcg cgatggcgcg attattaaac gtttaccggg
    58561 cgcagcggaa gcgactttgc cgttgcaatc aagtggaggg gctggtgaac gctggtggtt
    58621 tctaaacggc gaaccgttaa ctgaacgcgg gcgcaacgtg accctgcatt tgacggataa
    58681 aggcgattat caattgctgg tgatggatga cgtggggcaa atcgcgacag tgaaatttgt
    58741 catgcaatag tctgttttgt ccctgattgt tgcttaaact aggtttattt taaaaaaatg
    58801 ttaccttcat caatagtcaa cggccctgtt gctcattata atccgcgcca tctcgtacgc
    58861 tggtacagac aacaacagaa caatttacag aggtaaaaat ggctattgaa cgtacttttt
    58921 ccatcatcaa accgaacgcg gtagcaaaaa acgtcattgg taatatcttt gcgcgctttg
    58981 aagctgcagg gttcaaaatt gtcggcacca aaatgctgca cctgaccgtt gaacaggctc
    59041 gtggctttta tgctgaacac gatggaaaac cgttctttga tggtctggtt gaattcatga
    59101 cctctggccc gatcgtggtt tccgtgctgg agggtgaaaa cgccgttcag cgtcaccgcg
    59161 atctgctggg cgcgaccaat ccggcaaacg cactggccgg tactctgcgt gctgattacg
    59221 ctgacagcct gaccgaaaat ggtacccacg gttctgattc cgtcgaatct gccgctcgcg
    59281 aaatcgctta tttcttcggc gaaggcgaag tgtgcccgcg cacccgttaa taatttcgta
    59341 aatgctacgt gcaaacgtgg catccgtgcg ccagaatttg tacaatgcag cgcccccgga
    59401 cgagcagccg ctcaccgggg cgtttctttt ttcaaccctc caggggccat aacgtgtaat
    59461 aacgaggccg gaatagcata tgtctgaaca attagtcaca cctgaaaacg tcaccacgaa
    59521 agatggaaaa atcaacctgc tggatctcaa ccgtcagcag atgcgggagt tttttaaaga
    59581 tttaggtgaa aaacccttcc gcgccgatca ggtgatgaag tggatgtatc actattgctg
    59641 cgacaacttt gatgagatga ccgacatcaa caaagtgttg cgcggcaaac tgaaagaggt
    59701 ggcggagatc cgcgcaccgg aagtggttga agaacagcgt tcatctgacg gcaccattaa
    59761 atgggcaatt gccgttggcg atcagcgcgt cgaaacggtg tatatcccgg aagacgaccg
    59821 tgccacgctc tgcgtctctt cgcaggtggg gtgtgcgctg gagtgtaaat tctgttccac
    59881 tgcccagcag ggctttaacc gcaacctgcg ggtatcggaa attatcggcc aggtgtggcg
    59941 tgcagcgaaa atcgtcggcg cagcaaaagt caccggtcag cgtccaatca ctaacgtagt
    60001 gatgatgggc atgggcgagc cgctgctcaa cctgaacaat gtcgttccgg cgatggaaat
    60061 catgcttgat gatttcggtt ttggcctgtc taaacgtcgc gtgacgcttt ccacttccgg
    60121 tgtagttccg gcgctggata aactgggcga tatgatcgac gttgcactgg caatttccct
    60181 gcacgcgccg aacgacgaaa ttcgtgacga aattgtgccg atcaacaaaa agtacaatat
    60241 cgaaacgttc cttgctgcgg tgcgccgtta tctggagaaa tccaacgcca atcagggccg
    60301 agtcactatt gaatacgtga tgcttgacca cgttaacgac ggcactgaac acgcgcacca
    60361 actggcggag ctactgaaag atacgccgtg taagatcaac ctgatcccgt ggaacccgtt
    60421 cccgggcgcg ccgtatggac gcagttcgaa cagccgtatc gaccgtttct caaaagtgct
    60481 gatgagctac ggtttcacca ctattgtgcg taaaactcgt ggtgatgata tcgatgctgc
    60541 ctgtggacag ttggcgggcg atgttatcga ccgtacgaaa cgtactctgc gtaaacggat
    60601 gcagggtgaa gccatcgaca ttaaagcggt ctgatagcca ttacgccacg gttactttat
    60661 gtaccgtggc gtaatctttt atgaatgaag tattaagttg caattgtctg gtcaatatta
    60721 acggtgcgtt tttgctgact ttaaggcagt atgtaacgat gcaacagtaa gttagcctta
    60781 gtgaatgtgg gctttgtcac gagcacacag acggtcttat actgtatgat aacggttaac
    60841 ttaacggatg tttcgcggtg tgggtgagca tgatggttca ccggcatctc aattctcatt
    60901 taaacgtacc tgcagcgaat gaatactgaa gccacgcacg accaaaatga agcacttact
    60961 accggcgctc gcctgcgtaa tgctcgcgaa caactaggac ttagtcagca ggccgttgcc
    61021 gagcgacttt gcctgaaggt ttccacggta cgcgacattg aagaagataa ggcacccgcc
    61081 gatcttgctt caacattcct gcgcggatat atccgctctt atgcgcgtct ggtacatatt
    61141 ccagaagaag aactgctgcc agggctggaa aagcaggctc cactacgggc tgcaaaagtt
    61201 gcgccgatgc agagtttttc cctcggtaaa cgccgcaaaa aacgcgacgg ctggctgatg
    61261 accttcacct ggctggtgtt gtttgtggtt atcggcctga gcggtgcctg gtggtggcaa
    61321 gaccacaaag ctcagcagga agagatcacc actatggccg atcaatcttc ggcggaactg
    61381 agcagtaata gcgagcaggg gcagagtgtt ccgttaaata cgtcgacaac tacagacccg
    61441 gctacaacgt ccacgccgcc agcgtctgtg gatactaccg caaccaacac gcaaacacct
    61501 gccgtaactg cgccagcacc agctgttgat ccgcaacaga atgcggttgt ttcgccttcg
    61561 caggcaaatg ttgataccgc cgcgaccccg gcaccaacgg caacaacaac gccagatggt
    61621 gctgcgccgt tgccaaccga tcaggctggc gtgaccacgc cggcggctga tccgaatgcg
    61681 ctggtgatga actttactgc cgattgctgg ctggaggtca ctgatgctac cggtaaaaaa
    61741 ctgtttagcg ggatgcagcg taaagacggt aatttgaatt taaccggcca ggcaccgtat
    61801 aaactgaaaa ttggtgcgcc agccgcagta cagatccagt atcaagggaa acctgtcgat
    61861 ctgagtcgtt ttatcagaac taaccaggtt gcgcgtctga ccctcaatgc cgaacaatca
    61921 ccggcgcagt aacagacggg taacgcggga gatttttcat gcataaccag gctccaattc
    61981 aacgtagaaa atcaacacgt atttacgttg ggaatgtgcc gattggcgat ggtgctccca
    62041 tcgccgtaca gtccatgacc aatacgcgta cgacagacgt cgaagcaacg gtcaatcaaa
    62101 tcaaggcgtt ggaacgtgtt ggcgctgata tcgtccgtgt ctcagtaccg acgatggacg
    62161 cggcagaagc gttcaaactc atcaaacagc aggttaacgt gccgctggtg gctgacatcc
    62221 acttcgacta tcgcattgcg ctgaaagtag cggaatacgg cgtcgattgt ctgcgtatta
    62281 accctggcaa tatcggtaat gaagagcgta ttcgcatggt ggttgactgt gcgcgcgata
    62341 aaaacattcc gatccgtatt ggcgttaacg ccggatcgct ggaaaaagat ctgcaagaaa
    62401 agtatggcga accgacgccg caggcgttgc tggaatccgc catgcgtcat gttgatcatc
    62461 tcgatcgctt gaacttcgaa cagttcaaag tcagcgtgaa agcgtcagac gtcttcctcg
    62521 ctgttgagtc ttatcgtttg ctggcaaaac agatcgatca gccgctgcat ctggggatca
    62581 ccgaagcggg tggtgcgcgc agcggggcgg ttaaatccgc cattggttta ggtctgctgc
    62641 tgtctgaagg cattggcgac acgctgcgcg tatcgctggc ggccgatccg gttgaagaga
    62701 tcaaagtcgg tttcgatatt ttgaaatcgc tgcgtatccg ttcgcgcggg atcaacttca
    62761 tcgcctgtcc aacctgttcg cgtcaggagt ttgatgttat cggtacggtt aacgcgctgg
    62821 agcaacgcct ggaagatatc atcactccga tggacgtttc gattatcggc tgtgtggtga
    62881 atggcccagg tgaggcgctg gtttctacac tcggcgtcac cggcggtaac aagaaaagcg
    62941 gcctctatga agatggcgtg cgcaaagacc gtctggacaa caacgatatg atcgaccagc
    63001 tggaagcgcg cattcgtgcg aaagccagtc agctggacga agcgcgtcga attgacgttc
    63061 agcaggttga aaaataataa cgtgatggga agcgcctcgc ttcccgtgta tgattgaacc
    63121 cgcatggctc ccgaaacatt gagggaagcg ttgagggttc atttttatat tcagaaagag
    63181 aataaacgtg gcaaaaaaca ttcaagccat tcgcggcatg aacgattacc tgcctggcga
    63241 aacggccatc tggcagcgca ttgaaggcac actgaaaaac gtgctcggca gctacggtta
    63301 cagtgaaatc cgcttgccga ttgtagagca gaccccgcta ttcaaacgtg cgattggtga
    63361 agtcaccgac gtggttgaaa aagagatgta cacctttgag gatcgcaatg gcgacagcct
    63421 gactctgcgc cctgaaggga cggcgggctg tgtacgcgcc ggcatcgagc atggtcttct
    63481 gtacaatcag gaacagcgtc tgtggtatat cgggccgatg ttccgtcacg agcgtccgca
    63541 gaaagggcgt tatcgtcagt tccatcagtt gggctgcgaa gttttcggtc tgcaaggccc
    63601 ggatatcgac gctgaactga ttatgctcac cgcccgctgg tggcgcgcgc tgggtatctc
    63661 cgagcacgta actcttgagc tgaactctat cggttcgctg gaagcacgcg ccaattaccg
    63721 cgatgcgctg gtggcattcc ttgagcagca taaagaaaag ctggacgaag actgcaaacg
    63781 ccgcatgtac actaacccgc tgcgcgtgct ggattcaaaa aatccggaag tgcaggcgct
    63841 tctcaacgac gctccggcat taggcgatta tctggacgag gaatctcgtg agcattttgc
    63901 cggtctgtgc aaactgctgg agagcgcggg gatcgcttac accgtaaacc agcgtctggt
    63961 gcgtggtctg gattactata accgtaccgt tttcgagtgg gtgactaaca gtctcggctc
    64021 ccagggcacc gtgtgtgcag gcggtcgtta tgacggtctg gtggaacaac tgggcggtcg
    64081 tgcaacaccg gctgtcggtt ttgcgatggg cctcgaacgt cttgtattgt tagtacaggc
    64141 cgttaatccg gaatttaaag ccgatcctgt tgtcgatata tacctggtgg cttcaggtgc
    64201 tgatacacaa tctgcggcta tggcattagc tgagcgtctg cgtgatgaat taccgggcgt
    64261 gaaattgatg accaaccacg gcggcggcaa ctttaagaaa cagtttgccc gtgctgataa
    64321 atggggtgcc cgcgttgctg tggtgctggg tgagtctgaa gtggctaacg gcacagcagt
    64381 agtgaaggat ttgcgctctg gtgagcaaac ggcagttgcg caggatagcg tagccgcgca
    64441 tttgcgcacg ttactgggtt aaggaaggag aaggacagcg tggaaattta cgagaacgaa
    64501 aacgaccagg tagaagcggt taaacgcttt tttgctgaaa atggcaaagc actggctgtt
    64561 ggggtgattt tgggcgttgg cgcactaatt ggctggcgct actggaacag ccatcaggtt
    64621 gattctgcac gctccgcttc tcttgcctat caaaatgcgg ttaccgcagt gagcgaaggc
    64681 aaaccggata gcattccggc ggcggaaaaa tttgctgctg aaaataaaaa tacttatggt
    64741 gcgctggctt ctttggaact tgcgcagcaa tttgttgaca aaaatgaact agagaaagct
    64801 gccgcccagt tacaacaggg gctggcagac acgagcgatg aaaatctcaa agccgtgata
    64861 aatctgcgtc ttgctcgcgt tcaggtacag ctcaagcagg ctgatgccgc gctgaaaacc
    64921 cttgatacca tcaaaggtga agggtgggct gccattgttg ccgacctgcg tggtgaagca
    64981 ttgctgagca aaggtgataa gcaaggtgcg cgtagtgcat gggaagcagg cgtgaaaagc
    65041 gatgttactc cggcactgag cgaaatgatg cagatgaaaa ttaataattt gtccatctga
    65101 gagggacccg atgcaattgc gtaaattact gctgccagga ctgctttccg ttaccctttt
    65161 aagcggctgt tcgctgttta acagcgaaga agatgtggta aagatgtccc cattgccaac
    65221 cgttgaaaac cagtttacgc cgaccacggc gtggagcact tccgttggta gcggcattgg
    65281 caacttctat tccaatcttc atccggcact ggcggacaac gttgtctatg cagcggaccg
    65341 cgctggttta gtaaaagcgc tgaatgcgga tgatggcaaa gaaatctggt ctgtcagcct
    65401 ggccgagaaa gatggctggt tctctaaaga gcctgcatta ctttctggcg gcgtgaccgt
    65461 gtctggcggg catgtctaca ttggcagtga aaaggcgcag gtttacgcgc tgaataccag
    65521 cgatggtact gtggcatggc aaactaaagt cgcgggtgaa gcactttcgc gtccggtggt
    65581 cagcgacggt ctggtgttaa tccacaccag taatggtcag ttacaagcgc tgaacgaaac
    65641 cgacggcgct gtcaaatgga cagttaacct cgatatgcct tcgctctctt tgcgtggcga
    65701 gtctgcgccg gcaacggctt ttggtgcggc cgtcgtgggt ggcgataatg gtcgcgtcag
    65761 cgcagtgctg atggaacagg gccagatgat ttggcagcag cgtatttccc aggcgaccgg
    65821 ttcaaccgaa attgaccgtc tgagcgatgt tgacacgact cccgtcgttg ttaacggcgt
    65881 tgttttcgcg ctggcctata atggtaacct gacggcgctt gatctgcgca gcggtcagat
    65941 tatgtggaaa cgcgaactgg gttcggtgaa tgatttcatc gtcgacggca accgcatcta
    66001 tctggtcgat caaaatgacc gggtgatggc gttgaccatt gatggcggcg ttacgctgtg
    66061 gacacaaagc gatctgttgc atcgcctgct gacttctccg gtgctgtata atggcaacct
    66121 ggtggtaggt gacagtgaag gttatctgca ctggattaac gtcgaagatg gtcgtttcgt
    66181 tgcccagcaa aaagttgata gttccggttt ccagactgaa ccggttgccg ctgacggcaa
    66241 actgctgatc caggcaaaag acggaaccgt gtactctatt acacgttaat cgtctctgtc
    66301 gttcactttg aaaacggctc ctggacaggg gccgttttcc tgtttttaac aacgacacga
    66361 atatagcgtg cgttgtctga tgatttataa aaatgaggct ttaaacatgg tacctgtggt
    66421 cgcgcttgtc gggcgcccta acgtaggaaa atccacgtta tttaaccgtc taactcgcac
    66481 ccgagatgcg ctggttgcgg atttcccggg tctgactcgt gaccgtaagt acggtcgtgc
    66541 agaaattgaa ggccgtgagt ttatctgcat tgataccggc gggattgatg gcacagaaga
    66601 cggtgtagaa acccgcatgg cggaacagtc gctgctggcg attgaagaag cggacgtcgt
    66661 actgtttatg gtggatgcgc gcgcgggcct gatgccggcg gatgaagcga ttgctaaaca
    66721 tctgcgctcc cgtgaaaaac cgaccttcct ggtggcaaac aaaactgacg gtctggatcc
    66781 cgatcaggca gtggttgatt tctactcgct tggtttaggg gaaatctacc cgatcgccgc
    66841 gtctcacggt cgtggcgtat taagtctgct tgagcacgtg ctgctgccgt ggatggaaga
    66901 tctcgcaccg caagaagaag tcgacgaaga cgctgaatac tgggcgcaat ttgaagcaga
    66961 agaaaacggc gaagaagaag aggaagatga cttcgacccg caaagtctgc cgatcaaact
    67021 ggcgattgtg ggtcgtccga acgtaggtaa gtctacactc actaaccgta ttcttggtga
    67081 agagcgcgtt gttgtttacg acatgcctgg cacgacgcgt gacagcatct acattccaat
    67141 ggagcgcgat ggacgtgagt atgtactcat tgacaccgct ggcgtacgta aacgcggcaa
    67201 aatcaccgat gctgtagaga aattctcggt aatcaaaacg ttgcaggcca ttgaagatgc
    67261 caacgtggtg atgttggtga ttgacgcccg cgaaggtatt tccgatcagg atctctcgct
    67321 gctgggcttt attctcaata gtgggcgctc acttgtcatt gtggtgaata agtgggatgg
    67381 cctgagccag gaagtgaaag agcaggtgaa agaaacgctg gacttccgtc tgggctttat
    67441 cgattttgct cgtgtgcact ttatctctgc cttgcacggc agtggtgttg gtaacctgtt
    67501 tgaatcagtg cgtgaagcgt atgacagctc tacccgtcgc gtgggtacgt ctatgctgac
    67561 ccgcatcatg accatggctg ttgaagatca ccagccgccg ctggtacgcg ggcgtcgcgt
    67621 gaagctgaaa tatgcccacg ccggtggtta taacccgccg attgtggtga ttcacggtaa
    67681 ccaggtaaaa gacctgccgg attcctataa gcgttatctg atgaactatt tccgcaagtc
    67741 tctggatgtg atggggacgc cgattcgtat tcagttcaaa gaaggggaaa acccgtatgc
    67801 gaacaagcgt aacaccctga cgccgaccca gatgcgcaag cgtaagcgcc tgatgaagca
    67861 tatcaagaaa agtaagtaat cacttatttg gtttcccttt ccacacggaa agggaaaccg
    67921 aataacgcgc gaggagtatt tatggcactc aactgtccaa cctgcaaaac ccctctcgaa
    67981 cgcaatggcg agactgccca ctgtgcaact tgcgacaaag actttaccgt ccaggcactt
    68041 tgccctgagt gtcatcagcc gttacaagtg ttaaaagctt gtggtgctgt ggactatttc
    68101 tgccaacatg ggcacggttt aatatctaaa aaacgcgttg aatttgttat tgcttaatat
    68161 catgtgaata tattgtttta tcatttatat ttaatatgtg aatggtaaaa caatctg
//
LOCUS       DAPOZO010000011        67187 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPOZO010000011 DAPOZO010000000
VERSION     DAPOZO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.011"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(154..639)
                     /gene="sixA"
                     /locus_tag="ROI21_000762"
     CDS             complement(154..639)
                     /gene="sixA"
                     /locus_tag="ROI21_000762"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDX5109727.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(842..2986)
                     /gene="fadJ"
                     /locus_tag="ROI21_000763"
     CDS             complement(842..2986)
                     /gene="fadJ"
                     /locus_tag="ROI21_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDX5109728.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRMCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            complement(2986..4296)
                     /gene="fadI"
                     /locus_tag="ROI21_000764"
     CDS             complement(2986..4296)
                     /gene="fadI"
                     /locus_tag="ROI21_000764"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDX5109729.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(4477..4761)
                     /locus_tag="ROI21_000765"
     CDS             complement(4477..4761)
                     /locus_tag="ROI21_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDX5109730.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5133..6473
                     /gene="fadL"
                     /locus_tag="ROI21_000766"
     CDS             5133..6473
                     /gene="fadL"
                     /locus_tag="ROI21_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDX5109731.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSDRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGKTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6839..8086
                     /locus_tag="ROI21_000767"
     CDS             6839..8086
                     /locus_tag="ROI21_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109732.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEVNGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAMRVNDNPDRFNINNSSVTYINIDSNDTDYITIGIDNQEPIKLLAS
                     YKDKELARTIINDNIVEKTHDDINNAEFTDGSIVVSGNEGAIDSNDTADITSGIYNQE
                     PIKSPTNDENKKVGQTIIHENVVENTHDINNKEMIFSALKEIYDGDPGFIFDKISQKL
                     RHTITEFDENGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQR
                     GIRINGDSLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEV
                     FKRLGRAIRYNFQVDDAKFRRDNVKEIISTLVDNEVDRSQNKYDHFKEIEDKVEKRLQ
                     NRQAKYQNEINQLSALGVNFDDN"
     gene            complement(8265..9020)
                     /gene="mlaA"
                     /locus_tag="ROI21_000768"
     CDS             complement(8265..9020)
                     /gene="mlaA"
                     /locus_tag="ROI21_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDX5109733.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            9314..10246
                     /locus_tag="ROI21_000769"
     CDS             9314..10246
                     /locus_tag="ROI21_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDX5109734.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            10322..10396
                     /locus_tag="ROI21_000770"
     tRNA            10322..10396
                     /locus_tag="ROI21_000770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10355..10357,aa:Arg,seq:cct)
     gene            10561..11847
                     /locus_tag="ROI21_000771"
     CDS             10561..11847
                     /locus_tag="ROI21_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDX5109735.1"
                     /translation="MSRNTLNKLTDRQCKAAKPRDKTYKLSDGGGLYLEVSQTGSKYW
                     RMKYRRPSDKKEDRLAFGVYPTISLQDAREKRDNAKKLLVKGIDPKAEQRAAKAEEKG
                     AFTFETIGRQWVESHQMWNEDHRKRVLRSLEMYIFPHIGTSDIRKLEAMTILPLFKKV
                     DDAGKHDTANRLKQRVKDIIHSARLSGIKTEIITNDLDVRLMQYETKHHAALHPRDLP
                     DFFTRLGSFKGNPLTRLAIELTMLTFVRSSELRFARWKEFDLDRAEWIIPKKREPIDG
                     VRFSTRGTKTGKDEHIVLLSRQAVSIVKQLRELSGSYDVVFPNERNTKGVMSENTVNK
                     ALRLMGYNTQEDVTGHGFRATACSSLMESGLWQEDAVERQMSHKEYKDVKRAYKHKAD
                     YLEERKLMLQWWADYLDANREIHISPYEFGRRTRRD"
     gene            11840..12595
                     /locus_tag="ROI21_000772"
     CDS             11840..12595
                     /locus_tag="ROI21_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109736.1"
                     /translation="MTKMTGLDAFYNESEDKIWFGNLISELARLNNENERMIAAAILS
                     NYRLSKAKPDTLGGLGFYQFDWVSGFTTNDDFEKQCVEFLGLLAMGHEYRESPIPGEE
                     GTFNVRKGEDFLYHDDEDDWYGSYDSFYFKRSEVVNFFPELDMNVPDNEPEIGALSKK
                     VEREQNWRGKDTALKMIAGMAIALFESSKDFHSGGNLSINRVSAQVIENNIIFGNDED
                     SDTHPDTFRRLLTNALDKYAPKFKKKATKKRPK"
     gene            12752..12943
                     /locus_tag="ROI21_000773"
     CDS             12752..12943
                     /locus_tag="ROI21_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDX5109737.1"
                     /translation="MKKSLIRLSEVLKRTGYSKAWVYALMSRGQFPQSVKIGARAIAF
                     IESEIDDWIDQRIAESRSN"
     gene            12992..13183
                     /locus_tag="ROI21_000774"
     CDS             12992..13183
                     /locus_tag="ROI21_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109738.1"
                     /translation="MQLKPTNQKLNVTHVDFETFAALIRSEPQKVSTSTWVYDVKSTS
                     GGKAIGIASGDDYLVIDLI"
     gene            13265..13459
                     /locus_tag="ROI21_000775"
     CDS             13265..13459
                     /locus_tag="ROI21_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDX5109739.1"
                     /translation="MINYLTAKDAAEYLGVCLSRFYQLKRYIRSFPPYINQTINGRKR
                     RVWLASSLDQFADRFLGGRK"
     gene            13456..13842
                     /locus_tag="ROI21_000776"
     CDS             13456..13842
                     /locus_tag="ROI21_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109740.1"
                     /translation="MSTLTRSQVAANIRDILLSGRKLTPKEFDDILRKAGNHERSRVL
                     TLLRNDWGIPVEQFKTEAYHVTERNLEAYHSDKDETLKIWRTNARYVKTLRKVNITLS
                     LLRGLVGKVPEDTLRTVYKGIETKYL"
     gene            14316..14963
                     /locus_tag="ROI21_000777"
     CDS             14316..14963
                     /locus_tag="ROI21_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDX5109741.1"
                     /translation="MVNERSINGQAGKIVSKWVTLANNAIPSNVPPQWDLDLKGQQYS
                     EPTRCIGKQIDYMAKPETKLNDFNREASKSLYDWSDANVWGYSNGWARHEIKKETLSI
                     TGFHAVRRILKAIQASRKDNYKVKRQIQRTLKRQDINRSPITPFQAPLISKHDWDLSV
                     IAVTKEAETERRELLSSQTINSNKVQTHPLLRMLRKARVRASRARRESGGYDHYY"
     gene            complement(15762..16754)
                     /locus_tag="ROI21_000778"
     CDS             complement(15762..16754)
                     /locus_tag="ROI21_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109742.1"
                     /translation="MKIIKNIPLYGYSVDPEQLSFVDRLLTKSKNERPGFYQRMFYED
                     FARVFNFVNHGDANLFQVETNDEELIRKMFGNLQARYRKYSVDETIRELVEAVAQSLI
                     WQGTAYYFVQNVPEQEKIHITPLVSDNIFCFFSVYFQYVPKRCERYLERDHEMLPREL
                     RILDKNKLMRFEIPRSLRRMLSEQNRTLKIIDKHQFGVTNFYPQATYENPNPKSHFDF
                     SIWKNTQEQALFRATRKTGWNGRNYDSSKCSDFFHYYRLIRFRRNQLILRDYILLQLG
                     KELTRVGHRYNEDFNVVISPTSVLPQIDKLDEMESLLSREEIDFTEVSDYYYER"
     gene            complement(16751..19216)
                     /locus_tag="ROI21_000779"
     CDS             complement(16751..19216)
                     /locus_tag="ROI21_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109743.1"
                     /translation="MKVWLISQKWFWKLRTDLVRVRFILERASYIHRTSVFKAKSNAH
                     VFMSFALVGLKNLLWVILTLFALGFVEDYIRNNTILLHPLSYDEKKFNIDQLRLYAQI
                     LTAIFSIYFATIGIILSTGYTRLRRDIIQMLTTEQVGNVYSRTLVLSAVFCLSATALH
                     LFGVEPGLFVYAMGTLLTLVSSLALFPLGQRLFNFFDLNQLARSEILPRIARHIEGAS
                     NPKNSVSLANHHSKAAHLAFEQLNYIDDRLICEKVGLGDNLPALSNVYSALLLHYLNQ
                     KHRIDYQSYWFPRRIKHKQWFLAGDSVTSIALKTRSQLTVEEEPNYLWLENEIINRLV
                     GHIELAFKAGDFKLGLDLISLFSSRILTYAQQFQFDIGIRELRKMKTIIEKAFTPSDV
                     VNYFEEKVIKVGIADAWAALGSNLCLEMLRRMITFEKELEQFFKDDIWTETSLHTLPA
                     FLQVELAFIVERIEFEKEIEGQRLSKPKYVQQLAVQKLLQQYSKILPEVCDFHVNMVP
                     DFVNSLINMKMPEAATQVVLASLHNHLKFPHWFNELSLLLERYRKYEHYSEKLYALPE
                     INIAEMLKQFVVTQDEAIAILGRVEIVGHIIEQEQDDELPDHFGQIYFELAEECIHAL
                     EQNDENKLSKVFPMFMVLSLLASDTKFVDPKLDINNEFRLHLLSSAINDLMSVLGFAI
                     LYSAYFDNAKLSKTVLDRFKSWLESVPNKQQYLKRMMILSNPRNFSLCASPRDMIRLN
                     WKMEFERRARHDGFSDQMGMNRGKSHTNKVVREFLNSHSDASHLFCALEIFPQLEQID
                     FKIDYHVSELARRFGVEYDKDFE"
     gene            complement(19442..19618)
                     /locus_tag="ROI21_000780"
     CDS             complement(19442..19618)
                     /locus_tag="ROI21_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109744.1"
                     /translation="MEKDLKALREYLLLSPDYPEVNDSLANFSTEDMTNEEIAERLGI
                     APDWMETVDHSKLT"
     gene            19921..20547
                     /locus_tag="ROI21_000781"
     CDS             19921..20547
                     /locus_tag="ROI21_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDX5109745.1"
                     /translation="MNVRIYCRASTEGQHADRALTSLREFSKSKGWQIAGEYIENASG
                     AKLERVELMRLLSEAQSGDLLLVEAIDRLSRLEHSAWVELKDTLNRKGLIIVSMDLPT
                     SWQMVEMAGNDLTSGILRAVNAMLIDILATMARQDYETRRKRQQQGIERAKSEGIYIG
                     REKNQEAREIVREMLEQGVKPELIMKAAGISRATYYRIKSELSVVKSE"
     gene            20913..21065
                     /locus_tag="ROI21_000782"
     CDS             20913..21065
                     /locus_tag="ROI21_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109746.1"
                     /translation="MHSLMNSQARDTRDRSLNDDNVLEISEPRIRSTFERLYQNMNEA
                     FGLGDY"
     gene            complement(21145..22392)
                     /locus_tag="ROI21_000783"
     CDS             complement(21145..22392)
                     /locus_tag="ROI21_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5109747.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(22454..23377)
                     /locus_tag="ROI21_000784"
     CDS             complement(22454..23377)
                     /locus_tag="ROI21_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDX5109748.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLFRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     INLAQRCGALAVTAKGAMTALPCRQELEGEK"
     gene            23593..25026
                     /gene="cscA"
                     /locus_tag="ROI21_000785"
     CDS             23593..25026
                     /gene="cscA"
                     /locus_tag="ROI21_000785"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDX5109749.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHKPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(25034..26092)
                     /locus_tag="ROI21_000786"
     CDS             complement(25034..26092)
                     /locus_tag="ROI21_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDX5109750.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEDR
                     EGGRVTRIPCPLLIRCST"
     gene            26272..26490
                     /locus_tag="ROI21_000787"
                     /pseudo
     CDS             26272..26490
                     /locus_tag="ROI21_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            26508..27836
                     /gene="dsdA"
                     /locus_tag="ROI21_000788"
     CDS             26508..27836
                     /gene="dsdA"
                     /locus_tag="ROI21_000788"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDX5109751.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALQETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAAYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQSRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(27944..29482)
                     /gene="emrY"
                     /locus_tag="ROI21_000789"
     CDS             complement(27944..29482)
                     /gene="emrY"
                     /locus_tag="ROI21_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDX5109752.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGLI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(29482..30645)
                     /gene="emrK"
                     /locus_tag="ROI21_000790"
     CDS             complement(29482..30645)
                     /gene="emrK"
                     /locus_tag="ROI21_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDX5109753.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            31061..31675
                     /gene="evgA"
                     /locus_tag="ROI21_000791"
     CDS             31061..31675
                     /gene="evgA"
                     /locus_tag="ROI21_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDX5109754.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            31680..35273
                     /gene="evgS"
                     /locus_tag="ROI21_000792"
     CDS             31680..35273
                     /gene="evgS"
                     /locus_tag="ROI21_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDX5109755.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALTEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMSPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKDSVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPEHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     DIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     LGDLSLESHPGIGTTFTITIPVEITQQVATVEAKAEQPMTLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLNVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTAKDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINLSHQLEITPVSDDSKPEILKLLNSVKEHIAELDQEIAVFCQQND"
     gene            complement(35329..36474)
                     /gene="yfdE"
                     /locus_tag="ROI21_000793"
     CDS             complement(35329..36474)
                     /gene="yfdE"
                     /locus_tag="ROI21_000793"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDX5109756.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYS
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(36548..37492)
                     /gene="yfdV"
                     /locus_tag="ROI21_000794"
     CDS             complement(36548..37492)
                     /gene="yfdV"
                     /locus_tag="ROI21_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDX5109757.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(37561..39255)
                     /gene="oxc"
                     /locus_tag="ROI21_000795"
     CDS             complement(37561..39255)
                     /gene="oxc"
                     /locus_tag="ROI21_000795"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDX5109758.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAATARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(39309..40559)
                     /gene="frc"
                     /locus_tag="ROI21_000796"
     CDS             complement(39309..40559)
                     /gene="frc"
                     /locus_tag="ROI21_000796"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDX5109759.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(41071..41703)
                     /locus_tag="ROI21_000797"
     CDS             complement(41071..41703)
                     /locus_tag="ROI21_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDX5109760.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASIGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            41999..42274
                     /locus_tag="ROI21_000798"
     CDS             41999..42274
                     /locus_tag="ROI21_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109761.1"
                     /translation="MKVNLMLFSLFLLVSIMACNVFAFSISGGGSETSYKETEKTSVM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITPPFPTKANYLIHPVR"
     gene            complement(42351..42593)
                     /locus_tag="ROI21_000799"
     CDS             complement(42351..42593)
                     /locus_tag="ROI21_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDX5109762.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            42946..43866
                     /gene="lpxP"
                     /locus_tag="ROI21_000800"
     CDS             42946..43866
                     /gene="lpxP"
                     /locus_tag="ROI21_000800"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDX5109763.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            44222..44293
                     /gene="ypdK"
                     /locus_tag="ROI21_000801"
     CDS             44222..44293
                     /gene="ypdK"
                     /locus_tag="ROI21_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDX5109764.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(44358..45596)
                     /gene="alaC"
                     /locus_tag="ROI21_000802"
     CDS             complement(44358..45596)
                     /gene="alaC"
                     /locus_tag="ROI21_000802"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDX5109765.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            45973..47670
                     /gene="ypdA"
                     /locus_tag="ROI21_000803"
     CDS             45973..47670
                     /gene="ypdA"
                     /locus_tag="ROI21_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDX5109766.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            47685..48419
                     /gene="ypdB"
                     /locus_tag="ROI21_000804"
     CDS             47685..48419
                     /gene="ypdB"
                     /locus_tag="ROI21_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDX5109767.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            48432..49289
                     /locus_tag="ROI21_000805"
     CDS             48432..49289
                     /locus_tag="ROI21_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX5109768.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRSDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFRG"
     gene            complement(49292..51787)
                     /gene="ptsP"
                     /locus_tag="ROI21_000806"
     CDS             complement(49292..51787)
                     /gene="ptsP"
                     /locus_tag="ROI21_000806"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDX5109769.1"
                     /translation="MLTIQFLCPLPNGIHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAVSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(51812..52849)
                     /gene="ypdE"
                     /locus_tag="ROI21_000807"
     CDS             complement(51812..52849)
                     /gene="ypdE"
                     /locus_tag="ROI21_000807"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDX5109770.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSTMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGKTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(52849..53934)
                     /gene="ypdF"
                     /locus_tag="ROI21_000808"
     CDS             complement(52849..53934)
                     /gene="ypdF"
                     /locus_tag="ROI21_000808"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDX5109771.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVCW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(53949..55196)
                     /locus_tag="ROI21_000809"
     CDS             complement(53949..55196)
                     /locus_tag="ROI21_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDX5109772.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(55218..55544)
                     /locus_tag="ROI21_000810"
     CDS             complement(55218..55544)
                     /locus_tag="ROI21_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDX5109773.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(55763..56728)
                     /gene="glk"
                     /locus_tag="ROI21_000811"
     CDS             complement(55763..56728)
                     /gene="glk"
                     /locus_tag="ROI21_000811"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDX5109774.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            56932..58188
                     /locus_tag="ROI21_000812"
     CDS             56932..58188
                     /locus_tag="ROI21_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDX5109775.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            58303..58629
                     /locus_tag="ROI21_000813"
     CDS             58303..58629
                     /locus_tag="ROI21_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDX5109776.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(58771..60009)
                     /locus_tag="ROI21_000814"
     CDS             complement(58771..60009)
                     /locus_tag="ROI21_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDX5109777.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            60345..61547
                     /gene="nupC"
                     /locus_tag="ROI21_000815"
     CDS             60345..61547
                     /gene="nupC"
                     /locus_tag="ROI21_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDX5109778.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(61598..63787)
                     /locus_tag="ROI21_000816"
     CDS             complement(61598..63787)
                     /locus_tag="ROI21_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDX5109779.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(63995..64070)
                     /locus_tag="ROI21_000817"
     tRNA            complement(63995..64070)
                     /locus_tag="ROI21_000817"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64035..64037),aa:Ala,seq:ggc)
     gene            complement(64110..64185)
                     /locus_tag="ROI21_000818"
     tRNA            complement(64110..64185)
                     /locus_tag="ROI21_000818"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64150..64152),aa:Ala,seq:ggc)
     gene            64406..64765
                     /locus_tag="ROI21_000819"
     CDS             64406..64765
                     /locus_tag="ROI21_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDX5109780.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            64767..65159
                     /locus_tag="ROI21_000820"
     CDS             64767..65159
                     /locus_tag="ROI21_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDX5109781.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRHFLARLDIDESE"
     gene            complement(65210..66625)
                     /gene="gltX"
                     /locus_tag="ROI21_000821"
     CDS             complement(65210..66625)
                     /gene="gltX"
                     /locus_tag="ROI21_000821"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDX5109782.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTTENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            66884..66959
                     /locus_tag="ROI21_000822"
     tRNA            66884..66959
                     /locus_tag="ROI21_000822"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:66917..66919,aa:Val,seq:tac)
     gene            67004..67079
                     /locus_tag="ROI21_000823"
     tRNA            67004..67079
                     /locus_tag="ROI21_000823"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:67037..67039,aa:Val,seq:tac)
BASE COUNT        18571 a        16300 c        15350 g        16966 t
ORIGIN      
        1 aatttttaca aataaaagcg tcatgccgta taaaataaag accattcgat cacaagcgga
       61 tcattgttag tacaataaga ttgcataaat tcaacattgc cagaaaaaaa taaaggcccg
      121 atagattaca ccagcgggcc ttgtgcatgc gtattaaatc gcttttgcca tcttcagatt
      181 acacgggctc atctgccagt tgaatgtgcc gtttccgctt tcatcaagcg ttacgctggc
      241 aatggccgag gtggtaaaca taggcggcgt ttcgcccgga cataactcag caaccagata
      301 cccgactaag ggtaagtggg agatcaccag taccgaagca acaccttcat tggtcaacgc
      361 ctgtaaatag gcactgacca gaccaacatc gccgcagggc gttaactccg gcagaacttc
      421 tgcactggaa ggcaggttca gacaatcccc tacttcttcc agtgtttgct cggctcgcag
      481 gaacgggctc accagaacgc gttcgatttc cactttttga cctttcagcc agttcgccat
      541 caggcgagat tcgtcacaac cgttagtagt cagaggacga accgaatcac tggcggcatc
      601 gagggctgcg tcgccgtgac gcatgataaa aacttgcata ttgcaccgct tttgttaacc
      661 agattcgccc gttttctttc atctgaaacc agagaaagaa aacggtggcc ggcattgtgc
      721 cttatccatt caccgaatga aacgctgttt tttacctcaa tggcgtaagt atagtcaata
      781 tgcgtttaca ttttgagcaa cactccgcca ttcagcgcgg attcatatag ctttgacctt
      841 cttattgcag gtcagttgca gttgttttcc aaaaactttc cccacgcttg ctcatctcga
      901 ctaaacgctc gcaaggggta aaacgggaac catactgcgt ggccagtcgt tgcattattg
      961 caaccacttc acccgcaccg agagaatcga tgtagcggaa cggaccaccg agaaatggcg
     1021 gaaaaccaat gccaaatacc gcgccaatat ctccgtcacg cacgctacgg ataacctgct
     1081 cgtccagaca acgtactgct tcattcagca tcaacatcac acaccgttca gctacctgcg
     1141 gtgcggagag tcgcccctgc ccttgtgtgc caatcagcgg gtaaatggcg ggatcgacct
     1201 gttttttgct tttacgccct ttctgaccat aaagatagaa accccgacca ttttttctgc
     1261 ctttgcgatc gtcgttcaaa attgaagaaa caacatttgc aggcgcgcta aaacgttctc
     1321 cataagcggc ttccagtaca ggaataattt tagtcccggt gtcgattcct acctcatcca
     1381 aaagttggat tgggcctacc ggaaaaccaa atttcaccag cgcggcatca atgtgctcta
     1441 cccgttcacc ttcggtcaac atgcggatag cttcattaat gtaaggcgct aaaatgcgat
     1501 tgacgtaaaa cccggctttg tcacgcacga caatgggcgt tttaccctgt tttttcgcca
     1561 gttttactgt ggtagcgatg gtttgcgccg atgtccccgc atgaggaata atctccacca
     1621 gcggcatttt ttccaccgga ctgaagaaat gcaggccaat aacttgctca ggtcgcgtag
     1681 cgtgagcggc gatatcacca atcggtaaag atgacgtatt cgaagcaaag atggtatgag
     1741 cggcgcaatt ttgctcaact tccgccacca tttgttgttt taattcgaga ttttcaaaca
     1801 ccgcttcaat aatcagatcg cgatgggcaa agccgcgata gtccgtcgtt ccggagatta
     1861 atgccagctg tctgtcacgt tcgctggctt tgagatgacg acggcgaact ttgccctcca
     1921 gctgatccca actgtacttc agcgcatgat ttatgccctg cgggttgata tctttaattc
     1981 tgaccggaag ccccgcttta caagcagtga cgtaagcaat accgccgccc atcaagccac
     2041 cacctaaaat tcccacgctg tttaatggcg caggcggcgc atcactgcca ggatctttct
     2101 tcacttccgt actggcaaaa aagatactac gcagcgcctg cgattgcggc gtcatcgcca
     2161 gttcgccaaa agcacgggct tcagcgtcat agccgctact ggtgccttgc gctaatcccg
     2221 tttcgacaac ctccaggatg cgttctgtcg ccggatagtt accctgagtt ttgtgttctg
     2281 ttttcttgcc gaccattttg aacagcagcg cacgacctaa cggccccgcc agaatacgct
     2341 cgcgtacagg tagagggcgt gaagatggac gatcctgctt tgccagctca acagcggctt
     2401 ccagtaaaat ggattgcgga acgacgtcat ccaccagccc cagctttaat gcctgtttcg
     2461 cccgaagttg ttttccggtg aggatcatct ctaatgctgt gctgacgcct atcagacgcg
     2521 gtaaacgctg ggtgccgcct gaaccgggta ataatccaag ttgtacttca ggcaaaccga
     2581 gcaccgtttt aggatcgtca gtacacatgc gaccgtggca cgccagcgcc aactccagcc
     2641 caccgcccag gcaagcgcca tgaatagccg cgataaccgg aacgggcaaa gcatgaatct
     2701 ccgccattaa ctgttgaccc tgccgcgcca gtgcttccgc ttcttgcgcc gttttgcagt
     2761 tggcgatcat gttgatgtct gcgccagcaa tgaagttgtc tggtttcgcg gagataaaca
     2821 ccacgccgcg caactctttg ttttcacgga gttgcttaat aatggcgcgc acctgcgagg
     2881 caaactccgc cttcagggta ttcattttct cacccggtac gtcgatggtg ataacagcaa
     2941 tgttgtccag acggacatta agggtaaacg ctgatgccat ttccattatt ccgcctccac
     3001 aaccattgcc gcgccaagcc cacccgcagc acaggcggtc actaatccaa atccaccgcc
     3061 gcgacgccga agttcatgca atgtctgggt aatcatccgc gcgccggtcg ccgcgaaggg
     3121 atgcccgtaa gcaatcgaac cgccaagcac gttaaatttg ctatcgtcca cttcgccagt
     3181 ggcatgtgca cgccccagta catcacgagc aaaacgttcg ctacccagca actgaatatt
     3241 cgccagtgtc tgagctgcaa aggcttcgtg catatcgatc aatgtcagat cgctcattgt
     3301 caaaccggca cgttccagcg ccagcggcgt tgaccaggcc ggaccgagca acatgtcctg
     3361 ccagacatct atcgcagtaa atgcgtaact gcgcagatac cccagcggta ccagccctaa
     3421 ttctttcgcc cgggattcag tcatcaggat caccgctgcc gcgccatcgg tcagcggcgt
     3481 actgtttgcc gccgttaccg ttccgtgttt gcgatcaaac gccgggcgca gctttgcgta
     3541 atcggcaagc gaggaattac cgcgaatatt gttgtcttcg acaagcggtt gtttataagg
     3601 agggataaag gcagtcatca cctcttcttt gagtttaccg tctgaccacg cctgagcggc
     3661 tcgctgatgc gaacggtgcg ctaatgcatc ttgctgttcg cgagtaatac cgtaggtttt
     3721 cgccatttgt tcagcggtgt cgcccatccg taagccagta gagtattccg ccactgccgg
     3781 aggcaccggc attaagtcgc gcaaacgcag gcgagagaag agtttcaggc gctggctcat
     3841 ggttcgagct ttgttgacat caaccagcac gcgcgccagt tttttactga cgccaattgg
     3901 caatacggaa gaggaatcgg ccccaccggc aatccccgcg cgaatagttc ccgccatcag
     3961 gctttctgcg acgtttgcaa tcgcctggaa actggtagcg caagcgcggc tgacgctgta
     4021 agcatcggta tgcacattca ttccggtgcc gagaacaatt tcacgcgcaa tgttgggggc
     4081 ttccggcatt tgtacgacct gaccaaaaac cagttgttca atcacttcgg cggggatctc
     4141 gctgcgtgcc agcagttcgc ctaccaccat cttccctaaa tcaaccgcgg gaatgccatg
     4201 aaaagccgtc gcctggcggg caaaaggcgt acgtaaaccg ctaacaatgg cgatacgatc
     4261 gccctggcgg gtaaccagcg gtaaaacctg acccataaca ctcccctgta aaataataaa
     4321 taaagtggtc tgacctgatc atagtcttaa ctattttttt acatttagcc aagtggagaa
     4381 aagggaaagt gggagctatg acacagagag aaaagtagaa gagaaaataa aacgcccctg
     4441 ccaacaactg acaggggcgt acgtacccga gaggaattaa cgcagaccca gctggaagat
     4501 cagcatttca gcttcgcagg caaaagtgaa atcgatatcc aggcgcacac cgtcagactc
     4561 ttcagtgaaa gtcggggtga ttttgcacgg ttcggattcc acgctacggg ctttttcagt
     4621 cagcgccgcc agcgtttgtt ctgcttctgc gcggtttgca aacacgcggc tgtaagacgc
     4681 ggtgcagtcg gagttgtcca taatggtgcc aacatccata cagcagcaaa ccggggtttc
     4741 atcagcacta catttactca tcgttgattt cctctgtatg tgcacccaag gtgccagata
     4801 aacgttgtgg atattttacg cttccggaaa gtgctgctcc agttgttaat tctgcaaaat
     4861 cggataagtg accgaaatca cacttaaaaa tgatctaaaa caaaattcac ccgaatccat
     4921 gagtgcgcca cctccaaatt ttgccagctg gatcgcgttt cttagatcat atttgaaaaa
     4981 agatagaaac atacttgcaa cattccagct ggtccgacct atactctcgc cactggtctg
     5041 atttctaaga tgtacctcag accctacact tcgcgctcct gttacagcac gtaacatagt
     5101 ttgtataaaa ataaatcatt gaggttatgg tcatgagcca gaaaaccctg tttacaaagt
     5161 ctgctctcgc agtcgcagtg gcacttatct ccacccaggc ctggtcggca ggctttcagt
     5221 taaacgaatt ttcttcctct ggcctgggcc gggcttattc aggggaaggt gcaattgccg
     5281 atgatgcagg taacgttagc cgtaaccccg cattgattac catgtttgac cgcccgacat
     5341 tttctgcggg tgcggtttat attgatccgg atgtaaatat cagcggaacg tctccatctg
     5401 atcgtagcct gaaagccgat aacatcgcgc ctacggcatg ggttccgaac atgcactttg
     5461 ttgcaccgat taacgaccaa tttggttggg gcgcttctat tacctctaac tatggcctgg
     5521 caacagagtt taacgatact tatgcaggcg gctctgtcgg gggtacaacc gaccttgaaa
     5581 ccatgaacct gaacttaagc ggtgcgtatc gcttaaataa tgcatggagc tttggtcttg
     5641 gtttcaacgc cgtctacgct cgcgcgaaga ttgaacgttt cgcaggcgat ctggggcagc
     5701 tggttgccgg tcagatcatg caatctcctg ccgggaagac tcctcaaggg caagcattgg
     5761 cagctaccgc caacggtatc gacagtaata ccaaaatcgc tcatctgaac ggtaaccagt
     5821 ggggctttgg ctggaacgcc ggtatcctgt atgaactgga taaaaataac cgctatgcac
     5881 tgacctaccg ttctgaagtg aaaattgact tcaaaggtaa ctacagcagc gatcttaatc
     5941 gtgcgtttaa taactacggt ttaccaatac ctaccgcgac aggtggcgca acgcaatcgg
     6001 gttatctgac gctgaacctg cctgaaatgt gggaagtgtc gggttataac cgtgttgatc
     6061 cgcagtgggc gattcactat agcctggctt acaccagctg gagtcagttc cagcagctga
     6121 aagcgacctc aacgagtggc gacacgctgt tccagaaaca tgaaggcttt aaagatgctt
     6181 accgcatcgc gttgggaacc acttattact acgatgataa ctggaccttc cgtaccggta
     6241 tcgcctttga tgacagccca gttccggcac agaatcgttc tatctccatt ccggaccagg
     6301 accgtttctg gctgagtgca ggtacgactt acgcatttaa taaagatgct tcagtcgatg
     6361 ttggtgtttc ttatatgcac ggtcagagcg tgaaaattaa cgaaggccca taccagttcg
     6421 agtctgaagg taaagcctgg ctgttcggta ctaactttaa ctacgcgttc tgataacgcg
     6481 ttcgcctgga taaagtcacc agctatgcag gtgactttaa ctcccccact tcaccgaaag
     6541 tagtttcctc attgcttgcc tcctgagttt tgcaaacagc ctgatggcag cttgcccttt
     6601 caaatcagta agtggtatcc acagacaaac ctgaagggaa acgcatattt tcaggcgttc
     6661 tgctcgtcct tctcaaagag tttacttttc tgcatttcca ggatactcaa cctccctggc
     6721 tattgtgcgc tcatacactc aaattaaaga taggctctaa ataaatgagc gttttttgat
     6781 agtctatttt attaggtaat atatatttgt aacaaatcaa tcaacatgga ataaaatcat
     6841 gctaccatct atttcaatca acaataccag cgcagcttac ccagaatcca tcaatgaaaa
     6901 taacaatgat gaagttaatg gattagtaca agagttcaaa aaccttttta atggtaagga
     6961 aggaataagc acctgtatta aacatttgct tgagcttata aaaaacgcca tgcgagtaaa
     7021 cgacaatcct gatagattta atattaataa ttcctcagtt acttatatta atattgattc
     7081 caatgataca gactatatta ctattggtat cgataaccaa gaaccaataa aattacttgc
     7141 gagctataaa gacaaagaac tcgcccgtac tatcattaat gacaacatag ttgagaagac
     7201 tcatgatgat ataaataatg ctgaatttac tgatggtagt atcgttgtta gtggtaatga
     7261 gggtgctatt gactccaatg atacagccga tattactagt ggtatctata atcaagaacc
     7321 aataaaatca cctacgaacg atgaaaacaa aaaagtcggc caaaccatca tccatgaaaa
     7381 tgtagtagag aatactcatg atatcaataa caaggaaatg atcttcagcg cattaaaaga
     7441 aatatatgat ggagatcctg gttttatctt cgataagata tcacaaaaac tcagacatac
     7501 gataacggaa tttgatgaga atgggaaaag cgaaccaacg gacttattta cctggtacgg
     7561 taaagataaa aaaggcgatt ctctcgctat tgtaattaaa aataaaaacg gaaatgatta
     7621 cttatctctc ggttactatg atcaggacga ctaccacatt caaagaggaa ttcgtattaa
     7681 tggtgatagt ctcacccaat attgtagtga aaaagccagg aatgcttcag cgtggtttga
     7741 aagcagtaaa gctatcatgg cagaatcatt tgcaacaggt tccgatcatc aggttgtaaa
     7801 cgagctcaac ggggaaagac tgagagagcc aaacgaagtt tttaaacgtt taggtcgagc
     7861 aataagatat aattttcaag tggacgatgc aaaatttaga cgcgataatg taaaagaaat
     7921 aatttctact ttagtcgata acgaagttga ccgttctcaa aataaatatg accactttaa
     7981 ggaaatcgaa gataaagttg aaaaaaggct tcaaaatcgc caggctaaat atcaaaatga
     8041 aattaatcaa ctatctgcat tgggtgttaa ttttgatgat aattaaaatt gtccagtttt
     8101 tttgattttt caacgtctga atatcggaaa cagacaatga cgaacatcag tatttttaca
     8161 taattatgtc caggtcattg tcggtttatc ttcttttact tggggtaatt gagggataac
     8221 aacaaaaaag gtgagttttg tgactcacct tttttatttg tttcttattc agaatcgata
     8281 tcttttaaat catcctgaat cgcttgcgcg ttcggatttt cctgcggttt gagttcgccg
     8341 ccattagcga tgaaatcatg acgctggaag tacgcttcgc gcaccataat ataaggatcg
     8401 gacgactgac gcagcagacc atcggaatcc aacaactgcg cacgagtttc tatcccttca
     8461 agcgtccatt tacccacaga catcggccag gtcagccagg aaagaaccgg gtaaagacca
     8521 tccgccatat caccaccgtc atcacgcagc gtgaagctac cgtagaacgg taactgaacg
     8581 tatggcccat aacccacacc ataatgacca agcgtactac cgaagcggtg aggttcagtc
     8641 cgttgcagtt tcgggttcgc catccctgca acatcaataa agccgcccat ccccaaaatg
     8701 gtgttcagga aaaagcgggt aaagtggacc atcccctgat aagggtcgcc ctgcaagaag
     8761 tagttaacca tcaccgcagg ttcttcaagg ttgccagtaa agttgctcaa accgttacgc
     8821 gccggttgcg gaacataatc acgccaggcg acagcgaccg gtcgaacaat atacgggtct
     8881 aatacattga agttgaagtt gtacatggtg cggttgaacc cttctaacgg gtcagaacgc
     8941 ccttgctgat ctgtaccgga actcgcacac cccaccagaa gcgtagttcc cagagcaagc
     9001 gccgacaggc gaagcttcat aaatgtctcc ctgttttttt atggcttatg cagtttgcca
     9061 tccatgacgg aacgataccg tatccgcctg tttaggtgtg ggcgattgta acagcacgtc
     9121 aactgatgtc cagacgccct gatttgctga tttgatcata gcctggtaat cgccgccctg
     9181 taggctactt gattctatag aaacagaaaa aggcaaacgc ggccttttct acattttcag
     9241 agtaactccc gccagttgcg agcaaaaaag ccgctacgct ttagctatac gtgctaattc
     9301 aagagaagag accatggaca acgacaaaat tgatcaacac agcgacgaaa ttgaagttga
     9361 gagcgaagaa aaagagcgcg gcaaaaaaat agaaatagat gaagaccgac tcccctcccg
     9421 ggcgatggca attcatgagc atatccgcca ggatggtgaa aaagagctgg aacgcgacgc
     9481 aatggcacta ctgtggtcag ccattgcggc gggtctgtcg atgggcgctt cgctactggc
     9541 aaaagggata tttcatgtcg aactggaagg cgtgccgggc agcttcttgc tggagaatct
     9601 cggttatacc tttggtttta ttatcgtcat tatggcccgc cagcaattat ttaccgaaaa
     9661 caccgtgact gcggtactac ccgtcatgca aaaaccgaca atgagcaacg ttggcttact
     9721 tatgcggtta tggggcgtcg tgctgctggg taatattctc gggacaggta ttgcggcgtg
     9781 ggcatttgaa tatatgccaa tcttcaatga agaaactcgc gatgcatttg tcaaaatcgg
     9841 catggatgtg atgaagaaca cccccagcga gatgtttgcc aacgcgatca tttccggctg
     9901 gctgatcgcc actatggtat ggatgtttcc tgcagcgggt gcggcaaaga ttgtggtgat
     9961 tatattgatg acctggctta ttgccctggg tgacaccact cacatcgttg tcggttctgt
    10021 tgaaatcctc tatctggtgt ttaacggtac gctgcactgg agcgatttca tctggccctt
    10081 cgcactacct actttagcgg ggaacatctg cggcggcacc tttatcttcg cgttaatgag
    10141 tcatgcacag atccgcaacg acatgagcaa caagcgcaaa gcagaagcac gccaaaaagc
    10201 agaacgtgcg gaaaacatta agaaaaatga taaaaacccg gcataaatgg cgagggttta
    10261 agcaatcgag cggcagcgta cttaccccgc agtccattag cgggtatact catgccgcat
    10321 tgtcctctta gttaaatgga tataacgagc ccctcctaag ggctaattgc aggttcgatt
    10381 cctgcagggg acaccatccc gctgtacacc aacgtctacc attgtctata aatcccatgt
    10441 aaacatataa aaataaagag attttattct catgacgtcc atttccgtct attgaaatca
    10501 agtaaatcta tggggcataa ttaggggcat tgctggttcg attgtatatg tgcccccaac
    10561 atgtccagaa ataccctcaa caaattaaca gaccgtcagt gcaaagcagc taagccacga
    10621 gacaaaactt ataaattgtc tgatggtggt ggtctttatc ttgaagtatc ccagaccggg
    10681 tcgaagtatt ggcgaatgaa gtatcgccgc ccatcagata agaaagaaga cagacttgct
    10741 tttggtgtat atccaaccat tagcttacaa gacgccagag aaaaacgaga caacgccaaa
    10801 aagttgttag tcaaggggat agatcccaaa gcagagcaaa gagcagcaaa agcagaagaa
    10861 aaaggcgcat tcacttttga gacaatagga cgtcaatggg ttgaaagcca ccagatgtgg
    10921 aacgaagacc accgaaaacg cgtacttaga agccttgaaa tgtatatttt ccctcacatt
    10981 ggcacttcgg atattcgcaa actggaggcg atgacaattt tacctttgtt taagaaggta
    11041 gatgacgccg ggaaacacga tacagccaac cgacttaaac aacgagttaa ggacatcata
    11101 catagcgccc gtctgagcgg cattaagaca gaaatcatca cgaatgatct cgatgtccgg
    11161 ttaatgcaat atgaaactaa gcatcatgcc gcactacatc ccagagattt accagacttt
    11221 ttcactcgat tgggcagctt taaaggtaat cctcttaccc gccttgccat tgagctaacc
    11281 atgttgacat ttgttcgttc aagcgagtta aggtttgccc gttggaaaga gtttgacctt
    11341 gatcgggctg agtggattat ccctaaaaaa agagagccta tcgatggtgt gaggttttcc
    11401 acccgtggca caaaaacggg aaaagacgaa catatcgtgc ttcttagccg tcaggctgtt
    11461 tctattgtaa agcagttgag agaactaagc ggaagctacg atgttgtgtt tccgaatgaa
    11521 agaaacacca aaggcgtgat gagtgaaaac acggtaaaca aggcattacg cttaatgggc
    11581 tataacacgc aagaggacgt aactggacat ggattccgtg ccacagcctg tagttccctg
    11641 atggagtccg gtttgtggca ggaggacgca gtagagcgcc agatgagcca caaagaatat
    11701 aaagacgtta aaagagccta caagcacaag gctgattact tagaagagcg caaactaatg
    11761 cttcaatggt gggcagatta tctggacgcc aatcgagaaa tacatataag cccttacgag
    11821 ttcggaagga gaacacgccg tgactaagat gacaggacta gatgcgtttt ataacgagtc
    11881 agaagataaa atctggtttg gtaatctgat cagcgaactg gcaagactaa acaatgaaaa
    11941 tgaaagaatg atcgctgccg ctatcttaag taactacaga ttgagcaaag ctaaaccaga
    12001 tactttaggg gggctgggct tttatcagtt cgattgggta tcaggtttta caactaatga
    12061 cgactttgaa aagcagtgtg tggagttttt aggtcttctg gctatggggc atgaatacag
    12121 ggagagcccg ataccggggg aagaagggac tttcaacgtt aggaaagggg aagacttttt
    12181 atatcacgat gatgaggatg attggtatgg gtcttacgat tcgttttatt tcaagcgctc
    12241 cgaggttgtg aacttcttcc cagaactgga tatgaatgtt ccagataacg aaccagaaat
    12301 tggagcactg tcaaaaaaag tagaaagaga acaaaactgg agaggaaaag atacggcttt
    12361 aaagatgatc gccggaatgg cgattgcctt atttgaatca agcaaagatt tccatagtgg
    12421 aggaaacctt agtatcaata gggttagtgc ccaagtaata gaaaacaaca taatattcgg
    12481 gaacgatgaa gacagcgata cccacccaga tacatttcgg agattgctaa cgaacgcttt
    12541 ggataagtac gcccccaaat ttaagaaaaa agcaaccaaa aagcgtccaa aataaaggaa
    12601 ggatgttccc aaattgtgcg ttagaactaa aaaataacgc ccaatttggg gtaattctat
    12661 tcccaaaaat tttttctcca aattcacaga aaacttaaaa aaacaatgat ttactatctc
    12721 acatagtcaa gcaacgtatt acgaggtaga tatgaaaaag tcgctgattc ggctatctga
    12781 ggttttgaaa cgcactggct atagcaaggc gtgggtatat gctctgatga gccgaggaca
    12841 attcccccaa tcagtcaaga ttggtgctcg cgcaatagct tttattgaaa gcgagatcga
    12901 tgactggatc gatcaacgta tagccgaatc gcgtagtaac taaagaacag aaacaaacag
    12961 gatacaaaca atacgttatt aggtattaag aatgcagttg aaacctacca atcaaaagct
    13021 gaacgtaacc catgttgatt ttgaaacctt cgcggcactg attagatcag agccacaaaa
    13081 ggtgagcacc agcacctggg tttatgatgt gaaatccacc agcggtggaa aagctatcgg
    13141 cattgcctct ggcgatgatt acttagtaat agatctgatt taatcgcaca aaaattgtag
    13201 agtaatttgg ggagacagga agtctcctcc atattacatt ttggtacaac agtaagggaa
    13261 taaaatgatt aattacctga ccgctaaaga cgcagcagaa tatcttggcg tctgtctgtc
    13321 acgcttctat cagttaaagc gatatatccg aagctttcct ccctatataa atcagaccat
    13381 caatggacgc aagcgccggg tgtggcttgc ctccagcctt gatcaatttg ccgacagatt
    13441 tctgggggga cgaaaatgag cactcttaca cgctctcagg tcgccgcgaa tatacgcgat
    13501 atcctactaa gtggcaggaa gcttacccca aaagagtttg acgacatatt aaggaaagcc
    13561 ggaaatcacg aacgtagtcg cgttttaacg ctgttacgca acgattgggg aattcctgtt
    13621 gagcagttta aaacagaggc gtatcacgtc acagaacgca atttagaagc ataccacagc
    13681 gataaagacg aaacattgaa gatctggagg acgaacgcca gatatgtaaa gacattgcgt
    13741 aaggtaaaca taacgttgtc gttgttgcgt gggctggtgg gtaaagtgcc tgaagacacg
    13801 cttagaactg tttacaaggg tattgaaacc aaatacctgt gattgacaaa gcagaaagcc
    13861 gactttgcga gagtcggctt tgaagcaatt gaaatatatg aactttacac atggaattat
    13921 acgataaaga cgcgagtcta tccacaaaat cacaagccgt aatcaatcac atcaatggtc
    13981 gcacattagc ttatgtgaga tcagttacgc acaaacgtaa tgaattaaag gcggagaata
    14041 aacgccaatc atcaaaggct aaccactgca taccaccagc ttggacaaag aagaaatggc
    14101 gtacaatgag gcgagctttg tttactctgg atgtcgaatc aactagtggt atcccctact
    14161 cctttacctt cagacccgtt aagggaacaa cgccagagaa attcaacaaa gcgttaaagg
    14221 cacttaccag atggttgact tcgaaaggca atcagtgggt ttacgtcatg gagtgggctc
    14281 aacaccagtc agagccgcat gtacacgcaa taattatggt gaacgaaaga agcattaatg
    14341 ggcaagcagg caaaattgtc agcaaatggg taacactggc aaacaatgca atcccatcaa
    14401 atgttcctcc ccagtgggat ctagatttga aaggtcagca atattcagag cctacaaggt
    14461 gtataggtaa acagattgat tacatggcaa aacccgaaac taagttaaat gactttaata
    14521 gagaggcgag caagagccta tatgattggt cagatgccaa cgtttggggt tattcgaatg
    14581 gctgggcgcg gcatgagata aaaaaagaaa cactttctat cactggcttt catgctgtaa
    14641 gacgcatact aaaagccatt caggcatcaa ggaaagacaa ctacaaagtt aagcgacaga
    14701 tacagcgtac attgaaacga caagacataa atcgctcacc aatcaccccg ttccaagcac
    14761 cgttaattag caaacacgat tgggatttat cagtgatagc ggtcaccaaa gaggcagaga
    14821 cagagcgcag ggagttacta tcctcacaga caataaactc gaacaaagta caaacccacc
    14881 cgctgttaag gatgttaaga aaagcacgag tgagagcatc cagagccaga agagaaagcg
    14941 gaggttatga ccattactac tagtagtgac agatcgtgac aaggcagagc aaaaaacgac
    15001 caatataaga gaaatatccc tgcccaatcg ccaaaagcgc ggagcgcgga gcgcggcaag
    15061 cgccgcgaga ggcgcaagaa ggcgaaccta tccattgaaa cggtcagaag agcgcatcgc
    15121 gctcgatgac cgaagtcccc actaacaccg ttaacgaaag atcaaagtct tagaaaaaaa
    15181 tgacgtttct ttgaagggct tccatccgta aaaatcaaat tttgagataa agtacttata
    15241 gaaattaatc actctataag agtgagttga cgtgagtttg aagtgagcaa catattaaaa
    15301 atatatataa taatcaaaca tataataaca actactgagc aaaaatcacc agaaaacaac
    15361 gtcaacgtaa cgtcactctg taagcacttt ttcagtcact ttttgggttg aatacacgta
    15421 taaatcccca accatgaaat ttgagcgcgt aagcacgact ctgaaaggct ttttatcttt
    15481 taaccattta atatacctga ggtattgaag aagattctga gacatatact agaactttac
    15541 cgtgcacgac taacaacata aacgttatat ttatatataa atcaatgaga taaatacatc
    15601 cccatccaaa aaacaagcat aacgcaaaat tgcgttatca acttaaaagg tacgattgaa
    15661 tctgacagtc tgctctgagc gaaatgtggg cacgttcacc ataagtctcc agattgaaac
    15721 gaaatgatag tgttaaatgt tcgatagtta cttttctaag ctcaacgttc gtaatagtaa
    15781 tccgacacct cagtaaaatc tatttcctcc ctagaaagta atgattccat ctcatctagt
    15841 ttatcgattt gaggcaaaac acttgttggt gaaatcacaa cattaaagtc ctcattatat
    15901 cggtgaccta cccttgtgag ctctttacct aattgcaaca atatataatc acgtaaaata
    15961 agttgattcc gtctaaatcg aataagacga tagtaatgga agaaatcaga acattttgat
    16021 gaatcataat ttctaccatt ccacccggtt ttcctcgtag ctcggaataa tgcttgctct
    16081 tgcgtatttt tccaaatgct aaaatcaaag tgacttttcg ggttaggatt ttcgtatgtt
    16141 gcttgaggat aaaaattagt aacaccaaat tgatgcttgt caatgatctt taaagtccga
    16201 ttttgttcag acaacatccg ccttaaggac ctaggaattt cgaaacgcat tagtttgttt
    16261 ttatccaaga ttcttaattc tcttggaagc atctcatgat cccgttctaa atatctttca
    16321 caccgtttcg gcacatactg aaaatacact gaaaaaaaac aaaatatatt atcagaaacg
    16381 agaggagtaa tatgtatttt ttcttgctca ggaacgtttt gaacaaaata gtatgccgtt
    16441 ccttgccaaa tcaacgattg cgctactgcc tccaccaatt cacgtattgt ttcatctacc
    16501 gagtatttcc tatatctagc ttgtaaattt ccaaacattt tacgtatgag ttcttcgtcg
    16561 ttagtttcga cttgaaacaa attggcatca ccgtgattta caaaattaaa aactctagca
    16621 aaatcctcat aaaacatcct ttgatagaaa cctggtcgtt cattcttaga cttggtcaat
    16681 agtctatcaa caaaacttaa ttgctcagga tcgacgctat aaccgtaaag gggtatattc
    16741 ttgataattt tcattcaaaa tccttatcgt attccacgcc aaaacgacga gccaactcac
    16801 taacatggta atcgatcttg aagtcaattt gctcgagttg cgggaaaatt tcgagagcac
    16861 aaaagagatg cgatgcatct gaatgggagt ttaagaactc tctgacaact ttgttcgtat
    16921 gagatttccc tcgattcatt cccatttgat cgctaaagcc atcatgtctt gcgcggcgct
    16981 caaattccat tttccagtta agacgaatca tatctctcgg tgatgcgcac aagctgaaat
    17041 tgcgcggatt tgaaagaatc atcattctct tcaaatactg ctgtttgttg ggaacagact
    17101 cgagccaaga cttaaaccta tcaagtactg ttttagatag ttttgcatta tcgaaataag
    17161 cactgtaaag gatagcaaaa cctagaactg acatcagatc gttgatcgcg gatgaaagga
    17221 gatgcagtcg aaactcgttg tttatatcga gtttcggatc tacaaatttg gtatctgatg
    17281 ccagcaatga gagaaccatg aacataggga ataccttgct cagtttattt tcatcattct
    17341 gttcaagcgc atgtatacat tcctcagcca actcaaaata tatctgtcca aaatgatccg
    17401 gtagttcatc atcttgctct tgttcaatga tatgtcccac aatttccacc ctaccaagaa
    17461 tagctatagc ttcatcctgt gtaacgacaa actgtttgag catttcagca atattaattt
    17521 ctggtaatgc gtatagcttt tcagagtagt gctcgtactt tctgtatctc tccaagagca
    17581 gagaaagttc attaaaccaa tggggaaact tcaaatgatt atgtaggcta gcgagaacga
    17641 cttgcgttgc ggcttccggc atcttcatgt ttatcagtga gttaacgaaa tctggaacca
    17701 tgtttacgtg aaaatcgcat acttctggca gaattttaga atattgttgt aataacttct
    17761 gaaccgccaa ctgctggacg tactttggtt tggataatct ctgtccttca atctcttttt
    17821 cgaattcgat acgttctacg ataaaggcca actcaacctg cagaaaagca ggcaaagtgt
    17881 gtagagaggt ctctgtccag atatcatctt taaagaactg ctccaattct ttctcaaaag
    17941 taatcatgcg gcgtaacatt tcaaggcaaa gattgctgcc taatgcagcc catgcatccg
    18001 caattccaac ttttatcacc ttttcctcaa aataattaac gacatcagac ggtgtaaagg
    18061 ctttttcaat gatcgtcttc attttccgaa gttcacgtat accaatatcg aactgaaact
    18121 gttgtgcata agttaatata cgagatgaga atagactgat gagatccaac ccgagcttga
    18181 aatcaccggc tttgaatgca agttcaatat ggcctaccaa cctattgata atttcattct
    18241 ctaaccaaag ataatttggt tcttcttcaa ccgttaattg gctccttgtt tttaaagcaa
    18301 tggatgttac actgtcgcca gctaagaacc attgcttatg ttttattcgt ctagggaacc
    18361 agtaactttg ataatcaatt ctgtgtttct gattaaggta atgaagaagt aatgccgaat
    18421 aaacattact gagagcaggt aaattatctc cgagtcctac tttttcacat atcaggcgat
    18481 catcaatgta gtttaactgc tcaaaggcca aatgtgcggc tttagagtga tgattagcta
    18541 aagatactga attcttggga ttagacgcac cttcgatgtg tcgtgcaatt cgtgggagta
    18601 tttcacttcg ggctaactga tttaggtcaa aaaaattgaa taatctttga cctaatggga
    18661 acagagctaa ggaactaaca agtgtcagca atgttcccat tgcataaacg aaaagaccgg
    18721 gttcaacccc gaacaagtgg agagcagttg cactaagaca aaacaccgca gaaagcacta
    18781 aagtccgcga atacacatta cctacctgct cggtagtaag catttgtata atatctcgcc
    18841 gaagtctggt atatcctgta cttagaatga tacctattgt agcaaagtat attgaaaata
    18901 tagcggtaag tatctgtgcg tacaacctaa gttgatcaat attaaatttt ttttcatcat
    18961 agctaagagg atgaagcaaa atcgtattat tccgaatata atcttcaaca aaacccagtg
    19021 caaataacgt taatatgacc cataacaaat tttttaaacc taccaacgca aaagacataa
    19081 aaacatgagc gttagactta gctttaaaaa cagacgtacg atgtatgtat gacgcacgtt
    19141 ctaaaataaa tctaaccctc acaagatctg ttcttaattt ccagaaccac ttttgagaaa
    19201 taagccaaac cttcattata tcaatcaagt gatacctgcc ttctcatctc tacatgcaac
    19261 ataaaaatgc ttactatggt ggcaaacaaa atcaagaatc atatgtttga acacttttta
    19321 cgcacgaatt atccggtgta agatacttat agtcaaatgg cagggctaat ctcatctgta
    19381 ttgagctatt aacagccata tgtaagacta agaaacgcgc ccagcaaagg cgcgttctgc
    19441 atcaggtaag tttgctgtgg tctacggttt ccatccaatc tggagcaatt cccaaacgtt
    19501 ccgctatctc ttcgttggtc atgtcttcag ttgagaaatt agccagagaa tcgttaactt
    19561 caggatagtc aggggagagt aacaggtatt cccgcaacgc tttcagatct ttttccattt
    19621 taacgctcct tgtttattga cagtcgaacg atggtgaaga ttcttgccat ctcaagtaca
    19681 ggcaagaaaa tccaccagaa caccaaatta tctcataagg ttaaatcggt gtcgggctat
    19741 ttctttcggc taggtgagtg taccgcctta accaccggca attatcattg tctcaattgg
    19801 gtataccgta atgacaaggt gatacaaagt ctcaataggg ttgtatagga acattttgac
    19861 tacacaaaat gcattatgta tcatttgcac ttatattcac aaccctattg agacaatgca
    19921 atgaacgtta gaatttactg ccgagcatca actgaggggc aacacgctga tcgagcactt
    19981 accagcctac gtgaattttc aaaatcaaaa ggctggcaga ttgcaggaga gtatatagaa
    20041 aatgctagcg gggcgaagct tgagcgcgta gagcttatgc ggctattgtc tgaagcccag
    20101 tccggcgatc tgttgctagt ggaagctata gatcgtctga gtcgcctaga acatagcgca
    20161 tgggtcgaac tgaaagatac gctcaaccga aaagggttaa tcatcgtatc aatggattta
    20221 ccaacgagct ggcagatggt cgagatggca ggcaatgacc taaccagcgg catcctgaga
    20281 gccgtaaatg ccatgttgat tgatatcctt gccacaatgg cacggcagga ttacgaaaca
    20341 cgtcgtaaac gccaacagca aggcattgag cgagctaaaa gcgaaggaat atacattggc
    20401 agggagaaaa atcaggaagc ccgagagatc gttagagaga tgttggagca aggagtaaaa
    20461 cctgaactca tcatgaaggc agcaggaatc agccgagcga cttactaccg tataaaaagt
    20521 gagctgtcag tcgtaaaatc agaataacct ttgaaaggtc gtcaggcttg tttcatctgc
    20581 taaaagccag catttctgac cataatacct gggggcacct actggggcac ccaccaaaat
    20641 cgaaagacaa ataagttata attcaatctg ttagtttact tttcgattcc tgcaggggac
    20701 accagatacc cttcaaacca aatctacctt caccccgtaa aagatgggtt tggcgcacac
    20761 tagccctata tctactcatt tttactgcaa caggttgaaa tctcagcact gtcagaaagc
    20821 gctgatgact aaacagccct gggccgggcg atgtaaccat catacagaat cctgatagcg
    20881 aaatatggcg tgactcgata cttcactctg caatgcattc cttgatgaat tcgcaggccc
    20941 gtgatactcg ggacaggtcg ctgaatgacg acaatgtcct ggaaatcagc gaaccgcgta
    21001 tccggagtac atttgagcga ctgtaccaga acatgaatga ggcgtttgga ttaggtgatt
    21061 attagttggg ctaagcattt ttgtattatt attttccggt tgagggatat ggagatatcg
    21121 acaacaaccg gaaaaagttt acgtctatat tgctgaaggt acaggcgttt ccataactat
    21181 ttgctcgcgt tttttactca ggaagaaaat gccaaatagc aacatcaggc agacaatacc
    21241 cgaaattgcg aagaaaactg tctggtagcc tgcgtggtca aagagtatcc cagtcggcgt
    21301 tgaaagcagc acaatcccaa gcgaactggc aatttgaaaa ccaatcagaa agatcgtcga
    21361 cgacaggcgc ttatcaaagt ttgccacgct gtatttgaag acggatatga cacaaagtgg
    21421 aacctcaatg gcatgtaaca gcttcactaa tgaaataatc caggggttaa cgaacaacgc
    21481 gcaggaaagg atacgcaacg ccataatcac aacaccgata agtaatgcat tttttggccc
    21541 tacccgattc acaaagaaag gaataatcgc catgcacagc gcttcgagta ccacctggaa
    21601 tgagttgaga taaccataca ggcgcgttcc tacatcgtgt gattcgaata aacctgcata
    21661 aaagacagga aagagttgtt gatcaaaaat gttatagaaa gaccacgtcc ccacaataaa
    21721 tatgacgaaa acccagaagt ttcgatcctt gaaaactgcg ataaaatcct ctttttttac
    21781 ccctcccgca tccgccgcta tgcactggtg atccttatct ttaaaacgca tgttgatcat
    21841 cataaataca gcgccaaata gcgagaccaa ccagaagttg atatggggac tgatactaaa
    21901 aaatataccg gcaaagaacg cgccaatagc atagccaaaa gatccccagg cgcgcgctgt
    21961 tccatattcg aaatgaaaat ttcgcgccat tttttcggtg aagctgtcaa gcaaaccgca
    22021 tcccgccaga taccccaggc caaaaaagag cgcccccaga attagaccta cagaaaaatt
    22081 gctttgcagt aacggttcat aaacgtaaat cataaacggt ccggtcaaga ccagaatgaa
    22141 actcatacac cagatgagcg gtttcttcag accgagttta tcctgaacga tgccgtagaa
    22201 catcataaat agaatgctgg taaactggtt gaccgaataa agtgtaccta attccgtccc
    22261 tgttaatcct agatgtcctt tcagccaaat agcgtataac gaccaccaca gcgaccagga
    22321 aataaaaaag agaaatgagt aactggatgc aaaacgatag tacgcatttc tgaatggaat
    22381 attcagtgcc ataattacct gcctgtcgtt aaaaaattca tgtcctattt agagataaga
    22441 gcggcctcgc cgtttacttc tcaccttcca gttcttgtcg acatggcagc gctgtcattg
    22501 cccctttcgc tgttactgca agcgctccgc aacgttgagc gagattgata attcgtcgca
    22561 tttctctctc atctgtagat aatcccgtag aggacagacc tgtgagtaac ccggcaacga
    22621 acgcatctcc cgccccagtg ctatcgacac aattcacaga cattccagca aaatggtgaa
    22681 cttgtcctcg ataacagacc accacccctt ctgcaccttt agtcaccaac agcatggcga
    22741 tctcatactc ttttgccagc gcgcatatat cccgatcgtt ctgtgttttt ccactgataa
    22801 gtcgccattc ttcttccgag agcttgacga catccgccag ttgtagcgcc tgccgcaaac
    22861 acaagcggaa caaatgctcg tcttgccata gatcttcacg aatattggga tcgaagctga
    22921 caaaacctcc ggcatgccgg atcgccgtca tcgcagtaaa tgcgctggta cgcgaaggct
    22981 cggcagacaa cgcaattgaa cagagatgta accattcgcc atgtcgccag cagggcaagt
    23041 ctgtcgtctc taaaaaaaga tcggcactgg ggcggaccat aaacgtaaat gaacgttctc
    23101 cttgatcgtt cagatcgaca agcaccgtgg atgtccggtg ccattcatct tgcttcagat
    23161 acgtgatatc gacaccctca gttagcagcg ttctttgcat taacgcacca aaaggatcat
    23221 caccgacccg acctataaac ccacttgttc cgcctaatct ggcgattccc accgcaacgt
    23281 tagctggcgc gccgccagga caaggcagta gccgcccgtc tgattctggc aagagatcta
    23341 cgaccgcatc ccctaaaacc catactttgg ctgacatttt tttcccttaa attcatctga
    23401 cttacgcata gtgataaacc tctttttcgc aaaatcgtca tggatttact aaaacatgca
    23461 tattcgatca caaaacgtca tagttaacgt taacatttgt gatattcatc gcatttatga
    23521 aagtaatgga ctttattttt ataaaagtta acgttaacaa ttcaccaaat ttgcttaacc
    23581 aggatgataa aaatgacgca atctcgatta catgcggcgc aaaacgcact agcaaaactt
    23641 cacgagcgcc gaggtaacac tttctatccc cattttcacc tcgcgcctcc tgccgggtgg
    23701 atgaacgatc caaacggcct gatctggttt aacgatcgtt atcacgcgtt ttatcaacat
    23761 cacccgatga gcgaacactg ggggccaatg cactggggac atgccaccag cgacgatatg
    23821 atccactggc agcataagcc tattgcgcta gcgccaggag acgagaatga caaagacggg
    23881 tgtttttcag gtagtgctgt cgatgacaat ggtgtcctct cacttatcta caccggacac
    23941 gtctggctcg atggtgcagg taatgacgat gcaattcgcg aagtacaatg tctggctacc
    24001 agtcgggatg gtattcattt cgagaaacag ggtgtgatcc tcactccacc agaaggaatc
    24061 atgcacttcc gcgatcctaa agtgtggcgt gaagccgaca catggtggat ggttgtcggg
    24121 gcgaaagatc caggcaacac ggggcagatc ctgctttatc gcggcagttc attgcgtgaa
    24181 tggaccttcg atcgcgtact ggcccacgct gatgcgggtg aaagctatat gtgggaatgt
    24241 ccggactttt tcagccttgg cgatcagcat tatctgatgt tttccccgca gggaatgaat
    24301 gccgagggat acagttaccg aaatcgcttt caaagtggcg taatacccgg aatgtggtcg
    24361 ccaggacgac tttttgcaca atccgggcat tttactgaac ttgataacgg gcatgacttt
    24421 tatgcaccac aaagcttttt agcgaaggat ggtcggcgta ttgttatcgg ctggatggat
    24481 atgtgggaat cgccaatgcc ctcaaaacgt gaaggctggg caggctgcat gacgctggcg
    24541 cgcgagctat cagagagcaa tggcaaactt ctacaacgcc cggtacacga agctgagtcg
    24601 ttacgccagc agcatcaatc tatctctccc cgcacaatca gcaataaata tgttttgcag
    24661 gaaaacgcgc aagcagttga gattcagttg cagtgggcgc tgaagaacag tgatgccgaa
    24721 cattacggat tacagctcgg cactggaatg cggctgtata ttgataacca atctgagcga
    24781 cttgttttgt ggcggtatta cccacacgag aatttagatg gctaccgtag tattcccctc
    24841 ccgcagggtg acatgctcgc cctaaggata tttatcgata catcatccgt ggaagtattt
    24901 attaacgacg gggaggcggt gatgagtagc cgaatatatc cgcagccaga agaacgggaa
    24961 ctgtcgctct atgcctccca cggagtggct gtgctgcaac atggagcact ctggcaactg
    25021 ggttaacata atatcaggtg gaacaacgga tcaacagcgg gcaagggatc cgcgtcactc
    25081 ttcccccttc acgatcttca ataatatgca atgcagcttc ccgcccgata atgtcatgtg
    25141 gaagctgaat tgtggtcagc ggcggtaaaa acagatgccc gacgccaacc agattatcaa
    25201 agcccattac ggcgacatcc tgcgggatac gtaccccctt cgccaggaga acctgataag
    25261 cgacaaaggc tgcgcgatcg ttaccacata tcagaacatc aaaatctggt ttgccagatt
    25321 tgaagtgggc attgagtaaa cttgcgagat cggtgtagtg atcatcacct gttgccatgt
    25381 gaaattgttt cacctcagcc agatctcgtc cagcatcacg ccaggcctgc tcaaatccct
    25441 gccgacgata ccctgttgcc aacgcacttt ccggtagcca gaagcataac ggttgacgat
    25501 agcccgccgc gagcaaatgc tgtgttgatt catattgtgc agtgtaatca tcagggatat
    25561 aactgggtaa cgctgggtca tccgccacac agttcgccaa tacaatattt tcaccataca
    25621 gagactcagg cagcgtgata tgtcgcagcc ccattgtagt atagataatg ccatccggac
    25681 ggtgggcaag cagctgacga gcagcacggg cagcgtcatc ttcagaaaaa atattgatta
    25741 aaaaactatt ccagccgaac tcgctggcgg tttgctcaat ggcaagcaga atatcaacag
    25801 agaaaggagt ggtagcagtg tcctgcgcca gcacggcgag agtcgacggc ttacgtcctt
    25861 gagcgcgcat cttacgggcg gaaagatcag gaacataatt cagggtctgg attgcctgca
    25921 atacgcggtc acgcgttgca ggacgcacag attctgcatt atgcatcacc cgggagactg
    25981 tcatcatcga cactcccgcc aggcgtgcga catcctttaa tgaagccata cccaagccgt
    26041 ttgccgtaaa acgggcactg tagcagaaac agacgtcact ggcgagatcc aacgatattc
    26101 tcgacgccct atcacccgac acggcaatac aataaaaaat aacaataatt cccggacaat
    26161 tgtacccaat tccgcctctg ttctcgccaa cgagtaacca tgtaggcaag taggtaaaac
    26221 atgccaactg gcatcgccaa cctggagaag aaaatataaa tatggatcat tgtgctgccg
    26281 ctgtatcccg acatcagccc ggaaattatt gcgattgcta tcggttcagg tgcgattggc
    26341 tgcacgatcg ttacggactc gcttttctgg ctggtgaagc aatattgcgg cgctacgctc
    26401 aatgaaacat ttaaatacta tacgaccgcg acatttatcg cttcagtcgt cgctctggcg
    26461 ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag acgtactatg gaaaacgcta
    26521 aaatgaactc gctcatcgcc cagtatccgt tggtaaagga tctggttgct cttcaagaaa
    26581 ccacctggtt taatcctggc acgacctcat tggctgaagg tttaccttat gttggcctga
    26641 ccgaacagga tgttcaggac gcccatgcgc gcttatcccg ttttgcagcc tatctggcaa
    26701 aagcatttcc tgaaactgct gctactgggg ggattattga atcagaactg gttgccattc
    26761 cggctatgca aaaacggctg gaaaaggaat atcaacaacc gatcagcggg caactgctac
    26821 tgaaaaaaga tagccatttg cccatttccg gctccataaa agcacgcggc gggatttatg
    26881 aagtcctggc acatgcagaa aaactggctc tggaagcggg gttgctgacg cttgaagatg
    26941 actacagcaa actgctttct ccggagttta aacagttctt tagccaatac agcattgctg
    27001 tgggctcaac cggaaatctg gggttatcaa tcggcattat gagcgcccgc attggcttta
    27061 aggtgacagt acatatgtct gctgatgccc gggcatggaa aaaagcgaaa ctgcgcagcc
    27121 atggcgttac ggtcgtggaa tatgagcaag attatggtgt tgcagtcgag gaaggacgta
    27181 aagcagcgca gtctgacccg aactgtttct ttattgatga cgagaattcc cgcacgttgt
    27241 tccttgggta ttccgtcgct ggccagcgtc ttaaagcgca atttgccgag caaagtcgta
    27301 ttgtcgatgc tgataaccct ctgtttgtct atctgccgtg tggtgttggc ggtggtcctg
    27361 gtggcgtcgc attcgggctt aaactggcat ttggcgatca tgttcactgc ttttttgccg
    27421 aaccaacgca ctccccttgt atgctgttag gcgtccatac aggattacac gatcagattt
    27481 ctgttcagga tattggtatc gacaacctta ccgcagcgga tggccttgca gttggtcgcg
    27541 catcaggctt tgtcgggcgg gcaatggagc gtctactgga tggcttctat acccttagcg
    27601 atcaaaccat gtatgacatg cttggctggc tggcgcagga agaaggcatt cgtcttgaac
    27661 cttcggcact ggcgggtatg gccgggcctc agcgcgtgtg cgcatcagta agttaccaac
    27721 agatgcacgg tttcagcgca gaacaactgc gtaatgccac tcatctggtg tgggcgacgg
    27781 gaggtggaat ggtgccggaa gaagagatgg agcaatatct ggcaaaaggc cgctaattac
    27841 ttttcaattc agcatcgcca tcctttccct gggtgagcga tgctgccgat ggcgcagact
    27901 taaggtccct ggtcttacct gctataaccc cctcctttta tcatcaccca acgcctttcg
    27961 ctgtaaacgg cggtttcgca aaccaaacca aaactgtaag taagataaaa gcaatagccg
    28021 ccatacggaa aatctcattt gcagaaattg aaagtgactg ctgagttatt tcattattaa
    28081 tttcattaag aacccctgaa agcgaaccat agtatttatc cataatttgt gacgatgaat
    28141 taaacacggg gttaaattga tcgatggttg ctgtcaactg actatggtgt aacgattcgc
    28201 gtcgtcccca cagcgtcatt gtcaacgacg taccaactga tcctgacaag gtgcgaaaaa
    28261 aattactcat actcgaggca ttggcaaatt tattatctgg caaccctgaa aacgaaatcg
    28321 ttgttaaggg taaaaagaaa caggcaacgg cgaatccctg gaaaaactgt ggcaaaatga
    28381 tgcctgtaaa atcaatcgtt ggcataaatg tcacagaacg ccagtaatag caaaccgcat
    28441 acattaaaaa actaaatgtc accaacaacc gcatatcaat tttgttgcca taacgtccta
    28501 tcaaaggtga tattaatagc ggcatgatgc cgatgggcgc ataggcaagt ccggcccata
    28561 tcgcattata ccccatcgtt tcctggagta actgcggcat aaggactatc gccccagagt
    28621 aaaataaata cgcgcatgtg atactcacaa taccaatggt gaaattacgg gacttaaaca
    28681 aactgagatc aagaatcggg ttctctgagg tcgactccca aatgactaaa gaaatcagag
    28741 aaataactga cactactgtt agtattatta ttgtactcga gttgaaccaa tccaggtcgc
    28801 gccctttatc aagcattatc tgcaagccac caacaccgag cactaacagg gtcagtcctg
    28861 gtagattcat tttgaccggt gaagtctcag tttctcttcc tttaagtaag gttaagcata
    28921 atgtcagaac gataatcccc atagggacat tgattaaaaa tatccaaccc cagctaaagt
    28981 tatcacaaat ataaccgccc aatatcagcc cacatatcgg cgcgataatc acggtcatag
    29041 accataatgc cagagcaaat gttctttttt ctggtggata attccttaat aataaactct
    29101 gtgacagtgg aattaacggc cccgccatta acccctgaac gactctaaaa aatatcagca
    29161 catcaagatt ggtcgatagg ctacacatta atgaagatag cgaaaaaaag gttactgaaa
    29221 gtaaaaataa tcttaattcg cctattcttt gtgccaacct gccggtaaca gggatcgcaa
    29281 tggcatttgc tacaccaaac gaagtgataa cccaggtgcc ttcgtctgtt gatgctccca
    29341 gaaagccaga tattgtcggt attgcgacgt tagaaatagt ggaatccaac atttgcataa
    29401 acgtcgctaa tgacaatgca atagtgacgc accataacgt cccaccggtt aatggtgccg
    29461 gagttgattt agtgattgcc attaaagttg tccattatgc gaaataatgt tgctaatttc
    29521 tttttctatc ggactggtat cgataactaa agccttactg gtataagcag gcatggacgt
    29581 cacggttgaa gccagctcag gcatctcggc aatgtcttcg ttcttcgtat caatagttgc
    29641 tgtcatcgat aaaccaatac gtaagggatg ttccatgagt tcttttggat caagagaaac
    29701 ttcaaccggt acacgctgaa cgattttgat ccagttccct gtcgcatttt gtgcaggtaa
    29761 taaggagaac gcattgccgg ttcccatatt gatccctgtc acccgaccat gaaacacaac
    29821 attttcacca taaagatcgc tgataatatt gaccgattga ccaatccgta catccgtgag
    29881 ttgtgtttct ttaaagttgg cattaaccca catttgacgt gccggtacga cagccattaa
    29941 cgattgtccg ggactcactg tttcgccgac ctgaacgctt ctctgggcaa tatagccggt
    30001 aacaggactc ttaatatccg tacgtttaag cgctaaccag gcttctttgg ttgcatccgc
    30061 cgcttcaacg acttgtggct gacggtttaa tggtgtgttc attactaaag ctttattcgc
    30121 tttataagcc tggatagcgg cattcaatgc cgctttgcta cttattaacg tatctttggt
    30181 atgctccagc gtttcttttg aaataacccc ctgcttcgct aaaggtacac gacggttata
    30241 atcttctaaa gattgttgat actgaatacg tgctgaagcg acttcggcac tgtattgttt
    30301 atcctgtaag tacagtttat tcgtttgccg aacaatattg gccagattat ttttagcttt
    30361 attgagtgcg atagtggcat cagtcttatc cagtgagact aaaatgtcac cctgtcgaac
    30421 gtagttcgta tctttatgat taacgacagt gacgctgcct gaaacttgtg cagaaattgg
    30481 atctgcattc cccgtgacat aggcatcatc tgtactaatc atgtcttcta attccattga
    30541 ccaataggca taggcacctg aaaacgcaat aaataaaact accgccaata aagaaaagta
    30601 ttttcttctg ttagaatgtt ttttatttga attaatctgt tccactatta tctctcattt
    30661 cgcatagatg aaatttatga attgaatgaa aagagtattc attcttcata tcaaacacag
    30721 tgcatgtgtt gcacattgtc ccaggaacgt tggaggccta atttaaatga ggtgcaaaaa
    30781 aaacaacaga ggactaaacc gtggcttttg caatacaatt cttacgcatg taggattagt
    30841 gagaagactt atagtactaa tgtgaaacta taaatcatcg gtacaatccc tgattttatt
    30901 gttgacattt catatatgcc gactatttat atggtatact tgtcgaatta tcttaaagga
    30961 agctcaaatt ttcttatttt tattgagaaa atgagatgat gccttatgtc tgtattacta
    31021 cagggagaag ggagatgctt cattgcaaag ggaataatct atgaacgcaa taattattga
    31081 tgaccatcct cttgctatcg cagcaattcg taatttattg atcaaaaacg atattgaaat
    31141 cttagcggag ttgactgaag ggggaagtgc cgttcagcgg gtggaaacac ttaaacctga
    31201 tatcgtcatc attgatgtcg atatccccgg agttaacggt atccaggtgt tagaaacgct
    31261 gaggaagcgc caatatagcg gaattattat tatcgtctcc gctaaaaatg accattttta
    31321 cgggaaacat tgtgctgatg ctggcgctaa tggattcgtg agtaaaaaag aaggcatgaa
    31381 caatatcatt gcggctattg aagctgcaaa aaatggctac tgctatttcc ccttctctct
    31441 caaccggttt gttggaagtt taacgtccga ccagcaaaaa ctcgactcct tatcgaaaca
    31501 agaaattagt gtcatgcgat atattcttga tggcaaggat aataatgaca ttgctgaaaa
    31561 aatgttcatc agcaacaaaa ctgtcagcac ttataaaagt cgcctgatgg aaaaattaga
    31621 atgtaaatca ctgatggatc tttacacatt cgcacaacgt aacaaaatcg gctaaccaca
    31681 tgaagttttt accctatatt tttcttctct gttgtggtct ttggtcgacc ataagtttcg
    31741 cagacgaaga ttacatcgaa tatcgtggca tcagtagtaa caaccgtgtc acgcttgatc
    31801 cactacgtct gagcaacaag gaattacgtt ggttagcgag caaaaaaaat cttgtgattg
    31861 cagtacacaa gtcccaaacg gctactttgt tgcataccga ttcgcagcaa cgggttcgtg
    31921 gtattaatgc tgattattta aatcttttaa aaagagcgtt aaatattaaa ttaacactcc
    31981 gggaatacgc agatcatcaa aaagcaatgg acgcgcttac agaaggggaa gtcgatatag
    32041 tgttatcaca tttagttgct tcgccgcctc ttaatgatga cattgctgca accaaaccgc
    32101 tgataattac ctttccggcg ctggtcacca cccttcacga ttcaatgagc ccgcttacct
    32161 catcaaaacc agtaaatatt gctcgggtag caaattaccc tccagatgag gtaattcatc
    32221 aatcatttcc aaaagcaaca attatctctt ttacaaattt atatcaggca ttagcatccg
    32281 tctcagccgg acagaatgat tactttattg gtagtaacat tattaccagc agtatgattt
    32341 cccgctattt cactcactcc ttaaatgtag tgaaatatta taactcgccg cgtcaatata
    32401 actttttctt aaccagaaaa gattcagtcg ttcttaatga agtactcaat cgatttgttg
    32461 atgctttaac aaatgaagtt cgctatgaag tatcacaaaa ttggcttgat acaggaaacc
    32521 tggcctttct gaacaaacca ttagaactca ctgaacatga aaaacagtgg attaagcagc
    32581 atcccgattt aaaggtgctg gaaaatcctt actcgccacc ctattctatg acagatgaaa
    32641 ctggctcagt tcggggcgtg atgggtgata ttcttaatat tattaccttg caaacaggtt
    32701 taaatttttc tccgatcacc gtttcacaca atattcatgc ggggacacag cttaaccccg
    32761 gtggatggga tatattgccc gccgctattt atagtgaaga tagagaaaat aatgttttat
    32821 ttgctgaagc cttcataaca acgccttacg tttttgtcat gcaaaaagcg cctgacagtg
    32881 aacaaacatt aaaaaaagga atgaaagttg ccattcccta ttattatgag cttcattcgc
    32941 aattaaaaga gatgtatccg gaggtagagt ggataaaagt cgataacgcc agcgctgcat
    33001 ttcacaaggt caaggaaggc gaacttgatg ctctggtcgc gacacagtta aattcacgtt
    33061 acatgatcga ccattactat cctaatgaac tttatcattt tcttattccc ggcgttccga
    33121 atgcatcgct ttcgttcgct tttcctcgcg gagaaccgga acttaaggat attattaata
    33181 aagcactgaa tgcaattccc ccaagcgaag ttctgcgcct gacggaaaaa tggattaaaa
    33241 tgcccaatgt gaccattgac acatgggacc tctatagcga gcaattttat attgtcacga
    33301 cattatccgt tttattagtt ggcagtagcc ttttatgggg attctacctg ttacgctcag
    33361 ttcgtcgtcg taaagtcatt cagggtgatt tagaaaacca aatatcattc cgaaaagcac
    33421 tctcggattc cttaccgaat ccaacttatg ttgtaaactg gcaaggtaat gtcattagtc
    33481 ataatagtgc ttttgaacat tatttcactg cggattacta caaaaatgca atgttaccat
    33541 tagaaaacag tgactcaccc tttaaagatg ttttttctaa tgcgcatgaa gttacagcag
    33601 aaacgaaaga aaatcgaaca atatacacac aggtatttga aattgataat ggcatcgaga
    33661 aaagatgcat taatcactgg catacattat gcaatctgcc agccagcgat catgctgttt
    33721 atatttgtgg ttggcaagat attaccgaga cgcgtgattt aattcatgca ctcgaggtag
    33781 agagaaataa agcgatcaat gcaactgtcg caaaaagcca gtttctggca acaatgagtc
    33841 acgaaataag aacaccaata agctccatta tgggcttcct ggaacttctg tcgggttctg
    33901 gtcttagcaa ggagcaacgg gtggaggcga tttcacttgc ctacgccacc ggacaatcac
    33961 tcctcggctt aattggtgaa atccttgatg tcgacaaaat tgaatcgggt aactatcaac
    34021 ttcaaccaca atgggtcgat atccctactt tagtccagaa cacttgtcac tctttcggtg
    34081 cgattgctgc aagcaaatcg atcgcattaa gttgcagcag tacgtttcct gaacattacc
    34141 tggttaagat cgaccctcag gcgtttaagc aggtcttatc taatttactg agcaatgctc
    34201 tcaaatttac caccgagggg gcagtaaaaa ttacgacctc cctgggtgac attgatgaca
    34261 accacgctgt tatcaaaatg acgattatgg attctggaag tggattatcg caggaagaac
    34321 aacaacaact gtttaaacgc tatagccaaa caagtgcagg tcgtcagcaa acaggttctg
    34381 gtttaggctt aatgatctgc aaagaattaa ttaaaaacat gctgggtgat ttgtcattag
    34441 aaagtcatcc cggcatagga acgacattta cgatcacaat cccggtagaa attacccaac
    34501 aagtggcgac tgtcgaggca aaagcagaac aacctatgac actccctgaa aagttgagta
    34561 tattaatcgc ggatgatcat ccaaccaaca ggctattact caaacgccag ctaaacctat
    34621 taggatatga tgttgatgaa gccactgatg gtgtgcaagc gctacacaaa gtcagtatgc
    34681 aacattatga tctgcttatt actgacgtta atatgccgaa tatggatggt tttgagttga
    34741 ctcgcaaact ccgtgagcaa aattcttcct tacccatctg ggggcttaca gccaacgcac
    34801 aggctaacga acgtgaaaaa gggttaaatt gcggcatgaa cttatgtttg ttcaaaccgt
    34861 tgaccctgaa tgtactgaaa acacatttaa gtcagttaca ccaggttgcg catattgcac
    34921 ctcagtatcg ccaccttgat atcgaggccc tgaaaaataa tacggcgaaa gatctacaac
    34981 tgatgcagga gattctcatg actttccagc atgaaacgca taaagatcta cccgctgcgt
    35041 ttcatgcgtt agaagctggc gataacagaa ctttccatca gtgtattcat cgcatccacg
    35101 gtgcggctaa catcctgaat ttgcaaaagt tgattaatct tagccatcag ttagaaataa
    35161 cgcccgtttc agatgacagc aagcctgaaa ttcttaagtt gctgaactct gttaaagaac
    35221 acattgcaga gctggaccag gagatagctg ttttctgtca acaaaatgac taaatagcgg
    35281 ctcccacaat attcaaatgt gggagctatt taccagcaca tcttacattt atgatgagaa
    35341 ctcctggcga atttgttccc catgctggtc gagcgttgcc gctcccggca taacatgcgg
    35401 gtccgcgcag ccgctgattt ttatcggatt acctggcatc attattcccc cggcttcaat
    35461 caacatattt ctcgcctgag tttgtggcaa attaatggcc tcagccacac ttaataacgg
    35521 cgcgacgggc acaccaactt catgtatttt ggctaaccaa acttcagctg cctgcgtttt
    35581 taacgttcgc tcaatatatt gtttaagaat agcctggttt tgtacgcgta aaatattgct
    35641 gctaaatcgg ggatcattaa ccagttccgt aagctccagt gcctggcata acgcagaaaa
    35701 aagcttgtca ttaccacaac aaatagtaat cggcttatcc tgagtatcaa aaacatcaaa
    35761 aggtgccatg tagggatggc gatttcccag gcgttgtggt gacttccctg tcgcaatata
    35821 tgccatcaga ccatgctcca gaaaactcag cgtggcatca aacatcgcta tatcgacatg
    35881 cgcccctctc tggctctttt cgcggccata aagtgcactc actattccgc tgaataaata
    35941 gacaccgcca cataaatccg caagagaggt acccacgcgc actggcggag catcagagta
    36001 ccctgtttcc atcattatcc cgctcattgc ctgaatgatg gtatcgtagg caggagcatc
    36061 ttttagcggg ccggtatgcc caaaacctga cgatgaagca tatatgagac gcgggttgat
    36121 ttcttgtagc gtttcccatg aaaaccccag tttttccatt gtacctgggc gaaaattctc
    36181 agctaataca tccgcttgtt taagcatatt tataaatatg cttttatcgt gatcattctt
    36241 taaatcaaga accacactct ctttgccatg attaataaaa ctgtaataga gtgactgtcc
    36301 atccacatag ggaccaaatg tgcgggtatc atcaccatga cctggcggct caactttaat
    36361 tacccttgcg cccatattac aaagaagttg agttccgaaa ggcccattaa ggacatgcgt
    36421 catatcgata actaataagc cttcaaacgg ccctttgcat tcattattgg tcatttgctt
    36481 ataccttgaa aaaagccgaa ggtgaaaaga cacttcggca tttatgtaaa atcaataaca
    36541 tgattaatta tgaaaccagt cgactgacat aaatccacaa gggagccgtg acgacaaaac
    36601 ccagtacgct cacagccaat gacgctgtac cagtgcgggt gtagacatta aaccgactgg
    36661 caataatgat tccggagaat gccggcggta atgcgcctgc cagtaccatc atctgcagat
    36721 gttcgctgtt caaatgacat gccataccca cgagaagcag cgccagtggc atcagaatca
    36781 gcttcaggaa ggtgttataa gcaatttcgg cactgaactc gaatttatgt gcagccagag
    36841 tcaaccccgc agcgaatacc gccacccctg agttagcctt cgcaatcaga ttaaaggttg
    36901 ggtcccatgc tgccggaatt tttaccccaa ccaacaccag gatcgttgcc agaacaggtg
    36961 cccatactac tggctccttt gccgcagaaa ttaatgcgct cagattacta ttcttcttac
    37021 catctgctcc tgaggaagga ttcagcaaat acagaccaat aggaatagta attgcgttaa
    37081 caataataga aataattgct acaactaaac ctgttgatac ggaatcacca taaataggat
    37141 cgagaactgc aaatcccagg aatccgatgg taggtgaacc tgcaattaat gcacatactg
    37201 ctgcttctgc atgagtacgt ttaaaaaatt tgtagcaacc aaaccaggag aaaaagaaac
    37261 atccgacaat aaccacaagt gatacaaggg tcaggcgagt gtccgcaaaa atcatttccc
    37321 tgtttgcccg ggtaatagaa acaaataggg ccgcaggaag agcgtagttt aataccagtt
    37381 tattaaatgc ccgggcttga tcttctgaaa aagtttctcg tctgccgcta aaataaccca
    37441 acagcatgat tacgataata ggcaataaat cgccaataaa aaatgttagc ataatagtat
    37501 ttccgataag ggtcccctca ccatcgataa tgagttggca agaggagacg atgtcagtat
    37561 ttaattacca gcgacttgtt ttgggttaag tttggtaata tggccacttt cagttcctgc
    37621 tgcagggtcg atgaccacat taataatggt cggtttgcgc gactgaatac cggtggttaa
    37681 agcatgacga agttcatctg tcgtagtgac gttatagcca acgccacgaa acgcatccat
    37741 taatttgtca taccttgcat ggtgcaatag atctgttggt gatggtgcac cagcgccact
    37801 gagatcaaca ccgtctcctc tgtagatgcc gccattatta aaaataacga tcgtcaccgg
    37861 caggttatat cgacaaatcg tttcaatctc catcccactg aaaccaaaag cactatcacc
    37921 ttcaatggcg acaaccggag aaccagaggt cacgctagca ccgatggcat agcccatacc
    37981 gatgcccatg acaccccagg ttccacaatc cagacggcga cgtggtttat acatatcaat
    38041 aatatttcgt gcattatcca gggtatttgc accttcatta actaaataaa tatcctggtt
    38101 ctcgcgcaat acatcgcgca cagcacttaa tgcattaaag taatttaatg gttgagtatc
    38161 tgtacttaat ttttcatgca ttttttgtgc attttgctgc ttgtggatat ttaaaatatc
    38221 gcgccatacc agtggagtcg taaatgtgtt ttgtttcagt tctgccagca taccttgcat
    38281 actggatgca atatcgccaa cgactggcac agcaatgggg cggttgctgt caatttcctg
    38341 cggttcaata tccagttgaa taaactgtgt atctgccgcc catccttttt taccgtgtgc
    38401 cagtaaccaa ttcagtcgtg caccaacaag catgacaacg tcagcatttg ccagggcaaa
    38461 cgaacgcgca gttgccgcag aaagtggatg tgtatcttcg aggatccctt tcgccataga
    38521 cattggcagg aatggaatct gggcactttc aataaattca cgaagctgtt catccgcttg
    38581 tgaatacgcc gcgcctttgc caaggataac taatggtcgt tcagcttttg ctaaaagcga
    38641 aattgcgcta gcgactgact tcgggcatgg caataatgct ggcgacggat tttcaacttt
    38701 tacaatcgtg gttaacgctt cgtctttttc catcgtcgcg gccaggacat ttgcaggcaa
    38761 atcaagataa actccgccag ggcgacccga tactgagacc cggatagcgc gtgccaatgc
    38821 aatgccaaga tcctgcggct gattaacgcg aaatgctgct ttggcatacg gttttgccgc
    38881 attcatttgg tccagctctt cataatcacc ttgctgtagg tcgacgatcg cgcggtcgct
    38941 ggagccgcta atcatgatca tcggaaaacc atttaccgtt gcgttggcca atgcggtcaa
    39001 accattgagg aatcctggcg cagacactgt caggcagatc cctggttttt gggtaagaaa
    39061 accgcttgca gcagcggcat agcctgccga ctgctcatga cgaaaaccaa tataacgaat
    39121 gccttccgcc tgggcatggc gtgccatatc cgtcacagga atacctacaa caccataaat
    39181 agtgtcaata ttattctgtt ttaatgcttc aacgatgata tgcataccat ctgtcatttg
    39241 aagttgatct gacatgatta ataacctcga aatttttata aattgggagc cagaagcccc
    39301 cgttaatatc agatggcgtg gttttgcttc attgcagcaa tttcatcgtc gctataaccc
    39361 agctcctgca atacagcagc ggtatgttca cctaatagcg gcgcagcttt aatatccggc
    39421 gtaaaggcag agaatttcat tggacaacca actgtcagat attttccacg caatggttgt
    39481 tccacttcaa caacactgcc actttggcgc aaagagggat caagtgaaat ttctttcata
    39541 cttaaaaccg gtgcacaagg aatatcaaac tgagtcaaat aggccaccgc ttcatgttta
    39601 tcaatagtga cagtgtattt ttcgatttca gcaaaaatat cgaaaatatg tggctgtcgg
    39661 gcatgggctg tactgtatgc cggatcggta atccaatctg gtttgccgat ggctttacag
    39721 gtgttttccc agttttgctc ctgaatagtg aaataaatat aggcgttagg atcggtttcc
    39781 caacctttac atttcaggat ccagccaggc tgaccgccac cacccgcatt acctccgcgg
    39841 ggaactgcat caccaaatgt gccattcgga tactgcgggt attcttccag ataacccaat
    39901 ttatcgagac gctgctggtc gcgtaatttc acgcggcaaa ggttcaatac ggcatcctgc
    39961 attgacatgg tgactcgttg cccacgcccc gttttttcgc gatgcagcaa agcagcaagt
    40021 aaaccgatca gtaaatgcat tccggtgttg ctgtcaccca acgctgcagc gcttaccagc
    40081 ggcggaccgt cccaaaaacc cgtagtggat gccgcaccac ccgctgcctg agcaacgttt
    40141 tcataggctt ttacattcac ataaggcgaa cactcatcaa accctttgat cgaaccaaaa
    40201 atcagacgtg gattgatttc ttgaatatgc tcccaggtga agcccatgtg atcaatggcc
    40261 cctggatgaa agttctcgac taagatatca gcttcgcgga tcagcttttc cattacctct
    40321 ttgccttccg ctgttttggt atttaattca atagaacgtt tgttactgtt aagcatggtg
    40381 aagtaaagcg catcgatatc aggaatatct cgcagctggt gacgcgttac gtcaccaacg
    40441 ccgggacgtt caattttaat gacgtcagcg ccaaaccagg ccagcatttg agtacaagat
    40501 gggccagatt gcacaccggt gaaatcgaga actttaattc cttgaagtgg agttgacata
    40561 gcaataccta ttagttaatt aaatatgtgt taatttagcg tttatttaag acataacgcc
    40621 tctataaata atccgcaaaa tatcgtcgta gctgcactcg atatttttaa attcctgtat
    40681 caagtactga aaatcatttc atcgctactt ccaattcctt tctgcatggt aagtagtgag
    40741 gacgaaaaga agaatatgat aaatattggt tcgcggtaat cagagagagc cgcgacattt
    40801 gtgatgttat tcttacaact gtaagaagcg ccggtgattt acaagtaagg gtatgattac
    40861 gctttttaaa gcgtaccata aaacataaca taataaatga tttgtgatga cacgggatta
    40921 ttgcggttta ctttgaggaa gataaaatgc gagtttatag aaccgcgagc gccaaataat
    40981 tttatgacca caatctgtcg tattgtggtc ataaaaaatt gtcggctgga gggagttaaa
    41041 tcctttacct caatatactg cctgtcagca ttagttcaca aacagtgctt cgctatcgac
    41101 gatgattcca tcttccgcac ctttaagcgc caggtttgcc tcgtagtatt gctttttatc
    41161 aagcagtttc tgcgcatcag caagtgcatt acgagtctgt ttgagcggca tcagatattg
    41221 gttttccatt acgccaacac cggccaggcg aagctcttcc attgcgccct ttttatcccc
    41281 tttcgccatt ttttcgttgg caatttttat cgcggcttct ttttcgggtg tcgctacata
    41341 actctcagaa attccgatag atgcattaat gacaatatat tggtcatcat tgacattggt
    41401 ttttttacct ggcttagcga actttgccca ttcggtactg tcatctgaca acaaagctga
    41461 agcttcattg gtcagttctt ttgccttttc cggatcgcca tgaaacagag ccaggcgcgc
    41521 tacctgcacg tcacgcatcg catataaacc ttgttcagag atacgctctg ctgctgccgt
    41581 tttctgggtt tgctgaactt gctgagtttg ctgttgtgcc gctaccggtg cattatcagc
    41641 cgcccataca gttgaagatg ccagaattgc tgtgaccatc gtagccataa ttaaacgttt
    41701 catacttacc tcattcttta acagttttag aaaacgccgc catcatattg ggtgagcatg
    41761 aaatgagaaa tcagagagca acggtcattt tgaaagggtt atcttacagt tgtagttatt
    41821 atcgcaagaa gtattttagt cttacaattg taggaatatg cttcctgtcg cagagaacta
    41881 ttcttatata ccacaaagag atgtcattta ttatatcgat gtaaatactg attaattaaa
    41941 tgtaaataca gccataacat cgtaggttct cagtgagcca ttctcaggat aactttctat
    42001 gaaagtaaac ttaatgcttt ttagcttatt tttattggtc tctattatgg catgcaatgt
    42061 ttttgcattt tccatttctg gtggtggaag tgagacgagc tataaagaga cagaaaaaac
    42121 gtcagtgatg acgacaactc attctacaaa acttcagcca tcacaggcga ttttgtttaa
    42181 gatgagagaa gatgcgccac cattaaatct cacagaagac atcacgcccc cttttccgac
    42241 aaaggcgaat tatcttattc atcctgtgcg atagcgtctg tcacatggca ggtgaacaat
    42301 catatctgtt gatgatgtga gcacctgccc aaatgatcca tcctgaacta ctaccgcctg
    42361 ccatacccca ccataaagcg aatcatcaag ccacaagccg gggcaagaaa cataaatatc
    42421 cccgtcacca gttcattacc gcctgttaac cacatggtga agtagtaaat gagtgcagcg
    42481 aggatcacca tgccgaagaa agaagagacc gccgagacca cgttcagtac ctgaccgatg
    42541 tatccgctta cgcagacaat acacagggcg agaaacatcc ataaataaat catagctcaa
    42601 cctcctgccg cgctaaaaaa cctgtaactt cagtcaattt aatagtaaga caatccttaa
    42661 cctctggcaa tttttgctaa gcatcaatga aaacagcatg ttaaatgcaa gactgttgcg
    42721 tacggaaaaa tatttacttt gcacgattaa taatcagtag ctgaaagcag tcagccagtg
    42781 ataaagtaca gataccgcat tcatgctcct cgtggttatg tcctgactag tctttacact
    42841 ctttacagga accattgtcg tacatgatgg cccaaccaat tgagtgtacc gctgtcgaca
    42901 tggaatcttc tgcgatacaa caattcgtat ctacagaagg taactatgtt tccaaaatgc
    42961 aaattttccc gcgagtttct gcatcctcgc tactggctca catggtttgg gcttggtgta
    43021 ctctggcttt gggtacagct tccttatcct gttctctgct ttctcggcac gcgtattggc
    43081 gcaatggcgc gaccattcct gaaacggcgt gaatctatcg cccgtaaaaa cctggaactt
    43141 tgtttcccgc agcattctgc tgaagagcgc gagaagatga tcgccgaaaa ctttcgttca
    43201 ctcggcatgg cgctggtaga aaccggcatg gcgtggttct ggcccgacag tcgcgtacgc
    43261 aaatggtttg atgttgaagg gctggataac cttaaacgcg cacaaatgca aaatcgcggc
    43321 gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg gcggccgcgt gatgggactg
    43381 tgtcaaccaa tgatggctac ctatcgtcca cataataatc agctgatgga atgggtgcag
    43441 acccgtgggc gcatgcgctc taacaaagcg atgatcggca gaaataatct gcgcggcatt
    43501 gttggtgcac tgaagaaagg tgaagcggta tggtttgctc ccgatcagga ttatggtcgt
    43561 aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg tcgccacaac caatggtacg
    43621 tatgtccttt cacgtctctc tggcgcagcc atgttaaccg taacgatggt aagaaaagcg
    43681 gattacagcg gatatcgttt gtacatcacc ccagagatgg aaggctaccc ggcagatgaa
    43741 aatcaagccg ctgcctatat gaacaagatt atcgaaaaag agatcatgcg cgcaccggag
    43801 cagtacctct ggatccaccg tcgctttaaa acgcgcccgg tgggagaatc gtcgctatac
    43861 atttaacaaa ctgctctaac tctttccctc ctcaaatcag ccataataac ctcatggttt
    43921 ttatggcttt tttcttaagg ttttttcctt cgctgaatct ctaattatta ccgtcagaaa
    43981 ttaaactgtc gccgatatga gacacctgta agttactaaa agtaaataag aaaatgcctc
    44041 ttgtctcgtt tcagttttag gcgtacatta gcgccgtctg gagcagttta gctcagaatt
    44101 ctggggtgat gcccacggta aaatacagga cttttaaggt cgttttgccc ggtgtcaact
    44161 cactgtatca ggtgtaatga agtcattcag gcgtaacagt aattacgcgg agagatgtaa
    44221 agtgaaatat ttctttatgg gcatttcttt tatggtcatc gtttgggccg gtacttttgc
    44281 cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga gccatcaggc tcctgtttgc
    44341 atttttgagg tcggtgttta ttccgcgttt tcgtgaatat gtttgctgct ggcgggtagt
    44401 aaaccatcgg cacggaacat cgctttaatc ccacgaatcg cctgacgaat acggtcgcgg
    44461 ttttcaatca gtgcaaagcg aacatgcgtg tcgccgtagt cgccaaagcc aatccccgga
    44521 gagacacaga ccttcgcttc gttcagcagc ttcttggcaa attccagcga gcccatggca
    44581 gcatacggtt ccgggatttt cgcccagaca tacatcgaag ccttcggcat ttcgaccatc
    44641 cagcccgctt catgcagccc ttttaccagt acatcacggc ggcgtttgta ctgttcagca
    44701 atgtcgcgca cgcactgctg atcgccctcc agcgccgcaa tcgctgccac ctgcaacggc
    44761 gtaaaggtgc cgtaatcgtg atagctttta atacgtgcca acgcgctgac cagcgttttg
    44821 ttgccaacca taaagccgat acgccagccc gccatgttgt aacttttcga cagggtaaag
    44881 aactcgaccg ccacgtcgcg tgcgcccggc acctgcatga ttgacggcgc tttccagcca
    44941 tcgtagacga tatcggcata ggccaggtca tggaccacca gcacatcgta gcgttttgcc
    45001 agcgccacaa ccttctcaaa gaattccagt tccacgcatt gcgcggttgg gttcgacggg
    45061 aagccgagga tcatcatctt cggtttcgga taactttcgc gaatggcgcg ttccagttcg
    45121 ttgaagaaat cgacaccttc caccagcggc actgagcgta cctgcgcccc ggcaatcacc
    45181 gcgccgtaaa tatgaatcgg gtagcttgga ttaggcacca gtacagtgtc accatgatcc
    45241 agcgtcgcca gcatcagatg cgccagtccc tctttcgaac caatggtgac gatggcttct
    45301 gattccgggt cgatctcgac gtcgtagcga tcctgatacc agcgggaaat ggcgcgacgt
    45361 agccgcggaa tgccgcgtga cgtggagtaa ccatgcgtgt ccgggcgctg ggccaccgta
    45421 cataattttt cgacgatatg cggcggagtc gcaccgtccg ggttacccat actgaaatcg
    45481 ataatatctt cgccgcgccg acgcgcagcc attttcagtt cagcggtaat attaaaaacg
    45541 tagggcggga gacgatcaat gcgcgtaaag cgacgttcag ggcgagtgtc agccataatt
    45601 tcctcagatt aacgttagcg cccggaccgt ccgagcgacg ctgccacgat ggtggctgtt
    45661 ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt agaacatttt tatcaatggc
    45721 tttaaatgag gaactccact tcaaaaacag gaaatttatc tgcgctaaca agaaaattac
    45781 cactcatttt attaacatta taatttcaat aaatttacca agcagatgtg cattatcaag
    45841 agatgcattg ctaatacacc atttatcttt caatcccctt ttaaaattgt ggtttttcct
    45901 caaaaacggc ctgctggtca tcagccttca ggttacctat catagaggtt taatccttat
    45961 tcagagtcac ccgtgcacga aatattcaac atgctgctgg cggtcttcga tcgggcagcg
    46021 ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc tgtttcgcga actgttgcac
    46081 aagtcggcgc actccccaaa agagctgctc gctgttaccg ccattttctc gctgttcgcc
    46141 ctgttcagca cctggtccgg cgttcccgta gaaggctcgc tagtgaacgt tcgtattatc
    46201 gcggtgatgt caggcgggat tctgtttggc ccgtgggtag gcatcattac cggcgtgatt
    46261 gcgggtattc accggtattt aattgatatc ggcggcgtga cggcgatccc ctgctttatc
    46321 accagcattc tggcgggttg tatatcgggc tggatcaacc tgaaaatccc caaagcacag
    46381 cgctggcgcg tcggtattct cggcggcatg ttatgtgaaa cgttgaccat gattctggtg
    46441 attgtctggg caccaactac cgcgttgggg atcgatatcg tctctaaaat cggcattccg
    46501 atgatcctcg gtagcgtctg tatcggcttt attgtgctgc tggtgcaaag cgttgagggc
    46561 gaaaaagagg ccagcgccgc gcggcaggcc aagctggcgc tggatatcgc caacaaaacg
    46621 ctgccgctgt ttcgccatgt caatagcgag tcattacgca aagtttgtga aattatccgc
    46681 gatgatattc atgccgatgc ggtggcgatt accaataccg atcatgtgct ggcctatgtt
    46741 ggcgtgggtg aacacaacta tcagaatggc gatgacttca ttagcccgac tacccgacag
    46801 gcgatgaatt acggaaaaat catcattaaa aacaatgatg aagcccaccg cacaccagag
    46861 attcattcca tgctggtgat cccattgtgg gagaaaggcg ttgtgaccgg aacactgaaa
    46921 atttactact gccacgcgca tcagattacg tcatcattac aggaaatggc ggtcggtctg
    46981 tcgcaaatca tctccacgca actggaggtt tcacgcgccg agcagctacg tgaaatggca
    47041 aataaggcag agcttcgcgc cctgcaaagc aaaattaatc cccatttcct gtttaacgct
    47101 ttgaacgcta tttcatcgtc aatccgtctg aatccggata ccgctcgcca gctgatcttt
    47161 aatctgtcgc gttatctgcg ctataacatt gaattaaaag acgatgagca aatcgatatc
    47221 aaaaaagagc tgtatcaaat taaagactat attgccattg agcaggcccg ctttggcgac
    47281 aagctgacgg ttatctatga tattgatgaa gaggtgaatt gctgcattcc cagcctgctg
    47341 atccagccgc tggtggagaa cgccattgtc cacggtattc agccttgcaa aggtaaaggc
    47401 gttgtcacca tcagcgttgc agagtgcggt aatcgggtac gcattgcggt gcgggatacc
    47461 gggcacggca tcgatccaaa ggtgattgag cgggtcgaag cgaacgaaat gccgggcaat
    47521 aaaattggcc tgctgaatgt ccatcatcgc gtgaagttat tgtatggcga ggggctgcat
    47581 atccgccgcc tggaaccggg gacggaaatt gcgttttaca ttcctaacca acgcacccca
    47641 gtcgcctcac aggctacgtt attgctttga gccggagtga tattgtgaaa gtcatcattg
    47701 ttgaagacga attcctggca caacaggaac tgagctggct aattaaagag cacagccaga
    47761 tggagattgt cggcaccttt gacgacggtc tggacgtgtt gaagtttttg cagcataacc
    47821 gcgtcgacgc catttttctg gatatcaata ttccgtcgct ggatggcgtg ttgctggcgc
    47881 aaaacatcag ccagttcgcc cataaaccgt ttattgtgtt catcaccgcg tggaaagaac
    47941 atgcggtaga agcatttgaa ctggaagcgt ttgactacat tctcaaaccg tatcaggagt
    48001 cacgcattac cgggatgctg caaaaactgg aagcagcctg gcaacaacag cagaccagca
    48061 gtacgcctgc cgcgacggta acgcgtgaga atgacaccat taatctggtg aaagatgagc
    48121 gaatcattgt cacgccaatt aacgatatct attacgccga agcgcacgag aaaatgacct
    48181 ttgtctatac gcggcgtgaa tcctacgtaa tgccgatgaa cattaccgag ttttgcagca
    48241 agctgccgcc gtcgcatttt ttccgctgcc atcgctcatt ttgtgtcaat ctgaacaaaa
    48301 tccgcgagat cgaaccgtgg tttaataaca cctatattct gcggctgaaa gatctggatt
    48361 ttgaagtgcc ggtcagccgc agcaaagtta aagaattccg ccagttaatg catctttaat
    48421 cgggaacttt catgaaagca ccaggcttgc ccgccgatca acaatttttc gccgatctgt
    48481 tcagcggcct ggtgcttaac ccgcaactac tggggcgcgt ctggtttgcc agccagcctg
    48541 cctcattgcc agtgggcagt ttatgtattg attttccccg tctggatatc gtgctacgcg
    48601 gcgaatacgg caatctgctg gaagcaaagc agcaacgtat ggtggaagga gaaatgctgt
    48661 ttattccggc acgcgcggct aatttaccgg tcaacaacaa accggtgatg ctgttaagcc
    48721 tggtgttcgc tccgacctgg cttgggttat cgttttacga tagccgcacc acgtcgttgt
    48781 tgcatcctgc tcgccagatc cagcttccca gcctgcaacg cggtgaaggt gaagcgatgc
    48841 ttaccgccct cacccatctt agccgttcac cgctggagca gaatatcatt cagccactgg
    48901 tgttaagttt gctgcatctt tgccgcaacg tggtgaatat gccaccgggc aattcgcagc
    48961 cgcgcagcga ttttctctat cacagcattt gtaactgggt tcaggataat tatgcccagc
    49021 cgctcacccg cgagagcgtg gcgcagtttt ttaatatcac gcccaatcat ctgtcaaaac
    49081 tgtttgccca acatgggacg atgcgtttta tcgagtatgt tcgctgggtg cgaatggcga
    49141 aggcgaggat gattttgcag aaatatcatc tgtcgattca tgaagtggcg cagcgttgcg
    49201 gttttccgga tagcgattat ttttgtcgcg tcttccggcg tcagtttggt ctgacgccgg
    49261 gagagtacag cgcccgcttt cggggctaac ttcagaaggt taattccgtt tccagcagcg
    49321 tcaggatgct ttgcgcatct tgcgcggcga acagcgactg gcggaagttt ttattcacca
    49381 gtttacgcgc cagctgcgag aagactttca catgattcat cccttcgtta gcgcccagtg
    49441 tcagcatgat caccagttcg acttcgccca tttctgactg ccagtcaacc ggtttcacca
    49501 gccgggcaat gctgatactg gaatgacgga tccactgaga tttggtgtgc gggatcgcta
    49561 cgccaaaacc aacgccggtg gtaacaatct cttcccgctg ccagacatcc tcttccagct
    49621 cgaacggatg ttcagtacgc ccgttaacgc cgaggttgcc gcacagaaac tggatcgcct
    49681 gctctttatt actaacggcc tgatcaacga agatattctc cagcgccagc agcgggcgaa
    49741 cgtcttcttc cggcgtaaag gcggtgagta acgcttcaat ttcctgcgcg ctgcgacatt
    49801 cacacgcctg acgtgccagt tcccgacacg cctcgctatc cagttgacga agctggcttt
    49861 tcaccgccgg aatgcgcggg ctactcatac tcagctcatc caggcccagc ccaagcagta
    49921 gcggcagata acggctttca ccgcccagtt caccgcaaat tcctacccat ttgccccgct
    49981 gatgcgcagt ggtaactatt tgctgcaaca tgcgcaggaa cgatggcgta atcgggttat
    50041 atagcggcga tacacgcggg ttattacgat cgaccgcata cagatactgg gtcatatcgt
    50101 tagaaccgat actgaagaaa tcgacctcat cgcagaagtg atcgatgatg tagcacaccg
    50161 atggcacttc caccatgata cccagcgtaa tcgtctctgc atgacgcagg ccatcgcgct
    50221 taagctcaac gatcgctttt tggatctcgc ccttcaccca taagatctga tcgaggctgt
    50281 ggaccatcgg gatcatcaac tgggcgttgc cgaaactggc ggcgcgcaaa atagcccgca
    50341 gttgagtgcg gaacaggcca gcaaattccg ggtaaattcg taccgcacga tagccgagga
    50401 acgggttctc ttcctgggga atgttgaggt aaggaatgct tttatcgccg ccgatgtcca
    50461 ttgtgcggaa gataatcggc ttgtcgcccg cggccagtag cacctgctgg taggcttcaa
    50521 attgctcctg ctcgtccggc gcgctgtcgc gatccatata gagcatttcg gtacggaaca
    50581 gcccgacgcc ttccgcgccg ttggcaaacg cgccaggcgc ttccagagcg gtgccaatat
    50641 tcgccgcgat atcaatacgc ttgttatcac gggaataagc cagctgcgcg gcagcctgcg
    50701 cctgttgttt ttggcgttta tccgccagcg tctgcgcgac ctgataataa ccgctcaccg
    50761 cgtcattcgg gttaatcgcc agcacgccgc actgggcgtc aagcactgcc ggttgcccgg
    50821 cataacgggc aatcgcatcc agcggcaggc cgctcagtac cgggatcgcc gaggcgcggg
    50881 ccagaatcag ggtatgcgag gtgcgcccgg ttttctccag aatcatgccc gcaagatttt
    50941 ttaaatcgag gctcaaaaac tggcttgggg ttaaatcttc agccaccaga atagtcggtt
    51001 tttcaagcac cagattattg cgcggtttta gttccggcca ggtgatatgc agtaactgtt
    51061 cgctgatatc gcgaatgtcg ctgacccgct cgcgcagata atcgctggca gaagcagaaa
    51121 gtttggcaca aacctgctcc atattgctga tgatcgccgc ccccagcccc tgatgctgtt
    51181 ctgccatcag gtgacggata ttgcctgcaa attcatcatc ctgaatcagc gacaaatggg
    51241 cgctgaggat agttttgctt tcgccgtcac gctcacgcag ctgctggttc agttgctcgg
    51301 caagcgttgc caggctgtgc tccagccggg tggagtcttg cgcactggcc gggatggccc
    51361 gataactgtc gaggctgtcg ctctgtaaca gggtcagcgt acccacgccg acgccgcttg
    51421 ccagcacatt gccgtacagt aaatcaggat tcagacggct taatgaacgc ggcagcggat
    51481 gcgccgtcag ttctgcctgt gttggctgga tgctgtcgct gtcgataaag cgcacctgga
    51541 tgtactcttc cagcacgcgc cgcgcctgct cttcatcgct gccgctaatg ttcaggctgc
    51601 aactgtcatt aaataaagtg ccggtgccaa tcagcgccag cgaacttttc gcatcggcct
    51661 ttgcgttctg gcgatggtta ataaaagtga tttcgctttg ccactggctg cactgttctt
    51721 taagttccca cgccggacga gcatgaatac cgttaggcaa aggacagaga aattgaatcg
    51781 ttaacataac cgctccgggt taatcaggag atcatctgaa atccgtcagt tgaacaaccg
    51841 tctcacgcgt aagacgttga ataagggcag ataaaagttg ctgcatctgc aaaatgtcgc
    51901 ggcaatcggc aatcgatgcc gcacaatgtc cgtggcgcgt cgctggcccc atcaccaccg
    51961 tgggtacgcc ggtgccagtt aaatgcaccg ctccgccatc tgtgccgccg ttgctgaaca
    52021 tatccgcctg caacggtacg ccaatttctg ccgccacggt ttcaatccac gcggtaagtt
    52081 ttggcggcgc aatcagcgac ttgtcgctta ataccagcat cggcccgtta ccaatctggc
    52141 gatggttagc cgcgccataa tcaaagtttt tcgcccagca ggcggtatca agcacaatgg
    52201 cgacatccgg cgacaccgcg cgggtggcag ttttcccgcc gcgtaatccc acctcttcgc
    52261 tggaacttgc caccagccac acttccgcag gcagttcggc gtcgtgcagc tcacgcagta
    52321 acgtcaccag cagatagcaa ccgaggcggt catcaaaggc tttccccatc actcgctggt
    52381 ggggtagaac ctgaaaagtt gtatcaaagg tgacgcgatc gcctggacga attcccgcct
    52441 gcatcacttc gtcatacgag cgagcgccaa tgtccacgcg catggtgctg acgtcatttc
    52501 cctgccggtc gccatcaagc agaccgggaa ttttgcactc ttcacgggtg gtgatgcgca
    52561 ccggctgcag ctggcgggca gccatgcgca cgttaccaac tggcagcaca tcaatcgccc
    52621 cttcgcggga gatgctgcgc accataaatc ccacttcatc catatgcgca cagatcatca
    52681 ccttcggacc tgtcgattca ttgaggcgga tcagcaccga tcccagacca tcaaatcgca
    52741 cctccttttg caggcgatcc gcttcttcca gcaggatctg ccgcacttcc tgttccgagg
    52801 aggcgatcgc atctgcctcg ctcaacgctt ttaatagcga taaatccatt atgcctctcc
    52861 cgtgagcaac actgttttcg gcatggcgta gagcacttct gcgccttgcg gggtgaccag
    52921 cacaacatcc tcgatgcgca cgcccccttg ccctggcaaa taaatccccg gctccacggt
    52981 cagtaacatg cctggctgta gcgtcgtggt gtcccgcggt gaaaaacgcg gatcttcatg
    53041 gacttcaatg ccgatagcat gaccggtgtt atgaccgaaa tagtcgccat aaccggcttc
    53101 tgtaatcacc cggcgcgcgg cgtcgtcaac ctgctggcaa cgcacgccgg ggcgaatcgc
    53161 ggagattgcc gcgagctgtg cctgcaaaac aatctgatag acgtcaaaca gcgggtgaga
    53221 ttcggcgctc accccttcgc cattcaccag caaggtacgc gtcatatcag agcagtagcc
    53281 ctgatacagc gcaccgaaat cgagagtgac aaactcgccc gctgcaacaa tcttgtcgct
    53341 ggctttgccg tgcggtagcg ccccacgcca gccgctggcg acaatggtat cgaaagaggc
    53401 tttttctgcg ccctgctggc gcataaacca ctccagttca gcggctatct cgcgctcgct
    53461 catccccgcc tgaataaagc ggcgaatatg ctcagcaccg cgatcggcaa tcccacaggc
    53521 gaggcggatt ttctccacct cctctggcgt tttgatttgc cgcagcacat ccggcgtggc
    53581 gctaaccagt ttcgcattga gttcagactg ccagcggtgc gcggtttccc aacatacctg
    53641 ctggccttca aaaccgagcg tttgcaactg ctcatcggca atgatttgat tgacgatagt
    53701 ggtaagcgtg ttcgtcgcgt caagcaaatg cagctggtag ccttgcgtgc gggcttctac
    53761 atccgcgtaa tagcgcgagt ccaccagaat gtgcgcactt tcacggctaa tcagcacata
    53821 gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc tgccgtgagg agagaagcac
    53881 cgcatccagt tgttgcgcct taagccagtc acgcagcgaa gcgagtaatg tcatctggat
    53941 tttccttctt acaggctatc gattaacaat ttgcctttac ggaacatcat cagacgcagg
    54001 aacaccacca tcagcgcagt tatgactgcc cccagcgcga tccccgccat gtagacgccg
    54061 aggttagtga ccagcggcca tgcccagata gcggactccg ggaaccattg cactgcgccc
    54121 agccagactg cagcggttga gccgacaatc gcgccgacca tataggacgg aatggcggtg
    54181 atggggcttt ccagtgcaaa cggaatcgca ccttcactga tccccataaa ggccaggaac
    54241 atggcggttt taccctgcgg ataaagctga gcgttgaaca ggcgtttgcc ggttaaacgg
    54301 cggtcgataa tggtcgccag acccagacca atcggcggaa taacgatagc gatagaacgt
    54361 gcggtgaccg gtaatacgtg gtccgtggta aagctgaagg caacgaaacc agctgctttg
    54421 ttgatcgggc caccaaggtc gattgctgtc gccgcggcga tccccatcgc gtacatcagc
    54481 gcgcccttct cacctgcggc agtcagcacg gtacggatac cgccgttgat ccagccgcca
    54541 aagggggtga tgacgtagta catcgccagc atgacaaaaa tggcggaaag aatcggtagc
    54601 aaaaaagtgg ttttgaacgc taacaggaaa tccggtaact ggattttctg gttcatccat
    54661 ttcacaaggt aaccagcaac gatagaaata atcagggcac caatgaaggt ggacggcacc
    54721 ggcgaagagg tcgcccattg catagtgctg ggatcgaagt tcagcagttg cgtcggctgg
    54781 gtagacatca agccgccgat aaaaccagcc gggaacgcca gtttgccacc gatagagttc
    54841 gccacaaatg cagcaaacat cggaatggcg aaaccaaaca acacgccgcc gaaggattgc
    54901 gacagccagg caaatttcag taaggaaagg tcgaaaccgg agaatttccc gctattaaga
    54961 gcatccatga tgccgatatc agcaggaatt ttaagccagc tataagcaat cagctggctg
    55021 aaagcgagga tcacaccgcc cataattaag gtcggcacca tgcgtgaaat ccctgacatc
    55081 acatgctgcg ggagttcacc ccagaagctg cttttagagg cctgcggatt cttaactgcc
    55141 gctgccgcac cggatgcgcc aggcacaacg gttgcactgc gttttttaat ggccataaca
    55201 attatccctg tcgaatatta ctgttgttca gaggcaatca tctcttcgat ttctttgatg
    55261 atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt cataaacgtc gcgtgattcg
    55321 aaacgttcgt tatcttccgg ggtaaccgcc acggagtgga tgatgatggt cgcttcggcg
    55381 atatcctgcg cggtcagacg attctggata ccgtccgcac cctgggtttc aattttcact
    55441 tcataaccgg cttctaccgc cgcttcttcc agcgcctgag cggccataaa ggtgtgagcc
    55501 aggcccattg ggcaggcaca taaggcaatc agtttcttac tcatcgttaa ttcctcgttc
    55561 agtgatagtt gacgagtgaa attatgaacg tgcaaaacaa atcgccgtta ccagacaaat
    55621 aatgcattta ctggagaatt aatcccgctg cacggaagtg tgacgcaggt cggccttgtg
    55681 aggggacaca aaggaaaatt gaattaactg tatgatttaa aagattatcg ggagagttac
    55741 ctcccgatat aacaggaagg agttacagaa tgtggcctaa ggtctggcgt aaatgtgcgc
    55801 cggaaccgag aaggcccgga ttgtcatgga caatgagata caccggaata tcatggacat
    55861 attctttaaa gcgcccttta tcctcaaatg cggcacggaa accggaggct ttgaagaact
    55921 caaggaaacg tggcacgatg ccacctgcaa tataaacccc accgaaagtt cccagcgtca
    55981 atgcaaggtt gccgccaaaa cgacccataa tcacacaaaa tagcgacaac gcgcggcgac
    56041 aatcggtgca gctgtcagcc agcgcgcgtt cagtaatatc ttttggcttg agattttctg
    56101 gcaggcggtt gtcggctttc acaatcgcgc gatacaaatt caccagccca ggaccagaaa
    56161 gcacgcgctc tgccgaaaca tgaccaattt ctgcacgcag tatttcgagg ataatggcct
    56221 cttcttcact attcggcgca aaatcaacgt gaccgccttc gcctggcaaa cttacccaac
    56281 gcttatcgac atggaccaga tgcgcaaccc caagccccgt tccggcaccg taaaccgcaa
    56341 taggcttacc ttcgaccggt tctgcgccac caaactgaat cagatgctct tttttcagca
    56401 tcgggatcgc catcgataca gcggtaaaat cgttaataat ttccagatgg ctaaaaccga
    56461 gattcttttt catttcggca attgagaacg cccaggtatg gttggtcatt gccacccagt
    56521 caccggtaat tgggcaagcg atggcaatac agccgtcttt cacctcgacc ttatgttctt
    56581 caagataaac gcgaatgacc gcttcgaggc tggggtaatc aagccctgaa taggtcttag
    56641 cctgcgagat ttcaccactg gcaatatcac acagagcaag acgtgcgttg gtgccgccca
    56701 catcaccgac taatgcatac tttgtcattc ttcaactgct ccgctaaagt caaaataatt
    56761 ctttctcaca ctgtaaatac ctgggggcat aacaacaacg ccggaaaggc aggctccctg
    56821 taaatatcga tctgggtcac acaattactt tatcgtttca gcaccaattg cagcgatgcc
    56881 tttttgcaag ctgggcaaac taagtatctg accccgcata aggaatagaa catgctccat
    56941 ccgcgagcca gaaccatgtt gttattatcg ctccccgccg tggcaattgg gattgcgtcc
    57001 agtcttattc tgattgtggt gatgaaaatc gcctcggtat tacagaattt gctctggcaa
    57061 cgactgccgg gaactctggg gatagcccag gattcacctc tctggatcat cggtgtatta
    57121 acgctaacgg gtattgcagt ggggttggtt atccgtttca gccagggtca tgccggacca
    57181 gaccccgcct gtgaaccgct gatcggcgca ccagttccgc cctctgcgct accaggactt
    57241 atcgtagcat taattcttgg tcttgctggt ggcgtcagcc tggggccaga acatccgatc
    57301 atgaccgtca atatcgccct tgcggttgcc attggcgctc gtctgttacc gcgcgtcaac
    57361 cgaatggagt ggactatttt agcctctgcc ggaaccatcg gcgcgctgtt tggcacacct
    57421 gttgcggcgg cgttgatatt ttcgcaaacc ttaaatggta gtagtgaagt tccgctatgg
    57481 gatcgtcttt ttgcgccgtt aatggcggca gcagctggtg cacttactac cggattattt
    57541 ttccatcccc atttttcact gcccattgcg cattacggac aaatggaaat gaccgatatt
    57601 ctcagcggtg caattgtcgc ggcgattgcc atcgcagcag ggatggtcgc cgtatggtgc
    57661 ttaccacggt tgcacgcgat gatgcatcaa atgaaaaatc cggtgctcgt gctgggtatt
    57721 ggcggattta ttctcggtat tctgggggtt attggtggac cagtttcgct gtttaaaggg
    57781 ctggatgaga tgcagcagat ggtggcaaat caggctttca gcaccagcga ttactttttg
    57841 ctggcggtaa ttaaacttgc cgccctggtc gttgctgccg ccagtggctt tcgcggtggg
    57901 cgaatcttcc cggcagtgtt tgtcggcgtg gcattagggt tgatgctgca tgagcacgta
    57961 cccgccgttc cagcggcaat aaccgtttcc tgcgctattc tcggcatcgt gctggtggta
    58021 acacgcgatg gctggttaag tctttttatg gcggcagtcg ttgtacccaa taccacattg
    58081 ctaccgctgc tctgtatcgt catgcttccg gcttggctgt tattagcagg taagccgatg
    58141 atgatggtca atcgtccgaa gcaacagcca ccccacgata acgtttagca aaaaatgctc
    58201 tcctttatga tttaagagtt atggcgatga tacggggctt tacctccccg taacattgcg
    58261 ttaacaggcc gctgacggct tcgatcgtga aggagaataa caatgttcag gtcactgttt
    58321 ctggcggccg ccctgatggc atttaccccg cttgcggcaa acgcaggtga aatcacccta
    58381 ctgccatcaa tcaaattaca aattggcgat cgcgatcatt acggtaatta ctgggacggt
    58441 ggtcactggc gcgaccgtga ctactggcat cgcaattatg aatggcgtaa gaaccgctgg
    58501 tggcgtcatg ataatggcta ccatcgtggc tgggataagc gtaaagcgta tgagcgtggc
    58561 tatcgtgaag gctggcgcga tcgtgacgat catcgcggaa aaggccgcgg acatgggcat
    58621 cgccattaaa cgcgtccgtc aatggagcac aatgcctgat gcgacgctag cgcgtcttat
    58681 cagacctaca aaccgtctct ttaacgcata gggcggataa ggcgttttac gccgcatccg
    58741 ccagtgatgc ctggtacgac gccagttgaa ctatagcccc agcgccgtac ccactaacaa
    58801 ccagatattc agagccacta ccagcaccac aatcacccag cctgtctgtt ttacgcgttt
    58861 gctgttcacc agatcgccca tcaacttgct gtcactggtg aaaatcagca gtggaaccag
    58921 cgccagggcg ataccaaaac ttaacaacac ctgactcata accagaatcc gtgtcggatc
    58981 taatcccatc agaatgacaa taaatgatgg caacatagtg actgtacgac gcacccacag
    59041 cgggatatga aagcgaatga agccctgcat caccacctgc cccgccagtg tccccaccac
    59101 cgttgaagac agtccggcag caaccaggct taatccaaag accgttgccg cagcatggct
    59161 taacaacggt tgcagcgtca gataagcctc atcaagatcg gcaacaccag tatgaccaga
    59221 aaagtggaac gccgccgcag ctgtagccat catcgccaga ttgacaaaac cggcaatagt
    59281 catggcgata gccacatccc atttggtggc ggaataacgt tgttgacgcg aaccgccatg
    59341 taaatgctga gtgagcgaag agtgcaaata aatcacatgc ggcataatcg tcgcccccaa
    59401 cacgcctgct gccaggaaga ccgcctccga agtaggtaaa ctcgggatca ccattccttt
    59461 acccagctgc gccaggttag gctgggagaa aatcaactcg acaatgtaag ccgcggcaac
    59521 aaacaacagt aacccaccaa tcactttctc cagcggtttt tgcccgcgac gttgcagcat
    59581 taaaatcagg aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg aaacaccaag
    59641 aatgagttta aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg ccatcgcaat
    59701 aatttctgcc tgaacccaat agaaccacac tacgggacgc ggatagtgat cacgaatctg
    59761 ctccgccaga tttttaccgg tggcaatccc cagtttggca gagaggatct gaatcagcat
    59821 cgccatcagg ttggcccaaa cgacaaccca cagtagctga tagccgaagc tggcacccgc
    59881 ctgaatattg gtcgcaaagt taccgggatc gatataacca atcgccgcaa tgaacgcagg
    59941 tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg ctgctactct caacgcgata
    60001 gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg tttcatgcta tgccaaacga
    60061 gaatgattat caaattcatt taaatgggtt gtggtgattt ctctgataga ccaggattat
    60121 gactacgaaa agattgatga cttgaatgtt atggttatgt ttaatgttag cacatttaca
    60181 taactttcag cttccataca caacatagca gaaatgtatg acagctcact atttttgaag
    60241 cttgtcacag gacgtcatta tagtgtgtgt cagatctcgt tttccttaac catgttacat
    60301 agaatgtgca cggaaattta acctgcctca tatttggagc aaatatggac cgcgtccttc
    60361 attttgtact ggcacttgcc gttgttgcga ttctcgcact gctggtaagc agcgaccgca
    60421 aaaaaattcg tatccgttat gttattcaac tgcttgttat cgaagtgtta ctggcgtggt
    60481 tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg cttctccgaa atgttcgaaa
    60541 aactgctcgg atttgccaac gaagggacta acttcgtctt tggtagcatg aatgatcaag
    60601 gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt ctttatctct gcactgatcg
    60661 gtattctcca gcacattcgc gtgttgccgg tgattatccg cgcaattggt ttcctgctct
    60721 ccaaagtcaa cggcatgggc aaactggaat cctttaacgc cgtcagctcc ctgattctgg
    60781 gtcagtctga aaactttatt gcctataaag atatcctcgg caaaatctcc cgtaatcgta
    60841 tgtacaccat ggctgcaacg gcgatgtcca ccgtgtcgat gtccatcgtt ggtgcataca
    60901 tgaccatgct ggagccgaaa tatgtcgttg cggcgctggt actgaacatg ttcagcacct
    60961 ttatcgtgct gtcgctgatc aacccttacc gtgttgatgc cagcgaagag aacattcaga
    61021 tgtccaacct gcacgaaggt cagagcttct tcgaaatgct gggtgaatac attctggcag
    61081 gtttcaaagt ggccattatc gttgccgcga tgctgatcgg ctttatcgcc ctgatcgccg
    61141 cgctgaacgc actgtttgcc accgtgactg gctggtttgg ctacagcatc tccttccagg
    61201 gcatcctggg ctacatcttc tatccgattg catgggtgat gggtgttcct tccagtgaag
    61261 cactgcaagt gggcagtatc atggcgacca aactggtttc caacgagttc gttgcgatga
    61321 tggatctgca gaaaattgcc tctacactct ctccgcgtgc tgaaggcatc atctctgtgt
    61381 tcctggtttc cttcgctaac ttctcttcaa tcgggattat cgcaggtgca gttaaaggcc
    61441 tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct gaagctggtt tacggctcta
    61501 ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt gctgtaagac catacataaa
    61561 aaagccgggg ataattcccc ggtttttttt acgcctgtta atcagctaat ggctgcgggc
    61621 gaccaatcaa atacccttgc agatattgca ccccgagctt atgcaatagc gcctgctgct
    61681 gttgcgtctc gacaaactcc gcgaccacac tcaatgactt cgctttcgcc agatcggtaa
    61741 ttgatctcac aatcatcgca tccagcgtat tcgtgacgat atctttcaca aagacgccat
    61801 caattttgat gatatcagcc tgcaaacgct ttaaccgttc gtagttggca tagccggtgc
    61861 caaagtcatc aatcgcaatc cggaaaccaa acttatgcag ttgctcgatg ttgtacatgc
    61921 tgctttctgc gttagaaaac gcctgctcct cggtgatctc aagaatgacc gcctgcgggg
    61981 agatgtgata acgcttaaac agacgaataa tccgcccggc aatattcttt tgcagcagcg
    62041 tgagcggcat taaattgact gaaaagcgcg ggcctttttt gtcgcaagga tgtgttgcca
    62101 gccacttcaa cagggattcc agcacttgca aatcaaaacg cgcgctaagg ttaaactgag
    62161 caataagggg cagaaactta tccggggtca taatgccgcc gtcatatttc agtcgcgcga
    62221 ggatctcatc ataaccttca ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat
    62281 caccctgatc taacgccgta cgaatggtat tcagcaatag cacctgttta gtggtctgcc
    62341 cggaaaccat ctcctctctg ctatccagcg ccagcacatg gtgatgtgcg caggattgct
    62401 ccgccagcca gcttaactgc cccaacaagg gttgcagggt ttcctgattt ccatcaaaac
    62461 gcccccaggc agcaccatag cccatatcca gcccggtatt gttccaatga atttgccgac
    62521 tattcaggat gttaaccata tgctggagtc gcccttccgt ttccggcccg ctcagcacta
    62581 acagcagttc actacccggc aattgataca acttttcgtt ttcctgcatc aacggcagca
    62641 gcgtacggta aattgatcgg atacagtgaa cgcgcatcat taagccgtaa tgacgactca
    62701 taaactcaag attatcaatg cgcaggcagc aaaaactctt gcccgcctct tgctccggcg
    62761 cttgttctaa cgcacgaaag ttgggtagga gcgttaacgg atcggtcagc gcctgcaaat
    62821 gccagcggcg attaagccat tcactgcgat gataaatgcg caccatatag agcaggcaaa
    62881 cgctaaagga aatcagcacc gcgagaataa atgccagcga atattcggtt agtacccctt
    62941 gcaaaaagtt ctggttgtaa ttcagcaggc aaagcgttga aacagcccag gtgagattta
    63001 aaaacggata gcgaagctta ccgacaccga gggtaaagat gataaagaaa acaggtacca
    63061 ggtaaccggc aataaagtca ttctcatacg gtgtgcacat cagaagtagc agcacgctaa
    63121 gagctgccag ccagcttaag gtaaatgcgc gtttctcttt gctcaacgac ggagcgatat
    63181 ccctgcgcca caatatctgc gcaaagtggg gacttacaat catgcgagtg agatagtaga
    63241 aaagcatgtt gtaaatcagc acagcggtga aaaggcttag taaatcaacg actgtgaaaa
    63301 tggcatccgc atcgccgaaa aaggtggata tctttagcgg gaaatcaaaa aagcttccca
    63361 caagatacat gctgcatttg atgccgatcg gcgtcaccaa accaagccag aaaagacgtt
    63421 gccagacata tcggctggtc aatccataac gccaacgcgt ccccagctgc cagcgtaata
    63481 ttgcgcaggc acacaacaca acaaacgtct ggctgccgag catgaccgcg gtctgtaaaa
    63541 aagagagatg aaagttccac agattggtgc aaaacatccc gcataaaacg ggaacgacac
    63601 cgcgccagcc aaagataaac aagattgaca gcattacgca cagtggcatc cacgccagaa
    63661 agatataact ggaatggata atggcaagtg gcgaaataaa acgggatagc tgaataagta
    63721 ccacggtgag cgtaaacgct agtgtgaata tcttgatatt ttttatcagg ttatgctcca
    63781 caaacatgag tacactactc tcactgatcg gggttttcag gcacacaaat aataagttta
    63841 ttcgcatcgc gaagcaaaca ggggccagcc tgttgccgat ttttcatcca atgatgcttt
    63901 caccaggcgg cgctaacccg gacgtagact gtattacaaa agcggcaaaa agcagagaca
    63961 aaaaaccccc gctttacagc gagggtttga aatttggtgg agctaagcgg gatcgaaccg
    64021 ctgacctctt gcatgccatg caagcgctct cccagctgag ctatagcccc acgaggcgtt
    64081 tacgtaccaa atttgctggg tgcaaaattt ggtggagcta agcgggatcg aaccgctgac
    64141 ctcttgcatg ccatgcaagc gctctcccag ctgagctata gccccgtcac gtaaagcttg
    64201 tcgagttgac gggcggcatc atatgaattc cgcccgaatg tgtcaacggc aaattgcaac
    64261 gtgtagtttc aatcgctgaa aaatcaggca aatgaacaat tttggaaaac cgctcgcatt
    64321 cagtagttat tcatgtcacg gtttcctgta aagtggtgtt ataaaatgaa ctactaatag
    64381 acccacatac attcagggaa ttgttatgtt caaggagagg atgacgccag atgaacttgc
    64441 cagactgacc ggctatagcc gccagaccat taataaatgg gtacgcaagg aaggctggac
    64501 aacgtcacca aaacctggcg tccagggtgg caaagcccga ctggttcacg tcaatgaaca
    64561 ggttcgtgaa tatattcgca atgctgaacg tccagaaggt cagggagaag cgcctgccct
    64621 gtctggtgat gcgccgcttg aagtgttgct ggtgacactg gcgaaagaga tgacgccagt
    64681 tgaacaaaaa cagtttacat ccttgcttct gcgggaaggg attatcggat tattacaacg
    64741 cttagggatt cgcgatagca aataatatga aaagattacg caataaaatg accaccgaag
    64801 aactggctga atgcctcggt gtggctaaac aaactgttaa ccgttggatc agagaaaaag
    64861 gctggaaaac ggaaaaattt cctggcgtga aaggcggtcg tgccagactt attctggtcg
    64921 atacgcaagt ttgcgagttt attcagaaca cgccagcctt ccataacacg ccgatgcttc
    64981 tggaagccga agaacccctt gccgaatacg caccggggat tcgcactcct gcttatcggc
    65041 aaatcatcag cgcaatagac aacatgactc acagtgagca ggaaaaagtt gcgcaatttt
    65101 tgtcacgcga aggaattcgt catttccttg ctcgtctcga catagatgaa tcagaataaa
    65161 taaaaaacgg caggggtatc tctcctgccg tttatctttt atgcgctaat tactgctgat
    65221 tttcgcgttc agcaataaaa gccagcgctt tgttgatacg ctcgatactg cgggtcttac
    65281 cgattgcgtg aacggtaaca tccagtgccg gagactgccc cgcaccggta acggcgacac
    65341 gcagcggcat accaacttta cccataccca cttccagctc atccgccgtc gcctgaatag
    65401 cgtgatgaac gttttcagtg gtccagtcag taatcgcggc cagtttgtca cgaaccactt
    65461 ccagcggctg acgcgctacc ggacgcagat gttttttcgc ggcgtcggca tcgaactcag
    65521 caaaatcttc gtagaaataa cggcagctct gtgccatctc tttcagcgtc ttgcagcgtt
    65581 cgcccagcag tttcaccaga tcagccagct gcgggccgtt acgggtatcg atattttcct
    65641 gctcaatgtg ccactgtaag tgagtagcaa catactccgg cggcagcgcg ttaatgtagt
    65701 gatggttcag ccacagcagc ttgtcggtgt tgaacgcact ggcagatttg ctgacggcat
    65761 tcagggtgaa gtatttgatc atctcttcac gagtgaagat ttcctgatcg ccgtgggacc
    65821 agcccagacg caccagatag ttcagcagtg cttctggcaa ataaccatca tcacgatact
    65881 gcattacgct gaccgccccg tgacgtttgg acagtttttt gccgtcatcg ccgttgatca
    65941 tagaaacgtg cgcgtaaacc ggtaccggcg ctttcagtgc tttaagaatg ttgatctggc
    66001 gtggcgtgtt gttgatatgg tcttcgccac ggataacgtg ggtgatttcc atatcccagt
    66061 catcgacaac cacacagaag ttataggttg gagaaccatc ggtacgacgg ataatcagat
    66121 catccagctc ctggttgctg aattcgatcg gaccacggat ctgatcgtca aaaacaacag
    66181 aaccttcctg cgggttagca aaacgtacaa cacacggttc atcatcagca tgatgctcat
    66241 ggctgtggcg gcagcgaccg tcataacgcg gcttctcacc tttcgccatt tgctcttcgc
    66301 gcagcgcttc caggcgctct ttagagcaat agcatttata agctgtgccc tcttccagca
    66361 tctgatcgat caccgcgttg tagcgatcaa aacgtttggt ctggtagtac ggaccttcat
    66421 cccactccag gctcagccag ttcatgccat ccataatggc ttcgatagct tccggcgtgg
    66481 aacgctcaag atcggtgtct tcaatacgca gcacgaactc accgccgtga ttacgtgcaa
    66541 aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg cagatagcct gttgggcttg
    66601 gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt ttagaaagat gccgacaacc
    66661 ggcaaatcct ggaaaaattg agtgggcgat attctatcac tcccgcctga ttcctcaatg
    66721 tagttcgggt tgcaacctta cgcattattg ttttcgttga caaattgcgc aacacggtct
    66781 gttttgcgat catcttgctt aaatttacga cgaacgaaca atttctttag aaaatgcgtt
    66841 gactcatttt gaactctccc tataatgcga ctccacacag cgggggtgat tagctcagct
    66901 gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccaa
    66961 ctactttatg tagtctccgc cgtgtagcaa gaaattgaga agtgggtgat tagctcagct
    67021 gggagagcac ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac
    67081 tttctcgcca gctaaatttc ttgtaaaaat gtgaagtacc gaagtgggtg attagctcag
    67141 ctgggagagc acctccctta caaggagggg gtcggcggtt cgatccc
//
LOCUS       DAPOZO010000012        66924 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPOZO010000012 DAPOZO010000000
VERSION     DAPOZO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.012"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..277)
                     /gene="ihfA"
                     /locus_tag="ROI21_000824"
     CDS             complement(<1..277)
                     /gene="ihfA"
                     /locus_tag="ROI21_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDX5109783.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVE"
     gene            complement(282..2669)
                     /gene="pheT"
                     /locus_tag="ROI21_000825"
     CDS             complement(282..2669)
                     /gene="pheT"
                     /locus_tag="ROI21_000825"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDX5109784.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(2684..3667)
                     /gene="pheS"
                     /locus_tag="ROI21_000826"
     CDS             complement(2684..3667)
                     /gene="pheS"
                     /locus_tag="ROI21_000826"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDX5109785.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(3732..3856)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(3806..3850)
                     /gene="pheM"
                     /locus_tag="ROI21_000827"
     CDS             complement(3806..3850)
                     /gene="pheM"
                     /locus_tag="ROI21_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDX5109786.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(3973..4329)
                     /gene="rplT"
                     /locus_tag="ROI21_000828"
     CDS             complement(3973..4329)
                     /gene="rplT"
                     /locus_tag="ROI21_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDX5109787.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(4383..4580)
                     /gene="rpmI"
                     /locus_tag="ROI21_000829"
     CDS             complement(4383..4580)
                     /gene="rpmI"
                     /locus_tag="ROI21_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDX5109788.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(4677..5219)
                     /gene="infC"
                     /locus_tag="ROI21_000830"
     CDS             complement(4677..5219)
                     /gene="infC"
                     /locus_tag="ROI21_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDX5109789.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(5223..7151)
                     /gene="thrS"
                     /locus_tag="ROI21_000831"
     CDS             complement(5223..7151)
                     /gene="thrS"
                     /locus_tag="ROI21_000831"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDX5109790.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            7674..9572
                     /gene="espL1"
                     /locus_tag="ROI21_000832"
     CDS             7674..9572
                     /gene="espL1"
                     /locus_tag="ROI21_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HDX5109791.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSNIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRSLL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSVYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADMYVRNCAIAFLPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPITQELLSKKMVMDILAMKTRDGEPGLYAAMENNHP
                     LCVTRFLSKVYGIAVKYNLSKINIMDLLKGATAHGTPALYIAMSKGNKDVVLSYISTL
                     GTFAKKYSFSQCQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     ADELKTYL"
     gene            9739..9846
                     /locus_tag="ROI21_000833"
     CDS             9739..9846
                     /locus_tag="ROI21_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109792.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(9899..10657)
                     /locus_tag="ROI21_000834"
     CDS             complement(9899..10657)
                     /locus_tag="ROI21_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDX5109793.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            10944..11873
                     /gene="pfkB"
                     /locus_tag="ROI21_000835"
     CDS             10944..11873
                     /gene="pfkB"
                     /locus_tag="ROI21_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDX5109794.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALSEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            11974..12264
                     /gene="ghoS"
                     /locus_tag="ROI21_000836"
     CDS             11974..12264
                     /gene="ghoS"
                     /locus_tag="ROI21_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDX5109795.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            12370..13230
                     /locus_tag="ROI21_000837"
     CDS             12370..13230
                     /locus_tag="ROI21_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDX5109796.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(13271..13807)
                     /locus_tag="ROI21_000838"
     CDS             complement(13271..13807)
                     /locus_tag="ROI21_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDX5109797.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            13954..14622
                     /gene="hxpB"
                     /locus_tag="ROI21_000839"
     CDS             13954..14622
                     /gene="hxpB"
                     /locus_tag="ROI21_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDX5109798.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            14785..15375
                     /locus_tag="ROI21_000840"
     CDS             14785..15375
                     /locus_tag="ROI21_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDX5109799.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            15508..16899
                     /gene="tcyP"
                     /locus_tag="ROI21_000841"
     CDS             15508..16899
                     /gene="tcyP"
                     /locus_tag="ROI21_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDX5109800.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPEFIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(16903..17718)
                     /locus_tag="ROI21_000842"
     CDS             complement(16903..17718)
                     /locus_tag="ROI21_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109801.1"
                     /translation="MRREMFSTNLIQNDYGNLNIKNLAFDSFKERLQSTMTALTFFIS
                     TGQCACDEIAESNFNYMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGS
                     EIRIRGINENFVGMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEQNYIHPVGSIFA
                     AIQKNESLLKFPSVISMLNVNLLFNPLNLPGMGSGVLEEIMSISDSSLRKRLGYEVLS
                     FSLQAHSLSQECIDKLAIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(17995..18258)
                     /gene="cedA"
                     /locus_tag="ROI21_000843"
     CDS             complement(17995..18258)
                     /gene="cedA"
                     /locus_tag="ROI21_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDX5109802.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            18441..20702
                     /gene="katE"
                     /locus_tag="ROI21_000844"
     CDS             18441..20702
                     /gene="katE"
                     /locus_tag="ROI21_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDX5109803.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPATEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVSDQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(20749..21507)
                     /gene="chbG"
                     /locus_tag="ROI21_000845"
     CDS             complement(20749..21507)
                     /gene="chbG"
                     /locus_tag="ROI21_000845"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDX5109804.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQSLSKDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKYAIAERGYRLGNYLDV"
     gene            complement(21520..22872)
                     /gene="celF"
                     /locus_tag="ROI21_000846"
                     /gene_synonym="chbF"
     CDS             complement(21520..22872)
                     /gene="celF"
                     /locus_tag="ROI21_000846"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDX5109805.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(22977..23819)
                     /gene="chbR"
                     /locus_tag="ROI21_000847"
     CDS             complement(22977..23819)
                     /gene="chbR"
                     /locus_tag="ROI21_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDX5109806.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(23827..24177)
                     /gene="chbA"
                     /locus_tag="ROI21_000848"
     CDS             complement(23827..24177)
                     /gene="chbA"
                     /locus_tag="ROI21_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDX5109807.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRIALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(24228..25586)
                     /gene="chbC"
                     /locus_tag="ROI21_000849"
     CDS             complement(24228..25586)
                     /gene="chbC"
                     /locus_tag="ROI21_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDX5109808.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPLVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(25671..25991)
                     /gene="chbB"
                     /locus_tag="ROI21_000850"
     CDS             complement(25671..25991)
                     /gene="chbB"
                     /locus_tag="ROI21_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDX5109809.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(26290..26628)
                     /gene="osmE"
                     /locus_tag="ROI21_000851"
     CDS             complement(26290..26628)
                     /gene="osmE"
                     /locus_tag="ROI21_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDX5109810.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            26830..27657
                     /gene="nadE"
                     /locus_tag="ROI21_000852"
     CDS             26830..27657
                     /gene="nadE"
                     /locus_tag="ROI21_000852"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDX5109811.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            27887..28774
                     /gene="cho"
                     /locus_tag="ROI21_000853"
     CDS             27887..28774
                     /gene="cho"
                     /locus_tag="ROI21_000853"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDX5109812.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPFSRGRSCFRSALKRCAGACCGKESHDDHALRLRQALERLRVVCW
                     PWNGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(28734..29309)
                     /gene="ves"
                     /locus_tag="ROI21_000854"
     CDS             complement(28734..29309)
                     /gene="ves"
                     /locus_tag="ROI21_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDX5109813.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(29512..29997)
                     /gene="spy"
                     /locus_tag="ROI21_000855"
     CDS             complement(29512..29997)
                     /gene="spy"
                     /locus_tag="ROI21_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDX5109814.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(30326..31294)
                     /gene="astE"
                     /locus_tag="ROI21_000856"
     CDS             complement(30326..31294)
                     /gene="astE"
                     /locus_tag="ROI21_000856"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDX5109815.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPMEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(31287..32630)
                     /gene="astB"
                     /locus_tag="ROI21_000857"
     CDS             complement(31287..32630)
                     /gene="astB"
                     /locus_tag="ROI21_000857"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDX5109816.1"
                     /translation="MNDWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFT
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDLGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRSRVNSFIAIEVPAAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     VLNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(32627..34105)
                     /gene="astD"
                     /locus_tag="ROI21_000858"
     CDS             complement(32627..34105)
                     /gene="astD"
                     /locus_tag="ROI21_000858"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDX5109817.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISDQAAQQVVTAW
                     QQLEAMGGRPLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDTFDEA
                     VRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSEEVVR"
     gene            complement(34102..35136)
                     /gene="astA"
                     /locus_tag="ROI21_000859"
     CDS             complement(34102..35136)
                     /gene="astA"
                     /locus_tag="ROI21_000859"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDX5109818.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGFLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(35133..36353)
                     /gene="astC"
                     /locus_tag="ROI21_000860"
     CDS             complement(35133..36353)
                     /gene="astC"
                     /locus_tag="ROI21_000860"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDX5109819.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            36799..37605
                     /gene="xthA"
                     /locus_tag="ROI21_000861"
     CDS             36799..37605
                     /gene="xthA"
                     /locus_tag="ROI21_000861"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDX5109820.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     KEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            37772..38482
                     /locus_tag="ROI21_000862"
     CDS             37772..38482
                     /locus_tag="ROI21_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDX5109821.1"
                     /translation="MNAERKFLFACLIFALLIYAIHAFGLFELLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIDKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTITFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGMALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            38487..39164
                     /gene="ydjY"
                     /locus_tag="ROI21_000863"
     CDS             38487..39164
                     /gene="ydjY"
                     /locus_tag="ROI21_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDX5109822.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDELSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            39179..39886
                     /locus_tag="ROI21_000864"
     CDS             39179..39886
                     /locus_tag="ROI21_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDX5109823.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            39886..40434
                     /locus_tag="ROI21_000865"
     CDS             39886..40434
                     /locus_tag="ROI21_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDX5109824.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSALAIPAQGLCRIPEKRS"
     gene            40444..41610
                     /locus_tag="ROI21_000866"
     CDS             40444..41610
                     /locus_tag="ROI21_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDX5109825.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNDAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWVETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQSPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEALDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFSEGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            41583..43118
                     /locus_tag="ROI21_000867"
     CDS             41583..43118
                     /locus_tag="ROI21_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5109826.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQTL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVTLWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVACQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            43118..43771
                     /locus_tag="ROI21_000868"
     CDS             43118..43771
                     /locus_tag="ROI21_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDX5109827.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     AALKGVARRDAVNDALERAGLGGMFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            43838..45145
                     /gene="ynjE"
                     /locus_tag="ROI21_000869"
     CDS             43838..45145
                     /gene="ynjE"
                     /locus_tag="ROI21_000869"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDX5109828.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDARLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(45154..45774)
                     /locus_tag="ROI21_000870"
     CDS             complement(45154..45774)
                     /locus_tag="ROI21_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDX5109829.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            45861..46268
                     /gene="nudG"
                     /locus_tag="ROI21_000871"
     CDS             45861..46268
                     /gene="nudG"
                     /locus_tag="ROI21_000871"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDX5109830.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEAAVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(46234..46506)
                     /locus_tag="ROI21_000872"
     CDS             complement(46234..46506)
                     /locus_tag="ROI21_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDX5109831.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            46741..48084
                     /gene="gdhA"
                     /locus_tag="ROI21_000873"
     CDS             46741..48084
                     /gene="gdhA"
                     /locus_tag="ROI21_000873"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDX5109832.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(48161..50116)
                     /gene="topB"
                     /locus_tag="ROI21_000874"
     CDS             complement(48161..50116)
                     /gene="topB"
                     /locus_tag="ROI21_000874"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDX5109833.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPGLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTESEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVIT"
     gene            complement(50121..51164)
                     /gene="selD"
                     /locus_tag="ROI21_000875"
     CDS             complement(50121..51164)
                     /gene="selD"
                     /locus_tag="ROI21_000875"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDX5109834.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKAAAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(51281..51832)
                     /locus_tag="ROI21_000876"
     CDS             complement(51281..51832)
                     /locus_tag="ROI21_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDX5109835.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSALLEQGAIAAGSDEKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            51993..53849
                     /gene="sppA"
                     /locus_tag="ROI21_000877"
     CDS             51993..53849
                     /gene="sppA"
                     /locus_tag="ROI21_000877"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDX5109836.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAIGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            54015..55031
                     /gene="ansA"
                     /locus_tag="ROI21_000878"
     CDS             54015..55031
                     /gene="ansA"
                     /locus_tag="ROI21_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDX5109837.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            55042..55683
                     /gene="pncA"
                     /locus_tag="ROI21_000879"
     CDS             55042..55683
                     /gene="pncA"
                     /locus_tag="ROI21_000879"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDX5109838.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(55776..57134)
                     /locus_tag="ROI21_000880"
     CDS             complement(55776..57134)
                     /locus_tag="ROI21_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5109839.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSVRLPPLTLNQSNSKANVINGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFIVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(57252..58010)
                     /locus_tag="ROI21_000881"
     CDS             complement(57252..58010)
                     /locus_tag="ROI21_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDX5109840.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(58147..59127)
                     /gene="ydjG"
                     /locus_tag="ROI21_000882"
     CDS             complement(58147..59127)
                     /gene="ydjG"
                     /locus_tag="ROI21_000882"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HDX5109841.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDQQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMESELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(59137..60084)
                     /locus_tag="ROI21_000883"
     CDS             complement(59137..60084)
                     /locus_tag="ROI21_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDX5109842.1"
                     /translation="MDNLDIICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRVGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSFVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(60089..60925)
                     /locus_tag="ROI21_000884"
     CDS             complement(60089..60925)
                     /locus_tag="ROI21_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDX5109843.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAKKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDTDIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(60946..61989)
                     /locus_tag="ROI21_000885"
     CDS             complement(60946..61989)
                     /locus_tag="ROI21_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDX5109844.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKAGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATDIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEEMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYQDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(62006..63385)
                     /locus_tag="ROI21_000886"
     CDS             complement(62006..63385)
                     /locus_tag="ROI21_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5109845.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSIFAIVSFGLLVCWSNAVGGL
                     ILAQLKELGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVVNK"
     gene            complement(63412..64488)
                     /locus_tag="ROI21_000887"
     CDS             complement(63412..64488)
                     /locus_tag="ROI21_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDX5109846.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNALRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(64858..65130)
                     /locus_tag="ROI21_000888"
     CDS             complement(64858..65130)
                     /locus_tag="ROI21_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDX5109847.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(65172..65585)
                     /gene="msrB"
                     /locus_tag="ROI21_000889"
     CDS             complement(65172..65585)
                     /gene="msrB"
                     /locus_tag="ROI21_000889"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDX5109848.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            65927..66922
                     /gene="gapA"
                     /locus_tag="ROI21_000890"
     CDS             65927..66922
                     /gene="gapA"
                     /locus_tag="ROI21_000890"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDX5109849.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
BASE COUNT        16823 a        17334 c        15933 g        16834 t
ORIGIN      
        1 tttcgacccg gctttttaac ttctgcccgg gtctgaaggt caccacgcgc cgtgctgtaa
       61 tgggaatatc ctcgcccgtt ttcgggttac gtcccgggcg ttgattctta tcacgcagat
      121 cgaagttacc aaaaccagag agtttcacct gttcgccgtt ttccagagcg cgacggatct
      181 cttcgaaaaa cagttcaacc agttctttgg catcccgctt gctaagccca agcttatcaa
      241 acagatattc tgacatttca gcttttgtaa gcgccatagg ttcaatccct caatgatgcc
      301 tggaatcgct cttttaatgc ctctacacat ttggcgacgg tagcggcaat ctcctcttct
      361 tcgagtgtac ggctggtatc ttgcaggatc aggcttatgg cgaggctctt atacccctcc
      421 gcaacaccct taccgcggta cacgtcaaat aagtttacgc caactacctg atttacgcca
      481 actttcttac attcggataa aatatccgct gcgggaacgt tttctgcgac caccaccgcg
      541 atgtcacgac ggttcgccgg gaagcgagaa atctcgcgcg cctgaggcac cacgcggtct
      601 gcgagcttgt tccactccag ttcgaacacc agagtgcgac cgttaagatc cagtttacgt
      661 tccagttcag gatgaacaac cccaacaaaa ccaatacgtt cacctttcag ataaatcgct
      721 gcggattgcc ccggatgcag tgccggattc gcttctgcac ggaactcaac ctcattcagt
      781 ttaccggtca ggtcgagaac ggattccaga tcgcctttca aatcatagaa atcaacggtc
      841 tcttttgcca ggttccagtg ctcttcgtaa cggttaccgc aaatcacacc ggctaacatc
      901 agatcctgac gaatgcccaa cggtgcctga gtatctggta cgaaacgcag accactttcg
      961 aaaatgcgca cacggttctg ctgacggttc tggttgtaca ccacggttgc cagcaggccg
     1021 gtccacaggg aaagacgcat tgctgacatt tcaacggaaa tcgggcttgg cagcagtaag
     1081 gcttcaacgc ctggatggat catctgctgc actttcggat caacgaagct gtaggtgatc
     1141 acttcctgat agcctttgtc gttgagcagc gttttcacgc gcttgagcga cagatctgct
     1201 tcgcggtgag tacccataat caggcttgcc tgcaccggct catcagggat gttgttgtag
     1261 ccgtaaacac gcgcgacttc ttcgaccaga tcttcttcaa tctccatgtc gaaacgccag
     1321 ctcggcgcaa ctgcctgcca ttcgtctttg ccttcggtca cttcgcagcc gagacgacgc
     1381 agaatatcag ttacctgctc atcggcaata tgatggccga tcaggcgatc cagtttgctg
     1441 cgacgtaaag taatggtggc tcgcttcggc agcgttgctt cgttggtgat atcaattacc
     1501 gggccagcct caccgccgca gatgtcgatc agcagacggg tcgcacgttc catcgctttg
     1561 tgctgcagtg ccggatcaac gccacgctca taacggtgag acgcatcggt atgcaggcca
     1621 tgacgacgag cacgaccggt gatagacagc gggctgaaga aagcgcattc cagcaggacg
     1681 ttttgcgttt cgtcattcac gccagagtgt tcgccaccaa agatgccgcc catcgccagc
     1741 gccttgttgt ggtcggcgat gacaagggtg tcagcattca gcttcgcttc agtgccatca
     1801 agcagcacca gcgtttcgcc ctctttcgcc atccgcacca caatgccgcc ttcaatgcga
     1861 tctttatcga aagcgtgcat cggctggccc agttcaagca gtacatagtt ggtgacgtca
     1921 acgactgcat cgatagatcg gatcccgcaa cgacgcagtt tttctttcat ccacagcgga
     1981 gttggcgctt taacgttaat gccttttacc acacggccaa gatagcgcgg gcaggcttcc
     2041 ggcgcttcga ctgtaatcgg cagcgtgtcg tcgatggtcg caccaaccgg aacgatttcc
     2101 ggttcaacca gcggcagctg gttcagcacg gcaacatcac gcgccacgcc gataatgcct
     2161 aagcaatcgg cacggtttgg cgttacgctg atttcgatgg tgttgtcatc gagtttcagg
     2221 tattcgcgga tgtcggtgcc aatcggcgca tctgcaggca gctcgataat gccgctgtga
     2281 tcgtcagaaa ttcccagctc agaaaaggag cacagcatcc cttcagacgg ttcgccacgc
     2341 agtttcgccg ctttaatttt gaaatcgccc ggcagaacag caccaatggt cgctaccgcc
     2401 acacgcaggc cctgacggca gtttggcgca ccgcagacga tgtccagcag gcgatcgccg
     2461 ccgacattca cttttgtcac acgcagtttg tcagcgttcg gatgctgcgc acactcaacc
     2521 acttcaccaa cgaccacgcc gtggaagcta ccggcaaccg gttctacacc gtcaacttcc
     2581 aggcccgcca tagtgatttg atttgccagc gcatcgctat caatcgccgg gttcacccat
     2641 tcgcgtaacc acagttcact gaatttcata atctattcct gccttattta aactgtttga
     2701 ggaaacgcag atcgttttcg aagaatgaac gcaggtcggt gacgccgtaa cgcaacatgg
     2761 tcagacgctc catccccatt ccgaaggcga aaccagagta aacttccggg tcgatgccaa
     2821 cattacgcag cacgttcgga tgcaccatcc cgcagcccag tacttccagc catttaccgt
     2881 ttttacccat gacgtccact tctgcagaag gttcggtaaa cgggaagtag gaaggacgga
     2941 agcggatctg caaatcttcc tcaaagaagt tacgcaggaa gtcgtgcagc gtgcctttca
     3001 ggttggtaaa gctgatgttg gtatcaacaa tcagaccttc catctgatgg aacatcggcg
     3061 tgtgagtctg gtcgtagtcg ttacgataaa cgcggccagg cgcgatgata cgaatcggtg
     3121 gctgctgagc tttcatggtg cggatctgta cgccagaggt ctgggtacgc agcaggcggg
     3181 tagcgtcaaa ccagaaagtg tcgtggtcag cgcgcgccgg gtggtgacca ggaatgttca
     3241 gagcatcgaa gttatgataa tcgtcttcga tttccggccc ggttgccacg gtaaagccaa
     3301 gctcaccgaa gaaactttcg atacggtcga tggtacgggt caccggatgc agaccgccgt
     3361 tttcaatacg acgccccggc agggagacat caatcgtttc cgccgccaga cgcgcattca
     3421 gcgctgcgct ttccagttcc gctttacgtg cattcagcgc ctgctgaacc tgctctttcg
     3481 cttcgttgat aaccgcaccg gctgccgggc gttcttctgg cggcagctca cgcagggtcg
     3541 tcatctgaag ggttaagtgc ccttttttac ccaaatattc gacgcgcaca ttgtctaacg
     3601 cggcaacatc tgacgcctgg ctaatggccg ccttcgcact ggcaaccagt tctgcgagat
     3661 gtgacatggt tttcctcatt gtgtcagtgg tgacactggt tcgttggact taattttttc
     3721 aaaacgcgca tacaaaaaaa gcctccactg ggaggctttc aggcgctgtt ttccgtttct
     3781 cttctcacgc gctagcctcc tggagtcagg tgctaaagta aaaaaagaag cggaaaatag
     3841 cagcattcat tgcttgcgtt accttttggt acttttcaaa agacctttat tgaaaaggct
     3901 acggcgataa aagtcaatgt tttgatggcg ttgaaacgaa aagagggaga ctagctccct
     3961 ctttcaactg gcttatgcca gagctgcttt cgctttttca accagagcgg tgaacgctac
     4021 tttgtcgaat actgcgatat cagccaggat cttacggtcg atttcaacag aggctttttt
     4081 caggccattg atgaatttgc tgtaagaaat accgttctga cgtgctgctg cgttgatacg
     4141 cgcaatccac agttgacgga actgacgctt acgttgacga cggtcacggt aagcatactg
     4201 accagctttg ataacagcct ggaaggcaac gcggtatacg cgagaacgcg caccgtagta
     4261 gcctttagct tgtttcaaaa ttttcttgtg acgtgcgcgt gcaataacac cacgttttac
     4321 gcgagccata tgtgctctcc tgtatctata ttctaattaa aaagttaaaa aacgttaacg
     4381 gcttatgcgt acggcaggca cgcgattacc aggcccagat cgcctttgga aaccatggct
     4441 ttcggacgca ggtgacgttt acgtttggtc gcttttttgg tcagaatgtg acgcaggtta
     4501 gcgtgcttgt gcttaaaacc acctttaccg gtttttttga agcgcttagc agcaccgcgt
     4561 acggtcttaa tttttggcat tttaataact tccacttcgc attgttaata aacgaaacaa
     4621 aggcgaacaa tgcctgtgaa gcccaaaggc tccacagaca gtgctacttg aaggccttac
     4681 tgtttcttct taggagcgag caccatgatc atctggcggc cttcgatctt cgttgggaag
     4741 gattcgacca ctgccagttc ttgcaaatcg tctttcacgc gattaagcac ttccataccg
     4801 atttgctggt gcgccatctc acgaccacgg aaacgcagcg tgattttggc tttatcaccc
     4861 tcttcgagaa agcgaatcag gctgcggagt tttacctgat agtcgccttc atctgtacca
     4921 ggacggaatt taatttcctt aacctggata actttttgct ttttcttctg ttccttagaa
     4981 gacttgctct tttcatagag gaatttgccg taatccatta tacgacaaac cggcggctcg
     5041 gcgttagggc tgatctcgac taagtctact ccggcttctt ctgctttctc cagagcttct
     5101 ctcagactca caataccaag ctgctcgcct tccagacctg ttaagcgaac ttcctgggcg
     5161 cgaatttcgc cattgatacg gttagggcgc gccgtttgaa ctcgttttcc gcctttaata
     5221 ccttattcct ccaattgttt aagactgcgg ctgcgaatct cttgttgcag cttctcgatc
     5281 acttcattta cgtccatgct tcccaggtct ttaccacggc gggtgcgaac ggcaactttg
     5341 cctgattcca cctctttatc accacagacc agcatatacg ggacgcgacg caaagtgtgc
     5401 tcgcggattt taaagccaat cttctcattt ctcaagtctg ctttaacacg aatgcccgca
     5461 tttgatagtt tttgcgtcaa ttcgttaacg tattcagact gtgaatcggt aatattcatg
     5521 ataacaacct gaaccggcgc aagccaggtc gggaagaaac cagcgaactc ttcggtcagg
     5581 ataccgatga aacgttccat cgacccaaga attgcgcggt gaatcattac cggtacctta
     5641 cgctcgttgt cttcgcccac ataggaggcg ctcagacgag acggcaagga gaagtccagc
     5701 tgtactgtac cgcactgcca tgcacgatcg aggcagtcat acagggtaaa ttcaattttc
     5761 ggaccgtaga aagcgccttc acccagttga tattcaaacg ggatgttgtt ttcttccagc
     5821 gcaaccgcca ggtccgcctc agcacgatcc cacatttcgt cgctgccaat acgtttttca
     5881 ggacgagtgg agagtttgac gacgatcttc tcgaagccaa aagtgctgta catatcatag
     5941 actaaacgga tacatccgtt aacttcatcg cgaatttgtt cttcagtaca gaagatatgc
     6001 gcgtcatcct gggtaaatcc acgtacgcgc atcaggccat gcagcgaacc tgacggctcg
     6061 ttacggtggc agctaccaaa ctcggccata cgcagcggca aatcgcgata cgacttcagc
     6121 ccctggttga aaatttgtac gtgacccggg cagttcatcg gcttaatgca gtattcacgg
     6181 ttctcagaag acgtggtgaa cattgcatct ttgtagttgt cccagtgacc ggttttttcc
     6241 cacaggacac ggtccatcat gaacggacct ttaacttcct gatactggta ctctttcagt
     6301 ttagaacgaa caaacacttc cagttcacgg aagatggtcc aaccgtcgtt gtgccagaag
     6361 accatacccg gcgcttcttc ctgcatatgg tacaggtcga gctgtttacc gattttacgg
     6421 tggtcgcgtt tcgcggcttc ttccaggcgc tgcaggtaag cgttaagtgc ttttttgtct
     6481 gcccacgccg taccgtaaat acgttgcaac attttgttgt tgctgtcgcc acgccagtaa
     6541 gcccctgccg ttttcattag tttgaaatga tggcagaaac gcatgttcgg tacgtgcgga
     6601 ccgcggcaca tatcgacata ttcttcatgg aagtacagac ctggcttgtc atcatgggcg
     6661 atgttttcgt caagaatgga gactttgtag ctctccccac ggttggcgaa agtttcacgc
     6721 gcttcgtgcc agctgacttt cttcttaatg acgtcgtagt ttttctcagc aagctcatgc
     6781 atccgcttct cgagtgcttc gacatcttcc tgggttaacg tgcggtcaag atcaacgtcg
     6841 taataaaaac cgttgtcaat aaccgggccg attgccattt tggtatgcgg ccaaagttgt
     6901 ttaatcgcgt gccctaacag gtgcgcacag gagtgacgaa tgatctccag accttcttcg
     6961 tctttggcgg taatgatcga cagttgtgcg tcgttttcaa tcagatcgca agcatcaacc
     7021 agttcgccat taacgcgccc tgcgatacag gctttcgcca gacctggacc aatgtccagc
     7081 gcaacatcca tggggcttac agcgtggtcg taatggcgtt ggctgccatc aggaagagtt
     7141 ataacaggca ttttatatcc ttatttgcag tggtgaccca cacgaaagat cacatacaaa
     7201 gaaaaatttg tttattaaca gttcattgcg aaaccatcta gccaacaaat gctaaattgg
     7261 ttcgcaatca ggtacacaaa tgaaagacat taacctctca ccttccaccc ggtggaacga
     7321 tgttaacact aaaaaaaggg agattgtacc tttccgtttc acattgattc gattcgcaat
     7381 tcgtttgtag tgagtacacc agcaaaacgc ttaaagaacc actgataaca tcacggagcg
     7441 tctgaaaggt ttactgcgag cggatttgca gcagcataaa ctgaatccag caaagttcgt
     7501 tgcgtgattt ggcggtacta tttaaacgat cataagatta atgacgaagt ggtcatatca
     7561 caatgataaa agtgacacaa ttcttatagc aatttttcgt gcacatttcg ttctggcgat
     7621 aataattaat catcatcctc aaaacctcca gacatatata aggcgaatag attatgagtc
     7681 aaaacgacat tattatcaga actcattata agtctcctca tagaatgcac atcgatagca
     7741 acataccaac gccttcatca gagcctatta atcaatttgc gcgccaactc atcaccctac
     7801 ttgatacctc tgacttaagt tcgatgctgt catactgtgt tactcaggaa tttaccgcaa
     7861 actgtcgaaa aatatcacaa aattgttatt ccactgccct ttttaccatt aactttgcca
     7921 cttcacccat ccatgcagaa aatatactca ttacattaca ctataaaaaa gaaatcattt
     7981 ccttattact ggaaaccacg cctattaaag ctaaccattt gcgaagcata ctggattata
     8041 ttgaacagga acagttaact gccgaaaatc gtaaccattg tatgaaactg tctaaaaaaa
     8101 tccatagaga aaaaactata caaccaacag taaatctcaa tggtagtgca ttttttttgc
     8161 aatctccttc tgacgctatt ttttgtcgcc atctgtcatt gcaatacgcc cttgattcac
     8221 tgagaaatgg aaaaggcaaa gtcaatctga ttaaacatta ctcctccgtt gaatccatac
     8281 agcagcatgt ccccttagtc cgggacgcgg agttcagatc attacttcgc catcctcctg
     8341 cagggagtcg cgttatcgcg agtaaggatt ttggcttcgc tttagatatt ttcttctgtc
     8401 gaatgatggc aaacaatgtc agtcatatgt ccgcgatttt atatatagac aatcatactt
     8461 tgtcagtaag gctacgaata aagcagtcag tgtatgggca attaaattat gttgtgtccg
     8521 tttacgaccc gaacgatacc aacgttgccg tcagaggcac ccacaggaca gcacggggct
     8581 ttctctcgct tgataagttc atcagttcag gtcccgatgc tcagacctgg gctgatatgt
     8641 atgttcgcaa ctgtgcaatt gcttttctgc ccctattacc tgagggagtt ccaggggcta
     8701 ttttcacggg tatcgcgaca cgaatgccat ttgcccctat acatccatcg gcaatgttgt
     8761 taataatggc cacaggccag actcaacagc ttattacatt attcaaacag ttacccatac
     8821 tccctgaaaa agaaatcatt gaaataataa ctgcgcagaa tagcgttggt acacctgctt
     8881 tatttctggc tatgatgaac ggacatactg acaacgtaaa aatatttatg caagaaattc
     8941 agtcactggt agataatcat atcattcatg aagataatct ggttaaatta ctgcaaacta
     9001 aaagtgctaa cgaaacacct ggactctata tctccatgtt gtatggattc gatgaaataa
     9061 tcgatatctt tctgaatgca ttaaccactc caataacaca agagctttta agcaaaaaaa
     9121 tggtgatgga tattttagca atgaaaacac gtgatggtga gccaggatta tatgccgcaa
     9181 tggaaaataa tcaccctttg tgtgtcacac ggttcctctc taaagtttat ggaatcgctg
     9241 ttaaatataa cctcagcaaa attaacatta tggatttatt aaaaggcgca acagcacatg
     9301 gaacccctgc tttatacatc gccatgagca agggtaataa agacgtcgtg ttatcttata
     9361 tatcaacgct gggtactttt gcaaaaaaat attcttttag tcaatgtcag ttattcacat
     9421 tgttggccgc taaaaatcat gacaacatgt cagctgttca tatagccatt catcataatc
     9481 attataaaac tgtagaaaca tattatgctg ctataaacgt aatcagccaa agcctgagtt
     9541 ttagtgctga tgaactaaag acgtatttat aaccagacat ttatatatcg atacataatt
     9601 ttattttttt cataaaccgc aaaaacagtg atcaggtgta caacgccacc cgacggctgt
     9661 aataacaaaa aatcccttga tgcctttccc ttttgttaca cttcgttatc acgcacaaga
     9721 gatatgcagg acactggtat gccgactaaa cgctttgata aaaaacactg gaagatggtg
     9781 gtggtgctac tggcaatttg tggcgctatg ttgttgctac gttgggcagc aatgatttgg
     9841 ggctgagcag tgacaaacga agacagccag accgcataac acggtccggc agataatttt
     9901 acatgctgta gcccagcgat aaggttgtcc gacgatcggt atgttccggt gcagactctg
     9961 gaggttcaga gttccatgtg acgttgtaag ccaccttcag tccaaaatgt tcattaatag
    10021 caacatttaa tgcgctttcg gagttcagcg ttgtgtcttc cgcgccaaag acggaaacac
    10081 cctgcgtaaa tttagcgttg tcagtcaact gccatgcata agccccggag gcataaccca
    10141 gcggctgggt ttcactggca ttatcggtat atttgtcgta acgcacacct ggaccgaatt
    10201 caaagcggaa actgtggacc gggccattca aaaactggcg accataaccc gcggtcaaca
    10261 catcgcgctc tcgatagccg ttataacggt ctgtcagcca gcttgcctgc ccaaataaat
    10321 agtcataatc agttaaatta aaacggctac gcccgcccgc ggcatatttt tctgaagaac
    10381 gctcatcatt agaagaagta ttactggcgt tcccccacag cgaccaggca gtggtttgtc
    10441 cataccaggt catggtggtg tcagccgtaa gtgaggagct tttcgtattg cctgattgtg
    10501 caagatatcc tgcgttcaga ttaccttcga aaggtttttt agcgctggca ggatcgtcca
    10561 tgacagtaaa aacggaatca tcggcagctg cattcaatga cgcaaacatg ccccccgcca
    10621 acataacgat ggcaggaact gtcttcaaaa gcttcattta tcaagagtcc gtacaacaaa
    10681 aaaagagacc atcgcggtcc cggaaacttt cttaaggatc aaagattagc gtccctggaa
    10741 aggtaacgaa ttataaaaag gcttgaataa cttagcaatg tattcttatt tcattttttg
    10801 aataagcatg tgaagaaaac agatttttat ttatatatat ttatctgcaa aattttaaat
    10861 aaagctctaa taaatcatat tgttaatttc ttccctttcc gctgattcgg tgccagactg
    10921 aaatcagcct ataggaggaa atgatggtac gtatctatac gttgacactt gcgccctctc
    10981 tcgatagcgc aacaattacc ccgcaaattt atcccgaagg aaagctgcgc tgtaccgcac
    11041 cggtattcga acccggaggc ggcggcatca acgtcgcccg cgccattgcc catcttggag
    11101 gcagtgccac agcgatcttc ccggcgggtg gtgcgaccgg cgaacatctg gtttcactgt
    11161 tggcagatga aaatgtcccc gtcgctactg tagaagccaa agactggacc cggcagaatt
    11221 tacacgtaca tgtggaagca agcggtgagc agtatcgttt tgtcatgcca ggcgcggcat
    11281 taagtgaaga tgagtttcgc cagcttgaag agcaagttct ggaaattgaa tccggtgcta
    11341 tcctggtcat aagcggaagc ctgccgccag gtgtgaagct ggaaaaatta acccaactga
    11401 tttccgctgc gcaaaaacaa gggattcgct gcatcgtcga cagttctggc gaagcgttaa
    11461 gtgcagcact ggcgattggt aatatcgagt tggttaagcc taaccaaaaa gaactcagtg
    11521 cgctagtgaa tcgcgaactc acccagccag acgatgtccg caaagccgcg caggaaatcg
    11581 ttaatagcgg caaggccaaa cgagttgtcg tttccctggg tccacaaggg gcgctgggcg
    11641 ttgatagtga aaactgcatt caggttgtgc caccaccggt gaaaagccag agcaccgttg
    11701 gcgctggtga cagcatggtc ggcgcgatga cactgaaact ggcagaaaat gcctctcttg
    11761 aagagatggt ccgttttggc gtagctgcgg ggagtgcagc cacactcaat cagggaacac
    11821 gcctgtgctc ccatgacgat acacaaaaaa tttacgctta cctttcccgc taactgaaac
    11881 attccccctg cattggggga atcaccacca acctgtcggc aacgcgtttc tccgactatg
    11941 ctcaaaagtc atgtgataac aaaggggtga actatggcca gtggcgatct tgtccgttat
    12001 gtcataaccg tcatgttgca tgaggataca ttgactgaaa ttaacgagtt gaataattac
    12061 ctgactcgcg acggtttttt gctcaccatg acggatgatg atggaaatat ccatgagctg
    12121 gggactaaca cttttggact tatcagtacc caaagtgaag aagaaattag agaactggtt
    12181 tcggggctta cccaaagtgc aaccggtaaa gatcctgaaa tcgccatcac gacctgggag
    12241 gaatggaata gcaacagaaa ataaatggtt tttgggcaat aatcagtctg tggtgtgctt
    12301 tagctcgtgt ttttacaccg cattcttgcg ctaaccttat gatctggcag acaacatggg
    12361 agagacatca tgtggcaggc aatcagtcgt cttttgagcg agcagttagg tgaaggcgaa
    12421 atcgaactgc gtaatgaact gcctggcgga gaagtccatg ccgcatggca tttgcgctat
    12481 gcaggacgtg actttttcgt caaatgtgat gaaagggaac tgcttcccgg ttttaccgcc
    12541 gaagccgacc aactggagtt actgtcgcgt agtaaaaccg tcaccgtgcc taaggtttgg
    12601 gcagttggcg ctgaccgtga ctacagtttt ctggtgatgg attatctccc acctcgcccg
    12661 ctggatgcgc atagcgcatt tattcttggt cagcaaattg cgcgtttaca tcaatggagc
    12721 gaccaaccac aatttggcct cgattttgat aactcgctct ccacaactcc gcagcccaac
    12781 acctggcaac gtcgctggtc aacgtttttt gctgaacaac ggattggctg gcagttggaa
    12841 ctagcagcag agaaagggat cgctttcggc aatatcgacg ccatcgtaga gcatattcag
    12901 cagcgtctgg cctcgcatca accgcagcct tctctgttgc atggcgattt atggtccggc
    12961 aactgtgcgc tgggtccgga tggcccgtac attttcgacc cggcctgcta ctggggtgac
    13021 cgagagtgcg acctggcgat gttaccgctg catactgaac agccgccaca aatctatgac
    13081 ggctatcagt cagtatcccc gctacctgcc gatttccttg aacgtcaacc ggtttaccaa
    13141 ctctacacgc tgttaaatcg tgcaaggtta tttggcggtc agcatttggt tattgctcag
    13201 cagtcactgg atagattatt agcagcataa tatggggtga ggataatggc cgctccgtgc
    13261 ggccttttga ttaaataaac cccagcagag aaaagaacac ataacctgca gcaatacaaa
    13321 taaccggcag gatatacagc gagaaaaact gcaggaaaat agtatggtgc ggcacaacaa
    13381 tacgtgactc aatttgttca cgcgttagcc cctcttcccc tttggctttt tccagaatga
    13441 gttgatcttc aacaccttcc cgcaagaagc gcgcctggcg actcatccgg gcaccggaat
    13501 cctgcaacgc cagtccgaca aaaatcagga tgaaaatcac ccagaacata acgttcacgc
    13561 caccattaaa attgggcgtc ggggagttat accagaacag attcaaaaaa ggcgtattcg
    13621 cctgcatcat atcgatcatg acatgagtaa agtcgagcat gaccgcatta atgccttcct
    13681 ggttttcctg ccgggtattc ataaatttca gcaaagaaat cagtgtagag atcaatgcag
    13741 gtataaaaat cacccaaccc aaaatccttt tcaaaacagc aatgcgtcca gcttgttgat
    13801 acgtcatgag ttctccttga ttaagacgcg tcgtttcact tagtttacct gtagatatct
    13861 gttttcgccc attctttaaa ggcgatatga taggcgctta atcataagca cggcttaata
    13921 ctttacacat aatgctctaa aggagaggtt gtaatgtcaa ccccgcgtca gattcttgct
    13981 gcaatttttg atatggatgg attacttatc gactcagaac ctttatggga tcgagctgaa
    14041 ctggatgtga tggcaagcct gggggtggat atctcccgtc gtaacgagct gccggacacc
    14101 ttaggtttac gcatcgatat ggtggtcgat ctttggtacg cccggcaacc ctggaatggg
    14161 ccaagccgtc aggaagtagt agaacgggtt attgctcgtg ccatttcact ggttgaagag
    14221 acgcgtccat tattaccagg cgtgcgcgaa gctgttgcgt tatgcaaaga acaaggttta
    14281 ttagtgggac tggcctccgc gtcaccacta catatgctgg aaaaagtgtt gaccatgttt
    14341 gacttacgcg acagtttcga tgccctcgcc tcggctgaaa aactgcctta cagcaagccg
    14401 catccgcaag tatatctcga ctgcgcagca aaactgggcg ttgacccact gacctgcgtg
    14461 gcgctggaag actcggtaaa tggcatgatc gcttctaaag cagcccgcat gcgttccatc
    14521 gtcgttcctg cgccagaagc gcaaaatgat ccacgttttg tattagcaaa cgtcaaactc
    14581 tcatcgctga cagaactcac cgcaaaagac cttctcggtt aatgaccagg ggcagtgatc
    14641 acactgctcc tggttcttta tctgaatttg cattcaactg acggattaat cgtcaattta
    14701 agagaaagag ttacaccgtc accacttccg tgcactgtat aaaaatccta tactgtacgt
    14761 attgacagtt tagtgagttt tatcatgacg gcggaaggtc accttctctt ttctattgct
    14821 tgtgcggtat ttgccaaaaa tgccgagctg acgcccgtgc tggcacaggg tgactggtgg
    14881 catattgtcc cttccgcaat cctgacgtgt ttgttaccgg acatcgatca cccaaagtcg
    14941 tttcttgggc agcgattaaa gtggatatca aaaccgatcg cccgcgcttt tgggcatcgt
    15001 ggttttaccc acagtctgct ggcggtattt gcgctgctgg caacctttta ccttaaggtt
    15061 cctgaaagct ggttcattcc ggctgatgcg ctacaaggaa tggtgctggg ttatttgagc
    15121 cacatacttg ccgatatgct gacacccgcc ggtgttcccc tgctctggcc atgccgctgg
    15181 cgtttccgct tgcctatcct ggttccccaa aagggcaacc aactggaacg ttttatctgc
    15241 atggcattat ttgtctggtc ggtatggatg ccccattcat tacccgaaaa cagcgctgtt
    15301 cgttggtcat cgcaaatgat caatactttg cagatccagt ttcatcggct tattaagcat
    15361 caggttgaat actaaaaagg caaaaatcac ctttctggaa taagcaattc catttgaata
    15421 taagagccag ctcacagttc tgttaatctt gcgccaacac tatgactgct acgcagtgat
    15481 agaaataata agatcaggag aacggggatg aactttccat taattgcgaa catcgtggtg
    15541 ttcgttgtac tgctgtttgc gctggctcag acccgccata aacagtggag tctggcgaaa
    15601 aaagtgctgg tgggtctggt gatgggtgtg gtttttggcc tcgccctgca taccatttat
    15661 ggttctgaca gccaggtact taaagattct gtacagtggt ttaacattgt tggtaacggc
    15721 tatgttcaac tgctgcaaat gatcgttatg ccgttagtct tcgcctctat tctgagcgcg
    15781 gttgcccgtc tgcataacgc atctcagtta ggcaaaatca gttttctgac catcggtacg
    15841 cttttgttta ccacgctgat tgcggcgctg gtcggtgtgc tggtcaccaa cctgtttggt
    15901 ttgacggctg aaggtctggt tcagggtggt gcagaaactg cacgtctgaa cgccattgaa
    15961 agtaactatg ttggtaaagt ctctgacctg agcgttccgc agctggtctt gtcctttatc
    16021 ccgaaaaacc cgtttgccga tctgaccgga gccaatccga cgtcaattat cagcgtggta
    16081 atttttgccg cattcctcgg cgtagctgcg ctgaagctgc tgaaggatga tgcgccgaaa
    16141 ggtgaacgcg tcttaaccgc tatcgatacc ctgcaaagct gggtgatgaa actggttcgc
    16201 ctggtcatgc agctgacccc ttacggcgtt ctggcgctga tgaccaaagt ggttgcaggt
    16261 tctaacctgc aagacatcat caaactggga agtttcgttg tcgcgtccta cctcggtctt
    16321 ctgattatgt ttgcagtgca tggccttctg ctgggtatta atggcgtgag cccgctgaag
    16381 tacttccgta aggtatggcc agttctgacg tttgccttta ccagccgttc cagtgctgcg
    16441 tctatcccac tgaatgtgga agcacaaacg cgtcgtctgg gtgttcctga attcatcgcc
    16501 agtttcgccg cctctttcgg tgcaaccatt ggtcagaacg gctgtgccgg tttgtatccg
    16561 gcaatgctgg cggtgatggt tgcgcctacg gttggcatta acccgatgga cccgatgtgg
    16621 attgcgacgc tggtcggtat tgttaccgtt agttccgcag gcgttgccgg tgtcggtggt
    16681 ggtgcaactt tcgccgcact gattgtgctg cctgcgatgg gcctgcctgt aacgctggtg
    16741 gcattgttaa tctccgttga accgcttatc gacatggggc gtacggcgct aaacgttagt
    16801 ggctcgatga ccgctggcac gctgaccagc cagtggctga agcaaaccga taaagccatt
    16861 ctggatagcg aagacgacgc cgaactggca caccgttaat tcttatgctg gcaacggtcc
    16921 gttttgtata ggagccgttg ccttactttt taaatgttcc atcgcggcga tgcatactga
    16981 ctcatattta aacagatcgt cagcgaaaaa aatggctaac ttatcgatac attcctgact
    17041 caatgaatgt gcttgtagcg agaagcttaa aacctcatac ccaagacgtt ttctaagtga
    17101 actatctgaa atactcataa tctcttcaag cacaccactt cccattcccg gaagattcag
    17161 tggattaaaa agaagattga cattaagcat tgatattacc gaaggaaact ttaggagact
    17221 ctcatttttt tgaatcgcag caaaaatgct cccaacagga tgtatataat tttgttcata
    17281 gtatatcgtt cttaaatagt gaataaccaa cctgtttttt atattgatca aattggtcat
    17341 tttttctggt cggaattcac aggtaaaatc catcccaaca aaattctcat taatacctct
    17401 tatcctaatt tcactaccat aaagattatt aattatcact ctatatttaa cattgtcctg
    17461 cagatatgta tcaaaactaa gtgctggcgc tcctggcttt gaggcatcat agttaatgtt
    17521 agacatatag gcaatcatat aattgaagtt tgattctgct atttcatcgc aggcacattg
    17581 acccgttgat ataaagaaag tgagtgctgt catggtagac tgcagtcttt ctttaaaaga
    17641 gtcgaaggca aggtttttaa tatttaaatt accataatca ttctgtatta agtttgtaga
    17701 aaacatctcc cgcctcatat tgttaacaaa attattatct catttaaatc taagtcattt
    17761 acaatataag tttaagagcg acaccacagg atgaactatc aaaaatagct catcatgatt
    17821 agcaaaactt aaccatttta aaataaataa acaattaaag aaaaaagttc acttatttat
    17881 agcaatagat cgtcaaaggc agctttttgt tacaggtggt ttgaatgaat gtagcaatga
    17941 aatacagaat ttcaggccat gtaactcccg gcagaaccgg gaggtacgta atccttactc
    18001 agtcacttcc ccttcctggc ggatctgatt tgcccaacgc tgggcggatt caggcacagt
    18061 aaacgccggt gagcgcagaa atgactctcc catcagtaca aacgcaacat atttgccacg
    18121 tagcatccag acatcacgaa acgaatccat ctttatcgca tgttctggcg gcgcgggttc
    18181 cgtgcgtggg acatagctaa taatctggcg gttttgctgg cggagcggtt tcttcattac
    18241 tggcttcact aaacgcatat taaaaatcag aaaaactgta gtttagccga tctagccccc
    18301 atacgtcccg ctttgcgtgt atttcataac accgtttcca gaatagtctc cgaagcggga
    18361 tctggctgat ggtctatagt tagagggttt tttgaccaaa acagcggccc tttcagtaat
    18421 aaattaagga gacgagttca atgtcgcaac ataacgaaaa gaacccacat cagcaccagt
    18481 caccactaca cgattccagc gaagcgaaac cggggatgga ctcactggca cctgaggacg
    18541 gatctcatcg tccagcgact gaaccaacac cgccaggtgc acaacctacc gccccaggga
    18601 gcctgaaagc ccctgatacg cgtaacgaaa aacttaattc tctggaagac gtacgcaaag
    18661 gcagtgaaaa ttatgcgctg accactaatc agggcgtacg catcgccgac gatcaaaact
    18721 cactgcgtgc cggtaaccgt ggtccaacgc tgctggaaga ttttattctg cgcgagaaaa
    18781 tcactcactt tgaccatgag cgcatcccgg aacgtattgt tcatgcacgc ggatcagcag
    18841 ctcacggtta tttccagcca tataaaagct taagcgatat caccaaagcg gatttcctct
    18901 cagatccgaa caaaatcacc ccagtatttg ttaggttctc taccgttcag ggtggtgctg
    18961 gctctgccga taccgtgcgt gatatccgtg gctttgccac caagttctat accgaagagg
    19021 gtatttttga cctcgttggc aataacacgc caatcttctt tatccaggat gcgcataaat
    19081 tccccgattt tgttcatgcg gtaaaaccag aaccgcactg ggcaattcca caagggcaaa
    19141 gcgcccacga tactttctgg gattatgttt ctctgcaacc tgaaactctg cacaacgtga
    19201 tgtgggcgat gtcggatcgc gggatcccac gcagttaccg caccatggaa ggcttcggta
    19261 ttcacacctt ccgcctgatt aatgccgaag ggaaggcaac gttcgtacgt ttccactgga
    19321 aaccactggc aggtaaagcc tcactcgttt gggatgaagc acaaaaacta accggacgtg
    19381 acccggactt ccaccgccgc gagttgtggg aagccatcga agcaggcgat tttccggaat
    19441 acgaactggg cttccagttg attcctgagg aagatgaatt taagttcgac ttcgatcttc
    19501 tcgatccaac caaactcatc ccggaagaac tggtacccgt tcagcgtgtt ggcaaaatgg
    19561 tgctcaatcg caacccggat aacttctttg ccgaaaacga acaggcggct ttccatcctg
    19621 ggcatattgt ccccggtctg gatttcacca acgatccgct gttgcagggg cgtttgttct
    19681 cttataccga tacacaaatc agtcgtcttg gcggacccaa tttccatgag attccgatta
    19741 accgcccgac ctgcccttac cataatttcc agcgtgacgg catgcatcgt atggggatcg
    19801 acactaaccc ggcgaattac gaaccgaact cgatcaacga taactggccg cgcgaaacac
    19861 cgccggggcc gaaacgtggc ggttttgaat cataccagga acgcgtggaa ggcaataaag
    19921 ttcgcgagcg cagtccatcg tttggcgaat attattccca tccgcgtctg ttctggctaa
    19981 gtcagacgcc attcgagcag cgccatattg tcgatggttt cagttttgag ttaagcaaag
    20041 tagttcggcc gtatattcgt gagcgcgttg ttgaccagct ggcgcatatt gatctcactc
    20101 tggcccaggc ggtggcgaaa aatctcggta tcgaactgac tgacgaccag ctgaatatca
    20161 ccccgcctcc ggacgtcaac ggtctgaaaa aggatccatc cttaagtctg tacgccattc
    20221 ctgacggtga tgtaaaaggt cgcgtggtag cgattttgct taatgatgaa gtcagatcgg
    20281 cagaccttct ggccattctc aaggcgctga aggccaaagg cgttcatgcc aaactgctct
    20341 actcccgaat gggtgaagtg actgcggatg acggtacggt gttgcctata gctgctacct
    20401 ttgccggtgc gccttcgttg acggtcgatg cggttattgt cccttgcggc aatatcgcgg
    20461 atatcgctga caacggcgat gccaactact acctgatgga agcctacaaa caccttaaac
    20521 cgattgcgct ggcgggagac gcgcgtaagt ttaaagcaac aatcaaggtc tctgaccagg
    20581 gtgaggaagg gattgtggaa gctgacagcg ccgacggtag ttttatggat gaactgttaa
    20641 ctctgatggc agcacaccgc gtgtggtcac gcattcctaa gattgacaaa attccggcgt
    20701 aaatatggat aaggtcacgc cccctgggga gtgaccttac caccttactt acacatcaag
    20761 ataattaccc aaccgataac cacgctcagc aatcgcatat ttcaacgacg ctgaagtcag
    20821 cacatccagt tccgttaacc gagggaagca ataagcactc tggcgaatcg tattatcgat
    20881 aaacgccggg tggcacatca cttccagcga gcgctcgccg cgatgactgg agtcatcaag
    20941 cacttgcagg aacagtgtct cgctaatctc ttcaccataa aacgcgctac tgaatccctg
    21001 cgaactgcgc agattagccg gtaaatcacc atccttagaa agcgactgac ggtcgatgcg
    21061 caacgcaatc ccctcttcag ccgcaaacct ggcaacaatc gggaaaatct gcgggaacat
    21121 atgcacatga tgatggctat caagatgcgt aggtttgcgt ccaaatagct caatgaaacg
    21181 caaatactga ctggcgagct cctgagtaat ttcttccagc ggtaaagcat cttcttctgc
    21241 caactgccag atccattttc ccagcacgcc atcgcgggtt aaccccggca tagctgtcag
    21301 tggcttgccc atagtaagga caaagtgcat ccctatggcc aaactcggtt catcacgact
    21361 caactgcact gcatggtcaa tagcctgccc attcaccagt gccgtcgtcg acgtgacaat
    21421 cccattgcga caggcctcga taatgccgta gttctgacct ttgcttaagc caaaatcatc
    21481 ggcattaaca atcagtaagc gttccataat cagcctcggt taatgtgctt ttttaagctc
    21541 tgcgatgcag tcggcaaaat ttggcagcca tttctcgtgc gccagaatca tctcgcgtgc
    21601 cagcagctca gcatcgcgat cggaatgcac caacggacta aggtttagcg ccagtaacac
    21661 atcgttaaat tctccgctaa gtgcagcgtt gctggcagca atctcgaagc ctttaatggt
    21721 gtgaatcagc cccatcactt tatcatcgaa atgcgtaatg cgcggatgtg gcgtcgcgcc
    21781 atcgcgcccc agcgtacagg tcatttctac cgcccagtct gccggaatat tatcaatatg
    21841 cccatgatgc gggatattaa cgtaatgttc agcttgcttg tcgttgtaga tagcgttgat
    21901 cacttcgcac gctgcatcag agtaataagc cccaccgcgc tgttccagtt ctttcggctt
    21961 aactttcaac tccgggtttt tatacagctc aaaaagttgt ttctcgactt tctgtactac
    22021 ctgtgctcgt gcgccgcctt tgtagtattc gcccatttca atagccagca tctctttttg
    22081 cttgaagtaa taaagtaaat aagaacacgg caacagattc agagaacgaa ttaagccttc
    22141 gctaaatggc agatcgaaaa tatttttaac gccagatgct tttaactgcc cggatgccac
    22201 accatcaagc aattcggcaa agcgtgattt gccatttacc agcacatctt taatgaacac
    22261 catatggttg aggccgaaca gatcgataga taaatcatcg ctgtctttca gcatcagaac
    22321 atcgcgaata aacatcttca tgccgatcgg aatattacac acgccgataa agcgtttaaa
    22381 tccggtatga cgataaacgg cttcagtgac cattcccgcc gggttagtga agttaatcac
    22441 ccatgcattc ggacaaagtt cttcgacatc ttttacgatg tcaaaaatca ccggaatggt
    22501 acgcagacct ttaaacagac cgcccgcgcc gttagtttcc tgaccaagat aaccatgact
    22561 taatggaata cgttcatcca gttcgcgcgc cggtaattgg cctacgcgca gttgggtagt
    22621 aacgaaatca gcatctttca atgcttcgcg gcgatccagc gttttataaa gcttcatcgg
    22681 gacgccagcg ttatcaatca tccgttggca gagatcaaaa ataatatcca gtttagcttt
    22741 accaccttcg acatccacca gccataattc gctgaccggc aattcgtgat aacgcttaat
    22801 aaatccttcc agtaactccg gggtatagct gctcccgcca ccaatagtga cgacttttaa
    22861 tttctggctc ataatttctc ccttcagtac agaatactga tatctggcat atctgccccc
    22921 ccggacataa ataatccagc aacaggacag atatgtgaat tgtcaggtat aacgacttac
    22981 tgattaaatt cagtcaattt cttacgatag ctcttaggcg taaaggacgt taatttctta
    23041 aacgttttaa taaacaaact cgggctacta taaccggcct caaacgcaat atccgtcact
    23101 gaatagttgg tcatttccag ttgttttttg gcaaaattaa tacggatttc attaataatc
    23161 tgcattggcg ttttgccata atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg
    23221 gctgacaacg tcaccatatt ctccagtgcc gactcactaa actgctcttt atcatgcatc
    23281 ttttctaccg tacttttcag ccactgcggt acatcatcaa tcacctgttc ttcgcggtaa
    23341 tgacgtaaac ggttaatgac ataaaaagta accatctcaa caaactcttc cagccctgtt
    23401 tcgcggaaat tcaatgaaga aatcactgtt tccacatagg tgagaaaagc gttattggtc
    23461 cggtatacct gcgaagcgac aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa
    23521 aagcgtttac tgatcccaac attcaatatg cgcgtggcac caaactcata aaaactttga
    23581 tggtgcgaac ctaacggaat aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg
    23641 ttaatctcct ggaaataacg cccggttaat accagagtaa attcataata gtcgtgctga
    23701 tgcagtccgc tgatactctc agttttgtta tagataaaca catggaagtt tttgccatta
    23761 aacaactgct gttctcgggc agtggcaatt tccggcgcgt taatcactgg ctgcatcatc
    23821 gactccttat gccttcagtt tttcatgaag ctcaattaat tctgtaatca gttcacgcgc
    23881 aagcatggac gtcattaaat gatcctgagc gtggaccagc accagactca ctttcatctt
    23941 accttcgccc gcatcgcctt caatcagttt cgtctgtacc agatgcgctt cattcaatgc
    24001 tatccgtgac tgatccatca tggcttttgc tgcggcaaaa tcgccctgct tcgcctgttt
    24061 cagtgccgca tacgccaggc tgcgcgcttg tccggagttg atgataagcc ccatcaccac
    24121 ttcttccagc tcttcagctt ccgtttgcgt atcgggaata ttatcgagat ccatcataca
    24181 tcgttcctct ctattcttac cggcacgatt acccgtaccg gcatcgatta aaatttcagg
    24241 gcgttagcga tatcttcttc gctctcttct ttatcaatcg cgttttgtgc tttgttagcg
    24301 accacaacaa agggcagata aattaacgtt gcgataccaa ggttgaagag tgcgaccagc
    24361 aatgcggcga cgctaccgtt ggtgttaaag aaggctccca gaccggttgg catggtccac
    24421 ggtgcaatat tggtcaccag aggaataatg cccatgtagt acgctgccag ggtgattgcc
    24481 gccagaatcg gttgtaccag tacaaacggg ataaacatca ccgggttcat gataattggc
    24541 agaccaaaca gaatcggttc gttaatctgg aagatgccgg acggcagcgc cagttttgcc
    24601 acctgacgat aatcagcacg gcgagaagcg ataaagatag ccaggatcag acctaaagtc
    24661 gcaccactgc caccgaggaa aataaaggag tccagcatcg gcttggccca gatatggaag
    24721 gtcttaccgg ctgccagcgc cgcttcgacg gaaccatatt gctgataggt cgcgatattt
    24781 tccagcgccc aaggcgtcat aatgccgttg tccagtgcgg tcagcgccag cgcaccatga
    24841 ataccgaaga accagagcag tggaacaaag atcacatagg cccagcccac cacgctaccc
    24901 aacgatgcca gtggggttga gatggtatcc ataatgatct gatggaagtt ggtgccccag
    24961 gtattcaacg cccaggcaat aatccccatc acggaaagaa taataaagcc gggaattaaa
    25021 gccgagaagg aacgcgatac tgaagcaggt acgctatcgg gcaatttaat gacccaattg
    25081 cggcggacaa taaaggtaaa catttctgcc accaccaggc caataataat cccggagatg
    25141 atattcgccc cacctaacca gtttgcgcca accgcatagg cctcaccgac actgtatggg
    25201 gtgacggtca taaatgctgc aacggataac aacccagccg ccagcgcatc gactttacgc
    25261 tcttctgcca gcgccatgcc aataaagaac ggtgccatta aagacattat ccctaatgtt
    25321 ccgttatata cgttgccgcc aatacctttc agaccattaa gtgtttcaat ggttgaggca
    25381 tccaggcgaa tacctaagga ataaaaaaac gacccctccc caaagcttag aaaaacgttg
    25441 ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat aaagccattt
    25501 ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag gagtaccttt
    25561 tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca atttaaatat
    25621 tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa ttaatttgct
    25681 gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca
    25741 taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg gatttcgggc
    25801 aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc tttttcacca
    25861 gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc tgcctgtgcg
    25921 cgcatttttg ataccagtaa agaggtagac atgcccgcag aacaaaacag ataaatgtgt
    25981 ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac tccgcaagcc
    26041 ttaacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt ttgtggctga
    26101 aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa attgtctctc
    26161 cttgacctgc ggttatgacc ctcttcacat ttcgggcaaa taattcgcga agataattaa
    26221 gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgtaactc aagctttcag
    26281 gcgaagtgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg tctgataacc
    26341 ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg ctttaccatc
    26401 acgttgaccc aggatgtagg tctggcaagt accacgagca tggatcatgc tcacttcaga
    26461 cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt tgacgtcttt
    26521 caccacaggc tgtacaaact ggtctttggt acgatcataa gccgtacaac ccgccagcat
    26581 ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat tccgtcctct
    26641 tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa gcccgtgaac
    26701 gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt cagcccgctg
    26761 tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt ttctgtctgg
    26821 aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa accgcagatt
    26881 aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct gcaaacttat
    26941 ccgttcatta aatcactggt gctagggatc agcggcggtc aggactccac gcttgccgga
    27001 aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctggcaacga atcactgcaa
    27061 tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg ccaggatgcc
    27121 attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc ggtattggcc
    27181 agcgaacagg cgctgcgcga agcgggcatt gaactgagcg attttgttcg tggcaatgaa
    27241 aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag cggtgtcgtg
    27301 gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata tggtgacggc
    27361 ggtacggaca ttaatccact gtatcgtctc aacaaacgtc agggtaaaca gttactggca
    27421 gcattaggtt gcccggaaca cctttataag aaagccccaa cggccgatct ggaagatgat
    27481 cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat cgacgactat
    27541 ctggaaggga aaaacgtacc tgaacaggtc gccagaacaa tagagaactg gtatctgaaa
    27601 accgaacata aacgccgtcc gccaattacc gttttcgatg atttctggaa aaagtaataa
    27661 tttgcacata ttggtttgtg cgaaaaagag tactttgttc acgccggatg cggcgtgaac
    27721 gccttattcg ccccacaaaa ctatgcaaat tcaatatatt gcatgagtcg aataggcctg
    27781 acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt ttttaatcgc
    27841 tccccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg tacggcgttt
    27901 aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg aacatttacg
    27961 ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga
    28021 caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc tttctcattt
    28081 acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct ggatctgcac
    28141 cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac aacagccgct
    28201 gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg
    28261 cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc tgtttggcct
    28321 gtttgccaat aggcgcgcag ctttgcaagc attgcagacc atcgccgatg aacaaaaact
    28381 ttgttatggc ctgttgggac tggaaccgtt tagccgcggt cgttcatgtt ttcgttctgc
    28441 attaaaacgt tgcgccgggg cgtgttgcgg taaagagagc catgacgatc atgcgctacg
    28501 cttgcgccag gctctggaac gtttgcgagt agtttgttgg ccgtggaatg gtgcggtggc
    28561 gctgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact ggctgtggct
    28621 gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg ccgggtttga
    28681 tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaattatg aaattactga
    28741 acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag tttgccttgc
    28801 ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg
    28861 agcagtttgt cgcccaattg ccacgccccg ttaatgacaa acactacccc gccgcgtgag
    28921 ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac atccaggcgt
    28981 gtcatgatgt tgaaatccat cgacatctgc cctgccgtca gtttcgcttt aaccacctgg
    29041 tctgccgcaa aggcaaaagg ctgtaacggc tttagggtat ggttaaagcg gtctgcgctt
    29101 tcaaggaaca tctcgccgcc ttccagcaac gtcactatcc tttccatacc gggaaacaaa
    29161 gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa atcacgtttt
    29221 gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca cagattcacc
    29281 gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt ttcattgtta
    29341 tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta gtggcgatca
    29401 ggcttttttt gcatctgttt gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt
    29461 actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag attattcagc
    29521 agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct caaaattagc
    29581 attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct gggtttccat
    29641 gtgcgccagc atattagctt tgcgctgttc ttccattttt gcgatctgcg cttcagcttt
    29701 tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcgacgtt cttccagcgg
    29761 cggacgtttc atctggtcac gctggccttt catgatttcg cggatctgct gtttctgcgc
    29821 gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact tgcctttgtg
    29881 gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg catgggccag
    29941 gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt tacgcatatt
    30001 ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc gtgttgacga
    30061 gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca atgaaagtgt
    30121 aaaaaacact tttgcgccaa ttatggagaa aaaagaaaat ttgatggaga gtgatgaggg
    30181 aatattacaa cacgatgatt ttgcagagat tatgaagaac tataccggat gactggtgat
    30241 aaataaagca aataaccagg attaatctgt attaatttat aagaaagtaa cttaataccc
    30301 gcagaatgat ttctgcgggt aagtattagc ttattttttc gagcattaat cccgcgcgta
    30361 atcccaacgc taccaaagga ttagggaata acacatactc tacatcatgg gttacggtaa
    30421 aacgttcctc cccgtcctgc gccagcaatg ttcctttctc aaacggcata aaattcagcg
    30481 tgtcacttgc catatgcatt tcgaaggacg gcgagtggcg agtaatttgc gaaaccaccc
    30541 gataacggag cggcggcgtt ctcacgatac cgacactctc accagatagc agcgcagcaa
    30601 ttgcgctggc agtgactgca aactggcgaa gatcgttttg tccaaagggc aacgctttgc
    30661 cgagttccag cgtacaggcc agcgcgccaa aatgtctggc gctgaaatgg gtaaacgtac
    30721 caccaggctc ctgatggaac accagtgcct ccagacccgc cgcacccagc cacgtcagaa
    30781 atttctcgtc ccagggaatg tcgcgttgcg gtaatacacc gaaccgcgga tgcaaggagc
    30841 cacgaattgc ggtatggaga tcaaggtgcc agcgcacaga ttctttgccc tggtcataaa
    30901 aatcttccag gcactgttcc agttcgcgcg cgcgacaggt ttccccgctt tcagcaaata
    30961 gctgccaacg accgccaaac attcggttca tatcgctatg gcaataacgt ttcccttgct
    31021 tcagcgcagg aggattcccg aggatcacca gcaaccgcca acgtaacggg atctcgccgt
    31081 gagatatcgc gccaagcaac gcgtccagca tctccatagg tgccgtctca ttaccgtgta
    31141 ttcccgctga aatcaccagt gcgccttgcg gtggcgttaa tggcgtcagt tccagcacac
    31201 catcgcccag ccagcgccag cgaacgccgt tgatttctcg ctcggtgata accggttttt
    31261 tacccgttaa ggtcagagca agaaaattat ccattgcccc ctccctcgcg ctggaacgga
    31321 taaaccgaac cgagattcag taattggctc aatacatcca gtgcttcccg cccttcacgc
    31381 agcaattgcg ggtcggccag atcggcagca gtaagacgat cgcggtagta acgatccacc
    31441 cagtcattga gcacattaaa cagcgtatcg ttcatcatca ccgccggatt caccgcccgg
    31501 cgttcttctt ctgtcaatac cacgcgcaac cgcaggcacg ccgggcctcc gccattcgcc
    31561 atgctttcac gtaaatcaaa gacttttagt tcgctaatcg ggttgtcagc ggcaaggagt
    31621 tcattgagat acccccatac tccggcgtgt tcccgacact cctgaggcag caccaacatc
    31681 atggaaccat catcgcggct cagcagttgg ctgttaaaca gatacgtaga caccgcatcc
    31741 gacacggaaa cctgtgccgc cggaacttct atcgctataa aactattgac ccgcgaacgc
    31801 aggtttgcca gtaactgtga ctggcgagcg aacgcctgtt gatggcaaaa cagcacctgg
    31861 cggttgctca cggcaatcac gtcattatga aaaacgccca ggtcgataac gtccgggttt
    31921 tgctgggcga aaatcacctg ttggggattc acctgattca accttgccac cgcctcgctg
    31981 gcttcgcgag tctgccgcgc cggataacgg gaaggccggg tatcattgcc ttcttctcgc
    32041 ccgtagacaa aaagctgcat acctggttcg ccataatgac cgccgagacg attgtggttt
    32101 gccgctccct catcaccgag caaggctacc tgcggcaacg ccgaatggac ggtgaatttt
    32161 tcttcatcgt taaaaatcgc ttttaacagc gattcggtga caggggcttc cagcgaacgg
    32221 tgaaatttat tgttcaggtt ggcaacggtg aggtgcactt tgccatccag cgtatcggca
    32281 gatggcgcga tcgttgccgc attggctacc cacattggcg aagcggagct gacgctggaa
    32341 agccagtgcg gtgcctggcg tgcaaccttt tccagtacct gctcatcgct accactgaat
    32401 cccaactgac gcagcaccgg aataaacgga cgctcgtgcg gcgggatcac ggcctggggg
    32461 aatcccgcat cggcaagggt tttcattttc agtaagccct gtttcgccgc cagtcgcggg
    32521 ttagagacct gaaaacggtg acgggtagag gcttcattac caaacgacag gcccgcgtaa
    32581 tgatgcgtca gccctaccag cccgtcgaaa ttgacttccc agtcgttcat cgcaccacct
    32641 cttcggaaaa atccagcccg gggttaagtg tggcgggcaa tgttaacgag tccgactcca
    32701 ggctcgccat cggccaggca caataatctg cggcatacca ggcgctgggg cgatggttac
    32761 cggaagcgcc aatgccgccg aatggcgcgg tactggcagc gccggtaagc ggtttgttcc
    32821 agttaacaat ccccgcccgt gcctcaagca gcagttgatc gaatttttcc cgctcggggg
    32881 aaaccagacc gcaagagagt ccgaagcggg tgttgttcgc catccgaacc gcctcatcaa
    32941 aggtgtcata acgccagacg cgcaatagcg gaccgaacac ctcttcatct ggtacgccag
    33001 caacgcccgt catttcaatg atccccggcg tcagcaacga tgtccctgct tgtaataagc
    33061 gcggcgcaag cagggggcgt ccacccatcg cttccagttg ctgccaggcc gtcaccacct
    33121 gctgcgcggc ctgatctgat atcagcccgc caataaacgg ctgcggttcg tcatcccagt
    33181 tgcccggcgt taatcgctgg ctgacggcaa ccagacgagc aagaaacgca tcgccctgcg
    33241 ccccgctttt cagcaataaa cggcgggcgc aggtgcagcg ttgcccggct gtgacaaatg
    33301 ccgactgaat ggtcagatgg acagcggcat cgatatccgc cacctcatcg ataatcagcg
    33361 ggttattgcc gcccatttcg agggcgagaa ttttctccgg ctgaccggaa agctgacgat
    33421 gcaactggta gccagtattg gcgctaccgg taaacagcaa gccgtcgaga tcctccagcg
    33481 cactcagcgc ctgacccgtt tcacgcccgc cctgcaccag gttcagcacg cccggcggca
    33541 agccagcctg ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg
    33601 gtttaaagat aatggtgtta cctgccagca atgccggaac gatatgtccg ttcggcaaat
    33661 gaccagggaa attatacggc ccaaacaccg ccagcacgcc gtgcgggcga tggcgcaggc
    33721 tcgccgcgcc gtccggcatt tcgctacgct gctcgccggt acgaacatga tacgccttaa
    33781 ttgatatcgc gattttattg atcatcgccg tcacttcggt tgccgcttcc cagcgcggct
    33841 tacccgtttc tctggcaata atcgcggtta attcggcttt attgctttcc agcaatccgg
    33901 caaagcgttc aactcttacc tgacgatcgc caaaggagag acgcgcccag cgcggaaacg
    33961 ccgcacgggc tgcccgacaa gcctgcccga cctgagcggc atcggcatca ttgccttgcc
    34021 ataacacctc gcccgatacc ggattacgct tcacacgcaa tgcgccctgg cccgttaccc
    34081 aatcaccgtt aatccataaa gtcatgctgt tttctcctct gcgcacaggc gcaccagacg
    34141 aacgcgatcc cctgcgtggc atttgagggc atccagttgt gcggcggtta aaatcaaacg
    34201 ctcggttgcc ggatcggtac gcaccagcac cacgcggaaa tggtgataat tttcattggc
    34261 gaccaggcag gccggaaaat cgccctgcgc aggctgccct tctgccactt ccaccagccg
    34321 acttttacgg atggcgcgca cgcggtcgat gtcacactca agcgtcggcc caccgtcgaa
    34381 gatgtcgata taattacggt agcgaaaacc ttctttctcc agtaccgcac gggcaggcgc
    34441 ggtttgcgga tgtacctgac cgatgacgtc ctgggcttcc tgagataaaa agtgggtata
    34501 gatcggatgt ttcggcatca gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa
    34561 atcggcgcgg ctaaaatcca tcgaaaagaa gcgcttaccg aggctttgcc agaacggtga
    34621 atagccatgt tcgtcaatca ccccgcgcat ttcggcaacc actttgtcat taaacttgtc
    34681 gcgaaaagcc gccataaaca taaagcgcga tttcgacagc aaaaagccgt tgccctcttt
    34741 gcgccactcc gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt gatcgttact
    34801 gagaaacagc gtcggcaacg cgttatagac attcagctct ttcgaagcgt gaaccagcgt
    34861 gccgacgcga tagttgtacc agggatcgtt cagcccaact gccacctcaa tggcgcaaat
    34921 ccccgccact gtgcctgtct cgctatcttc cagcacgaac acatagccct gctcactttt
    34981 gggcagttcg ccttgccagg ttttgattgc cctttcgata cgcgccgaaa gcgtggcttc
    35041 attggcggga agcgacgtca ggccgccgcc cgttttgctg gcaagctgca tcagcgccga
    35101 gacatctgaa cgctcaacgg gacggatgac catcatgaag aacctcggct aacaaagtgt
    35161 tcgcaagcag ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct aacattgagc
    35221 gcaggcgcaa aacgcaccac gttgccaccc gcaatcagta ccatcacgcc tgctttcgcc
    35281 gcttcctgag agatctgttt cgcttgcccg gcgtaatcgg cattcaacac gcaaccaatc
    35341 aacaaaccta agccgcgaac ttcactgaac aaaccgtagc ggtgattaat ggtattaaga
    35401 cgctcaacaa accagtcgtg acgctgttta acgccattaa gcatctctgg cgtgttgatg
    35461 agatccagca ctttgcctgc caccgccgag gccagcggat tgccgccata ggtggtgcca
    35521 tgagtgccaa cagtcatcac gctggcgcac tcttcggttg ccaacaacgc accgaccggg
    35581 aaaccgccgc ccagcgcttt ggcggtagtt aacagatcag gcgtcacgcc gtagtgcata
    35641 taggcataca gttccccggt gcgcccgacg ccggtttgta cttcatcaaa aatcaacagc
    35701 gcattgtggc ggtcacacaa ttcacgcaga ccttgtaaaa atgcgttgct ggctggcacc
    35761 acaccgcctt ccccctggat gggttcgaca atcaccgcac aggtagagtc gtcaatcagc
    35821 gcgctggcag agttaatatc gttatatgca gcatgacgaa tatccggcgg cagtggcgca
    35881 aaatcctgtg aataggcggg ctgcccaccc gcactgacag taaacagcgt gcgaccatga
    35941 aacgcatttt tgaacgccac gataccgctc ttatggctgc cgtagcggtc gtgagcgaat
    36001 ttacgcgcca gttttagcgc cgcttcgttg gcttccgcac cggagttaca aaagaagacg
    36061 cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtc gcagtaccgg ctcgttggtg
    36121 taaccgttgc cggtatgcca gaacttactc gcctgttcgt tcagcgcctc acgcagttcc
    36181 gggtgcgcat ggcccagcgc gttcaccgca atgccacccg cgaagtcgat atactctttc
    36241 ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg gtataaacgg tgccggagcg
    36301 taaacaggta tcatccattc atcaaagttt tcacgcgtaa ttggctgaga catagcgacc
    36361 tctacagtaa atacttcgtt atttatatgt taataataag taatgtttgc gctgtaaatg
    36421 tagattgcag ggttcgtgcc agccagtgat aaaagtgcat aaaccgcggt ggctaactgg
    36481 aaatcaagga gttataacca aaccatatgc atttaaagtg catataaagt gaatacgttt
    36541 gcgatatggg tgaataaaaa gaataaaaaa cgcaatgtta tgcagaagta aaatataatt
    36601 ctggaattgt gatcattgac gaaatttact ggaaattact gcgccattct gacgcagcgc
    36661 gcaccaaaag cgggcatttt ttgcgccatc gttgacatca ttaacaacca tcgatcaaat
    36721 cacttaacaa caggcggtac gcaaagcgaa attctgctac catccacgca ctctttatct
    36781 gaataaatgg cagcgactat gaaatttgtc tcttttaata tcaacggcct gcgcgccaga
    36841 cctcaccagc ttgaagccat cgtcgaaaag caccaaccgg atgtgattgg cctgcaggag
    36901 acaaaagttc atgacgatat gtttccgctc aaagaggtgg cgaagctcgg ctacaacgtg
    36961 ttttatcacg ggcagaaagg ccattatggc gtggcgctgc tgaccaaaga gacgccgatt
    37021 gccgtgcgtc gcggctttcc cggtgacgac gaagaggcgc aacggcggat tattatggcg
    37081 gaaatcccct cgccgctggg caatgtcacc gtgatcaacg gttacttccc gcagggtgaa
    37141 agccgcgacc atccgataaa attcccggca aaggcgcagt tttatcagaa tctgcaaaac
    37201 tatctggaaa ccgaactcaa gcgtgaaaat ccggtgctga ttatgggcga tatgaatatc
    37261 agtcctggcg acctggatat tggcattggc gaagagaacc gtaagcgctg gctgcgtacc
    37321 ggaaaatgtt ctttccttcc ggaggaacgc gaatggatgg agagactgat gagctgggga
    37381 ttggttgata ccttccgcca tgcgaatccg caaacagcag atcgtttctc atggtttgat
    37441 taccgctcaa aaggttttga cgataaccgt ggtctgcgca tcgacctgct tctcgccagc
    37501 caaccgctgg cagaatgttg cgtagaaacc ggcatcgact atgaaattcg cagcatggaa
    37561 aaaccgtccg atcacgcccc cgtctgggcg accttccgcc gctaatttag cagctctcct
    37621 ggctcaaact gggtcaggag aattaacctt gagaaaaatc aacaaactgt cagtaatgat
    37681 ttgttgcctg ccgccctttg ttataccgtc actgcgtttt tagttgtctg accacttctc
    37741 tattatcaag tttgatatag gaaactccac aatgaacgct gagcgtaaat ttctttttgc
    37801 ctgtcttatt tttgcgctgc tcatttacgc tatccacgct ttcggtttat tcgagctgct
    37861 caccgattta cctcacttac agacactcat tcgccagagc ggattgttcg gttatagcct
    37921 ctatattctg ttattcatca ttgccaccct ctttctgtta ccaggaagca tactggtgat
    37981 cgctggaggg atagtttttg gcccgctctt agggacacta ctatcattaa ttgccgccac
    38041 gctggcctcc tcatgctcat tcctgctggc gcgctggctg ggacgtgatt tactgctgaa
    38101 atacgttggc catagccata ccttccaggc cattgataaa ggcattgcgc gtaacggtat
    38161 tgattttctt attctgaccc gcttaatccc gttgtttcct tacaatattc aaaattacgc
    38221 ttacggatta accacaatca ccttctggcc ttataccctt atttcggcac tcacaaccct
    38281 gcccggtatt gttatttata ccgtgatggc aagcgatctc gccaatgaag gcattacgct
    38341 gcgctttata ttacaactct gtctggcggg catggcgctg tttattctcg tccagctcgc
    38401 caaactctat gcccgacaca aacatgtgga tctgtctgct tctcgccgca gcccacttac
    38461 tcaccccaaa aatgaaggat agaacgatgt tgcaacatta ttcagtgtca tggaaaaaag
    38521 gactgactgc actctgctta ctggctgttg ccgggcttag cggctgcgat caaaaagaga
    38581 atgcggcggc aaaagtggaa tacgacgaac tttcgaacag ccaaccgctg cgtgtcgatg
    38641 ctaataacca tacggtaacc atgctggtgc aaattaatgg tcgtttcctc accgacgaca
    38701 ctcgtcacgg tattgtgttt aaagatggct ccaacggaca taaatcgctg tttatgggtt
    38761 atgcgacccc gaaagcattt tatgaagccc tgaaagaggc aggtggtaca ccgggcgaaa
    38821 acatgacgat ggataataaa gaaacgactc atgtcacagg cagcaaactg gatatttcgg
    38881 tcaactggca aggggcggca aaagcgtatt ccttcgatga agtgattgtt gacagtaatg
    38941 gcaagaaact ggacatgcgc tttggcggta atttaacggc agcagaagag aagaaaacgg
    39001 gctgcctggt gtgtctggat agctgcccgg tcggcatcgt cagcaatgca acatacactt
    39061 atggtgcggt tgaaaaacga ggtgaagtta aattcaaagg caatgcctcg gttctcccgg
    39121 cggataacac gctggcaacg gttaccttta aaatcaccga ataaagccag gataaaggat
    39181 gatgaagatg caatcgcgaa aaatctggta ctaccgtata accctcatca tcctgttgtt
    39241 cgctatgctg ctggcatggg cgctgcttcc cggcgtccat gagtttatca atcgcagcgt
    39301 tgcggcgttt gccgccgtgg accaacaggg tatagaacgc tttattcagt cttacggcgc
    39361 actggcggca gttgtctcgt tcttgttgat gattttacag gccattgctg caccgctacc
    39421 tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc gcgttctggg gcggcttgct
    39481 gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt tttatcgcca gagtgatggg
    39541 ccgcgaagtg gtggaaaagt taacgggtaa aacggtactc aatagtatgg acggcttttt
    39601 tacccgctat ggtaaacaca ccattctggt atgccgatta ttgccttttg tgccttttga
    39661 tcccgtaagc tatgctgccg gtttgacttc aatacgtttt cgctcgtttt ttatcgccac
    39721 cgggcttggt cagttaccag cgactattgt ttattcctgg gcgggcagca tgttaacagg
    39781 cggtaccttc tggtttgtca ccggactgtt tattctgttt gccctgaccg tggtgatttt
    39841 tatggcgaag aaaatatggc ttgagcgcca gaagaggaat gcctgatggg tttaccgccg
    39901 cttagcaaaa ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg ccattacggc
    39961 gaggtgctaa gcccgattcg ctggtggggg cggatcccgt ttatctttta tctggtgtcg
    40021 atgtttgtcg gctggctgga gcgcaaacgc tcaccgctgg atccggtagt gcgatcgctt
    40081 gtcagcgcgc gcattgcgca aatgtgtctg tgtgagtttt gcgtggatat cactagtatg
    40141 aaagtcgccg agcgcactgg cagcaccgat aaactgctgg cagtggctga ctggcggcaa
    40201 agcccgctct ttagcgatga agaacggctg gcgctggagt acgccgaagc cgcaagcgtc
    40261 acaccgccaa cagtcgatga tgccctgcgt acccgactgg ctgcgcattt tgacgctcag
    40321 gcgctcaccg aactgacggc attgatcggc ctgcaaaacc tgtcggctcg ttttaattct
    40381 gccctggcga tccccgctca ggggctgtgc cgtattcctg aaaaacgttc ttaaggagag
    40441 atgatgcgcc attgtgggtg gttgctggga ttgttatcgc tgttttcttt ggcaacacat
    40501 gccagtgact ggcaggaaat caaaaacgac gccaaagggc aaaccgtctg gtttaacgcc
    40561 tggggcggcg ataccgcaat taaccgctat ctcgactggg ttagcggcga gatgaaaacc
    40621 cattacgcta taaacctgaa gattgttcgt ctcgcggatg ccgcagacgc ggtgaagcgc
    40681 attcagaccg aagctgctgc cggacgcaaa acaggcggtt cggtggatct gctctgggtg
    40741 aacggcgaaa acttccgcac cttaaaagag gccaatttat tacaaacggg ctgggtggag
    40801 actctgccca actggcgcta tgtcgacaca cagctgccgg tgcgggaaga tttttcagtg
    40861 ccaacgcaag gtgcggaatc gccctggggc ggcgcgcaat tgacgtttat cgcccgccgc
    40921 gatgttacgc cacagtcacc acaaacgccg caagccttac tggagtttgc taaagccaat
    40981 cccggtacgg ttacctatcc gcgcccaccg gactttaccg gcacggcgtt tcttgaacag
    41041 ttgctgatta tgctgacgcc cgatcccgcc gcattaaaag aagcgctgga cgatgcgact
    41101 ttcgcccgtg tcactgctcc cttgtggcaa tatcttgatg cgctgcatcc gtatttgtgg
    41161 cgcgaaggaa aggatttccc gccttcgccc gcgcggatgg atgctctgct gaaagccggc
    41221 acattgcgcc tgtcgctgac ctttaatcct gctcatgcgc agcaaaaaat cgccagcggc
    41281 gatttacccg ccagcagtta cagctttggc tttagcgagg gaatgattgg caacgtgcat
    41341 ttcgtcacca ttcccgccaa cgcgaatgcc agtgctgcgg cgaaggtagt tgccaatttc
    41401 ctgctctcac ccgatgcaca actgcgtaaa gcagaccctg ctgtctgggg cgatccttcg
    41461 gttctcgatc cgcaaaaact gcctgacggg cagcgcgaaa tattgcaatc aagaatgccg
    41521 caggatctgc cgccggtact ggctgaaccg cacgcaggtt gggtaaatgc gctggaacaa
    41581 gaatggctac accgttacgg tacgcattaa tctttttgct gtgggcgatg gtggcggtga
    41641 tttatgcacc gctgatcccg gcagctctca cgctgatatc gcctgctttg tcgttgacac
    41701 actggcagac gttatttgcc gatccacagt taccgcaggc attactggca acgctggtgt
    41761 cgacaaccat cgcggcggtc ggggcgttgt tgattgccct gctggtgatt gtgacgctgt
    41821 ggccagggcc gaaatggcag cgtatgtgcg cccgtctgcc gtggctgctc gccattcccc
    41881 atgtggcttt tgccaccagc gcccttctgc tctttgctga cggagggctg ctttatgact
    41941 atttcccgta tttcactccg ccaatggatc gatttggcat cggtctgggc ctcacccttg
    42001 cagtgaaaga aagcgcattt ctgctgtgga tcttagcggc agtattgagc gaaaaacggc
    42061 tgttgcagca agttattgta ctggattcgc tgggctacag ccgctggcaa tgcctgaact
    42121 ggctgttgtt gccctccgtc gcacctgcac tggcaatggc gatgctggcg attgtcgcct
    42181 ggtcgctgtc ggtcgtggat gtggcgatta tccttgggcc aggtaatccc ccgacgctgg
    42241 cagtaattag ctggcagtgg ttaactcagg gcgacgccga tcaacaaacg aaaggcgcac
    42301 tcgccagcct gctgctgatg ctgttactcg ccgcctacgt tttgctgggc tatctgctat
    42361 ggcgtagctg gcggcgtact attccccgcg tagatggcgt tcgtaagcct gctacgcctt
    42421 tattgccagg cattacgctg gcgagttttt tacccttaac cggtgtgctg tgtgtggttc
    42481 tgctggcgat cctcgcggat cagtcgacga tcaatagtga agcgctcatc aacagcctga
    42541 caatggggct ggtggcggca ttcatcgctt tgctcctgtt actgctgtgg ctggaatggg
    42601 ggccacagcg tcgccagttg tggctatggt tgcccatttt attacccgct ctgccgctgg
    42661 tggcgtgcca gtacacgctg gcgctatggc tgaacctgga tggaagctgg acggcggtgg
    42721 tctgggggca tctgctgtgg gtaatgccgt ggatgctgtt tatcctgcaa cccgcctggc
    42781 agcgcattga ttcacggtta attttgattg cgcaaacact gggctggtcg cgggccaaaa
    42841 tcttctttta cgtgaaatgt ccgcttatgc tacgccctac gctgattgcc ttcgcggtgg
    42901 gattttcagt cagtattgcg cagtatatgc cgacgctgtg gctgggcgcg gggcgttttc
    42961 cgacgctcac cactgaagcg gtggcattaa gcagcggcgg cagcaacggc attctcgccg
    43021 cccaggcttt atggcaactg ctattaccgc ttattatttt tgccctgacc gcgttagtcg
    43081 caaaatgggt aggttatgtc agacaaggac tccgctaatg ctctgcgtga aaaatgtttc
    43141 gctacgttta ccagaaagcc gcttgctgac aaacgttaac tttacggtag ataaaggtga
    43201 cattgtcacg ttaatggggc cgtctggctg tggaaaatct acactgtttt catggatgat
    43261 tggtgcgctg gccggacagt tttcttgtac aggtgagcta tggctcaatg agcaacggat
    43321 tgacatgcta cccaccgctc agcgtcagat tggcattctt ttccaggatg cactgttatt
    43381 tgaccagttc agcgtcgggc aaaatttact gctggctctg ccagcggcac tcaaaggtgt
    43441 tgcaaggcgc gatgcggtaa atgatgcgct ggagcgtgcg gggcttggcg gtatgttcca
    43501 tcaggatcct gccactttgt ctggcggtca gcgagcgcgc gttgctctgc ttcgcgctct
    43561 tctcgctcag ccaaaagcgt tgctactgga tgagccattc agccgtcttg atgtggctct
    43621 gcgcgataat tttcgccagt gggtgttcag cgaagttcgc gcccaggcga tccccgtcgt
    43681 tcaggtaacg cacgatctcc aggatgttcc tgctgatagt tctgttctgg atatggcgca
    43741 gtggtcagaa aattacaaca aactgcgata acgcaaagtt tttcccaatg cgtcagttca
    43801 gaatggcgca ctcaaaacta caatgtcggg attttcgatg aaacgtgttt ctcaaatgac
    43861 cgcgctggca atggctttag ggctggcttg cgcttcttcg tgggccgctg aactggcgaa
    43921 gcctcttaca cttgaccagc ttcaacaaca aaatggcaaa gcgatagata ctcgccccag
    43981 cgcgttttat aacggctggc cacaaacctt aaatggccct tctggtcatg aacctgccgc
    44041 cttaaacctc tctgctagct ggctcgacaa aatgagcacc gaacagctta acgcgtggat
    44101 caagcaacat aacctgaaag ccgatgctcc ggtggcgctg tacggtaatg acaaagatgt
    44161 cgacgccgtc aaaacgcgac tgcaaaaagc aggttttacg catatctcca ttctgagtga
    44221 cgcgctaagc gaaccttccc gtctgcaaaa actgccgcac tttgagcagc tggtttatcc
    44281 gcaatggctg catgacctgc aacaaggtaa agaggttacg gcgaaacctg ccggtgactg
    44341 gaaagtcatt gaagcggcct ggggcgctcc taagctttac cttatcagcc atattcccgg
    44401 cgctgactac atcgatacca acgaagtgga aagcgaaccg ctgtggaaca aagtttctga
    44461 tgaacaacta aaagcgatgt tggcaaaaca cggcattcgc catgacacca cggtcattct
    44521 gtacgggcgt gacgtatacg ctgcagcgcg tgtggcacaa attatgctct acgctggcgt
    44581 gaaagatgtc cgcctgctgg acggcggctg gcaaacctgg tccgacgcga gactgcccgt
    44641 tgagcgcgga acgccaccga aagtgaaagc ggaaccggat ttcggcgtga agatcccggc
    44701 acaaccgcaa ctgatgcttg atatggaaca ggcgcgtgga ctgctgcatc gccaggatgc
    44761 atcgctggtg agcattcgtt cgtggccaga atttatcggt acgaccagcg gttacagcta
    44821 tattaaacca aaaggtgaaa tagccggagc acgttgggga cacgctggta gcgactcgac
    44881 gcatatggaa gatttccata acccggatgg caccatgcgt agcgccgatg atattaccgc
    44941 tatgtggaaa gcatggaata tcaagccaga tcagcaagtt tcattctact gcggcaccgg
    45001 ctggcgcgca tccgaaacct ttatgtacgc acgagcaatg ggctggaata acgtctccgt
    45061 ttacgacggc ggctggtacg aatggagcag cgatccaaaa aatccggttg caaccggtga
    45121 acgcggcccg gacagcagca aataacattg caattactga cgctggagcg acttcagcgt
    45181 cagataccca ctccacaccc gcgtaaaggt cgtcatccag cacaacgcac cgaagatcca
    45241 cgcaaaccac gcaaaccatg cagggaataa acatcccagc acaaacagta agatcgtttc
    45301 agtgccttcg gttaatcccc ccagatagta aaacgactta tgtgcataac cggggttatc
    45361 aatctgatgt tttgctgcca gtgcagcaaa ggcgagaaaa ctgctgcccg tgccgataaa
    45421 cgcaaacagc agccagccgc ctgccagcgc gttttgctcc ggtgcggcaa ggataaaccc
    45481 aaaaggcacc agcgcgtaaa aaagaaaatc gagagaaata tcgagaaagc cgcccgcgtc
    45541 ggtaagccct ctgcgccgcg ccagcgcacc gtccagacca tcaagcagcc tgttcaacaa
    45601 aatggcgact agcgccgcca gataccagcc cagcgccaga aaaggcagtg ccagcacgcc
    45661 gatggcaaat cccactaatg ttaaaccatc tggcgtaatg cccggtttat caagaacccg
    45721 cacgcactga tgcaacaacg gtttaatccg gggatgaaga tggcggtcta gcacaggcac
    45781 tccttaaata taaagccttt ctgattgagc aacagtgcgg atattatggc atttttcgct
    45841 tatctgcccg tgtgtaattt atgaaaatga ttgaagttgt tgccgccatc atcgaacgtg
    45901 atggcaaaat tttactcgcg caacgccccg cccatagcga tcaggcggga ttatgggagt
    45961 ttgccggtgg taaagtcgag ccggatgaaa gccagcggca ggcgctggtg cgtgagttac
    46021 gcgaagaact gggcatcgaa gcagctgtgg gtgaatatgt tgccagccat cagcgagaag
    46081 tttcggggcg gattatccat cttcatgcct ggcacgtacc cgacttccac gggacgttac
    46141 aggcacatga acatcaggcg ctggtctggt gctcacctga agaggcgctg caatatccgc
    46201 tggcccctgc tgacattcca ctattagagg cgtttatggc tttacgcgcc gccagaccag
    46261 cggattagtg ctaagggttt tgtcatcacg ctggcattgc agcagtattc cttcggcttt
    46321 aattaccgcc ccttcagaat aattttgatc ctgataaacg cagcactgag tacagggctg
    46381 cgctgactgc ccgcctgaac tgaacacttc tggcggtacg tttacctcca cgtccggacg
    46441 ataatgcggg ttagccagtg caattaatgg aaatgctaat actacggcga acaatgctcg
    46501 actcacaggg aactccttaa cgttattgtc tctgctactg ataacggtag ccgggtggca
    46561 aaactttagc gtctgagtta tcgcatttgg ttatgagatt actctcgtta ttaatttgct
    46621 ttcctgggtc attttttctt gcttaccgtc acattcttga tggtatagtc gaaaactgca
    46681 aaagcacatg acataaacaa cataagcaca atcgtattaa tatataaggg ttttatatct
    46741 atggatcaga catattctct ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat
    46801 caaaccgagt tcgcgcaagc cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa
    46861 caaaatccaa aatatcgcca gatgtcatta ctggagcgtc tggttgaacc ggagcgcgtg
    46921 atccagtttc gcgtggtatg ggttgatgat cgcaaccagg tacaggtcaa ccgtgcatgg
    46981 cgtgtgcagt tcagctctgc catcggcccg tacaaaggcg gtatgcgctt ccatccgtca
    47041 gttaaccttt ccattctcaa attcctcggc ttcgaacaaa ccttcaaaaa tgccctgact
    47101 actctgccga tgggcggtgg taaaggcggc agcgatttcg atccgaaagg aaaaagcgaa
    47161 ggtgaagtga tgcgtttttg ccaggcgctg atgactgaac tgtatcgcca cctgggcgcg
    47221 gataccgacg ttccggcagg tgatatcggg gttggtggtc gtgaagtcgg ctttatggcg
    47281 gggatgatga aaaagctctc caacaatacc gcctgcgtct tcaccggtaa gggcctttca
    47341 tttggcggca gtcttattcg cccggaagct accggctacg gtctggttta tttcacagaa
    47401 gcaatgctaa aacgccacgg tatgggtttt gaaggaatgc gcgtttccgt ttctggctcc
    47461 ggcaacgtcg cccagtacgc tatcgaaaaa gcgatggaat ttggtgctcg tgtgatcact
    47521 gcgtcagact ccagtggcac tgtagttgat gaaagcggat tcacgaaaga gaaactggcg
    47581 cgtcttatcg aaatcaaagc cagccgcgat ggtcgagtgg cagattacgc caaagaattt
    47641 ggtctggtct atctcgaagg ccaacagccg tggtctgtac cagttgatat cgccctgcct
    47701 tgcgccaccc agaacgaact ggatgttgac gccgcgcatc agcttatcgc caatggcgtt
    47761 aaagccgtcg ccgaaggggc aaatatgccg accaccatcg aagcgactga actgttccag
    47821 caggcaggcg tactgtttgc accgggtaaa gcggctaatg ctggcggtgt agcaacatcg
    47881 ggcctggaaa tggcgcaaaa cgctgcgcgc ctgggctgga aagccgagaa agttgacgca
    47941 cgtttgcatc acatcatgct ggatatccac catgcctgtg ttgagcatgg tggtgaaggt
    48001 gagcaaacca actacgtgca gggcgcgaac attgccggtt ttgtgaaggt tgcggatgcg
    48061 atgttaagcc agggcgtgat ttaagacaat tgtgccggat acagcgcaaa cgccttatcc
    48121 ggcaataaaa atcttcagta tcagatggtt gaaagaacct ttatgttatc actccattgc
    48181 ccgcctcttc tgatggcggg ctttttttcg gcgctctctt gcgtggcgaa tcctttttct
    48241 tcccttctcc accgccggga gccattattc cccgaaactg ccttaccgac gtgcgctttg
    48301 cctgatcaat caactggtac aatgtcccca ccagcggctg cataaagtcc tgatagcgac
    48361 actgcttttc actgatttgc gtcagcaccg attcccagtg cgcggtcatg tccggtcgcg
    48421 tcgccatctc tggcagcgaa tggaataacg cttttccggc gtcggtggag tggatatagc
    48481 gccctttttt ggtcaggaag cctcgcttga acagcagttc aataatcccg gcgcgagtcg
    48541 cttctgtccc cagaccatcg gtcgcacgaa ggatcttttt cagatctttg tcctgcacaa
    48601 aacgtgctat cccggtcatc gctgaaagca gtgttgcatc ggtaaaatgg cgcggcggct
    48661 gggtttgccg ctctaccact tcaccttttt cacacagcaa ctcatcgcct ttcgccacca
    48721 caggcagcgg cgtgccgtcg ttttcttcat cgcgctcttt gctgcctaac agtgtgcgcc
    48781 agcctgcttc agcaagaaaa cgggctttag cgacaaattt gcctttggca atgtccagtt
    48841 cgataacaca cttgcggaac accgcatccg ggcagaactg catcaagtac tgacgggcaa
    48901 tcaggttata gaccttcgct tcgctctccg tcaggttgat cgcagaactc cgtgcggtcg
    48961 ggatgatggc gtggtgcgca tcgacctttt tgtcatccca gcagcggttg cgtatatctg
    49021 gatcgaccac tggctgcggt aacaaccccg gtgcatgaac gctgatggca ttcatcactg
    49081 cgtggcgtcc ggcaaaatgc tcttccggca gatagcgaca atcggaacgc ggataagtga
    49141 ttagcttgtg cgtttcgtac aacttctggc agatatcaag cacgttctgc gcgctcagac
    49201 caaagcgttt tgccgcttca atctgcaacg ccgaaagcga aaacggcagc ggtgcggatt
    49261 ctgattcccg tttatcgtta tagctggtga caatagccgg ttggccgctg atgcggttga
    49321 ccacatgctc cgccagcgga cgatgtaaca agcgcccttc ttcatcctga taaggttcac
    49381 acgcttcgct cggttgccag atagcggtaa accgctcatc ggcaggtgtc acgatatgcg
    49441 ctttgacttc aaagaaatct ttcgccacga agttttcaat ctcttcgtcg cggcgcacca
    49501 ccagtccgag tacgggcgtc tgcacgcgtc ccacggaaag tacgccctga taacccgcat
    49561 tgcgaccgag aatggtgtaa gcgcgggtca tattgatgcc gtacagccag tcggctcggg
    49621 ctcgcgccag tgcagaaacg cacaacggca caaactcact gttggaacga agacggtcga
    49681 tcgcccgctc aaccgcctgc gggttcaggt cgtttatcaa gcaacgctgc acctgctggc
    49741 gcttttccgg tgccagttgc agatagtcca gcacttcatc caccagtaat tgcccttcgc
    49801 gatccgggtc accggcgtga acgatttcgc tggcttcatg caggaagcgt ttgatgacat
    49861 taagttgttt ggtcacggag ggtcggggct gtaattgcca cttttccggg acaatcggca
    49921 aatcagcaag attccagcgc gcatagcggc tgtcgtaggc gtctggctgc gcctgctcaa
    49981 gcaggtgacc gatacaccag gtcaccacct gaccattacc gcactcgata aagccatcgc
    50041 ctttccggtg cggtttgggc aggacatcag caatggcgcg cgccagactc ggtttttcgg
    50101 caataaacaa ccgcattgaa ttaacggatc tcaaccatgg cacgaccgcc gcgcgctggc
    50161 accagttcgc caattgccgt cagttcaatg ccaaactcgg cggctgcagc tttaacctca
    50221 ttttctgctt ccggcatgac cgcaagcagc aaaccgccag aagtttgcgg atcgcacagc
    50281 agatcgcgca cttcacgcgg catttcaccc atcagatgac cgtagctggc aaagttacgt
    50341 tcggtgccgc caggcactgc gcccaactta atgtactctt caacccccgg cagtttcggg
    50401 atcgcttcat agtcgacgcg cgcctgcaca ccagcaccct gacacatttc gcttaaatgc
    50461 cccagcagac caaagcccgt aacgtcggtc atcgctttta cgccttcgat gttggcaaag
    50521 gacgcgcctg cgatgttcat ccggcacatc acttccgtcg ccagtccctg atgttctggt
    50581 ttcagcagtg attttttctc agccgtggta agaacgccga tccccagcgg tttcgtcagg
    50641 aacagtttgc atccggcttg tgcggtactg tttttcttca cccgctcggt cggtacgatc
    50701 cccgttaccg ccagaccaaa aatcggctcc ggcgcatcga tggagtgacc gccagccagc
    50761 gcaatacccg cctgacggca tgcatagcgt ccaccttcgg ttacttcgcg ggcaatttca
    50821 ggggaaagtt tgttaatagg ccagccgagg atcgcaatcg ccataatcgg tttgccaccc
    50881 atcgcgaaga tatcactgat ggcgttagtc gccgcaatgc ggccaaaatc gaaaggatta
    50941 tcaacgatcg gcataaagaa gtcggtggta ctgataacgc tggtgccatt gcccagatcg
    51001 tacaccgccg catcgtcgcg ggtttcatta cccacaagca aattcggatc aacaaacttc
    51061 gcctgctcac tgtgcaggat agtttccaac acttttgggg aaattttaca gccgcaacca
    51121 gctccgtggc tgtattgggt caaacgaatc gagttctcgc tcatggacat ctcctgtcaa
    51181 tgcaatccgg gtatggtaac cctcattccg tgaagtgata agtgagagtg tctgaattcc
    51241 tgcgcctttg ctcacaatcc agacagtttc gcgacaatta tcagaaataa gtgacaaacg
    51301 gcgtcgggtc cgggacgtta atcgacgtag atgctttcag ctgcggcgta ccaaggtaga
    51361 gaaaaccgac aattttatcc tgctcacggc aaccgaatgc ttcacgcact accggacttt
    51421 cagttaatgc gccactgcgc cagatgccgc caaacccctg ggcaactgct gccatttgca
    51481 tcgccatgac cgcgcatccc gcagacattt cctgttccca gcgcgggact ttatgatttt
    51541 cttcgcattt tgccaccacc gttatgatga gcggtgcgcg gaacggcgca ttacgcgctt
    51601 tgtcgatagc tttttcatca ctaccggcag caatcgcccc ctgttccaat aatgcgctga
    51661 aacgctcgcg tccttcccct tcaatcacaa aaaaatgcca cggttgcatg gacttatggt
    51721 ccggcgcacg catacccgct cgcagtatgt tttgcagttg ttcacccgtt ggcgcaggtt
    51781 cagccaggcg ggaggcgcta cggcgattga tcaatagttc gagtgcatcc atttgattaa
    51841 ctcctgtcat gatatttatt cacaaaatta acacgagagt ggattttgtt acagcacagt
    51901 ccgcaattcc tgctgacaag tgccggttgg gtcattacga taaccacatc tattgcgcct
    51961 gtgacaggtg tgaccttaag ttgggagaat acatgcgaac cctttggcga tttattgccg
    52021 gattttttaa atggacgtgg cgtctgctga atttcgtccg tgaaatggta cttaacctgt
    52081 tctttatttt tctcgtgctg gttggtgtgg ggatctggat gcaggtcagt ggtggtgatt
    52141 cgaaagaaac ggccagtcgt ggcgcgctgc tgctggacat atctggtgtg atcgtcgata
    52201 aacccgacag ttctcagcgg tttagtaaat taagccgcca actacttggt gccagttccg
    52261 atcgtctgca ggaaaactca ctgtttgata tcgtcaacac cattcgccag gcgaaggacg
    52321 accgcaatat caccggtatc gtgatggatc tgaaaaactt cgcaggcggc gaccagccgt
    52381 ctatgcagta catcggcaaa gccctgaaag agtttcgtga cagcgggaaa ccagtttatg
    52441 ccataggcga gaactacagc caggggcaat attatctcgc cagtttcgcc aataaaattt
    52501 ggctgtctcc gcaaggcgtg gttgatctgc acggctttgc caccaacggt ctgtactaca
    52561 aatcgttact ggataagctg aaagtttcca cccatgtgtt ccgcgtgggt acgtataaat
    52621 ctgccgttga accgtttatt cgtgatgata tgtcaccggc agcccgcgaa gctgacagcc
    52681 gctggattgg tgagctgtgg caaaactatc tgaatactgt tgccgctaac cggcagatcc
    52741 ctgctcagca ggtattccct ggcgcgcaag ggttgcttga gggtttaacc aaaaccggtg
    52801 gcgataccgc gaaatatgcg ctggaaaaca agctggtcga tgcactggca tcgagtgcgg
    52861 aaatcgaaaa aactctgacc aaagagttcg gctggagcaa gactgataaa aattatcgcg
    52921 ccatcagtta ttacgattac gcattgaaaa cgccggcaga taccggtgac agcatcggcg
    52981 tcgtcttcgc taatggcgca attatggatg gcgaggaaac tcaggggaat gttggcggtg
    53041 ataccactgc ggcacaaatc cgcgacgctc gccttgaccc gaaagtgaaa gcgattgtcc
    53101 tgcgtgttaa tagcccaggc ggcagcgtta ccgcgtctga agtgattcgc gctgaactgg
    53161 cagcagcccg ggcagcgggt aagcctgtgg ttgtatcgat gggcggcatg gcggcatctg
    53221 gtggttactg gatttccaca ccagctaatt acattgtggc taaccccagc accctgaccg
    53281 gttctatcgg tatcttcggc gtgatcacca ccgtagaaaa tagtctggat tcgattggtg
    53341 ttcatactga tggtgtatca acttcaccgt tggcggatgt ttctatcacc agggcactgc
    53401 cgccggaagc gcagcagatg atgcagttaa gcattgagaa tggctataaa cgctttatca
    53461 cgctggttgc tgatgcgcgt cattcgacgc cggagcagat tgataaaatc gcccagggcc
    53521 acgtctggac cggtcaggat gcaaaagcta acgggctggt cgatagtctc ggggatttcg
    53581 atgatgcggt cgccaaagca gcagagctgg caaaagtgaa acagtggcat ctggaatact
    53641 acgttgatga accgaccttc ttcgacaaag tgatggacaa catgtctggt tctgtccggg
    53701 caatgttgcc agatgcgttc caggccatgt tacctgcacc gctggcctcg gtagcctcta
    53761 ccgttaaaag tgaaagcgac aagctggcag cgtttaacga cccacaaaac cgttatgcgt
    53821 tttgcctgac ctgcgccaac gtgcgttaag tcttgtacta agtggccgac agatcgtcgg
    53881 ccacattttt tttacctcga cgaatcctct tcccgctgtc tcgccccata tttccttatc
    53941 cgcatagtat caggtgcgct ccccctgcct cacgtatata cttttgctct ttcgatatca
    54001 ttcatatcaa tatcatgcaa aagaaatcaa tttacgtcgc ctacacgggc gggaccatcg
    54061 ggatgcagcg ttccgagcag ggttatattc cggtgtcagg tcatctacag cgccaactgg
    54121 cgctgatgcc ggaattccat cgcccggaga tgccagattt caccattcat gaatatacgc
    54181 cgctgatgga ctcttcagat atgacgccag aagactggca gcatattgct gaagatatta
    54241 aagcgcatta tgacgactat gatggttttg tcattctgca cggcaccgac acgatggcgt
    54301 ataccgcctc tgcgctgtcg ttcatgctcg agaatctcgg taaaccggtt attgtgacag
    54361 ggtcacaaat cccgctagct gagttacgct ctgacggaca aattaatctg ctgaatgcgt
    54421 tgtacgttgc ggcgaattat cctatcaacg aagtaactct cttttttaat aatcgacttt
    54481 atcgtggcaa ccgtaccacc aaagcccatg ccgatggttt tgatgcgttt gcctctccaa
    54541 accttcctcc gttactggaa gcaggtatcc atattcgtcg tttgaatacg ccacccgccc
    54601 cgcacggtga aggggaattg atcgttcatc caatcacccc acaaccgatt ggcgtagtga
    54661 cgatttatcc agggatttct gctgacgtcg tgcgcaattt tctgcgccaa ccggtgaaag
    54721 cattgattct gcgctcatat ggcgtgggta atgcgccaca aaacaaagcc ttcctgcagg
    54781 aattacaaga agccagcgat cgcggtattg tggtggtcaa cctgacacaa tgtatgtccg
    54841 gtaaagtgaa catgggtggt tatgccaccg gtaacgccct cgcccatgcc ggcgttattg
    54901 gcggtgcaga tatgactgta gaagccacac taaccaaact gcattacctg cttagccagg
    54961 aactggatac tgaaaccatt cgcaaggcca tgagccaaaa cctgcgcggc gaactgacgc
    55021 cggatgatta aggagactgc aatgccccct cgcgccctgt tactggtcga tttacaaaat
    55081 gatttctgtg ctggtggcgc gctcgccgtg ccggaaggtg acagtacggt ggatgtcgct
    55141 aaccgcctga ttgactggtg ccagtcgcgc ggtgaagcgg ttatcgccag tcaggactgg
    55201 cacccggcga atcacggcag ttttgccagt cagcacggtg tagagcctta tacgccaggc
    55261 caactcgacg gtttgccaca aaccttctgg ccagatcact gtgtgcagaa cagtgaaggc
    55321 gcacaattac atccgttact gaaccaaaaa gcgatcgcag cggtgttcca taaaggcgaa
    55381 aatcctttag ttgacagtta cagtgccttt tttgataacg gccgtcggca gaaaaccacg
    55441 ctcgatgact ggttacgcga tcatgaaatc gatgaattga tcattatggg cctggctact
    55501 gactattgcg tgaagtttac cgtactggac gcgttacagt taggttataa ggttaatgtg
    55561 attaccgatg gttgtcgtgg tgtgaatatc cagccacagg acagtgcgca cgcgtttatg
    55621 gagatgtcag cagctggggc aacgctatat acgctggcag actgggaaga gacgcaggag
    55681 taattttatg ctggcctaca attctgtact gccattgtag gccagataaa acacgtcagt
    55741 ggtacatctg gcaattgacg ccatcaacga aagatttaat tcacctcaga aatctcttcc
    55801 aatgacacct tccgcgtttc gatgccaaaa atggagagaa ccagcgcaca gagtactaac
    55861 atcacgccaa gcactataaa caccgtgatt gacccataat gcgttaatag tgccgcaacg
    55921 ccataaggcg tgaagaccgc gacgatccgt ccgacggcat taacgaaacc tgaaccacgc
    55981 aggcgtaaat gcgttggcca aagctccggg atataaaccg ccgacgcgaa gcaaacatac
    56041 atgtataaaa agaagatcat caccaggcca tagattaaaa tcgcccactc tgtagtctga
    56101 attgaataga tatagcctaa cacggcaata ataataagta aggcggagcc aaataatcga
    56161 cgaggaaaat gatcaataat taatgccgca ataaatattc ctaccggagc gccaatcata
    56221 ataacagcag tcattaatat tgatttatca acatcaatgc cggagttaac aaatatggtc
    56281 ggtatccata cggtgatggt ataaagtgaa atgttcattg caattaaaac agtaatcgcg
    56341 actaatgtac gtcgtaacat ttccccttta aacagcagcc agaaagtacc attgattacg
    56401 ttagccttgc tgttgctctg attcaaagta agcgggggta aacgaacact cttctctctt
    56461 tcaatttgct gctctacttc acgaagttgg ctttctgcac ctgcgatttg tcctttcccc
    56521 gccaaccatc gaggcgactc aataaagtat ttacctgaga gaaaccaggc taacagtatg
    56581 ccaataccac ccagcagaaa cattatccgc caactaaaaa aagcgataac caccacgcct
    56641 atcgccgcag acaacatggg cgaccagtta ccgacaaatg agagccgcgc ggaccatttt
    56701 ccacgcaccg tcgcggggat aaactccgta aacgaagcat agccaaccat aatcagcgcc
    56761 cccattcctg tgcccatcag gaagcgaaag aagatgagcc agtacatatc agggacaaaa
    56821 gcggcccctg ttgccgcaat accgacgatg agaagattta tgcgaaaagc cctgcgccgc
    56881 ccaaaataat caccgatgaa cccaccggta agagagccga tgaaataacc aaacattaat
    56941 gccgaggtaa aggcggcatt gaggaaatta tttgaccatc cattgcttac cagctttgct
    57001 aaaacaacat tgcctgaata actcaaaaaa cccgttaata acagactaaa gcttataata
    57061 ccaaatatac gataatgaaa ccgggccaaa ggcaagcggt ccagtcttgc gccaatttga
    57121 tcatattgtt ccattaggtg cctcggattt gctaaaaagg tagcaattgc ggcaaattca
    57181 cttgccgcat ctttatcatc attttatttt ccaggcgcag gaacaaataa tattgacaac
    57241 gtgtgcttaa attaccacac cagctggata ttattgtctt tgcaaaatgc tatccactct
    57301 gcacccggtg atttatcagt tattatgtaa ttaatatgac taaagtcagc taactggaca
    57361 aaagctttgc gatcaaactt agagtgatca accaataacg caacctctgt cgcctgacgg
    57421 atcattgtct ttttgatttc agcttccgct tcgttagagt ccagcgcgcc gctgttaata
    57481 tctagacctt tacaactcat taccatgata tcgacatggt agcggctgat gatctcttta
    57541 gtaattcttc cttgcaggga aagtgtattt ttgtttagtt ccccacccgt tgaaacgact
    57601 ttaatttctg actgagccag aacatgaatc gcttccgccg agttggttag caacgtcagg
    57661 tcactacggt cctgtaacaa tttgagcaat tccattacgg tactactgga atcggctgcc
    57721 atggtggttt tattgtcgat aaagggtagt gccttgcgtg caataagctg cttctcttca
    57781 taaaacgatg aagcgcgctt ataaaaatgg atattctccg tcaacatcgc tgtatttaaa
    57841 acagcaccac cataggttct ggtcaaaaag ccttcatctt ccagcttctc aagatcgcgg
    57901 cgaatggttt cttcggttac ctgaaaaatc ccactcaaat ttgagactgt caccttttta
    57961 tcgttggcaa ccatttgctt aattgcctga atcctgtctt ttgccgccac gattacaccc
    58021 ctgtatcttt ttacatcaca ttagcacgat tatcgcataa ttgatgttta ctttcaaaat
    58081 aacctgtttg aatcacagat tttcatcaca gttttcacag aaacagaggt gaatcgtgtt
    58141 gagtatttaa cgctccaggg cctctgccat ttccctcatc aatgttgcgt ctgcatccga
    58201 taagttgata ttcaatgccg cgacattttc gcgtacctgt tccggtgcag tagccccact
    58261 aagaatggaa attaaatcac tctgttttaa tatccacgcc agtgccagag tgggaattgt
    58321 gcactgataa cgagcacaga gtggctgcca ctgttcaagc atatcaatca ctttcagcat
    58381 gttttcacgc tggaaccaga ctttatttgc ccgagcgccg cccgggacgt aatcacgagt
    58441 gatggtaccg gtcaacaatc cctgctctaa cggggaataa acctgtacca caatgccatt
    58501 atcacgacat agcggcagca gttcgctttc cattgcccgg tcgaggatac tgtatttcgc
    58561 ctgaataata tccagttcac catattgcag atactcgcgg atatggtcag catcgacgtt
    58621 agcagcgcct atagcgcgaa ttttcccttc ggcctttaac tcattgagca ctgcgacagt
    58681 ttcagcgatc ggcgtaaaaa atggcggcac cgactgccag tgcgtcatgt agatatcgat
    58741 gtaatcaata cccagacgtt gcaagctggc ttctacctct tcgcggatag attccgggga
    58801 aaggttttta tacaactgcc gatcgccaac tttgttgaat aaacttcctt ttcgttccca
    58861 gacaatgccg catttggttt ctactacaac ctgttcacgg ggcagttttt ttaacgcctg
    58921 accgacgata acttcactat tgccaaagtt atatcctggc gcagtatcaa tcagattaat
    58981 gccacaacga tgggcttcaa gaatcgtatc aatacatatt tgctgatcga gatcgccatt
    59041 ccatgcagga ccgccgccaa tggcccatgt ccccaacccc attcgcgaaa gcgtaatatc
    59101 cgttgtgcct aaaggtatct ttttcatttg ccttcattat ccttcgtatt cttccagcaa
    59161 ttgttcgacc agctttctgt tttttacgcc ggtggtggca ccgacgctta gaaccgagat
    59221 agccgccgtt gcattggcaa agcgtgcgca ttcacgcaga tttttgcctt ctaacagagc
    59281 cgctataaag ccagaagcaa agttatcgcc cgcgccaata gtgtcgatgg cggttattcc
    59341 tgcgaccgcc ggcaccttca tcgtcatgtc accacgcttg ataaagcagc cgtctttacc
    59401 cgttttaatc accaccgttt ttacgccgca cgcaagaaag cagtcagcaa tttcatccag
    59461 tgtctctttc ccggtgagta attttgcctc ggcaaaatta ggaaacagat aatcgacaaa
    59521 actcaatgct tcgcaaatat catccagcgt ttcattcaac cgtggtttga tcatatcggc
    59581 acagataatc atctgccggg ctttagcttg cgtaaaaatt tctgttagcg ctttaccatc
    59641 caatagagga ctgttgaaaa tactggccag tgataataat ttcgcctgag aaaaccgcgc
    59701 aaaatcaaca tcgtcaatat tcaatttcca cagactaccg ttacggtttg tgacaaacgt
    59761 ccgctcgcca tcctccgtca ccagtccaac gttaatagag gtatctatgc tgacgtcctg
    59821 cttcaggctt tgaatatcaa tattctcttt gcggcaatgg tcgagaataa attgtcctgc
    59881 ggcatcttta ccaacacgac tcattaatgc tgtgcgatgg cccagacgag aaataattgt
    59941 tgcttcattt attgcatcac ccccggtggt cattgcgatt ctttcaagag gataagaatc
    60001 cacatcaaag atatttttac tgaccggttg caatggaata tcaacaatag cggcacctat
    60061 acaaataatg tcgagattat ccatatcatt attccgcttt gccatcagaa ccgaacagtt
    60121 taattttttc caatgcccgt tcttttacag ctttacgtac ttcacgttct aaatgcagga
    60181 aaggctgatc ctggttttct ttcactgcta ccattgcggc ctgacagagc tccgtatgaa
    60241 tgttgatttt cgcgataccc aacgaaattg cagttttaat gtcggtatca ctgatccccg
    60301 atgcgccgtg caaaaccagc ggaacagaaa cggcatcgcg tacgcgtttg acgacctcaa
    60361 agttcaattg tggctctgac gtataaaccc catgctggtt gccgatggcg acagccaatg
    60421 agtcacagcc cgtacgttca acaaattcag ccgcctggtc aggatcggta tagtgatagc
    60481 ccgccagcgc ttcttcataa acggtttcat taccgacatg ccctaattct gcctctaccg
    60541 gaatacccag cggatggaag aaatcaacag cctctttggt taaacgaata ttttcttcga
    60601 aatcaaatgc ggaagcatca cgcattaatg aattcatacc atgagtccag gcgttatgaa
    60661 taatttccat actccgacca tgatcccaat gagttattac cggcaccgtt gctttttttg
    60721 ccattgatac catcatgtga gagaaatctt caaatgaggt gttcccgaca aaacctgtac
    60781 caaaagaaat aataaccggt gatttcgctt cttcggctgc gtcgataacg cccatcaaca
    60841 tttctgcatt ccatacgtta aaatgggcaa ttgcataatg tttatttgta gcatcgtttt
    60901 cccaatatct gatatctgcg agcatattct gttctcccgt attaattaat cgctaatttt
    60961 aataacgcct ttaataatgt cgcgtttgtt gttaaccgac tcttcaaatg cctgttgtac
    61021 atcctgataa tcgtaaatat gtgtcaccat cgatttcaca tcgaatcgcc ctgaggaaat
    61081 agcttcaatc gtgaccggat aacgattggc atagcggaat actgtctgaa tagtgacttc
    61141 gcgattgatt ttgaggaaat tgattgccga atcgcccggt acagtaccaa caatcataat
    61201 tttaccgccg cgcattacca gataaggtgc ctgtttaacg gtgaccgcag aacccgctgt
    61261 ttcgaaaaca atatctgcgc ccatttcttc ggtaaattgc tgacagcgtg caatagtgtc
    61321 ttcttttgcg ccgttaataa ccactgtcgc gccgagctgt tccgccattg tcagacgttt
    61381 ttccagtaca tcaacgacgg caatatccgt agctcccagg catttgcacg cttgcaacgt
    61441 catcaaacca atacaacccg ctcccagaat aattatcttc ttacccggtt taacatctgc
    61501 cagcatcgcg gcatgcatcc cgactgcggc aggctccacc agcgcccctt ccatcgtgtc
    61561 catattgtcg ggcagtttgt aagtaaagct ctccggatga cacagatagt gcgttaatgc
    61621 gccgcggtag ttaggttgtg tcgccataaa atcaacgtcc gggcagatgt tatatttgcc
    61681 ttccagacag taacgacagt gaccgcaagg aacgccaggt tcgatattta ctcgatcccc
    61741 cgctttaaat ttgcgcactc ggcttcccac agccacaacc gtcccggcgc attcatgacc
    61801 caggccaatt tcttgatttg ggtctttagg cggaataaac gggcctgatt caaaaccatg
    61861 tacatctgaa ccacaaatac cgacatattc cactttaatc aaaacttcat cttctttagg
    61921 cacaggtatt tctgctgaaa taattttcat tgtgcccggc acctgcaata ttgcttttga
    61981 atttttcatt ttgaatccac cagtattatt tattgactac tgcatcaata cttaaacttt
    62041 caacggagac acctttggtt tcaattccaa tggtggcgat agcaattgcg acaataattg
    62101 aaaccgcccc cagaagaata aagactcccg ttacgccata actactgagc agcactgcaa
    62161 cggcataagg tgcggcaata ccactgattc gccctactgc attcgccaga ccggagccac
    62221 gtaatttggc ctctgtcggc cagatctcag ggacatacac tgccgaggca tagcaaacgt
    62281 acatatagac gaaagtaatc aggaagaaac caatcagcgt tatgagcaac atactggttt
    62341 gcagtgaata gatatatccg agcaccgcaa tcaggattaa tagccccaca cccattgttt
    62401 tacgcggaat tttatccatc accagcatgg caataaaaat accaaatggc gcaccaaaca
    62461 tactcatggt atttaaaaca atcgagtctt ttaaattaat cccctgggtc atgaatattg
    62521 ttggcaacca gttaattagt gtgtactgca caacgttcat ggcaatcagc acacaagaac
    62581 ctaatatcac gcgtttcagt aatactcctg tcagtaaggc tgaatacggc accgcttgtg
    62641 gcgctttacc gtcatcagca ataaccacgg gcggcaaagg ttttcccgtc tggcgtatga
    62701 cgccttcttc tatactccgc atgactttct cggcttcctg ataccgtccg cgcgattcca
    62761 gccagcgcgg ggattcagga aagtagcgcc aggccagcgc cgtagcaata agcgacaata
    62821 ttgcagggat aagcagttgt actcgccagt tccactctgc actaatcaga ggcgtgagtc
    62881 ccatcgcaat caatgaacag agcggatatg accagttgcc aataaaagaa acccgactgg
    62941 accacgttcc gcgatttcta ccgggcatat attcggtgaa accagcaaac agcgtcacca
    63001 gtaaagctcc cagcccaacg cccatcacaa aacggcaggc aataaggaaa tccatattcg
    63061 gtgaaaaagc accgaccacc atcgaggcaa tatgaatggc ctcatagagg atgaacgcat
    63121 ttctgcgacc ggttttgtca ccgatgatgc cgccaaccag cgcaccgaga aacattccgg
    63181 cggtcgtgat tgctgagaat gtggcagtgg tggaattatc tgtccagccc aactctttca
    63241 gctgcgcgag gatcaagcca ccaacggcat tactccagca gacaagcaag ccaaacgaga
    63301 cgatggcaaa tattgacgaa tgccagcggc aatccggtaa gcgatccagc cgtgcaccac
    63361 aatgcggttt tgttatctgt tccatgcgat aacgttcctt caggagagta gttattcatc
    63421 aaagtcgtaa gtcatgatca ctttgattgc ggttttatcg accatcgcat caaacccttc
    63481 gcgccattgc gacaggccga tacgatgcgt gatcatcggt ttgactttga tagcgccgct
    63541 ggctaatagc cggagagcgt tacgccatga ggtggagtca taggccatat gcccaatgat
    63601 gcttttgttc caggcggtaa tgtcattaat cgagaaatca agaggtttga agcccattcc
    63661 aacgcgtacc acttccccgt tcgggcggag catttcgatg gcttgtttca atgcgatatt
    63721 ggcaccggag cattcaatca ccagtcccag attgtctttg ccacaaattt gctggcagcg
    63781 cgccaccaca tcttcggtag aaccatttac tacagccgtc gcacccagtt cttttgcaac
    63841 cggaaagcgg accgccacat cttcttgcag accaacgacg acgatattta ccgcccccat
    63901 aatccgcgcc atttgtacgg agaacagccc gagtgggcca gtgccgatga cgaccacatc
    63961 ctgaccagga aggaatttcg attgctgcgc gatggatttg taggcattac agatagggtc
    64021 aagtacggct gcgtcctcat aatcaacacc atcagggatt tcccacaacg catgacgatg
    64081 aattttgaga atttcaccag gaaccagaca atatttggaa aaaccaccgc cccaggtatt
    64141 gttatccaga ccaaggttta ctttttctgt acaacacaga aaatcacctt gttcacaggc
    64201 cggacaaacg ccgcagacgt gaccgctgtt atctgacacg acgcgttgcc ccactttcca
    64261 gtctttgact ttttcaccaa cctgcgcaat acaacctgcg aactcatggc cgcggataga
    64321 gttaaactca tcagaaccgc tatcgacatt gtagtgcttc atgtctgcgc cgcagattgc
    64381 cgcggcttta atttcaatca ctacatcttc cgggccacaa atgggttgtg gcacatcgat
    64441 catcttgtag ccgccaaagg ctttgccaaa ccgagccagt gctttcattg cgtcatcctc
    64501 tgtgttttgt tttgcttaga tggtcatcat acttacccac atgatttttg aggtcaacat
    64561 taaatctgta aaacacagat aatgatctgc gttttacaac tcagatcaca attacgcaga
    64621 aaatggctgg atctgttaca ggttgatgga aggcggaacg caaaatgagc aaatttgaga
    64681 gggattaccg gccaaatagg tctgagactt aagttcagga gaagtcattc aggcgtgaac
    64741 gcgactgcaa gagaccgggc tggggctttg cctggtcgtg acgagataat tgagatgagt
    64801 cgtggggtta ttgttggcta tcccctttat aaccgcggca aaaaggggac aaaccactta
    64861 cttaaacatg gcaataggct ttgctgagat accaaagtct tcttttaact gttgtttgct
    64921 cttcatgacc atctgaccat ttgtgtcaat tgtcatatgc tgtgcttcgg tattattgcg
    64981 ggcttgccac agcatcacca gttgcaggca gttctctttt tgttcctcgg tcaacgcaac
    65041 gccatcaggc catttcccca attcaacggc ggtcgacaaa cgctggtata cctcaggcgt
    65101 catgctgttg ataatgtcat caagattcat aatttttccg ctcctgtgga ataagttgct
    65161 gaatcgtttt ttcaaccgtt gatttcttcg ccgttttcgc catcggtaaa gcgtaaagag
    65221 gcagagttaa cacaataacg ttcgcccgtt ggctgcggcc cgtcggggaa gacatgcccc
    65281 agatgggcat cacagttacc gcaacgaatt tctatgcgct gcattccatg tgacaagtct
    65341 ttgatataac gaatggattc ttcactcacc ggttcgtaga aactgggcca gccacagccg
    65401 gaatcatact tggtttggga atgaaacagc ggggcatcgc agatcaaaca gtgatatacg
    65461 ccgtcacgct tgttatgcag taaacgaccc gtaaatggcg gctcggtccc atgattctgc
    65521 gtcacgtaga actgcatctc ggacaaattt tttttcagtt cttctgccga aggtttatta
    65581 gccatttgct cacatctcac tttaatcgtg ctcacattac gtgactgatt ctaacaaaac
    65641 attaacacca actggcaaaa atttgtccta aacttgatct cgacgaaatg gctgcaccta
    65701 aatcgtgatg aaaatcacat ttttatcgta attgcccttt aaaattcggg acgccgaccc
    65761 catgtggtct caagcccaaa ggaagagtga ggcgagtcag tcgcgtaatg cttaggcaca
    65821 ggattgattt gtcgcaatga ttgacacgat tccgcttgac gctgcgtaag gtttttgtaa
    65881 ttttacaggc aaccttttat tcactaacaa atagctggtg gaatatatga ctatcaaagt
    65941 aggtatcaac ggttttggcc gtatcggtcg cattgttttc cgtgctgctc agaaacgttc
    66001 tgacatcgag atcgttgcaa tcaacgacct gttagacgct gattacatgg catacatgct
    66061 gaaatatgac tccactcacg gccgtttcga cggtaccgtt gaagtgaaag acggtcatct
    66121 gatcgttaac ggtaaaaaaa tccgtgttac cgctgaacgt gatccggcta acctgaaatg
    66181 ggacgaagtt ggtgttgacg ttgtcgctga agcaactggt ctgttcctga ctgacgaaac
    66241 tgctcgtaaa cacatcaccg ctggcgcgaa aaaagtggtt atgactggtc cgtctaaaga
    66301 caacactccg atgttcgtta aaggcgctaa cttcgacaaa tatgctggcc aggacatcgt
    66361 ttccaacgct tcctgcacca ccaactgcct ggctccgctg gctaaagtta tcaacgataa
    66421 cttcggcatc atcgaaggtc tgatgaccac cgttcacgct actaccgcta ctcagaaaac
    66481 cgttgatggc ccgtctcaca aagactggcg cggcggccgc ggtgcttccc agaacatcat
    66541 cccgtcctct accggtgctg ctaaagctgt aggtaaagta ctgccagaac tgaatggcaa
    66601 actgactggt atggcgttcc gcgttccgac cccgaacgta tccgtagttg acctgaccgt
    66661 tcgtctggaa aaagctgcaa cttacgagca gatcaaagct gccgttaaag ctgctgctga
    66721 aggcgaaatg aaaggcgttc tgggctacac cgaagatgac gtagtatcta ccgatttcaa
    66781 cggcgaagtt tgcacttccg tgttcgatgc taaagctggt atcgctctga acgacaactt
    66841 cgtgaaactg gtatcctggt acgacaacga aaccggttac tccaacaaag ttctggacct
    66901 gatcgctcac atctccaaat aagt
//
LOCUS       DAPOZO010000013        66166 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPOZO010000013 DAPOZO010000000
VERSION     DAPOZO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.013"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(445..3228)
                     /locus_tag="ROI21_000891"
     CDS             complement(445..3228)
                     /locus_tag="ROI21_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDX5109850.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWGNAATFDKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLEREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVSDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSTRTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRKLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRINIREQASG
                     AYSVSSRLSVDPQAKDISHSLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTTVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3285..5657)
                     /locus_tag="ROI21_000892"
     CDS             complement(3285..5657)
                     /locus_tag="ROI21_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDX5109851.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLAFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTAVS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPESDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KYAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNRVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5695..7380)
                     /locus_tag="ROI21_000893"
     CDS             complement(5695..7380)
                     /locus_tag="ROI21_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDX5109852.1"
                     /translation="MITIPITFRMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGKHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKDNWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLSVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            7583..7705
                     /locus_tag="ROI21_000894"
     CDS             7583..7705
                     /locus_tag="ROI21_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5109853.1"
                     /translation="MTKHPTVIYVGCLVKAIRTKLRLEQRGSVINYSPFVLQHP"
     gene            complement(7671..8828)
                     /locus_tag="ROI21_000895"
     CDS             complement(7671..8828)
                     /locus_tag="ROI21_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDX5109854.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLSNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCESYKVLFSTMVPYMNAMVELAKNRVPLYHIMDVARQMENN"
     gene            complement(8880..10562)
                     /locus_tag="ROI21_000896"
     CDS             complement(8880..10562)
                     /locus_tag="ROI21_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDX5109855.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENRDVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            complement(10964..11725)
                     /gene="ydeO"
                     /locus_tag="ROI21_000897"
     CDS             complement(10964..11725)
                     /gene="ydeO"
                     /locus_tag="ROI21_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDX5109856.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTDNTMSALAI"
     gene            complement(11800..11997)
                     /gene="safA"
                     /locus_tag="ROI21_000898"
     CDS             complement(11800..11997)
                     /gene="safA"
                     /locus_tag="ROI21_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDX5109857.1"
                     /translation="MHATIVKNKITQRDNYKRIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            complement(12245..14524)
                     /gene="ydeP"
                     /locus_tag="ROI21_000899"
     CDS             complement(12245..14524)
                     /gene="ydeP"
                     /locus_tag="ROI21_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDX5109858.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFIPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTHLDLTVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            complement(14863..15777)
                     /locus_tag="ROI21_000900"
     CDS             complement(14863..15777)
                     /locus_tag="ROI21_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5109859.1"
                     /translation="MGKTISMKLLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            complement(15837..16340)
                     /locus_tag="ROI21_000901"
     CDS             complement(15837..16340)
                     /locus_tag="ROI21_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX5109860.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(16353..16883)
                     /locus_tag="ROI21_000902"
     CDS             complement(16353..16883)
                     /locus_tag="ROI21_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX5109861.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            complement(16897..19548)
                     /locus_tag="ROI21_000903"
     CDS             complement(16897..19548)
                     /locus_tag="ROI21_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX5109862.1"
                     /translation="MTAFHAAFKAYRMHQVLILPRFARLTFALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHLAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSIKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMNGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            complement(19590..20300)
                     /locus_tag="ROI21_000904"
     CDS             complement(19590..20300)
                     /locus_tag="ROI21_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDX5109863.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            complement(20661..21224)
                     /gene="fimA"
                     /locus_tag="ROI21_000905"
     CDS             complement(20661..21224)
                     /gene="fimA"
                     /locus_tag="ROI21_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDX5109864.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(21658..21924)
                     /locus_tag="ROI21_000906"
                     /pseudo
     CDS             complement(21658..21924)
                     /locus_tag="ROI21_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(22327..23130)
                     /locus_tag="ROI21_000907"
     CDS             complement(22327..23130)
                     /locus_tag="ROI21_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDX5109865.1"
                     /translation="MKSVLAAAAMSLVISDFANAEETRGKAMMKIEPSAISEADIRSV
                     SPALARFGREAITEDLWTRDALSPRDRSVVTVAMLIARNQPAELKHYIDVALDSGVTP
                     AELSEIITHLAFYSGWPNAMSAVSVTKAVFETRGVTADALPEASPDLLPLNQEAEKQR
                     SETVEKNVGPISPGLVKFTADPLFLDLWQRPALKPRDRSLITVSALIASGQSAQIGYH
                     LNRAMDNGLSVEEAGEIVTQAAFYAGWPNAFTAAPVVGEVLNNRSSSKR"
     gene            complement(23146..23841)
                     /locus_tag="ROI21_000908"
     CDS             complement(23146..23841)
                     /locus_tag="ROI21_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDX5109866.1"
                     /translation="MLISSPVFATKPDAPNKPEMNIFELGIRPDRDNDFADVARQTIS
                     ASVDHEAGTLAMYALQRSDNSGQAFMFELYENERAYRKHLNAAPYKAFAARAPDIIDR
                     KKKITLEPQFLGDKHIIPNERTINNLVIVEVKPEFQSEFRNIVLPEMVESLKVEKGVL
                     AMYAGTDSETPKRWYFYEIYASEEDYQLHRQTLHFLDYLRQTAHMSARKDAIPVKPVF
                     LRNKGGIKQEPQR"
     gene            complement(23987..25006)
                     /locus_tag="ROI21_000909"
     CDS             complement(23987..25006)
                     /locus_tag="ROI21_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDX5109867.1"
                     /translation="MQYTRLGKSDLLVSRICMGCMGFGDPSTGQHSWTLDETASRDII
                     RYGLEKGINFYDTAIAYQNGSSERYVGRALREMAKRDDIVLATKFLPRTPAQIAGGIS
                     GKEAIAQSLDQSLKNLGMDYIDLYIYHIWDYNTPIIDVLEALHTAVTAGKVRSVGISN
                     CYAWQLAKANALAEREGLTPFVSVQSHYNLIMREDERELFGLCTEDNIAMTPYSALAS
                     GRLSRKEGYTRRATEDDYARGKYDSTAEQDRSIIERVAELAERHLVSMTEISLAWLLT
                     KVTSPVVGATKKAHVDGAVNAVNLQLSPEEIQFLEETYQPHVLTGGMAQNTPQTKDVK
                     QVWMR"
     gene            complement(25009..25581)
                     /locus_tag="ROI21_000910"
     CDS             complement(25009..25581)
                     /locus_tag="ROI21_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDX5109868.1"
                     /translation="MKNILIVSGHPELTHSVANATILDEVETALPDVEIRRLDRLYPD
                     GKFNIAAEQESLLRADVIVWQFPFSWYGLPGLMKKWLDEVFVHGFAHGSTAKLGGKKL
                     ILSFTTGAPEELYTVDSFFGHTIEEYLIPFETTARLCNLELLAPVYTCGISYADRDAD
                     KIAQQKTLAREHASRLVELLNSVVNNPEGE"
     gene            complement(25732..26410)
                     /locus_tag="ROI21_000911"
                     /pseudo
     CDS             complement(25732..26410)
                     /locus_tag="ROI21_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013814197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            complement(26513..27460)
                     /locus_tag="ROI21_000912"
     CDS             complement(26513..27460)
                     /locus_tag="ROI21_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDX5109869.1"
                     /translation="MKTLPPVALGTWSWGTGFAGGDTVFGNHLSDTQMAEVFTAAMSK
                     GLNLWDTAAVYGMGSSETALGALVRQFPRENIILSTKFTPQIADKQSAQPVSDMLEAS
                     LGRLGVDAIDIYWIHNPLDVEKWTPGLIPLLQSGKVKRVGVSNHNLAQIRRANEILNA
                     SGYSLSAVQNHYSLLYRASEEAGILDYCRQNNITFFAYMVLEQGALSGRYDSNHPMPA
                     GSGRAESYNAVLPQIERLTAAMKKMGAERNASVAQIAIAWAIAKGTLPLVGATKVHHV
                     LDAACASDIQLRDEEIILLEQLAAETRVDTRGAWEKPMV"
     gene            complement(27534..28223)
                     /locus_tag="ROI21_000913"
     CDS             complement(27534..28223)
                     /locus_tag="ROI21_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDX5109870.1"
                     /translation="MKPAYKPVLCVVSSHPIKGASGVPTGFFLAELTHPLKVLEDAGI
                     KTTIASIRGGQPPVDGFDLSDPVNAWYWNETDFQQRLATTPALSELKGADYSAVFFAG
                     GHGTMWDFRDNQDAQRIIREVYESDGIVSAVCHGPAALVDAKLSSGEYLIKGKNVAAF
                     TNKEEEEVHTTDVVPFLLETALREHGALYHEAPNWSENVVTDDRLITGQNPASAHGVG
                     VALLNALRRTA"
     gene            complement(28232..28540)
                     /locus_tag="ROI21_000914"
     CDS             complement(28232..28540)
                     /locus_tag="ROI21_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDX5109871.1"
                     /translation="MRILCLDIPAPGASLEKYAPHLNAEALHAWGLYKSGFIRDIYFR
                     QDRPGVAIFLECDSVDEAMNVMAEFPLAKAGLLSFECIPLGSFINWENLFADEFKNKE
                     "
     gene            28679..29578
                     /locus_tag="ROI21_000915"
     CDS             28679..29578
                     /locus_tag="ROI21_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5109872.1"
                     /translation="MNNALYNQIRIFQSIAREGNISAAARKLEITPPSVSNALKLLEE
                     YIGHPLFVRTTRRIELTETGQLLLEQTAAAVESLENSLESIRDQHQEPSGMVRITLSR
                     FAYLLILKPAMAEFCQQYPGIQLEISVYDGTVNIIKERFDLGIRFGDILEGGGVARPL
                     MKPFREGLYASSAYLSRHGTPEVPADLCHHQLIGYRFITNNRILPLLLNDRGEQLTVE
                     MPGQLISNDIDVMADGIRNGLGIGRLFEPVWQLQPDRERFIPVMESYWKTYPPVYLYY
                     PKNAGKTKRVKALIDFLISTTGR"
     gene            29945..30827
                     /locus_tag="ROI21_000916"
                     /pseudo
     CDS             29945..30827
                     /locus_tag="ROI21_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(30828..33468)
                     /locus_tag="ROI21_000917"
                     /pseudo
     CDS             complement(30828..33468)
                     /locus_tag="ROI21_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310144.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33992..35584)
                     /gene="lsrK"
                     /locus_tag="ROI21_000918"
     CDS             complement(33992..35584)
                     /gene="lsrK"
                     /locus_tag="ROI21_000918"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDX5109873.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFNLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSDIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(35663..36616)
                     /gene="lsrR"
                     /locus_tag="ROI21_000919"
     CDS             complement(35663..36616)
                     /gene="lsrR"
                     /locus_tag="ROI21_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDX5109874.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEHETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            36865..38400
                     /gene="lsrA"
                     /locus_tag="ROI21_000920"
     CDS             36865..38400
                     /gene="lsrA"
                     /locus_tag="ROI21_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDX5109875.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTIELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHTAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            38394..39422
                     /gene="lsrC"
                     /locus_tag="ROI21_000921"
     CDS             38394..39422
                     /gene="lsrC"
                     /locus_tag="ROI21_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDX5109876.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSMQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILMAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            39422..40414
                     /gene="lsrD"
                     /locus_tag="ROI21_000922"
     CDS             39422..40414
                     /gene="lsrD"
                     /locus_tag="ROI21_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDX5109877.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            40426..41448
                     /gene="lsrB"
                     /locus_tag="ROI21_000923"
     CDS             40426..41448
                     /gene="lsrB"
                     /locus_tag="ROI21_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDX5109878.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLMDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKNSV
                     YVADELLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            41475..42350
                     /gene="lsrF"
                     /locus_tag="ROI21_000924"
     CDS             41475..42350
                     /gene="lsrF"
                     /locus_tag="ROI21_000924"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDX5109879.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMLTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            42373..42663
                     /gene="lsrG"
                     /locus_tag="ROI21_000925"
     CDS             42373..42663
                     /gene="lsrG"
                     /locus_tag="ROI21_000925"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDX5109880.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLEALMTGPRKKRLFNGLMP"
     gene            42720..43478
                     /gene="tam"
                     /locus_tag="ROI21_000926"
     CDS             42720..43478
                     /gene="tam"
                     /locus_tag="ROI21_000926"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDX5109881.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(43482..44396)
                     /locus_tag="ROI21_000927"
     CDS             complement(43482..44396)
                     /locus_tag="ROI21_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family inner membrane protein"
                     /protein_id="HDX5109882.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(44593..46044)
                     /gene="uxaB"
                     /locus_tag="ROI21_000928"
     CDS             complement(44593..46044)
                     /gene="uxaB"
                     /locus_tag="ROI21_000928"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDX5109883.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     QDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIATG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELCLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDVILEKGMREAVRPLC"
     gene            complement(46272..47690)
                     /locus_tag="ROI21_000929"
     CDS             complement(46272..47690)
                     /locus_tag="ROI21_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDX5109884.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARIGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(47829..48188)
                     /locus_tag="ROI21_000930"
     CDS             complement(47829..48188)
                     /locus_tag="ROI21_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDX5109885.1"
                     /translation="MQSLDPLFARLSRSKFRSRFGLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVIQMNQP"
     gene            complement(48188..49114)
                     /gene="glsB"
                     /locus_tag="ROI21_000931"
     CDS             complement(48188..49114)
                     /gene="glsB"
                     /locus_tag="ROI21_000931"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDX5109886.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(49178..50566)
                     /gene="sad"
                     /locus_tag="ROI21_000932"
     CDS             complement(49178..50566)
                     /gene="sad"
                     /locus_tag="ROI21_000932"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDX5109887.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRVRSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQAGQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            50667..51542
                     /locus_tag="ROI21_000933"
     CDS             50667..51542
                     /locus_tag="ROI21_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5109888.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAQFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQS"
     gene            51626..52741
                     /gene="yneK"
                     /locus_tag="ROI21_000934"
     CDS             51626..52741
                     /gene="yneK"
                     /locus_tag="ROI21_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDX5109889.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNISSATKKIFNKLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPDRNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMINPGVPIKRAHTPHIEPLHPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFGNKEPNLLTAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQD
                     FPHFQDLFLFFANMLLHYHQFMNPNESDLIDVLMPASLSDDKIIKHMARKTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            52891..54081
                     /gene="ydeA"
                     /locus_tag="ROI21_000935"
     CDS             52891..54081
                     /gene="ydeA"
                     /locus_tag="ROI21_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDX5109890.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGAFITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(54106..54771)
                     /gene="marC"
                     /locus_tag="ROI21_000936"
     CDS             complement(54106..54771)
                     /gene="marC"
                     /locus_tag="ROI21_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDX5109891.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            54983..55417
                     /gene="marR"
                     /locus_tag="ROI21_000937"
     CDS             54983..55417
                     /gene="marR"
                     /locus_tag="ROI21_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDX5109892.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKRVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            55438..55821
                     /gene="marA"
                     /locus_tag="ROI21_000938"
     CDS             55438..55821
                     /gene="marA"
                     /locus_tag="ROI21_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDX5109893.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            55853..56071
                     /gene="marB"
                     /locus_tag="ROI21_000939"
     CDS             55853..56071
                     /gene="marB"
                     /locus_tag="ROI21_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDX5109894.1"
                     /translation="MKPLLSAIAAAFILFSTQGIAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(56128..57567)
                     /locus_tag="ROI21_000940"
     CDS             complement(56128..57567)
                     /locus_tag="ROI21_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDX5109895.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLKFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(57592..59265)
                     /locus_tag="ROI21_000941"
     CDS             complement(57592..59265)
                     /locus_tag="ROI21_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDX5109896.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNALATKLTIEQRLELLENELSQ
                     NKQELRATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFTRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDDSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVVRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(59321..59632)
                     /locus_tag="ROI21_000942"
     CDS             complement(59321..59632)
                     /locus_tag="ROI21_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDX5109897.1"
                     /translation="MFADEELVMELLINAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ELACLQAHKIQTALISQDEGCGKIVVNLILVHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(59660..60982)
                     /locus_tag="ROI21_000943"
     CDS             complement(59660..60982)
                     /locus_tag="ROI21_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDX5109898.1"
                     /translation="MGLMTSFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVSRSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYKLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFILVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(61097..61408)
                     /locus_tag="ROI21_000944"
     CDS             complement(61097..61408)
                     /locus_tag="ROI21_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDX5109899.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            61607..62305
                     /locus_tag="ROI21_000945"
     CDS             61607..62305
                     /locus_tag="ROI21_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDX5109900.1"
                     /translation="MIFQKIARLLKSEINGYSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFNEIAHKSGKEAGSQIMKFEVQD
                     ASPPIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVAHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(62350..63249)
                     /gene="eamA"
                     /locus_tag="ROI21_000946"
     CDS             complement(62350..63249)
                     /gene="eamA"
                     /locus_tag="ROI21_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDX5109901.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            63444..64631
                     /gene="ydeE"
                     /locus_tag="ROI21_000947"
     CDS             63444..64631
                     /gene="ydeE"
                     /locus_tag="ROI21_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDX5109902.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYNLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSAWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTNLPPSSLFIILTLVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            64758..64853
                     /gene="mgtS"
                     /locus_tag="ROI21_000948"
     CDS             64758..64853
                     /gene="mgtS"
                     /locus_tag="ROI21_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDX5109903.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(65072..65962)
                     /gene="dgcZ"
                     /locus_tag="ROI21_000949"
     CDS             complement(65072..65962)
                     /gene="dgcZ"
                     /locus_tag="ROI21_000949"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDX5109904.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLRPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGIRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT        17438 a        15768 c        15085 g        17875 t
ORIGIN      
        1 tctgcgatag tggaaataga cttcgcacca aaacgtgaat cgagtagttc cgaccttaaa
       61 tccgttactt gcttcttatc cattttaaac tccttaaaat gattggatcg cattaaaaaa
      121 gtaggattta tcgataaagt aagcaagtgc aaaggactcg ggtttaaata acaaaatcct
      181 aatgttattt atcgtgaaat attacgagaa taatgttttt tcattaaaaa acaatgcaat
      241 agagaatttt ttggtggtgg taaagtgttt tatgttgtta tttttatgcg ctttctacgc
      301 ctcctgaatt atcacgtaaa aatcagtcct taaaatatca ctattagtac ttggttatta
      361 ttttgaacgc atttataaaa ttattacata aaaatagcga atgttgataa aatccccgac
      421 aacgatgtgt tgacggggct gttattattt tggcaataat actccggtat aagtatttac
      481 cggatgagaa agatattgtt taacggtagt gttaacattc tctaccgtca tttgtttcaa
      541 caattgctcc tgctcagtcc atgctgcagg atcgtcatat tgaataagac tatttacaat
      601 ggtgttcgct aattgttgaa cgctacgctg ttggatatcg aggctgcgct gaacgttttg
      661 ctggtattcg ttcagttctt gctcactgat ccctttagcc agacgtttaa ccatcacttc
      721 attcgctaac gttaacagtt catcatgtcg ttccggttga caagtaaaag ccagcgaatg
      781 actgatatct ttggcctgag gatcaaccga gaggcgagaa gaaacgctgt atgccccaga
      841 tgcctgttca cgaatattaa tgcgtagatc ttttgccagt gcgacgttaa aagcatcgag
      901 cgccatacgc gtcgccagat taaccggagt ccgggaatca taacgcttcc actgtgaaac
      961 ctgtgccacg ggttcatttt gttcttttac cgtaaccgat gcattgtccg tcgcgcgagt
     1021 taatggttta cctgcggcta atggcgaatc agagtgtttg attgatccta agtaacgcgt
     1081 aattaacgcc acgagtttgt cttctgcgac attaccgaca atgacaaacg tgatatccgc
     1141 tggagatgaa aacaacttgc gatcggcagc cagcgcatct gcggcagtaa actgtgcaat
     1201 ctgattttct tgcagtaatt tcgtgcggtc atcagcatag cgcgtctcat acatctgctg
     1261 ggcgaatttc tccgctggac gctggtcgag tgttttcaac gcctgaattt gagcattttg
     1321 tagcgatgcc caaatattat cgttaatcgt gctgtgggtg attcgctggt taatcaactg
     1381 gaaaccaggt tcagggttat tagtccgcgt gctaacagag agcaatgtat tcatgccact
     1441 gactttactg ctcatggtta ccgagttttc cgcactccag cgtttcaggc tggaagagga
     1501 gagttcacca acgccgcttc cgcttactgc tttatttgcc agcgcgataa gtgatttttg
     1561 ctgcgcaggg aaacttaaat cgcctttatt agatacggca gtaatttgta gcttttgctc
     1621 ttcacccgcg gattttgcca gaataaccct ggcaccattg gaaagtgtta atgatgtcag
     1681 attctcagcc agcgtttctt tgctgctaat ttccgcctgt gggtcagcgt ctactgttaa
     1741 cgataaattt ctgcctggga agatgtaagc cgccagcttt ttgttggcgt actctttttc
     1801 cagcgccagg atagccgcag gagacaatgc ttttttggca gcaacctcat tgtttaccat
     1861 ttgctcccaa aatgcatcct ggttctttct taactgctgc catttttccg ccagactttg
     1921 cacggtaatt tgctgccaca gacgtttcga aagttgatat gtctcttccg gcgacaagaa
     1981 cggcgtatta tttaatgagc tggatgccag gcgactggtc agcatacgta aatcacgttc
     2041 agcttgctga tcaaccgcat ttttcagcca ggtgaggcgg gtagatttga catcatcgag
     2101 ttcttcagca gaaaaaccat gctgatcaat agttgccaac tctgccatta atgcattcgc
     2161 agcatcctgc atattatcgt ctcgtgcatt tacacggaaa aacagcgact gataatcggg
     2221 tgcaatttta acgctgcgcg cagtgccgcc agaaatagtc ttcaactcgc ccgactgtat
     2281 gcgttcctgc agacgctgat tgaacagctg aaccaacatg ctccattcag cttgttcgat
     2341 aaagctttgc tcatcgctca cttgcaccat tggcaggcga taatagagtg cgatgccgtt
     2401 cacccggttt tctttatcat taatgatatt aaagcgcagg tggttttcgg cttttgtcgg
     2461 ccagacgcga ttttcagctg ctttgttagc cggaagctta cttaaattat ccttaatcag
     2521 cgccagcgct tctttactgt caatatcgcc gaccacgata aaagtcatat tatttggttg
     2581 ataccagcgt tgataaaatt ggcgcaattg tgccggtgtg accgtggcga cagtatccat
     2641 caggccgata ggttcacgct ctaaattacg ggtatttgcc agcaggaaag ggcggcgcgc
     2701 ctgagaggtg cgccatttcg catcctgatg ggcacgccat tcctcagtaa ttacgccgcg
     2761 ttcagcgtct acttcgagtt tatcaaaggt tgcggcatta ccccattcac tgaatattgc
     2821 catcacttgt tgcagatttt gtttctgcgt agtcggcaaa cttacctgat acaccgtttc
     2881 gtcatagctg gtataggcat taacatcgcg accaaagcgc aggcccattg actcaaatgt
     2941 ttcgatgact ttattacccg gccatgtttt tgtgccgtta aacatcatat gttctacaaa
     3001 atgagccacg ccgcgctcat tgtcttcttc ctgcaatgaa ccggtatgaa tttgcagcca
     3061 taaatttacc tgatcctttg gttgagcatg cggataaatc atatatcgca agccattgtc
     3121 cagttgcccg gtaattaact tttcatcctg cggtaaggcg gcggcaatca gtcgcccggg
     3181 gagcaacaga gttgccacta acgtcagtaa gaaacagagg tttctcataa ttatctccat
     3241 gcgaaaaccg ggcgaattta cccggtaaag taaaatccga actattaaaa tttcatgctg
     3301 acatcaagcc agaaagtacg gccactggcg taggtcgcca cgccggtatt cgttgtatca
     3361 acagcggttt tgctatccag tacattgaga atatcggcac tgatcgtcag gttttgttgt
     3421 ttcagaaatt gtggcgtcca ggacaagcgg gtgtcccagg tcaggctgct atccagtttc
     3481 tcgtcaacat actgcttgta atctgaatat tcgctgatgt attgcgcatt cgtcttaccc
     3541 aggataatgc gagctttacg cgcttcttgc caggtcagcg tattcgccca caccaacccg
     3601 ctcggttgat gcgtgaaatc catgtttaag gagatcttta atggattatt aaaatccgcc
     3661 actggaacgc tatcgtaaga gaccagatta ccgttataaa ccacccggtt atcaccggta
     3721 ttgctctcct cataaccatt gtttaacgac aagttgccct tgctcttgat atagctaaag
     3781 acaatttgcg ggttaatatc tacctggctg atatgcaggg gttcggccag ttcaaaactg
     3841 aggttaaacg aatgtgtttt ggttttgcca tcgttgttat attcagtaat ggtggtttta
     3901 gtcgcgctgt cggtacgact gcttttgctg atttgatcat gcgcttcacg gtaaacatag
     3961 tttgcacgcg caataacgtt cttaccgatt ttctgctgca atcccattgc cagttcatcg
     4021 ttataaggcg ttttcaaatc ctgataacgc gtcagggttt tattgcctga taccgattcc
     4081 gtccagctat tgcggatatc acgtaatccc atatcaagaa tattcccgcc atagtaacgg
     4141 ttataacctg cagtaatcat tgaggtttga tcagcaaaaa tatcccattc cgtcataaag
     4201 cgcggggaga tattgtggtt tgacagatag ttatcatagt cataccgcac gccgggcatt
     4261 aacgacacat tccgccagct aatgcgatcc gccatataca gcgtgtagtt gtcaattccc
     4321 aggctgcctt tacctttatg gtaaatggta tggttagttt tctttccggc agcattaatc
     4381 acataggatt cagactggtt atggcgttca gtccatgcat cggaatagat gtattccgcg
     4441 ccgaagtagg gttgatgcga aacattaccc acggcgtatt tttgccagtc caggcgtgtt
     4501 ttgaaggtgt aattatctac agcctgggaa atgtgtccta atccgccacg ggtgcaacgc
     4561 cctgaaatat caccatatgt acatgaaagt tcggtgtacc atatgtcatg atcgtgacgg
     4621 gtataatcac taatatgatc ccaaccaacg gtagtacgca gtttggccca tgcgagctgc
     4681 gtatccatat cccatgccag accataggat ttattaccca tttcgcgatc agactccggg
     4741 aaggtgctgg tattataatc acggctggag ccggtatatt ttaaggttaa atcgtgggta
     4801 aagcggtcgc tggcaaacca ggtaaatttg ctcactgcgg tatcgataac gtttttatac
     4861 tgtgcccgac cggcgacaat gccgtcattc gaaacataat ccgcgcgggt gatatcagac
     4921 tggcggcgcg ataaaccggc ggtaacgccg aagttatcag cgagttcctg attaaacgcc
     4981 aaagtataaa agttcttttt aaaatctgga gagtaataag tacttcctga agaaccttga
     5041 ttaaatgcgc tcttgttatt ctcatcgata tgcgatgtta accagtccga acgcgtagtg
     5101 cgataaccca gtttcacgct gctatcatca gcgttgaagc gtttgatctt tgcatcaatt
     5161 accccgccat tgaagcgacc gaattcaact ggcacaaaac tgtcataaag cgtcacattg
     5221 tccagtaagc tgacatcaag ataatacccc tgtgacatcc cgctaatatt ggttgcacta
     5281 ctggcatcgg actcattcgc tgggttcagg ttattcgttg cactaatacc gtcaatcaaa
     5341 taggcattct ggtagggcga cgcaccgtga atagagattt tctccgggcg aatatctccc
     5401 tggttcaatg aggtactttg cgttgaatcc atgcgtaccg cagggttggt tctcagcaga
     5461 tcgctgatat tgccattccc ggtcggtaaa cgctcgatgc tttcactggt gtaatgcgtg
     5521 ttgccgttta cgggtacagg gacaggagca tagaccgtca tctcttcaaa aacatgcatg
     5581 tacatatttt tgtcatcgac ggcctgcgcc acatcagcgc cacataaaat gacgccagga
     5641 ataagaactc gcttcattta tttttatctc cgtacagcag ggcttttatt gtttttataa
     5701 aaccgcgcta atgtcacaaa tatcatcagc caggttccag acaccgggtt gatgtgtaac
     5761 cataatgacg ccgcttgtgg gtagtttttc acgcactaaa cgcagtaagc ggatagcctc
     5821 ttgttcctca agatgagagg tagtttcgtc aagaaatatc catttcggac gtcgtaaaat
     5881 taatcgtgcc agggcgatac gttgtttttc gccgctggaa agaatatcgc cccagcgatc
     5941 gtggtcatga atacgcgcag ccaatttccc cagaccaacc tgatgcagta cttcgctcaa
     6001 cgatttatcg tctacggaca ggggaagtgc tttacaaatt atctctttca gtaagccggt
     6061 tttgattaac ggtgtttgtg acacatacca actgtcagca ggagaagaaa tatcaccttt
     6121 aaaccacggc cagcagtggg ataatgtttt aagcagtgtg gtttttcccg cacctgagta
     6181 acctttcagt aatagccatt tgcctggcga aacatgaaag tttaggttct ctaatatgat
     6241 cttattatca ggagtacgaa tactcgcgtc agccacttgt accgcatgtt ggcaattttt
     6301 aggcttattc gtagggcgct gttcggtgag ttgatggaac tcatacaagc gatcgataac
     6361 cgcagccagt tcagcaagtt catcatattt ataaataaac cagcttaaat tgttcgatac
     6421 cagcataaat gcctggcgcg atttcatcag tccgcccaga ttgatctgtc cgctaataaa
     6481 ctgcggcaat aacagaaagt agggaagaac gctaagcgag cgcgaataaa tattctgcca
     6541 gtaatcaagc caccgctgac gattcattaa acgatgccag ttatctttaa tggtatgaaa
     6601 attgtcgctc aactcctggc gttgtagaga ctcagcattg cttagcgcaa tcagttcagc
     6661 ctgcttatta tgctgcacaa gattagttcg aaatgtcgct tcgctacgtt gtttttccac
     6721 attaagcgga cgaatacgtt taccaacctt atgagtaaat aaagttccac cgatcacaat
     6781 gagcacgacg gtatagacca tatacccctg gatattccat tctgttccac caacagtgaa
     6841 tgagagcgta cccgcgcttt gccaaagaat aacggtaaag gtgatcaaca tgctaagtga
     6901 ctggatgaag ccaaatgaaa gactgagcgt tttgctgatc agtaagagaa tgtcttcagc
     6961 gatacgttgg tcagggttat ctgtattttt atgcttaccg tagatctgcg tgaagtagta
     7021 gtttttatct gcaaaccagc gattaaggta gtaatctgtt aaccattcgc gccagcgaat
     7081 agtcagtagt ttaattagcc atgttttgtt tacggatatt aatacaaaga tccccagcag
     7141 ggcagggaac caaaggacaa gctgccaaag cttatcggtt tctttctggc ttagcgcatt
     7201 gaaaaaatca ttattccagt cgtttaacca gacctgaatt ttaaccacgc caaggatcat
     7261 ggcgaggata ataatgatta acagcactga agttttatta ttcttacgca gccaaaaggg
     7321 cttcagcaga cagagatact ttgctatcaa catacgaaac gtaatgggaa tggttatcat
     7381 tagcgaaaat tgattgtgcc atccaatgat tatctatggc aatgatttac tgatgaaatt
     7441 tatttaagtg tcatttaaca ttatgaaata taaagaaatt atttattttc aatggttacg
     7501 gttatgctat tgataatcaa gggtattgct aatgccctga cgcatgccac gtaagccaga
     7561 attttcgcaa gggaagatga tgatgacgaa acacccaaca gtaatttatg tcgggtgtct
     7621 tgttaaggcc ataagaacga agctaagact ggagcaaaga gggagcgtta ttaattattc
     7681 tccatttgtc ttgcaacatc catgatgtgg taaagcggta ctctgttctt agctaactcc
     7741 accatggcgt tcatataagg taccatggtt gaaaaaagga ctttataact ttcgcaaaaa
     7801 taagaaacag tctcattgtt tactttagta atacgatgct taggacaacc gccattgcag
     7861 ataggtttat atgcacattg ctgacatttc gctgaaatcc gttttttttg cgctgtcagt
     7921 tgtacactgt tcatcgtttt gagttcagat ttattaatgt ttccaatttt gtactgtgga
     7981 tagacaaaat ggtcgcattc gtaaatgtct ccattacttt caacaaccag attatccttg
     8041 caggactcct ggaaaataca actggtatgc ccattcccca aaaaacggct gacaaagctt
     8101 tcaaactgac ggatgaaaat ttcacccaca tcgtttttaa cccattgcat aaaaatggtc
     8161 gacataaact tgccataagc cgtaggaggc acagaaaaat caatgatacg gaatgtgttc
     8221 tcactatgac cactgaaatc aatattcggc gtcccggttt cgagcaattc gataaattgc
     8281 atatgtttac tgccgataga ttttaaaaaa tgataaacct caagagggta atggacatta
     8341 acgttattaa tgacggttaa cgtattaaac tctacttgat atgatttcag acgctcgatg
     8401 gctgctatca cttttgcaaa agtaccgtta ccagaattac tgcgtctgta acggtcatgt
     8461 agctcctggg gtccatcgat cgagatacca accagaaact catgttcttt gagaaaggca
     8521 caccattcat tactcaataa aatgccattc gtttgtaatg cattaaaaat acgtttttgg
     8581 cctgcatagc gttgttgata gtgaataact ttacggaaaa aatccaggcc agccagagtg
     8641 ggttcaccgc cttgccaggt aaaatagacc tgattgccag acgctgcgat atattgtttg
     8701 atgaactctt tcagagtgct gtcatccatc catttttcat gagtaaactg cgactctttt
     8761 tcaaggtaaa aacagtaatc acatttgaga ttacattgaa aactggaggg cttggctgta
     8821 acgtgcatcg ctatctcgct caataaggcg gcggaaaatt ccgccgcatg aaggtttagt
     8881 tatttcgctt cgcttagtgc tttcttgata ttgttaaact tctcctgatt tacttcgcta
     8941 agcggtggct ggctgctgtc gataaactct cttaccacgc cttgcatctc tttaacgacc
     9001 tgcggattgg cggcggcaag gttatctttt tgctgtagat ccgtcagttt gtatagacct
     9061 aactgattgt tttctactgt atagacaagc gaataatcgt tatttctcac cgtataagag
     9121 aattggctta agtcctcggt gttgggatta tgcgggtaat cgtctgactg atggcggaca
     9181 aatttgtggt aattatccca gaatggaata ttttcctcgt caaaccagtg agaataagag
     9241 gttatccagg tcagattttt atgtggctcg ccttgtttct tatcttgcaa ccacggcagc
     9301 aaggaaacgc catccagctt aaggtctttt ggaatgctga tatcggctgc atcaagagct
     9361 gtcgggtaga aatccattgc ggaaatcagc ttgtcataat taccgggttg aagttttcct
     9421 ttccaccaca taaacattgg ggtgtgagta ccgccaggat aggtctgact cttatagcct
     9481 ttttgcgccc cgttcagcgg cagaggacca tcgataaccg caccattatc ggaggtaaag
     9541 agaataatag tattgtcata ctgtccgttt ttcttcagtt gttcgagaat gcgttttaca
     9601 ccctgatcaa cagaataaac ggaagcgtag tagttatctg ctgtttgact accagtatta
     9661 aattgcttct gatattgctc cggtgcagga ttatcatttg gcaggtgcgg agcattataa
     9721 gccaggtaaa gcataaaagg ctggtcaagt gttttggcac gatcaacaac gccaattgcc
     9781 tcatcggtta actgatcgct gatataacct tttgcgggga cgcgttcacg gtttttgaac
     9841 agtgaagggg agttgtaata tgccgttcct gcagcgtgga atcccataaa gtaatcaaag
     9901 ccacggtttt gaggttgcca ttcttccgca gaaaatgtgg tgaagttgtc atgatagtca
     9961 cgcgtttgtt tatcttccgg tactggcaca ttactgattt ttgacaagtg ccatttacct
    10021 actgctgcag tgtaataacc atgattctgg aataattcag gcaagaaagt ttctgttagc
    10081 ggaataccat cctgagcatc ggtattggaa tagacaccaa agcgggcggg cgctcgaccg
    10141 gtcattattg cggcgcggga ggggccggaa acaccgtgcg ccacatagcc gttagtaaaa
    10201 cgtacgcctt catccattaa tgaaaggagc gtcggcgttg atttttgcgc agcttcaatg
    10261 gctttatcta tccctatttt gtatgtatcg acaacatcac gattttccat tgtttttggg
    10321 tcaaaagatc ccttatcaaa aggaagttgt ccataaccaa gatcatccat ggtcagtacg
    10381 ataatatttg gctttccttt ggtactgtat tcagtcggcg taaagtctga gaaagcaacg
    10441 tttgttttgg ttgctttcag ctttacatca tctgccgcat gagcagcaaa tgcagccata
    10501 ccagatgcca gtatcaaaga tatcgaggta cttacgacac ttttctttaa tgcagacttc
    10561 ataaatgttc ctcttcttat tgtacttaat tccaaagtaa ttttacgaag agaaataagt
    10621 gaatgtaagt gaagttagtc acataaagag atagcagatt tagctaaaaa aagggaaata
    10681 acagtccata aagcgttgac attactttct gttctactaa gtaatttctc gatgataaac
    10741 aactaatcca ttgatattta ataaattatt gtattttact gacaaaatgc cgaattgaga
    10801 tcataaataa tcatgcaacg ggttatgcaa atgcataaat atgtgatgaa tgtcacttat
    10861 ttatttcaat aaatatatcg cctaaaaaca acgcggggca ggaaatggct gccccattta
    10921 attcttacgc agcgtgtgtg gttgactact cgttagcaaa taatcaaata gctaaagcac
    10981 tcatcgtgtt gtccgtgttc ataccggtgt gcctttgaca acggtactcc ttagaaactc
    11041 tcttcggact gttgccgaaa tgtttgcgga acgcataaat aaaataagat gtactggcat
    11101 aaccacattg ttcggcaatt ttattgactg aaccttctac gcgtatcaaa ttttttgcgt
    11161 gctgcattct ggcatctaaa agaatctgtg agaatgtcgt ttgctcttgc ttaagttttt
    11221 tcttcaacag gctttcacta atgtacaggc agtcacaaat atctttcagc ttccatgggt
    11281 gcgccagctt catgttgaca atatttctca ctttcccgga aacggatagc acaccgttag
    11341 ttaatagtgt aatgaaacct ttacatgcgg caaaaataga caggcaagaa aaaagcaaaa
    11401 gttctgaaaa attatgatga ttgcgttctt ccgagttgag ataagcaatc atttcattaa
    11461 gcaatccagt cggtacattt gaagcacgta aaaacttcgc aggaacaggc accgtcactg
    11521 tctggatgtt agtgcattgt agatattttt taatggtatt ttcattaaat ccgatttctt
    11581 tgaaatgccg ctcataaggt tcaaaatgcg tacgaacagc gttatctacc atcaggatct
    11641 cgccgtcaga gaaggcgtaa tctttatcta aaatgttagc gttgaaggca tgatgaataa
    11701 atataacaga acaaacgagc gacattttat ctccttaaaa caataaagtt tttatcgata
    11761 cttttttaac atttcatatt tataatttgc tgtttatttt cagtcttgca aactattgat
    11821 aatgaaatgt gtaagatccc ttgcaatgcg acccatttct gaaataccca gctgatcaat
    11881 tgccgaaaaa atggctatca atgtaagggt cagtaataag acaaccacaa ttacagacat
    11941 gatcctttta tagttgtctc tttgcgtgat tttatttttc actatggtcg catgcatttc
    12001 aaatgtgttt attaaacgaa tgacgttaag catccttaat cagtatgctt aatataacag
    12061 gctggagaat agaggaaata ggactggcgc ttcagtactg agcggagttt cttacagctg
    12121 taggcggagg tatatatctt ataacgagca gtttaattta gcacatgtga gtaaattgcg
    12181 cgacaatggt caatgaggaa ttcttctgct gtgcggatga cagcagaaga aatgaggagg
    12241 cgaattaatt tgatggttct aattcaaccg gaatactttt atagccagga atgccactta
    12301 atggatcgtg gttatcaagt gttagcatgt gattcgattc tggaaaatag gtcaccagtg
    12361 agcggtcagc catagggtaa atgaccactt ttaatctatc catgcggcgt gagcttcgct
    12421 taccgtctgg cgtaagcgca ataagattaa ctctttcgcc gttttttaca cggcaaattt
    12481 tagcttgttt agcactcata aagaccacat ctcgttgacc gaatacccct cgatagcgat
    12541 catccatacc ataaatcgtc gtgttgtact gatcgtggct gcgtactgtc gccatgacca
    12601 gcttactgtt aaacgctgaa gagggatctt ctaacagccc tttgctggta atgaaattag
    12661 ccttacctga cggcgtcatc cagcgccttt cagcagctgc atttatcagg tgaaaaccac
    12721 cgggatgacg gatgcgctgg ttatagtcgg cgaactctgg cagcacagct tcaatgtcat
    12781 tgcgaatgcg atcataatct tccaccagat actcccaggc taccacgctc tggggtagtg
    12841 ctgcctgcgc gattcccgcg accactgcac actctgattt cagcattaca ccggcgggtt
    12901 ttaacacgcc acgcgaggca tgaatcatcg acattgaatc ctcaacggtt accgcctgcg
    12961 caccgctttt ttgcatgtca atctcgctgc gtcccagaac cggcagaata tagctatgcc
    13021 gtgcggtcag cagatgagag cggttaagct tagtggctac gtgtaccgtc agatctaagt
    13081 gtgttaacgg tacagcgctc gcttcccgat ctggcattgc cagagcaaag ttgcccccca
    13141 tgcagatcaa tgctcgagcc tgccccgtac atattgcttg catgctggca attgcagcat
    13201 gtccaggtgc atgaggtggg atgaaaccat agcgctcacc caggcgagcc agaaactctg
    13261 cagacggttt ctcggtgata ccgacggttc ggtcgccctg tacattagag tgacctcgta
    13321 gtgggcagat acccgcacca ggcttgccaa tgttaccttt catcaacagc agattgacca
    13381 gttgctgtac attctgggta ccatgttcgt gctgagtgat ccccattccg taacagataa
    13441 tggtgcgttc ggcagcggca tatgcatcag ccagttcggc gatttgagtc tgacttaatc
    13501 cagaaatacg ttcgatatct ttccactcgg aattgagcac gtcacggcgt agctcgtcaa
    13561 agccgacggt atgcgtttga ataaattcgt catccagcaa tgagggccga cctgcggcgc
    13621 ttgcagcatc atcgcgctca attaacaggc gcatcatccc cttgagcaac gccatatcac
    13681 caccaatgcg cacgttatag taggcactgg ccaactgagt ctcagagttc gtcagcattt
    13741 caaacgggtt ttgcggtgcg gtaaatcgct ccaggccacg ttcctgtaga ggattgatgg
    13801 cgatcatttt cgctccccgt ttcactaaag cgcgcaacga agtcaacatg cgagggtggt
    13861 ttgtaccagg gttatgccca atgcaaatga ctaaatcgca tttctcaaag tcttccagca
    13921 acacggtccc tttacctaca ccgatactcg ctgccaaacc cacactggtc ggttcatggc
    13981 acatgttgga gcagtcgggg aagttattgc tcccgtattc acgagcaaaa agctgataaa
    14041 gaaaggcagc ttcattggaa gtgcggcccg aagtatagaa ttcaacctga ttgggatcac
    14101 tatagctttg aaggcgttcg ccaatttcgt cgaaagcttg ttgccagctt aatggcttgt
    14161 aacagtcgct gacggcatca tatttcaaag gctgggtgag acgccccgca gcctcaagct
    14221 cgtgatctcc ccaggtaagt aatgattgaa ccgtattctc agcaaagaaa gaggcgttta
    14281 cctgcttatc cgtgacttcc caggcgattg cttttgcgcc gttttcacaa atgtcgaatg
    14341 acgcactgtg cttaggatct ggccatgcac aacccggaca gtcaaagccc tctggcttat
    14401 tcatgtcaaa catggcaata acatcctggc gtatatccat ctgcttacgt actgcattcg
    14461 ctacggattt aacagcaccc caaccaccgg cagccccctg gtaggattca atttttttct
    14521 tcatctactt atcctgtgtg gaaatgaatt attaggattt gaaatagcga tgatgcacag
    14581 tatatacaaa gggattatcg tcaatatgga tatataagag taggggacta ctctctttct
    14641 atttttaatc tattatcgtt aaataataga atggtataaa tacgataagt taagcatcat
    14701 ctctcatagt gtttaaatta acatgttttt caatataaaa aataagactc tggcttcaat
    14761 tgtgcgcgga ttttcttaca ggtgtaggat aatagccttc acaaaataat tatccgcgca
    14821 attttcgcga atggagcgca tagactaagg tcaaaatcac aattattcat agataaaagt
    14881 gacaccaatg actgactgga cggttccggc tgaaactttg tttcctgttt gtccataggt
    14941 tgcgcttaaa cccagaggta ctttgctttt atttactgtc cccagggaga cgttttctcc
    15001 cgtagccagt atttttccat tacgcatcag agagacaccc actcccgatg cttttgttgc
    15061 atctggtgct gtgttagcaa acacttgtct ggcactgtca gtggtagcac ccgaaagata
    15121 aaatgagagc ttttgttcac ttgagcaata tacgccaagg ggtatttctg cgcttccagg
    15181 aaagtccggg agattcacgt tgacattacg actatcaacg gtgcagccgc ctgttggcat
    15241 aacaacactg ttatttgaaa taatattcca cgtaaaatta cgcggatttc cgcttcctaa
    15301 cgtggcaatt ttatacatat gaatacgggc aataacttcc ccggctttta tgacaactcc
    15361 accggccgca ccgactgggg taatatagag ttttaacggt aatggcatcg gtgtcttatc
    15421 accaatatct aatacattag tatttgtcgt caacgggaag gggtaggtca cattattcca
    15481 gtacagcgat cctttatatg actgtagaga gcctgcgaaa gctgagcctt gtaccaggtt
    15541 tatatgatca gtgtcgtacc agccgccgta atcattccag caacttatat gctgagacaa
    15601 gtctacaacc agattctggc ccggttgtat tacagggtcg agattaacat aaacagaggt
    15661 tgtacctgct ccaatacttg acccgccatc gacattacaa ctgaaggcaa aaactttgcc
    15721 tgccattaat agaagataaa taccgaacag aagcttcata cttattgttt tacccatgat
    15781 atatcctaag gttaaaaaat tgatttaaaa aggaggctaa gaaaatcgaa tcgacgttat
    15841 tgccaggtgt agatcacatt gattagcgcc tcgatcgttc cctggcttgc gtttccattc
    15901 accgtgatag ctcttgcctt aagtggaaac tgtgcattac gagtgatctc atcaacaata
    15961 accgttttgc tatcgccatt ttttaacgtc gcatcctggt catcccttag ctctatctga
    16021 atattttcgg cagtaccttc atttttgtaa taacccgtat tgtcagttga acctgtcacg
    16081 attgccgtca ctgcacttgt ttcaaccgga caatcggtta atgacaaagt aatattgtgc
    16141 cagcctgatg cagaaccagg ttgttgcaga ttgcgcgtat aaagatcccc gagattaacg
    16201 ttagcttctt tggtttgaat agtgcagggt ttagcgacta cccgaccatt aacagtgata
    16261 gtgacatcgg cggcctggag agttgctgtg aataacgcgc aaaacgtagc taacaggaac
    16321 ctcttgtgaa gtcttttcat atctgactct gattattgaa attcaagggt aaaggttgcc
    16381 gaagccctca ccagtcccgg attgacggac ttctgagttg acttcagacg agcggagtaa
    16441 ggcaaaatat tgttctgttc tggtaccagt gggatccact gcatcccggc atgaaggtca
    16501 ttcaatttca ccggacgcat atttccgtcc agtatttcta tacccaaacc ggaggctgta
    16561 ttgcttcctt catccagttt caacagagta ttattttctg catcctcaat accgttaaat
    16621 gcaacccgaa cccccgttgt ccctttactg cattcactta acgtaatctg aaaagggacg
    16681 gctggacttg tgctaccggt cgttggaaat tgtctggcac tgtttttttg gagatctacg
    16741 gtaaaattaa gcgaatccga tgagactgtg cagccataat cgaggacgcg cccgctaatt
    16801 ttaataacgc tatctgcaga taaagcagaa taggtggtta accccagaca taaaccgagg
    16861 aaaataatgt tattgtattt cataatctat tgttccttag cgacagattg ctgtctgctg
    16921 gttcagtaag gtaccaggag aaacttcagg aagcttgtac tcgacaatac agtttgagtt
    16981 tttatctttg ccccatgaaa cctgtaattt ccctgactgt ggaagtccag tcagataaac
    17041 ctgaccgttt tccgcgacaa tgctgccatt tttattctct ccatgtgtga caattgcacc
    17101 gaatggaacg ctcttattac cgtacttcaa cgtcattaat actttcccgc cgatttgtgc
    17161 attaaatgtt gctctggcaa tagcaccgtg agttgggatg acagtgacca cggtttcatc
    17221 cagttcaaca ttatctgcaa gggaattcgc gttaagagcg acacgatttt ctctatattc
    17281 tgtcgcaaat ggtaatatgg catagccacg ccagtcggta tgaattccgg tctggttctc
    17341 tattttgaca ttatcagcgc caggagcctt aaccagaacc attgtgtcgc ccagcggctg
    17401 tccaaaggtg atgccatcag catgagcaat aatgccacca ttcattccgt aataaatctg
    17461 gctgctgtca ccactccgac tgtaaccgac attcgtatta ccgtaagctc cacgataatt
    17521 aagagaactg taaccactgg tgccagacga tgtattaccc ccgtgggtgt taccgacctg
    17581 aacactataa ttcaggttat tatccggcag cagagtgcca taaactcccg atagattggt
    17641 catgccgcct ttcaaatcgt ttgacatact gtaactggcg tttgagttac gaaatgccga
    17701 ctgactgtct gtacgcatcc aatgactgaa gggaacatta agcgtaaaag caagtaaatg
    17761 atcccgatcg ttttgccata tattattgga atagctgtaa ttcagcgaag tcgtaatatc
    17821 accaaacggc acatttaatc caaatgatat ttgctggtcg ctgcgtgacg tgttccagta
    17881 actttggcga ctggcactga aaaatgtcgt accgtaattt ccaagctgct gagagatgct
    17941 tatttgttcc tgaccacgct tactgtagaa cagattaaaa taatcaataa attgtgtctg
    18001 ctcattgctg tctccggtag gaggcttgac ggtgtaacca ctcattcgac tgtaggcact
    18061 gtcggataag ttataaaaac cttgcgtaga atagcgatat cctgcgacct ggatattggt
    18121 tcccgtctgg tagaaggatt tgctgtagac ggatttaatc gattgcccgc tgtgacgggt
    18181 gccatctgca agtgtcgtat tggcctgcgt gatatcgaaa gaaaaggcac caaaaagtcc
    18241 taaatcttta ccaataccaa ggttgaaggc ttgataatct tctgcaattt gcattccgcc
    18301 ataaggtgtc cagtttcctt ccagtccatg catcaagcta ccttgtatga acttggggga
    18361 gctttgcagg ttatttccac tacgatattc ccccatggca agcgcataac gcgtatatcc
    18421 agcacgttgg agaaccggaa cagacgaata aggaacatat aatgtctgaa tactgccgtc
    18481 tgcctctttt atggttactt gcagatcacc accattggcc gcagagttga tatcatcaat
    18541 agtaaaaggg ccgggtggga cagtagtctg ataaacatcg tatccattct gttttacact
    18601 cacttgtgcg gtgccgcggg caataccatg aatcaccgga gcgaaaccat gttggctatc
    18661 gggcaacatc gcttctgttg aatttatttt gaggccacga aagttcacac tatcaaaaat
    18721 atcgccatcg gtataactat cacctaccgt taagcgtgag cgtaagggaa taatgtcacg
    18781 ttcagccgac gtattgatat gctgccattt attgctgtca gatgaattgc tactcccact
    18841 gttataactc cacgttgagt tatcgcgcag tcgccaacta ccaatgttga tgccactctg
    18901 taaattcaaa tatgcatagt tggattttcc tgcattatgg ttactacgat tattaataga
    18961 attaccatta aaactatagt ttagaagccc agcatttatt ccttcttccc acagatcagg
    19021 agaaacatag ccgcgagcca ttctgccgac ataaatttgc ggaacggaga gtgacagatg
    19081 ttgctgacca acatcaaact cagttgatgc gtcggccaaa cgatcctgaa gtggtgtgca
    19141 tgcgctgttg tccggcacat gtttttctgc caaatgttct ttattgtcta ataacgctga
    19201 tttttttatg ccaagactga caagaagatc tgtactcagg catgggatga gatctgcgtt
    19261 attatcatct gcaataaaag taatatcccg actagtcata aattcgtcgt tcaaataaat
    19321 gtcgacccga tatgtaccag gtggtgcctc gcgtccctta gtaaaggcgg ataaatcgac
    19381 agattcagca agatcattgc ttaaaaaccg tggattaaaa taatactctg cgtcaacggg
    19441 aaaaaccgca gttgctaagc caagggcaaa ggtaagccgt gcaaagcgag gcagtattaa
    19501 cacctgatgc atacgatatg ccttaaatgc ggcatgaaag gctgtcattt ttaattccga
    19561 ttgagtttat tattaattag gtcactggct tattgcatgg ttgccttaat acgcggagtt
    19621 aatgcaccgt aatcattgat cgtctgaaaa ctgatatccc cggttgcagc atgcgggata
    19681 tccaccgata cttcaccttt tggcggcacc atggtgtttg ggagattgct atttcctgct
    19741 ttcatatttg ttacggtaat gaagtagggc gttggattaa taagagtgag tttgctgccg
    19801 ctgcgacgaa accgaagcat tgcaggcgct tcttcaggtg ccattgccag gttcgtgggg
    19861 cgataaaaca tttttatgcg gctaataatt gccagctgta atgtattctc attcttctga
    19921 tctttttcca tagcaggaat agctttaaca ttaacccaga acaggctttc acggtcgcct
    19981 ggtaactgat gattagtggc gttgattatc cgcagcgtat tctctttttt accttgcatg
    20041 gaaaaaagcg gtggggtaat gacaaactgg gtgtcttttt ggtcacctgc attttctatc
    20101 catgactgaa tgagatagac actggcagga tcattattcg tgacaggcaa taaaacctgt
    20161 ttctggtttg ccggataaat tacccgcgtc gcacccagtg cgacagctgc gttggcaatg
    20221 gtgctatggc aagcaaagag caataaaagt aacactgtcg ccataaagga aatagagtag
    20281 ggggtacgcg tagtttgcat ctgatgcatt ctccatgtgt taagaggata agcggggaaa
    20341 ccgttacatc agacaatcgg taaatgttgc taatacattg gcgagtcgcc gggtttccag
    20401 acttaatgcg tttgaaataa atgttggttc tcttttaata ggaaaacctt gctacaaagt
    20461 aaatttttta aataacagta aatttaacta ctgttgttta ttgatcgttt agatattatg
    20521 tgatttttca tagcgctcca aatacaaacc actcctcatt aataatgaga ttcacatttc
    20581 tgagttgcta gggtgtgcgt agccatgcca cacacaccct gtttcaattt cagatgacgt
    20641 atagccacct taagattgaa ttatagatat tgaactttga aggtcgcatc tgcgtttgca
    20701 gtaccagcta ctgactgccc gagagcaata tatcgagcct ggaacggcaa tatattcgtg
    20761 ccatcaatca agtcagtttt agcgctaaaa gtggcaccat caagtattaa tacctccccc
    20821 gtacggtcca aaatttgaat accgacattt tgggcagagc ctgccgctga actctgcagc
    20881 gctaatgtgt cgtcattact ggtgacagta gtacccagga atgcaacagc tgcattactg
    20941 gaaacattgg tatcacaatc attcagcttg atattgaagc caacggcgga gctgaggtca
    21001 cccgctttag ccagacgaga agaacggacc tgacccagct caaccgtttg gttcattgat
    21061 tctgaatcaa tcgcacaagc ggcatttacc acttcacctt taaaatgaat aacaccgcca
    21121 ttaacagtgg tcgttactga ttcatcaccc tcggctgcgc ttaccgcagc agacgacatc
    21181 gccaacacag aaacgacaat cataccgaca tgtttgagtt tcataattct ccaaaattta
    21241 tatttaagta gctgaataaa gaaagttaac aattaagtca ttctgttatt gcttataaaa
    21301 caagaatcat tcttgatggg atattgccct gagaatatag tttccagggt tattacactt
    21361 cacgaagaac gataatatct ttaaattaca aggacaccta atctcaacaa gtttattgca
    21421 gcacttgttg tagagagtat ttgcaaagac attttatgtc gctaaataac attacgattt
    21481 cattatgcat gttatttata aacagcgact aaacagaata ttattgcgaa caagggaagt
    21541 gtcaggaaag gtcaagagcg accaatggtt tatatggcta taaatcatta gccatagtta
    21601 atgtttgcct ggtgtaacta acacggaatt gctttaaaca gataagtgca gggtttatta
    21661 aagctgaccg acagactttc ttaacctggt aatatctctc ttaggtgact ctccaaacat
    21721 ccgcgaatat tcccgactga aatgggataa gctttcgaaa ccgacagcat aggctgcagt
    21781 ggtgacatcg taatgttcat tcagcatcag tcgcctggct tcattgaggc gtagccattt
    21841 ctgataccgg agcggactca ttcccgcaag ctgtctgaag tgttgatgga aagtgggggt
    21901 gttcatttgc acgcggtttg ccagcctgac tcttcaacgc agggaagatg cggcaaacgg
    21961 caaacctgca taccagccag cctatatccg gtatgcacag gccaatcatg aaagaggaga
    22021 ggctttatgt tctggaaccc tgaagtaata acgacgactg gaaaatcgct tcgcatcact
    22081 caaattccat ttaactattc tgggtttgag ggatatacat aattgacttc agcaccagaa
    22141 acagcataac aaactgtttt aaatggatat taatcaaatg gcgattcaaa ctcacagtta
    22201 tgaactcagt tttggttcat aactgtgtta aacgctgtcc ataaatcact cgatgttacg
    22261 cttattggtt catgaactgt tcgttctaat gttgtgatgt gcgtcaggtt ttagtgttcg
    22321 gtaatgttac cttttactcg atgatcgatt gtttagcacc tctcccacaa ccggagcagc
    22381 ggtaaatgcg tttggccagc cagcatagaa ggcggcctgg gtaacgatct caccagcttc
    22441 ctcaacagaa agtccattat ccattgctct attgaggtga tagccaatct gcgcgctctg
    22501 tccggatgcg attaaggcgc tgaccgtaat caggcttctg tcgcggggtt tcagcgcggg
    22561 tctttgccag agatccagga agagtggatc agcagtgaat ttaaccaggc ctggagatat
    22621 tgggccaaca tttttttcca ctgtctctga acgctgcttt tctgcttcct gattgagagg
    22681 aagcaggtcc ggagaagctt caggaagtgc atctgccgtg acgccacgag tttcaaatac
    22741 tgctttggtc acgctgacgg ctgacattgc atttggccaa cctgagtaga acgccagatg
    22801 ggtaatgatt tctgataatt ccgctggcgt cacgccgcta tcgagagcga cgtcaatata
    22861 gtgcttaagc tcggcaggct ggttgcgggc aatcagcatg gcgacggtca ccacactacg
    22921 atccctcggt gagagggcgt cgcgtgtcca cagatcttca gtaatagctt cgcgaccaaa
    22981 gcgcgcgaga gccggtgaga cggaacgaat atcagcttcc gaaatcgctg acggttctat
    23041 tttcatcata gctttacctc ttgtttcctc agcattggca aaatcagaaa taacaagcga
    23101 catagccgct gctgcaagta cgcttttcat gttaatcctt actggtcaac gttgcggctc
    23161 ctgtttgatt ccgcctttgt tacgaagaaa taccggtttt accgggattg cgtccttcct
    23221 ggcgctcata tgcgccgttt gcctgaggta atccaggaag tgcagcgttt gtcggtgcag
    23281 ttgataatcc tcctcactgg cgtaaatctc atagaaatac cagcgttttg gagtctctga
    23341 atctgtaccg gcatacatgg ctaacacgcc tttctctact ttgagcgact cgaccatttc
    23401 aggaaggacg atgtttctaa attcgctctg aaattcaggc tttacttcga cgatcaccag
    23461 attattgata gttcgttcat tcggtatgat gtgtttgtcg cccagaaact gtggctccag
    23521 ggtaattttc ttcttcctgt caataatgtc aggtgcacga gcagcgaatg ctttgtatgg
    23581 ggcggcattg agatgtttgc gataagcacg ctcattttca tagagttcga acatgaatgc
    23641 ctgacctgaa ttgtcgctgc gttgcagggc atacatcgcc agtgtacctg cttcatgatc
    23701 aaccgaagcg gaaatcgtct gtctggccac gtctgcaaag tcgttgtccc gatcgggtct
    23761 gatgccgagc tcgaagatat tcatctccgg cttattcggc gcgtccggtt ttgttgcaaa
    23821 gacagggctg cttattagca tgcaaattaa aagcataaaa gtctttttca cgatgatctt
    23881 tttactgacg ttactgttta agacgttcga tcaactggtg ctggtaagaa cgtaaccgac
    23941 cgctactggc gctctcaaag acgagaggag gaaaagtgaa taagggctat cgcatccaga
    24001 cctgcttaac atctttcgtt tgcggcgtat tttgcgccat gccccccgtc agaacatgcg
    24061 gctggtaggt ttcttccaga aactgaattt cctccggact tagttgaaga tttactgcgt
    24121 ttaccgcgcc atcgacatga gctttttttg tagcccctac gacaggtgat gtgacttttg
    24181 tcagcagcca agcgagagat atttccgtca ttgacaccag atggcgttcg gccagttcgg
    24241 ctacacgctc aataatgctg cgatcctgtt cagccgtgct gtcatatttt ccacgcgcat
    24301 agtcatcctc tgtggctcgc cgtgtatagc cttctttacg agagaggcgg ccgctggcca
    24361 gcgcgctata gggggtcatt gcgatattat cctcagtgca caggccgaag agctcccgtt
    24421 catcttcgcg cataattaga ttgtagtggc tctgtacgga aacaaagggc gtcagccctt
    24481 cgcgttcagc aagggcgttc gccttcgcca gctgccaggc atagcagttt gaaataccaa
    24541 cagatcgtac tttgccagca gtaacagcgg tatgtaacgc ttcaagcacg tcgataatgg
    24601 gcgtgttgta atcccagatg tggtagatgt agaggtcgat atagtccatc cccagattct
    24661 tcaggctctg gtcaagggat tgagcgattg cctcttttcc gctgatccct ccggcaattt
    24721 gtgcgggggt tcgcggcaga aacttggtgg ccaggacgat atcatcgcgt ttcgccatct
    24781 cccgtaatgc ccggccaacg tatcgctcac tggagccatt ctggtaggct atagcggtat
    24841 cgtaaaagtt gatgcccttt tcaaggccat agcgaatgat gtcgcggctt gctgtttcgt
    24901 ccagcgtcca gctatgctga cccgtcgacg gatcgccaaa ccccatgcat cccatgcaga
    24961 tgcgggaaac aagtaagtca cttttaccga gccgggtata ctgcattgtt attctccctc
    25021 tggattattc acgacggaat tgagcagttc gacaagcctt gaagcgtgtt cccgggcaag
    25081 cgttttttgc tgcgcgattt tgtcggcatc ccggtcggca tagctgatac cgcaggtata
    25141 aaccggcgcc agcagctcaa gattgcacag ccttgccgtg gtttcgaacg gaataagata
    25201 ctcttcaatg gtatggccaa agaaactgtc gacggtatag agctcctctg gcgctcccgt
    25261 tgtaaaggag aggatcagct ttttaccgcc cagtttcgcc gttgagccat gcgcgaagcc
    25321 gtgaacaaag acctcgtcca gccatttttt cattaatcct ggcagcccat accaggaaaa
    25381 agggaactgc cagacaatca catcggccct gagcaggctt tcctgctccg ccgcgatgtt
    25441 gaatttgcca tccggataga gcctgtccag acgacgaatc tcaacatcgg gaagggcggt
    25501 ctccacttca tcaagaatag tggcattggc gacggaatgg gtaagctcag gatggcctga
    25561 aacaataagg atatttttca tttcaatgag ttaaatatat ttggtgaatg tttaacccag
    25621 tatagagtga caccattatt ttttttagag cagtaaagac tcattcactt ttagacaaaa
    25681 actaataatg gtcaactgtt ctcattgtac cggtcatcac gaacccgtga actacgccgt
    25741 gatgcacagt aaacgttctg cattgcgaaa tgcgatggcc tctttttcag ccttgttgac
    25801 gggcagactg tctataaagg tttttacacc gtccagggtc tgatagggat aatcgacgga
    25861 gaacaggatc ctttctgccc ccattaactc gtagataaac tggaagtgcg gcagtgtcag
    25921 catacctgac ggggtaacgt aaacgtgttg ctggaaggtt ctcgttagcg tgcgtgacag
    25981 acccgttgca gccagtggta ggctgtcatc aagacgctgg agccagaagg gtaacatctc
    26041 cccccagtgg ccactgatta cctgcaggtt gggatattta tcaaatgcgc ccgataacat
    26101 cagccgcaac aaatggatcc ccgcttcgtg atgccagccc caggcaaaca tagaaagcct
    26161 ggcgttgacc tctgcgctga agcccgtgaa gtaggcctgc tgtacgcttt taagcggtaa
    26221 cccgggatga agatagagtg gcacattcag tttattaaag cgggaaagca gggagtcggt
    26281 caggattatg accatcagcg acggttttcc cccagtcgcc aaggaaaggt gcctgctcaa
    26341 gcgcaacagg catacacgct cgccctaact cgctgtcgag ataattttct tcgaggcaaa
    26401 tgattttcat attctcacca ttcatacaaa gcagtaaaag tgatacagat tagaatcacc
    26461 cggaacagcc cgttcgtgtg tgtcattcat gacagtctgt tccggacggg ctttacacca
    26521 tcggtttttc ccaggcgcct cgtgtatcca ctctggtctc tgcggcgagt tgttccagca
    26581 ggatgatttc ctcatcgcgt aactggatgt ctgaagcgca ggcggcgtcc agaacgtgat
    26641 ggactttagt cgcaccaacg aggggaaggg tacctttcgc aatagcccat gcgatggcta
    26701 tctgtgccac gctggcattc ctttcggctc ccattttttt catggcggcg gttagtcttt
    26761 ctatctgcgg cagtactgcg ttataacttt cggcccggcc actcccggca ggcatgggat
    26821 gatttgaatc ataacgacca ctgagcgcgc cttgctccag gaccatgtaa gcaaagaacg
    26881 tgatgttatt ttgtcggcaa taatcaagga tcccggcctc ttcagaagcg cgatagagca
    26941 ggctgtagtg attttgtact gcactgaggg aataaccgga agcgttaagg atttcattgg
    27001 cgcgtctgat ttgtgccaga ttgtggttgg aaacacctac gcgttttacc ttaccgcttt
    27061 gtaaaagtgg gatcagtcct ggcgtccatt tttcgacgtc gaggggatta tggatccagt
    27121 agatatcaat cgcgtccacg cccaggcgcc caaggctggc ctcaagcata tcgctgacag
    27181 gctgcgcaga ctgcttgtcc gcaatctgcg gcgtgaattt ggtggataaa attatgttct
    27241 cgcggggaaa ctgacggact aatgctccca gcgcagtctc ggaactcccc ataccatata
    27301 ctgctgcggt atcccagaga ttgaggccct tactcatggc cgcagtgaac acttctgcca
    27361 tctgagtatc agaaagatga ttaccaaaga cggtgtcgcc gccggcaaag cctgtgcccc
    27421 aggaccatgt acctagtgcg actggaggta gtgttttcat attcagctcc tgaaaaaagg
    27481 ccggatcgtg gctgacttaa accggccaag taaggtgagt aacgatgaat taattaagct
    27541 gtccggcgga gtgcatttaa cagcgccaca ccaaccccat gtgctgaagc gggattctgc
    27601 cccgtgataa ggcgatcgtc ggtaaccacg ttttcagacc agtttggagc ttcatgataa
    27661 agcgcaccat gctcgcgaag tgcggtttca agcaggaagg gaaccacatc tgttgtgtga
    27721 acttcttcct cttccttgtt ggtaaaggcc gccacatttt tcccttttat caggtattcg
    27781 ccgctactga gttttgcgtc aaccaatgcc gcaggcccgt ggcagacggc tgagacaatc
    27841 ccatcacttt catacacttc gcggataata cgctgggcat cctgattatc acggaaatcc
    27901 cacatggtcc catgaccacc tgcaaagaaa actgcgctgt aatcggcacc tttcagctcg
    27961 gataacgcag gtgttgttgc caggcgctgc tgaaaatcgg tttcgttcca gtaccaggcg
    28021 ttaacagggt cgctgaggtc aaatccatcc accggtggct gccccccgcg aatactggct
    28081 attgttgttt ttattccggc atcttccagt actttcagcg gatgagtcag ctcggccagg
    28141 aaaaaaccgg ttggtacacc ggatgcgcct ttaatcggat ggctggaaac gacacatagc
    28201 actggtttat atgcaggttt catatcggtc ctcactcttt atttttaaat tcatcggcga
    28261 agagattttc ccagttaata aaggagccaa gcggtataca ctcgaagctt agtaagcctg
    28321 cttttgccaa cgggaattcg gccataacgt tcatcgcttc atcgacagag tcacattcaa
    28381 gaaaaatagc gacgccaggt ctgtcctgac ggaagtagat gtcgcggatg aagcctgatt
    28441 tatacaatcc ccaggcgtgc agcgcttcag cgttgaggtg cggagcatat ttctccagcg
    28501 atgctccagg ggcagggata tccagacata aaattcgcat catagtctcc tgaactcagg
    28561 tcgtagtaga aagagactaa agtataagag gcgtatttca ggaagattag agggtcattg
    28621 acttaagcat ttttagactg tacttaataa tcaggcgaga attgcttagg aaaacattat
    28681 gaataacgct ctttataacc agattcgcat cttccagagc attgcgcggg agggcaatat
    28741 ttcggcagcc gcaaggaaac tggaaattac gcctccctcc gtcagcaatg cactaaagct
    28801 gctggaagag tatatcggcc atccgctttt tgtgcgtacg acccgccgta tagagctgac
    28861 ggaaaccggt caattattgc tggaacagac cgctgcggcg gtggagtcgc tggaaaactc
    28921 acttgaaagc attcgcgacc agcatcagga gccctcaggg atggtgcgaa ttacgctctc
    28981 gcgttttgcc tacctgttaa ttcttaagcc tgcgatggca gaattctgtc agcaatatcc
    29041 gggtatacag ttagaaattt cggtctacga cggtaccgtg aatatcatca aagagcgttt
    29101 tgatctgggg atccgttttg gcgatattct tgagggaggg ggggtggccc gaccgttaat
    29161 gaaaccgttt cgtgaaggat tatatgcatc ctcagcgtat ctctcccgac atggaacgcc
    29221 tgaagtgccg gcagatctct gtcatcacca gctgattggt taccgtttta tcaccaacaa
    29281 ccgcatcctt ccgctgctcc tgaacgatcg cggtgagcag ttgaccgttg agatgccggg
    29341 gcagttaatc agcaacgata ttgacgttat ggcagacggg atccgtaacg ggctgggaat
    29401 tgggcgcttg tttgagcctg tctggcagtt acagccggac agggagcgct ttattccggt
    29461 gatggagagc tactggaaaa cctacccgcc agtgtatctc tattacccca aaaacgcggg
    29521 taaaacgaaa agagtgaagg ccctgattga ttttctgata tccactacgg ggcgataaag
    29581 acgataacgc ctcgttcggt atggagatgc atcgaatggc attgtgagtt ttttttcaca
    29641 gatgggcgta atcgtcagtg gattttacat acaaggatag cgcccacata ctgacatcat
    29701 taattaacgt tgggtgaggt ttaccggggg gaggggaaag cgaaccagga gatggaggtg
    29761 gccaatctgg tacgaaccgc cgcgtagctt gcacggcaaa agctgctgac tgattcatca
    29821 gttaagctac tgaaagagcg gaacacatga acttaagaac aaacccacca catttaacat
    29881 aatatacatt atgcgcacca aaattataga ggctcaatgc taatgttcta tttgtggctc
    29941 attggaggca ctcagtataa tccgggagca ctaccgtgat tttggcccca cgctggcgcg
    30001 ggaaaagctg gaggaagttc acggtctggt ccttggaaaa gaaaccatcc gacgtctcat
    30061 gataaacgcg ggactctgga tcccccgcag acaacgcgca ccgaaaattc atcagcctcg
    30121 ttaccggcgt ccttgtactg gcgagctgat acaaattgat ggctgcgacc accactgaat
    30181 acggtaaacc gatgatcctc tacagcgata aagccagcgt attcagagtc aataataagc
    30241 acgccaccac cggtctcggc gatacccagt ttgccagggc catgcgctgc cttaatatta
    30301 ctcctttgtg tgctgaaacc agccaggcaa aaggccgcgt tgaacgtgct catttgacac
    30361 ttcaggaccg actcgttaag gagctcaggc ttaaggggat ctgtacaata gaagcggcca
    30421 atgcatttgc agaagaatac atggcggatt ataaccggcg ctttgccaaa gaacccttgc
    30481 atgattttaa cgcgtaccgg ccgctggcat tagacgatga ccttgatgca gaatttacct
    30541 ggcgagagcc tcgccgggta tcaaaaaatc tgactgtaca atacgataaa atgctctatc
    30601 tgattgagga caacgagtac agtcgaagag ccatcggcaa gtatattgat gtctggcatt
    30661 atcctgacgg acataaggag ctgcgactaa atggcgtgct gctaccctac tccacctatg
    30721 accggctttc tgaggttgag cccgtcgcca ttgtagataa caagagactt ggccatgtgt
    30781 tggatgttgc ccgtcaggtc cagagaaaac gggacaataa tcgctcacca ggtgtagtca
    30841 cccgcgccga caaccagcgt atcgacgctg atatatccct ccgcgagcgt cgcagcagga
    30901 atatccgtat cgaaaatgag cgtaccgcca ttcatggcca gtccaccaat ggattgctct
    30961 cccacgttta ccgatgtggt attttcactg tcagactgca acatcgcgtg agtaagcgca
    31021 gcggtgttgt cgcgttccag cgtgaaggta ctatctttca gttgggcaac accggcgaat
    31081 tcagtccctg ttgcatgggt aaagccaaac atcttgtcgg aggatgacag ctgcactcgc
    31141 atcaagccat cgcctttgag cgcattggtc agcgtgtaat ctcctgcact gttcgttgat
    31201 gcgagaatgt cgagcgtacc ggggtttgca atggttactt cactcgaacc catgcccgtt
    31261 accccatctg ccgtatgaga cgcaatggtc aggaggccgt cattgaccag cgttttaccg
    31321 ctataagtat tccctgcatt gaacaccgta tggcctgcat catggatcac ttttccgtct
    31381 ttatcgtcac ctgtaatcag catatcgacc tggtagccgg catcactgtt attagtatga
    31441 ttaaagacaa aaacaccttc gccagagcca aactctattt tcgtcgcgtt ggtgataaat
    31501 ccggcatctg ccgccgcctc accgtgagct gcaccaatgt ttagcgttcc tacagcaggt
    31561 tcaaaaacac ctaagtaaac ttctccacct tcgacattca gcgtaccgtt attcgtcagt
    31621 gttaacgtac ctgtgccagt cgtccctaca ttcatattga atgtttcaag aagagagttc
    31681 tgcccatcca cccgcacgtc gcccttactt ttgtcattga gagctatctg tgtatcacgc
    31741 gctttaacta tacctccggt ggtaatattc agttcacccg cgcctaatac accgacctgt
    31801 agcaattggg cgttagtaga tgacgttttt aaattccaga gactatccgt tgaaatatta
    31861 actatcccct taccggaggc atttcctccg ataaatccat agcccgttga attaagagtg
    31921 gcaccattac tgacatttac gatgccgcta ccaaaattac cgacattaat acctacagta
    31981 tcgaacaatg atgtcgcatc catcaggttc agtgtgccga taccgcttgc tttatcgccc
    32041 aaaaaagtaa tattttgcga tacaaactta ccgccatcgc taatattgag gtttccgaca
    32101 cccgcaacac caatggtggt atagacattc ttattgacct cccacatccc cccccgtggt
    32161 gatgctgaca ttcccgacgc cggtttctcc ataaccgagt gagcttcctc cgttgctgac
    32221 aacaagcccc tcattactga tattcatttc gccgtggccg tcataaccaa gattagttcc
    32281 gagattagtg ataacggagt tcttatcctt aatagtaatg ttgcctgtgc ctgtttcttt
    32341 catccccgca gtgataattc ccgaatctac ttttccttca ctcgagacat taagttcacc
    32401 gtcgccagca tcaccgatat agatatagcg gaaagcttcg cccgttccga ggaaattcca
    32461 atgcccttta tcagtcacgt tgacgacacc gtgggaaccg tcctgaacgc ccaccaatga
    32521 atattctttg ttattaatca gtccattatt ggaaatattg actttgccat taccgaaata
    32581 tccattatag agtctgcgta ccgtgatgac agagtcttga tcctgcacat tcagggttcc
    32641 gataccggtg gcattgccac cgataatcgt attttcagcc gtaatcagcc caccctcgcg
    32701 aatagtcgct tccccagttc cttgattgcc aatttgaaat tcaattgagg aatcattatt
    32761 ttttattagc cactcgccac ccttttcaac gacaacctta ccattactgt tcgcctgata
    32821 tcctacaatg gcgacgattg aactcgtgac gtaaccctta tccgtaatat ttaatgagcc
    32881 tgtaccgtaa ctccctattt cgaataattc ggtcatcaga accgagtcct ctccctcaac
    32941 attgaccgtc ccgacgcctc ctgtcgaaga acctatcctt aaatatcctc cgtcaacgtg
    33001 ccccttctgt ttaatattca gcatccccgt tccggattgg cccacattta aaggccttgc
    33061 attatttccg ctatcataca atcgccaggt gccacccaaa acattaacgg tgcccttcga
    33121 gttctcatta ccaccaatga cgctggtagt aatagtggtt aaggaggcat taccgccagc
    33181 caaaatatta agcactccag tgccatacca cccaaccagg taggcatcat aagcaatatc
    33241 agtattaata ttggtaatct gatcattatc aacttccaga ctcgcaccag acgctgaacc
    33301 gcttagtgcc agcaaaacac ccagcgacaa tctactgaat ttcgttgtca gtccagagga
    33361 tttacgcaaa ttaaccgtcg atgttttacc tactctgcga gttaattccg agcagacctg
    33421 aaatacctgt agggtgcaat tccatatcac gcgatagatt ctattcatat aaacgctcca
    33481 tatacaaaca atacagccac gaaagagatg agtcgtcaga cattaaaaat gaaacttatt
    33541 aaattgtcag aggtctgtat tgagtgttgg ttggaggtgg gaaggtgtca atttggctaa
    33601 tttgctaata ctttcgaatt gatagattaa aaattgcata gttcttattt tatttaaaaa
    33661 tgaaccatga gttattgttg cttaataaga aaaatgtttc ttttaataag aaaattacca
    33721 tcatcatatt taacaaagaa tagcattaat tgctaaaaaa gaactctatt aaaactcttt
    33781 ttcattcatt tatgccttta ggaatattcg ctataaaata agggttatgc ttaatatttt
    33841 aagaaaaata gcaatttccc cttgaatata ttactggcga tggaacgatg aatctgcaag
    33901 ctggcattta acaaaccagc tctaaaagaa gggaaataag aatactcaaa aactaacagc
    33961 cacggtcatc atgatgtggc tgtcaatgaa actataaccc aggcgctttc cataacgacg
    34021 tcgtcagccc gtgatcaacc agccccagct gatcctgata aactgcctgc cacttatcgc
    34081 gtgaatcctg ataaagttca tgtttttccg ggtctggtgt gtgcgtccgt tcccagcgaa
    34141 ccaggcgttc tccggtttct gccattgatg aaaaaattcc ggcaccgacg ccagctgcaa
    34201 tggcacatcc taatgcggtg gcttctttga ccaccggaat attgacaggt aatcccgaga
    34261 catcagcgag aatttgactc cataatttcc cttttgaacc tccgcctgca aagactaacg
    34321 atgaaggatg aatatccgag aaatcagcga tttgctgcaa gttacacgct gatacaatcg
    34381 ccgcattttc ttccagcgca cggaacaacg tcgctttgtt acatttatcc ggatcaatag
    34441 acaaattaat aaaggaaggc gcagcgtgat accaggtttt aaaccgcatt ctgtcggaga
    34501 atatcggcat tacgccccac gacccaggcg gcacccgact ggccatctct tccagcagcg
    34561 tataggtgtc gatgcctaaa cgttcggcaa tcagtttttc ttcggcacag aaagcatcgc
    34621 ggaaccagcg catggtgagt ccggtaaaaa agcttataga ttcagcttgt accatgccag
    34681 gaataacatg aggattaacg cgcacgttca tttctgggtc tgtcaccggc gcggctaagt
    34741 ttacaacttg ctgccagaat gtgccgccaa gaacagcggt ttgtgccgga cgcacaacgc
    34801 ctaaccccag gcaaccaagt tgcacgtcgc ctcctccaac gaccaccgga gtgcccgcct
    34861 tcagaccgca gagttccgcc gcttgtgaac ttaccacgcc cagcaatgtg ccggtttctt
    34921 tgacaggaga aagaatatcg gcccgcagtc cggccatatc cagcaatgca ggtttccagt
    34981 cacgggtggt tagatcaaga agtcccgtgg tgccagcgtt agagggatcc accgccagtt
    35041 cgccgctgag catataggcc agccagtcgc tgatcatggt gatggtcgac gcctgtcggt
    35101 agatatcaga acgatggtgc gccagccaaa gtaatctggg aatagcgctt aaagccagcg
    35161 tttgtccggt ggcgcgataa acttcgtttt caaaggtatt gttgtgcagt tctttaagtt
    35221 cgctaacttc gcgcgccgct ctggcatcca cattggcgca ggcccagatc ggtgttcctt
    35281 cattgttata taaaacaatg ccttcacgca tagaacatgc cgaaacggca gcgatatact
    35341 ccgggtctat gccggcgttg tgcagcgcct ggcgcataca ctcacacgcc agttgccagt
    35401 ttttgttgag atcaaattcc atagaaccag gaacgtccgg tactgccaga tgccgccact
    35461 ccgcctgtcc cactgctatt tgattgcctt ccaggttgaa tatcacagcc cgaatacttc
    35521 cggtgcctgc atccagcgcc atcaggtagt actttgattc tgaaggggta aagagtcgag
    35581 ccattagatt atcctcggct tatgcgttct gtctgttcct ctatacgttc tccatcattc
    35641 ccggtaataa ggtcatgcaa atttaactac gtaaaatcgc cgctgctgtg tcctgatcgg
    35701 taaccagtgc gttgatataa ccgcctttca ttgcagcggc aattgcttcg gctttatttt
    35761 ctccccctgc cacgccaacc cggacgggaa tagtcttcag cgcgcttaaa ggtaagccaa
    35821 tcagttcgtt atgtattttg atatccgtga caacgtcacc ttttgcatca aaaaagtagc
    35881 ctaaaatgtc gccaacagcc ccttttcgac caatcattaa ctgttcgccc tggctgatat
    35941 agccggagcg aatgattgtt gcgtcgtctt gttgactcac agccccaatg ccgacaatcg
    36001 ccacatccgc tgcttgtgcg gctaacagaa catctttgac gcaattttca ttttttagcg
    36061 tacgggcaat gtcagcggag gatgcccgca gcggagccgg aataatattc acactgcacg
    36121 ccgcattaag ctgcccgata cccgtcatat aagaaccgac gccaccggag agcgtgacca
    36181 ggcgaatttg ctgcgacgaa ataaaaccac ttaagcgttg cagcgtattc atggttgcct
    36241 cgccaaaacc aatcgccagc atctgttgtg gttgaagtaa actcatcaac atatgcgccg
    36301 cgcctatccc tagtcgcccg ccgacatcag catccgcaag cccagggatc acccggacat
    36361 gttgcagcga aaactgacga cgtaattgtg tttcatgttc cagacagcct tcaaagcgag
    36421 aattaatctg cacgcgaata atgccggact gatgcccttt ttccagtaat cgcgacactt
    36481 tcaagcgagt cagcccgaga cgatcgctga tctcgctctg ggtcagcccg tcgtggtagt
    36541 aaaaccacgc gatccgcgcg acctgttctt cttcacacat tccctgttct gaaattgccg
    36601 aatcgttgat tgtcataatt cattcttcac tttgaacata tttaaatctt taatgcaatt
    36661 gttcagttct tgcgcattta tatctgtgat ggcaaccaca gtttgactct acgagtatga
    36721 acaaacgcaa ccgtgaaaat caaaatagca taaattgtga tctattcgtc ggaaatatgt
    36781 gcaatgtcca cctaaggtta tgaacaaatt aaaagcagaa atacatttgt tcaaaactca
    36841 cctgcaaaac tgaacggggg aaatatgcaa acgagtgata cccgcgcgtt accgctactt
    36901 tgcgcccgct cggtttataa acagtattca ggggtcaatg tcctgaaagg catcgatttt
    36961 acgttgcatc agggggaggt ccacgccctg ctcggcggca atggtgccgg taaatcgacg
    37021 ttaatgaaga ttattgccgg tattacccct gctgatagcg gtacgctgga gattgggggc
    37081 aacaactacg ccagattaac gccagttcat gctcatcagc tgggtattta tctcgttccc
    37141 caggaaccgc tgcttttccc aagcctgtcg ataaaagaaa acatcctgtt tgggctggca
    37201 aaaaaacagc tctccatgca gaaaatgaag aacttgctgg cggcgctggg ctgccagttt
    37261 gatctgcata gtctggcagg atcgctggat gtcgccgatc gccaaatggt ggaaatcctc
    37321 cgcgggctga tgcgcgactc gcggattctg atcctcgatg aacctaccgc ctcgcttacc
    37381 cctgcggaaa ccgaacgctt gtttactcgc ttgcaagagc tgcttgctac tggcgtgggt
    37441 attgttttta tctcgcataa gctgccggaa attcgccaga ttgccgatcg gattagcgtg
    37501 atgcgcgacg gaactatcgc cttaagcggc aaaaccatcg aactgtctac cgacgacatt
    37561 attcaggcca ttacgccagc gctacgggaa aaatcgctct ctgccagcca aaaattatgg
    37621 ctggaattac ctggcaaccg cccacaacat accgcgggaa cgccggtgct gacactggaa
    37681 aatctgaccg gcgaaggttt caggaatgtc agcctgacgc ttaatgccgg agaaattctg
    37741 ggtctggctg ggctggtggg tgccggacgc acagaactgg ccgagacgct ctatggtctg
    37801 cgtactttgc gtggcggacg cattatgctg aatggtaaag agatcaatag attatccacc
    37861 ggagaacgtt tactgcgcgg tctggtttat ctgccggaag atcgccagtc atccggactt
    37921 aatctcgatg cttcactggc gtggaacgtc tgcgccctta ctcataacct tcgtggattc
    37981 tgggcgaaaa ccgcgaaaga taatgccacc ctggaacgtt atcgtcgggc gctgaatatt
    38041 aaatttaacc aaccggaaca agctgcacgg acattatccg gtggcaacca gcaaaaaatc
    38101 ctgattgcca aatgcctgga agcttcgccg caagtattga ttgtcgatga gccgacgcgc
    38161 ggcgtggatg tctcggcgcg taatgatatc taccaactgt tgcgcagcat cgccgcacag
    38221 aatgtggctg tgctgcttat ctcctctgat ctcgaagaga tcgaactgat ggcagatcgc
    38281 gtgtatgtga tgcatcaggg cgaaattgcc cactctgcac tgaccgggcg cgatattaat
    38341 gtcgagacca ttatgcgcgt tgccttcggc gatagtcagc gtcaggaggc gtcatgctga
    38401 aatttattca gaacaaccgt gaaatcacgg cactgctggc ggtggtgctg ctgtttgtat
    38461 tacccggttt tctcgaccgc cagtatttaa gtatgcaaac gctgaccatg gtttatagca
    38521 gcgcgcaaat cctgatccta ctggcaatgg gcgcgacgct ggtgatgctc acgcgcaata
    38581 tcgatgtttc ggtaggttcg attaccggaa tgtgcgcagt gctgttggga atgttactga
    38641 acgcggggta ttcactacct gttgcttgtg tcgcgacttt actgcttggt ttgctcgcgg
    38701 gatttttcaa cggtgtcctg gtcgcgtggc taaagatccc tgccattgtc gccacccttg
    38761 gcacgttagg gttgtacaga ggcatcatgt tgctgtggac tggcggtaaa tggattgagg
    38821 gattacccgc cgaactgaaa caactctccg ccccgctgct gtttggcgtt tccgcgattg
    38881 gttggttgac gataattctg atggcattta tggcctggct gctggcgaag acggcgtttg
    38941 gacgcagttt ttatgccacg ggcgataatt tacagggcgc tcgtcaactg ggcgttcgta
    39001 cggaagccat tcgcattgtg gcattttcgt tgaacggctg catggcggca ctggcgggaa
    39061 ttgtgtttgc ttcgcagatt ggttttatcc ccaaccagac cggtaccggg ctggagatga
    39121 aagcaattgc agcctgcgtg ctgggcggca ttagtttgct cggcggttcc ggcgcgatca
    39181 tcggtgcggt actcggcgca tggttcctga cgcagatcga tagtgtactg gtgctgttgc
    39241 gcattccggc atggtggaat gattttatcg cgggcatggt tctgctggcg gtgctggtgt
    39301 ttgatggacg cctgcgttgt gcgctggaac gtaatctgcg gcggcaaaaa tatgcccgct
    39361 ttatgatgcc accgccatcc gttaaacccg cttcgtcagg taaaaaacgg gaggccgcgt
    39421 aatgcgtatt cgctacggtt gggaactggc tcttgccgca ctgctcgtta ttgagattgt
    39481 tgcatttggt gcaattaacc cgcggatgtt agatctcaat atgttgctgt tcagcaccag
    39541 tgactttatt tgcattggca ttgtcgccct accgctgacg atggtgattg tcagtggcgg
    39601 gatcgatatt tcgtttggtt cgactatcgg cctctgcgcc attgcattgg gcgtactgtt
    39661 tcaaagtggt gtgccgatgc cgctggcgat tcttctgacc ttactgctcg gcgcattgtg
    39721 cgggttgatc aacgccggat taattatcta taccaaagtt aacccgctgg tgattacgct
    39781 tggcacgctg tatctgtttg ccggaagcgc cctgctgctt tccgggatgg ccggagcgac
    39841 ggggtacgaa ggtattggtg gattcccgat ggcgtttaca gatttcgcta acctggatgt
    39901 gctgggactc cctgttccgc tgattatctt cctgatatgt ctcctcgttt tctggctctg
    39961 gctgcataaa acccatgccg gacgtaacgt gtttttgatt gggcaaagcc cgcgcgtggc
    40021 gctttatagc gcgattccag ttaaccgtac cttatgtgcg ctctatgcca tgacggggct
    40081 ggcgtctgcg gtcgccgctg tgctgctggt gtcgtatttt ggttcagcac gttccgatct
    40141 cggtgcgtcg tttctgatgc ccgccatcac cgccgtggtg cttggcggtg ccaatattta
    40201 tggtggttcc ggttccatta tcggcaccgc cattgcggtt ttactagtgg gatatttgca
    40261 acaaggtttg caaatggcag gagtgccaaa tcaggtgtcc agcgcccttt ccggtgcgct
    40321 acttatcgtc gttgtcgtag gtcgttccgt tagcctgcat cgccagcaaa ttaaagagtg
    40381 gctggcgcgt cgggccaata acccattgcc ataaaggata tcttcatgac acttcatcgc
    40441 tttaagaaaa tcgccttact tagcgcgctt ggcattgccg caatctctat gaatgtgcag
    40501 gccgcagagc gtattgcatt tattcccaaa ctggttggcg tgggattttt taccagcggt
    40561 ggcaacggcg cacaacaagc gggtaaagag ctgggcgttg atgtgaccta cgacgggccg
    40621 acagaaccca gtgtttctgg tcaggtacag ttgattaata actttgtcaa tcaaggttat
    40681 aacgccatta tcgtttctgc ggtttcgcct gatggcttgt gtccggcact gaaacgcgcc
    40741 atgcaacgtg gtgtgagagt gctgacctgg gactctgata ctaaaccgga gtgccgctct
    40801 tactacatta atcagggaac gcccgcccag ttgggaggta tgttgatgga tatggcggcg
    40861 cgtcaggtga ataaagacaa agccaaagtc gcgtttttct actcaagccc caccgttacg
    40921 gaccaaaacc agtgggtgaa agaagcgaaa gctaaaatcg ccaaagagca tcctggctgg
    40981 gaaattgtca ctacgcagtt tggctataac gatgccacta aatcattaca aaccgcagaa
    41041 ggaatattaa aagcgtatag cgatctcgac gccattatcg cccccgacgc caacgccctg
    41101 cccgctgccg cacaagccgc agaaaacctg aaaaatgaca aagttgcgat tgtcggattc
    41161 agtacgccaa acgtgatgcg tccgtatgtg gaacgcggca cggtgaaaga atttggcctg
    41221 tgggatgtgg ttcagcaagg caaaaattca gtttatgtcg cggatgaatt attgaaaaaa
    41281 ggatcaatga aaacgggcga caagctggat attcagggcg taggtcaggt ggaagtctcg
    41341 ccaaacagcg ttcagggcta tgactacgaa gcggatggta atggcatcgt actgttaccg
    41401 gagcgcgtga tattcaacaa agagaatatc ggcaaatacg atttctgatg tgcattactt
    41461 aaccggagta cgttatggca gatttagacg atattaaaga tggtaaagat tttcgtaccg
    41521 atcaaccgca acaaaatatc ccttttaccc tgaaaggttg cggtgcactg gattggggaa
    41581 tgcagtcacg cttatcgcgg atatttaatc cgaaaacggg taaaaccgtg atgctggcct
    41641 ttgatcatgg ttattttcag gggccgacta ccggacttga acgcattgat ataaatatcg
    41701 ccccgctgtt tgaacatgcc gatgtattaa tgtgtacgcg cggcattttg cgcagcgtag
    41761 tcccccctgc gaccaataag ccggtggtac tgcgggcgtc aggtgcgaac tctattctgg
    41821 cggaattaag taatgaagcc gtggcgttat cgatggatga tgccgtgcgc ctgaacagtt
    41881 gcgcggtggc ggcgcaggtt tatatcggca gcgaatatga acatcagtcg atcaaaaata
    41941 ttattcagct ggtcgatgcc ggaatgaaag tgggaatgct gaccatggcc gtgactggcg
    42001 tgggcaaaga tatggtgcgc gatcagcgtt atttctcgct cgcgactcga atcgccgctg
    42061 aaatgggggc gcagattatc aaaacctatt atgtcgaaaa aggttttgaa cggattgttg
    42121 ccggatgtcc ggtacccatt gttattgctg gcggtaaaaa attaccggag cgcgaggcgc
    42181 tggaaatgtg ctggcaggct atcgatcagg gcgcttctgg tgtggatatg gggcgtaata
    42241 ttttccagtc tgaccatccg gtggcgatga tgaaagccgt acaggcggta gtacaccata
    42301 acgaaacggc tgatcgggca tatgaactct atctgagtga aaaacagtaa ctgcggatta
    42361 aggagaagaa acatgcacgt cacactggtt gaaattaacg ttcatgaaga caaggttgac
    42421 gagtttatcg aagtttttcg ccagaaccac ctgggctcgg tacaggaaga aggcaatttg
    42481 cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatatcta cgaagcttat
    42541 aaagatgaag acgcagtggc gttccataaa accacgcccc actacaaaac ctgtgtcgca
    42601 aaactggaag ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg gttgatgccg
    42661 tgaggcgaat ttatcaattt tatctacaat tggggtaacg cgctgacggg agtaaaaaaa
    42721 tgtctgactg gaacccctct ttatatctgc actttgccgc tgaacgatcg cgtccggcgg
    42781 tggagctgct tgccagagtg ccgctggaga atatcgaata cgttgccgat cttggctgtg
    42841 gcccaggtaa cagcaccgcc ctgctgcatc aacgttggcc tgcggccagg ataacaggca
    42901 tcgactcgtc tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg
    42961 tggaagcaga catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg
    43021 cctcactgca atggctgccc gaccactacg aattgttccc tcatctggtt tctttactta
    43081 attcgcatgg tgtgttggca gtacagatgc cagataactg gctagagcca acccatgtgc
    43141 tcatgcgcga agtcgcctgg gaacaaaatt atccggatcg cgggcgtgag ccgctggctg
    43201 gcgttcatgc ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa
    43261 ccacttacta tcaccagatg ccgtcgcacc aggcgattat cgattgggtg accgccactg
    43321 gattacgtcc gtggttacag gatctgaccg agagcgaaca gcagcatttt cttacgcgct
    43381 accatcagat gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat
    43441 ttccgcgtct gtttattgtt gcccggcgta cggagtaaat cttacgtcag ctggtaatga
    43501 cgatcgggaa gaatttttgc cggaatttct gcttcatcgt tcatctgtaa caggtcaatt
    43561 tcaatagcgt tgcagatggc atccagcggt aaatcattgt tttcagtacc gaagggatct
    43621 tccagttctt ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata
    43681 aaaggcgtca tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga
    43741 taaacggtac gatgcaaaat cagggtgtag gcaaagggaa ttggcgtata ggcaatgcgc
    43801 tcgcagcctg ccagaaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg
    43861 atatctgaaa gttgtccatt gcgtcgctga accgccaacc actcccccat tattaacaag
    43921 atgcggttag ctggagagtt cgaagccagt acacgctgaa gatcttccgt cttgagataa
    43981 tgagccagca cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag
    44041 gcgatttgca gccgggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc
    44101 tcgcgcagta acgaccgcga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg
    44161 taacgggcgt aaccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga
    44221 atgctgaacg gtgcgagggt gaatttaatg cccaaatgcg tgtaccaggg cagcatgaaa
    44281 ataacagcga tagaaaagag aaaattgagt agtaagcgcg aggatatctt ggataatact
    44341 gaaccgtgcc agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt
    44401 atcttcaggc gtggaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacac
    44461 tacttacaaa ttacctacaa gcttacaaca tttacaggtg tggcatatgg cacccgttaa
    44521 tgataaaggc acataggatg ttgtaactaa tatagtgaaa taagaaaccc ggtcgaaacc
    44581 gggttcagag tattagcaca acggacgtac agcttcgcgc attccttttt cgagaatcac
    44641 atccaggtca ttagccacct gctcgaccag accaggcact tgcgtcaggt cctgctccca
    44701 gtggtctttt tctgccaaaa caatcgctac cagttcctgg gtaccgataa cacggtcacg
    44761 atgctgactc cagagttgct gataacgttc cagccagtgt gcatcgtcct gtaccggata
    44821 tgtttctcca ttacgctcac cgcgataaaa cgcaatcaat gccgctaatg cgaaagtcag
    44881 gcgcgccgga agtgtgccgt ttgccttctg ccctgccagc agttgcggca ggatgcgggt
    44941 gcggaacttg gtcataccgt tgagcgcgat agacagcagc tgatgcttga tgtacgggtt
    45001 acggaaacgt ccggtgactg cactggcgaa agattccagt tcatcacgag gcaaatccag
    45061 taccggaata atttcttcgt agatggcttt ttcaacgaat gcgcaaattt cagcatcgtt
    45121 catcgcttca cctacggtat ccagccctgc ctggaacgcc accggcacca gcgcggtgtg
    45181 cgcaccgttg aggattgcca ctttgcgttc tttgtacggt ttaatatcgt caacaatcag
    45241 cacgttgagt ggatatttgt ccaggcacag ctcagtcgct aaggatttcg gcccctgaat
    45301 cacaaacagg taaaagtgtt cagcggtgtc gaggaaacca tcgtgataac ccagttcttc
    45361 ttccagttta gccacttcat cacgcggata gccggtagcg atacggtcca ccagcgtaga
    45421 acagaagctg ttagcctgat ccagccattg aataaatgct tctggtagtg cccactcttg
    45481 cgcatagcgc agcacaagtt cacgcaacgc gtcgccattg tagtcgatca actcacacgg
    45541 gatgatgatc caacctttat cctgcgctcc gttgaaatgg ctaaaacgtt cgaacagcag
    45601 acgggtcagt tttgccggat aacttaccgc tggcgcgtca tcgaatttat cgcccgcatg
    45661 gtagctgata cccgcttcgg tggtgttaga gaaaacaaag cgcatttccg ggttgtgcgc
    45721 cagtttcagg aattcatcgt attcactgta gacgctgatt tcacgattaa cagagcgaat
    45781 cagacgcgcg tcgctgaccg cttccccctt ctcgttcaga ccacggataa tggtggtata
    45841 cagaccatcc tgcgtgctca gtgacggcgg gaatgaagtt tcaatcggac gaacaacgac
    45901 cacgccagaa ttcagatcgg tgtgctcatt caggagatcg atttgccagt caacaaaggc
    45961 gcgcaggaag ttaccttcac caaactgaat gatacgttct ggatactgtg caccgggaaa
    46021 atcgcgacga tttagtgttt tcacaatggg ttcccttctg attagtcata caacctgttt
    46081 gaattggtac gacaggttag caaactttaa tacgccgaac ccctgttttg atcaactcct
    46141 gatgattaat gagcagtttt atgagaaaag tgtggcgcgg atcatggttt aatcgaggaa
    46201 ctacgccttt tattcgctca ataaagtggt agaacacatt ccgttaaaat cgctgtgaat
    46261 gaataaagat gtcagacaac ttcctcaccg taacgcatag tgctggtacg gttgcgccca
    46321 tctttcttcg agcgatacag gcatgtatcg gcttcgacca tcaatttatt aaagtcatcg
    46381 gtcagcgttc ggtacgatgc gcaaccacta ccgacgccaa tacttaccgt gagataaagc
    46441 gttttttgtt gccaggtgaa cggttgcagt tcaacgcctt tacggatttt ttccgccatt
    46501 agcagaccat cgacaggatt caccgacggc actgcaacag caaattcttc gccgccaatt
    46561 cgcgccacca gccccttatc accgacaatc ttctgaatat gctgggcaaa cacgcttaac
    46621 actttatcgc cacattcatg tccatagtta tcgttaatgc ttttgaaata gtcgatatca
    46681 agcagcatga cagtcagatg ttgtgtctgt ttcagcgatg gacttttcag cgcctcataa
    46741 agaccggacc gtgagtaaac ctgagtcaga aaatcaaagt cggctcgcag cgcaacttgc
    46801 ttcattagcg aattgatcgc tgccacgcta aaagaaacca taattgggca tatcgccatc
    46861 gtggcaatac cgagacgtgc ggagaacatt tgcggaatgg agaacggcga accgaccgag
    46921 atatcaatca ctgaatttgc caccagtacg atttcaaccg caccggtgac aaaggtcaac
    46981 agacatgtta cctgcggcgt atagcgcact gcacaccaga ttaatgcagg cagcgggaac
    47041 gccagactcc ccgctccgcc aatgactacc gaggcaataa ccgacacaat aagcgcgata
    47101 gcgggcatca tctgttctgc tttaaagcgc ggcagcactc caggaattgc caacgtcagc
    47161 atacaaggca cgatcaacac gcccgttgag aattgctcac tgaaccagtc ggcaagcaaa
    47221 ggccagaaat ccagactatc aatactgacc gaaccaatcg ccccgacaat agcgcataat
    47281 aatgcggcaa tcagacagta attaaatagc cgtaaggcac ttaccggctc atacttattt
    47341 ttccccagac gcttatcccg cgcgaccagt aaggcgacgg taacaataaa catcatattg
    47401 gagaaattga tagccagtga aaccagcccc cattcggtgg ttatggcatc ataaaccagc
    47461 attgccacat aactgatcgc atagtaatgc agtcggttaa gccagacata gcgggcaaac
    47521 ccccccgcca ttacgccatt cagcggccag aaaagagaaa gttcgtgaac cagccgtaac
    47581 tctgcaccaa tgaaatagaa cagcgtggtt agcgcaaaaa tagcgatgct attacgtagc
    47641 agatgacctt cctggaacaa acggaaagtt gaaatgggct gtacatgcat taattatctg
    47701 tccatacaat tgcgggtggc aattatctca acgtaataag taaattctta taaaggatag
    47761 catgttcgct gacagcggcg gcaaataaag atatcctggg aatgctattt tatcgttttc
    47821 cgcaattatc atggctgatt catctgtata accaaccagt gatagattac cgccacgata
    47881 taacgttgtt gttcctcgct taacgaaaca ccctgcggaa tgttatgcca cttagccaga
    47941 caaccgcgac aacaggttgc agtggcgtgc tgagcgataa acaccggatg cccgcgcatg
    48001 ggggtttgct taccgtcgtt agccggtaat gccggagcaa gacgtttagc gacaaaatcc
    48061 gccgcatgtt gttcgatgac tggcgcgcct ttctccagac aatactgacg ctctttcaca
    48121 cccaaaccaa agcgagagcg aaattttgaa cgggataaac gcgcgaagag tggatcgaga
    48181 gactgcatta ataaaccgaa cgccctaatt gtttcgtcaa ttgttcaagg acggcgatac
    48241 ccgcaagcga gttaccggca tcatccagtt ccgggctcca gactgcgatg gccatttcat
    48301 gcggaacaat cgccacaata ccgccaccaa cgccagattt cgccggtagt cccacccgcc
    48361 aggcaaactc ccccgcgttc tggtacatac cactggtcgc catcagtgcg ttaatttgcc
    48421 gcgcctgcat tggcgtcacc acaggttcat caatatgaat agctttcccc tgattagcca
    48481 gaaagacaaa cgtccgggcc agctctacac agctcatttt cagagcgcag taatgaaagt
    48541 agttttgcag aacggttgtc acgtcatgat ggaaattgcc aaacgacttc atcagccagg
    48601 cgatagccgc atttcgcgcg gagtgttcaa attcggaacg cgctaccacc gtatcgtagg
    48661 aaatatcaga cacaccgctt aagccgcgca cgacttccag catacgttgc cgaggagcgc
    48721 ttaatcgccc ttgcaacata tcgcagacca ccagcgcacc ggcattaata aacggattac
    48781 gcggaatacc ctgctccatt tccagttgca ctaaagaatt gaacggtgat ccagacggat
    48841 ctttgccgac gcgttgccag atttcctctt cggagtaatg acgcatggcg acaacgagac
    48901 tcagcacttt ggaaatagac tgaatggaaa aacgttcttg cgcgtctccg gcctgaaaaa
    48961 gctgtccgtc aacggtacag atagcaatgc ccaatcggga accgtctacc gtagccagcg
    49021 ccggaatata atccgcgact ttaccctgac caatgagcgg ccgcacttgc cgcaagatgt
    49081 tttctaaaat tgcattatcc atggcgactg ccactttcta ctcctggacc gcaggtctga
    49141 aaagacctgc gagtatatca gagctgaata tgtcgcgtca gatccggtct ttccacaccg
    49201 tctggatatt acagaattcg tgtaagccga aatgggaaag ctcacgacca aagccactct
    49261 ttttcacgcc accaaaggcc actcgcgcgt cgctggcaca ataaccattg ataaacaccc
    49321 caccgcattc cagacgtgcc gccatctgtc cggcctgtgt ttcgtcagtg gtaaaaatgg
    49381 tcgctgaaag gccgaactcg ctatcattag ccagttccag tgcatgttct gcatctttcg
    49441 caatcgtgat tgccgcaacg gggccaaaca tttcttcccg aaacgcggtc atttctgggg
    49501 taacattcgc cagaaccgtt ggcggatagt agttacctgc cccagccatc ttttccccgc
    49561 ccagtaacaa acgcgcaccc tgcgccaggg ttttctccac ctgatgatgc agctcatcac
    49621 gtaaatcaaa acgagccatt ggtccgagag tgttctcttc gtcacgggga tcgcccattt
    49681 tcaaggctgc cgcagctgcc acaaaacgtt cggtaaatgc cgaagcaatt ccctcttcga
    49741 tgataaagcg ttttgccgct gcacaaacct gtccggtatt ctgataacgt ccggctaccg
    49801 ccgctttaac cgccagttcc agatcggcat cgttaagcac aataaatgga tccgaaccgc
    49861 ccagttccag tacgcatttt ttcagtgccg ctccagcctg tgcgccaata gccgctcccg
    49921 cacgaacact tccggtcacc gtgacagcag caatgcgcga gtctttaatc atctgactga
    49981 cgccgtcgtt gtcggcattc agccagccat atacgccttg tgggataccc gcatctttaa
    50041 acacctgggc aatgagctgt gcacagccca tcacattcgg cgcatgttta agtaagtagc
    50101 cgttacctgc aagaatgatg ggaaccgcgc cacgcatcac ctgccataac ggaaaattcc
    50161 acggcataat cgccagaatt gtccccaacg gtcgatactc aataactgcc tgctgatttt
    50221 ccaccagcgt aggttccgcc ttcagcattg ccggaccatg ttctgcatac cagtcacaca
    50281 aattcgccga tttcgccact tcagcgcgcg cctggttgat tggtttgccc atttcacggg
    50341 tgatcatttg cgccatttct tcgctacgga cacgcagagc cttaccgata tcgcgcagtt
    50401 tttcagcacg ataatctata tttgtctcgc gccagtcgcg aaagcctgct gccgccagct
    50461 gaagtgcgtt ttcgatatca tcagcgccag cccacggcag cacagaaagt tgttcaccgg
    50521 tggcaggatt tatcgaaatt gcatgagttg ccggagtaat ggtcatcggg gtatctcctt
    50581 tatgagttat ggtaaaaaga tacgcagatt tactcttgct ttaaaatgaa taatattaag
    50641 ccacttattc acgaatcgag aatattatgg atctgaccca actggagatg ttcaacgcgg
    50701 ttgccgaagc tggcagcatt acccaggctg cagcaaaagt gcatcgcgtg ccgtcgaatc
    50761 tcactacccg tctacgccag ctggaaacag aactgggggt tgatctgttt attcgcgaga
    50821 atcagcgttt acgtctctcg cctgccgggc ataacttttt acgctatagc caacaaattc
    50881 tcgcgttagt ggatgaagcg cggagcgttg tcgctggcga tgagccgcaa ggtttatttt
    50941 ctcttggttc gctggaaagc accgctgcag tgcgcattcc ggccacgctg gcgcaattta
    51001 accgtcgtta tcccaaaatt cagttttcac tttccaccgg cccttccggc actatgctgg
    51061 aaggtgtgct ggagggaaaa ctgaatgcag cgtttattga tgggcccatt aaccatactg
    51121 ccattgacgg gataccggtg taccgcgagg agctgatgat cgtcacgcca caaggacatg
    51181 cgccagtaac ccgcgccagt caggttaatg gcagtaacat ttatgccttc cgcgccaatt
    51241 gttcgtatcg tcgccacttc gagagctggt ttcatgctga cggtgccgct ccgggaacta
    51301 ttcacgagat ggagtcgtat cacgggatgt tggcctgtgt ggtcgcagga gcaggcattg
    51361 cgcttattcc acgctctatg ctggaaagta tgccggggca tcaccaggtt gaggcgtggc
    51421 cgttagctga gcaatggcgt tggttaacaa catggctggt ctggcgtcgc ggtgcaaaaa
    51481 cgcgtccgct tgaggcattt attcaactac tggatgtgtc tgactcggca aaacagagtt
    51541 agcaatgagc tatttttgat agttcttgcg gctaatatgc tctatatagt gatgctccga
    51601 tgacttatga ctatatgggg caaatatggt tacgccagta agcatcagca attacatatc
    51661 tcttcctgat gattttcccg ttcgtaatat tgcacctcag gtaaaagaag ttttaaagga
    51721 ttttattgat gcgctaagta caataatatg tgatgaagag tggcgtacgt ctttaaatat
    51781 cagctcagcc acaaaaaaga tatttaataa gcttgataac ttatcatata ttcagagaac
    51841 ctctttccgg ggtaacgaca cgctatacaa tgaaaaagtt caatttaaac tcacttatcc
    51901 tgacaggaat ggaagacaca aagaaaatat tgaatttcag gtagtgataa atttaagccc
    51961 tatttattta gataatttcc gccatgatgg ggaaattaat attttttgcg ctcccaaccc
    52021 aaagcctgtc actatggggc gcgtatttca gaccggcgtc gagcgtgtgc tctttctgtt
    52081 tctgaatgat tttattgaac aatttccaat gatcaaccct ggtgttccta tcaaaagagc
    52141 gcatacgcca catattgaac ccctgcatcc ggatcaccat accgctgcag attacttacg
    52201 ccagtttgat ttgcttgtcc tgaattttat ctctcgcggt aattttgtca tactcccccg
    52261 attatggaat aactctgagg ttcacagatg gttcgggaat aaagaaccta acttgctcac
    52321 cgctattctc gacataacgg acagcgaatt aaaagaggat ttattgcaaa gtctgatgga
    52381 ttcactaggt tctaacaaac atgtactacc cgaagtctgc atctgctttt tatccctttt
    52441 agcagaacaa gattttccac attttcagga cttgttttta tttttcgcca atatgttatt
    52501 gcactatcac caatttatga atcccaatga aagtgatttg attgacgtgt tgatgccagc
    52561 atcattaagt gatgataaaa ttatcaaaca tatggcgcgc aagaccctca aactttttgt
    52621 aaaaaatgaa acgccgccaa aagttactca tgaagacctg gtgaaaaaca ggcctcgctc
    52681 ccctgtcaga ccacctatac ccgcaacctc caaaacgcca gacctccctg aacgtcatta
    52741 aaccgtgatg tgaccgactc tctgacgcgt gaacgaatca gcgtcagaga aacggaaaac
    52801 gcgatccaga tcacaaatgc attgtattca catcattaac cgttttaaga tcatttcatc
    52861 actttttcgc aactcacccg ataatctgtt atgacaacaa acactgtttc ccgcaaagtg
    52921 gcgtggctac gggtcgttac gctggcagtc gccgccttca tcttcaacac caccgaattt
    52981 gtcccagttg gcctgctctc tgacattgcg caaagttttc acatgcaaac cgctcaggtc
    53041 ggcattatgt tgaccattta cgcatgggta gtagcgctaa tgtcattgcc ttttatgtta
    53101 atgaccagcc aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt gtttattgcc
    53161 agccacgtac tgtcgttttt atcgtggagt tttaccgttc tggtgatcag tcgcattggt
    53221 gtggcttttg cacatgcgat tttctggtcg attacggcgt ctctggcgat ccgtatggct
    53281 ccggccggga agcgagcaca ggcattgagt ttaattgcca ccggtactgc actggcgatg
    53341 gtattaggtt tacctctcgg gcgcattgtg gggcagtatt ttggctggcg aatgaccttc
    53401 ttcgcgattg gtatcggggc gtttattacc cttttgtgcc tgattaagtt acttcccttg
    53461 ctgcccagtg agcattccgg ttcattgaaa agcctcccgc tattgttccg ccgcccggca
    53521 ttaatgagca tttatttgtt aactgtggtg gttgtcaccg cccattacac ggcatacagc
    53581 tatatcgagc cttttgtgca aaacattgcg ggattcagcg ccaactttgc cacggcatta
    53641 ctgttattac tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa actgggtaat
    53701 cagtatgcgt ctgcgttggt gagtacggcg attgcgttgt tgctggtgtg cctcgcactg
    53761 ctgctacctg cggcgaacag tgaaatacac ctcggggtgc taagtatttt ctgggggatc
    53821 gcgatgatga tcatcgggct tggtatgcag gttaaagtgc tggcgctggc accagatgcc
    53881 accgacgtcg cgatggcgct attctccggg atatttaata ttggaatcgg ggcgggtgcg
    53941 ttggtaggta atcaggtgag tctgcactgg tcaatgtcga tgattggtta tgtgggcgcg
    54001 gtgcctgctt ttgccgcgtt aatatggtca atcattatat ttcgccgctg gccagtgaca
    54061 ctcgaagaac agacgcaata gttgaaaggc ccattcgggc cttttttaat ggtacgtttt
    54121 aatgatttcc aggatgccgt taataataaa ctgcacaccc atacatacca gcaggaatcc
    54181 catcagacgg gagatcgctt caatgccccc cttacccacc agccgcataa ttgcgccaga
    54241 gctgcgtaga cttccccaca aaataaccgc caccaggaaa aagatcagcg gcggcgcaac
    54301 catcagtacc caatcagcga aggttgaact ctgacgcact gtggacgccg agctaataat
    54361 cattgcaatg gttcccggac cggcggtact tggcattgcc agtggtacaa aggcaatatt
    54421 ggcactgggt tcatcttcca gctcttccga cttgcttttc gcctccggtg aatcaatcgc
    54481 tttctgttgc ggaaaaagca tccgaaaacc gataaacgcg acgattaagc cgcctgcaat
    54541 tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc agttgcccgg cgtaatacgc
    54601 caccatcatg atggcaaaga cgtacaccga ggccatcaac gactgacgat tacgttcggc
    54661 actgttcatg ttgcctgcca ggccaagaaa taacgcgaca gttgttaatg ggttagctaa
    54721 cggtagtaac accaccagcc ccaggccaat tgctttaaat aaatctaaca ttggtggttg
    54781 ttatcctgtg tatctgggtt atcagcgaaa agtataaggg gtaaacaacg ataaagtgtc
    54841 actctttagc tagccttgca tcgcattgac caaaacttga accgatttag caaaacgtgg
    54901 catcggtcaa ttcattcatt tgacttatac ttgcctgggc aatattatcc cctgcaacta
    54961 attacttgcc agggcaacta atgtgaaaag taccagcgat ctgttcaatg aaattattcc
    55021 attgggtcgc ttaatccata tggttaatca gaagaaagat cgcctgctta acgagtatct
    55081 gtctccgctg gatattaccg cggcacagtt taaggtgctc tgctctatcc gctgcgcggc
    55141 gtgtattact ccggttgaac tgaaaagagt gttatcggtc gacctggggg cgctgacccg
    55201 tatgctggat cgcctggtct gtaaaggctg ggtggaaagg ttgccgaacc cgaatgacaa
    55261 gcgcggcgta ctggtaaaac ttaccaccag cggcgcggca atatgtgaac aatgccatca
    55321 attagttggc caggacctgc atcaagaatt aacaaaaaac ctgacggcgg acgaagtggc
    55381 aacacttgag catttgctta agaaagtcct gccgtaaaca aaaaaagagg tatgacgatg
    55441 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
    55501 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc taaatggcac
    55561 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
    55621 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
    55681 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
    55741 gttccgccac ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
    55801 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
    55861 acttttatcc gcaatagcag ctgcgtttat tctcttttcc acgcagggca ttgcggaaca
    55921 aaccacgcag ccggttgtta cttcctgtgc caatgtcgtg gttgttcccc catcgcagga
    55981 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    56041 cgtgccctat tataatcaac acgctatgta gttattgtca ggctctgatc acagagcctg
    56101 atatctccgt aatatcttcc tgaagtctca aatattttcg ccattgttgg cgatgagatc
    56161 ccgataccag taaaacgacg ctttacgtat ccgttccagc gttcccttcc cttcgttgtc
    56221 tttatcgacg taaatcattc cgtagcgctt tttcatttcc cctgttccgg cagaaaccag
    56281 gtcaatgcag ccccacggtg tgtagccaat taagtcaaca ccatcttcaa caacggcttt
    56341 tttcatttcc cgaatatggg aagcaaagta atcaatgcga tagtgatcgt tcaccgtgcc
    56401 gtcagcttgt ctctggtcaa ccgcaccaaa tccattttcg acaataaaca gcggcaactg
    56461 gaaatgatcc cagaaccagt tgagtgaata acgtagccct gccggatcaa tttgccatcc
    56521 ccattcggat gtatcgatat aagggttgct gaccaaatct cgtggctcaa cataatccag
    56581 ttgcgggtta tctggcgaaa attgtgtcgt aaaagacatg tagtagctaa agccgataaa
    56641 gtcgacacaa cctctggcaa gaatcgcatt atcttctggt gtgatatcga ggttgaatcc
    56701 tttcctggca aagtaattca gcatatgttg cggataatat ccacgcgcat gaacatcagt
    56761 aaaccagtaa cgacgatgca tcgctttcgt ggccatcatc atatcgttgg gtgcacacgt
    56821 cagaggatag atggggcaca tggcgatcat acagccgata ttaaattcag gattgatcga
    56881 ttttccagtc tgtaccgcca gggcactggc aactaactcg taatgtaccg cctggtacat
    56941 tatttgttcg cgctcattga tatcttcctc tggcgaatac aagataccgg aattagtaaa
    57001 tggacacagg ctttcgctga aattcacctg attattgatt tcgttaaacg tcatccagta
    57061 ctttactttt tctttatagc gcgtgaagac cgttgatgcg aagcggataa aaaagtcgat
    57121 cagtttacgg tttcgccagc caccatattt tgtcaccaga tggtaaggca tctcaaaatg
    57181 cgaaagcgtc accacaggtt ccattccctg cttcaggcat tcatcgaaca gatcatcata
    57241 aaattttaaa ccctcttcat tcggctcctg ttcgtcacct tgcggaaaga ttcgtgtcca
    57301 ggcaatggaa gttcgaaagc atttgaatcc catctcggca aataactgaa tatctgtttt
    57361 atagcgatga taaaaatcaa ttgcttcatg attgggataa ttaagtccgt cgataacgcc
    57421 ttctgtcact tcacgcggca caccgtgagc gccagcagtc atcacatcag cgatactgat
    57481 gccttttcct ccttcattcc agccaccttc caactgatgc gcggctaccg cgccgcccca
    57541 taaaaaacct tttttaaatc ctgacataca caactcctta actaaaatag attaccacca
    57601 gacttccgcc tggatgccgg ccataaactc gtctttgctg ttctcattaa acttaaaatt
    57661 agataattcg ttatccagaa tattgatata cgttccatag aagcgaattt ctggtcgtga
    57721 acctaaaatg ctttcaccca ctttcaatgc atgccagagt gtcgttttat aagccgattc
    57781 atttagtgtt acaccctgct gtgttttgtt ctgttgctta aaccagccta attcaagccc
    57841 cgtctgattc catgtattcc agatccaggc cggtcttact acggtgcgaa tactgtcaaa
    57901 atcactatga gcgccacttt cataactata aacatcttcg ccatgagaat agacaagcgc
    57961 gttagccata ataatattgt cagtaagata catctcgccc tgagagatta aacgccaggc
    58021 gatcccattg gtatggtcac catagtaata gcgaccatgc gccatcgtgt tactggcatc
    58081 tgagaaactg gcaaaactgc tggcatagga attattggca acctgtaacg taaattcatt
    58141 aaacgtatcg cgttgcaggt tttgccgtaa aacagaagtc agcatccagc tatctttcat
    58201 ttcaaaataa ctgtcgtcat tttcattatc ttgttgttga tccgttttat ttggtgcgga
    58261 atatttagcc attaatgata atgttgcatc atcccataac gggatattgc gataacgaac
    58321 atccacagaa ttggtattca tctgactggt acgcgtaaaa tcacgagagt aaacatcgac
    58381 atcatcacgg cttaaggaca tatcaaacag ccctacacca agtgcccagt tttcaatccc
    58441 cacacccgca gcgacatccg tggttaaggt tttccagtcc agcatttgga tctcatattg
    58501 cgggagttta tgtttgccta cccagaagtc tgcctctggc gcgaagggta aaaaacctcg
    58561 cgttgtcaga taaatatcac tgaactgcat gatattttca ttggcactgt caccaaacca
    58621 ggcatcgtta tactgctcac ctacgttgcc atcataggta acgaccgcat ttgccgtttt
    58681 accgtcctga ttataaacac gctgatttaa ggttaagtca aaccaaccgc tcatctcgtt
    58741 accaaaacgt cccagagaac ccgctgcata agtttgtggt gagccatgat ttccggtacc
    58801 ccagcctgag cggaagtacc cctgatagct gaacccaata tcatccttta tatatttact
    58861 gatatctttc agggtcacgc tttcgatagt ggttttgtcg cctttacttt caataacggc
    58921 aacttgctgc gatccattta tagtatgcgc tgcggcagac gagttctgtt caccgttttc
    58981 atttttgatg ttatcagcga cgggagatgt cgctgatatt tcagcaagtg aggctgattt
    59041 gtatttattt tccgtaatac ttttttgtaa ggtcgaaagt cgggatttat atacccccag
    59101 ttcattctgt gttgctctca gctcttgttt attttgcgac aattcatttt caagcagttc
    59161 aaggcgctgc tcaatggtta attttgttgc caacgcgttc aacggaaaca atgctgtaat
    59221 aatagaaaaa gacaaaaggc tgacgttaag cgtctttata ttcatgactt atccttaaga
    59281 gtgtaataat ttcctccagc tatcgcactg gaggaaaaaa tcaggcatgg agctcttttc
    59341 ttaatgaaat aatttccctg gctaaatcct ggcataatat tgcattcatc aaatgatcct
    59401 gtgcatgaac cagaattaaa ttaactacta ttttgccaca accttcatcc tgactaatta
    59461 acgcggtctg aattttatgt gcctgaagac aggctaattc cgaactggcc attagctgag
    59521 ttgcgccctg ccagtctttt tgcccggcac aacgtatggc ttccatcgca tcagaacgtg
    59581 cctgtcccgc gttgatcagt aactccataa caagttcttc atctgcaaac atcaacatct
    59641 ccttcctgtg taaatgggat tatccgttag ttgtcatgtt attgaccttg ttggcggcaa
    59701 taacaaaagg taaatagatc attgtcgcca cacctaaaca taaaaaaccc accagtaacg
    59761 ccagccagtt tcccccggtt ccgaggaaag caattaaagg tccgggcgta gtccagggca
    59821 cggcataagc aaccggtgga ataatttcca tcgaaacaaa gaagtaacca atggcacagt
    59881 taacaatggg aaccagaata aacggcacca tcatgatggg gttaagtaca ataggaaggc
    59941 cgaacattat cggttcattg atattgaaaa taccggggat aaacgacatt tttgccagat
    60001 cacggtattc cgcacgctta gacgcgataa aaatagccaa caataacccc aacgtcatac
    60061 ctgaaccgcc gcagttggcg aatgcatcat taatagaagt ccaggtaatc ggatatggcg
    60121 cgccccaggt agtgccgtgt tgagcggccc aggagagatt ctccagatta gcctcagaaa
    60181 aaacagtttc tcgaattgcc gaggtggtat taggtccgtg gatccccagc acccacagga
    60241 aattacccac agcccccagg ataattaccg caaagatatt cgttcccata tgtttgagcg
    60301 gcgtctggat taatttgtaa atgagatcgt ttaatcctgc cggggatatc agtgtcaggc
    60361 aataattaag tgcggaaaag aacaccatga caaaaaatat tggcaataaa actttaaatg
    60421 aacgcgatac cgcaggaggt acagctgccg gcatcgttat ggtgattttt gggtttcgag
    60481 ctaagcgaca aaatatttca ctggtgatta atgcaacgat aacagcaaca aacaacccct
    60541 gtgcgccgag atatttggtc gtcaggaatg cttgcccatc tatcatctga tatggcgtat
    60601 aaacaataaa aaatgctcct attgccgtta atccgcataa aagatcatct tgctcatagc
    60661 taatcgccat atttctggcg accaggaaag caatcataat tgacatgata tttacagaac
    60721 cattcattac cggagtaaaa atagcttgtg ctttttcaag gttggggaaa atgctattca
    60781 gatgcagtaa tccggcaata aaaccgtctg gcgataatat ggcaaagtta attaatataa
    60841 ttaatgaact tgccatgata attggaaacg taaaaacgaa tccatctctc accgctgcaa
    60901 catgtttttg tgagtttaac tttatagcaa ctggaacaag aaaacgttcc attccacgtt
    60961 cgaatgaggt cattaatccc atttttttat acctgttgtt ctggatattt atacatccca
    61021 ataaagcatg tatgccatat ccattttatg atgtgtgaaa agttagttca ggtaatagtt
    61081 taataatatt agattcttac tcttgcagta gtttcatcgc aaattcaaga actgcctgtc
    61141 cgttcatggt gccatagtct ttcatctcga taacagctac aggcattttc ccctgtgcaa
    61201 cagcttcaat ctctggtttc tgaaagcgaa cttgcgggcc aagtaaaaca acgtcaactt
    61261 cgttattttt aattttccct ttagcctctg caatcgccaa tgcggaaata ttaacttcaa
    61321 gtgaaatagc gttagcatga tcaatcatac gtttaaccag catactggtt gacatgcccg
    61381 cagcgcaaac taacaatatt tttttcatat tttcccttac tggtatataa cagattacat
    61441 tgtgtaaata tcatatcccg cagtagctat acatcctggt acactgatca cagtaataca
    61501 gtgataaagt tgttcaacat cagggtgata attaaacgac tcggccatac gataaaccaa
    61561 tcaggactca actccttgtt ttcaatggct cttaaaaggt gcaaaagtga tttttcaaaa
    61621 gattgcccgc ttacttaaat ccgagatcaa tggctattca tggcatgttg gtgatttgtt
    61681 gccgtcagaa gctgaactcg ctgttcgtta taatgtttcg cgtaataccc tgcgtaaggc
    61741 attatccttg ctggaaggcg aaggaattat tcatagaaag catggttcag gaacatatat
    61801 tcaaaaaaag aattttgttg cacacattga tcacatgaac agcttcaatg aaattgcgca
    61861 taaaagcggc aaggaggcag gaagtcagat tatgaaattt gaagtgcagg atgcttctcc
    61921 ccctattgcg actgagctga atttagtgac tggtgagcag gtttattaca taaagagact
    61981 gcgattcatt gaggataatg cagcacaatt agaagaaacg tggatgtcag tggcacgttt
    62041 tcctgattta accgtagcac atatgcaaaa atccaaattt tcgtatattg agaacgaatg
    62101 cgggatcaaa atcattggca cctttgaaac tttctcccct acttttccta ccccagaaat
    62161 cgccagtatt ttacggatca gcccacggga tcccatactt aaaattcaga cccaggctgt
    62221 ggacagtaac tctattccgc tggattattc gttactttac agcaatattt tcgagttcca
    62281 ggtaaagtac tttttcccgc gataaaagca tacgcacaga gtgctggcat cacattctgt
    62341 gcgtatggtt tagcctctaa cctttaccgc tttacgccac cgcaagccaa atacattgat
    62401 atacagcccg gtcataatga gcaccgcacc gaaaaattgt agacccgtta agcgttcatc
    62461 caacaatagt gccgcacttg ccagtcctac tacgggcacc agtaacgata acggtgcaac
    62521 ccgccaggtt tcatagcgtc ccagtaacgt cccccagatc ccataaccaa caattgtcgc
    62581 cacaaacgcc agatacatca gagacaagat ggtggtcata tcgatagtga ccagactgtg
    62641 aatcatggtt gcggaaccat cgagaatcag cgaggcaaca aagaagggaa tgattgggat
    62701 taatgcgctc cagattacca gcgacatcac cgccgggcgc gttgagtgcg acatgatctt
    62761 tttattgaag atgttgccac acgcccaact aaatgctgcc gccagggtca acataaagcc
    62821 gagcatcgcc acatgctgac cgttcagact atcttcgatt aacaccagta cgccaaaaat
    62881 cgctaaggca atccccgcca attgtttgcc atgcagccgc tcaccgaaag taaatgcgcc
    62941 aagtacgata gtaaaaaacg cctgtgcctg taacaccagc gaagccagtc cagcaggcat
    63001 accgaagtta atggcacaaa aaagaaaagc aaactgcgca aaactgatgg ttaatccata
    63061 ccccagcaac aaattcagcg gtactttcgg tcgtgcgaca aaaaagatgg ccggaaaagc
    63121 gaccagcata aagcgcaaac cggccagcat cagcggtggc atgttatgaa gccccacttt
    63181 gatgaccaca aaattcagcc cccatacgac cactaccagt agcgccaaca ccccatcttt
    63241 tcgcgacatt ctaccgcctc tgaatttcat cttttgtaag caatcaactt agctgaattt
    63301 acttttcttt aacagttgat tcgttagtcg ccgattacga cggcattaat gcgcaaataa
    63361 gtcgctatac ttcggatttt tgccatgcta tttctttaca tctctaaaac aaaacataac
    63421 gaaacgcact gccggacaga caaatgaact tatccctccg acgctctacc agcgcccttc
    63481 ttgcctcgtc gttgttatta accatcggac gcggcgctac gctgccgttt atgaccattt
    63541 acttgagtcg ccagtacaac ctgagtgtcg atctaatcgg ttatgcgatg acaattgcgc
    63601 tcactattgg cgtcgttttt agcctcggtt ttggtatcct ggcggataag ttcgacaaga
    63661 aacgctatat gttactggca attaccgcct tcgccagcgg ttttattgcc attcctttag
    63721 tgaataacgt gacgctggtg gtgctctttt ttgccctcat taactgcgcc tattccgttt
    63781 ttgctaccgt gttgaaagcc tggtttgccg acaatctttc gtccaccagc aaaacgaaaa
    63841 tcttctcaat caactacacc atgctcaaca ttggctggac catcggtccg ccgctcggca
    63901 cgctgttggt gatgcagagc atcaatctgc ccttttggct ggcagctatc tgttccgcgt
    63961 ttcccatgct tttcattcaa atttgggtaa agcgcagcga gaaaatcatc gccacggaaa
    64021 caggcagtgc ctggtcgccg aaagttttat tacaagataa agcactgttg tggtttacct
    64081 gctctggttt tctggcttcc tttgttagcg gcgcatttgc ctcgtgcatt tcacaatatg
    64141 tgatggtgat tgctgatggg gattttgccg aaaaggtggt tgcggttgtt cttccggtta
    64201 atgctgccat ggtagttacg ttgcaatatt ccgtgggccg ccgccttaac ccggctaaca
    64261 tccgcgcgct gatgacagca ggcaccctct gtttcgtcat cggtctggtt ggttttattt
    64321 tttccggcaa cagcctgcta ttgtggggta tgtcagctgc ggtatttact gtcggtgaaa
    64381 tcatttatgc gccgggcgag tatatgttga ttgaccatat tgcgccgcca ggaatgaaag
    64441 ccagctattt ttccgctcag tctttaggct ggcttggtgc cgcgattaac ccattagtga
    64501 gtggcgtagt gctaaccaac ctgccgcctt cctcgctgtt tatcatctta acgttggtga
    64561 ttattgctgc gtgggtgctg atgttaaaag gaattcgtgc aagaccgtgg gggcagcccg
    64621 cgctttgttg atttaagtcg aacacaataa aaatttaatt cagccttcgt ttaggttatc
    64681 tctgctaata tctttctcat tgagatgaaa attaaggtaa gcgaggaaac acaccacacc
    64741 ataaacggag gcaaataatg ctgggtaata tgaatgtttt tatggccgta ctgggaataa
    64801 ttttattttc tggttttctg gccgcgtatt tcagccacaa atgggatgac taatgaacgg
    64861 agataatccc tcacctaacc ggccccttgt tacagttgtg tacaaggggc ctgattttta
    64921 tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg
    64981 ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg acacagtagc
    65041 atcagttttc tcaatgaatg ttaaacggag cttaaactcg gttaatcacg ttttgttcgt
    65101 caataaacat acagcgattt cttccggttt gcttaccctc atacattgcc cggtccgctc
    65161 ttccaatgac cacatccaga ggctcttcag gaaatgcgcg actcacacct gctgtcacgg
    65221 taatgttgat atgcccttca gaatgtgtga tggcatggtt atcgactaac tggcaaattc
    65281 tgatacctgc acgacatgct tcttcatcat tagccgcttt gacaataatg ataaattctt
    65341 cgccaccgta gcgataaacc gtttcgtaat cgcgcgtcca actggctaag taagttgcca
    65401 gggtgcgtaa tactacatcg ccgattaaat gcccgtaggt atcattaacc aatttaaatc
    65461 ggtcaatatc caacaacatt aaataaagat tcagaggctc agcgttgcgt aactgatgat
    65521 caaaggattc atcaagaacc cgacgacccg gcaatcccgt caaaacatcc atattgctac
    65581 ggatcgtcag caaataaatt ttgtaatcgg ttaatgccgc agtaaaagaa agcaaccctt
    65641 cctgaaaggc gtcgaaatgc gcgtcctgcc agtgattttc aacaatagcc aacattaatt
    65701 ctcgaccaca gttatgcata tgttgatggg cagaatccat tagccgaacg taaggtaatt
    65761 catcgttatc gagtggcctc agatgatcaa tccaccgacc aaactggcac agtccataag
    65821 aatggttatc cgttatttct ggcttactgg catctctcgc gaccacgctg tgaaacatac
    65881 tcaccagcca ctggtagtgg gcatcgatag ccttattgag atttaacaag atggcatcaa
    65941 tttccgttgt cttcttgatc attgccactc ctttttcaca gttccttgtg cgcgctattc
    66001 taacgagaga aaagcaaaat tacgtcaata ttttcataga aatcctaagt tatgagtcat
    66061 ctctgagata acattgtgat ttgaaacaaa atcagcagat aaaaaagtgt ttaattctgt
    66121 aaattaagcc tgcattatcg caattaaaag aatgacaaat agcata
//
LOCUS       DAPOZO010000014        65790 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPOZO010000014 DAPOZO010000000
VERSION     DAPOZO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.014"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1385)
                     /gene="asnS"
                     /locus_tag="ROI21_000950"
     CDS             complement(<1..1385)
                     /gene="asnS"
                     /locus_tag="ROI21_000950"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDX5109905.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPR"
     gene            complement(1554..2756)
                     /gene="pncB"
                     /locus_tag="ROI21_000951"
     CDS             complement(1554..2756)
                     /gene="pncB"
                     /locus_tag="ROI21_000951"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDX5109906.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3022..5634
                     /gene="pepN"
                     /locus_tag="ROI21_000952"
     CDS             3022..5634
                     /gene="pepN"
                     /locus_tag="ROI21_000952"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDX5109907.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLDGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAT
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(5676..6443)
                     /gene="ssuB"
                     /locus_tag="ROI21_000953"
     CDS             complement(5676..6443)
                     /gene="ssuB"
                     /locus_tag="ROI21_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDX5109908.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(6440..7231)
                     /gene="ssuC"
                     /locus_tag="ROI21_000954"
     CDS             complement(6440..7231)
                     /gene="ssuC"
                     /locus_tag="ROI21_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDX5109909.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7243..8388)
                     /gene="ssuD"
                     /locus_tag="ROI21_000955"
     CDS             complement(7243..8388)
                     /gene="ssuD"
                     /locus_tag="ROI21_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDX5109910.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRQVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(8385..9344)
                     /gene="ssuA"
                     /locus_tag="ROI21_000956"
     CDS             complement(8385..9344)
                     /gene="ssuA"
                     /locus_tag="ROI21_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDX5109911.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKSNISSVLAK
                     NYQMLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9337..9912)
                     /gene="ssuE"
                     /locus_tag="ROI21_000957"
     CDS             complement(9337..9912)
                     /gene="ssuE"
                     /locus_tag="ROI21_000957"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDX5109912.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALNTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10268..10807
                     /locus_tag="ROI21_000958"
     CDS             10268..10807
                     /locus_tag="ROI21_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5109913.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAITPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            10890..11591
                     /locus_tag="ROI21_000959"
     CDS             10890..11591
                     /locus_tag="ROI21_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDX5109914.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKSLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFPLPKGAQNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            11616..14216
                     /locus_tag="ROI21_000960"
     CDS             11616..14216
                     /locus_tag="ROI21_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDX5109915.1"
                     /translation="MPRTHRQHSLLTSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFERGQKITPGVYRVDIVLNQTMVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACAPLAEIIPDASVTFDVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSSDSDSTDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSINSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNDQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMLGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNHLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGDYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHTTQNLG
                     LTETLLDDGNLSYSVQQGYNSEGETANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSRGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIVLDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGGRVLMKTSTQGIPLRF
                     GAIATLDGVQTNSGIIDDDGSLYMAGLPAKGTITVRWGEAPDQICHISYQLTEQQINA
                     AITRMDAICR"
     gene            14207..15277
                     /locus_tag="ROI21_000961"
     CDS             14207..15277
                     /locus_tag="ROI21_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5109916.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAVPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            15289..15831
                     /locus_tag="ROI21_000962"
     CDS             15289..15831
                     /locus_tag="ROI21_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5109917.1"
                     /translation="MKKKTIFQYVILFFSFLNTHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            15839..16354
                     /locus_tag="ROI21_000963"
     CDS             15839..16354
                     /locus_tag="ROI21_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX5109918.1"
                     /translation="MLKRIIWILCLLGLTGSCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGMQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            16320..17058
                     /locus_tag="ROI21_000964"
                     /pseudo
     CDS             16320..17058
                     /locus_tag="ROI21_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            17169..18179
                     /gene="pyrD"
                     /locus_tag="ROI21_000965"
     CDS             17169..18179
                     /gene="pyrD"
                     /locus_tag="ROI21_000965"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDX5109919.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            18353..18895
                     /gene="zapC"
                     /locus_tag="ROI21_000966"
     CDS             18353..18895
                     /gene="zapC"
                     /locus_tag="ROI21_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDX5109920.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFERKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(18892..20001)
                     /gene="ycbX"
                     /locus_tag="ROI21_000967"
     CDS             complement(18892..20001)
                     /gene="ycbX"
                     /locus_tag="ROI21_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDX5109921.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRIGDEVEILATAPAKIYGAAA
                     ADDTVNITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            20245..22353
                     /gene="rlmKL"
                     /locus_tag="ROI21_000968"
     CDS             20245..22353
                     /gene="rlmKL"
                     /locus_tag="ROI21_000968"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDX5109922.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYNSHLYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            22365..24272
                     /locus_tag="ROI21_000969"
     CDS             22365..24272
                     /locus_tag="ROI21_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5109923.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            24402..25655
                     /gene="pqiA"
                     /locus_tag="ROI21_000970"
     CDS             24402..25655
                     /gene="pqiA"
                     /locus_tag="ROI21_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDX5109924.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            25660..27300
                     /gene="pqiB"
                     /locus_tag="ROI21_000971"
     CDS             25660..27300
                     /gene="pqiB"
                     /locus_tag="ROI21_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDX5109925.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPMDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            27297..27860
                     /gene="pqiC"
                     /locus_tag="ROI21_000972"
     CDS             27297..27860
                     /gene="pqiC"
                     /locus_tag="ROI21_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDX5109926.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            28116..28283
                     /gene="rmf"
                     /locus_tag="ROI21_000973"
     CDS             28116..28283
                     /gene="rmf"
                     /locus_tag="ROI21_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDX5109927.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(28353..28871)
                     /gene="fabA"
                     /locus_tag="ROI21_000974"
     CDS             complement(28353..28871)
                     /gene="fabA"
                     /locus_tag="ROI21_000974"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDX5109928.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(28940..30700)
                     /locus_tag="ROI21_000975"
     CDS             complement(28940..30700)
                     /locus_tag="ROI21_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDX5109929.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     AMEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPRADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANIR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            30886..31338
                     /gene="matP"
                     /locus_tag="ROI21_000976"
     CDS             30886..31338
                     /gene="matP"
                     /locus_tag="ROI21_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDX5109930.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELINRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(31413..32465)
                     /gene="ompA"
                     /locus_tag="ROI21_000977"
     CDS             complement(31413..32465)
                     /gene="ompA"
                     /locus_tag="ROI21_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDX5109931.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(32822..33331)
                     /gene="sulA"
                     /locus_tag="ROI21_000978"
     CDS             complement(32822..33331)
                     /gene="sulA"
                     /locus_tag="ROI21_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDX5109932.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            33550..34179
                     /gene="sxy"
                     /locus_tag="ROI21_000979"
     CDS             33550..34179
                     /gene="sxy"
                     /locus_tag="ROI21_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDX5109933.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(34142..36304)
                     /gene="yccS"
                     /locus_tag="ROI21_000980"
     CDS             complement(34142..36304)
                     /gene="yccS"
                     /locus_tag="ROI21_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDX5109934.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(36314..36760)
                     /locus_tag="ROI21_000981"
     CDS             complement(36314..36760)
                     /locus_tag="ROI21_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDX5109935.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            36883..38937
                     /gene="helD"
                     /locus_tag="ROI21_000982"
     CDS             36883..38937
                     /gene="helD"
                     /locus_tag="ROI21_000982"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDX5109936.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPAQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDGLLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(38969..39427)
                     /gene="mgsA"
                     /locus_tag="ROI21_000983"
     CDS             complement(38969..39427)
                     /gene="mgsA"
                     /locus_tag="ROI21_000983"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDX5109937.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(39523..40185)
                     /locus_tag="ROI21_000984"
     CDS             complement(39523..40185)
                     /locus_tag="ROI21_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDX5109938.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            40358..40771
                     /locus_tag="ROI21_000985"
     CDS             40358..40771
                     /locus_tag="ROI21_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDX5109939.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(40816..41133)
                     /gene="hspQ"
                     /locus_tag="ROI21_000986"
     CDS             complement(40816..41133)
                     /gene="hspQ"
                     /locus_tag="ROI21_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDX5109940.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(41191..42381)
                     /gene="rlmI"
                     /locus_tag="ROI21_000987"
     CDS             complement(41191..42381)
                     /gene="rlmI"
                     /locus_tag="ROI21_000987"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDX5109941.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            42476..42754
                     /gene="yccX"
                     /locus_tag="ROI21_000988"
     CDS             42476..42754
                     /gene="yccX"
                     /locus_tag="ROI21_000988"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDX5109942.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIH"
     gene            complement(42751..43080)
                     /gene="tusE"
                     /locus_tag="ROI21_000989"
     CDS             complement(42751..43080)
                     /gene="tusE"
                     /locus_tag="ROI21_000989"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDX5109943.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYQEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(43171..43830)
                     /gene="yccA"
                     /locus_tag="ROI21_000990"
     CDS             complement(43171..43830)
                     /gene="yccA"
                     /locus_tag="ROI21_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDX5109944.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(44037..44124)
                     /locus_tag="ROI21_000991"
     tRNA            complement(44037..44124)
                     /locus_tag="ROI21_000991"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44088..44090),aa:Ser,seq:tga)
     gene            44551..45669
                     /gene="hyaA"
                     /locus_tag="ROI21_000992"
     CDS             44551..45669
                     /gene="hyaA"
                     /locus_tag="ROI21_000992"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDX5109945.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            45666..47459
                     /gene="hyaB"
                     /locus_tag="ROI21_000993"
     CDS             45666..47459
                     /gene="hyaB"
                     /locus_tag="ROI21_000993"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDX5109946.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            47478..48185
                     /gene="hyaC"
                     /locus_tag="ROI21_000994"
     CDS             47478..48185
                     /gene="hyaC"
                     /locus_tag="ROI21_000994"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDX5109947.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            48182..48769
                     /locus_tag="ROI21_000995"
     CDS             48182..48769
                     /locus_tag="ROI21_000995"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDX5109948.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDEVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            48766..49164
                     /gene="hyaE"
                     /locus_tag="ROI21_000996"
     CDS             48766..49164
                     /gene="hyaE"
                     /locus_tag="ROI21_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDX5109949.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            49161..50018
                     /locus_tag="ROI21_000997"
     CDS             49161..50018
                     /locus_tag="ROI21_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDX5109950.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPALGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DGSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPS"
     gene            50152..51696
                     /gene="appC"
                     /locus_tag="ROI21_000998"
     CDS             50152..51696
                     /gene="appC"
                     /locus_tag="ROI21_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDX5109951.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            51708..52844
                     /gene="appB"
                     /locus_tag="ROI21_000999"
     CDS             51708..52844
                     /gene="appB"
                     /locus_tag="ROI21_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDX5109952.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENKLY"
     gene            52857..52949
                     /gene="cbdX"
                     /locus_tag="ROI21_001000"
     CDS             52857..52949
                     /gene="cbdX"
                     /locus_tag="ROI21_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDX5109953.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            53029..54339
                     /gene="appA"
                     /locus_tag="ROI21_001001"
     CDS             53029..54339
                     /gene="appA"
                     /locus_tag="ROI21_001001"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDX5109954.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAHSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACALHQDK"
     gene            complement(54327..54539)
                     /gene="cspH"
                     /locus_tag="ROI21_001002"
     CDS             complement(54327..54539)
                     /gene="cspH"
                     /locus_tag="ROI21_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDX5109955.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            54825..55037
                     /gene="cspG"
                     /locus_tag="ROI21_001003"
     CDS             54825..55037
                     /gene="cspG"
                     /locus_tag="ROI21_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDX5109956.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            55048..55236
                     /locus_tag="ROI21_001004"
     CDS             55048..55236
                     /locus_tag="ROI21_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDX5109957.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            55211..55441
                     /gene="ymcE"
                     /locus_tag="ROI21_001005"
     CDS             55211..55441
                     /gene="ymcE"
                     /locus_tag="ROI21_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDX5109958.1"
                     /translation="MRRWISQNNIRLPCGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            55431..55604
                     /locus_tag="ROI21_001006"
     CDS             55431..55604
                     /locus_tag="ROI21_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDX5109959.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(55652..56725)
                     /locus_tag="ROI21_001007"
     CDS             complement(55652..56725)
                     /locus_tag="ROI21_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDX5109960.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNNWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTAILISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLILGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(56797..59541)
                     /gene="torS"
                     /locus_tag="ROI21_001008"
     CDS             complement(56797..59541)
                     /gene="torS"
                     /locus_tag="ROI21_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDX5109961.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     DFDTTAIELQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTSVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQEMVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATEIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPTKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAHAIETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMETEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            59624..60652
                     /gene="torT"
                     /locus_tag="ROI21_001009"
     CDS             59624..60652
                     /gene="torT"
                     /locus_tag="ROI21_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDX5109962.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(60625..61317)
                     /gene="torR"
                     /locus_tag="ROI21_001010"
     CDS             complement(60625..61317)
                     /gene="torR"
                     /locus_tag="ROI21_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDX5109963.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            61447..62619
                     /gene="torC"
                     /locus_tag="ROI21_001011"
     CDS             61447..62619
                     /gene="torC"
                     /locus_tag="ROI21_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDX5109964.1"
                     /translation="MRKFWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTDTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            62619..65165
                     /gene="torA"
                     /locus_tag="ROI21_001012"
     CDS             62619..65165
                     /gene="torA"
                     /locus_tag="ROI21_001012"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDX5109965.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRSATAAQAAT
                     EAVTSKEGILTGSHWGAIRATVKEGRFVTAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEEFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            65162..65761
                     /gene="torD"
                     /locus_tag="ROI21_001013"
     CDS             65162..65761
                     /gene="torD"
                     /locus_tag="ROI21_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDX5109966.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIAVLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETCGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDNLRQKTLTA
                     LWQWLSEFAVRCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT        16299 a        16397 c        17403 g        15691 t
ORIGIN      
        1 cgcggagtac gtgggaacgg aatcacatca cgcacgtttt gcacgccagt tacgtaagca
       61 atcaggcgtt caaaaccaag accgaaaccg gaatgcggaa cagtaccgta gcgacgcaga
      121 tcgcgatacc accagtaatc ttctttgttc aggcccattt ccagcatacg ctcgtccagc
      181 acgtccagac gctcttcacg ctgggagcca ccaatgatct caccgatgcc cggagccaga
      241 acgtccatag ccgcaacggt tttaccgtct tcgttaaggc gcatatagaa cgctttaata
      301 tctttcggat agtttttaac cactaccggt gctttaaagt gttcttccgc cagataacgc
      361 tcatgctcag aagagagatc aacgccccag taaaccgggt tttcaaactt cctgccgcag
      421 ttttcgagaa tggtcactgc gtcggtgtaa tccacctgcg caaaatcggc ttcaatgaag
      481 cgttccagac gtgaaacggc atctttatct acgcgttcag cgaagaattt catgtcgtca
      541 gcgcgttctt cgagaaccgc tttgaagaca tatttcagca tggcttcagc cagacccgcg
      601 atatcgttca ggttagcaaa cgccacttcc ggctccagca tccagaattc cgccaggtga
      661 cggctggtgt tggagttttc agcacggaaa gtcgggccga aggtataaat tttggacaat
      721 gcgcaagcgt aggtttcgcc gttcaactgg ccagatacgg tcaggaaaga ctctttacca
      781 aagaagtctt tgtcgaaatc cactttgccc tgatcgttac gcggcaggtt ttccagatcc
      841 agcgtagaaa cgcggaacat ttcgccagcc ccttcggtat cagacgcagt aatcagtggc
      901 gttgaaaccc agaagaatcc ctgctcgtta aagaagcgat gcagcgcctg cgccagcgta
      961 tggcgaacgc gcgcgacggc accaatcagg tttgtgcgcg gacgcaggtg agcgacttca
     1021 cgcagatact caatgctgtg gcgttttgcc gccatcgggt aagtgtctgg atcttcaacc
     1081 caaccagcaa cttcaacctt gctggcctga atttcaaatt gttgcccctg gcccggcgat
     1141 gccacgactt tacccgtcac aatgaccgag caaccggtgg tcagacgcag gacgtcttca
     1201 ttgtaattgg gcagagaatt attgatgaca gcctgtacag gatcaaagca ggaaccgtca
     1261 taaacggcga ggaaggagat gccagctttt gaatctcggc gggtacgtac ccatccgcgc
     1321 acggtgactt cgctgtcaac ggctacgcgg ccctggagta cgtcggctac aggcacaacg
     1381 ctcataatat tctctctgtt aatagtcgga aaaaataaac acttgtccac ccgtaaatgg
     1441 ggggatacct atgttacctg gcatctgcaa tcagacaagc agaattcgca aatgcagcga
     1501 aagatttcgg aattaaaggt aaaaaaaggg agccgattag ctccctgatg atattaactg
     1561 gcttttttaa tatgcggaag gtcgaacgct ttgcgcagcg cccgaacaaa cgctttatcg
     1621 tggcagatag ttttgccagg gctgtcagaa agtttcgcca ctggtttacc gttacactct
     1681 accaacttaa tgacaatatt caggggtttt acctggggga tatcgcaggt caggcgagtc
     1741 ccaataccaa aacttaattg cacgcgggaa gagaagtggc ggtatagctc aaccgctttg
     1801 cgtaaatcca gattgtcaga gaaaaccagc gttttactct gtggatcaat tcccagcttc
     1861 tcataatgtg caatggcttt ttcaccccat tcaaccgggt cgccggagtc atgacgcagg
     1921 ccctgatagc gactggcgaa ctcgacaccg aaatcacgca ggaaagcatc catagtgatg
     1981 cagtcggtta atgcaatgcc aagttggtcg ggatattctt ccagccaggc agcaagtgca
     2041 gctcgctggc tgttggctag atccgggctg atttgctgat gcgcctggaa ccattcgtgt
     2101 gcctgtgttc ccatcggcgt gagggaaagc cgacgcgcca gatcgtagtt gctggtgccc
     2161 acgaaccagg attcctgttg cagacgtttc acgatggttt cttgtacttc acgagaaaaa
     2221 cgtcggcggg tgccaaaatc catcagatgg aagcgcgaca tatcaagacc ggcggttaat
     2281 gccgagaagt cggctaattt gctttccagc gtgtcgaggg cttgcgcaac gtcggcctgc
     2341 ggtgagcgat agcgatgtac catttcactg ataaccgcca gcaaaggaac ttcccagagg
     2401 atgacttcac gccacgggcc gcttaaacga atatccagct tgccattatc gttggacacg
     2461 gtgacttgtt ccgggttaaa gcggaactcg cgtagccagt taagatagtc ggccttaaag
     2521 aaaggcaggg ctgagagcca ttgatattca tcatcctgca ggcgcaggtg ctgcatcgcc
     2581 tgaacctgtt cacgaatagc atcggcataa atacccagca gatcgtcacc tcggcaacga
     2641 aactccgccg cgacatgcac atcgtaatag tgatgaaaca cggcttgctg catatgcaac
     2701 ttataagcat ctgtatccag caacgagtgc agaacaggag aagcgaattg tgtcataggt
     2761 gcgcagtagc gtcctcgtac aggagcgttt agtacaataa acatcttcag gaactgctgg
     2821 agtatacctt gtttcgcaat ttattgaacc ccgatcacac catatgccac ctttctggtc
     2881 gatggcatta cgcggtgcat gttataaaaa tgtagcaata aaggcgtttg tacctgaaaa
     2941 gatgaagatt ctgcatagcg cgatttacgc aacaggaata gactgaacac cagactctat
     3001 aaaagatgct aaaggttatt tatgactcaa cagccacaag ccaaataccg tcacgattat
     3061 cgtgcgccgg attaccagat tactgatatt gacttgacct ttgacctgga cgcgcaaaag
     3121 acggtcgtta ccgcggtcag ccaggctgtc cgtcatggtg catcagatgc tccgcttcgt
     3181 cttgatggcg aagatcttaa actggtttct gttcatatta atgatgagcc ttggaccgcc
     3241 tggaaagaag aagagggcgc actggtcatc agtaatttgc cggagcgttt tacgcttaag
     3301 atcgttaatg aaattagccc ggcgacgaat accgcgctgg aagggcttta tcagtcaggc
     3361 gatgcgcttt gcactcagtg tgaagccgaa ggtttccgcc atattacgta ttatcttgac
     3421 cgcccggacg tactggcgcg ttttaccacc aaaattattg ccgataaaac caaatatccc
     3481 ttcctgcttt ccaacggtaa ccgcgttgcg caaggcgaac tggaaaacgg acgtcattgg
     3541 gtacagtggc aggacccgtt cccgaaaccg tgctacctgt ttgcgttggt ggcaggcgac
     3601 ttcgacgtgc tgcgcgacac cttcaccacg cgttctggtc gcgaagttgc gctggagctg
     3661 tacgtcgatc gcggcaacct tgatcgtgcg ccgtgggcga tgacctcgct gaaaaactcc
     3721 atgaaatggg atgaagaacg cttcggcctg gagtatgacc tcgacatcta tatgatcgtc
     3781 gcggtggatt tcttcaatat gggcgcaatg gagaataaag gtctgaatat ctttaactcc
     3841 aaatatgtgc tggcccgtac cgacaccgcc accgataaag attacctcga tattgagcgt
     3901 gttattggtc acgaatattt ccacaactgg accggtaacc gtgtgacctg ccgcgactgg
     3961 ttccagctca gcctgaaaga aggtttaacc gtgttccgcg atcaggagtt cagctctgac
     4021 cttggttccc gcgcggtaaa ccgtatcaac aacgtgcgca ccatgcgcgg attgcagttt
     4081 gcggaagacg ccagcccgat ggcgcacccg atccgcccgg atatggtcat tgagatgaac
     4141 aacttctaca ccctgaccgt ttacgagaag ggcgcggaag tgattcgcat gatccacacc
     4201 ctgctgggcg aagaaaactt ccagaaaggg atgcagcttt atttcgagcg tcatgatggt
     4261 agtgcagcga cctgtgacga ctttgtgcag gcgatggaag atgcatcgaa tgtcgatctc
     4321 tctcatttcc gccgttggta cagccagtcc ggtacgccgg ttgtgaccgt caaagacgac
     4381 tacaatccag aaaccgagca gtacaccctg accatcagcc agcgcacgcc agctacgccg
     4441 gatcaggcag aaaaacagcc gctgcatatt ccgtttgcca tcgaactgta tgataacgaa
     4501 ggcaaagtga tcccgttgca gaaaggcggt catccggtca attcggtgct gaacgtcacc
     4561 caggcggaac agacctttgt ctttgataat gtctacttcc agccggtgcc tgcgctgctg
     4621 tgcgaattct ctgcgccagt gaaactggaa tataagtgga gcgatcagca actgaccttc
     4681 ctgatgcgtc atgcgcgtaa tgatttctcc cgctgggatg cggcgcaaag cctgctggca
     4741 acctacatca agctgaacgt cgcgcgtcat cagcaagggc agccgctgtc tctgccggtg
     4801 catgtggctg acgctttccg cgcggtgctg cttgatgaga agattgatcc ggcactggcg
     4861 gcagaaatcc tgacgctgcc ttccgtcaat gaaatggcag aactgttcga tatcatcgac
     4921 ccgattgcta ttgctgaagt acgcgaagca ctcactcgta ctctggcgac tgaactggcg
     4981 gatgagctac tggctattta caacgcgaat taccagagcg agtaccgtgt tgagcatgaa
     5041 gatattgcaa aacgcactct gcgtaatgcc tgcctgcgct tccttgcttt tggtgaaacg
     5101 catctggctg acgtgctggt gagcaagcaa taccatgaag caaacaatat gaccgatgcg
     5161 ctggcagcgc tttctgcggc ggttgctgca cagctgcctt gccgtgacgc gctgatgcag
     5221 gagtacgacg acaagtggca tcaggatggt ctggtgatgg ataaatggtt tatcctgcaa
     5281 gccaccagcc cggcggcgaa tgtgctggag acggtgcgtg gcctgttgca gcatcgctca
     5341 tttaccatga gcaacccgaa ccgtattcgt tcgttgattg gtgcgtttgc gggcagcaac
     5401 ccggcagcgt tccatgccga agatggcagc ggttaccagt tcctggtgga aatgctaacc
     5461 gatctcaaca gccgtaaccc gcaggtggct tcacgcctga ttgagccgct gattcgcctg
     5521 aaacgttacg atgccaaacg tcaggagaaa atgcgcgcgg cgctggagca gttgaaaggg
     5581 ctggaaaatc tctctggcga tctgtacgag aagataacta aagcactggc ttgataaata
     5641 accgaatggc ggcaatagcg ccgccattcg ggaatttacc cctgttttct caggcgagtt
     5701 tcagattcac cacgctgcat cacccgttgc aacacttccg cttccagctc cgctaatcgc
     5761 accgacccta accggcgcgg acgggggata tccaccgtca aatccagacc aatttttccc
     5821 tcttcaatta acaacacccg gtcagccatt gctaccgctt cgctcacatc atgcgtcacc
     5881 aacagtacgg taaagccgtg ctcctgccaa agtgacacaa tcaaatcctg catctcgagc
     5941 cgagtcaggg cgtccagcgc cccgagcggt tcatcaagca ataataaacc cggtcgatga
     6001 atcaacgctc ttgccagcgc cactcgctgt ttctgcccgc ctgaaagtgc ggcaggccat
     6061 tcccctgcgc gattctccag tcctactgca gccaacgccc gacgcgcggc atcgcgccac
     6121 tggcctttaa ggcctaaccc aacgttatca atcaccgatt tccacggcag cagacgcgca
     6181 tcctgaaaca tcattcgcgt gtcttcctga atctcagcca gcggtgtggt gcccgctaac
     6241 acatcgccag cggttggcgt ttccagacct gccagcaggc gcagcagggt acttttgcca
     6301 ccaccgctgc ggcccaccac cgccacaaac tgaccagccg gaatatgtaa atccagttgg
     6361 ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa tggtgtgccc
     6421 tggttcagac gagcagtatt cataccgtgg cctccttcaa atgataagcc gggttccagc
     6481 gcaaccagag gcgctctaac agctgcgcgc tgacgtcagc cagtttgccg agcagggcgt
     6541 aaagaataat ggcgactacc accacgtccg tttgcaaaaa ctcccgcgca ttcatcgcca
     6601 gataaccaat gccggaattg gcagaaatgg tttcggcaac aatcaacgtc agccacatca
     6661 ggcccaatgc aaaacgcacg ccgaccataa ttgagggcag ggcaccaggc aggatcacat
     6721 ggataaacag cggtatgccg gataatccat agctacgcgc catttccacc aacccgcgat
     6781 cgatattacg gatgccgtgc caggtgttga tataaatggg gaatagcgtg ccgagcgcca
     6841 ccagaaagat ttttgcggat tcatcaatgc caaaccacaa aatcaccagc gggatcagcg
     6901 ccagatgcgg cacgttgcgc aacatctgaa tggaggtatc cagcagccgt tctccccagc
     6961 gcgacagccc gctaatcagt cccagaatca agcccagcga tccgccaatt gaaaagccaa
     7021 tgagcgcccg ccaggagctg atcgccaggt gctgccacaa ttcgccgctg gcggagagcg
     7081 tccagaacgc cgtcaccact ccttccggag agggcaaaat acgcgtcgac agccagccaa
     7141 ctgaggaggc cagttgccac accgccacga tgcccaccgg taaaaaccag ggggcaacgc
     7201 gcaataacca ctttttcact ggcgttgcca ttatcgtgtt ccttagcttt gcgcgacttt
     7261 acgggggata aaatcatttg ccaccgcttc gccttgcgga ttcagcggct gcggctgggg
     7321 aatttccggg atggcgacat ccagatgcgg gaacagcaat tcgccaaccc gatacgcttc
     7381 ttccagatgc gggtagcccg aaagcacaaa actgtcgatg ccaagtgcag cgtattcgtt
     7441 gattcgcgcg gcgaccgtcg gaccatcgcc caccagcgcc gtcccggcac cgccgcgcac
     7501 taagccaacg cccgcccata aattggggct gatctccaga ttgtcgcgct tgccgttatg
     7561 taacgccgcc atccgctgtt gtcctacgga atccgtccgg gcgaatgcgg cttgtgcttt
     7621 ggcgatagtc tcatcatcaa gatgcgagat taaccgctcg gcggcctgcc acgcttcgtc
     7681 gttggtttca cgaacaatca catgcagacg aataccgaaa cgaattttgc gtccatgcgc
     7741 ggcagctttc gcccgcactt gttcgatttt ctctttaacc agttccggcg gttcgcccca
     7801 ggtgaggtag agatcaactt gttctgccgc cagctcctgg gcgacgtctg acgatccgcc
     7861 aaagtaaagc ggaggatacg gctgttgaat cggcgggaag agcagttttg ctccgcgcac
     7921 atgaatatgt ttgccgttga aatcgacggt ttctcccagc aataaacgcc gccaaacctg
     7981 ggtaaattcc gccgaggctt cgtagcgctc gctatgatca aggaacacgc cgtcgcctgc
     8041 cagttcttgt ggatcgctgc ctgtgaccag gttaaacaac gcacgaccat tcgagagacg
     8101 gtcaagcgtg gcggcctggc gggcggcaac ggtaggtgat gtcacactgg ggcgcagggc
     8161 gacaagaaac ttcagccgct gcgtcactgg gatcatcgac gccgccacca gccacgcatc
     8221 ttcgcaggag cgccccgttg ggatcagcac accggtatag cccaggcgat ccgccgcttg
     8281 cgcaatttgt tgcagataac cgtgatcaac ctggcgtgaa ccttcttccg ttcccagata
     8341 atgcccgtca ccgtgggtcg gtaaaaacca gaacatattc agactcataa ttgttttcct
     8401 tccagttgag tgggctgcca gatacgctgg cgaatatcga cttttttcgg caccagacgg
     8461 ttttcataaa acagatctgc cgtttgctgc tgtaaggcgg caacttcggc gttaaccggt
     8521 ttgatggtgg tgggagggcg atgatccaga tacgaggcaa tcaccggggc tggtaagccc
     8581 atcgtttttg ccaataaggc gatgctttgc tcgcgctggc tgcgggttaa cgcatcggcc
     8641 tcactaaagg ttgccagtac accctgaata aatgcgccgt ttttttctgc atacggacgg
     8701 gcagccagat aaaacgatcc ggtctgattg agatcggtgc cgtcttttag cacccgcacg
     8761 ccgccctgta ataatgcagc ggagtagtag ggatcccaga tagcccatgc gtcgacgtta
     8821 ccttgctgga acgcggcgcg ggcatcagcg ggtgtcaggt aagtgggttg tatatcggta
     8881 aacttaagcc cggcctggcg tagtgcgcgt agtaaaagat tgtgcgaact ggaacctttc
     8941 tgaaaggcaa ctttgtgacc tttaagatcg gctacggttt tgatcgggct gttttctgcc
     9001 accagaatca cttcggcttt gggcttcggc ggctcgacgc ccacgtatac caaatcagcc
     9061 ccggcggcct gagcaaagat tggcggaata tccccggtac tgccgagatc aatactgcca
     9121 acgtttaacg cctccagcat ctgtgggcca gccgggaact ccacccagga gatttttgtt
     9181 tgcggatagc gtttttccag catttggtaa tttttcgcca gcaccgaact gatgttgctt
     9241 ttctgatagc caatccgtaa cgcctctggc gaagactccg cggcaacagc aagtgtagag
     9301 acgctaagta atcccgccag cgctagttta atgatcttac gcatgggcat tacctcgcag
     9361 agacagaaga tcgggaacct gaacatcgcg gcggtgcaac gcctgccaga aagtttccag
     9421 cgcggtatca agacgggttt gcagatttgg cgtgaactgc ggcttgtggt ggtaatcaat
     9481 cacctgtgag tcatcggcaa acacgccgtg caggatctcc tgggctttca gcgcacttaa
     9541 aactggctta agggcataat cgaccgccag cagatgggcc accgtaccgc ccgtcgccag
     9601 cggtagcacc actttgcctt gcaacgcacg ttctggcagc aggtcgagca gggtttttag
     9661 cgcaccggaa taggcggctt tatacacagg cgtggcgaca atcagcccgt cagcctgttg
     9721 caactgttcg gtgaaggtat tgagtgccgg actatcgaaa cgagcataaa gcagatcttc
     9781 cggggcgaag ttttgcagat tccagtgata aacctctaca tccaggccat tcagtttttc
     9841 ccgcgcatat tccagcaagg agctggagcg agaaggaaag cgaggactac ccgccagggt
     9901 gatgacacgc atactctctc cttataacca attgttcttg ttttgttaac attgataaca
     9961 attcggtcag tctgtcggag agacaagaaa atgccaaata taaattttgt gtatcttttt
    10021 ctaaaaatga actaaggatt gagggatatg tatcacgtgt atttatttat tggatattca
    10081 ataaatgttg aatcaatgaa ttactcgttc aggtaataag taaatattca tgaaatctat
    10141 aaattaaaaa tttgtcactt attggattaa ggatgcttga tttaaatggc gggcaattgt
    10201 tgttattatt aagtccaatg tcatttaaag gcattatcat atttacgaag agatcaggat
    10261 gatgacgatg aaaaaaagtg tattgacggc gtttataact gtggtatgtg caacgtccag
    10321 cgttatggct gctgatgata atgctatcac ggatggctca gtaacattta atggtaaagt
    10381 tattgctcca gcttgtactc tggtcgctgc gacgaaagat tccgtggtga ctttgccaga
    10441 tgttagtgcc acgaagttgc aaaccaatgg tcaggtttct ggcgtgcaaa ctgatgtgcc
    10501 aattgaatta aaagattgtg atactaccgt aacaaaaaat gcaacgttca cctttaatgg
    10561 cactgcggat actactcaga ttacagcgtt tgctaaccag gcctcatctg atgctgctac
    10621 aaacgttgcc ctgcaaatgt atatgaatga tggtacaacg gccatcacgc cagacacaga
    10681 aaccgggaac attttgttgc aagatggaga tcagacgttg acttttaaag ttgattatat
    10741 cgctacgggg aaagcgactt ctggtaatgt gaatgcggta acaaatttcc atattaacta
    10801 ttattaatag aactcattaa ttgttttatt aattagtacc cctccagtgt tctggagggg
    10861 atattcatat tttttaagag tgactattta tgaaaacttg cataacaaag ggaattgtga
    10921 ccgtaagttt aacggcaata ttactcagct gctcatccgc atgggcggca ggtaaaggcg
    10981 ggattggact tgcagcaaca cgtcttgttt attcagaagg tgaagagcaa atttcactgg
    11041 gtgtacgtaa caccagtccg gatgttcctt atcttattca gtcatgggtg atgaccccag
    11101 ataataaaaa atcagcagac tttattatta caccaccgct atttgtgctg aatccggcta
    11161 atgagaatct gttacgcatt atgtacattg gtgcgccgtt ggcgaaagac agagaaaccc
    11221 ttttcttcac tagcgtacgg gcagtccctt caacaacgaa gcgggaagag ggaaataccc
    11281 tgaagattgc cacacaaagc gtcatcaaac ttttctggcg accaaaaagt ttagcgtatc
    11341 ccttaggcga ggctccggcg aaactgcgtt gcacttcgtc agctgacatg gttacggtca
    11401 gtaacccaac accttatttc attaccctga cagacctgaa aataggtgga aaagtagtta
    11461 aaaatcaaat gatttccccc tttgataaat accaatttcc tctgccaaag ggtgctcaaa
    11521 acagtagcgt aacgtaccga accatcaatg actacggggc ggaaacgccg caactcaact
    11581 gtaaatcgta agccgtcttc agttaagaga gcgagatgcc tagaactcac cgacaacaca
    11641 gcctgttaac ctctggtgga gtgccatcgt ttattggtgg gctggtggtg tttgtgtcgg
    11701 cggcgttcaa tgcacaagct gaaacctggt tcgatcccgc ctttttcaaa gatgatccct
    11761 caatggtggc cgatttgtcc cgtttcgaaa gagggcaaaa aataacgcca ggggtttatc
    11821 gtgtcgatat tgttctgaat cagacaatgg tagatacgcg caacgttaat tttgttgaga
    11881 taacgccaga gaaggggatt gccgcctgtt tgacgactga aagcctggat gcaatgggcg
    11941 tgaatactga tgcatttccg gcttttaaac aactggacaa acaagcgtgt gcgccattgg
    12001 cagagattat tccggatgcc agcgtaacct ttgatgtgaa taaactccgt ctggaaattt
    12061 cagtaccgca aattgctata aaaagtaacg cccgtggcta tgttccccct gaacgctggg
    12121 atgaagggat caacgcatta ttactgggat attcttttag cggggccaac agtattcata
    12181 gcagttcaga tagtgattcc acagatagct attttttgaa tttaaacagt ggcgtgaatt
    12241 taggtccgtg gcgattgcgc aataattcca catggagtcg tagtagcggg caaaccgcag
    12301 aatggaagaa tctcagcagc tatttgcagc gggcggttat tcctctgaaa ggcgaactga
    12361 ccgttggtga tgattatacc gcaggcgatt ttttcgacag cgtcagcttt cgtggcgtac
    12421 agctggcgtc agatgacaac atgctgccag acagcctgaa agggtttgcg cctgtggtgc
    12481 gtggtatcgc caaaagcaat gcccagataa cgattaagca aaacggctac accatttacc
    12541 aaacttatgt ctcgccaggt gcttttgaga ttagtgatct ctactccacg tcgtcgagcg
    12601 gtgatttgct ggttgaaata aaagaagctg acggcagcat caatagttac agcgtgccct
    12661 tttccagtgt gccattactc cagcgtcagg ggagaatcaa atacgcggtg acgctggcga
    12721 aatacagaac caatagtaat gatcagcaag aaagtaaatt tgctcaggcc acgctgcaat
    12781 ggggcggacc gtggggaacg acctggtatg gcggtggaca atatgctgaa tattaccgtg
    12841 ctgctatgtt ggggctgggt tttaacctcg gcgatttcgg agcgatttcg ttcgacgcga
    12901 cccaggcgaa aagtacactg gcagaccaaa gtgaacataa agggcagtca tatcgttttc
    12961 tgtatgccaa aacgctcaac catttgggca ctaactttca attgatgggc tatcgctatt
    13021 cgacgtcggg cttctacacc ctttccgaca ccatgtataa acacatggat ggctacgaat
    13081 ttaatgacgg tgatgatgaa gatacgccga tgtggtcgcg ttattacaat ttgttttaca
    13141 ccaaacgtgg caaactgcag gtcaatatct cccagcaatt aggcgattac ggttcgtttt
    13201 atttaagtgg tagccagcaa acatactggc ataccgatca acaggatcgg ctattacagt
    13261 ttggctacaa cacgcaaatt aaagatctct cactgggcgt ttcctggaac tacagtaagt
    13321 cccgtggtca acctgatgct gatcaagtat ttgcacttaa tttttccctg ccgctcaatc
    13381 tgttgctccc cagaagtaat gatagctata ccaggaaaaa aaattacgca tggatgacct
    13441 ctaataccag tatcgataac gaagggcaca ctacacaaaa tcttggttta acggagacac
    13501 tgctcgatga cggtaacctg agctacagcg tgcaacaggg atataacagc gagggggaaa
    13561 cggcgaatgg tagcgccagc atggactaca aaggggcgtt tgcggatgcc cgagtgggct
    13621 acaactacag cgataacggt agccaacaac aacttaacta cgccctttca ggcagtttag
    13681 ttgctcattc acggggcatt accctggggc aatctctggg ggaaaccaac gttctgattg
    13741 cagcaccagg cgcggaaaat actcgtgtgg cgaatagcac cgggctgaaa actgactggc
    13801 gcggatatac cgttgttccc tatgccactt cttatcgtga aaatcgaatc gtacttgatg
    13861 ctgcatcgtt aaaacgtaac gtggatcttg aaaatgcagt agtaaatgta gttcccacca
    13921 aaggggcgtt ggttctggcg gagtttaatg cccatgcggg gggaagggta ttaatgaaaa
    13981 catcaacgca gggtataccg ctgcgttttg gcgcgatagc gacgctggac ggcgtacaga
    14041 ctaatagcgg cataattgat gatgatggct cgctctatat ggcgggttta ccggcgaagg
    14101 gaacaataac cgtgcgctgg ggcgaagcac ccgatcaaat ttgtcatatc agttaccagc
    14161 ttaccgaaca acaaattaac gctgcgatta cgcgaatgga tgccatatgc agataatctt
    14221 tggagaaaaa tgcgtgtcat tactacgact attttttgcc gccgtcttaa tgctatggtg
    14281 cgctcaaacc gctgcttata gcgggcagtg tcataccact caggggaatc cctatattgg
    14341 cgtcaatttt ggcgttaaaa ccctggagga agaagaaaat actgcagggg tagttaaaga
    14401 caaattttat cagtggaacg aatcgaatga ttattatgtt tcctgtgatt gcgataaaga
    14461 caatgtcaga agtggccgat gggcattcgc cgcggattca ccgttagtct atttaggcga
    14521 caactggtac aaaattaatg actatcttgc cgccaaagtt ttattgcagg ttaaaggcag
    14581 ttctcctact gcggttcctt ttgaaaacgt gggcacaggg gcagataccc ggtggcatat
    14641 ttgtgacccc ggcggtcaac gtttaggcgg ccagggggct agcggtaata gcggtagctt
    14701 ttccctgaaa atattgcagc cgttcgttgg ttcggtcgtc attcctccta tggcgctggc
    14761 gcgattattt gaatgctaca acatacccgc aggtgattcc tgcacgacta caggtacacc
    14821 ggttttagtg tattacctgt ctggtacgat caattcactt ggctcatgtt ccgtcaatgc
    14881 cggagagaca atcgaagtcg atctgggcga cgtatttgcg gctaactttc gtgttgtagg
    14941 gcataagcct cttggggcca gaacggcaga acttgcagtc ccagtcaggt gtaacacggg
    15001 aaacgaaggg ttagttaacg tcaacctgag tctgacggca acctcagacc ccagctatcc
    15061 ccaggcgatt aagacgtcac gtcctggcgt gggcgttgtg gtgaccgata gccagaacaa
    15121 cattatttcc ccggcgggtg gaacactacc gctctctatt cctgatgatg cagacagtat
    15181 cgcgcgaatg aatgtctatc cggtcagcac gacaggcgta ccaccagaaa ccgggcgatt
    15241 tgaagccacg gcaacggtga gaataaattt tgattaacgc gtgaacgtat gaagaaaaaa
    15301 acaatatttc agtacgttat cttgttcttt agctttctta acacccatgt cgggatggct
    15361 gggcctgagc aagtcagtat gcatatttat ggtaatgtgg tcgatcaggg ctgtgatgtt
    15421 gccaccaaaa gcgcattaca aaatattcat attggcgatt ttaatatcag tgattttcag
    15481 gctgcgaata ccgtaagcac agctgctgat ttgaatatcg atatcaccgg ttgtgccgct
    15541 ggtattactg gcgcggacgt cctttttagc ggcgaggctg acacccttgc gccgacacta
    15601 ctcaaactaa ctgacacagg cggaagcggt ggtatggcaa cggggattgc cgtgcaaatt
    15661 cttgatgcgc aaagtcagca agaaatcccg ctcaatcagg tccagcctct tacgccctta
    15721 aaagcggggg ataacacact caaatatcaa cttcgctata agtccacaaa ggcgggggct
    15781 acgggcggta atgcgacggc ggttctctat tttgatctgg tttaccagtg aaggagtgat
    15841 gttgaaacgg ataatctgga ttctgtgctt actgggttta acggggagtt gtgagctatt
    15901 tgcccatgat ggcacggtca acatcagcgg atcgtttcgc cgtaatacat gcgtgctggc
    15961 acaggatagc aagcaaatca atgtgcagtt aggcgatgtc tcgctgacac gtttttctca
    16021 tgggaactat ggcccggaga aatctttcat cattaatttg caggattgcg gaacagatgt
    16081 cagcacggtt gacgttacct tttcaggaac acccgacgga atgcagagcg aaatgctgtc
    16141 cattgaaagt ggtacggatg cagccagcgg gctggcaatt gcgattctgg atgatgcgaa
    16201 aatattgatt ccgcttaacc aggcaagtaa agactacagc ttacatagcg gtaaggtgcc
    16261 attaactttt tatgcgcaat tgcgacctgt taatagcgat gtgcagtcgg gtaaagtgaa
    16321 tgccagcgcg acatttgtac ttcattatga ctaatacgtg gaacagattg acgctcgcga
    16381 tttttgccgt tttatcgctg ctggtggcgg ggaaattgca ggcaggagtg gtggttggcg
    16441 gaacgcgatt tatcttcccg gcagacagag aatcgatatc tatttcactg actaatacca
    16501 gtcaggaatc ctggcttatt aacagtaaaa tcaatcgccc aacgcgttgg gccggggggg
    16561 gaagcgtcga cagtgccagc accattactg gccgcgccgc cacttattct ccttaagccc
    16621 ggtactacag gcacattgcg cttgctgaga acggaaagcg acatcttgcc tgtggatcgc
    16681 gaaacgctat ttgagttaag cattgccagc gtaccatccg gcaaagttga aaatcagagc
    16741 gtaaaagtgg cgatgcgctc gatatttaaa ctgttctggc gacccgaagg tttgccgggc
    16801 gacccgctgg aagcttatca acaattacgc tggacacgga attcgcaggg tgtacaactc
    16861 actaacccaa cgccttacta cattaacctg attcaggtga gcgtaaatgg taaagcgtta
    16921 agtaatgcgg gagttgtgcc acctaaaagc cagcgtcaga caagctggtg tcagacaata
    16981 gcaccgtgtc atgtcgcctg gcggacgata aatgattacg gcgggttgag tgcaaagaag
    17041 gagcaaaatc tgctctgaaa caggctcgga aaacgtttgc gctttttttg ccacaggtca
    17101 attccctttt ggtccgaact cgcacataat acgcccccgg tttgcacacc gggaatccag
    17161 gagagttcat gtactacccc ttcgttcgta aagccctttt ccagctcgat ccagagcgcg
    17221 ctcatgagtt tacttttcag caattacgcc gcattaccgg aacgccgttt gaagcactgg
    17281 tgcggcagaa agtgcctgcg aaacctgtta actgcatggg cctgacgttt aaaaatccgc
    17341 ttggtctggc agccggtctt gataaagacg gggagtgcat tgacgcgtta ggcgcgatgg
    17401 gatttggatc gatcgagatc ggtaccgtca cgccacgtcc acagccaggt aatgacaagc
    17461 cgcgtctctt tcgtctggta gatgccgaag gtttgatcaa ccgtatgggc tttaataatc
    17521 ttggcgttga taacctcgta gagaacgtaa aaaaagccca ttatgatggc gtcctgggta
    17581 ttaacatcgg caaaaataaa gatacgccgg tggagcaggg taaagatgac tatctgattt
    17641 gtatggaaaa aatctatgct tatgcgggat atatcgccat caatatttca tcgccgaata
    17701 ccccaggact acgcacactg caatatggcg aagcgctgga tgatctctta accgcgatta
    17761 aaaataagca aaatgatttg caagcgatgc accataaata tgtgccgatc gcagtgaaga
    17821 tcgcgccgga tctttctgaa gaagaattga tccaggttgc cgatagttta gttcgccata
    17881 atattgatgg cgttattgca accaatacca ctctcgatcg ttctctggtt cagggaatga
    17941 aaaattgcga tcaaaccggt ggcttaagtg gtcgtccgct tcagttaaaa agcacagaaa
    18001 ttattcgccg cttgtcacag gaattaaacg gtcgcttacc gatcatcggt gttggcggca
    18061 ttgactcggt tatcgctgcg cgtgaaaaga ttgctgcggg ggcctcactg gtgcaaattt
    18121 attctggttt tatttttaaa ggtccgccgc tgattaaaga aatcgttacc catatctaat
    18181 tatttcttcg aattcctctt tataagataa tcagggcttt attttaagcc ctggttgttt
    18241 tatattcatc gctgttgctt atttagacat tttgtacttt tattgttgag gttattaagc
    18301 gaagcgacaa tggattgtgt tgctgcggtt ttatagggta ggggagaggc agatgcgaat
    18361 taaaccagac gataactggc gttggtatta cgatgaagag cacgatcgaa tgatgctcga
    18421 tttagccaat ggtatgctat ttcgctcacg ttttgagcgc aaaatgttga ctccagatgc
    18481 cttttctccc gcaggctttt gcgttgacga tgctgcgctc tatttctcgt ttgaagaaaa
    18541 gtgccgcgat tttaatttat ctaaagaaca aaaagccgaa ctggtgctga atgcactggt
    18601 agcaattcgt tatctcaaac cgcaaatgcc gaaaagctgg cattttgttt cccatggaga
    18661 aatgtgggtg cccatgccgg gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca
    18721 agtcaatttg ctggtcgttg aatctggcga aaatgccgcg ctatgtctgc tagcccaacc
    18781 ctgcgttgtt attgcgggtc gcgcgatgca gttgggcgac gccattaaaa tcatgaacga
    18841 caggctgaaa ccgcaggtga atgttgacag cttcagcctc gaacaggcag tctaacgcgc
    18901 caacttaagt gcagtcttcg gtacacagct acagcaaaga atggtgccat catcgcccat
    18961 tgctgatttt ttcagcggcg tgacttcgcc ttctaaaagc tgaacacggc aacttccaca
    19021 aatgcccgcg cggcaagaat aagggatacg aattccctga ttttctaatt gctccagcaa
    19081 cacctgctgg ttatttccac gaaatgcctg tccctgccag tcaatatcta catttgcgtc
    19141 cggttgttgc gtgatgttga cggtatcatc agcggcggct gcgccgtaaa ttttagccgg
    19201 agccgttgcc agaatttcca cctcatcgcc aatgcgaatt acgccactat tacgggcaat
    19261 taaattctgg ccaaaatcga catcgccgtt atcctgggca gtgcggaaag attgcaatgt
    19321 ttttaatggt tcgcctgccg gatgtttttg ccctttttct gggctgacgg tggtgaaaat
    19381 acagcggcta caaggtttaa ccacatcaaa caccacatca ccaatacgaa tcactttcca
    19441 gctatcttct tcccaggctg acgcgccgga aaccaccaga ttagggcgga attgctccat
    19501 ttttacactg gccggacaac gttgttggag atcacgtaac gaggcttcgt tagcaagaag
    19561 gtaaggatag ccatcagcaa atgacagggg tacagtgttg tggcgtttca cgcgccgcgt
    19621 catttgtggc cccacccagc gcaactgcac ttcgcgggag aaaaatccac ttagccattt
    19681 gttgatcgcg tctggcgcaa ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc
    19741 gtcttgtgtg gcgaaatcag caaaacgaac atatgcgcta ctgccatctg gtgcggtgag
    19801 atgcaagcca tcatgcacag gtgaaggagt aaaccgtacc atttgcggaa actggcgagc
    19861 ggtaataaac gtaccgtcag gttccgtgat cataaagatg cgatcgaagg ccagaccact
    19921 gacatctgcc agagcatgtg taagaccaat gccgcgcatc gatttaacag gatgaataaa
    19981 aagccgggtt aatgtcgcca cggtgcggtc ctcaaatgaa aataagccct caactttatg
    20041 acatgacgcg cttattagct ataatgcgca acaattttct tagcgcctgt cccattaggc
    20101 tattttattt gtcagtttgg cccagggaag tgctcgaaat cctcacgtac ttgtgtacgc
    20161 tccggtttct ccgcgctgtc catgtccaga ctgactgcaa caattacgcc tactgcggta
    20221 ggctcttaga gtaaaagtga cgatatgaat tctctgtttg ccagtacggc ccgtgggctg
    20281 gaagagctgt taaaaactga actggaaaac ctgggggccg ttgaatgcca ggtggttcag
    20341 ggtggggtcc atttcaaggg cgacacacgg cttgtttacc agagcctgat gtggagccgc
    20401 ctggcctcgc gtattatgtt gccgctgggc gagtgtaagg tttacagcga tttagacctc
    20461 tatctcggtg ttcaggcgat caactggaca gagatgttta atcctggcgc gaccttcgct
    20521 gtccacttca gtggtttgaa tgacaccatc cgcaacagtc agtacggtgc gatgaaagtg
    20581 aaagacgcga ttgtcgatgc tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc
    20641 gatgcgccgg atatccgcgt taacgtctgg ctgcataaag aaaccgccag tatcgccctt
    20701 gatctcagtg gtgatggttt acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg
    20761 atcaaagaaa ccctggcagc cgctattgtg atgcgatccg gctggcagcc aggaacaccg
    20821 ctgctcgatc cgatgtgtgg ttccggtacg ttgctgattg aagcggcgat gctggcgacc
    20881 gatcgcgcac caggcttgca ccgtgggcgt tggggcttta gcggctgggc gcagcatgat
    20941 gaagctatct ggcaggaagt gaaagcggaa gcgcaaactc gcgcccgtaa aggtctggct
    21001 gagtacaact ctcatttata cggttccgac agcgacgcac gggtgattca acgtgcgcgc
    21061 actaacgccc gtcttgcggg gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg
    21121 caactgacca atccgctgcc gaaagggccg tacggtacag tgttgagcaa cccgccatac
    21181 ggtgaacgtc tggacagcga accggcgctg attgcgctgc atagtctgct gggccgaatc
    21241 atgaaaaacc agttcggtgg ctggaatctc tctttgttta gtgcctcgcc ggatctgtta
    21301 agttgcctgc agttgcgtgc agacaaacag tacaaagcga aaaacggtcc attagactgc
    21361 gtgcagaaaa attaccacgt tgccgaaagc acaccagaca gcaaaccggc aatggtggca
    21421 gaagactacg ccaaccgtct gcgtaagaac ctcaaaaaat tcgagaagtg ggcgcgtcag
    21481 gaaggcattg aatgctaccg cctgtacgac gccgatctac cggaatacaa cgttgcagtt
    21541 gaccgttatg ccgactgggt ggtggtgcag gagtatgcgc cgccaaaaac tattgatgcg
    21601 cacaaagcgc gtcagcgtct gttcgatatt atcgctgcaa ccatttcggt actggggatt
    21661 gcgccaaaca aactggtgct gaaaacccgt gaacgccaga agggcaaaaa tcagtaccag
    21721 aaactgggcg agaagggcga gtttctcgaa gtcaccgaat ataacgctca cttgtgggtg
    21781 aacctgacgg attacctcga taccggtctg ttcctcgatc accgtatcgc ccgtcgtatg
    21841 ctcggtcaga tgagcaaagg caaagatttc ctcaacctgt tctcttatac cggcagcgcc
    21901 accgtgcacg cgggattagg cggtgcacgc agcaccacca ccgtggatat gtcgcgtact
    21961 tatctggagt gggcagaacg caacctgcgt ctgaatggcc taaccgggcg tgcgcatcgc
    22021 ctgattcagg ccgattgcct ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc
    22081 tttatcgatc caccaacctt ctctaactca aaacgaatgg aagatgcgtt tgatgttcag
    22141 cgcgatcatc tggcgctgat gaaagatttg aaacgtctgc tgcgtgctgg tgggacgatc
    22201 atgttctcga acaacaaacg tggcttccgt atggatcttg acggactggc aaaactggga
    22261 ctgaaagcac aagaaattac gcaaaaaacg ctctcccagg atttcgcccg aaaccgccag
    22321 atccacaact gctggctgat taccgcagcc tgaaaggaat agtaatgtca ttaatcagta
    22381 tgcatggcgc atggctgtcg ttcagcgacg cgccgcttct cgataacgca gaactgcata
    22441 tcgaagataa cgaacgtgtt tgtctggtag gccgcaacgg cgcaggcaaa tcgacgttaa
    22501 tgaaaatcct caaccgtgaa caagggctgg atgacggtcg cattatttac gagcaagatt
    22561 tgattgtagc tcgtctgcaa caggatccgc cgcgtaacgt tgaggggagt gtttatgatt
    22621 tcgttgccga aggcattgaa gaacaagcgg aatacctgaa acgctatcac gatatttcgc
    22681 gcctggtgat gaacgacccg agcgagaaaa atctcaacga actggcgaag gttcaggaac
    22741 agttggatca ccacaacctg tggcagctgg aaaaccgcat taacgaagtg ctggcgcaac
    22801 tggggttaga tcctaacgtt gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg
    22861 cattaggacg cgcgctggtg agcaatccgc gcgtattgct gctcgacgaa ccgactaacc
    22921 acctggatat cgaaaccatc gactggctgg aaggattcct taaaaccttc aatgggacga
    22981 taattttcat ctcccacgac cgttcgttta tccgcaatat ggcgacgcgc attgttgatc
    23041 tcgatcgcgg caagcttgtg acctatcctg ggaattacga ccagtacctg ctggaaaaag
    23101 aagaagccct gcgcgtggaa gaactacaaa atgccgagtt cgatcgcaaa ctggcgcagg
    23161 aagaggtgtg gatccgccag gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac
    23221 gcgccctgaa agcaatgcgt cgcgagcgtg gcgaacgtcg cgaagtgatg ggtaccgcaa
    23281 aaatgcaggt ggaagaagcc agtcgctccg gtaagatcgt tttcgaaatg gaagacgttt
    23341 gctaccaggt tgacggtaag caactggtga aagatttttc ggcccaggtt ctacgtggcg
    23401 acaaaattgc cctgattggc ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc
    23461 tcggtcagct tcaggcggac agcgggcgta ttcacgttgg caccaaactg gaagtggctt
    23521 atttcgatca gcaccgtgcg gaactggatc ccgataaaac ggtgatggat aaccttgctg
    23581 aaggtaagca agaggtgatg gttaacggca agccacgcca tgttttgggc tatttgcagg
    23641 acttcctgtt ccatccgaaa cgggcaatga cgccggtacg tgcgctttct ggcggtgagc
    23701 gtaaccgctt gttgttggcg cgtttgttcc tcaaaccaag caacttattg attcttgacg
    23761 aaccgaccaa cgatcttgat gttgaaacgc tggaactgct ggaagaactg atcgacagct
    23821 accagggcac ggtattgctg gtgagccacg atcgtcagtt tgtcgataac accgttacag
    23881 aatgctggat cttcgaaggc ggcggtaaaa ttggtcgtta tgtcggcggt tatcatgatg
    23941 cccgtggtca gcaagaacag tatgtggcgc tcaaacagcc tgcggtgaaa aaaatcgaag
    24001 aagccgccgc gccaaaagca gaaactgtaa aacgcagcag tagcaaacta agctataaat
    24061 tgcagcgcga actggagcag ctaccgcaat tgctcgaaga tctggaggcg aagctggaag
    24121 ccctacagac gcaggtggcg gatgcttcct tcttcagtca gccccatgag cagacacaaa
    24181 aagtgctcgc cgatatggct gctgcagagc aggagctgga gcaagccttt gaacgctggg
    24241 agtatcttga agcgttaaaa aatggtggct gatcgcaaaa tagtcgattt aaacagagac
    24301 agctgacaac ggtaaaattg cctgatgaac tgggtttatc aggcatgaaa accaacactt
    24361 tgttagtcat ctgtaaaata gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg
    24421 ccgcgaagca catcctgtgc tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc
    24481 atggtcagaa agcggcatgt ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc
    24541 ctcggcagcg tcccaccgcc tatgcgttgg ctgcactgtt catgctgttg ctgtccaact
    24601 tgtttccttt tgtgaatatg aacgttgcag gagttaccag tgaaattaca ttactggaaa
    24661 ttcccggcgt gcttttttct gaggactacg ccagcctcgg cacctttttc ctgttgtttg
    24721 tgcaactggt tcccgcgttt tgtctgataa ccattctgtt actggtgaat cgcgcggaat
    24781 taccggtccg tttaaaagag caactggcac gggtgctttt tcaactcaaa acctggggaa
    24841 tggcggagat tttcctcgcg ggtgtgctgg tcagtttcgt taaactgatg gcctacggca
    24901 gcattggtgt cggcagcagc tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg
    24961 cctttcagtg cgttgatcgt cgctggttat gggacgatat cgccccgatg ccagaactgc
    25021 gccagccgct aaaacctggc gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt
    25081 gtacggcaat ccttcctgct gacgaacccg tgtgcccgcg ttgtagtacc aaagggtacg
    25141 ttcgacgtag aaacagcctg caatggacac tcgcgctgct tgttacgtcc atcatgctgt
    25201 atcttccggc taatattttg cccatcatgg tgacggattt attaggctcg aagatgccat
    25261 cgacgattct cgctggggtc atcctgttat ggagcgaagg ctcttacccc gtcgctgcgg
    25321 ttatctttct ggccagtatt atggtgccaa cgttaaagat gatcgccata gcgtggttgt
    25381 gttgggatgc caaagggcat ggcaagcgcg acagtgaaag aatgcatttg atttatgaag
    25441 ttgttgagtt tgtaggccgc tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg
    25501 cgctggtgcg tatgggaggt ttaatgagta tttatccggc aatgggtgca ttaatgtttg
    25561 ctttagtcgt cataatgaca atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg
    25621 atcgtcaacc tgaatcagag catgaggagt cctgacagta tggaatctaa taatggggaa
    25681 gccaaaatcc agaaagtgaa gaactggtct cccgtgtgga tatttcctat cgtcacggcg
    25741 ctcattgggg cctgggttct tttttatcat tacagccatc aggggccgga agtgaccctg
    25801 atcaccgcga atgcggaagg aatagaaggt ggtaaaacca ccattaaaag ccgtagcgtt
    25861 gacgtcggcg tggttgaaag cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa
    25921 gcgcggctga attccggtat ggaaaaattg ctgcataaag ataccgtctt ttgggtggtt
    25981 aaaccgcaga ttggtcgaga agggattagc ggcctgggaa ctctgctgtc tggtgtttat
    26041 atcgaactgc agccaggcgc gaaaggcagc aaaatggata aatacgattt gctggactca
    26101 ccaccgttgg ccccgcctga tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc
    26161 gggcagctct cgccaggaga tcccgtgctg ttccgtggct atcgggtagg ttcggttgaa
    26221 accagcacct tcgatacgca aaaacgcaat atcagctatc aactgttcat caatgcacct
    26281 tatgaccgac tggtgaccag caatgttcgc ttctggaaag atagtggcat tgcggttgat
    26341 ctgacgtcag cggggatgcg tgtggagatg ggctcattga caacgctgct tagtggcggt
    26401 gttagctttg atgtgccgga aggtctggat ttagggcagc cagtggcacc gaaaacagct
    26461 ttcgttttgt atgatgatca gaagagcatt caggattcgt tgtacaccga tcacattgat
    26521 tatctgatgt tctttaaaga ttcggtacgc ggcctgcaac cgggagctcc ggtagaattc
    26581 cggggtattc gccttggtac cgtaagcaaa gtgccattct ttgcaccgaa tatgcgtcag
    26641 acatttaacg atgattaccg tattccggta ctgattcgta tcgagccaga gcggctgaaa
    26701 atgcagcttg gcgaaaatgc ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt
    26761 ttacgcggat cgctgaaaac cggaaacctg gtcactggcg cactgtatgt tgatctcgat
    26821 ttctatccaa atacgcctgc aataaccggt attcgtgaat ttaatggcta tcagattatc
    26881 ccgaccgtta gcggcggtct ggcgcaaatc caacaacgac tgatggaagc gttggataag
    26941 atcaacaaac tgccattgaa tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt
    27001 cagcgcacaa tgaaaaacct gcaaacgacg ctggatagca tgaacaagat cctcgccagc
    27061 cagtcgatgc agcagctgcc gatggatatg cagtcaacgt tgcgtgaatt gaatcgcagc
    27121 atgcagggct tccagcctgg ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc
    27181 cttgatcagg tgttgcgaga actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac
    27241 gcgctggtat ttgaagcgaa ggacaaaaaa gatccagagc cgaagagggc gaaacaatga
    27301 aaaagtggct agtgacgatt gcagcactgt ggctggccgg atgcagctcc ggcgaaatta
    27361 ataaaaacta ttaccagtta cctgtggtgc agagcggtac acaaagtacc gccagccagg
    27421 gtaatcgcct gttatgggta gagcaggtca ctgttcctga ctatctggcg gggaatggtg
    27481 tggtttatca aaccagtgat gtgaagtatg tgattgccaa caacaacttg tgggccagcc
    27541 cgttggatca acagttgcgc aacactctgg ttgccaacct gagcacgcaa ctgcccggct
    27601 gggtggttgc ctcccagcct ctgggaagcg cccaggatac gctcaatgtt accgtaacgg
    27661 agtttaacgg gcgatatgat ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc
    27721 agggacaact gatcaaacgt ccgttccgtc tggaaggggt gcaaactcag gatggttacg
    27781 atgaaatggt taaagtgctg gccagtgtct ggagtcagga agccgcttct attgcgcaag
    27841 agataaagcg tctaccgtaa tcataaagat ttgtaaatat aaccgtctcc ggtatgttgc
    27901 ctgaggcggt ttttttgttt ctaacttgcg gaaaaagttg ttcctcttca cattttttgt
    27961 acaactgaca tgcccgtgta gctcacaaat atgacagtgg cgtgaatttt gcgcattgac
    28021 ggcagttatg attcgcggta ttgcttaact gtgattgcac atttagtaat cactgttttc
    28081 ttttccacca gaaaccagta tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc
    28141 tggaacgggc acatcaacgt ggttatcagg ccggcatcgc cggacgctca aaagaaatgt
    28201 gtccctatca gacgctgaat caaaggtcac aatggctggg aggctggcga gaagccatgg
    28261 cggacagggt agtaatggcc tgattctgtc tctttaaaaa gaaacctccg cattgcggag
    28321 gtttcgcctt ttgatactct gtctgattat aatcagaagg cagacgtatc ctggaacaga
    28381 ccgactttca ggtcgctggc ggtatagatc agacgaccat caaccagcac ttcgccatcc
    28441 gccaggccca taatcagacg acggttaaca atgcgtttaa agtgaatacg gtaggtcact
    28501 tttttcgctg tcggcagtac ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg
    28561 cctttacctt cgccgcccag ccagccgagg tagaacccta ccagctgcca cattgcgtcc
    28621 aggcccagac atcctggcat aaccggatca ccaatgaagt ggcatccgaa gaaccacaga
    28681 tccggattga tatccagttc tgcttcaaca taccctttgt cgaagttacc acccgtttcg
    28741 gtcattttga ccacacggtc catcatcagc atgttcggtg ctggcaattg cggaccttta
    28801 gcgccaaaca gttcaccgcg accagaggca agaaggtctt cttttgtata ggattcgcgt
    28861 ttatctacca tgttctctgt aagccttatt ttattgaagc acgcaggata gctaacacgt
    28921 gtacgctgaa caagtccgat cagttcggaa taaaccagtt cagccaacgt aatggccatg
    28981 gaaaacggtg acgtccttcc tgttgcgatg cttgcgcgat acgttcctgg atggtttgca
    29041 tcagcgtcgt ttggccttcg ccatcccaca ccagatttaa taataacggc agtgcgtcag
    29101 ttacatcgtc tactgcccag atggtgaatt tgccttcttc taccgctttc accagttcac
    29161 tgtgaagact taaatggcga atgttagcag tggggataat gacaccttgt ttcccggtta
    29221 actcacgttg ctggcaaata gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg
    29281 gctgggcacg accgaactga tcgactgaac ctgtgatagc gatactctga ttcaccggca
    29341 catcggcgag ggcgcttatc agggcgcaga gttcagccat cgaggcacta tcgccatcca
    29401 cttcactgta tgactgctca aatgtcagcg atgctgagaa ggggatctgt tgctcaagct
    29461 gtagttccga catcaggaac gcctgcatga tcatcatccc tttcgcatgg atattgccgc
    29521 caagctccgc tttgcgttcg atgtcggtga attcaccatc gccaatatgc acaacgcagc
    29581 taatgcgaga aggttcgcca aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg
    29641 cgttaatttg cccgatgcgt tcgccttcgg tttcaatcag gatttgctcc tgaagaatct
    29701 catcctgcat gcgttcagcg aggaaacctt cgcgccattc acgctgctgt agcattaagt
    29761 ttagctgctc gccggagaag gtgtcgccat cgcacaggga ggcgacctct ttacactggc
    29821 ggaggatcca ctgcgggcta agcggaagtg tttcttgttc accggtgtag cgtgctgctt
    29881 cgcggataag caccggccag gcatccgctc tcggtgcagg taagtgatta tgtctggcgg
    29941 tcaatgttac ccagcgacac cactggctta ctgactccgc atcgacaatc tgcagagtat
    30001 cttcaaattc gctataaata gcctgctctg aaagctctgg ttccatctcc tggaaatcag
    30061 ccaatgattc gcgttcgcct accagaatga ctttcagctt caatggcatc gaaggcacag
    30121 agacggggag agggcgcgac tcatcaaacg caacccagtc aaaacgctcg cggttaacga
    30181 tatttttcag ccgcatccac agcagaggtt gcgccagcag tgtacgcaaa gagatgatga
    30241 gaataccgcc atttgcctga tgcaccagac ctggctgcag ggtaatgtcg ccattaaact
    30301 ggcgcaggca gccaaagagt tgttccgctt ctacccagtc ggcagcgaca acttgcgtta
    30361 aagtcgcaaa attatcatct gcactcactg cgttacgtaa gcggatggtg tgcccggaaa
    30421 cctcatagtg accgcccacc agttggcctg catcgctttg tagcgcacgc gcggcatcgg
    30481 caataagatt cagatactca gactcttccg gggccttcgc cagcataaaa gaggaggatg
    30541 ctcgcgtatg cagcaactgc tccatcgcaa attgcagtcg cggttgagta tcactgaata
    30601 aaggatcgtt ttcgtcaatc aaatgtggct gagcaaatat ttcctgatag ctatcggtat
    30661 caggaaccag gtcacgccat gcaagtttcg taatggtcaa agttgatgtt ttttagtctg
    30721 ttgtcaaagc cgcgattata ccgtaaccgg cactgcagca cacgtagaaa gcaccgacaa
    30781 tactcctggc atgggcgtta aagctcacag gatggagatt cttttcttca ctggcccaaa
    30841 aagctgatat tctgtaaaga gttacacggt aacattgaga tcgctatgaa atatcaacaa
    30901 cttgaaaatc ttgaaagcgg ttggaaatgg aagtatctgg tgaagaagca tcgcgaaggg
    30961 gagttaatca cccgttacat agaagccagt gccgcccagg aagccgtcga tgtgttgctc
    31021 tcgctggaaa atgaacccgt gctggtaaat ggctggattg acaagcatat gaatccggaa
    31081 ctgatcaatc gcatgaagca aaccattcgg gccagacgga agcgccattt taatgcggaa
    31141 caccagcata cgcgcaaaaa atcgatcgat ctggaattta ttgtctggca acgtctggct
    31201 ggtcttgcgc agcgtcgcgg taaaacgctt tctgaaacga ttgttcagct gattgaagat
    31261 gcggaaaaca aagagaaata cgcgaataaa atgtcttctt tgaagcagga tctgcaggca
    31321 ttgctgggta aggaataact gacgaaagtc agttcaattt tctatagacg aaaaaaaacc
    31381 ccgcagcagc ggggttttta ccagacgaga acttaagcct gcggctgagt tacaacgtct
    31441 ttgatgcctt taacttcgat ctctacgcga cgatccggag ccaggcagtc gatcagtgca
    31501 gcacgctgtt tcacgttgtc acaggtgttg ccagtaaccg ggttggattc gcccatacca
    31561 cgtgcggaga ttttgtctgc cgggatacct ttagagatca ggtaatcaac aacagactga
    31621 gcacggcgct cggacagacc ctggttgtaa gcgtcagaac cgatgcggtc ggtgtaaccc
    31681 agaacaacta cggaaccgtc tttcggatcc aggttgctca gctggctgta cagctgatcc
    31741 agagcagcct gaccttccgg tttcagggtt gctttgttga aggtgaacag aacgtcagac
    31801 ttcagagtga agtgcttggt ctgtacttcc ggtgccggag ctggagccgg agcaactact
    31861 ggagctgctt cgccctgacc gaaacggtag gaaacaccca ggctcagcat gccgttgtcc
    31921 ggacgagtac cgatggtgtg tgcgtcaccg atgttgttgg tccactggta ttccagacgg
    31981 gtagcgattt caggagtgat cgcatactca acaccgccag cgaagaccgg agaaacgccg
    32041 gtatcgtggt ctttaaaaga tgcgccacca ggtacgttag ccttggtgtc tgcacgccat
    32101 accataccac ccagacgagt gtagatgtcc agatcgtcag tgattgggta acccagttta
    32161 gcggtcagct gaacgccctg agctttgtat gcgccgttga tgttgtcgcc tttgtacggc
    32221 atacgaccta accagtcgta acccatttca aagccaacat acgggttaac ctggtaacca
    32281 ccaaaagcac ctgcacccag ttggttttcg tgggtcggac cattgttagg aataaaacca
    32341 gtgtcatggt actgggacca gcccagttta gcaccagtgt accaggtgtt atctttcgga
    32401 gcggcctgcg ctacggtagc gaaaccagcc agtgccactg caatcgcgat agctgtcttt
    32461 ttcatttttt gcgcctcgtt atcatccaaa atacgccatg aatatctcca acgagataac
    32521 acggttaaat ccttcaccgg gggatctgct caatattaac tctaccgata tcttcggctt
    32581 atgccgagca cccctggcga tgtaaagtct acaacgtagt tgaaaactta caagtgtgaa
    32641 ctccgtcagg catatgaaaa aaaagtcttg tataaggtac gtttaatctt ttttgtcagc
    32701 gacaatttac agaagaaaat cgcggaaacc gcttcagaca agcctccgca aggaaaatta
    32761 gtcacgactg aaagcattgg ctgggcgaca aaaaaagttc caggattaat cctaaattta
    32821 cttaatgata caaattagag tgaattttta gcccggaaag ttgtctcgtt gcgtgagagg
    32881 atgcgcttac cggacgcata ataaacccca tagcgttacc ttcatttgcc gcatcaacaa
    32941 gttcagcatg ctcttcttca gtcaaatcat ctgccaacca accgatcacc acgctgtaat
    33001 tgcccgtgcg taaagcgcga accattgact ccacggtgtg gcaaggggag agctggctaa
    33061 tctgcattac tttcgttaag ggtagcccag atgcctgaac ccattcgcga ctcagttttt
    33121 gttgcggtgt taaccaaagt tgccagcgcg attgctgacc gagttgctgt aacaatggca
    33181 acagtagaag ttgcgtcatc atgggctgat cttcgcgata gacaacttca ctgataagcc
    33241 cggctgtagt gttttccgta gagacacgcg caattttact tgctgcggat gagaacgacg
    33301 aagaacgatg tgcatagcct gaagtgtaca taatcaatcc agcccctgtg agttactgta
    33361 tggatgtaca gtacatccag tgacaacaaa gatcaaccct attttcggaa agagcctcgc
    33421 aaattttgtc gttggtgacg ggaaaacata aattaatctt gccccttaag aataagttgc
    33481 ctattttcgt agttaacgga tccgttaatg tgaatcattc ttttatgtta tgattttaaa
    33541 aggaatttta tgaaaagcct ctcctataag cggatctata aatcacaaga atacctggca
    33601 acgttgggca caattgaata ccgatcattg tttggtagtt acagcctgac cgttgacgac
    33661 acggtgtttg cgatggtttc tgatggtgag ttgtatcttc gggcttgtga gcaaagtgca
    33721 cagtactgtg taaaacatcc gcctgtctgg ctgacatata aaaagtgtgg ccgatccgtt
    33781 accctcaatt actatcgggt tgatgaaagt ctatggcgaa atcaactgaa gctggtgcgt
    33841 ctgtcgaaat attctctcga tgcagcgctg aaagagaaaa gcacgcgcaa tacccgggaa
    33901 agactgaaag atttgcccaa tatgtctttt catctggaag cgattctcgg ggaggtgggg
    33961 attaaggatg tacgggcgtt acgtatactt ggggcaaaaa tgtgttggtt gcgactgcgg
    34021 cagcaaaaca gtctggtgac agaaaagatt ctgtttatgc ttgaaggggc cattatcggc
    34081 attcatgaag ctgcgctccc ggtggcacgc cgccaggagc ttgcagaatg ggctgactct
    34141 cttacgccga aacaggagtt tcctgcggaa cttgagtaat ctggcgttgc agacgaccaa
    34201 tctctggcag taatgcaatc aataacccaa cttgttgtac gaccagaggt tctttgctgt
    34261 ctgcccgagg ttcaagttgc tgcatccgct gtttcaggcc agctaatgcc tgattgacgc
    34321 gttcttcatc agcaggttga tgatgtagcg cgtcatcaac atagcaaacg gcgtcatcaa
    34381 gaaacgccag aatttcagga ttagttaact gctcccggtg agcaccgagg gctgagatat
    34441 agctggtaaa cgtatggtta aggcacagca accgaaacgc ggcttcgcga atttgcgggg
    34501 taacgttcgg ttcgctggac atatttgata ccaccgacgc cagttcagca tcacggttgt
    34561 gtgcatcgcg gcgggcaata cgatacgcca gacggttatc acgcccctga tggtattgtt
    34621 ccagtatggc atcgagatac cgacagttgg cctctgtggc gcgttcgagc attcgcggca
    34681 gattgcgaaa cttccagtca ggccagatgt agctcaccgc cgcccacgca atggcacaac
    34741 caatcagcgt atcgattacg cgaggtaacg ccacttcaaa accttcaccc agcaagttaa
    34801 aacacagtag caccaaaagt gtgatgaaca tcgttgcatg ggcgtattgc acattacgga
    34861 aggcaaaaaa gagcacgccg gtaataacca gcagcaccag ctgcccttcc agtgatggca
    34921 caaaccacag cacaggaatg ccaattgcga tacctaccag cgtaccaata atccttaact
    34981 tcaggcggtg gcgcgtggcg ttatagtttg gctggcagac aaacaaactt gtcagcaaga
    35041 tccaataccc gtgatgcatt ccggttatct gaatgatggc gtagccgaag cacaacacca
    35101 gcgacattct taccgcatga cggaagaggg cggattccgg cgtgaagtga cggctaagac
    35161 gcagccagat atcactcaat ccgtgcgggc tgtcatcagc gagctcattt tcgtcattat
    35221 tatggggcag tgcctgggcc tgttctgatt caattgttgc cagttgggca tcaatagcgc
    35281 gtaaattgtt cagcaaaaat cccagtgttt tgagtaaatc ggcaggtgcg ccgttatcgc
    35341 gcatccgctc cagcgcagca tcaatatgcg taaaagcgcg ctcgaaatgc ggatcatgtt
    35401 gataaggctg acgcaacaaa atacagcgtg acagttgctg gcacgcctgg ccctgcatcg
    35461 acatcagccg ctgaaaacgg aacagcacgt cgctgtggcg aaaatgttca cgcaatgttt
    35521 gatactgaat atgagaagag ctggcacgct cgtgaatatc ctgcgcgacg aagtaataat
    35581 gcagcgtgcg acgcgttccc cgttgaccac gatcgccacg taagcgggtc agcagcgaga
    35641 gtttcgtctg attcaatgtc gccatcagct gaccgttggc gagagccaaa tcgtacagcg
    35701 gtgcctggct ttcatcttca atatcaggat caaacatgcg cgacttgagc tcaaggtaac
    35761 gcgccagttg ttcatagcaa cgcgccaggt tgtcctgcag cgggcggacc gggaacagta
    35821 gatgaccaat aagtgtcagg acgttgtacc agacggcacc ggccagcaga tacatcggct
    35881 gctgatacca gtgctcatac agtgacgtcc ccaacatagt gtaaatggcg atcagtaacg
    35941 caccgaaggc aattgttgca tagcgttgac ccagaccgcc gagcaaaatg aagccgctgg
    36001 ttgagagcgt taagccaatc gcaaatagcc agggccaggg aaacagcagt tctactgagg
    36061 ccgaggcgat aaaaaagcag aacagcgtaa tgatgaggtt acgcaaacgt cccgccagcc
    36121 ggtcatcgag atcggtcagc gccgctgcca ccatccccag cgtcagcgga atcgtcagtt
    36181 ttacatcacc cagccaccac ggaaacgctg tggttccaca aagcgcaata aaaatacgcg
    36241 cgtaatacag ccaggcgctg ttccaggtat agcgtttgag caaaggactt agcataaagg
    36301 ccatattagt ggattattca aaacgacgac gtgcattggc ttcacgcgca gcttgcgctg
    36361 tttctaccga taccacgcga cgaccgaccg gccatagtgc aatagcggca attttaaagt
    36421 tcgcaatgcc gacaggaatg ccaatgatgg aaatacattg tgcgatgccc gttgcaatgt
    36481 gcatcaggca taaccaccag ccaaagaaaa tcagccagaa aatattcaat accgtaccgc
    36541 cagtattcag cagcacattt ttgccagccg ggttcagttc atcgacatga atagcctcat
    36601 tgccataagg caccagagac agtttagtaa tctcccagca ggatcgtgtc agcggtaagg
    36661 taaaaatcag cacaatactg accagagtcg ccaacagcca gcccagagtg gtggcaaatc
    36721 cgccaagcac aaagttcaga atgttcaaaa cggtacgcat aaaacctcgc tttactgtgg
    36781 ttttcagtaa tgggcggcaa ttgtaacgtt tttttgggct ggagcacgtt ttctctgacg
    36841 gttacactga taagaaatca tttcgtgtgg atctacagag tcatggaact gaaagcgaca
    36901 acgcttggaa aacgtctggc acagcaccct tacgatcggg cggtgatcct caatgccggg
    36961 attaaagtct ccggcgatcg ccacgaatac cttattcctt tcaatcaatt actggcgatt
    37021 cactgtaagc gcggtctggt atggggcgag ctggagtttg tactgccgga cgaaaaagtg
    37081 gtgcgtctgc acggcaccga atggggcgag acgcagcgtt tttaccatca tcttgatgct
    37141 cactggcggc ggtggagtgg cgagatgagc gaaattgcgt ctggtgtttt acgccagcaa
    37201 ctggatttga ttgccacgcg tactggagaa aataaatggc tgacgcgtga gcaaacctct
    37261 ggcgtgcagc aacaaatccg ccaggctttg tcggcgttgc cgttgccggt taaccgactg
    37321 gaagaattcg ataactgccg tgaggcgtgg cgtaaatgtc aggcctggtt gaaagatatt
    37381 gaaagcgctc ggttgcagca taatcaggcg tataccgaag ccatgcttac cgagtatgcg
    37441 gattttttcc gccaggtcga gtcttcaccg ctgaatccgg cgcaggcccg ggcagtcgtt
    37501 aatggcgagc attctttgtt agtgctggct ggtgcaggaa gcggaaaaac gtcggtgctg
    37561 gtggcccgtg caggctggtt gctggcgcgt ggtgaagcgt cccctgcgca aattttattg
    37621 ctggcgtttg gtcgcaaagc cgctgaagag atggacgagc gtattcgcga acggctacat
    37681 accgaagaca ttaccgcacg cacgtttcat gcgctggcgc tgcatattat tcagcagggc
    37741 agcaaaaaag ttccgatagt cagcaaactg gaaaatgata ccgctgcccg tcatgaactc
    37801 tttattgctg agtggcgcaa gcaatgcagc gaaaagaaag cgcaggcgaa gggctggcgg
    37861 caatggctga cggaagaaat gcagtggtca gtgccagaag gtaacttctg ggatgatgaa
    37921 aaattacagc gtcgtcttgc ctcacgcctc gatcgttggg taagtctgat gcggatgcac
    37981 ggtggtgcac aggcagaaat gattgccagt gcacccgaag agattcgcga tctgttcagt
    38041 aaacgtatca agttgatggc cccgttatta aaagcctgga aaggtgcgct gaaggcagaa
    38101 aacgctgtcg atttttcggg ccttattcat caggcgattg tgattctgga gaaaggtcgc
    38161 tttatcagcc cgtggaagca tattctggtt gatgaatttc aggatatctc gccgcagcgg
    38221 gcagcgttgt tagcggcatt acgcaagcaa aacagtcaga cgacgttgtt cgccgttggt
    38281 gatgactggc aggcgattta ccgattcagc ggtgcgcaaa tgtcgctcac caccgctttc
    38341 catgaaaact ttggtgaagg cgatcgctgt gacttagaca cgacttaccg ttttaacagt
    38401 cgtatcggtg aggtggcaaa ccggtttatt cagcagaacc caggccagct gaaaaagccg
    38461 ctaaacagct taaccaatgg agacaaaaaa gccgtcacgt tattggatga gagccatctt
    38521 gacggtttgc tggataagct ctctggttat gccaaaccgg aagagcgcat tctgatcctg
    38581 gcgcgttacc atcacatgag gcctgccagc ctggaaaaag cggcaacacg ctggccgaag
    38641 ttgcaaatcg actttatgac cattcatgcc agcaaagggc aacaggcgga ttatgtcatt
    38701 atcgttggct tgcaggaggg aagtgatggt tttccggctg cggcgcggga gtcgattatg
    38761 gaagaggcgc tactgccacc ggttgaggat ttcccggacg ctgaagaacg gcggttaatg
    38821 tacgtggcgc taacccgggc acgccatcgg gtatgggcac tgtttaacaa agagaatccc
    38881 tctccctttg tggaaatact gaaaaatctg gatgtgccgg tggcgagaaa accgtaagga
    38941 acaggtggcg tttgccacct gtgcaatatt acttcagacg gtccgcgaga taacgctgat
    39001 aatcggggat cagaatatcg accgcgtcgt tgaaatgcgg cgactggata atgaagtctg
    39061 ccgttgccac gttggtggcg accggaatgt tccataccgt cgccagacgc aacagggctt
    39121 tcacgtcagg atcgtgcggc acggcattta gtgggtccca gaagaaaatc aatacatcaa
    39181 ttttcccttc tgagatcaat gcgccaacct gctggtcacc ccccattggg ccactcaaca
    39241 tcgcgttgac gttcatgccg gttgcacggg aaatcaagtt accggtagtg cctgttgcat
    39301 acagtacgtg ttgttccagt aacggttgat gccgttccac ccagctcatc agcatttgtt
    39361 tgcagtgatc gtgtgccacc agcgcaatat gtttccgcgc aggtaaagtg cgagtcgtca
    39421 gttccataat gtacatccgt agttaacttt cctacagatt actgtaagca cttatcgctg
    39481 caagataaag accgaaaaag cctgcgcaca ggcacaaaaa tctcaggaag atggttgttt
    39541 ttccgcccat tgcaggaaag tatttcgcgt ttgtgggtca gccagtttaa accaatactt
    39601 caaccgttgt tctgtgagca cctgagactg cggtggaata gcatccagct cggaaacacc
    39661 cgaaagcagc gtactgtcat ctgccatcat cgtggcaaat tgcggtagtg aagcgcgttt
    39721 cgcggatttg ttatagcgtt caacctcgac ctcataatca atcgtttttg ccgttgaggt
    39781 aatggcgaga atatccagct ttaccggaat cggcgtcgca tcgccatcca acaattcaag
    39841 gcgcggcgcg gcatcaaaat ggttagcttc ccgctcattc tccaggcgag gcaggcgaaa
    39901 attgacctgg ttgatgagct gggtgttaaa actgaccacc agcggtgggg agatatacag
    39961 ccgttcttca ctattggaaa gatgaatcgt cttctcaacg cgaaacacta actgatgcgg
    40021 tccgttatcc agttcaatgc tgtccgcacc gcgcagcaga gagctggaaa ccttcttgcc
    40081 gtcgagtacc agaagatcga catcggttga aagccgcagc gtggtggcaa aaacggatac
    40141 cggcaaacat aatgcaatca aggtggtcac gatgccggtt ttcataggta gctcctgtca
    40201 aaagaccgcc cgaataataa tgtgtcaaaa tttttcttta atcatactta ctaacatata
    40261 gacatatttc ccgcttttgc tctcattcat tcgtattagc tgcatggttg gcatgtcggc
    40321 tttgtcgtac actttgttaa acagtctgga ggaaataatg aaagaaaccg atattgcagg
    40381 cattttaacg tccactcaca ccattgcgct ggtgggggca agcgacaaac ccgatcgtcc
    40441 cagctatcgc gtgatgaaat atctactcga ccaggggtat cacgtcattc cggtttcgcc
    40501 aaaagttgcc ggcaaaacac tgctgggcca gcaggggtat ggcacgctgg cagaagttcc
    40561 tgaaaaagtc gatatggtcg atgttttccg gaattcagaa gcggcgtggg gcgtagcgca
    40621 ggaagctatt gccattggcg cgaaaaccct ctggatgcaa ctgggggtga ttaatgagca
    40681 agcggcagta ctggcacggg atgccgggtt aaatgtagtg atggatcgct gcccggctat
    40741 tgagatccct cgcctgggcc tggccaaata aaaaatcccc ggaaggcaaa aaccttccgg
    40801 ggatttgttc agggattagt tacgcagacg cggggcctgg agttgtttgc ggatggtctg
    40861 cgccagctca tccatggatg gctgttcagg atgttcatcc tgcaattcgc tacttagctg
    40921 ggcttcagcc aggtaggtat gaaccggtag gccgttatcg tcctccatca ccacgtgata
    40981 ccacggagcg gcgcgaagct cgtcattcac cgccagctca tcaggcgacg gttcagagag
    41041 cgaataaacc gggtcgatat ccacgaccac tccgagataa cctaacaggg aatggcggac
    41101 ctgctggccg ataccgaatt tgctggcaat catagtcacc tcccgggaaa tctttacctt
    41161 tgatataagg gtaaaattcc acatttcaag ttacatgacg cgacaggcaa accctttcag
    41221 atatagccct tccggatagg tagcgatcac cggatgatcg gctgcctgac ggaactgctc
    41281 tataaattgt acatcacgac cggcatcaat tgcggcatcc gcgatgattt tctgaaataa
    41341 atcgctggtc atcagaccgg aacaggagaa agtcaggaga ataccgcctt cattcagcaa
    41401 ctgaatcgcc agcatgttga tatctttata gccacgacac gcgcccatca actggctttt
    41461 attctcaaca aatttcggcg ggtccatcac gataacgtca aatttttcac cgcgatcgcg
    41521 ataagtacgc agcaatttaa agacatcatc acggacaaac tcagccttgc tcagatccag
    41581 tttgttcagc tcaacgttct gccgtgcaat atccagggct tcctgggagg tatcaacgct
    41641 gacaacctgg ctgcaaccgc ccatcagtgc cgatacggcg aaaccaccgg tataggagaa
    41701 acagttcagt acgcgtttat tttcaacgta gcggcgggta gccaggcggc tatcacgctg
    41761 atccaggtag taacccgttt tgtgtccgtg ctgaatatcc accagcagct tcatgccgtg
    41821 ttcttcaatc ggcagcaggg caggcggcaa ctcgccggtg acggggccct gggtcagctc
    41881 catcccttct tttttacgta ccgcaacatc gctgcgatcg taaatcgcac attccgggta
    41941 cagcgtttgc agggcgctaa ttaatgccgc gcgctgatat tctgcgccag cactcagtag
    42001 ttgcagcacc agaaaattac cgaaacgatc gatagtaata cccggcaggc catcagattc
    42061 accggcgatt aaacgatagc tgtcgaggcc atctttttgc gccagccagt cacgccattt
    42121 ttgtgcttgt tgcaaacggc gggaaaaaaa agcaatgtcg atagactcag acgggtcaaa
    42181 cgtccagacg cgtgcccgga tttgcgaagc tggcgaataa gcgccgcgtg ctaaccattt
    42241 tccctgatga tcaacaatat cgatggtttc accgaggctg gctttacctt ccatgcgggc
    42301 aacggccccg gaaaagaccc acggatggcg acgaagtaat gatttttcgc gccctttggc
    42361 taacactaaa cgtacactca taatttactt ttctgtcgat gcaaagaaat gggcgtcatt
    42421 gtccggagtt ttccatggat ttgcaacgcg tccattaagg ataaggagag caacaatgtc
    42481 gaaagtctgc ataattgcct gggtttacgg gcgagttcag ggcgtaggat ttcgctacac
    42541 cacgcagtac gaagcgaaaa gactgggctt aaccgggtac gccaaaaatc ttgacgatgg
    42601 cagcgtggaa gtggttgcct gcggtgaaga agggcaggta gaaaaattaa tgcagtggtt
    42661 aaagagtggc ggcccgcgtt ctgcgcgtgt agaacgggtg ctcagcgagc cgcatcatcc
    42721 ctcgggggaa ttaaccgatt ttcgtatcca ttaaatacat tttaccggtt ttggcaggcc
    42781 agcaattttg gttgcttgct tagccggacc tttcggaaac agtcggtata aatagcggct
    42841 gttacctttt tcttcgccaa atttattcgc catcgctttt accagcatac gaatcgccgg
    42901 agacgtatta aattcctggt agaagtcacg tacaaaacgc accacttccc agtgttctgg
    42961 cgacagcgaa atcccttcgt tctctgcaat caccactgcc agcggctcac tccactggct
    43021 gctttctttg agatagcctt cggtatccgt ttctatctct ttaccttcga agatcagcat
    43081 aattattact accttaatca gactggcggc agtgtaacaa aaaacaaagc cccgcataaa
    43141 gcgaggctcg gtaagtaacc ctgggcgaga ttaatcgcgg ctggcgaagc ccagaatgct
    43201 cagcaggctg acgaagatgt tgtacagcga aacatacagg ctaactgtgg cacgaatata
    43261 gttcgtttca ccgccatgaa tgatgttgct ggtttcaaac aaaatagcgc cagaggagat
    43321 cagaatgaag accgcgctga tcgccagatg cagagcaggc agttgcagga agatattagc
    43381 aaccatacca atcagcacca ccacaatacc cgccatcagc ataccgccaa ggaatgacat
    43441 atctttgcgg gtggtcagca catatgcaga acagcagaag aacactaacg ccgttccgcc
    43501 cagtgccata gcgattacgt cacccattcc ggcagacaga taggtgttca gaattggtcc
    43561 gaggatataa cccagaaaac cggtaaaggc gaatgcggag ataatcccgg tcggcttatt
    43621 cgccgtttta taggtcagga acatcaaacc atacataccc accagcgtca gaatcagacc
    43681 cggagatggc agcatcagca ccgtactggc agttgcggta atcgcagaaa aggccagcgt
    43741 caggctcagc agaaaatagg tattacgcag caccttatgg gtgctaagca gcgatgtacg
    43801 gtcatgtgaa gaactaacaa tacgatccat tgagtcactc tctatgacag atgtaattaa
    43861 ttaagcagca taatgataat gcgtaagggc acccagaagt tttacccatc tttacgcatt
    43921 tgatctggaa caggtttaac agcggattat caggtcatta agcaaatata acgccctgag
    43981 aatttcgaca ggcaaaagaa aaaggggtta gcatttagct aaccccttat cttatttggc
    44041 ggaagcgcag agattcgaac tctggaaccc tttcgggtcg ccggttttca agaccggtgc
    44101 cttcaaccgc tcggccacac ttccggaatg acgcgcacta taaacatccc gatgcggcgt
    44161 gtaaacccct aatttgtttg tttgcctgaa aaacagccaa aagtgcattg atagcgtgaa
    44221 ataacagcag attgatcatt tcatcaccat gaattccttc tcttttactc gtttagcaac
    44281 cggctaaaca tccccactgc ccggcaaaaa gaaaaatagg tccattttta tcgctaaaag
    44341 ataaatccat acagtttgta ttgttttgtg caaaagtttc actacgcttt attaacaata
    44401 ctttctggcg acgtgcgcca gtgcagaagg atgagctttc gttttcagca tctcacgtga
    44461 agcgatggtt tgccttgcta cagggacgtc gcttgccgac cataagcgcc cggtgtcctg
    44521 ccggtgtcgc aaggagaaga gacgtgcgat atgaataacg aggaaacatt ttaccaggcc
    44581 atgcggcgtc agggcgttac ccggcgcagc tttctcaaat attgtagtct ggctgccacg
    44641 tcgctgggat taggcgcggg aatggcacca aagattgcct gggcgctgga gaacaaaccg
    44701 cgcattccgg tggtatggat ccacggtctg gaatgcacct gctgtaccga atcttttatc
    44761 cgctccgctc acccgctggc aaaggacgtc atcctttccc tgatttccct cgattacgac
    44821 gatactttga tggctgccgc cggaacccag gcggaagaag tctttgaaga catcatcacg
    44881 caatacaatg gcaaatatat cctcgcagta gaaggtaatc cgccgctggg cgagcagggg
    44941 atgttctgta tcagcagcgg tcgaccgttt attgagaaac tcaaacgtgc cgctgccgga
    45001 gccagcgcga ttatcgcctg gggaacctgc gcgtcctggg gctgcgtgca ggccgcgcga
    45061 cccaatccga cgcaggcaac gcctatcgac aaagtcatca ccgacaaacc tattatcaaa
    45121 gtacctggct gcccgccgat cccggatgtg atgagcgcca tcattactta catggtgacc
    45181 tttgatcgct tgccagatgt cgacagaatg ggccgtccac tgatgtttta tggtcagcgt
    45241 atccacgata aatgttatcg ccgcgctcac ttcgacgccg gagagttcgt ccagagttgg
    45301 gatgatgacg ctgcccgcaa aggttactgc ctgtacaaaa tgggctgcaa agggcctacc
    45361 acctataacg cctgttcctc cacacgctgg aacgatggcg tttccttccc gatccagtca
    45421 ggtcacggct gcctgggttg tgcggaaaat ggtttctggg atcgcggttc gttctacagc
    45481 cgcgtggtcg atattccaca aatgggtact cattccaccg ccgacaccgt cggtttaacc
    45541 gcgcttggcg tggtggcagc ggctgttggt gtgcacgcag tcgccagcgc cgttgaccag
    45601 cgcagacgtc ataaccagca acctacagaa accgaacatc agccaggcaa tgaggataaa
    45661 caggcatgag cactcagtac gaaactcagg gatacaccat caataatgcc ggacgccgcc
    45721 tggtggtcga cccgattacg cgcattgaag gccacatgcg ctgcgaagtg aatattaacg
    45781 atcagaatgt gatcaccaat gccgtctcct gcggcaccat gtttcgcggg ctggagatca
    45841 tcctgcaagg gcgtgacccg cgcgatgcgt gggcgttcgt tgaacgtatc tgcggcgtct
    45901 gtaccggcgt acacgctctg gcttcggtct atgccatcga agatgctatc ggcattaaag
    45961 tgccggacaa cgctaatatc atccgcaaca ttatgctggc gacgctctgg tgccatgacc
    46021 atctggtgca cttttatcag cttgccggga tggactggat cgatgtgtta gatgcgctga
    46081 aagccgatcc gcggaaaacc tccgaactgg cgcaaagcct ctcctcatgg ccgaaatcat
    46141 cccctggcta tttcttcgac gtacaaaatc gcctgaagaa attcgttgaa ggcgggcagt
    46201 tggggatctt ccgcaatggc tactgggggc acccgcagta caaactgccg ccagaagcca
    46261 acctgatggg ctttgcccac tatctcgaag ctctcgattt ccagcgtgaa attgtcaaaa
    46321 tccacgcggt ctttggcggt aaaaacccgc atccaaactg gattgtcggc gggatgcctt
    46381 gcgccattaa cattgacgaa agcggcgcgg tcggggcggt caatatggaa cgcctgaacc
    46441 tggtgcagtc gattatcacc cgcacggcgg atttcattaa caacgtgatg atccctgacg
    46501 ccttagccat tggtcagttc aacaaaccgt ggagcgaaat cggcacgggt ctttctgata
    46561 aatgtgtcct cagctacggc gcattcccgg atattgccaa cgactttggc gagaaaagtc
    46621 tgctgatgcc tggcggcgcg gtgattaacg gcgacttcaa caatgtgctg ccagtggatt
    46681 tggttgatcc gcagcaggtg caggagtttg tcgaccacgc ctggtatcga tatcccaacg
    46741 atcaggtcgg gcgtcatccg ttcgatggca tcaccgaccc gtggtacaac cccggcgatg
    46801 tcaaaggcag cgataccaac attcagcagc tgaatgaaca ggaacgctac tcgtggatca
    46861 aagcgccacg ctggcgcggt aatgcgatgg aagtggggcc gctggcacgc acgttaatcg
    46921 cttatcacaa aggcgatgcg gcgaccgttg agtcggtcga tcgcatgatg tcggcgttga
    46981 acctgccgct ttccggcatc cagtcaacgt taggccgcat tttgtgtcgc gcgcacgaag
    47041 cgcagtgggc cgcaggcaag ttgcaatatt tcttcgacaa gctgatgacc aatctcaaaa
    47101 acggcaatct cgccaccgcc tctactgaaa aatgggaacc agcaacctgg ccgacagagt
    47161 gccgtggtgt cggttttacc gaagcgccgc gcggggcgtt aggccactgg gccgccattc
    47221 gcgatggcaa gattgatctc taccagtgcg tggtaccgac cacctggaac gccagcccgc
    47281 gcgatcccaa agggcaaatt ggcgcttatg aagcagcgtt aatgaacacc aaaatggcga
    47341 tccccgagca accgctggag atcctgcgta ctctgcacag ctttgacccg tgcctcgcat
    47401 gttcaacaca cgtgctgggc gacgacggta gcgagctgat ctccgtgcag gtgcgttaac
    47461 agcgaaggag aatcatcatg caacagaaaa gcgacaacgt tgtcagccat tatgtctttg
    47521 aagcgccagt gcgtatctgg cactggctga cggtgttatg catggcggtg ttgatggtca
    47581 ccggatactt tatcggcaag ccgctacctt ccgtcagcgg cgaggcgacg tatctgtttt
    47641 acatgggcta catcaggtta attcacttca gcgccgggat gatttttacc gtggtcttgc
    47701 tgatgcggat ctactgggct tttgttggca atcgttactc ccgcgagctg tttatcgtgc
    47761 cggtatggcg taaaagctgg tggcagggcg tgtggtatga aatccgctgg tatctgtttc
    47821 tggcaaaacg tccgagtgcc gatataggcc ataaccccat cgcccaggcg gcgatgttcg
    47881 gctatttcct gatgtcagtt tttatgatca tcaccggctt tgcgctgtac agcgaacaca
    47941 gccagtacgc tatttttgcg ccgttccgtt atgtggtgga atttttctac tggacgggcg
    48001 gcaactcaat ggacattcac agctggcatc ggctggggat gtggctgatt ggcgcgtttg
    48061 tgatcggtca tgtctacatg gcgctgcgtg aagacatcat gtccgacgac acggtgatct
    48121 ccaccatggt caacggctac cgtagccaca aatttggcaa aataagtaac aaggagcgtt
    48181 catgagcgag caacgcgtgg tggtaatggg gctgggcaac ctgctgtggg ccgatgaagg
    48241 cttcggcgtg cgggtggcgg aacggctgta tgcccattac cactggcccg atgaagtcga
    48301 gattgttgat ggcggcactc agggactgaa cttgctgggg tatgtcgaaa gcgccagcca
    48361 cttgctgatt ctcgacgcca ttgattacgg gttggaaccc ggaacgctgc gaacatatgc
    48421 cggagaacgc attccggctt atctcagcgc gaagaaaatg agcctgcacc agaacagctt
    48481 ctccgaagtg ctggcgctgg cggatatacg tggtcatctg ccagcacata ttgccctcgt
    48541 cggtctgcaa cccgcgatgc tcgacgacta cggcggcagc ctgagcgaac tggcacgaga
    48601 gcaactgccc gctgctgaac aatcggcgct ggcgcagttg gctgcgtggg gaattgtgcc
    48661 gcaaccggct aatgaatcgc gttgtctcaa ttatgactgt ctgtcgatgg aaaattacga
    48721 aggcgttcgc ttgcgccagt accggatgac acaggaggag cagggatgag caacgacacg
    48781 ccatttgatg cgttgtggca acgaatgctg gcgcgcggct ggacgccggt cagtgaattc
    48841 cgtcttgacg actggcttac gcaagcgcca gacggcgtgg tgttattaag cagtgacccg
    48901 aaacgcacgc cagaggtcag cgataatccg gtaatgattg gcgaattact gcgcgagttt
    48961 cccgactaca catggcaggt ggcgattgct gaccttgagc agagcgaagc catcggcgat
    49021 cgctttggcg tctttcgctt tcccgccaca ttagtgttta ccggcggaaa ctatcgcggc
    49081 gtgctgaatg gtattcaccc gtgggcggaa ctgataaacc tgatgcgtgg gcttgtcgaa
    49141 ccacaacagg agcgtgcctc atgagcgaaa cttttttcca tctgctgggg ccaggaacgc
    49201 aaccgaacga tgacagtttc agcatgaatc cactgccgat cacctgtcgg gtgaatggtg
    49261 aaccgagtat ggcggccctg gagcaatgtg ctcacagccc gcaggtgatt gcgctgttaa
    49321 acgagttaca acatcaacta agcgaacgcc agccggcgct gggtgaggta ctggcagtcg
    49381 atctgttaaa tctcaacgcc gacgatcgtc actttatcaa tacgcttctc ggggaagggg
    49441 aagtgtcagt gcgcattcag caggctgatg gtagtgaaag tgaaattcag gaggcgatct
    49501 tctgcggatt atggcgggtg cgcagacgtc gcggcgacaa gttgctggag gacaaactgg
    49561 aggctggctg cgctccgctg gcattgtggc aggcggcaac gcaaaacgtc ttgccgacag
    49621 attcgctgtt accgccgccc attgatggcc tgatgaatgg cctaccgttg gcgcatgagt
    49681 tactggcgca tgtacgtaac cccgacgcgc agccgcacag cattaatctg acgcaattac
    49741 ccatcagcga ggctgatcgg ctttttctct cacgtctctg tgggccggga aatattcaga
    49801 ttcgcaccat tggctacggc gagagctata tcaacgccac ggggttacgc catgtctggc
    49861 atttacgctg cacggacacc ttaaaaggcc cgttacttga gagttatgaa atctgcccga
    49921 taccggaagt ggttctggca gcgccagaag atttggtcga ctcagcgcag cggcttagcg
    49981 aggtatgtca gtggctggcg gaaggggcac cttcgtaaaa agacggtaag tatcgctttc
    50041 aggcttatga atatcgcaat cggcgaatac ctctggtcgt agagtttcag gataaagagg
    50101 gagatctacc attaacgggt tatttttctc tcttcgccta caggagtgcg catgtgggat
    50161 gtcattgatt tatcgcgctg gcagtttgct ctgaccgcgc tgtatcactt tttatttgta
    50221 ccccttaccc tggggctgat ttttttgctg gctatcatgg aaaccattta cgtggtcacc
    50281 ggcaaaacaa tctaccgcga tatgacgcgc ttctggggta agctcttcgg tatcaacttt
    50341 gctcttggcg tggctaccgg cctgaccatg gagtttcagt ttggtactaa ctggtcattc
    50401 tattccaact atgtgggcga tatttttggc gcaccgctgg cgatggaagc attaatggcc
    50461 ttcttcctcg aatccacctt tgtcgggctg ttcttcttcg gctggcagcg gctgaataaa
    50521 taccagcacc tgctggtgac gtggctggtg gcgttcggtt caaatctctc tgcattgtgg
    50581 atattgaatg ccaacggttg gatgcaatac ccgaccggtg cgcattttga tatcgacacc
    50641 cttcgtatgg aaatgaccag cttcagcgag ctggtcttta acccggtcag ccaggtgaaa
    50701 tttgtgcaca ccgtaatggc gggctacgtg actggggcca tgtttattat ggcgatcagc
    50761 gcctggtatt tactgcgcgg acgggagcgc gatgtcgcat tacgctcgtt tgccatcggt
    50821 tccgtcttcg gtactctggc gattatcggc acgctgcaac tcggagacag ttctgcgtat
    50881 gaagtcgcgc aagtacaacc ggtaaaactg gcggcgatgg aaggggaatg gcaaacggaa
    50941 cctgcacctg caccgttcca tgtggttgcc tggccggaac aggatcaaga gcgtaacgcc
    51001 tttgctatca aaattcccgc gctgctaggg atcctcgcca ctcactcatt agataaaccc
    51061 gtgccgggtc tgaagaattt gatggctgaa acctacccac gcttgcaacg cggacgtatg
    51121 gcctggctgt taatgcagga aatatcgcaa ggcaatcgtg agccgcatgt gttgcaggca
    51181 tttcgggagc tggaaggcga ccttggttac ggcatgttgc tctcccgcta tgcgccggat
    51241 atgaatcatg tcacagccgc acagtaccag gcggcgatgc gtggcgcgat acctcaggtt
    51301 gcgccggtat tctggagttt ccgcatcatg gtgggctgtg gttccctgct gctactggtg
    51361 atgcttattg cgcttgtcca gacgctgcgt ggcaaaatcg accagcatcg ctgggtgctg
    51421 aaaatggcgc tctggagttt gccgctgccg tggattgcga ttgaagccgg gtggtttatg
    51481 accgagtttg gtcgtcagcc gtgggcgata caggacatct taccgacata ctccgcgcac
    51541 tccgccttaa ccacaggaca actggctttc tcactgatca tgatcgtagg gctttacacc
    51601 ctgttcttaa tcgccgaagt ctacctgatg cagaaatatg cccgtctggg gccgagcgcg
    51661 atgcagagtg aacaaccgac gcagcaacag gggtaaagga gaaaatcatg tttgattatg
    51721 aaacattgcg cttcatctgg tggctgctga ttggcgtgat cctggtagtc tttatgatct
    51781 ccgacggatt tgacatgggg atcggctgtc tgctgccgct ggtggcgcgt gatgatgatg
    51841 aacgccggat agtgattaat agcgttggtg cgcactggga aggcaatcag gtctggttga
    51901 tcctcgcagg tggggcatta tttgccgcct ggcccagagt gtatgcagcg gcgttttccg
    51961 gcttttatgt ggcgatgatc ctggtgctgt gctcgctgtt cttccgcccg ctggcctttg
    52021 attatcgcgg aaaaattgcc gatgcacgct ggcgtaaaat gtgggatgcc ggtctggtta
    52081 tcggcagtct ggtgccgccg gtagtcttcg gtatcgcctt cggcaacttg ttgctaggcg
    52141 tgccgtttgc ctttacaccg caattacgcg tggagtatct cggcagcttc tggcaactgc
    52201 tgacgccatt ccctttattg tgcggattgc tcagccttgg gatggtgatt ttgcaaggtg
    52261 gcgtctggtt acaactgaaa actgttggtg tgattcatct gcgttcacag ctggcgacca
    52321 aacgcgccgc actgctggtg atgctgtgct ttttgctggc gggttactgg ctgtgggtcg
    52381 gtattgatgg ctttgtactg ctcgcccagg atgctaacgg tccttccaat ccgttaatga
    52441 aactggtggc agtgctacct ggtgcctgga tgaataattt tgtcgagtcg cccgttttgt
    52501 ggatcttccc gctgctgggg ttcttctgcc cattgctgac ggtgatggcg atttatcgtg
    52561 gtcgcccggg ttggggattt ttgatggcgt cattgatgca atttggcgtg attttcacgg
    52621 caggcatcac gctgttcccc tttgtcatgc cgtcaagcgt gagtccgatc tccagcctga
    52681 cgttgtggga cagtacttcc agccagctga cgctgagcat tatgttggta atcgtgctga
    52741 tatttttgcc cattgtgttg ctctacactc tctggagcta ctacaaaatg tgggggcgca
    52801 tgacgacaga aactctccgc cgtaacgaaa acaagttgta ctaaggagta ggaacaatgt
    52861 ggtatttact ttggttcgtc ggcattttgt tgatgtgttc gctctccacc cttgtgttgg
    52921 tatggttgga cccgcgtctg aaaagttaac gaacgtaagc ctgatgaggc gcattagcgt
    52981 cgcatcaggc aatcaataat gtcggatatg aaaagcggaa acatatcgat gaaagcgatc
    53041 ttgatcccat ttttatctct tctgattccg ttaaccccgc aatctgcatt cgctcatagt
    53101 gagccggagc tgaagctgga aagtgtggtg attgtcagtc gtcatggtgt gcgtgcacca
    53161 accaaggcca cgcaactgat gcaggatgtc accccagacg catggccaac ctggccggta
    53221 aaactgggtt ggctgacacc gcgcggtggt gagctgatcg cctatctcgg acattaccaa
    53281 cgccagcgtc tggtagccga cggattgctg acgaaaaagg gctgcccgca gcctggtcag
    53341 gtcgcgatta ttgctgatgt cgacgagcgt acccgtaaaa caggcgaagc cttcgccgcc
    53401 gggctggcac ctgactgtgc aataaccgta catacccagg cagatacgtc cagtcccgat
    53461 ccgttattta atcctctaaa aactggcgtt tgccaactgg ataacgcgaa tgtgactgac
    53521 gcgatcctca gcagggcagg agggtcaatt gctgacttta ccgggcatcg gcaaacggcg
    53581 tttcgtgaac tggaacgggt gcttaatttc ccgcaatcaa acttgtgcct taaccgtgag
    53641 aaacaggacg aaagctgttc attaacgcag gcattaccat cggaactcaa ggtgagcgcc
    53701 gacaatgtct cattaaccgg tgcggtaagc ctcgcatcaa tgctgacgga gatatttctc
    53761 ctgcaacagg cacaaggaat gccggagccg gggtggggaa ggatcactga ttcacaccag
    53821 tggaacacct tgctaagttt gcataacgcg caattttatt tactacaacg cacgccagag
    53881 gttgcccgca gtcgcgccac cccgttattg gatttgatca tggcagcgtt gacgccccat
    53941 ccaccgcaaa aacaggcgta tggtgtgaca ttacccactt cagtgctgtt tattgccgga
    54001 cacgatacta atctggcaaa tctcggcggc gcactggagc tcaactggac acttccaggt
    54061 cagccggata acacgccgcc agggggtgaa ctggtgtttg aacgctggcg tcggctcagc
    54121 gataacagcc agtggattca ggtttcgctg gtcttccaga ctttacagca gatgcgtgat
    54181 aaaacgccgc tgtcattaaa tacgccgccc ggagaggtga aactgaccct ggcaggatgt
    54241 gaagagcgaa atgcgcaggg catgtgttcg ttggccggtt ttacgcaaat cgtgaatgaa
    54301 gcacgcatac cggcgtgcgc tctccatcaa gacaaataaa catttgccgc tgttggtcct
    54361 cgcaggccat ttacgcggca aaattccacg cgtaatcctg gtataagcac ttctgcgtcg
    54421 cggggagtga atgcggaaat atggacctgg acttctttac gaccgtcgga ggggataatg
    54481 aatcctttgc cgcttttgcg atcaaaggtt ttgacaattc ctgtcatttt acgggacaaa
    54541 caaattcctt actgaaaata ctgcgctgca ctatacgggg ttaataaaat aaagccagcg
    54601 atatttaaga ccgccggacg gctaaaataa aatttgctta atctcaatta tcatgcgtta
    54661 atagctgcgt cggtttgaaa gacagacagc atacaaagta gtttactaaa gcagttctca
    54721 ttatcaggca ttatcccctt cttttgagtc tctctcctga acactaagta gtttctgtat
    54781 taaagccctg tttgccgaaa ggcccaaaat gaaggaagta aaatatgtct aataaaatga
    54841 ctggtttagt aaaatggttt aacgcagata aaggttttgg ctttatcact cctgatgatg
    54901 gcagcaaaga cgttttcgtc catttcaccg ccattcagag caatgaattc cgcacgctga
    54961 acgaaaatca gaaagttgaa ttttctattg agcaggggca acgtggtccc gcggcagcga
    55021 acgttgttac gctctaaggt tgccgttatt actcaacatc tccatttccg ctgtccatgt
    55081 tgtcatggtt cacagtaccg cacatcggca ttcgatgtga cggagcgaaa tcctttagga
    55141 gcaaagtgta ttttttgtaa atcgacgatg atcacctttg ataacgtcgc gctgcaaata
    55201 cgcactgacc atgcgccgct ggatttcaca aaataatatc aggctccctt gtggagcctt
    55261 ttttatatct gccttatttt tcttcaacgc tgtatgtata gtaagcgata acctgttgat
    55321 tattgaatct ttcggggaga tggcttataa catttcttac ctgaccaggg taccgggaac
    55381 caacacctta ctggcgtgtt gctgtctttt aagaccagaa gaggttaaca gtgaatattg
    55441 aagagttaaa aaaacaagcc gaaacggaaa tcgccgactt tatcgcacaa aaaatcgccg
    55501 agctgaacaa aaatacaggg aaagaagtct ctgaaattcg cttcaccgca cgagaaaaaa
    55561 tgaccgggct tgaaagttat gatgtcaaaa taaaaataat gtgattttgt gaacatcacc
    55621 cgtgcgaggt gatgttccgc ttgttgctaa tttagtgacc aatcattggc gcttgtggaa
    55681 ttaagcgtcg gtacaattcc tccggtaccg ggctttgcca tactcccgca tacattgcgt
    55741 aaccaatcac cgcaaacata atccccagaa tcagtagcgt cattaaccag ccagacaacg
    55801 caaaggcttt tttatttgcc gcaggttttt gcagtgaaaa ggtcaatgtt gaggctaccg
    55861 gacatgactc tacgcaagtc atacagccgg tacattccac tgttcgtacc tgaattaatt
    55921 tatcgaccgg gatccgtgat gggcaatttt ttgcgcattt gccacagtcg atacaacttt
    55981 cggcattgcg acgaatctta aacggcgata atagcgaaac cacgcccatc agcgcgccat
    56041 atgggcaaag ataacgacac caggcatggc gaataaacag gctggcaatc agcaaaacgg
    56101 tcacgctgat taatatcgcg gtccccatat gacgaaagaa atcgagcatt ttaacgtcca
    56161 tcaccacgct gtagggcgac aacataaaat agtgaatcgc ctgagcgggc atcaataacg
    56221 caatatagag aaaaaaactc aacagcaaat acttcacgcc gcgcagagga atatccagcc
    56281 agcggggaag gacacattgc cgaccaaaca gtttgttacc gagatcgccg attaattcag
    56341 aaagcgtacc aaccgggcat aaccatgaac aaaaggcctt tttgagtaat agactgatga
    56401 cgataaaagc gaccaataac agcattgcgg cggcgtggac ggacggtaac tgacctgttg
    56461 caaggctata tttcagattc atcagaccgg caatcggtag ccagccttcg atacctcccg
    56521 gtctggcgac aaatgtcgta ctacctgccg tttcgtaata gcgcacccaa taccagaacg
    56581 tgatggcaat ataaatattc attgccaaca gcaataattg cgtcgcttta cgccaggtcg
    56641 tggcattgcg ccagttattc cacggtaatt tgccgcccgt cgtgcctggc cgccgctgcc
    56701 agcgggttct ttttttctct gccatgattt tgccagtccg ttaagttgca taccaaatgc
    56761 cactattcta gttattctta actggctgat attgattcaa atcgcgttca ggtctttctt
    56821 atgcaaccat gcttccagag cggcaacact gcgtgtaatt tcttcgtgtg gaaggggggc
    56881 agataatggc tgctgctcca gttgtgcgca tagctggctg gcgctacgca ttcccaggct
    56941 cgagcaactg ctttttagct gatgtgcggc acgctttatt ttctcgctat cctgactggc
    57001 gcgggcaatg tcgatttcat cgagaagcgg cagggcatgt tgtgtaaata ataccagcca
    57061 ttcgtggatc ttctccgtct ccattaactg agcatcttca ttgagttgcg atacatccag
    57121 cggttgatcg ttattgactt gcagttggag atagtgcgcc agtaactgac cgagcacttc
    57181 acgcggcacc ggtttaggga taattccgcg aaataatgaa ctggtacgct ggcgcaacgt
    57241 ttcgtcgatg acatgagcgc taaagccaat caaaaccagc gacggatatt gctgtgccag
    57301 ttgccgggca agggtaatac cgtcgatatc cggcagatca aaatccacca gtgcggcagc
    57361 aaacggttcg ctattttgca acgtctctat cgcgtgcgcg gcattgccaa cagcaacgac
    57421 ctgcgcccca ctgctgttca acatttcgac ggtaattcgt tgggttagcg ggttatcttc
    57481 aatcaacagt aatcgtaaac cgtccagacg caccgcctga ttgactgttt ttggcacggg
    57541 agcagtggca acacgtaacg gcaagcgtaa acaaaaacag cttccaactt ccggcgtgct
    57601 ggtggcgctc agttcgccgc ccatcgcctg ggccagacgg ctactgatgg tcagccccag
    57661 cccggtgccg ccgcgtttgc cgctaacctg aacaaatggc tggaagattt cagccagttt
    57721 cgtgggatca ataccgcagc cgctgtcttc cacttcgacc agccattgtt cgccatcagt
    57781 gcgactacgc aggataatgt gcccttcgtc ggtgaaacgc agggcgttgc tcaacaggtt
    57841 ggtgatgacc tgacgaatac gccgcggatc gcccattaac gcagtcggta catcgtcggc
    57901 aatttctgtt gccaggcgaa tcgggcgacc tttcaccctt ccgctcatta attgcagggt
    57961 actttccagc agcgggcgcg gttcaaaggg ttcatcgctg accgaaacat tcttgccgcc
    58021 tgcttcgata gccgaataat cgagaatatc gttgaggatg gtcagcaacg attcaccaga
    58081 gtcagtaatt gcgcgcaaat catcgcgctg ggcgttaagt gcggggttat ctgccagcag
    58141 ctgtgcggtg ccgagaatac cgtacagcgg tgtgcggatc tcatgactca tcgccgccag
    58201 aaacgccgat tttgcctggc tggctttttc tgcttccgcc cgtgcctgcc ggtgttctat
    58261 caccatttcc tgcaattcag ctgtacgcgc tttgacctgc gccgccagct gttcgcggtg
    58321 gcgattcagt gcatgaacac tgctgcgaaa cgcatccatc agccgcccga tggtatccag
    58381 ctcccgcacg ccagcggttt ccgggaaagg ggagtcaata tcaccgtcca gcagccgttg
    58441 cagcgcctgc gtttgttcgg caagtggacg cgtgactgag cgataaacca cgcgccagag
    58501 gatcagaatc agtgcgcaaa gtgaaaccat ccccagcaat aacaggctgt attgcccgcg
    58561 tgcactggct ttttccagat gcgccagtcc gtgctgatta cgcagctcaa tggtgtcgac
    58621 cagctgactg acttcgctac taaactgcgc gaactgggcg atgttatttt gggcgagagt
    58681 ttgcaggtga ttgctgattt cactgtcctg ctgatacagc gccagcaaat cgctatattg
    58741 gctaacggaa gttaacgttg ttgcgacctg agcacgaact cccggatctt caatgcgtat
    58801 ttgccgacgt tgcagaattt tcaccgcatt attgagctgc ttttccagcg ttggcgcatt
    58861 tttctggatc tgttccaacc ccagattcat caccatttgc tgcacccgca gggcgctaag
    58921 gcgcagttca ttcatctggt taacatactc aagatcgata tcaatcagcc gatcgagtgc
    58981 actttctgcg gcctgacgct gatgttgttc gatcaaatcg taaatcccgg cctgggtcgc
    59041 tccggcggag gttgccgcat tattcgcctg accttgcgcc aggcgtgcga tttcatcggc
    59101 ggcggcgact atctgctgac tgagttgctg ttgttgctgg cgcagttgca aacgctgtcc
    59161 caccagttcc ccttgctgac gtaacgaacg ggagatctcc tgctcctgta gttcaatagc
    59221 ggtggtgtca aaatcttgtt cccgtaacgc ttgcagcaac gcattaatct tcaggctttg
    59281 tgcggtgagc attcgcccct gcgcctgcca catcttttcg ttatcggcac tggtcaggtt
    59341 ctgcgcggcg aaaagttccc aggcgctggc ttcgctcaac tggcgcgcca tattcatggt
    59401 aggaatcaat gcctgagtgt tgtctttttc cacctggctg ataaagcgca ggttgtacca
    59461 tcccaccagg gtactggtca gggttaacag cgccatcagg gcaaagccca tccagagtct
    59521 tcgggtcagg gttaaattca cggtcggtgc acttaaggtg aaaaaggttg agtcgcaaag
    59581 cggaatgcat ctagcataaa gccttattat tgatgaggct atcatgcgcg tactgctatt
    59641 tttacttctt tcccttttca tgttatcggc attttcggct gataacctgt tgcgctggca
    59701 tgatgcgcag catttcacgg tgcaagcctc tacgccgctt aaagccaaac gcgcatggaa
    59761 actgtgcgcg ctttatccca gcctgaaaga ttcatactgg ttatcgttga actatggtat
    59821 gcaggaggct gctcgccgct acggtgtgga tttaaaagtg ctggaggcag gcggctacag
    59881 ccagttggct acccagcaag cacaaatcga ccagtgtaaa cagtggggcg cagaggccat
    59941 tttgctcggt agtagcacga cgtcatttcc cgatctgcaa aagcaggtag caaatctgcc
    60001 ggtgatcgaa ctggtaaatg ctattgatgc tccccacgtg aaaagccgcg ttggtgtgcc
    60061 ctggtttcag atgggctatc aaccagggcg atatctggtg caatgggcgc acggtaaacc
    60121 actgaacgtg ctgttgatgc ccggacccga taacgccggg ggcagtaagg agatggtcga
    60181 gggttttcgc gcagccattg ccggaagccc ggtgcgtatt gttgatattg cgcttggtga
    60241 taacgatatt gaaatccagc gtaacctgtt gcaggagatg ctggagcgcc atccagaaat
    60301 cgacgtcgtt gccggaacgg ccattgcggc agaggcggca atgggggaag ggcgtaacct
    60361 gaaaacgccg cttaccgtgg tatcgtttta tctgtcgcat caggtatacc gaggcctgaa
    60421 gcgagggaga gtcattatgg cagccagcga tcaaatggtc tggcaggggg aactggcggt
    60481 tgagcaggcc atcaggcaat tacaggggca atcggtgtct gataatgtca gcccaccgat
    60541 tttagttctg acgccgaaaa atgccgaccg tgaacatatc cgccgctcgc tgtcaccagg
    60601 gggatttcgt ccggtctatt tttatcagca cacatcagcg gctaagaaat aaccttcgcc
    60661 atgttgcgtc accagtaaat ccgcgctgag tttatggcgt aaacgacgaa ttaacacatc
    60721 gacggtgcgc aggtcagggt tttccacccg acgcgcagaa agcatacgca gcagacgttc
    60781 acggctaaga atttcgcccg gattcgtcac aaatgccacc aacatttcat actctgcgcg
    60841 ggtcagttta attggctcgc catctcgctc cagcgtatgg cgcgacacat tcaggcaata
    60901 accggcaaag cgatagcagt tgtcctgagt gtgcggttga gcttgtcgcg cgaggtcgat
    60961 tcgccagagc agatttttca cccgtactac cagttcgcgc agttccagcg gtttggtgac
    61021 ataatcgtct gcacccattt ccagcccaac aatacggtca atccgatcgc tgcgtccggt
    61081 aaccagaata atccccaccg ttgagcgttc tcgcagggcg cgggttaaca tcaggccatt
    61141 ttcatcgggc aagttgatat ccagcagaat taaatccacc ggctgattct gcataatttc
    61201 ccgcagcccg gcaccgctcg ccgtaacgga aacggtatac ccctcctgag tgaagtaaga
    61261 ttgtaatcgc gcctgggtaa ccggttcatc ttcaacaata acaatgtgat gtggcatcag
    61321 aggattttac tcattctgtt catatctgtt catattctgc cgtaagccgt tcatcctgac
    61381 cagtgccgct gttcatattt actcattaag atcgcttcac taaaccataa ttttacaggg
    61441 gctattatgc ggaaattctg gaacgcgcta cgccgaccca gtgctcgttg gtcggtactg
    61501 gcgctggtcg ctattgggat tgtgattggc attgcgctga ttgtattgcc acacgttggg
    61561 atcaaagtca ccagcacaac cgaattttgt gtcagttgcc acagtatgca accggtgtat
    61621 gaagaatata aacagtcggt gcatttccag aacgcctccg gcgtgcgagc tgaatgccat
    61681 gactgtcata tcccgccgga tattccaggc atggtgaagc gcaaactgga agcgagcaac
    61741 gatatctacc agacctttat tgcccactcc attgatacac ctgagaaatt tgaagccaaa
    61801 cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag aaaacaactc ggcaacctgc
    61861 cgctcctgcc ataactacga tgcgatggat catgccaagc agcatcctga agcggcacgt
    61921 cagatgaagg tggcagcgaa agataatcaa tcctgcatcg actgtcataa aggtattgcc
    61981 caccagttgc cggatatgag tagtggcttc cgtaagcagt tcgatgagct gcgcgccagt
    62041 gctaatgaca gtggtgacac gctgtactcc attgatatta agccgattta tgcggcgaaa
    62101 ggcgataaag aagcctctgg ttctctgctg cctgcttcgg aagtgaaagt ccttaaacgt
    62161 gacggtgact ggctgcaaat tgaaattacc ggctggacgg aaagcgccgg acgtcagcgt
    62221 gtactcaccc agttccctgg caaacgcatc tttgttgcct cgattcgtgg tgatgtgcag
    62281 cagcaggtga aaacgctgga gaaaaccacc gttgccgaca ctgataccga gtggagcaag
    62341 ttgcaggcca ctgcgtggat gaagaaaggt gacatggtga acgatatcaa accgatctgg
    62401 gcttatgctg attcgttgta caacggcacc tgtaaccagt gccacggcgc accggaaatc
    62461 tctcactttg acgctaacgg ttggatcggc acgctcaacg gcatgattgg ctttaccagc
    62521 ctcgataaac gtgaagaacg caccttgttg aaatatctgc aaatgaatgc gtctgacacc
    62581 gcaggtaagg ctcacggcga taagaaggaa gaaaaataat gaacaataac gatctctttc
    62641 aggcatcacg tcggcgtttt ctggcacaac tcggcggctt aaccgtcgcc gggatgctgg
    62701 ggccgtcatt gttaacgccg cgcagtgcga ctgcggcgca agcggcgact gaggctgtca
    62761 cctcgaaaga gggcattctt accgggtcgc actggggggc tatccgcgcg acggtgaagg
    62821 agggtcgctt tgtgacggca aaaccgttcg aactggataa atatccgtcg aaaatgatcg
    62881 ccggattgcc ggaccacgta cacaacgcgg cgcgtattcg ttacccgatg gtacgtgtgg
    62941 actggctgcg taagcgccat ctgagcgaca cctcccagcg cggtgataac cgttttgtgc
    63001 gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga actggaacgt gtgcagaaaa
    63061 cgcacgggcc gagtgccttg ctgaccgcca gtggttggca atcgacgggg atgttccata
    63121 acgcttcggg gatgctggcg aaagcgattg ccttgcatgg taatagcgtg ggtacaggcg
    63181 gagattactc taccggtgct gcgcaggtga tcctgccgcg cgtggttggc tcaatggaag
    63241 tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa cagcaaaacc attgtgctgt
    63301 ggggctccga tttgctgaaa aaccagcaag cgaactggtg gtgcccggat cacgatgttt
    63361 atgaatatta cgagcagcta aaagcgaaag tcgccgccgg tgaaattgag gtcatcagca
    63421 tcgatccggt tgtcacatcc acccatgagt atctggggcg cgagcatgtg aagcacattg
    63481 cggttaaccc gcaaactgac gtgccgctgc aactggcgct ggcgcatacg ctgtacagtg
    63541 aaaacctgta cgacaaaaac ttcctcgcta actactgtgt aggttttgag gagttcctgc
    63601 cgtatctgct gggtgagaaa gacggtcagc cgaaagatgc cgcatgggct gaaaaactga
    63661 ccggcattga tgccgaaacc attcgtgggc tggcgcggca gatggcggcg aacagaacgc
    63721 agattattgc tggctggtgc gttcagcgta tgcagcacgg tgaacagtgg gcgtggatga
    63781 ttgtcgttct ggcggcgatg ctggggcaaa ttggcctgcc aggtggtggc tttggttttg
    63841 gctggcacta taacggcgca ggcacgccgg ggcgtaaagg cgttattctg agtggtttct
    63901 ccggctctac gtcgattccg cctgttcacg acaacagtga ctacaaaggt tacagcagca
    63961 ccattccgat tgcccgtttt atcgatgcga tcctcgaacc ggggaaagtg atcaactgga
    64021 acggtaaatc ggtaaaactg ccgccgctga aaatgtgtat ttttgccgga actaacccgt
    64081 tccatcgcca tcagcagatc aaccgcatta ttgaaggctg gcgcaagctg gaaacggtta
    64141 tcgccataga taaccagtgg acctcaacct gccgctttgc cgatatcgta ctgcctgcga
    64201 ccacgcagtt tgagcgtaac gatctcgacc agtacggcaa ccactccaac cgtggcatta
    64261 tcgccatgaa acaggtcgtg ccgccgcagt tcgaggcgcg caacgacttc gatattttcc
    64321 gcgagctgtg ccgtcgcttt aatcgcgaag aagcctttac cgaagggctg gacgaaatgg
    64381 gctggctgaa acgcatctgg caggaaggtg tacaacaggg caaaggacgc ggcgttcatc
    64441 tgccagcgtt tgatgacttc tggaataaca aagagtatgt cgagtttgac catccgcaga
    64501 tgtttgttcg tcaccaggca ttccgcgaag atccggatct cgaaccgctg ggtacgccga
    64561 gtggcctgat tgagatctac tcgaaaacca tcgccgatat gaactacgac gattgtcagg
    64621 ggcatccgat gtggtttgag aaaatcgaac gctcccacgg cgggcccggc tcgcagaagt
    64681 atccgttgca tctgcaatct gtgcatccgg atttccgact tcactcgcag ttatgtgagt
    64741 cggaaacgct gcgtcagcaa tatacggtag cgggcaaaga gccagtgttc attaacccgc
    64801 aggatgccag cgcgcgtggt attcgtaacg gtgatgtggt acgcgtcttt aacgctcgtg
    64861 gccaggtgtt ggctggagca gtggtttctg accgctatgc acccggcgtg gcacgaattc
    64921 atgaaggggc atggtacgat ccagataaag gcggcgagcc tggtgcgctg tgcaaatacg
    64981 gtaaccccaa cgtgctgacc atcgacatcg gtacatcgca gctggcgcag gcgaccagtg
    65041 cgcacactac gctggtggaa attgagaagt acaacggaac agtggagcaa gtgacggcgt
    65101 ttaacggccc cgtggagatg gtggcgcagt gcgaatatgt tcccgcgtcg caggtgaaat
    65161 catgaccacg ctgacagcac aacagattgc ctgtgtttac gcctggctgg cgcagttgtt
    65221 ctcccgtgaa ctggacgatg aacaactgac gcaaatcgcc agtgcgcaga tggctgaatg
    65281 gttttcgttg ctgaaaagtg aaccgccgct cactgcggcg gtgaatgagc tggaaaaccg
    65341 tattgccgtt ctgacagtac gtgacgacgc ccgtctggaa ctggccgctg acttttgcgg
    65401 cctgtttctg atgaccgaca aacaagcggc gctgccttat gcatcggcct acaaacagga
    65461 cgagcaagag attaaacgct tgttagttga ggcagggatg gaaacctgcg gcaatttcaa
    65521 cgaaccggca gatcatctgg cgatctatct cgagttgttg agtcatctgc atttttcgct
    65581 gggagagggg accgttcctg cgcgaagaat cgacaatttg cggcaaaaaa cactgacggc
    65641 gctgtggcaa tggttatcag agtttgctgt gcgttgtcgt cagtatgaca gctttggttt
    65701 ttacgcggca ctaagccagt tattgctggt gttagtggag tgcgaccacc aaaacagata
    65761 acgtcgtttg ttggcctgat aagacgcgtt
//
LOCUS       DAPOZO010000015        63742 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPOZO010000015 DAPOZO010000000
VERSION     DAPOZO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.015"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            25..1314
                     /gene="uraA"
                     /locus_tag="ROI21_001014"
     CDS             25..1314
                     /gene="uraA"
                     /locus_tag="ROI21_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDX5109967.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKMISVLRPEEVVLDAEDADITDK"
     gene            1364..2110
                     /gene="hda"
                     /locus_tag="ROI21_001015"
     CDS             1364..2110
                     /gene="hda"
                     /locus_tag="ROI21_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDX5109968.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(2216..2575)
                     /gene="arsC"
                     /locus_tag="ROI21_001016"
     CDS             complement(2216..2575)
                     /gene="arsC"
                     /locus_tag="ROI21_001016"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDX5109969.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2596..4059)
                     /gene="bepA"
                     /locus_tag="ROI21_001017"
     CDS             complement(2596..4059)
                     /gene="bepA"
                     /locus_tag="ROI21_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDX5109970.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4272..5333
                     /locus_tag="ROI21_001018"
     CDS             4272..5333
                     /locus_tag="ROI21_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDX5109971.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVIGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(5370..6218)
                     /locus_tag="ROI21_001019"
     CDS             complement(5370..6218)
                     /locus_tag="ROI21_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HDX5109972.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLTVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWVSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADHFPVLTISHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(6240..8252)
                     /gene="hyfR"
                     /locus_tag="ROI21_001020"
     CDS             complement(6240..8252)
                     /gene="hyfR"
                     /locus_tag="ROI21_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDX5109973.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQSEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRCMPGESVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(8282..8695)
                     /gene="hyfJ"
                     /locus_tag="ROI21_001021"
     CDS             complement(8282..8695)
                     /gene="hyfJ"
                     /locus_tag="ROI21_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDX5109974.1"
                     /translation="MAEECREIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLSVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDAMSAWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(8688..9446)
                     /gene="hyfI"
                     /locus_tag="ROI21_001022"
     CDS             complement(8688..9446)
                     /gene="hyfI"
                     /locus_tag="ROI21_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HDX5109975.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKIKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAVEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLREADDPRLNSIVQQLFRILRGLHG"
     gene            complement(9443..9988)
                     /gene="hyfH"
                     /locus_tag="ROI21_001023"
     CDS             complement(9443..9988)
                     /gene="hyfH"
                     /locus_tag="ROI21_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDX5109976.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNTRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDEPLVAKEQL"
     gene            complement(9998..11713)
                     /gene="hyfG"
                     /locus_tag="ROI21_001024"
     CDS             complement(9998..11713)
                     /gene="hyfG"
                     /locus_tag="ROI21_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDX5109977.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(11703..13283)
                     /gene="hyfF"
                     /locus_tag="ROI21_001025"
     CDS             complement(11703..13283)
                     /gene="hyfF"
                     /locus_tag="ROI21_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HDX5109978.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATLAVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLLIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGELGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(13288..13938)
                     /gene="hyfE"
                     /locus_tag="ROI21_001026"
     CDS             complement(13288..13938)
                     /gene="hyfE"
                     /locus_tag="ROI21_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDX5109979.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMTLLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(13950..15389)
                     /gene="hyfD"
                     /locus_tag="ROI21_001027"
     CDS             complement(13950..15389)
                     /gene="hyfD"
                     /locus_tag="ROI21_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HDX5109980.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(15406..16353)
                     /gene="hyfC"
                     /locus_tag="ROI21_001028"
     CDS             complement(15406..16353)
                     /gene="hyfC"
                     /locus_tag="ROI21_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDX5109981.1"
                     /translation="MRQTLCDGYLIIFALAQAVILLALTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRMMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     DSTHIEMISKTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKLGLGLKQIVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLTHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(16364..18382)
                     /gene="hyfB"
                     /locus_tag="ROI21_001029"
     CDS             complement(16364..18382)
                     /gene="hyfB"
                     /locus_tag="ROI21_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HDX5109982.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFNFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLMAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVFIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFCALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(18382..18999)
                     /gene="hyfA"
                     /locus_tag="ROI21_001030"
     CDS             complement(18382..18999)
                     /gene="hyfA"
                     /locus_tag="ROI21_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDX5109983.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQADGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(19252..19722)
                     /gene="bcp"
                     /locus_tag="ROI21_001031"
     CDS             complement(19252..19722)
                     /gene="bcp"
                     /locus_tag="ROI21_001031"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDX5109984.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(19722..20294)
                     /locus_tag="ROI21_001032"
     CDS             complement(19722..20294)
                     /locus_tag="ROI21_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDX5109985.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            20440..21318
                     /gene="dapA"
                     /locus_tag="ROI21_001033"
     CDS             20440..21318
                     /gene="dapA"
                     /locus_tag="ROI21_001033"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDX5109986.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAAHDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            21335..22369
                     /gene="bamC"
                     /locus_tag="ROI21_001034"
     CDS             21335..22369
                     /gene="bamC"
                     /locus_tag="ROI21_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDX5109987.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            22582..23295
                     /gene="purC"
                     /locus_tag="ROI21_001035"
     CDS             22582..23295
                     /gene="purC"
                     /locus_tag="ROI21_001035"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDX5109988.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            23463..24326
                     /locus_tag="ROI21_001036"
     CDS             23463..24326
                     /locus_tag="ROI21_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDX5109989.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            24341..26356
                     /gene="tmcA"
                     /locus_tag="ROI21_001037"
     CDS             24341..26356
                     /gene="tmcA"
                     /locus_tag="ROI21_001037"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDX5109990.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPVAENHCFPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTANNDAILWRQNQPFTL
                     VHFAPRTDWHPATGTPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHEPQGDIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEGEHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALYALDDVRTERLRDRITQWQFFH"
     gene            26430..27128
                     /gene="ypfH"
                     /locus_tag="ROI21_001038"
     CDS             26430..27128
                     /gene="ypfH"
                     /locus_tag="ROI21_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDX5109991.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQERVDAIMSTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(27209..27409)
                     /locus_tag="ROI21_001039"
     CDS             complement(27209..27409)
                     /locus_tag="ROI21_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDX5109992.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(27437..28564)
                     /gene="dapE"
                     /locus_tag="ROI21_001040"
     CDS             complement(27437..28564)
                     /gene="dapE"
                     /locus_tag="ROI21_001040"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDX5109993.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(28568..28924)
                     /locus_tag="ROI21_001041"
     CDS             complement(28568..28924)
                     /locus_tag="ROI21_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDX5109994.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKIIDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQHFFHEV"
     gene            29305..29364
                     /gene="ypfM"
                     /locus_tag="ROI21_001042"
     CDS             29305..29364
                     /gene="ypfM"
                     /locus_tag="ROI21_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDX5109995.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(29463..32576)
                     /gene="acrD"
                     /locus_tag="ROI21_001043"
     CDS             complement(29463..32576)
                     /gene="acrD"
                     /locus_tag="ROI21_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDX5109996.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMTARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(32740..34440)
                     /gene="narQ"
                     /locus_tag="ROI21_001044"
     CDS             complement(32740..34440)
                     /gene="narQ"
                     /locus_tag="ROI21_001044"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDX5109997.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            34646..36625
                     /gene="aegA"
                     /locus_tag="ROI21_001045"
     CDS             34646..36625
                     /gene="aegA"
                     /locus_tag="ROI21_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDX5109998.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRVD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVMVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            36693..37268
                     /gene="nudK"
                     /locus_tag="ROI21_001046"
     CDS             36693..37268
                     /gene="nudK"
                     /locus_tag="ROI21_001046"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDX5109999.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     NKALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            37394..38437
                     /locus_tag="ROI21_001047"
     CDS             37394..38437
                     /locus_tag="ROI21_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDX5110000.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38540..40543)
                     /gene="tkt"
                     /locus_tag="ROI21_001048"
     CDS             complement(38540..40543)
                     /gene="tkt"
                     /locus_tag="ROI21_001048"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDX5110001.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAARTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKKAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(40563..41513)
                     /gene="tal"
                     /locus_tag="ROI21_001049"
     CDS             complement(40563..41513)
                     /gene="tal"
                     /locus_tag="ROI21_001049"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDX5110002.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGRKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            41802..44081
                     /gene="maeB"
                     /locus_tag="ROI21_001050"
     CDS             41802..44081
                     /gene="maeB"
                     /locus_tag="ROI21_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDX5110003.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRSDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            44374..44709
                     /gene="eutS"
                     /locus_tag="ROI21_001051"
     CDS             44374..44709
                     /gene="eutS"
                     /locus_tag="ROI21_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDX5110004.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            44722..45201
                     /gene="eutP"
                     /locus_tag="ROI21_001052"
     CDS             44722..45201
                     /gene="eutP"
                     /locus_tag="ROI21_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDX5110005.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGDYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            45176..45877
                     /gene="eutQ"
                     /locus_tag="ROI21_001053"
     CDS             45176..45877
                     /gene="eutQ"
                     /locus_tag="ROI21_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDX5110006.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESTPVTASVPACVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            45874..46677
                     /gene="eutT"
                     /locus_tag="ROI21_001054"
     CDS             45874..46677
                     /gene="eutT"
                     /locus_tag="ROI21_001054"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDX5110007.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHRQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLAC
                     QAIVGLSDEDLHRLSHQPLRYLGHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            46674..47690
                     /gene="pta"
                     /locus_tag="ROI21_001055"
     CDS             46674..47690
                     /gene="pta"
                     /locus_tag="ROI21_001055"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDX5110008.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFALRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGTADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            47729..48022
                     /gene="eutM"
                     /locus_tag="ROI21_001056"
     CDS             47729..48022
                     /gene="eutM"
                     /locus_tag="ROI21_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDX5110009.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            48129..48416
                     /gene="eutN"
                     /locus_tag="ROI21_001057"
     CDS             48129..48416
                     /gene="eutN"
                     /locus_tag="ROI21_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDX5110010.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            48428..49831
                     /locus_tag="ROI21_001058"
     CDS             48428..49831
                     /locus_tag="ROI21_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDX5110011.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            49842..50678
                     /gene="eutJ"
                     /locus_tag="ROI21_001059"
     CDS             49842..50678
                     /gene="eutJ"
                     /locus_tag="ROI21_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDX5110012.1"
                     /translation="MAHDEQWLTPRLHIAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGIY
                     AK"
     gene            50668..51855
                     /gene="eutG"
                     /locus_tag="ROI21_001060"
     CDS             50668..51855
                     /gene="eutG"
                     /locus_tag="ROI21_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDX5110013.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAMSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDIGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            52025..53251
                     /gene="eutH"
                     /locus_tag="ROI21_001061"
     CDS             52025..53251
                     /gene="eutH"
                     /locus_tag="ROI21_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDX5110014.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATAAKTEAEAQS"
     gene            53248..54651
                     /gene="eutA"
                     /locus_tag="ROI21_001062"
     CDS             53248..54651
                     /gene="eutA"
                     /locus_tag="ROI21_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDX5110015.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPNSDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            54663..56024
                     /gene="eutB"
                     /locus_tag="ROI21_001063"
     CDS             54663..56024
                     /gene="eutB"
                     /locus_tag="ROI21_001063"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDX5110016.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKWAGDPS
                     LFF"
     gene            56045..56932
                     /gene="eutC"
                     /locus_tag="ROI21_001064"
     CDS             56045..56932
                     /gene="eutC"
                     /locus_tag="ROI21_001064"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDX5110017.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCTTPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            56942..57601
                     /gene="eutL"
                     /locus_tag="ROI21_001065"
     CDS             56942..57601
                     /gene="eutL"
                     /locus_tag="ROI21_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDX5110018.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            57614..58114
                     /gene="eutK"
                     /locus_tag="ROI21_001066"
     CDS             57614..58114
                     /gene="eutK"
                     /locus_tag="ROI21_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDX5110019.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVVESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            58160..59212
                     /gene="eutR"
                     /locus_tag="ROI21_001067"
     CDS             58160..59212
                     /gene="eutR"
                     /locus_tag="ROI21_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDX5110020.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(59218..60117)
                     /gene="hemF"
                     /locus_tag="ROI21_001068"
     CDS             complement(59218..60117)
                     /gene="hemF"
                     /locus_tag="ROI21_001068"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDX5110021.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSTVDGTEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEDDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTNAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPEAGSPEAALSEFIKVRDWV"
     gene            complement(60121..60990)
                     /gene="amiA"
                     /locus_tag="ROI21_001069"
     CDS             complement(60121..60990)
                     /gene="amiA"
                     /locus_tag="ROI21_001069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDX5110022.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISFF
                     HWFDNQKAHSRKR"
     gene            61204..61629
                     /locus_tag="ROI21_001070"
     CDS             61204..61629
                     /locus_tag="ROI21_001070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDX5110023.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            61616..62065
                     /locus_tag="ROI21_001071"
     CDS             61616..62065
                     /locus_tag="ROI21_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDX5110024.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            62126..62701
                     /locus_tag="ROI21_001072"
     CDS             62126..62701
                     /locus_tag="ROI21_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDX5110025.1"
                     /translation="MISLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADSSVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            62797..63696
                     /gene="yfeX"
                     /locus_tag="ROI21_001073"
     CDS             62797..63696
                     /gene="yfeX"
                     /locus_tag="ROI21_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDX5110026.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
BASE COUNT        14998 a        16658 c        17582 g        14504 t
ORIGIN      
        1 acacataata cagaggataa tactatgacg cgccgtgcta tcggggtgag tgaaagaccg
       61 ccacttttac agacaatccc gcttagtttg caacatttgt tcgccatgtt tggtgcaacc
      121 gtcctggtgc ccgtcttatt tcatattaac ccggcgactg tactgttatt taacggtata
      181 ggaacactgc tgtatctctt catctgtaaa gggaaaattc cggcttatct tggttccagc
      241 tttgccttta tttcaccggt attgttactg ttgccgttag ggtatgaagt cgcgctgggc
      301 ggctttatta tgtgcggcgt gctgttctgc ctggtttctt ttatcgtgaa gaaagcgggg
      361 accggctggc tggacgtgct gtttccacct gcggcaatgg gcgcaatcgt tgccgtcatc
      421 ggtctggagc tggcgggcgt agctgccggt atggcaggtt tactcccggc tgaagggcaa
      481 acgccagact ccaaaaccat catcatctcc attaccaccc tggcagtcac ggttttaggt
      541 tccgtgctgt ttcgtggttt cctggcaatt atcccgattt taattggcgt gctggtgggt
      601 tacgcgctct ctttcgcaat gggaattgtc gataccacgc cgattattaa tgctcactgg
      661 tttgcgctgc caaccctcta tacgccgcgc ttcgagtggt ttgccattct gactattctg
      721 ccagcggcgt tagtggttat tgccgaacac gtagggcacc tggtggtaac ggctaatatc
      781 gtcaaaaaag atctgctgcg cgatccaggt ctgcaccgtt cgatgtttgc taacggcttg
      841 tcgaccgtga tctccggctt ctttggctcc acgccaaaca ccacctacgg ggaaaacatt
      901 ggcgtgatgg cgatcacccg tgtttacagt acctgggtta tcggcggggc ggcgattttc
      961 gctatcctgc tttcctgcgt cggtaaactg gctgctgcta tccagatgat cccattgccg
     1021 gtgatgggcg gcgtttcgct gctgctttat ggtgtcatcg gtgcttccgg tattcgtgtt
     1081 ttgatcgaat cgaaagtgga ttacaacaaa gcacagaacc tgatcctgac ttccgtgatt
     1141 ttgatcatcg gtgtcagtgg cgcgaaggta aacatcggcg cggcggagct gaaaggtatg
     1201 gcgctggcga ccatcgtcgg tatcggctta agtttgatct tcaaaatgat tagtgtgctg
     1261 cgccctgaag aagtggtgct ggacgcagaa gacgcggata taacagacaa ataatgtacc
     1321 gaccgggcag tgttgctgcc cggttcaaac atcatgggat tctgtggtaa acttctcgcg
     1381 attttgtgaa atcctggttg aggtatctct gaacacaccg gcacagctct ctttgccact
     1441 ttatcttcct gacgacgaaa cctttgcaag tttctggccg ggggataact cctctttact
     1501 ggccgcgctg caaaacgtgc tgcgtcagga acatagcggt tacatctatc tctgggcacg
     1561 cgaaggcgcg gggcgcagcc atctgctgca cgcggcttgc gcggaattgt cgcagcgtgg
     1621 cgatgcggtg ggctatgtcc cgctggataa acgcacctgg tttgttccgg aagtgctcga
     1681 cggtatggag catttgtcgc tggtctgtat cgacaacatt gagtgtattg caggcgatga
     1741 gttgtgggag atggcgattt tcgatctcta caatcgaatt ctggaatcgg gcaaaacacg
     1801 gttgttaatc accggcgatc gtccaccgcg gcagttgaat ctgggattac cggatctcgc
     1861 gtcgcgtctc gactgggggc agatctacaa attgcagcca ctttctgatg aagataagtt
     1921 gcaggcgcta cagttacgcg cgcgtttgcg tggttttgaa ctgccggaag atgtggggcg
     1981 tttcttgttg aagcggctcg acagagagat gcgcacgtta tttatgacgt tggatcagtt
     2041 ggatcgtgcg tcgattaccg cgcaacgtaa gctgaccatt ccgtttgtga aagaaattct
     2101 gaaactgtag acaagacgtt tattgtcgga tgcgcaccca tagtattcgt cggccggata
     2161 agacgcggca agcgtcgcat ccggcaatgt gcttcaacta gcgatggagc acttcttaac
     2221 ccacgatttc cagcacctgc tccggtggac gaccaattcg cgctttgcca ttcgcgacca
     2281 caatcgggcg ctccattagc ttcggattat ccaccattgc ctgaatcagc gcctcttcgc
     2341 taagcgaact gtcagccagg ttcagttctt tataaagatc ctctttctgg cgcatcaatt
     2401 cacgagcgct attcatcccc agcattttca gcaaatcacg cagcgttgcc gcatcggcgg
     2461 gtgtctcaag gtagagcacc acttctggct ccacgccgtt ttctttcagc aggtttagcg
     2521 tttcccggct ctttgaacag cgtgggttat ggtagatttt tacctgtttg gtcattgttc
     2581 ttcctttaat gcgaattaca tcttggtata aggcttaaag cgttcctgca gctggcgcaa
     2641 ctggtcgatg cgcgcatcgt aacgcgcttg ttgcaggctg cctaatttca cctgagaact
     2701 ggcgctactc aacaaggaaa tggcctgatc gagtcgcccg gcgagcgcat aaccttctgc
     2761 tcgcgcagcc agttcctgat cgcggttatt tagcgcggct tccgcctgtg ccagtaaatc
     2821 ccagccgttg ctgtcatctt tattgttaaa agtgtagcga ttcagaatat tggccgcttc
     2881 ttgtggttga ccgccttgca gataagcgtt cgccaggttg agctgcaaca ctggattggt
     2941 gcgcaaatcg cgggcatttt tcaggcgatt gatagcgtca ttggctttgt tttgcccaag
     3001 atcgatatcg gtagccagat cgagatacca tgcgttgcca ggttctgccg ccagtaacgg
     3061 ttgcagcgtt ttacgcgctt cgtcgtattt attggcttcc atcgcctgta aagcacgacc
     3121 atattgcgcc gctcgttgct gacgaacgtt tcctttcgcc cattcatcca gcaaatcact
     3181 ggtgagctgg ttacgtccgg aattatacat ccccagtgtg cgcgctttcg ccagatagaa
     3241 atcttccgac gactgcacca ccatcggacg catctgatta gcacggttgc gggcatctgc
     3301 cagacgactt tccggcaacg ggtgagtcaa taaaatttcc ggcggacgcg aggagtaacg
     3361 cgcctgatcg agcaattttt cgaggaaggt cggcatcgcc tgcggatcga atcccgagcg
     3421 ttgcagcacc tgaataccaa tgcggtccgc ttcctgttca ttttgctggg tgaaactgat
     3481 catcccttgg cgcgttcccg ccagtgtacc ggtcagcgca gccatccccg cctgcggact
     3541 ggccatcgcc agtaaaatag aacctaatgc gccgacccag gtcagcggcg cgctgcgctg
     3601 ctgatcttcc atcgctcgcg ccaggtgacg ttgggtgacg tgggagattt cgtgcgccat
     3661 aactgaagcc agttgacttt cgttatcgga ataacggaac agggcagagt gcagcaccac
     3721 gttgccgcca aagaaagcaa aggcgttaat ttcgtcgttg ttgatcagaa agaaatgaaa
     3781 cggtgtctta accgaattgg catgcgaaac cagacgcata cccagcgaat taatatattg
     3841 cgttaacagc gggtcattaa ttaacggcgc gctgccgcgt agctggcgga cataatagtc
     3901 gcccatctgc atttcctggc caatggaaag cgtgcttcct gcggaggttc ccatatccgg
     3961 caaggtgtct gcgctgtcgg caaacgccgg ggctacctga ccaatggtca tagcagcaat
     4021 gagggttgca accaggtttt ttttcaactg cctgaacata acctctatcc tgtatttctg
     4081 aagatagtca tttgaccgat actgtgaaat atcgttccca ccgcgccgcc tttgagtgta
     4141 acggcgaaga ttagcccact caaatgactt ttcagtttcc tgaatccgtc ataaaaagcg
     4201 aagtcttttt tgtgacattt cgatacaatt gcggatcgca atcccgctct tgaggttcag
     4261 ggaaggtctt tatgctcgaa atgttgatgc aatggtatcg ccgccgtttt agcgacccgg
     4321 aagcgattgc cttgctggtt attttagttg ccggatttgg cattatcttt ttctttagtg
     4381 gcctgcttgc tccgttgctg gtggctattg tgctggccta tttgctggaa tggccaaccg
     4441 tgcgcctgca atctattggc tgctcccgcc gctgggcgac gtcgattgta ttggtggttt
     4501 tcgtcggtat attgctactg atggcgttcg tggtactgcc tatcgcctgg caacagggca
     4561 tctacttaat ccgcgatatg ccggggatgc tcaataagct ttctgacttt gccgccacgt
     4621 tgccgcgccg ctatccggcg ttgatggatg cgggcattat tgatgcaatg gccgaaaata
     4681 tgcgcagtcg gatgcttacc atgggcgatt cggtggtgaa aatttccctc gcctcgctgg
     4741 tcggtttgct caccatagct gtctatctgg tgctggtgcc attgatggtc ttcttcctgt
     4801 tgaaagacaa agagcagatg ctgaacgccg ttcgtcgggt gctaccgcgt aaccgcggac
     4861 tggcaggaca ggtgtggaag gagatgaatc aacaaatcac caactatatc cgcggcaaag
     4921 tgctggagat gatcgtggtg gggatcgcca cctggctggg gttcttactc ttcgggctga
     4981 actattcgct gctgctggcg gtgctggtcg gcttctcggt tcttattccg tacattggcg
     5041 catttgtggt gaccattccg gtgattggcg tggcgctatt ccagtttggt gctggcacgg
     5101 aattctggag ctgcttcgcg gtgtatctga ttattcaggc gctggacggc aacctgctag
     5161 tgccggtgtt gttctccgaa gcggttaacc tgcatccgct ggtgattatt ttatcggtgg
     5221 tgatcttcgg tggtttgtgg ggattctggg gcgtattctt cgccattcca ttggcgacgc
     5281 tgatcaaagc cgtgattcac gcctggcccg atgggcaaat cgcgcaagaa taatcttcag
     5341 cccgacgaac tgtttcgccg ggctgatttt tagggttcct gacgtaaata aatagcccga
     5401 taacaaatac tcaccagcac tgcaccgccg ataatattac ccagcatcac cgggagcaga
     5461 ttggcggtaa taaaatggct gatcgtcagt accggaaaat ggtctgcgct actgtgcgcc
     5521 agttgccaga aggaggtagg ggcgaaatgg cgaatggcaa tggcgaacgg aatcacaaac
     5581 aaattggcga tacagtgctc aaaaccactg gcgacaaaca gggtgatggg caaaatcatg
     5641 gcgacgattt tgtcgcataa cgaacgtccg caataactca cccacagcgc caggcaaacc
     5701 atcagattgc acataatgcc gaggctgact gattcagtaa atgtatgatg cattttgccc
     5761 tcggcgcagt gtaaaaccgc gactccccac atcgcgtttt cactcatcac cagcccggaa
     5821 aaccagatta acaaactgaa acaggcaata cctgccagat taccgcaggc caccagaagt
     5881 gcattaatca gccatgttcg ccaactaata acgccccgac tttttgccat taccgtcatt
     5941 accgacgagg tgaacaggct ggtgccgcaa accgccagca aaataaagcc aagtgtaaag
     6001 caaaggccgc ccaccagatg ggttaatgcc tgtgaagacg gggcatcggc gataacggta
     6061 aggtaaaaag taaatccgat cgccataaac acgccagcgg acatcgccat taaaaagacc
     6121 gacactttac tgcgggcaat tttatgggcg gcaatccgtt cagcgattgc cgcttttctg
     6181 gcgctcaact gcaagtcgaa agagagtttg tttctcatgc atactcctgc aaaaacagat
     6241 tacaacacct cgcgaaccga gatccccagc cgctgcattc gtgacagcag cgtggtgcgc
     6301 ttcatcccta atcgcgtcgc cgcgccacgg ggcccggcaa caatgccatt ggtttctcgc
     6361 aatacctgaa caatgcgctg acgttcttct tcgtcattct ctggcgtcgt cgggtgcagc
     6421 aactgcgcca ttgaactgcg aagcgctgaa tcttcattta gcgtcggcag taaacggctt
     6481 tgtcggacat taagatgtaa attcagactg ttaccgcgag tcagtagtac cgctcgctca
     6541 atcacgtttt ccagctcgcg cacgttgccc ggccagtccc acgacatcaa ctggcgtagc
     6601 gcctcggtcg ggatggcgtc aattgcgcga ttcatatggc gcgccatttt ttgcgtgaaa
     6661 tgctttgcta aaagagggat atcttccgga cggtcgcgca gtggcggcag ttccagtggg
     6721 aagacattca ggcgataaaa gagatcgctg cgaaactggc gatcttcaac catttgccac
     6781 aaatcacggt tggtggcggc aatgactctg acatttaccg ggatcgttct actcccgccg
     6841 agacgctcaa tctcccgttc ctgcaatacg cgcagcagtt taggctgaag ttctaacggc
     6901 agatcgccaa tttcatcgag aaacaacgtg ccgccatcgg caatttcaaa acgaccacga
     6961 tgggtattaa tcgcaccagt aaatgcccct ttgtcatgac cgaataactc actttccaga
     7021 agactggcgg ggatggcagc gcagttgatt ttgaccagtg gcttgtcgcg tcgtgggcta
     7081 agttgatgga tcgctctggc gatcacctct ttgccggttc cggtttcacc acaaatcaac
     7141 accgtactgt cgctcttcgc cacaatatct acctgctgga gcaaatcttc cattgcctgg
     7201 ctttgataga taatgtcacc gacgcccaga ttcgaaagga gctgctcgct aagctggtgg
     7261 ttttcttgct gcaagctttc ctgcaaatcg gtcatgctgc gccaggcatc ggcattgcca
     7321 acggcaatag cgatgcgatc ggcaatatgt tgtagtagct ggcagttttc ctcactaaag
     7381 agagtggaag aggtatgcgc cagcaacaat gcgcccggtg tatggttgcc aaaggtgagc
     7441 ggtataagga gcgcagattc gcagccgtta gatgcaagta agtgtaataa cgggtcgcgt
     7501 tgccagagaa acagatcgtc tgcacggtgc gtcaaggtcg gttgtcgggt ttgtagcgtt
     7561 tgtgtcagca atacactttc gccaggcata cagcgtggca gacacgcaca atgtgaggca
     7621 gaaagatcgc tgcaccacag gctgaacttc tcgttctttc gatgatcgcc cagtaccata
     7681 ctgactgaag ccagaccgaa aaaatgatgg atctcacgag cgacgtcagc gatcagatcg
     7741 tcgagatcaa gatgtgacaa cactgcatta gtgatatcca ccagtacccg atgatgatcg
     7801 cgatctttct ccagcaaacg cagcgccacc tgctgttctt catcattgag ccatgattgc
     7861 agaaccgggg tgattatccg cgccagccag ttaaacagtg tgcaatcctg gggagtaaaa
     7921 cgactgccgt gggtgcgaat aaagcgtagc tccgccattc cactactgcc gagaaggacg
     7981 gcgtggcgcg aataatcgac catctcactc tgatactctg ttgttgccgt cacatcgctg
     8041 ccatcatcat ggtagcggta atagaagttg cttttgagaa catgcagttc aataagttgt
     8101 accgggctga acggcggcgg cagcgggatg aaggcgcgta ataaagacgc cttgccgtgt
     8161 ttctgcgcta accgctccgc gagcattccg tttaccgctt caattgttat tccttgcggc
     8221 ggggcaaaca tcgcctcgtc tgacatagcc atcaacagtc cttattaatg agaaatcctt
     8281 ctcacgcaat ttttctggcc atcagataga tggccggttc ctgcacaatt tcatccaaca
     8341 gatgaatgaa ttgaatgcta cgcgcctgcc acgccgacat cgcgtcatct gccagtggcg
     8401 cgaatgcccg ggtcaacagg gcggtgtggc tggcgtcaat aacaatctca ccaaaggtca
     8461 tcacccccag catcttacgt cgggcttgct cctcttcgac cagtgcaagc caatcttcgt
     8521 attccgtcag tgggcagcgg aaggcgacac tcaggcaatc aatcacgcca acgtgatgac
     8581 cgatagccag cgagtaatac attacctgag agctgtgttc cggcatctcg tcgctactgg
     8641 cgacaaactt ttttcgcagc gtccagaaaa caatttccct gcactcctca gccatgtaac
     8701 ccccggagaa tgcgaaagag ttgctgaacg atactattga gacgcggatc gtcagcttcg
     8761 cgcaaccagg tgttaacccg atttcctgta ggatcgtcgc ttaaatggcg caggagccga
     8821 tcgcaaattt ctcgcccctg acgatagccc gccagccgcc gcgcttctcg ctcaacggcg
     8881 atacgctgtg atggcgggat ctgcggccac agcggttgca tggtcacccc ggtgggatcg
     8941 cgataatcca ccgcgtgaat cttctgttgc agcaaaccga gcgccacggc gaaaccgtga
     9001 atggtggcgg ccggtgttgg cgggcagccg gggatccaaa catcaatggg gacaatggtg
     9061 tcgctgccgc cccagacgct gtagagatcg tggaaaatac cgccgccgac accacacgcg
     9121 ccgtaggaaa cacagatttt atgatcgggg gcagactcat atgcccgaag tgcaggcata
     9181 cgcatcgctc gggtgactgc gccagtaaat aacaaaatat cggcgtgacg tggtgatgaa
     9241 acaaccttaa tgccaaaacg ttctgcgtcg aatacgggtg tgatggcggc aaaaatttcg
     9301 atttcgcagg cgttgcagcc gccgcaatcg acgcgataaa cgtaagccga gcgacggata
     9361 tcctgtagca aatgctgctt tatcttttgc gtttgctcgt ccagcgttat gggctggctg
     9421 acatgttgcg taagcactgg actcatagct gctccttagc caccagcggt tcatctgtgt
     9481 cgtcgttgat cagcgtcgcg cgttgtttgc actccgggca gacgctcgct tgcgcccata
     9541 acatttcgcg gttttgtggc gcattttgtt gctgtgccaa caattcagca gccagtgcga
     9601 cggttttttg cggggcaaac gggcgttcgc aacggctgca acgttgtaga tggaacgtcg
     9661 cgcgggtata gagatcggct ttattggtga cggtcagttc aaagttattg gtaagctgaa
     9721 tggctctggt cgggcacact tcttcacaac gtccgcagta aatacaacgc cccagataga
     9781 gctgccaggt gcgcgtattt tgctggtcgt cggtctggat agtcagcgca tttgccggac
     9841 aagcacaagc gcaggcaccg caggcaatac attggctggg catcaggtcc ggtttcccgc
     9901 gaaagcctgg gctgacctcc agtggcgcga agggatattt caccgtcgcg gttccggcgc
     9961 gcataatagt tttcagtaac ttcagcatct tctgtcctta cttcagcggc gaacggttac
    10021 gatcaatgcc gtagcgttcg atctctttat acggcacggt ttttgactgg cgcttgcgca
    10081 catcaaccag cgtcacacgg tcggtacagg agtagcaggg atcaaggcta ccgataatca
    10141 gcggtgcgtc agaaacggta ttgccgcgca gcatgtaacg cagcactggc cagttggcgt
    10201 aggtggcggc acgacagcgc cagcggaaca atttttggtt atcaccgagc atgctccagt
    10261 gcacgtcttc accacgtggc gcttcaacaa agcccagcgc gaatgcgtga ggtttatagc
    10321 taaagccttc ggtcagcagt ggggtatccg gcatgttgtc gagggcaaat tccagcattg
    10381 ccagcgaatc aaacgtctct ttgacacgga ccatcacgcg ggagaagaca tcgccgccgg
    10441 taaaggtaaa cagcgttttg ggaatattgc cgtagtcggc gtaggggtga tcaaagcgca
    10501 aatcacgggc aaaaccactg ccgcggatca gcggcccgac agggctataa tcacgggcga
    10561 tttgtcggtc gagaatgcca atgccctgag tgcgttgttc catattcggc gtggcaagca
    10621 gcatctctac cagctccgac acgtcggcgc gcatctcgcg caccagtttc agcgtttgca
    10681 gacgttgctc tttgagaata tcgcggcgaa caccaccaat cagattcaga ccgtaggttt
    10741 tacgcgaccc ggtcagcaat tccgccatcg tcatcgactt ttcccgcacg cggaaaaatt
    10801 gcataaagcc ggtatcaaat ccaacaaaat ggcaggagag gccaaggttg agcaaatgac
    10861 tatgcagccg ttcgacttcc agcagaatcg agcgaatagt atgcgctcgt tgcggcacct
    10921 caatccccag tgcattttca accgagttgg tataggccac actgtgggca aaaccgcaaa
    10981 tcccacacac gcggtcggat aagaaggtca cttcgttgta gcccatccgc gtttctgcca
    11041 gtttctccat gccgcgatgg acataaaaca ggcggtaatc agcatcgaca atttgctcgc
    11101 catccacaaa caagcggaag tgacctggtt catcggaggt gatatgcagc gggccgacag
    11161 ggataacccg cgcatcgctg ttgccctcgt tgataaacgg atacgtttcg gtatcagtcg
    11221 tcggttctgg gcgcagtcga taatccatcg cgtctttgcg cagcggatgc atatcttccg
    11281 gccagtcatc gggcaacacc agccgacgct gatccggcag gccaaccgga atcagcccgt
    11341 acatatcgcg aatttctcgc tcgccccaga ccgcggcagg gacgcgcggt gtgactgacg
    11401 gaaactcccg actgtcggca tcgaccagtg ccttcaccac gatccagcat ttttcggccc
    11461 cttccataga aagggcataa taaaccgcgt aatgaccgtt aagtgtccgc tcgtcgttgc
    11521 caaacaggac tggaagccag ccatcatgtt gataataaag ataatgcaca acgtcaggca
    11581 gcagattgat tttcaccgta atggtgacct gttcaggcgt ttgtcgctct tcatccagca
    11641 ccgcgccggg gaactgcgtt ttcagggcgg cgagaatcgc ttcgccacga tttgacgatg
    11701 aattaacgtt cactgtgttt ctccgaaaca gatgcggtgc ctgaaggcaa aaaatcatgc
    11761 caggtgctac gttgtgcagg caggtcgtgc gtccctgaga ggactatagt ggaagcgccc
    11821 gccaggatcc tgatgacagg ttgtggaata tgcgttccca tcgccagcat catgaccagc
    11881 agaatcacca ttggcgaggt ggtcaaccag ccaagttcac cccggttaac ggcctgcggc
    11941 ggtttcgcca ttaacacccg cgcagccatc cgtaccaggc ccgccagcac cagcgttaac
    12001 agcaataaca gcaggacgat aagcagcaga tgattacgcg ccagtccggc ggtaacggtc
    12061 ataaattcgc taagaaaaat gttgaagggc ggcatcccgg ccagcgccag cgcaccgccg
    12121 ccaaacaaca cggcggtaaa tggcatgatt ttaagcattc cgcagacgac gttgagatcg
    12181 cgcgtgccgt acttgagcag tacattgccg gaaccgcaga acaacagcgt ttttgccaga
    12241 ctgtggttta aggtgtgcag cagcgcggca aaaattccca gcggcccgcc aatgcctagc
    12301 gccaccgcga ccagtcccat gttctccacg ctggagtacg ccagcagacg cttaatgtcc
    12361 cgctgtacca gaatgaaaaa tgccgccacg gcaaccgaca acatgccgaa gatgagcaac
    12421 aaccggttgg ggaaatcgct gccgatggcc tggcaaataa tgatgtaata gcgaatcagc
    12481 accaacagcg cgcagttcag caatacggcg gagagcaggg cgctgaccgg actcggcgct
    12541 tcactgtgag catctggcag ccaggcgtgc atggggaata gcccggtttt agtgccaaag
    12601 ccaattagca cgaacacaaa ggccaacagc attaatgttg ggtcaagcaa ggacgattgc
    12661 ttaagaacct cgctccagaa tatcgccatt tctgcctgcg gcataacgct ggcggcgttg
    12721 gcgtatacca gcacggtacc gaacagacca aaagcgacac caacagtaca aataatgatg
    12781 tacttccatg cagcttccag cgatgaacgc tgaccgtaaa tgcctaccag aaacgccgag
    12841 cttaaggtgg tggcttcgat cgccgcccac atcacaataa ggttattgct ggtgacaacc
    12901 agcagcatgg tgaacaaaaa cagatggaag aaaccgtagt aatcgcacag cgtaacgggt
    12961 gaaagctcgc cgtgtgccac ttcatgacgc atgtagccaa tcgagtaaac accggtgaga
    13021 aagccaatca caccaagaat ggcgaggaac aaaccgccca gaccatcaat atgaagccac
    13081 agtcccgcag cgaatatttc tcccgcatca gcggcagttt ggaccaccca cagtgccaga
    13141 atcagtagca gtgtgatccc taagctatgt aatactgtca ccgcgagagt cgcagagtgc
    13201 ccccgtttcc gacaggcaaa acagagcagc gaaaaaagca gcggcgtgag caggagtaaa
    13261 gcgaacatca cagaataact catctcatta tcccttcagc gcggtcaagt tattcacgtc
    13321 cagcgtgccg tgggtacgcc atatttttct tgccagtaac accatcacaa tgacggcgaa
    13381 gatggcgtcg gtagcgatac caatttccac cagttccggt gcacgccagg ctagaagcgc
    13441 cagcaccaga tgggagccgt tttccatcag gcagtaacca aaaatttgcc gcaggatatt
    13501 gcgctggctg acaatgcaca gcaggccaag cagaaaatga cctaacgcca ccgccagcgc
    13561 cggtttcagc ccggtagcca tcggtagctt cacgggctga acgacaaatg cgcaaagcag
    13621 gacaattaac gccgcgagca gtgtcatcat tgccggaccg aataacgctt tttccgggat
    13681 gttctgggga atatttcgtg cagcgtaagt catgattaac ggtaccagca gcactttggt
    13741 gataaaggcg ctggcggacc agatcagcag ttgctctgcg gcgaacaggc acgagagagt
    13801 ggcgaaaata gacaccagca ccagcgactg gcaggcgtaa aatccgcatg acaggcgata
    13861 gcttttgacg ctaatcacaa acagcgatgt cagcatcatc agtcccgcca gattatttac
    13921 gatcatagaa ccggtcattc atcatctcct tactgcaacc aggtcgcggc gattacgctg
    13981 gaaatcagcg acatcacaat cagtacaatc aacaccaggc gcattgatcc tggcagcggt
    14041 gcggcatcgg cgacggcctc gctcggtttg ccaggcacga cgcgaccaaa ccagcgaata
    14101 aaccaggcga aactggcgac cgattcaatc atcagcagaa tcatggcggg cagcaggatc
    14161 cagtactcca ctgacaacgc aaaaccggcg gcaaacagcg ggaatttact gaagaagccg
    14221 ttgaacggcg gtacgccggt aatcgccagc gctgccacgc agaaacccac gcctggcaac
    14281 ggcagggtgt gcaatacgcc acgcagacgc ggcaacaagc gcgtgccgca gctgtaactc
    14341 agcgcacctg ctacaaggaa aaacaggctt ttagcgaacg cgtggttgac gatgtaggcg
    14401 atgctgccct ccagcgccag ccgcgagccg aagatggaga gcgacaagcc gaaaaacatc
    14461 cagccaagtt gagtgatggt cgaccaggcc agcaaccgct tcatatcctg ctgtggcaaa
    14521 tacatcagga agccataaag aatggtgacc agtgccataa ccatgccaac gccgccaatc
    14581 acatgcggga tattgccgcc gtcgataatg gcacgggcaa aaatgtaaac gcccactttc
    14641 accatcgatg cggcgtggag ataggcgctg atcggtgttg gcgcttccat tgcatccggt
    14701 agccacgctt gcatcggtag ctgggccgat ttcccccacg cggcaaacag gatgccacca
    14761 taaaccagat agcgtgcgtc gccgtgtaac tcgctcatcg cgctaagcgc aaacgttccg
    14821 gtctgcaaaa acagcgtggc ggcggcaaga tacaacccca acgaaccgat atgggtaata
    14881 agcaacgctt ttagtgccga acgctgcgct ttatcgctct ggtaataact gatcaacgcc
    14941 caggagcagc cgcccgtaat ttcaaaaaac aacaactgac cgagcagcgt cgaagagagt
    15001 accagtcccg ccatcgcgcc gataaacagc agcaaaaatg cgtaataacg attcgtgccg
    15061 ttatgcgggt gttcgcgatt tttatccgtc agataacccg tggagtagat cgtgaccagc
    15121 aaaccgagga acaccaccac aaacagaatc agcgtactca cgcgatcaat gaccaggcca
    15181 aacaacgcca catcccccac gttgaccaac gtcaacgtga cggcaacttt atcggcctga
    15241 taaaacgcgg agatcagtga caacatgcac agcgtggtca gcgcggcgaa caaaaccccc
    15301 cattcggcgg cccgacgttg tggcgaaaac gaaacgacca gtgcgccgat aaaaggcagc
    15361 aataacgtcg tcagagcaag attttccata ttccgtcagt gctccttaca gaccggttaa
    15421 ccagaagacc catgcaagcg cagcaaggct gaagccaagc caggtcacat ggtgagtgag
    15481 taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag gccagtacaa aaatcagcaa
    15541 aaccttgagc agcgtaacga caagtgaagt cagcaggcag gcgagagaaa gttcttgcgc
    15601 gcgcccaaag ggcagaaaca gggccacaaa cagtgctgcc atcacgatct gtttcagccc
    15661 cagccccaac ttcgccagcg ccagcccggc accggaatat tcggtcagcg ggccttcctg
    15721 taattcctgt tctgcttcag caacatcaaa gggaattttt cccatctcaa tgaagcaggc
    15781 aaaaccacag gccagtaacg ccagtacggt ggttagcggc gagttccagc ccgtcgccag
    15841 cgtcttgctg atcatctcga tatgcgtgga atccgctatc agcgccaata ccagcagtga
    15901 gagaatgagc atgggttcga ccagaatgcc gagcgtcaac tcgcgactgg caccgactcc
    15961 cgcaaacggg cttccggtat ccagcccgga aagagcaaag aaaaaacgaa acagggcaag
    16021 aagatagata agggtgatca gatcgccgcc gcccgcaaaa ggggaaacag taataaatag
    16081 tggtagggcc atcgccagca ccagcatgct gctgattaat acccacggca tcatgcggaa
    16141 catcagacct gaagatgtcg gcgcaacttc ctggcgttta aacagtttgt ggatatcgcg
    16201 ataatcctgc cagatccccg gtccgcgacg ggagtgcata cgcgcgcgta tctgccggga
    16261 aatccccgta aaaagcgggg ttagcgccag cagaatcacg gcctgtgcta acgcaaaaat
    16321 gatcagatat ccgtcgcaaa gagtttgtct catggtgatt tccttagacg gcaatagcga
    16381 ttagcagcac aaccagtgcg gcgaccacat acaggcaata gagacgaaag tcgccgcttt
    16441 gcagatgctg tatttctttg gccagccgtt gggtggcgct cacgatgggg cggatcaccc
    16501 gctcatccca gaagggttct gtgctctgag ccctggcggt gacgtgcgca agacctttgt
    16561 tcagcctcag cgtagggtcg agctgttgtc gtagacgaaa tagcgcacaa aagaccacac
    16621 gcagcggctg catcacgcca ttgcctgacg gggccatcgc attttcccag ccgtagccgc
    16681 atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg cgaacgacac catagccagt
    16741 aaaggccagg cagcaaaggc atcgccagta gtaacagcaa cagtaatgag ggagtgaccc
    16801 gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc gacagtgacg ggaggggtat
    16861 tggtaaacgc atgggcaatg tgcattatct tcggtgccag ccaactggca ctaagcgcaa
    16921 tgaatacgca gagtgccgcg agcagtagca tggcgacgat catcgtattt ggcacttcct
    16981 gtgcctcttc agcgtgtgta ctgcgcggcg ctccacagaa agtaataccg tacattttga
    17041 cgaagcacat tactgccagc ccaccagtga ctgccagcat caccatagca ataggacccg
    17101 caagttgtag cgctacggct tccacacgac ttagtgagaa cagcgactgc caggtgtacc
    17161 attcgctgat aaaaccattc agcggaggaa tggctgatat cgcgaggcaa ccaatcaggc
    17221 atgctgcggc tgtccacggc atccgttttg ccagtgcccc cattttttcc atgtcgtggg
    17281 tatgcaaacg cgaaataatc gcacccgcgc cgagaaacag cagccctttg aacagcgcat
    17341 ggttgagcag atgaaacagc gcgccgagca gtccaacaac ggtgagcagc gggtcgtgca
    17401 gtgacagacc gaccatcgcc acaccgaccg ccagcaaaat aatgccgacg ttttcgacgg
    17461 tactccaggc cagcagccgt ttgatatcct gttccgccag cgcatacagc acgcccagga
    17521 gcgcggagat tgcgccgatc gccatcacca gaatgcccca ccacagcggc aaacccgttt
    17581 gcgccagcag gtccatcgct actttcagga tgccgaaaat accgattttg accattacgc
    17641 cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg agcgcgtggc aaccagctgt
    17701 gcaacggcat catcccggct ttcgcgccaa aaccaaagaa ggccatcagg aacaccgccg
    17761 acgccagccc cggagaaagt gaaagcgtgc ggaaactggc aaaatcgagg ctgccgcttt
    17821 cgcgccccat cagcaagaag gcgatcataa tcagtaccga tccggcgtgg gcgataaaaa
    17881 agtagagcat gccagcgttg attgacgttt tatcctgcct ggcaatgacc agaaaccagg
    17941 aagacagcga catcatttca aacagcacga tgaaccaaaa agcgttgtcc atcaccagca
    18001 gggcaaccat cgatgcgatg aaaagattca taaagaagcc catcgccgcc gcgcctttgc
    18061 cctcgtattc gcgcatataa gtcaatgaat agagcgaaca aaccaccacc agcaaggaga
    18121 taaccagcac cataaatgcc gagaggctat ccattcgcag ggttaaatcg gcaaagttaa
    18181 aaggcgtggc aaaacgggcg actaaagtta cgcctgcatg taattgcgta attccgctga
    18241 tgatgccaat cacgccaccc accagcgatg tgatgccgga aagcttaata gccagtctgt
    18301 ccagaccgag taaaaacagc gaagccagac tggcaaacag atagaggatc agcgaccagg
    18361 ttaataattg cagggcatcc attagcgttg ctcctgagtg agggaaaggg gatcctcccc
    18421 gccattggca aaccagcttg cggcaaggcg ctgtttttct ttcatctgac gttgcaggct
    18481 atcgtcggtg atcagccgta acgcctgatt cgggcaagcg cgaacgcagg ccggaccttc
    18541 tggcaagaaa tcacacaggt cgcatttcac cgcgacggtc tggacacccg gttcccagcg
    18601 cagcaaagca tgttgggtcg gcgcgttttc ttcgccgtct ttgagtgagc catcagcctg
    18661 aaaaacatat tgcgccgggg cattcaccgg acggcttcct gaagcgctga ttgcaccaaa
    18721 tgggcagacc acggcgcaaa gcttgcaacc aatacaaagg ctttcgttga gttggatcgc
    18781 atcatccctc tgggagatgg cattgaccgg gcagacctgc aggcaagggg cttcctcaca
    18841 gtgatgacac acgacagggg cagtgagtgt tgacgtcttc gccagggcca ggcgcgggtg
    18901 ttgctgtaaa ccttgcgttt tatgcacgtc cgaacaggca gcgagacagg tattacatcc
    18961 tgtacaccac agtggttcgg ccaccacaaa gcggttcatg tcggctcctt gaaaatgata
    19021 tgaaacattg catttcatat tgcataaagg atgccagtct gattaattta atgtattcaa
    19081 taagttatga attaactgta ttctgaacgg tacaaaaatg atggtcatta acgttcagca
    19141 aaaaatgaat tcgacacccg tgtcgcaggg gaaagcgaga atgtgatctt gatcacgaga
    19201 agtgaggtgt gctgaccgca agcgcagcca gcacggaatg gagcaaagta atcaggcgtg
    19261 ttctttcagc cagttcagca caacgtcgtg gtgattgctg gttttgaaat cgtcaaagac
    19321 atgttcgatt ttgccatcag cgtcaatcag gaagctgatg cgatgaatac catcgtaggt
    19381 tttgcccatg aaggactttt caccccagac gccgaattgt tcgcacacct ggtggtcctc
    19441 atcagacagg agcgtaaagt taagcagctc tttttccgca aaacgggaga gtttttcggg
    19501 tttatcggtg ctgataccca gcacatcaac gcccgctttt ttcaactcat ccatgttatc
    19561 gcgtaagccg caggcctgta cggtacagcc gggggtcatg gctttcgggt agaaataaac
    19621 cagaacacgc tgtccctgga agtcggtcaa attaacttgt tctccgtctt gatccggcaa
    19681 gctaaatttc ggtgcgatat caccggcttt cagtggattc attacttaac tccatcctgt
    19741 tcatcatgtt gggaataatt gacgacgtta atactgcctt gtgcattgag ttctgtacat
    19801 agggctttga acgcttgctc aatatttgct gcgtccgcag atgcggggct gtgggcggtt
    19861 atctgaatat gcaactgcgc agcccgttca ttttcagcag gttgcgtgcg tgacaccagc
    19921 tccgcaatgt tcatatgatg cgcgtcgaaa agtgctgtga agcgttcaat taaatgcggg
    19981 gagtctgcca catcgacctg aacccagaca gatgctggca ttggcggacg cggacgcgcc
    20041 gtcgtgcgct tcatcacgat taaaagatcc agttctgcac ctttcaacgg taacgttgat
    20101 tcaatcagag taatggcatt ccatgaaccg gaaagcagca tgataaacgt gaactcttct
    20161 cccagcatcg ccaggcgact gtcttcaata ttacagccgc aactactgac atgacgggtg
    20221 atggtgttca caattccagg gcgatcggca cccaacgcag tgatcaccag ataatgttgc
    20281 gatgacagtg tcaaactggt tattccttta aggggtgagt tgttcttaag gaaagcataa
    20341 aaaaaacatg catacaacaa tcagaacggt tctgtctgct tgcttttaat gccataccaa
    20401 acgtaccatt gagacacttg tttgcacaga ggatggccca tgttcacggg aagtattgtc
    20461 gcgattgtta ctccgatgga tgaaaaaggt aatgtctgtc gggctagctt gaaaaaactg
    20521 attgattatc atgtcgccag cggtacttcg gcgatcgttt ctgttggcac cactggcgag
    20581 tccgctacct taaatcatga cgaacatgct gatgtggtga tgatgacgct ggagctggct
    20641 gacgggcgca ttccggtgat tgccgggacc ggcgctaacg ctactgcgga agccattagc
    20701 ctgacgcagc gcttcaatga cagtggtatc gtcggctgcc tgacggtaac cccttactac
    20761 aatcgtccgt cgcaagaagg tttgtatcag catttcaaag ccatcgctga acatactgac
    20821 ctgccgcaaa ttctgtataa tgtgccgtcc cgtactggct gcgatctgct cccggaaacg
    20881 gtgggccgtc tggcgaaagt aaaaaatatt atcggaatca aagaggcaac agggaactta
    20941 acgcgtgtaa accagatcaa agagctggtt tcagatgatt ttgttctgct gagcggcgat
    21001 gatgcgagcg cgctggactt catgcaatta ggcggtcatg gggttatttc cgttacggct
    21061 aacgtcgcag cgcatgatat ggcccagatg tgcaaactgg cagcagaagg gcattttgcc
    21121 gaggcacgcg ttattaatca gcgtctgatg ccattacaca acaaactatt tgtcgaaccc
    21181 aatccaatcc cggtgaaatg ggcatgtaag gaactgggtc ttgtggcgac cgatacgctg
    21241 cgcctgccaa tgacacctat caccgacagt ggtcgtgaga cggtcagagc ggcgcttaag
    21301 catgccggtt tgctgtaaag tttagggaga tttgatggct tactctgttc aaaagtcgcg
    21361 cctggcaaag gttgcgggtg tttcgcttgt tttattactc gctgcctgta gttctgactc
    21421 acgctataag cgtcaggtca gtggtgatga agcctacctg gaagcggcac cgcttgcgga
    21481 gcttcatgcc ccggctggaa tgattttgcc ggtgacctcc ggtgattatg caatcccggt
    21541 gaccaacggt agtggtgctg tcggtaaggc gctggacatt cgtccaccag cccagccgct
    21601 ggcactggtt tctggcgcgc gtacccagtt cacgggcgat accgcttcat tgctggtgga
    21661 aaatggtcgt ggcaatactc tgtggccgca ggtggttagc gtgctgcagg cgaaaaacta
    21721 caccatcacc caacgtgatg atgctggtca gacactgacc accgattggg tacaatggaa
    21781 ccgtctggac gaagacgagc agtatcgtgg tcgttatcaa atctctgtta agccgcaggg
    21841 ttatcagcag gcggttacgg ttaaactgct gaacctggaa caggcgggca aaccggttgc
    21901 agacgcggct tccatgcagc gttacagcac ggagatgatg aacgttattt ccgccggtct
    21961 ggataaatct gccactgacg ccgcgaacgc tgcgcaaaat cgtgcctcca ccactatgga
    22021 cgtacaaagt gcagctgatg acaccggttt accgatgctg gtcgtacgcg ggccgttcaa
    22081 tgtggtttgg caacgtctgc cagcggcgct ggaaaaagtg ggcatgaaag tgaccgacag
    22141 cacccgttcg cagggtaaca tggccgtaac ttacaagccg ctgtctgaca gcgactggca
    22201 ggaactgggc gcgagcgatc caggcctggc atccggtgac tataaactgc aggttggcga
    22261 tttagataac cgcagcagcc tacagttcat cgatccgaaa ggtcatactc tgactcagag
    22321 tcagaacgac gcgctggtag ctgtcttcca ggctgcgttt agcaagtaaa aatacagggc
    22381 tggagtcatc cggccctttt ttctgatatg atacgcaaac gtgtgcgtcg gcagaaaaat
    22441 gcgattttag cggtaattcg cacaaaattt gtttgtcgga cgtaggtcgg ataaggcgtt
    22501 cttgccgcat ctgacaaaac atccggcaca ccagacagca aaagatttta aaacgttaat
    22561 tcacacccag gagtgataaa gatgcaaaag caagctgagt tgtatcgtgg taaagcgaaa
    22621 accgtataca gcacggaaaa cccggacctg ttggtgctcg aattccgcaa tgatacgtca
    22681 gcaggggatg gcgcgcgcat tgagcagttt gatcgcaaag gtatggtgaa caacaagttc
    22741 aactacttca ttatgagcaa actggctgaa gcgggtatcc cgactcaaat ggagcgtctg
    22801 ctctccgaca ccgaatgtct ggtgaaaaag ctggatatgg tgccggttga gtgtgtcgtg
    22861 cgtaaccgtg ctgctggctc tctggtgaaa cgacttggaa tcgaagaagg tattgagctg
    22921 aacccgccgc tgttcgatct gttcctgaaa aacgacgcca tgcacgatcc gatggtcaac
    22981 gaatcttact gcgaaacctt tggctgggta agtaaagaga acctggcgcg tatgaaagag
    23041 ctgacctaca aagcgaacga cgtgctgaaa aaactgttcg atgatgctgg tctgattttg
    23101 gtcgacttta aactggagtt tggcctgtac aaaggtgaag tggtactggg cgatgagttc
    23161 tccccggacg gcagccgcct gtgggacaaa gaaacgctgg agaaaatgga caaggaccgt
    23221 ttccgccaga gcctcggtgg cctgatcgag gcctatgaag ccgtcgcccg ccgcctgggt
    23281 gtacagctgg actgattttt ctgctcatca tcttgccgtg cagctggcac ggcaagacaa
    23341 ccgctctcat aagatgtgca ttgaacgctc attcctccgc catttcatcc cgctttcctg
    23401 tggtaatctt cctctgaacc acctacgatc atttctataa tgaatatatt gttgaggtat
    23461 ctatgcgttg gcaagggcga cgtgaaagtg acaatgttga agacaggcgc aacagctctg
    23521 gtggtccgtc tatgggcggt cccggttttc gcctgccaag cggtaaaggc gggctgattt
    23581 tactgattgt cgtgctggtt gcaggctact atggcgttga tttaaccggg ttgatgaccg
    23641 gacagccggt ttctcaacag caatcaacgc ggtcaattag cccaaatgaa gacgaagccg
    23701 caaaattcac ctcggtgatt ctggcaacca cggaagacac ctggggacag cagttcgaga
    23761 agatgggtaa gacctatcag caaccgaagc tggtcatgta ccgtggaatg acgcgcaccg
    23821 gatgcggggc gggccagtcc attatgggac cgttctattg cccggcggat ggcacggttt
    23881 atatcgatct ctccttctat gatgacatga aagacaaact tggcgcggat ggcgattttg
    23941 cccaggggta cgttatcgcc catgaagtcg gtcatcatgt gcagaaactg ttaggcatcg
    24001 agccgaaagt tcgtcaactg caacaaaacg cgacgcaggc ggaagtgaac cgcttatctg
    24061 tgcgtatgga actccaggcc gactgttttg ccggtgtctg ggggcatagt atgcagcagc
    24121 aaggtgttct ggaaaccggc gatctggaag aggcgctgaa cgcggcgcag gccatcggcg
    24181 atgaccgttt acaacagcaa agtcaggggc gagtagtacc agacagtttc actcatggca
    24241 cttctcagca acgctacagc tggtttaaac gtggtttcga cagcggtgat ccggcacaat
    24301 gcaatacttt tggtaaaagc atttaacttc cggggcaggg atggctgaac tgactgcgct
    24361 tcacacatta acagcgcaaa tgaaacgtga agggatccgc cgcttgctgg tgttgagcgg
    24421 ggaagagggt tggtgttttg atcatgcgct taagttgcgt gatgccttac ctggcgactg
    24481 gctgtggatt tcgccgcagc cagtcgctga aaaccactgt tttccctcgg cgctacaaac
    24541 tttacttggg cgcgaattcc ggcatgcggt attcgacgcc cgccacggct ttgatgccgc
    24601 tgcctttgca gcacttagcg gaacgttgaa agcgggaagc tggctggtgt tgttactccc
    24661 tgtatgggaa gagtgggaaa accaacctga tgccgactcg ctgcgctgga gtgattgccc
    24721 tgaccctatt gcgacgccgc attttgtcca gcatctcaaa cgtgtactta cggcgaataa
    24781 cgacgccatc ctctggcggc aaaaccagcc gttcacgttg gtgcattttg ctccccgtac
    24841 tgactggcac cccgcgaccg gcacaccaca accagaacaa cagcaactct tacagcagtt
    24901 actgaccatg ccgccgggcg tggcagcggt aacggctgcg cgtgggcgcg gtaaatcggc
    24961 gttggcaggg caactcattt ctcgtattgc gggcagtgcg attgtcaccg cgcccgcaaa
    25021 agcggcaacg gatgtactgg cacaatttgc gggcgagaag tttcgcttta ttgcaccgga
    25081 tgccttgtta gccagcgatg agcaagccga ctggctggtg gtcgatgaag ccgcagccat
    25141 acctgcgccg ttgttgcatc aactggtatc gcgttttcct cgaacgttgt taaccactac
    25201 ggtgcagggc tatgaaggca ccggacgtgg ttttttgctg aaattttgcg ctcgctttcc
    25261 acatttacac cgttttgaac tgcaacaacc gatccgctgg gcgcagggat gcccgctgga
    25321 aaaaatggtt agtgaggcac tggtttttga cgacgaaaac ttcactcacg aaccacaagg
    25381 tgacatcgtc atttctgctt ttgaacagac gttatggcga agcgaaccag aaacgccgtt
    25441 aaaggtatat cagctattgt ctggcgcgca ctaccggact tcgccgctgg atttacgccg
    25501 catgatggat gcaccagggc aacatttttt acaggcggct ggcgaaaacg agattgccgg
    25561 agcgctgtgg ctggtggatg agggcggatt atctcaagaa ctcagtcagg cggtatgggc
    25621 gggttttcgt cgcccgcggg gtaatctggt ggcccagtcg ctggcggcgc acggcagcaa
    25681 tccactggcg gcgacattgc gcggacggcg ggttagccgg atagcagttc atccggctcg
    25741 tcagcgggaa ggcacagggc ggcaacttat tgctggtgct ttgcaatata cgcaggacct
    25801 cgactatctt tcggtgagtt ttggttacac cggggaatta tggcgtttct ggcaacgctg
    25861 cggttttgtg ctggtgcgaa tgggtaatca tcgtgaagcc agcagcggtt gctatacggc
    25921 gatggcgctg ttaccgatga gtgatgcggg taaacaactg gctgaaggtg agcattaccg
    25981 tttacgtcgc gatgcgcaag ctctcgcaaa gtggaatggc gaaacgcttc ctgttgatcc
    26041 actaaacgat gccgtccttt ctgacgacga ctggcttgaa ctggccggtt ttgctttcgc
    26101 tcatcgtccg ctattaacat cgttaggttg cttattgcgt ctgctacaaa ccagtgaact
    26161 ggcattaccg gcgctgcgtg ggcgtttaca gaaaaacgtc agtgacgcgc agttatgtac
    26221 cacacttaaa ctttcaggcc gtaagctgtt actggtccgt caacgcgaag aagccgcgca
    26281 ggcgctatac gcacttgatg atgttcgcac tgagcgtctg cgcgatcgca taacgcaatg
    26341 gcaatttttt cactgactcc ttcaattttc tcccatggtc attgtgagtc agcggcgtaa
    26401 aattaatgac atcagttaag gagttcgcca tgaaacatga ccattttgtt gttcaaagcc
    26461 cggataaacc tgcgcaacag ttgttactgc ttttccacgg tgtcggggat aacccggtgg
    26521 caatggggga aataggtagc tggtttgcac cgctgtttcc tgatgcgctg gtggtgagtg
    26581 tcggtggcgc ggagccgagc ggtaatccgg cggggcgtca gtggttttcg gtgcagggta
    26641 ttacggaaga taatcgccag gagcgtgtgg atgccatcat gtcgacgttt attgaaacgg
    26701 tacgctactg gcagaaacaa agcggagtag gggctaacgc cacggcgcta atcggtttct
    26761 cacaaggcgc gattatggcg ctggagagca ttaaagccga accgggcctt gcttcgcgcg
    26821 tcattgcctt caacggacgc tacgcgagcc tgccggaaac cgcgtctaca gccacgacaa
    26881 ttcacttgat tcatggcggt gaagatccgg ttatcgatct ggcgcatgcg gttgcggcgc
    26941 aggaagcgtt aatcagtgcc ggtggtgacg tgacgctgga tatcgtggag gatttaggcc
    27001 atgcgatcga caatcgcagt atgcaatttg ccctggatca cttgcgttat accattccga
    27061 aacattattt cgacgaagcg ctaagcggcg gtaagcctgg cgatgatgat gtgattgaaa
    27121 tgatgtaata atagccgggt aggcctgata agactgtatc aggcatcatg gccggatgtg
    27181 gcgtgaacgc cttatccggc cgctcagctt acttcttcgg ttgatccttt ttcggccagt
    27241 cgtcgtcatc gtcccactta tcgttgaaat cacgatgcgg gggaagctcc ggcttgttgg
    27301 cgagaaattt cttatggtcg acgcgcttga gatctttaat cacgttcagc aggacgccta
    27361 ccaaaaagac aatcaccaga atccaccaat atttagccag ccagtccatg cttatttcct
    27421 cttgcagaac cactcatcag gcgacgagct gttccatgat acgttgatac atacgggcaa
    27481 gtagctgcag gtcggcagcg ttcacacatt cattaatttt atgaatagtg gcattgaccg
    27541 gcccgagttc caccacctgc gcccccatgc gggcaataaa gcgcccgtcg gacgttccgc
    27601 ctgtggtcag tagctgcggt ttaatttcat tatagtgctc aaccgcgtta acgaccgcat
    27661 ccaccagttt accgcgcgcg gtcaaaaatg gctgcccgga aagccaccaa tccaccgtat
    27721 agcgcagttg atgtttttca agcagggcaa gtacctgcgc tttgatcatc tcatcagtca
    27781 gttcggtgct gaagcggaag ttaaactgca caaacagttc acccggaata acgttgttac
    27841 tgcccgttcc cgcctgaata ttggcaatct gcatactggt cgccgggaag aattcattgc
    27901 cctgatccca ctcaatagcc actaattcat taaggaaagg tgctgcgcga tgtaccggat
    27961 tgtcagccag atgtgggtag gcaacatgcc cctgcacgcc atgaatggtg aggttgcagg
    28021 ttaacgatcc gcgacgacca tttttcacca catcaccaac cacttcgata ctcgacggtt
    28081 cgccgaccag gcagtaatcg agacgctcat tacgcgccat taacgcttcg acgactttta
    28141 ccgtaccgtt atgggcactg gcttcttcat cagaagtgat cagaaatgcc agtcgccccg
    28201 tatggttggg atgttgtgcg acaaagcgtt ctgccgctac caccatcgcc gccagcgagc
    28261 ctttcatatc tgccgcaccg cgcccgaata acatgccgtc acgaatggtg ggttcaaacg
    28321 gcggattgat ccaacgatcg gcgtcgccag gcggcaccac gtcggtatgc ccggcaaagg
    28381 ccaacgtttc accttgccca cgccatgccc aaaaattctg cgtatcggca aagtccatgc
    28441 gttcaacggt aaaaccgatc gcctgcaaac gttcaatcaa caaagcctgg catcctgcat
    28501 catcaggact cagggaaggg cggcgaataa gctgttgtgt cagctcaata accgggcacg
    28561 acatagacta cacctcatgg aaaaaatgct gataactgga atcactgaaa cccagcagca
    28621 taggcttacc aggcgcgcag agcaatggac gtttgataat tgcaggcatt tcagtcatta
    28681 atgccgccgc agaggccgca tcgatgattt tattgcgggt ggtttcgtcc agtttacgcc
    28741 aggttgtacc acgggtgttg agtaacgctt cccagcctaa ttcgttgata aaaccgttca
    28801 ataattcgct gtccagccca tcgacgcggt aatcatgaaa acgatagtcg atgttattgg
    28861 cttccagcca acggcgagcc tttttaatgg tgtcacaatt tttgatgccg taaagtgtaa
    28921 ccataatgaa tcctttttcg atttatgaga ataaatactc atttaagggt gaaaacattt
    28981 tgcctgcaaa tgattatcag gaaatataac attatcgaag tattcaaatt aacaactatt
    29041 aaataatatt aaacattttt aacgtaaaca ttaagaatat tcatcttgtt atctgcctgt
    29101 attttaacca gaattaaatc agtattagat gccgtatgaa tatctcgaaa tgttgctgtg
    29161 tatcacatac aaatcatcac cgcaggacca tagtatttac ataaggtttg tcccccggag
    29221 gtagtcatcg cagcaatcga gtaaattagc ttcaccacgg tatattgttt ccgtagaata
    29281 cccataatta taagagaggt tgttatgatt gaacgtgaac tggggaactg gaaagacttt
    29341 atcgaagtta tgcttcgtaa gtaattcctg agacaaagta caaacagcaa gaacccgctg
    29401 agcaggttct taatcggaaa gttgaaaaag gcgacacatt ggcatgtcgc cttttttatt
    29461 gcttattccg ggcgcggctt tagcgggaag cggcggcgca ccagcacaaa gaacagcggc
    29521 acgaagtaaa tagccagaat agtggccgaa atcatcccgc ccattacgcc agtacccacc
    29581 gcatgctgac caccggaacc ggcacccgtg ctggttgcca ttggcaatac gccgaagata
    29641 aatgccagcg aggtcatcag aattgggcgt aaacgctgac ggcaggcgtg gagcgtcgct
    29701 tcaaacaggt cgtggccttt ttggttcatc tcgttagcaa actcgacgat caggatggcg
    29761 tttttcgccg ataaaccaat gaccgttaac aggcccacct ggaagtaaac gtcgttttcc
    29821 agcccgcgca tccaggttgc cagcagcgcg ccgataaccc ccagcggcac gaccagcatt
    29881 accgagaacg gcaccgacca gctttcatac agcgccgcca gacacaggaa taccaccagc
    29941 aaggaaatag cgtacagcgc cggagcctgc gcgccggaaa gccgctcctg atacgacatc
    30001 gccgtccact ccagaccaaa gccgtttggc agctgcttca ctaacgattc cataatatcc
    30061 atcgccgtac cggtactgac ccccggtgcg gcttccccaa caatctcaac cgcagaataa
    30121 ccgttatagc gttccagacg cggcgagcct gtctcccagc gtgaggtcgc gaaagcagag
    30181 aagggcacca tgccgccatc tttatttcgg acataccaga gattgatgtc atctggcagc
    30241 atgcgatacg gcgcagctgc ctgcacatag actttcttca cgcgaccgcg atccataaag
    30301 tcattcacat agctcgaacc ccaggcggtt tgcagtgtgt cgttaatatc gtcgatagca
    30361 acgcccagcg cctgagcttt acgctggtcg atatcaatct gcaactgcgg actgtcgtcg
    30421 aggccgttat ggcgcacacg ggttagctcc gggttttccg ctgccagcgc cagcaactgg
    30481 tttcgtgctg tcatcagcgc atcgtgaccc gctccagcgt ggtcctgcaa ctccatatca
    30541 aaacccgcag aactaccaag accgctaatt gctggcgggc tgctggcgat aacgcgagct
    30601 tctttaatct tgttaaacgc cttcgttgca cgctcgataa tggcaaacga ggtgccggtc
    30661 ttactgtcgc gttcgctcca gtctttcagg cggataaaca ttctcgccac gttttgcccg
    30721 ttacccccag ggccagaacc aacggtggca aacaccgaca tgatgttgtc tttttcatgg
    30781 gtgaagtagt atttctcgat ttgctcaacg actttcaggg tctgttgttg ggttgaaccg
    30841 ctgggcaact gtaccgaggt agtaaacatg ccccggtctt ccagtggcaa gaacgacgtc
    30901 ggcaaacgca ggaacaggaa caccatgccg ccaagcagca ggacataaat cacaatccag
    30961 cgcaggctac ggtggagaat tttcgccacc cctttttcgt agcgttcggc gttgcggtta
    31021 aacatctggt taaaccaggc aaaaaagcct ttttgcccgt gatgctcacc ctttttcagt
    31081 ggcttaagta gcgtggcaca cagagccgga gtgaggatca tcgctaccag tactgacagc
    31141 accatcgccg caacaatggt aatagagaac tggcgataga tggcaccggt ggtgccgccg
    31201 aagaaggcca ttggtacaaa taccgccgaa agcaccatcg caatcccgac cagtgccccc
    31261 tggatctgcc ccatcgattt acgtgtggct tcgcgaggag tgagtccttc ctcactcata
    31321 atacgttcga cgttttccac caccacgatg gcgtcatcca ccagcagacc gatcgccagc
    31381 accattgcga acatggttaa ggtgttgacg ctgtaaccga aggcgtaaag tacggagaag
    31441 gttcccatca acaccaccgg cacggcgata gtgggtatca gcgtggcgcg gaagttttgc
    31501 aggaacagat acataacgag gaaaaccaga gcgatagctt ccagcagcgt tttcaccacg
    31561 tcttcaatgg aggctttaac aaacgaggtg gtttcatacg ccaccttgta ttccagtccg
    31621 tgcgggaaat actgcgccag ctcgtcgaga cgattgagca ccagctccgc tgtcgccatt
    31681 tcgttcgcgc cggaggccag ttttaccccc aacccggagg ctggcttacc attgaagcgg
    31741 ctaagataat cgtatttctc cgcccccatt tcgacggtgg cgacatcgcc cagccttacc
    31801 tctgagccgt cctgattcac ccgcaaggtg atatcgcgga actgttctgg tgtttgcagc
    31861 agtgactggg cgttaatggt ggcgttgagc gcctgcttat cgacggaagg cgtaccacca
    31921 agttgcccaa ccgcaatctg cgcgttctgt gactcaatgg catcagtgac atctttagcc
    31981 gtcatctgga aactgttgag tttcgccggg tccagccaga tacgcatgga atattgcgaa
    32041 ccataggcat cgatatcccc gacgccattc acgcggctta acggatcctg aatattactg
    32101 gcaacgtaat cggcaatatc ctgtttatcc atcgaaccat cggtagagac gaaggcaatg
    32161 gtcagaatgt tggtatcgcc ggttttacgc accgtcacgc cctgattttg caccgcctgc
    32221 ggtaactttc gcatggctga ttgcagctgg ttttgtactt gctgcacggc ttcgtccgga
    32281 tcggtgcctg ctttaaaact taaagtgaca gatgcctggc cggtgccact gctctgagac
    32341 gacatataca tcagattatc gagaccggtc atattttgct cgataacctg ggtcacggtg
    32401 ttttccagcg tctgggccga tgcgccggga tagttagcgg tcactcgcac attcggtggc
    32461 gcgagatcgg ggtattgttc aacgggcaat gagaaaatcg ccagggtccc tgtcagacac
    32521 aacaggattg ccagcaccca ggcaaaaatg gggcgatcaa taaagaaatt cgccattaaa
    32581 agaggacctc gtgtttctgc gtatcgttgt aggcttgact tgcttcactg tagcggcaag
    32641 gtacgagcca atcgtggaga aaaaaaggag ttaatgtaaa ttatcatgcc cttttatact
    32701 gaaccttaag tgccactatt ctttggtcaa taagaggcat tacatcagct gactttcctc
    32761 accctccgca gagcgaaagc taatactcac taacgtgccg ccgccggaag gttgcgaaaa
    32821 agtcagcgtc ccacccaatc tttccgcgcg ttcgcgcatg atattcagac cataatggcc
    32881 ttcgggttct ttcggttcac cgatgccaat cccgttgtcg cgaatataga ccgtgtgatt
    32941 gccatccggc gcggtgacgc agctaacggc gatttcgctg gcgttggcgt gcttcatcgc
    33001 attcagcacc gcttcgcgaa taatttgcaa caaatgcacc tgcatttgcg catccagcgc
    33061 cagggttggc aggcggcagt cgagggtcag tttagcgctg gtttgatttt gtaacgtatc
    33121 cagcatttcc ctcaacgcgg aggggagatc tgcctgctgc agcgtcaggc ggaaagtagt
    33181 caacagctcg cgtaactgcc gataagcatc attcaacgcc tgggaaaaat cggccatgat
    33241 actttgtgcg gtggcgttat cttccggtat cgaacgcttc agtaacgtca actggatacg
    33301 taagtaagaa agtacctgag ccagcgagtc gtgcaattcg cgggcgatgg tcgcacgttc
    33361 ttccatcaac aataactgct gaaaatgctt ctgcgcctga ttaaagtaca aaccgcgtcc
    33421 cagcatcgac gaaacgctgt taagcagcgg ttctgatgat gaaacgtgac tattttgcca
    33481 gtgcagttcg ccgtaaaccg tctcttgcat tgtcactggt aaaatctgca tcggcaattc
    33541 cgggtttggt tgcccttcgc taatccgcca gttttcaccg acatttaact ccagatattc
    33601 agccgcttcg ttgtcgcgca caatctgcaa aatatggcgg aaacaatgca catcaatctg
    33661 gctggtgttc agcgcctgcg aacactgata caacacctcc aggcgacgct tggcctcgtg
    33721 gaggtcgcgg gtcttttctt ctactgacgc ctccagcgaa cggtacaatt tatgtagctc
    33781 gctcgacatc tggttaaagg ttttggcaag caggccaagc tcattcggca ggctggtatc
    33841 cagcggcggc gagtcgaact gtccgtgttc aatacgctga ctggcggtaa ccagctgatt
    33901 cagcggggca accacctgat ggcgtatgcg gcgcaaagta aaaaagacca gcgtgaaaat
    33961 accgatgccg ccagccaggg aaatcgccac caccagcagc attttgcgtt cagcgtagtg
    34021 ctgtaaagcc agcacgaaca ggtctatttg attaacataa ttattaatat tggcctgata
    34081 ccacggcaag tcgcccttgc tgagccgatt attcatctcc agccagttgg cattcagatg
    34141 cgcgtagcgg gtttttactg cctctggcac ataccagacg ttgagattgg ttaataccgg
    34201 tgaatgcagt gcctgctgaa acaactggcg atgtgcattg agttgtggac tgccactttg
    34261 caagtcgtag cccaggcggt aactctgcat acgcagcgat ccggcaatat tgatagcctc
    34321 agcgtcgcgt aaactgctcg ccagcgttag cagagcgata cccgtggaaa gaatcgacag
    34381 cagcacaatg taaaaaaagg cccgggccag actggccgag acgggtcgtt taacaatcac
    34441 acgcgtcttc tccacaaaaa atcattaatg ttccagttcg ctgaaacgca atttagtcag
    34501 tgatgggatt atttgatctg ccacagttta tagcgaaata gttacttatt ctgggcacat
    34561 gaacattgcc gattcccctg taagtcgggt aataacaaca ctcatataaa gcataaggtt
    34621 tttacaacca aaaaagaagg tcgttatgaa tcgttttatt atggccaaca gtcagcaatg
    34681 tctgggttgt catgcttgtg aaatcgcctg tgtcatggct cacaatgatg agcaacatgt
    34741 cctgagccaa caccattttc atccccgaat tacggttatc aaacatcagc agcaacgtag
    34801 tgcagtgacc tgtcaccatt gcgaagatgc gccctgcgcc cgtagttgcc ctaatggcgc
    34861 aatcagccac gttgatgaca gcattcaggt caatcagcaa aagtgtattg gctgtaaatc
    34921 ctgcgtggtg gcctgtccat ttggtacgat gcaaatcgtc ctgacacccg tcgcggcggg
    34981 caaagtaaaa gccacggcgc ataaatgcga cctttgtgca gggcgcgaaa acggtcctgc
    35041 ctgtgtcgag aattgcccgg cggacgcgct gcaactggtc actgatgccg cactctccgg
    35101 catggcgaaa tcccgccgcc tgcgcaccgc acgtcaggaa catcaaccgt ggcatgccag
    35161 taccgcggca caagaaatgc cggtaatgag taaagtcgag caaatgcagg caacgcccgc
    35221 gcgtggcgag ccggataaac tggcgattga agcgcgcaaa accggttttg atgaaattta
    35281 tctgccattc cgtgtcgatc aggcacaacg ggaagcctcg cgctgcctta aatgcggcga
    35341 gcacagcgtt tgtgaatgga cctgcccgct gcataaccat attccgcagt ggattgaact
    35401 ggtgaaagcc ggaaacatcg acgccgccgt cgagctttct caccagacca acaccctgcc
    35461 ggaaattacc ggacgcgttt gtccgcaaga ccgtttgtgt gaaggtgcct gtactattcg
    35521 cgatgagcac ggcgcagtaa ctatcggcaa cattgaacgc tacatttcag atcaggcgtt
    35581 ggcgaaaggt tggcgtcctg acttaagcca tgtcaccaaa gtggacaagc gggtggcgat
    35641 tatcggtgcg ggtccggcag ggctggcctg tgcggatgtt ctgacccgca atggcgtaga
    35701 ggtgacggtg tacgatcgcc atccagaaat cggtggcttg ctcactttcg gcattccttc
    35761 tttcaaactg gataaatccc tgctggcacg ccgtcgggaa atcttcagcg cgatggggat
    35821 tcacttcgaa ctcaattgtg aagtgggtaa agatgtctct ttggattcgc ttttggaaca
    35881 atacgacgcg gtcttcgttg gcgtaggcac ttaccgttcc atgaaagcgg gtttacccaa
    35941 tgaagatgcg ccgggcgttt atgacgcgct gccgttcctc attgccaaca ctaaacaggt
    36001 gatgggactc gaagagctac cggaagagcc gtttatcaat accgccggac tcaacgtcgt
    36061 ggtactgggc ggcggcgaca ccgcgatgga ctgtgtgcgt actgcgctgc gccacggcgc
    36121 gagtaacgtc acctgcgctt atcgtcgtga tgaagctaac atgccaggct cgaagaaaga
    36181 ggtgaagaac gcccgcgaag agggggccaa cttcgaattt aacgtccagc cggtggcgct
    36241 tgagctgaat gaacaaggtc acgtttgcgg gattcgtttc ctgcgtacgc gtcttggcga
    36301 gccggatgcc caggggcgtc ggcgtccagt accggtggaa ggcagtgaat ttgtcatgcc
    36361 agccgacgcg gtgattatgg cgtttggctt caatccgcac gggatgccgt ggctggagtc
    36421 gcacggtgta atggtagaca aatggggccg catcatcgcg gatgtggaaa gccagtaccg
    36481 ttaccagacc accaatccga aaatcttcgc tggtggtgac gccgtgcgtg gtgcggatct
    36541 ggtggttacc gcaatggcag aaggacgtca tgcggcacag gggattattg actggctggg
    36601 ggtaaaatca gtcaaatctc actgatagcc tgcgcagaca aacccgactt cacagcgtaa
    36661 gataattgtt catttcgcgc tgtggagtcg gtatgacgca acaaatcacc ctcattaaag
    36721 acaaaattct ctccgataac tatttcaccc tgcacaacat tacctacgat ctcacgcgca
    36781 aagatggcga agttatccgc cataaacgtg aagtttacga tcgcggtaat ggtgcgacga
    36841 tcctcctgta caacgcgaag aaaaagaccg tggttctgat tcgtcagttc cgcgtcgcta
    36901 cctgggttaa tggcaatgaa agcgggcagc tgattgaaac ctgcgccggg ctgctggata
    36961 acgacgaacc ggaagtgtgt attcgtaaag aagcgattga agagacgggt tatgaagtgg
    37021 gcgaagtgcg caaattattt gaactgtata tgtcgccagg cggtgtgact gagctaatcc
    37081 acttctttat cgccgaatac agtgacagcc agcgcgctaa cgccggtggt ggtgtcgaag
    37141 atgaagatat tgaagtgctc gagctgccgt tcaacaaggc gctggagatg atcaaaactg
    37201 gcgagatacg tgacggtaag acggtgttat tgcttaacta tttgcaaatg tcacatttaa
    37261 tggactgaaa aataacaata atatttcgtt gtttattatt ggctcaatcc gataaatccg
    37321 attgagccgc gctacctgcg caacgaagat acgacttgtg ctgtttgttt gaacttctgg
    37381 ggtcgtaccg tccatgcgct atcgcatttt ccttctcttt tttttcgctt tgttgccgac
    37441 gtctttggtg tgggcggcac cagcgcaacg ggcgttttcc gactggcagg tcacctgtaa
    37501 taaccaaaac ttctgcgtgg cgcgtaatac gggcgatcat aacggactgg tgatgaccct
    37561 gagccgcagc gccggggcgc atacggatgc cgttttacgt attgagcgcg gcgggttgaa
    37621 gtcgccggat gcgtctgaag gggagatagc gccacggctg ctgttagatg gcgagccgtt
    37681 ggcgttaagt ggtgacaagt ggcggatttc accatggtta ttagtaaccg atgatacggc
    37741 aaccatcacc gcgtttttgc agatgattca ggaagggaag gcgatcacct tacgcgacgg
    37801 caatcagacc atttctctga gtggcttaaa agcggcgttg ttgtttatag atgctcagca
    37861 aaagcgcgtt ggcagtgaaa ccgcgtggat aaagaaaggg gatgaaccgc cgctcagcgt
    37921 accgcccgcg cctgcgctga aagaggtcgc ggtggttaac ccaacgccga cgccactctc
    37981 actcgaagaa cgcaacgatt tgctggatta tggaaactgg cggatgaatg gtctgcgctg
    38041 ctcgcttgat ccgttacgtc gtgaggtgaa tgtcactgcg ctgaccgatg ataaagcgct
    38101 gatgatgatt agctgtgagg caggggccta taacaccatt gatttggctt ggattgtgtc
    38161 gcgtaaaaag ccactggcct cgcgcccggt tcggttacgt ttgccgttca acagcggtca
    38221 ggagacgaat gaactggaac tgatgaacgc aacattcgat gaaaaatcgc gtgaactggt
    38281 gaccttagca aaagggcgcg gattaagcga ttgtggcatt caggcgcgct ggcgctttga
    38341 cggtcagcgt tttcgcctgg tgcgttatgc cgcagaaccc acctgcgata actggcatgg
    38401 gccagacgcc tggcccacgt tgtggatcac ccgttaaaca aaatcccaga tgcgccccta
    38461 aaaagcgcac ggactgtccc ctcgcccctc tggggagagg gttagggtga ggggaacata
    38521 cagcatccgg cgatcaccat caggcacctt tcactcccag caccttatgc gcttttgcca
    38581 caatattctc ggcggtaaag ccaaagaacg ggaacagctt atctgccgga gcagattccc
    38641 catagccggt catcccgaca attgcccctt tcagaccaac atatttgtac cagtaatcgg
    38701 cgatgcctgc ttccaccgcc acgcgagccg caacgttaga aggcaacacc gactcgcgat
    38761 attcctcatc ctgggcgtcg aaaatatcgg tcgagggcag ggaaactacg cgtacattgc
    38821 gaccttctcc cgctaatttc tctgccgctt gcagggtgat ttccatctct gaaccggtgg
    38881 caatcaaaat aatatctggc ttaccgccgc tgtctttcag tacataaccg ccacgagcaa
    38941 tctctttaac ctgatccggc gtacgttcca cctgggccag attctgcctg gagaggatca
    39001 gtgccgtcgg tccgttgtgg cgctcaacgg ccagcttcca gcccaccgct gcttccacct
    39061 gatcgcacgg tcgccaggtg ctgaaatttg gcgttaagcg cagactggcc agttgctcaa
    39121 cagcctggtg cgtaggacca tcttcgccca gaccaattga gtcgtgggta taaaccataa
    39181 tctgccgcgc tttcattagt gccgccatcc gcgcggcgtt acgggcgtat tcaacaaaca
    39241 tcaggaacgt cgcggtatac ggcacaaagc cgccgtggtg cgcgatgccg ttggcgatag
    39301 cggtcatgcc aaattcacgc accccgtagt gaatgtagtt gcccgccgga tcttccttca
    39361 gcgaaacaga acctttccag atggtcaggt tgctgggagc cagatccgcc gaaccgccga
    39421 gcaactcagg cagcatcggc ccgtaagcgt taagcgtatt ttgcgaagcc ttacgggtag
    39481 cgattttcgc cggatttgcc tgtaattcat tgatatattt ctgagtcgtt ttctcccagt
    39541 ccttcggcaa accgccgctc atccgacggg taaactcttc tgccagttgc ggatgggcct
    39601 ttttataggc ggcaaacttc tcattccagc tctgctgcgc tttttcgcct ttttcgcggg
    39661 catcccaggc gtgataaatc tctttaggga tctcaaatgg cggatggtgc cagcccagtt
    39721 tttgccgtgc cagcgccact tcttcttccc ccagcggtgc gccgtgcgcc tcttccttac
    39781 ctgctttatt cggcgaacca aagccaatta ctgtgcggca gataatcagc gacggcttat
    39841 ctttcacgct ttgcgcttca aggatcgctt tcttcaccgc ctgcggatcg tgaccgtcga
    39901 tttcatggat cacatgccag tgataggctt caaaacgttt tgctgtatcg tcggtaaacc
    39961 agccttcggt ttcaccgtcg atggaaatac cgttgtgatc gtaaaaacca atcagcttgc
    40021 ccagtcccag cgtgcccgcc agcgaacaga cttcgtggga aataccttcc atcaggcaac
    40081 cgtcgcccat aaacacatag gtgaagtgat cgacaatttc atgatccggc tggttaaact
    40141 gcgccgccag tgtacgcgct gctatcgcca gtccgacggc gttcgccaaa ccttgtccaa
    40201 gtggaccggt ggtggtttca acacctggcg tatagccaat ctccgggtga cctggggttt
    40261 ttgaatgcaa ctgacggaag ttcttcagct cttccagcgg caggtcgtaa ccggtcagat
    40321 gtagcaaact gtagagcagc atcgacgcgt ggccgttgga aagaataaag cggtcgcgat
    40381 cataccaggt tgggtcggtg gggttatgtt taagaaaatc gttccacagc acttcggcaa
    40441 tatcagccat gcccatcggc gcgccgggat gaccagaatt ggctttttgt accgcatcca
    40501 tactgagtgc gcgaatcgca ttggcaaggt cttttcggga catataacac tccgtggctg
    40561 gtttatagtt tggcggcaag aagatcttcc agtttgcgtt gatcaacggc gaacagacga
    40621 atgccttcag acagtttttc taccgccatc gcatcctgat tgtgctccca acggaactcc
    40681 gcttcgctca tgggagcggg gcgtgggaac gtctgagaag gtgggattaa tttacgtacc
    40741 actggcgaaa ctttttcctg cagctccttc agcaaattcg gtgcgatagt cagtcgatcg
    40801 cagccggtta aggcgaggat ttgttcggtg cgacggaagc tcgcgcccat cacaatggtt
    40861 tcatagtggt gttgcttata gtagtcgtag atattgcgta ctgatttaac gcctggatct
    40921 tcttccacca catacgggtc catcggcttg cgtgcctgat accagtcata aatacgcccg
    40981 acaaacggcg aaatcagaaa aacgcctgct tctgcacagg cccgcgcctg tgcaaaagaa
    41041 aacagcagcg tcaggttgca gttaatacct tctttttcca gctcttctgc cgcgcgaatt
    41101 ccttcccagg tcgaagccag tttgatcaga atgcgtgatt tctcaacgcc ttgttgctga
    41161 tacaagtcca ccagatggcg cgccttctca atactctttt ctttatcaaa agagaggcgt
    41221 gcatcaactt ctgttgacac gcgaccgggt acgattttga gaatttcagc accgaaattg
    41281 accgccagtt tgtcacacgc tgcgaccacc tgttgttcct gggttttgcc attttttcta
    41341 ccccaggcga tagcatcgtc tattaaatgc tcatattgtg ataatccggc agccttgagt
    41401 aacagcgaag gattggtggt ggcatcctgg ggatgataat ggcgaatgga ctcaatatcg
    41461 ccgctgtctg ccacgacagt ggtgaactgt ttgatgccgt ctaactcgtt cataggaaat
    41521 actccttgaa aagtaaagtg ttagatgagt gcgttaattc acacttctga gaaatttcgc
    41581 taaacgcatc aaaaaagcat agcagacagg catggtattg ctggattaag caggtaacat
    41641 cagtgttata ggattattac caaaacatta tattaatggt gatagtttgg acttgctcaa
    41701 agtctgtaga ctccggcagg gtaataatgt gcgccacgtt gtgggcaggg gctgttgccc
    41761 acacacttta tttgtgaacg ttacgtgaaa ggaacaacca aatggatgac cagttaaaac
    41821 aaagtgcact tgatttccat gaatttccag ttccagggaa aatccaggtt tctccaacca
    41881 agcctctggc aacgcagcgc gatctggcgc tggcctactc accaggcgtt gccgcacctt
    41941 gtcttgaaat cgaaaaagac ccgttaaaag cctacaaata taccgcgcgc ggtaacctgg
    42001 tagcggtgat ctctaacggt acggcggtgc tggggttagg caacattggc gcgctggcag
    42061 gcaaaccggt gatggaaggc aagggcgttc tgtttaagaa attcgccggg attgatgtat
    42121 ttgacattga agttgatgaa ctcgacccgg acaaatttat tgaagtcgtc gccgcgcttg
    42181 aaccgacctt cggcggcatc aacctcgaag acattaaagc gccagaatgt ttctatattg
    42241 aacagaaact gcgcgagcgg atgaatattc cggtattcca cgacgatcag cacggcacgg
    42301 caatcatcag cactgccgcc atcctcaacg gtttgcgcgt ggtggagaaa aacatctctg
    42361 acgtgcggat ggtggtttcc ggcgcgggtg ccgcagcaat cgcctgtatg aacctgctgg
    42421 tagcgctggg tctgcaaaaa cataacatcg tggtttgcga ctcaaaaggc gttatctatc
    42481 agggccgtga accaaacatg gcggaaacca aagccgcata tgcggtggtg gatgacggca
    42541 aacgtaccct cgatgatgtg attgaaggcg cggatatttt cctgggatgt tccggcccga
    42601 aagtgctgac ccaggaaatg gtgaagaaaa tggcccgtgc gccaatgatc ctggcgctgg
    42661 cgaacccgga accggaaatt ctgccgccgc tggcgaaaga agtgcgtccg gatgccatca
    42721 tctgtaccgg ccgttccgac tacccgaacc aggtgaacaa cgttctttgc ttcccgttca
    42781 tcttccgtgg cgcgctggac gttggcgcaa ccgccatcaa cgaagagatg aaactggcgg
    42841 cggtacgcgc gattgcagaa ctggcccatg cggaacagag tgaagtggtg gcttcagctt
    42901 atggcgatca ggatctgagc tttggtccgg aatacatcat tccaaaaccg tttgatccgc
    42961 gcttgatcgt taagatcgct cctgcggtcg cgaaagcagc gatggagtcg ggcgttgcga
    43021 cgcgtccgat tgctgatttc gacgtctaca tcgacaagct gactgagttc gtttacaaaa
    43081 ccaacctgtt tatgaagccg attttttccc aggctcgcaa agcgccgaag cgcgtcgttc
    43141 tgccggaagg tgaagaggcg cgcgttctgc atgccactca ggaactggta acgctggggc
    43201 tggcgaaacc gatccttatc ggtcgtccga acgtgatcga aatgcgcatt cagaaactgg
    43261 gcttgcagat caaagcgggt gttgattttg agatcgtcaa taacgaatcc gatccgcgct
    43321 ttaaagagta ctggactgaa tacttccaga tcatgaagcg tcgcggtgtc actcaggagc
    43381 aggcgcagcg tgcgctgatc agtaatccga cagtgattgg cgcgatcatg gttcagcgtg
    43441 gcgaagccga tgcaatgatc tgcggtacgg tgggcgatta tcatgaacat tttagcgtgg
    43501 tgaaaaatgt ctttggttat cgcgatggcg ttcacaccgc aggtgccatg aacgcgctgc
    43561 tgctgccgag tggtaacacc tttattgccg atacatacgt taatgacgaa ccagacgcag
    43621 aagagctggc ggagatcacc ttgatggcgg cagaaactgt ccgtcgtttc ggtattgaac
    43681 cgcgcgttgc tctattgtcg cactccaact ttggttcttc tgactgcccg tcttcgagca
    43741 aaatgcgtca ggcgctggaa ctggtcaggg aacgtgcacc agaactgatg attgatggtg
    43801 aaatgcacgg cgatgcagcg ctggtggaag cgattcgcag cgaccgtatg ccggacagcc
    43861 ctttgaaagg ttccgctaat attctggtga tgccgaacat ggaagctgcc cgcattagtt
    43921 acaacttatt gcgcgtttcc agctcggaag gtgtgactgt cggcccggtg ctgatggggg
    43981 tggcgaaacc ggttcacgtg ttaacgccga tcgcatcggt acgtcgtatc gtcaacatgg
    44041 tggcgctggc tgtggttgaa gcgcaaaccc aaccgctgta attcattctt aactctcacg
    44101 cttaccctga atattcaggg taagcggttt tcctgaaata tattagtaaa gcttattact
    44161 gagtttacga ataataaaaa aagcagtata tgaaatatct cgatattatt ttatttatat
    44221 tcatgcgtcg catatgaaag tttattcata acagtaaata tctctccatt cttagtgatc
    44281 tacctcacct tttaaacgcg cttgccgaat tttgttattt actctgacga aaaattgtca
    44341 cgatacacga aagtttttca caggcggcga ctcatggata aagaacgcat cattcaggaa
    44401 tttgtgcccg gtaaacaggt cacactggcg catctcatcg cgcatcccgg cgaggaactg
    44461 gcgaagaaaa tcggcgttcc tgatgcgggc gcaatcggca ttatgacgct aactcccggc
    44521 gaaacggcga tgatcgccgg tgatttagca ctaaaagccg ccgatgtgca tatcggtttt
    44581 ctcgaccgtt ttagcggcgc gttggtgatt tatggctcgg tgggtgcagt agaagaagcg
    44641 ttatcacaaa ccgtcagcgg tttagggcgt ttattaaatt atacgctttg tgaaatgacc
    44701 aaaagttaat acgaggcatt catgaaacga attgcgtttg tgggtaccgt cggggcagga
    44761 aaaacaacgt tatttaatgc gttgcaggga gattataccc tcgccagaaa aacgcaggcc
    44821 gtagaattta atgataaggg tgatattgat acgccgggtg aatattttag ccatccccgc
    44881 tggtatcacg ccttaattac cacgctacag gatgtggaca tgctgatcta tgtccacggc
    44941 gcgaatgacc cggaaagtcg gttgcctgcc gggttgctgg atattggcgt cagcaaacgg
    45001 caaatcgccg tcatcagtaa aacggacatg ccagatgctg acgtcgccgc aacgcgaaag
    45061 ttattgcttg agacgggctt cgaagagccg attttcgagc taaacagcca cgacccgcaa
    45121 agcgtgcagc aactggtgga ttatctggca tctctcacca aacaggagga agcaggtgaa
    45181 aaaactcatc acagcgaatg atattcgtga agcacacgcg cgcggcgaac aggcaatgtc
    45241 cgtggttctg cgcgccagca ttattacccc ggaagcccgt gaagtggcag atctactggg
    45301 ttttaccatc actgaatgtg atgaatcgac cccggtaacg gcgtccgttc ccgcctgcgt
    45361 gccagccgat aaaaccgaaa gccagcgtat ccgcgaaacc atcatcgccc agctaccgga
    45421 aggccagttt accgaaagcc tggtcgcgca gctgatggaa aaagtgatga aggaaaaaca
    45481 gtcactggag cagggtgcaa tgcagccgag tttcaaatcg gtgaccggca aaggcggcat
    45541 caaagttatt gatggcagca gcgtcaagtt tggtcgtttc gacggcgcag agccgcattg
    45601 cgtgggcttg actgatcttg tgaccggaga cgacggcagc agcatggccg ccggttttat
    45661 gcagtgggag aacgcattct tcccgtggac cctgaactac gacgaaatcg acatggtgct
    45721 ggaaggggag ctgcatgttc gccacgaagg cgaaaccatg atcgccaaag caggcgacgt
    45781 aatgtttatc ccgaaaggct ccagcatcga atttggtacg acatccagcg tgaaattcct
    45841 gtatgtcgcc tggccggcta actggcaatc cctatgaaag atttcatcac cgaagcatgg
    45901 ctaagagcga atcatacgct tagcgaaggc gcagagatcc atctccctgc ggacagtcgc
    45961 ctgacgccct ctgcccggga gttgctggaa agccgccatc tgcgcatcaa gtttattgac
    46021 gagcagggcc gcctgtttgt tgacgatgaa cagcagcagc cgcagcccgt tcacgggctg
    46081 accagtagtg atgaacatcg gcaggcgtgc tgcgaactgt gccgccagcc ggtggcgaaa
    46141 aagccggaca cgctgactca cctgtcggcg gaaaaaatgg tcgccaaaag cgatccgcgt
    46201 ctgggttttc gcgcggttct cgacagcacc attgcgctgg cagtgtggct gcaaattgaa
    46261 ctggcggaac cgtggcagcc gtggctggcg gacatccgct cgcgtcttgg caacattatg
    46321 cgcgccgatg cgctggggga accgctggcc tgccaagcga ttgtcgggct ttctgacgaa
    46381 gatctgcatc ggctttctca tcagccgctg cgctatcttg gtcacgatca tctggtgccg
    46441 gaagccagcc acgggcgcga tgcggcgtta ctcaatctgc tacgcaccaa agtgcgggaa
    46501 accgaaaccg tagcggcgca ggtgtttata acccgcagtt ttgaagtatt acgcccggac
    46561 atcttgcagg cgctgaaccg cctttcgagc acggtctacg tgatgatgat cctgagcgta
    46621 acgaagcagc cgctaacggt gaagcagatc caacaacgac tgggggaaac gcaatgatta
    46681 ttgaacgttg tcgtgaactg gcgttgcgag cgcccgccag agtggttttt ccggatgcgt
    46741 tagatcaacg tgtgctgaaa gccgcgcaat atttacatca acaaggtctg gcaacgccca
    46801 ttctggtcgc caatccgttt gaacttcgtc agtttgcgct cagtcacggc gtggcgatgg
    46861 acgggctaca ggtgatagat ccgcatggca acctcgcaat gcgggaagaa tttgctctcc
    46921 gctggctggc ccgtgcgggc gaaaaaacgc cgccggatgc gttggaaaaa ctcaccgatc
    46981 cgctgatgtt cgctgccgca atggtcagcg ccgggacagc ggatgtctgt atcgcgggca
    47041 atctctcttc cacggcgaat gtgctgcgtg ccggattacg cattattggc ttacagccag
    47101 gctgtaaaac gctctcctcc attttcctga tgctgccaca gtacagtggc cccgcattgg
    47161 gctttgccga ttgcagcgta gtgccacagc cgacggcggc gcaactggcg gatatcgcgc
    47221 ttgccagcgc cgaaacctgg cgcgccatca ccggagaaga accgcgcgtg gcgatgctgt
    47281 cgttttccag caacggtagt gcccgtcacc cctgcgttgc caacgtccag caggcgacag
    47341 aaatcgtccg tgagcgcgca ccgaagctgg tggtggatgg cgagttgcag tttgacgccg
    47401 ccttcgtgcc ggaagtggcg gcgcaaaaag cgcctgccag cccgctacaa ggcaaggcca
    47461 atgtgatggt ttttccgtcg ctggaagccg gaaatattgg ctacaaaatc gcacaacgac
    47521 tcggcggata tcgtgccgtc gggccattga tacaagggct tgccgcgccg atgcacgatc
    47581 tctctcgtgg ttgtagcgtg caggaaatta tcgagctggc gctggtggca gctgtgccgc
    47641 gtcagacaga agtgaaccgc gaaagcagtt tacaaacact ggttgaatga acggtcccgt
    47701 tctggaccct gataaagagg aaagcacaat ggaagcatta ggaatgatcg aaacccgggg
    47761 cctggttgca ctgattgagg cttctgacgc gatggtgaaa gctgcgcgcg tcaagctggt
    47821 tggtgttaag cagattggcg gcggcctgtg tactgccatg gttcgtggtg atgttgcagc
    47881 ctgtaaagcc gcgaccgacg caggtgccgc cgcggcacag cgtatcggcg agctggtatc
    47941 tgtacacgtt atcccgcgtc cgcacggcga cctggaagaa gtcttcccga tcggcctgaa
    48001 aggcgatagc agcaacctgt aatcaccctg tgtgacgtcg attcgggcgt cacgctctct
    48061 cctttttcag tttaccttcc gcggagggta gggggagctt ttacctgaaa tacgccacgg
    48121 aggcgggtat gaaactggca gtcgtcactg gacaaattgt ttgtaccgta cgccatcacg
    48181 gactggcgca tgacaaattg ctgatggtgg aaatgattga tccacaaggt aatcctgacg
    48241 ggcaatgcgc cgtcgccatc gacaatattg gcgcgggaac cggggagtgg gtgttgctgg
    48301 tgagtggcag ttccgcccgc caggcgcata aaagcgaaac gtcaccggtc gatctgtgcg
    48361 tgattggcat tgtcgatgag gtggtgtctg gcggtcaggt aattttccac aaataaggca
    48421 gaacatcatg aatcaacagg atattgaaca ggtggtgaaa gcggtactgc tgaaaatgca
    48481 aagcagtgat acgccgcccg ccgccgttca tgagatgggc gttttcgcgt ccctggatga
    48541 cgccgttgcg gcagccaaaa ttgcccagca agggttaaag agcgtggcaa tgcgccagtt
    48601 agccattgct gccattcgtg aagcaggcga aaaacacgcc agagatttag cggagcttgc
    48661 cgtcagtgaa accggcatgg gccgcgtcga agataaattt gccaaaaacg ttgctcaggc
    48721 gcgcggcaca ccgggcgttg agtgcctctc tccgcaggtg ctgactggcg acaacggcct
    48781 gactttaatt gaaaacgcgc cctggggcgt ggtggcttcg gtaactccgt ccaccaaccc
    48841 ggcggcaacc gtaattaata acgccataag cctgattgcc gcgggcaaca gcgtcatttt
    48901 tgccccgcat ccggcggcga aaaaagtctc ccagcgggcg attacgctgc tcaaccaggc
    48961 gattgttgcc gcaggcgggc cggaaaactt actggttact gtggcaaatc cggatatcga
    49021 aaccgctcaa cgtttgttca agtttccggg catcggcctg ctggtggtga ccggcggcga
    49081 agcggtagta gaagcggcgc gtaaacacac caataaacgt ctgattgccg caggcgctgg
    49141 caacccgccg gtagtggtgg atgaaaccgc tgacctcgcc cgtgctgctc agtccatcgt
    49201 caaaggcgcg tctttcgata acaacatcat ttgtgccgac gaaaaggtac tgattgttgt
    49261 ggatagcgta gccgatgaac tgatgcgtct gatggaaggc cagcacgcgg tgaaactgac
    49321 cgcagagcag gcgcagcagc tgcaaccggt gttgctgaaa aatatcgacg agcgcggaaa
    49381 aggcaccgtc agccgtgact gggttggtcg cgatgcggcg aaaatcgcgg cggcaatcgg
    49441 ccttaaagtt ccggaacaaa cgcgactgct gtttgtagaa accaccgcag aacatccgtt
    49501 tgctgtgact gaactgatga tgccggtgct gcccgtcgtg cgcgtcgcca acgtggcgga
    49561 tgccattgcg ctggcggtga aactggaagg cggttgccat cacacggcgg caatgcactc
    49621 gcgcaacatc gaaaacatga accagatggc gaatgccatt gataccagca ttttcgttaa
    49681 gaacggaccg tgtattgccg ggctggggct gggcggggaa ggctggacca ccatgaccat
    49741 caccacgcca accggtgaag gggtaaccag cgcgcggacg tttgtccgtc tgcgtcgctg
    49801 tgtattagtc gatgcgtttc gcattgttta aggagtaaca gatggcgcac gacgaacaat
    49861 ggctcacccc acgtctgcac atagcagcaa cgctgtgtaa ccagacgcct gccgcgacgg
    49921 aatctccgct gtggctgggg gtggatctgg gcacctgcga tgtggtgtcg atggttgttg
    49981 accgcgacgg tcagccggtg gcggtatgcc tcgactgggc tgatgtggtc cgcgacggca
    50041 tcgtctggga tttcttcggt gcggtcacca ttgttcgtcg ccatctcgac accctcgagc
    50101 agcaattcgg tcgccgtttt agccatgcgg cgacctcttt tcctccaggt acggacccgc
    50161 gtatttccat caacgtgctg gagtctgccg ggctggaagt gagccatgtg ctggatgagc
    50221 caaccgcagt cgcggatctg ctgcaactgg acaacgccgg tgtagtggat atcggcggcg
    50281 gcaccaccgg cattgccatc gtgaaaaagg gcaaggtgac ttactcggca gatgaagcca
    50341 ccggcggaca tcatatctct ctgactctcg ccggaaatcg ccgtatttcg ctggaagagg
    50401 cggagcagta caagcgcggt cacggtgaag agatttggcc cgcggtgaaa ccggtgtacg
    50461 aaaaaatggc ggacattgtc gcccggcata ttgaagggca gggcattact gatttatggc
    50521 tggcgggcgg ctcctgtatg caaccgggcg ttgcggaact gttttgcaaa caattcccgg
    50581 cgttacaggt gcatttaccg cagcacagtt tgtttatgac cccgctggcg atcgccagta
    50641 gcgggcgaga gaaagcggag gggatctatg caaaatgaat tgcagaccgc gctctttcag
    50701 gcgttcgata ccctgaatct gcaacgggta aaaacattta gcgttccacc ggtgacgctt
    50761 tgcggtccgg gcgcgatgag cagttgcggg cagcaagcgc aaacgcgtgg gctgaaacat
    50821 ctgttcgtga tggcagacag ctttttgcat caggcgggga tgaccgccgg gctgacgcgt
    50881 agcctggccg ttaaaggtat cgccatgacg ctctggccat gtccggtggg cgaaccgtgc
    50941 atcaccgacg tgtgtgcagc cgtggcgcag ttgcgtgaat caggttgtga cggggtgatc
    51001 gcgtttggcg gcggctcggt gctggatgcg gcgaaagccg tggcgttgct ggtgacaaac
    51061 ccggatagca cgctggcaga aatgtcagaa accagcgttc tgcaaccgcg cctgccgttg
    51121 attgccattc caacgaccgc cggaaccggt tctgaaacca ccaacgtaac ggtgattatc
    51181 gacgcggtga gcgggcgcaa gcaggtgtta gcccacgcct cgctgatgcc ggatgtggcg
    51241 attctcgacg ctgcgctgac cgaaggcgtg ccgtcgcatg tcacggcgat gaccggtatc
    51301 gacgcgttaa cccatgctat tgaagcgtac agcgcgctga acgccacacc gtttaccgac
    51361 agtctggcga ttggcgcaat cgcgatgatt ggtaaatcac tgccgaaagc ggtgggctac
    51421 ggtcatgacc ttgccgcgcg cgagagcatg ttgctggctt catgcatggc gggaatggcg
    51481 ttttccagtg cggggcttgg gttatgccac gcgatggcgc atcagccggg ggcggcgctg
    51541 catattccgc acggtctggc gaacgccatg ttgttgccaa cggtgatgga atttaaccgg
    51601 atggtttgtc gtgaacgctt tagtcagatt ggtcgggcgc tgcgaactaa aaaatccgac
    51661 gatcgtgacg ctattaacgc ggtaagtgag ctgattgcgg aagtcgggat tggtaaacga
    51721 ctgggcgata ttggtgcgac atctgcgcat tacggtgcat gggcgcaggc cgcgctggaa
    51781 gatatttgtc tgcgcagcaa cccgcgtacc gccagcctgg agcagattgt cggcctgtac
    51841 gcagcggcgc aataaaagtg tacggacagt cccctcgccc ctttggggag agggttaggg
    51901 tgaggggaac agagtcgcgc cggtgcaaac aattaccctc accccggccc ctcggcttcc
    51961 agagtgaggg aaaaacccac tttcagcatt ggcaataaat aacaactaac agggagtaaa
    52021 ggcgatggga attaacgaaa tcatcatgta catcatgatg ttctttatgc tgatagctgc
    52081 cgtagacagg atcctgtcgc agttcggcgg ttctgctcgt ttcctcggta agttcggtaa
    52141 aagtatcgaa ggatccggcg ggcagttcga agaaggcttt atggcaatgg gcgcactggg
    52201 cctggcgatg gttggtatga ccgcgctggc acctgttctg gcccacgtac tcggaccggt
    52261 gattatcccg gtttacgaaa tgctcggcgc taacccatcg atgttcgccg gaacactgct
    52321 ggcgtgcgat atgggcggct tcttcctcgc caaagagctg gcgggcggcg acgtagcagc
    52381 gtggctatac tctgggttaa ttctcgggtc gatgatgggg ccaacgattg tgttttccat
    52441 tccggtggcg ctcggcatta tcgaaccttc tgaccgtcgt tatctggcgc tcggcgtgct
    52501 ggcgggcatt gtgaccattc cgattggctg tattgctggt ggtctggttg ctatgtactc
    52561 cggtgtgcag atcaacggtc agccggtgga attcaccttt gcgctgatcc tgatgaacat
    52621 gatcccggta cttatcgttg cggtgctggt ggcgctgggg ctgaaattca tcccggaaaa
    52681 aatgatcaac ggcttccaga tcttcgccaa attcctcgtt gcattgatca ccctcggtct
    52741 tgctgccgcg gtagtgaaat tcctccttgg ctgggaactg attcccggtc tggatcctat
    52801 ctttatggcc cctggcgaca aacccggtga agtgatgcgc gccattgaag ttatcggttc
    52861 tatctcctgc gttctgttag gggcgtaccc gatggtactg ctgctgaccc gctggtttga
    52921 aaaaccgctg atgagcgtcg gtaaagtgct gaatatgaac aacatcgcgg cagccggcat
    52981 ggtggcaacg cttgccaaca acatcccgat gtttggcatg atgaagcaga tggatacccg
    53041 cggcaaagtg atcaactgcg ccttcgccgt ttccgctgct ttcgccctgg gcgaccactt
    53101 aggcttcgcc gctgccaaca tgaacgccat gattttcccg atgattgtcg gcaaattgat
    53161 cggcggcgta acggcgattg gcgtggcgat gatgctggtg ccaaaagaag acgcgaccgc
    53221 ggctaaaacc gaagcggagg cacaatcgtg aacactcgcc agctactgag cgtcggtatc
    53281 gatatcggca ccaccaccac ccaggtgatc ttctcccacc tggagctggt taaccgtgcg
    53341 gcggtgtcgc aggtgccgcg ctacgaattc atcaaacgcg aaattagctg gcaaagcccg
    53401 gtgttcttta cccctgtcga taaacagggt ggtttaaaag aagcggaact gaaaacctta
    53461 atcctcgagc aatatcaggc tgcgggcatt gcgccggaaa gcgttgattc tggtgccatc
    53521 atcatcaccg gtgaaagcgc gaaaacccgc aacgctcgcc cggcggtgat gacgctctcc
    53581 cagtcgctgg gcgattttgt cgtcgccagc gccgggccgc atctcgaatc cgtgatcgcc
    53641 ggtcacggtg ccggggcgca aaccctttct gaacaacggc tgtgtcgggt actgaatatc
    53701 gacatcggcg gtggcaccgc gaactacgcc ctgttcgatg ccggaaaaat cagcggcact
    53761 gcctgcctca acgtcggtgg tcgcctgctg gaaaccgaca gccaggggcg cgtggtttac
    53821 gctcataaac cggggcagat gattgtggat gagtgcttcg gcgcaggcac cgacgcccgt
    53881 tcgctgaccg gcgcgcagct ggtgcaggtt acccggcgga tggcagagct gattgtcgaa
    53941 gtgattgacg gaacgctctc gccgctcgcg caggcattga tgcaaaccgg tttgctgccc
    54001 gcaggtgtta cgcccgaaat cattacgctt tctggcggcg tgggcgaatg ttatcgccac
    54061 cagcccgccg acccgttctg ttttgccgat attggcccgc ttctggcaac ggcgctgcat
    54121 gaccatccgc gcctgcgtga gatgaacgtg cagtttccgg cgcaaaccgt acgcgccacg
    54181 gtgattggcg cgggtgcaca taccctttcg ctctctggca gcacaatctg gctggagggc
    54241 gtacaactgc cgctgcgcaa tttgccggtg gcgatcccaa ttgatgaaac ggatctggtg
    54301 agtgcctggc aacaggcgct gattcagctg gatcttgatc ccaattctga cgcgtacgtg
    54361 ctggcgcttc ccgcctcgct gcctgtgcgt tacgccgcgg tactgacggt catcaacgcg
    54421 ctggtcgatt tcgtcgcgcg ttttccgaat ccgcatcccc tgctggtggt ggccgggcag
    54481 gactttggta aagctctggg catgttgttg cgcccacagc tacaacaact cccgttggca
    54541 gtcattgacg aagtgattgt ccgcgcgggg gactatatcg acattggtac gcctcttttt
    54601 ggcggatcgg ttgtgccggt gacggtgaaa tcactcgcat ttccttcctg agggaacgac
    54661 ttatgaaact aaagaccaca ttgttcggca atgtatatca gtttaaggat gtaaaagagg
    54721 tgctggctaa agccaacgaa ctgcgttcgg gggatgtgct ggcgggcgtt gcagcggcaa
    54781 gctcacagga gcgcgtggcg gcaaagcagg tgttgtcgga aatgaccgta gcggacatcc
    54841 gcaataatcc ggtgattgcc tatgaagatg actgtgtgac gcggctgatt caggacgatg
    54901 ttaacgaaac ggcctacaac cagattaaaa actggagcat cagcgaactg cgtgagtatg
    54961 tgctgagcga tgaaaccagc gtggacgaca ttgcctttac ccgcaaaggg ttgacctcgg
    55021 aagtggtcgc ggcggtggcg aagatttgct ccaacgcgga cctgatctac ggcgcgaaga
    55081 aaatgccggt aatcaaaaag gccaatacca ccatcggtat tccgggcacc ttcagcgccc
    55141 gtttgcagcc aaatgacacc cgtgacgacg tgcaaagtat cgctgcgcaa atctacgaag
    55201 ggctttcctt cggggtgggc gatgcggtga tcggcgtcaa cccggtgacg gacgacgtgg
    55261 aaaacttaag ccgcgtgctg gacaccatct acggcgtgat cgacaaattc aacatcccaa
    55321 ctcagggctg cgtgctggcg cacgtcacca cccagattga agcgatccgt cgtggcgcac
    55381 caggcgggct gattttccag agtatctgcg gcagcgaaaa agggctgaaa gaatttggcg
    55441 tggagctggc gatgctcgac gaagcgcgcg cagtgggcgc agagttcaat cgtatcgccg
    55501 gggaaaactg cctctacttc gaaaccggac aaggttcggc gctgtctgct ggcgctaact
    55561 tcggcgcaga ccaggtgacg atggaagcgc gtaactacgg gctggcgcgt cattacgatc
    55621 cgtttatcgt caacaccgtg gtcggcttta ttgggccgga gtatctctac aacgaccgcc
    55681 agattatccg tgcgggctta gaagatcact ttatgggtaa gctgagcggc atctctatgg
    55741 gctgtgactg ctgctacacc aaccacgctg acgctgacca gaacctcaac gaaaacctca
    55801 tgatcctgct cgccaccgca ggctgcaact acatcatggg gatgccgctg ggcgatgaca
    55861 tcatgctcaa ctatcagacc acagcattcc acgacactgc cactgtgcgt cagttactca
    55921 acctgcgccc gtcaccggag tttgaacgct ggctggaaag catgggcatt atggcaaacg
    55981 gtcgcctgac caaatgggcg ggcgatccgt cactgttctt ctgatgacgc ggagataaca
    56041 catcatggat caaaaacaga ttgaagaaat tgtacgcagc gtgatggcgt caatgggaca
    56101 agcggccccc gcgccgtcag aagcaaagtg cgccaccacc acctgtacga caccagtgac
    56161 ctcggaaagc tgcgcgctgg atttaggctc tgcggaagca aaagcgtgga ttggtgtgga
    56221 aaacccacat cgtgcggatg tgttaacaga actgcgtcgc agcaccgtgg cccgcgtttg
    56281 taccggtcgt gccggtccgc gtccgcgtac ccaggcgttg ctgcgtttcc tggccgatca
    56341 ctctcgttcg aaagataccg tactgaaaga agtgccggaa gagtgggtga aagcgcaggg
    56401 cttgctggaa gtccgctctg aaattagcga caaaaatctt tacctgactc gtccggatat
    56461 gggccgccgc ctgtgtgcag aagctgttga agcgctgaaa gcacagtgtg ttgctaatcc
    56521 ggacgtacag gtcgttattt ctgatggcct gtcaactgat gcgatcaccg tgaactacga
    56581 agagatcctg ccaccgctga tggcgggcct gaaacaggcc gggctgaaag tcggtacgcc
    56641 attctttgtt cgttatggtc gcgtgaagat tgaagatcag atcggtgaga tcctcggcgc
    56701 gaaagtagtg atcctactgg tgggggaacg cccgggatta gggcagtcag aaagcctctc
    56761 ctgctacgcc gtttactcgc cgcgtatggc aaccaccgtt gaggccgacc gtacctgtat
    56821 ctctaacatt caccagggcg gcacgccgcc ggttgaagcc gcagccgtca ttgtggattt
    56881 ggcgaaacgt atgctggagc agaaagcatc cggcatcaac atgacccgat aaggaggcat
    56941 catgccagct ttagatttga ttcgaccgtc ggtcaccgcc atgcgggtga ttgcctctgt
    57001 taacgccgaa tttgcgcgtg aactgaaatt gccgccgcat attcgtagcc ttgggctgat
    57061 ttctgctgat tccgatgacg tcacctacat tgcggctgac gaagcgacca agcaggcgat
    57121 ggtcgaagtg gtgtatggcc gctcgctgta tgcgggagct gcacacggac cgtcaccgac
    57181 tgccggtgaa gtgctgatta tgctcggtgg gccaaacccg gcggaagtgc gtgctggtct
    57241 ggatgcgatg gttgcgcata ttgaaaatgg cgcggctttc cagtgggcta atgacgcaga
    57301 aaacacagct ttcctggcac atgtagtttc gcgtaccggt tcttatctct catcaaccgc
    57361 cgggatcacg cttggcgatc cgatggcgta tctggtggca ccgccgctgg aagcgaccta
    57421 cggcattgat gcggcgttga aatctgccga cgtgcagctg gtgacctatg tcccgccgcc
    57481 gtctgaaacc aactactcgg cagcattttt aaccggtagc caggccgcgt gtaaagcagc
    57541 ctgtaacgct tttaccgatg cagtgctgga aatcgcgcgt aatccaatcc agcgtgcgta
    57601 acggaggttg ccgatgatca atgcactggg attgctggaa gtggacggca tggtcgccgc
    57661 gatagatgca gcggatgcca tgctcaaagc agctaacgtt cgtctgctca gtcacgaagt
    57721 gcttgaccct ggtcgcctaa cgctggtggt ggaaggcgat ctggcggcgt gtcgtgcggc
    57781 gctggacgca ggttgtgctg ccgcgatgcg taccgggcgt gtcatcagcc gtaaggagat
    57841 cggtcggcca gacgatgaca cccagtggct ggttactggc tttaaccgcc agccgaagca
    57901 acccgtaaag gaacccgacg cgccagttat cgtcgtggaa tctgctgacg agttgttggc
    57961 gctgttaaca tcagtacgtc agggaatgac ggcaggagaa gtggctgccc actttggctg
    58021 gccgctggaa aaagccagaa atgcgctcga acagctcttt tctgccggga cgttacgtaa
    58081 acgcagtagt cgttatcgtc tcaagcccca ttaacctgtc ggaggtgccg ggtgtcctac
    58141 aacacccggc attaacatca tgaaaaagac ccgtacagcc aatttgcacc atctttatca
    58201 tgaaccctta cccgaaaacc tgaagctcac gccgaaggtc gaagtggata atgttcatca
    58261 acgacagaca acggatgtct atgaacatgc tttgacgatt accgcctggc agcagattta
    58321 cgatcagctg catccgggca agtttcatgg tgaatttacg gaaattctac tcgatgatat
    58381 tcaggttttt cgtgaataca ccggtctggc gctgcgtcag tcgtgcctgg tctggccgaa
    58441 ctcgttctgg tttggcattc cggcgacgcg cggtgagcag ggatttatcg gttcgcaatg
    58501 tctgggaagc gcagaaatag cgacgcgccc tggtggaacc gagtttgaat taagtacgcc
    58561 ggacgattac acgatcctgg gcgtagtgct ttctgaagat gtcatcaccc gtcaggctaa
    58621 ctttttgcat aatccggatc gggtattaca tatgctgcgt agccagtcgg cgctggaagt
    58681 gaaagaacag cataaagccg cgctgtgggg ctttgtccaa caggcgctgg cgacgttttg
    58741 cgagaatccc gagaatttgc atcagcctgc ggtgcgaaaa gtgctggggg ataatttgct
    58801 catggcgatg ggggcgatgc tggaagaagc gcagccaatg gtgacggcgg aaagcatcag
    58861 tcatcagagt taccgtagat tactttcccg cgcccgtgaa tatgtgctgg aaaacatgtc
    58921 cgaaccggtg acggtgctgg acttgtgtaa tcaactgcat gtcagtcgcc gcacgctaca
    58981 aaacgcgttt cacgctattt taggcattgg cccgaacgcg tggctgaaac gcattcgcct
    59041 gaacgccgta cgccgcgaac tgataagccc gtggtcgcaa agcacaacgg taaaagacgc
    59101 cgccatgcag tggggattct ggcatctggg gcaatttgcc acggattacc agcagctgtt
    59161 tgccgagaag ccgtcgttga cgttgcatca gcggatgcgg gaatgggggt gagggagtta
    59221 cacccaatcc ctgaccttaa taaactcact caacgccgct tccgggctgc ctgcttctgg
    59281 ctgataatcg tactcccagc gtaccagcgg cggcattgac atcaggatag actcggtgcg
    59341 cccgccggtt tgcaggccaa acagtgtgcc acgatcccag accagattga actcgacata
    59401 acgaccacga cgatataact ggaaattgcg ctcgcgctcg ccgtaggcca tcgttttacg
    59461 tcgctctaca attggtaaat aagcgttggt gtagcctttg cctaccgcct gcataaaggc
    59521 aaaacagtgg tcaaagtctg gcgtgttcag gtcatcaaag aacaggccgc caatgccgcg
    59581 ttgttcgtta cgatgtttga ggtagaagta ttcgtcgcac cactttttgt aacgcggata
    59641 aacgtcttcg ccaaatggct ggcacaggtc acgggcggtg cggtgccagt gaatagcatc
    59701 gtcttcaaag ccatagaaag gggttaagtc gaagccgccg ccaaaccacc agacgggttc
    59761 ggcacccggt ttttcggcaa taaaaaagcg cacattcgcg tggctggtgg gaacatacgg
    59821 attatgcgga tgcactacca gtgaaacgcc catcgcctcg aaactgcgcc cggcaagttc
    59881 cgggcgatga gcggtggcgg aagcaggcat cgcctcaccg tggacatgcg aaaagttaac
    59941 gcccgcctgt tcgaaaacac caccattacg caacacccgg ctacgtccgc cgccgccagc
    60001 ttcgcgctgc cagctatctt cgacaaattc tgtgccatcg acggtgctca actgctgaca
    60061 aatcgtatcc tgaaggttga gcagaaactg tttaacctgg tgtgcgtcgg gtttcatcac
    60121 ttaccgcttt ctcgagtgtg ctttctggtt gtcaaaccag tggaaaaaac tgatcacgcc
    60181 ttcggcaatc gctgtggcga ttttctggcg aaatgccgcc gtgcctaaca gccgctcttc
    60241 ttccgggttg gtgataaacg aagtttccac cagcaccgaa ggaaccgacg gcgacttcaa
    60301 caccacaaac gcggcttgtt cggtgttgcg gctgtgcagt ttatgcaccg gcttaatctt
    60361 cttcagaata tgcgagccga gcgtcaggct gtttttaata gtatcggttt gcaccagatc
    60421 aaacagcact tgctgcaata ggtgatcctt gtcagtcgcc tttttaccag caacttcatc
    60481 ggcgcggttt tcgcgttcag acaggtattt cgccattgcg ctacttgccc cacggttaga
    60541 gagggcaaat accgaagcac cggcagcttt cgggttggta aagccgtcgg catgaattga
    60601 cataaacaga tctgcgccat gtttatgggc gatttcaacg cgatcgtata gtgggataaa
    60661 cgtatcgcca gaacgcgtta aacgcgcatc aatgccatga ttacgcaaaa tggaacggac
    60721 gtttttagca atcgccagca ccacatgttt ttctttcgaa ccgttgcgtc cgatcgctcc
    60781 ggtatcaatc cctccgtggc ctggatcgag aacaacgaca cgtttgccgc cagatttttt
    60841 ggctttcggc ttgctgtgtc cgttgctggt ttttaaaggt tcgtctttgg cgatggcttg
    60901 cgacattcct gacaacgtca gggcagccaa accggctttc agcacctggc ggcgcgaagt
    60961 gagtgttttt agtggtttaa aagtgctcat acggcctgag ttgtaataat aaagttccag
    61021 atgttatatc gtatcgcgta atccgttacg accgtttgct gatgagaatt gttttacttt
    61081 tcatttcaat agttgacaac tccccattat gccacttttt caaccgattt tcgtcagata
    61141 ttggctaatt cgaccgttca cgccataatc agccttttaa cccgcaaaaa ggtaacgaat
    61201 accatggaga tacgcgtatt tcgccaggaa gatttcgaag aggtcatcac cctttgggag
    61261 cgttgcgact tgctgcgtcc gtggaacgat ccggaaatgg acatcgagcg taagatgaac
    61321 catgacgtca gtttgtttct ggtcgctgag gtaaacggtg aagtggtcgg aacggtgatg
    61381 ggcggttatg acgggcatcg cgggtctgct tattatcttg gcgtgcatcc agagtttcgt
    61441 gggcgtggga ttgccaatgc gttgcttaat cggttggaga aaaagctgat tgctcgcggc
    61501 tgcccgaaaa ttcagatcaa cgtgccggaa gataacgaca tggtgctcgg aatgtatgaa
    61561 cgcctgggat atgaacacgc cgacgtgctg agtttgggta agcgtttgat tgaagatgaa
    61621 gagtactgag tttcatcctg tccattatga tgcgcacggt cgcctgcgtt tacccttgct
    61681 cttctggctt gtgctattgc ttcaggcgcg aacctgggtg ctgtttgtca ttgccggtgc
    61741 gtcgcgcgag cagggtactg cactgctaaa tctgttttat cccgatcacg ataatttctg
    61801 gctggggttg attcccggca ttccggcggt gctggcgttt ttgctgagtg gtcggcgggc
    61861 ttcatttccc cgtatctggc atgtgctcta ttttctgttg ctgttggcgc aggtggtttt
    61921 actttgctgg caaccgtggc tgtggctgaa cggtgaatcc gttagcggta tcgggctggc
    61981 gctggtagtg gcggatattg tggcgttaat ctggctgctg accaatcgac gtttacgcgc
    62041 ttgttttaat gaagaaaaag aataacggca ctttttggtg aatttgcact ccaagcaacg
    62101 ttattgaata accaaaggaa gtgacatgat atcgctgcgt ttaatgttat gcgctatgcc
    62161 gttgatgctg accggctgtt ccacgatgtc gtcagttaac tggtctgccg ctaacccgtg
    62221 gaactggttt gggtcgtcca ccaaagtgag cgagcagggc gttggtgaat taacggcgtc
    62281 cacaccactg caagaacaag ccattgctga tgcgcttgat ggcgattatc gcctgcgcag
    62341 cggaatgaaa accgcgaacg gcaacgtggt gcgctttttt gaagtgatga aaggcgacaa
    62401 cgtggcgatg gtgattaacg gcgatcaggg cacgatcagc cgcattgatg tgctggatag
    62461 cgatattcca gctgactcca gtgttaaaat cggtacaccg tttagcgacc tttacagcaa
    62521 agcatttggc aattgccaaa aagctgatgg tgatgataat cgtgccgtcg aatgtaaagc
    62581 cgaaggcagt caacatatta gctaccagtt cagcggggaa tggagtggtc ctgaagggtt
    62641 aatgccttcg gacgataccc tgaaaaactg gaaagtcagt aaaattatct ggcggcgtta
    62701 atttgcgtct gaacaaaccg ccgccgcgaa aaaacacgta aaatagcgcc caacaatgcc
    62761 acggattgcg tggcattctt attttcagga ggaataatgt ctcaggttca gagtggcatt
    62821 ttgccagaac attgccgcgc ggcgatttgg atcgaagcca acgtgaaagg ggaagttgac
    62881 gccctgcgtg cggccagtaa aacatttgcc gataaactgg caacttttga agcgaaattc
    62941 ccggacgcgc atcttggtgc ggtggttgcc tttggtaaca acacctggcg cgctctgagc
    63001 ggcggcgttg gggccgaaga gctgaaagat tttccgggct acggtaaagg ccttgcgccg
    63061 acgacccagt tcgatgtgtt gatccacatt ctttctctgc gtcacgacgt aaacttctct
    63121 gtcgctcagg cggcgatgga agcctttggt gactgcattg aagtgaaaga agagatccac
    63181 ggcttccgtt gggttgaaga gcgtgacctg agcggcttcg ttgacggtac tgaaaacccg
    63241 gcgggtgaag agacgcgccg tgaagtggcg gttatcaaag acggcgtgga tgcgggcggc
    63301 agctatgtgt ttgtgcagcg ttgggagcac aacctgaagc agctcaaccg gatgagcgtt
    63361 cacgatcagg agatgatgat cgggcgcacc aaagaggcca acgaagagat cgacggcgac
    63421 gagcgtccgg aaacctctca cctgacccgc gttgatctga aagaagatgg caaagggctg
    63481 aagattgttc gccagagcct gccgtacggc accgccagtg gcactcacgg tctgtacttc
    63541 tgcgcctact gcgcgcgtct gcataacatt gagcagcaac tgctgagcat gtttggcgat
    63601 accgatggca aacgtgatgc gatgttgcgt ttcaccaaac cggtcactgg cggctattac
    63661 ttcgcgccgt cgctggacaa gttgatggcg ttgtaatcgt ttctgattcg atgatttaag
    63721 gccagccagt atcacaactg ac
//
LOCUS       DAPOZO010000016        62944 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPOZO010000016 DAPOZO010000000
VERSION     DAPOZO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.016"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(23..931)
                     /gene="mak"
                     /locus_tag="ROI21_001074"
     CDS             complement(23..931)
                     /gene="mak"
                     /locus_tag="ROI21_001074"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDX5110027.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGLRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            1056..1967
                     /gene="rdgC"
                     /locus_tag="ROI21_001075"
     CDS             1056..1967
                     /gene="rdgC"
                     /locus_tag="ROI21_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDX5110028.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(2045..2128)
                     /locus_tag="ROI21_001076"
     CDS             complement(2045..2128)
                     /locus_tag="ROI21_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110029.1"
                     /translation="MTQRPWSKLQRKTHNIAALKIIARRSE"
     gene            complement(2125..2466)
                     /locus_tag="ROI21_001077"
                     /pseudo
     CDS             complement(2125..2466)
                     /locus_tag="ROI21_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(2614..2898)
                     /gene="ppnP"
                     /locus_tag="ROI21_001078"
     CDS             complement(2614..2898)
                     /gene="ppnP"
                     /locus_tag="ROI21_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDX5110030.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(2970..3647)
                     /locus_tag="ROI21_001079"
     CDS             complement(2970..3647)
                     /locus_tag="ROI21_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDX5110031.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(3905..4096)
                     /gene="yaiA"
                     /locus_tag="ROI21_001080"
     CDS             complement(3905..4096)
                     /gene="yaiA"
                     /locus_tag="ROI21_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDX5110032.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(4146..4670)
                     /gene="aroL"
                     /locus_tag="ROI21_001081"
     CDS             complement(4146..4670)
                     /gene="aroL"
                     /locus_tag="ROI21_001081"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDX5110033.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(4853..5311)
                     /locus_tag="ROI21_001082"
     CDS             complement(4853..5311)
                     /locus_tag="ROI21_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDX5110034.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5431..6240
                     /gene="proC"
                     /locus_tag="ROI21_001083"
     CDS             5431..6240
                     /gene="proC"
                     /locus_tag="ROI21_001083"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDX5110035.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(6257..7372)
                     /gene="adrA"
                     /locus_tag="ROI21_001084"
                     /gene_synonym="dgcC"
     CDS             complement(6257..7372)
                     /gene="adrA"
                     /locus_tag="ROI21_001084"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDX5110036.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNDHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(7474..7794)
                     /gene="psiF"
                     /locus_tag="ROI21_001085"
     CDS             complement(7474..7794)
                     /gene="psiF"
                     /locus_tag="ROI21_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDX5110037.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDAHKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(7913..9328)
                     /gene="phoA"
                     /locus_tag="ROI21_001086"
     CDS             complement(7913..9328)
                     /gene="phoA"
                     /locus_tag="ROI21_001086"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDX5110038.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVSHVTSRKCYGPSVTSEKCTGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAADTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(9429..9689)
                     /gene="iraP"
                     /locus_tag="ROI21_001087"
     CDS             complement(9429..9689)
                     /gene="iraP"
                     /locus_tag="ROI21_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDX5110039.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            complement(9871..10074)
                     /locus_tag="ROI21_001088"
     CDS             complement(9871..10074)
                     /locus_tag="ROI21_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110040.1"
                     /translation="MLQSSADKACNVLVKKARYLNDVRKITKMMNGKLIYSWLVWPCS
                     EFFSLNYKFTKRIYFITFIYNKG"
     gene            10152..11246
                     /gene="ddlA"
                     /locus_tag="ROI21_001089"
     CDS             10152..11246
                     /gene="ddlA"
                     /locus_tag="ROI21_001089"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDX5110041.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(11270..11482)
                     /locus_tag="ROI21_001090"
     CDS             complement(11270..11482)
                     /locus_tag="ROI21_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDX5110042.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            11742..12050
                     /locus_tag="ROI21_001091"
     CDS             11742..12050
                     /locus_tag="ROI21_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDX5110043.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(12108..13202)
                     /gene="yaiW"
                     /locus_tag="ROI21_001092"
     CDS             complement(12108..13202)
                     /gene="yaiW"
                     /locus_tag="ROI21_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDX5110044.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGKVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(13215..14435)
                     /gene="sbmA"
                     /locus_tag="ROI21_001093"
     CDS             complement(13215..14435)
                     /gene="sbmA"
                     /locus_tag="ROI21_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDX5110045.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            14787..15944
                     /gene="ampH"
                     /locus_tag="ROI21_001094"
     CDS             14787..15944
                     /gene="ampH"
                     /locus_tag="ROI21_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDX5110046.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(15945..16568)
                     /gene="iprA"
                     /locus_tag="ROI21_001095"
     CDS             complement(15945..16568)
                     /gene="iprA"
                     /locus_tag="ROI21_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDX5110047.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(16654..19602)
                     /gene="ehaB"
                     /locus_tag="ROI21_001096"
     CDS             complement(16654..19602)
                     /gene="ehaB"
                     /locus_tag="ROI21_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HDX5110048.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYNGKGWNADDIALAVIAHPAAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILNVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSISGLFQSGDDYQLSNNMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
     gene            20128..21102
                     /gene="hemB"
                     /locus_tag="ROI21_001097"
     CDS             20128..21102
                     /gene="hemB"
                     /locus_tag="ROI21_001097"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDX5110049.1"
                     /translation="MTDLIQRPRRLRKTPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRHALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(21142..21993)
                     /gene="tauD"
                     /locus_tag="ROI21_001098"
     CDS             complement(21142..21993)
                     /gene="tauD"
                     /locus_tag="ROI21_001098"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDX5110050.1"
                     /translation="MSERLSITSLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLREQAITPLQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(21990..22817)
                     /gene="tauC"
                     /locus_tag="ROI21_001099"
     CDS             complement(21990..22817)
                     /gene="tauC"
                     /locus_tag="ROI21_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDX5110051.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAILLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(22814..23581)
                     /gene="tauB"
                     /locus_tag="ROI21_001100"
     CDS             complement(22814..23581)
                     /gene="tauB"
                     /locus_tag="ROI21_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDX5110052.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESCELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVRDNVAFGLQLAGVEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGEPSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(23594..24556)
                     /gene="tauA"
                     /locus_tag="ROI21_001101"
     CDS             complement(23594..24556)
                     /gene="tauA"
                     /locus_tag="ROI21_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX5110053.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            25165..25836
                     /locus_tag="ROI21_001102"
     CDS             25165..25836
                     /locus_tag="ROI21_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDX5110054.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            25846..27042
                     /locus_tag="ROI21_001103"
     CDS             25846..27042
                     /locus_tag="ROI21_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDX5110055.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSPKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            27020..27601
                     /locus_tag="ROI21_001104"
     CDS             27020..27601
                     /locus_tag="ROI21_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDX5110056.1"
                     /translation="MKNFLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDQQPVSVRTPEGIIATECDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPTGTYSLRQELIRTGD"
     gene            27603..28376
                     /locus_tag="ROI21_001105"
     CDS             27603..28376
                     /locus_tag="ROI21_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDX5110057.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVISSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQFAAHLTWKF"
     gene            28561..28836
                     /gene="frmR"
                     /locus_tag="ROI21_001106"
     CDS             28561..28836
                     /gene="frmR"
                     /locus_tag="ROI21_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDX5110058.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            28871..29980
                     /gene="frmA"
                     /locus_tag="ROI21_001107"
     CDS             28871..29980
                     /gene="frmA"
                     /locus_tag="ROI21_001107"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDX5110059.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            30074..30907
                     /gene="fghA"
                     /locus_tag="ROI21_001108"
     CDS             30074..30907
                     /gene="fghA"
                     /locus_tag="ROI21_001108"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDX5110060.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAASEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATARKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFTAYLGENKEAWLDYDPVSLISQGQRIAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(31032..31571)
                     /locus_tag="ROI21_001109"
     CDS             complement(31032..31571)
                     /locus_tag="ROI21_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDX5110061.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLIDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(31673..32884)
                     /gene="mhpT"
                     /locus_tag="ROI21_001110"
     CDS             complement(31673..32884)
                     /gene="mhpT"
                     /locus_tag="ROI21_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDX5110062.1"
                     /translation="MSTRIPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILISSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     TGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(33064..34077)
                     /gene="dmpG"
                     /locus_tag="ROI21_001111"
     CDS             complement(33064..34077)
                     /gene="dmpG"
                     /locus_tag="ROI21_001111"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDX5110063.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(34074..35024)
                     /gene="mhpF"
                     /locus_tag="ROI21_001112"
     CDS             complement(34074..35024)
                     /gene="mhpF"
                     /locus_tag="ROI21_001112"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDX5110064.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLVRKAGEAA"
     gene            complement(35021..35830)
                     /gene="mhpD"
                     /locus_tag="ROI21_001113"
     CDS             complement(35021..35830)
                     /gene="mhpD"
                     /locus_tag="ROI21_001113"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDX5110065.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIENAEAAYAIQRI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(35840..36706)
                     /gene="mhpC"
                     /locus_tag="ROI21_001114"
     CDS             complement(35840..36706)
                     /gene="mhpC"
                     /locus_tag="ROI21_001114"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDX5110066.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(36724..37668)
                     /gene="mhpB"
                     /locus_tag="ROI21_001115"
     CDS             complement(36724..37668)
                     /gene="mhpB"
                     /locus_tag="ROI21_001115"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDX5110067.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(37670..39334)
                     /locus_tag="ROI21_001116"
     CDS             complement(37670..39334)
                     /locus_tag="ROI21_001116"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDX5110068.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            39411..40358
                     /gene="mhpR"
                     /locus_tag="ROI21_001117"
     CDS             39411..40358
                     /gene="mhpR"
                     /locus_tag="ROI21_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDX5110069.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEILATRPGDDYQLAR
                     EPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGMHLR"
     gene            40435..41517
                     /gene="lacI"
                     /locus_tag="ROI21_001118"
     CDS             40435..41517
                     /gene="lacI"
                     /locus_tag="ROI21_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDX5110070.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPQALADSLMQLARQISRLESGQ"
     gene            41640..44714
                     /gene="lacZ"
                     /locus_tag="ROI21_001119"
     CDS             41640..44714
                     /gene="lacZ"
                     /locus_tag="ROI21_001119"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDX5110071.1"
                     /translation="MTIITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDECWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFV
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTTDGTLIEAEACDVGFR
                     EVRVENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEVALLQCTADILADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVTSDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAV
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            44766..46019
                     /gene="lacY"
                     /locus_tag="ROI21_001120"
     CDS             44766..46019
                     /gene="lacY"
                     /locus_tag="ROI21_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDX5110072.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVPAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            46084..46695
                     /gene="lacA"
                     /locus_tag="ROI21_001121"
     CDS             46084..46695
                     /gene="lacA"
                     /locus_tag="ROI21_001121"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDX5110073.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRVIREINDRDKHYYYKDYKVEPSV"
     gene            complement(46783..47964)
                     /gene="cynX"
                     /locus_tag="ROI21_001122"
     CDS             complement(46783..47964)
                     /gene="cynX"
                     /locus_tag="ROI21_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDX5110074.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHHDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPFCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(47970..48440)
                     /gene="cynS"
                     /locus_tag="ROI21_001123"
     CDS             complement(47970..48440)
                     /gene="cynS"
                     /locus_tag="ROI21_001123"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDX5110075.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(48471..49130)
                     /gene="cynT"
                     /locus_tag="ROI21_001124"
     CDS             complement(48471..49130)
                     /gene="cynT"
                     /locus_tag="ROI21_001124"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDX5110076.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTSIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            49240..50139
                     /gene="cynR"
                     /locus_tag="ROI21_001125"
     CDS             49240..50139
                     /gene="cynR"
                     /locus_tag="ROI21_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDX5110077.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFTPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGGHESR"
     gene            complement(50179..51462)
                     /gene="codA"
                     /locus_tag="ROI21_001126"
                     /pseudo
     CDS             complement(50179..51462)
                     /gene="codA"
                     /locus_tag="ROI21_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
     gene            complement(51452..52711)
                     /gene="codB"
                     /locus_tag="ROI21_001127"
     CDS             complement(51452..52711)
                     /gene="codB"
                     /locus_tag="ROI21_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDX5110078.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            complement(52948..54834)
                     /gene="prpE"
                     /locus_tag="ROI21_001128"
     CDS             complement(52948..54834)
                     /gene="prpE"
                     /locus_tag="ROI21_001128"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDX5110079.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMETIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(54874..56325)
                     /gene="prpD"
                     /locus_tag="ROI21_001129"
     CDS             complement(54874..56325)
                     /gene="prpD"
                     /locus_tag="ROI21_001129"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDX5110080.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILDVSLDRARLEQMPVNEYLDLYVI"
     gene            complement(56359..57528)
                     /gene="prpC"
                     /locus_tag="ROI21_001130"
     CDS             complement(56359..57528)
                     /gene="prpC"
                     /locus_tag="ROI21_001130"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDX5110081.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(57573..58463)
                     /gene="prpB"
                     /locus_tag="ROI21_001131"
     CDS             complement(57573..58463)
                     /gene="prpB"
                     /locus_tag="ROI21_001131"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDX5110082.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDELFARSQVK"
     gene            58702..60288
                     /gene="prpR"
                     /locus_tag="ROI21_001132"
     CDS             58702..60288
                     /gene="prpR"
                     /locus_tag="ROI21_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDX5110083.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALAAPFSSALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKIPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            complement(60389..60664)
                     /locus_tag="ROI21_001133"
     CDS             complement(60389..60664)
                     /locus_tag="ROI21_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX5110084.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            60811..61482
                     /locus_tag="ROI21_001134"
     CDS             60811..61482
                     /locus_tag="ROI21_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDX5110085.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(61499..61744)
                     /locus_tag="ROI21_001135"
     CDS             complement(61499..61744)
                     /locus_tag="ROI21_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110086.1"
                     /translation="MAVQLFITLLNQLPLLSSLQSGTLPLFGYSGRVRAMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(62157..>62944)
                     /locus_tag="ROI21_001136"
     CDS             complement(62157..>62944)
                     /locus_tag="ROI21_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110087.1"
                     /translation="LMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFKVLIKQYYDYP
                     YDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVF
                     RILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDCKCILLEHHMP
                     LLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLL
                     LSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT        15230 a        16766 c        15806 g        15142 t
ORIGIN      
        1 atgcgccaat tgcctacgtt ttttactctt gtggccataa ccacgcagcg ccgcgtacgc
       61 cgctggaatc accgtgcttc gccttacgca ccggtgtttc acattcgccg ccgaagacaa
      121 attgtttaat caactgcgga accgtttgat ataaacggtc tacattgctc atcccgcccc
      181 ccaggacaat cacatccgga tcgagaatat tcacgacatg tgccagcgat tttgccagcc
      241 gcagctcgta gcgacgcaat gccagttccg ctaccggatc gctttcttca accaggcgga
      301 taatttcact gcctttcagc gcatgtccgc tcaaacgacg ataatccgtc gcgaatcccg
      361 tgcccgaaat aaaggtttca atacaacctt gtttaccgca ataacaaggg acttcctcgc
      421 gataacgcag ttcgtcttcg tccatccacg gtagcggatt gtgtccccac tcacctgccg
      481 tgccattgcc gccgatatgc gcccgcccat tgaatgccac gcccgcgccg catcccgtgc
      541 cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc gccatctact gcttctgaaa
      601 ccgccagaca gttggcgtca tttgccagcc gcacttcccg ctgcaaccgc gcgcttaagt
      661 ctttatcgaa tggctgaccg ttgagccagg ttgaattggc attcttcacc acgccggtgt
      721 aaggcgaaat tgagccagga atgcccatac ctaccgttcc gcgcagccct gtcgcctgct
      781 ccgccatatc aaccaacgtg gcgatcgttt caatagtctg ccggtaatca tcacgcggcg
      841 tgggcagacg atggcggtac aactgctccc ctgcatcgcc cagtgcaatc acttcagttt
      901 tggtgccgcc taaatcgata cctatacgca cggtactctc cttatttttt tcaatatcaa
      961 tagcgtagag acggacaact ggattggcaa tgcaaggccg ccgacaattc gttatcatgc
     1021 ccgctaaatt taacgacaag gccgtggaaa ttatcatgct gtggttcaaa aatttaatgg
     1081 tttaccgtct tagccgcgag atttcgttgc gtgcagaaga gatggaaaaa cagctagcct
     1141 cgatggcatt taccccatgc ggcagccagg acatggcgaa gatgggctgg gttcctccga
     1201 tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg tcaaattgtt atctgcgcgc
     1261 gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca ggcgctggaa gcgaaaatcg
     1321 ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac cgaaaaagat tcgctgaaag
     1381 acgaagtgct gcactctctg ctgccacgtg ctttcagccg ttttagccag acaatgatgt
     1441 ggatcgacac ggttaacggt ttgattatgg tggactgcgc cagtgccaaa aaagcggaag
     1501 atacgctggc attactgcgt aaaagcctgg ggtcgttacc ggttgtacca ttgagcatgg
     1561 aaaacccgat tgaactgacg ctgaccgaat gggtacgctc cggcagcgcg gcacagggct
     1621 tccagctgct tgatgaagcc gagctgaaat cgctgctgga agatggtgga gtgatccgcg
     1681 cgaagaaaca agatctgacc agcgaagaga tcaccaatca cattgaagcc ggaaaagtgg
     1741 tgactaaact ggcgctcgac tggcagcagc gcattcagtt tgtgatgtgc gacgatggtt
     1801 cgctcaagcg tctgaagttc tgcgacgagc tgcgcgatca aaacgaagat atcgaccgtg
     1861 aagatttcgc ccagcgtttt gatgccgatt tcatcctgat gactggtgaa ctggcagcgt
     1921 taattcaaaa cctgattgaa ggattaggtg gcgaagcaca acgttaattg ctgatttacc
     1981 tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg tacgaagcca caccaggcat
     2041 ataattattc gctacggcgg gcaataattt ttagcgcagc aatattatgc gttttacgct
     2101 gtaacttgct ccatgggcgt tgtgtcattg tttttcctca agccggtgac gcgcttcact
     2161 gcgaattttc cgcgttgccc cggttttcac taaatcacgt aacagcgaaa gatcgggttg
     2221 atcgacaaat aagagtaatg atggatcatc ttttaaccac atcgttttca catcggcagc
     2281 caactgtgga ttattaataa ccaacgcccg atcctgaggc tcagtcaatg ccgttaacaa
     2341 cgacgccggg gtatgtgcgt tgcgcaatgc cgtttggcgc aaatgatcat tgcgactttg
     2401 cagcaatgca cgccaccatt catcatctga tttatctttt atcgtcgcca gttccgcaac
     2461 aatatctgga gatcttcgct gatatttata tcaaccttga tgaattatga atatatcgga
     2521 aatcgaaaaa atcgcagcaa aggaaatatt attatgggtt tatgagagaa catttcagaa
     2581 aaatgccctc ccgttcaagg ggaaggcgag gaattacaga tagcggcaca gataagaggt
     2641 gggttcggca acttgcagat gaaactcact gtgaccggga acattaaaca ccgaaccggc
     2701 ttcatacact tgccagtcgg tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac
     2761 cgtcatctct tccggctcag cggtgctgaa ggtgtattcg ccttcaacca taacacccac
     2821 gctggcgcga ccagtgctgc tgctggaaaa gccgatcgat ttcactttgc cggaaaagta
     2881 ctcattactt tgaagcataa actggccctg tctggtagtt gaacatctac ttcactatag
     2941 gggccagagg cgctggctgt cacgcaaaat tacaccagta attccgcagc cagccgtgca
     3001 atcaatacgt tagagagcaa gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga
     3061 cgctggttaa atcccaaaca atccagcatg atgacatcag ccccttttgc cagtaattct
     3121 ttcccggcat caatgatttt ttgttctgaa tcatgaatgg ggttacccaa tgaaaatact
     3181 ggcggtttct gcaaaatttg ccatttttgc gcctgaacgg tcagcaactc ctcaaccggt
     3241 acgataaccc ccacctgatg atcttcaaca atagaggaaa ccagcggtgg caatatccgc
     3301 gacggctcaa gaaagatcgt attacgcgcg gtcatactac taatgtttgc tgtactcatt
     3361 aatataatga cgtcgtaacc ctgattatcg agcacttcaa ccacaccttg caggtctcgt
     3421 tccactttac gacgcgaaac atgggccagc tggttgtcat ttaataatgt gagaatggtg
     3481 tcttcgcctg cttctggcgc gtactccgcc atcacttctt cgcggcttaa cttccccagc
     3541 aggctatgat gggaaatatt atcttcgtca atgtattctg tcaggagcgg caaaacttcc
     3601 tgcataggta caatgccgat ggttagaatc gccaacgacg cactcattgt tcttccacct
     3661 tacttttatt tcgtcgttaa ttaccgggca agcgctaagc accactgctg acacttacct
     3721 gtgctacaaa tgacaaaaag cgtagcagca gacctgcacg gcataatgta aagattccaa
     3781 atgattccag taatggattg ttattgttta atattctaat tatttgaaaa acatgaattg
     3841 tgaaaaatgt gacgcagatc ccttgtggtg gcactcagaa tcccttccga gcagtctgat
     3901 gcggtcactc tttgtcaggg tcctcatagc gttttttcgc atccatcgct tcctgagcgg
     3961 tcggatgttc actgatcaac gagtctggtt taggatgatc ggctctgagt tggtaccagg
     4021 ttaccgtctg tcctggcttt cctttttcaa tcgtcactat atatgcttca cgaggataag
     4081 gcggtttcgt tggcatcgtt cttccttatt tcacgggatg aacgttaagt ataggcgctc
     4141 gaaaatcaac aattgatcgt ctgtgccagg gcactgcgaa tttcagaaat cacctggctt
     4201 ggttcgtttg ttgcgtcgat gataatatgc gcaacttcgc gatatagcgc atcgcgttct
     4261 tccagcactt cctgaacttc ttcgctcagc ggtttccccg ttaaggttgg ccgtaaatct
     4321 tcttccggtg cagcttgcag tcggttaacc aggactgata ctggcgcaca caaataaacc
     4381 acgatcccgt tattttgcat aaagtgacga ttaaattccg tcagaataat gccgccgccc
     4441 gtggcgataa cggtggatgg cgcagttacc gcttccagcg ccgccgtttc tctggcgcgg
     4501 aatcccgccc actcttccct ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc
     4561 aaccactggt cggtatcgac aaaccgacgg ttaagcgaat cggcaagagc cattccgacc
     4621 gttgttttac cacagccccg aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt
     4681 ccccaatagg tcgcaatgcg gcgaaagccg gtgtcatgag agtagcgatc ataccatcaa
     4741 actagtacaa tttcgattgt aaagaaaaaa ttccacttaa agtgaaaatt ttaatacatc
     4801 ccttactata ccaataaatt ttcaagaatg aagtgtaaat aataaattac attcagccac
     4861 gactacgttg cacttccagc caccacttct ccagctccgc ggcaaaggcc tggcggtcac
     4921 gttgtgaaag gctatctggt cccccggtct ggatcccact ggcgcgtaag gtatccataa
     4981 aatcgcgcat cgtcaggcgc tcacgaatgg tcgctggcgt gtaacgttcg ccgcgtggat
     5041 taagcgccgc cgcgcctttc tcgatggctt cagcagccaa aggaatatct gcggtgatca
     5101 ccaaatcgcc agcttcacac tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg
     5161 cgacgcgcag cgttcgaata aatcgcgaag gcggtacgcg taaactctgg tttgctacca
     5221 gtaccagcgg catctgcata cgttcagccg cgcgatacaa aatctcttta attacattgg
     5281 gacacgcgtc ggcatccacc caaattgtca taaagtcatc ctttgttggg taattctcta
     5341 ttgtgtcgcg cttttgcctt ccggcatagt tctgtttatg cttctgccag cgattatcaa
     5401 aacaatgaat ttcacggcag gagtgaggca atggaaaaga aaatcggttt tattggctgc
     5461 ggcaatatgg gaaaagccat tctcggcggt ctgattgcca gcggtcaggt gcttccaggg
     5521 caaatctggg tatatacccc ctccccggat aaagtcgccg ccctgcatga ccagttcggc
     5581 atcaacgccg cagaatcggc gcaagaagtg gcgcaaatcg ctgacatcat ttttgccgcc
     5641 gttaaaccag gcatcatgat taaagtgctt agcgaaatca cctccagcct gaataaagac
     5701 tctctggtcg tttctattgc agcaggtgtc acgctcgacc agcttgcccg tgcgctgggc
     5761 catgaccgga aaattatccg cgccatgccg aacactcccg cgctggttaa tgcagggatg
     5821 acctccgtaa cgccaaacgc gctggtaacc ccagaagata ccgctgatgt gctgaatatt
     5881 ttccgctgct ttggcgaagc ggaagtaatt gccgagccga tgatccaccc ggtggtcggt
     5941 gtgagcggtt cttccccagc ctacgtcttt atgtttatcg aagcgatggc cgacgccgcc
     6001 gtgctgggcg ggatgcctcg cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt
     6061 tccgcaaaaa tggtgctgga aacgggagaa catccggggg cactgaaaga tatggtctgc
     6121 tcaccgggag gcaccaccat tgaggcggta cgcgtactgg aagagaaagg cttccgtgcc
     6181 gcagtgatcg aagcgatgac gaagtgtatg gaaaaatcag aaaaactcag taaatcctga
     6241 tgacttttgc cggacgtcag gccgccactt cggtgcggtt acgtccggct ttctttgctt
     6301 tgtaaagcgc caaatctgcc gatttcaacc actcacgata gtgactcatt tgtgggttca
     6361 gcggcgcaac ccccacacta atccgtaaag ttacctgtgg cgtattcggc agacgtaatg
     6421 tatttagccc ttcatgcact cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg
     6481 acatgattac cgcaaactca tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg
     6541 ttatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca tggccccagg
     6601 tatcgttgat gctcttgaaa tggtcgatat cgataatcag taatgtcgca tcgcggttat
     6661 gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt cggttataca
     6721 cgcccgtcat accgtcgcgg gtactcatga cctgcaacct gcgtttatgt tccgccagtt
     6781 tagtcgccgt ctggtagctg acccagccaa acagcagagg ataaatgacg ataatgggaa
     6841 gggagagcca ccattccagc ggcgcactat tgaacgacac ggtgatgccc gtcagttcga
     6901 gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga cgggggccgc
     6961 ctgcccccat caaattcaga cacataatca tcaacatcgc ggtggaaggc agcacgttta
     7021 cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag ttgtaaattt
     7081 cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc ggccagacga
     7141 acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa accagcgttg
     7201 aagcaatcgg taagaacatg ccagccaggc caaccgcgcg gggtagtctg acgcgacggg
     7261 cgaagcgcag cccggatcgc tgatgatcgt tttgaggagt taaaggaggt tcctccccat
     7321 gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac attcagaaac
     7381 aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc gggtgagcca
     7441 ttttcgtagc tcacccgtta gcactatcag atatcaggcg gctttcttga ggcaggcgct
     7501 cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat tattacattc
     7561 gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact tgctgttctt
     7621 caggcaatca ctcatgtagg tcttatgagc atcccctttc aacgcctgcg ccgtcgcctg
     7681 ctgattacag gaggtcatac gctgttgttg cggggttaag gttctctcgg cagcgccgac
     7741 ggtggttaaa aaaaccagtc cgaaaagcaa ggtaaccagt aatgttattt tcatagcacc
     7801 atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt aagtctggtt
     7861 gctaacagca aaaaaaccac ccgacagcga aaattcaccg ccgggcgcgg ttttatttca
     7921 gccccagggc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt ccaacgacat
     7981 tggcagcatg tgggccatac gccgcaatac gcaactgact gccggtatgt tcttgtgaat
     8041 cctcttcgga gttcccgtaa ctcatcacca tcactgcgcc atctttggta tttagcgcct
     8101 gggtgaggcc cggcgctttg gtgtccgccg cgacaatctg gctggcgtgg gcgtgatcag
     8161 cggtgactat gaccagcgtg ttgccatcct ttttagcgaa ttccagcgca cgttgtacgg
     8221 cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg tgatcctgtt
     8281 tatcgatgga tgcaccttca acttgcagga aaaagccttt ctcattttta ctcaacaatt
     8341 caatggcttt gtcggtcatc tgcgccaggg tcggtacact gtcattacgt tgcggattag
     8401 gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc ggtcctagcc
     8461 agcgcactgg catattgccg tcagcaaaca gtcctagcag gggtttttgc tggttcgctt
     8521 ccgtcaccgc atttagcgag gcagcatcac tcaccaactg ataaccacgc gcctgtgcct
     8581 gttcacgcag cgtttttccc tgccattcac cggcggttgc cgtttcagca aaggtttttg
     8641 cgccgccgcc aagcgtaaca tcggcacgag cgttaagcag ctgttcggta atcgatcctt
     8701 tcccgccttt ttccagagcg ttacccgtac atttttcact ggtcacgctc ggaccgtagc
     8761 atttgcgcga ggtcacatgc gacaccagcg cagcgggcgt agcatcctgc aactctgcgg
     8821 tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc gttgggtgat
     8881 ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacacca gttgaccagg
     8941 cggttgctga tgcagccgag tcggtgacgt agtccggttt accggttttt ttattcagcg
     9001 catagtgagt gtattgcccg gtaagcggta atgcatcgat acctttaaaa aagccgcccg
     9061 caccttcggc ataattgcgt gcagcagtaa tttccgagtc ccccatccca tcgccaatca
     9121 gcaaaataat atttttggcg ggtttatcgc taagagaatc acgcagagcg gcggtctgat
     9181 cacccgttaa gcggcgagca ccgccgggtg cagtaatatc tccctgagca gcccggtttt
     9241 ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt aacggtaaga
     9301 gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat acattaaaaa
     9361 ataaaaacaa agcgactata agtctcggcc gtgacagctt tatgacagct gttgaaaaga
     9421 ttaacttttt actgacgagg atgcttcaat aacttcttta cataattgcg cagcagctcc
     9481 gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc ctctacctga
     9541 tcaatcaacc tttgttggtc attttgcgcc atattgcgaa gcattgcagt gacgataatc
     9601 tccaaagctt ctacctgcgc gcacagttct ttcgactctt cttctttttg ggcaagctta
     9661 aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa gtatggagaa
     9721 gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat gacaaaggaa
     9781 atactttgcg caaatatttt ttgtttgatt accagcgaaa cgtttctctt attttaataa
     9841 ttacccacac aaaatatagg cttttaatta tcaaccttta ttataaataa aagttatgaa
     9901 atatattctc ttcgtaaact tataattaag agaaaaaaac tcactgcatg gccatacaag
     9961 ccaggaataa attagcttcc cgttcatcat ttttgtgatc ttgcgcacat cattgagata
    10021 acgcgctttc ttcaccagta cgttgcacgc tttgtcagca gaagattgca acattctgtc
    10081 agaaggccgc gaatcccccg ttttgtcgtg gtaatgtatg cactttgtgt tagacgggat
    10141 aggtttttaa gatggaaaaa ctgcgggtag gaatcgtttt tggtggtaaa tcagcggaac
    10201 atgaagtgtc tctgcaatcg gcaaaaaaca ttgtcgatgc cattgataaa agtcgcttcg
    10261 acgttgtgct gctgggcatt gataaacaag ggcaatggca cgtcagcgat gccagcaatt
    10321 atctgctaaa tgcagacgat cctgcccata ttgcgttgcg cccttcggcg accagccttg
    10381 cgcaggtgcc tggtaaacat gaacatcagc ttatcgacgc gcaaaacggt cagccgttgc
    10441 cgacggtgga tgtcattttc ccgattgtcc acggtacgct gggcgaagat ggttccttgc
    10501 agggaatgct gcgggtcgcc aatttaccgt ttgtaggttc tgatgttctg gcttcagcgg
    10561 cctgtatgga taaagatgtc accaaacgtc tgctgcgcga tgccgggctg aacattgcgc
    10621 catttattac cctgacgcgc gctaatcgtc acaacatcag ttttgccgaa gtggagtcta
    10681 aactggggtt accgctgttt gtaaaaccgg ctaatcaggg ctcttctgtt ggtgtcagca
    10741 aagtaaccag tgaagaacag tacgcaattg ccgtcgatct ggcgttcgag ttcgaccata
    10801 aagtgatcgt tgagcaaggg atcaaaggtc gtgagatcga atgcgcagtt ctgggcaacg
    10861 acaatccgca agccagcacc tgtggcgaga tcgtactcac cagcgatttt tatgcctacg
    10921 acaccaagta cattgacgaa gatggcgcga aagtggtagt tccggcagcc attgcgccag
    10981 aaatcaacga taagatccgg gcgattgccg ttcaggctta tcagacgttg ggatgcgcag
    11041 gcatggcgcg tgtagacgtg tttttaaccc cagagaacga agtggtgatc aacgagatca
    11101 acacactgcc tggcttcacc aacatcagta tgtatccgaa gctgtggcaa gccagcggtc
    11161 tgggttacac cgacctgatc acccgtttga ttgagctggc gctggagcgt cacgctgcgg
    11221 ataacgcatt gaaaaccaca atgtaatatt taaacgcccg ataatattct tattgggcgt
    11281 tttcagtttc ttcatcgcga cgacgaataa tcagccccgc cagccagaaa ctaatcaccc
    11341 acgtcaccag accgacggca taggtctgcc aaccttttgc ttcaaatccc agtaacccca
    11401 ccacgccatt aagaatgaat ataaggccaa tggcgaacgc atagtagtgc cagtcacggc
    11461 ggatttttac aggcaagttc atggcagctc cgttaacgaa aaacaacatc cttgcatatt
    11521 ctgcaacatc cgtctgtggc gtagacaaca tttccgtaat tgttaataaa tttaacttaa
    11581 ggttgtcatt ttgtgactta cgacgaagat tcacgcttcg ggaatattcc caacatcaat
    11641 ttaccatgat tctgatattg acaatcttaa tgacctgtca gacttgccat aagccgctgg
    11701 tatcacacca gaagagaagc agatgacttc cggaggtttt tatggctgat ttcaccctgt
    11761 caaaatcgct gtttagcgga aaatatcgca atgcctcttc aacgcctggc aatattgcct
    11821 atgcgttgtt tgtgctgttt tgcttttggg ctggggcgca attgctgaac ctgctagtgc
    11881 atgcgcccgg cgtctatgag cgtttaatgc aggtccagga aacaggtcgc ccacgggtgg
    11941 aaattggttt aggtgtcggc accattttcg ggctgatccc gtttttagtt ggctgcctca
    12001 tttttgcagt agtggcgcta tggctgcact ggcgacatcg ccgccagtaa gatttacttg
    12061 ccagatagga cctaaaacgc tttatctggc atgacattcc tgtttgatca ctgtttcata
    12121 caacgcgccc gccgttcgtc tacgcgcttc gcaaaccagg ccgtagtcag gttgcgggtg
    12181 attttcgggc tttccagttg aatgccaggc aacatttcgc ggggtagtga tttacccgtt
    12241 ttcgcttcgg caagttgata aactttcttg tacagcgccg tctcctcaaa agagaagctg
    12301 tcccctttct ctaactggcg gcgaatttca ctgtcactca tccccagttt tcccgccagt
    12361 ttgcgcgttg ccagttcggt tttcccgggc tctttactgt cgtaacgaat taaatcaccg
    12421 tccagtgcca gcttcacgcc gctggcttta ctgaccgcgt tctgaaacgc ggcattacgg
    12481 ctggcgtacc agccagcgtt aaaatcagca aaacggtata tcggcgcgct atagctggcg
    12541 ggataattca gtaaatggta agtaccaaac cacaacccgc cgcgacgact gaagacttcc
    12601 tgacggactt taccgtccat tttccacgga taccctttgg tatgctgttc ggcaaaggca
    12661 atgctgacct gcatcggccc accggtgcgc accggattaa gcgaaccaaa caacgtctgc
    12721 cccattggca ccatgctgat cagatcgtca aaaatcgcgc tcaattgctt ttctgtacgc
    12781 accgaatcta accgttcgct atagcttttg ccgtttggcg atttgatttt tagcgcggta
    12841 tgcaccagaa aagcaggaat gtgcatccgt tcagcacgac ggtcaatctc ttgccaggcg
    12901 atcttgctta agcccggaac ggccggatct gcctgataat ttgactcctg ctgtgccacc
    12961 gccagcaccg agcagacgtt ttccagcgtc ggtgccagcc cctgactttc aaacgtggtg
    13021 gcgagatctt tcgcccaggc atcgcggtct ttcacgctcg caggcatctt ctgccgcacg
    13081 acgctcgcca catctatcgc tttttcgccc tttttcaacg gttgcggagc ctgactacta
    13141 caccccgcta aaaccagaac cgccaacaat gacagcgaag atagatgatt gacgcgcgac
    13201 attacttctc ctttttagct caaggtatgg gttacttcct gaattttgtc accatccagc
    13261 tcatgttcaa agctgcgcag acgtttgtag atagacatca actcaaccag tgtggtccat
    13321 gagttaatca ggtactggaa agctccgcga acctgaccaa aaacgttggt aatctgcgtc
    13381 atcaggccga gcgtaatcgt accggcaaca attgacggaa acagcaagaa caaaccgaaa
    13441 acgttatcga cctgcaaata gagaatgcgg gcgatgttga aatacatata gtgaaaatag
    13501 aggcggaaat agtttttccg tacggcgcta aacagctcgc gtaccgtagg cggcgtcgcg
    13561 cgcgtggcat cgtcttcacc ataaaccagc tctttacggt aggcagcctc tacacgctgg
    13621 tttttaaact ccagccccgg cagtttgatc cctaccactg ccagcaatcc ggtccccatt
    13681 agcgaccaga cgatagcggc aatcaccagg ccatacggaa tgtgcccgac aatcggcagc
    13741 tccggcacat gcgcggagag cgttaccagc accggcagga aggcgatcaa cgtcatgata
    13801 gcgttgataa agctgacgcc catattctcc agcgttgaag caaaacgcat ggtatcttcc
    13861 tgcacacgtt gggcggcacc ttcgatatga cgcagttgtt gccagttcgc catgtaatat
    13921 tcgttcatcg ccgtacgcca gcggaacacg tagtgactga caaagaagtt gttcagcaca
    13981 ctgatcacta ccgcgatcag cgcaatcccc agaaagacgc ccacttcgcg gtaaaattgt
    14041 tcgatggtga ctttatgcgg cgaacttagc gcggtttgaa tcagatcata gaacggcgca
    14101 taccaggcgt tgacggcgac cccgacttcc accaaaaacc aggtgacgaa gatgatcagt
    14161 gcagtaccga gtattgacca gtattgccaa cgatgcgggc tgtagataaa ccagaacaat
    14221 gcaaaaagtc ctacacaaac aatgtagtaa gcgtaaaaaa tcagaaaatc caacgaccag
    14281 aaacgcgcgg cgctaatcgg gatctgcccg gaagctccgg tgatacgcgc cacccagtca
    14341 cccccacctg cttgccagaa gataacggca atcaatgccc aaacaaaggc cgagagaaaa
    14401 aacgttcccg gctttgggaa aaaagactta aacatatgca ctcctgctaa cttcttatcg
    14461 ttttaacaac tgctgtgtat taaccgcatg accgctacaa agagtcagcc aaatgaaacc
    14521 gcccattgtg acccatagca aggcgcttag ttcgccagcc tgagcataag aattgtttcg
    14581 acggatttca caaaccaaag attgtttaca taagcggatt aacacgcgca ggatcacaaa
    14641 tgcaggcatt ggtgagcagg aaaaagatcg tgtttacgcg ccgttacgtt tttaacacta
    14701 ttattgctct aattcgccct ttgcaatcca tcaatgagta gtataaatac gctcagttac
    14761 cttcattcaa tctatggaca ccaccgttga aacgtagtct gcttttttct gccgtgctgt
    14821 gtgcggcgtc attgacctcc gtccatgcgg cgcagccgat cactgaaccg gagttcgcct
    14881 ctgatattgt cgatcgttat gccgatcata ttttttacgg cagcggtgcc acggggatgg
    14941 cgctggttgt tatcgacggt aaccagcgcg tctttcgcag ttatggcgaa acgcgacctg
    15001 gtaataacgt tcgcccgcag ctggattcgg tcatacgtat tgcttccctc accaagctaa
    15061 tgaccagtga gatgctggtg aaattgctcg accaggggac cgtgaagtta aacgatccgc
    15121 taagtaaata tgctccgcca ggcgcacgcg tgccaaccta caatgggacg cccattacgc
    15181 tggtcaatct ggcaacccat accagcgccc tgccccgtga acagcccggt ggcgcggcac
    15241 atcgtccggt gtttgtctgg ccaacgcgcg agcaacgctg gaaatacctt tccactgcca
    15301 aactaaaagc cgcgccgggc agccaggccg cgtactccaa cctcgcattc gatctgctgg
    15361 cagatgcact ggcgaatgcg tccggcaagc cttataccca gttgtttgaa gagcagatta
    15421 cccgtccatt ggggatgaaa gacaccacct acaccccctc gccggatcag tgccgccgtt
    15481 taatggttgc tgagcgtggt gccagtccgt gcaataacac gctggcggca attggcagcg
    15541 gcggcgtcta ttccacgcct ggcgatatga tgcgctggat gcagcagtat ttgtcatccg
    15601 atttttatca gcgcagtaac caggctgatc gcatgcagac gttgatttat cagcgcgcgc
    15661 agtttacgaa agtgattggc atggatgtcc ccggcaaagc ggatgcgctt ggtttaggct
    15721 gggtatatat ggccccgaaa gagggtcgtc cggggattat tcagaagaca ggtggtggtg
    15781 gcggtttcat tacctatatg gcgatgatcc cgcagaagaa tattggcgcg tttgtggtgg
    15841 tcactcgttc gccgttgacg cgctttaaaa atatgagcga tggcatcaat gacctggtga
    15901 ccgagctaag cgggaataaa ccgttggtta tccccgcatc ctgattaata ctctgaaggc
    15961 aaacggttga tagcgaccag tttgccttta ttcatttcga tatagccgcc tttacgcaaa
    16021 gcagcgagaa cttctgcgac gacagaacgc gatatgcgtg tacgttgatg gatataattc
    16081 atcaccccaa tacgtgatcg caattcttca ttccagtcaa tcattgataa taacgtcgcg
    16141 cggatttgtt cgtaggaatt atgcccaatg agctgcacgt cgcgtaattc cagaattcta
    16201 ttttgccagg ctaaccagta aaaagcatct cgccagagtt gattttgttc aataagggta
    16261 atggtttgtt tggctggtag atgatatccc gtacaatttc cttctgatat taatttgtat
    16321 ggtatatcgt ttttcattaa accatcagcc agtcccataa tataaggagc ctgggtaata
    16381 ccgataagta cgttttcttc tctacgcaga gagataacgc cctctaaaat aacgaaagta
    16441 tcttcgctat tgacatcact ggaaaatata acttgctttt cattattaaa ctcgaagcgc
    16501 gtgccatatc tggacaaaca tttatcgagc ttaccaaatt cctgaagagg tttaactaca
    16561 gataacattt gcgcgtcctt tgcagtaatg cccgtcaaat ccttgacggg cattatttag
    16621 attaaataac ctatttcttc agagtgaaga atattaccag gtatatttaa cacccacgtt
    16681 cgcggaccag tcttgatcta cgtcaccacc accgaggtag ttagcatcgg tataggcgct
    16741 gaagttcttg gtgaagctga actgagtacc cagaccaaca cgtaccgcag acccttcagt
    16801 accgttatcg atggaatcac cgttcacatc gttatcgttg ttagagtcgt cgtagacgta
    16861 agccagtttg aagtacggag tcagagcctg gtcttcgctg taggtgaagg tataacctgc
    16921 atctacaccc agttcataac gcatgctgtc gtaagactga ccgtcaactt tcatgttgtt
    16981 gctcagctgg tagtcatcac cagactggaa cagaccagaa atgctgccgt aaggagtcac
    17041 gtaaccagca tcacccagtt tgaagtcgta accggctttc aaaccaaagc cccaagcgtc
    17101 ggagttggtg ctaccgtcaa cgtaagtacc gttgctcatg gttgcagaca ggtcgttgtt
    17161 gaagtgagag tagctcaagc taccatcaac aaagacgttg ttcgcgaagt gagcagaaga
    17221 gtagatgtag gcagtctggc tgtcttgatc cacctgacca gaacggtcat tcatgtcacc
    17281 tttagcgaaa cctgcagccg caccgacgat ccacttagcg ttgttaccgt caattttggt
    17341 atcaacaccg accatgatgc cgttaacatc ctgatcatag ttgatggtgc cgttgtcgcc
    17401 gttgaagtta ccaccgaagt agcttaccca tgcgccgccg ttatcagcca ggccatgacg
    17461 agagttggtc agacgagtac caacggtgtc ttgttccagg ttccagatat tggtgttcgc
    17521 agacgggatg ctcagcgcca tgttagcgta gtcggtcagc tccatctgtt gcagaacaac
    17581 ggtgttacca cgctgctcag cctgataggt gtatgcaccc aggtcagctt tgttagcagc
    17641 agagaaggtc gcgttgctgt ttttgtcgtt aacgtagatc agttcgttac cgttgtagtc
    17701 agcgatagaa ccagcaccag tagcgttgtc gatacgaact ttgtagttac cggttgcagc
    17761 tgcaacatgg tcaccgttgt tatcaacttc gttctgacca gcttcgatag aagcagtgtt
    17821 gtcgttatcg ccgttaccgt taatggtcag gtgaccatca gagttcattg cgattaaacc
    17881 ataaccgtag tttgacttgg tagtatcgtt ggtacggtcg ttggtcaggt cggcatcaag
    17941 tacgaagcgg ttgctttcga tgttgaacac accagcgtga atgttaccac tggtatcagt
    18001 agtgctggtc aggttcagag tatcaacctg cagagcattc gccgcagtgt taccgacatt
    18061 cagaataccg tcgttagtca ctttgatgtt gttggcatac agagcagcat cacctacagc
    18121 ccaaccagca gaaacttcat cagaaatggt tacggtactg ttgtcgacag tcagtgaatc
    18181 agttgcaaca tgaccatctt cgttgatgtt cagcgcagaa agacctgtca gagagataga
    18241 gtcagaaatc agcgttgaat cagcaacatt aaccgttgaa ccactgttga tgctcagagt
    18301 atcaatcaga gaagttttgg tggtatccca ctgtgaaccg gtgttcaggg tcacattgaa
    18361 cagaccgctc tggtaaactt cgttaccgat gatgtgatca aattcgctgt attcagagtc
    18421 gctgttttct acaccaacag ttgatgaagg ccacaggctg ttagctgcga tgtcgatcag
    18481 agttgcagtg gcttcagtat tgattccata tgtataggag tcatttacac catcgtcatc
    18541 agtatcaaca ccaatctgat gcgcagacat tgccgcaccg acccacttgc tgccattgtt
    18601 cagggtcaga tccaggcgat cagtaccatc ccaaccgtta gtatctacat caccgtcagc
    18661 atcacgatag ctatcacgac catgcgggaa gaagttctca tcaaagttac ttgagaagaa
    18721 tacatcgccc atcagtgtgg aattatcgaa tgttgcagtg gtttgcatgg cgttatcagc
    18781 agcaggatgt gcaataacag ctaatgcgat atcgtcagcg ttccaacctt taccattata
    18841 atcgctggca ttacctgtat taccgaacca accagttgta ccttcatcag tccatgaacc
    18901 agaggtcacg gttgaatctt taacagtgat ggtgttatta aacacttcgc tgctgtttac
    18961 accagtgctg acagtgttgt cgtcgatatc ttcccactca taaccctgag tcagcgtaat
    19021 acctgctaca tgagagttat tagagatgga cagatcaact tcctgatcca gagtgatggc
    19081 agtaccaatg ttaaatacat ttacaacatg agtgtcagcg gcattattgt aggtgccgtt
    19141 atatgtgtag tgttcgtagt tgtcatcgat cgttgaatta tcaacggtca gcgccagacg
    19201 atcatggtag taatcatccg cacgatcagc acaatcagtt gtcatgcact cggaataaat
    19261 catgccgtga atagtgctgt ttttgattgt caggctattg gcgttggtat tagtagaaat
    19321 accgtcatcc agatagtagg tagaaatcac gccgttaacg gtagcgttat taatgaccgg
    19381 atagatatcg ccgttgtaat aactatcagc ggcatagtta ttccagccta cataaccatc
    19441 atagtaaacg tcatcagcat aggtggcgtc gttgtagtga tggaaagtat tgtaagtttg
    19501 accagaaata tcgttggtcg ctgcattagc ctgagaggtg atagccagag tgcaagccaa
    19561 tgctaattgt gatactacaa gtttcttttt ccaggagtgc atttgtcatc cctcctcagg
    19621 gacgtaataa gttgatccat caattatcaa tgcatagaaa acatattatg tttcgcgttg
    19681 acgattatgc ggcgttatag aggaatggtt caattattct ttcccctaag tccgattgcg
    19741 gacatatata aagcacgtga acacacacac agaaagaaat aacaaaaaca caatatttca
    19801 atacgttaat gaaagcgtta attttaaaac atagaccatg aaaatataaa acaactaaaa
    19861 cagacaaata atgtatcaat aagcaatgaa ctttaagtta ttcacttttt gaaaacgtca
    19921 ccaaattccc aaaaacaata atctatggct ataaataaaa attaaaaata ttttcagtga
    19981 atatgaaatt attgctgctt ctattacaaa gatgtcctgg tctctctttc attctcttct
    20041 tgtcaacatc gcgacaactt tcgtaaaaca tccccaccct gcttcaggta tactatgccc
    20101 cttgattcca caaacatcag gcagaccatg acagacttaa tccaacgccc tcgtcgcctg
    20161 cgcaaaactc ctgcgctgcg cgctatgttt gaagagacaa cacttagcct taacgacctg
    20221 gtgttgccga tctttgttga agaagaaatt gacgactaca aagccgttga agccatgcca
    20281 ggtgtgatgc gcattccaga gaaacatctg gcacgcgaaa ttgaacgcat cgccaacgcc
    20341 ggtattcgtt ccgtgatgac tttcggcatt tcccaccata ccgatgaaac cggcagcgat
    20401 gcctggcggg aagatggact ggtggcgcgt atgtcgcgca tctgcaagca gactgtgcca
    20461 gaaatgatcg tcatgtcaga cacttgtttc tgtgaataca cttctcacgg tcactgcggt
    20521 gtgctgtgcg agcatggcgt cgacaacgac gcgactctgg aaaatttagg caagcaagcc
    20581 gtggttgcag ctgctgcagg cgcagacttc atcgcccctt ccgccgcgat ggacggccag
    20641 gtacaggcaa ttcgccatgc gctggacgct gcgggcttta aagatacggc gattatgtcg
    20701 tattcgacca agttcgcctc ctccttttat ggcccgttcc gtgaagctgc cggaagcgca
    20761 ttaaaaggcg accgcaaaag ctatcagatg aacccaatga accgtcgtga ggcgattcgt
    20821 gagtcactgc tggatgaagc ccagggcgca gactgcctga tggtgaaacc agctggagcg
    20881 tacctcgaca tcgtgcgtga gctgcgtgaa cgtactgaat tgccgattgg cgcgtatcag
    20941 gtgagcggtg agtatgcgat gattaagttc gccgcgctgg cgggtgctat agatgaagag
    21001 aaagtcgtgc tcgaaagctt aggttcgatt aagcgtgcgg gtgcggatct gattttcagc
    21061 tactttgcgc tggatttggc tgagaagaag attctgcgtt aatgttcatt atgccgggtt
    21121 atccaacccg gcatttcccc cttaccccgc ccgataaaac ggtttatccc caaggatcgt
    21181 cgcccgatgc ataatccgcc gctgtggcag gtaatccgca ttggcatagt gctgagtcac
    21241 gcgattatcc caaatcgcaa tatcattcgg ttgccagcgc cagcgcacct gaaactccgg
    21301 tttggtgata tgggcaaaca aaaaacttaa caaggcttcg ctctctttct cgctgacatc
    21361 aacaattcgc gtagtaaagc cttcattcac aaacagcgcc tgtttaccgc tcaccggatg
    21421 cgttcgcacc accggatgta gcaacggcgg atttttcgcg accgcctcgc gccagcgttg
    21481 atgctcctcc tcggttttgc gatatttgta ttccgggaac gatttacgga aatcatgctc
    21541 cgcacgcaac ccgctcagca gctggcggaa gggaacagag agcgcctcat aggccgcaat
    21601 accgctggtc cagagcgtat cgccgccggt cgagggtaac tcttttgccg ccagaatcgc
    21661 ccctgcgggt ggcgtttcaa taaatgtcac atcggtatgc cagttgtcgt tatccggcgg
    21721 attatcgtta tgagtatcca gcacgatgat ctcgtcaacc ccttcggcat gagggtaaac
    21781 aggatgaata tgcagctcgc caaaacgttg ggccagcgcg cgttgctgca gcggcgtaat
    21841 ggcctgttca cgcagaaaca ccacctgatg acgcagtacc gcatggtaaa gctgttcaaa
    21901 ctggttatca cttaacgggc gcgtcaggtc ggcacccgaa atttgtgcgc cgatatacgg
    21961 ccccagcgag gtaatgctca gacgttcact cattgtactt ctccatgcca gggcgtcagg
    22021 cggcgctgta acgcgcgcag acccagttct aaaagaaagg cgataatcgc aatcaccgcg
    22081 atccccgcca gcaccacgtc agtcgcgaga aattcacccg ctgactggac cataaaaccc
    22141 aaaccgcgcg tcgcggcaat cagctccgcc gccaccaggg tagaccagcc cacacccaga
    22201 ccaatgcgta atccggtgag aatttccggc agcgcaccgg gtaaaataac aaaccacagc
    22261 acctgcgcac ggctggcacc cagcgactgg gcggcacgaa tgcgaacctg ctgcgcgctt
    22321 ttcacccccg ccagcgccga catcgccaca ggtgcaaaaa tcgctaaata gatcagtaag
    22381 atcttcgagg tttcaccaat accaaaccag atcaccatca gcggcaaata cgccagcggc
    22441 ggcaccgggc gatacaattc aattaccgga tccagaatgc cgcgcaccgt cgggcttagt
    22501 cccatggcga tcccgaccgg aatgccgatc aacaccgccg caagtagcgc cagcacaatg
    22561 cgcgtcaggc tggctgccag atgctgccac aaggtggcgt ccataaatcc ttgaggtccg
    22621 gcaatagtaa gcagttttgc cagtacctgt tgcggcggcg gtaaaaacag cgggctaatc
    22681 agttgcagcg ccgccactgc ccaccataca gtgagtaaaa tcgctaacgt gccaatgctt
    22741 aaggtcacct gacgcgagag cggccagcgc catttcagcc gacgcgaatg cagtttttca
    22801 ttaatgagca cactcatgag aatgcctccc gttgctcaaa tacgcggctt aaaacatatt
    22861 cgcgcatggc gatgaattgt ggatcggact tgatgctgcg gctcggttcc ccggcaacaa
    22921 agcggcgggc gaagttgagc ggcagccgct ccagcacgcg gcctgggccg ggtgaaagca
    22981 gaaccagttc agtcgccata aacaccgctt cttctatatc gtgggtaatc aacagcacct
    23041 gcttgcccgt ctcctgccag agtttcagca gcagggtttg catctggtcg cgggtgaagg
    23101 cgtccagcgc accaaacggt tcatcgagta ataacagctg gggatttgcc gccagcgcgc
    23161 gggcaatccc cacccgctga cgttgaccgc cggaaagctg ccagatgtag cgtttttctg
    23221 ccccttccag ccccactttt ttcagcatct ggtgcgcgat ttccagtcgc tgcattttct
    23281 caacacctgc cagttgcagg ccgaacgcca cgttgtcccg tacattgcgc cacggtagca
    23341 gcccttcatt ctgaaaaacc acgccccgct ctgcgcccgg tccctcaatg cacttacccg
    23401 ccagttgaat gctgccatgc tgataaggca caaaaccggc aatcagattc agcagggtgg
    23461 ttttaccgca gccggacggc cccagcacca ccagtagctc acagctttcc agcgtcaggt
    23521 tgatatcttc cagtgccggt ttgccgccat aatcggcgta aagatgagag atttgcagca
    23581 tctgcgcctc cttttattgc acgaagcgcg aggtcacgta ctggctgtaa tcattcgcta
    23641 cagccgggac tttgccctgc tctttcaaaa actgcgcggt gtcgatgatc gctttgttca
    23701 ccggtccggt cagttctgcc gtttgctgct gcggcgtcag ataggtattc cccttcacca
    23761 gccccggaac gtcaccttca ggtacgccgc ttaaacgtgc cagtttgctg atgttttccg
    23821 gctgtttcag ccacgcgtct ggattagcaa tgtacggttg ttgagcatcg atggcgcttt
    23881 tagcgaacgc tttcacgacc tctggatgtt tctcggcaaa atctttgcgc accacccaga
    23941 cgtccagtgt cggcgcgccc cactgcccga cctgttcaga atcggtcagc accttgccgt
    24001 ctttttccag ggcgttaacc gccggtgccc agacataagc accatcaatg tctccccgtt
    24061 gccatgcggc gataatcgcg ggcggctgca ggttcactat ctccacttgt ccgggtttta
    24121 taccccagtg tttcagcgcc gccagcaggc tgtagtgggt ggtggagata aacggtacgg
    24181 cgatgcgttt gccaatgaga tcttccggtt tgctgatagt tttctttacc accagcgctt
    24241 cggagttacc cagttttgac gccagtaaga agacttcaat cggcacctgt tggctggctg
    24301 ccaccgctaa tgggctggaa ccgaggttac cgatttggac atcgcctgaa gccagcgccc
    24361 gcacgatgct ggctccgctg tcaaacttac gccagtccac cgtcgccccg ctctctttag
    24421 caaaggtgtt atcagcctga gccactttcg ccggttcagc ggaggtttga taggccacgg
    24481 tgacgttcac cgcctgtgcc tgaaaagcga tgaatgccag tgcggcaaga agtgtgttac
    24541 gcgatgaaat tgccatgatt gtctgctccc ctgtcttgtt ataggagcag tattcaggaa
    24601 taaaaacatt cattaaaaga attagtcgtt atcgcacaga tgattttatt cttagcaaaa
    24661 aagcggcaaa ggaaagatta aatatgcaat tacacccact ctgacaattc agtaagttac
    24721 tgcacttcat ttttattaat gcataaacaa gcagaacata atgtctttta ttttattaac
    24781 agcaatgcat ataatgaaat cattatattc acattgcata tataactgta tcattctcaa
    24841 tcaaccacat aatacattca cataatgtat ataatccata tttgtgccta gaacatattt
    24901 ttaatatgta attaattgac aaactaaatc acatctaaat acaatatata aaacaaaata
    24961 actatacaaa ccaaacacac aaacacttaa catatgaact acataaaaga ttacgcagtc
    25021 gtatccccgt acttgaaagc cccttatttt tcagctatca gtaattttta acacttaaaa
    25081 attagcatct gtctgtgcca gagattacgt ctctgcgggc atcgttgtgc ttgtagtata
    25141 ataaataaaa tggagtgtca ttaagtggat aaggttttag attcagccat cctttcttcg
    25201 gcaaataaaa gaaagggtat ccttgctatt ggcgcacatc ctgacgatat agaattgggc
    25261 tgtggcgcgt cgcttgctcg tcttgcgcaa aaaggaattt atatcgcagc cgtggtgatg
    25321 actaccggca actctggcac agatggaata atcgatcgcc atgaagaatc gcgcaacgcc
    25381 ctaaagatat tagggtgcca ccaaactatt catcttaatt ttgctgacac ccgcgcgcat
    25441 ttgcagctca atgatatgat ttccgccctg gaagacatca ttaaaaatca aattccttct
    25501 gatgttgaaa tcatgcgggt atataccatg catgatgccg accgccatca ggatcatctt
    25561 gctgtttatc aagcttcaat ggttgcctgc cgcactattc cacaaattct cggctacgaa
    25621 accccgagta cctggctttc atttatgcct caggtttttg aatccgttaa agaagaatat
    25681 ttcacagtca aacttgctgc attaaaaaaa cataaaagcc aggaacgacg cgattatatg
    25741 cgtcatgatc gcctgcgtgc agttgcacaa tttcgcgggc aacaggtcaa tagcgatctg
    25801 ggtgaaggct ttgtaattca taaaatgatt ctttgagcaa attgcatgaa aacctggata
    25861 tttatatgta tggccgtagc aatattgcta tggttcctga gtacgttaag gcgtaagccc
    25921 agcccaaaaa aaggttgtat tgacgccatt atacctgcgt ataacgaagg cccgtgtctg
    25981 gcacagtcac tggataatct actgcgtaac ccttattttt gccgggtaat ttgcgttaac
    26041 gacggctcca cggacaatac cgaagcggtc atggcggaag tcaaacgcaa atggggcgac
    26101 cgctttattg ccgtcacgca aaaaaatacc ggtaaaggtg gtgcgctgat gaatggcctc
    26161 aattacgcca cctgcgacca ggttttttta agtgatgccg acacctatgt tccgcccgat
    26221 caagacggaa tgggctatat gctggcagaa atagagcgcg gtgctgatgc cgtaggcggc
    26281 attccctcta ctgcgttgaa aggcgcgggt ctgttaccgc acatccgcgc gaccgtaaag
    26341 ttgccgatga ttgttatgaa gcgcacgcta cagcagctcc tgggcggcgc accgtttatt
    26401 atcagcggtg cctgcgggat gttccgtact gatgtattgc gtaagttcgg tttctctgat
    26461 cgtactaaag tcgaagacct tgatctcacc tggacattgg tggcaaacgg ctatcgtatt
    26521 cggcaggcga atcgctgcat cgtataccca caggaatgca acagcccgcg tgaggaatgg
    26581 cgtcgctggc ggcgttggat tgtgggctac gcggtctgta tgcgcctgca taaaaggctt
    26641 ttatttagcc gcttcggtat cttcagtata tttcctatgc tgttggttgt gctttatggc
    26701 gttgggattt atctcactac ctggtttaat gaattcatca ccaccgggcc gcatggtgtg
    26761 gtgttggcaa tgtttccgct tatctgggtc ggcgtagttt gtgttattgg tgcttttagc
    26821 gcctggtttc atcgttgctg gttgttggtg cctttagcgc cgctttccgt tgtgtatgta
    26881 ttattagctt atgccatctg gattatttat ggacttattg ccttttttac tggacgcgaa
    26941 cctcagcgcg acaaacccac ccgctattcc gcactggtgg aagcgtcaac cgcttattcc
    27001 caaccttctg tcacaggaac tgaaaaactt tctgaagctt aatgttaaag agaaaaatat
    27061 tcagattgca gaccaggtaa ttattgatga aactgcaggt gaagttgtta tcggcgcgaa
    27121 tacccgtatt tgtcacggtg ccgttattca gggtccggta gtgattggcg caaactgcct
    27181 gataggtaat tatgcgttta ttcgtcctgg cacaataatc agcaatggcg taaaaattgg
    27241 ttttgccacc gaaattaaaa atgcggttat tgaagcggaa gcaacgattg gtccgcaatg
    27301 ctttatcgcc gactcggtag ttgcaaacca ggcatatttg ggcgcacaag tacgtaccag
    27361 taatcatcgt ctggatcaac agcccgtgtc tgttcgaact ccagagggaa ttatcgctac
    27421 cgaatgcgat aaattaggtt gttatatcgg gcagcgttca cgccttggcg tacaagttat
    27481 tattttgcct gggcgaatta tttccccgaa cacgcaactt ggcccgcgcg tgattgtaga
    27541 acgtaattta cctaccggaa cttattcact ccgacaagaa cttatccgta caggagatta
    27601 aaatgataaa acggacgtta ttagcggcag caatttttag cgcattgccc gcttatgccg
    27661 ggttaacttc cattaccgcg ggctacgatt ttaccgatta ttctggcgat catggcaacc
    27721 gtaatttagc gtatgctgaa ctggtggcga aagttgaaaa cgcaacgctg ctttttaatc
    27781 tttcccaggg gcgtcgtgat tatgaaactg aacatttcaa tgccactcgc ggtcagggtg
    27841 cggtctggta taaatggaat aactggctga caacccgaac gggtattgcc tttgcggata
    27901 atacgccggt ctttgcccgc caggattttc gtcaggatat taacctggca ctgttgccaa
    27961 aaacgctttt cacgaccggt tatcgctaca cgaaatatta cgatgatgtc gaagtcgatg
    28021 cctggcaagg cggcgtatca ctctatactg gcccggtgat ctccagctac cgctataccc
    28081 attatgactc cagcgatgca ggtggtagtt acagcaatat gatttccgtg cgtctgaatg
    28141 acccgcgcgg cactggttat acgcaactat ggctaagccg cggaacaggc gcttacacct
    28201 atgactggac gccagaaaca cgctacggca gcatgaagag cgtcagtttg caacgtattc
    28261 aaccgctgac tgagcaactt aatctcgggc tgacggcagg taaagtgtgg tacgacaccc
    28321 caactgatga ttataacggt ctgcaatttg cagcccatct gacctggaaa ttctgattgc
    28381 ttctgccgcc cgctatccgg ggcggccttc cctgtcgatt aggcccctct ttactttttg
    28441 ttatccgact acattcaccg gataaatttt attctccagt gttatatact ataggggggt
    28501 atgcattgac atatagcata cccccctata gtatattgcg tgcagatgat gaggtgcgaa
    28561 atgcccagta ctccggaaga gaagaaaaag gtccttactc gagttcgtcg tattcggggg
    28621 cagattgatg ctctggaacg gtcgctggag ggtgatgccg aatgccgtgc catactccaa
    28681 cagatcgctg ccgttcgggg cgcggctaac gggctgatgg cagaagtgct tgaaagccat
    28741 atccgggaaa cgtttgaccg aaatgactgc tacagccgcg aagtcagcca atccgttgac
    28801 gacactattg aactggttcg tgcctatctt aaatagctga atctattacc atattgagga
    28861 agagcgagag atgaaatcac gtgctgccgt tgcatttgct cccggtaaac cgctggaaat
    28921 cgttgaaatt gacgttgcac caccgaaaaa aggtgaagtg ctgattaaag tcacccatac
    28981 cggcgtttgc cataccgacg catttaccct ctccggcgat gacccggaag gtgtattccc
    29041 ggtagttctc ggtcacgaag gggccggcgt tgtggttgaa gtcggtgaag gcgtaaccag
    29101 cgtcaaacct ggcgaccatg tgatcccgct ttacaccgca gagtgcggcg agtgtgagtt
    29161 ctgtcgttcc ggcaaaacca acctctgtgt tgcggttcgc gaaacccagg gtaaaggcct
    29221 gatgccagac ggcaccaccc gtttttctta caacgggcag ccgctttatc actacatggg
    29281 atgctctaca ttcagtgaat acaccgtggt cgcggaagtg tctctggcca aaattaatcc
    29341 agaagcaaac cacgaacacg tctgcctgct gggctgtggc gtgaccaccg gtattggcgc
    29401 ggtacacaac acggctaaag tccagccagg tgattctgtt gccgtatttg gtcttggcgc
    29461 gattggtctg gcagtggttc agggcgcgcg tcaggcgaaa gcgggacgga ttatcgctat
    29521 tgataccaac ccgaagaaat tcgatcttgc gcgtcgattc ggtgctaccg actgcattaa
    29581 cccgaatgac tacgacaaac cgattaaaga tgtcctgctg gatatcaaca aatggggtat
    29641 cgaccatacc tttgaatgca tcggtaacgt caacgtgatg cgtgcggcgc tggaaagtgc
    29701 gcaccgcggc tggggtcagt cggtgatcat cggggtagct ggttctggtc aggaaatctc
    29761 cacccgtcca ttccagttgg tcaccggtcg cgtatggaaa ggttccgcgt ttggcggcgt
    29821 gaaaggtcgt tcccagttac cgggtatggt tgaagatgcg atgaaaggcg atatcgatct
    29881 ggaaccgttt gtcacgcata ccatgagcct ggatgaaatt aatgacgcct tcgacctgat
    29941 gcatgaaggc aaatccattc gaaccgtaat tcgttactga tttcccgcag gttttacccc
    30001 gtccagttca ggcggggttc ttaatactct ccccgggcag ccgtccgggg gattaaccgt
    30061 gagataatga ctgatggaac tcattgaaaa acatgccagc tttggcggct ggcaaaatgt
    30121 gtatcggcat tattcccaat cactgaaatg tgaaatgaat gtcggcgtct atctcccacc
    30181 aaaagccgca agtgaaaaat tgccggtgtt gtactggctt tcgggcctga cctgcaacga
    30241 gcagaatttc attactaaat cagggatgca acgttacgcg gcggagcaca acattattgt
    30301 tgttgcgcca gacaccagtc cgcgaggcag tcatgtcgca gatgccgacc gttacgatct
    30361 cgggcaaggt gccgggtttt acctgaacgc aacgcaagcg ccgtggaatg aacattacaa
    30421 aatgtatgac tatatccgca acgaactgcc ggatttagtg atgcaacatt ttccggcaac
    30481 ggccagaaag tctatctcag gtcattctat gggcgggctg ggcgcactgg tgctggcgtt
    30541 acgtaatcca gatgaatatg tcagcgtctc ggcgttttcg cccattgtct ccccatcgca
    30601 agtaccgtgg ggacagcaag cctttaccgc atatcttggt gaaaataaag aagcctggtt
    30661 ggattacgat ccggtgagtc ttatttcaca aggtcaacgc attgcggaaa taatggttga
    30721 tcaggggttg agtgatgatt tttacgcaga acagctgcgg actccaaatc ttgaaaagat
    30781 ctgccaggag atgaatatca aaacgttaat ccgttatcac gagggttatg atcacagcta
    30841 ttattttgtc tccagtttta ttggcgagca tattgcctac cacgccaata aactgaatat
    30901 gcgttaataa tagtgcacga ctgccggatg cggcgtgaac gccttatccg cctacatttc
    30961 gcccgcaggc cgtaggcatg ataaaacgcg catagcgtcg catcaggcat ctgcgcacaa
    31021 cttatctatg tttaccacat caaatcatca ggcactttga agtcggcata cgggtcgtct
    31081 tcatcctgct cttcggcgct gagcgcgctg tgcaacacaa tactgctggc atcacgctga
    31141 gcaattttat cggcaacgct ggcgggaata atggcgtatt caccttcgct attgttatcg
    31201 atcaacaagc gggcaatcgc cagacgacca ttaattaact gcgcttgcgt gagcttatcg
    31261 acaaagattt tcttaatcag attaccgtca gtgaagttaa aaccaatatc gccattagca
    31321 atggtgattc ggttcatttc aatgagctgc ttcacctggg ctttatattc tttcgccaac
    31381 gccgcttgtt tttgctgctc gctaagctgt ttatcacgct caagctgtgc ctttttgttt
    31441 tcttctaccg ccgcccgagc ttcacgcgcc tgaacacgcg attttttcgc cgttctctcg
    31501 accttcgccg cttttttgct ggtgactaat cctgctttga gcaactgctc ttgtaaggta
    31561 agttttgcca tctttgtttc caaacccgct gaataatttt tagcgagtat acctgtaaac
    31621 gccgtaggtg tattgggggc aagtcccttt cgcccctact taataaggcg cgtcaggcat
    31681 cggcgcacgg ctgcattcgt gatctccggc tcatcaaaat aaacactgcc aggccagcaa
    31741 caagaatacc cggcgcagaa gcggccatta cgccaaccgt gccagtgcct aatgccagca
    31801 ttttcccggc cagtaacgga ccgctcatag cccccagacg ccctaccgcc acggctgttc
    31861 ccacacctgt tgcgcggatc tgcgaactgt aaaacaacgg tgccagggca tacaaaacgc
    31921 tttgcccacc tgtcgcaaac aaccccgcga cgaatcccgc cagcagcata ccgttaaatg
    31981 acgacactgt tcccagcgcc agcagcgaag ctaacatgcc gctataaatc agtagcgaca
    32041 tggttactgg acgcagctta tccatcaatg cgcccaacat taacgtcccg cttgccgccc
    32101 ccatttgcag tgcaaacatc acccctgccg cctgcgatgg ctggaatcct tgctccacca
    32161 aaagcagcgg cagccagttg atcaacatgt agaccaccag cagagtgaag aaataacaca
    32221 accacagcag cagcgtcgcg gttgccgttt ctggcgcaaa taacgcacgc agtggaggcg
    32281 cggcctgttt ttcgccagcg aaaaccgccg actccggcag ccagcgcatt aatagtggca
    32341 ccagaatcaa cggcactaca ccaccgaccc aaaacaccgt ttgccaggct aagtttgccc
    32401 ccgtgaaacc cagtgtcgcc gccagcgccg cgccaatggg aacaccgcaa tacatcaggc
    32461 tcactgccgt cccacgaaaa cgtggacccg cggcttcaga cgtcagggcg ataagattcg
    32521 gcaacgccgc ccccagcccg acaccggtca tcagccgcgc aaagaccagt gaggggaaat
    32581 cccaggcaat cgccgttgcc agtgagaaca aaccaaacag cgcaactgag ctaattaaaa
    32641 tacgcttacg accataacgg tccgccagca ttccgccaac cagcgcgccg ggtagcaaac
    32701 cgagtattcc ggcgctgaat atccagccca tttgcatttt atcgagtgcg aaagcctggg
    32761 cgatgccacc cgccgcaatg ccagccgcct gaagatccag tccttccatc agagcgacca
    32821 aaaaacaaag cccgatggtc agcatcaggc gggatgaaga tgatgaaggg atacgagtcg
    32881 acataagatg aacctcgttg cgagagcagg tgaatcgcgg ctgatacatt gccagccgcg
    32941 taatcagaac catttaatga ctgcctacag gcgaagaaaa tgtaggccga acaaggcgtt
    33001 tacgccgcat ccggcagtcg tgctgggatg ggcatacaaa atgcacgttc tcggcagtca
    33061 tcattatttg ttgttgcgca gatccagcgc cacgtcgacg atcatatcct cctggccgcc
    33121 aaccatccgg cgtttgccca gctcaacgag aatatccacc gcgcttaagc cataacgcgc
    33181 cgccgccgtt tcacagtgac gcaggaagct cgagtaaaca ccagcgtatc ccagcgccag
    33241 cgtttcgcga tcgactcgta ccggtcgatc ctgcaacgga cgcaccaggt cgtcagcggc
    33301 atccattaac gcatagagat cggtcccatg ctgccaaccc agtttatccg cggcggcaat
    33361 aaaaacttcc agcggcgcgt tacctgcgcc cgcgcccatt cccgcgaggc tggcgtcgat
    33421 tcggtcgcag ccctcttcca ccgccgcgat agagttcgcc acgccaagac tcaggttatg
    33481 gtgagcgtgc atgccggttt gcgtttctgg tttcagcact gctttcaggg cgcggaaacg
    33541 gtcacggata tcgctcatgt tcatcgcacc gccagaatcc accacataaa tacaggtcgc
    33601 accgtagcct tccatcagct ttgcctgctt tgcgagattc tccggcgtgg tcatatggct
    33661 catcatcaga aaaccaacgg tgtccattcc gagctcgcgg gcatactgaa tatgctgggc
    33721 ggaaacatca gcttcggtac agtgcgttgc cacacgaacc acccgcgcgc cagcctgcca
    33781 ggcatttttc agatcgtgaa tagtgccgat tccaggcagc aacaacgtcg cgattttggc
    33841 gtgcttcacc acatccgccg ccgcttcaat ccattctagg tcgctatgtg cgccgaaacc
    33901 atagttaaag ctggaacctt gcaaaccgtc gccgtgggcc acttcaatcg aatccacgcg
    33961 ggcatcatcc agtgctttgg caatctggcg gacattttcc agcgaatact gatgacgaat
    34021 ggcgtgcata ccgtcacgca acgtgacgtc cgaaatataa agttttttac cgttcatgcc
    34081 gcttctcctg ccttgcgcac cagtgactgg gccatttttt ccgctgtcgc cagcgcactg
    34141 gaagtcataa tgtcgaggtt gcccgcatag gcaggcagat aatgcgctgc gccttcgact
    34201 tccagccaga ccgccgtttt cagcccggag aactgcccca cgcccggcaa attaaccggt
    34261 ttatcctgcg ggataacttc aaactgcacg cgctgtttca ggcgataacc cggtacgtaa
    34321 gcctgcaccg cctcagccat ttcattgatt gaggcttcga tatcatcttg tgaagcttcg
    34381 tcgctcaata catacaccgt gtcacgcatc atcaacggtg gctctgctgg gttaagcacg
    34441 ataattgcct tcccttttgc cgcgccgccc accacttcaa tggcgcgcga agtggtttcc
    34501 gtaaattcat cgatattggc acgcgtgcca ggtccggcag atttactggc gatagaagcg
    34561 ataatttcgg cgtaatgaac acgcgccacg cgtgaaactg ccgccaccat tggaatggtg
    34621 gcctggccgc cgcaggtgac catgttgacg ttcagttgat cgacgttctc ctcgaggtta
    34681 accaccggca cgcagtaagg gccgatggca gcaggcgtca ggtcaattaa gcgaatatcc
    34741 ggtttcgctt cgcgcaaagc ggcatcgttt ttcacatgcg cgcccgcgct ggtcgcatca
    34801 aatacaatgt cgatatcagc aaattcaggc atgttcatca gcccgatcac cccttcatgg
    34861 gtggtggcga cgcccatacg tctggcacgc gccagaccgt cggactgagg atcaatgccg
    34921 accatcaccg ccatctccag atgctgaccg tgacgcaaaa ttttaatcat cagatcggtg
    34981 ccaatgttgc cagaaccgat aatggcgact ttacgcttac tcatgacaga cttccttttg
    35041 gggctgcgct tgaaaatgtc gcagcaactg aacctatgcc ttcgatatgg gcttcaaaac
    35101 gatcgcccgc attcaccgcc accatcggac ctaatgcccc ggtaagaatg atatctccgg
    35161 cgcgcagcgg ttcacccaga ctggccattt tgcgtgccag ccagacggcg gcattgagcg
    35221 gatgtcccag gcattcgctg ccgcgcccgc tggaaacctc ttcgttatta cgcgtcatct
    35281 tcatggcgca gtttttcagg tctaaccccg ccggacgttg cgccggaccg ccgatgacat
    35341 acaccccaca ggaggcgtta tctgccacgg tatcgacaaa ctgaatcgac cagtcgcgaa
    35401 tgcggctccc caccacttcc agcgccggaa gtacccattc aatggcgtta tacaattcgt
    35461 cgaaggtgat atcggttgcg ggtagatcgc ggttcaacac cagtgcaatc tccgcttcaa
    35521 tgcggggttg cagaacacgg gaaaaaggaa tggtttcgtt atcgccataa cacatgtcgg
    35581 caaataatgt cccgaaatcc ggttgatcaa cgcccagttg ttgctgcact ttcggatgcg
    35641 tcagccccac tttgcgcccc accactcgcc gtccctgagc gacgtcatgt tgcacattga
    35701 tgcgctgaat ggcgtaggcc gcttcagcgt tttcaatacc aatcagatcg cgcagcggcg
    35761 caatcgcttc gccctgctct gcggcgcggc gtaaatccgc cgccagttgc tcaagagtat
    35821 gcttcgtcat gaccgttcct taagggcgtg cgaggaaatt cagcaccagt tgattgaaag
    35881 cgtcggcatg ttcccactgc gcccagtgac cacagtcgcg gaagatatgc agttcagaac
    35941 cggcaatgcc ggacagcaga cgcagacccg catccatcgg tacaaagcgg tcgttgcgcc
    36001 cccagacaat cagggtttgc gctttgattt ccgccagacg tgggccaaaa tccgggaact
    36061 gtttcggatt ggcttccagg ctcttaacga agttttccag gtgatcgcgg cgcgacagca
    36121 tattattcag gcgcgcttca aacagggcgt cggtcaaatc gctggtatca aaaacgaaga
    36181 tatccatcat cagcttcagg ttttcgatag tcggctgacg ataaagctga ttcagtcgct
    36241 taataccttc ggttggcatc ggcgtaaaca aactcatacc gcccgtaccg ccgcccatca
    36301 gcaccagttt gccgacgcgc tccggccagt taagagtgaa cgccacagaa ctgtggccgc
    36361 ccatcgagtt gcccagcagg tggattttgg cgatatccag ttgatccacc acgcttttca
    36421 ggattcgcgc attaagatcc gatcgcgaac cactattaac gatcgaatcg ctcttacccc
    36481 aacccggaca atccagcagg atcacccgat agcccgcctc taccagcgga tcgatattgc
    36541 ggctgaagtt cgcccagcca gtagcacccg ggccggaacc atgcagcagg acaacagttt
    36601 cgtcgccttg tccgcagtca ttaaaatgga tgcgcagcgt tttacccgct tcttctacat
    36661 tcagaaaacg gctggtggcg gcttcggttt gtggctgata actcatcatc ttctcctgca
    36721 tattcagttc tctgttctgg cgcttaacga gccaaatccg gcaatccact ccgggattgg
    36781 gcggtaataa cgcccttcgc tacgccagtt gccaaacgca gaaatagcgg caaaagcggc
    36841 gacccaggtt ttgatttcat gtgtcgactt tccggcaatg gcggaaagct cttcgttact
    36901 gacggcatcc agttcctgta tgcgtccctg ctccagcaaa gtcatgaact ggttatccca
    36961 aatcgggttg aggggatgca gcgttctctg atcctcaaca aacttctcag cggcgctaat
    37021 cacccgttgc tgacgcaatt cgcgctcact ggcgggtaaa tctttcccgc tccccaacag
    37081 acggtcgcgc atatgggcat cggctttcgc cagttctggc accggcggct gatgggaaag
    37141 cccaccggaa cccaggaaca gcacgcgttt attgagagta ctggtgaaac gtccaatggc
    37201 ttcacccaac atgcgggtac gctggaaacc gggcaacggc gtggcgacac cgttgatgaa
    37261 cacaggcaaa accggcacct tatccagccc gccgagcagg aactccagcg gctgggcgaa
    37321 cccgtggtcc acctgcatac agtaagaaac ggcaagatcg atcccgctct tcatgacggc
    37381 atgcgcacag gcctccgcca gctccacagg tacgggcagc tctcctgccg cactaccgaa
    37441 atcaccaatt gccgtcgccc caacgcctaa acagaacggt ggcatcacgt catagaaaaa
    37501 gccgttgtag tgatctggcg caaacagcac caccagttca ggggagaaag ccgcaatacg
    37561 ctcgcgggcg ctggcaatca cgccattgac ctcatcgagc acctcttgcg ccgggtcgac
    37621 ataccccacc agcggcgagt gggaaagaca gtgaagataa gcgtgcatat caggctacct
    37681 tttcgacaga aacatcggca tcagggcggg tcagcgtcat caccgacgcc agtttattca
    37741 gggtcttgcc cagggtttgc ggaatggcgg tggcggcaac aaagcgatcc gggcgcatca
    37801 ccaccagcga agcattgtgt tgcgcgaacc agctacgcag gcgaccttgc gtatcgccca
    37861 cgcgtagtac gccgtcgtgg ttatcctgtg cggtatgaat ttgcacttcc ggcaccacct
    37921 gaatgaagcg tgtgcccaac gcgcgccact gctggatttg ctcgtcgctc atcccccaca
    37981 gtggattgca tccccagcca attaccgcga agttcgcgcc gatcgcgtta tcgagcagcg
    38041 tcacgtcgcc gttttccagc gtgactttcg gctgaataaa catcttgccg accggagagt
    38101 gcttcgcttc gccctcacgc accagcgcac cgccgtaata ttgcggcatc ggcttgaagc
    38161 gcatttcgag gaagtagcgt tttactggcg gcagataatt caacagccag gaaacgccgt
    38221 cacgtaacgt accctgccag cgtttcggcg gagccagcac gttgcccgcc gtcacggaca
    38281 ggtcaatcat cgctttggcg tgatcgcgac gttcttgttg ataggtatcg agcagcgcat
    38341 cacgggcttt cccctggata accaacgcca gtttccatgc gaggttaaag gcgtcgcgca
    38401 taccactgtt atagccctgc ccctgccaca ccggcatgat gtgcgcggca tcgcccgcca
    38461 gcagtacgcg atcaatacgg aaacgttgcg ccagtcgcgc gttgtgggtg tagacacgct
    38521 ggcgaatcaa ttcaacattg tccggattag gcaacacttt gcttaacagc ttgcgcatat
    38581 tttgcggctc acgcagctgt tcttcggttt ctcccggcat caccataaat tcaaagcgac
    38641 gtaccgcatg aggcagcgcg gcagaaacat acgggcgcac cggatcacaa cacaaataga
    38701 tatggggcgt acttaacgga tcgttggcga tatctaccac aatccactga tttggcgcag
    38761 ttttaccttc aaacggcaca ttcagagtgc gacggacaaa acttgcccca ccatcacagg
    38821 ccaccagcca ctgggctttg accgtttccc gctgcccttc tgccgttttc aggtgcaagg
    38881 tcacttcgtc atcttgctgg ctgaaggcct ccagctcgcg ggaaaacagg cagcgcacat
    38941 tcggaaaacg cgacaatcct tccagcatca ccgcatcgac ctgcggctga ataaaggcgt
    39001 tacggcgcgg ccagccaaat tcatcggtca ttggctgaat atcagcaaaa cagcgacctt
    39061 ttggggtgag aaaacgcatc gcgtgccacg gcgtagtgtg cggcagaaca ttatcgacca
    39121 ggccaaccga ctgcatggtg cgcagcgcct cgtcatcaat accaatcgcc cgcgggtagt
    39181 cgatcaactt atcgagtttc tccaccacca gcacgtcaat gcccatctgg ccgagatagt
    39241 tcgccatcat cagcccgacc ggaccggcac cagcgatcgc cacctgaacg ctatggttaa
    39301 cagcaggctg gatgtcaggg tgttgtattg ccatttcagt acctcacgac tcggacaaaa
    39361 tgccgttgcg cgcacagtac agcgcaactt attttgttaa aaacatgtaa atgatttttt
    39421 attgtgcgct cagtatagga agggtgtttt cggctacaat caaaacatgc ccgaatgtgc
    39481 accaggtgca caacgttgtt ttaactatag aaatgtcaat taatatgcag aacaatgagc
    39541 agacggaata caaaaccgtg cgcggcttaa cccgcggtct aatgttatta aatatgttaa
    39601 ataaacttga cggcggtgcc agcgtcgggc tgctggcgga actcagcggc ctgcatcgca
    39661 ccactgtgcg gcgactgctg gagacgctgc aggaagaggg atatgtccgg cgtagtccct
    39721 ccgatgatag ttttcgtctg accatcaaag tgcggcaatt aagcgaagga tttcgtgacg
    39781 aacagtggat ttctgcactg gcggccccgc tgctgggcga tctgttgcgc gaagtggttt
    39841 ggccaacaga tgtgtccacc ctggatgttg atgcaatggt ggtacgcgaa accacccacc
    39901 gtttcagccg cttatccttt caccgggcaa tggtcgggcg acggttaccg cttctgaaaa
    39961 ccgcctcggg cctgacctgg ctggcctttt gcccggaaca agaacgcaag gaattaatcg
    40021 aaatattagc cacccgtccc ggtgatgact atcaactggc acgggaaccg ttaaagctgg
    40081 aagccattct ggcgcgcgcg cgcaaagagg ggtacggaca gaactaccgc ggctgggatc
    40141 aagaagaaaa gatcgcctct attgccgtac cgctgcgcag tgaacaacgg gtgattggct
    40201 gtctgaatct ggtgtatatg gcgagcgcaa tgaccattga acaggcagcg gaaaagcatc
    40261 ttccggcgct acaacgggtg gcaaaacaga tcgaagaagg ggttgaatcg caggctattc
    40321 tggtagcggg aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gcgcaaaacc
    40381 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
    40441 ccagtaacgc tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
    40501 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
    40561 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
    40621 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggca
    40681 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
    40741 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
    40801 atcattaact atccgctgga tgaccaggat gccattgctg tggaagcagc ctgcgctaat
    40861 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
    40921 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca tcagcaaatc
    40981 gctctgttag caggtccatt aagttctgtc tcggcacgtc tgcgcctggc gggctggcat
    41041 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
    41101 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
    41161 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
    41221 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcgtgttat
    41281 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
    41341 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
    41401 ctggtgaaaa gaaaaaccac ccttccgccc aatacgcaaa ccgcttctcc ccaggcgttg
    41461 gcagattctt taatgcagct ggcgcgacaa atttcccgac tcgaaagcgg gcagtgagcg
    41521 caacgcaatt aatgtgagtc agctcactca ttaggcaccc caggctttac actttatgcg
    41581 tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag gatacagcta
    41641 tgactataat tacggattca ctggccgtcg tattacaacg tcgtgactgg gaaaaccctg
    41701 gcgttaccca acttaatcgc cttgcggcac atcccccttt cgccagctgg cgtaatagcg
    41761 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
    41821 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
    41881 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
    41941 acaccaacgt gacctatccc attacggtca atccgccttt tgttcccgcg gagaatccga
    42001 cgggttgtta ctcgctcaca tttaatattg atgaatgctg gctacaggaa ggccagacgc
    42061 gaattatttt tgatggtgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg
    42121 gttacggcca ggacagccgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg
    42181 gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc
    42241 aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcac aaaccgacca
    42301 cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgcggtac
    42361 tggaggcgga agttcagatg tacggcgagc tgcgcgacga gctgcgggtg acggtttctt
    42421 tgtggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcgtcggt gaaattatcg
    42481 atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaatgtcgaa aacccgaaac
    42541 tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accaccgacg
    42601 gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg gttgaaaatg
    42661 gtctgctgct gctgaacggc aagccgttgc tgattcgcgg cgttaaccgt cacgagcatc
    42721 atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga
    42781 agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaatcatccg ctgtggtaca
    42841 cgttgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca
    42901 tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg
    42961 taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt aatcatctgg tcgctgggga
    43021 atgaatcggg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc
    43081 cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt
    43141 gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca
    43201 tcaaaaaatg gctttcgctg cctggagaac tgcgcccgct gatcctttgc gaatatgccc
    43261 acgcgatggg taacagtctt ggcggcttcg ctaaatactg gcaggcgttt cgtcagtacc
    43321 cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg
    43381 aaaacggcaa cccgtggtca gcttacggcg gtgattttgg cgatacgcca aacgatcgcc
    43441 agttctgcat gaacggtctg gtctttgccg accgcacgcc gcatccggcg ctgacggaag
    43501 caaaacacca gcagcagttt ttccagttcc gtttatccgg gcgaaccatc gaagtgacca
    43561 gcgaatacct gttccgtcat agcgataacg agctcctgca ctggtcggtg gcgttggatg
    43621 gcaagccgct ggcaagcggt gaaatgcctc tggatgttgc tccacaagat aaacagttga
    43681 ttgaactgcc tgaactaccg cagccggaga gcgccggaca actctggctt acggtacacg
    43741 tagtgcaacc gaacgcgacc gcatggtcag aagccggaca catcagcgcc tggcagcagt
    43801 ggcgtctggc ggaaaacctc agcgtgacac tccccgccgc gccccacgcc atcccgcaac
    43861 tgaccaccag cgaaacggat ttttgcatcg agctgggtaa taaacgttgg caatttaacc
    43921 gccagtcagg ctttctttca cagatgtgga ttggcgatga aaaacaactg ctgacgccgc
    43981 tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc
    44041 gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag
    44101 tggcgttgtt gcagtgcacg gcagatatac ttgccgacgc ggtgctgatt acgaccgctc
    44161 acgcgtggca gcatcaggga aaaaccttat ttattagccg gaaaacctac cggattgatg
    44221 gtagtggtca aatggcgatt accgttgatg ttgaagtaac gagcgataca ccgcatccgg
    44281 cgcggattgg cctgacctgc cagctggcgc aggtcgcaga gcgggtaaac tggctcggat
    44341 tagggccgca agaaaactat cccgaccgcc ttactgccgt ctgttttgac cgctgggatc
    44401 tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg
    44461 ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaatatca
    44521 gtcgctacag ccaacaacaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag
    44581 aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga
    44641 gcccgtcagt gtcggcggaa ttccagctga gcgccggtcg ctaccattac cagttggtct
    44701 ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat
    44761 ccattatgta ctatttaaaa aacacaaact tttggatgtt cggtttattc tttttctttt
    44821 acttttttat catgggagcc tacttcccgt ttttcccgat ttggctacat gacatcaacc
    44881 atatcagcaa aagtgatacg ggtattattt ttgctgctat ttctctgttc tcgctattat
    44941 tccaaccgct gtttggtctg ctttctgaca aactcgggct gcgcaaatac ctgctgtgga
    45001 ttattaccgg catgttagtg atgtttgcgc cgttctttat ttttatcttc gggccactgt
    45061 tacaatacaa cattttagta ggatcgattg ttggtggtat ttatcttggc ttttgtttta
    45121 acgccggtgc gccagcagta gaggcattta tcgagaaagt cagccgtcgc agtaatttcg
    45181 aatttggtcg cgcgcggatg tttggctgtg ttggctgggc gctgtgtgcc tcgattgtcg
    45241 gcatcatgtt caccatcaat aatcagtttg ttttctggct gggttctggc tgtgcactca
    45301 tcctcgccgt tttgctcttt ttcgccaaaa cggatgcgcc ctcttccgcc acggttgcca
    45361 atgcggtagg tgccaaccat tcggcattta gccttaagct ggcgctggaa ctgttcagac
    45421 agccaaaact gtggtttttg tcactgtatg ttattggcgt ttcctgcacc tacgatgttt
    45481 ttgaccaaca gtttgctaat ttctttactt cgttctttgc taccggtgaa cagggtacgc
    45541 gggtatttgg ctacgtaacg acaatgggcg aattacttaa cgcctcgatt atgttctttg
    45601 cgccactgat cattaatcgc atcggtggga aaaacgccct gctgctggct ggcactatta
    45661 tgtctgtacg tattattggc tcatcgttcg ccacctcagc gctggaagtg gttattctga
    45721 aaacgctgca tatgtttgaa gtaccgttcc tgctggtggg ctgctttaaa tatattacca
    45781 gccagtttga agtgcgtttt tcagcgacga tttatctggt ctgtttctgc ttctttaagc
    45841 aactggcgat gatttttatg tctgtaccgg cgggcaatat gtatgaaagc atcggtttcc
    45901 agggcgctta tctggtgctg ggtctggtgg cgttgggctt caccttaatt tccgtgttca
    45961 cgcttagcgg ccccggtccg ctttctctac tgcgtcgtca ggtgaatgaa gtcgcttaag
    46021 caatcaatgt cggatgcggc gcgagcgcct tatccgacca acatatcagg acggatgatc
    46081 gcaatgaaca tgccaatgac cgaaagaata aaagcaggca agctatttac cgatatgtgc
    46141 gaaggcttac cggaaaaaag acttcgtggg aaaacgttaa tgtatgagtt taatcactcg
    46201 catccatcag aagttgaaaa aagagaaagc ctgattaaag aaatgtttgc cacggtaggg
    46261 gaaaacgcct gggtagaacc acccgtctat ttctcttacg gttccaacat ccatataggc
    46321 cgcaattttt atgcaaattt caatttaacc attgtagacg actacacggt aacgatcggt
    46381 gataacgtat tgattgcacc caatgttact ctttccgtta cgggacaccc tgtacaccat
    46441 gaattgagaa aaaacggtga gatgtactct tttccgataa cgattggcaa taacgtctgg
    46501 atcggaagtc atgtggttat taatccaggc gtcaccatcg gggataattc tgttattggc
    46561 gcgggtagtg tcgtcacaaa agacattcca ccaaacgtcg tggctgctgg cgttccttgt
    46621 cgagttattc gcgaaataaa cgaccgggat aagcactatt attacaaaga ttataaagtt
    46681 gagccttcag tttaaataag cactgaaata aatgtcggat gcgccattgg gcacatccga
    46741 caataattca ctcttccatt atctggcgaa acaagcgtcg catcatgcct ctttgaccca
    46801 cagctgcgga aaacgtgctg gtgcaaaacg cagggttatg atcatcagcc caacgacgca
    46861 cagcgcatga aacgcccagt ccatcaagta attgccactg atactacgca gcacgccaga
    46921 aaaccaggga gcaagcccgg cgatgataaa accgatcccc tgcataaacg ccaccagttt
    46981 gccagcaata gccggttgcg cagagtgatc gagcgccagc agcaaacaga acggaaatgc
    47041 gccgcccaga cctaatccac acaccattgc ccacaatacc ggcaattgca gcggcagcca
    47101 gataaagccg cagaacccca ccagttgtaa cgccagcgcc agcatcaaaa gtttccgccg
    47161 atcatgatgg cgagccatag caggcatcag caatgcccct gctgcttgcc caagcgtcat
    47221 caatgccagc aaggaaccgc tgtactgcgc gctggcacca atctcaatat agaaagcggg
    47281 taaccaggca atcaggctgg cgtaaccgcc gttaatcaga ccgaagtaaa cacccagcgt
    47341 ccaggcgcgg ggagtaaata ccacgcgaac cggagtggtt gttgtcttgt gagaagaggc
    47401 gacctcgcgg gcgctttgcc accaccaggc aaagagcgca acaacggcag gcagcgccca
    47461 ccaggcgagt gtttgatacc aggtttcgct atgttgaact aaccagggcg tcagggcagc
    47521 accaaggcca ccgccgccca tcagagccgc ggaccacagc cccatcacca gtggcgtacg
    47581 gtgctgaaac cgccgcttaa ttaccgaagg catcactgcc tgaatgatgc cgatccccac
    47641 tccaccaagc agtgcgctgc taagcagcag cgcactttgg gggtaaagct cacgcatcaa
    47701 tgcaccgacg gcaatcagta acaaactgat ggcgacgctg cgacgttcgc tgacatgctg
    47761 atgaagccag cttccggcca gcgccagccc gcccatggta accaccggca gagcggtcaa
    47821 cagggcagcc acgctaaagc tcattccgct tgcctggcgc aattgcggta gcagtggccc
    47881 gacggaggtg agcagtggtc gcatattaag accaatcagc accagaacca gcagcatcgt
    47941 gaacgtcttt tgatggttgc gcatggctgt cagaacggtt tggtcggcag atatttacca
    48001 tctaaggtga taaccgcacg ttcgccaccc tccgggtccg ccactttctt aacgtcgagt
    48061 ttgaagttaa tcgcgctgat aatgccatcg ccaaacttct catgaaccaa cgctttcagg
    48121 gttgtgccgt acacctgcaa catttcatag aaacgataca tcgttgggtc ggttggaata
    48181 cggtcatcaa tgcagccacg cagtggaatc atctgcaaca gtagaatagc gtcttcgtcg
    48241 agatccagct tcgctccgac caggcgggcg gcgtcggcag gaagcgcctg ctgacccagc
    48301 aaagccgcgg ttacgaaggc ttctgccaga ccggtgccgt cggcaatctc ggcaaatgag
    48361 agatcttttt tagctttgct gagcaaaatg gcatcggcaa gatcaagacg aatattgcgg
    48421 ttaatttgcg actgaatcat ggtggatctc ctgatggttt aaaaataagg ttacgctgcg
    48481 gtcggttggc gtagcggtat ggcacaaacg cgaggattag cggccagtgg cacaaactgg
    48541 cgggttgcgc cgtcaaaagc tgcgatgctg ccgctttcaa tgtcgtagac ccagccgtgc
    48601 agggcgatcc gcccctcttc gagcgccagg cgcaccgatg gatgagtttg caaattagcc
    48661 aactgagcaa tgacgttttc acgtaccatc gccgcagctt ttgacggtaa atcgggatgc
    48721 gggcgcgcct cattaacgac gcgggctgaa tcggcataac gcagccagtg ggagacggca
    48781 ggcatatggt ccatgcactg acagctggca atggaggtca tcgcgccaca gttggaatga
    48841 ccgcaaatca caatgtcaga tacccgaagc gcagcgacgg cgtactccac cgaagcagaa
    48901 acgccaccgg gttccggccc gtaagaaggg acgatattgc ccgcgttgcg aataacgaac
    48961 agatcgccag gctcacgttg cgtcaccagc tcagggacca gacggctgtc ggagcaggag
    49021 ataaaaagtg tgcgcgggct ttgctgtgtc gccagctgtt taaacaaggc ttcccgcttc
    49081 ggaaatgcct cgcgctggaa tttaaggaat ccatcaataa tctctttcac cgttaacctc
    49141 tgtctgtctc tggaatgaga ggcagactac tcgccgattg tcataaggta aaagactcat
    49201 ttatgatgag ttctattgga tttacttata ggttggcgaa tgctctctcg acatatcaat
    49261 tattttcttg ccgtggctga acatggcagc ttcacccgtg cagccagtgc gttgcacgtc
    49321 tcccagccag cactttccca gcagattcgc cagttagagg aaagtttagg cgtgccgctg
    49381 tttgaccgta gtgggcgaac gattcgtctc actgatgcag gagaagtctg gcgacagtac
    49441 gccagccggg cgttacagga actgggggcg ggtaaacggg cgattcatga tgttgccgat
    49501 ctgacgcgag gatcgctgcg tatcgccgtc actcccacct ttacaagcta ctttatcggc
    49561 cctttaatgg cggatttcta tgcgcgctac cccggcatca cgctccagct acaggaaatg
    49621 tcgcaggaga aaatcgaaga tctgctttgt cgcgacgagt tggacgttgg gattgccttc
    49681 acgcctgtgc attcgccgga gctggaggca attcctttac tgacagaaag tttagcgtta
    49741 gtcgtggcgc aacatcatcc gctggctgcc tgtgaacagg tggcgttgag tcgcttgcat
    49801 gatgaaaaac tggtcctgct cagcgcggaa tttgccacca gagagcaaat tgaccactac
    49861 tgcgagaaag cggggctaca tccacaggtg gtcattgagg cgaactcaat tagcgcggtt
    49921 ctggagctga ttcgccgcac ttccctttcc acattgttgc cagcagcgat tgccacacaa
    49981 catgacgggc ttaaagctat atcccttgcc ccaccgttac tggagagaac ggcggttttg
    50041 ttgcggcgga aaaatagctg gcagacagcc gccgcgaagg catttttaca catggcgttg
    50101 gaagaatgtg cggatgttgg cgggcatgaa tcacggtagc cactgacaca ggtcagtggc
    50161 tcttcaacgc agaggaaatt accgtttgta atcgatggct tctggctgct ccagatatac
    50221 ggtggtttgt gctggttggg tgctggcaat caccttaccg ccacgtaccg aatagcgcac
    50281 cggaacctga cggcgcagcg cgtcaaaccc attttcagcc ggcaggataa tcaggttggc
    50341 gctgtttccg gcggcaatgc cgtaatcctg caaattcaac gtcctggcgc tatggtgggt
    50401 gattaaattc aggccatcgt taatctgccc gtagcccatc agctggcaaa catgcagccc
    50461 catatgcagc acttgcagca tattcgccgt tcccagcgga taccacggat cgaagacatc
    50521 atcgtgacca aagcagacgt taatgccgga ctccagcatc tctttaacgc gcgtgatgcc
    50581 gcgacgtttt gggtacgtat cgaaacgtcc ttgtagatga atattgacca acgggttggc
    50641 gacaaagtta ataccggaca ttttcagcaa gcggaacagg cgtgaggtat acgccccgtt
    50701 ataggagtgc attgccgtgg tgtggctggc ggtgactcgc gcgcccatgc cttcacggtg
    50761 cgccagggct gcaacggttt cgacaaagcg cgactgttcg tcatcaatct catcacagtg
    50821 aacgtcgatg agacggtcgt atttttgcgc cagggcgaag gttttatgca gcgactccac
    50881 gccgtattca cgggtaaatt caaaatgcgg aatcgccccc actacatctg cccctaagcg
    50941 taacgcctct tccagcaacg cttcaccgtt gggatacgac aaaatccctt cctgcgggaa
    51001 ggcgacgatt tgcagatcaa tccacggcgc gacttcctgc ttcacttcca gcattgcttt
    51061 cagcgcagtc agcgttgcat ccgaaacatc gacatgggta cgcacatgct gaatgccgtt
    51121 ggcaatctgc catttcagcg tttgctaggc gcgttgtttc acatcgtcat gggttaataa
    51181 cgctttgcgc tcggcccagc gttcaatgcc ttcaaacagc gtgccggact gattccagtt
    51241 cggttgtccg gcggtttgcg tggtgtccag gtgaatatgt ggctccacaa acggcggtat
    51301 aactaaacct tgttcggcat ccaggctgtt ttcagttacg ggcatcaccc cggattgcgc
    51361 atcaatggcg ctgatttttc cgtccagcag atgaatctgc cacagcccct ctttgccggg
    51421 taaccgggcg ttaataattg tctgtaaagc gttattcgac actgttagcc tccacatgcg
    51481 tcattgcagc cgtcgttttg cgattcaaaa tcgggttaag gatcagatag ctcagcgcgc
    51541 cacctaatac cgcgttgacc ggaacaatac ccggtaacca gtggcctgcg gcaatcccca
    51601 aggcgaccgc cagaatcgcc acccaattga cactcatcat acgcgtggtc gcaaagtgct
    51661 catagcggcg acggttcatc agatagtcag cgatgatcac gccccctacc ggaggaatag
    51721 ctgccgaaag gaaggtcagc cagccgacaa aattgttata cagccatagt gcgcagaccg
    51781 taccgataat accgttgatt accgaaaggg ttttgctcga catcccggta atgttggcga
    51841 aacctaagcc cgacgcatag agcgcgttat cgttggtggt ccagatattc agccccagca
    51901 ccacaatcgc aggcagcagc agaccctgag caatcatcac atcagagata tccgccatcc
    51961 ccagtgccgc cgcgcccgct gcaccgaaaa taaacatcaa cgagttgccg aggaaaaagg
    52021 ccaccatcgc caccagcacc gccagtttgg cattacgacc aaaccggaca aagtcagcgg
    52081 tgagcgtgcc cgcactgata aatgacccca caaccagcgc cagcgcgaca ttgaaatcta
    52141 acggttgtgc gggaacgacc gcttttaatg cgtccaggcc gcccatgccg ttaacggcca
    52201 accacacgga ataaccgcca agacaggcga tagccggaac tgcaatcacc gaaagaaccg
    52261 tcagcgccga aatgccaaag aagacggtga cggtcatcag taaaccggag acggcaatca
    52321 gcaaattaat atccagcccg gttgccttgc ccaccggaat agcaaacatc gccacgccaa
    52381 cgccaaacca gccgacctga gtgccgccca gcagcagtga aggcagccat gagcctttaa
    52441 caccaaacga gaagcgagca agaagatgag tggtcaggcc ggtttttgcg ccaatgtaac
    52501 caagaaatga agtgtaaata ccgaggagaa gattaccgat gagaactgcg aggaagaaat
    52561 catgatagct aagaccggtt ccgagagtgc cgccggtcca catactggcg gaaaagaagg
    52621 ttaatcccag catgacgaac gtcaatgcca ataccccttt ccgcgccgac tgcgggactg
    52681 gcccctggct aaagttgtta tcttgcgaca cgaaattccc cctttttgct gtttgaaacc
    52741 caaaaaatcc gccgcattct attcatctga agataaaaag caatcgtttt cgtggggaaa
    52801 tatatttttt atatgaatga atgcaaaaag gtgggggaaa aattatcggt gtaaaaacag
    52861 tgggtagcca taataagacg cggtaagcgt cgcatcaggc atcggcgcat ggtgtcggat
    52921 gcgccaggcg acgcatccga caataaccta ctcttccatc gcctggcgga tctgatccaa
    52981 cgacgcagga tcatcaatgg tcgtcagatc ccctggatcg cgtccttcgc aaatcgcctg
    53041 gatcgtgcgg cgcagcattt ttccggatcg cgttttcggt aattgcgaga caaaccagac
    53101 gtgcgccggg cgaccaaagt tgccaatctg gctgtccacc agcgccataa tcgccttctc
    53161 ttgcgagtgc gccacctcgc gatcttccag gctgtcgctc tctttcggaa tgacaaacgc
    53221 caccgccacc tgccctttca gcgcatcttt caccccaacc actgccactt cggcaacgcc
    53281 cggatgactg gagatactct cttcaatctc gcgcgtcccc agccgatgcc cggcaacgtt
    53341 aatcacatcg tcagtgcgcc cgagaataaa gtgataaccg tcagcatcgc ggatccccca
    53401 gtcaaaagtg gcgtacaccg gacgggaaaa cagcgaccag tacgtcttca caaagcggtc
    53461 gtcgtcgccc caaatggtct gaatacaccc cggcggcagc ggcccttcca ccaccagcat
    53521 ccctttctcg ttgacgccgc acggttcgcc ggtgacttca ttgagcaact gcacgttata
    53581 gccatacatc ggcacgccgg ggctacccag acgcgtcggc ctgtcgtcca gaccgcgagc
    53641 aatcgccata atcggccagc cggattcggt ctgccagtag ttgtcgatga ccggcacatc
    53701 cagcgtattg ctcacccaac tggcggtcgg ctcgtccagc ggctcccccg ccagatagag
    53761 cacttccagc gacgagagat cgtgtttgcg aatttcagcg gtagggaatt ttttcagcac
    53821 gcgaatggcg gtcggcgctg agaacatccg gctaacctga tatttctcga cgattttcca
    53881 ccacacaccg cagtccggcc aggtcggcaa tccttcgtaa acgatagtcg ccatccccgc
    53941 cagcagcggc gcgtagacga tatacgaatg ccccaccacc cagccaatat ccgaagcaca
    54001 aaagaacaca ccgcccgctt tgccgccaaa aatggtttcc atcgaggtcg ccagcgccac
    54061 cgcatatccg ccaacgtcac gctgcacgcc tttaggtttg ccggtcgtgc cggaggtgta
    54121 gagaatgcag gaggtttcgt tggattccag ccacgccacc ggtacccgcg cgccgatgtg
    54181 ttgatggcgc aacgacgcga aatcgacatc ccgcccgctg acgcgtgcca ttttcgccag
    54241 cccgcgatcc accagcaaaa catggcgtgg ctggtgctgc gcctgactta tcgcatcgtc
    54301 gagtaatttt ttatagggaa tgattttgcc accgcgcgct ccggcatcag ccgagacaat
    54361 cagcaccggt ttagcatcat caattcgcgc cgccacgctg tgcgaggcaa atccaccaaa
    54421 caccaccgag tgaatagcgc caatgcgcgc gcaggccagc agagtaatat gcgcttcggc
    54481 aatcatcggc atatacacca gcacccgatc gccacgctgc acgcccaatg aacgcaacat
    54541 tgaggccacc gcgttcactt cgtcatgcag ctgacgaaag gtaaaggtgc gctcttcttc
    54601 tgtttccgaa gagacggcaa tcagcgccag cgcctctggc tgtttctcca gccagcggtc
    54661 gatagcgttg tgacacaagt tggttcggcc ttcacaaaac caacgggcaa acggcggatt
    54721 gctgtgatcg agcgtttgcg taaagggcgt ctgccagtca atacgccggg cctgctcggc
    54781 ccagaactgc tccggttcgt taatcgaacg ctgataaaat tcgctaaaag acataatgct
    54841 ctcctgttga acttacgcct taccgctgtt tgcttaaatg acgtacaggt cgagatactc
    54901 attgaccggc atctgttcca ggcgagctct gtcgagagaa acgtccagaa tgcgctgctg
    54961 ttggcgagtc gggaactggc gcgcgagatt gattttgaat ttatcgacca gcttcggaat
    55021 gccatcctga cggcggcgag catgaccaat tgggtactcc accaccactt cttcaaatcg
    55081 cgtgccgtcg gtgaactcaa gggttatggc attggcgatg gcgcgttttt ccgggtcgtg
    55141 gtagtcagca gtaaatgccg gatcttcaaa gcaattgatc ttctcgcgca gggcgtcaat
    55201 gcgtttatct tgcgcaacgt tgtcctcgta atctgccgcc gttaagcgcc cgaatagcag
    55261 cgggatcgcc accatgtact gaatgcagtg atcgcggtct gccgggttat tgagcggccc
    55321 ttttttgtcg atgatgcgaa tacaggcttc gtgggtgcga atggtcactt tttcgatatc
    55381 tgccgccgtt ttgcctgctg cctgcatctg ttcatagagc gtcatcgctg cttcaactgc
    55441 cgtctgggag tggaactccg ccgggaagga gattttgaac agcacatttt ccatgacgta
    55501 agaaccgtac gggcgctgga agcggaacga ttcacctttg aaggagacgt cgtagaagcc
    55561 ccacacaggc gcagtcaggg ctgacgggta acccatttcg cccgttttcg ccatcagtgc
    55621 cagacgtacc gcgcgggaag tggcatcgcc cgccgcccag gatttacgcg tgccggtgtt
    55681 cggcgcatgg cgataggtgc gcagcgactg accgtccacc cacgccagcg aaacagcgtt
    55741 gaggatttcc tcgcgggtca ggccgagcat ttcggcgacc acggcggtgg aagccacttt
    55801 cactaacaga acgtggtcga ggccgacgcg gttaaaggag ttttccagcg cgatgcagcc
    55861 ctgaatttca tgggctttga tcattgcggt cagcacctgt ttcatggtca acggcgcttt
    55921 gccgctggcg accgcgttgc gcgaaagcca gtccgccgtt gccagaatgc cgccgaggtt
    55981 gtcggaagga tggccccact ccgccgccag ccaggtatcg ttgaaatcaa gccagcggat
    56041 catcgcgcca atgttaaatg ccgcctggac ggggtcgagc tggaactgag ttccggggac
    56101 gcgcacgccg ttagggacga cagtgccagg aacaattggc cccagcagtt ttttacaggc
    56161 cgggtattcg agagcttcca gaccgcagcc gagcgtgtcg agcaggcagt aatgtgcggt
    56221 gtcgtaggcc actttagagc taatttcgta gttcatcacg taatcgacga tatcaacgat
    56281 ttcacgatca aattccgggc ggatgttgtt gatttgagct gacattgggt acgtttcctt
    56341 attgttattc gtagaggttt actggcgctt atccagcgcg acaaactggc ggtcttccgg
    56401 tccaacataa ttggcggaag gacggataat tttgttgtcc tgacgttgtt cgataatgtg
    56461 cgccgcccag ccagtgacac gggcgataac aaacagtggt gtgaacatct cggtgggaac
    56521 gcccatcatg ttgtaggaaa cagcggagaa ccagtcgaga ttggggaaca tctttttgct
    56581 ctcccacatc accgtttcca ggcgatcggc gatgttgtac atcttcagcg agccgccttc
    56641 ctgcgagagc tgcttcgcca cacgtttgat cacctgatga cgcgggtcgg cgatggtata
    56701 aaccggatga ccaaaaccaa tgaccacttc tttgttttcc acccgcttgc ggatatcggc
    56761 ttcggcttcg tccggcgttt cgtagcgttg ctggatctcc agcgacactt cattcgcccc
    56821 gccgtgcttc ggcccgcgca gtgcaccaat cgcgccaata atcgcggaat acatatcaga
    56881 gccggtgccc gcaatcaccc ggctggtaaa ggtggaggcg ttaaactcgt gttcggcgta
    56941 cagcaccagc gaaatatgca tcgccttttc ccagctttgc gatggctttt cgccgtgcag
    57001 cagatgcagg aagtgaccac cgatggagtc gtcatcggtt tccggctgga tgcgttcgcc
    57061 gttgtggctg tagtgatacc agtagagaag aatcgagcta agtgacgcca gcagtttgtc
    57121 ggcaatatcc cgcgcgccag agacggtatg cccctctttt tctggcagcg tgcagccgag
    57181 cgcggaaacg ccggtgcgca taacatccat cgggtgcgac gccgccggta aggcttccag
    57241 cacggtacgc acattagctg gtaaaccacg cagggctttc agtttcgttt tgtaagcggc
    57301 gagttcgtca cgggtcggca gtttgccgtg gatcagcaga tgcgccactt cttcaaattc
    57361 gcaatgttcc gccagatcaa gaatgtcgta gccgcggtaa tgcaaatcat tgccgctttt
    57421 acccacggtg cagagcgccg tattgcccgc cggaacgcca gaaagtgcta ccgatttttt
    57481 cggtttaatg acatgggtac tgttttgcag gatcgttgtg tcgctcatga tgtcctcgtc
    57541 atttgtaggg tcgagatatt tttcgttgtg ttttatttca cctggctacg ggcaaacagc
    57601 tcgtcgagct tctcttcgta ctggtagtag ttgatgcttt cgtacagctc gttgcgggtc
    57661 tgcatggtgt cgatgacgct tttctgcgtc ccttcctgac gcaggacgtt gtagacatgc
    57721 tcagcggcgc ggttcatggc gcggaacgct gaaagtgggt acagcgccat tgcgacatgg
    57781 gcgctgcgta attcgtcggt ggtaaacagc ggtgttgcgc caaattcggt gatgttggcg
    57841 aggatcggca cctgcaccgc atcggcaaac tggcggtaca tggcgagttc ggtaatcgcc
    57901 tccgggaaca acatctcggc acccgcttca acataggcct gcgcacgctc aatcgccgca
    57961 tccagcccct ctaccgccag cgcatcggtg cgcgccatga tcacaaaatc aggatcggtt
    58021 ttcgcatcca ccgccgcgcg gatccgatcc accatctcct ctttcgagac gatcgctttg
    58081 ttcggacgat gaccgcagcg tttcgcacca acctgatctt caatatgcag tcctgccgca
    58141 ccggctttaa tcatcgattt cacggtgcgc gccacgttaa aagcagaaga accaaaaccg
    58201 atatccgcat ccaccagcag cggcagcgaa caaacgtcgg tgatacgacg aatgtcggtc
    58261 agcacatcat caagggtaga aataccgaga tcgggcagcc ccagcgaccc tgccgccacg
    58321 ccaccgccgg aaagataaat tgcctgatat ccggcacgct gcgccaacag cgcatggtta
    58381 gcgttgatgg tgccaacaat ctgcaatgga ttttctttag acagtgcagc gcgaaacgct
    58441 ttacctggag agtgtagaga catagcccat cctttgttat caacttgtta tttgcgttga
    58501 taaagacaaa gcaaggggtg tgccacaatt gatcgaataa aattaaatgt ttaatttcaa
    58561 tttgttataa ataaagtcat gaagtaatcc gcaaatatgc gtttcagtta acgtttcagt
    58621 caatgtttca ccgcgtttca ttgcaacaat tatgaaacaa gactaaaccc aatattcggt
    58681 ttcttaactt tgcggtgcgc tatggcacat ccaccacggc tgaatgacga caaaccggtt
    58741 atctggacgg tatctgtaac gcgcctgttc gagctgtttc gcgatatcag cctcgagttt
    58801 gatcatctgg cgaacattac ccctatccag cttggctttg aaaaagcggt gacctacatc
    58861 cgcaaaaaac tggcaaacga acgctgtgac gccatcatcg ctgctggctc taacggcgcg
    58921 tacctgaaaa gccgcctgtc agtgccagtt attttgatta aaccgagcgg ctacgatgtg
    58981 ttacaggcac tggcaaaagc cggaaaactc acctcttcta tcggcgttgt cacttatcag
    59041 gaaaccattc cagcgctggt ggcgtttcaa aaaaccttta atttgcgcct cgaccaacgt
    59101 agctacatta ccgaggaaga cgcacgcggg cagattaacg agctaaaagc taacggcacc
    59161 gaagcggtgg tcggcgcggg gctgattacc gatctggcag aagaagccgg aatgaccgga
    59221 atttttatct attccgccgc caccgtgcgc caggcgttca gcgatgcgct ggatatgacg
    59281 cgcatgtcgt tacgccataa cactcacgat gccacccgca acgccctgcg tactcgttac
    59341 gtgctgggcg atatgctcgg tcaatcacca cagatggaac aggtacggca gactattttg
    59401 ctgtatgccc gctccagtgc agcggtgttg attgaagggg aaacggggac gggcaaagag
    59461 ctggcggccc aggcgattca tcgggaatat tttgcccgcc acgatgcgcg acagggcaaa
    59521 aagtcgcatc cgtttgttgc cgtcaactgc ggggcgattg ccgaatcgtt gctggaagca
    59581 gagctgtttg gctatgagga aggggcgttc accggctcgc gacgcggagg tcgcgccggg
    59641 ctgttcgaaa ttgcccacgg cggtacgctg tttctggatg agattggcga aatgccgcta
    59701 cctttgcaga ctcgcctgtt acgggtgctg gaagaaaaag aggtcacccg cgtcggcggg
    59761 catcagcctg ttccggtgga tgtgcgagtc attagcgcca ctcactgcaa tctggaagaa
    59821 gatatgcggc aagggcagtt tcgccgtgac ctgttttatc ggctgagcat tttacgcctg
    59881 caactgccac cactgcgtga gcgggtggca gatattctgc cgctggcgga aagctttttg
    59941 aaagtgtctc tggctgcact tgctgccccg ttttcttccg cattacgcca gggattacag
    60001 gcaagcgaaa ccgtgctggt gcactacgac tggccgggca atattcgaga actgcgcaat
    60061 atgatggagc gactggcgct gtttttaagt gtggaaccga cgccggattt aacgccgcag
    60121 tttttgcagc tgctactgcc ggagctggcg cgcgagtcgg cgaaaattcc cgctccacgc
    60181 ttactgacac cacaacaggc actggagaaa tttaaaggcg ataaaacagc agcggcgaat
    60241 tatttaggca taagccggac gacgttctgg cggcggctga aaaactgaac tgccgaatgc
    60301 agcgcgaacg ctttatccgg cataaatttc aatagatgat gatcaccgat aaacttacgc
    60361 atcggggatg tttacatagg tttcagaatt acttcttctt ataaatattt gccgtgccgt
    60421 gaatcttatt gtcagtttga ccagaggtca gcaccaacac atcagcccct ttttcatcgg
    60481 cttttttgat taaatcttct tttgcatctg cagtcgacat ttcattactg gttgaaatat
    60541 cacctatttt ttcatattgc gattcaactt tttcaaattc cgctttggtc atcaattcag
    60601 cggcatagac attggtaacg gcaaacgcta acgcaccgac taacatttta gagattattt
    60661 tcatattacg gctccagagt tgttatcaca ctatcaccca ccgacattat tgggttgatt
    60721 aataattagt tcagtcaggg cataaacgcc agtaaccgga aataagacac tttgtgatct
    60781 ctctcgcacc cgattatttt tggtagaaat atgatgcagt tagttcactt atttatggat
    60841 gaaatcacta tggatccttt gcatgccgtt tacctgaccg taggactgtt cgtgattact
    60901 ttttttaatc cgggagccaa tctctttgtg gtagtacaaa ccagcctggc ttccggtcga
    60961 cgcgcagggg tgctgaccgg gctgggcgtg gcgctgggcg atgcatttta ttccgggttg
    61021 ggtttgtttg gtcttgcaac gctaattacg cagtgtgagg agattttttc gcttatcaga
    61081 atcgtcggcg gcgcttatct cttatggttt gcgtggtgca gcatgcgccg ccagtcaaca
    61141 ccgcaaatga gcacactaca acaaccgatt aacgccccct ggtatgtctt ttttcgccgc
    61201 ggattaatta ccgatctctc taacccgcaa accgttttat tttttatcag tattttctca
    61261 gtaacattaa atgccgaaac accaacatgg gcacgtttaa tggcctgggc ggggattgtg
    61321 ctcgcatcag ttatctggcg cgtttttctt agtcaggcgt tttctttgcc cgcagtgcgt
    61381 cgtgcttatg ggcgtatgca acgcgttgcc agtcgggtta ttggtgcaat tattggtgta
    61441 ttcgcgctac gcctgattta cgaaggggtg acgcagcggt gagcagataa tttactctct
    61501 acgtaatcaa cctgatttgc cgaatcccga ccatcggcga tgatagcccc cgtaatgcgt
    61561 ggtccttatt tccgataagt ttacttgagt ctttagcttc ccatttcagc ccactctccc
    61621 ctgtgtgggc ttttttcatt gctctgaccc gtcctgaata gccaaacaat ggtaatgttc
    61681 cactttgcag ggatgataat aaggggagct gattcagaag agttatgaaa agttggaccg
    61741 ccatcgaggc ctcgaacccc gtaccataca acaatcctgg ttgtcgtctg ctcttcctga
    61801 tgagctaatg gcggtttttg atatgagcct tacagcatat tttctgtcag gcttgagtgc
    61861 gatgcgtaag atacagaaaa gaccccacac aaacatgatg gaattatgat ttttttaata
    61921 tgaatgtcaa cccacacatt cacaaaacat ctgcatgaga tcatttttta tacaccgtat
    61981 agctgcgaaa ccactgctga tgctttttta agcgaagtgt cgccgatcac aacaaaatat
    62041 cggcgaaaaa ggcggtggaa tgccatttgg catacggata tacagatgaa atgatcacct
    62101 gctttgcgat tcactctctg gtggaataac aactcgcagg tggaaccctt tctgaactaa
    62161 agagtgtagc ggattttaac gtgcttattt acaggataat atctgtcacc taattcctgc
    62221 actctattag atatccattg atttaatcgc gcatcatctc tgtatgtttt tacgtccaga
    62281 acagagagca ataacatcac cagtgattca ggaaaaacag ggtagatatt ttttaagtgt
    62341 aataataatc catgcgcaag cgcctgctta atgggtgcgt cggtctccgg aatagacgac
    62401 atttcgcgaa gccatttttc cacatgcata cactctttcc ctttagggaa aagtctcagc
    62461 aaaggcatat ggtgttcaag aagaatacat ttgcaatctg ttcgcactga atcgataaac
    62521 tgcactaatt tcttaagccc ggtaaagcgt ccagcctgca cgtttgcttt aatatcaaga
    62581 aacgcatcgt cctggtcata tacccaaact gtcccttttt gattttcaag tatgcgaaaa
    62641 acaaacttaa ccgggtgatc agggtaatcc atccagtcat aaaatccagg gaaatcatta
    62701 aactgcacat ttatggtgag tgcgttatcg tctgaccact cacagtctcg tacggcagcc
    62761 atgagatgaa tgagtttgtc gtcatcgact aaatcatcac gacaatcata gggataatca
    62821 taatattgtt taatcaaaac cttgaatata tcgacacagg agaccaggga gcttttgata
    62881 tcctctggta actgattgtt ttttactgta ttaagtatgc tttgttgcgt atatggcatc
    62941 aaat
//
LOCUS       DAPOZO010000017        62522 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPOZO010000017 DAPOZO010000000
VERSION     DAPOZO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.017"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            56..415
                     /gene="gutM"
                     /locus_tag="ROI21_001137"
     CDS             56..415
                     /gene="gutM"
                     /locus_tag="ROI21_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDX5110088.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVVIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMYAGDLQPDVIFPN
                     DPLSQNALSLALKLKRG"
     gene            482..1255
                     /gene="srlR"
                     /locus_tag="ROI21_001138"
                     /gene_synonym="gutR"
     CDS             482..1255
                     /gene="srlR"
                     /locus_tag="ROI21_001138"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDX5110089.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEKKGIDVIITGESNE"
     gene            1248..2213
                     /gene="gutQ"
                     /locus_tag="ROI21_001139"
     CDS             1248..2213
                     /gene="gutQ"
                     /locus_tag="ROI21_001139"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDX5110090.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(2210..3724)
                     /gene="norR"
                     /locus_tag="ROI21_001140"
     CDS             complement(2210..3724)
                     /gene="norR"
                     /locus_tag="ROI21_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDX5110091.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            3911..5350
                     /gene="norV"
                     /locus_tag="ROI21_001141"
     CDS             3911..5350
                     /gene="norV"
                     /locus_tag="ROI21_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDX5110092.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEEK"
     gene            5347..6480
                     /gene="norW"
                     /locus_tag="ROI21_001142"
     CDS             5347..6480
                     /gene="norW"
                     /locus_tag="ROI21_001142"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDX5110093.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDTVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(6558..8798)
                     /gene="hypF"
                     /locus_tag="ROI21_001143"
     CDS             complement(6558..8798)
                     /gene="hypF"
                     /locus_tag="ROI21_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDX5110094.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWISHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWAREMNLPTQTMLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRTRLSHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RILHTA"
     gene            complement(8951..9478)
                     /gene="hydN"
                     /locus_tag="ROI21_001144"
     CDS             complement(8951..9478)
                     /gene="hydN"
                     /locus_tag="ROI21_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDX5110095.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(9627..10637)
                     /gene="ascG"
                     /locus_tag="ROI21_001145"
     CDS             complement(9627..10637)
                     /gene="ascG"
                     /locus_tag="ROI21_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDX5110096.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIAL
                     SQ"
     gene            10897..12354
                     /gene="ascF"
                     /locus_tag="ROI21_001146"
     CDS             10897..12354
                     /gene="ascF"
                     /locus_tag="ROI21_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDX5110097.1"
                     /translation="MAKNYAALARSVIAALGGVNNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFEPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            12363..13787
                     /gene="ascB"
                     /locus_tag="ROI21_001147"
     CDS             12363..13787
                     /gene="ascB"
                     /locus_tag="ROI21_001147"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDX5110098.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRAVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(13866..14285)
                     /locus_tag="ROI21_001148"
     CDS             complement(13866..14285)
                     /locus_tag="ROI21_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5110099.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(14282..14593)
                     /locus_tag="ROI21_001149"
     CDS             complement(14282..14593)
                     /locus_tag="ROI21_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HDX5110100.1"
                     /translation="MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(14760..15230)
                     /gene="hycI"
                     /locus_tag="ROI21_001150"
     CDS             complement(14760..15230)
                     /gene="hycI"
                     /locus_tag="ROI21_001150"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDX5110101.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(15223..15633)
                     /gene="hycH"
                     /locus_tag="ROI21_001151"
     CDS             complement(15223..15633)
                     /gene="hycH"
                     /locus_tag="ROI21_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDX5110102.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(15630..16397)
                     /gene="hycG"
                     /locus_tag="ROI21_001152"
     CDS             complement(15630..16397)
                     /gene="hycG"
                     /locus_tag="ROI21_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDX5110103.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(16397..16939)
                     /gene="hycF"
                     /locus_tag="ROI21_001153"
     CDS             complement(16397..16939)
                     /gene="hycF"
                     /locus_tag="ROI21_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDX5110104.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(16949..18658)
                     /gene="hycE"
                     /locus_tag="ROI21_001154"
     CDS             complement(16949..18658)
                     /gene="hycE"
                     /locus_tag="ROI21_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDX5110105.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLASHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(18676..19599)
                     /gene="hycD"
                     /locus_tag="ROI21_001155"
     CDS             complement(18676..19599)
                     /gene="hycD"
                     /locus_tag="ROI21_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDX5110106.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(19602..21428)
                     /gene="hycC"
                     /locus_tag="ROI21_001156"
     CDS             complement(19602..21428)
                     /gene="hycC"
                     /locus_tag="ROI21_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDX5110107.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTNNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(21425..22036)
                     /gene="hycB"
                     /locus_tag="ROI21_001157"
     CDS             complement(21425..22036)
                     /gene="hycB"
                     /locus_tag="ROI21_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDX5110108.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(22161..22622)
                     /gene="hycA"
                     /locus_tag="ROI21_001158"
     CDS             complement(22161..22622)
                     /gene="hycA"
                     /locus_tag="ROI21_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDX5110109.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            22834..23184
                     /gene="hypA"
                     /locus_tag="ROI21_001159"
     CDS             22834..23184
                     /gene="hypA"
                     /locus_tag="ROI21_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDX5110110.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            23188..24060
                     /gene="hypB"
                     /locus_tag="ROI21_001160"
     CDS             23188..24060
                     /gene="hypB"
                     /locus_tag="ROI21_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDX5110111.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     TPEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            24051..24323
                     /gene="hypC"
                     /locus_tag="ROI21_001161"
     CDS             24051..24323
                     /gene="hypC"
                     /locus_tag="ROI21_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDX5110112.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            24323..25444
                     /gene="hypD"
                     /locus_tag="ROI21_001162"
     CDS             24323..25444
                     /gene="hypD"
                     /locus_tag="ROI21_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDX5110113.1"
                     /translation="MRFVDEYRSPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEEPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            25441..26451
                     /gene="hypE"
                     /locus_tag="ROI21_001163"
     CDS             25441..26451
                     /gene="hypE"
                     /locus_tag="ROI21_001163"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDX5110114.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVTELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            26525..28603
                     /gene="flhA"
                     /locus_tag="ROI21_001164"
     CDS             26525..28603
                     /gene="flhA"
                     /locus_tag="ROI21_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDX5110115.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGNTMLGVLKLAQCEEKVFTTTNLSLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            28648..29076
                     /locus_tag="ROI21_001165"
     CDS             28648..29076
                     /locus_tag="ROI21_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HDX5110116.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEGCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(29115..29459)
                     /locus_tag="ROI21_001166"
     CDS             complement(29115..29459)
                     /locus_tag="ROI21_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDX5110117.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYDALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(29536..29670)
                     /locus_tag="ROI21_001167"
     CDS             complement(29536..29670)
                     /locus_tag="ROI21_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110118.1"
                     /translation="MIPELIISGEEANGEFLMVIPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            29746..32307
                     /gene="mutS"
                     /locus_tag="ROI21_001168"
     CDS             29746..32307
                     /gene="mutS"
                     /locus_tag="ROI21_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDX5110119.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAENFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIAPGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            32413..33069
                     /gene="pphB"
                     /locus_tag="ROI21_001169"
     CDS             32413..33069
                     /gene="pphB"
                     /locus_tag="ROI21_001169"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDX5110120.1"
                     /translation="MPSTRYQKINAHHYRYIWAVGDIHGDYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FELNETEQQEAINLLLKFHRLPHIIEITNDIIKYVIAHADYPGNEYLFGKEIAERELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(33120..33887)
                     /locus_tag="ROI21_001170"
     CDS             complement(33120..33887)
                     /locus_tag="ROI21_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDX5110121.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            34083..34991
                     /locus_tag="ROI21_001171"
     CDS             34083..34991
                     /locus_tag="ROI21_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDX5110122.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSNIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            34988..36250
                     /locus_tag="ROI21_001172"
     CDS             34988..36250
                     /locus_tag="ROI21_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDX5110123.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDTPL
                     VTGGSGLAIGLARQWAQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSIETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAHKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            36247..36885
                     /locus_tag="ROI21_001173"
     CDS             36247..36885
                     /locus_tag="ROI21_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDX5110124.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            36890..37666
                     /locus_tag="ROI21_001174"
     CDS             36890..37666
                     /locus_tag="ROI21_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDX5110125.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            37755..39119
                     /locus_tag="ROI21_001175"
     CDS             37755..39119
                     /locus_tag="ROI21_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDX5110126.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKCQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(39158..39394)
                     /locus_tag="ROI21_001176"
     CDS             complement(39158..39394)
                     /locus_tag="ROI21_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110127.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(39405..40832)
                     /locus_tag="ROI21_001177"
     CDS             complement(39405..40832)
                     /locus_tag="ROI21_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDX5110128.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(40832..41425)
                     /locus_tag="ROI21_001178"
     CDS             complement(40832..41425)
                     /locus_tag="ROI21_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDX5110129.1"
                     /translation="MRLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRVGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQVRNFSQENE"
     gene            41572..41979
                     /locus_tag="ROI21_001179"
     CDS             41572..41979
                     /locus_tag="ROI21_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDX5110130.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEKELFMQLVRKMMSK"
     gene            complement(42100..43092)
                     /gene="rpoS"
                     /locus_tag="ROI21_001180"
     CDS             complement(42100..43092)
                     /gene="rpoS"
                     /locus_tag="ROI21_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDX5110131.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(43155..44294)
                     /gene="nlpD"
                     /locus_tag="ROI21_001181"
     CDS             complement(43155..44294)
                     /gene="nlpD"
                     /locus_tag="ROI21_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDX5110132.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(44434..45060)
                     /gene="pcm"
                     /locus_tag="ROI21_001182"
     CDS             complement(44434..45060)
                     /gene="pcm"
                     /locus_tag="ROI21_001182"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDX5110133.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(45054..45815)
                     /gene="surE"
                     /locus_tag="ROI21_001183"
     CDS             complement(45054..45815)
                     /gene="surE"
                     /locus_tag="ROI21_001183"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDX5110134.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(45796..46845)
                     /gene="truD"
                     /locus_tag="ROI21_001184"
     CDS             complement(45796..46845)
                     /gene="truD"
                     /locus_tag="ROI21_001184"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDX5110135.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(46842..47321)
                     /gene="ispF"
                     /locus_tag="ROI21_001185"
     CDS             complement(46842..47321)
                     /gene="ispF"
                     /locus_tag="ROI21_001185"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDX5110136.1"
                     /translation="MRIGHGFDVHAFGGEGPLIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(47321..48031)
                     /gene="ispD"
                     /locus_tag="ROI21_001186"
     CDS             complement(47321..48031)
                     /gene="ispD"
                     /locus_tag="ROI21_001186"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDX5110137.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(48050..48361)
                     /gene="ftsB"
                     /locus_tag="ROI21_001187"
     CDS             complement(48050..48361)
                     /gene="ftsB"
                     /locus_tag="ROI21_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDX5110138.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(48555..48878)
                     /locus_tag="ROI21_001188"
     CDS             complement(48555..48878)
                     /locus_tag="ROI21_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDX5110139.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(48928..49533)
                     /gene="cysC"
                     /locus_tag="ROI21_001189"
     CDS             complement(48928..49533)
                     /gene="cysC"
                     /locus_tag="ROI21_001189"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDX5110140.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(49533..50960)
                     /gene="cysN"
                     /locus_tag="ROI21_001190"
     CDS             complement(49533..50960)
                     /gene="cysN"
                     /locus_tag="ROI21_001190"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDX5110141.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(50962..51870)
                     /gene="cysD"
                     /locus_tag="ROI21_001191"
     CDS             complement(50962..51870)
                     /gene="cysD"
                     /locus_tag="ROI21_001191"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDX5110142.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            52122..53159
                     /gene="iap"
                     /locus_tag="ROI21_001192"
     CDS             52122..53159
                     /gene="iap"
                     /locus_tag="ROI21_001192"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDX5110143.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSAKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   53241..53818
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=53305..53330
                     /rpt_unit_seq="tttatccccgctggcgcggggaacac"
     gene            complement(53915..54208)
                     /gene="cas2"
                     /locus_tag="ROI21_001193"
     CDS             complement(53915..54208)
                     /gene="cas2"
                     /locus_tag="ROI21_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDX5110144.1"
                     /translation="MSMVVVVTENVPPRLRGRIAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGVRLVSFLPVDNQ"
     gene            complement(54205..55128)
                     /gene="cas1e"
                     /locus_tag="ROI21_001194"
     CDS             complement(54205..55128)
                     /gene="cas1e"
                     /locus_tag="ROI21_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HDX5110145.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSSKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(55125..55775)
                     /gene="cas6e"
                     /locus_tag="ROI21_001195"
     CDS             complement(55125..55775)
                     /gene="cas6e"
                     /locus_tag="ROI21_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDX5110146.1"
                     /translation="MYLSRITLHTGQLLPAQLLHLVGRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(55757..56503)
                     /gene="cas5e"
                     /locus_tag="ROI21_001196"
     CDS             complement(55757..56503)
                     /gene="cas5e"
                     /locus_tag="ROI21_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDX5110147.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDASYRLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(56514..57569)
                     /gene="cas7e"
                     /locus_tag="ROI21_001197"
     CDS             complement(56514..57569)
                     /gene="cas7e"
                     /locus_tag="ROI21_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HDX5110148.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECVSFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(57584..58120)
                     /gene="casB"
                     /locus_tag="ROI21_001198"
     CDS             complement(57584..58120)
                     /gene="casB"
                     /locus_tag="ROI21_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HDX5110149.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAVKTPDDLLRQLRRAVKLLNGSVNLISLAEDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(58117..59679)
                     /gene="casA"
                     /locus_tag="ROI21_001199"
     CDS             complement(58117..59679)
                     /gene="casA"
                     /locus_tag="ROI21_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HDX5110150.1"
                     /translation="MNSFSLLTTPWLPVRYKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLESEKLREALLSLEHAFQFGPDSPSFM
                     QDFEALTGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLDKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRHIE
                     EGQDADELLSKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQNA
                     KAAREKKQEAAE"
     gene            complement(59777..62476)
                     /locus_tag="ROI21_001200"
     CDS             complement(59777..62476)
                     /locus_tag="ROI21_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDX5110151.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKSNCFGLTDYFRQLGFADTEQAAQFFAWLLCWHDTGKFARSFQQLYLHP
                     QLKVPEGARKNYEKISHSTLGYWLWHHYLSEYEELLPSSSLSPRKLKRVMEMWMPITT
                     GHHGRPPDRIDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFTGI
                     NTLFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQCDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVHSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLESSVDCDMDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIASVVKPYANGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRQWCIEQRKDPEMANVILVTDDESCGYSAMEGLTGKVG"
BASE COUNT        14802 a        17033 c        16193 g        14494 t
ORIGIN      
        1 atgcgctacg ctcatcaggc atacaggact tccgccacta cattacggag aagttatggt
       61 atccgcactc atcaccgtcg ccgttatcgc ctggtgtgcg caactggcct taggcggttg
      121 gcaaatttct cgttttaacc gtgccttcga cacactatgc cagcaagggc gggttggcgt
      181 gggccgttcc agcgggcgct ttaaaccgcg ggtcgtggtc gtcatcgccc tggacgatca
      241 gcaacgcatc gtcgacacct tgtttatgaa aggactgacc gtcttcgccc gaccacaaaa
      301 aattcccgca attaccggta tgtatgcggg tgatttacag cccgatgtga tctttcccaa
      361 tgatccacta tcacagaatg ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg
      421 ttgtgaatgt tacttgcttg cgaagttatc attttgaaac ctaaatcagg taatcacgcc
      481 catgaaacct cgtcagcgtc aggccgccat tctggagtat ctgcaaaagc agggtaaatg
      541 ctcggttgaa gaattggcgc aatactttga caccacaggc acaaccattc gcaaagatct
      601 ggtcattctg gaacatgccg gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa
      661 agaggaatcc gatccgccta tcgatcataa aacactcatc aacacccaca agaaagagct
      721 gattgcagaa gctgccgtta gttttatcca tgatggcgat tcgatcattc ttgatgctgg
      781 cagtaccgtt ttgcagatgg ttcccctgct ctcgcgcttt aataacatca cagtgatgac
      841 caacagcctg catatcgtca atgcgctatc cgaactggat aacgaacaaa ctatcctgat
      901 gccaggcgga acatttcgta aaaaatcggc ctcatttcac gggcaactgg cagagaatgc
      961 cttcgagcat ttcaccttcg ataaattgtt tatgggcacc gacggcatcg atctcaatgc
     1021 gggcgtaacc acgttcaacg aggtttatac cgtcagtaag gcaatgtgca atgccgcgcg
     1081 cgaagtgatt ttgatggcag actcatcaaa gtttggccgt aaaagcccca acgtagtttg
     1141 cagtcttgaa agcgtcgata agctaattac cgacgcaggt atcgatccgg cgtttcgtca
     1201 ggcgctggaa aagaaaggga tcgatgtgat cataaccgga gagagcaatg agtgaagcac
     1261 tactgaacgc gggacgtcag actttaatgc tggagttgca ggaagcaagc cgtttaccgg
     1321 aacgtctggg cgatgatttt gttcgcgccg ccaatatcat cctgcactgc gaaggcaaag
     1381 tggtggtttc gggaattggc aaatcgggcc acattggtaa gaaaatcgcc gcaacgcttg
     1441 ccagtaccgg cacaccggct ttttttgtcc atccggcaga ggcactgcac ggcgatctgg
     1501 ggatgatcga aagccgcgat gtgatgctgt ttatctctta ctccggtggc gcgaaggaac
     1561 tggatctgat tattccgcgt ctggaagata aatctatcgc actgctggcg atgaccggca
     1621 aaccgacgtc accgctgggc ctggcggcga aagcggtgct ggatatctcc gtagaacgcg
     1681 aagcctgtcc gatgcacctt gcgccgacct ccagcaccgt gaataccctg atgatgggtg
     1741 acgcgctggc gatggcggtc atgcaggcgc gcggatttaa tgaagaagat tttgcccgct
     1801 cccaccctgc tggagcattg ggtgctcgct tgctgaataa agtgcatcat ctgatgcgcc
     1861 gtgatgacgc tatcccgcag gtagcgttaa ccgccagcgt gatggatgcg atgctggaac
     1921 tcagccgcac tggtctgggg ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg
     1981 tctttaccga cggcgattta cgtcgctggc tggttggcgg cggcgcactc accacgccag
     2041 ttaatgaagc gatgacgaca ggcggcacca cattacaggc gcaaagccgt gccatcgacg
     2101 ccaaagagat cctgatgaag cgcaaaatca ctgccgcacc ggtggtggat gaaaacggca
     2161 aactcaccgg cgcaattaac ttgcaggatt tctatcaggc cgggattatt taatccttca
     2221 atcccagacg tttcgccagc cgatgcaggt tggcgacgtc ggtttccagc atccgcgcac
     2281 aggcagccca gttgtgatga ttttgtgcca gtgcctgacg aatagtttca cgctggaacg
     2341 cttctgtcgc ttcacgcagg ttttgcttaa caacgggcac cgccgccgct tctggcggcg
     2401 gcaacgtcac ctcaggaaaa gcaaaatgtt gcgcctcaag aatcacttca tcgccgctgc
     2461 gagtggctct cgacagtact accgcccgat gaatagcatg ttccagttcg cgcacgtttc
     2521 ccggaaaact gtagtgttgc agtaaatttc gcgccccggc acttaatacc acgcgggaga
     2581 gccccagccg caaacgacac tgctcgcaga aataccccgc cagcagaatg acatcatcgc
     2641 cccgctcacg cagcggcggc accgaaagtg gaaacacgct caggcgatga aacaaatcgg
     2701 cgcggaatcg ccctgccagc acctcttcgc gtaaatcgcg gttagtcgcc gccagcacgc
     2761 gcacatcgac ccgcaaactg cggtcatcgc caacgcgctg aatatcgcca tactgcaaca
     2821 ccctcagcag cttggcctgc aatgccaacg acaactcgcc gatctcatcg agaaacagtg
     2881 tgccgttatc cgccatttca aacttcccgc tgcggttact gatagcacca gtaaacgccc
     2941 ctttcacatg cccgaacagt tcactttccg ccacactttc cggcagtgcg gcacagttga
     3001 gatagaccag cggattcacc gcccttggcg aggcttcatg aatcgctttc gccaccagct
     3061 ccttaccggt tcccgtctca ccgctgatta agacgttgag atcggacgcc gccacaatct
     3121 caatctcttt ttttaattgc gtcatacctg gcgacagacc gatcatctgc gtctgtttca
     3181 ccgcttcaaa cggcgtggca tcgcctggca gcatattctg gctttccagt tgttcaatca
     3241 gcaacgcatt gcttaacgct cccgccgcca gcgcggcaat caggcgtaac tcttcgtcgc
     3301 tgaaaacatc gaactgatcg ggctgcatcc cgtcgagcgt caatgcgccg atcaggtttt
     3361 gcccggcaaa caatggcaga ccaacgcagg cgtgaacctt cagactctcc tgcccgggaa
     3421 tcaaaccgtc atagggatcg ggcaattcgc tgtctgcggg aaagcgcacc acgtccccgg
     3481 cgcgggcaat cgcttccagc cgtggatgcc cttccagcgc aaagcgtcta ccgagtacat
     3541 cctttgccag cccgtcgatg gcaagcggaa taaactgccg cgagtcgtaa cgtagcaatg
     3601 cagacgcatc gcactccagc acctgacgca gcgtggtgat caggcgctga aaacgatcct
     3661 gatgaccaat cccacgctgc aattcgatgg cgatattcgc cagcacatca acggaaaaac
     3721 tcatctttgc ctcactgtca atttgactat agatattgtc atatcgacca tttgattaat
     3781 agtcattttg actactcatt aatgagcata attttattta tagagtaaaa acaatcagat
     3841 aaaaaactgg cacgcaatct gcaattagca agacatcttt ttagaacacg ctgaataaat
     3901 tgaggttgct atgtctattg tggtgaaaaa taacattcat tgggttggtc aacgtgactg
     3961 ggaagtgcgt gattttcacg gcacggaata taaaacgctg cgcggcagca gctacaacag
     4021 ctacctcatc cgcgaagaaa aaaacgtgct gatcgacacc gtcgaccata aattcagccg
     4081 cgaatttgtg cagaacctgc gtaatgaaat cgatctggcg gatatcgatt acatcgtgat
     4141 taaccatgct gaagaggacc acgccggggc gctgaccgaa ctgatggcgc aaattcccga
     4201 tacgccgatc tactgtactg ctaacgctat cgactcgata aatggtcatc accatcatcc
     4261 ggagtggaat tttaatgtgg tgaaaactgg cgacacgctg gatatcggca acggcaaaca
     4321 gctcattttt gtcgaaacgc caatgctgca ctggccggac agcatgatga cttacctgac
     4381 aggcgacgcg gtgctattca gtaacgatgc tttcggtcag cactactgcg acgagcatct
     4441 gttcaacgat gaagtggatc agacggagct tttcgagcag tgccagcgtt actacgccaa
     4501 tatcctgacg ccgttcagcc gcctggtaac gccgaaaatt accgagatcc tgggctttaa
     4561 cttaccagtc gatatgatag cgacctctca cggcgtggta tggcgcgata acccgacgca
     4621 aattgtcgag ctgtacctga aatgggcggc ggattatcag gaagacagaa tcaccatttt
     4681 ctacgacacc atgtcgaata atacccgcat gatggctgac gctatcgccc agggtattgc
     4741 ggaaaccgac ccacgcgtgg cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga
     4801 aatcctgacc aatgtcttcc gctcaaaagg cgtgctggtt ggcacttcga cgatgaataa
     4861 tgtgatgatg ccgaaaatcg ccgggctggt ggaagagatg accggattac gcttccgtaa
     4921 caaacgcgcc agtgctttcg gctctcacgg ctggagcggc ggtgcggtgg atcgtctttc
     4981 cacacgcctg caggatgcag gtttcgaaat gtcgcttagc ctgaaagcca aatggcgacc
     5041 ggaccaggac gctctggagt tatgccgtga acacggtcgc gaaatcgccc gtcagtgggc
     5101 gctcgcgccg ctgccgcaga gcacggtgaa tacggtggtt aaagaagaaa cctctgccac
     5161 cacgacggct gacctcggcc cacggatgca gtgcagcgtc tgccagtgga tttacgatcc
     5221 ggcaaaaggc gagccaatgc aggacgttgc gccaggaacg ccgtggagtg aagtcccgga
     5281 taacttcctg tgcccggaat gctcccttgg caaagacgtc tttgacgaac tggcatcgga
     5341 ggaaaaatga gcaacggcat tgtgatcatt ggttcgggct ttgccgcccg ccaactggtg
     5401 aaaaatattc gcaaacagga cgccagtatt ccattaaccc tgattgccgc cgacagcatg
     5461 gatgagtaca acaaaccgga cctcagccat gttatcagtc aggggcaacg tgccgacgac
     5521 cttacccgcc agacggcggg tgaatttgcc gagcagttta atctgcgcct gtttccgcac
     5581 acctgggtaa cggatatcga tgccgaagcc catgtggtga aaagtcagaa taatcagtgg
     5641 caatacgaca agttagtgct ggcaactggt gccagtgcct ttgtcccgcc agtgcctggg
     5701 cgtgagttaa tgctgacgtt aaatagtcag caagagtatc gcgcctgtga aacgcaactg
     5761 cgggatgccc gacgcgtgtt gattgtcggc ggtggtttga ttggtagcga actggcgatg
     5821 gatttttgtc gtgcaggcaa agcggtcacg ctaatcgaca acgctgccag tattctggcg
     5881 tcgttaatgc caccagaagt aagcagccgc ttgcagcatc ggttgacgga gatgggcgtt
     5941 catctgctgt tgaaatctca gttgcagggg ctggaaaaaa cggattctgg cattctggca
     6001 acgctggacc gccagcgtag catcgaagtg gatacggtaa ttgccgccac cggactgcgc
     6061 ccggaaaccg ccctggcacg acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat
     6121 agttatctgc aaaccagtaa tgccgatatt tatgcgctgg gcgattgcgc ggaaattaac
     6181 ggtcaggtat tgccgttcct ccagccgatt caacttagcg cgatggtgct ggcaaaaaat
     6241 cttctcggca ataacacgcc gctgaaactc ccggcgatgc tggtgaaaat caaaacgccg
     6301 gaattacctc tgcatctggc aggcgaaacc cagcgtcagg atttacgctg gcaaattaat
     6361 accgaacgcc agggaatggt ggcgcgcggt gttgatgatg ccgaccagct tcgcgccttt
     6421 gtggtcagtg aggatcggat gaaagaggcg tttggattgt tgaaaacgtt gccggtatag
     6481 gcatgttttt ctccctcgcc caaagggcga ggggactgtc cgcgctctat ttacgcttag
     6541 ttacgccaca tccgccacta tgcagtatgc aggatgcgcg ccgcagcaat aaccccctgc
     6601 cccagagaca aaccgccatc acctgccggt aaactctgcg gaaagagtaa tgtgaaatca
     6661 gcgagataat gcgacagacg tgtacgcagc aatcggttat gaataacccc accgctaaat
     6721 accagcgtag tgataccgcg catcgtggcc tgctcacgca tcaacgcggc aaaaccctgc
     6781 gccagcgcat catgaaacgc ccacgcgcgt tgattaaccg gtgcctgcca gttcagccac
     6841 tgctgccaga aagtggcgag atccagttga ttgtccacca gcggcatcgt caccggatgc
     6901 gtcactccgt cgcacgaagc tgcgagcgcc tccagagcac aagccgcttc accttcataa
     6961 cttaacgtgg ctggcgcgca gcccagcgcc gccgccacag catcgaacaa acgcccacac
     7021 gatgacgcca gcggcgcgtt aattccacgc tcaatggccc gcgccagcac gctccagttt
     7081 tgctgttgca cacttgccgt ttcagggtaa ttctgccact ccggcacaaa gcgcaggcac
     7141 tgcgccagca ggttacgcca cggctgcttt gcagccaaat cgccccccgg aagcgccacc
     7201 gcaggcaagc cgcccaggtg ctcacattcg cgatagttca cccgcaggca ttcaccgccc
     7261 cacaaagcgc cgttctcccc cataccgata ccgtcgagcg tcaacgcaat gacatcaccg
     7321 ccatccagcg gccactgatg ctctgccaga cacgccgccg catgagcatg atgatgaagc
     7381 atcgtttgcg tcggcagatt catttcgcgc gcccactggc tggagacata gcccggatgc
     7441 acgtcatgca caacgtattg cggggtaaaa tcgtagatgt tttgcatcag gcgtaacgct
     7501 tcgcgccact gcatctggat gccatcgtca cttaaatcgc ccagatgctg actcaacacc
     7561 gcttgttcgc cgcgcaccag gcagaaggtg tttttcagat ccgcgccgag acacagcaca
     7621 ggcggaacat ttttaaagcc cggaggcaaa gccagcgcat ccggcacata cccccgcgaa
     7681 cggcgcagca tttcgccgct ttcgcgcact accgaatcat ccatccgctg cacgatgtcg
     7741 cggttatgta gcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg
     7801 ctgatagccg gtggtttacc gctcaggttg ccggaggtca tcaccagcgg acattgcagt
     7861 tcctgtaaca gcaaatgctg aagcggattc gcaggcaaca tcaccccaac ttcgttaagg
     7921 ccaggggcga tatcatcaca aagctcagga acgtattttt tatccaccag cacaatcggc
     7981 gcggcgggtg tggtaagcaa ctgacgggca gcgtctggta aaccttctgc cactggcaac
     8041 ataaccgcca gcggtttcgc cgggcgatgt ttgcgcgccc gcagtgtcgc caccgcgtta
     8101 ctgttacgcg catcgcaggc gagatgaaat ccgccaatcc ctttgatggc gacaatgttg
     8161 cccattttta actgtgcgat agctgcctgt aatgccgcct cttgttctgc atgttcacca
     8221 tgacttatcc attcaagatg tgggccacac tccgggcagg ccaccggctg ggcgtggaag
     8281 cgacgatcga gcgggtcgcg atattctttg tcacaggccg gacacagcgg aaacgccgcc
     8341 atcacggtaa acgggcgatc gtaaggcatc gcgcgaataa tggtgaaacg cgggccacag
     8401 tgggtacagt tgataaacgg ataacgccag cgtcgttcgc ctggggtatt catttcggca
     8461 aggcaagcag ggcaagtcgc ggcatcggga acaatttgcg tattcatgac gccacccgcg
     8521 ctctggcgga tggtgaactc agtgggcagt tgtgaccaga taaacggctc acgctcgacg
     8581 ctatcaatac gcgccagtgg cgggcagtgc tgatgcaatt gaacaagaaa cgtttccgga
     8641 tcttccagca gccggacttc tacgccatcg ccgtcattac agacatcgcc gtgaagattt
     8701 aattgctgtg ccagttgcca gacaaacgga cgaaaaccga cgccctgcac tttgccacga
     8761 atacgcagtt ggacaccgca agatgtgttt tttgccattg agttattccc gccatcatga
     8821 attgcgtaac ccgcccagcc ggacacgagc gcgtcgcatc cggcaatcac aggtcggcga
     8881 taccgcccgc tccgtattct acgaatattt ccgggaattc ctttgatgcc agaacagttc
     8941 agtaagattt ttagaacatc agcgccgtac ggcgacgttt ttctgcgctc agttgttcaa
     9001 gtttattacg atcgacacaa atcagcgcat gagtcgggca agccgccata cacgccgggc
     9061 cgtcttcacg atggttgcac aggtcacatt tattggcttc ggctttgtca gcccgtacat
     9121 tcagccccgc gccgctgttg cgaatcaccg gacgtaccac cacttccatc gcgccatacg
     9181 ggcaagccac aacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca tgaacaaacc
     9241 ctttatcacg gctgatagca ccattcgggc agacgttagc gcacggtgca tcttcacact
     9301 gacggcagac tgttgccgtg gaaatgttca cacctttaat gacatggatt cgcggtaaaa
     9361 aagtttccgg ggtcagcgat gcacagtcct gattctcctg atgagaaacc acgcacgcca
     9421 cttcacaggt acggcaacca atacatttac tcgcgtcagc aatgatgaaa cggttcatca
     9481 aattctccag caatgacagt taatgcgccg atacattcac aaatcatgcc agtttttaat
     9541 ttactgttat ttaaagaaat taaattctgt aatgcaggaa aaacgatgtc atcgacacta
     9601 gtgacgatga catgtgatga caatgtttat tgcgaaagag caatgaggga gccgcggcgg
     9661 atcagttttc cgctgaaggt tttcggcggt gagaaatccc cgccatcgag cataaaaatc
     9721 agccgtccaa taatttcctg aatcatctca gtcaccggaa tttttacgct ggagagcgcc
     9781 ggaacggtgt agggggcaat agcgatatca tcgaatccga taactgacac ctgctctggc
     9841 accgctacgc cgcgctcgtg taacgctttc atcgcaccta tcgccatatc gtcgttactg
     9901 gcaactaacg cgctaaattt agccccacgt tcgagcaacg tttctacccc ttcggccccg
     9961 ctggcgggcg tccatttacc gttagcgata agtttttcat tgagcgcaat accatgctgc
    10021 gccagcgcgt ctttataccc ggcaagacgt tcaacgctgg tgggggaatc catcgagccg
    10081 gtaaggaaag caatctcctg atgtccggcg tttatcaact ctgccacggc gttaaagctg
    10141 gtctgtttat gatcgcacca gacgctatgg ctgctgtttt tgcgcaggcg gcgattaagc
    10201 accattatcg gctgactgtg tgcgtcaatg atgtcatcaa tctcatccac gcttaaaaaa
    10261 cgcgggtaaa tcataatggc gtcgcagcgc aaatccagca gatactgaat cgcctggcgc
    10321 tcttcttcgg cgctgtgttt accatctgcc aatagcaact gtcgcccttt ctcttccgcc
    10381 attcgcgcgg catgaaacag taattcacta aaataaatgc cgtggtaaag cgtgttggtc
    10441 acgaccagcc ccagcgtctg agtactcttc gccgacagat tgcgcgccag caagtttgga
    10501 cggtaaccgc tctcttctac cgcctgaaac acgcgatctt tagtctcctg gctgacgtag
    10561 ccattacctg aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg cttcgccact
    10621 tccagcatcg tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt gtactgcacc
    10681 gtttgccgat tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg gtttcacaaa
    10741 tataaaaaat gaacaattaa ctctcttgct tattcagtga caactattca tgattttgtg
    10801 aaaccggttt cctaattccg tttcagcatc ggcatttttc cgtcacgtcg actgataaca
    10861 actacatcta ccctactgat aacaggataa aatccgatgg ccaaaaatta tgcggcgctg
    10921 gcacgctcgg tgatagcggc actgggcggc gttaataaca tctcggcagt cacgcactgt
    10981 atgacgcgtt tgcgctttgt tatcaaagat gaccagctta tcgacagtcc gacgttgaaa
    11041 accatctccg gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt gattatcggc
    11101 aataccgttt cacaagcctt tcaggaagtc gtcagcctgc tgccgggaga tatgcagccc
    11161 gcacagcccg tgggtaaacc caaacttacg ctacgtcgca taggtgcggg gatcctcgat
    11221 gcgctgatcg gcaccatgtc accgctgatc ccggcgatta tcggcggatc gatggtcaaa
    11281 ctgctggcga tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc gaccttaacc
    11341 attctgaatg tgattggtga cggtgctttc ttcttcctgc cgctgatggt cgcggcttct
    11401 gccgccatca aatttaaaac caatatgtcg ctggcgattg ccatcgcggg tgtgctggta
    11461 catccgagtt ttattgaatt aatggcgaaa gcggcccagg gtgaacatgt cgaatttgcc
    11521 ctgattccgg tcaccgcggt gaaatacacc tacacggtga tcccggcgct ggtgatgacc
    11581 tggtgcctgt catatatcga acgctgggtg gacagcatta caccggcggt gactaaaaac
    11641 ttcctcaagc caatgctgat tgtgttgatt gccgcaccgc tggcaattct gctgattggc
    11701 ccgattggta tctggatcgg tagcgccatt tcggcgctgg tttacaccat tcatggttat
    11761 ctgggctggc tttcagtcgc cattatgggt gcgctgtggc ctctgctggt aatgaccggg
    11821 atgcaccgcg tctttacgcc aaccatcatt cagaccattg ccgaaaccgg caaagaaggg
    11881 atggtcatgc cgtcagagat cggcgctaac ctgtcgctgg gcggttcatc actggcggtg
    11941 gcgtggaaaa cgaaaaaccc ggaactgcgc cagacggcgc tggctgcggc ggcatcagcc
    12001 attatggcgg ggatttccga accggcgtta tatggcgtgg cgatccgcct gaaacgtccg
    12061 cttatcgcca gtcttatcag cggttttatt tgcggcgcgg ttgccggtat ggcggggctt
    12121 gccagccact caatggcagc gccggggcta tttaccagcg tgcagttctt cgagccggcg
    12181 aatccaatga gcatcgtctg ggtgttcgcg gtcatggcgc tggcggtggt gctgtcgttc
    12241 atcctcacac tgttgctcgg ctttgaggat attcctgttg aggaagcgac tgccgaggcg
    12301 cgaaagcatc agagcgcaca accgaccgtc gccaaagaag taagtcttaa ttgaggatga
    12361 aaatgtcagt atttccagaa ggttttttat ggggcggcgc gcttgccgcc aaccagtctg
    12421 aaggtgcgta ccgtgaaggt ggcaaagggc tgaccaccgt cgatatgatc ccgcacggcg
    12481 agcatcgaat ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc gatgacgagt
    12541 tttatcccag ccatgaggcg acggattttt atcatcgtta taaagaagat atcgccctga
    12601 tggcagagat gggattcaaa gtgttccgta cctcaattgc ctggagccgc ctcttcccgc
    12661 agggcgatga actgacgccc aaccagcagg gcattgcctt ttaccgcgcg gtatttgaag
    12721 agtgtaaaaa gtacggtatc gaaccgctgg tcacgttgtg ccacttcgat gtgccgatgc
    12781 atctggtcac cgaatatggc tcctggcgta accgcaagct ggtggagttt ttcagccgct
    12841 acgcccggac ctgctttgaa gcatttgatg gcctggtgaa atactggctc accttcaatg
    12901 aaatcaacat tatgttgcat agcccgttct ccggcgcggg tctggtgttt gaagaaggcg
    12961 aaaatcagga tcaggtgaaa tatcaggctg cgcatcacca actgattgcc agtgcattag
    13021 ccaccaaaat cgcccatgag gttaacccgc aaaatcaggt gggctgtatg ctggcgggcg
    13081 gtaacttcta cccttacagt tgcaagccgg aagatgtctg ggcggcgctg gagaaagatc
    13141 gggaaaacct gttttttatc gatgtgcagg cgcggggcgc gtatccggct tactctgccc
    13201 gcgtattccg cgaaaaaggg gtaaccatcg acaaagcacc gggcgatgat gagatcctga
    13261 aaaacaccgt cgattttgtc tctttcagct attacgcctc acgctgcgcc tcggcggaga
    13321 tgaacgccaa caatagcagt gcggcgaacg tagtgaaatc gctgcgtaac ccgtatctgc
    13381 aagtgagcga ctggggctgg ggtattgatc cactcggtct gcgtatcacc atgaacatga
    13441 tgtacgaccg ctatcagaag ccgctgtttc tggtggaaaa cggcctgggc gcaaaagatg
    13501 aatttgcggc caatggtgag attaacgatg actatcgcat cagctattta cgcgaacata
    13561 tccgcgcaat gggcgaagcg attgcagatg gcattccgct gatgggctac accacatggg
    13621 gctgtattga tttagtttcc gcctctacgg gtgaaatgag caaacgctac ggttttgtct
    13681 ttgttgaccg tgacgacgca ggcaacggta cgctgacgcg cacgcgtaag aaatcgttct
    13741 ggtggtataa aaaagtgatt gccagtaatg gtgaggattt agagtaagta actaccgtcg
    13801 cacgaaccag gaaagcccga taagcgttgc gcatcgggca atcttgggtt tggcatcagt
    13861 caaaatcaaa gaaaccattc cggcttaaca ccaaatcggg ctgaaagtgc cttaatgtgg
    13921 gatatcgtca gggatcgttg cccagacaaa atctgactga ccagggattt agagccgatt
    13981 tcgttcttca aatccgcgta ggtcagatta tgttgatcaa tcagcgtacg aagtaacgcc
    14041 acgccaaccg gcattgctgc aacggctttg tcgaactcgg caaacttttc gttgttgttt
    14101 tcatattctg caatgcggct cgccagaaag tcgataagtg gattggtgtc atcttcttca
    14161 atcaaatatt cgaccagttc cagtgcttca cgataatcat cttctgagtc actacctccc
    14221 agaaagggga ctgcattgac cagttctctt gttgctttaa cagcgcgtgc agcattagca
    14281 gtcattcttt gttctctctg tagtaacgtg tcagtttgtc atactctgcg tgattcgtta
    14341 tgtgcttcac aaagaaccgc ttattaacaa agtttatgta ggcaataacc ctaagattgt
    14401 tcccgccgac atccagaacg taccatttgt tgcgatatct aaaattgtcg aggttgggaa
    14461 acgccgttcg catttcttct ggtgtagaaa aatccgtttc cttaataacc cgatacaatg
    14521 agtatagagc cagagcgtcg ttaggatatt tgcgcgcaca ctcttcaaat ggcgctttcg
    14581 aaatgatgtg cattttaact tccttattag cgcctgaatg actaaacctg gactaccatt
    14641 ccttcggatt tattttccta tagtagttga tcacacgggt gttcacaata tgtaaacacc
    14701 cgtctacacg tcacaattgc acgtccaaat tccaaaacgt gaacatgttt gtctacgaac
    14761 tactcttctt ccaccgctaa ctgcgcgaat ccgccattcc cttcccagcc ttccagtcgt
    14821 tgataaacgg tttctaccgc atctttaatc ggctgggtca tcgggtagta aaagccgacg
    14881 atatccggct ggatgccgag gaaaatcact tcgccaatgt cttcttttaa ctggtcgata
    14941 aggtagttca acggcatgtt atgggtagtc atcataaaca tctcggcgat atcatccgga
    15001 tcgatgatgc ggatctcgcc ggggtttagc cccatatccg tggcgtcgac aatcagcagt
    15061 cgtgtcgggc gcagttcgcg aatagcgaca atgtcgtttt ccggtgcgct accgccgtca
    15121 atcaccaccc agttaccttt cggcgcggcg gcgcactttt ccgccagcag cggacctgcg
    15181 ccatcatcgc ccatcatgct attgccaaca cagagtaaaa cgtcagtcac gcaatctcct
    15241 caccatcaaa tagatggcgt tttcctgatg aatatcatgc agcatactta acagcattgt
    15301 actccattcc ttctgctgcg aagtttgcgc agcccgggct ttatcgaatg cgttggccag
    15361 catcggcaca tggttaatgt cgatgacgat ctcaccatat ttcggcacgc cttccatttt
    15421 gcggcgggct tcgctgcccg cctccagagt ggcaatccat gccagatatt catcccacgg
    15481 acaggtgagc gccgcttcca ggcaatcgat aaccccaagg tggtgaccaa tcgccaggct
    15541 gtaatagacc acctgctgcg cctcggcggg cgtggcatcg ttctcatcaa taaatttacg
    15601 gctcagttga ctgaacacca ccttttcact catcggatac gcgcctcttc aacaacatga
    15661 ttcagatggc tgacaatctc gttcagacgc ggatcgtttt ccgcttccag ccagcgtgca
    15721 acctgctctt cgccctgccc taactgtgtc aggaagtcat cggcaatctg gcgaccgtaa
    15781 cggtaacccg ccaggcggcg agcttcgcga tccactttca cgcgcagcgg ctgcaccata
    15841 tcgccatgca ggatctccgc cggttgttca tcctgctcac ccggcccacg ggcgtgaatt
    15901 ttctgctcca gcaggccgag cgccattgca aagccgtaca gcgtggcggc aggcgttggc
    15961 gggcagccag ggatataaac atccaccggg acgattttat ccgtaccgcc ccacacgcag
    16021 tagagatcgt ggaagatccc gccgctgtta ccgcaggcac cgtaggagat acaaattttc
    16081 gggtccggcg cggactgcca cgcacgcagc gcaggggatc gcattgcacg ggtgaccgcg
    16141 ccggtaaaca gtaaaatatc cgcatgacgc ggtgaaggaa cgactttaat gccgaagcgt
    16201 tctgcatcaa acagcggcga aagcgtggcg aaaatttcga tttcgcagcc gttgcagccg
    16261 ccgcagtcca cgcggtaaac gtaggcagaa cgtttgattt ttttcagtaa cgacgccttc
    16321 atgctggcga tggattcatc caccgtcatc gggaccggaa tgccgttggc gtcacggggg
    16381 cctaataaat tgctcatcag atggcctctt tcatatggcg agtcagttca atacggtcgg
    16441 acggcaccag gcatttctgg cgcttacatt ccgggcaagt ctcaaagctt tcgcggtggt
    16501 tttccgcgcg gctgtcgccg ttgtgcttga gcagcgcaat ggcgtagtcg atctctttct
    16561 ggacggcgaa aggacgattg cagacgcgac agttgcacag cgcgaagcgg gactgttgca
    16621 ggaagtcttc tttcttccac accgccagtt cgtactcttg cgacagctta atcgccgccg
    16681 tcgggcaaac ttcttcgcag cgtccacaga agatgcagcg cccgagatta aactcccagg
    16741 caagctctcc tgtggcgagg tcagtttcaa ccgttaaggc gtttgacggg caggcattga
    16801 cgcaggccgc gcagccgatg cactgctgcg ggttctgctc tggcttacca cggaagtttt
    16861 tatcaaccgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgataactt
    16921 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    16981 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacca
    17041 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    17101 cggaaacggt gttgccgcgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    17161 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    17221 cgccgcgcgg cgcttcggca aagcccagcg caaaacggtg cggaatatag gtaaagcctt
    17281 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcatg ttcagcgcgg
    17341 tatagacctc gttgatacgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    17401 ggacttccat tggcagcagg ccataaccaa caaacgggtg atcggcacgg gtatcgcggg
    17461 cgtgaccgct ggcgcggacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    17521 ccagacgacc aatgccgaca gtacgctgtt ccatgttcgg tgtgctcagc agcacatcca
    17581 ccagctcctg cacttcacga cgcatctgtt gcgccaactg gcgggtctgg atcatgtcat
    17641 ctttcagcag atcgcgacga attccgccga tcaggttcag gccgtaggtt ttacgcgcac
    17701 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    17761 cggagtcgaa gccggtaaag tgactggcga ggccgaggtt gagcagatgc gagtgcaggc
    17821 gttctacctc cagcagaatg gcgcggatca tctgcgcacg ttctggcacc tgaataccca
    17881 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgagc aaagccgcag atcccgcaca
    17941 cacggtcaga gaggaaggtc acttcgttgt aacccatgcg ggtttccgcc agtttttcca
    18001 tgccgcgatg gacatagaac aggcggtagt cggcgtcgat aatgttttcg ccatcgacga
    18061 acagacggaa gtgacccggt tcgtcggaag tgacgtgcag cggaccaatc ggcacgacgt
    18121 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    18181 ccggacgttg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    18241 catccggcag caccagacga cgttcatccg gcagccctac cggaatcaaa ccgtacatat
    18301 cgcgcacttc acgctcgccc cataccgccg ccggaacgcg cggcgttacg gacggatatt
    18361 ccggtttgtt ggcgtcaact tcaacgcgca cggttatcca gcatttggtg cccttctcca
    18421 tcgacagcac gtagtaaacg gcgtaatgac cattaagttt gcgttcgtcg ttaccaaaca
    18481 gcaccgacag ccagcccccc tgcttgtagt agagaaactc caccacttcc ggcaggtagt
    18541 tcaccttcac ggtcacggtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    18601 ccggaaatgc ctcattcagc gcagcgagat aatgttgacc taatttttct tcagacatgc
    18661 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    18721 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    18781 cagcgcgata accaggacgc caaccaccag tttgacgatg gcaatcacca gcgccagcag
    18841 cagcccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    18901 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctgcc
    18961 gctatactca gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    19021 cagtttgccc atttcgataa aggtggcgaa tgcacaggca caaagcgcca gcaccagcgg
    19081 aatgctctgg ctcagcggcc agtgataaac ggtgtcggtg atgttgctga tgttggtgga
    19141 accggcaacc tgtgcggcga cccacagacc aagcagcagc atcggttcaa ccagcacgcc
    19201 aagcatcgct tcacggctcg cgccgatagc ggtaaacggg ctaccggtat ccagaccaga
    19261 aatggcaaag aagaaacgcg cgatggcaaa gagatacagt aaagtgatca aatcgcccag
    19321 ctgcggcagc ggagaaccaa cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    19381 gcccaccatc acatacggcg tcaggcggaa cacccagccg gaggcatccg ggccaacgct
    19441 ctggcgaccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    19501 gttatgcaag cgggcgcgcg ccacgcgggt tataccggag agcagcggcg caacggcaaa
    19561 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    19621 ataatcacca ccagcaccgc cagttcaacc agcgccatcc ggcggaacag caacgcactg
    19681 ccttcgcact gccagcccgg caccagcgac accggattca gccatttgcg cagtttcagc
    19741 actggcgcaa acgcctgttt caccggcatg gcaaaaccgt gagcggtaat caccattgat
    19801 ttttcgtggt cgtagccgca cacccaggcc gcgccgcggg aacgcgacgg caaacggtcg
    19861 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaacgtg
    19921 atcatgggtt gagaaacggt ggtgttagca ggctccagcg gcagaggtac agcagcagag
    19981 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    20041 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    20101 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc tagcgcaccg
    20161 gtaattgcca gacccactgc gagcagcggc cccagcagac gggcaacaaa cgcgccacta
    20221 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagcggc
    20281 ggcaacgcag ccattgccat cagcccaact aacatggcga tggagataac cggcattttc
    20341 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    20401 gccccgagga aaagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    20461 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    20521 agccccagca ggataatgcc gatattttcc agggtgtggt aagccagcag gcgctggatg
    20581 ttgtgttcca tcaacgcata cagaccaccg acgaacgcgg tgatcatgcc gagcaccagc
    20641 agcgcgattc cccaccacag cggtgcatta ccgcccagca gtgacagggt taaaatgccc
    20701 agcaggccaa ttttcatgac taccgtggaa aacaacgcgg cagctggcgc agaggcgtta
    20761 gcatgtgcct gcggcaccca gccgtgcagc ggaataatcc cggccagcag gccaaagcca
    20821 atcacaccga gcagccagat atcggaaccg agcggcagct gttgcatacg catatccagc
    20881 aggcgcagat ccagcgtgcc gtaacgctgc cacagcagcc agcaagcaat cgccagcagc
    20941 agagtgccaa gacgccccag tgcaaaccac agtttgccct ctttgctgtt gttggtgagg
    21001 aacaccgcgc acaaggccat gatttctgcc attaccacga acataccgag gttgctggcg
    21061 atgacagcac agacggcagc agccatcaac atattgatct gcaagccgtt gcacttcacc
    21121 tgcgcgtggc gatgccagtc aatgttgtag aggctgacaa acagaccgca caggccgagc
    21181 gtaatcagcc aaatcgcgtt aagcggagag atttgcacat cgtagcttac tagcgacagc
    21241 gcaccgctca cgccaaccgc gccagtcagt acagtgaagc ccgcggctgc cgtatacaga
    21301 ctacccaccg cgccgccaat tccggctatc cagccgctta gcgccttttg aaaagaaaag
    21361 agaaatgcca gaacagcggc ggcgacaaac cacgccacgc cgctattgat cagggaaatt
    21421 gcgctcattt agcctctcca ctttgagcct gctgaaacaa ggtgagatcg ccaaagtccg
    21481 tgttaaaggt cagctcacgc ttacgtttgc tgacgcgggc gatatcggtg ttatccacca
    21541 gatgcagggc tttagtcggg cacatccgca cgcaggccgg accttgttca tcaaagctac
    21601 aaaggtcaca tttgacggcg atcgcgcgaa tacctggcac ccagtcaagc aatgtgctga
    21661 cacgcgccgg agcaggcggt gccggtggcg ctttcggggt attggcgttt gccggaatat
    21721 ccagcggacg gctgccggaa aattcaattg cgccaaacgg gcaggcgatg ccgcacagtt
    21781 tgcagcttac gcacaggctt tcattcaact gcacggcccc atcaacgcgg gtgatggcgt
    21841 taaccgggca gaccaccgca cagggcgcat cttcacagtg gtgacagagc tgcggcgcag
    21901 attctttttc attgagcatc actcgcaggc gcggcattga ttgcaggccg tgctggcgat
    21961 gcgtctctga acaggcggcc tcacaggtgt ggcagccgat acagagcgtg gagtcagcaa
    22021 ttacaaaacg attcaccagg cattcctcag gtgattgtca tttttgacga aaacatgccg
    22081 atgaaatgtc attttcgaca ctcatcgaca cgcccatccc caaacaggcg taacgcctgc
    22141 aaaacgggca aagcctcagc tcatgctgcc gggctttgtc cctttaccag ttggcttaaa
    22201 ttcaccggca cattgttttc aatggcggtg tataagctgt cgtaaacgcc agcgattttt
    22261 tcgagatagt gttccagaac ctgttcgcga tcgcggatgt tgaccttgcc atcaatcatg
    22321 ccgtcaatgc tcagttgcgc ctgcgcaatc cgctgtttgt cttcaccatc ttttgtttcg
    22381 acgtaatact cgatggtgtg gctgttaaag ccatccgcca gggtgacgta aatgcgaaaa
    22441 tgttcaaaaa ggacgaaaca gagttctttg tccggcgcgc agctcatggc gtgatgcagg
    22501 cgcgctttcg ataacgtgat cccctgaacc agcgaattgc agtagatgtg ccactggtcc
    22561 tgtaggcgac gatgccgctg tgcgatgtaa tcggctttct cgcttatttc ccaaatagtc
    22621 attgtcaggt tacccgttta acagagatgc cagctttaag cattttttgt gccaactttt
    22681 aatttattgt tattaaagcg atttttaagc taaagatgaa tttcgtcgcc gtgtcgacgt
    22741 gtcatttcga catcatcgac attattcacc gcagggataa tcaacactgg cacaattatt
    22801 gcttgtagct ggcaatagtt aatgggaggc gatatgcacg aaataaccct ctgccaacgg
    22861 gcactggaat tgatcgaaca gcaggccgca aaacacggcg caaaacgcgt aactggggtc
    22921 tggctcaaaa ttggcgcatt ttcttgtgtc gaaaccagct ctcttgcctt ttgttttgat
    22981 ctggtttgcc gcggcagcgt ggcggaaggt tgtaaactgc acctcgaaga acaagaggcc
    23041 gaatgctggt gtgaaacatg ccaacagtat gtgacgctac tgacccagcg cgtccgccgc
    23101 tgtccacagt gtcatggtga catgctacag attgtggcag acgacggttt acagattcgg
    23161 cggatagaaa tagaccagga gtgagcgatg tgtacaacat gcggttgcgg tgaaggcaac
    23221 ctgtatatcg agggtgatga acataaccct cattccgcgt ttcgtagcgc gccatttgcc
    23281 ccggcggcac gcccgaagat gaaaatcacc ggcattaaaa cgcctgaatt tacccccagc
    23341 cagactgaag aaggcgacct gcattacggt catggcgaag cgggcactca cgcgccgggc
    23401 atgagccagc gtcggatgct ggaagtcgaa attgacgtgc tggacaaaaa taaccgtctg
    23461 gctgaacgca accgcgcgcg ctttgctgcc cgcaagcaac tggtgctcaa cctggtttcc
    23521 agccctggtt ccggtaaaac caccctgctg acggaaacct taatgcgcct gaaagacagc
    23581 gttccgtgcg cggttattga aggcgatcag caaaccgtga acgatgccgc acgcattcgc
    23641 gctaccggca caccagcgat tcaggtgaat accggtaaag gctgccatct tgacgcacag
    23701 atgattgccg acgccgcacc gcgcctgcca ctggacgata acggtattct gtttatcgaa
    23761 aacgtcggca acctcgtgtg cccggccagc ttcgatctcg gtgaaaaaca caaagtggcg
    23821 gtgctttccg ttaccgaagg tgaagacaaa ccgctgaaat atccgcatat gtttgccgca
    23881 gcttcgctga tgctgctcaa caaagttgac ttgctgccgt atctcaactt tgacgttgag
    23941 aagtgcatcg cctgcgcccg cgaagtcaat ccagaaattg aaatcatcct tatttccgcc
    24001 accagcggcg aagggatgga ccagtggctg aactggctgg agacacagcg atgtgcatag
    24061 gcgttcccgg ccagatccgc accattgacg gcaaccaggc gaaagtcgac gtctgcggca
    24121 ttcagcgcga tgtcgattta acgttagtcg gcagctgcga tgaaaacggt cagccgcgcg
    24181 tgggccagtg ggtactggtt cacgttggct ttgccatgag cgtaattaat gaagccgaag
    24241 cgcgcgacac tctcgacgcc ttacaaaaca tgtttgacgt tgagccggat gtcggtgcgc
    24301 tgttgtatgg cgaggaaaaa taatgcgttt tgttgatgaa tatcgctcgc cggaacaggt
    24361 gatgcagtta attgagcatc tgcgcgaacg tgcttcacat ctctcttaca ccgccgaacg
    24421 ccctctgcgg attatggaag tgtgcggtgg tcatacccac gccattttta aattcggcct
    24481 cgaccagttg ctgccggaaa acgttgagtt tatccacggt cccggttgcc cggtgtgtgt
    24541 actgccgatg ggtagaatcg acacctgcgt ggagattgcc agccatccgg aagtcatctt
    24601 ctgtaccttt ggcgacgcca tgcgcgtgcc ggggaaacag ggatcgctgt tgcaggcaaa
    24661 agcacgcggt gccgatgtgc gcatcgttta ctcgccgatg gatgcgttga aactggcgca
    24721 ggagaatcca acccgcaaag tggtgttctt cggcttaggt tttgaaacca ccatgccgac
    24781 gacggccatc actctgcaac aggcgaaagc gcgtgatgtg cagaattttt acttcttctg
    24841 ccagcatatt acgcttatcc cgacgctgcg cagtttgctg gaagagccgg ataacggtat
    24901 cgacgcgttt cttgcgcccg gtcacgttag tatggttatc ggcactgatg cctataattt
    24961 tatcgccagc gattttcatc gtccgctggt ggtcgctggt ttcgaacccc ttgatctact
    25021 gcaaggcgtg gtcatgctgg tggagcagaa aatagcggcc cacagcaagg tagagaatca
    25081 gtatcgtcgg gtggtgccgg atgccggtaa cctgctggcg caacaggcga ttgccgatgt
    25141 gttctgtgtc aacggcgaca gcgaatggcg cggcttaggc gtgattgaat cttctggtgt
    25201 acacctgacg ccggattatc aacgattcga tgccgaagcg catttccgcc cggcaccgca
    25261 gcaggtctgc gatgacccgc gcgcacgttg tggcgaagta ttaacgggca aatgtaagcc
    25321 gcatcaatgc ccgctgtttg gtaacacctg taatcctcaa accgcgtttg gtgcgctgat
    25381 ggtttcctcc gaaggagcgt gcgccgcgtg gtatcagtat cgtcagcagg agagtgaagc
    25441 gtgaataata tccaactcgc ccacggtagc ggcggccagg cgatgcagca attaatcaac
    25501 agcctgttta tggaagcctt tgccaacccg tggctggcag agcaggaaga tcaggcccgt
    25561 cttgatctgg cgcagctggt agcggaaggc gaccgtctgg cgttctccac cgacagttac
    25621 gtcattgacc cgctgttctt tcctggcggt aatatcggca agctggcgat ttgcggcacc
    25681 gcgaatgacg tagcggtcag tggcgctatt ccgcgctatc tctcctgtgg ctttatcctc
    25741 gaagaaggat tgccgatgga gacactgaaa gccgtagtga ccagcatggc agaaaccgcc
    25801 cgcgcggcag gcattgccat cgtcactggc gatactaaag tggtgcagcg cggcgcggca
    25861 gataaactgt ttatcaacac cgctggcatg ggcgcaattc cggcgaatat tcactggggc
    25921 gcacagacgc taaccgcagg cgatgtattg ctggttagcg gtacactcgg cgaccacggg
    25981 gcgactatcc ttaacctgcg tgagcagctg gggctggatg gcgaactggt cagcgactgc
    26041 gcggtgctga cgccgcttat tcagacgctg cgtgacattc ccggcgtgaa agcgctgcgt
    26101 gatgccaccc gtggtggtgt gaacgcggta gttcatgagt tcgcggcagc ctgcggttgc
    26161 ggtattgaaa tttcagaagc ggcactgccg gttaaacctg ctgtgcgcgg cgttaccgaa
    26221 ctgctgggac tggacgccct taactttgcc aacgaaggca aactggtgat cgccgttgaa
    26281 cgcaacgcgg cagagcaagt gctggcagcg ttacattccc atccactggg aaaagacgcg
    26341 gcgctgattg gtgaagtggt ggaacgtaaa ggtgttcgtc ttgccggtct gtatggcgtg
    26401 aaacgaaccc tcgatctacc gcacgccgaa ccgcttccgc gtatatgcta ataaaattct
    26461 aaatctccta tagttagtca atgacctttt gcaccgcttt gcggtgcttt cctggaagaa
    26521 caaaatgtca tatacaccga tgagtgatct cggacaacaa gggttgttcg acatcactcg
    26581 gacactattg cagcagcccg atctggcctc gctgtgtgag gctctttcgc aactggtaaa
    26641 gcgttctgcg ctcgccgaca acgcggctat tgtgttgtgg caagcgcaga ctcaacgtgc
    26701 gtcttattac gcatcgcgtg aaaaagacac ccccattaaa tatgaagacg aaactgttct
    26761 ggcacacggt ccggtacgca gcattttgtc gcgccctgat acgctgcatt gcagttacga
    26821 agaattttgt gaaacctggc cgcagctggc cgcaggtggg ctatacccca aatttggtca
    26881 ctattgcctg atgccactgg cggcggaagg gcatattttt ggtggctgtg aatttattcg
    26941 ttatgacgat cgcccctgga gcgaaaaaga gttcaatcgt ctgcaaacat ttacgcagat
    27001 cgtttctgtc gtcaccgaac aaatccagag ccgcgtcgtc aataatgtcg actatgagtt
    27061 gttatgccgg gagcgcgata acttccgcat cctggttgcc attaccaacg cggtgctttc
    27121 ccgcctggat atggacgaac tggtcagcga agtcgccaaa gaaatccatt actacttcga
    27181 cattgacgat atcagcatcg tcttacgcag ccaccgtaaa aacaaactca acatctactc
    27241 cactcactat cttgataaac agcatcccgc ccacgaacag agcgaagtcg atgaagccgg
    27301 aaccctcacc gaacgcgtgt tcaaaagtaa agagatgttg ttgattaatc tccacgagcg
    27361 ggacgattta gccccctatg aacgcatgtt gttcgacacc tggggcaacc agattcaaac
    27421 cttgtgcctg ttaccgctga tgtctggcaa caccatgctg ggcgtgctga aactggcgca
    27481 atgcgaagag aaagtattta ccactaccaa tctgagctta ctgcgccaga ttgccgaacg
    27541 tgtggcaatc gctgtcgata acgccctcgc ctatcaggaa attcatcgtc tgaaagaacg
    27601 gctggttgat gaaaacctcg ccctgaccga gcagctcaac aatgttgata gtgaatttgg
    27661 cgagattatt ggccgcagcg aagccatgta cagcgtactt aaacaagttg aaatggtggc
    27721 gcaaagtgac agtaccgtgc tgatcctcgg tgaaactggc acgggtaaag agctgattgc
    27781 ccgtgctatc cataatctca gtgggcgtaa taatcgccgc atggtcaaaa tgaactgcgc
    27841 ggcaatgcct gccggattgc tggagagcga tctgtttggt catgagcgtg gggcttttac
    27901 cggtgccagc gcccagcgta tcggtcgttt tgaactggcg gataaaagct ccctgttcct
    27961 cgacgaagtg ggcgatatgc cactggagtt acagccgaag ttgctgcgtg tattacagga
    28021 acaggagttt gaacgtctcg gcagcaacaa aatcattcag acggacgtgc gtctaatcgc
    28081 cgcaactaac cgcgatctga aaaaaatggt cgccgaccgt gagttccgta gcgatctcta
    28141 ttaccgcctg aatgtattcc cgatccacct gccgccacta cgcgagcgtc cggaagatat
    28201 tccgctgctg gcgaaagcct ttaccttcaa aattgcccgt cgtctggggc gcaatatcga
    28261 cagcattcct gcggagacgc tgcgcacctt gagcaacatg gagtggccgg gtaacgtacg
    28321 cgaactggaa aatgtcattg agcgcgcggt attgctaaca cgcggcaacg tgctgcagct
    28381 gtcattgcct gatattgctt taccggagcc tgaaacgccg cctgccgcaa cggttgtcgc
    28441 ccaagagggc gaagatgaat atcagttgat tgttcgcgtg ctgaaagaaa ccaacggcgt
    28501 ggttgccggg cctaaaggcg ctgcgcaacg tctggggctg aaacgcacga ccctgctgtc
    28561 acggatgaag cggcttggaa ttgataaatc ggcattgatt taacagatgt ggttatactc
    28621 gatcaacatt caatgaaagg aggcagtatg gcagtttcag caagaaatca attaaccgga
    28681 acagttagcg cagtagcaat gggcgctgtg aatgatgaag tagaactgac gctggcaggt
    28741 ggtgcaaagc tggtggcaat tgtgactcac agcagccagc aagcgctggg tctggcgaaa
    28801 gggaaagaag cgattgccct gattaaagcc ccgtgggtaa cgctggcaac ggaaggctgt
    28861 ggactgaaat tctctgctcg caaccagttt gctggcagcg tgtcgacaat caccgaaggc
    28921 gcagtgaacg ccactgtcca tatcaaaacg gatgcgggtt ttgaaattgt ggcagtggta
    28981 acaaatgaaa gtcaggatga aatgaaactc accactggct cgcgcgttat tgcattgatc
    29041 aaagcatcag ccatcctgat tgccaccaaa gcgtaaacgc cttatttgcc ggagagtatg
    29101 gctctccggc agatttattt ctttggacga tatttttctg gcaactccgg caccggacgc
    29161 ttgtcatcga tgagatgacg cacggttaag atcggatgac gccacagcat tctcggcccg
    29221 gcccaacgca taatctgttt catctcttca cgctttgcag gctggtaaca gtgcaccgga
    29281 cactgcttac aggctggttt ctcttcgccg aacacacatt tatccagccg cttttgcgcg
    29341 taggcaaaca gcgcgtcgta atgctccggc tccgctgacg cctgcgggca tttcgcttga
    29401 taaagatcga tcattttttt aatcgtcagt ttttcacgag agatacgctt gccggacatg
    29461 ctgcctccat actcttaagg tgtatttata ttacaactta atttgaaagg ggactatggc
    29521 tctgaaggag gaggtttatt caaccgacgc aattttgcgt acctgcttca taagcatcac
    29581 gcaaaagctg caaaacagca tctttcccgg aatcaccatc aagaactcgc cgttcgcttc
    29641 ttcccctgaa atgattaact ccggtatcat gtgcgcctta tgtgattaca acgaaaataa
    29701 aaaccatcac acctcattta atatcaggga accggacata accccatgag tgcaatagaa
    29761 aatttcgatg cccatacgcc catgatgcag cagtatctca agctgaaagc ccagcatccc
    29821 gagatcctgc tgttttaccg gatgggtgat ttttatgaac tgttttatga cgacgcaaaa
    29881 cgcgcgtcgc aactgctgga tatttcactg accaaacgcg gtgcttcggc gggagagccg
    29941 atcccgatgg cggggatccc ctaccatgcg gtggaaaact acctcgccaa actggtgaat
    30001 cagggcgagt ccgttgccat ctgcgaacaa attggcgatc cagcgaccag caaaggtccg
    30061 gttgagcgca aagttgtgcg tatcgttacg ccgggcacca tcagcgatga agcgctgttg
    30121 caggagcgtc aggataacct gctggcggct atctggcagg acagcaaagg tttcggctac
    30181 gcgacgctgg atatcagttc cgggcgtttt cgcctgagcg aaccggctga ccgcgaaacg
    30241 atggcggcag agctgcaacg cactaatccg gcagagctgt tgtatgcaga aaatttcgcc
    30301 gagatgtcgc tgattgaagg tcgtcgcgga ctgcgccgtc gcccgctgtg ggagtttgaa
    30361 atcgacaccg cgcgccagca gttgaatctg caatttggca cccgcgatct ggtcggtttt
    30421 ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt gtctgttgca gtatgcgaaa
    30481 gatactcaac gcacgaccct gccgcatatt cgttctatca ccatggaacg tgagcaggac
    30541 agcatcatta tggatgccgc gacgcgtcgt aacctggaaa ttacccagaa cctggcgggc
    30601 ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg tcacgccgat gggcagccgt
    30661 atgctgaaac gctggctgca tatgccagtg cgcgataccc gcgtcttgct tgagcgccag
    30721 caaactattg gcgcattgca ggatttcacc gccgagctgc agccggtact gcgtcaggtc
    30781 ggcgacctgg aacgtattct ggcgcgtctg gctttacgaa ctgctcgccc acgcgatctg
    30841 gcccgtatgc gtcacgcttt ccagcaactg ccggagctgc gcgcgcagtt agaaaatgtc
    30901 gatagcgctc cggtacaggc gctgcgtgaa aaaatgggcg agtttgccga gctgcgcgat
    30961 ctgctggagc gagcaatcat cgacacaccg ccggtgctgg tacgcgacgg tggtgttatc
    31021 gcaccgggct ataacgaaga gctggatgag tggcgcgcgc tggctgacgg cgcgaccgat
    31081 tatctggagc gtctggaagt ccgcgagcgt gaacgtactg gcctggacac cctgaaagtc
    31141 ggctttaacg cggttcatgg ttattacatt cagatcagcc gtggacaaag ccatctggca
    31201 cccatcaatt acatgcgtcg ccagacgctg aaaaacgccg agcgctacat tattccagag
    31261 ctaaaagagt acgaagacaa agttctcact tcaaaaggca aagcactggc actggaaaaa
    31321 cagctttatg aagagctgtt cgacctgctg ttgccgcatc tggaagcgtt gcaacagagc
    31381 gcgagcgcac tggcggaact cgacgtgctg gtgaacctgg cggaacgggc ctataccctg
    31441 aactacacct gcccgacctt tattgataaa ccgggtattc gcatcaccga aggccgccat
    31501 ccggtggttg aacaggtgct gaatgagccg tttatcgcca acccgctgaa cctgtcgccg
    31561 cagcgtcgca tgttgatcat taccggtccg aatatgggcg gtaaaagtac ctatatgcgc
    31621 cagaccgcac tgattgcgct gatggcctac atcggcagct atgtaccggc acaaaaagtc
    31681 gagattggac ctatcgatcg catctttacc cgcgtagggg cagcggatga tctggcatcc
    31741 gggcgttcaa cctttatggt ggaaatgacc gaaaccgcca atattttaca taacgccacc
    31801 gagtacagtc tggtactgat ggatgagatt gggcgcggga cgtccactta cgatggtctg
    31861 tcgctggcgt gggcgtgcgc ggaaaatctg gcgaataaga ttaaagcgtt gacgttgttt
    31921 gccacccact atttcgagct gacccagtta ccggagaaaa tggaaggcgt cgccaacgtg
    31981 catctcgatg cactggagca cggcgacacc attgccttta tgcacagcgt gcaggatggc
    32041 gcggcgagca aaagctacgg cctggcggtt gcagcccttg cgggcgtgcc aaaagaggtg
    32101 atcaaacgcg cgcggcagaa actgcgtgag ctggagagca tttcgccgaa tgccgccgcg
    32161 acgcaggtag atggcacgca gatgtctttg ctgtccgtac cagaagaaac ttcgcctgcg
    32221 gtcgaggcac tggaaaacct cgacccggat tcactgactc cgcgtcaggc gctggagtgg
    32281 atttatcgct tgaagagtct ggtgtaataa caattcccga tagtcttttg ctatcgggaa
    32341 tattaacgac aactgacgaa tcaaataaaa acaccctgta taataggaaa tcttatttta
    32401 cagggtaaaa ccatgccatc tacacgctat caaaaaatta atgcccatca ctatcgttat
    32461 atatgggccg ttggtgatat tcatggtgac tatcaattat tacaatcccg cttacatcaa
    32521 ctctcttttt tccctgaaac cgacttactt atttctgtcg gcgataatat tgatcgtggg
    32581 ccggagagtc ttaacgtact acgcctgtta aaccagccct ggtttacatc ggttaaaggc
    32641 aaccatgaag cgatggcgct tgatgcattc gaaactggcg atggaaatat gtggcttgcc
    32701 agcggcggcg actggttctt tgaattaaat gagacggaac aacaagaagc aataaatctg
    32761 ttgctgaaat ttcatcgcct tccacatatt attgaaatca ctaacgacat aataaaatat
    32821 gtcatcgcac atgcagatta tccggggaat gaatatctct ttggcaaaga aatagcggag
    32881 agagaactac tctggcctgt tgatcgtgtg cagaaatcgc ttaatggcga gttacaacaa
    32941 ataaacggcg ctgattattt tatatttgga catatgatgt ttgataacat tcagacgttc
    33001 gcaaaccaga tttatattga taccggatcg ccgaaaagcg ggcggctgtc attttataaa
    33061 ataaagtagt ctcatgcttc ttctgtgaag catgagtaac ccggtgttat tgcaggccat
    33121 tattcatttt tcgctaccag caaagagaga tcctgcttcg ccagcgcgcg actggcactc
    33181 tccggcaaac cgtcgtctgt aataatctga tcaaactcgc ttaatggtaa cgccagccat
    33241 gtcgccacct gaccatattt cgtcgcatca cagaccaaaa ctcgctggcg gctggcactg
    33301 gcaatcgccc gtttcaccgt gactttatct tccgctggcg tagaaatccc ccgcacactc
    33361 catgacgatg cagaaataaa agcctgatca atcatcaggc tgcgcagcat ggtcgcagcg
    33421 gcttccccga cacaggaacg gttttcccga cacactgcac cgccagtgtg aataattgtg
    33481 caattactgt tgtcgagcaa gtagtccgca ataacgaaat cgtttgtgac cacagtcagt
    33541 gactccatgt gaatcagatg ctgtgctatc gctagcgtgg tcgttcccgc atccagatag
    33601 atacaacttc ccggctgaac aagacttgcc gccagcttgc caatagccgc tttttgcgtc
    33661 attgccagcg cagtttttac ctgatgagaa ggttcatgcg ccacgcgtcc cggagactgg
    33721 acgcctccgg acaccagcac aacggctccc tgctgctcca gtttttgtaa atcccgacga
    33781 atggtcatat gtgacacatt cattctgtcc gttagttcag caatactgac aatgcctttt
    33841 tcagctacca tctcaaggat gatttggcga cgctctacgg gtatcaactt ttgctccttc
    33901 ctttgtcctg ctgacattct acgctatttg cctgcgaaac gtgcgcggcg caactaacgc
    33961 ttagttcaca taaaataaca cacaatgtta atttttgtga atcagctcac catatcgtta
    34021 tcttccagcg cctatattca caatatcaaa caaaatatca cttaaattaa caaggagagc
    34081 agatgaaaac gggatctgag tttcatgtcg gtatcgttgg cttagggtca atgggaatgg
    34141 gagcagcact gtcatgtgtc cgcgcaggtc tttctacctg gggcgcagac ctgaacagca
    34201 atgcctgcgc tacgttgaaa gaggcaggtg cttgcggggt ttctgataac gccgcgacgt
    34261 ttgccgaaaa actggacgct ctgctggtgc tggtggtcaa tgcggcccag gttaaacagg
    34321 tgctgtttgg tgaaacaggc gttgcacaac atctgaaacc cggtacggca gtaatggttt
    34381 cttccactat cgctagtgct gatgcgcaag aaattgctac cgctctggct ggattcgatc
    34441 tggaaatgct ggatgcgcca gtttctggtg gtgcagtaaa agccgctaac ggtgaaatga
    34501 ctgtcatggc ctccggtagc aatattgcct ttgaacgact ggcacccgtg ctggaagccg
    34561 ttgccggaaa agtttatcgc ataggtgcag aaccgggact aggttcgacc gtaaaaatta
    34621 ttcaccagtt gttagcgggc gtacatattg ctgccggagc cgaagcgatg gcacttgcag
    34681 cccgtgcggg gatcccgctg gatgtgatgt atgacgtcgt gaccaatgcc gccggaaatt
    34741 cctggatgtt cgaaaaccgg atgcgtcatg tggtggatgg cgattacacc ccgcattcag
    34801 ccgtcgatat ttttgttaag gatcttggtc tggttgccga tacagccaaa gccctgcact
    34861 tcccgctgcc attggcctca acagcattga atatgttcac cagcgccagt aacgcgggtt
    34921 acgggaaaga agacgatagc gcagttatca agattttctc tggcatcact ctaccgggag
    34981 cgaaatcatg atcaagattg gcgttatcgc cgatgatttt accggcgcga cggatatcgc
    35041 cagttttctg gtggaaaacg gtctaccaac ggtacaaatt aacggtgttc caacaggtaa
    35101 aatgccggaa gcaatcgacg cactggtgat cagcctgaaa acgcgttcct gtccggttgt
    35161 tgaagccaca cagcaatcgc tggcggctct gagctggttg caacagcaag gttgcaaaca
    35221 gatctatttc aaatactgct ctactttcga cagtacggcg aaaggtaata tcggcccggt
    35281 taccgatgca ttaatggatg ctctcgacac gccgtttacg gtcttctctc cggccctgcc
    35341 ggtcaacgga cgtacggttt atcaggggta tttgttcgta atgaatcaac tgctggccga
    35401 atctgggatg cgccatcacc cggtaaatcc catgaccgac agctatcttc cccgtctggt
    35461 tgaagcgcaa tccacagggc gctgcggcgt cgtttcggca catgttttcg aacaaggtgt
    35521 ggatgccgtt cgtcaagagc tggctcgctt acagcaagag ggctaccgct acgcggtgct
    35581 tgatgcgctg accgaacacc atctggaaat tcagggagaa gccttgcgcg ataccccact
    35641 ggtaacgggc ggttctggtc tggcgattgg ccttgcccgg cagtgggcgc aagaaaacgg
    35701 taaccaggct cgcaaagcag ggcatccgct cgctgggcgc ggcgtagtgc tctccggttc
    35761 atgctctcaa atgaccaacc gccaggtagc acattaccgt caaattgcac cagcccgtga
    35821 agttgatgtg gcacgctgcc tctcaattga aactctggcc gcttatgcac acgaactggc
    35881 agagtgggtt ctgggccagg aaagtgtact tgctccactg gtttttgcca ccgccagcac
    35941 tgacgcattg gcagcaattc aacagcaata cggtgcacat aaagccagtc aggcagtaga
    36001 aacactgttt tctcaactag cagcgcggtt agcagcggaa ggcgtgacac gctttattgt
    36061 cgcaggcggt gagacctccg gcgtagtcac acagagcctg ggaataaaag ggtttcatat
    36121 tggcccaacc atttctcccg gcgtgccgtg ggtaaacgca ctggataaac ctgtctcact
    36181 cgcccttaaa tctggcaact tcggtgatga agcctttttt tcacgagccc aaagagagtt
    36241 tttatcatga gcgatttcgc aaaagtagag cagtctttgc gagaggagat gacgcggatt
    36301 gccagttcat tctttcagcg cggctatgca accggttcgg ctggcaattt gtcgctgctt
    36361 ttacctgacg ggaatttact ggcgacaccg acaggttcat gcctgggcaa tctcgatccg
    36421 cagcggcttt ccaaagtcgc cgcggatggc gaatggttaa gtggtgacaa accctcgaaa
    36481 gaggtgctct ttcatctggc gctgtatcgc aacaatccgc gctgtaaagc ggtggtgcat
    36541 ttgcacagca catggtcgac ggcgctttcc tgcctgcaag ggctggacag cagcaacgtt
    36601 attcgtccgt tcacaccata cgtggtgatg cggatgggaa atgtcccgct ggtgccttat
    36661 taccgaccgg gcgataaacg catcgcacag gatctggcgg aactggcagc agacaatcag
    36721 gcttttttac tggcaaatca tggcccagtg gtttgcggtg aaagcctgca agaagccgcc
    36781 aacaatatgg aagagctgga ggaaacggca aagctgattt ttattctcgg tgaccgcccg
    36841 atccgttatc tgaccgcagg tgaaattgcg gaattaagga gttaatgcaa tgcctcgttt
    36901 tgcagctaat ttatccatga tgttcaccga agtgcctttt attgaacgct tcgccgcagc
    36961 gcgaaaagcc gggttcgatg ctgtggaatt tctgtttccc tatgactact ccaccctgca
    37021 aatccaaaag caactggagc aaaaccatct gacactggcg ctgttcaata ccgctcccgg
    37081 agatattaat gcgggggaat ggggattatc cgcccttccc ggacgtgagc acgaagcaca
    37141 cgcagacatc gacctggcac tggaatatgc attggcgctc aattgtgaac aagtccatgt
    37201 gatggcaggc gtcgtgcccg ctggcgaaga tgctgaacgg tacagggcag tatttatcga
    37261 taatctccgc tacgctgccg accgctttgc gccacacggc aagcgaatat tagttgaagc
    37321 actcagcccc ggcgttaagc ctcattatct cttctccagc cagtatcagg cactggctat
    37381 tgttgaagaa gttgcgcgag ataacgtgtt tattcaacta gatacttttc atgcgcaaaa
    37441 agtagatggc aacctgacgc atttaatccg cgactacgcc gggaaatatg cgcacgtaca
    37501 aattgccgga ctacctgatc gacatgaacc ggacgatgga gaaatcaact atccgtggct
    37561 gttccgcctg ttcgatgagg tgggatatca gggctggatc ggttgtgaat ataaacctcg
    37621 tggcctcacc gaagaagggc ttggctggtt tgacgcctgg cgctaattcg ttaaatccct
    37681 aattacaacg tacccataca tccccccatc tacggatggg gtaggttaac tataactttc
    37741 agacagggtt tcccatgtcc acaattacat tgttatgcat tgcgttaact ggcgtaatca
    37801 tgctgttgct gctggtcatc aaggcaaagg tacaaccatt cgttgctctg ctcctcgtca
    37861 gcctgttagt cgcacttgcg gcgggtatac cggcgggcga agtgggtaaa gtgatgatcg
    37921 ccgggatggg cggtgtgctt ggttccgtca ctattattat tggtctgggc gctatgctgg
    37981 ggcgtatgat cgaacactct ggtggtgcag agtcactggc taattatttc agtcgcaagt
    38041 taggtgacaa acgaactatc gctgcgctga ctctggcagc gttcttcctc ggtattcccg
    38101 tcttctttga tgtcggcttt attattcttg cgccaatcat ttacggtttt gccaaggttg
    38161 ccaaaatatc gccactcaaa tttggcctgc ctgtcgctgg aatcatgcta actgttcatg
    38221 tggcggtacc gccgcatcca ggccctgtcg ccgcagcggg gttactccac gcagacatcg
    38281 gctggctaac catcatcggt attgcgattt ctattcccgt agggattgtt ggctactttg
    38341 cagcgaaaat aatcaataag tgccaatatg cgatgtcggt ggaagtactg gaacagatgc
    38401 aactggctcc ggccagtgag gaaggcgcga caaaattaag cgataaaata aatccaccgg
    38461 gcgtcgcgct ggtcacctcg ctaattgtta ttcctatcgc gattatcatg gcgggtacgg
    38521 tttccgcaac actgatgccg ccttcgcatc ccctgcttgg tacgctacag ctgatcggct
    38581 caccaatggt agctctaatg attgcgctgg tgctggcatt ctggttattg gctttacgtc
    38641 gcggctggag cttacaacac accagcgaca ttatgggctc agcccttccc accgcggcgg
    38701 tagtgatttt ggttactggt gctggagggg tatttggcaa agtgctggtg gaatcgggcg
    38761 tcggcaaagc ccttgccaac atgctacaaa tgattgacct gcctctgtta ccagccgcat
    38821 ttattatttc actggcgctg cgtgcatcgc aggggtcagc aaccgtagca atcctgacaa
    38881 ccggcgggtt actctcagaa gcggtgatgg gattgaatcc gattcagtgc gtattggtga
    38941 cgctggcagc ctgctttggt gggcttggcg cgtcacatat taatgactca gggttctgga
    39001 ttgtgaccaa atatctgggg ttatcggtag cagacggtct gaaaacctgg acagtgttaa
    39061 cgaccattct cggttttacc ggattcttaa ttacctggtg cgtatgggcg gtaatttgac
    39121 cttaataaaa aggtccgatg ggcatcggac cttttattta gcgcttacct tccgccagca
    39181 gcggcgggat gctcggcacc atcggcgcgt catcaatatc tttctgtgtc atgcggaacg
    39241 cttcgggata atgttcgcgg ctggtgcggc gtagcggttc ggtatcgcgc caggtataga
    39301 ggcaatgctg gcactggtag accgtccaga catcttttac cggcgatttc gccatcactt
    39361 caatctgttc atcagcacaa cgtggacaaa tcatcttgtt ctccttattt acgtgcggcc
    39421 agcatagcgg tcagtttttc agcccaggct ttggtttccg gtaaatccac caccggctgg
    39481 ctgtagtgac cacggttgtc cggggcgaca ggcgtggtgg cgtcgataat cagcttgtcg
    39541 gtgataccgg ccgggcttga gccggggtcg agttccagca cggacatatt cggcaactgc
    39601 accaaatccc ctgccgggtt cactttcgaa gagagcgccc acatcacctg cggcaggttg
    39661 aacggatcaa cgtcttcatc gaccataatc accatcttca cgtagcccag accgtgtggc
    39721 gtggtcatcg cacgcaggcc caccgcgcgg gcaaagccac cgtagcgttt tttggtggag
    39781 ataatcgcca gcaggccgtg ggtgtacatg gcgttcaccg cctgcacttc cgggaactcg
    39841 gctttcagtt gctgatacag cggcacgcag gtggctggcc ccatcaggta gtcgatttct
    39901 gtccacggca tgccgaggta cagcgattcg aaaatcggcc tggtgcggta agagacttta
    39961 tcgatacgca ccacggtcat gttacgcccg ccggagtagt gcccggtaaa ctcaccgaac
    40021 ggcccttcga tttcgcgttt acgactttcg atgacccctt cgaggatcac ttctgaaccc
    40081 cacggcacat caaaaccagt cagcggggcg gtggcgatcg ggtacgggct ttcacgcagc
    40141 gcgcctgcca tttcgtactc ggactgatca tatttcagcg gcgtggcccc catcagcgtg
    40201 atgatcggat cgttacccaa ggtgatggca atcggcagat cttcaccgcg ttcttccgct
    40261 ttatgcagat gcagggcgat atcgtgcatc ggcaccggtt gcaggccgag cttacgtttg
    40321 cccttcactt ccatacgata gataccgacg ttctgcttgc cgaagttatc cgggtcgagc
    40381 ggatcgcggg aaaccacgca cgctttgtcg agatagaaac cgccatcgcc atcgtttaaa
    40441 cgaaacagcg gcaggatatc gaacaggttg atctcgtcgc catcgacggt gttctgcgcc
    40501 caggctggat tggcgcggcg ctccggggca atcgggaagt tatcccagcg gcggataaac
    40561 tcatcaatct gctttttgac cggggtgttt ggcggcaggc cgagggaaat cgcgtggttc
    40621 tgccaggaac cgatggtatt catcgccaca cgggcatcgg taaagccgcg aatattgtca
    40681 aaccacagcg cgggcgcgcc gtcgccgata cgcccggtgg cgttagctgc tgctgccaga
    40741 tccggttccg cgttcacctc ttcgctgatt ttcagtaact gtccgtggtc atcaagcgcc
    40801 tgtaaaaagc tgcgtaaatc atcaaatgcc attattcatt ctcctgagaa aaattccgga
    40861 cctgcggcaa tccttgccag cgcctggcgt ggggatgttc gaggccaaat tgatccagca
    40921 cgcgggctac cacgtggtgg acaatgtcat ctaccgtttc gggatggtta taaaaggcag
    40981 gcatcggcgg caccatcgcc acgcccatgc gtgaaagtgc gagcatattt tcgagatgga
    41041 tggtgctaag cggcatttca cgcggcacca gcactagttt gcggccttct ttgagcacga
    41101 cgtccgccgc gcgccctacc aggccatcgg cgtaaccgac acggataccg gcgagcgttt
    41161 tcatactgca cggaataacg atcatgccgt cggtacgaaa ggaacctgag gagatggtcg
    41221 ccgcctgatc cgccgggtta tgactgaagt ctgcgagggc agcaacatcg cgagcgctgt
    41281 aaggcgtttc cagttcaatg gtggttttcg cccacttcga catcaccaga tgagtctcga
    41341 cattcggcat ctcccgcagc gcttgcagta atgccacccc aagaggcgca ccggtagccc
    41401 ctgtcatccc gacgatcagt ctcattgttt attctcttaa ttgttcgtat acgaacgtta
    41461 ttattaaaat acgcctctgt tttcatcgcg gcaagcactg aaaagtaaca acatcttctt
    41521 cgtcactgaa aaaccgctat gatagcagca gatagtttgc ccggagttta catggcgtta
    41581 cgaaataaag cgttccatca gttacgacag ctttttcagc agcacacggc tcgctggcag
    41641 cacgagttac ctgacctcac caaaccacag tacgcggtga tgcgcgccat tgctgataag
    41701 cctggtatcg aacaggtggc actgatagaa gcagcagtca gcaccaaagc aacgctggca
    41761 gaaatgttgg caagaatgga aaatcgtggc ttagtcagac gtgaacatga cgccgctgac
    41821 aagcggcgtc gctttgtctg gctgactgcc gaaggagaaa aggtacttgc tgcggcgatc
    41881 cccattggtg acagtgtgga tgaggaattt ttggggcgtt tgagtgcaga agagaaggag
    41941 ctgtttatgc agttggtgcg caagatgatg agcaaatagg atgctgattg ccgggtagga
    42001 cgctgacgag tcttatccag gcgacaaaac acaccgactt cccaaacaaa agaaaaaggc
    42061 cagcctcgct tgagactggc ctttctgaca gatgcttact tactcgcgga acagcgcttc
    42121 gatattcagc ccctgcgttt gcaggatttc gcgcaaacgg cgcaggcctt caacctgaat
    42181 ctggcgaaca cgttcacggg tgaggccaat ttcacgacct acatcttcca gtgttgccgc
    42241 ttcgtacccc agcaaaccga atcgacgtgc cagtacttca cgctgtttgg cgttcagctc
    42301 gaacagccat ttgacgatgc tctgcttcat atcgtcatct tgcgtggtat cttccggacc
    42361 gttctctttt tcatcggcca ggatgtccag caacgctttt tcggaatcac cacccagcgg
    42421 ggtgtctacc gaggtaatgc gctcgttaag acgaagcata cggctgacgt catcaactgg
    42481 cttatccagt tgctctgcga tctcttccgc acttggttcg tggtccagtt tatgggacaa
    42541 ctcgcgtgcg gttcgcaggt aaacgttcag ctcctttacg atgtgaatcg gcaaacgaat
    42601 agtacgggtt tggttcataa tcgcccgttc gatcgtctgg cgaatccacc aggttgcgta
    42661 tgttgagaag cggaaaccac gttccgggtc aaacttctct accgcgcgga tcagccccag
    42721 gttgccctct tcgatcaggt ccagcaacgc cagaccacga ttgccataac ggcgggcaat
    42781 ttttaccacc agacgcaagt tactctcgat catccggcgg cgggaggcaa catctccacg
    42841 cagtgcgcga cgcgcaaaat aaacttcttc ttcggccgtt aacagtggtg aataaccaat
    42901 ctcaccaagg taaagctgag tcgcgtccaa cacacgctgt gtggctccct gcgataacag
    42961 ttcctcttcg gccaaatcgt tatcactggg ttcctcttct actaaggcct tttcgtcaaa
    43021 aacctcaact ccgttctcat caaattccgc atcttcattt aaatcatgaa ctttcagcgt
    43081 attctgactc ataaggtggc tcctacccgt gatcccttga cggaacattc aagcaaaagc
    43141 ctggttccgc cgatttatcg ctgcggcaaa taacgcagcg ggtttacgga tttccccttg
    43201 taacgaattt caaaatgcaa gcgtgttgaa ctggttccgg tgctacccat ggttgctatt
    43261 ttttgccccg ccttcacttc ttgttgttcc cggaccagca ttgtgtcgtt atgggcgtag
    43321 gcactcaggt aatcatcatt atgtttgatg ataatcagat taccgtagcc gcgcagcgcg
    43381 ttaccggcat aaacaacgcg gccatctgcg gtcgcgataa ttgcctgtcc tttgctgcct
    43441 gcgatatcaa tccccttgtt gcccccctca gaagcgccaa aggtttcgat cactttgccc
    43501 tcagtcggcc agcgccaggt ggagataggc gtactggttg atgtactgct gacagtcggc
    43561 tcggttgtgc ttgctgttgg caccgttaca ggcgctgtga ccgtggtcgc agttggcttg
    43621 ttgttcggca acattttgtt agcactctgt tcacccgaag actcagaata cgtaattgtc
    43681 ggttgcgacg caacagcaac ggtggaattt tgtgcaggct tgatcacaac tccttgctct
    43741 gctgcgtcgg cctgggtaat ggcatttccg ccagtgattg gcgtaccgga agcattaccc
    43801 acctgcaagg tctgaccaac gttcagcgcg tatggtgcct gaatattgtt gcgctgagca
    43861 aggtcacgga aatcgttgcc agtaatccag gcgatataga aaagtgtgtc gccttttttc
    43921 acggtatagg tactgccgct ataactgcct ttcggaatgt tcccatactg acggttatag
    43981 acgatgcgtc cgttttccat ctgtaccggc tgctgagcta ctggctgcac cggctggatt
    44041 tgcggttgtt gagtggcctg aatttgtggc tgctgtaccg gctgaatttg cggttgctga
    44101 gctgtagacg tcgttcccat tttcggcggc ggcgtaatca acataccaga attggtattt
    44161 gcaggcgcat tgccattaac ggagctgacc ggtgccggtg gatttgaagt gtcagaacag
    44221 cctgccagcc atagcgaaac cagtgacaaa gccgcaatgc ggcgaacggt gaattttggg
    44281 cttcccgcgc tcatttatcc cccaggaaaa attggttaat aaccagtgac ataattaccg
    44341 tgcaaggcac catactgaac actggaaaag atgttcacga tacgctgacc tgcggcaaaa
    44401 taaccaggaa aaatccaggt atttcctcac gttttaagcc agctcaccct tcactaaagg
    44461 gacaaagcgc acggcctcca cggtatcgat aataaattcg cctccccgac gacgcacccg
    44521 tttcaaatac tggtgctcct cccctacggg taagacgaga atcccgcctt cgtccagctg
    44581 cgtcattagc gcagttggaa tttccggcgg tgccgccgta acaatgatag cgtcaaacgg
    44641 cgcacgtgcc tgccaacctt gccatccatc gccatgacgg gttgaaacat tatgtaaatc
    44701 aagatttttc aggcggcgac gcgcctgcca ctgcaagcct ttaatccgtt cgaccgagca
    44761 aacatgctgg acaagatgcg ccaggattgc cgtttgatat cccgaaccgg tgccaatttc
    44821 cagcacccgc gactgcggcg tcagttcgag taattcggtc attcgcgcca ccatatacgg
    44881 ctgcgaaatt gtctgcccct gacctatcgg caaggcgata ttgtcccagg ctttttgttc
    44941 aaacgcttca tcaacgaatt tttcacgcgg cacggcggca agtgcattca gcacctgctc
    45001 atcctgaata ccttgcgcac gtaattgatc cagaagtgct tgtacgcgtc tgcttaccat
    45061 tgcgtgccaa ctcccacgct gtttaaccag tctgaaacca catcttgcgc gctatgcgca
    45121 gttaaatcca catgcagcgg cgtgatggag acatagccct catctaccgc agcaaaatcg
    45181 gtccccggac cggcatcaca tttaccgccc ggcgggccaa tccaatacag cgtattgccg
    45241 cgcggatctt gctgcgggat cacctgatct gccggatgtc gtgtaccgca gcgcgtcacg
    45301 cgaatacctt tgatttgatc caagggtaaa tccggaacgt taatattaag aatacgtccg
    45361 gtgcgcagcg gctctttaca cagtgcgcgc aaaattgaac aggttaccgc cgcggcagtg
    45421 tcgtaatgtt tatgcccgtc aagcgagacg gcaagcgccg gaaaacctaa atgacggcct
    45481 tccatcgcgg cggctaccgt accggaataa ataacatcat cccccagatt cggcccggcg
    45541 ttaattccgg acacaacaat gtccgggcgc ggacgcatca gagcattcac gccaagatag
    45601 acgcaatcgg tcggggttcc catttgcaca gcaatatcac cattttcaaa ggtaaacgtg
    45661 cgcagggagg attccagtgt tagagaattt gaagcgccgc tgcggttacg atcgggggcg
    45721 accacctgaa cgtcagcaaa ctcacgcaag gctttcgcca gcgtttgtat accgggtgca
    45781 tgtaccccgt catcattact cagcaatatg cgcataatca cctgttgtgt tgataagttc
    45841 cctgacaacg ctggttgcaa aactacccgc cggaagccag aaacggattt ctacggtgac
    45901 gtcatcccac cagttccagc ttaattgttg cggatacagc agcatcgctc tgcgcgcggc
    45961 ttcaactttt tcgcgcacca gtaaggcttg taattcagtt tctgcggcga cagctgcttg
    46021 ttcgaatgcc agcgcttcac gctgagttcc ccattcgcca ctgcccggca atgcggcggt
    46081 tatcattagc tctttatcgt tgacgcgacg ctgtaattcc gccagttctt cggtggttgc
    46141 gacaaaccag ctaccacgtc cggctaattg tagcgcatcg ccgtcaacaa cttgattaac
    46201 gtctgctttt ttgaggcgct cagcaacaat ctgattaaac aacgcactgc gggctgccga
    46261 caaccaaaaa ctccgtttat tgcgatcgcg caccggagta ttggtttgcg cccagcgcag
    46321 cgcgccctgc aagttgctac cgccaatccc aaaacgttgg gcaccaaaat agttaggcac
    46381 gccttttacg caaatatcga tcagacgttg ttcaacgtca tcgcgattgc tcacttcgcg
    46441 cagaaccagg gtaaaggcgt tacctttcag cgcgcctaaa cgcagcttgc gcttgtgccg
    46501 cgcatactcc agcacctggc agccttccag ttgaaaggcg ctcagatcgg gcatttcctt
    46561 gcccggcacg cgagcacata accactgttc cgttacagca tgtttgtctt tttgcccagc
    46621 gaagctgact tcacgggcat gaattttaag gaatttcgcc agtgcatccg ccacaaaacg
    46681 ggtattgcag ccgtttttga ggattctaac cagaatatgc tcaccttcac catcaggctc
    46741 aaagcccaaa tcttccacca ccacaaagtc ttccggattg gctttcagca gcccagtgcc
    46801 ttgcggttta ccgtggaggt aagtgagatt atcaaactca atcattttgt tgccttaatg
    46861 agtagcgcca ccgcttcaca ggcaatccct tccccacgtc cggtaaatcc aagtttttcc
    46921 gtagtagtgg ctttcacgtt aacatcatcc atatggcagc cgagatcttc ggcaataaac
    46981 acgcgcattt gtggaatgtg cggcaacatc ttcggtgcct gagcgatgat agtgacatcg
    47041 acgttgccca gggtataacc cttcgcctga atacgacgcc aggcttcgcg tagcagctcg
    47101 cggctgtcgg cacctttaaa tgccggatcg gtatccggga acagtttgcc gatatccccc
    47161 agcgccgccg cgccaagcaa tgcatcggtc aacgcatgga gcgccacgtc gccatcagaa
    47221 tgcgccagca atcctttttc gtaaggaatg cgtacgccac caatgataag tgggccttca
    47281 ccgccaaagg cgtgtacgtc aaaaccgtgt ccaattcgca ttatgtattc tcctgatgga
    47341 tggttcgggt gaggtaaaac tcggccagtg ccaaatcttc cggtcgcgtg actttaatgt
    47401 tatccgcgcg gccttcgacc aactgcggat ggaatccgca atattccagc gccgaggctt
    47461 cgtcggtaat ggtagcgcct tcatttagag cgcgcgtcag gcagtcatgt aacagctcgc
    47521 gagggaaaaa ttgcggcgtc agcgcgtgcc ataagccgtt gcgatcaacg gtatgagcaa
    47581 tggcattttt gcccggttcg gcacgtttca tcgtatcgcg cactggtgcg gcgaggatcc
    47641 cccccgtgcg gctggtttcg ctcaacgcca acaatcgcgc gaggtcatcc tgatgcagac
    47701 aaggacgagc ggcgtcatgc accaataccc actgcgcgtc gccagcggct ttcagacctg
    47761 ccagcaccga atcggcacgc tcatcaccgc catctacaac ggtgatttgc ggatgattcg
    47821 ccagaggaag ttgtgcaaaa cggctatcgc caggacttat ggcaatgacg acacgtttca
    47881 cccggggatg cgccagcagc gcatgcaccg agtgttcaag aatggtttga ttaccgattg
    47941 agagatattg cttaggacat tccgtttgca ttcgacggcc aaatccggcc gccggaacca
    48001 cggcgcaaac atccaaatga gtggttgcca tgttaattcc cgggctgatt tatcgattgt
    48061 tttgccccgc agactgtgcg cgcttcgacg cgtcaggcac cagacgataa aaagtttcgc
    48121 ccggcctggt catgctgagt tcattacgcg cacgctcttc gagcgcctcc tggccgccat
    48181 tgagatcgtc aatttcggca aaaagttgat cgtttcgcgc tttaagtttc gcgtttgtag
    48241 cttgctgtgc cgccacatca tcattgacgc gggtatagtc atgtataccg ttcttaccga
    48301 accacagcga atactgtagc cagaccagaa tagccagcaa cagcagcgtt agtttaccca
    48361 tcctgccccc tgaaaaacgg catcatcatc ccatgcatcc gaagacgact ctacatcctc
    48421 tgttggggat accgcgacaa cgcgggcaaa tgtaccacat ttgtccattg ttacgtatac
    48481 ccagggcgtg cagaacataa tctcattatt agttacggtt tgaattatga acagaggaga
    48541 caagaaagta caaattagcc cagtagccac ataaacagtg cgccaaacat aatgcctact
    48601 gtcaccaggg tgaaaacaat actgtagcgt agctttccgt ccatcaatga atgcagcgca
    48661 atccccacca ctaccgcgac aggcatcagt gccagaaaga aaggccaggt gtagataaag
    48721 aaaaacagcg tgttagagcc ataaatcaac atcggcatcg ccagcgcaaa taaccaggag
    48781 ataaaaccga ccacagcacc aggcagtgac catgtggttt cttcatcctc agtaaggctg
    48841 tcgttatttg ttagtgtaat gttatggcta ttacgcatat ttgatcctgt tactttgacg
    48901 aaccgggcat ggaaacccgg tggtgtctca ggatctgata atatcgttct gtctcaacag
    48961 atctaataat tgctgtacca aatttgttac taattgttca ccattgagat gaatttctgc
    49021 cgattcaggc gcttcgtaaa cggaatctat tcccgtaaag ttgcgcagtt caccggcacg
    49081 cgctttctta tataagcctt ttggatcgcg ggcttcgcaa atcgccagcg gcgtatcgac
    49141 aaacacttcg ataaagcgcc cttctcctac gcgttcacga accatctggc gttcggcgcg
    49201 gtgtggcgag atgaatgcgg tcagcaccac cagtccggct tcaaccatca aattcgccac
    49261 ttcaccgacg cgacggatat tctctttacg atcggcatcg ctaaaaccga gatcgctgca
    49321 taatccgtgg cgaacattgt cgccatccag cagatacgta ctgacgccga gtttatgtaa
    49381 cgcctcctcc agcgccccgg cgaccgttga tttaccggac ccggagaggc cggtaaacca
    49441 cagcactaca ccacgatgac cgtggtgtag ctcgcgttgt tgcacagtga ccggatggct
    49501 atgccagacg acgttttcgt catgcagcgc cattatttat cccccagcaa atcgcgtgcg
    49561 ccccagtgcg gaaagtggcg acgaaccagg gcattcaatt ccagttcgaa tgcactgaat
    49621 tcagatggcg cagcagttgc ctggctaact ggctcgtgca ccataccggc acccacggtc
    49681 acattgctca ggcgatcgat aaaaatcagc ccaccggtaa ccggattttg ctgataacga
    49741 tctaacacca gtggctcgtc aaaagtgaga tccaccaggc cgatgccgtt cagcggcagg
    49801 ttttcaactt cgcgttgggt aaggttatta atatcaacct gataacgaat gccatcaaca
    49861 cgagcacgtg tcttcttacc ggcaattttg atgtcgtaac tctggcctgg ggaaagcggc
    49921 tgttccgcca tccataccac atccaccgac gcgctctgca cagccggtaa cgcttcgtct
    49981 gccgccagca gcagatcgcc acggctgatg tcgatctcat ccgtcagcac tagggtgatc
    50041 gcttctccgg caaaggcttc ttcgcgatcg ccatcaaaag tcacgatccg cgcgacgttt
    50101 gattccacac cagagggcag cactttgaca cgttgcccga cttccactcg accggatgcc
    50161 agcgttccgg cgtagccacg aaaatcgaga ttcgggcgat taacgtactg caccgggaag
    50221 cgcattggct gggcatccac cactcgctgg atctccacgg tttccagcac ttcgagcagt
    50281 gtcggaccgc tgtaccacgg catactttca ctttgcgaag ccacgttgtc accttccagt
    50341 gcagagagcg gcacaaagcg gatatccaga ttacccggca gctgcccggc aaaggtcaga
    50401 taatcttcgc gaatacgggt gaacgtctct tcactgtaat ccaccagatc cattttgttg
    50461 atcgccacga ccagatgttt gatccccaac agtgtggaga taaaactgtg acgacgggtt
    50521 tgatcgagca cgcctttacg ggcatcgatc agtaagatcg ccagttcaca tgtcgatgcg
    50581 ccagtcgcca tattgcgagt gtactgctcg tgccctgggg tatcggcgat aataaattta
    50641 cgcttctcgg tagagaaata acggtaagcc acgtcaatgg tgatgccctg ttcgcgctca
    50701 gcttgcaggc cgtccaccag cagtgccaga tccagttttt cgccctgggt gccgtgacgt
    50761 ttactgtcgt tatgcagcga tgagagctga tcttcgtaga tttggcgggt atcgtgcagc
    50821 aggcgaccaa tcagggtact tttgccgtca tcgacgctac cacaggtcag aaaacgcagc
    50881 aggcttttat gttgttgtgc aatcatccag gcttcgacgc cgccttcatt ggcgatttgt
    50941 tgtgcaagtg cggtgttcat cttaaaaata cccctgacgt tttttcaact ccatggatcc
    51001 cgcctggtcg cggtcaatca cgcgaccctg acgttcactg gtggtggaaa ccagcatctc
    51061 ttcgatgatt tccggcagtg tttgtgcatt tgactccacc gcaccggtca gcggccagca
    51121 gcccagcgta cggaaacgca ccatccgttt tttaatcact tctcccggtt gcaggtcgat
    51181 acggttgtca tcaatcatca tcaacatacc gtcgcgttcc agaaccggtc gttccgcagc
    51241 gagatacagc ggcacaatgt cgatattttc cagccagatg tattgccaga tatcctgctc
    51301 ggtccagtta gagagcggga agacgcgaat gctttcgcct ttgttaattt gcccgttgta
    51361 gttgtgccac agctccgggc gctgattttt cggatcccag cgatggaagc ggtcacggaa
    51421 agagtagatt cgctctttag cacgggattt ctcttcgtcg cggcgtgcac caccgaaggc
    51481 ggcatcaaaa ccgtatttat tcagcgcctg tttcaggcct tcagttttca taatatcggt
    51541 atgtttcgcg ctgccatgaa cgaatggatt aatccccatc gccacgcctt ccgggttttt
    51601 atgcaccagc agctcgcagc cgtaggcttt agccgtacga tcgcggaact catacatctc
    51661 gcggaatttc cagccggtat cgacatgcag caacgggaaa ggcagcgtac ctggataaaa
    51721 cgccttgcgc gccagatgca gcatgacgct ggaatcttta ccgatagagt agagcatcac
    51781 cggatttgag aattctgccg ccacctcgcg aataatgtgg atgctttccg cctccagttg
    51841 ccgcaggtga gtaagtcgtt tttgatccat aaccgttcct ttgcaatacc gctattttct
    51901 tgccatcaga tgtttcgact atagggagcg taagagaacg aatgaaatta ccaattagaa
    51961 tgagtagttc cttaacggaa taacgatttg gcaaagctaa tatcaaaaag tgcttaaggc
    52021 accggatttc gggcgtttag gaagatttga aattgtttta gcgcatcggc agtttcatac
    52081 tatggcggta aaaaaatttg catggtattt aaggactcac tatgttttcc gcattgcgcc
    52141 accgtaccgc tgccctggcg ctcggcgtat gctttattct ccccgtacac gcctcgtcag
    52201 ctaaacctgg cgattttgct aatactcagg cacgacatat tgctactttc tttccgggac
    52261 gcatgaccgg aactcctgca gaaatgttat ctgccgatta tattcgccaa cagtttcagc
    52321 aaatgggtta tcgcagtgat attcggacat ttaatagtcg gtatatttat accgcccgcg
    52381 ataatcgcaa gagctggcat aacgtgacgg gaagtacggt gattgccgct catgaaggca
    52441 aagcgccgca gcagatcatc attatggcgc atctggatac ttacgccccg ctgagcgatg
    52501 ctgacgccga tgccaatctc ggcggactga cgttacaagg aatggatgat aacgctgcag
    52561 gtttaggtgt catgctggaa ctggcagaac gcctgaaaaa tacgcctacc gagtatggta
    52621 ttcgatttgt ggcgaccagc ggcgaagagg aagggaaatt aggcgctgag aatttactca
    52681 agcggatgag tgacaccgaa aagaaaaata cgctgctggt gattaatctc gataacttaa
    52741 ttgttggcga taaattgtat ttcaacagcg gtgtaaaaac ccctgaagca gtaaggaaat
    52801 taacgcgcga cagggcgctg gcaattgcgc gtagtcatgg aattgccgca acgaccaatc
    52861 cgggtttgaa taaaaattat ccgaagggta ctggctgttg taatgatgcc gaagttttcg
    52921 acaaagccgg tattgccgta ctttcagtag aagcaacaaa ttggaatctt gggaataagg
    52981 atggttatca gcaacgcgca aaaacagctg cattccctgc gggaaatagc tggcatgacg
    53041 taagactgga taatcaacaa catattgata aggcacttcc tggaagaata gaacgtcgct
    53101 gccgtgatgt tatgcggata atgctaccgc tggtgaagga gctggcgaag gcgtcttgat
    53161 gggtttgaaa atgagagctg ggagttctac cgcaggggcg gggaactcca agtgatgtcc
    53221 atcatcgcat ccagtgcgtc cggtttatcc ccgctgacgc ggggaacacg tcaataggcg
    53281 gcgtcccgta gccgtcccct tcggtttatc cccgctggcg cggggaacac gttgaggtcg
    53341 ataagtacga acggtttccc tgcggtttat ccccgctggc gcggggaaca cgatcgcctg
    53401 ctccagcgtc actccctgcc cctcgattta tccccgctgg cgcggggaac acttctgata
    53461 cgtactgagg ccggttgtga tacgcgattt atccccgctg gcgcggggaa cactaacccc
    53521 caatgaagtt aataaagtct atggccgatt tatccccgct ggcgcgggga acacatgaga
    53581 ttaaaatact cagtactctt actcgccgat ttatccccgc tggcgcgggg aacaccagcg
    53641 tggcgcgata aagcaatgtg tctggctcga tttatccccg ctggcgcggg gaacaccaga
    53701 ggcaaccgac gcggattcag taggaaagcg atttatcccc gctggcgcgg ggaacaccga
    53761 tagacacttt gcggtcattg tctattcggc gatttatccc cgctggcgcg gggaacacac
    53821 taagcataca taactgtttt taaacaaatt tattccgcat caacaatcta ccaactaaat
    53881 taaaacattt ccttattttt aaagaacaga taacttattg attatcaaca ggaagaaaag
    53941 aaaccaaacg caccccatcc aaatccaccg gaatacgtct attttcaccc caggtctgaa
    54001 attcaaaacc cgactcggta ttggtcgccc aggccattac cacatttccg caacctgcca
    54061 gttgagagat ttgctgccag atcatctccc gaatacgttt ggacgtatct ccgacataaa
    54121 caccggcacg cacttccagg agccagatcg cgatccgtcc acgtaagcgc ggagggacat
    54181 tttctgtaac aacgacgacc atactcatcc gccgcgtccc cggtgaccgc tatcgcccag
    54241 cgtttcaggt tcagggatgg caggcggtaa catatccggc gcgggttgtg gtggttcaat
    54301 ttcacctgcg gcaagcactt cctcgattaa cggaattaat ttgcccgtta acttactgct
    54361 acggaaaata tcacgacagg ccaacctgac ttctttatct ggttctgcgg gttgcctcgc
    54421 tgctatttca aatgcctttg gcacaaccga atcaaattta atgatatcgg ctatgtcata
    54481 aacaaatgaa agcggtttgc cactatggat aaatccaata gcgggcgcat atcccgcggc
    54541 taatactgcc gcttcagaaa taccgtacag acatgatgtg gcagcactga tgcagcgatt
    54601 cacaacatcg cctttttccc agtctttagg atcgtatttg cgaccattcc atttcacacc
    54661 atattgtttc gccagtaatg catacgtctg gcgaacgcgg gatccctcaa ttccacgtag
    54721 ctgctccact gaacggcgag ctggcggtgg ctcacgaaaa cgtaattcat acattttgcg
    54781 caccaccttc agacgtagat cttccgttaa agccagcttt gcctggtaga gtaacttatc
    54841 agcacgcgcc ccaccgggtt gtccggaaga gtagacgcga acacccgctt caccgaccca
    54901 gaccagtagc gtccccaccg tggcggccag atgcaccgcc gcgtgggaaa ctctcgttcc
    54961 cggttcgagc ataatgcagg cgaccgatcc caccggaatg tgcgtgcgga tcccggtttt
    55021 gtcgatcagc acgaaagcgc cgtccagtac gtcgatttga ccgtactgga ggaagatcat
    55081 agaggtgcga tcttttaacg ggatcggact cagtggtaca aacgtcacgc ctctgctccg
    55141 ggtttgatca gcatcagacc acagccgaac gcgcggcttt tgccataccc ctggctcaga
    55201 cgttgcagaa ataacccagg atcggtgacc gtaagcatcc ccgtataatc cacgctactg
    55261 aactggatca gttgccggga gttttcccgc cgcagttgct gttgtcggta ggcatcgacc
    55321 gaggtatcca gtagtgtaaa accgcttctt tccccctgcg ctgccagcca gtccagcgcc
    55381 gcctgttgtt gatgcaacca gacatcactt ccttccacct gccccctcac ctgccgtttc
    55441 gcctccatca gcaagtcgtg gcgtttgccc gctttacaaa ttgttggatt cgcccgcagg
    55501 ttgaaacaaa gggattgtcc ggtacgcaac tctggcgcaa atggtcggca ttcgatggtg
    55561 aaaatatcac tttccgccgg gcgttcctgc gacaggacaa aaaagcgaaa cgccccctgg
    55621 agttcttccc gacgataaag aaattgcctt tctttgccgc caggaaaaag atcccacagc
    55681 cactgatgca ttacatattc accgcgaccc accaaatgca gcaactgcgc aggcaaaagc
    55741 tggccggtat gtaaggttat tcttgagagg tacatggttc ctccttgctg agccacggcc
    55801 cctgattgat ggtgcgttcc ccaaacagcc actgctgacg atttaaagga acatcccggc
    55861 gacgtaatat tttgctcgcg accaggccgt cgtgttcccc ttcccaccag cattcatcct
    55921 gaagtttcgg gagtgagact ttcagttcgc ggaaactatc ctgatactgt tggtatgcgt
    55981 tacgtaagac atcagacgcg ttgccttcga gcagtaacgg cgcaagtggt aacgccagcg
    56041 gatgactttt tcgccccaga taaagcggaa aaaccggatg acgtaaaccg tcctgcaact
    56101 gttcaagcct gtaagacgca tcgggggttg ttgccaccgc caccatccac caggcatcgg
    56161 tgtagtagtc gcgccgggag ataatcgcgc tcagaagatc aggggcgctc aactcttcgc
    56221 gacggctgaa ataacgcgct ttacgcacct cttttggcat ctggaccgtg tgataatccc
    56281 gtgcccagcg cgggttacgg ctggcgcaaa ccaccagtga atagtggcgg ttaaacgcgt
    56341 ttaatcgttc ggtatcatca cgccgaatcc ctaccccggc agccagcagc cccagcaatg
    56401 ctgagcgcga aggcagttca tgggtatgac gcacttcgcc gggggcatcg acgccccagg
    56461 atgccattgg cccatgaagc tgaaaaatca aatattggct cattagccgc cccttacgcg
    56521 cagataaagt ccagcacgtc cttcatgctt ccctgtttgt tcatcacgtc aaagcttacg
    56581 cattcggtct tctgttcata gaccgtattc atattttcgc gaagcgttgt aatacgctgc
    56641 accgccacat ctaactgccg ggtgccatta atgggttcat agaaagccgc cgccagagag
    56701 cgtggttgtt cagtgccttt ttctgccagc gcccaggagg cgtaggcacg gctggcaaag
    56761 ctgttctgtt tgccggttgg ggagacttta agtgcggctt ccgtaaaggc gcgcaaggtc
    56821 tgattagcta acgcttcgtc accgccgagg ttttcgacca gcagatcttt atcgatgcag
    56881 atataggtgt agaacagcgc agaaccgaat ccggtttcac caagatgccc ggcaccggca
    56941 tcttcagaag cctggcgcaa atcatcaacg gcggtgaaaa aatcatcttc gacaatcgtt
    57001 tcactgacac caaatgcatg cgcgacctgg caggcggctt caacattaaa ttcgggttta
    57061 ttcgccagca tacgaccaaa catagcgata tctaccgcca tgcgatcttt acgtaacaaa
    57121 gcgagatctt cctcttttgg cgcgcgcttt tcttcggcca gttgatgggc cagcgctttt
    57181 acggcgtcaa attctgccgg gctgatatgg actagctgtt cagtttcggc gttagtgagc
    57241 ggatcttttg gttttttgtc gtttttagct ttcccaagat aatccgcaat ttttgccgcc
    57301 cattcgatgg cttttttctc ttcgatgcct ttctcaatca ggatagttgc cgcctcacgc
    57361 gcaatacgcc cactgcgaat accaatatgg cccgccagtg cctgttcaaa aagtgcagaa
    57421 gtgcgccacg cacgtttcag actttgtgag gaaacgcgca gacgcgttgc tccacccagt
    57481 accacagttt ttggcgcacc ggtgtcatca cggttaaggt tggcagcagg gtaagcggtt
    57541 aacaaatgaa gctgaataaa tgtcgtcatg agatagtcct ttattaattt tgttcgttat
    57601 cggcgtcgcc agcctggtaa tattccagcg cccagcggat acggataaat tctgttggcc
    57661 gctgctggcg gcggtgatga ttgagcaggt cgtcgctctc ctggcaccag cggaaaatat
    57721 cttctgccaa agagataaga ttgacggaac catttaataa tttgaccgcg cgacgtaact
    57781 gacgcagtaa atcatcaggc gtttttactg ccgacaggcg agtaaaacgc ccctccgaca
    57841 ttaccgccgc cagctgcgcg gcaaaaggct gccgttcatc aatagcttta acattggcgc
    57901 taactgcggc gaccagcgcc agtgcggtaa aacgccactc ttgtgcttca attttccaca
    57961 aagtgtgggt ctgcatcagc agagaacgaa acccttctga cagacaaaca tcattaaccg
    58021 ttgtgctacg ccgcagactg gcacgattgc cgcgtttttc ctgcaactct tcgtgccatt
    58081 tacgcagcgt ggctttgtgc tcctctttta caatactcat tcagcagcct cctgcttttt
    58141 ctccctggcg gctttagcat tttgcttctc cgccgatgtt gtaaaatatt tcttgcgcgc
    58201 ggtcatgacg cgtttcaaat caacgggctc atatggattg gtgaagacac gctcgtcaaa
    58261 atcctgacgc gcgaataacc aaatttcctt ttgccatttg ctgagtaatt catccgcatc
    58321 ctgaccttct tcaatgtggc gcactaacct caggaagcga tgctgagttt tgttccagaa
    58381 gtcgatatcc acaaaactga aatcaccccg tgcacctttt ggatcggaga accacgcttc
    58441 tttcaatgca ctccgtaaca gactcagaat ccgtgaagcc gtttgcgcag ccagccgcag
    58501 cttcggtatc tggccttctt ttttatcgag cagcagcgga aaatggtgtt cgtaccaaca
    58561 gcgcgctttc atgttgtcaa aatcataacc aaatccccac aggcccactt ttgcctgctt
    58621 gagactgctg gcattaaaga gtttcaccac cagcgcggga agttccgtat tgttttctga
    58681 tttacccgtt tcgataaggc ctaaccagtc gcgccagatt aaaccgcctg gttgtggttt
    58741 gaccgagtaa aactcgccgc cctctttaag gggtacacgg taaggcgtta agggatgctg
    58801 ccacatggca taattcgcgc cgtaattttt ggtcgtcatc aggctcagga gcgcgtcgtt
    58861 ctgctcgccg caaatatcgc agttgccgac tgtcgtggta ttaaaatcaa tacgaatacg
    58921 ccgcggcatt ccccagtacg cctggagttt attaacctga tcatcggtta ccaccgcacc
    58981 ggccagttcg ctggtacgcg tcgggccaag ccaggggaaa accagatcgt caaatttttt
    59041 gggtagcggt aagtcggctt cgtcctgcgg catcacattg agccacagtt tgcgccacaa
    59101 gggcgtttgt tgattgccct gatattcctg taattcaatc agagtggtca tcggcccacc
    59161 gccgcgtaaa ccggtgcggt agcctttacc acctgacggc gcatttaact gtagggagaa
    59221 cagagctaac gcagaacaat gtgggcacag gtattccgtt acaccacgct tgataaagtg
    59281 gtctttatta aacttcgttg tttgagcgcc gggaatctca ggcagtagcg aagcgacctg
    59341 aactttatct cctgtgagcg cctcgaaatc ctgcataaat gaaggggaat ctggcccaaa
    59401 ctggaaagcg tgttctaagg acagcaatgc ttcccgtagc ttttcagatt ccagtccgtc
    59461 ttcccagata tcatcccaac gacgatgatc ttttggcgca aagctggttt gtagtagccc
    59521 cagcaaaaac tgccatgccg ctccctggag atctgcccgt ggcgcagcaa tatcgacaac
    59581 attttcatcc gccagatcga caggcgccag tttgcctgtc gttccgtctt tataacgaac
    59641 gggcaaccac ggggttgtca gaagtgaaaa cgagttcata cgcatcctct cgtaaaaaag
    59701 accatcccag gtcggttgtc catcattcca tccttttcgg tgtatcaact attcagagag
    59761 ccctgtcgca ggcatctcaa ccaaccttgc cagtcaatcc ttccatagcc gaatacccgc
    59821 aactttcgtc atcggtaact aaaatcacgt ttgccatctc cggatctttc cgctgttcaa
    59881 tgcaccactg cctgaacgct tccccttcca gtaaagaaaa ctcatcccga tgttttttcc
    59941 accagcttcg acgcactctg acaacgctca tttcccatgc gtgagcaccg ttggcataag
    60001 gcttcaccac actggcaata caggtagcca gccacaggga aacagactcc tccgccagac
    60061 gtgtcgacag cttttccgga aggtaatcgt tgatattggc ggcatagcca ggcctgaagt
    60121 tcagcacaaa ctttttagcc attgcgcgat cgcagtaata tttgcccact tgctcctgct
    60181 cgctgcgggc aaatccttcc ggcattacca cgtcctcacc gtagactgat tcaataagaa
    60241 ggcgggctgc gtgtggcatt tgaatagcgc cttgctcacg cagtacacgc tgcgtcagcc
    60301 agattcgtcc atgatcggga tagacatatg cactgttacg catggcactg ccgaaccatt
    60361 cgtcacctgg agcgtcgtcc cagacggggg ccagaatcag caattcagga ggggaacgct
    60421 cgtcttttcc gtcacgcttt aactgaccat taatatcgcg gatatgccgc tgtaatcgcc
    60481 ccgctcgctg gatcagcaaa tcaacagggg ccaggtcgga aatcatttcg tccatatcgc
    60541 aatcaacgct cgactctaag acctgagtac aaatgaggac ttttccggca cgctgtgagc
    60601 tatcttcttt accgaagcgt gccagcgttt ccgtttcaat tcgctggcga tcgctaaaag
    60661 caaagcggct atgaaacaga gaaaggctgg aagcaggaat gacgccgcgg gcaagcaact
    60721 gacgataaac cttaatagcg tcatcgacag aattccggat ccaggcgatg catttcccct
    60781 gacttactgc cgattcgata cgcgcaatac attcttgttc gctatgaagc caacccacgc
    60841 tcacgctacg ctcaacgtct ttgcgcgtcg ctacccggtg tgaatgcaca tcggatttcg
    60901 taacgtgcgt cagccagggg taatcatcct tttccaggaa cggagcttct tgctggccct
    60961 ctgtaccacg cgcaaaggca gcaacgagtt tgtcgcactg ctgttgagat aacgtggcag
    61021 aaagcaaaat gacgctgttt ccgccacgcg cctgccgctc gatcagccct tcaagaatgc
    61081 atgacatgta agcgtcacag gcatggatct catcagccag caggattttg ttactcagtc
    61141 ccagaagccg cagattatta tgtttgaacg gcatcacagc catcatcgcc tgatccagcg
    61201 tgccgacgcc aatttcagcc agtagcgcct tcttgttact gtcggcaaac caggccgcac
    61261 atccctgact gaacgtttgt tcatccggtt cttctgaccc gactaaatca ccggaccaga
    61321 gtgattcatt aaagcggtcc attaatgttc gggcactgtg tgccagcacc aggctggggc
    61381 gggactctgg cgaatagaaa gcaagccagg ttttgaccag tcgatcgtac atggcattgg
    61441 ccgttgccat tgttggcagg ccaaaaaaca aaccctgcgc tttacccgca gccatcaacc
    61501 tgtgcgccag gataagcgcc gcttccgttt tacctgcgcc agtcacatct tccagaataa
    61561 ataactgtgg ccctggctgg ctgatatcca gatccagcac cttttgctgt aacggtgtcg
    61621 gattctcaat aaaaggaaac agcgtattaa ttccggtgaa aggcgcgact tttgctttta
    61681 gaggaaagac ggttaaggcg ttttgagcct gaactaaagt tttctgccag taacctttaa
    61741 tatccattgg gtgtgcgacg cgtggaaaaa atcgcgttga cgaacccgtc cagtctgcga
    61801 gcacgacggt tgcagagata taccaggaaa ggtgttttat aagttcaatg ccctcatcat
    61861 catcccagaa tgcggggatc tctatgagcg gaaacagtgg tttgatttca agaagaaaat
    61921 ctcgcgcggc agctttgtct tcaggcagaa aattatccag ttcatcaata cggtcaggcg
    61981 ggcgaccatg atgcccggta gttatgggca tccacatctc catcacccgt ttaagtttgc
    62041 gcggagagag tgaagatgaa ggaagcaatt cttcgtattc acttaaataa tgatgccaca
    62101 gccagtagcc cagcgttgaa tgagagatct tttcgtaatt ctttcttgcg ccttccggaa
    62161 ccttgagttg tgggtgcagg taaagttgct gaaaagagcg ggcaaatttt ccagtatcgt
    62221 gccagcacaa tagccaggcg aaaaattgtg ctgcctgctc cgtatcagcg aaacctagtt
    62281 gacgaaaata gtcagtcagc ccgaagcaat tgcttttaac cattaaatag cccatcgcgg
    62341 ctacatccag cgaatgccag caaagaaggt gatagtcgtc gcccccgtct ttctcgccgc
    62401 gtcgggtttt tccccagaaa tcaaaaaaag tcacaatatt tttatccttc agtaaactta
    62461 aaggatattt acgcatatat taaaaattat ctgtgatata tatcgttcaa taaaatattt
    62521 ac
//
LOCUS       DAPOZO010000018        61246 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPOZO010000018 DAPOZO010000000
VERSION     DAPOZO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.018"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(129..1016)
                     /locus_tag="ROI21_001201"
     CDS             complement(129..1016)
                     /locus_tag="ROI21_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001484212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5110152.1"
                     /translation="MIDLRQLKYFVVVAEEEHVGRAAERLHISQSPLSRQIAQLEERL
                     GLTLFERNQQRIRLTADGQTFLAETRSLLTHANRLESLGKRLGRGEDGGLCIGYIENA
                     MHSGVLSTALRTLRVSRPNVHIALYSQPPATQIEGLRQRSLDFSLVDTPPTDSEQDLE
                     YIQVLDDRMLLALPQNHPLAQEENLVASQLGDEQWIAVIHNDDAHSRDHFVAACVNAG
                     LNPDIRLEAGEPLTALGLVAAGLGLALIQHSLRHNAPEGVVLRELPWMNYSTQLWAAW
                     HKANQRPLVSHFRQILRGE"
     gene            1149..2159
                     /locus_tag="ROI21_001202"
     CDS             1149..2159
                     /locus_tag="ROI21_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015368058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDX5110153.1"
                     /translation="MSIKNLLPGKIGLGGAPLGNMFRAIPEEEAHATVSEAWNLGVRY
                     FDTAPLYGSGLSEIRMGEALSQYPRDEYVLSSKVGRIMLDEMEDPAKRDFGEKGGLFE
                     HGLKNKILNDYSADATLRSIEDSLKRLKTDRLDIVWIHDPAQDFYGDSWLEQFNIART
                     GAFRALTRLRDEGVIKAWGLGVNRVEPCELTLALDEPKPDAFLLAGRYSLLDHERALQ
                     HLMPEALEQNVDIVVGGPYSSGVLAGGEHFEYQKASAAIQQKVAQINAIATRFNVNVK
                     AAALQFALANPAVAAVIPGSSRPGRMAEDLAALNAPVPAEFWAEMRRQNLVAENAPLP
                     TR"
     gene            2179..2976
                     /locus_tag="ROI21_001203"
     CDS             2179..2976
                     /locus_tag="ROI21_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX5110154.1"
                     /translation="MKLDLTGKVAVVSGSTSGIGQGIAAGLAKAGATVVVVGRKQRGV
                     DDAMAAIAAQTPTASLRGVVADLSTEDGVAALIAAVPTADVLVNNLGIFNDKDFFSVP
                     DDEWMHFYNVNVLSGVRLARHYAPGMTEQGWGRIIFVSSESGVAIPGDMINYGVTKAA
                     NLAVSHGLAKRLAGTGVTVNAILPGPTFTDGLKEMLADAAAQSGRSAREQADEFVKTS
                     RPSSIIQRAADVEEVANLAVYLASPLSSATTGAALRVDGGVVDTLTM"
     gene            3002..3418
                     /locus_tag="ROI21_001204"
     CDS             3002..3418
                     /locus_tag="ROI21_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HDX5110155.1"
                     /translation="MAKTTVSIEIPASADTVWQLMGGFDALPDWLPFIPKSVVTEGGR
                     VRSLTTSDGGTVVERLEAFDNRQRSYTYSIIQAPFPVVDYLSTITVHETADSQVSRVE
                     WFGEFTPVNVTNEEAEALFTGIYRDGLKALKDNFVA"
     gene            3576..3788
                     /locus_tag="ROI21_001205"
     CDS             3576..3788
                     /locus_tag="ROI21_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX5110156.1"
                     /translation="MKTIIAIFTASLFSVAAFNASAQSVTATGTTLDSAEAVIAQKAK
                     EMNASEYKITSARMGNTVTMSAELYK"
     gene            3837..4022
                     /locus_tag="ROI21_001206"
     CDS             3837..4022
                     /locus_tag="ROI21_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110157.1"
                     /translation="MPLKNRIVMPPMTRSRAGDVTTDMMADYYAQRASAGLIISEGTQ
                     ISRSAAHNFPWHADLLR"
     gene            complement(4249..5481)
                     /gene="nadR"
                     /locus_tag="ROI21_001207"
     CDS             complement(4249..5481)
                     /gene="nadR"
                     /locus_tag="ROI21_001207"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDX5110158.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(5502..6884)
                     /gene="radA"
                     /locus_tag="ROI21_001208"
     CDS             complement(5502..6884)
                     /gene="radA"
                     /locus_tag="ROI21_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDX5110159.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(6933..7901)
                     /gene="serB"
                     /locus_tag="ROI21_001209"
     CDS             complement(6933..7901)
                     /gene="serB"
                     /locus_tag="ROI21_001209"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDX5110160.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            8007..8648
                     /locus_tag="ROI21_001210"
     CDS             8007..8648
                     /locus_tag="ROI21_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDX5110161.1"
                     /translation="MARTKLKFWLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESIEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEESEKKE"
     gene            8676..9692
                     /gene="lplA"
                     /locus_tag="ROI21_001211"
     CDS             8676..9692
                     /gene="lplA"
                     /locus_tag="ROI21_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDX5110162.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAIREAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRTQVFTDSLTPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            9724..9987
                     /locus_tag="ROI21_001212"
     CDS             9724..9987
                     /locus_tag="ROI21_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX5110163.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(10148..10867)
                     /gene="deoD"
                     /locus_tag="ROI21_001213"
     CDS             complement(10148..10867)
                     /gene="deoD"
                     /locus_tag="ROI21_001213"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDX5110164.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(10924..12147)
                     /gene="deoB"
                     /locus_tag="ROI21_001214"
     CDS             complement(10924..12147)
                     /gene="deoB"
                     /locus_tag="ROI21_001214"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDX5110165.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(12199..13521)
                     /gene="deoA"
                     /locus_tag="ROI21_001215"
     CDS             complement(12199..13521)
                     /gene="deoA"
                     /locus_tag="ROI21_001215"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDX5110166.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(13599..14378)
                     /gene="deoC"
                     /locus_tag="ROI21_001216"
     CDS             complement(13599..14378)
                     /gene="deoC"
                     /locus_tag="ROI21_001216"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDX5110167.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14636..16186
                     /locus_tag="ROI21_001217"
     CDS             14636..16186
                     /locus_tag="ROI21_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDX5110168.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARHALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLEDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANISGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            16158..17021
                     /locus_tag="ROI21_001218"
     CDS             16158..17021
                     /locus_tag="ROI21_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDX5110169.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(17138..17917)
                     /locus_tag="ROI21_001219"
     CDS             complement(17138..17917)
                     /locus_tag="ROI21_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDX5110170.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYGALGLHPGMLEKHSDASLEQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRLEPADEIAEVLLNNTYTLFNVL"
     gene            complement(17914..18987)
                     /locus_tag="ROI21_001220"
     CDS             complement(17914..18987)
                     /locus_tag="ROI21_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDX5110171.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(19109..19270)
                     /locus_tag="ROI21_001221"
     CDS             complement(19109..19270)
                     /locus_tag="ROI21_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDX5110172.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(19397..20002)
                     /gene="osmY"
                     /locus_tag="ROI21_001222"
     CDS             complement(19397..20002)
                     /gene="osmY"
                     /locus_tag="ROI21_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDX5110173.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(20395..21984)
                     /gene="prfC"
                     /locus_tag="ROI21_001223"
     CDS             complement(20395..21984)
                     /gene="prfC"
                     /locus_tag="ROI21_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDX5110174.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(22077..22754)
                     /gene="yjjG"
                     /locus_tag="ROI21_001224"
     CDS             complement(22077..22754)
                     /gene="yjjG"
                     /locus_tag="ROI21_001224"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDX5110175.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(22769..23215)
                     /gene="rimI"
                     /locus_tag="ROI21_001225"
     CDS             complement(22769..23215)
                     /gene="rimI"
                     /locus_tag="ROI21_001225"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDX5110176.1"
                     /translation="MNTISSLETADLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(23184..23597)
                     /gene="holD"
                     /locus_tag="ROI21_001226"
     CDS             complement(23184..23597)
                     /gene="holD"
                     /locus_tag="ROI21_001226"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDX5110177.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VESPALTELRANPTARAALWQQICTYEHDFFPRNG"
     gene            23700..24731
                     /gene="rsmC"
                     /locus_tag="ROI21_001227"
     CDS             23700..24731
                     /gene="rsmC"
                     /locus_tag="ROI21_001227"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDX5110178.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            24999..25085
                     /locus_tag="ROI21_001228"
     tRNA            24999..25085
                     /locus_tag="ROI21_001228"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25033..25035,aa:Leu,seq:cag)
     gene            25114..25200
                     /locus_tag="ROI21_001229"
     tRNA            25114..25200
                     /locus_tag="ROI21_001229"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25148..25150,aa:Leu,seq:cag)
     gene            25235..25321
                     /locus_tag="ROI21_001230"
     tRNA            25235..25321
                     /locus_tag="ROI21_001230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25269..25271,aa:Leu,seq:cag)
     gene            complement(25360..25596)
                     /locus_tag="ROI21_001231"
     CDS             complement(25360..25596)
                     /locus_tag="ROI21_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDX5110179.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            25737..26525
                     /gene="fhuF"
                     /locus_tag="ROI21_001232"
     CDS             25737..26525
                     /gene="fhuF"
                     /locus_tag="ROI21_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDX5110180.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLSTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(26563..27240)
                     /gene="bglJ"
                     /locus_tag="ROI21_001233"
     CDS             complement(26563..27240)
                     /gene="bglJ"
                     /locus_tag="ROI21_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDX5110181.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(27198..27923)
                     /locus_tag="ROI21_001234"
     CDS             complement(27198..27923)
                     /locus_tag="ROI21_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDX5110182.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            28543..29313
                     /locus_tag="ROI21_001235"
     CDS             28543..29313
                     /locus_tag="ROI21_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDX5110183.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFLASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            29304..29777
                     /locus_tag="ROI21_001236"
     CDS             29304..29777
                     /locus_tag="ROI21_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDX5110184.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            29885..30424
                     /gene="dnaT"
                     /locus_tag="ROI21_001237"
     CDS             29885..30424
                     /gene="dnaT"
                     /locus_tag="ROI21_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDX5110185.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     ITPARLAELLALEEKLARPGSDVALDDQLYQEPQTAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALRESVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            30427..31164
                     /gene="dnaC"
                     /locus_tag="ROI21_001238"
     CDS             30427..31164
                     /gene="dnaC"
                     /locus_tag="ROI21_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDX5110186.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            31213..31707
                     /locus_tag="ROI21_001239"
     CDS             31213..31707
                     /locus_tag="ROI21_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDX5110187.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSNGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            31961..34252
                     /gene="opgB"
                     /locus_tag="ROI21_001240"
     CDS             31961..34252
                     /gene="opgB"
                     /locus_tag="ROI21_001240"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDX5110188.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLAAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDIMKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWALELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(34391..35413)
                     /locus_tag="ROI21_001241"
     CDS             complement(34391..35413)
                     /locus_tag="ROI21_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDX5110189.1"
                     /translation="MSTMKVLICQEIKNLVWEVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APSEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            35552..36457
                     /locus_tag="ROI21_001242"
     CDS             35552..36457
                     /locus_tag="ROI21_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDX5110190.1"
                     /translation="MSRSQNLRHSVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAASVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(36628..38280)
                     /locus_tag="ROI21_001243"
     CDS             complement(36628..38280)
                     /locus_tag="ROI21_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDX5110191.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRARTENLDNELIMETGARVIGYMRHSEEDMSPNDNSLHMQRAKLERTFNL
                     AALNAKRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
     gene            38971..39942
                     /locus_tag="ROI21_001244"
     CDS             38971..39942
                     /locus_tag="ROI21_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HDX5110192.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            40006..40479
                     /locus_tag="ROI21_001245"
     CDS             40006..40479
                     /locus_tag="ROI21_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDX5110193.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVVTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVALLSIALLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLINDTKQSDSGDKMQ"
     gene            40476..41747
                     /locus_tag="ROI21_001246"
     CDS             40476..41747
                     /locus_tag="ROI21_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDX5110194.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEMVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAVAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(41790..43454)
                     /gene="tsr"
                     /locus_tag="ROI21_001247"
     CDS             complement(41790..43454)
                     /gene="tsr"
                     /locus_tag="ROI21_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDX5110195.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFLIQQQQQQQREASAVVKTVTPATPRKMAVADSEENWETF"
     gene            43831..45981
                     /gene="btsT"
                     /locus_tag="ROI21_001248"
     CDS             43831..45981
                     /gene="btsT"
                     /locus_tag="ROI21_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDX5110196.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVIPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            46099..46302
                     /locus_tag="ROI21_001249"
     CDS             46099..46302
                     /locus_tag="ROI21_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDX5110197.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            46313..47269
                     /gene="yjiA"
                     /locus_tag="ROI21_001250"
     CDS             46313..47269
                     /gene="yjiA"
                     /locus_tag="ROI21_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDX5110198.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(47315..48229)
                     /gene="mrr"
                     /locus_tag="ROI21_001251"
     CDS             complement(47315..48229)
                     /gene="mrr"
                     /locus_tag="ROI21_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine/cytosine-specific restriction
                     endonuclease Mrr"
                     /protein_id="HDX5110199.1"
                     /translation="MTVPTYDKFIEPVLRYLATKPEGAAARDVHEAAADALGLDDSQR
                     SKVITSGQLVYKNRAGWAHDRLKRAGLSQSLSRGKWCLTPAGFDWVASHPQPMTEQEM
                     NHLAFNFVNVKLKSRPDAVDLDPKADSPDHEELAKSSPDDRLDQALKELRDAVADEVL
                     ENLLQVSPSRFEVIVLDVLHRLGYGGHRDDLQRVGGTGDGGIDGVISLDKLGLEKVYV
                     QAKRWQNTVGRPELQAFYGALAGQKAKRGVFITTSGFTSQARDFAQSVEGMVLVDGER
                     LVHLMIENEVGVSSRLLKVPKLDMDYFE"
     gene            48417..51929
                     /gene="hsdR"
                     /locus_tag="ROI21_001252"
     CDS             48417..51929
                     /gene="hsdR"
                     /locus_tag="ROI21_001252"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     endonuclease"
                     /protein_id="HDX5110200.1"
                     /translation="MMNKSNFEFLKGVNDFTYAIACAAENNYPDDPNTTLIKMRMFGE
                     ATAKHLGLLLNIPPCENQHDLLRELGKIAFVDDNILSVFHKLRRIGNQAVHEYHNDLD
                     DAQMCLRLGFRLAVWYYRLVTKDYDFPVPVFVLPERGENLYHQEVLTLKQQLEQQARE
                     KAQTQAEVEAQQQKLVALNGYIAILEGKQQETEAQTQARLAALEAQLAEKNAELAKQT
                     EQERKAYHKEITDQAIKRTLNLSEEESRFLIDAQLRKAGWQADSKTLRFSKGARPEPG
                     VNKAIAEWPTGKDETGNQGFADYVLFVGLKPIAVVEAKRKNIDVPGKLNESYRYSKCF
                     DNGFLRETLLEHYSPDEVHEAVPEYETSWQDTSGQQRFKIPFCYSTNGREYRAAMKTK
                     SGIWYRDVRDTRNMSKALPEWHRPEELLEMLGSEPQKQNQWFADNPGMSELGLRYYQE
                     DAVRAVEKAIVKGQQEILLAMATGTGKTRTAIAMMFRLIQSQRFKRILFLVDRRSLGE
                     QALGAFEDTRINGDTFNSIFDIKGLTDKFPEDSTKIHVATVQSLVKRTLQSDEPMPVA
                     RYDCIVVDEAHRGYILDKEQTEGELQFRSQLDYVSAYRRILDHFDAVKIALTATPALH
                     TVQIFGEPVYRYTYRTAVIDGFLIDQDPPIQIITRNAQEGVYLSKGEQVERISPQGEV
                     INDTLEDDQDFEVADFNRGLVIPAFNRAVCNELTNYLDPTGSQKTLVFCVTNAHADMV
                     VEELRTAFKKKYPQLEHDAIIKITGDADKDARKVQTMITRFNKERLPNIVVTVDLLTT
                     GVDIPSICNIVFLRKVRSRILYEQMKGRATRLCPDVNKTSFKIFDCVDIYSTLESVDT
                     MRPVVVRPKVELQTLVNEITDSETYKITEADGRSFAEHSHEQLVAKLQRIIGLATFNR
                     DRSETIDKQVRRLDELCQDAAGVNFNGFASRLREKGPHWSAEVFNKLPGFIARLEKLK
                     TDINNLNDAPIFLDIDDEVVSVKSLYGDYDTPQDFLEAFDSLVQRSPNAQPALQAVIN
                     RPRDLTRKGLVELQEWFDRQHFEESSLRKAWKETRNEDIAARLIGHIRRAAVGDALKP
                     FEERVDHALTRIKGENDWSSEQLSWLDRLAQALKEKVVLDDDVFKTGNFHRRGGKAML
                     QRTFDDNLDNLLDKFSDYIWDELA"
     gene            52131..53720
                     /gene="hsdM"
                     /locus_tag="ROI21_001253"
     CDS             52131..53720
                     /gene="hsdM"
                     /locus_tag="ROI21_001253"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     methyltransferase"
                     /protein_id="HDX5110201.1"
                     /translation="MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETG
                     QEAEYLPEGYRWDDLKSRIGQEQLQFYRKMLVHLGEDDKKLVQAVFHNVSTTITEPKQ
                     ITALVSNMDSLDWYNGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTII
                     HLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELV
                     PGTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTN
                     ITRTFVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHL
                     HTILRLPTGIFYAQGVKTNVLFFTKGTVANPNQDKNCTDDVWVYDLRTNMPSFGKRTP
                     FTDEHLQPFERVFGEDPHGLSPRTEGEWSFNAEETEIADSEENKNTDQHLATSRWRKF
                     SREWIRTAKSDSLDISWLKDKDSIDADNLPEPDVLAAEAMGELVQALSELDALMRELG
                     ASDEADAQRQLLEEAFGGVKE"
     gene            53717..55084
                     /gene="hsdS"
                     /locus_tag="ROI21_001254"
     CDS             53717..55084
                     /gene="hsdS"
                     /locus_tag="ROI21_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     specificity subunit"
                     /protein_id="HDX5110202.1"
                     /translation="MSAGKLPEGWEQIEIGDIADVISGGTPKSGVAENFAPSGEGVAW
                     LTPADLSGYKEKYISHGARDLTTLGYSSCSAKLMPKGTILFSSRAPIGYVAIAANEIA
                     TNQGFKSFAFPSDIFPDYAYYFLRNIRHIAEEMGTGTTFKEISGSSAKTLPFVLVPFA
                     EQKIIAEKLDTLLAQVDSTKARLEQIPQILKRFRQAVLGAAVRGKLTEDWRDNSSLSG
                     WREGKLGEFIKKPSYGTSSKSNKEGLIPVLRMGNLQGGKLDWTDLVYTSDTIEIEKYK
                     LEYNDVLFNRTNSPELVGKTAIYKSEQPAIYAGYLIRVQCLPDLNPDYLNYHLNSILG
                     RQYCYSVKSDGVSQSNINAQKLIAYPITVPPLPEQAEIVRRVEQLFAYADTIEKQVNN
                     ALARVNNLTQSILAKAFRGELTAQWRAENPELISGENSAAALLEKIKAERAASGGKKA
                     SRKKS"
     gene            55313..55654
                     /gene="symE"
                     /locus_tag="ROI21_001255"
     CDS             55313..55654
                     /gene="symE"
                     /locus_tag="ROI21_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HDX5110203.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAIDVKVMEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
     gene            complement(55701..57794)
                     /locus_tag="ROI21_001256"
     CDS             complement(55701..57794)
                     /locus_tag="ROI21_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDX5110204.1"
                     /translation="MKATEARLLDFLKRSQQFVITIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQITKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVAFGQQGYSEYFDSFMRHYLTVKTGEIPRTDEV
                     YEAFKLHARSQSVAEKGVDRLVEDIHIYAEYYCAMALGKESDKSLATAFQDLRELKVD
                     VAYPFLLALYHDYKNGVLSHEDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVI
                     NKEKYLESIQVHFLNLPSYRRFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRER
                     VEEFTIEHIMPQNENLSAKWREELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAE
                     KRDIEDGFKHSPLYLNIGLGQCEKWDEAAIRARADRLADLAVQVWQAPALPEEVLAVY
                     RAQPENKTSYSLSDYPFLADGSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETN
                     FVDVVPQKSRLRLSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMG
                     LIRQAFEKQMESALV"
     gene            complement(57892..57972)
                     /locus_tag="ROI21_001257"
     CDS             complement(57892..57972)
                     /locus_tag="ROI21_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110205.1"
                     /translation="MQTRCVLQQVRDERLKDIGLCREDVV"
     gene            complement(58200..59120)
                     /locus_tag="ROI21_001258"
     CDS             complement(58200..59120)
                     /locus_tag="ROI21_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDX5110206.1"
                     /translation="MTNFTTSTPHDALFKSFLTHSDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFNEFISEITRRLPQQRERIMTIAERIHNDGYIKGEQRILRLFLQNGVDPEW
                     IQKITGLSAEQIQALEQPLPEREPYSWLKS"
     gene            59305..60585
                     /locus_tag="ROI21_001259"
     CDS             59305..60585
                     /locus_tag="ROI21_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDX5110207.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDHATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            60701..61060
                     /locus_tag="ROI21_001260"
                     /pseudo
     CDS             60701..61060
                     /locus_tag="ROI21_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313325.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
BASE COUNT        15158 a        16126 c        15209 g        14753 t
ORIGIN      
        1 ccagcatcgc atccggtatt agttaaatga tgagtccccg aaaaggcaga attctcttta
       61 agcgtattag ctcagatgcg gactgacatc tcaaatcaag cgggaactca tggttcccgc
      121 ttaacgtttt attcaccgcg caaaatctgg cggaaatggg aaaccagcgg acgttggttg
      181 gctttatgcc aggctgccca taattgcgtt gaataattca tccacggcaa ttcacgtaat
      241 accacccctt ccggcgcatt atgacgcaga ctatgttgaa ttaaggccag acctaatccc
      301 gcagcgacca aacctaatgc cgtgaggggt tcacccgctt ccaggcgaat atcaggatta
      361 agcccggcgt taacacaggc ggcgacaaaa tgatcgcgag aatgcgcatc gtcgttatga
      421 atcaccgcaa tccactgctc atcgccaagt tgtgacgcga caaggttttc ctcctgcgcc
      481 agaggatgat tttgcggtaa ggccaacaac attcgatcat ccagaacctg aatatattcc
      541 agatcctgct cgctgtcggt gggcggcgta tcgaccagag aaaaatcgag actgcgctgg
      601 cgcaggcctt caatctgggt agccggtggt tggctgtaaa gtgcgatatg cacattcgga
      661 cgcgatacac gtaacgtacg tagcgccgta gaaagcacac cggaatgcat ggcgttttcg
      721 atatagccaa tacacaagcc accatcttcc ccccgtccca ggcgtttgcc taacgactcc
      781 aggcggttgg cgtgtgtcag caacgaacgt gtttcggcaa ggaatgtctg tccatcagcg
      841 gtcaggcgaa tgcgctgctg attacgctca aacagcgtca gccccagccg ttcttccagt
      901 tgtgcaatct ggcggctcag cggtgattgc gagatatgta atcgctcggc tgcgcgtccc
      961 acgtgctctt cttctgcgac caccacgaag tacttcagtt ggcgtaaatc aatcatgtta
     1021 gacctcaaag gacttaagtc agacgaatta tgtcctatca agtcacaaca tcgcaaggta
     1081 ttgtgatcca cgtcacaggg tgatagcaaa aaacgcccta acccaaaccg aataattgag
     1141 gacatcttat gagcatcaaa aatttacttc ccggcaaaat tggcttaggc ggcgcaccac
     1201 tcggcaacat gttccgtgcc attccggaag aagaggctca cgccaccgtg agcgaagcct
     1261 ggaacctggg tgtgcgttac tttgataccg cgccgctgta cggctctggc ctttctgaaa
     1321 tccgcatggg tgaagcgctg tctcagtatc cacgcgatga atacgttctg agtagcaaag
     1381 tcggtcgcat catgctggac gaaatggaag atccggcaaa acgggatttc ggtgaaaaag
     1441 gtggcctgtt cgaacacggc ctgaaaaaca aaattctgaa tgactactct gctgacgcca
     1501 cgctgcgctc gattgaagac agcctgaaac gcctgaaaac agaccgtctg gacattgtct
     1561 ggatccacga cccggcgcag gacttctacg gtgacagctg gctggagcag ttcaacattg
     1621 ctcgtaccgg tgcgtttcgt gcactgacac gtttgcgtga cgaaggtgtg attaaagcct
     1681 ggggactagg tgtgaaccgc gtcgaacctt gcgaactgac gctggcgctg gacgaaccga
     1741 agccagatgc tttcctgctg gcggggcgtt attccctgct ggatcacgaa cgtgcgctgc
     1801 aacacctgat gccagaagca ctggaacaga acgtggatat cgttgtgggc ggtccataca
     1861 gctctggcgt actggcaggc ggcgagcact ttgaatatca gaaagcctct gcggccatcc
     1921 agcagaaagt ggcgcaaatt aacgccattg ccacacgttt caatgtgaac gttaaagcag
     1981 ctgcgctgca gttcgcgctg gcaaacccgg ccgttgcagc ggttattcct ggctccagcc
     2041 gtcctggccg tatggcagaa gatctggctg cactgaatgc gcctgttccg gctgaattct
     2101 gggcagaaat gcgccgtcaa aatctggttg cggaaaacgc tccgctgccg acccgttaat
     2161 caacctggtg agaaaacgat gaaactggat ctgacaggta aagtcgcggt ggtcagcggc
     2221 tcaacctccg gcattgggca gggtatcgcc gccggactgg caaaagcagg cgcaacggtg
     2281 gttgtcgtcg gccgtaaaca gcgcggcgtt gatgacgcca tggcagcgat tgcggcacaa
     2341 actcccacgg cgtcactgcg tggcgtggtg gccgatctca gcaccgaaga cggcgtggct
     2401 gcgctgatcg ctgcagtccc aacggctgat gtgctggtca ataatctggg catttttaac
     2461 gataaagatt tctttagcgt tccggatgat gagtggatgc acttctataa cgtcaacgtc
     2521 ctctccggcg ttcgtctggc gcgtcactat gcgccaggca tgacggaaca gggctggggg
     2581 cggattatct ttgtctcgtc cgaatccggc gtggcaattc ctggcgacat gatcaactac
     2641 ggcgtcacca aagctgcgaa tctggcggtc tctcatggtc tggcgaaacg actggcgggg
     2701 accggtgtga ccgtcaacgc cattctgcca gggccaacct ttaccgatgg cctgaaagag
     2761 atgctggcgg atgccgctgc acaatcggga cgcagtgcgc gtgaacaggc cgatgagttt
     2821 gttaaaacct cacgtccgag ctccatcatc cagcgtgcgg cagatgtgga agaagtggcg
     2881 aatctggcgg tttatctcgc gtcaccgctt tcctcggcaa ccacaggggc agccctgcgc
     2941 gtcgatggcg gcgtcgtcga caccctgaca atgtaaccct ataaattaag agaaaattat
     3001 catggctaaa acaactgtat cgatcgaaat tccggcttct gccgacaccg tatggcaatt
     3061 gatgggcggc tttgatgcgc tgccagactg gctgccgttc atcccgaaaa gcgtagtgac
     3121 agaaggcggt cgcgttcgtt cgctgacaac ctctgacggc ggcactgtgg ttgaacgtct
     3181 ggaagcgttt gataaccgtc aacgcagtta tacctattcc attattcagg caccgttccc
     3241 ggtggtggat tacctctcca ccattaccgt tcatgaaacc gccgacagcc aggtttcccg
     3301 tgtggaatgg ttcggtgaat tcaccccggt gaatgtgacg aacgaagagg ccgaggcgct
     3361 gtttaccggc atttatcggg atggtctcaa ggcgctgaag gacaactttg ttgcctgatg
     3421 cgatcgacag gcgtcggaac gtccggcgtc tgattattaa caaaaacaga atagtctatt
     3481 gtcatttgta tcatttatgt tatcaggcag caggcgtatt ctctccccat cgcgttaata
     3541 aacgccaaac aattaagaca ctctggaata taaacatgaa aacgatcatc gcaattttta
     3601 cagcttctct tttctctgtc gcggcgttta acgcatcggc gcaaagcgtg acagcaaccg
     3661 gtacaacact ggattctgcg gaagcagtta ttgcgcagaa agcgaaagag atgaacgcct
     3721 ctgaatacaa aatcaccagc gctcgcatgg gcaataccgt caccatgagt gccgaactgt
     3781 acaaataacc gcgcaaggga cccattacgt aatttgctaa atatgcgctg aatgcgttgc
     3841 cattaaaaaa ccgtattgta atgccaccga tgacacgttc ccgtgcgggt gacgttacga
     3901 ctgacatgat ggcggactat tacgcgcagc gcgccagtgc aggacttatc atcagcgaag
     3961 gtacgcaaat cagcagaagc gctgcgcata attttccatg gcacgctgat ttactccggt
     4021 aaatacacca aagcgcgcgc cgaagcggac tgtcagattt gattgcattc tggctacgca
     4081 actggtcggt tggagtatga agttgctcac gaatagcctt agcatctgca atttcgtagc
     4141 gggtcacccg tgcaggttct ttctaatctc aacagcctct tccagagagt ttttcaactc
     4201 atcaaaaaaa ctcaatttat acctcgcctt tgagccgttt cgtcacggtt atctttgctc
     4261 ccccatcatc tcccgcacaa gttccacgca gcgcaggaaa cggctgtcgt aatcgtcctc
     4321 ttcaacccgc acgaattcga tattgttctc ttccagcatc tccaccagca agttctggaa
     4381 ctctttgcga tccaccgaac tgccgaggct gcgtaaacca tccgccaccc acggggtgtt
     4441 gttctccagc aagataacca gatcaaaacg gtattcatcg atcagcgcct gcacgaacgg
     4501 atgctcccgc ccttcgtact ttttgcagaa cgcctgggta gtgacaaaat cagtgtcaat
     4561 aaacgccact ttattggcat atttcactgc aaaatcaata tattgagcgt gtcccagcgc
     4621 gattttatcg tagtcagaat actgcaatgc gatctcatca ccgccgaggt gtgaaaagac
     4681 ataatcgcga ccatattccc acgcactggt ggtattgaag atattggcaa gtttgtttac
     4741 cagggtggat ttaccgctcg actcgccgcc aaggatcgcc acggtacgca caaaaaacgg
     4801 cttcacttca gtggggatat attcccagta gcggaacggg ttttcgcgga tctgcgcacc
     4861 gctgatactc ataaaggtgc gtttcggatc gaccagcacc gtttcgatcc ccagatgttc
     4921 catatactgt ggcgcatcgg cttcttccga ggtgtagatc aggtccggct ggatcccctt
     4981 ttcagccata aactttttga tgccgttgct ccatacatcc cagccgtgcg gatatggctc
     5041 catgccctct tcattgaaag catgaatacg gatatttttc tgatatttaa aggtttgcaa
     5101 taaccagcgc agacgatccg gcacggtggg ctgctgcgac atggcgctgt cttcaaacaa
     5161 cgcgcggtcg cgggtatcgt caaaacccat aatgatatgc agctcgtcaa cctggctaca
     5221 ggcgcgctgg ataaggtaga tatgtccggt atgcagcggg tagaacttac cgaataccac
     5281 gccaatggtt ttcttttggc ggggaaactc aagccccaaa aaacggtgca acgcctccag
     5341 tttttgcgcg ctggggcttt taattttggc attcagtaac tggcttaaat accctttggt
     5401 cataccgctg gcatcagcta cctgctgtag cgtgcagccc tgttgcttga tggcagtttt
     5461 caggtaatca aatgacgaca tatctccctc cgtatctctc attataagtc gtcgaacacg
     5521 ctaagcgcgt cggagagttt tttaacgcca aaaatctgca tcccttccgg cgcttttttc
     5581 ggcacattag ccgccggaac aatcgcccgg cgaaaaccgt gtttcgccgc ttcagagatt
     5641 cgttcctgcc cgctgggcac cgggcggatc tcccctgcca gcccgacttc accaaacacc
     5701 accagatcct gcggtagcgg tctgtcacgc aggctggaaa ccatcgccag cagtaacgct
     5761 aagtcggcgc tggtttccgt taccttcacg ccgccgacca cgttcacaaa tacatcctga
     5821 tcggccattt gcagcccacc gtgacggtgc aagacagcca gcaggattgc cagacggttt
     5881 tgttccagac ctactgccac gcgacgtgga ttcgccatca tcgagtgatc gaccaatgcc
     5941 tggatctcca ccagcagcgg acgcgttcct tcccatacca ccatcactga gctaccggag
     6001 gtcacttcat caccgcggct taaaaagatg gcagaagggt tgctgacttc acgcagcccc
     6061 tgttcggtca tcgcgaagac gcccagctca ttcaccgcgc cgaagcggtt tttatggctg
     6121 cgtaaggtgc gaaaacggga gtcggcatcg ccatccagaa gcaccgaaca gtcgatacag
     6181 tgttccagca ctttcgggcc agccagcgag ccatctttgg ttacgtgccc aaccatgaca
     6241 atcgccacgc cgcgcgtttt ggcgaagcgc gtcagataag ccgccgtttc acgcacctgc
     6301 gccacgctgc caggcgatga ctgaacatcc gccatatgca tcacctggat ggagtcaatt
     6361 accatcagct tcggttgctc ttcttcggca atcaggcaga tctgctcgat gctggtttcc
     6421 gacaacatat tgagattgtc agtcggcagg ccaaggcgat gagcgcgcat tgccacctgt
     6481 tgcagcgact cttcgccggt gacatacagc gttttcatct gctgggccag tttgcacagc
     6541 gtttgcaaca gcaatgtgga tttcccggca cctgggttac cgccaatcag aatggcactt
     6601 cccggcacca cgccgccgcc tagtacgcgg tcgaactctt tgaatccggt ggaaaaacgc
     6661 ggcagctctt caaggctgat atcggagagt ttctggactt ttgctacccc ggcgctaccg
     6721 gcatagccac tgagacgctc gttacgcgcc accgttggcg acgctgcaag acgcacctcg
     6781 gtgatggtgt tccaggcatg acaggcgctg cactgcccct gccagcgcgg ataatcggcc
     6841 ccgcattcat tacaaacaaa ggcgcgtttt ggagcttttg ccacggtgta cctcgttaat
     6901 gctgtgccgc ccgcaggatg acgggcgagc aattacttct gattcaggct gccagagagg
     6961 atgcagaata cccccatcag gtcagcgtga cggatggtga cttccgcctt ttcattcact
     7021 tttggcttgg catggtaggc aatccccagc cctgccgctt tgatcatcgg caggtcattg
     7081 gctccatcgc caatcgccac ggtctgcgcc agcgggattt catactcctg cgcgaggcgg
     7141 gtcagggttt tcgctttgta ctgcgcgtct acgatgtcgc cgatcacatt gccggtaaat
     7201 ttaccgtcca tgatctccag ttcattggct accacggctg tcaggcgcag cttgtcgcgc
     7261 agatattcag caaagaaagt aaagccgccg gaagcaatcg ccactttcca gcccagcgtt
     7321 tccagcttga gcaccagttg cgttaagcct ggcatcagcg gcagattttc acgcacctgt
     7381 tgcagaatat tggcatcagc gcctttcagc gtcgccacgc ggctgcgcag gctggcggta
     7441 aaatcaagtt cgccgcgcat cgcccgttcg gttacctccg ccaccatctc gcccgttccg
     7501 gccagtttgg caatttcatc aatacattca atctggatgg cggtggaatc catgtccatc
     7561 accagcaaac ccggcgtgcg caggtgcggg attttcccca gcggcgcgac atccagctgc
     7621 gcttcgtggg ccaggcgcgt agcccgcgcg gtgagtgaac ctgccagacg aatcacctga
     7681 taatcttcca cgcaccaggc ggcaacaatc accatcgccg cgcccagttt gctctggtat
     7741 tgggtcagac gttgtttatc cagcccacga ccatacagca gccagccgct acgacctgcg
     7801 tggtaatcca gtggcatcac ttcatcacca cttaatgaaa gaggcagacc gggccataaa
     7861 gagacatctt caggcaggtc gcaccaggta atgttaggca ttaaggctcc tgtaaaatcg
     7921 ttcgaagcag ggaaaataac gcatgaggct accttgtatc cattgcttct ggcaacatta
     7981 agtctcaaat tttcaaaggg tggaagatgg ctcgcacaaa actgaaattc tggctgcatc
     8041 gggcagtgat tgtcctgttc tgtcttgcct tgttagtggc gctgatgcag ggggcgtcat
     8101 ggtttagtca aaaccaccag cgacagcgta atccgcagct ggaagaactg gcccgcaccc
     8161 tggcgcgtca ggtgacgctg aacgttgcac cgctgatgcg taccgactca ccggatgaaa
     8221 aacgcattca ggcgatcctc gatcagttaa cggatgaaag ccgtatcctc gacgcgggtg
     8281 tgtatgacga acaaggcgat cttatcgcac gttctggcga aagcatcgaa gtgcgcgacc
     8341 ggttggcgct cgacggcaaa aaagcgggcg gctattttaa ccagcagatt gtcgagccaa
     8401 ttgcgggtaa aaacggaccg ctcggctatc tgcgcctgac gctcgacacc catacgctcg
     8461 ccaccgaagc ccaacaggtg gataacacca ctaacatttt acgcctgatg ttgctgctct
     8521 cgctggcaat cggcgtagtg ctgacccgca cgctgctaca gggtaaacgc acccgctggc
     8581 agcaatcgcc cttcctgtta accgccagca aaccggtgcc ggaagaagaa agcgagaaaa
     8641 aagagtgacc cattactaca agaaaggaaa tcgttatgtc cacattacgc ctgctcatct
     8701 ctgactctta cgacccgtgg tttaacctgg cggtggagga gtgtattttt cgccagatgc
     8761 ccgccacgca gcgcgttctg tttctctggc gcaatgccga cacggtagtg attggtcgcg
     8821 cacagaaccc gtggaaagag tgtaataccc ggcggatgga agaagataac gtccgcctgg
     8881 cgcggcgcag cagcggtggc ggcgcggtgt tccacgatct cggtaatacc tgctttacct
     8941 ttatggctgg caagccggag tacgataaaa ccatctccac gtcgattgtg ctcaatgctc
     9001 tgaacgcgct cggcgtcagc gccgaagcgt ccggacgtaa cgatctggtg gtgaaaaccg
     9061 ccgaaggcga ccgcaaagtc tcaggctcag cctatcgcga aaccaaagat cgcggcttcc
     9121 atcacggcac cttgctactc aatgctgacc ttagccgtct ggcaaactat cttaacccgg
     9181 ataaaaagaa actggcggcg aaaggcatta cctcggtacg ttcccgcgtg accaatctga
     9241 ccgaattgct gccagggatc acccatgagc aggtttgcga ggcgattcga gaggcctttt
     9301 tcgcccatta tggcgagcgc gtggaagcag aaatcatctc cccggacaaa acgccagact
     9361 tgccaaactt cgccgaaacc tttgcccgcc agagtagctg ggaatggaac ttcggtcagg
     9421 ctccggcatt ctcgcatctg ctggatgaac gctttacctg gggcggtgtg gaactgcatt
     9481 tcgacgttga aaaaggccat atcacccgca cccaggtgtt taccgacagc ctcacccccg
     9541 cgccgctgga agccctcgcc ggacgactgc aaggctgcct gtaccgcgca gatatgctgc
     9601 aacaggagtg cgaagcgctg ttggttgact tcccggaaca ggaaaaagag ctacgggagt
     9661 tatcggcatg gatggcgggg gcggtaaggt aactcgctga ttaaaatatc cagggaagga
     9721 aatatgatcg atctggaaaa ccaggaacgg gagattatca atttaatgtt cagccagcgt
     9781 atttcctggc tagccgccgt gcgtatcagg cataaattat cgcttgctga agtaagtaaa
     9841 atgttaggta tctcgattaa ttctctaaaa cagatcgaaa agacggaacg gcttagctca
     9901 aacatcaaaa gcaaaatggc agaaatttat ggttgtccac cagagttgct gatatgccct
     9961 tcctggatga cggctgaaca taaatgaata gattcgtgaa tagatacttg aatggatttt
    10021 attttctatc catatgaaaa ataatagtaa atctataaat cagttgctat tgatgaatag
    10081 atactaaaaa gccggagcag tctcccgctc cggcttcaca aggcaatcgc ctttcagcaa
    10141 aacacaatta ctctttatcg cccagcagaa cggattccag tgcgattttg atcatgtcgt
    10201 tgaaggtggt ctgacgctca gcggcagtgg tctgctcgtg agtacggatg tggtcagaca
    10261 cggtgcagat ggtcagggct ttcgcgccaa attctgcagc gacgccgtag ataccagccg
    10321 cttccatttc cacgccgagg atgccgtatt tttccatcac gtcgaacatt tcgccgtccg
    10381 gagagtagaa caggtcagcg gagaacaggt taccaacgcg agcatcaatg cccagtgctt
    10441 tagctgcatc tactgcgtta cgcaccatgt caaagtcagc gatagcggca aagtcatggt
    10501 ctttaaaacg gatgcggtta actttggaat cggtgcaggc acccataccg ataacgacgt
    10561 cgcgcagttt tacgtgtggc agaacagcgc cacaggagcc tacgcggata attttcttca
    10621 cgccgaaatc ggtgatcagt tctttggtgt agatggagca ggacgggata cccataccgt
    10681 gacccattac ggaaattttg cggcctttgt aagtaccggt gaagcccagc ataccgcgaa
    10741 cgttattcac ttcacgggca tcttcaagga aagtttcagc aatatacttc gcacgcagcg
    10801 ggtcgcctgg catcaaaact acgtcagcga aatcgcccat ttctgcatta atgtgtgggg
    10861 tagccattgt tttatccttt ttgtgacata acaaaggcgg agtcaacgct ccgcccaaat
    10921 ccatcagaac atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt
    10981 ctggccgata tccgcgaagg tttcacggtg acccagtgaa cccggtttta ctttcgggcc
    11041 gtaaaccagt accggaatgt gttcacgcgt gtggtcagta ccggtccagg tcggatcaca
    11101 gccgtggtca gcagtgagga tcaggatgtc gtcatcgcgc agcagagaca tcagctccgg
    11161 cagacggcgg tcgaacagtt ccagacccgc cgcataacct gcgacgtcac gacggtggcc
    11221 ccaggaagag tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt
    11281 catctctttg atggtggcgt caaacagcgc gtccaggcca gttgctttca cttttttggt
    11341 gataccgcag ttggcgtaga tgtctgcaat tttaccgaca gaaaccacct ggccgtgttt
    11401 ttcatcaacc agtttctgca gcacggtcgg tgctggcggt tcaacagcca gatcgtgacg
    11461 gttaccggta cgctggaagt taccggcttt gtcgccgata aacggacgag cgataacacg
    11521 accgatgttg tagccgccgt tggtcagctc ttcacgggct atttcgcaca gttcgtagag
    11581 tttatccaga ccgaaagttt cttcatggca ggcaatctgg aacacggagt cagcggaggt
    11641 atagaaaatc ggcttgccgg ttttcatgtg ctcttcgcca agctgatcca gaatgaccgt
    11701 accggaagag tggcagttac cgaggtaacc cggcagatta gcgcgttcga ccagtttatc
    11761 cagtagctct tgcgggaagc tgttttcgtg atcggagaag tatccccact caaacagaac
    11821 cgggacaccg gcgatttccc agtgaccaga cggggtatct ttaccggatg acatctcgtg
    11881 cgcccatgcg tacgcaccga taacttcagc gttgccgtcc attcccgccg gaatgaaacc
    11941 ggtagaacct tcgtgtgctt tcgccagccc cagacgggtc agatttggca gattgagcgg
    12001 gcctttacga ccgttatcag cttcgccttt ggcacaagct tctgcgatat gacccagggt
    12061 gtcagccccg acgtcaccaa agcgttctgc atcttctgta gcgccgatgc cgaatgagtc
    12121 cagcaccata ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat
    12181 gatcagatca gtataccgtt attcactgat acgacgatag acagttggtg tgctttccgg
    12241 tgctttatcg gcaagtttaa ttgccgcttt caccgctttc gccgcttcct gccagctgtt
    12301 ttcgtctttc gcgtggataa ccgccagtgg acgctgaccg tctacctggt cgcccaaacg
    12361 cgccatatca gtaaagccga cgctgtaatc gatggtgtca gatgcctgac ggcgtccgcc
    12421 gcccattgca accactgcca tccccagcgc acgggtatcc atttcactga caaaaccttc
    12481 ggtatcagca tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc
    12541 aacgaagtcg gttgggcctt tttgtgccgc taccatacga ccaaagactt ctgccgcttt
    12601 accgttgtcc agcaccgcct gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt
    12661 gccggagata agcatctcta cgcacagcgc catcgtgaca tcaaacagac gcgggttacg
    12721 atactcaccc gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga
    12781 ggccagtacc tgattcatat cggtgagcag cgcggtggta cgcacgcccg cgccgttagc
    12841 tacgccaaca atcgcttcgg caagggcttc agaaagttcg taggtcggca taaatgcacc
    12901 gctacccact ttcacgtcca tcaccagcgc atccagacct tccgccagtt tcttcgccag
    12961 gatcgaggca gtgatcagcg ggatggagtc cacggttgcg gtaatatcac gggtcgcgta
    13021 gaaacgttta tccgccggag ccagtgagct ggtctgaccg ataatcgcca cgccgacgtc
    13081 tttaataatt tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc
    13141 cagtttgtcg agcgtaccgc cagtatgacc gaggccgcga ccggagatca tcggaatata
    13201 gccgccgcag gctgcgacca tcggccccaa catcagcgaa gtcacatcgc cgacgccgcc
    13261 ggtcgagtgt ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt
    13321 tcctgaatct cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa
    13381 gaaaatggtc atcgcgaggg cggcaatctg cccttcggag atagtgttgt cgcgaatacc
    13441 gttgataaag aaacgaattt cttcatcgct cagcgcatga ccatcacgtt ttttacgaat
    13501 aatttcttgt gcgagaaaca aggtaacctc ccgtggaaaa gagacgaggt aatcggggaa
    13561 ccggatgagc atcgctgcgt catccggcaa atcaagcctt agtagctgct ggcgctctta
    13621 ccgtcgccgt ggcccagagc cttcagcagg cttgccagca ggctggaagc gccaaagcgg
    13681 tagtgacgcg catcggccca gtcagcgccg aacagttcat cggcgatggc gagatatttc
    13741 tgcgcatctt ccgcagtacg cacgccgccc gccggtttga aaccaacggt tttttctacg
    13801 cccatatcac ggatcacttc catcatgatg cgcgcgcttt ccggcgtcgc gttcactgcc
    13861 actttaccgg tagaggtttt gatgaagtca gcacccgctt tgatagagat ttcagacgct
    13921 ttgcggatca gcgcttcgtc ttttagttcg ccggtttcga tgatcacttt cagcagtaca
    13981 ttcgctgccg cgcaagcctc tttacaggct ttcaccaggt caaaaccaac ctgctcgtta
    14041 cccgccatca gcgcgcggta cgggaacaca acgtcaactt catcagcacc gtaggcgatt
    14101 gccgcacggg tttctgccag cgcgatttcg atgtcgtcgt taccgtgtgg gaagttggtt
    14161 accgtagcga tacggatttc cggggtaccc tgctctttca gcgttttgcg agcaattggg
    14221 ataaagcgag gatagataca gatagcggcg gtattgccga ccggagtttt ggcctgatga
    14281 cacagggcga tcactttctc gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat
    14341 ttcagtgcac gcaggctgct tgctttcaga tcagtcattt cattctcctg gcttgtcgcc
    14401 aataaaattc accttgcgag tttgttagta ttctaacatc tactccgcaa cacacttcga
    14461 tatacatcac aattaaggaa atctacttac aagtttgcat cactgtaatg cgatctggtt
    14521 caaataattc actttcaaat gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga
    14581 acttcacgaa acttcttaca atggcgcatc accttcggca taaaaggaaa agaagatgcc
    14641 cacttctcat gaaaatgcac tgcaacaacg ttgccagcaa attgtcacca gcccggtgct
    14701 tagcccggag cagaagcgcc attttctggc gctggaagca gaaaacaatc tgccttatcc
    14761 gcagcttcct gccgaagccc gccacgcgct ggatgagggt gtaatctgcg atatgtttga
    14821 aggtcatgcg ccgtacaaac cgcgctatgt cttacccgat tacgcccgtt ttctggcgaa
    14881 cggttccgaa tggctggagc tggaaggcgc gaaagatctt gatgacgcac tctctctgct
    14941 gaccattctt taccaccacg taccgtcggt cacatcgatg ccggtctacc tggggcaact
    15001 ggatgcgttg ttgcaaccgt atgttagaat tctaacacaa gacgagatcg atattcgaat
    15061 aaaacgtttc tggcgttacc tcgacagaac cctgccagac gcctttatgc acgccaatat
    15121 cggcccgtct gattcgccca ttacccgcgc gatcttacgt gcagacgcag agttgaagca
    15181 ggtttcaccg aacctgacct ttatctacga tcctgaaatc acccctgatg acctactgct
    15241 ggaagtggcg aagaacatct gtgaatgtag caaaccgcac atcgccaacg gtccggtgca
    15301 tgataaaatt ttcacaaaag ggggctacgg gattgtgagc tgttacaact cactgccgct
    15361 ggcgggtggt ggcagcacgc tggtacgcct taacctgaaa gccattgccg agcgcagcga
    15421 atcgctggaa gacttcttta cgcgcactct accgcactac tgccagcagc agatcgccat
    15481 catcgatgcg cggtgtgaat tcctctatca acaatcacac ttctttgaga atagcttcct
    15541 ggtgaaagaa gggctgatta accctgaacg ttttgtgcca atgtttggca tgtatgggct
    15601 ggcggaagcg gttaacttgc tgtgtgaaaa agaggggatt gccgcgcgct acggtaaaga
    15661 ggccgccgca aatgaagtgg gttatcgcat cagcgcgcaa ctggcggagt ttgtcgccaa
    15721 tacccccgtg aaatatggct ggcaaaaacg cgccatgtta cacgcacagt cggggataag
    15781 ttccgatatc ggcaccacgc cgggcgcacg tttgccgtat ggtgatgagc cagatccgat
    15841 cacccatctg caaaccgtcg ccccgcatca tgcttattat tattccggca tcagcgacat
    15901 tctgacgctc gacgaaacca tcaaacgtaa tccgcaggcg ctggtacagc tttgcctcgg
    15961 tgcctttaaa gccggaatgc gtgaatttac cgccaatatc agcggtaacg acctggttcg
    16021 cgttactggt tatatggtgc gtttgtcgga tctggaaaaa tatcgtgccg aaggctcacg
    16081 taccaacacc acctggctgg gagaagaagc cgcacgtaac actcgtattc tggaacgcca
    16141 gccgcgcgtg ataagccatg aacagcagat gcgctttagt cagtaagatt atccccttct
    16201 cctgcgttga cgggccaggc agtcgtctgg ctctgttttt gcagggctgc aatctgcgct
    16261 gcaaaaactg tcacaatccg tggacgatgg gacgttgcaa tgattgcggg gagtgcgtgc
    16321 cacagtgtcc gcatcaggcg ttgcagattg ttgacggcaa agtggtgtgg aacgctgtgg
    16381 tctgcgagca atgtgatacc tgcctgaaga tgtgtccgca acatgccacg ccaatggcgc
    16441 agtccatgag cgtggatgag gtgcttagcc atatccgcaa agcagtgctg tttatcgaag
    16501 ggataacggt aagcggcggc gaagccacga cccagctacc gtttgtggtg gcgctgttta
    16561 ctgctatcaa aaatgatccg caactgtgcc atctcacctg tctggtggac agtaacggta
    16621 tgttgagcga aaccggctgg gaaaaattgc tcccggtgtg cgacggcgcg atgctcgatc
    16681 tcaaagcgtg gggtagcgaa tgtcatcaac agttaaccgg acgcgataat cagcagatta
    16741 agcgcagcat ctatttgctg gcagagcgcg gcaagctggc ggaactgcgt ttgctggtaa
    16801 tccctggcca ggtggattat ttgcaacaca tcgaagaact ggcggcgttt atcaagggac
    16861 ttggcgatgt tccggtacgc ctgaacgcgt ttcatgccca cggcgtgtat ggcgaggccc
    16921 aaagctgggc gagcgccacg ccggaagacg ttgagccgtt ggctgatgcg ttaaaggtgc
    16981 gcggggtgag ccggttgata tttccggcgc tgtatttgtg acatgccgga tgtggcctac
    17041 agttcggcac agacttgtag gcatgataag acgcgtcaag cgtcgcatca ggcattgtgc
    17101 gccaactgcc ggatgcggcg tgaacgcctt atccggccta cagcacgtta aacaacgtat
    17161 acgtgttatt aagcaacact tcggcaattt catctgcggg ttccaagcgt aactcacaaa
    17221 gcacagcaaa cacccgcgcg gcctgttccg ggcggttagg ctgcccctga aaaccgttga
    17281 gcggcatatc cggcgcgtcg gtttccagca acaacgatgc cagcggtaat tttgcgatga
    17341 catcgcgggt tttactggcg cgtggatagg tgatagtgcc gcctacgcca attttgtagc
    17401 ccagctgtac aaaccgttca gcctgttgca ggctgccgga aaaaccgtga accacgccag
    17461 tacgcggcaa atcgtggcgt ttaagatgca tcgccagttt gtcgtgcgtg cgccgtgaat
    17521 gcaggatcac cggcagatcg tagcgtttcg ccagtttcag ttgttcgtcg agtaaccact
    17581 gctgcctctc aaattgcgga tcgtcgccaa agagatccag gccgatctcc cccaccgcca
    17641 ccaccttcgc cggatgcctt tccagcgcct gctgtaactg ctccagcgat gcatcgctat
    17701 gtttttctaa catccccgga tgcaacccta acgcgccata cagcggttga tatttttccg
    17761 ctaacgcctg cacgcgggca aaattttccg cctctgtcgc cggaacaatg atcttgccta
    17821 cgcccgcttg tgctgcgcgt tgcaggctgg cctcttcatc gccgctaaac ggcgggaaat
    17881 caaaatggca gtgggtatcg ataaaacggc aaatcaagcc agatcctcat tgttaaatgt
    17941 cgggtcgttc gcctgcggag cgtcgctcac ttcggcaacc agcgtatcgt tggcgactgg
    18001 cgcaggcgga atgacaatcg attccggcgt caccactggc accagatggc gggttagcgg
    18061 cgctttttca gttaatagct tgccaaccgt tgccaggaaa taacgaccgc ataaacgccc
    18121 aagtttatag tcttcacgca gcgccggaat ccgactgcca agcgcgatac tatgcaatgg
    18181 cttcggcgga taaatttcga atatccgcag tttgcccggt ggtttctcaa taaattgctg
    18241 aatgtcacga tagctggttt catgatgctg caccagattg accagcggct gcaggctact
    18301 gtcacccagc cagcgttcca tgcgtttgaa ccactgcggc gtgtagtaca tttgcgacgg
    18361 cacggtgcgg atgacgacca aggtttttgc tccctgcctt gccgcctctt taaccggaat
    18421 cgcatcactg atcccgccat ccaggtagtt aatgccttcc agcgacactc cgctacgata
    18481 aaagccaggt atcgcactgg aggcgcgaat cacatccagc cagttttgtt tggttggtaa
    18541 aaagtaattc ggcgcatagt catcctgacg acaggcgcac atataaaacg atttgccgct
    18601 gtcaaacaac cgcgcggcgg tatccatttg caacggcatc tggcttgcag tggcctccac
    18661 cagccagtcg agatcgataa gatttcctcc acggacaaag cgcaatggat cgaaaaattc
    18721 gcgttttgtg gtatagcgca tgatgacttt gcgcgcgtaa ccgggctgat tgcagatata
    18781 cgccgagagg ttctgcgccc cggcagatgt gccgagataa agatcgaaag gattaaactg
    18841 cgcgcgcata aactcatcca gcacgccagc cgtgaaaatt ccacgctgtc cgccgccttc
    18901 gcacaccaga gctattcgtc caggctggaa cggccttagc gacaacggcg caatattacc
    18961 aagcgttaca ggtattcgct gccccacctc tgccttcctg tttttgttgt gaaagaatga
    19021 caaagtaacg caatttatta ttcgctaaaa ccacaaaagc cagtccgctg gactggcttt
    19081 ggataaaatt ttcttaatgg tctgaaatct agggtcgttt tcggcccatg aacaaactca
    19141 ccaggaacag aataatcccg acgacaaaga caattttagc tgcgcctgca gcggtaccgg
    19201 ccagaccacc aaacccaagt gcggcggcga ttaacgcgat aaccagaaat atgatgcccc
    19261 aacgaaacat aagcgtctcc tttaccatag ttaatgtcac cgcttgatat gagcgtgaaa
    19321 atcacacaat gcgatatttt tagtgtggtg cacatcgcgc ctcccgacaa agttcgggag
    19381 gacgaattac gacgaattac ttagttttga gatcattttt aacgcttttc acaccatcta
    19441 ccgctttggc gatactttca gcacggtcgc tctgtgcctg ggaatcgacg gtaccggaga
    19501 gctgaaccac gccgtcggtg gtttcaactt tcacatgacg ggaagggacg atatcgtccg
    19561 ccagcagttt ggctttgatt tcactggtgg tggcggtgtc acccgcgtag cccttcaccg
    19621 agccttcttt agcgtcgcga acgtgcagtt tgtcgctgac agaggtcacc ccttcaacgc
    19681 ctttcgccac tttcactgcc tcttcggcct gagcctggct ttcaacgaaa ccgctcaggg
    19741 tcacgacttt ttgatcggtt ttgacagaga tatcggtgct cttaatgttg tcatgatcca
    19801 ccagcgccgc cttcactttc gcggtgatgg cgctgtcatc catgaaatta ccgactttat
    19861 tcatagagct atcgactttt tgccctgcgc tttcattggt agtctgcgcg ttgttttctg
    19921 cgtaggcaga gccggtcgcg acggcagagg tcaacattac agccagcaga gttttcgaaa
    19981 tcttcagtct tgtcatagtc atcgatttat tcctgtatgt ttgctcgtaa tttgagcctg
    20041 gcaacacgag gttgcattgc tgaataggga gagacttcac cctctacaga agtcaatggt
    20101 cgccatcaca aaagcgatgg gtgatgaata acgaccatta cagcctctga atcagttatt
    20161 aatatcggta gaatgacaat cgacggcttt agatactgat atctacgcat tgaacggtat
    20221 ttaacgccgt cagaaatgtc attactttgt taaatataga tcacaatttt gaaaccgctc
    20281 gggatatcag cgaaaacata agcaaaagtg aatgttttaa gaacattccg taagcggcta
    20341 ataaggaagg gaaattgaca gggcgcagcg gctaccgcgc cctggaggca agaattaatg
    20401 ctcgcgggtc tggtggaact gaacgtccgg ataacgttcc tgcgccaggc gcaggttgac
    20461 catgctggta gcgatgtaag cgaggttatc gccgccatca agcgccagtt ggctttcgtt
    20521 cttacgcttg aactcttcga atttcttcgc gtccgcacat tctacccagc gggcagtggc
    20581 aacgttgact gactcataca ctgcttcaac gttgtattcg ctcttcagac gcgctaccac
    20641 cacatcaaac tgcagcacac caaccgcacc aacgatcaga tcgttgttgg agatcggacg
    20701 gaacacctgc accgcaccct cttcggaaag ctgtaccagc cctttgagca gctgtttttg
    20761 cttcagcgga tctttcaggc ggatacgacg gaacagttcc ggcgcgaagt tcggaatacc
    20821 ggtgaacttc atcatctcac cctgggtaaa ggtgtcaccg atctgaatag tgccgtggtt
    20881 gtgcaggccg aggatatcgc ccggatacgc ttcttcaacg tgcgaacggt cgcccgccat
    20941 aaaggtcagc gcgtcggaga tcaccacatc tttcgcggtg cgcacctggc gcagcttcat
    21001 gcctttttca tatttaccgg ataccacgcg cataaacgcc acgcggtcgc ggtgtttcgg
    21061 gtccatgttg gcctgaattt taaatacgaa gccggtaaat ttgtcttcgc tcgcttctac
    21121 agtacgggta tcggtctgac gcggcatcgg cgcaggtgcc cactccacca agccatccaa
    21181 catatgatcg acgccgaagt tacctagcgc agtaccgaag aataccggag tgatttcgcc
    21241 cgcaaggaat aattctttgt cgaactcgtt agacgcgcct ttcaccagtt ccagttcgtc
    21301 acgcagctgc tgcgccagat cttcaccaac cgcagcatcg agatccgggt tattcagccc
    21361 tttaacaatg cggacttcct gaatcgtgtg gcctttaccg ctctgataga gataggtttc
    21421 atctttataa aggtggtaaa cgcctttaaa cagcttgccg cagccaatcg gccaggtgat
    21481 cggtgcgcag ccgattttca gctcgttctc aacttcatcg agcagctcca tcgggtcgcg
    21541 gatatcacgg tcaagtttgt tcataaaggt gaggatcggc gtgtcgcgca gacgggtaac
    21601 ttccatcagc ttacgggtac gatcttcaac accttttgca gcgtcgataa ccatcaggca
    21661 gcagtccacc gccgtcaggg tacgataggt atcttccgag aagtcttcgt gccccggggt
    21721 gtcgagcagg ttaaccaggc agtcgtgata cggaaactgc atcacagacg tggtaatgga
    21781 gatcccacgc tgcttttcca tctccatcca gtccgactta gcgtgctggt tagagccacg
    21841 gccttttact gtaccggcgg tctgaatggc ctgtccgaac agcagcacct tctcggtgat
    21901 ggttgtttta ccggcgtccg ggtgagaaat aatggcaaaa gtgcggcgct tcgccacctc
    21961 ttgcaaataa ggagacaacg tcataattct ttcttcttga gtaagcggca tcgcgccgcg
    22021 cttgttgaaa acgaaaaatt gcggctattt tacccatcaa cggggggggg aggcaatcag
    22081 tgtttacaca ggagctgctc cagttcgtgc aacgaagaaa cggtccaggt gggcgcgatg
    22141 ccttctggtt gctcgcgatt gtgcgcattc agccagcagg tcgcaagccc ggcgttgatg
    22201 ccaccgagaa tatcggactc ggcagtgtcg ccaaccatca gcacgcgtga acggtcagga
    22261 ttgcccgcct gttccagcgc ataatcgaaa attttcttat tcggtttggc aacgccaact
    22321 tcttcggaaa tcaccagcaa atcgaaataa tcacgcaggc ccgtgcgttc cagacgcact
    22381 tgctgcaagg cgctaaagcc gttggtgatg atgccaattt tagcgttgcc acgaatggcg
    22441 ttaagcagag aaaccgcgcc cggcagcggc gtacagattt ccgccatcgc attaataaag
    22501 gcttcgttga gtttacccgg ctcgacgttc agccgttcgg cccagctctc aaaccgcccg
    22561 tgctgaagct gtaatgaagt gatcgcgccg ttttgataat ccacccacag tggcttgtta
    22621 acggcctgat agtcctgaaa atcttcagcg gtaaaggtga cgctgtaatc aagaaacatc
    22681 cgctgcaggc cggtgaatga gtcaaaggta aacagcgttt catcggcatc aaagaaaatc
    22741 cagtcccact tcattattcc accttgtctt acatactgat tggcaacgcc atgatgatgg
    22801 cgtcttcgcg accgtccgtg gtggggtagt aattgcggcg aattgtcgcc tcgttaaagc
    22861 ctaaactttc gtacagggca atggcggcag cgtttgaagc acggacttcc agccatagtg
    22921 tcgccacgcc gcgtttttcc agttcgtcga tcagatgttc cagcagcgcc cttcccaatc
    22981 cctgacgctg atagtcagga tcgaccgcaa tattgaacaa tgtagcttca tccagcacca
    23041 cttgcgtaat cgcaaacgcc gccattttgc cgttttgcgt taactgaaag ttgagataac
    23101 gctcgccctg gttgctggca aaggtttttt cactccacgg aaaggcgtgg gcgcgttgtt
    23161 caatatggta agccgctggt aaatcagccg tttcgaggga agaaatcgtg ttcatatgtg
    23221 caaatttgtt gccataacgc ggcgcgtgcc gttgggtttg cccgtaattc ggtgagcgcc
    23281 ggtgattcca cctgagcgcc ttccaatgat agcggttcgt cagtacccaa ccgccagctg
    23341 ttgcagcgac tgccttgcgg cagcatcgcg attttttctg gcgtcagttg cagcacttga
    23401 tcggggctga cggttaatgc gcgcagaaca tcgctcacta aaggatcagt cagggcggga
    23461 agatcgtttg ccaccatcac cagacggacg tgtgccggga tcgcaatggc aatctccccc
    23521 tgcaacgcgc cagggcgacg cagcgaccac tgggtaatgc ccagttgctg taactgccag
    23581 tctcgtcggg atgtcatagc gaatcgctcc tgttgtcagg ggcgcaaata tagcaaattc
    23641 gtcgataccg cgccaacata tggctataat cgccgccagt atcaattgag gagcattcca
    23701 tgtctgcatt taccccggca agtgaagtct tgctgcgtca cagtgatgat ttcgaacaaa
    23761 gccgtattct gtttgccgga gacttacagg atgacctgcc cgcgcgttta gataccgcgg
    23821 ccagccgtgc tcatacccag caattccacc actggcaggt attaagccgc cagatggggg
    23881 ataacgcccg tttcagtctg gtcgccacgg cggatgacgt cgcagattgc gatacgctga
    23941 tttactactg gccgaagaac aaaccggaag cccagttcca gttgatgaat ttactttctc
    24001 tgctgccggt ggggacggat atttttgtcg ttggcgagaa ccgcagcggc gtgcgcagcg
    24061 ccgagcagat gctggcagat tatgcgccgt tgaataaagt cgacagcgct cgtcgctgtg
    24121 gcctctattt tggtcgtctg gaaaaacagc cagtatttga tgcggataaa ttctggggcg
    24181 aatacagcgt cgatggcctg acggtcaaaa cgctgcctgg cgtgtttagc cgcgacggtc
    24241 tggatgtcgg tagccagttg ttactctcga cgttaacccc gcacacgaaa ggtaaagtgc
    24301 tggatgtcgg ctgtggcgcg ggggtgcttt cagttgcctt tgcgcgccat tcgccgaaaa
    24361 ttcgtctcac cttgtgcgat gtctctgcgc cagcggtaga agccagccgc gcaacacttg
    24421 cggccaactg tgttgaaggt gaagtctttg ccagcaacgt cttttccgag gtgaaaggtc
    24481 gttttgatat gatcatctcc aacccgccgt tccacgatgg gatgcaaacc agcctggatg
    24541 cggcgcaaac gctgattcgc ggcgcggtgc gtcatcttaa tagcggcggc gagctgcgaa
    24601 ttgtagcgaa cgccttcctg ccttacccgg acgtgctgga tgagacattt ggcttccatg
    24661 aagtgattgc gcaaaccggg cgtttcaagg tgtatcgcgc cattatgacc cgccaggcga
    24721 agaaaggtta attatctcgc cgaataccgt gtcggatgcg gcgtgaacgc cttatccgac
    24781 ctacaaaacc tgcacgttag cccttcgtag gccagataag acgcgccagc gtcgcatctg
    24841 gcatctgccc ccgcatgcct accaaacctg cacgctagtt tcctgatgga catttttcca
    24901 gcaattacac ctccggcgat aattaactat tgacgaaaag ctgaaaacca ctagaatgcg
    24961 cctccgtggt agcaattctt tttaagaatt gatggtatgc gaaggtggcg gaattggtag
    25021 acgcgctagc ttcaggtgtt agtgtcctta cggacgtggg ggttcaagtc ccccccctcg
    25081 caccaaaaac cacgttgata ttgctcgcac tgggcgaagg tggcggaatt ggtagacgcg
    25141 ctagcttcag gtgttagtgt tcttacggac gtgggggttc aagtcccccc cctcgcacca
    25201 aacgaggcga tatcaaaaaa agtaagatga ctgtgcgaag gtggcggaat tggtagacgc
    25261 gctagcttca ggtgttagtg tccttacgga cgtgggggtt caagtccccc ccctcgcacc
    25321 aattatcttt acttcctttc ttgtttcttc cttgattttt catccctgat tcaaattcat
    25381 tatcatcagc atgatggcag caataagcgc caggcacgat ggtagcaaaa cgtaatgccg
    25441 taactgtttg tgatacagca tcaccgacgt caatactaat cccatcagaa tgacaatccg
    25501 ccactgatcc ggtgataaat ccgcatacat cagccctgca atcagcaatc ctggcagcaa
    25561 cactccccag ccactgccca gcgtacgttg caacatggtt tcatctcttt cattgataat
    25621 gataaccaat atcatatgat aatttttatc atttgcaagc cagagaaatc ccttgctatc
    25681 ggataaacct atcgctagga ttagcaatca ttatcattta gattactatc ccgattatgg
    25741 cctatcgttc cgcaccgctc tatgaagatg ttatctggcg aacgcatctc cagccacagg
    25801 atgctgggct tgcacaagcc gtacgtgcga cgatcgccga acatcgtgaa catttgctgg
    25861 agtttatccg cctggatgaa cccgctccgc ttaacgccat gacgctggcg caatggtcat
    25921 caccgaatgc gctaagttct ctactcgcgg tctattccga tcatatctat cgcaaccaac
    25981 caacgatgat ccgcgagaac aaaccgctga tctcgctatg ggcacaatgg tatatcggcc
    26041 tgatggtgcc accattaatg ctggcgctac tgacgcagga aaaggcatta gatgtgtcgc
    26101 cggaacattt ccatgctgag tttcacgaaa ccggccgtgc cgcctgtttc tgggtcgatg
    26161 tgtgtgaaga taaaaacgca acaccacatt cgccgcagca gcgaatggaa acgttaatca
    26221 gccaggcgct ggttccggtt gtgcaggcac tagaagcgac cggagagatc aacggcaaac
    26281 ttatctggag taataccggt tatttgatca actggtatct cactgagatg aagcaactgc
    26341 tcggcgaggc gacggttgaa tcgctgcgcc atgccctctt ttttgagaaa acgctttcta
    26401 ccggtgaaga taatccactc tggcgtaccg tggtactgcg cgacggcctg ctggtgcgcc
    26461 gcacttgctg ccagcgttat cgcttaccgg atgtgcagca atgtggcgat tgtacgctga
    26521 aatgaaagca ctgccgggga agtaaacccg gcataatgcg gattaatagg gatgcaacac
    26581 attacttgct tcgcaatgcc gcatacataa cagaatatct gcggcctcca acaaccctgc
    26641 atcagaactg acgcccagtt tcgacatcac attaaattta tgtgcacgga tcgttttgat
    26701 attgcgttta agctgctcgg caatttgtgt cattgagtag ccacgcgaca taaagcgcaa
    26761 tatttctctc tccgtcgggc ttaacgtccg gctttggtta atgtaccact gattgttcag
    26821 tcggtcggtc gcctgacgta caccatttaa tgacaaaaag agttcctgat gaaaaatctc
    26881 cagcgttgac gctttactta atacaccgtc cagcggtgat ggcgacaatg aaccaatcag
    26941 ccgagcttca atatcatcat ccgcaataac taaacgccgg gtacgcggaa acttaatcgc
    27001 cagttcagtc aggcaagata atccctctct gcgctcactt ctcatggcag aaaaagaaaa
    27061 aatgaccgcc gcaaatgaga tccgcgacat ggcatcctga aatgactcct gactggtata
    27121 ggtatggagc ttataagggt tacccgcaaa ctttctgaat aaactctcaa taccgatact
    27181 gctcatgacg catttttcta tgagtgcgac atttctcttc ttaattcggc tgtgttccat
    27241 tctgcggcat ctccataaac agaaggcaag ttaagttctc ttgcaccctg cgcgctattt
    27301 atccatgcat acatttcagc gttgctgtga attgccagtc gacgcatcgc actgtttttt
    27361 tgggcgctga tagttttatt acttttctta agcaacgaag ctatctggtt gatgccccat
    27421 cctttaccta atagccgcag taccttgcgc tctgaaagag tgagaatgac ggagcgatag
    27481 ctaaaatcat gagtttcagt cattgccgga gaggtcagca tggcgctgat acgctctgcg
    27541 tgcgtattgc cggaacgtac ggtcttgacc agattctcaa tgggttcaac atcagacaat
    27601 aacgtcgccg tagggcacat gagcaattct actgcctggg aataccagga gcgtgacacc
    27661 atgaaaaccc agtgaatttc ccgatattgt gagattaaag aataataatg ttcacaaaca
    27721 gaacgtggat cttcactttc accggctaaa tcagcaatga ctaatccgga acgacgtaat
    27781 tgtaataagg taaggtcctc cgcagaggcg ctggatatta tttcatattc agggaagtga
    27841 gtcctcatga cctcttttaa ccctgcttgc ataacaggta ttttactgat aacaattcca
    27901 tttttgcagc atcctggcaa catatttcct ccacatccac tgtctaaaaa tcatgatggt
    27961 ttattcagga tattcattcc ttaaatgttt ccagcaaatt aatagattaa gcgaataaat
    28021 tatccttagc attcactttc atatcagcat actcctgctg cacaacaact ccactgcatg
    28081 tgtcagaagg ttagcatcaa atgataacct ggtgaaatag cctggagaca tttaaaatat
    28141 ctacattaag ttgttaggaa tacttaatga aaaacagaaa tcacattcat ttaatttata
    28201 attaaatgat atcccttccc gcagagtgcg ggaatcacac aatcaatatt tattcaataa
    28261 aatctgatgg ttttaacttg ttaacataaa aaaacgcgcc cagctatcta ttatcctgac
    28321 tcgtaaaggc caaataacaa attcattata gtgataatat tcatgcgata gtttgtttac
    28381 ggcgagagat aattcgcttt ttatcaccga gcatctcccg ataaccagga ttaatcagcg
    28441 caattttgat cttaacctac cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat
    28501 tatctgaaca atacgcagca cgtctatgac aaagggcgag ttatgcaaac tgagcaacag
    28561 cgagccgtaa cacggctttg tatccagtgt ggattatttc ttttgcaaca tggtgcggaa
    28621 agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg cgttgggaat ggacagcgtc
    28681 gaaagttcta tctcttcgaa cgccatagtg ctgacaacca ttaaagatgg gcaatgcctg
    28741 acatcgacac gtaaaaatca cgatcgcggc attaatatgc atgtggtgac tgaagtccag
    28801 cacattgtaa ttctggcgga gcatcatctg ctggattaca aaggcgtaga gaaacgattt
    28861 agccaaattc agccattacg ttacccaaga tggctggtag ccttaatggt tggcctttcc
    28921 tgcgcctgtt tctgtaaact caataaaggt ggctgggatg gtgccgtcat caccttcctg
    28981 gccagtacgg ccgcgatgta tatccgccag ctgctggcac aacgtcatct tcatccacag
    29041 atcaactttt gcctcaccgc tttcgccgcc accaccattt ccggattgct tttgcaactt
    29101 cccactttca gcaatacccc caccattgcg atggccgcca gcgttctgct gctggtgccg
    29161 ggctttccgt tgataaatgc cgtcgccgat atgtttaaag gccacatcaa taccggactg
    29221 gcacgctggg caatcgccag tctgctgaca ctggctacct gtgtcggcgt agtaatggca
    29281 ctgacgattt gggggctacg cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg
    29341 atatgatcct cgccgccatt cctgcggtcg gctttgcgat ggtgttcaac gttcccgttc
    29401 gagcgttacg ctggtgtgcg ctgcttggtg cgataggtca tggttcccga atgatcttaa
    29461 tgaccagcgg gctgaatatt gaatggtcaa cctttatggc ctctatgctg gtcggcacca
    29521 ttggtattca atggtcgcgc tggtatctgg cgcatccgaa agtgtttacc gtggcggctg
    29581 ttatccctat gttccctggc atatcagctt ataccgcaat gatttcggcg gtaaaaatca
    29641 gccagctagg ttacagcgaa ccgttgatga ttaccctgtt aaccaacttt cttacagctt
    29701 catcgattgt tggtgcgtta tccatcggtc tttccattcc tggattatgg ttgtaccgca
    29761 agcgccctcg cgtataaaat tgcctcactt aaagcacgcg gatgagagac agcctcctct
    29821 cctccgtgtg ttactataaa agttatctcc cttctcgttc atcgttccat atttgagaaa
    29881 cagtatgtct tccagagttt tgaccccgga cgtcgttggt attgacgccc tggtacacga
    29941 tcaccaaacc gttctggcaa aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc
    30001 cccggcgttt tatgccatca ctcctgcacg cctggctgaa ctgctggcgc tggaagaaaa
    30061 gctggcgcgt ccgggaagcg atgtcgctct ggacgatcaa ctctatcagg aaccgcaaac
    30121 cgctcccgtt gccgtaccca tggggaaatt tgccatgtac ccagactggc aacccgatgc
    30181 cgactttatc cgcctggcgg cgctatgggg cgtggcacta agagagtcgg tgaccgccga
    30241 agaactggcg tcattcattg cctactggca ggcggaaggc aaagtctttc accatgtgca
    30301 gtggcaacaa aaactggcgc gcagcctgca aatcggtcgt gccagcaacg gcggactgcc
    30361 gaaacgagat gtgaatacgg tcagtgaacc tgacagccaa attccaccag gattcagagg
    30421 gtaacgatga aaaacgttgg cgacctgatg caacgcctgc aaaaaatgat gcctgcccat
    30481 atcaaacctg ctttcaaaac gggtgaagaa ttactggcct ggcagaaaga acaaggggcg
    30541 atccgctccg ccgctctcga acgtgaaaat cgggcgatga aaatgcagcg cacctttaac
    30601 cgctccggta ttcgtccact gcatcagaac tgctcctttg agaactatcg cgttgagtgt
    30661 gaagggcaaa tgaatgcgtt aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac
    30721 atcgccagct tcatcttttc tggcaagcca ggaaccggca aaaaccatct ggcggcggca
    30781 atctgcaacg agctgctgct acgcggtaaa tccgtattga tcatcaccgt ggccgatatt
    30841 atgtcggcga tgaaagatac cttcaggaat agcggtacca gcgaagaaca actgcttaac
    30901 gatctgagta acgttgatct gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa
    30961 tacgaaaaag tgatcatcaa ccagatcgtc gatcgccgct cttcttccaa acgcccaacc
    31021 gggatgctga ccaacagtaa tatggaagag atgaccaagt tgctgggcga acgcgtgatg
    31081 gaccgtatgc gcctgggtaa cagtttgtgg gtgatcttca actgggatag ctaccgtagc
    31141 cgtgtaacag gtaaagagta ttaatccccg tttatggcgg tatactgcga cacaattcaa
    31201 ccttgagtaa tgatgatgaa aactattaaa catcttctgt gctgtgccat tgccgccagc
    31261 gcattaattt ccaccggggt gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc
    31321 agcgaacttg gcaaccaaaa caacacgaca caggaaggcg gttggtcgct cgcgtcatta
    31381 actaacttgc ttagcaacgg gaaccaggcc ttaagcgcag ataacatgaa caacgccgca
    31441 ggcattctgc aatactgcgc gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc
    31501 aagaatcagg tactggaaaa actgggcctg aacagtgaag agcaaaaaga agacaccaac
    31561 tatctggatg gcattcaggg tttgctgaaa accaaagatg gtcagcaact caatctggat
    31621 aatattggaa cgactccgct ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta
    31681 aagcaggggc tgaacttcat ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg
    31741 ctgacgcggc tctctcctgc ccttttccct gctcctggac ggttttaccc ctgttacacg
    31801 ggaagtgacc gcgatcaaaa aagtctgttt ataaactaat tcctaacaac atcgcatttt
    31861 tttgacacta gaattgcagc aatatggcga cgccattaca ttgtgcttct caaaaagctt
    31921 aaacgttggc taaacggccc ggtttagtga ggatcgcccg ttgtcagaac tactctcttt
    31981 cgcccttttt ctcgcctctg tgctgattta cgcatggaaa gcgggacgta acacctggtg
    32041 gtttgcagcc acattaacgg tactggggct atttgtcgtt ttaaatatca ccctgtttgc
    32101 cagcgactat tttactggcg atggtattaa cgacgcggtt ctctatacct taaccaacag
    32161 cctgaccggt gctggtgtca gcaaatacat cctgcctggt atcggcattg tgctggggct
    32221 ggcggcggtg ttcggtgcgc tgggctggat cctgcgccgt cgtcgtcatc atccgcacca
    32281 ttttggttac agcctgctgg cactcttact ggcgctgggt tcagtggatg ccagcccggc
    32341 atttcgtcag ataacggaac tggtgaaatc ccagtcacgc gacggcgacc cggactttgc
    32401 ggcttattat aaagagccgt cgaaaactat ccctgacccg aaactcaacc tggtttatat
    32461 ctacggcgaa agtctcgagc ggacctattt tgataacgag gctttcccgg atctcacgcc
    32521 tgaactgggc gcgttgaaaa atgaaggcct ggatttcagc cacacgcagc agctgccagg
    32581 aacggattac acgattgcgg gcatggtggc ttctcagtgc ggcataccgc tgtttgcccc
    32641 atttgaaggc aacgcctccg cttctgtttc cagcttcttc ccgcagaaca tctgtctggg
    32701 cgatattatg aaaaactcgg gttatcagaa ctatttcgtg cagggcgcga atctgcgttt
    32761 tgccggtaaa gatgtgttcc tgaaatcgca cggcttcgac cacttatacg gctcagaaga
    32821 gctgaaaagc gtggtggccg acccgcacta tcgcaacgac tggggattct acgacgatac
    32881 cgttctcgat gaagcgtgga aaaaatttga agagctttcc cgctcaggtc agcgattctc
    32941 actgtttacc ctgacagtcg atacccatca cccggatggt tttatctcgc gcacctgtaa
    33001 ccgcaaaaaa tatgattttg acggtaagcc gaatcagtca ttcagcgcgg taagttgcag
    33061 ccaggagaat attgcgacgt ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac
    33121 cgtcatcgtc gtctcttctg accatttagc gatgaacaat acggcgtgga aataccttaa
    33181 taagcaggat cgcaataacc tgttttttgt catccgtggc gacaagccgc agcaagagac
    33241 gctggcagtg aagcgtaata cgatggataa cggcgcgacg gtgctggaca ttctcggtgg
    33301 cgataactat ctcggacttg gccgtagcag tttatccggg cagtcgatgt cggaaatctt
    33361 cctcaatatc aaagagaaaa cattggcgtg gaagccggat atcatccgcc tgtggaaatt
    33421 ccctaaagag atgaaagagt tcaccatcga ccagcagaaa aacatgattg ccttctcagg
    33481 tagccatttc cgtttgccgc tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc
    33541 ggaaagcgaa tactcagcac cgctgcgttt ccagctggca gatttcgctc cacgcgacaa
    33601 tttcgtctgg gttgaccgtt gctacaagat ggcacaactc tgggctctgg aactggcact
    33661 ctccaccgac tggtgtgtct cgcaagggca gcttggcggt cagcaaattg tccagcatgt
    33721 tgataaaaca atgtggaagg gcaaaacggc atttaaagat acggtcatcg acatggcgcg
    33781 ttacaaaagc aatgtcgata cgctgaaaat tgttgataac gatattcgct acaaagccga
    33841 cagtttcatc ttcaacgtcg ccggtgcgcc agaagaggtg aaacagttta gcgggatttc
    33901 ccgtccggag tcgtggggcc gctggtccaa cgctcagctg ggcgatgaag taaaaatcga
    33961 gtacaagcat ccgctgccga agaaatttga cctggtgatt accgccaaag catacggcaa
    34021 taacgccagc cgtcctattc cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg
    34081 caatgaagta accaccacca cactgcattt cgataaccca accgatgccg acacactggt
    34141 aattgtgccg ccggaacctg tctcaaccaa cgaagggaat atcctcggac actcgccgcg
    34201 taagctcggg atcggcatgg tggaaattaa agttgtggaa cgtgaagggt aattcctgac
    34261 tgatgtgtag gtcgcataag gtgcgaccta cacaccttct ccggcctaca aaacatcatt
    34321 gagaaacaga aaaaaacagc gcccaacact tccccggacg ctgcatggca actttaataa
    34381 tctcgcctta tcagaaagta attacaccct taatcaactc acgattgtta atcacatcgc
    34441 gctcgtaggt ttctgccagc gtggcgaacg gatagcgatg ggttaacatc atgtcggcag
    34501 tgattttccc ttccgccatc aatcgaccga ctttagcgaa atcttccggc gtggcgttgc
    34561 ggctgcccat catcgtcgtt tcttttttat ggaattccgg atcggagaac tgcaactcac
    34621 ctttaaacag gccgacaaat accaccgtgc cgccgtgacg aatcagattt acggtgttat
    34681 tcatcgcatg ttgattacct gtcgcgtcaa tcactttctg cgccagcgaa ccaccaaact
    34741 gcgcccgcag ctgcgcgtca aaatcttcgg ctgacgggtc cagaacaggt aattccagac
    34801 gcgttgccac atgttcacga cgcgccggac tggtatccgc caccaccacc tgcgcgccat
    34861 cggctttagc gattgccgcc gcgcccagac cgattggccc cgccccgacc accagcacct
    34921 gctcgctggg tgcaatggcg gcacgacgca ccgcatgagc gctaatagcg aaaggttcaa
    34981 tcaatgctgc cgcctgcggg tcaataccgt ctgcgggcaa aatgttcgcc accggcacgc
    35041 tcaaatactc actaaaaccg ccatcctgat gcacgccaat gactgaaatt ttttcacagc
    35101 aattggtacg accgctttta cacgccgggc attgctgaca agaaatatac ggaattaccg
    35161 caacttgctg tccgatatta aaattgtgaa tgttttttcc tgattcaata atctccccac
    35221 atatttcatg gcctaaaacg cgtggataac taaaaaatgg ctgattgccc gcccaggcat
    35281 gaatatctgt accgcaaatc ccgacagttt taattttaat taatgcttca ttatcgcctg
    35341 gcaccggtct ttcccggact tcccatacta aattctttat ttcctgacaa attaatactt
    35401 tcattgtgga catgattcac cgctcctttt attttatgac tttcagatat cgtcgaattt
    35461 ataagagcgc ctgaatttac ccataaaata tgcggatgat cgcgcataaa aatgttttaa
    35521 atcggctttt aatgccggac tggagggaag aatgagtcgt tcgcagaatt tacgtcatag
    35581 cgtcataaat caggtgattg aagatatggc gaggggcaat attccttctc cgctgccttc
    35641 acaaagcgga ctggcagaaa tgtataacat cagtcgcact acggtacgcc acatactcca
    35701 gcatttaagc gcatgtggcg tgctcaccct ggtggggaaa aattatgtta tcgcccgcaa
    35761 acccgaacag aacgatggat ttgattgcat cacggcctcg ctgaaagagc aaaaccgtct
    35821 tttcgagcag gcatttttca ccatgattaa cctgcgccag ttacgcgccg gagaaagctt
    35881 ttccgaactt cagctggcgc gagcggcaag cgtcagtccg gtagtggtac gagaatatct
    35941 tttgaaattc ggtcgttata atcttattca aagcgaaaag cgcggccaat ggagcatgaa
    36001 gcagttcgat caatcctacg ccgagcagct gtttgagcta cgcgaaatgc tcgaaacaca
    36061 ttccctgcaa cactttctca accttcccga tgatgaccct cgctggttgc aggcgaaaac
    36121 catgctcgaa cgtcacagga cattgcgcga cagcattggc agcaactttc gaatgttttc
    36181 tcaattagac cgtgattttc atgccatgct gttatccgct gctgacaaca tcttttttaa
    36241 tcaatcactt gaaattatat cggtgatctt tcattttcac tatcaatggg atgaaagcga
    36301 tctcaaacag cgcaatatca ttgctgttga tgaacatatg accattctca gcgccctgat
    36361 ttgccgtagc gatttagatg caataacggc gttgcgaaac catctggata ccgccaaaca
    36421 atcaatgatt cgttccatca ggcaacatca ccgataaatt aagtacagat taaaaacatt
    36481 gattcttggt ttatatcact atgttgtatt aatgcagtat taatccaggc gattgttctg
    36541 cccataagga aatacatagg gaataaacga tgctggaagt attactctca gccatcattt
    36601 aattccctat tactggcagc gataaaatta tcggctatcc atgattaacg tttccagcac
    36661 atctaattca ctgactattt ttgccagtgt ttcatcactg acttcttgcc ttgcgcgcat
    36721 ctggtaaata gccgcgcgtt tggcatttaa cgccgccaga ttaaatgttc tttccagctt
    36781 cgcacgctgc atatgcaggg aattatcatt aggcgacata tcctcttcac tatgtcgcat
    36841 ataaccgata actctggccc ccgtttccat aatcaactcg ttatcgaggt tttctgttct
    36901 ggcgcgcagc aaacgttgct cggtcttttg cacactcagt atggccattt tagccataaa
    36961 cagatcggca gcttcctctt ctttttgtcg gttatcgctg aaaatcccca catttttccc
    37021 cagtaataac ggcagaataa taatgccgca aaccagcgaa aagaggatca cacctgtggc
    37081 gagaaatacc agcaagtaac gcccgggaaa aggtgtgcca tccggcagca ataacgggat
    37141 agaaagcacc cccgcaagcg tgatagctcc gcgcaccccc gcaaaggtcg ccagcagcat
    37201 atgacgcgtt ttatatccag caaacagcat cggatgtttg ccgggtcgtt tacggctata
    37261 cagacgcatc agccaaagcc agcccaggcg cacaaccatt aacgccaggt agatccaaat
    37321 gacatcaagg agcagcgatc cggtgtccac cagtggatcg ttttctgcac ggatcagtga
    37381 ttccgaaata atgcccggca gttgtaagcc taaaatcaga aacaccatgc cgttaaagac
    37441 atattcaagc atcgaccagc tacttttagc tttaattctc aggcgaatag gcgcgtggtg
    37501 gactacacca atgcgactgg ttgttaaccc cgcggcgaca gcggcaagaa tgccagataa
    37561 gccaaaatgt tccgcaacca gataaacaat aaacggcaac agaagcatca atacaatctg
    37621 aatggaaggt tcatcgtgag catatcggct gatcaacagc agccctttac catataacca
    37681 ggtaacagcg atccccacgg caataccgcc caacgcaacg acaaaaaacg caatggttgc
    37741 gccatgaacg ctgaattcca tcgttcccat cgtcactgca acagcaaatt tcaacgccac
    37801 caggccggaa gcatcattca tcaacgcttc gccttcgagc acttcctgga tgtttttgct
    37861 gatacgccct ttcccgacaa tccccagcaa cgccacggca tcggtaggcg acaacacggc
    37921 ggctaatgcg aaagcagcga tgagcggcac attcggtaag agtaaatgaa tcacatagcc
    37981 gacaccaaaa atagtgatta gtaccaacac aagtgccagg ctgataattt cccggctgtg
    38041 cttgatcaat tctttaggtt gtatttttga actttcagca aacaacaggg gtggtataaa
    38101 caataagaga aaaagttcag gattgaattc cacatgcaga ccaaatatag gctgggccag
    38161 tactgcgcca gcggcaatct ggattaaagg aagtggtatc tggacaggag acagacgtgt
    38221 aagcacgctg gttaatgata ctaccaacgt aagaattaaa attgttaaaa gtatttccat
    38281 ataataaaca ggcttcctca ttcataaata atggatatta ttaatttaac ttcataaaaa
    38341 gcgctctggt tagcacttaa cgttaatcat taatcactgc gttcactcac atgatcagaa
    38401 ggaacccagt aaaatgagtt ttcaagttga taacaaattc accacgtgaa tatttactgc
    38461 ctttccccgt tgtaacgaat catccgttac tctctgattt ccttgccgaa caagttattt
    38521 agtgaatgac ggttttcttt taatatcagc atgaattata cgttcagcaa atgtattaaa
    38581 tcagcactaa ataaatttaa tggataccac cattctgcgt caacacttag tttttatctg
    38641 tattgacaat ttaaaatggc cgcagccaaa ttaatgattt tatttttaat aaatcatcaa
    38701 cagaataaga gatcttatca gcggtaacta aactcatcag agggtgttcc ttgtaacgtc
    38761 atgcattaca taactttaag atatacaatc aaaaaatgca ttaaaaaccg attaaaagca
    38821 gcaaagtatt acccggtgca cgaatttatg atacatctgc agccattctg ctattcgctg
    38881 actacttttc agtaatgact gctttttcat aaggtttcac tcccgacaac aatgttggag
    38941 ttttatttcc ttagcatgga gtaagtaaag atgagcaaaa taatagcgat gctaattact
    39001 gtcagtaccc tttttttacc gtcaataatc caggcaaaac ctatttcaat aaaagttgcc
    39061 tatgaaaata accctggaga acccctggat gtggtcatgc gttactgggc agacctatta
    39121 aataaaaaaa gtaatggcga aataacactt gcgctctatc ccagctccca attgggatca
    39181 aaacaggatg tcaccgagca agccatgatg ggcatgaatg tcataacctt gagtgatgtg
    39241 gcttttcttg ccgattatga acccgatttg gggattttgt ttggaccgta tctgactgac
    39301 gatccgcaaa aactgttcaa aatatatgaa agcgactggt ttaaacaaaa aaatgaaagt
    39361 ctgaagaaaa agggaattca tgtggtaatg aataattatc tctatggcac tcgacagatt
    39421 atttcgaaaa aacccattcg caccgttgac gatctcgcag gtttaaaaat tcgcgttcct
    39481 aataacgtca tgcaaataaa agccattcag gcgatggggg caacaccaac tcccatgcca
    39541 ttaggcgaag tttatcccgc attaacccag ggcgttattg acggcgtaga aaacccaatt
    39601 tcagtgctgc aagggcaaaa actgtatgag caggcaagat atctgacaat ggttaactac
    39661 ctgacaaata cctcagtgtg gataggtggt gaagccttct ttagcaccct ttcaccagaa
    39721 caactggaga tgatccacca gaccggctat gaagcgggtg tctatagcca gaaactcact
    39781 cttgagcgtg atgccgaaat gttgaaaacc atgcaggcag ccggcgtcga agtgatctat
    39841 cccgatactg gaccattcca gaagaaagca cgcgaagttt actctcaatt cccggaatgg
    39901 acaccaggtc tgtacgaaac cattcaacag caactgcaat aagcattaat gctcagcgtc
    39961 ctgcctcatc tctcttaggc aggacacgca ctaacaggag gtttcatgcg tctttttggc
    40021 aaagtggcag aatttagtgc tgcctttgcg ctattcgttc tggttgtggt gacaattgga
    40081 gcggtattta tgcgttattt catcggccaa ccgcttcaat ggacagaaga aatgtcggga
    40141 atgctgatga tttgggtggt gatgttaggt ggagtggtgg cggaacgtga cagggcgcac
    40201 ctgaccattc ccttcctgat ggagatgctt ccaggcaaac tcaggcgtgt tattgcagta
    40261 ctggtcgccc ttctctccat cgcactgctg ctttatatgg cctggctggg ctaccgacta
    40321 gccgaaatgg cgcagtttaa agtgacacaa attctgaaag tttcctggtt ctggatcgat
    40381 ctggcggtcc ctgtcggcgc actggcaacg gctatttata ccctctactg gttaataaac
    40441 gatacaaaac aatccgatag cggagacaaa atgcaatgag ttggttattg attattccgc
    40501 taatgatcgt ctgtttgttt ctcaacattc gggtctatct ggcgatgttt attggcatca
    40561 tcgcttactt tttattcttc tccagcgtac cgattgaaat tgccgtgcaa agactaatcg
    40621 cgccaacgca aagcccatca ttgctggcaa ttccgttttt tatcttgctc ggcacactaa
    40681 tgagctacac cggcattgcc gaacgcatcc tgcacattgc caacgttctg gtggggaaaa
    40741 tgcgcggcgg actgggcgtg gcaaacattc tcgtcagcac catgatgggc ggcgtcagcg
    40801 cctctaacct tgctgacgcg gcgatgctgt cgcgcatgat ggtgccggag atggaacgta
    40861 aaggctacaa tcgcgcgttt gccgccgcca ttaccgctgg tggctcgtta gtcaccccga
    40921 tcataccgcc tggaatagcc ctgattattt acggtctggt tgccgatgtc tctatcggaa
    40981 aaatgtttat cgccggactg gtacctggtt tgctatgcgc agtcgtactg atgtttaccg
    41041 tctaccttgt cgcccgcaaa actaacgcta aaccctcgcg tgaaagctgg cctacgggca
    41101 aagagatggt gttctcgctg ggacaggcct ggcctgcgct gttcctcatt tttgctgtgg
    41161 tgggtggtat tcgcgctaac attttcaccc caacagaagc aggtgcagta gcggtgctaa
    41221 tcgtgctggc gattggcttt tttatctatc gtgaaatgcg gatttctcat gtcgtcaaag
    41281 cattgggcga aacggcacgc gcaaccgcat cggtgatgct ggtaatcatg gccagcgccg
    41341 cattgggatg gattttctca atggaacatg ctggtgtcgc agtggcgaat tttgtgactt
    41401 cgctgaccga aaacaaatac atgttcctgt tgattatcaa catattgctg ctgacgctgg
    41461 ggatgtttct ggaaggaaac gccattttgc tgatcctggt accactatta aaaccggtgg
    41521 tcgaacactt tggcatcgat ccggtgcatt ttggcatcat catgatcttc aacctgtcga
    41581 ttggcgcatt cacgccaccg gtaggtaccg tgatgctgct ggtatgtaat atcacgcaag
    41641 tttcggtggg aaatttcttt aaacaatccc tgcccctact ggcggctcta ctgttaatgc
    41701 taatgctggt gacatatatc ccggcgattt cactgttcct ggtttaataa taaaaacgcc
    41761 ggatgaaata ctcatccggc atcattacgt taaaacgttt cccagttctc ctcgctatct
    41821 gccacggcca ttttgcgcgg cgtagctggc gtcacggttt ttaccacagc cgatgcttca
    41881 cgctgctgtt gctgctgttg ctgaatcagg aacactgcca ctgcttcggt caggcgactg
    41941 gcctgttctt ccagcgcggc ggcggcggcg gcagattctt ccaccagcgc ggcgttctgt
    42001 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    42061 tcatcagaag cagaagcaat ttcgcccata atgtccgtca cgcgggtcac ggcactgaca
    42121 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    42181 accgagtctt caatcaggct tttaatttca cgagcagcct gagcgctacg ctgggccaga
    42241 ttacgcactt ctcccgcgac caccgcaaaa ccgcgtcctt gctcacccgc acgcgcagcc
    42301 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg caatgccgtc aattacgctg
    42361 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatggtctg caccacgtta
    42421 tccaccactt tgccgccgcg ttgcgccgtt tcggaagcac ttaacgccag atggctggcc
    42481 tggcgcgcat tttcggcatt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    42541 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcgtt attgccggtg
    42601 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    42661 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    42721 ccatccacct caatcggttt cactagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    42781 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaggatgacc
    42841 gccagtacga cgatcatcac gcctaccaga atccacatcg cctggctgta ggaggcattg
    42901 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    42961 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    43021 gcacctaaca gttgtatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    43081 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    43141 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    43201 gaaccgctac caatattgtt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    43261 ttacgcgtct gcaacaaagc gacccagctg ccattcagcg tggattgctg ctggcgaatg
    43321 gtttgtaaaa cagtgaaatt ttctttgtcg ttctttaagg cattaaagaa cagaccgcct
    43381 gaagtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaggctggt cacaattttg
    43441 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    43501 gaaaaacttt atgaaaatcc cgcggagatg acgccgagta attttattca ccccctgaat
    43561 ttaaaaagcc attatttaca taaattattc gcaatataaa tatgtgatat aaatcacata
    43621 tttatggtca cttaaacgac gcctttgccg ctcaccgcca ttcctgaccg cttacgtccc
    43681 taaaataacc actcagttat ttaccttact ttacgcgcgc gtaactctgg caacatcact
    43741 gcaggatagc ggtcaattta cctcctcaaa cgcaacgcaa acctagaacg gcttcggcca
    43801 actattaatc aatacatgcc aggttttact atggatacta aaaagatatt caagcacata
    43861 ccctgggtga ttctcggaat catcggtgca ttctgcctcg cggtagttgc attacgtcgg
    43921 ggggagcacg tcagcgccct gtggatcgtg gtcgcctctg tgtcggtgta tctggtggcg
    43981 tatcgctact acagtctgta catcgcccag aaggtgatga aactcgaccc cacgcgcgcg
    44041 acgcctgcgg ttattaacaa cgacggtctg aactacgttc cgaccaaccg ttacgtgttg
    44101 tttggtcacc acttcgccgc tatcgccggt gctggtccgc tggtgggtcc ggttctcgcc
    44161 gcgcagatgg gctacctgcc tggcacgctg tggctgctgg cgggggtcgt gctggccggt
    44221 gcggttcagg actttatggt gctgtttatc tcctctcgcc gtaacggcgc atctcttggt
    44281 gagatgatca aagaagagat gggaccagta ccggggacta tcgcgctgtt tggctgtttc
    44341 ttaatcatga tcatcatcct cgccgttctg gcactgattg tggttaaagc cctggccgaa
    44401 agtccgtggg gggtcttcac cgtttgctca accgtaccga ttgcgctgtt tatgggtatc
    44461 tacatgcgct ttattcgtcc ggggcgtgtg ggtgaagtct ctgtcattgg tatcgtgctg
    44521 ctggttgcct ctatctactt cggtggcgtg attgctcacg atccgtactg gggtccggca
    44581 ctgaccttta aagataccac cattactttc gcgctgattg gctatgcgtt tgtttccgca
    44641 ctgctgccag tgtggctgat cctcgcaccg cgcgactatc tggcaacctt cctgaaaatc
    44701 ggcgttatcg tcggcctggc gctgggtatc gtggtgctga acccggaact gaaaatgcct
    44761 gccatgaccc agtacattga cggtactggc ccgctgtgga aaggcgctct gttcccgttc
    44821 ctgttcatca ccatcgcctg tggtgcggta tctggcttcc acgcgctgat ctcttccggt
    44881 acgacgccga aactgctggc taatgaaacc gacgcgcgtt tcatcggcta cggcgcaatg
    44941 ctgatggagt ccttcgtggc gattatggcg ctggttgctg cgtccatcat cgaaccgggt
    45001 ctttacttcg cgatgaacac cccgcctgct ggcctcggca tcaccatgcc taacctgcat
    45061 gaaatgggtg gcgagaacgc gccgatcatc atggcacagc tgaaagacgt taccgcacac
    45121 gcggcagcga ccgtcagctc ctggggcttc gtgatttcgc cagagcagat cctgcaaacc
    45181 gcgaaagaca ttggtgagcc ttctgtcctg aaccgtgcag gtggtgcgcc aacgctggcg
    45241 gtaggtatcg ctcacgtgtt ccacaaagtg ctgccgatgg ctgacatggg cttctggtat
    45301 cacttcggta ttctgttcga agccctgttc atcctgaccg cgctggatgc gggtacccgt
    45361 tctggccgct ttatgctgca agacctgctg ggtaacttca tcccgttcct gaaaaaaacc
    45421 gactctctgg ttgccggtat catcggtact gcgggctgtg tgggtctgtg gggctacctg
    45481 ctgtatcagg gcgtggtcga tccgctgggc ggcgttaaga gcctgtggcc gctgttcggt
    45541 atctccaacc agatgctggc agccgtggcg ctggtactgg gcaccgttgt actgattaag
    45601 atgaagcgca cccaatacat ctgggttacc gttatcccgg cagtatggct gcttatctgc
    45661 accacctggg cgctgggcct gaaactgttc agcaccaacc cgcagatgga aggcttcttc
    45721 tacatggcga gccagtacaa agagaagatt gctaacggta ctgacctgac ggcgcagcag
    45781 attgccaaca tgaaccacat cgttgtgaac aactacacca acgcaggcct gagtattctg
    45841 ttcctgattg tggtgtacag catcatcttc tacggtttca aaacctggct gaaggtgcgt
    45901 aacagcgaca aacgtactga caaagaaaca ccgtacgttc caatcccgga aggcggcgtg
    45961 aagatctctt cgcaccacta accggttgcc ttatccggct tacaaaatgt ctatcgtagg
    46021 ccggataagg cgtttacgcc gcatccggca aatccccagc ccggaccttc cgggctttac
    46081 gcatatctgg aaggcaaaat gtttggtaac ttaggacagg caaaaaaata tctcggccag
    46141 gcggcgaaga tgttgattgg cattccagac tacgacaact acgtcgagca tatgaagacc
    46201 aaccatcccg acaagccgta catgagctat gaagaattct tccgcgaacg ccagaacgcg
    46261 cgctacggcg gcgatggtaa aggcggtatg cgctgttgtt aatggagaga gcatgaaccc
    46321 gattgcagtt accctactca ccggtttttt aggcgcagga aaaaccaccc tgctgcgcca
    46381 tattcttaac gaacaacacg gctacaagat tgccgtgatt gaaaacgaat tcggcgaagt
    46441 ctccgttgat gatcaactga ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg
    46501 ctgcatctgc tgttcgcgct ccaacgagct ggaagacgcc ctactcgacc tgctggataa
    46561 tctcgacaag ggcaatattc agttcgaccg tctggtcatt gaatgcaccg gcatggccga
    46621 tcccggcccg attattcaga cctttttctc ccatgagatt ttatgccagc gttacctgct
    46681 ggatggcgtg attgcgctgg tggatgcggt acatgccgat gagcagatga accagttcac
    46741 catcgcccag tcccaggttg gctacgccga ccgtattctg ctgactaaaa ccgacgtcgc
    46801 aggcgaagca gaaaaactgc gtgaacgcct ggcgcgcatc aacgcccgtg caccggtcta
    46861 caccgtcacc cacggcgaca tcgacctggg cctgttgttc aacaccaacg gttttatgct
    46921 ggaagaaaac gtcgtcagca ccaagccacg ttttcacttt atcgcggaca aacaaaacga
    46981 tatttcgtcg attgtggtgg aactggatta cccggtagat atcagcgaag tttcccgcgt
    47041 gatggaaaac ctgctgctgg aatcggcaga taaactgctg cgttacaaag ggatgctgtg
    47101 gattgacggc gaacctaacc gcctgctgtt ccagggcgtc cagcgcctct acagcgccga
    47161 ctgggacagg ccgtggggcg atgaaaaacc gcatagcacg atggtgttta tcgggattca
    47221 gctgccggag gatgagattc gggcggcgtt tgcggggttg aggaagtaag gggttatggg
    47281 ccggataagg cgcagccgca tccggcctga tatttcactc aaaataatcc atatccagtt
    47341 tcggcacctt caacaaacgt gaagaaaccc ctacttcgtt ttcgatcatt aagtgcacca
    47401 ggcgttcccc atcaaccaac accataccct cgacggattg ggcaaagtca cgcgcctgag
    47461 aagtaaatcc agaagtggta ataaacaccc cacgtttcgc tttttgccca gccagtgcgc
    47521 cgtaaaatgc ctgtaattct ggcctgccta cagtattctg ccaacgtttt gcctgaacat
    47581 aaactttctc caggccaagt ttatcaagcg atatcacacc atcgatgcca ccatctccag
    47641 taccgccaac acgctgcaaa tcatcacggt ggccgccata ccccaggcga tgcaaaacat
    47701 ccagaacaat gacttcaaag cgcgaaggag aaacctgcaa taagttttcc agaacctcat
    47761 cagccaccgc atcacgaagc tcttttagcg cctgatctaa ccgatcgtcc gggctgctct
    47821 ttgcaagttc ttcatgatcg ggagagtcgg ctttcggatc taaatcgacg gcatccggcc
    47881 gtgacttaag tttgacattc acaaaattga aggccagatg gttcatctcc tgctccgtca
    47941 ttggctgggg atgagacgca acccagtcaa aacccgcagg agtcaggcac catttgccac
    48001 gcgacaaact ttgcgacaac ccggcacgtt ttaaacggtc atgtgcccag cctgcacgat
    48061 ttttataaac aagttgtccg ctggtaatga ctttcgatcg ctggctgtca tccagtccta
    48121 atgcatccgc ggcagcctca tgaacatcac gcgcggctgc accttccggt tttgttgcca
    48181 gataacgcag aacaggttca ataaatttgt cataggtagg aaccgtcata gtacatcctt
    48241 gcagaatcag gtagatgttt ttcggctact ataacactac caaaatagac gaacacgtta
    48301 gaaatgagtc agttgctgtg actccggtca ttgcccggaa aggtacagaa agctaagata
    48361 gggcgttatg ggccttaaat atttggacag gcccacacag caatggatta ataacaatga
    48421 tgaataaatc caacttcgaa ttcctgaagg gcgtcaacga cttcacttat gccatcgcct
    48481 gtgcggcgga aaataactac ccggatgatc ccaacacgac gctgattaaa atgcgtatgt
    48541 ttggcgaagc tacagcgaaa catcttggtc tgttactcaa catcccccct tgtgagaatc
    48601 aacacgatct cctgcgcgaa ctcggcaaaa tcgccttcgt tgatgacaac attctctctg
    48661 tattccacaa attacgccgc attggtaacc aggcggtgca cgaataccat aacgatctcg
    48721 acgatgccca gatgtgcctg cgactcgggt tccgcctggc tgtctggtac taccgtctgg
    48781 tcaccaaaga ttatgacttc ccggtgccgg tatttgtgtt gccggaacgt ggcgaaaacc
    48841 tctatcacca ggaagtgctg acgctaaaac aacagcttga acagcaggcg cgagaaaaag
    48901 cgcagactca ggcagaagtc gaagcgcaac agcagaagct ggttgccctg aacggctata
    48961 tcgccattct ggaaggcaaa cagcaggaaa cagaagcgca aacccaggct cgccttgcgg
    49021 cgctggaagc acagctcgcc gagaagaacg cggagctggc caaacagacc gaacaggaac
    49081 gtaaggctta ccacaaagaa attaccgatc aggccatcaa gcgcacactc aaccttagcg
    49141 aagaagagag tcgcttcctg attgacgcgc aactgcgtaa agcaggctgg caggccgata
    49201 gcaaaaccct gcgcttctcc aaaggcgcac gcccggaacc cggcgtcaat aaagccattg
    49261 ccgaatggcc gaccgggaaa gatgaaacgg gtaatcaggg ctttgcggat tatgtgctgt
    49321 ttgtcggcct caagcccatc gcggtggtag aggcgaaacg taaaaatatc gacgttcccg
    49381 gcaagctcaa tgagtcgtat cgctacagca aatgcttcga taatggcttc ctgcgggaaa
    49441 ccttgcttga gcattactca ccggatgaag tgcatgaagc tgtgccggag tatgaaacta
    49501 gctggcagga caccagcggc caacaacggt ttaaaatccc cttctgctac tccaccaacg
    49561 ggcgcgaata tcgcgcagca atgaagacta aaagtggcat ctggtatcgc gacgtgcgtg
    49621 atacccgcaa tatgtcgaaa gccttacccg agtggcatcg cccggaagag ctgctggaaa
    49681 tgctcggcag cgaaccgcaa aaacagaatc agtggtttgc cgataacccg ggcatgagcg
    49741 agctgggcct gcgttattac caggaagatg ccgtccgcgc ggtggaaaag gcaatcgtca
    49801 agggtcaaca agagatcctg ctggcgatgg cgaccggtac cggtaaaacc cgcaccgcaa
    49861 ttgccatgat gttccgcctg atccagtccc agcgttttaa acgcattctc ttccttgtcg
    49921 atcgccgttc tcttggcgag caggcactgg gggcgtttga agatacgcgt attaacggcg
    49981 acaccttcaa cagcattttc gacattaaag ggctgacgga taaattcccc gaagacagca
    50041 ccaaaattca cgttgccacc gtacagtcgc tggtgaaacg caccctacaa tcagatgaac
    50101 cgatgccggt ggcccgttac gactgtatcg tcgtcgatga agcgcatcgc ggctatattc
    50161 tcgataaaga gcagaccgaa ggcgagctgc agttccgcag ccagctggat tacgtttctg
    50221 cctaccgtcg cattctcgat cacttcgatg cggtaaaaat cgccctcacc gccaccccgg
    50281 cgctgcatac ggtgcagatt ttcggcgagc cggtttaccg ttatacctac cgtaccgcgg
    50341 ttatcgacgg ttttctgatc gaccaggatc cgccgattca gatcatcacc cgcaacgccc
    50401 aggagggggt ttatctctcc aagggcgaac aggtagagcg catcagcccg cagggagaag
    50461 tgatcaacga taccctggaa gacgatcagg attttgaggt cgccgacttt aaccgtggcc
    50521 tggtgatccc ggcgtttaac cgcgccgtct gtaacgagct caccaactac cttgacccga
    50581 ccgggtcgca aaaaacgctg gtcttctgcg ttaccaatgc ccatgccgat atggtggtgg
    50641 aagagttgcg taccgcgttc aagaaaaagt atccgcaact ggagcacgac gcgatcatca
    50701 agatcaccgg tgatgccgat aaagacgcgc gcaaagtgca gaccatgatc acccgcttca
    50761 ataaagagcg gctgcccaat atcgtggtaa ccgtcgacct gctgacaacc ggcgtcgata
    50821 ttccgtcgat ctgtaatatc gtgttcctgc gtaaagtacg cagccgtatt ctgtacgagc
    50881 agatgaaagg ccgcgccacg cgcttatgcc cggacgtgaa taaaaccagc tttaagattt
    50941 tcgactgtgt cgatatctac agcacgctgg agagcgtcga caccatgcgt ccggtggtgg
    51001 tgcgtccgaa ggtggaactg caaacgctgg tcaatgaaat taccgattca gaaacctata
    51061 aaatcaccga agcggatggc cgtagctttg ccgagcacag ccatgaacaa ttggtggcga
    51121 agctccagcg catcatcggc ctggccacgt ttaaccgtga ccgcagcgaa acgatagata
    51181 aacaggtgcg tcgtctggat gagctatgcc aggacgcggc gggcgtgaac tttaacggct
    51241 tcgcctcgcg cctgcgggaa aaagggccgc actggagcgc cgaagtcttt aacaaactgc
    51301 ctggctttat cgcccgtctg gaaaagctga aaacggacat caacaacctg aatgatgcgc
    51361 cgatcttcct cgatatcgac gatgaagtgg tgagtgtaaa atcgctgtac ggtgattacg
    51421 acacgccgca ggatttcctc gaagcctttg actcgctggt gcaacgttcc ccgaacgcgc
    51481 agccggcatt gcaggcggtg atcaatcgcc cgcgcgatct cacccgtaaa gggctggtcg
    51541 agctccagga gtggtttgac cgccagcatt ttgaggaatc ttccctgcgc aaagcatgga
    51601 aagagacgcg caatgaagat atcgccgccc ggctgattgg tcatattcgc cgcgctgcgg
    51661 tgggcgatgc gctgaaaccg tttgaggaac gtgtcgatca cgcgctgacg cgcattaagg
    51721 gtgaaaacga ctggagcagc gagcaattaa gctggctcga tcgtttagcg caggcgctga
    51781 aagagaaagt ggtgctcgac gacgatgtct tcaaaaccgg caacttccac cgccgcggcg
    51841 gtaaggcgat gctacaaaga acctttgacg ataatctcga caacctgctt gataaattca
    51901 gcgattatat ctgggacgag ctggcctgac acgtatacac ttcatccttc aggctgcctc
    51961 tgcgttggct gcgctcgttc accccggtca cgtacttctg tacgctcccg gggattcact
    52021 cacttgccgc cttgatgcaa cctgaatgat tttgtgtata ttaccctcgg caatttcttc
    52081 ttctgcggcc ccaaacaatg cgggccgttg tttaatttac ggaactcaca atgaacaata
    52141 acgatctggt cgcgaagctg tggaagctgt gcgacaacct gcgcgatggc ggcgtttcct
    52201 atcaaaacta cgtcaatgaa ctcgcctcgc tgctgttttt gaaaatgtgt aaagagaccg
    52261 ggcaggaagc ggaatacctg ccggaaggtt accgctggga tgacctgaaa tcccgcatcg
    52321 gccaggagca gttgcagttc taccgaaaaa tgctcgtgca tttaggcgaa gatgacaaaa
    52381 agctggtaca ggcagttttt cataatgtta gtaccaccat caccgagccg aaacaaataa
    52441 ccgcactggt cagcaatatg gattcactgg actggtacaa cggtgcgcac ggtaagtcgc
    52501 gcgatgactt cggcgatatg tacgaagggc tgctgcagaa gaacgccaat gaaaccaagt
    52561 ctggcgcggg ccagtacttc accccgcgtc cgctgattaa aaccattatt catctgctga
    52621 aaccgcagcc acgtgaagtg gtacaggatc cggcagccgg tacggcgggc tttttgattg
    52681 aagccgaccg ctacgttaag tcgcagacta acgatctgga cgaccttgat ggcgacacgc
    52741 aggatttcca gatccaccgc gcgtttatcg gcctcgaact ggtgcccggc acccgtcgtc
    52801 tggcgctgat gaactgcctg ctgcacgata ttgaaggcaa cctcgaccac ggcggcgcaa
    52861 tccgtctggg caacaccctg ggcagcgacg gcgaaaacct gccgaaggcg catattgtcg
    52921 ccactaaccc gccgtttggc agcgccgcag gcaccaacat tacccgcacc tttgttcacc
    52981 cgaccagcaa caaacagttg tgctttatgc agcatattat cgaaacgctg caccccggcg
    53041 gtcgtgcggc ggtggtggtg ccggataacg tgctgtttga aggcggcaaa ggtacggaca
    53101 tccgtcgtga cctgatggat aagtgtcatc tgcacactat tctgcgtctg ccgaccggta
    53161 ttttttacgc gcagggcgtg aagaccaacg tgctgttctt taccaaaggg acggtggcga
    53221 acccgaatca ggataagaac tgcaccgatg acgtgtgggt gtacgacctg cggaccaata
    53281 tgccgagctt tggtaaacgc acgccgttta ccgacgagca tttgcaaccg tttgagcgcg
    53341 tgtttggcga agacccgcac ggcttaagcc cgcgcactga aggtgaatgg agttttaacg
    53401 ccgaagagac ggaaattgcc gacagcgaag agaacaaaaa caccgaccag catcttgcta
    53461 ccagccgctg gcgcaagttc agccgcgagt ggatccgcac cgcgaaatcc gattcgctgg
    53521 atatctcctg gctgaaagat aaagacagta ttgatgccga caacctgccg gagccggatg
    53581 tattagcggc agaagcgatg ggtgaactgg tacaggcgct gtctgaactg gatgcgctga
    53641 tgcgtgaact gggggcgagc gatgaggcgg atgcgcagcg ccagttgctg gaagaagcat
    53701 ttggtggggt gaaggaatga gtgctgggaa attgccggag gggtgggaac agattgaaat
    53761 aggcgacatt gctgatgtca tctctggtgg gacaccaaaa tcgggagtcg ctgaaaattt
    53821 tgcaccttcg ggtgagggag tcgcctggct tacgcctgcc gatttgagcg gttataaaga
    53881 gaaatatatc tcacatggtg ccagagattt aactacacta ggctattcct cttgttcagc
    53941 aaaactaatg cccaaaggca ctatactttt tagtagccgg gcaccgattg gatatgttgc
    54001 tattgctgca aatgagattg cgactaacca gggtttcaaa agttttgctt ttccttcaga
    54061 catttttcct gattatgcat attactttct ccgcaacatc agacatattg cagaggaaat
    54121 gggcacagga acaactttta aagaaatttc tggtagttct gcgaaaacat taccatttgt
    54181 tcttgtaccg tttgccgaac aaaaaatcat cgctgaaaaa ctcgatacgc tgctggcaca
    54241 ggtagacagc accaaagcac gtcttgagca aatcccacaa atcctgaaac gttttcgtca
    54301 ggcggttctg ggtgcagcgg tgagaggaaa actaactgaa gactggagag ataattcaag
    54361 cctgtctggg tggagagaag gcaaacttgg ggagtttata aaaaaaccaa gttacggaac
    54421 gtcatctaag tctaataaag aaggactcat tcctgtatta agaatgggca atcttcaggg
    54481 aggaaagttg gactggacgg atctagtcta tacgtcagat accatagaaa ttgaaaagta
    54541 taaacttgag tacaatgatg tattattcaa tcgtacaaat agtcctgaac ttgtcgggaa
    54601 aacagccata tataaatcag agcaaccagc tatttatgca ggttatttga ttagggttca
    54661 atgtttacct gatttaaacc ctgactatct taactaccat ttaaatagca ttttggggag
    54721 acaatattgc tattcagtta aatctgatgg ggtcagtcaa tcaaatatta atgcacagaa
    54781 attaattgca tacccaataa ctgttccccc ccttcccgaa caagctgaaa tcgttcgccg
    54841 cgtcgagcaa ctcttcgcct acgccgacac aatagaaaaa caggtcaaca acgccttagc
    54901 ccgcgtcaac aatctgacgc aatccatcct ggcaaaagcg ttccgtggtg aactcaccgc
    54961 ccagtggcgg gccgaaaacc cggaattaat cagcggtgaa aacagcgccg ccgcgttgct
    55021 ggagaaaatc aaagctgaac gcgcagccag cgggggtaaa aaagcctcgc gtaaaaagtc
    55081 ctgatcatta ttttctggcg cacctttctg gtgcgctatt tattattttt acgccaatca
    55141 taactcacac aaatatattt aaatcgttcc agaaacttcc cattttattc tatttttagc
    55201 tggactttcc ctgtatttac tgatgatata tccaggtatt tagcgcgatg cggatgtgcg
    55261 ccaacacacc cgcaccgctc atcacaatca ctatttatgg agaatagtaa tcatgactga
    55321 cacgcattct attgcacaac cgttcgaagc agaagtctcc ccggcaaata accgtcaatt
    55381 aaccgtgagt tatgcgagtc gctacccgga ttacagccgt attcccgcca tcaccctgaa
    55441 aggtcagtgg ctggaagccg ccggttttgc cactggcacg gcgatagatg tcaaagtgat
    55501 ggaaggctgt attgtcctca ccgcccaacc ccccgccgcc gaagagagcg aactgatgca
    55561 gtcgcttcgc caggtgtgca agctgtcggc gcgtaaacaa aggcaggtgc aggagtttat
    55621 tggggtgatt gcgggtaaac agaaagtcgc ctgatacatt gacccggtag cgccagtgct
    55681 accgggtatt attcgccacg ttataccaac gcgctctcca tctgtttttc aaatgcctga
    55741 cgaattaatc ccataatgta aggaagttgt gcgaggtcac tgaaaccaat ttccacatca
    55801 ccattgcccc atcgcccaac atttgtcaca tctttggcaa tacctttcgg atcgaccagt
    55861 tcatgaaact gcatattaag cgacaaccgc agtcggcttt tttgcggtac aacatcaaca
    55921 aaattcgttt cagctttaaa cgcaatatag agctttaaaa cttcctgcgt gatccctgcg
    55981 tccaggcgca taacttcatc gcgaagatga tcaaataaca cccggctatg cgaaccatca
    56041 gcaagaaaag gataatcact caggctgtaa ctggttttgt tctccggctg cgcccgataa
    56101 acagctaaaa cctcttcagg aagagcaggc gcttgccaga cctgaaccgc gagatcggcc
    56161 agacgatctg cacgggcgcg aatggctgct tcatcccatt tttcgcactg tccaagaccg
    56221 atattcaaat agagcgggct atgtttaaag ccgtcttcaa tatcgcgttt ttccgcgaaa
    56281 ggtctgtcac tgtagcggga gttatagcgc gttaatgtga gattccccaa cgtatgcaac
    56341 agttctttat gaatgcgttg ccagtcactt cccagctctt cacgccattt agccgacaaa
    56401 ttttcgttct gcggcataat atgctcaatc gtaaactctt ccacgcgctc tctgcgttta
    56461 tcgttttcca gtcgtcgtaa ccagtagctg cgactacgga aattatagag atcacgaact
    56521 tttaattccc gtttaaattc atcatcgttg gggaaacgac gatatgaagg cagattcaga
    56581 aaatgtacct gaatactttc cagatatttt tctttattaa tgaccttata aaaagtagca
    56641 aacgtcttgt taagagaatt tgtcggaatc gcacatactg cacggcggaa aacataagat
    56701 tcaattaaac gaattatgct caggaaatct tcgtgagaca aaacgccatt tttatagtca
    56761 tgataaagcg ccagtaagaa aggatacgcc acatcgacct ttaactcgcg caaatcctga
    56821 aaagccgtag caagcgattt gtcactttct tttcccaatg ccattgcaca gtaatactcc
    56881 gcgtagatgt gaatatcttc aaccagccga tctacgcctt tttcagcaac actctggctg
    56941 cgggcatgga gtttaaatgc ctcatagact tcatctgtcc gagggatctc ccccgttttt
    57001 accgtcagat aatgacgcat aaaactgtca aaatattcgc tgtaaccttg ctgaccaaac
    57061 gcgacttcca ttggacgcca gtgatcttca tacaaccggg tttgatgctc tggttcgagg
    57121 cccatcagaa taaaattgcg caccagatcg gcctgactta atgccttacc ggtggagttc
    57181 atactttcaa aaatcagttg cggattatcc tgaccacgat taagcgccac atcgacaatt
    57241 aataactttg ctaacccacg acataagggg atcaagtcat caccgagttt ggtaatttgt
    57301 tcatcaaaga aagtaaagtt ttccattatt cgatgggaat agttttctgg cattggtctt
    57361 tgttttatta acgccagcaa actgtcttta tcagtctcgg tcagcagtaa tttaaatcct
    57421 ttctcgccgg actcataagg attgagtaaa taataattac gcaatttcat tgctgaaaag
    57481 ccatcaaaaa cttcgtcttc gccaagatga cgcgataaag cctcaatcag caacattgcg
    57541 gtcgtcagcc tttgttgacc atcaatgacc agtaacggag aaataccaga aacctgatac
    57601 aacccctgct caatataaac aaccgaaccg ataaaatgcg ctgatatatc gtcacgcttt
    57661 ccggcacgaa tgatgtcgtc ccaaagttgc cgacattgtt gttctgtcca tgaataagtc
    57721 cgttgataaa tggtaataac gaactgctgc gaacgtttta aaaaatcgag taggcgggct
    57781 tctgtagctt tcattctgac gtctttccgt gaagcgatga tttcgcaata ataacccctt
    57841 atctgacgag gtgacatata aatcccatcg tttcgtgaag gactctctcc attacaccac
    57901 atcctccctg cataacccaa tatcctttaa ccgttcatct ctcacctgct gtaacacgca
    57961 ccgggtctgc atttgctgcc accagcagcg ttaatgtacc gttcacataa atggcggcgg
    58021 caacgttggt gcaggaaggt cactatcatc gccacgtaaa gtgtgcttcg ttcatgccgg
    58081 atgcggcgtg aacgccttat ccggcctaca aaccgcgcaa attcaataga ttgcagagat
    58141 aatgtaggtc tgataagcgt agcgcatcag gcagctttgc gtttgtcatc cgtctctgat
    58201 tagctcttga gccatgaata aggctcacgc tcaggcaagg gctgctctaa tgcctgtatt
    58261 tgctctgccg aaagtccggt aatcttttgt atccattcag gatctacccc attctgcaaa
    58321 aacaatcgaa gaatgcgctg ttcccctttt atatatccat cattatgaat tcgctctgca
    58381 atcgtcatta ttcgctccct ttgttgtggc agtcgacggg taatttcact gataaactca
    58441 ttaaaacgcg cttcatcacc agtcagtaaa atgtaattta acagcgcagt tatctggctg
    58501 tcattagcac actctgtaac cagtaatact accagttggt caataagccc catcagatcg
    58561 cgctggcgaa tatgcttttg gatcaactcc aacagggcga ctctgcgatg ctgcacaatc
    58621 tcgtcgtctg gcacgacagt gatatccacc agcgggaacg cggcggtata aagcttccgt
    58681 gcggtagttg ggtcggcaaa ttcgtccagc cagcacaaag accagggata agggctacgg
    58741 ctaccgtgat aaaacagcat cgggatgacc agtggtagct gccggcgttt atcatgttcg
    58801 atatggcgct gcatcaccgc catggaatat cgcatcagac gaaaggccat atgaatatcc
    58861 tcacggctct gatgttcaat cactacataa atatagccat cgccttcacg ggtctttacc
    58921 gaccacagaa tatcggaatg tagcgcccgc aatttttcat cgacaaagct ggcggattcc
    58981 agttttaagc tatcgagatc gcacagttca cgtaaatctt tgggtaagtg gatctccata
    59041 aaatcccgcg cggtgtcaga gtgtgtgaga aaggatttaa ataatgcgtc atgcggcgtg
    59101 ctggtcgtaa agtttgtcat ggcgatccgt cacctgtaga taagatggcc caactttagc
    59161 gactaacaaa gccattcaca cctggctatt tcctattccc cgaggcggtt tccaggctat
    59221 ttttattctt ctgcaaacga tacattttta tggaagatat ctcgcttttt gcgttttctt
    59281 accgtcaggc tctattctta cgatatgaat aaactcattg aactcagacg cgccaaaatg
    59341 ttggcgctct ctttactgct tatcgccgct gctacctttg tcgttacgct gtttttgccg
    59401 cccaattttt gggtgagcgg tgtgaaggcg attgctgaag cggcgatggt cggcgcgctg
    59461 gcggactggt ttgcggtggt ggcgctgttt cgccgcgtgc cgattccgat catttctcgt
    59521 catacggcga ttatcccgcg taataaagac cggattggcg aaaatctcgg ccagttcgtg
    59581 caggaaaaat ttctcgatac ccaatcgctg gtggcattga ttcgacgtca cgaaccggcg
    59641 ttgttgattg gcaactggtt tagtcagccg gagaacgccc gccgcgttgg tcagcatctg
    59701 ttacagatca tgagcggttt tctcgaactg accgatgatg cgcgtattca gcgcctgctt
    59761 aagcgcgcgg tccaccgggc gattgataaa gtcgatcttt ctggcaccag tgcgttgatg
    59821 ctggagagta tgaccaaaaa cgatcgtcat caggtgctgc tggatacgct gatcgcacag
    59881 ttgatcgccc ttctccagcg cgataaatca cgcaagttta ttgcccagca aattgttcgc
    59941 tggctggaga gcgagcatcc actgaaagcc aaaattttgc ccactgaatg gctgggcgaa
    60001 catagcgcgg agttggtttc tgacgcggtg aattctttgc ttgatgatat cagccgcgat
    60061 cgtgcgcatc agatccgcca tgcgtttgat cacgccacct ttgccctgat cgacaagctg
    60121 aaaaacgatc cggaaatggc agcgcgagcc gatgccgtaa aaagctatct gaaagaagat
    60181 gaggccttta accgctatct cagtgaattg tggggggatt tacgggaatg gctgaaagcg
    60241 gatatcaaca gtgaagattc tcgtgtgaaa gaacgtatcg cgcgggcggg gcaatggttt
    60301 ggcgaaacgt taattgccga tgatgccttg cgggcgtcgt taaatggtca tctcgaacaa
    60361 gccgcacacc gcgttgcgcc tgagttttcc gcattcctga ctcgccatat cagcgacacg
    60421 gtaaaaagct gggacgcacg ggatatgtcg cggcaaatcg agttaaatat cggcaaagat
    60481 ctccagttta tccgtgtcaa cggtacgctg gttggcggtt gtattggact tattttgtat
    60541 ttgctgtcgc agctcccggc cttgttcccc ctcggcaatt tatagaaatc catgaaaaac
    60601 taagtggcta accgcgagag agatcaaata aaccacacta aaagaatggt aataatgtgg
    60661 cctcttttca ttatctcccg tggtacgggg aaggaaaatc atgtcacttg tcaccgatct
    60721 acccgccatt ttcgatcagt tctctgaagc tcgccagaaa ggctttctca ccgtcatgga
    60781 tctcaaggag cgcggcattc cgctggttgg cacctactgc acctttatgc cgcaagagat
    60841 cccgatggca gccggtgcgg ttgtggtttc gctatgttcc acctctgatg aaaccattga
    60901 agaagccgag aaagatctgc cgcgcaacct ctgcccgctg attaaaagca gctacggctt
    60961 cggcaaaacc gataaatgcc cctacttcta cttttctgat ctggtggtcg gtgaaaccac
    61021 ctgcgacggc aaaaagaaaa tgtatgaata catggcggag ttgattagac ttgagataaa
    61081 tgacagggaa taagtgagat atttatggga agaactgggt caaaatggga agagactagg
    61141 aggaatatgc aataacctta cagtatgtga aaatcagacc ggcccgaaaa aaaaacagaa
    61201 ctcgcgccag cctgattgca gatattactc ttttttctcc gtatct
//
LOCUS       DAPOZO010000019        58035 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPOZO010000019 DAPOZO010000000
VERSION     DAPOZO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.019"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            160..1059
                     /gene="lysO"
                     /locus_tag="ROI21_001261"
     CDS             160..1059
                     /gene="lysO"
                     /locus_tag="ROI21_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDX5110208.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNYHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1203..2855
                     /gene="hcp"
                     /locus_tag="ROI21_001262"
                     /gene_synonym="priS"
     CDS             1203..2855
                     /gene="hcp"
                     /locus_tag="ROI21_001262"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDX5110209.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETSKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            2867..3835
                     /gene="hcr"
                     /locus_tag="ROI21_001263"
     CDS             2867..3835
                     /gene="hcr"
                     /locus_tag="ROI21_001263"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDX5110210.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRSPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3968..5686
                     /gene="poxB"
                     /locus_tag="ROI21_001264"
     CDS             3968..5686
                     /gene="poxB"
                     /locus_tag="ROI21_001264"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDX5110211.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATSHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5723..6724
                     /gene="ltaE"
                     /locus_tag="ROI21_001265"
     CDS             5723..6724
                     /gene="ltaE"
                     /locus_tag="ROI21_001265"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDX5110212.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAIVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6735..8165
                     /locus_tag="ROI21_001266"
     CDS             6735..8165
                     /locus_tag="ROI21_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDX5110213.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPFPTRWISVWFLNVITSVPPTTAKALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            8264..9277
                     /locus_tag="ROI21_001267"
     CDS             8264..9277
                     /locus_tag="ROI21_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDX5110214.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9274..10104)
                     /gene="amiD"
                     /locus_tag="ROI21_001268"
     CDS             complement(9274..10104)
                     /gene="amiD"
                     /locus_tag="ROI21_001268"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDX5110215.1"
                     /translation="MRRVFWLIAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETHAIAEALLEKYGQD
                     "
     gene            complement(10101..10424)
                     /locus_tag="ROI21_001269"
     CDS             complement(10101..10424)
                     /locus_tag="ROI21_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDX5110216.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10635..12404)
                     /locus_tag="ROI21_001270"
     CDS             complement(10635..12404)
                     /locus_tag="ROI21_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDX5110217.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDELLAENIPSANYQFNIFFSNAFEIDVKKYLGKSEDN
                     EVEIKVGNERITIGIRNISNGKLERQKFLFPLDYENKLQHQLDKYFTIESHPLLYRHT
                     MGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIAKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYNDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDATNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIVNNVFKNVSFRTVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMIAGNNVKVAADAFL
                     EILYSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     IPQAQDGEDFRNHLQYKLTLPTH"
     gene            13052..13567
                     /locus_tag="ROI21_001271"
     CDS             13052..13567
                     /locus_tag="ROI21_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX5110218.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRNNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            13785..14513
                     /gene="artP"
                     /locus_tag="ROI21_001272"
     CDS             13785..14513
                     /gene="artP"
                     /locus_tag="ROI21_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDX5110219.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALTRAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            14531..15262
                     /gene="artJ"
                     /locus_tag="ROI21_001273"
     CDS             14531..15262
                     /gene="artJ"
                     /locus_tag="ROI21_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX5110220.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            15269..15985
                     /gene="artQ"
                     /locus_tag="ROI21_001274"
     CDS             15269..15985
                     /gene="artQ"
                     /locus_tag="ROI21_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDX5110221.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            15985..16653
                     /gene="artM"
                     /locus_tag="ROI21_001275"
     CDS             15985..16653
                     /gene="artM"
                     /locus_tag="ROI21_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDX5110222.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLAVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            16879..17610
                     /gene="artJ"
                     /locus_tag="ROI21_001276"
     CDS             16879..17610
                     /gene="artJ"
                     /locus_tag="ROI21_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDX5110223.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPHYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(17639..18766)
                     /gene="rlmC"
                     /locus_tag="ROI21_001277"
     CDS             complement(17639..18766)
                     /gene="rlmC"
                     /locus_tag="ROI21_001277"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDX5110224.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAVLK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVNHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACANQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVQQ"
     gene            complement(18807..19295)
                     /locus_tag="ROI21_001278"
     CDS             complement(18807..19295)
                     /locus_tag="ROI21_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDX5110225.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(19355..20200)
                     /gene="potI"
                     /locus_tag="ROI21_001279"
     CDS             complement(19355..20200)
                     /gene="potI"
                     /locus_tag="ROI21_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDX5110226.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDSSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(20197..21141)
                     /gene="potH"
                     /locus_tag="ROI21_001280"
     CDS             complement(20197..21141)
                     /gene="potH"
                     /locus_tag="ROI21_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDX5110227.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLMIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(21151..22284)
                     /gene="potG"
                     /locus_tag="ROI21_001281"
     CDS             complement(21151..22284)
                     /gene="potG"
                     /locus_tag="ROI21_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDX5110228.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFECVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVLHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(22379..23491)
                     /gene="potF"
                     /locus_tag="ROI21_001282"
     CDS             complement(22379..23491)
                     /gene="potF"
                     /locus_tag="ROI21_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDX5110229.1"
                     /translation="MTALNKKWLSGLVTGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(23842..24318)
                     /locus_tag="ROI21_001283"
     CDS             complement(23842..24318)
                     /locus_tag="ROI21_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDX5110230.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFMRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(24406..25308)
                     /gene="rimK"
                     /locus_tag="ROI21_001284"
     CDS             complement(24406..25308)
                     /gene="rimK"
                     /locus_tag="ROI21_001284"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDX5110231.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(25369..26091)
                     /gene="nfsA"
                     /locus_tag="ROI21_001285"
     CDS             complement(25369..26091)
                     /gene="nfsA"
                     /locus_tag="ROI21_001285"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDX5110232.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAKAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDELAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(26075..26362)
                     /locus_tag="ROI21_001286"
     CDS             complement(26075..26362)
                     /locus_tag="ROI21_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDX5110233.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSGSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            26522..26779
                     /gene="grxA"
                     /locus_tag="ROI21_001287"
     CDS             26522..26779
                     /gene="grxA"
                     /locus_tag="ROI21_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDX5110234.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(26809..27186)
                     /locus_tag="ROI21_001288"
     CDS             complement(26809..27186)
                     /locus_tag="ROI21_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDX5110235.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            27456..29141
                     /locus_tag="ROI21_001289"
     CDS             27456..29141
                     /locus_tag="ROI21_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDX5110236.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMENRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(29316..29852)
                     /gene="rcdA"
                     /locus_tag="ROI21_001290"
     CDS             complement(29316..29852)
                     /gene="rcdA"
                     /locus_tag="ROI21_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDX5110237.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDATGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            29936..31144
                     /locus_tag="ROI21_001291"
     CDS             29936..31144
                     /locus_tag="ROI21_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5110238.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            31144..31959
                     /gene="ybjI"
                     /locus_tag="ROI21_001292"
     CDS             31144..31959
                     /gene="ybjI"
                     /locus_tag="ROI21_001292"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDX5110239.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWRIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            32044..32328
                     /locus_tag="ROI21_001293"
     CDS             32044..32328
                     /locus_tag="ROI21_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110240.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(32369..33601)
                     /gene="mdfA"
                     /locus_tag="ROI21_001294"
     CDS             complement(32369..33601)
                     /gene="mdfA"
                     /locus_tag="ROI21_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDX5110241.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            33886..34482
                     /gene="ybjG"
                     /locus_tag="ROI21_001295"
     CDS             33886..34482
                     /gene="ybjG"
                     /locus_tag="ROI21_001295"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDX5110242.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAI
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            34540..35298
                     /gene="deoR"
                     /locus_tag="ROI21_001296"
     CDS             34540..35298
                     /gene="deoR"
                     /locus_tag="ROI21_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDX5110243.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIMVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(35345..36547)
                     /gene="dacC"
                     /locus_tag="ROI21_001297"
     CDS             complement(35345..36547)
                     /gene="dacC"
                     /locus_tag="ROI21_001297"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDX5110244.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            36794..37420
                     /gene="gstB"
                     /locus_tag="ROI21_001298"
     CDS             36794..37420
                     /gene="gstB"
                     /locus_tag="ROI21_001298"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDX5110245.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWT
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(37417..38532)
                     /gene="yliI"
                     /locus_tag="ROI21_001299"
     CDS             complement(37417..38532)
                     /gene="yliI"
                     /locus_tag="ROI21_001299"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDX5110246.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPEVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(38643..39026)
                     /gene="bssR"
                     /locus_tag="ROI21_001300"
     CDS             complement(38643..39026)
                     /gene="bssR"
                     /locus_tag="ROI21_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDX5110247.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            39239..40564
                     /gene="rimO"
                     /locus_tag="ROI21_001301"
     CDS             39239..40564
                     /gene="rimO"
                     /locus_tag="ROI21_001301"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDX5110248.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            40790..41176
                     /locus_tag="ROI21_001302"
     CDS             40790..41176
                     /locus_tag="ROI21_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDX5110249.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            41166..41495
                     /locus_tag="ROI21_001303"
     CDS             41166..41495
                     /locus_tag="ROI21_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDX5110250.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(41565..42893)
                     /locus_tag="ROI21_001304"
     CDS             complement(41565..42893)
                     /locus_tag="ROI21_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDX5110251.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQIVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEFNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGVSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSLYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(42901..45249)
                     /locus_tag="ROI21_001305"
     CDS             complement(42901..45249)
                     /locus_tag="ROI21_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDX5110252.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMINDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKENSLCVEITETVIERINEHFYLNIEQLRKQGVQI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(45427..46338)
                     /gene="gsiD"
                     /locus_tag="ROI21_001306"
     CDS             complement(45427..46338)
                     /gene="gsiD"
                     /locus_tag="ROI21_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDX5110253.1"
                     /translation="MRLFNWRRQAALNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(46341..47261)
                     /gene="gsiC"
                     /locus_tag="ROI21_001307"
     CDS             complement(46341..47261)
                     /gene="gsiC"
                     /locus_tag="ROI21_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDX5110254.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(47279..48817)
                     /gene="gsiB"
                     /locus_tag="ROI21_001308"
     CDS             complement(47279..48817)
                     /gene="gsiB"
                     /locus_tag="ROI21_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDX5110255.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGHRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(48837..50708)
                     /gene="gsiA"
                     /locus_tag="ROI21_001309"
     CDS             complement(48837..50708)
                     /gene="gsiA"
                     /locus_tag="ROI21_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDX5110256.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAVVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCHPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLTGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHLYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(50695..51660)
                     /gene="iaaA"
                     /locus_tag="ROI21_001310"
     CDS             complement(50695..51660)
                     /gene="iaaA"
                     /locus_tag="ROI21_001310"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDX5110257.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAQLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            51864..53099
                     /gene="moeA"
                     /locus_tag="ROI21_001311"
     CDS             51864..53099
                     /gene="moeA"
                     /locus_tag="ROI21_001311"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDX5110258.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASPLKKSPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEAGEWVEVEPFNALFGGL"
     gene            53099..53848
                     /gene="moeB"
                     /locus_tag="ROI21_001312"
     CDS             53099..53848
                     /gene="moeB"
                     /locus_tag="ROI21_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDX5110259.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(53924..54586)
                     /gene="fsa"
                     /locus_tag="ROI21_001313"
     CDS             complement(53924..54586)
                     /gene="fsa"
                     /locus_tag="ROI21_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDX5110260.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMDGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            54717..55616
                     /locus_tag="ROI21_001314"
     CDS             54717..55616
                     /locus_tag="ROI21_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDX5110261.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPDVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEQPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            55622..>58035
                     /locus_tag="ROI21_001315"
     CDS             55622..>58035
                     /locus_tag="ROI21_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDX5110262.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVRKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIAR"
BASE COUNT        14480 a        15103 c        14207 g        14245 t
ORIGIN      
        1 gtttgtaatt aaagcgcaag aatatcttta aatgaaaatg aataccctac cgatccttat
       61 gcataacatt tccttttaat tgcaacttta ctcgtccttc cgctttcagt agtgtgtcat
      121 cgcgcatttc gacttcatct ttgcaaggac tgggttttca tgttttctgg gctgttaatc
      181 attctggttc ccctgattgt gggttacctc attccgcttc gccaaaaagc tgcattaaga
      241 gttattaatc agctattaag ttggatggtt taccttattc tcttttttat gggtatcagt
      301 ctggcgtttc tcgataacct cgccagtaac ctgttggcga ttctgcatta ttctgctgtc
      361 agtattaccg ttattttact gtgtaatatt gccgccctga tgtggctgga gcgcggtctg
      421 ccgtggcgca actaccacca gcaagaaaaa ctcccgtcgc gtattgcgat ggcgctggag
      481 tcgctaaaac tgtgcggcgt agtagtgatt ggttttgcca ttggcctgag tggactggct
      541 ttcttacaac acgcgaccga agccagtgaa tacaccttaa ttctactact tttcctcgtc
      601 ggtattcagt tgcgcaataa tggcatgacc ttaaagcaga ttgtccttaa tcgccgggga
      661 atgattgtcg ccgtggtggt ggttgccagt tcattaattg gtggtttaat taacgccttt
      721 attcttgatc ttcccatcaa taccgcgctg gcaatggcct ccggtttcgg ctggtattct
      781 ctttccggta ttttattgac cgaatctttt ggtccggtaa tcgggagcgc ggcgtttttt
      841 aatgatctgg cccgtgagct gattgctatt atgttgatcc ctgggctgat tcgccgcagc
      901 cgctcgactg cactgggctt atgcggtgcc acatcaatgg atttcaccct gcccgttctt
      961 caacgtactg gcgggctgga tatggtcccg gcggcaattg ttcacggttt tattcttagc
     1021 ctgttagtgc cgatcctcat cgcctttttc tctgcgtaat acctctctgg cggtagatcc
     1081 ctgccgccaa aattgcgcta aatcaatctc ccttaaagtt gcatgaaaaa tcccttttat
     1141 ccctgcgtta agcgtcttaa ccttaaacat gtatattaaa tataacttta aaaggtgtga
     1201 tcatgttttg tgtgcaatgt gaacaaacta tccgtactcc ggcaggaaac ggctgctcat
     1261 acgcgcaggg gatgtgtggt aaaacggcgg aaacctctga ccttcaggat ttactcatcg
     1321 cggcgctgca agggctttcg gcctgggcgg taaaagcgcg tgaatacggc atcatcaacc
     1381 acgatgtaga cagctttgcg ccacgcgcat ttttctcaac cctgaccaac gttaacttcg
     1441 attctccgcg tatagtcggc tacgctcgtg aagcgattgc cctgcgcgag gcgctgaaag
     1501 cacaatgcct ggctgtagat gccaacgccc gcgtcgataa cccgatggct gacctgcaac
     1561 tggtgagcga tgatctcggc gagctgcaac gtcaggcagc agaatttacc cctaacaaag
     1621 ataaagcggc gattggcgaa aacattctcg gcctgcgcct gctgtgcctg tatggcctga
     1681 aaggtgcggc ggcctatatg gaacacgcac acgttctcgg tcaatacgac aatgatattt
     1741 acgcccagta ccataaaatc atggcgtggc tggggacctg gcctgccgat atgaacgcgc
     1801 ttcttgagtg ttcaatggaa atcggccaga tgaacttcaa agtgatgagc attctggatg
     1861 caggcgaaac cagtaaatac ggtcacccga cgccaaccca ggtcaacgtc aaagcgacgg
     1921 cgggtaaatg cattctgatt tccggtcacg atctcaaaga tctctacaac ctgctggaac
     1981 agaccgaagg cacgggcgtt aatgtctaca cccacggcga aatgctgcct gcgcatggct
     2041 acccggagct gcgtaaattc aagcatctgg tcggtaacta cggcagcggc tggcagaatc
     2101 agcaagtgga gttcgctcgt ttccctggcc ccatcgtgat gacctcgaac tgcatcatcg
     2161 acccaaccgt aggcgcttat gacgatcgta tctggacccg cagcatcgtt ggctggccgg
     2221 gtgtgcgtca tctggatggc gaagatttct ctgcggtcat cgcccaggcg caacaaatgg
     2281 cgggcttccc gtacagcgaa attccgcacc tgatcaccgt gggctttggt cgtcaaacgc
     2341 tgcttggcgc agcggatacg ctgattgatc tggtaagccg tgaaaaactg cgtcatatct
     2401 tcctgcttgg cggctgtgac ggcgcacgcg gcgagcgtca ctacttcacc gatttcgcca
     2461 ccagcgtgcc ggatgactgt ctgatcctga ctctcgcctg tggtaaatat cgctttaaca
     2521 aactcgagtt tggcgatatc gaaggtctgc cgcgtctggt agatgcaggt caatgtaacg
     2581 atgcttactc agcgattatt ctggcggtaa cgctggcaga aaaactgggc tgcggtgtca
     2641 acgatctgcc gctgtcgctg gtgctctcgt ggtttgaaca gaaagcgatt gtcattctgc
     2701 tgacactgct ttctctgggt gtaaaaaata tcgtaaccgg cccgactgca cctggtttcc
     2761 tgacaccaga cctgctggcg gtgctgaacg agaaattcgg cctgcgttct atcaccactg
     2821 ttgaagaaga catgaagcaa ctgttgagcg cgtaaggagt ttgtcgatga cgatgccaac
     2881 gaatcaatgc ccatggcgga tgcaggttca tcacattacg caagaaacgc cggatgtgtg
     2941 gacgatttcc ctgatttgcc acgattacta cccatatcgc gccgggcaat atgcactggt
     3001 cagcgtgcgt aactcagcgg aaacgctgcg tgcttacacc atttcctcca cgccaggcgt
     3061 gagtgaatat atcaccctga ccgtgcggcg gattgatgac ggtgtcggct cccagtggct
     3121 gacgcgcgat gtaaaacgcg gtgattatct ctggctttcg gacgcgatgg gggaatttac
     3181 ctgcgacgat aaagcagaag ataaattcct gttgctggcg gcaggctgcg gcgtcacgcc
     3241 gattatgtcg atgcgtcgct ggctggcgaa gaaccgtccg caggccgatg tgcaggtgat
     3301 ctacaacgtg cgttcgccgc aggatgttat tttcgccgat gagtggcgca actatccggt
     3361 gacgctggtg gcagaaaata acgttaccga aggctttatc gctggtcgtc tcactcgcga
     3421 actgctgact cgcgttcctg acttagcttc acgtaccgtg atgacctgcg gcccggctcc
     3481 gtatatggat tgggtagagc aggaagtgaa agcgctcggc gtgacgcgtt tctttaaaga
     3541 gaaattcttc accccagtag cggaagcggc gaccagcggt ctgaaattca ccaaactgca
     3601 accggcacga gaattttacg ccccggttgg caccacgcta ctggaggcgc tggaaagcaa
     3661 taacgttccg gttgtcgccg cctgccgtgc aggtgtttgc ggctgctgta agacaaaagt
     3721 ggtttccggt gaatatacgg taagcagcac aatgacgctg accgacgccg aaatcgctga
     3781 aggttacgta ctggcctgct cctgccatcc gcagggagat ttggttctcg cataatcgcc
     3841 ttatgcccga tgatattcct ttcatcgggc tatttaaccg ttagtgcctc ctttttctcc
     3901 catcccttcc ccctccgtca gatgaactaa acttgtaacc gttatcacat tcaggagatg
     3961 gagaaccatg aaacaaacgg ttgcagctta tatcgccaaa acactcgaat cggcaggcgt
     4021 gaaacgcatc tggggagtca cgggcgactc tctgaacggt cttagtgaca gtcttaatcg
     4081 catgggcacc atcgagtgga tgtccacccg ccatgaagaa gtggcggcct ttgccgctgg
     4141 cgctgaagca caactttcag gagaactggc ggtctgtgcc ggatcgtgcg gccccggaaa
     4201 cctgcactta atcaacggcc tgtttgattg ccaccgcaat cacgttccgg tactggcgat
     4261 tgccgctcat attccctcca gcgaaattgg cagcggctat ttccaggaaa cccacccaca
     4321 agagctattc cgcgaatgta gtcactattg cgagctggtt tccagcccgg agcagatccc
     4381 acaagtgctg gcgattgcca tgcgcaaagc ggtgcttaac cgtggcgttt ccgttgtcgt
     4441 gttaccgggc gacgtggcgt taaaacctgc gccagaaggg gcaacctcgc actggtatca
     4501 tgcgccacag ccagtcgtga cgccggaaga agaagagtta cgcaaactgg cgcaactgct
     4561 gcgttattcc agcaatatcg ccctgatgtg tggcagcggc tgcgcggggg cgcataaaga
     4621 gttagttgag tttgccggga aaattaaagc gcctattgtt catgccctgc gcggtaaaga
     4681 acatgtcgaa tacgataatc cgtatgatgt cggaatgacg ggattaattg gcttctcgtc
     4741 aggtttccat accatgatga acgccgacac gttagtgcta ctcggtactc agttccccta
     4801 ccgcgccttc tacccgaccg atgccaaaat cattcagatt gatatcaacc cagccagcat
     4861 cggcgctcac agcaaggtgg atatggcact ggtcggcgat atcaagtcga ctctgcgtgc
     4921 attgctgcca ttggtggaag aaaaagccga tcgcaagttt ctggataaag cgctggaaga
     4981 ttaccgcgac gcccgcaaag ggctggacga tttagctaaa ccgagcgaga aagccattca
     5041 cccgcaatat ctggcgcagc aaattagtca ttttgccgcc gatgacgcca tctttacctg
     5101 tgacgttggt acgccaacgg tgtgggcggc acgttatctg aaaatgaacg gcaagcgtcg
     5161 tctgttaggt tcgtttaacc acggttcgat ggctaacgcc atgccgcagg cgttgggtgc
     5221 gcaggcaacc gagccggaac gtcaggtggt cgccatgtgc ggcgatggcg gttttagcat
     5281 gttgatgggc gatttcctct cagtagtgca gatgaaactg ccggtgaaaa ttatcgtctt
     5341 taataacagc gtgctgggct ttgtggcgat ggagatgaaa gccggaggct acctgacaga
     5401 cggtactgag ctgcacgaca ccaactttgc ccgcattgcc gaagcgtgcg gaattacggg
     5461 tattcgcgtg gaaaaagcct ctgaagtcga tgaagccctg caacgcgcct tttccatcga
     5521 cggtccggtg ctggtggatg tggtggtcgc caaagaagaa ttagccattc caccgcagat
     5581 caaactcgaa caggccaaag gtttcagcct gtatatgctg cgcgcaatca tcagcggacg
     5641 cggcgatgaa gtgatcgaac tggcgaaaac aaactggcta aggtaaaaag ggtggcattt
     5701 cccgtcataa taaggacatg ccatgattga tttacgcagt gataccgtta cccgaccgag
     5761 ccgcgccatg ctcgaagcga tgatggccgc cccggttggg gacgacgttt acggagacga
     5821 ccctaccgtt aatgctctgc aggactatgc agcagagctt tccggtaaag aagccgccat
     5881 ttttctgcct accggcactc aggccaacct ggtcgctctg ctcagtcact gtgaacgcgg
     5941 cgaagagtat attgtcggtc aggccgcgca taactatctg tttgaagccg gtggcgcggc
     6001 ggtgctgggc agtattcaac cgcaacccat cgacgcggct gccgacggca cgctaccgct
     6061 ggataaagtg gcgatgaaaa tcaaacccga cgatatccat ttcgcccgca ccaaattact
     6121 cagtctggaa aacacccaca acggcaaagt gctgccgcgg gaatacctga aagaagcatg
     6181 ggaatttact cgcgagcgca atctggcgct gcatgttgac ggtgcgcgca tctttaatgc
     6241 catcgtggct tatggctgcg aactaaaaga gatcacgcaa tattgtgatt cgttcaccat
     6301 ttgtctgtcg aaaggtcttg gtacgccagt cggttcgtta ctggtcggta accgtgacta
     6361 cattaaacgt gcaattcgct ggcggaaaat gacaggtggc gggatgcgcc agtccggcat
     6421 tctggcagcc gccggaatgt atgccctgaa aaataacgtc gcacgcttgc aggaagatca
     6481 tgacaatgcc gcctggatgg cggagcagtt gcgtgaagca ggcgcggatg tgatgcgtca
     6541 ggacaccaat atgctgtttg ttcgcgtcgg ggaagaaaat gctgccgcgt taggcgaata
     6601 catgaaagcg agaaacgtgc tgattaacgc ctcgccgatt gtccgcctgg tgacgcatct
     6661 cgacgtctcg cgcgagcaac tggctgaagt cgccgcccac tggcgcgcat tcctggcgcg
     6721 ttaaggaggg agacgtgccg caacgcattt tagttctcgg tgccagtggc tacattggtc
     6781 agcatctggt gcgaacactc agccagcagg ggcatcagat cctggcggcg gcacgtcatg
     6841 tcgacaggct tgcaaagctg caactggcaa atgtcagttg ccataaagtc gatctcagct
     6901 ggccggataa ccttccggcc ctgttgcagg atatcgatac ggtctatttt ctggtgcaca
     6961 gcatgggcga aggcggcgat tttatcgctc aggagcgcca ggtggctctc aacgtccgcg
     7021 atgcgctacg tgaagtccca gttaagcaat taatctttct cagttcgttg caggccccgc
     7081 cacatgagca atcggaccat ctgcgtgccc gtcaggctac ggcggatatt cttcgtgaag
     7141 cgggtgtccc ggtgaccgaa ttgcgtgccg gaataatcgt tggtgcaggt tcagcggcgt
     7201 tcgaagtcat gcgcgatatg gtctacaacc tgccagtctt aacgccgcca cgctgggtac
     7261 gttcacgcac cacgcccatc gcgctggaaa acttgctgca ctatctggtg gcgctgttag
     7321 atcatccggc cagcgaacat cgcatcttcg aagccgccgg accagaagtg ctcagttatc
     7381 agcaacagtt tgaacatttt atggcggtga gcggtaagcg ccgctggttg atccccatcc
     7441 cctttccgac ccgctggatt tcggtgtggt ttctcaatgt gattacttcc gtaccgccca
     7501 ccaccgccaa agcgttgatt caggggctga aacacgatct gctggcggat gacaccgcgt
     7561 tacgtgcact catcccacaa cggctgattg ctttcgatga cgcggtacgt cgcaccctga
     7621 aagaggaaga aaagctggtc aactccagcg actggggata cgacgctcag gcctttgccc
     7681 gctggcgacc ggagtacggt tatttcgcca aacaggcggg gtttaccgtt aaaacgtccg
     7741 ccagccttgc ggctttatgg caggtggtga atcaaatcgg cggtaaagag cgttatttct
     7801 ttggcaatat tttgtggcaa acccgggcgt tgatggaccg cgcgatcggt cataagctgg
     7861 cgaaaggccg cccggagcgt gaatatttgc agaccggcga tgcggtggat agctggaaag
     7921 tgattgtcgt tgaaccggaa aaacaactta cgctgttatt tggcatgaaa gcgccggggc
     7981 tgggacgact gtgttttacc ctggaagata aaggcgacta tcgtactatc gatgttcgcg
     8041 cattctggca tccgcacggt atgccggggc tgttttactg gttattgatg atccccgcgc
     8101 atctgtttat ttttcgcgga atggcaaaac gaatcgccag actggcagaa caaagcacag
     8161 attaatcgac aaaacctgcg ttattctttc atgtttccca ttgcatcatg ggggaaatca
     8221 cggaagaatg cgcacgtcat tttctcaaca acagtggatt gatatgaagg tactggttac
     8281 cggcgccacc agcggcttag gtcgaaacgc ggtagagttt ttatgccaga aaggcatcag
     8341 cgtgcgagcg accggtcgca acgaggcaat gggtaaattg ctggaaaaaa tgggcgcaga
     8401 gtttgttccg gcggatctga ccgagctggt ctcatcacaa gctaaagtga tgctcgcggg
     8461 cattgatacg ctgtggcact gctccagctt tacctcgccc tgggggacac aacaggcttt
     8521 cgatctggct aacgttcgcg ccactcgccg cctgggtgaa tgggctgtcg cctggggtgt
     8581 gcgtaacttt attcatatct cttccccctc cctgtacttc gattatcacc accatcgcga
     8641 tattaaagaa gattttcgcc ctcaccgctt tgccaacgag tttgcccgca gcaaagcagc
     8701 cagcgaagaa gtgatcaata tgctttcgca ggcgaatcca caaacgcgct ttactattct
     8761 gcgcccacaa agtctgttcg gaccgcacga taaagtcttt atcccccgtc tggcgcatat
     8821 gatgcaccac tacggcagca ttctgttacc gcacggcggc agtgcgctgg tcgatatgac
     8881 ctactatgaa aatgccgtgc acgccatgtg gctggcaagc caggaagcct gcgataagct
     8941 accttccggg cgtgtgtaca acatcaccaa cggcgagcat cgcacactgc gcagcatcgt
     9001 gcagaagctg atcgacgagt tgaatattga ctgtcgtatt cgttccgtcc cctacccgat
     9061 gctggatatg atcgcccgca gcatggagcg tttaggccgc aagtcagcaa aagagccgcc
     9121 gctgacccac tacggcgtct ccaagttgaa ttttgacttt acgctggata ttacgcgtgc
     9181 gcaggaagag ttaggttatc aaccagtcct caccctggat gaaggtatcg agaaaactgc
     9241 cgcctggcta cgcgaccacg gaaaactgcc gcgctaatcc tgcccgtatt tctccagcaa
     9301 cgcttcggca atcgcgtgag tttctgcatc cgcttcgccg ttgtataacg tcgggcggaa
     9361 atgcatctgg aacgccataa tcacgcgccg ctgctcgcgc ggtgtcatat caggtttaac
     9421 gtcataaccg tagcgcgcca aaagctccag caatgatgcg gtatctaccg gtgtgtgcgg
     9481 cgcgcgcccg gcaaggtaaa agttcacccg ttgcgcatcc ggccaggcac caatcccctg
     9541 ctgcgccagt tgttgccagg ggaataacgg ccccggatcg tctttgcgct gcggcgcaat
     9601 atccgcatgt gccactacgt tttccggctt gatgtgataa cgggcaataa tatctttcgc
     9661 cagcggaata agcgcctgaa tctgtgcctg ttcaaacggg gcaaaatatt tcactccggc
     9721 tgatttctgc cagccacggt tttccagctc aatgccaata gaggtgtcgt taaggcgcgt
     9781 tgccccgcgc caggcgctaa tccccgcatg ccaggccagc tcctgttccg gcaccagttg
     9841 ccagatgcgc ggtttaccgt tgtatcgcgg tggcacagca ggcaccagat aatgcgaact
     9901 gacctgctta tcggtcagtg tcgccagcga gctatcaaaa tcatctgcgg tgtagtggat
     9961 caccagcact ttaatgcgcg gatacgccgc ctgcgcctgg cgtcgggtat caagctgata
    10021 tccctctttc tcgacaatgc ctttttcgcc tgcacaccct gccagtaaca gagcgacagc
    10081 gatcagccag aagactcttc tcatcgacgc gttttcaccg ccgtaccgct gacgctaacc
    10141 atcagcatac tgccgttttg cccgaccgtt tcgtagtcga tatcaatacc gactacggca
    10201 tcggccccca gtgcccgcgc ctgatcgccg agctcctcaa aggcgatctc ccgcgcttta
    10261 cgcaattctt tttcataagc cccggagcgt ccgccaacga tatcgcggat accggcaaag
    10321 aaatcacgga aaatattggc acctaaaatc gcttcgccgg tcaccacacc gcaatattcg
    10381 acgatggtct ggccttccag agttggggtt gtagaaaatt gcatgatcga tctccttgtt
    10441 tgttaagcct tcgatctcta aagcattatc ggactgatat gccatgttgg gaagaaggtt
    10501 ttgatcagtt taggaaatca tctcattgtg tcaaaaacag gtacataagc ctgtacaatt
    10561 atctgtttcg taaccggata ttggttttat tatggagcac cgcttacctg tcgtattatg
    10621 tagtaccagc aacattaatg agtgggaaga gtcaacttat attgtaaatg attcctaaag
    10681 tcttccccat cttgcgcctg aggtatatat ctttggattg ctttttggtc agccgccgca
    10741 aaacaagaaa gtaataagat ctcaatgctt ttgtcattaa tataagcgaa gatcattctt
    10801 gcaaattcgt atataaacgc ccgatcctga gcgcttaata gtttaataca atatttattt
    10861 ttaagagaat aaagtatttc aaggaaagca tccgcagcaa ccttcacgtt attaccggcg
    10921 atcatattat atatatctct tgcacagata gagtacagag atagtaaaca gccatacaaa
    10981 taatcattta ttaattcttt tgtccatttt actggcaacc atggttttat cttgacacca
    11041 tgcatactac agtcatgaaa aagacattta ctgaaattaa ctcttatcat atcaacattg
    11101 aaaaaatggc agttaataaa ttttaaagaa tgagtattca cattgttaat tttaacagtt
    11161 ctaaacgaaa cgtttttgaa tacattgttt actatattgc tatttgttat tttaacatca
    11221 cctaaactgc cgttttcaaa acaacatttt ttcaatatgc tttcattcac aacagtgtgt
    11281 gataaataga gattacttat gttttgcttt attaatgcca cgtcattaaa atgactatat
    11341 ttaataaagc aaccatcatt gagtagtgtt ttaagattaa ctaaattagt tgcatcttca
    11401 cgtaccatcg tggcaactgg aacaggcaac gacatgaaaa cttcttcatt caccatcaag
    11461 tggatcatgc attgcgttcc ctttgtcata tccacactta cgctacaaat atcgttgtaa
    11521 tgcgaagagt gcatttcagg aaacaaatta aacacggtat cttgaaaggc actaaatcgt
    11581 gttaataaat cctgataatg aacaggtttt gtttcaaaat tatcatcagc aaatccaagc
    11641 actattgcat gctctacatc taacgcagtt tcaaatgact cttcttctgt aaaatcatta
    11701 tttaaaacct cacctgacgt attatcactg tcataaaaag tgctcatcaa tgcgatattg
    11761 tttgttacag cgtcttttgc gatattctca ctaattgcat atcgcctgct ataatcataa
    11821 aaatgaataa acgcatcctt tataaaggat atatattgct ctttgttaaa gtcaatacga
    11881 ctgtataatt tttcaaaaat cacattcgca atcttactac ccatagtatg cctgtataat
    11941 aatgggtgcg attcaatcgt aaaatacttg tctaattgat gttgtaattt attctcatag
    12001 tccagtggga aaagaaattt ttgtctttca agtttcccat tcgatatatt acgtattcca
    12061 atggtaattc tttcattacc cacttttatt tcaacttcgt tatcttctga tttgccaaga
    12121 tattttttaa catcaatctc gaaagcatta ctaaaaaata tattaaattg ataattcgct
    12181 gatggaatat tttccgctag caactcatca ttttttatga gatcactatc agcatttatt
    12241 gctaatatca gatgcaagta gatggtgtcc catgtagaca actcaccctt acgactggaa
    12301 tttaatatcg cgaaaaattc cctcagtgaa tcgaggaaat tatcactttg attcagttgt
    12361 gtgcctggaa atgatgatgc ttgatcagat atattgttta tcatattaaa aatcacccaa
    12421 gggaaaagat attcctctga actcctcgtg ctttattatt ataaaagcac aacgcattta
    12481 tactcatatt aattaacgaa acactgatac agtttgattt attcttcgct caaactcatt
    12541 agcttaaatt ctttacccac aactgtagca tatataatat tatcaaaagt caaagctggg
    12601 actatcaaat ttcgctcatt ttcaaaaata caatgtaatt ttagcaatca tcttaatcgc
    12661 aggatctctt attgcatgtg aaaaattctg taatgattag acaataaccg cgttcattta
    12721 tcacgcgtta taaatgtgaa actggcttct tgcagatgct cttaacctct acaggaggat
    12781 ttatcccgcc atttcagaac tatcatattt accttaaatg atcatttcag ccatataaca
    12841 cgctcaacct cacaaatggt ttacactgtc tattgtttgg gtaatttaag taagtcagct
    12901 acgagcaacc ttgagtgggt agccagcaat ggactcaaac ccaatcaggt gttcagattg
    12961 tttcagaatt gtattaaaaa caatgtatta aataatctga taacgtttgg tcgtagtgtg
    13021 acgcagacag caagctaata aggaaatgat tatgcgctac cgttcactat ctctacttat
    13081 tccatgtgca ctgctgctga gtgcctgtac caccgtcacg ccagcttata aagataacgg
    13141 cccccgcacc ggttcctgtg tccagggtgg ccctgacagc gtggctcaac aattctatga
    13201 ctaccgcatt caacatcgca gcaatgacat taccgccctg cgcccctatt tgagtgacaa
    13261 actggcaacc ctacttagcg atgccagccg caataacagt catcgtgaat tgttaagcag
    13321 cgatccgttc tccagtcgaa ccaccttgcc agacagcgcc cacgttgcca gtgcatcgac
    13381 tatccctaat cgtgatgccc gtaacattcc attgcgcgtg gatctcaaac agggtgacca
    13441 gggctggcag gatgaagtgt taatgattca ggaaggccag tgttgggtca tcgacgatgt
    13501 gcgctactta ggcggtagcg ttcatgccac agcgggtacg ctccgtcagt ccatcgagaa
    13561 tcgctaacgc cttgatcaac caataaaccc cgtaaattgt ccggcatttc gtgaggaatg
    13621 ccgacattta tgctcgccga ccaccgcccc cgttattttg tgctatgttt attgaataat
    13681 gcgctttgct tttaactttt aaagcagaaa tattgcataa ttattctgtc aaaggtacta
    13741 tcttcgacct caatagctat cagactgcca gtatacgagt gtcaatgagt attcaattaa
    13801 acggcattaa ttgcttctac ggcgcgcatc aggcgctgtt cgatatcacg ctggattgcc
    13861 cacagggcga aacgctggtg ttacttggcc ccagcggcgc gggtaaaagc tcgctgctgc
    13921 gtgtactcaa tctgcttgag atgccgcgct ccggtacgct caacattgca ggcaaccatt
    13981 tcgatttcac caaaacaccc tctgacaaag cgattcgcga tttgcgtcgt aacgttggca
    14041 tggtgtttca gcaatacaac ctgtggccgc atctgaccgt gcagcaaaac ctgattgaag
    14101 cgccctgccg tgtactgggg ttgagcaaag atcaggcgct gacccgtgca gaaaaactgc
    14161 tggaacgtct gcgtctcaaa ccttatagcg atcgttaccc gctgcatctt tctggtggtc
    14221 agcagcagcg tgttgctatt gcccgtgcgt tgatgatgga accgcaggta ctgctgttcg
    14281 atgaaccgac cgccgcactg gacccggaaa ttacggcaca aatcgtcagc atcattcgtg
    14341 agttggcaga aacgaatatt acccaggtga tcgtcaccca cgaagttgaa gtggcgcgta
    14401 aaaccgccag ccgagtagtg tatatggaaa acggtcatat agtagaacaa ggcgacgcga
    14461 gctgctttac cgagccgcaa accgaagcat ttaaaaacta tctctctcac taatattcgg
    14521 gataacaaca atgaaaaaag ttctgattgc cgcgttaatt gcaggtttta gtctttccgc
    14581 cacagctgcc gaaactattc gttttgctac cgaagcctcc tatcctccgt ttgaatcgat
    14641 tgatgcaaac aaccagatcg ttggttttga cgtcgacctg gcacaggcac tgtgtaaaga
    14701 gattgatgca acctgtactt tctctaacca ggcgtttgac agcctgatcc caagcctgaa
    14761 attccgtcgc gtagaagcag tgatggcggg catggatatc actccggagc gtgaaaagca
    14821 ggtgctgttt accacgccgt actatgacaa ctctgccctg tttgtgggtc agcaaggcaa
    14881 atacaccagt gttgatcagc tgaaaggcaa aaaagtcggc gttcagaacg ggacgacaca
    14941 ccagaagttc attatggata agcacccgga aatcaccacc gttccgtatg acagctacca
    15001 gaacgcaaaa ctggatctgc aaaacggtcg tatcgacagc gtattcggtg acactgcagt
    15061 ggtcaccgag tggctgaaag acaacccgaa actggcggca gtgggcgata aagtgaccga
    15121 taaagattat ttcggtactg gcctcggcat cgcggtacgt cagggcaaca ctgaactgca
    15181 gcagaaactc aacactgcgc tggaaaaagt gaagaaagat ggcacttacg aaaccatcta
    15241 taacaaatgg ttccagaagt aattcctgat gaatgaattt tttcctttag caagcgccgc
    15301 cgggatgacc gtcggccttg ccgtttgtgc attgattgtc gggctggcgc tggcgatgtt
    15361 ctttgcggta tgggagtctg caaaatggcg tcctgtcgcg tgggcaggtt cagcgctggt
    15421 aaccattctg cgtggcctgc cagaaattct ggtggtgctg tttatctatt ttggctcctc
    15481 gcagctgctg ctgacgcttt cggatggctt cactatcaat cttgggttcg tgcagatccc
    15541 ggtgcagatg gacattgaga acttcgacgt tagcccgttc ctttgtggtg tcatcgctct
    15601 gtcgctgctg tatgccgcct atgcctcgca aacgctgcgc ggcgcgttga aagcggtgcc
    15661 gatgggtcag tgggaatccg gccaggcgct ggggctgtcg aaatcggcta tctttttccg
    15721 tctggtgatg ccgcagatgt ggcgtcatgc gctgcctggc ctcggtaacc agtggctggt
    15781 gctgctgaaa gataccgcgc tggtcagttt gattagtgtg aatgatttaa tgctacaaac
    15841 caaaagcatt gctactcgta cccaggaacc gtttacctgg tacattgtgg cggcggcgat
    15901 ttacctggtg atcaccctgc tcagtcagta cattctcaaa cgcattgacc tgcgcgcgac
    15961 acgttttgag cggaggccca gctaatgttt gagtatttac ccgaactgat gaaagggctg
    16021 cacaccagcc tgacgctaac cgttgcctcg ctgattgtgg cactgattct ggcattgatt
    16081 tttaccatca tcctgacgct gaaaacgccg gttctggtgt ggctggtgcg gggttatatc
    16141 acgctgttta ccggtacgcc gctgctggtg cagatcttcc tgatttatta cgggccgggc
    16201 cagttcccga cgttgcagga gtatccggca ctgtggcatt tgctgtcaga accgtggctg
    16261 tgtgcgctga ttgcgttgtc gctgaacagt gcagcgtata ccacgcagct gttttacggt
    16321 gccattcgtg cgatcccgga aggtcagtgg cagtcctgta gcgctctggg aatgagcaaa
    16381 aaagatacgc tggcgatcct gctgccgtat gcctttaaac gctcgctctc ttcttattcc
    16441 aacgaagtgg tgctggtgtt caaaagtacc tctctggctt acaccattac gctgatggaa
    16501 gtgatgggat atagccagtt gttgtacgga cgcacctacg atgtcatggt gttcggtgcg
    16561 gcagggatta tttacctggc cgtcaacggc ctgctgacgc tgatgatgcg tctgatcgag
    16621 cgcaaagcac tggcattcga acggcggaat taatgtagct gcgtgctgat gccggatgcg
    16681 tcgtggacgt cttacccgac ctgcatagtg cataaattct tataatgaag acggtcaacc
    16741 cactaagttg tccgtctttt tttattttat ataaattatt taatcatatt tattgcatat
    16801 aaattcactt gatggcattg ttatcccatg ccgcagacac ggccaaaaat cataagattg
    16861 acagacggga gttccatcat gaaaaagtta gttcttgccg ctttacttgc ttcctttact
    16921 ttcggtgctt ctgccgcaga gaaaatcaat tttggcgttt cagccaccta tccacccttt
    16981 gaatctatag gtgctaataa tgagattgtc ggctttgata tcgacctggc aaaagccttg
    17041 tgcaaacaaa tgcaggcaga atgtactttt acgaatcacg ctttcgacag cctgatcccg
    17101 tcactgaaat tcagaaaata tgacgccgta atctccggta tggatatcac cccggagcgt
    17161 agcaaacagg tatcgtttac cacgccctac tatgaaaact cagccgtcgt gattgccaaa
    17221 aaagatacct acaaaacgtt tgccgatctg aaaggcaaac gtattgggat ggaaaacggc
    17281 accacgcacc agaaatatat tcaggatcag cacccggaag tgaaaactgt ctcctatgac
    17341 agttatcaga atgcctttat cgatctgaaa aatggccgta ttgatggggt atttggtgac
    17401 accgcggtgg ttaatgaatg gctgaaaacc aacccgcaac tgggcgttgc gactgaaaaa
    17461 gtgaccgatc cgcactactt cggcaccggc ctgggtatcg ctgtacgtcc ggataacaaa
    17521 gctctgctgg aaaaactgaa taatgcgctg gcagcaatta aagctgacgg tacatatcag
    17581 aaaatcaatg accagtggtt cccgcagtaa gattcagaaa gccgcacctg cggctttttt
    17641 attgctgaac cagcagtgtc agcacttcgt aatgcgcagt gtgcgggaac atatcaaaaa
    17701 gctgtacccg ttcaatacgg taacctggca gttcgcggat atctttcgcc atggtttggg
    17761 cgttacagct ggagtagatg ataaaacgcg gtgccatcgt tgagagataa tcacatagcg
    17821 gtttaccaat gccacgtcgc ggcgggttaa ccagcaccag ctcaggcact tccccctggg
    17881 cggtggcaaa ctgagtggag tccagcgcct gaaattgcaa acgcgttaag cccagttcag
    17941 cagctgactg attcgcacag gcaatggcct ctggtgcaat ttcgatcccg gttaactgca
    18001 tgtcaggcgt cgcgcagtgt aaaccaaagc cccccacgcc gcagaagaga tcccacatat
    18061 ggtttaccgg tagttgccgc acccagtcgc gcgcggtagc gtaaagctgg ctggcgaccg
    18121 ccggattggt ctggaagaaa ctttgcggac ggatccacag cggtacgtcg ttaaaacgct
    18181 ctgccagcgc ctgttgttcg gtcaggtaga tctccgtctc cccttccatg atcgccatat
    18241 gtaccggctg aatattgacg gtaataactt ttagctgcgg cagttgttcc tgtaaccacg
    18301 gcagcgcctt acgcagttgc gccagtttgg tttcagaacg cagtacaaag cgcagcatca
    18361 taccgccatc gctctggctt tcggtcagca gaatgtattt cagttcgcca cgcttacgcg
    18421 ctacgttgta gggtgtcagc cccgcacgag cgataaacgg ttttagcacc gcaaaaacgg
    18481 gcgcaaatga ggcaggataa agcgggcagt cacaaaggtc ttccggtgtg ccgtctcgat
    18541 gcagcatacc gagcagtggt ttttcaacgc taccactcac caccattttg gctttattac
    18601 gaaacccttg ttccgggcct gacaccggtg cgcaccattc ctcaaccgga aagtcggcga
    18661 gcagattttt aagatcggcg gttttagcgg agagttgctc tggaatcggc tgcgttatcc
    18721 actgacagga acgacagcga cccgcgtcgt aaagtgcgca ctgcatacat tgaccttcac
    18781 atcatcaggg gcgacgatta tacacattat tgcaactgga agaaccgccg actggtcgag
    18841 ggaacgaaca gcagcatcag gatgagcata tccggcagct tctgcagcat caggctatgg
    18901 aagatttcac gttttgattc accgggaatg ctgaacagct ccggataacc ataccccagc
    18961 gaagctgccc acaagtaact tgcggccgtg atttgagtca acagatacag ccagcgcgcc
    19021 cagcgacgcc ctttcaccag tgaaaacgca caccaaatct caataaaaac cagcaccaga
    19081 ctgcttaaaa agaccagcgt taaactccag gtttgtacgc tgcgatgaat gaactcgcca
    19141 ataccgcgca cgcccagcgt attgaaaatc atcagcacgt cgaggccacg gatcataata
    19201 atggcgagcg ccgccacctg caccagcgca ggcacattca ggcgagcatg agatgaagat
    19261 gttttcttaa aaaatcccaa cgtttcgtct tccatgaaaa caatgccgcg acatgcgcgg
    19321 cattatgtag ccaggttggc aaattttagt gtcttcagcc acgtcttgca cgctggatgt
    19381 cgcgtatccg ctgtttttct gcgcgcgcca tcagatacca ggcgataaat ccgacaattc
    19441 cgaccgcacc gaggatcaat gttgccaggg cgttgatttc cggattcacc cccatccgca
    19501 cgctggaaaa gaccagcatc ggtaacgtgg tggctcccgg cccggaaaca aagctggcga
    19561 tcaccagatc atcaagcgac aaagtaaaag ccagtaacca accagaaatg atcgcgggca
    19621 tgatcatcgg tagcgtaatg acgaaaaaca ctttcagcgg cgtcgcaccg agatccatcg
    19681 ctgcttcttc tatcgagcta tccagttccc gcagacgcga cgaaataacg actgccacat
    19741 aagccgtaca gaaagtgaca tgcgccagcc agatggtgag cataccgcgg tccgcaggcc
    19801 agccaatggc atgagcaagc gcgacgaata acaacaatag cgacaagccc gtgatgacat
    19861 ctggcatcac cagcggcgcg gtgatcataa aggcaaagcc atttgatccg cgaaacctgc
    19921 caaagcgcac cagcacgacc gccgcaatcg tcccgaggat cgccgccgcc gtcgccgcac
    19981 aggccgcaat tgtcaggctt aaaccaaccg cactcatcat tgcgtcatcg cgcaataact
    20041 caccatacca gcgtgttgac cagccggccc acaccgtcac cagcttcgag ctgttgaatg
    20101 aatagatcac cagcatcagc attggcgcgt agagaaaggt gaagcccagc agcaaaatca
    20161 caatccgcca gggcgaacga actaccggta aattattcat ccgtgttctc ccacgctttt
    20221 ttgctgatgc ttgtggaacc acattatcgg cacaattagc agcagcaaca tgatgatcgc
    20281 taccgctgag gccaccggcc agtcgcggtt gttaaagaac tcctgccaca gcacgcgccc
    20341 gatcatgatg ctgtccgggc caccgagcag ttcagggatc acaaactcgc ccacagccgg
    20401 gataaacacc agcatcgatc cggcaataat cccgcctttg gtcagcggca cgatcacagt
    20461 aaagaacgtt ttcagcggtc gtgcaccgag atccagtgcc gcttccacca gcgaataatc
    20521 aatacgaatc aaggcggtgt agatcggcag aaccataaac ggcacgtagg cataaacaat
    20581 gccgatataa acggccagat tggtatgcag aatggtcagc ggctggtcga taacccccag
    20641 ccacagcaga aaattattca gtacgccgtt attttttaat attcccatcc aggcataaac
    20701 gcgaatcaga aacgaggtcc acgacggcag gatcaccagt agcaataaga tattacgggt
    20761 cgaaggctta ctgtgcgcca ccgcccacgc cagcggatag ccgatcagta aacagcaaat
    20821 agtcgaaatc gctgccacct gtagcgactg gagataagca tcgaaataga gcggatcgtc
    20881 ggtcagttgc aggaaattac cgagattaag agtgatggaa agttgcccgt cagcccactc
    20941 catcagttcg gtataaggtg gaatagcgcg cgccatctcc gccaggctta ttttaaagac
    21001 aatcagaaat ggcagcagaa acagcaggat tagccagata tacggcagcg caatcatcag
    21061 tttgcgccca tgcttcattt gcagctgcga cagccacagc ttaaagggtt ttggctgggc
    21121 agcaggttca agtgtactca tctttgctcc ttaaaccgtc agcaccacac agctgtccac
    21181 ttcccagcac aaacgcactt catcgcccca ggtcggtaac cctttacgat gacgatgggc
    21241 gttttgtagc tgggcgctga tcatctgccc acttttcaga cgaacgtgat acaccgaaag
    21301 atcgccgaga taggcaatgt gtagcacctc ccccaccgcg aagttacaac cattggcggg
    21361 cggctcttcg caaagcatga ttttttccgg acgcagtgct acgtgtaccg gcacgttatc
    21421 gaccaccgaa gcatctacat cgactttcag tggatgcacc agccccggcg aataaagcac
    21481 cagaccatct tcctggcgct ctttgagtac acattcaaag acattaaccg agccaataaa
    21541 ttcggcgctg tagcgggtgg tcggatgctc atagatctct tccggttcgc caatctggac
    21601 aaatttccca cgattcataa tggcgatgcg ccccgccatg gtcatcgcct cttcctgatc
    21661 gtgggtgacc atcacacaag tcacaccgac acgctccaga atatccacca cttcaagctg
    21721 catccggtca cgcagctttt tatccagcgc gcccatcggc tcatcgagca gtaatagttt
    21781 cgggcgcttg gcaaggcttc gggccagggc cacacgctgt cgctgaccac cggaaagctg
    21841 atgcggtttg cgtttggcga actcctgcat atgcaccagg ccgagcatct cattgacccg
    21901 gctggcaatt tccgctttcg gcaatttgtc ctgtttcagg ccaaaagcga tgttctgttc
    21961 cacggtcata tgcggaaaca gcgcgtaaga ctgaaacatc atattgatgg ggcgcaggta
    22021 aggcggaacc tgtgacaaat cgacgccatc gagcataatc tgcccggcag aaggttgttc
    22081 gaaacctgcc agcatacgca gcagcgtgga cttgccacag ccggatgcgc ccagcagcgc
    22141 gaagatttcg cctttataga tggtcaggct gacatcatcg accgcatgct gaccatcgta
    22201 agatttagtc agattacgga tttctaatag cggcgtcagc gccttacggg ttttcgcctg
    22261 cgggcgaggg atagcgtcat tcacggctgc tctccggcat tgaatgtgtg caagcgcacc
    22321 ataatggtgc gaattgccgg cgggtgtggt gcgcccgtcc ggcatctacg actgcggatt
    22381 attttccgct cttcactttg gtccacgcgc gggtgcgcac acggtctatt tttggatcct
    22441 gcactttcag agtgaacaac ttcgcacgaa cgtccgccgg aggataaatc cctggattat
    22501 cacgcacttc cgcactcacc agcggcgtag ctgctttatt ggcgttggca tagaacacat
    22561 ggtcagagat atgtgccacc acatccgggc gcagcaggta attcaggaac tgataggctt
    22621 cgtctttatt tttggcatcc gcaggcatgg cgaagacatc aaagaacgcc atcgcccctt
    22681 cttttggaat cgagaacgag acattcacac cattcttcgc ttccttcgcg cggtttgacg
    22741 cctgccagac atcgcctgcc cagccgatag cgacgcagat atcgccgttt gccaggtcgt
    22801 taatgtattg agatgaatgg aaataacgaa tgttcgggcg cagctttaac agcagatcgg
    22861 ttgccggtcc ggtgtaatca tctgctttag tgctgttggg atctttaccg agataattca
    22921 gcacggtagc aaacacttct tccggtgcat caaggaaaga gacgccgcag cttttcagtt
    22981 tttccagatt ttcaggcttg aggatcaagt cccagctatc gacgggcgcg ttttcgccca
    23041 gcaccgcttt aactttatca acgttatagc caatcccggt cgtcgcccac atatagggca
    23101 tagcaaattt attgtcggga tcgtgtttgg caaccaactt cagcagctcc ggatcgagat
    23161 tcttccactc cggcaatttg cttttatcca gcggctggaa aactcccgca gtcaactggc
    23221 gttcaagaaa gcttgctgac ggcaccacca ggtcaaagcc ggtgctaccc gccattaatt
    23281 tgccttccag tacttcgtta gagtcgaaaa catcgtagac gactttaata ccggtttctt
    23341 tttcaaaatt ggccaccgtg tccggggcga tataatcaga ccagttataa atgtgaagtg
    23401 ttttttgttc agccgcgagc gtgccgacag agacggccat cagagcaccc gtaaccagac
    23461 ccgatagcca ttttttattt aaggcggtca tatctctttc cttctgaaag ttcgttaaca
    23521 aacgaacaaa agttgtcaca ctgaaagata tgcaataaac gtgcatattt tttatcggcg
    23581 cggtgtaaga aaagagaact ctctcccggc cagcactgct cgctatataa aagcatcgca
    23641 agaataactt tagccagggt ttgagactat agctcggatt aaaaaacggt atttaacaaa
    23701 tttttatgcg tatttgctct atgtgataaa gccaaatcgg cacaagagaa gtgaaataat
    23761 ctttaagaaa aggttaaaaa cagcagataa tttttcagca cgcagtcact gcggcagcga
    23821 ctgcgtactc gaaaggagtt atcagtggag gaaatagttt tcggtaacgt tgttttgccc
    23881 ttcatcgtca gtcggtaaca gcagttgatg ggcattagct tcaagaatga ccatcgaaat
    23941 ctgctcttcg ctctgacgca tgaaccatgc aaactgctca taggttacgc cctgcataac
    24001 ggataaagac tggcaaacca ccagctttgg cagattatca tcctgcatat caagaaatgc
    24061 tttcacggtc agcgaactgg cattgatggc agataaatct gccgccaggg gcaatacggc
    24121 tgaaggtcgg acttccgcca tggcagaaaa caggatcgtg ttatcgatca gatcgatttt
    24181 ggcatcaaag acaccgtcga aattctgcat atggggcaga tgcagagcct gacagttatc
    24241 acattcaaaa aaactcatcc ccaggtcatc gagccattga cgcagcgtat ccagaccagg
    24301 aacgaccagc gatgtcataa ttttgtacaa cctctttcga taaaaagacc ggcacagctt
    24361 acgcaaaaag cgcaggcaaa accatgatca gtaatgtgat tgcgactaac cacccgtttt
    24421 caggcaatat tccgtcgtag cgtggcgttc gatccagcgg atcattttac ccgcgatgtc
    24481 gataccggtg gttttttcta ttccttccag ccccggcgac gcattcactt ccatcaccaa
    24541 cggcccgcga ttagcacgca aaatatctac accagcaaca tccagcgcca tcgttcgcgc
    24601 ggcttttatc gcgatttcac gctcctgtgg tgtgatactg gcgacgcttg ccgcgccgcc
    24661 acgatgcaaa ttggaacgaa aatcgccctc tttcgcccgc cgttcaatcg cagcgaccac
    24721 ttcatcgcca acaaccagac agcggatatc gcacccttgc gcctctttga tatattcctg
    24781 caccagaata tgcgcgttca gaccgcggaa agcgtcaatc acgctttcgg ccgcctgacg
    24841 cgtctccgcc agcacgacac caattccctg cgtgccttca accaacttga ccaccagcgg
    24901 cgcaccaccg accatgtcga ttaaatcgct ggtatcatcc ggcgaatgcg caatgcccgt
    24961 gacaggcagg tcgatgccct gacgcgccag cagttgcatg gaacgcaatt tgtcacgcgc
    25021 ccgggcaatg gcgaccgact cattgagcgg atagctcccc agcatctcga actggcgcag
    25081 tgccgccgtc ccataaaagg taatggcggt gccaatacgc gggatcactg cgtcaaaatg
    25141 gggtaacttg cggcctttgt agtgaataga agacgccgca ggatttatgt tcatgtagca
    25201 agaaagcggg tcaagaattt caaccaggtg accgcgctgt atagcggctt cacgcagccg
    25261 cttacacgaa tagagcgttc catcccggga caatatggca attttcaccc tgcacctctc
    25321 tgtcagacct ggtcaaaagc ccgcgtatca tacatcggcg tggcggtttt agcgcgttgc
    25381 ccaaccctgt ttgtgcaaat aatccagaat aaatgggcgg ctttctttaa tgattgttcg
    25441 gcggatatga tcgctccagg tatcccggcg attattgctg ccacgtgtga ggtaatattc
    25501 cgccagttgc tcgtcatact gcgccagttc gtctttatcc agcggttgat agctgttttc
    25561 atgcaccaaa atggaggccg gtaaacgcgg cttaagatct ggattatccg caggccagcc
    25621 aaggcacagc ccaaacagcg gcagaacatg ctgcggtaat ttcagaagtt ccgtcaccgc
    25681 ttcaatatta ttgcgcaggc cgccgatata taccccaccc aatcccagcg attccgctgc
    25741 ggttaatgca ttctgcgcca tcattgccgt atcaacgaca cctagcaaca gttgttccgc
    25801 caggccaagt tgtgcatccg gacagatctg taaatggcga ttaaagtcgg cacagaacac
    25861 ccaaaactcc gccgctttcg ctacgtgttt ttgcccgccg gtcagcgtca ctaattcatc
    25921 acgtaacgct ttgtcggtaa tgcgaataat gctactgcac tgcaaaaaac tggaactaga
    25981 cgtcgcacgg gcgctgttaa taatcgcctc acgctgcgct tcggaaatgg gttcatcagt
    26041 gaaatggcga atggagcgat ggccacaaat aagttcaatg gttggcgtca ttatcttttt
    26101 ctctttctga acgtgaatat tgcggtggac ggttcatcag ctgtggggca agacgttttg
    26161 ccacctgaag aataaccacc accgcagcgg gaagcatgag caaaacaccg agaaaaatca
    26221 tcagaatctg cacttctggc cgagaaaatg gctcaggcag cgacagggag ccgcttaccg
    26281 acagcagcgc caccgccagc aacatcattc cgataaattc cagtatcaac acgcttttag
    26341 gcaatttacc gatcgcgcgc atacgcttcc ctctgcaaag tgagccttca gtctaaaact
    26401 tttcactgta ttgtgtttaa cagttatagc ttttagcaat taatgcaaca ggttaaacct
    26461 actttcagcg aatacatttt agcgtgatca ttacaggcat aaatctatga ggagagaaat
    26521 aatgcaaacc gttatttttg gtcgtccggg ttgcccttac tgtgtgcgtg caaaagatct
    26581 ggctgagaaa ttgagcaatg aacgcgatga ttttcagtat cagtatgtag atattcgtgc
    26641 ggaagggatc actaaagaag atctacaaca aaaggcaggt aaacccgtag aaaccgtgcc
    26701 gcagattttt gtcgatcagc aacatatcgg cggctatacc gattttgctg catgggtgaa
    26761 agagaatctg gacgcctgat cgtctgacaa gccctcgcgt tgagggcttt attgattttt
    26821 tctgtgctgt ggtttaaaca aactactgat aaataagaaa cacaatgccc ccagcgcaca
    26881 ccagaacacc gcacttaata accatgccag ctcttgccag aatgagcgcg tcggtgaaaa
    26941 aaacagccgc ataataagca tggaacaggg tgccgccagc attgcgccaa acagaggttt
    27001 caggacttct ctacgctgtg aaaagaagct ggcaactgct ccaggaagaa tgaaaaacag
    27061 caagccgatt tcaggatgcc cggcagcccg aaaagcgcct ttcatgtgcg tcgccagaaa
    27121 aaggcacacc acaatgaaga ggacaaaaca gcagattgcc cccgcccaac gttgtttatg
    27181 tttcactcgt tcctcctgac actgcgtcta tcgaacacat ttttcgccag tgtggcgttc
    27241 agtaagataa agccgcttcg cattccatgc taatataggc caacgcaatt catatagccg
    27301 ttgataccta atgtgattac actagtaaaa tatattgtta ctttactatc gtttaggtgc
    27361 gctgaatgaa tctgcgccct gaattctggt aaaaaacatt atcgtaaatt accatttctt
    27421 tcaacagctt actagtaaac aagaagttag cctccgtgaa tataaacgtc gccgaattgt
    27481 taaatgggaa ttacattctg ttattatttg tggtcctcgc gcttgggcta tgtctcggga
    27541 aattacgact tggttcgatc caactgggta attccattgg cgttttagtc gtatcgctgt
    27601 tattaggcca acaacatttc agcattaaca ccgacgcgct taatcttggc tttatgctgt
    27661 ttattttctg cgtcggggtc gaagccggac cgaacttttt ttccattttt tttcgcgatg
    27721 ggaaaaatta cctaatgtta gcactggtga tggttggcag tgcgctggtg atcgccttag
    27781 ggttaggtaa gctgtttggc tgggatattg gcctgacggc cggtatgtta gcaggctcta
    27841 tgacgtcgac accggttctg gtcggtgctg gcgatacact gcgtcattcc ggcatggaaa
    27901 acaggcagct ctcactggca ctggataatc tgagcctcgg gtatgcctta acctatttaa
    27961 tcggtctggt gagtttgatt gttggtgcgc gttacttgcc gaaattgcag catcaggact
    28021 tacagaccag cgcccagcaa atcgcccgcg aacgtggcct ggacactgat gccaaccgta
    28081 aggtttattt accggtgatc cgcgcctacc gcgtcggccc ggagctggta gcctggaccg
    28141 acggcaaaaa tctgcgtgaa ctgggtattt atcgacaaac tggctgttac attgaacgta
    28201 ttcggcgtaa cgggattctg gcaaatccag acggtgatgc cgtactacaa atgggcgatg
    28261 aaatagcgtt ggtaggctat cctgacgccc acgcccgact cgatcccagt ttccgtaatg
    28321 gcaaagaagt tttcgatcgt gaccttctcg acatgcgtat cgtcactgaa gaagtggtcg
    28381 ttaaaaacca taacgctgtt ggcaaacgcc tggcacaact gaagttgacc gatcacggtt
    28441 gcttccttaa ccgcgtcata cgtagccaga ttgagatgcc gattgatgac aacgtcgtgc
    28501 ttaacaaagg tgacgtttta caagtcagcg gcgatgcccg ccgggtaaaa accatcgccg
    28561 atcgcatcgg ctttatctcg attcacagcc aggtcactga cttgctggca ttctgcgcct
    28621 tctttgtgat tgggctgatg atcgggatga tcaccttcca gttcagcaca ttcagtttcg
    28681 gcatggggaa cgctgccggg ttgttattcg ccggaattat gctgggcttt atgcgtgcta
    28741 accacccgac cttcggttac attccgcaag gtgcattaag catggtgaaa gagttcggct
    28801 tgatggtgtt tatggcaggc gttggtctga gcgccggtag cggtattaat aacggcctgg
    28861 gcgcgattgg cggtcaaatg ttgattgccg gattgattgt cagtctggtg ccagtggtta
    28921 tctgcttctt gttcggtgct tatgtattgc gaatgaaccg cgcactgttg ttcggcgcaa
    28981 tgatgggcgc acgcacctgc gcgccggcaa tggagatcat cagtgataca gctcgcagta
    29041 atatcccggc gctgggctat gcgggcacct acgcaatcgc caacgtcctg ctgacgctgg
    29101 cagggacaat catcgtcatg gtatggccag gattaggata aaactgaagt tgccctgaaa
    29161 atgaaatttt tttgcacaac cgcagaactt ttccgcaggg catcagtctt aattagtgcc
    29221 actgcttttc tttgatgtcc ccattttgtg gagcccatca accccgccat ttcggttcaa
    29281 ggttggtggg ttttttgtta tctaaaactt atctactacc ctgcaaccct ctcaaccatc
    29341 ctcaaaatct cctcgcgcga tagcggctta cggtcggtga caaaatgcag cgtcatcccc
    29401 tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa accattgttc gagcgtttgc
    29461 tgactgcgct gcatccagtt ttgcattacc gtttttaata gcggttttcg gctagccagc
    29521 gcgtagagct ggtacatcag ctccatgtta tccggcgttg caacctgtga gctgtagatc
    29581 atatcggtga tagcctggca tgcgcccgta gcatcactaa catcgctaaa aaatgcctga
    29641 tattgccggg acatgatctc agtaaaactg ctgaacgcct caagcaacaa ctcatcaatt
    29701 cctgaaaaat agtaggtcat cgaccccaac ggtaccccgg caagggtagc aattttgcgg
    29761 tgcgtaacag catgtattcc ataaagtttc accgcctcca gcgtggcctg gataattttt
    29821 tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc ctctctctgc aattgtgtac
    29881 aaatgtacac aaccttgcta atgttgtctt ccctcttctt attttgacgt gatctatgac
    29941 cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg gcgctgttta tgttcttctt
    30001 tttgccgggc ctgttaatgg cgtcctgggc aacccgtacg cctgctatcc gcgatattct
    30061 ttctgtttcg atcgctgaaa tgggcggtgt tctctttggt ctgtcgatcg gttcgatgag
    30121 cggtattctc tgctcggcgt ggttagtgaa acgctttggg acacgtaatg tcatcctggt
    30181 cacgatgtcc tgcgcattga tcgggatgat gatattaagt ctggcactct ggctgacatc
    30241 gcccctgctc tttgccgttg gtctcggcgt ctttggggca agttttggtt ctgcggaagt
    30301 ggcgataaac gttgaaggtg ctgccgttga gcgagaaatg aataagacgg ttttgccgat
    30361 gatgcacggt ttttatagcc tgggcacgct ggcaggcgct ggtgtcggga tggcactgac
    30421 ggcctttggc gttccggcaa cagtgcacat tttattggcg gcgctggtag gcatcgcgcc
    30481 tatttatatc gccattcagg caatccctga cggtacgggc aaaaatgctg ccgatggcac
    30541 ccagcatggt gaaaaaggcg taccttttta tcgggatatc cagttgctgc tgattggtgt
    30601 tgtggtgctg gcgatggcct ttgccgaagg ttctgccaac gactggttac ccttattaat
    30661 ggttgacggt cacggtttta gccctacttc cggctcgctg atttatgccg gttttaccct
    30721 ggggatgact gttggacgct ttaccggcgg ttggttcatc gaccgttaca gtcgcgttgc
    30781 cgtggttcgg gccagtgcac taatgggagc gttgggtatt gggctgatta tttttgtcga
    30841 tagcgcctgg gtcgctgggg tgtctgttgt actttgggga ctgggtgcct cactgggctt
    30901 cccactgacc atttctgccg ccagcgatac cggccccgat gcaccgaccc gcgtcagcgt
    30961 ggtagcaacg accggttatc tggccttcct cgtcggcccg ccgctgctgg gctatctcgg
    31021 cgaacattat ggattacgta gtgcaatgct ggttgtactg gcgctggtta ttctcgcggc
    31081 tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg cagacggcga tggagaatag
    31141 ttgatgagca ttaaattaat tgcggtagat atggatggta ctttcttaag cgatcaaaaa
    31201 acctataacc gtgagcggtt tatggctcag tatcagcaaa tgaaagcaca aggcattcgc
    31261 tttgtggtcg ccagcgggaa tcaatattat cagttgatct ctttctttcc tgaaattgct
    31321 aatgaaatag ccttcgtggc tgaaaacggc ggctgggtag tgagcgaagg caaagatgtt
    31381 tttaatggcg agctgtcgaa ggatgcgttt gctactgtcg tggaacattt gctgacgcgc
    31441 ccggaagtgg aaattattgc ctgcggaaaa aatagtgcct ataccctcaa aaaatatgac
    31501 gatgccatga aaacggtggc ggaaatgtat tatcaccgtc tggaatacgt cgataacttt
    31561 gacaacttag aagatatctt ctttaagttt ggtctgaatc tttccgatga actgattcca
    31621 caagtacaaa aagcattaca tgaggccatc ggcgatatta tggtgccggt ccacaccggc
    31681 aacggcagca tcgatctgat tatccccggc gtacataaag ccaatggcct tcgccaactg
    31741 cagaaattat ggagaataga cgacagcgaa gtggtggtct ttggcgatgg cggtaacgat
    31801 attgagatgc tgcgtcaagc aggctttagt tttgcaatgg aaaatgccgg cagcgcggtc
    31861 gtcgcagcgg caaaataccg ggcaggctcc aataaccgtg aaggcgtact ggatgtgatc
    31921 gataaagtgc ttaaacacga agcgccattt aaccaataaa acggcagcac gcccggcaac
    31981 acaatacctg aatgaaggct gcataattcg cggaaattaa cccgctgcat tatttcaggc
    32041 gaaatgatta tgaaaaattg tctactgttg ggcgcgcttt taatgggctt tactggtgtg
    32101 gcgatggcgc aaagtgtcac cgtcgatgtg ccgagtggat acaaagtggt tgtggttcct
    32161 gattcagtca gcgttccaca agcggtcagc gtcgcgactg tgccgcaaac cgtctatgtg
    32221 gctcctgcgc cagctccggc ttaccgcccg catccttatg ttcgccatct tgccagcgta
    32281 ggtgaaggga tggttatcga acaccagatt gacgaccatc accattaatt cgagaatgcc
    32341 tgatcgcaca aagcaatcag gcattttttt acccttcgtg cgaatttccc atctgtttat
    32401 cttttaaaaa gataaccatc agcaacagcc acaaaattcc gttgacaagg ttgaagagat
    32461 taaacagtcc gttgcccccg ttcagccagg catgtttgct gatttcaata ccgacggtaa
    32521 agatcagcat ttgcagcatc cccatcgccg cagaaaccgt acctttactc atatcgctgg
    32581 caaacagagt taatcgcacc agtcccgcat tcgccagacc aataccgaaa gcataaatac
    32641 ttaacccggc ggtcatccat aaatacgcgt gcgatgagat aaccgttgcc gcagcagcga
    32701 ccaacagacc aatcataatc ggccagccgc ccataataat cagcgaacgt acggtgcgac
    32761 gcgaggtcag acgcgctaac agcaagttac ccgctattaa cgccccgaaa ataggcactt
    32821 gcagcaagcc atattcatag ctgctcaact gctcaccggt aatgatgata atcggcgact
    32881 gagcgatcca cgccagcaac ggcaggctaa cgaatcccag cgccagcgcc cccgcaacaa
    32941 agcgaccgtt cttcagcacc agcttatagt cacgaccgag ttctttcagc gacagtttct
    33001 cgcctatacg cgtggcggtt tcaggcatgg ctcgttgcag accgaaaaag gagatcgctg
    33061 ccaatgcggc aaacaagaca aacatcccct cccagggcag cacatggatc cacgccgcgc
    33121 ccaccagcgg accaagtagc ggagcaatca gcgccacgtt cgccatcagc gcggtgatct
    33181 tgatacaaac cgcctcttcg aaggattcct gaattgcggc gtatcccaca gcgccaatga
    33241 aacagaggct tatgccctgc aagaagcgca acaaagtgaa ttgttcaatg ttttgcgcca
    33301 gtaatattgc cagacaggtg atgataaacc acaccactcc cgccagcatc accggacggc
    33361 gaccaatacg atccgacagc ggccccagga gccattgtaa aaacatcccg cccgccagat
    33421 atgcggtcat cgaagtagga acccaatcaa tgcccgcctg atattgttcc accacggcca
    33481 acataccggg ttgaatcata tcgttgccga tataggttga aaattcgtac agcaccagac
    33541 agagagggaa aagtaacgcc tgacgtccaa gcctggcacc ggaagctaat ttattttgca
    33601 tgcaatttct tcgccaataa taatcgcgca aagtttaata aaagcgcagc taacgagaaa
    33661 gcgaattttg tagctgaaac cacggttaag cacattctta cattattgcg agtatagcta
    33721 cgctttcttt aagttttatt taacctatgc ccgttacaat cacccaccgt aaacaggccg
    33781 cttgagggaa ataagacgat gccgctttac ccagtttaac ctgcacttta ttctcaacga
    33841 cttgcctgta ttggctccct tttaatcact ttgcgtcggg aagttatgct ggaaaatttg
    33901 aatctctctc tattctctct tattaacgcg acgccagact cggctccgtg gatgatctcg
    33961 ttggcgattt ttattgctaa agatttgatt accgtggtgc cgttgctggc cgcgatactt
    34021 tggttgtggg ggcttacagc acaacggcaa ctggtgataa aaatcgctat tgcgctggcg
    34081 gtcagcctgt ttgtgtcctg gacgatgggg catctttttc cgcacgaccg cccctttgtc
    34141 gaaaatatcg gctataactt cctgcatcat gcggcggatg actcattccc gagcgatcac
    34201 ggtacggtga ttttcacctt tgcactggca tttttatgct ggcatcgcct gtggtccggc
    34261 tcacttttaa tggtgctggc cgtcgtcatt gcctggtcgc gcgtttatct tggcgtccac
    34321 tggccgctgg atatgctcgg tggattgctg gcaggtatga ttggctgcct tagcgcccag
    34381 attatctggc aagcgatggg gcataaactc tatcaacgtc tgcaatcgtg gtatcgcttc
    34441 tgttttgcat taccgatccg caaaggctgg gtgcgtgact gatctctgct aaaaagtgta
    34501 gtattgagcg gctcgcttca ataactattc agagggatta tggaaacacg tcgcgaagag
    34561 cgtatcgggc agctgctgca agaattaaaa cgcagcgata agttacatct taaagacgcc
    34621 gccgccctgc ttggggtttc ggagatgacg attcgtcgcg atctgaacaa ccacagtgcg
    34681 cccgtcgttt tgctcggcgg ctatattgtt ctggaaccgc gcagtgccag ccattatctg
    34741 ttaagcgatc aaaaatcccg cctggtggaa gaaaaacgcc gggcggcaaa actggctgcg
    34801 acgctggtag aacccgatca gaccctcttt tttgactgtg gcaccaccac accgtggatt
    34861 attgaagcga ttgataatga aatccctttc accgccgttt gttattcgct aaataccttt
    34921 ctggcactga aagagaaacc tcattgccgc gcgtttcttt gcggtggtga atttcacgcc
    34981 agcaacgcca ttttcaaacc catcgatttt cagcaaacgc tgaataattt ttgcccggat
    35041 atcgcttttt attccgcggc gggggtgcat gtcagtaagg gcgctacctg ttttaatctt
    35101 gaagagttgc cggtaaaaca ctgggccatg tcgatggcgc aaaagcatgt gctggttgtc
    35161 gaccacagta aatttggcaa ggtgcgtccg gcgcgcatgg gtgacctgaa acgctttgat
    35221 attatggtga gcgattgttg cccggaagat gaatatgtga agtacgcgca gacgcagcgt
    35281 attaagttga tgtattaatg acgtataacc ggatgacgtt tcgcgccatc cggttatcag
    35341 aagattaaga gaaccagctg ccgaaccact gatggaattt catcatcacg aaatcccaca
    35401 tccgaccaaa gaatcccccc tcttccacat tttccatcac gatcagcgga cgctgctcaa
    35461 tggatttacc gttaagctgg aaatcaatgg tgccgacaac ctgacctttt ttcagcggtg
    35521 cggtaagctg cggttccgtt aacgtgtagc tcgctttcag gtttttcagc tgcccacgcg
    35581 gtatggtcac tgagcccgct tcgcctgccc cgagattcac ttcgctctta tcaccaaacc
    35641 agacgcgctg agtcacaaag gtggcatcag gtttaattgg cgtcacggtt tcaaagaagc
    35701 ggaaacccca ggtcaataat ttctcagact cattaaaacg gatacggtca gttttcgcac
    35761 ccagtactac ggagatcaaa cgcatatcgc cctgggtagc cgaagcaacc agattatatc
    35821 ccgcgcctgc cgtggttcct gtcttcatgc catcaacatt cagattgctg ctccatagca
    35881 gacggttacg gttaggctgt cgaattttgt tgaaggtgaa ctctttctct ttatgaatgg
    35941 catactcttc cggcacatcg tggatcaatg ctttacccag caatgccata tcgcgcgcgg
    36001 tactgaactg ccccggcgca tccagaccgt gcaccgtctg gaaggtagtg ttggtcagac
    36061 ccagtttttt ggcataacca ttcatcagac caataaatga ctcctggctc ccggcaacgt
    36121 aatcggccag cgcaatacag gcgtcattac cggactggat aattacacct ttgttcaggt
    36181 ctgccaccga aacctgatcg cccggtttaa ggaacattac cgatgaacca cgcagtgccg
    36241 gatttcccgt cgcccaggca tctttaccga ccgtcaccat atcggtgagt ttaatcttat
    36301 cggccttaag cgcctgccca accacatagc tggtcatgat tttagtcagg ctcgcggggt
    36361 ccagtttttc atccgcattg ccttctgcca gcactttacc gctggcgtaa tccattaaaa
    36421 tccatgcacg cgcatccacg ctcggcgctt caacggtttg ttccgccgcg aatgccgttg
    36481 gggcaaaaag gaataaaaat gcagaacccg ctgcaagacc acgaaggaga gaggagtatt
    36541 gcgtcataaa tgccacccga gtatccattc aaaaaaaata cgttatatcg ccgtatgata
    36601 agttcggtga ttaataacgc acacacaaac ttaaagaaac cgtaagttgg taaagttttt
    36661 aaagtttatg caatatcctg ccgcttcgct gcttctggtg tcattttttt gccagctgtt
    36721 gcttaaaagt taactttact tcaccatcgg agtcctgttc agcctgtggc tgcctgtcgg
    36781 aaaaggggtg agtatgatta cgctgtgggg tcggaataat tcaaccaacg taaaaaaagt
    36841 attgctgacg ctcgaagaac tggaactacc ttatgagcaa attctcgcgg gccgtgagtt
    36901 tgggataaat catgatgctg attttctggc gatgaaccct aacgggctgg tgccgttgtt
    36961 gcgtgacgac gaaagtgatc tcattctttg ggaatcaaac gccattgtcc gctatctggc
    37021 ggcacagtac gggcaaaaac gcctgtggat cgactcaccg gcacgtcgtg cggaagcaga
    37081 aaaatggatg gactggacaa accagacgct cagtaatgct catcgcggga tcctgatggg
    37141 attagtcaga acaccaccgg aagagcgcga tcaggccgcc attgatgcca gttgtaaaga
    37201 gtgcgacgcc ctgtttgccc tgctcgatgc ggaactggca aaagtaaaat ggttctctgg
    37261 cgacgagttt ggtgtgggcg atatcgctat cgctccgttc atctacaatt tgtttaacgt
    37321 tggcctgacc tggacaccgc gtccaaatct gcaacgctgg tatcagcaac tcactgaacg
    37381 ccccgcagtg cgcaaagtgg tgatgattcc cgttagctaa ttgcgtgggc taactttaag
    37441 taattcccca ctggactcgt cggtgagaac gtataaataa ccgtcgggtc cagtgcgaac
    37501 atcacgaatt cgctgccctc tgtccgttaa aatacggcca tcttctgtca ctttgtcgcc
    37561 gttgacgctc atcacaatga catctttatc tttcagcgcg ccaataaata atttttgctg
    37621 ccactggggg aatttatcgc tgttatagaa ggccatgccg ctcacggcgg gcgaatcttt
    37681 ccagtaaaaa acaggttgct cggtcccggc gacgatctcc cctttcgctt ccggtatctt
    37741 aaagcctgaa tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg
    37801 cgggatatta atttcatcgc caccgcgcgg gccatgttca ttcagccata gtgcattact
    37861 ccacggattc atcgccattc cttgcggatt acgaatgcca taagaccaga tctcggcgcg
    37921 cgcaccggat tcctttataa aaggattatc atccgggatt tcgccctggt cggtcagacg
    37981 caccagtttg ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc
    38041 cagagcaata aaaagataac ctttaccgtc gaataccagc cgcccgccaa aatggttgcc
    38101 ggtagacagt tttggcatct ggcgaaagac ggtgcggaag tcggtcacct ttgagagatc
    38161 atcacttaag cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga
    38221 ataacttaac cagatgcggc gagactgagc aaaatcaggc gctaaaacca catccagcag
    38281 gccaccctgc ccgtgcgccc aaacttccgg cactccggaa agcggcgcag ataatccttt
    38341 tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattgtc
    38401 gggtaaaaag gccagcgccc agggatggtc gagtttgtcc tgcagtactt cgacatttac
    38461 cgttgcagga gccgcccaga gagcggaaga aagacaaata aggggcacaa ggaaaaagga
    38521 ttgtcgatgc ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat
    38581 aacaaattca tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt
    38641 tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc
    38701 atcagacaat tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc
    38761 ccactcatca aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc
    38821 cactcttcct gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc
    38881 agtttaaaaa agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc
    38941 ttcctcagtt gcacgtatgc ggcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga
    39001 tcgattcgct gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttattgcaat
    39061 taaatagtat ggctattttt tgagcaaaat gctgtccagg atcaattctt cacgccgaat
    39121 taacaaaatt tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat
    39181 ccagaactct gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat
    39241 gagcaaagta actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct
    39301 tgtcgattca gagcgtattc tcaccgaact acgcactgaa ggttatgacg tggtaccgag
    39361 ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca
    39421 agaatcactg gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg
    39481 ttgtctgggg gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac
    39541 cgggcctcat agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa
    39601 acacaaccca ttcctgagcc tggtgccaga acaaggcgtg aaactgacgc cgcgtcatta
    39661 tgcctatctg aaaatttctg aaggctgtaa tcaccgctgc accttctgca ttattccgtc
    39721 tatgcgcggc gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct
    39781 ggttgatgcg ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt
    39841 cgatgttaaa catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag
    39901 cctgtgcgaa cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta
    39961 tccgcatgtg gacgacgtca tcccactgat ggcagaaggc aaaatcctgc cgtatctgga
    40021 cattccgttg cagcacgcca gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt
    40081 agatcgccaa ctcgcgcgca tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg
    40141 ctcaaccttt attgtcggct tccctggcga gacagaagaa gatttccaga tgctactcga
    40201 cttcctgaaa gaagcgcgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg
    40261 tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa
    40321 ccgtttcatg cagctgcagc agcagatttc cgccgagcgc ctgcaagaga aagtgggccg
    40381 tgaaattctg gtgattatcg acgaagtgga cgaagaaggc gcgattggtc gcagcatggc
    40441 agatgcaccg gaaatcgacg gagcggttta tctcaatggt gaaaccaacg ttaagccggg
    40501 tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt
    40561 ttaattaatc ttaaggcaga tctaacaagg atattgttct tcgatctgcc tctgctaccc
    40621 ttcccgatgt cctaccctta cgcacaaaat cccctgacgc ataaatcgag aaaaatgaat
    40681 cgtactcgag gcaactctca ctttttcaca ccaaattaaa gttttgatcg caattacttg
    40741 aaataagcgt aatggttact atagtaaaca ttaccacaag gatggatgta tgggtgtgta
    40801 taaggcaaga cgattcagcc aaagcacgaa aaaacttggt attcacgata aagttctcat
    40861 ggctgccgct gaagaagtga tgcagggcat atgggaggct gatttaggta gcggtgtcat
    40921 caaaaaacgt ttacccctcc aacaaggaaa gagtggtgga gccagaacaa ttatcttttt
    40981 taaatcagca aatcatgtgt ttttttatga tggttggtcc aagagtggtc taagcagtaa
    41041 aggaaccaaa gaaatcgaag atgatgaact ggcggcctac aaaaaaatgg caaatgcatt
    41101 tctggccttt agcaataagc aaatagagga tttgatcgaa actggctttt tgattgaggt
    41161 gaaaaatgag cgataaatta caaatgatgc tggaagatgc aaaggaactg aatgcactgg
    41221 gcctgatgtc tgatagtgaa gttgatgcca ttgccgtcat ggtcaaagct cgtgaagtca
    41281 gcgcccgtat agccaaagtc aaatcaatga cgggtgaaga gatcaaagcc atccgcaccc
    41341 gatacggcat gtcacaatcc gtgctggcac atacaatggg aatgtcaaaa gaaagtgtct
    41401 cgaaatggga acgtaatgaa ataaaaccga gcggcccggc attacgtatt ttgaatacat
    41461 tggcaataaa agggccggaa gtttttgcaa agtaaaatgt ttgcaaatga tttaccacat
    41521 tcattagtac cccttgatta aggggtacta acactattct aattctactg ccagctaatc
    41581 tgcgccctgc cgtttttctt actcttatac agccgctcat ccgccagcat tttcaagcgt
    41641 tcgacggtag tacattcatt tgaatgcgct acaccgatac tggtgcttaa attaatgctt
    41701 cccacctgga aatgaacaat ttcttttaga atcgatagca actcttccag cgttccaccc
    41761 gtataaatca ccgcaaattc gtcaccgccg aagcggtaga ctttaccttt atctacgggg
    41821 atctgtgatt taaggatttt caccactttc atcaacactt catcgcccag caaatgaccg
    41881 aaagtgtcgt tgatgttttt gaatttatcg acgtcaaaca ggcacaaata accgcctttt
    41941 tccggcagcg cttttaaatc ttcgtcaaag gattttctgc ccattgcttt ggtcaggtca
    42001 tccaggaacg ccgagttata cagactgtag agttttttga catcggcagc cgaaaagaaa
    42061 agcccgccat ccagtacgcg ttcaaaatgg ttattaatga tggcttcttt ctccacaata
    42121 ccacgattaa tcactctgcg taccagatag aggcaactga cacccgaaat cactgccacc
    42181 aatgcggcaa agaaaataat caatgtacag acccgcaaat aaacatcttt tttatacgca
    42241 aatacgtaca atccggtcgg caaataggcg aagtgctcca tcgcttccga aacggtgaag
    42301 atggcgtttt tagcattaaa ttcatcaccg gtcaccggtt taccgttctt aaacagagag
    42361 aacatcaggt tctcgctatc catcgccagc gattttgaca gtaaagaaaa acgctccaga
    42421 ccaagcaaaa atatcagact acgattatga gttaccggat agaggtaata gacgaaggtt
    42481 ttaccgtcaa aagagataaa ccctgtattt ttgacatgtc gatacttcac aatctgcata
    42541 atatctgcac aataattttt ctcgaactcc tttacctgaa tacattcgtc actggaaatc
    42601 agataaggac cgacatcgta caaataagag ggtgaaaccg agtcggcgta agctgtgcgc
    42661 aaaccaacca acagctgttc ctccgtgggg tttagcgtgt tgatgagatc aattgccacg
    42721 ctacgccgat attcagcaat atttgccacc gcaaaagcag tatcaataac ggactgtttc
    42781 aggccataca cccgttcctt taccatttga gtgtagatcc cgcaggcaac tgctgaaatc
    42841 atgataaaaa tcagcagact gactgtaagt acgaacttat tgattctgga catacgtaaa
    42901 ctactctttt actaattttc cacttttatc ccaggcggag aatggcatgg gtttctggta
    42961 cagatacccc tgagcctgaa cacatcctaa tttgattaat tcgcgcgcaa cgtcttcgct
    43021 ctctacgcct tccgcaatca ccgggatccg gttatagcgt gccagatcga aaataatgcg
    43081 cacaattttt cccgcagtac cgaaaatatc gccggtaaat tgtgagtcca cttttatgct
    43141 atctggatta atttcataaa aacgtttcag gtttgacaaa ccggtgccaa agtcatcaat
    43201 cgatatctgt accccttgtt tacgcagttg ttcaatattg agataaaaat gttcattaat
    43261 tcgctcgatc acggtttcag tgatctcaac acagaggctg ttctccttca ggccattttc
    43321 tttcatgatg gtggtaaaac gctcatgaaa gtgtggttcg tttaactgcc agggcgaaac
    43381 attaatatgc agtaagaaat catccttcag cgacgcacgc tgactaaaat gtctgaattc
    43441 acccattgcc gttttaataa tctgataacc cagctcattg ataaacccga tatcttcagc
    43501 aatggtaatg aactttagcg gtgaaatgat cccccgctcc gcagacaccc aacggcacaa
    43561 cgcctccgcg cccagaatgg cgcgggtgtt aatatcaacg atcggttgat agacgaggaa
    43621 tagttcgcca atttccattg catggcgaat attacgcgca atccagatat tctcttctat
    43681 ttcgttgagt ttcgcgtcat cacagaaaat aaactctgag aacatatggt cacggatact
    43741 ggaaatcgcc agtttcagtt tttctgcgta actccaggcg ttttctccct gcaaaggttc
    43801 acaaatggca gacttatgga tgcgcagatt gcggtagcca aagctgctgt caaaatcgtt
    43861 tatcatcgct attttacggg taaatgtctg tacattttcc cggcacagaa gtaaatagag
    43921 atcggcattg aatgtcacga tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca
    43981 caatttcctg acgattttat tgcgttcatc aacaccaaac accgcttcca cggcttccag
    44041 accacacagt ttgatgatcc ccacagccag atactgatta ttgttggact caataatcgc
    44101 catattttta ataaaaccgc tttcctgatc gtaagagatc agtgagagca atttatttga
    44161 ccgttcaatt tcggcatctt tcatttccgc gatcgaattt ttgatgctga taatctcttt
    44221 cgcatcgcct tcgcgaattt tcagagcctc gatttgatcc gttgagtcag gcaaaaacgc
    44281 cagcgtatta accagctctg tcatttcaga aaattgccga cggaacacac gagtggtcat
    44341 taaataaacc accagcaata cgataataat gaaagcaaac ggcagataaa aaagtttatc
    44401 tgatgcgttc aataaattac caccaggata ataaaaatag atttcgaata atgcgttgtt
    44461 tggcaccgct gaagtttttt cgatatattc agtatcgaca gaatcaataa tattataatt
    44521 accttcgcta ttataaataa ttagtttttc acgagcgagt tgattcttgg caatgatctc
    44581 atggccttgt tttaaaaata gaacttcatc attagcgctg atttgatcaa gatactcgtt
    44641 cagatatcgc cagtttaaat cgacatgaat tttatccacc gctaacaaat catgatcttt
    44701 gccgttgatg tgtagtgtaa aagagataca ttttttaccc ggacgatcaa agcaattgta
    44761 tgccggattc cagctgttgt tagagtcatc tgaagccacc gaccacggtc gtttcgatgg
    44821 gttaaaatcg gcaaaaatta cccgccctga ctcgtcgaca agcggtccat ttacttgttc
    44881 ctgatgataa acaacgattt tatcattcgc cctccgcgaa aaaagataat atttattatc
    44941 atcaagtacc agtcccaccg agtctaaaaa tggcatcatt tccattttaa gaatcacggc
    45001 gcgttgaatc tcctctgtat tttttaataa atacagtgag tcctttaatg tcagggccga
    45061 acgttcaata tctttaaaga aatagccaac gtttttctga ataagttcaa agcggctatc
    45121 ggcacgtttt accgcgcgct ccgataccag ttgatagtta atgatgaaaa aaagaccaat
    45181 aaatgacagt gctgccaata aaaataaaat tatcagcctt atctttatct tttcgtataa
    45241 actcagcatg ttaatacatt taacaaaata actatctgaa cgctgtgccg ctgcaaaaat
    45301 tgtcccttgc tgcggctttt taaccaaatt acccgattct acaaaatcag cagaaagtta
    45361 caacacttgc ttagggcaat aatttcctgt gaggtgatta ccctttcaag caatattcaa
    45421 acgtaactat cctttaattt tcggatccag cgcatcgcgt aaaccatcgc ccaacaaatt
    45481 gaacgccagt acggtcagaa aaatagccag ggccggaaaa acagcgacat gcggcgcgat
    45541 aaccatatcc gctcgtgcct cattgagcat tgctccccac tctggtgtcg gcggctgcgc
    45601 accgaggccg agaaatgaca ggctggcggc agagataatc gaggtgccga tgcgcatggt
    45661 gaaaaacacc acgatagaag agacggtccc aggcaagata tgacgcaata aaatggtcat
    45721 atcgctggca ccaatactgc gtgctgactc aataaaggtt tgctgtttca acaccagcgt
    45781 gttgccgcgc accaggcggg caaacgcggg gatggaaaaa atggcgacag cgataatcac
    45841 gttagcaatg ccgcttccca acaccgcaac aacagcgatc gccagtaaaa tacccgggaa
    45901 ggcaaacagc acatcgcaaa tgcgcatgat tagccgatcc caccagcctt cataatatcc
    45961 agcaagcaag cccagcaacg tcccgatcgc cgcaccgata aacacggcaa acacacccgc
    46021 cgccagtgag atttgcgcac caaccaggac acggctgaaa atgtcgcgcc ccagtgaatc
    46081 gacgccaaac cagtgctgca aagaaggtcc gttattcaga ttgtcataat cgaaataatt
    46141 ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc actacaatca ataaaataac
    46201 gaataatgcg gcggtcatcg ccatatgctg acggcgaaat cgtcgccaga attcatgcca
    46261 cggtgtacgt acctggtcag gtttgaccag tggcatggcg tttaacgccg cctgacgtcg
    46321 ccagttaaat agtcgcatcc ttacttgtac ctgatagccg ggttaatggc ggcgtaaagc
    46381 acatccacca ctaagttgat aagaataaat tccagcgaga aaagcagaat ttccgcctga
    46441 atcaccgggt aatcgcgcat ttctacggag tcaacgagta agcgtccaag tccgggccag
    46501 ttgaaaacct tctccaccac gatggaacca ccgagcaaga agccaaactg taagcccatc
    46561 atggtcacta ccgggatcat cgcgttacgt agcccgtgtt tgaggacaac ccaggtttcg
    46621 ctcacccctt tcgccctcgc ggtacgcata taatcttcgc ttaaaacatc gacaaacgac
    46681 gcgcgggtaa agcgcgccat cacggctgcc actgccgcgc cgagcgtcag ggagggtaaa
    46741 atgtagtgct gccaactgtc tgctcccacg gtaggcagcc agcccagttc aacggagaat
    46801 acctgaatta aaagcatccc cagcgcaaat gccggaaacg agatccccga caccgcaatg
    46861 gtcatactca atcgatccgg ccaacgatta cgccagacgg cggcgataat tcccgccgcc
    46921 ataccaaata tcaccgccca gaccatactg gttatggtca gccacagcgt tggcataaag
    46981 cggctggcaa tctcatcggc aaccggacga cgcgacacca tcgacaggcc aaaatccccc
    47041 tgcacagcat tgctgatata gtgccagaac tggtgataca gcggctgatc caaccccagc
    47101 tgctgacgca ccagttctat aacctgcgca tcagcttcgg gcccggcaat caatcgcgcc
    47161 ggatcgccgg gcagcatatg gacaaataaa aacaccagca ccgagacgat aaacagcgtt
    47221 ggaatcaacc ccagtaagcg tttgataacg taattaagca ttccactccc tgcgttgctt
    47281 attgcaaatc cgcgtcttca aagctgaaac cggtgtctgg catgatccaa aaaccggtca
    47341 ggtttttact gtgtgccgac accagttttt ctaccaccag cgggatccac ggcgactctt
    47401 gccagatgat atcctgcgcc gccttatata aacgggtctt ttccgccgga tcattagttt
    47461 tcagtgcctg agctaggaag tcatccacct gtttattgct gtaaaacgcg gtattaaaca
    47521 gcgtcggtgg ccagttttgc gatgcaaaca gcggcgatag tgcccagtca gcttcgccgg
    47581 ttgaagccga ccagccagtg tagaacatcc tcactccgct ctctttttgc cctttacctt
    47641 caacttctgc cgcccgatgt ccggcatcca tcgcagtcac ctgggcttta atcccgacct
    47701 gcgctaactg ctgctgggta aattgcagca ctttctgcgc ggtgctgtgg ttatgtgacg
    47761 accacagcgt ggtactgaaa ccgttgggat atcccgcctc tttcagtaat tcgcgcgctt
    47821 tcactggatc gtaaggccac ggtttataac tttgcgcgta ggcgatactc ggcggtacca
    47881 caccagtagc tggcgttgca tagcccgcga aggctacttt caccagcgcc gggcggttaa
    47941 tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa cggcttttgc gtcacgttca
    48001 tactgatata acgctgcata attgacggac tggccatcaa ctcgatattt ttgtttttct
    48061 ccagcagtgc ggcctgctcg tagggaatgg ggaaagcaaa ctgcgcttca ccggtttgca
    48121 gcattgccgc gcgggtattg ttatccgcca ccggacgcca ggttatgctg tccagtttgg
    48181 gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt cacaaaatcg gtctgattcc
    48241 aggtatccag ttcatacggc ccggttccca ccggatgaaa accaatctcc ttgccatatt
    48301 tttccagcgc tgccggcgaa atcatcgcgg tcgccggatg ggcaagaata ttaataaacg
    48361 ctgagaacgg ctgtttgagg gtaatcttta ccgttgttgg atcgatcgct tccgttttgg
    48421 caatattctt atacaggttg tagcgtttaa gatgattcgc cggatcgctg gcccggtcca
    48481 gattcgcttt taccgccgct gcgttgaagt cggtgccatc ctggaattta attccttccc
    48541 gcaatttcac ggtgtaagta aggccgtcat cggaaacggt ataactctcc gccagcacgt
    48601 ttttcagttt catctcttta tcgagaccga acagcccctg gtaaaacgat ttcgctacgg
    48661 cctgagataa cgtgtcattt gcgtcatacg gatcgagcgt ggtgaagttc gatcctaccg
    48721 ccaccaccac atctttggca gcgaatgcac aagatgccat caacgctgtc gcaatgccca
    48781 gcgccactaa cccactacgg tgtacagctc ttgccatttt attctccgcc tgaatgttat
    48841 ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt aatgccccgg cccgacgcat
    48901 tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa tattgctggg aagatcgtcc
    48961 gacagcagta cacgctgcgg tcgttgtcgg gacggttcag cgaccggaac tgccgccagt
    49021 aatttacgcg tataaagatg ctgcgggttt tcgaagaccg cgcgccgtgg accaatttca
    49081 acaatttgtc cgagatacat caccgccaca cgatgactaa tccgctctac cacggccata
    49141 tcgtgggaga taaacagata cgcaatgccg aaatcacgct ggagatcgag caacaagttg
    49201 ataatctgcc cgcgaataga aacatccagc gccgaaacgg cttcgtcggc aatgatcact
    49261 tttggattca atgccaacgc gcgagcaatg cagatgcgct ggcgctgacc gccggaaaac
    49321 tcatgcgggt aacgccaggc atgttcaggt aacaggccca cgcgctccag caaccacgca
    49381 acgcgtgcag ccgcttcttt acctgtcaat aaaccgtgta cgcgcagcgg ttcgataatc
    49441 gaatcaccga tggtctgacg tgggtccagc gaagcgtaag ggtcctgaaa aataaactga
    49501 atatcccggc gcaatgcctg aagtttgccg ggtgacaagg tatcgattcg ctgaccgtta
    49561 aagataattt cgccgccctg cgattcgacc aggcgcagca acgcccgccc ggtagtggat
    49621 ttaccgctgc cagactcgcc caccagcgat agcgtttcgc caggccagag atcaaaactg
    49681 actttctcaa cggcatgcac ttcccgcgtt acgcgattca acaaaccgct gcgcaaaggg
    49741 aaacgggtga ccagattacg cacccgtaaa acaggttcgc catccaccac cgttttctgc
    49801 tcgatgggtg gttcctgttt cgctggatgt tcaagcgata tcaatgggaa acgtcgggga
    49861 taatctaacc ctttcatcgc accaagttgc ggaacagcag ctaacagcgc acgggtgtaa
    49921 ggatgttgcg gtgcatgaaa aatctgttcg acgctacccg tttccaccgc ctcgccctga
    49981 tacatcacca gtacccgatc ggcaatctct gccaccacgc ccatatcgtg agtgataaag
    50041 ataacgccca tcgacatctc tttttgcaat actttgatta attgcaggat ctgcgcctga
    50101 atagtgacat ccagcgcggt ggttggctca tcggcaatca gcaccgccgg gtggcatgac
    50161 agcgccatcg caatcatcac tcgctggcgc atcccgccag agagttgatg cggataacgt
    50221 gaaaggatgg tttgtgcctc cggaatgcgt acctgatcca gcatccgctt cgcctcgacc
    50281 attgcttctt cacgactggc gttttgatgc agacgaatag attcagcaat ctgttcaccc
    50341 acagtaaata ccgggttcag cgatgtcatc ggctcctgaa atatcatcgc catatccgca
    50401 ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa gttcaatcac ttcacgactg
    50461 cgccgccgca acagcatttg atcgcactgc actaaaccgc ccgcctgttc caacaagcgc
    50521 atcaatgcca gcgcagtcac tgacttacca gagccggatt cgccaacaat tgccagcgtc
    50581 tcaccgcgtt gcaggctaaa agagagattg cggaccacag ctattttctg ctggtcctgc
    50641 ataaaggcaa tattcagatt ttcaaccgcc agcacatcac cggcatcaag ttcatcactg
    50701 tgtggcaacg gtgtcccctt tttcacggta gataccggtg gttggcgtat cgcctgcgta
    50761 gccccaggcg cgatacattc cttcggtgtt aaacggtagc gcgacattcc cttcatggtc
    50821 gatagcgatt aagccaccgc taccgccaag cgcagggagt ttttccatta ctacccgctc
    50881 gcaggcttcc gcgagactta atccgccgta atccattaac gcggcgatgt catatgccgc
    50941 cagcgcgcgg atgaagactt cgcccgtgcc ggtacaagaa accgccacac tggcgttatt
    51001 ggcgtagcat ccggcaccca ctaagggact atcgccaact cgtccgggta atttattggt
    51061 cattccgccc gtggacgtgg ctgccgccag attgccgtct aaatccaacg ccacggcccc
    51121 cacggtgccc attttctgtt tttcatccag ttgcgcaccg ctatggtcga ggactgttgc
    51181 cccttcctca cgcgctgcca ttagttgttc ataacgcaaa ggcgtggaga aaatctccgg
    51241 tgagacgcgc tccatgccat gagcaaacgc aaaattttct gccccttcgc caatcatcat
    51301 cacatgcggg ctttgctcca tcaccagccg ggcggcaaga accggattac gcagatgact
    51361 aacgcccgcc accgcaccgg ctttcagggt gttaccatcc atcacacagg cgtccagttc
    51421 atgggtttca tcacgcgtaa agacagcgcc aattccggcg ttaaacagtg gacactcttc
    51481 cagcagacgc accgcttccg tcaccacatc cagcgcactt tcgcccgctt ccagcatttt
    51541 ctgcccggtt tcaacaatgg cagacagcgc ctcgatgtag cgtaattcct gttgcagact
    51601 catctgcgcg cggctaattg cacctgcgcc gccatgaatt gcaatgactg ctttgcccat
    51661 agtattcatc cattaagggt ggctcgccac ttttgctata aatatcagaa tttttatgaa
    51721 ttactcattc aatttttgaa tatagaaaga tggatatggc atgtaaaggc aggagccagc
    51781 gctacgcccg aggtggcata cagtctgtgg cgtttttctg acataatagg caaattcgat
    51841 tttgcctccg caggagtgtt ttcatggaat ttaccaccgg attgatgtcg ctcgacaccg
    51901 cgcttaatga gatgctttct cgcgtcaccc cactgaccgc ccaggaaacg ctgccactgg
    51961 tacagtgttt tggtcgtatt ctggcgagcg atgtcgtttc gccgcttgat gtcccggggt
    52021 ttgataactc cgctatggac ggctacgcgg tgcgtttagc cgatattgcc tccgggcaac
    52081 cgctgcccgt tgccggtaaa tcctttgccg gtcagccgta ccatggtgaa tggcctgcgg
    52141 gtacctgcat tcgtattatg accggtgcgc cggtgccaga aggctgcgaa gcggtggtca
    52201 tgcaggaaca gactgaacaa acggacaatg gcgtgcgttt tactgctgaa gtgcgtagcg
    52261 ggcaaaatat tcgccgtcgc ggtgaagata tctctgcggg tgcggttgtt ttcccggcgg
    52321 gaacacgcct gactaccgca gagctgccag taattgcttc actggggatt gccgaagttc
    52381 cggtgattcg taaagtgcgt gtagcgctgt tttctaccgg tgatgaactc cagttgccag
    52441 gccagccgct gggcgacggc caaatctacg ataccaaccg tctcgccgta cacctgatgt
    52501 tagaacagct gggatgcgag gtaatcaact tagggattat ccgcgacgat ccccatgccc
    52561 tgcgcgccgc ttttattgaa gccgacagcc aggcggatgt ggtgatcagt tccggcggtg
    52621 tttcagtggg tgaagcggat tacaccaaaa ccattcttga agagctgggg gagatcgcct
    52681 tctggaagct ggcgattaaa ccaggtaaac cgttcgcgtt cggaaaactt tctaatagct
    52741 ggttctgcgg cctgccgggc aacccggttt cagcgacgct gaccttctat caactggtac
    52801 agcctttgct ggcaaaacta agcggcaata ccgccagcgg cctgcccgcg cgccagcgtg
    52861 tgcgcacggc atcgcctctg aagaaatcgc caggacgcct tgatttccag cgcggcgtgc
    52921 tgcaacgcaa cgccgatggc gaactggaag tgacgaccac cggacatcag ggttcacata
    52981 tatttagctc ctttagcctc ggtaactgct ttatcgtgct ggagcgcgat cgcggcaatg
    53041 ttgaagcggg cgaatgggtg gaagtagaac cgtttaacgc gttgttcgga ggcctgtaat
    53101 ggcggaactc agcgatcagg agatgctgcg ctacaaccgg caaatcattc tgcgcggttt
    53161 tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg ctgatcgtcg gcctcggtgg
    53221 actcggctgt gcagcttcgc agtatctggc aagcgccggt gtcggcaacc tgacgctgct
    53281 cgacttcgac acggtttcgc tctcgaatct gcaacgccag acgctgcaca gcgatgccac
    53341 ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg acgcggatca acccacatat
    53401 cgcgattacg ccagtcaatg cgctgcttga tgacgctgaa cttgctgcga tgattgcaaa
    53461 acacgatctg gtgctcgact gtacggataa cgttgcggta cgtaatcaac tgaacgcagg
    53521 ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca attcgtatgg aaggtcaaat
    53581 cacggtcttc acttatcagg acggcgagcc ttgctatcgc tgcctcagcc gtttgtttgg
    53641 cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca ccgttaatcg gcgtaattgg
    53701 ttcgttgcag gcaatggaag caatcaaact gctggcaggt tatggcaaac cagccagcgg
    53761 gaaaatcgtg atgtacgatg cgatgacctg tcagtttcgc gaaatgaaac tgatgcgcaa
    53821 tccggggtgt gaggtgtgcg ggcagtaact gccacctcca ggccagataa gacgcggcac
    53881 tccgccacgt catcagcaat ttatgaggat gcagaacgtc cggttaaatc gacgttctgc
    53941 caaacgctcc ctgccagtcc tgctcaaact tcgccacagc ggcatcaacc gccggatagc
    54001 taatcatctg ttgtgccaca tccagcggca gagtaattga ttcacaccct gccagtaagc
    54061 agtccagcgc ctgacgcggg gttttaaaac tcgcggctaa cactttcgcc tgcggcgcat
    54121 gcattttcaa taactggtgt aagtcggtca cagtctgaat accgctaccg ccctgagcat
    54181 caatacgatt aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac agtagccctt
    54241 gtgctgcgcc atataccgcg gttcccagcg tcggaatccc ttccgctttt aacatcttaa
    54301 tcgctgccag cccctcagcg gtcaccggaa ctttcaccac gatatccgca ataatagaac
    54361 gcaatttacg tgcgtcattc accatccctt cagcagtggt agccattacc tgggcaaaca
    54421 gacgcccctg accgtccatc gcctcgtgaa gttgcggaag cacaacctcc agcggttttt
    54481 tacccgctgc gataatgctt gggttagtgg tcacacccgc cagcggaaaa atacgtgaca
    54541 gcgccttcac cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa
    54601 tgtacttttt gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacgcacat
    54661 caatctaact ttcattcgaa agtattttaa tgtttgaacg aaacagagag gcaactatga
    54721 ttttcaatat tcaacgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc
    54781 ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc gcccgcacgc
    54841 aggatctgct gtatgaccca cgtctgtgcc tggaaggctg tgagctgtgc gctaagaccg
    54901 cgccggacgt gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa aagttaaccc
    54961 cggagcatct gacggcgtta accgactgct gtccgacaca ggcgttaacc gtgtgtggtg
    55021 aagtgaaaag cgttgaggag atcatggcga ccgttttgcg ggataaaccg ttttacgatc
    55081 gcagcggcgg cggcttaacg ctttccggcg gtgaaccatt catgcagccg gaaatggcga
    55141 cggcgctgct gcaagccagc cacgaggccg gcattcatac tgcggtagaa acttgtctgc
    55201 atgtgccgtg gaaatatatc gccccttctc tgccctatat cgatctgttt cttgccgatt
    55261 taaaacacgt tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagagtgc
    55321 tggataacct gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc gtgccgctga
    55381 ttcagggctt taatgccgac gaaacctctg taaaagccat taccgatttt gccgccgacg
    55441 agctgcacgt tagcgaaatt catttcctgc cctaccacac gctgggcatc aacaaatatc
    55501 acttacttaa tctgccctat gacgccccgg aacaaccgct tgatgcgcca gaactgctcg
    55561 actttgccca gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac
    55621 catgaccaca ctgaaactgg acacgctcag cgatcgcatt aaagcgcaca aaaatgcgct
    55681 ggtgcatatt gtgaaaccgc cggtctgtac cgagcgcgcg cagcactata ctgagatgta
    55741 tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg ctggcgctgg cgcatcacct
    55801 ggcgaatcgc accatctgga tcaagcacga tgagttgatc attggcaacc aggcaagcga
    55861 agttcgcgcc gcgccgatct tcccggaata taccgtctcc tggatcgaaa aagagattga
    55921 tgatctggca gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca aacgtgttct
    55981 gcatgaagtg tgcccttggt ggcgcggtca gacggtacag gatcgctgct acggcatgtt
    56041 taccgatgag caaaaaggtc tgctggcgac cggaatcatt aaagcggaag gcaatatgac
    56101 ctccggcgat gcgcacctgg cggtgaattt cccgctactg ctggaaaaag gtcttgatgg
    56161 tctgcgcgag aaagtagcgg aacgtcgctc gcgcatcaac ctgacggtgc tggaagattt
    56221 acatggcgag cagttcctga aagcgattga tatcgtgctg gtggcagtca gtgaacacat
    56281 tgaacgtttc gctgccctgg cgcgtgaaat ggccgcgacc gaaacccgcg aaagccgtcg
    56341 cgatgaactg ctggcgatgg cagaaaactg cgatcttatc gcccaccagc cgccgcagac
    56401 tttctggcag gcgctgcaac tgtgttactt catccagttg attttgcaga tcgaatctaa
    56461 cggtcactca gtatcttttg gtcgtatgga ccagtatctg tacccgtatt atcgtcgcga
    56521 cgttgaactc aaccagacgc tggatcgtga acacgccatc gagatgctgc atagctgctg
    56581 gttgaaactg ctggaagtga acaaaatccg ctccggctcg cactcgaaag cctctgcggg
    56641 aagtccgctg tatcagaacg tcaccattgg cggacaaaat ctggttgatg gtcaaccaat
    56701 ggacgcggtg aatccactct cttacgcgat cctcgaatcc tgcggtcgcc tgcgttccac
    56761 tcagcctaac ctcagcgtgc gttaccatgc aggaatgagc aacgatttcc tcgacgcctg
    56821 cgtacaggtg atccgttgcg gcttcgggat gccggcgttc aacaacgacg aaatcgtgat
    56881 cccggaattt attaaactcg gtattgaacc gcaggacgct tacgactatg cagcgattgg
    56941 ttgtatcgaa accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat
    57001 caacttcgcc cgtgtgatgc tggcggcgct ggaaggcggt cgtgatgcca ccagcggcaa
    57061 agtgttcctg ccacaagaaa aagcgttgtc ggcagggaac tttaacaatt tcgatgaagt
    57121 aatggacgcg tgggacacgc aaatccgtta ctacacccgc aaatcaatcg aaatcgaata
    57181 tgtcgttgac accatgctgg aggagaatgt gcacgatatt ctctgctcgg cgctggtgga
    57241 tgactgtatt gagcgagcaa aaagtatcaa gcaaggcggc gcgaaatatg actgggtttc
    57301 tggcctgcag gtcggcattg ccaacctcgg caacagcctg gcggcagtga ggaaactggt
    57361 gtttgaacag ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga
    57421 tggcctgact catgagcaac tgcgtcagcg gctgattaac ggtgcgccga agtacggcaa
    57481 cgacgatgat accgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact
    57541 gaaacagtac cataacccac gctacggtcg tggtccggtt ggcggcaact attacgcggg
    57601 tacgtcgtct atctccgcta acgtaccgtt tggcgcgcag actatggcaa caccggacgg
    57661 gcgtaaagcc cacaccccgc tggcagaagg cgcaagcccg gcttccggta ctgatcatct
    57721 tggccctact gcggtcattg gctcggtggg taaactgcct acggcagcga ttctcggcgg
    57781 cgtgttgctc aaccagaaac tgaatccagc aacactggag aacgaatctg acaagcagaa
    57841 actgatgatc ctgctgcgca ccttcttcga ggtgcataaa ggctggcata ttcagtacaa
    57901 catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat cccgatcagt atcgcgatct
    57961 ggtagtgcgc gtcgcgggct attccgcctt cttcaccgcg ctctctccag acgcacagga
    58021 cgatatcatc gcccg
//
LOCUS       DAPOZO010000020        57937 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPOZO010000020 DAPOZO010000000
VERSION     DAPOZO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.020"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            303..668
                     /locus_tag="ROI21_001316"
     CDS             303..668
                     /locus_tag="ROI21_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDX5110263.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            910..1266
                     /locus_tag="ROI21_001317"
     CDS             910..1266
                     /locus_tag="ROI21_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDX5110264.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(1317..2213)
                     /gene="yhaJ"
                     /locus_tag="ROI21_001318"
     CDS             complement(1317..2213)
                     /gene="yhaJ"
                     /locus_tag="ROI21_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDX5110265.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            2318..3019
                     /locus_tag="ROI21_001319"
     CDS             2318..3019
                     /locus_tag="ROI21_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDX5110266.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQTSTGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHYEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            3042..3206
                     /locus_tag="ROI21_001320"
     CDS             3042..3206
                     /locus_tag="ROI21_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110267.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTANNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(3339..4649)
                     /locus_tag="ROI21_001321"
     CDS             complement(3339..4649)
                     /locus_tag="ROI21_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDX5110268.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(4677..6008)
                     /locus_tag="ROI21_001322"
     CDS             complement(4677..6008)
                     /locus_tag="ROI21_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDX5110269.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(6283..7647)
                     /gene="tdcG"
                     /locus_tag="ROI21_001323"
     CDS             complement(6283..7647)
                     /gene="tdcG"
                     /locus_tag="ROI21_001323"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDX5110270.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(7719..8108)
                     /locus_tag="ROI21_001324"
     CDS             complement(7719..8108)
                     /locus_tag="ROI21_001324"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDX5110271.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(8122..10416)
                     /gene="tdcE"
                     /locus_tag="ROI21_001325"
     CDS             complement(8122..10416)
                     /gene="tdcE"
                     /locus_tag="ROI21_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDX5110272.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(10450..11658)
                     /gene="tdcD"
                     /locus_tag="ROI21_001326"
     CDS             complement(10450..11658)
                     /gene="tdcD"
                     /locus_tag="ROI21_001326"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDX5110273.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(11684..13015)
                     /gene="tdcC"
                     /locus_tag="ROI21_001327"
     CDS             complement(11684..13015)
                     /gene="tdcC"
                     /locus_tag="ROI21_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDX5110274.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(13037..14026)
                     /gene="tdcB"
                     /locus_tag="ROI21_001328"
     CDS             complement(13037..14026)
                     /gene="tdcB"
                     /locus_tag="ROI21_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDX5110275.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(14125..15063)
                     /gene="tdcA"
                     /locus_tag="ROI21_001329"
     CDS             complement(14125..15063)
                     /gene="tdcA"
                     /locus_tag="ROI21_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDX5110276.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            15252..15596
                     /gene="tdcR"
                     /locus_tag="ROI21_001330"
     CDS             15252..15596
                     /gene="tdcR"
                     /locus_tag="ROI21_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDX5110277.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQANFELNLMS
                     KFSNFIINKSFSAINNAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            15852..16392
                     /locus_tag="ROI21_001331"
                     /pseudo
     CDS             15852..16392
                     /locus_tag="ROI21_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16414..17601
                     /locus_tag="ROI21_001332"
     CDS             16414..17601
                     /locus_tag="ROI21_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HDX5110278.1"
                     /translation="MSPVSSIGNDISSDLARRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIEMVTLLNALQPGGKCIITGDFQKELAYLQNVILYNDSSLRMDFFGYNARIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGGIVDLSSLDLRN
                     VDLELYDFTDKHMANTILNPFKLDDTDFTNANMFQVNFVSSKQNATISWDYLLKITPV
                     ITSINDMYSEEKIKLVESCLNALGDITEEQLKIMRLAIIESIPRATLTDELENELTKE
                     IYKSSSKINNYLSRIKLPEMKAFSLEKIDYYIDIIIKDYESVKENAYLIVPKINYNTD
                     LNIEDSSSEEFLPDNTIEKDENSPDNCFEVVKYNTYEAYNSEKQYYTREDYTYDYDLL
                     NAI"
     gene            complement(17992..18368)
                     /gene="rnpB"
                     /locus_tag="ROI21_001333"
     ncRNA           complement(17992..18368)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ROI21_001333"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(18401..19546)
                     /gene="garK"
                     /locus_tag="ROI21_001334"
     CDS             complement(18401..19546)
                     /gene="garK"
                     /locus_tag="ROI21_001334"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDX5110279.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYNKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNIYRASRNIAATLAIGMRNAG"
     gene            complement(19643..20533)
                     /gene="garR"
                     /locus_tag="ROI21_001335"
     CDS             complement(19643..20533)
                     /gene="garR"
                     /locus_tag="ROI21_001335"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDX5110280.1"
                     /translation="MTMKVGFFGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQAVRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(20563..21333)
                     /gene="garL"
                     /locus_tag="ROI21_001336"
     CDS             complement(20563..21333)
                     /gene="garL"
                     /locus_tag="ROI21_001336"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDX5110281.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(21349..22683)
                     /gene="garP"
                     /locus_tag="ROI21_001337"
     CDS             complement(21349..22683)
                     /gene="garP"
                     /locus_tag="ROI21_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDX5110282.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            23058..24629
                     /gene="garD"
                     /locus_tag="ROI21_001338"
     CDS             23058..24629
                     /gene="garD"
                     /locus_tag="ROI21_001338"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDX5110283.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            24778..25113
                     /gene="prlF"
                     /locus_tag="ROI21_001339"
     CDS             24778..25113
                     /gene="prlF"
                     /locus_tag="ROI21_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDX5110284.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            25113..25577
                     /gene="yhaV"
                     /locus_tag="ROI21_001340"
     CDS             25113..25577
                     /gene="yhaV"
                     /locus_tag="ROI21_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDX5110285.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH"
     gene            complement(25632..26441)
                     /gene="agaR"
                     /locus_tag="ROI21_001341"
     CDS             complement(25632..26441)
                     /gene="agaR"
                     /locus_tag="ROI21_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDX5110286.1"
                     /translation="MSNTDASGEKRMTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            26690..27970
                     /gene="kbaZ"
                     /locus_tag="ROI21_001342"
     CDS             26690..27970
                     /gene="kbaZ"
                     /locus_tag="ROI21_001342"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDX5110287.1"
                     /translation="MKHLTEMVRQHKEGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQSLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWS
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGKLQPTPRELIINHIQDILAQYHTACEGQ"
     gene            27993..28466
                     /gene="agaV"
                     /locus_tag="ROI21_001343"
     CDS             27993..28466
                     /gene="agaV"
                     /locus_tag="ROI21_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDX5110288.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            28477..29256
                     /locus_tag="ROI21_001344"
     CDS             28477..29256
                     /locus_tag="ROI21_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDX5110289.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            29246..30124
                     /locus_tag="ROI21_001345"
     CDS             29246..30124
                     /locus_tag="ROI21_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDX5110290.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AITVVLGIVGKFCHFL"
     gene            30142..30576
                     /locus_tag="ROI21_001346"
     CDS             30142..30576
                     /locus_tag="ROI21_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDX5110291.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            30573..31706
                     /gene="nagA"
                     /locus_tag="ROI21_001347"
     CDS             30573..31706
                     /gene="nagA"
                     /locus_tag="ROI21_001347"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDX5110292.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTDVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            32057..33211
                     /locus_tag="ROI21_001348"
     CDS             32057..33211
                     /locus_tag="ROI21_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDX5110293.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRVILTGAGTSAFIGDIIAPWLASHTDKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            33224..34084
                     /gene="kbaY"
                     /locus_tag="ROI21_001349"
     CDS             33224..34084
                     /gene="kbaY"
                     /locus_tag="ROI21_001349"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDX5110294.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            34251..34727
                     /gene="agaB"
                     /locus_tag="ROI21_001350"
     CDS             34251..34727
                     /gene="agaB"
                     /locus_tag="ROI21_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDX5110295.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            34766..35569
                     /gene="agaC"
                     /locus_tag="ROI21_001351"
     CDS             34766..35569
                     /gene="agaC"
                     /locus_tag="ROI21_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDX5110296.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            35559..36350
                     /gene="agaD"
                     /locus_tag="ROI21_001352"
     CDS             35559..36350
                     /gene="agaD"
                     /locus_tag="ROI21_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDX5110297.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            36351..37118
                     /locus_tag="ROI21_001353"
     CDS             36351..37118
                     /locus_tag="ROI21_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDX5110298.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYMALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTHLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(37168..38031)
                     /gene="rsmI"
                     /locus_tag="ROI21_001354"
     CDS             complement(37168..38031)
                     /gene="rsmI"
                     /locus_tag="ROI21_001354"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDX5110299.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEDDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            38096..40132
                     /gene="lpoA"
                     /locus_tag="ROI21_001355"
     CDS             38096..40132
                     /gene="lpoA"
                     /locus_tag="ROI21_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDX5110300.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEDKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQTLSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKILPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPAATTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            40090..40485
                     /locus_tag="ROI21_001356"
     CDS             40090..40485
                     /locus_tag="ROI21_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDX5110301.1"
                     /translation="MATVPTRSSSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            40505..41095
                     /gene="diaA"
                     /locus_tag="ROI21_001357"
     CDS             40505..41095
                     /gene="diaA"
                     /locus_tag="ROI21_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDX5110302.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            41105..41680
                     /gene="yraP"
                     /locus_tag="ROI21_001358"
     CDS             41105..41680
                     /gene="yraP"
                     /locus_tag="ROI21_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDX5110303.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(41793..42833)
                     /locus_tag="ROI21_001359"
     CDS             complement(41793..42833)
                     /locus_tag="ROI21_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDX5110304.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVIAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGIAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(42906..43541)
                     /locus_tag="ROI21_001360"
     CDS             complement(42906..43541)
                     /locus_tag="ROI21_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDX5110305.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            43669..44187
                     /gene="yhbO"
                     /locus_tag="ROI21_001361"
     CDS             43669..44187
                     /gene="yhbO"
                     /locus_tag="ROI21_001361"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDX5110306.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGS"
     gene            complement(44167..44610)
                     /locus_tag="ROI21_001362"
     CDS             complement(44167..44610)
                     /locus_tag="ROI21_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDX5110307.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGMQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            44660..44962
                     /gene="yhbQ"
                     /locus_tag="ROI21_001363"
     CDS             44660..44962
                     /gene="yhbQ"
                     /locus_tag="ROI21_001363"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDX5110308.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKKLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(44949..45452)
                     /locus_tag="ROI21_001364"
     CDS             complement(44949..45452)
                     /locus_tag="ROI21_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDX5110309.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCCWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(45446..45970)
                     /locus_tag="ROI21_001365"
     CDS             complement(45446..45970)
                     /locus_tag="ROI21_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDX5110310.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLAIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            46179..47174
                     /locus_tag="ROI21_001366"
     CDS             46179..47174
                     /locus_tag="ROI21_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDX5110311.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            47183..48061
                     /locus_tag="ROI21_001367"
     CDS             47183..48061
                     /locus_tag="ROI21_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDX5110312.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            48142..49149
                     /locus_tag="ROI21_001368"
     CDS             48142..49149
                     /locus_tag="ROI21_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDX5110313.1"
                     /translation="MTDKTIPFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(49267..50511)
                     /gene="mtr"
                     /locus_tag="ROI21_001369"
     CDS             complement(49267..50511)
                     /gene="mtr"
                     /locus_tag="ROI21_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDX5110314.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     IFGVGNALVHILSSFNLLPVYQ"
     gene            complement(50665..52554)
                     /gene="deaD"
                     /locus_tag="ROI21_001370"
     CDS             complement(50665..52554)
                     /gene="deaD"
                     /locus_tag="ROI21_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDX5110315.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(52547..52627)
                     /gene="yrbN"
                     /locus_tag="ROI21_001371"
     CDS             complement(52547..52627)
                     /gene="yrbN"
                     /locus_tag="ROI21_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDX5110316.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(52734..53618)
                     /gene="nlpI"
                     /locus_tag="ROI21_001372"
     CDS             complement(52734..53618)
                     /gene="nlpI"
                     /locus_tag="ROI21_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDX5110317.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLRLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(53727..55862)
                     /gene="pnp"
                     /locus_tag="ROI21_001373"
     CDS             complement(53727..55862)
                     /gene="pnp"
                     /locus_tag="ROI21_001373"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDX5110318.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(56109..56378)
                     /gene="rpsO"
                     /locus_tag="ROI21_001374"
     CDS             complement(56109..56378)
                     /gene="rpsO"
                     /locus_tag="ROI21_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDX5110319.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(56527..57471)
                     /gene="truB"
                     /locus_tag="ROI21_001375"
     CDS             complement(56527..57471)
                     /gene="truB"
                     /locus_tag="ROI21_001375"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDX5110320.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FDTEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEVHCSKGTYIRTIIDDLGEKLDCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(57471..57872)
                     /gene="rbfA"
                     /locus_tag="ROI21_001376"
     CDS             complement(57471..57872)
                     /gene="rbfA"
                     /locus_tag="ROI21_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDX5110321.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
BASE COUNT        14548 a        15315 c        14235 g        13839 t
ORIGIN      
        1 gggttttctg tgcacaacaa aaaaaggcac cttacgaggt gcctctgata attagctaag
       61 tgcatgttgt tcatgtcgga tgcggcgtga acgccttatt aaacccacaa aacgtttata
      121 ttcaatatgt tacaatgacc gcgtaggcca gataagcgta gcgcatctgg cgattttgcg
      181 cttgccctta tccttcacag cgccttatta attcatcgcg ataatattta ctgccatttc
      241 attcacgtct attcttaatt tgctgcttta aaaacaagtg attgagcaaa attgaggcaa
      301 aaatggactg gtatctgaaa gtactaaaaa attatgtcgg tttccggggt cgtgcgcggc
      361 gcaaagagta ctggatgttt attctggtca acatcatctt tacgttcgtg ctggggctac
      421 tggataaaat gttaggctgg caacgtgccg gtggcgaagg catcctgacg acaatctacg
      481 gtattctggt gtttttacca tggtgggcag ttcagttccg ccgcctgcac gacaccgacc
      541 gctcggcgtg gtgggcactg ctattcttaa tcccgtttat cggctggctt attatcatcg
      601 tctttaactg ccaggcgggt acgccaggcg aaaaccgctt tggacctgat ccaaaactgg
      661 aaccttaaaa caaatctact cgccggatgt ctgactcatc cggcttttat cattatgttg
      721 atgaatgaat aatttactaa tgtttattta aaatatttca atgtcgttat ataaattaca
      781 tataaaaaat aaccatgtat ttatcttaaa tatcaaaacc tttataacat ccgaaaaaga
      841 aagatacgaa tagcatttac acaatacccg gaataaactt tcgtcacttt taattaaagg
      901 gatgttttta tgcagtggta tttatccgta ttaaaaaatt atgttggttt ctctggtcgc
      961 gcacgtcgta aagagtactg gatgtttact ctgattaacg ccatcgtcgg cgctattatc
     1021 aatgtcattc aattgatttt aggtttagag tttccgttcc tttctctcat ttacctggca
     1081 gcaacaatca ttccggtcat tgctctgtgc gttcgtcgtc tgcatgatac tgaccgttca
     1141 ggtgcgtggg cgcttctcta tctggtaccg atcattggct ggttagttct gtttgtattt
     1201 gcctgtttag agggcaattc tggcagcaat cgttacggaa acgatccgaa gtttggttca
     1261 aattaatctt aaaattgggg cgatatttcg ccccttttta ttaacaataa caaatattat
     1321 tttccgctaa aaagtttggg aatttcccgc agacaccagg atttcgcttc ccccatactg
     1381 tcacgacgcc aggccataat aatatcgatc tcgctagtcg attctgggct gacgacacgc
     1441 aaccgccctt ccgcgatatc tttttcgacc atcggatacg gcatcgtcgc cacgccaagc
     1501 cccgccagta atgcctgacg tttatcttca atcgtgctca ccgttaaacg cggctgtttg
     1561 tccagtagct gtacggtcaa taccgggcgc tcacgagcgg tatccgccac cgcaattcca
     1621 cgatatttca cgcgcgtcac ttcagataac ggttccggct cctgatgaat cgggtgatca
     1681 ggcgcggcaa cgtagacgtt cattaacgta tagagcttgc gcgagttgat ctccgacgag
     1741 gaacgaaaat gcatatccgg cgcgataacg atatccgccc gcccctgctc cagtcgttcc
     1801 cacgcccccg ccagcacttc ggtgatgatt gccagttggg tattggcttt tgccgccagt
     1861 ttgtcaatta acgggaaaaa ggcaggtgtc ggtaccagcg cttcggtcac aatggtgaga
     1921 tgcgtttccc aaccgcgcgc gagagcttcc gcatcggtag tcagtttatc tgcggcttcc
     1981 agcaaaacgc gccctcgctc cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg
     2041 cccgagcggt caaacagcac cacatcaagc tcttcttcca gtttttgcat ggtgtagcta
     2101 agtgcggaag gcacgcgtcc cagctcatcc gccgccgccg caaaactgcc ccggcgatcg
     2161 atcgcatcca taacccgtag tgcttccagc gttaatgccc tttctttggc catttcgttc
     2221 tcattcagga aatttgaaca taccgggcag aatatctggc taacaatgta gcgtccagcc
     2281 ccttaccata aaaggaagta aagagaggtc aagaattatg attactaccc gaactgccag
     2341 gcagtgtgga caagcagact acggatggtt gcaggcccgg tatacttttt cctttggaca
     2401 ctacttcgac ccgaaattgt taggctatgc ctccctgcgc gtgcttaacc aggaagtgct
     2461 ggccccaggt gccgcctttc agccgcgaac ctatcccaaa gtcgatattt taaatgtgat
     2521 tctggatgga gaagctgagt atcgcgacag cgaaggcaat catgttcaga ccagcactgg
     2581 tgaggtgttg ctgctctcta cccagccggg tgtcagctat agcgaacaca atctcagcaa
     2641 agacaaaccg ttaacgcgaa tgcagctttg gctggatgcc tgcccacaac gagagaatcc
     2701 gctgattcaa aaactggcgc ttaatatgag caagcagcaa ttaatcgcct cgccagaagg
     2761 tgggatggga agcctgcaat tgcgccagca agtgtggctg caccatatcg tgctcgacaa
     2821 aggcgaaagt gcgaatttcc agttgcatgg gccacgcgcg tatttgcaat cgattcacgg
     2881 gaaatttcat gcgcttacgc attatgaaga gaaagcggcg ctgacctgcg gtgatggggc
     2941 gtttattcgt gacgaggcta acattacgct ggttgccgat tccccactgc gcgctttgct
     3001 gatagatttg cctgtctagt tgtttttaca gggagatgat gatgagtaaa aaatcggcca
     3061 aaaagcgtca gccagtgaag cccgtggtgg cgaaagaacc tgctcgcacc gccaataatt
     3121 ttggctatga agagatgttg agcgagctgg aagccatcgt cgcggatgct gaaacgcgtt
     3181 tagccgagga tgaagctacc gcgtaaagac aggtgccgga tgcggcgtaa acgccttatc
     3241 cggcctacaa aacccaacaa catcaacaaa ttgtgcaatt cgtaggccgg ataagatgcg
     3301 ccagcatcgc atccggcacg atacccaaaa cctggcgttt atctggcctt gctcgccata
     3361 atctcgataa tctgccgatc cgtttgctgc atcgaatggc ttgctaacgc acacaggttg
     3421 gcaatcgact gctcaacatc atgcgccaca atcccttcat tgccggtcac tgcggtatca
     3481 tccagcgcca ttaacaccgc tttccacgca gccgaagcac tggtcgaaac cttcatcgcg
     3541 cagctgttcg acgctccatc gcaaatcatg ccgctgacat cgccgatcat actgctgatc
     3601 gccatcgaaa tggtttcata acgcccatcc accagccatg ccatcccggc ggcggccccc
     3661 attgctgcgg tcgttgcggc acaaagcgca gacaaacgcg gtaactggtt atggatgtaa
     3721 atcgcgctca aatgcgaaag catcagcgca cgcgccagcc gttcatcatc ggccccgaag
     3781 tgttctgcta ccaccaccac aggcattgtt gcagtgatcc cctggttacc cgagcctgaa
     3841 ttactcatcg ccggaagcgt agcaccgccc atacgcgcat cggatgccgc gctggtacga
     3901 atcacaatgg atgaagagag atctttcgcc agcaaaccgc gcgcgcactg tttttccagc
     3961 gtcgcaccaa tatgcagccc ccagttaccg ctcaaacctt cctgcgataa cgcacaattt
     4021 aacttcgcag aatcgagaat aaagcggatc gccgcaaacg ggacttcatt gacgaacttc
     4081 aggatctcag ccagcgtcgt cctggaaagc accgtcagcg gcgactcttg ctcgccctct
     4141 gtcacacacg cctgctgggt aaacaccaca ccattgtgcg tctcaatatg cacaatgttg
     4201 gtatgcccgc cgacaatggt gacacacgcc cacttctcac cgttccagac tttggcgcgt
     4261 gagaagagga tttcatcgca aggttcctgg atcttaactg agactttccc cgccgccagc
     4321 agtgctttgg catcggcaat tgcctgcgca gttgcatctt tcagcacttc cagcccggcg
     4381 ttagcatttc cacctaacgc tcccagcgcc gccgcaatcg gcagccccac cattcccgtg
     4441 ccgggaacgg tgacgcccag accgttcttc atcagatttg gcgaaaccca ggcttctaca
     4501 cgttcaaccg gaccttccag ttctgccgca gcaaccgccg ccgccagcgc cagtgaaatc
     4561 ggttcagtac accccagcgc cggttttacc tcctcctgaa cggcgaggat gtaacgctgc
     4621 cataacggat ttaaagtcga atcaaacata ttaaaaacct taaaatttca ggtaaatcaa
     4681 gaaaatgcca ggaacggaga aacacaaagc aacaaaccag tgacgataat caggtacaga
     4741 gacatccctt tgtatttgtg caatgccggt actttgtaaa ccagccacgc cgggatcagg
     4801 caccctacca tgccgaaaat cgggctacag atagaggtga agctcaacac cggggcgttc
     4861 agtacgatgg cgctccaggc cagcaaaatg gcgaaaatca tgatgccgcg ctgaacgaga
     4921 ttttcgttaa tcttctcggc aggtatcttg cgacgcagga tgttcattac gatcccttgc
     4981 gttgcttcgc gaaagcctaa atagacgcca aagaatgcag tcatgacagc aaagatattg
     5041 agaatgacgc tgaccacttt cacccacgct gcgccgtcac cgctaataaa ctgtgcggca
     5101 atcgccaacg cggaaatatt ctgctcgtag gcttttaccg cttcgtcatg tcccatcgcc
     5161 agcgtgaacg acacggcgta gaaaaagacg gtgacaaaca aaatgccaaa cgcgatattc
     5221 attgcccgca atgctttatg ccgcgccact tcaattgatt tttcccgcga gcgataagag
     5281 atcaccatcg ggcttaacgt ctggataaac agaatcgacg tcagggtaaa cggcagcgta
     5341 ataatggcgt ttttcaccag cagccccagc ggcggtagcg aaccgacgtt atacagatgc
     5401 cacattccca ccatcgacac gcccagcgcc gcgaccacca gcagcttggt cagcaccatg
     5461 ccggtcgaaa ttttaaataa caatttctcg ccgcgtgagg agatcgccac cagaatgcaa
     5521 atcagtacca gaccataaaa gggactgtct gacagcaacc cttccgtcac gccgaaggta
     5581 tgcaggtagg aagcactatc gttggtgatg gcggtggaat agacgaacat ccaaatcacc
     5641 agcattacga aatagagcgc acctaacagg atgccccagt ttttacctaa ataaccgcta
     5701 atgacactcg ggtaatcttt acattctggt gattctgcca gcgtattaat aaacaatcgc
     5761 tgaaacagat acattgccgg gtaaccaatc accgatgaga gcaaaaatac ccacaatccc
     5821 atcaaaccga cctgcaccgg gagaaaaaca atccccgcgc caatcgccat cccgatactc
     5881 ataatcaccc agccggtgtc agtactgtcg aatttgatcg cttctcgcca ctcgctctca
     5941 ctcattccag cacgacccgc cggggtcgaa gcgtctgcaa tgacgccttt attcgatgca
     6001 atttccataa tttctcgctc aatattttgt agggcttatt atttttttcc gagccgcatc
     6061 aaggcgatat gcggtctacg tttttgcagg cgagttatta gaataaagaa atgatacgcg
     6121 cgaggtcaga gaaaatcttc acaatcaaga ctttgaaata acggagatgg ataagaattt
     6181 tctactaaat taatcacaag agaatattca ttctctattt gcggcagatc acaaaaaaaa
     6241 ggtgcacatt tgtgcaccca aggatgaaag ctgacagtaa tgtcagccgc agaccacttt
     6301 aatggccagt cctccgcgtg atgtttcgcg gtatttatcg ttcatatctt tgccggtttc
     6361 atacatcgtc tcgatcactt tatcgagtga aacacgtggt gccgaggtgc ggcgcatcgc
     6421 catccgtgcg gcatttactg ctttcacggc attaatggca ttacgttcaa tgcacgggat
     6481 ttgtacctgt ccggcaaccg gatcgcaggt cagcccaagg ttatgctcca tcgcgatttc
     6541 cgccgcattg catacctgcg ccggactgcc gcccagtagt tcagttaacc ctgccgccgc
     6601 cattgaacag gccacgccaa tctccccctg acagccgact tccgcgccag agatggaggc
     6661 gttcatttta tacagcgcgc caatagctcc cgcggccaga aaatagcggg caattgaccg
     6721 ctcgtttacc ggacgacgga acttatcgta ataagccagc actgccggaa taatgccgca
     6781 cgcaccgtta gtcggtgccg ttaccacacg cccgccagct gcgttttctt cactaaccgc
     6841 cagcgcgtac atattgatcc agtcgatgac attcatcgga tcgttagaga tgttatcgct
     6901 ggaaaccagc tgacgacgca gcgctacggc acgacgcggc acattgagcg gaccaggcag
     6961 cacgccttca gtgttcatcc cgcgttcaat accgtcatgc atcacttgcc agatacgggc
     7021 aaaccctgcg tcaatttccg ctttgctgcg cagcgccagc tcgttgtgca tcatcagacc
     7081 agatatagac aggccgttgt aatcacacat tttcagcagt tcacctgctg agtggaaatc
     7141 gtaaggtacg gacgtttcga catcgtgcga caggccgaag tgttcttctt cgacaataaa
     7201 cccgccgccg acagagtaat aggttttact taatagcgct tcctgtccct tccaggcagt
     7261 gatccgcatt ccgttctcat ggcgaggcaa catttcggga tggaagataa tgttctttgc
     7321 tacaggaaaa tcaacaatat gcgcaccaga tgccactggc agccgcccgc tgcgcgttac
     7381 taactctata aatgcaggga tctcatcaat gacaacatcc tgcggactgt ttcctgccag
     7441 tcccatgatg atggcgacat ccgtggcatg gcctttgccc gtcagtgaca acgacccgta
     7501 cagatcgacc acaatatggc tcgtcgcggt taataagccg ctactttcca gccgatcaat
     7561 aaaacttttt ccggcattca ttggccccac ggtatgcgaa ctggagggac caatcccaat
     7621 tttgaaaata tcgaatgcac taatcatatc cacaccctcg gattgccgtt cagtgaagtg
     7681 gagcggaacg accttgcgac cgtcccgctc acgaggcttt acgcactacg tactgcgatg
     7741 gcttcaattt ccagcttcac atctttcggc aaacgcgcga cctgtacaca gctccgggtc
     7801 gggtaggtcg cctgatgctc atcgaagaac tgcttataga cttcgttgat ggtggcaaaa
     7861 tcattcagat cggtgatgaa cacggtcatc ttgatgatat cgcccacgct cagcccggca
     7921 gcaaccacga tcgctttgac gttttcgagg cttaaacgcg cctgatcttg cacatcagcc
     7981 gggatctcac cggtctgtgg gcaaaccgga atttgcccgg aggtgaagac catgctgcct
     8041 aaatcaacgc cctgaacata agggccgatt gcgcctgggg cacgttgcgt ttcgataatc
     8101 tttttcatac atcctccggc gtcagagcgc ctgggtaaaa gtacgtgaaa taacatcctg
     8161 ttgctgttca cgggtcagtg cgttgaagcg cacggcgtag ccagagacac ggattgtcag
     8221 gttaggatat ttttccgggt gctcgatggc atccagcagc atttccctat tcattacgtt
     8281 gacgttgagg tgttgaccgc cttcgacatc cgcttcgtgg tggaaatacc catccagcag
     8341 gccgacaagg ttggttttac gtactggatc ttctttgccc agcgccgcag gcacgatcga
     8401 gaaggtgtac gagatcccgt ctttggcgta ggtgaacggc agtttcgcca ccgacgtcag
     8461 cgaggctacc gcacctttgc ggtcacgacc gtgcatcggg ttagcgcccg gcgcgaacgg
     8521 tgttccggcg cgacgaccgt ccggcgtatt accggttttc tggccgtaca ccacgttaga
     8581 agtgatagtc agaatcgact gggtagggac ggcgttgcga taggttggca gcgctttaat
     8641 tttcttcata aagcgttcaa ccagatcgca ggcaatgctg tctacgcgct cgtcgttgtt
     8701 accgtactgc ggatattcgc cgtcgatttc aaagtccacc gccaggccgt tttcgtcacg
     8761 gattggtttc acgcgggcat atttgatggc agacagggag tccgtcgcca ccgacaggcc
     8821 cgcgatgccg catgccatag tgcgatagac atcacgatcg tgcagcgcca tcagcgaagc
     8881 ttcgtagctg tacttgtcgt gcatgtagtg aatgatattc agcgcactga tgtactgcac
     8941 cgccagccag tccatgaagt gatcgagact gtccatcact ttgtcgtaat ccagcacgtc
     9001 gtccatcagc ggtgccgttt tcggaccgac ctgaatcttc agcttctcat ccaccccgcc
     9061 gttaattgcg tacagcagcg ttttcgccag gttagcgcgt gcaccaaaga actgcatttg
     9121 cttaccaatc accattgggc tgacgcagca ggcaatcgcg taatcgtcgc tgttgaagtc
     9181 agtacgcatc agatcgtcgt tttcatactg caaggaagag gtgacgatcg acacctgcgc
     9241 ggcatatttt ttgaaggcaa tcggtaattc ttccgaccaa agaatggtca ggttaggttc
     9301 cggtgccggc cccatagtgt gcagggtgtg caaatagcgg aaggagtttt tggtcaccag
     9361 cgtacgaccg tccagcccca tcccgccgat cacttccgtc gcccagattg ggtcgccgga
     9421 gaacagcgaa tcaaattccg gtgtacgcag gaagcgtacc atacggatct tcatgatgaa
     9481 gtgatcgatc agttcctgtg cctgctgctc attgagtacg ccagctttaa agtcgcgctc
     9541 aatgtagata tcgaggaacg atgccgtgcg ccccagcgac atcgcgccgc cattttgcga
     9601 tttcactgcc gccagataag cgaagtagag ccactgcacc gcttcctgcg cattctgcgc
     9661 cgggcgagag atatcaaagc catatttcgc cgccatttcc tgaatctgca acagcgcatg
     9721 acgatgctct gccagctcct cacgcagacg gatagtggct tccagatcct cgcctttttc
     9781 cagacgggac tggagatcgg caaattgcag ttcgcgttca cgtaccagat aacgaatgcc
     9841 atacagcgcc acgcggcggt agtcaccaat aatgcgccca cggccataac catctggcaa
     9901 accggtcagt acgccagatt tacggcagcg cagcatatcc ggtgagtaaa catcaaatac
     9961 gccctgatta tgggttttac gcagatcggt aaacagatat tcaaattcac tgtccatttc
    10021 acggccatag gcgtggaatg aacttttaat catattaatg ccaccgaacg gatgcagcgc
    10081 acgctttaat ggcgcatccg tttgcaggcc cactattttt tccagcggct ggttaatata
    10141 tcccgcatca tgagcggtaa tggtggtagc aatattagta tcgaaatcaa ccggcgcgtg
    10201 ggttgcgttt tcgatacgaa tgccttccat caccttttcc cacaattccg tggtggctgg
    10261 cgtcgcttcg gcgagaaaag attcatcacc ttcatacggt gtatagttat gttgaataaa
    10321 atcgcggaca ttaatttcgt ttttccagtc cgtaccttta aagccaagcc atgcgtcggc
    10381 gtacagctta tcgctggtat caatatctac cttcatgaaa aataatctct ctacaatact
    10441 tcaactaaat tatgcaaatt ctgcgggcgc gttcactttg cctaaatgaa tggcatccaa
    10501 agcaatcatt ttttcttcgt tggttgggat aacggcgcaa atgacacgcg cattttcact
    10561 ggaaacaatt cgctctccac aggagttaga gcgattattc atttctgtat caatctctac
    10621 gcctaataca gccaaatgtt ccatgaccag acgacgaatt aaacttgaat tttctcctat
    10681 tccgccggtg aatataattc catccaggcg acgtaatgag gctgcgtgtc cggcaatatg
    10741 acgggcaatt cggtgaacaa aggttttaat tgccagttgc gcgcgttcgt gaccttcatg
    10801 ccaggctttt tccagaacac gtaaatccga agaaagaccg gaaataccta ataatcccga
    10861 ctctttattc actacgcgtt ccaggtcacc caggctctgg ttggtttggc tggcgaccca
    10921 ggacatcgcc ccgaagtcga catcaccact gcgggtaccc atcatcaagc cttccagcgg
    10981 cgtcattccc attgaggtat caacactctg accgttgcga actgcgcaga ttgacgcgcc
    11041 attgccaaga tgcgccacaa ccaggccgga gtcatcttcc gccagattca gcaaagaatg
    11101 cgcgcgctgg gaaacatagc ggtgcgacgt gccgtggaaa ccataacggc gtacacctaa
    11161 ctcttcataa tatttccacg gcaggccgta taaataagct tccggagcca tcgtctggtg
    11221 gaaactggta tcaaataccg ctacctgagt tacgccagga aataattgct gcgccgattc
    11281 aataccactt aaattggcgt aattatgcag gggtgccagt ggagaaacgc gacggatatt
    11341 atcaatgact tcatcggtaa taatggcgga ctcggtaaaa atactgccgc cgtgagcgat
    11401 gcggtggcca attaaggcca cactgtcatt taaattccgt ttttccagtt caaatgcaat
    11461 tgccttcaat gcaccttcgt agctgtggtg agccagcggt gctggctctc ccccatttac
    11521 ggataagaat gcattttccg agttaatacc gtcggcaata cctgacatta atacttcaca
    11581 gtcgctagca tcgagcacgg agaacttaat cgaagacgaa ccacagttaa taaccaaaac
    11641 aaccggaaat tcattcatct cttttctcat cctgagttac ggattaaaac agtttgtata
    11701 cgatgttcag gatggtcagc agaccaatca cggtaacaaa cacgttatcc agacgaccac
    11761 ggtatttcgc cagagacggc gctttacgaa tggcatacat cggcaacagg cacagcagtg
    11821 atgcgataat cggtgcgccc atggcttcaa tcaggtcgag gatgttcggg ttggcgtagg
    11881 caacaaccca ggtggagccc atgatgaaga tcatgctgag ggtattcagt ttacccagcg
    11941 acactttggt tttgtcacct ttataaccga acttcagaat caggccattc aagccttcca
    12001 gcgtccccag atagtgaccg aagaaagatt tgaagatagc cacgagtgcg atgatggaag
    12061 ccgcatattc cagtgtaatc gcgaacgttg ttttggtacc ggtcatggac gcaaagtggt
    12121 tagccagata agaaagcact ggaatattct gcgctttggc ttccgccatg ttggccggag
    12181 acagcgtaaa caggcagcta aaggcaaaga acatcaccac tgcaaccatc agcatgctgg
    12241 cacgagaaat gatttgggag catttacgtt cggtgaagtc gcgcccgaag tctttctcat
    12301 actcttcacg tttagaaacc acgaaggaag agacgattgg cgagaagtta aaggagaaaa
    12361 ccatgatgga aatccccagc cagacagtga tcaggatacc gtcatgaccg gttaacgaca
    12421 gcgaaccgag gtcaacctgg tcgataactg cagagttcca gtaagggatc agcgacagag
    12481 aaatcagcac caggctggcg ataaacggcc ataccaggta gctcatcact ttaaccatca
    12541 gatccttacc aaaccagatg acgaaagcca tcagcagcaa caggaacagc gccacaaagc
    12601 cgcgattcag cggtgcaaag ccgagctggt tttcccagaa cgtcataaag gtattggtaa
    12661 tagtaacgcc ataaatccac agcagtgggc aaatcgcgaa gaagtacagg aacgtgataa
    12721 ccacgccgcc tgttttacca aaatgctctt ccaccgtttc cgtaatgttg ccggaagggt
    12781 tagagccaga aagacacaga cgcgccagcg cccggtggca ataaaatgcg atggggtatg
    12841 ctaataccaa catcagaaga atcgggatca gtccgccaaa acctgcgcgg atagggaaga
    12901 acagcacccc ggcgccgatt gccgtaccaa acaagcctaa cgtccatgtg gtatctgatt
    12961 tacgccagga cgattgtttt gtctggctgg atacaatgct atctgaagta ctcatatcct
    13021 atcctcaacg aattaattaa gcgtcaacga aaccggtgat ttgagagacg cgagaaagat
    13081 cgatattgcc gccggaaata atactgacgg tttttctgtt ttgaatatat tggtctaatt
    13141 taccgcttaa taatgcagca catgccagag cgcctgcgcc ttcggtgacg actttattgc
    13201 gctgaattaa ggcaatcata ctgtttctga tttcatcttc gctgaccagc acgatgtcat
    13261 cgactaattc acgaacgatt tcgtaagtta aattacccgg gcgggagaca tcacaaccat
    13321 ccgccagggt gccggtagtt cggtgcgtgg ttatttctcc ggagtggaaa gaagccgcca
    13381 tgccgtgaac gttttctgac tgtacgccaa taacacgaat ggtcgggtta atagatttta
    13441 ttgccaccgc aataccagca attaaaccgc caccaccaat tggcacaatc acattatcga
    13501 catcatagag atcttccata atttccagac caatcgttcc ctggccagca atcactttcg
    13561 gatcatcata aggtgggata aaaatacggc cttccatttc gacaatttcg ctcactttag
    13621 cgatagtgtc gttgaagtta tcaccatgca gaacgacttc tgcagagtag tcgcacgttg
    13681 ccgctacttt ggatttcggc gcgccttttg gcatcaccac tttaccgtcg atacccagca
    13741 tcgcgcagga gagcgaaacc ccttgcgcat ggttgcccgc agagcaggcc actacacctt
    13801 tacgtttttc cgcatcggtc agtgaactta atttattaaa tgcgccacga attttaaatg
    13861 aaccggtacg ctgcatgttt tcgaatttga ggaatatttc acctttgcaa cgttcactaa
    13921 aatagttgga gcgaggcatg cctgttttat aaattcgccc agccagtcgt tgttttgctt
    13981 caatgatgtc atcaatagca accgggagat cgtatgtaat atgcattata aaacctctta
    14041 gtctgaatta aatatgtagg taaccgtaac cgacacctgc aaaacagaca ggtggattat
    14101 ttatgtttaa ataaaaacaa ataactaacc aacttcaatt aattgccttc gtctacaccc
    14161 attataagat gaatactctt tggctaattc caccaaaacc gatgctgctt ttttaatacg
    14221 ataatttttc gaccataccg cggcatattg tgccacaggt aatgtttctt caaccggaat
    14281 agtaataaat tgatttgaac caaaaggtga tgtcatatca caaggaatta cagttaagaa
    14341 atcagcattg agaacaagat tataaattgt cacgactgag tcggttttaa cgatgttttc
    14401 aatactgatg ccatttcttt gtaacgtagt aagtagctcg ctgtagtacc ccatattggt
    14461 ttgtggcaac acccactgtt cgttcttcaa cgactccagc gtggtggtac cggtgcatgt
    14521 tcgggactta ctggctacca gcacaaactc ggactcgaac agcggctcaa catgtaaatc
    14581 ctgaagcttc atttctgcgc ttaacgtacc aatcgcaaaa tccagtcgac cgtcgcggat
    14641 tgccggtaag aacgaagaca gttgcgcttc atacatagaa acctgcgctt tcgggaacac
    14701 ctctttgaac ttgttgatca tccctgacat aaaagtaaaa ccaatcaatg aaggaaaacc
    14761 aaatgagact tccaccaccg cctcagaaga cataccgctt atctcattaa ccatattttt
    14821 catttcacgg gtaatggatt cggaacggga gagtaacaat tgaccggcag gtgttaatgt
    14881 tacaccggta tttttccgca ccactaattc cacgccaaaa taatcttcaa tatcgttaat
    14941 gattttactg acggccggtt gagttaaccc taattctttt gcagccgagc cgatagagcc
    15001 acttctaatg acttcctgaa agactaccag gtgctgcgtt ttcggaagaa gaatagtgct
    15061 cataacgacc tacgttaatt acctcattga cggcatgaag tgtatcaaaa tgaaatgaac
    15121 aggatatgtg cgaccactca caaattaact ttcaatactt ttcagggtat cgttattcca
    15181 attaaatatc tttatatatc aataagttaa agtataaaaa tcagcataaa ccctgatttt
    15241 tgtcaaaaaa aatgacgggg ataaccatat tttatggcga taacattatt cgttatgtgg
    15301 ttaatacaaa aaaggggctg agaccatatt tcaagcaatt accggataat taccaggcaa
    15361 attttgagtt aaatttaatg agcaagttct caaattttat tataaataaa tcattttcag
    15421 cgataaataa tgcggcacgt cacatttttt cacgctattt gttggagaac aaacatttat
    15481 tttatcaata ttttaaaatt tcgaatacag gtattgatca tctcgaacaa ttgattaacg
    15541 tcaacttttt ctcttctgac aggacgtcat tttgtgaatg caatcgtttt ccataaattc
    15601 ttctcccctc ataggtgacg aatagcattt tgtgttgagg atcacaaaac gaataattgc
    15661 tggtcgccgc gataaggtca gccaaagaca acaaggggaa ttttcacaga gcttttgatc
    15721 ggcgtaggcc acagaatgtt gcttcgttta ctgtaatgcc gggtaaatga gcgttttttg
    15781 atagtgcgaa agtgccgtgc gaggaaaata agcatctatt attgtttgtc atgatgacaa
    15841 ggcaggcatc aatgaaaggc ttcccaattg cacatatttt tcacccttca atcccgccaa
    15901 tgcacgcagt ggttaacaat cacaacagaa atattgatta ttggacggta aaaagaaagt
    15961 ttgcagaaat tgtctccacc aatgacgtta ataaaatata cagtataagt aatgaactgc
    16021 ggagagtatt atctgcaatt actgcattga acttctatca aggcgatgtt ccttctgtca
    16081 tgattcgaat ccaaccggaa aatatgagtc cattcatcat agatatttct acaggagaac
    16141 atgacgatta tatcatacaa acattagatg taggcacttt tgcacctttt ggcgaacaat
    16201 gtacttgctc agccgtcaat aaaaaagagc tggagtgtat taaggagacg atttctaagt
    16261 attgtgcaaa attcacccga aaagaagcca tcttaacccc cccctacgta ctttaataaa
    16321 accgccattc cttcagattg ttggcaaatt ctcttttttt cacccgatca ttttaataat
    16381 gatttttatt gattatttat gaaggataaa ataatgtccc ctgtttcatc aattggtaat
    16441 gatattagta gtgatttagc ccgtcgaaaa atgaatgacc ttcctgaaag ccccatagga
    16501 aataacctcg aagcactagc accaggcata gaaaaactaa aacagacctc tattgaaatg
    16561 gtcactttac ttaacgcgtt acaaccaggt gggaaatgca ttatcactgg tgattttcaa
    16621 aaagaattag cgtacttaca aaatgtaatt ctttataatg actcctctct tcgtatggat
    16681 ttttttggtt ataacgcccg aattattcaa cgatcagaca atacctgtga acttacaatt
    16741 aatgaaccgt taaaaaacca ggaaatatcc acaggcaata tcaatgttaa ttgcccattg
    16801 aaagacattt acaatgaaat caggaggttg aacgtaatct ttagttgtgg gactggaggt
    16861 atagttgatc tatcctctct ggacttacgg aatgtcgatt tagaacttta tgattttaca
    16921 gataaacata tggccaatac aattttaaat ccttttaaat tggatgacac agattttact
    16981 aatgccaaca tgtttcaggt aaatttcgtt agttcaaaac aaaacgccac aatctcatgg
    17041 gattatttac taaaaataac gcctgttata acaagcatta acgatatgta ttctgaagaa
    17101 aaaattaagc ttgtcgaaag ttgtttaaat gcgcttggag atattaccga agaacaatta
    17161 aaaattatga gacttgcaat tatagaatct ataccaagag caactctaac agatgaatta
    17221 gaaaatgaat taacaaaaga aatatataaa agctcatcga aaattaacaa ttacttgagc
    17281 agaattaaat taccagagat gaaagcattc tcattagaaa aaatagatta ttacatcgat
    17341 ataatcatta aagattatga aagcgtcaaa gaaaatgctt atctaatcgt tccaaaaatt
    17401 aattacaata cagatttaaa catagaggac tctagctcag aggaattcct tccagataat
    17461 acaatcgaga aagacgaaaa ttctccagac aattgctttg aagtcgtaaa atataataca
    17521 tatgaagctt ataactcaga gaagcaatat tataccagag aggactatac gtatgattac
    17581 gaccttttaa atgcaatata gacttaataa cttcttacaa agtctattat taataccgta
    17641 gatattcatc ttaccctgtg caccaaaagc aaaaacttat cgacgcgggt ttgaaactga
    17701 ttgcaaccgc aaaaatgcct gatgcgctac gcttatcagg cctacgccat ctctgcaata
    17761 tattgaattt gcgtgctttt gtaggcagga taaggcgttc acgccgcatc cggcatgaat
    17821 aaagcgcact caacaatctg aaacccgcca gtttacgctg ccgggttttc gttaaaagag
    17881 gtgaaactga ccaataagcc gctttctttt gagtatagtg tcgtggacag tcattcatct
    17941 ttctgcccct ccaaaagcaa aaacccgccg aagcgggttt ttacgtaaat caggtgaaac
    18001 tgaccgataa gccgggttct gtcgtggaca gtcattcatc taggccagca atcgctcact
    18061 ggctcaagca gcctacccgg gttcagtacg ggccgtacct tatgaacccc tatttggcct
    18121 tgctccgggt ggagtttacc gtgccacgga ctgttaccag ccgcgcggtg cgctcttacc
    18181 gcaccctttc acccttacct gatcccgctt gcgcgggcca tcggcggttt gctctctgtt
    18241 gcactggtcg tgggtttccc ccccaggcgt tacctggcac cctgccctat ggagcccgga
    18301 ctttcctccc ctccgcccgt ctcccccgaa gaggacgacg acgaagcggc gactgtctgg
    18361 tcagcttcgg cgcgcagtat agagggtttg cgcgcccttg tcaccccgcg ttgcgcattc
    18421 caatcgccag tgtcgcggcg atattacgtg aagcacggta aatattgtca taagccccgc
    18481 ggaatgcttc gtccaacgta ccaatactgg tcaatacgct gaagaccgca tcaatgccat
    18541 gctgatgtac aacgccaaca tcattggtca ggctacccgc aatgccaatc accggtttat
    18601 tgtacttttt cgccacgttt gcgacgccaa tcggaacctt gccgtgtata ctctggctgt
    18661 caatacgccc ttcaccggtg atcactagcg tacaatcgtg aatatgttcc tccagattca
    18721 gcgccgtagt gacgatttca ataccacttt tcagttccgc accaagaaac gccattagcg
    18781 ccgcgcccat accacccgca gctcctgcgc cggggacatc tttcacatca acatgcagcg
    18841 cttttttaat gacatcggca tagtgagaga ggttattgtc cagctcaaca atcatcgctt
    18901 cactggctcc cttttgtggg ccaaagatgc gcgatgcacc gttatcgccc accagcggat
    18961 tggtgacatc acaagcgacg cgaatgacgc aatcttttaa gcgcggatcg aggccggaaa
    19021 tatcaatatc attcagagta ttaagactac cgccgccaaa accaatttca ttgccgttgg
    19081 cgtcgcataa tttcgccccc agcgcctgta ccatgcctgc accgccatca tttgtagcgc
    19141 taccgccaat gccgataata atgtttgtcg cgccgctctc cagcgcctgc aggattaact
    19201 cgcctgtgcc gcgtgaagtg gtcacgagtg gatcgcgttt ttccgcaggt accagctcca
    19261 gcccactggc cgccgccatt tcaataaacg cggttttgcc atcgccggag atcccccaac
    19321 tggcattcac cttctcgccc agcggccctg taacccaggc gtgacgttcg gaaccctggg
    19381 tggctgcaat catcgcttcc accgttcctt cgccaccgtc ggcaaccgga acagaaacgt
    19441 actgtgcatc aggaaaaatt tcccgaaatc ctttttctat cgcctgcgca acctcgctgg
    19501 cagataaact ttctttataa gagtctgggg cgattacgat tttcatacct atgcctgtta
    19561 ccacatgacg ccggagggcg tttctcttat tcggcctgga ttccaggccc ggattgcaat
    19621 acgccatccg ggcacgacgt cattaacgag taacttcgac tttcgccagt ttttcgtagt
    19681 agcacgccag ggcgctatga tctgccgttc ctaaaccatc tgctcgcact gcctgcatca
    19741 tctccataac cgcagctgtg agcggcagtt gcgcgccgac gccgtgggaa gtatccagcg
    19801 catttgccag atccttaata tgcagatcaa tacggaagcc cggcttgaag ttgcggtcca
    19861 tcaccatcgg cgctttggca tccagcacgg tactgcccgc cagtccaccg cgaattgcct
    19921 gatacaccaa atccgggtta acgcccgctt tggttgccag cgttaacgct tctgacatcg
    19981 cggcaatatt cagcgccaca atgacctgat ttgccagttt ggtgacgtta cctgcaccga
    20041 tttccccggt atgcaccacg gaacccgcca tcgctttcat caaatcatag tatttgtcga
    20101 aaatagcctt gtcgccgccc accatcactg acagcgtacc gtcgatagct ttcggttcac
    20161 cgccgctcac cggagcatcc agcatatcaa tgcctttcgc tttcagcgct tcgctgattt
    20221 cacggcttgc cagcggtgcg atagaactca tatcgatcaa taccgtacct ggcttcgcgc
    20281 cttcaataat gccattctca cccagcgcca cctctttcac atgaggggag tttggtagca
    20341 tggttatgat gacgtcgcac tgttcagcga tcgctttagc cgtagaggct gtttctgcac
    20401 ctgcagcaat cacgtcagca atagcttctg ggttacggtc agcaaccacc agcgagtaac
    20461 ctgctttcag aaggttttta ctcattggtt tacccataat ccccaggcca aaaaaaccaa
    20521 ctttcatagt catatcaatc atctctcttg ttgcggtggt ggttattttt taaaggtatc
    20581 agccagtttc tgagtggcag agcggaagac gccgagatcg ctgccgacag ccacaaacgt
    20641 cgccccccat tccagataac gacgcgcatc ggcttcgacc ggtgcgagga taccgctggg
    20701 tttgccgtgc gcactggcac ggttaaaaat atgctgaatt gctttttgta catccgggtg
    20761 cgaggcattg ccgagatggc ctaatgccgc agccagatcg ctggggccga cgaagatacc
    20821 gtctacgcct tcggtagcgg caatggcatc gacgttatct acgccctgct gactttctat
    20881 ctggaccaga atggtgatgt tcttgttcga ctgagcgaaa taatccgcca cggtgccaaa
    20941 catattggcg cggtgagaaa cggagacgcc gcgaatgcct tccggtgggt aacgggttga
    21001 tgccaccgcc tgctctgctt cctcttttgt ttctacaaaa ggaatcagga agttatagaa
    21061 accgatatcc agaagacgct taataattac cggctcgttg gtcggcactc gcactactgg
    21121 cgcgctggcg ctgcctttca gggccattaa ctgcggaata aacgtggaga tatcgtttgg
    21181 cgcatgttcg ccatccagca ccagccagtc aaacccagcc aaaccaagaa cttcagtgct
    21241 aatcgggtta gagagtgctg accagcaacc aatttgtacc tgtttcgcag ccagtgcggc
    21301 tttgaattta ttcgggaaaa catcgttatt catcgcttat acctttgctt atttctgcaa
    21361 ttccatacgt ttaatgtcgc caactacaaa gaggtagcag accatcgcca tcagcgctga
    21421 acatcccacg aaaaccagtg ctgcattgaa ggagtgcagt tcacttacca gatagccaat
    21481 caccagtgga gtgacaatgg aggcaacatt gccaaagacg ttaaagacgc cgccgcagag
    21541 gccaacaatc tctttcggcg cggtgtcaga aattaccggc cagcccagcg caccaaatcc
    21601 tttgccaaag aaagccagcg ccatcagcat gaccaccagc gtggtgttgt tggtgtagtt
    21661 acataagatg atggtggaag ccagcaacat tcccagcaca atcggtagct tacgtgccag
    21721 ggtcagggac aaaccgcgtt tgatcagata atccgagaag acacctccca gcacgccgcc
    21781 cgcaaagcca cacagtgccg gaatcgaggc gaccagaccc actttcagaa tcgacatgcc
    21841 tttttcctgc accagataaa ttgggaacca ggtgaggaaa aaccaggtga tggtgttgat
    21901 aaaatattgt ccgaaaaata cgcccagcat catgcggtta gagagcaatt gcttgatgta
    21961 atgcagtttg ggaccgcttg ctgccgcact gcccggcttt ttatggtcca tatcgaccac
    22021 cgcgccattt tctgagataa acttcagctc ttccgcagac atacgtgggt gatctgtcgg
    22081 gttatgaatc aacttgatcc acagcgccgt cagcacaaaa ccaattaccc ccataacggt
    22141 aaagacgtgc tcccagcccc aggcgaaagt cagccagcca agcagcggcg aaaagagcgc
    22201 cagcgagaaa tattgcgccg agttaaagat ggcggaggca gtaccacgtt ctttcgtcgg
    22261 gaaccaggcg gcgacaatac gggcgttcgc cgggaatgat ggcgcttccg agaaccccag
    22321 cataaagcgc ataaagaaca tggagatccc tgcccaggcc aacgggaaca tatcaacaaa
    22381 gccttgcagg aaggtgaaca gcgaccagaa aaagaggctg taggtgtaaa cttttttcga
    22441 gccaaactta tcaagcaacc agccgccggg gatttgcatc agcaggtagg cccagccaaa
    22501 agcggagaag atgtaaccca tcgaaaccgc acttaactgc aactcttttg ccacttcggt
    22561 accagcaata gacagcgttg cacgatcggc gtagttaacg gcggtaacaa taaaaataat
    22621 cagtaatatt aaatagcggg tatgcacgcc tttctttttt acgtcaacgg tgtctagaat
    22681 cattttattt acctcgggta cttatgctga tttttattat tatggggaag gtgttattta
    22741 tgagttttat ttatgccgtt acggcaatga actcgggaat tagtataagc agcgcgagaa
    22801 taataatcat tgtgcaaatg ctaatttaat taatactatt taaatattat tttgagcata
    22861 tgcacataag gttgcgcgct aaagcacaga tttgcgcttt accttaccgt gcggcactgc
    22921 aatccctgaa atgattgaca ttgatcacat ttctgcgttt aaactcctga cattcttatt
    22981 tcacccaatg aagtcattta tttttaaatg agaccaggtc ctcattttaa taacccctgg
    23041 ctggagaata ttgcacaatg gccaacatcg aaatcagaca agaaacgcca actgcgtttt
    23101 atataaaagt tcacgacaca gataatgtgg caattattgt taatgataat ggcctgaaag
    23161 caggaacgcg ttttccggat gggctggaat taattgaaca tattccccag gggcataaag
    23221 tcgcattgct ggacattccg gctaatggtg aaattattcg ttatggcgaa gtgattggtt
    23281 acgccgtgcg cgcaatccca cgcggaagct ggatcgacga atcaatggtt gtactaccgg
    23341 aagcgccgcc gttacacacg ctgccactgg caaccaaagt cccggccccc ttaccgccac
    23401 tggaaggata cacctttgag ggctatcgca atgccgatgg cagcgtgggc accaaaaacc
    23461 tgctcggcat caccaccagc gtccactgtg tggcaggcgt ggtggactat gtagtaaaaa
    23521 tcattgaacg cgatctgcta ccgaaatacc cgaacgtcga tggcgtggtg gggctgaatc
    23581 atttgtacgg ctgtggcgtg gcgattaatg caccggcggc agtggtgcct attcgtacca
    23641 tccacaatat ttcgctgaac cctaactttg gcggcgaagt aatggtgatt ggtctgggtt
    23701 gtgaaaagtt gcagcctgag cgcctgctga ctggaacgga tgatgtgcaa gctattccag
    23761 tagaaagcgc cagcattgtc agtttgcagg atgaaaagca tgtcggcttt cagtccatgg
    23821 tcgaggatat tttgcaggtc gccgaacgcc atttgcacaa actgaatcaa cggcagcgag
    23881 aaacctgtcc ggcttcagaa ctggttgtcg gcatgcagtg cggcggcagc gatgcgtttt
    23941 ctggcgtaac ggcaaacccg gcggttgggt atgcgtctga tctactggtg cgctgcggcg
    24001 caacggtgat gttttcagaa gtcacggaag tgcgtgacgc gatccatctg ctgacaccac
    24061 gcgcagtgaa cgaagaggtc ggcaaacggc tgctggagga gatggagtgg tacgataact
    24121 atctcaatat gggaaaaacc gaccgcagcg ccaacccttc gccgggcaac aagaaaggcg
    24181 gtctggcaaa cgtggtggag aaagcgctcg gctccattgc taaatcgggt aaaagtgcaa
    24241 ttgttgaagt gctgtcgccc ggtcaacgcc cgactaaacg cggattaatt tacgccgcga
    24301 cgccagccag cgattttgtc tgtggcacgc aacaggtggc ttcagggatt accgtgcaag
    24361 tgtttacgac cggtcgcggt acgccgtacg gcctgatggc ggtacccgtc attaaaatgg
    24421 cgacccgcac cgagctggcg aaccgctggt ttgatttaat ggatattaat gcgggcacca
    24481 tcgctaccgg cgaagaaact attgaagagg tgggctggaa gttgttccac tttattctcg
    24541 acgtcgccag cgggaagaag aaaaccttct cagatcaatg gggactgcat aaccagctgg
    24601 cggtgtttaa cccagcaccg gtgacctgat ttcttttgga acgtccctcg caaaacatgg
    24661 ccttagtgcc atgtttttat tgtttaaagt ccccacgtcc attaataatg catttgcatt
    24721 accttagttc aagcttataa tttgagcaga aaacaggctg taaaaggaca gtgaatcatg
    24781 cccgctaatg ctcgctctca cgctgtactg accactgaat caaaggtcac gatacgcgga
    24841 caaacaacta tccccgcgcc agtgcgtgag gccttaaaac tgaagccagg ccaggacagc
    24901 attcattacg aaattctgcc tggtgggcaa gtatttatgt gccgactggg agatgaacag
    24961 gaggatcata ctatgaatgc atttttgcgt tttctggatg cagatatcca gaacaacccg
    25021 caaaaaactc gtccattcaa cattcaacaa ggaaagaaac ttgtcgctgg catggacgtc
    25081 aacattgatg atgagattgg cgacgacgaa taatggattt tccacaaagg gttaatggtt
    25141 gggcgctata tgctcatccc tgttttcagg aaacctacga cgctttagtt gccgaagtcg
    25201 agacattaaa gggaaaagat cctgaaaatt atcagagaaa agccgccaca aagttattgg
    25261 cggtagtcca taaagtgatt gaggagcata tcacggtcaa tccatcatca ccggcattcc
    25321 gtcatggcaa gtcgttaggc tctgggaaaa ataaagactg gtcacgggta aaatttggtg
    25381 ctggtcgtta tcgtctcttc tttcgttata gtgaaaaaga gaaagtcatc attctgggat
    25441 ggatgaacga tgaaaacact ctgcgcacct acggtaaaaa aacagatgcc tataccgtat
    25501 tcagcaaaat gttaaaaaga ggacatcctc ctgccgactg ggaaaccctc acccgagaga
    25561 cagaagaacc ccattgatgg tgttcacaat gcccgccgaa cgcattcgtg ttgaccagcg
    25621 ggcacccttt ctcactcccc gaccagaatc acttcaaccc cagcctttcg cagcccttcc
    25681 aggctatccg caggaatgcc ttcatcaaca atgatcatgt cgatacgttg agtatcaatg
    25741 atcttatgta aactggaacg attgaattta ctggaatcgg tgaccacgat gatccgttcc
    25801 gcaacttcgc acatccggcg gtttaaacgg gcttcatctt cattatgcgt actgacgccg
    25861 cgctccagat cgatcgcatc tacgccaaga aacagcatat cgaagtggta attttgcagc
    25921 gattgttcag cctgatcgcc gtaaaaagat tgcgactgac ggcgcaaatg cccgccggtc
    25981 atcagcagct caacgccttc cgcttccagc aacgcattag ccacgttcat accgttggtc
    26041 atcgcaatta cgtcagtgtg cttgcgcatc agacgagcaa tctcaaaagt ggtggtcccg
    26101 gaatcgagga tcacccgatg acctggctga atcaactcaa cggcagcttt cgcaacgctg
    26161 cgtttcatcg cggtgttcag cgcgctttta tcttccactg atggctcgac tgacggcgtg
    26221 gtgctatcgc agatcaacgc gccgccatag gcacgcacag cgatcccctg cttttccaga
    26281 aacgccagat cgttgcggat cgtcacagta gatacgccat acaatgccga cagatcgtta
    26341 acctgcacac tcccttgctg tcgcagacgc tgaatgatct gttctcgtcg ctcgctggtg
    26401 cctgtcattc gcttctcacc tgaagcgtcg gtattactca tagtaagtcc tttcgtaaaa
    26461 ctttcgtttc atttcgtttt gcctattaac gcctttctat taagcaaatg caagcccacc
    26521 ttacccattg gcgcaagcta ctctcgtttc agtggctttc attatgtttc ttttgtgaat
    26581 cagatcagaa aactattatc tttcgtttta tttttatctc accatggcgc agtatcaact
    26641 gaaacaaaac gaaagattaa tatcgcagta atctgaactg gagaggaaag tgaaacatct
    26701 gacagaaatg gtgagacagc acaaagaggg caaaacaaat gggatttatg ccgtttgttc
    26761 cgcacatccg ctggtgctgg aagctgcaat ccgctacgcc agtgcaaacc aaacgccgtt
    26821 actgattgaa gcaacctcca atcaggtaga ccagttcggc ggttataccg gaatgacgcc
    26881 cgccgatttt cgcggctttg tttgtcagct cgccgactcg ttgaatttcc cacaggatgc
    26941 gttgattctg ggtggcgacc atctgggtcc aaaccgctgg caaagcctgc ctgccgctca
    27001 ggcaatggcc aatgccgatg atttgattaa aagctacgtg gcggcaggat tcaaaaaaat
    27061 tcaccttgat tgcagcatgt cctgtcagga cgatccaatt cccttaactg atgacatcgt
    27121 ggctgaacgc gccgcacgtc tggcgaaagt ggcggaagaa acctgtcttg aacactttgg
    27181 cgaagccgat ctggagtatg tcattggtac cgaagtaccg gtacctggcg gcgcgcatga
    27241 aaccttaagc gagctggcgg tcaccacgcc ggatgccgcc cgcgccacgc tggaagccca
    27301 tcgtcacgcc tttgaaaagc aaggtttgaa tgccatctgg tcacgcatca ttgccctggt
    27361 ggttcaaccc ggcgtcgaat tcgatcacac caacgttatt gattatcagc ccgccaaagc
    27421 gagcgcctta agccagatgg tcgaaaacta cgaaacgctg attttcgaag cgcactctac
    27481 cgattatcaa acgccgcaat cgctgcgcca gctggtgatt gaccactttg ccattctgaa
    27541 agttggcccg gcgctgacct tcgccctgcg tgaagctctg ttctctctgg cggcgattga
    27601 agaagaactg gtaccagcga aagcctgttc cggtctgcgt caggtgctgg aaaacgtgat
    27661 gctcgaccgc ccggaatact ggcaaagcca ctaccacggt gacggcaacg cacgtcgcct
    27721 ggcgcgtggt tatagctact cggatcgcgt gcgctattac tggccggaca gccagattga
    27781 tgacgctttc gctcatctgg ttcgtaatct ggcggattca ccaattccgc taccgctcat
    27841 cagccagtat ctgccgctgc aatacgtgaa agttcgctcc ggcaagctgc aaccgacgcc
    27901 acgggaactc attatcaacc atattcagga catcctggcg cagtaccaca cagcctgtga
    27961 aggccaataa gcaaaacaaa gaggaacacg ctatgccaaa tattgtttta agccggattg
    28021 atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg ggggcaaatc
    28081 tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac ctgatggaaa
    28141 tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtc atcgacaaca
    28201 ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacacct gccgatttcc
    28261 tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat atgcactacg
    28321 ccaatggcaa gcaacaaatc gccaaaacgg tttctgtgga tgcgggcgat atcgcagcat
    28381 ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg acagagcctg
    28441 ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagtct gttgcaggca
    28501 ttcgcgttgg gcattatcgc ctttatcgct ggcctggata tgtttaacgg cttaacccat
    28561 atgcaccgcc cggtggtcct cggcccattg gtcgggctgg tacttggcga tctgcatacc
    28621 ggtattttaa ccggcggtac gctggaactg gtgtggatgg ggctggcccc gctggcaggc
    28681 gcacaaccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc cattactact
    28741 ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc agtacagatg
    28801 gggattacct tcctgttctc ggtgatgtct ggcgtgatgt cccgctgcga ccggatggcg
    28861 gagaacgccg atacacgcgg cattgaacgc gtgaactatc tggcattgct ggcactcggc
    28921 actttctatt ttctctgcgc tttcctgcct atctacttcg gcgcagaaca tgccaaaacc
    28981 atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg cggcatcatg
    29041 ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta catcccctac
    29101 ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac ctgtgctggc tattgctgcc
    29161 gccgcgctgg cgatggcgct gatcgacctg ctgcgtaaat ctcctgaacc gactcaacct
    29221 gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc cgaacgtcag
    29281 tgaaaacgaa gaaacactgc tgactggcgt caatgaaaac gtgtatgaag atcagagcat
    29341 tggcgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca tgctgttaca
    29401 agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc tgctgcctgc
    29461 tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaaag ggcatatggg
    29521 tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc ttgcgatgga
    29581 gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg gtgcgccgct
    29641 cggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg gcgggatagg
    29701 cgcaagcctc gcgctacaag gctctattct tggcgctgtc gtctttattg tgctgttcaa
    29761 cgtggtgcac ctggggctgc gttttggtct ggcgcattat gcttaccgca tgggcgtggc
    29821 ggcgattcca ctcattaaag caaataccaa aaaagtcggc catgcggcat ctatcgttgg
    29881 gatgacggta atcggcgcgc tggtggcaac ctatgttcgt ttaagcacca cgctggaaat
    29941 caccgcgggc gacgcagtgg ttaagttaca ggctgatgtt atcgacaaac tgatgccagc
    30001 cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg gctggagtcc
    30061 gctgcgcctg attgcgatta ccgtggttct cggcatcgtc ggtaaattct gccatttcct
    30121 ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg cggatttgcc
    30181 agcggcatgg aaaaagcaat gaagcaaatc ctcggcgagc aatcgcagtt tattgccatc
    30241 gattttcccg aaacctcaag caccgcgctg cttacatcgc agcttgaaga ggccatcgcc
    30301 caactcgatt gtgaagacgg cattgttttt ctgacggatt tgctgggcgg cacgccgttt
    30361 cgcgtagctt caacgctggc gatgcaaaaa ccgggctgtg aagtgatcac cggcaccaat
    30421 ttgcaactgc tactggagat ggtgctggag cgcgaagggt taagcggtga agagttccgt
    30481 gtgcaggcgc tggagtgtgg acatcgtggg ctgaccagtc tggtggacga gttaggccgc
    30541 tgccatgaag aatgtccggt cgaggaagga atatgacaca cgttctgcgc gccagaaggc
    30601 tgctgactga agagggatgg ctcgatgacc atcagttgcg tattgctgac ggtgtaatcg
    30661 cagcaatcga accgattcca gtgggcgtga ctgaacgcga tgcggaactg ctctgccccg
    30721 cttacatcga cacccatgta catggtggtg cgggcgttga tgtaatggat gacgcgccgg
    30781 atgtgctcga caagctggca atgcacaaag cacgcgaagg tgtcggcagt tggttaccga
    30841 ccaccgtaac cgcgccgctt aataccattc atgcggcgct gaaacgtatt gctcaacgtt
    30901 gccaacgcgg cggacctggt gcgcaagtgc tggggagtta tctcgaagga ccgtacttca
    30961 cgccgcagaa taaaggcgcg catccgccgg agctgtttcg cgagctggaa attgccgagc
    31021 tggatcaatt gattgccgtt tctcagcaca ccttgcgcgt ggtagcgctg gcaccggaaa
    31081 aagaaggggc attgcaggcc atccgccatc ttaaacagca aaacgtacgg gtgatgctgg
    31141 ggcatagcgc ggcgacctgg caacaaaccc gtgccgcgtt tgacgctggt gccgacggcc
    31201 tggtgcattg ctataacggg atgacaggtt tacatcaccg cgaaccggga atggttggcg
    31261 cgggattaac ggacaagcgc gcctggctgg aactgatagc cgatggtcat catgtgcatc
    31321 cggcggcgat gtcgctgtgt tgttgctgtg cgaaagagag aatcgtgctg atcaccgacg
    31381 cgatgcaggc agccgggatg ccggatggtc gctatacgtt atgtggcgaa gaagtgcaga
    31441 tgcacggtgg cgttgtccgt accgcgtccg gtggactggc gggcagtacg ctgtctgttg
    31501 atgcggcagt gcgcaatatg gtcgagttga cggacgtaac gcctgcggaa gccatccata
    31561 tggcgtcgct gcatccggcg cgtatgctgg gtgttgatga tgttctggga tcgcttaaac
    31621 cgggcaaacg cgccagcgtc gttgcgctgg atagcgggct gcatgtgcaa caaatctgga
    31681 ttcagggtca attagcttcg ttttgatggt ttgctccttt attgggcctt cacttccccc
    31741 gtaaggcctt tctttttctt tctttttgat ctgtgcagcg gtgtcggatg cgacgctgac
    31801 gcgtcttatg cgacctacgg tacgccgcat ccggcaatcg gtgcacatag caacgtcatc
    31861 atgcaattct ttaatcttcc cttgtctttt ctttcaacga tcacaaattt cgttttattt
    31921 cttttttctc cattgaactt tcagtttctt ttctatagat tttaatcaac gaaagacatc
    31981 accaaatgaa atgaaacgaa aggtaagcga aagcgacaac gcccgacgtc aagttcatca
    32041 gactaaggat tgagttatgc cagaaaatta cacccctgct gctgccgcaa ccggtacatg
    32101 gactgaagaa gagatccgcc atcagcctcg cgcatggatc cgttcactca ccaacatcga
    32161 cgcgctacgt tccgcgctca ataacttcct tgaaccgtta ctgcgcaaag agaatctgcg
    32221 ggtaatcctg accggagccg gaacctcggc atttatcggt gacatcatcg cgccgtggct
    32281 cgccagccat accgataaaa acttcagcgc cgtaccgacc accgatctgg tcaccaatcc
    32341 gatggactac ctgaatccag ctcatccact gctgttgatc tccttcggtc gatccggcaa
    32401 cagcccggaa agcgtcgccg ccgtggaact ggcaaatcaa tttgtacccg agtgctatca
    32461 cctgccgatc acctgcaacg aagcgggcgc tctttaccaa aacgcgatca acagcgacaa
    32521 cgcgtttgcc ctgctgatgc ccgcagaaac gcacgatcgc ggctttgcga tgaccagcag
    32581 cattaccacc atgatggcca gctgcctcgc ggttttcgca cctgagacga tcaacagcca
    32641 gagcttccgc gacgtggcgg atcgttgcca ggcgatcctg acctcactgg gcgatttcag
    32701 cgaaggtgtg tttggttacg caccgtggaa acggatcgtt tatctcggca gcggtggctt
    32761 acagggcgca gcacgcgagt cggcgctgaa agtactggaa ctgacggcgg gtaaactggc
    32821 ggccttttat gattccccga ccggattccg tcatggcccg aaatcgctgg tcgataacga
    32881 aacgctggtg gtggtatttg tctccagcca cccttacacc cgccagtatg atcttgatct
    32941 gctggctgaa cttcgtcgtg acaaccaggc aatgcgcgta atcgctatcg ccgcggaaag
    33001 caacgacgtt attaccgccg gtccacatat catcctgccg ccgtcacgtc actttatcga
    33061 cgttgagcag gcattttgct tcctgatgta cgcccagacg tttgcactga tgcagtcgct
    33121 gcacatgggc aatacgccgg ataccccatc agccagtggc accgttaacc gcgtggtgca
    33181 aggcgtaatc attcatccgt ggcaggcata agaggatcgc attatgagca ttatctccac
    33241 caaatatctg ttacaggacg cccaggccaa tggctacgcg gtgcctgcct ttaacattca
    33301 taacgccgag acgatccagg cgatcctcga agtgtgcagt gaaatgcgat cgccggtgat
    33361 cctcgccgga acgccgggga cctttaaaca tatcgcgctg gaagagatct acgccctgtg
    33421 tagcgcctat tccacaacct acaacatgcc actggcgctg catctcgacc accacgaatc
    33481 gctggatgat attcgccgta aagtccacgc aggcgtgcgc agtgcgatga tcgacggcag
    33541 ccacttcccg tttgccgaga acgtaaagct ggtgaaatcg gttgtcgact tctgccactc
    33601 acaggattgc agcgtggaag cagaactggg ccgcctgggc ggtgttgaag atgacatgag
    33661 cgttgacgcc gaaagtgcat tcctgaccga tccacaagaa gctaaacgct ttgtcgaact
    33721 gactggcgtc gacagcctgg cggtagcgat tggtacggcg cacggcttat acagcaaaac
    33781 gccgaagatt gatttccagc ggctggcgga aattcgtgaa gtggtggatg ttcctctggt
    33841 gctgcatggt gccagcgatg tcccggatga atttgtccgt cgcactattg aacttggcgt
    33901 cacaaaagtg aacgttgcca cagaattaaa aatagccttc gctggcgcgg ttaaagcctg
    33961 gtttgcggaa aatccgcagg gtaatgatcc tcgttattat atgcgcgtcg gtatggatgc
    34021 gatgaaagaa gttgtcagaa ataaaattaa tgtctgtggt tcagcgaatc gaatttcagc
    34081 ataatcatta gttttttatg atttatccca atgtacttcc cgattaatat cggggagtgc
    34141 cttaatggta aaggagataa ctaaacctta ataaatacat cagtacaata ccgcaacaat
    34201 aatatattta aaaagattat attattcaac tttatggtga ggattacaca atgagcagtc
    34261 caaatattct cttaacccgt attgataacc gtctggttca cggtcaggtt ggcgtgacct
    34321 ggacatccac catcggtgcg aatctgctgg tagtcgtgga tgatgttgtc gctaacgatg
    34381 atattcaaca gaaattaatg ggtattaccg cagaaaccta cggctttggc attcgtttct
    34441 ttactatcga aaaaaccatt aacgtcatcg gcaaagctgc accgcatcag aagatcttcc
    34501 tgatttgccg cacgccacaa acggtacgta aattggtaga aggtggcatt gacctgaaag
    34561 atgtcaacgt cggcaatatg catttctcgg aagggaaaaa gcaaatcagc agtaaagttt
    34621 atgtcgatga ccaggatctc acggacttac gttttattaa acaacgtggc gtgaatgttt
    34681 tcattcagga cgtccctggc gatcaaaaag aacaaatccc tgactaaatc taaaatcgcc
    34741 ttaatattgg tttgaggtaa taaaaatgca tgaaataacc ctacttcagg gattatccct
    34801 ggcggcgtta gttttcgttc tggggattga tttttggctg gaagccttat ttttattccg
    34861 cccgataatc gtttgtaccc taactggcgc tattctcggt gatattcaga ctggcttaat
    34921 taccggtggt ctgacagagt tggctttcgc cggattaacc cctgcaggtg gtgttcagcc
    34981 gcccaacccg attatggcgg gtctgatgac cactgtcatt gcatggtcta cgggcgttga
    35041 tgccaaaacg gcaattggtc ttggcctgcc gtttagtttg ttaatgcagt acgtcattct
    35101 gttcttctat tccgctttct cattatttat gaccaaagcc gataaatgcg caaaagaggc
    35161 ggatacggca gcgttttccc gacttaactg gacaacgatg ctcatcgtcg cttcagcgta
    35221 tgcggtgatt gctttcctct gtacttacct ggcacagggg gcgatgcagg cgctggtgaa
    35281 agcgatgccc gcctggctga cccacggctt tgaagtggct ggcggtattc tgcctgccgt
    35341 tggttttggc ttgctgctgc gcgtgatgtt caaagcgcaa tatatccctt acctgatcgc
    35401 cggtttcctg tttgtttgct acatccaggt cagcaacctg ttgccggttg ccgtactggg
    35461 cgcaggcttt gcggtgtatg agtttttcaa tgcgaaatcc cggcagcaag cgcaaccgca
    35521 gcccgttgcc agtaaaaatg aagaagagga ctacagcaat gggatctgaa atcagtaaaa
    35581 aagatatcac ccgtctgggc tttcgttcgt cactgctgca agcgagcttt aattacgaaa
    35641 ggatgcaggc gggcggtttt acctgggcga tgttgccgat cctgaaaaag atttataagg
    35701 acgacaaacc gggcctaagc gcggcgatga aagataacct cgaatttatt aatacccacc
    35761 cgaatctggt cggattcctg atggggttat taatttcgat ggaagaaaaa ggagaaaacc
    35821 gcgacaccat taaaggcctc aaagtagcac tgtttggccc aatcgccggg attggcgatg
    35881 cgattttctg gtttacctta ttgccgatta tggcgggaat ttgctcatca tttgccagcc
    35941 agggaaactt gctggggccg attctatttt tcgccgttta cctgcttatc tttttcctgc
    36001 gcgtcggctg gacccacgtc ggttattcag tcggcgtgaa ggcgatcgat aaagtgcgag
    36061 agaactcgca gatgattgct cgctcggcaa ccatcctcgg gatcacggta atcggcgggc
    36121 tgatcgcttc gtatgtgcat attaacgtgg tgacatcgtt tgccatcgac agtacccaca
    36181 gcgtcgcgct gcaacaggat ttcttcgata aagtcttccc gaacatttta ccgatggcct
    36241 acaccctgct gatgtattac tttctgcggg tgaaaaaagc gcatccggtg ctgttaatcg
    36301 gcgtgacttt tgtgctctct attgtttgtt ccgcattcgg cattttgtaa atggaacgag
    36361 gcactgcgtc tggtggtgcc tctttactta aggaatttca tcctgtgcaa acccttcagc
    36421 aagttgaaaa ctatatggcg ttaagtgaac gtgccagcga atatttattg gccgtgatcc
    36481 gtagcaaacc ggatgccgtg atttgcctgg cgaccggagc cacgccatta ctgacgtatc
    36541 attatctggt agaaaaaatc caccagcagc aggttgatgt cagccagctc accttcgtga
    36601 agctcgacga atgggtggat ctgccattaa ctatgcctgg tacctgcgaa actttcctgc
    36661 aacagcatat cgtgcagccg ctggggctgc gtgaagacca gctcatcagc tttcgctccg
    36721 aagagataaa tgagacagag tgcgaacggg taacgcacct gattgcgcgc aaaggcggtc
    36781 tggatttatg cgttctcggg ttggggaaaa acggtcatct tgggctgaac gaaccgggag
    36841 aaagcctgca accggcctgc catatcagtc aacttgatgc cagaacacag caacatgaga
    36901 tgttaaaaac cgcgggccgt cccgtgactc gtgggatcac cttaggcctg aaggatattc
    36961 tcaatgcccg cgaagttttg ttactggtga ctggcgaagg aaagcaggat gcgacagaac
    37021 gttttctcac ggctaaagtc tctaccgcta tcccggcttc atttttatgg ctgcataact
    37081 atttcacatg catgattgat gagatgtgtc gtcgttaact cgcctgctac aggcgttgca
    37141 acgctattcc tgctgcgacg cctgtaatca ctctccctgc tgctccagtg catacttata
    37201 cagcgcattt ttcttcacgc cgtgaatttc tgctgccagc gccgccgctt ttttcagcgg
    37261 cagttctgcc tgtagcagcg ccagcgtgcg cagggcatca gcaggcaggt catcttcctg
    37321 cgctttatgg ccttcgacaa tcagcaccat ttcgcctttg cgacggtttt catcttcttt
    37381 gacccacgcc agcagctcgc caacgggcgc gccgtgaatg gtttcccagg ttttggtcag
    37441 ctcacgcgcc agaacgacgt agcgggattc gcctaatacc gcaacgatat cttccaggct
    37501 atctaacaga cggtgggtag attcataaaa aatcagcgtg cgcggctccg cttcaatggc
    37561 ttttagcgca tcacggcggc cttttgattt ggcaggtaaa aagccttcat aacaaaaacg
    37621 atcggatggt aaacccgcgg cgcttaacgc agtgatagca gcacacggcc cgggtagcgg
    37681 caccacgcga atccccgctt cacggcaggt acgcaccaga tggtagccag gatcgttaat
    37741 tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc ccctcttgca gcttcgccag
    37801 cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc gcaaacaacc gagcattaat
    37861 cccaaaatgt tgcagcaata aaccggtgtg acgcgtatcc tcggcggcaa tcagatcaac
    37921 ggcctgtaat acctccaacg cacgctgggt gatatccgcc agattgccga ttggcgtcgg
    37981 tacaatgtaa agctggccct gagaattatc cgccgattgg tgttgtttca ttgtgtcgtc
    38041 cgtattgccg atttaatatt gagcattgcg taaaaaaaat atcactggat acattatggt
    38101 accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc tggcagccct
    38161 gattttcgcc ggttgtggca cccatactcc cgatcagtcc actgcttata tgcagggcac
    38221 ggcgcaggct gattctgcct tttacctgca acagatgcag caaagttctg atgataccag
    38281 gatcaactgg caattactcg ccattcgtgc actggtgaaa gaagacaaaa ccgggcaggc
    38341 ggttgagttg tttaaccaac taccgcaaga actgaacgat tctcagcgtc gcgagaaaac
    38401 actgctggcg gcagagatta aactggcgca gaaagatttt gctggcgcgc aaaacttgct
    38461 ggcgaaaatc acacctgcag atttagaaca aaaccagcaa gcgcgttact ggcaggcaaa
    38521 aatcgatgcc agccaggggc gtccttccat tgatttactg cgcgcgttaa ttgctcagga
    38581 accgctgctc ggcgcgaaag aaaaacagca gaatattgac gccacctggc agacgctctc
    38641 ctccatgact caggaacagg cgaatacgct ggtgatcaac gccgacgaaa atattctgca
    38701 aggctggctg gatctgcagc gcgtctggtt tgataaccgt aacgatcccg acatgatgaa
    38761 agccgggatc gccgactggc agaaacgtta tccgaacaac ccgggcgcga aaattctgcc
    38821 aacgcagttg gttaacgtaa aagcgtttaa accagcctcg accaacaaaa tcgccctgct
    38881 gttgccgctg aatggccagg cagcggtatt tggtcgcact attcagcaag gctttgaagc
    38941 ggcgaaaaat atcggcactc agccagtggc ggctcaggta gctgccgcac ctgccgcaga
    39001 cgtagcagaa caacctcagc cgcaaaccgt ggatggcgtt gccagcccgg cacaagcctc
    39061 ggttagcgat ctgaccggtg aacaacctgc ggcccagccg gtgcctgtaa gcgccccggc
    39121 ggcaaccacc gcagcggtaa gcgcacccgc gaatccatcc gcagagctga aaatctacga
    39181 tacatcatca caaccactta gccagatctt aagccaggtt caacaggatg gcgcgagtat
    39241 tgtggtcggt ccgttgctga aaaataacgt tgaagagttg ctgaagagca acaccccgct
    39301 gaacgtgctg gcactgaacc agccggagaa catcgaaaac cgcgtcaata tttgttactt
    39361 cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg accagggtaa
    39421 acaagcgccg ctggtgctga tcccacgcag ttcattgggc gatcgcgtag ccaatgcgtt
    39481 tgcgcaagag tggcagaaac tgggtggcgg caccgttctg caacaaaaat ttggttccac
    39541 cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt gctttaacgg gtagcccgat
    39601 tactcccaga gcgacaaccg actccggcat gacgaccaac aatccaacgc tgcaaaccac
    39661 gccaaccgat gaccagttca ccaataatgg cggtcgtgtc gatgcggtgt acattgtggc
    39721 aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg gtagccagag
    39781 cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc accgcaggcc cggatttccg
    39841 tctggagatg gaaggcttgc agtacagcga aatcccgatg ctggcaggcg gtaatctgcc
    39901 gttaatgcag caggcgctca gcgcggtgaa caacgattat tcgctggctc gcatgtatgc
    39961 gatgggcgtc gatgcctggt cgttggcaaa tcatttctca caaatgcgcc aggttcaggg
    40021 ttttgaaatc aacggtaata ccggaagtct gacggctaac ccggattgcg tgattaacag
    40081 gaagttatca tggctacagt accaacaagg tcaagtagtc cccgccagtt aaccaccaaa
    40141 cagaccggcg atgcgtggga agcacaagcg cgtcgctggc tggaaggcaa aggactgcgg
    40201 tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat gcgtgaaggc
    40261 cggaccaccg tttttgtcga ggtgcgttac cgccgctctg cgctttatgg cggcgcggca
    40321 gccagtgtga cccgcagcaa acaacacaaa ttattacaga ctgcccgctt gtggctcgcg
    40381 cgtcataatg ggagttttga tactgtggat tgccggttcg atgtggtagc cttcaccggg
    40441 aatgaggttg agtggattaa ggatgccttt aatgaccact cataattaag gtttaaggat
    40501 tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc attcaaactc aaattgcggc
    40561 ggcagaggcg cttccggatg ccatctcgcg tgcagccatg acgctggttc agtctctgct
    40621 caatggcaac aaaatcctct gttgtggtaa tggaacttcc gctgccaatg cacagcattt
    40681 tgctgccagc atgatcaacc gtttcgaaac ggagcggccc agcttacctg ccattgcact
    40741 aaatactgat aatgttgtct taacggcgat tgccaacgat cgcttacatg atgaagtgta
    40801 tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca tttccacccg
    40861 tggcaacagc cgcgatattg taaaagcggt agaagctgcc gttacgcgtg atatgaccat
    40921 tgtggcatta accggctatg acggcggtga acttgcaggt ttgttagggc cacaggatgt
    40981 ggagatccgc attccttcgc atcgtagtgc tcgcattcag gaaatgcata tgctgacggt
    41041 aaattgcctg tgcgatctga tcgataacac gcttttccct caccaggatg tttaaggaga
    41101 atacatgaag gcattatcgc caatcgcagt ccttctttcc gcgctgctgt tgcaaggttg
    41161 tgttgccgct gccgtagtgg gtaccgctgc tgtaggtacc aaagccgcaa ctgacccacg
    41221 cagtgtcggc acccaggtgg acgatggtac cctggaagtg cgcgtaaaca gcgcattgtc
    41281 gaaagacgaa cagattaaga aagaagcgcg cattaatgta acggcctatc agggcaaagt
    41341 gctgctggtt gggcagtcac caaacgctga actttcggct cgcgccaaac agattgctat
    41401 gggcgtagac ggtgccaacg aagtgtataa cgagattcgt cagggccagc cgattggtct
    41461 gggcgaagcg tctaacgata cgtggatcac caccaaagtg cgttcgcagc tcttaaccag
    41521 cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag tgttcctgat
    41581 ggggctggtg actgaacgtg aagcgaaagc ggcggcagat attgccagcc gggtaagcgg
    41641 cgtgaagcgg gtaaccacag cgtttacgtt tattaaatag cagcccttgt aatgcctgat
    41701 gcgacgcttg ccgcgtctta tcaggcctac aacgatgcaa actgtaggtc gaataacgcg
    41761 tttacgccgc atccgacatg ttttccctca aatcaaacca acagcgccag cccgccgaca
    41821 atcacacctg acactgccac catcgccccc gtcagccata aggctttcgc cgggaacgct
    41881 ttacgcaaca taatcagtga cggcaaactc accgccggga gcgtcatcaa caatgccagc
    41941 gccggagcga ttcccatgcc tgccagcatc atcgtttgta caattggaat ttctgctgcc
    42001 gtgggaatga caaacaagca tcccgctacc gccatcgcaa tcacccacat caggctgtta
    42061 tcgacagcac catcagcatg ggggaataac cagacgcgag cggcacccaa caccagtact
    42121 gcaaggatgt aaaccgggat cgtactccag aaaagcgtcc atagcgccct gccccagcgg
    42181 ctaaaaaatc cgccctgcgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt
    42241 gtttcacgca cccatttttg caccagcgtc gcaatcagca acaccatcac cagccccgcc
    42301 accagacgaa tcgccgcaaa cccccagccg aggacaaacc ccataaacac cagcgtcgcc
    42361 gggtttaaca acggattccc catccagaat gccagcgcac cgcccatcga tacctgttgg
    42421 cgacgcattc ccgccgcgac cggagccgca cagcaggtac acatcatacc cggcagcgaa
    42481 aacagcgttc ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg cagcaaccag
    42541 tcacgcggga tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc
    42601 cataccgcga ggaaatagat catcgcgtaa tccaacgccg cctgccacgg attagcatcc
    42661 gcctgcgcaa ggatagattt accgatactg tgggtttcgg cagcagtaaa cgctttgccg
    42721 tagtaaggtt gccatttcac ataccagaga ccagcaatga caacgagaaa gaaaagcgcg
    42781 ggtttccacc actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc
    42841 cccaggaata agatatgata attgagcgcg tgaatattac gctcgctatc aattcttggg
    42901 aataattatt cagctctttt gcgtaattct gaagagctta aaatcgtcac accttcatgc
    42961 tcaggccgca tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc
    43021 cagttaccgg gtaacaaacg gaatagaggc gcaaaaagcg tttcgttcat ccgctgtttg
    43081 ctacgatcgc ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggcg
    43141 attaatgctt cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcatta
    43201 gcacccatag cactgaccac cagcatatgc tgcgcaccga gccgccgccc ggttaatgcg
    43261 gtatccacca ccagcgtgta atcggcatga ataaacgcct ctttactccc cgcttctcgc
    43321 cgcgtggtgc cgagacaaca aaacacaatg tcgatgggat cggtgacctg cgccagcgcg
    43381 tcagtcagtt gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc
    43441 gtcggcgcgg ctatggcgtt aactttcggt tcgttaatca acatccgcag caggtgaccg
    43501 cccaccagcc ccgttgcgcc tgtaatcagt acctgactca tcttcgctcc tttacagaat
    43561 tgtctgcctt gcgctccacg gctcatgcac caggcttaat agaccatgag gtaattatcc
    43621 ccgattgtgg gaaattcgcc tcatccaatg caacatcgcg gaggaagcat gagtaagaaa
    43681 attgccgttt taatcactga tgaatttgag gattcagaat ttacttcacc cgcagacgag
    43741 ttccgtaaag ccggacacga agtgattacc attgaaaaac aagcgggtaa aacggtgaaa
    43801 ggcaaaaaag gagaagccag cgtggccatc gataaatcca tcgatgaagt gacgcccgca
    43861 gagttcgatg ccctgctgct accgggcggt cattcaccag attatctgcg tggcgacaac
    43921 cgttttgtca ccttcacccg cgattttgtg aacagtggca aacctgtttt cgccatctgt
    43981 cacggtcccc agttgctgat cagcgccgat gtgatccgtg gtcgtaagct gaccgccgtt
    44041 aaaccaatca ttattgatgt taaaaacgcg ggcgcggaat tttacgatca ggaagtggtg
    44101 gtcgataaag atcagctggt taccagccgc acaccggacg atctgccagc gtttaaccgc
    44161 gaggcattac gcctgctcgg ttcctgagtt acgtagccag atcatttttt taccaaagcc
    44221 cagcgtgttg tcggtgaatt tgatttcatc aagccggatt tcccacaccg gggccgacag
    44281 cattctggca accggaaagc gacgattgta cgcctggcgt gcgaggtcgc tttcctcacc
    44341 ttcaagcctg cggatctcgc ctttaaattg catgccgcga attaacgcta ccgttttcgg
    44401 ctgaccgttt accgttccgg caactgccgc ctgcggccca ctcatctgcg cgtggcgcgt
    44461 tttttcttcc gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt
    44521 agcgcaccag agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag
    44581 ccaacggctg atggcggtga gtgtttccat agctgttctc ttttatactg tggcctgacc
    44641 ttaacatgcg acgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa
    44701 taagctttat accgggatca ccacagatgt cgaacgccgc tatcagcagc accaaagcgg
    44761 caaaggggcg aaagcactgc gcgggaaagg agaactaacg ctggcgtttt ccgcgccagt
    44821 gggcgatcgt tcactggcac tacgggcgga atatcgcgtt aagaaactga ccaaacggca
    44881 aaaagagcgt ctggtagcgg aaggcgcagg gtttgcagag ctgttgagca gcctgcaaac
    44941 cccggagatt aaaagcgatt gaagtgctcg tgatactcaa ccagaccggt tacgccgttc
    45001 agcgcgtcat ccgctaaacg atgtacctgg aaggcgcttt cagtgcccgg ccagcagcaa
    45061 cgcagatcgt gatgcgccgc cagctcaaag ccgaaacgac tgtacagcgc cggatcgccc
    45121 agcgtcacca ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt
    45181 tggcgtgcca gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc
    45241 cattgcaggt cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga
    45301 ccttcgtcat ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga
    45361 accagcttcg cttccgcatc actttcgaat gaacgacgca gcagggcatc aatacccggc
    45421 gcatcaatgg gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt
    45481 caggcgcggt tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta
    45541 aggctttcgg catttgctcc agttcaatgg cgtccatcag gtttttcaca tacagcccca
    45601 gctccgtatc gccttcaatc gccagccgac gctggaagaa gagcgtatcc ggatcttgtt
    45661 tacgcgccgc aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg
    45721 cgttctggct aacgaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat
    45781 cacgcacatg aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg
    45841 cctgacggaa ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg
    45901 tcagttttac cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt
    45961 tatccaacac gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa
    46021 ataacatagc ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact
    46081 ttaactgcct taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac
    46141 atttttgcgc tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg
    46201 gaaatctccc ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc
    46261 taaaagatga tactaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc
    46321 aggaagcggt gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt
    46381 ttgcgcatcc ggacggttac gcccgctggc agcgcgccgt ggatatggcg gcgcagctgg
    46441 gtgccgacgc gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc
    46501 cgcatattga acgtcacgta tcggtgcagg cttcggcgac caatgaagag gcgattaact
    46561 tttatcatcg ccattttgac gttgcacgcg tggtgctgcc gcgcgtgttg tcgattcatc
    46621 aggtgaaaca actggcgcgg gtcacgcctg taccactgga agtattcgct ttcggcagcc
    46681 tgtgcattat gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca
    46741 acactgtggg tgcgtgttct ccggcccgtt tcgtgcgctg gcagcaaacg ccgcaggggc
    46801 tggaatcccg cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt
    46861 atccgacgct gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag
    46921 aacccaccag tctcaatacc ctggaactgc tgccggagtt aatggcggcg aatatcgctt
    46981 cggtgaaaat tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcaaaagtct
    47041 ggcgtcaggc tatcgaccgt tgtaaggccg atccgcaaaa cttcataccg caaagcgcgt
    47101 ggatggagac gctcgggtcg atgtccgaag gcacgcagac cacccttggc gcgtatcacc
    47161 gtaaatggca gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc
    47221 aaaagagacg ctggaagaat tttatcagca ggccgccacc agcagcgccg acgtgattta
    47281 tcttggtgaa gcggtctgta gcaagcgccg ggcaaccaaa gttggcgact ggctggagat
    47341 ggcaaaatcg ctcgccggaa gtggtaagca gattgtgctc tccacgctgg cgctggtgca
    47401 ggcatcctcc gaactgggcg aactgaaacg ctatgttgag aacggtgagt ttctgattga
    47461 agccagcgat ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg
    47521 gcacgcgctg aactgctaca acgcggtgac actgaaaata ttgctcaaac agggcatgat
    47581 gcgctggtgt atgccggtgg agctatcccg cgactggctg gtgaatctgc ttaatcagtg
    47641 cgatgagctg ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctgcc
    47701 gctggcctac tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg
    47761 tgaaacctgc tgcattaagt atccgaacgg gcgcaacgtg ctatcgcagg aaaaccaaca
    47821 agtgtttgta ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga
    47881 gctgacatcc atgcaggggc tggtcgatgt ggtccgcctg tcaccgcagg gtactgacac
    47941 tttcgcgatg ctcgacgcct tccgcgctaa tgaaaatggc gcggcaccac tgccgttgac
    48001 ggcaaacagc gattgtaacg gctactggcg gcggctggcc gggctggagc tgcaagcgta
    48061 agtatatagc tcactttgtt aacaacttta actactcttt aatgcagtat taaagattaa
    48121 tcggtaacaa agtgagctgt tatgactgat aaaaccattc cgttttcgct actcgatctg
    48181 gcccctattc ccgaaggttc ttcagcgcga gaagcgttct cccactctct cgacctcgcc
    48241 cgtctggctg aaaagcgcgg ctatcatcgc tactggctgg ctgaacacca caatatgacc
    48301 ggtattgcca gtgctgcaac atcagtattg attggctatc tggcggcgaa taccactacg
    48361 ctgcatctgg ggtctggcgg cgtgatgttg cctaatcact caccattggt gatcgccgaa
    48421 cagttcggca cgcttaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct
    48481 ccgggtagtg accagcgaac catgatggct ctacgtcgtc atatgagcgg cgatattgat
    48541 aatttccccc gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg
    48601 catgtgcgcc cggtaccggg ctatggcgag aaaatccccg tgtggttgtt aggctccagc
    48661 ctttacagcg cgcaactggc ggcgcagctt ggtctgccgt ttgcgtttgc ctcacacttc
    48721 gcgccggata tgttgttcca ggcgctgcat ctttatcgca gcaacttcaa accgtcggca
    48781 cggctggaaa aaccgtatgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc
    48841 gacgctgaat ttctctttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa
    48901 accgggcaac taccgccgcc gattcaaaat atggatcagt tctggtcacc gtccgagcag
    48961 tatggcgtgc agcaggcgct gagtatgtcg ctggtgggtg ataaagcgaa agtgcgtcat
    49021 ggcttgcagt cgatcctgcg cgaaaccgac gccgatgaga ttatggttaa cgggcagatt
    49081 ttcgaccacc aggcgcgcct gcattcgttt gaactggcga tggatgttaa ggaagagttg
    49141 ttgggatagt gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta
    49201 ggactgataa gcgaagcgca tcaagcattg tgtaggcagc agaaatgtcg gataaggcac
    49261 cgctgattac tgatacaccg gcagcaaatt aaagctcgat aaaatatgca ccagtgcgtt
    49321 gccgacgcca aatatcagaa tcagcgcaat catcggcttg ccgccccaga cgcggaattt
    49381 cgggctgcca aagcgtttac gcgatgcgcg ggccaacagc gccggtacaa ttgccgccca
    49441 gatagtagct gctaagccag catagccaat ggcgtacagg aatccgttcg ggaacagaag
    49501 tcccccaaca acaggcgggg caaaggtcag caacgctgtt ttcaagcggc ccatagccga
    49561 gtcatcgaaa ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccgaggaa
    49621 cgaactcgct accgcaaagt ttgagaagac gaccagcagc agatccaggc tacggctgtt
    49681 cagcacgccg cttaacgcct gtaccagcac atcaatatta ccgcccttct cggcgatgcc
    49741 gataaactcc ggacgaggga tgttgcccat cgtcgccagc aaccagatgg tatacagcgc
    49801 cagcgccatc agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta
    49861 atacttcatc aggctcggca cgttaccgtg ataaccaaac gatgccagac agaatggcag
    49921 tgtcatcagc agatacggcg cataagacgc attgctttcg gcgacgttga acaatgtcgc
    49981 aggctgtaca tgccccagca ggctgccgaa ggtgaggaag aaagtaatga ctttcgcccc
    50041 cagcacaatt gctgtcatcc ggctgacagc tttcgtactc aaccacacca cgaacgctac
    50101 cagcagtgca aaaccaaatc cagctgcccg tgcagggacg ttcagcgaca tctccgcgaa
    50161 ggtgtgatgc agaatcgaac cgctggcaga aatataggcg taagtcagga tatagagcac
    50221 aaaggcaatg gaaatgccgt tgactacgtt ccagcctttg cccagcaaat ctttggtgat
    50281 ggtgtcaaag ctagaaccga ttcgataatt caggttagct tccagaatca tcaagccgga
    50341 atgcagcata cagaaccagg taaagatcag cgccgccatt gaccagaaga accacgcccc
    50401 ggacatgacc acaggcagag aaaacatccc tgcgccaata atggtgccgc cgatgatcac
    50461 cacgccgcca agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc
    50521 tctccagtga aaaatagtgc gactgcgttg ttatgcactg cactgtacca gtacacgagt
    50581 acaaaagaca gaaaaaaagc cccgatggta aaaatcgggg ctgtatatat tattttacag
    50641 attgtgttcg ctgtttagcg aagattacgc atcaccaccg aaacgacgac gaccggtaga
    50701 gtcatcacga cgcggagcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc
    50761 acgaccacct tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gttcgccacc
    50821 gaaaccacga ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa
    50881 ctgcatgttc atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc
    50941 cggcatacct ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccaat
    51001 gtaacggctg ctgatgtcac cttcgttagc aatcgcgcca acgatatgac gaacttcaac
    51061 accatcatcg cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc
    51121 acgacgcgga cgatcttcac ggtcaccacg cgggccacgg tcgttacgat cgcgcggacc
    51181 acgatcatca cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat
    51241 cagagtacgt tcaccctgtg ccattttcag cagtgcggcg gccagagttt cgagatccag
    51301 ctcttcacct tctgccgtcg gctgaatttt gctcagcagt gcacggtatt gatccagatc
    51361 gctgctttcc agctgctgct gtactttagc ggcgaatttt tccagacgac gtttgcccag
    51421 cagttctgcg ttcggcagtt caacttccgg aatggtcagc ttcatggtgc gttcaatgtt
    51481 acgcagcaga cgacgctcac ggttctcaac gaacagcagc gcacggccag cacggcccgc
    51541 acgaccggta cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt
    51601 aaccaccagg ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag
    51661 gatgtccaga cgaccgtctt tcaggcgttc cagagtctgt tcacgcagcg cctggttcat
    51721 gtcaccgttc agcgcggcgc tgttgtagcc gttacgctca agagcttcag ccacttccag
    51781 agtcgcattt ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa
    51841 acgtaccagt gcttcgtttt tacgcatacc ccagacagtc cagtagctct ggctgatgtc
    51901 aggacgggta gtcacgctgg actgaatgcg cacttcctgc ggctctttca taaagcggcg
    51961 ggtaatgcga cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc
    52021 cgggatctgc gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc
    52081 agcttcatcc agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg
    52141 gtccagcaga cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa
    52201 ttgcacgtca taacgctggc cgccgtacag ggcaaccacg tttacgccgc gcatgtgttt
    52261 agagaaatcc gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac
    52321 cagaatctgt ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc
    52381 tgcagttttt ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg
    52441 tggaatacac tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc
    52501 aaggatagga gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta
    52561 cgtgtgcctc aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa
    52621 ttttcattga aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc
    52681 acccgttaag gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc
    52741 tggtccgatt ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg
    52801 tatcggtgct caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg
    52861 gcgctgtcca aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg
    52921 agatgctcag cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt
    52981 tgttcgctaa tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc
    53041 gatttttcga agtgctgttt caacacttct ttagcctgct tctcatcgag cttctgctcg
    53101 gcgagataaa gccgcagact acggaaagga tcattgggat cgtcttgata aaacgccagc
    53161 agatcatctt gcgctaactt gtcacgaccg ccgtaatata atgcgatccc gcgattcaag
    53221 tgcgcgtagt tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca
    53281 tcaaaattgc ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc
    53341 ggtcggattg ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta
    53401 tcatacaaca ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga
    53461 ctggcaagga tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat
    53521 ggtaccgcga ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt
    53581 gctgtcgcaa cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg
    53641 ttgaatgaac gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc
    53701 gccgcagcgg agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt
    53761 gctgcagcag gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg
    53821 cgatcaactt ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact
    53881 ttctcaacgc gtttgtcagc gatctgagag atgtgtacca gaccttcttt accgccgccg
    53941 atggcaacaa atgcgccaaa gtcaacgata cgggtcactt taccattgta gacgcggccc
    54001 acttcgattt ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg
    54061 tcggtcgctg cgatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct
    54121 tcggtcaggg cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg
    54181 ttgatcttga tggtatggat acgcggagcg aactcagaga tatcgccacg tggcgcgttg
    54241 attgcctgtt ccattacgcc caggatatgc agacgcgcac ctttagcctg attcagcgca
    54301 acctgcatga tctctttggt gataccttca attttgatat ccatctgcag tgcagagata
    54361 ccgtcgcggg aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa
    54421 atgtcagaca gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca
    54481 acagcagctt tgattggcac acctgcgtcc atcagcgcca gggacgcgcc gcacacggaa
    54541 gccatagaag aagaaccgtt ggattcggtg atttcagaca ccacacgtac ggtgtacggg
    54601 aatttgtcca tatccggcat gactgccagc acaccgcgct tcgccagacg accgtgacca
    54661 atttcacgac gtttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag
    54721 ttgtagtgga acaggaaggt gtcagtacgt tcgcccatca gttcatcaag aacctgcgcg
    54781 tcacgtgcag tacccagcgt tgcggtaacc agcgcctggg tttcaccacg ggtgaacagc
    54841 gcagaaccgt gagtacgcgg cagcacgcca gtacgcacat ccagaccacg aatcatatct
    54901 ttttcacgac cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc
    54961 tcgatagcgt gcagaatttc acccagttcg ttttcgtcca gggtttcatc ttcagcaagc
    55021 agcgtcgcga tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg
    55081 gtgatgcggt aagcatcgct cagacgagct tcagccagtg cagcaacgcg cgcgttcagc
    55141 gcttcgttta ccggctccgg ctgccagtcc caacgcggtt taccggcttc tttcaccagt
    55201 tcattgatgt tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc
    55261 atctggtctt cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta
    55321 ccggcaacaa ccagatccag tttgctctct ttcagctcgt cctgagtcgg gttcagtacg
    55381 tactggtcat tgatgtaacc tacgcgggca gcaccaatcg gaccattgaa cggaatacca
    55441 gacagagaca gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacctgcggg
    55501 ttaacagaaa ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc
    55561 gggcgaatcg ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct
    55621 tcacgacgga agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta
    55681 acggtcagtg ggaagaagtc ctgacctggt ttggcttttt tctgtccaac aacggtaacg
    55741 aataccgcgg tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg
    55801 ccggtttcca gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc
    55861 aatgtaatat cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat
    55921 cttctgcgca tcctcgcgac taatgacaac cctaacccag cccaatgtgg gtaaagcctc
    55981 tcattagccg cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc
    56041 cagtgaattg ctgccgtcag cttgaaaaaa ggggcctctc aggccccctt ttctgaaact
    56101 cgcaagaatt agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct
    56161 ttacgtttca ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga
    56221 cggctgtggt gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca
    56281 gtcagcagtg ctacctgaac ttcggtagaa ccggtgtcgt ttgcgtcacg accaaactca
    56341 gaaacgattt tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag
    56401 aatgaaagga cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa
    56461 caatttacgc gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg
    56521 ttaacattac gccgggtatt caaccaccag gcgacgaggc gcgacgcggc cttcatcgtc
    56581 aatttcgccc ataccgataa acttgccgtt ctcaccttct gtaacgcgaa ccagtccttc
    56641 cagcggcgca ccggatgtac gaaccgggtt accatttttg aagtaaacag aagacgttaa
    56701 cggaagattc accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc
    56761 aagtaactcc gcggctggaa tatcctgctg ttcggcttgt tcaaccagtt cacgcagatg
    56821 ttccagggtc accatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat
    56881 aacatgcgcg ccacaatcga gtttttcacc caggtcatca atgatggtgc ggatataggt
    56941 ccctttagag caatgaactt ccagctccag ttcattgcct tcatggcgaa taaacagcaa
    57001 ttcataaacg gtaatcgggc gtgcttcacg cggaacttca atgccctgac gcgcatattc
    57061 gtacagtttt ttgccctggt atttcagtgc tgaatacatc gatgggatct gttcgatatc
    57121 gccacggaaa gtatccagtg ccgccgccag ttgctctgtg tcaaaggtta ccggacgttc
    57181 ttcaacgatt tgtccatcgg cgtcagacgt atcggtacgc tgtccaagac gcgcaatcac
    57241 ccgatagcgt ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca
    57301 aattggcaac atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc
    57361 gttataaatc cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc
    57421 cagcaacaaa acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc
    57481 ttgctgtcgt ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg
    57541 tttgacatgc gcatcccttc aaccagcgag ttgtcgtaga agaaggtcag ttccggcacg
    57601 atacgcaggc gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcctgcaac
    57661 gctttgatgc ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt tacatatact
    57721 ttggcatacg ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccaga
    57781 cgaggatctt taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct
    57841 acgcgctgcg ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggcc
    57901 atagcccctt ttaaaattaa tctcaggtgg aaagggc
//
LOCUS       DAPOZO010000021        57414 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPOZO010000021 DAPOZO010000000
VERSION     DAPOZO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.021"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..787)
                     /locus_tag="ROI21_001377"
     CDS             complement(<1..787)
                     /locus_tag="ROI21_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDX5110322.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVASGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSFQLPDDEIGFIALHILN
                     NYENSSDYESVRIIELSQKITEIIEGIYNRKVDRSSFNYSRFMMHLKYFSNRVLCNEK
                     IKQKDIGDIYEQFLEKDILLQRAIHEIERYLYATFKYELILEEK"
     gene            complement(1045..2298)
                     /gene="amiC"
                     /locus_tag="ROI21_001378"
     CDS             complement(1045..2298)
                     /gene="amiC"
                     /locus_tag="ROI21_001378"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDX5110323.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2530..3861
                     /gene="argA"
                     /locus_tag="ROI21_001379"
     CDS             2530..3861
                     /gene="argA"
                     /locus_tag="ROI21_001379"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDX5110324.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVIYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3923..5749)
                     /gene="recD"
                     /locus_tag="ROI21_001380"
     CDS             complement(3923..5749)
                     /gene="recD"
                     /locus_tag="ROI21_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDX5110325.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFSD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5749..9291)
                     /gene="recB"
                     /locus_tag="ROI21_001381"
     CDS             complement(5749..9291)
                     /gene="recB"
                     /locus_tag="ROI21_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDX5110326.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGKVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARKIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVLTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(9284..12172)
                     /gene="ptrA"
                     /locus_tag="ROI21_001382"
     CDS             complement(9284..12172)
                     /gene="ptrA"
                     /locus_tag="ROI21_001382"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDX5110327.1"
                     /translation="MPSSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFQEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKSADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRTMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12348..15716)
                     /gene="recC"
                     /locus_tag="ROI21_001383"
     CDS             complement(12348..15716)
                     /gene="recC"
                     /locus_tag="ROI21_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDX5110328.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLEGSQELDAFVDVTPDNL
                     LHNIQSDILELENCAVAGVNIEEFSRSDNKRLLDPLDNSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15729..16052)
                     /locus_tag="ROI21_001384"
     CDS             complement(15729..16052)
                     /locus_tag="ROI21_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDX5110329.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(16037..16444)
                     /locus_tag="ROI21_001385"
     CDS             complement(16037..16444)
                     /locus_tag="ROI21_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDX5110330.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTQPAVQCLLYAATGARVCLRLLVDNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPCGWSDFCPLKERALCQLP"
     gene            complement(16441..17004)
                     /locus_tag="ROI21_001386"
     CDS             complement(16441..17004)
                     /locus_tag="ROI21_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDX5110331.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRDSLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEDKGWDKVTNPDAIIIDTFQVTRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGFNL"
     gene            complement(16995..17465)
                     /locus_tag="ROI21_001387"
     CDS             complement(16995..17465)
                     /locus_tag="ROI21_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDX5110332.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17651..18445)
                     /gene="thyA"
                     /locus_tag="ROI21_001388"
     CDS             complement(17651..18445)
                     /gene="thyA"
                     /locus_tag="ROI21_001388"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDX5110333.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18452..19327)
                     /gene="lgt"
                     /locus_tag="ROI21_001389"
     CDS             complement(18452..19327)
                     /gene="lgt"
                     /locus_tag="ROI21_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDX5110334.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19478..21724)
                     /gene="ptsP"
                     /locus_tag="ROI21_001390"
     CDS             complement(19478..21724)
                     /gene="ptsP"
                     /locus_tag="ROI21_001390"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDX5110335.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21737..22267)
                     /gene="rppH"
                     /locus_tag="ROI21_001391"
     CDS             complement(21737..22267)
                     /gene="rppH"
                     /locus_tag="ROI21_001391"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDX5110336.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22952..23191
                     /locus_tag="ROI21_001392"
                     /pseudo
     CDS             22952..23191
                     /locus_tag="ROI21_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="MutH; Sequence-specific endonuclease that cleaves
                     unmethylated GATC sequences during DNA repair; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutH"
     gene            23191..23610
                     /locus_tag="ROI21_001393"
                     /pseudo
     CDS             23191..23610
                     /locus_tag="ROI21_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
     gene            23732..24625
                     /gene="nanA"
                     /locus_tag="ROI21_001394"
     CDS             23732..24625
                     /gene="nanA"
                     /locus_tag="ROI21_001394"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDX5110337.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            24734..26224
                     /gene="nanT"
                     /locus_tag="ROI21_001395"
     CDS             24734..26224
                     /gene="nanT"
                     /locus_tag="ROI21_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDX5110338.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAVIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            26272..26961
                     /locus_tag="ROI21_001396"
     CDS             26272..26961
                     /locus_tag="ROI21_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDX5110339.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            26958..27833
                     /gene="nanK"
                     /locus_tag="ROI21_001397"
     CDS             26958..27833
                     /gene="nanK"
                     /locus_tag="ROI21_001397"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDX5110340.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDITEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTHAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            27830..28294
                     /locus_tag="ROI21_001398"
     CDS             27830..28294
                     /locus_tag="ROI21_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDX5110341.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPDEIKKVVVKVKADLMA"
     gene            complement(28354..29466)
                     /locus_tag="ROI21_001399"
     CDS             complement(28354..29466)
                     /locus_tag="ROI21_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDX5110342.1"
                     /translation="MESLSEGTTAGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSTRNLWQFKNFYICFQRIEI
                     VQTLSAQFTPTILQTVSGESCHLAQLAKSFPLPWSTYVRLLSVKKADARSFYEKETLR
                     CGWSARQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLEFLELK
                     DEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLL
                     IVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYALAGLP
                     NTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(29650..31068)
                     /gene="gltD"
                     /locus_tag="ROI21_001400"
     CDS             complement(29650..31068)
                     /gene="gltD"
                     /locus_tag="ROI21_001400"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDX5110343.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDISGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(31081..35541)
                     /gene="gltB"
                     /locus_tag="ROI21_001401"
     CDS             complement(31081..35541)
                     /gene="gltB"
                     /locus_tag="ROI21_001401"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDX5110344.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            36216..37145
                     /locus_tag="ROI21_001402"
     CDS             36216..37145
                     /locus_tag="ROI21_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDX5110345.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTANDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            37241..39577
                     /gene="arcB"
                     /locus_tag="ROI21_001403"
     CDS             37241..39577
                     /gene="arcB"
                     /locus_tag="ROI21_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDX5110346.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPVLTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            39807..40460
                     /gene="elbB"
                     /locus_tag="ROI21_001404"
     CDS             39807..40460
                     /gene="elbB"
                     /locus_tag="ROI21_001404"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDX5110347.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            40457..41185
                     /gene="mtgA"
                     /locus_tag="ROI21_001405"
     CDS             40457..41185
                     /gene="mtgA"
                     /locus_tag="ROI21_001405"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDX5110348.1"
                     /translation="MSKSRLTVFSFIRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVNMDQISPWMGLAVIAAEDQKFPEHWGFDVTSIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(41182..41814)
                     /gene="yrbL"
                     /locus_tag="ROI21_001406"
     CDS             complement(41182..41814)
                     /gene="yrbL"
                     /locus_tag="ROI21_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDX5110349.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAAQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALTIALQKDLQPRESKTLTLTSREA"
     gene            complement(42027..42299)
                     /gene="npr"
                     /locus_tag="ROI21_001407"
     CDS             complement(42027..42299)
                     /gene="npr"
                     /locus_tag="ROI21_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDX5110350.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(42296..43150)
                     /gene="rapZ"
                     /locus_tag="ROI21_001408"
     CDS             complement(42296..43150)
                     /gene="rapZ"
                     /locus_tag="ROI21_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDX5110351.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(43196..43687)
                     /gene="ptsN"
                     /locus_tag="ROI21_001409"
     CDS             complement(43196..43687)
                     /gene="ptsN"
                     /locus_tag="ROI21_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDX5110352.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(43805..44092)
                     /gene="hpf"
                     /locus_tag="ROI21_001410"
     CDS             complement(43805..44092)
                     /gene="hpf"
                     /locus_tag="ROI21_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDX5110353.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(44115..45548)
                     /gene="rpoN"
                     /locus_tag="ROI21_001411"
     CDS             complement(44115..45548)
                     /gene="rpoN"
                     /locus_tag="ROI21_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDX5110354.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(45596..46321)
                     /gene="lptB"
                     /locus_tag="ROI21_001412"
     CDS             complement(45596..46321)
                     /gene="lptB"
                     /locus_tag="ROI21_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDX5110355.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(46328..46885)
                     /gene="lptA"
                     /locus_tag="ROI21_001413"
     CDS             complement(46328..46885)
                     /gene="lptA"
                     /locus_tag="ROI21_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDX5110356.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(46854..47429)
                     /gene="lptC"
                     /locus_tag="ROI21_001414"
     CDS             complement(46854..47429)
                     /gene="lptC"
                     /locus_tag="ROI21_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDX5110357.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(47426..47992)
                     /gene="kdsC"
                     /locus_tag="ROI21_001415"
     CDS             complement(47426..47992)
                     /gene="kdsC"
                     /locus_tag="ROI21_001415"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDX5110358.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLLPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(48013..48999)
                     /gene="kdsD"
                     /locus_tag="ROI21_001416"
     CDS             complement(48013..48999)
                     /gene="kdsD"
                     /locus_tag="ROI21_001416"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDX5110359.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(49013..49990)
                     /locus_tag="ROI21_001417"
     CDS             complement(49013..49990)
                     /locus_tag="ROI21_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDX5110360.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQSGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50200..51009
                     /gene="mlaF"
                     /locus_tag="ROI21_001418"
     CDS             50200..51009
                     /gene="mlaF"
                     /locus_tag="ROI21_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDX5110361.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            51017..51799
                     /gene="mlaE"
                     /locus_tag="ROI21_001419"
     CDS             51017..51799
                     /gene="mlaE"
                     /locus_tag="ROI21_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDX5110362.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            51804..52355
                     /gene="mlaD"
                     /locus_tag="ROI21_001420"
     CDS             51804..52355
                     /gene="mlaD"
                     /locus_tag="ROI21_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDX5110363.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            52374..53009
                     /gene="mlaC"
                     /locus_tag="ROI21_001421"
     CDS             52374..53009
                     /gene="mlaC"
                     /locus_tag="ROI21_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDX5110364.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            53009..53302
                     /gene="mlaB"
                     /locus_tag="ROI21_001422"
     CDS             53009..53302
                     /gene="mlaB"
                     /locus_tag="ROI21_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDX5110365.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            53462..53716
                     /gene="ibaG"
                     /locus_tag="ROI21_001423"
     CDS             53462..53716
                     /gene="ibaG"
                     /locus_tag="ROI21_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDX5110366.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            53771..55030
                     /gene="murA"
                     /locus_tag="ROI21_001424"
     CDS             53771..55030
                     /gene="murA"
                     /locus_tag="ROI21_001424"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDX5110367.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(55078..55356)
                     /gene="sfsB"
                     /locus_tag="ROI21_001425"
     CDS             complement(55078..55356)
                     /gene="sfsB"
                     /locus_tag="ROI21_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDX5110368.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(55584..56555)
                     /gene="ispB"
                     /locus_tag="ROI21_001426"
     CDS             complement(55584..56555)
                     /gene="ispB"
                     /locus_tag="ROI21_001426"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDX5110369.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            56814..57125
                     /gene="rplU"
                     /locus_tag="ROI21_001427"
     CDS             56814..57125
                     /gene="rplU"
                     /locus_tag="ROI21_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDX5110370.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            57146..57403
                     /gene="rpmA"
                     /locus_tag="ROI21_001428"
     CDS             57146..57403
                     /gene="rpmA"
                     /locus_tag="ROI21_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDX5110371.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
BASE COUNT        13555 a        14993 c        14899 g        13967 t
ORIGIN      
        1 gtttttcttc taaaattaat tcatatttaa aagtggcata cagatacctt tcaatttcat
       61 gaattgcgcg ctgtaacagg atgtctttct caagaaactg ctcatagatg tcaccaatat
      121 ctttctgttt tatcttttca ttacataaaa ctcgattaga aaaatactta aggtgcatca
      181 taaatcttga atagttaaat gaacttctgt ccacctttcg gttataaata ccctcgataa
      241 tctccgtgat tttttgagat aattcaataa tccgtaccga ttcataatca gatgaatttt
      301 catagttatt taaaatatgc aatgcaataa aaccaatctc atcatccgga agttgaaagc
      361 taaattgctc attgatgatg gttaatgcat cccggcttat tgcatattct gctttgtaca
      421 acgctttgat ttcatcaagg aaaacattat taatttgtat acctgaagcg actcgggata
      481 ttgcattcga aatgtgatcc gcaagaatca atagtagcga tggattgaga tttttttcga
      541 gatttttctc cgcatattgc actatttttt cggcaacaaa cacatactca atatctacat
      601 gttcaatcaa tttatagagt ttgttttttt gttcatttct gacataaaaa atccggttag
      661 ccggatgctc agggaccgac attccatatt ttttgttgta accgacgcca ggtccagaaa
      721 tgataacctc ctgtccattc atcgatgcct gtacacaatt attgttcatg actttttcga
      781 tgatcattcc atgactccaa aaaaaaaggc aaggcacgtc agaagtaata attacttcaa
      841 acattgcctt gcctgattta acagttacaa gatgattaac taatgtgtat acattacacg
      901 gaattgtgtt taattaaaga gatggtcggc ttatttttca agccgacatt caaatattcg
      961 acatcagtca caaaaccagc atctaatatt catgaaaatg ttatctattc aacgaaatat
     1021 aaattcattg aataaataaa aacatcatcc ccttctcgcc agcgtcgccc catcggcaaa
     1081 atacgcttta atccccgcaa gaatagactc cgcaacttcc tgctggaagg tcgccgtttt
     1141 cagtttacgc tcttcctcaa cgttactgat aaacgccgtt tcgaccagaa tggagggaat
     1201 atctggtgcc tttagtacgg caaacccggc ctgttcaacc tgatttttat gcagcttgtt
     1261 gattttaccg agcttattca gcaccgcttt accaaacttc aggctgtcgg caatggtcag
     1321 cgactgcacc atatcgaaca tggtgtggtc gacatagcgg tcaccgcttt tgctcacgcc
     1381 accaatcaag tccgaggcgt tctgggtttg tgccagatat tttgccgcag tactggttgc
     1441 acctttggtt gagagcgcaa acacagagga accgctcggc tgacggctgg taaaggcgtc
     1501 ggcatggata gagacaaaca ggtcagcacg ctgtttctgg gcttttgcta cgcgcacttg
     1561 caacggaatg aagatgtctt cattgcgcgt catgtacacc ttcatattgc cctctttctc
     1621 gatcagagag cgcagacggc gagctatttg caataccacg tctttttcgc gcgttttgta
     1681 tttccccacc gcaccggagt cttcgccacc gtggccaggg tcaagcataa tgacaatcgg
     1741 acgatcgcgc cctgctttac ccggttgtgg accactttgt gctggcggca cctgcttttc
     1801 gaggtcgcct ttgttgtaat cctccagcag cgccagcagc gggtcctgca tatcctgtgc
     1861 attggccgga tagaggtcca tcaccagacg ctctttaaac ccagcgaccg gcgcaagggc
     1921 aaacagctgc ggttttacgt tttgctttaa ttcaaaaacc atgcgtacgg tctgcgggtc
     1981 aaattgcccg acgcgcgccg acttgatgaa cgggtcgtct gcacgaattt gtgccgccat
     2041 ccccttgagt accgagttca ggtttacatc ttcgatatcc accaccacgc gttcagggtt
     2101 actcaacgcg aactgcttat atttcagctg acgatttgat tctaccgtca cgcgggtgta
     2161 gctggacgca ggccagacgc gcaccgccac gacctggctg accgcagcca gactgacctg
     2221 acttacgctc aatagccaca tggcacccgc gccttgcagt aaacgacgac ggctgattgc
     2281 agtgttggat cctgacatgc ctctcccgag caaaagaaat ctaagctgtg taacaagtaa
     2341 acgactaatt tgaccggaaa ctttagcgaa agacgcataa tctgtcatct aataaacggt
     2401 aaacattctt tttatattca cggcattact gataaaaaag tcgctctcgc ataaaattta
     2461 cacttgcacc ctgcgaaaaa acagaataaa aatacactaa tttcgaataa tcatgcaaag
     2521 aggtgtgccg tggtaaagga acgtaaaacc gagctggtcg agggattccg ccattcggtt
     2581 ccctatatca atacccaccg gggaaaaacg tttgtcatca tgctcggcgg tgaagccatt
     2641 gagcatgaga atttctccag tatcgttaat gatatcgggt tgctgcacag cctcggcatc
     2701 cgtctggtgg tgatctatgg cgcacgtccg cagatcgacg caaatctggc tgcgcatcac
     2761 cacgaaccgc tgtatcacaa gaatatacgt gtgaccgacg ccaaaacact ggaactggtg
     2821 aagcaggctg cgggaacatt gcaactggat attactgctc gcctgtcgat gagtctcaat
     2881 aacacgccgc tacagggcgc gcatatcaac gtcgtcagtg gcaattttat tatcgcccag
     2941 ccgctgggcg tcgatgacgg cgtggattac tgccatagcg ggcgtatccg gcgaattgat
     3001 gaagacgcga tccatcgtca actggacagc ggtgcaatag tgctaatggg gccggtcgct
     3061 gtttcagtca ctggcgagag ctttaatctg acctcggaag agattgccac tcaactagcc
     3121 atcaaactga aagctgaaaa gatgattggt ttttgctctt cccagggcgt tactaatgac
     3181 gacggtgata ttgtctccga acttttccct aacgaagcgc aagcgcgggt agaagcccag
     3241 gaagagaaag gcgattacaa ctccggtacg gtgcgctttt tgcgtggcgc agtgaaagcc
     3301 tgccgcagcg gcgtgcgtcg ctgtcattta atcagttatc aggaagatgg cgcgctgttg
     3361 caagagttgt tctcacgcga cggtatcggt acgcagattg tgatggaaag cgccgagcag
     3421 attcgtcgcg caacaatcaa cgatattggc ggtattctgg agttgattcg cccgctggag
     3481 cagcaaggta ttctggtacg ccgttctcgc gagcagctgg agatggaaat cgataaattc
     3541 accattattc agcgcgataa cacgactatt gcctgcgccg cgctctatcc gttcccggaa
     3601 gagaagattg gggaaatggc ctgtgtggca gttcacccgg attaccgcag ttcatcacgg
     3661 ggcgaggttc tgctggaacg cattgccgct caggcgaagc agagcggctt aagcaaatta
     3721 tttgtgctga ccacgcgcag tattcactgg ttccaggaac gtggatttac cccagtggat
     3781 attgatttac tgcccgagag caaaaagcag ttgtacaact accagcgtaa atccaaagtg
     3841 ttgatggcgg atttggggta agcgatgaaa atcgtcggat gcgacatgcg taacactcgt
     3901 gcgtcgcatc cggcaattac gtttattccc gtgaactaaa caacgccgcc agaccactgc
     3961 gccgctcagt acgagtggcg attgccgcac ttaatatgcg ctcatcggca tacagcgaca
     4021 gacggcgacg cgcgcgggtc accgcggtgt aaaccagctc tcgtgttact accggcgtgc
     4081 gttggctcgg taaaatcaac gccgcatggt cgaactccga tccctgcgat ttatgtaccg
     4141 tcatcgccca cgtagtttcg tgctctggca ggcgactcgg ttgcacagac ttaatattgc
     4201 cgtccggcat cgcaaaccaa acgcgcgtcc cctgtccgcg atccagagcg ataccgatat
     4261 cgccattaaa caacccaagc gcgctgtcat tacgggcaat catcaccggt cggccttcgt
     4321 accaacgaga gtgcggatta cgatgaattt tgcgcttctg ttgcataaac tgctcaattc
     4381 gctcattcag tccagccacg ccaaacggcc cttcccgcag ggcgcacaaa agctggtact
     4441 cattgaacgc ctgaatgatt aaatccggct cggcacgcgc ttgcagcaga tccagataac
     4501 gtccgtaacc cgcaagagct tcctcaagca tcgcaatata atcttcgccg ctctgtaaaa
     4561 gccgtttttc gatatcagaa aaatcctgct gaaaaacggt tttcactgcc gttttatcac
     4621 cacggttgat cgccgcagct aactgaccaa tgccagaatc actgccgaaa cgatagcttt
     4681 tttgcagcag gcagagactg tcgcgcaaag atgccgcttc tgtgccagtt cctgccggaa
     4741 cgtgagttcc cgtcaggcgg ctcaactgcc cggcacgctc ggcggtaaag cccgcgttgg
     4801 cataagcgca gatatcgccc agcacagccc cagcctcaac cgaggccagt tgatcacgat
     4861 cgccgagaaa gatcactcgc gcatgatcgg gcaaggcgtc gatcagtctc gacatcatag
     4921 gcagatcgat cattgacgct tcatctacca ccagcacatc aagatgcagc gggttaccgg
     4981 catgatgacg taaacgctgg ctacccggct gcgcacccag caatcggtgc aaagtactgg
     5041 catcttccgg aatgcgtttc ttttgttcat cggtcagcgg taactgtcgc aaagccttgc
     5101 cgagagattc ggttaagcgc gcggcagctt tacccgttgg tgcagccaga cggatacggc
     5161 agcgttctcc gtcggccatt tgaattaagg ctgccagcaa cttcgctacg gtggtcgttt
     5221 taccggtgcc aggaccgccg gaaatcaccg aaatccgccg cgtcagcgcc actgccgccg
     5281 caactttttg ccagttaatt tcatcgctta ctggaaaaag tttgtccagg gtttgcgcca
     5341 gtagagcttc atcaacctca atggcatgat tcacttcgtt gaaaaagcgt gccactgtgc
     5401 gctcgttaca ccacatgcga ttcaaataaa gacgatcgcc acagaggatc atcggcgttg
     5461 gttcatctcc ccggcttacc gcttgtgaag ccagcaagca ttcttcccaa ttttgtaact
     5521 caccgatttc actgacacag gtcgccaaca gcggatgcga cgcctcgtta ttttccagtc
     5581 gtgagagcgg taaacagaca tgcccttccc cggcgtcgtg gcttaacagg gctgccgcga
     5641 gggtgacggc aggatgttca tctcccgcca cggtcagggc aaactgcacg tccagcgggc
     5701 gtagctgttt gtgctccaca gcttccagta attgcttttg caatttcatt acgcctcctc
     5761 cagggtcata ccggcaaaca tctcatccat caagtcaatc aacccggcgt tgggtcgggt
     5821 tgtgtaaatc ccttgttgcg gatgttcttt atcaacgcca cgcaggaaca gatagataac
     5881 gccgccaaag tgacgatcat agtcgtaatc agcaatgcga tggcgcaaat aacgatgcaa
     5941 cgccagggta taaagctgat attgcagatc atagcggtgt gcctgcatcg ccgctgccat
     6001 agcctgttgg gtgtaagccg aactgtcttc acccagccag ttggatttat agtcgagcag
     6061 gtaataacgc ccttcatgac gaaataccag gtcgataaag ccttttaaca tgccgcgtac
     6121 ctgcatgaac tccagcggcg ggcagccagc ggatagcgga tcaaactggc gaattagtgc
     6181 atcaagctgg ctggcgatga gcggttcact aatcggcagg taaaactcca tctccacctg
     6241 tttatcgcgg gcggaaagct gactcaggct aacacccgtt tcattgagag gtgcctggag
     6301 aacagccgtg atccactcgg tcaacactgg ttcccactgc gattcaaagc cgccgagttc
     6361 cagtttttcc tgtacccagt tcgggtcaac cggctgggta aaatcgaggt cttcaaacaa
     6421 actgtgcaag aatgtccctg gtgacgcacc gcgcgggaac tgatgcggtg ttaacgtcgg
     6481 ttcttcaacg acgctgacca cgcctgcggc atcaacatcc agccgtggca tcaaatcctg
     6541 agcgataccg tgaccacgct gctgcaaacc ggagtagctg gtgacacgcc agttatcgcc
     6601 gggcaatcgt tgtagcgtcc tcgcattcag ttctgcagtt aaaacatcat taacctgcca
     6661 gggctggtta tcaccagttt gtgccgtttg ccaggcaata tcatcatcgc ataaagcttc
     6721 aatacaggtg cgaagccctg ccgcatcttg cggttcccct ttttgtagca aacgcccgag
     6781 cgcactttgg tggacgtcgg tgtcaccttt tttatcgcca cgacggcgca ccagcggtgc
     6841 aacgccgaga ctgcaatgcc aaaccgaacg cgtcagcgcc acgtaaagca aacgcagatc
     6901 ttccgccaga cgttcggcct ccgcgagatc gacactttct ggcgcagcgt taagatccag
     6961 aacagcctca aacgagtggc gatcgtgata gaacgcctga tcctggacgc ggaaattggt
     7021 aataaatggc aaccagacca gcggatattc cagccctttt gatttatgga tcgtgacaat
     7081 ctgcaccaga tgtttatcac tttcgaggcg catttgttgg ctggaggcat tactgtctgg
     7141 ctcgaggata tgttgcgata accagcgtac cagcgcatgt tcactttcca gctgcgttcc
     7201 ggcttcttgt agcagttcgc tgatatgcaa gatatcggta agacgccgct caccgcctgc
     7261 cgttgccagc aagttttctg caatcttacg cgccgacatc agcgcccgca gcatcggcat
     7321 aacgccacgt ttgcgccaga tttgccgata accatcgaac tcttcgacta ccgcatccca
     7381 cgcatgttcg tcattgttca gcgtttcgat atccagcgcg ttcagcccca tcattgacgt
     7441 tgccagcgca ctacgcaggg tgttctcacg ttcgggcgtc atcaccgcct gcaacaacca
     7501 aagcatttcc tgcgcttcca gagtttcaaa aacactgtcg cggttcgaaa ggtaaacgga
     7561 agggatttcc agcaacgtta aggcgtcgcg cacctgggcg gcctcctggc ggctgcgcac
     7621 cagcacactg atgtccgaag cacgcaccgg acgcgcgtcg tcgccgttca tcagcaacgc
     7681 ttcgccccgc tgtccggctt gtagccagtc gcggatttgc gcagcacata cctgcgccat
     7741 agtactttga tagtcgccaa cgccgcagct ttcgccttcc atcagccaca ttttcatcgc
     7801 aggctgcgtt tcacctttaa atacaaaacg taacgcctga tttttcccgg ctgatttcac
     7861 tggaataaac ggtatttcgc gaaacatgaa cgcgtcatca gtctggctga aaagcttatt
     7921 cacgctgttc accattcctg gtgcggaacg ccagttggtg tctaaagtgt agtgggcgtg
     7981 aacttcgcta cgcgccttca tataagtgaa gatatccgca ccccggaatg catatatggc
     8041 ctgtttcggg tcgccaatca gcaacaatgc ggtttccggc tgatggtgcc agatacggcg
     8101 aaaaattcgg tactgctggg ggtcggtatc ctgaaattca tcgatcattg ccaccgggaa
     8161 tcgcgtacgg atcgccgctg ccaacacctt tccgctttcg ctacgcagcg cggaatcgag
     8221 ccgacttaac atgtcatcaa aacccaattc gccacggcgg cgtttttcac gcgctactgt
     8281 ttcgcggatc tcagccaatg cgcgggttat caccagatca cggatcgaca atggttctgc
     8341 aagcagttga tcgatcgcct caaacagtgg atgtcgcggg gtttcccccc cggctttcgt
     8401 gcgatcttct aagaaacgct gagagaattt ttccagcgac tccggtaact gataactgtt
     8461 ggtctcttct tctgcccagg cgctgatctt ctcgatccat ttagcctgat tgctacggtt
     8521 aaacttgcgt cgatcaatac cagaagattc gatcagcgca tccagttcac ccactgcgtc
     8581 gcgccactgc tgttttacgg cgtcgatacg cgccacaatt tgcgcgtggc gggaagccag
     8641 cgtttcatca tcgggcggcg gtgctttgat aaccggtgct tcgccttgca gataacgatt
     8701 aatatcgcgc agcaacgcct gcggcccttt ccaggtttca aagaccacct gggcaatttc
     8761 acgcggcagc gggtagcagt ggcgacgcca gaaatcggcg caggcctggt agcgtagcag
     8821 agactcatct tcaatcagct gctgctcaaa cagcatgccg gattcaaagg cattcaggtt
     8881 gagcatgcgc tggcaaaaac cgtgaatagt aaagactgcc gcttcatcca tctgccgttc
     8941 ggctaacaac aaccactgcg cggcttgcgc tttatcgtcg atctcttcca gcaggcgttc
     9001 gtacagtgga ttgtcggtgg tttcacgcag acaggcgatg cgcaactcgt ggatattgct
     9061 acggatacga ccgcgcaatt ctgccgtggc agcctcggta aaggtgacca ccagcagttc
     9121 ttcaacggtc agcgggcggg gaaaggcggc ggaaccgcct agtccaagta acaggcgcaa
     9181 atagagcgcc gcaatcgtaa aggttttgcc tgtgccggca gaggcttcaa tcaggcgctc
     9241 gccctgtaag ggcaagcgca aaggatctag tgtctcagcg acatcactca ttcttttcac
     9301 tcatcaaggg cattgtttgc tgcaacgcgc tgacgttctc ccacactttc cagccttcag
     9361 gatgcacata ttcggctttc ccgttctggc tgccggaaat ctgcgacaga atagccatgc
     9421 cttgcggctc gaccaccgcc tgatggaaga aatcagcaag tttttgcggc gtcagcagtt
     9481 ttatctgggc cacgatttta tcacgcgaat cgaagcgcat attgccgcga tcgaaatctt
     9541 tacttaactt cgatgcttct tcgccgagcg tttgcggtgc ctgcagcatc tgggttatta
     9601 ccgcctgctg gatttgcgca aactcttctg gcttcatcgt ccgcaatttt gcctctgcgg
     9661 ttgggaaaaa cgccttgtaa cgctcccaca agaatgaagg ctgtttatca ttgctttgca
     9721 acaggaagcc catgccccac tgacgcccca cgctcattgg aaacgcaaac acggcatagc
     9781 ccaactgttc ttcggtacgc aactgattgt agaaccacgg ctgtacgatc tgccccaaca
     9841 gagagctata ggctgagctg gtgtattcat cgtagccagt cggtacaaat accgctgcca
     9901 gtgcggagtc ggtgctgtta ccggcttttt caaagatgac agattgtttt ttatcgacca
     9961 cgacatcttt gttacgacac cactccgaac catcagcgcc caactgtttt tgcacatcgc
    10021 gtgccagcgt tgttgcctgg gcttcggtca tgttgccaat aaccataaac tctggtcgag
    10081 cccctgattt taaggcgtcg cgataggcca gcacctcttt caaagtgatt gaaggcaaaa
    10141 ttttgcgccg ttcatctcgc gagaagtacg gcacttgcga gagcatctgc gcgggcataa
    10201 tcgcctgctc gaacgctttg cccttttctg cggaatccat catctggtta taccaggact
    10261 tcgcctgctc aagctgatct tccgtagcgg tatagctaaa gtacccctcg agcaatgcct
    10321 ggaacagctg cggcaggcgc tgggtgtaac cattggcatt aaccataagg ccgttgttag
    10381 cgttggtgga aaaacttatg ccaccaaccg acgcctggtt gcttaactga tcaagcgcca
    10441 gccctgcgag ataatcattg agcgcaaaca tcacctgatt gcgggcgctg tccatggctt
    10501 tcggattacg caaaatcagg ctgacatcag ctttgggttc gctggaaaaa taacggcttg
    10561 gcgcatacac cacacgcaga ttcgactcat caacaatcag ctctggatgg tcatatttct
    10621 tctctgactt aatcagcgag aaatcgtcag gaatataagg gttaagctct ggcaaagaga
    10681 gcgcaatgtc ggcggatttt ttctgccagt cggcgaaagt ttgtgcgctg attttatcga
    10741 cctgatacgg cgcatcgaca aagtaagccg ttttgttgtg cggctctttc gggctgatat
    10801 accagatacg cgcattctgc ggcgtcatca tcgccaggcg ttcttttact gctttagcgt
    10861 cgtaccgatc ggcaatattg actgcatcca gcgtatgctc aacaggaacg cgaatcatgg
    10921 tatctgccag ccattcgacg taatccatat cacgggtgat tgacggataa cgaaagtcga
    10981 tatccagcac attcgccagt tcatcgaagt attgtttatc aatccctttt tcgcgtaaca
    11041 gattgaggta gctaaaaatg gccgccacaa cctgatcgcg attcgccaga cctttatcgg
    11101 ttaaagacgc agagatcgct aatacgccgc tgttgccgtt gacgatagga tcggaattgg
    11161 cgctaatgcc ctcaactaat ccctgctttt gcagccagtc agaaagtgta cctgggctgc
    11221 gattaccgat cagataggta atcaattcat ccgttttact acggaacttc gctgaattgt
    11281 tatcgatgcg aaactcaacg cgcagaactt tacgcggcag cgccgggacg taatgaatga
    11341 taatgccctt ttgcgcgtcg gtgactaccg gcacggtgat ttccggtttt ttgctctctt
    11401 tgttcggcac gcgaccaaag gtgtccgccg ccatttttgc caactccggc agcggtttat
    11461 tgctgtaaat aaccgccttc atcagattgg cggaatagta cttctcctgg aaatctttca
    11521 gcgcctgctg taccggattt ccgggtttgt cgcttaaagt ttcgaggtta ccaccagaaa
    11581 actttgaacc ggggtgtgcc gggttaatgg tttctgcgct gacctgtgcc atgcgcatcc
    11641 cgtcacgcgt acgcgccatg gttaattcgg cattcactgc attacgttca cgttcggcgt
    11701 atttcttgtc gagcaagggt tctgcaatag cgtccgccag gcggtccacc gcaccgggca
    11761 aggcgtcgtt ctcaacttcc agatagaaag ccgtgcgata cggtgccgtg ctggcattgt
    11821 gactgccgcc gtgcattttg agatattcgg ccagactgtc agcctgcggg tactttttcg
    11881 accccatcag actcatatgt tcaaggtaat gtgccagccc ctggtacgcc tcgggatctt
    11941 ccagcgaccc aacgggcact accagcgccg agagcgattt aaccgcctgc ggatcagaaa
    12001 ccagcaagac caccataccg ttatccagac gtatagcctg atactggcgg ttatctttat
    12061 cacttttacg gatggtttcc tgaatcggct gccatcccgt ttctgcctga cttaagggtg
    12121 cccaaagggc aacaaacaac aataatgctt tgaaccaggt gctgctgggc attcacggac
    12181 ctcataatca actttatttt ctggccagat tcaacaggtt aacgcaaatc atctgccaga
    12241 atttaatctt gtgctgcacg agtcagccta tgtctatata tccatcagtc cgtgactggt
    12301 gcgcatcata aagcaagcag atatattgcg caatttttat acagcactca tgactgatta
    12361 aagcgaaaca gcggtaacag gaaacgttgc gactgttcaa cgatggcctc cattgtctct
    12421 ggtgttaatt gccgccagag ccgttgatac cagatatcat caccttcgcc acgcaccatc
    12481 atgttgcctt cgtaagcctg aaggaatttc gtacgggctt tttgtaacgt ggaatcgtca
    12541 tccagcatgg catcgttttg cgcgtcataa caggttttta gccacgcgcc gccactttca
    12601 ggtaacacca gcaatggcgc ggacattcct tcacgatacc cctcaatcag ttgtgagagg
    12661 taatgcaaag cctgttcggc tgcaagcggc ggaaaacgcc actcgccgtc tttgcgtaga
    12721 aaaaggcgac tttcaccatt accaccgctg gcacagtaga caaggtgttc cagccaaagt
    12781 tgcattccct gcgccacact taataaagag ggacgccagc gcaacaggcc atccggctgc
    12841 acctgcggca accagccagt tatctgcaca ccgttgcagg cgagatcaat ttccatactc
    12901 tgccccggct ggcgacaggc aataactctg tcggcaagct gctgcatctc ctggcactgt
    12961 gtttcccaga aaatttcacc aaaagcgcca tacggtaaat cccctgccgc ccggaagcgg
    13021 cggaacaagc gttcggcatc atcctgctca accagtgcat tcaataactg ctgattgatt
    13081 tgataacggc taagcccttc cagaataaat ggctcggtgt cggggatttc gctgtcttca
    13141 gtacggaagt tcacctgcaa acgcatctgg aagaacgccc gtaccggatg tgcccagaat
    13201 cgttgtagcg tttccagcgg cacggtttcc ggtaaggtaa acggcagcgg ctgaacaaat
    13261 tcagaatgtg ctttaccagc ctggctggcc gcaggtagcc attcacgagc atagctttgt
    13321 cgttcgcctg gctggtagtt ttgcggatca aacggcatcc gggtatggag gcaagtaaga
    13381 tgcgctttta cccttgcctc gctttcatca cagttgagcg cttcatcgcc cggtagataa
    13441 tggctttgcc cgatgtagtc gatcagttcc tgcaccagta ccgacgggaa acgttcactg
    13501 ttatcctgaa tagaacgccc gatatagctg atatagagtt tttgctgcgc ggaaattaac
    13561 gcttccagga acagatagcg gtcgtcatcg cgacgactac ggtcgccacg cttcggtttc
    13621 tggctcatca ggtcaaagcc caatggcgca agctggcgtg gataaacgcc gtcgttcatt
    13681 cccagcaggc aaaccacttt gaatgggatg gaacgcattg gcatcagagt acaaatgtta
    13741 accggcccgg cgagaaaacg ctggctgata cgttcctgat ccaggcgctg tgccagttca
    13801 tcacgcaata gtgacagcgg caccgcgtcg ccatactgcg cacctaaacc ttcggcgata
    13861 atcgcctgcc attgttgttc gatcagcgtc atcgccgctt cggtttccgc atccggcagg
    13921 aagaaggcgt tgagcatatc gcgacaaacc ggcaaccact cttccagcgg acgctcctgc
    13981 gccagcccgc gacgccagat gtttagctgc attagcagtg aagccagatg ccccaccagt
    14041 tctgcaatta agccgctcga ttcatcataa ggtagaaccg attgccactc gccctgcgca
    14101 ctctccatcg cgtagcccag caacatgcgc gtcaggccaa accgccaggt gtgttgaccg
    14161 gtagcgggga gttccagctc gcgaacgttg tcgtcatcta tcccccaacg aatgccggat
    14221 tcgttgaccc actggcgtaa ataacgcagc ccttcttcgg tgatgtcaaa ccgcgccgcc
    14281 agcaccggca catccagtaa tgccagaacg tcctccgaca caaagcggct gtctggcaac
    14341 gataacaggc tgataaacgc ttcaagtaca ggatgcgact gccgcgcccg acggtcggaa
    14401 atggcgtaag gtaggtaacg atccgcaggt gcgctaccaa acactgcctg aataaacgga
    14461 ctgtagctgt ctatgtcggc caccatcacg atgatgtcgc gcggagtcaa tgtcgggtct
    14521 tcctccagca ttgccagcag gcgatcgtgt aaaacttcaa cttcacgctg cgggctatgg
    14581 caaacgtgga aggtgatact gttatccagt ggatcaagca ggcgtttgtt atcgctacgg
    14641 gaaaactctt cgatattcac acccgcaaca gcgcagtttt caagttccag aatgtcagac
    14701 tgaatgttat gcagcaggtt atctggcgtg acatcgacaa aggcgtccag ctcctggctg
    14761 ccctccagat cagaaaggag ataaatgtag tcgcgcccaa gcttgcccca tgaggccagc
    14821 agcgggttgc cgacatcctg ttcaccatcg ctgttaaaga gctgtccggc attttcgctg
    14881 tcgcgaaata gcggtaattc gcgatcttca aaactgtgtc ggcgctggcg agtcagcagt
    14941 ttcgccagat aagcaggatc tttaatgtcg ccccagtaat aacggcaggg gttggtaaac
    15001 aggagatgga tttcaatatg tttacccagc gcctgtagcg cctggagata aacaggcggt
    15061 aacgcggaaa taccgcatat aaagacgcgc gaaggtagcc ccggcgggca ggtcgtcgcg
    15121 gactccagcg tttcgataaa gcgctgatag agattggcgc ggtgccagcg cggttgcccg
    15181 agttcatggg tatattccac caacgccttc cacaacggcg cttgccaggc ctgtgcttct
    15241 cccagccctt caaccaaatg tcctgtttcc cactgtgcca gccagtccgg acgatagacc
    15301 agatactggt caaacaggtc cgccgctttt gaagaaagct ggaacagttt tcgcttgtcg
    15361 ctatcgtcag tcagataatg ccgcaacagg gtaaagtctt cgcgctccaa cagttgcggc
    15421 agcagagtca tcagtttcca gctcatgctc tgtttgttaa aggcgctctc tttggggatt
    15481 tccggtaaca cccggacgaa catatcccag ataaagctcg ctggcagcgg aaaatcaatg
    15541 tttgccgcaa taccaaactt ttgcgacagg gtcatttgca gccactgcgc cataccggta
    15601 ctttgcacca gaatcatttc tggttcgaaa ggatcgtcca gccgttcgcg ttcgacgata
    15661 aactccatca acgcttccag cacgtccaga cgattggaat ggtagaccct taacatagcg
    15721 gctcctgact actgacgatt cgggcaatgc aggcgggtca tctcgccttc tctgcccccg
    15781 ggtgaaacta gcgtaacgct gatgctgaca catcccgcct gcgatgtctg cattcggttg
    15841 acctgccagt tggcaggtgg cgaaatcgcg cgcagttgcg tttgctgcca gccatgccgc
    15901 cagagctgtt ggtactggtt tctgctggca agactgttca ttaatgttcg ctggaaaccc
    15961 gataacgcag tgacaatcat caccatcaac accatcgcca acattacctc cggcaggcta
    16021 aagccttgtt gattcttcag ggaagctgac ataacgccct ctctttcagc ggacaaaaat
    16081 cgctccagcc gcatggcgaa aaaacaatgt ttccatcgat gacttcgcct gttcgccaca
    16141 acgaaacgcc ttcataaccg gcaatcaata aggcttcatt atctaccaat aaacgcagac
    16201 aaacccgggc accggtggca gcgtacagta agcactgaac tgcgggctgc gtctgccagg
    16261 agtgcatttt tccccactcc agcgccgact gaacgatggc ctggcggcgc aatgactgac
    16321 tttccatgct cacgcgagaa gcaaaactgc gatcctgttg gctcattcct tgtaatagca
    16381 agctacccaa aatcagcagc atcaggacca gagccagtga cgaaacaccc ttttcgcggt
    16441 tcacaggttg aatcctgtca cgctatagct ggcatccacc acagtttgcg gatcagcctt
    16501 gctggcagcg tgcatattaa ccgtcaacac cggcgagaaa ccactgacat cctgtcgggt
    16561 aacctgaaaa gtgtcgataa tgatggcatc cggattagtg actttatccc agcccttatc
    16621 ttcacaggat gtcgcaccgc gtagcgtttc cagcacatgc tccttcagac gaaatccaat
    16681 ctggtcggac tcttttaccg gttcgcgatc ccagataccg ttactgttcg catcccactg
    16741 cacaatgaca cagtcaccct gtccgacaat ttccagccct tcgccggtac agctgccatg
    16801 acaataaccc gccctctgga gatgcttcgc aacggtaaat acccgcagcc agatttcatc
    16861 ttccagcgcc agcttacggg tgctcgttaa actgtcacgc tgtaacgcag gcagaaagcg
    16921 tgcagccccc agcaacaata cgctactgat cgccatagca atcaacactt ccagcagaga
    16981 aaaaccttgc tcttttacag gcatccttct gtttctcctt gctgacaaag ccggagtctt
    17041 ccccacggcg aaaccaccag ccaccactcg cccgttgagt ttttgaagcg aatatgcccg
    17101 gcccatgcgg tattgcgcag gccaaagaaa gcaagcgaag gtgtcagatc gctcatttcg
    17161 acttcgggcc agcgtggcac aaagaccaat ggtgaactgc catgacaggt attggcccca
    17221 gcagcggaac tcacaaggca ccataacgtc ccctccctga taacactgat actgtggtcg
    17281 cggttatgcc agttggcatc ttcacgtaaa tagagcaaat agtcccgcgc ctggctggcg
    17341 gtttgccaaa gccgttgcga ctgctgccag tattgccagc catagagtcc acttgcgctt
    17401 agcatgacca gaatcagcat cgcgaccagc gtttcaatca gcgtataacc acgttgtgtt
    17461 ttcatgccgg cagtatggag cgaggagaaa aaaagacgag ggccagtttc tatttcttcg
    17521 gcgcatcttc cggactattt acgccgttgc aggacgttgc aaaatttcgg gaaggtgtct
    17581 cgaagaattt aacggagggt aaaaaaaaac cgacgcacac tggcgtcggc tctggcagga
    17641 tgtttcgtaa ttagatagcc accggcgctt taatgcccgg atgcggatcg tagccttcaa
    17701 tctcaaagtc ttcgaaacgg tagtcgaaga tggattcggg tttacgtttg ataatcaact
    17761 tcggcagcgg acgcggttcg cggcttaatt gcagatgagt ttgatccata tggttgctgt
    17821 acaggtgcgt atcgccaccg gtccagacaa aatcacccac ttccagatcg cactgctgcg
    17881 ccatcatatg caccagtaac gcgtagctgg caatgttgaa cggcaggccg aggaagacgt
    17941 cacaggagcg ctgataaagc tggcaagaga gtttgccgtc tgccacatag aactggaaga
    18001 atgcatggca cggtgccagc gccattttat ccagttcgcc tacgttccac gctgaaacaa
    18061 taatgcggcg ggaatccggg tcgtttttca gctggttcaa taccgtagtg atctggtcaa
    18121 tatgacgacc atctggcgtt ggccaggcgc gccactgttt accatacact ggcccgaggt
    18181 cgccgttttc atcggcccat tcgtcccaga tggtgacatt gttttcgtgt agataagcaa
    18241 tgttagtgtc gccctgcaga aaccacagca gttcatggat gatggaacgc aggtggcaac
    18301 gtttagttgt caccagcggg aatccatcct gcaggttaaa acgcatctga tgaccaaaaa
    18361 tggaaagcgt tccggttccg gtacggtcgt ttttctgtgt gccttcgtcg agcacttttt
    18421 gcatcagttc taaatactgt ttcatggttc ctcaggaaac gtgttgctgt gggctgcgac
    18481 gatatgccca gaccatcatg atcacacccg cgacaatcat cgggatggaa agaatttgcc
    18541 ccatgctgat gtactgcacc caggcaccgg taaactgcgc gtcgggctgg cggaaaaact
    18601 caacaatgat gcgaaacgcg ccgtaaccaa tcaggaacaa gcctgagact gctcccattg
    18661 ggcgcggttt acgaatatac aggttgagga taataaacag caccacacct tccagcagca
    18721 gctcgtaaag ctgtgatggg tggcgcggca gcacaccgta agtgtcgaaa atggattgcc
    18781 actgcgggtt ggtttgcagc agcaaaatat cttctgtgcg ggagccaggg aacagcatgg
    18841 caaacgggaa gttcgggtca acgcggcccc acaattcacc gttaataaag ttgcccagac
    18901 gcccggcacc aagaccaaac ggaatgagtg gggcgataaa atcagagacc tggaagaagg
    18961 aacgtttagt acggcgggcg aagataatca tcaccacgat aacgccaatc aggccgccgt
    19021 ggaaagacat gccgccgtcc cagacacgga acagatacag cggatcggcc ataaactgcg
    19081 ggaaattgta gaacagaaca taaccaatac gtcccccgag gaagacgccg aggaagcccg
    19141 catagagtaa gttttcaact tcatttttgg tccagccgct gcccggacga ttcgcccgac
    19201 gtgttgccag ccacattgca aaaatgaaac ccaccagata catcaggccg taccagtgaa
    19261 gcgctacggg tcctattgag aaaatgaccg gatcaaactc cggaaaatgc agatagctac
    19321 tggtcatctg tcaccacaag ttcttgttat ttcgctgaaa gagaacagcg attgaaatgc
    19381 gcgccgcagg tttcaggcgc tccaaaggtg cgaataatag cacaagggga ccaggctggt
    19441 tgccggatac cgttaaaaga tatgtatatg atccgcgcta taaccctccg cgaatcagcc
    19501 cgcccatgcc gcgacgctcc ataaaggctg caacctgatg gcgaacttcg gtcgccagtt
    19561 gcgcttccag actacgctgc gcaagatttt ctgcttcggc aaaatcaatg cgccgcagca
    19621 ggtattttac gcgcgctaca gaacgtccgt tcatagacag atggcgatac ccaagcccaa
    19681 tgaggattgc cacgcacatg ggatcgcccg ccatttcacc gcacaaacgg agatcgattc
    19741 catgtatttc cgcttcccgg gcgatcatcg ccagagctcg taacattgca ggatgaagac
    19801 tgtcataaat gttcgccacc cgggtattgt tgcgatcaac ggccaggatg tattgagtca
    19861 gatcgttggt gccaacagag ataaaatcga cccgctttgc cagatgcggc agcataaata
    19921 ccattgacgg cacttccagc atgatgccga tacgtggttt gggaatttcg taaccgatca
    19981 tctcctcgac ttcacgtcct gcacgttcaa tcaggcggcg tgcttcgtca acttcatcga
    20041 ggctggtgac catcggcaac agaatattca ggttgcccgt agcggcatta gcacgcagca
    20101 tcgcccgcac ctggatcaag aagatctccg gctgatcgag cgtaatgcga atcccacgcc
    20161 aacccaggca tggattctct tcgctgatcg gcatgtaagg cagctgctta tctgctccga
    20221 catccagcgt acgcaaggtg acgggtttat cattgaacat ttgcagcatc ccctgatact
    20281 gcgccacctg ttcttcttcc gacggaaaac cactttgcag catgaatggg atttcagtgc
    20341 gataaagtcc tatgccatca atacggctgc ccagtttttc ttcatgttcc gggcttaaac
    20401 cggcattgag catgacttta atgcgctcgc cgctttttaa ctgggcgggt aaattgacgt
    20461 catcttccgc cagacggcta agctcaatct cttcactaat tagccgctga tattcttgca
    20521 gcagtaccgg ctccggatcg accagcaatt caccgcgata accatcgacg atcaacgtcc
    20581 gacgatgcag caccgaaggc tgaatatccg cgcccattac ggtagggatc cccagcgcac
    20641 gtaccatgat cgcagcatgg gagttggctg cgccatctcg cacgacaaca ccgactaagc
    20701 gatcctgggg cagctctgca agcgttgtcg ctgacagttc atctgccacc agaatgaaac
    20761 gttccggcca ggcgttcggc ccttgattag cgtcatcaag atgaaacagc aatcgctgac
    20821 ccagcgcacg taaatcgcca gcccgctctt tgagatagtt atcgcttagc gcggcaaact
    20881 gttcggcaaa tttttcaatg accgttttta ccgcccactc tgccaccgag cctttatcaa
    20941 cctcggcaaa caattcgcga cgcagccggg tgtccgaaag caggtgcgag taaagatcga
    21001 aaatagccgc cgtttctttt tgtgcaccgg cggcaaagcg tttgctgtag cggcgaaact
    21061 cgtttgccgc ttcttccagc gccccggtca gtcgttcgcg ttccagagcc ggatccaacg
    21121 ttgatgcctg atacacctgt tccattaaag gtaacgtggc atcctgccag ccttcggcaa
    21181 tcgccacacc aggtgctgcc ggtaatgcac ggattcgcgt ctggcgatat tgcccaaaca
    21241 aggcagtcaa ctgcgactga gaaagaatcg ctgccatctg ggtggcaagc gtcaccagga
    21301 aggattcttc actttcgtca tactggcgca gctctcgttg ctgtaccacc agtacaccaa
    21361 gcaactggcg acgttgaata attggtacgc ctaaaaacgc gcggaaacgt tcttctttta
    21421 cggaggggat gtatttgaag ctggggtgct tttgcgcatc tgcaaggttt atcggttccg
    21481 ccagcctgcc aaccaggccg acgatccctt catcaaacgc gagcgttaca gtgcgaccgc
    21541 gtggtttttt cagcccccgg gtcgccatca ggtagtaaca acgtcgatca tgatcggcca
    21601 ggtagaccga acagacctcg gtatccatcg caagacagat gtcggtaacc agaatattta
    21661 acgcctcatt caggcgtggt gcgctggcta ccttttcgac tatttcgcgc aggcgagtga
    21721 gcataattgg cgtgacttaa cctcttttac gtcgataagc agatgcgttt tgtggttttg
    21781 gcgtattttc ctgcagtgac atcaccacac tcgcgaactc tttcattacc ctacggtaga
    21841 catcacgttt aaatgacacc acctgtctga ctggatacca gtaacttacc catcgccagc
    21901 cgtcaaactc tggtgtactg ctggtttgca tattgatttc tgcatcgccg ctcaccagct
    21961 gcaagagaaa ccatttttgt ttttggccga tacaaaccgg cttcgtgtcc caacgcacca
    22021 aacgtttcgg taatttgtag cgcaaccagt tacgcgttga agcaaggatt cgaacgtctt
    22081 tgcggcttaa tcctacttct tcaaacaatt cacggtacat cgcctgctct gcggattctc
    22141 cggggttgat tccgccttgc ggaaattgcc aggagtgctg accaaatcgc cgggcccaca
    22201 ttacctgccc ctggcgatta caaatcacga tacctacgtt tgggcggtag ccatcgtcat
    22261 caatcaccgg actacctcaa aataaagctt tatatacgaa tgattgtttc atactccagg
    22321 aagacggtaa accactctct gcagggcatt acacactaat aacaattgaa taactcacag
    22381 ttatgtgcag agttataaac agaggaaggg gtggatagcc gtttttattc accttttctg
    22441 tggatagagt tgtgaagaac tacggaatta ctacgggaaa acccggagca ttctgaataa
    22501 gcccgatata gacattttaa aatattcatt atcagtcaat gcattacatg taatttgagt
    22561 aataaaaaag agtaattcgt gacccaggtc acacctctca tttacgggtt ggcgaaaaat
    22621 caatcaatgc ggtatttatc cacagaatgt gccactaagt taagcactga gccactaaaa
    22681 actggagttt cgtcgcacgt caaggctgta aatggaaaca gtagtggagg tttttcacag
    22741 ttatcccagc tttctgtgga taacatggtg taagatcctg tttattttca gtgaccagat
    22801 ttggaaaacc cgttgcagtg ttgcgcaact cgattaccga caatctaaaa agcaatataa
    22861 atcagataat tgaacaacgc atgtggaaaa agttacactg cgaatattcg gcacagaaat
    22921 gctgtttgtt ttttaaccaa ggtatcatga catgtcccaa cctcgcccac tgctctctcc
    22981 tcccgaaact gaagaacaat tgttagcgca agcacaacaa ctttctggtt atacattggg
    23041 agaactggcg gcacttgctg ggctggtgac gccggagaat ttaaaacgcg ataagggctg
    23101 gattggcgtg ttactggaga tctggctagg tgccagcgca gggagtaaac ctgagcaaga
    23161 ttttgctgcc ctgggcgtgg aacttaaatc gaacaattac gtctgttctt tgaatccagc
    23221 ctggtgcgct atgcggctga acacgccacc gatgaacaaa tcgatttgct ggcaaaagca
    23281 ctggaaatca acagtcagtc gctggataac aacgcggcat tcattcgctc agacgttgat
    23341 ttccaccgcg ttctggcgga gatccccggt aacccaatct tcatggcgat ccacgttgct
    23401 ctgctcgact ggctgatcgc cgcacgtcca accgtggctg atcaggctct gcacgagcat
    23461 aacaacgtta gctatcaaca gcatattgcg atcgttgacg cgatccggcg tcatgatcct
    23521 gacgaagccg atcgtgcgtt gcaatcgcat ctcaacagcg tctctgctac ctggcacgcc
    23581 ttcggtcaga ccaccaacaa aaagaaataa tgccacttta gtgaagcaga tcgcattata
    23641 agctttctgt atggggtgtt gcttaattga tctggtataa caggtataaa ggtatatcgt
    23701 ttatcagaca agcatcactt cagaggtatt tatggcaacg aatttacgtg gcgtaatggc
    23761 tgcactcctg actccttttg atcaacaaca agcactggat aaagcgagtc tgcgtcgcct
    23821 ggttcagttc aatattcagc agggcattga cggtttatac gtgggtggtt cgaccggcga
    23881 agcctttgta caaagccttt ccgagcgtga acaggtactg gaaatcgtcg ccgaagaggc
    23941 gaaaggtaag attaaactca tcgcccacgt cggttgcgtc agcaccgccg aaagccaaca
    24001 acttgcggca tcggctaaac gttatggctt cgatgccgtc tccgccgtca cgccgttcta
    24061 ctatcctttc agctttgaag aacactgcga tcactatcgg gcaattattg attcggcgga
    24121 tggtttgccg atggtggtgt acaacattcc tgccctgagc ggggtaaaac taaccctgga
    24181 tcagatcaac accctggtta cgctgcctgg tgtcggcgcg ctgaaacaga cctctggcga
    24241 tctctatcag atggaacaga tccgtcgtga acatcctgat ctggtgctct ataacggtta
    24301 cgacgaaatc ttcgcctctg gtcttctggc gggcgctgat ggtggtatcg gtagtaccta
    24361 caacatcatg ggctggcgtt atcaggggat tgtgaaggcg ctgaaagaag gcgatatcca
    24421 gaccgcgcag aagctgcaaa ccgaatgtaa taaagtcatt gatttactga tcaaaacggg
    24481 cgtattccgc ggcctgaaaa cggtcctgca ttatatggat gtcgtttctg tgccgctgtg
    24541 ccgcaaaccg tttggtccgg tcgatgaaaa gtatctgcca gaactgaagg cgctggccca
    24601 gcagttgatg caagagcgcg ggtgagttgt ttcccctcgc tcgcccctac cgggtgaggg
    24661 gaaataaacg catctgtacc ctacaatttt cataccaaag cgtgtgggca tcgcccaccg
    24721 cgggagactc acaatgagta ctacaaccca gaatatcccg tggtatcgcc atctcaatcg
    24781 tgcacaatgg cgggcatttt ccgctgcctg gttgggatat ctgcttgacg gttttgattt
    24841 cgttttaatc gccctggtac tcaccgaagt acaaggtgaa ttcgggctga cgacggtgca
    24901 ggcggcaagt ctgatctccg cagcctttat ctctcgctgg ttcggcggcc tgatgctcgg
    24961 cgctatgggt gaccgctacg ggcgtcgtct ggcaatggtc accagcatcg ttctcttctc
    25021 ggccgggacg ctggcctgcg gctttgcgcc aggctacatc accatgttta tcgctcgtct
    25081 ggtcatcggc atggggatgg cgggtgaata cggttccagc gccacctatg tcattgaaag
    25141 ctggccaaaa catctgcgta ataaagccag tggttttctg atttccggct tctctgtggg
    25201 ggccgtcgtt gccgctcagg tctatagcct ggtggttccg gtctggggct ggcgtgcgct
    25261 gttctttatc ggcattttgc caatcatctt tgctctctgg ctgcgtaaaa acatcccgga
    25321 agcggaagac tggaaagaga aacatgctgg taaagcacca gtacgcacga tggtagatat
    25381 tctctaccgt ggtgaacatc gcattgccaa tatcgtaatg acactggcgg cggctactgc
    25441 gctgtggttt tgcttcgccg gtaacctgca aaatgccgtg atcgtcgctg ttcttgggct
    25501 gttatgcgcc gcaatcttta tcagctttat ggtgcaaagt acgggcaaac gctggccaac
    25561 gggcgtaatg ctgatggtgg tcgtgttgtt tgctttcctc tactcatggc cgattcaggc
    25621 gctgctgcca acgtatctga aaaccgatct ggcttataac ccgcatactg tcgccaacgt
    25681 gctgttcttt agtggctttg gcgcggcggt gggatgctgc gtaggtggct tcctcggtga
    25741 ctggctggga acccgcaaag cgtacgtttg tagcctgctg gcctcgcagc tgctgattat
    25801 tccggtattt gcgattggcg gcgcaaacgt ctgggtgctc ggtctgttac tgttcttcca
    25861 gcaaatgctt ggacaaggga tcgccgggat cttaccgaaa ctgattggcg gttatttcga
    25921 taccgaccag cgtgcagcgg gcctgggctt tacctacaac gtcggcgcat tgggcggtgc
    25981 actggcccca atcatcggcg cgttgatcgc tcaacgtctg gatttgggta ctgcgctggc
    26041 atcgctctcg ttcagtctga catttgtggt gatcctgctg attggtctgg atatgccttc
    26101 ccgcgttcaa cgttggctgc gcccggaagc attgcgtact catgacgcta tcgacggcaa
    26161 accatttagc ggtgccgtgc catttggccg cgccaaaaac gatttagtca aaaccaaaag
    26221 ttaatcctgt tgcccggtct ctgtaccggg cctttcgcta agggaagatg tatgtcgtta
    26281 cttgcacaac tggatcaaaa aatcgctgct aacggtggcc tgattgtctc ctgccagccg
    26341 gtccctgaca gcccgctcga taaacccgaa atcgtcgctg ccatggcatt agctgcagag
    26401 caggcgggcg cggttgccat tcgcattgaa ggtgtggcaa atctgcaagc cacgcgtgcg
    26461 gtggtgagcg tgccaattat cgggattgtg aaacgcgatc tggaggattc tccggtacgc
    26521 atcacggcct atattgaaga tgttgatgcg ctggcgcagg cgggcgcgga cattatcgcc
    26581 attgacggca ccgaccgccc gcgtccggtg cctgttgaaa cgttgctggc acgtattcac
    26641 catcacggtt tactggcgat gaccgactgc tcaacgccgg aagacggcct ggcatgccaa
    26701 aagctgggag ccgaaattat tggcactacg ctttctggct ataccacgcc tgaaacgcca
    26761 gaagagccgg atctggcgct ggtgaaaacg ttgagcgacg ccggatgtcg ggtgattgcc
    26821 gaagggcgtt acaacacgcc tgctcaggcg gcagatgcga tgcgccacgg cgcgtgggcg
    26881 gtgacggtcg gttctgccat tacgcgtctt gaacacattt gtcagtggta caacacagcg
    26941 atgaaaaagg cggtgctatg accacactgg cgattgatat cggcggtact aaacttgccg
    27001 ccgcgctgat tggcgctgat gggcagatcc gcgatcgtcg tgaacttcct acgccagcca
    27061 gccagacacc agaagccttg cgtgatgcct tatccgcatt agtctctccg ttgcaagctc
    27121 atgcgcagcg ggttgccatc gcttcaaccg gaattattcg cgacggcagt ttgctggcgc
    27181 ttaatccgca taatctcggt ggattactac actttccgtt agtcaaaacg ctggagcaac
    27241 ttaccgattt gccgaccatt gccattaacg acgcgcaggc cgcagcatgg gcggagtatc
    27301 aggcgctgga aggcgatata accgagatgg tgtttatcac tgtttccact ggcgtcggcg
    27361 gcggtgtagt gagcggcggc aaactgctta ccggccctgg aggtctggcg gggcacatcg
    27421 ggcatacgct tgccgatccg catggtccag tctgcggctg tggacgcaca ggttgcgtgg
    27481 aggcgattgc ttctggtcgc ggcattgcgg cggcggcgca aggggagttg gctggtgcga
    27541 atgcgaaaac tattttcacg catgccgggc agggcgatga gcaggcacag caactgattc
    27601 accgttccgc acgtacgctt gcaaggctga tcgctgatat taaagccaca actgattgcc
    27661 agtgcgtggt ggtcggtggc agcgttggtc tggcagaggg gtatctggcg ctggtggaaa
    27721 cgtatctggc gcaggagcca gcggcatttc atgttgattt actggcggcg cactaccgcc
    27781 acgatgcagg tttacttggg gctgcgctgt tggcccaggg agaaaaatta tgatgatggg
    27841 tgaagtacag tcattaccgt ctgccggatt gcatcctgcg ttacaggacg cgttaacact
    27901 agccttagcc gccaggccgc aagaaaaagc accgggtcgt tacgaattac agggcgacaa
    27961 tatctttatg aatgtcatga cgtttaacac tcaatcgccc gtcgagaaaa aagcggaatt
    28021 gcacgagcaa tacattgata tccagctgtt gttaaacggt gaggaacgga ttctgtttgg
    28081 cacggcaggc actgcgcgtc agtgtgaaga gttccaccat gaggatgatt accagctttg
    28141 cagcgccatt gagaacgagc aagccatcat cttaaaaccg ggaatgttcg ccgtgtttat
    28201 gccgggagaa ccgcataaac caggatgcgt tgtcggcgaa cctgatgaga ttaaaaaggt
    28261 tgtggtgaag gttaaggctg atttaatggc ttaaaactta gttcgtttta aagattctgc
    28321 agacactgtc tgcagaattc gaaaccgctg aaattaacgg cgtctatagc cttcctctag
    28381 caccgcctgt gttcgaacga gttcatccgc gagtcgtttt tcatcaggta gttgcatctt
    28441 atattcgctc gccagaacgg tgttaggcaa acctgccaga gcataatgtg cttctccggc
    28501 tcctttctct gcacagagaa ccagaccgat gggcggattt tcatccggta gcgtccagtg
    28561 ctctttggcg tagttgagat acatattcat ctgtccggca tcgctatagc tgaatttgcc
    28621 cacttttaga tcgacgatta gcaggcagcg taaacggcgg tggaaaaaca gcagatcgac
    28681 acgaaaccag ttgtcatcaa tgcgtaacct tcgctgtcga ccaacaaagg caaaatcatc
    28741 cccaagttcc agcatgaaat ccatcaggtg gttgatcagc gcctcctcaa aatcggattc
    28801 tgagtattca tctttcaatt ccagaaattc gagcacaaag ggatcgcgta tcgcctgctg
    28861 cggaagaata tgcgtctcgg taggagcgtg ttgttgcagc atggctgatt tgtcatgtga
    28921 cagtagtgtc cgctcataaa actgggtcgc aatttgtcgt tcgagctgac gagcagacca
    28981 gccacagcgg agtgtctctt tctcataaaa gctgcgagca tcagcttttt taacagaaag
    29041 caaacggacg taagtggacc agggcagggg gaaggatttg gcgagttgcg ctaaatggca
    29101 agattctcca gacacagtct ggagaattgt gggggtgaat tgtgcagaca gtgtctgcac
    29161 aatttcaatt ctttgaaaac aaatataaaa attcttaaac tgccataaat tcctggtgga
    29221 aaatccacgc ttatacctta gacttaaatc ctttgatagt cgcttgatta gctgcgcacc
    29281 atacgcagcc ctggcctcgc caccttgttc aaattcgaca attcgtcggc caatttccca
    29341 gtacgtcgcg gtcattaacg cgttaacgct acgtaccgtt tccgttcggg cgctatcgac
    29401 cagatgaata atgccgtcgt ggatttgctg gtagcctgct gtggttcctt ctgagagcga
    29461 ttccattatc tgtcccttaa tatggcgcag tgttgaataa tggaatcagc tatacgccgg
    29521 gcatctgccg gtaacaatcc agatatgcgt attacattgg ttttcttcag attatgcgag
    29581 gcgctttcca gaacgaaagg agaatttcag cgccgactct tttctgagga ggcggcgctg
    29641 gagcagggat taaacttcca gccagttcat aatgccgtct gccgccttac gaccttcggc
    29701 aatagcggtc accaccagat cggaacctcg gacgatatcg ccgccagcaa agattttcgg
    29761 gttgctggtc tggaaagcgt tgtcgttgcc ttccggagcg atgatgcggc cttgggaatc
    29821 cagctcgacg ctgtgttttg ccagccattc catgctgtgc gggcgaaagc caaacgccat
    29881 gatcaccgca tctgcaggaa cgatatgctc tgaccctgcc acgatctctg cgcggcgacg
    29941 gcctttggca tccggttcgc ccatttcggt acgcaccatt tttacgccgc tgactttgcc
    30001 gttaccgttc acttcaatcc ccagtggctg gacattgaat ttgaactcta caccttcttc
    30061 ccgcgcgttt ttcacttcgc ggcgggaacc tggcatgttc tcttcatcac gacgataggc
    30121 acaggtaacg tgcttcgctc cctggcgtac agacgtacgc acgcagtcca tcgcagtgtc
    30181 gccaccgcca agtaccacca cgcgtttgcc ttccatgctg acgaacggtt cttcgcgggt
    30241 ttcgccaaag cccattaact gtttggtgtt ggcgataagg aacggcagcg ctgcgtacac
    30301 gccatcggcg tcttcgtttt ccagcccgcc gcgcattgac tgataagtcc cgacgccaag
    30361 gaacacggca tcgtaatcac tcagcagatc gtccagctgt acgtcgcggc ccacttcggt
    30421 attgagtttg aattcaatac ccatgccggt gaagatttca cggcggcgcg tcattacctc
    30481 tttttccagc ttgaaagccg gaataccgaa ggtcagcaat ccgccaattt ccggatgacg
    30541 gtcgaagaca accgctttta cgccgttgcg cgtcagcaca tccgcacacg ccagacccgc
    30601 cgggcctgca ccgataatcg ctactttttt accggtctgt ttcacgccag atatatccgg
    30661 acgccagccc atttcgaacg ctttatcatt gatataacgc tcaatgttgc cgatggtcac
    30721 tgcgccaaac tcatcgttca gggtgcagga accttcgcac agacggtctt gcgggcagac
    30781 acgtccgcac acttccggca gggtattggt ctgatgcgac agttccgccg cttcaaaaat
    30841 acgcccctcg ttggcgagct tcagccagtt cgggatgtag ttgtgtaccg ggcatttcca
    30901 ctcgcagtat gggttgccgc acgacaggca gcggtcagcc tgcgctttgg cctggccttc
    30961 ggaaaacggc tcgtaaattt caacaaactc aattttgcgg atcttcagcg gtttctttgg
    31021 cggatcaacg cgctgcaggt cgataaattg ataaacattc tgactcattg ttgctacccc
    31081 ttactgcgcc tgcacgcgca actctgctgc gctacgacta cggtgaccca gcagtgcttt
    31141 tacatcactg gacttcggtt taaccagcgc aaatttagtg gcgaaggttg accagttcgc
    31201 cagaatctct tcaccacgct gagagccagt atgctgaaca tgctcggtga taagaccacg
    31261 cagatgctct tcatggatcg ccagatcgtc aacgcttaag acctcgacca gttccgggtt
    31321 aacgcgtttg cggaaatcgc cgctttcatc gagaacgtaa gcgaagcctc cggtcatacc
    31381 cgcgccgaaa ttcacgccgg ttttacccag aatacagaca ataccgcccg tcatatattc
    31441 acagccattg tcgccaatgc cttctaccac ggtgatagca ccggagttac gtacgccgaa
    31501 acgttcaccc gcgcggcctg cggcatacag acgacctccg gtcgcgccat acaggcaggt
    31561 gttgccgata atgcttgctt catggctgcg gaaggctgag ccaaccggag ggcgaatggc
    31621 gattaagccg cccgccatgc ctttaccgac atagtcgttg gcatcaccgg tcaggtacag
    31681 ttccacgccg cccgcgttcc acacgccgaa gctctggcct gcggtgccgt tgaagtacgc
    31741 cttgatcgga tcggcagcca gcccctgatc gccgtgcgtc tgggcgatat agcctgaaag
    31801 cgatgcgccg acagaacggt cggtgttgcg gatatcgaac cagaaggttt tgctctggcg
    31861 ttcatcgaca aacggttttg cctgttgcag caactgcgcg ttcagtaggc cgttatcaaa
    31921 cggcgggttg ttttcggtgc agtagagtgc cttacctgca tgtggctcgg cagtttccag
    31981 cagcttcgac agcgccagtt tctgctgttt ggcggtgaaa ccgtccagct ctttcagcag
    32041 gtcggtgcga ccaatcagat ccaccagacg cgttaccccc agctgtgcca tcagctcgcg
    32101 ggtttcacgt gcgataaact caaagtaatt cgtcaccttg aacggcaggc cgtgatagtg
    32161 attcttacgc agtttgtcat cctgagttgc aacacccgtc gcgcagttgt tcagatggca
    32221 aatacgcaga tatttacagc cgagcgccac catcgggcca gtgccgaagc cgaagctttc
    32281 tgcgccgaga atcgccgctt tgatgatatc aacgccggtt ttcaggccgc catcaacctg
    32341 caaacggatc ttatgacgca gaccattggc aaccagcgcc tgttgggttt caacaagccc
    32401 cagctcccac ggacagcctg cgtatttcac cgatgaaagc ggacttgcgc cggtgccgcc
    32461 atcatagcct gcgatggtga ttaaatcggc ataagctttc gccacgccag tcgcgatggt
    32521 gcctactccc ggttcggaaa ccagcttcac ggagatcatc gctttcgggt taacctgctt
    32581 gaggtcgaaa ataagctgcg ctaagtcctc gatagagtag atatcatggt gcggcggcgg
    32641 ggagatcagc gtcactccgg gcaccgaata gcgcagtttg gcgatgtaag gcgtgacttt
    32701 atcgcccggt aactgaccgc cttcgcccgg cttcgcgccc tgggcgactt taatctgaat
    32761 gacgtcggca ttgaccagat acgccggagt aaccccaaag cgaccggaag ccacctgctt
    32821 gatgcgcgac actttgttgg tgccgtagcg tgccggatct tcgccgcctt caccggagtt
    32881 agagttgccg ccgatgctgt tcatcgcttc agccagcgcc tcgtgggctt ccgggcttaa
    32941 cgcgccgata gacatcgcag cggtatcgaa gcgtttaaac agttcgcttg ccggttcaac
    33001 atcagcaatg ttgaccgcgt tttcacccgg cgtaattgcc agcagatcgc gcagcgtggt
    33061 tgccggacgc tcattaacca gcttcgcgta ttcctgatag tcgctgtact cgccgctttg
    33121 taccgcctgt tgcagcgtgc gcaccacgtc cgggttgtag gcgtggtatt cgccgccgtg
    33181 cacatatttc agcaggccgc cctggctgat gggcttacgc gccagccagg cacgtttcga
    33241 cagattcagc agatcctgct ggaagtcttc aaagcttgcc ccgccaatgc ggctgaccgc
    33301 gccctggaag cacaggccta cgacatcatc gtgcagaccg accgcttcaa acagtttcga
    33361 gcagcggtaa gaggcgatgg tggagatgcc cattttggac atgattttgt acaagccttt
    33421 gttgatgccg ttacggtagt tgagcatcac ggtacgataa tctttggcaa tcgcgtgggt
    33481 gtctaccagg cggcccagcg tttcataggc aaggtacgga taaatagccg tcgcaccgaa
    33541 gcccagcaac acggcgaaat ggtgcggatc gcgggcgctg gcggtttcga cgatgatgtt
    33601 ggcatcgcaa cgcaagcttt gatcgaccag acgggtctgg atcgcgccaa ccgccatcgg
    33661 ggctggaacc ggcaggcgat ctttagcgat attcctgtcg gagagcacca gcagcacagt
    33721 gccgctacgt accatttttt cagctttgtc gcacagctct ttgaccgtcg cttcgagcgt
    33781 ggttttggtg acgtcaaagg tgatatccag tgtatctgcg cggtagtgct cctctttcat
    33841 cgtcgtgagc tgtttgaaat cggagtagag cagaatcggc gatttaaaac ttaaacggtg
    33901 cgcctggccc tctgcttcac agaagacgtt catttcacga ccaatgctgg tggcgagcga
    33961 cataacatgc gcttcacgca gcgggtcgat tggcgggtta gtcacctggg caaactgctg
    34021 gcggaagtag tcgtaaataa tgcgcggctg actggagagc acggcgaatg gggtgtcgtc
    34081 gcccatcgaa ccgaccgctt cctgaccgtt ttcgcccagt acgcgaatta cggagtccag
    34141 ctcttccgcg ctgtagttaa actgtttctg gtaactggca agtgtgtcgt cgtccagttc
    34201 gcggctacct acttcttcat cgggcaggtc ttcaaacggt accagtcggc ggacgttttt
    34261 ctccatccac tctttatatg gatgacggct tttcagatcg tcatcggttt ctgccgagtg
    34321 cagaatgcgc ccactgcggg tgtcgataac catcagttcg cctggcccca cgcggccttt
    34381 ttcgaccact tcgtcaggct gataatccca gataccaact tcagaggcgc aggtgatgag
    34441 cttatctttg gtgatgacat agcgtgccgg acgcagaccg ttacggtcga ggttacaggc
    34501 ggcaaaacga ccgtcagaca tcacgatgcc cgccgggcca tcccacggct ccatatgcat
    34561 ggagttaaag tcaaagaagg cacgcagttc cgggtccata tccggattgt tctgccaggc
    34621 gggtggtact aacagacgca tggcgcggat gatatccatc ccacctgcca gcagcagttc
    34681 cagcatgtta tccatggaac tggagtcaga gccggtttcg ttgacgaacg gcgcggcgtc
    34741 gtgcaggtca gggatcagcg gcgtctggaa tttataggta cgtgcgcgcg cccattggcg
    34801 gttaccggtg atggtgttga tttcaccgtt atgcgccagg tagcggaacg gctgcgccag
    34861 cggccagcgc ggtacggtgt tagtggagaa gcgctggtgg aacaggcaaa tggccgattc
    34921 cagacgcagg tccgcaagat ccagataaaa gcgcggcaga tccgccggca tacacagacc
    34981 tttataaata ttgaccagat tcgacaggct acagacgtag aagtctttgt cggcttcgag
    35041 acgcttttca atgcggcggc gggcgataaa cagacggcgc tccatatcgc gcggacgcca
    35101 gcctgccggg gcgttcacaa aaatttgctc aatgcgtggc agagaggaga gggcgatttc
    35161 acccagcacg ccttcgttag tggggacatc acgccagccc acaatcgaca acgtttcgcg
    35221 ttgcagctct tcttcaacga tgcggcgtgc ggcagcagcg agttcaggat ctttattcag
    35281 gaagagcatc ccgacagcgt agttttttgc taaacgccat ccgcgctcct gcgcaacgat
    35341 gcgaaaaaag cgatccggtt tttgtaacag caagccgcaa ccgtcaccgg ttttaccatc
    35401 ggcgagaatc gcgccacggt gctgcatgcg ggccagtgcg tgtattgcag tacgcactac
    35461 cttgtggcta ggttcgcctt ctatgtgggc gatcaggccg aaaccacagt tatccctctc
    35521 aagggattta tcgtacaaca tatcgtgaac ctcccccagg ctctgcggga accccacttc
    35581 cgaaccgctg cgcacgggca cagaaagagc gtggcgacgg ggtttgcgtg tcatacgcgc
    35641 gcctcgcatt cgcccccttg ttgtcctttt cgcatcggtt aatacggtga agaggggatc
    35701 tcaattactg cataaatatg atgagcaggc tgctcatcca gaaagcttcc agcggatttc
    35761 caacttatcg ggaaagctga tacaggtcaa atggcaagct tattggtaca gaaatgtgct
    35821 ttagcgcaaa attctttatt aaatggctga aattaaagtg gattttatct ttaaggctaa
    35881 aaggggagtc gttagagaaa cagtcaattc ctgactgtca taaaggcgtg ttaaccctta
    35941 tcctgccttg aactgacgag aaaaccagca ttttatactg ccttaattgg taatgacgtt
    36001 tcatgctact gtttttgcct aaaatccata aggagataaa aaaatctgac acaagataag
    36061 caaaagtgat ttatgtgatt atgcatgaaa tttcggtaat tttgattgat tatgcagttg
    36121 ataaaagcag gccgggacga ttgatccagg tcatcgccga attgagctaa aaatggcagg
    36181 ctttggcctc ttttcaaggc gcggtctatc agattatgca gttacagaaa ttagtcaata
    36241 tgtttggtgg tgatctcacc cgtcgttatg ggcaaaaggt gcataagctg acgctgcacg
    36301 gcggttttag ctgccctaac cgtgacggta ccatcgggcg tggtggctgc acattctgta
    36361 atgtggcctc gtttgccgat gaagcgcagc aacatcgttc cattgccgaa caactggcgc
    36421 accaggcgaa tttagttaac cgcgctaaac gctatctggc ctactttcag gcgtatacca
    36481 gcacctttgc ggaagttcag gtgctgcgtt ctatgtatca gcaggcggtg agccaggcca
    36541 atattgtcgg tttgtgtgtt ggtacccgcc cggactgcgt gccggatgcg gtgctggatc
    36601 tgctttgcga atataaggac cagggttacg aagtgtggct ggagctgggg ctacaaaccg
    36661 ccaacgacaa aacgctgcat cgcatcaatc gcggtcatga ttttgcctgt tatcagcgca
    36721 cgacgcagct ggcgcgtgag cgtgggctga aggtgtgttc tcacttaatt gtcggcctgc
    36781 ctggcgaagg gcaggctgaa tgtttgcaaa cgctggagcg agtggttgaa actggcgtgg
    36841 acggcataaa gctgcatccg ctgcatattg tgaaaggcag cattatggcg aaagcctggg
    36901 aagcggggcg tttgaacggt attgaactgg aggattacac gctcactgcc ggagaaatga
    36961 ttcgccatac gccaccggaa gtgatctacc accgcatttc cgccagcgcc cgtcgcccga
    37021 cgctgcttgc tccgctgtgg tgcgaaaatc gatggacggg aatggtcgag ctggatcgct
    37081 atctgaatga acatggcgta cagggatcgg cgctgggacg tccgtggcta cctccaacgg
    37141 agtagaccat tgaggggaat tccgcatttc tcacacaatt tataacgtaa ctgtcagaat
    37201 tgggtattat tggggcaggt tgtcgtgaag gaattcccta atgaagcaaa ttcgtctgct
    37261 ggcgcagtat tatgttgacc tgatgatgaa gttaggtctg gtgcgcttct caatgttgct
    37321 ggcgctggcc ctcgtcgttc ttgccattgt ggtacaaatg gcggtaacca tggtgctgca
    37381 tggtcaggtc gaaagcattg atgttattcg ttctatcttc tttggtttgc tgattacgcc
    37441 gtgggcggtc tactttctat cggtggtcgt cgagcaactg gaggagtcac gacaacgtct
    37501 gtcacggctg gtgcaaaaac tggaggagat gcgcgagcgc gatttgagcc tcaacgttca
    37561 gttaaaagat aatattgccc agctaaatca ggaaatcgcc gttcgtgaaa aagcggaagc
    37621 agaactgcag gaaaccttcg gccaactgaa aattgaaatt aaagagcgtg aagagacaca
    37681 aattcagctc gaacagcaat cctcattctt acgttccttc cttgatgctt cacccgacct
    37741 ggttttttat cgtaacgaag ataaagagtt ttctggctgt aaccgcgcga tggagctgct
    37801 gaccggaaaa agcgaaaaac aactggttca cctgaaacct gctgatgttt actcaccgga
    37861 agccgccgca aaagtcattg aaaccgatga aaaagtgttc cgtcataatg tgtcactgac
    37921 ctatgaacag tggctggatt acccggacgg gcgcaaagcc tgctttgaaa tccgtaaagt
    37981 gccgtactac gaccgcgtgg gtaaacgtca cggtttgatg ggctttggtc gcgacattac
    38041 cgagcgtaag cggtatcagg atgcgcttga acgggccagc cgcgacaaaa cgacgtttat
    38101 ctccaccatc agtcacgaat tgcgtacgcc gctgaatggt atcgtcggcc tgagccgcat
    38161 tctgctggat accgaactca ccgccgagca ggaaaaatat ctcaaaacca tccatgtttc
    38221 ggccgtcacg ctcgggaata tcttcaacga tattatcgac atggataaga tggaacggcg
    38281 taaggtccag cttgataatc agccggttga tttcaccagc ttccttgccg atctggaaaa
    38341 tctctccgca ttgcaggcgc aacaaaaagg attgcgcttt aatctggagc ctacgctgcc
    38401 attaccgcat caggtcatta ccgacgggac gcgtttacgg cagatcctgt ggaacctcat
    38461 cagtaacgcc gtcaaattca cccagcaagg ccaggttacc gtgcgcgtgc gctacgatga
    38521 aggcgatatg ctgcattttg aagtggaaga ctctggtatc ggcattccgc aggatgagct
    38581 ggataaaatc tttgccatgt attaccaggt gaaagacagt catggcggta aacctgccac
    38641 cggcaccggt attggtctgg ccgtttctcg tcgtctggcg aaaaatatgg gcggcgatat
    38701 tacggttacc agcgaacagg gcaaaggttc aacctttacg ttgacgatcc acgcaccgtc
    38761 ggtggctgaa gaggtcgatg atgcgtttga tgaagacgat atgcctttac cggcgctgaa
    38821 tgtgctgctg gtggaagaca ttgaactgaa cgtgattgtc gcgcgttctg tgctggaaaa
    38881 attaggtaac agcgttgatg tcgccatgac cggcaaggcg gcgctggaga tgtttaaacc
    38941 gggcgaatac gacctggtat tgctggatat tcagttgcca gatatgaccg ggctggatat
    39001 ctctcgtgaa ctgacgaagc gttatccgcg cgaggattta ccgccgctgg tggccttaac
    39061 cgctaacgtg ttgaaagaca aacaagagta cctcaatgct ggaatggatg atgtgctgag
    39121 taagccgctt tctgttccgg tgctaaccgc gatgatcaag aaattctggg atacccagga
    39181 tgatgaggag agtacggtga cgacagaaga gaacagtaaa tcagaagcat tgctcgatat
    39241 tcccatgctg gaacagtatc tcgaacttgt aggaccgaag ctgatcaccg acgggttagc
    39301 ggtgtttgag aagatgatgc cgggctatgt cagcgtgctg gagtcgaatc tgacggcgca
    39361 ggataaaaaa ggcattgttg aggaaggaca taaaattaaa ggtgcggcgg ggtcagtggg
    39421 gttacgccat ctgcaacagc tgggtcagca aattcagtct cctgaccttc ccgcctggga
    39481 agataacgtc ggtgaatgga ttgaagagat gaaagaagag tggcgtcacg acgtagaagt
    39541 actgaaagcg tgggtggcaa aagccactaa aaaatgaccc cggctagacc ggggtgcgcg
    39601 aatactgcgc caacaccagg gaaatcttgg ctgcgccgta aattattatg atgagttaca
    39661 agggcacagc actgtttttc aggccgcacg cagttaagat agcaaaactt aaatgatttg
    39721 ttacatgaat cagttaaatg tgtgatgcgt agcattttta ttaaaaaaat taatgtgttt
    39781 cagtcaattg cgaaaggatg atcacaatga agaaaattgg cgtaattctg agcggatgcg
    39841 gcgtctatga cggttctgaa attcatgaag cggtgttgac gttgttagct atttcacgca
    39901 gcggtgcgca ggcggtctgc tttgcaccgg ataagcagca ggttgatgtt atcaaccatt
    39961 taacgggcga agcgatgacg gaaacgcgca atgtgctgat tgaagcggca cgaataacgc
    40021 gcggtgaaat ccgtcctctg gcccaggccg atgccgctga actggatgcg ttgattgtgc
    40081 cggggggttt tggcgcggcg aagaatttaa gcaattttgc cagtcttggt agcgaatgca
    40141 ccgttgaccg tgaattaaag gcgctggcac aagcgatgca tcaggccgga aaaccgcttg
    40201 gttttatgtg tattgccccg gcgatgctgc cgaaaatttt cgatttcccg ctgcgtttga
    40261 ccatcggtac tgatatcgat accgcagaag tgctggaaga gatgggcgcg gagcatgtgc
    40321 cgtgtcctgt cgatgatatc gtggttgatg aagacaataa gattgtcacc accccggcat
    40381 atatgctggc gcagaacatt gcagaagcgg cgagcggcat tgataagctg gtttcccgcg
    40441 tgctggtcct ggcggaatga gtaaaagccg cttaacggtg tttagtttca ttcgccgttt
    40501 tcttctgcgg ttaatggttg tcctcgccgt tttctggggt gggggcattg cgttgtttag
    40561 cgttgcgcct gttcctttct ctgcggtaat ggtcgagcgg caagtcagcg cctggctgca
    40621 tggcaatttt cgttacgtgg cacattctga ctgggtcaac atggatcaaa tctcgccgtg
    40681 gatgggactg gcggtgattg ccgcagaaga tcagaagttt cctgagcact ggggctttga
    40741 tgtcacttcc attgagcaag ccctggcgca caacgaacgc aatgaaaacc gtattcgcgg
    40801 tgcatcaacg atttctcagc agacggccaa aaatctcttt ttatgggatg ggcgtagctg
    40861 ggttcgaaaa gggctggaag ccggattaac gctggggata gaaacggtct ggagcaaaaa
    40921 gcgtatcctg acggtttacc tgaatatcgc cgaatttggc gacggtgtgt ttggcgtcga
    40981 agctgcggca caacgttatt tccacaaacc cgcgagcaag cttacccggt cggaagctgc
    41041 attactggca gctgtattac ctaatccact tcgtttcaaa gtctccgcgc catcgggcta
    41101 cgtgcgtagc cgtcaggcgt ggattttacg gcagatgtat cagttaggcg gtgagtcgtt
    41161 tatgcaacaa caccagctgg attaagcttc gcgcgaagtg agggtcagcg ttttgctttc
    41221 acgaggttgc agatctttct gcagggcaat agtcagcgcc ggttgggcga taaatcgttt
    41281 ccataaccgc tcttgcttac gcagacaaca ccacttcgac caggtcgcca gtggaatcaa
    41341 tgtgctttca ccaatgttgt cgcagacgac ggggataacc tctgactcac tgatgcggtg
    41401 acagaggata ttctgcggct tcagcgacat cgtcacgata cggttatcct gcaaataacg
    41461 cttcagctgt ttcagtagct ggcgcagttg tgcaatgtct tcttcgtaac ggcattgtgc
    41521 agcaaattcg gtcagcgtaa tggagggttt gccgtcaaaa tctgcaatca catcgtagac
    41581 ataaccggtt ccgcaatccg tctcaacggt accgtgatag cgcggtattc cactccagtc
    41641 ttttaaccgg cgaccaagat gcgcgtagta ctttaactca cggcggatct ctttatcgcc
    41701 accatcgcca cgatggtaga caatcttgat acagcgttgg gcatcttccg gatgcgcata
    41761 acatttccga tgtcttccgg tgcccagggg actttgttca gataagcgaa tcatgccatc
    41821 tcctggaaat attttctgac gatctccagt atggtcgatt aacttaaacg aaacctaaac
    41881 aatgcttctt aaggttttct taatgttctt ctgaaagtga aaagcgcacg aaatgtacaa
    41941 atgacgttga ttgagaacga tttcagtacg cgcttttctt attgtcgggg gagcttgaag
    42001 ggatttgaat gtcaaagtga tgaagattaa tcttcatcaa aaccagaatt aaagagggcg
    42061 ataacggcgg ccagtgcttc ctcttcctgt ggaccggtcg cttcaacttc aatctgccgt
    42121 cctttggcag aatccagcat cagcagtgca ataacgctgt tggcttcggc ctcggtacct
    42181 tcgtcattac gtaacagcac ttctgcgtca aaaccttgca ttaattcaaa cagcttcatt
    42241 gcaggccggg catgcatgcc cagcttgttt gtgatttcaa cagtttgctt gacggtcatg
    42301 gtttacgttt ttccagcgta cgatggcgtg actggacgtt tttaccgcgc gagcggaagt
    42361 agtctgccag ttgctctgca atatacaccg aacggtgctt cccgccggta caaccaatgg
    42421 cgaccgtcaa gtagctacgg ttgttggttt ccagcatagg taaccatagc tcaagatagc
    42481 ttcgcgtctg gtagataaaa ttgtgtactt ctgtgtggcg gtcgaggaac gcggcgacag
    42541 gtttatcaag acctgtcatt ggacgcagtt tcggatccca gtgcgggttc ggcaagaagc
    42601 gcacgtcaaa gacgtaatct gcatcgatag ggataccgtg tttgaagccg aaagactcaa
    42661 agaccatggt cagttcgcgt tcacgtttac ccagcagacg ggtacgcagc atttcagcca
    42721 gctcgtgaac ggacatttct gaggtatcga caatcagatc cgctcgcgaa cgcagaggct
    42781 ccagcaaatc gctttctttg tcgatagcac tttccagcga caggtttttg ctggaaagcg
    42841 gatgcagtcg gcgcgtgtca ctgtaacgac gaattaaggt attacggtcg gcatccagga
    42901 acagtagttg cggtgagaaa gcgtcaggca ggttactcat cgcctgttcg aatatttctg
    42961 gtgactccgg catattacga acatcaatgc tgacggcggc agaaatctcg cgatcggcca
    43021 gggttcgagc cagatcgggt aacaacacta cgggaaggtt atccacgcag taaaaaccca
    43081 tatcttccag cgcacgcagg gcgacagatt tacctgaacc tgaacgtccg ctgacgatca
    43141 tcagtaccat gtactgtttc tcctcacaac gtctaaaaga gacattaccg aataactacg
    43201 cttcatccgg agtaccttcg gtatccgtaa tgatttgata cagctcttca tcgctctggg
    43261 ctgcgcgtaa acggcggcag atggttttgt ccgccagacg tttcgccacc agcgacagag
    43321 tatgcaggtg cgttttagtt tggtctgccg gcaccagcag ggcaaaaagt aagtccaccg
    43381 gttggttgtc gatggcatcg aaagctatag gcgtttcgag ctgaacgaaa acgccaacgg
    43441 cgcgcagagt atcttcttcc agtttgccat gcggaatggc aataccattg ccgataccgg
    43501 tactgcccat tttttcccgc gtcaggatag cttcaaaaac cacctgaggc ggcagactaa
    43561 gttgtttcgc cgccagctcg ctgatgattt ccagggcgcg ttttttgctc tgacagtgga
    43621 cgcggcttcg cgtacattcc ctgttaagaa cactgctaag ctgtagagtt gtatcattat
    43681 ttgtcataat ttcacctaag aacctgccca ctcaaacagt ggaacaggct cggagcatta
    43741 accgtacaaa tggcccgttg tgccgcacaa caggccgcca tgcacatgct aattgcccgg
    43801 acaattagtg ttgtttcagt ttatctttat gtttggtcaa ctggcgtgcc agcttatcaa
    43861 ttaagccatc aatggcagcg tacatgtcct gaccttccgc gctggcatga atttcaccac
    43921 cgtttacatg cagtgttgca tctgaggtgt gggtgacttt ctccactttc agaacaacat
    43981 agacctggtt gattcggtca aaatattgct caagtttggc aaatttggct gtaacaaatt
    44041 cgcgcagtgc ctcggtgatc tcgacgttat ttccggtaat gttgagctgc atagtgtctt
    44101 ccttatcggt tgggtcaaac gagctgttta cgctggtttg acggcggaat ggataaagac
    44161 tctcggtact tcgcaacagt gcggcgtgcc accatgatac cttgttccga cagcaaagag
    44221 gttaacttgc tgtcgctcaa cggtttcgct gggttttccg ccgcgattaa tttcttaacc
    44281 agcgcacgaa tcgccgtgga ggaagcttcg ccgccgccct cggtattgac gtgactggaa
    44341 aagaaatact tcagttcaaa aatgcctcgt ggactatgca ggtatttttg cgtggtcacg
    44401 cgagatatcg tcgattcatg catttcgaca gcctgggcga tatcggccag taccatcggt
    44461 ttcatatatt cttcgccttg ctcaaagaag gcttgctgct gttcaacgat acagcgactc
    44521 acgcgcagta gcgtatcgtt acggctttcc agactcttga tcaaccattt ggcatcctgc
    44581 agattgctgc ggataaactg gctgtcacca tcgttgcgcg cgttattgca catcgaggcg
    44641 tagtgctggt tgatttgcag acgcggaatg ctgtcactgt tgagttctac cgtccagtga
    44701 ccgttatgct tacgcaccag cacatctgga atgacatact caggttcgcc cgtctggatc
    44761 gactgcccgg ggcgcggatc gagcgactgg atcagattga cggcttcttt cagcacatct
    44821 tctttcagac gcgtgacgcg cattaaagtg cggaagtcgt gattggctaa cagatcgaga
    44881 tgatcgctaa tgatcagtct ggcctcttcc agccatggcg tggttttatc gaattgggag
    44941 agttggatca gcagacagtc acgcagatct tttgctgcca caccgaccgg atcaaaccgt
    45001 tggatccgct taaggacggc ttcaacttcg tcgatgtcaa tctcttcatc gcccatactt
    45061 tcgagaatat cttccagtgg gacagtcaga taaccggtgt catcaacggc atcgacgata
    45121 gaggtagcaa tagcgcggtc agtgtcagaa aacggtgtca actcgacctg ccacatcagg
    45181 taatcctgca aggtctgcgt cgtttcgccc tgatagaccg gcagctcgtc gtcaatgtag
    45241 tcaccgctgg tgccggatgg tgtaccagcg gtgtaaatgg tgtcccaact ggcatcgagc
    45301 ggcagctctt ccggcatctc tttttgttcg agcgcgtcgg cggtgtccag cgtttcactg
    45361 tcttgcgttt cgcgggtgtc gatttcttca tgagtgtcga tttgctcaag cagcggatta
    45421 ctctccagcg cctgctgtag ctcctgctga agttccagcg tcgacaactg caacagacga
    45481 attgcctgtt ggagctgtgg cgtcatcgcc agttgttggc taagcctgag ttgcaaacct
    45541 tgcttcatgt tcagaatcgt actctcctgc taaaacgtcg caaacttcta ccctatcaga
    45601 gtctgaagtc ttccccaagg tatacacgct taacgtgttc gtcttgtaag atttccgtag
    45661 gcgtgccgtg ggcgatcaaa tgcccctgac tgacgatata agcgcgttca caaaccgcca
    45721 gtgtttcacg cacgttgtgg tcagtgatca gtacgcccag gccgctgtcg cgcaggtgct
    45781 caatgatgcg tttaatgtcg ataaccgaga tcgggtcaac cccggcaaac ggttcgtcga
    45841 gcagaataaa tttcggattc gcagccagtg cgcgggcaat ttctacacgg cgacgttcac
    45901 ccccggagag tgactgcccc atgctgtcac gcaggtgctc aatgtgaaac tcttccatca
    45961 gctcgttcgc gcggtcttca cgttgttcag cagacaagtc gtcacgaatt tgcagtaccg
    46021 ccatcaggtt atcgtaaacg ctgaggcgac ggaaaatgga ggcttcctgt ggcagatagc
    46081 cgataccgcg gcgcgcgcgt gcatgcagag gcagcagact gatatcgtca tcatcaataa
    46141 tgatgttgcc cgcatcgcgc ggcacaatgc ctacaaccat gtagaaagtg gtggtcttac
    46201 cggcaccgtt tggccccagc agaccgacaa tttccccgga gttgacggtc aggctgacgt
    46261 cttctaccac gcgacggcct ttataggctt ttgcaaggtt ctttgcagtt aatgttgcca
    46321 taacgaatta attacccttc ttctgtgccg gggtctggcc tttgttgttt ttgtcctgca
    46381 gctgcgacgg caccagaacg gtggttacgc gcttaccttt gtcgctgaaa gcctgcattt
    46441 tctgctcttt caccaggtaa gtgatcttat cgcccttaat gttgctatcg acctgctgca
    46501 gataagcatt acccgtcaga acgacaaaat cttttgccag ttcgtagtgc atctgggaag
    46561 cgtgaccttc aacgggttta ccattgtcct gcatctggta gaacgttgcc ggtttaccgt
    46621 agccgtcaat cacttcttta ccttgttcgc cgcccggacg ggtaacgacc actttgtcgg
    46681 cattaatttt gatggtgccc tgggtgacga tgacattacc ggtaaaggta accacgttgc
    46741 cttgcatatc aagagattgc tggtccgatt caatgtggat cggctgatca gtgtctccgg
    46801 ttacggcaaa tgccggaatg ctggcggcca gaagtgagct ggcaagcaca agattaaggc
    46861 tgagtttgtt tgttttgaat ttcataggat gttctaacct tttcaatcag ctcggcgttc
    46921 ttgctgcgta agttgccgcg cattttcaga ccgctggagt taaatgttgt tccgtataac
    46981 gtgacgaggt cttcagaggt aacatcctgc gtcaccagat tgatctgcgc gttatccgtc
    47041 gtgattctgc gaagttgaga gtccggcaca agtgcgttga cttcaacgtg tccatataaa
    47101 tagagcatcc ggtcattggt cagcttggct ttatctgctt ttacggacca tgtcgggatt
    47161 ttatccttat caaacgtggt aagtaccggc tgcgtaaacc acgaaacggc ctgatcggaa
    47221 taatattcaa catgttgagc aatcaatcga tagcttagtg ccccttctgg gttatagacg
    47281 agcgtgtccg tatgctcgct tttataggtg ggatcattgt tgttgacgac cacctgggcg
    47341 gtatcgtctt tttcggccat attaatgccg atcatcacca gaaccgccag tgatagcaca
    47401 atgataaccc aacgtctggc tttactcata tcgattgccc tttggcttca tccagtttgc
    47461 cctgcgccag gagtaataag tcgcaaactt cacgcactgc accacgaccg ccagcaatgc
    47521 gcgtcacgta atcggcgcgc gggagcaaca gtggatgcgc atcggccacg gcgacgctta
    47581 aacccacttt ttccattacc ggccagtcga tgagatcatc gccgacataa gccacatttt
    47641 ccggggcaat cgccagtttt tccagcaaat cgctaaaggc gatcagtttg tttgactgcc
    47701 cctgatacaa gtgagtgatc cccaatgtgg cacaacgatc ttctaccagt ttagcctttc
    47761 gcccggtaat gatagcgact tcaatatcag aggtgagcgc acaacgaatg ccataaccgt
    47821 cacgaacatt gaacgctttc agctcttcgc cattattgcc catataaatc aggccatctg
    47881 acagtacgcc atcgacatcg aggatcagca gacgaatgtt ctctgctttt gctataacgt
    47941 cggcgctgac agggccgtaa caggtcgcaa gcgacgcacc tgctttgctc attgttgttt
    48001 atccttgaat ctttacacta cgcctgcacg cagtaaatca tgcatatgta acacaccgag
    48061 taaatggtcg ccatcggcaa ccatcacgga ggtgatatgg cgggactgca ttaagttcag
    48121 tgcctcaacg gccagaatgc cagggcgcac acgtattccc cccggcgtca tcacatcggc
    48181 aatacttaac tgacgaacat ccacgcccat atcgaagaca cggcgtaaat caccgtcggt
    48241 aaagatgcct tcaatcatca tattgtcatc gcaaatgaca gtcataccaa gatttttgcg
    48301 ggtaacttcc agcaatgcgt cacgcagact ggccgttttc ttaacatgcg ggatctcatc
    48361 gcccgtatgc ataatatcgt ttacgcgcag cagaagttta cgacccagtg cgccgcctgg
    48421 atgtgagagc gcaaaatctt cagcagtaaa gccgcgtgct tttaacagcg cgacagcgag
    48481 ggcatcgccc ataaccagcg tggcggtggt gctgctggtc ggtgccagcc ctaacggaca
    48541 ggcttctttc gctactttaa cacacagatg cacatctgcg gcgcgcgcca tgctgctctc
    48601 cgggcgaccg gtgatgcaga ttaacggtac gtgaagacgc ttaagcactg gaattaaggc
    48661 cgtgatttcg ctggattcac ctgagttaga gatagcaatc accacatcct gtggggtgac
    48721 catgcctaaa tcaccatgcg cggcttcacc aggatggacg aaaaatgaag gtgtaccggt
    48781 gctggcaaac gttgccgcca ttttgcgccc aatatgcccc gatttcccca tccccatgac
    48841 gacaactttc cctttacacc agaacatctt ttcacaggca agcgtgaaat tctgattgat
    48901 gtattgatca agctccgcca ggcattcacg ttcaatcgcc aggacttctt tacctgcttg
    48961 ctgaaagtca aaacccggtt gtaactctac gtgcgacata atgcgtttcc agttattcaa
    49021 cgagtattgg cgataaccag tacaacatcg ccagccatac gataaatccg cctgttaata
    49081 atacccctac accacggccc gattgcgggg agcgccgcca gcacagcaac gcaaaaataa
    49141 tgctcaccag caacatcacg ctgtagtcac gactgtacgc cagtggatca atctctcctg
    49201 gcgttatcag cgcgggtaaa cccaacacga tgacaatatt aaaaatgttt gcgccaatga
    49261 tatttccgac agcaatgtcg ttttcacctt tgcgaacccc cgctattgcg gttgccagtt
    49321 ccggcaggct ggttccgata gcaattgccg tcagacccat cgtcaactcg ctgatggcaa
    49381 agtaattcgc cagcaccgtg gcgttatcaa ccaccatccg cgtggccact ggcatgatga
    49441 taagcgcaat gccgagccat aaaaacgcga cgggcaatcc gccgtcacga ggcagctctg
    49501 caagctgctc tctggtcagg ctgtcagtcc cctgacgttc agcctgacgt gcaagtttaa
    49561 caatgaacag cagccatagc acagccagaa agaggagaaa gataccatcg ctgcgactaa
    49621 gttgtccgtc atagagtacg gaaccggcca ccacgctgac caacaacatt aagggtaatt
    49681 cacggcgtag aacatcagaa tggacggtaa aaggacgaac cagcgcagcc agaccgagga
    49741 tcagcaatat attgataatg tttgagccga gggctgtacc aacggctaaa tcgcgttgtt
    49801 cgtgcagaga cgcggcaagc gagacgatga tttctggtaa cgatgtacca atactgacca
    49861 ccgtcatgcc gatgatcagc ggcgggatgc caaaggttcg gcaaagaata gacgcggcaa
    49921 aaaccaggcg gtcggcactg taaacgacca aaagtaaacc aacaattaac agtgccgtag
    49981 ctaaaagcat ctaacgtcct ttcttcaggt atactcgccg gtccgctgaa gattttcaga
    50041 aagccgtaac ggattcttaa ttttgacttt atgcggctaa aaagtaaaac aaatgccagc
    50101 tttcgctaac cacggcgggt aatattctgt aaatatgttg ggttcaaggt taaattgagc
    50161 gccatgctta gaaaatcaac gcaagacgaa gggtgaatta tggagcagtc tgtggcgaat
    50221 ttagtcgata tgcgcgatgt cagttttacg cgtggcaatc gctgcatctt cgataatatt
    50281 tccctgaccg tgccgcgagg gaagatcacg gcgatcatgg ggccatcggg catcggtaaa
    50341 acgacgctac tccgtctgat tggcgggcaa atcgcaccag atcatggtga gattcttttc
    50401 gatggtgaga atattccggc gatgtctcgt tcgcgcctgt atacagtgcg caaacggatg
    50461 agcatgttat ttcagtccgg ggcgttgttc actgatatga acgtatttga caacgtcgcc
    50521 tatccactgc gcgaacatac tcaacttccc gcgccattgt tgcatagtac ggtgatgatg
    50581 aagctggagg ccgtggggct gcgtggagcg gctaaactaa tgccttctga actttccggt
    50641 gggatggcgc ggcgtgcagc gctggcgcgt gcgattgcgc tggagccgga tctcatcatg
    50701 tttgatgaac cctttgttgg gcaagatccc atcaccatgg gcgtactggt gaagctgatt
    50761 tctgagctga acagcgcgct gggtgtgact tgtgtggtgg tttctcacga tgtgccggaa
    50821 gtgttaagta ttgccgatca cgcctggatc ctggcggaca aaaaaattgt cgctcatggc
    50881 agtgcccagg cgttgcaggc gaatcctgat ccacgcgtac gtcagtttct ggacgggata
    50941 gctgacgggc ctgttccgtt ccgctatcct gccggcgatt atcacgctga tcttttacca
    51001 gggagttaag ccactcatgc tgttaaatgc gctggcgtcg ctcggacata aagggattaa
    51061 aaccctgaga acgttcgggc gggccgggtt aatgttattc aatgcgctgg tcggcaaacc
    51121 ggaatttcgc aaacatgcgc cgctgctggt gcgccagctc tataatgtcg gcgtcctgtc
    51181 gatgctgatt attgtagttt ctggcgtgtt catcggaatg gtgttggggc tgcaaggtta
    51241 tctggttctg accacttata gtgcggaaac cagtctgggt atgctggtgg cgttatcgct
    51301 actgcgtgaa ctggggccgg tggttgccgc gttgttgttt gccgggcgtg ctggttcggc
    51361 gctaaccgca gaaatcggcc tgatgcgcgc tacagagcaa ctctccagta tggagatgat
    51421 ggcggtggat ccgctgcgtc gggttatttc tccccgtttc tgggctgggg ttatttcatt
    51481 accactgttg acggttattt tcgtcgccgt ggggatctgg ggcggatctc tggtcggcgt
    51541 cagttggaaa ggcattgata gcgggttctt ctggtcggca atgcaaaatg ccgtcgactg
    51601 gcgtatggat ctggtcaact gtctgattaa gagcgtggtg ttcgccatca cggtgacgtg
    51661 gatttcgttg tttaacggct acgacgccat cccgacgtct gccgggatta gccgggcaac
    51721 cactcgcacc gttgtccact cgtctctggc tgttctgggg ctggattttg tgctgaccgc
    51781 attgatgttt gggaattgag ttcatgcaaa cgaaaaaaaa tgaaatttgg gtgggtatct
    51841 ttttattagc agcactactg gcggcgctgt ttgtttgcct gaaggcggcg aacgtgacgt
    51901 ccatacgtac tgaaccgacc tacacgcttt atgcgacgtt cgataacatt ggcggcctga
    51961 aagcgcgctc tccggtcagc attggtggtg ttgttgtggg ccgggtggcg gatattacgc
    52021 tggacccgaa aacctatctg ccgcgcgtaa cgctggaaat tgaacaacgt tataaccaca
    52081 ttcctgatac cagttcgctg agcattcgta cttccggcct gctgggggaa caatatctgg
    52141 cattaaacgt cggttttgaa gacccggaac tggggactgc tatcctgaag gatggcgata
    52201 caattcagga caccaagtct gcgatggtgc tggaagatct tattggtcag ttcctttacg
    52261 gtagtaaagg cgatgacaat aagaatagtg gcgatgcgcc agctgctgcg ccaggtaata
    52321 atgaaaccac tgaacctgtg ggtacaacga aataatttca ggagaaccga cgcatgttta
    52381 aacgtttaat gatggtcgct ttgctggtga ttgcaccgct gagtgcggca accgcggcag
    52441 accagaccaa tccgtataag ctgatggacg aggcggcgca gaaaacgttc gatcgcctga
    52501 agaatgagca accgcaaatt cgggccaacc cggattatct gcgtaccatt gtcgatcagg
    52561 aactgctgcc atacgtacag gtgaaatacg ccggtgcgct ggtgctgggc cagtattaca
    52621 agagtgcgac cccagctcaa cgtgaagcct actttgccgc tttccgtgag tacctgaagc
    52681 aggcttacgg tcaggcgctg gcgatgtatc acggtcaaac ctatcagatt gcgccagaac
    52741 agccgctggg tgataaaacc attgtgccta ttcgcgttac cattattgac ccgaatggtc
    52801 gcccgccggt gcgtctggac ttccagtggc gtaaaaactc ccagacgggc aactggcagg
    52861 cttacgacat gattgctgaa ggcgtcagta tgatcaccac caaacaaaac gagtggggaa
    52921 cgctgctgcg taccaaaggt atcgacggcc tgactgcgca actgaaatcg atttctcaac
    52981 agaaaatcac tctggaagag aaaaaataat gagcgagtca ctgagctgga tgcagacggg
    53041 tgacacgctg gcgctatccg gagagctgga tcaggacgtt ctgctaccgc tttgggaaat
    53101 gcgtgaggaa gcggtgaagg ggattacctg catcgatctt agccgtgtgt cccgcgtgga
    53161 tacgggtgga ctggcactgc tgctccatct tatcgatctg gcgaaaaagc agggcaacaa
    53221 cgtgacgctt cagggggtaa acgataaagt gtataccctg gcgaaattgt ataatttgcc
    53281 tgctgatgtt ctgcctcgtt aaattttttc aggccggata tggcgttaac cgcctgtccg
    53341 gcaaacctgc ctgcaagtcg agccttgtct gatttatcag cgaggctttt tgcttattta
    53401 tgccagcgca actttgctct aagatgtttc gctggtttat taactgatga ttgaagatcc
    53461 catggaaaat aatgaaattc agagcgtgtt gatgaacgct ctctccctcc aggaagtcca
    53521 cgtttccggc gatggcagcc actttcaggt tattgccgtg ggtgagttgt ttgacggcat
    53581 gagtcgggtt aaaaaacagc agacggtcta tggcccgctg atggaatata ttgcggataa
    53641 ccgcattcat gctgtgtcga tcaaagcgta tacccctgcg gagtgggcgc gcgatcgcaa
    53701 actgaacggc ttttgagcta tgggcgattc gccggtagcg gatatgaatt gttaactgag
    53761 aacaaactaa atggataaat ttcgtgttca ggggccaacg aagctccagg gcgaagtcac
    53821 aatttccggc gctaaaaatg ctgctctgcc tatccttttt gccgcactac tggcggaaga
    53881 accggtagag atccagaacg tcccgaaact gaaagacgtc gatacatcaa tgaagctgct
    53941 aagccagctg ggtgcgaaag tagaacgtaa tggttctgtg catattgatg cccgcgacgt
    54001 taatgtattc tgcgcacctt acgatctggt taaaaccatg cgtgcttcta tctgggcact
    54061 ggggccgctg gtagcgcgct ttggtcaggg gcaagtttca ctgcctggcg gttgtacgat
    54121 cggtgcacgt ccggttgatc tacacatttc tggcctcgaa caattaggcg cgaccatcaa
    54181 actggaagaa ggttacgtta aagcttccgt cgatggtcgt ctgaaaggcg cacatatcgt
    54241 gatggataaa gtcagcgttg gcgcaacggt gaccatcatg tgtgctgcaa ccctggcgga
    54301 aggcaccacg attattgaaa acgcagcgcg tgaaccggaa atcgtcgata ctgcgaactt
    54361 cctgattacg ctgggtgcga aaattagcgg tcagggcacc gatcgtatcg tcatcgaagg
    54421 tgtggaacgt ttaggcggcg gtgtctatcg cgtgctaccg gatcgtatcg aaaccggtac
    54481 tttcctggtg gcggcggcga tctctcgcgg caaaattatc tgccgtaacg cgcagccaga
    54541 tactctcgac gccgtgctgg cgaaactgcg tgacgctgga gcagacatcg aagtcggcga
    54601 agactggatt agcctggata tgcatggcaa acgtccgaag gctgttaacg tacgtaccgc
    54661 gccgcatccg gcattcccga ccgatatgca ggcccagttc acgctgttga acctggtggc
    54721 agaagggacc ggcttcatca ccgaaacggt ctttgaaaac cgctttatgc atgtgccaga
    54781 gctgagccgt atgggcgcgc acgccgaaat cgaaagcaat accgttattt gtcacggtgt
    54841 tgaaaaactt tctggcgcac aggttatggc aaccgatctg cgtgcatcag caagcctggt
    54901 gctggctggc tgtattgcgg aagggacgac ggtagttgat cgtatttatc acatcgatcg
    54961 tggctacgaa cgcattgaag acaaactgcg cgctttaggt gcaaatattg agcgtgtgaa
    55021 aggcgagtaa ttgtctgaga gctggcagcc ccgttcgcgg ggctaccgcc agaccactca
    55081 ttttttcggt ttagtgtagc tacgcatcaa ctgtgttctg tcgataaact catgggtctg
    55141 cggatcatgg tagcgagatg gccagataat ccaggggtca gttcccaggg ctttcgcaat
    55201 aatcatctct cctttcggcc aggggcgcga taatgcgttc gccagcgttg aggaactcaa
    55261 accatttctg cgagattccg ccgccattga cgttcccttc ttgcgcaaac ccgcaatgat
    55321 gtcagcggga tgccagtcaa tgaaattact ttccatcttt cctcactcct taagttagtc
    55381 gctttcattc agaacacttc ttctgtcgag catcactata ctcctgatgg cctattgctc
    55441 taaaaagttc ctgtaaagag tgtgaatatt cagaatatat aaagatgtac agccctattt
    55501 ttcaggtgct atagagctga ataagtgttt ttatggaact tattggaatc atcccacgca
    55561 ctgcgcggga tgcggggagg ggattaacga tcgcgttgaa cagcgatgtg cgcgaggccg
    55621 atgagtgctt ctcgccacgg ggtgtccggg agcacctgta acgctgcgat ggctttgtct
    55681 gcttcttcct cggcacgctg acgcgtccat tcaagagatc cacaagcgtt cattgcttcc
    55741 agaaccggtt ccagaagatg gcgaccgtta ccctgttcga tggcggtacg aatcatctgt
    55801 gcctgttctg gtgtgccatg atgcattgca tgcagcagcg gcagcgtcgg tttaccttcg
    55861 ttcagatcgt cgccgacgtt tttacctaac tgttcgccat cggcattgta atcgagtaaa
    55921 tcgtcgatca actggaaagc agtgccgaga tagcgcccat aatcctgcag gcctttctcc
    55981 tcctccggcg tacagccagc cagaataccg gaacactgcg cggcagcctc aaacagacgc
    56041 gcggttttgc tatagataac gcgcatgtag ttttcttcag tgatgtccgg atcgttaacg
    56101 ttcatcagtt gcagaacttc accttctgcg atgacgttta cggcttctga catgacttcc
    56161 agcactttga gcgagccgag gctggtcatc atctggaacg cgcgggtata aataaaatcg
    56221 cctaccagca cgctggcggc attgccaaat gcggcgttgg cggtagcttt acccctgcgc
    56281 atatctgatt catccacaac gtcgtcgtgc agcagagtcg ccgtgtggat aaactcgatc
    56341 agggcagcaa tggtgacatg cgcatttccc tcatagccaa cagctcgtgc agccagtaca
    56401 gcaatcatcg gacgaatacg tttaccgccg ccgctgacga tgtaatagcc taactgattg
    56461 atcagttgga cgtcggaatt aagctgctca aggattgccg cattaacacc cgccatatct
    56521 tgcgcggtta actcattgat tttttctaaa ttcatcgcaa aagccgggct tttcgccccg
    56581 attaccttac tgtgaacaaa ggcaacttag ggtttatggt taggaatata gggtgattgt
    56641 actgaaaaaa tggcacagat aaacgttacc gtacaagttg tgtttttttt cttcgtgtat
    56701 tgactgtagc acttgtcaaa ggcgtgcgtt ttgcgtaata ttcgcgccct attgtgaata
    56761 tttatagcgc actctgaatc attgaaaagg tgtgcgcgga agcggagttt tatatgtacg
    56821 cggttttcca aagtggtggt aaacaacacc gagtaagcga aggtcagacc gttcgcctgg
    56881 aaaagctgga catcgcaact ggcgaaactg ttgagttcgc tgaagtgctg atgatcgcaa
    56941 acggtgaaga agtcaaaatc ggcgttcctt tcgttgatgg cggcgtaatc aaagctgaag
    57001 ttgttgctca cggtcgtggc gagaaagtta aaatcgttaa gtttcgtcgt cgtaaacact
    57061 atcgtaagca gcagggccat cgtcagtggt tcactgatgt gaaaattact ggcatcagcg
    57121 cctaagacct gaggagagat ttcaaatggc acataaaaag gctggcggct ccacacgtaa
    57181 cggtcgcgat tcagaagcta aacgcctggg cgttaagcgt ttcggtggcg aatccgttct
    57241 ggcgggtagc atcatcgttc gtcaacgtgg taccaaattc cacgctggcg ctaacgtagg
    57301 ttgcggtcgt gaccacactc tgtttgctaa agcagacggt aaagtgaaat tcgaagttaa
    57361 aggcccgaaa aaccgtaaat ttatcagcat cgaagctgaa taagtttttc gcgt
//
LOCUS       DAPOZO010000022        56591 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPOZO010000022 DAPOZO010000000
VERSION     DAPOZO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.022"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            39..1619
                     /locus_tag="ROI21_001429"
     CDS             39..1619
                     /locus_tag="ROI21_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDX5110372.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPAYDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKSAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIR"
     gene            1619..2803
                     /locus_tag="ROI21_001430"
     CDS             1619..2803
                     /locus_tag="ROI21_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDX5110373.1"
                     /translation="MNVFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRALKKRLEHPILGYSRWQHDDYLNAIVNWYHQQYKTEIKPEWITYSPSVMYSIAK
                     AIELLTLNGDNILIFTPVYNAFFDVIKQSGRNMLTTSLNKENERGYAINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEEDLFKIAASCAKQKIWLCSDEIHSDFVFNHGFTSALKI
                     KNDKVVVLNSISKTFNVPALTGSYMISADQDFNDKFRTISRYRDFVNSPSILNVIATI
                     VAYNECEEWLKSLKDHIASNIQFTQQYLNENIPELIVRSAEGCYFSWIDCSAIGYPFD
                     KFYRRLIDEGKVGIMAGYVYGTEGEGYLRLNLACGREKLYMGLVRLASVIKNINQEKE
                     NE"
     gene            2796..3392
                     /locus_tag="ROI21_001431"
     CDS             2796..3392
                     /locus_tag="ROI21_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDX5110374.1"
                     /translation="MNNSELIHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKRMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            3464..4411
                     /locus_tag="ROI21_001432"
     CDS             3464..4411
                     /locus_tag="ROI21_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDX5110375.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVKNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWVESHGCELQGKRIGLVGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDFQAAGKDGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(4490..4566)
                     /locus_tag="ROI21_001433"
     tRNA            complement(4490..4566)
                     /locus_tag="ROI21_001433"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4530..4532),aa:Met,seq:cat)
     gene            complement(4601..4677)
                     /locus_tag="ROI21_001434"
     tRNA            complement(4601..4677)
                     /locus_tag="ROI21_001434"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4641..4643),aa:Met,seq:cat)
     gene            complement(4711..4787)
                     /locus_tag="ROI21_001435"
     tRNA            complement(4711..4787)
                     /locus_tag="ROI21_001435"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4751..4753),aa:Met,seq:cat)
     gene            4996..6093
                     /gene="mltA"
                     /locus_tag="ROI21_001436"
     CDS             4996..6093
                     /gene="mltA"
                     /locus_tag="ROI21_001436"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDX5110376.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            6169..6975
                     /gene="tcdA"
                     /locus_tag="ROI21_001437"
     CDS             6169..6975
                     /gene="tcdA"
                     /locus_tag="ROI21_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDX5110377.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(7026..7469)
                     /gene="csdE"
                     /locus_tag="ROI21_001438"
     CDS             complement(7026..7469)
                     /gene="csdE"
                     /locus_tag="ROI21_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDX5110378.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTMAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAVAKQV"
     gene            complement(7469..8674)
                     /gene="csdA"
                     /locus_tag="ROI21_001439"
     CDS             complement(7469..8674)
                     /gene="csdA"
                     /locus_tag="ROI21_001439"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDX5110379.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGSKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            8866..9093
                     /locus_tag="ROI21_001440"
     CDS             8866..9093
                     /locus_tag="ROI21_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDX5110380.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9444..10361
                     /gene="gcvA"
                     /locus_tag="ROI21_001441"
     CDS             9444..10361
                     /gene="gcvA"
                     /locus_tag="ROI21_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDX5110381.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            10380..10775
                     /locus_tag="ROI21_001442"
     CDS             10380..10775
                     /locus_tag="ROI21_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDX5110382.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            10768..11868
                     /gene="rlmM"
                     /locus_tag="ROI21_001443"
     CDS             10768..11868
                     /gene="rlmM"
                     /locus_tag="ROI21_001443"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDX5110383.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(11912..12643)
                     /gene="fucR"
                     /locus_tag="ROI21_001444"
     CDS             complement(11912..12643)
                     /gene="fucR"
                     /locus_tag="ROI21_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDX5110384.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12701..13123)
                     /gene="fucU"
                     /locus_tag="ROI21_001445"
     CDS             complement(12701..13123)
                     /gene="fucU"
                     /locus_tag="ROI21_001445"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDX5110385.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(13125..14543)
                     /gene="fucK"
                     /locus_tag="ROI21_001446"
     CDS             complement(13125..14543)
                     /gene="fucK"
                     /locus_tag="ROI21_001446"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDX5110386.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFSTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSTQVDT
                     SLLSQYTGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQHNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(14652..16427)
                     /gene="fucI"
                     /locus_tag="ROI21_001447"
     CDS             complement(14652..16427)
                     /gene="fucI"
                     /locus_tag="ROI21_001447"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDX5110387.1"
                     /translation="MKINTLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLVMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            16897..18396
                     /locus_tag="ROI21_001448"
     CDS             16897..18396
                     /locus_tag="ROI21_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDX5110388.1"
                     /translation="MSETFLQMKHITKRFPGVLALNDVQFTLRRGEVHALLGENGAGK
                     STLMKILSGVYQPDEGEIIFEDKPVSFSDPLSAQNVGITIIHQEFNLFPELTVEENIF
                     IGREFCKKNRWRLDEKQQRQATIEILQKLNLAIKPDTLVADLTVAQQQMVEIAKAISV
                     NARILIMDEPTAALTETEIESLFRVTRLLKEQGTGIVYISHRLEELALIADRATVMRD
                     GQYISTVDYECVKISDLIAMMVGRDLGNIYPRREALQQRIPVLKVNGLTRKGVLNDIN
                     FTLYRGEILGFAGLMGAGRTELARAIFGADSIDSGTLKLNGKETVIKDISDAIQQGIS
                     YLTEDRKKEGLALNLSVERNIMLGNYPEYSDRFGNVDSRRCQQTSEEQVKALRIKTPN
                     LEQAALNLSGGNQQKIIIARWVCKDTDILIFDEPTRGIDVGAKLEIYELMNRLVAKGK
                     SIIMISSELPEVLGMCDRILVMRSGRITGELSAKEATQEKIMQYATLED"
     gene            18400..19383
                     /locus_tag="ROI21_001449"
     CDS             18400..19383
                     /locus_tag="ROI21_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5110389.1"
                     /translation="MTISVTSPDSNNKKIKINKELLMRLAPLFSLIILVLFFSFSSPF
                     FFNTENIMTIALQTSVIGIMAIGVTFVIITAGIDLSLGSVVAFSGVAVGICATLGLPL
                     PVCIIAGVLAGGLCGYVNGLLVTKMTIPPFIATLGLMMSVRGINMVMTDGRAIYFADY
                     PMFKTLAQGRLFDVLPYPVFYLVIVALVGAYILKKTVIGRYVYAVGSNEVAAHLSGIK
                     VQRVKIFVYAFCGLLTGIAGVILASRLNSGQPTVGVGYELEAIAAVVIGGTSLMGGIG
                     TIGGTIIGAFIMSVLKNGLNLMGVSQFWQMVAMGVVVVAAVYLDTLRKKIR"
     gene            19457..20407
                     /locus_tag="ROI21_001450"
     CDS             19457..20407
                     /locus_tag="ROI21_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDX5110390.1"
                     /translation="MKTRHFIYALSLLACVTSSALAKDLNLPVVSKGFQHEFWQTVKM
                     GTEAAAKELGDKTSYVGPADETQIAEQIQLVENAMAQKPNGLLLAALDANALAPLVET
                     ANSRGIKVVTFDSGINSDIPVSFVATNNRKAGAQAADALAAQVNNKGKVGIIAHVAGT
                     SSAIERSEGFMERMKEKYPDIKVLPVQYSDGDPQKAMDKTIDMIQANPDLAGIYGTNE
                     GSTLGVANAIDSQNLKGKVKVIGFDSTEAIINFLKNGVIQGFVVQDAYQIGYQGIKTL
                     NAALSGQAVEKEIDIPVKFVNAENINTPEIDKLLHPFGKK"
     gene            20461..22041
                     /locus_tag="ROI21_001451"
     CDS             20461..22041
                     /locus_tag="ROI21_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDX5110391.1"
                     /translation="MHNGYFLGVDVGSASVRAGVYSASGHRLAFATAPVSQFRPGGER
                     VEQSSAEIWQQVCKTVKEATALAGIPISAIRSLGFDATCSLVVLDEQGQGLAVSSGGS
                     ANHDIIMWMDHRATVETAQINATKDPALRYVGGEVSVEMELPKLRWLKTHLPQTWQAA
                     HRFFDLADFLVWKATGRDVAGLCTLTCKWNYLAHEQRFSHSLLESVDLTDMLERIPAE
                     ILPPGAAVGTLTATAAEELGLTTNVIVASGLIDAHAGGMALAGAQPSGTLALISGTSN
                     CHMLCSEKEIFTPGVWGPYWSAMLPNYWLTEGGQSAAGALVEWTLQESGASANLFNKA
                     QQRGCHPIQLINEWVAALEANESEPTRNLHVLADHHGNRSPRARSDARGSVYGLTLER
                     GENQLARLYLATLQAIAFGTRHIIETLRENGHTITRLTLCGGATHNPLWLREYADVTG
                     CDIHLMQEEDAVTLGAAISGAVASGAWGDFTSACKAMVEAGEVIQVNPQRREFLERKY
                     RVYLTLWEQQQAVNQLMQ"
     gene            22106..23104
                     /locus_tag="ROI21_001452"
     CDS             22106..23104
                     /locus_tag="ROI21_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDX5110392.1"
                     /translation="MAKTVEQIASDLNLSVTTVRLVLNGKAEQYRISVKTQTRINEYV
                     ERYGYIINHSARSLKLNKTDTLGLIVPNISNVFFATLAEKLEQRCRRSGYQLTISCTY
                     DDVDYENKLTKAFIARNVDGLFIVPSTLENQQHHLRKIRKPMVLLDRDFKYTDNALVE
                     SNNISGGEKLTQNILEAGQAPVWFLLGDAGLPSISDRLTGYLNALAKKGITHRDWVRE
                     GPVNTPEGGYVIMEKLIEEQGCPQAFIASSLPVLEGAVRAIRDRLGAVPPEINIGTFD
                     EHPMLGFLANNVWSMQQDENAWAEKAFEMMLSAIEERPVKKTVKVEMKLIKRIRKK"
     gene            23237..23884
                     /gene="fucA"
                     /locus_tag="ROI21_001453"
     CDS             23237..23884
                     /gene="fucA"
                     /locus_tag="ROI21_001453"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDX5110393.1"
                     /translation="MERTRLSREIIETCLEMTRLGLNQGTAGNVSTRFENGMLITPSG
                     IPYERMTENMIVYVDGNGKYEEGKIPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            23912..25060
                     /gene="fucO"
                     /locus_tag="ROI21_001454"
     CDS             23912..25060
                     /gene="fucO"
                     /locus_tag="ROI21_001454"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDX5110394.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(25165..25920)
                     /gene="xni"
                     /locus_tag="ROI21_001455"
     CDS             complement(25165..25920)
                     /gene="xni"
                     /locus_tag="ROI21_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDX5110395.1"
                     /translation="MAVHLLIVDALNLIRRVHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRTAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26031..27398)
                     /gene="sdaB"
                     /locus_tag="ROI21_001456"
     CDS             complement(26031..27398)
                     /gene="sdaB"
                     /locus_tag="ROI21_001456"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDX5110396.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(27456..28745)
                     /gene="sdaC"
                     /locus_tag="ROI21_001457"
     CDS             complement(27456..28745)
                     /gene="sdaC"
                     /locus_tag="ROI21_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDX5110397.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(29301..30665)
                     /gene="ppnN"
                     /locus_tag="ROI21_001458"
                     /gene_synonym="ygdH"
     CDS             complement(29301..30665)
                     /gene="ppnN"
                     /locus_tag="ROI21_001458"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDX5110398.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(30777..31625)
                     /gene="queF"
                     /locus_tag="ROI21_001459"
     CDS             complement(30777..31625)
                     /gene="queF"
                     /locus_tag="ROI21_001459"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDX5110399.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            31693..32238
                     /gene="syd"
                     /locus_tag="ROI21_001460"
     CDS             31693..32238
                     /gene="syd"
                     /locus_tag="ROI21_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDX5110400.1"
                     /translation="MDDLTAQALKDFTACYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            32860..33189
                     /locus_tag="ROI21_001461"
     CDS             32860..33189
                     /locus_tag="ROI21_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDX5110401.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWQNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            33189..33971
                     /gene="truC"
                     /locus_tag="ROI21_001462"
     CDS             33189..33971
                     /gene="truC"
                     /locus_tag="ROI21_001462"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDX5110402.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPIATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            33989..34438
                     /locus_tag="ROI21_001463"
     CDS             33989..34438
                     /locus_tag="ROI21_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDX5110403.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTLLS"
     gene            34873..36225
                     /gene="gudP"
                     /locus_tag="ROI21_001464"
     CDS             34873..36225
                     /gene="gudP"
                     /locus_tag="ROI21_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDX5110404.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            36227..37567
                     /locus_tag="ROI21_001465"
     CDS             36227..37567
                     /locus_tag="ROI21_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDX5110405.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHSGIGEAPGGEVIYQTLVDAIPMVLGQEIARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVSQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            37588..38928
                     /gene="gudD"
                     /locus_tag="ROI21_001466"
     CDS             37588..38928
                     /gene="gudD"
                     /locus_tag="ROI21_001466"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDX5110406.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(39159..41915)
                     /gene="barA"
                     /locus_tag="ROI21_001467"
     CDS             complement(39159..41915)
                     /gene="barA"
                     /locus_tag="ROI21_001467"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDX5110407.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            41972..43273
                     /gene="rlmD"
                     /locus_tag="ROI21_001468"
     CDS             41972..43273
                     /gene="rlmD"
                     /locus_tag="ROI21_001468"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDX5110408.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDNPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLDSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVAGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLH
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            43321..45555
                     /gene="relA"
                     /locus_tag="ROI21_001469"
     CDS             43321..45555
                     /gene="relA"
                     /locus_tag="ROI21_001469"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDX5110409.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRECVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVMANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            45633..45881
                     /gene="mazE"
                     /locus_tag="ROI21_001470"
     CDS             45633..45881
                     /gene="mazE"
                     /locus_tag="ROI21_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDX5110410.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            45881..46216
                     /gene="mazF"
                     /locus_tag="ROI21_001471"
     CDS             45881..46216
                     /gene="mazF"
                     /locus_tag="ROI21_001471"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDX5110411.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            46288..47079
                     /gene="mazG"
                     /locus_tag="ROI21_001472"
     CDS             46288..47079
                     /gene="mazG"
                     /locus_tag="ROI21_001472"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDX5110412.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVIDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            47307..48944
                     /gene="pyrG"
                     /locus_tag="ROI21_001473"
     CDS             47307..48944
                     /gene="pyrG"
                     /locus_tag="ROI21_001473"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDX5110413.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            49031..50329
                     /gene="eno"
                     /locus_tag="ROI21_001474"
     CDS             49031..50329
                     /gene="eno"
                     /locus_tag="ROI21_001474"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDX5110414.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(50389..51261)
                     /locus_tag="ROI21_001475"
     CDS             complement(50389..51261)
                     /locus_tag="ROI21_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDX5110415.1"
                     /translation="MRYFILIFTFVCSFIAAQPTIVPSLQQQVTDLTSSLNSQEKKEL
                     TYKLESIFNNTQVQIAVLIVPTTKDETIEKYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PANPSEIESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSIAVFFFFFTFTTIMVFTCLCVLQKRASGRSYHSDNSGSAGGSDSGGFSD
                     GGGSSGGGGASGRW"
     gene            complement(51275..51416)
                     /locus_tag="ROI21_001476"
                     /pseudo
     CDS             complement(51275..51416)
                     /locus_tag="ROI21_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            51555..52226
                     /gene="queE"
                     /locus_tag="ROI21_001477"
     CDS             51555..52226
                     /gene="queE"
                     /locus_tag="ROI21_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDX5110416.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   52565..52898
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=52565..52593
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
     gene            complement(53536..55014)
                     /locus_tag="ROI21_001478"
     CDS             complement(53536..55014)
                     /locus_tag="ROI21_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDX5110417.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKLLPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     NAMNKGIFKELTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            complement(55041..56318)
                     /locus_tag="ROI21_001479"
     CDS             complement(55041..56318)
                     /locus_tag="ROI21_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5110418.1"
                     /translation="MQHNSYRRWIALAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
BASE COUNT        14380 a        13236 c        14600 g        14375 t
ORIGIN      
        1 ggactaatta tatataacat ttatatgaga taacccacat gaaacaaaag aaagcctgga
       61 gtttttttca gagcctgggg aaggcattta tgtatcccat tgctctgcta agtgtatgtg
      121 gcatgatgct agggctagga agtggtttag ccagtgatga tatggcaaag ttaattccat
      181 ttctggctat tccaataatt aaaaccatac ttgatttcat tgttagcctt ggtttgtttg
      241 cctttgttaa tttacctgta ttgtttgcga tagcaattcc cttaggatta ttaaaagaga
      301 aagaggataa agcctatggc gctttttctg gcttaattgg ttttatggcg atgcatctgg
      361 gaacgaactt ttatcttaaa cagcacgact tattggtcgc tgctgaccaa atgtcgacac
      421 atgggcaaac catcattctg gggatccagt cctacaatac cagcgtgtta gggggaattg
      481 ttgctgggtt attagtcgcc agcatgtata aaaagatcgt taatttacgc attccagaat
      541 cattaggttt ttatagcggc ccacgtctgg tgcctatcat tacactgatt gtgatgagtg
      601 gatttggtct gatcattcct tttatctggc cgccgttttt caatctcttc atgctcattg
      661 gccactggat atcaacttcc ggtcctgttg gttatttctt ctatgcagtt gccgaacgtg
      721 tgacgattcc ttttggctta aaccatctgg tgacgtcagt gttccgcttt acgccaatcg
      781 gcggttcggc tgtgattggc ggcgaagagt attacggcac cctgaacatg tttatggcgt
      841 acgtcaaaga gaatgcggtc attccgctgg atttggcggg gaaaatggag cagggcaaac
      901 tgatgattca gtacggtctg gctggtgctg cactggccat gtatcgcact gctcatgctc
      961 aaaacagaaa ggctatcaaa gcattgctta tttccggtgt gcttacggtt attattggtg
     1021 gcgtcagcga accgattgaa tttctgttct tatttgtcag tccgctgctg tttgtcttcc
     1081 atgcctttat gaatggattc gccaacatgg tcttgccata tatgggcgtg aagatgggat
     1141 ttactggcga tctgattcaa tttattagct ttggcgtatt gcgtggcaca agaacaggtt
     1201 ggccgatcgc ggtgtgtgtc gaagtggcct atttcttcat ttattacttt gtgttccgtt
     1261 ggaccattct caaatttaac ctgatgaccg taggacgtga agagtccagt cctgtcacgc
     1321 tgaacgctca cgaagaaact gcaatagcta atatcccagc ttatgataaa tcagagttgc
     1381 aggcagcgga gcaaatggtt aaggctctgg gtggaaaaga aaatattaaa tcactggata
     1441 attgtgtcac tcgtttgcgt ttaactatcg cagatatggg attgatagat gaggctgcaa
     1501 ttaaaagtgc cggagggatt gcggttgtga aacttgatca aaatacctta caagtcatta
     1561 tcggcactaa agtcatcgcc ctgcgtcggg atatggataa ctacatgggg atacgctgat
     1621 gaatgttttt aataccccta tctcccgtaa aggaacatat tgcactcaat gggacttttg
     1681 tgaggacaga tttggtgtaa aagatgtatt accattttct atttcagata tggatcttcc
     1741 gataccggaa gctatcatca gggcattaaa aaaacgcctg gaacacccga ttttagggta
     1801 cagccgttgg cagcatgatg attatctgaa tgcgatagtc aattggtatc atcaacaata
     1861 taaaactgaa ataaaaccag aatggatcac ctatagccca agtgtgatgt attcaattgc
     1921 taaggcaata gagttattaa cattgaatgg agataacatt ctcattttta cgcctgttta
     1981 taacgcattt tttgatgtaa ttaaacaaag tggtcgaaat atgctaacaa cctctttgaa
     2041 taaagaaaat gagagaggct acgctatcaa ctggcaagat tttgagctaa aaattaaaag
     2101 cgcgaagatg gtgttattgt gtaatcctca taacccgaca ggaaccgtct ggtcggaaga
     2161 agatctcttc aaaatcgctg caagctgcgc aaaacaaaaa atctggctat gttcagatga
     2221 aattcacagt gattttgttt ttaaccatgg tttcacgtcc gcgttaaaaa taaaaaatga
     2281 caaagtagtg gtgttaaatt cgatttcgaa aacatttaat gttcctgcac taacaggatc
     2341 atatatgata tccgctgacc aggattttaa tgataagttt agaacgatat caagatatcg
     2401 cgattttgta aactcaccat ctatattgaa tgtgattgct accattgttg cttataacga
     2461 atgtgaagaa tggttaaagt cgttaaagga tcatattgcc tctaacattc aatttaccca
     2521 gcagtatttg aatgaaaata ttccagaatt gatcgtgagg tccgctgagg gttgctattt
     2581 ttcgtggata gattgctcgg ccattggcta tccctttgat aaattttacc gacgattaat
     2641 tgatgaaggg aaagtgggta ttatggcggg gtatgtttat ggtacggaag gtgagggcta
     2701 tctgcgtctg aatctggcct gcggtcgaga aaaattatat atggggcttg tgcgccttgc
     2761 gagtgtaatt aaaaatatta atcaggaaaa agaaaatgaa taactcggaa cttatacatc
     2821 ttattaaaca ttttatgcgt aacgaacata aggccgttga agaggttatt gattccccac
     2881 tttctgaagt cgctaattta attaaagtat tacaatcttg tcagggtaaa gttgttttta
     2941 ttggtgttgg taagtccggt attattgcca gaaaactcgc agcaacattt gccagtaccg
     3001 gaactccctc gttttttgtt cacggtacgg aagcggtaca cggcgacctt ggaatggtgg
     3061 cgaaagacga tgttgttatt cttatttcaa acagtggtga gacggcggaa atcctggcga
     3121 cactgcctag tctgaaaaga atgggtaatt atttgatctc ttttacccgc agtcatcact
     3181 catcgcttgc gataagttgt gatttatctg ttgaaatccc cgtcaaaagt gaggcggata
     3241 atttaggttt agcaccgtca tgctcgtcta ctgttgttct ggttgttggt gatgcggtgg
     3301 cgcttgcact ctccgaattg aaaaaattta ctcgtgccga tttcggctta tatcatccag
     3361 gaggtgcact cggcattaaa gcaaattcat aatttcaaaa tataaataca gtaaccggtc
     3421 acgccatctt ttggggtggc gttcattgaa gtgagtgtca gctatgaaaa atgttctggt
     3481 caccgtcccc agcttttcag cacgctgtgt ttccgccagc aagttgctaa gagagaataa
     3541 ttttaatctt attattaaga ataatgtgga gcatctgctt aaatcagaat ccactgcatt
     3601 acgagaatct atttgcgcgg tcatcgccgg aaaagattgt tatcaggcgg atacgctatc
     3661 attgctgcct ggtgtgaaaa taatatcgag attcgggacg gggatagaca atattgatct
     3721 tcgtgcggca cagcaatccg gtattgtagt caaaaatgcg gttgggatta attcaaatgc
     3781 ggttgcggaa tttatcattg gacttatctt tgcgagcatg agaaatattc ctggcagcta
     3841 tcatgcgatg caaaatggct actgggttga gtcgcatggc tgtgaattac aagggaaacg
     3901 gattggcctg gttggctacg gtaatatagg taaaaccctg gcgaaaaggt tgtctggttt
     3961 tgatgttgag cttttagctt ttgacaaaca acccgatttt caggccgctg gcaaagatgg
     4021 agttcagttt gtatcaattg aagatatctt tatgcagtcg catgtcatca ttgttctttt
     4081 acctttttct cctgagctgg agaacttcat tagtcataaa tatttgtcaa tgatgcgcaa
     4141 tggtgctctg atcatcaatg ccgcaagggg aaagttgctg gatgagggcg ctttacttca
     4201 ggtgattgaa gaacgaaatg tgtttgcggc gctggatgtg tttagctctg aaccgttggc
     4261 gcaatttagc ccactgctac atgccaaaaa tatcattaca acgccgcata ttgcggccgc
     4321 caccgttgag tcataccagc aaaccgggat acacgttgcc cagtcgataa ttgattactt
     4381 cgcaggaagg gagataaaaa acgtgttgtg agttcagcga acgaagattt taaatacaaa
     4441 aaaaccgcaa tttgtggaac acattgcggt ttatcagcat tctaagtatt ggttgcgggg
     4501 gccggatttg aaccgacgac cttcgggtta tgagcccgac gagctaccag gctgctccac
     4561 cccgcgtcac cgtactgctt ttacttcatc aaattttgat tggttgcggg ggccggattt
     4621 gaaccgacga ccttcgggtt atgagcccga cgagctacca ggctgctcca ccccgcgtca
     4681 ccgtactgct ttacttcatc aaattttaat tggttgcggg ggccggattt gaaccgacga
     4741 ccttcgggtt atgagcccga cgagctacca ggctgctcca ccccgcgtcc gtggatgcgc
     4801 actatactcc gctcgcgttt tcatgcaacc ttttttttaa cccgatcacg gattcggcat
     4861 gattttgaac aaaaacaaat cactaaagcc tattttttgt gcaaaatttg cgtcagggag
     4921 aggctgctca tttcattaaa cgcggtggtt tgttatcttc gttgcgcctt attttttaac
     4981 ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg cacggttgtg
     5041 gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata taaagacggg
     5101 aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc gccgattaac
     5161 gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg tctgtatggc
     5221 aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg tgatacccgc
     5281 aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgccgacaa ctatggtaac
     5341 gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg ccagggcgag
     5401 ttccagtatc ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc tcgtgcggag
     5461 atctacgcgg gggcattgag cgataaatac atcctcgctt acagcaactc cctgatggat
     5521 aacttcatta tggatgtgca gggtagcggg tatattgact ttggtgatgg cagtccgctt
     5581 aactttttca gctatgcagg gaaaaacggt catgcatatc gcagtattgg taaggtgctg
     5641 atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg tcactggggc
     5701 gaaacacaca gtgaagccga ggttcgcgag ctgctggaac agaacccgtc tttcgtcttc
     5761 tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct ggttggccgt
     5821 gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct ggcagaagtg
     5881 ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct gatggtggcg
     5941 ctggatgtcg gtggcgcaat caaaggccaa cacttcgata tctatcaagg gatcgggccg
     6001 gaagccgggc accgcgcagg ttggtacaac cactatggac gtgtctgggt gctgaaaacg
     6061 gctccgggcg caggtaacgt ctttagcggc tgatgtgata ttctgtatgc cgtttttacg
     6121 tatacgacag ggtgagggat aacctcgccc tttttattct gaggtgctat gtctgtggta
     6181 attagtgatg catggcgtca gcgttttggt ggcacagcgc gtctgtatgg tgaaaaagcg
     6241 ttgcaactgt ttgctgacgc gcatatttgt gtggtcggta ttggcggtgt cggttcctgg
     6301 gcggcggaag cgctggcgcg cacggggatt ggcgcaatca cgcttatcga tatggatgat
     6361 gtgtgcgtca ccaataccaa tcggcaaatt catgccctgc gcgataacgt tgggctggca
     6421 aaagcggaag taatggcgga acgtattcgc cagattaacc cggagtgccg tgtaacggtg
     6481 gtggatgatt tcgtgacgcc ggataacgta gcgcagtata tgagcgtggg ttattcgtac
     6541 gtgattgatg ccattgatag cgtacggccc aaagcggcgc tgattgctta ttgtcggcgc
     6601 aataaaatcc cgctggtcac gactggtggc gcgggtgggc agattgaccc gacgcagatt
     6661 caggttaccg atctggcgaa aacgattcag gacccgctgg cggcgaagtt gcgcgagcgc
     6721 ctgaaaagcg attttggcgt agtgaaaaac agcaaaggta agctcggcgt ggattgcgtg
     6781 ttctctaccg aagcgctggt gtacccgcag tcagatggca cggtctgtgc aatgaaagct
     6841 acggcggaag ggccaaagcg gatggattgt gcatctggat ttggtgccgc gacgatggtg
     6901 accgccacct ttggttttgt tgcggtttct catgcgctga agaagatgat ggcgaaagcg
     6961 gcccgtcagg ggtaaacaat caggtcggat gcgacgcgaa cgtcttatcc gacctggttc
     7021 tgtgcttaaa cctgcttcgc cacagcgata atcgcctcgc ttaacgcatt caacccctgg
     7081 ctgcgtgagg cgctaagttg cgcacgcaat cccagctcat caaacaatgc cagcggcgac
     7141 tgtgcctgca attcggcggc ggttttgccc tcaacggcag tcaacaacac cgcaagcagg
     7201 ccgcgcacaa tgcgtccttc gctgtcgcca aagaaatgca tcttgccgtt ttcagccatg
     7261 gtatatccca gccagacgcg gttttcgcat ccggcaatct ctttagcctg cgcttttaac
     7321 tcgtctggca aagccggcag ctgtttcccc agcatgatca actggcgata tttatcttcc
     7381 cattgcgaca acggtgcgaa ggtattgcgt aacgtttctg cggttacggt tgtgccgaac
     7441 ggatgtccgg cgaattgcgg gtttgtcatt aatccaccaa taattccagc gcgcggtcaa
     7501 cggcattcac cagcgcatcc acatcactct ttgtattata tggcgcaaat gaggcacgca
     7561 gtgtgccggt tacgcctaat tctgccagta gcggctgagc gcaatgctgc ccggcgcgca
     7621 gggcaatacc gtactccgcc agcagcgtca ccatatcgct gtgatgaacg ccagcaaaat
     7681 cgaaggccag caggctggaa tcctggcagc ggaatgaacg aaagcctgga cgtttcgcca
     7741 gcgcatcttc cgccagcgtt gctaagctac ggctccagtt ttcagcctgg ttgatatcgt
     7801 aatctgccag ccattccagc gccgcgctta atcctatgac accagcgaca tttggcgttc
     7861 cggcttccag tttccacggc gcagattgag tcgtgaagcc gtcaaaactc acttcgtgaa
     7921 tcattttgcc gccacccagc cagggtgaca tcgcttccag cagttctgat ttgccataca
     7981 gcacgccgat acccgtcggg ccatacagtt tgtgacctga aaaagcatag aaatcaatat
     8041 ccagttgctg aacatccgca gggaaatgca ccgccccctg agcaccatca accatcacca
     8101 ccatcccggc tgaatgagca aaggtaatcg ctcgcgccag atccgggcaa ccgccagtga
     8161 cattcgacat ttgtcccaga gcaagaattc gactacgagg tgtgatcagt tctggcaaca
     8221 aatcgacatc cggcagtcgc tgcgcattaa gcggcaattt caccactttg gatccagttt
     8281 gttgggcgac catcagccag gggacaaggt tggcgtggtg ttctgccacg ctgacaataa
     8341 tctcatcgcc cggttgcaga cgcggacgcg catagcattg tgccaccatg ttgatggatt
     8401 cagtggtgcc gcgcgtccag acgatagttt tatcatccgg tgcattcagt aattgcgcca
     8461 ctttctctcg tgcagcttca taacgcgcgg tcaggcgttg ggcttcggca aactggctgc
     8521 gatggacgtt tccggcgctc aggctgtaaa actgtcgggt ggcttcaacc acggcttcag
     8581 gtttaagcgc ggtcgcggcg ctgtcgagat agacgcccgc atcctgtagt gcgggaaact
     8641 gggcgcgaaa ctgcgcggga ttaaaaacgt tcatggtact cctcggcttg atgcaccgat
     8701 cgtgacgcaa aattcacttt gatacaagga gagtgctaac catcagaatt atctggatat
     8761 cctggcgaag ccgcgtcagt aacatatgct gaatagtgtt aggcgttaat tttagcctgt
     8821 taatcaatgc tattaagatt tattaatcgc tttaaggaga agatgatgaa aaagactgcc
     8881 gcaattattt ctgcctgtat gctgactttt gccctgagcg cctgttccgg ttcgaactat
     8941 gtgatgcaca ccaatgacgg acgtaccatc gtctctgacg gcaaaccaca gactgataac
     9001 gataccggta tgatttcgta taaagacgct aatggcaaca aacagcagat caaccgtact
     9061 gacgtgaaag agatggtcga actggatcag taattcgcga tcgcaaggta aaaaaaagca
     9121 ccgcaattag gcggtgctac attaatcact atggacagac agggtaaatg tacaggaagt
     9181 gaaaaaaggt agctttgcta ccatggtctg aatcgcagac caattgcaaa cacaacaaca
     9241 caacatcaca accgtaagcc aaaagttcac cagaacacgc attccgataa aacttttcgt
     9301 tccggctcag gaagtgccgc cactataggt atttgctggt agaagctcaa cggacaattt
     9361 ataatggctc agattaaaaa aactaatagg ttacacagtg tgatctaatt gttaaattca
     9421 tttaacatca aagtttaaaa gccatgtcta aacgattacc accgctaaat gccttacgag
     9481 tttttgatgc cgcagcacgc catttaagtt tcactcgcgc agcagaagag ctttttgtga
     9541 cccaagccgc agtaagtcat caaatcaagt ctcttgagga ttttttgggg ctaaaactgt
     9601 tccgccgccg taatcgttca ctcctgctga ccgaggaagg gcaaagctat ttcctcgata
     9661 tcaaagagat attttcgcaa ttaaccgaag cgacgcgtaa actccaggcc cgtagcgcca
     9721 agggggcgtt gacggtcagt ttactcccca gtttcgccat tcattggttg gttccgcgac
     9781 tttccagctt taattcagct tatccgggaa ttgacgttcg aatccaggcg gttgatcgtc
     9841 aggaagataa gctggcggat gatgttgatg tggcgatatt ttatggtcgg ggcaactggc
     9901 cggggctacg ggtggaaaaa ctttacgccg aatatttatt gccggtgtgt tcgccgctac
     9961 tgctgactgg cgaaaaaccc ttgaagaccc cggaagatct ggctaaacat acgttattac
    10021 atgatgcgtc acgccgtgac tggcagacat atactcgcca attggggtta aatcatatca
    10081 acgttcagca agggccaatt tttagccata gcgccatggt gctgcaagcg gctattcacg
    10141 ggcagggagt ggcgctggca aataacgtga tggcgcaatc tgaaatcgag gccggacgtc
    10201 ttgtttgccc gtttaatgat gttctggtca gtaaaaatgc tttttatctg gtttgtcatg
    10261 acagtcaggc agaactgggt aaaatagccg cctttcgcca atggatcctg gcgaaagccg
    10321 ctgctgaaca agaaaaattc cgctttcgtt atgaacaata atttacgtag ggtacgacca
    10381 tgaccagccg ttttatgctg attttcgccg ccattagcgg cttcattttt gtggctctgg
    10441 gcgcttttgg cgcgcatgtg ttaagtaaaa ccatgggggc cgttgagatg ggctggatcc
    10501 agaccggtct cgaataccag gcgttccaca cgctggcgat cttaggtctg gcggtggcaa
    10561 tgcagcgtcg catcagtatc tggttttact ggagtagcgt tttcctcgcg ttaggcacgg
    10621 tgctgttcag cggcagcctt tattgcctgg cgctgtccca tctgcgtttg tgggcatttg
    10681 tcacaccggt tggcggcgtg agcttcctcg cgggctgggc gttaatgtta gttggtgcta
    10741 tccgtttaaa gcgcaagggc gtaagtcatg aataaggttg tattgctgtg ccgtccgggc
    10801 tttgaaaaag agtgcgccgc agaaattacc gataaagccg gccagcggga aattttcggt
    10861 tttgcccgcg tgaaagagaa tgcgggttat gtcatttatg aatgttatca acctgatgat
    10921 ggcgataagt taatccgtga gctgccgttc agttcattaa tttttgcccg ccagtggttt
    10981 gtggtggggg aactcctgca gcatttgccg ccagaagatc gtattacccc cattgtcggc
    11041 atgttacagg gtgtagtaga gaagggcggt gaactgcgtg ttgaagttgc cgataccaac
    11101 gaaagcaaag agttgctgaa attctgccgt aaattcaccg tgccgctacg cgctgccttg
    11161 cgcgatgcgg gtgtgctggc gaactatgaa acgccgaagc gtccggttgt gcatgtattc
    11221 tttattgcac caggctgctg ctataccggt tactcataca gcaacaataa ttcgccgttc
    11281 tatatgggca ttccgcgcct gaaatttccg gcagatgcgc cgagtcgttc cacgctcaaa
    11341 ctggaagagg catttcatgt gtttattcct gcggatgagt gggatgaacg cctggcgaac
    11401 gggatgtggg cggtggattt aggcgcttgc ccaggcggct ggacctacca actggtgaag
    11461 cgcaacatgt gggtttattc cgtcgacaac ggcccgatgg cgcaaagtct gatggatacc
    11521 ggacaggtga cgtggctgcg ggaagacggt ttcaaattcc gtccgacacg cagcaatatc
    11581 tcctggatgg tatgcgatat ggttgaaaaa ccggcgaaag ttgcggcatt gatggcgcag
    11641 tggctggtta atggctggtg ccgtgaaacc attttcaacc tcaaactgcc gatgaaaaaa
    11701 cgctacgaag aagtgtcaca caatctggcg tatattcagg cacagcttga tgaacatggc
    11761 ataaatgctc agattcaggc gcggcagttg tatcacgatc gcgaagaagt gacggtgcac
    11821 gtccgccgta tctgggctgc ggtgggtggt cgtcgcgacg agcgataaca ggcaaggccg
    11881 gggcgtcgtc tccggcctgc taccctttcc gtcaggctgt taccaaagaa gttgcaacct
    11941 ggcgctcatc agaaataatg tgcgttacct catccagatg cccgatgcgg gcttcccctg
    12001 aacgattaaa tttactttta tcaatcaata acaacgattg cgcggcgcgt tttaatagca
    12061 ttgatttgta atcagcgttg atggcattgg agtcccacag tgcgccgctg ctatcgatcc
    12121 cttcacaaga aaaaataaac agatcgattt ccagcgattt aagttgggaa atcagcgagg
    12181 gattgacgta acagccatat ttgcgctcaa gtgtgccacc ggaactgatc agttgaatgc
    12241 gttcgcgttt accgagttca tggcaaatcg gatggctatt ggtgaagacc tgaatgttga
    12301 tgtcaggcaa ctggcgtgcc agataccagc aagttgaact ggcgtctaag gctatcacca
    12361 tcccttcttc aatccacgcg agcgcctcgc gcgctatatc tgctttatgc gcataatggc
    12421 ttttcagccg gatgtgaaag ggatcgccac tgtcttgatt ttgacggtgg atatatttag
    12481 cgcgtccatg attgcgcaga attttaccct gcgtctgtaa ttcattgaga tcgcgacgaa
    12541 tggtttcttt actgaccttt agctgttcag agagagcttc cgtggtcagg ctggtatggt
    12601 tcagcagcag gtcgactatc gcttgctggc gtgccgcttt catatttttc accccatcat
    12661 tcgataaccg cccgcgtcat gcgggcgtac cggtatgaga ttacggtgtt accccttttt
    12721 ttaaaagaat attcccgtac ttcgctcgtt cgcctgtgat aacgatcgca aaggcttttt
    12781 gtgcccgttc ataaaacgca aaacgattga tgcggatgat gtcaggacac ggggcttgta
    12841 gtgaaagcgc attacggtaa cgtcgttcta cttcaggatc gagagtgtca ccttctaccg
    12901 ccgccatcat caccagcggc ggtgcataac tgtccagttc aaataatggg ataatcgcct
    12961 ggagcaagtc gctcaccaac aggccatcag cgcggatcac ctgcggtccc atcgaatggg
    13021 cgggaaagtg agcatcggaa aaaataattt catctccatg tcccatctct gccagcactt
    13081 tcaatagttc gggagaaatt aacggcgaaa ttgttttcag catttcacac ttcctctata
    13141 aattcaggtt cagtttgcgg gtagaaataa cggtactgat aatgaatctg cgcgcgagct
    13201 tcctccgggc tgttaaattc ccctacgcca taccaaccga acagcgcagc tcctgcgacg
    13261 gtcgtttcgg cgtcgtcgag aacttttacc ggaatatcaa gcatattggc tttaatctga
    13321 ttccacaatg tgttgcgact tcctccaccg actaacaata attcagaggc tttaaagtgc
    13381 ccgatttttt ccagcatctg tagattgtgc tgtaattgcg cagttaaccc ttccagcgcc
    13441 gcgcgataga aatgcccccg cgtggtatta agcgtcactc cttgccagcc agcgttctga
    13501 cacgacaata aatcacactg cattttcacg ccatccgcgc caggcgcgat cagacgagcc
    13561 tcttcaatca acatttgcca gggagtttca gccgtccaga acagttttct cacccattcc
    13621 agcacgccgg atgccagcca ttgcatacct gggttataca accctgcctg actatccagt
    13681 tcgcaggtcg aaccggtgta ctgacttaac agcgaagtat caacctgggt gctacggacc
    13741 attaaaattt cccatgtacc ggaagagagc acgggttcat tttgttcggc accagcgcca
    13801 aaaagggcga actgggtatc gtgacctgcg gaaatcaccg gtatgccaac gggtaagccg
    13861 agcattgctg cggcgctgtt ctgtaacgta ccaatctgtt cacccgcttc caccagacga
    13921 gggaagagtc ggcgtggaat accggtggct tgtaaaattt gcggactgaa atcgcgttgc
    13981 tggatatcca gcatctggct ggttccggcc atcgtgatat cagtagagaa ttcgccggtt
    14041 aaacggtggt taatcagcga cgaaataaag agccaggcgt gcgcgcgttc cagcagttgt
    14101 ggatgatttt ctttcaacca caccaactta tataacgtat tgaaactaaa ggctccgacg
    14161 ccagaaatag cctgcaaccg ctgtgcggag atcaactgtt caatattgtc cataaccgct
    14221 gctgttcgcg gacatttcca gctaatgatc ggatagagca gattgccttg cttatctacc
    14281 agagcgccat ccacaccaaa ggtggtgacg gcgataccgc ggatgtggca atcagtcagt
    14341 tcactattga tttgccgaca gcaatcagca aagcgttgca aaatggcgtc taaagaccac
    14401 tggtgccagg tgttgttttc catcgcgata tcgctggcat taggcgttga ggcgcgggca
    14461 acaatttttc cctgccgatt aaccgcgatg gccctgacat tggtcgcgcc acagtcgagt
    14521 accaggataa cttcttgttt cataatcgct cctgcaatat agccggataa cattgtttat
    14581 ccggctaacc actcttgctt gttctggccc ggtagccgga gcgaccgggc atcacatcag
    14641 ggagtaatgt attaacgctt gtacaacggg ccgtagttct ggcaagcgcg gtaatcctgg
    14701 ccttcaatat ccatgccgtg cgcggcccag gcagaaggac gatatacttt ggtctcttca
    14761 acgttgtgca tacataccgg gatacgcagc atggaggcga gagtgataaa gtctgcgcca
    14821 acgtggccga tggtcagaac cccgtggtta gcgccccagt tcgccattac cgaatacaca
    14881 tccgtaaacg gccctttacc ggtgaggcgc ggtgcaaacc aggtggttgg ccaggttgag
    14941 ttggtgcgtt tgttgaggat gtcatgcaca tccttcggta actcaacgct ccagccttcc
    15001 gcgatttgca gtaccgggcc caggcctttg atgatgttga cacgggtcat ggtgaacggg
    15061 acgccgcctt cggtaaggaa gcgggaagag taaccgccgc cacggaagta ttcgtggatc
    15121 gccgggcacc attcggtagc ggcgaggcaa gcgtcagcct cttgctgaga gatttcccag
    15181 tgtggcttca tcgtcgggtt accttcgctg tcgcgttgtt tacaggaacc gtccagcgca
    15241 gcagaaccgg agttgatcaa atggatgatg ccgtgttctg ccagtccatc cagtttatgc
    15301 cccgttacac gttcaattgc ttctggtgac cagtaggtac gcacatcggc aaatacctga
    15361 gcggtgccgg tgagctggtg acccattagc attgccacgc cgttaagact gtcgttttcg
    15421 gtcgccacga caaagggttc gcgcacgcca ttccagtcaa atgaactgtt aaggatcgct
    15481 tcggcggtgt cgccattggg atattgatcg gtccagtgac gttgcccctg gaagcccgca
    15541 gcgatggcgt tgtagccaag tgattcttcc acgcgaccaa tatcggccag tttgctgttg
    15601 ccttgcatca tgtcgcggat acacatcgcc atcactaaac tttcgcgcag aactgcgcgg
    15661 ctttgctcgg cattgcgttg atactgtttg ttattttcat cttcgccata gcggaagttt
    15721 ttatcagccc aggccagcgc catttccagt tcggcttcgt cgtaaatctt ctgatcgata
    15781 cggcgacgca gttcggtcat atccaccgcc tggactttca ttcccagcca ggattcaaag
    15841 aagttgtgat caacaatgga accggcgata cccatcgaaa cgccgcccag cgacagatag
    15901 cttttacctt tcatgctggc gacggccaaa ccggcgcggg caaagcgcag cagtttttct
    15961 tcaacatcag caggaatcga tgtgtcatcg gcatcctgaa cgtcatgacc gtaaatggag
    16021 aatgccggga tgcctttctg gctgtgagct gccagagccg cagccaggta aacagcgccg
    16081 gggcgttcag tgccgttaaa gccccaaatg gctttcgggc gggttggatc catgtcgatg
    16141 gtttcactgc catagcacca gcaaggcgtt accgtaatgg tgaggcctac attctggctg
    16201 ctgaattttt cttcgcaagc agcggcttca gccatacccg cgatacaggt atcggaaatg
    16261 acacactcga cggcagctcc gcaggcatgg cgcagtttct cggtcagcag tgcagccgtg
    16321 gctttcgcca tattcattgt ttgttcttca agcgactcac gaacacccat gcgacgaccg
    16381 tcaataaccg ggcggatacc aattttcggt aacgtattaa ttttcatcag gattcctcat
    16441 ctcgctttat tggcgctctg ctaaggcgga gaaaggaaac caccgaagca gtagtattgg
    16501 tcattacttg ctaactcgag ttagcttagg tttgtaggct ttttatgcac aacttttttg
    16561 ttgttgaaat gtgaggggct tcacgagaag tggttaaaat acgatctctg tcaaattatt
    16621 atttagcggg ataaaaataa attttacgta taatcatata gttattatat tttaatggtt
    16681 atgcatcaca gttttaactc aaatgatttg gctaatttgt atacctaaaa tagctttaaa
    16741 aggcgatcgc taactcgagt tagcaaaatg ctaagaatag attgatgcga tgaatattta
    16801 ttcaaaggtt attcgtatca cagtggagat ggctttagta gcgacgttta tgccgtactt
    16861 cacattttca gtttctgatt attgaggaaa gctgcaatgt ccgaaacatt tttgcaaatg
    16921 aaacatatca ccaaacgctt tcctggagtg ttggcgctta atgacgtgca atttacccta
    16981 cgccggggcg aggttcatgc attattaggt gaaaatggtg caggaaaatc taccttaatg
    17041 aaaattttgt cgggggttta tcaacctgat gaaggcgaaa taatttttga agataaacca
    17101 gtctcatttt ctgatccgtt aagtgcgcaa aacgtaggta ttactattat tcaccaggaa
    17161 tttaatttat ttcccgagct tacagtagag gaaaatattt ttatcggcag ggagttttgt
    17221 aaaaaaaatc gctggcgtct ggatgaaaaa cagcaacgcc aggcgacgat tgaaattttg
    17281 caaaaattaa atttggcgat caaaccagat actctggttg ccgaccttac ggtagctcag
    17341 cagcagatgg tagaaattgc caaggccatt tcagttaacg ccaggattct cattatggat
    17401 gaacctactg cggcgttaac agaaactgaa attgaaagct tatttcgcgt cacccggtta
    17461 ttgaaagagc aggggaccgg cattgtgtat atctcccacc gtctggaaga gttggcgcta
    17521 attgccgacc gcgcgactgt aatgcgtgat ggtcagtaca tcagcacggt ggattatgag
    17581 tgcgtaaaaa tcagcgatct gattgccatg atggttggtc gcgatttggg gaatatctat
    17641 ccccgccgcg aggccttgca gcagcggatt ccggttctga aggtaaacgg actgacgcgc
    17701 aaaggcgtcc tgaacgatat taattttacg ctttatcgtg gcgagatcct cggctttgcc
    17761 ggactgatgg gcgcggggcg tactgaactt gcgcgtgcga tttttggtgc ggattccatt
    17821 gacagcggaa cgctaaaatt aaatggtaag gaaaccgtta ttaaagatat ttcggatgct
    17881 attcagcagg gaattagtta cctgacggaa gaccgcaaaa aagaagggct ggcgctgaat
    17941 ttatctgttg aacgaaatat tatgttagga aactaccctg agtattccga tcgttttggg
    18001 aatgtcgatt cgcgtcgttg ccaacaaacc agtgaggaac aggtaaaagc gttaaggatt
    18061 aaaacgccaa atcttgaaca ggcggcatta aatctaagtg gaggaaacca gcagaaaatt
    18121 ataattgcac gttgggtgtg taaagatact gatattttaa tcttcgatga acccactcgc
    18181 ggcattgatg ttggcgccaa attggaaatc tatgagttaa tgaatcgcct ggtggcaaaa
    18241 gggaaatcaa tcatcatgat ctcctctgaa ttgccggagg tgttaggaat gtgcgaccgt
    18301 attttagtga tgcgcagtgg tcgaattacc ggcgaactta gcgcaaaaga agcgacacaa
    18361 gaaaaaatca tgcaatacgc gacgttagag gattaaatta tgactatctc tgtaacctct
    18421 ccagacagta ataataagaa aataaagata aataaagaac ttttgatgcg ccttgcacct
    18481 ctttttagct taattatatt ggtgctattt tttagtttca gctctccctt tttctttaat
    18541 accgaaaata tcatgacgat tgctctacaa acgtcggtaa tcggcattat ggccattggg
    18601 gtaactttcg tcattattac cgctggcatc gatctttcat tgggctccgt ggtggctttt
    18661 tctggtgtcg ccgtaggtat ttgcgccacg ctgggcctgc cgctgcctgt gtgtattatt
    18721 gctggtgttt tggccggtgg attgtgcggc tatgtgaatg gtttgttggt cactaaaatg
    18781 accattccac cgtttatcgc tacgttgggc ctgatgatgt ccgtcagagg catcaatatg
    18841 gtgatgactg acgggcgcgc catctatttt gccgattacc cgatgttcaa aacgctcgct
    18901 cagggacgct tatttgatgt gctgccttat cctgttttct accttgtcat tgtagcgctt
    18961 gtcggcgctt acatcctgaa gaaaaccgtc attggtcgct atgtctatgc cgtaggcagt
    19021 aatgaagttg ctgctcatct ttctggtatt aaagttcagc gcgtgaaaat tttcgtctat
    19081 gccttctgtg gattattaac ggggattgca ggtgtcattc tggcatctcg tctcaactcc
    19141 ggacaaccga cggtaggtgt gggttatgaa ctggaagcta ttgccgcggt agtgattggc
    19201 ggaaccagct taatgggcgg tattggtact attggcggca caatcattgg cgctttcatt
    19261 atgagtgtac tgaaaaatgg cctcaattta atgggcgtct cccagttctg gcaaatggtc
    19321 gcgatgggcg tcgttgtggt cgctgccgtt tacctcgata cattacgcaa aaagattcgt
    19381 tgacttaggc tccctgtttt atcgggaaac aactgccctg acgttaaggg tgattcactc
    19441 agaacggagt acaacaatga aaaccaggca ttttatctat gcgttatctc tgctggcctg
    19501 cgtaacttcc agcgcactgg cgaaggattt aaatcttccg gttgttagta aaggtttcca
    19561 acatgaattc tggcaaaccg taaaaatggg gactgaagct gcggctaaag agttggggga
    19621 taaaaccagc tatgttggcc cggctgatga aacgcaaata gccgagcaaa ttcagttagt
    19681 cgaaaatgcg atggcgcaga aaccgaatgg cttgcttctg gcagccctcg acgcgaatgc
    19741 gctcgcccca ctggtagaaa cggcaaattc acgcggtatc aaagtggtga cctttgattc
    19801 aggcatcaat tcagacattc cggtgagttt tgtcgccacc aataaccgta aagcaggtgc
    19861 tcaggcagct gatgccttag ctgcgcaggt aaataataaa gggaaagttg ggattatcgc
    19921 gcacgttgca ggaacatctt ccgctattga acgctcggaa ggcttcatgg agcgtatgaa
    19981 agaaaaatat cctgacatta aagtcttacc cgttcagtac agcgatggtg acccgcaaaa
    20041 agcgatggat aaaactatcg atatgattca ggccaatccc gatcttgctg ggatctatgg
    20101 caccaatgaa ggttcaacgc tcggggtggc gaatgccatt gatagccaga atctgaaagg
    20161 taaggtgaag gtcattggtt ttgatagtac ggaagcgatt attaacttcc tgaagaacgg
    20221 ggtcattcag ggctttgttg ttcaggatgc ataccagatt ggctatcaag ggatcaaaac
    20281 gttgaacgcg gccctttcgg ggcaagccgt tgaaaaagag atcgatattc ccgtgaaatt
    20341 tgttaatgcc gagaatatta acacgccaga aatcgacaaa ctcctgcacc cattcggtaa
    20401 gaaataaaaa aattggggat gaattctcat ccccgttatc tccagcgtca gggaggcttt
    20461 atgcacaacg gttattttct cggtgtcgat gtcggatcgg ccagtgtcag ggcaggcgtt
    20521 tacagtgcca gtggccacag gctggcattc gctactgcgc ccgtctccca gtttcgccct
    20581 ggcggcgagc gtgtcgaaca gtcttcggca gagatctggc aacaagtttg taaaacggtg
    20641 aaagaggcga cagctttggc aggcatcccc atttccgcaa ttcgctcact gggttttgac
    20701 gctacctgct cgctggttgt tcttgatgaa caaggccagg gtttagcggt ttcgtccggt
    20761 ggatctgcga atcacgacat tattatgtgg atggatcatc gtgcaactgt cgagacggcg
    20821 cagattaacg caacgaagga tcccgcattg cgctacgttg gcggcgaagt gagcgtcgag
    20881 atggagttac caaagttacg ttggttgaag acgcatctac cgcaaacctg gcaggccgct
    20941 catcgctttt tcgatctggc ggattttctg gtctggaaag caacagggcg agacgtcgcg
    21001 ggattgtgca cgctaacgtg taaatggaat tatctggctc atgaacaacg ctttagccat
    21061 agcttactgg aatcggtaga cctgaccgac atgctcgaaa gaattcccgc cgaaattctt
    21121 ccgcctggtg ctgccgtggg aactttaact gccacagctg ctgaagaatt aggattaacg
    21181 accaatgtta tcgtggccag tggtttgatt gatgcccatg ccggtggcat ggcgctggct
    21241 ggcgcgcagc catcaggaac gctggcattg ataagcggaa cgtcgaattg ccatatgttg
    21301 tgcagtgaaa aagaaatatt cacccctggc gtctgggggc catactggtc ggccatgttg
    21361 ccaaattact ggctaactga aggcgggcaa agcgccgcag gggcgctggt ggagtggacg
    21421 cttcaggagt ccggggcaag cgcaaatctt tttaacaagg cgcagcaacg cgggtgtcat
    21481 ccgattcaac tgattaatga atgggtagcc gcactggaag ctaacgagtc agaacccaca
    21541 cgaaatttac atgtgcttgc cgatcatcac ggcaaccgtt cgccacgagc gcggtccgat
    21601 gccagaggca gtgtttatgg cttaacactg gagcgtggtg aaaatcagct cgcgcggctt
    21661 tatcttgcga ctttacaggc aatagccttt ggcaccagac atattattga aactctgcgg
    21721 gaaaatgggc ataccattac ccgattaaca ttatgtggtg gtgcaacgca caacccttta
    21781 tggctacgcg aatatgcaga cgtgacaggt tgcgatatcc acctgatgca agaagaagat
    21841 gccgttacgt tgggcgcagc aattagcggc gctgttgcca gcggagcatg gggggatttt
    21901 acatctgcgt gtaaagcaat ggttgaagcc ggagaggtta ttcaggtaaa tccacaacgc
    21961 cgtgaatttt tagagcgtaa ataccgggtt tacctgacgc tttgggagca acaacaggcg
    22021 gttaaccaac tgatgcaata agacaaaagt tgaaaatact tttatgattg tgatttgacg
    22081 tctggatgtt aactgggaaa tacctatggc taaaacagta gagcagatag ccagcgacct
    22141 gaatttatcg gttacgaccg tgcgactggt gctaaatgga aaagcggaac aatatcgaat
    22201 cagcgtcaaa acccaaacgc gtatcaatga atatgttgaa cgctatggtt atatcattaa
    22261 tcattccgct cgcagcctta agcttaacaa aactgataca ttagggttga ttgttcccaa
    22321 catatccaac gtcttttttg ctacgctggc agaaaaactc gaacaacgct gtcgacgttc
    22381 cggatatcaa cttacaatca gttgcactta tgatgatgtt gactatgaaa ataaattgac
    22441 gaaagcattt atcgcacgaa atgtagatgg tctttttatt gttccttcga cgctggaaaa
    22501 tcagcagcat catttacgca aaattcgtaa gccaatggtt ttactcgatc gtgattttaa
    22561 atataccgat aacgcattgg tagagagtaa taatatttct ggcggagaaa agctgacgca
    22621 aaatattctt gaggccgggc aggctccagt atggtttttg ctgggagatg ccggtttgcc
    22681 gagtatcagc gatcgtctga caggctattt gaatgcgttg gcaaaaaaag ggattaccca
    22741 tcgcgattgg gtgcgggaag gtccggtaaa tactccggaa ggcggctatg tcattatgga
    22801 aaaactcatt gaagagcagg gatgtccgca ggcatttatc gcctcgtctt tacctgtgct
    22861 tgaaggcgct gtccgcgcga ttcgcgaccg tcttggcgct gtaccgccag aaatcaatat
    22921 tggcactttc gatgaacatc ctatgctggg atttctcgcc aacaatgtct ggtcaatgca
    22981 gcaggatgaa aatgcctggg cagaaaaagc gttcgaaatg atgttaagcg cgatcgaaga
    23041 aagaccagtc aaaaaaacag ttaaagtaga aatgaaatta attaagcgca taagaaaaaa
    23101 ataataatcg ccaaaagata tcaggctact gaacaatgat attcagtggc ctccccttac
    23161 taaaaataac cccgtcttca tttgcggtgc tgttttacgc aacaaggcga gctgaaactg
    23221 tatctggaga atagaaatgg aaagaacacg tttatcccgt gaaataattg aaacctgtct
    23281 ggaaatgacg cgccttgggt taaatcaggg gacagcgggg aatgtcagca cacgttttga
    23341 aaacggcatg ttgattactc ccagcggtat tccttatgaa agaatgacgg agaatatgat
    23401 tgtctatgtt gatggcaacg gcaaatacga agaaggtaag attccttcca gcgaatggcg
    23461 tttccatatg gcggcctatc aaagcagacc ggatgccaac gcggttgttc acaatcatgc
    23521 cgttcattgc acggcagttt ccattcttaa ccgaccaatc cccgctattc actacatgat
    23581 tgcggcggct ggcggtaatt ctattccttg cgcgccttat gcgacctttg gaacacgtga
    23641 actttctgaa catgttgcgc tggctctcaa aaatcgtaag gcaactttgt tacaacatca
    23701 tgggcttatc gcttgtgagg tgaatctgga aaaagcgtta tggctggcgc atgaagttga
    23761 agtgctggcg caactttacc tgacgaccct ggcgattacg gacccggtgc cagtgctgag
    23821 cgatgaagag attgccgtag tgctggagaa attcaaaacc tacgggttac ggattgaaga
    23881 gtaatttcgt aaagcaacaa ggagaaggat gatggctaac agaatgattc tgaacgaaac
    23941 ggcatggttt ggtcggggtg ctgttggggc tttaaccgat gaggtgaaac gccgtggtta
    24001 tcagaaggcg ctgatcgtca ccgataaaac gctggtgcaa tgcggcgtgg tggcgaaagt
    24061 gaccgataag atggatgctg cagggctggc atgggcgatt tacgacggcg tagtgcccaa
    24121 cccaacaatt actgtcgtca aagaagggct cggtgtattc cagaatagcg gcgcggatta
    24181 cctgatcgct attggtggtg gttctccaca ggatacttgt aaagcgattg gcattatcag
    24241 caacaacccg gagtttgccg atgtgcgtag cctggaagga ctttccccga ccaataaacc
    24301 cagtgtaccg attctggcaa tccccaccac agcaggcact gcggcagaag tgaccattaa
    24361 ctacgtgatc actgacgaag aaaaacggcg caagtttgtt tgcgttgatc cgcatgatat
    24421 cccgcaggtg gcgtttattg acgctgacat gatggatggt atgcctccag cgctgaaagc
    24481 tgcgacgggt gtcgatgcgc tcactcatgc tattgagggg tatattaccc gtggcgcgtg
    24541 ggcgctaacc gatgcactgc acattaaagc gattgaaatc attgctgggg cgctgcgagg
    24601 atcggttgct ggtgataagg atgccggaga agaaatggcg ctcgggcagt atgttgcggg
    24661 tatgggcttc tcgaatgttg ggttagggtt ggtgcatggt atggcgcatc cactgggcgc
    24721 gttttataac actccacacg gtgttgcgaa cgccatcctg ttaccgcatg tcatgcgcta
    24781 taacgctgac tttaccggtg agaagtaccg cgatatcgcg cgcgttatgg gcgtgaaagt
    24841 ggaaggtatg agcctggaag aggcgcgtaa tgccgctgtt gaagcggtgt ttgctctcaa
    24901 ccgtgatgtc ggtattccgc cacatttgcg tgacgttggt gtacgcaagg aagacattcc
    24961 ggcactggcg caggcggcgc ttgatgacgt ttgtaccggt ggcaacccgc gcgaagcaac
    25021 gcttgaggat attgtagagc tttaccatac cgcctggtaa atgcgctgat gtgataatgc
    25081 cggatgcgac gctagctgcg tcttatccgg cgtataagtt cattaaattc aatgtgttga
    25141 taatcgtttg catccggcac gccgttaccg taccaaccgc aattgctgta aattgccgtc
    25201 gatatgcaaa tcggtttgta agcgggcaat atcgcggcac agaaacgcca tctctttatg
    25261 ggtttctaat tttttgcgcc acttttcggc aaccgcatcc agattctcat atatcccttc
    25321 cagactctga aactcgacca gcagctgcgt ggcgcttttg ggtccgatcc ccgcaacacc
    25381 cggtaccttt gaactgctga tccccgcaag cccccagtaa tcgggcaact gctgcggttg
    25441 aacgccaaat tctttatcga taaatggcgc atccagccaa cgtttctgga agtaatcacg
    25501 aatacgtaat gtcggtgaaa gtaactgaca gtagccttta tctgtcgaaa caatcgttgc
    25561 ctgatgcccg gcctgtgtca ctttgaccgc cagcgtggcg gctaagtcat cggcttcgtt
    25621 accgctggct gaccagcacg ggacgccgcg ttgctcaaag gcggtgcgta gtgcaggcat
    25681 ctcgtcgtgc aactcttccg gcattggcgg tcgacccgct ttgtaatctg gtaaacgctg
    25741 atgacgccag ccgctactgc ggttttcatc atcaaaaacg gcgaccgcgt gggttggctg
    25801 gctgtgcata atgagctgat cgagcgcgtg ctggcaggtt tcgacacagg gcgaaccctg
    25861 aacggcatga acacgacgaa taagattcag tgcatcgaca ataagcaaat gaacagccac
    25921 gataaccccc ttaaagatca ggcgataagg gtaacattcg atgccggaac aggctatggc
    25981 tggttgagaa aacgggaaga ggcctcgcaa aacgaggcct ccgggagaga ttaatcgcag
    26041 gcaacgatct tcattgccag gccaccgcga gaggtttcgc ggtacttggc gttcatatct
    26101 ttacctgttt cgtacatggt ctcgataact ttatcgaggc agacgcgcgg ctcgctggta
    26161 cggcgcagcg ccatacgcgc ggcgttcacc gcttttactg ccgcaatggc gttacgctcg
    26221 atgcacggta cctgcacctg tccagcaacc gggtcacacg tcagaccgag gttatgctcc
    26281 atggcgattt ccgccgcgat gcacacctgc gccgggcttg cgcctaacag ttctgccaga
    26341 ccagctgccg ccatagagca cgccacgcca acttcaccct ggcaacccac ttcggcacca
    26401 gaaatcgacg cgttcatctt ataaagagaa ccaatggcgc tggctaccag caggtaacga
    26461 gccagtgagt tagcgttcac ttcgcggata aacttatcgt agtacgccag cacagccggg
    26521 ataatcccgc acgcaccgtt agtcggcgca gtcaccacgc gaccgccagc agcgttctct
    26581 tcgttcactg ccagtgcaaa catgttgatc cagtcaacaa ccgccatcgg gtcagtggtg
    26641 gttttatcct ggctgaccag catccggcgt agtgccgcag cacggcgtgg tacgcgcagt
    26701 ttgccaggca acacgccttc ggtggaaata ccgcgctcaa taccgccgcg catgacttcc
    26761 cagacgttcg ccaggtgctg ttccagctct tctttgctgt gcagcgccag ctcgtttttc
    26821 atcatcaggc cagagagtga cagcccggtt tcctgacaat gtttttgcag atcggctgct
    26881 gaactgtacg gataaggaac ttcaaccggt gcgctatcct gctggccaaa atgttcttca
    26941 tcaacgataa agccaccgcc gatagagtag taagtctggc tgtaaacgac tttatcgccc
    27001 gccagcgcgg taatgcgcat accgttttca tgcagagaaa ggttgtcggc atggaagttc
    27061 atgcactgat caaccgggaa ctccacttca tgctgaccat ttgccagcat caggcgacca
    27121 tgagtattca catcctgaat aaaaccaggg atggaatcga tatccacggt atccggcagg
    27181 ttacccgcca ggcccataat aatggcgata tcagtgtggt ggcctttacc agtcagagag
    27241 agcgagccgt acacgtcaac caccacgcgg gtcacgtctt taagcaggtt acgggcaatc
    27301 agatcgtcgg taaattgttt acccgctttc attggtccaa cggtatgaga actggaaggg
    27361 ccaatgccga ttttgaaaat atcgaatacg ctaatcatag gaaatacatc gcgttaaaac
    27421 ggaggaagcg ccgcccgaaa gcggcgcgaa aggacttagc tgaacagaga gtagaagatt
    27481 gcggagattg caatcagacc catcacgaca acgaatacgt tgctgatgtg accgctgtac
    27541 ttacgcattg ccggtacttt ctgaattgcg tacatcggca tcaggaacag gatcatcgcg
    27601 atgattgggc cgcccagggt ttcaatcata cccaggatgc tcgggttcag ggtggcaaca
    27661 atccaggtcg ttaccagcat gaacagcgca gtgatacggt tcagcttgtt gatttcgata
    27721 gacttacctt taccacgcag agatttaatc accataccgt tgaagccttc acgtgcgccc
    27781 aggtagtggc cgaggaagga tttggtgata gcgataatcg cgataatcgg tgccatccac
    27841 gcgataaccg gtgcgttaaa gtggttagcc aggtaagaca gaatcgagat gttctgctct
    27901 ttagccgcag ccaggtctgc cggagtcagg ctcagtacgc agctgaagac gaagaacatt
    27961 acggtcagca ccatcatgat gtgtgcgaat gcaaggatct tcgagcattt ctgttctgcc
    28021 atatcgccgt actcttcacg cttcgcaacg gcgaaagaag agatgatcgg agagtggttg
    28081 aacgagaaca ccattaccgg aattgccagc cacagggtca tccacagacc gtttccggtt
    28141 gccgatgcag tgtccagaga cagcgtttcc agtgctgcgc cgttccactg cgggatcagg
    28201 tacagagcca gcagcatcag tacgccaaca aacgggaata ccagaatact catcgcttta
    28261 acgatcatct gctcaccgaa gcgaacgatg gtcatcatac ccacgatcag gatcagcgac
    28321 agaatcgcac gcggcggtgg tgtcataccc agctggtgag acatgaagct ttcaactgta
    28381 ttggtgattg ccacgctata aaccagcagg atcgggtaga tagcgaagaa gtagagcagg
    28441 gtaatcagtt tacctgcgcc aataccaaag tgttcttcta caacctcggt gatgtcttcg
    28501 cctgggtttt taccagacag tacgaagcga gtcaggccgc ggtgagcaaa aaatgtcatc
    28561 gggaacgcaa ggatagccat gatgatcagc gggatcatac cgccaacacc ggcattgatt
    28621 ggcaggaaca gcacgcccgc gccgattgcc gtgccgtaaa ggcccagcat ccacatggtg
    28681 tctgtcttgc gccaggcact acgagagtct ttcgacgcaa tcgtgctggt ttgagtcgtt
    28741 tccatctatt tctcctggag gaatgcaaat aattcagctt ttagccagtt cagcccggga
    28801 aggtgcctga ccgtaaaaat gaaatctgtc tatcggcgat ggcttaatca tttaccgccg
    28861 taataatttg agggcggaaa gatacacaca agttatgtat ctatcagtga tcttgatccc
    28921 aaatacaata atctacttgg aagatagatt atcccaacgc tttactctgt aaatcaaaag
    28981 taaaagagaa tttaagggca atatttgccc attaaacgac atctgacttc ttccggggcg
    29041 cgcaatatat gtaggttgaa ctgaaaaaat caatactttt agttaatcat ggcgataatt
    29101 tctttttcat agcgataaaa tgtggtttat gaaaataggt aaacgtttgc gttacaacat
    29161 aaattgtatg acatattaac tgtaatgata tttatcgcgt gagggtggta agaaacattc
    29221 atataacaaa tgaaataaat tgatgggtat gtgaccaaaa acgagaaaac ccggcaacaa
    29281 acgtcaccgg gggagggaga ttacgtgcag atttcgtagc aagggatgta ggctgagcct
    29341 ggcaacttca tacgatgctg ggcaacaaaa ccctgtagca ggtcatccat acgacgcata
    29401 atctctttat cgccgttgat tttataagga ccaaactctt caatggcgcg aataccgact
    29461 tcttttacgt tacccgccac aataccggag aacgcacggc gcaggtcggc agccagcact
    29521 tcaacgggtt gatccgggta aagcttcaga ttagccatat tctcgtgaga cggctcaaac
    29581 ggcatttgca aatctggcgc aatgcgcatt gaccagttaa agctgtaggc atcgcctgtg
    29641 tcgcggcgat tttctttcac cagcggcatc gattttttca tctgacgagc gacttcagcg
    29701 gcgtcatcaa tgatgatgcg gtaatggcgg cgcgcgtttt cacccaacgt atgcacgaca
    29761 aactcgtcca gtacgcggaa gtagtcggcg ctctctttcg ggccggtgag gatcaatggt
    29821 aaaacctgat ctttgttggc cgggttcatt aaaattccca gcaaatacag cagctcttct
    29881 gccgtaccca caccgccagg gaagataatg ataccgtgag cgatacggac aaacgcttcc
    29941 agacgttttt cgatatctgg catgatgatc aattcgttga ccagcgggtt aggcggttca
    30001 gcggcgataa tcgacggctc tgtcatacca ataaaacgac tgtctttgta acgctgctgt
    30061 gcgtgtccga ccgcagcgcc tttcatcggc gcttccatcg ctcccggacc acagccggtg
    30121 cagatattca gctcacgcag gcccagctga ttcccgacgc gacgggcata caaatactcg
    30181 ttttcgttaa ttgagtgccc gccccagcag accaccatat ttggcgcttc accgacatgc
    30241 agtgcgcgag cgttacgcaa gatggaaaag accaggttag tgatgtggac ggagttgtcg
    30301 agattcaggt tgggaaaacg aacggtgtta tggatttgcc cgtaaacgaa gagaatgtca
    30361 cgcagaaccg cgaacaagtt ggcctgcaaa gcgcgaataa ttcgcccatc gacaaaagcc
    30421 tcttccgggg gattaatcag ttccagcttt acgccgcgtt cacggcgcag gacgttaata
    30481 tcgaaatttt caaaacgaga cagcaattct ttgctgttat cggtcaaact accggagttc
    30541 agtacggcaa gtgaacagtt gcgaaacagt tgatagaggt cgctgctggc ggtgcgttta
    30601 agcatatcca cttccagctg cgacaacata tccatggagc caagcgggct aatatgtgta
    30661 atcaagaaaa actccttatg ggacgaatat cgtcattccc tttgattaca atagccctgc
    30721 ctgatgacct ttcacaacct tctgcgtgaa tccagcacgc aaaattgaga aaaaatttat
    30781 tgccgaacca gtcttgtggt cgatgggaca aaatcgctat tactgcgcca cgggttaatg
    30841 tccagaccgc cacgacgggt ataacgtgcg taaacgctca atttctctgg ctggcagaag
    30901 cgtaacaggt cattaaagat gcgttccacg cactgttcgt ggaactcgtt gtgatgacgg
    30961 aaagaaacca ggtaacgcag cagtttttct ctgtcaattt ggcgtccacg atactgaatt
    31021 tggatcgatc cccaatctgg ttgatgggtg atcaggcaat ttgatttcag caggtggctg
    31081 acgagcgtct cttctactac tttttcgcca ctggtggcat tctccagata gtcagtagtg
    31141 aattcatagt tatcgatagt gatatcctgg tcatcaatgc aagtgccatt gaaatgacct
    31201 atcggttggc cttccagttc atcaagacga tataacgcca cgctaacctt accctgagcg
    31261 caagtgctta agtcgcgctc cagcgtctgg cgcacctcat cccagttatt aaaacgcgtc
    31321 tggttaaaac tgttgagata gagcttaaaa ctcttcgact caatcagatt tgcgctggtg
    31381 taatcaagtt caacatgacc gacggcgacc tgcggcaaac ctttcgcatt cagccaggaa
    31441 agttcatata gcgtccagat atccgtaccg tgaaaaggca ggttatccgc tttcagaccc
    31501 agcggatcgc gattcagact acgcggaacg ccttgcagta ggctggcgtc ataggtatcc
    31561 cggtaatcgg ttgattttcc aagagtcagg cccgcaagtg cctgatggtt tgcataagaa
    31621 gacatgtttc accgtcataa accagataaa ctaaacgaca agtgtatctg gtttaagtga
    31681 agagagaaat tagtggacga tttgaccgca caagccctga aagattttac tgcatgttac
    31741 tgcgatgcgt ggcatgaaga gcataaaagc tggccgttga gtgaggaact gtacggcgtt
    31801 ccttcaccgt gcattatttc aactaccgaa gatgccgtat actggcaacc ccagccgttt
    31861 accggggaac aaaatgtaaa cgcggttgaa cgcgcttttg atattgtgat acaacccacg
    31921 attcatacct tttatacgac tcagtttgcc ggggatatgc acgcgcagtt tggcgatatc
    31981 aaactgacat tgctgcaaac ctggagtgaa gacgatttcc gtcgggtgca ggaaaatctg
    32041 attggtcatc tggtgactca gaaaaggcta aaactgccgc ccacgctatt tatcgcgacg
    32101 ctggaagaag agttagaggt catctcggtg tgtaatctct ccggtgaagt gtgcaaagag
    32161 acgctgggaa cacgtaaacg gacgcattta gcctcaaatc ttgcggaatt ccttaatcaa
    32221 cttaagcctc ttctgtaatc caaatacccc atctggttgt gagagatctc ttacagactc
    32281 tgtaggagat cgccaggaaa taagcgaata cttaaaaaga taagaatcgt tattttcatt
    32341 taaaatcaat atattgcttg tcaattctta actttcatat gaaattttcc ttaaggcatt
    32401 gtctgtaagc gccttgtaag tcttcgcgaa aaagacgatt ctatcttcgt cgacagggag
    32461 tcagacaacg aagtgaacat caggatgatg acacttctga aggacacacc aggatggtgt
    32521 ttcagggaaa ggcttctgga tgaagcgaag aggatgacgc aggacgcgtt aaaggacacc
    32581 tccaggatgg agaatgagaa ccggtcagga tgattcggtg ggtcaggaag gccagggaca
    32641 cttcaggatg aagtatcaca tcggggtggt gtgagcagga agcaatagtt caggatgaac
    32701 gattggccgc aaggccagag gaaaagttgt caaggatgag cagggagcaa caaaagtagc
    32761 tggaatgctg cgaaacgaac cgggagcact gtgaatacag tgctcccttt ttttattcct
    32821 gctatccttc gcggcagttt ttcttattga ggttgcttta tgaccactca tgaccgcgtt
    32881 cgtctccagc tgcaggcgct tgaagcatta ctgcgtgaac atcagcactg gcaaaacgat
    32941 gaaccccagc cgcatcaatt taacagtacc caaccgttct ttatggacac catggaacca
    33001 ctggagtggt tgcagtgggt gctgatcccg cgaatgcacg atttgttaga taacaaccag
    33061 ccgttaccgg gcgcttttgc cgttgcaccc tattatgaaa tggcgctggc gacggaccac
    33121 ccgcaacgcg cgcttattct ggctgagctg gaaaaactgg atgctctttt tgcggatgat
    33181 gcgagctaat gctggaaata ctctatcagg atgaatggct ggttgcggta aataaaccct
    33241 ccggctggct ggttcaccgc agctggctgg atcgcgacga gaaagtagtg gtcatgcaaa
    33301 ccgtgcgtga ccagataggc cagcatgtgt ttactgctca tcgtctggac cgacccactt
    33361 ctggtgtgtt gttgatggga ttatccagcg aggccggacg gctgctggca caacagtttg
    33421 aacagcacca aatccagaaa cgttaccatg cgattgtgcg cggctggttg atggaagaag
    33481 cagttctgga ttatccactg gtggaagaac tggacaaaat cgctgataaa tttgcccgcg
    33541 aagataaagg cccacagcca gcagtgacgc attatcgcgg tctggcgacc gtagaaatgc
    33601 cgatagcgac cggacgttac ccgaccacgc gctacggcct ggtggaactg gagccgaaaa
    33661 ctggacgtaa gcaccagctc cgtcgccatc ttgcccattt gcgccacccg attattggcg
    33721 acagcaaaca tggtgattta cgccagaatc gcagcggtgc tgaacatttt ggccttcagc
    33781 ggttaatgct gcatgccagt cagttgtcat tgacgcatcc ttttactggc gagccgctga
    33841 ctattcacgc gggcctggac gacacctgga tgcaggcgtt atcacaattt ggctggcggg
    33901 gtctgcttcc tgaaaatgaa agggttgagt tcagtgcgcc atcgggtcag gatggagaga
    33961 taagttccta atccagggag aaaacggtat ggcggaaatt ggtatttttg tcggcactat
    34021 gtacggaaat tcgctgttag tggcggaaga agcggaagca attctgaccg cgcagggcca
    34081 caaagcaacg gtatttgaag atcctgaatt aagcgactgg ctgccctatc aggataagta
    34141 tgttctggtg gttacgtcca cgaccgggca gggcgacctt cctgatagca ttgtgccgct
    34201 ctttcaggga atcaaagata gtctgggctt ccagccgaat ctgcgttatg gcgtgattgc
    34261 gctcggcgac agtagttatg tgaatttctg caatggcggc aaacagttcg atgccttgtt
    34321 gcaggaacag agcgctcagc gggttggcga aatgctgttg attgacgcca gtgagaaccc
    34381 ggaaccggaa acggaatcaa atccgtgggt cgaacattgg ggcacgctgt tgtcctgata
    34441 cgttcagcga gcggtaaatg ccgttttagc ggtgctgaat cgaatctttt caggcaaatg
    34501 ccagtaaaaa ctgcttcata gcgcggattt ttactggcgt ttgcctggag tcaagcgatc
    34561 catttcatac tcttctttat ttcttcgttt taacccttcc tttcttgttc ttgttttcat
    34621 ttccgtgaag tggattccac cgtccagggc taatgccaaa atcgggcctc attgaacgca
    34681 ttaatgttgt gttgttgcac ggtgagccgc tatggcgcgc tttttatact gctattgcca
    34741 gatataaaca cgcgccgtat tcggcgaacg acctataaaa acggcaaaaa acaccctacg
    34801 tcacctctga tttcctggcg atgtcgcagt ccagagtgag cgtggctaac gcgaattttc
    34861 aggagtgcaa caatgagttc tttaagtcag gctgcgagca gtgtggaaaa acgcacaaat
    34921 gctcgttact ggatagtggt gatgttgttt atcgtcacat ccttcaacta cggcgaccgc
    34981 gctacgcttt ctattgccgg ttcggaaatg gccaaagata tcggccttga tcccgtggga
    35041 atgggctatg tgttctctgc tttctcatgg gcttatgtta tcggacagat ccctggtggt
    35101 tggttgctgg atcgttttgg ttcaaaacgc gtctacttct ggtcgatctt tatctggtcg
    35161 atgtttacct tgctgcaagg cttcgtcgat atttttagtg gattcggcat tatcgttgcc
    35221 ctgtttacgc tgcgcttcct ggtcgggctt gctgaagcgc catctttccc cggcaacagt
    35281 cgcattgttg cggcctggtt tccggcgcag gaaaggggaa cggcggtgtc gatttttaac
    35341 tccgctcaat acttcgcaac ggtgatcttc gcgccgatta tgggctggct gacgcatgaa
    35401 gtgggctggt cacacgtctt cttctttatg ggcggtctgg gcattgtcat cagctttatc
    35461 tggttgaaag tcatccacga gccaaatcaa catccgggtg taaatcagaa agagctggag
    35521 tacatcgccg cgggtggcgc gctgatcaat atggatcagc aaaataccaa agttaaagtg
    35581 ccgttcagcg tgaagtgggg gcagatcaaa cagctgctcg ggtcacggat gatgatcggc
    35641 gtttatatcg gtcagtactg tatcaacgcc ctgacttact ttttcattac ctggtttccg
    35701 gtttatctgg tgcaggcgcg tgggatgtcg attctgaaag cgggctttgt ggcttccgtt
    35761 ccggcggttt gcggttttat cggcggtgtg ctgggtggga ttatttccga ctggctgatg
    35821 cgccgcacgg gatcgctgaa cattgcgcgt aaaacaccga tcgtaatggg catgttgctg
    35881 tcgatggtga tggtgttctg caactacgtc aacgttgagt ggatgatcat cggttttatg
    35941 gcgctggcct tcttcggtaa gggcatcggg gcgctgggtt gggcagtaat ggcagatacc
    36001 gcgccaaaag agatcagcgg tctttccggt ggcctgttca acatgttcgg taacatttct
    36061 ggcatcgtca cgccaatcgc aattggttat atcgttggca cgactggctc gtttaatggg
    36121 gcgctgattt atgttggtgt tcatgcctta atcgcggtgc tgagctacct ggtgctggtg
    36181 ggcgatatca agcgtatcga gctgaaacct gtcgcggggc aataagatga cgacacaatc
    36241 cagtcctgtt attactgata tgaaagtcat tccggtggcc gggcatgaca gcatgttgct
    36301 taatattggt ggtgcacata acgcatattt cacccgcaat atcgtggtac tcaccgataa
    36361 tgccggacat tccggcattg gtgaagcgcc aggcggagag gtgatttatc aaacactggt
    36421 cgatgctatt ccgatggtgt tgggccagga gatcgcacga ctgaataaag tggttcagca
    36481 ggtgcataaa ggtaatcagg cagccgattt tgataccttc ggcaaaggtg cctggacttt
    36541 tgaattgcgc gttaatgccg tggcggcgct ggaagccgcc ttgcttgacc tgctaggtaa
    36601 ggcgctgaat gttccggtct gcgaactgtt agggccaggc aagcaacgcg atgctattac
    36661 cgtcctcggt tatctgtttt atatcggtga tcggaccaaa accgatcttc cttatctgga
    36721 aaatacgccg ggcaaccatg agtggtatca attgcggcat cagaaagcga tgaacagcga
    36781 agccgttgtg cgtctggcgg aagcttcaca ggatcgctat ggctttaaag atttcaaact
    36841 taagggcggc gtgttacctg gcgagcaaga aatcgacact gttcgtgcat tgaagaaacg
    36901 cttcccggat gcgcggatta ccgttgatcc caacggagca tggctgcttg atgaagctat
    36961 ttctctgtgc aaagggctga atgatgttct cacctatgcc gaagatccat gtggcgcaga
    37021 acagggtttc tccggacgtg aagtgatggc ggagtttcga cgggcgaccg gcttgcccgt
    37081 cgcgactaac atgatcgcca ccaactggcg cgaaatgggc catgcggtga tgctcaatgc
    37141 ggtagatatt ccacttgccg atccgcactt ctggacgctt tccggtgcag tccgcgtgtc
    37201 gcagctttgc gacgactggg ggctgacctg gggctgccat tccaataacc actttgatat
    37261 ctctctggcg atgtttaccc atgtgggtgc ggcggcaccg ggtaatccta ccgctatcga
    37321 tacccactgg atttggcagg agggcgattg tcgcctgacc aaaaatccgc tggagattaa
    37381 aaacggaaaa attgccgttc ctgatgcgcc cggtctgggc gtggaactgg actgggagca
    37441 ggtacaaaag gcgcatgagg cctataaacg tctgcctggc ggtgcgcgta acgacgcggg
    37501 cccgatgcag tacctgatcc ccggctggac ctttgaccgt aaacgtcccg ttttcggccg
    37561 tcattgattt tgaaaaagga cataaatatg agttctcaat ttacgacgcc tgttgttact
    37621 gaaatgcagg tcatcccggt ggcgggccat gacagtatgc tgatgaatct gagtggtgca
    37681 cacgcaccgt tcttcacgcg taatattgtg attatcaaag ataattctgg tcacactggc
    37741 gtaggggaaa ttcccggcgg cgagaaaatc cgtaaaacgc tggaagatgc gattccgctg
    37801 gtggtgggta aaacgctggg tgaatacaaa aacgttctga cgctggtgcg taatacattt
    37861 gccgatcgtg atgccggtgg gcgcggtttg cagacatttg acctgcggac cactattcat
    37921 gtagttaccg ggatagaagc ggcaatgctg gatctgctgg ggcagcatct gggggttaac
    37981 gtggcatcgc tgctgggcga tggtcaacag cgtagcgaag tcgaaatgct cggttatctg
    38041 ttcttcgtcg gtaatcgcaa agccacaccg ctgccgtatc aaagccagcc ggatgataaa
    38101 tgtgactggt atcgcctgcg tcacgaagaa gcgatgacgc cggatgcggt ggtgcgcctg
    38161 gcggaagcgg catatgaaaa atatggcttc aacgatttca aactgaaggg cggtgtactg
    38221 gccggggaag aagaagcaga atctattgtg gcactggcgc aacgcttccc acaggcgcgt
    38281 attaccctcg atcctaacgg tgcctggtcg ctgaacgaag cgattaaaat cggtaaatac
    38341 ctgaaaggtt cgctggctta tgcagaagat ccgtgtggtg cggagcaagg tttctccggg
    38401 cgtgaagtga tggcggagtt ccgtcgcgcg actggcctgc cgaccgcaac caatatgatc
    38461 gccaccgact ggcggcagat gggccatacg ctctcgttac aatccgttga tatcccgctg
    38521 gcggatccgc atttctggac gatgcaaggt tcggtacgtg tggcgcaaat gtgccatgaa
    38581 tttggcctga cctggggttc acactctaac aaccacttcg atatttccct ggcgatgttt
    38641 acccatgtag ccgctgctgc accgggtaaa attactgcta ttgatacgca ctggatctgg
    38701 caggaaggca atcagcgtct gaccaaagaa ccgtttgaga tcaaaggcgg gctggtacag
    38761 gtgccgcaaa aaccaggatt gggcgtagaa atcgatatgg atcaggtgat gaaagcccac
    38821 gagctgtatc agaaacacgg gcttggtgcg cgagacgatg cgatgggaat gcagtatctg
    38881 attcctggct ggacgtttga taacaagcgc ccgtgcatgg tgcgttaaga cagaaaacca
    38941 aaaccggctc tttcaggagc cggtttgaga ctgataccaa acgcaaaatt gcctgttgcg
    39001 ctacgtatca ggccaacata ctttttaatt tattgaattt aattggtttt gtaggccgga
    39061 taaggcgtta acgccgcatc cggcataaac acaggcacct tgtcaccaat ctgaaaccag
    39121 cgtcataaaa agccgattgc tactcgacaa gacgtccgtt acccgagaat tttgctggct
    39181 tcgcgcgcta cattatccat ctcgtctaac agttccagca gctccggctc caaatcttct
    39241 tctttagtac cgctacgtag ctgttgttcg atcagttggc agagattctt catacgcggc
    39301 acaccgctat agccgcaact gccatgcagt ttatgaatca aatcaaccag gccttccggg
    39361 ttttctccaa ccagctgttc ctcaactttg ttgcgaactt caggcaggaa atcgagtaac
    39421 atttgcagca tatcgcgcgc taaatcggtt tttcctgctg cctggcgtag tgccagttgc
    39481 cagtcgaggg tcgcattcgg gttcaccaca atttcgttga cttcgggcgt cacgacgcga
    39541 gaggaaatac cgctgccagg cttgtagcgc aacagcaaat tatgcaatcg ttcttcttca
    39601 atcggtttcg ccagataatc gctcatccct gcgccaagca gcttctcttt ttgcccggcc
    39661 attgcatgcg ccgttaccgc gataaccggc gtttgttgct gatgggggag ctggtggatg
    39721 agttcgcagg cccgaatacc gtccatgtcc ggcatttgaa tatccattaa gatcaaatcg
    39781 aacggcatct gtttcgcccg ttcaaccgcc tgatgcccgc tatcgcaaag ttccacatgt
    39841 tgcaccatat cttccagcaa tgcgccgata agtttcaggt tagcggggtt gtcatcaacc
    39901 gccatgactg tcattgccag cttactttca tcggttacag gcaaaagcgt gttttgtttg
    39961 tgatgacaaa attccgtcag ggcaggtaac aggcgcgtag gtgttaatgg tttcagcaga
    40021 cacgcgccga taccatcttg cttaagtttt tcagcattga cttgtgcatg gcaaggaagt
    40081 gccagcatga gaaaatcggt catcgatact gctttcgcta atcgctcatg ttgcattgtt
    40141 agcggctcgc ggaaggtcac cgcgatgcct aacaacatca tgtcgtaatg cgcgggaggc
    40201 agcgcggaga acgttgggct ataaaccact tccagcggcg tttcacttaa aatatccagc
    40261 gtgcattgcg ctgctgcgga gtttggttcg acataggcca ggcgcttgcc ggcgaggcac
    40321 tgggtggatg gcccttcgat aataatgttc ggattcagat cgagattaat gtggaaccag
    40381 aaagttgaac cgcgattcgg ctggctatgg aacgaaatat cgccgcccat ttcattaacc
    40441 agtttttgtg taatcaccag ccccagcccg gtaccaccat gacggcggga aatactggca
    40501 tcagcctgtc ggaaggcctg gaataagcgc gattgatcgc gttcaggaat accaatgccg
    40561 gtatcccgaa tctgcacttc aatctgcact ttggtattac tcagcgcacg tttttctacc
    40621 agaatatcaa tgttgccatt ctcggtgaat ttaattgcat tccccaccag gttagtgatg
    40681 atttgctgta atcgcagtgg gtcgccgatc acgttatcgg gcacgtcgct cttaatattg
    40741 agcgtcagtt ccaacccttt atcgtgagaa gaatgtgcca gcagggtaac gacttcatcc
    40801 agcgtgctgc gtagtgggaa tggaatactt tccagaatca gcttacctgc ttccagtttc
    40861 gagaagtcga gaacatcatt aataattgcc agcaaattat ttgccgaacg ttcaatcgta
    40921 ttcaggtgat cgcgctgcgt tggtgttaat tctgttttca gcgtcaggcg ggtaaagcca
    40981 ataacaccat tcagtggtgt acgcagctcg tgtgacatat ttgccagaaa ctcggattta
    41041 atacgcgccg cttcctgggc gcgctttttc gccagatcca actcaacgtt ctgaatttcc
    41101 atctgctcca gcgtttcacg cagatcggac gtcgcctggt cgatattatg ctgcatctct
    41161 tcgtgataag cagccagcga cattgccatc gagttgatac cgtttttcag catatccagc
    41221 tcgccgagca taaatccttc cacccggctg tcgagttgcc cgcgacggat gcggtcgacg
    41281 gtattcacca tgttgcgaat cggaccggtt acatcgcgca ttaagcgcca gccaaaaata
    41341 agcgcaatac cgatacaaaa cagcatcatc acgctggaaa taaagatctc tttatattgc
    41401 tgcaagcgaa ccgatttaag atccagctcc agcgcaatat atcccagcat attttgacta
    41461 tttttggcat cgctactggg cgattcgtcg ggggagtaac tctcagaaat aatcggcgtg
    41521 cggaggatca taatatcgcc gtcacgagtg acagtgagct ggcgaggaaa cggcacgttg
    41581 ctgccgagct gcattgatga aggatcaaga tgaaaattgg aggtgacaaa gagtcggtta
    41641 ttttcatcat aaaccgaaat cgcgcgaaca atatcggaat ggcgacgatg cagtacgctt
    41701 attaactgac cgatagattc gcgattttgc aggctcatgc catattcagt agaaactgca
    41761 agcggctcaa taatgctggc tccggcatct tccagttgac gctgcaagtc gttatagcga
    41821 tgcacaacga aaaagatact cagcaataaa ccaataagga cggtcggtgc caggatcaga
    41881 atcatcatgc gtgcgcgcag gctgtagttg gtcatggagt tccgttatgg gacaattaag
    41941 gtcacactgt taaattgaga aagtctcgcc aatggcgcaa ttctactctg caaaacgacg
    42001 cacgacgacg cgtcagatca taactgtttc agtcaacgac ctcgactctt ttggtcaggg
    42061 cgtggcgcga cataacggca aagcgttatt tattcctgga ttactgccac aggaaaacgc
    42121 ggaagttact gttactgaag ataaaaaaca gtacgctcgc gctaaagtcg tgcgccggtt
    42181 aagcgataac ccggaacgcg aaacgccacg ctgccctcat tttggcgtat gcggcggctg
    42241 tcagcaacaa cacgccagcg tggatttaca gcagcgaagc aaaagtgcgg cactcgcccg
    42301 attaatgaaa cacgaagtct ctgaagtgat cgccgatgtt ccctggggct atcgccgtcg
    42361 cgcgcgttta agtttgaact acttaccgaa aacacagcaa cttcagatgg ggtttcgcaa
    42421 agcgggctcc agtgacattg tcgacgttaa acaatgcccc attttagtgc cccaacttga
    42481 agcattgctg cccaaagtca gggcatgcct ggacagctta caagctatgc gccatcttgg
    42541 tcatgttgaa ctggtacagg caaccagcgg cacgctgatg attttgcgcc ataccgcacc
    42601 gctaagttcg gctgatcgcg aaaaactgga acgcttttcg cattctgaag gcctggatct
    42661 gtatctcgcc cccgatagtg agatactcga aaccgtcgct ggtgagatgc cctggtatga
    42721 ctcaaacggg ttgcgcttaa cttttagccc gcgcgatttt attcaggtca atgcgggtgt
    42781 gaaccaaaaa atggtagcgc gtgcgttgga atggctggat gtgcaacctg aagatcgcgt
    42841 actggatctg ttctgcggta tgggcaactt tacactgcca ttggcgacac aagctgccag
    42901 tgtggtgggt gtagaaggcg ttccggcgct ggtggaaaaa ggccagcaga atgcgcgtct
    42961 taatggctta cacaatgtga cgttttatca cgaaaatctt gaagaagatg tcacaaagca
    43021 gccgtgggcg aaaaacggct tcgataaagt gttgctggac ccggcgcgag caggtgccgc
    43081 aggtgttatg cagcaaatta taaaactgga acctattcgt atagtttatg tatcctgtaa
    43141 ccctgcaacg ctggctcggg atagcgaagc gttattaaaa gcaggatata ccattgcgcg
    43201 actggcgatg ctggatatgt tcccacacac gggacatctg gaatcgatgg tacttttctc
    43261 gcgcgttaaa tagttacgat ttgccgattt cggcaggtct ggtccctaaa ggagaggacg
    43321 atggttgcgg taagaagtgc acatatcaat aaggctggtg aatttgatcc ggaaaaatgg
    43381 atcgcaagtc tgggtattac cagccagaag tcgtgtgagt gcttagccga aacctgggcg
    43441 tattgtctgc aacagacgca gggacatccg gatgccagtc tgttattgtg gcgtggtgtt
    43501 gagatggtgg agatcctctc gacattaagt atggacattg acacgctgcg ggcggcgctg
    43561 ctgttccctc tggcggatgc caacgtagtc agcgaagatg tgctgcgtga gtgcgtcggt
    43621 aagtcggtcg ttaaccttat tcacggcgtg cgtgatatgg cggcgatccg ccagctgaaa
    43681 gcgacgcata ccgattctgt ttcctccgaa caggtcgata acgttcgccg gatgttattg
    43741 gcgatggtcg atgattttcg ctgcgtggtc attaaactgg cagagcgtat tgctcatctg
    43801 cgcgaagtaa aagacgcgcc ggaagatgaa cgcgtactgg cggcaaaaga gtgcaccaat
    43861 atctacgcgc cgttggcgaa ccgtcttggg attggacaac tgaaatggga gctggaagat
    43921 tactgcttcc gttatctcca cccgaccgaa tacaaacgca tcgcaaaact gttgcatgaa
    43981 cgccgtctcg accgcgaaca ctatatcgaa gagtttgtcg gtcatctgcg cgctgagatg
    44041 aaagctgaag gcgttaaagc tgaagtgtat ggtcgtccga aacacatcta cagcatctgg
    44101 cgcaaaatgc agaaaaagaa cctcgccttc gatgagctgt ttgatgtgcg tgcggtacgt
    44161 attgtcgccg agcgtttaca ggattgttat gccgcactgg ggatagtgca cactcactat
    44221 cgccacctgc cggatgagtt tgacgattac gtcgctaacc cgaaaccaaa cggttatcag
    44281 tctattcata ccgtggttct gggaccaggt ggcaaaaccg tagagatcca aatccgcacc
    44341 aaacagatgc atgaagatgc agagttgggt gttgctgcgc actggaaata taaagaaggc
    44401 gcggcagctg gcggcgcacg ttcgggacat gaagaccgaa ttgcctggct gcgtaaactg
    44461 attgcgtggc aggaagagat ggctgattcc ggcgaaatgc tcgacgaagt acgcagccag
    44521 gtctttgacg accgggtgta cgtctttacg ccgaaaggtg acgtcgttga tttgcctgcg
    44581 ggatcaacac cgctggactt cgcttaccac atccacagtg atgtcggaca ccgctgcatc
    44641 ggggcaaaaa ttggcgggcg cattgtgccg ttcacctacc agctgcagat gggcgaccag
    44701 attgaaatta tcacccagaa acagccgaac cccagccgtg actggttaaa cccaaacctc
    44761 ggttacgtca caaccagccg tgggcgttcg aaaattcacg cctggttccg taaacaggac
    44821 cgtgacaaaa acattctggc tgggcggcaa atccttgacg acgagctgga acatctgggg
    44881 atcagcctga aagaagcaga aaaacatctg ctgccgcgtt acaacttcaa tgatgtcgac
    44941 gagttgctgg cggcaattgg tggcggggat atccgtctca atcagatggt gaacttcctg
    45001 caatcgcaat ttaataagcc gagtgccgaa gagcaggatg ccgccgcgct gaagcaactt
    45061 cagcaaaaaa gctatacgcc gcaaaaccgc agtaaagata acggtcgtgt ggtggtagaa
    45121 ggtgtcggca acctgatgca ccacatcgcg cgctgctgtc agccgattcc tggagatgag
    45181 attgtcggct tcattaccca gggacgcggt atttcagtac accgcgccga ttgcgaacaa
    45241 ctggcggaac tgcgctccca tgcgccagaa cgcattgttg acgcggtatg gggtgagagc
    45301 tactccgccg gatattcgct ggtggtccgg gtgatggcca atgatcgcag tggattattg
    45361 cgtgatatca cgaccattct tgccaacgag aaggtgaacg tgcttggcgt tgccagccgt
    45421 agcgacacta aacagcaact ggcgactatc gacatgacca ttgagattta caatctgcaa
    45481 gtgctgggcc gcgtgctggg taaactcaat caggtgccag acgttatcga cgcgcgtcgg
    45541 ttgcacggga gttaggccga aattagctcg tatctacaat gtagattgat atatactgta
    45601 tctacatatg atagcggttt gaggaaaggg ttatgatcca cagtagcgta aagcgttggg
    45661 gaaattcacc ggcggtgcgg atcccggcta cgttaatgca ggcgctcaat ctgaatattg
    45721 atgatgaagt gaagattgac ctggtggatg gcaaattaat tattgagcca gtgcgtaaag
    45781 agcccgtatt tacgcttgct gaactggtca acgacatcac gccggaaaac ctccacgaga
    45841 atatcgactg gggagagccg aaagataagg aagtctggta atggtaagcc gatacgtacc
    45901 cgatatgggc gatctgattt gggttgattt tgacccgaca aaaggtagcg agcaagccgg
    45961 acatcgtccg gctgttgtcc tgagtccgtt catgtacaac aacaaaacag gtatgtgtct
    46021 gtgtgttcct tgtacaacgc aatcaaaagg atatccgttc gaagttgttt tatccggtca
    46081 ggaacgtgat ggcgtagcgt tagctgatca ggtaaaaagt atcgcctggc gggcaagagg
    46141 agcaacgaag aaaggaacgg ttgccccaga ggaattacag ctcattaaag ccaaaattaa
    46201 cgtgctgatt gggtaggtgt tactaactca cttctattct ggtcacaggt ttccacctga
    46261 cggacccgca attttcagga cattctaatg aatcaaatcg accgtttgct caccatcatg
    46321 cagcgcctgc gcgatccgga aaacggctgc ccgtgggata aagagcagac atttgccacc
    46381 attgcgcctt acacccttga agaaacctac gaggtgctgg acgccatcgc ccgtgaagat
    46441 tttgacgatc ttcgcggtga actgggcgat ctgctgttcc aggtggtgtt ttacgcacaa
    46501 atggctcagg aagaagggcg ctttgacttt aatgatatat gcgccgcgat cagcgataaa
    46561 ttagaacgtc gccatccgca tgtttttgct gacagttctg ccgaaaacag tagtgaagtg
    46621 cttgcccgtt gggagcaaat caaaaccgaa gagcgcgcgc agaaagcgca gcattcggcg
    46681 ctggacgata ttcctcgtag tttaccggct ttaatgcgtg cgcaaaaaat ccagaaacgt
    46741 tgcgccaacg ttggttttga ctggacgacg cttggtccgg tagtcgataa agtctacgaa
    46801 gagatcgacg aggtgatgta cgaggcacgg caggctgtta tcgaccaggc taaactggag
    46861 gaggaaatgg gcgacctgct gtttgccacg gtcaatctgg ctcgtcattt agggacaaaa
    46921 gcggaaatcg cattgcaaaa agcgaacgaa aaattcgagc gtcgttttcg cgaagtggag
    46981 cgtattgttg ccgcgcgtgg actggaaatg acaggtgttg acctcgaaac aatggaagaa
    47041 gtctggcaac aggtaaaacg gcaggaaatt gatctctaag ggaattacgt ggtcaagcgc
    47101 gatttgtgtc attttttaaa tgacaagcgc ttgatttgcg tcaaaaacat ttaccccaaa
    47161 ggggctattt tctcactcct gatttcaata gtgcgctggc gaagaggagg gataatgaaa
    47221 gtttgtggca taggtcatgt tcgggtatac tgctttcccg tcctggttat tccatcgtct
    47281 tttcaaccta acttctcagg ttcagcatga caacgaacta tatttttgtg accggcgggg
    47341 tcgtatcctc tctgggtaaa ggcattgccg cagcctccct cgcagccatt cttgaagccc
    47401 gtggcctcaa tgtgaccatc atgaaactgg atccgtacat caacgtcgat ccaggtacta
    47461 tgagcccaat ccaacacggg gaagtgttcg ttaccgaaga cggcgctgaa accgacctgg
    47521 acctggggca ctacgagcgt ttcattcgta ccaaaatgag ccgccgcaac aacttcacca
    47581 cgggtcgtat ctactctgac gttctgcgta aagaacgccg cggtgactac ctcggcgcaa
    47641 ccgtacaggt tattccgcac atcactaacg ctatcaaaga gcgcgtgctg gaaggcggcg
    47701 aaggtcatga cgtggtactg gtcgaaatcg gcggtacagt aggtgatatc gaatccctgc
    47761 cgttcctcga agcgattcgc cagatggctg ttgaaattgg ccgtgagcac accctgttta
    47821 tgcacctgac gctggtgccg tacatggcag cgtctggtga agtcaaaacc aaaccgactc
    47881 agcactctgt aaaagagctg ctctccatcg gtatccagcc tgacatcctg atttgtcgtt
    47941 cagatcgcgc cgttccggcg aacgaacgtg cgaagattgc attgttctgt aatgttccgg
    48001 aaaaagcggt tatttctctg aaagacgtcg attccatcta taaaattccg ggcctgttga
    48061 aatcgcaggg gctggacgat tatatttgta aacgattcag cttaaactgc ccggaagcga
    48121 atctgtccga atgggaacag gttatcttcg aagaagcgaa tccggtaagt gaagtcacca
    48181 ttggtatggt cggcaagtac attgaactgc cggacgctta caaatccgtg atcgaagcac
    48241 tgaaacacgg tgggctgaag aatcgtgtca gcgtcaacat caaactgatc gattcacaag
    48301 atgttgaaac gcgcggcgtt gaaattctta aaggtctgga cgcaatcctc gtacctggcg
    48361 gtttcggcta tcgtggcgta gaaggcatga ttacgaccgc gcgttttgcg cgtgagaaca
    48421 atattcctta tctgggcatt tgcctgggta tgcaggtggc gttaattgat tacgctcgcc
    48481 atgttgccaa catggagaac gccaactcta cggaatttgt gccagactgt aagtacccgg
    48541 ttgtggcgct gattaccgag tggcgcgatg aaaacggcaa cgttgaagtt cgtagcgaga
    48601 agagcgatct cggcggtacc atgcgtctcg gcgcacagca gtgccagttg gttgacgata
    48661 gcttggttcg ccagctgtac aatgcgccga caattgttga gcgtcatcgt caccgttacg
    48721 aagtcaacaa catgctgttg aaacagattg aagatgcagg tctgcgcgtt gcgggccgtt
    48781 ccggggatga tcagttggtc gagatcatcg aagttccgaa tcacccgtgg ttcgtggctt
    48841 gccagttcca tccggagttt acttctactc cacgtgatgg tcacccgctg tttgcaggct
    48901 ttgtgaaagc cgccagcgag ttccagaagc gtcaggcgaa gtaagaaaaa agttagaacg
    48961 gcaacgcgta ccctgggtac gcgttgtttg tctggagttt cagtttaact agtgacttga
    49021 ggaaaaccta atgtccaaaa tcgtaaaaat catcggtcgt gaaatcatcg actcccgtgg
    49081 taacccgact gttgaagccg aagtacatct ggagggtggt ttcgtcggta tggcagctgc
    49141 tccgtcaggt gcttctactg gttcccgtga agctctggaa ctgcgcgatg gcgacaaatc
    49201 ccgtttcctg ggtaaaggcg taaccaaagc tgttgctgcg gtaaacggcc cgatcgctca
    49261 ggcactgatt ggcaaagatg ccaaagatca ggctggcatt gacaagatca tgatcgacct
    49321 ggacggcacc gaaaacaaat ccaaattcgg cgcgaacgca atcctggctg tatctctggc
    49381 taacgccaaa gctgctgctg ccgctaaagg catgccgctg tacgagcaca tcgctgaact
    49441 gaacggcact ccgggcaaat actccatgcc ggttccgatg atgaacatca tcaacggtgg
    49501 tgagcacgct gacaacaacg ttgatatcca ggaattcatg attcagccgg ttggcgcgaa
    49561 aactgtgaaa gaagccatcc gcatgggttc tgaagttttc catcacctgg caaaagttct
    49621 gaaagcgaaa ggcatgaaca ctgctgttgg tgacgaaggt ggctatgcgc cgaacctggg
    49681 ttccaacgct gaagctctgg ctgttatcgc tgaagctgtt aaagctgctg gttatgaact
    49741 gggcaaagac atcactctgg cgatggactg cgcagcttct gaattctaca aagacggtaa
    49801 atacgttctg gctggcgaag gcaacaaagc attcacctct gaagaattca ctcacttcct
    49861 ggaagaactg accaaacagt atccgatcgt atctatcgaa gacggtctgg acgaatctga
    49921 ctgggacggt ttcgcatacc agaccaaagt tctgggcgac aaaatccagc tggttggtga
    49981 cgacctgttc gtaaccaaca ccaagatcct gaaagaaggt atcgaaaaag gtatcgctaa
    50041 ctccatcctg atcaaattca accagatcgg ttctctgacc gaaactctgg ctgcaatcaa
    50101 gatggcgaaa gatgctggct acactgcagt tatctctcac cgttctggcg aaactgaaga
    50161 tgctaccatc gctgacctgg ctgttggtac tgctgcaggc cagatcaaaa ctggttctat
    50221 gagccgttct gaccgtgttg ctaaatacaa ccagctgatt cgtatcgaag aagctctggg
    50281 cgaaaaagca ccgtacaacg gtcgtaaaga gatcaaaggc caggcataag tctgacttta
    50341 tctgatttga taatgccagc cttcgggctg gcatttttta tggtggagtt accagcgacc
    50401 ggatgcgccg ccaccgccag aagaaccgcc accatcgctg aagccgccgc tatctgagcc
    50461 tccagcggaa ccgctgttgt ctgaatgata acttctacca gatgctcttt tctgaagtac
    50521 acataaacag gtaaagacca taatggtggt gaaagtaaaa aagaagaaaa atacagcaat
    50581 ggaaaaagtt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
    50641 actgctttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
    50701 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
    50761 aatttctgaa ggattagccg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
    50821 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
    50881 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
    50941 gatgcggaca gtacgatccg accaggcaac aatgatcagt atcccatcat tacgtttggc
    51001 atctcctaaa cgccaattgt caaaaacccg tgtagcatat ttttcaattg tctcgtcttt
    51061 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
    51121 cttgtacgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
    51181 ttgctgtaat gatgggacaa tagttggttg ggctgcaata aaggaacaca cgaatgtgaa
    51241 tattagtatg aaatagcgca ttataaatgt cccattaaag ctggataaat aaattgacgg
    51301 cggcatcggt aatttgagat tcgttataat taaaaacgaa cacagaaaaa ataagtataa
    51361 ttttggtgaa tgtttttgac atgattaaat ccctttactt tatttatttc tgacataata
    51421 cgttactccc ttttattatt aacgatctgt ttctcatttt tctttaaacc tgcctgtaac
    51481 agaatgttaa taagacatga ttgttgtggc aggatctgca ataattaaca attatccact
    51541 tcacagagaa tgctatgcag tacccgatta acgagatgtt ccagaccctg caaggtgagg
    51601 gttactttac cggcgtcccc gccattttta ttcgtttaca gggatgcccg gttggctgtg
    51661 cctggtgcga caccaaacac acctgggaaa agcttgagga tcgggaagtc tcccttttca
    51721 gcattctggc gaagactaaa gagagtgata agtggggggc ggcgagcagt gaagacttgc
    51781 tggctgtcat tggtcgccag ggatacaccg cgcggcatgt ggtgattacg ggtggtgagc
    51841 cttgcattca tgatttgctg ccactgactg acctgctcga aaagaacggt tttagctgcc
    51901 agatagaaac cagcggtact catgaagtgc gttgtacgcc gaatacctgg gtcaccgtgt
    51961 cgccaaagct aaatatgcgt ggtggttatg aagtattgtc gcaagccctg gagcgagcta
    52021 acgaaatcaa acacccagtt ggacgcgtac gtgatatcga agcactggat gaattgcttg
    52081 cgacgctgac cgatgataaa ccgcgagtca ttgcactgca gccgattagc caaaaggatg
    52141 atgccacacg tttgtgcatt gaaacctgca ttgcgcgtaa ttggcgtttg tcgatgcaaa
    52201 cacataaata tttaaatatt gcctgattac acatttataa gcgttataaa tgggtggaac
    52261 ctgtaaagac ttctactcat ttatattctt tgtcgcctct aaaaaacctc cattttgccc
    52321 atcctggact aatcattatc attttctaca aattctgtgg cgttaatttt tcgttggagt
    52381 gaaaattatt acgtcggagt ttggtggatt ttagtcggta tagaattact ttaaatattt
    52441 ggcttttcaa tcaatgaatt aagtgctctt taacataatg gatgtgttgt ttgtgtgttt
    52501 ctgttaagtt ggtagattgt gactgactta aaaaatcaat aattaataat aggttatgtt
    52561 tagtgagttc cccgcgccag cggggataaa ccgacattga ctgcccgtgg cgtccccgcg
    52621 accttgagtt ccccgcgcca gcggggataa accgagtttg tcgaacacgg agcggagcat
    52681 acccgcgagt tccccgcgcc agcggggata aaccgtgcac gccctgccgg aatctcccct
    52741 cgctctcgag ttccccgcgc cagcggggat aaaccgagta agccggttga ttatcgccag
    52801 ggatattaga gttccccgcg ccagcgggga taaaccgcgt ttagctccgc aatgttggat
    52861 atcactgatg agttccccgc accagcggga ataaaccacc atataacccg ttatctcttt
    52921 ctcaagtttt tatattagca gtactcgtat taagcaacat atccacgtaa cacctcatgt
    52981 tcaaaatagc tctccatata tgagaagttc acaattatcg atacaaaaaa tcaaatttaa
    53041 tcaaagtgtt atttgtataa tccttaaacc gttaagaaat tttaacatat tattttttta
    53101 atattaatta atgcctgtta attttttctt tagaataaca gtgtattttt taagcttgtt
    53161 attcattgat taagtaataa atctggaaat ttgtctttgt tttgaggcta atgagtggtt
    53221 ttacataatc gcctctatac gctgttgatg aatagttctt atgaataaag atatccagtt
    53281 catactttaa gtgaaaattg ataaagtgcg attcgtattg tcttttattc taaagacatc
    53341 gagtgtagtt aatattcctt gtaaaaacag ggataaaccg aactagttaa agtttttatt
    53401 atgttccctg catcagcggg gataaaccgg acttgttatc attttttaag ttgtcgaact
    53461 tatgttcccc gcgccagcgg ggataaccat tcaggtatgt tcaggccggg cagtttcccg
    53521 cccggtcttc ctcacttacg cattcgacca gctctggatc gaatccgcat tccccaattc
    53581 cccatgcatc acttcatacg attttttcag gattacatca gtatgtttgg ttaactcctt
    53641 gaaaatgcct ttattcatcg cgttgtagcg tttggcgttg ctctcaacag gcataaagat
    53701 atctttcacg cgaaccattt tgtcgacagc cgttgcgtaa tccgggtata gccccagacc
    53761 taccgccgta ttaatcgctg cccccaggct tgcacaacca ttaatggcgt tacggcgtgc
    53821 cggaaggttg aacacgtcgg caaaaatctg cataaacaga tcgctgttcg aaccgccgcc
    53881 agtaatgatc acatgctttg caaagtgatt catttcatta cacatattgt cgtaattgtt
    53941 cttcagcgtc agcgccacgc tttccagtat cgaacgatat atccatgcgt aatccatgct
    54001 ggaatcaaag ccaatcataa tcccgcgttt gtacggttcc cacggattgg tcagccagtc
    54061 cagcaccgtc atcagaccat tacagcctgg cggcacacaa gacgcttttt tgttgagcaa
    54121 atcttccggt gaaagatcct gtgctttggc atcctgaatt aacgactcgc ctaacatgtc
    54181 gcgtagccag ctcaccgtcc acatgccttt gcgaataccg taaccttcat acagcaaggt
    54241 ttgcggaata gaagacataa tcggccagta cgctaccgga tctttcggta gtaatttgcc
    54301 gttcatcatc aacgcgatgt aagtgccaag agaaattacc gcagtttcat catccagtaa
    54361 cccggctccc agtgcttcta ccggtttatc actggtggta caaacaaccg gcagcccagc
    54421 cgggaaatgt gtcgccagag cggcttgagg agtgatatgc ccaagaacag tgcccggcat
    54481 ttgcacatca aacagcatat gacgggggat attaaacttc tccatgactg cggcatcttc
    54541 actccatgcc caggtcttat aatccaccgg ccactgacca aaatagttgg cgatattgtc
    54601 tttaaactcg ccggttaagc gatgcgtcag ataacccgaa aaagaggtga catatgccac
    54661 gtcaggattc gtatgttcgt aagggcgtgt aacgcgtgca tcctgccagc taatcaacgg
    54721 cgcagctggc gtgccatcgg ctttcaataa cgcacggcag caacggatgg atcccagacc
    54781 aataccgaca atatcttctt tatttccggc aaactgactc atcaaatcgt gaccggcaaa
    54841 gcataatgat gcccataaat cgtcgtcagg atgttctgcg gtatcggcat ctggcgtgtg
    54901 catcggctgc aataagcctt tgccttcgca aaccacatta ccttccagat cgtacatcac
    54961 cacttttgtg ctctgacttc caccatcaat ccctatgatg tattttttcg acatgatttt
    55021 tctcctgtaa ttttctggac ttacttgctg ctagccatag caggggcgct atcagcagta
    55081 cgaatttttt ggaacagcaa aaaggtaaag acgatcacca tggcaagcgc cgccatgccc
    55141 atcagccaca tattgcggta ggcttctgca gcgggcaggg tgtcttgcca gtagccgata
    55201 atcgggtaaa cgaagacatc cggcaggaat ccaatcaccg aacaaatacc taccgtggtg
    55261 cccataatgt aagacggtgt tctcgcttcg ccagggcagg cccagtacag cccgcgtgag
    55321 gcgtaacagg tgaatcccag cagtaaaatc aggccaatcc ccatcgcgac cgattgcggg
    55381 ttagagttcg tgacgagcag ggcagttaac gccagcaagc cgagtacgga aaggatttgg
    55441 atcacgcggg taggggattt cactttgctg taggtggtga taatgccgcc aagtgggccg
    55501 cacagcgcgc ggaagatttt gttgatcaca atccccatgt agctcgccgc caccagcgac
    55561 atgccgtaca tttcggtcag atagttggtg gagtaactca gaatggcgta gatggtgaag
    55621 acgccaaaaa tcaccatgct gcaataccag gtggtgctga tgcgcaaaac ggccaggatg
    55681 tcgctaagct ggaatgactg tttttcttcg ttattggcac tgcgcagatt gttgttatcg
    55741 ctaacaaaaa accagcacag aatccccaac aagatgtaaa ccacactata gatgatgatg
    55801 accgttttca gactggcgct gtcatccgga gcgaagcgag aaaagaccca catggtaaac
    55861 accgccagcg acattacacc gacgccgcgc agcccttcca tccagcccat aattttccct
    55921 tgctcgctat gatcgcccag caatgaggcg gctttaatcg acaccgacca cagcattaaa
    55981 atagtggtta tcgcaaaggc gacctgaata cagagcatta cccacagcgg tggatatgtt
    56041 gccattaaca gacccagtaa tccggtaatg atcatcgcgg aagtaatcat tttgcggtgt
    56101 gaaaatttat cggcaataac gccgctgggg gcataaagaa taatggccgc aataccaaat
    56161 gtactcatta ttaaacctat ctcggtattg ctgaatccca taaatttcgc catgggaatt
    56221 tgataaatat aacgtaaata agccaggtcg aaactaacgc cgccgctaaa actaattatc
    56281 gcgagggcta tccaacggcg atatgagttg tgttgcatat cgtattccca tttgtaatag
    56341 acaaaaaaaa gagaaaagta agccgccctt tcaggtggat tacttttctc aggtctctag
    56401 taattgcaat aagaaataac atggaaagca gtggtgttgt gtgagagtaa tcacgcttct
    56461 gctttttaaa attatttgac aattcgaata tcacactgtg attacgatca cattagcgat
    56521 tatgccctta tgataaaaat tatctccagt taccagagat aatgagaaag gtaacttccc
    56581 tctcgcgggg g
//
LOCUS       DAPOZO010000023        56270 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPOZO010000023 DAPOZO010000000
VERSION     DAPOZO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.023"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1222)
                     /gene="fabF"
                     /locus_tag="ROI21_001480"
     CDS             complement(<1..1222)
                     /gene="fabF"
                     /locus_tag="ROI21_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDX5110419.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGS"
     gene            complement(1310..1546)
                     /gene="acpP"
                     /locus_tag="ROI21_001481"
     CDS             complement(1310..1546)
                     /gene="acpP"
                     /locus_tag="ROI21_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDX5110420.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(1757..2491)
                     /gene="fabG"
                     /locus_tag="ROI21_001482"
     CDS             complement(1757..2491)
                     /gene="fabG"
                     /locus_tag="ROI21_001482"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDX5110421.1"
                     /translation="MSFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(2504..3433)
                     /gene="fabD"
                     /locus_tag="ROI21_001483"
     CDS             complement(2504..3433)
                     /gene="fabD"
                     /locus_tag="ROI21_001483"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDX5110422.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRAWQQQGGKAPALMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(3449..4402)
                     /gene="fabH"
                     /locus_tag="ROI21_001484"
     CDS             complement(3449..4402)
                     /gene="fabH"
                     /locus_tag="ROI21_001484"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDX5110423.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLTANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(4470..5540)
                     /gene="plsX"
                     /locus_tag="ROI21_001485"
     CDS             complement(4470..5540)
                     /gene="plsX"
                     /locus_tag="ROI21_001485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDX5110424.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(5621..5794)
                     /gene="rpmF"
                     /locus_tag="ROI21_001486"
     CDS             complement(5621..5794)
                     /gene="rpmF"
                     /locus_tag="ROI21_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDX5110425.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(5846..6367)
                     /gene="yceD"
                     /locus_tag="ROI21_001487"
     CDS             complement(5846..6367)
                     /gene="yceD"
                     /locus_tag="ROI21_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDX5110426.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            6566..7150
                     /gene="yceF"
                     /locus_tag="ROI21_001488"
     CDS             6566..7150
                     /gene="yceF"
                     /locus_tag="ROI21_001488"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDX5110427.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(7262..8221)
                     /gene="rluC"
                     /locus_tag="ROI21_001489"
     CDS             complement(7262..8221)
                     /gene="rluC"
                     /locus_tag="ROI21_001489"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDX5110428.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            8794..11979
                     /gene="rne"
                     /locus_tag="ROI21_001490"
     CDS             8794..11979
                     /gene="rne"
                     /locus_tag="ROI21_001490"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDX5110429.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQAAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAIAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVKPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            12112..12405
                     /locus_tag="ROI21_001491"
     CDS             12112..12405
                     /locus_tag="ROI21_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDX5110430.1"
                     /translation="MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            12402..12896
                     /locus_tag="ROI21_001492"
     CDS             12402..12896
                     /locus_tag="ROI21_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDX5110431.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIGR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(12991..13944)
                     /gene="flgL"
                     /locus_tag="ROI21_001493"
     CDS             complement(12991..13944)
                     /gene="flgL"
                     /locus_tag="ROI21_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDX5110432.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(13956..15599)
                     /gene="flgK"
                     /locus_tag="ROI21_001494"
     CDS             complement(13956..15599)
                     /gene="flgK"
                     /locus_tag="ROI21_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDX5110433.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTTGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(15665..16606)
                     /gene="flgJ"
                     /locus_tag="ROI21_001495"
     CDS             complement(15665..16606)
                     /gene="flgJ"
                     /locus_tag="ROI21_001495"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDX5110434.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKTGEDPAANIRPVALQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(16606..17703)
                     /gene="flgI"
                     /locus_tag="ROI21_001496"
     CDS             complement(16606..17703)
                     /gene="flgI"
                     /locus_tag="ROI21_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDX5110435.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(17715..18413)
                     /gene="flgH"
                     /locus_tag="ROI21_001497"
     CDS             complement(17715..18413)
                     /gene="flgH"
                     /locus_tag="ROI21_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDX5110436.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(18466..19248)
                     /gene="flgG"
                     /locus_tag="ROI21_001498"
     CDS             complement(18466..19248)
                     /gene="flgG"
                     /locus_tag="ROI21_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDX5110437.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(19385..20140)
                     /gene="flgF"
                     /locus_tag="ROI21_001499"
     CDS             complement(19385..20140)
                     /gene="flgF"
                     /locus_tag="ROI21_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDX5110438.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQTTRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(20160..21365)
                     /gene="flgE"
                     /locus_tag="ROI21_001500"
     CDS             complement(20160..21365)
                     /gene="flgE"
                     /locus_tag="ROI21_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDX5110439.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLVFNANGVLTSDPTANISTGAINGAVPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(21390..22085)
                     /gene="flgD"
                     /locus_tag="ROI21_001501"
     CDS             complement(21390..22085)
                     /gene="flgD"
                     /locus_tag="ROI21_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDX5110440.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(22097..22501)
                     /gene="flgC"
                     /locus_tag="ROI21_001502"
     CDS             complement(22097..22501)
                     /gene="flgC"
                     /locus_tag="ROI21_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDX5110441.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(22505..22921)
                     /gene="flgB"
                     /locus_tag="ROI21_001503"
     CDS             complement(22505..22921)
                     /gene="flgB"
                     /locus_tag="ROI21_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDX5110442.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            23076..23735
                     /gene="flgA"
                     /locus_tag="ROI21_001504"
     CDS             23076..23735
                     /gene="flgA"
                     /locus_tag="ROI21_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDX5110443.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            23811..24104
                     /gene="flgM"
                     /locus_tag="ROI21_001505"
     CDS             23811..24104
                     /gene="flgM"
                     /locus_tag="ROI21_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDX5110444.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            24109..24525
                     /gene="flgN"
                     /locus_tag="ROI21_001506"
     CDS             24109..24525
                     /gene="flgN"
                     /locus_tag="ROI21_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDX5110445.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(24565..26100)
                     /gene="murJ"
                     /locus_tag="ROI21_001507"
     CDS             complement(24565..26100)
                     /gene="murJ"
                     /locus_tag="ROI21_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDX5110446.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(26210..27133)
                     /locus_tag="ROI21_001508"
     CDS             complement(26210..27133)
                     /locus_tag="ROI21_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDX5110447.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(27135..27782)
                     /locus_tag="ROI21_001509"
     CDS             complement(27135..27782)
                     /locus_tag="ROI21_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDX5110448.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDTSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(27793..28377)
                     /gene="rimJ"
                     /locus_tag="ROI21_001510"
     CDS             complement(27793..28377)
                     /gene="rimJ"
                     /locus_tag="ROI21_001510"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDX5110449.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            28613..29821
                     /gene="mdtH"
                     /locus_tag="ROI21_001511"
     CDS             28613..29821
                     /gene="mdtH"
                     /locus_tag="ROI21_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDX5110450.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            29885..30532
                     /gene="grxB"
                     /locus_tag="ROI21_001512"
     CDS             29885..30532
                     /gene="grxB"
                     /locus_tag="ROI21_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDX5110451.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            30666..31226
                     /locus_tag="ROI21_001513"
     CDS             30666..31226
                     /locus_tag="ROI21_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX5110452.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            31332..32378
                     /gene="pyrC"
                     /locus_tag="ROI21_001514"
     CDS             31332..32378
                     /gene="pyrC"
                     /locus_tag="ROI21_001514"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDX5110453.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRNGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAASIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            32452..32697
                     /gene="dinI"
                     /locus_tag="ROI21_001515"
     CDS             32452..32697
                     /gene="dinI"
                     /locus_tag="ROI21_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDX5110454.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            32987..33241
                     /gene="bssS"
                     /locus_tag="ROI21_001516"
     CDS             32987..33241
                     /gene="bssS"
                     /locus_tag="ROI21_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDX5110455.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            33356..34474
                     /gene="solA"
                     /locus_tag="ROI21_001517"
     CDS             33356..34474
                     /gene="solA"
                     /locus_tag="ROI21_001517"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDX5110456.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATHAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGDYQAKKAIVCAGTWVKDQLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQEKKSDFDLTPFRLSRFQ"
     gene            34522..34635
                     /locus_tag="ROI21_001518"
     CDS             34522..34635
                     /locus_tag="ROI21_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDX5110457.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            34896..35462
                     /locus_tag="ROI21_001519"
     CDS             34896..35462
                     /locus_tag="ROI21_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDX5110458.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            35466..36041
                     /locus_tag="ROI21_001520"
     CDS             35466..36041
                     /locus_tag="ROI21_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDX5110459.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDDLDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(36083..37135)
                     /locus_tag="ROI21_001521"
     CDS             complement(36083..37135)
                     /locus_tag="ROI21_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDX5110460.1"
                     /translation="MPVLHNRISNDALKAKMLADSEPRTTISFYKYFHIADPKATREA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            37360..38280
                     /gene="lpxL"
                     /locus_tag="ROI21_001522"
     CDS             37360..38280
                     /gene="lpxL"
                     /locus_tag="ROI21_001522"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDX5110461.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            38452..39678
                     /gene="mdtG"
                     /locus_tag="ROI21_001523"
     CDS             38452..39678
                     /gene="mdtG"
                     /locus_tag="ROI21_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDX5110462.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            39761..40135
                     /locus_tag="ROI21_001524"
     CDS             39761..40135
                     /locus_tag="ROI21_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HDX5110463.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(40136..40363)
                     /locus_tag="ROI21_001525"
     CDS             complement(40136..40363)
                     /locus_tag="ROI21_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDX5110464.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(40536..43079)
                     /gene="mdoH"
                     /locus_tag="ROI21_001526"
     CDS             complement(40536..43079)
                     /gene="mdoH"
                     /locus_tag="ROI21_001526"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDX5110465.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDADHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(43072..44607)
                     /gene="mdoG"
                     /locus_tag="ROI21_001527"
     CDS             complement(43072..44607)
                     /gene="mdoG"
                     /locus_tag="ROI21_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDX5110466.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLTSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            45001..46158
                     /gene="mdoC"
                     /locus_tag="ROI21_001528"
     CDS             45001..46158
                     /gene="mdoC"
                     /locus_tag="ROI21_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDX5110467.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKTLFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPTR"
     gene            complement(46166..47647)
                     /gene="clsC"
                     /locus_tag="ROI21_001529"
     CDS             complement(46166..47647)
                     /gene="clsC"
                     /locus_tag="ROI21_001529"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDX5110468.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(47589..48122)
                     /gene="ymdB"
                     /locus_tag="ROI21_001530"
     CDS             complement(47589..48122)
                     /gene="ymdB"
                     /locus_tag="ROI21_001530"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDX5110469.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(48217..48528)
                     /locus_tag="ROI21_001531"
     CDS             complement(48217..48528)
                     /locus_tag="ROI21_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX5110470.1"
                     /translation="MFRPFLDSLMLGSMFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSTPVEMNCPQNGSVPGRTYSSKALMTGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(48649..48981)
                     /gene="csgC"
                     /locus_tag="ROI21_001532"
     CDS             complement(48649..48981)
                     /gene="csgC"
                     /locus_tag="ROI21_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDX5110471.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(49040..49495)
                     /gene="csgA"
                     /locus_tag="ROI21_001533"
     CDS             complement(49040..49495)
                     /gene="csgA"
                     /locus_tag="ROI21_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDX5110472.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(49536..49991)
                     /gene="csgB"
                     /locus_tag="ROI21_001534"
     CDS             complement(49536..49991)
                     /gene="csgB"
                     /locus_tag="ROI21_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDX5110473.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGNANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            50735..51385
                     /gene="csgD"
                     /locus_tag="ROI21_001535"
     CDS             50735..51385
                     /gene="csgD"
                     /locus_tag="ROI21_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDX5110474.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            51390..51779
                     /gene="csgE"
                     /locus_tag="ROI21_001536"
     CDS             51390..51779
                     /gene="csgE"
                     /locus_tag="ROI21_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDX5110475.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            51804..52220
                     /gene="csgF"
                     /locus_tag="ROI21_001537"
     CDS             51804..52220
                     /gene="csgF"
                     /locus_tag="ROI21_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDX5110476.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDYGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRIVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            52247..53080
                     /gene="csgG"
                     /locus_tag="ROI21_001538"
     CDS             52247..53080
                     /gene="csgG"
                     /locus_tag="ROI21_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDX5110477.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(53144..53635)
                     /locus_tag="ROI21_001539"
     CDS             complement(53144..53635)
                     /locus_tag="ROI21_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDX5110478.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSTKTAHREQQIK
                     NKA"
     gene            complement(53737..54291)
                     /gene="ycdY"
                     /locus_tag="ROI21_001540"
     CDS             complement(53737..54291)
                     /gene="ycdY"
                     /locus_tag="ROI21_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDX5110479.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(54315..55052)
                     /gene="ycdX"
                     /locus_tag="ROI21_001541"
     CDS             complement(54315..55052)
                     /gene="ycdX"
                     /locus_tag="ROI21_001541"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDX5110480.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(55107..56045)
                     /gene="ghrA"
                     /locus_tag="ROI21_001542"
     CDS             complement(55107..56045)
                     /gene="ghrA"
                     /locus_tag="ROI21_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDX5110481.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYYREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT        13850 a        14770 c        13721 g        13929 t
ORIGIN      
        1 aagaaccatt agtgccaccg aagccgaagg agttacacag cgtgtattcc attccgctga
       61 cctgacgtgc ttcgtgcggt acgaaatcca gatcgcaacc ttcatccggg ttatccaggt
      121 tgatggttgg cggaacagcc tgatcgcgca gcgccaggat ggagtagata gattctactg
      181 cacccgccgc acctaacagg tgaccggtca tagatttcgt ggagcttacc aacacacggc
      241 ttgcagcttc accgaagatg gttttcaccg cctgcgcttc agctttatcg ccagccggag
      301 tagaagtacc gtgtgcgtta acgtagccaa tctgactcgc ttcaatgcct gcatcacgca
      361 aagcatttgc cattgccaga gctgcgcctg cgccattttc tggcggtgac gtcatatgat
      421 aagcatcgct gctcatacca aagccgacga gttcagcgta aattttcgca ccgcgttttt
      481 tcgcgtgttc gtactcttca agtaccagca taccggcacc atcgcccagt acgaaaccat
      541 cacgctcttt atcccacggg cggctcgccg cttgcgggtt atcattgcgg gtagataatg
      601 cacgtgccgc gccaaaacca ccaacgccca gcggcgtact ggctttctct gcgccacctg
      661 caaccatcac gtcagcatcg ccatacgcga taatacgcgc agcatggcca atgttgtgca
      721 cgccggaagt acaggcggtc gcgatagaga tgcttgggcc acgcaggcca tacatgatag
      781 tcagatgacc tgccaccatg ttcacaatcg ttgacggaac gaagaatggg ctgatcttac
      841 gtggaccacc gttcatcaga gatgtgtggt tttcttcgat cagtccgagg ccgccaatcc
      901 cggagccaat tgcggcacca atgcgggttg cgttctcttc cgttatttca aggccagaat
      961 cctgcatggc ctgaacgcca gcgacaattc catattgaat gaaggcatcc atcttgcgct
     1021 gttctttgcg cgagataatg tcctcacagt taaaatcctt tactaagcca gcaaatttcg
     1081 ttgcataggc gctagtatcg aaatggtcga ttaggctgat gccactctga ccggcaagca
     1141 gagctttcca ggtagactct acggtattgc cgacaggaga caacatgccc agtccggtca
     1201 caactacacg acgcttagac acgtttgtcc tccagggagg gaaaaaatga ttctagtggg
     1261 acaaaaagat aaaactcagg cggtcgaacg accgcctgga gatgttcact tacgcctggt
     1321 ggccgttgat gtaatcaatg gcagcctgaa cggtggtgat tttctcagct tcttcgtccg
     1381 gaatctcagt atcaaactct tcttccagag ccattaccag ctcaacggtg tcaagagaat
     1441 ccgcgcccag gtcttcaacg aaagaagcat tgttggtaac ttcttcctgc ttaacgccca
     1501 gctgttcgcc gataattttc ttaacgcgtt cttcgatagt gctcatactc ttaaatttcc
     1561 tatcaaaact cgctttcgcg atggttttcg tagtgtataa aatgttgaaa aatttgcaac
     1621 taaatcccgg caggtcttac cacgatttta cgttattttg aggccttacc ccccaataac
     1681 gcaaatcatt ttgcactaat tattactcat aaccacgcag actgcgcgca acatgagcat
     1741 tttgtgcaaa tcgcggtcag accatgtaca tcccgccgtt cacatgcaaa gtttcacccg
     1801 tgatgtaagc tgcttcgtcg gatgccagga atgcaaccgc gttggcgatt tcctgtgcgc
     1861 cgccgaggcg acccgcagga acctgcgcca ggatacccgc acgctggtca tcgctcagcg
     1921 cacgtgtcat gtccgtttca ataaagcccg gagcaacaac gtttacagta ataccgcgtg
     1981 acgcaacttc gcgcgccagt gatttactga agccgatcaa gcccgctttc gccgcagcgt
     2041 agttggcctg accgccattt cccatggtac caaccacaga accgatagtg ataatacgac
     2101 catgacgctt tttcatcata gcgcgcatta ccgcttttga cagacggaaa acagatgaaa
     2161 ggttggtttc gataatatcg ttccactctt catctttcat tcgcattaac aggttatcac
     2221 gagtgatacc ggcattattg accaggatat ccacttcacc aaattctgcg cgaatttttt
     2281 ccagaacaga ttcgatagat gccgggtcgg tcacattcaa catcagacct ttgccgttgg
     2341 cacctaaata atcactgatc gcctgagcgc cattttcact ggtcgcagtg ccaataactt
     2401 tcgcgccacg ggctgcgagc gtttcagcaa ttgcgcggcc aattccgcgg cttgcaccgg
     2461 ttaccagtgc gatttttcct tcaaaactca tgattttcct cttttaaagc tcgagcgccg
     2521 ctgccatcgc tgaaggttcg ttcagcgccg aggcggtcag ggtgtcgaca atgcgtttcg
     2581 tcaggccagt aagcactttg cccgggccga cttcatagag atgttctacg ccttgcgctg
     2641 ccatgtattc aacagacttc gtccactgaa ccgggttata caactgacgt accagtgcgt
     2701 cacggatggc atcaccattg gtttcgcatt tcacatcaac gttattcaca acaggaactg
     2761 ttggtgcgtt aaaggtgatt ttcgctaatt ctactgccag tttgtcggct gctggtttca
     2821 tcagcgcaca gtgagacggt acgctcactg gtaacggcag agcgcgtttt gcgcccgcag
     2881 ctttacaggc agcgccagca cgctcaactg cttctttatg accggcaata accacctgtc
     2941 ccggagagtt aaagtttacc ggagaaacga cctgaccttc tgcagcttct tcacacgctt
     3001 tcgcaataga cgcatcatcc agaccgatga ttgccgccat agcgcccgtg ccttccggta
     3061 cggcttcttg catgaacttg ccgcgcatct caaccagacg caccgcatca gcgaaatcaa
     3121 tcacaccagc gcaaaccagc gcggagtatt cccccaggct gtgaccggcc atcaatgccg
     3181 gtgctttacc gccctgctgc tgccatgcgc ggtacagcgc aacagatgca gtcaacagcg
     3241 ctggctgggt ttgccaggtt ttattcagtt cttcagctgg cccctgctgg gtcagcgccc
     3301 acaggtcgta gcccagcgcc gcagaagctt cagcaaacgt ttcttcgaca attggatagc
     3361 tcgccgccat atcagccagc attccaacgg tttgagaacc ctgtccaggg aacacaaatg
     3421 caaattgcgt catgttttaa tccttatcct agaaacgaac cagcgcggag ccccaggtga
     3481 atccaccgcc aaaggcttca agcagaacca actgccccgg cttaatgcgc ccgtcgcgta
     3541 cagcttcatc cagagcgcac gggacagagg ccgcagaggt attaccgtgg cgatccagcg
     3601 tcaccacgac attgtccatc gacatgccga gttttttcgc cgttgcactg ataatacgca
     3661 ggttagcctg atgcggaacc agccagtcca gttgagaacg gtcaagatta ttcgccgtca
     3721 gcgtctcatc aacgatgtgc gccagttctg ttaccgcaac cttgaagact tcgttgcccg
     3781 ccatcgtcag atgaattgaa ttctctggat tcacacggtc agcattaggc agcgtcagca
     3841 actcaccata gctaccgtcg gcatgcagat gggtggagat gattcccggc tcttcagagg
     3901 cagccagcac cgcagcgccc gcgccatcgc caaaaataat aatagtccca cgatctgttg
     3961 gatcgcaggt gcgcgccagt acatcggaac cgacgaccag agcatacttc accgccccag
     4021 atttcacgta ttgatcggct acgcttaatg cataggtgaa acctgcgcag gctgctgcaa
     4081 cgtcaaatgc cgggcaacct ttaatgccca gcatgctttg aatctgacaa gctgcgctcg
     4141 ggaaagcgtg cgtagcagaa gtcgttgcca caacgatcag gccaatctgg tctttctcaa
     4201 tgcccgccat ctcaattgcg cgtgtcgccg cttcaaagcc catggttgaa acggtttcgt
     4261 ttggcgcggc aatgtggcgt tcgcggatac cggtacgagt gacaatccac tcgtcagagg
     4321 tgtccaccat tttttccaaa tcggcgtttg tccgcacttg ttcgggcaga tagctgccag
     4381 taccaataat cttcgtatac atgtacgctc agtcactttt cggttatata ccgtcacttg
     4441 caaactgcga gttcgctggc agcgtcctgc taccgcagag ttccgctttt gccaccgtcc
     4501 agcagctcaa aaccagctgg gtatacagat tccaggcgag cggcaattcg ctgaggaact
     4561 tgtcgctgca ccgcctgcac tgcctgttca atcgcgaccg caaaagctcg ctgattggct
     4621 gcaccatgac tttttatcac cgtgccgcgc aatcctaaca gacaggcgcc gttatactgg
     4681 tcggggttga ggtgactgaa tcgcctcgtc aggctctttt gtagccaacg ctttaataac
     4741 agtagccacc acgaccgttt tttcccttca ccctgagatt tcagcagaga aaggaacatc
     4801 ctgacaacac cttccatcgt ctttaatgtg acatttcctg taaagccgtc acaaaccagc
     4861 acatctgtct tgccagttaa caactcattg gcttcaagat agccgatata attgatagaa
     4921 gggattgttt taagcaccgc tgaggcatcc cgaatactgt cgagaccctt tacttcttct
     4981 tcaccaatat tgagcaacgc cacgcgagga ttgggaattt ccaccacctc ttcagccagg
     5041 actgagccca taatggcaaa ttgcaccaac attgtgctgt cacaatcgac gttggcccct
     5101 aaatcgagga ccaccgtttt gcccttttgc tgatgcggta ataccgtcac cagcgccgga
     5161 cgctcaatcc cctccagggg cttgagtaat aattttgcca gccccatcag cgccccggta
     5221 ttaccggcac tgacacaggc ttgcgctcga ccttctttca ccagctccag ggccacgcgc
     5281 attgaactcc cacgactggc gcggatagct tgcgaaggcc gggcatcact ggcgataact
     5341 gactgcgcag gaataatctg cagacgcgaa cgttgttcaa agtcagcttt agcaagcaat
     5401 ggcgtgatgg cgtcgggatt gccgactaaa agaagagtga gttgcgaatt agaattcagt
     5461 gcctgcaatg ctgcaggcac tgtcacggaa gggccaaaat cccctcccat gacatctaac
     5521 gccagggtta gacgtgtcaa ggtatcgtcg ccgcccggtt tggtctttcc ccagttgcct
     5581 ggggaaaatc ctcactaagc ttcatcacgc agacgcgtga ttacttagcg atgaccttgc
     5641 ggccgcggta gtaaccgtcg gcagtgatgt ggtgacgcag gtgtttttca ccagaagttt
     5701 tgtctacaga caggctggtg actgcggtca gcgcgtcatg ggaacgacgc atgccacgtt
     5761 tggaacgggt tggtttattc tgttgtacgg ccatggacct tactcctcaa ttacggttta
     5821 tccccgatcc aacggggagc accaattact tacgctttaa gctggctaat acggcaaatg
     5881 ggtttggctt ttgcgcttct tcaggcagtt caccaaagac catgtccgct tcggacactt
     5941 cacagtgttc agaatcatgc accggaacta ccggcaaggc gaggatgatt tcatcttcaa
     6001 ccattgcaag cagatcgatt tcaccgaatt cgttaacctc aatcggttca tacgcttccg
     6061 gcagtgcttc agcctgttcg tctgaacgca caggactaaa acaatacgtt gtgtagacct
     6121 gatgagtaaa cggcttcccg caacgctgac actcgagcgt taccgtcacc ttcgcatcgc
     6181 cgtttaacac tgcgagacgt tggttatcga tagcgaacga catggagcat tccacatcac
     6241 tgtccacact gactacggat tcggcgacgc gctcaacctg atcaggagta tagataccct
     6301 ggtaatcaag gcgtttttga gccgtacgaa ccggatcgag agtcaggggt aattttacct
     6361 tttgcatagg gcgcgcatat taactttgta acgtcataga gtcaaagaaa aaggcagcct
     6421 gtggttgcct tttgccaata attcgcacac attgcgggtt actgctttat tttcgttcag
     6481 tgctgataac gaacatcaac ctgcaaaacg aagcgcatag tttaaaatgg ctatcatcaa
     6541 tacgctatat ctgatggaaa aaaatatgcc taaacttatt ttagcctcca cctcgccctg
     6601 gcgtcgcgct cttctggaaa aactgcaaat ctcttttgaa tgtgcagcac cagaggtcga
     6661 cgaaacccca cgcagcgacg aatcaccgcg acagttggtg cttcgactgg cacaagaaaa
     6721 agcgcaatct ctggcgtcac gttatccgga tcatttaatt attggttcag accaggtatg
     6781 tgttcttgat ggcgaaatca ccggtaaacc gttaacggaa gaaaatgccc gtctgcaatt
     6841 acgcaaagcc agcggcaata tcgtcacctt ctatactgga ctggcgttgt ttaattcggc
     6901 gaatgggcat ctacaaacag aagtggagcc ttttgacgtc catttccgtc acctgagcga
     6961 ggcggagatt gataattacg tgcgtaaaga gcatcccctg cactgcgcgg gtagctttaa
     7021 gagtgaagga tttggcatta cgctgtttga gcgtttagag gggcgtgatc ctaacacgct
     7081 ggttggttta ccgctaatcg cgctgtgtca gatgttacgt cgggaaggga aaaacccgct
     7141 gatgggatag ttgtttgcgc cggatgcggt tacttcgcat ccggcaatcg gtgctggcgg
     7201 cctgatgcgg cgtaaacgcc ttatcaggcc aaccgcccat tatcaggttt atatgcttgt
     7261 attagcgcgc gttacgcagc ttttgcaaac aacgcttcaa accttcatcc atcggcgctt
     7321 cgatacgcat cacctcaccg gtccccggat gagtaaactt caacgctgca gcgtgcagga
     7381 acaggcgatt taatcccgtg cctgcttcag tgagctgtct gtcaaattca cggtcaccgt
     7441 agcgatcgtc aaaggcaatc ggatgacccg catactgtgt atgaacacgg atctgatgag
     7501 tacgccctgt taccgggcta caacgcacca gggtggcaaa agcatagcgt tcttccactt
     7561 taaagcgtgt ttccgacggt ttgccttcct gactcacacg cacgatacgt tcgccgcttt
     7621 gcagaatatt tttcaataac ggcgcttgaa cgctcttcac atgcgactgc cactgaccgc
     7681 gcaccagcgc caggtaatct ttttgcatcc ctttttcacg taattgctca tgcagagaac
     7741 gcaacgccga gcgttttttc gctaccagca acacacctga agtgtcccgg tcgagacgat
     7801 gaaccagttc aaggaaccgc gcttccgggc gcaacgcccg caaaccttcg ataacgccga
     7861 agcttaaacc actaccgcca tgtaccgccg taccggaagg tttattcagc accaggatat
     7921 ggtcatcttc atataagatg acgtccgcca gcgccgccac cttttgcaga tgtggcgaaa
     7981 ccgcctcttc ttcccgttca gcaacgcgaa ccggtgggat acgcacctca tcacccgctt
     8041 cgagtttata ttcaggctta atacgttttt tgttcacccg cacttcgcct ttacgcaaaa
     8101 tacggtaaat catacttttt ggtacgcctt tcaattgggt acgcaaaaag ttatcgatac
     8161 gttgccccgc ttcgtcagcg gtgatagcaa caatttttac ggatggagtc tctgttttca
     8221 tggtgggcga ttctaattag tcaacaggat tcgcgccact catttttcta tgcttatatt
     8281 tagttttgca ccttattact tcactacgtg atcactttat tgatggttat taaaccaatc
     8341 accagcaaga agtgaaaaaa ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc
     8401 tataacaagg cttgcagtgg aataatgagg ccgtttccgt gtccatcctt gttaaaacaa
     8461 gaaattttac ggaataaccc attttgcccg accgatcatc cacgcagcaa tggcgtaaga
     8521 cgtattgatc tttcaggcag ttagcgggct gcgggttgca gtccttaccg gtagatggaa
     8581 atatttctgg agagtaatac ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg
     8641 aaaaacgggc aaccgacact ctgcgcctct ttgagctgac gataaccgtg aggttggcga
     8701 cgcgactaga cacgaggcca tcggttcaca cccggaaagg cgttactttg cccgcagctt
     8761 agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat caacgcaact
     8821 cagcaggaag agttgcgcgt tgcccttgta gatgggcagc gtctgtatga cctggatatc
     8881 gaaagtccag ggcacgagca gaaaaaggca aacatctaca aaggtaaaat cacccgcatt
     8941 gaaccgagtc tggaagctgc ttttgttgat tacggcgctg aacgtcacgg tttcctccca
     9001 ctaaaagaaa ttgcccgcga atatttccct gctaactaca gtgctcatgg tcgtcccaac
     9061 attaaagatg tgttgcgtga aggtcaggaa gtcattgttc agatcgataa agaagagcgc
     9121 ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg cgggtagcta tctggttctg
     9181 atgccgaaca acccgcgcgc gggtggcatt tctcgtcgta tcgaaggcga cgaccgtacc
     9241 gaattaaaag aagcactggc aagccttgaa ctgccggaag gcatggggct tatcgtgcgc
     9301 accgctggcg tcggcaaatc tgctgaggcg ctgcaatggg atttaagctt ccgtctgaaa
     9361 cactgggaag ccatcaaaaa agccgctgaa agccgcccag ctccgttcct gattcatcag
     9421 gagagcaacg taatcgttcg cgcattccgc gattacttac gtcaggacat cggcgaaatc
     9481 cttatcgata acccgaaagt gctcgaactg gcacgccagc atatcgctgc attaggtcgc
     9541 ccggatttca gcagcaaaat caaactgtac accggcgaga tcccgctgtt cagccactac
     9601 cagatcgagt cacagattga gtccgccttc cagcgtgaag ttcgtctgcc atccggtggt
     9661 tccattgtta tcgacagcac cgaagcgtta accgccatcg acatcaactc cgcacgcgcg
     9721 acccgcggcg gcgatatcga agaaaccgcg tttaacacca acctcgaagc tgccgatgag
     9781 attgctcgtc agctgcgcct gcgtgacctc ggcggcctga ttgttatcga cttcatcgac
     9841 atgacgccag tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag
     9901 gaccgggcgc gtattcaaat cagccatatt tctcgctttg gcctgctgga aatgtcccgt
     9961 cagcgtctga gcccatcact gggtgaatcc agccatcacg tctgcccgcg ctgctccggt
    10021 accggtaccg tgcgtgacaa cgaatcgttg tcgctctcta ttctgcgtct gatcgaagaa
    10081 gaagcgctga aagagaacac tcaggaagtt cacgccattg ttcctgtgcc aatcgcgtct
    10141 tacctgctga atgaaaaacg ttctgcagtg aatgcgattg aaactcgtca ggacggcgtg
    10201 cgctgtgtga ttgtgccaaa cgatcagatg gaaaccccgc actaccatgt gctgcgcgtg
    10261 cgtaaagggg aagaaactcc aaccttaagc tacatgctgc cgaagctgca tgaagaagcg
    10321 atggcgctgc cgtctgaaga agagttcgct gaacgtaagc gtcctgaaca acctgcgctg
    10381 gcaacctttg ccatgccgga tgtgccgcca gctccaaccc cagctgaacc tgccgcccct
    10441 gttgtagccc cagcacctaa agctgcaacg gcaacaccag catctcctgc acaaccaggg
    10501 ttgttgagcc gcttcttcgg cgcactgaaa gcgctattca gcggtggtga agaaaccaaa
    10561 ccggccgagc aaccagcacc gaaagcagaa gcgaaaccgg aacgtcaaca ggatcgtcgc
    10621 aagcctcgtc agaacaaccg ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt
    10681 actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcag
    10741 actgccgaga cgcgtgagag ccgtcagcag gctgaggtaa cggaaaaagc acgtaccacc
    10801 gacgagcagc aagcgccgcg tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag
    10861 gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga aaccgaacag
    10921 gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg
    10981 cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga agaaactgtc
    11041 gctgccgaac caattgttca ggaagcgcca gctccacgca cagaactggt gaaagtcccg
    11101 ctgccagtcg tagcgcaagc tgcaccagaa cagcaagaag agaacaatgc cgataaccgt
    11161 gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt
    11221 cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc aatcgccaat gccgttgacc
    11281 gtagcgtgcg catctccgga actggcctct ggcaaagtct ggatccgcta tccaattgta
    11341 cgtccgcaag atgtacaggt tgaagagcag cgcgaacagg aagaagtaca agtgcagccg
    11401 atggtgactg aggtccctgt cgccgccgct gtcgaacctg ttgttagcgc accggttgtt
    11461 gaagaagtgg ctgaagttgt agaagccccc gttcaggttg ccgaaccgca accggaagtg
    11521 gttgaaacga cgcatcctga agtgattgcc gccgcggtaa ctgaacagcc gcaggtgatt
    11581 accgagtctg atgttgccat agcccaggaa gttgcagaac acgcagaacc ggtggttgaa
    11641 ccgcaggaag agacggctga tattgaagaa gttgccgaaa ctgctgaggt tgtggttgct
    11701 gaacctgaag ttgttgctca acctgccgcg ccagtcgtcg ccgaagtcgc agcagaagtt
    11761 gaaacggtag ccgcggttaa acctgaggtc accgttgagc ataaccacgc taccgcgcca
    11821 atgacgcgcg ctccggcacc ggaatatgtt ccggaggcac cgcgtcacag tgactggcag
    11881 cgccctactt ttgccttcga aggtaaaggt gctgcaggtg gtcatacggc aacgcatcat
    11941 gcctctgccg ctcctgcgcg tccgcaacct gttgagtaat aattagctca agtaatcaag
    12001 ccctggtaac tgccagggct tttttatttc agggtagcat ctttgttgta atgacattag
    12061 cgttacatgc gctgtataat tatcatccat gcaaagccga aggatatatt tatgccagca
    12121 gccaataaca ttccaaccag gcgcgaatct ttaaatttgc gcataaaacc cgcagaacgc
    12181 gatcttattg accgggccgc taaagcccga ggaaaaaatc gcaccgaatt tgtccttgag
    12241 gccgccagag ccgcagctga agaggcgctc attgagcaga gaattttcag gatcgattct
    12301 gatgattacc agctattttt gaatcgtctg gatcagcccc ccgcgcccaa tgctgcatta
    12361 cgcaaaacca tgcaaacacc cgcgccgtgg gagcaaaaaa aatgattcag gcccccgagc
    12421 cactcatggc aaggcatcaa ttcacctctt tttgctcggg agtagaaacg atggataact
    12481 ggctaaagca gagagcccta aaaaatcagc ttgccggcgc atcacgtaca tttgttagct
    12541 gtgatacgta tagcaacgtt ttggcatatt attcgcttgc ttccagcgcc gttgaaacgt
    12601 atgtcgcgac aggacgattt cgccgaaata tgccagaacc gatccctgtt gtagtacttg
    12661 gtcgtctggc aatagataaa tcattacagg gtcagggcat cggtcgtgcg atggtcagag
    12721 atgccggtct acgagtacta caggcggctg aagtcattgg tattcgcgga atgttggtcc
    12781 atgcgttatc agaccaggcc cgtgagtttt atctgcgagt ggggtttgaa ccatcgcctg
    12841 tcgattctat gatactgatg gcaacattgg ctgatttgca ggaatgtctt aaatagttcg
    12901 ttatgtctca tgcaaaaaaa tgcccgcccc ggcgatccca gagcaggcag agaaaacata
    12961 cccagtttca tgatatgttt taaagcgagc ttatttgttg agctggaaca gcgacaatcc
    13021 ctgcatatcg gtaaatgctt tatacgatgc ctgcaatgcc gtttgctgca tgatgtaaga
    13081 tgaaatagtt gcattccagt ccacatcaac cagatcgctc atctgctgcg tttgccctaa
    13141 agcgcgatcg ctacctaatg aatccagcga ctccagttcg ttcagctgcg tgcctaattc
    13201 cgcgcgaaca gtcagcacat tgttcagcga gtttttcagt ccgcggttgg ttttatccag
    13261 tgccgcagcg gcggtttctt tatccgcttc gctatccgcg accggcgttt ttagggctgc
    13321 gatggcgcta tccagcatgg caaaaagatt ggtttcagaa gcgctaccgt ctggttccgc
    13381 taccgcgttg ctggtaatac tgtcgaaaat tttgtcaccc gtgtgcccta tcaccatcga
    13441 acgcgaagca tcgacctgtt gtttaatact ttctgctccg ccttcgtatt tgccatccgc
    13501 ctcactaaac ggcgcagttt ctgttttata accggcaaaa atgtagcgcc cgttaccgtc
    13561 agtggtgttc gccagattca gcaactggtc acgaagcccc tgaatatccg tagccagcga
    13621 ggcacggtca tcgtcactca aggtgccatt gctggcgtag acaatttttt cctgagcatt
    13681 ctggatagcg gtggtgacct ggctaagtac actctcttcc agtgacactt tttgagtggc
    13741 gaaagtacgc gccagcgtgt actggctgtt ttgcgcctgt gcctgggaga gaacaacagc
    13801 ttgtgatgca gcaatgggat catctgaagg gttaacgact cgcttacccg tcgacatctg
    13861 ttcgccgtac ttcatccatt ctgcctgaga attggtgatg ccgcgcatgt tttgctggta
    13921 catcatctgg gtactgaaac gcattttatc tccccttagc gaatgttaat cagcgcatca
    13981 aaaatcgcgt ttgccgtctg cagaacctgc gcattcgcga ggtaatactg ctgaaaacgt
    14041 tgcagatttc cgtactcctc atcgagattg acaccggaaa tcgactgctg ctgattggaa
    14101 agctgcgtca ccacattacc ttgagtggtg ctactggttt tcaacgtcgc ggttttatta
    14161 ccgatatcac tcactaacga ggcataagcg tcgttaaagg atttcgcacc gcccaccgtt
    14221 ttactgttgc tttgcagatc gagcagggcc tgaccgttgc ggttgtcgct atcacccgca
    14281 tcttctgcgc tcgccatggc gattttcgct tcgtcggtga ttaatacatc catgttgacg
    14341 atggcgtcac ttaccggttt cagcgtgaag ctgtcgttaa cggcaggcgt tcctgtaaac
    14401 gtcaactcca gaccatcaaa tgccacttta ccgttggcat ccggcgtcac cgtaaaagtg
    14461 gtattgctgg caaggcgggt gacctgccac tgattattat cgaacgagat tttgtaatct
    14521 gtcgccagta ccgctgaggc atcagttacc gtggcaccga tcgcaacgtc acctttgttt
    14581 ttcgtgtttt gcagaaccgc gggcttaccg atagcaaaga aatcttcacc ggcatcgccg
    14641 ttagcatcaa atccggcttt gtgttgggtg ttgaaagcct cggcaaatgc cagcgccagt
    14701 tgtccaagcg tattacgcgt ctggtcaaga tcctgagaac ggaatgtcag aatgccgccc
    14761 agcgacccgg tattcagtaa tttctccggg atctcaatat tgcctgtcgt cccatcaaca
    14821 taagcgacag tcgtacgaga agggtcggcg ctggaaggaa ctgccgcaag ttgccgcgcc
    14881 gtacttccct gaaccagtga gtagccattg gccatcgtga tgttataagt gccaccatcc
    14941 tgaacgctga cttctacgcc aacaatctgg tttaattcgc tcaccagttg atcgcgttga
    15001 tccagcagat tgttaggtga tgcccctgcc cccacgcctg tcaggcggga gatttgatcg
    15061 ttcaggctgg cgatttgttt agcgtaattg ttgatctgat caacactggc acctatcgcg
    15121 atattgacct gtttgtcctg gtcgcgcaga tattgatcgg tggttttaaa ctgattcacc
    15181 aacccttctg atttcccaat cagcgcctgg cgcgctgccg gatcttccgc gttactcacc
    15241 agcgtttgca ggctggtgaa gaaatcctgc atctgtgttg ccagcgaaga ggtactggtg
    15301 gagagcatat tgtcgatttt cgacatctgc tcatagcggg cagtcaggcc gctactttgc
    15361 gtctgcgccg cacgtaactg gttggtaata aacgcatcat actcacgctg cacaccagaa
    15421 acgtagacgc cattgccaac ccagccgcca gcgcccaacg tgctattggc ctgcgccata
    15481 atagtggttt ggcgggtata tccggcaacg ttatagctgg agatattatt acttgccgta
    15541 tttaacgccg cctgggccgc gttcagtccg ctcatggcgt tattgatcaa gctggacatg
    15601 gaggttcctt ataagccttc aattacagag tattatcggc agcgacccgc cggacttgag
    15661 ttattcagaa cagattatca atgttcatgc tgtaggtttt gctcaccttg tcgcttatcg
    15721 atttcatctg ctgaatcatg ttggtgagtt tgcgggcata gtgaggatcg gtggcatagc
    15781 ccgcgtcctg tagggcctgc gccccctgct ccgcactcgc ggcggtcgtc acggcggcgt
    15841 agcgcgggtt acgcgttaac agcccaacgt aatccgacaa tgcttccaga tacgagctgt
    15901 agacccgaaa cttcgctttt actttcttcg cttcgccatt ttcatattca gtcgtggtga
    15961 tttcagtgac tggccctttc cagttgccag aggctttgac gccaaacagg ttatagctcg
    16021 gctcgccgtt ttcacggcgg atttgccgtt gtccccagcc agattccagc gccgcctgag
    16081 cgaggatcaa atgatgtggc acaccacttt gctggcttgc cagttgggct ggcagcgaga
    16141 gttgcgcgag gaatgcttta ctgtcacccg gcagcgaatc atcgtagtta cgtggtacgg
    16201 ccttttgcac cagctgcgaa agcgtctgat tttgataacg caccacggtt tcgagcggga
    16261 atttcatcgg tgctgctggc gtggactcct ctggcaatgg ttgttctggc gtcatctgtt
    16321 taaccatcat ctctgccagc cccagacctt tgcccgccgt catctgttgg gcaatctgct
    16381 ggtcatacat actggtatac aggcgagtgt gctcgctgct gaacaggcca tcttttggta
    16441 aagcgtcgcg catgcttttc aacatcatct gcacgaacat cccttccacc tggagggcca
    16501 ccggacggat atttgccgcc ggatcttcgc ccgttttcgc cttaagttcg ttgagcgatt
    16561 gtgcatccca ggccgcactt gccagtagtt tgctgtcgct gatcatcaga tgatttccag
    16621 ttttgcccgc agacatcccg cactttgcat tgattgcagt atggacatca gatccatcgg
    16681 cgtagcgccc agcgcattga gcgcacgcac cacgttattg aggctggcgc tggaacgtac
    16741 gctttgcagc gaaccgccgc tctggcgtaa atcgatctgc gtttgtggag taaccacagt
    16801 ctgcccacca ccaaacggtg tatctggctg gctgacattg gcctgacgat taactgttac
    16861 tgagagattc ccctgcgcta ccgcgcagct gtcgagggtc acttcgcgat tcatcaccac
    16921 cgaaccagtg cgcgagttaa tcactacttt agcgtcctgc ggggtgacat taacctgcat
    16981 attctggata tcggcaagga aacggacctg ggaactgttg ccactcggta cgcgcacctg
    17041 aatagtccgc gcatctaacg cggtggcgct gccatatcca cgcacacggt tgatggtgtc
    17101 agcgatttgc tgcgccatac tgaaatcttc gtcgttaagt tgcaaattaa gggtattccc
    17161 gacgccaaac tggctgggca attcacgttc aataaccgca ccattggtga tccgtccacc
    17221 gttcagttgg ttcacctgaa cactgctacc gccagcggag gcccctgcgc cgccaaccag
    17281 aatattgccc tgcgccagcg catacacctg actgtcaacg cccttaagcg gtgtcatcaa
    17341 taacgtaccg ccacgcagac ttttggcatt tcccatggaa gaaaccacca cgtcaatggt
    17401 ttgcccctga cgtccaaacg gtggaagtga cgctgtcacc ataaccgcag cgacgttttt
    17461 tagctgcata ttagtgcccg tcggaacggt aattcccagc tgtgagagca tgttattaag
    17521 cgtttgtgtg gtaaacggcg tctgagttgt ctggtcacct gtaccatcaa gccccaccac
    17581 caggccatag ccaatcagtg agttttgcct taccccctga acactggtga gatcgcgaat
    17641 acgctcagcc tgagccgccg tggtaaccag taaaagaatt aatgcagaga gaaatttaat
    17701 caccacagcc tcacttacat tggtgacagg ttgaggaaga aacgctgcaa ccagcccata
    17761 ttttgcgctt cgttaatgta gccattgcct acgtattcaa tgcgcgcatc cgccacctga
    17821 gtagacggta cggtattgct gccgctgatg gtgcgaggat taactacacc agagaagcga
    17881 ataaattcgg taccctgatt aatggcgatc tgtttttcac ccaccacatg caggttgccg
    17941 ttgaccagta cctggtcaac cgtcaccgtc aacgtgccgc taaaggtatt gctggcattg
    18001 gccccgccct ttccgttgaa cgtgttacca ccggaggctt cgacatcggc acgagcgtta
    18061 ccaaacagcc cctgcaaata gcgcggcaca gtatcaaagc caaaattagt tttaccgtca
    18121 cggctggcat tcgcagagga gcttttgctg gcgctgacgt tctcctgcaa cacgatggtc
    18181 agcgtatcgc caatattgcg tggtcgacga tcttcaaaca acggttgata gccatagtta
    18241 atcggctgag cagactggaa aatagaaccg ttggcgacgg gcgtcggacc gggaaccggt
    18301 tgtgcactgg tcgccccctg taccagcggc gtggagggta tccaggcgca gccggttagt
    18361 gaaagcacca acaagctgga aatggcataa gtatgcgcag cgtttttttg catggctatc
    18421 atcttcaaaa atcagtaaac cggtgaatct gccaccggtt atgccttaga gttgcgtcag
    18481 tttttgcagc atctgatcgg tggtggatac cgctttactg ttgatttcgt aagcgcgttg
    18541 cacctgaatc atattgacca gttcttccgc cacgttgacg ttagacgttt caacataccc
    18601 ttgatacagc agtcccgcac cgttcagacc cggcgtgctt tcgttcggtg caccggagga
    18661 ttgcgtttcg gtgtagaggt tttcgccaat actctccagt ccggtatcat tcataaaggt
    18721 ggtgagattg agctgcccaa cctgaaccgg agctgcctgg ccttgttgtg ttacgctgac
    18781 cacgccatca cgaccgatgg tgatacttag cgcattcgcc ggtatggtga tcgctggctg
    18841 cacctgaaaa ccaccagccg tcaccagctg cccgttctga tccacctgga aagagccgtc
    18901 acgggtatag gctgatgagc catctggcaa catcacctgg aaaaagccct gccctttaat
    18961 cgcgacatct ttactgttat tggtttgcga caggttgccc tgactgtgta agcgctcagt
    19021 agcaaccgga cgcacccccg tgccgatttg taacccggag ggtaaggtgg tttgttcgga
    19081 agactgtgcc cccggctggc gaatggtttg atacagcaga tcttcaaaca ccgcgcgctg
    19141 acgcttaaaa ccgttggtac tgacgtttgc caggttgttg gcaatgacgt ccatattggt
    19201 ttgctgggca tccaggcccg ttttggcgat ccataatgaa ctgatcataa aatgtcctgt
    19261 agtggattag tgacaagcag agcagaagca cgcgacggtg cgcatttccc ctcaccctaa
    19321 cctctcccaa aatgggcgag agaatagtcc gcgcgctttt attgcaatgt catcaatcgt
    19381 ttaattaact catcgacagc agttggttgg cacggcctgc gttatcatcg acgctgctga
    19441 tcaccttcat ctgcatttca aaacgccgcg cgctggcaat catgtcgctc attgccgcaa
    19501 cggcattgac gttactgccc tccagaaccc ccgacatcac acgcaaggtt ggatctgcct
    19561 gcagtaccgg cccacgcgtg gtctggcttt ctgcgcttaa acgaaaaatg ccgtcgtcac
    19621 cgcgctggac ttcgctgccc gtggctttca ccagtttcag acgccccact ggcgcaaccg
    19681 tatttgccgg atcgcccgga ttcagcgccg agattgtgcc gtcggcagca atagtgattt
    19741 ccgccccttc cggcacagca attggcccag cctcgcctat caccggatgt ccctgaattg
    19801 tcagttgccc ggtgggatca acctgaatgc tgccattacg cgtataccct tcgctaccgt
    19861 cagcggtctg cacggccagc cagccatcct gctgcaacgc aacgtccagc gggcgcgagg
    19921 tgtaatccat cttgccgggc gtcatgtctg cacccggcgt tgacgccgtt accagcgtgc
    19981 gcgtgggcag tgaaagccct tccaccggca ctgcgcgcaa ggcgtttaac tgcgcgcgaa
    20041 aaccgggcgt tgaggcattg gccagattac tggcagttac cgcctgttga ttcagcgtct
    20101 ggctggctgc tcccatcgcg gtataaattg cgtgatccat tgagctatcc cgtcagcgat
    20161 tagcgtaagt taaccagcgt gttgaggatc tggtcctggg ttttgatggt ctgggcgtta
    20221 gactgatagt tacgctgggc aacgatcata ttgaccagtt ctttactgag atcgacgttg
    20281 gacgcttcca gcgcaccgtt ggtcagggtg ccaaagttgc ccgtcccggc tgtccccaac
    20341 agcgccacgc cagaagattg cgtcgcagac cagacgttgt cgccttcgga tgccagacct
    20401 tcgttgttgg caaagttcgc cagtacaatc tgccccagca gttgggtttg ttcgttggaa
    20461 tagttgccga caaccgtacc gtcatcattg atttgatagc tcaccagatc gcccggtttg
    20521 tagccgttct gggtggttgc cacaatgttg ttagcgccgg tattttgctg catggagttg
    20581 aggaagctca ggctaaacgt ggcgggaact gcgccgttaa ttgcgccagt ggaaatattc
    20641 gctgttggat ctgaggtcag aacgccattg gcattaaaca ccagtgttgt cgctgtcttc
    20701 gcaatgctgt ttggatcact actatcctgg gtgtagacct gccagttatt atccccggtc
    20761 ttcacaaagt agacgctcat gtcatgagca ttaccctgac tatcgaaaac agtcaccgaa
    20821 ccttttttgt tatagctatc cgcattgctg gcatcaaatg cgttaacaga gggaagcgga
    20881 tcactggaat tcaggttgat ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg
    20941 gtattcggaa tcgaaatatt ggtcggattc gccccttgct gaatagtcgg cggcgtaccg
    21001 gttgccgggt aacccgtcaa ctgtaaacct tgcatattca ccaggttacg gttttcatcc
    21061 agcttaaatt gtccgttacg gctgtagaac accgaaccgt tgctgtccac cagacggaaa
    21121 aaaccgttct ggctgatagc aacgtccaga ccacgcccgg tgttggtggt cgtgccatcg
    21181 gtaaagtcct gggtgatacc ggcaactttt acccccagtc ccactttcga accggcaaac
    21241 atatcggcaa aagaggccgt gcctgattta aagccgtagg tggcggagtt ggcgatattg
    21301 ttgccaataa catcgaggtt ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag
    21361 gccatgactg actcctgaaa tgtgaaggct taaattatct gccgtacttc gtcgagggtg
    21421 gtggtgccgt aagtgccgag atccagcgta ttaccgttgt tgccgcggat cacaccctgc
    21481 accagagcaa actgcagcgg ctgggcaacc agttgtgtac caccgttact ggcgctaatc
    21541 gctacattgt aagaaccgtt cggcgcagtg ctgccgtcag tcaacgtacc gtcccaggtg
    21601 aaactgtgga ctccggcagt cagttcaccg atatcaatgg tacggacaac cgcaccgttt
    21661 ttatcggtga tggtggccgt aactttgtct gccgcctgtt gcagctcaac accaaacggc
    21721 gtggtcgtgg tcacagcccc ttcttcacta ccggttcccg caagaacagt ggtgccgggg
    21781 atcatcacgc cgtgaccgat caggttactg gcctgtaacg actggctgtt atcaatctgt
    21841 ccggaaatag atccgagcgt ggtattgagt ttttcaatcc cgctgaccgt gctgatttga
    21901 gccaattgcg acgtcagctc gttgttttcc attggattgg tcgggtcctg gtttttcagc
    21961 tgcgccacca acaaagtcag aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc
    22021 gaactactac tggtggtgct gacgccggta tttgtcggat cggtggtggt taccgcaatg
    22081 gacatagctt tctcctttat tgaccgagcg taagggtttt cagcatcatg cttttcaccg
    22141 tgttgagcac ttcaacattg gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct
    22201 ccccgacgac atcaacgttt ggcattttca cgtagccctt tgcatccgcc agcggattac
    22261 ccggttcata aaccagtttg tccggggcct gactttctat aacatcggca acctttacgc
    22321 cgcctgtcgc agcacctggt gcagcgttaa cctgaaatac cacctgtttt gcccgatatg
    22381 gctgtccatc gggaccggtc acgctatcag cattcgccag attactggcc gccacgttca
    22441 ggcgctggga ctgggcagtt aacgccgacc cggcgatatc aaaaatattc agcagtgcca
    22501 tccgttaatt tccgctctgt aaaacgttca tcatgccttt gatttgcccg ctcaacgcgc
    22561 taaggctcat ctggtattgc aggctgttat cggcaaactg ggtacgttcg cgatccatat
    22621 cgacggtatt accgtcaagc gaaggctggt ccggaatacg gtattgcagt tctgcggtag
    22681 gaggcgtcag cgcctgcgcc ggaatgtgtt gcgttgaggt catcgtcagt gcaaccacgc
    22741 ttgttgcatc ccgtccacgt tgcatgactt ttttaagttc actggcaaaa tcgatatcgc
    22801 gcgcctgata accaggggta tcggcattgg cgatgtttgc tgccagcact tcctgacgct
    22861 gggcgcgcag attgagcgcc tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca
    22921 tatctcctcc gcaggtatca aaattctgcc atcagcttaa atgcctttac gcgcgcgtta
    22981 tcggcggaat aaacgcaaaa tgggtcgcta tttatgccgt tgatggtcat tgcggacagg
    23041 tacaattcac gttgtagaaa tggcaggggg cgaaaatgct ggcaataaaa cgtagcgtgg
    23101 caatcatcgc gatactgttc agtccgttaa gtgcggcgag caatctcaca tcgcaattgc
    23161 acaccttttt tagcgcccag ctcgcggggg taagtgatga ggttcgtgtt tctattcgta
    23221 cagcgcccaa tctactaccg ccgtgcgagc agccattgct ttcgatgagc aataattccc
    23281 gcctgtgggg caatgtgaat gtgttggcac gctgcggtaa cgacaaacga tatttacagg
    23341 ttaatgtgca ggccacagga aattatgtgg ttgccgcaat gcccattgtg cggggaggaa
    23401 agctggaagc tggcaatgtc aaactgaaac gcggacggct ggacaccttg ccaccgcgta
    23461 cggtgctgga tatcaatcaa cttgttgatg ccgttagcct gcgcgatcta tcacccgatc
    23521 aacctatcca gttaacccag tttcgccagg catggcgggt aaaagcggga caacgcgtca
    23581 acgtgatcgc cagcggtgat gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg
    23641 ctgccgtcgc acagaatgcg cgggtgcgca tggtatcggg acaggtggtc agcggcgttg
    23701 ttgatgcaga tgggaatatt cttataaacc tgtaagctgt taaagattac ccgtcccttg
    23761 ccgataaata agcaacacat gataaaagcg ccctcaatga ggaataaaca atgagtattg
    23821 atcgcacttc gcctctgaag cccgtaagca ccgttcaacc gcgcgaaacc actgacgcgc
    23881 cggtaacgaa cacccgggcg gcaaaaacaa ccgcctccac cagcaccagt gtgacgttaa
    23941 gcgacgcgca agcaaaactg atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg
    24001 aagcgttaaa actggcgatt cgtaacggtg aactaaaaat ggacaccggc aaaattgccg
    24061 atgcgctgat caacgaagcg cagcaagact tgcaaagtaa ctgatcgtat gacgcgtctt
    24121 gcagagatcc tcgaccagat gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa
    24181 gagcagcaac atctctctat ggggcagatc aacggcagtc agttgcaatg gattacagaa
    24241 caaaaaagct ctctgctggc gacgctggat tacctcgaac agttacgcag gaaagaaccc
    24301 aatgcagcaa atagcgttga tattagtcaa cgctggcagg aaattactgt gaaaacgcag
    24361 caactacgcc aaatgaatca acataacggc tggttactgg aaggacagat tgagcgcaat
    24421 caacaggcgc tggaaatgtt gaaaccgcat caggaaccga cgctatatgg agcgaacggt
    24481 cagacctcaa caacccatcg cggcggtaaa aagatttcga tctgaagatt atctccggcc
    24541 tgcactgcag gccggaatgc attgttacac cgtccggcgg gcaaactctt taactttgaa
    24601 gcccagtacc gccagtgcag caaagtacgc agcaatcccc gccagcacga ccgccattaa
    24661 acgcagtaag cgccagggca tggtacccaa tgaccactcc ggcatgatat gtaacatacc
    24721 taaaagcacg cccgacatca ccagtaccgc caccaccaga cgcaacagaa acgccatcca
    24781 gccaggttgc ggggtaaaga ttttctgctt acgcaactgc cagtaaagca gcgaagcatt
    24841 cagacacgcc gccagaccaa tagaaagtga cagcccggca tgtttcaacg gaccgataaa
    24901 cgcaaggttc atcaattgcg ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt
    24961 aatgtcctgg cgggaataaa agccaggagc caacactttc actacaatca ggccaatcaa
    25021 acccaccgag taggcaatta acgcccgctg ggtcatcagc gcatcaaagg cggtaaattt
    25081 accgtactgg aacagcgaaa cggttaacgg acctgaaaga atgcccaacg caaccgcact
    25141 cggcagcgcc aacaggaaac aaagacgcag cccccagtcc atcaaacggt tgtattcatc
    25201 atgattgcca ctggcaaaac ttttcgacag cgacggcagc aaaatggtgc caagcgccac
    25261 gcccagtaca ccggacggaa actccattaa gcggtcggcg taatacatcc aagacaccga
    25321 accggaagca agaaacgagg caaaaatggt gttgatgatt aaggagatct gactcacaga
    25381 gacgccaagg atcgccggtc ccatctgttt caccacgcgc attgctccgg catcgtggaa
    25441 gttaatgcgc ggcaggacca gcatgccgat cttcttcagg tgcggtagct gatacaccag
    25501 ctgcaggacg ccgccgaccg taacagccca cgccagcgcc agcaccggtg ggttaaagta
    25561 cggtgcggca aacagcgcga aaccaatcat gctgatattg agcaatgtcg gggcaaacgc
    25621 cggaatcgag aagcggttcc acgtattcag aatcgctccc accagcgacg ccagggagat
    25681 cagcaagata tagggaaagg taatctttag tagctggctg gtcagggcaa atttgtcagc
    25741 tgtgtcagcg aagcctggcg cggtcaccat gatcacccac ggtgcggcga gcatgccagc
    25801 gaccgtcaca accgccagcg caagtgtcag caggccagaa acataagaga caaagacccg
    25861 cgtggcgtct tcaccctgct tacttttata ttccgccaga atcggtacaa atgcctggga
    25921 aaatgcccct tcggcaaaga tacggcgtaa caagttagga agtttaaaag cgacgaaaaa
    25981 ggcgtcggtt gccatccctg cgccaaagat tctggcgaca attgcgtctc gtgcgaagcc
    26041 aagcacacgc gaaaacatgg tcatcgagct gacggcggcc agcgatttta ataaattcat
    26101 cggtgttcta atccagaccc acgggcaaaa cgcctgcaag gcaggcgctg aaagaggcga
    26161 atagtctacc tggattatgg tgaattgcta ccgccagatg ttacagggtt tattcactca
    26221 tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag cactgcctgt tcgccggcgg
    26281 tttgcggaac tgtctgattt tgcacgcatt caatgaagtg ccgcgcacag ccgacaaatc
    26341 cacgttgctc aagcgtactc tgccaaccag gaatcggttt attcactaca ccctgcccac
    26401 gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag cgcaccgtca gtcacggcct
    26461 gcacggtttc acgctggctt ccggcacggc gatgcataca ggtggtgatc tgcaaaggac
    26521 cggccgaaaa atggtgctcg gcaaacagca tttcgccagc gtcattagtc agtagcgtac
    26581 cgccatccag agaggcttta ccgcccgaca accacagcgc ggtatccacc acatgcagat
    26641 aatcatccag caacgtgaaa taaagatcgt gtggcccgac gctattgcta cgatgtttat
    26701 ccattcttag cgaggctgcg gtggcgagtt gcgtttttaa ctcaccgtag agtggtgcga
    26761 aacgacggtt aaaaccgacc atcaacgtca gttttttccg cgccgcaagt tccaccagcc
    26821 gttcagcatc gcgcagattt tctgccagcg gtttatcgac acagacatgt acccccgcat
    26881 tgagtaacgt actgaccacg tcaaagtggc tggcggtgct ggaatgcaca aaaaccgcat
    26941 cgcaactggc ggcaaggctg gataacgaat cggcataagg aatgcgccag ctttcacaaa
    27001 ttggcagggc tttcgcgcgc gtaggcgacc aggctccttg taacatccag tcagacgctg
    27061 ccgccagcac cggtaaccac gccttttgcg cgatgccgcc taaccccact acgccgatac
    27121 gtaatttttt cactttaatc tcccagatgg gccagcaacg aatcaagacg ctgtttcagt
    27181 tctgccactt cgatttccag agcttcgacg cgggcctgta aatcaccgtc aaccgcgttc
    27241 gacgtatccg ttaccgccgg ctgatcttca acctcaccac tgaaaagatg catgtagcgg
    27301 ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa aaggaccatc ttcgcgattc
    27361 gccagttgtt ccagcgtcga ctccacttcc gccatatcgc tgaattcata cattcgcgct
    27421 gcgcggctgc gcagttcacc cggcgtctgg gcaccacgca ataacaacgt ggtgattaac
    27481 gccacttctg cggcgctcag tttcagatcg ccaaattctg aattacaaaa acgttgctca
    27541 tatttggtga cccgattacc aaaaccgctc actgtgcgta gataatgacg tttgaccaga
    27601 ttatccagct gttcctgcac ttcggattca ctcagattca tgaccggttc acggttcgtt
    27661 ttctgattac aggccgtgac tacaccattg actgagagcg ggtattgctc cggcgtggtc
    27721 acctgctttt ccagcaagca accaatcacg cgagcttcaa gggcagtaag ttgatatttc
    27781 atgagattct ccttagcggc cgggcgtcca gtctggggta gttaatgccg tcagtacgtg
    27841 atcgcgccat tgtccatcaa tcaacagata gtctttcgca tagccttctt tttcaaaacc
    27901 cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc atataattag ccataatgcg
    27961 atgaatatgt tgggtgcgct gcatataacg aatggctgcg gtcagggctt caaacatgag
    28021 tcctttgccc tgccattttt gcccaatcga ataaccgaga tagcaggcat gaaaagagcc
    28081 acgaacaaca ttggaaaaat tggcaacgcc aataatctct ttttcgtccg ggtcgaataa
    28141 gccaaagtag aaagctgaac cttgtttatg aaattcgtta atcatcccta gcctggcctg
    28201 ccagcctgat ggataacagt ggctttcgtc gcgcactggc tcccagggct tgaggaaatg
    28261 gcgattctct gcgtaataat ccgccagacg ccaggcatca cgatcatgca ccagacgcac
    28321 gaccagtcgg tctgtggtta agcgcacttt tggcacgtta ctgcgatagc caaacattct
    28381 atacctactc cttcccgtaa cgtctttaat acgctgtaag tattactata cctgcgcaac
    28441 cagaccctgt gaaaagtcca gaatgccatt ttctgcatta ttcacatatt tgataaaaac
    28501 tctctatcaa agcgacgttt tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct
    28561 tagcggcaat gcagaataga aagtgctcat cttttatttc ccgggagggg aaatgtcccg
    28621 cgtgtcgcag gccaggaacc tgggtaaata tttcctgctc atcgataata tgctggtcgt
    28681 gctggggttc tttgttgtct tcccgctgat ctctatccgc ttcgttgatc aaatgggctg
    28741 ggccgccgta atggtcggta ttgccctcgg cctacgccag tttattcagc aaggtctggg
    28801 tattttcggc ggagcaattg ccgaccgctt tggtgccaaa ccgatgattg ttaccggtat
    28861 gctgatgcgt gccgccggat tcgccacaat gggtatcgcc cacgaaccgt ggctactgtg
    28921 gttttcatgc ctgctttcgg gactcggcgg cacgttgttt gatccgccgc gttcggcgct
    28981 ggtggtgaaa ttaatccgtc cacagcagcg tggtcgtttt ttctcgctgt tgatgatgca
    29041 ggacagtgcc ggtgcggtca ttggcgcatt gttggggagc tggctgttgc aatacgactt
    29101 tcgcctggtc tgcgccacag gggcagtttt gtttgtgctg tgtgcagcgt tcaatgcgtg
    29161 gttgttaccg gcatggaagc tctccactgt acgcacgccc gttcgcgaag gcatgacccg
    29221 cgtgatgcgc gacaagcgtt ttgtcaccta tgtcctgacg ctggcgggtt actacatgct
    29281 ggctgtacaa gtgatgctga tgctgccaat tatggtcaac gacgtggctg gcgcgccctc
    29341 tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg ttaacgttgc tctatcctat
    29401 cgcccgctgg agtgaaaagc attttcgtct ggaacaccgg ttgatggctg ggctgttgat
    29461 aatgtcatta agcatgatgc cggtgggcat ggtcagcggt ctgcaacaac ttttcaccct
    29521 gatttgtctg ttttatatcg ggtcaatcat tgccgagcct gcgcgtgaaa ccttaagtgc
    29581 ttcgctggca gacgcaagag ctcgcggcag ctatatgggg tttagccgtc tgggtctggc
    29641 gattggcggc gctattggtt atatcggtgg cggctggctg tttgacctgg gcaaatcggc
    29701 gcaccagcca gagcttccat ggatgatgct gggcattatt ggcatcttca ctttccttgc
    29761 gctgggttgg cagtttagcc agaaacgcgc cgcgcgtcgt ttgcttgaac gcgacgcctg
    29821 atttgatact cccccctgac tcctccatac tgaaagtgaa ctgacagcca aatggaggag
    29881 tcacgtgaag ctatacattt acgatcactg cccttactgc ctcaaagccc gcatgatttt
    29941 cggcctgaaa aatatccccg tcgaattaca tgttctgctc aacgacgacg cagaaacacc
    30001 cacccggatg gtcggtcaaa aacaggttcc cattctgcaa aaagatgaca gccgctatat
    30061 gccagaaagc atggacatcg ttcactatgt cgataaactc gacggcaaac cgttactgac
    30121 cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag gtcaatggct acgccaacaa
    30181 actgctgttg ccgcgttttg ccaaatcggc atttgatgag ttttctactc ctgccgcgcg
    30241 caaatatttc gtcgacaaaa aagaggccag cgcgggtaat tttgccgacc tgctggccca
    30301 ctctgacggt ctgattaaga atatcagcga tgatttacgt gcgctggaca aactgatcgt
    30361 caaaccgaac gccgtgaatg gcgaactttc ggaagatgat attcagctat ttccgctcct
    30421 gcgtaatctg acgctagtag ccggaatcaa ctggccaagc cgcgttgctg attaccgcga
    30481 taatatggcg aaacagacac aaatcaattt gttatcatca atggcgattt aatcctgacc
    30541 cgccgcggca gcctgtattc gcgggttgcc gccgttttca gttttttctg ctggcgcgtt
    30601 tatcgctggt gacacatgct gcgttgtgtc aggatattga cgcagatttc atgtcgagga
    30661 ctaccatgaa caagttttta tttgccgctg cattgatcgt cagtggcctg ctcgttggct
    30721 gtaatcaact cacccaatac accatcaccg aacaagaaat taaccagtcg cttgcgaaac
    30781 ataataattt ctcaaaagat atcggtttac ccggcgtggc tgacgcccat attgttctga
    30841 caaacctgac cagccaaatt ggtcgcgaag agccgaataa ggttacccta accggagacg
    30901 ccaatctgga catgaactcc ctgttcggta gccagaaagc gaccatgaaa ctgaagctga
    30961 aagcgctacc cgtgtttgat aaagaaaaag gcgcgatctt cctgaaagag atggaagtgg
    31021 tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg cttccctatt
    31081 tgaaccaggc attacgcaat tactttaacc agcaacctgc ttacgtcctg cgcgaagatg
    31141 gcagccaggg cgaagcaatg gcgaagaaac tggcgaaagg cattgaagtg aagccaggcg
    31201 aaattgtcat tccgtttact gattaatcac gagggcgcat tcgcgccctt tatttttcgt
    31261 gcaaaggaaa acgtttccgc ttatcctttg tgtccggcaa aaacatccct tcagccggag
    31321 catagagatt aatgactgca ccatcccagg tattaaagat ccgccgccca gacgactggc
    31381 accttcacct ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc agcgaaattt
    31441 atggacgggc tatcgtaatg cccaatctgg ctccgcccgt gaccaccgtt gaggctgccg
    31501 tggcgtatcg ccagcgtatt cttgacgccg tacctgccgg gcacgatttc accccgctga
    31561 tgacctgtta tttgacagat tcactagatc ctaatgagct tgagcgtgga tttaacgaag
    31621 gcgtgttcac cgctgccaaa ctttacccgg caaacgcaac cactaactcc agtcacggcg
    31681 tgacgtcagt tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgt
    31741 tactggtgca tggtgaagtg acacatgcag atatcgacat ttttgatcgt gaagcgcgct
    31801 ttattgaaag cgtgatggaa ccgctgcgcc agcgcctgac tgcgctgaaa gtcgtttttg
    31861 agcacatcac caccaaagat gctgccgact atgtccgtaa cggaaatgaa cggctggctg
    31921 ccaccatcac tccgcagcat ctgatgttta accgcaacca tatgctggtt ggtggcgtgc
    31981 gtccacatct gtattgtcta cccatcctca aacgcaatat tcaccaacag gcattgcgtg
    32041 aactggtcgc cagcggtttt aatcgcgtat tcctcggtac ggattctgcg ccgcatgcac
    32101 gtcatcgcaa agagagtagc tgcggctgcg cgggctgctt caatgcccca accgcgctgg
    32161 gcagttacgc taccgtcttt gaagagatga atgctctgca gcactttgaa gcattctgtt
    32221 ctgtaaacgg cccgcagttc tatggcttgc ctgtcaacga cacattcatc gaactggtcc
    32281 gcgaagaaca acaggttgct gcaagcatcg cactgactga tgacacactg gtgccattcc
    32341 tcgccgggga aacggtacgc tggtccgtta aacaataaaa atcgtagctt cctgttgtca
    32401 ttaggttatt ttacctgtat aaataaccag tatattcaac agggggctat tatgcgaatt
    32461 gaagtcacca tagcgaaaac ttctccattg ccagctgggg ctattgacgc cctggctggc
    32521 gaactttccc gccgtattca gtatgcgttt cctgataatg aaggccacgt atcggtacgt
    32581 tatgccgcag cgaataattt atcggttatt ggcgcgacaa aagaagataa acagcgcatt
    32641 agcgaaattc tccaggaaac gtgggaaagc gccgatgact ggtttgtcag cgaataatat
    32701 gcagtgattt ttgtttttgc cgggtcgccc cggctttttt attttactta caaatttcga
    32761 gtacaaaatt tctacacttc ctcaaaataa ttgcacaata tgctaaaaaa atatgaatga
    32821 catgctgttt tattgttcat ataaatctta aaagcaaaaa tttatctgtt ccccccttta
    32881 aaaattcggt gaataccctt acttattggt atactgaagt tgcttcagat aaaaacgcat
    32941 tgaacctcga ataacgttgt ctagtaacac gaattagggg gccatgatgg aaaaaaataa
    33001 tgaagtcatt cagactcatc cgctcgtagg gtgggacatc agcaccgttg atagctatga
    33061 tgcgctgatg ttgcgtttgc actaccagac cccaaataag tccgagcagg aagggactga
    33121 agttggtcag acgctctggt taaccactga tgttgccaga caatttattt cgatattaga
    33181 agcaggaatc gccaaaattg aatccggtga tttcccggta aacgagtatc ggcgtcatta
    33241 acatgacatg accatccacc caaaaggcac ctcaccggtg cctttaccat ttctgggttg
    33301 tatatctgca ttcagttaat tactctgctt aaaccatgag agcagagaga aaaaaatgaa
    33361 atacgatctc atcattattg gcagcggttc cgtaggcgct gccgccgggt attatgcaac
    33421 ccacgccggt ttaaacgtgc ttatgaccga cgcccatatg ccaccacatc aacacggcag
    33481 ccaccacggc gatacgcgat taattcgcca tgcttatggt gaaggcgaaa agtatgtccc
    33541 gctggttctt cgcgcgcaaa cgctgtggga tgaactctcc cgccacaacg aagaagatcc
    33601 catttttgta cgctctggcg tcattaacct tggcccggct gattccacat ttctcgccaa
    33661 cgtcgcccac agcgccgaac aatggcaact caacgttgaa aagctcgatg cgcaagggat
    33721 tatggcccgc tggccggaaa tacgcgtccc ggacaactac atcggcttat ttgagactga
    33781 ttccggtttt ttgcgcagcg aactggcgat taaaacctgg atccaactgg cgaaggaagc
    33841 gggctgtgcg cagctgttca actgcccggt caccgcaatt cgtcatgacg atgatggcgt
    33901 aactattgaa acggctgacg gtgattatca ggcgaaaaaa gcgattgtct gcgcgggaac
    33961 atgggtaaaa gaccagctcc cggagctgcc tgtccagccc gtacgcaaag tatttgcctg
    34021 gtatcaggcc gatggtcgct atagcgtgaa gaataaattc ccggcgttta ccggtgaact
    34081 gcccaatggc gatcaatatt atggttttcc ggcagaaaac gacgcgttga agattggcaa
    34141 acataacgga ggccaggtta tccattcagc ggatgaacgt gttccgtttg cggaagtggt
    34201 cagcgatggt tcggaagcct tcccgttctt gcgcaatgta ttgccgggta tcggttgctg
    34261 cctgtacggc gctgcctgca cctatgataa ttcgcctgac gaagatttta ttatcgatac
    34321 cctacccggc cacgataata cactgctcat taccggcctg agtgggcacg gttttaaatt
    34381 tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa gaaaaaaaaa gcgattttga
    34441 tttgacgcca ttcaggcttt cccgcttcca ataatcatca ttacggcctc cagaatgagg
    34501 ccgtttttac aggagtattt aatgcgtcgg ctgttgcact atctcatcaa taatattcgc
    34561 gagcatctga tgctttatct ttttctctgg ggattgttgg caattatgga tctgatctat
    34621 gtgttttatt tttagttttc cgcactgaca taaactgaca aatatttaat ctccctttca
    34681 tttagcgtga aaaattgaat gagattattc ctgcttctac agcactcacc agacatctct
    34741 gatgctgaac ccactaaact tatgaagatt ctttcattat taattcaaaa ttattgaaat
    34801 aaaaataaat atcattcaat ttgttatttt tgtgttgcaa ttcattctcc gacacgccat
    34861 tttctttctc tcgcaaattc gttttggatg cacttatgtc attcacaaat acccctgaac
    34921 gctatggtgt tatatctgcg gccttccatt ggctaagcgc aattattgtc tacggcatgt
    34981 ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat cacaaagcac
    35041 ccgaactgca taaaagcatc ggtattttgt taatgatggg gctggttatt cgcgttctgt
    35101 ggcgcgtcat atctccaccg ccgggtccgc tgcagagtta ttcgcccatg actcgtcttg
    35161 gcgcaaaggc tggacatctc gcattgtact tattgctgtt cgccatcggc atcagcggct
    35221 atctgatctc aacagccgat ggtaaaccga tcagcgtttt tggctggttt gacgtccccg
    35281 cgaccctcgc cgacgctggc gcacaggctg actttgccgg tgccctgcat ttttggctcg
    35341 cgtggagcgt cgtagtactg tccgttatgc acggatttat ggccctgaag catcatttca
    35401 tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac tatggagtat
    35461 aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcttc cctgatgttc tctgccggtt
    35521 cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt gttaacttcc
    35581 gcatacagca ccttggctat agctggttat acggcacctt taaagatttc gacggtactt
    35641 ttacctttga tgaaaaaaat ccagctgccg ataaagtgaa tgtgacaatt aacaccacca
    35701 gcgtcgatac taaccacgcc gaacgcgata aacatcttcg cagtgcggat tttctcaata
    35761 ccgcaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac ggtgacgacc
    35821 tggatattac cggcgatctg acgctgaatg gcgtaaccaa gcccgtcaca ctggaagcga
    35881 aattaattgg tcagggcgac gacccatggg gtggtaaacg tgcaggcttc gaggccgaag
    35941 gcaaaattaa gctcaaggac ttcaatatca agacagattt aggtccagct tctcaggaag
    36001 tggatctgat tatttcagtg gaaggcgtac agcagaagta agagtgaaat gttgccggat
    36061 ggcacgcatc cggcaatgat atttattccg ttggatcagg aatgcccagt gttgtattca
    36121 gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc
    36181 gctgttcttc aggcggtaac gcgctttctt cacagcaaat ctcactacaa caacctttgt
    36241 atttttccgc acatactgga cactgaataa aaagcaggtg gcagccatca tttttacagt
    36301 tggtatggct gtcgcacggc gcaccgcact ggtggcaatg cgcgataatc tcatcagata
    36361 tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgt accggtaaac
    36421 cctgctcgcg cgccttgcgg gcgtattcaa taattccgcc ctcgatatgc cagactttat
    36481 tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt
    36541 acataacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt ggcagctgct
    36601 cacggaaggt atctgccgga atttccagcg cgttttcaaa gtgccccact tcatactcat
    36661 agtggttacg catgtcgata aacaatgcat cgggatcgtc aagcatagcg ttcacttccg
    36721 ccgcttgcag atactcaccc acattgctgg catcaaagtg aggatcgtca ataccgtcgg
    36781 caacgatacg atcgcggacc ttcatgcgca gtacccagaa ggatttcccg tcatcatcca
    36841 acgcgatatt caggcgtaag ccctctaaag ccgggtcgaa ggcataaagc tgcgcgcgaa
    36901 atgtttcaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat
    36961 acactcgccc aaaaacatcc agcgcggtaa acagctgata taaagcttca cgggtcgcct
    37021 taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt tcgctatcag
    37081 ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt
    37141 acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat
    37201 taacatccac acattttacg ctacatttgc gcattaaaaa ttatttatta tttacaagcg
    37261 cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa atgcgacaat
    37321 acatacaatt gcccgaatag gttgaaaaac aggattgata tgacgaatct acccaagttc
    37381 tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg
    37441 ttagtcgtgc aattgcccta cccggttatc taccgcctcg gttgtggatt aggaaaactg
    37501 gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc
    37561 ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc
    37621 atgggactga tggaaactgg catggcgtgg ttctggccgg accgcagaat cgcccgctgg
    37681 acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg
    37741 ttagttggca tccattttct gacactggag ctgggtgcgc ggcagtttgg tatgcaggaa
    37801 ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg
    37861 ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa
    37921 gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc atgattacgg cccgcgctca
    37981 agcgttttcg tcccgttgtt tgccgttgag caggctgcga ccacgaccgg aacctggatg
    38041 ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc
    38101 aaagggtatc agttgattat gctgccgcca gagtgttctc cgccgctgga tgatgccgaa
    38161 accaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc accagagcag
    38221 tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc acgctattaa
    38281 atctcccatg ccggatgctt cagaagggta tccggcatta ccacagcaaa tccccctgat
    38341 ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa ttagtgtgct
    38401 tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc tatgtcaccc
    38461 tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg gctaggctgt
    38521 tttcttaccg gtgccgcctt cagtctggta atgcccttct tacccctcta cgttgagcag
    38581 cttggcgtta ccggtcactc cgccctgaat atgtggtccg gtattgtctt cagcattaca
    38641 tttttatttt cggccatcgc ctcaccgttt tggggtggac tcgccgaccg taaaggccga
    38701 aaactcatgc tattacgctc tgccctcggc atgggcatcg tgatggtgtt gatggggctg
    38761 gcacaaaata tctggcagtt tttgatcctg cgggcgcttc ttggtttact tggcggattt
    38821 gtccccaacg ctaatgctct tatcgccaca caagtaccac gtaataaaag cggctgggcg
    38881 ctggggacgc tctccacagg cggcgttagc ggtgcgttgc ttggcccaat ggctggcggc
    38941 ctgctcgctg atagctacgg cttacgtccg gtattcttta ttaccgccag tgtgctcata
    39001 ctctgctttt tcgtcaccct gttttgcatc agagaaaaat tccagccggt cagcaaaaaa
    39061 gagatgctgc acatgcggga agtggtgaca tcacttaaaa acccgaaact ggtactcagc
    39121 ctgtttgtca ctacgttaat catccaggtg gcgacgggct caattgcccc cattctgacg
    39181 ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg cctttatcag tggcatgatc
    39241 gcctcggtgc caggcgtggc ggctctgctg agtgcaccac gactcggcaa acttggcgat
    39301 cgaatcggac ccgaaaagat cctgattacg gcgctgatct tttctgtact gctattgatc
    39361 ccaatgtctt acgttcagac gccattacaa cttgggattt tacgtttttt gctcggtgcc
    39421 gccgatggtg cactactccc cgccgtacaa acactgttgg tttacaactc gagcaaccaa
    39481 atcgccgggc gtatcttcag ctataaccaa tcgtttcgtg atattggcaa tgttaccgga
    39541 ccattgatgg gagcagcgat ttcagcgaac tacggtttca gagcggtatt tctcgtcact
    39601 gctggcgtag tgttattcaa cgcagtctat tcatggaaca gtctacgtcg tcgtcgaata
    39661 ccccaggtat cgaactgatt tttcgccttt catacttgca aaagcggaga atcagctatc
    39721 cttttccctg aaacctcatc aactcaaagg gagaatcgtg atgaccatgt acgcaacgct
    39781 tgaagaagcc attgacgctg cacgcgaaga atttcttgca gacaaccccg gcatcgacgc
    39841 cgaaaatgcg aatgtgcaac agttcaatgc ccaaaaatac gttttgcagg acggcgacat
    39901 catgtggcaa gttgagtttt ttgccgacga aggggaagaa ggtgaatgtt tacctatgct
    39961 tagcggtgaa gccgcgcaaa gtgtttttga tggcgactat gatgagatag agatccgcca
    40021 ggagtggcag gaagagaata cattacatga atgggacgag ggggaatttc agcttgagcc
    40081 accgctggat accgaggaag gacgcgcagc agctgatgag tgggatgaac gttaatcact
    40141 catacgggcc atgatgaatg tcgatcggca gcagtaaagt atcaaaaacc agggagaatg
    40201 gcagatcaag gatcgtgaca taacgccagg cggagtcacg cacatcccat tgcacaccgg
    40261 gataatattg gttgccatgc ccctgccccg gtatagtgcg actaataatg ctgccacagc
    40321 cactcagcaa ggtcaccatg atgctcacca caattaaacg catttgatac ctcttccgta
    40381 aaaaaatacc ggcataacgc cggtattttt attggctcgt tgtttttgcc agatgtggcg
    40441 tgaacgcctt atccgaccta caaaatcttg caaaatcaac acattgcagg agcgatgtag
    40501 gcctgataag cgtagcgcat caggcaacta cgtttttatt gcgaagccgc atccggttta
    40561 cgcaatgcca gtggattgag ctttatccct tcgtaataac tcacccatga agaatatctc
    40621 tcaggggaat tccagacgcg gaaatgcaga cgggccatcg tcaccggatc gcttagcagc
    40681 accaggcgac gatcgcgatt cagcttctct ggcgtctcgt tcagcgcctg ttcaacgtgg
    40741 cggtcacggg cgatttccag caccttactg gcccggtgac gcgcggtcgc cattgcggtt
    40801 gccagagcgt taaatgacgg gttaaacact gcgtgcataa agccatcgtc aagggagcgt
    40861 tgacggttca tctcaaggaa ccgatcggta tcaaccagca cctgcggcgg tgaatactct
    40921 tccgggatca ggaacagttt ccagcgtttg gtgcgcagac caacggtggc acggctggaa
    40981 atcaccgaaa caaacggtga caggatcaac gagaagacaa tcggagccag ccagaacagg
    41041 aaacgcagat ccagccacgc catcccaaca gcccacacta accctaacag cagctgtgag
    41101 ccgtggcgtt tgaacgcttc accccaggaa gtggagtcat catcacgctg cggtgaattc
    41161 cacaccactt cccagccaag gaacgcgctg acgacgaaga ccgtatggaa cagcatgcgt
    41221 accggagcca gcagcacgga aaacagcact tccagcagca acgataatgt aacgcgccag
    41281 aagccgccat attctttcgt tcctttgcac caaataagca aaatgctcaa tagctttggc
    41341 aggaacaaca gcaccatggt cgaagcaaaa agtgcaatcg ccagctcagg acgccactgc
    41401 ggccacaccg ggaacaactg ccgtggttgc aggaagtatt gcggttcggt caacgcatgc
    41461 actacctgca atgcagtaga gagcgcgagg aacataaacc acagcggagc ggagagataa
    41521 gacatcacgc ccgtcagaaa taccgcacgg tgaaccgggt gcataccctt caccaggaac
    41581 agacggaagt tcatcaggtt accgtggcac cagcggcggt cacgttttag ctcatcaagc
    41641 aagttaggcg gtaattcttc ataagaaccc gggagatcgt aagcaatcca gactccccaa
    41701 cctgcacggc gcatcaacgc agcttccacg aagtcatgtg acaggattga accggcaaag
    41761 gaaccttcgc ccggcagcgg agccagtgcg cagtgctcga taaacggttt cacgcggata
    41821 atcgcgttat gtccccagta gtgcgactcg ccaagttgcc agaagtgcaa accggctgta
    41881 aacagtggcc catacacgcg agtcgcgaac tgctgacagc gtgcatacag cgtatccata
    41941 ccggacgctt tcggcgacga ctgaatgatc ccggcgttcg ggttggcttc catcaaacgc
    42001 accaggccgc ataaacaatc accggtcatt accgagtcag catccagcac caccatgtag
    42061 ctgtactggc tgccccagcg acggcagaag tcatcgatat taccgctttt acgcttcacg
    42121 cggcgacggc ggcggcgata gaaaatctga ccttcgccac cgacttcagc gataagctcc
    42181 atccaggctt tttgctctgc gacgcagata tccgggttat aactgtcact cagaatgtag
    42241 acatcaaagt gtttggcatt cccggtggct tttactgatt cccacgttgc acgcaagcca
    42301 gcaaaaacac ggttcacgtc ttcgttacag ataggcatga tcaacgccgt gcgatgctcc
    42361 gggtttaatg gttcatcgcc aactgttgac gcagatatac tgtatttatc gcgaccaata
    42421 agcagttgca ggaagcccat caacgccgtc cagaatccgg cggacaccca acagaacagc
    42481 accgcaaaga ggatcaggat accggtttgc agcatataag gcagaagctg cataaaggaa
    42541 acccacacat cctgaccaac catatccata ggattaatca gcgcccaccc ctgataagga
    42601 agaatggtct tcatatacca ggtcgcgaca acagtttgcg cgagcgtaag gattaacaaa
    42661 atgtaacggc ggatggtacc gacggtacgc cacttttgct cgctttcctg ctcttctttg
    42721 gtcaaacgag ccagatagcg tggcgtgaca tcgcgtccac gcagacgatc ccagaaacgg
    42781 cctaccgggt tggtacgcca cgggtcggga aacatcgagg agcgttttgc ttctggcata
    42841 gccttaagct gatcgcgtcc ttcgtcgtct ttaattaact gtccatcagc aagtgaatct
    42901 ggccaggctt gttccagacg cgcctttacc gagccttgcg gggagtcatc ctcccgcgcc
    42961 caggtacggt ggtcggcatc cagcgcctga tgaacggcgc ggatatcagt cttcggcaat
    43021 gccgctttct cgcttgcggc gatgggcatt gcgtcaatgt actcagttgt cttattcatt
    43081 ggcaggtaac tggtagctcc aggtttcact caacgtctga tcggcattca ccagcgcagc
    43141 acgcatttca gtggttttct tggcatcttt cactttcaca cgcatcacca gacgccagcc
    43201 tttggtaacc gggttatagc gcaccgtgct ttcaactatc tcaccattat caccaatgct
    43261 ggtttgcgct gtgaccgggg tatcctctgg cagttttttc atctctgcgc cggtaaaatc
    43321 gaccacaaag gcgatagtac cgtcaggttg gcgaatcagg ttcgactgct tcacatcccc
    43381 cgttgaacga cgcgtttgtt gcacccatgc gttatctggc gcatgcagtt tgtcttcatc
    43441 acggctgaag gtgatggtgt atttaaagtt catctcttta cccggctccg gcagctgatc
    43501 cggcgtccag taagcgacga tgttatcgtt ggtttcatcg ttggttggaa tttccaccag
    43561 ctcaacgctg cctttgcccc actccccttt cggagtcacc catgcgcttg gacgaagatc
    43621 gtaacgatca tcgagatctt caaagcggga gaaatcacga ccgcgctgca acagaccgaa
    43681 gccttgcggg ttttccattg agaagctgct gaccgctaaa tgtttcgggt tattcaacgg
    43741 acgccagatc cattcgccat taccagcatg gatagagaga ccgttagagt cgtgcaactc
    43801 cggacgatag ttatttgcag gcgaaggttg gttcggccca aacaggaaca tactggttaa
    43861 cggtgcaacc cccagtttgc cgactttatc gcgcagatag attttcgact gcacatccac
    43921 aaccgtgtca cgccctggca taactacgaa tttgtaagca cctgtcgcgc gcggcgagtc
    43981 aagcaatgca taaatggtta aacgtttatc agtcggtttt ggacgctcga tccagaactc
    44041 tttgaagcgt ggaaattctt cacccgatgg caaggcggta tcaattgcca ggccgcgtgc
    44101 agaaaggcca taaacctgac ctgcaccaat cacgcggaaa tagctggccc cgagcatgct
    44161 gacgatttca tcgtttttat ctttgctgtt gatcgggtaa agcactttaa aaccggcaaa
    44221 accaaggtct tttaccgtgt ctttgtcatg ctgaacatcg ccgaaagtga aataatccgg
    44281 gctgtatttg attcgtttga ctgcggtggc agtcacttca tttattttga ccggggtatc
    44341 gaagtacata ccctgatggt agaactcgag tttgaatggg gtcttcagat tgttccagta
    44401 cgctttgtca tgattaaact ggatctgctg atagtccgcg tatttcatgt cgcggaaaac
    44461 ggaggtcaag ttgcttttgg gcgcctcata gcctttcccg gctaaggatt gagcttgctt
    44521 tgcgacatca tcaatactga aagcccagct tgaagatgta tacagggtta acattactgc
    44581 agcactcaac caacgcattt tcatcatttg taatttatgt ttcataatta gtaagcactt
    44641 ccccctttgt gtgcttatat cgatccgatc tattttaatg gataaccagc cattccgaca
    44701 acagaatccc ggtatcgttc agcacgctgg ctcatggatt aagcacctga ttgcaaccgt
    44761 tttcactttc cgtgcttctc ctaaagacag aagcatcgac ttcgtgtagg gtttgccagc
    44821 aaatttattt atcaaccgtc ttacagacgc ttccattctg gttagtggta gcgatgatgc
    44881 tatgcataaa aaccttgaag cctgcgcctg gcgcggtcag gtcggtgtcg ctatggcaac
    44941 aagcctaaag gcataacccg acagggataa gacgaaaaat ccgagattag ttaaccatat
    45001 atgaatccag tacccgcgca acgtgaatat ttcctcgact ccatccgcgc ctggctgatg
    45061 ttgttaggga tcccttttca tatttcttta atctattcga gccatacatg gcatgtgaat
    45121 agcgccgaac cgtcattgtg gctgaccctt tttaatgact tcatccactc gttccgcatg
    45181 caggtatttt tcgttatatc cgggtacttt tcctacatgc tttttttacg ctatcccttg
    45241 aaaaaatggt ggaaagtacg tgtcgaacgt gtaggtatcc cgatgttaac agccatcccc
    45301 ctactgacat taccgcaatt tattatgctg caatacgtca aagggaaagc ggaaagttgg
    45361 cctggcctgt cattgtatga caaatataat acgttggcct gggaattaat atcacacctg
    45421 tggtttttac tggtgttagt agtcatgacg acgctgtgcg tatggatatt taaacgcatc
    45481 agaaataatt tagaaaattc tgataaaacg aataaaaaat tctcgatggt aaaactatcg
    45541 gtgatttttt tgtgcctcgg catcggctat gcggtaataa gaagaacgat ttttattgtg
    45601 tatccgccca ttctgagtaa cggcatgttc aattttattg tcatgcaaac gctattttat
    45661 ttgccgttct ttatcctcgg cgcactggct ttcattttcc ctcatcttaa aaccttgttt
    45721 accacgccgt ctcgtggctg tacccttgca gcagcattgg catttgtcgc ttacttactc
    45781 aaccagcgct atggcagtgg cgatgcctgg atgtacgaaa ccgagtcggt gatcaccatg
    45841 gtcctcggtc tgtggatggt gaatgtggtc ttctccttcg gccaccgttt gcttaacttc
    45901 cagtcagcgc gggtgactta ctttgttaat gcatcgctgt ttatctatct ggttcaccac
    45961 ccgttaactc tgtttttcgg cgcgtacatt acaccgcaca tcacctccaa ctggcttggt
    46021 tttctctgtg gcctgatatt cgtagtaggg attgcgataa ttctgtatga aattcattta
    46081 cgcatcccgt tactgaagtt tttgttctct ggtaaaccgg ttgttaagcg tgagaacgat
    46141 aaagcaccaa cccgttaagc cgcgtttata ataaccattc cacgggcaat atcgatgcca
    46201 gtctgaccat aacccgcttc cagaaactgg tggcgggttc ttttttgaga acaatttctt
    46261 tctttgcatg acgatcaacc cagttgatcc gtccccacct gtccagacgg agctgccagg
    46321 ccgcatcata ctggctctga ataaagcgtt tatcaattaa ctgtgccagc gtttcgctct
    46381 ctatcacgaa ccccatttca gtattgagca atgtcgaacg cggatcgaaa ttgaaagagc
    46441 cgataaacac cgttttacca tcgatgctaa aggttttagc atgcaggctg gcaccggaat
    46501 taccggttat gccgcgatcg tgtaacgtac tactttgttc acgcgtcggc ttgagttcat
    46561 ataattccac gccgtagcgg agcaattttt tgcgccagcg cgcgtatccg gcatggacga
    46621 cagcaacatc gttagcggca agagaattgg ttaggatcgc aatctttacc ccttttctca
    46681 ccatccgtaa gagttgcgcc acacctgcgc gtgtcggtac aaaataggaa gagataatat
    46741 cgatgcgttc actgggtgag cccatgatat cgaacaggcg ctgcggtaac agtgaatgcc
    46801 gttttgcctt gccctcccct ttcgccggat catcacttaa taaacgtgtt ttcgcccaga
    46861 taagcggcaa cgttccatca accagatgat ttataaacgg gctggattcc attttgcgta
    46921 aataacgatg cgtcatggca tcgttgtgcc aggatgctgg taactcgatg cgatccgcca
    46981 tttcaccttc cgggacatcc agcacctgct gtaagggtga aaccgatttg caataccagt
    47041 agcgggcgaa atcatcggca acgtcctcta caactggtcc tattgccatg acatctaaat
    47101 ccgaaaaaag tggctcttcc cctgccccaa aataggcatc accaatattt cgtcctccca
    47161 ccagggtaac cacgccatca acagtgaaac ttttattgtg catacggcga ttgagacggg
    47221 aaaagtcggt gatataacca agcggacgta gcaagcgaaa cgagaaagga ttaaaaagcc
    47281 ggacttcaat gcggggatga ctgtcaagca agcgtaaaat gtcgtcaagt ccgggtgtat
    47341 tgttgtcgtc cagcaacaaa cggacgcgaa cgccacgctt tgctgcggct aacagggcgg
    47401 aaaacagtaa ccgacccgac atatcgtcct gccagatgta atactgaaca tccagcgtat
    47461 gctctgccat ttcggccaga cgataccggg cggcaaacgc atccagactt ttctccagag
    47521 gaaaaaggcc acactgaccg ggatgttgcg aacacagtgg cagcaccgcg ctcgccagcc
    47581 ggggcaaatc attcatctcc ttgttgagta aggagtcttt cgtagaggtg ggcgttttct
    47641 tcatcataac agacaaagta tacctgttcg ggtaaagcgt gacgggtaat aaattctgaa
    47701 acggttttta ctgcgatttc agccgctgcc gcacgagggt aaccataaac cccagtacta
    47761 attgcaggaa aagccactga cgtatagctg tttgccgcca ccagtcgtag gctattgaga
    47821 taggcatcct gcaaaagctg gtcttcgttt tgttcaccac cacgccagac tggcccgacg
    47881 gtgtgcacta cggctttcgc gggaagatcg cctgcaagcg taataacggc atgccccgta
    47941 gggcaatcgc cctgctgctg cctgactttt aaacaggcat ccagcagggc cggacccgct
    48001 gcgcgatgaa tggccccatc gacgccgccg cctcccatta atgacggatt agccgcattc
    48061 acaatcacat caacggccag tttggtaata tcaccctgca caacatgaat acgcgttttc
    48121 ataacgcctc cttacacctt cttgtttctt aagtgtacag caggcttttt ggttaaatcg
    48181 catttttctg ctcagcaacg tgagttgttc agaaacttag ttatattcga tgttcattac
    48241 agccagcttt ttctgtttat caagatactg cacttttact gaggctattt gcgcgttttt
    48301 gacattgccg gtcattaacg ctttgctgga ataggttctg cccggaacag agccattctg
    48361 cgggcagttc atttctacgg gtgttgactg ggtgctgacg tcacatgccg gttccacaat
    48421 ttggccataa aagtggatca cgcccccttg cgcggtgctt cctgcgatgg caataaaagg
    48481 aaaaaacata ctgccgagca ttagagagtc taaaaaaggt ctgaacattt attctatttc
    48541 ctcaatgatt agtcatcctt gagggttgtg ttatccatac tttcaggttg gctgatccgc
    48601 agcagaccat tctctccaga ttcatcttat gctcgatatt tcaacaaatt aagacttttc
    48661 tgaagagggc ggccattgtt gtgataaatg aagtgactgt ccatcagaaa cagtaacaac
    48721 tattttcacc cgatcgtccg gggaaatatt taaactcaac ttcgtcaaag caatgggttg
    48781 attagcaggc aatgagaggg tcttttcttg cttcgtctga ctttgccctg aactgccttc
    48841 gcgcagggac aatatttgta ctctgcacag acaagattga gtaagagtga cttcaggaat
    48901 aatggtgtac atatcccctt gctgggtcgt attaaaggtt atctgactgg aaagtgccgc
    48961 aaggagtaat aacgcattca tattcttctc ccgaaaaaaa acagggcttg cgccctgttt
    49021 ctttaataca gaggatgtat tagtactgat gagcggtcgc gttgttacca aagccaacct
    49081 gagtgacgtt aacgctggag ttagatgcag tctggtcaac agcagcaccg ttaccgccac
    49141 cgaactgttt aacagtcata gtagaatctt taccattcca ctgatcaaga gtagcgctgt
    49201 tgccgaaacc acgctgagtc agatcgattg agctgtcatc agaaccttgg ccaacatctg
    49261 cgccgttacc gccgccgtgc tgggtaatgg tcagatcaga gttacgggcg tcagcttgca
    49321 gagcaagagc agagttaccg ccaccgtact ggtaaatgtt cagctctgaa tttgggccgc
    49381 tattattacc gccaccaccg tggttaccgc cgccgccgta ctgaggaaca acacctgcca
    49441 gagcgctacc agagaatacg attgctgcaa ttgctgctac ttttaaaagt ttcatgtaaa
    49501 acccccatcg gattgattta aaagtcgaat ggaaattaac gttgtgtcac gcgaatagcc
    49561 atttgcgact gtctctgcac tacaattgcc gttttttgag taccatactg tgtaatattt
    49621 gctttattac cagaaccttt ctggataatc atcgcagtat taccataagc accttgcgaa
    49681 atactggcat cgttggcatt gcccgcctga tcaatatatg caaggttata atctcctgtc
    49741 tggtcaatct ttgcccggtt gctactacct tcttgcgcaa caaccgccaa aagttttgag
    49801 cctccctgcc gtaactgagc actattatta gtcccagctt gaccaattat ggctgcctga
    49861 ttaaatgaag acttactcaa ttcatttacc gcgaagttat attctgaatt agctaaatca
    49921 taacctgctg cggctgcaat cccaggcgca cccagtattg ttaacatcat aaataacaat
    49981 ttgtttttca tgttgtcacc ctggacctgg tcgtacattt aagaaattaa atcatttcaa
    50041 cttggttgtt aacgcaacct gtattttgtt aacgctgcgt tacgatggaa agtatgtctg
    50101 cggaaatatt tttaataact cacccgcgcg ttgtattttt ctttttttgt tcataccaaa
    50161 gtattaaaac ccacgtaaat acgctgttat ctacgcaaaa aatattttgt tttcttttaa
    50221 atctccgttt tccgcaatca aaaatcacca gacagccatt cttcttaccc gtcgctgatt
    50281 gctgcgatat gtcttgcgca caagccgtga caaacctcgt ctttttgcag taaaaaattg
    50341 tccacggagg tgtgaagaaa aaacaagaac gttttacatg acgaaaggac tacaccgaaa
    50401 tattttttat atgcattatt agtaagttag caccatttgt atgatttttt aaaattgtgc
    50461 aataaaaacc aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaagatta
    50521 acatttcaat ataacgagtt acatgtttaa cacttgattt aagatttgta atggctagat
    50581 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tctattacta
    50641 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
    50701 ggtgtgcgat caataaaaaa agcggggttt catcatgttt aatgaagtcc atagtattca
    50761 tggtcataca ttattgttga tcactaaacc ttctttgcag gcgacagctc tcttgcagca
    50821 ccttaaacaa tcgctggcaa ttacaggaaa attacataat attcaacgtt ctctggacga
    50881 tatatcttca ggctctatta ttcttctgga tatgatggaa gcggataaaa agcttatcca
    50941 ttattggcag gatactttga gcaggaaaaa caacaatatc aaaatattgt tgctaaatac
    51001 gcctgaagat tacccgtacc gcgacattga aaactggcct catatcaacg gcgtttttta
    51061 tgccatggag gatcaagaac gtgttgtcaa tgggttgcaa ggcgtcctgc gcggcgaatg
    51121 ctactttacg caaaagcttg ccagttacct gattacgcat tcaggtaact atcgttataa
    51181 cagcacggaa tcagccctcc ttactcatcg ggaaaaagag atcctgaata agctgcgtat
    51241 cggcgcgtct aataacgaga tcgctcgttc gttgttcatc agcgaaaata cggttaaaac
    51301 gcatctttat aatcttttca agaagatagc cgtaaaaaac cggacacaag cggtttcctg
    51361 ggcaaacgat aacctcaggc gataaagcca tgaaacgtta tttacgctgg attgtggcgg
    51421 cagaatttct gttcgccgca gggaatcttc acgccgttga ggtagaagtc ccgggattgt
    51481 taactgacca tactgtttca tctattggcc atgattttta ccgagccttt agtgataaat
    51541 gggaaagtga ctatacgggt aacttaacga ttaatgaaag gcccagtgca cgatggggaa
    51601 gctggatcac tataacggtc aatcaggacg ttattttcca gactttttta ttcccgttga
    51661 aaagagactt cgaaaaaact gttgtctttg cactgattca aactgaagaa gcactaaatc
    51721 gtcgccagat aaatcaggcg ttattaagta cgggcgattt ggcgcatgat gaattctaaa
    51781 taaaaaattg ttcggaggct gcaatgcgtg tcaaacatgc agtagttcta ctcatgctta
    51841 tttcgccatt aagttgggct ggaacaatga cttttcagtt ccgtaatcca aactttggtg
    51901 gtaacccaaa taatggcgct tttttattaa atagcgccca ggcacaaaac tcgtataaag
    51961 atccgagcta taacgatgac tatggtattg aaacaccctc agcgttagat aactttactc
    52021 aggccatcca gtcacaaatt ttaggtgggc tactgtcgaa tattaatacc ggtaaaccgg
    52081 gccgcatagt gaccaacgat tatattgttg atattgctaa ccgcgatggt caattgcagt
    52141 tgaacgtgac agatcgtaaa accggacaaa cctcgaccat ccaggtttcg ggtttacaaa
    52201 ataactcaac cgatttttaa gccccagctt cataaggaaa ataatcatgc agcgcttatt
    52261 tcttttggtt gccgtcatgt tactgagcgg atgcttaacc gccccgccta aagaagccgc
    52321 cagaccgaca ttaatgcctc gtgctcagag ctacaaagat ttgacccatc tgccagcgcc
    52381 gacgggtaag atctttgttt cggtatacaa cattcaggac gaaaccgggc aatttaaacc
    52441 ttacccggca agtaacttct ccactgctgt tccgcaaagc gccacggcaa tgctggtcac
    52501 ggcattgaaa gattctcgct ggtttatacc gctggagcgc cagggcttac aaaacctgct
    52561 taacgagcgc aagattattc gtgcggcaca agaaaacggc acggttgcca ttaataaccg
    52621 aatcccgctg caatctttaa cggcggcaaa tatcatggtt gaaggttcga ttatcggtta
    52681 tgaaagcaac gttaaatcgg gcggtgttgg ggcaagatat tttgggatcg gtgccgacac
    52741 gcaataccag cttgatcaga ttgccgtgaa cctgcgtgtc gtcaatgtga gtaccggtga
    52801 gatcctctcc tcggtgaaca ccagtaagac gatactttcc tatgaagttc aggcgggggt
    52861 cttccgcttt attgactacc agcgcttgct ggaaggggaa gtgggttaca cctcgaacga
    52921 acccgttatg ctgtgcctga tgtcggctat cgaaacaggc gtcattttcc tgattaatga
    52981 tggtatcgac cgcggtcttt gggatttgca gaacaaagca gaacggcaga atgacattct
    53041 ggtgaaatac cgccatatgt cggttccacc ggaatcctga tagataaaaa tgccggaatt
    53101 atcatccggc attttactgt ttaagcgaaa gcgtgagtgc ccctcacgct ttatttttga
    53161 tttgctgctc acggtgagcg gtcttcgtac tacgcgccgc cagccacaag ccgctgtttt
    53221 tcattgcgta accaaatatc aacccgagcg ccagtgaagg taacaccagt ttccagtcac
    53281 cctgcccggc aaacgtcgcg catgcgccta taaacgttcc cggaacaaat gaaagcagca
    53341 gctgtttggc ctgaatacac atcagaaacg cgacaatacc ggttatgaca taaccgagaa
    53401 tttccagatg tggtgccagt gcactaccgt aaataatgac catcgcccac accacgccac
    53461 taagcagggt tgcagcggag atcgccagcc ctttcagccc accttgcggg caggcaaagt
    53521 aggccgtaca gcccaggaag cccgcccagc ttagtaagcc cagggaaaca gccacccatc
    53581 cccagatacc ggagagaatg cctgttgtga ttgcaataga gagaagtatg ttcatgccgc
    53641 gcatgatagc agaaaacgcg gccatgaatg agatcgagca cacattttat gcaacggcaa
    53701 attatctgtt gcagttatga gtgatgcaga tcacatttac tcttcagaat cttcttccag
    53761 ctcgtcccac attgcactaa tggcatcgcg ggttagcggt gccatggtgc gccagaaagg
    53821 cgtggttgca tgagcctcca ctttgccgag gaacgcacca caccagggta acagatactc
    53881 actgaacagt gtttccagtg cttcgctctc atcttccgtt gactgatctt ccagccagga
    53941 agccgcgagc agcaatgtac cgatgtgatc ggctggcgta tccgctaatg gcatccctcg
    54001 ttcggaaaga aaagcgcgca cttccgcttc cgtcgcgccc tcaacccatg cgctacgata
    54061 tggcggcaca gcacattcat cgccgataaa caacgcattg taatcggcag agacttgcgt
    54121 catatcacag cttttctgta aacgcgtcag taactcatcc tgctccagtg gccagttcgc
    54181 agccagtttc ccctcacgaa tcagggtaaa cagcggtacc agtaaaggat cttgcggttg
    54241 gcggtaatag agcgaaccca gcacacgaca gaggatagaa aactcgttca ttaatatatt
    54301 tccattacta gtaattaaag gtctgcaaat tccgcaatcg gtgccatacc gcgagattca
    54361 aggaagttca gtaagcggcg cggagaaaca ttcaaaatgc gctctggcgg aaaatctacc
    54421 gcgtcgagga ttttaagaca ctcttcaaat tcccccatgg taaacgcggt gtgagaatcc
    54481 gagcctaatg ccacccaacc tccagcgtca cgtaccgctg cagctaccgc acgacagttg
    54541 tcttcactgc ccttacgtga gtgtaaaaat gaggaattat tgatttccag cgccacctga
    54601 tgtttcgcag ctgcttcagc aacggcttta acatctattt catatttcgg atttccggga
    54661 tggcttatta tatgcacatt gccgctggcg atagtggcga tcatcgcttg tgtattggtc
    54721 gctttatcat gtggcgcaaa aaccggctca tgaaaaccgg caataatcag atccagcgaa
    54781 tcaaacattt tgccgctgca atcaatttca ccatcaacat ttttaatgtt agcttcgatg
    54841 ccgcgcagga tccctacccc atcaaccact cgcggccaga tacgcatgtt aatgaagtgc
    54901 cagtgatgtg gcgcatcttc catatccggg ccatgatcgg tgatcgcaaa aagtttaatg
    54961 cccttttgtt tggcctgggc aatgtaatca ctcaatgtgc tatatgcatg tgtgctggca
    55021 acggtatgca tatgaaggtc gacgggatac ataactctct cctgtattca attcccgcca
    55081 aggatagcag gaatcctgat gctttattag tagccgcgtg cgcggtcgac ttgcccgcag
    55141 accctctccc ctttttcgag ctgggcaata gtgcgagaaa tgtactccac agcttcagcg
    55201 ggacgggtaa tcgcggcgac atgtggtgtt atcgtcacgc gtggatgttg ccagagcgga
    55261 ctttcaggcg gtaagggttc acgattaaaa acatccagca ttgcgccttt aactttcccg
    55321 ctatccagcg ccgcgagcag gtcatcttcc acaacatgaa caccacgcgc caggttgagg
    55381 agatacgcgc catccggtaa tttttcgagt aattgttgat taataatgcc gacggtttca
    55441 ggggtattcg gtaacaaatt aatcaatacc cgacattggc tcagaaatgc agacagttct
    55501 tcccgtccag caaagctttg cacgccaggc cacgatttac gggttcgact ccagcaacgc
    55561 agcggaaagc gccaggtttg cagactctga gcaactttac tgcccagtac gcctgcgccc
    55621 aaaatgccaa tggtaaaatc ttcccgataa tattcaggca gcggttgcca atgcgaacta
    55681 ttttgctgga tgcgataatc gtcaaaacgt cgaaaccaat gcagcacctg actgacagca
    55741 tattcctgca tttgctcgcc cataccggta tcttccaggc gaaaaagtgg aacagaaggg
    55801 ttcagcattt cagggtgtgc ctgtagcttg ctcaaaatag aatcaacacc ggccccgagt
    55861 gcgaacaccg ctttaagatc gcgccctgcc agcatttcaa caggaggatg ccagactaaa
    55921 gcataatcag cagagtcatt atctccgctt ttccatgctc tgactcttgc ctgaggaata
    55981 gctttgcgca gtgcctcaat ccaccattgg gtatcgaacg ttgggtgata aaagatgata
    56041 tccatactga ctcccgaaaa gcgttgtgcg aaatttattc gcacttatcg ttatgatcta
    56101 caaagaccac cagcataaca aatccgtggt cggtggcaaa aaaagcaaat ttcgcttatt
    56161 aaaactgcat attgattgaa gtttgaataa tcgagcgatt ttttaaaaaa gtttgttgac
    56221 ctcaggtcat gatttcccta aattagcgcc cgttccagca agacaggaac
//
LOCUS       DAPOZO010000024        56009 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPOZO010000024 DAPOZO010000000
VERSION     DAPOZO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.024"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            51..127
                     /locus_tag="ROI21_001543"
     tRNA            51..127
                     /locus_tag="ROI21_001543"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:85..87,aa:Pro,seq:tgg)
     gene            274..1509
                     /locus_tag="ROI21_001544"
     CDS             274..1509
                     /locus_tag="ROI21_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDX5110482.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQIYDFLCDAGVEFIQFIPVVERLADETAVHAGLKLH
                     GPGDIQGELTEWSVHPEEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMVDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1611..3005)
                     /locus_tag="ROI21_001545"
     CDS             complement(1611..3005)
                     /locus_tag="ROI21_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HDX5110483.1"
                     /translation="MVVFLLDDVGWMDVGFNGGGVAVGNPTPDIDAVASQGLILTSAY
                     SQPSSSPTRATILTGQYSIHHGILMPPMYGQPGGLQGLTTLPQLLHDQGYVTQAIGKW
                     HMGENKESQPQNVGFDDFRGFNSVSDMYTEWRDVHVNPEVALSPDRSQYIQKLPFSKD
                     DVHAVRGGEQEAIADITPKYMEDLDQRWMEYGVKFLDKMAKSDKPFFLYYGTRGCHFD
                     NYPNAKYAGSSPARTSYGDCMVEMNDIFANLYKALEKNGQLDNTLIVFTSDNGPEAEV
                     PPHGRTPFRGAKGSTWEGGVRVPTFVYWKGMIQPRKSDGIVDLADLFPTALDLAGHPG
                     AKVANLVPKTTFIDGVDQTSFFLGTNGQSNRKAEHYFLNGKLAAVRMDEFKYHVLIQQ
                     PYAYTQSGYQGGFTGTVMQTAGSSVFNLYTDPQESDSIGVRHIPMGVPLQTEMHAYME
                     ILKKYPPRAQIKSD"
     gene            complement(2891..3265)
                     /locus_tag="ROI21_001546"
     CDS             complement(2891..3265)
                     /locus_tag="ROI21_001546"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HDX5110484.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKPVRNRTWLFSCWMMWAGWMLA
                     LTAAAWRWVTLHQISTPLPARG"
     gene            complement(3944..5140)
                     /gene="hemY"
                     /locus_tag="ROI21_001547"
     CDS             complement(3944..5140)
                     /gene="hemY"
                     /locus_tag="ROI21_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDX5110485.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(5143..6342)
                     /gene="hemX"
                     /locus_tag="ROI21_001548"
     CDS             complement(5143..6342)
                     /gene="hemX"
                     /locus_tag="ROI21_001548"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDX5110486.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLNEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQADTP
                     AAAPQGE"
     gene            complement(6364..7104)
                     /gene="hemD"
                     /locus_tag="ROI21_001549"
     CDS             complement(6364..7104)
                     /gene="hemD"
                     /locus_tag="ROI21_001549"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDX5110487.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGERDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(7101..8042)
                     /gene="hemC"
                     /locus_tag="ROI21_001550"
     CDS             complement(7101..8042)
                     /gene="hemC"
                     /locus_tag="ROI21_001550"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDX5110488.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            8429..10975
                     /gene="cyaA"
                     /locus_tag="ROI21_001551"
     CDS             8429..10975
                     /gene="cyaA"
                     /locus_tag="ROI21_001551"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDX5110489.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(11015..11335)
                     /gene="cyaY"
                     /locus_tag="ROI21_001552"
     CDS             complement(11015..11335)
                     /gene="cyaY"
                     /locus_tag="ROI21_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDX5110490.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(11458..11612)
                     /locus_tag="ROI21_001553"
                     /pseudo
     CDS             complement(11458..11612)
                     /locus_tag="ROI21_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            11801..12004
                     /locus_tag="ROI21_001554"
     CDS             11801..12004
                     /locus_tag="ROI21_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX5110491.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12041..12865
                     /gene="dapF"
                     /locus_tag="ROI21_001555"
     CDS             12041..12865
                     /gene="dapF"
                     /locus_tag="ROI21_001555"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDX5110492.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12862..13569
                     /locus_tag="ROI21_001556"
     CDS             12862..13569
                     /locus_tag="ROI21_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDX5110493.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNYTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13566..14462
                     /gene="xerC"
                     /locus_tag="ROI21_001557"
     CDS             13566..14462
                     /gene="xerC"
                     /locus_tag="ROI21_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDX5110494.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14462..15178
                     /gene="yigB"
                     /locus_tag="ROI21_001558"
     CDS             14462..15178
                     /gene="yigB"
                     /locus_tag="ROI21_001558"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDX5110495.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15262..17424
                     /gene="uvrD"
                     /locus_tag="ROI21_001559"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15262..17424
                     /gene="uvrD"
                     /locus_tag="ROI21_001559"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDX5110496.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(17469..18371)
                     /locus_tag="ROI21_001560"
     CDS             complement(17469..18371)
                     /locus_tag="ROI21_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDX5110497.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(18454..19218)
                     /locus_tag="ROI21_001561"
     CDS             complement(18454..19218)
                     /locus_tag="ROI21_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDX5110498.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPALT
                     VQAYIVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            19588..20538
                     /gene="corA"
                     /locus_tag="ROI21_001562"
     CDS             19588..20538
                     /gene="corA"
                     /locus_tag="ROI21_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDX5110499.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(20579..21070)
                     /locus_tag="ROI21_001563"
     CDS             complement(20579..21070)
                     /locus_tag="ROI21_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HDX5110500.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(21067..21915)
                     /locus_tag="ROI21_001564"
     CDS             complement(21067..21915)
                     /locus_tag="ROI21_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HDX5110501.1"
                     /translation="MNNILGSSINMPCTLFETITLFDDFSADDMQYGDMEEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEIKDLAKMFS
                     FFGQYKTLIQDLIDHFRYGNGNSFHSQELNLSFHERINKYDYNSPIRVIKECIENDIS
                     STPTIGYRPLLLQKIKIELLSSRLNKFNDFKDNFNGLGISIHDISAQKISLLNFQKYP
                     MGWSATIHFIAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIE
                     RIENYL"
     gene            complement(22226..23116)
                     /gene="rarD"
                     /locus_tag="ROI21_001565"
     CDS             complement(22226..23116)
                     /gene="rarD"
                     /locus_tag="ROI21_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDX5110502.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(23168..23635)
                     /locus_tag="ROI21_001566"
     CDS             complement(23168..23635)
                     /locus_tag="ROI21_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDX5110503.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            23800..24669
                     /gene="pldA"
                     /locus_tag="ROI21_001567"
     CDS             23800..24669
                     /gene="pldA"
                     /locus_tag="ROI21_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDX5110504.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYQITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            24802..26631
                     /gene="recQ"
                     /locus_tag="ROI21_001568"
     CDS             24802..26631
                     /gene="recQ"
                     /locus_tag="ROI21_001568"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDX5110505.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            26695..27315
                     /gene="rhtC"
                     /locus_tag="ROI21_001569"
     CDS             26695..27315
                     /gene="rhtC"
                     /locus_tag="ROI21_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDX5110506.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHFIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(27377..27997)
                     /gene="rhtB"
                     /locus_tag="ROI21_001570"
     CDS             complement(27377..27997)
                     /gene="rhtB"
                     /locus_tag="ROI21_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDX5110507.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            28108..29130
                     /gene="pldB"
                     /locus_tag="ROI21_001571"
     CDS             28108..29130
                     /gene="pldB"
                     /locus_tag="ROI21_001571"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDX5110508.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQHHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDTTPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            29138..29938
                     /gene="yigL"
                     /locus_tag="ROI21_001572"
     CDS             29138..29938
                     /gene="yigL"
                     /locus_tag="ROI21_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDX5110509.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            30014..30913
                     /gene="bioP"
                     /locus_tag="ROI21_001573"
     CDS             30014..30913
                     /gene="bioP"
                     /locus_tag="ROI21_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDX5110510.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWIGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(30801..31754)
                     /gene="metR"
                     /locus_tag="ROI21_001574"
     CDS             complement(30801..31754)
                     /gene="metR"
                     /locus_tag="ROI21_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDX5110511.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            31872..34133
                     /gene="metE"
                     /locus_tag="ROI21_001575"
     CDS             31872..34133
                     /gene="metE"
                     /locus_tag="ROI21_001575"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDX5110512.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPIYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDTYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRGDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(34622..34879)
                     /locus_tag="ROI21_001576"
     CDS             complement(34622..34879)
                     /locus_tag="ROI21_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDX5110513.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSRPRYRVSQYGLNLIKEQQDNLF"
     gene            complement(34876..36009)
                     /gene="hcp"
                     /locus_tag="ROI21_001577"
     CDS             complement(34876..36009)
                     /gene="hcp"
                     /locus_tag="ROI21_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDX5110514.1"
                     /translation="MSDIIYLNITGEQQGCISSRCGTSASIGNRWQIGHEDEIFAFSL
                     SNSITNTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYY
                     NIQLRGALLSAINHLFTENNLDTETITVSYEYILSRHLIANTEFSYLAFPDNYNRLFI
                     PRPKTKDDNGLKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNK
                     GLLIAGASIVAGVVLGRMRFPDLESLEHFGARGAVSGRPFNPDLAGGPIENLTIDGVT
                     INREGIAIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYC
                     NLGWETGEPKGLDGYELWNNVHTATLEDFKIKDTDLFHPDATK"
     gene            complement(36401..37213)
                     /locus_tag="ROI21_001578"
     CDS             complement(36401..37213)
                     /locus_tag="ROI21_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDX5110515.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQDIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRN"
     gene            37475..38236
                     /gene="udp"
                     /locus_tag="ROI21_001579"
     CDS             37475..38236
                     /gene="udp"
                     /locus_tag="ROI21_001579"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDX5110516.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            38377..39804
                     /gene="rmuC"
                     /locus_tag="ROI21_001580"
     CDS             38377..39804
                     /gene="rmuC"
                     /locus_tag="ROI21_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDX5110517.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            39899..40654
                     /gene="ubiE"
                     /locus_tag="ROI21_001581"
     CDS             39899..40654
                     /gene="ubiE"
                     /locus_tag="ROI21_001581"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDX5110518.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            40668..41273
                     /gene="ubiJ"
                     /locus_tag="ROI21_001582"
     CDS             40668..41273
                     /gene="ubiJ"
                     /locus_tag="ROI21_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDX5110519.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            41270..42910
                     /gene="ubiB"
                     /locus_tag="ROI21_001583"
     CDS             41270..42910
                     /gene="ubiB"
                     /locus_tag="ROI21_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDX5110520.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            42989..43258
                     /gene="tatA"
                     /locus_tag="ROI21_001584"
     CDS             42989..43258
                     /gene="tatA"
                     /locus_tag="ROI21_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDX5110521.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            43262..43777
                     /gene="tatB"
                     /locus_tag="ROI21_001585"
     CDS             43262..43777
                     /gene="tatB"
                     /locus_tag="ROI21_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDX5110522.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            43780..44556
                     /gene="tatC"
                     /locus_tag="ROI21_001586"
     CDS             43780..44556
                     /gene="tatC"
                     /locus_tag="ROI21_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDX5110523.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            44598..45380
                     /gene="tatD"
                     /locus_tag="ROI21_001587"
     CDS             44598..45380
                     /gene="tatD"
                     /locus_tag="ROI21_001587"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDX5110524.1"
                     /translation="MFDIGVNLTSSQFAKDRDDIVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(45377..45865)
                     /gene="rfaH"
                     /locus_tag="ROI21_001588"
     CDS             complement(45377..45865)
                     /gene="rfaH"
                     /locus_tag="ROI21_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDX5110525.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKMVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            46032..47525
                     /gene="ubiD"
                     /locus_tag="ROI21_001589"
     CDS             46032..47525
                     /gene="ubiD"
                     /locus_tag="ROI21_001589"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDX5110526.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIISGDDVDLHRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            47571..48272
                     /gene="fre"
                     /locus_tag="ROI21_001590"
     CDS             47571..48272
                     /gene="fre"
                     /locus_tag="ROI21_001590"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDX5110527.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(48455..49618)
                     /gene="fadA"
                     /locus_tag="ROI21_001591"
     CDS             complement(48455..49618)
                     /gene="fadA"
                     /locus_tag="ROI21_001591"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDX5110528.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     NREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(49628..51817)
                     /gene="fadB"
                     /locus_tag="ROI21_001592"
     CDS             complement(49628..51817)
                     /gene="fadB"
                     /locus_tag="ROI21_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDX5110529.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKSAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYSGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDTIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            52007..53338
                     /gene="pepQ"
                     /locus_tag="ROI21_001593"
     CDS             52007..53338
                     /gene="pepQ"
                     /locus_tag="ROI21_001593"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDX5110530.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGRFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            53338..53952
                     /locus_tag="ROI21_001594"
     CDS             53338..53952
                     /locus_tag="ROI21_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDX5110531.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            53991..55442
                     /gene="trkH"
                     /locus_tag="ROI21_001595"
     CDS             53991..55442
                     /gene="trkH"
                     /locus_tag="ROI21_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDX5110532.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVIICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            55454..55999
                     /gene="hemG"
                     /locus_tag="ROI21_001596"
     CDS             55454..55999
                     /gene="hemG"
                     /locus_tag="ROI21_001596"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDX5110533.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT        13230 a        14041 c        14819 g        13919 t
ORIGIN      
        1 tcgcaccacg actttaaaga attgaactaa aaattcaaaa agcagtattt cggcgagtag
       61 cgcagcttgg tagcgcaact ggtttgggac cagtgggtcg gaggttcgaa tcctctctcg
      121 ccgaccaatt ttgaaccccg cttcggcggg gttttttgtt ttctgtgcat ttcgtcactt
      181 tcccttcgca ataaacgccc gtaataactc attgcgccac ggtatgattt cgcccttaac
      241 ttattgaagg atgacttcag gcaaggagcg accatgctgc aacaggttcc aacgcgtgct
      301 tttcatgtga tggcgaaacc gagcggttcc gattgtaatc tgaactgtga ctactgtttt
      361 tatctcgaaa aacaatccct ttaccgcgaa aagccagtca cgcatatgga cgatgacacg
      421 ctggaagcgt atgtccgtca ctatatcgct gccagcgaaa cccaaaacga agtggctttt
      481 acctggcagg gcggcgaacc aacgttactc gggctggatt tttaccgccg agccgtggcg
      541 ctacaggcga aatatggtgc tggcaggaag ataagtaaca gcttccagac taacggcgtt
      601 ctgcttgatg acgaatggtg tgcatttctg gcagaaaatc attttcttgt tgggttatcg
      661 ctggatggtc cggctgagat ccataatcaa tatcgcgtga ccaaaggcgg cagaccaacg
      721 cataagctgg tgatgcgtgc cctgacgctc ctgcaaaaac atcatgtcga ctataacgtg
      781 ctggtctgcg taaatcgcac cagtgcgtta caacctttgc aaatctatga ttttctgtgt
      841 gatgcaggcg ttgagtttat ccagtttatt cctgtggtcg aacgcctggc tgatgaaacg
      901 gctgttcatg ctggacttaa gctacatggt cccggcgata ttcagggcga actgacggaa
      961 tggtcggtac accccgagga gttcggtgaa tttttggtgg cgatattcga ccactggatc
     1021 aaacgcgacg tcggcaagat tttcgtgatg aatatcgaat gggcgtttgc caattttgtc
     1081 ggtgcgccag gagcggtttg ccatcatcag ccaacctgtg ggcgctcggt gattgttgaa
     1141 cacaacggcg acgtttacgc ctgcgatcac tatgtttatc cgcaatatcg actggggaat
     1201 atgcttcagc agacaattgc agaaatggta gattccccgc aacagcaggt gtttggtgaa
     1261 gataaattta agcagttacc ggcgcagtgt cgcagttgta atgtgttaaa agcgtgctgg
     1321 ggaggctgtc cgaaacaccg ctttatgctc gatgccagcg gcaaaccggg actgaattat
     1381 ttgtgtgccg ggtatcagcg ttatttccgc catctaccgc catatctaaa agcaatggct
     1441 gatttgctgg cgcacggtcg tccggccagc gacattatgc aggcgcattt gatggtggtg
     1501 aataagtaaa aaagcggcca ttttgtggcc gcctgcggtt gattgccgga taaggcgttc
     1561 acgccgcatc cggcaaggtc agtgtggccc gatgtagcga tagcgccggc ttaatcagat
     1621 ttaatctgcg cgcgtggtgg atattttttc aggatctcca tatacgcgtg catttcggtc
     1681 tgtagcggta cacccattgg gatatggcgc acgccgatag agtcgctttc ctgcggatcg
     1741 gtgtacaggt taaacaccga cgatcccgcc gtttgcatta ctgtgccggt gaatccaccc
     1801 tgatacccgc tctgggtata cgcgtacggt tgctggatca gtacgtgata cttgaactca
     1861 tccatacgca cagcagcgag tttaccgttg aggaagtagt gctcggcctt acggttagac
     1921 tgaccatttg ttcccaggaa gaaagatgtc tggtccacac catcgataaa ggtggttttc
     1981 ggtactaaat tcgctacttt cgccccagga tgccccgcca gatccagtgc cgtagggaag
     2041 agatccgcca gatcgacaat gccgtcagat ttacgcggtt ggatcatgcc tttccagtaa
     2101 acgaaggttg gcacacgaac gccgccttcc catgtagaac ctttcgcacc gcggaacggc
     2161 gtgcgaccgt gcggcggaac ttcggcttcc ggaccgttat cagaggtgaa cacaatcagc
     2221 gtgttatcaa gctgaccgtt tttctccagt gctttataca gattggcgaa gatatcgttc
     2281 atctccacca tgcagtcgcc gtaagaggtg cgcgccggag agctacccgc atatttcgca
     2341 tttgggtagt tatcgaagtg acagccgcga gtgccgtagt agaggaagaa aggcttatcg
     2401 ctcttcgcca tcttgtcgag gaacttaacg ccatattcca tccagcgttg atccaggtct
     2461 tccatatatt tcggcgtaat gtcggcaatg gcttcctgtt caccgccgcg cacggcatga
     2521 acgtcatctt tgctgaacgg cagtttttgg atgtattgag aacgatccgg gctcagggcg
     2581 acttccgggt tgacgtgaac atcacgccat tcggtataca tatcggatac cgagttaaag
     2641 ccgcggaaat catcaaagcc aacgttctgc ggctgcgact ctttgttttc ccccatatgc
     2701 catttcccga tggcctgagt gacgtagccc tgatcgtgca gcaactgcgg cagagtggtt
     2761 aacccttgca gaccgcccgg ttgcccgtac attggcggca tcagaatgcc gtggtggatg
     2821 gagtattgtc cggtaagaat cgtggcgcgg gttggggaag aactcggttg agaatacgcc
     2881 gaagttaaaa tcagcccctg gctggcaacg gcgtcgatat ctggtgtagg gttacccacc
     2941 gccacgccgc cgccgttaaa gccaacatcc atccagccca catcatccag caagaaaaca
     3001 accacgttcg gtttcttacc ggttttttct caagttctgc cagcttctgc tgggtttctt
     3061 tgtcttgtgc aggatgctgc atcaccggca tcatgttgtc ggcaatagtg gtcgccggtt
     3121 taaccagata ctggtttggg tgatcgtatc cggcaaagcc tttgcgtgcg gtggcggttg
     3181 acggggtatc tgctgcgttg gccatgagtg gaagagcggc ggcgacagcc acaacaagaa
     3241 gtttgggtga aaacgaaaat tccatgcaaa atgctccggt ttcatgtcgt caaaatgttg
     3301 acgtaattaa gcattgataa ttgagatccc tctccctgac aggatgattg cataaataat
     3361 agtgatgaaa ataaattatt tatttatcca gataatgaat tggaaaatca ggagagcgtt
     3421 ttcaatccta cctctggcgc aggtgatatg taaggcggtg atgttatatc gcgttgatta
     3481 ttgatgctgt ttttagtttt aacggcaatt aatatatatg ttattaattg aatgactttt
     3541 attattcatt atatatatgt gtagaatcgt gcgcaggaga aatattcact caggaagtta
     3601 ttactcagga agcaaagagg attacagaat tatctcataa caagtgttaa gggatgttat
     3661 ttcccggttc tctgtggcat aataaacgag tagatgctca ttccatctct tatgttcgcc
     3721 ttagagcctc ataaactccg gaatgacgca gagccgttta cggtgcttat cgtccactga
     3781 cagatgtcgc ttatgcctca tcagacacca tggacacaac gttgagtgaa gcacccactt
     3841 gttgtcatac agacctgttt taacgcctgc tccgtaataa gagcaggcgt ttttttatgt
     3901 atcagaaagg tcccggagga gcttgcctcc gggtgagaaa gaactactgt ggcgggttgt
     3961 tctgcaacgt taacatcaaa ccgtcgcggc gcatcgctgc agcttcttct ggtttgtgca
     4021 gtctgtccag cgcgtcggca agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg
     4081 ctgcgcggaa ggcgagcgat gcttcctgcc attctccgtg cttcatcagc gactgaccca
     4141 gtgtgctcca caacagcggg cgatcgccga cgtttttgat ttgctggcgc agcacttttt
     4201 caagctgttc cgggttattg gttttcagac gcgggatcgg cagcagtagg cgatcgtcat
     4261 actggcgttt caggccgtcg atgataattt gctgggcagt atcatgatcg tcacattcaa
     4321 taagatgttc cgccattgcc acctgcaacg ccacctgatg acgcgttttc cggctttggt
     4381 ttttccacca gttacgcaaa ccttcgctac cgttatcggc acgcgcctga tccatcaggc
     4441 caatccatgc ctgttgttcc agcattgcac gatgttcttc atcaccaaca tgggctttcg
     4501 ccattgatgg gatgatatcc agcagcgaac tccatgcacc tgtgcggata tacgcctgtt
     4561 ccgccagacg taatacttcc ggatggcgtg gcgtaacttc cagcagctta tccacgccgt
     4621 ggcgtgcagc atggttttca ttacgggcca gttgcagacg tacacgggtg atttctaccg
     4681 gaatggtgtc attgccggcc agctccgctg cgcgttccag atgttggttg gcgcgtgctt
     4741 catcaccacg ttgttgcgcg gcttcggcag ccagtagata gttcaccacc ggttgttcag
     4801 cgtgatcggc atttttcgcc atcagctttt caacttgctg ataatcgcct tccgctagtt
     4861 tcagcagcgc ctgttcggtc tgcttacggg cacggcgacg cttacgtccg acaaaccacc
     4921 cacgggtgtg tgcgcctgtg cggaagatcc gccgcagtag ccactcaatg gcaaacagca
     4981 ctaccatcgc cagaatcaaa ataatcgcca ggccagtgac gctggtttca atattgtagt
     5041 tgtcggtctg aatcaatacg taaccctgat gaccggcaat catcgggcca accactatcc
     5101 ccgcaatcag caaaacaaag agcaataaca cttttagcat ggttattctc cttgcggcgc
     5161 ggctgccgga gtatcagctt gcggtgcagg tgcaggtgca ggtgcaggtg caggtttagc
     5221 ttccgttgtc cccgctgccg gttgtgccaa caggttacgc acgcgagtct gcatcagttt
     5281 ttccagcatc gcctggcttt gcagggtttc cgggagatcc atcgagatat tttgctggct
     5341 taactggtcc acctcgttga ggaacgcttt ggtggtggca tcatcagtat cgtagtaagc
     5401 acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct gatgacgcgg
     5461 tacggcttgt gccgcgacca gcaggcgaga gcgaatattt tcgcgcagat agatatcctg
     5521 atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa tgaagttgtc
     5581 cataaagttc tgccagcttt tttgtagatt gatacgccat tcgctgatgg aactggaaag
     5641 ctcttcaccg tcggaatcca tcggcgaacc atcgctgtca ttatcggcca gacgcaggtt
     5701 atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat ccacctgcga
     5761 taccgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa tcagactcgg
     5821 gtcattcata tccgccaggc tggcatctgc acttttcagc aacgctgcag cggtcgtgac
     5881 gtcttgatcg ctccacagct tccgtcctgc gagtttcacc agaaaatcgg cctgagccag
     5941 cagccaggtt ttagcatcgc tgccggaaat ggtggcgacc ttttgttgga cttcatccaa
     6001 ctgttttgcc agcgtttctt gctgacgatt cgcctgctca agctgtacag cttgttgctt
     6061 gataatgcct tccagctcag ctttttggct ctcctgggct ttttgcagtg ccgtcagttg
     6121 attagccagg gcatcgctgg tggcagtctg attgacggcc tgttgtttac cccagccata
     6181 caaaccgacg cccgccgcca gagcaatagc aatagccacc gcgctgagaa tcaatgcggt
     6241 attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca cggcctccct
     6301 ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt cctgttatga
     6361 gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga ccttaatgtc
     6421 ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca atagtcgaca
     6481 gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt gctgcaacat
     6541 ttcaccgctg gtaacaacga ccgtcgtcac ctcgcgggat tgccagcgca tcgcttcttc
     6601 tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga cctcagcacc
     6661 gcgcgccgtc agggtatccc caattagctc acgaccgcca ttgccacgta atatcagcgc
     6721 acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc tgatttcccg
     6781 atcctgcggg tagagaatct tctgtccgct tacggtatgt agcgccagtg cggtggtgcg
     6841 tccaatggcg aaataatcag gtagtcgggg ccatttacga tcttgctgat gcagctgtga
     6901 ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatccctct cccccagcgc
     6961 tgcaagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa tcagcggaaa
     7021 atgccaggcc acctgcccca gtgtgcgcag acggctcact aactcttctc cagcgggaga
     7081 cgggcgggtg accaagatac tcatgccggg gcgtctccgt tatagacttc agcaaggatc
     7141 tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg ttcggcatct
     7201 tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc gccgaccagc
     7261 gcacgcagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg tacctgacat
     7321 ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc agtttcgtgg
     7381 tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca ttcaatgccc
     7441 accgcacctt gtcctaccgc cggaagagaa atctcgggtg gcaacgcggc gcgaatgcgt
     7501 gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc gtattcgccg
     7561 ttatccagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat aatcagatcc
     7621 gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac gatactgcct
     7681 gccggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg aggatcttca
     7741 cgctcacaaa tagtgaccag tcccagacct tgcgggaatt caaccggcac atctttcatt
     7801 gaatgtacgg cgatatcggc gcgattttcg aggagcgcga cttccagctc ttttacaaat
     7861 aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc gcgcgtcacc
     7921 atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc tttgacatag
     7981 tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac attgtctaac
     8041 atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca gagcgatgtc
     8101 agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg gcagtggatc
     8161 ctgacaggag tttcacgccg tcgtaataag gaatttacag agaataaacg gtgctacact
     8221 tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga tcacgtttta
     8281 gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa cggttacctt
     8341 tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga atcacagtca
     8401 tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc tgaaacagag
     8461 actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg ggcctgcatt
     8521 ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc taatgccggg
     8581 ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg aaactcaacg
     8641 ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc cgccgaaagg
     8701 tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag ggcaaagttg
     8761 ttcctctgac ctggatatct gggtctgtca tcaatcctgg cttgatagcg aagagcgcca
     8821 gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg gcgtggaagt
     8881 tagcttcttc ctgattgatg aaaatcgctt ccgtcataat gaaagcggca gcctgggggg
     8941 cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttacc gtaccgccgt
     9001 gcgtctcgct ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag aagagcatta
     9061 cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg aatggctgga
     9121 tctcggtggt ttaagctcac tttctgctga agagtacttt ggtgccagcc tttggcagct
     9181 ctacaagagt attgattccc catacaaagc ggtgctgaaa acactgctgc tggaagccta
     9241 ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc gtttgcacga
     9301 cggcgagatt gtatcgtttg gcctcgaccc gtactgcatg atgctggagc gtgttactga
     9361 atacctgacg gcgattgaag atttcacccg tctggattta gtgcgtcgct gcttctattt
     9421 aaaagtgtgc gaaaagctca gccgtgaacg cgcctgcgta ggctggcgtc gtgaagtgct
     9481 aacccagtta gtgaaagagt gggaatggga cgatgctcgt ctggcaatgc tcgataaccg
     9541 cgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg atgcaatgat
     9601 gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aacctgagcg tctccgccag
     9661 tccgcaggat atcggcgtgc tgacgcgtaa actgtacgcc gcgtttgaag cattgccagg
     9721 taaagtgacg ctggtaaacc cgcagatttc gcccgatctc tcggaaccga atctgacctt
     9781 tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata accgcgcgcc
     9841 aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc tgaataaact
     9901 ggtggcgtgg gcatggttta acggcctgct gacctcgcgt acccgcttgt atattaaagg
     9961 caacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgatgtgt cacaccattt
    10021 cccgctgcgc ctgcctgcac cgacgccaaa agcgctctac agcccgtgtg aaattcgcca
    10081 tctggcgatt atcgttaacc tggaatatga cccgacagct gcgttccgca atcaggtggt
    10141 gcatttcgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa attgcctggt
    10201 aggtagcgtg gacctgctgt atcgcaactc gtggaacgaa gtgcgtacgc tgcacttcaa
    10261 cggcgagcag tcgatgatcg aagccctgaa aactattctc ggcaaaatgc atcaggacgc
    10321 cgcaccgcca gatagcgtgg aagtcttctg ctacagccag catctgcgcg gattaatccg
    10381 tactcgcgtg cagcaactgg tttctgagtg cattgaactg cgtctttcca gcacccgcca
    10441 ggaaaccggg cgcttcaagg cgctgcgcgt ttctggtcaa acctggggct tattcttcga
    10501 gcgcctgaat gtatcggtac agaaactaga aaatgccatc gagttttatg gcgctatttc
    10561 gcataacaaa ctgcacggcc tgtcagtgca ggttgaaacc aatcacgtca aattaccggc
    10621 ggtggtggac ggctttgcca gcgaagggat catccagttc ttcttcgaag aaacgcaaga
    10681 cgaaaatggc tttaatatct acattctcga cgaaagcaac cgggttgagg tgtatcacca
    10741 ctgcgaaggc agcaaagagg agctggtgcg tgacgtcagt cgcttctact cgtcatcgca
    10801 cgaccgtttt acctacggct caagcttcat caacttcaac ctgccgcagt tctatcagat
    10861 tgtgaaggtt gatggtcgtg aacaggtgat tccgttccgc accaaatcga ttggcaacat
    10921 gccgcctgcc aatcaggatc acgacacgcc gctattacag cagtattttt cgtgatgagc
    10981 gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact gtttcacccg
    11041 cttgctgcgt tgccgcctgt tccagcaaat cccagaaggt ttcgccgcta cgatcgcaaa
    11101 tccactcatc gcctttcaga tcaaaatggt agccgccctg tttggttgcc agccatacct
    11161 ggtgcagcgg ttcctggcgg ttgataatga ttttgctgcc gttctcaaag gtaatggtta
    11221 gtacaccgcc gttgatttca cagtcgatat cgctgtcgcc atcccagtcg tccagacgct
    11281 cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg ttcattgttg
    11341 tatcctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct tcaaagtttg
    11401 ctcgtctgct gcgttctggc atgattcatc cgtcaacgga ataataatgt ctctatatag
    11461 cgaaagattg gtcttttcat aagggcgcaa atacaccacg ccttttccct gtttctggta
    11521 aatattatgt tccgctcatc aagccgacat ccccatgccg acgataaaaa caacgaacaa
    11581 gccaacaaac aaaccacatt gcgatagtgc ataaagccat cctggcgcga ggtgccgatc
    11641 acgaaactac cagcaaaaca taaatcgcca cgagtaagcg ttatactcgc agcatttcct
    11701 cacttttcag acttcataaa gagtcgctaa acgcttgctt ttacgccttc tcctgcgatg
    11761 atagaaagca gaaagcgatg aactttacag gcaatccata atgaaaaacg tgtttaaggc
    11821 actcactgta ttacttactc tcttcagcct gacgggctgc ggtctgaaag gtccgctcta
    11881 tttcccgcct gcagataaaa acgcaccgcc gccgaccaaa ccggtagaga cgcaaacgca
    11941 atccacggtg ccggataaaa acgaccgcgc cactggcgat ggtccatccc aggtgaatta
    12001 ctaaaagtca gtttctgtac ccgcgtgatt ggagtaaatg atgcagttct cgaaaatgca
    12061 tggccttggc aacgatttta tggtcgtcga cgcggtaacg cagaatgtct ttttttcacc
    12121 ggagctgatt cgtcgcctgg cggatcggca cctgggggta gggtttgacc agctgctggt
    12181 ggttgagccg ccgtatgatc ctgaactgga ttttcactat cgcattttca atgctgatgg
    12241 cagtgaagtg gcgcagtgcg gcaacggcgc gcgctgcttt gcccgttttg tgcgtctgaa
    12301 agggctgacc aataagcgcg atatccgcgt cagcaccgcc aacgggcgga tggttctgac
    12361 cgtcaccgat gatgatctgg ttcgcgtaaa tatgggcgag cccaacttcg aaccttccgc
    12421 tgtgccgttt cgcgctaaca aagcggaaaa gacctatatt atgcgcgccg ccgagcagac
    12481 aatcttatgt ggcgtggtgt cgatgggaaa tccgcactgc gtgattcagg tcgatgatgt
    12541 cgatactgcg gcggtagaaa cgcttggtcc tgttctggaa agccatgagc gttttccgga
    12601 gcgcgccaat atcggtttta tgcaagtggt taagcgcgag catattcgtt tacgcgttta
    12661 tgagcgtggg gcaggagaaa cccaggcctg cggcagcggc gcatgtgcgg cggtggcggt
    12721 agggatccag caaggtttgc tggccgaaga agtacgcgtg gaactccccg gcggtcgtct
    12781 tgatatcgcc tggaaaggtc cgggtcaccc gttatatatg actggcccgg cggtacatgt
    12841 ctacgacgga tttattcatc tatgaagcaa ccaggggaag aactgcagga aacactcacg
    12901 gagcttgatg accgggcggt tgtcgattat ctgattaaaa atcctgagtt ttttatccgc
    12961 aatgcgcgcg cagttgaagc gatacgtgtg ccgcatccgg tacgcggcac cgtttcgttg
    13021 gtcgaatggc acatggcccg tgcacgtaat catattcatg ttctggaaga gaacatggcg
    13081 ttgttgatgg aacaggctat cgccaacgaa gggctgtttt atcgcttact ctacttgcag
    13141 cgcagtctca ccgccgccag cagtctcgat gatatgctga tgcgctttca ccgctgggcg
    13201 cgcgatctcg gcctggcggg tgcgagtctg cgcctgttcc cggatcgctg gcgtttaggt
    13261 gcaccgtcga actacactca tctggcatta agccgtcagt ctttcgaacc gctgcgtatt
    13321 cagcgtctgg ggcaggaaca gcactatctt gggccgctta acgggccgga gttgctggtg
    13381 gtgctacctg aagcgaaagc ggtgggatcg gtggcgatgt cgatgctggg aagcgatgct
    13441 gatttgggtg ttgtgctgtt taccagtcgc gatgccagtc actatcaaca agggcaggga
    13501 acgcagttac ttcatgaaat tgcgctgatg ttgccggagc ttctggagcg ttggattgaa
    13561 cgcgtatgac cgatttacac accgatgtag aacgctacct acgttatctg agcgtggagc
    13621 gccagctaag cccgataacc ctgcttaact accagcgtca gcttgaggcg atcatcaatt
    13681 ttgccagcga aaacggcctg caaagctggc agcaatgtga tgtgacgatg gtgcgcaatt
    13741 tcgctgtacg cagtcgccgt aaagggctgg gagctgcaag tctggcgtta cggctttctg
    13801 cgctacgtag cttttttgac tggctggtca gccagaacga actcaaagct aacccggcga
    13861 aaggtgtttc ggcaccgaaa gcgccgcgtc atctgccgaa aaacatcgac gtcgacgata
    13921 tgaatcggct gctggatatt gatatcaacg atcccctcgc cgtgcgcgat cgggcaatgc
    13981 tggaagtgat gtacggcgcg ggtctgcgtc tttccgaact ggtggggctc gatatcaaac
    14041 acctcgacct ggagtctggt gaagtgtggg tgatggggaa aggcagcaaa gagcgccgcc
    14101 tgccgattgg tcgcaacgct gtggcgtgga ttgagcactg gcttgatttg cgcgacctgt
    14161 ttggtagcga agacgacgcg ctttttctgt cgaaactggg caagcgtatc tccgcgcgta
    14221 atgtgcagaa acgctttgcc gaatggggca taaaacaagg gctgaataat cacgttcacc
    14281 cgcataaatt acgtcactcg ttcgctacgc atatgctgga gtcgagcggc gatcttcgtg
    14341 gtgtgcagga gctgctgggt catgccaacc tctccaccac gcaaatctat actcatcttg
    14401 attttcaaca ccttgcctcg gtgtacgatg cggcgcatcc acgcgccaaa cgggggaaat
    14461 aatgcgtttt taccggcctt tggggcgcat ctcggcgctc acctttgacc tggatgatac
    14521 cctttacgat aaccgtccgg tgattttgcg caccgagcga gaggcgctta cctttgtgca
    14581 aaattatcat ccggcgctgc gcagcttcca gaatgaagat ctgcaacgcc tgcgccaggc
    14641 ggtacgggaa gcggaacccg agatttatca cgacgtgacg cgctggcgtt ttcgttcgat
    14701 tgaacaagcc atgctcgacg ccgggctgag tgccgaagaa gccagtgcag gcgcacacgc
    14761 ggcaatgatc aactttgcca aatggcgcag ccgaatcgat gtcccgcagc aaacccacga
    14821 caccttaaaa cagctggcga agaaatggcc gctggtggcg atcaccaacg gtaacgccca
    14881 gccggaactg tttggtttgg gggattattt tgagtttgtg ctgcgcgctg gcccgcacgg
    14941 gcgctcaaaa ccgttcagcg atatgtattt tctggctgcg gaaaaactca acgtgccgat
    15001 cggcgagatc ttacatgttg gggacgatct caccactgac gtgggtgggg caattcgcag
    15061 cggaatgcag gcttgttgga tcagaccgga aaatggcgat ctgatgcaaa cctgggacag
    15121 ccgtttactc ccgcatctgg aaatttcccg gttggcatct ctgacctcgc tgatataatc
    15181 agcaaatctg tatatatacc cagctttttg gcggagggcg ttgcgcttct ccgcccaacc
    15241 tatttttacg cggcggtgcc aatggacgtt tcttacctgc tcgacagcct taatgacaaa
    15301 cagcgcgaag cggtggccgc gccacgcagc aaccttctgg tgctggcggg cgcgggcagt
    15361 ggtaagacgc gcgtactggt gcaccgtatc gcctggctga tgagcgtgga aaactgctcg
    15421 ccatattcga ttatggcggt aacgtttacc aacaaagcgg cggcggagat gcgtcatcgt
    15481 atcgggcagc tgatgggcac cagccagggc ggcatgtggg tcggcacctt ccacgggctg
    15541 gcgcaccgtc tgctgcgtgc gcaccatatg gacgccaatc tgccgcagga ttttcagatc
    15601 ctcgacagcg aggaccagct acgcctgctc aaacgcctaa tcaaggcgat gaacctcgac
    15661 gagaagcagt ggccgccgcg ccaggcaatg tggtacatca acagccagaa agatgaaggc
    15721 ctgcgtccac atcatattca aagctacggt aatccggtgg agcagacctg gcagaaggtg
    15781 tatcaggcgt atcaggaagc gtgtgaccgc gcgggcctgg tggacttcgc cgagctgctg
    15841 ctacgtgctc acgagttgtg gcttaacaag ccgcatatcc ttcagcacta tcgcgaacgg
    15901 tttaccaata tcctggtgga cgaattccag gataccaaca acattcagta tgcgtggatc
    15961 cgcctgctgg cgggcgatac cggcaaagtg atgatcgttg gcgatgacga tcagtcaatc
    16021 tacggctggc gtggagcgca ggtggagaat attcagcgtt tcctcaatga tttccccggt
    16081 gctgaaacta tccgtctgga gcagaactac cgctctacca gcaatattct gagcgccgcg
    16141 aacgccctga ttgaaaacaa caacgggcgt ttgggtaaaa aactgtggac cgatggcgcg
    16201 gatggtgagc ctatttccct ctattgcgct tttaacgaac tcgatgaagc gcgttttgtg
    16261 gttaaccgta tcaaaacctg gcaggacaac ggcggggcgc ttgccgagtg cgccattctc
    16321 taccgcagca acgcccagtc gcgtgtgctg gaagaagcac tgttacaggc aagtatgccg
    16381 taccgtattt acggcgggat gcgattcttc gaacgccagg aaatcaaaga tgcgctctcg
    16441 tatctgcgcc tgattgccaa ccgtaatgac gacgcggcct ttgagcgcgt ggtgaataca
    16501 ccaacgcggg gtattggtga ccggacgctg gacgtggtac gtcagacatc gcgcgatcgc
    16561 cagttaacac tctggcaggc atgtcgtgaa ctgttgcagg aaaaagccct cgccggacgt
    16621 gctgccagtg ccttacagcg gtttatggaa ctgatcgacg ccttagcgca ggaaactgcc
    16681 gatatgccgc tgcatgtaca gactgaccgg gtaattaaag actccggcct gcgcacaatg
    16741 tacgagcagg agaaaggcga aaaaggccag acgcgtatcg aaaacttaga ggaactggtg
    16801 acggcgacgc gccagttcag ctacaacgaa gaagacgaag atttaatgcc gctgcaggcg
    16861 ttcctctccc atgcggcact ggaagcgggc gaggggcagg cggatacctg gcaggatgcg
    16921 gtgcagttga tgacgctaca ctcggcgaaa ggtctggagt tcccgcaggt gtttatcgtc
    16981 ggtatggaag agggcatgtt cccaagccag atgtcgctgg atgaaggtgg gcgtctggaa
    17041 gaagaacgcc gtctggccta cgttggcgta acccgcgcga tgcagaaact gacgctgacc
    17101 tacgcggaaa cccgccgtct gtatggcaaa gaagtttacc atcgcccgtc gcgctttatc
    17161 ggcgagctgc cggaagagtg tgtggaagag gtgcgcctgc gcgccacggt aagccgcccg
    17221 gtcagccatc aacggatggg tacgccgatg gtcgagaacg acagcggcta caagctcggc
    17281 cagcgcgtac gccacgctaa gtttggtgaa ggcaccattg tcaatatgga aggcagcggt
    17341 gagcatagcc gtttgcaggt ggcgttccag ggccagggta ttaaatggct ggtggcggct
    17401 tatgcccggt tggagacggt gtgacgttgc gggatgcggt gcgccacacc gcattccgca
    17461 tcagcacctt acttagcgca cttcttcaca tcaatattcc agtcagcacc agacgcaacc
    17521 tgatgtttct gcgcaaaatt atccatattc agcgccacgg aaacattcaa taagctgttc
    17581 agatacacca tcggctggcc ttccggcaca tcgccgaagc ttgcgacata cggcattttc
    17641 cctgcgtatt tttgctggga accttcggaa atggtcacac acaacgtgtc gttgagtttg
    17701 attcctgcct gattcagcaa ctcgtcgctg atattgctcc agacgttgcc gtactgaata
    17761 tcgagaatcg ggatattgcc cttcacttca ccttttgttg cttttgcttt ctgataagaa
    17821 agttcgacaa cttttgcagg aagctctggc cccacctgtt cgaacgtaat cgctccggaa
    17881 gccagacgcg caccagtgta ggcatataca tcgcgaccat ggaaggtata tgacttttca
    17941 gaacctttca ggcggttagc tttttcgtcg atttcccgga cgctatcaat acccagagtt
    18001 tgtgccacca aagtgagcgt accgttatct ggtgaaacga agtattgacc gtttttggtt
    18061 ttcagcacta ccgatttacg gtcagtgcct acgcccggat cgaccacgct aacaaacacc
    18121 gaaccttgcg gccagtaact ggcagtctga tacaggcgat aagcgccttc ccagatgtta
    18181 tacggtggaa tttcgtgtgt taaatcaaag attttaagat tatggtcaac gccgaatgcg
    18241 acccctttca tcgccgagac ggcaccatct ttcaggctaa aatcagtttg taaaatcaaa
    18301 gcgttatccg cataggcgga tgatgatccc agacacgtta ataaaaataa cgttgcgagc
    18361 gtttttttca tttttgtttc ccttcattta tctgatgatt ttccagctgt gtagccagtc
    18421 ccagtttgtc tggcctcctc gagtgtacaa tgcttaacct ttccgctcga cggaaatcat
    18481 ggtgacaaaa ggatagcgtt gccacgggat tgccccgcct ttcatataca tatgtgaaat
    18541 tgtgccatca agataaagca actgctcaac gttcagtttc gctttggcat aacaggcaaa
    18601 atcataaaaa tttgtcgcct gctggctcaa caaaaacacg gcgttccctt gtttattaat
    18661 ccctacaccg ttacgaattt tacgcgaggc aacattcggg tgaatacgcg gattaattac
    18721 gccgttttcc atcaacattg gccctgactg caccgcaaac tgaatctctt tactggtttt
    18781 aaaggcatcc agacgaacga tgccgacttt atctcccgcg acataaaaca cgccgccagg
    18841 acgaataaag aaattccctt cacctgaagc gagatttaac gccaccttct gctgaccgtt
    18901 ttcgatgtac aaaccgagcg gcgcatagct ttcatcatag atgccgccgt tcatcgccat
    18961 ctgcacctga ccctgactat taatatccgc cagcagagca tgtaacgttc cccacgcttc
    19021 gccattggct ttttgccagt acattttcac ccgctctgtt tgaggattaa cgatatacgc
    19081 ctgtacggtc agcgccggat ctgagagtgc gcaatcatca gcggcaacag caaacaaggg
    19141 aagtaaggtg agggtgagaa aaatccgttt gagattcaag gtgatcattc ctttaccaat
    19201 gagtagccga tgcgccatta taggtcctgg atgtgggatt tttttatcct gttagcgacc
    19261 ttgacgagta ccaaaaagcg cgaagttcaa ctattgttct gtggtgttct gttgcgtgtt
    19321 gacggcaaaa ttttgctggc gtaacatgcg cgcacgatca ctctaagagg acattcgcct
    19381 tggacacacc cagtagatac tggctcacta tcctgtcatc caggatcaac tcctaaggct
    19441 atcccttttt gctgatagcc ttagcggttg tcagcgacct caatttttcc cgtcgcgctg
    19501 agtcaggctg tttaatggtc tgaaacccaa tttgtttctg tgtgcccacc gaactgtccg
    19561 atattttaag cattgggagt cccggtcatg ctgagcgcat ttcaactgga aaataaccga
    19621 ctgacccggc tggaagtcga agagtcacaa ccccttgtaa atgcagtatg gattgatctt
    19681 gtcgaaccgg acgacgacga gcgactgcgc gtacaatctg aacttggcca gagcctggca
    19741 acccgcccgg aactggaaga catcgaagca tcggcacgtt tctttgaaga cgatgacggc
    19801 ctgcatattc actccttctt cttctttgaa gatgcggaag atcacgccgg taactccact
    19861 gtggcattta ccatccgtga tggtcgtctg tttactctgc gtgagcgtga actgcccgct
    19921 tttcgtctat atcgtatgcg tgcccgtagc cagtcgatgg tagacggtaa tgcctacgag
    19981 ctgctgctgg atctgttcga aaccaaaatc gaacagttgg cagatgaaat tgaaaatatc
    20041 tacagcgacc tggagcagtt gagccgggta attatggaag ggcatcaggg cgatgagtac
    20101 gacgaggcgc tctccacgct ggcggaactg gaagatatcg gctggaaagt tcgcctgtgt
    20161 ctgatggata cccagcgtgc gctcaacttc ctggtgcgta aggcgcgttt accgggcggg
    20221 caactagagc aggcgcgtga aatcctgcga gatatcgaat ccctgctgcc gcataacgaa
    20281 tctctgttcc agaaggtgaa cttcctgatg caggcggcga tgggctttat caacatcgag
    20341 cagaaccgca tcatcaaaat cttctcggtg gtatccgtcg tgttcctgcc gccaacactg
    20401 gttgcttcca gctacgggat gaactttgag tttatgccag aactgaagtg gagtttcggc
    20461 tacccaggcg cgattatctt tatgatcctc gcgggcctgg caccgtatct gtactttaag
    20521 cggaagaact ggttgtaata tgagtgccgg ataaagctat tttttatccg gcttatttct
    20581 aaaaattgtc ttttttaaag tcatgcggat tgaaaactcg ctctatttta ttttcttgtg
    20641 ttaattgata ctcagtaaaa tcgttaatac caatatgagt gttacgataa gggtattcat
    20701 taataaccat taagtaattt ttcacgatgc aattatcatt gctcttaatt gtcgggaaac
    20761 agtagagatc ttcatacggc ccttcgatat attttttata gtcagtgaag ccattaccga
    20821 tatcaatgac ataccaggta tgccaactac cttctggtag gggatgttga tttttaattt
    20881 cattctgatg cttcagatac caatttattt tatcgctgtc agtccatggc agatgatcaa
    20941 cgaccagaaa gactgaattg ccagcatcag agtatgtata caaaatctgt acaggacgca
    21001 atgtccataa attatagatg cagcatagaa gaaataaaca taatataatt ttctggagtt
    21061 tacttttcat agataatttt cgattctttc gatagtgtta aaattggtga aaaaaggttt
    21121 gaaagcaaag tctttatgcc gttgtaaaaa gaaccatatg cgaaaaaatc tatatttgct
    21181 gtaagtcttg ttttttatat cagttacatc caaaccaaag tggtcttgtg ctatgaaatg
    21241 aatggtagcg ctccagccca taggatattt ttgaaaattt aaaagagaga ttttttgagc
    21301 tgagatatca tgaatgctga ttcccaggcc attaaaatta tctttaaaat cattgaattt
    21361 atttagtctt gatgaaagta attcgatctt aatcttttgt aggagaagag gccggtatcc
    21421 tatcgtaggt gttgaagaaa tatcattttc gatacattct ttgattacac gtattgggct
    21481 attataatca tatttgttta ttcgttcatg aaaagataag ttcaattcct ggctatggaa
    21541 actgttgcca tttccatatc taaaatgatc aatcagatcc tggatgagtg ttttgtattg
    21601 tccaaaaaag gaaaacattt ttgccagatc ttttatttct gcaaataaaa tatcaataca
    21661 ttcactttgt gatatttttg ttccggaagt tggaacacta aaagcaccat aagtagaatt
    21721 cggcccagga aaatggtatt taatgaggcg gtaaggatca acttttgcag agatgtcgct
    21781 taagccgagt gagagaaaat cttgttcttc catatcccca tattgcatat catcagcaga
    21841 aaaatcatcg aataaagtaa tagtttcgaa aagtgtacag ggcatattaa ttgatgagcc
    21901 taatatgttg ttcatttata ttctcctttc ttataattaa gggcaatgtt aatttgataa
    21961 gaaataatag ggtaaaaaaa ttttaaaaaa gaaatgcact tcacattttt cattacattc
    22021 tgaagagaac ttggttaatg attggtcttg ctcatcgcta ataacataat gctgaattta
    22081 ctaaaaatga aatttattaa agcaaatgaa aatatacaac gacgtctgga aattctgcct
    22141 ttccttatgt gagtctcctg tcagcatggc aatatgccca ctctcatgca agagtcggca
    22201 tattttttca gaatatattt attttttatt tggacgttct acgctgcgta taaatcgcat
    22261 ccatcacaaa aattgccagc gccacccaaa taaaggcgaa ggtcaccatc ttatcggcac
    22321 ccggtttttc accataaaac gtcacagcca gcaggaacat cagcgtcggg ccaatgtact
    22381 gaaaaaagcc taacgttgag agacgcaggc gtgtcgcagc cgcagtaaaa cacaacagcg
    22441 gtacggtagt gacaatcccg gcggcgatca gcagtaaatt cagtgacatc gggttttgcc
    22501 ccatatggct agttgagctg tcggcaatag caaacaggta aatcgccgcc acgggcagca
    22561 accacatagt ttcgattaac atgccggttt gcgcttcaac agcaatcttc ttgcgtacca
    22621 ggccgtagaa ggcaaaacta aacgccagcc ccagcgcgat aatcggtagt gaaccaaaag
    22681 tccacaactg aaccaacacg ccacatatcg ccagaatcac cgccagccat tgcatccggc
    22741 ggaatcgctc gccgaggaaa atcatcccta gcacaatgtt caccagcggg ttaataaagt
    22801 aaccaaggct cgcttccagc atatggtgat tgttcaccgc ccagataaac agtagccagt
    22861 tgccaccaat cagcacggca gagactgcca gcataaaaat tttctgtggc gtctgaatca
    22921 gcgtttttaa ataggaccac tggcggcaga tgctcatcag caccaccata aagaaaaacg
    22981 accagatcac gcgatgcgtc aggatctcat cggcgggcac gtagtaaatt agcttgaagt
    23041 acgctggcgc aataccccaa ataaaataag cggcaagagc gagtaatacg ccctgccgcg
    23101 tttgttttgc atccatcggg aatactcatt tttaattggt aacagcagtt tacctgcttt
    23161 tacgttatca acctaccata taggtggctg tagcactggc aatataaagc tgttcttcat
    23221 tgtgtaattc aacgcgggcg acggcgactt tattgcctgc acgtaacagg ctactggtag
    23281 cagtaaaacg ctcgcctctg cctgggcgca gataatcgac gcgaagatca atggtcccca
    23341 ttcgcgatag ccgctgacgt agttcatctt cactgatggt ttcgtggcgg gttaaggtac
    23401 ttcccacgca caccagaccg gcggcgacat ccagcgccga cgcaatgacc ccgccgtgca
    23461 aaatgctttg cgcccagtta cccaccatca ttggctgatt tttaaaggcc agctgtgcga
    23521 actctttttc gtaacgctcc agttccatcc ccaatgcgcg gttaaatggc atgtgataaa
    23581 caaacatctc acccactaat ttcagggctt gttcagcggt cagtacggca gacatatcat
    23641 ccttacactt cattagttaa tgaaatgttg attttatgct tctttgttgt tggtttctac
    23701 tttaggaagg gataactaac ggctatggag ttaagtatgt aaaatagccc gcagaaaaat
    23761 attcacctta tcaataattc gttacggaga acacgagcga tgcggactct gcagggctgg
    23821 ttgttgccgg tgtttatgtt gcctatggca gtatatgcac aagaggcaac ggtgaaagag
    23881 gtgcatgacg cgccagcggt gcgtggcagt attatcgcca atatgctgca ggagcatgac
    23941 aatccgttca ccctttatcc ttatgacacc aactacatca tctataccca aaccagcgat
    24001 ctgaataaag aagcgattgc cagttacgac tgggcggaaa atgcgcgtaa ggatgaagta
    24061 aagtttcagt tgagcctggc gtttccgctg tggcgtggga ttttaggccc gaactcggtg
    24121 ttgggtgcgt cttatacgca aaaatcctgg tggcaactgt ccaatagcga agagtcttca
    24181 ccgtttcgtg aaaccaacta cgaaccgcaa ttgttcctcg gttttgctac cgattaccgt
    24241 tttgcaggtt ggacgctgcg cgatgtggag atggggtata accacgactc taacgggcgt
    24301 tcagacccga cctcccgtag ctggaaccgc ctttataccc gcctgatggc agaaaacggt
    24361 aactggctgg tagaggtgaa gccgtggtat gtggtgggta atactgacga taacccggat
    24421 atcaccaaat atatgggtta ctaccagctt aaaattggct atcacctcgg tgatgcggtg
    24481 ttgagtgcga aaggacagta caactggaac acgggctacg gcggcgcgga gttaggctta
    24541 agttaccaga tcaccaaaca tgtgcgcctt tatactcagg tttacagcgg ttacggcgaa
    24601 tcgctcatcg actataactt caaccagacc cgcgttggtg taggggttat gctaaacgac
    24661 ttgttttgat gaacggttga gtggttggca aatctggaat ccagtatcca ggattaccct
    24721 ctcagagact aaaagcattg cagtttctcg cgcaggcgct gaaaatagcg cctgttttta
    24781 tttcaggcaa tcggggtgaa tgtggcgcag gcggaagtgt tgaatctgga gtccggagct
    24841 aaacaggttt tacaagaaac ctttggctac caacagtttc gccccggcca ggaagaaatt
    24901 atcgacactg tgctttccgg ccgcgattgc ctcgtcgtca tgcccactgg tggcggaaaa
    24961 tccctttgct atcaaattcc tgccttattg ctaaacggcc ttaccgtggt tgtttcaccg
    25021 ctgatttcgt tgatgaaaga tcaggtggat caactgcaag ccaacggcgt ggcggcggcg
    25081 tgccttaact cgacgcaaac ccgcgagcag caacttgaag ttatgacagg ctgtcgcacc
    25141 gggcaaatcc gcctgcttta tatcgcgccg gaacgtctga tgctggataa ctttcttgag
    25201 catctggcgc actggaatcc ggtgttatta gccgttgatg aagcgcactg tatctcccaa
    25261 tggggtcacg atttccgccc ggaatacgct gccctcggtc agctgcgtca gcgttttccg
    25321 acgctgccgt ttatggcgct gactgccaca gccgatgaca ccacgcgcca ggatatcgtg
    25381 cgcctgctgg ggctgaacga tccgctgatt caaatcagca gttttgaccg tccgaatatt
    25441 cgctacatgc tgatggagaa attcaaaccg ctcgatcagt tgatgcgtta tgtacaggaa
    25501 caacgcggta agtcggggat tatttactgt aatagtcgtg cgaaagtaga agacaccgct
    25561 gcgcgcctgc aaagcaaggg aattagcgcg gcggcctatc atgccgggct ggaaaataat
    25621 gttcgcgctg atgtgcagga gaaattccag cgcgatgacc tgcaaattgt ggtggcgacg
    25681 gtggcgttcg gcatgggcat caataaacca aacgttcgct tcgtggtgca ttttgatatt
    25741 ccgcgcaaca ttgaatccta ttatcaggaa accggacgcg ccgggcgtga tggcctgccc
    25801 gcggaagcga tgctgtttta cgatccggcg gatatggcgt ggctgcgtcg ttgtctggaa
    25861 gagaaaccgc aggggcagtt acaggatatc gaacgtcata aactcaatgc gatgggcgcg
    25921 tttgccgaag cgcaaacttg ccgtcgtctg gtgctgctga actactttgg cgaagggcgg
    25981 caggagccgt gcgggaactg cgatatctgc ctcgatccgc cgaaacaata cgatggttca
    26041 accgatgctc agattgccct ttccaccatt ggtcgtgtga atcagcggtt tgggatgggt
    26101 tatgtggtgg aagtgattcg tggcgctaat aaccagcgta tccgcgacta tggtcatgac
    26161 aaactgaaag tctatggcat gggccgtgat aaaagccatg aacattgggt gagcgtgatc
    26221 cgccagctga ttcacctcgg cttggtgacg caaaatattg cccagcattc tgccctacaa
    26281 ctgacagagg ctgcgcgtcc ggtgctgcgc ggcgaatcct ctttgcaact tgccgtgcca
    26341 cgtatcgtgg cgctcaaacc gaaagcgatg cagaaatcct tcggcggcaa ctacgatcgc
    26401 aaactgttcg ccaaattacg caaactgcgt aaatcgattg ccgatgaaag caatgtcccg
    26461 ccgtacgtgg tgtttaacga cgcaaccttg attgagatgg cggaacagat gccgatcact
    26521 gccagcgaaa tgctcagcgt taacggcgtt gggatgcgca agctggaacg ctttggcaaa
    26581 ccgtttatgg cgctgattcg tgcacatgtt gatggcgatg acgaagagta gtcagcagga
    26641 taaaaaagtg ccaggatgaa gactccgtaa acgtttcccc cgcgagtcta atgtatgttg
    26701 atgttattcc tcaccgtcgc catggtgcac attgtggcgc ttatgagccc cggtcccgat
    26761 ttcttttttg tctctcagac cgctgtcagt cgttcccgta aagaagcgat gatgggcgtg
    26821 ctgggcatta cctgcggcgt aatggtttgg gctgggattg cgttgcttgg cctgcatttt
    26881 attatcgaaa aaatggcctg gctgcataca ctgattatgg tgggcggtgg cctgtatctc
    26941 tgctggatgg gttatcagat gctgcgtagc gcactgaaaa aagaggtcgt ttctgcgcct
    27001 gcgccacagg tagaactggc aaaaagcgga cgcagtttcc tgaaaggctt actgaccaat
    27061 ctcgccaatc cgaaagcgat tatctacttt ggttcggtat tctcgctgtt tgtcggcgat
    27121 aacgtcggca ccactgagcg ctggggcatt ttcgcgttga ttattatcga aacactggcg
    27181 tggtttaccg tcgttgccag cctgtttgcc ctgccgcaaa tgcgccgtgg ttatcaacgt
    27241 ctggcgaagt ggattgatgg ttttgccggg gcgttatttg ccggatttgg cattcatttg
    27301 attatttcgc gttgatgcca gacgcgtcat cagagtaagt cggataaggc gtttacgccg
    27361 catccgacat tatttttcac gcatgcctcg ccgatgctaa cagcgctccc accagcataa
    27421 acaacgagcc gaaaatctta ttcagcgcct tcatctgctt tggtccttta atccatagag
    27481 caatccgttg agcaagggtg gcgtaaccga tcatcacaat aatatcgacc acaatagtcg
    27541 tgacgccgag cacgatatac tgcatcagct gcggctgttg cggcatgatg aattgcggaa
    27601 atagcgccgc cagaaacaca atacttttgg gattggtgag attcacaaaa actgcgcgct
    27661 ggaacaaatg tcgacgcgat tgagtagagg ccagcgattt aagatcaatt gcgccagcgg
    27721 cgcgccactg ctgaatccca agccaaatca gataagctgc gcccgcccac ttcaacactt
    27781 caaacgcaat cactgagcgg gaaaatagcg tccccaaacc cacgccaacc agcacaatat
    27841 gaatcgccag tccggtctga agcccagcaa tagacgccac cgcgccgcga taaccatggt
    27901 tgagcgaggt ggtcatggtg ttgattgcgc cagagcctgg cgacaggctt aaaatgatcg
    27961 atgtcagcag gtaggcaaac caccattcta aggtcatgat gaactcccgg tgtgtctatt
    28021 tttgtgccac aatacgctac tgtcgcagcg ttgtgtcagg cacgcttaaa aaaacgattt
    28081 tacgtggttt aagaggcaga ttacccgatg tttcagcagc aaaaagactg ggaaacaaga
    28141 gaaaacgcgt ttgctgcttt taccatggga ccgctgactg atttctggcg tcagcgagat
    28201 gaagcagagt ttactggtgt ggatgacatt ccggtgcgct ttgtccgttt tcgcgcacag
    28261 caccatgacc gggtggtagt catctgcccg gggcgtattg agagctacgt aaaatatgcg
    28321 gaactggcct acgacctgtt ccatttgggg tttgatgtct taatcatcga ccatcgcggg
    28381 cagggacgtt ccggtcgcct gttagccgat ccgcatctcg ggcatgttaa tcgctttaat
    28441 gattatgttg atgatctggc ggcattctgg caacaggaag ttcagcccgg tccgtggcgt
    28501 aaacgttata tactggcaca ttcgatgggc ggtgcgatct ccacattatt tctgcaacac
    28561 catccaggtg tatgtgacgc cattgcgcta actgcgccaa tgtttgggat cgtgattcgt
    28621 atgccgtcat ttatggcacg gcagatcctc aactgggccg aagcgcatcc acgtttccgt
    28681 gatggctatg caataggcac cgggcgctgg cgcgcgttgc cgtttgctat caacgtactg
    28741 acccacagca gacagcgata tcgacgtaac ttacgctttt atgctgatga cccaacgatt
    28801 cgcgtcggtg ggccgaccta ccattgggta cgcgaaagta ttctggctgg cgaacaggtg
    28861 ttagccggtg cgggtgatga caccacgcca acgcttctct tacaagctga agaggaacgc
    28921 gtggtggata accgcatgca tgaccgtttt tgtgaactcc gcaccgctgc gggccatcct
    28981 gtcgaaggag gacggccgtt ggtaattaaa ggtgcgtacc atgagatcct ttttgaaaag
    29041 gacgcaatgc gctcagtcgc gctccacgcc atcgttgatt ttttcaacag gcataactca
    29101 cccagcggaa accgctctac agaggtttaa atttcttatg taccaggttg ttgcgtctga
    29161 tttagatggc acgttacttt ctcccgacca tacgttatcc ccttacgcca aagaaactct
    29221 gaagctgctc accgcgcgcg gcatcaactt tgtgtttgcg accggtcgtc accacgttga
    29281 tgtggggcaa attcgcgata atctggagat taagtcttac atgattacct ccaatggcgc
    29341 gcgcgttcac gatctggatg gcaatctgat ttttgctcat aacctggatc gcgacattgc
    29401 cagcgatctg tttggcgtag tcaacgacaa tccggacatc attactaacg tttatcgcga
    29461 cgacgaatgg tttatgaatc gccatcgccc agaagagatg cgctttttta aagaagcggt
    29521 gttcaaatat gcgctgtatg agcctggatt actggagccg gaaggcgtca gcaaagtgtt
    29581 cttcacctgc gattcccatg aacaactgct gccgctggag caggcgatta acgctcgctg
    29641 gggcgatcgc gtcaacgtca gtttctctac cttaacctgt ctggaagtga tggcgggcgg
    29701 cgtgtcgaaa ggccatgcgt tggaagcagt ggcgaagaaa ctgggctaca gcctgaagga
    29761 ttgtattgcc tttggtgacg ggatgaacga cgccgaaatg ctgtcgatgg cggggaaagg
    29821 ctgcattatg ggcagtgcgc accagcgtct gaaagatctg catccggagc tggaagtgat
    29881 tggtactaat gccgacgacg cggtgccgca ttatctgcgt aagctctatt tatcgtaatc
    29941 gttctttatt tggccagttg tcaacctgat acttcgctac aatggatact cgttaatcaa
    30001 agagttttcc attgtggcgc tacttatcat caccacgatt ctgtgggcct tctcctttag
    30061 cttttatggc gagtaccttg cggggcacgt cgatagctat tttgcggtgc tggtgcgcgt
    30121 tggcctggcg gcactcgttt ttttgccgtt tctgcgtacc cgtggcaata gcctgaaaac
    30181 ggtcggcctg tatatgctgg tgggcgcgat gcagctcggc gtgatgtata tgctgagttt
    30241 tcgcgcctat ctctacctga cggtctccga attgttgctg ttcaccgtac tgacgccgct
    30301 ctacattacg ttgatttatg acatcatgag taagcgccgt ctgcgctggg gctatgcttt
    30361 tagcgccttg ttggcggtga ttggtgccgg gattattcgc tatgatcagg tcaccgacca
    30421 cttctggatt ggcctgctgc tggtgcagct ctccaatatc acttttgcca tcggcatggt
    30481 gggttacaaa cgtctgatgg aaactcgccc gatgccgcag cataacgcct ttgcgtggtt
    30541 ctatcttggc gcgttcctgg tggcggtgat tgcatggttc ttgctgggaa atgcgcagaa
    30601 aatgccacaa accacgttgc aatggggcat tctggtgttt cttggcgtgg tggcttccgg
    30661 gattggctac tttatgtgga actacggcgc aacccaagta gacgccggaa cgctgggtat
    30721 tatgaataat atgcacgttc cggcagggct gctggtaaac ctggctatct ggcaccaaca
    30781 gccgcactgg ccaacgttca ttacaggcgc gctggtgatc ctggcctcac tgtgggtgca
    30841 tcgtaagtgg gtcgctccgc gctcttcaca aacggcagat gatcgcaggc gtgattgcgc
    30901 gctgagcgaa taaacgcttc agtaatcggc tgacgctgct cgccatcgcg cacggcggcg
    30961 tacagtcggc tccacaagcc ttcgcccagg gttttggtca ccaccagacc ctggcgctca
    31021 aaactctcta ctacccaatg cggcagggcg gcaataccca tccgcgcggc aaccatctga
    31081 atcaacaata aggtgttatc gacgcttttc agtgacgggc tgacgcctgc cggctgaaga
    31141 aaatgccgcc agacatccag tcggctacgc tgcaccggat aaatcaacag cgtctcgctg
    31201 gcgagatctt ccggtgtaat acgcgttttt gccgccagcg gatggtcagg tgccaacacc
    31261 agacgcactt cataatcgaa catcggcgaa taatgcaggc cactgcgcgg cagaatatct
    31321 gacgtcatca ccagatccag ctcgccctgt tgcaacgcag gctgcgggtc aaacgtcacg
    31381 cccgatttaa agtccatctc gacctgcggc cagttcttat ggaaattttc taacgcgggc
    31441 gtcagccact gaatacagct atggcactca atggcgatgc gcagacgcgt ctgctgcggt
    31501 tcattgcagg cttgcagtgc ctggctaatt tgtggcagca cctggtttgc cagttgcaac
    31561 aggatttctc cctgcggtgt aaagcgaagc ggctggctct tacgcacaaa tagccggaag
    31621 ccaaggcgtt gttccagatc gctaaactgg tgagacaggg cggattgcgt ctgatgcaac
    31681 gtcgccgcag cggctgcgag cgagccgcag ttccgcaacg cttgtagcgt tttcagatgt
    31741 tttacttcga tcatgaaagt ccttcacttc ggcatgaata atttgcgctt gaggaatata
    31801 cagtaaccgc caattatgga tgtgtaaaca tctggacggc taaatactaa aaattccata
    31861 aggggcatat aatgacaatt cttaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
    31921 gcgagctgaa aaaagcgcaa gagagttatt gggcggggaa ctccacgcgt gaagaactgc
    31981 tggcggtagg gcgtgaattg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
    32041 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtctgctgc
    32101 tgggtaacgt tccggcgcgt catcagaaca aagatggttc ggtagatatc gacaccctgt
    32161 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
    32221 aatggtttaa taccaactat cactacatgg tgccggaatt cgttaaaggc caacagttca
    32281 aactgacctg gacgcagctg ctggacgaag tggacgaggc gctggcgctg ggccacaagg
    32341 tgaaacctgt gctgctgggg ccggttacct acctgtggct gggtaaagtg aaaggtgaac
    32401 agtttgatcg cctgagcctg ctgaacgaca ttcttccgat ttatcagcaa gtgctggcag
    32461 aactggcaaa acgcggcatt gagtgggtac agattgatga acccgcgctg gtactggaac
    32521 tgccgcaggc gtggctggac acatacaaac ccgcttacga cgcgctccag ggacaggtga
    32581 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
    32641 tgcctgttca gggtctgcat gtcgatcttg tacatggtaa agatgacgtt gctgaactgc
    32701 acaagcgtct gccttctgac tggctgctgt ctgcgggtct gatcaatggt cgtaacgtct
    32761 ggcgcgccga tcttaccgag aagtatgcgc aaattaagga cattgtcggc aaacgcgatt
    32821 tgtgggtggc atcttcctgc tcactgctgc acagccccat cgacctgagc gtggaaacgc
    32881 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct gcaaaaatgt catgaactgg
    32941 cactgctgcg cgatgcgctg aacagtggtg acacggctgc tctggcagag tggagcgctc
    33001 cgattcaggc gcgtcgtcac tctactcgtg ttcataatcc ggcagtagaa aagcgtctgg
    33061 cggcgatcac cgcccaggac agtcagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
    33121 agcgtgcgcg ttttaaactg cccgcgtggc cgaccaccac gattggttcc ttcccgcaaa
    33181 ccacggagat tcgtaccctg cgtctggatt ttaaaaaggg taatctcgac gccaataact
    33241 accgcacggg cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac
    33301 tggatgtgct ggtacatggc gaggctgagc gtaatgacat ggtggaatac tttggcgagc
    33361 acctcgacgg ttttgtcttt acgcaaaacg gttgggtaca gagctacggt tcacgctgcg
    33421 tgaagccacc gattgttatt ggtgacgtta gccgcccggc accgattacc gttgagtggg
    33481 cgaagtatgc gcaatccctg actgataaac cggtgaaagg gatgttgacc ggcccggtga
    33541 ctattctctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaaa
    33601 ttgcgctggc gctgcgtgat gaagtggcgg acctggaagc cgctggaatt ggcatcatcc
    33661 agattgatga accggcactg cgcgaaggtt taccgctgcg tcgcggcgac tgggatgcct
    33721 atctccagtg gggcgtggag gctttccgta tcaacgccgc cgtggcgaaa gatgacacac
    33781 aaatccacac tcacatgtgt tactgcgagt tcaacgacat catggattcg attgcggcgc
    33841 tggacgcaga cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt
    33901 ttgaagagtt tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa
    33961 acgtaccgag cgtagaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
    34021 cagagcgtct gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
    34081 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt gcgtcgggga tgacgaatat
    34141 taacgagtca ctgtaaattg cagtacgata atggcttcca gcactggaag ccattttgca
    34201 tgagtgccag attgaagctg atcttcagga gcagaaataa ccgaatcgtt tttttcgcaa
    34261 aacacagttt tgagtgattc cgttcagtaa tcaacgctac gttatttatt ctcacgttgc
    34321 acgagattgt atcccttttc actaacgtgg tagtgaggca tagacttatt cccgctatcc
    34381 tcaataataa ccaaaccttc gttagctaat ttgttaacaa tgttaacaac attcccgaat
    34441 cctggaattt gccttatgga tagtgtgcga tcgagattca tccagttcca gtttatatct
    34501 ttctcagtca taacgttcag cacgttaagt tcatttttag tgatcacgat attctgtacc
    34561 aaaattgatg agtatcttat tgaaagatca caatgtaaat catgctcctg taatggctgg
    34621 atcagaagag gttgtcttgt tgttctttga taagattcaa tccatattga gaaactctgt
    34681 agcgtggtct agagttgccc tcaccattta cgatatcaac caacccatga ttaaccagct
    34741 ttgttacgat attcgctacg ttgccaaatc ctggaatatt tcgtatagct aaagtacgat
    34801 ccagcaccat ccagctccag tcaatatctt tctcagccat aactttcagc acgttaaatt
    34861 caactatcgt aatcgtcatt ttgttgcatc cggatgaaat aagtcagtat ctttaatttt
    34921 aaagtcttct agagttgcag tatgtacatt attccataat tcatatccat ctaaaccttt
    34981 cggttcacct gtttcccatc ctaaattaca ataacgctga tactcgcgac attcatgagt
    35041 atagtagttc agatcgtatt tttcaggaag caattcttta tttgctattt tctttagcct
    35101 tgaaatcatt acatcattag ctggatcatg atcaaatctg gagatatgct tttcaacaat
    35161 agcgatacct tccctgttga ttgttacgcc atctatcgtc aggttttcaa ttggaccacc
    35221 ggcaaggtcg ggattaaatg gtcttcctga tactgctcct ctggctccga aatgctcaag
    35281 tgattccaaa tcagggaatc tcattcttcc cagaacaact ccagcaacta ttgaagcacc
    35341 agcgatgagt aaccctttgt tatccataac ttgatcgtaa ccagcaggtg catctcctcc
    35401 caacttgttt gctgtttccc tgaatccttc gatattccca ttgtatatgc cgccagcagc
    35461 aagcagtctg ccaaccgctt tactattgag tgttttgagg ccattatcat ctttagtttt
    35521 tggccgtggt ataaacagac ggttgtagtt atcgggaaat gccaaataac taaattcagt
    35581 attagcaatc agatgcctgg agagtatata ttcataactg acagtgatgg tttctgtatc
    35641 cagattgttc tcagtaaaca ggtgattgat tgctgataag agcgcgccgc gaagttgaat
    35701 gttgtaatat ttctcccatc gaccaaatct attgatccga tagaagtcaa actccatgaa
    35761 caactgctcg ttattgttaa tagcgttaat aagtaacggg gagcttttgt caatcaattt
    35821 acagaaacta aggccatgca attgtgaccc tttgcctgta ttcgtaatac tattagagag
    35881 cgagaacgcg aagatttcat cttcatggcc aatctgccag cgattaccta ttgatgcgct
    35941 cgttccacaa cgagaggaaa tacatccttg ttgttctccg gttatgttca ggtaaataat
    36001 atcactcatc cttgaaaaac cttttgttaa ttcttttgtg aaagaaaaat agtattgtta
    36061 ttttttacct ttatttacgc ctaaaaggga tgaactaaaa tgtgaaaaac aaaatgtttc
    36121 actttaatat ctttttatca ggaaggtctg agataattta taattaatgt gaatgtgttg
    36181 ataataatta atgtttgttt gagaatggga gttgatggta aatctctaag gtgtctgcca
    36241 gtgaagagac tcacttatga ctattacttg ccgactttct taaggtcaaa tagcggagtc
    36301 gtgctctcca atcaattcat actgattatt ttttggggga cagagatggc agcaaaacgc
    36361 actcgttcac tcaccatctc aaatatagac taagaggaaa ctaattccgc ttcccaccat
    36421 actgcttaaa ccactccagc atacgctgcc agccatcttt cgcagattcg gcatgatagc
    36481 tcgggcgata atcggcgttg aatgcatgcc cggcatccgg gtagacgata atctctgctt
    36541 tcgcattagc agcccgcagc gcctggcgca tggtttcaac gctctcctgc ggaatgctgt
    36601 tatcctgacc accatataag ccgagaaccg gcgcgttaag atcggttgcg atatcaacag
    36661 gttgtttcgg tgaattcagc gacttatcgc ctgtcagttt gccgtaccat gccaccgccg
    36721 ctttcagttg cgggttatgc gcagcataca gccaggtgat acgtccaccc cagcagaatc
    36781 cggtgatcat taaacgatga acatcgccgc cgttgcggga agcccaactg gcgacatgat
    36841 cgagatcggc cagcacctgc gagtcaggca cttttgctac cagaccgcta agcaacgtgg
    36901 ggatatcggc aaaatcattc ggatcgcctt cgcggaagta aagttcaggt gcaatagcca
    36961 gatacccctc cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc acgccaaaaa
    37021 tatcctgcac tacaatgacc actggcagtg ggccatcgct ctgctttggt ctggcatggt
    37081 aagcaggcat gttatcccct tgtgaaggga tagaggtgaa gcccgccaca atcgcgtcgt
    37141 ccggagtctg aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat ccagattgtt
    37201 gtgttgttgc catggtattc tccgtaccct tataaaaatg ttgcgcaatg ttaactatag
    37261 tcagcatgca acaaatcaca ttgcctgaat cggctcatct tttatgcagt cctgcagaat
    37321 gaagagtgat ttatgtgatt tgcatcactt ttggggggta aatttatgca acgcatttgc
    37381 gtcatggtga tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt ctttttgctt
    37441 cttctgacta aaccgattca cagaggagtt gtatatgtcc aagtctgatg tttttcatct
    37501 cggcctcact aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg gcgacccgga
    37561 tcgtgtggaa aagatcgccg cgctgatgga taagccggtt aagctggcat ctcaccgcga
    37621 attcaccacc tggcgtgcag agctggatgg taaacctgtt atcgtctgct ctaccggtat
    37681 cggcggcccg tctacctcta ttgctgttga agagctggca cagctgggca ttcgcacctt
    37741 cctgcgtatc ggtacaacgg gcgctattca gccgcatatt aatgtgggtg atgtcctggt
    37801 taccacggcg tctgttcgtc tggatggcgc gagcctgcac ttcgcaccgc tggaattccc
    37861 ggctgtcgct gatttcgaat gtacgactgc gctggttgag gctgcgaaat ccatcggcgc
    37921 gacgactcac gttggcgtga cggcttcttc tgataccttc tacccaggtc aggaacgtta
    37981 cgatacttac tctggtcgcg tagttcgtca ctttaaaggt tctatggaag agtggcaggc
    38041 gatgggcgta atgaactatg aaatggaatc tgcaaccctg ctgaccatgt gtgcaagtca
    38101 gggcctacgt gccggtatgg tagcgggtgt tatcgttaac cgcacccagc aagagatccc
    38161 gaatgctgag acgatgaaac aaactgaaag ccaagcggtg aaaatcgtgg tggaagcggc
    38221 gcgtcgtctg ctgtaatcct cttctcctgt ctgaaggccg acgcgttcgg ccttttgtat
    38281 ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca gcacaattct
    38341 attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat catggtttac
    38401 gcagttattg cgttggtggg tgtggcaatt ggctggctgt ttgccagtta tcaacatgcg
    38461 cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt aagcgcggca
    38521 aaacaacaaa ttacccaaag cgagcactgg cgcgctgagt gcgagttact caataacgaa
    38581 gtgcgcagcc tgcaaagtat taacacctct ctggaggccg atctgcgtga agtaaccacg
    38641 cggatggaag ccgcacagca acatgctgac gataaaattc gccagatgat taacagcgag
    38701 cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta tttttgagca cagcaatcgc
    38761 cgggttgatg agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct gcgtgaacaa
    38821 ctggatggtt ttcgccgaca ggtacaggac agctttggta aagaagcaca agaacgccat
    38881 accctgaccc acgaaattcg taatctacaa caactcaacg cgcaaatggc ccaggaagcg
    38941 atcaacctga cccgcgcgct gaaaggcgat aacaaaaccc aggggaactg gggcgaagtg
    39001 gtactgacgc gggtgctgga ggcttccgga ctgcgtgaag ggtatgaata cgaaacccag
    39061 gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt gcggctaccg
    39121 caggggaaag atgtggtgat cgatgccaaa atgacgctgg tcgcctatga acgctacttc
    39181 aacgcggaag acgactacac acgcgaaagc gcattgcagg agcatattgc ttcggtacgt
    39241 aatcacatcc gcttgcttgg gcgcaaagac tatcaacagc tgccggggct acgaacgctg
    39301 gattacgtgc tgatgtttat ccctgtggaa cccgcttttt tactggcgct tgatcgccag
    39361 ccggagctga tcaccgaagc attaaagaac aacataatgc tggttagccc caccacgctg
    39421 ctggtggcgt tgcgtactat cgccaacctt tggcgttatg agcatcaaag ccgtaacgcc
    39481 cagcaaatcg ccgatcgcgc cagcaagctg tacgacaaga tgcgtttgtt cgtcgatgac
    39541 atgtcagcga ttggtcaaag tctcgacaaa gcgcaggata actatcgcca ggcaatgaaa
    39601 aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg cggtttaggg
    39661 gtagaaatta aacgcgagat taatccggat ttggctgaac aagcggtgag ccaggatgaa
    39721 gagtatcgac tgcggtcggt tccggagcag ccgaatgatg aagcttatca acgcgatgat
    39781 gaatataatc agcagtcgcg ctagcccatt gggggtagtt aagccgggta gaaatctagg
    39841 gcatcgacgc ccaatctgtt acacttctgg aacaattttt tgatgagcag gcattgagat
    39901 ggtggataag tcacaagaaa cgacgcactt tggttttcag accgtcgcga aggaacaaaa
    39961 agcggatatg gtcgcccacg ttttccattc cgtggcatca aaatacgatg tcatgaatga
    40021 tttgatgtca tttggtattc atcgtttgtg gaagcgattc acgattgatt gcagcggcgt
    40081 acgccgtggg cagaccgtgc tggatctggc tggtggcacc ggcgacctga cagcgaaatt
    40141 ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg aatccatgct
    40201 caaaatgggc cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg agtatgttca
    40261 ggcgaacgct gaggcgctgc cgttcccgga taacaccttt gattgcatca ctatttcgtt
    40321 tggtctgcgt aacgtcaccg acaaagataa agcactgcgt tcaatgtatc gcgtgctgaa
    40381 acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc cgctgagcaa
    40441 agcctatgat gcatactcct tccatgtgct gccgcgtatt ggctcactgg tcgcgaacga
    40501 cgccgacagc tatcgttatc tggcagaatc catccgtatg catcccgatc aggatactct
    40561 gaaagccatg atgcaggatg ccggattcga aagtgtcgac tactacaatc tgacggcagg
    40621 ggttgtggcg ttgcatcgtg gttataagtt ctgacaggag accggaaatg ccttttaaac
    40681 ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc tcacccgcgc
    40741 tgaaaacggc ccgctcgcgt ctgttgggca aagtgctgcg cgtggaggta aaaggctttt
    40801 cgacgtcatt gattctggtg ttcagcgaac gccaggttga tgtactgggc gaatgggcag
    40861 gcgatgctga ctgcaccgtt atcgcttacg ccagtgtgtt gccgaaactt cgcgatcgcc
    40921 agcagcttgc cgccctgatt cgcagtggtg agctggaagt gcagggcgat attcaggtgg
    40981 tgcaaaactt cgttgcgctg gcagatctgg cagagttcga ccctgcggaa ctgctggccc
    41041 cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga ggcgcaaagt
    41101 tcctgcatca cggcattaag cgccagcaac gttatgtggc agaagccatt actgaagagt
    41161 ggcgtatggc acccggtccg cttgaagtgg cctggtttgc ggaagagacg gctgctgtcg
    41221 agcgtgctgt tgatgccctg accaaacggc tggaaaaact ggaggctaaa tgacgccagg
    41281 tgaagtacgg cgcctatatt tcatcattcg aactttttta agctacggac ttgatgaact
    41341 gatccccaaa atgcgtatca ccctgccgct acggctatgg cgatactcat tattctggat
    41401 gccaaatcgg cataaagaca aacctttagg tgagcgacta cgactggccc tgcaagaact
    41461 ggggccggta tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc tttttccgcc
    41521 gcatattgcc gatcagctgg cgttattgca ggacaaagtt gctccgtttg atggcaagct
    41581 ggcgaagcag cagattgaag ctgcaatggg cggcttgccg gtagaagcgt ggtttgacga
    41641 ttttgaaatc aagccgctgg cttctgcttc tatcgcccag gttcataccg cgcgattgaa
    41701 atcgaatggt aaagaggtgg tgattaaagt catccgcccg gatattttgc cggtcattaa
    41761 agcagacctg aaacttatct accgtctggc tcgctgggtg ccgcgtttgc tgccggatgg
    41821 tcgccgtcta cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga ttgatgaact
    41881 gaatttgctg cgggaatctg ccaacgccat tcagcttcgg cgaaattttg aagacagccc
    41941 gatgctctac atcccggagg tttaccctga ctattgtagt gaagggatga tggtgatgga
    42001 gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg gcaccaacat
    42061 gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact caggtttttc gcgacagctt
    42121 tttccatgcc gatatgcacc ctggcaacat cttcgtaagc tatgaacacc cggaaaaccc
    42181 gaaatatatc ggcattgatt gcgggattgt tggctcgcta aacaaagaag ataaacgcta
    42241 tctggcagaa aactttatcg ccttctttaa tcgcgactat cgcaaagtgg cagagctaca
    42301 cgtcgattct ggctgggtgc caccagatac caacgttgaa gagttcgaat ttgctattcg
    42361 tacggtctgt gaacctatct ttgagaaacc gctggccgaa atttcgtttg gacatgtact
    42421 gttaaatctg tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc aactggtgtt
    42481 actccagaaa accctgctct acgtcgaagg ggtaggacgc cagctttatc cgcaactcga
    42541 tttatggaaa acggcgaagc ctttcctgga gtcgtggatt aaagatcagg tcggtattcc
    42601 tgcgctggtg agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa tgccagaact
    42661 gcctgaactg gtttacgaca gtttgcgcca gggcaagtat ttacagcaca gtgttgataa
    42721 gattgcccgc gagcttcagt caaatcatgt acgtcaggga caatcgcgtt attttctcgg
    42781 aattggcgct acgttagtat taagtggcac attcttgttg gtcagccgac ctgaatgggg
    42841 gttgatgccc ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg gttggcgcaa
    42901 aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc ggccttgttt
    42961 aatcatcatc taccacagag gaacatgtat gggtggtatc agtatttggc agttattgat
    43021 tattgccgtc atcgttgtac tgctttttgg caccaaaaag ctcggctcca tcggttccga
    43081 tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac caaagcagga
    43141 taaaaccagt caggatgctg attttactgc gaaaactatc gccgataagc aggcggatac
    43201 gaatcaggaa caggctaaaa cagaagacgc gaagcgccac gataaagagc aggtgtaatc
    43261 cgtgtttgat atcggtttta gcgaactgct attggtattc atcatcggcc tcgtcgttct
    43321 ggggccgcaa cgactgcctg tggcggtaaa aacggtcgcg ggctggattc gcgcgttgcg
    43381 ttcactggcg acaacggtgc agaacgaact gacccaggag ttaaaactcc aggagtttca
    43441 ggacagtctg aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg aactgaaagc
    43501 gtcgatggat gaactacgcc aggccgcgga gtcgatgaag cgttcctacg ttgcaaacga
    43561 tcctgaaaag gcgagcgatg aagcgcacac catccacaac ccggtggtga aagacaatga
    43621 aactgcgcat gaaggcgtaa cgcctgctgc tgcacaaacg caggccagtt cgccggaaca
    43681 gaagccagaa accacgccag agccggtggt aaaacctgct gcggacgctg aaccgaaaac
    43741 cgctgcacct tccccttcgt cgagtgataa accgtaaaca tgtctgtaga agatactcaa
    43801 ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat tatcgcggtg
    43861 atcgtgatat tcctgtgtct ggtctatttc gccaatgaca tctatcacct ggtatccgcg
    43921 ccattgatca agcagttgcc gcaaggttca acgatgatcg ccaccgacgt ggcctcaccg
    43981 ttctttacgc cgatcaagct gacctttatg gtgtcgctga ttctgtcagc gccggtgatt
    44041 ctctatcagg tgtgggcgtt tatcgcccca gcgctgtata agcatgaacg tcgcctggtg
    44101 gtgccgctgc tggtttccag ctctctgttg ttttatatcg gcatggcgtt cgcctacttt
    44161 gtggtctttc cgctggcatt tggcttcctt gccaataccg cgccggaagg ggtacaggta
    44221 tccaccgaca tcgccagcta tttaagcttc gttatggcgc tgtttatggc gtttggtgtc
    44281 tcctttgaag tgccggtggc aatagtgctg ctgtgctgga tggggattac ctcgccagaa
    44341 gacttacgca aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg gatgctgctg
    44401 acaccgccgg atgtcttctc gcaaacgctg ttggcgatcc ctatgtactg tctgtttgaa
    44461 atcggtgtct tcttctcacg cttttacgtt ggtaaagggc gaaatcggga agaggaaaac
    44521 gacgctgaag cagaaagcga aaaaactgaa gaataaattc aaccgcccga cagggcggtt
    44581 gtcatatgga gtacaggatg tttgatatcg gcgttaattt gaccagttcg caatttgcga
    44641 aagaccgtga tgatattgta gcgcgcgctt ttgacgcggg agttaatggg ctactcatca
    44701 ccggcactaa cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag tattcatcct
    44761 gttggtcaac ggcgggcgta catcctcacg acagcagcca gtggcaagct gcgactgaag
    44821 aagcgattat tgagctggcc gcgcagccag aagtggtggc gattggtgaa tgtggtctcg
    44881 actttaaccg caacttttcg acgccggaag agcaggaacg cgcttttgtt gctcagctac
    44941 gcattgccgc agaattaaac atgccggtat ttatgcactg tcgcgatgcc cacgagcggt
    45001 ttatgacatt gctggagccg tggctggata aactgcctgg tgcggttctt cattgcttta
    45061 ccggcacacg cgaagagatg caggcgtgcg tggcgcatgg aatttatatc ggcatcactg
    45121 gttgggtttg cgatgaacga cgcggactgg agctgcggga acttttgccg ttgattccgg
    45181 cggaaaaatt gctgatcgaa actgatgcgc cgtatctgct ccctcgcgat ctcacgccaa
    45241 agccatcatc ccggcgcaac gagccagccc atctgcccca tattttgcaa cgtattgcgc
    45301 actggcgtgg agaagatgcc gcatggctgg ctgccaccac ggatgccaat gttaagacac
    45361 tgtttgggat tgcgttttag agtttgcgga actcggtgtt cttcacactg tgcttaatct
    45421 ctttattgat aagattaagt aacagcatgg agcgagcctc gccgtcgggt tcggtgaaaa
    45481 tggcctgaaa accttcgaac gcgccttcgg taataatcac cttatcaccc ggataagggg
    45541 ttgccggatc gacaatgtct ttcggtttat ataccgatag ctgatgaatc accgccgatg
    45601 ggactatcgc tggcgatgcg ccaaagcgca cgaagtggct gacaccgcgg gtcgcgttga
    45661 tagtcgtggt atgaatcact tctggatcaa attccacaaa caggtagttg gggaacaatg
    45721 gttcactgac tgcagtacgt ttgccacgca ccattttttc cagggtgatc atcggtgcca
    45781 ggcaattcac agcctgtctt tcgaggtgtt cctgggcacg ttgaagttgc ccgcgcttgc
    45841 agtacagtaa ataccaggat tgcataatga ctcttatccg tttaatcggg gcgcaaggat
    45901 agcaaaagct ttacgctaag ttaattatat tccccggttt gcgttatacc gtcagagttc
    45961 acgctaattt aacaaattta cagcatcgca aagatgaacg ccgtataatg ggcgcagatt
    46021 aagaggctac aatggacgcc atgaaatata acgatttacg cgacttcttg acgctgcttg
    46081 aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg gaaatcactg
    46141 aaattgctga ccgtactttg cgtgccggtg ggcctgcgct gttgttcgaa aaccctaaag
    46201 gctactcaat gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg gcgatgggca
    46261 tggggcagga agatgtttcg gcgctgcgtg aagttggtaa attattggcg tttctgaaag
    46321 agccggagcc gccaaaaggt ttccgcgacc tgtttgataa actgccgcag tttaagcaag
    46381 tattgaacat gccgacaaag cgactgcgtg gtgcgccgtg tcagcaaaaa attatctccg
    46441 gcgatgacgt cgatcttcac cgtatcccca ttatgacctg ctggccggaa gacgccgcgc
    46501 cgctgattac ctgggggttg accgtgacgc gcggcccgca taaagagcgg cagaatctgg
    46561 gcatttatcg ccagcagctg attggtaaaa acaaattgat tatgcgctgg ctgtcgcatc
    46621 gcggcggcgc gctggatttc caggagtggt gtgcggcgca tccgggcgaa cgtttcccgg
    46681 tttctgtggc gctgggtgct gatcccgcca caattctcgg tgcagtcacc cccgttccag
    46741 atacgctttc agagtatgcg tttgccggat tgctgcgcgg caccaagacc gaagtggtga
    46801 agtgtatctc caatgacctt gaagtgcccg ccagtgcgga gatagtgctg gaagggtata
    46861 tcgaacaagg cgaaactgcg ccggaagggc cgtatggcga ccacaccggt tactataacg
    46921 aagtcgatag tttcccggta tttactgtga cgcatattac ccagcgtgaa gatgcgattt
    46981 atcattccac ctataccggg cgtccgccgg atgaacctgc ggtgctgggt gtcgcactga
    47041 acgaagtgtt tgtgccgatt ctgcaaaaac agttcccgga aattgtcgat ttttacctgc
    47101 cgccggaagg ctgctcttat cgcctggcgg tagtgacgat caaaaaacag tacgccggac
    47161 acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg tacactaaat
    47221 ttgtgatcgt ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg atttgggcga
    47281 ttaccacccg tatggacccg gcgcgggaca cggtgttggt cgaaaatacg cctattgatt
    47341 atctggattt tgcctcgcct gtgtccgggc tgggttcaaa aatggggctg gatgccacga
    47401 ataaatggcc aggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa gatccggatg
    47461 ttgtcgcgca tattgacgcc atctgggatg aactggctat ttttaacaac ggtaaaagcg
    47521 cctgatgcgc gtttgttttg ccctatttat cgatccgaca gagaaagcgc atgacaacct
    47581 taagctgtaa agtgacctcg gtagaagcta tcacggatac cgtatatcgt gtccgcatcg
    47641 tgccagacgc ggccttttct tttcgtgctg gtcagtattt gatggtagtg atggatgagc
    47701 gtgacaaacg tccgttctca atggcttcga cgccggatga aaaaggtttc atcgagctgc
    47761 atattggcgc ttctgaaatc aacctttacg cgaaagcagt catggaccgc attcttaaag
    47821 atcatcaaat cgtggtcgat attccgcacg gtgaggcatg gctgcgcgat gatgaagaac
    47881 gtccaatgat tctcatcgcg ggcggcaccg ggttctctta tgcgcgctcg attttgctga
    47941 cggcgctggc gcgtaaccca aaccgtgata tcaccattta ctggggcggg cgtgaagagc
    48001 agcatcttta tgatctctcc gagcttgagg cgctttcgct taagcaccct ggtctgcaag
    48061 tggtgccggt ggttgaacaa ccggaagcgg gctggcgtgg gcgtactggc actgtgttaa
    48121 cggcggtatt gcaggatcac ggtacgctgg cagagcatga tatctatatt gccggacgtt
    48181 ttgagatggc gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg cgggaagatc
    48241 gcctgtttgg cgatgcgttt gcatttatct gagatataaa aaacccgccc ctgacaggcg
    48301 ggaagaacgg caactaaaac tttatacagt ggcatttaga tctatgtcgc atccggcata
    48361 cgctgcctga tgcgacgctg gcgcgtctta tcaggcctac attggtgctg gatcggtaga
    48421 ccggataagg cgtcacgccg catccggcaa gtggttaaat ccgctcaaac accgtcgcaa
    48481 taccctgacc cagaccgata cacatcgtcg ccagaccaaa ctgggcatct ttgcgctcca
    48541 tcagattcag cagcgtggtg ctgatacgcg caccggaaca acccagcgga tgacccagcg
    48601 cgatcgcgcc accgttgagg ttgatcttct cgtcaatctg ctcaatcagt cccagatctt
    48661 taatacatgg caggatctgc gcggcaaagg cttcgttcat ctcaaacacg ccgatatcac
    48721 tggcagaaag ccccgctttt ttcagcgcca gcttcgaggc cggaaccggg ccgtaaccca
    48781 taatcgatgg gtcacaacca acgaccgcca tcgaacgcac acgggcgcgc ggcttaagac
    48841 ctaattcacg ggcgcggctt tcactcatca ccaacatggc agctgcacca tcggaaagcg
    48901 cagaagatgt gcccgccgtt accgtaccgc tgacgggatc aaacgccgga cgcagcgtgg
    48961 cgagggcttc cacggtggtt tccgggcgaa tcacttcgtc gtaattaaac tgcttcagga
    49021 cgccgtcggc atcgtgacca ccggtcggga taatttcatt tttaaatgca cccgactgcg
    49081 tggcagccca ggcgcgggcg tgtgaccgcg cggcaaaggc atcctgcatt tcacggttga
    49141 taccgtgcat acgcgccagc atttctgccg ttaagcccat catgcccgcc gctttggcga
    49201 cattgcggct caggccgggg tgaaaatcga cgccgtgact catcggcaca tggcccatat
    49261 gctccacgcc gccaaccaga catgcctgcg catcgccggt catgatcatt cgtgctgcat
    49321 catgcagtgc ctgcatggat gaaccacaca agcgattgac ggtaaccgcc gggacagagt
    49381 gtggtacttc tgccagcagc gccgcgttac gggcgatatt aaaaccctgc tccagcgtct
    49441 gctgcacaca accccagtaa atatcgtcga gggccgccgc ttccagcgcc gggttacgcg
    49501 ccagcaggct acgcattaaa tgagcggaga gatcttctgc acgcacgtga cgaaaagcac
    49561 cgcccttcga acggcccatc ggggtgcgaa ttgcatcgac aatgacaacc tgttccattg
    49621 tgactcctta agccgttttc aggtcgccaa ccggacgggc tggctcaacc gggggatagt
    49681 acggttcgtt atgacgcgct ttattacgca gaccttccgg cacttcatac agcgggccga
    49741 ggtgctgata ttgctgtgcc atatcgaggt attttgcgct accgagggtg tccagccagc
    49801 ggaacgcacc gccgtggaac ggagggaagc ccaggccgta gaccagcgcc atatccgctt
    49861 ctgccggagt ggcgataatg ccttcttcca gacagcggac cacttcgttg accatcggga
    49921 tcatcatgcg ggcgataatc tcttcttcgc tgaaatcgcg cttcggctgg ctcacttctg
    49981 ccagcaggtc gtcaacgacg gcgtcttctt ctttcttcgg cttacctttg ctgtcttctt
    50041 tataacgcca gaaaccgagg ccgttcttct gaccaaagcg gttggcatca aacagcgcgt
    50101 cgatggtatc gcgataatct ttctgcatcc gctgcgggaa gcctgctgcc atgacagcct
    50161 gggcatgatg cgcggtgtca atgcccacaa cgtccagcag atatgccgga cccataggcc
    50221 agccaaactg tttttccatc actttgtcga tcttgcggaa atccgcgccg tcgcgcagca
    50281 gctggctgaa acccgcgaag taggggaaca gcacgcggtt aacgaagaat ccggggcagt
    50341 cgttaaccac aatcggcgtt ttgcccatct tgctcgccca ggcgacgact ttcgcgatgg
    50401 tttcgtcgga gcttttctcg ccgcgaataa tttccaccaa cggcatccgg tgcaccgggt
    50461 taaagaagtg catcccgcag aagttttccg gacgttccag cgcgttggcc agttcgctga
    50521 taggaatggt tgaagtgtta gacgccagca cggtatccgg gcgtactttc tgctcggttt
    50581 ctgccagtac ggcttttttc actttcgggt tttcgacaac cgcttctacc acaacatcca
    50641 cgcggtcaaa accagagtaa tcgagcgttg ggtggattgt ggagatcacg ccagccagtt
    50701 tcagaccatc gatcttgccg cgctcaagct gcttgttcag cagtttcgcg gcttcggtca
    50761 tgccgagggt taacgacttg tcgttgatat ctttcatgac aaccggtacg cctttccacg
    50821 cagactggta agcgatgccg ccacccataa tgcctgcgcc cagcacagcg gcctgtttcg
    50881 gggtttcaac gtctttggtg agtttcttcg ctttgccttt tacatattga tcgttaagga
    50941 aaatgccgac cagtgcgcgg gcttcgttgg tgtgcgccag cgggacaaag cttttgtttt
    51001 ccagatttaa tgcttcttca cgaccaaaac gggccgccgc ttcaatggtt tttactgcgg
    51061 tgatgggggc cggataatgt ttccccgccg tttgtgcgac catcccttta gcgatggtga
    51121 agctcatggt ggcttcaatc ttgctcagtt ttagcggttc cagcttcggc tgacgtttcg
    51181 ctttccagtc gaggtcgccg ttaatggcct ggcgtaaaat cgccatcgcc ccttcaacca
    51241 gtttttctgc tttgactaca ccatccacca gaccgatttt cagcgcctga tccgcgccga
    51301 catctttacc ggcggcaatg atttccagcg cactgtcagc gcccagcata cgtggcatac
    51361 gtacagaacc gccaaagccc ggcatgatgc ccagtttggt ttccggcaga ccgatgcgca
    51421 gatccggcgt cgccagacga taatcggtcg ccagcacgca ttcgcagcca ccgcccagcg
    51481 catagccgtt gacggcagca atggtcggca ccgggagatc ttccaggcga ttaaacacgc
    51541 tattggcaaa gtgcagccac tgacttaact gttcttcagg aacgaggaac agggacaaaa
    51601 attcggtgat atcagcaccg acgataaagg ctgatttgtt cgaacgtagc agcagccctt
    51661 ttagatctga ttgctgttcc agcacgccga tggcctcgcc gaggctggcg acggtcgcag
    51721 tgtcgagttt attaactgaa cctggggcat caaataccag ttcggcaatg ccatcttcca
    51781 gccagtcaag gtacagggtg tcgcctttgt aaagcatgtc agtctcctga atccgcaagg
    51841 tgatctggtc gtaccagatg catcgaagtg tgtattttgt gttaaaaata tgcaaataaa
    51901 agattaaaga aatgccgatc tgatcacgct cggcagaaat cacgctctgg atgaacgatg
    51961 tgctaagatg cggagactta aggtcaaaaa aacagaaggg taaaaaatgg aatcactggc
    52021 ctcactctat aaaaatcata tagctacctt acaagaacgg actcgcgatg cgctggcgcg
    52081 cttcaagctg gatgcgttac ttattcactc cggcgaactg ttcaacgttt ttctcgacga
    52141 tcatccctat ccgtttaaag tgaatccgca attcaaagcg tgggtgccgg taactcaggt
    52201 gccaaactgc tggctgttgg tggatggcgt gaacaagccg aaactgtggt tttatctgcc
    52261 ggttgattac tggcacaacg tcgaaccgct gccgacctcc ttctggactg aagatgtaga
    52321 agtgatcgcg ctgccgaaag ccgatggcat tggtagcctg ctgcctgctg cgcgcgggaa
    52381 tatcggttat atcggtccgg tgccggagcg tgcgctgcaa ctgggtattg aggccagcaa
    52441 tatcaacccg aaaggggtga tcgactacct gcattactac cgctccttca aaaccgagta
    52501 cgaactggcc tgtatgcgtg aagcgcagaa aatggcggtc aacggtcatc gcgcggcaga
    52561 agaagcgttc cgttctggca tgagcgagtt cgatatcaat attgcctatc tgaccgcgac
    52621 cggtcatcgt gataccgacg taccttacag caacattgtg gcgcttaacg aacacgctgc
    52681 ggtgttgcat tacaccaaac tggaccacca ggcaccggaa gagatgcgca gcttcctgct
    52741 ggatgccggg gccgaatata acggctatgc ggctgacctg acccgtacct ggtcggcaaa
    52801 aagcgataac gactacgcac agctggtgaa agacgtaaat gatgaacaac tggcgctgat
    52861 cgccaccatg aaagcaggcg tcagctatgt ggattaccac atccagttcc atcagcgcat
    52921 cgccaaactg ctgcgtaaac atcaaatcat caccgatatg agtgaagaag cgatggtcga
    52981 aaacgatctc accggaccgt ttatgccgca cggtatcggc catccgctgg gcctgcaggt
    53041 gcatgacgta gccggtttta tgcaggatga tagcggtacg cacctcgcgg caccggcaaa
    53101 atatccgtac ctgcgctgca cccgtattct ccagccgggc atggtgttaa ccatcgagcc
    53161 gggtatctac ttcatcgaat cgctactggc accgtggcgt gaagggcggt tcagtaaaca
    53221 ctttaactgg cagaaaattg aagcactgaa accgttcggc ggcattcgta tcgaagacaa
    53281 cgtggtgatc cacgaaaaca acgtggaaaa catgacccgg gatctgaaac tggcgtgatg
    53341 gaaagctggt taattcctgc ggcaccagtc acggtcgttg aagagatcaa aaagagccgc
    53401 ttcataacga tgttggcgca taccgatggc gttgaggcgg cgaaagcgtt tgttgaatcg
    53461 gtgcgggcag aacaccccga tgcccgccac cattgcgtgg cgtgggtggc gggtgcgccg
    53521 gatgattctc aacagctcgg tttctctgac gacggggaac cggcgggaac ggccggtaaa
    53581 ccgatgctcg cccaactaat gggcagcggc gtcggggaaa ttaccgctgt ggtggtgcgc
    53641 tactacggcg gcatattgct tggtaccggt gggttagtga aagcgtatgg cggcggagtg
    53701 aatcaggcgc tgcgccagct aacgacccaa cgcaagacgc cattaaccga atatactttg
    53761 caatgtgaat atagtcagtt aaccggcatt gaagcgttgc tggggcagtg tgacggcaaa
    53821 attatcaaca gtgattatca ggcattcgtt ctgctgcggg tggcgcttcc ggcggcgaaa
    53881 gtggctgaat tttccgcaaa gctggcggat tttagccgtg gttcattgca attgttagcg
    53941 attgaagaat aatccccact tcgttttgca gaactaagga agcggcagag atgcattttc
    54001 gcgccattac ccgaatcgtt ggactactgg tcatcttatt ttcagggacc atgattatcc
    54061 ctgggctggt agcactcatc taccgggatg gagcgggccg cgcttttacc cagacctttt
    54121 ttgtcgccct cgccattggc tctatgctgt ggtggccgaa ccgcaaagag aagggcgaac
    54181 tgaaatcccg tgaggggttt ctgatagtgg tgctgttctg gaccgtgctg ggtagcgtcg
    54241 gtgcgctccc ttttatcttc tcggaaagcc cgaacctcac gattaccgat gcgttttttg
    54301 aatctttctc tggcctgacc accacggggg ccactacgct ggtggggctg gattcgctcc
    54361 ctcacgccat cctcttttat cgccagatgc tgcaatggtt tggcgggatg gggatcatcg
    54421 tgttggcggt tgcgatactg cctatcctcg gcgtgggtgg gatgcagctc tatcgcgcag
    54481 aaatgcccgg cccgctgaaa gataacaaaa tgcgcccgcg aattgcggaa acggcgaaaa
    54541 ccctgtggtt gatttatgtc ttgctgaccg tcgcctgtgc gctggcgttg tggtttgccg
    54601 gaatggatgc ctttgatgcc atcggccata gctttgcgac tatcgctatt ggcggcttct
    54661 cgacacatga tgccagtatc ggttatttcg acagcccgac aattaacact atcattgcta
    54721 tcttcctgtt gatctccggc tgtaactacg gtctgcactt ttcgctgtta agtgggcgta
    54781 gtctgaaggt ttattggcgc gatccggaat ttcgcatgtt tatcggcgta cagtttacgc
    54841 tggtgattat ttgtaccctc gtgctgtggt ttcataatgt ctatagttcg gcgctgatga
    54901 caattaacca ggcgtttttc caggtggtgt cgatggcgac aaccgccggg tttacgactg
    54961 acagcattgc ccgctggccg ctctttttgc cggtactgct cttatgttca gcttttatcg
    55021 gcggttgtgc cgggtcaacg ggcggtggcc tgaaagtgat ccgcatcctg ctgctgttta
    55081 agcaggggaa ccgtgagctg aaacgactgg tgcatccgaa cgccgtctat agcattaagc
    55141 tggggaatcg cgcactgccg gaacgtatcc tcgaagccgt ttggggattt ttctccgcct
    55201 atgcattggt gtttattgtc agtatgctgg cgattatcgc cacgggcgtg gatgactttt
    55261 ctgcctttgc ctcagttgtt gcgacattga ataacctggg gccagggctt ggcgtggttg
    55321 ctgataactt taccagtatg aacccggtgg ctaaatggat cctgattgcc aacatgctgt
    55381 ttggtcgtct tgaggtcttt acattgctgg tgctctttac cccgactttc tggcgtgaat
    55441 gatggagtaa tacgtgaaaa cattaattct tttctcaaca agggacggac aaacgcgcga
    55501 gattgcctcc tacctggctt cggaactgaa agaactgggg atccaggcgg atgtcgccaa
    55561 tgtgcaccgc attgaagaac cacagtggga aaactacgac cgtgtggtca ttggtgcttc
    55621 tattcgctat ggtcactacc actctgcgtt ccaggaattt gtcaaaaagc atgcgacgcg
    55681 gctgaattcg atgccgagcg ccttttactc cgtaaacctg gtggcgcgta aacctgagaa
    55741 gcgtactcca cagaccaaca gctacgcgcg gaagtttctg atgagttcac aatggcgtcc
    55801 cgatcgctgc gcggtcattg ccggggcgct gcgttaccca cgttatcgct ggtatgaccg
    55861 ttttatgatc aagttaatta tgaagatgtc aggcggtgaa acggatacgc gcaaagaagt
    55921 tgtctatacc gattgggagc aggtggcgaa tttcgcccga gaaatcgccc atttaaccga
    55981 caaaccgacg ctgaaataag cataaagaa
//
LOCUS       DAPOZO010000025        52118 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPOZO010000025 DAPOZO010000000
VERSION     DAPOZO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.025"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..795
                     /locus_tag="ROI21_001597"
     CDS             <1..795
                     /locus_tag="ROI21_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDX5110534.1"
                     /translation="EFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHEISE
                     DALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINELDA
                     MTAHVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGAKAT
                     EQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQACHT
                     YAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            805..1572
                     /locus_tag="ROI21_001598"
     CDS             805..1572
                     /locus_tag="ROI21_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110535.1"
                     /translation="MTYSIGIDSGSTTTKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            1569..1826
                     /locus_tag="ROI21_001599"
     CDS             1569..1826
                     /locus_tag="ROI21_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDX5110536.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            1891..2751
                     /locus_tag="ROI21_001600"
     CDS             1891..2751
                     /locus_tag="ROI21_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDX5110537.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQEHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            2819..3997
                     /locus_tag="ROI21_001601"
     CDS             2819..3997
                     /locus_tag="ROI21_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5110538.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTAQLFKLQVVTS
                     "
     gene            complement(4010..4563)
                     /gene="kptA"
                     /locus_tag="ROI21_001602"
                     /pseudo
     CDS             complement(4010..4563)
                     /gene="kptA"
                     /locus_tag="ROI21_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            4813..5496
                     /locus_tag="ROI21_001603"
     CDS             4813..5496
                     /locus_tag="ROI21_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110539.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            5493..5954
                     /locus_tag="ROI21_001604"
     CDS             5493..5954
                     /locus_tag="ROI21_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDX5110540.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            5967..7139
                     /gene="iadA"
                     /locus_tag="ROI21_001605"
     CDS             5967..7139
                     /gene="iadA"
                     /locus_tag="ROI21_001605"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDX5110541.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFTRKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            7204..8115
                     /gene="hypT"
                     /locus_tag="ROI21_001606"
                     /gene_synonym="qseD"
     CDS             7204..8115
                     /gene="hypT"
                     /locus_tag="ROI21_001606"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDX5110542.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(8108..8500)
                     /gene="iraD"
                     /locus_tag="ROI21_001607"
     CDS             complement(8108..8500)
                     /gene="iraD"
                     /locus_tag="ROI21_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDX5110543.1"
                     /translation="MMRQSLQAVLPEISGNKTSSLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(8497..8580)
                     /gene="idlP"
                     /locus_tag="ROI21_001608"
     CDS             complement(8497..8580)
                     /gene="idlP"
                     /locus_tag="ROI21_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDX5110544.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            9173..10003
                     /locus_tag="ROI21_001609"
     CDS             9173..10003
                     /locus_tag="ROI21_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HDX5110545.1"
                     /translation="MSMPLSNALQSQIITDAHFLHHPIVDSEFTRKLKYARMDSENIY
                     LPPLTRGNNHNYDGKSVVEIRKLDISKEPWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHATTELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(10144..10917)
                     /gene="uxuR"
                     /locus_tag="ROI21_001610"
     CDS             complement(10144..10917)
                     /gene="uxuR"
                     /locus_tag="ROI21_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDX5110546.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(11132..12592)
                     /locus_tag="ROI21_001611"
     CDS             complement(11132..12592)
                     /locus_tag="ROI21_001611"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDX5110547.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETIQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTVTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(12673..13857)
                     /gene="uxuA"
                     /locus_tag="ROI21_001612"
     CDS             complement(12673..13857)
                     /gene="uxuA"
                     /locus_tag="ROI21_001612"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDX5110548.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIIEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            14197..15540
                     /gene="gntP"
                     /locus_tag="ROI21_001613"
     CDS             14197..15540
                     /gene="gntP"
                     /locus_tag="ROI21_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDX5110549.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(15783..16685)
                     /gene="fimH"
                     /locus_tag="ROI21_001614"
     CDS             complement(15783..16685)
                     /gene="fimH"
                     /locus_tag="ROI21_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDX5110550.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(16705..17208)
                     /gene="fimG"
                     /locus_tag="ROI21_001615"
     CDS             complement(16705..17208)
                     /gene="fimG"
                     /locus_tag="ROI21_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDX5110551.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(17221..17751)
                     /gene="fimF"
                     /locus_tag="ROI21_001616"
     CDS             complement(17221..17751)
                     /gene="fimF"
                     /locus_tag="ROI21_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDX5110552.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(17761..20397)
                     /gene="fimD"
                     /locus_tag="ROI21_001617"
     CDS             complement(17761..20397)
                     /gene="fimD"
                     /locus_tag="ROI21_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDX5110553.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(20464..21189)
                     /gene="fimC"
                     /locus_tag="ROI21_001618"
     CDS             complement(20464..21189)
                     /gene="fimC"
                     /locus_tag="ROI21_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDX5110554.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(21226..21765)
                     /locus_tag="ROI21_001619"
     CDS             complement(21226..21765)
                     /locus_tag="ROI21_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX5110555.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(21830..22378)
                     /gene="fimA"
                     /locus_tag="ROI21_001620"
     CDS             complement(21830..22378)
                     /gene="fimA"
                     /locus_tag="ROI21_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDX5110556.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAAGFNIQLNDCDTNVASKAAVAFLGTAID
                     VGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
     gene            complement(22859..23455)
                     /gene="fimE"
                     /locus_tag="ROI21_001621"
     CDS             complement(22859..23455)
                     /gene="fimE"
                     /locus_tag="ROI21_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDX5110557.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(23933..24535)
                     /gene="fimB"
                     /locus_tag="ROI21_001622"
     CDS             complement(23933..24535)
                     /gene="fimB"
                     /locus_tag="ROI21_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDX5110558.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            25991..26707
                     /gene="nanC"
                     /locus_tag="ROI21_001623"
     CDS             25991..26707
                     /gene="nanC"
                     /locus_tag="ROI21_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDX5110559.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            26727..27833
                     /gene="nanM"
                     /locus_tag="ROI21_001624"
     CDS             26727..27833
                     /gene="nanM"
                     /locus_tag="ROI21_001624"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDX5110560.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLITVKDNKVTVQN"
     gene            27898..28878
                     /locus_tag="ROI21_001625"
     CDS             27898..28878
                     /locus_tag="ROI21_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HDX5110561.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGACWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            complement(29461..30701)
                     /locus_tag="ROI21_001626"
                     /pseudo
     CDS             complement(29461..30701)
                     /locus_tag="ROI21_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378101.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            30817..31062
                     /locus_tag="ROI21_001627"
                     /pseudo
     CDS             30817..31062
                     /locus_tag="ROI21_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31439..31696
                     /locus_tag="ROI21_001628"
     CDS             31439..31696
                     /locus_tag="ROI21_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110562.1"
                     /translation="MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRAQLDNR"
     gene            31708..32253
                     /locus_tag="ROI21_001629"
     CDS             31708..32253
                     /locus_tag="ROI21_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDX5110563.1"
                     /translation="MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDES
                     ARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
                     QPMTLTGHIRCADPDETGALT"
     gene            32309..33055
                     /locus_tag="ROI21_001630"
     CDS             32309..33055
                     /locus_tag="ROI21_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDX5110564.1"
                     /translation="MDIPRIFTISESEHRIHNPFTEEKYATLGRVLRMKPGTRILDLG
                     SGSGEMLCTWARDHGITGTGIDMSSLFTAQAKRRAEELGVSERVHFIHNDAAGYVANE
                     KCDVAACVGATWIAGGFAGAEELLAQSLKPGGIMLIGEPYWRQLPATEEIAQACGVSS
                     TSDFLTLPGLVGAFDDLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDDFAAE
                     VRAELNIAPKRYVTYARECFGWGVFALIAR"
     gene            33224..33442
                     /locus_tag="ROI21_001631"
                     /pseudo
     CDS             33224..33442
                     /locus_tag="ROI21_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348444.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33480..33596
                     /locus_tag="ROI21_001632"
                     /pseudo
     CDS             33480..33596
                     /locus_tag="ROI21_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227376.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            33841..34962
                     /locus_tag="ROI21_001633"
     CDS             33841..34962
                     /locus_tag="ROI21_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDX5110565.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPSVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTMELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDLAIGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLITP
                     PRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAEV
                     ASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
     gene            34959..35237
                     /locus_tag="ROI21_001634"
     CDS             34959..35237
                     /locus_tag="ROI21_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDX5110566.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTGINDDALKQQIKALLTQ"
     gene            35249..36562
                     /locus_tag="ROI21_001635"
     CDS             35249..36562
                     /locus_tag="ROI21_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDX5110567.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVAMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLLPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGGFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            36575..37381
                     /gene="sgcQ"
                     /locus_tag="ROI21_001636"
     CDS             36575..37381
                     /gene="sgcQ"
                     /locus_tag="ROI21_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HDX5110568.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            37512..37943
                     /locus_tag="ROI21_001637"
     CDS             37512..37943
                     /locus_tag="ROI21_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDX5110569.1"
                     /translation="MINDIKWVQAQRKATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLTAVGNASTDDALFALVSG"
     gene            37955..38587
                     /locus_tag="ROI21_001638"
     CDS             37955..38587
                     /locus_tag="ROI21_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HDX5110570.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMLARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            38604..39386
                     /locus_tag="ROI21_001639"
     CDS             38604..39386
                     /locus_tag="ROI21_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDX5110571.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IVNQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLPHSIK"
     gene            39689..40477
                     /locus_tag="ROI21_001640"
     CDS             39689..40477
                     /locus_tag="ROI21_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDX5110572.1"
                     /translation="MVRKGCNSLVRAEKILTHIAWVGMASYMELLNKFQYPKSSLLNL
                     LNVMVDCGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQELSLKSGLVCHL
                     GAMESISAIYLDKIESPDSVPTSKSWIGKKLELHITALGKALLAWKTREELDYFLEAL
                     TLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVFNMYNRVNYAIS
                     LSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            40482..41387
                     /locus_tag="ROI21_001641"
     CDS             40482..41387
                     /locus_tag="ROI21_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDX5110573.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMALAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            41398..43365
                     /gene="yjhG"
                     /locus_tag="ROI21_001642"
     CDS             41398..43365
                     /gene="yjhG"
                     /locus_tag="ROI21_001642"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylonate dehydratase YjhG"
                     /protein_id="HDX5110574.1"
                     /translation="MSVRNIFADESHDIYTVRTHADGPDGELPLTAEMLINRPSGDLF
                     GMTMNAGMGWSPDELDRDGILLLSTLGGLRGADGKPVALALHQGHYELDIQMKAAAEV
                     IKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMVMRRLIRSLPDAKAVIGVA
                     SCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKVQTIGARFANGELSLQDA
                     RRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPSGEPVWREIARASARA
                     ALNLSQKGITTREILTDKAIENAMTVHAAFGGSTNLLLHIPAIAHQAGCHIPTVDDWI
                     RINKRVPRLVSVLPNGPVYHPTVNAFMAGGVPEVMLHLRSLGLLHEDVMTVTGSTLKE
                     NLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQAKARGLTSTITFPVGNIAPEGSV
                     IKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAGDILVIIGVGPSGTG
                     MEETYQVTSALKHLSYGKHVSLITDARFSGVSTGACIGHVGPEALAGGPIGKLRTGDL
                     IEIKIDCRELHGEVNFLGTRSDEQLPSQEEATAILNARPSHQDLLPDPELPDDTRLWA
                     MLQAVSGGTWTGCIYDVNKIGAALRDFMNKN"
     gene            43472..44821
                     /locus_tag="ROI21_001643"
     CDS             43472..44821
                     /locus_tag="ROI21_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDX5110575.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            45168..46154
                     /locus_tag="ROI21_001644"
     CDS             45168..46154
                     /locus_tag="ROI21_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDX5110576.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRVASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(46195..47199)
                     /locus_tag="ROI21_001645"
     CDS             complement(46195..47199)
                     /locus_tag="ROI21_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HDX5110577.1"
                     /translation="MFIVESYAVAIIMCFITMICWGSWANTTKLVSNKKWEFPLFYWD
                     YSIGLLLCSLLFAFTLGSMGEAGRSFIPDIQQASSSSLMSAILAGIIFNISNILLVAS
                     INLAGMAVAFPVGVGLALALGVITTYIGNPQGDPLILFLGVVCVVIAIIFTAIAYGRV
                     TQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLMTPYSALVLFAVG
                     LFLSNFVLNRLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWCVGLAFSLIASGQ
                     AGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISYIVGIVLIIAANQ
                     "
     gene            complement(47303..47944)
                     /locus_tag="ROI21_001646"
     CDS             complement(47303..47944)
                     /locus_tag="ROI21_001646"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDX5110578.1"
                     /translation="MERIKLAEKIISTCREMNASGLNQGTSGNVSARYTGGMLITPSG
                     IAYSKMTPDMIVFVDDKGKPEAGKIPSSEWLIHLACYKARPELNAVIHTHAVNSTAVA
                     IHNHSIPAIHYMVAVSGTDHIPCIPYYTFGSPELADGVSKGIRESKSLLMQHHGMLAM
                     DVTLEKTLWLAGETETLADLYIKCGGLHHDVPVLSEAEMTIVLEKFKTYGLKA"
     gene            complement(47975..49090)
                     /gene="mtnA"
                     /locus_tag="ROI21_001647"
     CDS             complement(47975..49090)
                     /gene="mtnA"
                     /locus_tag="ROI21_001647"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HDX5110579.1"
                     /translation="MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMAA
                     TAIQDMWVRGAPLIGVVAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDR
                     MIDTLKDLCVSERKDVAWALAAEIAEEDVALCEQIGLHGAEVIREIAQKKPAGSVVNI
                     LTHCNAGWLATVDWGTALSPIYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPH
                     TLIADNAGGHLMQHGDVDLCIVGTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALP
                     SPTIDWTIEDGKSIPIEQRDGKEQSHVYGINPQGELSWVNTAPEGTRCGNYAFDVTPA
                     RYITGFITERGVCAASKSALADMFADLKSKALQGEQH"
     gene            complement(49100..50359)
                     /locus_tag="ROI21_001648"
     CDS             complement(49100..50359)
                     /locus_tag="ROI21_001648"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HDX5110580.1"
                     /translation="MTDSIPSGYKPLTCDTLPGYLSSRLTPSCEPGGLPEEWKVSEVG
                     DGNLNMVFIVEGTHKTIIVKQALPWLRAGGEGWPLSLSRAGFEYNVLCQEAKYAGHTL
                     IPQVYFYDPEMALFAMEYLTPHVILRKELINGKKFPKLAEDIGRFLAQTLFNTSDIGM
                     SAEQKKALTAEFALNHELCKITEDLIFTEPYYNAERNNWTSPELDDAVHKAWADVEMI
                     QVAMRYKYKFMTEAQALLHGDLHSGSIMVTDTDTKVIDPEFGFMGPMAFDIGNYIGNL
                     LLAYFSRPGWDANEQRRADYQEWLLQQIVQTWSVFTREFRQLWDNKTQGDAWSTEMYQ
                     QNRAALEDAQDQFFATLLEDSLVNAGMEMNRRIIGFAGVAELKQIENTELRAGCERRA
                     LTMARDLIVNARQFKNMDSVIQSAKVK"
     gene            complement(51098..51196)
                     /locus_tag="ROI21_001649"
                     /pseudo
     CDS             complement(51098..51196)
                     /locus_tag="ROI21_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
     gene            complement(51286..51654)
                     /locus_tag="ROI21_001650"
     CDS             complement(51286..51654)
                     /locus_tag="ROI21_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDX5110581.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            complement(51817..52038)
                     /locus_tag="ROI21_001651"
     CDS             complement(51817..52038)
                     /locus_tag="ROI21_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDX5110582.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
BASE COUNT        13212 a        12858 c        12608 g        13440 t
ORIGIN      
        1 gagtttaagc ccgttcatgt gatgcaactg ccaaacagcg ttaaggacga tgcctcgcgt
       61 gcattatgga aagccgagat gctgcgcttg caaaaaacgg tagaagaacg ttttgggcac
      121 gagattagcg aagatgctct gcgcgatgcc attgcgctga aaaaccgcga acgtcgcgca
      181 ctggccaact tttatcattt ggggcagtta aatcctccgg cgcttagcgg cagcgacatt
      241 ctgaaagtag tctacggcgc aaccttccgg ttcgataaag aggcgttgat caatgaactg
      301 gatgcaatga ccgcccacgt tcgtcagcag tgggaagaag gccagcgact ggacccgcgc
      361 ccacgtattt taatcaccgg ctgcccaatt ggcggcgcag cagagaaagt ggtgcgcgcg
      421 attgaagaga atggcggctg ggttgtcggt tatgaaaact gcaccggggc gaaagccacc
      481 gagcaatgcg tggcggaaac gggtgatgtg tacgacgcgc tggcggataa atatctggcg
      541 attggctgct cctgtgtttc gccgaacgat cagcgcctgc aaatgctcag ccagatggtt
      601 gaagaatatc aggtcgatgg cgtagttgat gtgattttgc aggcgtgcca tacctacgcg
      661 gtggaatcgc tggcgattaa acgtcatgtg cgccagcagc acaacattcc ttatatcgct
      721 attgaaacag actactccac ctcggatgtc gggcagctca gcacccgtgt cgcggccttt
      781 attgagatgc tgtaaggagt ggcagtgaca tattcgattg gcattgattc cggctcaacc
      841 accaccaaag ggatcttact ggctgacggc gtgattacgc gccgtttcct cgttccaaca
      901 ccctttcgcc cggcaacagc aattactgaa gcctgggaaa ctctgcgcga agggttagag
      961 acaacaccgt ttctgacgct caccggctac ggacggcaac tggtggattt tgccgataaa
     1021 caagtgacgg agatctcctg tcacgggctg ggcgcacggt ttcttgagcc agcaacgcgc
     1081 gcggtaatcg acatcggtgg tcaggacagc aaagtgattc agcttgatga tgacggtaac
     1141 ctgtgcgatt tcctgatgaa tgacaaatgc gcggcgggca ccgggcgttt cctggaggtg
     1201 atctcgcgca cgcttggcac cagcgtcgaa cagctcgaca gcattaccga aaatgtcacg
     1261 ccgcacgcca tcacgagtat gtgcacagtg tttgctgaat cagaagtgat cagcctgcgc
     1321 tctgcgggcg tagcgccaga agcgattctc gccggagtga ttaacgcgat ggcgcggcgg
     1381 agtgccaatt tcattgctcg tctctcctgt gaagcgccga ttctgtttac tggtggcgtt
     1441 agtcattgcc agacgtttgc ccggatgctg gaatctcacc tgggaatgcc ggtaaatact
     1501 caccctgatg cgcaattcgc tggtgcaatt ggcgcggcgg tgatcggtca gcggcagaga
     1561 aaacgcgcat gaaagagtat ctgtttttat ttcactccac ggtcggcgtc atacaaaccc
     1621 gcaaggcgtt gcaggcagcg ggcatgactt ttcgcgtcag cgatattccc cgtgatttac
     1681 gcggcggctg cgggttatgc atttggctga cctgtccgcc tggcgaggaa attcaatggg
     1741 tgatccctgg gcatacggaa tcagtttatt gtcagcagga tggtggctgg cgctgcattg
     1801 cacattacgg gatttctccg cgttaaggat tcattaagat cgctacgtat acttctcgat
     1861 atcttattga tttgccttaa agggattacc gtgacaaaaa gtatttcgtt aagtaaacgc
     1921 atcttcgtta ttgtgattct ttttgtcatt gtggcagtgt gtactttctt cgtgcaatct
     1981 tgcgcccgta aaagcaatca tgcagcatca tttcagaatt accacgccac cattgacggc
     2041 aaagaaattg caggcataac gaataatatc tcttcgctga cctggtcggc acaaagcaat
     2101 actcttttta gcactatcaa caaacctgcc gccattgttg aaatgaccac caatggcgat
     2161 ttgatccgca caattcctct ggattttgtt aaggatctcg agacgatcga atacattggc
     2221 gataatcagt ttgtcattag cgatgagcgc gattatgcca tttatgtgat ttcactgacc
     2281 ccaaactcgg aagtaaaaat cctcaaaaaa ataaaaatcc cgctgcagga atccccaacc
     2341 aattgcggtt ttgaaggatt ggcctattct cgccaggagc acactttctg gttttttaaa
     2401 gagaaaaacc cgattgaagt ctacaaagtt aacgggctgt taagtagcaa tgaattgcat
     2461 atcagcaaag acaaggcact acaacggcaa tttactttag atgatgtgtc cggcgcagaa
     2521 tttaaccaac aaaaaaatac gcttctggtg ctgtctcatg aatctcgcgc actccaggaa
     2581 gtaacgctgg ttggcgaggt tatcggtgaa atgtcactaa ccaaaggcag tcgaggtttg
     2641 tcgcataaca tcaaacaggc cgaaggggtt gcgatggatg cctctggtaa tatttatatc
     2701 gttagcgaac caaatcgttt ttatcgcttt acccctcaat catcgcatta acaggctggc
     2761 atgtttcacc tttttgttat taacttgtta acaacaaaaa tgacaaacaa ggttaaacat
     2821 gccttcgccc tcgcatcccg tagaacgctt ttctttcagc accgcgcttt tcgggatgct
     2881 ggttctgacc ttaggtatgg gtttaggccg cttcctttat acgcctatgt tgcccgtcat
     2941 gatggcggaa ggatcgtttt catttagcca gctctcgtgg attgccagcg gcaactatgc
     3001 ggggtatctg gctggcagtc tgctattttc gtttggcgca tttcaccagc catcgcgcct
     3061 gcgcccattt ctgttggctt ctgccctggc gagcgggttg ttgatcctcg caatggcatg
     3121 gttgctgcca tttattctgg tgttattgat tcgcgtcctg gcgggtgtcg ccagcgccgg
     3181 aatgctgatt tttggttcga cgctgattat gcagcacacg cgccatcctt ttgtgctggc
     3241 agctttgttt tctggcgttg gcattggcat cgcactgggc aatgaatatg ttctggcagg
     3301 cctgcatttt gacctctcct cgcaaacgtt atggcaaggc gccggggcgc tttctggcat
     3361 gatgctgatt gcacttacgc ttttaatgcc ctcgaaaaaa cacgccatca caccaatgcc
     3421 attggcaaaa acggagcaac agataatgag ctggtggtta ctggctattc tgtacggcct
     3481 ggcgggattt ggttatatca tcgtcgcgac ttacctgccg ctcatggcga aagatgcagg
     3541 ctcaccattg ttaaccgccc atctctggac gttagtcggc ttatcaattg tgcctggttg
     3601 ctttggctgg ctatgggcag caaaacgttg gggggctttg ccctgcctga cggcgaattt
     3661 gctggtgcag gctattagtg tgttgcttac tctcgccagc gactcgcctc tcttgcttat
     3721 catcagcagt cttggttttg gcggtacctt tatgggcacg acatctctgg tgatgactat
     3781 cgcccgccaa ctgagcgtac cgggaaatct caatctttta ggctttgtga cactcattta
     3841 cggcattggg caaatcctcg gcccggcgct aaccagtatg cttggcaatg gaacatcggc
     3901 gcttgccagc gccactctct gcggcgcggc ggcgttgttt attgctgcgc ttatcagcac
     3961 agcgcaatta tttaagctgc aagtggttac ttcatgagga gtgtttccct taccactcaa
     4021 gaaactcaac cgcaaccgtt gatgtcagcc agaccccgtt ttccgcttgc caaaaaggga
     4081 tgcctcgttt cgccatttcc tgtgctttga cggttaaaat aactggcgat ccgtgtcttg
     4141 ctcctacttt gcgtgccgtt gcttcatcag cagagagatg cacgtaatgg cgctcccctg
     4201 caatcagccc ttgtttttta tctcgtcaag aaaacggctt gccgtaccgt ggtagagaaa
     4261 ttgtggcggt gttttttcga tgaagccaat ggcgacctgc gaagtcaaat gcccctgaac
     4321 ggcgcgaata cacgtaccat cactggaata actaaagcgc tttttatcgc tggtggcgac
     4381 aacgatatcc agtaatgcgc gggttaaccg cttgcccgct ttttgggcac agagtatgag
     4441 cttatcgata tccgcccatc cttcacggtc cagcacgatg ccaatcgcct ctggtttatg
     4501 tcggaggaca aaacttaaaa acttgctcgt ctctgccagc tctttgtcgt tgtattttgc
     4561 catcgcgttt tcgtccctga attttctgtt tttatttact gatgtcattg tcactgggtt
     4621 tatgaatttt ctgcaactca atcctgcgca gaacctttgc cacacagcgt catttttccg
     4681 ctatgcaaaa aacgcatagc aagaaacata cttgcatttt ccccataccc acatggctga
     4741 taaggttttt cagctccagg aattttcctg gctcaagcaa cacacataca caaaaaaaca
     4801 atggggattg ttatgacaca acaaggggat gctgtagctg gggagctggc gacagaaaaa
     4861 gtgggcatta aagggtatct ggcctttttt ctcaccataa tctttttttc cggtgttttt
     4921 tccggaacag acagctggtg gcgcgttttt gatttttccg ttctgaacgg atcgtttggg
     4981 caactgcctg gtgcgaacgg agccaccacc tcatttcgcg gcgcgggtgg cgcgggggct
     5041 aaagatggct ttctctttgc gctggagctg gcaccgtcag tcattctctc tctggggatt
     5101 atttcaatta ccgatggtct tggtggatta cgcgccgcgc agcagttaat gacacctgtg
     5161 ctaaaaccgt tgttgggcat tccagggatc tgctcgctgg cgctgatcgc taacctgcaa
     5221 aacaccgatg ccgccgccgg tatgaccaaa gagctggcgc aggaaggtga aattaccgaa
     5281 cgcgataaag tgattttcgc cgcatatcaa accagcggta gcgccatcat caccaactac
     5341 ttctcttccg gcgtcgcggt gtttgccttt ctgggcacgt ccgtaatcgt acctttagcc
     5401 gtcatcctgg tgtttaaatt tgtcggagcc aacattttgc gcgtctggct taacttcgaa
     5461 gaacgtcgca acccaacgca aggagcgcaa gcatgacaac tcaggtccgc aaaaacgtca
     5521 tggatatgtt tatcgatggc gcgcgccgtg gctttactat cgccacgacc aacctgctgc
     5581 caaacgtggt gatggcgttt gtcatcattc aggcgctgaa aattaccggc ctgctcgact
     5641 gggtggggca tatctgtgaa cccgtaatgg cgctgtgggg attacccggt gaagcggcaa
     5701 ccgtcctttt agccgcatta atgagtatgg gtggcgcagt cggtgttgcc gccagtctgg
     5761 caaccgctgg cgcattaacc ggtcacgatg tcaccgtttt actgcccgcg atgtatttaa
     5821 tgggcaaccc ggtgcaaaat gtcggtcgct gcctggggac agccgaagtg aatgccaaat
     5881 attacccgca tatcattacg gtctgcgtga ttaacgcatt gctgtcgatc tgggttatgc
     5941 agcttattgt ttaacaagga gttaccatga ttgattatac cgcagccggt tttaccctgc
     6001 tgcagggagc gcatttgtat gcgccggaag atcggggaat ttgcgatgtc ctcgtcgcta
     6061 acggcaaaat tatcgccgtt gccagcaata tcccttctga cattgtaccg aactgcacgg
     6121 ttgtcgatct cagtgggcag atcctctgcc caggttttat tgatcaacac gtccatttga
     6181 ttggcggtgg cggcgaagca ggtcccacga cgcgcacgcc ggaagtggcg ctaagtcgcc
     6241 tgacggaagc gggcgtcacg tcagtggttg gtctgctggg caccgactct atctctcgcc
     6301 acccggaatc cctgctcgcc aagacccgtg cgctcaatga agaaggcatc agcgcctgga
     6361 tgctgaccgg cgcttatcat gtcccttccc gcaccattac gggttccgtg gaaaaagacg
     6421 tggcgattat cgatcgtgtg attggcgtga aatgcgccat ctctgatcac cgttctgccg
     6481 caccggacgt ttatcacctg gccaatatgg cggcagaatc ccgcgttggc ggtttgctcg
     6541 gcggtaaacc tggcgtcacc gtgttccaca tgggcgacag taaaaaggcg ttacagccta
     6601 tttatgacct gctggaaaac tgcgatgtgc cgatcagcaa gctgctgccg acccacgtta
     6661 accgcaacgt accgttgttt gagcaggcgc tggagttcac gcgcaaaggc ggcaccatcg
     6721 atatcaccag cagcattgac gaaccggtcg cccctgccga aggtattgcc cgcgccgttc
     6781 aggcgggtat tccgctggca cgcgtcaccc tcagctccga cggcaacggt agccagccgt
     6841 tcttcgatga cgaagggaat ttaacccata tcggtgttgc cggttttgaa acgttgctgg
     6901 aaaccgtgca ggtgctggtc aaagactatg atttcagtat cagcgatgcc ctgcgcccgc
     6961 tcaccagtag cgtagccggt ttccttaacc tgaccgggaa aggcgaaatt ctgccaggca
     7021 atgatgctga cttgctggtc atgacgccag aactgcgcat tgagcaggta tacgctcgcg
     7081 gcaaactgat ggtcaaagac ggcaaagcct gcgtgaaagg aacgtttgaa acggcttaaa
     7141 cggttgtcag ccgcccgaat ggacgtagaa tgcccgcaag ggcggctgac agagtaaaac
     7201 gtaatggatg actgtggtgc gattttgcat aatattgaaa ccaaatggct ttatgatttt
     7261 ctgaccctgg aaaaatgccg caatttttcc caggcggcag tcagtcgcaa cgtctcgcaa
     7321 ccggcattca gccgccgcat ccgtgcgctg gaacaggcga ttggtgttga attgtttaac
     7381 cgccaggtaa cgccgctgca actctcagaa caagggaaaa tcttccattc gcagatccgc
     7441 catctgttgc aacagttaga gagcaatctg gcagagctgc gtggcggcag cgattacgcg
     7501 caacgaaaaa tcaagatagc cgctgcacac tctctttccc tcgggctgtt accgtccatt
     7561 atcagccaga tgccgccgct ctttacctgg gcaattgaag ctattgatgt cgatgaagcg
     7621 gtcgataaac tgcgtgaagg gcaaagtgac tgtatttttt cctttcacga cgaagatttg
     7681 ctggaagcgc cgtttgacca cattcgctta tttgaatctc aattgttccc tgtctgcgcc
     7741 agtgacgaac acggagaagc actttttaac ctcgcgcagc cacactttcc gttactgaat
     7801 tacagccgca actcctacat ggggcgattg attaatcgca ccctgacgcg ccacagtgag
     7861 ttaagtttca gcaccttttt tgtctcttcg atgagcgagc ttttaaagca ggttgccctc
     7921 gacggctgtg ggattgcctg gctgccggag tacgccatcc aacaagaaat tcgcagcggg
     7981 aagctcgttg tactgaatcg ggacgaactg gtgatcccga ttcaggccta tgcataccgg
     8041 atgaataccc gcatgaatcc cgttgccgaa cgcttctggc gtgaactgcg cgagctggag
     8101 attgtgctta gctgacattc tccagcgtcg cactgcgcca accgccatta tcccagacca
     8161 gatcaaacgt tagcggttca tcgtcgtggt acagcattat ctcaaggctg atcgccagag
     8221 tgtggcagta cgcttctttc aaattaacct gcaacgccgc aatccgtggc tcatgaaata
     8281 acagtgcaga acgcagctga cggcaatacc agctctggag atgtttgtcg ctcgggttat
     8341 gcacttgcca ctcgggcatg cctgaaacaa gtaatgacgg taatgccgaa tgaggagaat
     8401 taaaaagagt gaggagatcg ctgcataccg atttacgcag cgatgacgtt ttattgcctg
     8461 aaatttcagg taaaacagcc tgaagtgatt gtcgcatcat tttgcgcact cctgacgttt
     8521 agcaacatat gcggcttgcc ctgaacaccg ggcaccactg tattgatgct cattttccat
     8581 agccattccc ttttgttcat tacatccatg tcagtctgag acaggcctga atcctgtcag
     8641 caaagtataa aaccttactt gcctatgaat atctactgac ttttcaggaa aaaagtcatg
     8701 atttgataaa aatcatcaaa ctcaaaaaaa aaccgctagt atattctgaa tattccgaat
     8761 agttacgtag atgaaaatac ttgtcaccgc tgctacttaa ttccggtggg aaattattta
     8821 tttccgcata atcagccatg tattatttga aacaattagt ttgtaaataa tataaattga
     8881 taacatatca aatacatcaa aaataccatt cacaggcatt attaatatag acctcaatat
     8941 agcaacatca aattccaatt agtaataaga tatgctttta aattgcatgc aaataatttt
     9001 aaatgagcgt tttttgatac tttagagtgt ttcctaccct ttaatatcac cgtgtagagt
     9061 tgatatcact acaacagaaa catgcatgtc atttgtttac ttctcttttt actacagaaa
     9121 aatttatcta tttttctaaa catcagtgaa atactcataa aaggtaacta ttatgtctat
     9181 gccattaagc aatgcccttc agagtcaaat tattacagat gctcacttcc ttcatcaccc
     9241 aatagtcgac agtgagttta cacgtaaatt aaaatatgca cgcatggatt cggaaaacat
     9301 ctatcttccg cctcttactc gtggcaataa ccataattac gatggaaaaa gcgttgtcga
     9361 aattagaaaa cttgatatta gtaaagagcc ttggccattt aattatgtga caggagcatg
     9421 cagagaatcc gatggtatta caacaacagg acgtatgctc tatcggaact tgaaaatcac
     9481 ctcagcttta gacgaaatat atggtggcat atgtaaaaag gcgcatgcta ccacagagtt
     9541 agcagaaggt ttacggctaa atttatttat gaagagccct ttcgatcctg ttgaggacta
     9601 tacagttcat gaaataacat taggccctgg ttgcaatgtt cctggttatg ccggaaccac
     9661 gataggatat atttcaaccc ttccagcttc tcaggcaaaa agatggacca atgagcagcc
     9721 aaggattgat atctatattg atcagataat gacggtaaca ggggtagcaa attcttccgg
     9781 attcgccctt gccgcattac ttaatgctaa tattgagttg ggtaatgacc caataattgg
     9841 tatagaagcc tatcccggta ccgcggagat ccatgcaaaa atgggatata aagttatccc
     9901 gggtgatgaa aatgcaccac tcaaacgtat gactctgcaa ccgtcatctc ttccagaact
     9961 gtttgaatta aaaaatggtg aatggaatta tattggtaaa taactaccag gaagaatgag
    10021 tactaacatt caggttatac agatagctaa acaataccct gcaatttaca aagtaatact
    10081 cattgcgact tcttaattta agaaagcgca aggaacgttt acccttgcgc ttattataat
    10141 aagtcaggcg tcgactttat ccagcggcca tgaatcaaac agatagccat caaaataaat
    10201 atcgtcaacg ttcgagaatt ccagcagacg ttgcttaacg ttttccagat gctgccacat
    10261 tgccagcttc gccgctcggg catctttttt gattaacgcg gcgaggatct gtttgtgatc
    10321 gcccaaccac tctttgcgat acaggctgtc atccagatgg ctgtgcaact ggatccacat
    10381 tggattgttt tcccgccact gccaggactg acggaacagc tccaccagca tgctgttatg
    10441 cgttgcttcg gcaatagcga gatggaactg catgtcaccg ctttcgctgc tgcccggcgc
    10501 actggaagcc agttcacgct cttccagttg caatgcctga cgcattttga cgatatcttc
    10561 gcgggtagcc tgcaaagcgg caaactcggc gatgttgctc tccaataact gccgcgcctg
    10621 taacagctca aaaggaccgg catcgttgca gacgttggca tccggactgt ctgtgttctg
    10681 gctgcctgag ttatcaagaa catagatacc ggcaccccgg cgtacttcca ccagcccttt
    10741 gatctccagc atgatcagcg cttcacgtac caccgtccgc gtgacatcaa gcatttctgc
    10801 aatttcacgc tccggcggca gccgttcgcc aggattgtac ggcgtcttta tgatcagatc
    10861 gcggatcatc gccccgactt cctggtaagg tctttgcgca gaggtggcag atttcatggt
    10921 gttgtgtccg ttaggtgcgg ttaatcaaat ttttgccgga tgcggcgtga acgccttatc
    10981 cggcctacaa cgggcaattt tacggccccc gttaatctca aactatacca ttagcactgg
    11041 aagttggcac gtacaggtgg gcaaaaaaac gaaaactggt caaccaaata tcagtttgat
    11101 tgaccagtaa gtctgtaggg taatcagtta gttacagcgc agccacacac tcgcgcgcac
    11161 cgcgttcgca cagctgctga tatgccgccg tcactgcgcc aacaaagtcg gcattctgcg
    11221 gcagatcatc ggcaaaaata ccgctcaggc ccagcagcgc tttcacgcgg tctgcgccct
    11281 gatactgcgc gttgatcttc tggaactccg ccagcatcgg gtcgaccacg tcaatggcat
    11341 taccctgctc atccacgccc tgggtgtaac gcatccagcc agccacgccc agcgccaggt
    11401 gacgccagct gccgccgttt tgcaggtgca gacgcaccgg gtccagcaga cgctgcggta
    11461 acttctggct gccgtccatc gcaatctgcc aggtacggtg acgcagagac gggttgctga
    11521 aacgctcgat cagcagcgtc gcataggcgt tcaggtctgt accttccggc atcgacagcg
    11581 ttggcgcttg ttcctgcatc atcagggcaa aggccgcttt gcgataagcc gggttagtca
    11641 cggtgtcggc aatggtttca tagccgccga ggtaaccgag gtacgccaga aaagagtggc
    11701 tgccgttcag catacgcagc ttcatcattt cgaacggcac aacgtctgca acgaactgtg
    11761 cgcccacttt atcccaatcc gggcgaccat taacgaagtt atcttcaatc acccactgac
    11821 ggaacggttc gcaggcaatg gcgcacgggt cgtaaacacc cagctggtca gcaatttcct
    11881 gtatggtttc tggcgtcgcc gccggaacga tgcggtcaac catggtgcac gggaaggtga
    11941 cattttcttc aatccatgcc gccagctgcg ggtcacgcgc ctgagccagc cccagtaccg
    12001 cgaccttcgc cacatgaccg ttttcacgca cgttatcgca ggacatcacc gtaaacgctt
    12061 tcaacccttt ttcacgacgc agacgcaacg cttcgacgat gtaaccaatc gcggacttcg
    12121 gcgcagtcgg gttttccaga tcgtgcttga tcagcgggtt attgagatcc agttgaccgc
    12181 ttgccgcatc agcgcagtag cctttttccg tgaccgttag agagacaatc gccgtttgcg
    12241 gacgcgccat cgcgttgaga ataccttcgc agccatcgat ttccggatgc agcgcttctt
    12301 tcatcgaacc gataattttc agctcggtgc tctctgcgcc tttttccgct acggtgtaca
    12361 gcagttgctg ttttttcagg ttttcgatca gcacgcggtc gttgcctggc atcaggttaa
    12421 cttcgcagat gccccagtcg ctgtcggtgc tttccagcag atgatgggta tacagcgcct
    12481 ggtgcgcgcg gtgaaacgcc ccgcaaccga gatgcacaat gcgtgattcc agacgagaat
    12541 gatcccatga cgggcgggca accggcagat tgctgtcaac aatagtagtc atcgtaaact
    12601 ccagaatccg tcttcaaaga gtgacggcaa ggaagaagga accggcacgg cgcgcagcca
    12661 tgccggtgga tattaacggc taaagaaagc gcgctggatc gccagttcga caccgcgaac
    12721 ttcggccagg cctttcagac gaccaattgc ggagtaacct gggttggttt tcttcttcag
    12781 gtcgtccagc atctgatgac cgtggtccgg acgcatcggg atcaggtctt ctttgccttc
    12841 cgctttacga cggtgttctt cttcaacaat cgctttcacc acttcgtaca tatcaacgtc
    12901 accgttcagg tgcgccgctt cgtggaaggt tttcgggtta tcttcacgca tggtggagcg
    12961 cagatgggtg aagtaaatac gcggaccgaa ctgcttgatc atatcaacca gatcgttgtc
    13021 agcacgcacg ccgtaggaac cggtgcacat ggtaaaaccg tttgccatgc tgtttacggt
    13081 atcaaccatc cactgcatat cttcaatggt ggaaacaatg cgcggcaggc cgaggatcgg
    13141 gcgcggcgga tcgtccgggt gaacagccat acgcacgccg acttcttcag caactggaat
    13201 aatcgctttc aggaagacgg caaagttttc gcgcagcttc gctttgtcga tatctttgta
    13261 cagctccagg tgtttacgga actggtcgag ggtgtagcct tcttccgcgc ccggaagacc
    13321 agcaatgatg ttacgggtca gacgcgcttt gtcttcatcg ctcatagtgg cgaagcgttc
    13381 agctgcctga gcaatttctt cttcggtgta atccgcttcc gcgcctgggc gtttcaggat
    13441 atgcatttcg aatgcagcga attcgatctg gtcgaagcgc agagctttgg agccgtctgg
    13501 cagcacgtat tcgaggtcag tacgggtcca gtcgagcacc ggcatgaagt tgtagcacac
    13561 ggtgcgaatg ccacactgcg ccaggttgcg cagagtctgc tgatagttag cgatccactg
    13621 ctcatagttg ccagtgtggg ttttgatatc ttcgtgaatt ggcacgcttt ctacgacaga
    13681 ccacaccagg cctgcgtctt caatgatcgc cttgcgtttg aggatctctt caacggacca
    13741 tacttcgccg ttcgggatat ggtgcagcgc ggtaaccacg ccagttgcgc ccgcctgacg
    13801 gacatcagct aaagaaaccg gatcgtttgg gccgtaccag cgccaggtct gttccatgat
    13861 ttttcctctt tcacatagtg ggttccacag caaagagacg cggaaccctg gagttgttta
    13921 ggtatccgga tgaacaacca gaccgcagcc gtggcgttgc atcggatgag atggcgtata
    13981 agttctacct tttggtaaga catgtcaacc cgaattctga aattggttaa ccacatcaca
    14041 agaatttcac tctcatggtt gttgctgcaa attgcagcaa agggtaatcg atggttattg
    14101 atcagaggat gtgacattca tcgcaacaat ggttgaccaa tttacataac atatcgccca
    14161 aaataacaac ggttcaacca ggtaaggtaa aacgttatgc atgtgcttaa cattctctgg
    14221 gtggtattcg gcattggtct gatgctggta ctgaatttga agttcaaaat caattcaatg
    14281 gtggctttgt tggtggcggc gctgtccgtc gggatgctgg cgggcatgga tttgatgtcg
    14341 ctgctgcaca ctatgaaagc gggcttcggc aacacgctgg gggaactggc tatcatcgtg
    14401 gtgttcggtg cggtcatcgg taaattgatg gtcgactccg gcgcggctca ccagatagcg
    14461 catacgctgc tggcgcgtct cggtctgcgc tatgtacagc tgtcggtgat tatcatcggc
    14521 ctgatttttg gtctggcgat gttctatgaa gtggccttta tcatgttagc gccgctggtt
    14581 attgttattg ccgccgaagc taaaattccg ttcctgaaac tggcgatccc ggcagtagca
    14641 gctgccacta ccgcacattc actgttccca ccgcagccgg gtccggtggc gctggtgaat
    14701 gcttatggcg cggatatggg gatggtttat atctatggcg tactggtgac gatcccaagt
    14761 gtaatctgcg caggtctgat cctgccgaag ttcctcggca atcttgagcg cccaacgcca
    14821 tcattcctga aagcagatca accggtagat atgaataatc tgccctcttt cggcgtttcg
    14881 attctggtgc cgctgatccc ggcgatcatt atgatctcta ccaccatcgc caatatctgg
    14941 ctggtaaaag atacccctgc ctgggaagtg gttaacttta tcggttcctc gccgattgca
    15001 atgtttattg cgatggtggt tgcattcgta ctctttggca ccgcacgtgg tcatgacatg
    15061 cagtgggtga tgaacgcttt tgaaagcgcg gtgaagagta ttgcaatggt gattctgatc
    15121 atcggtgcgg gtggcgtgct gaagcagacc atcatcgaca ccggcattgg cgacaccatc
    15181 ggcatgttga tgtcccacgg caatatctcg ccctacatca tggcatggct gatcactgtg
    15241 ctaattcgtc tggcgacggg tcagggtgtc gtttcggcga tgaccgccgc cgggattatc
    15301 agtgctgcaa tcctagatcc agcaactggt cagctggttg gcgtgaatcc ggcgctgctg
    15361 gtactggcga cggctgcggg ttccaacacc ctcacccaca ttaacgatgc atctttctgg
    15421 ttgttcaaag gttactttga cctgtcagta aaagacacgt tgaaaacctg gggtctgctg
    15481 gagctggtca actccgtggt tgggctgatt attgtgctga ttattagcat ggtagcgtaa
    15541 aaggacacca gagcctgcca atggcaggct cagactgatg acaaagtcta aaaatgtgcc
    15601 cggacagtcc cctcgcccct ccggggagag ggttagggtg aggggaacag accagcaaag
    15661 tgcgctttgt ttatgccaga tgcgacgctg acgcgtctta tctggcctac aaagggctaa
    15721 cgtgcaggtt tttagcttca ggtaatattg cgtaccagca ttagcaatgt cctgtgattt
    15781 ctttattgat aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc
    15841 cctccggtac gtgcataatt tgccgttaat cccagactca ccgccgaagt ccctactgct
    15901 cctaacgata ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg
    15961 acgccctgtg caggtgaaaa cgacgcggta ttggtgaaaa tcgagttgcc cgcatctgcg
    16021 gttgtgccgg agaggtaata ccccaggttt tggcttttcg cacaataaac ggtaagagga
    16081 attggcactg aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg
    16141 cagccgccag taggcaccac cacatcatta ttggcgtaaa tattccacac aaactggaaa
    16201 tcatcgctgt tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgagccagct
    16261 ttaatcgcca ccccgcccgc actgctcaca ggcgtcaaat aaagcgccac cggccacggc
    16321 ttatccgttc tcgaattata aacaacgcgc ggcgtttcgc tggtggtagg aaatggatag
    16381 ctactgccac tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag
    16441 cctcgttgca gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt
    16501 tgcgtcgaaa gatccacgac caggttttgc cccacattca cgacgggcgc aaggtttaca
    16561 taaacattgg cgctgccacc gccaataggg atagcggtac cattggcggt tttacaggcg
    16621 aatgaccagg catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt
    16681 ttcattacaa tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg
    16741 aatggttccc tgagtggctc cgccatttac tgtcaatgct ctgacctgta acgggaagtg
    16801 cgctgattgt gaggaatcat ccacctgaac tgttttggtt gcgccagtat tcaatgtgtt
    16861 gccactgtca tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata
    16921 tccggtactg tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca
    16981 attagtcaac tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact
    17041 gaaagaataa agatcgccga gatcaaccgt ggcattggtg gtggaaaccg tacacggttt
    17101 ggcgacgacc ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc
    17161 gagcgccaat attgccgcca atacataccc acgtttgcac catttcatga gcatctccag
    17221 ttactgatat tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac
    17281 ctgtgtcgcc attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt
    17341 cgtccacgaa agcgcggacg atggagcatt aaggggtatt tgattttgct gctcattcag
    17401 aagctgtatt cccagtcccg aagccgctga caccgtattt tcaagtgcaa gcaggttggc
    17461 attgtggcta tctgcaacgc cagtaaaccc aacctttacg gcagaaacgg cattaccaca
    17521 gggtgacagc aaaatacgaa atggaacaac aggagtcgtc gcgccaatgt tgttaaattg
    17581 cttcgccgcg ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca
    17641 gccgttatcc ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgtgact
    17701 caccgccagc cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc
    17761 ttaacgacat tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg
    17821 ataattggcg acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc
    17881 taaaggcatt ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct
    17941 actctctgat gtcaccatcg ccccaaacgg cagcggctta ttattgtggg tcagcgtcat
    18001 gagcagtttt atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg
    18061 aacaacgtta gcaaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag
    18121 cgccactcta ttttcccgat attcagtggc ataaggcagc acggcataac cacgccagtc
    18181 ggtacgcacc cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag
    18241 caccaccgta tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc
    18301 accgctgact ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatattggc
    18361 attgccgtaa ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tatttccatc
    18421 gcctcccccg gcatagccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt
    18481 accgtataca ccagccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtagct
    18541 ggcactggca tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat
    18601 attgacgtta agcgctaaca tctgatcccg tcctttttgc caggcgtttt tcgtcaggct
    18661 atagctgagc gtccagttga tatcttcgaa cgcagtattt aatccagcct ggaattgctc
    18721 atcgacatta ctcgttcccc aataagtttg atggctacca ctcaaataca gtgttgatgt
    18781 gcgcccgagt tgctgagtaa cggtgagttg taatttcccg cgtttgttat aagcgaggtt
    18841 gtaatagtcg gtgaatttcg gcttaacctg aataactccg tcctgtgttt cgatgttgta
    18901 gccattcatt cgactgtatg ttgtatcagc gaaattaaaa tatccgctgg tcgaataacg
    18961 gtaacccact aactgaatat tcgtgcctga ttcattgagc gatttgttat agagaaaacg
    19021 caccgattgt ccgtcatgct gactgtcatc gggaagtgtg gaattagcct gcgtcatatc
    19081 cacagacaga gcgcccagtg cccccatgtt tttcccgata ccgaaattaa aagcacgata
    19141 acgatccgcc agttgcgttc caccatatat tgtccagcca gccggaaggc cgtggagtaa
    19201 tgtactctgg aaaaagcggg gtttttcctg ctgcgcattt ccactacggt attctcctgc
    19261 cgtaatggaa taacgagtat gcccttcacg ttgcaaaagc gggactgacg aatagggtac
    19321 ggtaaaaatc tgcgtgctgc cgtcagcctc tttgatcgtt acctgcaagt caccactatt
    19381 acctgcggca tagatatcgt tgatggtaaa aggccccggt ggcaccgtac tattataaat
    19441 gtcataccca ttttgtttaa tagtgacctg tgcagtacca cgagcaatac cgtggatcac
    19501 cggggcaaat cctctttgac tatcgggtaa catattgtca tctgaggcca attgtgcgcc
    19561 gcgaaagtta ataccatcga aaatatcgcc ctgagtataa ccatcaccca gcgtcagccg
    19621 ggaacgtaac ggtattatgt ctcgctcaag ccaggtattg atatgctgcc atttattttt
    19681 gctacctgat gatctgtcgc tactgttata actccaggtg gtattgtcgc gtaaacgcca
    19741 cgcaccaata tttaacccac tctgtaggtt taaatatgca taatggctgt tacccccaat
    19801 ccgattctgt acactatttc cgctgaaatt ataattgagc aatccggcat taataccggg
    19861 atcccataac tcaggaggaa tataaccacg cgcgcgatta ctcataaatg cctgagggat
    19921 cgtcaggttc agtcgctgct gaccaacatc cagatgcgca gtagcgtcct ggaccattgt
    19981 ggttaatggc acacaggcat catccgccag cagattcata ccggcgacag aagccgtatt
    20041 cagccccata ctggcgagtt gcgcgcgtgt caggcaggga acaatccctt gttcactgtc
    20101 gcccgtatta aatgtgacat cacgcgttgc catataacca ttattcaaat agatatcgac
    20161 gcgatatgtc cctggcggta attcttgccc attttcaaaa cgcgataaat cggccacagc
    20221 ctggggatca tccgctaaaa agcgcggatt aaaatagagg tcggcagatg acaaaggtgc
    20281 ctgtgcggca aaagcacagg cgacaacgag tcggacaaaa aaaccagcca aacgatgctt
    20341 acgaatatgc aagcattgtg tgtttcgctg gtaaagtctt aaattcagat atgacattat
    20401 tcctccggct taaaatcacc cggcagtcaa ttctttttat tcaggcgaaa aattccccct
    20461 gcgttattcc attacgcccg tcattttggg ggtaagtgcg ccataatcat ttattgttcg
    20521 gtaagtaata ttgcttcctg catcagaagg caatttaacc gtgctttcgc ccattggagg
    20581 caccaatgca ttttcaagaa cccgggttcc ggcattcaac tctgttaccg tcaggtaata
    20641 gggtgtcggg ttaatcagcg tcagagaatt cgcgctacga cgaaatctta atttttctgc
    20701 ggcctgatcg ggtggcaacg ctaatttagc cgggcgatag tacagtttaa tgcggctgat
    20761 aattgcgagc tgtagcgtat tctcagtcaa ttttgattta tccattgacg gaatcgcttt
    20821 aacgttcatc cagaataaac tttcccggtc ctgtggcaat tggttatttg ttgcatcaag
    20881 aatacgtaag gtattctctt tttttccctt catcgcaaac agaggaggcg tcacgataaa
    20941 acgaccatcc tttacaccat cggcattttc cacccatgat tgaattaaat aggtactatt
    21001 ttcatcatta tttgtcacgg caagttgcac ttgtttttgc cctgccggat aaattacgcg
    21061 agtcgcacct aaggccactc ccgcttcagc gcgtccggca accatcattg ccatgaacat
    21121 caggatacct gccagcaagc agaatgttat ttcctgcgat ttccttacat tgacgttttt
    21181 attactcact gtcctgttcc tgttatcaca ttatctgctg aacaattact gataggttaa
    21241 agagaaccag gcctgggcat tggcgatgcc gccagtaacc cgacgcccgg tagcacgata
    21301 tttggcgatg aaatgtagcg aagtagagcc tgaataaagc cgtttccagt ttgctggagg
    21361 acgattaatc ggtacgaggt ttccttcatc atcaaacaac gctacgccaa tattggtggc
    21421 tatccctggc ccctctccca cggaaagcac atccggattt ttaccatccg cgacaccgtg
    21481 aaacgccaca cctacacgtt cactcaccac cgtgctacat tcccgtaaat gaataacaaa
    21541 aggcaccggt gcgctatctt ccccaaccgc atgaaaccgg ttactgctga tttgccccat
    21601 attgaccgtc atttgtttat caccggcttc aatccggcaa gtttccgcaa taatgacgcc
    21661 ctgaaattgc atatttccgc cgggcaacgt ggtattccat ttatttccgg ccagagcaaa
    21721 cattggcagc aacgacgcta atagaaataa tctcttcctt ttcatcgtta tttttatcgc
    21781 acaagggtgg gcatccctgc ccgtaatgac gtccctgaac ctgggtaggt tattgatact
    21841 gaaccttgaa ggtcgcatcc gcattagcag cacccggggt tgcggccccg gttgcaaaat
    21901 aacgcgcctg gaacggaatg gtattggttc cgttattcag ggttgtttct gaactaaatg
    21961 tcgcaccatc cagcgtcagc gcagcacccg ttctgtccag gatctgcaca ccaacgtttg
    22021 ttgcgctacc cgcagctgaa ctctgcagag ccagaacgtt ggtatgaccc acatcaatcg
    22081 ccgtacctaa aaaggcaaca gcggctttag atgcaacatt ggtatcgcaa tcattcagct
    22141 gaatgttaaa accggcagca gaactggttg ctccttcctg tgccagcgat gcggtacgaa
    22201 cctgtcctaa ctgaacggtt tgatcaacag agcctgcatc aactgcgcaa gcggcgttaa
    22261 caacttcccc tttaaagtga acggtcccac cattaaccgt cgtggcagcg gccagagccg
    22321 ctgtagaact gagggacaga gccgacagaa caacgattgc cagagtttta attttcatgc
    22381 tgctttcctt tcaaaaaact atttctaaat cgacatgggc agtcgttctg tacactttgt
    22441 tttgtcaacg agtttgactg ccaacactgc acagttttcc cccaaaagat gaaacatttg
    22501 gggccatttt gactcataga ggaaagcatc gcggacaaac tttttcagtt tatttgttgg
    22561 cttaatattc tattgttatc tttatttata gatgtttata ttgcatgagg tggtttttgg
    22621 agagaagaat gaggaagatg cgtcgagcca cagaaacgtt agctttacat atagcggagg
    22681 tgatgtgaaa ttaatttaca atagaaataa tttacatatc aaacagttag atgctttttg
    22741 tcgtttttaa tatttttatg cttgagaaaa aatacgtaac ttatttatga tatggacagt
    22801 ttggccccaa ttgtcttgta tttatttgtt tttttaactt tattatcaat aagttaaatc
    22861 aaacctcttc tctttttaat ttttcgttta tgagattatt tctttcccat aatccggcaa
    22921 aacgagcagc attactggcg gtataacgca cagtatggcg aatatttcga tgcccgagat
    22981 aatcctgaat taaacgagta tctgcaccac gctccgccaa ttcataaccg caagcatgcc
    23041 ttaacatatg aggatgagtc tgcgttacgg ttccagcttc aataccggca tcgcgaataa
    23101 tgcgataggc ctgctggcga gaaagccgac tcccgcggcg agaaataaat atagcgtcag
    23161 tccggtcagc gcctttccag ttagcacgtt cctgggtcca gcgttccacg gcttcacgct
    23221 catcaaaacg taacgggtga acggtagaaa atccgttctt cagtcggcga atatttattc
    23281 taccttcatt aaggtcaagg tcctgataat gcagatcaag cagttcacta atacgcatcc
    23341 catgccgata tgccaacaga ataagacaat aatctctggc tcccgttgcc ccgtaacaaa
    23401 ccgcctgcat catggcctga acttctttac cggtaagata acgacgttta ctcacaatag
    23461 tagtactcct aactgagata tatttgaatg aatacctata ggaaacctca atcggtcaaa
    23521 attagcccaa tagataaaaa gataaccaca cacggagtga tgtggctatc agtcaattac
    23581 attaaatatc atacaaaaat aaaatatcac tgatgtgaca acatctattt tcattgattt
    23641 gattcaatat aacaatagat tgcagtggat taacttccag ttgcttaaaa aataaagaaa
    23701 aaagtgcgga aacaggagat tttaagtgat gtgtaaattt atgaatgatt ttgtttattt
    23761 attttgaaga acataaacca ttgtattttg tatggatatg gttaacttaa tgtttattat
    23821 gataattaag aatcacactt acaataacat caattaataa atggttaatc atttttgata
    23881 tatcgtaaga ataatgtagt ttttaacacc atccctggta tctcaactat ctctataaaa
    23941 cagcgtgacg ctgtcgtcct ctggctctat cccagatgcc gtaaaaacgc cctgcattgc
    24001 tggcggtata ccagacagta tgacgaatat tgcgatgccc aagataatcc tggataagtc
    24061 gcgtatctat tcccatattc gccaaagcaa aaccacacga atggcgtaac atgtgcggat
    24121 gaatctccag tgacaacccg gcattaccac cggaagtcga gataatatgg taaaactgtt
    24181 gccgagaaag cggattcccc ttacgtgata aaaataccca ctcgctctca gcatgcgggt
    24241 acgaagtacg gatactcaac cagtttttta aagcctgaac ttctttattc aatagcgggt
    24301 gcgttgttga aaagcctttt tttaatcgat ggatatatat acactttgcc ttaagatcaa
    24361 tatccgaaat cctcaatcga caaatttcac tcgcccggaa accatgaata aaacaaagca
    24421 aagtcagaca ataattacgt gctgcatgag gcccggtatt tgctgcttta aggagtgatt
    24481 cgatttcact atgggtcagg aagttccttt ttttgttatc agccttattc ttcatcgttt
    24541 ttcccttata attacagacg cgcactagct gtgctgggtt agcaataatc caacattttt
    24601 atcctgatta tgtttataaa agcgaacgtt tgcttaataa ctaacgtaag tggaccagtt
    24661 cttctgagtg aacttaaatg gagtagcaac tgttaattat aataacgttg ccatacaaac
    24721 gccatgctct tgcatgctat gtacctttat atatttatca atcggatacg aatctaataa
    24781 taaccctcat cattaatgca actaatctta tctatatatc atgtgatatg ttctgtaaca
    24841 agtaaaatca acataaaaaa ttcagcaaaa tttaatattc gttcaactag ccgtctcaca
    24901 tatgacatta ttagtcaact ccatttgcca caaaatggct atttcatgga gcgcagtaat
    24961 ttcttagtgg ctattatcat gctaattctt aggtccctag cgattattcc tgctgattcg
    25021 tcagcttacg ccatgagatt gttacattcc tttttatcct ggagatatgg gggaacgcag
    25081 gagatgattt tttcatctac attcgttggt aaatatagct ttggtcacta cgacttaaac
    25141 cttcctcaat cacagccatt cgccgggcga tacggcaaac aaactaagtg aaacatcatc
    25201 ctgaaaacac caacatcaac aagcctctcc agatcgactt cagaagtgac cagttacaag
    25261 ccacaaacca ggaaacatat ttcatgatga gaattatgct cagcctgatg gcgggtaaac
    25321 actgattttc accaagctca ctgagttagc ttgcaaagct ccccttgttt agactctaaa
    25381 caaattattt ataaaacaaa tagttaaact aagatcttta gtttttgaag accaccgcaa
    25441 gtgttcgtct ggcttcacat ggcatcttcc tcttagaaaa agatcgacat attttgtgac
    25501 acgaattgca aatctggttt tgttgtatgg attgcgtgat ttttgatctg gtataacagg
    25561 tataaaggtg caccaagata gtcaatgaga cagggcatct cgcaatctat ggcaaacatc
    25621 acttcagttc tttctcatcg ggtgatgaaa acgcacttca gtctgaaagg aatatgaaaa
    25681 tgagatcaac agacattcta ttttatgact ctgggtaaaa tggattgagt aagtgatata
    25741 gcttacgaac attcaaatca attaaacatc agaagagatt ttatactcag gtatttaatc
    25801 tggatctctg tttatttaaa taatgtgaaa agagattttt cacaggagac cttatacaaa
    25861 aaaatataaa atacagctac cggttgccaa agacactata agcctggcaa aaaaatatta
    25921 cacaacataa atgctaattg tttatgcggg ctttgtattg ctttctgtat cctacaaatg
    25981 agtgaaattt atgaaaaagg ctaaaatact ttctggcgta ttattactgt gcttttcgtc
    26041 cccattaatt tctcaggctg cgacactgga cgtacgtggt ggatatcgta gtggaagcca
    26101 cgcctatgag actcgactca aagtcagtga gggatggcaa aatggatggt gggcaagcat
    26161 ggaaagtaat acctggaata ccattcatga taataaaaag gaaaatgccg cactcaatga
    26221 tgttcaggtt gaagttaatt acgcgattaa acttgatgat caatggacgg tgcgcccggg
    26281 aatgttaacg cattttagca gcaacggcac acgctacgga ccctacgtaa aactgtcctg
    26341 ggacgcgaca aaagatctta attttggcat tcgctatcgt tacgactgga aagcttaccg
    26401 acaacaagac ttatccggtg atatgtctcg tgataacgtt catcgttggg atggatatgt
    26461 cacttaccat attaatagtg atttcacctt cgcatggcaa acgacgctat acagcaaaca
    26521 gaacgattat cgctatgcaa accataagaa atgggcgacg gaaaatgcat ttgttctaca
    26581 ataccatatg acgcccgata ttacgccata catagaatat gactaccttg accgtcaggg
    26641 tgtttacaac ggcagagata atttatcgga aaacagttat cgcattggtg tgtcatttaa
    26701 actgtagtag acaggagaca gtcacaatga ataaaacaat aacggcgctt gctatcatga
    26761 tggcttcatt tgccgcaaac gcgtctgtat taccggaaac tcctgtgcca tttaaaagtg
    26821 gtaccggagc aattgataac gacactgtct acattggttt aggtagcgca ggtacggcat
    26881 ggtacaagct ggatacacag gccaaagata aaaaatggac agcgttagct gcattccctg
    26941 gcggaccaag agatcaagca acctctgcat ttattgatgg caatctgtat gtgtttggcg
    27001 gcattggcaa aaacagcgag ggcttgactc aggtatttaa tgacgtacac aaatacaacc
    27061 ccaaaaccaa tagttgggtt aaattgatgt cgcacgcgcc gatgggcatg gcgggccatg
    27121 tgacttttgt acacaacggc aaggcttatg ttactggtgg tgttaaccag aatatcttca
    27181 atggctattt tgaagatctc aacgaggctg gaaaagattc aaccgctata gataaaatca
    27241 atgctcacta ttttgacaaa aaagcagaag attatttctt caataagttt ctgttgtctt
    27301 ttgatccctc aacacagcaa tggagttacg ctggcgaatc gccctggtac ggaacggctg
    27361 gtgcggcggt tgtgaataaa ggtgataaaa cctggcttat taatggcgaa gccaaaccag
    27421 gattgcgaac ggatgccgta tttgaacttg atttcaccgg taataattta aaatggaata
    27481 agcttgctcc cgtctcatca ccagatggcg tagctggcgg ttttgcgggg ataagcaatg
    27541 attctcttat atttgccgga ggggccggat tcaaaggttc acgagaaaat taccagaacg
    27601 gtaagaacta tgcgcatgaa ggcctgaaaa aatcatatag cactgatatt catctttggc
    27661 ataacgggaa atgggataaa tcgggtgaat tatcgcaagg tcgggcctac ggagtatcat
    27721 tgccctggaa taatagtcta ttgattattg gcggtgaaac tgcaggcggc aaagcggtga
    27781 cggattcagt tttgatcact gtgaaggata ataaagtcac agtacaaaac taacgcttca
    27841 gggccccggt aaaggggccc tgctatcaat ttgtcatttc aattacgcga aatttatatg
    27901 aacgcaataa tatcgcccga ttattactat gttcttaccg ttgctggtca gtctaatgcc
    27961 atggcgtatg gcgaaggact gccattaccg gacagggaag atgcgcctca tcccagaatt
    28021 aaacaattag cgagatttgc gcatacgcat cccggaggcc cgccatgtca ctttaacgac
    28081 attattccac tgactcactg cccacacgat gttcaggata tgcagggtta tcaccatcct
    28141 ctggcaacga atcatcaaac acagtacggc accgttggcc aggcactgca tattgcacgg
    28201 aaattactgc ccttcattcc tgataatgca gggattctta tcgttccgtg ttgccgtggc
    28261 ggatcggctt ttaccgcggg cagcgaaggg acatattcag aacggcacgg agccagccat
    28321 gatgcttgtc gttggggaac ggatactccg ctgtaccagg atttagtcag cagaacgcga
    28381 gccgcactgg caaaaaatcc gcagaacaaa ttcctcggcg catgctggat gcaaggcgaa
    28441 tttgacttaa tgaccagtga ctacgcgtca caccctcaac actttaatca tatggttgaa
    28501 gcctttcgta gggatctaaa acaataccat tctcagctta ataatattac tgacgcgccg
    28561 tggttttgcg gcgataccac ctggtactgg aaagaaaatt tcccgcattc gtatgaagct
    28621 atttatggca attatcaaaa taatgtttta gccaatatta ttttcgtcga cttccagcaa
    28681 caaggtgaaa gaggactgac gaacgcgcct gatgaagacc cggacgattt aagcacggga
    28741 tattacggtt cagcgtaccg gtcaccggag aactggacga cggcactgcg aagcagtcat
    28801 ttcagcactg cagcccgtcg ggggattatt tctgacagat ttgtagaagc aattttgcag
    28861 ttttggcgcg aaaggtgagc ggggagttta ttcatggggg atgtccttgt tcgttctcag
    28921 agtagacaac aaaaaacaaa tgagatcaag gtaatatctc ttcacttgcg ggctcgaaca
    28981 gcaggcaatt gccaatataa taatctggca caagggtgaa aaacgcgcac cgcataaacc
    29041 gttgttattg cttacagatt agccggatgc cttaatagac atccgcgcct tttcgattat
    29101 ggctcggaaa tctacgaaca tctacacagt ttactggaac gttttgggcc acagcgttcg
    29161 cagcaccgtc ctgatatgcc gttctggcga ttataagtcg acggattctg gcaaatagac
    29221 gatgatgata tgcttaatga tgttcaaaga aattaggcca ttattatatt attgcttgca
    29281 ttttctttca ttcctaaaag cacccgccat taatcccagg cgcattatgc aaaggaaaat
    29341 gattaggtaa acaataagat ttataatcag gaaaggcgac tcgcaataaa acggaaagtc
    29401 gccttaacat tggtaaaata tgccctttac tactcctgta tcatgaacgg ctcatcacat
    29461 ttaaagctga cgttgatcca gactaccata tattgccttt atttcatttt ttacgccttc
    29521 gccacggtac accaaagacg tatcgtatcg ttgatactta tcttcccgtg tagcactgaa
    29581 ccagttaaaa gatccgacac acagcagttc ttcatcctca atcacaattt tgctgtgaac
    29641 acgattaacc aatttcgtag cgatacccat cttattgagc ttttcaacag catcattcag
    29701 cagatgttgt ttctcctgac gcttatcatc atcgacatgc gctatattgc agtttttatc
    29761 cgtcaccacg gtaatatcaa tgccccggga acgggccaaa gccattgatg ccagaaatcc
    29821 cgtttgctcc actttttgcc aggataacca aggggaaata atcgttattt tcttttgagc
    29881 tccggcaaga gtcttgttca aaaattcatc atgctgctcc acaccatgca gcgttgaaat
    29941 ttgtgtatgt gcggaaatta aatcctgtcg tttctgaaac tcaaattgca acgcattatt
    30001 atctgaagaa aagagatatt ttgccaataa ccctcgcggc gaaaatgcgg gctgcatctc
    30061 gataaggtcc atatcgccaa ataccagaaa actatcttta gcgcgtgaga cagcaacatt
    30121 gaggatagtg ctgttgctat caagaaacct gccatcttca tgttttgagt agacaggaga
    30181 aaagagaaca atcgcccttt ccgccccctg caaggagtgc acagtcccca ctgtcagtaa
    30241 accctgctcg tctttgccat taatttccag cttacgcaat gacattttga tagcattaac
    30301 ctgcgccgaa aatggcgtca caacaccaac aactttgtac agtggttccc cgtaatggcg
    30361 ctctatatca tccttatgtg ccaccagcca tgctgctatt gtttcagctt caagagggtt
    30421 atgacggctc cctccatttg gcttcatgcc tctaccatca atatgtaaat atcccattgc
    30481 ggggaaaatc gtcctttttc catccctctt ttaggctgca acttaccgtg atagcaaagt
    30541 gagttacagt agtcgatgat gttattgaaa cagcgacgat gctcatacag atacattccg
    30601 cgctcgaact tgggatcata ctggtagtga gagttgaact gggctacctg cattacgctg
    30661 ccagatgctg cactttttcc agacccattt attatcgaat acggtgaccg cctgagcggc
    30721 cacctttaat cgtgggcagt tacacagaat tatttacagg gtcttgctgg gagttcttac
    30781 acttgctcgc gatattcaat tggccattgt caacaaatga cgcaggtttt gattggtgat
    30841 atctatttac gtcagcaatg cgatgtgttt tggctcggct ataccatcag tcccgcttat
    30901 gctcgccagg gatatgcaat tgaagcaatt accgccacga ttgactgaat aaaagagaac
    30961 ggtttttcac tcatcaaagc cggtgtgaat ccggaaaata ccctttcaaa aaaattactt
    31021 atcaggattg gcttcaattt ttcaagcatc gaagatgggt gagcatattt atgttctgga
    31081 tttaacaaaa ggagaataat ctcccgaagg gaaaagccga tgaggccatt tcaggcgtac
    31141 ttcaacatgc attcgtcagt gtcatctgcc ttttccagta aatgggttgg cattcaattt
    31201 ccaaagtaca tcacactttt tctactgaaa catatccctc ctataactac gtgttaatat
    31261 tcgctccgta aatacctttc aactcaaccc gaggctttga tgctggagaa tgtaatcatc
    31321 cgataatcag ttccccgacc ttttcaggcc ggactgatta tcaatgcgcc gaaatcgaat
    31381 gcggacaccg cggtgtgttt gcacgttttg cacatgatga ttaaataggt tcttcagtat
    31441 gaatttatcc cgtcaggaac aacatacctt acacgttctc gctaaaggta gacgtattgc
    31501 gcacgtccgc gattcttcag gccgcgtcac ttccgttgaa tgctacagcc gcgaagggct
    31561 gttgctgacc gactgcacgc tcgccgtctt caaaaaactc aaaaccaaaa aacttatcaa
    31621 gtccgtcaat ggccagccct atcgaatcaa caccaccgag ctgaataaag ttcgcgctca
    31681 gctcgataat cgctaaggag agtttctatg acagtacacc acttcacttt tcatatcaca
    31741 gataaaagtg atgccagtga tatccgggaa gtcgaaaccc gcgcatttgg cttcagcaaa
    31801 gaagccgatt tagtggcctc tttacttgag gatgaaagcg cgcgccccgc gttatcgctg
    31861 ctagcccggt atgaaggcaa agccgtcggg catattttat ttacccgggc aaccttcaaa
    31921 ggagaaatgg attcaccgtt gatgcacatc ctcgctcccc tggcggtcat accggaatac
    31981 caagggatgg gcgtgggagg acggttgata cgcactggca tcgagcacct gaggttgatg
    32041 ggctgccaga cggtttttgt tctcggccac gccacctatt acccccgaca tgggttcgaa
    32101 ccctgtgccg gagacaaagg ctatccggca ccttatccca tcccggagga gcacaaagcc
    32161 tgctggatga tgcaatcact caccgctcag cccatgaccc tgaccggcca catccggtgc
    32221 gcagaccctg atgaaaccgg agcactgacg tgaataacaa tcttgttgtg tcctttttaa
    32281 ggcttaaccc aataacagga attaaataat ggatatccca cgtattttta ccatcagcga
    32341 aagtgaacac cgcatccata acccgttcac cgaagagaag tacgccacgc tgggtcgcgt
    32401 gctgcgcatg aagccgggca cccgcattct tgacctcggc agcggctcgg gcgagatgct
    32461 ctgcacctgg gccagggatc atggcattac ggggactggc atcgacatga gttcgctctt
    32521 caccgcgcag gcaaagcgtc gcgcggaaga actcggcgtc agcgaacgcg tacattttat
    32581 tcataacgat gcggccggtt acgtcgcaaa tgaaaaatgt gacgtggcgg cctgcgtcgg
    32641 cgcgacatgg attgccggtg ggtttgccgg ggcggaggag ctgctagcgc aaagcctgaa
    32701 accgggcggg atcatgctta tcggcgaacc ctactggcgt cagctaccag caacagaaga
    32761 aatagcccag gcctgcggcg tcagctcgac aagcgatttc ctgacgctgc caggacttgt
    32821 cggagcgttc gacgatctcg gctatgacgt ggtggaaatg gtactggcag accaggaagg
    32881 ctgggaccgg tatgaagccg caaaatggct gaccatgcgc cgctggctgg aagcgaatcc
    32941 tgacgatgac ttcgccgccg aagtcagggc cgaactaaac atcgcgccga aacgctacgt
    33001 gacctacgcg cgggaatgct ttggctgggg cgtgttcgca ctcatcgccc ggtaacaaca
    33061 ggttcccggt atttctggct ggaaatatcg aggctttacc tttgctttga tctgatgtat
    33121 cacctgcaca atacatattc agtatgttta ttaatcaaga tgtcaccata aggagcatca
    33181 tatgtcacaa catgaacagt tctgtcaggc ttgcggaatg ccgatgtcgg caccagatgc
    33241 acacagtgcc agtgatcaat attgtgcgta atgcagcgac agcaacggca atctcaaacc
    33301 ctgggaagaa gcggtatcag gtttagccag cttccttgat tcctggcaaa aagtcggccc
    33361 ggaggaagca cataagcgtg caaaacgtta tctgacggca atgccagcct gggctcataa
    33421 agcggagagt gatttgagct gattatctat actccctctg aatctattca ggagatccga
    33481 tgttttctta cattatgctg ggaaccaacg acctgccacg cgccattaca ttctaagatc
    33541 cgctgatgga gatgctgggg tatccacgtg caggacgtaa tgaagaggga gcgtcctgca
    33601 acaaagtggt gctataacag ttaccgcgtt ttattcatcc tcattatttc aaccataatc
    33661 actggtctct ccagcccgta actttcacaa acgttgagcg cttttattcc actcggctca
    33721 cataattttt tatttgttca gctttgcgcg ttttcacgtg aacgttctca caattattcg
    33781 accagtctgc gcctataaaa cgctcaacga gtgattgttt tacggtctac ggagatagct
    33841 atgtcatttt ctgtgcagga aaccctgttc tcgcttttgc agcacaatgc gatttcagga
    33901 cacgaaaacg ctgtcgctga cgtcatgctg tgcgaattca ggcgtcaggc aaaagaggtc
    33961 tggcgagaca ggcttgggaa tgtcgtcgcg cgctacggta gtgataaacc cgatgcgctg
    34021 cgactgatga tttttgcgca tatggatgaa gtcggtttta tggtgcgcaa aatagagccg
    34081 tctggatttt tacgctttga acgcgtaggc ggtcctgcgc aggtcactat ggctggttcc
    34141 atcgtcaccc tcaccgggga caaagggcca gtcatggggt gtatcggcat taagtcctac
    34201 cactttgcca aaggcgacga gcgcacgcag tcaccttctg tcgacaaact gtggattgat
    34261 attggtgcca aagacaaaga cgacgctata cggatgggca ttcaggtcgg tacgcctgtc
    34321 actctgtata acccgccgca actcctggca aacgatctgg tgtgcagtaa agcactagac
    34381 gatcgtctag gctgtactgc cctgctcggt gtagcggatg ctatcagtac tatggagctt
    34441 gatatcgccg tttatctggt ggcttcggta caggaagaat ttaatatccg cggcattgtt
    34501 cccgtattac gccgtgtaaa acctgacctg gcgattggta ttgatatcac tccatcgtgt
    34561 gacacccctg atttacacga ttattccgag gtcaggatta atcagggcgt tgggatcacc
    34621 tgcctgaact accatggtcg gggaacgctg gccggattaa tcacgcctcc tcgcctgata
    34681 cggatgttgg aacagacggc tcttgaacac aacattccgg tgcagcgaga agtggctccc
    34741 ggcgtgataa cagaaaccgg ttatatccag gttgagcagg atggtattcc ctgcgccagt
    34801 ctctctattc cttgtcgcta tacccattct ccggcggagg ttgccagcct gcgtgatttg
    34861 actgattgca tccgcctatt gaccgctctg gcaggtatgt cagcagcaca tttccccgtt
    34921 gagcctgatt caggcactac acaagaggca catccattat gaaaaagatc cttgtggcat
    34981 gcggtaccgg catgtcgact tcaaccatga ttgcacacaa gctgcaagag ttcctgactg
    35041 aacagggcat ttcagccact accgcccaat gctgtctgaa tgagatccct cttaactgta
    35101 acggtatgga ccttattgta acctccatgc gaaccaacag tgattacggc atccctacgc
    35161 ttaatggcgc cgccctgctc acagggatca acgacgacgc gttaaaacaa caaatcaagg
    35221 ctctgttaac tcaataaaag gaggcattat gtttgattac atcctgtctc tcggcggtac
    35281 cgttttcgtc cctatcatca tgatcgtgat tggccttatc ttccgcatcc cctggctgca
    35341 ggcgattaag gcaggggtaa cggttgggat tggttttgtc ggtatgggac tggtgatcgt
    35401 catggcgatc gacagcctca gtccccctat caaagtgatg attgagcgtt tcggcctagc
    35461 cctgcatgtc tttgacgttg gcgcaggccc tgcctccggc gttggttatg cgacagccat
    35521 tggcgccatg attatcccag ttatttttct tctcaatgtc gccatgctgg tcacccgtct
    35581 gaccaaaacg atgaacgtcg atatttacaa ttactggcac tacgccatta cgggtacggt
    35641 ggtccagctt atgaccggta gcctgattta tggtgtgctg ggggcaatct gccatgcggc
    35701 gctatcgtta aaaatggccg actggacggc aaaacgggta cagaacatcg tcgggctgga
    35761 aggtatttcg ataccgcaag gctacggttc cagttcagta ccgcttttcg ttctacttga
    35821 tgctatctat gaaaaaatcc ctttcatgaa gggacgcaat atcgatgctc aggagatcca
    35881 gaaacgctac ggcatggtcg gcgatccggt gattatcggc gtagtgctcg gtctgatttt
    35941 tggcctggcg gctggtgaag gctttaaagg ttgcgccagt ctgatgatta ccgttgcggc
    36001 aatcatggtg ttgttcccgc gcatgatccg cctgattgtt gagggcctgc tgcccatctc
    36061 cgacggtgca agaaagttct tccagaaata tttcaaagga cgtgaagtgt atatcggtct
    36121 ggatactgct gtgacgctgg ggcatccaac caccattgct gtcggtctgc tactgatacc
    36181 cattatgtta attctggcca gcattttacc gggcaacaaa gtgttacccc tcgcagacct
    36241 gccggtagca ccgttcttta tctgtatggc tacggttatc caccgcggag acctggtcag
    36301 gacgttgata agcggggtca tcgtgatgat taccgtcctg ctgatcgcca cccagttcgc
    36361 gccttacttc actgaaatgg cgctcaaagg cggtttcagt tttgccggtg aaagtgcgca
    36421 aatctcagcg ttgtccgtgg gcaatatgtt cggttggtcg atttctgaat tgatgtcgct
    36481 tgggattatt ggcgttgtcg tggcggtcgg tattgtcgcc agcgtcgtgc tgttcttacg
    36541 taaacgtgaa ttatcggaat aaggagtact ttcaatgagt tggctgaaag aggttattgg
    36601 gacggaaaaa gcggtcatcg ccatgtgcca tctacgtgca ttgcccggcg acccgagttt
    36661 tgatgcgcag ttggggatga actgggttat cgacaaagcc tgggacgatc tgatggcgct
    36721 ccaaaatggt ggggtggatg cagtcatgtt ctccaacgag tttagtcttc cctatctcac
    36781 gaaggtgcgt ccggaaacca ctgcggcaat ggcacgaatc atcgggcaat taatgagcga
    36841 tatccgcatc ccgtttggcg tgaatgttct gtgggatccg gtcgcctcat tcgacctggc
    36901 gatggctacc ggcgctaagt ttatccgcga gatatttacc ggtgcctatg ccagcgactt
    36961 tggcgtctgg gacactaacg tcggtgagac catccgtcat caacaccgga ttggtgcagg
    37021 cgaagtgaaa accttgttca atatcgtccc agaggccgcc gtgtatctgg gcaatcgcga
    37081 tatctgttct attgctaaat cgacagtatt taacaaccat cctgatgcgc tctgtgtttc
    37141 tggcctgacc gccgggacgc gtaccgacag cgcgctgtta aaacgggtca aagaaacggt
    37201 gcctgacacc gtcgtgttgg ccaacacggg cgtctgcctg gaaaacgtgg aggaacaact
    37261 cagtatcgcc gatggctgtg tgaccgcaac cacctttaaa aaggacggcg tttttgccaa
    37321 tttcgttgac caggcgcgag tcagtcagtt tatggagaaa gtgcatcata tacgccgata
    37381 agatggggat aagcagagcg cttatccccg gcaaagagca ataacccttc tgcgatccct
    37441 gccgccttcg tcaaaagtat cgacggggat cgctaccaga ttaacccgaa tcggcatgga
    37501 gtcaacgacg gatgattaac gatattaaat gggtacaggc acaacgtaaa gccaccgact
    37561 ggcgtcaggc cgtggaaatc gccacccgcc cgctggtagc ctatggtgcc gcgcagccat
    37621 gctacgttaa tgggatcatc gaaaataccc ttaactgggg gccttattat ctgatagccc
    37681 caggaatcgc cttgcctcac gcccgaccag agcaaggcgc aaactataat caggtcagta
    37741 tcaccacgct acgcaccccg gtcgcctttg gtaacgagga gtgcgacccc gtctggctgc
    37801 ttctctgcgt cagcgccacg gatgccaacg cacatatttt gaccattcag cgcatcagcc
    37861 agtttattga ttcgccacaa cgcttaacgg cagtgggaaa tgccagcaca gacgatgcac
    37921 tgtttgcgct ggtatcaggt taacggaggc gcttatgatc ctgcaccctt ctctcgccag
    37981 cgcaaatcct ctgcactatg gtcgtgagct gacggcattg gacaatctgg attttgggag
    38041 cctgcatctg gatattgagg acagcagctt cattaacaac attacgtttg ggatgaaaac
    38101 ggtgcaggcc gtcgccagac agacgcctca tcccttgtcc tttcatttca tgcttgcccg
    38161 gccacagcgc tggtttaacg cgctggctga aatcagaccc gcgtggatct tcgttcatgc
    38221 agaaacgctg gactatcctt cggaaacgtt aactgagatc cgccataccg gcgcacgagc
    38281 cggactggta tttaatccgg caaccccgat cgacgcctgg cgatatctgg cgtctgagct
    38341 ggacggagtg atggtgatga ccagtgagcc cgatgggcag ggacagcggt ttattccctc
    38401 catgtgcgag aaaatccaaa aagtacgcac ggcttttcct cagaccgagt gctgggccga
    38461 cggtggaatt acgctggcag ctgcacagca actggcagca gctggggcgc aacatatggt
    38521 gattgggcgc gccctgttct cttcatctga ctatcgcgcg accttagctc aattcgccac
    38581 cctgtaagag gagattgttt ctgatgagcc aacagagacc tgaccgtatc aaacagatgc
    38641 tgcactacct gtggcaacat cgtcacctct ccacacagca ggccatggag ctttttggct
    38701 atgcggaagc gactgtccgt cgtgatttcc agtatattgt caaccagtat ccaggcatga
    38761 tccgcggtca cggttgcctg gacttcgacg acagtacaga cgacaaagag tacgtcttcg
    38821 acgtgaaacg tacattgcaa tctgtggcga aaagagaaat agctgccctt gcccgaacaa
    38881 tgatcaaaga tggcgactgc ttttttctcg actccggctc aacctgcctc gaactggcga
    38941 aatgcctggc cgatgcgcgg gtaaaggtca tctgcaacga cattaaaatc gccaatgaac
    39001 tgggctgttt tccccatgtg gaaagttaca ttatcggagg actgattcgc ccaggttatt
    39061 tttcggtagg agagagtctg gcgctggaga tgatcaatgc cttttccgta gaacgtgcgt
    39121 ttatctcctg cgatgcgctg tcgctggaga cggggatcac caatgccacc atgtttgagg
    39181 tcggggtgaa aacacggatt attcaacgct cgcgtgaagt tattctcatg gccgatcact
    39241 caaaatttga tgccgttgag ccgcatgctg tagcaacact atcatgtatt aaaacgatca
    39301 tcagtgacag cgggttacct gagactattg cacagcgtta tcagcgagcc ggatgtcagt
    39361 tgtttttacc tcattcaata aagtgataag taagttcatt cgagagggat ttcaagcaaa
    39421 aataatcaat ggcacccaat agaaaatatt ggcgatgcgc tcgaacgaat aaagaagctc
    39481 taggcgcaat ccacacacta cgatgttgca acaacacgcc atctactttt tattctcatt
    39541 cactaaatgt ggctgttctg gattcattat tcaaagtgtg tacaagatca catttaatca
    39601 catcattacg gttcagcatg ctgaacaaag catattttcc actatgtaat gccgatacca
    39661 tttattccat gagcaaggag gagccattat ggttcgcaaa gggtgtaatt cattagtccg
    39721 tgctgaaaaa atattaaccc acattgcatg ggttggtatg gcaagttata tggagttatt
    39781 aaataaattc cagtacccca aaagcagcct gcttaattta ttgaatgtaa tggtggattg
    39841 tggtttctta attaaaaaca aaaacggcta ctattcactg ggaataaaaa actacgaatt
    39901 gggttgtcag gcgttacacc gtcaaaatat atttgaggtg actaagcggc caatgcagga
    39961 gttgtcgctg aaaagtggac tggtttgtca tcttggggcg atggagagca tttcggctat
    40021 ctatctcgac aagatcgaaa gtccggactc tgtaccgacg agcaaaagct ggattggcaa
    40081 aaaacttgag ttgcatatta ccgcgttggg taaggcatta ttagcctgga agacacgaga
    40141 agaactggat tattttttag aagcactcac gttaactcca catacgcgca atacatttac
    40201 cgataaaaaa ttgtttctgg aagaattaca aaaaacacgc cttcggggat gggccataga
    40261 caacgaagaa tcaacctatg gggcggtatg tttaagtatg ccggtattca atatgtataa
    40321 cagagttaac tatgcaatct cactttctgg cgatccggta gtttattcag gaaataagat
    40381 agacagctat ctggaattgc tccggaaatg cgctgagcaa atatcatatg ggctgggata
    40441 cagaaacgaa aatgagcact tacgaaaagg aaactgaggt aatgaaaaaa ttcagcggca
    40501 ttattccacc ggtatccagc acgtttcatc gtgacggaac ccttgataaa aaggcaatgc
    40561 gcgaagttgc cgacttcctg attaataaag gggtcgacgg gctgttttat ctgggtaccg
    40621 gtggtgaatt tagccaaatg aatacagccc agcgcatggc actcgccgaa gaagctgtaa
    40681 ccattgtcga cgggcgagtg ccggtattga ttggcgtcgg ttccccttcc actgacgaag
    40741 cggtcaaact ggcgcagcat gcgcaagcct acggcgctga tggtatcgtc gccatcaacc
    40801 cctactactg gaaagtcgca ccacgaaatc ttgacgacta ttaccagcag atcgcccgta
    40861 gcgtcaccct accggtgatc ctgtacaact ttccggatct gacgggtcag gacttaaccc
    40921 cggaaaccgt gacgcgtctg gctctgcaaa acgagaatat cgttggcatc aaagacacca
    40981 tcgacagcgt tggtcacttg cgtacgatga tcaacacagt taagtcggta cgcccgtcgt
    41041 tttcggtatt ctgcggttac gatgatcatt tgctgaatac gatgctgctg ggcggcgacg
    41101 gtgcgataac cgccagcgct aactttgctc cggaactctc cgtcggcatc taccgcgcct
    41161 ggcgtgaagg cgatctggcg accgctgcga cgctgaataa aaaactacta caactgcccg
    41221 ctatttacgc cctcgaaaca ccgtttgtct cactgatcaa atacagcatg cagtgtgtcg
    41281 ggctgcctgt agagacatat tgcttaccac cgattcttga agcatctgaa gaagcaaaag
    41341 ataaagtcca cgtgctgctt accgcgcagg gcattttacc agtctgagga gaaactcatg
    41401 tctgttcgca atatttttgc tgacgagagc cacgatattt acaccgtcag aacgcacgcc
    41461 gatggcccgg acggcgaact cccattaacc gcagagatgc ttatcaaccg cccgagcggg
    41521 gatctgttcg gtatgaccat gaatgccgga atgggttggt ctccggacga gctggatcgg
    41581 gacggtattt tactgctcag tacactcggt ggcttacgcg gcgcagacgg taaacccgtg
    41641 gcgctggcgt tgcaccaggg gcattacgaa ctggacatcc agatgaaagc ggcggccgag
    41701 gttattaaag ccaaccatgc cctgccctat gccgtgtacg tctccgatcc ttgtgacggg
    41761 cgtactcagg gtacaacggg gatgtttgat tcgctaccat accgaaatga cgcatcgatg
    41821 gtaatgcgcc gccttattcg ctctctgccc gacgcgaaag cagttattgg tgtggcgagt
    41881 tgcgataagg ggcttccggc caccatgatg gcactcgccg cgcagcacaa catcgcaacc
    41941 gtgctggtcc ccggcggcgc gacgctgccc gcaaaggatg gagaagacaa cggcaaggtg
    42001 caaaccattg gcgcacgctt cgccaatggc gaattatctc tacaggacgc acgccgtgcg
    42061 ggctgtaaag cctgtgcctc ttccggcggc ggctgtcaat ttttgggcac tgccgggaca
    42121 tctcaggtgg tggccgaagg attgggactg gcaatcccac attcagccct ggccccttcc
    42181 ggtgagcctg tgtggcggga gatcgccaga gcttccgcgc gagctgcgct gaacctgagt
    42241 caaaaaggca tcaccacccg ggaaattctc accgataaag cgatagagaa tgcgatgacg
    42301 gtccatgccg cgttcggtgg ttcaacaaac ctgctgttac acatcccggc aattgctcac
    42361 caggcaggtt gccatatccc gaccgttgat gactggatcc gcatcaacaa gcgcgtgccc
    42421 cgactggtga gcgtactgcc taatggcccg gtttatcatc caacggtcaa tgcctttatg
    42481 gcaggtggtg tgccggaagt catgttgcat ctgcgcagcc tcggattgtt gcatgaagac
    42541 gttatgacgg ttaccggcag cacgctgaaa gaaaacctcg actggtggga gcactccgaa
    42601 cggcgtcagc ggttcaagca actcctgctc gatcaggaac aaatcaacgc tgacgaagtg
    42661 atcatgtctc cgcagcaagc aaaagcgcgc ggattaacct caactatcac cttcccggtg
    42721 ggcaatattg cgccagaagg ttcggtgatc aaatccaccg ccattgaccc ctcgatgatt
    42781 gatgagcaag gtatctatta ccataaaggt gtggcgaagg tttatctgtc cgagaaaagt
    42841 gcgatttacg atatcaaaca tgacaagatc aaggcgggcg atattctggt cattattggc
    42901 gttggacctt caggtacagg gatggaagaa acctaccagg ttaccagtgc cctgaagcat
    42961 ctgtcatacg gtaagcatgt ttcgttaatc accgatgcac gtttctcggg cgtttctact
    43021 ggcgcgtgca tcggccatgt ggggccagaa gcgctggccg gaggccccat cggtaaatta
    43081 cgcaccgggg atttaattga aattaaaatt gattgtcgcg agcttcacgg cgaagtcaat
    43141 ttcctcggaa cccgtagcga tgaacaatta ccttcacagg aggaggcaac tgcaatatta
    43201 aatgccagac ccagccatca ggatttactt cccgatcctg aattgccaga tgatacccgg
    43261 ctatgggcaa tgcttcaggc cgtgagtggt gggacatgga ccggttgtat ttatgatgta
    43321 aacaaaattg gcgcggcttt gcgcgatttt atgaataaaa actgaaagca taaataaaaa
    43381 catccagaca cctgaattat ccgactctgc atcattcgtt atttgctcag tcggataatg
    43441 atgggtcgct accctgttat gagaggacgt tatgccacta attatcgttg tggcagggat
    43501 tgctttactc ctgcttttaa ccataaaaat taagcttaat acgtttgttt cgttaattat
    43561 tgtctcgatt gctgtcgcca tcgccagtgg tatggatttg agtaaagtcg tcacctcagt
    43621 cgaatccgga cttggcggta cgctgggcca tattggtttg atatttggct tcggtgttat
    43681 gctcggtcgc ttgctggccg atgcgggtgg tgcgcagcga atagcgctga ccatgctgaa
    43741 ttatttcggg aaaaataaac ttgactgggc agtggtctgt tcggcgttta ttgtcggtat
    43801 cgcacttttt ttcgaagtcg gtttaattct tctggtacct attttattcg ctattgcccg
    43861 tgaagcaaaa atatcaccga tgtttatgtg tgtgccgatg ctttctggtt tgctggtcgc
    43921 acacggtttt ttgccgccgc accccggccc gactgttatc gccagggaat atggcgctga
    43981 tgtaggattg gtactgatat acggtatcat tgtcggcatt ccaaccttta ttctttgcgg
    44041 cccggtattg aataaattct gccagcgcat tattccggac gcctttaaaa aagaggggaa
    44101 tatagcctct ctcggcgcga cccgccgatt cagcgaaagc gaaatgccag gctttgggat
    44161 cagttttctc acggcaatgc tgccggtgat cctgatggcg gttgtcacca tcatccagat
    44221 gactcatgcc aaaagtgccg cggattcagg cttattttac aacgtgatac tgttcttagg
    44281 taattcaacg atcgccatgc tgatttcact gctattcgcg atctatacca tgggcctggg
    44341 gcgcgggaaa acaatccctg acctgatgga ttcttgcgga aaagctatcg cgggtattgc
    44401 cggtctgctg ctgattatcg gtggtggtgg tgcattcaag caagttctca tcgactctgg
    44461 cgttggtcag tatatttcca ccctggtttc gggtatggat attaacccga tcctgatggc
    44521 ctggggcgtg gccgcattcc tgcgtatttg tctgggttca gccaccgtcg cggcgatctc
    44581 taccgccggt ctggtcatcc cattgttggc agtacatccc aataccaacc tggccttgat
    44641 tacgctggct accggtgccg gatcctgtat ttgttctcac gttaacgatg ccagtttctg
    44701 gatgattaaa gacttttttg gcctgaccac caaagaaacg ctgttatcct ggacgctgat
    44761 gtccacactc ttatccatca gtgggctgat ttttattctg ctcgccagct tagttttata
    44821 atcgggttct tgccagtgag ccaacctgtt gttcactgtc ccccataatt tagtgagtga
    44881 cggcaaccgt ctgcacggtg acgaatcgct ggcgatcaaa aaggggcgat attgaacctt
    44941 cgattatgct tgatgggtgt attctcagta atactgtaaa gccggagcat gtctccggct
    45001 ttacagtatg ggggaacagc gggcgatcgc tcaaatggta tgctggttta tttcatgtaa
    45061 aagaataaaa tatctctata aaatcatgcc actggcaatc tgcatcaact ctccgggtaa
    45121 acagggttaa gatgagcatt tccccggttt aatacaggta tacaaaagtg gatagagatc
    45181 aaacaaacag cagcctgttc aatgatgatc ccgtacttca tgcaacatgg ctgtactatc
    45241 aggaaggtaa aagccagact gaagttgcag ctatcatggg ggtgtcccgc gtcacggttg
    45301 tcaaatacct gcaaacagcc cgggaaaatg gactggttca tatcaacctg gatgtgaatg
    45361 tctttggctc tatcgacgct gctttgcaaa ttcgcgataa attcaatctc cagagagtga
    45421 tcatcgtgcc ggatggcgaa catgccggaa aacgtgatga tacaaagctg atgcggactc
    45481 gcctttcacg tgccggtggc atgtatctga atcaggtgat tgagaatggt gatgtgctgg
    45541 gggttgcctg gggaagaaca atccatcaga tgagcaaaac catgacgccg aagtcatgta
    45601 aaaatgtcac cgttatacag atgctcggtt ccatgccatc tcagccagac ctgacgatca
    45661 ttgaatcctc atcgcaaatc gcctacaagc tttcaggccg tgtcgcctcg ctgcacgtgc
    45721 ccgccgtggt atcaagcgcc agactggcca tggagttaca ggcagagcct attattcgat
    45781 cgaattttga cgttctgact cgctgtacga aagccttttt tgtcgtgggt aacgcgcttg
    45841 atgaaaatcc cctgattcgg gtgggcgtgc tgaataagaa ggaaatgcag acgtatcggg
    45901 atttaggcgc agtaggtgtc atttgtggtc gcttctatga caaggaaggg atgcccgtcg
    45961 tggctgatgt tgaccagcgt attctgggaa tcagtctggc tcagctaagg cagattgaac
    46021 ggaagatctt ccttgctggt ggggaaagag gctatgacgc aacgttgggt gccctgctgg
    46081 gaggatatgt cacggacctg attgttgatg aaggaacggc agagtttttg ctggcgtgtg
    46141 aattaccaca ctaaaagaat gaacaactgc cccatgtaga tatggggcag gattttattg
    46201 attagcggcg ataataagaa caatacccac aatgtatgaa ataaacatgg tcagcagcag
    46261 tttattggtt ccggctggcg cactggcaaa ttctctccag atgaagactc cccagatcac
    46321 ggcaatcatt gtggccccct gtcccagacc gtaggaaatg gcataccccg cctgaccaga
    46381 cgcaatcaga ctgaatgcca gaccgacaca ccaaatcata ccgcccagcc agccgcaaac
    46441 atgatcccgt aaactcccgg aaaaatacat tttcccgttg accggttctc ctgaaatagg
    46501 ttttttcatg accagcctgt taaggacaaa gttcgacaaa aacagcccca cggcaaacag
    46561 aacaagtgct gagtaaggcg tcatcagacc gctggcaggc tggctgaaat tgtcagacat
    46621 ggagtccgcc aggaagcgga aaaaccatcc catgatgata cccgccagaa tggcagtaat
    46681 gagaccttta ttacggcgag atttatccgc ctcctgcgtg acacgaccat aggctatcgc
    46741 ggtaaaaata atggcgatca caacgcagac gacaccgagg aacagaatga gcggatcgcc
    46801 ctgcggattg ccgatatagg tggtgatcac acccagagcc agtgccagcc ccacacccac
    46861 aggaaaagct accgccatac cggcaagatt aatagaagcg accagaagaa tattggaaat
    46921 attgaagata atccctgcca gtattgccga catcagacta ctgctgctcg cctgctgaat
    46981 atcaggtata aaactgcgac cggcttcgcc catagaaccc agcgtgaagg caaataacaa
    47041 agaacacagc aataatccaa ttgaataatc ccaatagaat agagggaact cccatttttt
    47101 attgctgaca agtttagttg tattcgccca tgaaccccag caaatcatgg taataaaaca
    47161 cattattatg gcgacagcat acgattccac gataaacata aataactcct tgtggtagtt
    47221 ataatcaatc gttgtggaca gagggaatag cagccattct gcgaatggct gtcatatgtt
    47281 attaattaaa ataaagctgt aattacgctt tcaaaccata cgttttgaat ttttcaagga
    47341 cgatggtcat ttcggcttca gacagcacgg gaacatcgtg atgtaatccg ccacatttga
    47401 tgtataaatc agccagcgtc tcggtttcac ccgccagcca caacgtcttt tccagcgtga
    47461 cgtccatggc cagcataccg tggtgctgca tcagcaagga cttactttct ctgattcctt
    47521 tagatacacc gtcggccagt tcaggactgc caaaggtata atagggaata cagggaatat
    47581 gatccgtccc cgataccgcc accatataat gaatggccgg aattgaatga ttatgtattg
    47641 ccaccgccgt ggaattcacc gcatgcgtat gtatgacagc attcaattct ggtctggctt
    47701 tataacacgc cagatgaatt aaccattcac ttgatggtat tttacccgct tcaggttttc
    47761 ccttatcgtc tacaaagaca atcatgtccg gcgtcatctt tgaataagcg atcccactcg
    47821 gcgtaatcaa catgccaccg gtatacctgg cactcacatt acctgatgtt ccttgattaa
    47881 gacctgacgc attcatttcc cgacaagtgg aaatgatttt ttctgctaac ttaatccgtt
    47941 ccattcctga tccttaatag caatggccgc tgttttaatg ctgttccccc tggagtgctt
    48001 tactcttcag gtcagcaaac atatccgcca gcgctgactt actggcagca cagactcccc
    48061 gctcagtaat gaagccggta atgtatcggg caggcgtgac gtcgaaagcg tagttcccac
    48121 aacgggttcc ttcaggggcg gtattgaccc agctcaattc tccctgaggg tttatcccat
    48181 agacatggga ctgctctttt ccgtcacgtt gctcaatcgg aatgcttttt ccgtcctcga
    48241 tagtccagtc aatagtggga gaaggtaacg cgacgtagaa gggaacatgg ttatcatggg
    48301 ccgccagcgc cttaagatag gtaccaattt tattacagac atcccctctg gccgtggttc
    48361 gatcggtccc aacaatgcag agatcaacgt caccgtgttg catcaggtgg ccacccgcgt
    48421 tgtcggcgat cagggtgtga ggaataccgt gtgagcccag ttcaaatgcc gtgagtccgc
    48481 cctgattacg tggccgcgtt tcatccaccc agacgtgaac agggattccg ttttcatgcg
    48541 ctttgtagat gggagaaagc gcggttcccc agtctacagt cgccagccag cctgcgttac
    48601 aatgtgtcag gatattgacc acactcccgg ccggtttttt ctgggctatt tcgcggatga
    48661 cttctgcacc atgtaatcca atttgttcac acaacgcgac atcttcctcg gcaatttctg
    48721 ccgccagcgc ccaggccaca tccttgcgct ctgatacaca gagatccttc agtgtgtcta
    48781 tcatcctgtc gagggcccat ttgagattaa ttgccgttgg tcgggttttg ataagaaggt
    48841 catagtaacg ctgcagaccc atgtcactgg catcgtgatt cattcccagc gcgatgccat
    48901 aggctgcaac gacaccaata agcggagcgc cacgaaccca catatcctga atagccgtag
    48961 cggccatttc cgcagaggta agcgccacca cctcgaactt aaaaggcaac ttagtctgat
    49021 cgatgatttc taccgctttt ccatccccgg aaacccagac cgtacgatag tgtttacctt
    49081 taatattcat aatgacctct tacttaacct tcgcagactg gatgacggaa tccatatttt
    49141 taaactggcg ggcattgacg ataagatcgc gcgccatggt caatgcacgt cgttcacatc
    49201 ctgcgcgaag ctctgtattt tcaatctgtt tcagctcggc aacgccagca aaaccaatga
    49261 tccggcgatt catttccatg ccggcattca ccagggaatc ttcaagcagc gtggcaaaaa
    49321 actgatcctg tgcgtcctca agagcggccc tgttctgttg atacatttct gtcgaccacg
    49381 cgtcgccctg tgttttgttg tcccagagct ggcggaactc ccgggtgaaa acggaccagg
    49441 tttggacaat ctgctgaagc agccattcct gatagtcagc gcgacgttgc tcattcgcat
    49501 cccacccagg gcgtgagaag tacgccagca ggaggttgcc aatatagttg ccgatatcaa
    49561 acgccattgg ccccatgaaa ccgaactccg gatcaatcac tttggtatcc gtgtcggtca
    49621 ccatgattga gcctgaatga aggtcgccat gtaacaatgc ctgcgcttct gtcataaatt
    49681 tgtacttata acgcatggca acctggatca tctctacatc agcccaggcc ttatggacgg
    49741 catcgtccag ctcaggagaa gtccagttat tccgttcagc gttgtaatag ggctctgtga
    49801 agatcagatc ttccgtaatt ttgcacagct catgattcaa cgcgaactcg gcagtaagcg
    49861 cttttttctg ttctgctgac atgccaatgt cagacgtatt gaagagagtc tgtgctaaaa
    49921 atctgccgat atcttcagct agtttaggga attttttacc gttaatcagt tccttacgca
    49981 gaatcacgtg aggagtcaga tactccatgg caaacagcgc catttccggg tcgtaaaaat
    50041 agacctgcgg aatcagtgtg tgacccgcgt acttggcttc ctgacataag acgttgtact
    50101 caaagcccgc acggctcaga gataaaggcc atccttcgcc cccggcacga agccagggca
    50161 gagcttgttt tacaatgatg gttttatgtg tcccctcaac gataaacacc atgttcaggt
    50221 ttccgtcccc cacttctgaa actttccact cttcaggtaa ccctcccggt tcgcatgaag
    50281 gggtcagtct ggacgagaga taacccggca gcgtgtcaca tgtcagcggc ttgtaacctg
    50341 atggaattga atccgtcata acagttctcc ttgtctacac agaatgataa tcaacttgtc
    50401 atgtgacaac tatacattat caaatgtaaa attatttgtg acacatgtag attttttgaa
    50461 ctgtataaaa ttaaaaatga tgtcgtatgg agtcatgatc cttacaggaa tcagcagagg
    50521 aaaacatcta ttattaccat gtaattcatg ttgttatgtc gcaacaatag tactgcacgg
    50581 ggaattatta cgggcaaact taggcgaaag gaagtactga agtgacagaa taaatcacac
    50641 tttttgtaca catgtacttt tctattgtct tttgttgtgg aaacaaatgg tgtttgtcat
    50701 gccgatgcta atagacagat agattcgagg gccaacagcc ccatcatcat gatggagcaa
    50761 cagaggtgag tctaacgttt ccgcacttct tatcgatcgg cactaacgat caatatcgat
    50821 aaactgatct atcaaaacga ttatctttct tgcaacctgc ctagttatcc ttctctcaca
    50881 ttgctcgcag aaaagccctt ttattacggt tagttatcta gctgaggctt gctacgccca
    50941 ttttccatgt accccaaatc acgaggtcac aaaggcgcgg aaagatgaag ggatgtgcac
    51001 gttgcaacaa aaaaagaagc gtccttgaac agaggctctc actcgataaa gaattctgcg
    51061 acgagtgctt attcgatatc gatgaggaaa tcagggaatg ctggtccagt aaccgggtga
    51121 gcagtgtctg ccagatggtg actggagacg ggcagcacga tacctcccgt acatgcgtgt
    51181 cgggtaatgt tttcatactg aagattttcc tgatatgcag agataaaaaa tgggacagtg
    51241 gcgtggtgaa aacaccaggc cggagcagaa ggttattctg gtggtttact ttttcgtttc
    51301 aggtgtcgga taaacagcca gataaacgta accacaggag ccgagggtgt cggcttcaca
    51361 ggtcagcccc ttcgcataca gtgtcacggt atgctgatgg cggggattca gttcaccact
    51421 ggtgagcatt aatttcagtt gtttcatcag cagcggaaag gcctggtcca gagggtacgc
    51481 atccgcatct ctgaaccggc ctctgatacc ggcacggtcg gcaaggtaat gcaaccggtt
    51541 accctcctgc accagacggg cgccgaaaca gggcgtcact gtgcagggta gtccccacca
    51601 gggatggtcg tcattgtcat cgggatgcgt tgtcccggaa agtgtgtctg acacgataaa
    51661 atcctcacag aaaatcggtg aatgatagtt taacgatgac gggtcagccg gcgcgcagac
    51721 cggcttcggt aaaggtgtat tcgttgatga gcaggacctc atccacagcg gcgtctgagc
    51781 tctgccagtc gtactcgttt tccgggtgac ggaacatcag ttcagggtga cgctcatcag
    51841 ccggacatac ggaccaaaac tgtccttacg gcgttctgca aacacggcca gcacaccggg
    51901 aatatcctgc acttcacgac cggtatacgc ttcagcactg ccgtgccagc ggtatttgcc
    51961 ggtacagaac ggaaaaagac gggatgccgg atgctgacgg tgaatacgca tggcttcgcc
    52021 acgggtgatg attttcatag tgggatacct ctgaagacag aagataaaag tgaaaacagg
    52081 tgtgatgtgg ttgtgacggg ttaaagcaga ccatattc
//
LOCUS       DAPOZO010000026        50367 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPOZO010000026 DAPOZO010000000
VERSION     DAPOZO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.026"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(54..800)
                     /gene="cutC"
                     /locus_tag="ROI21_001652"
     CDS             complement(54..800)
                     /gene="cutC"
                     /locus_tag="ROI21_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDX5110583.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGVARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLNAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERRQAK"
     gene            complement(814..1380)
                     /locus_tag="ROI21_001653"
     CDS             complement(814..1380)
                     /locus_tag="ROI21_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDX5110584.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1596..3329
                     /gene="argS"
                     /locus_tag="ROI21_001654"
     CDS             1596..3329
                     /gene="argS"
                     /locus_tag="ROI21_001654"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDX5110585.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3382..3774)
                     /gene="flhe"
                     /locus_tag="ROI21_001655"
     CDS             complement(3382..3774)
                     /gene="flhe"
                     /locus_tag="ROI21_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDX5110586.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAIEPL
                     RFIWEVPGDGRLIPPLKVQRNEVIVNYR"
     gene            complement(3774..5852)
                     /gene="flhA"
                     /locus_tag="ROI21_001656"
     CDS             complement(3774..5852)
                     /gene="flhA"
                     /locus_tag="ROI21_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDX5110587.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGTGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAS
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(5845..6993)
                     /gene="flhB"
                     /locus_tag="ROI21_001657"
     CDS             complement(5845..6993)
                     /gene="flhB"
                     /locus_tag="ROI21_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDX5110588.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7195..7839)
                     /gene="cheZ"
                     /locus_tag="ROI21_001658"
     CDS             complement(7195..7839)
                     /gene="cheZ"
                     /locus_tag="ROI21_001658"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDX5110589.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(7850..8239)
                     /gene="cheY"
                     /locus_tag="ROI21_001659"
     CDS             complement(7850..8239)
                     /gene="cheY"
                     /locus_tag="ROI21_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDX5110590.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8254..9303)
                     /gene="cheB"
                     /locus_tag="ROI21_001660"
     CDS             complement(8254..9303)
                     /gene="cheB"
                     /locus_tag="ROI21_001660"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDX5110591.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9306..10166)
                     /gene="cheR"
                     /locus_tag="ROI21_001661"
     CDS             complement(9306..10166)
                     /gene="cheR"
                     /locus_tag="ROI21_001661"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDX5110592.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10185..11786)
                     /gene="tap"
                     /locus_tag="ROI21_001662"
     CDS             complement(10185..11786)
                     /gene="tap"
                     /locus_tag="ROI21_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDX5110593.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAAATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLHIAPVVS"
     gene            complement(11832..13493)
                     /gene="tar"
                     /locus_tag="ROI21_001663"
     CDS             complement(11832..13493)
                     /gene="tar"
                     /locus_tag="ROI21_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDX5110594.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIASGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(13638..14141)
                     /gene="cheW"
                     /locus_tag="ROI21_001664"
     CDS             complement(13638..14141)
                     /gene="cheW"
                     /locus_tag="ROI21_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDX5110595.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14162..16126)
                     /gene="cheA"
                     /locus_tag="ROI21_001665"
     CDS             complement(14162..16126)
                     /gene="cheA"
                     /locus_tag="ROI21_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDX5110596.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKDETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGTDSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETIEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16131..17057)
                     /gene="motB"
                     /locus_tag="ROI21_001666"
     CDS             complement(16131..17057)
                     /gene="motB"
                     /locus_tag="ROI21_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDX5110597.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEKSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEEAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17054..17941)
                     /gene="motA"
                     /locus_tag="ROI21_001667"
     CDS             complement(17054..17941)
                     /gene="motA"
                     /locus_tag="ROI21_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDX5110598.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18068..18646)
                     /gene="flhC"
                     /locus_tag="ROI21_001668"
     CDS             complement(18068..18646)
                     /gene="flhC"
                     /locus_tag="ROI21_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDX5110599.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEDGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(18649..18999)
                     /gene="flhD"
                     /locus_tag="ROI21_001669"
     CDS             complement(18649..18999)
                     /gene="flhD"
                     /locus_tag="ROI21_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDX5110600.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            19780..20208
                     /gene="uspC"
                     /locus_tag="ROI21_001670"
     CDS             19780..20208
                     /gene="uspC"
                     /locus_tag="ROI21_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDX5110601.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(20215..21639)
                     /gene="otsA"
                     /locus_tag="ROI21_001671"
     CDS             complement(20215..21639)
                     /gene="otsA"
                     /locus_tag="ROI21_001671"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDX5110602.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKIA"
     gene            complement(21614..22414)
                     /gene="otsB"
                     /locus_tag="ROI21_001672"
     CDS             complement(21614..22414)
                     /gene="otsB"
                     /locus_tag="ROI21_001672"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDX5110603.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLAIASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(22581..23567)
                     /gene="araH"
                     /locus_tag="ROI21_001673"
     CDS             complement(22581..23567)
                     /gene="araH"
                     /locus_tag="ROI21_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDX5110604.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(23582..25096)
                     /gene="araG"
                     /locus_tag="ROI21_001674"
     CDS             complement(23582..25096)
                     /gene="araG"
                     /locus_tag="ROI21_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDX5110605.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(25166..26155)
                     /gene="araF"
                     /locus_tag="ROI21_001675"
     CDS             complement(25166..26155)
                     /gene="araF"
                     /locus_tag="ROI21_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDX5110606.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            26951..27454
                     /locus_tag="ROI21_001676"
     CDS             26951..27454
                     /locus_tag="ROI21_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDX5110607.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(27534..27785)
                     /locus_tag="ROI21_001677"
     CDS             complement(27534..27785)
                     /locus_tag="ROI21_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDX5110608.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(27900..27986)
                     /gene="azuC"
                     /locus_tag="ROI21_001678"
     CDS             complement(27900..27986)
                     /gene="azuC"
                     /locus_tag="ROI21_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDX5110609.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            28248..28571
                     /locus_tag="ROI21_001679"
     CDS             28248..28571
                     /locus_tag="ROI21_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDX5110610.1"
                     /translation="MRLLILTLSLITLVGCTVTRQAHVGEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            28742..29239
                     /gene="ftnA"
                     /locus_tag="ROI21_001680"
     CDS             28742..29239
                     /gene="ftnA"
                     /locus_tag="ROI21_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDX5110611.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(29277..29516)
                     /locus_tag="ROI21_001681"
     CDS             complement(29277..29516)
                     /locus_tag="ROI21_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDX5110612.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLTAKGKFIPSEEGFSTDQSKICRH"
     gene            29708..30919
                     /gene="tyrP"
                     /locus_tag="ROI21_001682"
     CDS             29708..30919
                     /gene="tyrP"
                     /locus_tag="ROI21_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDX5110613.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMSLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(30969..31634)
                     /gene="yecA"
                     /locus_tag="ROI21_001683"
     CDS             complement(30969..31634)
                     /gene="yecA"
                     /locus_tag="ROI21_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDX5110614.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERLEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(31830..31916)
                     /locus_tag="ROI21_001684"
     tRNA            complement(31830..31916)
                     /locus_tag="ROI21_001684"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31880..31882),aa:Leu,seq:taa)
     gene            complement(31929..32002)
                     /locus_tag="ROI21_001685"
     tRNA            complement(31929..32002)
                     /locus_tag="ROI21_001685"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31968..31970),aa:Cys,seq:gca)
     gene            complement(32057..32132)
                     /locus_tag="ROI21_001686"
     tRNA            complement(32057..32132)
                     /locus_tag="ROI21_001686"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32097..32099),aa:Gly,seq:gcc)
     gene            complement(32284..32832)
                     /gene="pgsA"
                     /locus_tag="ROI21_001687"
     CDS             complement(32284..32832)
                     /gene="pgsA"
                     /locus_tag="ROI21_001687"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDX5110615.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(32889..34721)
                     /gene="uvrC"
                     /locus_tag="ROI21_001688"
     CDS             complement(32889..34721)
                     /gene="uvrC"
                     /locus_tag="ROI21_001688"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDX5110616.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(34718..35374)
                     /gene="uvrY"
                     /locus_tag="ROI21_001689"
     CDS             complement(34718..35374)
                     /gene="uvrY"
                     /locus_tag="ROI21_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDX5110617.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            35670..35846
                     /locus_tag="ROI21_001690"
     CDS             35670..35846
                     /locus_tag="ROI21_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110618.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            35833..36057
                     /locus_tag="ROI21_001691"
     CDS             35833..36057
                     /locus_tag="ROI21_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDX5110619.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(36124..36846)
                     /gene="sdiA"
                     /locus_tag="ROI21_001692"
     CDS             complement(36124..36846)
                     /gene="sdiA"
                     /locus_tag="ROI21_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDX5110620.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(37076..37828)
                     /gene="yecC"
                     /locus_tag="ROI21_001693"
     CDS             complement(37076..37828)
                     /gene="yecC"
                     /locus_tag="ROI21_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDX5110621.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(37825..38493)
                     /gene="tcyL"
                     /locus_tag="ROI21_001694"
     CDS             complement(37825..38493)
                     /gene="tcyL"
                     /locus_tag="ROI21_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDX5110622.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(38508..39494)
                     /gene="dcyD"
                     /locus_tag="ROI21_001695"
     CDS             complement(38508..39494)
                     /gene="dcyD"
                     /locus_tag="ROI21_001695"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDX5110623.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVHISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(39599..40399)
                     /gene="tcyJ"
                     /locus_tag="ROI21_001696"
     CDS             complement(39599..40399)
                     /gene="tcyJ"
                     /locus_tag="ROI21_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX5110624.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(40487..41038)
                     /gene="fliZ"
                     /locus_tag="ROI21_001697"
     CDS             complement(40487..41038)
                     /gene="fliZ"
                     /locus_tag="ROI21_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDX5110625.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(41084..41803)
                     /gene="fliA"
                     /locus_tag="ROI21_001698"
     CDS             complement(41084..41803)
                     /gene="fliA"
                     /locus_tag="ROI21_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDX5110626.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATEPEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(41967..43619)
                     /locus_tag="ROI21_001699"
     CDS             complement(41967..43619)
                     /locus_tag="ROI21_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HDX5110627.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSTSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGETANTAATLKDMSGFTAAAAPGGTVGVTQYTDKSAVASS
                     VDILNAVAGTDGNKVTTSADVGFGTPAAAVTYTYNKDTNSYSAASDDISSANLAAFLN
                     PQAGDTTKATVTIGGKDQDVNIDKSGNLTAADDGAVLYMDATGNLTKNNAGGDTQATL
                     AKLATATATGAKAATIQTDKGTFTSDGTTFDGASMSIDANTFANAVKNDTYTATVGAK
                     TYSVTTGSAAADTAYMSNGVLSDTPPTYYAQADGSITTTEDAAAGKLVYKGSDGKLTT
                     DTTSKAESTSDPLAALDDAISQIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            43885..45291
                     /gene="fliD"
                     /locus_tag="ROI21_001700"
     CDS             43885..45291
                     /gene="fliD"
                     /locus_tag="ROI21_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDX5110628.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASNNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            45316..45726
                     /gene="fliS"
                     /locus_tag="ROI21_001701"
     CDS             45316..45726
                     /gene="fliS"
                     /locus_tag="ROI21_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDX5110629.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            45726..46091
                     /gene="fliT"
                     /locus_tag="ROI21_001702"
     CDS             45726..46091
                     /gene="fliT"
                     /locus_tag="ROI21_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDX5110630.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            46169..47656
                     /gene="amyA"
                     /locus_tag="ROI21_001703"
     CDS             46169..47656
                     /gene="amyA"
                     /locus_tag="ROI21_001703"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDX5110631.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(47690..48103)
                     /locus_tag="ROI21_001704"
     CDS             complement(47690..48103)
                     /locus_tag="ROI21_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX5110632.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            48290..49495
                     /gene="yedE"
                     /locus_tag="ROI21_001705"
     CDS             48290..49495
                     /gene="yedE"
                     /locus_tag="ROI21_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDX5110633.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGLLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            49492..49725
                     /gene="yedF"
                     /locus_tag="ROI21_001706"
     CDS             49492..49725
                     /gene="yedF"
                     /locus_tag="ROI21_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDX5110634.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            49834..50331
                     /locus_tag="ROI21_001707"
     CDS             49834..50331
                     /locus_tag="ROI21_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDX5110635.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSHMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQAVFIAAIGSTPFERGDEAGRSFCKPAQGLSVTVAMA
                     VRANL"
BASE COUNT        12328 a        13300 c        12143 g        12596 t
ORIGIN      
        1 ggggcctggt acgagcaagc atcatattgg gcgacatgat gcaacggtaa aaatcatttg
       61 gcctgacggc gttcaatgat tcctttcatt tcagcaaccg ccgccccgtc tacgacataa
      121 cgcgaatact catccgcgtg tgcatctgat gacatggaca atccttgatt acgataacgc
      181 attggtgagg cttgccacgc tcccgcggag ctatggactt ccagcactcc ggcattgagg
      241 aagtggtgca ggttttctgc acggactcct gctccggcca taatgattgg agcatcacga
      301 tgggcaataa gttccataat ttttgataaa ccttgcagtg cgtctgattt ttgccctgat
      361 gtcagtaccc gagcaacgcc caattccgca agattattga gtgtatttaa agggttagcg
      421 cacatatcga aggcgcgatg aaaagtcact gccagcggac cggcagcagc cattattttt
      481 tccattcgtg gcatatcgac attcccgtca acatcgagaa cgcccgtcac cagtccagga
      541 aaacctaatt cgcggactgt gcgcacatcc tcaagaatgg cggcaaattc accgtcgctg
      601 tagcaaaaat caccaccgcg tgggcgaata ataggatgca cagggatcgt cacccgctgg
      661 cgtacggatt tcagtacccc caacgacggc gttaagcccc cctcttttgg ggctgcgcat
      721 aattcaactc tgtctgcgcc gttttgctgc gccgttagcg cgcattccat gctgtaacag
      781 caaatttcca gtaatgccat ttttgctcct taattacgcc gactgctcgc tggcaacgat
      841 ctcttcaata gaccacggat gaaatttaat ggtcgttttg ccatcggtga ccgccagcgt
      901 cgggttcggt aatcgctcat gctcaccttt tggcgaactg gttttaactg agatccccgg
      961 caggctgagt ccttcatcag aaagcaatgc cagcgcacgg gcattcagcg tttctggatc
     1021 acccggcaga acgatttcaa tatgttccca accttcgtgc gggtaacgtt tttccccagg
     1081 ccacggtagc tccacaatgg aaaactgcca gtgagcaacc tgtaccggtt catgcaattt
     1141 aaacagacaa atcggcctgc cattgatcat attttctgac aaaagctcgc cgcactgttc
     1201 aaacccgcga cgccagcgtt cagcggtgac gttttgatgg caacgcaagg aaatgtgatc
     1261 ggcagtcagc ggagtgatat ccaaccccag acggcgggaa agttcatcta atgcgtgggt
     1321 aaatcgcggt aaatccgatg caatatcctg cagctcgtcg atagattgcc agttcgccat
     1381 aatcactctt cgtctttcag taaaagcgtt aatttaccct gttgccctgc gccaaccaac
     1441 cgctgatttc acgccgcttc tgatgcaata gtgaaaacgg caatacgcca cgcgcacgtt
     1501 gctgacgaaa acagccattt gcagtatact cccgccctaa tttctttaac tggtgcgggc
     1561 aatttttgct cgcttcatca atgtaaggta ttccggtgaa tattcaggct cttctctcag
     1621 aaaaagtccg tcaggccatg attgcggcag gcgcgcctgc ggattgcgaa ccgcaggttc
     1681 gtcagtcagc aaaagttcag ttcggcgact atcaggctaa cggcatgatg gcagttgcta
     1741 aaaaactggg tatggcaccg cgacaattag cagagcaggt gctgactcat ctggatctta
     1801 acggtatcgc cagcaaagtt gagatcgcgg gtcctggctt tatcaacatt ttccttgatc
     1861 cggcattcct ggctgaacat gttcagcagg cgctggcgtc cgatcgtctc ggtgttgcta
     1921 cgccagaaaa acagaccatt gtggttgact actctgcgcc aaacgtggcg aaagagatgc
     1981 atgtcggtca cctgcgctct accattattg gtgacgcagc agttcgtact ctggagttcc
     2041 tcggtcacaa agtgattcgc gcaaaccacg tcggcgactg gggtactcag ttcggtatgc
     2101 tgattgcgtg gttggaaaag cagcagcaag aaaacgccgg tgaaatggag ctggctgacc
     2161 ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga agatgaagag tttgccgagc
     2221 gtgcacgtaa ctacgtagtc aaactgcaaa gcggtgacga atatttccgc gagatgtggc
     2281 gcaaactggt cgacatcacc atgacgcaga accagatcac ctacgatcgt ctgaacgtga
     2341 cgttgacccg cgatgacgtc atgggtgaaa gtctctacaa cccaatgctg ccgggtatcg
     2401 tggcggacct gaaagccaaa ggtctggcag tagaaagtga aggcgcgacc gtcgtattcc
     2461 ttgatgagtt caaaaacaag gaaggcgaac cgatgggcgt gatcattcag aagaaagatg
     2521 gtggctatct ctacaccacc actgatatcg cctgtgcgaa atatcgttat gaaacactgc
     2581 atgccgatcg cgtgctgtat tacatcgact cccgtcagca tcaacacctg atgcaggcgt
     2641 gggctatcgt ccgtaaagca ggctatgtac cggaatccgt accgctggaa caccacatgt
     2701 tcggcatgat gctgggcaaa gacggcaaac cgttcaaaac ccgcgcgggt ggtacagtga
     2761 aactggccga tctgctggat gaagccctgg agcgtgcacg tcgtctggtg gcagaaaaga
     2821 acccggatat gccagccgac gagctggaaa aactggctaa cgcagttggt attggtgcgg
     2881 tgaaatatgc ggatctctcc aaaaaccgca ccacggacta catcttcgac tgggacaaca
     2941 tgctggcgtt tgagggtaat accgcgccat acatgcagta tgcatacacg cgtgtattgt
     3001 ccgtgttccg taaagcagaa attgacgaag agcaactggc tgcagctccg gttatcatcc
     3061 gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca gtttgaagaa accctcaccg
     3121 tggttgcccg tgaaggcacg ccgcatgtga tgtgtgctta cctgtacgat ctggctggtc
     3181 tgttctctgg cttctacgag cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta
     3241 acagccgtct gaaactggca caactgacgg cgaagacgct gaaactgggt ctggatacgc
     3301 tgggtattga gacagtagag cgtatgtaat cgcgttatac ggcaaaagcc gatgtcatgg
     3361 cagacatcgg cttttttatc attaacgata attcacaatc acttcattcc gttgtacctt
     3421 tagcggcgga attaaccgcc catcgccagg tacttcccag ataaatcgca acggttctat
     3481 cgccggtata ccggaaaagg ccacggtggt tccactttgc ccttctaatt cgacacaacg
     3541 agattgcgaa cacaagcgaa cccgcagtcc tgcaggtgtc gggccgataa gctgataacg
     3601 ccacgccacc agcgtcatca atcctgcagc aggttgtcga gtagaaagag gcgctgacga
     3661 catcgactca ccacggtgat tcagcgtaac gcccacacta ctcgcctgcc acatcccctc
     3721 cccggcggct tgcaccagca gcggaaacaa taatattgct aataaggttc tcatcattta
     3781 ccgccaattg tcgccgtcat gcggatatgt cggttatcag atagttccag attcgacagc
     3841 accactaact gcggcaggct gcggcgcagg aaacgagaca ataatggtcg cagcgcgtgg
     3901 ttcaccaaca atacaggcgg cgcacccaac atctcctgac gggatagcgc ttcctgagtt
     3961 tgcgccagca aacgatccgc cagccctggc tccagtccgc ccccaccctg caacgcctgt
     4021 agtaacaaac gttccagcgg cgtatcaagg ccaataacat ggacttcatc tttgccagga
     4081 aaccactgct gggtaatcgc ccgtcccaac gccacgcgca cgacggcggt taattcatgt
     4141 ggatcgcttt ggatggacgc atgttccgcc agcgtttcga gaatggtgcg catatcgcga
     4201 atcggtactt tttcatcgag gagattttgc agcactttat gcagtgtggt gagcgtgacg
     4261 acgccaggaa cgagatcttc cgtcagcttt ggcatctcct gggcgacgcg atccaacagc
     4321 tgttgcgcct cctgacgacc aaacagctct gcggcatgct ggctaatgag gtggttaaga
     4381 tgcgttgcca ccaccgtgct ggcctcaacc acggtgtacc cctgaatctg cgcctgttct
     4441 ttaagcgcac tttcaatcca gatagcattc aggccaaatg ccggatcgac ggtcgcctca
     4501 ccaggtaacg tcccggcagc ggtacctggg ttaattgcca gccagcgccc cggataggca
     4561 tcgccagtgc caatctctac gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc
     4621 atattgtcac gaatgtgcac caccggcggc agaaacccca tctcctgggc aaatttcttg
     4681 cggatactgc gtatacggcc caacaactca ccatcctgct ggaaatcgac catcgggatc
     4741 agtcgataac ccacttccat tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct
     4801 tcgacaacgg tattattctc tgccattttt actggttttg gttcggcagg cgctttttgt
     4861 tcacgtccgc ggatccacca ggccagtccc agcaatccgg cagtgaacag caaaaatacc
     4921 aggttcggca ttccaggcac caggccgagt aaaccgagca cggcggcgct taacaacata
     4981 acgcttgggt tactgaaaag ctgattcacc atctgctcgc caacatcctg atcggtgctg
     5041 acacgcgtaa cgatgacccc cgcggcggta gaaatcacca acgccggaat ttgtgccacc
     5101 aaaccgtcgc caatggtcaa tagcgtataa ctttccgccg cgtgtcccat gctcatgcca
     5161 tgttgcagca cgccgaccag caacccgccg acaacgttaa tgaccatgat gaggatcccg
     5221 gcgatggcat cgccgcggac aaacttactt gccccgtcca ttgagccgta aaaatcggct
     5281 tcctgagtca cttcggagcg gcgttttttc gcctcatctt caccaatcaa tccggcgtta
     5341 aggtcggcgt caatcgccat ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc
     5401 acttctgcga tacgcccggc acctttggtg ataaccataa agttgatgat cacgagaatg
     5461 acgaacacca cgataccgat agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg
     5521 accacctttc cagccgccgc cgcgccggta tgcccttcca tcaaaatgat acgggttgaa
     5581 gccacattaa gtgccagacg caacaacgtg gtaaacaaca gaatcgtcgg aaacgcagca
     5641 aactcaagcg tgcgctgggt aaacatcgcc accagcaaca ccatgatcga caaggcgata
     5701 ttaaaggtaa acagcaggtc gagaatgaat gcaggcaatg gcagcaccat catcgacaaa
     5761 atcagcaaga tcaaaatcgg tccggcaagg atctgccatt gtgtcgattt caggtttgca
     5821 ggcaggcgca gcatcgcggc cagattactc atgggtcggt ttctcgttaa taaaatccag
     5881 ggcttccggc accggaagat gagtaggttg tacagggcgc tgtccaccag ccagacgcca
     5941 gcgtttcagt tgccagaccc aggccagcac ttccgccact gcggcgtaca gttgacccgg
     6001 gatttgttga ccgatctccg catgtcgata cagcgctcgc gccagcggtg gcgcttcaag
     6061 cgtcgggacg ttattttcag cgccaatttc acgaatgcgc agcgcgacca gccctgcacc
     6121 tttagcgacc actttcggtg cgctcatttt gttttcgtca tactgcaacg ctaccgaata
     6181 gtgggtcggg ttattaacaa tgacatccgc tttcggcaca tcggccatca tccgacgccg
     6241 tgcagccgct cgctgcatct gacgtatccg ccctttaaca tgggggtcgc cttcgctttg
     6301 tttgaactca tcacgaatat cctgccgcga catacgcagc tttttcaggt ggctgaagat
     6361 ttggaaaaag acgtcaaatc ccaccattgg aatgacacca agcaccacca gcagtgcgca
     6421 tagccctacc agatccatcg cattacccat ggcggtaatc ggagactcgg ccattaagcg
     6481 catcatctgc ggccagtgat gccagagaaa aaatcccgtc acgctgccaa ccaggatggt
     6541 tttcaaaatt gctttaagca actccgcgcc agtctgtgcc gagaacatcc gtttaatccc
     6601 cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc
     6661 tcccagcatg accggagaaa tgagcgccac cagcaccacg ccgctaatca gcggcagcag
     6721 cgccagcatg gcttctctga tcagcagaat aatttgcccg aggatcagat tcgggtcatt
     6781 gataatactg tgatcaaaat gcaatcccgc ggagagcatg cccgacaatc gacgggccag
     6841 cgacacaccg ccaaaccaga taacacacac gcccactaac aaaatcagca gtgacgttaa
     6901 ttcacgtgaa cgcgggattt gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg
     6961 ggcttctgtt ttgtcgtcgc tctcgtcaga cacgtcgcca atcctggaaa gagtattaag
     7021 cgtaaatgat gccagagcgc aaagcgttca atggtttgag taaggggcaa aacaggcggg
     7081 atttagggct tttgctgcca cacatcaagc atagtatgcg tttgtcggat gcggcgtcat
     7141 cgcgttatcc gaccgcctga tatgacgtag tcacgccaca tcaggcaata caaatcaaaa
     7201 tccaagacta tccaacaaat cgtccacctg atcctgactg gctaccacac cggctttgct
     7261 ggtatcgacc tgaggtccat taagcaaact ctggttttca cgttttggac gcgactcctg
     7321 ttccgggatg ttttccaaca gcaccatcaa caactggcgt tcgatctcct gaatgacatc
     7381 catcatccgc ttaataacct gtccggtgag atcctgaaaa tcctgcgcca tcatgatttc
     7441 cagcagttgc gcgtttgtaa agctggtatg cgcgggtaca tctgccagaa attgccgcgt
     7501 gtctgttacc agttcacggg cgtcggcaag atcaatcgga tcggcaaacc agtcatccca
     7561 acgttgggtt aacgcttttg ctgatttctc catttgatcc tgatgcggtt gtgacgcctc
     7621 aacactgttc agcgcccgct ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg
     7681 atcgcgcgca tcggggatgg cttccgccgc ttcagcaatg gcctgatcca gccccagctc
     7741 ccgcaaactg tcgcgcagca tacgcgtcag gctgccgatg cgcgcaatga tatcgccagc
     7801 tgaatgctcg tcagcaggtt tgattgatgg ttgcatcata gtcgcatcct cacatgccca
     7861 gtttctcaaa gattttgttg agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca
     7921 catagccact ggcccccgct tgcgccgcag caatgatgtt ctctttcttc gcttctgcag
     7981 tcaccattaa cactggcaat gccgacatcg cgccatctgc acgaatcgtt ttcagcaact
     8041 ccaggccatc catattgggc atgttccagt cggagataac aaatccataa ccgcctgcct
     8101 gcaacttatt gagagcgtcg acaccatctt ccgcttcctc aacattattg aatcccagct
     8161 ctttcagcag gttacgcact atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt
     8221 taagttcttt atccgccatt tcacactcct gatttaaata cgtatcgcct gtccggcact
     8281 aatttttgcc agcatttgct ggcttacctg gctaagatcg accacttcgc aaacaccacc
     8341 catattgatg gcctcgcgcg gcatgccgaa caccacgcaa cttgcttcgt tttgtgcaag
     8401 ggtccatgcc cccgcctgac gcatcgccaa cattcccgcc gcaccgtcgt tgcccatacc
     8461 ggtcaggatc accccaaccg cattacgccc tgcctgtttg gcgacagaat ggaacaacac
     8521 atctaccgaa ggccgatgac ggttaaccgc cgggccgtcg tgaattttga tttgataatt
     8581 tgcaccacta cgcgccagct ccatatgccg atcgcccggc gcaatatagg catgccccgg
     8641 taagacacgt tctccgtctt cggcttcttt aaccccgatc tggcaaagct tattaagtct
     8701 gtcggcaaaa gagcgggtga aaccgggcgg catatgctgg gtaattaaca gtgccgggct
     8761 ggaaagcggc aacggttgca gtacgtgacg aattgcctca gttccacccg ttgaagcacc
     8821 aatcgcaatc agtttttcag aactcaacaa cggccccgct ttcagcgttg tcggtgccga
     8881 caatggttta tgtgccgcaa ggctcgcttt tgctgccgta cgcacctttt cagcaatcat
     8941 ttcgctatac gccagcattc cttcgcgaat acccagttgc ggtttggtga caaaatctat
     9001 cgcccccagc tccagcgcgc gcagcgtgac ttctgaccct ttgccggtca gggaagaaac
     9061 catcactacg ggcattggac gcaaacgcat taatttttcg aggaaatcca gtccgtccat
     9121 ccgcggcatt tcaacatcca gcgttagcac atcgggattg aatttcttaa tcaaatcacg
     9181 cgcgaccagc ggatcagggg cggtcgccac catttccatg tcgctatggc tgttgatgat
     9241 ttctgtcatg atctggcgca tcagtgccga atcatcgaca gataacaccc tgattttgct
     9301 catcgttaat ccttacttag cgcatacacc gtctgaccac gcagcgtgaa gcggcgctca
     9361 aggtggctaa agttttcaga gtgacccgca aacagcaatc cgtcgggttt aaggagcgga
     9421 acaaagcggc gcaaaatctc ctgctgggta gtttgatcga agtagatcat gacgttacga
     9481 cagaagatcg catcaaacgg ccccggcacg gtgtactgtt tcgccagtaa attcagcggg
     9541 gcaaaatcaa catagttcgc cagctcctga cgcacgcgta ccagcccttc atgcggtccc
     9601 gtccctcgca tgaaataccg ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg
     9661 cgataaatac cgcttctggc tttttccagc acttcggtgt cgatatcact ggcaaacact
     9721 ttccagcgtc cgggcacggt gcccaatgtg tcagccagcg tcatcgcaat gctgtacggc
     9781 tcttcgccgg tcgaagccgc cgcgctccat acgcgatact cgccagaacg acgccgggcg
     9841 tgatccgcga gcagaggaaa atgatgcgcc tcacggaaaa aagccgtcag attcgtggtc
     9901 agcgaattga taaacgcctg ccactcaccg ctgtgctgat tagattccag caagttcagg
     9961 taatggccga aatccgtcag tcccagcgaa cgcaaacgac gaaccagtcg gttgtaaacc
    10021 atgtcgcgtt tatggtcggc caggacgatc ccggctcgtt gatagatcaa ttgacttatc
    10081 cgccgaaaat gcgcgtcgga aagcgccagg cgctcggtca tctgtaacaa taaagacgtt
    10141 tgcccacagg gcagagatga agtcatagcg ccttctcaat cacttcagga taccactggc
    10201 gcaatatgta actgcgccga ctcatgtcgt gctacttcat gttcttcaag ggtaaatacc
    10261 gccacacgcg acgaaagatg gtcagcctgg ttcgccagtt gttccgttgc cgccgccgct
    10321 tcttctacca gcgaggcgtt ctgctgtgtc acctgatcca tctggctgac agcctgcgca
    10381 acctgctcaa tcccccgccg ttgttcttcc gaggccgaag caatttctcc cataatgtcg
    10441 ttcacgcggg tcaccgaact gacaatatca gtcatggtcg cggcggcgtt attcaccagt
    10501 ttcgaaccct gctggacacg attgactgac tcttcgatca gccctttgat ctcttttgct
    10561 gcctgggcgc tacggctggc aagattgcgc acttcacctg ccactaccgc aaaaccacgc
    10621 ccctgctctc cggcgcgagc cgcttccact gccgcattca gggccagaat attggtctgg
    10681 aaagcaatgc cgtcgataac gctgataatg tcgcctattt tttgcgagct ggtggcgatc
    10741 tcctgcatgg tgtgagtcat tgtactgacc tgaacaccgc ccgcctgcgc cgttgtcgcg
    10801 gcattttttg ccagttccga cgcctggcgc gcgttatcgg cgttttgccc taccgtggcg
    10861 gttaattgct ccatactggc ggccgtttgt gccagcgatg ccgcctgctg ttcggtacga
    10921 cttgagagat cgttattgcc tgcgacaatc tccgcgatac caatgtgcat ctcctggctt
    10981 cccttacgca catcactcac cgtcccacgc aaagcctgct gcatggtctt caggctggca
    11041 aaaatggcgg tgatctcatt acgaccatat accgcaatcg gacgcgccag attacccgca
    11101 gcaatgctgt caaaatggct accgataatg gccagtggtt gaacaatcat cttgcgcgtc
    11161 caccacagcg cactgctgat gtagatcgcc gcaacaataa tcatgctgat aaacaccagc
    11221 gccgaaatct gatagttacg ctggctttgc gcactggcgg cttccagcac atggttgatc
    11281 tccagctgcc aggcctcaaa gttaacgtca aacgcattct gtgattcctg caccggcgca
    11341 gtgaggaaat ccgaaagttg attactttca agccaggtcg cctgatgttc gagatcgttg
    11401 tgccagcggg caaaactttt ctccgtctct ttttgcaatg ccctgacgtg ctcgttgccc
    11461 gcagtcatcg ccataaaact tttaaacagc gtggtggatt gcgtcagact ggcgcgcgcc
    11521 gtcgtcatca acgttttaat gtcatccgcc ggatagctaa gcgccgtcag agtgcccgct
    11581 ttgttcagcg cggtactggc ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt
    11641 tgctgattgc tctgctcgac ctgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaaa
    11701 gacatgccgt tactgccaat ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg
    11761 gtcgaaattc gaatacgatt aaacatcaac gctccccatc aggcggcaat gaccgcgtta
    11821 gtaaaaactc gtcaaaatgt ttcccagttt ggatcttgtt cagtaattcg cagtcgtggc
    11881 tgtgccggtg gttgctcact ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg
    11941 ctggctgcca gacgaaacgc ggaaaccgct tgcgttaaac gactggcctg ttcttccagc
    12001 gcagctgcgg cggcagctga ttcctgcacc agcgatgcgt tctgttgcgt gacgcgatcc
    12061 atttccgaaa ccgccaatgc gacttgatcg atgccacggc tctgttcatc cgatgccgat
    12121 gcaatctcgc ccataatgtc agtcacgcga gtgacagcat tgacgatatt gttcattgtt
    12181 tccccggcgc tttcgaccag caccgaaccg gtatcaacgc gcgagacgga gtcttcaatg
    12241 agggctttga tctcttttgc cgcctgggcg ctgcgactgg caagattacg cacttcgccc
    12301 gccaccacgg caaaaccacg gccctgttca cccgcacgcg cggcttcaac agcggcattc
    12361 aacgcgagga tattagtctg gaaggcaata ccatcgataa cgctgataat gtcggcaatt
    12421 ttcttcgaac tatcggcgat ctcatgcatc gttttcacta caccatccac tacttttccg
    12481 ccgtgctggg cagtatcgga ggcactttgc gccagttgcg aggcctggcg ggcgttatcg
    12541 gcgttttgct tcactgtcgc agtgagctgc tccatgctgg cggcagtttc ttccagcgcg
    12601 gatgcctgct gttcagtacg ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta
    12661 ccggcataga tggcatctga accttcacgc acatgagtga cggtgtcagt caaagagcgt
    12721 tgcatatgtg aaacgctctg cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg
    12781 gtattcgcca ggttaccact ggcgatttcg cgaatgtgag caataatttt tgccagcggt
    12841 gtaagcaaca tacggcgaat gccgtaccac gccaccagca gaatcaatac caccaccagc
    12901 gcaataaccg ccagttgcca ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata
    12961 tcgcgataca gtttttcact gctgagggcg tactgagcaa acgcttcgcc cattgcattt
    13021 tgcattccct gggttggctg agcgaaataa gctccagtat tgccataatc aagataatca
    13081 atcagttcag ttaacgctgt gtgatagttt ttatattttt catcaatatt acgactggta
    13141 gcgaccattt caggtaacgg tgccatgctt ttgaattttt tataatgcgt cgctgcctgg
    13201 gccaatgttt tcctggcgct atcgagcaat tcaactttgg cgttactttg ttgattggag
    13261 gaatccatca tcatccgcac cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa
    13321 tcccaggttg acgtcagctc gccctgctgt tcccgtaatt gattggaaac cacaaagctc
    13381 ttctggctat ggtgaaggga agaaaaaaac agactgccgg aaataagctg taacagtgcg
    13441 aataccccca gcaccattac caacagcgtg actacgcgga tacggttaat cataaggcac
    13501 cttcctgaaa acaagttgat ctcgttatcg gcaaggaagg gggaaacttt attgctgatg
    13561 ccacccgccg cgaaactgaa ataaaaaacc cgatgcgcag gtcatcgggt tcattgcaat
    13621 tgaggaaatc gggagaatta cgccacttct gacgccgcgc tatctaacag cgccatctct
    13681 tcgctgttca gcagtttttc gatgttcacc agaatcaaca tccggtcgcc cagtgcgccc
    13741 agtccagtga gatattctgt tgaaagcgtc acggcaaatt ccggtgccgg acgaatttgc
    13801 tccgccgtca atgaaagcac gtctgaaacg ccgtcaacca cgatgccgac cacccgctgt
    13861 ccgagattca ggacgataac taccgtgttg tcgttatagt ccacatccac ctggctgaac
    13921 ttaattcgta agtcgacaat cggcacaata acgccgcgca gattcgtgac gcctttgata
    13981 aacgctggcg tgttcgcaat ccgtgttacc tgatcgtagc cacggatctc ctgcactttc
    14041 agaatatcaa taccgtactc ttcatcacca agggtaaata ccagaaattc ctggcctgac
    14101 ggctcgctgg ccagctttgt tacattcgtc ataccggtca tattgttacc tttttactca
    14161 ttcaggcggc ggtgttcgcc atacgttgtt cgcggtttat cgcctgcaag gcggagacat
    14221 caacaatcag tgccacgctg ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt
    14281 tgcgatagtt actttcaagg tttttaacca caacctggtg ttgaccaatt aattgatcca
    14341 ccagcaaggc gtagcggcga ccgccacttt gtaagatcac cacaattccc tgggtagctt
    14401 cggttttcgc gcccgcgacg ttgaacactt tccacagttc gacgatgggc agatattcac
    14461 cccgcacttc cagcacccgc tcgccgcctg ccagtggatg cagatcggct tcacggggtt
    14521 gcagtgattc cataacagca ttcagcggca gtatgaaaac ttcatccgca acgcgtacgg
    14581 acatgccgtc gaggatggcc agcgtcagcg gcagtaaaat gcggatcgta gtgccagtac
    14641 cctgcttcga ctggatttcg acatgaccgc ccatctcctg gatattacgt ttaacgacgt
    14701 ccatgccgac gccgcgcccg gagacgtcgg tgacctgctc cgccgtggag aagccagggg
    14761 caaatatcag catcgcgact tcgtcgtcgc tcatgttttc gctgacggtc agaccttgcg
    14821 aggccgcttt tgccagaatt cgctcacggt ttagccctgc accatcgtcg gtcacttcaa
    14881 tgcaaatgtt gccgccctga tgttcggcag aaagaattaa atttcctacg ctgtttttac
    14941 ctgcggcgag ccgtttttct ggcagttcaa taccgtgatc gaggctattg cgtaccaggt
    15001 gggtcagcgg gtcgataatg cgttctatca ggctcttgtc gagttcagta gaactgccca
    15061 ccagcgtcag ttctacctgc ttgccgagtt ttcccgccag atcacgcacc agccggggat
    15121 aacggctaaa gacatattcc atcggcatca tgcgaatcga catcactgat tcctgcaaat
    15181 cacgagcgtt acgttgtaac tgccccatgc tggtaatcaa atcaccatga ttaaccgggt
    15241 ccagttcgct ggaacgctgg gcaagcatgg actgggtgat gaccagctcg ccgacgaggt
    15301 taattaattg atcaaccttt tctaccgcta cacggatgct ggtggattca ctgctgcgcg
    15361 tcgttttttc ccgctccacg cgaccggttg gcgcttgttc ggctgccagt ttaagcactg
    15421 gtggggtgga tattttgggc gagacttcta ttgtttcaaa ggtaatctga tcggcttcaa
    15481 tcacaaaaca gagtaccgct gtgatgtcat cttcggcgat gtcgcccggt aatattgccg
    15541 agagagaatc cgtccctttc accacgtcag ttaacgttgt cagatgcccc agctcttctt
    15601 ccagcaggtc gacttccccg gccttcaggc gcgaaaggat aattcgtcgc ggcgactgac
    15661 tgcgactctg ctcatcttgc ggttcacttt tggcaaccac acttaatcgg gtcactgcgg
    15721 atggcgtttc gtctttcgct tctaatgcca gttgacgcaa ggcctggcag atataatcga
    15781 agctggcggc atccggctct tgcgactgtt tataagcgtc gagctgttct tgcatgatgt
    15841 ccttcgtttc caaaaacaga ttgataatgt cggtgttgag ttgcatctca cctcgtctgg
    15901 cttcgtcgag caggttttcc atcagatgcg tggtttcctg caaaacgctg aagccaaagg
    15961 ttcctgcccc tcctttgatc gagtgggcag cccgaaagat ggcattcaat tgttcggcat
    16021 ctggcgcttc cggctgcaaa accagcagat gctgctccat gtcagccaac agttcgtccg
    16081 cttcatcaaa aaatgtctga taaaaatcgc ttatatccat gctcacgctg tcacctcggt
    16141 tcggctgatg gcattgtggg aacactgacc tgtggtgcaa cctcaggttt ttccagggcg
    16201 cttactggct cattctggct ttcggcgttt tcatgcaaaa tggcctcttc ggcttgtttg
    16261 ttcagtacca gcaggctgat gcgacggttg acagcatcat caggtccgcg atcgcttaag
    16321 cgcatcgttg ccgccatgcc gacgacacgt aacactttgc cgctatccaa cccgccaacc
    16381 atcagttcgc ggcgggatgc attggcccga tcggcagaaa gctcccagtt gctgtaacct
    16441 ttctcaccac tggcgtaggg gaaatcatcg gtatgacccg agaggctaat tcggttggga
    16501 ataccgttca gtacgggcgc gatggcgcgc agaatgtcgc gcatataggg ttccacatcg
    16561 gcactgccag ttctgaacat cgggcgattc tggctatcga tgatctgaat acgtagacct
    16621 tcctggacca gatcgatttt gagatgtgga cgtaacgccc gcagtttcgg atcggactct
    16681 atcaattggt cgagatcgcc acgcaatttc cgcaatcgac ttttctccat gcgttttttc
    16741 agctcttcga tgttcggctg cttattcact tccccctggc tttgggtgta atcatcaccc
    16801 ccaccgggaa ttgggctttc actattagaa atgcgatcgc cgcccgtaac cgcagtcgcc
    16861 agtggagtcc ggaagtactc cgcaatctga atcagctctt ttgggctgga gatggagatt
    16921 agccacatca ccagaaaaaa ggccatcatc gcggtcataa agtcggcata agcaatcttc
    16981 cacgatccat gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac aataatcgga
    17041 tgcgcttgat tcttcatgct tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac
    17101 gcacatgctc ttccagttca ataaacgacg gacgttcgct ggaatagagc gttttgcgac
    17161 caaactcaac ggcgataggc ggtgcgtaac cgttcagatt agaaagcagt gtgactttga
    17221 cgcactgcat cattttgctg gtttcggcgc ttttctgacg taaaacactc gctaatgggg
    17281 aaataaatcc gtaagccaat aaaataccga ggaaagtccc caccatcgca tgtgcgataa
    17341 gcgcccccag ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg acccccatca
    17401 cagccgcgac aataccaaac gccggaagtg agtccccgac cagcgccaga ctgttcgccg
    17461 ggacttctgc ttcgctttcg tgcgtctcaa tctcttcatc catcagggct tcgatttcga
    17521 aggtgttcat gtgaccgctg ataatcagac gcagataatc gacgataaaa tcaagcatga
    17581 cgctatccgc aaggatgcgt gggtagctgg cgaagatctc gctctcacgg ggattttcaa
    17641 tatcacgttc cagcgaaaac atccccatct gccgcgattt cgccatcaac cgataaagca
    17701 gagccagcag atccatatac attgctttgg tgtatttgga gcgacgaaac agcaacggca
    17761 gcgccttcag cgtgccttta atcgctttgc cattattgcc gacgataaac gacccaatcc
    17821 ctgcaccggc aataatcacc agttcagcgg gttgatagag tgctccaagg cttccaccgg
    17881 tcatcaaata accgccgaaa actgtaccga gaacaaccag gtaacctaat aagataagca
    17941 cgacatcatc cttccactgt tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc
    18001 gattggggca aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt gcgccacacc
    18061 gtataagtta aacagcctgt actctctgtt catccagcag ttgtgggata atatcggcag
    18121 gattctggga aagtttacgt ctttttactg cccgggatgg cggctgacat aagctgcagg
    18181 caaagctgcc aacaggctgg tgagcgtggg taataaaatt gccgccgcag cagttgcagc
    18241 tggaaagttg cagtaatcca ctttcaacaa accgtaccaa tgtccaggca cgggttaatg
    18301 ccagcagcgg tccgtcttct gcttgcgggc actgttcaag gtataaacgg taggctttga
    18361 tcactgcatc gacgccatta cacaaaccgg ttttcagtaa aaactgccat gcattacaga
    18421 acatcgaagc atgaacgttt tgttcccagg tcataaacca gtcggttgag aatggcagca
    18481 tgcctttcgg cggtgggctt ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac
    18541 ttaactgtgt ttcgctttcc agcatctgca aacgagcgcc cagggtgatc aattccattg
    18601 ccagctgaat atcccgcgct tcctgaacaa tgcttttttc actcatgatc aggccctttt
    18661 cttgcgcagc gcttcttcag gctgattaac atcattcagc aagcgtgttg agagcatgat
    18721 gccggtatga atttgctgga gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt
    18781 ctggtggctg tcaaaacgaa agtgacaaac cagttgattg gtttctgcca gcttaaccat
    18841 ttgcggaaga gtcagtgccg ctaacgttgt cgccatttct tcatttatgc cgagacgaaa
    18901 cattgcggac gctttgtcct gaacaatcag acgctgtgca agtagtaaat atgacaagtt
    18961 gatgtcataa atgtgtttca gcaactcgga ggtatgcatt attcccaccc agaataacca
    19021 actttatttt tatgcggtct caccgcaccc cgtgatgtcg ccgggaagcc ccggtaaaaa
    19081 ataattagca ttagaatagt tgcgataagc tgcaacaggc agagcccctt tttgggattt
    19141 aataagtcct tacaaataga aggggttctt tacacttatc taagattttt cctaaatcga
    19201 cgcaacttta ctcgtcacta cactcacata caacggaggg gggctgcgat tttcaataat
    19261 gtgtgataca gatcacacaa aacgctcaat tacttaacat aaatgtataa gtcatacttt
    19321 tgttttgggt gtatttcaat ctgttaaaaa gtttttcgcg acgctagcaa gcaaaaatga
    19381 aacaggaata atcgaaatgg gatgttgcgc acagttaaaa taactcaccg taaataatca
    19441 tctgctataa ataattgctt taatgcaata ccagataagc tatttttaaa cagacactta
    19501 ccgcacaaca aactaattaa atacaaagaa aggcaacaat caacttaatg ttaataaaat
    19561 gttttacata tattaaccac ttgtttaata tggagaatca gtacattatt ttcttataat
    19621 tacagttgaa atcatatgat caccaagagt gattgattaa ttgtctttac taataatcag
    19681 actaatattt acctgtttga ctgagttggg attgcgtcga ttctccatta ggagtaaagc
    19741 tttaatgtca cctgaagttc acagaataag gaacaggtta tgagctatag caatattctt
    19801 gtcgctgttg cggttacacc ggaaagtcag caactgctgg caaaagcggt atctatcgcc
    19861 aggccagtaa agggacacat cagtttaatt actctcgctt ccgacccgga aatgtacaat
    19921 caattagctg cgccgatgct tgaagattta cgcagcgtga tgcaggaaga aacgcaaagc
    19981 tttcttgata agttaattca ggatgcgggg tatcccgttg acaagacgtt tattgcctac
    20041 ggtgagttaa gcgaacatat tctggaagtt tgtcgcaaat atcatttcga tttggtgatt
    20101 tgcggtaatc acaatcacag tttcttttcg cgagcatcct gctcggcgaa aagagtgatt
    20161 gcctcaagtg aggtcgatgt gctgttagtt ccacttacgg gagattaacc gctcctacgc
    20221 aatctttgga aaggtagcaa ctttatcgcg ctgctggctt tccgcgcttc gcggaactat
    20281 ctgctttagg tcgctaatga agcactcctg ccagtggtta atatcgtttt tcacgataac
    20341 gtccagcatt tctgcatgac gggaaatacg ttccgccagc gacatagtca atgcacgatc
    20401 cagcgcagct gcaacttcgt cacgatcgta gggattaaca attaacgccg acgttaactc
    20461 gtttgctgct cccgcaaatt gcgaaagaac aagtacgccc gggttggctg ggtcctgagc
    20521 ggcaacatac tcttttgcca ccaggttcat cccgtcacgc agtggcgtca ctaagcccac
    20581 gtcagagtag cggaatattt tcatcagtaa tttacggtca aaatgctgat tcaaataata
    20641 aagcggcgtc cagcctaatt gcccatattt accattaatt cgtccggctt cattttcgag
    20701 ctgatgacga atatcctgat aggcttgcac atcaccacgc gacgttggcg cgatctgggt
    20761 ataacgaatt ttaccatgat gctgcggata tttttccagc aacgcttcat aggcgagaaa
    20821 acgctccggc aaacctttgg aataatccag ccgttcgaca gaaaagatat tttgtacgtt
    20881 tttcagttcc gctttaagtt gcgccagttt tggcggcagt ggcccggcag cctgtttggc
    20941 tatttctttt ggctcaatgc caatcgggta aacttctgtt cgaaacgctt tgccccaggc
    21001 tgtatggctt tttgcgctac gcgtcgtgac gcgggtcagg ttagaaaggc aatccaggaa
    21061 cgccagacga tcgttttctg tctggaaacc cagcaaatca taatcacaaa gctgttcaag
    21121 caaggtgtca tatgtcggca gcgcgttgaa gatttccggt gtcgggaaag gaatatgcag
    21181 aaagaaacca atgcgattat tcactccccg tttgcgtaat tcatgcgcaa atggcaacag
    21241 gtgataatcg tggatccaga taatgtcatc atcttgcaac agcggcaata atttatctgc
    21301 cagcaacgca tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcaccagatc
    21361 gagccgataa tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc
    21421 aaggtcctgt tcgctgaggt taaaagaggc ccacgtaatg ttaccctttt tcaccttttt
    21481 tagcggctga tcctcattcc ctgtttcgcc actccagcca aaccacagtc cacctgcggc
    21541 tttcagtgcc cccagaatgc caacggcaag gccaccggca ctggcggcgt gctcatctgg
    21601 tggtgcaatc cggttagata ctacgactaa acgactcata gtcatcactc ctgttatttt
    21661 ctcttttttg ttgtaatgcg gtggttatca tttcaagcca gctccagaca tccggtacgc
    21721 ccgccagtcg ccatgatgcc tgagttgcgc ctgtgccaat ttttactgac attccgccca
    21781 gccggttaac gacagcgaag ccagattcat cggttaaatc atcgcccaga aataccggcg
    21841 ttcgcccgat aaagggagct tcctgcataa aggcagcaat cgcctcacct ttactggtgc
    21901 ctcgcggttt gatctcgaca acacactttc cctgctgtaa cgccatttgt ggccagatct
    21961 gagtaatacg ttgcgctaat gtcattaatg cgtcttcatg ctgcggagcc tgacgataat
    22021 gcagcgcaaa ggccatccct ttcgtctcca gctccgcacc gggatactgg gcgatagccg
    22081 tatgcagttg cacgctaata tcacgcgcaa tcgcatccgg cagatgaacg atatgcgttt
    22141 taccattgat gtcacggcgc tccgccccat gcacgcccgc taatgggaag cgataaggtt
    22201 ttgccagtgc gtcaagctcc accattgagc gccctgatat caatgccaat gcaccatcac
    22261 ttgcgattgc cagtagctgt agtccttgca gaatattgtc aggcacgacg acctgatcgg
    22321 gatgcggttt gatttccgcc agcgttccat cgagatcaaa aaaccaggca tatttcgcgg
    22381 atagttcagg ggtttcggtt aacggttctg tcacccggtt ctcctccttc tcttttctta
    22441 ttgtgttgcg cagaacgcgc tttacagaca tgtaagtata gacagtgtga cgggggtcgc
    22501 catttgtaac gaagaacagc caaccgttgt ggcggctggc tgtggtggga aaaaacgtta
    22561 aattgttgtg gaaaaaagca tcagacagtg cgtttcgctt tttgcttgta acggtcgaag
    22621 atcaccgctg ccagcaggat taagccgcga actacgtact gtgcgaaagg agaaatatta
    22681 agcaggttca tggcgttttc cacggtgcct aaaattaaga tacccgccac cacatatgag
    22741 atttttccga tgccgccttt cagagaaacg ccacctaaaa cgcaggcgga gatcacaatc
    22801 agctcatagc caatcgacgt cattggctgc ccactggtca tacgtgaagc cagaataatt
    22861 ccggctatcg ctgataccag gccagagaga acaaagataa taattttggt gcgaacaacc
    22921 ggtacacccg ccagacgcgc agcctcttcg ttcccgccaa tcgccagggt attacgacca
    22981 aaggtggttt tattcagcag caagccaaag atgatcaaac acgcgacggt gagccagatt
    23041 ggcgcaggca gaccaaacca gttggcgtaa ccaagggcaa agaagctttc atcttcgata
    23101 ccgaccgctt taccgtctga aatgatgtac gccagacctc gaacaatctg catcgttgcc
    23161 agtgtcgtga tcagggcatt tattttgagt ttggcgataa caaagccatt gaccaggcca
    23221 cagagaacgc ccagcaacaa acccgctgcc acgccaatcc acaggctttc agtcaggttg
    23281 ataaccaccg ccgtggtgac acccgcacag gcaattacgg aggcgacaga aaggtcaaag
    23341 tcaccggaag caaggcagaa caacatgcca caagccacca tccccgacat ggaaattgcc
    23401 aggcccaacc ctttcatatt aatgaaggtg gcaaagttcg ggacaaaaat ggcacaggcg
    23461 ataaaaagca ccgcaaacac caccagcatg ccgtactgat cccagatacg cccgaagctg
    23521 aatgacgact taggtgcgcc agaacccgat gtagaaacag aagacatcat actctcctta
    23581 ctcaggcaac tgcctggctg actttaggca tcgcaaggct cagtgcctga cgctcatctg
    23641 cctgctcgtg taacaattca ccggcgattt caccttcgcg catcaccaca atccggtcgg
    23701 caacgccgag gacttcaggt aagtcgctgg aggcaaacag caccgccaca ccctgcgccg
    23761 ccagcgcata aattacgtta tatatttcgt gcttagcgcc aacatcaatg ccgcgcgtag
    23821 gttcatccag caaaatgacc ttcatctctt ccgataacca gcggcccaga atggcttttt
    23881 gctgatttcc gcctgagaga ttcatgatca gttgctcagc gcccggcgtt ttgatgttaa
    23941 gcgaacgaat gtggtgatcg gcattgtttt cttcccaacc gttgttgatt acacaaccgc
    24001 cgagcacatg tttacgtctg gcactgatgt tgatattgtc gcgaacggag tgcacaggaa
    24061 taatgccttc ggctttgcga tcttccgggc agagcatcat gcctgcggca atggcgtggc
    24121 tcggtttacg aatatcgatc ggctgttggt cgatataaac ctgaccggcg gtaatttgcg
    24181 taccgccaaa catgcctttc attaattcgc tacgccctgc cccgaccaga ccaaacagac
    24241 cgacaatttc tccactgcga accgccagac ttattggcgt acgcacgcct ggtgctttca
    24301 cagcatcaag acgcaggcgc tcctcgccat aactacgcgg ttgccagccg tagatatcac
    24361 cgatgtcgcg cccgaccatc gcctgcacca gggcgtcgtg gtcaacctgc tgcatatcag
    24421 taaaggtttt gacataacgc ccatctttaa agacggtgat ggcatcgctg agggcaaata
    24481 tttcttccat acggtgagaa acgtataaga tcacccgccc ctcttttcgc agttcacgaa
    24541 taacgcggaa aagattgtcg atttcacggg cagagaggga gctggttggc tcatcaaagg
    24601 cgataatttt ggcgttacgc gccagcgctt tggcgatttc aaccatctgc cactgaccaa
    24661 tggagagata tttcagcggc gtgtccgggt caatatccat accaagatgt ttaagttgta
    24721 aacccgcctc ataattcagc aatgagcgat tcacaatgcc gcctttatgc ggcagctggc
    24781 cgagatagat gttttccgcg acggtcattt ccggcacgag atgcagttcc tggtaaataa
    24841 tcgccacacc cgcgttaagt gctgcggtcg tgtcggaaaa ggacatttcc tgcccattaa
    24901 tcactacaga acccgtggtt ggcgcatagt tgccgctgag gatttttaag agagttgatt
    24961 ttcctgcgcc attttcaccc atcaacgcat gaacctgacc ggcatagcaa tcaaaactaa
    25021 tatccgtcag cgccttaacg ccgggaaacg ttttaccgat gccgcgaaat gagagatacg
    25081 gggtagactg ttgcataacg tctccgtgaa tcactggtcg ttcacacccc ctctgcatca
    25141 tgcagagggg aatttttctg gcaaattact taccgcctaa accttttttc tccagttctt
    25201 ctttaaagtt gtcacgcgtg atcaatacca cgtcggtaac ttcggtaaat tttggcggtt
    25261 caacgtcttt tgctacccag ttgtaaagca tttcgctgga tttatagcca tgtacgtccg
    25321 ggcttggcag cagggaaccg tagaagccgg ttgcctgtgc cttagacagt tcgctcaccg
    25381 catccacacc gttaataccg ataccgatga tatcggccgc tttaaagccc tgaccttccg
    25441 tcgcgcgtac gccgccaagt acggtgctgt cgttcatacc gacgatcagc caatgtttaa
    25501 cttccggatg ttgaaccagc attgagttgg cagcgtcaaa tgcccccggg atgtcgttag
    25561 atttggtagg tacctgataa atttgttttt ccgggaatcc tgcagctttc agcgcatcca
    25621 tagaccccgt agtacggcga cgagccgtat ccagttcgtt ggcggtaatc gccatcaccg
    25681 cgctttcttt gacatcccag ccacgtttct gcatctcttt atacagttcc tggccctgac
    25741 gttcgccaat tttggtcgct gccatcatca ccagcggaac ggtatccatt ggcttgcctt
    25801 tggcgttaac aaactggtca tccacggcaa tgactttcat atcgtagcca cgcgctttcg
    25861 ccacgatggc agagccgagt ttcgggtccg gagtacaaat aacgaaacct tttgcgccac
    25921 tggcagccag gctatcgata gcgttcaatg ttttttcgcc atccggcacg gcaatcttaa
    25981 taacttcaaa ccctaaatcc ttcccagctt tatcggcaaa cttccattcg gtctggaacc
    26041 acggctcttc cggttgcttc accaggaaac cgagcttcag gttctccgcc atagcggatt
    26101 gtgacataac ggctgccaga ccaatggctg ccagggcttt agtaaatttg tgcatggttc
    26161 tctccagctt tagtgtcgtt ttgtgtaggg caaaaacgaa tgacattcgt taaattaatc
    26221 ggaaaacaaa gcattacctt ttaactaaaa gataagtgac tgtgttgaca tagttttagc
    26281 gagaaattaa ttctccatag gagagcaata tcacatcgca gaattacagt gagaacgtgc
    26341 ataaatttag cgggaaaaga cataaggaaa agccaatttg tctgagaaat tgtctagtgc
    26401 acagcagatt aatccataag attagcctgg aaatccttgt tgtctttggt acccagcggg
    26461 atgtcttttt tttaaccagt caataggcca cattacctgg cgttgagttt ttgaaagggt
    26521 gtaataaccg caactcaaag atgtggaaaa agcgcgtcat tcatttcgcc attaattatc
    26581 accgtgctca ttaattaaca gaacacgtat actgagagcc ataccgcaaa aatgaaaaaa
    26641 cgttttaaaa aatcattgct tcatcatgaa ttcaaattca ccgattaata tcaacaagat
    26701 acaaaaagca ctatcattaa aattcattgc agttacattg atttcatcaa tgaaatgtaa
    26761 aaatatataa acttgatgat ttaagcattt tcttataccc attcagacgt tattcttatt
    26821 tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt
    26881 ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc tggatataag
    26941 gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga
    27001 gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa
    27061 cggcaccgcc actttccttc gcgcccaggc acagagtaac gtgacccaaa tgatgcgcat
    27121 gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttcccgg
    27181 tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg
    27241 ttcaagcacg ctggcgcagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt
    27301 caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggcctgttgc tgcaaaccat
    27361 tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca
    27421 tgttctgaat gtcgtgtcgc atcagctgca ttgatcatac atcggcgcta atgcattgcg
    27481 ccgatgaagt ttttgagaaa ccgctgcctc atctttttga agcagcggtt tttttaatgg
    27541 gattcgccct gtggggtaaa cttgagttca atcagcgcga tggctttttg gattgcccgc
    27601 atggtgaccg ggtctgcggc ggcgggatgg ttagtaaagt cgatattctt cagctggctg
    27661 gacatttttt cacgaacttc aacgggcgcg attacatcga gaacatccag aatttgtttg
    27721 ataaccaact ggcaagcaac cacatcagaa accagttcct gatcggcatt cagcggctgg
    27781 gacatcgtaa actcctgata gcattttgaa agccgttata gtagcggctt cacatcttca
    27841 gcgatagtca catccaccgt catcaggaca caaaaaaacc tgccggagca ggttttttgt
    27901 tatcggaaca tattgcctgg cggtacgtct ttgaacgtct tgcaatagtt gttgaacata
    27961 cttttcagga ttttgcgcag tttcatcgcg gcactccgac catttgttat acaggtgtta
    28021 ttgtctttgc gcttataata tgacaaccat cacaaaaatc aatctttatg tgatgcaaat
    28081 cacataaata cccctttaat gttataaaaa tgataatcaa aaagcagccc cccctttctg
    28141 acacatacag atggcgagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc
    28201 gctgtaacgc gtaaaatact gctttctttt actcttctgg ctggaccatg agacttctga
    28261 ttctgactct ttcattaata acgctggtgg ggtgcacagt tactcgccag gctcatgtgg
    28321 gcgaagttga cgcggcaaca ggtattgttc ggttggttta tgatcaggct tttttgcagc
    28381 atgcccgtac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag
    28441 gctataccca cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt
    28501 ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg
    28561 tctttctcta aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa
    28621 tgcgttttat tcccattcgt atttttaata aatgaagttt atattttacc ttttgcaaat
    28681 aataaaataa caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat
    28741 catgctgaaa ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc
    28801 ttcactgctt tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc
    28861 cgcgttcctg cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta
    28921 tctgactgat accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata
    28981 ttcctcactt gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa
    29041 aattaacgaa ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct
    29101 gcaatggtat gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa
    29161 attaagtctg gcaggcaaaa gcggcgaagg tttgtatttt atcgacaaag aactctctac
    29221 cctcgacaca caaaactaat gttctccggc ggcaggttta ctgccgccgt ttcaaatcag
    29281 tgacggcaaa tcttactctg atcggtcgaa aaaccttctt ccgaaggtat aaatttgcct
    29341 tttgctgtca gaaacgctac cagctctccc gctgtcatcc cttctgccga gcaagtgtga
    29401 aaacgtgcct gttcaccaaa acgcgcttta atcgcagctt ccagactggc atgcgtatat
    29461 tgctcacctg attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtt
    29521 cctcctcaaa aaaaaggcta gcgtagcgat tgccgcttac caagactttg cgccagcgca
    29581 ggactgaacg cattttattg tacatttata tttacaccat atgtaacgcc gatttgacga
    29641 agcagccgtt atgccttaac ctgcgccgca gatatcactc ataaagatcg tcaggacaga
    29701 agaaagcgtg aaaaacagaa ctctgggaag tgtttttatc gtggcgggaa ccacaattgg
    29761 cgcaggcatg ctggcaatgc cgctggctgc tgcaggtgtt ggttttagcg tcacgttaat
    29821 cttgttgatt gggctttggg cgttgatgtg ctacacggcg ctattactgc tggaggtgta
    29881 ccagcatgtc ccggcagata ccggcctggg cacgctggca aaacgctatc tgggacgcta
    29941 cggtcagtgg ctgacgggct tcagtatgat gtccttaatg tatgctctga ctgcggcata
    30001 catcagcggt gccggtgaat tgttggcctc cagcatcagc gactggacag gtatttcaat
    30061 gtcggcaacc gctggcgtgc tgttgttcac ttttgttgcc ggtggcgtgg tttgtgtcgg
    30121 gacatcgctg gtcgatttgt ttaaccgttt tctgttcagc gccaaaatta tttttctggt
    30181 ggtgatgctg gtattgctgc taccgcatat tcacaaagtg aatcttttaa ccctgccgtt
    30241 gcaacagggg ctggctctgt ctgcaatccc ggtgattttt acctcgtttg gttttcacgg
    30301 tagcgtgccg agtattgtca gctatatgga tggcaacgtt cgtaagctac gctgggtgtt
    30361 tataaccggt agtgcgatcc ccctggtggc atatattttc tggcaggtgg caacgcttgg
    30421 cagcattgat tcaacaacct ttatgggatt gctggctaat catgccggat taaacgggct
    30481 gttacaggcg ttacgcgaaa tggtggcctc tccgcatgtt gagctggcgg tgcatttatt
    30541 tgctgattta gccctcgcca cgtcattcct cggcgtagcg ttaggtttat ttgattatct
    30601 ggcagatttg tttcagcgtt caaataccgt tggtggacga ttgcaaactg gtgcaattac
    30661 ctttctgccg ccgttggcgt ttgcactgtt ttatccacga ggatttgtga tggcgctggg
    30721 ttacgccggt gtggcgctgg cggtactggc attgattatc ccttcgctgt tgacctggca
    30781 aagcagaaag cacaatcctc aagcgggtta ccgggtcaaa ggtggtcgtc cggcgctggt
    30841 ggtggtgttt ctctgtggta ttgctgtgat tggcgtgcaa tttttgattg cggcagggtt
    30901 gttaccagaa gtggggtgat tagatcttat tgggtgccag aattaacgct gacacccaat
    30961 ttgatctctt aatgcaggca gcactgctta aatttcttac cactaccgca cgggcaagga
    31021 tcattacgcc ccggtttctc ttctgctttg atcggttgct gaacagcttt ttcctgcgga
    31081 tgcgccatcc agtacgcatg tagatcaagc gccgccagtc gaatggcatc tacgctctct
    31141 tcaaacgctt ctggcgacat cttctctaac cgctcgaagt tttcctcagt accgtgcagc
    31201 gcaatcgcct ccagcgctgg ttttaacgaa tcgggcaacg ttgaccagtc agaaagtgcc
    31261 acgccccgca tatagccaaa gcaccactcc tcaacaatcg tcagctcgct gccatcgact
    31321 tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt cgttcagacg ctctgcggta
    31381 tcagccatat gctgaaaagc caggttcata aagcgcgtca tctctttctc tgacgcccag
    31441 cgtggcacat agtcagcccc accccacacg gcaaccagcc actgtgccgg ttcaatctct
    31501 tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc aaggatggcg
    31561 tggtcagtgt tgtatttggt cagaatatcg tccagccatt ccaactcact ttcgtttaac
    31621 ggtcccgttt tcatacgctt ttccttgtgg ttctcaactc gccagcacct atcttacatg
    31681 ccggtccgta tcagagatac tttttgagtg gctttgctgg tgattaataa ttaaggaggg
    31741 tgtaacgaca agttgcaggt acaaaaaaac cacccgaagg tggtttcacg acactgctta
    31801 ttgctttgat tttattctta tctttcccat ggtacccgga gcgggacttg aacccgcaca
    31861 gcgcgaacgc cgagggattt taaatccctt gtgtctaccg attccaccat ccgggctcgg
    31921 gaagaaagtg gaggcgcgtt ccggagtcga accggactag acggatttgc aatccgctac
    31981 ataaccgctt tgttaacgcg ccaaattctt caggcctttc agccagacat ccgcttgacg
    32041 ccgatgtctt ttaaactgga gcgggaaacg agactcgaac tcgcgacccc gaccttggca
    32101 aggtcgtgct ctaccaactg agctattccc gcattcatca agcaatcagt taatcacttg
    32161 attttattat cgtctggcaa tcagtgccgc cgttcgatgc gttgcattct acttacctgg
    32221 cgcgatgagt caacgatatt tttcaccact tttgatcgtt tgctgaaaat tgcgccgaaa
    32281 cgatcactga tcaagcaaat ctgcacgcgc agcgctcaaa tattgcaaca ttgaccacag
    32341 agtcagtacc gcagccacaa agaaaagtgc aataccggcg tactcaaccc aaatgttcgg
    32401 acgccacagc agccaggcca gcgccaccat ctgggcagtg gttttcactt tcccaatcca
    32461 ggagacagcc acgctactgc gtttacccaa ctccgccatc cattcgcgta gcgcagaaat
    32521 aataatttca cgggcgatca tcgttgctgc cggtaaggtc acccaccagc tgtgataatg
    32581 ctcggttacc agcaccatgg cgatagccac gagaacttta tctgctacag ggtcaaggaa
    32641 agcaccaaac cgggtactct ggttccagcg gcgtgccaga aaaccatcga accagtcagt
    32701 caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc gaccaggtga caggcagata
    32761 aaagaccaat acaaagaatg ggataaggat gacacggaac agtgtaagca acgtagggat
    32821 attaaattgc ataatgacgg gtaactatct gttgtcagta agattacccc tatgttgcta
    32881 cagagacatc aatgtttcaa cgaccagaag atcttttctg ccagaccttg cgaaataccc
    32941 ggcacttttg caatttcctc aacgctggcg ttacgtaaac cttgcaaacc gcccatatat
    33001 ttcaacaaca tttgccgacg ttttggcccg acgccttcaa tggtttccag ggaactggta
    33061 tttttgacct tcgcccgttt cttacggtga ccaccaatcg cgtgatcgtg agattcatcg
    33121 cgaatatgct ggataacatg cagcgcggga gaatctggcg gcaaactgaa tccctcacct
    33181 tccggctcaa agaacagcgt ttccaaccct gccttcctat ctgcaccttt ggcaacgcca
    33241 agaagcagcg gatgattttt atcccatgag acatccagtt cggcgaagac atttttcgcc
    33301 tgcgcaagct ggcctttgcc gccgtcaata aggatcacat ccgggatctt actgtcgtcg
    33361 atggctttac cataacgccg acgcagcacc tgattcatcg ccgcataatc atcgcccggc
    33421 gtgatgccag taatgttata gcgccgatac tccgcacgca gcgggccgtt agcatcaaac
    33481 accacacagg aagcgacggt ttgttcaccc atggtatggc tgatgtcaaa gcactccatc
    33541 cgcttcactt ccggcaattt caacacgctg gcaagcgcgg tcagtcgctg gtgaacggta
    33601 gattgctgcg aaagtttgct ggttaaggcc gtcgccgcat tggtgcgcgc gagtttcaga
    33661 taacgcgccc gatcgccgcg aggtttggtt tgaacattaa tcttgcgtcc cgccagttct
    33721 gaaagggaat cggcgagcag cgttttatcg ctaagattaa aatcgagcag gatctcaccc
    33781 ggtaaggtgc gcatctggct gccttgtaaa tagaactgac ctacgaaggt ttctaccacc
    33841 tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc tgcggctgcc gagcactttg
    33901 ccctgacgaa tgaacaatac atggacacaa gccattcccg catcgaacgc cacaccaata
    33961 acgtcgaggt cgtcgccggt attggacaca aactgttttt cggtgacgcg tcgcaccgcc
    34021 tgaatttggt cgcgaatacg tgccgcttct tcaaactcca gattctggct ggcagtttcc
    34081 atacggctaa tgagttgcgt aagcacctga tcatctttgc cagacaaaaa caggcgcaca
    34141 tactcgacct gctgagcgta ttcttcttca ctcaccagtc cttcaacgca cggtcccaga
    34201 cagcgcccta tctgatattg cagacacgga cgcgagcgat tgcgataaac actattttcg
    34261 cactggcgaa tggggaatat cttttgcagt agcgccagtg tttcacgtac ggcatagcca
    34321 ttcgggaacg ggccgaaata ttcaccttta gcatgcttcg caccacgatg catcgccaga
    34381 cgcggatggg tatcgccact caggaagata aagggataag atttatcatc gcgtagcaaa
    34441 acgttgtaac gcggctgata gagtttgatg tagttgtgtt ccagcaacag cgcttcggtt
    34501 tctgtatgag taaccgttac atcaatttgc tggatctggg cgaccagcgc ttcggttttg
    34561 cgcgaagcga ggttgctacg gaaatagctg gaaagccgtt ttttcaggtc tttcgctttg
    34621 ccgacataga taaccgtacc accagcatcg tacatgcgat aaacgcctgg ctggctggtt
    34681 acggttttta aaaacgcttt tgcgtcaaac tgatcactca ctgacttgat aatgtctccg
    34741 cattacacag accatggcga attgccaggt gagtcagctc aacatcgcca tgaatgttta
    34801 gtttactgaa catacgatag cggtagctgt tcaccgtttt cggactgaga ttgagctgtt
    34861 ctgagatctc attgaccttc tggcccttgg tgatcatcag cataatctgc aattcacgtt
    34921 cagacaaact ggcaaatggg ctttctgttt tttctggttc gatctggctt aacgccattt
    34981 gttgagcgat gtcagaagca atgtaacgct gccctgaata gacagaacgg atcgcactca
    35041 cgacttcctg cggagccgcg cctttgctga ggtagccagc agcaccggcc tgcatgactt
    35101 tcgctggtaa agggttttct gtatggacgg taagcatgat gattttgaca tcagctgtgg
    35161 aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc cggcatactc atgtccatta
    35221 gcaccacgtc aacggcattt gcccggcacc atttaacggc gtcttcaccg cacgatgcct
    35281 caccgacgac ttttataccc tttatatctt ccagaatgcg tcgtatccct gcgcgcacca
    35341 gttcgtggtc atcaacaagt agaacgttga tcaaaggaat atctccagaa atagggataa
    35401 cgctactgat agttagtcat tcgtatatta gcggttttta ttgcaacttt gaaacgttaa
    35461 aaatggtggg tattcgattt ttctctcgtt tttgtcaatt cgattgtcca cagttatggt
    35521 cacgcccgtc accaacggct tccgggatcg tttcacagta agccctacga tgaaatgtga
    35581 caaaaattga ctttattcag caaaaatgaa aatcagcccg ctgaatatgt aacattaatt
    35641 aacccgaaca atacgggcaa aaaacattca tgataaaaat atttattggt cattatatta
    35701 acgtatttta tagcactgcc gatatcacgc tcaaaaaaca accactgcta tttttagcaa
    35761 agcttatggt atactccgcc gccttaacat ttttcaccgc aaattttcat tgcaacatga
    35821 cgaggaaaat aaatgagtac gcctgatttt tctactgccg agaataatca agaactggca
    35881 aatgaagtct cctgcctgaa agcgatgctg acgctgatgc tgcaggcgat gggacaagct
    35941 gacgcgggcc gcgtgatgtt aaagatggaa aaacagcttg cgctgatcga agacgaaacc
    36001 caggctgcag tattttccaa aacggttaag caaattaaac aagcctaccg tcagtaatat
    36061 aaaaccggct gagagcgtgc ctttcagccg gtttttgcat ctggcacgca gaaagaaaag
    36121 cgatcaaatt aagccagtag cggccgcgta acaggccacc tgggtcttat ttggtgcatt
    36181 gatttttttc tgcatgtttt tctgatggaa attgaccgta ttctcagaga ttgacaaaat
    36241 catcgctatc tctgctgatg tcttcccttc cgccgtccat ttcagaattt ctttttcgcg
    36301 cttgctgaaa ttcatctctg gcgtcatcac tatttcatca tttaaacgca tcagagccat
    36361 cagactttct cgcaccagta actgcatttt taattgcagt tcatcactaa gaatgggtat
    36421 ttcgcgcgtg ctgcaacggg aaaaggacaa aaagcccagc gcccgatttg gcagcattaa
    36481 atactgagtg acaccgcggc gtaaaccatg tgcgcgcgcg gcttcccata acggctgtgc
    36541 ttcgctgaat aagtcatcat tccacattaa atggccctga ctaaagtttt cagggttcag
    36601 cacaggatca attgcgagaa agttttttgc ctgataataa ctaacccaag cctcagggta
    36661 attggtgtag aaagccactt taggtcgcgt gaatggtacc gggtggcgaa cacataacga
    36721 atagtaatcg tactccagct gctgagcctg aagctctatt tcatggtaga cctcttctgc
    36781 ggcctccatc ctctgaaaac gcaacagcat cgtgcgacgc cagctgaaaa aatccgtatc
    36841 ctgcatagta aaccgcaacg cccctgagag tgagtatcat ttataatgat aattcaaact
    36901 atcacataaa taagatttat atataattta tattattcag gcaatgaatt acttttgcaa
    36961 gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca cttttattac
    37021 gcttgaaatg gcgtcccgcc ccgacaggtg aatcgtcggg gctgattttt tcttattatt
    37081 gcagcagaaa cttctcgagg aactggcggg tgcgaggctg ctgaggatcg gcaaataacg
    37141 cttttgcggc cccctgctcg actatccgcc cctggtccat aaagatcgcc cggtccgcaa
    37201 catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca
    37261 gctggcggat ggtgttcagg acttcgccca ccagctctgg atccagagcc gacgttggct
    37321 cgtcaaacaa aatcacctca ggacgcatag ccagcgctcg cgcaatcgcg acacgctgct
    37381 gttgaccgcc agacaaacga cgtggatagc tggtttcttt acctgccagc ccaactttcg
    37441 ccagcagctc gcgagcgcgc gccgtggcct cttcttttgg ttcgcctttg acgatcaccg
    37501 gcccttcaat aatgttttcc agcaccgtac gatgcggaaa caaattaaag ttctggaaga
    37561 caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt aatgaacgtg
    37621 cagtatcaat ggtgatatcg ccaacggtga tggttcccgc ttccggttgt tccagcagat
    37681 ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataatt gccaccactt
    37741 cgccaggctt cacctcaagg tcgataccgt gcagcaccgt ctgaccgtgg aattttttca
    37801 ccaggttttt aacttcaatg gcactcattt tggttctctc tcctggcgat taagttgatt
    37861 ctcaaaatgg ttctgcaacg tcgataatac tgtcgccatg atccagtaga tgagcgaagc
    37921 cgccaggtac atggtgaaca cttccagcgt gcgcgaggta atcaactgcg cctgacggaa
    37981 cagctccggc acctggattg tcgcggccag cgacgtatct tttaccaggc tgataaagct
    38041 gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatggt
    38101 ctgccacggc gtcataccaa tactggcagc agcttcccac tgacctttat caatagaaga
    38161 aatggcggcg cgcagcgttt cggcagcata ggcggcagtg ttcagcgaca gaccaatcat
    38221 tgctgacgga atgggatcta gttcgatgcc aaactgcggc aggccgtaat agatcataaa
    38281 cagttgtgcg atgagtggcg taccacgaaa aatagagata taaaaacgcg ccagccagcg
    38341 cacgggccag attggcgaaa ggcgcatcag cgcgagaata aaccctagca gtaagccaaa
    38401 aaacatgccg ccgatactga gttgcagcgt atacccagcc cctttcagca ggaacggcaa
    38461 ggaatcaata accagttgta tactttcttg catgagcgtt tccggagcta aacgtgggga
    38521 tgataggcga acagcgcagg cgcgccgccg gtatgaataa acagaatcgg cccttcatct
    38581 ttgaagcgtt tctgactgat accgtcaatc agccccgcca tcgcttttcc ggtatacaca
    38641 ggatcaagca gaatgccttc cagccgcgcc agcaatttca ctgcttccat gccttcgtcg
    38701 ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc cgctgatgcg
    38761 gtcagctcca gttctttcgc aatcgcctgt tggaggttaa ccactttcgg caattgatcg
    38821 gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc
    38881 ccaacagcca gtccggcgtg agttccggca ctgcccgatg caaccaccac cgacgaaata
    38941 tgaactgccc cttcacactg ttgcgcaatt tccagcgcac tctccacata acccagcgcg
    39001 cccaaagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttctact
    39061 cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcgtcgca catttcaatc
    39121 tgggtattga acagatccag caacaaacga ttgccgttgg ttaaatagtt ttctgcggtt
    39181 gtgccaatag gattttccag cagcgccacg cagtgcagac cgagtttcgc agcgactgcg
    39241 gcagtctggc gcacatggtt agactggatc gccccggcag taatcagcgt atcggcacct
    39301 tcacgcagag catctgccgc gagaaattcc agcttacgta atttattgcc gcccattgcc
    39361 atgggagtga cgtcatcccg tttgatgaaa atttcccgtc caagataatc agaaaagcgc
    39421 ggcagatatt cgagcggcgt tggcgcgccg ataaactcca gccgtggaaa acgggttaaa
    39481 ttatgcagtg gcataacagc ctccgatgtg tgttgttgtg attttcttat tatgcacgct
    39541 gaaaacgcgt aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat tatgctgatt
    39601 atttggtcac atcagcacca aaccattttt cggaaagggc ttgcagagtg ccatcttttt
    39661 gcatttccgc aattgcatca ttcaccgctt tcagcaggtc ttcatttcct ttacgcagcg
    39721 ccacaccaga ctcctgacgg gagaatgctt caccggttac tgccagcgta tcgttggttt
    39781 tcttcaccag atccagcgcc gccagacggt caacgaggat cgcatcgata cggcctacgc
    39841 gcagatcctg atatttggtc gggtcatcat cataggtacg cacatcgacg ccctgaacat
    39901 tctgacgcag ccactcttca tagttggtgc ccagaccgac acccactttt ttgcctttca
    39961 gatcatcagc cgttttaatg gtgccttcgt tacctttttt caccagcgcc tgaataccgg
    40021 aaatggtgta cggggtagag aaatcgtatt ttttcttgcg ctcgtcagaa atggtgacct
    40081 gattaatcac cacatcaatg cgtttagagt ccagcgacgc cagcataccg tcccatttgg
    40141 tcggttttag tgacgcctca acgccaagat gttttgccag ctgttgggca aattccactt
    40201 caaaaccggt taatttgccg tcatccccct gaaaactgaa cggcggataa gttccttcca
    40261 gccctaccag cagcgtgccg cgctctttga ctttattaag cagaccttca tctgcaaaac
    40321 ttttaacgct catgcccgca accagcgcca cggccatcac acccatcaat gcctgacgtc
    40381 ccagatgtgc taatttcata ttcaccccga atgttgttat gtctgtttgc agtgtagagc
    40441 cattgtttgt aaacacaaaa acaactccgc tacatcttat tcttatttaa tatatatcag
    40501 aagcaggcag gctggaggat ttctgcacaa gtccggtaca cgtttgacgc tgatagtgtt
    40561 gatacttccg taacgcaatg cggtaattgt tggtgctggt ggtgggcagc cacggggcga
    40621 ggatttcatc aaggaaaccg tcctgcagct gatcgaggga aatattttgc tcgtgtagat
    40681 gattacccaa acggcgcaga cgaacgacat attcacgcac cgtacctggg ctcatttcag
    40741 tttgctcaaa aagaaattgc ttaaaaccga taatatcgaa aaaatcactc tgcgttttgc
    40801 aatgtaaatc accgcaaaat cggcacaatg ccgcccatga tttttgttcc gtttgccagc
    40861 cattttcatc aagaagcatg tccaggcggg aaatctcgat tttattcact attttgccgt
    40921 cgcgaacgag ggtaatgcgg tcgagtaatt tacggcaatg cgcgcaatgg gtctggctgt
    40981 gtttaaagtc tttaagatag cggcttaatg gccgtctttt caggtgctgc accatcatta
    41041 agaactcctg gtagtcaaaa ttaaagtgcg gcatttactg acgttataac ttacccagtt
    41101 tagtgcgtaa ccgtttaatg gcctggctgt gtaactgact gacccgcgat tccccgacct
    41161 ccagcaccgc gccaatctct ttgagattca gctcttcctg gtaatagagg gttaatacca
    41221 gtttttcgcg ctccggcaac gtttcgatag cttccatcac ccgctggcgc agattactgt
    41281 ccagcagttg ttgtagcggg ttttctcgct gatgatcatc agtaaccagt tcgatgctat
    41341 cgccgtgctc ttcgcgccac tcatcgtagg agaagagctg gctgttattg gtgtcgagca
    41401 acatttggcg atagtcggca atatcgatcc ctaaacgttc cgctacctca ggttccgtgg
    41461 cgttgcggcc aagttcctgc tccagttgcc ctattgcctg tgccacttca cgcgcgttgc
    41521 gtcgcacgct gcgcggcacc cagtcacggc tgcgaagttc atccagcata gcgccacgga
    41581 tacgctgcac tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag cgttcgacgg
    41641 cattgagtaa cccaatgccg cccgcctgta gcagatcgtc aagttccacg ctcgcgggca
    41701 gtcgaacctg caggcgcaat gcttcgtgac gcaccagcgg gacataacgc tgccacagcg
    41761 agtgtttatc cattacacct tcagcggtat agagtgaatt cacgataaac agccctgcgt
    41821 tatatgagtt atcggcatga ttatccgttt ctgcagggtt tttaatcgga cgattagtgg
    41881 gtgaaatgag gggttatttg ggggttacgc aaaaagccaa ttgtaaaaaa ccccgccgga
    41941 gcggggtttg agaggcattc aggcgattaa ccctgcagca gagacagaac ctgctgcggt
    42001 acctggttgg cttttgccag cacggagttg cctgcctgct ggatgatctg cgctttcgac
    42061 atattggaca cttcggtcgc atagtcggcg tcctgaatac gggactgcgc ttcggacagg
    42121 ttggtagtgg tgttgttcag gttggtgact gcggaatcca gacggttttg aacggcaccg
    42181 agggaggagc ggaatttgtc gatctggctg atagcgtcgt caagagctgc cagcggatct
    42241 gatgttgatt ctgctttgct agtcgtatcc gttgttaact taccatcgga acctttgtag
    42301 accagtttac cggcagccgc atcctcagta gttgtgatac ttccatcagc ttgtgcatag
    42361 taagttggcg gagtatcact gagaacccca ttgctcatat aagcggtgtc tgcagcagca
    42421 gaacctgttg ttacgctata agtcttagca cctacagtgg cagtataagt gtcatttttt
    42481 actgcatttg caaatgtatt ggcatcaatg gacattgatg caccatcaaa cgttgtaccg
    42541 tcactggtga atgttccttt atcagtttgg atggtcgcgg ctttagcacc agtagcagta
    42601 gcagtagcaa gtttagccaa agtagcttgt gtatcaccac cagcattatt tttagttaag
    42661 ttaccggtag catccatata aagtactgcg ccatcatcag cagcagttaa attaccggat
    42721 ttatcgatgt ttacatcttg atctttgcca ccaattgtaa ctgtagcttt agtcgtatct
    42781 ccggcctgag gattgaggaa agcagccaga ttagcgctgg aaatatcatc agaagcggcg
    42841 gaatatgaat tagtgtcttt attgtaggta taggttacag cagcggctgg tgtaccaaaa
    42901 ccaacatcgg cgctagttgt aactttattt ccatctgtgc cagcaacagc atttagaata
    42961 tctacgctac ttgctacagc cgatttgtca gtatattgag ttacaccaac agttccccct
    43021 ggtgccgccg cagctgtgaa tccagacata tctttcaggg ttgctgccgt attagccgtt
    43081 tcccccttgc cgttcacatt aaacccattc agccccagcg tatcagagtc aattttcttc
    43141 agatcaatag taatcgtttc gccatcgttc gcgccgacct gaattttcat cgagccatct
    43201 tttgacagta cgttcacgcc gttgaactgg gtctggccgg atacgcggtc aatttcatca
    43261 agacgggatt tgatttcgtc ctgaatggag tccaggtcag aggtggagtt agttccggtc
    43321 gtcgcctgaa ccgtcagttc acgaatacgc tgtaagttgt tgttgatttc ggacagcgcg
    43381 ccttcggtgg tctgtgcaac agaaataccg tcgttggcgt tacgtgcagc ctgagtcagg
    43441 cctttaatat tagaagtaaa acggttagca atcgcctgac ctgcggcgtc atccttcgcg
    43501 ctgttaatac gcaagccaga agacagacgc tcgatagaac tcgacagcgc agactggttc
    43561 ttgttgatat tattttgagt gatcagcgag aggctgttgg tattaatgac ttgtgccatg
    43621 attcgttatc ctatattgca agtcgttgat tacgtattgg gtttccaccc gtcggctcaa
    43681 tcgccgtcaa ccctgttatc gtctgtcgta aaacaacctt tagaattttt ttcacaaaca
    43741 gccatttttt gttagtcgcc gaaatactct tttctctgcc ccttattccc gctattaaaa
    43801 aaaacaatta aacgtaaact ttgcgcaatt caggccgata accccggtat tcgttttacg
    43861 tgtcgaaaga taaaaggaaa tcgcatggca agtatttcat cgctgggagt cgggtcaggc
    43921 ctggatttaa gttcaatcct tgatagcctc accgccgcgc aaaaagcgac gctaacccct
    43981 atttcaaatc agcaatcgtc gtttaccgct aaacttagcg cctacggtac gctgaaaagc
    44041 gcgctgacga ctttccagac cgccaatact gcattgtcta aagccgatct tttttccgcc
    44101 accagcacta ccagcagcac caccgcgttc agtgccacca ccgcgggtaa cgccatcgcc
    44161 gggaaataca ccatcagcgt cacccatctg gcgcaggcgc aaaccctgac cacgcgcacc
    44221 accagagacg atacgaaaac ggcgatcgcc accagcgaca gcaaactcac cattcaacaa
    44281 ggcggcgaca aagatccgat taccattgat atcagcgcgg ctaactcgtc gttaagcggg
    44341 atccgtgatg ccatcaacaa cgcaaaagcg ggcgtaagtg cgagcatcat taacgtgggt
    44401 aacggtgaat atcgtctgtc agtcacatca aatgacaccg gccttgataa tgcgatgaca
    44461 ctctcggtca gcggtgatga tgcgctacaa agttttatgg gctatgacgc cagtgccagc
    44521 aacaacggca tggaggtctc ggtcgccgcc cagaatgcgc agctgacggt caacaacgtc
    44581 gccattgaga acagcagcaa caccatcagc gacgcgctgg aaaacatcac cctgaacctg
    44641 aacgatgtca ccacgggcaa ccagacgcta accatcactc aggacacctc caaagcgcaa
    44701 acggcgatta aagactgggt gaatgcctat aactcgctaa tagatacctt cagcagcctg
    44761 accaaataca ccgccgtaga tgcgggagct gatagccaga gttctagcaa tggcgcactg
    44821 ctcggcgact ccacgctgcg gacgattcag acgcagttga aatcgatgct gagtaatacc
    44881 gtcagttctt ccaactataa aacgctggcg cagattggta tcacgaccga tcccagcgat
    44941 ggcaaactgg aactggatgc cgacaaactc accgctgcac taaaaaaaga tgccagcggc
    45001 gtaggtgcat tgattgttgg cgatggtaaa aaaaccggca tcacgaccac catcggcagc
    45061 aacctgacca gttggctttc gacaacaggc attattaaag ccgctaccga tggcgttagt
    45121 aagaccctga ataagttaac taaagactac aacgccgcca gcgatcgcat tgatgcgcag
    45181 gtcgcgcgct ataaagaaca atttacccaa ctggacgttt taatgacctc gttaaatagc
    45241 accagcagct acttaacgca gcagttcgaa aacaacagta attccaagta atcaatattc
    45301 atcgggagac aggtcatgta cgcggcaaaa ggcacccagg cctatgcaca aattggcgtc
    45361 gaaagcgccg taatgagcgc cagccagcag cagctggtca ccatgctatt tgatggagtg
    45421 ctaagcgcac tggttagagc gcgcctgttt atgcaggaca acaatcagca aggcaaaggc
    45481 gtctctttgt caaaagcgat caacatcatt gagaacgggc tgcgggtgag tcttgatgaa
    45541 gagagcaaag acgaactaac ccaaaacttg attgctcttt atagctatat ggtcaggcgt
    45601 ttgctgcaag ccaatttacg caacgatgtc tccgcagtcg aagaagtgga agcattaatg
    45661 cgcaatattg ccgatgcctg gaaagagtct ttactctccc cttctttgat tcaggaccca
    45721 gtctgatgaa caatgcaccg catttgtatt tcgcctggca acaactcgtc gaaaaaagcc
    45781 agctcatgtt acgcctggca acggaagaac aatgggacga actcatcgcc agcgaaatgg
    45841 cgtatgtgaa tgcggtgcag gagattgcac atctgactga agaggttgcc ccgtccacca
    45901 cgatgcagga gcagctccgc ccgatgctgc gcctgattct cgacaacgaa agcaaggtaa
    45961 agcagttatt acagattcgg atggatgaac tggcgaaact ggtcggtcag tcatcggtgc
    46021 aaaaatcagt gttaagtgcc tatggcgatc agggcggctt tgtgctggct ccgcaagata
    46081 acctcttttg aatcttaacg agccgatggc tcgcgaataa tccgattacg gctacgcttc
    46141 taatgttccc cttgaatgga gtcgaagaat gcgtaatccc acgctgttac aatgttttca
    46201 ctggtattac ccggaaggcg gtaagctctg gcctgaactg gccgagcgcg cagacggttt
    46261 taatgatatt ggtatcaata tggtctggtt gccgcccgcc tataaaggcg catcgggcgg
    46321 ctattcggtc ggctacgact cctatgattt atttgatcta ggtgagtttg atcagaaagg
    46381 cagcatccct actaaatatg gtgataaagc acaactgctg gccgccattg acgccctgaa
    46441 acgtaatgac attgcggtgc tgctggacgt ggtagtcaac cacaaaatgg gcgcggatga
    46501 aaaagaagct attcgcgtgc agcgtgtaaa tgctgatgac cgtacgcaaa ttgacgaaga
    46561 aatcattgag tgtgaaggct ggacgcgtta caccttcccc gctcgtgccg ggcaatactc
    46621 gcagtttatc tgggatttca aatgttttag cggtatcgac catatcgaaa accctgatga
    46681 agatggcatt tttaaaattg ttaacgacta taccggagaa ggctggaacg atcaggttga
    46741 tgatgaactc ggtaatttcg attatctgat gggggagaat atcgattttc gcaatcatgc
    46801 cgtgacggaa gagattaaat actgggcgcg ctgggtgatg gaacaaacgc aatgcgacgg
    46861 ctttcgtctt gatgcggtca aacatattcc ggcatggttt tataaagagt ggatcgaaca
    46921 cgtacaggaa gttgcgccaa agccgttgtt tattgtggct gaatactggt cgcatgaagt
    46981 cgataaactg caaacgtata ttgatcaggt ggaaggcaaa accatgctgt ttgatgcgcc
    47041 gctgcagatg aaattccatg aagcatcgcg catgggtcgc gactacgaca tgacgcagat
    47101 cttcactggg acgctggtgg aagccgatcc ttttcacgcc gtaaccctcg ttgccaacca
    47161 cgacacccaa ccgttgcaag ccctcgaagc ccccgtcgaa ccgtggttta aaccgctggc
    47221 gtatgcctta attttgttgc gggaaaatgg cgttccttcg gtgttctatc cggacctcta
    47281 cggtgcacat tacgaagatg tcggtggtga cgggcaaacc tatccgatag atatgccaat
    47341 aatcgaacag cttgatgagt taattctcgc ccgtcagcgt ttcgcccacg gtgtacagac
    47401 gttatttttc gaccatccga actgcattgc ctttagccgc agtggcaccg acgaatatcc
    47461 cggctgcgtg gtggtcatgt cgaacggaga tgatggcgaa aaaaccattc atctgggaga
    47521 gaattacggc aataaaacct ggcgtgattt tctggggaac cggcaagaga gcgtagtgac
    47581 agacgaaaac ggcgaagcga ccttcttttg caacggcggc agcgtcagcg tgtgggttat
    47641 cgaagaggtg atttaaatcc atccccggcg gcaagccggg gagatttcat tacggcagtg
    47701 gcgtcggtaa cggcgcttta tccagcgcgg cggcacactc tgccgtcggg cgatccacac
    47761 gctccatcgt cataccatcg tactcaatgg tgttaccgtc acgctcaacc tcgtacagct
    47821 cacgttttac ggtaacgtta gtgagatcat cagacatcaa tgtcaactta cccggcacgg
    47881 caatcacccg ctgccactga cggcaatcca gggtatcgcc ctctttagtg acaatgaggc
    47941 tgcctatcgc ttccggactg accaacgcac gttgcggccc tttggtctgc cagtagcccg
    48001 ccagccagtc cggcgcgggg gttttcacga cattgttata gttttcgact tcggcacagc
    48061 ccgccagcaa catgagcgca cctgcaattg cgagtttttt catcattcgt cctgcatgcg
    48121 aagaaaagag gattgtggca ttaaagccct gatgtcgcca gcctttccag gttcagagag
    48181 aaatttgatc taaccattat ttttgtgaat tattaccccg aaaataaatt tgcatggtgt
    48241 atgattcgcg aatcttgttt caaaccttct gagttcagag gctacattca tgtcatggca
    48301 gcaattcaaa cacgcctggt tgattaaatt ctgggcgccc atccccgcgg tcatcgcggc
    48361 gggtattctc tccacttact attttggcat cactggcacc ttttgggctg tcacaggtga
    48421 atttacccgt tggggcggtc agctcctgca gttgttcggc gtccatgctg aagagtgggg
    48481 ttattttaaa attatccatc tggaaggatc gccattaacc cgcatcgacg gaatgatgat
    48541 cctcggtatg tttggcggct gttttgccgc agcgctgtgg gccaacaatg tcaaactgcg
    48601 catgccgcgc agccgtatcc gcattatgca ggccatcatt ggcggtatta tcgccggttt
    48661 tggcgcacgt ctggcaatgg gctgtaacct ggcggcgttc tttaccggaa ttccacagtt
    48721 ctcgctgcac gcctggttct ttgccatcgc cactgccatt ggttcatggt ttggcgcgcg
    48781 ctttaccctt ctgcccatct tccgtattcc cgtgaaaatg cagaaagttt ctgctgcctc
    48841 accgttgacg caaaaaccgg atcaggcgcg gcgtcgtttt cgtctcggga tgctggtctt
    48901 tttcggcctg ctgggctggg cgctgctgac ggcgatgaac cagcccaaac tggggctggc
    48961 aatgctgttt ggtgtcggct ttggtttact gattgaacgt gcgcaaatct gctttacttc
    49021 ggcgttccgc gatatgtgga tcaccggacg tacccatatg gcgaaagcaa tcattatcgg
    49081 tatggcggta agtgccatcg ggatcttcag ttacgtacag ttaggcgttg aacccaaaat
    49141 catgtgggcg ggaccaaacg cggtaattgg tggtttactg tttggttttg gcatcgtgct
    49201 ggcaggcggc tgcgaaaccg gctggatgta ccgcgcggta gaaggccagg tgcactactg
    49261 gtgggtcggt ctgggcaacg tgatcggctc aacgattctg gcgtactact gggatgattt
    49321 cgctccggcg ctggccaccg actgggacaa aatcaacctg ctgaaaacct ttggcccgat
    49381 gggcggcctg ctggtgacat atttgctgtt gtttgctgct ctaatgttga ttattggctg
    49441 ggaaaaacgc ttcttccgcc gtgcggcacc gcagactgct aaggagatcg catgaaaaat
    49501 atcgttcctg attaccgtct tgatatggtg ggtgaaccct gcccttatcc ggcagtcgca
    49561 acccttgagg cgatgccgca gttaaaaaaa ggggagatcc tggaagtggt gagcgactgt
    49621 ccgcagtcga tcaataatat tccactggat gcgcgtaatc acggctatac ggtgctggat
    49681 attcagcaag acgggccgac cattcgttat ttaattcaga agtaatccct cattcccgct
    49741 gtggcccaca gcgggaatat cgttgcctct ctatcacata gatttaaccc gccccctctc
    49801 ctattctttt cagtgtgacc tcaggaggtt tctatgtgtg gacgctttgc ccaatcccaa
    49861 acgcgtgaag attaccttgc gcttctcgcg gaagatattg aacgcgatat tccctacgat
    49921 cccgaaccta ttggcagata caacgtcgcg ccgggaacca aagtccttct gctcagtgaa
    49981 cgtgatgaac accttcatct ggatccggtt ttctggggat atgctcccgg atggtgggat
    50041 aaaccgccgc tgattaacgc ccgcgtagaa actgccgcaa ccagtcacat gtttaaaccg
    50101 ctctggcaac atggtcgggc aatctgtttt gccgatggct ggtttgagtg gaaaaaagaa
    50161 ggcgacaaaa agcagcctta ttttatctat cgtgctgatg gacaagctgt ttttatagcc
    50221 gcgataggta gcacaccgtt tgagcgtggt gacgaagccg gaagaagttt ttgtaagccc
    50281 gctcagggtc tttcagtgac tgttgcaatg gctgtgaggg cgaatcttta agccattgcg
    50341 tttcagtggc atttttccac tcaacca
//
LOCUS       DAPOZO010000027        49389 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPOZO010000027 DAPOZO010000000
VERSION     DAPOZO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.027"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            903..2120
                     /gene="alaA"
                     /locus_tag="ROI21_001708"
     CDS             903..2120
                     /gene="alaA"
                     /locus_tag="ROI21_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDX5110636.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSSYHQL"
     gene            2204..2803
                     /gene="yfbR"
                     /locus_tag="ROI21_001709"
     CDS             2204..2803
                     /gene="yfbR"
                     /locus_tag="ROI21_001709"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDX5110637.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2862..4694)
                     /locus_tag="ROI21_001710"
     CDS             complement(2862..4694)
                     /locus_tag="ROI21_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDX5110638.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGEAMEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4781..5431)
                     /gene="hxpA"
                     /locus_tag="ROI21_001711"
     CDS             complement(4781..5431)
                     /gene="hxpA"
                     /locus_tag="ROI21_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDX5110639.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKVAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(5442..5936)
                     /locus_tag="ROI21_001712"
     CDS             complement(5442..5936)
                     /locus_tag="ROI21_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDX5110640.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLKTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(6019..6474)
                     /locus_tag="ROI21_001713"
     CDS             complement(6019..6474)
                     /locus_tag="ROI21_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDX5110641.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6812..8014
                     /gene="ackA"
                     /locus_tag="ROI21_001714"
     CDS             6812..8014
                     /gene="ackA"
                     /locus_tag="ROI21_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDX5110642.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            8089..10233
                     /gene="pta"
                     /locus_tag="ROI21_001715"
     CDS             8089..10233
                     /gene="pta"
                     /locus_tag="ROI21_001715"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDX5110643.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            10423..11943
                     /gene="yfcC"
                     /locus_tag="ROI21_001716"
     CDS             10423..11943
                     /gene="yfcC"
                     /locus_tag="ROI21_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDX5110644.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11976..12518)
                     /gene="yfcD"
                     /locus_tag="ROI21_001717"
     CDS             complement(11976..12518)
                     /gene="yfcD"
                     /locus_tag="ROI21_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDX5110645.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12576..13127)
                     /gene="yfcE"
                     /locus_tag="ROI21_001718"
     CDS             complement(12576..13127)
                     /gene="yfcE"
                     /locus_tag="ROI21_001718"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDX5110646.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(13183..13827)
                     /gene="yfcF"
                     /locus_tag="ROI21_001719"
     CDS             complement(13183..13827)
                     /gene="yfcF"
                     /locus_tag="ROI21_001719"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDX5110647.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGRPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13966..14613
                     /gene="yfcG"
                     /locus_tag="ROI21_001720"
     CDS             13966..14613
                     /gene="yfcG"
                     /locus_tag="ROI21_001720"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDX5110648.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWSRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14670..15032
                     /gene="folX"
                     /locus_tag="ROI21_001721"
     CDS             14670..15032
                     /gene="folX"
                     /locus_tag="ROI21_001721"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDX5110649.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            15053..15946
                     /locus_tag="ROI21_001722"
     CDS             15053..15946
                     /locus_tag="ROI21_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDX5110650.1"
                     /translation="MKIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADHSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15994..16896)
                     /gene="rpnB"
                     /locus_tag="ROI21_001723"
     CDS             complement(15994..16896)
                     /gene="rpnB"
                     /locus_tag="ROI21_001723"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDX5110651.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARQIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVLMVTRLSPDDLIAQSH"
     gene            complement(17093..17866)
                     /gene="hisP"
                     /locus_tag="ROI21_001724"
     CDS             complement(17093..17866)
                     /gene="hisP"
                     /locus_tag="ROI21_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDX5110652.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17874..18590)
                     /locus_tag="ROI21_001725"
     CDS             complement(17874..18590)
                     /locus_tag="ROI21_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5110653.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18587..19273)
                     /gene="hisQ"
                     /locus_tag="ROI21_001726"
     CDS             complement(18587..19273)
                     /gene="hisQ"
                     /locus_tag="ROI21_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDX5110654.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLVLERR
                     YSVGVKRADL"
     gene            complement(19363..20145)
                     /gene="hisJ"
                     /locus_tag="ROI21_001727"
     CDS             complement(19363..20145)
                     /gene="hisJ"
                     /locus_tag="ROI21_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDX5110655.1"
                     /translation="MKKLVLSLSLVLAFSSATVAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(20366..21148)
                     /gene="argT"
                     /locus_tag="ROI21_001728"
     CDS             complement(20366..21148)
                     /gene="argT"
                     /locus_tag="ROI21_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDX5110656.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(21414..21983)
                     /gene="ubiX"
                     /locus_tag="ROI21_001729"
     CDS             complement(21414..21983)
                     /gene="ubiX"
                     /locus_tag="ROI21_001729"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDX5110657.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVADVETHLVMSQAARQTL
                     SLETDFSLREVQASADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(22078..23595)
                     /gene="purF"
                     /locus_tag="ROI21_001730"
     CDS             complement(22078..23595)
                     /gene="purF"
                     /locus_tag="ROI21_001730"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDX5110658.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23632..24120)
                     /gene="cvpA"
                     /locus_tag="ROI21_001731"
     CDS             complement(23632..24120)
                     /gene="cvpA"
                     /locus_tag="ROI21_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDX5110659.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(24379..25029)
                     /gene="dedD"
                     /locus_tag="ROI21_001732"
     CDS             complement(24379..25029)
                     /gene="dedD"
                     /locus_tag="ROI21_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDX5110660.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPVVEEKAAPTGKAYVVQLGALKNADKVNEIVGKL
                     RGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYTPN"
     gene            complement(25019..26287)
                     /gene="folC"
                     /locus_tag="ROI21_001733"
     CDS             complement(25019..26287)
                     /gene="folC"
                     /locus_tag="ROI21_001733"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDX5110661.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIANAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(26357..27271)
                     /gene="accD"
                     /locus_tag="ROI21_001734"
     CDS             complement(26357..27271)
                     /gene="accD"
                     /locus_tag="ROI21_001734"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDX5110662.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(27427..28086)
                     /locus_tag="ROI21_001735"
     CDS             complement(27427..28086)
                     /locus_tag="ROI21_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDX5110663.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(28169..28981)
                     /gene="truA"
                     /locus_tag="ROI21_001736"
     CDS             complement(28169..28981)
                     /gene="truA"
                     /locus_tag="ROI21_001736"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDX5110664.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28981..29994)
                     /locus_tag="ROI21_001737"
     CDS             complement(28981..29994)
                     /locus_tag="ROI21_001737"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDX5110665.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(30060..31196)
                     /gene="pdxB"
                     /locus_tag="ROI21_001738"
     CDS             complement(30060..31196)
                     /gene="pdxB"
                     /locus_tag="ROI21_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDX5110666.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLRERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            31295..32290
                     /gene="flk"
                     /locus_tag="ROI21_001739"
     CDS             31295..32290
                     /gene="flk"
                     /locus_tag="ROI21_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDX5110667.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQSLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(32287..33465)
                     /locus_tag="ROI21_001740"
     CDS             complement(32287..33465)
                     /locus_tag="ROI21_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HDX5110668.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33741..34961)
                     /gene="fabB"
                     /locus_tag="ROI21_001741"
     CDS             complement(33741..34961)
                     /gene="fabB"
                     /locus_tag="ROI21_001741"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDX5110669.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            35120..37126
                     /gene="mnmC"
                     /locus_tag="ROI21_001742"
     CDS             35120..37126
                     /gene="mnmC"
                     /locus_tag="ROI21_001742"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDX5110670.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSKLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     QFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVT
                     TNCSGITYPEGGWLCPAELTRNVLELAQQQGLQIHYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHNVVVLANGHQISGFSQTSTLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSNEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(37182..37460)
                     /locus_tag="ROI21_001743"
     CDS             complement(37182..37460)
                     /locus_tag="ROI21_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDX5110671.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37494..38042)
                     /gene="epmC"
                     /locus_tag="ROI21_001744"
     CDS             complement(37494..38042)
                     /gene="epmC"
                     /locus_tag="ROI21_001744"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDX5110672.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLENGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(38042..38851)
                     /locus_tag="ROI21_001745"
     CDS             complement(38042..38851)
                     /locus_tag="ROI21_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDX5110673.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGIN"
     gene            complement(38851..39675)
                     /gene="mepA"
                     /locus_tag="ROI21_001746"
     CDS             complement(38851..39675)
                     /gene="mepA"
                     /locus_tag="ROI21_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDX5110674.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLSLGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39679..40764)
                     /gene="aroC"
                     /locus_tag="ROI21_001747"
     CDS             complement(39679..40764)
                     /gene="aroC"
                     /locus_tag="ROI21_001747"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDX5110675.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40799..41731)
                     /gene="prmB"
                     /locus_tag="ROI21_001748"
     CDS             complement(40799..41731)
                     /gene="prmB"
                     /locus_tag="ROI21_001748"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDX5110676.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            41897..42448
                     /gene="smrB"
                     /locus_tag="ROI21_001749"
     CDS             41897..42448
                     /gene="smrB"
                     /locus_tag="ROI21_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDX5110677.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELDALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVDEWLPPELP"
     gene            complement(42518..43381)
                     /locus_tag="ROI21_001750"
     CDS             complement(42518..43381)
                     /locus_tag="ROI21_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDX5110678.1"
                     /translation="MKIMRWILGLLISLFFSTAVQANVIVATMYTPIGYASYSTKITY
                     YLDVLTPDSVNHGVYETPNNTGLITGWIPLKSWTGPGPAPSLKVISMTAISQSSCPGL
                     AEYDSRAQRTMWTCYEIRMEVWKEDSSVHGCPWLVSTHADSIDLIDPAPGTYSGRTVS
                     NSSCPPIPLGPYDVSWNESRVVHDKTLALQSTGGIIEKTLSTYLMKDGKLCDGSKFGD
                     TDDRGAYCRWVSQMLTFTSSGCDNAKVTVTPNRHPITDKELHDMVLRVDTTSRQPIDS
                     TCRFQYVLNML"
     gene            complement(43383..43928)
                     /locus_tag="ROI21_001751"
     CDS             complement(43383..43928)
                     /locus_tag="ROI21_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5110679.1"
                     /translation="MNKLKKYQCIRVQTFLLGGVLVLASHSALAANNLHFYGNLMSKT
                     CTPVEQAGTLAEVQFANISHKDLMVRGQSDRVPVVFRLKDCRGPAQYSVKVTLTGTED
                     SGQPGFLALDDTSGAQGVGIGMETTDGTAVAINNTTGVTFVLNDGNNSLNFRAWLQAK
                     EGRDVTPGNFTAQLTATFEYI"
     gene            complement(43925..44404)
                     /locus_tag="ROI21_001752"
     CDS             complement(43925..44404)
                     /locus_tag="ROI21_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5110680.1"
                     /translation="MKRLSLILLSAFCTITHAAPEDITFTGTLIEPPVCTVSNGDDIE
                     IQFIDVIIDNIDGVNYRKDVPYQITCDPDITDDAWVMTLTWTGTQTNYNDAAIQTDVT
                     GLGIELQQNGQPFKLNTPLNVTPANLPKLKAAPVKASDATLTDGNFSAYATLQVDYQ"
     gene            complement(44401..44892)
                     /locus_tag="ROI21_001753"
     CDS             complement(44401..44892)
                     /locus_tag="ROI21_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5110681.1"
                     /translation="MKRLLLAGLGGVLLTGSALAGETKSVPLTLRVLVDPPPPCTVKG
                     SNVDFGDVIIKKINGTEYRKDAGYTLNCANSLSDDLQMQFKGTTTTINGETVLSTGIT
                     GFGIRIENAADNSLFAIGDNTWTPFSVSSQPQLKAVPVKQNGVTLTGAQFNASMTMVV
                     DYQ"
     gene            complement(44908..45657)
                     /locus_tag="ROI21_001754"
     CDS             complement(44908..45657)
                     /locus_tag="ROI21_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDX5110682.1"
                     /translation="MTKYLSAKTLTTVLSLLAATVSFSTQASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKLPYLAQSWIEDEKGVKISSPLAVLPPVQRIEAMMNGQVKIQGLPDIH
                     KLPADRESMFYFNVREIPPKSNKSNTLQIALQTRIKLFWRPKALEKNTTQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPVL
                     TYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(45677..48316)
                     /locus_tag="ROI21_001755"
     CDS             complement(45677..48316)
                     /locus_tag="ROI21_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX5110683.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNALADDTIQFDGRFLDLKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNQPLPDDYDIYWYATENDPNKSYACLSPELV
                     AQFGLKEDIAKNLQWIRDGQCLNTALLAGTEISGDLGQSALLVSVPQAYLEYTDSEWD
                     PPSRWDDGIPGLIADYSINAQTRHENGGDDTNDICGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNLRGYAPDISGVAHTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALLGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDDDRARTGNDKEMY
                     TVTYNQNFTDARVSVYLNYSHHTYWDRQDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSNQVSLYHRIDDASHYTLSAGTS
                     ENHSSVDGYYSHDGTLAKVDLSANYHEGQYTSAGISLQGGATLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATK
                     SVVQATLTEGAIGYRKFAVISGEKAMAVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSSPVIPHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            complement(48400..48966)
                     /locus_tag="ROI21_001756"
     CDS             complement(48400..48966)
                     /locus_tag="ROI21_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5110684.1"
                     /translation="MSKFVKAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCS
                     IVPDDHKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMATTFTGNPYSTN
                     ADNYSLSNMDNGTEIPNVSLVIGDQHGTGYALGAEIKQPIVKDSSTGKGKPKQTLNFK
                     AWLVGETDAVTPTPAPFETLTTFQITYL"
BASE COUNT        12223 a        13200 c        12191 g        11775 t
ORIGIN      
        1 cggctcatac tgagctggtt aatgctgtgc acacacaaac aagcaattcc atttgtaatg
       61 tgcctccctg gcagtttcat ccggaacccg gacgaaagta aaaatgcata tgagttgcac
      121 taaaaaagcg actcacattg ttccgttata atgcctgaag tagatcacag aatatatctt
      181 caggaatcgc atatctatta agttactcac tcttttgtat ttatgacatg cgcgtgtttg
      241 tataaatgta aatgtgagtc cttgttccac tctcgtgcag catcgctggt catacgcgaa
      301 cacgtacaaa cagcaatggt gaggcggcat caagagcagg gatccgtcat ttatgcgagc
      361 attttacccc aaaaaccttt tatttataag ggtcattgcg aattatctga tgcaaagtta
      421 tgttatgtta ggcaaagtaa tcttctatta ttaataagca caacaaaacc tttttgaata
      481 ttaaataata attaattagc atcatcctca tacattaatc ccgcttaaca atagtttcac
      541 accttgcacc cattacgaca cctcggaaat catcacgctg ttaaactctc cgtaatgcgg
      601 aaatatcatc atcacgattc attagtgaat tcttaaacga ggcattttca aacaattatc
      661 ttacagataa aaaaaccaca ctcacaatta agattcagaa caattcacta cataacatta
      721 attactaagc atatacacct cattattttg ccattattaa gttattaaca gcacaatcga
      781 gtcttcccct ctggcaaaat cttattctgc agaccttcaa aacgccgtcc tgggggagta
      841 cattgttcta agctgacttc cacggcaggg agtggcgata acagcaaaaa aggtcaagat
      901 tcatgtcccc cattgaaaaa tccagcaaat tagagaatgt ctgttacgac atccgtggtc
      961 cggtgctgaa agaagcaaaa cgcctggaag aagaaggtaa caaggtactg aaactgaaca
     1021 tcggcaaccc tgccccgttc ggttttgacg cgccagatga aatcctcgtt gacgtgattc
     1081 gcaacctgcc taccgctcaa gggtattgcg attccaaagg gctttactcc gcgcgtaaag
     1141 ccatcatgca gcactaccag gctcgtggca tgcgtgatgt taccgtggaa gatatttaca
     1201 tcggcaatgg tgtatcggag cttatcgttc aggcaatgca ggcattgctg aacagcggcg
     1261 acgaaatgtt ggttcctgca cctgattacc cactttggac cgcggcggtt tcgctctcca
     1321 gcggtaaagc ggtgcattat ctttgcgatg aatcctctga ctggttcccg gaccttgatg
     1381 atattcgcgc taaaattacg cctcgtacgc gtgggatcgt tattatcaac ccaaataacc
     1441 caactggcgc ggtatattcc aaagagcttt taatggagat agtggagatt gcacgtcagc
     1501 ataatctcat tatcttcgcc gatgaaattt atgacaaaat tctctacgac gacgctgagc
     1561 atcactcaat tgcgccgctg gcacctgatc tgctgactat tacctttaac ggactgtcga
     1621 aaacgtaccg cgttgctggc ttccgtcagg ggtggatggt gttgaacggg ccgaaaaaac
     1681 acgccaaagg ttacatcgaa ggtctggaaa tgctggcctc aatgcgcctg tgtgctaacg
     1741 ttcctgcgca acacgccatt cagactgcac taggcggtta tcagagcatc agtgaattta
     1801 ttactcccgg cgggcgtctt tatgagcagc gtaaccgcgc gtgggaattg atcaacgata
     1861 ttccgggcgt ttcctgcgtg aaaccgcgcg gtgcgctgta catgttcccg aaaatcgacg
     1921 ccaaacgctt taatattcac gacgatcaga aaatggtgct ggatttcctg ttgcaggaaa
     1981 aagttctgtt ggtgcaaggg acggcattca actggccgtg gccggatcac ttccgcattg
     2041 tcacgctacc gcgtgtcgat gatatcgagc tgtctttgag caagttcgcg cgtttccttt
     2101 ctagttatca tcagctgtaa tcttaatttc actgccggag attgcatccg gcagtgttat
     2161 cccgccacaa tgacctgatg atgtcatcat acgtaaggtc actatgaaac agagccattt
     2221 ttttgcccat ctctcccgcc tgaaactcat taaccgctgg ccgctgatgc gtaacgtgcg
     2281 gactgaaaat gtgtccgaac acagtttgca ggtagcgatg gtcgcccatg cgctggcagc
     2341 tatcaaaaat cggaaatttg gcggtaatgt caacgccgaa cgtatcgctt tactggcgat
     2401 gtaccacgat gcctcagaag tgctcaccgg cgatctccct actccggtga aatacttcaa
     2461 ttcgcaaatc gctcaggagt acaaggcgat tgaaaaaatc gctcagcaaa aactggtcga
     2521 tatggttccg gaagagctgc gggatatctt tgcgccgtta attgacgagc atgcatatag
     2581 cgatgaagaa aaatcgctgg tgaaacaggc agatgcactg tgtgcatatc tgaaatgtct
     2641 ggaagaactc gcggcgggaa ataatgaatt cttgctggca aaaacgcgac tggaagcgac
     2701 gcttgaagcg cgccgcagcc aggagatgga ctacttcatg gaagtctttg ttcccagctt
     2761 ccatctttcg ctcgatgaga ttagccagga ttcaccgctg taagcggccg gagtctgcgt
     2821 cgcatcaggc aataagcgcc ggatgcgaca tcaggctctt gtcaaaacgg aaacagcatc
     2881 gggatcatca ccacacaaac cgccatcacg ataatggtga acggtacccc caacttcaca
     2941 aagtcactga agctgtaatt tcccggacct aaaaccagtg tgttaacagg tgaagaaacc
     3001 ggcgtcataa aggcggcgga tgctgccatc gccacgacca tcgcaaatgg ataaggcgac
     3061 acacccatcg ttttggcagc agccagcgca atcggagcca tcaacaccgc cgttgcggta
     3121 ttagagataa atagcccaat aaccgccgac aagacaaaca aacaacccag catcatatgt
     3181 ggcccataac cgccgccaat gtccatcagc cctttcaccg ccagcgcgac acctcccgtt
     3241 ttctgtaatg ccacagcaaa tggcatcatc ccaacgatca aaataatgct cggccagtga
     3301 atggatttat aggcgctttc agcatcgata cagcggaatt tccccatcag caggcaggcg
     3361 atgatagcgg caacaggatt agggatttca tcggtcagca ttaacgccac catcaacacc
     3421 aggcaaaaaa tggcatgagg tgcctgactg tgtgctggcg atgcttcact cacttcttct
     3481 ggtaagttca gcgccacaaa gtcacgcccc tgttttgcca gcataccgat cagtttccag
     3541 ttacccacaa ccaggatgat atcgcccagc agcagaggct catccgccag cgaaccttcc
     3601 atcgcctcgc cattgcgctt tagccccacc acattcagtc cgtagcgggt acgaaaacca
     3661 atttcgcgca ccgatttacc aataagttca gactcaggaa ttaatgaaat ctctgccatg
     3721 cccacatcaa gtgcctggtc agaaaaatac tcgccgcgta gtaccatcgg ctccagcaac
     3781 tgctcactac aaaattgccg gagatcgaca tcagccgcag acatatcaat aagcaaaacg
     3841 tcacgcgcgc gaaattcaga aaccccatta acgttcacga taacgcgacg aaaacgccgc
     3901 cagcgttcaa caccgatgac gttagcgcca taacgctcac gtaatttgag atcgtccagc
     3961 cgttgaccaa tcattggcga tccggggcga atagccagac gtcgcgcacg cccggtcagt
     4021 cgatattcac ggataagatc gcgaaaggtt cgacgcgtcc agccttcgcg ctgcggggtc
     4081 tgggtatccc ctttcagcat gaaacgcatc actaacatat acaaaatacc cagcaccagc
     4141 acgaccaggc caataggtgt tacgctaaag aaactgaagc cgtgatagcc ttcacgcagc
     4201 aattcactgt tgactaccag gttcggcggc gtcgccacca gcgtcatcat gccgcttatc
     4261 agcccggcaa aacttaacgg catcatcaga cgcgacggcg acgtttgcat acgcatggca
     4321 acgcttaaca ccacgggaat aaagatagcg acaacgccgg ttgaactcat aaacgcgcca
     4381 agccccgcga cggtcagcat caacaaaacc aacattttga tttcactgtt gcccgccact
     4441 ttgaccagcc atgtccccat tacagtggca acaccagtac ggaccaaacc atcgccaata
     4501 ataaacaagg cggcaatcag gacaacgtta ggatcggaaa agccggaaaa tacttcgggg
     4561 accgtcagcg ttccgcttaa tgcaaacgcg acaataacaa acaaagcgac cgcatccata
     4621 cgcactctgc ccgtcgcaaa caagacgatg gcaacggcca gtaatgaaag aacccaaatc
     4681 aattcaccgt tcacaactta tccttgttaa ttgaggggga tgacttgatt ctgccataaa
     4741 aaagccccga cgagacgggg ctaaatcatg atcaggtgtt tcactgaata ataacatcac
     4801 catttggctg tttggtcaca gtaatttgct ccagactgtg gaggaccaaa tcgacctcat
     4861 tcaggcgcgg ggtatctgcc ggagcgttaa ccgcaatgac atgacaaccc gccgccaggc
     4921 cagaaagcac gccagcggga gcatcttcca ccaccacaca ctcctgcggc gcaagcccca
     4981 gcagctgcgc gcctaacaga tacgcatcag gttctggttt tccgcgcttc actcgctcag
     5041 cggttacaaa cacctctggt gcgggaagcc cagctacttt atggcgcgct cgcgctaccg
     5101 gcatggagcc agaagtcaca atggcccacg gaatacctgc tttattcaaa tgattgagta
     5161 aggcgattgc ccccggaagc gcggtaatac cctcggtttc cgtggcctcg atttgctcca
     5221 gacgcgtaaa ctcggcggca atatcagcct cggatttgcc cgccataaaa tggcgcagag
     5281 aggtgatcgc ctgtttaccg tgaatgaaag ccagcacctc ttccggcgct aacccatgac
     5341 gtctggccca gttgctccac gcccgctcta ccgcaggcag ggaatccacc agcgttccat
     5401 caagatcaaa cagaaaacct ttgcaccgca cgcggacctc ctcaggcgtt gataatttga
     5461 ttaatttcgt tggcgctcaa atggtactga cgcgggcagg catgccacac attaagcatg
     5521 cgctgatatt tttcccacat tggcgtctgg gcgttaaaac cgtgagttcc ggcgtcaaaa
     5581 tgggtatagc gcccttccac attaaccata aagcggacat aaccgaggta acgtgcttca
     5641 gtggcggcgt caaagccgag gaaggtaaca cgacgttcat cgatggattg ctggtcctgc
     5701 aaattagacc aggaaacatg caacgcatga tacatctcca taatgtcgat gatagtgcgg
     5761 caggtttctt ctttcagctc gccaaactcg cgatccagtt cacgcatctg taatccataa
     5821 ccacgctcaa tgattgtttt caggcgacgg taacgttcag catttgccgg atcgagcata
     5881 gtcatcatct tgtactggtt agacaaaata agacgttgcg cgttggtcat ttccattgtt
     5941 gactcctgta tcactctact acggtgaaaa aaaagaaggc tgagtatgcc ttcttttata
     6001 tgcgtaatca gggggcaatt acaaatcatc aaggaaagtt ttatccagtt gtttgaaggc
     6061 gcgcttaagc gtgtcagcta atgcctggta atcaggcttg ccttcaacgg gtgccaacac
     6121 ctgtccagac tcctgcaatt taccgcgaac ttcataaaac cagttgagga tagcaggggg
     6181 taatggcgtt acagaacgct tgcccagcca ccacaatccc tgcatgggta aacttaaggc
     6241 gaacagcgca gtggcaactg ccggcccaag ctgaccgccc agggcaatct gccagcagag
     6301 agtaaatacg gcgatcggcg gcataaaacg gatcgcataa cgcgtcatct tgataacgcg
     6361 attttcgaca aagaccgggg caaggcgttt ttccagcggc cacgtctttg agtaatgctg
     6421 tccccggcga aacaagctaa aaaaattaac agaacgatta tccggcgttg acatgcttca
     6481 cctcaacttc acatataaag attcaaaaat ttgtgcaaat tcacaactca gcgggacaac
     6541 gttcaaaaca ttttgtcttc catacccact atcaggtatc ctttagcagc ctgaaggcct
     6601 aagtagtaca tattcattga gtcgtcaaat tcatatacat tatgccattg gctgaaaatt
     6661 acgcaaaatg gcatagactc aagatatttc ttccatcatg caaaaaaaat ttgcagtgca
     6721 tgatgttaat cataaatgtc ggtgtcatca tgcgctacgc tctatggctc cctgacgttt
     6781 ttttagccac gtatcaatta taggtacttc catgtcgagt aagttagtac tggttctgaa
     6841 ctgcggtagt tcttcactga aatttgccat catcgatgca gtaaatggtg aagagtacct
     6901 ttctggttta gccgaatgtt tccacctgcc cgaagcacgt atcaaatgga aaatggacgg
     6961 caataaacag gaagcggctt taggtgcagg cgccgctcac agcgaagcgc tcaactttat
     7021 cgttaatact attctggcac aaaaaccaga actgtctgcg cagctgactg ctatcggtca
     7081 ccgtatcgta cacggcggcg aaaagtatac cagctccgta gtgatcgatg agtctgttat
     7141 tcagggtatc aaagatgcag cttcttttgc accgctgcac aacccggctc acctgatcgg
     7201 tatcgaagaa gctctgaaat ctttcccaca gctgaaagac aaaaacgttg ctgtatttga
     7261 caccgcgttc caccagacta tgccggaaga gtcttacctc tacgccctgc cgtacaacct
     7321 gtacaaagag cacggcatcc gtcgttacgg cgcgcacggc accagccact tctatgtaac
     7381 ccaggaagcg gcaaaaatgc tgaacaaacc ggtagaagaa ctgaacatca tcacctgcca
     7441 cctgggcaac ggtggttccg tttctgctat ccgcaacggt aaatgcgttg acacctctat
     7501 gggcctgacc ccgctggaag gtctggtcat gggtacccgt tctggtgata tcgatccggc
     7561 gatcatcttc cacctgcacg acaccctggg catgagcgtt gacgcaatca acaaactgct
     7621 gaccaaagag tctggcctgc tgggtctgac cgaagtgacc agcgactgcc gctatgttga
     7681 agacaactac gcgacgaaag aagacgcgaa gcgcgcaatg gacgtttact gccaccgcct
     7741 ggcgaaatac atcggtgcct acactgcgct gatggatggt cgtctggacg ctgttgtatt
     7801 caccggtggt atcggtgaaa atgccgcgat ggttcgtgag ctgtctctgg gcaaactggg
     7861 cgtgctgggc tttgaagttg atcatgaacg caacctggct gcacgtttcg gcaaatctgg
     7921 tttcatcaac aaagaaggta cccgtcctgc ggtggttatc ccaaccaacg aagaactggt
     7981 tatcgcgcaa gacgcgagcc gcctgactgc ctgatttcac accgccagct aagctggcgg
     8041 tgctgttttg taacccgcca aatcggcggt aacgaaagag gataaaccgt gtcccgtatt
     8101 attatgctga tccctaccgg aaccagcgtc ggtctgacca gcgtcagcct tggcgtgatc
     8161 cgtgcaatgg aacgcaaagg cgttcgtctg agcgttttca aacctatcgc tcagccgcgt
     8221 accggtggcg atgcgcccga tcagactacc actatcgtgc gtgcgaactc ttccaccacg
     8281 acggccgctg aaccgctgaa aatgagctac gttgaaggtc tgctttccag caatcagaaa
     8341 gatgtgctga tggaagagat cgtcgcaaac taccacgcta acaccaaaga cgctgaagtc
     8401 gttctggttg aaggtctggt cccgacacgt aagcaccagt ttgcccagtc tctgaactac
     8461 gaaatcgcta aaacgctgaa tgcggaaatc gtcttcgtta tgtctcaggg cactgacacc
     8521 ccggaacagc tgaaagagcg tatcgaactg acccgcaaca gcttcggcgg tgccaaaaac
     8581 accaacatca ccggcgttat cgttaacaaa ctgaacgcac cggttgatga acagggtcgt
     8641 actcgcccgg atctgtccga gattttcgac gactcttcca aagctaaagt aaacaatgtt
     8701 gatccggcga agctgcaaga atccagcccg ctgccggttc tcggcgctgt gccgtggagc
     8761 tttgacctga tcgcgactcg tgcgatcgat atggctcgcc acctgaatgc gaccatcatc
     8821 aacgaaggcg acatcaatac tcgccgcgtt aaatccgtca ctttctgcgc acgcagcatt
     8881 ccacacatgc tggagcactt ccgtgccggt tctctgctgg tgacttccgc agaccgtcct
     8941 gacgtgctgg ttgccgcgtg cctggctgcc atgaacggcg tagaaatcgg tgccctgctg
     9001 ctgactggcg gctacgaaat ggacgcgcgc atttctaaac tgtgcgaacg tgctttcgct
     9061 accggcctgc cggtatttat ggtgaacacc aacacctggc agacttctct gagcctgcag
     9121 agcttcaacc tggaagttcc ggttgacgat cacgagcgta tcgagaaagt tcaggaatac
     9181 gttgctaact acatcaacgc tgactggatc gattctctga ctgccacttc tgagcgcagc
     9241 cgtcgtctgt ctccgccagc gttccgttat cagctgactg aacttgcgcg caaagcgggc
     9301 aaacgtatcg ttctgccgga aggtgacgaa ccgcgtaccg ttaaggcagc cgctatctgt
     9361 gctgaacgtg gtatcgcaac ttgcgtactg ctgggtaatc cggcagagat caaccgtgtt
     9421 gcagcgtctc agggtgtaga actgggtgca ggcattgaaa tcgttgatcc agaagtggtt
     9481 cgcgaaagct atgttggtcg tctggtcgaa ctgcgtaaga acaaaggcat gaccgaaacc
     9541 gttgcccgcg aacagctgga agataacgta gttctcggta cgctgatgct ggaacaggat
     9601 gaagttgatg gtctggtttc cggtgctgtt cacactaccg caaacaccat ccgtccgccg
     9661 ctgcagctga tcaaaactgc accgggcagc tccctggtat cttccgtgtt cttcatgctg
     9721 ctgccggaac aggtttacgt ttacggtgac tgtgcgatca acccggatcc gactgcagaa
     9781 cagctggcag aaatcgcgat tcagtccgct gattccgctg cggccttcgg tatcgaaccg
     9841 cgcgttgcta tgctctccta ctccaccggg acttctggtg ctggtagcga cgtagaaaaa
     9901 gttcgcgaag caactcgtct ggcgcaggaa aaacgtcctg acctgatgat cgacggtccg
     9961 ctgcagtacg acgctgcggt aatggctgac gttgcgaaat ccaaagcgcc gaactctccg
    10021 gttgcaggtc gcgctaccgt gttcatcttc ccggatctga acaccggtaa caccacctac
    10081 aaagcagtac agcgttctgc cgatctgatc tccatcgggc cgatgctgca gggtatgcgc
    10141 aagccggtta acgacctgtc ccgtggcgca ctggttgacg atatcgtcta caccatcgcg
    10201 ctgactgcga ttcagtctgc acagcagcag taatctcgtc atcatccgca gctttgcgct
    10261 gcggatatct gaaccggaaa tagtcactat ttccggtttt ttattctctt aatttgcatt
    10321 aatcctttct gattatcttg cttagctgcg ctgcatcaat gaattgcgcc atctcacttt
    10381 gcatacttac cactttgttt tgtgcaaggg aatatttgcg ctatgtccgc aatcactgaa
    10441 tctaaaccaa caagaagatg ggcaatgccc gatacgttgg tgattatctt ttttgttgct
    10501 attttaacca gccttgccac ctgggtagtt ccggtgggga tgtttgacag tcaggaagtg
    10561 cagtatcagg ttgatggtca aacaaaaact cgcaaagtcg tagatccaca ctcttttcgc
    10621 attctgacta acgaagcagg cgaacctgag tatcaccgcg tacagctgtt cacgacgggc
    10681 gatgaacgcc cgggtctgat gaacttcccg tttgaagggt taacctcagg atcgaaatac
    10741 gggacagccg ttggcatcat catgtttatg ctggtgattg gcggcgcgtt tggcattgtg
    10801 atgcgtacag gaaccattga taacggtatc ctggcgctta ttcgccatac ccgcggaaat
    10861 gaaattctct ttattcctgc gctctttatt ctgttttcac ttggcggcgc ggtatttggt
    10921 atgggagaag aggccgtcgc ctttgccatt atcatcgcac cgctaatggt ccggctaggc
    10981 tatgacagta ttaccaccgt cctggtgacc tatatcgcca cgcaaatcgg ttttgccagt
    11041 tcgtggatga acccgttttg tgtggttgtt gctcagggga ttgccggcgt tccggtgctt
    11101 tctggctccg ggttgcgcat cgtggtgtgg gttatcgcca ctctgattgg cctgatcttt
    11161 accatggtgt acgcctcacg agtgaaaaag aatcctcttc tgtcacgcgt gcatgagtcc
    11221 gaccgcttct ttcgtgaaaa gcaggcggat gttgaacaac gtccgtttac ctttggtgac
    11281 tggctggtat tgattgtcct gaccgccgta atggtctggg tgatttgggg cgtgatcgtt
    11341 aatgcctggt ttattccaga aattgccagc cagttcttca ccatgggtct ggtgattggc
    11401 atcatcggcg ttgttttccg ccttaacggc atgacggtta ataccatggc ttcatccttc
    11461 accgaagggg cgcgaatgat gatcgcccct gccctgctgg taggtttcgc caaagggatt
    11521 ttgctgctgg tcggtaatgg tgaagcgggt gatgccagcg tgttaaatac catcctcaac
    11581 agcattgcca atgccattag cggtctggat aacgcggtcg cggcctggtt tatgttgctc
    11641 ttccaggcgg tatttaattt cttcgtgacg tccggttctg gtcaggcggc gttaaccatg
    11701 ccgttactgg caccgcttgg cgatctggtc ggtgttaacc gtcaggttac cgtgctggcg
    11761 ttccagtttg gtgatggctt cagccacatc atttacccga cctcagcttc gttaatggca
    11821 acgctaggtg tttgccgggt ggacttccgt aactggctga aggtgggcgc gacactgctt
    11881 ggactgctgt ttattatgtc cagcgtcgtg gtgatcggcg ctcagttgat gggctaccac
    11941 taaaaatgtt aagagccgca attgcggctc ttttttcatt ctgccgtttc agtctctacg
    12001 gcttcatttt tggcattgcg cttcatccac aacgccagcg ctttcagcga gtctggagtg
    12061 aactcatcgc agcgtgcggt gatttcttcc ggtgtcagcc agcaaacttc actgacttca
    12121 tcttcctgta gggcgaaggg accgtgagag acgcaactga acaacgcgcc ccagacacgg
    12181 caatttttat cttcgaaata gaactgcccg tgctcggcaa aggggacacc ggcaatgccc
    12241 aactcttctt ccgcttcgcg acgcgcggat tccagcagtt gctcatcggc ctggactaca
    12301 ccgcctgcgg tcgcatctaa catgccgggt aaaaagtctt ttgtctcggt acgacgctgg
    12361 accagaattt tgcccatgcc atcatgcacg acgatgtaag ttgcacgatg acgcagacac
    12421 tgtgcccgca tttgttcccg gctggcttgt gcaatgactt cgttctcttc attgacaata
    12481 tccacccatt cagtacttgc caaacgacgc tgttccatta tcaggaaacc ttctttttct
    12541 ggcgctctta cggcgcattt tgagttgtgg gtaaattacg gattaatcgc gacctgcgca
    12601 atgatacttt gatcattgag tgcgattaca ctaagtacgt cattatccag catgccataa
    12661 ctcgccggat taccgccttt cggaatactc accgagccgg ggttaaagtg gaaaatctcc
    12721 ccccgctgtt ctgccactgg cagatgggta tgaccgtaca ccagcacatc gttctggttt
    12781 aaagcaggca gattttccgg cccaaacaga tgaccatgcg tcaaaaacag acgttgtttt
    12841 tccagtaata cctgttgcca cggcgcggtt atcgggaaat gcaaaagcat ttgatccact
    12901 tcgctgtcgc agttgccgcg cacagcgata accttatgtg ccacttcatt aagccgttca
    12961 gcgactttgg ctggcgcgta accctccggt aaagcattac gcgggccgtg attcaacacg
    13021 tcgccaagga tcaccagcca ctgggcaccg ctttgggcaa acaactccag aacacgttcc
    13081 gtcgccggta acgacccatg aatgtccgat gcaaacatca gtttcatcac tcactcctcg
    13141 tcgaaaaaac gtaagctatg atactggatt ctgtcgttgc tatcagccag attgcttcgc
    13201 cgaaagagca ataaaacgct ggacagacgc tcgctgccac tggaatgtcg cataatccac
    13261 caggcgttct ggcacctcat cgccatgtag taccaggcgg ttaatcatta gcgccagatc
    13321 agtatcagca atgcaccatt caccaaataa attcggccgc cccagtgcta acaaatgttc
    13381 tgccatcgcg aacagtttct ctgcactggc ttttccctcc gccgttaatg gcgctttttt
    13441 cgcacccgca aagacaacat ccgttggacg ttcttcgcgg atgggcatca gatcgctacg
    13501 cagccaggcc tgaatctgtc gtgcacgcgc acgattttct aaatcaagcg gataaatgcg
    13561 ttcccaggtc ggtggcgcaa atcgatcttc cagatactcc gcaatggcag aagattcact
    13621 caactcaaaa tcatcgattt gtaataacgg cacacggcgt gtctgaccgt aaccttgcca
    13681 cgtcggctgc aaatgctcac cgctgtcgag gtcgatggtc ttgatatgaa acgacaggcc
    13741 tttttcctgc aacgccaccc aggcggataa cacataaggg gagaaaaaat gggcatctga
    13801 ccaaagcgtg atagcgggtt tactcattat gtcctcggca ataaaggggg gggctagcta
    13861 tcaaactata gagccttttt gtcactgtca cgtgacgaat actcacgcaa gtcgccgcgc
    13921 tttctatact tgtggtgttc agatgattag acaaccggag ctgcaatgat cgatctctat
    13981 ttcgccccga caccaaatgg tcacaaaatt acgctgtttc tcgaagaagc agagctggat
    14041 tatcgcttga taaaggtaga cctggggaaa ggcggtcagt ttcgcccgga atttttgcgc
    14101 atttcgccta acaacaaaat tccggcaatt gttgatcatt ctccagccga tggcggcgaa
    14161 ccgctaagcc tctttgagtc tggtgccatt ttgttgtatc tggctgagaa aacaggactc
    14221 tttttgagtc atgaaacgcg tgagcgcgcc gccacattac agtggttatt ctggcaggta
    14281 ggcggactgg ggccgatgct tgggcaaaat catcatttta atcacgcagc cccccaaacc
    14341 attccttacg ctattgaacg ttatcaggtt gaaactcagc gtctgtacca tgtactgaac
    14401 aagcggctgg aaaactcgcc ctggctgggt ggcgagaact acagcattgc ggatattgcc
    14461 tgctggccgt gggttaatgc ctggagtcgc cagcgaattg acctcgcaat gtatccggca
    14521 gtcaagaact ggcatgagcg gatccgttcg cgccctgcca ccgggcaggc actgctaaaa
    14581 gcacaactcg gtgatgagcg ttcggatagt taacagaaac aggttctcgt gtattatttc
    14641 attctaagta aaacaacgga gaacctgcaa tggcacaacc tgccgctatt attcgtataa
    14701 agaaccttcg tttacgtacg tttatcggaa ttaaggaaga agaaattaac aaccgtcagg
    14761 atattgttat caatgtgacg atccactacc ccgccgataa agcgcgcacc agcgaagata
    14821 tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat tattcagcat gtagagaata
    14881 accgtttctc tttgctggaa aaattaactc aggatgtgct cgatatcgca cgtgaacatc
    14941 actgggtgac gtatgctgaa gtggagatcg ataaactgca cgcgctgcgc tacgccgatt
    15001 cggtatccat gaccttaagc tggcagcgtt aaccgccata tcgggaggct gcatgaagat
    15061 agtgatcacc ggagggacgg gattaattgg tcgccatttg attccgcgtt tgctggatct
    15121 gggccatcaa atcacggtag tgacgcgtaa cccgcagaaa gccagttccg ttctcggccc
    15181 tcgggtgaca ctatggcaag ggcttgccga tcatagtaac ctcaacggcg ttgatgcggt
    15241 aatcaacctg gccggagagc cgattgctga taaacgctgg actcacgagc aaaaagagcg
    15301 tctctgccaa agccgctgga atatcacgca aaaactggtc gatttgatta atgccagcga
    15361 cacgccaccg tcggtactca tttccggctc ggcaacaggc tattatggcg acttaggtga
    15421 agtggtggtt accgaagagg aaccgccgca taacgaattt acccataaac tctgcgcccg
    15481 ctgggaagag attgcctgtc gggcgcaaag tgacaaaacg cgagtgtgcc tgctgcgtac
    15541 cggcgtagtg ctggcaccag acggtggtat tctcggtaaa atgctgccgc cgtttcgtct
    15601 tggtctgggc gggccgattg gctccggtcg gcagtatctg gcctggattc atattgatga
    15661 tatggtcaac ggcattctct ggctgctgga taacgagctg cgcgggccat ttaatatggt
    15721 ttcgccctac ccggtacgca atgaacaatt tgcccatgcg ctcggtcatg cgctgcatcg
    15781 cccggccatt ttgcgcgtcc ccgcgaccgc cattcgactg ttaatgggcg aatcttcagt
    15841 actggtatta ggtggacaac gcgcgctgcc taaacggctg gaagaagcgg gttttgcgtt
    15901 tcgctggtac gatttagaag aggcgctggc ggatgttgtt cgctgatgtg gtttactgca
    15961 aacatccgcc agttaactcc cggtgttaca gggttagtgg ctttgcgcga taagatcgtc
    16021 tggtgaaagt cgggtcacca tcagaactaa ctctcggtct aaacctctat ccagcatctc
    16081 ctgagcaata cgcagggctt cttcgcgttt gccaagaatt aatccatcat tccgcccttc
    16141 ttcacgtaat ctgtcagcaa tagtcatcag tttctccttt tcttgtggtg cgcgttccgc
    16201 tatctcacca ataaatgcac ggaaacgctg ggcatccccc gtttgtaata cgtaattaaa
    16261 cagggctttt agctgtctgt cattagtgtt ccctgtaact agcagcgaaa caatttggtc
    16321 gactaatccc aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat
    16381 tttgcgatgt tgcataatct cgtcatccgg caccacggta atatccacca gcggaaaagc
    16441 cgatgaataa atttggcggg caatcgcagg ctcggcaaat tcatcaagcc agcagagtga
    16501 atatggataa ggacttctgc aaccatgata aaacagcatc gggatcacta atggaagctc
    16561 tttatagccc gcatcaagat ggttttgcat cgccgcaatg gaatagcgca tcatgcgaaa
    16621 agccattaat tcctctggct tactttggtg ctctatcacc acataaatat aacccgctcc
    16681 ctcctgcgtt ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat
    16741 aaaactgttt ggttccagtt taagtgttgt taaatcacac agtttgcgca gcggcgcggg
    16801 aagatgaata tcaataaaat cccgcgcggt gtctggatgg cgtaaaaaag atttaaatac
    16861 cgcatcatgc ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt
    16921 aagatgacgc gacaataacc acgatttaca cccaatgcag caggcagttt ttatctttgg
    16981 acagcgaatt cagaactatt tttagcggac tgcaactttt acatttcagc ggaagcggac
    17041 tcgcaaattc cccttgaaaa tactccggta gcgttagcca ccggaggggg aattatttca
    17101 gcgatccctt aaggaactgt tgcagacgag ggctttgcgg gttaccaaat aactgttctg
    17161 gcgcgccctc ttcttctatt ttcccctgat ggaggaaaat gacgtgagta gaaacatggc
    17221 gagcaaagcc catttcgtga gtcactacca ccatggtttt cccctcttct gccaattgct
    17281 gcataatacg taacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa
    17341 acagtaaaac ttccggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac
    17401 caccggacag gtgcaccggg tatttcccct gcgcacgttc gtctatcccg actttcgcca
    17461 gatacttcac cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg
    17521 cttccatgac gttttccagc accgtcatat ggctccagag attgaagtgc tggaatacca
    17581 tcgtcaggcg tgtgcgcagt aagcgcagct gatttttatc ggcgactttg agttgaccgt
    17641 ctttgtcgcg caccagattg atcgtctggc cgttaaccac gatcgatcct tcgctcggtt
    17701 tttcgaggaa gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga
    17761 tgcttatgac atctccggca ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc
    17821 cgtagcgttt gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc
    17881 gttgaagaag gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc
    17941 aggacataag agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga
    18001 tacgtggcgg cgttaatatc gcgggctatt ttcagcagat ccggcacagt agcggtgaaa
    18061 gccagcgcag tagagtgcag catcaggatc acttcgttgc tgtaggccgg taacgcaata
    18121 cgcagcgccg atggcaaaat aatgcagcga tacattttaa aggtcgagaa gccataggct
    18181 ctggcggctt cgatttcccc atgcggcact gaacggatcg ctccggcaaa aatctcggtg
    18241 gtgtaagcac aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcgaaaa
    18301 aaggcgttaa gaaactcggt ccccttaaca atctccagcg tatacatgcc ggaatagaac
    18361 accagcaact gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga
    18421 aactggatgt atttattact ggagacacga ccaatcgcca gaaacagcgc cagaactccg
    18481 cctatcacta ccgacaaaat cagcagccac agggtgatcg ccacgccagt aaagcgataa
    18541 ccgtcggtcc acaacaacgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc
    18601 tcttcacacc cacggagtag cggcgctcaa ggaccagcag cacaccattg gaaacagtgg
    18661 tgaaaaccag gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt
    18721 tgcctgccag ctgcgtggct ttgaccacat cttccaggcc aagtaacgaa accagagcgg
    18781 tagatttgag gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg
    18841 ccggaaacat gatccgccga aacacttgcc cacgagtgaa accgaacgcc gtcgccgcct
    18901 ctatatgtcc tttcggcact gccataaaag cgccacggaa cgtttcggta aaataagcac
    18961 cgtagataaa accgagagta ataataccag cgaccatcgg atcgatatca atctgcccga
    19021 cgcccatcgc ctccgtcacc gtgtttagtg caatctgcaa accgtagaaa attagcagca
    19081 tcaacaccaa gtccggcacg cctcgaatca gcgtggtgta cccttcgaaa atcagccccg
    19141 aaagccgatt ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca
    19201 ctacagagct gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa
    19261 acccatacaa catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct
    19321 ccggttaccc gaagggagcg gactttcttt caccgatggc gattaaccac cataaacatc
    19381 aaaatcgaag tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa
    19441 ggctttgttc agtgcctcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc
    19501 tacgccaaac agtttttcat ctttaacaga cggaccaccg aatttgtaat ctttaccgac
    19561 gggttgtttg aggaaacctt cgctggctgc gacctcatcc tggaacgcgg catcaatacg
    19621 tccggcagtc aggtcagaat aaatattgtc ctgcccctga taagaaacga tttcaatgcc
    19681 ttttggtgcc cagtgttcgt taccgaacgt ctcctgggtg gtgccctgca atacgcctac
    19741 ccgtttgcct ttcagcgatt cgactgtcgg ctgaatgtcg gaatttttcg ccaccaccag
    19801 acgagaatcg gctgcgtata gtttgtcggt gaatgctatt tcttgctgac gtttttccgt
    19861 aatggaaagc gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc
    19921 atccagtggg ttttcgacaa acgtacattg cgtattgatg cgtttgcaca actcctttgc
    19981 cagatcgata tcgaagccaa ccagttcacc ttgtgaattt tttgattcaa atggcgcata
    20041 ggtcggatcg gtaccgatgc ggatgttttg cggaatcgca gcaaacgcca cagttgcgct
    20101 ggagaaggcc agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga
    20161 cagtcttttt atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc
    20221 atcaaggcaa aatatcccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta
    20281 tgcatttcaa tgcaccacga tagtgcaaca aacctgccgt gcaccatgat gatcattcca
    20341 tcaggtacag cttccctgcg acgtatcagt caccgtagac attaaagtca aaatactttt
    20401 tcgccatctt gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttaaacgccg
    20461 ccgtcagttc cgcatcatct ttacgtagcc ctacaccggt gccgtcaccg aagtattttt
    20521 tgtctttcac tgatgagcca gcaaaggcga agtctttacc agcaggttgc ttgaggaatc
    20581 cttcgctggc agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg
    20641 aatagaccaa atcctggttg gcataggcca ccacatccac acctttgcta cgccaggtct
    20701 cgttagcgta agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg
    20761 aatccagcgt tggctgaatc ggcgaacctt tggcggcaat caaacgagaa tctgcggcgt
    20821 acagcttgtc ggagaaagca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa
    20881 taatggcgtc gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa
    20941 cccaggtaca tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc
    21001 caacaaagtc acctttggca tctttcgatg agaacggcgc ataggtggta tcagttccga
    21061 tacgtaccgt ctccggtagc gccgcatagc tggaagccgc tgcggagaga ccgactaaca
    21121 aagacagagc gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat
    21181 gttgtgtatt gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catctttacg
    21241 acagcagtaa cattcaacgt taaacatgtt aataagacgt tgcattattg tcctgaagtt
    21301 gaagatagca ggtatggcgg ttgaatcgca gcgtttcgat tcatcgaaag gaacaaaatg
    21361 tcgaggattt gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg
    21421 ccctgccagc gggcaaagag atcttcagga agcgttatgg cgaattgatc gagtacacgg
    21481 ttaaccgtct gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg
    21541 ggaggcataa tcaccgcacc gatttctgct gcctgagtca ttaaacgcag atggcctaag
    21601 tgcaatggtg tttcacgtac gcagagcacc aacgggcgac gctctttcag caccacatct
    21661 gccgcacggg tcagtaagcc atcagtatag ctatggacaa tgccggacag ggttttgatt
    21721 gaacagggta aaatcaccat ccccagtgtc tggaaagaac cggaagaaat gctggcagca
    21781 atatcgcgcg catcgtgcgt cacatcggct gatgcctgca cttcgcgcag ggaaaaatcc
    21841 gtttcgaggg ataaggtctg gcgcgcggcc tggctcatca ccaaatgcgt ttcaacatct
    21901 gcgacatcgc gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg
    21961 atgcctacaa tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc
    22021 aggatttcgg cgggagttgc aagtcagggt gccagaccgg caccctgagc gaaggcatca
    22081 tccttcgtta tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc
    22141 atcattacgt aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta
    22201 gacgccgttg aacaccgaac actcaaactg ctgaatatcc gggttttcag cgcgaacggc
    22261 ttcgatcaga tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat
    22321 ttcatctact tcgcgaccgt gagcgataag ctccgtagcg ctcggcatat cgataccgta
    22381 aacgttcggg aagcgaatct ccggtgccgc agaagcgagg tacactttct tcgctccggc
    22441 ttcgcgtgcc atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac
    22501 cagcaggacg tttttatcgc ggaactcggc gcggttggcg ttcagcttac ggcgcacgga
    22561 cttacgacgc agttgctgac ccggcatgat gaaggtacgg cctacatagc ggtttttaac
    22621 gaagccctga cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacatga
    22681 ggtttccggg atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt
    22741 ctcgcccagc ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgatgaa
    22801 cgagtccggg cgagcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc
    22861 agcacattga cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc
    22921 gacgtcacgc aggaaatcaa agcctagtgt atcgagcgca acgctttcgg aagcgaccat
    22981 atattctgtg cggttctcgt caatatcacg ttttcccagt accagcggac gaataccgtt
    23041 aggatcgcgg aaagcaacca taccgtggcc gataatcatc gccacacagg catacgcgcc
    23101 gcggattaag cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta
    23161 gtggcggaag ttgtccagct cgctggcgaa gatattaagc agaatctccg agtcggaagt
    23221 ggtattgatg tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt
    23281 cagattgccg ttgtgggcaa gcgtaatgcc atacggagag ttaacgtaaa acggctgcgc
    23341 ttcagaggcg ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc
    23401 ctgcaaacgc tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg
    23461 caaacggaag cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg
    23521 ctgaagcacc gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc
    23581 gacaataccg cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct
    23641 gggcaagaaa cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa
    23701 ctgcgggatc agctgtgatt tgctccagtc ttcgcttttc gacaccccgg taaaggagtc
    23761 gagaaagaag agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc
    23821 cagcacccga tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt
    23881 cacgatagca ccaacgatca gggtagcgat aaacagtacc gcgatggcaa tcccatttcg
    23941 aaccagttcg tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc
    24001 aacaaagaaa gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgcggat
    24061 caggctaacc agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat
    24121 atgtgtccca cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga
    24181 aaacgtttgc gtagggattt ccttcccacg catcaataaa aatggcgctg aaaaaatatt
    24241 caacgccatc gactttttat gcctttgcgg catcgggcaa tgcgtgtcgg atgcggcgta
    24301 aacgccttat ccgacctacg tttctactcc tgcgtaggac tgataagacg cgccagcgtc
    24361 gcatcagata agaccgtatt aattcggcgt ataacccatt accacgccac ttaagccaga
    24421 aagttgcttc aactcaccca gcgaaccttt cagcttatct ttcgaggcat ctggcccaac
    24481 cagaatacga gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc
    24541 ggcaccgcgc agcttaccga caatctcatt cactttatcg gcatttttca gtgcgcccag
    24601 ttgtacaaca taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggcgc
    24661 aggtggtgct tcaaccttcg gtttcggcgg ctctaccggt ttcggcttcg gtgggacgac
    24721 cggtgcaggt tccggttcaa actcggtgtt attggctgca atagtggccg gatcgagcga
    24781 cggtgctgcg gcatcacctg cccgcacctc ttccgctgcg ccttccggcg gctgcgtcgg
    24841 cagcgcctgg gtggcggctg gcatcatatc aggctcatcg cgatcgcccg cttttggcac
    24901 cagcgggata gccgcgaact catcctgata atgttttttc tgcccgtcca gcagccctgg
    24961 aagtacaatc acccccagcg ccaccagcac gatcgtgccc actaaccgat tctgaaactt
    25021 acttgccacc gcttctcctc gcgtcaatca cttccatgac atgtgcgacc gtgtggaacg
    25081 aaccacacac cagcacggta tcttccgctt tagcgtccgc cattgcggca tcccatgcct
    25141 gcgccacgct atcaaatgat ttgccgttac ccaaatgctc aagcagttgt tctgccgtgg
    25201 caccgcgcgg cccttccagt ggcgcacaat accagtcatc aaccacgctt ttcaaccagg
    25261 ccagagttcc ggcaatatct ttatcatgta gcataccgat aaccgccagc acgcgcccgt
    25321 ttttcggtag cgctttcata cgcccggtga gatattccgc cgcatgtgga ttatgcgcga
    25381 catcaaaaat aacgcgtggt gactcgctca caatctggaa acgtcccggc aaaattgcgt
    25441 tggcaatccc gtcgcgaatg gcattttcac tgacctccag cccgctggca cgcagtgccg
    25501 ccagcgccgt tgcggcattc ggttgcggga caagcggcaa cggcagattt tccagcgtgc
    25561 cgtgagcatc gctaaacgcc cagtcatgat cggtgacgga atagttccac tcaacgcccc
    25621 gacgttgtaa cagtgcactt ttttcctgcg ccacatcagc aatggtagaa ggcatttccg
    25681 gctcaccgac aattgccggt ttttcgctgc ggaagatgcc tgctttctcg cgaccaatgc
    25741 tttcgcgatc tggccccagc cagtcggtat gatccagcgc aatgctggtt actaccgcaa
    25801 catcggcatc gacaatattg gttgcgtcca gacgaccgcc cagccctact tccagaatca
    25861 ccacgtcaag ttgtgcctgt ttgaacagcc acaacgccga cagcgtaccg tactcgaaat
    25921 aggtcaggga aatatcaccg cgtgccgatt caatctccgc aaaagaggcg gtgtgggccg
    25981 attccggcaa ttcctgcccc tgcacacgta cgcgctcggt ataacgcacc agatgcggtg
    26041 aactgtagac gcccactttg taccctgccg ccatcagaat cgactccagc gtacggcagg
    26101 tggtgccttt gccattcgta cccgcaacgg taaacacaaa tggcgctggt ttcagaacgc
    26161 caagacgcgc cgcgacctgg ctcacgcgct caaggccgag atcgatagtt ttactgtgca
    26221 ggttttccag ataagaaagc cacgaagcca gaggcgacgc ggcttgagga gtgcgtttga
    26281 taatcatggt atccgctgat tcgttacggt gagaatagca aaagggcaga gccagtggcc
    26341 ctgcccttat cagttatcag gcctcaggtt cctgatctgg taccggtggc accactacgc
    26401 cttcacgcgg cgcttcagga ttcggcgctg gcagattcat caacttcgcc agaatactcg
    26461 ccagtttcag gcgcatttcc ggacgacgga cgatcatgtc gatcgcgcct ttctcgatca
    26521 ggaattcact gcgctggaat cctggcggca gtttttcgcg aacggtctgt tcgataacac
    26581 gcggaccggc aaagccgatt aacgctttcg gttcagcgat gttgagatcg cccagcatgg
    26641 cgaaacttgc agaaacaccg cccatggtcg ggtcggtcag cacggagatg tacggcaagc
    26701 cgcgctcctg catttttgcc agtgccgcag aggttttcgc catctgcatc agcgacatca
    26761 gtgcttcctg catacgtgcg ccaccagagg cggagaagca gatcagtggg cagttatctt
    26821 ccagcgcctg ctcaacggca cgcacgaaac gtgcacctac cacagacccc atggaaccgc
    26881 ccataaaggc gaactcgaat gccgcagcga caaccggcat tccatacagg gtgcctttca
    26941 tcaccaccag cgcatctttt tcgccggttt ctttctgcgc agatgccaga cggtctttat
    27001 acttcttgga gtcacgaaac ttcagcacat ctttcggctc aagctcgcta cccaactcca
    27061 caaggcttcc ttcatctaac aggctatgca ggcgattacg cgctgtcata cgcatgtgat
    27121 ggtcacactt cggacagacc tcaagattac gttccagctc agcgcggtat aaaacctgac
    27181 cgcagctatc acacttagtc cacacccctt caggaatgct cgccttgcgg gtgggagtaa
    27241 tgttgctttt aattcgttca atccagctca ttagggacct ttctgtctga acctggttcg
    27301 atgccagttt tatctttggg gacgcataat gccatttttg cccccaacag accatgaatg
    27361 ttgcacatta aaacataaca gcccgaaact ttggataaaa aagtggtcga accgcggagt
    27421 tactttttat tttgcggcgc gtgccgcagc gcgtttgtgg cgaataaatt cgaaaacacc
    27481 tggcaatatt gaaacaaaaa taatgccaac aatcatcagt ttaaggttgc tctgaatgaa
    27541 ggggagcgtt ccgaagaaat agcctgcgta ggtaaaaagc agtacccaca acagtgcgcc
    27601 gatcacgtta taagcggcga aatgacggta cgacatgtgg cccattcccg caacaaacgg
    27661 ggcgaacgtt ctgacgatcg gcacaaaacg ggcgagaata atcgttttgc cgccatgttt
    27721 ctcataaaac tgatgagtct tatcaagata actgcgacga aagattttcg aatccggatt
    27781 actgaacagt ttgtcaccaa ataaccgtcc aatggtgtag ttcaccgcgt cacccacaat
    27841 cgcggcgatc agcatcagca ccaccatcat atggacgttg agatcgttgg tttccagtga
    27901 tgccaacgca ccggcaacaa acagcaatga atcacccggt aaaaacggcg ttaccaccaa
    27961 accggtttca cagaacaaaa ttaagaacaa aatggcataa acccagacgc catactccgc
    28021 gaccagttct gccagatgca cgtcaatgtg caggataaaa tcgatgagga aataaatcag
    28081 gtccatatta gcctatgcct tgtactcgtc atctttcagg ctgtaactac gttggctaca
    28141 gcctgaaatg ttccgagcat tattcttgtt agtccgccag aaatagcggg cccattggcg
    28201 gttttggaag atcataccgg tcagggtaat ccaccgcgac cagatacagc ccttccgctt
    28261 ttgccgttgc tgccgccagc gttctgtcct ttgccgccag caactctgct atccagctct
    28321 ccggctggtt gtgggcaccg acttccatca ggctgccgac aatattcctg accatatgat
    28381 gtacaaaggc attcgctttg atatctacca ccacataagg accgtgacgc gtgacattaa
    28441 tatgcataac gttgcgccac ggggttcggg attggcactg caccgcacgg aacgaggtga
    28501 aatcattctc gcccagcaag cattgcgcgg cccgatgcat ccgttcagcg tccagcggtt
    28561 cgtaaaaatg ggttacccct ttactcagta ccgccgggcg cagccgatga ttgtagatga
    28621 tgtagcgata acggcgagcc gtggcgctaa atcgggcatg aaaatcatca ggtacagctt
    28681 taacccaacg cacggcgatg tcaccaggta aattcgcatt tacgcccaac gtccacgcag
    28741 cgtctttgcg ctgcgcggtc gtttcgaaat gcacaacctg cccggtgccg tgtacacctg
    28801 cgtcagtacg cccggcgcag aagacggtga tcggttcgtt cgccacctgg gagagcgcct
    28861 tttccagctt ctcctgcaca ctgcggactt cgttctgccg ttgccagccg taatacttac
    28921 tgccgtcgta ctcaatgccc agcgcaattt tataaactgg cggttgttgc tggtcggaca
    28981 ttagtacaga tactcctgca ccagtttctc ggcgattttt actgccatca gcgcgccgcc
    29041 aaagcgaacg ttatcggcca ccgaccagaa ctgaacttgc tccggcatac cgtagtcatt
    29101 acgtacgcag ccaacggaaa gatgcggcgt accagaagca tcccccacct gagtcgggaa
    29161 ttcgttctct tcagagagca caatatcttc gccctgagca aacgcatcac gcgcttcttc
    29221 tgccgccagc ggacgcagag cttcaaagtt gaccatctgg gcatgaccgt agaataccgg
    29281 tgcctggacg acgcttgccg aaatcatcag cccttcgtcc tgcaggattt tgcgtacttc
    29341 gtcaacgata cgacgttctt cacgcacgct accttcgcta tccggcagta acggcagcat
    29401 gttgaacgcc agctgacgcc cgaagaaatc ttcttcatca atcggaatgc cgttgagcaa
    29461 tttagcactc tgccccgcta acgcatcgac cgcttttttg ccctgggcgg aggctgaaat
    29521 caggctggta acgctgatac gcgacaaacc gccctgatcg attaacggtt tcagtgccgc
    29581 cagcagctga ctggtcagac tgtctggtac ggcgatgaca ttccggttgc ggtaatcggt
    29641 cagtacaaac gggtttactt ccggcaccac cagcggtacg tcgggttcga gagcaaacaa
    29701 tccgctgctg tcgatcacca ggcaacctga gttggtcgct tcttcaaccc aggcagcggt
    29761 agcttctttg cctgcgacaa aaaacgccag ctgcgcctgc gtccagtcga attcagcggc
    29821 atcctgcacg gtgattgtct taccaccaaa gcgcagttgt tcgcctgcgc tttcgttacg
    29881 tgccagtgca taaatttccc caaccgggaa ctgacgttca gccagcgttt caagcagggc
    29941 ttcgcccaca gcgccagttg cgcccaggac ggcaatgttc cagccttcag acatggtggt
    30001 ttactccaga aatagcaaag ctccctgcca atattacagc agagagcatg aagaagagat
    30061 taacgtgccg gatgatgaac ggcgttaaaa cccagtttac acagcaatga tgccgcgctg
    30121 gcgtcatcac aaattacata cagagatgac cattcacggc gctcaagata gtttttgcgc
    30181 agcttatcga actcacccgg tattccggcg actttacgca gcggtgcgtc atcgcggcgc
    30241 acatcataca ccaaatgcac cagccttttc agcgtcggtt gatcgagcgg gccatgcagc
    30301 gtaatgcggc caaactctgg cgcgggcagc aacgtatcca gcgcaacgtg ttgttcatgc
    30361 ccaataaact tgctataagc ttcaaacact tgcgtagtac cgcgtgcttt accttccagg
    30421 gtatagcctg cgatatgcgg cgtgccgata tccacttttt tcagcaattc cacgttgagt
    30481 tccggttcgc cttcccagac atccagcact acgcttaact tctggccttc gttcagacag
    30541 gtcaataggg cagtattatc gacgactgcg ccacggcagg cgttaatcag aatcgctccg
    30601 ggcttcaggc tacggatcag tttttcatcc gccagatgta gcgttttgta cggaccatct
    30661 ttaaagagtg gcgtatggaa agtcagaata tccgcatgct gaactaattc atccagcgag
    30721 cggaaatcac cctcatcccc acggtcggcg cgaggcggat cgcaaagtaa ggttttaatc
    30781 cctaacgctt ccagtcgtgc ctgtaaacgc cgaccaacgt taccaacgcc cacgatcccc
    30841 acggtacgct cgcgcagtga aaatccatcg cgttcggcaa gcatcagcag ggaggagaaa
    30901 acatattcca ccaccgcaat cgcattacag ccaggtgccg cggaaaaacc aattcccgcc
    30961 tgctttaacc acgcttcatc gacatggtcg gtccccgcgg tggcagtgcc aacaaattta
    31021 atgggttttc ctgccagcaa agattcattc actttcgtga ccgaacgcac catcagcgca
    31081 tccgcgtctg ccagttgagc gacggggatt ggacgcccgg gaaccgcggt cacctcaccc
    31141 aaacggctaa ataagtcgcg ggcataaggc atattttcat caacaaggat tttcacgttt
    31201 gtgttacctg tatgagacga gagttaaccg gacaagtgtg ccataatctc gcggccaggc
    31261 atacttgcga agatttcagg tataaggata cgtaatgata caacctattt ccggccctcc
    31321 tcctgggcaa ccaccaggtc agggagataa cctgccgtct ggcgcgggca atcagtcttt
    31381 atccagtcag caacgtactt cgctggaaag cttaatgacg aaagtgacct cactgacgca
    31441 acagcaaaga gcagaactgt gggcgggtat caggcacgat attggtctgt cgggagattc
    31501 accgctgctt tcgcgtcact tccctgccgc tgagcacaat ctggcgcaac gtctgctggc
    31561 cgcgcaaaaa agccattctg cccgccagct tttagcgcaa ttaggggagt atttacgtct
    31621 ggggaataat cgtcaggcgg tcacggatta tatccgtcat aactttggtc agacgccgct
    31681 gaatcagctc tcaccggagc aattaaagac cattctcacc ctgttgcagg aagggaagat
    31741 ggttattccg caacctcagc agcgcgaggc gaccgaccgt ccgttattac cggcggagca
    31801 caatgcgctc aaacagctgg tgaccaaact tgcggcggca acgggagaac ccagcaagca
    31861 gatctggcaa tcgatgctgg aactttccgg ggtgaaagat ggtgagttaa ttccagcgaa
    31921 actgtttaac catctggtga cctggctgca ggcgcgtcag acgctaagcc agcaaaatac
    31981 gccgacgctg gaatcactac agatggcgct gaaacaacct ttagatgcca gtgaactggc
    32041 ggcgttatcg gcatatatcc agcaaaaata tggtctttct gcgcaatcat cgctttcttc
    32101 agcccaggcc gaggatattc ttaatcagct ttatcaacgg cgggttaaag ggattgatcc
    32161 gcgtgatatg caaccgctgc ttaatccttt tccaccgatg atggacacgt tgcaaaatat
    32221 ggcaacgcgt cccgcgctgt ggatactgtt agtcgcgatt atcctgatgc tggtctggct
    32281 ggttcgttaa ccccgacgaa acgacagtat cgtgacaata attcccagca ccgcagagat
    32341 cgccccggca agaaataccg aagagtaacc aaacgtggtc gccagcattc ccgccagcgg
    32401 cccggagacg ccgagggcga tatcctgaaa cgcggcgtaa ccgcccaatg cggtgccgcg
    32461 aacttgtgag gggacgcgtt taaccacctc cacgcccagc gcaggaaaga taagcgaaca
    32521 tccggctccg gttaacgccg cgcccgctaa tgcgacccac gcaccagggg cttgccagag
    32581 cagcaacaag cccaccgttt ctacaagcag agagacaatc gccactttca cgccgccaaa
    32641 acggtccggc atccagccaa acatgacacg catcacgaca aatgcgccgc caaacgcggt
    32701 aagagtaaag cccgccatcg cccatccttt gctggcaaag tagagcgaaa cgaaagtccc
    32761 gataaccgca aaaccaacgc cttgtagtgc cagacctaac ccaggtttcc agataagccc
    32821 gacaacactc cacagcgatg gacgttctcc cgccagggcc ggtactttgc gcactgtgcc
    32881 gttacaggcc cacgccagta agggtaatgc cattgtggtg agcgccagtg cggcaaaacc
    32941 gtaatggcta tgaatcaaca ggccaagcgg cgcaccaaca gcgagggcac cgtaaatcgc
    33001 cattccgttc catgacatca ctttgccgga atgttttggc ccaacgatgc ctaatcccca
    33061 ggtcagagcg cccgtcagta actggctttc accaaagcca agaatcaaac gcccgacgac
    33121 caacagggca aatttgaacg gtgcggagac gggcaaaatc gccgccaaca gcagcgcgcc
    33181 gccagccaga ccacaagcta acatcccctg aagcgccgaa cgttttgcgc catattgatc
    33241 ggccagtcgc ccggcatagc cacgcgtcag caccgtagcc agaaactgaa tgccgacggc
    33301 aatcccgacc atggtgttgc catagcccag ttcatgatga acaaacagcg ggataaccgg
    33361 caacggcagc cctacggtca tgtaggtgag aaaaaccgca aaagcgatgc ggaagagcga
    33421 aaaattggca gaagatcgtg tttcggtttg gcttacagca gtcatgcatt actccagaat
    33481 gcagcgcaag gcgaggagtc tccccgtctc atctctctgg ttttcagggt tacggtgcgt
    33541 tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct
    33601 ggaacaggcg gcgattgtca ataatgtgaa aaagggaacc atcaggttcc cttttgcgtt
    33661 agtgtcggat gccgcattgg cgcgtaatgt tggatgcgac gctggcgcgt cttatccgac
    33721 ctactccggc ctactgcgaa ttaatctttc agcttgcgca ttaccagcgt ggcgttggtg
    33781 ccgccgaagc cgaagctgtt agacataacg gtggtcagtt cgcgatcggt cgtttcggtc
    33841 acgatgttca gacccgcagc ctgctcgtcc agctcttcaa tgttgatgct cggggcgata
    33901 aagccgtgtt ccagcatcag cagagagtag atagcttcct gtacgccagc agcgcccaga
    33961 gagtgaccgg tcattgcttt ggttgcagaa atcgccgggc tcttatcgcc gaatacttca
    34021 cggatagccg ccagctcttt cacgtcgcca accggagtcg aagtaccgtg agagttcagg
    34081 tagtcgattg gggtatcaac accgtgcatc gccatcttca tgcagcgcac tgcgccttcg
    34141 ccagacggag caaccatgtc tgcgccatca gacgttgcgc cgtagccaac gatttcagcg
    34201 tagatatgag caccacgtgc caacgcgtgt tccagttctt cgaccaccac cataccgccg
    34261 ccgccagcga taacgaaacc gtcacggtga gcgtcataag tacgggaggc tttttccggg
    34321 gtgtcgttgt atttggtaga cagcgcaccc atcgcgtcga attcgcaagc catttcccag
    34381 cacagctctt cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg gatctgctct
    34441 actgcgttac cgatacagtg tgcggaagtc gcacacgcgg agctgatgga gtagttaacg
    34501 ccgtgaatct taaacggcgt ggcgaggcag gcagaaacgc cggatgccat cgctttggtg
    34561 accacatacg ggcctaccgc tttcaggccg cgcgggccgc gcattgcgtc agcgccgaac
    34621 acctgaaaac gcggggaacc gccgccggaa cctgcaatca ggccaacgcg cgggttattc
    34681 tggtaagctt ccggggagag gccagcatct gcaattgcct gctccataga aaggaatgca
    34741 taaatggatg cgtcgctcat aaagcgcaca actttgcggt caatgaggcc agtggtatcc
    34801 agttttacgt tgccccagac gtggctacgc atgccggaat ccttcagctc ctgagagaaa
    34861 gtgatccctg aacgtccttc acgcagagat gccaggactt cctgctggtt attaccgatg
    34921 ctggaaacaa tgcccaggcc agtaatcact gcacgtttca ttcaatacct ctgtaagtcg
    34981 cacatagagt aagtttcgaa tgcacaatag cgtacacttg tacgccgaac aagtccgatc
    35041 agccaattaa tagagaaatt tgcgcagcct gacacacatc gctaagatcg agccaccgcc
    35101 tgtaagacga gtaacttacg tgaaacacta ctccatacaa cctgccaacc tcgaatttaa
    35161 tgctgagggt acacctgttt cccgagattt tgacgatgtc tatttttcca acgataacgg
    35221 actggaagag acacgctatg tttttctcgg aggtaaccaa ttagaggcac gctttcctga
    35281 gcatccacat cctctgtttg tggtagcaga gagcggcttc ggcaccggat taaacttcct
    35341 gacgctatgg caggcatttg atcagtttcg cgaagcacat ccgcaagcgc aattacaacg
    35401 cttacatttc attagttttg agaaatttcc cctcacccgt gcggatttag ccttagcgca
    35461 tcaacactgg ccggaactgg ctccgtgggc agaacaactt caggcgcagt ggccaatgcc
    35521 cttgcccggt tgccatcgtt tattactcga tgaaggccgc gtgacgctgg atttatggtt
    35581 tggcgatatt aatgaactga ccagcaaact ggacgattcg ctaaatcaaa aagtagatgc
    35641 ctggtttctg gacggctttg cgccagcgaa aaacccggat atgtggacgc aaaatctgtt
    35701 taacgccatg gcccggctgg cgcgtccggg cggtacgctg gcgacattta cgtctgccgg
    35761 ttttgtccgc cgcggtttgc aggacgccgg attcacgatg caaaaacgta agggctttgg
    35821 gcgtaaacgg gaaatgctat gtggggtgat ggaacagaca ttaccgctcc cctgctccgc
    35881 gccgtggttt aaccgtacgg gcagcagcaa acgggaagcg gcgattatcg gcggtggtat
    35941 tgccagcgcg ttgttgtcgc tggcgctatt gcgtcgcggc tggcaggtaa cactttattg
    36001 cgcggatgag gcccccgcac tgggtgcttc cggcaatcgc cagggggcgc tgtatccgct
    36061 cttaagtaaa cacgatgagg cgctgaaccg ctttttctct aatgcgttta cttttgctcg
    36121 tcagttttac gaccaattac ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt
    36181 aggctgggat gagaaaagcc agcataaaat cgcacagatg ctgtcgatgg atttacccga
    36241 agaactggct gtagccgttg aggcaaatgc ggttgaacaa attacgggcg ttacgacaaa
    36301 ttgcagcggt attacttatc cggaaggtgg ctggctgtgt ccggcagaac taacccgtaa
    36361 tgtgctggaa ctagcgcaac agcaaggttt acagattcat tatcaatatc agttgcagga
    36421 tttatcccgt aaggatgact gttggttgct gaattttgca ggagatcagc aagcaacaca
    36481 caatgtagtg gtactggcga acgggcatca aatcagcggt ttcagccaaa catcgactct
    36541 gccagtgtat tcggttgccg gacaggtcag ccatattccg acaacgccgg aactggcaaa
    36601 actgaaacag gtgctgtgct atgacggtta tctcacgcca caaaatccgg cgaatcagca
    36661 ccattgtatt ggtgccagtt atcatcgcgg cagcgaagat acggcgtaca gtgaggacga
    36721 tcagcagcag aatcgccagc ggttgattga ttgtttcccg caggcacagt gggcaaaaga
    36781 ggttgatgtc agtgataaag aggcgcgctg cggtgtgcgt tgtgccactc gcgatcatct
    36841 gccaatggta ggcaatgttc ccgattatga ggcaacactc gtggaatatg cgtcgctggc
    36901 ggagaagaaa gatgaagcgg taagcgcgcc ggtttatgac gatctcttta tgtttgctgc
    36961 tttaggttcg cgcggtttgt gctctgcccc gctgtgtgcc gagattctgg cggcgcagat
    37021 gagcaacgaa ccgattccga tggatgccag tacgctggcg gcgttaaatc cgaatcggtt
    37081 atgggtgcgg aaattgttga agggtaaagc ggttaaggcg gggtaatctc ctttggcagt
    37141 gtttgccgga tgcggcgtga acgccttatc cggcacatta cttactgctg tgacgcctgc
    37201 tgaaacaggt tttcccacat atcggtcacc agcgcctggt cacgcggcga caactcaccg
    37261 gcaccaatgg ctttttccag actctggcta acggttgtat gtaccgcctg ggcggagtgg
    37321 tcatcaccac tttccagttc tgcgatggct aatgtcaggt ggccacgcaa atacccactg
    37381 gcaaacaact catcatcact ggcatggtca accataccgt cgattaatgc cagaatgcgt
    37441 gattcaaact ccgcgatcat cttctttcct cattgtaaaa cgtggcgctg gctttagttg
    37501 agcgcttccg gccacgggaa ctgttccgcc gtaagttccg gcgtgtgata ataattttgt
    37561 aatgctttaa tgaagcgtgc cgggcgttca ggaatgccgt tttccagata atccatcacc
    37621 tgtgcatgta cccgccgttg aaataccacg cggtccggtt cgaaatcccc ttccagattg
    37681 tcgcagctaa cattaaacgg atatcccgcc gccacacaga acaaccagtc gaacgcctgc
    37741 ggcttcactt caacatcttc aaactggctt tgcgtttggg catcgcgtcc gtccgggcaa
    37801 taccagtagc cgaaatcaac cagctcacgg cgcgctttcc cggcaataca ccagtgcgaa
    37861 atctcatgaa ttgcgctggc ataaaagcca tgagcaaaga cgattcggtt atacggtact
    37921 tccgcatcag caggaagata gatcggttcg tcgtcgcctt taatcagacg ggtattaaaa
    37981 tcatcggcaa agcagctatt aaaaatttca atcaactgct catagtggtg tgtactgttc
    38041 attagttaat ccccaaccag tggaggatct cctgtccgtg gctatcgtaa agtaatttgg
    38101 cactcatcac cgctgagaca ataacaatca tcgggcggat cagcttttgt cctttgctca
    38161 acaccagtcg tgaacccatg cgcgcgccga ggaactgccc caccagcatc acgaatcctg
    38221 tcgcccaaat cactttgcca ccgagaataa acagcagtaa accgccgata tttgacgttg
    38281 cgttgagtaa tttggcgtga gccgtggctt tggcgaggtt gaatccgcac agcgtaacaa
    38341 aggccagcgc gtaaaacgat cccgctgccg gaccaaagaa gccgtcataa aaaccgacgc
    38401 agccaccggc aattaacgcg aagggtaaac cgtacatccg gcgctggcga tcttcttcac
    38461 ccagctttgg catcagcaag aaatagaggc cgatacaaat caccagaatg ggcaaaatct
    38521 gccgtaagac atcagcctga acatactgca ccagcaatgc gccgctcatt gagccgacaa
    38581 aggtcatggc gatattgagt ttctgatcgc ttaaactaac cactttgcgg cgaataaagt
    38641 agatagtggc ggaaatagag ccgccgcagg cttgcagttt attggttgcc agcgcgttag
    38701 cgggagacat ccccgccgcc atcaatgccg gaatagtgag taacccaccg ccaccggcaa
    38761 tcgagtcgat aaatcccgcc agcatggcga caaaaaagag aactcccagc aacagcgggg
    38821 aaaccataaa caggctatta aacgtttcca ttagatcacg tgctcatcca gtagcgcctg
    38881 gcaggaaggc ggcaacggag gcggtgtctt cttctcaggc tttgttgttc ccggttttgg
    38941 aggttcaaac cagctttgca gttctgcccc gcaaccatcg cccggtggcg gtaaaggttg
    39001 atcttcacac tccagactat cggcaggaca acgtaatcgt acatgcatat gcgcgcgatg
    39061 ctggaaccag ggtcgcactt tgcgcaacca gtcgcgatcg gtgcccgcat caaggcaaag
    39121 ttgttgttta atcgccggat taacaaaaat gcgtgtgacg tctttgtcct gggcggcgag
    39181 tttgatcaag ctgaaaattt ccggcttcca cagcgtcggg acaacgtgtt taccgtcgcg
    39241 ggaaactaaa tctaacgctt gcgggcgcaa gagctgcgcg gaggtccagc gtgtttttgg
    39301 cagttgcagg aagatgtcca catccagtcc ggtctggtgg ctggcgtgac cgccgttgaa
    39361 acgaccacca gcaggcatcc ccatatcgcc aatcagcacc gtgcccaagc tcagattgct
    39421 cacctggcta ctcagacgct ggataaacat caccagatcc ggatgtccga aatagcgacg
    39481 ctgatcggta cgcatgacct gataatgttc ggactgtatc ggcagcgtgt cagcgccgac
    39541 gatacaacca ttagaaaaac tgcctatcga ttgtgcgcta cccggcacag gttgggttat
    39601 tttttgccac ggcgtcgctg ccaggctggc gctactggca agcagggcca gcagcgcaat
    39661 cgcggttttg ttcatttttt accagcgtgg aatatcagtc ttcacatcgg cattttgcgc
    39721 ccgttgccgt aacaggtgat ccatcaaaac gatcgccagc atcgcttctg cgatcggcac
    39781 cgcacggatc ccaacacaag gatcgtgacg gcctttggtg atcatctcaa cttcttcgcc
    39841 aaagcggtta atggtacgcc ctggcacggt aatgctggag gttggcttca gcgccatatg
    39901 ggcaatgatt tgctgcccgc tgctgatgcc gccaagaatg ccgcctgcat ggttgctctg
    39961 gaaaccgtct ttagtgattt cgtcgcggtt ctggctgcca cgcagcgcca ccacgtcaaa
    40021 accatcacca atttctacgc ctttcaccgc attgatgctc atcagcgcat gggcgatgtc
    40081 ggcatccagg cggtcaaaga ccggctcgcc aagtccggcg gggacgccac tggcaacaac
    40141 ggtgactttc gcgccgatgg agtcgccctc ttttttcagc gcgcgcatca gttcatctaa
    40201 cgcgtcgatt ttgtccgggt ccgggcagaa aaacggattt tgctcgacct gcgaccagtc
    40261 tttgatttcc agcggaatgt cacccatctg ggtcaggcag ccgcgaatct caatgccaaa
    40321 tttctcggcg agatattttt ttgcaatcgc ccctgccgcc acgcgcatgg cggtttcacg
    40381 agcggaagag cgcccgccgc cgcgataatc gcgcagaccg tatttttgtt cgtaggtgta
    40441 atcggcatgg cctggacgga aaacgtcttt aatggcgctg taatcctgag aacgctggtc
    40501 ggtattttcg atcaacaagc caatgctggt gccggtagta acgccttcaa aaacaccgga
    40561 gagaattttg acctgatccg gctcacgacg ctgggtggtg tagcgcgatg tcccagggcg
    40621 acgacgatca aggtcatgtt gcaggtccgc ttccgtcagc ggaatgcctg gcggaacacc
    40681 atcgacgatg cagccgagcg ccagcccgtg cgattcgccg aaggtggtta cgcgaaagag
    40741 ttgtccaatt gtgtttccag ccatcacggc tccgttattg ttgtgtttgc gtgtttactt
    40801 aatctttata aatcgcgaaa tgttcacggg ccgccagcag ctgctctttg gtgagcataa
    40861 acacaccatc gccgccgtta tcaaactcca gccaggtgaa cggaacatcc ggatattgtt
    40921 ccataagatg taccatgctg ttgcccactt cacaaatcag cacgccatca tcagcgaggt
    40981 aatctgccgc gtttccgaga atgcgacgcg tcagtttcag gccgtcggtg ccagatgcta
    41041 ggcccagttc cggctcgtga cggtattcgt ttggcaggtc ggacatatct tccgcatcga
    41101 catacggcgg gttagtgaca atcaggtcgt actgcacttt cggtaaatca cggaacagat
    41161 cagagcgaat cggaatgacg ttgtggatca gaccgtgttc ttcgatgttc tgttcagcaa
    41221 ccgccagcgc gtcgggagag atatccaccg cgtcaacttc cgcatccggg aaggcataag
    41281 cacaggcaat ggcgatgcag ccgctaccgg tacacatatc taaaatatgc tgcggttgct
    41341 tgctgataag tccggcaaat ttattgttga tcaattcacc aatcggcgag cgcggcacca
    41401 gcacacgttc atcgacgtaa aattcatgac cgcagaacca cgctttgtta gtcaggtaag
    41461 ccaccggaat gcgttcattg acgcggcgga tcacgcgctc aacaatacgg tgtttttcgc
    41521 tggaggtcag acgcgcggtg cgcatatctt ccgggatatc cagcggcagg tagagtgaag
    41581 gcaacaccag ttgcacggct tcatcccacg ggttgtcagt gccgtggccg taccagatat
    41641 tcgccgcgct gaagcggctc accgaccagc gcaacatgtc ctgaatggtt tgcagctcat
    41701 ttactgcttc atcaacgaaa attttatcca cgtattcctc caaggcatga tcgcaataat
    41761 ttcggcggct agtttgccat gaagatgacg ataaatcagc attcacgcgc ggtgagtgag
    41821 gaaaaatacg tttaaaacga tcgattgcgc tacgagtcgg gtaaactgta ggaaaattag
    41881 aaatagagac agataaatga aaaagaaaac aacacttagc gaggaggacc aggctctgtt
    41941 tcgccagttg atggcgggga ctcgcaagat taagcaggac acgattgtcc accgaccgca
    42001 gcgtaagaaa atcagcgaag tgccggtgaa acggttgatt caggagcagg ccgatgccag
    42061 ccattatttt tccgatgaat ttcagccgtt attgaatacc gaaggtccgg tgaaatatgt
    42121 tcgcccggat gtaagccatt ttgaggcgaa gaagttacgc cgtggcgatt attcaccgga
    42181 gctgtttttg gatttgcacg gtttgacgca gctgcaagcc aagcaggagt tagacgcatt
    42241 gattgccgcc tgtcgccgcg aacatgtgtt ttgtgcctgc gtgatgcatg gtcatgggaa
    42301 gcatattttg aagcagcaaa cgccgctgtg gctggcgcaa catccgcatg tgatggcatt
    42361 tcaccaggca ccaaaagagt acggtggtga tgcggcattg ctggtgttga ttgaagtgga
    42421 tgagtggttg ccgccggaat taccgtgaat gctggcatcg aatgcctgat aagacgcttc
    42481 agcgtcgcat caggcatcag tgcacaacga cgagctacta cagcatattc agtacgtact
    42541 ggaatcgaca ggtagaatca ataggttgcc gcgaggtggt atccacgcga agcaccatat
    42601 catgaagctc tttgtcggta atggggtgac gatttggtgt taccgtcact ttggcattgt
    42661 cacagcccga tgaggtgaag gtgagcatct gcgacaccca acgacaataa gcaccacgat
    42721 cgtcagtatc accaaatttg ctgccgtcac acagtttgcc gtctttcatc aggtaggtgg
    42781 agagcgtttt ttcaattatc ccgccggtag actggagcgc taacgtctta tcatgcacaa
    42841 cccgactttc attccaggag acatcataag gccctaacgg tataggtgga caagaagagt
    42901 tactgactgt gcgtccggaa taggttcccg gagcaggatc aataagatca atgctatctg
    42961 catgtgtcga taccagccac gggcagccat gaacgctgga gtcttccttc cagacttcca
    43021 ttcgtatttc ataacaagtc cacatcgtcc tttgcgcacg gctgtcatac tctgcaagcc
    43081 ccggacaaga tgattgtgat atcgctgtca tcgatatgac ttttaatgat ggtgcagggc
    43141 caggtcccgt ccagcttttc aaaggtatcc atccagtgat taaaccggtg ttatttggtg
    43201 tctcataaac accgtgattg acgctatcgg gggtaagaac atcaagataa taagttatct
    43261 tcgtagaata gcttgcatac cctatcggtg tatacattgt tgcaacaatg acattcgcct
    43321 gaactgctgt actgaagaaa agtgatatca gcaggcccaa aatccagcgc attattttca
    43381 tcttaaatat actcaaacgt tgcggttaac tgggcggtaa aattaccagg tgttacatca
    43441 cgcccctctt ttgcctgtaa ccatgctctg aaattaagac tgttattgcc atcattcagc
    43501 acaaaggtca ctcccgtcgt gttattaata gcaacagcag taccatcagt agtttccata
    43561 ccaatgccta cgccctgtgc accagaggta tcatccagag ccagaaatcc cggttgccca
    43621 ctgtcttctg ttccggtcag agtaactttt acgctgtact gcgctggccc acggcaatct
    43681 ttcaggcgaa aaaccaccgg tacacgatcg gactgaccgc gcaccatcaa atctttgtgg
    43741 ctaatattcg caaactgtac ctctgccaac gttccagcct gttccaccgg agtacaggtt
    43801 ttgctcatca ggttgccgta aaaatgcaaa ttattagctg ccagtgctga atgactcgcc
    43861 agcaccagca caccgcccaa cagaaaggtt tgcaccctga tacattgata tttcttcagt
    43921 ttgttcattg ataatccacc tgcaatgtgg cgtaagcgga aaagttaccg tcagtcagcg
    43981 tggcatcgga agcttttaca ggtgcggcct tcaacttcgg caggttggcg ggtgtcacgt
    44041 taagcggggt attcagctta aagggctgac cgttttgctg gagttcaatc cctaatcccg
    44101 tgacatccgt ctgaatcgcc gcatcgttat agttggtctg tgtaccggtc caggttagcg
    44161 tcattaccca ggcgtcatcg gtaatatcag ggtcacacgt tatctggtac ggaacatctt
    44221 tgcggtaatt aaccccgtca atattgtcga taatgacgtc gataaactgg atctcaatat
    44281 catcaccatt gctgacggtg cagaccggag gttcgatcag cgtacccgtg aaggtgatat
    44341 cttccggtgc ggcatgcgtt atggtgcaaa aagcggataa taaaattaac gagagtcgtt
    44401 tcattggtaa tccaccacca ttgtcatgct ggcgttaaac tgtgcgcctg tcagcgtcac
    44461 gccattctgt ttgactggca ctgcttttag ttgtggctgg ctactgacgc taaatggagt
    44521 ccaggtattg tcgccaatcg caaacagaga gttatctgct gcattttcaa tacgaatgcc
    44581 aaacccggta attcccgtgc ttaacaccgt ttcgccattg atcgtggtgg ttgtgccctt
    44641 aaactgcatc tgaaggtcat cgctcaggct attggcacag ttcagggtat aacctgcatc
    44701 tttgcgatat tcagtaccat tgattttttt gatgatcaca tcaccaaaat cgacgttact
    44761 gccttttacg gtacaaggag gtggtgggtc taccagtacg cgcaatgtca atggcacact
    44821 tttcgtttca ccggcaagag cactgcccgt aagcagcact cctcccagcc cggccaaaag
    44881 tagtcgtttc atctcacact cccggcctca gcctttgcgg ctctgctctt catcaacctg
    44941 gcaactgtta ccgttgcagg tgaaaaagag cggcatccga gcgccatagt cgttgacata
    45001 ggtcagtacc ggcacactcc ccatcttcac attcagcggt gcagaggttt tcggcgacat
    45061 cactattggc gagaaaccgg ctgccggatt gccatttttc tgtgcgctgg cattactgaa
    45121 aatgacgtaa tacggcgtcg ggttattgac agtaaattcc tgaccgttgc gggttaaggt
    45181 cactttatgc tgccacgggt tttgcgtggt gtttttttcc agagctttcg gtcgccagaa
    45241 aagtttgata cgcgtctgaa gagcgatttg tagcgtgttg gatttgttcg atttcggcgg
    45301 aatttcgcgc acgttgaaat agaacatcga ttcccgatcg gcaggtaatt tatgaatatc
    45361 cggcaggccc tgaattttaa cctggccatt catcattgct tcaatgcgct gtacaggcgg
    45421 caataccgcc agcggcgagc tgatcttgac gcccttttca tcttcaatcc agctttgcgc
    45481 cagataaggc agtttggggt cgttattacg cagcgtcaca ctgatagatt tatcgctttc
    45541 attaaaaatc aggcgagtac gatcgggcgt tactgacgcc tgggtagaaa aacttaccgt
    45601 tgctgccagt aacgacagta ccgttgtcaa tgttttggca gagagatatt tagtcatgga
    45661 tcattcctta atcacgttat tggttcgatg aaacagacat cggcggttcc attggtgtca
    45721 cctgttgtgt ctgctcctga atgacgggtt gaatatcatg cggtatgacg ggagaacttc
    45781 cccccttttg ctggcatggc aacaacaagc cattgaacag gtcattcggt aacgggtctg
    45841 gcaggctgat atcgcagtga gattcaccct cccagaaaac ggtcatatgt tcacccggtt
    45901 taacgcctgc cagataaaca ttaccttcgt catcaaccaa ccctacttgt tgttgattat
    45961 cgtttttcac ttccgcccca aatggcggat ggctgccatc ctgcaagcgc agcactgcca
    46021 tcgccttctc accgctgatt accgcaaatt tacggtagcc aatagcgcct tccgtcagcg
    46081 ttgcctgaac cactgatttt gtagcttcag cgttttccgg cagtttgttc aaatcgatat
    46141 aagcctggtt gcggtagtaa tcattaacat ctgccaccac cgctttgccg aacatgttgg
    46201 tataaacggc ggaaccatta ccttcaaccg gcacattagc aacgccatca gcgtcgatta
    46261 acagacgtgt accgcccatg ttttgtgtac gatgcagtgc cccgccatga gcggtcagcg
    46321 ttgccccgcc ctgtaaagag atccctgctg aggtgtattg cccttcatgg tagttggcac
    46381 tgagatccac ttttgccaga gtaccatcgt ggctgtagta gccatccacg ctgctgtggt
    46441 tttcgctggt tccggcgctt aacgtgtagt ggctggcgtc atcaatgcgg tgatacagac
    46501 tcacctgatt gctgtcggca ccgctgccat agttgccgtt gtaactgatg gtgctgttgt
    46561 cgccccacgg caaacttaag gacagataaa cacccttgtc agcactttta tcgtattcgt
    46621 agcgataacc ggtcagagaa acgctcaggt tgcgaaggct tcctaaatta aaataatgag
    46681 aaagcatcat gttgtagttg gtctgatcct ggcgatccca gtaggtatgg tgggaatagt
    46741 tgagatagac cgaaacgcgc gcatccgtga agttctggtt ataggtgacc gtatacatct
    46801 ctttgtcgtt accggtgcgc gcccggtcgt cgtcgttcgc atccagatac tcgctcatgg
    46861 tcatgtagtt tttttcagag aagcggtatc cagcaaacgt cacccgactg ttgagttcat
    46921 caaaatcttt ggcatagctg acgcgataag agttgccgtc cagcgttttg ttgccataca
    46981 cgctgttatc gtcgagttga acacgagagt gagtgacatc aaacgctaac gcgcctagca
    47041 gcgccaggtc acgaccgagg cccagcgccg ccgactggta gttttcatcg gctaacgcac
    47101 cgccgtagag cgaccagcca ttggcaatcc cccaggatgc ctcgccgccc gagaagaagc
    47161 tacctgcaac ctgatggtcc cagttttgtg gacgccccat tgtcactttg taacgcacct
    47221 ggccggggcg agtcaggaac ggcatggacg ccgtgctgac gtcgtattcc tgtacctggc
    47281 cgttttgttc ttcaatgcgg acatgcagcg tgccagagac ggaatcgcca atatcctgga
    47341 tgcggaatgg acctgcgggt acctgggttt cgtaaatgac acggcccatt tgggttacgg
    47401 tcactttagc ggtagtgtgt gcaacacctg aaatatcggg ggcgtagccg cgcaggtttg
    47461 gcggtaacat ttgatcatcg gtactgatgc tgccgccaat gtaactgaag ccatcaaaaa
    47521 tatcggagtt gagataatct tcgcccagcg aaagtttggc ttttaatgac ggcaaggcgc
    47581 gccaggcgta gtaacgactc cactcccagt ttttttgcgt accgctatcg tcagtgtcgc
    47641 cgtcatcgtt actgcgggta tgctgataat cgctctgcca gtcagctcgt aagcgccacg
    47701 gcccaacgtt aacgccgacc gtaccattac cgcagatgtc gttagtgtca tcacccccat
    47761 tttcatgccg ggtttgcgcg ttgatactgt aatcggcaat aagcccaggg ataccgtcat
    47821 cccagcgcga aggcggatcc cactcgctgt cggtatattc cagataagcc tgcggtacag
    47881 aaacaaggag tgcagactgg cctaaatcgc cgctaatttc tgtacctgct aaaagtgccg
    47941 tattcagaca ctgaccatcg cggatccatt gcaaattctt tgcaatatct tccttcagac
    48001 caaactgggc aaccagctct ggcgataagc aggcatatga tttattcgga tcgttttcag
    48061 ttgcgtacca gtagatatcg tagtcgtcag gtaacggctg attatttaca tgaacgcgta
    48121 agttatattt tcccggttct acatagcctt tttgcgaaaa acggcccaaa tcaattttgg
    48181 tattgccttt taaatcaaga aatcggccat caaactgaat ggtatcgtct gccagggcat
    48241 taaatggact tccagaaatt gccagagcaa tccaccacga aagcccccgt aaacggagaa
    48301 aagaatgttc tggcatcggc gtatccatcc atgatttttt ataatttagt tatttggtag
    48361 ttataaaccc cataactttg cagctatggg ggaaatacat tagagatacg tgatctggaa
    48421 cgtcgtcagc gtttcaaacg gtgccggtgt tggtgttaca gcatccgttt cacccactaa
    48481 ccatgcttta aagttcagag tttgtttagg cttgcctttt cccgttgaac tgtccttcac
    48541 gataggttgc ttaatctctg ctcccagtgc ataacctgta ccgtgctggt caccgataac
    48601 caggctaacg tttggaattt cagttccatt atccatgtta gacagagaat agttatctgc
    48661 gttagtggag tacggattac cggtgaatgt ggtagccatt gtcgtttctg tattgaaatt
    48721 acagtcctgt aaacgaatct ggaaatcttt tggtgtggtt gttttgccgc cggttaaaga
    48781 acccgtaccg atgctgccca tatccacttc cagtttatga tcgtcaggaa caattgagca
    48841 cggggagtct tcaattgtgc cgtaaaatct cactgtaccg ttgttccctg cggcaacgac
    48901 tgatgctgaa gtcagcgccc ccatgagcat tgccgtggca actgctgctt ttacaaactt
    48961 actcattatt tgtccttaaa tcataaataa ttagaatata agccaactga aatattgacg
    49021 tagagcttct tcaattttta cagcgacttt taagcagcca atgaacagat tttcagctcc
    49081 tgaaaaaaca ggttccttga tataatattc tttggcgtgc cgaacatgtt gatattttaa
    49141 tatgactgat gcaatttaaa ttttctaaca ttcaaagtta gaaatactta attaatggac
    49201 ggggataata attactgaaa tttttcatct cagttatgaa gaatgagttc aaaaaaacgg
    49261 caaatgcaaa aactcagtga ataaatcaaa gtcaaaaaca atcacatggc aatgactatc
    49321 ttgtgatatt aatttcgcaa cgatataata ttaaaacact ataattaata taaaatcaag
    49381 acccaataa
//
LOCUS       DAPOZO010000028        49297 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPOZO010000028 DAPOZO010000000
VERSION     DAPOZO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.028"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(8..457)
                     /gene="rplI"
                     /locus_tag="ROI21_001757"
     CDS             complement(8..457)
                     /gene="rplI"
                     /locus_tag="ROI21_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDX5110685.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(499..726)
                     /gene="rpsR"
                     /locus_tag="ROI21_001758"
     CDS             complement(499..726)
                     /gene="rpsR"
                     /locus_tag="ROI21_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDX5110686.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(731..1045)
                     /gene="priB"
                     /locus_tag="ROI21_001759"
     CDS             complement(731..1045)
                     /gene="priB"
                     /locus_tag="ROI21_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDX5110687.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1052..1447)
                     /gene="rpsF"
                     /locus_tag="ROI21_001760"
     CDS             complement(1052..1447)
                     /gene="rpsF"
                     /locus_tag="ROI21_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDX5110688.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            1774..2049
                     /locus_tag="ROI21_001761"
     CDS             1774..2049
                     /locus_tag="ROI21_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX5110689.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHPILIREPGSNGNMHASADIYR"
     gene            complement(2178..2864)
                     /gene="ulaF"
                     /locus_tag="ROI21_001762"
     CDS             complement(2178..2864)
                     /gene="ulaF"
                     /locus_tag="ROI21_001762"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDX5110690.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(2864..3718)
                     /gene="ulaE"
                     /locus_tag="ROI21_001763"
     CDS             complement(2864..3718)
                     /gene="ulaE"
                     /locus_tag="ROI21_001763"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDX5110691.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(3728..4378)
                     /gene="ulaD"
                     /locus_tag="ROI21_001764"
     CDS             complement(3728..4378)
                     /gene="ulaD"
                     /locus_tag="ROI21_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDX5110692.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(4392..4856)
                     /gene="ulaC"
                     /locus_tag="ROI21_001765"
     CDS             complement(4392..4856)
                     /gene="ulaC"
                     /locus_tag="ROI21_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDX5110693.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(4866..5171)
                     /gene="ulaB"
                     /locus_tag="ROI21_001766"
     CDS             complement(4866..5171)
                     /gene="ulaB"
                     /locus_tag="ROI21_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDX5110694.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(5187..6584)
                     /gene="ulaA"
                     /locus_tag="ROI21_001767"
     CDS             complement(5187..6584)
                     /gene="ulaA"
                     /locus_tag="ROI21_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDX5110695.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            6939..8003
                     /gene="ulaG"
                     /locus_tag="ROI21_001768"
     CDS             6939..8003
                     /gene="ulaG"
                     /locus_tag="ROI21_001768"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDX5110696.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8111..8866
                     /gene="ulaR"
                     /locus_tag="ROI21_001769"
     CDS             8111..8866
                     /gene="ulaR"
                     /locus_tag="ROI21_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDX5110697.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8863..9612)
                     /gene="yjfP"
                     /locus_tag="ROI21_001770"
     CDS             complement(8863..9612)
                     /gene="yjfP"
                     /locus_tag="ROI21_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDX5110698.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLADRLAVGGASMGSMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLADRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            9794..10123
                     /gene="bsmA"
                     /locus_tag="ROI21_001771"
     CDS             9794..10123
                     /gene="bsmA"
                     /locus_tag="ROI21_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDX5110699.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            10272..10547
                     /gene="yjfN"
                     /locus_tag="ROI21_001772"
     CDS             10272..10547
                     /gene="yjfN"
                     /locus_tag="ROI21_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDX5110700.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(10664..12289)
                     /locus_tag="ROI21_001773"
     CDS             complement(10664..12289)
                     /locus_tag="ROI21_001773"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDX5110701.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRHLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(12373..13536)
                     /locus_tag="ROI21_001774"
     CDS             complement(12373..13536)
                     /locus_tag="ROI21_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDX5110702.1"
                     /translation="MLRHNVPVRWDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(13539..14177)
                     /locus_tag="ROI21_001775"
     CDS             complement(13539..14177)
                     /locus_tag="ROI21_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDX5110703.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSRARGHWGG"
     gene            complement(14187..14585)
                     /locus_tag="ROI21_001776"
     CDS             complement(14187..14585)
                     /locus_tag="ROI21_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDX5110704.1"
                     /translation="MHILDSILAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(14603..15262)
                     /locus_tag="ROI21_001777"
     CDS             complement(14603..15262)
                     /locus_tag="ROI21_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDX5110705.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGENIDDIDDIKLFVYE
                     ESYGISKESHWRETINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(15313..16011)
                     /locus_tag="ROI21_001778"
     CDS             complement(15313..16011)
                     /locus_tag="ROI21_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDX5110706.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALKALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(16030..16431)
                     /locus_tag="ROI21_001779"
     CDS             complement(16030..16431)
                     /locus_tag="ROI21_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDX5110707.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(16557..17288)
                     /gene="rlmB"
                     /locus_tag="ROI21_001780"
     CDS             complement(16557..17288)
                     /gene="rlmB"
                     /locus_tag="ROI21_001780"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDX5110708.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLNQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(17379..19820)
                     /gene="rnr"
                     /locus_tag="ROI21_001781"
     CDS             complement(17379..19820)
                     /gene="rnr"
                     /locus_tag="ROI21_001781"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDX5110709.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(19859..20284)
                     /gene="nsrR"
                     /locus_tag="ROI21_001782"
     CDS             complement(19859..20284)
                     /gene="nsrR"
                     /locus_tag="ROI21_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDX5110710.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(20489..21787)
                     /gene="purA"
                     /locus_tag="ROI21_001783"
     CDS             complement(20489..21787)
                     /gene="purA"
                     /locus_tag="ROI21_001783"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDX5110711.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(21891..22088)
                     /locus_tag="ROI21_001784"
     CDS             complement(21891..22088)
                     /locus_tag="ROI21_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDX5110712.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(22170..23174)
                     /gene="hflC"
                     /locus_tag="ROI21_001785"
     CDS             complement(22170..23174)
                     /gene="hflC"
                     /locus_tag="ROI21_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDX5110713.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(23177..24436)
                     /gene="hflK"
                     /locus_tag="ROI21_001786"
     CDS             complement(23177..24436)
                     /gene="hflK"
                     /locus_tag="ROI21_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDX5110714.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSTTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(24522..25802)
                     /gene="hflX"
                     /locus_tag="ROI21_001787"
     CDS             complement(24522..25802)
                     /gene="hflX"
                     /locus_tag="ROI21_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDX5110715.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(25878..26186)
                     /gene="hfq"
                     /locus_tag="ROI21_001788"
     CDS             complement(25878..26186)
                     /gene="hfq"
                     /locus_tag="ROI21_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDX5110716.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(26272..27222)
                     /gene="miaA"
                     /locus_tag="ROI21_001789"
     CDS             complement(26272..27222)
                     /gene="miaA"
                     /locus_tag="ROI21_001789"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDX5110717.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(27215..29062)
                     /gene="mutL"
                     /locus_tag="ROI21_001790"
     CDS             complement(27215..29062)
                     /gene="mutL"
                     /locus_tag="ROI21_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDX5110718.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRIAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(29072..30409)
                     /gene="amiB"
                     /locus_tag="ROI21_001791"
     CDS             complement(29072..30409)
                     /gene="amiB"
                     /locus_tag="ROI21_001791"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDX5110719.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGAAAQTASTVTTPDRTLPN"
     gene            complement(30428..30889)
                     /gene="tsaE"
                     /locus_tag="ROI21_001792"
     CDS             complement(30428..30889)
                     /gene="tsaE"
                     /locus_tag="ROI21_001792"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDX5110720.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(30861..32408)
                     /gene="nnr"
                     /locus_tag="ROI21_001793"
     CDS             complement(30861..32408)
                     /gene="nnr"
                     /locus_tag="ROI21_001793"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDX5110721.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRTAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLGWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            32407..33546
                     /gene="queG"
                     /locus_tag="ROI21_001794"
     CDS             32407..33546
                     /gene="queG"
                     /locus_tag="ROI21_001794"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDX5110722.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(33529..33582)
                     /locus_tag="ROI21_001795"
     CDS             complement(33529..33582)
                     /locus_tag="ROI21_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110723.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(33816..33891)
                     /locus_tag="ROI21_001796"
     tRNA            complement(33816..33891)
                     /locus_tag="ROI21_001796"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33856..33858),aa:Gly,seq:gcc)
     gene            complement(33927..34002)
                     /locus_tag="ROI21_001797"
     tRNA            complement(33927..34002)
                     /locus_tag="ROI21_001797"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33967..33969),aa:Gly,seq:gcc)
     gene            complement(34160..34235)
                     /locus_tag="ROI21_001798"
     tRNA            complement(34160..34235)
                     /locus_tag="ROI21_001798"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34200..34202),aa:Gly,seq:gcc)
     gene            complement(34446..34991)
                     /gene="orn"
                     /locus_tag="ROI21_001799"
     CDS             complement(34446..34991)
                     /gene="orn"
                     /locus_tag="ROI21_001799"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDX5110724.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            35086..36138
                     /gene="rsgA"
                     /locus_tag="ROI21_001800"
     CDS             35086..36138
                     /gene="rsgA"
                     /locus_tag="ROI21_001800"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDX5110725.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            36235..37203
                     /gene="psd"
                     /locus_tag="ROI21_001801"
     CDS             36235..37203
                     /gene="psd"
                     /locus_tag="ROI21_001801"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDX5110726.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            37225..40548
                     /gene="mscM"
                     /locus_tag="ROI21_001802"
     CDS             37225..40548
                     /gene="mscM"
                     /locus_tag="ROI21_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDX5110727.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(40699..42201)
                     /gene="yjeM"
                     /locus_tag="ROI21_001803"
     CDS             complement(40699..42201)
                     /gene="yjeM"
                     /locus_tag="ROI21_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDX5110728.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(42420..43397)
                     /gene="epmA"
                     /locus_tag="ROI21_001804"
     CDS             complement(42420..43397)
                     /gene="epmA"
                     /locus_tag="ROI21_001804"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDX5110729.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            43722..45530
                     /gene="frdA"
                     /locus_tag="ROI21_001805"
     CDS             43722..45530
                     /gene="frdA"
                     /locus_tag="ROI21_001805"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDX5110730.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            45523..46257
                     /gene="frdB"
                     /locus_tag="ROI21_001806"
     CDS             45523..46257
                     /gene="frdB"
                     /locus_tag="ROI21_001806"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDX5110731.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            46268..46663
                     /gene="frdC"
                     /locus_tag="ROI21_001807"
     CDS             46268..46663
                     /gene="frdC"
                     /locus_tag="ROI21_001807"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDX5110732.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPESWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            46674..47033
                     /gene="frdD"
                     /locus_tag="ROI21_001808"
     CDS             46674..47033
                     /gene="frdD"
                     /locus_tag="ROI21_001808"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDX5110733.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            47096..48229
                     /locus_tag="ROI21_001809"
     CDS             47096..48229
                     /locus_tag="ROI21_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDX5110734.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLI
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFHPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            48317..48850
                     /gene="blc"
                     /locus_tag="ROI21_001810"
     CDS             48317..48850
                     /gene="blc"
                     /locus_tag="ROI21_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDX5110735.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     QEGFDVSKFIWVQQPGS"
     gene            complement(48847..49164)
                     /gene="sugE"
                     /locus_tag="ROI21_001811"
     CDS             complement(48847..49164)
                     /gene="sugE"
                     /locus_tag="ROI21_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDX5110736.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT        11919 a        13481 c        12307 g        11590 t
ORIGIN      
        1 taacgaatta ttcagctact acgtttacga tcactttcgc gaatacttcg ctgtgaacct
       61 ggaagctcac ttcgtgttcg ccagtggtac gcagaacgcc gttcggcaga cgaacttcgc
      121 tcttagccac ttcaacgcca gctgcagtta cagcgtcagc gatgtcgcga gtaccgatgg
      181 aaccgaacag tttaccttcg tcgccagctt tagacgcgat ggtaacagtt tccagtgcat
      241 tgattttctc agcgcgagca ttagcagctg ccagaacttc agccagttta gcttccagtt
      301 cagcgcgacg tgcttcgaag aattcaatgt ttttcttggt agctggaaca gctttaccct
      361 gcggtaccag gaagttacga gcatagcccg ctttaacgtt tacctgatca cccaggctac
      421 ccaggtttgc tactttatca agcagaataa cttgcattac cttatcctct caaagtcgta
      481 ttaatggacc gtgaccgatt actgatggcg atcagtgtac ggcagcaggg acaggtagcg
      541 agcgcgtttg atagcgcgag ccagctgacg ctggtatttt gcacgggtac cggtgatacg
      601 gcttgggaca atcttaccgc tttcggtgat gtagtttttc agcgtagcga tatctttata
      661 gtcgatctct tgaacgcctt ccgcggtgaa acggcagaac ttgcgacgac ggaaataacg
      721 tgccatatgg ctagtctcca gaatctatca attcaatctg ctcggcatgc aaaaccattt
      781 tgctcagtcc gttctttgcc ttgtggcatg aaatgaaccc ctgaacggtt atgcgactgc
      841 cgaccgttat actgtgagta atggcctggt tttcgtgtcc gctaacaata acgggcattt
      901 ggcaccacgc ctgccggtga aaaccggctt cctcctgcac agaacgatgc tcaagcacga
      961 actggcagtg aggaattcct gatggactga cctttcgaag gggagtcctg cacacggtgc
     1021 cggacaacac cagacggttg gtcatcagaa attactcttc agaatcccca gcttcagcat
     1081 catcagcggt ttcgtttgcg aaatcatcgc gacgctcacg gcgctcgtct ttcgctttaa
     1141 ccatcggaga tgcttcggta acagcgtgct tggtacgcat aaccatgctg cggataacgg
     1201 catcgttgaa gcggaaggta gtttccagct catcgatcac ttcctgcgga gcttcaacgt
     1261 tcatcagaac gtagtgtgct ttgtgcagtt tgttgatcgg gtaagccagc tgacggcggc
     1321 cccagtcttc cagacggtgg atcttgcctt ctgcaccagt gatggcagca gtgtagcgct
     1381 cgatcatgcc cggaacctgt tcgctctggt caggatggac cataaaaacg atttcgtaat
     1441 gacgcatcga attgctcctt acggattatt cagcctcctg tctgggtcag ccgaatcccg
     1501 gggaggcaag gaacgtgtta aaggtcggct gaaaaatgac gcgttatagt actgacatac
     1561 cccgctaaac tcaaggccag ccgacaaata aatctcatct ttcaccaaat cgtactaaca
     1621 cgctgatttg aagttcatca cacttcatca tggtgttttt ttgaacaact gcatcagaaa
     1681 tggtcaccat caacttcttt atcattgatt aaaattaaat cagaagatca tcacagagga
     1741 gagcacacaa agtcagccgt tgaggaggta tctatgttca gtcgtgtttt agcccttctg
     1801 gctgtgcttt tgctaagtgc aaatacatgg gcagccattg aaattaataa ccaccaggcc
     1861 agaaatatgg acgatgtgca aagcttaggc gtgatttata tcaaccataa cttcgccact
     1921 gaaagtgaag cacgtcaggc attaaatgaa gagacagatg cgcagggcgc aacgtactac
     1981 catccgatcc tgatacggga accgggaagt aacggcaata tgcacgccag cgcggatatt
     2041 tatcgctagc accaggtata accaacgaaa cattgccata gtttgctttg cccccttcgc
     2101 agggggcttt ttttgaaaaa cggctgcgga attagaccag ttatctcccg aagaaggaaa
     2161 tttccgcagc gcgtgtttta cttctgcccg taataagcgt tgggaccgtg cttacgcatg
     2221 aagtgtttat tcatgaggta gctgtcgatg tgattcaatt gtgggttaat gccgcgggca
     2281 atccacgcca tttttgccac ttcttccatc accaccgcgt tatgcaccgc atcgtgagca
     2341 tctttccccc aggcgaacgg cccgtgctgg tacaccacaa tccccggtgt atgcagaggc
     2401 tcggcgttgc ccagcgtttc gataatcact ttgccggtgt tcagttcgta ctcaccctgc
     2461 acctcttctt cgcttaatcc gcgcgtacac gggatatcac caaagaagta gtcggcgtgc
     2521 gtagtgccta acgccgggat cgccagcccc gcctgcgccc atgcggtagc atgagtggag
     2581 tgggtatgga caatgccgcc aagcgacgga taacgacggt agagttcgag atgcgtcgcg
     2641 gtgtcggaag acgggcgata ttccccttcc accaccttgc cgctcatatc aaccaccacc
     2701 atatcggccg ctttcatggt ttcgtaggca acgccgctgg gcttgatcac caccagcccg
     2761 cgttcgcggt caatggcgct gacgttgccc caggtaaagg tcaccagccc gtagcgcggc
     2821 agatccatgt tggcttcaaa tacctgctgt tttagctttt gcattatgcc gcctccacca
     2881 tgcccgcttt cgccatgcgc gctttcaccc aatcacgcgc tttcgccact tctgccgccg
     2941 ggtcttccgc cgtttcgctc cacatctcaa tcaggtacgg cccgcaatag ccactctgtt
     3001 tgagcgtttc gaagcaacgt tcgaaatcca ccacaccttc gccaaacggc acgtttttga
     3061 agacgcctgg tttggtgtct ttcacatgca ccgcgacgat atgcccaatt ccggcctgca
     3121 attccatctg cacgtcgttg tcccacgccg acaggttgcc gatatccggg tagagctgga
     3181 accacgggtt gttgagatag tgcgcgtaac ccaacgcctt actgatggag ttcatcaacg
     3241 ggtaatccat gatctccatc gccagcgtca cctgcgcgcg gcttgccatc tcaacgctct
     3301 ctttcaggcc gtcacggaaa cgacgacgcg tttcgttatt ggcttcctga tagtaaacgt
     3361 catagcccgc cagttggatt acgcgaatgc caacgtcctg ggcgaactgg atagctttac
     3421 gcataatctc cagcccctgc gcccgtaccg cgtcatcttc gctgcccagc gggaaacgac
     3481 gatgagcaga aaggcacatg gacggcacgc gcacgccggt ttcaacaatc gcattcacca
     3541 gcgccagacg ctgctcgcgg ctccagtcga ggcgcgacag gcgatcgtct gtttcatcta
     3601 ccgacatctc gacaaaatcg aagcctaacg tttttgccag ttgcagccgt tccagccagc
     3661 actccccggc ggggagcgct ttttcataga tgccaagcgg gatttgtttg gacaacatat
     3721 ccgctcctta gccccacagt tcggcgatgg aacgtttgaa ctgacgcgcg gcttcaaccg
     3781 gagaagcggc atcacggata ctacggcccg cgataaagac gtgaatcgga atgcctttga
     3841 acagcggcag atcttccagc gccaggccac cggtgacggt gactttgaag cccatatcgg
     3901 aaagacgttt gatcgcggtg atatctgctt cgccccatgc cacgcctgcg gcctgcgcgt
     3961 cacggctgcg gtgataaacc acctgctgaa tgcctgcgtc acgccactgt tgcgcctgtt
     4021 cccaggtcca gtaaccggtc agttcgatct gcacgtcgcc gttaaactct tttgctacgt
     4081 ccagcgcacc tttggcggtg ttgatatccg cacagcaaat cacggtcacc cagtcagcgt
     4141 tggcttcgaa gcacatacga gaaagaattt tgcccgcatc ggcaattttg gcgtctgcca
     4201 gcacgatttt gtgcgggtag agtgctttga ggtcacgaac cgcacgcacg ccttcgccca
     4261 cacacagaat ggtgcccact tcgataatgt cgacttcttc ggcaatcagg cgagtggttt
     4321 cgtaagcgct atccatagtc tggttgtcca gcgcgacttg caacatcggt aatgacatgt
     4381 tcaattcctt cttaagctgc cgcgttggtg cggtcaatta aatccagtac ttcctgctcg
     4441 gtacggcagg cacgtaaacg gtcgaaattc tcttcatctt caaacaggtt gacgatctgc
     4501 atgatgccca cttcctggtg ggtgttggca tcaaccgccg ccatggtgat gaggatatcc
     4561 accggatcgt tatcttcgtg gttgaactcc agcggctttt tcagcgtcac cagcgcaaaa
     4621 ccggttttct taacgccctc ttccgggcga ccgtgcggca ttgccaggcc tggtgcgagc
     4681 acaaagtacg gaccgtgctg ttcaaccgca tccagaatcg cctggtagta acgcggctcg
     4741 acaacatccg ccgccaccag caggtcaacg ccgattttta ctgcgtcctg ccatgtctca
     4801 gcttcagcct gcaggcggat ggatttattc tccgccagcg aatcacgtaa tttcatggcg
     4861 cgtccttact tcacatcctg cgggaaatgc tctttgatca cttccagcag tttcgggcca
     4921 aagtcagcag gagagagcat gttgcgcacg ccaaccacat atttgttgcc ggtcacggtg
     4981 atttcgcccg caatgtgcgt agaagcgatg atgatatccg cgccgctcaa ctcgcttttg
     5041 tattcgccaa ccgcgcagct gtttaccgtg tggtcaatgt ttgattgggt taaaaactgg
     5101 tccactttca ttttcatgat catggaactg ccttgtccgt tgccacacac agccagaata
     5161 cgtacggtca taatcaaaac tccttattaa gcagactgtt ctgccagttg tttttctgca
     5221 tcttcttctg cgcgcagcgc gcggccagcg aagaacatat aagccagtgc aattacaatg
     5281 atgacggcca taaaggcgat accgatggag aagaagccct gcatcatcgg tggtgccaga
     5341 atcgaccagt ccgccatgcc catccaggca ctcataccgg tgagtttcac cgcccagacg
     5401 caaccaaaga tttcgatcat ccccatcacc agacaaatct tcagtgccgc acgccagccg
     5461 ccgaagtggt tagcgaacac gccgatggtg gcgttggaga agaacatcgg gataaagccg
     5521 ggaataatca ggatcgagga accgcaggcg accaggatgc caactgcaat cagctgaccg
     5581 atggtgcccc acataaagcc ccagaccacg gcgttcggcg cgaagctata gatagccgca
     5641 cagtcaatcg ccaggaccgc acctgggatc aggcgctggg agataccgtt aaacgcttca
     5701 gagagttccg ccacaaacat gcgcacgccc tgagtgatga tgaagatcgc caccgcaaag
     5761 gagaaaccgg tttgcaggat gtacaccgtc cagttcactt tgcctgccat cgcctgcacg
     5821 gtgtcgatac cgaaggagag cagaatggca ccaaagaaga tggtcatgac aatcgccgtg
     5881 gagacaatgt tgtcgtggaa aatattcagc cagcctggca gtttgaggtc ttcaacgctc
     5941 tcctcttttt tgcccaggaa cggcgcgact ttataagcaa tccatgatgc aaactgttgc
     6001 tggtgaccga tggagaaacc acagccgtcc gtcacttcct gagtcggctt gtacatcatg
     6061 ttggaggtaa tgccccagta gagcgaaacc agaatcgccg tgcagataat ggtcgtccac
     6121 atggagtagc cgaaaataaa caacgtaacg gcaatcagcc ccgcctgctg gaacatgata
     6181 tgaccagtca gcatgatggt acgaataccg gtaatgcgac gcagcagcac gtaacaaatg
     6241 ttcagcgcca gtgccagcag caccgcgtac cctacccagc tataggcatc gcccatgcgg
     6301 tcgatggttg ccatcatcga cgcgtaagta tcggaaattg cgccgttaat gccgtagact
     6361 tcggacattt tcgccaccac cggtttgaag gtgctggtga ggatgccgga ccctgcctgc
     6421 aacaacatga aaccaattat ggttttaatc gtgcctttaa taataacgct gacactttta
     6481 cgcagtagga tgtagcccag acaggtcaca atacccagca acaacggggc attggtcata
     6541 acctggttaa aaaacacggt aaagatgttg tagaggatct ccataacgat ctcctgaaga
     6601 agaggtaacc gggtattcca cacacccgga atgttgtgca ctcactctaa ttttcaaaag
     6661 taatcacaac aagattattt atgattaaat gtgacgcgac ccgcaaataa ttccacatgg
     6721 atagagtggg attcacacaa cgaaataatt attcatttaa attcatttag ttaattgaat
     6781 tacacattcc attaaatctt tccacactca aaatcaacaa caacttccct ggcaaaaagt
     6841 caaaaaatgg tcattgcctt cctgaaaaaa tagtgtcagg attaatcaaa accaatcacg
     6901 tgttgattcg aattgaacta tcatgaaggt aaatggcgat gagtaaagtg aaaagtatca
     6961 cccgagaatc ctggatcctg agcactttcc cggagtgggg tagctggctg aatgaagaaa
     7021 ttgaacaaga gcaggtcgcc cctggcacat ttgcgatgtg gtggctaggc tgcaccggga
     7081 tctggctgaa atcagaaggt ggcaccaacg tttgcgttga tttctggtgc ggcactggca
     7141 aacaaagtca cggtaacccg ttaatgaaac agggtcacca gatgcagcgc atggctggcg
     7201 tgaaaaaact gcagccaaac ctgcgcacca ccccgtttgt tcttgatccg tttgcgattc
     7261 gccagatcga cgcggtactg gcgactcacg atcacaacga tcatatcgac gttaacgtcg
     7321 ctgctgccgt gatgcagaac tgtgctgatg acgtaccgtt cattggaccg aaaacctgcg
     7381 ttgatttgtg gattggctgg ggcgtaccga aagagcgttg catcgtggtc aaaccgggcg
     7441 atgttgtaaa agtgaaagac attgaaattc atgcgcttga cgctttcgac cgtactgcac
     7501 tgatcaccct acctgccgat caaaaagcgg ctggcgtact gccagatggc atggacgatc
     7561 gcgcagtaaa ctacctgttc aaaacccctg gcggttccct gtatcacagt ggcgattccc
     7621 actactctaa ctattatgcg aagcacggta acgaacatca gatcgatgtg gcgttaggat
     7681 cgtatggtga gaacccgcgc ggtattaccg acaaaatgac cagcgccgat atgctgcgta
     7741 tgggtgaagc cctgaatgcg aaagtggtta tcccgttcca ccacgacatc tggtcaaact
     7801 tccaggccga tccgcaagaa atccgcgtgc tgtgggagat gaaaaaagat cgtctgaagt
     7861 atggcttcaa gccgtttatc tggcaggtgg gcggcaaatt tacctggccg ttggataaag
     7921 acaacttcga gtaccactat ccgcgtggtt tcgatgattg cttcactatt gaaccggatc
     7981 tgccgttcaa gtcattcctg taatctgtcg tttataccgg atagcgtgga gtagttcgcg
     8041 ctatccggcg tagtatttcc tggctatttc aaatatcatc taaaaaaatc aaattttatc
     8101 ggaatagctc atgactgaag cacaaagaca tcaaatcctc ctggaaatgc tcgcacaatt
     8161 gggctttgtg accgttgaga aagtcgttga gcgtctggga atttcgcctg ccactgcgcg
     8221 acgcgatatc aataaacttg acgaaagcgg caaactgaaa aaagtgcgca atggcgcaga
     8281 agctattacc cagcagcgcc cacgctggac gccgatgaat ctgcatcagg cgcagaatca
     8341 cgatgaaaaa gtacgtattg ctaaagcggc ctcgcagctg gttaatccgg gcgaaagcgt
     8401 agtcatcaac tgcggctcca ctgcgtttct gcttgggcgg gaaatgtgtg gcaagcctgt
     8461 gcaaatcatc actaattatc taccgctggc aaattacctg atcgatcagg agcatgacag
     8521 cgtgatcatt atgggcggac agtacaacaa aagtcagtcc atcactttaa gcccgcaagg
     8581 cagcgaaaac agtctctatg ccgggcactg gatgtttacc agcggcaaag ggctgacagc
     8641 agaagggttg tataaaaccg atatgttgac ggcaatggca gagcagaaga tgctgagcgt
     8701 ggtagggaaa ctggtggtac tggttgatag cagtaagatt ggcgaacgcg cgggaatgct
     8761 ttttagccgt gccgatcaaa tcgatatgct gatcaccggc aaaaatgcta acccggaaat
     8821 cctgcaacaa ctggaagcgc aaggggtcag cattctgcgt gtttaaagat gctggcggaa
     8881 aaatgtcacg gcagcatcta acgcctcagg cgtaatgcgg tgacgcacgc ctggctgcca
     8941 tgaacaggtt agcagtttat cccgtcccgt ctcgcttaag gcctgctgca aacgtagtga
     9001 ttcgtcggca ggcacgacgt catcgtcaag gccatgccac agcagtagag gtctgtcagc
     9061 aagttgttcc aggtggtttg tcgcttccca ctctgccagc ggcgcgacaa tgttattgaa
     9121 ttcattctgc tgtgctgccg tttcaggtat cagcggtgga aacagtgaac gggcgagcga
     9181 tgtaaaatag cccgatccca tcatgctggc ggtacatctc accgtggggt ggcgagcggt
     9241 aattcccaat gccgtcatcg agcccatcga cgcgccaccg actgccagac ggtcagcaag
     9301 cagccagttt tcttcggcta ttgccgcacg taacgtagtg aattcctgca tactttgtag
     9361 caagatttgc cagaattgat ttaaccgccg cgctgcgtca ccactaaaac ggctaccatg
     9421 atcgggcgca tccggcatga tcacccgcaa accagcctgc gccagcgcaa cggcaaaata
     9481 gctatacacc agactggacg aagtaaagcc gtgataaaaa atcacgcacg gtaatggggt
     9541 atctttttgc ccgacaggat aagcatgaag aacgggaata tctgccagct cgcgtgattc
     9601 tatttcaatc atctgtctct cctttttgtc tggtatgaca tgaagaaatc cttagcacaa
     9661 gtttctgctg gcggtattag ttcataatgt tgagatgtgg gttacgcttt catcacgttt
     9721 tcattcgaaa atcgcgtacc aggtaacaat tcgggaacat tcccccaaag ttaaattaat
     9781 aaggcactac actatggtta gcaggaaacg taatagcgtt atttaccggt ttgccagttt
     9841 attattggtg ttgatgttaa gtgcctgtag cgcactgcaa ggtacgccac agccagcacc
     9901 gccagtaacg gaccatccgc aagagattcg ccgcgaccag acgcaagggt tacagcgaat
     9961 aggtagcgta agcacgatgg ttcggggttc tccggatgac gcattagcag aaatcagagc
    10021 gaaagctgtc gctgcaaaag ctgattatta cgttgtcgtc atggtggacg aaaccatcgt
    10081 gacaggacag tggtattcac aagccatttt gtaccgtaaa taaatcagac aacttacata
    10141 gtcagcttta cattgctttg cgtccctatg cgtttacctg tgcacaataa attaccggct
    10201 ggaagctcgg gcgtttatta ccgcgatgga atgccctgca acatgtgggg aaacgaaaaa
    10261 tggagctgac gatgaaacaa ttacttgcct caccctcgct gcaattagtg acttatcctg
    10321 cgagcgccac ggcacagtct gcagaattcg ccagtgctga ctgcgtgacg ggcctgaatg
    10381 aaataggtca gatctcagtt agcaatattt caggggatcc gcaggacgtg gaacgtatcg
    10441 tggcgttaaa ggccgatgaa caaggtgcgt catggtaccg cattattacc atgtacgaag
    10501 accagcagcc tgataactgg cgcgtacagg cgatactgta tgcatgagta tcatctacag
    10561 caaatgaaca atgccggatg cggcgcgagc gccttgtccg gcctacaatt ttcacatatt
    10621 tcattgagtt aattgaaacc agcgtcgcat cagtcgtata cgcttacaca cacactcccc
    10681 ccgtcgcccg cagcaataaa tcattctgga tctgctctga caaccgtacg ccgccgcgcg
    10741 tatctaacat cacctgacac cacgcctgcg ccattggcgg agaggcatat ttcaacattt
    10801 gcgcaccgca gcccagcagg aatagctgat gagtaatctc tcgccccagt tcttcagcag
    10861 gcttacgcag ctgctgctgt aaacgacgaa ccgcgcgatc aaaatagcga tcctgccctt
    10921 tcacttccac aaatgcttcc gacaataagt cgtatacacc cgcttgctta ttgagaacgc
    10981 gcagcacatc caggcacata atattaccgg aaccttccca aatactgttt accggcatct
    11041 cccggtaaag ccgcggcagc tcgctctcct cgcaataacc aatgccgccc agcacctcca
    11101 tcgcttcggc cacaaacggc atacctcgtt tgcagatcac aaatttcgcc gcaggcgtaa
    11161 ataaacgcgc ccacagggct tctttggcat cggcacgccg gtcccacgct cgcgcaagac
    11221 gaaacagcaa cgccgtttgc ccttcaagct gaagtgccat gcgacttaag acatgacgca
    11281 taaggggctg ttggatcaat ggattaccaa aaacatggcg ttgatgtgca tgataaatcg
    11341 ccagcgaaaa tgcacggcgc atcatggcat ggctacccag ggcgcaatca aaacgcgtca
    11401 tcccccccat tttcaggatc agacgaattc cttccccttc ctgccccaac aaccagccaa
    11461 tggcatcctg aaactccact tcgcagctgg cgttagagcg attacccagc ttatctttca
    11521 gccgctcgag gcgaatcgcg ttgcgttgcc cgtcaggcaa aaagcgcggc acaaaaaagc
    11581 aggacaaacc gcccgtagtc tgcgccagca ccagatgcgc atcgctttgc ggcaccgaga
    11641 aaaaccattt atgccccacc agccgataag agccatcttc cagacgctct gcgcgggtgg
    11701 tgttgctcat gacatcggaa ccgccctgct tttccgtcat tcccatgcca atcaacaaac
    11761 cgcgtttttg cccacctggc aataagtgag aatcgtagcg atcgctcaac agcggcgtgg
    11821 tccagtcctg aaacggcgtg ggtaacatct gtaacaacaa tggcgtggcg gcaaaggtca
    11881 tggttatcgg acataacgac cctgcctcaa cctgtgcgtg caacataaaa cgcgctgcgc
    11941 gcgccacaaa tgcgccggag cgagcgtctt cttcccaggc aagattgtgc acccggttgg
    12001 tacatagcgc ctgcatcagc aagtgccagg cggggtgaaa acgcacatcg tccagatggc
    12061 gtccttgcgc atcgtagcgc aataattcag gcggattcac attcgccagc cgccccagtt
    12121 caagagattc agccgttcct aactgctgac caatactggc tagaaaatcg ctatcccagc
    12181 cagcaccttc acgcgttacc gcttcacaga gcgcgccatc agacaggtat aagttactgt
    12241 tatttaatgg tataggttga ttaaaaacgg tgtgagtttg ccagtgcact gtgtctccct
    12301 ccatcaatgg cagtcaccct tagtatggtc actgccatgg attcctgaca gaaagagatt
    12361 gctaaaacat tcttatccag caatgtaatg cggaacgaaa cgtgaggtgt ctttggtgat
    12421 cagcgtgtta tcttcacgga tccccattcc gcacgcttca tcatcgacaa tccagctacc
    12481 gatgagtgtg tagctatcgc caaatcgcgg cagaggttga aacgcctggt agatcatcgg
    12541 ttcatcggcg taatcaccat cagcgtggtc aacgacgtta ttctgaccgt cgaaaatggt
    12601 gacattgccg ccttcgcgcg agtagatcgg tttacgcaca tagctttcgc cagcggcaat
    12661 ctgcgatttt tcaccctcaa accaggatgc aagaaggtta ggatgaccag ggaagaagcg
    12721 ccaaagcagt ggcattagcc ctttattact caagatactt ttccataacg gctccaccca
    12781 ttgctcgcga cgcttgcgca gcagcggacc gttatcgtca cgcatcatcc actctagcgg
    12841 atacagctta aatgcacgct ggatgacatt atcatcaaga tcggtcagta cgccgccgac
    12901 gcccaaaccg agatcttcaa tgtagataaa ccgcgactcc tgccctgcct gctgggcgca
    12961 gtcctgcaag tacagcacgg tactcctgtc ttcatcggtg tcctgacagc agcaaaaata
    13021 aaacggttcc cgactgtaaa gctcactaaa gcgcgaaatc aggcgttcct gaatagcatt
    13081 gtactgatcg gcatcacgcg gaataatgcc gctgcgccgg gcatcctcca gccacagcca
    13141 ctggaaataa gccgattcat acaacgaggt tggcgtatcg gcgttgtact ccagcagctt
    13201 caccggcgca ttgccacacc aggcaaaatc catccggcca tagagcgaag gatcgcgtgc
    13261 gcgccagctt tcggcgatca catcccagta caacggcggg atcgccagtt gcgtcaggat
    13321 ctcttcgtct ttaaccgcgc ggtccaccac ctcaaggcac atctgatgca gttccgcagt
    13381 tggtttttcg atctgctctt caatctgacg cagggtaaag cggtaagccc gactctcatc
    13441 ccaatagatt tcattgtcga tgatatgaaa gtcgaaaccg ttgtcagcgg cgatctggtc
    13501 cagatcccac cgtacaggaa cgttgtgtct cagcatgatt agcctcccca atgcccacgg
    13561 gcgcgggaag aacgaccata gccgccgcga gaaacggttg acgctttttt ggtggtaaag
    13621 ccgcccgaag aataggactc ttttttgcca gaaccggagc gccaggagta atcaccggaa
    13681 gtgttgcgcc agacggggcg cgaagcaaaa gaggaaccgc cgctgttata cacaaactgc
    13741 tcatcgcgat ctttacgaat aacccggctt aacaaaaatc cagaaacgac cgggatccaa
    13801 ctctgttcaa cgttgtcgta gtaacaattt tcatacttag actggcaatt ctgctgagtc
    13861 atattcttcg gaacatcggc ataaaacgcc gctttggcgt tgttccagcc atgagcacag
    13921 atatctgaat tattaccgtc atcaatacaa tcctgcacag tcgagtaaaa cgttccgtcg
    13981 ccgtcgttat cgacatcgcc actatcgcca caacctttca atacgaaaaa agcggcaccg
    14041 cccattagcg ccaacgttaa gtatttttgt gcgtaacgat tgcgacgcgg ttcaaaagga
    14101 ttattcggtc taccaatgcg actgatcgct ccgtgaccaa ttttactgtt atttcttgat
    14161 ttgcgttttc tggccatgat cgtccattac catgtcatac aagctgcgtt aaaaataccg
    14221 ccagccagtg cggcggttcc cataaacatt cctgctgcgg tattgtgatt aataattttt
    14281 tcgcttaatg cgggcatata aagtctgacg ccagcaaaaa cgagtaactg aatcaccagt
    14341 gcgatcccgc cccaggcgaa atagtctgga atactcaccg cattgattgc cgcactggat
    14401 aacgggatca cgtaacccaa caatgtaccg ctgaacgcca gggacgccgc ggtattattg
    14461 tttttgatta actgccattc gttgtgcggt gtaattttag aatagataaa cagaaaaata
    14521 atcaccatag ccacgccaat aaaaaaatag gcactaaagg caagaattga atccagtata
    14581 tgcattgtaa acgtccttgt aattaaccaa taatatgaag tgatgtcagc gggatatcga
    14641 cacccaacgc gcgcgaaaat aaccactctg gctcgccgag atcgttaaaa gattcctcac
    14701 catttagcag caaatattcg taagtatctt cggttacttg ccgttggtag cccatggtaa
    14761 aattatgtac ttcccatttg gcatggtttt gattttctac tttttccagc atgtagacgg
    14821 gttcgatatt tcccggttct tcgctgttaa aaaatcgctg ccagcgtttt tcctgccagt
    14881 ttaaggtcat tgcccccatc gctttggcat tgatcgtctc gcgccagtga ctctctttac
    14941 tgataccgta gctctcttca tagacaaaga gcttaatatc atcaatgtca tcaatatttt
    15001 cgccgccggt ggtattgatt tgcagaaatt catcgcccga agtgtagtag cggaaaatct
    15061 ggctaccgcc gcccagatcg atgtgactga cggcggctac cgtaaattcc tcacccggca
    15121 gcgcaatcag caattcatct tccagcaaac gaaacgctaa cgtgtcgagc gtaaaaccac
    15181 tattgagatg cagacccagc ggaccgcgag cgatagcagg cttattatcc ttgccaaaca
    15241 gacgctggaa aaaaccagac atattttatc cttaaaatag ttgccagcct tgcagtgagg
    15301 ctggcaaaaa aattactcgc cctgttggcg ttgcagtctt gctaatacat cctgggcgct
    15361 acttttattg ctaccgccta ttccggcttc cgccagcttt tcatcaagat cgcgaccgtc
    15421 tgcgactttt tccaactgtg cagcagcatc cagacgagcc tgacgttcgg cctgacgcgt
    15481 ttgcaggcgt tttaaggatt ctgccgctgt cgaaacgctg gaagatgcgc caacggtaga
    15541 ggtcgttaca gcctgttgtg cacgctgcat ggcttcagtg gctttaacga cttccatttg
    15601 ctgctcaaac tgagcgatac gctgcgctgt cgctgtaact gctttttcca cgccatcacg
    15661 agagacttcc agattcgaca acacttgctc ttcagcggta atgagattct caaggcgcgc
    15721 gatttcttca gcaacttcgt taatcaacga cggattaacg ttcttgctta acgctttcag
    15781 cgcacgagct tccagactgg ctttgcgctc acgtaaatct ttcagcttat cgtgacttaa
    15841 tttcacccgc gccagcagat caacgcgaga ttttccggct ttatcgagtt cagctttagc
    15901 gtcacgaata tgctgttcca gcatgcgcac gccctgggtt tcttcaatgg attcttccgc
    15961 ctgggagata aacgatttcc ccagcgtaaa taaacttttt aaaattccca taaatacccg
    16021 tccttgtaat tagtgtgaat attcttctgt aatttcagcc agatccaatg cgttatctac
    16081 cagcgaggtt atctcaagca ggatatcttc aagagaagat ttaagcgata acgccccgaa
    16141 aacaatgtaa tactcttcct gttgtacgct ggagatccct accgatgaca acggcatcat
    16201 cttctgattt cttaataaga aggtattaaa ttcatctgga ttgctgatac tactcaccgg
    16261 acaaataaag gtttcgataa tcatttggcg ggaagtaaaa agaatattga tttgcaaatc
    16321 gccataatca ttcattttaa taattaatgc gttctcgcta tttgttacat caatattttg
    16381 ttcaggtaca gtttgcagcg ccgtcgccag cgccaacgga ttccatgtca tatattattc
    16441 catatagatt tagtttaaat attaacaaaa tgaatatttg cagtacgtaa ttatcttacc
    16501 agctaaacac aaaaaaaacc atccaaatct ggatggcttt tcataattca gaggaactag
    16561 ctgcgctgtc gcaccgcttc aaataagcaa atccctgtcg caaccgaaac gttcagggaa
    16621 gaaacgcttc ctgccattgg gatgctgatc aactcgtcgc aatgttcacg agtcaggcga
    16681 cgcatacctt caccttccgc gcccatcacc agtgccagcc gtccggtcat tttgctctga
    16741 taaagtgtat gatccgcctc acctgccgta ccgacgatcc agatattctc ttcctgcaac
    16801 atacgcatgg tgcgcgccag attagtcacc cgaatcagcg gaacgctttc tgccgcacca
    16861 caggccactt ttttcgccgt ggcgttgagc tgtgcggagc gatctttcgg cacaatcacc
    16921 gcatgaacac cagcggcgtc cgcgctacgc aggcacgcgc cgaggttgtg cggatcggtt
    16981 acgccgtcga ggatcagcag gaacggttga ttgagcgaag cgatcagatc cggcagatcg
    17041 ttttcctgat actgccgtcc tggcttcaca cgggcgataa tgccctgatg cacggcaccg
    17101 tcgcttttct cgtcgagata ctgacggttc gccaactgga taaccacgcc ctgggactca
    17161 agggcgtgaa tcagcggtaa cagacgttta tcttcacggc cttttaaaat aaagacttcc
    17221 tgaaaacgtt caggggcgcg ctccagcagg gcctgcactg cgtggatgcc gtaaatcatt
    17281 tcgctcatta atgtactcgt tgtttacgtt ttgccgggag cgccacgctt gcccggccta
    17341 cggataattc accctcttct gtttgaagag ggttatgatc actctgccac ttttttcttc
    17401 gccgcacgct tcgctttggt cgctgcggct attttctgcg ttttagccga tggctttttc
    17461 gcttttctcg cgtctttctt cgccgctttc ggcttcggtt ttttctcgcc gcggaaggca
    17521 ctgtctggct caaagtttac ctttttaccg acctgacgac gcttgccgcc ttttttgcca
    17581 gcatcgcctt ttttcgcttt ctcgcgcgcc gttttaccga cgttgcgcgg tgcgcgttcg
    17641 ctggagatca ggctaaagtc gattttacgc tcgtccatat taaccgcttc gacgcgaact
    17701 tccacgcgat cgcccaggcg ataagtctgg ccgctggatt cacccatcag gcgttgccct
    17761 acctggtcaa agcgatagta gtcattgtcc agcgaagaga catggaccag accatcaatg
    17821 aacaagtcgt ccagacggac gaagaagcca aagccagtga cgctggaaat tacgccttta
    17881 aagacgttac ctacctggtc gagcatgaag tcacacttca gccagtcagc tacatcgcgt
    17941 gttgcttcgt cggcacgacg ttccgccatc gaacagtgct gacccagttg cagcatctct
    18001 tccatcgaat aatggtaacc accggtttca gtggtattgc cctgatgccc ctgctctttc
    18061 gccagcagat acttaatggc gcggtgcagc gtcaggtcag gataacgacg aatcggcgaa
    18121 gtgaagtgcg cataggactg caatgccaga ccgaagtgac cacggttttc cggatcgtaa
    18181 atcgcctgtt tcatcgagcg cagcagcatg gtttgcagca tttctgcatc aggacgatcg
    18241 gcaaccgact ccagcagctc cgcgtagtca cgcggttccg gcttgttacc gcccggcaat
    18301 tccagcccca gctccgccag cactgaacgg aaagaggtaa tcgcttcggt gctcggcttg
    18361 tcgtgaatac ggaacagtgc tggttctttg gctttctcaa cgaaacgcgc cgccgagata
    18421 ttcgccagaa tcatgcactc ttcaattaac ttgtgtgcgt cgttacgctg agtctgttcg
    18481 atacgctcaa tacggcgttc ggcgttgaaa atgaacttcg cttcttcgct ctcaaatgag
    18541 atcccgccac gttcttcacg ggctttatcc agcactttat agaggttatg caactcttcg
    18601 agatgcttaa ccagcggggc gtactgctcg cgcagatcct gatcgccctg cagaatatgc
    18661 cagactttgg tgtaggtcag acgtgcgtga gagctcatta ccgcttcata gaatttgtag
    18721 ccagtcaggc ggcctttcga cgaaaccgtc atctcgcaca ccatacacag gcggtctacc
    18781 tgcgggttaa gcgaacacag accgttagag agcacttccg gcagcatcgg gataacctgc
    18841 gaagggaagt acaccgacgt accacggtta cgcgcttctc tgtccagcgg tgttggcgga
    18901 cgcacatagt agctgacgtc ggcaatcgcg acccataaac gccagccgcc gccgcgtttt
    18961 ttctcgcagt aaactgcatc gtcaaagtca cgggcgtctt cgccatcaat ggtgaccagc
    19021 ggtaaatcgc gcagatcaac acggcccgct tttgcttctt ccggcacttc ttctttcagc
    19081 ccggcaacct gttgctcaac agcctgcggc cagatgtacg gaatttcatg ggtacgcaga
    19141 gcgatatcaa ccgccatgcc ggtgcccata ttgtcgccaa gtacttcgac gattttaccc
    19201 accgctttgg tacggcgagt cgggcgttgg gtcagttcaa ccaccaccac aaagcccatc
    19261 cgcgcgccca tgatctgatc gggcgggatt aagatatcga agctcaaacg actgtcgtca
    19321 ggaaccacaa agccgacgcc cgcttcggta aagtagcgac caacaatctg gctggttttt
    19381 ggcaccagta cgcggacaat acgcgcttca cgacgacctt tacggtcagc gcccagcggt
    19441 tgcgccagca cctgatcgcc atgaatgcag gttttcatct gctcgctgga gagatacaaa
    19501 tcatctttac gtccttcaac ccgtagaaag ccgtagccat cacggtggcc aataacggta
    19561 cctttcacca ggtcgaggcg ttccggcagc gcatagcact gacgacgagt gaagaccagt
    19621 tgaccatcgc gctccatcgc gcgcaggcgg cgacgcaggc cttcaagctg ctcttcgcct
    19681 tcaatgtgca gttctaccgc cagctcatca cggctggccg gtttttcacg tttggttaaa
    19741 tgttcgagga taaattcccg actagggatg ggattcgcgt atttttcagc ttcgcgttcc
    19801 tggaaaggat cttgtgacat ctcggttcct ccgttgtcat ctctgatgaa gattttcgtc
    19861 actccaccag caataattta taaagcggtt gattctcttc aaccaaatcg gcaagcgtgt
    19921 agttatccag ttccgtaaga aaactttgca cggccttaga aagtgcctgt ttcaaccgac
    19981 aggcaggtgt aatgtggcaa aactcactgc tgcaattcac cagcgataag ggctccagct
    20041 cgcgcaccac atcaccaata cgtatcgcac tcgccggttt acccagacga atgccgccat
    20101 tttttccacg aacagcagtc acgtagccgg cacgactaag ttgattgatt attttgacca
    20161 tatgattacg ggagacgccg tagacgtcag tcacttcaga aatactggtc atccgccctt
    20221 ctggcaatga cgccatgtag atcagcgcac gtaatccgta atcagtgaaa ctcgttaact
    20281 gcacatcaac ctcaaaaggg aaatcgggaa aaaatacatt tatattgatg ataaaccagc
    20341 cacaagctgt gtcgctaatt tatttcagtt cggggaagga aaaaagcgag gatttaacac
    20401 tgtgtcggat agcgtaatgg cttacccgac acagcaatgt atcacactgt tagcccggca
    20461 ggcaaaatat ctgccaggcg taccagaatt acgcgtcgaa cgggtcgcgc agaatcatgg
    20521 tttcagtacg atccggaccg gtagagatga tatcgatcgg cacaccggtc agctcttcaa
    20581 tacgcttgat gtagttcagc gccgcctgcg gcaggccgct acgatctttc acgccgaagg
    20641 tggattcaga ccagcccggc atggtttcgt aaatcggctc tacacctttc cagtcgtcag
    20701 ctgccagcgg agtggtagtc acttcacgac catccggcat acggtaagcc acgcagagtt
    20761 tcacctcttt caggccatcc agaacgtcca gcttggtcag gcagaagcca gacagggagt
    20821 tcagctgtac cgcacgacga acggcaacgg tgtccagcca gccggtacga cgacgacgcc
    20881 ccgtagttgc gccgaattcg ttaccctgct tgcagaggaa ctcgccagtt tcatcaaaca
    20941 gttcagtcgg gaacggacct gcacccacac gagtggagta agctttgagg atacccagaa
    21001 cgtaatcaac ataacgcggg cccaggccgg aaccggtcgc cacgccacca gcagtggtgt
    21061 tggaagatgt tacgtacgga taagtaccgt ggtcgatatc cagcagcgta ccctgagcac
    21121 cttcgaacat gacgaaatcg ccacgctgac gcgcctggtc gagcaggtca gaaacgtcaa
    21181 ccaccataga agtcaggatg tcggcaacag ccatcgtatc atccagaact ttctggtaat
    21241 caaccgcttc agctttgtag tagttaacca actggaagtt gtgatattcc atcacttctt
    21301 tcagtttttc agcgaaggtt tctttgtcga aaaggtcgcc aacacgcaga ccgcgacgtg
    21361 ccactttatc ttcataagca ggcccgatac cacgaccggt ggtgccgatc gctttcgcgc
    21421 cacgcgcttt ctcacgcgcg ttatccagcg caacgtgata atcaaggatc agcggacatg
    21481 cttcagacag cagcagacgc tcacgaacgg ggatgccacg gtcttccagt tctttcatct
    21541 ctttcatcag cgcggctgga gacagcacaa caccgttacc gatgatgctg gttacattct
    21601 cgcggagaat acctgatgga ataagatgga gaacggtttt ttcaccgttg attacgagag
    21661 tatggcctgc gttgtgaccg ccctggtagc gtacaacata tttagcccgt tcagtcagaa
    21721 gatcgacgat cttaccttta ccttcgtcac cccattgggt gcccagtacg acgacgttgt
    21781 tacccatttt tcaaaatcac cgtttgctta aaaatggatt ctaccatcgc tttttcagag
    21841 ttacagcact ttttgcactc aaaaatgacc tcgatcagtc tgttttttgt tcagccaatc
    21901 gttttcctca acatgtagta gaccacaacg cccgcaacca caagtccacc gccaaaacga
    21961 cgtaaaatat tatcgggcaa attggtcatc gcagagatca tcttcttcca tgccttcggg
    22021 taaagcatcg gccctaaacc ttccagtacc aaaaccaggg caagcgccag ccagattgtc
    22081 gaattcattc attatcctta tagaaaaaga aaaccaccga catccctgag gatgcggtgg
    22141 ctttattgac ctgtaccgca gtcgttatat taacgcgttg cggaagtcgg cgtcttcatg
    22201 tagcggaaga aatcgctatc cgggctcatg accatcacgt cctgattgcc agagaagctg
    22261 ttctcataag cacgcaggct acggataaat gcgtagaagt ccggatcttt actgaatgca
    22321 tcagcaaaca gtttggctgc ttctgcatcg ccttcaccac gcatgatgcg gccctgacgc
    22381 tcagcttctg ccagcgttct ggtcacttca tagtcggcag tcgcacgcag tttttccgct
    22441 tcttcctgac cttgtgaacg gtgacgacgc gctaccgctt cacgctcggc gcgcatacgg
    22501 ttgtagatcg cttcagacac ttcggtcggc aggttgatct gcttaatacg cacatcgaca
    22561 acttcaatac ccagcgccgc catactgttc gggttgatga ccggaacttt acccttcgtc
    22621 tcagccgtta cgcgctctgc cgcttcggca atggcgttat ctgctgccgg ggtagtaact
    22681 tcatcttctg tacccgcaga accggagttc agcgcgtcac gtacttcgag ggtcagacga
    22741 ccacgggaat cggtgacgat gtctttcacg tccaggcgac caatttcaga acgcagacgg
    22801 tcagagaact tacgtttcaa cagcacttcc gcttgcgaaa tgtcgccacc acccgttgcc
    22861 aggtagtaac ggctgaaatc gctgatgcgc catttgatgt aagagtcgac gatcaggtct
    22921 ttcttctctt tggtcacaaa gcggtcggcc tggttgtcca tggtctgaat acgtgcgtcg
    22981 agcattttca ccgtttcaat gaacggtatc ttgaaatgca gacccggctc ataaaccaga
    23041 ggtttgttgt catcgtcacg cagtacctta ccaaaacgca gcgtaatacc gcgttcacct
    23101 tctttgacga caaagacaga catgtaaagc actaccagca cgatgatgat aatcgcgata
    23161 actgacttac gcatcgttat tccccctgac gctggtagtc gttacgctgc gcgttggcgc
    23221 ggcgttggtc cataatatcg ccctgactgg tggacgacgt gttgcttgct ccactggttg
    23281 tggtggaaga ggctggcggc agacgcagca gattgctggc accgttatcg ctcttcgccg
    23341 caggggcgtt accacctttc agcatctggt ctaatggcag aaccatcagg ttgccacctt
    23401 tatcgttaac cagcactttg cgggtgttgc ccaacacttt ttccatcgtc tcgatataca
    23461 gacgctcgcg agtaatttcc ggcgcggctt tatattccgg cagaagttta gcaaagcgcg
    23521 ccacttcacc ctgagcttcc aggatggtct gggccttgta cgcacgcgcc tcttcgagga
    23581 tacgttgcgc ctgaccgttc gcacgcggct gaacttcgtt ggtatacgct tctgcttcac
    23641 gaatgtattg ctgttcgttt tcacgcgcgg caatcgcatc gtcaaacgcc gcttttactt
    23701 cttccggcgg acgagcagcc tggaagttga cgtccagcag cgtgataccc atgtcatacg
    23761 gacgaatcgt ctcttccagt tcgcgctgag tatcgctacg aatcacggta cgaccttccg
    23821 tcagaatgcg gtccatggtg tatttaccga taaccccgcg tagggcgctg tcggtagcct
    23881 gacgcaggct gtcatccggg ctggtcacgc tatacagata tttttccgga tcggtgacgc
    23941 ggtactgcac gttcatctca acgcgcacta cgttctcgtc cgacgtcagc atcacaccag
    24001 aagctgccag ttcacgcacg gcttccacgt tcaccggttt gacttcgtca ataaacgtcg
    24061 gtttccagtt cagacccggc tcaaccagat ggctgaattt accaaagcgt gttaccacgc
    24121 cacgttcggc ttctttaatg gtatagaaac cactggccgc ccagataatg acaatcgctg
    24181 ccgctgcgat ggtaacgaca cgaccgccaa gctgcgggcg cgggccttgc gatgaactgc
    24241 caccgccaga tccggtgcct ttaccgccgc ccagaccacc gagctttttg ctcagcttgc
    24301 ggaagatatc atctaaatca ggtggccctt gatcgcgacc gcctttgttt ccatttccct
    24361 cagagttgcc gccaggtttg ctgcttcccc acgggtcgcg gtcttgtccg ttattaccgg
    24421 gctgattcca cgccatgttt gtgctccata tttgttatgc ggtgatccct gttgtctttc
    24481 gacgccagag gatatgactc cacgcttcag acgctacgcc gttagatcag gtaatcgatc
    24541 aacgccggtt cttgtttaca gaggcgacgc cagtcaacga tcggcatacg aacttgcaga
    24601 cttacgctgc cgtcctcctc catccactct ttttctattg cctgaagctg ataaaaacga
    24661 cttctcagac gcccttcctg cggtggcaga cgcaatgtat gctgcgccac ctcgccggaa
    24721 agccgctccg tcaaagcctg aaaaagctgt ggtatccccg ctccggtctg tgcggaaagc
    24781 cagacacgga tcggtttgtt ctcttcgtcc cgatcaatac gcggttcgaa atcttccagc
    24841 atatcgatct tgttcatcac cagcagggtt gggatctcgt gagcgtcgat ctcttcaaga
    24901 accgtattca ccgcttcgat gttttcttgt acacgtacat ccgccgcatc aatgacgtgc
    24961 agcagcaatg tggcttgccg cgtctcttgt aacgtggctt taaatgccgc caccagatcg
    25021 tgcggcaggt ggcgaataaa ccctacggta tctgccaaca cggtttcacc gacatctgcg
    25081 acgtcaatac gccgcaacgt cgggtcgagg gtggcaaaca actggtctgc cgcgtagacc
    25141 cgcgcttcgg tgatgcgatt gaaaagggta gatttaccgg cgttggtata tcccaccagc
    25201 gaaacagtag gaacgtcggc tttgatacgc gattgccgcc cctgctcacg ctgcttttca
    25261 actctttcca ggcgcgactg tatctgcacg atgcgattac gcaacaaacg acggtcggtt
    25321 tcgagctggg tttcacccgg accacgcaaa cctatcccgc ctttctgtct ttcaaggtgg
    25381 gtccagccac gcaccaggcg cgtagccaga tggcgcagct gcgccagctc aacctgcaac
    25441 ttaccctcat gggtacgggc acgttgggcg aaaatatcta aaataaggcc ggtgcggtcg
    25501 atgacacgac actcgcacaa acgctccagg ttacgctctt gcgccgggct cagggcatgg
    25561 tcaaaaagaa cgaccgaagc acccgtcgct ttgacagctt ccgcaatttc aactgcttta
    25621 ccttcaccta caaaatactt tgggtgcggc gctttacggc taccggtaat cacctgcaat
    25681 gcttcgacac cggcggaaga gaccagagat tcaaactcct ggaggtcttc catatctttg
    25741 tcttgcgtaa aatagatgtg taccagtacc gcctgctcac cagcatcata acggtcaaac
    25801 aagcgtataa ccctctaaat agatcagcgg ggaacgcagg atcgctggct ccccgtgtaa
    25861 aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga agtattctgc
    25921 gcgctgctac catgatggta gttactgctg gtaccgccac cggcgttgtt actgtgatga
    25981 gaaaccgggc gagacgggac aacagtagaa atcgcgtgct tataaaccat ctggctgacc
    26041 gtgtttttca acaggatcac gaactgatca aaagactcga tttgtccttg cagcttaata
    26101 ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt caggaacgga
    26161 tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat ttgtactttg
    26221 aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca ttcagcctgc
    26281 gatagcacca acaacctgta atacttcgtc acgcgcttgt tctggttttt cactgtcaag
    26341 ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca actgtctcgt
    26401 ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgccttcaa ggtaagacca
    26461 catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat ctcctcgggc
    26521 aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa aacgctgctc
    26581 aattcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat gcacctgata
    26641 cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg aaatgaaaaa
    26701 aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg ctgccgcaac
    26761 cggatcgatc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg ccgcctgttg
    26821 ctcaattctg gcccgcactt ccgggtctgc cgacggtagc ggcgacaacc cttccagcaa
    26881 tgccttgaaa tacaacatag taccgcccac taacagtggg atccgccccg ccgcggtgat
    26941 atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag cctgcgacgg
    27001 atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt tcggcttcgc
    27061 cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca actctactgg
    27121 taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg gccccatcaa
    27181 aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg gcttttatcg
    27241 ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca agttgcggac
    27301 ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac tgcgcatgtt
    27361 cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat acggactgct
    27421 tcgccaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg cgtaagggta
    27481 aaggcacggc cctgatggtc acatgctgcg catcggactg gaaatcaata cccaattccg
    27541 ccagggcaga ctgcgctttt tctaatgccg atttttcttc accagaaact tttagccgca
    27601 gcggaatcag caacggctgg gcgcaaactg gcgcttcacc cggcgtcagt tgcgcctgac
    27661 gcagccaacg ttctgccact ggcaaggcta aaagtgaaat gttgccgtcg cgctccagca
    27721 acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg ttcgccgcaa
    27781 gtgcaggttc ctgcggttct ggcgcttttg gtttttgcat cggcgcgggc gtttgcaaaa
    27841 gctggcgata cacttcacct tgctgtttct ggtaacctgg ctgcgcattc ggccaagggg
    27901 cagccggacg actgcctgag gcgggcgcag gagtgtaacg cggagctact ggctcacgaa
    27961 ctgccggttc agcaaaatga ttgcgccccg ccgccacgcg gttttccggg atggcgcgcg
    28021 gtgcaggttg tggttcatcg tccagcggta gcggcgtttc cagttgctgt tgtagcacgc
    28081 tcagcacgcc ctgatagata aaatcatgca ccagacgcga ctgatggaaa cgcacttcgt
    28141 gtttggcagg gtgcacgttg acgtccacct gatgtgggtc gatctccaga tacagcacaa
    28201 atgccggttg ctgatcggcc cccagtttgt cttcacaggc ctggcggatc gcgtgattga
    28261 tcaggcgatc gcgcatcatg cgaccattca catagcaata ctgaatttcc gccagtgcgg
    28321 gcgtggtgtg attcggatct gccacccagc cgcgcagcgt aagatcgccg tgttgccatt
    28381 caatcgccag cgcttgttcg agaaaagcgg tgccgcaaat cgcgcctaag cgccgttctt
    28441 tttgcccgcc ttccggcact gcgcggtact gacgcacaat tttaccgtta tgcgacaggt
    28501 tgatcgtgac gtcgaaacgc gccagcgcaa tgcggcgaat aatttcatcg atatggttaa
    28561 attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag aacagatcca
    28621 gcacctccag cgtcgttccc accggatgcg ccgccggttt aaccgttacg tccatatcgc
    28681 gcccttcggc ataggcctgc caggcttcct gctgttctgc gatgcgtgaa gtgagcgtca
    28741 ggcgcgaaac cgaactgata ctcgccagcg cctcaccgcg aaagccaagg ctgataatgg
    28801 cttcgagatc gtccagagag gcgattttac tggtggcatg acgagccagc gccagcgcca
    28861 gctcatcttt tttgataccg cagccgttat cacgaatgcg gataagtttc gccccaccgc
    28921 gttcgatatc aatatcgata cgcgtcgcac ctgcatcgag gctgttttcc accagttctt
    28981 tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc gccagttgtg
    29041 gcggtaagac ctgaattggc atcaataatc cttagtttgg cagcgtgcga tctggcgtcg
    29101 tcaccgtact ggccgtttgt gccgctgcac cctgcggcgc agattgcatc ggatgcgcaa
    29161 ggaaataatt gcgcaggcct ttgtaaatgg cttctgccag ctgttgttgg taatcgtcgc
    29221 tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc agtactgacg
    29281 ggatatccgg cgaacgcaga acaccaaggc tggcatgttc tggccgacgt ttatgtattt
    29341 cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct acccgctggg
    29401 aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata ggggtcagac tgactgttcg
    29461 ccagcacatc gcccgctccg cccaatagct ccgactgttt ctcatgctgt tccagccagc
    29521 ttgccatctc gctgtttgca cgacggttag agagcaccca tacggaagcg ccagtcgcac
    29581 tgcggtttgg tgcggcatca gcgtgaatcg acacgaggaa attggcgttt tgcttacgtg
    29641 ccacatcgct gcgccccatc accgaaataa agtaatcccc atcacgagtt aaaacgcctt
    29701 taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg atggtgacat
    29761 ttttctcccg cgtaccaccg gggccgatag cgccaggatc ctgaccgccg tgtccggcat
    29821 caatagcgat gataatttta tcgcctgtgt tagccgtcgc acgcgctgcc ggacgcgtta
    29881 ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac ggattgcgcg
    29941 ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc aacgcgtttc gcaaccacag
    30001 gcggcggagg aggcggtggc ggcgcatcgg cgttaatcgt aaagacgaca gtgtaattgc
    30061 tgccattctg ccgcttcacc gcttcggttt taccattttc ggtaagatcg accaccagcc
    30121 gtagcgtttg tgcatcttta ggcgttccag agcgaatcgc cttcaccaga ttattgccgc
    30181 tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc gccacggtgc
    30241 gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac gttatccgcg
    30301 cctgttggtt gccgttagaa acctgaatat cagagagcgt cgcggcaccc accggcgcgc
    30361 acagcagcag caacgtcgct accaaccaat ttctgatgcg atacatcatc ccgccacctt
    30421 tcaaaggtta accggctaaa cgcgccagca acaattcacc cgcagaggaa accgcactca
    30481 cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc gggtcaggaa
    30541 gaacacctgt accttgttgt ggccactcca ccaggcagat ggcatcgttg gcaaaataat
    30601 cgcggatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa tcaaagtgat
    30661 aaaccattaa gttgtcgagc gtatagggtt cgaccagcgt ataagtgggg cttttgacat
    30721 taccctgatg gcccaaagcc tgcaaaaagc cccggctaaa ggtggtttta cctgcgccta
    30781 aatcgccata cagatagatt acggttgcgc catcgcaggc tttcgctacc cgctcgccca
    30841 ggtctaatgt tgcctgctca tcagggagcg gaattactcg attcatcatg gtttttatca
    30901 gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc cagcatcccg
    30961 cgcgttccaa aacgcgccgc cagtacgtca gctgccgcac cgtgcgcgac acagcccgca
    31021 caggctgcat catacggcga cagtttttgc ccaagcaatg cgccaataat accagagagc
    31081 acatcgccca tgccgccgct cgccatgcct gcattcccgg catcaataat gcctaaagcg
    31141 tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc gccataacgt
    31201 tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac ggaacagcct
    31261 aacaaccgtg cggcctcgcc aggatgtggc gtgatcacgc gattgtgacg cttatcggga
    31321 ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg aaaattctca
    31381 actttttgca gggctttttt cccccactct tgctggccca gaccgggacc aatcaccacc
    31441 acatcggccc atcccaggct ttcggtaaga gagtccatag tcagttcatg caccatcaat
    31501 tccggtcgtg cagtcagtag cggcgcaatg ttttcactgc gggtcaatac tcgaactaaa
    31561 ccagcaccag cacgcagtgc cgcttccccc gtcatacgaa tagcccccgc cgtgccgtga
    31621 tcgccaccga taatcaccag ccgcccgtga tcgcctttat gcgatgtcgg gcgacgcggt
    31681 ttcagccagt gagaaagttg ttctgccgaa aaccgctgaa ttttcgtctc ctgacctgcc
    31741 agccagctat ccagccccag tgagtcaaaa tgcagttgtc cggtaacatc ccgcgctttt
    31801 ccagtgagca agcctggttt cagcgcaata aaagtgatgg tgtgatcggc gttgatcacc
    31861 gcgcctggcg tagcgccagt ttcagccagc aggccggaag ggatatcaac cgccacaatc
    31921 ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg gggtgcttgc
    31981 tgcaagccgg taccgagcag cgcatcaaca atcagatcta ccgattcggg ccagacaata
    32041 ttcgaagcat ggatctcgcc tcccgcgttt aaccatgctt cgcgtgccag cgcggcctct
    32101 tccggcaacg gtttgtcact ttcctgggcc agcagcgtga cctcaatacc gaccgctttg
    32161 gccagtcgcg cgaccacgta gccatcgccg ccgttattac catgaccgca cagcaccagc
    32221 cagtggcggg cgtcaggata agccgtgcga cacacctgga atgcggcttc gccagcgcga
    32281 agcatcagct catagagtgt gagtcccagc gcatctgccg cctcgcgttc tccgcggcgg
    32341 atatcgtcgg cgtaccagac ggtgtgtggt atacttacgg ggtttttctt cattgtatgg
    32401 tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa acagtggggg
    32461 ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcgc gtccgagccc
    32521 aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat ggcgcgtcac
    32581 ggtatgctgc gtgctcgtcc tcacgaatta ttgcccggta cgctgcgcgt gatcagcgtg
    32641 cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa tcccaaactc
    32701 ggctatgtta gccgttatgc gctgggccgt gactatcaca agcttctgcg caaccgactc
    32761 aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag accgtttgtc
    32821 gattctgcgc ctattctcga gcgcccgtta gctgaaaaag caggacttgg ctggacaggt
    32881 aagcactcac ttatcctcaa tcgcgaggcc ggttcattct tctttttagg cgaattgctg
    32941 gtcgatattc ctctgcccgt ggatcaacca gtcgaggaag gatgcggtaa atgcgtggcc
    33001 tgtatgacga tttgcccgac cggagccatc gtcgagccat ataccgtcga tgctcgccgc
    33061 tgtatctctt atctcaccat cgaactggag ggggcgatcc cggaagagtt gcgaccgtta
    33121 atgggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg gaatcgctat
    33181 tcgcaactca ctacagaaga cgatttcagc ccgcgtaagc cgctacacgc accggaactc
    33241 attgagttat tcgcctggag tgaagagaag tttttaaaag tcacggaagg ttcggcgatt
    33301 cgccgtatag gtcacctgcg ttggctgcgt aatatcgccg tagcattagg caatgccccc
    33361 tgggatgaaa cgattttgac agcgctggaa agtcgtaaag gtgagcaccc acttctcgat
    33421 gagcacatag cgtgggcgat tgcgcagcaa atcgagaggc gaaatgcgtg cgtggtcgaa
    33481 gtgcaattac cgaaaaaaca gcgtctggtt cgggtgattg aaaaagggtt accgcgtgac
    33541 gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc aagccggaaa
    33601 aaatcgtcaa gtgatcgata cgacaaaacg aaatcttaat ttttaactta tctatcaata
    33661 ggttaaaaaa atactattca ccgtgcgaag tttctttgaa attaagatta cagcctgaga
    33721 ctgtggataa ctctgtttac aagagtttgt cagagacaac aaaaaccagc aataacgcat
    33781 cgcgtcgctg tggatatttt attgagagaa gaatttggag cgggaaacga gactcgaact
    33841 cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg cttgggtggt
    33901 ctgtgcttta cagcactttc aaattttgga gcgggaaacg agactcgaac tcgcgacccc
    33961 gaccttggca aggtcgtgct ctaccaactg agctattccc gcttaatctt cgtctttcga
    34021 accgctactg ccttggctgc cttcactcac cttagtcact tacttttgta agctcccaag
    34081 gattcgttca gttgccgcct tgtcgcaatt cgaaatactg cgattgggtg gtctgtgctt
    34141 tgcagcactt ttcaaatttt ggagcgggaa acgagactcg aactcgcgac cccgaccttg
    34201 gcaaggtcgt gctctaccaa ctgagctatt cccgcgtact acttaatttt gctttcgtaa
    34261 ttttcgcatt tctgcgtcgt tacgggaggc gcattatacg agaaatcttt ccctctgcaa
    34321 cccccctaaa aaattttttt tgagttttta tctcaagtgc tgaaattatc agcaaggcgt
    34381 tcaatttagc gtcaaaatca cgccaccgca agtggattcc gacgcttacg tgaccaggaa
    34441 ataatttaca gcttgataaa atgctcgcgg tagtaagcca gctccgccac cgattcacga
    34501 atatcatcca tcgcctgatg cgtcccctgc ttggtaaaac catcaagaat ttccggcttc
    34561 cagcggcgcg ccagctcttt cagggtgctg acatcaagat aacggtagtg gaagtaggct
    34621 tccagctccg gcatgtattt aaacaggaag cgacgatcct ggccgatgct gttaccgcaa
    34681 atcggcgatt ttcccgcagg cacccactgt tttaaaaatt cgagtgttgc cagttcagct
    34741 tcacgatcgc ccatcgtgct cgctttcacg cgctctacca gcccgctggc ggtatgggtg
    34801 cgcacgttcc agtcatccat cagcgccagc tgttcatcag actggtgtac tgcaatggtc
    34861 ggcccttctg ccagaatatt caggttggca tcggtcacca gcgtggcaat ctcaataatg
    34921 cgatcgcgct cgggatccag accggtcatc tcaagatcga tccaaatcag gttgttttca
    34981 ttggcactca tgctattttc cacccattca cagtgactat attgatccac aattgcgtgt
    35041 atcatagatg ttttgcccat caggggcgac caggagtctg tacgattgag taaaaataaa
    35101 ctctccaaag gccagcagcg ccgcgtgaac gccaatcacc agcgtcgtct taaaacgtct
    35161 aaggagaagc ccgactacga cgacaatctg tttggcgagc ctgatgaagg tatcgtcatc
    35221 agccgctttg gtatgcacgc tgatgtggaa tccgcagatg gcgacgttca ccgctgcaat
    35281 attcgccgta ccatccgttc gctggtgacc ggcgaccgcg tagtctggcg tccgggtaaa
    35341 ccggcggcgg aaggcgtaaa tgtcaaaggg atcgtggaag cggtgcatga gcgcacctcg
    35401 gtgttgacgc gtccggattt ctacgacggc gtgaaaccta ttgccgccaa catcgatcag
    35461 attgtcattg tctccgccat tttgccggag ctgtcgctca atattatcga ccgttacctg
    35521 gtggcctgcg aaaccttaca gattgagccg attattgtgc tcaacaagat agacctgctg
    35581 gacgacgaag gtatggagtt cgtcaacgag cagatggata tctaccgcaa tatcggttat
    35641 cgcgtattga tggtttccag ccatactcag gatgggctga aaccgctgga agaggcgttg
    35701 accgggcgca tcagcatttt tgccggccag tctggcgttg gtaaatccag cctgctgaac
    35761 gcgttgctgg ggctgcaaaa agagatcctg accaacgatg tctctgacaa ctcggggctc
    35821 ggtcagcaca ccacgaccgc cgctcggctg tatcacttcc cgcacggcgg tgatgtgatt
    35881 gactccccag gagtgcgtga gttcggcctc tggcacctgg agccggaaca aatcactcag
    35941 ggctttgtcg aattccatga ctacttaggt ctgtgtaaat atcgcgattg caaacacgat
    36001 accgatccgg gctgcgctat ccgggaagcg gttgaggaag gaaaaatcgc ggaaacccgt
    36061 ttcgaaaact atcaccgtat tctggaaagc atggcgcagg taaaaacgcg taaaaacttt
    36121 tctgatacgg atgactgaca attaagctaa ccgtcgttag aatcgtcccc tttttttcag
    36181 gatccggcat gtaggccgga tcaggaacga caaaacaatg gcctggaggc taccttgtta
    36241 aattcattta aactttcgct acagtacatt ctgccgaaac tatggcttac tcgcctggcg
    36301 ggttggggcg caagcaagcg ggcaggatgg ctgacaaaac tggttatcga tctgttcgtt
    36361 aaatactaca aggtcgacat gaaagaggcg caaaagccgg ataccgccag ctaccgcacc
    36421 tttaacgaat tctttgtccg tccgctgcgt gacgaagtac gcccaatcga taccgatccg
    36481 aatgtcctgg ttatgcctgc cgatggcgtt atcagccagt tgggtaaaat cgaagaagat
    36541 aaaatcctgc aagccaaagg ccacaactac agcctcgaag ccctgctggc aggcaactat
    36601 ctgatggcgg acctgttccg caacggtacg tttgtgacca cttacctctc cccgcgtgac
    36661 taccaccgcg tacacatgcc gtgcaacggt attctgcgtg agatgatcta cgtgccaggc
    36721 gatctcttct ccgttaacca tctcaccgct cagaacgtgc cgaatctgtt tgcccgtaac
    36781 gaacgcgtga tctgcctgtt cgataccgaa tttggcccaa tggcgcagat tctggtcggg
    36841 gcgacgattg ttggcagcat tgagacggtc tgggcgggca ccattacgcc gccgcgcgaa
    36901 ggcatcatca agcgctggac ctggcctgcc ggggaaaacg acggttctgt ggcactgctg
    36961 aaaggccaag aaatgggccg ctttaaactt ggctctaccg ttatcaacct gtttgcacca
    37021 ggtaaagtga atctggttga gcaactggaa agcctgtctg ttacgaaaat tggtcagccg
    37081 ctggcagtat ctaccgaaac ctttgttacg ccagacgctg aacccgcccc gcttcctgct
    37141 gaagagatcg aagcagaaca cgacgccagc ccattggttg acgacaaaaa agaccaggtc
    37201 taatccatca aaggaaacgc tgacgtgcgc ctgattatca cttttctgat ggcctggtgc
    37261 ctcagttggg gggcgtacgc cgcgacggcc cccgatagca aacaaatctc acaagaactg
    37321 gagcaggcaa aagcggcgaa acccgcgcaa ccggaagtcg tagaggcgct ccagtctgcc
    37381 ttaaatgcgc ttgaggaacg gaaaggttcc cttgagcgca tcaagcaata tcaagaagtc
    37441 attgataatt atccgaaact ctccgctact ctgcgcgcgc aattaaacaa catgcgtgac
    37501 gagccgcgca gcgtgtcacc gggaatgtct accgacgcgc tgaatcagga aattctccag
    37561 gtcagcagcc agttgctgga taaaagccgt caggcccagc aagagcagga acgcgcccgc
    37621 gagattgccg attcgctgaa tcaactgccg caacagcaaa ccgatgcccg ccgtcagtta
    37681 aatgaaatcg agcgccgcct ggggacgctt accggcaatt ccccgctcaa tcaggcacaa
    37741 aatttcgcgt tgcagtctga ctccgcacgc ctgaaagcgc tcgtcgatga actggaactg
    37801 gcgcagctgt ccgccaataa ccgccaggaa ttagcgcgct tacgctcaga gctggcggaa
    37861 aaagagagcc agcaactgga tgcgtatttg caggccttac gtaatcagtt gaacagccaa
    37921 cgtcagcttg aggcggagcg ggcgctggaa agtaccgaat tgctggcaga aaacagcgcc
    37981 gatttgccga aagatatcgt cgcgcaattc aaaattaacc gcgaactatc ggcggcgctg
    38041 aatcaacagg cgcagcggat ggatctcgtt gcctcgcaac agcgtcaggc tgccagccag
    38101 acgttacagg tccggcaggc gttgaatacg ctgcgtgaac agtcgcaatg gctgggatcg
    38161 tccaacctgc tcggtgaagc gttgcgggcg caggtggcac ggctaccgga aatgccaaaa
    38221 ccacaacagc ttgataccga aatggcgcag ttgcgtgtgc aacggttacg ttatgaggat
    38281 ctgctaaata aacagccgct gctaaggcaa atccatcagg ccgacggtca gccgctgacc
    38341 gccgagcaaa accgtattct ggaagcacag ctacgcactc agcgtgagtt gctgaactca
    38401 ttgttgcagg gtggcgacac gctactgctg gaactgacca agctgaaagt ctccaacggg
    38461 caactggagg atgcgctgaa agaggtgaac gaagcgacgc accgctatct gttctggacc
    38521 tctgacgtgc gcccgatgac catcgcctgg ccgctggaga tcgcccagga tctgcgtcgt
    38581 ctcatttcgc tggacacctt cagtcagttg ggcaaagcca gtgtgatgat gctgaccagc
    38641 aaagagacaa ttttgccgct gtttggcgcg ttgattctgg tcggttgcag tatttactcg
    38701 cgccgctatt tcacccgttt tcttgaacgt tcggcggcga aagtcggcaa agtgactcag
    38761 gatcactttt ggctgacgtt gcgcactctt ttctggtcga ttctcgtcgc gtcaccgctc
    38821 ccggtgctgt ggatgacgct gggttacggc ttgcgcgagg cgtggcctta tccgctggcg
    38881 gtagcgattg gtgatggcgt aacagccacc gtaccgctgc tatgggtagt gatgatttgc
    38941 gctacctttg cccgcccgaa cggcttgttt atcgctcatt ttggctggcc gcgcgaacgg
    39001 gtttcccgtg ggatgcgcta ctacctgatg agcattgggc ttatcgtgcc gctgattatg
    39061 gcgctgatga tgttcgataa tctcgacgac cgtgaattct ccggttcgct gggacggctt
    39121 tgctttatcc tcatttgcgg tgcactggca gtagtcaccc tcagtctgaa aaaggccggg
    39181 atcccgctgt atctcaacaa agaaggcagc ggcgacaaca ttaccaacca tatgctgtgg
    39241 aacatgatga ttggcgcacc gctggttgcc attctggcgt cggcggtggg ttatctggca
    39301 acggcgcagg cgctgttagc gaggcttgaa acctcggttg ccatctggtt cctgctactg
    39361 gtggtttatc acgttatccg ccgctggatg ctgatccagc gacgcagact ggcgtttgac
    39421 cgggcgaagc atcgccgggc agagatgtta gcgcaacgtg cgcgtggcga agaggaagcg
    39481 catcatcaca gtagccccga aggggcaatt gaagtcgatg aaagcgaagt cgatctcgat
    39541 gccatcagtg cgcaatcctt gcggctggtg cgctcaattt tgatgttgat cgccctgctt
    39601 tcggtcattg tgctgtggtc agaaatccat tccgctttcg gcttccttga aaatatttcg
    39661 ctgtgggatg tcacttccac ggtacagggc gtggaaagtc tggagccgat taccctcggt
    39721 gcggtgctga ttgccattct ggtatttatc atcaccacgc agctggtgcg caacctgccc
    39781 gcgctgctgg aactggcgat tttgcagcac ctggatttaa cgccgggtac gggctacgcc
    39841 atcaccacca tcaccaaata tctgctgatg ctgattggcg ggctggtcgg cttctcaatg
    39901 attggtattg agtggtcgaa attgcagtgg ttggttgccg cgctcggtgt tggtctcggt
    39961 tttggtttgc aggaaatttt cgccaacttt atctctggcc tgatcatcct gttcgaaaaa
    40021 ccgattcgca ttggcgatac ggtaacgatc cgcgatctta ctggtagcgt gacgaaaatt
    40081 aacacccgtg ccaccaccat cagcgactgg gaccgtaaag agataatcgt gccgaacaag
    40141 gcatttatta ccgagcagtt tatcaactgg tcgctctctg actcggtcac gcgcgtggtg
    40201 ttgacgattc cggcccctgc cgatgccaac agtgaagaag tgacggaaat cctgctcacc
    40261 gcagcgcgtc gctgctcgct ggtgatcgac aacccggcac cggaagtctt cctggtggat
    40321 ctgcaacagg ggattcagat tttcgagctg cgtatttacg ccgctgagat gggtcaccgt
    40381 atgccgctac gccatgagat ccaccagcta attctggcag gcttccacgc ccacggtatc
    40441 gatatgccat tcccgccctt ccagatgcgt ctggaaagcc tcaacggtaa acaaacgggg
    40501 agaacgctga cgtctgcggg caaaggtcgt caggcgggaa gtttgtaatg catgaagctg
    40561 ccaaatgatg tcaacgtggc tcatttagca gcttaacatc atcttcagtt atcaccggcc
    40621 ctggagagta tcatccatac gcattacttc ctgacaactc atcgtcggag agacgatgag
    40681 ttgcaggatt tcataggatc agtcgagagc taactcaggt ttattcgcca tgcgactgca
    40741 atagttctgg taaatcgcca tcgctaacag cgagaagaag acagggccgc caatcatcca
    40801 caatgtgctg tcccagtctc cggcttcaac cacaggctga ataatggtga agacgttcgc
    40861 aaatgtcacc accagaacga caaccaccgt cgcaatcatt gccgacatac gcgttttgaa
    40921 aatcacaaac ggtctgtcga gatcctgacg tgctttaaag aatgggaaag ccagcgcgag
    40981 gaacaagtaa ggaagcgtca tagacacgtt cgccatcagc gtcagcttgt taaagaacgc
    41041 cgatgcggta ccgccaccaa acgaaaccag caggatgaag acagtaacca gcccgcactg
    41101 catccacatg gcgatagacg gcatccccat cgcattcagg cgcgtcatcg gttccggcca
    41161 caatgctttc ggcgttccct ggatgatggc tttcagcggt gaatagcaaa gcgtaaagaa
    41221 cgcaccggta taagcgagga acatcgaaag tccggtaata cgcgcaaacc atacgcccag
    41281 cgacaatgac gcttctggtg acaaatgcag tgcgttaccc agcgtcaccc cgaggctctt
    41341 catcagcaca taggtaatat tgccgaggtt aacggaacca ttacttaata cctgctgcca
    41401 gtttgtgctg acgccccata aaaatattgc cagcgaataa ccgattgaaa taacaatagc
    41461 ggcaaaaaca atacctttgg caaagttctt ttctggattt tccgttttat cgaccagacc
    41521 accaaccgct tcaattccgc cataggcaaa aatagcaaat accacaaacg atagcattgc
    41581 cagaccggac tgataacctg ggttcggtga tgcaaggaaa ttaatatcct gcgcgaaatg
    41641 cccaccattt aataacaaaa tagtaatgct tactaacagc aatactaaat tcagacacat
    41701 tactgcaata ccgcccaccg cagtaatgcg ggcaatttta ttaatcccct ttgaagcaac
    41761 gacggtgacc agaatcatcc atgccactgc cagcagacca accacctgcg taggctccag
    41821 tccggcaata cgccagtgct gggtcatgtc gctaccatag aggaatgttg agaacggtac
    41881 ccaaactttc gcggaggtac tcaccatcca gatgatataa gaggaaaacc acataaatgt
    41941 gccaatgaag gcaaaacgtg gtccgacact attattcatc caggaataga taccgccttc
    42001 ttctttgcga taagcagctc ccatttcagc catcattaag gcgaatggaa taaagaataa
    42061 caatgcagaa aatatataaa agggaatcgc actataaccc attaagtaat aagccgatgg
    42121 gctattggca aatccaaata cggaagtaaa gatcatcaat atgagtccta tcagactcat
    42181 cttttttatc gtgtgaggca tgaaaccatc cttcatttca gcgcgtgtat aaaacgcgcc
    42241 cactgaactg cactgcccaa cattgagcgg gcagcgtaaa aaccaataca gaattgatac
    42301 ctggatgata tcaaatatcc agtcttaaat tgtggccttt agcggaaaaa tgaaaacata
    42361 tgctacagaa tggcgcttat cacgccattc ttcgcggtta attcagtaat ttttcagaat
    42421 tatgcccggt caacgctaaa ggcgatgact tcagccagtg tctccgcgcc cagcgccaac
    42481 atcaccagac gatcaacacc taatgccacg ccggaacagt caggcatacc gactttcaag
    42541 gcttcaatca gattctggtc aatggggtgc tgcggcaaac cgcgcgccgc gcgcttacgg
    42601 ttatcttgtt caaagcgttg ttgctgctca cgggcatccg tcaattcatg gaaaccattc
    42661 gccagctcaa tacctttata ataaacctca aagcgttcag cgacccgatg atcttcggta
    42721 ctgatttgcg ccagtgatgc ctggctggct ggaaagtggt acacaaaggt cggtttttct
    42781 ttgccaatat ttggctctac gccaaaggta aacagcaatt gtagcaacgt gtcgcggtct
    42841 tcttcggtat cagcaacgtt gcttaaatcc agtttcgctg ccacttcccg cagttgcgtt
    42901 ttgtcggcag agagcgggtc aatttccaga taacgcaaga aagcttgttg ataagaaagg
    42961 ctttctgctg ccgggcagtc cagcacttgt tgtaagagat cgtccacctc gttcatcaac
    43021 cggtacatat catagtgcgg tcgataccac tcaagcatag tgaactcagg gttgtgataa
    43081 cgccccatct cttcattacg gaagctgcgg cacagctgga ataccggccc acaaccggca
    43141 accagcaggc gcttcatatg gtattccggg ctggtcatta accagagatt catcccctgc
    43201 gaatgcccag ggccaacgaa acgtgtctca aacgggacca aatgaatatc ggttaccgtc
    43261 gcctggctca tacaaggcgt ctccacctcc agcactccac gatcggcaaa gaaacgacgg
    43321 atctccgcca taatcgccgc gcgttttaat aagttaggaa tggatgcgct cggctgccag
    43381 gatgccgttt cgctcatagt taaatctcca gtttttgaca agggcacgaa gtctactcgc
    43441 aacgcgacgg cgagacaaat tttacgcagg aatcaaacaa gggtgggcag tgactaaaaa
    43501 aagcacgatc tgatggttta gtaattaaat taatcatctt cagtgataat ttagccctct
    43561 tgcgcactaa aaaaatcgat ctcgtcaaat ttcagactta tccatcagac tatactgttg
    43621 tacctataaa ggagcagtgg aatagcgttc gcagaccgta actttcaggc acttaccctg
    43681 aagtacgggg ctgtgggata aaaacaatct ggaggaatgt cgtgcaaacc tttcaagccg
    43741 atcttgccat tgtaggcgcc ggtggcgcgg gattacgtgc tgcaattgct gccgcgcagg
    43801 caaatccaaa tgcaaaaatc gcactaatct caaaagtata cccgatgcgt agccataccg
    43861 ttgctgcaga agggggctcc gccgctgtcg cgcaggatca tgacagcttc gaatatcact
    43921 ttcacgatac agtagcgggt ggcgactggt tgtgtgagca ggatgtcgtg gattatttcg
    43981 tccaccactg cccaaccgaa atgacccaac tggaactgtg gggatgccca tggagccgtc
    44041 gcccggatgg tagcgtcaac gtacgtcgct tcggcggcat gaaaatcgag cgtacctggt
    44101 tcgccgccga taagaccggc ttccatatgc tgcacacgct gttccagacc tctctgcaat
    44161 tcccgcagat ccagcgtttt gacgaacatt tcgtgctgga tattctggtt gatgatggtc
    44221 atgttcgcgg cctggtagca atgaacatga tggaaggcac gctggtgcag atccgtgcta
    44281 acgcggtcgt tatggctacc ggcggtgcgg gtcgcgttta tcgttacaac accaacggcg
    44341 gcatcgttac cggtgacggt atgggtatgg cgctaagcca cggcgttccg ctgcgtgaca
    44401 tggaattcgt tcagtatcac ccaaccggtc tgccaggttc cggtatcctg atgacagaag
    44461 gctgccgcgg tgaaggtggt attctggtca acaaaaatgg ctaccgttat ctgcaagatt
    44521 acggcatggg cccggaaact ccgctgggcg agccgaaaaa caaatatatg gaactgggtc
    44581 cacgcgacaa agtttctcag gccttctggc acgaatggcg taaaggcaac accatctcca
    44641 cgccgcgtgg cgatgtggtt tatctcgacc tgcgtcacct cggcgagaaa aaactgcatg
    44701 aacgtctgcc gttcatctgc gaactggcga aagcgtacgt tggcgtcgat ccggttaaag
    44761 aaccgattcc ggtacgtccg accgcacact acaccatggg cggtatcgaa accgatcaga
    44821 actgtgaaac ccgcattaaa ggtctgttcg ccgtgggtga atgttcctct gttggtctgc
    44881 acggtgcaaa ccgtctgggc tccaactccc tggcggaact ggtggtcttc ggccgtctgg
    44941 ccggtgaaca agcgacagag cgtgcagcaa ctgccggtaa tggcaacgaa gcggcaattg
    45001 aagcgcaggc agctggcgtt gaacaacgtc tgaaagatct ggttaaccag gatggcggcg
    45061 aaaactgggc gaagatccgc gacgaaatgg gcctggctat ggaagaaggt tgcggtatct
    45121 accgtacgcc ggaactgatg cagaaaacca tcgacaagct ggctgagctg caggaacgct
    45181 tcaagcgcgt gcgcatcacc gacacctcca gcgtgttcaa caccgacctg ctctacacca
    45241 ttgaactggg tcacggtctg aacgttgctg aatgtatggc gcactccgca atggcacgta
    45301 aagagtcccg cggcgcgcac cagcgtctgg acgaaggttg caccgagcgt gacgacgtca
    45361 acttcctcaa acacaccctc gccttccgcg atgctgatgg cacaactcgc ctggagtaca
    45421 gcgacgtgaa gattactacg ctgccgccag ctaaacgcgt ctacggtggc gaagcggatg
    45481 cagccgataa ggcggaagca gccaataaga aggagaaggc gaatggctga gatgaaaaac
    45541 ctgaaaattg aggtggtgcg ctataacccg gaagtcgata ccgcaccgca tagcgcattc
    45601 tatgaagtgc cttatgacgc aactacctca ttactggatg cgctgggcta catcaaagac
    45661 aacctagcac cggacctgag ctaccgctgg tcctgccgta tggcgatttg tggttcctgc
    45721 ggcatgatgg ttaacaacgt gccaaaactg gcatgtaaaa ccttcctgcg tgattacacc
    45781 gacggtatga aggttgaagc gttagctaac ttcccgattg aacgcgatct ggtggtcgat
    45841 atgacccatt tcatcgaaag tctggaagcg atcaaaccgt acatcatcgg caactcccgc
    45901 accgcggatc agggtactaa catccagacc ccggcgcaga tggccaagta tcaccagttc
    45961 tccggttgca tcaactgtgg tctgtgctac gccgcgtgcc cgcagtttgg cctgaaccca
    46021 gagttcatcg gtccggctgc cattacgctg gcgcatcgtt acaacgaaga tagccgcgac
    46081 cacggtaaga aggagcgtat ggcgcagttg aacagccaga acggcgtatg gagctgtact
    46141 ttcgtgggct actgctccga agtctgcccg aaacacgtcg atccggctgc ggccattcag
    46201 cagggcaaag tagaaagttc gaaagacttt cttatcgcga ccctgaaacc acgctaagga
    46261 gtgcaacatg acgactaaac gtaaaccgta tgtacggcca atgacgtcca cctggtggaa
    46321 aaaattgccg ttttatcgct tttacatgct gcgcgaaggc acggcggttc cggctgtgtg
    46381 gttcagcatt gaactgattt tcgggctgtt tgccctgaaa aatggcccgg aaagctgggc
    46441 gggattcgtc gactttttac aaaacccggt tattgtgatc attaacctga tcactctggc
    46501 tgcagctctg ctgcacacca aaacctggtt tgaactggca ccgaaagcgg ccaatatcat
    46561 tgtaaaagac gaaaaaatgg gaccagagcc aattatcaaa agtctctggg cggtaactgt
    46621 ggttgccacc atcgtaatcc tgtttgttgc cctgtactgg taaggagcct gagatgatta
    46681 atccaaatcc aaagcgttct gacgaaccgg tattctgggg cctcttcggg gccggtggta
    46741 tgtggagcgc aattattgcg ccggtgatga tcctgctggt gggtattctg ctgccgctgg
    46801 ggctgtttcc gggtgatgca ctgagctacg agcgcgttct ggcgttcgcg cagagcttca
    46861 ttggtcgcgt attcctgttc ctgatgatcg ttctgccgct gtggtgtggt ttacaccgta
    46921 tgcaccacgc gatgcacgat ctgaaaatcc acgtacctgc aggcaaatgg gttttctacg
    46981 gtctggctgc tatcctgaca gttgtcacgc tgattggtgt cgttacaatc taacgcatcg
    47041 ccaatgtaaa tccggcctgc cgttggcagg ccgttttgta tggaaaccag accctatgtt
    47101 caaaatgacg ctctgcgcct tattaattac cgcctcttgc tccacatttg ctgcccctca
    47161 acaaatcaac gatattgtgc atcgcacaat taccccgctt atagagcaac aaaagatccc
    47221 cggtatggcg gtggcggtaa tttatcaggg taaaccttat tactttacct ggggctatgc
    47281 ggacatcgcc aaaaagcagc ccgtcacaca gcaaacgttg tttgagttag gttcggtcag
    47341 caaaacattt acgggcgtac ttggtggcga cgctattgct cgaggggaaa tcaagttaat
    47401 cgatcccgcg acaaaatact ggcctgaact taccgctaaa cagtggaatg ggatcacact
    47461 attacatctt gcgacctaca ccgctggcgg cctgccattg caggtgccgg atgacgttaa
    47521 atcctcaagc gacttgctgc gcttctatca aaactggcag cctgcatggg ctccaggaac
    47581 acaacgtctg tatgccaact ccagtatcgg tttgttcggc gcactggctg tgaagccgtc
    47641 tggtttgagt tttgagcagg cgatgcaaac tcgtgtcttc cacccactca aactcactca
    47701 tacgtggatt aatgtgccgt ccgcagaaga aaagaattac gcctggggat atcgcgaagg
    47761 taaggcagtg catgtttcgc caggggcgtt agatgctgaa gcttatggtg tgaagtcgac
    47821 cattgaagat atggcccgct gggtgcaaag caatttaaaa ccccttgata tcactgagaa
    47881 aacgcttcaa caagggatac aactggcaca atctcgctac tggcaaaccg gcgatatgta
    47941 tcagggcctg ggctgggaaa tgctggactg gccggtaaat cctgacatca tcattaacgg
    48001 cagtgacaat aaaattgcac tggcagcacg ccccgtaaaa gcgattacgc ccccaactcc
    48061 tgcagtacgc gcatcatggg tacataaaac aggggcgacc ggcggatttg gtagctatgt
    48121 cgcgtttatt ccagaaaaag agctgggtat cgtgatgctg gctaacaaaa actatcccaa
    48181 tccagcgaga gtcgccgccg cctggcagat tctcaacgct ctacagtaaa atttcatcgg
    48241 gtccgaattt tcggaccttt tctgcgcttt tccttgctgt catctacact tagaaaaaac
    48301 cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag ctgcatttct
    48361 ggtcgttgcc tgcagttctc ctacgccgcc ccgtggcgtg accgtagtga ataatttcga
    48421 tgctaaacgc tatctcggta cctggtatga gattgcccgt tttgatcacc gctttgaacg
    48481 tggactggaa aaagtcaccg caacatacag cctgcgggat gacggcggcc tgaatgtcat
    48541 taataaaggc tataaccctg acagagaaat gtggcagcag agtgaaggga aagcgtactt
    48601 taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt tctatggcgg
    48661 ttataacgtt attgcactcg atcgggaata ccgccatgcg ctggtttgcg gcccggaccg
    48721 cgactacctg tggatactct cccgcacacc aaccatttct gacgaagtga aacaggagat
    48781 gctggcagtc gcgacccagg aagggtttga tgtcagtaag tttatttggg tacagcagcc
    48841 cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta acgccagact
    48901 cgccaggcgc atcgggttag cggactcgcc gagcagcaca atgccggtga tggccgcgcc
    48961 gactgcgcca atacccgtcc acacggcata agccgtccct actggtaacg atttcatcgc
    49021 ccaggcaagt agcgccatac tgacaatcat cgccgtcacg gtaataacac ttggcgtcaa
    49081 acgactaaag ccgtgggtat atttcaggcc aacggcccat accacttcca gcagaccagc
    49141 aataactaag ataatccagg acatatcagg ctccggagaa ttggggccgt ccccggtgaa
    49201 aagaggcgtt tgcaggtcgt cctgcaaagc tgatggttga acggatattt tgccaggtgg
    49261 caggggaatt tcaactatta cctttgtgac tatcgca
//
LOCUS       DAPOZO010000029        48454 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPOZO010000029 DAPOZO010000000
VERSION     DAPOZO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.029"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            26..343
                     /locus_tag="ROI21_001812"
     CDS             26..343
                     /locus_tag="ROI21_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HDX5110737.1"
                     /translation="MQPVFFIKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEGIDKET
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYECGRHQQRVEAITAIEQ
                     RRN"
     gene            412..741
                     /gene="yjdP"
                     /locus_tag="ROI21_001813"
     CDS             412..741
                     /gene="yjdP"
                     /locus_tag="ROI21_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDX5110738.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSHDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(776..1534)
                     /gene="phnP"
                     /locus_tag="ROI21_001814"
     CDS             complement(776..1534)
                     /gene="phnP"
                     /locus_tag="ROI21_001814"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDX5110739.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACVRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDSIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTMEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNEVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(1536..1970)
                     /gene="phnO"
                     /locus_tag="ROI21_001815"
     CDS             complement(1536..1970)
                     /gene="phnO"
                     /locus_tag="ROI21_001815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDX5110740.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNIGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(1957..2523)
                     /gene="phnN"
                     /locus_tag="ROI21_001816"
     CDS             complement(1957..2523)
                     /gene="phnN"
                     /locus_tag="ROI21_001816"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDX5110741.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSAGSENHIALSEQEFFTRAGQNLLALSWHANGLYYGIGVEIDLWLHAGFDVLANGSR
                     AHLPQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHT
                     LNNDGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(2523..3659)
                     /gene="phnM"
                     /locus_tag="ROI21_001817"
     CDS             complement(2523..3659)
                     /gene="phnM"
                     /locus_tag="ROI21_001817"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDX5110742.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSRLPDAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(3656..4336)
                     /gene="phnL"
                     /locus_tag="ROI21_001818"
     CDS             complement(3656..4336)
                     /gene="phnL"
                     /locus_tag="ROI21_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDX5110743.1"
                     /translation="MINVQNVSKTFILHQQNGVRLHVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLITAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(4447..5205)
                     /gene="phnK"
                     /locus_tag="ROI21_001819"
     CDS             complement(4447..5205)
                     /gene="phnK"
                     /locus_tag="ROI21_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDX5110744.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPSNRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5202..6047)
                     /gene="phnJ"
                     /locus_tag="ROI21_001820"
     CDS             complement(5202..6047)
                     /gene="phnJ"
                     /locus_tag="ROI21_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDX5110745.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(6040..7104)
                     /gene="phnI"
                     /locus_tag="ROI21_001821"
     CDS             complement(6040..7104)
                     /gene="phnI"
                     /locus_tag="ROI21_001821"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDX5110746.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7104..7688)
                     /gene="phnH"
                     /locus_tag="ROI21_001822"
     CDS             complement(7104..7688)
                     /gene="phnH"
                     /locus_tag="ROI21_001822"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDX5110747.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(7685..8137)
                     /gene="phnG"
                     /locus_tag="ROI21_001823"
     CDS             complement(7685..8137)
                     /gene="phnG"
                     /locus_tag="ROI21_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDX5110748.1"
                     /translation="MHADTATRQHWMSILAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRATVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8138..8863)
                     /gene="phnF"
                     /locus_tag="ROI21_001824"
     CDS             complement(8138..8863)
                     /gene="phnF"
                     /locus_tag="ROI21_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDX5110749.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGIAEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(8884..9663)
                     /gene="phnE"
                     /locus_tag="ROI21_001825"
     CDS             complement(8884..9663)
                     /gene="phnE"
                     /locus_tag="ROI21_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDX5110750.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(9789..10805)
                     /gene="phnD"
                     /locus_tag="ROI21_001826"
     CDS             complement(9789..10805)
                     /gene="phnD"
                     /locus_tag="ROI21_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX5110751.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(10830..11618)
                     /gene="phnC"
                     /locus_tag="ROI21_001827"
     CDS             complement(10830..11618)
                     /gene="phnC"
                     /locus_tag="ROI21_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDX5110752.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            complement(11751..12194)
                     /gene="yjdN"
                     /locus_tag="ROI21_001828"
     CDS             complement(11751..12194)
                     /gene="yjdN"
                     /locus_tag="ROI21_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDX5110753.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(12354..12689)
                     /locus_tag="ROI21_001829"
     CDS             complement(12354..12689)
                     /locus_tag="ROI21_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDX5110754.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            13091..15319
                     /gene="crfC"
                     /locus_tag="ROI21_001830"
     CDS             13091..15319
                     /gene="crfC"
                     /locus_tag="ROI21_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDX5110755.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDVPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPALIRHMPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAKPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANYFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQAVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            15316..16194
                     /locus_tag="ROI21_001831"
     CDS             15316..16194
                     /locus_tag="ROI21_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDX5110756.1"
                     /translation="MIKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            16458..17960
                     /gene="proP"
                     /locus_tag="ROI21_001832"
     CDS             16458..17960
                     /gene="proP"
                     /locus_tag="ROI21_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDX5110757.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            18072..18161
                     /gene="pmrR"
                     /locus_tag="ROI21_001833"
     CDS             18072..18161
                     /gene="pmrR"
                     /locus_tag="ROI21_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDX5110758.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(18137..19237)
                     /gene="pmrB"
                     /locus_tag="ROI21_001834"
     CDS             complement(18137..19237)
                     /gene="pmrB"
                     /locus_tag="ROI21_001834"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDX5110759.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(19238..19906)
                     /gene="basR"
                     /locus_tag="ROI21_001835"
     CDS             complement(19238..19906)
                     /gene="basR"
                     /locus_tag="ROI21_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDX5110760.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(19903..21546)
                     /gene="eptA"
                     /locus_tag="ROI21_001836"
     CDS             complement(19903..21546)
                     /gene="eptA"
                     /locus_tag="ROI21_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDX5110761.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPTTSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(21650..22987)
                     /gene="adiC"
                     /locus_tag="ROI21_001837"
     CDS             complement(21650..22987)
                     /gene="adiC"
                     /locus_tag="ROI21_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDX5110762.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(23124..23885)
                     /gene="adiY"
                     /locus_tag="ROI21_001838"
     CDS             complement(23124..23885)
                     /gene="adiY"
                     /locus_tag="ROI21_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDX5110763.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(24209..26476)
                     /gene="adiA"
                     /locus_tag="ROI21_001839"
     CDS             complement(24209..26476)
                     /gene="adiA"
                     /locus_tag="ROI21_001839"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDX5110764.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGITRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(26675..27583)
                     /gene="melR"
                     /locus_tag="ROI21_001840"
     CDS             complement(26675..27583)
                     /gene="melR"
                     /locus_tag="ROI21_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDX5110765.1"
                     /translation="MNKDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVSINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            27866..29221
                     /gene="melA"
                     /locus_tag="ROI21_001841"
     CDS             27866..29221
                     /gene="melA"
                     /locus_tag="ROI21_001841"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDX5110766.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            29324..30745
                     /gene="melB"
                     /locus_tag="ROI21_001842"
     CDS             29324..30745
                     /gene="melB"
                     /locus_tag="ROI21_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDX5110767.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(30884..31513)
                     /locus_tag="ROI21_001843"
     CDS             complement(30884..31513)
                     /locus_tag="ROI21_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDX5110768.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGHKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(31636..33282)
                     /gene="fumB"
                     /locus_tag="ROI21_001844"
     CDS             complement(31636..33282)
                     /gene="fumB"
                     /locus_tag="ROI21_001844"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDX5110769.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDSGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(33360..34700)
                     /gene="dcuB"
                     /locus_tag="ROI21_001845"
     CDS             complement(33360..34700)
                     /gene="dcuB"
                     /locus_tag="ROI21_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDX5110770.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(35271..35990)
                     /gene="dcuR"
                     /locus_tag="ROI21_001846"
     CDS             complement(35271..35990)
                     /gene="dcuR"
                     /locus_tag="ROI21_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDX5110771.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(35987..37618)
                     /gene="dcuS"
                     /locus_tag="ROI21_001847"
     CDS             complement(35987..37618)
                     /gene="dcuS"
                     /locus_tag="ROI21_001847"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDX5110772.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            37799..38029
                     /locus_tag="ROI21_001848"
     CDS             37799..38029
                     /locus_tag="ROI21_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDX5110773.1"
                     /translation="MDRALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            38041..38313
                     /locus_tag="ROI21_001849"
     CDS             38041..38313
                     /locus_tag="ROI21_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDX5110774.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            38541..38837
                     /gene="ghoS"
                     /locus_tag="ROI21_001850"
     CDS             38541..38837
                     /gene="ghoS"
                     /locus_tag="ROI21_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDX5110775.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            38865..39038
                     /gene="ghoT"
                     /locus_tag="ROI21_001851"
     CDS             38865..39038
                     /gene="ghoT"
                     /locus_tag="ROI21_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDX5110776.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(39157..40674)
                     /gene="lysS"
                     /locus_tag="ROI21_001852"
     CDS             complement(39157..40674)
                     /gene="lysS"
                     /locus_tag="ROI21_001852"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDX5110777.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(40911..42368)
                     /gene="dtpC"
                     /locus_tag="ROI21_001853"
     CDS             complement(40911..42368)
                     /gene="dtpC"
                     /locus_tag="ROI21_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDX5110778.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMVSGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(42427..44574)
                     /gene="cadA"
                     /locus_tag="ROI21_001854"
     CDS             complement(42427..44574)
                     /gene="cadA"
                     /locus_tag="ROI21_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDX5110779.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(44654..45988)
                     /gene="cadB"
                     /locus_tag="ROI21_001855"
     CDS             complement(44654..45988)
                     /gene="cadB"
                     /locus_tag="ROI21_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDX5110780.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(46353..47891)
                     /gene="cadC"
                     /locus_tag="ROI21_001856"
     CDS             complement(46353..47891)
                     /gene="cadC"
                     /locus_tag="ROI21_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDX5110781.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPDTDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT        11562 a        12094 c        12794 g        12004 t
ORIGIN      
        1 ggcaaatact gagcacaatt aatcaatgca accggtgttt tttattaaaa aacgtaccgg
       61 agcaatgggc ggtcatgtcg ctggcaccgc aggattctgc gttgctgtgc cagcctttta
      121 ttcatggtca atgagtgagg ggattgataa agaaaccagg tcgtcagagc gcactgatta
      181 tccgcattac gccagtgaag ttgcactggc ttttggttca cgagtggttg gcctcgaact
      241 ggcaaaaatg attgtggatg cgtggctggg cgcacagtac gaatgcggtc gtcatcaaca
      301 acgtgtggag gcgattacgg caatagagca gcggagaaat tgagattcat ccactacttg
      361 catggatgag taatgactaa tgtggataga gtttcttttt gaggttggct aatgaaacgc
      421 tttccgctat ttcttttatt caccctgctc acgctgtcca ccgttccggc ccaggccgat
      481 attatcgacg acaccattgg caatattcag caggcgatta acgatgctta taaccctgac
      541 cgtgggcgag actatgaaga ttcgcacgac gacggctggc agcgtgaagt gagcgacgat
      601 cggcgcagac aatatgacga ccgccgccgc cagtttgaag atcgccgcag gcagctggac
      661 gatcgccagc gccagcttga tcaggagcgt cggcagctag aagatgaaga gcggagaatg
      721 gaagatgagt atgggcgttg atgttgagtt tagagcaggc atcgcccctc acccatcacg
      781 ccaccccaat ctccatccca tcaaaccccg cctcaaaccc cgacggcagt gcgttttcca
      841 tcagccacgc atcaaactgg tggctgatat gggtcagaat cacccgcggc gagcggataa
      901 cctcattcag tgccagcacg gtgtttaaat cgcagtgatt acgcggtgca tcctcgcgcg
      961 gtgggtggct gcaatcaatc accattacct gtggctgatt attgcgtaaa aatttcagcg
     1021 ttttttccgg caggcctgcc gtgtcagaca gccacgccac ccggctatgt gccgtttcca
     1081 gtagatagcc gaaagtgagt ttcgaatggt tgagcggcag cggcgtgacc tgtaacccct
     1141 gtaaatcaaa caccacaaac ggttccatcg tgtggctgaa atcaagcagg cccggatgtt
     1201 taaacagatc gtcgcagccc tgttcatccg gcgggccgta gaccgggatc gaatcgccca
     1261 cgccccagcg cagcggaaac agcccctgca cgtgatccat atggtagtgc gtcagcaaaa
     1321 actgctggaa cgatccgggc gaccagcgat cggcaagatc gtgcagcccg gcgtcgatca
     1381 gggtgatcgc gtcgttaaac ttcacgacgc cgctgcatgg ctggcggcga tactgcggtg
     1441 agcgccgcgc tctgacgcag gccacgcact cacagcccca tgcgggaacg ccctgtgcgc
     1501 cgccagtgcc agtaagtgtg agggtcaggc tcatgttaca gcgccttggt gaagcggaag
     1561 tggctctgct catagccttc gcgcagatag aaacggtgcg cgtcgtggcg cttgacgttg
     1621 gtggaaagct cggtcatttc tgccccggcc tggcgggctt cttcttctgc ccatgccagt
     1681 aatttactgc cgatgttcag accgcgcgcc tgcggcatca ccaccagttc ctgaatttcg
     1741 ccgatccagt tgacatgatg cagatgaaac tgcaaatgca ggccgatcat gccgacaact
     1801 tcgccatcca gcagcgccag atggtagcgc atgttcggat cgcgcagatt ggcgttaaac
     1861 cccacgcgaa acgcatggtg gtcaaactcc gcctgtttta gctcacaaat cagcgcgtaa
     1921 actgcgtcgg tatcgtactg cgtggccggg cgaagctcac aagcaggcat ggtgtttctc
     1981 cttctgatga atcagcgtca gcagcgtgtc gaccgactgg cgcaggctgc cgtcattatt
     2041 gagcgtatgg caatcctgag gagtatagcg ggcggcgcgc gccagccggg cgttaatttc
     2101 actggcgttt tcacggccac ggttttccag gcgctggcgc aggatctccg gcgaaacctg
     2161 taagcaaatg ggcagcagcg ccgattgata gcgcgcccgc gcctgcggca aatgcgcgcg
     2221 tgagccgttg gccagcacgt caaatccagc gtgcagccag agatcaatct cgacgccgat
     2281 gccataatac aggccgttgg cgtgccagct taaggccaga aggttctgcc ccgcgcgggt
     2341 aaaaaactcc tgctcgctca gggcgatatg gttttcactt ccggcacttc cggcgctggc
     2401 ggcgcgcgtg atgtagcgat gcgctaccag tagctgagtt tgttcccgct ggcggagttc
     2461 cgtcaacaga ctgtcttttc cggagccaga ggcccccatt aaccaaatca gttttcccgt
     2521 catcagaaca cccttttacc ctgacgccag acgtggtcga tatgaatgtg gttgcccttg
     2581 cgatgcgcca gcaccaggtc ggcgcgtttg ccctcgccaa tcactccgcg atcctgaaga
     2641 ttaagcgcct gcgccggatt tttagtcacc agcttcaccg cctgcggcag cgtaaagcgg
     2701 ttgctctcgt catcggcgac gcgaaatgcc gcatcgagca ggctggcggg gtagtagtcg
     2761 gaagagagga tatccagcag gccaagctgc gccagttcac tggccgccac gttgccggag
     2821 tgcgaaccgc cgcgcacgat attcggcgcg cccatcagca cgttcatgcc atgcttgcgc
     2881 gaggcttctg ccgcttcgaa cgtggtggga aattcggcga tcacgctgcc aagctggtga
     2941 gattcggcaa cgtgggcgtg cgtggcgtca tcgtggctgg cgagggcaat gtgtcgcgcg
     3001 cggcacattg cggcgatggt ttcgcgattt ggctgcgacc agcgtgcggc gagcgccaat
     3061 tgctcttctt cgtactgctg catctgcgca tcggtgaggg aatatttgcc ctgataatat
     3121 tcgcgatact tctcgcgatt ggcgaactgg cgctggcctg gcgagtggtc catcagcgat
     3181 accagcgtca ccggttcgcg ctgcaccagt ttttcaaaca gcggcagcgt ggtgtggtgc
     3241 ggcagttcgc agcgcaggtg caggcggtgc tcggcgcggt tgacgccgcg tttctgcgtc
     3301 tcttcgatgg cgttgatcat cttctccaga ttctccagcc gatcgccgcc gtcgcgcaca
     3361 tcaccaatcg ccacggcgtc cagtacggtg gtgatgccgc ttgccaccat cagcgcgtcg
     3421 tggctgctca tcgccgaatg ggcgggccag tcgactttcg ggcgcggggt gaagaattta
     3481 tcgaggttgt cggtatgcag ctcaatcagt ccgggcagta gccagccacc ttcgccgtcc
     3541 atcgcgtccg gcaggcggct ctggctttcg gcaaaggcgc ggatttcacc gtcctgcatc
     3601 tccagcgaac cgtgcaccac ttcgttttcc agcaccagct taacgttatt gataatcatg
     3661 aagaggctcc cattgggtgc aggcggtcgg cgacgtcatt gcgtacggct tcgtcatgga
     3721 agataccgac gatggccgcg ccgcgggctt tggcttcgcg gatcagttcc actacggcgg
     3781 cgctgttttt agcgtccagc gaggcggtag gttcgtcgag cagcagaatg ggatagtcga
     3841 cgataaagcc acgggcgatg ttgacgcgct gctgttcgcc gccggagaag gtcgatggtg
     3901 caaggtgcca caggcgttcc ggcacgttca ggcgtgttag cagacgcgcg gctttagcgg
     3961 cgcaggcttc acgcggtacg ccggtatcga gcagaggctg catcaccact tccagtgccg
     4021 aaatacgcgg gatgacgcgc agaaactggc tcacccagcc gatggtggtt ttgcggatct
     4081 ccaccacttt gcgggctggc gcggtgatca ggtctaccca ctcatcaccg tgtttgatct
     4141 ggatttgacc ttcatcgggc agatagttgg cgtacagcga gcgtagcagg gttgattttc
     4201 cactgccgga gtggccgtgg agtaccacgc attcgcctgc gttgacggtg agcgaggcac
     4261 gttgtaagac gtgcaggcgc acgccgtttt gctggtgcag gatgaaggtt ttactgacgt
     4321 tttgtacgtt aatcattttt cgcctcgtgg ttcgggtgtc ggatgcgacg ctgacgcgtc
     4381 ttatccgacc tacggggagc gcatttgtcg gccggataag gcgtttacgc cgtatccggc
     4441 actggctcaa ttctgcaaaa ccgatgacac cagcagctgg gtatacggat gatgcgggtc
     4501 gtcgagcacg cggtcggtta acccactctc caccacttgc ccctgtttca tcaccagcaa
     4561 acggtccgcc agcaggcggg cgacgcccag atcgtgggtg acaatcacca ccgccagatt
     4621 cagctccacc accagaccgc gcagcagatc gagcaggcgc gcctgtaccg atacatccag
     4681 cccgccggtt ggctcgtcca taaacaccag cttcggatgc gtcaccaggt tgcgggcaat
     4741 ctgcaaacgt tgctgcatac cgccggaaaa ggtggtcggc aggtcgtcga tccggttgga
     4801 gggaatctcc acctcctcca gccacttctg cgcggtggcg cgaatatcgc cgtaatgacg
     4861 cgccccggtc gccatcagcc gctcgccgat attgccgcct gccgacacct ggcggcgcag
     4921 gccgtcgagc ggatgctgat gcaccacgcc ccattcggtg cgcagcaggc gacggcggtc
     4981 ggcctcgctc atgccataga gggagcggtt ttcatagcga atctccccct gctgcggcgt
     5041 caggcgcgcg gagattgatt tcagcagcgt ggtcttcccg gagccggatt ccccgacgat
     5101 gcccagcact tcccccggcc ataaatcaaa agagacatcg ctaaagcctt tgcccggcgc
     5161 gtaaaggtgg gtcaggttat tgaccgaaag taacggttga ttcattggct tttcgcctcg
     5221 ctctgttggc ggcaataatc ggtatcggag cagacaaaca tgcggtttcc ggcgtcatcc
     5281 agtaccactt cgtcgagata gctgtgggtc gatccgcaga tggcgcacgg ctcatcccac
     5341 tgttgaacgg tgaacgggtg atcgtcgaaa tcgaggcttt ccacgcgggt aaacggcggc
     5401 accgcataga tgcgcttctc gcgtcctgcg ccgaacagct gcagggcggg catcatgtcc
     5461 atttttgggt tatcgaattt cgggatcggc gacgggtcca tcacgtagcg cccgttcacc
     5521 ttcaccggat aggcataggt ggtggcgata tgaccgaagc gggcgatatc ttcatacagc
     5581 ttcacctgca tcacgccgta ctcctccagc gcgtgcatag tgcgggtctc cgtttcgcgc
     5641 ggctcgataa agcgcagcgg ttcggggatc ggcacctgga aaataatgat ctgatcttcg
     5701 gtcagcggcg tttcggggat gcggtggcgc gtctggataa gcgtcgcatc gtccgtacgt
     5761 tcagtggtgt ttaccccggt cacgcgctta aagaagttgc gaattgatac ggcgttggtg
     5821 gtgtcatccg caccctggtc aatcaccttc aacacgtcgc tttcgccaat cacgctggcg
     5881 gtaagctgga taccaccagt tccccagccg tacggcatcg gcatctcgcg cccgccaaac
     5941 ggcacctgat aaccggggat cgccaccgct tttaagatgg cgcggcggat catgcgcttg
     6001 gtctgctcgt cgaggtaggc aaagttgtag ccgctcagat tagccatggt tctgctcctg
     6061 ttgcagacgt ttgagtagct ccagttcggc ctggaaatcg acgtagtgcg ggagtttaag
     6121 gtgcgagaca aagcctgcgg cttcgacgtt gtcggcatgc gccagcacga actcttcatc
     6181 ctgcgccggg cctgtcgcgt gctcgccgta ctccggggct tgtagagcgc ggtcaaccag
     6241 cgccatcgcc atcgctttgc gctcgctcat gccgaacacc agcccgtagc cgcgcgtgaa
     6301 gtgcggcggc tcatccggcg ggtcgataaa accgttgacc atttcacatt cggtcatcag
     6361 cagttcgccg acgttcaccg caaatcccag ctcttccggc acaatagaca cgtcgatata
     6421 gccactgcgg atctcgcccg cgaacgggtg attgcgcccg taaccacgct gggtggagta
     6481 ggccagcgcc agcaaatagc cttcgtcgcc gcgcattaat tgttgcaggc gggaggagcg
     6541 tgagcagggg taaaccggcg gcgtgcgggt gatgtcgtcc ggctgtgcgc cgctatcttc
     6601 ttcaaacttc gccagcccct gacgcgccag caggctgaaa acgtgcggcg acggctgttg
     6661 ttcgctgtcg gctgtggtca gcgtcggcgc ttcgccgttt gccagcaggg tgaaatcgag
     6721 caggcgatgg gtgtagtcgt aggttgggcc aagcagctgg ccgccgggaa tatctttata
     6781 aaccgctgaa atacgccgtt cgagacgcat cccggtggtg tcgagcggct cgcttaccgc
     6841 cagcttcgcc agcgtggtgc ggtaggcgcg tagcaggaaa atcgcttcga cgttatcgcc
     6901 gctggcctgt ttcagcgcca gcgccgccag ttcgcggtcg gcaatgccgc cttcggtcat
     6961 cacgcgatct actgcaagat taagctgctg ttcaatctgg gcgacgctca gttcaggcaa
     7021 atcggtattg ccccggcgtc ggctctcttg cagggcgtgg gcggcgtcga tcgccttttc
     7081 gccccctttc acggcaacgt acatcagcac acctccacat gagtggttcg cgggatagcc
     7141 agcaggcgct cgccgcaggt caggatcagg tcgatgccga gcgggaacgg gtgcgggcgc
     7201 tcggtaagtt cgtgcagaat gcactccggc agctgcggag cgatcattcg ttcttcggca
     7261 atacccgcgc cagtaaggcg cagcatgcgc ccgccgctca ggctgacgac ctgtaaaatc
     7321 agtgtcgcgc ccgcttctgg cgcaacagcg gtgccgctgg aaagggcgtt aagctgttcg
     7381 ctggaaatcg cctcatccgt taccgcgaag gtcgccagtt ccggctgatt gaccagcggc
     7441 gcgttggtat gaaaacgcag gctctggctg acgatatcgt tacttaatgg cgcagaaagc
     7501 cacaccggcg tgtcgttatc ggccagcgtc agcagcacgc tggtggtggc gatattcagc
     7561 ggctgccagc cgcgtttgag ctgatgcagg gcgacaatca cgcccggctc gctcatggcc
     7621 tttaacaggc gacgaaaact gtgctgggca tcctgcacag gaagcataaa agcggtttcc
     7681 agggtcatgc gttatctccg cgaaccatcg taaagaagtc gacccggctg gcgttcactt
     7741 cggcctggcg tgcggcaatg cgtgccatac ggtcagcgtc cagcggggca ataagggttt
     7801 ctgataagtt ttgaaaatgg cgggtttgct gcatcagcgc gtcaatcagc gcgcagcgtt
     7861 cggcgtgctg tttgtcgcgc cccagcaccc agctgtagcc gagcgtgccg tcagtcaggc
     7921 gtacggtggc gcgggtgagc gtggcgtcgc cagcaaaaaa gcgttcgccg gtgccaccca
     7981 tccgcgcctg aatctgcacc aggccggttt cagcggcgcg gattacttca tagtcggcag
     8041 tgatgttcag cgccttcagg cgggctgcca gttcagccgg ttggctgtgt gccagtatgg
     8101 acatccagtg ctggcgggtc gcggtatctg cgtgcattca gtgctccata gtgaattcaa
     8161 tcatgtcggc gcgcgtcagg ctgacggagt actccgccgg gctgctttca ccgtcacggt
     8221 ggttaagggt gcgcacgcac agcagcggcg acatattcgg gatttcaaga cgctggcact
     8281 ctttggcctg ggcgcggcgg gcgctgatcc gcgtctggct gcggcgcagc gcaatccccg
     8341 tttgctcacg cagaaaatcg tgcagcgagc cgctgtcgaa gcgttgcagc gtcggccaga
     8401 gggtgaggtc cgcaaagtag tggtcgatta aacagagcgc gacgccgttg acccggcgca
     8461 gggtgcgcag gtggataacg ttctcgccct cggcgatccc cagcgcgtca gcgacgtgac
     8521 cggaggccgg gcgcagcaca gacagcagtt tttcgctggt gggatggctg ccctgatcca
     8581 gcaggttctg gctaaaacgc gcctgggcgt tgagcgggta atcgaacggg cgcatcagca
     8641 ccagcacgcc gacgccctga cggcgctgta cccagccttt ttccaccagt tggtcgatgg
     8701 cgcggcgcag ggtgtggcgg ttcacctcaa agcgcgccgc cagttgctgc tcggcgggaa
     8761 gatagtcgcc gcagcggtag tgttgacgaa gctcctgctc aagttttgcg gctatctcct
     8821 gatagcgtgt tgggtagctg gtcggatgtg tagacaagtg catagatatc aatgcctcgc
     8881 ttatcagata aagtgcttac gcaaccgttg agagaggaaa tccagcaggc tgaccgtgac
     8941 gataatcagc accatcaggg cgcaggtttg ctggaactgg aaaccgcgaa tagcttccca
     9001 cagggtgacg ccgatcccgc ccgcgccgac catgccaaca acggtggccg aacggacgtt
     9061 ggattcgaag cgatagaggg agtaggagat cagcagcggc atcacctgcg gcagcacgcc
     9121 gtagaggatc tcttcgagct tgttggcacc ggtggcgcga ataccttcca ccgggccggg
     9181 ttcaattgct tctaccgctt cggaaagcag tttggagagc acgccggtgg tgtggataaa
     9241 cagcgccagt acgccagcga acggaccgag accgacggcg accacgaaca gcatggcgaa
     9301 gaccatctcg ttaatggcgc ggcaggcgtc catcaggcgg cgtacgggct ggtaaaccca
     9361 ccacggcacc aggttttcgg cgctcatcag accgaagggg atggagagaa ccaccgccag
     9421 cgcggtgccc cagacggcga tttgcagcgt gacggccatt tcggtgaggt aatcctgcca
     9481 ctggctgaaa tcgggcggga agaagtcggc ggcgaacgtc gccatgttgc cgccgtcttt
     9541 aatcagcgtg agcggggcca tttccgcgcc ctgccacgag acgaccagca cggcgagaac
     9601 aatggcccag ctcagaagcg agaaccagct gcgcttgggt ggggcgatgg tgatggtttg
     9661 catatattgc tccgtttaat gttttctccc tctccctgtg ggagagggcc ggggtgaggg
     9721 catcagcgtg cgcatttccc cctcacccta accctctccc tcaagggaga ggggaccgat
     9781 cgagcacatt actgcaccgc tttactcacc gaactcatcg cgcttaacgc gttgttcagg
     9841 cggtccaggt catccagctg cgcctgaatc tcggtggttt ttgccagctt gtcctgctca
     9901 ttcagccctt tattgctttt cacgccctgc atctctttaa acagtgcgag ctggcgaatc
     9961 ggcaccagtt gcaggtcgct ggaggcgcgg aacggggccc agcccaggcg ttccagcacc
    10021 gctttctctt ccggcgtttt gccgtaattc ataaagaagt cgtagatctt gtctttggtg
    10081 gtttcggaaa gattcttgcg ccagacgatc ggatcgcctg ggatcagcgg cgacttccag
    10141 atcaccttca gttctttcag cttctctggc gcggaggttt tcagcttgtc gaggttttcg
    10201 gtgttgttgg tcgccacatc cacctgcttg ttggcgacgg ccagcgcgtt ggtttcatgc
    10261 ccggcgttga cggtgcgctt gaagtcgctg gcggagatat tgtttttggc gaagacgtag
    10321 tagccgggga cgaggaagcc agaggtggag ttaggatcgc cattgccgaa ggtgagatct
    10381 ttacgcttcg ccagcagatc gttcaggttg ttgatcggac tatctttgtt gacgatcaac
    10441 acgctccagt aacccggcga tccatccgcc gcgaccgtct gggcgaagac ctggccattg
    10501 gcgcgatcca ccgcttccat cgctgacaga ttgccgtacc aggcgatatc cactttgttg
    10561 aagcgcattc cctggataat gcccgcgtag tccggggcaa agaaggcgtt gaccttcacg
    10621 cccagcttct tctccatatc ctgtaagaac ggcgtccatt gcggtttcag gttttgctgt
    10681 gattccgttg aaataatgcc gaaattcagc gccttttcct gctcttcggc gtgcgccggg
    10741 cttaacaggg tgctgaggct gaacatgctg gtgaaggcca gcgaggcaat tatcttagcg
    10801 ttcattcgtt ttcctcaatg atggggatgt caggcagctt tcgcgttttc ttcgacgcgg
    10861 ttgatgctgc ggtagagatg gtcaaaacgt tcgttatcaa actgttggct gctgccgtcg
    10921 tagaagacgt gcccctggcg cagggcgacg atgcgttcgc agtagcgcag ggcgtaatcc
    10981 acctgatgca gcgtgacgac cacggtaatg ccgtcgttct ggttaatgtc gcgcagggtg
    11041 tccatcacga tgcgcgctga ttccgggtcc agcgaggcga tgggttcatc ggccagaatc
    11101 accttcgcct gctgcatcag cgcacgggca atcgccacac gctgctgctg tccgccggag
    11161 agggtggaaa cgcgctgatg ggcaaagtgc accatgccaa cgcgggtcag cgcctgtaac
    11221 gcgcgctgtt tctgctcgcg ggtgaagtag ctaaaacagg tgcgccagaa tggcgtgctg
    11281 ccgagcgcgc caatcagcac gttctccagt acgctcaggc gattcaccag gttgaattgt
    11341 tggaagatgt agccggtgtt ggcgcggctt ttgcggatat cgcgggccag gcggccttcg
    11401 cgttggactg tgcggcccag cagctcgata tggctaccgg cagatttatc gccggtaatc
    11461 aaaccgctta agtgacgtaa aagggtggat ttgccggaac ccgacggccc aagcagagcc
    11521 accatttcac cgtgatgaat gttcagatca accgcgtgca gcgcctgatg ctgattgaag
    11581 gttttggcga gcttctcgac acggataatc gtttgcatga tgcagcctcc ctaaaaaagt
    11641 ggctccatcg tggcggatta ttgtgacgat tttgttaact aaaagtgact aacggatgaa
    11701 gagttaacga gatttcgatg acagtgcggg agggcgggcc tcccggcgga ttattgcgtt
    11761 ggttgttgtt tgacgacatt aatcatccac ggtacgccaa atttatcggt gactttgccg
    11821 aagccatgcg cccagaaagt ttcctgccag gccatttcga tttttccgtt agctgcaagg
    11881 ttatcaaacc agcgttttcc ttcttcgacc tgttgcgaat cgagcaccag cgtaaagccg
    11941 gagtagctgg cttttcctga cggcatggca tcgctcatca tgatgtcgct tccggcaatg
    12001 cgcacattgg catgagcgat ggcggtatcg ggaaattgca ttccggaagg gcagttctcg
    12061 gcgctgtcct gcgctgattt cggcatttcg ccgaagctga ttttatagag cagttccgcg
    12121 cccaacgtac gttgataata ggcaatcgcg tcggaacagt taccggcaaa agagaggtag
    12181 ggacttaacg gcatgatggt gacctcagtt aagagaagcc agttaagtgt agttcgaaat
    12241 ttatacagat gagagggaag cctgataaga cgcggtcagc gtcgcatcag gcagtcggca
    12301 tcgttgccgg atgcggcgcg agcgccttat ccggcttacc gatcacaata caatcagttc
    12361 tttttcacaa actcagattt cagtttcatc ggaccaaaac cgtcgatttt gcaatcgatg
    12421 ttatggtcgc cttcaaccag gcggatgttt ttcactttgg tgccaatttt cagcatcgaa
    12481 gagctacctt tcaccttcag atctttaatg atggtaacgc tgtcgccgtc agccagcaga
    12541 ttgccgttag catctttaac gatcagctcg tcgctttcct gtgcaggttc tgcgtcgttc
    12601 cattcgtagg cacattccgg gcagatgtac atgccgttat cttcgtaggt gtattcggag
    12661 ttgcattttg ggcagtgtgg taatgacatg tggatttcct caaaagtcag cagggcaaaa
    12721 gcgccaaaaa acggcagatt gccgaaaaag gccgcaatta tacacaaaat ccctgagttt
    12781 gtcgggggta ctgctgattt tagacgacga tcgttttcat ttgaaaggaa acgcaaaaag
    12841 aaaataatct ttttgcctcc atttattatt taagataagg ttaatgctct acttgttctt
    12901 tttttaattc aaatgatatt tttatctttc tgacgtttta aacgtatcgg aagacgttat
    12961 ttattctaat aaaaaacaac actctaaata taaaattatt tcttgcagta attataaaag
    13021 ccgaaaacag aactcaaaaa caatctggct aaataaaagt aacaaaattt gctttaagga
    13081 agaattttct atgtacacac agaccctgta tgagttaagt caggaggctg aacgcctgtt
    13141 acagctttct cgccaacagt tgcagttact ggaaaaaatg cctctctctg tccccggaga
    13201 cgatgtgcca caactggctt taccctggag tcagcctaat atcgccgaac gtcacgcgat
    13261 gctgaataat gagttgcgta aaatttcccg actggaaatg gtgctggcga ttgtcggtac
    13321 catgaaagca gggaaatcaa ccaccattaa tgccattgtt ggtacggagg ttttgcctaa
    13381 tcgtaatcgc ccaatgactg cgctgccggc gcttattcgc catatgcccg ggcaaaagga
    13441 accggtactg catttttcac atgtcgcgcc aatcgattgt ttaattcaaa aattacaaca
    13501 gcgcctgcgt gattgcgata ttaagcatct gaccgatgtg ctggaaatag ataaagatat
    13561 gcgtgcgctt atgcagcgga tcgaaaatgg cgtcgctttc gaaaaatatt atctgggtgc
    13621 ccagcctatt tttcattgtc tgaaaagttt gaatgatttg gtgcgactgg cgaaggcgct
    13681 ggacgtcgat tttccttttt ctgcttacgc cgccattgag catattcccg tgattgaagt
    13741 ggagtttgtc catctggcgg ggctggagag ttatcccggt caattgacgt tactggatac
    13801 ccccgggcca aatgaagccg ggcaaccgca tctgcaaaaa atgcttaacc agcagctggc
    13861 acgcgcctcg gcggtactgg cggtgctgga ttatacgcaa ctgaaatcga tctccgatga
    13921 agaggtccgt gaggcgattt tggcggtggg gcaatcggtg ccgctgtatg tgctggtcaa
    13981 taagttcgat caacaggatc gtaacagtga cgacgccgac caggtgcggg cactgatttc
    14041 cgggacgctg atgaaaggct gtattacgcc acagcagata tttccggtgt cgtcgatgtg
    14101 gggctacctg gcgaatcggg cgcgccatga gttagccaac aacggtaagt taccaccgcc
    14161 agagcaacaa cgctgggtgg aagattttgc ccatgccgcg ctcggcaggc gctggcgtca
    14221 tgccgatctg gcggacctcg aacatattcg tcatgctgcc gatcagttgt gggaagattc
    14281 gttgttcgcc aagccaattc aggcgttgct tcatgccgct tacgctaacg cctcgttgta
    14341 tgctctgcga tctgccgcgc ataaactgtt gaattacgcg cagcaggcgc gggaatacct
    14401 ggattttcgt gcgcacgggt taaacgtcgc ttgtgaacaa ttgcggcaaa atatccatca
    14461 gatcgaagaa agtttgcagc tattgcaact caatcaggcg caggtgagcg gcgaaattaa
    14521 acatgaaatc gagctggccc tgacctccgc caactacttt ctgcgtcaac agcaagatgc
    14581 ggtgaatgcg cagttagccg ccttgtttca ggatgattcg gagccgttaa gcgagattcg
    14641 tacccgctgt gagacactgt tacagacggc gcagaacacc atcagtcgcg actttacgct
    14701 gcgttttgcc gagctggaat ccaccctttg ccgggtgtta accgatgtta ttcgccccat
    14761 tgagcaacaa gtcaaaatgg aattgagcga gtcagggttt cgtcctgggt ttcattttcc
    14821 tgtttttcac ggcgcagttc cccacttcaa cactcgccag ctgttcagtg aagtcatttc
    14881 gcgccaggaa gcaacggacg agcagagcac gcgtttaggc gttgtgcgtg agactttttc
    14941 gcgctggttg aatcagcccg actggggacg gggaaatgag aaatccccga cagagacggt
    15001 tgattacagt gtgttgcaac gagcattaag cgcagaagtc gatctttatt gccaacaaat
    15061 ggctaaagtt ctggcagagc aggtcgatga atctgttacg gcaggcatga atactttttt
    15121 cgctgagttc gcttcatgtt tgacggaatt acagacgcgt ttacgcgaaa gcctggctct
    15181 gcgtcaacaa aatgaatcgg tggtcaggct gatgcagcag caattgcagc aggctgtgat
    15241 gactcacagc tggatttaca ccgacgctca gctgttacgc gatgatattc aaacactttt
    15301 cacggcagaa cgatattgat caagacgtta ctggacggcc ccggtcgcgt gctggagtcg
    15361 gttcatccgc gctttttagt ggatctggcg cagggcgatg atgcccgcca tccacaagcc
    15421 catcagcagc agtttcgtga acgactgatg caggaacttc ttgcgcgtgt gcagcttcag
    15481 gcatggacga acggcgggat gttaaatgcg ccgcttagcc tgcgtctgac attggtggaa
    15541 aaactggcgt cgatgctgga tcccggtcat ctggcgctga cgcagatcgc gcagcatctg
    15601 gcgctgctgc aaaaaatgga tcaccgccag cactctgctt tcccggagct cccccagcaa
    15661 attgccgcct tgtatgagtg gttttcagcc cgttgtcgct ggaaggaaaa ggcgttaacg
    15721 caacgaggcc tactggtgca ggcgggtgaa cagagcgagc aaatttttac ccgctggcgt
    15781 gctggggcgt ataacgcctg gtcgttgcct gggcgctgtt ttatcgttct ggaggagttg
    15841 cgctgggggg catttggcga tgcctgccgt ctgggaagcc cgcaagcggt ggcgttgttg
    15901 ctgggtgatt tgcgcgtgaa agcgacacaa catttggcag agagtatcaa tgcggcaccg
    15961 accacgcgtc actattacca tcagtggttt gcctcttcga ccattccgac gggcggggat
    16021 catgctgatt ttttaagttg gctgggaaag tggtccacgg cagataaaca acccgtttgc
    16081 tggtcagtga cccaacgctg gcgaactgtc gcactgggga tgccacgact ctgttcagcg
    16141 cagcgtctgg cgggggcaat ggtcgaggaa atcttctctg taaatctggt gtaaataatc
    16201 agttacacca atgagtccta aacgaaatac atgtgtgaag ttgatcacaa atttaaacac
    16261 tggtagggta aaaaggtcat taactgccca attcaggcgt caactggttt gattgtacat
    16321 tccttaaccg gagggtgtaa gcaaacccgc tacgcttgtt acagagattg catcctgcaa
    16381 ttcccgctcc ccttttgcgg ccgtcgcgct gatttttctg gcgtttgcgg aaatgggcca
    16441 actctgcgag gaaagctatg ctgaaaagga aaaaagtaaa accgattacc cttcgtgatg
    16501 tcaccattat tgatgacggt aaactgcgta aagccattac cgcagcatca ctgggtaatg
    16561 caatggaatg gttcgatttt ggtgtttatg gttttgttgc ttacgcatta ggtaaagttt
    16621 ttttcccggg ggctgacccc agcgtgcaga tggttgctgc acttgccact ttctccgttc
    16681 cctttctgat tcgaccgctt ggcggactct tctttggtat gttgggcgat aaatatggtc
    16741 gccagaagat cctcgctatc actattgtga ttatgtcgat cagtacgttc tgtattggct
    16801 taataccgtc ctacgacacg attggtattt gggcaccgat tctgctgttg atctgtaaga
    16861 tggcacaagg tttctcggtc ggcggtgaat ataccggggc gtcgatattt gttgcggaat
    16921 actcccctga ccgtaaacgt ggatttatgg gcagctggct ggacttcggt tctattgccg
    16981 ggtttgtgct gggtgcgggc gtggtggtgt taatttcgac cattgtcggc gaagcgaact
    17041 tcctcgactg gggctggcgt attccgttct ttattgctct gccgttaggg attatcgggc
    17101 tttacctgcg ccatgcgctg gaagagactc cggcgttcca gcagcatgtt gataaactgg
    17161 aacagggcga ccgcgaaggt ttgcaggatg gcccgaaagt ctcgtttaaa gagattgcca
    17221 ccaaacactg gcgcagcctg ttgacatgta ttggtctggt aattgccacc aacgtgactt
    17281 actacatgtt gctgacctat atgccgagtt atttgtcgca taacctgcat tactccgaag
    17341 accacggggt gctgattatt atcgccatta tgatcggtat gctgtttgtc cagccggtga
    17401 tgggcttgct gagtgaccgt tttggccgtc gtccgtttgt gcttcttggt agtgttgcac
    17461 tgtttgtgtt ggcgatcccg gcgtttattc tgattaacag taacgtcatc ggcctgattt
    17521 ttgccgggtt actgatgctg gcggtgatcc ttaactgctt tacgggcgtt atggcttcta
    17581 ccttgccagc gatgttcccg acgcatatcc gttacagcgc gctggcggcg gcatttaata
    17641 tttcggtgct ggttgccggt ctgacgccaa cactggcggc ctggctggtc gaaagctcgc
    17701 agaatctgat gatgcctgcc tattacctga tggtagtggc ggtggttggt ttaatcaccg
    17761 gcgtaaccat gaaagagacg gcgaatcgtc cgttgaaagg tgcgacaccg gcggcgtcag
    17821 atatacagga agcgaaggaa attctcgtcg agcattacga taatatcgag cagaaaatcg
    17881 atgatattga ccacgagatt gccgatttgc aggcgaaacg tacccgcctg gtgcagcaac
    17941 atccgcgaat tgatgaataa gctgaaacgg atggcctgat gtgacgctgt cttatcaggc
    18001 caattgaagt cttaaggttc acttaatctc tgacgcgcat actctcctcc aggttaacgg
    18061 aggagagtgc aatgaaaaac cgtgtttatg aaagtttaac taccgtgttc agcgtgctgg
    18121 tggtcagcag ctttctttat atctggtttg ccacgtactg atctttcttc agccgtaccc
    18181 aggcccgcgt gccggaagtc tcttgccggt tttgcaggaa aaactgcccg tgatgcaact
    18241 gtgtaatgcg gctgacaata cttaacccca gaccaatccc gccataacgg ctatccatac
    18301 gtacaaacgc tttactcaac tccccgcatt tactctcatc aatacccggt ccttcatctt
    18361 caacggccat gaccgctccg ccatcttctt gcagcttaat cataatgttg ctgccttgcg
    18421 ggctgtaacg atgggcgttt tctaccaggt ttcgcaataa catcctcagc agggttgcat
    18481 caccctgaac ggtgatgtcg gcggcgctct ctggcaatag cagggtttgc tgtcgctggt
    18541 cgagcatggt actgagttcg tcatacgagg ggagaatgac atcttccagc agttttacat
    18601 gttgataatt cccggaagaa aatgactgtc cggcacgcgc cagttgcagc agctgggaga
    18661 cgctctccat catctgatca agtcgagcca ctaacggtgc tacatcaatg tgatgcgttt
    18721 tcgccagcag ttccagatgc aaacgcaccc ccgccagtgg cgttcgcagt tcgtgcgcga
    18781 cgtcagcggt aaacaacctt tcgttatcca gcgtgctggt caggcgactg accagatcgt
    18841 tcaacgccga aaccaccgct tcgatttcga gggtggcgct gtgaatggca ataggcgtta
    18901 agttgtcggc ggtgcgcgct tccagctctt tttgcagctc cgccagcggc cgggtgatgc
    18961 ggcgtaccgc ctgatagcag ataaatagcg tcaggctgac cataaagacg ccggggacga
    19021 tcaggctggc gaccgcctcg cggatctcac gcatgatgtg gcgatcgttg ttgcgattgt
    19081 cgcgtagcgc ctgctcaaac agctgaatct gctcggtact ttcatgccat agccagaaga
    19141 cgctgatcag ctcaaacacc aacaaaatgg ccccaatggt caatatcagc cgttggcgca
    19201 gcgatattgg tcggcgcaga aaacgcatca gattcaatta gttttcctca tttgcgacca
    19261 gcatatagcc aaagccgcgc acggtgcgga tacgggcttt gcccactttg tcgcgcagat
    19321 tgtggatatg cacttccagg gtgttggtcg agggttcatt gtcccagtta tagatgtcgt
    19381 tgtagagaat ttcccgatgc accgggctgc ctgctttaag catcaggcgc gacagcagag
    19441 catattcttt gggcgtcaga atcagttctt ctccgctcat ccatacctgg cggcgaccca
    19501 tattcagcgt cagattgcca acaatcagct cactttcgcc ctgattatta tggcgtcgta
    19561 gcagggcgcg gatacgggcg tgtaactctt ccagcgcaaa aggcttcacc agatagtcat
    19621 cggcaccgac atccagcccg gcgattttgt cggtcagcgt atcgcgagcg gtgaggatca
    19681 gtaccggcag ggtgtatttt ttctgccgga tacgggcgag aaaatgcagt ccatcttcgt
    19741 cgggtaaccc taaatccagt accaccaggc tgtaatgacc ggcctcaagg ctttgttccg
    19801 ccatccgcgc ggttgtcacg ccatcgcacg cgtagccttc ggtttgtgcc gccagaatca
    19861 atccctgcaa taacagcgta tcgtcttcaa caatcagaat tttcattcac tcactctcct
    19921 gcaagtttgc agaatatcat ccgcagcctg gtaatacttc gtctcaacgc cagttaatcc
    19981 caatagcgtg gagaataaat tatcttgtga atagtgttgc gtttgcgcct gtttttgcag
    20041 gcagttctgg tcaacctgat accgtttttg ataatcctcc gacagccaca gcagcattgg
    20101 cacctgtttt tggctatccg gggcgatggc ataaggcaga ccgtgcagat agatgccatt
    20161 ttcacctaac gactcaccgt ggtcagaaag ataaaccagg ctggtggtaa atttatcctg
    20221 atgttctttc agcagattaa tcgctttatc aacaatatag tcgacgtaaa ccagcgtgtt
    20281 gtcgtaagtg ttcaccagtt gctctttggt acaggtctgg atctcgttag tgtcgcaggt
    20341 tggggtaaat ttcctgaact gcggcggata gcggttgtaa taggtcggac cgtggctgcc
    20401 gatggtgtgt aagacaatca cgccatcacc ctgcaggtta ttgatgtact cttccagccc
    20461 gtggaacagc acttcgtcat agcattcgcc gttgatgcac tgaccaggca gattcagcgc
    20521 ggtgacgttc tggtgaggca cgcggtcgca ggcaccttta cagccgccat cgttgtcatt
    20581 ccacagcacg ttgatgcccg ctcgctgaat gatatccagc acgccttcct ggtgctgtgc
    20641 cagctcttct ttgtaatgct cacgcggcat atccgagaac atgcacggta ctgaaactgc
    20701 cgttgccgtg ccgcaagatg cggtattagg gaaatagacc acgttatctt tcgccagccg
    20761 cgggttagtt tcacgcgggt agccgttgag ggagaagttc tccgcccgtg aggtttcgcc
    20821 gacaatcagg atggtcaaat ttttacgttt ctcgttctgc attaacgggt tgcggtgcgc
    20881 gtcttctcca attcgcacca gcggcagatt tgccagtcgc tgatgggagt accatgacca
    20941 gctggcaaca atgctgtttg aggggcttaa ggacttcacc agctctttgt tgttgcggaa
    21001 cagcgaggca tagtctttgt agaacagtgc ggcgaccagc aaaatcagca gtacagaaac
    21061 cagaatattg gccccgcgga aaagaacgct gcgcaggcgt gaggtggtgg gtttgatttt
    21121 aatccagcag gcaatcagcg cagcaagcac gccgctgaat cccagcgtta ataacatttg
    21181 cggtgtcatc aacgcataac tttctgccgg agtggtatca ataatattgg caatcatcga
    21241 gcggtcgatg acgatgccgt aagtcattat gaaatattgt gcagccgcgc cgaccagaat
    21301 aaacaggcag gccagcggtc gattaagcca caagaaagag cttagtgtca ggacaatatt
    21361 aatcacgctg aaggcgacga ctggcatcga caagaaaacc agtacgttat gcagcgagtc
    21421 cagcggcagc gcctgcaaca cctgtttaaa aaaggcaata ttcaggcaga tagagatata
    21481 aaaagcggcc aacaatagcc aggcgagtaa attcaaagag ggtcttttta gtaggcgctt
    21541 caacatcacg gtgtttccat cgaacaaagt gcgcatatgc tcgcaaagca aaattaagcc
    21601 aaccttaagt tcttaaggtt ggcttttatg tttgctggat taaggggaat taatctttgc
    21661 ttattggtgc atctaaggga tacgggtttt tatgcagccg gttgtaattc agcgcataca
    21721 tggcggtgat gaccatcagg gtgacaaatg accacataac ctctttcgcc ccggacccca
    21781 ccacggccca gatgcagtag aggaaggcaa tggtagtaac tgccagatat gccgggcgtg
    21841 ctttaccaaa gtgaccgtgg ccgagcagca gtaacgccgc acaggtgtaa agatatggca
    21901 ccagtgtaaa gatgaccgac acggaagaaa ccagaccgaa ctctttggtc gcgtttggcg
    21961 aaatgctact gagctggaag atggtcatca aaataccgac gataatcagc cctgccactg
    22021 gcgtacccgc tttatttaca cgggcaaaaa tcggtgggaa cagtccgtca tcggcagcgg
    22081 ctttcgccgt ttgacccgcc agcaacgtcc agccgcccag tgaacctaag caacccgcag
    22141 ctgcgcagaa ggaaacaatg gccccggcgg tgtcacccaa cgccatccgt gcggcatcac
    22201 cgaatggcga ggcagaaacg cgcagtgcgg cattagggat catccccata atcgcggtgg
    22261 tggagagcac atagcaaacg gcggcaatca ataccccacc aatggtggcg atagggacgt
    22321 tgcgtttcgg gtttttcacc acacctgcgg caacggaggc actttccaca ccgatgaacg
    22381 accacagcgt aacgttaagg gtactttgaa tcgcgccgaa ggtgcccagg ccgctgacgt
    22441 tccatgccgc catataggtt tcaccacgga accagaacca gccaaatacg gcaatcccga
    22501 cgatggggat cagcgccagc acggtggcaa cggcctgcac gcgggtgatc attttcggac
    22561 cgacaatatt cagcaggacg aagatccaca gcaccacgac gcaggtgatg gttaatacca
    22621 atggatcttt cagaatcggg aagaagtaac ttaaataacc tacgccaatg accaccatgg
    22681 cgatattgcc gatccagcag gccagccagt agaggacgtt ggtttgataa ccgagaaacg
    22741 gcccaaagca gcggcgggcg taagcgtaag aaccaccagg acttgggtcg aggaacgaca
    22801 ttttggcgta taccatcgag agccccagcg caccgataat cgtcaccaac catccataaa
    22861 tggcaatccc gccagtggag gccaggtttg caggtaacag aaaaacacct gaccccataa
    22921 tattccccga caccatcagg gtgacgggga ttaagcccac tttgtgagca tcagcatccg
    22981 aagacataat taaactcctg cgaaggcgag cttcgtgaca ataaattacg tcatatcata
    23041 cgcctgcatt cgatagttga attattatcg accgggttat cgctgatgcc gttattaata
    23101 tagctcttcg ttaaggttaa aaatcaggcg acagtgcgtt ctctgtgctg actaacataa
    23161 tgcagaggcg tcataccgta gaagtcttta aagacagaaa taaagtacga cgtactgttg
    23221 tagccgcagg actgtgatac ctgagagata tttttaccgt ccatcattaa ttcatttacg
    23281 gcataacgca tgcggcaggt ggtgattatt tggctataac tggtgttttc gcttttcagc
    23341 tttttcttta acagactcgg gctaagacat aaacaactgg ctaccatact aagattccag
    23401 tctttgtgga tatcgctttc aataatttga taaacgctgt cacttacaca attacgtaac
    23461 atatacatca ttagtgaaag gaatttttta ctgtcgagaa aacgggatag cacagtgaat
    23521 aataatgcgc gtgttcgttc cttttctgac tcagtttctg cgggcatcat gctgtgttgc
    23581 gcggcaacgc gaaagacgtc tggtgtcagg catggcaggg tgagaatggg cgtagcgcta
    23641 cgttgccata ctggtatttg cgagagatct ttattcagaa accggaggta atctttgatg
    23701 atgtcgtgac taatatgagc aaccaaagtg ttgttaagag aggagacgtc gataatatta
    23761 ttttcacaat ttaataacgc catatggtta gctttaaggc taataggttc tttcccattc
    23821 acccttatcc agacatcttt ttcagtcaat aaaacaatac aaggttggtc gctgcaaatc
    23881 ctcatacatg tactcctgag tgcgaataaa agaccagttc tcgctaaagc aaagcgatac
    23941 cgggaaatgg ttatataatt gtatgatgaa tataaacatg cataaaaata acgagtttta
    24001 ctaatattac aaatttttat agtaatttct catgtacata tttttttaca gggtaaataa
    24061 caggcaaaaa aaagcgcggg gtggggccgc gcaaaaagta tgacattttt gtttgcagca
    24121 atttttatga aaacgttgcc atacttaaaa ggcctcaagc caggcggcgt aatgttattt
    24181 aacaattacg ccttcagcgg aatagtggtt acgctttcac acacataacg tggtaaatac
    24241 cgtcaataat ttctgtccct tctgtttcgt gttcaaaccc agggaaatga tggtcccagg
    24301 attgcagcga gcgtaaataa cttacttgcg gactgttttt atcgccgaag ttttcaccag
    24361 acagcagcat cgggattcct ggcggatacg ggataactga gtttgccgcg atgcgtcctg
    24421 gcagattttc aatggatacc agttcgacat tgttgtcgac aatcgcgttg tacgcttcac
    24481 gtggggtgat ttccgccacc ggcaggccgg aataggcttc gttcaaccgt gcgccagggt
    24541 tgttttcttt cagccaggca aacatggtgt cacccagatc gtgaatcccc atgttcgcgt
    24601 aagtgtcagg atattgttca acaagttccg gcatcacctg cgccagcggt gtgttggcgt
    24661 catagtggcg tttgaaggag caaagggtgt taaccagagt cccccatttc ccacgggtta
    24721 tgcccataga gaacaggaac atgatttgga agtcagtggt gcgggtaggc acaatgccgt
    24781 ggcgaccaag ccaggcagtg accagcgccg ccggaacacc ggtttcttcc agttcaccat
    24841 cttcacccat tcccggagca aggatgctga ctttaatcgg gtcgagcata ctccagttat
    24901 ccggaatatc tttgaagccg tgccagcttt cgcccggatg catgacccag cagtcctgaa
    24961 cggtggtcag cagtttggtt ggtgcgtcag caaagtcata ggttttgccg gtttgtgggt
    25021 cggtgacgac ttctttgttc cacggtttga agaaccagct accgtcagcg gtgaactctt
    25081 tatacagccg cgccatcgcc tgacggaaat caaccgcttc gtcgatcact tcctgggtca
    25141 gtgacaggcc gctgttgccg tccatcatcg acactgccac gtcgttggat gcacagatgg
    25201 catacagcgg ggaggtggtg gcatgcatca tgtaggcctg gttgaagcgg gagaagttaa
    25261 tcgccccacg accttcacgt acatgaatat aagaggcctg tgacagtgca ttcagtagtt
    25321 tgtgggtgga gtgggtggcg aaaacggtag gaccgttgtg atcgccaggt tcgccgcgca
    25381 tggcatagtg atcagcatag atcgggttga aacgtgcata gccgtaccag gcttcgtcaa
    25441 agtgcagacg atcggaggtt ttttccagca gatcctgcgc ttctttagcg ttataacaca
    25501 cgccgtcata ggtacagttg gtcactacgc agtaagacgg tttttgcccg gctttgtctt
    25561 tagtcagcgg gctttcactg attttcttct gcaaggtttc aggttgcatt tcctgcggat
    25621 agattggccc gataatgccg tagcggttgc ggcttggcac catgtagacc ggtttcgcgc
    25681 ctgtcagcat caagccttgt tcgatggatt tatggcagtt acggtcaacg accacgacat
    25741 cgttatcggt catgcaagcc tgcatgatgg tgcggttaga gccggaagta ccgacgacta
    25801 ccgaccagga gcgatcggca ccaaatacgc gtgcggcata tttttcgctt tcgccaaatg
    25861 cgccagtatg gtcaagcaaa gaaccgaggg aagttcgttc gatgcccatg tcggtgcgga
    25921 acagattttc accatagtag tcatggtaga aacgtccggc gggtgttttg gtaaaaccaa
    25981 cgccaccctg gtggcctggc gctgcccagg aatattcatg gatgtcgcta tatttcatca
    26041 gcgcgctgaa cagtggcggc aacagttgct ggcggtagcg ggtcatcgcg gcaacggcgc
    26101 gtccggcgat aaagtcggcg gtatcttcca gaatccaggc gaattcatcg acaagctcca
    26161 gcagatcccg gtccattgcg gcgagggctt tttcccgatc gcccaacagg aagaccggca
    26221 cgttttgttg gcgctcatga agcttaccga tcaattgtct gacgttttga tgttcgtccg
    26281 gatgttccat ttgatagctg aacatcaggc agtcaatggc ttcgtttgaa gagagaatgg
    26341 caaaaccatc atcaaaggag gtggatttaa tcacggtaac attttgctgg cttaaagcat
    26401 ctgccagacg ctcaacggcg ttaccgaccc aggtgtcttg atggagaaac tcgctttcaa
    26461 caattaatac tttcatcatt gcttacccgg ttatgaagga atcttcgttg cgggcaagta
    26521 tcttccggcc ttttcgggct gtaaagcgcg tgaaaaacaa acatttttga aatatttgtt
    26581 tttataaaca atgaattatg tgattatttt aaattatttt cactgcttat tatcgacggc
    26641 taaactattt ttttggctga tactgatatc gtctttagcc gggaaacgtc tggcggcgct
    26701 gttggctaag tttgcggtat tgttgcggcg acatgccgac atatttgccg aacgtgctgt
    26761 aaaaacgact acttgaacga aagcctgccg tcagggcaat atcgagaata cttttatcgg
    26821 tatcgctcag taacgcgcga acgtggttga tgcgcatcgc ggtaatgtac tgtttcatcg
    26881 tcaattgcat gacccgctgg aatatcccca ttgcatagtt ggcgttaagt ttgacgtgct
    26941 cagccacatc gttgatggtc agcgcctgat catagttttc ggcaataaag cccagcatct
    27001 ggctgacata aaattgcgca tggcgcgaga cgctgttttt gtgtgtgcgc gaagttttat
    27061 tgaccagaat cggttcccag ccagagaggc taaatcgctt gagcatcagg ccaatttcat
    27121 caatggcgag ctggcgaatt tgctcgttcg gactgttcaa ttcctgctgc cagcggcgca
    27181 cttcaaacgg gctaagttgc tgtgtcgcca gtgatttgat caccatgccg tgagtgacgt
    27241 ggttaatcag gtctttatcc agcggccagg agagaaacag atgcatcggc agattaaaaa
    27301 tcgccatgct ctgacaggtt ccggtatctg ttagttggtg cggtgtacag gcccagaaca
    27361 gcgtgatatg tccctgattg atactcactt tttcattgtt gatcaggtat tccacatcgc
    27421 catcgaaagg cacattcact tcgacctgac catgccagtg gctggtgggc atgatatgcg
    27481 gtgcgcgaaa ctcaatctcc atccgctggt attccgaata cagcgatagc gggctgcggg
    27541 tctgtttttc gtcgctactg cacataaacg tatctttatt catggatggc tctctttcct
    27601 ggaatatcag aattatggca ggagtgaggg aggatgactg cgagtgggag cacggttttc
    27661 accctcttcc cacagcggcg aggggagcgt ccgagcatca tgaggccgaa aactctgctt
    27721 ttcaggtaat ttattcccat aaactcagat ttattgatgc ttcacgtagg atctgagttt
    27781 atgggaatgc tcaacctgga agccggaggt tttctgcaga ttcgcctgcc atgatgaagt
    27841 tattcaagca agccaggaga tctgcatgat gtctgcaccc aaaattacat ttatcggcgc
    27901 tggttcgacg attttcgtta aaaatattct tggtgatgtg ttccatcgcg aggcgctgaa
    27961 aacggcgcat attgccctga tggacattga tcccacccgc ctggaagagt cgcatattgt
    28021 ggtgcgcaag ctgatggatt cagcaggggc cagcggcaaa atcacctgcc acacccaaca
    28081 gaaagaagcc ttacaggatg ccgattttgt cgtggtggca tttcagattg gcggttatga
    28141 accttgcacg gtgactgatt tcgaggtctg taagcggcat ggtctggaac aaaccattgc
    28201 cgatacgttg gggccgggcg gtattatgcg cgcgttacgt accattccgc atctgtggca
    28261 aatttgcgag gacatgacgg aagtctgccc cgatgccacc atgctcaact acgttaaccc
    28321 aatggcgatg aatacctggg cgatgtatgc ccgctatccg catatcaaac aggtagggct
    28381 gtgccattcg gtgcagggaa cggcggaaga gctggcgcgt gacctcaata tcgacccggc
    28441 tacgctgcgt taccgttgcg caggtatcaa ccatatggcg ttttacctgg aactggagcg
    28501 caaaaccgcc gacggtagtt acgtgaatct ctacccggaa ctgctggcgg cttatgaagc
    28561 agggcaggca ccgaagccga atattcatgg caatactcgc tgccagaata ttgtgcgcta
    28621 cgaaatgttc aaaaagctgg gctacttcgt cacggaatcg tcagaacatt ttgctgagta
    28681 cacaccgtgg tttattaagc caggtcgtga ggatttgatt gagcgttata aagtaccgct
    28741 ggatgagtac ccgaaacgct gcgttgagca gctggcgaac tggcataaag agctggagga
    28801 gtataaaaac gcctcccgga ttgatattaa accgtcacgg gaatatgcca gcacaatcat
    28861 gaacgctatc tggactggcg agccgagtgt gatttacggc aacgtccgta acgatggatt
    28921 gattgataac ctgccacaag gatgttgtgt ggaagtggcc tgtctggttg atgctaatgg
    28981 cattcagccg accaaagtcg gtacgctacc ttcgcatctg gccgccctga tgcaaaccaa
    29041 catcaacgta cagacgctgc tgaccgaagc cattcttacg gaaaatcgcg accgtgttta
    29101 ccacgccgcg atgatggacc cgcatactgc cgccgtgctg ggcattgacg aaatatatgc
    29161 tcttgttgac gacttgattg ctgcccacgg cgactggctg cctggctggt tgcaccgtta
    29221 aaacgcgact aaacgctact gcgccggggg atttattccg gcgcacacct ctgacgatac
    29281 caataacaga aggcgggcgt tggtaacagc gacccgatac cctatgagca tttcaatgac
    29341 tacaaaactc agttatggat ttggagcgtt cgggaaggat tttgcgatcg gcattgtgta
    29401 tatgtacctc atgtattact acaccgatgt cgtcgggctg tctgtgggtt tggtcggtac
    29461 tttgtttctg gtggcgagga tctgggatgc tattaacgat ccgattatgg gatggattgt
    29521 aaatgctacg cgatcgcgat ggggtaagtt caaaccctgg atcctgatcg gtacgttggc
    29581 aaactctgta atcttatttc tcctctttag tgcgcatctg tttgaaggta ctactcagat
    29641 tgtctttgtt tgcgtgacct acatcctctg gggcatgact tacaccatta tggatattcc
    29701 cttctggtcg ctggttccaa ccataacgct cgataaacgt gagcgcgaac aactggttcc
    29761 ttatccgcgt ttttttgcca gtctggcggg ctttgttacg gcaggtgtga cgctaccatt
    29821 tgttaattat gtcggcggtg gcgatcgggg atttggcttt cagatgttca ctctggtact
    29881 gatcgccttt tttattgttt caaccatcat cactctgcgc aatgtgcatg aagtcttttc
    29941 gtcagacaat caaccgtctg ctgaaggaag ccatctgaca cttaaagcca tcgttgcgct
    30001 tatttataaa aacgatcagc tttcatgcct cttgggtatg gctcttgctt ataatgtagc
    30061 cagcaatatt attaccggct ttgctatcta ttatttctca tatgttatcg gtgatgcgga
    30121 tttgttcccc tattatctgt cgtatgcggg agctgctaac ctggtgacgt tagtattctt
    30181 cccacgctta gttaaatcat tatcccgacg cattttatgg gccggagcat ctattcttcc
    30241 ggtgttaagc tgtggtgttc tcctgttaat ggcattaatg agctatcaca acgtcgtcct
    30301 cattgtgatt gcgggtatct tgctgaatgt gggaacggcg cttttctggg tattacaggt
    30361 catcatggtg gcagataccg ttgattacgg tgaatataaa ctgcacgtac gctgtgaaag
    30421 catcgcttac tccgtgcaga ctatggtggt gaagggcggt tcagcctttg cggctttttt
    30481 cattgcggtt gtgttaggga tgattggcta tgtaccgaat gttgaacagt ctacgcaagc
    30541 cctattaggt atgcagttta ttatgattgc tctaccgact ctgtttttca tggtaacgct
    30601 gattctctac ttccgtttct atcgcctcaa tggtgacacg ctgcgcagga tccagatcca
    30661 tctgctggat aaatatcgca aagtaccgcc cgagcctgtt catgctgata ttccggtcgg
    30721 tgcagtgagt gatgtgaaag cctgacgtgg acaatagcta acgaaaatgg cctgatgcga
    30781 tatgtttatc aggccatgtc ttaatgtaat gctttggatt tgcaaatttt gtagaccgga
    30841 taaggaattc acgccgcatc cggcgtcaac aaagcgcacg ttgttatccg gttatcaagc
    30901 caaagcgccg tagctggcgg caatgaaagc gagcgagaaa tatcaccgtc gttaatgcgc
    30961 caagcagcgc gcagaacata tcggattgcg tgtcccactg gtcgccctgg gtgccgagaa
    31021 aatcatccgc tccctggccc attgccagcg ccgcccacca ctcgattaat tcatacatcg
    31081 cgcttatcgc cagcgccacg cagcagacca gaaacgccac catcttatgt ccgcgaacgt
    31141 acatcccgcg cacaaggatc tctcgtgcca ccagtgcagg cactagcccc tggaaaaaat
    31201 gccccagttt gtcatacgga ttacggctta accccagcca ttcctgcacc tcaaaaccaa
    31261 cggggacttt cgcgtaggtg tattgtccgc cgaccatcag gatgatggcg tgaaagaaaa
    31321 tgagcgtata aagcagcggc gttaacggat aacgtttagc ggtggcaagc agtagcggca
    31381 cgacaataat caccggtgtc acttccatca gccaggtgag tttgtcatgg gccgaaatgc
    31441 cggtataaat caggattaac gttagcgcca gtgcgccggt gttaagaatt aacggcttga
    31501 gtgtgcgggt catggtgagt tcgcaagtca gggaaaatac tgactattca ccagcaagcg
    31561 tgaaattaca atcgcaaaag aggtggccca gggggatcac ctggcagcat gctgccaggc
    31621 gctgggccga agaggttact tagtgcagtt cgcgcactgt ttgttgacga tttgctggaa
    31681 gaagtcgttg cctttgtcat cgaccaggat aaacgccggg aaatcttcta cttcgatttt
    31741 ccagatagct tccataccca gctccggata agcgacgcat tccagatgct tgatgctctg
    31801 ctgcgccagt accgccgccg gaccgccgat actaccgagg tagaagccgc catgtttatg
    31861 acacgcgtcg gtaacctgct gactgcggtt acctttcgcc agcatgatca tgctgccgcc
    31921 gtgggattgc agcagatcca cgtaggagtc catacgacct gcggtggttg ggccaagtga
    31981 acctgatgga taaccggcag gcgttttcgc cggacccgcg tagtagatcg ggtgatcttt
    32041 gatgtactgc ggaagttctt taccggagtc aatcagctct ttcagcttgg cgtgtgcaat
    32101 atctcgaccc acaataatgg tgccggtgag cgacaaacga gtggaaaccg gatattgcga
    32161 aagctgggcg aggatctctt tcatcgggcg gttaaggtcg actttcaccg cttcgccttc
    32221 accggcctga cgcagttctt gtggaatgta ctggcctgga ttgtgttcca gtttttcgat
    32281 ccagatacct tcgcggttga ttttcgcttt aatgttacgg tcagcggagc aggagactcc
    32341 catgcctacc gggcaggatg cgccgtgacg tggcagacgg ataacgcgaa tgtcgtgcgc
    32401 gaagtattta ccgccaaact gcgcgccaag accgagtttc tgggcctctt ccagcagttc
    32461 ctgttccagt tggacatcgc ggaacgcctg accatgttcg ttcccttccg tcggcagttc
    32521 atcataatag tgagcgcttg ctaacttgac ggttttcagg ttggtttccg cagacgtacc
    32581 gccaatcaca aacgcgatat ggtacggcgg gcaggctgca gtaccgaggg tacgcatttt
    32641 ctcaacgagg aagtttttca gtttgccggg agtcagcagg gctttggttt cctggtagag
    32701 ataggttttg ttggcagagc cgccgccttt agcgacacag aggaatttgt actcatcgcc
    32761 atctaccgcg tacaggtcga tttgcgcagg caggttagtg ccggtgttga cctctttgta
    32821 catgtccagc gccgcgtttt gcgagtagcg caggttatct tcgatatagg tgttgtacac
    32881 gcctttcgac agtgcttctt catcaccgcc gccggtccac acgcgctgac cttttttacc
    32941 gacgatgatt gcggtgccgg tatcctggca ggtcggcagc acgcctttgg cggcgatttc
    33001 ggagtttctt aagaattgca gcgccacgta cttgtcgttt tcgctggctt ctggatcgtg
    33061 aagaatagcc gcaacctgtt tctggtgtgc cggacggagc atgaaagaag cgtcgtgaaa
    33121 ggcttgctgc gccagcaggg tcagggcttc tggttccact ttcagaatgg tttcgccgtc
    33181 gaagtcggca acgctaacgt aatcggaagt gagtagatag tattcggtat tgtctttccc
    33241 catcgggaaa ggtgcctggt agataaaggg tttgtttgac atagcttcca gcctgtaact
    33301 ctgtatttgt tattcgaaaa tggcgtgccg cgtagtcacg ggcggcacgc aaagtgcatt
    33361 tataagaacc cgtacatcgc ggcgaagatc cagccgaaga cgcacgatac gctcacacca
    33421 atcaaccccg gcagaataaa gctgtggttg atgacgaagc gaccgatgtg ggtggtgcca
    33481 gaacggtcaa actgaatcgc tgccagatcg ctcggataag tcggcaggat gtaataaccg
    33541 tagcaagccg gtgctgaagc cacgatgtat gccggatcaa cgccgatcgc cagcgcgacc
    33601 ggaacaatcg ccgccagcgc cgcagcctga gagtttacaa acttggaaac cagcagcaga
    33661 acaatggcat aggcccacgg atactctttc accatttcgc ccagtacgcc ctgaatttca
    33721 gacatatgcg caccgaacat ggtttctgcc atccatgcga taccgtacac cgccacgatg
    33781 gcgatcatac cggaacggaa gacttcgttt tttgagatag acgcgggatt ggttttggtc
    33841 aggataataa tcagcgcccc ggtcagcagc ataaacatct gaataaccag taccatcgac
    33901 agcggtttgc caccgaagga tggacgcagg tccgaatcag caccaagaag ggcgactaca
    33961 gcgattgccc cgaggaagat ccacattgcc agccagttgc ttttcggcag ttttttatcc
    34021 agcagcgtcg cagtatcacc gtaaacatac tcacggtttt ccggtacgga gatgaatttc
    34081 tggaactctt cgtctttatc cagatcttta ccgcggaacc agctgaagat accgatcgcc
    34141 aggataccga ttaacgtcga tggaatggtg attgccagca ggtcgaggaa ctcaagatgg
    34201 cgaccatcaa aggtgacatt acccagcatc gcaaccagag acacgactgc aaccgacacc
    34261 ggactggcga taatccccat ctgtgcaccg atagaacttg ccgccatcgg gcgttccgga
    34321 cggatgttgt tcttaatggc gacgtcgtag atgatcggca gaatggtgta aaccacatga
    34381 cccgtaccgc aaagaatggt cagcgtacag gtcacaaacg gcgcgacaat tgagacatat
    34441 ttcgggttgc ggcgcagcag cttctcggca atttgcagca tgacatcaag accgcccgaa
    34501 gcttgcaagg tcgccgatgc cgccaccacc gcaatgataa ccagcatgac atcaaccggt
    34561 ggtttacctg gctgaaggtg gaagacgaag accagaatga ccagaccgat accgcctaat
    34621 aaacccagcg cgataccacc ctttctggca ccataaaaca gacatatcag tattatgata
    34681 agttggatag taaataacat gtgtgaaccc tcgcgataat cctatttaaa tttttgctga
    34741 atagatcaca gtcacgttct gttttgtatg aactgttttc agagcctgac taaatatccg
    34801 tttggtcgtt accggcttta gcaaatacct cacagtgaat attggctgat taaagcagcg
    34861 gcaaatttat gcactgtgat aagcggcttt ttcagagaga aaaactctct ggcgatgggt
    34921 ttatagtttg tactgcgcag atgttaatgt tttgtttgct gttatcttat tgatatttat
    34981 cggtctattt tttgttctca gggcggatgt cattaggttt atagattaat ctgatctacc
    35041 catttgtggg taaaaataca cataatgcgg gtgacataat agttaattaa cttttgttag
    35101 cgttttgaaa ttaaaaacac cgttcacctg aagagatatt aatttttagc gatgatggag
    35161 ggataattat atttgatctg gcacaagttt tactgatgaa ggatgtaact tgtgccaggg
    35221 gtattttgca ttacggtaat aattattact tacagataac cagcgatccg ttattggcaa
    35281 tattgtttca gcagtgagta gtgttctgcc tgaatacggt aacggtaaac cggacgcccc
    35341 gtgacgccat aatggatact ggtgaacaag atgtggcagt tgaccagcca gatgaggtat
    35401 ttacggcagg aaacacgcga aatgttaacc tcgttagcca gctcgtcggt tgaaaactca
    35461 tagtcctgat gcgcgtcaat ccactggcac agcgtgcgta acgtctgcgg cgttaagcct
    35521 tttggcaagc gacgaggatc ctgctcgttg gagctgctgc cgtggattag ctgatcaagc
    35581 tcggcctggt cataatactg atgtttttcc agcgccattt tcttttgccg ccagccggtg
    35641 agcgcctctt caaagcggga agcctggaag ggtttgatca ggtaatccac gacaccgtaa
    35701 tgcagcgaat ctttaatggt tgccgcatcg gctgcggagg agatgacaat cacatcactt
    35761 ttgcaacgcg cgttatgcag gacaggcagt aaatcgagcc cgttctcttt ttgcatatag
    35821 atatcgagca atatcaggtc gataggcgta tcgctattga agataatctc tttggctttc
    35881 tccagcgtcg aggctgttcc acagcattga aagcctggga tttgtgctac gtatcggcga
    35941 ttcagctccg cgaccattgc gtcgtcatcg ataattaata cattgatcat ctgttcgacc
    36001 tctccccgtc ccagggtatc tggacaaaaa attgtgtgaa aatcccgggt tccgattcca
    36061 cggcgatgct gccgccgaga ttttctacct gttgtttgac aagtgctaaa ccgacgcctc
    36121 gctcgcttcc ttttgtcgag acacctttgt caaaaatgtg atcgatttta tcgggtgcga
    36181 tccccggtcc atcatcatta acttcacagt gcagccagcc gtgacggtag tgcaatgtta
    36241 cgctaatttc accaccgggt tccggcccta atgcttccag cgcgttttct atcagatttc
    36301 ccaacgtggt aatcagcgtc gcgacctggt cctcactgcc gctgtctggt agctggcttt
    36361 cactgtttaa aatcagcgta tggcctaaat cggtcgcgcg gttaatcttg ctgattaaaa
    36421 aaccagcgat aaccggagat ttgatcttac ccagcagaga gccaatctct tcctgatagt
    36481 tattggctgt tttgagaatg taatcttcca actgcttata actcttcaga tgcaataatc
    36541 cgagaatcac gtgcaattta ttcataaatt cgtgggatcg ttcacgaagt gcgtcagcat
    36601 agttgaccag accatcgagt cgctgcatca gtttacgtac ttcagttttg tccctgaagg
    36661 ttgaaatggc accgatgata acgccattac tgcgcaccgg aacggtgttg atcagtaata
    36721 gccgatcttt aatcgtaatc tcttcgtcgc ggcgcggggt accgtcgcgt aacacttccg
    36781 agacatctac cacctgtgac cacgagtggc ttagcgtcga cagtttctca tcgtcctgcg
    36841 acttacggta attcagcaat tcttgtgcgg catcgttgat cagcgtgact tcaccgcgat
    36901 cgtccacggc aacgacgcct tctttgatag actgcaacat ggcctggcgc tgctcaaaca
    36961 gcgtggagat ttcgtagggt tccaggccaa aaaggatttt tttcagtact ttaaccagaa
    37021 tgcaggtgcc aatcagtccg accagcatgc caaataatac cgaccagata atgctccagc
    37081 gactgtcatt gatctgttgg gtcacgcggc ttaactcaag gccgatcgcc accacgccaa
    37141 tttgcttatg attttcatcg tagatggggg taaatacgcg taaagcctgc gccagaaaac
    37201 cgcgattgat agcgacattt tcttcgccat tcagcgcttt aaggatgtca tcacctttaa
    37261 atggctgacc aatacgctgg gcttcaggat gcgagtagcg aagactttgc atatcggtaa
    37321 cgacaataaa cagcagatcg ttgcgtttgc gcacggcttc cgcgatggcc tggatgccgc
    37381 tctcctgcgg ttttttctgc aagccctgac ggatttccgg cgagtcggcg agagtacgcg
    37441 ccactgccag tgccttgttg gctagcccgt ctcgcgtcat atcactgatt tgcgagaagt
    37501 aaatcagatg caccaccaat agcaccgaga acagtaccgc gctgaccatt aagatcactg
    37561 tggtactcaa tttcatcgga cgtttgcgta acatgcggta gggcaatgaa tgtctcatca
    37621 gcttccttgt gtgacaaatt tcttaagcat tatctctgat gaggcgggta attcaaaggg
    37681 agtaagaatg attggctata taggggaaga gattctggca acggaaactg ccagtgctgt
    37741 atgaagattc cggggctatg cttatagcga taatcatact gatgagagag ggaaggtcat
    37801 ggatcgggcg ctactggacg ggggttatcg ctgttatacc ggcgaaaaga tcgatgtcta
    37861 tttcaatact gcgatatgtc agcattctgg caattgcgta cgtggcaacg gcaagttatt
    37921 taatctcaaa cgaaagccgt ggatcatgcc ggatgaagtc gacgttgcca ctgtggttaa
    37981 agtgattgat acgtgcccga gcggcgcgct gaaataccgt cataaataag cgagggtaaa
    38041 atggaaatac gcgaaggcca caataaattt tacattaatg acgaacaagg caagcaaatc
    38101 gctgaaattg tctttgtgcc gaccggagag aatttagcga ttatcgaaca taccgatgtc
    38161 gatgaaagcc tgaaagggca ggggattggt aaacagctgg ttgcgaaagt cgtggaaaaa
    38221 atgcgtcggg aaaaacgaaa aattatccca ctatgcccat ttgcgaaaca cgaatttgat
    38281 aaaacgcggg agtatgatga tattcgcagt tgatgggaga gtacagattc acgatatttt
    38341 tcatgctctc cgtggggtga tgcaggagag catctgaagg gtagggggat gcacaaagaa
    38401 tggtcagaga gagcgttttt ttgtcccaag tcatcccctt tactgagcaa aaaaaaagaa
    38461 tatctcctat atgagaatca tcgattgggg ttaataagtt ttgcgtcccc agagcgttta
    38521 aaattgatag gagtcatatt atggaaggta aaaacaagtt caatacttat gttgtttctt
    38581 ttgattatcc atcatcttat tcctcagtgt tcttaaggtt aagatcattg atgtatgata
    38641 tgaatttctc ctctatcgtg gctgatgaat atgggatacc acgacaattg aatgaaaatt
    38701 ccttcgcaat aacgacatca ttagccgcaa gtgaaatcga agatttaatc aggctcaaat
    38761 gcttagactt accggatatt gattttgacc tcaacattat gacagttgat gactatttcc
    38821 gtcagtttta caagtagcca ggaaggataa gaagaacata aaaaatggca ctattctcta
    38881 aaatattaat tttttatgtg attggtgtga acatatcctt tgtcattatc tggtttatct
    38941 cacatgagaa aacacatatt cgtttactta gtgcattcct ggtcggaata acctggccaa
    39001 tgagtctgcc tgtggcatta cttttttctc tcttttagga gctgtagttg agtggacccg
    39061 ctggttatct tgaaacgaag cgtctctcct caaaactgga tttgcggaac gacgaacgaa
    39121 gcagtcgcct gactgcttcg ttcatttaag tgaaatttat ttctgtgggc gcatcgccgg
    39181 gaagagaata acgtcgcgaa tagtatggct gttagtaaac agcataatca ttcggtcgat
    39241 accaataccc agaccagcgg ttggtggcaa accatattcc agcgcagtca cgtaatcttc
    39301 gtcatagaac atggcttcgt cgtcacctgc agctttagcg ttaacctgtt cctggaaacg
    39361 ttcagcctga tcttctgcgt cgtttaattc gctaaaacca ttaccgattt cacgaccacc
    39421 gatgaagaat tcaaatcggt cggtgatttc cgggttaaca tcattacggc gcgccagcgg
    39481 tgacacttct gcgggatatt ccgtaataaa ggttggctga atcagatgtg cttctgccac
    39541 ttcatcaaag atctccgtga caatacgtcc cagcccccag cttttctcta ccgtaatacc
    39601 aatagattca gctaatgctt tagcggcatc aaaattatcc aggtcggcca tatcggtttc
    39661 tggacgatat tttttgattg cttcgcgcat ggtgagtttt tcaaacggtt tgccgaaatc
    39721 aaacacatgc tcgccataag tgactttagt ggtacccaga acctcttgtg ccagggtgcg
    39781 gaacagtgac tctgtcagtt caatcaaatc gtggtaatcc gcatacgcca tgtagagttc
    39841 catcattgtg aactcaggat tatggcgaac agaaatacct tcattacgga agttacggtt
    39901 gatttcgaat acccgttcaa aaccgcctac aaccagacgt ttcagataca gctctggcgc
    39961 gatacgcagg tacatatcta aatccagagc gttatgatgg gtaataaacg ggcgagcaga
    40021 tgccccacct ggaattacct gcatcatcgg ggtttctact tccataaagc cgcgcgcgac
    40081 catgaactga cggatagcgg ccagaatttt tgaacggaca acaaacgttt gacgggattt
    40141 atcgttagcg atgaggtcca gataacgttg acgataacgg acttcctgat cctgcagacc
    40201 atggaattta tctggtaaag gacgtagtgc tttagtcagc aggcgcagct cagtacagtg
    40261 aatggaaagc tcacccgttt gcgtcttaaa cagcgtaccg cgggcaccga taatgtcacc
    40321 cagatcccat tttttaaact gatcgttata aacaccttct ggcaggctat ctcttgcaac
    40381 gtacagttga atacggccac cgacatcctg caacgttaca aaggaggctt tccccatgat
    40441 acgacgggtc atcattcggc cagcaaccga gacttcaatg tttaaggatt ccagttcctg
    40501 gttatccttc gcatcaaact cttcgtgcaa ctggtcagag gtatggtcgc ggcgaaaatc
    40561 attgggaaac gccacacctt gctgacgtag tgccgccagt ttttcgcggc gatttctcag
    40621 ttcatcgtta aaatcaatag cctcattggc tccccgtgtt tctcgttcag acattttggt
    40681 tcctctaaat ccagctttca atttagcgta gataaagaga cagatcggta cactgtatta
    40741 tctgcctcga ctattaataa ctcagtttat cttatcgaaa ctaacgctta cctcaagttg
    40801 atatcactaa taaataacca taaaaatggt ttaaatgact tattaatctc gatttgctag
    40861 tgtgataaac tgaaagttaa tttattcata accaattgtt tttacgacag ttaatcgttg
    40921 ctctcctgta tcatattcgt cggcgtgcta aacagaaaac gggtagcaaa ggccaatacc
    40981 acgatgatcg cgacacaagc caacgtccac tctcccatct gagaaaagaa tcgctggtaa
    41041 gcggcaattg ccataccgct aatttgcgac tctgtcgtct gctgtgccac gacgcctgcc
    41101 agccagttgg cgaccgcgcc tgttgccagc atataaatac cggttaatac gccagacatt
    41161 ttcagacgcg tgatttgcgc aatcgccacc gggtcaataa agagttcagc aaagcccatt
    41221 agcgccagcc cggataccat cacgcccatt gacgcttgac cgccagccgc tgcatgtcgg
    41281 gcatcaaatg ccagcaacat aaagccacaa gccatcagca gtaagccaaa ggcaaacttc
    41341 agccagacgc gcaatgctga attgccgcgg ctttctggcg acgccagcca ggccagaaca
    41401 accccagcga gcatcaccgc aatggcattc accgactgga atagtgctgt aggtacttca
    41461 atattgaatg cctgacgatt cacaaagcga tcgataaaca agctgatggt actgccgccc
    41521 tgttgtgcca gtacccagaa caacgtcccg acaaacatca acaatacaat ttgccaaaga
    41581 gcacggcggt gttcggggaa tttgatcatc atgcgggcaa tgatttgtgc tgcaatgagg
    41641 caaacgatcg ccagcaaata tccggaccag tcgttctcca gcagcagggt aaaaaatact
    41701 ggggctaaac agagcatcac cactaaccag ctccatactg gtaaggcaaa tttgacgcta
    41761 gtgagcgctt tcttatccat actacgcgtg gactggaaat gacgatgacc gcttaagaaa
    41821 atcaacaaac cgataaacat gccgccaccc gcaagggcaa agccaacatg ccatccatac
    41881 cactgagcag ccaggccgca ggcgatgggg gcggcgatag aaccgatatt gcctgcagca
    41941 tacagcagcg aaaaaccgcc atcacgtcga tgatcgttct cgtcgtagag ctcgccaagc
    42001 aaacagctga tgtttgattt gaataaaccg tagccacaaa taatgattgc cagcgccaga
    42061 tacaggctaa aggttgaatt tgtatcaatg cccagcacca catggccaag ggtcattaac
    42121 agcgcgccgg caatcactgc agtgcggttg ccgagcaggc ggtcggcaag ccagccgccg
    42181 agaatagggg taacgtaaac cagagaagca tatgcgctga acaggctgat ggcatggtta
    42241 tcatcaaaac caagctgatg ggtgagatag agaatgagta aggcacgcat gccgtaaaaa
    42301 ctgaagtact cccagatttg gatcgccacg atatagtata tcgcgcgcgg ctgtgagggt
    42361 gttttcatgt gttctcctta tgagcaaaaa agggaagtgg caagccactt cccttgtacg
    42421 agctaattat tttttgcttt cttctttcaa taccttaacg gtatagcggc catcagcctg
    42481 acggtatgca ccgtgaatat cagtttcaaa gcccggatag tgagcgccga tttcacacag
    42541 catctgcagg aactccagaa ccggacggct ttcttcggtg atcatttcac ccggcattac
    42601 cagaggaact cccggcggat atggaaggat catattggcg ttaatacgac cgaccatttc
    42661 gtcgaggtaa acttcttcgg tcataccgtg cagctctttc tggaacgcag catacggagt
    42721 cattaccatg gtcggcagca cttcaaatgc gcgatacatc agatccggca gattgtggtg
    42781 aacaatcagt ttgtggatat tctgagccag ttcctgaata cgcatgtttt catagaattc
    42841 aggatcttca cgatacagag acggcagcat gtttttcaca cgcaggttca ggtcgaacgc
    42901 acgtttgaag tcagtcagag cacgcagcag gctcagtgct ttggtcttat cgataccgat
    42961 gctgaacagg aacagcaggt tatacggacc ggttttctca acaacgatgc catgttcgtc
    43021 gaggtatttc gccacgatgc tggccggaat accgaagtcg ctcatggtgc cgtctttttc
    43081 catccccgga gtcagcaggg tgactttgat cgggtcaaga tacatgtgct cgttatcgat
    43141 gtttttgaag ccgtgccagg tgctgtcaga acgcagcggc cagcattcag tggtatcgat
    43201 atgatccggc tgccatacat caaagaacca gccatcagat tccgttctca gacgtttgat
    43261 ctctttacgg aatttgatcg cacgttcaat ggaaccgttg atcagacgct tacctgcatt
    43321 gcctttcatc atcgccgcag cggtttcagt agacgccacg ataccgtagt gcggagaagt
    43381 ggtggtgtgc atcatgtagg cttcgttaaa ggtttcttcg tttacgtcac ctttaacgtg
    43441 gatcatggaa gcctgagaga acgccgccag cagtttgtga gtagactggg tttcgtaaat
    43501 cactttccct tctacacggc caccgctcat accgcattta ccttcgtaaa tcggtgagaa
    43561 gttggtgtaa ggcacccacg cggagtcaaa gtggatggat ttcacatcca gtgttttctt
    43621 gatgaagtcg gtgttgtaca gcagaccatc ataggtagag ttggtaatta cagcatgtac
    43681 cggccaggtt gcgtttggtg tttctttcac gcgcttagca atggtagcgt gctggaattc
    43741 actctgtggg ataccaccaa gaataccgta agcgttacgg gtcgggcgga aatagattgg
    43801 cgtaacatcg ctcatcatca tcaggtgggt cagcgatttg tggcagttac ggtcaatcag
    43861 aatggtgctg cctgccggag cagagtacat accaacaatt ttgttcgcag tggaagtacc
    43921 gttggtcacc atgtagctgc ggtctgcgtt aaagacgcga gcgatatact gttctgcttc
    43981 tttgtgtgga ccactgtgat ccagcagaga acccagttca gatactgaaa tggaaatatc
    44041 agatttcatg gtgttcggac caaagaaatc atagaacagg ctacctaccg ggcttttctg
    44101 gaatgcagta ccgcccatgt gaccaggagt acagaaagta tatttacctt cacgaacata
    44161 tttaaacagt gctttggtca gcggaggcag aatagtgttg atatattcgt cagtggtctg
    44221 cttgatctta ttagcaatat cttcagcagc acccagcgca tattcaaaga agctaatctg
    44281 taaacgcagg tcattcaggc ttacatcgag agtggaatac gtattagcga acgcgtacaa
    44341 cggcaggttc tcgttcattt tgctaatttc ttcgcacagc tcgagattat atttatccca
    44401 gtcaaaaata acgccgcaca gacgcgcatt gttttcgatc agttttaata agtcgtcacg
    44461 gtcgttcggg taaacaatct ggaagttcag acgttcaagc gcgcgatgaa gttcacggat
    44521 gggttcttct ttaaaataaa cccccatgtg attcaatatt gcaataacgt tcatagtcat
    44581 atctccaggt aaaaaaggcc cctcccaaca catgggacaa aatgaaagga ggagcctcgg
    44641 aaaatacttt taattaatgt gcgttagacg ctgtgtggtt atccattgag tggctctggc
    44701 gctcgtgcat tttgcgagcg tagaacatca ggataatcag gctgacgatg aaggtacctg
    44761 ccagctcgaa ggagcttgcg cccatcagcg cgatgaagca gaacacgcaa cccagtacag
    44821 agcagatcag gctgacaaag ttgcggatgt taacgccttc aaaacgaatc aggtcaacgc
    44881 aagagtagaa gtacggcagc atagtcagca gtactgcgat accggtcagt tcaccgaaca
    44941 ggtcagatgc tttaccaccg gcagagttca ttagagtgat gaggatcatc agggcagtca
    45001 ttttcactgc agccagcagc agaccttttt tcggaatacc gttgctgtcg acttcaccat
    45061 aaactttcgg gaagttaccg tcgttagcgg cacgtacacc tgcctggcct accaacatca
    45121 tccaggagcc cagagaagtc aggcacgcaa aggcggtgaa tgcagaaacc agcggcgcag
    45181 cccagttacc gaggatagtt gaagcactga ttgcaaatgg agcaccggaa gctgccatta
    45241 cagaagacgg atacataccg gaaagcacct gagtcgcagc gatataaaca atacctgcta
    45301 aaccagtacc cagcatggtt gccagcggaa cggtacgttt cgggttttta accataccag
    45361 tacttacagc tgcggattca acacccacga aggcccacag gcagagcaga atacttttaa
    45421 tgatcgcatg accatcggtg gtatccgcag tattccagtt agcagcataa gtcgcagcat
    45481 caaaccaatg ccagccaaca atagctgtca ttaccacagg aataagaacc agcaccagac
    45541 caatagtggt taaacggctt acccaggtac cgccgagcat atttacaaag gtaaataccc
    45601 agacgatagc aatacaggcg atacccgccg gaacaggatc atttaatact gggaagaagg
    45661 tggaaagata agatacagcg gtaataccaa tcgccaggtt accaatccag ttagcatggt
    45721 aataaagaac acctgtctga aaaccaaatg caggggaaat ttctccggca taagcaattg
    45781 ggccaccttg ttgcgggttt tttgttgcca gtcgggcata tacatacgcc agcgacattg
    45841 caccaataat agagataatc caaccccaga tagcaatacc accgatactt gctaggttcg
    45901 caggtaataa tgcaataccg ctccccatca tattaccggc aacaacaccg gtacaggcaa
    45961 atagcccgat cttcttggca gaactcatgc tcttctccta atttcatttt tgaatttgga
    46021 gtccgggtca tgatgtataa ctatttcctg accagaccaa actggcgcta agaatactca
    46081 caaaaaagga ttaatcctaa agattaggtg aaataacaca aaagtttctg taagtgagaa
    46141 cttgaggttt tttattaaca catcaggatc gcaagttgat atcatgaaaa gataaacatt
    46201 caatgtttac aatggattgc gggacatttt ctggttaaat ttatgtaata aaaattatgc
    46261 ggcaaataaa ttgccgcaac atattatacc aacaggaaca tacaaaaact caacaacaaa
    46321 tattgccgag cataaatcaa cccgggagtt acttattctg aagcgagaaa tttgtcgaga
    46381 taaggtacaa cataaggaac agaagtctgg aatataccat tttcaatcca gtaaagggtg
    46441 tttgcccctg ggcgtaaatt aaaggcggtg agatatgcat cagctgcttc ccggttcatc
    46501 cccttcattt cataaacctt gccaagcaat acataattta gccaggacat ttcaagatca
    46561 atgccagtat ttatcgcctg ataagactca tctgttttac cttttaccag agcactgacc
    46621 gcttttattt gatatataat ggacaggtta ttcaattccg gtagtgtaac aatgttatct
    46681 atttctgtgt tcagtgctgc taattgtttt tcatctaaag gatgttgaga atggcgcacg
    46741 atatcaacta atgctttttc tgctctcgcg taggtaaatt ctggggatga ttgaacaatc
    46801 tcacctaata attcactggc acggttcaat gatttatcat cgccatgcag taaataatca
    46861 tgtgcctgat aaaaattagt taataacgca ccacgatgag gcaaaatttg ctggagcatc
    46921 tcctgcattc gttgtggcca cggttggttt aacgcttttg ataaactctc cagtaaatca
    46981 ttttgaatcg ccagctgatt accgttagtg atgacataac gtttatccag catggttgaa
    47041 ccatctgcat tgtctaccaa ttttaccgac ataaagcatt gttgagcacg gtattggcgc
    47101 tgattaacaa acgcaataga taatgtttta ccggaactgc tcggttcatc aatgttgtag
    47161 ttgattttgt catgcaccat aaaggtggag aaggtgttaa gtgatgtcgc caccaaatca
    47221 cccacgccta tcgcgtaaga gagctgatac ggggaactcc agctgttaca acttttattt
    47281 accatattaa tgtcaatatc gcgtggattg agcaaaatac gcgatttgct catcggaaga
    47341 cgtgtatcaa gacttgaaaa cgctaccagt gctacacaga tacctaacga caacaggaaa
    47401 aaaaaccata cccaaaaggt agtgaatcgt ttgcttttaa ctggggattg ttcaggtggc
    47461 gttgcggtgt tttgaatgtt aagactgtgg gagggagaat ctgtgtcagg aaccgcctct
    47521 ggtatagggg gaggcgaaga tagcattatt tcctctccct cttcttcgct gtaccagata
    47581 accggcacca ttaatttata gccgcgcttt ggtacagtag cgatatagac aggactatct
    47641 tcatcattat cttttaatga cttacgtagt tctgagatac tctgcgtcac aacgtgattg
    47701 gtgacaatac ttctcttcca gacattatcg ataagttcat ccctgctaag tacttcgcca
    47761 ctgtgttgag caaagaaaac cagaagatcg attaatctcg gctcaagggt aagttgacgc
    47821 ccattgcggc taatttggtt tatggacgga gtaacaagcc attcgccaac gcgaactaca
    47881 ggttgttgca taatagaaac tcattcgaaa agggaatgat gcaatgataa ttgccacaac
    47941 ctatttttac catctataga tggttttatt tacatattat tggtgaatgc aagacgttat
    48001 ttttaccagc cataaacttc ttgattacat agtattacga aaggatttta ctgagaacca
    48061 gaagtaatat tccttatcat caaaattcat catctttgcc aaagaaaaat gttcagaaaa
    48121 taatccatgg gaaattgccc ggcgcgctta ctatttttat ggattgttag tctttgcatg
    48181 agcaagcgga ctgatacatt tctctttgtt ctcattcaga aaatctcatc agtcggcgtt
    48241 cagaccggtg ttgtgcttta tggatgcgtt acgctcctga tgacgtcatt tgacattcaa
    48301 cagcatcacg gggtcgcacg acatttcacg tcagtcagcg ctatagctca ggaacaaatt
    48361 ttcccgaatt gggatatacc cgcaaaatgc tggtgttgtg gggagaattt ggttgagttc
    48421 ggtagaaatg atttttaggc agaacgctta aatt
//
LOCUS       DAPOZO010000030        46907 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPOZO010000030 DAPOZO010000000
VERSION     DAPOZO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.030"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..725
                     /gene="hchA"
                     /locus_tag="ROI21_001857"
     CDS             <1..725
                     /gene="hchA"
                     /locus_tag="ROI21_001857"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDX5110782.1"
                     /translation="PYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHA
                     AGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVVSLNADSE
                     YAAIFVPGGHGALISLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNG
                     YSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGD
                     SPFAANALGKLAAQEMLAAYAG"
     gene            complement(833..2191)
                     /gene="hprS"
                     /locus_tag="ROI21_001858"
     CDS             complement(833..2191)
                     /gene="hprS"
                     /locus_tag="ROI21_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDX5110783.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(2191..2862)
                     /gene="hprR"
                     /locus_tag="ROI21_001859"
     CDS             complement(2191..2862)
                     /gene="hprR"
                     /locus_tag="ROI21_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDX5110784.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2995..3408
                     /gene="hiuH"
                     /locus_tag="ROI21_001860"
     CDS             2995..3408
                     /gene="hiuH"
                     /locus_tag="ROI21_001860"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDX5110785.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3517..4521
                     /gene="msrP"
                     /locus_tag="ROI21_001861"
     CDS             3517..4521
                     /gene="msrP"
                     /locus_tag="ROI21_001861"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDX5110786.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRGRPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            4522..5157
                     /gene="msrQ"
                     /locus_tag="ROI21_001862"
     CDS             4522..5157
                     /gene="msrQ"
                     /locus_tag="ROI21_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDX5110787.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
     gene            5414..6064
                     /gene="zinT"
                     /locus_tag="ROI21_001863"
     CDS             5414..6064
                     /gene="zinT"
                     /locus_tag="ROI21_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDX5110788.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPIFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(6734..6823)
                     /locus_tag="ROI21_001864"
     tRNA            complement(6734..6823)
                     /locus_tag="ROI21_001864"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6787..6789),aa:Ser,seq:cga)
     gene            6917..7714
                     /gene="mtfA"
                     /locus_tag="ROI21_001865"
     CDS             6917..7714
                     /gene="mtfA"
                     /locus_tag="ROI21_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDX5110789.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7815..7890
                     /locus_tag="ROI21_001866"
     tRNA            7815..7890
                     /locus_tag="ROI21_001866"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7848..7850,aa:Asn,seq:gtt)
     gene            8052..8967
                     /locus_tag="ROI21_001867"
                     /pseudo
     CDS             8052..8967
                     /locus_tag="ROI21_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(9161..10465)
                     /gene="ybtS"
                     /locus_tag="ROI21_001868"
     CDS             complement(9161..10465)
                     /gene="ybtS"
                     /locus_tag="ROI21_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HDX5110790.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(10493..11773)
                     /gene="ybtX"
                     /locus_tag="ROI21_001869"
     CDS             complement(10493..11773)
                     /gene="ybtX"
                     /locus_tag="ROI21_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HDX5110791.1"
                     /translation="MSDVQSNVKPPTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(11766..13568)
                     /gene="ybtQ"
                     /locus_tag="ROI21_001870"
     CDS             complement(11766..13568)
                     /gene="ybtQ"
                     /locus_tag="ROI21_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HDX5110792.1"
                     /translation="MKDNNPADNLAWRVIWRLLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            complement(13555..15267)
                     /gene="ybtP"
                     /locus_tag="ROI21_001871"
     CDS             complement(13555..15267)
                     /gene="ybtP"
                     /locus_tag="ROI21_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HDX5110793.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYVRAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFVDTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            15524..16483
                     /gene="ybtA"
                     /locus_tag="ROI21_001872"
     CDS             15524..16483
                     /gene="ybtA"
                     /locus_tag="ROI21_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HDX5110794.1"
                     /translation="MMESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            16674..22781
                     /gene="irp2"
                     /locus_tag="ROI21_001873"
     CDS             16674..22781
                     /gene="irp2"
                     /locus_tag="ROI21_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HDX5110795.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPVLPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTDNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSLEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHLFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            22869..32360
                     /gene="irp1"
                     /locus_tag="ROI21_001874"
     CDS             22869..32360
                     /gene="irp1"
                     /locus_tag="ROI21_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HDX5110796.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWASEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTANVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGTRVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNKAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLSPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARLQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVMLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESNGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     QQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPSRWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            32357..33457
                     /gene="ybtU"
                     /locus_tag="ROI21_001875"
     CDS             32357..33457
                     /gene="ybtU"
                     /locus_tag="ROI21_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HDX5110797.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            33454..34257
                     /gene="ybtT"
                     /locus_tag="ROI21_001876"
     CDS             33454..34257
                     /gene="ybtT"
                     /locus_tag="ROI21_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HDX5110798.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTALQKQPSTSER"
     gene            34261..35838
                     /gene="ybtE"
                     /locus_tag="ROI21_001877"
     CDS             34261..35838
                     /gene="ybtE"
                     /locus_tag="ROI21_001877"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HDX5110799.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            35969..37990
                     /gene="fyuA"
                     /locus_tag="ROI21_001878"
     CDS             35969..37990
                     /gene="fyuA"
                     /locus_tag="ROI21_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HDX5110800.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHDNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
     gene            38682..39392
                     /locus_tag="ROI21_001879"
     CDS             38682..39392
                     /locus_tag="ROI21_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta-barrel
                     domain-containing protein"
                     /protein_id="HDX5110801.1"
                     /translation="MSTWVTITEAVEITTKAIKQKITPSDIYRHALSGNILLSVYFQS
                     PVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCTEARYICPVQQIID
                     TPLLRKLNQFRTFVRNVRPGDELDVPAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGS
                     LLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPR
                     KWRFDATNSELCAERHEC"
     gene            39319..41440
                     /locus_tag="ROI21_001880"
                     /pseudo
     CDS             39319..41440
                     /locus_tag="ROI21_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(41610..42662)
                     /locus_tag="ROI21_001881"
     CDS             complement(41610..42662)
                     /locus_tag="ROI21_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDX5110802.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKYLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERIFSFSIAENIIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKHS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVLESVIVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            42977..44293
                     /gene="shiA"
                     /locus_tag="ROI21_001882"
     CDS             42977..44293
                     /gene="shiA"
                     /locus_tag="ROI21_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDX5110803.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            44395..45849
                     /gene="amn"
                     /locus_tag="ROI21_001883"
     CDS             44395..45849
                     /gene="amn"
                     /locus_tag="ROI21_001883"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDX5110804.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            46192..>46907
                     /locus_tag="ROI21_001884"
     CDS             46192..>46907
                     /locus_tag="ROI21_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDX5110805.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT        11074 a        13005 c        12082 g        10746 t
ORIGIN      
        1 aaccgtatcg cggtaaacat aaaattctgg tgatcgccgc ggacgaacgt tatttgccga
       61 ccgataacgg aaaactgttc tcgaccggta accatccgat tgaaacgttg ctgccgttgt
      121 atcatctcca tgctgcaggt ttcgaattcg aagtggcgac catttccggt ctgatgacca
      181 agtttgaata ctgggctatg ccgcacaaag atgaaaaagt aatgccattc tttgagcagc
      241 ataaatcgtt gttccgcaat ccgaaaaaac tcgcggatgt tgtcgtcagc ctcaacgcag
      301 acagcgaata tgcagcaatc tttgttcctg gtggtcatgg cgcacttatt agtttaccag
      361 aaagccagga cgtggctgct gctttgcagt gggcaatcaa aaatgaccgt tttgttatct
      421 ccctttgcca cggcccggcg gcttttctgg cgcttcgcca cggcgataac ccactgaatg
      481 gttattccat ttgcgcattc ccagacgccg cagacaaaca aacgccagag ataggctata
      541 tgccgggtca tctcacctgg tacttcggcg aagagctgaa gaaaatgggc atgaacatta
      601 ttaatgacga catcaccggg cgagtacata aggaccgtaa agttctcacc ggcgacagtc
      661 ctttcgcagc gaatgcgttg ggtaaactgg cggcgcagga aatgctggca gcttatgcgg
      721 gttaatccct gttacgtatg aatgtaagcg cataatcaat cgcagccacg ttaatactca
      781 cgacgtggct gcgttttttc atggttgatt tatggcgtac tgaagcccta tgttaatttc
      841 tttgcggtaa cgtaattcgg aacacattat gcttattaaa atagtgatac gtagcacttc
      901 ccccatgcaa ttcggcaatc gctttgacta aagaaagacc aagtccctga ccttcggaat
      961 gacgcgaatt atctccccgc caaaatctac ggaagagttt ttcaggctca ttaattttcg
     1021 ttccaggact ggcgatatca atattaagat agccgttggt atcaagaaaa ctggttatat
     1081 gaatgcgcga tttttctggt gaatatctaa tggcattaac aataagattc gataacattc
     1141 gttgcagtaa aattttatcc gcaaagattt gctgattgca ctcgacctta aagcaaatct
     1201 ctttctcgtc tgaaaggtat tcaagataat ccaacaaatt ttcgacttcc ttattgagaa
     1261 aaagtgagtc cagtttcacc aaaacattgt ttttatctgc ccgggcaaga aacagtatgt
     1321 tctctgttaa ccgcgaaata ttttccagct cttcaatgtt accggcaatt gttttttgat
     1381 attcagcgat acttctggtt tgactgagcg taacctgatt ctgacccagt aatgcattaa
     1441 tgggcgttct aagttcatga gcgagatcgt cagcaaactg acttagacgc tcaaaatctt
     1501 tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt tctctcggta
     1561 atgcgctaac ctcaacaggc tcccggctat cgttataatt cagcgcttcc gttacaccac
     1621 tcaacttttt gatctctcgt aatcccgttc tgattaacag cggacttaat actgagcaaa
     1681 gtacaatggc gacaatgcaa attataatgc tattaatttt atactgttca agcatgttat
     1741 gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact tcatcaatat
     1801 taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg ccagctgcgc
     1861 tgattgtctc agtagcaggt atgttattta acatgtcatc actgacattc gtccggttaa
     1921 caattttgtt gatgctatca ccatgaatga tcaagatatc ctgactaaca tccatcatcc
     1981 ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac tgcttgatct
     2041 gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt gccaggccat
     2101 tatagagagt ccatacaatt ccagcgccag caacagacag cagcaatata aaaagcaagg
     2161 ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga atgaatagcc
     2221 catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt ttgcgcggag
     2281 cctgcgaatg gcgacgtcca ccgtattggt atcactatca aagttgattc cccaaatttc
     2341 actcgcaata accgttctgg ggataatctc gccagctctg gaggccagta gccaaagtaa
     2401 ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctcacac tttgactaac
     2461 agagtccatt cttaagccac tgatttctaa tgttgaattc aaagcgtgat gttgccttaa
     2521 ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa ggttttacca gataatcatt
     2581 tgccccactg tccagccctc tgactctgtc atcgacagaa tctcttgcag taaggcaaat
     2641 aacaggagtt tgctttgctg ttcttaacgt ttgtaagatc tgccagccat ccatacccgg
     2701 aagcataata tccagaatga tcaatgcata atcatcctta agcgcaagat aaagcccatc
     2761 tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct gcgttaccca
     2821 ctcctgggtc ctttgattat cttcaataag taaaatcttc atgaaaataa tatgccataa
     2881 atcatatgtt atagaaacag cctggttcat tacaaaattg taatgctgct gtaaggttac
     2941 cgtggacgct ttttcgctat cctcaaaact cattcacatg acaaggatat aaacatgtta
     3001 aagcgttatt tagtactctc cgtagtaacg gctgcatttt cattaccttc tttggtctat
     3061 gctgcacaac aaaacattct tagtgtgcac attttgaacc agcaaacagg aaaacccgct
     3121 gccgacgtga cagtcactct tgaaaagaaa gcggacaacg gctggttaca acttaatacc
     3181 gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac tgcaactacg
     3241 ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa tcttgaaagt
     3301 ttcttccctg agatccccgt tgaatttcat attaataaag tgaacgagca ttatcatgtg
     3361 cctttattac ttagccaata tgggtattca acctatcgtg gcagttaatg cataaatatg
     3421 taaaagcaga tctctgctac tgttagggag gacacttcct gacagattaa cagtaaacgg
     3481 ctcttgctgg ctaaagacaa aaaagtgtga tggcttatga aaaagaatca atttttaaaa
     3541 gaatcagatg ttacggccga gtcggtattc tttatgacgc gtcggcaggt gttaaaagca
     3601 ctgggcatca gcgcggctgc actttctttg cctcacgctg cgcatgccga tctgcttagc
     3661 tggtttaaag ggaatgatcg cccacccgcc cccgccggaa aaccgttgga gttcagcaag
     3721 cctgccgcct ggcaaaatga cctgccactg acgccagcag ataaagtctc cggttataac
     3781 aacttctatg aattcgggct ggataaagcc gatcccgccg ctaatgctgg tagcctgaaa
     3841 accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac cctcgatcac
     3901 gatgatttaa cccgtcgctt tccgctggaa gagcgtattt atcgtatgcg ctgcgtggaa
     3961 gcatggtcga tggtggtgcc gtggattggt tttccgctgc acaaattgct ggcgcttgcc
     4021 gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacaa tttatgcacc ggaacaaatg
     4081 ccaggccagc aggaccgctt tatcggtggc gggctgaaat atccttatgt cgaaggattg
     4141 cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg tgggtgttta tggcaaggcg
     4201 ttaccgccac aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata tggctttaaa
     4261 gggattaaat cgatagtcag tattaagctg acccgcgggc gtccgccaac cacctggaat
     4321 ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt tgatcacccg
     4381 cgctggtcac aggctaccga acgatttatt ggttcaggcg gcatcctcga tgtacagcgc
     4441 cagccaacgc tactgtttaa tggttacgcc gagcaggtgg catcgctgta tcgtggcctg
     4501 gatttgcggg agaatttcta aatgcgtctg acagcaaaac aggtgacatg gctgaaagtt
     4561 tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat taatcacggt
     4621 ggattgggtg ccgatccggt gaaagatatt cagcatttta ctggtcgcac tgcactgaaa
     4681 tttttgctgg cgaccttgtt aatcacacct ctggcacgct acgcaaaaca gccgttattg
     4741 atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact gcatttaacc
     4801 agttacgcat tgctggagct gggcgtgaat aatctggcgt tattaggtaa ggagttaatt
     4861 acccggcctt atttaacgtt aggtattatc agctgggtaa ttttgcttgc gttagcgttc
     4921 acttcaaccc aggcgatgca gcgaaaactc ggtaaacact ggcaacagtt gcataacttc
     4981 gtctatcttg tcgcgatcct ggccccaata cattatttgt ggtcagtgaa gattatttca
     5041 ccgcagccac tcatctacgc tgggctggcg gtactgcttt tagccttacg gtataagaag
     5101 ttgctttccc tttttaaccg gttacgcaag caggttcaca acaaattatc tgtataactt
     5161 catattgccg acaaagtacg cgttatccct gccggatgcg gcgcgagcgc cctatccggc
     5221 ctacgcagat cacatcaagt tatgtttcgt ttgtcatcaa cctcaaatca tcgacataat
     5281 ttattttatt tcaataagat agataagcac acctgagaga actattctct tgtctcctaa
     5341 gagttgttgc attttgttat atgttacaat ataacattac acatcatata cattaactct
     5401 ggaggaaact gttttggcga ttcgtcttca taaactggct gttgctttag gtgtctttat
     5461 tgttagcgct cctgcctttt cgcatggtca tcactcacac ggcaaaccct taacagaggt
     5521 cgaacaaaaa gctgctaatg gtgtttttga tgatgccaat gtacaaaacc gaacgctcag
     5581 tgactgggat ggagtctggc aatccgttta tcctttactg caaagtggca aacttgaccc
     5641 catctttcag aagaaagcgg atgcagataa aaccaaaaca tttgctgaaa ttaaagatta
     5701 ttatcgcaaa ggttatgtga cagacatcga gatgattggc attgaggacg gcattgtcga
     5761 attccataga aataatgaaa caacatcctg taaatatgat tacgatggat acaaaatact
     5821 cacctataaa tcaggcaaga aaggcgttcg ttacttattc gaatgtaaag atcctgaaag
     5881 caaagcccct aaatatatac aatttagcga tcatataatt gcaccacgaa aatcatctca
     5941 ttttcacatt tttatgggta atgattccca acagtcttta ttgaatgaaa tggaaaactg
     6001 gccaacgtat tatccctatc agttgagtag cgaagaagtg gtcgaggaga tgatgtctca
     6061 ttgatttttt tgcgctgact tcagttattt aacagaacag tgcctggtag taacaacaat
     6121 aatttgccaa gacaaataag taagacaaac tcagacacgc tcatacttga gtcgtgtctt
     6181 ttttattatg tctgtagaaa aaaaatgcga aaaaggcgaa caataaatac acaactcagc
     6241 catacagact ggcagcgcaa atgtttccag gagcaagtca tctgtcatgg taaagctaac
     6301 cagaaatata ggttaatttt ggtgtttaaa ccttttttac ttgttagtat gatacaaagg
     6361 ctttcaaaaa agctgggaaa ttcaacaact cttggaaatt atggactttg tcccgcggct
     6421 ttaatctgac ggccgcgttc cttttttgac tcaattattg accccttctc tacgcaactt
     6481 cagttcccgc cagcaacttt gcggcagttt cataggatca atgtctaaaa gaataatggt
     6541 gaccgataag aaaacgactg aataactgta gattttcgct cgaaaccttc ctgtcagctc
     6601 catagcgaat caagtgctga atgtcacagt atcgaacaga aaacagtgac gatctaaccc
     6661 ttcaagaata ttctacgatt gttctgttta ggaaaagcaa ggcgggaagt caggagataa
     6721 gtcattgata aagtggcgga gagaggggga tttgaacccc cggtagagtt gcccctactc
     6781 cggttttcga gaccggtccg ttcagccgct ccggcatctc tccgttcaga tggttgccat
     6841 gatgccagga aatttggcat tttaacagtc cctgtccgtg caattttgtt caagtgacga
     6901 gtttgcgagc aaaacgatga ttaagtggcc ctggaaagta caagaatcag cacatcaaac
     6961 tgcccttccc tggcaggaag cactatcgat ccccctttta acgggtctga cggaacagga
     7021 acaaagcaaa ttagtcactc ttgccgaacg ttttttacag caaaagcggc ttgttccttt
     7081 acagggcttt gaactggatt cattaagaag ctgccggata gcacttctat tttgcctacc
     7141 cgttctggag ttaggactgg aatggctgga tggttttcat gaagtcttaa tttatcctgc
     7201 gccatttgtg gtcgatgatg aatgggaaga cgatatcggt ctggtgcata accaacgtat
     7261 tgttcagtca ggtcagagct ggcagcaagg gcctatcgtt ttgaactggt tggatataca
     7321 agattctttt gatgcatctg gttttaacct gattattcat gaagtcgctc ataagctgga
     7381 cacccgtaac ggcgatcgcg ccagcggagt tccctttatt tcgttgcgtg aggttgctgg
     7441 ctgggaacac gatcttcatg ctgcaatgaa caacattcag gaagaaatcg aattagttgg
     7501 tgagaatgca gcgagcattg atgcttatgc tgccagtgat cctgctgaat gttttgccgt
     7561 actttctgaa tatttcttta gcgccccaga actttttgct cctcgtttcc cttcattgtg
     7621 gcaacgtttc tgtcaatttt atcaacaaga tcctttgcag agactgcatc acgctaatga
     7681 tacagactcg ttttcggcga cgaatgttca ttaattaaca actttgcaga ttaattaacc
     7741 aattgaaatg gcttatgaaa tttagtgttg acagacaagg taccgctaag taatatgcgc
     7801 tccgttcaca cgattcctct gtagttcagt cggtagaacg gcggactgtt aatccgtatg
     7861 tcactggttc gagtccagtc agaggagcca attttctgtt ttcatgcatc cttgcaaatc
     7921 tttatctgat gttgattcaa caggttagtg tgaaaactct tctcggtgca ttttgatttt
     7981 accctacgca tcgggaaaaa ttggtggtca aatctgggat caggttagtt cgataatgga
     8041 gtgaccccca tatgttcctt accgacgcaa aaatccgcac cctcaagcct tctgataaac
     8101 cctttaaagt ctccgattct cacggtctgt atctgctggt caagccgggt ggctcccgcc
     8161 actggtatct caaagctgga ggttgcgtcc cgcacacggc agcacctgag taacataatg
     8221 cgccatgcgg tccatcagga gttaatcgat acgaaccctg cagcaaacct tggcggcgtg
     8281 accacacctc ctgtcagacg gcactatcct gccctgccgc tggagcggct gcctgaactg
     8341 cttgaacgta ttggggcata tcatcagggc cgtgaactga cccggcatgc cgttctgctg
     8401 atgctgcatg tgttcattcg ctccagtgaa ctgcgtttcg cccgctggtc agagattgat
     8461 ttcacaaacc gagtctggac gatacccgcg acgcgagaac ccattattgg cgtgcgttat
     8521 tccggccgcg gggcaaaaat gcgaatgccg catatcgtcc ccctctcaga acagtccatc
     8581 gccattctga aacagattaa ggatatcacc ggtaataatg aactgatctt ccccggcgac
     8641 cataacccgt ataagccaat gtgtgaaaac acggtcaata aggcactgcg ggtgatgggt
     8701 tacgacacga aaaaggatat ctgcggtcac ggcttccggg caatggcatg cagtgcgctg
     8761 atggaatcgg gtttatgggc aaaggacgca gtagaacgcc agatgagtca tcaggagcgc
     8821 aataccgtgc gcatggctta tattcataag gcagagcacc tagaagcccg caaagcgatg
     8881 atgcagtggt ggtcggatta tctggaagca tgccgagaat cttatgcacc gccttataca
     8941 attggtaaaa ataagtttat cccatagtat actaacgaat ccctcactct ggcaaattac
     9001 caccacctcc acaggaggtg gttctatata gacttttatc aacttcatga cgaaaaacac
     9061 ctattttatc ccaaaagaag acaatagagt cacagaggaa tcaaagagcg actcaattgt
     9121 ctttggacag tctggttgtg aggttagaaa acagttactc ctacaccatt aaatagggcg
     9181 caatgctcgc taatttctcc cgggtttctg tcagttcgcg ttccggcgta gattgcgcga
     9241 tgattcccgc ccccgcctgt atccagcagc gctggctatc ctgaaatacg gaacgcagaa
     9301 ctagcgccgc atcaaagcgc gtatcgtcca gcagcaggat tgcgccggaa taaagctctc
     9361 gcggcgtctt ctcaatttgc ataatcgcgt tcagagcagc atttttaggg atacctgagg
     9421 cggtaatcga cggaaacagc acggtaaacg catcccaggc atccttgttt tccgaaagct
     9481 gaccgctcac gccagacccc agatgctgaa cgctgccgcg ctggcgaacc gacattaaat
     9541 cttcaaccac cacgctgccc ggctggcaaa cagcctccag ttcagcaata gcttctttga
     9601 cagaaaggat atgctcaagc acctctttac tgtcgtgcag cagttctgcc tctttcgcct
     9661 tattatgctc cgggtttccc atgcgatcgc gggtgcccgc aagcggttca gtgaccactt
     9721 tattgcccat cactgacatc accagttccg ggctaaagcc cagcgcttcg cgtccttcct
     9781 gacggaacat aaacgagcgc acaggcgtgt ttgcctgccg cccgtataac agcgtggcgg
     9841 gcatatcaat ccgcgatggc aggggaatgg cgcgcgagac aatcactttg acatactcgc
     9901 cacggcggat ctccgcaacg gcgcgcgcaa cctgttgttt atatgcctca ccattgaggg
     9961 cggtatccac cgccagtggt gcgttctgcg tcgttgtacc ggcctctttt acccactcgc
    10021 acaaacgtcg gcacccgtcg gcggagtccg cataaacggt gacatttccc ttttcaaaaa
    10081 taagttcttc acggggaacg gttaacgtca gcagcggcca ctccccggcg ttaaaggcaa
    10141 tcccccgcgc atgcgccgca aagttgaacc caacctgtcc atatacccga cgtcctttca
    10201 cctgaggatg cgccataaaa cgacgcgcgc agtcggcaat ggaatccacc gcccattttt
    10261 gctccccggc atcatcaata aatgtcgcct gcgttccgtc ggcattaatg tgcagccgtg
    10321 cctggcaccc tttgcctaaa taccaacagg gttgacgctc atagacataa cactcttctt
    10381 ctgcgaactg gcgtaaaacg ccagaaatcg tcggtagcca ttgttcctct ggtaacgcca
    10441 gatgtagaaa ttcactgatt ttcatccctt acctctctgt gttattcccg gatcaatcaa
    10501 ttgcgcttgt catcagttct cttgccgaat gcagccggat ataagcaacc cacgccgcca
    10561 gcaacgtgaa tgccccggca aacaggaagc aggccgcata gccaaaatgt tgagcaacaa
    10621 cgccaccgat aacgcctgcc agaatggcga ttgccgcatc agtacattga aagagggtga
    10681 agtcgacacc ggcctgcaaa ggcgatgaaa gcgacatcag cgtggcgtaa agacagacca
    10741 gcgcgcaggc cagcgaaatg gactggacaa tgaccagaca ctggagaatc tgcagcagca
    10801 gatgacccgg cgccatcatg agcgtcatca ccaccgccag cagcgcgatc ccctggacgc
    10861 cataagccgt caacagcgct ctgccgggtg aggtgtattt catcagcaat ccgccggcca
    10921 gcgttcctgc tatgcccgct gcaatattgc cgccgctgaa caacgcgccc aatgcggaca
    10981 tgctcaaccc atgatccaac aacagcggtg ccagaagagg cagcacaaac cgcatgcctg
    11041 aattcagcat caataccagc agtaagccca ggcgcgcctg cttcctcctt agcgcataac
    11101 ctaacgccgg aacatgcagg atagtcgctg ttcgcgtcgg ctccgtaatg cgccacagcg
    11161 ggagtgacag cgccataatc agcaccgcca tcatcagcat ggcgacaggc cagccggatg
    11221 ccgccgacag catcaggaac accccgccgc cgcacatcat tcccagatag ctgccgccga
    11281 cctgcacgct gtttccccag ccgtaacccg tgcgggtcag ttgatcgaca caaaagccgt
    11341 cgctggcgat atcaatggtg gaggcgaccg tgcctgccac cataaacaga acaaataacg
    11401 cgacgatttg cgtatcgggg aatccccctt ctcgcctaaa ccagccaatc gctgcggcaa
    11461 tagtcaggat cgccgctagc gcgacctgac cacgcaggat taacatgcgg gaacggcgtt
    11521 cctggctacc gggcggaagc cgccagcgct cgatccacgg cgcccaaata aacttcagcg
    11581 cccagggcag cataaacagg gttgtcgcac cggcaagcgc cagcgaaccg ccagcagcgc
    11641 gcaccagcgc gggtaaggac tgcatagaca gcgccgatac cagactctgc gtcacataga
    11701 cgccggcaat agcaaaaatc acccgcccgg tcgtcaacgt cggcggtttc acattcgact
    11761 gaacatcact catgcaccca ctctccagac gcggaaaccc cgtcgtcgcg ccacacgcgc
    11821 gcggccattt gcgcctgcca cagcgcctga taacgcccat gatgtgagag caattgcgca
    11881 tgagtaccct gctcaaccac ctgtccctct tccatcacca gaatgtttcc ggccccggcg
    11941 atggtggata aacggtgagc gatgataatc accgtccggt tgtaaaccag gttatcgatc
    12001 gctttttgca ccgccagctc gctttcaata tccagcgcgg cagtcggttc atcgagaatg
    12061 acgactggcg cgtttttcaa taacgctctg gcaatggaaa tccgctggcg ctcgccgccc
    12121 gatagctggc cgcccatctc tcccattggc gtcagccagc cttgcggaag acgggaaata
    12181 aactcaaggc actgcgccgc gcgggcggcc tcttctacct cctgccgcgt cgcctgtggg
    12241 cgcgcgatac ggatattcgc cagcagcgtg tcatcaaaca gccagacatc ctggaaaaca
    12301 acagaaatca ggctattgag ctgttccggc gtcaggcggc gaatatcgac gccgccaata
    12361 gaaatctgcc cttgctgcgg atcggcatag cgcattaaca gtttggtgac ggtagttttg
    12421 cctgcgccgg aggcacccac cagcgcgctc atactggctg ccgggaacgt caaagaaaca
    12481 tgattaagcg cgtggccgtc gccttcttcg tagcgatagc tgacgttgtc aaagcggata
    12541 tcgtaacgtt ccggcatctc gctttgctct gcgacgggaa gcggtgctat cgccataaac
    12601 tgctcaatcc gttgcagggc gctggcgatc agttccacaa ccgaggtgta gctgataaac
    12661 atcgccatcg gttctgcgaa gcgcataatc atcgcgacgg cggcaatcaa aaaggcgagg
    12721 ttcagagtgc ccgtcactac ccagacgatc ccggataaca ccaccacctg taggcccaac
    12781 tccacgacgc tggcgatcag catcgtcgcg ccagcgccct gacggtgagt gcgggtctgt
    12841 aagttttcca gcgcattgaa atgcgccagc agcgcccggc ttttatcggc atcgctgccg
    12901 caggtgcgca ataccatcat cccctgagca aattcaacga tatcgccgct cagacgctgg
    12961 tgcgcttccc ccagcgtctg catttgtcgc cgcatcgccg ggcggcgcca gtaataaaac
    13021 ggcaccagca gagggaagat cagcaacatc accagcccca gccgccagtc tatccacaat
    13081 gtcgccagcg acgccgtcag cggcgtgaca atggtgagca gcaaaatatt ggctatcgca
    13141 ataacataat tgaggttttc atccacgctg cccagcagca aggcgttcat ttcacccgcc
    13201 ctgccgcgct ggagcttctc cagcggcacg cggcgtaact gctcgccaag tcgcaggcgc
    13261 aactcatggg tggcctgcgc cagatgacca cggtattcaa agcccaggcc ataccagcgt
    13321 agcaccagcg tcacaattgc ggcgacgctg aaggccatag cccagttaag aagttgcggc
    13381 gcgtctcccc gtaacagcgc atcaatgatc ggataaagac aggcaaaggc gatcccctgc
    13441 atgaatgccg ccagcaacag cgccagcata ctgcgtcgca gcatcctggc ctgactgcca
    13501 acgctggaga taagcaggcg ccagatgacg cgccaggcca ggttatccgc aggattattg
    13561 tctttcattt tccaccacct cttcctgtgt tccacccggc acccaatgct gcgcctgctg
    13621 atagtgttgc cagagccgtt gatacaggcc gccctgcgcc aacagttgcg tatggttccc
    13681 catttccctg agctgtccgt cggaaaacag caatatcaca tcggcctgag tcaccattga
    13741 gagacgatgc gcgaccatga tgaccgtccg gccacgcatg gccgccgcga gcgccttgat
    13801 aagcgccgct tcgttttccg ggtcagcaaa cgccgtcgcc tcatcgagca ccaggatggg
    13861 gcgatcctgc aaaagcgccc gggcgatagt aatgcgctgc cgctggccgc cggagagaaa
    13921 taccccacgt tccccgactc gagtgttgta accttctggc agagcgctaa taaaatcatg
    13981 ggcctgcgcc actctggccg ccgctattac cgcctccagc ggcgtatccg gcgcgcccag
    14041 acgaatgtta ttggctatcg tgtcgacaaa aaggaagttg tcctgaaaca caaacgagag
    14101 ttgcttcatc agggtgtccg tctgcatatc acgcagatcc acgccgccaa tacggatatg
    14161 gcctttgtcc gggtcggcgt aacgcagcag caagcgcgcc acggtgcttt ttccggcacc
    14221 gcttggcccg accagcgcca caatttgccc ggcaggcaca tgaaagctca cctcctgcaa
    14281 cgcggcgcca gtacgcgctt gcggataatg aaagctcacc tgctcaaagg taatgctcgc
    14341 ctcttgcggc tgctggtcag actgcggcag cgataactcc ggcatcgcga gaacctgata
    14401 aatacgctga atgcttaaac gcgtttgcgc gaccaggtta ttgagcatca tcattggcat
    14461 tacggcttcg gccattccgc tgcccagcag taaaacggcc acccacgcga taaaatcgaa
    14521 actgccatag tgcaacaggc cgtatcccga ccagatcagg acaaacaggg tcgggagagg
    14581 attcagaatc gagaaggaaa aacgcgctga aaaaccggct ttgcgatacc aggttttgag
    14641 cacatcgacc cactcttcaa gggcgcgttg atagcgcaaa aaactggtgc tgccgctatc
    14701 aaacgttctc accacgggca tggcctgcac gaactcaata accgccgcgc taacctgctc
    14761 gcgcgcctta tgataacgct gagccatatc ttccgaacgc tggcgggcga gcacgagaac
    14821 aaccgatcca aacgccagta cccccagcgt cgcgattgcc agccgccagt ccagccagaa
    14881 caagataacg attgtcgcca gcggcatgat gatcgcgcgg acatagagcg gcgtgctgtc
    14941 ggccacaaaa atatgtaacg acttcacgtc atccagcatc actttcgcca ctgacccgct
    15001 gcccatttgc tgtaacacgc ctggcggaag ctgcaatgct ttacgggcta acctgctgcg
    15061 cagaatgttc tccagatgaa aagccgcgta atgagaggtg ttgaacgcct gtaagcgcag
    15121 aacaaaagtt aagaccacgc agccgatcac gccgcccagc ggccaggccg gaatagcgtc
    15181 aggcgtcgcg cgaatgtcac gcaggctcca cgccagaaaa aggagcgccc ccaggctggt
    15241 taatgccgcc agccccgcca gcgccatagc taaaataacc ttcctgcgaa ccggtgtaat
    15301 cacctgccag agaggaaatc gggacagcga ttcagtattg gatgattgag atgacatggg
    15361 agtaactgaa tttcctgatg aatttttacg gacgccaaaa acgacaattc agggtggcgt
    15421 tctgagaatt aatgtgaata ataaccatta tcaataactc atctacccca ttcggttcta
    15481 tttatacccg cattggtcta agccacaggg agataaccag gtcatgatgg agtcaccgca
    15541 aacgcaatct gaaatctcta ttcaccagtt ggtggtcggg aaacctgcaa acgatggcaa
    15601 tattcctgct cagtgtgaac tgctgcgctg ttcgttgcag gaaggtatgg atattttgct
    15661 ctggaggggc cattttgcgc ggcctgagac gttgcagcta cacgacgatc tgggcaggat
    15721 taacttctcg tgcatcctgg aaggcacatc gcgctttgcg attcagggac tccggcgcca
    15781 caccgactgg gagcttgcgc gcaatcgtca ttacattacc catacgccag actgtcgcgg
    15841 cagcgcctcc tattgtggcc gattcgagag cattaccctc tccttcagcc ctgaaaccct
    15901 cgcgctgtgg gtgccggata ttagcgcggt cattaagaat aagatcgact cccactgctg
    15961 ttgccagcag cagcgttgta atgctgaaac ccacttaacg gctcaggcct tgcgtcacgc
    16021 gctgatgcgc atgcacggcg gcttcagtca tgaacaaaag ccgtcgacgc tatggttgca
    16081 ggggcaaagt ctggtcatgc tcagtctggt gctggatgaa catcgcgaag acgccagttg
    16141 cctctcctgc cacttcaacc cgatggaacg ccagaaactg ctgcgggcga aggatctgct
    16201 gctggctgat ctgacgcaag cgccgggcgt cgcggagctg gccagagagt ccggtctgag
    16261 cgttctcaaa attaagcgag gcttccgcgt tctgttcaac aacagcgtct atggcctgtt
    16321 tcaggctgaa cgaatgcagg aagccagaag acgcctggcc aacggcaata cctccgtcat
    16381 gacggtggcg gccgatctgg gctatgccaa cgccagccat ttctctgccg ccttccagaa
    16441 acagtttggc gtaactcctt cgacctttaa acgcgggatg taaccggggt cgcgcccccc
    16501 taaactcctc cctgacagag gcccggatac cccatccggg cctctgttct ttccgcctgt
    16561 taccaacccg aaacgggcat aaataaaccc gtttcgggta gcatccaatt gttaataatt
    16621 attattctca tatgagtaat gcttttcggt aagacgtgcc atcaggagga agaatgattt
    16681 ctggcgcacc atctcaggat tcgctgttac cggacaaccg ccacgcggct gattaccaac
    16741 aattacgcga gcggctcata caggaactga atttaacgcc gcagcagtta catgaagaga
    16801 gcaacctgat ccaggccggt ctggattcca taagattgat gagatggtta cactggtttc
    16861 gtaaaaatgg ctaccgcctt acccttcgcg agctgtatgc cgcccccacg ctggcggcat
    16921 ggaaccagtt aatgctcagc cggtcgccgg agaacgcgga agaagaaacg ccgcccgacg
    16981 aatcatcctg gccgaacatg accgaaagta cccccttccc attgacgcca gtacagcacg
    17041 cctacctgac gggccgcatg ccggggcaga cgcttggcgg cgtgggttgc cacctgtatc
    17101 aggagtttga aggccattgt ctgacggcgt cgcagctgga gcaggccatc acgaccttgc
    17161 tgcaacgcca cccaatgctg catatcgcct ttcgccccga cgggcagcag gtctggctac
    17221 cgcaacctta ctggaacggc gtcaccgttc atgatttacg ccataacgac gctgaaagcc
    17281 gccaggccta tctggacgca ctgcgccagc gcctgagcca ccgtctttta cgcgtggaaa
    17341 tcggcgaaac gtttgatttt cagctgacgc tcttgccgga caatcgccac cgcctccatg
    17401 tcaatattga cctgctgatt atggatgcct ccagctttac gcttttcttc gatgagctta
    17461 acgccctgct ggccggagaa tcgctgccgg ctatcgacac ccgctatgat ttccgctcgt
    17521 atttgctgca ccagcagaag atcaatcaac cactgagaga cgtcgcgcgc gcttactggc
    17581 tggcgaaagc atcgacgctt ccccccgcgc ccgtcttgcc gctggcctgc gaacccgcca
    17641 cgctacgtga agtccgtaat acccgacgcc gcatgattgt cccggcaaca cgctggcacg
    17701 cctttagcaa ccgggccggc gagtatggcg tgacgccgac aatggcactg gcgacctgtt
    17761 tttctgccgt gctggctcgc tggggcggcc tgacgcgtct gctgcttaac atcaccttat
    17821 tcgaccgcca gccgctgcaa ccggcggttg gcgcgatgct tgccgacttc accaatattc
    17881 ttctgctgga taccgcctgc gatggcgata ccgtcagcaa cctggcgcgt aaaaaccagc
    17941 tcacgtttac ggaggactgg gagcatcgcc actggtccgg cgtcgaatta ctccgtgaac
    18001 tcaaacgcca gcagcgctac ccccacggcg ccccggtggt atttaccagc aatctggggc
    18061 gttccctcta cagcagccgc gcagaatcgc cgttgggcga gccggaatgg ggcatctcgc
    18121 aaacgccgca ggtctggata gatcatctgg cgttcgagca tcacggcgag gtctggctac
    18181 aatgggacag caacgacgcg ctgttccctc cggcgttagt cgaaacattg ttcgacgcct
    18241 actgccagtt gattaaccaa ctctgcgatg acgaaagcgc ctggcaaaag ccgttcgcag
    18301 atatgatgcc cgccagccag cgcgcgatac gcgaacgggt caacgccacc ggcgccccca
    18361 ttcccgaagg cttgctgcat gaaggcattt tccgtatcgc tctgcaacag ccgcaggcgc
    18421 tggcggtaac ggacatgcgt tatcagtgga attatcatga gctgacagac tatgcccgcc
    18481 gttgcgcggg caggttaatc gagtgcgggg ttcagcccgg cgataatgtg gctatcacga
    18541 tgtcgaaagg cgcaggacaa cttgttgcgg ttctggccgt cctgctggcc ggggcggttt
    18601 acgttccggt ttcgctggat cagcctgccg cacggcgcga gaaaatctac gctgacgcca
    18661 gcgtccggct ggtgctcatt tgtcagcacg acgccagcgc cgggtcagac gatattcccg
    18721 cccttgcctg gcagcaggcc attgaggcgg agccgatcgc caacccggta gtacgcgccc
    18781 ccacgcaacc ggcctacatt atctacacct ccggctctac cggtacgccg aaaggggtag
    18841 tcatttctca ccggggagcg cttaacacct gttgcgatat caatacccgc tatcaggttg
    18901 gcccgcatga cagggtgctg gccctctccg ccctacattt tgatttatcg gtttacgaca
    18961 tttttggcgt actgcgcgcg ggcggcgcgc tggtgatggt gatggaaaat caacggcgcg
    19021 atcctcacgc atggtgtgag ctgatccagc gccatcaggt cacgctctgg aacagcgtcc
    19081 cggcgctgtt cgatatgctg ctgacctggt gtgaaggttt cgccgacgcc acgccggaaa
    19141 acctgcgcgc agtgatgctt tccggcgact ggatcgggct tgacctcccc gcccgttatc
    19201 gggccttccg gccacaagga caatttatcg cgatgggcgg cgccaccgag gcgtctatct
    19261 ggtctaacgc ctgcgaaatt cacgacgtcc ccgcccactg gcgctccatc ccttacggtt
    19321 ttccgctaac caaccaacgc taccgggtgg tggatgaaca gggccgggac tgccctgact
    19381 gggtgccggg tgaattatgg attggcggca ttggggtcgc ggaaggctat ttcaacgatc
    19441 ccctgcgtag cgagcagcaa tttttgacgc tcccggacga gcgctggtat cgcaccggcg
    19501 atctcggctg ctactggcca gatggcacaa tcgagttcct cggtcgtcgc gacaagcagg
    19561 tcaaagtcgg aggatatcgc atcgagctgg gcgaaatcga aagcgcgctc agccagctgg
    19621 tgggggtgaa acaagcaacc gttctggcga tcggcgaaaa agaaaaaacg ctggcggcat
    19681 acgttgttcc tcagggcgag gctttttgcg ttaccgatca tcggaacccg gtactgccgc
    19741 aggcgtggca cacgcttgcg ggaacgttgc cctgttgcgc catctcgcca gagatctccg
    19801 cagaacaggt agccgatttc cttcagcatc gcctgctaaa actgaagccg ggtcacaccg
    19861 ctggcgccga tcctctcccc ctgatgaact cactcgctat ccagccgcgc tggcaggccg
    19921 tggtggaacg ctggttagca tttctggtga cacaacggcg actgaagccc gctgctgaag
    19981 gttatcaggt ctgcgctggt gaagaacgcg aggatgagca cccgcacttc agcggacatg
    20041 atttaacgtt atcgcaaatt cttcgcggtg cccgtaacga actgtcgtta ctgaacgacg
    20101 cgcagtggtc gccggaaagc ctggccttta accatccggc cagcgccccg tatattcagg
    20161 aactggcgac aatttgccaa cagcttgcac agcgcttaca gcgcccggta cgcctgcttg
    20221 aggtgggaac ccgcactggc cgcgccgcag aatcgctgtt agcacagctc aacgccggac
    20281 agattgagta tgtcgggctt gagcagagcc aggagatgct gctgagcgcc cggcagaggc
    20341 tcgccccctg gcctggcgcc cgtctgtccc tctggaatgc agacacgctg gcggcgcacg
    20401 ctcactcggc ggacattatc tggcttaata acgccctgca tcgtctgctg ccggaagatc
    20461 ccgggctcct tgcgacatta caacagcttg ccgttcccgg cgcgctgctc tacgtgatgg
    20521 agtttcgcca gttaacgccg tccgccctac tcagcacgct cctgttaacc aatgggcagc
    20581 cggaggcctt gctgcataac agcgccgact gggcggcatt atttagcgcg gccggcttca
    20641 actgtcagca tggcgatgag gtcgcggggt tacaacgctt cctcgtacaa tgtcctgaca
    20701 ggcaggtgcg ccgcgatccc cgtcaacttc aggccgccct cgccgggcgt ctgccggggt
    20761 ggatggtgcc gcaacggatc gtcttcctcg acgccttacc gctgacagct aacgggaaaa
    20821 ttgactacca ggcgctgaag cgtcgtcata cccctgaagc ggaaaacccg gccgaagcgg
    20881 atttacccca gggcgacatt gaaaaacagg ttgccgccct ctggcagcaa ctcttatcaa
    20941 ctgacaatgt caccagagaa accgacttct tccagcaagg cggcgatagc ctgctggcga
    21001 cccgtctgac cgggcaactt catcaggcag gttatgaagc gcaattaagc gacctgttta
    21061 atcatccccg gctggcggat tttgccgcca cgctgcggaa aaccgacgtc ccggtcgaac
    21121 aaccattcgt ccactccctt gaagatcgct accagccctt tgcgcttacc gacgtgcagc
    21181 aggcttacct ggtggggcgt cagccgggct ttgccctggg cggcgtcggc tcacatttat
    21241 ttgttgaatt tgaaattgcc gatctggacc tcacccggct ggagacggtc tggaaccgat
    21301 taatcgcccg ccacgatatg ctgcgcgcca tcgtgcgtga tggacagcaa caggtgctcg
    21361 aacagacgcc cccctgggtg atacccgcac acaccctcca tacgcctgaa gaggcgttgc
    21421 gggtgcgcga aaaactggcg catcaggtac tcaaccccga agtgtggccg gtattcgatc
    21481 tccaggtcgg atacgtggac gggatgcctg cccgcctgtg gctgtgtctg gataacctgt
    21541 tgcttgacgg tctgagcatg cagatcctgc tggcggagct ggagcacggc taccgctacc
    21601 cgcaacagct gcttccgccg ctgcccgtca ccttcaggga ttatctgcaa caaccctcgc
    21661 tacagtcgcc caatccagat tctctggcat ggtggcaggc gcagcttgat gatattcctc
    21721 cggcgcctgc gttgccgctg cgctgcttgc ctcaggaggt tgaaacaccg cgcttcgccc
    21781 gcctgaacgg cgcactggac agcacgcgct ggcatcggct gaaaaaacgg gcggctgacg
    21841 cccatctcac cccgtcggcc gtactgttgt cggtgtggtc aacggttctc tctgcatgga
    21901 gtgcacagcc tgacttcacg cttaacctta cgcttttcga caggcgaccg ctgcacccgc
    21961 aaatcaacca gattctgggc gatttcacct cgctgatgct gctgagctgg catcccggcg
    22021 aaagctggct gcacagcgcg cagtcactac agcagcggct gagccagaac ctcaaccacc
    22081 gcgatgtgtc agccatccgc gtgatgcgtc aactggcgca acggcaaaac gtgccagccg
    22141 ttccgatgcc cgtcgtcttt accagcgcgc tgggctttga gcaggataac ttcctcgccc
    22201 ggcgtaatct gctcaaaccg gtctggggca tctcccagac gccgcaggtc tggctcgatc
    22261 accagattta tgaatccgaa ggcgaactgc gctttaactg ggattttgtc gccgcgctgt
    22321 ttcctgccgg gcaggtggag cgccagtttg aacagtattg cgcattgcta aaccgaatgg
    22381 ccgaggatga aagcggctgg caactgccgc tcgccgcgct ggtgcctccc gttaaacacg
    22441 cagggcaatg cgcagagcgc tcaccgcgcg tatgccctga gcactctcag ccacacattg
    22501 cggcggacga gagcaccgtc agcctgattt gcgacgcctt ccgcgaggtg gttggcgagt
    22561 ctgtcacgcc cgcagaaaac ttctttgagg cgggcgcaac gtcgctgaat ctggtgcaac
    22621 tgcacgtttt gttacaacgt cacgaatttt ccaccctgac gttgcttgac ctcttcaccc
    22681 acccttctcc tgctgccctg gccgattatc tggccggcgt cgccacggtg gagaaaacac
    22741 aacgacctcg ccctgttcgc cgtcgtcagc ggcggatata gcgcgaagca aactgatttt
    22801 ccccggaacg ccatcgcgaa cgcatggcgt tccattgact ttgtgaatct taggaaacgg
    22861 gaccgattat ggataacttg cgcttctctt ctgcgccgac agcagattcc attgatgcat
    22921 cgatcgctca acactacccg gactgcgaac ctgtcgcggt tatcggctac gcctgccatt
    22981 ttcctgaatc gccggatggc gaaacgttct ggcaaaatct gctggaaggt cgtgaatgca
    23041 gccgacgctt tacgcgcgaa gagcttctgg ccgtcggtct ggatgccgcc atcattgacg
    23101 atcctcatta tgtcaatatc ggtacggtgt tagacaacgc cgactgcttc gacgccaccc
    23161 tgtttggcta ttcgcgacag gaagcggagt cgatggaccc gcagcagcgc ctgtttttgc
    23221 aggcggtctg gcatgcgctg gaacatgccg gttatgcccc cggcgccgtc ccccataaga
    23281 ccggcgtttt cgcctcttcc cggatgagta cctaccccgg tcgcgaagca ttgaacgtga
    23341 cagaagtcgc gcaggtaaaa ggtctgcaat ctctgatggg caatgataaa gactatattg
    23401 ccacccgcgc cgcgtacaaa ctcaacctgc acggcccggc gttatcggta cagaccgcct
    23461 gctccagctc gctggttgcc gtgcatctgg cctgtgaaag cctgcgcgct ggcgaatccg
    23521 atatggcggt tgccggcggc gtggcgctct ctttccccca gcaggcaggc taccgctacc
    23581 agcccggaat gattttctct cctgatggtc actgtcgtcc ctttgacgcc tcggctgagg
    23641 gcacctgggc cggtaacggt ctcggctgcg tggtgctgcg tcgcctgaga gacgcgctgc
    23701 tgtcaggcga tccgattatc tcggtgatcc tctccagcgc ggtcaacaac gacggcaaca
    23761 gaaaggtcgg ctataccgcc ccttccgtcg cagggcaaca ggcagtcatc gaagaggcgt
    23821 taatgctggc ggccatcgac gacaggcagg taggttacat tgaaacccac ggcaccggca
    23881 caccgctggg cgacgcgatt gaaattgaag cgttacgcaa cgtctatgcg cctcgcccgc
    23941 aggatcagcg ctgtgcgctc ggttccgtga aaagtaacat gggccatctg gataccgcgg
    24001 cgggcattgc cggactgctg aaaaccgttc tggcagtcag tcgcgggcaa attcctccct
    24061 tactgaattt tcacaccccc aacccggcgc tgaaacttga agagagcccc tttaccatac
    24121 cggtgtcggc acaggcatgg caggacgaaa tgcgctatgc gggcgtctcc tcctttggta
    24181 ttggcggcac caactgccat atgatcgtcg cctcgctgcc cgacgcgctc aacgcgcgcc
    24241 tccccaatac ggatagcggc agaaaaagta ccgcgctgct gctcagcgcc gccagcgaca
    24301 gcgcgttgcg gcggctggcg acggattatg ccggggcgct gagagagaat gcggatgcca
    24361 gctctctggc cttcacagcc ctgcacgcgc gccgtctcga tctccccttc cgcctggcgg
    24421 cgccattaaa ccgtgaaacc gccgaggcgc tcagcgcctg ggctagtgag aaatcggggg
    24481 cgctggttta cagcggccac ggcgccagcg gcaagcaggt gtggctgttt accggccagg
    24541 gctcgcactg gcgcactatg ggtcaaacga tgtaccagca ctcaacggcg tttgccgaca
    24601 cgctggatcg ctgtttttcc gcctgtagcg aaatgctcac gccgtcactg cgcgaagcga
    24661 tgtttaaccc cgattcggcg cagctggaca atatggcctg ggcgcagccg gcgattgtcg
    24721 cgtttgaaat cgcgatggcg gcgcactggc gtgctgaagg actgaagcca gacttcgcca
    24781 ttgggcattc cgtcggtgaa tttgccgctg ccgttgtctg cggacactat acgattgaac
    24841 aggtcatgcc actggtttgt cggcgcggcg cgctaatgca gcagtgcgca agcggcgcga
    24901 tggtggcggt atttgcagac gaagacacgc tgatgccgct ggctcgccag tttgagctgg
    24961 atctcgccgc caacaacggt acgcaacata cggtattttc cgggccggaa gcccgtctcg
    25021 cggtattttg cgccacgctc tcgcagcatg acattaacta tcgtcgcctg agcgtaaccg
    25081 gtgcggcgca ctccgcttta ctggagccga tactcgatcg gttccaggac gcctgcgcgg
    25141 gactgcacgc ggagccgggg caaataccga ttatttccac gctcaccgcc aacgtcattg
    25201 atgagtcaac gctcaaccag gcggattact ggcgccgaca catgcgccag ccggtgcgtt
    25261 ttatccagag tattcaggtg gcgcatcagc tcggcacccg cgtttttctg gagatggggc
    25321 ccgatgccca gttggttgct tgcgggcagc gcgaataccg cgataacgca tactggatag
    25381 ccagcgcccg gcgtaacaaa gaggcgagcg atgtcctcaa tcaggccctg ctccagcttt
    25441 acgctgccgg cgtcgcccta ccgtgggccg acctgctggc gggcgatgga caacgtatcg
    25501 ctgcgccatg ttatccgttt gatactgaac gttactggaa agagcgcgtc tccccggcct
    25561 gcgagcctgc cgacgcagcg ctgtctgccg ggctggaggt ggcgagtcgc gccgcgacag
    25621 cgctcgatct ccctcgcctg gaagcgctta aacagtgcgc cacgcgactg cacgccatct
    25681 acgtcgatca actggtacaa cgctgtaccg gcgatgccat agagaacggc gtggacgcca
    25741 tgaccatcat gcgccgtgga cgtctgctgc cccgctacca gcagctactc cagcgcctgc
    25801 tgaataactg cgtggtcgac ggcgattacc gctgcaccga cgggcgatac gtccgcgccc
    25861 gccccattga acatcaacag cgggaatcac tgctgacgga acttgccggt tattgtgaag
    25921 gttttcaggc tattcccgac accatcgccc gtgccggcga tcggttatat gaaatgatga
    25981 gcggcgcgga agaaccggtg gcgattatct tcccgcaaag cgcctccgac ggcgtggaag
    26041 tgctgtatca ggaattcagc tttggccgct atttcaacca aatcgccgcc ggggtattac
    26101 gcggcattgt ccagacgcgt cagccccgcc agccgttgcg tattcttgaa gttggcggcg
    26161 gaaccggcgg caccaccgcg tggctgctgc cggaactcaa cggcgttccg gcactggagt
    26221 accatttcac cgatatctcg gcgctgttca cccgtcgcgc ccagcagaaa ttcgccgact
    26281 atgattttgt gaagtatagc gagctggatc tcgaaaaaga ggcgcagtct cagggtttcc
    26341 aggcacagtc ttacgatctt atcgtggcag cgaacgtgat tcacgccacc cgccatattg
    26401 gccgcacgct cgataatctg cgccccctgc tcaagccggg cgggcgcctg ctgatgcgcg
    26461 aaatcaccca gccaatgcgt ctgtttgact tcgttttcgg cccgctggtt cttccgctac
    26521 aggatctcga cgcccgcgaa ggtgagttat tcctcaccac cgctcagtgg caacaacagt
    26581 gccgccacgc cggattcagc aaagtggcgt ggctaccgca ggatggcagc ccgaccgccg
    26641 ggatgagcga acatatcatt ctcgccacgc tgcccggtca ggcggttagc gccgtaacat
    26701 tcaccgcgcc atcagaaccc gtgttggggc aggcgctgac ggataacggt gattatctcg
    26761 ccgactggtc tgattgcgca ggtcagcccg aacagtttaa cgcccgctgg caggaggcct
    26821 ggcgtctgct ttcacagcgt catggcgacg ctctgcctgt ggaaccgccc cccgtcgccg
    26881 ccccggagtg gctggggaag gttcgcttaa gctggcaaaa caaagccttt tcccgcggtc
    26941 agatgcgcgt tgaagcccgt catcctgctg gcgagtggct gccgctatcg cccgccgcgc
    27001 ctcttcctgc gccgcaaacg cattatcaat ggcgctggac gcccctcaac gtcgccagca
    27061 ttgaccatcc gcttaccttt agcttcagcg ccggtacgct tgcgcgcagc gacgagctgg
    27121 cgcaatacgg catcattcac gatccgcacg cctcttcacg actgatgatt gttgaggaga
    27181 gcgaggatac gctggcctta gcggagaaag tgatagcagc gctcaccgcc agcgcagccg
    27241 gattgattgt ggttactcgc cgcgcgtggc gagtcgagga aaatgaagca ctctctgcat
    27301 cccatcacgc gctatgggcc ttgcttcgcg tcgcggccaa cgaacagccg gaacggttgc
    27361 ttgccgccat cgatctcgcc gaaaacaccc cgtgggaaac gctgcatcaa gggttgagcg
    27421 cagtctcact atcacagcgc tggctcgccg cacggggtga caccctttgg ctcccttcac
    27481 tgtcgcccaa tacgggatgc gccgctgaat taccggcaaa cgtgtttacc ggcgatagcc
    27541 gctggcatct ggtgaccgga gcgtttggcg gattaggccg ccttgccgtg aactggctca
    27601 gagaaaaagg ggcgcgacgc atcgccctgc tggcgccgcg cgtggatgag tcatggctac
    27661 gcgacgtgga gggcgggcag acgcgcgtct gccgttgtga tgtgggcgat gccgggcaac
    27721 tggccacggt tcttgacgat ctggcggcca acggcggcat tgccggagcg attcatgccg
    27781 ctggcgtatt ggctgacgcg cccttgcagg agcttgatga ccaccagctg gctgccgttt
    27841 tcgcggtaaa agcgcaggcg gcaagccagc tgttgcaaac cctgcgcaac cacgacggac
    27901 gctatcttat tctctactct tccgctgccg ccaccctcgg cgcgccgggt cagagcgccc
    27961 atgcgctggc ctgcggctac ctggacgggc tggcccagca gttttccacc cttgatgcgc
    28021 cgaaaacgct ctctgtcgcc tggggcgcat ggggagaaag cggtcgggcg gccacgccgg
    28081 aaatgctggc gacgctcgcc agccgaggta tgggcgcgtt aagcgatgcc gaaggctgct
    28141 ggcacctgga acaggcggtg atgcgcggcg ccccgtggcg actggcgatg cgcgttttta
    28201 ccgacaaaat gcccccgtta caacaggctc tgtttaacat cagcgccaca gaaaaagccg
    28261 caacgccggt cattcctcct gctgatgaca acgcctttaa cggcagcctg agcgatgaaa
    28321 cagcggtgat ggcatggctg aaaaagcgga ttgcggttca gctaaggctg agcgatccgg
    28381 cgtcactgca tccaaaccag gatctgttgc aactcggcat ggactcgctg ctcttccttg
    28441 aactcagtag cgatattcag cactacctgg gcgtacgcat caatgcggaa cgggcgtggc
    28501 aggatctgtc tcctcatgga ctcacgcagc ttatctgttc taagccagag gcgacgcctg
    28561 ccgcttcgca gccggaagtg ttgcggcacg acgccgacga gcgttatgcg cccttccctt
    28621 tgacgcccat tcagcacgcc tactggctgg ggcgaaccca cctcattggc tatggcggcg
    28681 tcgcctgtca cgtcctgttt gagtgggata aacgccacga tgagttcgat ctcgccatac
    28741 tggagaaagc atggaaccag ctcatcgcac gccacgatat gttgcgtatg gtggttgatg
    28801 ccgacgggca gcagcgaatc ctggcgacaa cgccggagta tcacatcccg cgtgacgatc
    28861 tgcgcgcgct ttccccggaa gaacagcgca tcgcgctgga aaaacggcgg catgaactga
    28921 gctatcgcgt tttgcctgcc gaccagtggc ctctttttga gctggtggtc agcgaaatcg
    28981 acgattgcca ttaccgtctg catatgaacc tcgacctttt gcagtttgat gtgcagagtt
    29041 ttaaagtcat gatggacgac ctggcgcagg tctggcgcgg tgaaacgctg gcgccgctcg
    29101 ctattacctt ccgtgattat gtgatggctg aacaggcgcg cctacagaca tcggcatggc
    29161 acgatgcctg ggattactgg caggaaaaac tgccgcaact gcccttagcg ccagagctgc
    29221 cggtggttga gacgcccccg gaaacgccac acttcaccac cttcaaatcg acgatcggca
    29281 agacagaatg gcaggccgtg aaacagcgct ggcagcagca aggcgtcaca ccgtctgccg
    29341 cgctgctcac gctgtttgcc gctacccttg agcgctggag ccgtaccaca acatttacgc
    29401 tgaacctgac gttcttcaat cgccagccga tccatccgca aatcaaccag ttgattggtg
    29461 attttacctc cgtcatgctg gttgatttta acttctcagc gccggtgacg ttgcaagagc
    29521 agatgcaaca gacccaacag cgcctctggc aaaacatggc gcacagtgaa atgaacggtg
    29581 ttgaggtgat ccgtgagctg ggccgcctgc gcggatcaca acgtcaaccg ctgatgccgg
    29641 tagtgtttac cagtatgctg gggatgacgc tggaaggcat gactatcgat caggcgatga
    29701 gccatctgtt cggcgaaccc tgctatgtat tcacgcaaac gccgcaggtc tggctggatc
    29761 atcaggtcat ggagagcaac ggcgagttga tgtttagctg gtactgcatg gacaacgtgc
    29821 tggaacccgg cgctgccgag gcgatgttta atgactattg cgccatcctg caagccgtca
    29881 tcgccgcccc tgaaagcctg aagactctcg ccagcggcat cgccaggcac attccccgcc
    29941 gacgctggcc gctgaacgcg caggcggact acgacctgcg ggatattgag caggcgacgc
    30001 tcgaataccc cggcatccgg caggccagag cggaaataac cgaacagggc gcgttgacgc
    30061 tggatatcgt gatggccgac gatccttcgc catcagcggc gatgcctgat gagcacgaac
    30121 ttacccaact ggcgctgccg ttgcctgagc aggcgcagct tgatgagctg gaggcgacct
    30181 ggcgctggct ggaggcgcgt gcgctacagg ggatcgcggc tacgctaaat cgtcacggcc
    30241 tgtttaccac gccggagatc gcccatcgct ttagcgcaat agtacaggcg ctgtccgcgc
    30301 aagcgtctca acagcgtctg ctgcgccagt ggctacagtg tctgacggaa agagagtggt
    30361 taatccgcga aggtgaaagc tggcgctgcc gcattccgct cagcgagatt cctgagcctc
    30421 aggaagcgtg cccgcaaagc caatggagcc aggcgctggc gcagtatctg gaaacctgca
    30481 tcgcccggca cgacgccctc ttctccgggc agtgttctcc gctggaattg ctgttcaacg
    30541 agcagcatcg cgttaccgac gcgctgtatc gcgacaaccc cgccagcgcc tgtctgaatc
    30601 gctataccgc gcagattgcc gccttgtgca gcgcagaacg gattctggag gttggcgccg
    30661 gaaccgcagc cactaccgcg ccggtgctga aggccacgcg gaacacgcga cagtcgtacc
    30721 acttcacgga cgtctccgcg cagttcctca atgacgccag agcccgtttc catgatgaat
    30781 cgcaggtgtc ttatgccttg ttcgacatca accagccgct ggatttcacc gcccacccgg
    30841 aggcgggtta cgacctgatc gttgccgtca atgtgctcca cgacgccagc catgtcgtcc
    30901 agacgttgcg cagattaaaa ctgttgctga aagccggcgg acgtttgctg atcgttgaag
    30961 cgacggagcg aaacagcgta ttccagctgg cgagcgtggg ctttattgag ggattaagcg
    31021 gataccgcga tttccgccgc cgggatgaga aaccgatgct cacccgctcc gcatggcagg
    31081 aggttctcgt tcaggccggg tttgcaaacg agctggcgtg gcccgcgcag gaatcgtcgc
    31141 cgctgcgcca gcatctgctg gtagcgcgtt cgcctggcgt aaatcgcccg gataaaaaag
    31201 ccgtgagccg ctatttacag cagcgctttg gcaccggtct gcccatttta cagatccggc
    31261 aaagagaagc gttatttacg ccgctgcatg ccccgtctga tgcgccgact gagccagcca
    31321 aacccacgcc agttgccggg gggaatccgg cgctggaaaa acaggtggct gaactctggc
    31381 aatcgctgct gtctcgcccc gtggcaaggc atcacgactt tttcgaactg ggcggcgaca
    31441 gcctgatggc gacaaggatg gtcgcgcagc taaaccggag agggattgcc agggctaacc
    31501 ttcaggatct gttcagccat tcgacgctga gcgacttctg cgcccatcta caggcggcta
    31561 cgtcaggaga ggacaacccg ataccccttt gccagggcga cggtgaggaa accctgtttg
    31621 tcttccacgc ttcagacggc gatatcagcg cctggctgcc gctcgctagc gcgttgaaca
    31681 ggcgcgtttt cggcctgcaa gcaaaatcgc cgcagcgctt tgccacgctc gaccagatga
    31741 tcgatgagta tgtcgggtgc atccgtcgtc agcagcctca cggcccttat gtgctggcgg
    31801 gttggtcgta tggcgcgttt cttgcggcgg gcgccgcaca acgcctgtac gccaaaggcg
    31861 agcaggttcg gatggtgtta atcgatcccg tgtgccgaca ggatttctgt tgcgaaaacc
    31921 gggcggccct gctgcgcctg ttagccgaag gacaaacgcc tctggcactg cccgaacatt
    31981 tcgaccagca gacgcccgac agccagcttg ccgactttat cagcctcgct aaaacggccg
    32041 gtatggtgtc gcaaaacctg acgctgcaag cggcagaaac gtggctcgac aacatcgcgc
    32101 atctgctgcg tttactgact gagcatacgc cgggcgaaag cgttccggtc ccctgtctca
    32161 tggtgtatgc cgccgggaga ccctcgcgct ggacgccagc agaaaccgag tggcagggct
    32221 ggataaacaa cgccgacgac gctgtgattg aagccagcca ctggcaaatc atgatggaag
    32281 ccccccacgt tcaggcttgt gcgcaacaca ttacgcgctg gctttgcgca acctcaacgc
    32341 aaccggagaa cacgttatga tgccgtccgc ctccccaaaa caacgcgtac tgattgtggg
    32401 cgccaaattt ggcgaaatgt acctgaatgc ctttatgcag cccccggagg ggctggaact
    32461 ggtcggcctg ctggcgcagg gaagcgcccg ttcaagagag ctggctcatg cgtttggcat
    32521 tccgctgtat acctcgccgg aacagataac caggatgccg gatatcgcct gtattgtcgt
    32581 gcgttcgacc gtcgccggag gaaccggtac gcagcttgcc agacactttc tgacgcgcgg
    32641 cgtacacgtc attcaggagc atcccctcca cccggatgac atcagttcct tacaaacgct
    32701 ggcgcaggaa cagggctgct gctattgggt aaacacgttt tatccgcata cgcgcgcggg
    32761 acgcacatgg cttcgcgatg cgcagcagct gcgtcgctgc ctggctaaga cgccgccggt
    32821 agtccacgcc accaccagcc gacagttgct ctactccact ctggatttgt tgttgctggc
    32881 tctgggcgtt gatgccgccg ccgtggagtg cgacgtggtt ggcagcttta gcgattttca
    32941 ctgtctgaga ctcttctggc cggagggaga agcctgcctg ctgcttcagc gctatctcga
    33001 tcctgacgat ccagatatgc atagccttat catgcaccgc ctgctgctgg gctggccgga
    33061 aggtcatctt tcgctggagg cgagctacgg ccccgtcatc tggtcatcca gcctgtttgt
    33121 cgcagaccat caggagaacg cccacagcct ctaccgcaga ccggagatcc tgcgcgatct
    33181 gccgggtctg acgcgcagcg cggcgcccct gagctggcgc gactgctgcg aaaccgtcgg
    33241 gccggagggc gtcagttggt tgctacacca gttgcgcagc catctggcgg gcgaacatcc
    33301 accagcggcc tgccaaagcg tacatcagat cgcgttatca cgtttatggc agcagatttt
    33361 acgtaagacc ggcaatgcgg agatcagacg cctgacgccg ccgcaccacg accggttagc
    33421 cggtttctac aacgatgatg ataaggaggc gctgtgacgc aatctgcaat gtgcatcccg
    33481 ctgtggcccg cccggaacgg caatactgcg catctggtca tgtgcccttt cgctggcggc
    33541 agcagtagcg cgtttcgcca ctggcaagct gagcaactgg ctgattgcgc gctttctctg
    33601 gtgacctggc cggggcgcga tcgccttcgc catctggaac cgctcagaag cattacacaa
    33661 ctggcggcac tgctggcgaa cgagctggaa gcatccgtat cgcctgacac gccgctttta
    33721 ctcgccgggc acagcatggg ggcgcaggtg gcgtttgaaa cctgccgact tctggagcaa
    33781 cgggggcttg cgccacaagg actgattatt tccgggtgcc atgccccgca tctgcattct
    33841 gaacgccagc tcagccatcg cgatgatgcc gactttatcg ctgagctgat agacattggc
    33901 ggatgttctc ctgaactgcg ggaaaaccag gaattaatgt cgctgtttct tcctcttctg
    33961 cgcgctgatt tttacgccac cgagagctat cactacgact cgcccgacgt ctgtccgccg
    34021 ctgcgcacgc ctgcgctgtt attgtgcggc agccacgatc gcgaagcctc ctggcagcag
    34081 gtcgatgcct ggcgtcagtg gctgagccac gttacaggcc cggtggtgat tgacggcgat
    34141 catttctatc ccattcaaca agcccggtcc ttttttacgc agattgtccg ccattttccc
    34201 cacgcatttt ctgcaatgac cgcgttgcaa aaacagccca gtacttcaga aaggtgacgc
    34261 atgaattctt cctttgaatc tctgattgaa cagtatccct tacccattgc cgaacagttg
    34321 cgccactggg cggcccgtta tgcctcgcga attgccgtcg ttgatgcaaa ggggtcgtta
    34381 acctacagcg cgcttgatgc acaagttgac gaacttgccg caggtctgtc atcactgggt
    34441 ttgcgttcgg gggagcatgt aattgtgcag cttcccaacg acaacgcgtt tgttaccctg
    34501 ctgttcgcct tgttaagact gggcgttatc cccgtgctgg cgatgccctc gcaacgggct
    34561 ctggatatcg acgcgctgat tgagctggcg caacccgtcg cttacgttat tcacggggaa
    34621 aaccacgcag agctggcccg acagatggcg cacaaacacg cctgcttgcg tcatgttctg
    34681 gtcgctggag agaccgtgag cgacgatttt acgccgctct tctcccttca cggtgagcga
    34741 caggcatggc cgcagcctga tgtttccgcc accgcgttgt tgttgctctc aggcggcaca
    34801 accggcacgc ccaaactcat cccgcgccga catgccgact atagctataa cttcagcgct
    34861 tctgctgaac tgtgcggcat cagccaacag agcgtgtatc tcgccgtcct cccggtggcg
    34921 cataactttc cgctggcctg ccccggtatt ctgggaacgc ttgcctgcgg cggaaaagtg
    34981 gtgctgaccg acagcgccag ctgtgatgag gtgatgcctt taatcgcgca ggaaagagtg
    35041 actcacgtcg ccctggttcc ggcgctggcg caattatggg tgcaggccag ggagtgggaa
    35101 gacagcgacc tttcgtcgct gcgcgtcatt caggcaggcg gcgcccggct cgacccgacg
    35161 cttgctgagc aggttatcgc cacctttgac tgtaccctgc aacaggtttt cggtatggcg
    35221 gaaggcctgc tctgttttac ccggctggac gatccgcatg ccaccattct ccacagccag
    35281 gggcgcccgt tgtcccctct ggatgaaatc cgcatcgttg atcaagacga gaacgacgtc
    35341 gcgccgggcg aaaccgggca attgttaacg cgcggccctt ataccatttc gggctattac
    35401 cgcgctcctg cccacaacgc gcaggccttt accgcgcaag ggttttaccg cacaggcgac
    35461 aatgtcaggc tggatgaggt ggggaacctg cacgttgagg gacgcataaa agagcagatc
    35521 aaccgcgccg gagaaaaaat agccgcggct gaagtggaat cggcactgct gcgtttagcg
    35581 gaagtgcaag attgcgcggt ggtcgccgcg ccggacacgc tgcttggcga gcggatttgc
    35641 gcgtttatca tcgcgcagca ggtgccaact gactatcagc agttgcgtca acaactgacc
    35701 cgtatggggc tcagcgcgtg gaaaattcct gaccaaatcg agtttctgga ccactggccg
    35761 ctcaccgccg tcggcaagat agacaaaaaa cgcctgacgg ctctcgccgt cgaccgttat
    35821 cgccattctg cccaataagc gcaaaccgac ccgaaacagg ttgaaataaa cccgtttcgg
    35881 gtagcactac tattagaaat agttatcatt ttcaattcac cattgtcggt atttttggcg
    35941 tttcgccgtc ttacagggac tcacaacaat gaaaatgaca cggctttatc ctctggcctt
    36001 ggggggatta ttgctccccg ccattgctaa tgcccagact tcacagcaag acgaaagcac
    36061 gctggtggtt accgccagta aacaatcttc ccgctcggca tcagccaaca acgtctcgtc
    36121 tactgttgtc agcgcgccgg aattaagcga cgccggcgtc accgccagcg acaaactccc
    36181 cagagtcttg cccgggctca atattgaaaa tagcggcaac atgctttttt cgacgatctc
    36241 gctacgcggc gtctcttcag cgcaggactt ctataacccc gccgtcaccc tgtatgtcga
    36301 tggcgtccct cagctttcca ccaacaccat ccaggcgctt accgatgtgc aaagcgtgga
    36361 gttgctgcga ggcccacagg gaacgttata tggcaaaagc gctcagggcg ggatcatcaa
    36421 catcgtcacc cagcagccgg acagcacgcc gcgcggctat attgaaggcg gcgtcagtag
    36481 ccgcgacagt tatcgaagta agttcaacct gagcggcccc attcaggatg gcctgctgta
    36541 cggcagcgtc accctgttac gccaggttga tgacggcgac atgattaacc ccgcgacggg
    36601 aagcgatgac ttaggcggca cccgcgccag catagggaat gtgaaactgc gtctggcgcc
    36661 ggacgatcag ccctgggaaa tgggctttgc cgcctcacgc gaatgtaccc gcgccaccca
    36721 ggacgcctat gtgggatgga atgatattaa gggccgtaag ctgtcgatca gcgatggttc
    36781 accagacccg tacatgcggc gctgcactga cagccagacc ctgagtggga aatacaccac
    36841 cgatgactgg gttttcaacc tgatcagcgc ctggcagcag cagcattatt cgcgcacctt
    36901 cccttcaggt tcgttaatcg tcaatatgcc tcagcgctgg aatcaggatg tgcaggagct
    36961 gcgcgccgca accctgggcg atgcgcgtac cgttgatatg gtgtttgggc tgtaccggca
    37021 gaacacccgc gagaagttaa attcagccta cgacatgccg acaatgcctt atttaagcag
    37081 taccggctat accaccgctg aaacgctggc cgcatacagt gacctgacct ggcatttaac
    37141 cgatcgtttt gatatcggcg gcggcgtgcg cttctcgcat gataaatcca gtacacaata
    37201 tcacggcagc atgctcggca acccgtttgg cgaccagggt aagagcaatg acgatcaggt
    37261 gctcgggcag ctatccgcag gctatatgct gaccgatgac tggagagtgt atacccgtgt
    37321 agcccaggga tataaacctt ccgggtacaa catcgtgcct actgcgggtc ttgatgccaa
    37381 accgttcgtc gccgagaaat ccatcaacta tgaacttggc acccgctacg aaaccgctga
    37441 cgtcacgctg caagctgcga cgttttatac ccacaccaaa gacatgcagc tttactctgg
    37501 cccggtcggg atgcagacat taagcaatgc gggtaaagcc gacgccaccg gcgttgagct
    37561 tgaagcgaag tggcggtttg cgccaggctg gtcatgggat atcaatggca acgtgatccg
    37621 ttccgaattc accaatgaca gtgagttgta tcacgataac cgggtgccgt tcgtaccacg
    37681 ttatggcgcg ggaagcagcg tgaacggcgt gattgatacg cgctatggcg cactgatgcc
    37741 ccgactggcg gttaatctgg tcgggccgca ttatttcgat ggcgacaacc agttgcggca
    37801 aggcacctat gccaccctgg acagcagcct gggctggcag gcgactgaac ggatgaacat
    37861 ttccgtctat gtcgataacc tgttcgaccg tcgttaccgt acctatggct acatgaacgg
    37921 cagcagcgcc gtcgcgcagg tcaatatggg tcgcaccgtc ggtatcaata cgcgaattga
    37981 tttcttctga ttattgtaaa agggataccg aaaaggtatc ccttttacac cactagttaa
    38041 aaccagtaac tcagcagagt cgcaaaaaat attaatccat agtgattatt taaacaatga
    38101 aattgcgatt aggataaata gatttaactt tctcgttcct ttctctcctt atactaaaga
    38161 aataatcata tcaaaataaa aattcacaac agtgcaacat taaaaaatac aaccaacaaa
    38221 caatcctata tacaaggcac atctccagaa tataaaagca cagacaaaca acctaaaaaa
    38281 aacaacccca tacatataaa ttattttata ggtaaaatag attatatatt ctcatagcta
    38341 ccacaaacta tactagcctg aactatattt attctgctgc aatcaatgca tacataacac
    38401 aaatatcact caggtacact actcaaacca cgctgggatt tttcctcaag ttataatcat
    38461 caccccgaaa atcattcggc atttactcat taaatagtca ccccataggc ctgtacatgt
    38521 ttactcagaa atatacatcc ttttctctgt cataaaccct ctgattaatc ataaataaat
    38581 acttgtgaca ccaatctttt tccttaacgg aacgaattgt tgtgtagaag gagataatat
    38641 tatgtcaaaa aaaatatcag ccattgctta taactcatta tatgtcaaca tgggtcacta
    38701 taacggaagc agttgagatc actaccaaag ccataaaaca aaaaattact cctagcgata
    38761 tttatcgtca tgccttgagt ggcaacatcc tactatcggt ctatttccag tctcctgtga
    38821 tacttaagaa gatacaaact tttaatggaa aaataaaatt caggcagttt gtgggagacc
    38881 tacttgataa actatgcatg cttgacaggg acggttttat ttatggtcaa aatttaagac
    38941 tctgtacaga agctagatat atttgtcctg tgcaacaaat tatagatacc cccctgttac
    39001 gcaaactcaa ccagtttcgt acgtttgttc gtaatgtccg cccgggtgat gaactggatg
    39061 tcccggcaca agttagtgaa aaaaatttaa ccccgccgcc gggtaacagc agtggcaacc
    39121 ttgagcaaca gatagccagt acttcacagc taattggatc tctgctcgcc gaagatatga
    39181 acagcgagca agcggcaaac atagcacgtg gatgggcctc ttctcaggct tcaggggtaa
    39241 tgacagactg gttaagccgc ttcggtaccg caagaatcac gctgggcgtg gatgaagatt
    39301 tcagcctgaa gaactcccgt gacggcaacc ctcgaaaatg gcgattcgat gcaacaaaca
    39361 gtgaactatg tgccgaacgt cacgaatgct gaaatcacgc tggcagcctc gaaggacccg
    39421 gtgattgccg acaataacga tctcacgaca ctaacagcac cgtcgctgat acagagggca
    39481 atgcgatagc caacactgag gtaacattta ctctaccgga agatgtgaag gcgaacttca
    39541 cgctgagcga tgacgacaaa gcggttactg atgccgatgg caaagcgaaa gtcacgctgt
    39601 aaggtacaaa agcaggcgct cataccgtga cagcatcgat ggttggcggt aagagtgagc
    39661 agttggtggt gaactttact gcggatacgc tcactgcgca ggttaatctt aacgttaccg
    39721 aggacaattt catcgccaat aacattggaa tgaccaggct acaggcaaca gtgactgatg
    39781 gaaacggcaa cccagtagaa ggtataaaag ttaatttccg tggaacctcc gtcacgctaa
    39841 gcagcaccag cgttgaaacg gatgatcagg ttttcgctga aattcttgtg acaagcaccg
    39901 aggtcggact aaaaacagtt tcagcctctc tggcagataa acctactgaa gtcatctcgc
    39961 gattactgaa tgccaaggta gatgttaatt ctgcgacgat taccagtcag gagatacctg
    40021 aaggtcaggt catggtcgca caagacatag cagttaaagc tcacgttaac gaccagtttg
    40081 gcaacccggt gacgcatcaa cccgcgacat tcagtgcagc gccatcctcg caaatgatca
    40141 tcagccagaa tacggtctct actaatacgc agggagtagc cgaggtcacc atgacgcctg
    40201 aaagaaacgg ctcgtatact gtaaaagcat ccctagcgaa tggagcttca cttgagaaac
    40261 aactggaggc tattgatgaa aaactgacac tcacgtccag tccgcttatc ggtgtcaatg
    40321 ctcctaaagg cgctactctg acggcaatgc taacctctgc aaatggcact ccagtggagg
    40381 gtcaggtcat caactttagc gtaacgctag aaggtgcgac gttaagtggc ggaaaagtga
    40441 gaaccaactc ttcaggtcag gctccagtcg ttctgaccag caataaagtc ggtacatata
    40501 cggtgactgc atcgttccat aacggcgtca caatacagac acagacaacc gtgaaggtta
    40561 ctggcaaccc aagcaccgca catgttgcta gctttatcgc tgaaccgtcg actatcgccg
    40621 ccaccaacag tgatttaagt accttaaagg caacggttga ggatggcagt ggtaacctga
    40681 tcgaaggtct cactgtgtac ttcgccttaa aaaacggctc caccacatta acgtcattaa
    40741 cagcggtgac cgatcaaaac ggaatcgcga caacaagcgt gaaaggagcg ataacgggga
    40801 gcgtcactgt aagcacagtc acaagcgcag gtggaatgca aacagtagat atatcgctag
    40861 tggcaggccc agcagacgcc tcgcagtcca tccttaagaa caaccagtca tcactgaaag
    40921 gggactttac cgatagtgct gagctacatc ttgttctgca cgatatatca ggcaatccga
    40981 tcaaagtttc tgaagggatg gaatttgtgc aatcaggtac aaacgtgccc tatatgaaaa
    41041 ttagcgcaat tgattacact caaaatatca acggtgatta caaagctact gttacaggag
    41101 gcggagaggg tatcgcaacg ctgatccccg tattgaatgg tgttcatcag gcgggcctga
    41161 gtaccacgat agaattcatt agcgcggaaa ccagaccaat gaccggtaca gtgtcagtga
    41221 atggtgcaaa cctaccgaca gcttcattcc cttcgcaggg atttaccggg gcgtattatc
    41281 agttgaataa tgacaacttt gcaccaggaa aaacggcggc tgattattcg tttcaaactc
    41341 ggcctcttgg gtaggcgttg atgctactgg taaagtgaca ttcaaaaatg acggtgatag
    41401 caatactgta ataatcacag cgcccccaag atccggtggt gctatttacc aaacagttcc
    41461 gcatgaatcg cgttcggtgt gactatgatc attttcggtg agtattgctt tgatccaaag
    41521 gctggttttc tgtgatgatt gaccagcctc agagtattga tgacaccacc gttcataaca
    41581 gatcggcatt actatgcata agtactgcct caaattctcg ccaaaacact ttttaatgcc
    41641 tttgcgaatt catccagact tatattgtca attttttgcc caacgatgac tgactccaaa
    41701 acatcggcca ttgcgggcca tcgcgttttg taatcagcat aaattgcaac aacaggggta
    41761 ttataggcac ttgccatatg taatattgag gtgtcaggag ttattgcaat ataagcatct
    41821 tttatgattg ctgctgaatg tttaatagag ggtgataagg ataaacggta gacattatta
    41881 taacaatcca ctaatgccct ggctttatct tcccctttgg gtccataaac gatcactatt
    41941 ggaatatctg tttcactttg aatttttgct attatatttt ctgcaatcga aaatgaaaat
    42001 atacgttcct gcgagctacc ttctaaattg agcgcaatgt aagatcctaa cgatcgcatt
    42061 tcctcgcgta cctcatcgag tacatcatcg cttaatggca attcatatat tgacctgact
    42121 gccggaaaac ccgcctcacg catcataaat gcccatgtca tgggtacagg agcccggaga
    42181 ttctgatcca tccgggacgc gttcttacac aaaggggaat agcacttcat ggttaaaccg
    42241 acgacctgga aattcgtttt tgctttcaac tgactgataa atatcatcgt tttcaggttc
    42301 tttttacgca tcgcctctat acaaagatcc ggcgtaccgt attgcagtgt tatgttcttc
    42361 gctaaatatt ttatttcttt taatgttgcg tgatcctgca tagtcattgt gactaatgtt
    42421 aatttagtct gttcaagttt gagcgcatta aagacttcta aattaattgt cgacgtaaca
    42481 attaatagat gcttaatttt atgcaattca agcgcccgaa taacaggaaa gatggccata
    42541 gcatcgccaa tctgatcggg aatatgtatg acaacaaagt ctgttttttc aatattaaaa
    42601 ttataagctt tataatcgta gtaactaaat gcaatacgtc tcaacaatga tactaaaaac
    42661 ataactaacc tcgcctccct actggttata atgcaatgca gtctatcaga atcatcaggg
    42721 tacaattttg tgcatatgcg gacttttatg tttcatatct cgaacctgtg ggccctctgc
    42781 ttaatcctta aacaacacca gcaactcctg cgctttcatc ttccatcgaa tttttcatgt
    42841 tgccgctaat cagccataaa atcatttgca gatgcgctct gtcgaggtag tctcataagg
    42901 ttcgtttata gatcgacggc aatgtgagtt accttttcca tactaattat aaaaagacag
    42961 tacaaacagg atcattatgg actccacgct catctccact cgtcccgatg aagggacgct
    43021 ttcgttaagt cgcgcccgac gagctgcgtt aggcagcttc gctggtgccg tcgtcgactg
    43081 gtatgatttt ttactctatg gcatcaccgc cgcactggtg tttaatcgcg agtttttccc
    43141 gcaagtaagc ccggcgatgg gaacgctcgc cgcatttgct acctttggcg tcggattcct
    43201 tttccgtccg ctcggcggtg tcattttcgg tcactttggc gaccgactgg gacgtaagcg
    43261 catgttaatg ctgaccgtct ggatgatggg catcgcgaca gccttgattg gtattcttcc
    43321 ttcattctcg accattgggt ggtgggcacc tattttgctg gtgacactgc gtgccattca
    43381 gggatttgcc gtcggcggcg aatggggagg cgcggcgttg ctttccgttg aaagtgcacc
    43441 gaaaaataaa aaagcctttt acagtagcgg tgtacaagtt ggctacggtg taggtttact
    43501 gctttcaacc ggactggttt cattgatcag tatgatgacg actgacgaac agtttttaag
    43561 ctggggctgg cgcattcctt tcctatttag catcgtactg gtactgggag cattgtgggt
    43621 gcgcaatggc atggaagagt ccgcggaatt tgaacaacag caacattatc aagctgccgc
    43681 gaaaaaacgc atcccggtta tcgaagcgct gttacgacat cccggtgctt tcctgaagat
    43741 tattgcgcta cgactgtgcg aattgctgac gatgtacatc gttactgcct ttgcacttaa
    43801 ttattcaacc cagaatatgg ggctaccgcg cgaacttttc cttaatattg gtttgctggt
    43861 aggtggatta agctgcctga caattccctg ttttgcctgg cttgccgatc gttttggtcg
    43921 ccgtagggtt tatatcacag gtgcgttaat cggaacgttg agcgcatttc ctttctttat
    43981 ggcgcttgaa gcacaatcta ttttctggat agttttcttc tccataatgc tggcaaacat
    44041 tgcgcatgac atggtggtgt gtgtgcaaca accgatgttt accgaaatgt ttggtgccag
    44101 ttatcgctat agtggcgctg gagtcggtta tcaggttgcc agtgtggttg gcggtggatt
    44161 tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc atagcgtcgc
    44221 catttatttg ctggctggat gcctgatttc cgcaatgacc gctttgttga tgaaagacag
    44281 tcaacgcgct tgatagcctg gcgaagatca tccgatcttc gccttacact tttgtttcac
    44341 atttctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac acacatgaat
    44401 aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc gctgtatgag
    44461 caatctgtag tcgcattacg caacgccatt ggcaactata ttacaagtgg cgaattacct
    44521 gatgaaaacg cccgcaaaca aggtcttttt gtctatccat cactgaccgt aacctgggac
    44581 ggtagcacaa ccaatccccc caaaacgcgc gcatttggtc gttttactca cgcaggcagc
    44641 tacaccacca cgattactcg ccctactctc tttcgttcgt atcttaatga acaacttacg
    44701 ttgctgtatc aggattatgg tgcgcatatc tctgtgcaac cctcgcagca tgaaatccct
    44761 tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag cgctgggtta
    44821 actcgctact tcccgacaac agaactggcg caaattggcg atgaaactgc agacggcatt
    44881 tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt cgatttttcc
    44941 ctcgcacggt tgcgccatta taccggtacg ccagttgaac attttcagcc gttcgtcttg
    45001 tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag ccagatcctc
    45061 gatcctgata gtccttacat tgccctttct tgtgctggcg ggaactggat caccgccgaa
    45121 accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca gatgccagca
    45181 tggcatttaa ttaccgccga tgctcagggt attactctgg tgaatattgg cgtgggaccg
    45241 tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatgt ctggttgatg
    45301 attggtcact gtggcggatt acgtgaaagt caggccattg gcgattatgt acttgcacac
    45361 gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat tcctattccg
    45421 agcattgctg aagtgcaacg tgcgctttat gacgccacca agctggtgag tggcaggccc
    45481 ggtgaggaag tcaaacagcg gctacgtaca ggtactgtgg taaccacaga tgacaggaac
    45541 tgggaattac gttactcagc ttctgcactt cgttttaact taagccgggc cgtagcaatt
    45601 gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc atacgggaca
    45661 ctactgtgtg tttcagataa accgttgcat ggcgagatta aacttcccgg tcaggctaac
    45721 cgtttttatg aaggtgctat ttccgaacac ctgcaaattg gcattcgggc gatcgatttg
    45781 ctgcgcgcag aaggcgaccg actgcattcg cgtaaattac gaacctttaa tgagccgccg
    45841 ttccgataat aagttctgaa ccttctcatc agaaatagat gtgaaggatt aagcaagaca
    45901 tctggcaaat agcctgcaat caacgcgggc tgtttgcttc cttgggcaga tgcgagtttc
    45961 attatcgtct taatgatttc cgcatattaa aagcatgtat gctttcaaaa tataattata
    46021 aaaaatcccg ccaacaacat tagtttttat aaaacaaaat ataagattat ggctttagaa
    46081 tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgct gatttaacac
    46141 atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc agtgggacgt
    46201 aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc taaaatttat
    46261 gcaaaattcg gtgtagaaat ctatgctgct gctaaacaag gtgaacccga tccagaatta
    46321 aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc aaagcacgtt
    46381 attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt gcagggccgt
    46441 tatgaaggct ttggtcctaa tggctcaatg attatcgccg agacattgac ttcaaatgtt
    46501 aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa tatcggagcg
    46561 gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa aggatcagac
    46621 cctgaccata tttttgaaat tttacttgaa gctgaagttg atgttcgtga tgtgactgaa
    46681 gaagaaggta acattgttat ttataccgaa cctactgacc ttcataaagg aatcgcggct
    46741 ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat tgcgcaatct
    46801 gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga tgcccttgaa
    46861 gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctcta
//
LOCUS       DAPOZO010000031        46223 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPOZO010000031 DAPOZO010000000
VERSION     DAPOZO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.031"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(234..1604)
                     /gene="cpsG"
                     /locus_tag="ROI21_001885"
                     /gene_synonym="manB"
     CDS             complement(234..1604)
                     /gene="cpsG"
                     /locus_tag="ROI21_001885"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDX5110806.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(1797..3233)
                     /gene="cpsB"
                     /locus_tag="ROI21_001886"
     CDS             complement(1797..3233)
                     /gene="cpsB"
                     /locus_tag="ROI21_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDX5110807.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(3236..4459)
                     /gene="wcaI"
                     /locus_tag="ROI21_001887"
     CDS             complement(3236..4459)
                     /gene="wcaI"
                     /locus_tag="ROI21_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDX5110808.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVNAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(4456..4935)
                     /gene="gmm"
                     /locus_tag="ROI21_001888"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(4456..4935)
                     /gene="gmm"
                     /locus_tag="ROI21_001888"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDX5110809.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFVRLTMAELGLRLPITSGKFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(4935..5903)
                     /gene="fcl"
                     /locus_tag="ROI21_001889"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(4935..5903)
                     /gene="fcl"
                     /locus_tag="ROI21_001889"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDX5110810.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDANKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(5906..7027)
                     /gene="gmd"
                     /locus_tag="ROI21_001890"
     CDS             complement(5906..7027)
                     /gene="gmd"
                     /locus_tag="ROI21_001890"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDX5110811.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(7053..7601)
                     /gene="wcaF"
                     /locus_tag="ROI21_001891"
     CDS             complement(7053..7601)
                     /gene="wcaF"
                     /locus_tag="ROI21_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDX5110812.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGYGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(7617..8363)
                     /gene="wcaE"
                     /locus_tag="ROI21_001892"
     CDS             complement(7617..8363)
                     /gene="wcaE"
                     /locus_tag="ROI21_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDX5110813.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(8374..9591)
                     /gene="wcaD"
                     /locus_tag="ROI21_001893"
     CDS             complement(8374..9591)
                     /gene="wcaD"
                     /locus_tag="ROI21_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDX5110814.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(9566..10783)
                     /gene="wcaC"
                     /locus_tag="ROI21_001894"
     CDS             complement(9566..10783)
                     /gene="wcaC"
                     /locus_tag="ROI21_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDX5110815.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHLHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVRQMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(10780..11268)
                     /gene="wcaB"
                     /locus_tag="ROI21_001895"
     CDS             complement(10780..11268)
                     /gene="wcaB"
                     /locus_tag="ROI21_001895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDX5110816.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(11271..12110)
                     /gene="wcaA"
                     /locus_tag="ROI21_001896"
     CDS             complement(11271..12110)
                     /gene="wcaA"
                     /locus_tag="ROI21_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDX5110817.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(12203..14365)
                     /gene="wzc"
                     /locus_tag="ROI21_001897"
     CDS             complement(12203..14365)
                     /gene="wzc"
                     /locus_tag="ROI21_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDX5110818.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEDKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(14368..14811)
                     /gene="wzb"
                     /locus_tag="ROI21_001898"
     CDS             complement(14368..14811)
                     /gene="wzb"
                     /locus_tag="ROI21_001898"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDX5110819.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(14817..15956)
                     /gene="wza"
                     /locus_tag="ROI21_001899"
     CDS             complement(14817..15956)
                     /gene="wza"
                     /locus_tag="ROI21_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDX5110820.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            16615..18198
                     /locus_tag="ROI21_001900"
     CDS             16615..18198
                     /locus_tag="ROI21_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDX5110821.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RVVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(18472..20325)
                     /gene="asmA"
                     /locus_tag="ROI21_001901"
     CDS             complement(18472..20325)
                     /gene="asmA"
                     /locus_tag="ROI21_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDX5110822.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGVNFTDVATQMTNKSGLLEIIQLQGKLNGGQVSLPGTLD
                     ATSVNPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(20347..20928)
                     /gene="dcd"
                     /locus_tag="ROI21_001902"
     CDS             complement(20347..20928)
                     /gene="dcd"
                     /locus_tag="ROI21_001902"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDX5110823.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(21020..21661)
                     /gene="udk"
                     /locus_tag="ROI21_001903"
     CDS             complement(21020..21661)
                     /gene="udk"
                     /locus_tag="ROI21_001903"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDX5110824.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            21979..25296
                     /locus_tag="ROI21_001904"
     CDS             21979..25296
                     /locus_tag="ROI21_001904"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDX5110825.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     THDALTHLANRASFEKQLRILLQTVNNTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAATHSERAA
                     ISLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELIEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(25334..26191)
                     /gene="alkA"
                     /locus_tag="ROI21_001905"
     CDS             complement(25334..26191)
                     /gene="alkA"
                     /locus_tag="ROI21_001905"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDX5110826.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADDYYARSLAVGEC
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLSMTIPGDVEQAMKMLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            26325..27677
                     /gene="yegD"
                     /locus_tag="ROI21_001906"
     CDS             26325..27677
                     /gene="yegD"
                     /locus_tag="ROI21_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDX5110827.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALNQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(27690..29630)
                     /gene="yegI"
                     /locus_tag="ROI21_001907"
     CDS             complement(27690..29630)
                     /gene="yegI"
                     /locus_tag="ROI21_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDX5110828.1"
                     /translation="MKKNIKVFTSTDELTTLGRELGKGGEGAVYDIEEFVDSVAKIYH
                     TPPPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMI
                     YSPAHRRQRYPHCAWDFLLYVARNIASSFATVHEHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQAAGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALQDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            29828..30289
                     /locus_tag="ROI21_001908"
     CDS             29828..30289
                     /locus_tag="ROI21_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegJ family protein"
                     /protein_id="HDX5110829.1"
                     /translation="MKKILLMLSLLFFTTAGFSEVSDTLVTGGYDKQAMSDAIQHARK
                     ETDKFIEVMNKKDADTFAVKAPITDHGRTEHFWLTDVTYSNGMFIGVISNDPGIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
     gene            complement(30354..31115)
                     /gene="pphC"
                     /locus_tag="ROI21_001909"
     CDS             complement(30354..31115)
                     /gene="pphC"
                     /locus_tag="ROI21_001909"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HDX5110830.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELEVRDFACTFLGLISSPDGTLIMQIGDGGMVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSGPAVNERTDDDKTLALALWTE"
     gene            complement(31112..31771)
                     /locus_tag="ROI21_001910"
     CDS             complement(31112..31771)
                     /locus_tag="ROI21_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDX5110831.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSHSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(31992..32051)
                     /gene="ibsA"
                     /locus_tag="ROI21_001911"
     CDS             complement(31992..32051)
                     /gene="ibsA"
                     /locus_tag="ROI21_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDX5110832.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(32324..32380)
                     /locus_tag="ROI21_001912"
     CDS             complement(32324..32380)
                     /locus_tag="ROI21_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDX5110833.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(32652..32708)
                     /locus_tag="ROI21_001913"
     CDS             complement(32652..32708)
                     /locus_tag="ROI21_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDX5110834.1"
                     /translation="MMKWIIIVVLLVISYPAY"
     gene            32987..34234
                     /gene="mdtA"
                     /locus_tag="ROI21_001914"
     CDS             32987..34234
                     /gene="mdtA"
                     /locus_tag="ROI21_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDX5110835.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKTGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            34234..37356
                     /gene="mdtB"
                     /locus_tag="ROI21_001915"
     CDS             34234..37356
                     /gene="mdtB"
                     /locus_tag="ROI21_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDX5110836.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            37357..40434
                     /gene="mdtC"
                     /locus_tag="ROI21_001916"
     CDS             37357..40434
                     /gene="mdtC"
                     /locus_tag="ROI21_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDX5110837.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     VLEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            40435..41850
                     /locus_tag="ROI21_001917"
     CDS             40435..41850
                     /locus_tag="ROI21_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5110838.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LRMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTVPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAITGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLIATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            41847..43250
                     /gene="baeS"
                     /locus_tag="ROI21_001918"
     CDS             41847..43250
                     /gene="baeS"
                     /locus_tag="ROI21_001918"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDX5110839.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAGQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            43247..43969
                     /gene="baeR"
                     /locus_tag="ROI21_001919"
     CDS             43247..43969
                     /gene="baeR"
                     /locus_tag="ROI21_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDX5110840.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            44149..44481
                     /locus_tag="ROI21_001920"
     CDS             44149..44481
                     /locus_tag="ROI21_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDX5110841.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            44629..45990
                     /gene="yegQ"
                     /locus_tag="ROI21_001921"
     CDS             44629..45990
                     /gene="yegQ"
                     /locus_tag="ROI21_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDX5110842.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTSERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWIPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
BASE COUNT        10687 a        12373 c        11999 g        11164 t
ORIGIN      
        1 tagcccggca aacatgatgg tgatatctga aaagcgttgc accatagaga ttaacgatgc
       61 attggttttc gctcgctcgc gcttttttag atttgtcatc gttgttcctg ttctattgtc
      121 gcctcgcccc tcaccccggc cctctcccaa aggggcgagg gggagaagaa agttccgtgc
      181 tcacttctct ccgcaggcag tagagcgtca gggtgagggg gaaagtaatg acattactcg
      241 ttcagcaacg tcagcagagt tcgcgttcgc gcttccatca gcggtacatc accgcgcgat
      301 tccacattca accgcaccac cggttcggtg ttggaggaac gcaggttaaa gcgccagtcg
      361 gcaaaggtca tgctgatacc atcggtgcga tccactgcca gcgcctcacg gctaaagtgc
      421 tgttccacgc ggttaatcgc ctcaacgggt tgcgccagtt tgctgttgat ctcaccgctt
      481 gccggaaacg ccgccatccg gtcgcgtacc agttcgccca gcgtttttcc tttcaggcac
      541 accagttcgg cgaccagcag ccatgggatc atgccgctgt cgcagtaagc gaaatcacgg
      601 aagtaatggt gggcgctcat ttcgccaccg tagatggcgt cttccttgcg catccgttct
      661 ttaataaagg cgtgtccggt tttcgacatt accggcgtgc cgcctgcagc agtcaccaca
      721 tcaatagtgt tccaggagag acgcggatca tggatgatct tcgcgccggg atttttttcg
      781 aggaacgctt ctgccagcag gccgacaatg tagtagccct cgataaactg ccctttttcg
      841 tcaaacagaa aacagcggtc aaaatcgcca tcaaaggcaa tgcccatatc cgcgccgtgt
      901 ttgatgaccg cattgcgggt gtcgtcgcga cattccggta gcagcgggtt aggaataccg
      961 ttggggaaat tgccgtccgg tgtgttgtgt actttgatta attccacggg cgcgccgagg
     1021 gctttaaagc gggcttcaat ggcgtccacc accggacccg ctgcgccatt cccggagttg
     1081 atcaccagct tgagcggcgt gagatttttg acattgatat aaccgaacag gtgatcaacg
     1141 taagcgtcac gcaggttgat ttgctgatag cgaccgcgtt tggtttcatc gacgggagga
     1201 aagtcgttgg cttcagccag acgctggacg tcgcgcagtc cggtatctcc gctgatcggg
     1261 cgagccccct cgcgaaccag cttcatgccg ttataatcca tcggattatg gctggcggta
     1321 acttcaatgc cgccatccac gccgagatgg aacgtggcga aatagatctc ttcggtgccg
     1381 gacataccaa tatccagcac gtcaacgccc gcatcctgta aaccttttgc cagcgccagt
     1441 tttaaggttt cgctggtgag acggacatca ccgcctaaca caatggtttt cggtttgaga
     1501 aattcgccat aggcgcgacc aatgcgccag gcgatatctt cattcagttc ttcgcctaat
     1561 ttcccgcgaa tatcataggc tttaaagcag gttaattttt tcatgtcgtt accctttttc
     1621 aggcaatagt tggccctgac cgaaacggcc aattttgttt ttgtcgttat ttacatctcg
     1681 gtttcgcatt cattgcctga tgcgacgctg ccgcgtctta tcaggactac aaacccgaac
     1741 cgtaggtcgg ataaggcgtt cacgccgcat ccgacaaaca gctcctgatg cgacgtttac
     1801 acccgtccgt agcgatccgc gaaacgcacc acatcatcct cttcgagata agagccggag
     1861 cgcacttcaa ttaaatcgag cggaattttc cccgggtttt ccaggcaatg cgtcgccccc
     1921 agcggaatat aaatggactc gttttcacca agcagtttga tatcaccatc aatggtgact
     1981 tttgccgttc ccgcgacaac cacccagtgt tccgcgcggt gatggtgcat ctgtaccgac
     2041 aagccctcgc ccggtttcac ggtgatgcgt ttcacctggt agcggtcgcc cgcgtcgata
     2101 gagtcatatt tgccccacgg gcgatagact tcgcgatgca cccgatgctc atggcgacca
     2161 tcggctttga tctgctcgac cacttttttc acatcctgca ccgcgttacg gtcggcaatc
     2221 agcaccgcat ctttggtctg caccactacc agatctttca ccccgacggt ggttacgagg
     2281 ccagattcgg cgtacacata gctgttttca gttttgtgat taatcacatc gccgtggcaa
     2341 acgttgccct cggcggtgtg ggcgctaatt tcccataatg aggaccagga gccgacatcg
     2401 ctccagcccg catccatcgg caccacaacg gcatctgccg tacgttccat gaccgcgtaa
     2461 tccaccgact cttccgggca ggcgagaaac gcttcttcat ccacacgaat aaaatcgaga
     2521 tccggatcga cggcgctcat cgctttttca caggcatcga gaatatccgg acgatatttt
     2581 ttcagttctt cgagatagcg tccggcgcgg aacaggaaca tgccgctgtt ccagtaatat
     2641 tcgccgcttg ccacataggc ctgggcggtt tccagattcg gtttttcgac aaactgcgcc
     2701 acttcaaagg ccaccgtatc ctgctcaccc gcagacactt caccgcgacg aatatagcca
     2761 taaccggttt ctggtagatc cggcacaatg ccgaaggtca ccagcttgcc cgcttcggca
     2821 tatggcatgg cattacgcac ggcggcacgg aacgcgtctt catcggcaat cacatgatcc
     2881 gccgccaata ccagcattaa cgggtcgctc tccgggctat gacgttttgc cgccagcgcc
     2941 gccagcgcaa tggcaggtgc cgtgtttcgc cctgccggtt cgagaataat gttctcggta
     3001 agtttgttca gttgacgcag ctgttccgcg acaataaagc ggtgctgctc attgcaaatc
     3061 accaccgggc tttcgcactc cacgccgttc aggcggcaga tggtggtttg cagcatggtg
     3121 agatcgcctt tcaggcataa aaactgcttg gggtaaagta cgcgggaaag cggccataag
     3181 cggctaccgg agccacctgc catcacaact ggatagagtt tcgactgcgc cataattatc
     3241 cccgaatatc atttataaat tgacgtaaca cgttctcttt atcgagtgtg cgttcggcat
     3301 attcacgtgc caccgtgttg tgtttgggca gcaggagcgc ctgacggatc cccgccacca
     3361 gcgcctcgac cgattccggt tctacgcaaa ccgcaatgcc cggaaaggtt tcgcaaagct
     3421 gccccagttc tgtgtgggcc tctgcggtaa tcaccgcgtt accgcctacc gccagaatat
     3481 tggtcagttt cgacggcaat acggcatctg ccgcgccgcg tttttgcacc accagatggc
     3541 aatcgcccat cttcagcagt gcgggtaaag cgtcatacga ttgcagcggg aaaaattgca
     3601 tgttgcgcag tccacgctgc tgcgccattt tttccagccg cgctttgccg ccgccctgcc
     3661 cgacaatggc aaaaatcagc ggttcatcgc gcagacgatc ggcagcttca ataacgtttt
     3721 ccagcccctg cttttcacca atattgccgg agtaaagaat gatttttttg ttatccggca
     3781 ggtcaagctg gttacgaagg gcgtcaacat cggcatctgc aacatgctga aaacgggcaa
     3841 tttccgacca gttggggaag aagatgacgt tttccgccgc cacgcctttt tcgatggctt
     3901 tattcatcat cgaacgcgaa atcgtggaga cgttatcgac gttatgcagt ccgctacgtt
     3961 cgaacgccgt tgccagctgt gccactttgc cgcctttgcc ttttccggca aggcccagcc
     4021 ccagcatggc gttcacttcg taatcctgaa tatgcagcac ggtacgcgca ccagagagtt
     4081 tcgccagcag acgcattccc ggcgtgcaaa acagcgttgg caccacgccg ataatgcgat
     4141 ccggcttcca gcgacgttgc gccatcaacg ggaaaaaact gctgacggca aaactgccga
     4201 gatgcaacag gcgtttcagg gtgctcggct gttttggcac gtacagcggg cagcgccaca
     4261 ccgtggcggc cccctcttct cgtttgtagc gccaggcgga atagttctcg cccacctgcc
     4321 actgcgggta gtaaggcggt gcggtaatga cccgcacctc atgaccttgt gccgccagcc
     4381 attccaccat ctcgccggtg tatttgccga tgccggttaa ctccggcgag tagttaatgc
     4441 cgtagaccag tattttcata atccgggtac tccggcacgc ttctcagcga gaaaataggc
     4501 gcggctgtta gcgtgaacat tgtcactggc gagcagtgcg tccggcgtca gccagcggta
     4561 atcgtcatgc tgttcatccg gcagtaacag ctcttcttca gcgactctga agcgaaaacc
     4621 gagcaccaca tagtgagtgg tgaaatccgt gccggagaag ttatcgtcat aaaagtgctg
     4681 ccagacaccg taaaacttgc ctgatgttat cggcagacgc agccccagtt ccgccatcgt
     4741 cagccgcaca aatgcggctt ccagcgtttc gtctttctgc acgcgccctc ccggcacaaa
     4801 ccagtaaccc tgcgccgggc ggttggttct tttgccaagc agaaactcgc cgcgactgtt
     4861 ctcgacaata aagtcgagag agacaagcgg tgtggagcgc actacagtgg caaagtcttc
     4921 ctgacgtaaa aacatcattc cccccgaaag cggtcttgat tctcaaggaa ccactggtaa
     4981 gtgctggcaa gccccgcttc cagtgagatt tcgtgatacc agccaagctg atgcaggcgc
     5041 gtcacatcca gcagtttgcg cggcgtacca tccggtttgc tggcatcaaa aaccacccga
     5101 cctttgtaac ccaccacttt ggcgatggtt tgcgccagct cgcggatagt gcagtcaacg
     5161 cccgtaccaa cgttaatgtg cgacagcatc ggctgggtgt tctccagcca gacttcatgc
     5221 gccagctcca tgacatgaat gctcgccgcc gccatgtcat cgacgtgcag gaattcacgc
     5281 atcggtgtac cgctgcccca taccaccacg tccggcgcat tctgtgccgt cgcctcgtgg
     5341 aagcgacgca gcaatgctgg gatcacatgc gagttactcg ggtggaagtt gtcatgcggc
     5401 ccgtacaggt tggtcggcat gacagagcgg taatcgcgtc catactggcg gttgtatgat
     5461 tcgcacagtt tgatcccggc gattttggca atggcgtaag gctcgttggt cggctccagc
     5521 gtgccctgca ataactcgct ttctgccatc ggctgttttg ccagtttcgg gtagatgcag
     5581 gacgatccga gaaacagcag tttgttcgcg tcgttctgat gcgcggcgtg aatgatgttg
     5641 ctctcaatca tcatgttctg gtagatgaaa tccgccggat aggtgttgtt ggcaacaatg
     5701 ccgcccactt tcgccgccgc cagatagacc tggtcaatgc gttcgctggc aaagaaatca
     5761 tgcacggcgc ggctgtccag caggttcagc tcgtcgcggg tacgtaatac cagctccaca
     5821 tcaccgcgct gttcgagctg ccgcttgatg gcagaaccga ccatcccacg atggccagcg
     5881 ataaaaatgc gttgtttact catgcttatg actccagcgc gatcgccacg tcgtagccgt
     5941 gagatttcag cagagagtgt tttttcgccg cttcgaggtc attagccacc atttcagaca
     6001 ccatctctct gagggtgatt tccggtttcc agcccagttt ttcgtgcgct ttggtcgggt
     6061 cgccgagcag cgtttcaact tccgccggac ggaagtaacg cgggtcaacg gcgataatca
     6121 catcgcccgg tttaacgccc ggcgcgtcat gcccggtgac ggaaaccaca atgcccttct
     6181 cttcaacgcc cgtgccttca aagcgcagtt tgatgcccag ctgtgctgcc gccatttcca
     6241 cgaactgacg tacggagtac tgaacgccgg tcgcgataac gaaatcttct ggctgttcct
     6301 gttgcagcat catccactgc atttttacgt agtctttggc gtggccccag tcacgcaggg
     6361 aatccatatt gccgaggtac aggcacgact ccagcccctg ggcgatgttg gcgattgcgc
     6421 gggtgatttt gcgggtaacg aaggtttcgc cgcggcgcgg ggattcatgg ttgaagagaa
     6481 taccgttaca ggcgtacatg ccgtaggatt cgcggtagtt aacggtgatc cagtaggcat
     6541 acagtttggc gaccgcatac ggagatcgcg ggtagaacgg cgtggtttct ttctgcggaa
     6601 tttcctgcac cagaccgtac agttcagagg tggaagcctg atagaaacgg gttttctttt
     6661 ccagaccgag gaagcggatc gcctccagca ggcgcagcgt acccatcgcg tcgacgtcag
     6721 cggtatattc cggtgactca aaagaaaccg caacgtggct cattgcgccc aggttgtaca
     6781 cttcatccgg ctgcacttca cgcaaaatgc gcgtcaggtt ggaggtatca ctcaggtcgc
     6841 cataatgcag atggaatttc gggttgcagg tgtgcggatc ctgataaatg tgatccacgc
     6901 gctcggtgtt gaatgacgat gcacgacgct taataccatg cacctcgtaa cctttttcca
     6961 gcagaaactc tgccaggtaa gaaccgtctt gtccggttac accggtgatg agagcgactt
     7021 ttgacatgtc ttattcctct gtatttttga atttattcag tttcaacgcg ttcgcgtatc
     7081 accactgcgg gattcccccg gcaaaccaca tttgccggaa gcgatttaaa aacactgctt
     7141 cgtgcaccca cgacggtgcc gtagccaatc gtgacgcctg gggcaacaaa gacatcggtt
     7201 gccagccagc atttctcgcc aatcacaata ggcgtggcgt taatggtgaa atgttgactt
     7261 gcatggtcgt ggctaccggt gcataaataa cttttttgcg atatcaccga atgtgcgcca
     7321 atggttattt caccgagggt atataaattg acgtcatcgc cgacccacgc gtaatcacct
     7381 aaggttaatt tccacggata ggtaattttt actgacggac gaataactac gttttttcct
     7441 atttttgctc cgaataaacg taataaaaaa gcccgccagc gatacaatac ttgtggcgac
     7501 caggcaaata ttgttgcctg tactgcccac cataattgca ctttaatagc gttgccgccc
     7561 cggaaccctt tcggcaccga gaaaccgctt aaatcttgca ttggttttcc ttatattcag
     7621 actttgttat ataaggcttt cgtctttgag gtagtacgtt ggcgtaaatg ccaggataat
     7681 tcagcccaga agccaggcac atgtaatatt tgtcgttgga cttttttcgc gtcagcacac
     7741 aattccatat tattggtggt tgatacccca cccatggaaa attcagacac caggccattg
     7801 agttttttaa atgcataacc ggctttatac attttggctg ccagcgcgta gtcggaagaa
     7861 actttatatt ccaggtcata acgccatttt ttcaagccgg atacagggaa aaatatcgcc
     7921 tgatgactgg cgggcaggct gtgataaata taccagcccg gtttggcgct acgtttaatt
     7981 ttatgcccgt cgccaaaatc cagcagcgca tcgccggtga tcatcatgtt atctttttgc
     8041 atttttaact tacggacaaa atttgcggca tcctgatgaa aaatatcgcc cgaattgaga
     8101 aacaacgcga acttgccttg tgccatcgta atgcctttgt tcatggcgtc gtagataccg
     8161 ttatctggct cgctgacaaa gcgtaggtta tagataccat tgagattttc cagatactca
     8221 cgggtgccgt cattggaacc gccatcgaca acaatccatt cgaagctgat atcttccgcc
     8281 tgcgccagat gcgccagcga ggcatgtgtt ttgactatcc cttcgaggtt acgaaacgcg
     8341 acagtgatta tgctaagcaa catgttctta ttcttacctc gtaatattta acgcttttcg
     8401 caaaataaac ggacagacga ttaaaaatgc atattccggg ctaaatatcg aaccggtaaa
     8461 aaacagcgat accggagtaa aaagatataa ttgcacgcga aaattgcggt tatcaccaaa
     8521 agcgttgatc atcattttta tcactttccc catgtaccac agcgataaaa acactgcgaa
     8581 ccaggaaaaa taaataatca gcagatacaa accattgtct atggtttttc cgacatccgc
     8641 accgttaaat attccgaatg atgcgacgta ttcataaagt gagccaaatc tgactacacc
     8701 gtcaatatgg gtcaaagaat aaccgaccat caccagcgga ccgacaatac gataatacga
     8761 tgacgatcct tccgtaccta aatcgcccag acgggtggaa atataaggaa acgcgattac
     8821 cacaccaacc aggaatacag ccagagaaat caatgccaac ggtaactttt tcttaatcgc
     8881 ctctttattc agatattgaa atgcccactc cagcaaataa aacaggataa aggtcataac
     8941 ccctgaaaac gatccggata atattatccc tgcgagaatc atagcatcgg ttttaggcgt
     9001 tttgatacca aactgtttga tgctgagcca aattgagatt aatgccagag cgaaaaatgc
     9061 cggttcgaaa taaagtgctg tcgtgcgctt gccgccgaat ttaatgaaat tcagtacata
     9121 gctgttgctg taaatcagat atttcgaaat cgactccata atactgctgc caccggtgag
     9181 gataatttgc gccatctcca ccgccgccag cgccaccacc agcccgacca ccagataaaa
     9241 gaaacgtaat atcttgcgat ggttgtgtgg cgaaatcgtt ttaaagcgaa tactccacac
     9301 catgccaata atgatcacaa tatagacaaa cagcatggtt gaagtgacgt atttactggc
     9361 atccaacgac tgaccaaaca gatagttgaa cgccgtgagt cccgcaccaa tccctaaggc
     9421 aatcatcaat tttttaacgc tgatgcgttc taaaaacagc agtaacaaga caggtaaaaa
     9481 agtgacgata gtgatgggga aactttcacc aagctgggcg attttgacgt taaccagcag
     9541 gtagatcagc ggcagcagca ggtagctaca gattctgata gaagttgaca tactcctcca
     9601 gcatctgttg tccactgtag gcggcgcggc tgcgttggct gaactcagcc agcgtggtac
     9661 caaatatcgc ctgcgcgatt tccggtttgc ttaactgcac cagttgcagc acctcttctt
     9721 cactgacggt tttaccaccg gatttttgca gcacttcccg cgccgcatcg ctatgggtgg
     9781 cgatcaccgg cacgccaatc gatagcgcct cacacaaaat cagcgggtag ttatcgacgc
     9841 gagaactgaa caccagcgca tccatctgat tgagcgcgct catcaacttg cgcttgtcag
     9901 tttcaaagcc gtgattaacc acgttgccag cggtgaacgg cgagaactta ccaaaggtat
     9961 gcagttcaat tttgtcgccc agcgccatca tctgacgcac cagttgctgg ttagtcttgc
    10021 cgtcgtaacg caggtcatgc gccaccaccg cgattttcgg cttgccctgg gtttcgcgca
    10081 ccggaggcaa gtccgccaga atcgcttcgg ttgccatatc aataccatta ttgataatcc
    10141 ggcaacgccc tggcccgtac agactattga aagcgtcagc cacatgctgg ctgggggaaa
    10201 taaactgaca gcccagcgcc agcatctcgc ggaataactg gcgtttgccc gccaccagct
    10261 ggtgcgcgcg atcaatcttc accggcggat agttatttaa ggtcgggcat ttctggcagc
    10321 ccgttttcca gccttcgcaa ccgtcggtaa aggcgcagcg tccggtaacg ctccagtggt
    10381 cgtgtaacgt ccagaccaga gtgacgtccg gtttatggtt tttcaccttc tcgcaaaagc
    10441 gcaccacgct cttaagattc aaccagtagc tgtgcagcac atgcaaatgc aggaccaccg
    10501 ggcccggtgt gcgagtaatg gtgcgatata actcattgaa attgccgaac agatcgcgat
    10561 taaacagacg aaacaacgca atgttcgcca tcgcggtcat ccgcggcgta tgtttgatga
    10621 cctgcggata gttctgatgg ctgacgctct ctttgccgcc tttgccgtag ccgtagacaa
    10681 aatgtgacgc cagcccctgt tgcagcgcac gctggtgaag atctaacgcc acacctgctg
    10741 ccccgccttc cgccagtcgc acattaaatt gcaaaatatt catttaatta ccttcactcg
    10801 cgctttttct cccaccacca gcgcgttgtc cgggacagag tcgagcacca cgctgcccgc
    10861 gcccacggtg acattgttac caagcgtgat atcaccaaga ataataacgt tggcaccgag
    10921 ttcgacgccg ttgccaatgt gtggacatgc catgttatcg gcaccccgat tgccgatagt
    10981 gacgccgtgg cgaatggtga aatcatcccc cgccaccacg tttttattga tcactacggc
    11041 gtaaccgtga tggatagtaa agcggcggcc aatggtcgcg gcggcctgga tttcataacc
    11101 gaaaaagcat tcggtgataa tgcgatacag caccagcagc ggggccgccc acagattgtt
    11161 gaggacgttc tttttgcgcc acaccgaaca aaaatgagca acacgatagg caagaaccat
    11221 gcagcacggg cgtaaactcc agctgttggc acgcagatct tccagcatct ttagcgcccc
    11281 cgaataccat cagccagacg tttgccgtta cgcaccgaca gcagcgtcag caacgtgcgc
    11341 caggtcatac gtttattgcg gatctggtag agggtaaaca gctgatattt tttgcttgca
    11401 cggtcaaatt tatctttgtg cttgcggtaa aagtggaagt acccggagaa ttttttcggt
    11461 gacgaggtga tctgcatttc accgtgattg atatgcagga tctgcgtcgc ctcttccact
    11521 ttccacggtt cgccatactc caccaccatc cgcaggaaga tgtcgtaatc ctgtgctgct
    11581 ttcagttcgg tatcgaacaa gcactctttg aaacgccacg cccaggtaaa aacctgattg
    11641 ccaatgatat tgcgtttata gaacaggcgg cgtgagtacg gcgatttggg atacagtggt
    11701 aagcttgccg gttgggaata aacttctccc tggcatacgt agtcgttagc gtacaaaaag
    11761 gcgtgagtga ccagttgctg tttatgggcg aggaagacgc tcagacggtt gggtgtccat
    11821 tcgtcatcgt catcgatccc ggtaatgtat tccccttgcg ccagcataat agcctggtta
    11881 cgtaccgcgc acgccccgct gttaatatcg ttgtggatgt aagtgatacg cggatcgttg
    11941 agggcggtga cgtactgttg cagttgttcc caggaggtgg agcaatcatc cacgatgatc
    12001 atctcccagt tgctgtagtc ctggcgcagg accgatttta tcgcccgaat cgccagttgt
    12061 tggcggttcc aggttggcat atagattgag atcagcggat tatctttcat aggttgttct
    12121 cccggcttat gagctggttt gcttgccgga tgcggcgtga gcgccttatc cggcctacgg
    12181 ggcggtgcga atgcaggcgt cgttatttcg catccgactt atattcgtat tcgtaatagc
    12241 cataatcctg atacgcgctg gcgcggcgga agatggagtt cagaatcacc cctttcaccg
    12301 gaataccgtt ttgctcaaag cggctcagac tggtttccac ttctttcaat gtgttgaccg
    12361 cataacgcgc caccattaac gtggttccaa catgacgacc aacaattgcc gcatcggtca
    12421 ctgccagaat cggcggcgta tcaatcaaca ccaggtcgta gtttttactc gcccagttca
    12481 ccagttcggc aaagcgttcg ctcatcaaca gttcagaagg atttggcggt acctgaccgc
    12541 gcgggatcag gtcaaatttg gcaatagagg tcggtttagc agcggtagta atatcgccct
    12601 gaccaatcag aatttccgac aggccattga cgttattggt acccaacagc tcgtgggtgt
    12661 agcctttgcg catatcgcag tcgatcaaca acacccgttt attggtctga ctgatcaccg
    12721 ccgccaggtt ggcgcagaca aaggttttac cgattgacgg gctaacccct gtcatcatca
    12781 acacattgtt ctgcgcctgc atcatcgcga agtgcaaact ggtacgcaga ctacggatgg
    12841 cttcaatcgc cagatcggtt ggattcccca ccgccagtag ctggctctgt ttatagcgtt
    12901 taatcccttt gatggttttg acgctatcgc gcgctttttg ccattccgac agcgggatgc
    12961 tggcatagac gctgataccg tgttcttcca gtacttgcgg gctttcgatg ccgcgattaa
    13021 acaacgagcg cagcagcaca cccacgatag aaagcatcag gccgaggata atcgcgccga
    13081 ggataatcag ccctttcttc ggttttaaca cgccaggctg ggtgattgcc gggtcaacaa
    13141 tgcgcacatc gccgacggta ctggcttcgg tgattttcag ctcctgctct ttattcagca
    13201 gttgcatata gacctgctgc ccagactcga catcgcgggt cagacggaca atctcctgct
    13261 gggttttcgg catcgccgtt acgcgaccgt taagtttggc tttatcttct tccagcgcct
    13321 gacgtttctc cagcagcgtg cggtacgccg ggtgaacttt ggtgtacagc ttggagattt
    13381 ccgcctcttt aaaggtcagt tcgttcaact gcgcgtcgat gttcaccatc gaatcgagca
    13441 ccgctttcgc ttccagcggc agatcaacag aatctttatc ctgacggaag gcattcagtt
    13501 tgttttcggc aacatcaagg cggctacgta cttccggtaa ctgttgcgcg aggaaggcga
    13561 ggcttttcga cgcttccgcc gatttgcgct caatattttg ttcctgatag ttacgagcga
    13621 tgctgttaag aatgtcgcgg atctgttcgc gatcttcacc ggtataagtc aggctcagta
    13681 cgcctgcgtc tttgccgttc tccgttaccg tcaggctgtt ttgcagttga ttgatcatcc
    13741 ccagcgtgga gtatttggtg acggtaaact cactgcccgg gcgggcgtga atggcttcaa
    13801 ccatcagcgt gacgccttct tttttcagta tctggcccgc ttgcccacgg gcgctaaagc
    13861 cgccatcgct gctcagagtg tagtttttat cgtccagcac attaagcgta aacacctgat
    13921 ccgccatctc tttcgggcgg ttaaaggtag tcacttttac cgtctcgttc tgacgcccca
    13981 tcaggcgatc ccagcccgca ccgaaaatcg ggaacgtgtt tttgctcacc gcaatatcga
    14041 ggtcgagatc gtccaccgtt ttaccaagca ccaggcgcga acgaatcaac tggatctcgg
    14101 catccgatgc tggcggtttg ttggctaatg ccgatccgat atcctgcacc agcgaattgc
    14161 cgctgctttg ttcgatttgt accagtgcgt cggcgctgta tatcggcgtg gcgaagaagg
    14221 tgtaaaccac ggcacagagg gcgaatacgg cggtgatgcc aattacccac cagcgcgctt
    14281 caatgacggt gccgaccagg cgaccaatat cgatttcatc actgcccgtt accggagcgg
    14341 catgttgttt tactttttct gtcattctta tacctgctct gcgttcaatg cctgcgccca
    14401 ctggcgggca gaccgttcaa gtaatgtgta caccgctgca aacgtttccc ggcttttgcg
    14461 atacggatcg gggatttcac attcgttatc ccagtgacca aacagcatca ctttgccgcg
    14521 catctcaggt gccatctcgc ataagcgttc gatatggcgc ttttccatgg tcaaaatcag
    14581 gtcatagttg cgacacaggc ggcggctgat ttgccgtgca cagtgacctt ccagagacag
    14641 ttgatgttct gcggcgacgc tgatagcggt aggatcagcg cccttaccga ccagcgcgcc
    14701 gagtccagcg gactccactt tcagctccgg gtgataacgt tgcagtaagc gttccgccgt
    14761 cggggaacgg caaatattgc cgacacagac aactaagatg ttgttaaaca tgacgattac
    14821 cagttatgaa tgtcgctggc tgtatccgtc atgtaacgga caccgctaat agttggcagc
    14881 aactgattga tcagacggtt ccagcgggaa accggggcgg tggtgacata caccacgtca
    14941 taaggttgca ggcggaattc tgtcgccatc accagcgacg tcgcatcgga catatccagc
    15001 tggtagatat tggcaatctt gccgttacgc ccgccctcgc ctttcagcgg acgaatgaca
    15061 aagatgccgc tggcgttgga ggtggtcatg tcgatacctt cggcattgcc cagggcttca
    15121 gtcagggtca tgccgctaaa gtccattttc agggtgctct gtttcttcac ttcacccatc
    15181 acaaacactt tcagatcatc attacgcggc acgtagagaa tatcgccggg gtaaagcagt
    15241 cggttctgat tgaggtcgcc gttttgcatc agcgcctgca aagaaatgcg ttcttcacga
    15301 ccattgtgtg ttagcaccac gttgcgccag tcagcggtgt cggtcaggcc acctgcggcg
    15361 ttgatggcgt cgagaatggt cagtggtacg ttggtgatcg cctgttgacc ggatttattc
    15421 acctgacctg agatataggc cttttgcgag cggaaggcgg cgatattaac atccacctgc
    15481 gggtcagcga tgtacgtcgc taagcgcccg gtaatatcac tgcggatttc agcgagcgtt
    15541 ttcccgacta cgtggacctt gccgatatac gggtaaaaca tagtgccgtc aggctgtacc
    15601 cagttgccgg tgtcgctgga gctgcggtac tgaccggctg gcgtggtgag ttccgggtga
    15661 tcccagacgg tgacattcag aacgtcgccc ggcccgacgc gatactggta attcgcgatc
    15721 tcactttcca gcgtcatatt ggggcgcgct acattcgggc gtgggcgtaa ttggtcaatc
    15781 aggcgcgggg tcagcggata aacattcacc attttgtcga gatcgaaatc agcgtcctgc
    15841 tgtttgatga cgtctttgcc catcgtcgac atattgctgc ccggaagtac tgtgcaaccg
    15901 cttatcaagg ttactgacac caataatggc atcaatttca ttttggattt catcattgtt
    15961 tatttatcac tttggcagag taattatcct gtgcactatt aatagcaatg tcgccatgca
    16021 catttacctt gcagttaatt gaataaaaat ttaactggca tcagtcctaa aaaaattgat
    16081 ttcatccgca ggctattgac agaataattc agactggtct ttcaggcatc cagacacgct
    16141 accgcccctg gctttttagc taccaataca ctgatttagc ttaattttta aaaccctctc
    16201 aacatacagt cgttgatgag aaagggttat tgcggaaatt aacttccgaa tataaggtga
    16261 cattatggta attgaatatt ggctctccaa taatgcagga ggaagtgtta cagctaacgg
    16321 aatagcaggc aaggtaacaa ttcggtaatt ggctattttt aagaattata ttaagtgtca
    16381 ttcaatatgg tttttaggag tttctttagg ttgacaatat ttaatattgc gcctccacat
    16441 gcgatatttc ttaaataatg ttttattatt accactttta attcagggat aatgtgaggt
    16501 tattaccctc aaataatatg agataaatag tgctgcaaca ttgcattttt gccccgattt
    16561 atccatgatc gaattgtgac atttgtcata caacgaatag gttttgtact tactatggaa
    16621 tggattgccg atccgtctat ctgggccggg ttaatcacgc tgattgtgat cgaactggtc
    16681 ctcggcattg ataacctggt ctttattgcc atcctcgccg aaaaactacc gccgaagcag
    16741 cgcgaccgcg cacgggttac cgggctgctg ctggcgatgt taatgcgcct gttactgctg
    16801 gcgtcaatct cctggctggt caccctgacg caaccgctgt tcagcttccg ctcgtttacc
    16861 tttagcgccc gcgacttaat catgctgttt ggtggtttct tcctgctgtt caaagccacg
    16921 atggagctga acgaacggct ggaggggaaa gacagcaata accccacgca acgcaaaggt
    16981 gcgaagttct ggggggtggt gacacaaatt gtggtactgg acgccatctt ctcactcgac
    17041 tcggtgatta ccgccgtcgg gatggtcgac catttactgg tcatgatggc cgccgtggtt
    17101 atcgctatca gcctgatgtt gatggccagc aagccgttaa cgcaatttgt taacagtcac
    17161 ccgaccatcg ttattctctg cttaagcttc ctgttgatga ttggctttag cctggtggca
    17221 gaaggtttcg gcttcgtcat tccgaaaggc tacctgtacg ctgccattgg tttctcggtg
    17281 atgatcgagg cgctcaatca gttggctatc tttaaccgtc gacgttttct ttccgctaac
    17341 cagacactgc gccagcggac taccgaagcg gtaatgcgcc tgcttagcgg gcaaaaagaa
    17401 gatgcggaac tggacgccga aaccgcgtcc atgctggtgg atcatggtaa ccagcagatc
    17461 tttaatccgc aggaacggcg gatgattgag cgggtactta atcttaatca gcgtaccgtc
    17521 agcagcatta tgacgtcgcg ccacgatatt gagcatatcg atctcaacgc gccggaagaa
    17581 gaaatccgcc agttgctgga gcgaaatcag catacgcggg tggtggttac cgacggtgat
    17641 gacgcagaag atttgctcgg tgttgttcac gttatcgacc tgctacaaca gtctctgcgc
    17701 ggcgaaccgc tcaacctgcg ggtgttgatt cgccagccgc tggtgttccc ggaaaccttg
    17761 ccgttgttac ctgccctgga gcagttccgt aatgcccgca cgcactttgc ttttgtagtg
    17821 gatgagtttg gctcggtgga agggattgtg acattaagtg acgtcactga aaccattgcc
    17881 ggtaacttac cgaacgaagt ggaagagatc gacgcccgcc atgatattca gaaaaatgcg
    17941 gacggttcct ggacggcgaa tggtcatatg ccgctggagg atctggtgca atatgtgccg
    18001 ctgccgctgg atgaaaaacg tgaatatcac accattgccg ggctattgat ggaatatttg
    18061 cagcgtattc caaaacctgg cgaagaagtt caggtggggg attatttgct taaaacgttg
    18121 caggtggaaa gccatcgcgt gcagaaggtg cagattattc cgctgcataa ggatggcgag
    18181 ttggagtacg aggtgtgatg gtatcggtca ttttacccga tagccgaaag gaataagatg
    18241 agggaaccgc gccagcggcg atttaccgcc gggagcccgg gttgccaggg tggtggcgat
    18301 tgagccaccc tggcacgttc accggcttga gcgttccata gtagtgagga acatcaagtg
    18361 aacggaacag ccttcgatct tacatattca gaatataatg gtgccgccgc gaacgcctga
    18421 tgcgacgctt tacgcgtctt atcaggccta caggacaggt cacctggtaa cctacatctt
    18481 ctccagcaac ttcttcacat ctttgccatt gcgggaatct ttattccgct cggcccagtc
    18541 attcaggcga cgtttcgctt cgtcctgtag atgtttgcgc agtaactgat ccacttgcag
    18601 actgtaattg agttgctgcc agttgccata aacccgcagc ggtactggcg tttctttgag
    18661 gaaatcaatc agtttgcttt ccccgttcca gccacccacg acccgaatat caaactgggt
    18721 gtcgcaggtt tgatctgcca gattcaacat gccttcccct gtcagcgcca gcacaggcga
    18781 ttgaccttgc atgtcgttta acgtcacgac gccatccttc aacgtcaaat cggtggtaaa
    18841 gcggtcaaga cgcgttacgt tatcgaagtt ttcagcggcc ttcacatcac caccattacg
    18901 ttctaccgct tgctgaatca tctgctggaa gttcatccct tccatgcgcg tgtcggtcat
    18961 ttcgacatgt gcctgtcctt gccagttgtg gcggaatgcg tcggcatcta tgtcagcacc
    19021 ggagaaatca ccagccagtg acatttttcc ggtcaacgaa atcggatagt taaacgcctt
    19081 cagaatggta ccaatctcaa cgttttccag ccgtggctgg aagtttatcc gcggatttac
    19141 tgatgtcgcg tccagcgtgc ccggcagtga aacctgtcca ccgttaagtt tgccctgcaa
    19201 ctggataatc tccagcaaac ccgacttgtt ggtcatttgc gtggcaacat ctgtaaaatt
    19261 cactccgcgc cagcgcacgt tactggcctg caacaaaata tcagccgtaa agccttgcag
    19321 tccctgatat gccggttcat caatacgcga agaaatgacc gggcgcggca atgttgattg
    19381 gctctgcccc tgctgcgcgg caccgttttc accattcgct gtttcattaa gcgggatgag
    19441 gttgtcaaga ttcagttgcg ggaattgcag tctcagctgc cattcaggtt tctctgtcag
    19501 cgtgacctgt gcttgcccgc tcagcgtgct gtcattggcg gtcaagctga tttggttaaa
    19561 tgaaaggcgt ttatgtgact cctgccactg ggcctggaaa ctcccctgcc cctgaatacc
    19621 ttgttttggt aaatctgcac cctgcaactg ccagttaatt tgttcaatag ccgccgttaa
    19681 atcatgcgga taatcagaag catctaccgt accgttaagg gatatcgtga gatcgcgctg
    19741 atcgcgatta acccgcccgg agaactcaaa tgagccacga tgttggggat cttgttccat
    19801 ttgcaggcgg atattgcgga ttgtcacctg ctcgtcatct tcatgctgga acaccagcac
    19861 gctgtccgcc accttaagac tggatatatc aaacgaccat ccgcgatcgt ctgacagatc
    19921 cggcaaggta ttgtcgcgcg gtgcaaccgg agcgtcttca ctgcgcaccg cttccgtctg
    19981 cggcgtcagt tggatcactg ccccttttag catcacctgc ttaacgctca gttgatgact
    20041 cagtagtggt aaaagcgcca cgtccagacg catgttgtcg gcgcgaacca gtggctggct
    20101 tgcgccctgg gcggtgagag acattcgccc ggagaggata ctaagctgcg gccagacgtg
    20161 ccaacgcagt ggcccgtcga gctgcaattg ataaccgcta cgtgcagcaa cttgcttgac
    20221 catatagtcg cggaaatcat tcggattcac cagcaacact aacgcagata acccggccac
    20281 cagcacgacc aggagtatca tcagcgtcgt cagaaatcgt ctcatggtat cctcaatggg
    20341 cctgaattag tctttatcga ttcggctggc taccgcgccc tgctggttgc gatatttcgc
    20401 atcttcacgg cggttgtaag gtcgcgccgc cgggccggaa agcggctcaa agctcagcgc
    20461 accaattaac atgcccggac gcagcgccag cggcagctta ccggagttgt agaactccag
    20521 cacaatacag ccagaccagc ccggatcgat acggtgcgcg gtgacgtgca ccataagccc
    20581 cagacgcgcc agtgaggaac gaccgtccag ccatcccacc agatcggctg gcagcgtcac
    20641 cgactccagc gtcaccgcca gcgccagctc tcctgggtga agataaaacg cctcgctttc
    20701 atcgagtacg atctcatcgc tcatcacgcg gtcaagcgcg gcgctcactt catctttggg
    20761 accgctcaga tcgataaacg ctgccgtgtg accacggaag gtacgaaatt tattgcccag
    20821 gcgcacatcc accgtcgcgc cattaatacg ctccactggc ggacgtgggt tgatcgacaa
    20881 acggccttca tcaagccagg cttcaatatc tcggtcacac agacgcatgg cattttctcc
    20941 tttcgtgcat cactccctta acgccaatta cgtcaagggc ataccaggtt atcactgaac
    21001 ggtacacaat ttatcaagct tattcaaaga actgacttat tttcgctttc aatatatcga
    21061 tggcgatgcg gtttttcccg ccgcgcggca cgataatgtc cgcatattgt ttagaaggct
    21121 caatgaattg caggaacatc gggcgcacgg ttttttgata ttgcgccatc actgaatcca
    21181 ttgaacgccc acgctcgtta acgtcacgct tgatgcggcg catcaggcag atatccagcg
    21241 gggtatcaac gaaaattgag aagttaagtt catcacgcaa acgcgcatcc gtcagcagca
    21301 aaatgccttc gagaatgatg actttcttcg gctcaaccgt caccgtttct ttcatacgcg
    21361 tatgttcaac atagctgtaa accggcaggt caatggccga gccgcgtttc aatgcttgta
    21421 aatgctcaag cagcaggctg tgatccatcg cgctgggatg gtcgtagttg gtcttaacgc
    21481 gttcttccat cgacagatgg ctttgatctt tgtaatagca gtcttcggga attacgccga
    21541 tgtgttcatc accgacttgc tcacgtaatt cacgataaag ggtactggca ataagactct
    21601 tgccggaagc cgatgcgcca gcgataccga taatgacgca ctgatgagac tgatcagtca
    21661 tatatttagc gacctgatta acctggatgt taggaagggg gcgacgaagc gccaaacgcg
    21721 gcaattatag ggatttcatc cgcctgatac cagtcgaata gcgttgccgc gcgctcagag
    21781 taattgttga cgaataattc ccggtggcaa attacgttgt tcagttttaa tgcaaggtaa
    21841 aaattgttat acgaagttgc gcaaattatc cgcctttacg tcactttatg agcaattcgc
    21901 atataaaatg taaaactttt gtactagcat aaacacagaa acgaatactg gcgacctggt
    21961 cttgcggata aagcggtaat gagcaaacaa tcacagcatg tattaattgc cctgccccac
    22021 ccgctgcttc acctggtcag tttaggttta gtctcgttta tctttaccct tttctcgctt
    22081 gagctttcgc agtttggcac ccaactcgcc ccattgtggt tcccgacgtc catcatgatg
    22141 gtggcgtttt atcgccatgc cgggcgcatg tggccgggga ttgcgctgag ctgctcgctg
    22201 ggaaatatcg ccgcatccat cctgcttttt tccaccagct cgctgaacat gacctggacg
    22261 accatcaata ttgttgaagc cgtggtcggg gcagtgctac tgcgtaaatt gctgccgtgg
    22321 tataacccat tgcaaaatct ggctgactgg ctgcgtctgg cactcggcag cgccattgtt
    22381 ccgcctctgt tggggggtgt tctggttgtc ctgctgacgc ccggagacga tcctctcagg
    22441 gcatttttga tatgggtact ttcagaatcc atcggcgcac tggcactggt gccgctgggg
    22501 ttgttattta aaccacacta tctgctgcgc catcgcaacc cacggttgct ttttgagtcg
    22561 ctgctcacgt tagccatcac actgacgtta agctggcttt cgatgctgta tctgccgtgg
    22621 ccttttactt tcattattgt gctgttgatg tggagcgccg tgcgcctgcc acgaatggaa
    22681 gcctttttga tcttccttac cacggtgatg atggtgtcac tgatgatggc cgctgatccc
    22741 tccctgcttg ctacgccgcg tacatacctg atgagccata tgccgtggct accgtttttg
    22801 ctgatcctgc tgcccgccaa catcatgacc atggtgatgt atgcctttcg tgcggaacgc
    22861 aaacacattt ccgaaagcga aacccgtttt cgtaacgcga tggaatattc cgccatcggc
    22921 atggcattag tgggcaccga agggcaatgg ctgcaatcca acaaagcact ctgccagttt
    22981 ctcggttaca gtcaggaaga actgcgcgga ctcacctttc agcaactgac ctggccggag
    23041 gatctcaata aagatctgca acaggttgaa aagctgatca gcggtgaaat aaacacctat
    23101 tcaatggaaa aacgctacta caaccgcaat ggcgatgttg tctgggcgtt gcttgccgtc
    23161 tcactggtac gccacacgga tggcacgccg ctctatttta tcgctcagat tgaagacatt
    23221 aacgagctaa aacgcaccga acaggtgaat cagcaactga tggagcgcat cacgctggct
    23281 aacgaagcgg gcgggattgg catctgggag tgggagctaa agccgaatat ttttagctgg
    23341 gataagcgga tgttcgagct gtatgaaatt cctccgcata tcaaaccgaa ctggcaggtg
    23401 tggtacgagt gcgtgctgcc ggaagatcgc cagcacgccg aaaaagtgat tcgcgattca
    23461 ttgcaatcac gctctccctt taaactggaa tttcgcatta ccgtgaaaga cggcattcgc
    23521 catatccgcg ccctcgctaa ccgggtactg aataaagaag gcgaagtcga acgcctgctc
    23581 ggcattaata tggatatgac cgaagtgaaa cagcttaacg aggcattgtt tcaggaaaaa
    23641 gagcgcctgc acattacgct ggactccatc ggtgaagccg tggtctgtat tgatatggcg
    23701 atgaaaatta cctttatgaa tccggtggcg gagaagatga gcggctggac gcaggaagaa
    23761 gcattaggtg ttccgctcct gacggtgttg catattactt ttggcgacaa cggaccatta
    23821 atggagaaca tttacagtgc cgacacctca cgttccgcga ttgaacaaga tgtggtgttg
    23881 cactgtcgga gcggcggcag ctacgacgtg cattatagta ttacgccgtt aagtactctg
    23941 gacggcagca atataggctc ggttctggtg attcaggacg tcaccgaatc acgcaaaatg
    24001 ctgcgccagc tgagctacag cgccacccat gatgcactga cgcatctcgc caatcgcgcc
    24061 agttttgaga agcaactgcg tatcctgctg caaacggtaa acaatacaca tcagcgacat
    24121 gcactggtgt ttatcgatct tgatcgcttt aaagcggtga atgacagcgc cgggcacgcc
    24181 gctggcgacg ctttgttgcg cgaactggcg tcattgatgc tgagtatgct gcgctccagc
    24241 gacgtgctgg cgcgactcgg tggcgatgaa tttggtctgc tgctgccaga ctgtaatgtc
    24301 gaaagtgcgc gttttatcgc tacacgcatt atcagcgccg ttaatgacta tcactttatc
    24361 tgggaaggac gagtacatcg ggtaggtgcc agtgccggga ttaccttgat tgatgacaac
    24421 aatcatcagg cagctgaagt gatgtcgcag gctgatatcg cctgttatgc ctccaaaaat
    24481 ggtggacggg gccgggtgac gatttacgaa ccgcagcaag ctgccacaca tagcgaacgg
    24541 gcggcgatat cgcttgatga acagtggcgg atgattaaag aaaatcagtt gatgatgatc
    24601 gcccacggtg tcgcttcgcc gcggatcccg gaagcacgta atttgtggct gatttcactt
    24661 aagctctgga gttgcgaagg cgagattatt gatgaacaaa catttcgtcg tagcttcagc
    24721 gatccggcac ttagccatgc tcttgaccga cgggtattcc acgatttttt ccagcaggcc
    24781 gcaaaagcta ttgccagtaa aggcttaagt atcgccctcc ccctttccgt tgccggtttg
    24841 agtagcgcca cgctggtgaa tgaactaatt gagcagctgg aaaatagccc tctaccacca
    24901 cggttattac atctgattat tccggcagac gcgattttag atcacgcaga aagcgtgcaa
    24961 aaactgcggc tggcgggatg tcggatcgta ttcagtcagg tgggccgcga tctgcaaatc
    25021 ttcaactcgc tgaaagcaaa tatggcagat tacctgctac ttgatggtga gttatgcgcc
    25081 aacgtgcagg gaaatttgat ggatgagatg ctgattacga tcattcaggg gcacgctcag
    25141 cggctcggga tgaagaccat cgccgggcca gtagttttac ccctggtgat ggatacgctt
    25201 tctggcatcg gcgtcgatct gatttatggc gatgtgattg ccgatgccca accgctggat
    25261 ttgctggtga atagcagtta tttcgcgatt aactaaggcg tgcttcccca tcgcctgatg
    25321 cggcgtatca ggcctacaat tcgtccgttc cgtctggttg ccagccttcc gtataccaga
    25381 tatgcaacag cgcataagaa cgccagggct tccagcgttc ggcataacgg cggatttgtg
    25441 ccggtgtcat tcccggaaat cgctgtttaa tcagataatc atccggcagg aaaacatctt
    25501 tcgcctgcca gccacgtaag gcgaaataat tcgccgtcca gcgcccgata cccggaaaag
    25561 tttgcagcat tttcatcgcc tgctccacat cgcccggaat tgtcattgat aaggtgccct
    25621 ccagcgccgc atttgccaga tgaatcagtg cctctgcccg tttcaacggc atacctaacg
    25681 cttttaatgc ctgcgggtcg gctgctgcca gccgctgagg cgtggggaag cagacataat
    25741 ccggaaaatc atccagccgt tcgccataaa gctgtgccac tctggcggtc aattttgccg
    25801 ccatcgccac gctcaccagt tggcctaaaa tcgcccgcac gccctgctca aaagcatcaa
    25861 cacaaccagg taaacgcaat ccgggccgcg ccgcgcctaa cttgcccaac gcaccgttaa
    25921 caatctgcgg gttacattgc agatcaaaca ggcggctcat tttcgccaga cactctgcgg
    25981 caacaggttc taaacctgca cttaaattta tgtgcagagt atggcgggct atatccggaa
    26041 tagcagtcac cacgccgcga cattcgccca ccgccagact acgggcataa taatcgtcag
    26101 cgaccgtttc cacaccgttc accgcacggg cggcgagaaa tcccaacatc cacgaccagt
    26161 catacggcgg ctgccagttc aaggtataca tcgcatctcc ttgttaatcc gctttcagca
    26221 taaacgttat tcagacgcta cgctttgctt tcatattccg gttgtcgcga cggcaacatt
    26281 tcgctaaagt cacgcccctt cttcaccggc atggggatta tttcgtgttt attggttttg
    26341 attacggtac agcaaactgt tcagtggcgg tcatgcgcga cggtaagccg caattactga
    26401 aaatggaaaa cgacagcacg ctgctgcctt caatgctttg cgcgccaacg cgtgaagcgg
    26461 taagcgaatg gctgtaccgc catcatgatg ttccggcaga cgacgatgaa acgcaggcgc
    26521 tgttgcgtcg ggcgattcgt tataaccgcg aagaagatat cgatgttacg gcgaaaagcg
    26581 tgcagttcgg tctttcctca ctggcacagt acattgatga tccagaagaa gtgtggtttg
    26641 tgaaatcacc aaaatcgttc ctcggtgcca gcggcttaaa accgcagcag gtagcgctgt
    26701 ttgaggatct ggtctgcgca atgatgttgc acattcgcca gcaggcgcag gcacagctgc
    26761 cagaagcgat tactcaggcg gtgattggtc gccctatcaa cttccagggg ctgggcggcg
    26821 atgaagcaaa cacccaggcg caggggattc tggaacgcgc ggcgaagcgt gccgggttta
    26881 aggatgtggt attccagtac gagccggtcg cggctgggct ggattacgaa gccaccttgc
    26941 aggaagaaaa acgggtgctg gtggtggata tcggcggtgg tacgactgac tgttcattgc
    27001 tgctgatggg gccgcagtgg cgttcgcgtc tcgatcgtga agccagcctg ctgggtcaca
    27061 gtggttgccg tattggcggt aacgatctgg atatcgcact ggcgtttaaa aacctgatgc
    27121 cgttactggg catgggtggc gaaaccgaaa aaggcatcgc cctgccgatt ctgccgtggt
    27181 ggaatgcggt tgccatcaac gacgtacctg cgcaaagtga tttctacagt agtgccaacg
    27241 gtcgtctgct taacgatctg gtacgcgatg cccgcgaacc ggaaaaagtg gccctgttac
    27301 agaaagtctg gcgtcagcgt ttaagctatc gcctggtacg tagcgcagaa gagagcaaaa
    27361 ttgcgctttc aagcgtagcg gaaacccgcg cctcactgcc gtttatcagc gatgaactgg
    27421 ctacgctgat tagccagcaa gggctggaaa gcgccctcaa ccagccactg gcgcggattc
    27481 tggaacaggt gcaactggcg ctggataacg cccaagaaaa accggatgtt atctatctga
    27541 ccggcggtag cgcccgttca ccgctgatta aaaaagcgct gacagaacag ttgccgggca
    27601 ttccgattgc aggtggcgat gactttggct ccgtcaccgc cgggctggca cgctgggcgg
    27661 aagtggtgtt tcgttaattg aaaactggct cagcaacggc ttaaatcagc ctgcgcctga
    27721 gccagttttt ctgccgcctg acgtaacggt tccatcaacg gcatggtccg tgctggagca
    27781 tgaaggcgga atcgctgcaa ctctgtcgca ccgacagtca gcgccgattc cagccgatgt
    27841 cgtttggcgg ccatttttgc cattaccgct tgtctgtctg caggcgttac cgcttcgttc
    27901 ggcctgaaaa caaaacggcg ttcgcaactc gctttccagt cgataaccgc ctgggtcaga
    27961 tgatcaccaa aacctttcac ttgcttaacg ctccgtcggg taacgtctgc cgccgtttca
    28021 ataccaaagg accgtaacgc cgctttacgc gcagggccaa cgccgggaat agaggcaaca
    28081 tcaataaaaa atccctccag aaacttctgc ttctgccgtt cccttgcggt gtcctgaagt
    28141 gctgccagag cgcgtttctc ttcttccggt aacccgagaa tttcgtcctt cattttttcg
    28201 agcatcgccc gtttggcgat aaaaccttcc agcccgccca actgttggat ctggctgact
    28261 aaatggtcat agtccatttt cgcgcgatta aatgcctcac ggcgttgctg gatttctgct
    28321 ttataagctt tgcttgtcag actgccaata atccagatag ccgccagcac aggaaccaaa
    28381 atgatataac gcggttctgc ctgaaggccg caaagcaaca gcgataacgc tgacagtgcg
    28441 atctcaatca gaatgatgta ttcgcgccgt aacaggccta aaggaagcgg cctgccagca
    28501 gcctggaaat gatcgggtaa tggcaattgc aatgctggcg gtgctactga cgccatcacc
    28561 atcgcccaga ctttcgccag cacaaaatca ccgctggtgg taatgacctc ttcgccgaga
    28621 tcaataaaat agataacgcc ttgattatcc agcgcacacc acgggcagtc agtcaaatga
    28681 ccggggtaaa catgcattgc cgaaacggta catttcttta actgttggcg tagtaaatca
    28741 agtgccgcta cccacgcttt tgccgtcgga cgcccggttg ccacgccact ttccgtgaat
    28801 gcctgctgaa acatggcttc aacatcaccc ggtaacatcg acagcggaat agatcgtggc
    28861 ggcggtttta aaccacgtcg ctgattatct gacgcgtagg cataacggaa atgggcaata
    28921 tccgtctcca gtgcattacc cgcatcagag ataagcggta caccggaata aggatgccgc
    28981 ccaccaaaca agacgtgaaa aatcagcaac gcaaggccaa aattatcgtg attcgcggtg
    29041 cgttcaaagc cgacaaatga tggcaaggtt tgtagctctg gcggcgtaaa atgcgacacg
    29101 ccgacttcgc ataaatgcag tgtgccattg gcgttaatct gaaaggagtc gctatcaatc
    29161 aacaccactt tgctgtcacg gcctaccata aagctgttct ggtttacgtc acccacgacg
    29221 tgctcgtgaa ccgtagcaaa agatgaagca atattgcgtg caacatagag tagaaaatcc
    29281 cacgcacaat gagggtaacg ttgacgacga tgtgccgggc tatagatcat atgaatcggt
    29341 tctttaccag aaacttttgg catcataaaa ccgataacct ttccgcctcg tccaccgtga
    29401 agcgttgcct gcggccaggc gacataattc agcaactgtg cgtcagctgt cgcagccata
    29461 aaggcaagtt tgtcctgttt taaggcgggt ggcggcgtgt gataaatctt ggcgacgcta
    29521 tcgacaaact cctcgatatc ataaaccgca ccttcaccac ctttgcccag ttcacgcccg
    29581 agagtggtca attcatctgt cgatgtaaat acttttatat ttttcttcat aatgtttcaa
    29641 gggcagtcat attatatttc aatgaataat aacactgccc attataatta tttctatatg
    29701 ggaagagtcc actcaagaat aaaaatgata atgtaatttc atcaggatac aattcaattc
    29761 caacctgata atgattaact tccacaggtt ttgctaactt ttactacaca gagattctgg
    29821 agaaaaaatg aaaaagatat tacttatgtt gagtctgcta ttttttacta ccgcgggctt
    29881 tagtgaagtt tcggatacgt tagtgacggg aggttatgat aaacaagcga tgtctgacgc
    29941 cattcaacat gcgcggaaag aaactgataa attcatcgag gttatgaata aaaaagatgc
    30001 cgatactttc gcggtcaaag ccccgataac cgatcacgga agaacagagc atttctggct
    30061 tacggatgtc acttattcta acggcatgtt tattggcgta ataagcaatg accctggtat
    30121 tgttacaaac gtagaatatg gtcaggagtg gaaaatcaag aaagaagata tttcagactg
    30181 gatgtatacg cgcggggata aaatttacgg cggttatacc attgatcctt tattggtcac
    30241 ctatccaaaa gaagaagccg acgagctaag agcaaaatta gttcgctaac cagccattat
    30301 attaattgct taaactggcg gttgaactgt tgtcttcaac cgccatgcga tgttcattct
    30361 gtccacagcg ccagtgccaa cgttttatca tcatcggtac gctcgtttac cgccgggccg
    30421 gacaaaaact gctttaacaa ttcaggtaat aaatcgagtt gttcctgcgt tgctgacgcc
    30481 agcccattga aaaacggggt aaagaaaggc acatggggag aattatccag catatttaac
    30541 gccagccgct ggataccatc cgtaaatgct gcaactttgt gcgcacgctc agtgctggtg
    30601 aaagtttcca gtctggaaac cgcatcttca tcggtaatga agtgcgtcat gttggcatat
    30661 tcacccacca tcggcgtgag cggcagttgc agaccgtgcc ccaaatctac caccatgccg
    30721 ccatcgccaa tctgcataat caaggtgccg tcaggcgatg aaatcagtcc cagaaatgtg
    30781 caggcaaaat cgcgaacctc taattctttt gcctctgcct cggcaaaaag tcgctgacga
    30841 atggtcagca caatatccgt ggcgaggaca tcattaagcc ccaattcccc gccctgcact
    30901 ttttgcgaca tataagccat cgcttcattg acggcgagca tcgcgccttc gccaccctgc
    30961 gagacactgc ctgcgccatc agcgacaaat accgacaata atggctgctg gtcatttaac
    31021 caggcaacct gcatctggca ggcatcctgg caaggcaagt ccgcgctgat gtgcgacgtt
    31081 ccaacggttg atgcatagac cagacgccag ctcacactga cgtccagcct tttggcgctt
    31141 ccagcacaac ttccgttccc ggcgtggagt gggaaaccga acgcagcgaa ctggataacc
    31201 agctaaacag ttcacggaat tgtaatccct gcagtggcaa aggctgacga acgctgattt
    31261 gtgccaaagt cttcatatcc gccccctgaa caccgatgga aaagaaggca aacttcttat
    31321 cttcttcccc ctgaaacact ttgttggcgg cagcctgcca ctcatcggtt ggagcgccat
    31381 cggtgatcag gaaaatccac gggcgataat aggaaatacc attggcgcga tactcacgtt
    31441 tgcgctcctc aaccatatcc agggctttgg taatggcggc ccccattggt gtatcgccct
    31501 gggcaaacag gatgggcggg aaaaaattag cggcgctggt aaagggttgt tccacatgca
    31561 ccgggccgaa cgtcacaata ccgagttcca ctctttttaa ggccagcgaa tcggcaagca
    31621 gctcatcgcg aaaggtaacc aatccggcgt taagttcatt aatgggtcta ccgttcatag
    31681 aaccagaaac atccagcagc aaaatgcagg ggcaacgtgg ttccgggtta ctggcaaaat
    31741 cgctggtggc aaacgtgatt tgttcgctca taagaaatcc tttcttagag aattattgag
    31801 gtccggtatc ataccgactg caacaatgaa atatgagcag gcaaagcaac tttcagaatt
    31861 aggcccggcg gcgcagaaaa ttttgctatt cgcctcgcaa gaacggagag ccgtcgattg
    31921 acactaaggg cggagtgaca taatttcagg agtgagggtt agggagaggt ttccccctcc
    31981 ccctggtgtt cttagtaagc ctggaagcta atcactaaga gtatcaccag tatgatgacg
    32041 tgcttcatca taaccctttc cttattaaaa gccctcttct ccgggagagg gtttcccgtt
    32101 tcagcgtccc gctgaaatcg tcggcttacc tcctttcgcc ctgaatgcag tctatcgcta
    32161 acgcgtgaac aaaatagttt cctctgtgat tattgcgtaa cgactcgcaa agaatatttg
    32221 ctggcaggat cgcagactgc aaagcctgca gattgacaat cttatcgtga aggcataatt
    32281 tcaggagtga gggtagagcg gggtttcccc cgccctggta gtcttagtaa gcggggaagc
    32341 ttatgactaa gagcaccacg atgatgagta gcttcatcat gaccctttcc ttatttaagg
    32401 ccccttcctc gggaggggct ttcccgtttc agcgtcccgc tgaaatcgtc gacttacctc
    32461 ctttcgccct gtatgcagtc tatcgctgac ttgcggataa aatagtttcc agtgtgatta
    32521 ttgcgtaacg tctcgcaaag aatatttgct ggcaggatcg cagactgcaa agcctgcaga
    32581 ttgacaatct tatcgtgaag gcatactttc aggagtgagg gtagagcggg gtttcccccg
    32641 ccctggtagt cttagtatgc tgggtagcta atcactaaga gcaccacgat gatgatccac
    32701 ttcatcatga ccctttcctt atttaaggcc ccttcctcgg gaggggcttt cccgtttcag
    32761 cgtcccgctg aaatcatcgg cttacctcct ttcgccatgc acgtagtcta tcgctaacgc
    32821 gtagataaaa tagtttcatg tgttattact ggatgcgtgc tcgcaaatgt gcccgccatt
    32881 cagacgattc ccgatagtgt ttcataattc ctccattttt ctcccttatt ggctggctac
    32941 actagtatca ttccgcgaaa cgtttcagga agagaaactc ttaacgatga aaggtagtta
    33001 taaatcccgt tgggtaatcg taatcgtggt ggttatcgcc gccatcgctg cattctggtt
    33061 ctggcaaggc cgcaatgact cccagagtgc agccccaggg gcgacgaaac aagcgcagca
    33121 atcgccagcg ggtggtcgcc gtggtatgcg ttccggccca ttagccccgg ttcaggcggc
    33181 gaccgccgta gaacaggcag ttccgcgtta cctcaccggg cttggcacca ttaccgccgc
    33241 taatactgtt acggtgcgca gccgcgtgga cggtcaactg atggcgttgc atttccagga
    33301 aggccagcag gtcaaagcag gcgatttatt ggcagaaatt gaccccagcc agttcaaagt
    33361 tgcattagca caagcccagg gccaactggc aaaagataaa gccacgcttg ccaacgcccg
    33421 ccgtgacctg gcgcgttatc aacaactggt aaaaaccaat ctcgtatctc gtcaggaact
    33481 ggatgcccaa caagcgctgg tcagtgaaac cgaaggcacc attaaggctg atgaagcaag
    33541 cgtcgccagc gcacagctgc aactcgactg gagccgcatc accgcgccag ttgatggtcg
    33601 cgttggtctc aagcaggttg atgttggtaa ccaaatctcc agtggtgata ccaccgggat
    33661 tgtggtgatc acccagacgc atcctatcga tttgctcttt accctgccgg aaagcgatat
    33721 cgctaccgta gtacaggcgc aaaaaaccgg aaaaccgctg gtggtagaag cctgggatcg
    33781 caccaactcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc aaatcgatgc
    33841 cactaccggt acgattaaag tgaaagcacg ctttaataat caggatgatg cgctgtttcc
    33901 caatcagttt gttaacgcgc gtatgttagt cgacaccgaa caaaacgccg tggtgatccc
    33961 caccgccgct ctgcaaatgg gcaacgaagg ccattttgtc tgggtgctga atagcgaaaa
    34021 caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag tggtgatccg
    34081 cgcaggtatt tctgcgggcg accgcgtagt gacagacggc attgatcgcc tgaccgaagg
    34141 ggcgaaagtg gaagtagtgg aagcccagag cgccaccact ccggaagaga aagccaccag
    34201 ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc gagcagcaca
    34261 ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct gatggtggcg
    34321 atcttactcg ctgggattat cggttataga gccctgcccg tttcggcgct gccggaagtg
    34381 gactatccga ccattcaggt agtcacgctc tacccaggtg cgagcccgga tgtcatgacc
    34441 tctgccgtta ccgcgccgct tgaacgccag tttgggcaga tgtctggcct gaaacagatg
    34501 tcgtcgcaaa gttccggcgg tgcgtcagtt atcaccttac agttccagct aacattaccg
    34561 ctggatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa cttgttgccg
    34621 agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc accaatcatg
    34681 acgctcgccg tcacctcaac cgccatgcca atgacgcaag tggaagatat ggtggaaacc
    34741 cgcgtcgcgc agaaaatctc gcagatttcc ggtgtcggcc tggtgacgct ttccggcggc
    34801 cagcgtccgg ctgttcgtgt caaacttaac gctcaggcga ttgccgccct cggcctgacc
    34861 agcgaaaccg tgcgcaccgc tattaccggc gctaacgtta actcggcaaa aggtagcctc
    34921 gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc cgccgaagag
    34981 tatcgccagc tgatcatcgc ctaccagaac ggcgcgccga ttcgtcttgg cgatgtcgca
    35041 accgtagaac aaggcgcaga aaacagctgg ctcggcgcgt gggcgaacaa agaacaggcc
    35101 atcgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc cgacagcatc
    35161 cggcagatgc tgccacaact caccgagagt ttgccgaaat cggtaaaagt gacggtactt
    35221 tctgaccgta ccaccaatat tcgcgcatcc gtcgatgata ctcagtttga attgatgatg
    35281 gctatcgcgc tggtagtcat gattatctac ctgtttttgc gtaatattcc ggcgactatc
    35341 attcccggcg tcgcagtgcc gctgtcgtta atcggcacct tcgcggtcat ggtgtttctc
    35401 gatttttcaa tcaataacct gacgctgatg gcgttaacta tcgccaccgg atttgtggtc
    35461 gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg cgaaaaaccg
    35521 ttggcggcgg cgctcaaagg cgcgggtgaa atcggcttta ccattatctc gctgaccttc
    35581 tcactgattg cagtgttgat cccactgcta tttatgggcg atatcgtcgg gcgactgttc
    35641 cgcgaatttg ctattactct ggcagtcgcg attttgatct cagccgtggt gtcgctgact
    35701 ctgacgccga tgatgtgcgc gcggatgctc agccaggagt cgctgcgtaa acagaatcgc
    35761 ttctcccgag cctcggagaa aatgttcgac aggataatcg ccgcctatgg tcgtggactg
    35821 gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac gctgctgctt
    35881 agtgttctct tgtgggtatt cattccgaaa ggtttcttcc cggtacagga caacggcatt
    35941 attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc ccagcggcaa
    36001 cgccaggtcg cggatgtgat tttgcaggat ccggcagtgc aaagcctgac ctcatttgtt
    36061 ggcgttgatg gcactaaccc gtcgctgaac agtgcgcgtt tgcagatcaa cctcaaaccg
    36121 ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac ggcggtggat
    36181 aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg acctgaccat cgatactcag
    36241 gtcagccgca cccagtacca gtttaccttg caggccacgt cactggatgc gctcagtacc
    36301 tgggtgccac agttgatgga aaaactccag caactgccac agctttctga tgtctccagc
    36361 gactggcagg atcaagggct ggtggcgtat gtcaatgttg atcgcgacag cgccagccgt
    36421 ctggggatca gcatggcgga cgtcgataac gccctgtaca acgcgtttgg tcagcggctg
    36481 atttccacta tttatactca ggctaaccag tatcgcgtgg tgctggaaca caacactgaa
    36541 aataccccag gcctcgcggc gctggatacc attcgcctga ccagcagcga cggcggcgtg
    36601 gtgccgctaa gctcaattgc caaaattgag cagcgttttg cgccgctctc catcaaccat
    36661 ctggatcagt tcccggtaac gactatctcc tttaacgtgc cggataacta ttcgctgggc
    36721 gatgcggtgc aggcgattat ggacaccgaa aagacgctga atctgccggt ggatatcacc
    36781 acgcagttcc agggcagcac cctcgctttc cagtcggcgc tgggcagcac tgtctggctg
    36841 attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga gagctttatt
    36901 cacccgatca ccattctctc gacgctaccc acggcaggtg ttggcgcact gctggcgtta
    36961 atgattgccg gtagcgaact ggatgtgatt gcaattatcg gcattatttt gctgatcggt
    37021 atcgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga gcgcgagcaa
    37081 ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg tccgatcctg
    37141 atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttaagtac cggggtcggc
    37201 gcagaactgc gtcgtccgtt aggcatcggc atggtcggcg gcctgattgt cagccaggtg
    37261 ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc attgtggacc
    37321 aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga agttttttgc cctcttcatt
    37381 taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctatgcgg catactgggt
    37441 ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat tatggttagc
    37501 gcctcgctgc ccggtgcgtc gccggagaca atggcgtctt ccgttgccac gccgctggaa
    37561 cgctcacttg ggcgcattgc cggggtcagt gaaatgacct ccagcagttc gctcggcagc
    37621 acgcgtatta ttttgcagtt tgattttgac cgggatatca acggtgcggc gcgtgatgtg
    37681 caggcggcga tcaacgccgc acaaagtctg ctgcccagtg ggatgcccag ccgcccgact
    37741 tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tgacgctaac ctccgatact
    37801 tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tggctccgac gatttcgcaa
    37861 atcgacggcg tcggtgatgt cgatgttggc ggcagctcgc tgcccgccgt gcgcgtcggg
    37921 ctgaacccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg cactgccatc
    37981 agcaatgcca acgtgcgtaa accgcagggg gtgctggaag atggcactca ccgctggcag
    38041 atccagacca atgatgagct aaaaaccgcc gctgaatatc agccgttgat tattcattac
    38101 aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgaccgactc agtgcaggat
    38161 gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tgctgatgat ccgcaagctg
    38221 ccggaagcca atattatcca gacggttgac agcatccggg caaaattacc ggagttgcag
    38281 gagaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc caccattcgc
    38341 gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt gattctggtg
    38401 gtctttttat tcctgcgctc gggtcgcgcc accattattc ccgccgttgc ggtgccggtt
    38461 tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggtt tcagtctcaa taatctttcg
    38521 ttaatggcgc tcaccattgc tactggtttc gtggtggatg acgccatcgt ggtgctggaa
    38581 aacattgcac gtcatctgga agcgggaatg aaaccgttgc aagccgcact gcaaggtact
    38641 cgcgaagtcg gttttacggt gctgtcgatg agtctgtcac tggtggcggt gttcctgccg
    38701 ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt gacgctttct
    38761 gtcgccattg gtatttcgtt gctggtttcc ctgacgctaa cgccgatgat gtgtggctgg
    38821 atgctgaaag ccagcaagcc gcgcgagcaa aagcgactac gtggttttgg tcgcatgttg
    38881 gtagccctgc aacaaggcta cggcaagtca ctgaaatggg tgctcaatca tacccgtctg
    38941 gtgggcgtgg tgctgcttgg caccattgca ctgaatatct ggctgtatat ctcgatcccg
    39001 aaaaccttct tcccggagca ggacaccggc gtgttgatgg gcgggataca ggcggatcag
    39061 agtatttcgt ttcaggcgat gcgcggtaag ttgcaggatt tcatgaaaat tatccgtgac
    39121 gatccagcag tggataatgt caccggattt accggcggtt cgcgggtgaa cagcgggatg
    39181 atgtttatca ccctcaagcc acgcggcgaa cgcagcgaaa cggcgcagca aattatcgac
    39241 cgtctgcgcg tgaagctggc gaaagaaccg ggggcgaatc tgttcctgat ggcggtacag
    39301 gatattcgcg ttggcggacg tcagtcgaac gccagctatc agtacacgtt actgtccgac
    39361 gacctggcgg cactgcgcga atgggaaccg aaaatccgca aaaaactggc gacattgccg
    39421 gaactggcgg acgtgaactc cgaccagcag gataacggcg cggagatgaa tctggtttac
    39481 gaccgcgaca ccatggcacg gctgggaatc gacgtgcaag ccgccaacag tctgttaaat
    39541 aacgccttcg gtcagcggca aatctcgacc atttaccagc cgatgaacca gtataaagtg
    39601 gtgatggaag tggatccgcg ctatacccag gacatcagcg cgctggaaaa aatgttcgtt
    39661 atcaataacg aaggaaaagc gatcccgctg tcgtatttcg ctaaatggca accggcgaat
    39721 gctccgctgt cggtgaatca tcagggatta tcggcagcct cgaccatctc gttcaacctg
    39781 ccgaccggga aatcgctctc ggacgccagt gcagcgatcg atcgcgcaat gacccagctt
    39841 ggtgtgcctt cgacggtgcg cggtagtttt gccggcacgg cgcaggtgtt ccaggagacg
    39901 atgaactcgc aggtgatcct gattatcgcc gccatcgcca cggtgtatat cgtgctgggt
    39961 atcctttacg agagttacgt acatccgctg acgattctct ccaccctgcc ctcggcgggc
    40021 gttggagcgc tgctggcgct ggaactgttc aatgccccgt tcagcctaat cgccctgata
    40081 gggatcatgc tattaatcgg catcgtaaag aaaaacgcca tcatgatggt cgattttgcg
    40141 cttgaagccc aacggcacgg taacctgacg ccgcaggaag ctattttcca ggcctgtctg
    40201 ctgcgttttc gcccgattat gatgactacc ctggcggcgc tgtttggcgc gctgccgctg
    40261 gtgttgtcgg gcggtgacgg ctcggagctg cgccaacccc tggggatcac cattgtcggc
    40321 ggtctggtaa tgagccagct tctgacgctg tataccacgc cggtggtgta tctctttttc
    40381 gaccgtctgc ggctgcgttt ttcgcgtaaa cctaaacaaa cggtaaccga gtaaatgaca
    40441 gatcttcccg acagcacccg ctggcaattg tggattgtgg ctttcggctt ctttatgcag
    40501 tcgctggaca ccaccatcgt caacaccgcg cttccctcaa tggcacaaag cctcggggaa
    40561 agtccgttgc gtatgcacat ggtcattgtc tcttatgtac tgaccgtggc ggtgatgttg
    40621 cctgccagcg gctggctggc ggacaaagtc ggcgtgcgca atattttctt taccgccatc
    40681 gtgctgttta ccctcggttc actgttttgc gcgctttccg gcacgctgaa cgaactgttg
    40741 ctggcacgcg cgttacaggg cgttggcggt gcgatgatgg tgccggtcgg cagattgacg
    40801 gtgatgaaaa tcgtaccgcg cgagcaatat atggcggcga tgacctttgt cacagtgccc
    40861 ggtcaggtcg gtccgctgct gggtccggcg ctcggcggtc tgctggtgga gtacgcatcg
    40921 tggcactgga tctttttgat caacattccg gtggggatta tcggtgcgat cgcaacacta
    40981 atgttgatgc cgaactacac catgcagacg cggcgctttg atctttccgg ctttttattg
    41041 ctggcggttg gcatggcggt attaaccctg gcgctggatg gcagtaaagg tacaggttta
    41101 tcgccgctgg cgattactgg cctggtcgct gttggcgtgg tagcactggt gctttatctg
    41161 ctgcacgcca gaaataacaa ccgcgctctt ttcagtctga aactgttccg cactcgcacc
    41221 ttttcgctgg gcctggcggg gagctttgcc ggacgtattg gcagtggtat gttgcccttt
    41281 atgacgccgg ttttcctgca aattggcctt ggcttctcac cgtttcatgc cggactgatg
    41341 atgatcccga tggtgcttgg cagcatgggg atgaagcgga ttgttgtaca ggtggtgaat
    41401 cgctttggtt atcgtcgggt actgatagcg accacgctgg gtctgtcgct ggtcaccctg
    41461 ttgtttatga ctaccgctct gttgggctgg tactacgttt tgccgttcgt cctgttttta
    41521 caagggatgg tcaactcgac ccgtttctcc tccatgaaca ccctgacgct gaaagatctc
    41581 ccggacaatc tggcgagcag cggcaatagt ctgctgtcga tgattatgca attgtcgatg
    41641 agtatcggcg tcaccatcgc cgggctgttg ctgggacttt ttggttcgca gcatgtcagc
    41701 gtcgacagcg gcaccacaca aaccgtcttt atgtacacct ggcttagcat ggcgtttatc
    41761 atcgcccttc cggcgttcat ctttgccaga gtgccgaacg atacgcatca aaatgtagct
    41821 atttcgcggc gaaaaaggag cgcgcaatga agttctggcg acccggtatt accggcaaac
    41881 tgtttctggc gattttcgcc acctgcattg tcttgctgat cagtatgcac tgggcggtgc
    41941 gtatcagttt tgagcgtggc tttattgatt acatcaagca tggtaatgaa cagcgattac
    42001 aactgttaag tgatgcgctt ggcgagcagt atgcgcagca tggcaactgg cgcttcctgc
    42061 gcaacaatga tcgctttgtc tttcagatct tgcgttcatt tgaacacgat aattcggaag
    42121 ataaacccgg cccgggtatg ccgccgcacg gctggcgtac ccagttctgg gtggtcgatc
    42181 aaaacaacaa agtgctggtt ggtccgcgag cgccgattcc acctgacggt acacggcgac
    42241 ccattctggt caacggtgcg gaagtgggtg cggtgattgc ctcccctgtt gagcgactga
    42301 cccgcaatac tgatatcaat ttcgataaac aacagcggca aaccagctgg ctgattgttg
    42361 ctctggcaac gttactcgcg gcactcgcta cctttctgct ggcgcgtggt ttgctggcac
    42421 cggtaaaacg gctggtggac ggcacgcaca aactggcggc gggcgatttc actacccgcg
    42481 taacgcccac cagtgaagat gaactgggca aactggcgca agacttcaac cagcttgcca
    42541 gcacgctgga gaaaaaccag caaatgcggc gcgattttat ggccgatatt tctcacgaac
    42601 tgcgtacgcc attagcggtg ctgcgcggtg aactggaagc cattcaggat ggcgtgcgta
    42661 aattcacgcc ggagacggtg gcgtctttac aggcggaggt cggtacactg accaaactgg
    42721 ttgacgatct ccatcagttg tcgatgtctg atgaaggcgc tctcgcctat caaaaagcac
    42781 cggtagattt gatcccactg ctggaagtgg cgggcggcgc atttcgcgaa cgattcgcca
    42841 gccgtggcct gaaactgcaa ttttccctgc cagacagtat taccgtattt ggcgatcgcg
    42901 accgtttaat gcagttattc aataacttac tggaaaacag cctgcgctac actgacagcg
    42961 gcggtagcct gaaaatctct gccgggcagc acgacaaaac ggtgcgactg acctttgccg
    43021 acagcgcgcc gggcgtcagt gacgatcagc tacaaaaatt gtttgaacgt ttttatcgca
    43081 ccgaaggttc ccgcaaccga gccagcggcg gttccgggct ggggctggcg atttgcctga
    43141 acattgttga agcacataat ggtcgcatta ttgctgccca ttcgcctttt ggcggggtaa
    43201 gcattacagt agagttaccg ctggaacggg atttacagag agaagtatga ccgagttacc
    43261 aatcgacgaa aacacaccgc gtattttgat cgtggaagat gaaccgaagc tgggacagtt
    43321 gctcattgat tatctgcgtg ctgcgagcta tgcgccgacg cttatcagcc acggcgatca
    43381 ggtactgccg tatgtgcgcc agacaccacc ggatctgatc ctgttggatc tgatgctccc
    43441 tggcaccgat ggcttgacgc tgtgccggga aattcgtcgt ttttctgaca ttcctatcgt
    43501 gatggtgacg gcaaaaatag aagagatcga ccgcctgctc ggactggaga ttggcgcaga
    43561 tgattatatc tgtaagccgt acagcccacg ggaagtggta gcgcgcgtca aaaccatttt
    43621 gcgccgttgc aaaccgcagc gtgagttgca gcaacaggat gctgaaagcc cgttgattat
    43681 cgacgaaggt cgttttcagg cttcatggcg cggtaaaatg cttgacctga cgcctgcgga
    43741 atttcgtctg ctgaaaacgc tctctcatga accaggaaaa gtgttctccc gcgagcaatt
    43801 gctcaatcat ctttatgacg actaccgcgt agtaaccgac cgcaccatcg acagccacat
    43861 taaaaacctg cgccgcaagc tggaatctct cgacgccgaa cagtccttta tccgtgccgt
    43921 ttacggcgtc ggttaccgct gggaagccga cgcctgccgc atcgtttaac atttttacct
    43981 gccggagaag gcattcacgc cgcatccggc aacataatcc cacgcatggt cagcaaactg
    44041 acattatctc tccgccctgg caggcaccag aaaatcgctc atactttaat cggtaaacag
    44101 tccttttaga tgctgttttc gatacgcaat ttcaatcaag gagtcattat ggctggttgg
    44161 tttgaactca gtaaaagcag tgataatcaa tttcggtttg tgctaaaagc gggcaatggt
    44221 gagactatcc ttaccagcga gctgtatacc tcaaaagcct ctgcggaaaa gggcatcgcg
    44281 tcggtgcgta gcaacagccc gcaagaagaa cgctatgaga aaaaaacggc aagcaacggc
    44341 aaattctatt tcaatctgaa agccgctaat catcaaatta tcggctccag ccagatgtac
    44401 gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg cacaagccag
    44461 acggtgaaag acaatacgta attacgatgc cgggcgtggg ttgttggcgt ccggcgcttt
    44521 accagctccc ccctacagcg ctacaatgcc cgcccttaaa gtgggggcac tcccctggcc
    44581 gcttcatcag gtgaagcgga tctgacctgt catcagaacg agagaattat gtttaaaccg
    44641 gaactccttt ccccggcggg aacgctgaaa aatatgcgtt acgctttcgc ttatggcgca
    44701 gatgctgttt atgcgggcca gccgcgttac tccctgcgtg tgcgcaacaa cgaatttaac
    44761 cacgaaaatc ttcagctcgg catcaatgaa gcccacgcgc tggggaaaaa gttttatgtc
    44821 gtggtcaaca ttgcaccgca caacgccaag ctgaaaacct ttatccgtga cctgaaaccg
    44881 gtggtggaaa tggggccgga tgcgctgatt atgtctgatc cagggctgat tatgctggtg
    44941 cgcgagcact tccctgaaat gccgatccat ctctcagtac aggctaacgc cgtgaactgg
    45001 gcgacggtga aattctggca gcaaatgggt ctgacccgcg tgatcctctc tcgcgaactg
    45061 tcactggaag agattgaaga gatccgcaat caggtgccag atatggagat cgaaatcttc
    45121 gttcacggcg cgctgtgcat ggcctactcc ggtcgctgcc tgctctctgg ctatatcaac
    45181 aagcgcgacc cgaaccaggg cacctgcacc aacgcctgcc gctgggagta taacgtccag
    45241 gaagggaaag aagatgacgt cggcaacatc gtacacaagt acgaaccgat tccggtgcaa
    45301 aacgttgaac cgacgctggg gatcggcgca ccaaccgaca aagtgtttat gatcgaagaa
    45361 gcccagcgtc cgggcgagta tatgaccgcg tttgaagatg agcacggcac ttacatcatg
    45421 aactcgaaag atctgcgcgc catcgcccat gttgaacgcc tgaccaaaat gggcgtgcat
    45481 tcgctgaaaa tcgaaggccg taccaaatct ttctactatt gcgcacgcac cgcgcaggtt
    45541 taccgtaaag ctatcgatga cgcagctgcg ggaaaaccat tcgataccag cctgctggaa
    45601 accctggaag gtctggcgca tcgtggctat accgaaggtt tcctgcgtcg tcatactcac
    45661 gacgattatc agaactacga atacggttat tcagtttctg accgccagca gtttgttggt
    45721 gagtttacca gtgagcgcaa gggcgacctc gcggcggtag cggtgaaaaa taaattctcc
    45781 gttggcgaca gccttgagct gatgacgccg caagggaaca ttaactttac ccttgagcac
    45841 atggaaaacg ccaaaggcga agcaatgccg gtcgcaccgg gcgatggtta tactgtgtgg
    45901 atcccggtgc cgcaggatct tgagctaaat tacgcgctgc tgatgcgtaa tttctccggg
    45961 gaaaccacgc gtaaccccca cggtaagtga ttaatttcga ttatttttcc cggatggaaa
    46021 attcttagaa accgatcaca tacagctgca tttattaagg ttatcatctg tttcgctgaa
    46081 aaacataacc cataaaatgc tagctgtacc aggaaccacc tccttagcct gtgtaatctc
    46141 ccttacacgg gcttattttt tacgcgtaac acaatgaaat aaaaggattt atttctggtc
    46201 acgtccacac attgaccaca tcg
//
LOCUS       DAPOZO010000032        44749 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPOZO010000032 DAPOZO010000000
VERSION     DAPOZO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.032"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            179..802
                     /gene="clpP"
                     /locus_tag="ROI21_001922"
     CDS             179..802
                     /gene="clpP"
                     /locus_tag="ROI21_001922"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDX5110843.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            928..2202
                     /gene="clpX"
                     /locus_tag="ROI21_001923"
     CDS             928..2202
                     /gene="clpX"
                     /locus_tag="ROI21_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDX5110844.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            2390..4744
                     /gene="lon"
                     /locus_tag="ROI21_001924"
     CDS             2390..4744
                     /gene="lon"
                     /locus_tag="ROI21_001924"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDX5110845.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            4953..5225
                     /gene="hupB"
                     /locus_tag="ROI21_001925"
     CDS             4953..5225
                     /gene="hupB"
                     /locus_tag="ROI21_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDX5110846.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            5417..7288
                     /gene="ppiD"
                     /locus_tag="ROI21_001926"
     CDS             5417..7288
                     /gene="ppiD"
                     /locus_tag="ROI21_001926"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDX5110847.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            7439..7810
                     /locus_tag="ROI21_001927"
     CDS             7439..7810
                     /locus_tag="ROI21_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDX5110848.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            7916..8314
                     /gene="fadM"
                     /locus_tag="ROI21_001928"
     CDS             7916..8314
                     /gene="fadM"
                     /locus_tag="ROI21_001928"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDX5110849.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMIK"
     gene            complement(8366..9061)
                     /gene="queC"
                     /locus_tag="ROI21_001929"
     CDS             complement(8366..9061)
                     /gene="queC"
                     /locus_tag="ROI21_001929"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDX5110850.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(9126..10826)
                     /locus_tag="ROI21_001930"
     CDS             complement(9126..10826)
                     /locus_tag="ROI21_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDX5110851.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRAIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQIMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            10926..11744
                     /gene="cof"
                     /locus_tag="ROI21_001931"
     CDS             10926..11744
                     /gene="cof"
                     /locus_tag="ROI21_001931"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDX5110852.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLHEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            11897..12355
                     /gene="decR"
                     /locus_tag="ROI21_001932"
     CDS             11897..12355
                     /gene="decR"
                     /locus_tag="ROI21_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDX5110853.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            12385..14157
                     /locus_tag="ROI21_001933"
     CDS             12385..14157
                     /locus_tag="ROI21_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDX5110854.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRHSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            14150..15931
                     /locus_tag="ROI21_001934"
     CDS             14150..15931
                     /locus_tag="ROI21_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDX5110855.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQSRFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            16112..16450
                     /gene="glnK"
                     /locus_tag="ROI21_001935"
     CDS             16112..16450
                     /gene="glnK"
                     /locus_tag="ROI21_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDX5110856.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            16480..17766
                     /gene="amtB"
                     /locus_tag="ROI21_001936"
     CDS             16480..17766
                     /gene="amtB"
                     /locus_tag="ROI21_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDX5110857.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(17816..18676)
                     /gene="tesB"
                     /locus_tag="ROI21_001937"
     CDS             complement(17816..18676)
                     /gene="tesB"
                     /locus_tag="ROI21_001937"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDX5110858.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGNVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPLNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            18894..19466
                     /locus_tag="ROI21_001938"
     CDS             18894..19466
                     /locus_tag="ROI21_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDX5110859.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(19497..19808)
                     /locus_tag="ROI21_001939"
     CDS             complement(19497..19808)
                     /locus_tag="ROI21_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDX5110860.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            19971..20067
                     /gene="ffs"
                     /locus_tag="ROI21_001940"
     ncRNA           19971..20067
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ROI21_001940"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            20187..20540
                     /locus_tag="ROI21_001941"
     CDS             20187..20540
                     /locus_tag="ROI21_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HDX5110861.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(20582..22132)
                     /gene="pdeB"
                     /locus_tag="ROI21_001942"
     CDS             complement(20582..22132)
                     /gene="pdeB"
                     /locus_tag="ROI21_001942"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDX5110862.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVAIQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(22296..22766)
                     /locus_tag="ROI21_001943"
     CDS             complement(22296..22766)
                     /locus_tag="ROI21_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDX5110863.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVQKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(22882..23433)
                     /gene="maa"
                     /locus_tag="ROI21_001944"
     CDS             complement(22882..23433)
                     /gene="maa"
                     /locus_tag="ROI21_001944"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDX5110864.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIESTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(23605..23823)
                     /gene="hha"
                     /locus_tag="ROI21_001945"
     CDS             complement(23605..23823)
                     /gene="hha"
                     /locus_tag="ROI21_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDX5110865.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(23849..24223)
                     /gene="tomB"
                     /locus_tag="ROI21_001946"
     CDS             complement(23849..24223)
                     /gene="tomB"
                     /locus_tag="ROI21_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDX5110866.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(24769..27918)
                     /locus_tag="ROI21_001947"
     CDS             complement(24769..27918)
                     /locus_tag="ROI21_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDX5110867.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(27941..29134)
                     /gene="acrA"
                     /locus_tag="ROI21_001948"
     CDS             complement(27941..29134)
                     /gene="acrA"
                     /locus_tag="ROI21_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDX5110868.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            29276..29923
                     /gene="acrR"
                     /locus_tag="ROI21_001949"
     CDS             29276..29923
                     /gene="acrR"
                     /locus_tag="ROI21_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDX5110869.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPAITE"
     gene            30051..33413
                     /gene="mscK"
                     /locus_tag="ROI21_001950"
     CDS             30051..33413
                     /gene="mscK"
                     /locus_tag="ROI21_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDX5110870.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHANEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(33452..33616)
                     /gene="rsmS"
                     /locus_tag="ROI21_001951"
     CDS             complement(33452..33616)
                     /gene="rsmS"
                     /locus_tag="ROI21_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDX5110871.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDEASQSE"
     gene            complement(33630..34157)
                     /gene="priC"
                     /locus_tag="ROI21_001952"
     CDS             complement(33630..34157)
                     /gene="priC"
                     /locus_tag="ROI21_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDX5110872.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNMAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            34227..34604
                     /locus_tag="ROI21_001953"
     CDS             34227..34604
                     /locus_tag="ROI21_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDX5110873.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            34757..35308
                     /gene="apt"
                     /locus_tag="ROI21_001954"
     CDS             34757..35308
                     /gene="apt"
                     /locus_tag="ROI21_001954"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDX5110874.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            35437..37368
                     /gene="dnaX"
                     /locus_tag="ROI21_001955"
     CDS             35437..37368
                     /gene="dnaX"
                     /locus_tag="ROI21_001955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDX5110875.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEENGNTVCLHLRSSQRHLNNS
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    36701..36765
                     /gene="dnaX"
                     /locus_tag="ROI21_001955"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            37421..37750
                     /locus_tag="ROI21_001956"
     CDS             37421..37750
                     /locus_tag="ROI21_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDX5110876.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            37750..38355
                     /gene="recR"
                     /locus_tag="ROI21_001957"
     CDS             37750..38355
                     /gene="recR"
                     /locus_tag="ROI21_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDX5110877.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            38465..40339
                     /gene="htpG"
                     /locus_tag="ROI21_001958"
     CDS             38465..40339
                     /gene="htpG"
                     /locus_tag="ROI21_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDX5110878.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            40520..41164
                     /gene="adk"
                     /locus_tag="ROI21_001959"
     CDS             40520..41164
                     /gene="adk"
                     /locus_tag="ROI21_001959"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDX5110879.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            41296..42258
                     /gene="hemH"
                     /locus_tag="ROI21_001960"
     CDS             41296..42258
                     /gene="hemH"
                     /locus_tag="ROI21_001960"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDX5110880.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(42255..43214)
                     /gene="aes"
                     /locus_tag="ROI21_001961"
     CDS             complement(42255..43214)
                     /gene="aes"
                     /locus_tag="ROI21_001961"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDX5110881.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEACFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            43366..44670
                     /gene="gsk"
                     /locus_tag="ROI21_001962"
     CDS             43366..44670
                     /gene="gsk"
                     /locus_tag="ROI21_001962"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDX5110882.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
BASE COUNT        10967 a        11191 c        11944 g        10647 t
ORIGIN      
        1 tcatgaattt tgcatggaac cgtgcgaaaa gcctctttcg gtgttagcgt aacaacaaaa
       61 gattgttatg cttgaaatat ggtgatgccg tacccataac acagggacta gctgataatc
      121 cgtccataag gttacaatcg gtacagcagg ttttttcaat tttatccagg agacggaaat
      181 gtcatacagc ggcgaacgag ataactttgc accccatatg gcgctggtgc cgatggtcat
      241 tgaacagacc tcacgtggtg agcgctcttt tgatatctat tctcgtctac ttaaggaacg
      301 cgtcattttt ctgactggcc aggttgaaga ccacatggct aacctgattg tggcgcagat
      361 gctgttcttg gaagcagaaa acccagaaaa agatatctat ctgtacatta actccccagg
      421 cggggtgatc actgccggga tgtctatcta tgacaccatg cagtttatca agcctgatgt
      481 cagcaccatc tgtatgggcc aggcggcttc gatgggcgct ttcttgctga ctgcaggggc
      541 aaaaggtaaa cgtttctgcc tgccgaattc gcgcgtgatg attcaccagc cgttgggcgg
      601 ctaccagggc caggcgaccg atatcgaaat tcatgcccgt gaaattctga aagttaaagg
      661 gcgcatgaat gaacttatgg cgcttcatac gggtcaatca ttagaacaga ttgaacgtga
      721 taccgagcgc gatcgcttcc tttccgcccc tgaagcggtg gaatacggtc tggtcgattc
      781 gattctgacc catcgtaatt gatgccagag gcgcaactgt gccgctatac ttatccaggg
      841 cggcacaacg ctgtaagcgg cttgcgcctg agaatggcat ttgcgtcgtc gtgtgcggca
      901 caaagaacaa agaagaggtt ttgacccatg acagataaac gcaaagatgg ctcaggcaaa
      961 ttgctgtatt gctctttttg cggcaaaagc cagcatgaag tgcgcaagct gattgccggt
     1021 ccatccgtgt atatctgcga cgaatgtgtt gatttatgta acgacatcat tcgcgaagag
     1081 attaaagaag ttgcaccgca tcgtgaacgc agtgcgctac cgacgccgca tgaaattcgt
     1141 aaccacctgg acgattacgt tatcggccag gaacaggcga aaaaagtgct ggcggtcgcg
     1201 gtatacaacc attacaaacg tctgcgcaac ggcgatacca gcaatggtgt cgagttgggc
     1261 aaaagtaaca ttctgctgat cggtccgacc ggttccggta aaacgctgct ggctgaaacg
     1321 ctggcgcgcc tgctggacgt ccctttcacc atggccgacg caaccacgct gaccgaagcc
     1381 ggttatgtgg gcgaagacgt tgaaaacatc attcagaagc tgttgcaaaa gtgcgattac
     1441 gacgtacaga aagcgcagcg cgggattgtc tacatcgatg aaatcgacaa gatttctcgt
     1501 aagtcagaca acccgtctat tacccgtgac gtttccggtg aaggcgtaca gcaggcactg
     1561 ttgaaactga tcgaaggtac ggtagctgct gttccaccgc aaggtgggcg taaacatccg
     1621 cagcaggaat tcttgcaggt agatacctct aagatcctgt ttatttgtgg tggtgcgttc
     1681 gctggtctgg ataaagtcat ttcccatcgt gtagaaaccg gctccggcat tggttttggc
     1741 gcgacggtaa aagcgaagtc cgacaaagca agcgaaggcg agctgctggc gcaggttgaa
     1801 ccggaagatc tgatcaagtt tggtctgatc cctgagttca ttggtcgtct gccggttgtc
     1861 gcaacgttga atgaactgag cgaagaagct ctgattcaga tcctcaaaga gccgaaaaac
     1921 gccctgacca agcagtatca ggcgctgttt aatttggaag gcgtggatct ggaattccgt
     1981 gacgaggcgc tggatgctat cgctaagaaa gcgatggcgc gtaaaaccgg tgcccgtggc
     2041 ctgcgttcca tcgtagaagc cgcactgctc gataccatgt acgatctgcc gtccatggaa
     2101 gacgtcgaaa aagtggttat cgacgaatcg gtaattgatg gtcaaagcaa gccgttgctg
     2161 atttatggca agccggaagc gcaacaggca tctggtgaat aattaaccat tcccatacaa
     2221 ttagttaacc aaaaaggggg gattttatct cccctttaat ttttcctcta ttctcggcgt
     2281 tgaatgtggg ggaaacatcc ccatatactg acgtacatgt taatagatgg cgtgaagcac
     2341 agtcgtgtca tctgattacc tggcggaaat taaactaaga gagagctcta tgaatcctga
     2401 gcgttctgaa cgcattgaaa tccccgtatt gccgctgcgc gatgtggtgg tttatccgca
     2461 catggtcatc cccttatttg tcgggcggga aaaatctatc cgttgtctgg aagcggcgat
     2521 ggaccatgat aaaaaaatta tgctggtcgc gcagaaagaa gcttcaacgg atgagccggg
     2581 tgtaaacgat cttttcaccg tcgggaccgt ggcctctata ttgcagatgc tgaaactgcc
     2641 agacggcacc gtcaaagtgc tggtcgaggg gttacagcgc gcgcgtattt ctgcgctctc
     2701 tgacaatggc gaacactttt ctgcgaaggc ggagtatctg gagtcgccga ccattgatga
     2761 gcgggaacag gaagtgctgg tgcgtactgc aatcagccag ttcgaaggct acatcaagct
     2821 gaacaaaaaa atcccaccag aagtgctgac gtcgctgaat agcatcgacg acccggcgcg
     2881 tctggcggat accattgctg cacatatgcc gctgaaactg gctgacaaac agtccgttct
     2941 ggagatgtcc gacgttaacg aacgtctgga atatctgatg gcaatgatgg aatcggaaat
     3001 cgatctgctg caggttgaga aacgcattcg caaccgcgtt aaaaagcaga tggagaaatc
     3061 ccagcgtgag tactatctga acgagcaaat gaaagctatt cagaaagaac tcggtgaaat
     3121 ggacgacgcg ccggacgaaa acgaagccct gaagcgcaaa attgacgcgg cgaaaatgcc
     3181 gaaagaggca aaagagaaag cggaagcaga gttgcagaag ctgaaaatga tgtctccgat
     3241 gtcggcagaa gcgaccgtag tgcgtggtta tatcgactgg atggtacagg tgccgtggaa
     3301 tgcgcgcagc aaggtcaaaa aagacctgcg tcaggcgcag gaaatccttg ataccgacca
     3361 ttatggtctg gagcgcgtga aagatcgcat ccttgagtat cttgcggttc aaagccgtgt
     3421 caacaaaatc aagggaccga tcctctgcct ggtagggccg ccgggggtag gtaaaacctc
     3481 tcttggtcag tccattgcca aagccaccgg gcgtaaatat gtccgtatgg cgctgggcgg
     3541 cgtgcgtgat gaagcggaaa tccgtggtca ccgccgtact tacatcggtt ctatgccggg
     3601 taaactgatc cagaaaatgg cgaaagtggg cgtgaaaaac ccgctgttcc tgctcgatga
     3661 gatcgacaaa atgtcttctg acatgcgtgg cgatccggcc tccgcactgc ttgaagtgct
     3721 ggatccagag cagaacgtag cgttcagcga ccactacctg gaagtggatt acgatctcag
     3781 cgacgtgatg tttgtcgcga cgtcgaactc catgaacatt ccggcaccgc tgctggatcg
     3841 tatggaagtg attcgcctct ccggttatac cgaagatgaa aaactgaaca tcgccaaacg
     3901 tcacctgttg ccgaagcaga ttgaacgtaa tgcactgaaa aaaggtgagc tgaccgtcga
     3961 cgatagcgcc attatcggca ttattcgtta ttacacccgt gaggcgggcg tgcgtggtct
     4021 ggagcgtgaa atctccaaac tgtgtcgcaa agcggttaag cagttactgc tcgataagtc
     4081 attaaaacat atcgaaatta acggtgataa cctgcatgac tacctcggtg ttcagcgttt
     4141 cgactatgga cgcgctgata acgaaaaccg tgtcggtcag gtaactggtc tggcgtggac
     4201 ggaagtgggc ggtgacttgc tgaccattga aaccgcgtgc gttccgggta aaggcaaact
     4261 gacctatacc ggatcgcttg gcgaagtgat gcaggagtcc attcaggcgg cgttaacggt
     4321 ggttcgtgcg cgtgcggaaa aactggggat caaccctgat ttttacgaaa aacgcgacat
     4381 ccacgtccac gtaccggaag gtgcgacgcc gaaagatggt ccgagtgccg gtattgctat
     4441 gtgcaccgcg ctggtttctt gcctgaccgg taacccggtt cgtgccgatg tggcaatgac
     4501 cggtgagatc actctgcgtg gtcaggtact gccgatcggt ggtttgaaag aaaaactact
     4561 ggcagcgcat cgtggcggga ttaaaacagt gctaattccg ttcgaaaata aacgcgatct
     4621 ggaagagatt cctgacaacg taattgccga tctggacatt catcctgtga agcgcattga
     4681 ggaagttctg actctggcgc tgcaaaatga accgtctggc atgcaggttg tgactgcaaa
     4741 atagtgacct cgcgcaaaat gcactaataa aaacagggct ggcaggctaa ttcgggcttg
     4801 ccagcctttt tttgtctcgc taagttagat ggcggatcgg gcttgccctt attaaggggt
     4861 gttgtaaggg gatggctggc ctgatataac tgctgcgcgt tcgtaccttg aaggattcaa
     4921 gtgcgatata aattataaag aggaagagaa gagtgaataa atctcaattg atcgacaaga
     4981 ttgctgcagg ggctgatatc tctaaagctg cggctggccg tgcgttagat gctattattg
     5041 cttccgtaac tgaatctctg aaagaagggg atgatgtagc actggtaggt tttggtactt
     5101 ttgccgttaa agagcgtgct gcccgtactg gccgcaaccc gcagaccggt aaagagatca
     5161 ccatcgctgc tgctaaagta ccgagcttcc gtgcaggtaa agcactgaaa gacgcggtaa
     5221 actaagcgtt gtccccagtg gggatgtgac gaagttcaag ggcgcatcta ctgatgtgcc
     5281 ttttttattt gtattcggtg actttctgcg tcttgtgggc tgacaattgc ccccgtttct
     5341 tgtcacaata ggcctttgcg cgcatcgata cgttgcgtga ggtacacagt catctacagc
     5401 ggagtgttgt tacaccatga tggacagctt acgcacggct gcaaacagtc tcgtgctcaa
     5461 gattattttc ggtatcatta tcgtgtcgtt catattgacc ggcgtgagtg gttacctgat
     5521 tggcggaggc aataactacg ccgcaaaagt gaatgaccag gaaatcagcc gtgggcaatt
     5581 cgagaatgcc ttcaacagcg agcgtaatcg catgcagcaa cagctgggcg atcaatactc
     5641 tgagcttgca gcgaacgaag gctatatgaa aaccctgcgt caacaggtgc tgaatcgtct
     5701 gatcgacgag gcgctgctgg atcagtacgc tcgtgaactg aaactgggta tcagcgatga
     5761 gcaggttaaa caggcgattt tcgcgacccc agccttccag gttgacggca aatttgataa
     5821 cagccgctat aacggtatcc tcaaccagat ggggatgacc gccgatcagt acgcccaggc
     5881 gctgcgtaac cagctcacta cccaacagct gattaacggc gttgccggta ccgattttat
     5941 gctgaaaggt gaaaccgacg agctggcggc actggtagct caacaacgcg tggtgcgtga
     6001 agcgactatc gatgttaacg cgctggcggc gaagcagcct gtgaccgaac aggaaattgc
     6061 cagctactac gaacaaaaca aaaacaattt catgacgccg gaacaattcc gcgtgagtta
     6121 catcaagctg gatgccgcaa cgatgcagca accggttagc gatgcggata tccagagcta
     6181 ctacgatcag catcaggatc aattcaccca gccgcagcgt acccgttaca gcatcatcca
     6241 gaccaaaact gaagatgaag cgaaagcggt acttgatgag ctgaataagg gcggtgattt
     6301 tgctgcatta gccaaagaaa aatctgccga tattatctct gcccgtaacg gcggtgatat
     6361 gggttggtta gaagatgcca ctatcccgga cgaactgaaa aatgcgggtc tgaaagaaaa
     6421 aggccaactg tctggtgtta tcaaatcttc ggtcggtttc ctgattgtac gtctggacga
     6481 cattcagcca gcgaaagtga aatcgttaga cgaagtacgt gacgacattg cggcgaaagt
     6541 gaaacacgaa aaagccctcg atgcgtacta cgcgctgcag cagaaagtga gcgatgcggc
     6601 aagcaacgac accgagtctc tggccggtgc agagcaagct gccggcgtta aagccactca
     6661 gacgggttgg ttcagcaaag ataacctgcc ggaagagttg aacttcaagc cagttgccga
     6721 cgctatcttc aacggcggtc tggtaggtga aaacggcgcg ccgggcatca actctgacat
     6781 catcaccgtc gatggcgacc gcgcattcgt gctgcgcatc agcgagcaca aaccggaagc
     6841 ggtgaaaccg ttggcagatg ttcaggaaca agttaaggca ctggttcagc acaacaaagc
     6901 tgaacaacag gcgaaagtgg atgctgagaa actgctggtt gatttgaaag ccggcaaagg
     6961 tgcggaagct atgcaggctg ccggtctgaa atttggcgag ccgaaaacct taagccgttc
     7021 cggtcgtgac ccgattagcc aggcggcgtt tgcactacca ctgccagcga aagacaaacc
     7081 gagctacggt atggcgaccg atatgcaagg taatgtggtt ctgctggcgc tggatgaagt
     7141 gaaacaaggt tcaatgccgg aagatcagaa aaaagcgatg gtgcagggta tcacccagaa
     7201 caacgcacaa atcgtctttg aagctctgat gagtaacctg cgtaaagagg cgaaaatcaa
     7261 aattggcgat gcgctggaac agcaataatc ctgaagccgc ctcgcaaaaa aatgctttgc
     7321 agctgtaaca agaaaaggtc gctttcgcgg ccttttccat ttctgaacat tgccatttgt
     7381 ttactgtttt cactgccgtt aaggtgattc cactgttaac aaacaaggag aaaacagtat
     7441 gaaacacgga attaaagcac tgctcattac cctgtctctg gcttgtgccg gaatgtctca
     7501 tagcgcgctg gcggcagctc ctgcggcgaa accgacaacg gtagaaacca aagcggaagc
     7561 tcctgcagca caaagtaaag cagcagtacc ggcgaaagcc agtgacgaag aaggcacccg
     7621 ggtcagcatt aataatgcca gcgcggaaga gttagcccgc gcgatgaatg gcgttggcct
     7681 gaagaaagcg caggcgattg tcagttatcg cgaagagtac ggtccgttta aaacggtgga
     7741 ggatctcaag caggtgccgg ggatgggcaa ttcgctggtg gaacgtaatc tggcggtatt
     7801 aaccctgtaa ttaatttgca tagtggcaat tttttgccag actgaagagg tcataccagt
     7861 tatgacctct gtacttataa cagtttttat aaaaacaacg taaggttatt gcgctatgca
     7921 aacacaaatc aaagttcgtg gatatcatct cgatgtttac cagcacgtta ataacgcccg
     7981 ctatctggag tttctcgaag aagcccgctg ggatgggttg gaaaatagcg acagttttca
     8041 gtggatgacg gcccataaca tcgccttcgt cgtggtcaat atcaatatta actatcgtcg
     8101 cccggcggta ttaagcgacc tgttaaccat taccagtcag ttgcagcaat taaacggtaa
     8161 aagcggcatc ttaagccagg tcattacact ggagccggaa gggcaagtgg tagcggatgc
     8221 gcttattacg tttgtttgta ttgatcttaa aacgcagaaa gcattagctc tggaagggga
     8281 attgcgcgaa aagctggagc agatgattaa gtaaacgttt tgtggtgccg gatgctcaag
     8341 ccgcatccgg cgacacccgg aataattact tcaacccggt tttctgcttc atcgctgcca
     8401 tcaccgtcgg tttatcggcc agataatgat tcaaaccgtt ggcgcgtaaa ttacatgccg
     8461 cacaatgacc gcaaccgtcg cctttaatgc cgttatagca ggtcaacgtt tcgttacgga
     8521 ctaaatccag tttgccgtaa taatctgcca gcgcccaggt ttccgcttta tcaatccaca
     8581 tcagcggcgt ttcaaaacga atatctttcg ccatgcccaa actgacggca tggtttagtg
     8641 ctttcacaaa ctcatcgcgg caatccgggt agccggagaa atccgtttca cagacgccgg
     8701 taatcaccgc ttctgctttt acctgatacg catatattgc cgccagcgtc aggaacaaaa
     8761 tattacgccc agggacaaac gtattcggga tgccatcagc ttcaggttca taatcaggca
     8821 ccggaatgct gtcacgcgtc aggctactga ccgccagctc gttgagcagc gttacatcaa
     8881 gaaccttatg cgcgcgtgcc cccagtttca gcgccagttc gcgtgccacg tcgatttctg
     8941 cgcgatgtcg ctgaccgtaa tcgaacgtca cgcaatggac ttcatcatat tgttgtaatg
     9001 cctgcaccag acaggtggtg gaatcctggc ctccactgaa cacaacgaca gcacgtttca
     9061 tagataatcc tgcttgaaca ataaaagcgt tatggtaacg cctgcgatta acccggacca
     9121 gctattcatt gcgacggcgc gggaagccag gcttcggtaa attcaaacca gccgcgcggt
     9181 gtcagtcgca caccgttcac gccgggaggg gcactaatgc gatagtgata gttgaacagc
     9241 ggcgtcagcg tcgcatctgt cattaactgg ctaaaaaccg ctttcagggc attaaatcgg
     9301 ttttcttcat caggcattat ttgcacggca tccagtgtcg attgtagatg tgcgtatgct
     9361 ggagcgtcga aaacatgtgg ccacagcgga tcgcagcgca gccattgctc cagagtatat
     9421 tccggtgctt cgccaattaa tctgtcgccc atcatgaggt ctgcgtgtgc cagtagggtc
     9481 gtgtcgtccc agttttttgc gttatgaaaa ataattgtga gttcacagcc ttccgctgcc
     9541 agtgtcgcct gtaggcgttc tgccatggta tgaagttcta tcggtaggtg ataaaccagc
     9601 gtcaaggttt tcggtagttt gacttcatcc ggtacttgcc aatgcggaat agtccagcct
     9661 ggcagtaatg catgactggc ggtgatcagg ttttctccga cttctaacgt ttgtaataaa
     9721 ccggattgat gaataatgga gatcactttt cgcgcctgcc agagggagag tcggggactt
     9781 ttgcgcaacg ttaaatagca aaaacctaaa ctgatgccac tactgacctg gctgacccgt
     9841 tgcagctcct ccggtttgcc gatggtgatt tgcacgggat gccgacaact ggttcccaaa
     9901 tctttttcga aaagcggcgg agttatccag tactcaaccg ctttaagcag cggatgacgt
     9961 aaatggtaat aatcatgact ctccaggcgc accagctccg ctgtgaattg tgttaagcga
    10021 aaaggacccg tgccgatcag tgggaattgc ggatgcgcca gatggctgca atagctcgcc
    10081 agccggtgcg caagccagta atcagggcga tgtaaaaaga aggtcagaca ctgcggatgg
    10141 gtgacttcaa tacgcttcac gctaataaat aattgatcca gtgctggcag ttgtaacagc
    10201 atcaataatc gctggtgtaa gtgtgaggct tttactgcat cgccgttatg ccagtgtagg
    10261 gttgaacgaa gataaaagtc ccagcgtaac ccgtcagcag aggtttccca gtgatgcgct
    10321 aaatcgccaa tcgcgcgctg ggtattatta tcgaagcggg tcaggccgga aaatatctgt
    10381 ccggcgagat gctgctcggc acggccgggc aaaaagcccg gttgtagcgg ttcgagcggg
    10441 cgatagtagg gaatacgcaa cgtgggcgta tcgttttgcc attgtccgcc cataaacggc
    10501 tgtaacagag tgcgcagctc acctggggcc agttgcgcca gctccagcac atcttgctgc
    10561 tttccggttt ccagtgcctt ttccatcatc gcattgcgta gcgattctgg cgtgaccaga
    10621 aagcgtaatt gtccgcgctt tccgcgtcct gactgcgcct gccactccag ccatcccgcc
    10681 tcctgtgcct ggcgtaacag cgtacgaaca tggcgttcgc tgcaaaaaca gcgttcggcc
    10741 agttcgctga cggtgacggt ttgcggcttt ccggcggaag gttgccacag acgttgatac
    10801 tggttaagac ggttgagcag tcgcatataa acccggaaca atattattta actattcact
    10861 attacttccg tatatatcag gtgatactca atcaccatta accgtgtcac agagtggaga
    10921 aagaaatggc tcgtctggca gcatttgata tggatggcac tttattgatg cctgaccatc
    10981 atttaggtga gaaaaccctc tctactttgg cgcgactgcg tgaacgcgac attaccctca
    11041 cttttgccac ggggcgtcat gcgctggaga tgcagcatat tctcggggcg ctatcgctgg
    11101 atgcatattt gattaccggc aacggaacgc gcgtgcattc tctggaaggt gaacttttac
    11161 atcgtgatga tttacctgcg gatgtcgcgg agctggtgct gtatcagcaa tgggataccc
    11221 gagccagcat gcatatcttc aatgacgatg gttggtttac cgggaaagag atccctgcgt
    11281 tgttgcaggc atttgtctat agcggttttc gttatcagat aatcgatgtc aaaaaaatgc
    11341 cactcggcag cgtcaccaag atctgcttct gtggcgatca cgacgatctt acacgcttgc
    11401 agatccagct acacgaagca ttaggcgagc gtgcacattt atgtttttcc gccacggatt
    11461 gcctcgaagt gctgccggtg ggctgcaata aaggcgcagc attgacggtg ctgacccaac
    11521 atttaggttt atcgttgcgc gattgcatgg cctttggtga tgcgatgaac gatcgcgaaa
    11581 tgttaggcag cgtcggtagc ggctttatta tgggcaatgc gatgccgcaa ctgcgcgcgg
    11641 aactcccgca tttaccggtg attggacatt gccgaaatca ggctgtctct cactatttga
    11701 cgcactggct ggactatcca catctacctt attcccccga ataacgagat cccttccagc
    11761 accgggcaat tgcccggttt tttttgcgtt gaatttgtca ttttgtgccg tggtgtttaa
    11821 accgcacaga ataaattgtc gcgatttcac ctttaaaata gaattaaaag agaaaaaaat
    11881 tctccgtgga agggctatgt tagataaaat tgaccgtaag ctgctggcct tactacagca
    11941 ggattgcacc ctctctttgc aggcactggc tgaagccgtt aatctgacaa ccaccccgtg
    12001 ctggaagcgt ctgaaacggc tggaggacga cggtatcctt atcggcaaag tcgccctgct
    12061 ggatccggaa aaaataggcc tcggcctgac cgcctttgtg ctgataaaaa cgcaacatca
    12121 cagcagcgaa tggtattgcc gctttgtcac ggtggttacc gaaatgccag aagtgctggg
    12181 gttctggcgc atggccggtg aatacgatta tctgatgcgc gtccaggttg ccgacatgaa
    12241 acgctacgac gagttttata agcgtctggt aaacagcgtg ccggggctgt cggacgtcac
    12301 ttccagcttc gcgatggaac agattaaata caccacttct ttacccatcg aataaatatc
    12361 cagaatcagg tcaggacaca acgcgtgcga ttatttgctc aattaagctg gtatttccgt
    12421 cgggaatggc gtcgctatct cggggctgtc gccttgcttg tcattatcgc gatgctgcaa
    12481 ctggttccgc caaaagtggt tggtattgtt gtcgatggcg tgacagaaca acactttact
    12541 accgggcaga tcctgatgtg gatcgccacc atggtgctga ttgccgttgt ggtttatctc
    12601 ctgcgttacg tctggcgcgt attgctgttt ggtgcgtctt atcaactggc tgttgaactg
    12661 cgtgaagatt attaccgtca gctaagccgg cagcatcctg agttttacct gcgtcatcgc
    12721 acgggtgacc tcatggctcg tgcgaccaat gacgtcgatc gcgtcgtgtt tgccgccgga
    12781 gaaggggtgc tgacgctggt ggattcactg gtgatgggct gcgctgtgtt gattatgatg
    12841 tctacgcaaa ttagctggca gttgacctta tttgccctgt tgccgatgcc agtgatggcg
    12901 atcatgatta agcgcaacgg cgatgccttg catgaacgct ttaagctggc acaggcggcg
    12961 ttttccagtc ttaatgaccg cacccaggaa agcctcacca gtatccgtat gatcaaagcc
    13021 tttggtctgg aagatcgcca gtcggcgtta tttgccgcgg atgccgaaga taccggcaaa
    13081 aaaaacatgc gggtggcgcg tattgatgct cgtttcgacc cgaccatcta tatcgcgatt
    13141 ggtatggcga acttgctggc gattggcggc ggtagttgga tggtggtgca gggtagttta
    13201 acgctgggcc agctcaccag ttttatgatg tatttaggtc tgatgatttg gccaatgctg
    13261 gcgctggcat ggatgtttaa catagtggaa cgtggtagtg ctgcgtacag ccgcattcgc
    13321 gcgatgctgg cggaagcgcc ggtggtgaat gatggtagcg aacccgtgcc ggaaggtcgt
    13381 ggcgaactgg atgtaaatat tcgccagttc acgtatccgc agactgacca tcctgcgctg
    13441 gaaaacgtca atttcgccct gaaacccggt cagatgctgg gtatttgcgg gccgactggt
    13501 tccggcaaaa gtaccctgtt gtcgctcatt cagcgtcatt tcgacgtcag cgagggggat
    13561 atccgctttc atgatattcc tctgacgaag ttacaactcg atagctggcg tagccgcctg
    13621 gcggtagtta gccagacacc gttccttttt tccgacaccg tagcgaacaa catcgcgttg
    13681 ggttgcccga atgccaccca gcaagagatt gagcatgtcg cgcggttagc cagcgtgcat
    13741 gacgatattt tgcgtttacc gcaaggttac gatacagaag tgggcgagcg cggcgtgatg
    13801 ctttccggtg ggcaaaaaca gcgtatctcc attgctcgtg cgttattagt caacgcggaa
    13861 atcctcatcc ttgatgatgc gctttcggcg gtggacggac gcacagagca ccagatcctg
    13921 cataacctgc gtcagtgggg gcagggaaga acggtaatca tcagtgccca tcgccattcg
    13981 gcactgacgg aagccagtga aattattgtg atgcagcacg gacatatcgc tcagcgtggc
    14041 aatcatgatg agctggcgca acaaagcggc tggtatcgcg atatgtatcg ctatcaacaa
    14101 ctggaggcgg cgctcgacga cgctccggaa attcgcgagg aggccgttga tgcgtagttt
    14161 tagccaactg tggccgactc tcaagcgtct gttagcgtac ggttcgccgt ggcgtaaacc
    14221 gctggggatt gcggtcctga tgatgtgggt tgcggcagcg gcagaagtca gtgggccgct
    14281 gcttatcagc tattttatcg acaatatggt agcgaaaaat aacctgccgt tgaaagtggt
    14341 tgcagggctg gctgcggcgt atgttgggct gcaactgttt gccgccgggc tacattacgc
    14401 gcagtcgctg ctgtttaatc gggcggcagt aggcgtagtg caacagttgc gtaccgacgt
    14461 gatggatgct gcgttacgcc agccattaag cgagtttgat acccaacccg tcgggcaggt
    14521 gatttcccgc gtcactaatg acactgaagt gatccgcgac ctctacgtta ccgtagtggc
    14581 aactgtcctg cgtagcgccg cactggtggg cgcgatgctg gtggcgatgt tcagcctcga
    14641 ctggcgaatg gcgctggtgg cgataatgat tttcccggtg gtgctggtgg taatggtgat
    14701 atatcagcgt tacagcacgc cgatcgtccg ccgtgtacgc gcttatctgg cagatatcaa
    14761 cgacggcttt aacgaaatca tcaatggcat gagcgttatc cagcagttcc gtcagcagtc
    14821 gcgatttggc gaacgtatgg gtgaggccag tcgttcacac tatatggcga ggatgcaaac
    14881 cctgcgcctc gacggttttc tgctgcgtcc gctgctgagt ctgttttcat cgctcattct
    14941 ttgtgggttg ttgatgctgt ttggcttctc cgccagcggc accattgaag tgggcgtgtt
    15001 gtatgcattt atcagctatc ttgggcgact taacgaacca ttaatcgaac tgaccacgca
    15061 gcaggcgatg ctgcaacagg ctgtagttgc tggtgagcgt gtgtttgaac tgatggacgg
    15121 accgcgccag caatatggca atgatgatcg cccgttacag agcggcacca tcgaagtcga
    15181 taacgtgtca tttgcttatc gcgatgacaa tctggtgcta aagaacatta atctctctgt
    15241 gccttcgcgt aactttgtgg cactggtcgg gcataccggc agtggcaaaa gcaccctcgc
    15301 cagtttattg atgggctatt acccgctaac ggaaggtgag attcgccttg atggtcgtcc
    15361 attaagttcg ttaagtcaca gcgcgctgcg tcagggcgtg gcgatggtgc agcaagatcc
    15421 ggtagtgctg gcggatacct ttctcgccaa cgtgacgctg ggacgtgata tctccgaaga
    15481 acgcgtctgg caggcgctgg aaaccgtgca actggcggag ctggcgcgca gcatgagtga
    15541 tggtatttat acgccgctgg gagagcaggg gaataatctc tccgtcgggc aaaagcaact
    15601 actggcactg gcacgcgtgc tggtggagac gccgcaaatc ctgatccttg atgaggcaac
    15661 cgccagcatt gactccggta ctgaacaggc gattcaacat gctctggcgg cggtgcgtga
    15721 gcataccacg ctggtggtga ttgctcaccg cttatcaact attgttgatg ccgacactat
    15781 tctggtgctt catcgtgggc aagccgtgga gcagggcact caccagcaac tgctagcggc
    15841 ccagggacgc tactggcaga tgtatcaact gcaacttgcg ggcgaagagc tggcagccag
    15901 cgtgcgtgaa gaggaatcat tgagcgcctg aatagcgcaa tatttcatcg ttggtgcaaa
    15961 aatgtaacgc actgtgcact gtcatagtgc gttttcattt tcaatcttct taacttcctg
    16021 ctctctttct cgtttttcat ttctggcaca ccgcttgcaa taccttcttc gtgtagcaga
    16081 accattaccg aattctgacc ggaggggatc tatgaagctg gtgaccgtga taatcaaacc
    16141 attcaagctg gaagacgttc gtgaagcgtt atcttccatt ggtattcagg gcctgaccgt
    16201 caccgaagtg aaaggtttcg ggcgtcagaa agggcatgcc gagctgtacc ggggagcgga
    16261 atacagcgtc aacttcctgc caaaagtaaa aattgatgtg gcgattgctg atgaccaact
    16321 cgatgaagtg atcgatatcg tcagtaaggc ggcttacacc ggaaaaattg gcgacggcaa
    16381 aatcttcgtc gctgaattgc aacgcgtcat tcgtattcgt accggcgaag ccgacgaagc
    16441 ggcgctgtaa tctctggcac acagcaacag gaacgaaaaa tgaagatagc gacgataaaa
    16501 actgggcttg cttcactggc gatgctgccg ggactggtaa tggctgcacc tgcggtggcc
    16561 gataaagccg acaatgcgtt tatgatgatc tgtactgcgc tggtgctgtt tatgactatt
    16621 ccggggattg ccctgtttta cggtgggttg attcgcggca aaaacgtgct gtcgatgctg
    16681 acgcaggtga cggtgacgtt tgcactggtc tgtattctct gggtggttta cggttactcg
    16741 ctggcgtttg gtgagggcaa caacttcttc ggcaacatta actggctgat gctgaaaaac
    16801 atcgaactga cggcggtgat gggcagcatt tatcagtata tccacgtagc gtttcaggga
    16861 tcgtttgcct gcattaccgt aggcttgata gttggagcac tggcggaacg aatccgcttc
    16921 tcagctgtgt tgattttcgt ggtggtatgg ctgacgctct cctacattcc gattgcgcat
    16981 atggtctggg gcggcggttt gctggcttct cacggtgcac tggatttcgc gggtggcacc
    17041 gtggtgcaca ttaacgccgc aatcgccggt ctggtgggcg catatctgat aggcaaacgc
    17101 gtgggcttcg gcaaagaggc gtttaaaccg cacaacctgc cgatggtctt caccgggact
    17161 gccattctct atatcggttg gtttggcttt aacgccgggt cagcgggcac ggcgaatgaa
    17221 atcgcagcac tggcatttgt gaatactgtg gtcgcaacgg cggcggcaat tcttggctgg
    17281 atcttcggtg aatgggcgct gcgtggtaag ccttcactgc tgggagcgtg ttcaggcgcg
    17341 attgccggtc tggtcggcgt gacgccagcc tgtggctaca ttggggttgg cggcgcgttg
    17401 attatcggcg tggtagctgg tctggcgggc ttgtggggcg ttaccatgct caaacgcttg
    17461 ctgcgggtgg atgatccctg cgatgtcttc ggtgtgcacg gcgtttgtgg cattgtcggc
    17521 tgtatcatga ccgggatttt tgccgccagt tcgctgggtg gcgtgggctt cgctgaaggt
    17581 gtgacgatgg gccatcagtt gctggtacag ctggaaagca tcgccattac gatcgtctgg
    17641 tccggtgtgg tggcatttat tggctacaaa ctggcggatc tgacggttgg cctgcgtgtg
    17701 ccggaagaac aagagcgaga agggctggac gtcaacagcc acggcgagaa tgcctataac
    17761 gcgtaacaag cactgcaaaa aaacagccgg acggttttca cctccggcta tttttttaat
    17821 tgtgattacg catcacccct tcctgaacgg tcgaggcaac cagcacgcca tcctgggtat
    17881 aaaactcacc gcgcacaaag ccgcgagcgc tggacgccga ggtgctctcc acgctataca
    17941 gcagccattc attcaaattc aacgggcgat ggaaccacat ggaatggtca atggtggcaa
    18001 tctgaatccc cggttcgaga aaaccgatgc cgtgcggctg taaagctacc ggcaggaaat
    18061 taagatcaga agcgtaaccg agcagatact gatgaacgcg cagatcatcc ggcacgttac
    18121 catttgcgcg gatccacacc tgacgatgcg gttctgcgac gtgccctttc agtgggttat
    18181 gaaactccac cggacggact tcaagcggac gatcgcagat gaatttatct ttcagcactg
    18241 gcggcagcag gtgcgccagc gattgggcga tttgcgtttc cgaaggcagg ccatcaggcg
    18301 ctggcgcgga cggcattgtt ttttgatgtt cgaaacccgc ttctggtgcc tggaaagagg
    18361 cagtcatata aaaaatcggt ttgccgtttt gaatagcagc aacccggcgg gcgctgaagc
    18421 tgttaccgtc acgcagcgtt tcgacatcat aaataatcgg cttcttacta tcgcctgggc
    18481 gaagaaagta gctgtgaaac gaatgtacca gccgctcttc agggacggtc tcttttgcag
    18541 catacaaggc ctgacccacg acctggccgc caaacacctg gcgtaaacct aaatcttcac
    18601 tctggccgcg aaagagtcct tcctcaattt tttccagatt taacaatgtc agtaaatttt
    18661 ttagcgcctg actcatataa ctctccagta acaaagctgc cgcagcaagc caaagtgagt
    18721 tgagtataac gcaaatttgc cactggtccg atgggggcaa tggtctgaat tacgggctaa
    18781 ttacaggcag aaatgcgtga tgtgtgccac acttgttgac gttactattt tgttaaccac
    18841 tcttccggcg aggaaagtta gcccgctggt gcattgataa taaggagaag tgaatgaaac
    18901 tcgtgcacat ggccagtggt ttagcggttg cgattgcgtt ggcggcttgc gcagataaaa
    18961 gcgcggatat tcagacgcca gcaccggcgg caaatacgtc tatttcagca acacaacaac
    19021 ctgctatcca gcaaccgaat gtctccggta ccgtctggat ccgtcagaaa gtcgcactgc
    19081 cgcctgatgc tgtgctgacc gtgacacttt ctgacgcgtc gttagccgat gcaccgtcaa
    19141 aagtactggc gcagaaagcg gtgcgtaccg aaggcaaaca gtcaccattc agctttgttc
    19201 tgccatttaa tccggcagat gttcagccga atgcgcgtat tctgttgagt gcggcgatta
    19261 ccgtgaatga caaactggta tttattaccg ataccgttca gccggtgatc aaccagggcg
    19321 gaactaaagc cgacctgaca ttggtgccgg tgcagcaaac cgccgtgccg gttcaggcca
    19381 gcggtggcgc aacgactact gtaccttcga cttcaccaac tcaggtgaat ccgtcttcgg
    19441 cagttccagc tcctacgcaa tattaagcgc agttaaccct ctccgcccgg agagggttag
    19501 tagttccagc gataacgctg caagtcgatt tgcccgcttc ccgataccat cacaccttct
    19561 gccagtaatg cctgtcgttg acgctgtaaa tccggtccgg ttagcgaaat tgtgccgtgg
    19621 cgattaacca cccgatgcca gggtaaggtg ctgccttcag ggagacgctt taacacaccg
    19681 cccacctggc gcgcggcgcg gggcgatcct gccagtttcg ccacatcacc gtaagtggtg
    19741 acatagcctt cgggaatagc ggctacgatt tgccagacgc gttggggaaa tgaatcttct
    19801 ttttccatct tttcttcctg aggtaatttt tcagcataat ctggaaaaac gcccgagtga
    19861 agtcgcattg cgcaagaaac cagcatccgg cacgcaatgg gttgcaatta gccggggcag
    19921 cagtgataat gcgcctgcgc gttggttctc aacgctctca atgggggctc tgttggttct
    19981 cccgcaacgc tactctgttt accaggtcag gtccggaagg aagcagccaa ggcagatgac
    20041 gcgtgtgccg ggatgtagct ggcagggccc ccacccattt ctgcctcccc ccgtttcgtc
    20101 aaaaaatccc aacatggcta aactttaacc acaactgacg tcgcaagaat tgtctggctg
    20161 cgcagtacgc ttcggaggta tgtctgatga agtatgttga tggttttgtg gttgccgttc
    20221 ctgccgataa aaaggatgcc tatcgggaaa tggctgctaa ggctgcgcca ttgtttaaag
    20281 agtttggcgc gcttcgtatt gtcgaatgct gggccagcga tgtaccggat ggcaaagtga
    20341 ccgattttcg tatggcggta aaagctgaag agaatgaaga ggttgtcttt agctggattg
    20401 aatacccctc aaaagaggtc cgcgacgctg ctaatcaaaa gatgatgtcg gacccacgga
    20461 tgaaagagtt cggcgagtcc atgccgtttg atggtaagcg aatgatttat ggcggattcg
    20521 agtcaatcat cgacgaatag gtagcgtgac gggctgtgcg gcgtgccgtc agccctacaa
    20581 ttcacaaatg ttgctcggcc cagcgtaaga aatcttcttt cggtaatgcc ttgctgtaga
    20641 gccagccctg accgtagtgc acgccatgct ggcgtaacca ctcttcttgc ttactggttt
    20701 cgattccctc cgctaccatt ttcagtttca gtgtttttgc catttcgatg atatgcggcg
    20761 tgacattttt atattccagc gcatcaacga aagatttatc gatcttcaga atgtcgacat
    20821 ccagatcctg taaataactt aaacttgaat accccgtacc aaaatcatca agataaattt
    20881 catggcccgc ctcccggtag cgagaaatta tcggggcgct ggttttcgga tcggcaaact
    20941 cgcgttcagt gagttcaagc gcgatctgtc tgggattaac ctgatagtga ttgatcattt
    21001 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    21061 tatgctgctg cggatgctga cgcagccagt cgcccatatc ttcgaagacg cttcttataa
    21121 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    21181 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    21241 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    21301 catcctgcag ccgatgatgt ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    21361 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    21421 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatt tccgggaagg
    21481 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagtc
    21541 tggtaataat tccctgagca atttcatcgc tgctggttat cacaacgtta tgggcattgc
    21601 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    21661 ggtcgatcat gacgacataa tgtgccgttc ccatggcaac catgtaacgg ataatgccta
    21721 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    21781 ggaaggtatc gggcgggctt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    21841 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    21901 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagtgca tctttccctt
    21961 gtatcgccac cttattggcc cgaatagcga cgcgggagga ataggtattc agctcttcaa
    22021 taaatgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    22081 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    22141 ccttattaac acagtgtaga gaaagtatat ccgattgtgg aaaaaggctg gcaggagata
    22201 aggaggtaga gccggtattt catcaagtga tgggtggaaa cgaacgttgt catttgacca
    22261 ggcgcagagc tttacgcctg gtatgtaact ctaacttaag atgttgattc ggcgcgagca
    22321 agggtaaaaa tatcgtaaaa agacaattca cctttacgga cgatcatttt ttgcagttgt
    22381 tctttttgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    22441 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    22501 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    22561 aatacaacca gtagccagac agagcctgaa agcgtttcgg actgcaacat caccgccagg
    22621 gtggcaaaaa aagcaacata cataccgata aacagccccg gatgtttacg gataaaactg
    22681 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat ttagagactc aattgtttcg
    22741 gtcaacaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    22801 aacaccctat tttaacgtgg gaatagtaga gggaaagtaa cagatttacc acaattttgc
    22861 ataacagttg cgaaaagctg gttacaattt tttaatgatt ctggctggat taccgcccac
    22921 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    22981 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    23041 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    23101 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    23161 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttgccgagaa aaatgttata
    23221 gccataatca cagcgaaacg ttgactcaat ataagcctct gtcacctgac cgaataaatc
    23281 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    23341 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    23401 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    23461 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    23521 gatcacatag ggtacgtctt catggcgaaa aagtataaaa ttcttaataa acagccggtt
    23581 atagctccga aagcgaatta tttattagcg aataaatttc catactgagg aagggatctt
    23641 gtcgtacagt ttattcatgg tcaattcggc gaggcggtga tctgcggctg agtaaaatac
    23701 cgccagttca ttatctgata attcgtattt atttttctcg ataacacgct ccagcgtgtc
    23761 aattgtctgg caacgacgta aacgcattaa ataatcggtt tttgttaaag gtttttcgga
    23821 catacttcta cccatggttg taataattct aacaagataa actcgcagga ttctctttcg
    23881 tcgcattgac aaaacaacgg aatagtcgat tacctgactt ccgccatttc tgaagatcct
    23941 gcatattaat accataactg ctgaacaaca taaaggtgtc atccagatat tcgtcgatct
    24001 gctcaatgag cttattgtct tcattatact taattttgta attaagtgcg aaggtcgcaa
    24061 tatgctcaat cagttcattc aactggaggt tgatcgccga ggttgggtcg ttaacccagc
    24121 catgattgct ttcttcaagg tttgcaagac agtcatgata cagggtttca cagagaaact
    24181 taagctgtgc gatatcatgt cttttgggtg agtattcatc cataacgcgt ccccttctta
    24241 gtggttgaac taacggacac ctttcgggat ggaaaaaact tactgacctg gacttgccct
    24301 ctcgtttgtt agcttaacta taagccactc tttgcaggtt ttcatcgcat tattacgaaa
    24361 aattacaatt aggaaggaat cgggacagga ctcatttttt catactagca ctaacccgca
    24421 gcaggtaaaa gcagtctgct cgaactattc ataacatttc aggattatcg ccagatgaaa
    24481 ttggtgacac taataccagg attgctctga atatgacgta ataaccgagg aatgaataaa
    24541 gaattaccgc aaataattaa gaatagcctc taaatgatta tggattatta aatctattag
    24601 cgctacttaa tataaattaa ctaacaatca gtatttttta tgaattttat gcgaggttaa
    24661 tactggtttt cgtatgagat cctgagttgg tggttcaatg actccttaat gttcgtcggt
    24721 tatgcataaa aaaggccgct tgcgcggcct tagtgattac acgttgtatc aatgatgatc
    24781 gacagtatgg ctgtgctcga tatcttcatt cttgcggcta aagcggcggc gaaccaccac
    24841 aaagaatacc ggaacgaaga agattgccag taccgttgcg gtcaccatcc cgcccattac
    24901 accggtacct actgcgttct gcgcgccgga accagcacca gtactgataa ccagcggcat
    24961 aacgccgagg ataaacgcca gcgaggtcat caggatcgga cgtaaacgca tccgcaccgc
    25021 atcaagcgtc gcttcaatca gacctttacc ttctttatcc atcaagtctt tggcgaattc
    25081 gacgataagt atcgcgttct tcgccgacaa cccaatggtt gtgagcaggc ctacctggaa
    25141 gtaaacgtca ttggtcaggc cacggaaggt ggcagccagc aacgcaccga taacccccag
    25201 cggaacgacc agcataacgg agaacggaat cgaccagctt tcatacagcg ccgccagaca
    25261 caggaacacg acaatcaacg aaatcgcgta cagtgaaggt gcctggttgc cggagagacg
    25321 ttcctgatag gacatccccg tccagtcata gccaacaccg gtaggcagtt tgctcgccag
    25381 ttgttccatc agctccattg cttcaccggt acttttaccc ggtgccgcct ggcctaagat
    25441 ttccatggat ggcaggccgt tgtaacgttc cagacgcggc gaaccgtact cccaacgaga
    25501 agaggagaac gccgagaacg gcaccatctg accatcagca gcacgaacat accagtcgcc
    25561 gatatcatcc ggcagcatac ggtatttcgc ttctgacatg acgtaaactt tcttcacacg
    25621 accgcggtcg ataaagtcgt tcacatagct accgccccat gcagcgccca gagtggtgtt
    25681 aatgtcgttg atagaaacac ccagcgcctg cgctttttcc tggtcgatat caatcttaaa
    25741 ctgcggggta tcttccagac cgtttggacg tacgctggtc aacatatcag ggtgcttcgc
    25801 tgcttctgca agcagctggt tacgcgcctg agtcagtttt tcgtgaccaa ggccagcctg
    25861 gtcaatcagc tcaaagtcaa agccggttgc agtacccagt tccacgattg cgggcaggtt
    25921 aaaggcgaaa accatcgcat ctttgatttg cgagaaagcg cgtgttgcac gcatggtaat
    25981 cgcttcaact ttgttttctt cacccggacg atcggcccag tccttcaagg aaacgaacgc
    26041 aataccggta ttctgaccac gtcccgcaaa gccgaagccg ttaacggcga acaccgactc
    26101 aacgttgttc ttttctttgg tcagatagta attcgttacc tcattgagca ctttctgcgt
    26161 acgttcctgc gttgcacctg ctggcagctg aaccatggtc ataaatacgc cctggtcttc
    26221 atctggcaag aaggagcttg gcagacgcac gaacagatag gccatgccga ccacgatgat
    26281 cagatacagc accaggtaac gccctgtact gcgcagaata ccgcctacgc tgtcggtgta
    26341 gtggtgcgtg ctcttctcga acatgcggtt aaaccagccg aagaagcctt ttttaccttc
    26401 cccgtgatcg cctttggcaa tcggtttcag catggtggca caaagagccg gagtcaggat
    26461 caacgccacc agtactgaca gcgccattgc tgaaacaatg gtaatagaga actgacgata
    26521 gatagcacca gtagaaccgc caaagaaggc catcggtacg aataccgccg acagtaccat
    26581 cgcgataccg accagagcgc cctgaatctg ccccatcgac ttacgggtag cttcttttgg
    26641 cggcaaacct tcttccgcca taacacgctc aacgttttct accacaacga tggcgtcatc
    26701 caccaacagg ccgatggcga gcaccatccc gaacattgtt agcgtgttta tcgagaagcc
    26761 aaaggcggca aggacggcaa aggtccccag caataccacc ggtacggcaa tggtcggaat
    26821 caacgtcgcg cggaagttct gcaagaacag atacataacc aggaacacga ggatgatcgc
    26881 ttcgaccagc gttttaacca cttcgtgaat agagattttc acgaacggcg tggtgtcgta
    26941 cgggtaaaca attttcagac ctgacgggaa gaacggttcc atcttcgcca gttcagcacg
    27001 gattgccgca gcggtatcca gcgcgtttgc accggtcgcc agcttgatcc ccagaccgga
    27061 agccggttgg ccgttaaact ctgcgatgat gtcgtagttc tcaccaccca gctcaatttt
    27121 cgccacatca cgcagcagca cgcgggaacc atcctgattc actttcagca ggattttgcc
    27181 aaactcttca gtagaggtca gacgcgtctg agcaataata gaggcgttaa gctgttggcc
    27241 tttcaccggc ggcgtaccac cgagctgacc cgccgcaacc tgggcgttct gcgctttgat
    27301 ggcggtaatg acatcaaccg gtgttagttg gaatttgttc agttcattcg ggttcatcca
    27361 gatacgcatc gcgtactgtg aaccgaacaa ctgaacgtca cccacgcccg acgtacggct
    27421 gatggcatct ttcatattcg ccgccacgta gtcggagata tcctcctgcg tcatggtgcc
    27481 atcggtgttg ataacgccga caaccatcag gaagctgctg gatgatttct caacgctcac
    27541 cccttgctgc tgtacttctt gcggcagcaa cggcatcgcc agctgcagtt tgttctgcac
    27601 ctgaacctgc gcgatatccg catcagtacc agactcaaag gtcagggtga tctgcacggt
    27661 acccgtggag tcactgttag aggacatgta catcaggtta tcgataccgt tcatattctg
    27721 ttcgataacc tgtgtcaccg tgtcctgcac tgttttcgca tcagcgccgg ggtaggaggc
    27781 ggagatcgtt actgccggcg gagcaatcgt aggatattgc gccaccggca gtttgaggat
    27841 cgccagcccc cctgccaaca tgatgataat ggcgatcacc cacgcaaaaa tcgggcgatc
    27901 gataaagaaa ttaggcatgt cttaacggct cctgtttaag ttaagacttg gactgttcag
    27961 gctgagcacc gcttgcggct tgctggttat tatcagcggt aacttcttgt gcttttacct
    28021 ggacaccagg acgcactttc tgcagcccac ttattactac gcgatcgcct gctttcagac
    28081 cttctgtcac cagccactta tcgccaatag cctggcttgc aacgatcgga cgggtttcca
    28141 ctttgtcatc cgcgccaacc accagtacgg tggcatcgcc acgcggcgta cgggttacgc
    28201 cctgttgcgg gactaaaata gcgtttggat taagcccttc ttccagacgt gcgcgcacga
    28261 acatacccgg cagcagagtg tgatccgggt tcgggaagat agcgcgtagg gtgatagacc
    28321 cggtggtctg atcaacggta acgtcagaga attccagcgt accgtcctgc gggaacttaa
    28381 tgccgtcact ggtgatcagc gacactttgg ctttgccgtt ctcttgtttc agcgtgccat
    28441 tcgccagttc ctgtttcagg cgcaggaagt cgttgctgga ctgggtcaca tcaacgtaga
    28501 tcggatcaag ttgctgcacg gttgccagcg cagtcgcctg accgttctgt accaatgcgc
    28561 cttccgtcac gttcgactta ccaatgcgac cgctaatcgg agaggtaact ttggtgtaag
    28621 ccagattgat ccgcgcagtt tcaacggcag ctttcgccgc agttaccgca gcattcgcct
    28681 gttgcgcatc agccagagcc tgatcgtact cttgcttact gatgtactga gtaccgagca
    28741 atttctgata acgattcacc gtcaattgcg cgatattggc tgcagcctgg gctttcgcca
    28801 gatcgccttt cgcgctgtcg tatgccgcct gataggtcgc aggatcaatc tgatagagag
    28861 agacacctgc ttcgatgtcg ctaccttctt tgaaattacg cttcaggata atcccgctaa
    28921 cttgaggacg aacttctgcg atccggtagg cactggtgcg acccggaagc tcggttgtga
    28981 tctgcagagg ttcagttttg actgttacta cgccaacggc gggcatctgc tggccacctt
    29041 gttgggcctg tttgtcgtca catcctgtta gggctaagct gcctgagagc atcagaacga
    29101 ccgccagagg cgtaaaccct ctgtttttgt tcatatgtaa acctcgagtg tccgatttca
    29161 aattggtcaa tggtcaaaag ttaataaacc cattgctgcg tttatattat cgtcgtgcta
    29221 tggtacatac attcacaaat gtatgtaaat ctaacgcctg taaattcacg aacatatggc
    29281 acgaaaaacc aaacaagaag cgcaagaaac gcgccaacac atcctcgatg tggctctacg
    29341 tcttttctca cagcaggggg tatcatccac ctcgctgggc gagattgcaa aagcagctgg
    29401 cgttacgcgc ggtgcaatct actggcattt taaagacaag tcggatttgt tcagtgagat
    29461 ctgggaactg tcagaatcca atattggtga actagagctt gagtatcagg caaaattccc
    29521 tggcgatcca ctctcagtat taagagagat attaattcat gttcttgaat ccacggtgac
    29581 agaagaacgg cgtcgattat tgatggagat tatattccac aaatgcgaat ttgtcggaga
    29641 aatggctgtt gtgcaacagg cacaacgtaa tctctgtctg gaaagttatg accgtataga
    29701 acaaacgtta aaacattgta ttgaagcgaa aatgttgcct gcggatttaa tgacgcgtcg
    29761 ggcagcaatt attatgcgcg gctatatttc cggcctgatg gaaaactggc tctttgcccc
    29821 gcaatctttt gatcttaaaa aagaagcccg cgattacgtc gccatcttac tggagatgta
    29881 tctcctgtgt cccacgcttc gtaaccctgc cattaccgaa taaccctgaa tctgactcca
    29941 ggatttttcc tggacatttt cgtcgttgct attctggttc actgcgtcgt gatattcttg
    30001 cggtttgact ttttcaggtc gttcttcagg ttcagaaacc ttcattcatc atgactatgt
    30061 tccagtatta caaacgatca cggcattttg ttttttcagc atttattgct tttgtttttg
    30121 tcttgttatg ccagaacacg gcgtttgcgc gggcgtcatc gaatggtgat ctgccgacaa
    30181 aagcggacct gcaggcgcaa cttgactcac taaataaaca aaaagatctt tctgctcagg
    30241 acaaactggt gcagcaggat ctgacagata cattagccac cctcgataaa atcgatcgcg
    30301 taaaagaaga gacagttcag ctacggcaaa aagtcgctga agcgccggaa aaaatgcgcc
    30361 aggcgaccgc ggcgttaaca gcacttagcg atgtcgataa cgacgaagaa acgcgcaaaa
    30421 ttctgagcac gctttcgttg cgccagctgg aaactcgcgt tgcccaggcg ctggacgatt
    30481 tgcaaaacgc acaaaacgat ctggcgtctt ataacagcca gctggtttcg ttacagacgc
    30541 agcccgaacg cgtgcaaaat gcgatgtata acgcttcgca gcagctgcaa caaattcgca
    30601 gtcgtctgga tgggactgat gtcggcgaga ctgccttacg tcccagccag aaagtgttaa
    30661 tgcaggctca gcaggctttg ctgaatgccg agattgatca acagcgtaaa agcctggaag
    30721 ggaacacagt cttacaggat accttgcaaa agcaacggga ttacgtgacg gcgaacagcg
    30781 cccgtctgga gcaccagttg cagctgttgc aagaagcagt aaacagcaag cgcctgactt
    30841 taaccgaaaa aacggcgcag gaagccgtct ccccggatga agccgcgcgt attcaggcta
    30901 atccgctggt gaagcaggaa ctggaaatta accagcagtt aagtcagcgt ctgattaccg
    30961 cgactgaaaa cggtaatcag ttgatgcagc aaaacattaa agtcaaaaac tggctggagc
    31021 gggcgctgca atcggaacgc aatattaaag agcagattgc cgtcctgaag ggcagcctgc
    31081 tgttgtctcg tatcctttac cagcaacaac aaacgctgcc ctcggcggat gaactggaaa
    31141 acatgaccaa ccgcatcgcg gatttgcgtc tcgaacagtt tgaagtcaac cagcagcgtg
    31201 atgcactctt ccagagcgat gcgttcgtca acaaactgga agaaggtcac gccaacgaag
    31261 tcaacagcga agttcacgat gcgttattgc aagtggttga tatgcgtcgc gaattgctgg
    31321 atcaactcaa caaacagttg ggtaatcagc tgatgatggc cattaacctg caaattaacc
    31381 agcagcagtt aatgagtgtg tcgaaaaacc tgaaatccat cctgactcag caaatctttt
    31441 gggtgaacag taaccgtcca atggactggg actggatcaa agcgttcccg caaagcctga
    31501 aagatgaatt taagtcgatg aaaatcacgg tgaactggga aaaagcctgg cctgccgttt
    31561 ttatcgcttt cctcgccggt ttgccgctgc tgttgattgc cgggctgatc cactggcgtc
    31621 tgggctggct gaaagcgtat caacaaaaac tggcttccgc tgtgggttcc ctgcgtaacg
    31681 acagccagct caacacacca aaagcgatcc ttatcgacct gatccgcgcg ctgccggtgt
    31741 gcctgattat tctcgcggtt ggtctgattc tgttgaccat gcaactcaac atcagcgaac
    31801 tgctatggtc gttcagcaaa aaactggcga ttttctggct ggtgtttggc ctgtgctgga
    31861 aggtactgga gaaaaatggc gttgccgtgc gtcacttcgg catgccggaa cagcagacca
    31921 gccactggcg tcggcaaatt gtccgcatca gtctcgcgtt gctgcctatc catttctggt
    31981 ctgtggtggc agaactttcc ccgctgcatc tgatggatga tgtgctgggg caggcgatga
    32041 ttttcttcaa cctgctgctg attgccttcc tggtatggcc gatgtgccgc gaaagctggc
    32101 gtgataaaga gtcgcacacc atgcgactgg tcaccattac cgtgctgtcg attatcccga
    32161 ttgcgttgat ggtgctgacc gccacgggtt atttctacac cacgctgcgt ctctccgggc
    32221 gttggattga aaccgtttat ttggtgatca tctggaacct gctgtaccag acagtactgc
    32281 gtggcttaag cgtagcggcg cggcgtatcg cctggcgtcg tgcgctggcg cgtcggcaga
    32341 atctggtgaa agagggcgca gaaggtgccg aaccgccgga agaacccacc attgcactgg
    32401 agcaagttaa ccagcagacg ctgcgtatta ccatgttgct gatgtttgcg ctgttcggtg
    32461 tcatgttctg ggcaatttgg tccgatttga tcaccgtgtt cagctatctc gacagcatca
    32521 cgctctggca ttacaacggc actgaagctg gcgctgcggt ggtgaaaaac gtcaccatgg
    32581 gcagtctgtt gtttgcgatt atcgcctcaa tggtggcctg ggcgttgatt cgcaacctgc
    32641 ctggtttgct ggaagtgctg gtgctctcgc gactgaatat gcgccagggc gcgtcgtatg
    32701 ccattactac catccttaac tacatcatta ttgccgttgg tgcgatgacg gtgttcggat
    32761 cgctgggcgt ctcttgggat aaactccagt ggctggcggc agcattatcc gtaggtcttg
    32821 gttttggttt acaagaaatt ttcggtaact tcgtctccgg tttgatcatt ctgttcgaac
    32881 gtccggtacg cattggcgat acggtaacca ttggtagctt ctcggggacg gtaagtaaga
    32941 ttcgtattcg tgcgacaacg attaccgatt tcgatcgcaa agaagtgatc atcccgaaca
    33001 aagcgtttgt taccgagcgt ctgatcaact ggtcgttgac tgacacgact acgcgtctgg
    33061 tgatccgtct cggcgtggcc tatggctccg atctggaaaa agtgcgtaaa gtgttactga
    33121 aggcggcgac tgagcaccca agggtgatgc acgaaccaat gccggaagtt ttctttacgg
    33181 cgtttggtgc cagcacgttg gatcatgagc tgcgtctgta tgtgcgtgaa ctgcgtgacc
    33241 gtagccgtac tgtcgatgag ctgaaccgta ctatcgatca gctgtgccgt gaaaacgata
    33301 tcaacattgc ctttaaccag cttgaagtgc atctgcacaa cgagaagggc gatgaggtga
    33361 cggaagtcaa acgcgactac aaaggcgacg atccgacacc agcggtaggg taaggacaat
    33421 aaagggcgac agtgatgccg ccctttattt tttactcgct ttgcgaagcc tcatcgctct
    33481 gtaacttctt ttcataatca cgcttcacaa tatccagcgc gcgcagcacg gtgcgggctg
    33541 ggatctgatt ttcttccagc agcacaatca aatcgacagc cagtttgaca tcgtcagggg
    33601 cattttccag tgacatactc tctccattgc tagcgggtta agcgcgctag cctgttttcg
    33661 attttttcca gcgcatggcg gcagcgtgcc aggcgcgctt cataggcttc cacttcacga
    33721 tgcagcgttt gctgttccac gagatcggtc acccgcgcca ggcgggctcg acgttcggct
    33781 accatctcac gtagccgccg ctcaaaatcc tgatgctgaa tacgtttacg ctgccagcgg
    33841 gcaattttcg gtggcgcact gtcccactcg cgtagcgacc aggctgtggc ttcacgcgct
    33901 atagcctcca gttgcgccgc cagatgttct gccagccagg ccacttgcgg cagctgttgc
    33961 tgctcaaccg catgacgaag cgcagccatg ttatcgcccg cctcatcgag acaagcctgc
    34021 agtgttgtcg cacgggtctg aaaaaggtgc ctgtcaaagc gggcacttag cgtggcaaat
    34081 tgcgctaccg gggcacaacg ctggcgcagc gtggcgagct gtccttccag tttttccagc
    34141 agcagggcgg ttttcatgat aagacctcaa tgaaaatgat aattgttatg ctaaagtagc
    34201 cactctgtaa gctgacaaca ctcaatatgc aacgaatcat tttaatcatc attggctggc
    34261 tggcggtagt gctgggtacg ctgggcgtgg tattaccggt attaccgacg acgccgttta
    34321 tcctgctggc ggcctggtgc tttgcccgtt cttccccgcg ctttcacgcc tggttgctgt
    34381 accgctcatg gtttggcagc tatctacgtt tctggcagaa acatcatgcg atgccgcgcg
    34441 gcgtcaaacc gcgggcgatt ttgcttattt tgctcacgtt tgcaatttct ctgtggttcg
    34501 tccagatgcc atgggtgcgc atcatgttgc tggtaattct cgcctgtttg cttttttata
    34561 tgtggcgaat tccggtgatt gatgaaaagc aagaaaagca ctgaagcaca acaatcgcag
    34621 ttgcaattat tgcgtacagc cagtacattc tggcgttttc gagcacaggc gcaggcggtc
    34681 aaaggttaaa caactgttac ttttgatacg tttaaaacgc gccgtgagta ccaccgtaac
    34741 aagcaggcat acacttatga ccgcgactgc acagcagctt gagtatctca aaaatagcat
    34801 caaaagcatt caggactacc caaaacccgg cattcttttc cgcgatgtca ccagcttact
    34861 ggaagacccg aaagcttacg ctctcagcat cgacttgctg gttgagcgtt acaaaaatgc
    34921 gggcattacc aaagttgtcg gcaccgaagc gcgtggcttc ttgtttggcg ctccggtagc
    34981 tctgggtctg ggcgttggct ttgtaccggt ccgtaaaccg ggcaaactgc cgcgtgaaac
    35041 catcagtgaa acttacgatc tggaatacgg caccgatcag ctggagatcc acgttgatgc
    35101 catcaaaccg ggcgacaaag ttctggtggt ggacgatctg ctggcaaccg gcggcactat
    35161 cgaagcgacc gttaaactga tccgtcgtct gggtggtgaa gtggctgacg ctgcgttcat
    35221 tatcaacctg ttcgatctcg gcggcgagca gcgtctcgaa aaacagggca ttaccagcta
    35281 cagccttgtt ccgttcccgg gccattaatt atcgccagtc ttgtgctgcc cacgctacgg
    35341 acagcacaag atgtgcattc agcctcgccg ttctgacggg gctgtgttag cattacccct
    35401 tcgtgaatcc accttccagc gtttcagagc ctgccaatga gttatcaggt cttagcccga
    35461 aaatggcgcc cacaaacctt tgctgacgtc gtcggccagg aacatgtgct gaccgcactg
    35521 gcgaacggct tgtcgttagg gcgtattcat catgcttatc ttttttccgg cacccgtggc
    35581 gtcggaaaaa cctctatcgc ccgactgctg gcgaaggggc taaactgcga aaccggcatt
    35641 accgcgacgc cgtgcggcgt gtgcgataac tgtcgtgaaa tcgagcaggg gcgctttgtc
    35701 gatctgattg aaatcgacgc cgcctcgcgc actaaagttg aagacactcg cgacctgctg
    35761 gataacgtcc agtacgctcc ggcgcgtggt cgtttcaaag tttatctgat cgacgaagtg
    35821 catatgctgt cgcgccacag ctttaatgca ctgttaaaaa cccttgaaga gccgccggag
    35881 cacgttaagt ttctgctggc gacgaccgat ccgcagaaat tgccggtgac gattttgtca
    35941 cgctgtctgc aatttcatct caaggcgctg gatgtcgagc aaattcgcca tcagcttgag
    36001 cacatcctca acgaagaaca tatcgctcac gagccgcggg cgctgcaatt gctggcgcgc
    36061 gccgctgaag gcagcctgcg agatgcctta agtctgaccg accaggcgat tgccagcggt
    36121 gacggccagg tttcaaccca ggcggtcagt gcgatgctgg gtacgcttga cgacgatcag
    36181 gcgctgtcgc tggttgaagc gatggtcgag gccaacggcg agcgcgtaat ggcgctgatt
    36241 aatgaagccg ctgcccgtgg tatcgagtgg gaagcgttgc tggtggaaat gctcggcctg
    36301 ttgcatcgta ttgcgatggt acaactttcg cctgctgcac ttggcaacga tatggccgcc
    36361 atcgagctgc ggatgcgtga actggcgcgc accataccgc cgacggatat tcagctttac
    36421 tatcagacgc tgttgattgg tcgcaaagaa ttaccgtatg cgccggaccg ccgtatgggc
    36481 gttgagatga cgctgcttcg cgcgctggcg ttccatccgc gtatgccact gcctgagcca
    36541 gaagtgccac gccagtcctt tgcgcccgtc gcgccaacgg cagtaatgac gccaacccag
    36601 gtgccgccgc aaccgcaatc agcgccgcag caggcaccga ctgtaccgct cccggaaacc
    36661 accagccagg tgctggcggc gcgccagcag ttgcagcgcg tgcagggagc aaccaaagca
    36721 aaaaagagtg aaccggcagc cgctacccgc gcgcggccgg tgaataacgc tgcgctggaa
    36781 agactggctt cggtcaccga tcgcgttcag gcgcgtccgg tgccatcggc gctggaaaaa
    36841 gcgccagcca aaaaagaagc gtatcgctgg aaggctacca ctccagtgat gcagcaaaaa
    36901 gaagtggtcg ccacgccgaa ggcgctaaaa aaagcgctgg aacatgaaaa aacgccggaa
    36961 ctggcggcga agctggcggc agaagccatt gagcgcgacc cgtgggcggc tcaggtgagt
    37021 caactttcgc taccaaaact ggtcgaacag gtggcgttaa atgcctggaa agaggagaac
    37081 ggcaacacgg tgtgtctgca tttgcgctcc tctcagcgtc atctgaataa cagcggcgca
    37141 cagcaaaaac tggctgaagc gttgagcacg ttaaaaggtt caacggttga actgactatc
    37201 gttgaagatg ataatcccgc ggtgcgtacg ccgctggaat ggcgtcaggc gatatacgaa
    37261 gaaaaacttg cgcaggcgcg cgagtccatt attgcggata ataatattca gaccctgcgt
    37321 cggttcttcg atgcggagct ggatgaagaa agtatccgcc ccatttgatc gtaagcacag
    37381 cttacgttcg tcatccttaa cgtgattgag agagaaacct atgtttggta aaggcggtct
    37441 gggtaacctg atgaagcaag cccagcagat gcaagaaaaa atgcagaaaa tgcaggaaga
    37501 gatcgcgcag ctggaagtga ccggcgaatc tggcgcaggt ctggtaaaag tgaccatcaa
    37561 cggtgcacac aactgccgtc gcgtagagat cgacccgagc ctgctggaag acgacaaaga
    37621 gatgctggaa gacctggtgg ctgcagcatt caacgacgcg gcacgtcgta tcgaagaaac
    37681 gcagaaagaa aaaatggcct ctgtatcctc cggaatgcag ttgccgcctg gctttaagat
    37741 gccgttctga tgcaaaccag cccgctgtta acacagctta tggaagcact gcgctgcctg
    37801 ccgggcgttg gcccgaagtc ggcgcagcgt atggcgttca cgctgcttca gcgcgatcgt
    37861 agcggcggga tgcgtctggc gcaggcgctc acccgggcga tgtcggaaat cggccactgc
    37921 gccgattgcc gcactttcac cgaacaggaa gtctgtaaca tctgttcgaa tccgcgtcgt
    37981 caggaaaacg gtcaaatctg cgtggtggag agtccggcgg acatctacgc cattgagcag
    38041 acggggcagt tttcaggccg ttattttgtg ttgatggggc acctgtcacc gctggacggc
    38101 atcggtccgg atgatatcgg gcttgatcgt ctggaacagc gtctggcaga ggaaaaaatc
    38161 actgaagtga tcctcgccac caaccccacg gttgaaggtg aagctaccgc taactacatt
    38221 gccgagcttt gcgcgcaata tgacgtggaa gccagccgaa tcgctcatgg cgtaccggtt
    38281 ggcggcgaac tggaaatggt cgatggcacc acgctgtcac actcccttgc cgggcgtcat
    38341 aagattcgtt tttaagcaaa cgagagcagg atcacctgct ctcgcttgaa attattcccc
    38401 cttgtcccca tctctcccac atcctgtttt taaccttaaa atggcattat tgaggtagac
    38461 ctacatgaaa ggacaagaaa ctcgtggttt tcagtcagaa gtgaaacagc ttctgcacct
    38521 gatgatccat tctctctatt ccaataaaga aatcttcctg cgtgagctta tctctaacgc
    38581 ctccgatgcg gcggacaagc tgcgtttccg cgcgctctct aacccggacc tgtacgaagg
    38641 tgatggcgaa ctgcgcgttc gtgtctcttt cgataaagac aagcgcacgc tgaccatctc
    38701 cgataacggc gtggggatga cccgcgacga agtgattgac catctgggga ctatcgctaa
    38761 atccggcacc aaatcattcc tcgaatccct gggttctgac caggcgaaag acagccagct
    38821 gatcggtcag tttggtgttg gtttctactc tgcttttatc gtggctgaca aagtgaccgt
    38881 gcgtactcgc gcggcaggcg aaaaaccaga aaacggcgtc ttctgggaat cggctggcga
    38941 aggtgaatac accgttgccg acatcactaa agaagatcgt ggtactgaaa tcaccctgca
    39001 tctgcgtgaa ggcgaagacg agttcctcga tgactggcgt gtgcgttcca tcatcagcaa
    39061 atattccgac catatcgcgc tgccggtaga gatcgaaaaa cgtgaagaga aagacggcga
    39121 aaccgttatc tcctgggaga aaatcaacaa agcgcaggcg ctgtggactc gtaacaagtc
    39181 ggaaatcacc gatgaagagt acaaagagtt ctacaaacac atcgcccacg actttaacga
    39241 tccgctgacc tggagccaca accgcgttga aggtaagcag gagtacacca gcctgctgta
    39301 catcccgtcc caggctccgt gggatatgtg gaaccgtgat cataaacacg gcctgaaact
    39361 gtatgttcag cgtgtgttca tcatggacga cgcagaacag ttcatgccga actatctgcg
    39421 cttcgtgcgt ggtctgattg actccagcga tctgccgctg aacgtttccc gtgaaatcct
    39481 ccaggacagt acggtaacgc gtaacctgcg caatgcgctg accaagcgtg tgctgcaaat
    39541 gctggaaaaa ctggcgaaag acgacgcgga aaaataccag accttctggc aacagtttgg
    39601 cctggtactg aaagaaggtc cggcggaaga tttcgctaac caggaagcga tcgccaaact
    39661 gctgcgtttt gcgtctactc ataccgattc ttctgcgcag accgtgtctc tggaagacta
    39721 cgtttcccgc atgaaagaag ggcaggagaa aatctactac atcaccgcag acagctatgc
    39781 ggcagcgaag agcagcccgc acctggaact gctgcgtaag aaaggcatcg aagttctgct
    39841 gctttccgac cgcatcgatg agtggatgat gaactatctg actgagttcg acggtaaacc
    39901 gttccagtcg gtgtctaaag ttgacgagtc gctggaaaaa ctggctgacg aagttgatga
    39961 gagcgcgaaa gaagcggaga aagcactgac tccgttcatc gaccgtgtga aagccctgct
    40021 cggcgagcgc gtgaaagatg tccgtctgac tcaccgtctg accgatacgc cagcgattgt
    40081 ctctaccgat gcggacgaaa tgagcactca gatggcgaaa ctgtttgccg cagcgggcca
    40141 gaaagtgccg gaagtgaaat acatcttcga actgaacccg gatcacgtac tggtgaaacg
    40201 tgcggcagat actgaagatg aagcgaagtt cagcgagtgg gtagaactgc tgctggatca
    40261 ggcgctgctg gcagaacgcg gcacgctgga agatccgaac ctgtttattc gtcgtatgaa
    40321 ccagctgctg gtttcctgat gtaatgccgg atgaccttcg tgtcatccgg catttttctt
    40381 ttcattatct gcactttccg caaattatct cgccattaac cgtttcagcc cctggtgcct
    40441 ttcttgaggc aatcgcctgt tggtggtatc gtttatcgct ttttcaaaaa attcgacaca
    40501 ttttaagggg attttcgcaa tgcgtatcat tctgcttggc gctccgggcg cggggaaagg
    40561 gactcaggct cagttcatca tggagaaata tggtattccg caaatctcca ctggcgatat
    40621 gctgcgtgct gcggtcaaat ctggctccga gctgggtaaa caagcaaaag acattatgga
    40681 tgctggcaaa ctggtcactg acgaactggt gatcgcgctg gttaaagagc gcattgctca
    40741 ggaagactgc cgtaatggtt tcctgttgga cggcttcccg cgtaccattc cgcaggcaga
    40801 cgcgatgaaa gaagcgggca tcaatgttga ttacgttctg gaattcgacg taccggacga
    40861 actgatcgtt gaccgtatcg tcggtcgccg cgttcacgcg ccgtctggtc gtgtttatca
    40921 cgttaaattc aatccgccga aagtagaagg caaagacgac gttaccggtg aagaactgac
    40981 tacccgtaaa gacgatcagg aagaaaccgt acgtaaacgt ctggttgaat accatcagat
    41041 gactgcaccg ctgatcggct actactccaa agaagcggaa gcgggtaaca ccaaatacgc
    41101 gaaagttgac ggcaccaagc cggttgctga agttcgcgct gatctggaaa aaatcctcgg
    41161 ctaatcaagc cgcagcagat ctgctttcgg gcaggtctgc tcatcctcct caggcaatac
    41221 ctctcacagc aattagttct tcttcttcac ttttccgcta caattatcaa caagttgaat
    41281 cgataagagg cggtaatgcg tcagactaaa accggtatcc tgctagcaaa cctgggtacg
    41341 cccgatgccc ccacacctga agcggtaaaa cgctatctga aacaattttt aagcgacaga
    41401 cgcgtggttg atacctcacg gttgttatgg tggccattgc tgcgcggcgt gattttgcca
    41461 ctgcgctcgc cgcgtgtggc gaagctgtat gcctctgtct ggatggaggg tggctcgccg
    41521 ctgatggttt acagccgcca gcaacagcag gcgctggcac aacgtttacc ggagacgccc
    41581 gtagcgctgg gaatgagcta cggctcgcca tcactggaaa gcgccgtaga tgaactcctg
    41641 gcagagcatg tagatcatat tgtggtactg ccgctttatc cgcaatactc ctgttcaacg
    41701 gtcggtgcgg tatgggatga actggcacgc attctggcgc gcaaacgtag cattccgggg
    41761 atatcgttta ttcgtgatta cgccgataac cacgattaca ttaatgcact ggcgaacagc
    41821 gtacgtgctt ctttcgctaa acatggcgaa ccggatctgc tgctgctctc ttatcatggc
    41881 attccccagc gttatgcaga tgaaggcgat gattatccgc aacgttgccg cacaacgact
    41941 cgcgaactgg cttccgcact ggggatggca ccggaaaaag tgatgatgac ctttcagtcg
    42001 cgctttgggc gggaaccctg gctgatgcct tataccgacg aaacgctgaa aatgctcgga
    42061 gaaaaaggcg taggtcatat tcaggtgatg tgcccgggct ttgctgcgga ttgtctggag
    42121 acgctggaag agattgccga gcaaaaccgt gaggtcttcc tcggtgcggg cgggaaaaaa
    42181 tatgaatata ttccggcgct taatgccacg ccggaacata tcgagatgat ggctaatctt
    42241 gttgccgcgt atcgctaaag ctgagcggta aagaactgag cgccgtcacg aagagcctcg
    42301 tcggcggttt tcatcatccg tgaataatgc aaaaaggcgt gcagcgtgcc tgggtagagt
    42361 ttgaactcac agggctgctg atgcgccgct aacgtctggt aaagcagacg gctgtcatcc
    42421 agcagcggat cgaactccgc cccggcaata aaacagggcg gaacttcgcg agtgagatca
    42481 ttattaaaca ggcagtaata cggcgactcg cggtccgcgt cgttgcttaa atacgcctct
    42541 tcatacatct gtaaatcctg ttgcgttaag ccatcccaga caccgcccaa cagacgacga
    42601 gtcacggaat cccgtaatcc gtaaagccca taccacagca aaacgcccgc aactttaccg
    42661 caatcgatct gtttatcacg caaccacaac gcactggcga gcgccaacat ggcacctgcg
    42721 gaatcaccgg caaagccaat gcgggacata ttgatttgat aatcttccgc ctgctggtgg
    42781 aaataacaac aggcagccac aatttcctct atcgcttgcg gaaaacacgc ttcaggtgaa
    42841 agggtgtaat caatgccaat caccgtacat tggctgtagc ttgccagcag gcgcatgatg
    42901 cgatcgtggg tatcaagatt gccgagaata aaaccgccgc catgcaaata aaatagcgtc
    42961 gccgggctat ctggctgcgg acaaaagaga cgtgtttcca cctgcccata ttttgttgga
    43021 accatgtaag ctctggttgc catttctgga gcgcccgcat tccagaatcg gcgctcaagc
    43081 gtgtaatact gtcgttgctc agcaatcgtt cccgttgcgg gccagggcgg taaatccggc
    43141 tgaagagtat tcacaacggt cttcatttca gcagaaataa ggtccagaac gggtaatttg
    43201 ttttccggct tcatgcaaaa actcctttca aattacgtca ttgtaaggaa ccactgccat
    43261 gaaaatgcga tcccgcctgc tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga
    43321 ttgtgttaac attcgccgct cagttaacca cccgtaaaaa caaccatgaa atttcccggt
    43381 aaacgtaaat ccaaacatta cttccctgta aatgcacgcg atccgctgct tcagcaattc
    43441 cagccagaaa acgaaaccag cgccgcctgg gtagtgggta tcgatcaaac gctggtcgat
    43501 attgaagcga aagtggatga cgaattcatt gagcgttatg gattaagcgc cgggcattca
    43561 ctggtgattg aggatgacgt agccgaagcg ctttatcagg aactaaaaca gaaaaacctg
    43621 attacccatc aatttgcggg tggcactatt ggtaacacca tgcacaacta ctcggtgctc
    43681 gcggatgacc gttcggtgct gctgggtgtg atgtgcagca atattgaaat tggcagctat
    43741 gcctatcgtt acctgtgtaa cacctccagc cgtaccgatc ttaactatct acaaggcgtg
    43801 gatggcccga ttggtcgttg ctttacgctg attggcgagt ccggggaacg tacctttgct
    43861 atcagtcctg gccacatgaa ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc
    43921 ggagcctcgg cactggttct cacctcttat ctggtgcgtt gcaagccggg tgaacccatg
    43981 ccggaagcaa ccatgaaagc cattgagtac gcgaagaaat ataacgtacc ggtggtgctg
    44041 acgctgggca ccaagtttgt cattgcagag aatccgcagt ggtggcagca attcctcaaa
    44101 gaccacgttt ctatccttgc gatgaacgaa gatgaagccg aagcgttgac cggagaaagc
    44161 gatccgttgt tagcatctga caaggcgctg gactgggttg atctggtgct gtgcaccgca
    44221 gggccaatcg gcttgtatat ggcgggcttt accgaagacg aagcgaaacg taaaacccag
    44281 catccgctgc tgccgggcgc tatagcggaa tttaaccagt atgagtttag ccgcgccatg
    44341 cgccacaagg attgccagaa tccgctgcgt gtctattcac acattgcgcc gtacatgggc
    44401 gggccggaaa aaatcatgaa cactaacgga gcaggggatg gcgcattggc agcgttgctg
    44461 catgacatta ccgccaacag ctaccaccgt agcaacgtac caaactccag caaacataaa
    44521 ttcacctggt taacttattc atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc
    44581 tatcaggtac tgaaccagca ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac
    44641 agcctggaag agtcttactg ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc
    44701 gttcacgccg catctggcat tttgctctca atgcctgatg cgacgcttg
//
LOCUS       DAPOZO010000033        44090 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPOZO010000033 DAPOZO010000000
VERSION     DAPOZO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.033"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            93..908
                     /gene="ybiV"
                     /locus_tag="ROI21_001963"
     CDS             93..908
                     /gene="ybiV"
                     /locus_tag="ROI21_001963"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDX5110883.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            1060..2325
                     /locus_tag="ROI21_001964"
     CDS             1060..2325
                     /locus_tag="ROI21_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDX5110884.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANVQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(2292..3452)
                     /locus_tag="ROI21_001965"
     CDS             complement(2292..3452)
                     /locus_tag="ROI21_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDX5110885.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            3690..4991
                     /locus_tag="ROI21_001966"
     CDS             3690..4991
                     /locus_tag="ROI21_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5110886.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSNLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHNPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            5006..7369
                     /locus_tag="ROI21_001967"
     CDS             5006..7369
                     /locus_tag="ROI21_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HDX5110887.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTLIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDVSFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPVEEKRTLLVNGVEGSEWMR"
     gene            complement(7549..9144)
                     /locus_tag="ROI21_001968"
     CDS             complement(7549..9144)
                     /locus_tag="ROI21_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDX5110888.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGALQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIDG"
     gene            9363..10283
                     /gene="ldtB"
                     /locus_tag="ROI21_001969"
     CDS             9363..10283
                     /gene="ldtB"
                     /locus_tag="ROI21_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDX5110889.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(10341..11459)
                     /locus_tag="ROI21_001970"
     CDS             complement(10341..11459)
                     /locus_tag="ROI21_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDX5110890.1"
                     /translation="MSLPFLRTLQGDPFFQLLILVGVGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGAQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLIQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(11456..11923)
                     /gene="mntR"
                     /locus_tag="ROI21_001971"
     CDS             complement(11456..11923)
                     /gene="mntR"
                     /locus_tag="ROI21_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDX5110891.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            12109..12237
                     /gene="mntS"
                     /locus_tag="ROI21_001972"
     CDS             12109..12237
                     /gene="mntS"
                     /locus_tag="ROI21_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDX5110892.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            12509..14092
                     /gene="opgE"
                     /locus_tag="ROI21_001973"
     CDS             12509..14092
                     /gene="opgE"
                     /locus_tag="ROI21_001973"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDX5110893.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAMLLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(14141..14656)
                     /gene="ompX"
                     /locus_tag="ROI21_001974"
     CDS             complement(14141..14656)
                     /gene="ompX"
                     /locus_tag="ROI21_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDX5110894.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(14709..14774)
                     /locus_tag="ROI21_001975"
     CDS             complement(14709..14774)
                     /locus_tag="ROI21_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110895.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            15009..15896
                     /gene="rhtA"
                     /locus_tag="ROI21_001976"
     CDS             15009..15896
                     /gene="rhtA"
                     /locus_tag="ROI21_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDX5110896.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            16195..16698
                     /gene="dps"
                     /locus_tag="ROI21_001977"
                     /gene_synonym="pexB"
     CDS             16195..16698
                     /gene="dps"
                     /locus_tag="ROI21_001977"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDX5110897.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAISEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            17102..17848
                     /gene="glnH"
                     /locus_tag="ROI21_001978"
     CDS             17102..17848
                     /gene="glnH"
                     /locus_tag="ROI21_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDX5110898.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            17987..18646
                     /gene="glnP"
                     /locus_tag="ROI21_001979"
     CDS             17987..18646
                     /gene="glnP"
                     /locus_tag="ROI21_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDX5110899.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            18643..19365
                     /gene="glnQ"
                     /locus_tag="ROI21_001980"
     CDS             18643..19365
                     /gene="glnQ"
                     /locus_tag="ROI21_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDX5110900.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            19482..21707
                     /gene="ybiO"
                     /locus_tag="ROI21_001981"
     CDS             19482..21707
                     /gene="ybiO"
                     /locus_tag="ROI21_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDX5110901.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLSAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(21704..22630)
                     /gene="rlmF"
                     /locus_tag="ROI21_001982"
     CDS             complement(21704..22630)
                     /gene="rlmF"
                     /locus_tag="ROI21_001982"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDX5110902.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            22906..23166
                     /locus_tag="ROI21_001983"
     CDS             22906..23166
                     /locus_tag="ROI21_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX5110903.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            23431..25713
                     /locus_tag="ROI21_001984"
     CDS             23431..25713
                     /locus_tag="ROI21_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDX5110904.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAARLNVMGE
                     KTHDAGRDKVKNERYGIAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANAKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGMAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            25755..26432
                     /gene="ybiX"
                     /locus_tag="ROI21_001985"
     CDS             25755..26432
                     /gene="ybiX"
                     /locus_tag="ROI21_001985"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDX5110905.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHLQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRIKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            26506..26772
                     /gene="ybiI"
                     /locus_tag="ROI21_001986"
     CDS             26506..26772
                     /gene="ybiI"
                     /locus_tag="ROI21_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDX5110906.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            27037..27297
                     /locus_tag="ROI21_001987"
     CDS             27037..27297
                     /locus_tag="ROI21_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX5110907.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(27436..28521)
                     /gene="hcxB"
                     /locus_tag="ROI21_001988"
     CDS             complement(27436..28521)
                     /gene="hcxB"
                     /locus_tag="ROI21_001988"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDX5110908.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDKFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKSILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLTS"
     gene            complement(28662..29624)
                     /gene="ybiB"
                     /locus_tag="ROI21_001989"
     CDS             complement(28662..29624)
                     /gene="ybiB"
                     /locus_tag="ROI21_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDX5110909.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDHEGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(29652..31802)
                     /gene="dinG"
                     /locus_tag="ROI21_001990"
     CDS             complement(29652..31802)
                     /gene="dinG"
                     /locus_tag="ROI21_001990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDX5110910.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIATLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            complement(31973..32254)
                     /locus_tag="ROI21_001991"
     CDS             complement(31973..32254)
                     /locus_tag="ROI21_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDX5110911.1"
                     /translation="MRLEWKPMSLSDREQIMEYISKDNPLAAIALDNQFEAIAEQACE
                     RPEMYRAGRVAGTHEAVVRPHYVLVYQVEGDTLSVLRVLHTALQWPPAE"
     gene            complement(32254..32694)
                     /locus_tag="ROI21_001992"
     CDS             complement(32254..32694)
                     /locus_tag="ROI21_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110912.1"
                     /translation="MAPETIDHSTLHKLVESGVVDAAHVIGTQGGWSLTVKYGHTERP
                     LAAQRSRQIRLFKRLETVVNYLKDVGIARFEVDASNYDPDGQKKTTRPDRAEALKRAH
                     EAAAYDAWFREQVQAAIDDPRPALSHEEAKSLFAARKKALLKGD"
     gene            complement(32851..34215)
                     /gene="rhlE"
                     /locus_tag="ROI21_001993"
     CDS             complement(32851..34215)
                     /gene="rhlE"
                     /locus_tag="ROI21_001993"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDX5110913.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRAEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            34444..35115
                     /gene="cecR"
                     /locus_tag="ROI21_001994"
     CDS             34444..35115
                     /gene="cecR"
                     /locus_tag="ROI21_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDX5110914.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAISELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            35118..36113
                     /gene="hlyD"
                     /locus_tag="ROI21_001995"
     CDS             35118..36113
                     /gene="hlyD"
                     /locus_tag="ROI21_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDX5110915.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGCVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            36106..37842
                     /locus_tag="ROI21_001996"
     CDS             36106..37842
                     /locus_tag="ROI21_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5110916.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHANGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHGNE"
     gene            37835..38968
                     /locus_tag="ROI21_001997"
     CDS             37835..38968
                     /locus_tag="ROI21_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5110917.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPEVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            38979..40085
                     /locus_tag="ROI21_001998"
     CDS             38979..40085
                     /locus_tag="ROI21_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5110918.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(40047..40457)
                     /locus_tag="ROI21_001999"
     CDS             complement(40047..40457)
                     /locus_tag="ROI21_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDX5110919.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            40590..41351
                     /locus_tag="ROI21_002000"
     CDS             40590..41351
                     /locus_tag="ROI21_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDX5110920.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            41348..42589
                     /gene="clsB"
                     /locus_tag="ROI21_002001"
     CDS             41348..42589
                     /gene="clsB"
                     /locus_tag="ROI21_002001"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDX5110921.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDAHIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            42589..43545
                     /locus_tag="ROI21_002002"
     CDS             42589..43545
                     /locus_tag="ROI21_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDX5110922.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(43581..43970)
                     /locus_tag="ROI21_002003"
     CDS             complement(43581..43970)
                     /locus_tag="ROI21_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110923.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGMVATAGLVLVIIMN
                     AALMSERPVWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
BASE COUNT        10489 a        11774 c        11270 g        10557 t
ORIGIN      
        1 tcagcctcgc tttcattcga aatataattt gtgctctgcg tcacattgtt attacactgt
       61 tacggtttca ttttcgagcc aggagcacac ctatgagcgt aaaagttatc gtcacagaca
      121 tggacggtac ttttcttaac gacgccaaaa cgtacaacca accacgtttt atggcgcaat
      181 atcaggaact gaaaaagcgc ggcattgagt tcgttgtcgc tagcggtaat cagtattacc
      241 agcttatttc gttctttcct gagctaaagg atgagatctc ttttgtcgcg gaaaacggcg
      301 cactggttta cgaacatggc aagcagttgt tccacggcga actgacccga catgaatcgc
      361 ggattgtgat tggcgagttg ctaaaagata agcaactcaa ttttgtcgcc tgcggtctgc
      421 aaagtgcata tgtcagcgaa aatgcccctg aagcatttgt cgcactgatg gcaaaacact
      481 accatcgcct gaaacctgta aaagattatc aggagattga cgacgtactg ttcaagtttt
      541 cgctcaacct gccggatgaa caaatcccgt tagtgatcga caaactgcac atagcgctgg
      601 atggcattat gaaacccgtc accagtggtt ttggctttat cgacctgatt attcctggcc
      661 tgcataaagc gaatggtatt tcgcggttac tgaaacgctg ggatctgtcg ccgcaaaatg
      721 tggtagcgat tggcgacagc ggtaacgatg cggagatgct gaaaatggcg cgttattcct
      781 ttgcgatggg caatgctgcg gaaaacatta aacaaatcgc ccgttacgct accgatgata
      841 ataatcatga aggcgcgctg aacgtgattc aggctgtgct ggataacaca tcccctttta
      901 acagctgacc tctctaccgg agccatgcgc tccggttttc ccttcttttt acatcctgta
      961 catcacatca aatcattaaa cttgcattaa cttatttata acttatattg tcacatgcaa
     1021 atcattatag tttcgaatga aagttaatgc gaggtggtta tggcttctac ttttaccagc
     1081 gacacattgc ctgccgatca caaagcagct atccgtcaga tgaagcacgc gctgcgggcg
     1141 cagcttggcg acgtccagca gatctttaat cagctaagcg atgacattgc cacgcgagtg
     1201 gctgaaatca acgcactcaa agcacagggc gatgccgtct ggccggtgct gtcttatgcc
     1261 gatatcaaag caggtcatgt tactgcagag cagcgcgaac agattaaacg tcgcggttgt
     1321 gcggtgataa aaggccattt cccccgcgaa caagcgctag gctgggatca gtcgatgctg
     1381 gactatctgg accgcaaccg ctttgacgag gtctacaaag gccccggcga taatttcttc
     1441 gggacgctca gcgcttcacg tcccgagatt taccccatct actggtcgca ggcgcaaatg
     1501 caggcccgcc agagtgaaga aatggcgaat gtgcagtcgt ttctcaatcg tctgtggaca
     1561 tttgaaagtg atggaaagca atggtttaac ccggatgtga gcgtcatcta ccctgaccgt
     1621 atccgccgcc gtccgcccgg aacgacctcc aaaggtcttg gagcgcatac cgactccggg
     1681 gcgctggaac gctggctgct tccagcgtat cagcacgttt tcgccaacgt ctttaatggc
     1741 aatctggcga aatacgatcc ctggcatgcg gcacatcgta cggaagttga agagtacacg
     1801 gtggacaaca ccaccaaatg ttccgtgttt cggacattcc agggctggac agcgctctct
     1861 gatatgctgc ctggtcaggg gctgctgcac gtcgtgccca ttcctgaagc catggcgtac
     1921 gtactgttac gtccgctgct tgatgatgtg ccggaggatg aactgtgcgg cgtagcgccc
     1981 ggaagagtat tgccggtatc agagcaatgg catccactgt tgattgaggc gttaaccagc
     2041 attccaaaac tcgaagccgg agactccgtc tggtggcact gcgacgtcat ccattccgtt
     2101 gcccccgttg aaaatcaaca gggctggggc aacgtgatgt acattcctgc ggcaccgatg
     2161 tgcgagaaaa atcttgccta cgcgcacaag gtgaaggccg cactggaaaa aggcgcatcg
     2221 ccgggcgact tcccgcgcga ggactatgaa acaaactggg aaggacgctt tacgcttgcc
     2281 gacctcaaca ttcacggtaa gcgagcgttg ggaatcgaca gctgagtgtc gtacagccct
     2341 tcccgttcca cggcggtgtt cccttttcct gggcgaaccc gccgcactga ctcccgcacc
     2401 gccaatcgtc cgtgcagcag cagcgagccc accggagcat ccgggcgaat cagccgctgt
     2461 tgcaacattt gcaccgcttc attgcctagc tcatcgcgtg gcacatgcac agccgtcagg
     2521 ggaacatcct cgattgccgc aaggttaaag ccgtcgatac tcatcaccga gacatcctgt
     2581 ggtacgcgca gaccacgacg atgtagcgca tttaccgttc ccgccgccat aaaatccccg
     2641 ccgaccagaa acgccgtcgg caacgtcttg ccattggcgg catccagcca cgctccgacc
     2701 ttttcttccg cttctttagc gctaaaactg ctgatattca gcaaatgacg gtcatggtga
     2761 aaggccagat tatgctgacg ccatgattct ttaatccctg caaaccgctg ctccatagtg
     2821 tagcggcgca ggcacatcac attcatcacc tcccggtgac ccatttcaaa cagatagcgg
     2881 gcggcgaacg cgccaattaa ccgatggtcc ggcgcaatcg ccgggaggcg cattcgttca
     2941 tcacggcagt tgatcagcac acaaggctta gcgagatcgg cggccagatc atggatgtgc
     3001 ggatcgtcaa tccccagcag gatcgccgcc tgcgtatctg cttcattcat ccgcgagaga
     3061 aatagcgcgg gatcgctgtc aaactcctcc agcgcgcagt aacgcagccg cacttcatgc
     3121 ggcgataacg ctttgctgat gctctggatc acacggtaat aaaagatatc agatcgttgg
     3181 tcaaaagcgc gttgcggcgc gaaaataatc aggttgttca gcaacatacg acctgctgcc
     3241 atgccatcca tcacgcccaa ctcacgcgcg caggccagca cctgttcgcg agcgcgtgca
     3301 ctggtattcg ctttgccagc cagcacccgc gaaaccgtac tcggcgatag cccggtacgt
     3361 gcggcgatat cggcgatttt cagcttcttg tccattttgt gatctgcctc taattcggaa
     3421 atgacagaat tttcatgagc aaaaaagccc attctgtctg gcattaaatc catttcagta
     3481 atgtatatca gtcactcatg gaaaaacgtg cacaaaaccc gctttctttc gcttttaggg
     3541 tctcttactg tgaattggta gaacaacttc catacaaaaa taatggaaaa acgaacaata
     3601 aagactttat ttatcaaaaa gttaaaagtg gtgtgacaac tccattctga cgttgccaca
     3661 caaaccctat accattctgt ggagtgcata tgagtcaggg tatcaataac gatatgactg
     3721 ccagtaaacc gcgccatatc gtcaaaaatc tgcgttggtg gatactggtc ctttttttgc
     3781 tcggtgttac tgtcaactac atcacgcgta actcgctggg tattctcgcc cccgaactga
     3841 aaagcaacct ggggattacc accgaacaat attcctggat tgtaggtgct ttccagctcg
     3901 cctataccct attccaaccg ttgtgtggat ggctgattga cgtcattggc ctcaaactgg
     3961 gctttatgat ctgcgcggga ctgtgggcgc tggcctgtct gatgcatgcg ggggcgggaa
     4021 actgggtaca tctggcgctg ttgcgcttct ttatgggcgg tgcggaagcc gcggcaaccc
     4081 cagcaaacgc caaaaccatc ggtgaatggt ttccaaaatc agaacgcccg atcgccgcag
     4141 gctgggcagg cgtcggtttt tccatcggtg ccatgctggc cccaccgatt atctattttg
     4201 cccacgcttc attcggctgg cagggtgcat ttatgttcac tggcatcctg gcgatgctgt
     4261 gggtggtgct gtggtgggcg ttctatcaca acccggataa gcatcctaac ctcagcaaag
     4321 ccgagctgga ctttatccgc caggataacg aagcgccccc tgtcaagtta ccctttctga
     4381 ccgcactgaa aaccgtaggc aaaaataaac gcttttacgg tatcgctatc ccggccttta
     4441 tggcagaacc ggcgtgggcg gtgatgagtt tctgggttcc gctatacctg tccaaaacct
     4501 atggcatgga cctgaagcag atcgcattat tcgcctggtt gccgttcctc gccgctgatc
     4561 ttggcagtat tgccagcggc tatctgacca aactttacac ccgcctgttt gggctgagtc
     4621 gtgtgaactc cgttgtcgca tcatcggtca gtggcgcatt cctgatggtt tcgctggcgg
     4681 tgatggcctt taccaataac ccgtatatcg ccatcgtcct tatttccatc ggcggcttcg
     4741 gtcaccagat catctcctgc atgctgagcg cactggtggt ggaatccttc gacaaaggtc
     4801 agatggccac tgttaacggc atgcgcggtt ctgccgcgtg gatcgcaagt ttcctgttct
     4861 ccctgattat cggggttacc gctgacaaaa tcggctttaa ccccctgttt atcgccatgg
     4921 gcttctttga cctgattggt gctttcttcc tggtcacctt tattgctgaa cgtcgcgcaa
     4981 aacgcgcctg agagtggatg taaacatgaa aacgctgaaa aactggaccc tgcacaaaca
     5041 gtctgcccac cacgttgaac tgctggtcga tggccagcat atcctgtgcc tgtacgtgct
     5101 ggaagaaaat ctgttccgcg tgctgttaaa acgcaaaggc gagctggcgc tggatcgcac
     5161 ctggagcatt gctccggcgc aggatgtgcc atgggaaggg cgcagccgcg aagatctcag
     5221 cggctttagc ctgcccgcgt ggacattgat ccagcacgac gacaccgtgg ttatcgccac
     5281 cgagtcactg cgtgccaccg ttcaccagcc gctgtggctg gagtggcact atcgtaacaa
     5341 cgaaggcgaa tggctgccgt tggtgaatga ccgcccgacc agcgcttatc tgctgaatgc
     5401 ccacggcgac ggcgtggcgc actatcagag ccgtcgtaaa gatgaacgct tttatggcct
     5461 cggagataaa gccggcgacc tgcaacgcaa cggtaaacgt tacgagatgc gtaacctcga
     5521 cgcgatgggc tacaacgccg tgagtactga cccgttgtac aaacacattc cattcaccat
     5581 cacccgccgc gatgacgtta gcttcggcct gttttatgac aacctcagca gctgctggct
     5641 ggatctgggc aacgagatcg acaactacca caccgcctat cgccgctggc aggcggaagc
     5701 gggtgatatc gactactacc tgtttaccgg cgaacgcgta cttgatatca ccaaagcctt
     5761 cgtacgcctg accggcaaaa ccctgttcgg accgaaatgg agcctgggct acagcggttc
     5821 aactatgcac tacaccgatg cgccggacgc acaaaatcag ttgatgaact ttatccgcct
     5881 gtgtgaagaa catgcgattc cctgcgattc gttccagctc tcttccggtt atacctcaat
     5941 taacggcaag cgctatgtgt tcaactggaa ttacgacaaa gtgccgcagc cgaaagtgat
     6001 gagccaggca tttcacaatg ctggtttgca cttggccgcc aatatcaagc cgtgcctgtt
     6061 gcaggatcac ccacgctata acgaagtggc agaaagagga ctgttcatcc gtgattcaga
     6121 aacagatgaa ccagaacgtt ccagcttctg ggatgacgaa ggttctaacc tcgactttac
     6181 taatccgcag accatccagt ggtggcagga aggcgtgacc acacagcttc tggagatggg
     6241 tatcgactcc acctggaacg acaacaacga attcgaagta tgggacggtg aagcacgctg
     6301 tcacggcttt ggtcagacga tcgcgatcaa acatattcgc ccggtgatgc cactgctgat
     6361 gatgcgagcc tcgatggaag cccagcaacg tttcgctccg gaaaaacgtc cgtacttgat
     6421 ctcccgctcc ggctgcgcgg gaatgcagcg ctatgtgcag acctggagcg gcgataaccg
     6481 cactagctgg gacactctgc gctacaacac ccgcatgggt ctggggatga gcctctccgg
     6541 gctgtataac ctcggtcacg acgtcggcgg tttctccggt gataagccgg acccggaact
     6601 gtttgtacgc tgggtacaaa acggcgtgat gcatccgcgc tttacgattc actcgtggaa
     6661 cgatgaccat accgtaaacg aaccgtggat gtatccgggc gtcactccgg cgatccgcag
     6721 cgctattgag ttgcgctatc gcctgctgcc ctacttttat accctgatgt ggcaggcata
     6781 cgccgatgat gaaccaatgc tacgtccgac tttccttgac cacgagcacg acgttcagac
     6841 ttttgaagag tgtgatgact tcctgcttgg tcgcgatatt ttggttgcca gcgtcgtaga
     6901 gcaaggcgag cgccagcgcc gtgtatggct gccagataac gaaactggct ggtacgattt
     6961 ctacaacggc gaatggttca gtggcggtca gtggatcacc ctcgacgcac cgctggagaa
     7021 actgccgctg atggtgcgtg ccggggctgg tctaccgctc agcaaacgca tcacctatgt
     7081 gagcgcagaa caagacgata cccgcgaact gaaactgttc ccgctgaaag gtgtgggcac
     7141 aacgtccggc ctgctgtttg aagacgatgg cgaaagctgg ggctaccaaa ctggtaatgc
     7201 gctttgggtg gaatgggaaa tggtttgcga tggggcgacc atcaacctga aagtcaacgc
     7261 gcgcggggat tatcgacctg catggagtgc actgaaagtg tcattaccag tggaagaaaa
     7321 acgcacgctg ctggtcaatg gtgttgaagg gagtgaatgg atgcgttaaa tcattaccgg
     7381 ggccgggagg cgactgcgcc ttacccggtc cagccatcga atcctttacc tcagtatttc
     7441 acgctgcagg aaggcagcaa acctgtaaac cccgggagct taaataagta agtgaccggg
     7501 gtgaacaggt gaagccaatg catctgccgc atgaaagacg gcggggaatt aaccgtcgat
     7561 ccctttacta cgcagataat cttcgtaatt accgctaaag tcgatcacgc gttccggggt
     7621 gatttccaga atgcgggtcg ccagggagct tacaaactca cggtcgtgag aaacaaagat
     7681 cagcgtgccc tgatacagtt ccagcgccat gttcagcgac tcaatggatt ccatatccag
     7741 gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta acttaccaaa
     7801 cagcatccgc cctttttctc caccggaaag cactttcgct ggctttttga tgtcgtcctg
     7861 gctgaacagc aaacgaccga gaatgctgcg taccgcctgc tcgtcatcgc cttcctgctt
     7921 ccactggctc atccattcga acacggtcag atcattttcg aactcatatt catggtcctg
     7981 ggcgtagtaa ccaatgcgtg cgttttcaga ccattttacg gtgccgctgt ccggttgcag
     8041 agcgcccacc agcgttttca gcagcgttga tttaccgacg ccgttggtac ccagtaccgc
     8101 aagtttttca ccgacctcca gcagcaggtt gagattttta aacagcggac cgttatcaaa
     8161 ccctttggtc agaccttcca cttccagcgc gttacggaac agtttcttat cctgttcaaa
     8221 acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga ttttatcaat
     8281 ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcactaa agcggctaac
     8341 gaaagattgc agctcagcaa tctgcgcctt cttcttggcg ttatcagcca gcagacgttc
     8401 acgcgcctgg gtcgccgccg tcatgtactc atcgtagttg cccggataaa cgcgcagctc
     8461 gccgtaatcc agatccgcca tgtgggtaca aaccatgtta agaaagtgac ggtcgtgcga
     8521 gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc gaatggtgtc
     8581 gatgtcgagg ttgttggtcg gttcgtcgag caggagaatg tccggatcag caaacagcgc
     8641 ctgcgccagc agcacacgca gcttccagcc aggagcaact tcactcatcg ggccgtagtg
     8701 ctgttccact ggaattccca cgccaagcaa cagttcaccg gcgcgagctt ccgcagagta
     8761 accgtccatt tcgccgtatt tcacttccag atcggccact ttatagccgt cttcctcact
     8821 catttccggc aaagcataga tgcggtcacg ctcctgcttc acttcccaca actctttatg
     8881 ccccatgatc accgtatcca gcacagtgaa ctcttcaaag gcaaactgat cctgacgcag
     8941 cttaccaatg cgctcgttgg gatcgaggga aacgttaccc aacgtcggct ctaagtcgcc
     9001 gccgaggatc ttcataaagg tggatttacc actaccgttc gcgccaatca ggccgtaacg
     9061 gttgccgccg ccaaatttga cggaaatgtt ttcaaacaac ggcttactgc cgaactgcat
     9121 ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac aaaccgcgca
     9181 ttatgccata agtctgaaag aagatcctgc tcctcgctta aggtctacac tcaagccaaa
     9241 ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg aacttataat
     9301 gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa atctcgctta
     9361 acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc ggcttttgca
     9421 gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg gttggtcaga
     9481 atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt gccgccgagt
     9541 accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc ttcctgccga
     9601 aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc gttcatgaag
     9661 gcatcgtcat taacagtgca gagatgcgtc tgtattacta tccgaaaggg accaacaccg
     9721 ttatcgtgct gccgatcggc attggtcagt taggcaaaga cacgcctatc aactggacca
     9781 ccaaagttga gcgtaagaaa gcaggcccga cctggacgcc gaccgccaaa atgcacgcag
     9841 agtaccgcgc tgcgggcgaa ccgcttccgg cggtcgttcc ggctggtccg gataacccga
     9901 tggggctgta tgcactctac atcggtcgcc tgtatgctat ccatggcacc aacgccaact
     9961 tcggtatcgg cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa gacatcaaat
    10021 tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag ccggtaaaag
    10081 cgaccaccga gccagacggc agccgttata ttgaagtcca taatccgctg tctaccaccg
    10141 aagcccagtt tgaaggtcag gaaattgtgc caattaccct gacgaagagc gtgcagacag
    10201 tgaccggtca gccagatgtt gatcaggttg ttctggatga agcgatcaaa aatcgttccg
    10261 ggatgccggt tcgtctgaat taatcgtcaa aacgtaaaac aaaggcggac tcatcatccg
    10321 ccttttttat ttgccagacc ctaccaggcc gggatcataa ccaacaaaac gtatcccacc
    10381 agcgccgccc acaacagcat cggtattgaa tagagatgga aacgccacca gatacggcga
    10441 tcgttcgcca tccgtaacgc aatcaaattt gccagcgatc cgggtaataa accaaagccc
    10501 cccacgttta ccgcccatgc cagcagcaac gacggcggca catagttcag caacaatatg
    10561 gtactcggca cgttactgat cacctgcgat aaaccgattg ccgttaacca taacccgggt
    10621 tcagataaat gactcacgtt acccaacacg ccttgcaacg ctggaagctg gatcagtaaa
    10681 tggacgtcga taaacatcgc cataaacacc agcagcagcg tccagtccac actcaacacc
    10741 acgcgacgtg ccagcagcgc aaagcccgcc gccacaatca ccagtcccca cagctcttgt
    10801 tttaactcca gcgccgtcag aaagacgata tacagcccca gacaactcca caccagccgc
    10861 ggtttccact ccggcgtttg cgcccccgta tggtattgca gcgcctttcc agggaaacaa
    10921 caccagcaca ggagcaggag cgtcagcatc attgcgccag ccagtggtgc catttgggca
    10981 ataaatccgg caaacgaaag accagaacgt ccccagataa gaatattttg cgggttgcca
    11041 attggcgtca gtagcgaacc agcgttgact gccagcgctt caaaaataat cagccgatta
    11101 accgggatct cacacagtct ttttagcgtg atagtcagcg gaacaacaat aaacagcgcg
    11161 acatcgttgg tcagaaaggt agaaagcagc gccgccgcca gcaccataaa catcgccagc
    11221 cgacgctccg tagcaaagcg gcgcaccatt ttgcgcccca gcacatcaaa ataaccgctt
    11281 agctccacac ctttagtcag cagcatcagg ccgcttaagg tgatgatggt gtgccagtcg
    11341 atagcagcag gccaggattt cggtgcaaag ggaacaaaaa aacttaatcc gacgccaaca
    11401 agaattaata actgaaaaaa aggatcgcct tgcagcgtgc gtaaaaaagg caggctcatt
    11461 tggcaccgtg tttctgggta aacaaacgaa aggcatccag cgtctcttca ctaacatggt
    11521 gctccatgcc ttccgcgtcg cgacgggcaa tttccggact gacgcccaac accaacaaga
    11581 aattttcgac tatctgatgt cgctcgcggc tttcctgcgc cagcttctct ccttctgccg
    11641 ttaaaaacac gccacgccag gggatcattt caatcagccc catagttgcc agtcgcttaa
    11701 gcattttagc caccgtcggt tgcgaaactc ccaaacgagc agccatgtct acctgacgag
    11761 cttcccccac ttccctgatc aagtcagaaa tcagctcaac gtaatcatca ataagctcgc
    11821 gcctatgcgc ctctctgacc tggcggaacc cttcaacgtg ctcttccacg ttcactaact
    11881 gcgtcacttt ttttgctgtt ggcgtacctg cgcgacgact cattgtgctt cctcattatg
    11941 gtgacgcata cctagcgttc tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta
    12001 caaaaaatta acgttttagt aatagctata taatatagcc tgtgctatat ttgtatgtaa
    12061 tgcaatcatc cctcaaggat cgacgggatt agcaagtcag gaggtcttat gaatgagttc
    12121 aagaggtgta tgcgcgtgtt tagtcattct ccctttaaag tacggttaat gctgctctct
    12181 atgttgtgcg atatggtcaa caacaaaccg cagcaagata aaccttccga taaatagcgg
    12241 cgtcgcggta cgccgcttca ctcctgcttt tatgcaggca caacgcgttt tggtctgaaa
    12301 aaccccactt tttgtcggat ttgcaatctc cttcgcaaaa gatttgttcg tcagtagttg
    12361 acctgaacgg cggctcgctc tatcttcttg cagccctgcg tatattgcgg ctcgcggatg
    12421 cggacccctt tcccctcttc acgcactctt gcaggtattg acccttgacg ccagggtaag
    12481 cacatggcgt ttgttacgat agtggcatat gaatttaacc ctcaaagaat cgcttgttac
    12541 ccgtagccgg gtatttagcc cgtggactgc gttctacttt ttacagtcgc tattaattaa
    12601 cctcggctta ggttatccct tcagtttgct ctacaccgct gcgtttacgg ctatgttgct
    12661 tttgctatgg cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt
    12721 ggcggcttgt tatttccctt ttgctcaggc ctacggcgcg cctaatttca atacattgct
    12781 ggcattgcac tccaccaata tggaagagtc gaccgaaatc ctgacgattt ttccgtggta
    12841 cagctacctg gtcggcttat ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa
    12901 agagaatgaa aaagcgcgct ggaatacctt cgacagcctg tgtctggtat tcagtgtggc
    12961 gacatttttt gttgctcccg tgcaaaacct ggcctggggt ggcgtattta aactgaaaga
    13021 tactggctat ccggtatttc gttttgctaa ggatgtcatc gtcaataata acgaggtgat
    13081 tgaagagcaa gaacggatgg caaaactgtc cggaatgaaa gatacctgga cggtcactgc
    13141 cgttaagccg aagtatcaga cctatgtggt ggtgatcggt gaaagcgcgc gtcgcgatgc
    13201 cctcggtgcc tttggcggtc actgggacaa taccccgttt gccagcagcg ttaacggttt
    13261 gatatttgct gactacattg ccgccagtgg ctccacgcag aaatcgcttg gcttaacgct
    13321 caatcgcgta gtcgatggca aaccacagtt tcaggataac tttgtcaccc tggcaaatcg
    13381 cgcgggcttc cagacctggt ggttttccaa ccagggccaa atcggcgaat acgataccgc
    13441 catcgccagc atcgccaaac gtgcagatga agtgtacttc ctgaaagaag gtaattttga
    13501 agcagataaa aacacgaaag acgaagcgtt actggatatg accgctcaag tgctggcgca
    13561 agagcactcg caaccgcagc tgattgtttt acatctgatg ggctcacatc cgcaggcctg
    13621 cgaccgaaca caaggcaaat acgaaacctt tgtgcaatcg aaagaaacgt cgtgctatct
    13681 ctataccatg acgcaaacag acgatttact gcgcaagctg tacgatcagt tacgcaacag
    13741 cggcagcagc ttctcgctgg tttacttttc tgaccacggt ctggctttta aagagcgcgg
    13801 caaagacgtg caataccttg cccatgatga taaatatcag caaaatttcc aggtgccttt
    13861 tatggtcatt tcgagcgacg ataaagcgca tcgggtgatt aaagcccgcc gctctgccaa
    13921 tgacttctta ggctttttct cccagtggac gggaattaaa gcgaaggaaa ttaacatcaa
    13981 atacccgttt atatctgaga agaaagccgg gccgatatac atcaccaact tccagttaca
    14041 gaaggtggat tacaaccatc tcggaaccga tattttcgac ccgaaacctt aaaaacaaaa
    14101 atccgccccg agaggcggat tttttatatc aacgaagtga ttagaagcgg taaccaacac
    14161 cggcaatcca ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca
    14221 gagcaacgtt ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc
    14281 tggtgtcgtg tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca
    14341 ctacaccgta gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt
    14401 agtactggtt tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga
    14461 aagaaccgat cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac
    14521 cgcccatttt gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag
    14581 aagtcgcagc tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac
    14641 atgcaatttt tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat
    14701 atcaaaaatt aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa
    14761 cagaacgttt ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg
    14821 taagtattac gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat
    14881 gattccatca atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct
    14941 caaccagcat tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg
    15001 gagaaaggat gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc
    15061 tcgttgccat ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg
    15121 tgggcgcacc gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt
    15181 tctttaagcc atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg
    15241 gcgtttcgct gggggggatg aattatcttt tttatctttc tattcagacg gtaccgctgg
    15301 gtattgcggt ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc
    15361 gcccggtaga tttcgtctgg gttgttctgg cggttcttgg tctgtggttc ctgctaccgc
    15421 tggggcaaga cgtttcccat gtcgatttaa ccggctgtgc gctggctctg ggggccgggg
    15481 cttgttgggc tatttacatt ttaagtgggc aacgcgcagg agcagaacat ggccctgcga
    15541 cggtggcaat tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg
    15601 ctggtgaagc actctggcac tggtcggtta ttccattggg tctggcggtc gctattctct
    15661 cgaccgctct gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat
    15721 ttggtacgct gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg
    15781 gagaaacact gacacccata cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg
    15841 ggtctacgct gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt
    15901 acatttctgc atggttatgc ataaccatgc agaatttctc gctacttttc ctctacaccg
    15961 tctttatata tcgaattatg caaaagcata tttattccga aaattcctgg cgagcaaata
    16021 aataagaatt gttcttatca atatatctaa ctcattgaat ctttattagt tttgtttttc
    16081 acgcttgtta ccactattag tgtgatagga acagccagaa tagcggaaca catagccggt
    16141 gctatactta gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt
    16201 accgctaaat tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctct
    16261 gacagcgaga aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat
    16321 ctttctttga ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta
    16381 catgaaatgc tggatggctt ccgcaccgca ctgatcgatc atctggatac catggcagaa
    16441 cgtgcagtgc agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaacc
    16501 ccgctgaaaa gttacccgct ggacatccac aacgttcagg atcacctgaa agaactggct
    16561 gaccgttacg caatcgtcgc taatgacgta cgcaaagcga ttagcgaagc gaaagatgac
    16621 gacaccgcag atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc
    16681 gagtctaaca tcgaataaat ccatcgctga tggtggagaa ctttagtacc cgataaaagc
    16741 ggcttcctga caggaggccg ttttgttttg cagcccacct caacgcactt atttagtgca
    16801 ttcatctgct atctccagct gattaagtaa attttttgta tccacatcat cacacaattg
    16861 ttacataaag attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac
    16921 aattttagcg caccagtttg gtgccccaga atggtgcacc ttcagggtat tgccctataa
    16981 atcgtgcatc acgtttttac cgcatctcga taaatcaatg agttgcaaaa ctggcacgat
    17041 tttttcatat atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc
    17101 tatgaagtct gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc
    17161 tcatgccgcg gataaaaaat tagttgtcgc gacggatacc gccttcgttc cgtttgaatt
    17221 taaacagggc gataaatacg tgggctttga cgttgatctg tgggctgcca tcgctaaaga
    17281 actgaagctg gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca
    17341 aaccaaaaac gtcgatctgg cgctggcggg catcaccatt accgacgagc gtaaaaaagc
    17401 gatcgatttc tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa
    17461 taacgatgtg aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggcac
    17521 tggctccgtt gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa
    17581 cattgataac gcctatatgg aactgggcac caaccgcgca gatgccgttc tgcacgatac
    17641 gccaaatatt ttgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga
    17701 ctctctggaa gcacagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga
    17761 caaagtcaac ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa
    17821 aaaatggttc ggtactgaac cgaaataata acgctacgcc tgtaaggcgc actggcagtt
    17881 ccctctcccc tctggggaga ggattagggt gaggggcgca aacccgctcc ggggccatta
    17941 attaccctga atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg
    18001 gagtgccatc tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat
    18061 ctcggtcctc ggtttggctg gcggtctggt gatcggattg ctggcaggtt ttgcacgcac
    18121 cttcggtggt tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac
    18181 acctatcgtc gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgactt
    18241 acgcatcgac ccgtttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat
    18301 tgcggaaatc acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg aagcaggact
    18361 ggcgcttggt ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg
    18421 tcgtatgctg ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cctcgctgtt
    18481 tattgttatc ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt
    18541 ccgcgccctt gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct
    18601 gagctttatt ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac
    18661 gtctccaagc attttggtcc aacccaggtg ctgcacaata tcgatttgaa cattgctcag
    18721 ggcgaagtcg tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc
    18781 atcaacaaac tggaagaaat cacttccggc gatctgattg ttgatggcct gaaggttaac
    18841 gatccgaaag ttgacgagcg cctgattcgc caggaagcag gtatggtgtt ccagcagttt
    18901 tacctcttcc cgcatctgac ggcgctggaa aacgtcatgt ttggtccgct acgcgtgcgt
    18961 ggcgcgaaca aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg
    19021 gcagaacgtg cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg
    19081 atcgcacgcg cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct
    19141 cttgacccgg aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg
    19201 atgacgatgg tgatcgtgac acacgaaatc ggttttgccg agaaagtagc ttcgcgcctg
    19261 atctttatcg acaaaggccg gattgcggaa gatggcgatc cgcaggtgtt gatcaagaac
    19321 ccaccgagcc agcgcttgca ggaatttttg cagcacgtct cttaacttct cagcctccct
    19381 ttcccccgaa agggaggcac accagattcc tctcatttaa aatcgctcct cccccagcat
    19441 ctatacttat ctttttgctc tgttttctca ctggaggagt catgcggtgg atcctgttca
    19501 tcctcttctg cctgctgggc gcacctgccc acgcggtatc catacccggc gttacaacca
    19561 caacgacaac ggactcaacg actgaaccgg cccctgaacc ggatatcgaa caaaaaaaag
    19621 cggcctatgg cgcactggcg gatgtgctgg ataatgacac ctcgcgtaaa gagttgatcg
    19681 accagttgcg caccgtcgcc gcgacgcccc ctgcggaacc cgtaccgaag atcgtgccgc
    19741 cgacgctggt tgaagagcaa accgtgctgc aaaaagtcac cgaagtcagc cgccattatg
    19801 gtgaagccct ttccgcccgc ttcggacaac tttatcgcaa tatcaccggc tccccgcata
    19861 agccgtttaa tccacaaacc ttcagcaatg cgttgaccca tttttcaatg ttagcggtat
    19921 tagtgtttgg tttttactgg ctgattcgtc tgtgcgcact gccgctgtat cgcaaaatgg
    19981 gccagtgggc gcggcaaaaa aatcgcgaac gcagcaactg gttgcagctt ccggcgatga
    20041 ttatcggggc gtttattatc gacctgctgt tactggcact gacattattt gtcggtcagg
    20101 ttttaagcga caacctgaat gcgggcagtc gcaccatcgc tttccaacaa agtttgtttc
    20161 tcagcgcctt tgccctgatt gaatttttca aagccgtact acgcctgatt ttttgcccaa
    20221 acgtggcgga actgcgcccg ttcacgattc atgacgagac cgcccgttac tggagtcgtc
    20281 gcctgagctg gttaagcagc ctgattggct atggcctgat tgtcgccgtg ccgattatct
    20341 ctaatcaggt gaatgtacag ataggtgcgc tggcgaacgt catcattatg ctgtgcatga
    20401 ccgtctgggc gttgtacctg atctttcgta ataaaaaaga gattacccag catttgctca
    20461 acttcgcgga gcattcgctg gcctttttca gcttgtttat ccgcgccttt gcgctggtgt
    20521 ggcactggct ggcaagcgcc tattttatcg tgctgttttt cttttcgttg ttcgatccgg
    20581 gcaacagcct gaaatttatg atgggtgcaa cggtgcgcag cctggcgatt attggtatcg
    20641 cagcatttgt ttccggtatg ttttcccgct ggctggcgaa aaccatcacc ctctcgccac
    20701 atactcagcg taactatccg gagttgcaaa aacggctgaa tggctggctt tcggcggcgc
    20761 tgaaaacggc gcgtattctg accgtctgcg tggcggtaat gttgctgttg agcgcatggg
    20821 gattgttcga tttctggaac tggctgcaaa acggtgcggg gcagaaaacc gtagatatcc
    20881 tgatccgtat cgcactcatt cttttcttct cggcggttgg ctggacggtg ctcgccagtt
    20941 tgatcgaaaa caggctggct tcggatattc atggccgccc actacccagc gcccgtacgc
    21001 gtaccctgct gacgctgttc cgtaacgcgc tggcggtgat tatcagtacc atcaccatca
    21061 tgattgtgtt gtcggaaatc ggcgttaata tcgcgccatt gctggcaggt gccggtgcat
    21121 tggggctggc tatctctttt ggttcgcaaa cgctggtgaa agatattatc accggggtat
    21181 ttattcagtt tgaaaacggc atgaacactg gagatttggt gactatcggg ccgttgaccg
    21241 gcactgtgga acggatgtcg attcgctccg tgggcgtgcg tcaggatacc ggggcgtatc
    21301 acatcattcc atggtcttcg ataaccacct ttgctaactt cgtccgcggc attggttcgg
    21361 tggtggcaaa ttacgatgtt gatcgccatg aagatgctga taaagccaat caggcactga
    21421 aagatgcggt agcggaattg atggaaaacg aagaaattcg cgggctgatt attggtgaac
    21481 cgaattttgc cgggattgtc ggcttaagca ataccgcatt tacactgcgt gtttcgttca
    21541 ccacgctgcc actcaaacag tggacggtcc gctttgccct cgacagccag gtgaaaaaac
    21601 atttcgacct ggcgggtgtt cgcgcgccgg tacagactta tcaggtgctg cctgctccgg
    21661 gcgcggcccc ggctgaaccg ctgccgccgg gggaaccaac gctttaacgc tggcgattga
    21721 caaaacggcg gcgctgctcg tcgttcataa aggtccaggc aataaagcgg ctctgctttt
    21781 gcccctgggc catctctttt ttgaccactt tcaccgcgcc cacgtccgtc agagcacgat
    21841 acaacggcgg caagttttcg ccacgagata ccagcgaggt aaaccacatc acctgcttcg
    21901 cgaagccttt gctctcttca atcatctttt tgataaaagc gacttcaccg ccttcacacc
    21961 acaactcctg ttgctgacca ccaaagttca gtgcatcgtc tttgttcagc cccaggttac
    22021 ggcgtttacg ttcactacct gcccgtgccg cagcggcgga atcgtggaat ggcgggttac
    22081 acaaggtcgc gtcgtattgc tcgtttttat ggatgatgcc gttaaaaatc gccccactct
    22141 ctttttgccg acgcagacga atggcgcggt taagccccgg attagcactg atgatcgcct
    22201 gcgcactgct taacgcctgg ctgctggttt cactaccagt aaaacgccag ccatattcat
    22261 gtacaccaat cagcgggtaa atacagttcg caccaacgcc gatatccaga atgctggcat
    22321 tagctggaat tgttccgctt gcttctgcca gtaaatcggc aaggtgatga atataatccg
    22381 cccggcccgg tactggcggg cagagaaaac cgtcggggat atcccagttc gctacggcgt
    22441 aaaaatgggc cagcaacgcc ttattgagcg ccttcaccgc cagcggattg gcaaagtcta
    22501 cgctttgctc cccggcgggt gtaagcgtga ggaattgcct gagttcagga ttgacctgac
    22561 aaagcgtggc gagatcgtag cggctgtgat gacggttgcg cggatgcaac cccggtttct
    22621 gggcggacat agcattctcc tttaagcagc ggcgtaagat acccgcataa cgcgacaggg
    22681 taaatatctg gctgcgttta tcatttctaa aaaccttaac atggctatgg tttatggcaa
    22741 ttcatcataa aaatcagcat catgcatgtc attgatttgt cttattttgt aaaaatatat
    22801 cgttcaaaaa ataagcgtat ctctgtcaat tttttgcgtc attttcatcg cccaggcgac
    22861 ctacacttat caggcactac ccgttgtatt tatgaggatg tcattatgaa aaagtgcctc
    22921 acactactga ttgccaccgt cctgagcgga atctctctca cggcttatgc cgcgcaaccg
    22981 atgagtaacc tggacagcgg tcagctacgg cccgccggta ccgtttcggc aactggcgca
    23041 tcaaacctaa gcgatctgga ggataaactg gcagaaaaag cgcgcgagca aggcgcgaaa
    23101 ggttatgtca ttaattccgc aggcggaaat gaccagatgt tcggtactgc aaccatctac
    23161 aaataacccc accaacgtaa ctgcattcgc ctctggatgc agttttcgcc tgttttgtaa
    23221 tcaaaaaaca tcaaatatga acgtaatgta aataaatgta tttctttttc gcacaatggg
    23281 tgatagaaaa tcgcgccaag tgataatgct tatcaaaatt attatcactt gcacgagcgc
    23341 tatcacgaga ttaacagtgg catcgcatcc gcagagaggc tttctcgtgg cagtgaaaat
    23401 ttcaacatat aagaaaaagt cacctgcaaa atggaaaaca atcgcaattt ccctgccaga
    23461 caatttcatt cgctcacgtt ctttgccggt ctttgtattg gcatcacgcc tgtggctcag
    23521 gcactcgccg ccgaagggca aactaacgcg gatgacacgc tggttgtcga agcatcaacg
    23581 ccttcgcttt atgcgccaca acaatctgca gatccgaaat tctcgcgtcc ggtagcggat
    23641 actacccgca cgatgacggt gatttctgaa caagtgatta aagatcaggg cgcaaccaac
    23701 cttaccgacg cactcaaaaa cgtccccggc gtgggtgcgt tttttgcggg tgagaacggt
    23761 aactccacca ctggcgacgc catttatatg cgcggtgctg atacctctaa cagtatttat
    23821 attgatggca ttcgcgatat cggcagcgtc tcgcgcgaca ccttcaatac cgagcaggtc
    23881 gaagtgatta aagggccgtc cggcaccgac tacgggcgca gcgcaccgac aggctcgatc
    23941 aatatgatca gcaagcagcc gcgcaatgat tccggcattg acgcctccgc cagtattggt
    24001 agcgcctggt tccgccgcgg cacactggac gtcaatcagg tcattggtga taccaccgcg
    24061 gcgcgcctga atgtgatggg cgaaaaaacg cacgatgccg gacgcgacaa agtcaaaaat
    24121 gagcgttacg gcatcgcccc ttctgtcgct tttggccttg gtacagcgaa tcgtttgtat
    24181 cttaattatc tgcatgtcac ccagcacaac acgccagacg gcggcattcc gaccatcggt
    24241 ttgccgggct attctgcccc atctgcggga acggcggccc tgaatcattc cggaaaagtt
    24301 gatacgcata acttttacgg cacggattcc gattacgacg attcgaccac cgacaccgct
    24361 accatgcgtt ttgagcacga catcaacgat aacaccacca ttcgcaatac tacccgttgg
    24421 tcgcgcgtga agcaggatta cctgatgacg gcgattatgg gtggggcgtc gaatattacc
    24481 cagcccacca gcgatgtgaa tagctggacc tggtcacgca cggcgaatgc taaagatgtg
    24541 agtaataaaa ttctcaccaa ccagaccaac ctgacctcga cgttctatac cgcttctatc
    24601 ggtcatgatg tcagtaccgg cgtggaattt acccgtgaaa cgcagactaa ctacggcgtt
    24661 aatccggtga cgttacctgc ggtaaatatt tatcatcctg acagcagcat tcatcccggc
    24721 ggcctgacgc gcaacggcgc aaacgccaat ggtcagacgg ataccttcgc aatttacgcc
    24781 ttcgatacgc tgcaaatcac ccgtgatttt gagctgaacg gcgggatccg tctggataat
    24841 tatcatactg aatatgacag tgccaccgcc tgcggcggca gcggacgcgg tgccatcacc
    24901 tgccctgctg gcatggcaaa aggttctccg gtcaccaccg tcgataccgc taagtcgggc
    24961 aatctggtga actggaaagc cggggcgctt tatcacctga cggaaaacgg caatgtctat
    25021 attaactatg ccgtttccca gcagcctccg ggcggcaaca acttcgccct tgcgcagtct
    25081 ggcagcggta acagtgccaa ccgcaccgat tttaaaccgc aaaaagccaa caccagcgag
    25141 attggcacca aatggcaggt tctggataaa cgcctgttgc tcaccgccgc gctgttccgt
    25201 actgatatcg aaaatgaagt tgagcaaaat gatgacggaa cttactcgca atacggtaag
    25261 aaacgcgtcg aaggctatga gatatccgtg gccgggaata tcactcccgc gtggcaggtg
    25321 attggcggct atacccagca aaaagcaacc atcaaaaacg gcaaagatgt tgcccaggat
    25381 ggttcctcat cgctgccgta taccccggag cacgccttta ccttatggag ccagtatcag
    25441 gcaaccgatg atatttccgt tggtgcgggc gcacgctata tcggcagtat gcataaaggt
    25501 tctgacggcg cggtgggaac gccagcgttt accgaaggtt attgggtcgc cgacgctaaa
    25561 ctggggtatc gggttaatcg caatctcgac ttccagctaa acgtctacaa cctgtttgat
    25621 accgattacg tcgcctcaat caacaagagc ggctaccgtt atcacccggg cgagccaaga
    25681 accttcctgc tcacggccaa tatgcatttc tgattcagat gtggggcgca ggccccactt
    25741 tttggagaac ttgtatgatg taccacattc ccggcgtgtt atcgccacag gacgtcgccc
    25801 ggtttcgcga acaactggaa caagccgaat gggttgatgg acgcgtcacc accggcgcac
    25861 aaggtgcgca agttaagaac aatcaacagg tagacacccg cagcacgtta tacgccgccc
    25921 tgcaaaatga ggtgctgaac gcggttaacc aacatgcttt gttctttgcc gcggccttgc
    25981 cgcgtaccct ttccacgccg ctgtttaatc gctatcagaa caatgaaacc tatggttttc
    26041 atgtggatgg cgcagtacgc agccatctac aaaacggctg gatgcgcact gacctttctg
    26101 ccacgctgtt tttaagcgat ccagaaagct acgacggcgg cgaactggtc gttaatgaca
    26161 ccttcgggca acatcggata aaactcccgg caggcgatct ggtgttgtat ccctccagca
    26221 gcttgcattg cgtgacaccc gtaacccgcg gcgtacgagt ggcatcattt atgtggatcc
    26281 agtcgatgat ccgtgatgat aaaaagcgcg ccatgctgtt tgaactggac aacaatattc
    26341 agtcgctgaa aagccgctac ggcgaaagtg aagagatcct gtcgctgctc aatctttatc
    26401 ataatctgct gcgggaatgg tcggagatct gacgcttgaa ctgcccgagt tcgtccctat
    26461 attagctaca cttaactgta caagtattga tatggggagg acgatatggc atccggttgg
    26521 gctaacgatg acgccgtcaa cgaacagatc aacagtacaa ttgaagatgc gattgcccgc
    26581 gctcggggtg aaattccgcg tggtgaaagc ctgtatgaat gtgaagagtg cggcgcacct
    26641 atcccacagg cccgtcggga agccattcct ggcgtgcgct tatgcattca ttgtcagcag
    26701 gagaaagatt tacaaaaacc agcttataca ggatataatc gcagaggttc gaaagacagc
    26761 cagttacgtt aactacaact ggcgggatgt atagcgagta taatttgtat tttgcgtggc
    26821 attccgtgcc tttaacgctt tcgtgactgc cacgctgcat aaaacaagtc cataagttca
    26881 acaaataaat ggattatcag tatgttagat atcaatcaat ttatttgaac aaggtggtca
    26941 attctcttcg attttatctc tcgaaaaaaa acgtgatact catcacatcg acgaaacaac
    27001 gtcacttata caaaaatcac ctgcgagaga ttaattatga aaactatcaa tactgttgtt
    27061 gctgctatgg ctctttcaac tctgtcattt ggcgtgtttg ccgcggaacc ggtaacggca
    27121 tcccaggcac agaacatgaa taaaatcggc gtggtttctg ccgatggcgc atccaccctc
    27181 gatgccctgg aagcgaaact ggctgagaaa gctgcggcag ctggggcgag tggatacagc
    27241 atcacttctg ccaccaacaa caataaatta agcggtactg cggtaattta taagtaattc
    27301 gtaggcggat aaggcgtttc cgctacgtcc agcacaagta tttccctgtc tgtttgccga
    27361 cagacgcata tgctctaacc ctcattgatc ctatgttacc cttgtttgcc cgtccgccac
    27421 tggacgggct tttttttagc tggttaactg ctggcagaaa gcctgcaacg tctcttccgg
    27481 cataccaatc tgccgcgccg catcacaaat ggcctgccag cttcccgcat ccagtggaat
    27541 cccctgctcc tgccgttcgc gacgcgtgtt aacttcccac tcgcccggta gcaaaatcga
    27601 cttatcgtca tcatgcggcg aggctttcac ccactcggca aaggcttcgg tctgcgcgct
    27661 acaatccggc gcgccgaaca gttccgggtt gatgatgata gtggtcatgc agttaagaat
    27721 ggcatcggga ctggtttgta acgtttcctg atgcgtcgtt ttaccgccgg aaagcgcccc
    27781 accaagaatt tcacacatcg ctgcaagggc gtagccttta tgttcggcaa aggtcaacag
    27841 cgaacccaac ggcgactcct gcattaccgc cggattggtc gtcggcacgc cgttaacgtc
    27901 aatcaggcaa cctggcggca cggggacgcc tttatgccag gcgacgcggg ttttgccaaa
    27961 tgcaatggcg ctggtggcgt aatcaagcaa cagcgggaac ttatctttac gagggaaaac
    28021 cacacagaag ggattggtgc caaagcggct gtcgcgaccg tggaacggcg cgaccattgg
    28081 aatgccgacc acgctaacaa agtggataga gacaaacccc gccgctgcgc actgttctgc
    28141 ccagtaaccg atacggccga tatgatgcga gttatgtagc gccacggcag caataccgtg
    28201 ctgatgcgct ttctcaatcc ccagtgccat cgcttcgtgt gctgcgacct gaccaaatgc
    28261 gcgatcgcca tcgagcgtga ccgccgcccc cgcctcttta acgattttgg catgatggtt
    28321 aatttgcaga tgcccctgac tccaggagcg cacatagctt gggatcatgc caataccatg
    28381 tgaatcatgc cctgccaggt ttgccgcgat taaatgatcg gcaactaatt tcgcttcttg
    28441 ttcctcgcta cccatctgac gaaatacagc ctgaataaaa ctgtgcaacg tctgagcatc
    28501 aaagcgatga ccactttcca tggttaactc ctgtttatgt tatgtgtttg ttgttttttt
    28561 atgctgcacg ccgggcgcgg gaaggtcaat aaagatgcag gaatttcata ttgcggtggg
    28621 gtaaaagccg gatgacgtgg ctcatccggc tgagataaaa attaaaatgc ctgattaacg
    28681 cgcgccaggc catcgctgat agttgccgct tcccccgtag ccaccaggca gcaagccatc
    28741 tggattttca gcgattcggg aatcgcttcg ctgccagcca ggcaacgctc aatccactgc
    28801 gccgtggttt ccggatcttt tgcttgcggc agtaactcgc taccagcagg gtcctgtttt
    28861 tcatacagca cccgcatccc ttcatggtcg atcaaattga tctgcgggca gcgttgcgga
    28921 ttagcataca cttcaccttc agtaccatgc attagtagcg cccgcccgcc gatatcgcta
    28981 aagaacttcg cgacgcgtcc aatgtattcc ggatgcgaaa cgctggaaag acgcagtgcc
    29041 tcaccttcgg caaatggcgt cgccagtttc gccagggtat gtgcactgtt acgcacgccc
    29101 atccgccagc gcatcgccag ttgtttttcc agcggcgggc aaaacgcgcc gacaggcata
    29161 aagacaggtt gatgttcgtc gagcttcgcc tgcgcctgcc cgccgtgcaa cgttggcgta
    29221 atacccatca gttcgaaaat ggtttctgtc agcacgcggg ttggatcttc gctaaccccg
    29281 tgaaccacca caggaaaacc gagtttgtgc aggagaatcg ccaacaacgg cgtcaggttg
    29341 gcctgcttac gcgcgccgtt gtaactgggg atgacaatcg gcatcggctt gtctgcaggc
    29401 ggcgtcagct tgatggtgtg attttgcatg gcttcgtaaa agccgagcat ctctgcttcc
    29461 ccttcccctt tgatacgcag cgcaatcaat acgccgccca actcaaggtc agggacttca
    29521 ccattgagca tatgggcata cagaccgcgc gcagtatccc ggtctaaatc gcgcgcgtgg
    29581 tttttcccgc gcccgatctc tttaatgatt ttgcgatagt ccatttacga ctccttacct
    29641 gactcacatc attaacgccg ccgacggcgt ggggatttcg ttttttcttt ctttttaact
    29701 ataccttcag ggacttccgg ttgctctatc ggaaataccg gtaatgcatc cagtagtcgc
    29761 ttgccataat ttttggtcag caagcgtttg tcgtagataa ccacttcccc ccagcaaccg
    29821 tggcttcgaa tcagtcgccc aacctgctga atcaggttaa acgaagcgct cggcaggctt
    29881 tgcacctcaa acggatagcg gttgaggctt ttcagccatt ccccttcggt gatcaccacc
    29941 gggctgtcga tgggcggaaa agcgattttg tggatatgca cctggctgag cagatcgcct
    30001 ttcagatcaa gaccttcggc aaaggactgt aagcccacca gcacgctacg ctcaccgttg
    30061 gcaacgcgtt tgcggtgcag ttcaactaag cgataacgtg gctgatcgcc ctgcaccagc
    30121 aacatcaggc gtaaatccgt cacatagtca agaaagcgct gcatcgcccg tccgctggca
    30181 aacagtacca acataccgag atgttttttg ctctccacct gctcacggaa aaaggccgcc
    30241 atttcggcaa tatgctgctc ttcgttgtcg atggaaggct caaagcgcat ccggggaata
    30301 acaattttgc cctgttcgca gtggttaaag ggggaatcca gcgccacaaa acggtcgccc
    30361 gctttctctt tcagcccact catctcctgc aaacgcgaaa aactattcag cgaacgcaag
    30421 gttgcggagg tgacaataat gtgcggaata ctgcgccaca gcagcctttc cagctgatcg
    30481 ctgacacgaa tacccacaca gtgaaaccag agatgcagct gcccttcgcg ctcttcccgt
    30541 gtcgcccatt tagtcaccgg cgcaccggaa gattgcgcca gagacgccag ccgccagagt
    30601 ttgctttgcg cctcgaacat ccccagcgcg cgattcatct gcaaaatcaa ccgatgcaga
    30661 cgtacaatgt catggctgcc ggttttctca cttaaatcgt taagaaataa ctcagccagg
    30721 ccacgcagca tctcggtgag ttttgccagc cgctggcaga tctccagcac ttcatctggc
    30781 agttcgccca tcgcaaaacg gtgctctgcc tcttgcccgg caggcatgta gagattgaga
    30841 atgttgttta acgtggcgat aagctcatac aactcttcac aatgcgcatt caaacgttca
    30901 gggatcgcca gcggcgggat ggtcttcggg cgaaactgct ccatgcaggt agcgaccagt
    30961 ttcgtgaaca agtccagctg tagtcgatac cagggcgcgg tgatttcggc actcatctcc
    31021 agcgcatccc gcgccacatc cggcaggtga tggccttcgt ccagcaccag cagtaaattt
    31081 ttcgggtcag gcaatacggc ttcgctttcc atcgccgcca tcaccagcgc atggtttgcc
    31141 accaccactt ccgcttcctg aatctcccga cgagcaacaa aaaacgggca ttcacgatag
    31201 tagtaacagt tgcggttgag gcagctggct ttgtcggtac ttaaacgacg ccagagatcg
    31261 tcatctatag cgatatcagt atgatcgcgc agaccatccc atttataagt gtcgagatcg
    31321 cccttcagct tcgcacaacg tttttgctct tcctgattgt tcggcgtcag ctcgtcgtca
    31381 agaaacgcca gcagatcctg ttgcgtgggc tcagtgctgg caagcgccgt cagattacgc
    31441 gggcaaacgt agcgcccacg cccaaaagcg gcagtgaatt taagatcggg aatgatcttt
    31501 ttcagcagcg gtaaatcttt actgtagatc tgatcctgca atgccacgtt ggcggtactc
    31561 accaccagcg ttttttgctc ttcgcgggca atggcgatgc cggggatcaa ataggagagc
    31621 gttttcccaa cgccggtggg ggcttcaatc gccagatgcc gcccttcttc tccggccagc
    31681 gttttggcga cgtccgcaat catctgccgc tgcggcgcac ggggaataaa gtcggggatc
    31741 tgttcctgaa gcgccttata ccaggcggca atttgcgctt taagcgcggc ggttaatgcc
    31801 atgagaaaac ctgaaatact gtataaacag ccaatattgt ggcatttttc ggttttcagg
    31861 ggtaatggtt tttctgtttc tggaaagcgg ctcgcaataa cgttttccgg atgtgaagtt
    31921 ttctcgcggc agaagaaaca tcattgtgca gcaaaagaca ttgtacatca gcttattccg
    31981 ctggcggcca ttgcagagcg gtatgtaaaa cacgcagtac cgagagcgta tcgccctcca
    32041 cttgatagac aagcacataa tgcggcctga caacggcttc atgtgtacca gcgactcgtc
    32101 cggcccggta catctccggg cgttcacagg cctgttccgc aatggcttca aattgattat
    32161 caagggctat tgccgctagc ggattatctt tactgatgta ttccatgatt tgctccctgt
    32221 cagacaggga cattggcttc cattccagac gcattaatcc cccttcagca aggctttttt
    32281 acgggcagca aatagacttt tggcctcttc atgagagagt gccgggcgtg gatcgtcaat
    32341 ggcggcctgc acctgctctc gaaaccaggc atcatacgcg gcagcttcat gagctcgttt
    32401 taatgcctca gccctgtctg gtcgggtcgt tttcttctga ccgtccgggt cgtaattaga
    32461 ggcgtcgact tcaaaccggg caataccaac atctttcagg tagttgacaa ccgtttccag
    32521 ccgtttgaac aggcggattt gccgactgcg ttgtgctgca agaggccgtt cagtgtggcc
    32581 gtatttcacg gtcaacgacc agcctccctg ggtgccaata acgtgtgctg catccactac
    32641 gccagattca accagtttat gtaaggttga gtgatcgatc gtttctggtg ccataatgcg
    32701 ctaacctctt tgtatacagc atgcaattta taattaaaga tagagcaatt aataataaat
    32761 tgcagcacct tgatcgtaaa ttgcccagga taaaattgcg gtagaaagag acttgttgcg
    32821 aagatgccgg gcatcgcccg gcataaaaga ttactgcgca gcggcaggtt tacgcggacg
    32881 gcggcggcgt tgttgttcgc ctgccggttt ggcatcgcca agacgacgag atggtttttc
    32941 tgcaggcttc gcgctcgcag attttgcgcc accttcagcg cggcgtggtt gctgttgacc
    33001 gcgaccacca ccttgcccac gaccgccgcc gccacgttgc tggcgaccgt tctggattgg
    33061 ttcggctttg attgacgggt ccggctcata gcccggaatc gcaatgcgcg ggatctcttt
    33121 tttcagcagt ttttcgatat cacgcagcag tttgtgttca tcaacacaca ccagcgacaa
    33181 cgcttcaccg gtagcagccg cacgaccggt acgcccgata cggtgaacat aatcttccgg
    33241 tacgtttggc agttcatagt tgaccacgtg cggcaactct tcgatatcca gaccgcgcgc
    33301 agcgatgtca gttgccacta gtacacgaat atcgcccgat ttaaaatcag ccaacgcacg
    33361 ggtacgcgca ccttgcgatt tattgccgtg gatcgccgca ctgcggatgc catctttatt
    33421 gagctgttca gccagatggt tagcgccatg tttggtacgg gtaaacacca gcacctgctg
    33481 ccagttccct ttcccaatca tgtgcgacaa caattcgcgt ttgcgtttct tatcaacaaa
    33541 gtgaacatgc tgggtcacct gatcagacgc ggtattgcgg cgtgccactt cgatttccag
    33601 cgggttgtgc aacagttttt ccgccagagc tttaatatcg tcagagaagg tcgcggagaa
    33661 caacaggttc tggcgcttcg caggtagttt tgttaacacg cgacggatat cgtggataaa
    33721 gcccatgtcg agcatgcggt cagcttcatc gaggacgagg atttcaacct gatccagctt
    33781 caccgcattc tgatgttcca ggtccagcag acgtcccggc gttgcaacca gcacatcaac
    33841 gccgccgcgc aacttcatca tctgcgggtt aatgctgaca ccgccaaaca ccaccagcga
    33901 acgaatgttc aggtatttgc tgtaatcacg gacgttttcg ccaatctgcg ccgccagttc
    33961 acgggtcggg gtaagaatga gcgcacgtac cggacgacgc cctttggcgt gcggctggcg
    34021 agtgatcagg tgttgcaaca gcggcagcgt aaagcccgct gttttgccgg tgccagtctg
    34081 ggcgctagcc atcaggtcgc ggccttccag caccgcaggg atcgcctgct gctgaatagg
    34141 ggtgggttca cggtaaccct gctcggcaac ggcgcgcagg atatcagggc ttaaacccaa
    34201 agaatcgaaa gacataacta ctccgaaccg ccccgaccgt taccggtgta gttttcaggg
    34261 agatactgaa aaggaaaatg acaaaaacca caatgtcatg agggagcgga gtgtagcagt
    34321 ttttgtgacg cagcgcataa attatccctg tgactggcgt tgctaaaatt cctggtcata
    34381 ctggacaact cccatatgga gtcataatct taatcaatca tttgattaag aatggactgt
    34441 gcgatgaata atcctgccat gacaatcaag ggtgaacagg cgaaaaaaca gctgattgct
    34501 gccgcactgg cgcagtttgg tgaatatgga atgaacgcca ccacccgcga gatagccgcc
    34561 caggccgggc agaatatcgc tgccatcacc tactacttcg gttcgaaaga agatttgtat
    34621 ctcgcctgcg cccagtggat tgccgatttt attggcgagc agttccgtcc gcacgccgaa
    34681 gaggccgaac gcttgttcgc acaaccacag ccagatcggg ctgccatcag tgaactgatc
    34741 cttcgcgcct gcaggaacat gattaagctg ctcacccagg atgacactgt caacctcagc
    34801 aaatttatct cccgtgagca gctctctccc acggcagcct atcacctggt gcacgaacag
    34861 gtgattagtc cgctacacag ccaccttaca cgcctgattg ccgcctggac cggctgcgat
    34921 gccaatgaca cccgcatgat ccttcatacc catgcgttga ttggtgagat tctggcgttt
    34981 cgtcttggca aagaaacaat tctgttacgt accggctgga ccgcgttcga tgaagaaaag
    35041 accgaactga tcaaccagac ggtgacctgt catatcgacc tgattttgca aggattatcg
    35101 caaaggagtt tgtagtgatg aaaaaacctg tcgtgatcgg actggcggta gtagtacttg
    35161 ccgccgtggt tgccggaggc tactggtggt atcaaagccg ccaggataac ggcctgacgc
    35221 tgtatggcaa cgtggatatt cgtacggtga atcttagttt ccgtgttggg gggtgcgttg
    35281 agtcgctggc agtggacgaa ggtgatgcga tcaaagcggg ccaggtgctg ggcgaactgg
    35341 atcacaagcc gtatgagatt gccctgatgc aggcgaaagc gggcgtttcg gtggctcagg
    35401 cgcagtatga cctgatgctt gccgggtatc gcgatgaaga aatcgctcag gccgccgcag
    35461 cggtgaaaca ggcgcaagct gcctatgact atgcgcagaa cttctacaac cgccagcaag
    35521 gactgtggaa aagccgcacc atttcggcaa atgacctgga aaatgcccgc tcctcgcgcg
    35581 accaggcgca ggcaacgctg aaatcggcac aggataaatt gcgtcagtac cgttccggta
    35641 accgcgaaca ggatatcgcc caggcaaaag ccagcctcga acaggcgcaa gcgcaactgg
    35701 cgcaggcgga gctgaattta caggactcaa cgttgatagc cccgtctgat ggcacgctgt
    35761 taacgcgcgc ggtggagcca ggcacggttc tcaatgaagg tggcacggtg tttaccgtct
    35821 cgctaacacg tccagtgtgg gtgcgtgcct atgttgacga acgtaatctt gaccaggccc
    35881 agccggggcg caaagtactg ctttataccg atggtcgccc ggacaagccg tatcacgggc
    35941 agattggttt cgtttcaccg actgctgaat ttaccccgaa aaccgtcgaa acgccggatc
    36001 tgcgtaccga cctcgtctat cgcctgcgta ttgtggtgac cgacgccgat gatgcgttac
    36061 gccagggaat gccagtgacg gtacaattcg gtgacgaggc aggacatgaa tgatgccgtt
    36121 atcacgctga acggcctgga aaaacgcttt ccgggcatgg acaagcccgc cgtcgcgccg
    36181 ctcgattgca ccattcatgc cggttatgtg acggggctgg tagggccaga cggtgcgggt
    36241 aaaaccacgc tcatgcggat gctggcggga ttactgaaac ccgacagcgg cagtgccacg
    36301 gtgattggct ttgatccgat caaaaacgac ggcgcgctgc acgccgtgct cggctatatg
    36361 ccgcagaaat tcggcctgta tgaagatctg acggtgatgg agaacctcaa tctgtacgcg
    36421 gatttgcgca gcgtcaccgg tgaagcacgg aagcagactt ttgctcgcct gctggagttt
    36481 acatctcttg gcccgtttac cggacgcctg gcgggcaagc tctccggtgg gatgaaacaa
    36541 aaactcggcc tggcctgtac cctggtgggc gaaccgaaag tgttgctgct cgatgaaccc
    36601 ggcgtcggcg ttgaccctat ctcacggcgc gaactgtggc agatggtgca tgagctggca
    36661 ggcgaaggga tgttaatcct ctggagtacc tcgtatctcg acgaagccga gcagtgccgt
    36721 gacgtgttac taatgaacga aggcgagtta ctgtatcagg gggaaccaaa agccctgaca
    36781 caaaccatgg ccggacgcag ctttctgatg accagtccac acgagggcaa ccgcaaactg
    36841 ttgcaacgcg ccttgaaact gccacaggtc agcgacggca tgattcaggg gaaatcggta
    36901 cgtctgatcc tcaaaaaaga ggccacacca gacgatattc gccatgcgaa cgggatgccg
    36961 gaaatcaaca tcaacgaaac tacgccgcgt tttgaagatg cgtttattga tttgctgggc
    37021 ggtgccggaa cctcagaatc gccgctgggt gcaatattgc atacggtgga aggtacacct
    37081 ggcgagacgg tgatcgaagc gaaagaactg accaagaaat ttggtgattt tgccgccacc
    37141 gatcacgtca actttgccgt caaacgcggg gagatttttg gtttgctggg gccaaacggc
    37201 gcgggcaaat cgaccacctt taagatgatg tgcggtttgc tggtgccgac ttccggccag
    37261 gcgctggtgc tggggatgga tctgaaagag agttctggga aagcgcgcca gcatctcggc
    37321 tatatggcgc aaaaattttc gctctacggc aacctgacgg tcgaacagaa tttacgcttt
    37381 ttctctggtg tgtatggctt acgcggtcgg gcgcagaacg aaaaaatctc ccgtatgagc
    37441 gaggcatttg gcctgaaaag tattgcctca cacgccaccg atgaactgcc attaggtttt
    37501 aaacagcggc tggcgctggc ctgttcgctg atgcatgaac cggacattct gtttctcgac
    37561 gaaccgacgt ccggcgttga tcccctcacc cgccgcgaat tctggctgca catcaatagc
    37621 atggtagaga aaggcgtcac ggtgatggtc accacccact ttatggatga agcggaatat
    37681 tgcgaccgca tcggcctggt gtaccgtggg aaattaatcg ccagcggcac accagacgat
    37741 ttgaaagcgc agtcggccaa cgatgagcaa cccgatccca ccatggagca agcctttatt
    37801 cagttgatcc acgactggga taaggagcat ggcaatgagt aatccgatcc tgtcctggcg
    37861 tcgcgtgcgg gcgctgtgcg tcaaagagac acggcagatc gttcgcgatc cgagtagctg
    37921 gctgattgcg gtagtgatcc cactgctact gctgtttatt tttggttacg gcattaacct
    37981 cgactccagc aagctgcggg tcgggatatt gctggaacag cgtagcgaag cggcgctgga
    38041 tttcacccac actatgaccg gttcgcccta catcgacgcc accatcagcg ataaccgtca
    38101 ggaactgatc gccaaaatgc aggcggggaa aattcgcggt ctggtggtta ttccggtgga
    38161 tttcgccgaa caaatggagc gcgccaacgc caccgcaccg attcaggtga tcaccgacgg
    38221 cagtgagccg aataccgcta actttgtgca ggggtatgtc gaagggatct ggcagatctg
    38281 gcaaatgcag cgagcggagg acaacgggca gacttttgaa ccgcttattg atgtacaaac
    38341 ccgctactgg tttaacccgg cggcgattag ccagcacttt attatccccg gtgcggtgac
    38401 catcatcatg acggtcatcg gcgcgattct cacctcgctg gtggtagcgc gagaatggga
    38461 acgcggcacc atggaggctc tgctctctac agagattacc cgcacggaac tgctgctgtg
    38521 taagctgatc ccttattact ttctcgggat gctggcgatg ctgctgtgta tgctggtgtc
    38581 agtgtttatt ctcggcgtgc cgtatcgtgg gtcactgctg attctgtttt ttatctccag
    38641 cctgttttta ctcagtaccc tggggatggg gctactgatt tccacgatta cccgcaacca
    38701 gttcaatgcc gctcaggtcg ctctgaacgc ggcttttctg ccgtcgatta tgctttccgg
    38761 ctttattttt cagatcgaca gtatgcccga ggtgatccgc gcggtgacgt acattattcc
    38821 cgcacgttat ttcgtcagca ccctgcaaag cctgttcctc gccgggaata ttccggtggt
    38881 actggtggtg aacgtgctgt ttttgatcgc ttcggcggtg atgtttatcg gcctgacgtg
    38941 gctgaaaacc aaacgtcggc tggattaggg agaagagcat gtttcatcgc ttatggacgt
    39001 taatccgcaa agagttgcag tcgctgctgc gcgaaccgca aacccgcgca attctgattt
    39061 tacccgtgct gattcaggtg atcctgttcc cgttcgccgc cacgctggaa gtgaccaacg
    39121 ccaccatcgc catctacgat gaagataacg gcgagcactc ggtagaactt actcaacgtt
    39181 ttgcccgcgc cagcgccttt acccatgtgc tgctgctgaa aagcccgcag gagatccgcc
    39241 cgaccatcga cacacaaaag gcgttactac ttgtgcgttt cccggctgac ttctcgcgca
    39301 aactggatac cttccagacc gcgccgttgc agttgatcct cgacgggcgt aactccaaca
    39361 gtgcgcaaat tgccgccaac tacctgcaac agatcgtcaa aaattatcag caggagctgc
    39421 tggaaggaaa accgaaaccc aacaacagcg agctggtggt acgcaactgg tataacccga
    39481 atctcgacta caaatggttt gtggtgccgt cgctgatcgc catgatcacc actattggcg
    39541 tgatgatcgt cacttcactt tctgttgccc gcgaacgtga acaaggtacg ctcgatcagt
    39601 tactggtttc gccgctcacc acctggcaga tattcatcgg caaagccgta ccggcattaa
    39661 ttgtcgccac cttccaggcc accattgtgc tggcgattgg tatctgggcg tatcaaatcc
    39721 ccttcgccgg atcgctggcg ctgttctact ttacgatggt gatttacggt ttatcgctgg
    39781 tgggattcgg tctgttgatt tcatcactct gttcaacaca acaacaggcg tttatcggcg
    39841 tgtttgtctt tatgatgccc gccattctcc tttccggtta cgtttctccg gtggaaaaca
    39901 tgccagtatg gctgcaaaac ctgacgtgga ttaaccctat tcgccacttt acggacatta
    39961 ccaagcagat ttatttgaag gatgcgagtc tggatattgt gtggaatagt ttgtggccgc
    40021 tactggtgat aacggccaca acagggtcag cggcgtacgc gatgtttaga cgtaaggtga
    40081 tgtaacttct tatctttcgc cagcaaagac accaccgccg ggccggcaag gattgccagc
    40141 cctgcaatcg ccagcaaaaa gttgttttcc agtacgcggg acaacagcca cagcacaatc
    40201 agcgctgcgc caaaatacca ggtcgtagtc agctcttcca gcacgtctgc cacctcacgc
    40261 cagcgttgct cggggtggcg ctgaaacacc agcatcatca ctaccgtgac acaatacagt
    40321 gcgcataatc ccacgccgac gtgaaccaga gtcttactca tccagcccac caccagcagc
    40381 gccactacca gtaaatgcaa cataatctgc cagcaactta gacccgttgc gacacgaaca
    40441 cgttgttgcc acttcatggc ttctctcctg aagaatttat ctctacttac tcagagtacc
    40501 gaactttacg gaaaattccg taagaccgca cctttttgtg acgccgacta cactatttca
    40561 atgagataag aggcaaagac aggaaaatta tgcccgatca aacacaacaa ttttcgttca
    40621 aggtgctcac catcaacatt cacaaaggct ttaccgcgtt taaccgacgc ttcattttgc
    40681 cggaacttcg cgacgccgtg cgtaccgtca gcgccgatat tgtttgcctg caggaagtga
    40741 tgggcgcgca cgaagttcat ccgctgcatg tggaaaactg gcccgatacc tcgcactacg
    40801 agtttctcgc cgacactatg tggagcgatt ttgcctacgg tcgcaatgcc gtatacccgg
    40861 aagggcatca cggtaacgcc gtactgtcgc gctatcccat tgaacattat gaaaaccgcg
    40921 atgtttcggt cgatggtgcg gaaaagcgcg gcgtgctcta ctgccgcatt gtgccgccga
    40981 tgaccggaaa agcgattcat gtaatgtgcg tacatttggg cctgcgtgag gcgcaccgtc
    41041 aggcgcagct tgcgatgctc gccgaatggg tgaatgagct accggacggc gaaccggtat
    41101 tggtggcggg tgatttcaat gactggcggc aaaaagctaa tcatccgtta aaagtgcagg
    41161 ccggactgga tgagattttt acccgcgccc acggacgccc ggcgcgcacg tttccggtgc
    41221 aatttcctct actacgactg gacaggatct acgtcaaaaa tgccagcgcc agcgcgccaa
    41281 ccgcgttgcc gctgcggaca tggcgacacc tttctgatca tgccccttta agtgcggaga
    41341 ttcatttatg aaatgtagct ggcgcgaagg caataagatc cagttgctgg aaaacggcga
    41401 gcaatattat cccgcggtgt ttaaggcgat tggcgaggca caagaacgca tcattcttga
    41461 aacgtttatc tggtttgagg atgacgtcgg caaacaactg catgcggcac tactggcagc
    41521 agcgcaacgc ggggtaaaag cggaagtctt acttgatggc tacggttcgc cggatctcag
    41581 cgatgagttt gtcaatgaac tgacggccgc tggcgtggtg ttccgctact acgatccccg
    41641 ccctcgcctt tttggtatgc gcaccaatgt gtttcgccgg atgcatcgca aaattgtggt
    41701 gatcgacgcg catatcgcct ttattggcgg gctgaattac tccgccgagc atatgtccag
    41761 ctacggtcca gaggctaaac aggattacgc ggtgcgcctt gaagggccga ttgtcgaaga
    41821 tattctgcaa tttgaactgg aaaacctgcc aggacagagc gcggcacgac gctggtggcg
    41881 acgtcatcac aaagcggaag agaaccgcca gccgggagaa gcgcaggtat tgctggtctg
    41941 gcgcgataac gaagaacatc gcgatgatat tgaacgtcac tatctgaaaa tgctcactca
    42001 ggcacggcgg gaagtgatta tcgccaacgc ctacttcttc cccggctatc gatttttaca
    42061 cgccttgcgt aaagcggcac ggcgcggggt gcggatcaaa ctgatcattc agggcgaacc
    42121 ggatatgccg attgtcagag tcggcgcgcg cttgctgtat aactatctgg ttaaaggcgg
    42181 cgttcaggtt tttgagtatc gccgccgccc gctccacggc aaagtggcgt tgatggacga
    42241 tcactgggcg acggtagggt ccagtaatct cgatccgctc agtttgtcac tgaatctcga
    42301 agcaaatgtc atcatccacg atcgtcattt taaccagacg ctgcgcgata atctgaacgg
    42361 cattatcgcc gcagactgcc agcaggtgga tgaaaccatg ctgccgaaac gcacctggtg
    42421 gaatctgacc aaaagcgtgc tggccttcca ctttttacgc cacttcccgg cgctggtcgg
    42481 ctggcttccg gcacacacac cacgtctggc gcaggttgac ccgcccgctc agccgacaat
    42541 ggaaacgcag gatcgggtag aaactgaaaa cacgggggta aaaccctgat gagtaaatca
    42601 cacccacgct ggcgcttagc aaagaagatc ctcacctggc tgttttttat cgcggtgata
    42661 gtgttgctgg tggtctacgc caaaaaggtg gactgggaag aggtctggaa ggtcatccgc
    42721 gactacaatc gcgttgcgtt gctcagtgcg gtcgggctgg tggtcgtcag ttatctgatt
    42781 tacggctgct atgatctgct cgcccgcttt tactgcggtc acaaactggc gaagcgccag
    42841 gtgatgctgg tgtcgtttat ctgctacgcc ttcaacctga cgctcagtac ctgggtcggc
    42901 ggcattggta tgcgctatcg tttgtactcc cggctagggt taccgggcag cactattacg
    42961 cggattttct cgctcagtat taccaccaac tggctgggct atattttact ggcagggatt
    43021 atctttaccg caggcgtggt ggagttgccg gatcactggt atgtcgatca aaccacgctg
    43081 cgtattctcg gcattggctt attgatgatt atcgcggttt atttgtggtt ttgcgctttc
    43141 gcgaagcacc gccatatgac catcaaagga caaaagctgg tgctgccttc atggaaattt
    43201 gccctcgccc agatgctgat ttccagcgtt aactggatgg taatgggggc gattatctgg
    43261 ctgttacttg gtcaaagcgt gaactatttc tttgtactgg gcgtgttact ggttagtagt
    43321 attgctggcg tcatcgtgca tattccggcg gggattggtg tgctggaagc ggtatttatc
    43381 gcgctactgg ctggggagca tacctcgaaa gggagcatta tcgccgccct gctcgcttat
    43441 cgcgtcctgt attactttat cccgctgctg ctggcgctga tctgttacct gctactggaa
    43501 agccaggcga agaagctacg agcgaaaaat gaagcggcga tgtgaagttt agttaagttc
    43561 tttagtatgt gcatttacga ctaatgaaaa aaacgcgtat gcctttgcca gacaagcgtt
    43621 atagctatag cggcaagtat cccaaagaag ccgataaaat agtaatagag tgttaatgca
    43681 cctgtaaaag cgcaacgaac cggactccac aactcttcgc tatggcattt tcctgccagt
    43741 tcaaggagct tatttcgtcc atgcgagata atgcctgacc ataacactat cattaagcaa
    43801 cttattaccc aaacgggtcg ttcactcatt aatgcggcat tcatgatgat caccaggacc
    43861 agccctgcgg ttgccaccat acccaaagcg gtaatgagcc atataccgtt taatatcctg
    43921 gcctcgtgct gaaaatctaa tttattgctg ttttgcgagt atgattccat tcttttagtt
    43981 gtccctagaa aaaatattaa tattcaccat caataaattt aaaaccacgc atacaaaata
    44041 cttttgtatt tatggatacg gaaaacactt ccgctcggcc ataaggcata
//
LOCUS       DAPOZO010000034        43961 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPOZO010000034 DAPOZO010000000
VERSION     DAPOZO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.034"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1886)
                     /gene="aceF"
                     /locus_tag="ROI21_002004"
     CDS             complement(<1..1886)
                     /gene="aceF"
                     /locus_tag="ROI21_002004"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDX5110924.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKREAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLV"
     gene            complement(1901..4564)
                     /gene="aceE"
                     /locus_tag="ROI21_002005"
     CDS             complement(1901..4564)
                     /gene="aceE"
                     /locus_tag="ROI21_002005"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDX5110925.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(4725..5489)
                     /gene="pdhR"
                     /locus_tag="ROI21_002006"
     CDS             complement(4725..5489)
                     /gene="pdhR"
                     /locus_tag="ROI21_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDX5110926.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            6030..7403
                     /gene="aroP"
                     /locus_tag="ROI21_002007"
     CDS             6030..7403
                     /gene="aroP"
                     /locus_tag="ROI21_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDX5110927.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(7446..8300)
                     /gene="ampE"
                     /locus_tag="ROI21_002008"
     CDS             complement(7446..8300)
                     /gene="ampE"
                     /locus_tag="ROI21_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDX5110928.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRTLQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(8297..8848)
                     /gene="ampD"
                     /locus_tag="ROI21_002009"
     CDS             complement(8297..8848)
                     /gene="ampD"
                     /locus_tag="ROI21_002009"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDX5110929.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            8936..9829
                     /gene="nadC"
                     /locus_tag="ROI21_002010"
     CDS             8936..9829
                     /gene="nadC"
                     /locus_tag="ROI21_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDX5110930.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANE
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            10032..10472
                     /gene="ppdD"
                     /locus_tag="ROI21_002011"
     CDS             10032..10472
                     /gene="ppdD"
                     /locus_tag="ROI21_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDX5110931.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTNMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            10482..11867
                     /gene="gspE"
                     /locus_tag="ROI21_002012"
     CDS             10482..11867
                     /gene="gspE"
                     /locus_tag="ROI21_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDX5110932.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGF
                     YGRTALFEVLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            11857..13059
                     /gene="hofC"
                     /locus_tag="ROI21_002013"
     CDS             11857..13059
                     /gene="hofC"
                     /locus_tag="ROI21_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDX5110933.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLIALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLADKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPVLTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGKKLTQIFTILALTQSA
                     GITFLQGVESVRATMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTLALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(13094..14137)
                     /gene="guaC"
                     /locus_tag="ROI21_002014"
     CDS             complement(13094..14137)
                     /gene="guaC"
                     /locus_tag="ROI21_002014"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDX5110934.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(14293..14337)
                     /locus_tag="ROI21_002015"
     CDS             complement(14293..14337)
                     /locus_tag="ROI21_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110935.1"
                     /translation="MIIKTLKMSARKRN"
     gene            14362..14982
                     /gene="coaE"
                     /locus_tag="ROI21_002016"
     CDS             14362..14982
                     /gene="coaE"
                     /locus_tag="ROI21_002016"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDX5110936.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            14982..15725
                     /gene="zapD"
                     /locus_tag="ROI21_002017"
     CDS             14982..15725
                     /gene="zapD"
                     /locus_tag="ROI21_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDX5110937.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQTQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            15735..15932
                     /gene="yacG"
                     /locus_tag="ROI21_002018"
     CDS             15735..15932
                     /gene="yacG"
                     /locus_tag="ROI21_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDX5110938.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(16134..16532)
                     /gene="mutT"
                     /locus_tag="ROI21_002019"
     CDS             complement(16134..16532)
                     /gene="mutT"
                     /locus_tag="ROI21_002019"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDX5110939.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADEFPPANEPVIAKLKRVYVG"
     gene            complement(16592..19297)
                     /gene="secA"
                     /locus_tag="ROI21_002020"
     CDS             complement(16592..19297)
                     /gene="secA"
                     /locus_tag="ROI21_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDX5110940.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(19359..19871)
                     /gene="secM"
                     /locus_tag="ROI21_002021"
     CDS             complement(19359..19871)
                     /gene="secM"
                     /locus_tag="ROI21_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDX5110941.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(20103..21020)
                     /gene="lpxC"
                     /locus_tag="ROI21_002022"
     CDS             complement(20103..21020)
                     /gene="lpxC"
                     /locus_tag="ROI21_002022"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDX5110942.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(21121..22272)
                     /gene="ftsZ"
                     /locus_tag="ROI21_002023"
     CDS             complement(21121..22272)
                     /gene="ftsZ"
                     /locus_tag="ROI21_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDX5110943.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMERYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(22333..23595)
                     /gene="ftsA"
                     /locus_tag="ROI21_002024"
     CDS             complement(22333..23595)
                     /gene="ftsA"
                     /locus_tag="ROI21_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDX5110944.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRGTASVGSWIKRLNSWLRKEF"
     gene            complement(23592..24422)
                     /gene="ftsQ"
                     /locus_tag="ROI21_002025"
     CDS             complement(23592..24422)
                     /gene="ftsQ"
                     /locus_tag="ROI21_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDX5110945.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(24424..25344)
                     /gene="ddlB"
                     /locus_tag="ROI21_002026"
     CDS             complement(24424..25344)
                     /gene="ddlB"
                     /locus_tag="ROI21_002026"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDX5110946.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(25337..26812)
                     /gene="murC"
                     /locus_tag="ROI21_002027"
     CDS             complement(25337..26812)
                     /gene="murC"
                     /locus_tag="ROI21_002027"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDX5110947.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEAQHD"
     gene            complement(26866..27933)
                     /gene="murG"
                     /locus_tag="ROI21_002028"
     CDS             complement(26866..27933)
                     /gene="murG"
                     /locus_tag="ROI21_002028"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDX5110948.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(27930..29174)
                     /gene="ftsW"
                     /locus_tag="ROI21_002029"
     CDS             complement(27930..29174)
                     /gene="ftsW"
                     /locus_tag="ROI21_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDX5110949.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(29174..30490)
                     /gene="murD"
                     /locus_tag="ROI21_002030"
     CDS             complement(29174..30490)
                     /gene="murD"
                     /locus_tag="ROI21_002030"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDX5110950.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTATTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(30493..31575)
                     /gene="mraY"
                     /locus_tag="ROI21_002031"
     CDS             complement(30493..31575)
                     /gene="mraY"
                     /locus_tag="ROI21_002031"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDX5110951.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGIIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVSGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(31569..32927)
                     /gene="murF"
                     /locus_tag="ROI21_002032"
     CDS             complement(31569..32927)
                     /gene="murF"
                     /locus_tag="ROI21_002032"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDX5110952.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTSEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALIARIKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(32924..34411)
                     /gene="murE"
                     /locus_tag="ROI21_002033"
     CDS             complement(32924..34411)
                     /gene="murE"
                     /locus_tag="ROI21_002033"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDX5110953.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(34398..36164)
                     /gene="ftsI"
                     /locus_tag="ROI21_002034"
     CDS             complement(34398..36164)
                     /gene="ftsI"
                     /locus_tag="ROI21_002034"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDX5110954.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERVTFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(36180..36545)
                     /gene="ftsL"
                     /locus_tag="ROI21_002035"
     CDS             complement(36180..36545)
                     /gene="ftsL"
                     /locus_tag="ROI21_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDX5110955.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(36542..37483)
                     /gene="rsmH"
                     /locus_tag="ROI21_002036"
     CDS             complement(36542..37483)
                     /gene="rsmH"
                     /locus_tag="ROI21_002036"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDX5110956.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(37485..37943)
                     /gene="mraZ"
                     /locus_tag="ROI21_002037"
     CDS             complement(37485..37943)
                     /gene="mraZ"
                     /locus_tag="ROI21_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDX5110957.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(38544..39548)
                     /gene="cra"
                     /locus_tag="ROI21_002038"
     CDS             complement(38544..39548)
                     /gene="cra"
                     /locus_tag="ROI21_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDX5110958.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(39630..39716)
                     /locus_tag="ROI21_002039"
     CDS             complement(39630..39716)
                     /locus_tag="ROI21_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDX5110959.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(39728..40219)
                     /gene="ilvN"
                     /locus_tag="ROI21_002040"
     CDS             complement(39728..40219)
                     /gene="ilvN"
                     /locus_tag="ROI21_002040"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDX5110960.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(40222..41946)
                     /gene="ilvI"
                     /locus_tag="ROI21_002041"
     CDS             complement(40222..41946)
                     /gene="ilvI"
                     /locus_tag="ROI21_002041"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDX5110961.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDSYSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            42065..42109
                     /locus_tag="ROI21_002042"
     CDS             42065..42109
                     /locus_tag="ROI21_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5110962.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(42264..43208)
                     /gene="leuO"
                     /locus_tag="ROI21_002043"
     CDS             complement(42264..43208)
                     /gene="leuO"
                     /locus_tag="ROI21_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDX5110963.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERIFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            43867..43953
                     /gene="leuL"
                     /locus_tag="ROI21_002044"
     CDS             43867..43953
                     /gene="leuL"
                     /locus_tag="ROI21_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDX5110964.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRLVSGIQN"
BASE COUNT        10529 a        12154 c        10730 g        10548 t
ORIGIN      
        1 accagacggc gaatgtcaga cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca
       61 gcaccgtcga tcacgcggtg gtcgaaggag agagaaatcg gcagcatcag acgcggcacg
      121 aactctttac cattccacac cggctccatc gcggacttgg aaacgccgag gatagccact
      181 tccggcgcgt tcacaatcgg cgcgaagtgg gtagtaccca ggccgccgat gctggagatg
      241 gtgaagcaac cgccctgcat ttcgcccgca gtcagcttac cgtcacgcgc tttcttagaa
      301 atagtcatca gctcgcgaga cagctcgatg atgcctttct tgttgacgtc tttgaatacc
      361 ggaacaacca gaccgttcgg ggtatccacc gccacaccga tgttgatgta tttcttcagg
      421 gtcagacgct gaccgtcttc cgacagcgaa ctgttgaagc gaggcatctg ctcaagagct
      481 gcagcaaccg ctttcatgat gaagacaact ggggttatct tcacatccag cttacgtttc
      541 gccgcttctt cgttctgctg tttacggaac gcttccagct cggtgatatc ggttttgtcg
      601 aagtgagtaa catgcgggat catcacccag ttacggctca ggttcgcacc agaaattttc
      661 tggatacggc ccagttccac ttcttcgatt tcaccaaact tgctgaagtc caccttcggc
      721 cacggcagca tgcctgggat accgccgcca gtcgccgccg gagccgcttc tgcacgtttg
      781 atagcttctt tcacgtaagc ctgaacgtct tcgcgcagga tacgaccttt acggccagtg
      841 cccttcactt tcgccaggtt aacgccaaac tcgcgtgcca gacggcggat cagcggagtc
      901 gcgtgaacgt aagcgtcgtt ttcagcaaat tcagatttgc cttccgcttt cgcagccggt
      961 gctgctgccg gagcttcagc ttttgctgcc ggagccggcg ctgccgcttc ccgtttcgca
     1021 ggagctgccg caggcgctgc gccttcaact tcgaagatca taatcagcga gccagttttc
     1081 actttatcgc caacgttgac tttcagttcc ttcacgacgc ctgcaaacgg agccggaact
     1141 tccatagaag ctttatcgcc ttctacggtg atcagtgact gttcagcggc aactttgtcg
     1201 cccactttca ccatcacttc ggtcacttca acttcgtcac cgccgatatc cggaacgtta
     1261 acttctttca cgccagctgc tgatgcaggg gccgctgccg gagctgcttc ctgtttagcc
     1321 gccggagctg ccgcgcctgc ttcacccgcg acttcgaaga ccataatcag cgagccggta
     1381 gacactttgt cacccacgtt cactttgatc tctttcacgg tgcctgcaaa cggagccgga
     1441 acttccatag aagctttatc gccttctacg gtgatcagcg actgttcagc ttcaacttta
     1501 tcgccaactt tcaccaggat ttcggtcact tcaacttcgt cgctgccgat atccggaacg
     1561 ttaacgtctt ttgccgctgc agccgctggt gctgctgccg gagctgcttc tttcttctct
     1621 tctgcctgag caggtgcagc gtcagctgca ccgtcggcgg aatcgaaaat cataatcagt
     1681 gcgccggtct gggttttatc gccaacagag actttgatct ctttaacgat acccgcctgc
     1741 ggagacggaa cttccataga ggctttgtcg ccttctacgg tgatcagcga ctgttcggct
     1801 tcaactttgt cgcccacttt gaccaggatc tcggtgattt caacttcatc agccccgatg
     1861 tccggtactt tgatttcgat agccattatt cttttacctc ttaagccaga cgcgggttaa
     1921 ctttatctgc atcgatgttg aatttggcga ttgcgtcagc aaccactttc ttatcgattt
     1981 cgccacgttt agccagttcg cccagcgccg caaccacgac ataagaagca tcaacttcga
     2041 agtggtgacg caggttctca cggctgtcgg aacgaccgaa gccatcagta cccagtacgc
     2101 ggtagtcgtc agccggtacg taagtacgga cctgctcagc gaacagtttc atatagtcgg
     2161 tagatgccac tgccggagcg tcgttcatca cctgagcgat gtatggaacg cgcggagttt
     2221 ccagcgggtg cagcatgttc cagcgttcac aatcctgacc atcacgcgcc agctcggtga
     2281 aggaggtcac gctataaacg tcagaaccta cgccgtaatc tttcgccagg atctctgccg
     2341 cttcacggac gtgacgcagg atagaaccgg agcccagcag ctgaacttta cctttgctac
     2401 cttcaatggt ttcgagtttg tagatacctt tacggatacc ttcctcagca ccttccggca
     2461 ttgccggcat gtggtagttt tcgttcagcg tagtgatgta gtagtaaacg ttctcttgtt
     2521 tttcaccgta catacgctcc agaccgtcat gcatgatgac agcaacttcg taagcgtaag
     2581 ccgggtcgta agagatacag ttcgggatag tcagcgactg aatgtggctg tgaccatctt
     2641 cgtgctgcag accttcgccg ttcagggtgg tacgaccgga agtaccgccg atcaggaagc
     2701 cacgcgcttg ctggtcgcca gccgcccagc acaagtcacc gatacgctgg aagccgaaca
     2761 tcgagtaata gatgtagaac ggaatcatcg gcagattgtt ggtgctgtaa gaggtcgccg
     2821 ctgccagcca ggaacaccct gcgcccagct cgttgatccc ttcctgcaga atctgacctt
     2881 tctcgtcttc tttatagtaa gcaacctgct cgcggtcctg cggggtgtac tgctgaccgt
     2941 tcgggctgta aataccaatc tgacggaaca gaccttccat accgaaagta cgcgcttcgt
     3001 cggcgatgat cggtaccaga cgatctttga tcgacttgtt cttcagcatc acgttcaggg
     3061 cacgaacgaa agcgatagtg gtagagatct ctttgctctg ctcttccagc agcgcgccga
     3121 agtcttgcag gctcggcagc tcaagcttct cggtgaagtt cggctgacgg cttggcagat
     3181 aaccgtgcag tttctgacgc tgagcgtgca gataggtatg ctcttcagaa ccttccggga
     3241 aggtgatgta cggcagtttt tcgatatcgg catcagacac cggcacattg aaacggtcgc
     3301 ggatatgacg cacgccgtcc atgttcattt tcttaacctg gtgcgcgatg tttttacctt
     3361 cagccgcgtc gcccatgccg taacctttaa tggtatgagc aaggattacc gtcgctttgc
     3421 ctttggtttc ctgcgctttc ttgaatgcag cgtagatttt cttcggatcg tgaccaccac
     3481 ggttcagcgc ccagatctgc tcgtcagtcc agtctgcaac cagtgctgcg gtttcaggat
     3541 atttaccgaa gaagtgttca cgaacgtacg caccatcttt cgatttgaag gtctggtagt
     3601 cgccgtcaac ggtttcgttc atcagctgga tcagtttacc gctggtatct ttacgcagca
     3661 gttcatccca acggctaccc cacatcactt taatcacgtt ccagccagca ccttcgaaga
     3721 tgccttccag ttcgttgatg atcttgccgt taccggtgac cgggccgtca agacgctgca
     3781 ggttacagtt gataacgaag accaggttat ccagtttttc acgggtagcg atggtgatcg
     3841 cacctttgga ttccggttcg tccatttcac cgtcgccgag gaacgcgtaa acggtctgtt
     3901 tagaggtatc tttcaggcca cggtgttcca gatatttcag gaatttagcc tggtaaatag
     3961 caccaatcgg ccccagcccc atagatacgg tcgggaactg ccagaattcc ggcatcagtt
     4021 tcgggtgcgg ataggaagag aggccattgc cgtgaacttc ctgacggaag ttatccagct
     4081 gctcctgagt cagacgacct tccaggaaag cacgagcgta aacgcccggg gagatgtggc
     4141 cctggaagta aaccaggtcg ccgccatcct gctcgttgcg tgcacggaag aagtggttaa
     4201 agcacacatc ataaatggtt gcggaagact ggaaggacgc catgtggccg cccagttcga
     4261 ggtctttttt cgacgcacgc agaaccgtca tgatggcgtt ccagcggata gctgaacgaa
     4321 tacggcgttc cagttccaga ttacccggat actccggttg ttcttcaacg gggatggtgt
     4381 tgatgtagtt gctgataccg gtgcctgcgg ctacgttgac accgcctttg cgggcttcag
     4441 caagcagttg gtcgatcaga tactgagcac gctcaacacc ttcttcacgg atgaccgatt
     4501 cgatcgcctg gagccagtcg cgagtttcga tcggatccac gtcatttggg aaacgttctg
     4561 acatgggtta ttccttatct atctaataac gttgagtttt ctggaacctg tcccattgaa
     4621 ctctcgccgg aaagctcaat aagacaggtt ctacgtttag ttgccgcgct tttatatgcg
     4681 cttgatttac aacatcttct ggttaatttt taccagaaaa atcactaatt ctttcgttgc
     4741 tccagacgac gtagagaacg ctcacggcgg ctctcttcac ggcttctgtc gagcaaaatt
     4801 tcttcgataa aggccagatg gcgatgcgat gcttcgcgcg cttcttccgg cttaccggcc
     4861 ataatcgctt cgaatatgcg ggtgcggtga ctactcacca gcggcagcat ctcgcgacgc
     4921 gaatagagca attcgaagtt ctggcggaca ttctgagcca acatcggctc catacacctt
     4981 agcagatgaa gcagaaccac attgtgggcc gcttcggtga cggcaatctg atactggagt
     5041 acggcgtttg attccgcgtc cagatcgcca gactgctgcg ccagctctat agcgtggtgg
     5101 agttcacgga tgcgttcctt gtcttcatcg gtactacgca gcgcggcgta ataagcggcg
     5161 ataccttcca gggcgtgtcg tgtttcgagc aagtcatact gtgattcagg atggtcggag
     5221 agcagctcca ccagcggatc gctgaagctt tgccataggc tgctctggac aaaagtgccg
     5281 ccaccctggc gacgaagcaa caagcccttc gcttcgagac gttgaatcgc ctcacgcaag
     5341 gagggacggg agacgtcaaa ctgttttgcc agttcgcgtt ccggtgggag tttttcgccc
     5401 gggcggagag tgccttcgag gatcaaaaac tccagttgct gctcaatcac atcggagagt
     5461 tttggttggc ggattttgct gtaggccatg agttcctgtc ttaagccact tgccgaagtc
     5521 aattggtctt accaatttca tgtctgtgac gctaaagtaa caaagtattc accttatgtc
     5581 catacaggtt ttgattgaaa tcatgaaact gtgcacattt taacaacttg acatatataa
     5641 cgtttcaaag ttgtaactat gcacaaatgt agactttacg taggaaaggg attttttaac
     5701 gattattaaa ctataaaaat ccgaattgaa ccgattcact taccaattta tcgattatta
     5761 attcaaaaat aatgaattta tattcatttt gtatattgag gggatttaag gctgaccttt
     5821 acaaacggtt cctttttaag caactcatct tcaaccatgc ataaagcggg tgcattcgct
     5881 gccgcatacc attattcttg atctgacgga agtccttttg taacaattca aacgtctttg
     5941 atgtaaacaa attaatacaa caaacggaat tgcaaactta cacacgcatc actgcgtaga
     6001 tcaaaaaaac aatcaccgaa cgaggtttca tgatggaagg tcaacagcac ggcgagcagc
     6061 taaagcgcgg ccttaaaaac cgccatattc agcttatcgc gctgggtggc gcgataggga
     6121 ccgggctatt cctgggtagc gcctccgtaa tacagtccgc agggccaggg attatcctgg
     6181 gttacgccat tgctggtttt atcgcctttc tgatcatgcg tcagctaggt gaaatggtgg
     6241 tcgaagaacc tgtcgcaggc tcctttagcc actttgctta taaatactgg ggcagttttg
     6301 ccggtttcgc ctctggctgg aactactggg tactgtacgt tttagttgcc atggcagagc
     6361 tgactgccgt gggtaaatac attcagttct ggtatccgga aatcccaacc tgggtttctg
     6421 ccgccgtgtt ctttgtggtg attaacgcca tcaacctgac caacgttaaa gtgtttggcg
     6481 agatggagtt ctggtttgcc attatcaaag ttatcgcggt ggtagcgatg atcatcttcg
     6541 gcggctggct gctattcagt ggcaacggcg gcccgcaggc gagcgttagc aacctgtggg
     6601 atcagggtgg tttcctgccg cacggcttta ccgggctggt gatgatgatg gcgattatca
     6661 tgttctcgtt cggtggtctg gaactggtgg ggatcaccgc agcagaagct gataacccgg
     6721 agcaaagtat cccgaaagca actaaccagg ttatctaccg catcctgatt ttctatattg
     6781 gttcgttagc cgttctgctc tcgctgatgc cgtggacccg cgttactgct gataccagcc
     6841 cgtttgtact gatcttccac gaattgggcg atacctttgt ggcgaatgcg ctgaacatcg
     6901 tggtactgac tgctgcgctc tccgtataca acagctgcgt atattgcaac agccgtatgc
     6961 tgtttggtct ggcacagcag ggcaacgcgc caaaagcgct ggcgtctgtc gacaaacgcg
     7021 gtgtaccggt taacactatt ctggtgtctg cgctggttac ggcattgtgc gtgctgatta
     7081 actaccttgc accggaatcc gcattcggac tgttaatggc gctggtggta tctgcactgg
     7141 taatcaactg ggcgatgatt agcctggcgc atatgaaatt ccgtcgcgcc aagcaggaac
     7201 aaggcgtggt aactcgcttc cctgctctgc tttatccgct gggtaactgg atctgcctgc
     7261 tgtttatggc ggcggtactg gtgattatgc tgatgacccc aggaatggcg atttcggtat
     7321 acctgatccc ggtatggctg atcgtgttag gtatcggcta tctgtttaaa gagaaaaccg
     7381 ccaaagccgt aaaagcgcat taatctctct acgccctcac ccgtacaggg tgagggcaat
     7441 aatctttaca ccaacgcccc gtaaatcgtc agtagcgcaa tcaccaccac gaccacgaac
     7501 gaggttttct tcgccatgga aaccgctgcc ttcggcgtct ccaccttatc gacatgcggt
     7561 tcacgcgcca gagagaactg tgccagacgc gttaatacct gatattgcga agtatggaaa
     7621 tcacccagcg aagcaaacca ggccggtaac gctttctccc catgaccgat caaggcatat
     7681 accacacccg caagacgaac cggcacccaa tccagtacat gaagcactgc atcgatgccg
     7741 gactgtaaac gatgatgcgg cgtctggtat cgcgccagcc agtattgcca tgcacgcaaa
     7801 aacgcatacc ccatcagcgt aacgggtccc caggttcccc ccacaatcag ccagaacaac
     7861 ggagcaagat aaaaacgaaa gttaatccac agcaatgcat tttgcagctc acgcaaatac
     7921 tcacgttcat cgcagcctgc cgggacgccg tgaatcatgg tgagttcgcc agccatcgtg
     7981 gcacgggcat ggctatcatt acgtgaagca gctgtcagat aagcatgata atgaagacga
     8041 actttacctg cgccaataca cagcaaacca atcagcagcc acaccagcag cgtgggaacg
     8101 ttgaataata ctccctgcaa tgtgcgtaac agtaaaaaag tcacgcccat cgcaataatg
     8161 gtcatgccta atgtgcgccc gagagaaaaa tgtttcaccc gccgaaagaa cgcttcaaga
     8221 cgatgatcaa gctgccagtg ctcgcccaac ttaaacaggc gctcgaaaat taacaccagt
     8281 aaggttgtaa atagcgtcat gttgtctcct tgctgaccag tgcacgaaac cgtgcccaat
     8341 caaatgcagg accgggatcg gttttccgat ccggcgcaat atcacaatgg cccgtcatgt
     8401 tattagcgat atctggatag cgatcaatca gtgcccgcgt aaccgccgca agctgttgat
     8461 actgcgcatc ggtatacgcc agcgtatcgg tgccttccag ctcaatccca atagaaaaat
     8521 cattgcagcg ttcgcgcccc tgatactgag agactcccgc atgccaggca cgtttatcga
     8581 aaggaacata ctggactatt tcaccatcac ggcgaatcaa acagtgagcg gagacgcgca
     8641 aatgggcgat ctcagcaaag aaaggatgtg cctgcggatc aatagttcca gtgaataatg
     8701 cgtcgatcca cggaccgcca aactcgcctg gcggcaggct aatattgtgc accaccagca
     8761 gggtgggtgt ttcgtcatcc gggcggcaat cgtaatgtgg tgagggaacg cggcgcgcgc
     8821 caaccagcca cccctgttct aacaacatgc aggatctcct tatatgtggt gctaataccc
     8881 ggttcagagt agcatgtttc taccttatga ttcgttagct atctggagtt ttaacatgcc
     8941 gcctcgccgc tataaccctg acacccgacg tgacgagctg ctggaacgca ttaatctcga
     9001 tatccccggc gcggtggccc aggcgctacg ggaagattta ggcggaacag tcgatgccaa
     9061 caatgacatt acggcaaaac ttttaccgga aaactctcgc tctcatgcca cagtgatcac
     9121 ccgcgagaat ggcgtatttt gcggcaaacg ctgggttgaa gaggtgttta ttcaactggc
     9181 aggcgacgat gtcaccataa tctggcatgt ggatgacggc gatgtcatca acgccaatga
     9241 acccttgttc gaacttgaag gcccatcccg cgtgctgtta acgggcgaac gcactgcgct
     9301 taattttgtg caaacccttt caggggttgc cagtaaggta cgccactatg tcgaattact
     9361 ggagggcacc aacacgcagt tgttggatac gcgcaaaacc ttacccggcc tgcgttcagc
     9421 tctgaaatac gcggtacttt gcggcggcgg agcgaatcac cgcctggggc tttctgatgc
     9481 cttcctgatc aaagaaaacc atattattgc ctccggctca gtgcgccagg cggtcgaaaa
     9541 agcatcctgg ctgcacccgg atgcgccagt agaagtcgaa gtagagaatc tggaagaact
     9601 tgatgaagcc ctgaaagcag gtgccgatat catcatgctg gataacttcg aaacagaaca
     9661 gatgcgcgaa gccgtcaaac gcaccaacgg caaggcgcta ctggaagtgt ctggcaacgt
     9721 cactgacaaa acactgcgcg aatttgccga aacgggcgtg gactttatct ccgtcggtgc
     9781 gctaactaaa cacgtacagg cactcgacct atcaatgcgt tttcgctgat tgctgacaga
     9841 tttatccgct ccatcgcggg cggataatct cccctttccc aactttcttc gtaacgcctc
     9901 gcaaatttct tgtctttcgc tgcaacgtaa taaaaaactc cggaagctgc tttcccgttt
     9961 cgttttttgc ccctcgcttg tgctgatggc gtttgccaaa gtagcgccaa ccaaatcaag
    10021 gagcgaaaca aatggacaag caacgcggtt ttacacttat cgaactgatg gtggttattg
    10081 gcatcattgc cattttaagc gccattggta ttcccgctta tcaaaactac ctgcgcaaag
    10141 ccgcactcac caacatgcta caaacctttg tgccttaccg taccgccgta gagttgtgcg
    10201 cgctggaaca tggtggatta gatacctgcg acggtggcag taatggcatc ccctcgccta
    10261 ccactacccg ctatgtttca gccatgagtg tggcaaaggg cgtggtgtcg ctgaccgggc
    10321 aagaaagtct caatgggcta agcgtcgtca tgacgccggg ttgggataac gcaaacggcg
    10381 tcaccggttg gacgcgcaac tgcaatattc aaagtgacag cgcattgcag caagcctgcg
    10441 aagatgtctt tcgctttgat gacgcaaact aaggagcggc aatgaatatt ccacagctca
    10501 ccgccctgtg tctgcgttat cagggagtct tgctggatgc cagcgaagag gtggttcatg
    10561 ttgcggtagt cgatgcacct tcgcatgagc tactggacgc attgcatttc gctaccacca
    10621 aacgtattga gatcacctgc tggacgcgcc aacaaatgga aggtcacgcc agtcgcacac
    10681 aacagacatt gcccgtagct attcatgaga agcatcagcc caaagcagag ttgctgactc
    10741 gaacgttaca atctgcgctg gaacaacgcg cgtctgatat tcatatcgaa ccagcggaca
    10801 atgcctaccg catacgcttg cgtatcgacg gcgtattgca tcctttaccg gatgtttcac
    10861 cggatgccgg agtcgcatta accgccagat taaaagtgct ggggaacctg gatattgcgg
    10921 aacatcgcct gccgcaggac gggcaattca ctgtcgaact ggcaggaaac gccgtctcat
    10981 ttcgtattgc gaccttacca tgtcggggtg gtgaaaaggt ggtattaagg ttgttacagc
    11041 aggtgagtca ggcactggat gtcaacacgc ttggaatgca gccgttacaa ctggcggact
    11101 ttgcccatgc cttgcaacaa ccacagggac tggtgctggt aactggccct acaggcagcg
    11161 gcaaaacggt cacgctttat agtgccctgc aaacgctgaa taccgctgac attaatattt
    11221 gtagcgtcga agatccggtt gagatcccca tagccggact aaaccagacg caaatccatt
    11281 cgcgtgccgg actcaccttt cagggcgttt tgcgtgcgtt attgcgccag gaccctgacg
    11341 tcatcatgat cggagagatc cgcgatggcg aaacggcaga aattgccatt aaagccgcgc
    11401 aaaccggtca cctggtgttg tctaccctac acactaattc cacctgcgaa acgctggtac
    11461 gtttacagca aatgggagtc gcccgctgga tgctatcatc ggcgcttacg ctggtaatag
    11521 cccagcgtct ggtacgcaaa ctttgcccgc attgtcgccg gcagcaaggg gagcccatcc
    11581 atattccaga caatgtatgg ccgtcgccgc tgccccgctg gcaagcaccc ggttgtgtac
    11641 attgctacca cggtttttat ggtcgtacgg ccttatttga agttctaccc ataacaccga
    11701 tcatacgcca gcttatttcc gctaataccg acgttgaatc gctggaaaca cacgcccgac
    11761 aggcgggtat gcgtacgctt tttgaaaacg gctgcctggc cgtggagcaa ggcttaacca
    11821 cctttgaaga gttaatccgc gtattgggga tgccgcatgg cgagtaagca actctggcgc
    11881 tggcatggca taaccggcga cggcaatgcg caagatggga tgctatgggc tgagagccgt
    11941 gctttgctgc tcatagcact acagcaacag atggttaccc cacttagcct gaagcgaatc
    12001 gccatcaatt ctgcgcagtg gcgaggagat aaaagcgcgg aagtcattca tcaactggcg
    12061 acgctactca aagccgggtt aacgctttct gaagggctgg cactgctggc ggaacagcat
    12121 cccagtaagc aatggcaagc gttgctgcaa tcgctggcgc acgatctcga acagggcatt
    12181 gctttttcca atgccttatt accctggtca gaggtatttc cgccgctcta tcaggcaatg
    12241 atccgcacgg gggaattgac tggtaagtta gatgaatgtt gctttgaact tgctcgacag
    12301 caaaaagccc agcgtcagtt agccgataaa gtgaaatcag cgttgcgtta tcccatcatc
    12361 attttagcga tggcaatcat ggtggttgtg gcaatgctgc attttgtgct gccggagttt
    12421 gctgctattt ataagacctt caacacccca ctaccggtac taacgcaggg gatcatgacg
    12481 ctggcagact ttagtggcga atggggctgg ctgctggtgt tgttcggctt tctgctggcg
    12541 atagccaata agttgctgat gcgccgcccg acctggctta tcgcgcggca gaaattgctg
    12601 ttacgcatcc cgattatggg ttcactgatg cggggaaaaa aactcacgca gatctttacg
    12661 attctggcgc tgactcaaag tgcaggcatt acttttttac agggcgtaga gagcgtcaga
    12721 gcaacaatgc gctgcccgta ctgggtgcaa cttctgacac aaatccagca cgatatcagt
    12781 aacggtcatc ccatctggct ggcgctaaaa aataccggtg agtttagccc gctctgtttg
    12841 caattagtga gaacaggaga ggcatccggc tcgctggatc tcatgttaga caacctcgcc
    12901 catcatcatc gggataacac actggcgctg gcggataacc tcgcagcctt actggaaccg
    12961 gcgttgctga tcataacggg aggaattatc ggtacgctgg tggtggcaat gtatctgccc
    13021 attttccatt taggcgatgc aatgagtgga atgggataac gctggcgtgt tgctccacgc
    13081 cagcgttggg agattacagg ttgttgaaga tgcggttttc ttgttccagc acacggataa
    13141 atgtggtgcg cttggtcagc tctttcaggc gtgaagcccc aacgtatgta caagctgaac
    13201 gcaggccgcc caaaatatca cgcgcggtat tttcaaccgg gcctcgcagc ggcagcttaa
    13261 cggttttacc ttctgctgcg cgatattccg caacgccgcc aacgtgacgt ttcatcgcag
    13321 actcggagct catgccgtag aacagcataa atttctcgcc gttctcctca acgatgcgac
    13381 cgccgctctc ttcgtggccc gccagcatgc cgccaagcat gacgaaatcg gcaccgccgc
    13441 caaaggcttt cgcgacatcg cccggcgtgg tgcagccacc gtcgctgacg atcattccgc
    13501 ccagaccgtg cgcagcatcg gcacattcga ttaccgcaga aagttgcgga taaccgacgc
    13561 ctgttttgac gcgagttgta caaacagaac ctgggccaat gccaacttta acgatatcgg
    13621 caccggagag gataagctcc tcacacattt caccagtcac tacgttgcca gcacaaatgg
    13681 ttttggtcgg ccacgcttca cgcgctttcg caacgaactg cacgaagtgt tcggaataac
    13741 cattcgccac gtcaatacaa acgaagttta atgccgggtt caggtcgaga atctgtttag
    13801 ttttttcgaa atctgcatca gacgtgccgg tagaaaccat cacatgtttc agcacatcag
    13861 cggaagaatt gttgataaac gcttgccact cttcgacaga atagtgttta tgcacagcag
    13921 tcaaaatatc aaaagaagcc agcgcagagg ccatagaaaa tgtgcctacg gtgtccatat
    13981 ttgcggcgat aatcggcacg ccggaccagc tctgacctga atgtttgaag gtgaattgac
    14041 gttccagttc aacatcggaa cggcttttaa gagtggagcg tttagggcgg atgagaacgt
    14101 ctttaaaacc taacttcaga tcttcttcaa tacgcatgtg cggattcctg gggttaatgg
    14161 ctataagggt aagagcgcaa ctccagtgac gctatcatac gcactaatca atgcggcgca
    14221 agactgcgaa attgtctttt tttacgctac aatcccataa atttacctgg taaatagtga
    14281 gcgaacgttt catcaatttc gctttcgtgc gctcattttt aacgttttga taatcatgat
    14341 gaattcccgg aaatataaat tatgaggtat atagttgcct taacgggagg cattggcagt
    14401 ggcaagagta ccgttgccaa tgcgtttgct gatctcggaa ttaacgtcat tgatgccgat
    14461 attattgcgc gtcaggtggt tgaaccaggt gcacctgcgc tacatgccat tgctgatcac
    14521 tttggcgcta acatgattgc tgctgatgga acattgcagc gccgggcctt gcgcgagcgg
    14581 atcttcgcca acccggaaga gaaaaactgg cttaacgccc tgctgcatcc gctgattcag
    14641 caagagacgc aacaccagat ccagcaagct acttccccct atgtactatg ggttgtgcca
    14701 ttgctggtag aaaactcact gtataaaaaa gcgaatcgag tgctggtggt ggatgtcagc
    14761 ccagaaacgc aacttaagcg caccatgcag cgcgatgatg taactcgcga gcatgttgaa
    14821 caaatccttg ctgctcaggc aacgcgcgaa gcccgccttg ccgtggcaga tgacgtcatt
    14881 gataataacg gcgcaccgga tgctatcgca tcggatgttg cccgcctgca cgcacactat
    14941 ttgcagcttg cgtcgcagtt tgtctcacag gaaaaaccgt aatgcagacc caggtccttt
    15001 ttgaacatcc actaaatgaa aaaatgcgta catggctgcg cattgagttt ttgattcagc
    15061 aactcaccgt taatctaccc atcgttgacc acgctggcgc gctgcatttc ttccgtaatg
    15121 tcagtgaatt actggatgtt ttcgagcgcg gcgaagtccg cactgagctg ttgaaagaac
    15181 ttgaccggca gcaacgtaaa ctccagacct ggattggcgt gcctggcgtg gaccagagcc
    15241 gtattgaagc attaattcag cagttaaaag cggcggggag cgtattaatt tccgcgccgc
    15301 gtatcgggca atttctgcgt gaagatcgtt tgattgctct ggtgcgtcag cgactgagca
    15361 tcccaggcgg ctgttgcagc tttgatttac ctacattgca catttggctg catctacccc
    15421 agacgcagcg cgacagccag gtagaaacct ggattgccag cctgaacccg ctcacccagg
    15481 cacttaccat ggtgctggat ttaattcgcc agtcggcccc cttccgtaaa caaaccagcc
    15541 tgaatggttt ttatcaggat aacggtggcg atgccgactt gctgcgtctg aatctgtcgc
    15601 tcgattcaca gctttatccg caaatttccg gtcataagag ccgttttgcc attcgtttta
    15661 tgccgctgga cagtgaaaac ggacaggtac cggaacgtct ggatttcgaa ctggcctgtt
    15721 gctaaggagt aaaaatgtca gaaactatta cggtgaattg cccaacctgc gggaaaacgg
    15781 tggtgtgggg tgaaatcagc ccgtttcggc cattttgttc caaacgttgc cagctgatcg
    15841 acctcggaga atgggctgct gaagaaaaac gaatccccag cagtggcgat ctttccgaaa
    15901 gcgatgactg gagcgaagag ccaaagcagt gacattttgt ctgatgcgac gctggcctga
    15961 taagacgcgg gcagcgtcgc atcaggcatc gtgtgcaaat gtcggatgcg atgtgaacgc
    16021 cttatccgac ctacaagacc ttacatatca acgtgttaaa attgtagata ctgataagac
    16081 gcgccagcgt cgcatcgggc atcttgtgca aatgttggat gcggcataaa cacctatcca
    16141 acgtacacgc gcttaagctt cgcaattacc ggttcattgg ctggcggaaa ctcatcggca
    16201 ttaagaccga ccagcgacat ccacttaccg ggttgccctt ctttacccca cggctcccct
    16261 tcccagcttt cgaccagcca gaaccacagt gttatatgcc tgtccgggaa ttcatattcc
    16321 agtttttcaa atagcgaaaa atgttgcggg gtaatcccga cttcctcctg aagttcacgc
    16381 accaccgcct gttccggcgt ttcgcccatt tcaattttac cgccgggaaa ctccagttta
    16441 ttcgccatgt gcgcatctgc tgcgcgacgg gttataaaga tttcattgtt ctcgttgcga
    16501 ataataccta ccgcaatttg cagctttttc attgtcttaa acctataaaa aaggcgcaga
    16561 atcctgcgcc ttttacttca actgttagct tttattgcag gcgaccatgg cactgcttgt
    16621 attttttacc agaaccgcac gggcaaggat cgttacgtcc tactttgcgc tcaccggttt
    16681 gcgccgccag tgcagctgca gctgcagagt cgtcatcctg atggctaagc tgctgcatct
    16741 gcgctaaacg ctcggcttcc atacgacgct gttgttccag ctcctcaacc tcttcaggca
    16801 tacgtacctg aactttgctc agcgtactga taacttcata tttcaacgac tccagcatcg
    16861 ctgcaaacat ggagaacgat tcacgtttgt attcctgctt cggatctttc tgtgcatagc
    16921 cacgcaggtg gataccctga cgcagatagt ccatcgccgc caggtgctct ttccacagag
    16981 agtcaagcgt ttgcagcatg acgccttttt cgaagtgacg catcatctca gcaccaacca
    17041 cttcttcttt acgctgatac acttcgatgg actgcgccag aatgcgctca cgcagcgtct
    17101 cttcatgcag ttctggttct ttatccagcc actcggcaat tggcaaatcg aggtcgaaat
    17161 cgttcttcag acgttcctgc agccccggaa tatcccacat ttcttccagc gactgtggtg
    17221 gaatgtaggc atcaatggtc gctttgaaca catcttcacg aatgctgttg atggtttcgc
    17281 tcacatcgct gacatccagc agttcgttac gctgggagta aatggcgcga cgctgatcgt
    17341 tagccacgtc atcatattcc agcagttgct tacgaatgtc gaagttacga ctttcaactt
    17401 tacgctgggc gttggcaatc gctttagtca cccacgggtg ttcaatggct tcgcctggct
    17461 tcatacccag tttacgcatc atgccggata ctcggtcgga agcaaaaata cgcatcagcg
    17521 catcttccat cgacaggtag aaacgggaag aaccagcatc cccctgacga ccagaacgac
    17581 cgcgcaactg gttatcgata cgacgggatt cgtgacgctc ggtaccgatg atatgcaggc
    17641 cacctgctgc cagtaccgca tcgtgacgta cctgccagtc ggctttaatt ttttcaattt
    17701 gctctgcggt cggattttcc agcgcggcaa cttctgcctg ccagctacca ccgagcacaa
    17761 tatctgtacc acgacccgcc atgttggtcg cgatagtcac cgcagccgga taacctgcct
    17821 gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt caggacgttg tgcttaatac
    17881 cggctttggt cagttcgttt gacaccagct cagatttttc gatggagata gtacccacca
    17941 gcaccggctg gcctttcgca gtacgttctt tgatatcttc aatgatcgcc tgaatttttt
    18001 ctgcttcagt catgtagacc aggtccggca gatctttacg aatcattgga cggttggtcg
    18061 gaacaacgac ggtatccagc ttatagatgg agctgaattc gaaagcttcg gtatcagcag
    18121 taccagtcat ccccgccagt ttttcataca gacggaagta gttctggaag gtgatcgacg
    18181 ccagcgtttg gttttcgttc tggatctgca caccttcttt cgcttccaca gcctggtgca
    18241 gaccatcgga ccagcgacgc ccctgcatgg tacgaccggt gtgttcgtca acgatgataa
    18301 cttcaccatc tttaacgatg taatcgacgt cgcgggtaaa cagcgcatga gcgcgcagcg
    18361 ccgccgttac gtggtgcatc agcatgatgt ttgccggaga gtacagagac tccccttcat
    18421 ccatgatgcc ttctttcacc agcagttctt caatcaggac cagaccacgt tcggtcaggt
    18481 tcacctgacg agatttttcg tccaccgaga agtggccttc gccctggaag gtttcggagt
    18541 cttctttttc ctgacggatc aggtgcggaa taattttatt cacgcgttta tacatttccg
    18601 agctgtcttc tgccgggccg gaaatgatca gcggtgtacg agcttcatcg atcaggatgg
    18661 agtccacttc gtccaccagc gcatagtgca gtttacgctg tacacgttct tcagggctga
    18721 acgccatgtt atcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg taagtgatgt
    18781 cagctgcgta agcttcacgc ttcgccggtg ctggcatgcc cggcaggttg ataccgacag
    18841 tcaggccaag gaattcaaac agcggacggt tgttttcggc gtcacgttgc gccaggtagt
    18901 cgttgacggt aacaacgtgt acgcccttac cggttaatgc gttcaggtaa gcaggcagcg
    18961 ttgcggtcag ggttttacct tcaccggtac gcatttcggc gatgcagcgt tcgttaagaa
    19021 ccataccgcc gagtaactga acgtcgaagt gacgcatacc aaagacgcgc ttacttgcct
    19081 cacgcaccac ggcgaaagct tccgggatca gattttccag cacttcgcct ttttccagac
    19141 gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc ggagagtttt tccatctccg
    19201 gctccatggc attgatgatg ttaaccactt tgcgcatccg gcgcagggtg cgatcgttac
    19261 gactaccgaa aactttagtt aataatttga ttagcataat aaaatctcaa acgccccgcg
    19321 ttgcggagtc aataaaatga agtaaaggtt tattgttgtt agctgaggcg ttgagggcca
    19381 gcacggatgc cttgcgcctg gcttatccag acgggcgtgc tgaattttgc ttgcggggta
    19441 aaatgcgcat aatcaatgcg ataacccttt tcagacggcg tgccttcctg ggtcaacagc
    19501 gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga ttcttcagca
    19561 acgggcagcg tttgcggtgc cattgcgaag gaaagatgac ggattaccgt gcgaatggca
    19621 tgttgatgcc agtaatcaac ggaatagttc gaattcgggc ggcgcgtgtt cgcttccagc
    19681 aaggccaatt gaccaaagtt aactttggct gaaggctcgt ggttgcgggt tgtcgctttt
    19741 gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca aacctaaact cgccgcgacc
    19801 atccctaata agagatgcgg ccagaagtag cgtttaccaa actgtcgcca gcgcgtcagt
    19861 attccactca cgttattgcc atcccgatcg tatttaaacg tatttagcgc acaaatctta
    19921 tcattaaagc ctgaagtcca cagcacgaat gaagataagt tatgcgtaaa agtgcttaag
    19981 aaaacagcgt tcgcaccgtt ttttatcgtt cttataaaat tcaaaaaaaa aacgactggt
    20041 tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca gtataaatgt
    20101 cgttatgcca gtacggctga aggcgctttg aaggccaacg gcagttctgc gtcgtcctgg
    20161 aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag tttgttattc
    20221 agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc acacatgaac
    20281 aagtcaccga tcgcatcaag cattttgtga cgcacaaatt cgtcttcaaa acgcaggccg
    20341 tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct gccgcccagg
    20401 cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt acgcgcacgg
    20461 ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg gttgctggaa
    20521 tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt gtacggctta
    20581 aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg aacaaatttc
    20641 ttggcgcagt tcaactcgtc gataccggcg tcaagcagca gatatacaaa cggagcggcg
    20701 ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt atcgatgccc
    20761 aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc atgctcgttg
    20821 accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg gaaatctacc
    20881 ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc agggcgtaac
    20941 gtcagggtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac gatacgttta
    21001 agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac cgaagtgtac
    21061 tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc cagtcaattc
    21121 ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct ctttcgcagt
    21181 ttgcggcgca ttgtcattca caactttagc aaccggcttc tgctcctggg tcagcggagc
    21241 catcccgtgc tgctggtagc gttccatcac tggctgctga acctgcttat tggtcaccag
    21301 agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacagtta cacgcagctc
    21361 gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt cggaagcaaa
    21421 tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga agcccgctgt
    21481 gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttcaagca gcggagaaga
    21541 gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc cagaacccat
    21601 cattgcgtag cccatttcag acattacggt gcgtacgtct gcaaagtcca cgttcatcaa
    21661 acccggacga gtaatcagtt cggcgatacc ttgcacagcg cctttgagta catcgttcgc
    21721 tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca gtttgtcgtt
    21781 cgggatagtg atcagagagt ccacatgttt ggacagttca gtgatcccct gctccgcgaa
    21841 tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa cggtcaggat
    21901 acccaaatct tttgccactt cagcgacgac tggagctgcg cctgtaccgg taccaccacc
    21961 catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca gtgcatcgcg
    22021 atcctcatca gccgcattac ggccaacttc tggattagcg ccagcgccca gtcctttggt
    22081 gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg cttgtgcatc
    22141 ggtatttacc gcgaagaatt caacaccttc aatgcgctcg cgcaccatgt gttcaacagc
    22201 attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg taagttccat
    22261 tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta atcatcgtcg
    22321 gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga tccacgagcc
    22381 aactgatgct gtaccacgtt tttctacttc agcttcaccg ttaagatgtg actcttttcc
    22441 atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat ccgttaaacc
    22501 ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct gagcacaggc
    22561 tgcaagacct tcgatctgcg ctgcgccacc agttaataca atgcctgccg ccaggtgatg
    22621 tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt tgaccaggtt
    22681 gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt gcagactccg
    22741 cggcggacga ccgcctacgc tcggcacttc cacgctctca tcttttccaa cgatggaacc
    22801 cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg tgccaaaggc
    22861 gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag tgtggcgcaa
    22921 tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga cgacgcagac
    22981 acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc cagcaaatat
    23041 cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt tcgccatatc
    23101 gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa gtcctaccgg
    23161 attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat gcagcacacg
    23221 gtgttcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca catcttcttg
    23281 cgttacttct tcttcagaaa taggcaccat accaatttca ttctggcagc tgatatgctt
    23341 accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt ctgcctggtc
    23401 aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc ctttatccat
    23461 accacgtgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg gcagaacttc
    23521 ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca gttttctgtc
    23581 cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt agattcctct
    23641 ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc aacgtagcta
    23701 atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc tacaaagcga
    23761 gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt attcagcgtc
    23821 aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct gtcctttgcc
    23881 agcatctgcc ccatttcgcg atagccctgc aacacttcat tggcgctgcc ttccgggcca
    23941 tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa agtgtttcct
    24001 tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc aaccagatga
    24061 atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca cggcaggcgt
    24121 tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc tggctcaccc
    24181 aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc accggtcaac
    24241 accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac gacccagccg
    24301 ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag acgcgttcca
    24361 ttattgcggc gagaagaaac ctcttcttcg ctgtttcgcg tgttcagagc agcctgcgac
    24421 atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca tacctgcctg
    24481 acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat tggcttccag
    24541 cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc ctttgcaacc
    24601 taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg acgcttccag
    24661 acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat aatcatagaa
    24721 ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta tcgcaaccgt
    24781 gaactccggc ccacttagcc atttttcaat caatacttcc tcatcgtgct gaaatgccaa
    24841 tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca cactggaacc
    24901 ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg ctaactgctt
    24961 atcactcagg cctttttcaa actctgcgcg ggttaacgct acccacggag cgaccggtaa
    25021 accagcgccc tgccaaagca atttgctgcg tagtttatcc attgaaagcg cagatgccat
    25081 cactccgctg ccggtataag gcaagcccat cagctcgagc atgccctgca gcgtgccatc
    25141 ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aagcccatcg acttcagttg
    25201 cgtcacgtcg acttctttcg ggtcgacagg atacgcgtca ataccgcctt cacgcagccc
    25261 ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggagg tcccacccaa
    25321 caggaccgcg attttatcag tcatgttgtg cttcctccgg agtttgcggc ttcagtttga
    25381 tttcagctaa agaacgggca atttttccaa tattaccagc cccctgaacg agaatcaggt
    25441 cgttaccggt taataccggt gccagcatct cggctacctg cgccgggtca ggcaccagaa
    25501 tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg tccgctcccg
    25561 gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca acctgcgtca
    25621 gcacattggc gaaatcatca tacaggtcgc gtgtacgggt aaaacggtgc ggctgaaaca
    25681 gcattaccag gtttttatcc ggccagcctg cacgcgccgc tttaatagtg gcgtccactt
    25741 ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta ccattcactg
    25801 gctccagcgg gaattcaccg aggaaatcaa aacgacgacc agtcccctgg aagctctcca
    25861 gcgcacgcaa aatagcctcg tcgtcaatgc cctcttccgt agcaaccgca accgcagctg
    25921 cggcgttcag cgcgttatga cgacctggcg catttagggt gacgcgcatc ggctctttgt
    25981 cctggcgcag cagcgtaaag tgcccttgcg ggccaatctg ctgataatct tctacacgca
    26041 cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc ggtaacaatt
    26101 cgcggatcac cggatcatca acacacatca ctgcacgacc gtaaaacggc aggttgtgca
    26161 gaaaattaat aaaggtctgt tttaaattct caaagtcgcc ctggtaggta tccatgtggt
    26221 cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac gatgcatcac
    26281 tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga acccccgccg
    26341 cttttaccag cccaccgtta acgaaggttg ggtcgagccc cgcttctgcg tagatgctgg
    26401 aaaccatcgc agtggttgtc gttttgccgt gcgttccggc aatggcgatg ccatgacgaa
    26461 aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc gcttcatgag
    26521 ctgcgacaat ttccgggtta tcggcagaaa tcgcgctgga aacaacgacc acgctggcat
    26581 cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc cccaagattc attaactgtt
    26641 gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca ttggccagaa
    26701 cttcggcaat accgcccata ccggcaccac cgatgccgac aaaatgtatg tgccgaacgc
    26761 gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc attctttacg
    26821 ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg cccgggcagc
    26881 ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcgg cgcgggcgcg
    26941 ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg cgacagcatc
    27001 cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca gcggtagcgc
    27061 attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg gtagtcctgc
    27121 cgcggcgatt tcactcaccg ttaacgcacc ggagcggcaa acgacgacat ccgcccacgc
    27181 atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt gccccgcttc
    27241 ggcatacgcc tgttcaacgg attgttgcga acctttgccg ctctgatgcc agatagtgac
    27301 tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc gtgcgccctg
    27361 ggaccccccc actaccaaca cacgaaccgg accttcacgt ccagccaaac gttgctgcgg
    27421 caacggcagc gccagcacat cggtacgcac cgggttaccc accacttccg cattagggaa
    27481 agcgcctgga aacgcctgca tcactttggt ggcaatctta gccagccatt tattggttaa
    27541 gcccgcaata ccgttttgtt catgaagcac aaccggaatg cctaacgacc acgcggccag
    27601 accacctgga cctgatacgt agccgcccat accgagcacc acgtcaggtt tgtacgcttt
    27661 cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggggcag ctatcagtgc
    27721 ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga tgccatgttt
    27781 tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt gccaaccctg
    27841 agccattaga tggtgcgcaa ccgccagccc cgggaataca tgtccaccgg ttccgcctgc
    27901 catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa cgcctgcgct
    27961 ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt cgacataatc
    28021 agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt cggtaacatc
    28081 cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc aatggaacag
    28141 gcgagaaaac cggaaaaacg atggtcaatt tctaatgctt tacggccaat cgacatcgca
    28201 cgaaaagcga cgaagaatac cattaaaagt gctagtacca caccgacata acctaattct
    28261 tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc cagtttttgt
    28321 accgagttac ctaaaccttg cccccaaagt tcgccgcgac caaacgccat cagcgattgc
    28381 gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc ggtaacacgg
    28441 cggatacggt acggttcggc gaggatcagc aacacaaccg ctgaaatgcc cataccgata
    28501 atggcaatga actgccacaa tttcgctccc gccaggaata acatcgccag cgtagtcaca
    28561 aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc caacaccaga
    28621 atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc gcctttacgc
    28681 accagatagt tggcgatata gcaaaacaac gacagttttg tcagctccgc aggctggata
    28741 cgcagcaagc cgagatcgat ccaacgcgat gcccctttaa cagagctacc cactaccaga
    28801 acgatcatca acaggatgat tgatccgagt agcatcgtag cgctgtaacg ttgccagaac
    28861 tccatcggca gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag atagacgcca
    28921 tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg cattgacgcc
    28981 gaggtcacca tgataaagcc aatcgccgcg aggccgaagg tcagccacag taaggtgcga
    29041 tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca gccctttagc
    29101 gccgtggaga tccagaccag aatactgaat cctggcaggc gcggcatttt caggcgaggg
    29161 agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg cctcgttgtt
    29221 caaagttctt gaactgatca aggctggcac aggccgggga gagcagaacc atatcgcccg
    29281 gctgaacacg cggagccagc aagcgcatcg cctgttccat ggtttcggtt tgttctgcca
    29341 cttccgggcg tagcgccgcc agctgcgcgc cgtcccgacc gaaacaatac agacgtacgt
    29401 tatcgccatt caggtaacgc gccagtgggc taaaatctgc cgatttacca tcgccgccca
    29461 gcaacaaatg cagcgtgccg tctacgtgca ggccattcag tgccgcttcc gtactgccga
    29521 cgttggtcgc ttttgaatcg ttaatccagc gcacaccgtt atgctccagc acaacttcaa
    29581 agcgatgcgg cagaccagtg aatgtggtta acgctttcag gctgctggcg cgcggcaaac
    29641 cagctgcgtc tgccagtgcc agtgctgcga gcgcattggt gtagttatgc tgcccggaaa
    29701 gtttcatttc tttcacgttc agcactttct cgcctttcac ccgcagccag gtttcgccct
    29761 gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag cgttcatccg
    29821 caccgcgaat cggcattgtt aaggcatcat cagcattaac cacgcaaatt tttgcgtttt
    29881 cgtaaatgcg cagttttgct gcacgatact gttgtaaacc aaacggatag cgatccatat
    29941 gatcttcagt tacgttcaga atggtcgctg ctaccgcctg taaactggag gtggtttcaa
    30001 gctggaagct cgacagctct agcacgtaca gttcgcactc atcatccagc agcatcaacg
    30061 caggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc gccatttcac
    30121 ccactagagt ggtggccgtg cttttgccgt tagaaccggt gatcgccaca atcggtgctt
    30181 gtgcttcacg acagaacagc tcgatatcgc caacgatttc gattccggca tcagcggcag
    30241 cgcttaagga tggatgcgcc agtgcaatac cgggactggc gacaatcaga tctgccgcca
    30301 tcagccattc atcattcaga ccgcccgtgt ggcgttctac ggcttcgggt aatttatcca
    30361 ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga gcgaggaaaa
    30421 agtccacgca agaaagcccg gtgaggccca ggccgataat gacgacattt ttaccctggt
    30481 aatcagccat gattaacgta ccttcagcgt tgccagacca atcaggacca gcatcagcga
    30541 aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt catagtggtg
    30601 atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc cgacctgcag
    30661 gatgacggaa agtgtttcta ccacgaacac gccccccata atcaccagca ggaattcctg
    30721 acgcagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac cgacatcgcc
    30781 cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagccctg ccccgactat
    30841 cgcggtacag acaatgacca gttccccggc gtgtcgtaga tacggtatgt gtaaatagct
    30901 ggcaaagttc atgttgccgg tcgcccacgc caccagcgca aaaccaccgg agacaaatac
    30961 ggtcggcata attgccagac cgtcgagacc atcagtcagg tttaccgcgt tgcctgtacc
    31021 cacaatgacg aagtaagcca gcagaatgta gaacagcccc agttgcggca tcacatcttt
    31081 aaagaacgga accaccagct gcgttgcggg cgtgtctttg ccggcaaggt acagcgcgaa
    31141 ggcaacaccc agcgcaatga ccgacatcca gaaatacttc caacgagcga tcaacccttt
    31201 ggtgtcttta cgcaccactt tgcgatagtc atcaacaaag ccaatgatgc cgtaacctac
    31261 cagcaccacc agtacgcacc agacgtacgg attggatgga taagcccaca gcagcacgga
    31321 gatcacaatc gctgtcagga tcataatccc gcccatggtc ggcgtaccgc gcttgctgaa
    31381 gtgtgattca ggaccgtcgt tacgcactac ctgaccaaag gaaagttttt gcaaatgagc
    31441 aatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc tgacgatggc
    31501 gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca aatgttcggc
    31561 cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc catggcggca
    31621 ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag tgatttaata
    31681 cgcgcgataa gcgctgtttt atctgcaaaa tgttcgccaa cgccgctggc ggtgctgata
    31741 gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt tgccgcttcg
    31801 cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc gcccaccacc
    31861 agcacgcggt agcccggcat ttcagccagt acctggactg ctgcggtcat tgaaccgaca
    31921 ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat ggggaacaga
    31981 cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag cgttgcgccc
    32041 acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg ccccggcaac
    32101 ggcagcagaa catccacgct accggttggg gtttgcaggg taaattccgt accgtgcgaa
    32161 gtcacatgga tgttggtggc ggtgaaatcg ctgttggcgg cattgggtga gaaacgccac
    32221 actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc ggcattcata
    32281 atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt cgcgacaccc
    32341 gcaagcgagc caaaaccttc cagatgtgcc gctgccaggt tgttgaccag cgccgcttcc
    32401 gggcgagtca gactcacagt ccaggcaatt tcgccctgat ggttcgcgcc aagttcaatg
    32461 actgcgtaat cgtattccga cgttaagcgc agcagcgtca tcggtacgcc gatgtcgttg
    32521 ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc cgccgtcatc
    32581 tctttaacgg aggttttgcc ggaggaaccc gtcagagcaa ccacgcgcgc cggaacttgc
    32641 tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac gattaactgc
    32701 ggcagatcga tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc agctttcgcc
    32761 tggtcggcaa aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa caggcagccc
    32821 ggcgtcagtt ttcgcgtgtc agtggttaca gcatcaaggg taatatctgc accttgcagt
    32881 tcaccgttga gaatatcggt aagttggcta agggttacgc taatcatgca atcaccccca
    32941 gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca acaatctggt
    33001 aatcctcatg acctttgccc gcgaccagta ccacatcatt ctctttagcc tgcataacgg
    33061 cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca tctaacattc
    33121 ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta tcgtccgtca
    33181 ccaccgccac gtcagcaaac tcttcagcaa ttgcgcccat cagtggacgt ttgcctttat
    33241 cacgatcgcc accacagcca aagacgcacc acagcttgcc tgcacagtgc aggcgcgccg
    33301 cctgtaaggc tttttccagt gcatctggcg tatgcgcgta atccaccacc accgtcggtt
    33361 tgcctggtgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc gcggcggttt
    33421 tcagcaaatc agccagcgga tagccgagtg ccagcagcgt cgccagcgcc agcagcaagt
    33481 tgctgacgtt aaaagcgccc atcagatggc tttcaatttc gccatcgccc cagcttgagc
    33541 taaagcgaat cgtcgcaccg ctgtcgtgat agttcacgtc ggtcgctttc aaccagcgtc
    33601 cgtgacagtt cggattgata tgatcttcca ttgacaccgc aactgcgtct ggcagttttg
    33661 ccagccagcg gcgacccact tcatcgtcgg cgttaacaat cgcctgaccg caatgatgtg
    33721 cagaataaag cagccatttc gcggcttcgt agtgttccat atcaccatga taatcaaggt
    33781 gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc acacggtgct
    33841 gtaccagccc gtgggaggaa acttccattg cgcaaaaagt cgcgccctga tccaccagcc
    33901 ccgccagctc atgctgaaca tcgactgccg aaccggttgt attttctgtc gggatcactt
    33961 tccccagcag gccgttacca acggtgccca ttaccgcgct ggtttcgcca agcagttggc
    34021 tccactgcgc cagcagctgg gtcgtcgtgg ttttgccgtt ggtgcccgtt acgcctacaa
    34081 gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat aaacgctcgt
    34141 tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca ccgtcggtcg
    34201 cctcatcttt cgcctctgca ataatggcag ccacaccttg cgctatcgcc tgcgggatat
    34261 atcgacgccc gtccgcctga tgacctacta cagctacaaa gagatcgccc gccgcagcca
    34321 cacggctgtc gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc acccacggag
    34381 caagaaggtc gcgcaaatta cgatctgcca cctgtcccct cgccttgatt aatcacaaat
    34441 tcatttttat cgcccgttgt cagcgcatcc ggctcgatgt tcatggtacg caatacgccg
    34501 cccatgatgg caccaaagac cggcgcggaa acggcgccgc cgtagtattt acccgcctgc
    34561 ggatcgttga taacaacaac cagcgcgaag cgcggctgac tcgcaggcgc aacgcctgcg
    34621 gtataagcaa tatatttatt gatgtagcga ccgtccggcc cgaccttttt cgcggtaccg
    34681 gttttaatgg cgatacgata gcccttaatc gccgccttca cgccgccacc acccggtagc
    34741 gccacgcttt ccatcatatg caccacggtg cgaacaatgg attccgggaa gactcgttca
    34801 ccgggaaccg gggggtcaac tttggtaatc gacagtgggc gataaatgcc gtagctgccg
    34861 atagttgcgt agactcgcgc taactgtaac ggtgttacca ttagcccgta gccgaaagag
    34921 aaggtgaccc tctctatgtc agaccaccgt tgtttttgag gatataagcc actgcgttct
    34981 ccgaccaacc ccaaattggt cgcttttccc agtccaaaac gtgagtaagt atctactaac
    35041 gctgaggacg gcatcgctaa cgccagcttg gaaacaccga cgttactcga cttctgtaat
    35101 accccggtca gggttaattc gctgtagcgt gccacgtctt tgatttcgtg gccgttaatt
    35161 cgataaggaa cggtattgag taccgagttt tcccgcacta cgccacgttg caacgcggtc
    35221 atcaccacca tcggtttaac cgttgaaccc ggttcaaaca cgtcggtgat ggtacggtta
    35281 cgcatcgcct ctttcggcgt gccgctcaga ttgttagggt tgtatgacgg gctgttagcc
    35341 atcgccagca cttcaccggt gttgacatcc accagcacgg cgctaccaga ttcagctttg
    35401 ttaaaggcca ccgcgttgtt cagttcgcga taaaccagcg cctgcaggcg ttcatcaata
    35461 ctcagcgcca ggttgtgcgc cgcctgactg tcggtggaag agatatcttc aattacgcga
    35521 ccatagcggt ctttacgcac aatgcgctca cccggctgcc cggtaagcca tttatcgaaa
    35581 ctcttctcga cgccctcaat cccctgacta tcgacgttag taaagccgat gaggtgagca
    35641 gtcacttcgc cggatggata ataacggcga gattcttcac gcagatgaat ccccggcagt
    35701 ttcagttttt tgatgtagtc cgccatgtca gggtttacct gacgcgccag ataaataaag
    35761 cgccctttcg ggttggcgtt aatgcgggct gaaagctgat ccagcggaat attgagcgcg
    35821 ttagccagcg ccttccagcg gtcaccgacg ctgataccgc cagcgtcatg cacttctttc
    35881 gggtcagccc aaatcgcttt taccggcacg ctcaccgcta acgggcgacc agaacggtca
    35941 gtaatcatgc cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat gtcgccctct
    36001 ttcaccagca tatccgggga gataacttgt aaccacgcta cgcgtccgag cagaaaagcc
    36061 agcgccagga gaatacagcc gcataacaac gcaaaacgcc aactgataaa gttggcatgt
    36121 tcttcctgac gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg cgtttatcct
    36181 tatttttgca ctacgatatt ttcttgtgac ggatcaacat gctgcatttg cagcttttcc
    36241 gtggcgatcc tttccacccg gctatggtcg ccgagcgcat tctcttcaag gatcaggttg
    36301 cgccattcaa tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg agcggtcagt
    36361 aaacgggtat ggtgcgccgt ggttaccacg gtcaccgccg tcaaaataat gcaaatgaac
    36421 aggcagagtg gcagcttccc aaatcgcaaa agatcgtcac cgataacacc aggcaatgca
    36481 tggcgctcgt ggcttcccat cgatccttta actttgctta gagcttctgt cactctgctg
    36541 atcatgcgtt cgtcctctct gcaatacgca gaactgaact acgggcacga gggttctcag
    36601 ccacctcttc ttcgcccggc attaacttgc ctagtgctcg cagctgacgg ccacccagtt
    36661 ttttgagctg ctcttcagtc atcggtaacc ctgccggaac ttgcggaccg cggctgtttt
    36721 cacgcataaa acgtttcaca atacggtctt ccagcgagtg gaagctgatg atcgaaagcc
    36781 gcccacccgg ggccagcacg ttgagcgagc tttttagcgc ctgctctatc tcctccagtt
    36841 cactgtttac ccaaatgcgc accgcctgga aggtccgagt cgcgggatgt ttaaacttat
    36901 ctttcaccgg cgttgcagca gccacgactt ccgccagttc tttggtgcgg gtcatcggct
    36961 gttcgcggtt tcgctcgaca atggcgcggg caatgcgttt agcaaaacgc tcttcaccgt
    37021 aggttttcaa tacccaggcg atatcggctt cttctgcggt ttgtagccat tcagcggctg
    37081 actgcccacg ggttgggtcc atacgcatgt ccagcggacc atcgcgcata aaggaaaagc
    37141 cgcgttcagc atcatcaagt tgcggtgaag agacgccaag atcgaggaga atgccgtcga
    37201 tcttgccgat aagatcgcgc tcggcaacgt attcgcccag cgcggagaaa ggtccgtgga
    37261 tgatggagaa gcgcggatca tcaatagtct tcgcaacggc gatagcctgc gggtcgcgat
    37321 cgatcgccag caaacgcccc tcttcgccaa gctgcgagag gatcaaacgt gagtgaccac
    37381 cgcgaccaaa agtcccatca atgtagatgc catcaggacg gatattgagg ccattaacgg
    37441 cttcatccag cagcaccgta gtatgtttat agttttccat cattttatag agacaagtcc
    37501 tgcagtcgct ccgataagtc tccggtagcc aactgctctg cgtcgatatc ttccttgacc
    37561 tgttgatgcc aggttgtttc atcccacagc tcaaacttgt tgaactgtcc aaccagcatc
    37621 acttcttttg tcagcccggc atgttgccgc agtactggcg cgattaacaa tcgacctgcg
    37681 ccatccatct gacattcgct ggcatgacct aacagtaggc gctgcacacg gcgctcaacc
    37741 gggttcatgc tcgacagacg cgataatttt tgctcgataa tttcccattc aggcaggggg
    37801 taaagcagca ggcacgggtg atgaatgtca atggtgcaaa ccatttgacc ggcagcgttc
    37861 tcaagcagct gttcccgata acgggtaggc actgataagc gccctttgct gtcgagattg
    37921 actaacgttg ctccccggaa catgccagcc tcacccctta ttcccacttt gccccacaat
    37981 ttcccactga aaggagttta cggagcggag gaaaagcttg tcaagccagt cacaaggcat
    38041 accgaggtaa aaaacccgat gtttacggct aatatcagcg ttgtttattt tacgtccaac
    38101 gtgcgaagca aattaacgtt atgaatagct gtaagaaaaa atccctcatc gtcatcaccg
    38161 ttttaaaatg actcactatt ttccacagca aatataaagt gtcagtttgc gacgcgagcg
    38221 gcattttagg acatatcttc cccggttaac agtccctgtt gcgtctgtgt ggcgcttgat
    38281 gccagcgggt ttgcgcaaaa gcaaagaaaa gtgtttgtta aatcggcaat tcatctctgc
    38341 atgtaacaaa ataatgcaaa aacgcgctat cgatatcact taattattgt gaatttcagc
    38401 tggtgaggta attaacgggc aggtttaaaa gcatttatca ataatagcca ggaatagtct
    38461 tatttacttt aaggatattg atgtccattc cgtactcaac gcgccagaga gaaattcacc
    38521 tggcgcgtat ttttgttcgc ggcttagcta cggctgagca cgccgcggcg atagagatta
    38581 cgtttaatgc gcgttaaacc aggttttggc ttacgcggtt cgtccaggct tgccaggaca
    38641 atctccagca cacgctctgc gacatcgcgg tgacgttgag ccactgccag caccgggcac
    38701 tgtaagaagt cgagcagttc gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt
    38761 ttgccgtcac gacgtagcgt gacatccatc accccttgca acaacgcaaa cgacgttgtg
    38821 aacagtgcct gcggcatcgg atgcgtttcc agccattttt cgaataactg ggcagccgcc
    38881 tcccgctcat agctgttggc ataaaggaaa tgcacttcgc gcggatcatc tttccaggca
    38941 gtacggaaac cttgttcacg caggaagctg acagaaagct ccggcagcgc gccaagataa
    39001 agtaccgtct cggcgggaaa cttacgtaac tcttccgcca gcatttcggc atcatcctga
    39061 tcggcaccaa ccacgctggt gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc
    39121 gggaacgggt cgttagccca gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa
    39181 acaataatgg catcaacctg acgctgcaaa agatgctcga tacaacgcat ttcgttgtcg
    39241 ggctgatctt ccgagcaggc aatcagcagt tgataacccc tttgccgcgc ctggcgttca
    39301 agatagttag cgatgcgggt atagctggtg ttctccagat cggggatcac aagaccaata
    39361 gaacgtgtgc gtccagcacg aagcccagct gccacggcgt tcgggtggta attgtgctca
    39421 cgcaccacag ccatgacttt ttcaacggtt ttgtcgctca cacggtattg cttcgctttg
    39481 ccgttaataa catagcttgc agtggtccgc gacactcccg ccagccgagc gatttcatcc
    39541 agtttcacaa ttgccccttg cgtaaaatgt aaaaaccata gccattgtac tggtatgggt
    39601 taaattcatt aacatctaag cgcagaaaat taactgcggc aactgctttt attccaggtt
    39661 cccactgatt tcgcaaaaaa agcccaacgt gacatgttgg gctgcaaatt gcgcattgag
    39721 atcattatca acgcattatt ttatcgccgc gcgaaagtcc taccacacca gagcgagcaa
    39781 cttccacaat tttcgccaca tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg
    39841 tgcctgctaa ttgaacggta taaagcgagg gtgtgacatc aataatttgc ccacggaata
    39901 tttccgtatt acgtttcact tcgtcacgcc cgtagccgct ggcctgaatt ttcaccagca
    39961 tgatttcccg ctcaacatgc gcgccctgcc ctaactcgct cacgcgtaat acatcgacca
    40021 gcttgtgtaa ttgcttttcg atctgctcaa gcactttttc atcgcccacg gtctggatgg
    40081 tcatacgcga taatgtcgga tcgtcggttg gcgcaacggt caggctttca atgttgtagc
    40141 cacgctggga aaaaaggcca atcacgcggg ataacgcgcc tgattcattt tcgagtaaga
    40201 ctgataatat ccggcgcata atcaggttct ctccgttttg cttaaccaca tttcatccat
    40261 tccgcccccg cgaatctgca tcgggtagac gtgctcactg ccatcgacgg taacatcaac
    40321 aaacaccagg cgattattgc gcacctgttc cagcgcctcg ctaagtttgc tttccagttc
    40381 ttgcggatga gaaatctgga tcccgacgtg accataggct tccgccagac gaacgaaatc
    40441 gggtagcgat tgcatataag attgtgaatg acggccggaa tagatcatgt cctgccactg
    40501 tttcaccatc cccagatagc ggttattgag attcaccacc agtaccggca actcgtattg
    40561 caacgctgta gacagttcct ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac
    40621 cacggtttct tctggcaacg ccattttgac gcccagtgcc gcaggtaaac caaaccccat
    40681 cgtgccgagg cccccagagt tgatccagcg acgcggtttg tcgaatggat aataaagcgc
    40741 agcaaacatc tggtgctgcc cgacatcgga cgtcacgtaa gcgtctccct tcgtcaaccg
    40801 ccaaagagtc tcgatcaccg cctgcggttt aatcttttca ctgtaagagt catatttcag
    40861 gcactgacga gcgcgccact gttcaatctg ctgccaccag tcgcggatct catccagcgg
    40921 ttgatggacg gattcttgcg acaggagttc aagcatttgt tcgaggacct ggcgagcatc
    40981 ccccacaatc gggatatcag cagtcacggt tttagaaatg gaagtaggat caatatcgat
    41041 atgcagaacc gtggcatttg ggcagtactt cgccagattg ttcgtcgttc ggtcatcaaa
    41101 acgtaccccg acggcaaaaa tcacatccgc gttatgcatc gtcatattag cttcgtaggt
    41161 gccgtgcatt cccagcatgc caagtgcctg acgatgcgtt gccggaaacg cccccagccc
    41221 cattaatgag gaaacaacgg gcagattcaa cgcctccacc gtttctttta gctgctgatg
    41281 acagcccgcc gtgattgccc caccgcctac atagacaacc ggtttttttg ccgccaccag
    41341 cgtttgcaga gcacgcttaa tttgcccttt atgtccggta gtagtgggat tgtaagaacg
    41401 catactgacc gactccggcc agacgtaggg taatttgttc gccggattaa gaatatcttt
    41461 gggtaaatca acgaccacgg gcccagggcg accacttgcc gccagccaga aagccttttt
    41521 cagcacctgc ggaatgtctt ccgtttgctt aaccagaaaa ctgtgtttaa ccaccgggcg
    41581 agaaatcccc accatgtcgc actcctgaaa ggcatcgtag cctatcaacg aggtcgctac
    41641 ctgcccggaa aggacaacta agggaatgga atccatataa gcggtggcaa tgccagtaat
    41701 cgcattggtc gcccctggac ccgacgttac cagcacgacg ccgacttccc cggtcgcgcg
    41761 cgccagacca tcggccatat gcaccgccgc ctgctcatga cgaactaaaa catgatcgat
    41821 accacccacg gtatgcaatg catcgtaaat atcaaggacc gcgcctccgg gataaccgaa
    41881 tacttgttta acgccctgat cgataagcga tcggacgacc atctcggctc cagacaacat
    41941 ctccatggcc tgcctcactg tttgacggaa aaaatgtgta aaacacatta accgtccgat
    42001 cttgcttaag caattggcag cctaagacat aaacacggag taataagaat aaacaggagg
    42061 aaaggtgaaa agaaacgata aaatcctcca ttacagagga ttaaattgat aataaatcgg
    42121 ctgaatccca caacttacag caatttctct atttttttaa ttaatcctaa aatagcgaga
    42181 tcacacattc agaataaaat tctacattca ataaaaaatg cagaataaac cagacattaa
    42241 tgtctgacct attctgcatt cagttagcgt ttgcaaattg agactaattg ctcttccatc
    42301 cactgatggc ctttatcgcg cccagcagct tcatgccagg agagataaca ggttctgcta
    42361 ttttgtttta acggtagcgg taatacctgt aactctaagg attcagcgaa ctcttcagcc
    42421 agccaacgcg gcgctatagc gaccagatgc gtttgcgaca ccacgctaag tacgctcatc
    42481 attgccatgc cctgatacgc gatactggct tgcttatcta ccgtgtcata ccacggttga
    42541 ctaaatgacg cgaaacgatc gagcgaaacc gccgcatgtt gttcgttata aacatcatgt
    42601 ttcagtaacg ggcctttaat tgttggatga tttttgctgg ctaccagcac catttcatct
    42661 ttaaataatg gcacactggt aaattcagga cgatggaagt cttcataact aatcacaaac
    42721 tccgtttcct gataacgcag ctgatgttca gtgttctgat ttaatgaaga cttgaacata
    42781 acatgtatat ttggcgcaat ctgctcaatg tgattataaa tctgcgaggt cagaatgctg
    42841 tctaacgggc tgcagacaca aagatgaaat atacgttcac tactggcagg ctcaaagcct
    42901 gaacctggta attcattttg tactaattgc aatgcctgac gaactgaacc aaaaagttga
    42961 aatgcgcgag cagtcggttg aataccacgg ccataacgaa caaaaagctc gtcattaaac
    43021 atcaccttca ggcgtgcaac agcgttactg accgcaggtt gcgacattcc cagaacatga
    43081 gcggcacggg tgatgttttg ctcctgcatc acggcatcga aaacggttaa taagttgaga
    43141 tcgaccatgc gtagctgtgg cttgcttaac tccgccgtct ctggatgatc tgtttgtacc
    43201 tctggcatac ttaactccac tgtcacactt aactcccttt cccttattgg aatgcagaga
    43261 aataatcctc gagaatatga tttatcatgc atataaaata agaaaaagga aaatgagtaa
    43321 aattaggaaa acataaagat acgggttctg ataaaaacag aatccactgc cgagcaaagc
    43381 gtcatttacc acagcataat aatccataat gtttttgttg cgaaaacaat ctaatcataa
    43441 ctaccacgaa tactcaatca tccacaaaat ggattaaatg tgctttttta ataattaatt
    43501 tatatgttta atgcattaat tcttaacatt aatcgatcaa taatattcac caaatgaata
    43561 tcaaaaaatt cgcaaaacat ataattcaat acaaatcatc aggataggtt ttgtaacgcg
    43621 tgcattttat ccccctttcc ctcgtcgatt agacgcaaaa atttattctg aaatatgtca
    43681 accgatgaaa agcggcggtg gttaagcaga aattaatatc gcttgtttta accaccgcag
    43741 cacaattagc taatttgacg gatgcagaac tcacgctggc tggacgtttt tgttgcatca
    43801 gggttgacat ccgttttggt atccagtaac tctaaaagca tatcgcattc atctggagct
    43861 gatttaatga ctcacatcgt tcgctttatc ggtctactac tactaaacgc atcttctttg
    43921 cgcggtagac tggtgagcgg cattcagaat taagtcagca c
//
LOCUS       DAPOZO010000035        43563 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPOZO010000035 DAPOZO010000000
VERSION     DAPOZO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.035"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            8..940
                     /locus_tag="ROI21_002045"
     CDS             8..940
                     /locus_tag="ROI21_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDX5110965.1"
                     /translation="MIIFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLL
                     IDPSRDGQIVDANLAALNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKP
                     LNFVHKLADGSTRHVQTYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTG
                     LLNRRQFYHITEPGQMQHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCTLARTL
                     ESCARKGDLVFRWGGEEFVLLLPRTPLDTALSLAETIRVSVAKVGISGLPRFTVSIGV
                     AHHEGNESIDELFKRVDDALYRAKNDGRNRVLAA"
     gene            complement(961..1524)
                     /gene="smrA"
                     /locus_tag="ROI21_002046"
     CDS             complement(961..1524)
                     /gene="smrA"
                     /locus_tag="ROI21_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDX5110966.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(1719..3233)
                     /locus_tag="ROI21_002047"
     CDS             complement(1719..3233)
                     /locus_tag="ROI21_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDX5110967.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPMFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGTPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
     gene            complement(3230..4258)
                     /locus_tag="ROI21_002048"
     CDS             complement(3230..4258)
                     /locus_tag="ROI21_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDX5110968.1"
                     /translation="MIISKKQLIGVVAIGIILAGVAFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQVIDNARFDYQ
                     QVTAKERKAANDFMVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            4355..5170
                     /locus_tag="ROI21_002049"
     CDS             4355..5170
                     /locus_tag="ROI21_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX5110969.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            complement(5343..6251)
                     /locus_tag="ROI21_002050"
     CDS             complement(5343..6251)
                     /locus_tag="ROI21_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5110970.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKATYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            6427..7737
                     /gene="abgA"
                     /locus_tag="ROI21_002051"
     CDS             6427..7737
                     /gene="abgA"
                     /locus_tag="ROI21_002051"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDX5110971.1"
                     /translation="MESLNQFVNSFAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALEQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEERDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFERAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVPGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YMVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            7737..9182
                     /gene="abgB"
                     /locus_tag="ROI21_002052"
     CDS             7737..9182
                     /gene="abgB"
                     /locus_tag="ROI21_002052"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDX5110972.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQCGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYRALSHFGTPEWNCEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            9219..10745
                     /gene="abgT"
                     /locus_tag="ROI21_002053"
     CDS             9219..10745
                     /gene="abgT"
                     /locus_tag="ROI21_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HDX5110973.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            10756..11271
                     /gene="ogt"
                     /locus_tag="ROI21_002054"
     CDS             10756..11271
                     /gene="ogt"
                     /locus_tag="ROI21_002054"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDX5110974.1"
                     /translation="MLRLLEEKIVTPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            11466..12218
                     /gene="fnr"
                     /locus_tag="ROI21_002055"
     CDS             11466..12218
                     /gene="fnr"
                     /locus_tag="ROI21_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDX5110975.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            12370..13320
                     /gene="uspE"
                     /locus_tag="ROI21_002056"
     CDS             12370..13320
                     /gene="uspE"
                     /locus_tag="ROI21_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDX5110976.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(13370..13627)
                     /locus_tag="ROI21_002057"
     CDS             complement(13370..13627)
                     /locus_tag="ROI21_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDX5110977.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            13871..14902
                     /gene="ynaI"
                     /locus_tag="ROI21_002058"
     CDS             13871..14902
                     /gene="ynaI"
                     /locus_tag="ROI21_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDX5110978.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVSEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            complement(14953..16566)
                     /gene="mppA"
                     /locus_tag="ROI21_002059"
     CDS             complement(14953..16566)
                     /gene="mppA"
                     /locus_tag="ROI21_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDX5110979.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGKLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(16903..17802)
                     /gene="pgrR"
                     /locus_tag="ROI21_002060"
     CDS             complement(16903..17802)
                     /gene="pgrR"
                     /locus_tag="ROI21_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDX5110980.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            17940..18860
                     /locus_tag="ROI21_002061"
     CDS             17940..18860
                     /locus_tag="ROI21_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX5110981.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNSRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            18931..19569
                     /locus_tag="ROI21_002062"
     CDS             18931..19569
                     /locus_tag="ROI21_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX5110982.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVICEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            19860..20588
                     /gene="mpaA"
                     /locus_tag="ROI21_002063"
     CDS             19860..20588
                     /gene="mpaA"
                     /locus_tag="ROI21_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDX5110983.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPL"
     gene            complement(20563..21528)
                     /gene="ycjG"
                     /locus_tag="ROI21_002064"
     CDS             complement(20563..21528)
                     /gene="ycjG"
                     /locus_tag="ROI21_002064"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDX5110984.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQESLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            21647..22153
                     /gene="tpx"
                     /locus_tag="ROI21_002065"
     CDS             21647..22153
                     /gene="tpx"
                     /locus_tag="ROI21_002065"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDX5110985.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(22197..23738)
                     /gene="tyrR"
                     /locus_tag="ROI21_002066"
     CDS             complement(22197..23738)
                     /gene="tyrR"
                     /locus_tag="ROI21_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDX5110986.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRSIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(23886..24947)
                     /locus_tag="ROI21_002067"
     CDS             complement(23886..24947)
                     /locus_tag="ROI21_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDX5110987.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(24944..26341)
                     /locus_tag="ROI21_002068"
     CDS             complement(24944..26341)
                     /locus_tag="ROI21_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDX5110988.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTEYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            26496..27494
                     /locus_tag="ROI21_002069"
     CDS             26496..27494
                     /locus_tag="ROI21_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDX5110989.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(27605..28510)
                     /gene="ompG"
                     /locus_tag="ROI21_002070"
     CDS             complement(27605..28510)
                     /gene="ompG"
                     /locus_tag="ROI21_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDX5110990.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(28555..29637)
                     /locus_tag="ROI21_002071"
     CDS             complement(28555..29637)
                     /locus_tag="ROI21_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5110991.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(29651..30310)
                     /gene="pgmB"
                     /locus_tag="ROI21_002072"
     CDS             complement(29651..30310)
                     /gene="pgmB"
                     /locus_tag="ROI21_002072"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDX5110992.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     TDLCAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(30307..32574)
                     /gene="ycjT"
                     /locus_tag="ROI21_002073"
     CDS             complement(30307..32574)
                     /gene="ycjT"
                     /locus_tag="ROI21_002073"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDX5110993.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFALSRETWQRELDFASGELR
                     RNVVWCTSNGARYTIVSRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETLTLQKLVWFDWRDDRHVALDEWGSASLRQLEMCVQQSYDQLLAASTENWR
                     QWWQKRRITVKSNDEHNQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVVQYWQTTGDESFIAHEGMALLLETAK
                     FWISRTVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIYDSSLSKAIHDIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELNLNPA
                     LPVQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(32571..33626)
                     /locus_tag="ROI21_002074"
     CDS             complement(32571..33626)
                     /locus_tag="ROI21_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDX5110994.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(33636..34424)
                     /locus_tag="ROI21_002075"
     CDS             complement(33636..34424)
                     /locus_tag="ROI21_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDX5110995.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMASPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(34443..35495)
                     /locus_tag="ROI21_002076"
     CDS             complement(34443..35495)
                     /locus_tag="ROI21_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDX5110996.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPELSIKMGVTF"
     gene            complement(35526..36368)
                     /locus_tag="ROI21_002077"
     CDS             complement(35526..36368)
                     /locus_tag="ROI21_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDX5110997.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(36355..37236)
                     /locus_tag="ROI21_002078"
     CDS             complement(36355..37236)
                     /locus_tag="ROI21_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDX5110998.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(37257..38549)
                     /locus_tag="ROI21_002079"
     CDS             complement(37257..38549)
                     /locus_tag="ROI21_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX5110999.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENSGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPLAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(38564..40243)
                     /gene="ycjM"
                     /locus_tag="ROI21_002080"
     CDS             complement(38564..40243)
                     /gene="ycjM"
                     /locus_tag="ROI21_002080"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDX5111000.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HNEEITRELNDEDTLRHAVYHQLSRLITLRCSHNEFHPDNDFTIDTVNSSVMRIQRNN
                     ADGNCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(40457..40771)
                     /gene="pspE"
                     /locus_tag="ROI21_002081"
     CDS             complement(40457..40771)
                     /gene="pspE"
                     /locus_tag="ROI21_002081"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDX5111001.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(40846..41067)
                     /gene="pspD"
                     /locus_tag="ROI21_002082"
     CDS             complement(40846..41067)
                     /gene="pspD"
                     /locus_tag="ROI21_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDX5111002.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(41076..41435)
                     /gene="pspC"
                     /locus_tag="ROI21_002083"
     CDS             complement(41076..41435)
                     /gene="pspC"
                     /locus_tag="ROI21_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDX5111003.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(41435..41659)
                     /gene="pspB"
                     /locus_tag="ROI21_002084"
     CDS             complement(41435..41659)
                     /gene="pspB"
                     /locus_tag="ROI21_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDX5111004.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(41713..42381)
                     /gene="pspA"
                     /locus_tag="ROI21_002085"
     CDS             complement(41713..42381)
                     /gene="pspA"
                     /locus_tag="ROI21_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDX5111005.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            42548..43525
                     /gene="pspF"
                     /locus_tag="ROI21_002086"
     CDS             42548..43525
                     /gene="pspF"
                     /locus_tag="ROI21_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDX5111006.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
BASE COUNT        10953 a        11283 c        10369 g        10958 t
ORIGIN      
        1 agaaccggtg attattttcg aaggtatcga agcaccggca acgctgggtt taaaagccag
       61 tcgctcggca aattatcagc gcaaaaaaca aggtttttat gcgcgctttt ttctgactaa
      121 ctctgcacca atgttgttga ttgacccctc acgagatggc caaatcgtcg atgctaacct
      181 cgccgcgctc aatttctatg gctataacca tgaaacgatg tgccagaaac atacctggga
      241 aataaatatg ctcgggcgac gcgtcatgcc tatcatgcat gaaatttcgc atttacccgg
      301 tggccataag cctttgaatt ttgttcataa actggcggat ggttcgactc gtcatgtgca
      361 gacctatgcc ggtccgattg aaatttatgg cgacaagctc atgttatgta ttgtgcatga
      421 tattactgag caaaaacggc tggaggagca gctggaacat gctgcccacc atgacgcgat
      481 gaccggatta ctgaatcggc gacagtttta tcacattacc gagccaggcc aaatgcagca
      541 cctcgccatc gctcaggatt acagcttatt gctcatcgac accgatcgtt ttaaacacat
      601 taacgatctc tatgggcatt ctaaaggtga tgaggtgtta tgcaccctcg cccgcactct
      661 cgaaagttgc gctcgcaaag gcgatttggt gtttcgttgg ggaggcgaag agtttgtctt
      721 attgctacca agaaccccac tggataccgc gctttcgctg gctgaaacta tccgcgtaag
      781 cgtggcaaaa gtgggtattt cgggcttacc acgctttacc gtcagcattg gtgtggcgca
      841 tcacgaagga aatgaaagca tcgatgaact gtttaaacgc gttgatgatg ctttgtatcg
      901 ggcgaaaaat gatggacgca accgcgtgct ggcggcataa gccgcggatg cgtctcgaga
      961 tcaacgactg cgcttagcgt ggcgctccca gttttcttgc tttgcctgtg ccgttttacg
     1021 tagtgcgacg taacacgccc cgctgccgcc atgatgcggt agcgcggtac aatatgcctg
     1081 gacatcatca aattcggtca accagcgcgc cacatagctg cggacaatat tggcatgcga
     1141 tttatcttcc cgccctttac catgaataat cagcacatta cgcaaaccat ccgccagggc
     1201 ttgttgaatg aaactgaaca ccattttgcg gcactcttcc accggctggc gcaaaagatt
     1261 caggctcgcc tgttgcggat atttaccact gcgcagctta tccagcaccc catgttgcaa
     1321 cccttcccgc cgaaactcca acggctgact tagcgggatg atgtcgagaa atccggtggt
     1381 gaggaaatta tcaagttgca gcgtgtcgat acgctgcggc gcacgttggt tacgcgttgg
     1441 atgccagtgg acatctgtag cacgtttcag cggctggaca tcttccatgg cgtcaagaaa
     1501 cagcgatttg tcgtcaaggt tcatgtaacc tcctatgcag cttcacacgt cgtgattcag
     1561 acgggttaat tgttcatact tactgcatcg ttcccttcac agattgaaac aatactctac
     1621 ttcatacctg tgagtatctg cgcaggcggg gcattttata ctagcctgtt agcagtatcg
     1681 aacagtttag cccgagttta attcagcaca ggctccgatc aggatgattt attcatagca
     1741 ggaaccaaca aaaatgcgat aaataacaga ccactgatta gcagaaaggt atccgaaaat
     1801 gccatagttt gcgcctccct cagcatcaat tgactcaata atttggttga agctagtatt
     1861 tccgatgtct ggtcacttcc actgcgatta aaaaacaaag cacttctgga aatgaagtca
     1921 ttcaccgtat ggggaacaga aaccattttt tcacccattc tcgaaaaatg aaaattagtt
     1981 cgattattga gaatagagcc acacagggca ataccaatcg cgcccccaag attacgtgtt
     2041 aaattaaata caccagaggc taacttcagc cgttcttttg gagttccccc cagcgttaat
     2101 gttacgatgg gggccatcgc aaactgctgc gaaatccctc taattgcctg tggaaacagt
     2161 aactcctgcc atccccattc gtgggttata ggggtaaaaa gatacattga aaacacaaac
     2221 ccacccagcc ccgccatcaa cagccatcgc agatttattt ttttacttaa ccagaagtaa
     2281 aagggaacag aaaataactg gaaaatacct gttgtgcaaa cggcaaaacc tatctcttcg
     2341 gcatttaatc cgcgaacctg ccccaaaaac ataggtatta aatatacagt agagaatatc
     2401 ccaacccctc cagaaaaaga aaaaaaacat cccagcgtga aatttttatc cttaaaagca
     2461 tgaagatcca ttatcggatt ggatattgta agtgtacgcg cagcaaataa aataaaactc
     2521 accaatgcca gaactgatgt cagcaaaata gtattatcat ccagccatcc ccagcgcgct
     2581 ccttcttcaa gggtatattc cagacatccc agtgtcatgg ccaacaggat aatactcgga
     2641 taatcagcaa ctttcagtaa agagagatca ggtttatcaa aattaaccag aaaaggaata
     2701 ctcaaaacta aatatatgcc aggtaatata ttgatataaa atagccatcg ccagtcgagg
     2761 ttatcagtga tccagccccc cagagttggc cctagcgtcg gtgacagtga ggccagcgca
     2821 ctgactaccg cagcagccaa ccccaactct ttaccctggt aataaataaa cgccgtcgtg
     2881 aaaactaaag ggatcatgga tgcgccagcc gctccctgta aagcacgaaa caaaatcatg
     2941 atctgaatat tccatgcaag tccacacgcg atactcatta aagtaaatat tcccgcggat
     3001 agagtaaaaa gccaacgagt ggaaaatacc cgtgacagcc acccagagag cggaataaca
     3061 ataatctcag caattaaata gctggtctga agccaggcca tttcgtcttt acctgcggac
     3121 agaccaccac ctatttcatt tagggatgac gatacaattt gaatatcaag ctgagcaata
     3181 aataatccca tacacatact ggcgaaagca atatgtttac gcatagacat catctctcat
     3241 caacactgac ggtgacggac agccccgggc gaagaagttg ctttaattcc tttgcgtcgt
     3301 caaatgcaat ccgtaccggg acacgctgaa caattttggt gaaattgcca gtggcatttt
     3361 ctatcggaat caagctgaaa ctggctcctg tcgctgggga aaggctttca atgtgaccgt
     3421 gaaacacttc ccctttgaga atatcagcac ggatctcagc cttcattcct ggcttcattc
     3481 ccgctatttg gttttcttta taatttgcat caacccacaa ctcactgact ggtactagtg
     3541 aaactaacga tgtgccgcct tccacccaac tgcctgtatg ggcgctgcgg tttgcgacaa
     3601 tgccatcaat cggcgcacga acaagggtat attccagatc cagtagtgcc tgagtcaaag
     3661 cggcctgaac ttcttcgata cttgcacgta cgttttcttc ctgagcagat aaaactgcca
     3721 gccgttgctt ctccaccatg aaatcgttcg cggctttgcg ctcctttgct gtgacctgct
     3781 ggtagtcaaa ccttgcgtta tcaataactt gttgcgatat tgccgctgat tgtgcgagtt
     3841 tttcataacg ttcagtatct cgcaggcgct tttgctcctc atgttttact gcctgccatg
     3901 tttcactggc actttgaatt atcgactgct gcatcgtaat agtggcttga atgctttcga
     3961 gattggcttt gctacttttt atttttgcct caagcctggc gacatttgca cgataatccc
     4021 gatcatcgag acgcaggata atatcgcctt tttttacgct ctgattatcg cgcacctcta
     4081 tagcagaaat ataaccagaa actttacttg ccaccgtggt gatattacca ccaatgtaag
     4141 catcatccgt cgtctgtata aatcttcctt tgcttaccca ccagataaaa aaggcaacgc
     4201 cagccaaaat aataccgatc gctaccaccc caataagctg ttttttagaa attatcatca
     4261 ctctttacac cgtatatatc cggaaacttg cattcgtcat atttaaaatt taacatggct
     4321 tatttgatga tttctgacaa aatacctact gtaaatgaca atcacactgc aacccaaagc
     4381 atcaccaggg catcacatta tcggactgga ttcggaacat ctgaatggag gcacagttcc
     4441 ctggcataaa catctctatg cccagttgct ttacccggct gaaggagtcg ttcgtgtatg
     4501 ggctggagaa agcgtctggt tagtgcatgc cagcagcgcg ctttggctgc ccccacaaat
     4561 gccgcataag tttgttgcga caggcaacgt acttttaaaa accgtactgg tttcagaagc
     4621 ggaaagtgaa acgctgggaa aagtttgctt catgacgggt atttcacccc tgttaaggga
     4681 attactaatt gcaattaacc aacttccacc gtcgcaaagt accacagata aacaacagtt
     4741 acgtttttca gccctcgaga cactgatact tcaggaaata aaaatggggg tgaaaatgtc
     4801 acttgaactc ccttggccaa atgatgagcg gctacagcag ctttgtgaga atttgctaaa
     4861 caaccaaggg tatctgccaa cactggataa tctagcggat aaaattaacg tcagtagccg
     4921 tacactcatg cgcttatttg ttaaagagac gggattaaca tttcgccact gggttcagca
     4981 aatgcacgtt atttctgcag tgacgcttct tgatgatgga tattcgttga ccaaaattgc
     5041 ccatcgtctg ggctatgcct cagctgaatc gttcggcaat atgtttaagc gtcgaacggg
     5101 ctactcgccc ggtaaattta ctcgtcgatt aacgatgcat gattatgcaa tcacaagaca
     5161 aatgatttaa cttatatttt cgtgaaatct gtcactgaag aaaattggca acggatgggt
     5221 aaaaccgtta taacactgtc accggccacc aggaccccag gccggattta agacgaggat
     5281 gcactgctgt gtgtactgta gagtctggcg gatgtcgaca ggctctattt ttttatgcag
     5341 ttttaacttt gcagatagcc gcattctcgc cgaaattgcg tgattaatga cgcggtcagt
     5401 ggtgtctggc gactatcacg ccgctgaatc aaataatagg tcgctttcgg taaaatttcg
     5461 ctaaccggca acatcaccag cccctgtccg tgcaagggat cgcagcccat ttcttcaggt
     5521 agtatgctga gaaaatcgct ttttgccacc agactgatac aggctgagaa cgtctcgcag
     5581 actacaccga cctgtggcgt ttgcgcctga tcgtcaagca attcactcaa ctgtttgtag
     5641 tagctgccgt gtggcgtcgg cattgtccag ctgtagtcca gtaactgttt gatcgaacgg
     5701 gcaccaatgg cgggatgtcc cgggcggcaa aagatcgcga attgcttttc cagtaatttc
     5761 tcaaaagtaa attcgtggtc gtagggtccc tgataatagg tattgatggt gaaatccagt
     5821 tctccctggc gcaattcatt aatcatcgac accagttgcc cttccataat gcgtactttt
     5881 acctgcggat gctgctgatg aaaacgagat atgacagctg gcatcagact gcgggaaata
     5941 ctggccccca tgccgatatt aatttgccct gccagttgcc cttgtcgttg gcgaatatcc
     6001 tcttgcgctg cgcgcagctc ttcaagaata agactggcgt gctgataaaa actttcaccg
     6061 gcatcagtta acgtcacgcc tttactacgg cgaaaaaaga gttgtgccgc caacccttct
     6121 tccagctcct gaatagattt actcagtgcc ggttgcgaca tattcaacat tcggctcgct
     6181 ccgcgaatgc tgccctgacg agccacttca acaaaagccc gaatttgatg aatttttacc
     6241 tgaaaagcca tgacgccacc gataaccgtt atttatcaga ccaaagaaac tggcatctac
     6301 tttaatgcag atgattgtgt cagggtaatt tatgaacggt taaaactgtg aaaaatcagt
     6361 tagtgataag taaaaactat cgctacgtga accgggtcac actttttact gatgacggga
     6421 aaggttatgg agtctttgaa tcaatttgtt aattcgtttg ccccaaaatt atcgcactgg
     6481 cgacgtgatt ttcaccacta tgcagagtct ggctgggtgg aattccgcac tgccaccctt
     6541 gttgcggaag aattgcacca gctcggttat tcactggcgc tgggccgcga agtagttaat
     6601 gaaagtagcc ggatgggatt acctgatgaa ttcactctac aacgcgaatt cgagcgcgct
     6661 cgtcaacagg gtgcgctaga acaatggatt gcggcttttg aaggcggttt cactggcatc
     6721 gtcgctaccc tggataccgg tcgccccggt ccggtgatgg ctttccgtgt cgatatggac
     6781 gcgctggatc tcagtgaaga acgggatgtc agccatcgcc cctaccgcga cggttttgcg
     6841 tcatgtaacg ccggaatgat gcatgcctgt ggtcatgatg gacataccgc cattggactt
     6901 gggctggcgc atacccttaa acagttcgag tccggactac atggcgtcat caaactgatt
     6961 tttcaacctg cagaagaagg tacgcgtggc gcgcgggcga tggtcgatgc aggtgtagtg
     7021 gatgatgttg attattttac tgccgtgcac attggcactg gcgtacctgc gggcaccgtg
     7081 gtgtgcggca gtgataattt tatggcaacc accaaatttg acgcgcactt caccgggacc
     7141 gccgctcacg caggcgcaaa accagaagac ggtcacaatg ccttgttggc ggcagcacaa
     7201 gcaactcttg cactgcatgc aatcgccccg cacagcgaag gcgcttccag agtaaacgtg
     7261 ggcgttatgc aggcaggaag cggtcgtaat gttgttcccg cctcagcgtt gctgaaagtg
     7321 gaaacacgcg gggccagcga cgtcattaat caatatgttt ttgaacgtgc acaacaagcg
     7381 attcagggcg cagcaaccat gtatggtgtc ggcgttgaaa ctcgtctgat gggtgcagct
     7441 accgccagtt ctccttcgcc gcaatgggtc gcatggttgc aaagtcaggc agctcaggtc
     7501 ccgggggtca atcaagccat tgaacgtgtt gaagcgcctg cgggttccga agatgccaca
     7561 ttaatgatgg cccgcgtgca gcaacatcaa gggcaagcct cctatatggt gtttggcaca
     7621 cagctggcgg caggtcatca caacgaaaaa ttcgattttg acgagcaggt tctcgctatt
     7681 gccgtcgaaa cgctggcgcg caccgcgctc aattttccct ggacgcgagg tatctgatgc
     7741 aggaaatcta tcgttttatc gacgatgcga ttgaagccga tcgccaacgt tataccgata
     7801 ttgccgatca aatctgggat catccagaaa cacgttttga agagttctgg tcagcggagc
     7861 atctggcttc ggcgctggag tctgcaggct tcaccgttac ccgcaacgta ggcaatatcc
     7921 caaatgcctt tattgcttcg tttggtcaag gcaaaccggt tatcgccctg ctgggggaat
     7981 atgacgccct ggcaggttta agtcagcaag caggttgcgc gcaacctaca tccgcgacgc
     8041 ccggtgaaaa tggtcacggt tgcggacaca atttgctggg aaccgccgcc tttgccgcag
     8101 caatagccgt caagaaatgg ctggaacaat atgggcaatg cggcacggtg cgcttttatg
     8161 gttgtcctgg cgaagaaggc ggctcgggta aaacgttcat ggtccgcgag ggggtatttg
     8221 atgatgtgga tgcggcactc acctggcacc cggaagcctt tgccggtatg ttcaataccc
     8281 gcacgctggc aaacattcag gcatcatggc gctttaaagg gatcgcagca catgccgcga
     8341 attcccctca tttgggacgc agcgcccttg atgccgtaac gttgatgacc actggcacca
     8401 acttcctcaa cgaacatatt attgaaaaag cgcgcgtaca ctatgccatc acagatagcg
     8461 gcgggatctc gcccaacgtg gtccaggcgc aggcagaagt gctttatctt atccgcgccc
     8521 ccgaaatgac cgatgtgcag catatttatg atcgggtcgc caaaatcgcc gaaggtgcgg
     8581 cattgatgac cgaaaccacg gttgaatgcc gcttcgacaa agcctgttcc agttatctcc
     8641 cgaatcgcac cttagaaaat gccatgtacc gagccctatc ccattttggt accccggaat
     8701 ggaactgcga agaactggct tttgcgaaac aaattcaggc tacgctcacc cccaacgatc
     8761 ggcaaaacag tctgaataat atcgctgcaa ccggtggcga aaacggcaag gcttttgcac
     8821 tacgtcatcg tgaaacggta ctggcgaatg aagtcgctcc atatgccgcc accgataacg
     8881 tgcttgcggc atcgactgat gtcggcgacg tcagttggaa actgcctgtt gcccagtgtt
     8941 tcagcccctg ctttgccgtc ggtaccccgc tacatacgtg gcaactggtt agccaggggc
     9001 gaacatctat tgctcataaa ggaatgctgc tggcggcgaa aactatggca gcaaccacac
     9061 ttaatctctt cattgattca gggctattgc aagaatgcca acaagagcat cagcaagtta
     9121 cggacacgca accgtatcac tgccctatcc cgaaaaacgt gacaccgtca cctttaaaat
     9181 aacaacaaca acgcaaacac aacaaacgag gaatgcccat gagtatgtca tccataccgt
     9241 cgtcctccca atccgggaag ctctatggct gggtcgaaag aattggtaac aaggttcccc
     9301 atcctttttt gctctttatc tatttgatta tcgtactcat ggttacgacg gcaattttgt
     9361 cggcctttgg cgtcagtgcg aaaaacccga ccgatggtac gccggtggtc gtgaaaaacc
     9421 tgctcagtgt ggaaggatta cactggtttt tacccaatgt tattaaaaac tttagcggtt
     9481 ttgctccact tggcgcgatc ctggcgctgg ttttaggtgc cggtctggcg gagcgcgtcg
     9541 gcttactgcc agcgttgatg gttaaaatgg catcgcatgt taatgcccgc tacgccagtt
     9601 atatggtgct gtttattgct tttttcagcc acatttcttc cgatgcggcg ttagtgatca
     9661 tgccaccgat gggtgcactg atttttctgg cggtgggcag gcatccagtt gcaggtttac
     9721 tggctgccat tgcaggcgta ggttgcggct ttacggctaa tttactgatt gtcacaaccg
     9781 acgtgttgct gtcggggatc agcacggaag cggcggctgc gttcaatccg caaatgcacg
     9841 tcagtgtaat tgataactgg tattttatgg ccagctccgt agtcgtactg acgattgttg
     9901 gcggcctgat aaccgacaaa atcatcgagc cacggttagg tcaatggcag ggaaacagcg
     9961 atgagaaact gcagacattg accgaaagtc agcgttttgg tttacgcata gcaggtgtcg
    10021 tatcgctact ttttattgct gcgattgcgc tgatggtgat cccggaaaac gggatattgc
    10081 gcgatccgat taatcacacc gtgatgccat caccctttat taaaggtatc gtgccactga
    10141 tcattctttt tttctttgtg gtctcgctgg cttatggcat cgctacccgc acaattcgac
    10201 gtcaggcgga tttaccgcaa ttaatgattg aaccgatgaa agagatggcg ggatttatcg
    10261 tgatggtttt tcccctcgcc cagtttgtcg ccatgtttaa ctggagcaac atggggaaat
    10321 tcatcgccgt ggggctgacc gatatactgg aaagttcagg gcttagcggc atcccggcgt
    10381 ttgtcggtct ggcgttgctt tcctctttct tatgcatgtt tatcgccagc ggttccgcaa
    10441 tctggtcgat tctggccccc attttcgtac cgatgtttat gctacttggc tttcacccgg
    10501 catttgcgca aatcctcttt cgtattgccg actcatccgt attgccttta gcgccggtat
    10561 ctccttttgt tccactgttt cttggattcc tgcaacgcta caaaccagac gcgaaactgg
    10621 gtacttacta ttcgttagtt ttgccctatc cacttatctt tttggtggta tggctgctga
    10681 tgttgctggc gtggtatctt gtcggtctgc cgataggtcc ggggatttac ccacgtttgt
    10741 cttaagagag aacggatgct gagattactt gaagaaaaaa ttgtcacgcc actgggtcca
    10801 ctgtgggtga tttgcgatga gcaatttcgc ctgcgggcgg ttgaatggga agagtacagc
    10861 gaacgcatgg tgcagctgct ggacatccat taccgcaaag aaggctatga acgcatttct
    10921 gccaccaacc caggtggttt aagcgacaag cttcgtgaat attttgccgg taatcttagc
    10981 attattgata cgcttcccac tgccacgggg gggacgccat ttcagcgcga agtctggaaa
    11041 acactacgta ctatcccctg cgggcaggta atgcattacg gtcaactggc cgaacaattg
    11101 ggtcgccctg gcgcggcgcg tgccgttggt gcggctaacg gatcgaatcc catcagcatt
    11161 gttgtgcctt gtcatcgggt cattggcaga aacggtacca tgaccggata tgcaggcgga
    11221 gttcagcgaa aagagtggtt attacgccac gaaggttatc ttttgctgta aacattaaac
    11281 aatttgtgcc agcttgttca cacttttatg taaagttacc cttaacaact taagggtttt
    11341 caaatagata gacatatatt tacatctaat atcggaattc tctgctgtta aggtttgctt
    11401 agacttactt gctccctaaa aagatgttaa aattgacaaa tatcaattac ggcttgagca
    11461 gacctatgat cccggaaaag cgaattatac ggcgcattca gtctggcggt tgtgctatcc
    11521 attgccagga ttgcagcatc agccagcttt gcatcccgtt cacactcaac gaacatgagc
    11581 ttgatcagct tgataatatc attgagcgga agaagcctat tcagaaaggc cagacgctgt
    11641 ttaaggctgg agatgaactt aaatcgcttt atgccatccg ctccggtacg attaaaagtt
    11701 ataccatcac tgagcaaggc gacgagcaaa tcactggttt ccatttagca ggcgacctgg
    11761 tgggatttga cgctatcggc agcggtcatc acccgagctt cgcgcaggcg ctggaaacct
    11821 cgatggtatg tgaaatcccg ttcgaaacgc tggacgattt gtctggtaaa atgccgaatc
    11881 tgcgtcagca gatgatgcgt ctgatgagcg gtgaaatcaa aggcgatcag gacatgatcc
    11941 tgctgttgtc gaagaaaaat gccgaggaac gtctggcagc attcatctac aacctgtccc
    12001 gtcgttttgc ccaacgcggc ttctcccctc gtgaattccg cctgacgatg actcgtggcg
    12061 atatcggtaa ctatctgggc ctgacggtag aaaccatcag ccgtctgctg ggtcgcttcc
    12121 agaaaagcgg tatgctggca gtcaaaggta aatacatcac catcgaaaat aacgacgcgc
    12181 tggcccagct tgctggtcat acgcgtaacg ttgcctgatt tttccgcata actcactatc
    12241 cttctgtcat atcattaaat ttttctgatt tattgatctg gcagaaggtt catcactgtt
    12301 tcattcacca gatatgggtt aatcttttaa ttacaaactg cgttgacagt tgttgtaagg
    12361 agaccctgta tggctatgta tcagaacatg ctcgttgtta tcgatcctaa ccaggacgac
    12421 caaccagcat tgcggcgagc tgtttattta catcaacgga ttggtggcaa aattaaagcc
    12481 tttttgccga tctatgactt ctcatacgaa atgactaccc tgctctcccc ggacgaacgt
    12541 accgctatgc gtcagggcgt catcagccag cgtacagcct ggatccacga gcaggcaaaa
    12601 tattatctca atgctggcgt tcccattgaa attaaagtgg tctggcataa ccgtcctttc
    12661 gaagccatca ttcaggaagt gatcagcggc gggcacgatt tggtgctaaa aatggcgcac
    12721 caacatgacc gtctggaagc ggtgattttt acgccaacgg actggcatct gttacgcaaa
    12781 tgcccaagcc cggtgtggat ggtgaaagac cagccgtggc cggaaggggg taaggcgctg
    12841 gtggcggtga atctcgccag tgaagagccg taccataatg cgctcaatga aaaactggtc
    12901 aaagagacga tcgaactggc ggaacaagtc aaccataccg aagttcatct ggttggcgct
    12961 tatccggtaa cgccaattaa tatcgcgatt gaactgccgg aatttgatcc gagcgtttat
    13021 aacgatgcca ttcgtgggca acatttgctg gcaatgaaag ccctgcggca gaaattcggc
    13081 attaatgaaa acatgacgca cgtagaaaaa ggtctgccag aagaggtgat tcctgatttg
    13141 gcggagcatt tacaggcggg gattgtggtt ctgggcacgg tcggacgcac cggtatttca
    13201 gcagcattcc tcggcaacac ggcggaacag gtgattgatc atcttcgctg cgacctgctg
    13261 gtcattaaac ctgaccaata tcagacaccc gttgaactgg atgacgaaga agacgattaa
    13321 cgactcttca ataactgaca aggcccgagt taacgggcct attttttact tactgctctt
    13381 cgccgccaag gaaataaatt cccaacggga ttgccagtaa cacagtaaag accaggctat
    13441 aaacaaaaat catcgatgac tgaatcacat acattgatgt cgtccactca gacagcggaa
    13501 tattgtactg ttctatcacg ccgccgatgg tcgcgcgagt cacaaccgac gccgcaataa
    13561 tgaaaaccgc caacaaacca aagagaaatt tcttcccttt cgctgacttc agtttcgcca
    13621 taatcatgat gtaatccttg tgcagacaaa tttcttgtta cctaaacata acaataccat
    13681 gacattttac cgttgtctgc tctgaaaacc tcacctctta gctggattta caatcaacgc
    13741 attgattagt tagctaatgt tgctacttcc ttccctgact attgatgatc tgaatgaatt
    13801 gtcgcggtaa aatagccccg ccaggggttt ccggcatgac gacaattatc tggaggagcc
    13861 tgataaaaca atgatcgctg aactgtttac aaataatgcg cttaatctgg tcattatttt
    13921 cggtagctgc gcagcattga ttctgatgag cttttggttt cgccgcggaa atcgtaaaag
    13981 aaaaggattt ttattccatg cggtgcaatt tttaatctac accataatta tcagtgctgt
    14041 tggtagcatc attaattatg ttatagaaaa ctataaactc aaatttatca ccccaggcgt
    14101 tatcgatttt atctgtacgt ccctgattgc ggttattctg acgattaaac tatttctgct
    14161 gataaatcag tttgaaaaac agcagattaa aaaaggtcgc gatatcacca gtgcgcggat
    14221 tatgtcgcgt atcatcaaaa tcaccattat tgtggtgctt gttctgcttt atggcgaaca
    14281 tttcggcatg agcctttctg gcttgttgac ctttggtggt attggtggtc tggctgtcgg
    14341 tatggccggt aaagatattc tgagtaactt cttttccggg attatgctct atttcgaccg
    14401 tccttttagt attggcgact ggatccgttc accggacaga aatatcgaag gtacagtatc
    14461 ggaaattggc tggcgaatta ccaagattac gacttttgat aatcgtccgt tgtacgtacc
    14521 gaactcgctg ttttcgtcaa tcagcgtaga aaacccagga cgaatgacca accggcgcat
    14581 taccacgacc attggtttac gttatgagga tgcggcaaaa gtgggcgtta ttgtcgaagc
    14641 cgtacgtgag atgctgaaaa atcacccggc cattgaccag cgacaaacct tactggttta
    14701 tttcaaccag tttgctgact cctcattgaa tattatggtt tattgcttta ccaaaaccac
    14761 ggtatgggct gaatggcttg ctgcacagca agacgtttat ttgaagatta tcgatattgt
    14821 acagtcacac ggcgcggact ttgccttccc cagccagacg ctgtatatgg ataacattac
    14881 accgccggac cagggtcgct aaatcagaca tcactggcgt tatccacaag ggtaacgcca
    14941 attcccatca catcaatgct tcacaatata catagtccga ctgtacgcca catcttccgg
    15001 gttattaatg ggataacctt tcagccacgg cttgattaat cgtccattgg tatattgata
    15061 aattggtgca atcggtgctt gctccatgag gattttttct gccgcattgt aatcggcatt
    15121 acgcgcttta acggtatttt ccgttgatgc ctgggccagt actttgtcgt atgccggatt
    15181 gttaaagcgt gaaatattcc ctgaatgcgt tgacgttaac aacgtaagga aagtggaggg
    15241 ttcattataa tcccctaccc atgaggcgcg gataacatca aaattgccgg tgttacggct
    15301 atcgatatag gttttccatt cctgattctg caatttaaca tctacgccaa ggttcttttt
    15361 ccacatcgat gctacagcaa tcgcaatttt ttgatggttt tctgaagtgt tatacaaaag
    15421 cgtcagcttc agcggttttt gcggaccata accagctgcg ctcaacaaag tttttgcctg
    15481 cgcattcagt tcttcctgac tcatttgttc aaacggcgaa ggctccggcg taaaaccggc
    15541 agtaacatct ggtgtaaaat gccacgctgg cttttccccc gtccccaaca ctttttcggt
    15601 catcaggcgg cgatctatcg tcatacttaa tgccaggcga acacgctgat cggccgtcgg
    15661 ccctttttgc gtgttaaacg cataataata ggtcccgagc tgcggcggcg tataaacctg
    15721 ccccggaata tccttcaaca gcttctgata catatttttc gggaaggatt cggtgatatc
    15781 aatatccccc gcgagataac gcttagtggc tgcggattcc tgattaattg gcaggaaggt
    15841 cactttttgc agcaccgttt tggcgttatc ccaataatgg gtattcggta ccacgaccag
    15901 tttttcattg actacgcgct ctttaagaac ataagcgcca ttaccgatca gttttccggg
    15961 tttcgtccac tctttactac tttctacgtt ggctttttgc accgggaaga aggcaaagtt
    16021 agcggttaaa ttcacaaacc acggcaacgg tttatcaagc tggattttca aagtatgggc
    16081 atcaactgcg gtgacgccaa gctggtcagg cgtggcttta ccatcaataa tcgcctgtgc
    16141 gttgttgatt cccgccagcg cggcaaacca tgcaaatggc gacaatgttt ttgggtccac
    16201 cagacgttgc cagctgtaga caaaatcttg cgccgttacc ggtgtgccat ccgcccattt
    16261 tgcgttatcg cgcagggtaa aagtccaaat acggttgtca ttacttttcc actgagtcgc
    16321 aacgccgggg acaatctccc ctttctcgtt ctgattcacc agtccttcaa acagatcgcg
    16381 aatgacctga atctccggca ggcccacggc tttagcggga tccagagacg ctggctcatc
    16441 tttaatgtgg cgcaccagct cctgcttctc tgccagtact gtgccgctcg gaacttcagc
    16501 agcatacgaa agagaaatgc tgctgaccaa cagcgcacaa cacgtgactg aaacagagtg
    16561 cttcataagt ttccctctaa ccgacattaa aattgatgcg taattatttg tttcgcctta
    16621 aagaaatgca aacaacttcc atcaaaaaag tgacaaagga agcatcaaat gttttaagta
    16681 tcaatatatc aaatcagatt tagtgtaaat aatagtctcc aaaatgcggt aaagcacata
    16741 gaactccgaa cactacgttc gggcttcgca cgttcaaacc cgttttcggt atcataatca
    16801 gcatctgtcg ccattgtacg aagaataaaa aatgaccaaa acgttgaaag gaactgagaa
    16861 tacgttaaga gaagaattaa attaatgtat cagcaagtag tgctaaacag cacctttata
    16921 cttcagccta tctataaata atgagaatgc cgagccagca tgtcgacggt gtggatagta
    16981 caggtgataa ccgggtaaat ctggtgtgaa tttatccaaa acacgtatca gttttttttc
    17041 tttaatagcc cgctcaacct gatcataagg tagatacgcc aatccatgcc catcaattgc
    17101 agcatcaatg atcaggtcta tcgtattcag taaaagctga ccttccatgc gaacacgaac
    17161 ttcacgtcca ccgcgtatta atctccagcg atttgctgta cccgatgtgg gaagatacaa
    17221 attaattgcc tgatgatcta ttaattgtga cactgacgtt ggagcacttc gacgagaaaa
    17281 ataatccggt gagccaacaa tagccattgg tatatctggc ccgattcgaa tggcgatcat
    17341 atctttatcc atctccccac ccagacggac gcctgcatca aaacgttcag aaacgacatc
    17401 ggtcaaacca taatcaatgg taagctggat atcaatttca ggatgcgatt tcaggaatgt
    17461 gcgcatagct ggtaacaata tcgtttttgc tgcatgttct acagtagtaa tacgtattgt
    17521 cccggatggg cggttctgca gatcgctcag ggatgtcagg gcagaatcta tgtcatgcaa
    17581 catagggcca agaacagaca aaagatgctc gcccgcttca gtgggtacaa cgctgcgcgt
    17641 ggttcgtgtc agaagccgca atcccaatcg ttcttctata cggcgcacta tctggcttaa
    17701 agctgactgc gccatgctca ggcgggctgc tgcgcgagtg aagctacgct cctctgcaac
    17761 gacgacaaac gccatcagat cagcgatttc ttcacgcttc attcagtatc tccatacaat
    17821 ctattaatca tttaaatgat aggtctaatc atgattttgg gtctaatcaa cttaatgatc
    17881 aatatctata gtgatatcac caaccgttaa aacgaaccga caactccatg aggaacggaa
    17941 tgatgaataa taaagtcagc ttcactaaca gcaataatcc aaccatctct ttgtctgcag
    18001 tgatctattt ccctccaaaa tttgatgaaa cccgtcagta tccggcaatt gtggtatctc
    18061 acccaggtgg gggcgttaaa gagcaaacgg ctggaaccta tgccgaaaaa ctggcggaaa
    18121 agggatttgt cactattgct tatgacgcat cttatcaagg tgaaagcggc ggcgagccgc
    18181 gtcagttaga aaacccatac attcgtactg aagacgtcag tgccgtgatt gattatctga
    18241 caacgctttc ttatgtcgac aatagccgaa ttggtgcgat gggaatttgt gccggtgcag
    18301 gatatacagc caatgctgca attcaggatc gccgcatcaa ggccatcggt actgttagtg
    18361 cggtcaatat tggttcgatg ttccgtaatg gttgggaaaa caatgtgaaa tctattgatg
    18421 ccctaccata cgttgacgcg ggttcaaacg cccgaaccag cgatattagc agcggagaat
    18481 acgccaccat gccactggca ccaatgaagg agtccgatgc gccgaacgaa gaactgcgtc
    18541 aggcctggga gtattaccac acccctcgcg cgcagtatcc aacagcaccg ggttacgcta
    18601 ctttgcgcag ccttaaccag attattacct atgatgctta ccatatggcg gaagtgtacc
    18661 tgactcagcc gatacagatt gtggcgggca gccaggcagg gagcaaatgg atgagtgacg
    18721 acctgtacga tcgagcatca agccaggata aacgctatca cattgttgaa ggcgcaaacc
    18781 atatggatct ttatgacggc aaagtctatg tggctgaagc aatttcagta ttagcgccgt
    18841 tctttgagga aactctgtaa tgaaacatat tcaaatccgt aatagtgata tggcctggca
    18901 tattgctgcc aataatctgg gaaaagtttc atgaaaaatg tactgattct tggtgctggc
    18961 ggtcagatag cccgccatgt gattaatcaa ctggcagata aacaaaccat taaacaaact
    19021 ttatttgccc gtcagccagc aaaaatccac aaaccctatc caactaatag caagattatt
    19081 atgggggatg tactgaatca tgcagcactg aagcaggcca tgcagggaca ggatgttgtg
    19141 tatgccaacc ttacggggga agatttagat attcaggcaa attcagtgat cgctgcgatg
    19201 aaagcctgtg atgttaaacg tctgatcttt gttctttccc tcggtattta tgatgaagtt
    19261 ccaggaaaat ttggcgaatg gaataatgca gttatttgtg agcccctaaa accattccgc
    19321 agggctgcgg atgcaattga ggcttctgga ctggaatata caattcttcg ccctgcctgg
    19381 ctgaccgatg aagatattat tgattatgag cttacctcac gcaatgaacc attcaaaggt
    19441 acgatcgtat cacgtaagag tgtcgcagca ttaattacag atatcatcga taaacctgag
    19501 aaacacattg gtgagaatat aggcatcaat cagcccggga ctgatggcga taagccattc
    19561 ttcatgtaaa tcaattatat agctaccttc gtactgtact atgtgcagta cgatttatta
    19621 aacttattaa ggtgaactga tcaatcagat gcgattaaca tgtgtagaac cagtaagaat
    19681 aaaatacgcc atgtatataa cattacgaca attctaatat ctgcacatca atgaaaatgc
    19741 aaacaacttc cggcaaaaaa gtgatacaag atcgatgaca tagcgttgaa gaaaaggatt
    19801 tgataatccg caaaatttgc acaacactgc ctgtaatact tcctgaacca gagacgatca
    19861 tgaccgtaac ccgcccacgc gccgaacgcg gcgcatttcc gcccggaaca gaacattacg
    19921 gacgttcatt attgggcgcg ccgttaatct ggtttcctgc ccctgcagcc agccgcgaaa
    19981 gtggtttgat tctggctggc actcacggtg atgaaaactc ttcggtcgtc acgctctcct
    20041 gtgctctgcg gacgttgaca ccttctttgc gccgtcatca tgtggtgctg tgtgtgaatc
    20101 ctgacggctg ccagttgggg ttacgggcca atgctaatgg tgtggattta aacagaaact
    20161 ttccggcggc gaattggaag gaaggtgaaa ccgtttatcg ctggaacagc gccgctgaag
    20221 aacgtgatgt cgtattgctg acaggtgaca aacctggctc tgaacctgaa acccaggcgc
    20281 tgtgccagct tatacaccgc attcaacccg cctgggtggt ctcattccac gatccgctgg
    20341 cctgtattga agatcccaga cacagcgaat taggtgaatg gctggcccag gcgtttgaat
    20401 tgcctctggt aaccagcgtc ggttatgaaa cgcctggctc tttcggcagt tggtgtgcgg
    20461 atctcaacct gcattgcatc accgcagagt ttccacccat ctcctccgac gaagccagcg
    20521 aaaaatatct ctttgcgatg gcaaacttgc tgcgctggca tcctaaagat gcaattcgcc
    20581 cgttgtgaac tgaagcgccg gttccacatc taccgccagc caggtcggtc cgtcaagatc
    20641 ggcgaaactg acctgcggca ccagcggtaa agcggcgcta atggcacgag aggtacacaa
    20701 catgcagccc agcatcagac tgaaaccctg tgcacgcgct tcagtcgcca gcgccagcgc
    20761 ttccgtcaga ccgccggttt tatcgagctt aatgttaacc atctcatagc gccctttcag
    20821 cgccttcaga ttgctacgag tatgacaact ttcatctgca caaatcggta acggatgaat
    20881 aaaattctcc aacgccgcat cgtcctgcgc aggaagcggt tgttcaagca ttgcaacgcc
    20941 taaatccgcc aatagctggc aacgcgccgc caacccttct gcacgccagg attcatttgc
    21001 atcaacgatc agcgtcgcat cgggcacagc ggtgcgaatt gccaccatcc gctcactgat
    21061 aagatggtta tccagcttca ctttcagtaa tttcgcgcca gcctgccaga gtgttgatgc
    21121 actattggcc atctgatcag gcgtaccgat gactaccgtc tgtgcagtga taactgtccc
    21181 gggaagcgtt atgccgatca aatcagccag cgattcctgc tgttttcgcg ccgcgagatc
    21241 ccacaatgca caatccagcg cattacgtgc tgcgccggca gggagaattt tttgcaactc
    21301 ctcccgtgtc agccctttct ctaattgcgg tacgacgctc ataatttgcg ccattaccga
    21361 ggcatcactt tctccataac gcggatacgg cgtgcattcg ccggtgcctt taataccttc
    21421 ttcttccagt tcaaccacca ccacgcgcgc ttcactgcga cttccccgag caatcacaaa
    21481 cggggtatgt aagggccagg cttcctcgaa taccttaacg gttctcatct ttactccttg
    21541 ttgtcgcggt ggtctgcgga ttaagcgcaa atagcgtttg ctgtgttatt gacagttagc
    21601 ataaactagg tgtgacgtta actatatgta aacaggaaag ataatcatgt cacaaaccgt
    21661 tcatttccag ggcaacccgg ttacagtcgc caattccatc ccgcaggcgg gtagcaaagc
    21721 gcagactttt actctcgtgg caaaagatct gtctgacgtc accctcggtc agtttgcggg
    21781 taaacgcaaa gtgctgaaca ttttcccgag tattgatacc ggtgtttgcg ccgcatcagt
    21841 acgtaagttt aaccaactgg caaccgagat cgacaacacc gttgtgctgt gtatttctgc
    21901 cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa ggtctgaaca acgttatcac
    21961 cctctccact ttccgtaacg ctgaattcct gcaagcctac ggtgtggcaa ttgctgatgg
    22021 cccactgaaa ggtctggcag cgcgtgccgt tgtggttatt gatgaaaatg acaatgtgat
    22081 tttcagccaa ctggtggatg aaatcaccac cgagccggat tacgaagcag ctctggctgt
    22141 actgaaagca taagttaata tgcctgatgg tgttgcacca tcaggcatat tcgcgcttac
    22201 tcttcgttct tcttctgact cagaccatat tcccgcaact tattggcaat cgcggtatgc
    22261 gaaacgccga gacgttttgc cagtttgcgc gtgctgggat aattgcgata aagctgggtt
    22321 aataccgagc gttcaaaacg gctggtgatt tcgtccagcg aaccttccat cgcatcttcg
    22381 cccacggcta ccgttgcggc gtcataatcc ggcaacaaaa tatcctgagg acgcagctca
    22441 taaccgtcca gttgtgttag tgcgcgataa atagcgttct ttaactgccg cacatttccc
    22501 ggccacgcat aacgcgtaag tacagtattc aggtcagcgg ccagtttcgg acgcggcacg
    22561 ccctgctcgt cggcaaagcg ggcgacgaac agctcggtta aaggcatgat gtcctgcgga
    22621 cagtcacgta gcggcggcag attgagcgtc aacacgttca gacgataata gagatcttca
    22681 cggaacacgc ctttttgcac cagttcgacc agattcttct gcgtagcgca aatcacccgc
    22741 acatcgacat gcacctcatg gtcttcgcca acccgacgga aagtgccatc attaaggaaa
    22801 cgcagtaatt tcgcctgcat ccgtggtgac atctcaccta tttcatccag cagtaccgaa
    22861 ccaccgttcg cctgctcaaa gaaccctttc ttcccttccg gagcatgacc aaacagttca
    22921 ctctcgaccg catcttccgg gatagaagca cagttcagcg ccagataggg tttgcccgcg
    22981 cgtgggcttg cctgatgaca ggcatacgca aagagatctt ttccggtgcc ggtatcaccg
    23041 gttatcagca atggcgcgct tagcatcgcc agtttctgcg cctgttcgac aacatgcttc
    23101 attttcgggc tgacggcgac aatttgacta aaggcgctga cgtcctgggc ggcgacattt
    23161 tgcaactgac gacccatacg aatcgttgat cgcaacatca ccaccgcacc ggtcaggacg
    23221 tgttgatcat tttcatcctg aagataaaca ggcgtaatct ccatcaggaa attctgccca
    23281 ttaataacga catgctcgtt atgcgaatcc tgcggttcgc tttccagcca acgcaaaaaa
    23341 ttaaagccgt taatcaattg cgcggcggta tggttgcgca ggcgatccaa tttttgccca
    23401 aaaagctgac agctcgccgg gttcgccata tccactttgc ttttcatatc gacagagagc
    23461 acaggttcag gcaacgcttc cagtaacgcg ctcaacgcca gatgctcacg ttcagaaggc
    23521 atccacggga cagtacggac atcggtaaca cctgcaatac ggcgtatttc ggccatcaga
    23581 ctgctgaaac tctcaaactc cagttcagca aaattgaggt agattcgccc aatggggtca
    23641 atctcaatac tgcgtaaatc aatgcctctt agcacgagta gatcgagtaa ttcgcgggtc
    23701 agaccgagtc ggtcttcaca aaagacttcc agacgcatgg gaaccttcac ctgaaaaaag
    23761 aacaattaat atgatgatat gacactattt ggatagcagg aaggtttctg tcaacaatca
    23821 ttgacacaaa gacggaaaaa cgggcacttc ggcgtaaaga tatccggtca gcacccgata
    23881 ttgcattatt tttcgctggg cgttttgcct ttttgcagcg tctctttcac ctgaccgata
    23941 agctgacgac ggaaatcccc gaggcgaggt ttgtcatcgt caatccacgg cagagggcgg
    24001 caaagctcca tagctttaat ccccagtcgt gccgtcagaa gtcctgcacc aatcccctga
    24061 gctgcccggg tagacaaacg cgcagcgaga tcttgcgaca tccagtccat ccccacttcg
    24121 cgcaccagtt cgctggctcc ggcgaaagcg atattcagca ataccagctt aaacaggcgc
    24181 aaacggctgt aatatcccag ttcaatgcca tacagcgtgg cgatgcgatt gatcaaacgc
    24241 agattgcgcc aggcgataaa tgccatatcc accagcgcca gcgggctgac cgcaatcatc
    24301 aacgttgatt ccgccgccga acggctgatt tcgcgccgcg cctgggcatc taaaaccggc
    24361 tggaccagat gagcatacag gctgaccact tcacgatcgt tttgcgtttc atggattgag
    24421 gcataccagc gttgcagtgc cggatgcgac tggtcgatgc ccgcctgctg cgccagtttt
    24481 tcgcaaaatg cgcggccttt acccgtaccg tgactgtgca acaaatcgcg cgcttcgtcg
    24541 cgttcatggg cgcgctgtcg caagcgccat aagcgccgcc actctgttac cacagaacct
    24601 acgccagcgc cgataatcaa tgccccagcg gcacatccac ccagcgctac ccaatcctga
    24661 gtttgccagg cgttcattgt ccactgcacg ccctgcccga caacgcttgc gccaaacaga
    24721 gccaacccgc ccatcaccat tttgcgccac aggctgcgtt tcggacgtaa cgctgcgtcc
    24781 atcaccgctt caacttgccc ctcttcttcc tgcgcttcgt cgagcgtggc cggggcaaaa
    24841 ttttgcgcct gattttcgtc aaaggtctgc tgcgccctga attttggatt ctgatcgacc
    24901 tccagtggac cgtcgaaatc aatacgtggt tttaacggtt cggtcatcgc aatttatctc
    24961 ctattaaaaa ttccagcgca gcatcaagac gaatatgcgg cagcggtttg tcgacatcca
    25021 tcacctgcgg acgaaacgcc tcaaactgga agccttgctt atcccagaac gcctgaccag
    25081 gcaaacgtgc gggaacttcg ccaggataaa cagtgagcgg tgcgccatcg ctgagtcgat
    25141 taccgcgcag cgccgggatt ttctcaccgt taacatcaat aatgccgctg gtggtcgcct
    25201 gaactgacgc cagccccagg caatccatgc tgatcccttc gaacgccgca ttttgccagg
    25261 catcctgaat tagttgttgc agtaatgaaa ccatattggc gtgctgatcg atggtcacat
    25321 ggtctgcttt agtagcagca aacaatagct tatcgataac cggcgaaaac aaacgccgga
    25381 acagggttcg ctgcccgtag tggaaacttt gcatcagctg cgtcagcgcc agacgcatat
    25441 cattaaatgc ctgtggccca ctgttgagag gctgcaggca atccaccagc acaatctggc
    25501 ggtcaaagcg cagaaaatga ttcttataga accccttcac cactttctcg cagtaataat
    25561 taaaccgctc gcgcagcatt ccggcattgg tatgcttatc ggcctgcgcc agtttggact
    25621 cgccccaggc atcgacatcc ggccacggga agaattgcag cgcgggtgct cctgccatat
    25681 ctcccggcaa gacaaagcgc ccaggctgaa taaagtgcaa cccctgctgt ttgcagtggt
    25741 ggagatactc ggtccaggcg gcggcaatgt ctgccagccg gttttcgtcg gcaggcgcta
    25801 gcgggtccag cccttcgcac atcattcgcc atttcgccga ccattcgccg cgctgaccat
    25861 tgagtaagcc cgtcatctgg cgcgaccagc ttaaatagtc ctgcgccagc atcggcaggt
    25921 cgagcaacca ttcgccgggg taatccacaa tttccagata cagcgtggag gtgtccttaa
    25981 agtggcgtag cagcgaatcg ttcgatttaa aacgtaacgc caggcgaatt tcactgacgc
    26041 cgcgcgtagg cgtcggccag gcgggaggat cgccatataa ctgcgccaac ccttcatcat
    26101 aggtgaagcg cggaatgcca aagtcacgct ggggaatgcg ttttacgccc agcaggcgct
    26161 cttcacgcac cgcacttaaa agcggcaaac gtgctccggc atgaatattg agcaactgat
    26221 taaccatcgc ggtgataaac gctgttttgc cgctgcggct aagtccggtt acagcgaggc
    26281 gcagatgtct gtcgacaccc cgattcacca gcgcattaag ttcattttta agtcgcttca
    26341 ttgctgtcct gtgctgctct gaagaccaga aaaaaatcat tcgtaaggat atgaatgtgg
    26401 gggccaggcc attgattatc aactgcccgt gttgtcatcg acgggcgaac tccataagat
    26461 gtgcaacatt cacgttcgga cggagttcct gacctatgtc ccctactatt tatgatattg
    26521 ccagggttgc aggcgtatca aaatccaccg tatcacgcgt gctgaataag caaaccaata
    26581 tctccccgga agcgcgcgaa aaagtgttac gggccattga agaattacag tatcaaccaa
    26641 acaagctggc acgcgcgctg acctcttcgg gttttgatgc cattatggtg atttctaccc
    26701 gttcgaccaa aactacggcg ggtaatccgt ttttctcgga agttttacat gccatcaccg
    26761 ccaaagctga agaagaaggt ttcgacgtga tattgcagac gtcgcacaac ctggcagaag
    26821 acttacaaaa atgcgaaagc aaaattaagc agaaaatgat taaaggcatt attatgctaa
    26881 gttcgccagc ggatgagtca ttttttgccc aactcgataa atatgatatt ccggtggtgg
    26941 taattggcaa agttgaaggt caatatgccc atgtttattc tgtcgatacc gataattttg
    27001 gcgacagcat tgcgttgacc gatgcgttaa ttgaaagtgg gcatcaaaat attgcctgcc
    27061 tgcatgctcc gcttgatgtc catgtttcgg ttgaccgcgt taatggctat aagcaaagtc
    27121 tggctgcgca taatattgca gtgcgtgatg aatggattgt tgacggcggt tatacccatg
    27181 aaacagcctt gcaagccgca cggcaattat taagccagtc gccgttgcca gaagccgtat
    27241 ttgccactga cagcctgaaa ttaatgagca tttatcgtgc ggcagcagag aaaaatattg
    27301 ctattccgca gcagttagcg gtggtgggtt atagcaatga aacgctgtca tttattttaa
    27361 cgcctgcacc gggcggcatc gatgttccga cgcaggagtt agggcaacaa agctgcgagt
    27421 tattattccg cttaatttct ggaaaaccgt caccacagaa tattaccgtt gccacgcata
    27481 tgacgctgaa ataaatacca gctaccttgc ctgaatgccg gatgcgatgc tagcgcatct
    27541 tatccggcct acatttatag atcggataaa gtactcgcgc cgcatccggc aatttaaccc
    27601 attatcagaa cgagtaattt acgccgacac ctgcataatg gaatttatcg ctgtcgcctt
    27661 cgtcgtgatc ctgccactca aacgcgtatt ccagcgaaac ggaaagacca ttctggaaat
    27721 cataaccgta aaataacccc acacggttaa aatcatggcc ttcacgttca atatcatcct
    27781 gccagtccca gttactccag cgatccagcc caatgcgcgt atacggcgtc accgagtggt
    27841 tgccgagcgt cagcggcaaa taggcacgaa tttcttgcgt ggaaaactca ccgttattgc
    27901 ggctgtcgtc catattgaag ccgcgctcga gataatagtt cactcgcaaa gcaaccgttt
    27961 cgttgaaggt atattgcaga cctgtttccg tttcgactcg ggtatcagcg taaccggtcg
    28021 tgttcagatc gttggcaaat ttatacatcg ataaccaacc gttgaaacgt aaatcgtcag
    28081 tcagtttcac atcccagtct ggcgcgattt tccagcgctg catattcgcc gtgtctttac
    28141 ccggttcatc aacgtagtga taaccataat tacgaaaacc gccggtcagg ccgaaactga
    28201 aatcatcgct ttcgaggaac tgataatgca cctccagctc cgggcgatca aaccatgttc
    28261 cacgtttacc cgcgctataa tctaccggcc cttcctgata ataagccaga gaaattctcc
    28321 acggcccgtt ggcggcatta aaatagacag aaggctccgc gagcccatcc atatcttcgc
    28381 cataaccctc gacgttttct atttcgtaca tcgcgccgat attaaagtgc cagtcgttct
    28441 tttcctcggc ctgtgcgcag gccattcccg cacacatcac cagtgcggta cagggtaata
    28501 actttttcat tattgttcct tgtattatcc tgggggaatg ccttccccct gtatttagcg
    28561 aattgctatt tccgtttctg catcaaagaa atgacattta gtcatatcaa aatgaatagt
    28621 gatattttct cctgcatgat aatcatttaa cgcaccagca cggaccacta actcgtgccc
    28681 cccaaccgtg gtgtagagca taaattccgc accggttaat tctgccacgc taattttggc
    28741 ggaaatgtta ttttcctctt gcgcgtccgg atgaatatct tccgggcgta ttcccatcac
    28801 gatgggctta tgcaaacttt cctgtgtttt cagaaccgct aatttctctt cgggaatggt
    28861 taatttaagc gtttcagtaa cgaatttatc gccatcgatc gtgccgcgaa taaaattcat
    28921 cgccggtgat ccaataaatc cggcaacaaa catattcgcg ggttggttat aaaccgtttt
    28981 cggcgcacct acttgctgaa caatcccatc cttcataatc acaatccgcg tcgccatggt
    29041 catcgcttcg gtctgatcgt gggtcacgta gatcatggtg gtgttcagtt tctgatgcag
    29101 cttgctgatc tctgcgcgca tttgtacgcg cagcttggca tcaaggttag agagcggttc
    29161 atccattaaa aacacgcccg cttcacgcac aatcgcccgc ccgagcgcca cacgctggcg
    29221 ttgcccgcca gaaagagccc ccggcttgcg tttcaggtac tcacgcaggc cgagaatttg
    29281 cgccgcccag ttcacccgct catcaatcac ctctttggcg attttttgca tcttcaggcc
    29341 aaacgccatg ttgtcgtaga ccgtcatatg cggatacagc gcgtagttct ggaacaccat
    29401 cgcaatattg cgcgctttgg ctggaacgtc attcattcgt ttgccgtcga tcaacagatc
    29461 gccgccgctg atctcctcaa gcccggcaat catgcgcagg gtggtcgatt taccgcagcc
    29521 tgacggaccg acaaagacaa tgaactcttt atcggcaatt tccaggttga agtccttcac
    29581 cacatgcacc tggttatcgt agattttttg aatatgttgt aacgaaagct gagccatgtt
    29641 gattcctttg ctatacgttt tgccagaagg ccgataaccg cggccaggtc agggagtccg
    29701 ttgagggcaa caataattgc gccccggtta agcccgcgcc gatccccacc gagcgcatac
    29761 cgctggcgtt aatggcgtca atgcccgcct gcgcatcttc aataccgata catgcctgcg
    29821 gcggcacgcc cagtccggca caggcggcga gaaagatttc cgggtccggt ttcgagtttt
    29881 taagttggga agcgtccgcg cagaaggtga aaaactcgcg cagctccagc gctgctaaaa
    29941 tcgtcggcgc attcagggag acagaagcca gccctatagg gatctgctgt gcgcagagat
    30001 ctgtcagcaa agagcgaatg ccgggcagca cagcattaac cgtcagttcg cgcagtgagt
    30061 ggacgtagag cagatttttg cgatacgcca gttgcgccct ttcctgcggg ttaaagtcgc
    30121 cctctttgcc cccgtgttgc agaatgcgtc gcagagactc atcgcggctg atccctttta
    30181 gggattcgtt aaactgcgca tcaatgctga tgccaatttc agcggcaatc tgctgccacg
    30241 ccttgaaatg cagatgcgcg gtatcggtga ttacaccatc cagatcgaaa attacccctt
    30301 gcagtttcat tcatcctcct gatgcgtggt agcggtccca ttgaagggca aaataaaatc
    30361 acccaaacag aaaacagatt cttcagccac agatataagc tgcccgttca aacgcagtga
    30421 aacgggcgca gaagttcgaa tcgcaatacg ctgcgcgtcg agagtgacct gtaattcgca
    30481 gccctgccag aacagaggga aagacaactg ttgccactgc acaggtaacg ccggattcaa
    30541 atttaattca ccgtcacgca cgctcacccc ggcaaaaccc tgaatcgccc ccagccagat
    30601 agcgccagtt gcggcagcat ggataccgtc gtcgcagcta tgcgggtcag caccgagatc
    30661 gatttcagtc ccctcccgcc agaactgata actttgcgcc aacaggccac agcgtgcggc
    30721 aacaatgtcg tggatcgctt tactcagtga agagtcgtaa atggtgcgcg gttcataaaa
    30781 ttgcagattg gcaagacacg atgccgctga gtactgctcc ggcagcatgt aattgagcat
    30841 caccacatca gcttgtttga ggatctgcat ctcgttcact tctgcgcgtg aataatccag
    30901 cagaatggtt tgcttccccg ccgccgcttt gtatttcgcc agattaatcg ctggtttcgc
    30961 cataaacgaa tcatcctgcg gcaaaacgcc gtcgggctga atttctggca gccgcagttc
    31021 tttaaggaac atttcggcgc gatggataaa cgcatcgtcg ctacagccaa attgacgagc
    31081 gatgttcagc gcctgctgga cgttgtaata cgccatatag ctggtgaagg cgttattatt
    31141 gacatgttcg gtatattcgt ctggcccaat aacatcatga atttccagac ggtcgttaac
    31201 cctcaccgtg cggctaatcc agaactttgc agtttccaga agtagcgcca tgccttcatg
    31261 agcaatgaaa ctttcatccc ccgtggtctg ccagtattga acaaccgccc aggcgatatc
    31321 ggcgaccaga tgatgttccg cctgcgccga ggccactttt tgccgcagcc cggtgcgaat
    31381 gttaatggcg gcaaattccg gcgtctcttc ttcgccgctg cgcgcgcttt cccacggaaa
    31441 tagcgcgccc tgccagccgt tgcgtcgcgc tttctcctgc gcgcctggca agttgtgcca
    31501 gcgataacgc agtaaacttc gggcaaccgt cggatcgcta aacagatgaa acggtagcaa
    31561 aaatacttct gtatcccaga aaacgtggcc tttgtagcct tccccggtca aaccttttgc
    31621 cgcaatactg ctgcgctcgt cgtgagcagg cgtcatgatg cgcagatgat aaagcgcata
    31681 atctaacgcc tgctgattgt gctcatcgtt actctttacc gtgatacgac gtttctgcca
    31741 ccattgacgc cagttttctg tggatgctgc aagaagttgg tcgtaactct gctgtacaca
    31801 catttcaagc tgacgaagcg atgcactgcc ccactcgtct aaagcgacgt gtctgtcgtc
    31861 ccgccagtcg aaccacacca gtttttgcag cgtcagcgtt tcgcctgcgt gaagttgcgc
    31921 actggtatgt tgcagcaagc ggcgctcttt ggcggtatag cattgctgca catcaccgct
    31981 caccttacag caacagctga tggccacatc actgcgtcca tcctgagtgg tatagatccc
    32041 ctgcatcaaa tgctgaccaa acacccgcac ctgggtttcg tcgagatgtt gtcgtccgtg
    32101 gttggtttgc gtggcgtcaa tgcctgttga aatcagcact gacgcgtcag cgtccagcgg
    32161 agtaatagtg atttccagcg caatcagcgg cagttggtct gccgaaacaa agcgacggct
    32221 gacgattgtg taacgcgcgc cgttactggt acaccagaca acgttgcggc gtaattcgcc
    32281 actggcaaag tcaagctcac gctgccatgt ttcgcgggat aacgcaaaaa cctcaccatt
    32341 tatggcaatc tccatcccca cgacatcagg caggttcacc agttcgttga tttcaccctt
    32401 acccgcccga tgatacagcc ccgccagata catcccacgg gtctggcgcg tgtaatcttc
    32461 ttcatggctg gcgcgaagcc caagatagcc gttcccctgc gccatcagtg aagcatattt
    32521 gttcagtgta tgctggctga aatggggttc tgataacgtt actggcctgg tcataattcc
    32581 acacgcgtcc ctgtttctgc ggattgatac aacgccgcca ccagttgctg gatgatgtac
    32641 ccctgctcgg cgtcggctat catcacgggc ttgccctgta cgtgattgat aaaggcttcc
    32701 atactgcgca aatggcggtt gtcgtctgcc atttcccgtt gcatcagcgt cattaactca
    32761 ccgttgttat cggtatagat atgcgccgga aacagcgtcg cccctgcttt atcgccacaa
    32821 aagctgacgt tcataatcga ctgttcgcgg atgttgagtg caaatgacgt ttccagccac
    32881 agaatgccgc cgttatgaaa ttcaatggtg ccaaacagcg aatcttcgac gctgtaagtt
    32941 gccgggtccc actcgccaaa ttgaccgcag ctcttttgcg tgccgatctt ttgaaagcta
    33001 tgcgcagtca cgcttttcac cgctggaaaa cccagcacat acatcgccgc atccagcata
    33061 tgaatgccga tgtcgatcag cgggccaccg ccctgcaatt ctttattggt aaagacaccc
    33121 cagccgggaa caccgcaacg acgcagggcg cgggcggtgg taacgtaaat ctctcccaaa
    33181 acgccgttgg tcacctgttc acgcagctgt tgcgtatcga gcgcaaaacg atggtgaaag
    33241 tcgtaggcca gcaccttgcc ctgtttgcgc gcggtatcgc acatttcccg cgcctgttct
    33301 ggcgtcatgg cgggcggttt ttcgcacatc acatggcagc ccgcttccag cgccatcagg
    33361 gtatgttcgt aatgaaaacg gttaggtgag cagacgctaa ccacatcagg tttcaccgcc
    33421 agcagcatgg cctgcggatc gtcccacacg gatgcattcc cgtatttttc tgccagcgcc
    33481 tgcgcctggg aaaggcggct gtcacagaca gccaccagtt ccagatcgtt gcgggtgcag
    33541 tagtacgaag catgaacctt atccgccacc tggcccgcgc ctatgatcgc gacccgcagc
    33601 ggagagcttg tcattgcact tttcacaaag acctcttagc aggtacgcaa ccaggccaaa
    33661 gaatcacggt acgcctgggc aggatcttct gcccggatac gcccttcgta caccacatag
    33721 ccctgatagt tatccgcgcg cagctgttca aacagcgcgt ggaaatccag ggtgccgctg
    33781 cccggctggt agcgatggtt atctgcaata tgcacatgac cgagcaggtc gcggttgtca
    33841 tgcagcgcct gcgccaggtt atcttcttcg atattcatgt gatagaaatc gccgataatc
    33901 tgtacatgtt taagatcgtt ttcgacgatg taacgacgag catcggcgag ggtgttgatc
    33961 atgtgatcct gatagcggtt taacggttcg aggtacacca cggttccggt acgcgctgcc
    34021 acctgttcca gtacgcgcag ggaatcactc accattttgc ggtcgccatc caggctgcgc
    34081 ggcgaggcca tcggcggtaa acggaaggta aacatgcccc acgctgccgg aacgacgata
    34141 cctttaccgc ccacttcggc cagcgcttca agaatgcgtt cgatctgctt taagccatta
    34201 agacgacgct cttcgataaa gtcgccaatc cagccgtcat agccgccgca ggcggtggtc
    34261 accggtaaac cggtttcttt gatcgccgct ttgacttctt cgatgttgtt gaccagaagt
    34321 ttgccgtcaa tctcaaaacc atcgaagccc atctctttga tataacgaaa tttctccaga
    34381 atattttccg gaaagaacgc ctgattctgt gtgccgattt tcattttttt cattcctttg
    34441 aattaaaacg tgacgcccat tttgatgctc agttccggat gctggtcgac atactgcatg
    34501 tagctttccg ggctattggc aaaggtcacc accgggtcga ttaaatcttc gcaattgaga
    34561 taaccgttca tcagcagttc ccagcaggtt tcttcgatac gcttgcggct ccagcgcgga
    34621 taatccgggt tcggttcgct gcacgcgcgg gagaagacaa tttttgcgtt attgaaatgc
    34681 gcttcgcgtc cgaggttaaa accttcggca aacggtttgg caaacgcgac ataggagatg
    34741 gtgccgccat aagccagacc tcgtagcgcc gattgcagcg cgtcggcata gccgctggtt
    34801 tcgataatca catcggcacc ctgcttgccg gttagcgttt tgatctcttt gcctacgtca
    34861 gtgccaatgg ggttaaggca gaaatccgcg ccgtggcgac gggcaatatc acagcgatag
    34921 gcgataggat cgacgccaat caccaccgac gcgccagcgc gtttcgccag ttggatggct
    34981 atttgaccaa tcgcgccaag ccctaccacc accacaaaat cacccacgcg cacgttggca
    35041 tcgcgcacgc cgctcatggc aaactgcgcc gggtcgtaac aaacggcgtt tttccacgag
    35101 ctgccttccg gcattttgcg cagcttgtag ttattcaccg cgttaatgat gaccgtctcg
    35161 gagagcgggc cgtagccgca tacgctgtcg cccaccgcgt agtcggtaac gtcgctgccg
    35221 cactcgataa tgtcgccaac caccatgttg ccaagctgga atttgccaaa ctcaatgccg
    35281 cgcggcgcat ctgccggacg cggagtgaac atctgccatt cgccgttaaa gtcttcatcg
    35341 ataaacgggc tggcggcgcg gaagtcgacc acttccgttc cgtgtttcgg tgcgccgaaa
    35401 cgcacgcgga ttttcacttc attggctaaa atcgcccgat cttcatactc aaccagcgca
    35461 gcaacacgcg gtgctgtggc tactaacttt ttcatttaaa actccttgta aagaaactgg
    35521 ccgctttagc ccttcacgcc accggcggtc aaaccactct taataaaacg ttccgaaagc
    35581 gcatacataa tcaccaccgg caatgcggtc accagtgagg ccgccatcat ccgtccccag
    35641 atgtaatctg gcgtactgaa cagcgcgttc aggcccaccg gtaaggtgaa attgctggcg
    35701 ctggagagga aaatcgacgc aaacagatag tcgttccacg ccaccataaa gcagtagacg
    35761 aacacggata tcagcccgga catcgccaac ggcacggtga tgcggaagat aatttgcaga
    35821 cggttgaggc catccatcat cgccgcttct tcgatctcat cggggatggt gtcgaagtag
    35881 cttttcagca taaacacggc ggtgggcaga gtttgcgtca ccatggtgat gatcagcgcc
    35941 atctcggtgt cgtaaatgcc cagcgcggtg atgattttga acagcggcac caccagcaaa
    36001 atgccagaga acatatacac cgtgtaaaag ctggcattga tggtcatccg acctttaaag
    36061 cgcaggcggg aaagcgcgta agccccaaga atgccgagga ataccgccac cacggatgaa
    36121 accaccgaca ccaccagact gttacggaag tagtcgacaa acgggaaaat catcgggtta
    36181 aaaatgtcga cgtaatgctc cagcgtccac tgctgcggca gcagcgtagg atgcagtgag
    36241 atcgcctctt tcgcgccctt gaacgaggtc atcagcatca caaaaaatgg aaacagcgtg
    36301 atgatgagaa acagcgccag cccgcagtaa aaaccgatgc gactgagtgt gcgtttattt
    36361 gttgccattg aggttcaccc gtttcctggt cagcagaatg acagcgaaaa taatgatgaa
    36421 gagcaccacc gagatcgccg ccgcttttcc taaatcatta aaggcaaagg cggttttgta
    36481 gagatataca ccgagaatat cgaccttggt cgtcagcaaa taaacatccg cgaacatgta
    36541 gaacatccag atggtgcgca gggtcaccac cgtcgccagg accggcataa tcgcgggcag
    36601 cgtgacgatg cgaaaccgtt gccaggcatt agcgccatcc atctctgccg cttcgtacag
    36661 cgatttgtcg atggtctgca aaatcgccag aaacgagatg aaggcatacg ggaagtagcg
    36721 ccagatggcg aacagcacca ccagcacaaa gctactgccc ggattatcga accacagcgg
    36781 cgcctgctcg tagagatgca gaagatcgac gccgaggtag ttcacaatgc cgtagccgtt
    36841 gttgaacatg tatttccagg cgaacaccag cgaaatggac ggcgttacgt aggagaggat
    36901 caccagcgaa cgcgcggttt tgcgcaggcg gaactcgcgg ttgaaaaaca tcgccacggc
    36961 cagcccgagc acggtgctgc ccgccaccac cagcgccgta taccagaccg tcatccacag
    37021 cgagtgccag aagccgggat cggagagaat gcgcacatag ttactcaccc caacaaacgt
    37081 tgactcgatg ttgggattga gcggcagacg taaaaaactg atttcgatat tcgacaccat
    37141 cggccacgcc accagaccgc ccagcagtaa taagctgggc gcgagaagca gcagcgcaaa
    37201 gggcatatcg gaacgacctg aaaaaagcct gttcattgcg gattcctgtc ctgggtttag
    37261 tgctgttcga tcagttcatc cagctttttc tggctcgctt gcagcgtgcc agggagatcg
    37321 gctttaccca cggtgacgtt atgcaccatg gagctcacca cgccagaacc cgtcacatca
    37381 cccatgcggg taaaattttt atcccctact gcgccaaaga cctgaatatt tggcagttcg
    37441 ctgattaact gatttggcag ctcacccagc gccttaataa cgtcgttgtc tttccaggtg
    37501 gcggtagtga caaccgcttt gttcaccggc agcgccgcgc ctggcgacat catcacccag
    37561 tcggcaatgt tgtctgcctg ctccataaag gtgacaaatt tttctgctgc ttccgtctct
    37621 tcggtctttt gcccggcggt aatggtcagc gaggtcaaca tgccgtagac cgcagagttt
    37681 ttctcggttg gcaccacgaa accgacgttt ttcggatcgc cttctttaat tacagccgga
    37741 aggatatagg tggagtaaat cgccagcggc gcggtgccgt tcataaaggc gtctttcact
    37801 tccatgatgt cgttagaacc cggcatcgtg ttggcggcaa ggtcgcggta ataggtcagc
    37861 gcctgcatca tctctggggt atcaagggtg attttgcctt ctgcgttaaa gacgttggcc
    37921 tggttggata acgcaaactg ggagaaggat tgttccgtca gtacgctttc tgccgtcggc
    37981 agtgcaatgc cgtatttttt attcgccggg tcattcagtt tctgtgcaac gtccagtagc
    38041 tgttgccagt ttttcggctc ctccagcccc gcttttgcca gcacatcttt gcgataccag
    38101 atgccgccaa tccaggcact cacaggaaca ccggtccatg cgctaccatc ttcggtgcgc
    38161 acaatacgca gtacgccatc gtaaaacgcg ccttcaccca cgttgctgat gactgtggca
    38221 accgctttgc gatcgataag ctgctctttg tccataactt tggcgtagtc atggctggtt
    38281 tcgatcactt ccggcagcga gccgctacgt gacagggtta tgactttggt gttataggca
    38341 tcttcttcca ctggcacttg tttaacggtg atgcccgagt tttctttttc aaaacgggca
    38401 atcaatttac tgataacggc ttgacgctcc tgctccaccg aagaatgcat aaattcgatg
    38461 gatacatggg ttttattttc ttctttacag cctgaaatca gggcgcagga aaccagtgct
    38521 gataacagca cgatttttga tttaatcatg agatgttcct tttttattta atccacataa
    38581 cctgccaggg gcgcagcgtt atttcattac ccaatatatc aacttcacta atcagatccc
    38641 gaccgtgcag atcagtaata tttatatgct gaatattttc actgacatta aacaatcctg
    38701 tcagacaatt accatccgcg ttgtttcttt gaatacgcat tacggatgaa ttaaccgtat
    38761 cgatggtaaa atcattatcc ggatgaaact cgttatgact gcaacgaagt gtaattaaac
    38821 gcgacaactg atgatatacc gcatgcctta atgtatcttc atcgttcagt tctcgggtta
    38881 tctcttcatt gtgatatttt ttacggttaa tcgcacggtt atatcccagt ttttcgacac
    38941 ctgcgtaatc attacgcgag ccaagaatac tttgaatata tatcgctggc acaccgggga
    39001 aacttaacaa aatcgcatgg gcaaggataa acctggcaca ccgctcttca tcgctactct
    39061 cgcggcggct taacgcatcc atataggtga catttatttc atagggactg cgcgtaccgt
    39121 cgggattatt tttccagttt actaatgcac cttcctgctg taacgcctcg accagcgcta
    39181 atatttcgct ttctggtaac aagccacgca gtgggttcag cccgatgcca tcgtgagagg
    39241 cgaggaagtt aaaccaggtg gtgttgctgg aaggtagtgt caggttttgc gcccacgcac
    39301 aaagcgcctc aacgttctgt ttttgcaccg catgcagcac cagcggcggc agcgagaact
    39361 ggtacaccat atgcgcttcg tcatcgcctt cgccaaagta agcaatgttg tctttatgcg
    39421 gaacattggt ctcggtaatg atcactgtac ctggtgctac gtcatcaata atcgaccgta
    39481 acagcttgat aatcagatgt gttttttcca gatggatgca gcttgttcct ggctctttcc
    39541 acataaagcc aacggcatcc agccggacat attctgcgcc cttttccagg tagcacagca
    39601 atacatccac catcgccagc aacacttcag ggctacggta attgaggtca atttgatcgt
    39661 cgctaaaggt ggtccataaa tggcgcgtgg aattatcatc catttgaaac ggtgttaata
    39721 gcggtaacgc acgcggacgg gtgacggcgc tgagatcggt ttgcggatca acagcaataa
    39781 aaaaatcttc aaaacctggc tgctgttgta aatagttttt aaaccattca ctttttgccg
    39841 acatatggtt gcagacaaaa tcaaacatta aatgactgca ttcaccgagt tgctgaatat
    39901 cctgccactc ccccgcttca ctggcgacct gatgataatc aattaccgaa aacccatcat
    39961 cagatgacca tggataaaaa ggtaataaat gaacatgtga aaaaatgctt tgcagccatt
    40021 gatggtaaaa ctgattaaat gtgggtagtg gttttaaatc attactgtga aattgatcag
    40081 cataggtaat taacacgaca tcactttcat cccagtgttt tttacgtcgc tgtgtaatta
    40141 atcgtttcgc actctcaaga cgcgttacaa gtttctgtaa atgagtcgcg gtaaatgttc
    40201 caccgtagat ttcgtccagg taatccgtaa ttttctgttt catgttgcct ttccgtggta
    40261 gcggtcccat gaaacgagag actactccgc agaaaataaa ctgtcaattg agcacaaggg
    40321 ttttagtcac gcaggaaatc attttttacc agggaaaaag cgtatttgtg agctgacgca
    40381 aataatctat ggggatgtaa ataaggtgtg acagaaaaag taaacggcgc ataagcgccg
    40441 cttagggtgg attcttttaa cctttgacct tcggcattgc gatgtctttc aggccaccgg
    40501 cgttctcaac gtgggtatat cccatctcgc taaggatctc ttttgcctgc cctgactggc
    40561 gtccggcatt gcaatacact ttcacggtgt cgtttttatc cggaacggcg gtggcaatgc
    40621 gctcttttac ttccttcagg ggaatattga tggctccctg aacgtgctct tgctgatact
    40681 gctctggaac acgaacatcg atccaatgtt cagcggcgaa aacgggcagt gaaaacacca
    40741 gtgtcagagc aagtaagcct tttttaaaca tgtaagcgtc cttctatgga tgaaaattgc
    40801 catccataat atgccttagc tttaacaatt tttgcgaaac tcccctcacc ttttataacg
    40861 ctgcgccagt ttattggcag cccgacttaa cagcggttcc agcgccacgg ccagcaacat
    40921 tttcagtggt cggcgagcaa ctgattttat cgcccagcca gctacacccg ccgggccata
    40981 gcgcagtgcg gtaagaagta ccagcttgcc tgctaatttg aaaccaggct tcaccttttg
    41041 cccggcctgt tgccagcgag tattcataac tttcctcaca gttgacggaa acggctacgt
    41101 aacgtgaaag tatcggaagt gacataacgt tccatctcgc gtaaacgcgt ttcacttgcc
    41161 gccagttcac ggtcgacttc atccagcaat tcactgctgg aaggtagctg ctcaccaaag
    41221 gccatgttgt ccggcattgg atcaagcgca aatgacaaaa tgatgtaagc aaccagggta
    41281 aacagcgcca gaccgaagaa aatcgacagc accaccagga tacgcaccag ttttactggt
    41341 acatcaaaat agttggcaat cccggcgcag acgccgcgta ccatgccctg ctgcggaata
    41401 cgccataatt ttttattgag attaatgccc gccattagcg atccctccag ttcggatgtt
    41461 ctgcatcaag aatagattcc agcgcctgaa tacgttcgcg catccgtttt gcttcatcag
    41521 ccagttgcgc taatcgctgc tgctcacttt gcgacaattc actgcgacca gaacgattgc
    41581 tgtaatgcag ccataaccag atcggtaaaa caaacagcac aaaaatggtt aacggaatag
    41641 ccagaaatag cgcgctcata aggactcctt agacgatgag cggcggcgcg ttcggacgcc
    41701 gcctggatgt tattattgat tgtcttgctt cattttggct tttaattgtg ccagttgctc
    41761 gctgattgca tcatcggctt tcagttcggc aaactgatcg tccagcgatt tttgtttacc
    41821 gaagctgtgg ctttctgctt ccgcttccat ctggtcaatt cgacgttcga aagattcgaa
    41881 acgagccatt gcttcatcca gtttgccact gtccagctga cgacgcacat cgcgcgacga
    41941 gtttgccgcc tgatggcgta acatcaatgc ctgctggcga gcgcgtgttt cgctcaattt
    42001 gttttccagc tcgccgatct ctttcttcat gcgtgccagc gtatcgtcca ccagcgtcac
    42061 ttcatgttcc aggctcttaa tcagatcggt cagtttctgt ttttcaatta acgccgcacg
    42121 cgccagatcc tctctctctt tcagcagcgc cagttcggct ttttcctgcc attcaacttc
    42181 acgcgccgac gcttgttcaa tacggcgagt cagctgtttc ttttctgcca gcgcacgcgc
    42241 cgaagtagaa cgtacttcaa ccagtgtatc ttccatctcc tggatcatca gacgcaccag
    42301 tttctgtgga tcttccgctt tctctaacag agcgttgatg ttggcattca cgatgtcggc
    42361 aaagcgagaa aaaataccca taatgttgtc ctcttgattt ctgcgttcag gattgtcctg
    42421 ctgaactatt aatacaattt gcgtgccaat tttttatctt tttgatttat aatgatctga
    42481 ttgaagaatc aacagcaaca tgccaggatg agttagcgaa ttacactaac aagtggcgaa
    42541 tttcatcatg gcagaataca aagataattt acttggtgag gcaaacagct ttctcgaagt
    42601 gctggaacag gtttcgcatc tcgcaccgct ggacaaaccg gtgctcatca tcggcgagcg
    42661 cggcacaggt aaagagctga ttgccagccg tctgcattat ctctcctccc gttggcaggg
    42721 gccgtttatt tcccttaact gcgcggcgtt aaatgaaaat ctgctggatt ccgaactgtt
    42781 tggtcacgaa gcgggcgcgt ttaccggtgc gcaaaaacgt catccaggga gatttgaacg
    42841 tgccgacggc ggtacgctat ttcttgatga actcgctacg gcaccgatga tggtgcagga
    42901 gaaattattg cgcgtgattg agtacggtga actggagcgc gttggcggca gccagccatt
    42961 gcaggtgaat gtgcggttgg tatgcgcgac gaatgccgat ctcccggcga tggtcaatga
    43021 aggtactttt cgcgctgacc tgctcgaccg actggctttt gatgttgtac aactgccgcc
    43081 cctgcgcgag cgcgaaagcg acattatgct gatggcagaa cactttgcca ttcagatgtg
    43141 tcgggaaatc aagctgcctc tgttcccggg gtttacggag cgcgccagag aaacattgct
    43201 gaattatcgc tggccgggaa atattcgtga attgaaaaac gtggtggaac gttcagtgta
    43261 tcgccacggc accagcgatt atccgcttga tgacatcatt attgatccct ttaaacggcg
    43321 tccgcctgaa gacgctatcg ccgtttcaga aaccacctcg cttccaacac tgccgctgga
    43381 tttacgtgag tttcagatgc agcaggaaaa agagttgctg caactcagtt tgcaacaagg
    43441 gaaatataac cagaagcgcg cggctgaatt actggggtta acctatcatc agttccgcgc
    43501 gttgttgaaa aagcaccaga tttagcgaag ttgggcaata caacttgccg gatgcggcgt
    43561 gaa
//
LOCUS       DAPOZO010000036        42936 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPOZO010000036 DAPOZO010000000
VERSION     DAPOZO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.036"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(41..286)
                     /gene="zapB"
                     /locus_tag="ROI21_002087"
     CDS             complement(41..286)
                     /gene="zapB"
                     /locus_tag="ROI21_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDX5111007.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            711..1556
                     /gene="glpF"
                     /locus_tag="ROI21_002088"
     CDS             711..1556
                     /gene="glpF"
                     /locus_tag="ROI21_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDX5111008.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            1579..3087
                     /gene="glpK"
                     /locus_tag="ROI21_002089"
     CDS             1579..3087
                     /gene="glpK"
                     /locus_tag="ROI21_002089"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDX5111009.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            3222..4232
                     /gene="glpX"
                     /locus_tag="ROI21_002090"
     CDS             3222..4232
                     /gene="glpX"
                     /locus_tag="ROI21_002090"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDX5111010.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            4329..5075
                     /gene="fpr"
                     /locus_tag="ROI21_002091"
     CDS             4329..5075
                     /gene="fpr"
                     /locus_tag="ROI21_002091"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDX5111011.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(5080..5508)
                     /gene="uspD"
                     /locus_tag="ROI21_002092"
     CDS             complement(5080..5508)
                     /gene="uspD"
                     /locus_tag="ROI21_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDX5111012.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(5535..5834)
                     /locus_tag="ROI21_002093"
     CDS             complement(5535..5834)
                     /locus_tag="ROI21_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDX5111013.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(6046..6486)
                     /locus_tag="ROI21_002094"
     CDS             complement(6046..6486)
                     /locus_tag="ROI21_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDX5111014.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTEDVKYKADNKSSN"
     gene            6587..7186
                     /locus_tag="ROI21_002095"
     CDS             6587..7186
                     /locus_tag="ROI21_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDX5111015.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            7294..8061
                     /gene="tpiA"
                     /locus_tag="ROI21_002096"
     CDS             7294..8061
                     /gene="tpiA"
                     /locus_tag="ROI21_002096"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDX5111016.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(8116..8871)
                     /gene="cdh"
                     /locus_tag="ROI21_002097"
     CDS             complement(8116..8871)
                     /gene="cdh"
                     /locus_tag="ROI21_002097"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDX5111017.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFNIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(8978..9967)
                     /gene="sbp"
                     /locus_tag="ROI21_002098"
     CDS             complement(8978..9967)
                     /gene="sbp"
                     /locus_tag="ROI21_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDX5111018.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(10287..11249)
                     /gene="pfkA"
                     /locus_tag="ROI21_002099"
     CDS             complement(10287..11249)
                     /gene="pfkA"
                     /locus_tag="ROI21_002099"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDX5111019.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(11430..12332)
                     /gene="fieF"
                     /locus_tag="ROI21_002100"
     CDS             complement(11430..12332)
                     /gene="fieF"
                     /locus_tag="ROI21_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDX5111020.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(12481..12981)
                     /gene="cpxP"
                     /locus_tag="ROI21_002101"
     CDS             complement(12481..12981)
                     /gene="cpxP"
                     /locus_tag="ROI21_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDX5111021.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTADNFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            13131..13829
                     /gene="cpxR"
                     /locus_tag="ROI21_002102"
     CDS             13131..13829
                     /gene="cpxR"
                     /locus_tag="ROI21_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDX5111022.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            13826..15199
                     /gene="cpxA"
                     /locus_tag="ROI21_002103"
     CDS             13826..15199
                     /gene="cpxA"
                     /locus_tag="ROI21_002103"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDX5111023.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETLKANQLWGEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(15305..15979)
                     /gene="yiiM"
                     /locus_tag="ROI21_002104"
     CDS             complement(15305..15979)
                     /gene="yiiM"
                     /locus_tag="ROI21_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDX5111024.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(16128..17111)
                     /gene="kdgT"
                     /locus_tag="ROI21_002105"
     CDS             complement(16128..17111)
                     /gene="kdgT"
                     /locus_tag="ROI21_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDX5111025.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPEAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(17371..17991)
                     /gene="sodA"
                     /locus_tag="ROI21_002106"
     CDS             complement(17371..17991)
                     /gene="sodA"
                     /locus_tag="ROI21_002106"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDX5111026.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            18276..19310
                     /gene="rhaT"
                     /locus_tag="ROI21_002107"
     CDS             18276..19310
                     /gene="rhaT"
                     /locus_tag="ROI21_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDX5111027.1"
                     /translation="MINAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFTRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(19307..20173)
                     /gene="rhaR"
                     /locus_tag="ROI21_002108"
     CDS             complement(19307..20173)
                     /gene="rhaR"
                     /locus_tag="ROI21_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDX5111028.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASARQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDEFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(20229..21065)
                     /gene="rhaS"
                     /locus_tag="ROI21_002109"
     CDS             complement(20229..21065)
                     /gene="rhaS"
                     /locus_tag="ROI21_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDX5111029.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQKTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            21353..22822
                     /gene="rhaB"
                     /locus_tag="ROI21_002110"
     CDS             21353..22822
                     /gene="rhaB"
                     /locus_tag="ROI21_002110"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDX5111030.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRNIYQRSGIQFLPFNTLYQLHALTEQQPELIPHIAHALLM
                     PDYFSYRLTGEMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            22819..24078
                     /gene="rhaA"
                     /locus_tag="ROI21_002111"
     CDS             22819..24078
                     /gene="rhaA"
                     /locus_tag="ROI21_002111"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDX5111031.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYSARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLKSVRAYEKEILSQRG"
     gene            24161..24985
                     /gene="rhaD"
                     /locus_tag="ROI21_002112"
     CDS             24161..24985
                     /gene="rhaD"
                     /locus_tag="ROI21_002112"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDX5111032.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGTGY
                     HILWGLTHEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            24995..25309
                     /gene="rhaM"
                     /locus_tag="ROI21_002113"
     CDS             24995..25309
                     /gene="rhaM"
                     /locus_tag="ROI21_002113"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDX5111033.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(25350..26744)
                     /locus_tag="ROI21_002114"
     CDS             complement(25350..26744)
                     /locus_tag="ROI21_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDX5111034.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            27221..27667
                     /locus_tag="ROI21_002115"
     CDS             27221..27667
                     /locus_tag="ROI21_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDX5111035.1"
                     /translation="MAALTASCIDLNIQGNGTYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIDQLRQGDADQVLALLNQTLSS"
     gene            27678..29129
                     /locus_tag="ROI21_002116"
     CDS             27678..29129
                     /locus_tag="ROI21_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDX5111036.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVIFATGRGLSGDDRARFAGKMVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAASLATFIAPKLFTQQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            29119..30189
                     /locus_tag="ROI21_002117"
     CDS             29119..30189
                     /locus_tag="ROI21_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDX5111037.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            30189..31937
                     /locus_tag="ROI21_002118"
     CDS             30189..31937
                     /locus_tag="ROI21_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDX5111038.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGNAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERGFCLASRPGLGHFIDETEEKRIILLANLLRKDPFLIPLA
                     GVTRDNLQQLSTVCDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGLFLGDNAVRTLTGLIEKQHQQAQVICVDNVLALLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIGEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNCIIVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVTQHHITADEVQRIIARESEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVVNHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(31993..32634)
                     /locus_tag="ROI21_002119"
                     /pseudo
     CDS             complement(31993..32634)
                     /locus_tag="ROI21_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
     gene            complement(32723..33556)
                     /gene="fdhD"
                     /locus_tag="ROI21_002120"
     CDS             complement(32723..33556)
                     /gene="fdhD"
                     /locus_tag="ROI21_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDX5111039.1"
                     /translation="MKKTQQKEIENVTNVTGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            33750..36800
                     /gene="fdnG"
                     /locus_tag="ROI21_002121"
     CDS             33750..36800
                     /gene="fdnG"
                     /locus_tag="ROI21_002121"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:34335..34337,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDX5111040.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            36813..37715
                     /gene="fdoH"
                     /locus_tag="ROI21_002122"
     CDS             36813..37715
                     /gene="fdoH"
                     /locus_tag="ROI21_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDX5111041.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            37712..38347
                     /gene="fdoI"
                     /locus_tag="ROI21_002123"
     CDS             37712..38347
                     /gene="fdoI"
                     /locus_tag="ROI21_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDX5111042.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            38344..39273
                     /gene="fdhE"
                     /locus_tag="ROI21_002124"
     CDS             38344..39273
                     /gene="fdhE"
                     /locus_tag="ROI21_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDX5111043.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(39455..39697)
                     /locus_tag="ROI21_002125"
     CDS             complement(39455..39697)
                     /locus_tag="ROI21_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111044.1"
                     /translation="MNSLAGIDMGRILLNLSNELFKQLDDLEVQHNLPRAELLREAVD
                     QYLVNQSQTARASAFGIWQGSEEDGVQYQRKLREEW"
     gene            complement(39916..40134)
                     /locus_tag="ROI21_002126"
     CDS             complement(39916..40134)
                     /locus_tag="ROI21_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDX5111045.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(40553..40831)
                     /locus_tag="ROI21_002127"
     CDS             complement(40553..40831)
                     /locus_tag="ROI21_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111046.1"
                     /translation="MSTLNTRKDRDRNGKAKPHNHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(41190..41480)
                     /locus_tag="ROI21_002128"
     CDS             complement(41190..41480)
                     /locus_tag="ROI21_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5111047.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRAS
                     GLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(41481..41792)
                     /locus_tag="ROI21_002129"
     CDS             complement(41481..41792)
                     /locus_tag="ROI21_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDX5111048.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            42021..42929
                     /locus_tag="ROI21_002130"
     CDS             42021..42929
                     /locus_tag="ROI21_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX5111049.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMILRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEQTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
BASE COUNT        10593 a        11082 c        10784 g        10477 t
ORIGIN      
        1 gcctacacag taaagaaatt acgcggaaga tgaagcgtaa tcagacctct tccatgcgac
       61 ccagcagggc ctgcagacgt tcctgccagc cgttctgctg ttctttcaga tggttgttct
      121 cacgctccag ctcttcgcgc tgatgctggg cattttgaac ttcctgcgac agtgagttgt
      181 ttttttcttt cagctcttcg atttccatct gcaacagagt gatggtatca atcgcctgct
      241 gtacttttgc ttccagtttc tcaaacactt ctaatgacat tgtcatacct ctcctgaatt
      301 gcaaggcgtt gatggataaa aatcctcgtc ccgattaccg gtgacgcctt aataaatacg
      361 agcgcacttt agttagctcc gattgtatga agccgcgcca tcgctgtcca gcggcacgcc
      421 ttgcagatta cggtttgcca cacttttcat ccttctcctg gtgacataat ccacatcaat
      481 cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa acatgcatca tgtacaatca
      541 gatggaataa atggcgcgat aacgctcatt ttatgacgaa gcacacacat tttaagttcg
      601 atatttctcg tttttgctcg ttaacgataa gtttacagca tgcctacaag catcgtggag
      661 gtccgtgact ttcacgcata caacaaacat taactcttca ggatccgatt atgagtcaaa
      721 catcaacctt gaaaggccag tgcattgctg aattcctcgg taccgggttg ttgatttttt
      781 tcggggtggg ttgcgttgca gcactaaaag tcgctggtgc gtcttttggt cagtgggaaa
      841 tcagtgtcat ttggggactg ggggtggcaa tggctatcta cctgaccgca ggggtttccg
      901 gcgcgcatct taaccccgct gttaccattg cattgtggct gtttgcctgt ttcgacaagc
      961 gcaaagttat tccttttatc gtttcacaag ttgccggcgc tttctgcgct gcggctttag
     1021 tttacgggct ttactacaat ttatttttcg acttcgaaca gactcatcac attgttcgcg
     1081 gcagcgttga aagtgttgat ctggctggca ctttctctac ttacccgaat cctcatatca
     1141 attttgtgca ggctttcgca gttgagatgg tgattaccgc tattctgatg gggctgatcc
     1201 tggcgttaac ggatgatggc aacggtgtac cacgcggccc tttggctccc ttgctgattg
     1261 gtctactgat tgcggtcatt ggcgcatcta tgggcccatt gacgggtttt gccatgaacc
     1321 cagcgcgtga cttcggtccg aaagtctttg cctggctggc gggctggggc aatgtcgcct
     1381 ttaccggcgg cagagacatt ccttacttcc tggtgccgct ttttggccct atcgttggcg
     1441 cgattgtagg tgcatttgcc tatcgcaaac tgattggtcg ccatttgcct tgcgatatct
     1501 gtgttgtgga agaaaaggag accacaactc cttcagaaca aaaagcttcg ctgtaatatg
     1561 actacgggac aattaaacat gactgaaaaa aaatatatcg ttgcgctcga ccagggcacc
     1621 accagctccc gcgcggtcgt aatggatcac gatgccaata tcattagcgt gtcgcagcgc
     1681 gaatttgagc aaatctaccc aaaaccaggc tgggtagaac acgacccaat ggaaatctgg
     1741 gccacccaaa gctccacgct ggtagaagtg ctggcgaaag ccgatatcag ttccgatcaa
     1801 attgcagcta tcggtattac gaaccagcgt gaaaccacta ttgtctggga aaaagaaacc
     1861 ggcaagccta tctataacgc cattgtctgg cagtgccgtc gtaccgcaga aatctgcgag
     1921 catttaaaac gtgacggttt agaagattat atccgcagca ataccggtct ggtgattgac
     1981 ccgtacttct ctggcaccaa agtgaagtgg atcctcgacc atgtggaagg ctctcgcgag
     2041 cgtgcgcgtc gtggtgaatt gctgtttggt acggttgata cgtggcttat ctggaaaatg
     2101 actcagggcc gtgtccatgt gaccgattac accaacgcct ctcgtaccat gttattcaac
     2161 atccataccc tggactggga cgacaaaatg ctggaggtgc tggatattcc gcgcgagatg
     2221 ctgccagaag tgcgtcgttc ttccgaagtg tacggtcaga ctaacattgg cggcaaaggc
     2281 ggcacgcgta ttccaatctc cgggatcgcc ggtgaccagc aggcggcgct gtttggtcag
     2341 ttgtgcgtga aagaagggat ggcgaagaat acctacggca ctggctgctt tatgctgatg
     2401 aacaccggcg agaaagcggt gaaatcagaa aacggcctgc tgaccaccat cgcctgtggc
     2461 ccaactggcg aagtaaacta tgcgctggaa ggtgcggtgt ttatggcggg cgcatccatt
     2521 cagtggctgc gcgatgagat gaagttgatt aacgacgcct acgattccga atactttgcc
     2581 acgaaagtgc aaaacaccaa cggtgtgtat gtggtgccgg catttaccgg gctgggtgcg
     2641 ccgtactggg atccgtatgc gcgcggggcg attttcggtc tgactcgtgg ggtgaacgct
     2701 aaccacatta tacgcgcgac gctggagtct attgcttatc agacgcgtga cgtactggaa
     2761 gcaatgcagg ccgactccgg tattcgtctg cacgcgctgc gcgtggatgg cggtgcagta
     2821 gcaaacaatt tcctgatgca gttccagtcc gatattctcg gtacccgcgt tgagcgcccg
     2881 gaagtgcgcg aagttaccgc attgggtgcg gcctatcttg ctggtctggc ggttggcttc
     2941 tggcagaatc tcgacgagct gcaagagaaa gcggtgattg agcgcgagtt ccgtccaggc
     3001 atcgaaacca ctgagcgtaa ttaccgttac gcaggctgga aaaaagcggt gaaacgcgcg
     3061 atggcgtggg aagaacacga cgagtaatgt aaataccgga tgaagcgttt atgccgcatc
     3121 cggtagtcct gaatcgtgcg ggggcaaccc cgcacacatc aataatcccg cccttcccct
     3181 gtgctacact tcgcgccatt ccttactgct tagagtttgc tatgagacga gaacttgcca
     3241 tcgaattttc ccgcgtcacc gaatctgcgg cgctggctgg ctacaaatgg ttaggacgcg
     3301 gcgataaaaa caccgcggac ggcgcagcgg taaacgccat gcgtattatg ctcaaccagg
     3361 tcaacattga cggcaccatc gttattggtg aaggtgaaat cgacgaagca ccgatgctct
     3421 acattggtga aaaagtcggt actggtcgcg gcgacgcggt agatattgct gttgatccga
     3481 ttgaaggcac gcgcatgacg gcgatgggcc aggctaacgc gctggcggtg ctggcagtag
     3541 gcgataaagg ctgtttcctc aacgcgccag atatgtatat ggagaagctg atcgtcggac
     3601 cgggagccaa aggcaccatt gatctgaacc tgccgctggc ggataacctg cgcaatgtag
     3661 cggcggcgct cggcaaaccg ttgagcgaac tgacggtaac gattctggct aaaccacgcc
     3721 acgatgccgt tatcgctgaa atgcagcaac tcggcgtacg cgtatttgct attccggatg
     3781 gcgacgttgc ggcctcaatt ctcacctgta tgccagacag cgaagttgac gtgctgtacg
     3841 gtattggtgg cgcgccggaa ggcgtggttt ctgcggcggt gatccgcgca ttagatggtg
     3901 acatgaacgg tcgtctgctg gcgcgtcatg acgtcaaagg cgacaacgaa gagaatcgtc
     3961 gcattggcga gcaggagctg gcacgctgca aagcgatggg catcgaagcc ggtaaagtat
     4021 tgcgtctggg cgatatggcg cgcagcgata acgtcatctt ctctgccacc ggtattacca
     4081 aaggcgatct gctggaaggc attagccgca aaggcaatat cgcgactacc gaaacgctgc
     4141 tgatccgcgg caagtcacgc actattcgcc gcattcagtc catccactat ctggatcgca
     4201 aagacccgga aatgcaggtg cacattctct gagtgatttg atcgattgag ccttccagtc
     4261 cttcgggact ggaatttttt tgttcggaga acgaagataa ggcaagtcaa tcaaaacagg
     4321 agaaaaacat ggctgattgg gtaacaggca aagtcactaa agtgcagaac tggaccgacg
     4381 ccctgtttag tctcaccgtt cacgcccccg tgcttccgtt taccgccggg caatttacca
     4441 agcttggcct tgaaatcgac ggtgaacgcg tccagcgcgc ctactcctat gttaactcgc
     4501 ccgataatcc cgatctggag ttttacctgg tcaccgtccc cgatggcaaa ttaagcccac
     4561 gattggcggc actgaaacca ggcgatgaag tgcaggtggt tagcgaagca gctggcttct
     4621 ttgttctgga tgaagtacca gattgcgaaa cgctatggat gctggcaacc ggtacagcga
     4681 ttggccctta tttatcgatt ctgcaactgg gcaaagattt agatcgcttc aaaaatctgg
     4741 tcctggttca cgccgcacgt tatgccgccg acttaagcta tttgccactg atgcaagaac
     4801 tggaaaaacg ctacgaaggc aaactgcgca ttcagacggt ggtcagtcgg gaaacggcag
     4861 cggggtcgct caccggacgg ataccagcgt taattgaaag tggtgaactg gaaagcgcgg
     4921 ttggcctgcc gatgaataaa gaaaccagcc atgtgatgct gtgcggcaat ccacagatgg
     4981 tgcgcgatac gcaacagttg ctgaaagaga cccggcagat gacgaaacat ttacgtcgcc
     5041 gaccgggcca tatgacagcg gagcattact ggtaagcggt tacttatcga taaacggcac
     5101 gatgagcaaa tccgcactca acttattgat catcccgcga tatgccggca tcaaacggtt
     5161 gataaatgag tggtgatgac cacagacaag gaggtcgcac tgctcttttt gcataatttc
     5221 cagcagtgtt tccggcattt ctccgcgttc aatacgcagt tttgtcttcg gccattgaat
     5281 atttttcgtc agtttataca gcttgttatc cgacttattc ttcaacaatt gaagaatatc
     5341 ttctgttgca gggaagtaga tacccggata caactcgctt aagccatcat caatatgaat
     5401 taacgtcagg tgagcgtcat tatgtctggc gagctccagg gctttattca ccagtaaggc
     5461 atcttcttca ttcccggaaa ttgccacgcc aatgtgttta taagccatgt ttaactcctt
     5521 ctaaagcaac tccatcaagc taacgaacgc agtgataact caaaaataat catctcggcc
     5581 tggcatgaga aggtgaaggc cgcatcaagc tcaacttgcc cctccagatc cttaatctta
     5641 taggccacgc tggccccttc atctgcagag gatgcagcgg cggcaagctc tttcagtttg
     5701 gtcataaagg actctgcttc ttcgcgggtg gcaaaaaagc gcgaaaactt actggtacag
     5761 ttgtcgttat caatcaccgt accgatatca atcgcacatc ctttagtact gcatttatct
     5821 acgacatctt tcatagggca cctctgtata ttcgcctctc tgtcgattac gacttaagaa
     5881 taatgcgccg ttaagggaag aacaaacaag taatgatctt atggattacc attttttttg
     5941 atattgctta aggtgaagag ttcagaagga ttttccgggt attgtcattt gcatcgtgga
     6001 aataacaatc ccggaaaaaa atagcagtaa gagatatatt aaaggttaat tacttgattt
     6061 attgtcggct ttatacttca catcctcggt atctttacca tatttatttt cgccttgtgt
     6121 gccaacaaac gcaccaagat cgataagcat catcaccaga atcaacgtcg ggacaaaacg
     6181 ccccaccgcc cattgccaga cacccggtaa aatcgcccag ttacccgcca gcagcatcca
     6241 cgccacaatc atcagaaatg cccatgcgcc ggaacgcccg cgatcatgca agcgcttaac
     6301 ggttactgcc gctgttggcc agagcaagca cacaaggcaa aacgccgcag tctgaatatc
     6361 gagtaaattt ttacccgcca gtgagaaaag caccagcatg cctgcgaacc acaggcctat
     6421 ccaaatccag aaatcacggc gtccaatacg ccctttaaat gagaataacc attgctgtat
     6481 ggtcatgtaa gttccttgat ggttgtcttt tccaggattc tacccttttg acaagggcga
     6541 cagatatcgt tttaatcgga gccagtcata acaaaaggta ctgtcaatga agccagggtg
     6601 tacgctgttt tttctcttat gttctgcatt aaccgttaca acaacggcgc atgcacaaac
     6661 accagatacg gcaacgaccg cgccttatct gctggctgga gcccctactt tcgatctctc
     6721 catcagccag tttagagaag actttaacag ccagaatccc agcctgccac tgaacgaatt
     6781 tcgtgccatc gacagcagtc ccgacaaagc caatctcact cgtgctgcca gtaaaattaa
     6841 tgagaacttg tatgcttcta cagcgctgga gcgcggtacc ttaaaaatca aaagcattca
     6901 aatgacctgg ctacccatcc aggggccaga gcaaaaagcc gcgaaagcga aagctcagga
     6961 atacatggca gcggtgatcc gcacactcac cccattaatg accaaaacac aaagccagaa
     7021 aaaactgcag tcgctactaa cggcggggaa aaacaaacgt tattacaccg agacagaagg
     7081 tgcactgcgt tatgttgtcg cggacaacgg cgaaaagggg ctgaccttcg ctgttgaacc
     7141 gattaagctg gcgctatctg aatcgcttga aggtttgaat aaatgacaaa aagcaaagcc
     7201 tttgtgccga tgaatctcta tactgtttca cagacctgct gccctgcggg gcggccatct
     7261 tcctttattc gcttataagc gtggagaatt aaaatgcgac atcctttagt gatgggtaac
     7321 tggaaactga acggcagccg ccacatggtt cacgagctgg tttctaacct gcgtaaagag
     7381 ctggcaggtg ttgctggctg tgcggttgca atcgcaccac cggaaatgta catcgatatg
     7441 gcgaagcgtg aagctgaagg cagccacatc atgctgggtg cgcaaaacgt ggacctgaac
     7501 ctgtccggcg cattcaccgg tgaaacctct gctgctatgc tgaaagacat cggcgcacag
     7561 tacatcatca tcggccactc tgaacgtcgt acttaccaca aagagtctga cgaactgatc
     7621 gcgaaaaaat tcgcggtgct gaaagagcag ggtctgactc cggttctgtg catcggtgaa
     7681 accgaagctg aaaacgaagc gggcaaaact gaagaagttt gtgcacgtca gatcgacgcg
     7741 gtactgaaaa ctcagggtgc tgcagcattc gaaggtgcgg taatcgctta cgaaccagta
     7801 tgggcaatcg gtactggcaa atctgcaact ccggcgcagg cacaggctgt tcacaaattc
     7861 atccgtgacc acatcgctaa agttgacgct aacatcgctg aacaagtgat catccagtac
     7921 ggcggctctg taaacgcgtc taacgctgca gaactgtttg ctcagccaga catcgacggc
     7981 gcgctggttg gcggtgcttc tctgaaagct gacgctttcg cagtgatcgt taaagctgca
     8041 gaagcggcta aacaggctta agtctgacag gtgccggatt tcatatccgg cacttacttt
     8101 ccttaactcc tcgccttaac gcaaaatctc acactgatga tcctggattt cctcggctga
     8161 agcacggttc agcgtcagta gatttcgttg tgtcgccagc aaaacaaacg aattatcact
     8221 ctgccgcacc atcgccagcc cgtagcttcc catatgttcc cgcgcctcag gcacttcctc
     8281 tgcaagcatc ataaatgggc tgcgttgtac cagttcgctt tccgttaccc gacgcgccag
     8341 gtactcatgc ccacgcaaac cacctggcag tggcaaccag cggctgctga tgttagccag
     8401 attgttatcc agctgttcgc gcacatcagg acgaatacaa gagatatgaa tattaaaatg
     8461 gttttgcgta cgcccggtgc gagagttgat cgccaaagaa accgcgcgat cgggaaccgg
     8521 ctggccgtat tttttgctca taaaatcacg cgcctgccag gccaaccaaa agaagttcgg
     8581 cgttgaagga tcggtcaaca aaggactttc cgtgccgtta atacgatacg ttggcatcaa
     8641 cagatattgc agtgggccat taagatcttt taaaaccacg tatccggcat tgggtttgac
     8701 ttccgcacat ggcgaaggat tttgattttg ctgttgattg ggcaaacatt cctcaaggac
     8761 aatcttacgt agtgtatccg actcttcacc ggttaatttc cagtaaccaa taccggcagc
     8821 cacaacggcg ataactatca tcaccaaaaa aagaagaccc gcttttttca tctttttttc
     8881 cctgtacctc aaagagatgc aagggtaacg caaaatcgtg acaaataaaa aacccggtcg
     8941 cgagaaacca ccgggtttga taattatcct ggggaaatca gcgtttgctg atctgatcga
     9001 acgtaccgcc gttagcaaaa tgctcttttt gcgctttcgt ccagccgccg aactcttcat
     9061 caatggtgaa taacttcagc tttggaaacg cattttcgta ctttttcgcc acctcagcat
     9121 cgcgcggacg gtagtagttt ttcgcggcaa tttcctgacc ttctggcgag tagagatatt
     9181 tcaggtaggc ttccgccacc tctttggtgc cttttttctc gaccacttta tcgaccaccg
     9241 acacggttgg ctctgcgagg atagactcac tcggcgtaac gatttcgaat ttatctttcc
     9301 ccagctcatt cgctgccagc agagcttcgt tttcccaggc aatcagtaca tcgccaattc
     9361 cgcgctcgac aaaagtgtta gtggagccgc gcgcgccaga atccagaact tcgacgtttt
     9421 tatacagtgc ccgaacaaaa tcctgtgctt ttgcctgatc gttgttgttg tgatgcagcg
     9481 cgtagcccca ggcagccagg tagttccagc gcgcgccacc agagcttttc ggattaggcg
     9541 tgatcaccga aacacccggt ttaatcagat cgttccagtc atggatctgc tttggattgc
     9601 ccttacgtac caggaaaaca atggtggaag tgtacggtgc ggagttatcc ggcagacgtt
     9661 tgatccactc tttatcaatc cgcccgcgtt ccgcaatcgc gtccacgtca taggccagag
     9721 ccagcgtgac aacatcagct tcaataccgt tgattaccga cgtcgcttgt ttacctgaac
     9781 caccgtgtga ctgacgaatc accacgttat cgccagtttg ctgtttccag tgggcgctga
     9841 atgccttgtt gtactgttcg tacaattcgc gcgttggatc atatgaaacg ttaagaagct
     9901 gaatatcctt tgccataacg ctggttgccg ccagcaaaaa tgttaaccct acgccccact
     9961 tgttcatcgc ccgactctct tatgttgtgt tgtgatgagc aaagcgtgcc agaaggttaa
    10021 ccaaacatta aagaataaaa aaagattggc tataacttgc aggtatatgt tgggggatta
    10081 aaaaggcgga agaaatccgc ctcatattgc tgacaaagtg cgctttgtcc atgccggatg
    10141 cggcgcgaac gccttatccg gcctacaaaa gtttgcaaat tcaataaatt gcagaattca
    10201 tgtaggcctg ataagcgaag cgcatcaggc atttttgctt ctgtcatcgg tttcaggcta
    10261 aaggaatctg cctttttccg aaatcattaa tacagttttt tcgcgcagtc cagccagtcg
    10321 cctttgaacg gacgcttcat gttttcgata gcgtcgatga tgtcgtggtg aaccagctgt
    10381 tcgttctgga tacctacgca acgaccgccg taacctgcca gcagcagatc gatagcgtaa
    10441 gcgcccatac gggaagccag aatacggtcg taaggcaccg gagaaccacc gcgctggatg
    10501 tggcccagca cagttgcgcg ggtttcacga ccggtttctt tctcgatgaa atgcgccagt
    10561 tcgtcaacat cacacatatg ttcggtaatc gccacgatcg cgtgtttttt acctttcgcg
    10621 atacccgctt tgatttcgtt taccaggtct tcacggctga attcaacttc cggaactaca
    10681 acgaattcac agccaccggc aatggccgca gccagggtca gatcgccaca ataacggccc
    10741 atcacttcca ccacggaaat acgctggtga gaagaagagg tgtcacgcag acggtcgatc
    10801 gcttctacaa cggtgctcag cgcagtgaag aaaccgatag tgtagtcagt gcctttgatg
    10861 tcgttgtcga tagtgcccgg cagaccgatg cacgggaagc ccatttcggt cagacgcatt
    10921 gcacccatgt aggaaccgtc accgccgata accaccagcg cgtcgatacc acgttttttc
    10981 aggttttcga tagccacggc gcggatgttc tcgtcgcgga attccgggaa acgcgcagaa
    11041 ccgaggaacg taccaccacg gttgatcatg tcagaaacgc tgtaacggtc tagctgtacc
    11101 atacggtctt catacagacc cagatagccg tcataaatgc ccattacttc cagaccttct
    11161 gtcagcgcag aacgaacaac cccgcgaatt gcggcgttca tgcctggcgc atcaccgccg
    11221 cttgtcaaca caccgatttt cttaatcatg actacctctg aactttggaa tgcaaaatga
    11281 aatctgttgc cggaagtctt cttgcacatc gaagtgatcc aacgaatgtg caaatagtat
    11341 aacaatcact tcctgctgaa ttgattcagg tcaggccaaa tggcggtatt ttatacacaa
    11401 aatgcgggtc tggctctctt ttatactgat tatgaaagca tagaccgttt accctccctg
    11461 ggtacgacgg aacaggggtc ctgatggata atcacatccg atcccggaaa acgccgtaaa
    11521 atagcctgct ctacctgatc cgccaccata tgtgcctgaa ccaaaggcag agagtcttcc
    11581 atttccaaat gaatctgaat aaagcgggtc ggccctgact gccgcgtgcg aagatcgtga
    11641 gcgccgctaa cacccggcca ggaagtcacg atatcaataa tttcttgccg ttcttcatca
    11701 ggcaatgcgc gatccagtaa tgactgtacc gcctcatatc ccatgcgtaa cgcgctatat
    11761 aaaatataga tgccgattcc caatgcaaac agagcatcgg cgcgatgcca gccgtaccag
    11821 gacaacccca gcgccagcag aattgcgccg ttcatcataa catcagactg gtaatgtagc
    11881 atatcagccc gcaccgcctg gctttgcgtc cggcgcacca cccaccgctg aaacgagaca
    11941 aggataatcg tacaaattag cgccacaatt gtcacgataa ccccgacgcc tggatctgtc
    12001 atcggtgttg gagataccag atgttgaata cccgtcaaaa agaggaatag tgccgaaccg
    12061 gagataaaca tactttgcgc cagcgccgcg agggactcag ctttaccgtg accaaacgag
    12121 tgattatcgt cggcaggttg cagggaatat cgcaccacca ataaattcgt caacgacgcg
    12181 ccgatatcca ccagcgaatc caccagcgcg gcgagaatac tcaccgaccc ggtataccac
    12241 catgcaaaaa ttttaatcag caatagcagt gaagccatcg ccgttgcagc aatcgccgcc
    12301 cgactgacca gccgtccata agattgattc ataaatactc ccgctatcaa ctgacgctag
    12361 tataacggaa gcaaatcatc tgcaatgcat taagcagcag gcaaattgag gataaaaaaa
    12421 acccccacat catgtggggg aagacaggga tggtgtctat ggcaaggaaa acagggttta
    12481 ctactgggaa cgtgagttgc tactactcaa tagcttcaac gatgaacttt tttgccattg
    12541 cgtcacgtcg cgcaactgct ccattcgttg ttgatgtttc tcgtttaaaa ccgcttgctg
    12601 ctccggcgtt aacaggcgat acatttggtt gcggactttt gccatctcaa cctgacgagc
    12661 aatttgctca ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat caaaattatc
    12721 tgcggtgaca aggcgatgca ttgtctccag ttcgctaaca ttaacaggag gctgttcgtg
    12781 ccgggcctgt tgcataagat ctcgcatctg ctgacgctga tgttcggtta aacttatgcc
    12841 gtcgaacata tggctctgcg tactgcgctg cgtaagttct tcacccggat gccagttatc
    12901 gcctgaaccg acttcagcag cgtggcttaa tgaactgact gccagcgttg aggccatgac
    12961 ggcagcggta actatgcgca tcatttgctc ccaaaatctt tctgtcgcga ttcaacgata
    13021 gagagtttac gattcaggct gcaaacatgc gtcagggggt gtaaaacaac gtaaagtcat
    13081 ggattagcga cgtctgatga cgtaatttct gcctcggagg tatttaaaca atgaataaaa
    13141 tcctgttagt tgatgatgac cgagagctga cttccctatt aaaggagctg ctcgagatgg
    13201 aaggcttcaa tgtgattgtt gcccacgatg gggaacaggc gcttgatctt ctggacgaca
    13261 gcattgattt acttttgctt gatgtaatga tgccgaagaa aaatggtatc gacacattaa
    13321 aagcacttcg ccagacacac cagacgcctg tcattatgtt gacggcgcgc ggcagtgaac
    13381 ttgatcgcgt tctcggcctt gagctgggcg cagatgacta tctcccgaaa ccgtttaatg
    13441 atcgtgagct ggtggcacgt attcgcgcga tcctgcgccg ttcgcactgg agcgagcaac
    13501 agcaaaacaa cgacaacggt tcaccgacac tggaagttga tgccttagtg ctgaatccag
    13561 gccgtcagga agccagcttc gacgggcaaa cgctggagtt aaccggtact gagtttaccc
    13621 tgctctattt gctggcacag catctgggtc aggtggtttc ccgtgaacat ttaagccagg
    13681 aagtgctggg caaacgtctg acgcctttcg accgcgctat cgatatgcac atttccaacc
    13741 tgcgtcgtaa actgccggat cgtaaagatg gtcacccgtg gtttaaaacc ttgcgtggtc
    13801 gcggctatct gatggtttct gcttcatgat aggcagctta accgcgcgca tcttcgccat
    13861 cttctggctg acgctggcgc tggtgttgat gttggttttg atgttaccca agctcgattc
    13921 acgccagatg accgagcttc tggatagcga acagcgtcag ggtctgatga ttgagcagca
    13981 tgtcgaagcg gagctggcga acgatccgcc caacgattta atgtggtggc ggcgtctgtt
    14041 ccgggcgatt gataagtggg caccgccagg acagcgtttg ttattggtga ccaccgaggg
    14101 ccgcgtgatc ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta ttggtcaggc
    14161 cgataacgcc gatcatccgc agaagaaaaa gtatggccgc gtggaactgg tcggtccgtt
    14221 ctccgtgcgt gatggcgaag ataattacca actttatctg attcgtccgg ccagcagttc
    14281 tcaatccgat ttcattaact tactgtttga ccgcccgcta ttactgctga ttgtcaccat
    14341 gttggtcagt acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac cggcgcgtaa
    14401 gctgaaaaac gctgccgatg aagttgccca gggaaactta cgccagcacc cggaactgga
    14461 agcggggcca caggaattcc ttgccgccgg tgccagtttt aaccagatgg tcaccgcgct
    14521 ggagcgcatg atgacctctc agcagcgtct actttctgat atctctcacg aactgcgcac
    14581 cccgctgacg cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg gtgaaagcaa
    14641 ggaactggag cgtattgaaa ccgaagcgca acgtctggac agcatgatta acgatctgtt
    14701 ggtgatgtca cgtaatcagc agaaaaacgc gctggtaagc gagacattga aagctaacca
    14761 gttgtggggc gaagtgctgg ataacgcggc gttcgaagca gaacagatgg gcaagtcgtt
    14821 gacagttaac ttcccgcctg ggccgtggcc gctgtacggc aacccgaacg ccctggagag
    14881 tgcgctggaa aacattgttc gtaatgccct gcgttattcc catacgaaga ttgaagtggg
    14941 ctttgcggta gataaagacg gtatcaccat tacggtggac gacgatggtc ctggcgttag
    15001 cccggaagat cgcgaacaga ttttccgtcc gttctatcgt accgatgaag cacgcgatcg
    15061 tgaatctggc ggtacaggtt tggggctggc gattgttgaa accgccattc agcagcatcg
    15121 tggctgggtt aaagcggaag acagcccgct gggcggttta cggctggtga tttggttgcc
    15181 gctgtataag cggagttaaa ctccgcattt gtaggcagga taaggcgttt acgccgcatc
    15241 cggcatttga gcaggatgcc tgatgcgacg ctgatagcgt cttatcaggc ctacactccg
    15301 ggggttattt tccccacaac cggcgggaaa aatcttcgat cttgccgctc agtcggcgct
    15361 tttgcatcgt ccgcgtccag cttttcgata acccggcggc ggagagtaaa cggtgatact
    15421 gatcgtcatc aaacggcata tgccatgcga tggcagcagc ctcctgcacg gtcacatcgc
    15481 tgacacggga aaccaactcc agtggcgcat cagcagatac caaccctggc gcaatcacgc
    15541 tatacaacca cccaacttta cccgtgtttt gcatcaactg cgctatatcg ctgatatcaa
    15601 aatggtaatt gagtttatag caaggtgagc gtggctggct gacctgaatt aatgcctctc
    15661 cccagcggaa aatatcgcca atataaacat tactttccgt cagaccgtcg gttgagagat
    15721 tttcaccaaa cgcaggcgca acaaacaact ccgcctgttc cggaaattcc cgcgcccagt
    15781 agagatagtg ctcacgagga taatgacaca gcgctctgtc tggcccgccg tgaaccttct
    15841 tctccgcctg ctcgtcacct tccagcccca gctccgtcag catcaactca ccatcaacct
    15901 ggattttagc aattgcgctg ggtttacctt cgggataagc ctgaatcttg cctgtgtata
    15961 catcaaccgg atatcgcatc tgttccctct ccacgagaaa tttccccatt agaccatatt
    16021 tattttatgt caggcgtgct gttttcgcgg taagtatcca ggcaagaact caatagcaaa
    16081 ccggacgaac gttagttcat ccggctgtgg ggattaagga agcaggttta tttcaccgta
    16141 cctaaaattt cgattttcgc tgctctggct ttgactttac gtgaccagat agaggtaaga
    16201 attggcacca gaatcgaagt cacaatgacc gccgtcgcga ccagcgaagt tgctgccgga
    16261 gccatcggtt taaacgcagg caccatttct gcaatcagca caggtgtcgc taccgcggcc
    16321 cctgcggaac tggaagcggc aattccggca gtgccatcgc caccgccaat caatttatcg
    16381 gcgataatca acggaatacc ggtcacgata attactgcca cacccaacag gatccccagc
    16441 aaacctgtct gggcaattac agtcaaatca atggtattgc ccagcgcgaa ggcaaagaat
    16501 ggaatcagcg tctgcaccgc tttgctgaaa aattctcgta attcagggtc aaggttccca
    16561 agggcaaagc ccaccaggaa cggtaatact gcgccgacga aaacatgcgg ttcaaacgag
    16621 gcaatcccgg cagtgcccag aataatcatc gtcatgagcg gcccggactc caatgacatc
    16681 aacacaaatg ccccagcttc ttcttttgtg ccgtactgct gcatgatgga agcgtaaagt
    16741 ccgccgttgg tcatatccat cgccgccacc agcgccagcg ttgaaagtcc ggcaaagaat
    16801 ccaacttcaa caccatgttc cggaataatg cgcgaggcaa ttgccgcaac cacccacgcg
    16861 acggcaattt tagttaccac cagcgtaccg gatttacgca gtaccgttcc cgtcgcgctt
    16921 aattttattg acgcccccat gcaaaaaaac cacaccgcca gaatgggcac cgtaccggta
    16981 atcatcccgt tggtgaatga tccaaaatat ttccccgcct ccggcgagaa ggtgtggcac
    17041 agtgcgccaa ggaatagcgg gacgagcatc atcccccccg ggattttctc aatcgagcgt
    17101 tttatctgca tttccatcac ctgtcggcat atttttaagg gctgatgcgt cattcctcaa
    17161 tggagcatca gtgttgatgg aaacgaagat agacattcat ttaatcaata aaagtgatca
    17221 aaaccacatc aattgaaaca ctgttttatt tttatcggat cattgttttt attttgaaat
    17281 tgatcacaaa aaaaccaccg ccgttggcga tggttcattt tttaagctga tatgcggcgt
    17341 aaacgcctca ttgcagcagg cggcaaatga ttattttttc gccgcgaaac gtgccgctgc
    17401 ttcgtcccag ttcaccacgt tccagaactc tttgatgtag tcaggacggc ggttctggaa
    17461 tttcaggtag taagcatgtt cccacacatc caggcccaga atcgggaagc cggaagcgcc
    17521 agaaatagct tcacccatca gcggggaatc ctggttagca gtagaaacca ccgccagttt
    17581 atcgcctttc agcaccagcc atgcccagcc ggaaccaaag cgggaagctg ccgctttctc
    17641 aaattctgct ttgaagttat caacggagcc gaagtcacgt tcgatagccg ctttcaggtc
    17701 accctgcagg gtggtgcctt ttttcagacc tttccagaac aggctgtggt tagcgtgacc
    17761 gccagcgttg ttgcgcagta cggttttctt gtctgctggc agctggtcca gtttggtgat
    17821 cagctcttca accggcaggt tggcaaattc tggcaggctt tccagcgccg cgttggcgtt
    17881 gttgacgtag gtctgatggt gtttggtgtg gtggatttcc atggtctgct tatcgaagtg
    17941 cggttccagg gcatcgtaag catacggcag ggatggcagg gtataactca tattcatctc
    18001 cagtgttgtc gggcggccga ttgttaatgc cgcgtaagca gttggttcat tatagttaat
    18061 taaatgatat tgaaaatgat tatcaatgcc gtacttttcg taagggtatg gttttgcagg
    18121 aaaatgcccg agatgtgaag caaatcaccc acttaatgcc gtgattgcca gtaaatcgac
    18181 aacggcggca acaggcgaaa ggttaatcga cagcacgatt tttacactca tctcgtcgga
    18241 gatgtgacgc gacgaaaaat gatgaggata agaagatgat taacgcgatt acgatgggga
    18301 tattttggca tttgatcggc gcggccagtg cagcctgttt ttacgctccg ttcaaaaaag
    18361 taaaaaaatg gtcatgggaa accatgtggt cagttggtgg gattgtttcg tggattattc
    18421 tgccgtgggc catcagcgcc ctgttactac cgaatttctg ggcgtattac agctcgttta
    18481 gtctctctac gctgctgcct gtttttctgt tcggcgcaat gtgggggatc ggtaatatca
    18541 actacggcct gaccatgcgt tatctcggca tgtcgatggg aattggcatc gccattggca
    18601 ttacgttgat tgtcggtacg ctgatgacgc caattatcaa cggcaatttc gatgttctga
    18661 ttaataccga aggcggacgc atgacgttgc tcggcgttct ggtggcgctg attggcgtag
    18721 ggattgtaac tcgcgccggg cagttgaaag agcgcaagat gggcattaaa gccgaagagt
    18781 tcaacctgaa aaaagggctg gtgctggcgg tgatgtgcgg cattttctct gccgggatgt
    18841 cctttgcgat gaacgccgca aaaccgatgc atgaagccgc tgctgcactg ggcgtcgatc
    18901 cactgtatgt cgctctgcca agctatgttg tcatcatggg aggcggcgcg atcatcaacc
    18961 tcggtttctg cttcactcgt ctggcaaaag tgaaggattt gtcgctaaaa gccgacttct
    19021 cgctggcaaa accgctgatc attcacaatg tgttactctc agcactgggc gggttgatgt
    19081 ggtatctgca attctttttc tatgcctggg gccacgcgcg tattccggcg cagtatgact
    19141 acatcagctg gatgctgcat atgagcttct atgtattgtg cggcggtatc gtcgggctgg
    19201 tgctgaaaga gtggaacaat gcaggtcgcc gtccggtaac ggtgttgagc ctcggttgtg
    19261 tggtgattat tgtcgccgcc aacatcgtcg gcatgggcat ggcgaattaa tctttctgcg
    19321 aattgagatg acgccactga ctgggcgtca tcccggtttc ccgggtaaac accaccgaaa
    19381 aatagttact atcttcaaag ccacattcgg ttgaaatatc actgattaac aggcggctat
    19441 gctggagaag atattgcgca tgacacactc tgacctgccg cagatattga ttgatggtca
    19501 ttccagtctg ctggcgaaat tgctgacgca aaacgcgctc actgcacgat gcctcatcac
    19561 aaaattcatc cagcgcaaag ggacttttca ggctggccgc cagccgggta atcagcttat
    19621 ccagcaacgt ttcgctggat gttggcggca acgaatcgct ggtgtaacga tggcgattca
    19681 gcaacatcac caactgcccg aacagcaact ctgccatttc gttagcaaac ggcacatgct
    19741 gactactttc atgctcaagt tgaccgataa cctgccgcgc ctgcgccatc cccacgctac
    19801 ctaagcgcca gtgtggttgc cttgcgctgg cgctaaatcc cggaatcgcc ccctgccagt
    19861 caagattcag cttcagccgc tccgggcaat aaataatatt ctgcaaaacc agatcgttaa
    19921 cggaagcgta ggagtgttta tcgtcagcat gaatgtaaaa gagatcgcca cgggtaatgc
    19981 gataagggcg atcgttgagt acatgcagac cattgccgcg ccagacaatt accagctcac
    20041 aaaaatcatg tgtatgttca gcaaagacat cttgcggata acggtcagcc acagcgactg
    20101 cctgctggtc gctggcaaaa aaatcatctt tgagaagttt taactgatgc gccaccgtgg
    20161 ctacctcggc cagagaacga agttgattat tcgcaatatg gcgtacaaat acgttgagaa
    20221 gattcgcgtt attgcagaaa gccatcccgt ccctggcgaa tatcacgcgg tgaccagtta
    20281 aactctcggc gaaaaagcgt cgaaaagtgg ttactgtcgc taaatccgca gcgataggcg
    20341 atgtcagtaa cgctggcctc gctgtggcgt agcagatgtc gggctttcat cagtcgcagg
    20401 cggttcaggt aacgctgagg cgtcagtccc gttttctgct taagctgccg atgtagcgta
    20461 cgcagcgaaa gagaaaattg atccgccacg gcatcccaat ttacctcatc ggcaaaatgg
    20521 tcctccagcc aggccagaag caagttgaga cgtgacgcgc tgttttccag gttctcctgc
    20581 aaactgcttt tacgcagcaa gagcagtaat tgcataaata agatctcgcg actggcggtc
    20641 gagggtaaat cattttcctc ttcctgctgt tccatctgtg caaccagctg tcgcacctgc
    20701 tgcaacacgc tgtggttaac gcgccagtga gacggatact gcccatccag ctcttgtggc
    20761 agcaactgat tcagcccggc gagaaactga aatcgatccg gcgagcgata cagcacattg
    20821 gtcagacaca gattatcggt atgttcatac agatgccgat catgatcgcg tacgaaacag
    20881 accgtgccac cggtgatggt atagggctgc ccattaaaca catgaatacc cgtgccatgt
    20941 tcgacaatca caatttcatg aaaatcatga tgatgttcag gaaaatccgc ctgcgggagc
    21001 cggggttcta tcgccacgga cgcgttacca gacggaaaaa aatccacact atgtaatacg
    21061 gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag taatcacgag gtcaagttct
    21121 taccttaaat tttcgacgga aaaccacgta aaaaacgtcg attattcaag atacagcgtg
    21181 aattttcagg aaatgcggtg aacatcacat caccacaatt cagcaaattg tgaacatcat
    21241 cacgttcatc tttccctggt tgccaatggc ccattttcct gtcagtaacg agaaggtcgc
    21301 gaagtcaggc gctttttaga ctggtcgtaa tgaaattcag caggatcaca ttatgacctt
    21361 tcgcaattgt gtcgccgtcg atctcggcgc atccagtggg cgcgtgatgc tggcgcgtta
    21421 cgagcgtgaa tgccgcagcc tgacgctgcg cgaaatccat cgttttaaca atgggctgca
    21481 tagccagaac ggttatgtca cctgggatgt ggatagcctg gaaagtgcca ttcgccttgg
    21541 attaaacaag gtgtgcgagg aagggattcg tatcgatagc attgggattg atacctgggg
    21601 cgtggacttt gtgctgcttg accaacaggg tcagcgcgtg ggcctgcctg ttgcttatcg
    21661 cgatagccgc accaatggcc tgatggcgca ggcacaacaa caactcggca aacgcaatat
    21721 ttatcaacgt agcggcatcc agtttctgcc cttcaatacg ctttatcagt tgcatgcact
    21781 gacagagcaa caacctgaac ttattccaca cattgctcac gctctgctga tgccggatta
    21841 cttcagttat cgcctgaccg gcgagatgaa ctgggagtac accaacgcca cgaccacgca
    21901 actggtcaat atcaatagcg acgactggga cgagtcgcta ctggcgtgga gcggtgccaa
    21961 caaagcctgg tttggtcgcc cgacgcatcc gggtaatgtc ataggtcact ggatttgccc
    22021 gcagggtaat gagattccgg tggtcgccgt tgccagccat gataccgcca gcgcggttat
    22081 cgcctcgccg ttaaacggtt cacgcgccgc ttatctctct tctggcacct ggtcattgat
    22141 gggcttcgaa agccagacgc catttaccaa tgacacggcg ctggcagcca atatcaccaa
    22201 cgaaggcggg gcggaaggtc gctatcgggt gctgaaaaat attatgggct tatggctgct
    22261 tcagcgggtg cttcaggagc ggcaaatcaa cgatcttccg gcgcttatcg ccgcgacaca
    22321 ggcactgccg gcttgccgct tcaccatcaa tcccaatgac gatcgcttta ttaatcctga
    22381 cgagatgtgc agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccga tcccggaaag
    22441 tgatgctgaa ctggcgcgct gtattttcga cagtctggcg ctgctgtatg ccgatgtgtt
    22501 gcatgaactg gcgcagctac gcggtgaaga tttctcgcaa ctgcatattg tcggcggtgg
    22561 ctgccagaac gcgctgctca accagttatg tgctgatgcc tgcggtattc gggtgatcgc
    22621 cgggcctgtt gaagcctcga cgctcggcaa tatcggcatc cagttaatga cgctggatga
    22681 actcaacaat gtggatgatt tccgtcaggt cgtcagcacc accgcgaatc tgaccacctt
    22741 tacccctaat cctgacagtg aaattgccca ctatgtggcg cagattcact ctacacgaca
    22801 gacaaaggag ctttgcgcat gaccactcaa ctggaacagg cctgggagct ggcgaaacag
    22861 cgtttcgcag cggtagggat tgatgtcgag gaggcgctgc gccaacttga tcgtttaccc
    22921 gtgtcaatgc actgctggca aggcgatgat gtttccggtt ttgaaaaccc ggaaggttcg
    22981 ctgaccgggg ggattcaggc cacaggtaat tatccgggca aagcgcgtaa tgccagcgag
    23041 ctgcgtgccg atctggaaca ggctatgcgg ctgattccgg ggccgaaacg gcttaattta
    23101 catgccatct atctggaatc agacacgcca gtctcgcgcg accagatcaa accagagcac
    23161 ttcaaaaact gggttgaatg ggcgaaagcc aatcagctcg gtctggattt taacccctcc
    23221 tgtttttcgc atccgctaag cgccgatggc tttacgcttt cccatgccga cgacagtatt
    23281 cgccagttct ggattgatca ctgcaaggcc agccgccgtg tttcagccta ttttggcgag
    23341 caacttggca caccatcggt gatgaacatc tggatcccgg atggcatgaa agatatcacc
    23401 gttgaccgtc tcgccccgcg tcagcgtctg ctggcagctc tggatgaggt gatcagcgag
    23461 aagctggatc cggcgcacca tatcgacgcc gttgagagca aattgtttgg tattggcgcg
    23521 gagagctaca cggttggctc caatgagttt tacatggggt atgccaccag ccgccagacg
    23581 gcgctgtgcc tggacgccgg gcatttccac ccgactgaag tgatttccga caagatttcc
    23641 gccgccatgc tgtatgtgcc gcagttgctg ctgcacgtca gccgtccggt gcgctgggac
    23701 agcgatcacg tggtgctgct ggatgatgaa acccaggcga ttgccagtga gattgttcgt
    23761 cacgatctgt tcgaccgggt acatatcggt ctcgatttct ttgatgcctc tatcaaccgc
    23821 attgccgcgt gggtcattgg tacacgcaat atgaaaaaag ccctgctgcg tgcgttgctg
    23881 gaacctaccg ctgagctgcg caagctggaa gcggcgggcg attacagtgc gcgtctggca
    23941 ctgctggaag agcagaaatc gttgccgtgg caggcggtgt gggaaatgta ttgtcagcgt
    24001 cacgataccc cagcaggtag cgaatggctg aagagcgtgc gggcttatga aaaagaaatt
    24061 ttgagccagc gtgggtaaac actgccggat gcggcgcgag cgccttattc ggccgacaga
    24121 tcgtgcatcc gacaacaccg aatttacagg aacaccgaac atgcaaaaca ttactcagtc
    24181 ctggtttgtc cagggaatga ttaaagccac caccgacgcc tggctgaaag gctgggacga
    24241 gcgcaacggc ggcaacctga cgctacgcct ggatgacgcc gatatcgcgc cttatcacga
    24301 caactttcat gcacaaccgc gctatatccc gctcagccag cccatgcctt tactggcaaa
    24361 tacaccgttt attgtcaccg gctccggcaa attcttccgc aacgtccagc ttgatcctgc
    24421 ggctaactta ggcgtcgtaa aagtcgacag cgacggcacg ggctaccaca ttctctgggg
    24481 gctaactcac gaagccgttc caacctctga actcccggcg cacttcctct cccactgcga
    24541 acgcattaaa gccaccaacg gtaaagatcg ggtgatcatg cactgccatg ccaccaacct
    24601 gatcgccctt acctacgtac tggaaaacga caccgcggtc ttcactcgcc aactgtggga
    24661 aggcagcacc gaatgtctgg tagtgttccc ggatggcgtc ggcattttgc cgtggatggt
    24721 gcccggcacc gacgaaatcg gccaggcgac cgcacaggaa atgcaaaaac attcgctggt
    24781 gttgtggccc ttccacggcg tcttcggcag cggtccgaca ctggatgaaa cattcggttt
    24841 aatcgacacc gcagaaaaat cagcacaagt attagtgaag gtttattcga tgggcggcat
    24901 gaaacagacc atcagtcgtg aagagctgat agcgctcggc cagcgtttcg gcgttacacc
    24961 actcgccagt gcgctggcgc tgtaaggagc aaacatgatc cgcaaagcct ttgtaatgca
    25021 ggttaacccc gacgcccacg aagagtatca gcgtcggcat aaccccatct ggccggaact
    25081 ggaagcagtg ttgaaatctc acggtgcgca taactacgcc atttacctcg acaaagcgcg
    25141 taatctgctg tttgccacgg tagagattga atctgaagaa cggtggaatg cggtagccag
    25201 cactgatgtt tgccaacgtt ggtggaaata tatgaccgac gttatgccag ctaacccgga
    25261 taacagccca gtcagtagcg agctgcaaga agtgttttac ctgccataat gagttgcggt
    25321 gccggaaaat atccagcacc gcgccttcat cagaagaagt atttaaagcg aacgcgtgcg
    25381 ccgtaacggt catcagtatc atcaccgtgt tttttatcac cgtcgctatc cagaatggag
    25441 taatacgtcc cgaggtaaat attaaagttc tccatatcca tcacattagc gaactgataa
    25501 gacgcatgaa tggtgtgaat ggtgtacgtc ccttcatcat caatccagca gtcgttatca
    25561 caaactacac cgctgaattc atcaatctta ttgtgcgcgt agatataacc tagctcgaaa
    25621 cgtttccaca gcgcgttaat cccggcagtg aaatctttct cattattagc atctaaatag
    25681 gcggtattaa gattaaccac gacgccattt tccggatccg ttttcaggcc attccaggtc
    25741 atgctcatac catagccggt acgatcggac tgatcgacaa aattaccctt gctatcggta
    25801 taaccataag cattatttac cacgttcgct tccatcgctg cggaaacggt aaattcttcc
    25861 gtcggcgacc aggcaattac cggacgcaga taagcaacat ttttctgctg ctccatatcg
    25921 cgtccatgat aattaccgtc attatataaa gatgttccgt cttcaagtaa cgtgtttaat
    25981 tcaaaatacc agttatcaag ttgtttgctg acgaggaaat taccgccagc gttagagcgc
    26041 ccgcggccct ctttcatcat atagatataa ccgctgccat cgtcataaag atcgttcgca
    26101 gtattaccgg aatgttcaac aaaggtatcc tgattcagcg ggaacatatc gtaggcttca
    26161 aaacggccta ctttgacctt ccagtcgttt tctttgccaa agaagaatac cgcatcatcg
    26221 atatttactg aaccgtgcat gtcccccagc ggttgtgcgg agaacccggc gaaatagcca
    26281 ttatccagtt ttcgcatacc atcaaaacct aacagaatac gaccattgag attccactgt
    26341 tcattagtgg gatcgctatt cacatcagcg ttggtcattg ccagcaggcc atgattactt
    26401 tccgcatcca tattaaattc tacatcgccg tagattttta aatcgccata acctgagagc
    26461 aaaagattag gtgaggtgga atttttcttt gcagattccg ccgttgcctg tccatttacc
    26521 ggcgtattgc cctgggcagc cttaatttcg cggatctcag cggcttgttg tgtctgcagc
    26581 tgtttaacct ggctctctgc ttttgctgcc cgcatttctg ccgcttccag gcgttcttcc
    26641 agcaatgcca tgcgttgttc cagcgttaaa tgctgtgttt ttgccattcc ggtactacat
    26701 gccaataata aaccgatggc taaagataat gtcgattttt tcattattta cgttccctga
    26761 aataaaagag accctacgct ttccaaaagg aatacgtata tagagaaata taaggtgtgc
    26821 actttcgaat ttttaatcca acttcagcag gcctatggat agtatcccgc tgcataatat
    26881 gagtaaatcc gctgatttcc tggaacgtga ggaatataac aatagtgccg tttttaacca
    26941 aatatcgtaa aaaatattag atataattca cgctgactca taagccacaa atataaataa
    27001 ccttgctgca aaacatgtgc ggcagttaaa cattcacaac atcattaaat aatgattttt
    27061 tattcactgc atttattttg cccatcaata tgtgccttac accgcaaaat ttatccttca
    27121 ctctgcctta tattgtcacg acttcacgac atatccgcgc tcatcacaaa accgccccgc
    27181 cgttacaatt aactataata ttttttcatg gagtccaata atggcagcac ttactgcaag
    27241 ctgtattgac ctgaatattc agggcaatgg cacttattcc gttctgaagc agttggcgac
    27301 aatggcgtta caaaacggtt ttatcaccga ctcacatcaa ttcctgcaaa ccctgctgct
    27361 gcgcgaaaaa atgcactcca cgggatttgg ttccggtgtc gccgtgccgc acggtaaaag
    27421 cgcctgcgtt aaacaaccgt tcgtgttatt cgcccgcaaa gcgcaggcca ttgactggaa
    27481 agccagcgat ggcgaagacg tcaattgctg gatctgcctc ggtgtgccgc aaagtggcga
    27541 agaggatcag gtcaaaatca tcggcacgct atgccgcaaa attatccacc aggattttat
    27601 cgaccaactg cgacagggcg acgccgacca ggtgcttgct ttgttaaatc aaaccctcag
    27661 ctcataagga cgtggcgatg gagtcatcct tacgtattgt cgcgatcacc aactgccccg
    27721 ccgggatcgc tcacacctac atggtggcgg aagccctgga acagaaagcc cgttctctcg
    27781 gccataccat aaaagtggaa actcaaggat caagcggcgt cgaaaaccgc ttatccagcg
    27841 aagagattgc cgctgccgat tacgtgattt tcgccaccgg gcgtggcctg agcggtgacg
    27901 atcgcgcgcg atttgccggg aaaatggttt atgagattgc catctcccag gcgctgaaaa
    27961 atatcgacca gattttcagt gaattaccga caaactcaca actttttgcc gcagatagcg
    28021 gcgtgaagct cggcaaacag gaagtgcaaa gtggcagcgt aatgagccat ctgatggcgg
    28081 gtgtctctgc ggcgctgccg tttgtcatcg gtggcggtat cctggtggcg cttgccaaca
    28141 tgctggtgca gttcggtttg ccgtataccg atatgtcgaa aggcgcgccg tcgtttacct
    28201 gggtggttga atccatcggt tatctcggct tcacctttat gatccccatc atgggcgctt
    28261 atattgcctc gtcgattgcc gataaacccg ctttcgctcc ggcgtttctg gtttgctatc
    28321 tggcgaatga caaagccctg ctcggcaccc agtctggcgc gggcttcctc ggcgcggtgg
    28381 tgctggggct ggcgattggc tatttcgtct tctggttccg taaagtgcgc cttggcaaag
    28441 cattacaacc gctgctcggt tccatgctga tcccgttcgt cactctgctg gttttcggcg
    28501 tactgactta ctacgttatc ggaccggtga tgtccgacct catgggcggg ttgctgcact
    28561 tcctgaacac cattcctccg tcaatgaagt ttgcggcagc gtttttggtc ggtgcgatgc
    28621 tggcgttcga tatgggcggc cctatcaata aaaccgcctg gttcttctgc ttctcactgc
    28681 tggaaaaaca catttacgac tggtacgcca tcgtcggcgt tgtcgcgctg atgccgcctg
    28741 tcgcggcgag tcttgcgacg ttcattgcgc ccaaactgtt tactcaacag gaaaaagaag
    28801 ccgccagtag cgccattgtg gtcggtgcca cggtcgcgac cgaaccggct attccttacg
    28861 ctctggcggc accactgcca atgattaccg ccaatacgct ggcgggcggc attaccggcg
    28921 tgctggtgat cgctttcgga atcaaacgac tggcacctgg tttgggtatc tttgacccgc
    28981 tgatcggcct gatgtcgccg gtgggttcgt tttatctggt gctggcgatt ggcctcgcgc
    29041 tgaacatttc attcattatc gtcctgaaag gattgtggtt acgccgcaaa gcgaaagccg
    29101 cgcaacagga gcttgtccat gaacattgag ttactgcaac agttgtgcga agccagcgcc
    29161 gtcagcggcg atgaacagga agttcgcgac attctgataa acacgctgga accttgcgtt
    29221 aatgagatca cctttgatgg tctgggcagc tttgttgccc gtaagggaaa taaaggtcca
    29281 aaagttgccg ttgtcgggca tatggatgaa gtcggcttta tggtcaccca catcgacgag
    29341 agcggttttc tgcgttttac caccattggc ggctggtgga atcagtcgat gctcaaccac
    29401 cgggtaacca tacgcacaca caagggattt aaaatccctg gtgtgattgg ttccgtcgcg
    29461 cctcatgcgt taacggaaaa gcaaaagcaa caaccgctgt catttgatga gatgttcatt
    29521 gatattggcg cgaacagtcg cgaagaagca gaaaaacgcg gcgtggaaat tggcaatttt
    29581 attagcccgg aagccaattt tgcctgctgg ggcgaagata aagtggtcgg caaggcgctg
    29641 gataatcgca tcggctgcgc aatgatggct gagctattgc agacggtgaa taaccccgaa
    29701 attacgctgt atggcgttgg cagtgtggaa gaagaagttg ggctacgcgg ggcgcaaacc
    29761 tcggctgaac acattaaacc ggatgtggtg atcgtgctgg ataccgctgt cgcaggtgat
    29821 gttccaggca ttgataacat taaatatccg ctgaaactgg gcaacgggcc ggggctgatg
    29881 ctgtttgaca agcgctactt ccccaaccag aaactggtag cagcgttaaa aagctgtgcc
    29941 acgcagaatg atttaccgct gcaattttcc actatgaaaa ccggtgcgac ggatggcggg
    30001 cgctacaacg tgatgggtgg cggtcgcccg gttgttgcgc tgtgcctgcc aacgcgttat
    30061 ctgcacgcca acagcggcat gatttcaaaa gccgattacg atgctctgct cacgttgata
    30121 cgggattttc tgaccacctt aaccgcggag aaagtcaacg cgtttagcca gttccgtcag
    30181 gtggattaat gctaaacgaa cgccagttaa agattgtcga tctgctggag caacagccac
    30241 gcacgcctgg cgagctggcg caacagactg gcgtttctgg taggaccatt ctgcgtgata
    30301 ttgactatct caactttacc cttaacggca aagcccgcat ttccgccagt ggcaacgcgg
    30361 gctatcagct ggaaatcttc gagcgccgca gcttttttca gttgctgcaa aagcacgata
    30421 acgacgatcg gctgctggcg ctgctattac tgaatacctt cactccccgt gcgcaactcg
    30481 cctcggcgct taatttgcca gaaacgtggg tagcagagcg cctgccccgc ctgaaacagc
    30541 gctatgaacg cggtttttgt ctcgctagtc gccctggttt gggccatttc attgatgaga
    30601 cagaagagaa acgcattatt ttgctggcga atcttttacg caaagatccg tttttaattc
    30661 ctctggcggg cgtaacacga gacaaccttc agcaattatc cacggtctgc gacaaccaac
    30721 accgctggcc gctgatgcag ggtgattatc tctccagcct gattctggcg atttacgccc
    30781 tgcgtaatca actgaccgat gagtggccgc aatatcccgg tgacgagata aaacaaatcg
    30841 ttgaacaaag cggcctgttt cttggtgata acgctgtaag aaccctgacg gggttgatag
    30901 agaaacagca tcaacaggcg caggttattt gtgtcgataa tgtgctggca ttgctgcaaa
    30961 gggtgccggg cattgcgtca ttgaatatta ttgatacgca actggttgag aatattaccg
    31021 ggcatttatt acgttgcctt gccgcgccgg tgtggatagg tgagcaccgc caaagcagca
    31081 tgaataacct gaaagccgcc tggcctgcgg cctttgatat gagtctgcac tttattacgc
    31141 tactgcgtga acaactcgat attccccttt tcgacagcga tctgatcggt ttgtattttg
    31201 cctgtgcgct ggaacggcat caaaacgaac gccagccgat cattttgctc tcggaccaga
    31261 acgcgattgc caccattaat cagctcgcca ttgagcgcga tgttttacat tgtcgggtga
    31321 ttattgcccg tagcttaagc gaacttgttg ccattcgcga agagattgag ccgttattga
    31381 tcattaataa cagccattat ttactggatg atgcggtcaa taattgcatc atcgtaaaaa
    31441 atatcattac ggctgccggt atcgaacaaa taaaacattt tctggcgacg gcatttattc
    31501 gccagcaacc ggagcgtttt ttttctgccc ccggaagttt tcattattcg aatgtacgtg
    31561 gtgaaagctg gcaacatatt acccggcaaa tttgtgcgca attagtgacg caacaccaca
    31621 ttaccgccga tgaagtacaa cggatcatcg cccgcgaaag cgaaggcgaa aacctgattg
    31681 ttaatcgtct cgccatccca cattgctgga gcgaacagga acgccgtttt cgtggatttt
    31741 ttattaccct cgcccaaccg gttgaggtga ataacgaagt agttaaccat gtgctgatcg
    31801 cctgtgccgc cgccgatgcg cgtcatgagc taaaaatatt cagctatctg gcaagcgtat
    31861 tgtgtcagca tccggcagag gttattgccg ggttaacagg atatgaggca tttatggagt
    31921 tacttcacaa ggggtgaact gtgtcggatg cgccgcatcc gacactgcat ttatcaccaa
    31981 aatcaaaaac gtttaacgca ggctattata gtcatcgacc atttcgttgt actcgcgtaa
    32041 cgctcgcgga aatgagtcgt cgaccatcca gcttgtgttt gcatcctgaa gttgctcttt
    32101 atcccagtca gagtacggaa ctttatctct gacgcggcga acatattttt tagcctcgag
    32161 ttgatactgg tctgccgaat taatgaacga gaaattcata ccgcctttat ccgcttcttt
    32221 ggcctgcgcc accagcgttt ccagttcaga gactttcttc attgccgcat caacatcaaa
    32281 cttctcttgc gaaatcaggt tattaatttg tttcgctgaa atcatgactg ccagagagta
    32341 gtagtggaat gttttccctt ctctttgttc aatatttttc agttccgcaa gctggcgctt
    32401 atcgttaatt tcctgaatcg ccgcatgata agattctgct acaggttcaa aggcttcaag
    32461 attttttaag aatgtctggt gcagcgtttt ccctttcgca aacgcatcgt ctttataatt
    32521 ttcctgggtg taatatttat ccatttcgtt aatggtatta cccaacgcca cggcagtatc
    32581 aatataactt accgccacgc catcaatagg ttgcagcgca ggcgttaacg ccactacacg
    32641 cgctttgttt ataaataaaa taataaaatt cacgaaatga ataagtcctg caatttaata
    32701 tatatcgcag gacttatttt aattaattgc tcaaacgctg cggatgggta taaaccgttg
    32761 cccgacccgg tttacaaaaa cctaccagtg tcagattaca gcgctcagcc acttctacag
    32821 caagcgtggt cgcggcagac accgcaaaca aaatctctac gccgcacatc gccgactttt
    32881 gcaccatttc ataactggca cggctggaaa ccagtaccgc accttgctgc cagctttccc
    32941 cttcttgtga ccggcggcct aacagtttgt ccagcgccac atggcgaccc acgtcttcat
    33001 gcccgccgac cagttcgcca gatggcaaca tccatgcagc ggcgtgagta caaccagtca
    33061 gttgccccac tggctggaaa tcattgagat gacgtaatgc atcatccagt ttgttgagat
    33121 caaacgtctg ggtgaacggc agcggctgca ccggtttacc gatatcgtta agctgttcta
    33181 caccgcatac gccacagccc gtacgtcccg ccagcgcccg gcggcgctcc ttcaacccca
    33241 taaagcggcg gctggaaagc tcaatttgca cttccagacc attacaggaa ggaacgacat
    33301 ccataccgaa gatatcgcgc ggactttcga taatcccctc ggaaagcgaa aaaccgagcg
    33361 caaagtactc aaggtctttg ggcgacgcca tcatcaccac atgcgaaatg ccgttgtaga
    33421 ccagcgcaac gggaacttct tccgcgacct catctaaccg tgggtgttgt aaatcatcac
    33481 gccgccataa ctcaatttga cgaacacctg tgacatttgt cacattttcg atttcttttt
    33541 gctgtgtttt tttcactttc tcttaaccag ataagaacac acacaccaac attatggtat
    33601 tctgttacaa acccttcctg gatggaggga aattgagcca attctggacc tttgcggccc
    33661 cttccgcaaa gaaaaataac tcccactccc tgcacacgca gcaagcgaat gtaaatggga
    33721 cgtgacaatg tcgaaacaag gagcaatcca tgcaggtcag cagaaggcag ttctttaaga
    33781 tctgcgctgg cggtatggca ggcaccacgg cagcggcact gggttttgca cccagcgtag
    33841 cactcgcgga aacccggcag tataaactgc tgcgcacccg cgaaacccgt aatacctgca
    33901 cctattgttc cgtaggctgt gggctgttga tgtacagcct cggtgacgga gcaaaaaacg
    33961 ccaaagcatc tatcttccat atcgaaggcg atccggatca cccggtcaac cgcggtgcac
    34021 tttgtccgaa aggcgctggc ctggtggatt tcatccactc cgaaagccgt ctgaagtttc
    34081 cggaataccg tgcgccaggt tctgataaat ggcagcaaat cagttgggaa gaggcgtttg
    34141 atcgcatcgc caaactgatg aaagaagacc gcgatgctaa ctacattgcg caaaacgccg
    34201 aaggcgtgac tgttaaccgc tggctctcca ccgggatgct ttgtgcttcc gcgtcgagta
    34261 acgaaaccgg ctatttaacg caaaaattct cccgcgcgct ggggatgctc gcggtcgaca
    34321 accaggcgcg tgtctgacac ggaccaacgg tagcaagtct tgctccaaca tttggtcgcg
    34381 gtgcgatgac caaccactgg gtcgacatca agaacgccaa cctcgtcgtg gtgatgggcg
    34441 gtaatgccgc tgaagctcac ccggtcgggt tccgctgggc gatggaagcc aaaattcaca
    34501 acggcgcgaa gctgattgtg atcgatcctc gctttacgcg tacggcggcg gtggctgact
    34561 actatgcccc tattcgttcc ggtactgaca ttgctttcct gtcaggcgta ttgctgtacc
    34621 tgctgaacaa tgaaaaattc aaccgcgaat ataccgaagc ctataccaac gctagcctta
    34681 tcgtgcgtga ggattacggc tttgaagatg gcctgttcac cggctacgac gcggaaaaac
    34741 gcaagtacga taaatcctcc tggacttacg aactggacga aaacggcttc gccaaacgcg
    34801 ataccacgct gcaacatccg cgctgcgtgt ggaacttgct gaaacagcac gtttcccgct
    34861 acacgccaga tgtggttgaa aacatctgtg gtacgccaaa agacgcgttc ctgaaagtct
    34921 gcgaatacat cgccgaaacc agtgcacacg ataaaactgc ctcgttcctg tatgccctcg
    34981 gctggacgca acactccgtt ggtgcgcaaa acattcgtac gatggcgatg atccagctgc
    35041 tgctcggcaa tatggggatg gccggcggcg gcgttaacgc cctgcgcggt cactccaata
    35101 ttcaggggct gacggacctg gggctgctgt cgcagagcct gccgggttac atgacgctgc
    35161 caagcgagaa gcagaccgat ctgcaaacct accttaccgc caacacgcca aaaccgctgc
    35221 tggaaggcca ggtaaactac tggggcaact acccgaaatt cttcgtctct atgatgaagg
    35281 ccttctttgg tgataaagcg acggcggaaa atagctgggg ctttgactgg ttgccgaagt
    35341 gggataaagg ctacgacgtc ctgcaatact tcgagatgat gaaagaaggc aaggtcaatg
    35401 gctatatctg ccagggtttt aaccctgttg cctcattccc gaacaaaaac aaagtgatcg
    35461 gttgtctgtc gaaactgaag ttcctcgtca ccatcgaccc gctgaacact gaaacctcta
    35521 acttctggca gaaccacggt gagctgaacg aagttgactc gtcgaagatc cagaccgaag
    35581 tgttccgtct gccatcgacc tgcttcgcgg aagagaacgg ttcaatcgtt aactcaggtc
    35641 gctggttgca gtggcactgg aaaggtgcgg acgcccctgg gattgcgctg actgacggcg
    35701 agatcctctc cggtatcttc ctgcgcttgc gcaagatgta tgccgaacag ggtggcgcga
    35761 acccggacca ggtgctgaac atgacctgga actacgccat cccgcatgag ccgaagtcgg
    35821 aagaagtggc gatggagagc aacggtaagg cgctggccga tattaccgat ccggcaaccg
    35881 gtgcggttat cgtcaagaaa ggccaacaac ttagctcgtt cgcccaactg cgcgatgacg
    35941 gtaccacctc ctgtggctgc tggattttcg ccggtagctg gacgccagag ggcaaccaga
    36001 tggcgcgtcg tgataacgcc gatccgtctg gcctcggtaa cacgctgggc tgggcatggg
    36061 catggccgct taaccgccgc attctgtata accgcgcctc cgcagatccg cagggtaacc
    36121 cgtgggatcc gaagcgtcag ttgctgaaat gggacggcac taagtggacc ggctgggata
    36181 ttccggacta cagcgcagcg cctccgggca gtggcgttgg gccgtttatc atgcagcagg
    36241 aaggcatggg gcgtctgttt gccctcgata agatggcgga aggtccgttc ccggaacact
    36301 acgagccgtt tgaaacgccg ctgggaacta acccgctgca tccaaacgtt atctcgaacc
    36361 cggctgcacg tatctttaaa gacgacgccg aagcattggg taaagccgat aagttcccgt
    36421 atgtcggaac cacctatcgt ctgaccgagc acttccacta ctggaccaaa cacgcgctgt
    36481 tgaacgcgat tttgcaacca gagcagtttg tggaaatcgg tgagtcgctg gcgaataaac
    36541 ttggcattgc ccagggcgat acagtgaaag tctcctccaa ccgtggctat ataaaagcca
    36601 aagcggtggt gaccaaacgt attcgcacgc tgaaagcgaa cggcaaagat atcgatacca
    36661 tcggtattcc gattcactgg ggctatgaag gtgttgcgaa aaaaggcttt attgccaata
    36721 cgttaacgcc attcgttggt gatgcgaaca cgcagacgcc agagtttaag tccttccttg
    36781 tgaatgtgga aaaggtgtaa cggagacgac atatggctta tcaatctcaa gatatcattc
    36841 gtcgttccgc gactaacggt ctgacccccg cgcctcaggc gcgggacttc caggaagaag
    36901 tggcgaaact catcgacgtc accacctgta tcggctgtaa agcctgtcag gtggcgtgtt
    36961 cagagtggaa cgacattcgc gataccgtcg gcaataacat tggggtgtac gacaacccca
    37021 atgatttaag tgccaaatcg tggacggtga tgcgcttctc ggaagtggaa cagaacgaca
    37081 aactggaatg gctgatccgc aaagacggct gtatgcactg ttccgatcca ggctgcctga
    37141 aagcgtgccc ggcggaagga gcaattattc agtatgccaa cggtatcgtc gacttccagt
    37201 ccgagcagtg catcggctgc ggttattgca ttgcgggctg tccgttcgac attccgcgcc
    37261 tcaacccgga agacaaccgc gtctacaaat gtacgctgtg cgttgaccgc gtggtggttg
    37321 ggcaagaacc ggcctgcgtg aagacctgcc caacgggcgc gattcacttc ggtacgaaag
    37381 agtcgatgaa aacgctggcg agcgagcgcg tggcggagct gaaaactcgc ggttacgaca
    37441 atgcgggtct gtacgatccg gcaggcgttg gtggtacaca cgttatgtac gtgctgcacc
    37501 atgctgacaa gccaaatctg tatcatggct tgccggagaa cccggaaatc agcgaaaccg
    37561 tgaaattctg gaaaggcatc tggaaaccgc tcgcagctgt tggctttgcg gctaccttcg
    37621 cagccagtat cttccactac gtgggtgtcg gtccgaaccg tgcggatgag gaagagaata
    37681 atctgcacga agagaaagac gaggagcgca aatgaaacga cgtgacacca tcgtgcgcta
    37741 caccgcgccg gaacgtatca accactggat caccgccttc tgcttcatcc tggcggcggt
    37801 gagcgggctg ggctttttgt tcccgtcctt caactggttg atgcaaatca tgggcacacc
    37861 gcagctggcg cgaattctgc acccgtttgt cggcgtggtt atgtttgcct cgttcatcat
    37921 catgtttttc cgttactggc atcacaacct aatcaatcgg gatgatatct tttgggcgaa
    37981 gaatattcgt aagatcgtcg tcaacgagga agtaggtgac accgggcgtt ataacttcgg
    38041 tcagaaatgc gttttctggg cggcgattat tttcctggtt ctgctgctgg tgagcggcgt
    38101 gattatctgg cgtccttatt ttgcgcctgc tttctcaatc ccggtgatcc gattcgcgtt
    38161 aatgctgcat tcatttgccg cagtggcgtt aattgtggtt atcatggtgc atatctacgc
    38221 cgccctttgg gtgaaaggca cgattaccgc catggtggaa ggatgggtta ccagcgcctg
    38281 ggcgaagaaa catcacccgc gctggtaccg tgaggtccgc aagacaacgg aaaagaaagc
    38341 tgaatgagta ttcgcataat cccgcaagat gagctgggtt cgagcgagaa acgtacggcg
    38401 gatatgattc cgccgttatt gttccctcgg ctcaagaatt tatacaaccg ccgcgccgag
    38461 cgtctgcgcg agctggcaga aaataatccg ctgggtgatt acctgcgctt tgctgcgctt
    38521 atcgcccacg cccaggaagt ggtgctgtac gaccatccgc tggagatgga tctgaccgcg
    38581 cgcattaaag aagccagcgc acagggcaag cccccgctgg atattcacgt tctgccgcgt
    38641 gataagcact ggcaaaagct gctgatggcg ctgattgctg agctgaaacc tgaaatgagc
    38701 ggcccggcgc tggcagtgat tgagaatctg gagaaggcat cgactcagga gctggaagat
    38761 atggccagcg cactgtttgc ctctgatttc tcgtccgtca gcagcgataa agcgccgttt
    38821 atctgggctg cgctgtcgct ctactgggcg cagatggcta atctgatccc cggcaaagcc
    38881 cgcgctgaat acggcgaaca acgtcaatat tgcccggtat gtggctctat gccggtgtcc
    38941 agcatggtgc aaattggcac cactcagggt ctgcgttacc tgcactgcaa cctgtgtgaa
    39001 accgaatggc acgtagtgcg cgtaaaatgc agcaactgcg agcagagcgg caaactgcat
    39061 tactggtcgc tggatgacga acaggccgcg attaaagccg aaagctgcga tgactgcggc
    39121 acttacctga agattcttta tcaggaaaaa gaaccgaaag ttgaagccgt ggcagatgac
    39181 ctcgcctctc tggtactgga cgcgcgaatg gagcaagaag gctatgctcg tagttccatc
    39241 aacccgttcc tgtttccggg tgaaggggag taatctctga cgttttccgg gtggcgcagg
    39301 tcgcccggat tacagttcat agggtgtgac gacaccatct ctcttattcc gcgttaccac
    39361 ctcaagctat gctgccagtg cctgttttgc ctcaattttc acactgaaca aatcgataac
    39421 aatatcgata tcaaatagcc aaatcttttt gccattacca ttcctcgcgc agcttacgct
    39481 ggtattggac accatcttcc tcactccctt gccagatgcc aaaagcactg gctcttgctg
    39541 tttgcgattg atttaccagg tattgatcta ccgcttcccg taatagctcc gcacgaggaa
    39601 gattatgctg cacctcgaga tcatcaagtt gcttaaacaa ctcattcgat aaattgagta
    39661 aaattctgcc catatctata cctgccagag agttcataaa tatctcgccc atatcatttt
    39721 tgaatctatg gagagaaaag tacccttgtc gaatctttaa agaaagcgta tttacgcatc
    39781 actttttatt tttgcgaggc gttttccaga tatgatgtaa cagttgcagt tattataagt
    39841 tcaagtaaaa cagccttgta ttaataccag aataagggca tctgtttttt tatattcaag
    39901 aatgaaaaat ttttgtcatt ccttatgctc cttacactga tcatcaacac caactacctc
    39961 ccccttttgc ctgcctccct ttgctttacg caccgggaat tgctgacgtt ccagatattg
    40021 ctctaccgct tcgcgtaaaa ttgcagatcg tgaaacctta cgccagcccc taagcttatt
    40081 cagccgttta attgcctctt ctgataaatg aagaaaaacc gtattcatcg ccattaccac
    40141 caccttacat atattactca ttaatgtatg tgcgaaaatt acgccgaaaa atcatttaaa
    40201 aaaagcggat ttttgcatgt cgttttgttt ttgcgagacg gcatccaaag ataatgtaac
    40261 agttgcagtt atttcaattg ttactcaaaa atggtttagt aataacccag gatggtttat
    40321 tacttttaat taagcgggga cacatctaca gaaaagaaac aacaaataat aaggatgaat
    40381 tatgggtgct ataaatattt tatattcaag aatatttttt tgattgggtg ttcatttgct
    40441 tcatcaggat ggtgtacccg aaaaagtcag cagctttacc gcaccagggt ttaaggtgaa
    40501 atctaccatt cataactgac cggtcagtgc cgcaccaacc ggtctttaat tgttagtagt
    40561 agtaaatttg tggataacag tccgggaacc agacgattcc ttctggatca tccccctgca
    40621 ttacccgatg acattggcgt cgatcatctg cgcgacggcg aagcgtagta tagagccgct
    40681 tccattcatg atcgcccggt gtactgtagt ttacccaaca atccattgaa gggtgacgcc
    40741 atttacctgt aagtgttggg atccctgaat gattatgtgg cttcgcttta ccattacgat
    40801 ccctatcttt acgcgtatta agcgtggaca tttttagtct cctgttaaga gttctaaaaa
    40861 tgtgcttcct taaaatagtt catcattttc ccttaattcg catagcgtgc ttttgcacga
    40921 atttccgata cgcggggacc attatgatag cgtcgatgcc cacacatcca acaagaacaa
    40981 atacagggag tacgaaagca aatacctgat gcttttttgc ttccatcgcc tgcagtgcga
    41041 taacggcagc ggattttttt taatcgcgcc atatgatggc gacgttcagc acgattacgg
    41101 gtcatagtaa aactcctgag caggcggcac tatgccgctt tcataggagt tctgaatgct
    41161 ggtgctttct gtatggcata accatgcctc taacggttaa gtgcctgcag cacatgttgc
    41221 ggatcattgg caatcgctcg cagcaaagcc tttgcaggcc cagtcggtga acgtcgcccc
    41281 tgctcccagt tacgcaacgt gtctacgctt acggaaataa gttcggcaaa tttagattgt
    41341 gacaaccctg aagcgcggcg tatttctttg attttcatag catcaacttc aaaagtccgt
    41401 gaagggtgtc gcgttccttc aataatttca ttcatctggg ccatgctgtg agtcaaccgc
    41461 tcaaaaagtg ccttatccat ttaccacctc gtatttaaca accggagcat tgctttttcc
    41521 tggggagaca gatcatcttt aataccctta cgataaataa gcagtaatct gatctcataa
    41581 tgtttaacct gatgaaagta aataaccctg acaccagcac gtttcccctt tcctcctgac
    41641 acccatcgaa cctttcttaa cccaccagtt tcaggtatta cctcaccata gtcaggattc
    41701 aaggccagga aaaactgaaa acgactgaat tcatcgtcgg tcagcagttt ttgaacatct
    41761 tcagtaaaaa tttccgtttc gatgaagagc ataattatac gccattggct tagagtaaca
    41821 atcctttttg tacgtcattg acgtaccctt gaaaccacta gcttactgat agtgctccag
    41881 caaaaccacg tctctgccgg gtttgtcttt acatttgcga ggcgctttcc agatttttca
    41941 cgcccgtgaa tttccctgta tccatccagc aaaaacgggt gtataaaact gtacattcat
    42001 acagcaaaat gagttctact atggcactgg aaaaaggaat tgcagagtta gtcgaagagt
    42061 ttatcgctgc gggtcgcccc tcatcgcgtc aacaaagtat tgcgcagcgc agggagggct
    42121 atatcgccag cgctgtcctt gcgggtgaga cagaaacgcg ggtagatatt cagactatcg
    42181 aacttgaagg gatgatctta cgaatagtgt caccgctgaa tgccccgaca ttactcccga
    42241 cgataattta ttactacggt ggctgttttg ttagcggcgg gttcgcaact cacgacaacc
    42301 agctgcgcca gttagcctat tacgggcaat gtcgggttat tgccgtccag tatcggctgg
    42361 caccggaaca gactttcccc gccgcccatg atgatgcgca acgaggtgca gaattagtct
    42421 ggcaacacgc cgaacgactc ggcgttgata aacaacggat aactcttgcc ggtgacagtg
    42481 cgggcggaca tctggcgctg gtaactgctt tgcgattaaa gcgtgctggc gagtggcaac
    42541 cggcacaact catcttaatt tatcccatgc ttgatgcgac tgcgcatttc gaaagctaca
    42601 tacgcaatgg tcacgattac atcatcacac gcgacacttt actgagcggc tttgagatgt
    42661 atctgccagg cattgaacgc aggcatcctg aagccagccc tctttggcgc gatgatttca
    42721 atggcttgcc acctgtacat atcattaccg ctgaatacga cccactttgc gatgaaggtg
    42781 aagcgttgta tcaccgcatg acagggcaag gagtgcaatg cacctgccaa cgctaccttg
    42841 gcgttatcca cggatttttt cagttaggtg gtatcagtga ggctgcgcga tcggcgctgc
    42901 gggatgtggc ctggagagcg ggaaggtaac tcctgt
//
LOCUS       DAPOZO010000037        42861 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPOZO010000037 DAPOZO010000000
VERSION     DAPOZO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.037"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(81..2585)
                     /gene="copA"
                     /locus_tag="ROI21_002131"
     CDS             complement(81..2585)
                     /gene="copA"
                     /locus_tag="ROI21_002131"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDX5111050.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHAVLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            2848..3780
                     /gene="glsA"
                     /locus_tag="ROI21_002132"
     CDS             2848..3780
                     /gene="glsA"
                     /locus_tag="ROI21_002132"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDX5111051.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            3783..5075
                     /locus_tag="ROI21_002133"
     CDS             3783..5075
                     /locus_tag="ROI21_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDX5111052.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            5472..6767
                     /locus_tag="ROI21_002134"
     CDS             5472..6767
                     /locus_tag="ROI21_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HDX5111053.1"
                     /translation="MVMGEAAIAAPLPQWANAPAVTPVAQLSLQESILRAFARNPDVT
                     QQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDSSGRLDNNVSYGITLTQLVYD
                     FGKTNNDIKLQTAARDSYRFKLMATLTDVAEKTATAYMEVSRYQALCDAAQRNIHSLE
                     NVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQAQMASAKAQLAVLTGVQPEA
                     IAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQYGVEKTKAQYWPTLSIQGGKT
                     RYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQQKISASQVEQAKLDVLQRAS
                     VAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGKRSLNDLLTVEQDVFQAQSAE
                     INANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDLK"
     gene            6876..27653
                     /locus_tag="ROI21_002135"
     CDS             6876..27653
                     /locus_tag="ROI21_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDX5111054.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVS
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHVTFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDATGNTVTGAQLLTVDTQPPTLAINTIAQDNIVSAAEH
                     NAALVLSGTSNAEAGQTVTLTVNGKSHSVTVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSNSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDTAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPTLSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQSDGTWSVTVPATNVDAL
                     ADGNATVTASVNDVAGNPTSVSRVALVDATPPVVTINPVATDNVINTPEHTQAQIISG
                     TVTGAQAGDIVTVTLNNSDYTTVVDGSGNWSLGVPASVVSGLVDGSYPVSVSVTDRAG
                     NSGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVNTAITVTLNG
                     QNYTTTTDASGNWSVTVPASAVTALGQANYTVTAAVTSSIGNSNTASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAEDGDTVTITLGGNTYTATVGSNLTWSVS
                     VPAADIQALGNGDLTVSASVTNQNGNTGSGSRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVITGSSSGLAEGTLLTVTINNVEYITAVQADGSWSVGVTAAQVSAWPAGTVSI
                     AVSGESSAGNPISITHPVTVDLTPAAITINTIATDDVINGAEKGADLTLSGTTTNVEA
                     GQTVTVTFGGKNYTASVASDGSWSATVSAADLALLTDGSASAQASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTVTLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINAAEHGQALVISGSSTGGEAGDVVSVTLNNKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDSETHTVTVNLTAPTIGINTIATDDVINATEKGAD
                     LQISGTSNQPSGTTITVTLNGQNYTATTDASGNWSTTVPASAVGALGEASYTVTANVT
                     DSAGNSNSASHNVQVNTALPGVTLNPVASDDIINAAESGVAQTISGQVTGAAAGDTVT
                     VTLGGKTYTATVQGNLSWSVDVPAADIQAIGNGDLTVNASVTNGVGNTGSGSRDIVID
                     ANLPGLRVDTVAGDDVVNSIEHGQALVITGSSSGLAAGAALTVVINNVTYGATVLADG
                     TWSLGVPAADVGNWPAGTVNIIVSGTNTAETTTTITHPVTVDLAAVAISINTVSGDDV
                     INAAEKGADLTLSGSTSGVELGQTVTVTFGGKTYTATVAGDGSWTTIVPAADLSALRD
                     GDASVQASVSNVNGNTASATHAYSVDATAPTLAINTIATDDILNAAEAGNPLTISGSS
                     TAEAGQTVTVTLNGVTYTGTVLADGSWSVSVPTADLSNLTASQYTVSASVSDKAGNPA
                     TATHGLAVDLTVPVLTINTVSGDDIINAAEHGQALVISGSSAGGEAGDVITVTLNSKT
                     YTTTLDASGNWSVGVPLSDVTALGSGPQTITATITDAAGNSDDASRTVTVNLTAPTIG
                     INTIASDDVINATEKGADLQITGTSNQPAGTTITVTLNGQNYTATTDASGNWSATVPA
                     SAVSALGEANYTVTANVTDTAGNSNSASHNVLVNSALPAVTINAVATDDIINAAESGN
                     AQTISGQVTGAAAGDTVTVTLGGNTYTATVQANLSWSVSVPAADIQAIGNGSLTVNAS
                     VTNVVGNTGNGSRDITIDANLPGLRVDTVAGDDVINSIEHNQALVITGSSSGLTAGTA
                     LTVEINNVTYGATVLADGTWSLGIPAADVSNWPAGTVDITVSGTNSAGTTSTITHPVT
                     VDLAAVAITINTLSGDDVINAVEKGETLVVSGSTSGVEAGQTVTVTFGGKSYTTTVEA
                     NGSWTVNVPPADLASLPDGAGNVQASVSNINGNSAQADRAYSVDATAPLVTINTIASD
                     DILNVSEAGAGITISGTTTAQVGQTLTVTLNNNTYQTTVQADGTWSVNVPATDLSGLT
                     ASSYTVTATVSDKAGNPASADHALAVDVTAPDLTINTVAGDDIINAIEHGQALVVSGT
                     STGAAAGDVVTVNLNGKNYTTTLDASGNWSVGIPAADVTALSTGSQTITASLSDRAGN
                     SDSTTHDVTVDLSGPTLSINTVSGDDIINNAEKTQDLTISGGSSGLATGTTVTVMLNG
                     LAYSATTDGTGNWSVTVPASAVGALGEAVYQISASATDSAGNSGSTTHTVKVESLLPG
                     VIINTVAGDDIINAAEIAVNQTISGQVSGTAVAGNTVIVTIGGNQYTAIVQSDLSWSV
                     SVPANVLQALGNGELTISASVTNGVGNTGTATHDIVIDANLPGLRVDTVAGDDVINSI
                     EHTQALVVTGSSSGLAAGAALTVVINGVTYGATVLVDGTWSVGVPAVDVTNWPAGTVN
                     IAVSGTNTAGTTTSITHPVTVDLAAVAITINPLSTDDVINAAEKGSDLQLSGTTSGVE
                     AGQTITVIFGGKSYTTTVAADNSWGLTIPAADLATLPDGAANVQASVSNVAGNSAQAT
                     HAYSVDATAPSVTINTIATDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGN
                     VQADGSWSVSVPTGDLANLTASPYTVSAAVSDKAGNPASATHNLTVDLAAPVVTINTV
                     AGDDIINATEHSQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVI
                     SALAQGDVTITATVTDSAGNSGTASHSVSVALGAPVLSINTIAVDDIINATEKSADLA
                     ISGTSDQPAGTQITVTLNGQNYTTTADASGNWSVTVPASRVSALGEATYTVTAAATDS
                     DGNSGSANHNVQVNTALPGVTINVVASDDIINAAEAGAGQSISGQVTGAAAGDTVTVT
                     LGGATYTATVQANLSWSINVPASALQALGNGELTISASVTNSVGNTGNGTREITIDAN
                     LPGLRVDTVAGDDVVNIIEHGQALVITGSSSGLATGSNVTLTINGQTYVAAVLADGSW
                     SVGVPAVDVSAWPAGTVTITASGSTTAGNSVSVTHPVTVDLSAIAVSINAITADDVIN
                     AAEKGAALTLTGSTSGVEAGQTVTVTFGGKTYTASVAANGSWSTTVPAADMAALRDGD
                     ASAQASVSNVNGNNATTTHAYSVDASAPTVAINTIAGDDILNAAEAGAALTITGSSTA
                     EAGQTVTVTLNGENYTGTVQTDGSWSVSVPPADVSALTASNYTVSAAVSDKAGNPASV
                     NHNLTVDTSVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGSSTFT
                     TVLDASGNWSVGVPASVVSALANGTVTINASVTDAAGNSGSATHLVTVNTGLPSITFN
                     AISGDNVLNADEKGQPLTISGSSTGLATGAQVTVTLNGHNYSATTDAAGNWTLTVPVS
                     DLAALGQANYIVSASATSVAGNTASSQANLLVDSGLPGVTIDTVAGDDIINAAEAGDD
                     QTISGVVTRAAAGDTVTVTLGGNTYTTTVQSNLSWSVTVPAADLQALGNGDLTITASV
                     TNANGNTGSGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAAL
                     TVAINGVAYAATVQADGSWSVGIPAASVSAWPAGPLTVEVTGQSSAGNPVSASHPFTV
                     DLTAVAISINTVASDDVINAAEKGTGLTLSGSTSGIESGQTVTVIFGGKTYTASVAAN
                     GSWSVNVPAADLATLPDGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDD
                     ILNATEAGSPLTISGTSTAETGQTVTVTLNGATYSGNVQADGSWSVSVPPSALGALSA
                     SNYTVSATVNDKAGNPGSASHNLAVDTTASVLTINTVAGDDIINDAEHAQALVISGTS
                     TGGEAGDVVSVVLNGKTYTTTLDASGNWSVGVPAADVAALGSGAQTITASVSDRAGNS
                     DDASRTVTVSLSAPVISINTIAGDDVINAMEKGSDLALSGTSDQPAGTAITVTLNGQN
                     YSATTDASGNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGVT
                     INPVTTDDIINAAEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVP
                     AADWQALGNGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHA
                     QAQVITGSSSGFAAGTALTVVINNQTYAATVLANGSWSVGVPATDVSNWPAGTLNITV
                     SGANSAGTQTSITHPLTVDLTTVAVSINSITSDDVINAAEKGAALTLSGSTSGVEAGQ
                     TVTITFGGKTYTTTVAANGSWSTTVPAADMAALRDGDASAQVRVTNVNGNSATATHEY
                     SVDSAAPTVTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVKA
                     DGSWSLTLPASDLTALANNGYTLTATVSDQAGNPGSASKGVTVDTTAPVISFNTVAGD
                     DVINNVEHTQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGL
                     ADGTVTISATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNIINAAEAGSALTLIG
                     TGTNFAAGTVVTVLLNGKGYSATIQNNGSWSVNVPAADVAALADGTSYTVSASAQDSA
                     GNSATASRSVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFG
                     DKTYTATVAANGTWALNVPAADLAALGQGAQTITASVNDRAGNPGQTTHALTVDTVAP
                     TVTIATVAGDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQSWTATVGSGGSWSVF
                     IPAQQFAGLSDGSYTISATVSDQAGNPGSASRGVTLNGDVPSVTINTFAGDDVVNAAE
                     HGSSLVISGTTTAPIGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVI
                     NASVSNAIGNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSL
                     TAALASNETAQISIDGGVTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGA
                     TDSQNVVIDTTAPDPAVKTIAISAITTDTGLITNDFVTSDTTLAVSGTLGAALSAGEF
                     AQISIDGGTTWQNLSVSGLTWTWLDGRTLTDGNYNYQVRVIDTAGNIGATASQIVTVD
                     TTAPLASKTIAIAGISDDTGLSSSDFVTRDTTLTVRGTLGAALAADERAQISLDGGVT
                     WTTLTVIGTSWSYADGRTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAKSI
                     TITGISDDTGASSSDFITSDTSLTVRGVLGAALGANEFAQISTDNGATWVNVTVAADG
                     LNWSYVDGRTLTNGTTTWQVRVVDLAGNVGATGSQSAQIDTVNPAQVLTIASISTDTG
                     SSATDFITSDTSLTLTGSLGAGLASGEVAQISLDGGATWITLTTNGTQWTYTDGRTLT
                     DGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQAT
                     DDTTPLLNGVLSGPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYSAR
                     VVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVEVV
                     INGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNANI
                     SNGTVTVNAAIDYTPTWTTTSKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWSKT
                     ALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQVTV
                     GTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSATV
                     GGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNTTN
                     TFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPVAV
                     GATATTYASQFSVAVDWNHDGLMDIARIAQTGQSYLYTNVSNASNWTQSALGSSQSGT
                     TSGVAAMDYDWDGAVDVLVTKQSGSVYLIRNTNTVSYGTSLHLRITDPNGINVYYGNT
                     VKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASNID
                     QTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSGGW
                     VYSSGTGTWLANGGMDVVDFRLSTVGVTANLSVTTAQATGFNTSTFTNIEGISGSNFN
                     DTLTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVGTW
                     EGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTIVQ
                     IDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMLV"
     gene            27657..29819
                     /locus_tag="ROI21_002136"
     CDS             27657..29819
                     /locus_tag="ROI21_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDX5111055.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADIEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWNLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAIFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLVIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQSEPTTRRVVLQEKNKGSAA"
     gene            29816..30991
                     /locus_tag="ROI21_002137"
     CDS             29816..30991
                     /locus_tag="ROI21_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDX5111056.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYAGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKHKQQFPIFPGMVATVDIRTGKKSVLDYLLRPFNKAQEALRER"
     gene            30988..31395
                     /gene="cueR"
                     /locus_tag="ROI21_002138"
     CDS             30988..31395
                     /gene="cueR"
                     /locus_tag="ROI21_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDX5111057.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(31599..32021)
                     /locus_tag="ROI21_002139"
     CDS             complement(31599..32021)
                     /locus_tag="ROI21_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111058.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKSIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(32075..33091)
                     /locus_tag="ROI21_002140"
     CDS             complement(32075..33091)
                     /locus_tag="ROI21_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HDX5111059.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPPVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLTNTQKRVADNSQQINTLNNHFDSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDLMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            33458..33820
                     /locus_tag="ROI21_002141"
     CDS             33458..33820
                     /locus_tag="ROI21_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111060.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKNMNKGSDE"
     gene            complement(34005..34463)
                     /locus_tag="ROI21_002142"
     CDS             complement(34005..34463)
                     /locus_tag="ROI21_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDX5111061.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(34460..35377)
                     /locus_tag="ROI21_002143"
     CDS             complement(34460..35377)
                     /locus_tag="ROI21_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDX5111062.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            35523..36200
                     /gene="fetA"
                     /locus_tag="ROI21_002144"
     CDS             35523..36200
                     /gene="fetA"
                     /locus_tag="ROI21_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDX5111063.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALLDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            36187..36966
                     /gene="fetB"
                     /locus_tag="ROI21_002145"
     CDS             36187..36966
                     /gene="fetB"
                     /locus_tag="ROI21_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDX5111064.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(37029..37883)
                     /gene="cnoX"
                     /locus_tag="ROI21_002146"
     CDS             complement(37029..37883)
                     /gene="cnoX"
                     /locus_tag="ROI21_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDX5111065.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(37944..38753)
                     /gene="ybbO"
                     /locus_tag="ROI21_002147"
     CDS             complement(37944..38753)
                     /gene="ybbO"
                     /locus_tag="ROI21_002147"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDX5111066.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(38743..39366)
                     /gene="tesA"
                     /locus_tag="ROI21_002148"
     CDS             complement(38743..39366)
                     /gene="tesA"
                     /locus_tag="ROI21_002148"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDX5111067.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            39337..40023
                     /locus_tag="ROI21_002149"
     CDS             39337..40023
                     /locus_tag="ROI21_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5111068.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            40020..42434
                     /locus_tag="ROI21_002150"
     CDS             40020..42434
                     /locus_tag="ROI21_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5111069.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVVQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         9828 a        11987 c        12169 g         8877 t
ORIGIN      
        1 gccagtcact ttatatggca ttactcataa aaggagcgca acaagaactg gatctcacaa
       61 acgcattaag taaataccaa tcattccttc ggtttaaacc gcagcaatcg gttggcgtta
      121 ctcactacgg taatcgacga gagcgccatt gccgctccgg caaccaccgg gttaagcagt
      181 gttccagtga acggccacaa aataccggcg gcgaccggaa taccaatgct gttgtagata
      241 aacgcaccga gcaggttctg cttcatattg cgcagcgttg cgcgggaaat cgcgagcgca
      301 tctgcaacac ccatcaggct atggcgcatc agggtaatcg ccgcggtttc aatggcaaca
      361 tcactgccgc cacccatcgc aataccgaca tccgcctgag ccagcgctgg cgcgtcgtta
      421 atgccgtcgc ccaccattgc cacctgacgt ccttcacttt gcagacgttt gatcgcttcg
      481 gctttaccgt ccggtagcac cccggctatc acctcatcaa tccctgcttc tttggcgatc
      541 gcattggcgg tggttgggtt atccccggtt agcatcacca gacgatatcc cgctttatgc
      601 aggcgttgca gcgccgccac gctatcacta cgcaatggat cgcgtacagc cagcagggct
      661 accgctttcc cgtcaaccgc cagcagcaca ggcgttgccc cttgcgatgc ctgagctgaa
      721 atatctgcct caatcgcttt agtatcaact tgctggtcat ttaacagcgc ctgattgccc
      781 agcaataccg catgaccttc agcttcaccg ctcacaccca gcccgcgcaa tgtgcggaaa
      841 ccgttgacct gcggtagctg catatcacct gctttatcga ggatcgcccg tgccagcggg
      901 tggctggaac cttgctccag cgccgccgcc agacgcaatg cctgcgcttc atcaacatca
      961 gcaaacgttt ttactgcgac aacctgcggt ttaccttcgg tcagcgtccc ggttttatcg
     1021 aacactacgg tgtcgagcgt actggcgcgt tgcagagcgt cggcgtcccg caccagcaca
     1081 ccaaactcag ccgcccgccc gacgcccgaa ataatcgaca tcggcgtcgc cagtcccagc
     1141 gcacacggac aggcgataat cagcaccgtg gtggcaatca ccagggtata gacaatctgc
     1201 ggtgccggac caaagaaata ccagattgcc gcactgatca gcgcaatgac taccaccacc
     1261 ggcacaaaca cagcggagat tttatccgcc agttggccga tttcaggctt gctgctctgg
     1321 gcctggcgca ccatgcgaat gattcgtgac agcgtagtat ggctgccgac cgcactggca
     1381 cgaaacagca cactgccgtc ctgtaccact gtcccggcat ggacgctatc gccttcgcct
     1441 ttttgctgcg ggattggttc gcccgtcagc atcgcttcat ccagccacgc ttcgccctgg
     1501 gtaatctcgc catctaccgg cacgcgatcg ccggtcgtca ggcgcagcaa catacctggc
     1561 tgcacttccg ccagcggcac gcttttttca ccttcgtcag ttaccaggcg cgccgtcggt
     1621 ggggttaaat cgagtaattt ctccagcgcc tttgaagaac gctgacgtgc gcgcgcttcc
     1681 agcatatgac cgagattgat cagaccgata atcatcgcgc tggcttcgta ataaagatgt
     1741 cgcgcttcca tcgggaacca ctgcggccac aggttgacgc tcatcgaata gagccacgcc
     1801 acgccagtac ccagcgccac cagcgtatcc atcgtcgctg caccgttcag caggcttttc
     1861 catgcgctgc ggtaaaaatg cccgccggcg aaaaccatta ccgccagggt tatcaggccg
     1921 ataaccaacc acaggctgcg gttgtcggcg gtgaccatca tgttatcgcc gatcatcccc
     1981 cagaccatca ccgggatacc caccgccagt gcgacaatcg cctgccagcg gaagcgcttc
     2041 atcgtagcga cggcggtttc ttgctggcgc tcgcggcgtt tagcgtcatc ttcaatcgct
     2101 tccgcgccgt agcccgcttt ttccaccgcc tgcactaaat cttgtgggga ggcactgccc
     2161 atcaccagcg cagtacgctc cgccaggttt acccgtgcct gagtgacgcc cggtacgctt
     2221 tgcagcgcat tttgtacgcg ggtgacacag ctggcgcagc tcatgccgct cagcagcaac
     2281 tgctggctgt catcgtcatc ggcggtcgct gccggaagcg cctcagaaac cgctgtcagt
     2341 gcttccgacg ggattgatga ctccgccagc ggtttagcct ttgggtggct tacagatgcg
     2401 tcataacccg cttgtttgat ggtttcgatc agctgttctg cactggcagt cccggtaacg
     2461 tgcgcttcag tgacagacac atccgcctgc tcaacatccg ggcgctgttc gagactttct
     2521 ttcacgcgtt taacgcagtg accgcaggac aggccgtcca gggtcaggtc gatagtttgt
     2581 gacataaaac actcctttta agacagtttt gactggctgt gataaaggtt aaaccttcca
     2641 gcaaggggaa ggtcaagaaa ttaataaacc aggcgggtaa aagtccgtaa agattaaaaa
     2701 atcggctcga tttgcatcag gattagacat ttatctcttt gttttcctgt agttaagttg
     2761 cgggtgctaa gttaaatcag gatgcctgaa aatcggcacc ggggtgagga attacctccc
     2821 gcatctataa aaaggagtta acaaaagatg ttagatgcaa acaaattaca gcaggcagtg
     2881 gatcaggctt acacccaatt tcactcactt aacggcggac aaaatgccga ctacattccc
     2941 tttctggcga atgtacctgg tcaactggca gcagtggcta ttgtgacctg cgatggcaac
     3001 atctatcgcg cgggtgatag tgattaccgc tttgcgctgg aatccatctc gaaagtctgt
     3061 acgttagccc ttgcgttaga agatgtcggc ccgcaggcgg tacaggacaa agttggcgct
     3121 gacccgaccg gattgccctt taactcagtt atcgccttag agttgcatgg cggcaaaccg
     3181 ctgtcgccac tggtaaatgc tggcgctatt gccaccacca gcctgattaa cgctgaaaat
     3241 gccgaacaac gctggcagcg aattttacat atccaacaac aactggctgg tgagcaggta
     3301 gcgctctctg acgaagtaaa ccagtcggaa caaacaacca acttccataa ccgggccatt
     3361 gcctggctgc tgtactccgc cggatatctc tattgtgatg caatggaagc ctgtgacgtg
     3421 tacacccgtc agtgctccac gctcatcaat actgttgaac tggcaacgct tggcgcgacg
     3481 ctggcggcgg gtggtgtgaa tccgctgacg cataaacgcg ttcttcaggc cgacaacgtg
     3541 ccgtacattc tggctgaaat gatgatggaa gggctgtatg gtcgctccgg tgactgggcg
     3601 tatcgcgttg gtttaccggg caaaagcggt gtaggtggcg gtattctggc ggtcgtccct
     3661 ggcgtgatgg gaattgccgc gttctcacca ccgctggacg aagaaggcaa cagtgttcgc
     3721 ggtcaaaaaa tggtggcatc ggtcgctaag caactcggct ataacgtgtt taagggctga
     3781 tcatgatgaa cacggaaggt aataacggta acaaacccct cggtttatgg aacgtcgttt
     3841 ccatcggcat tggggcaatg gtgggggcgg ggatcttcgc gctgctgggg caggctgcgt
     3901 tgctaatgga agcctcgacc tgggtcgcct ttgcttttgg cggtattgtg gcgatgtttt
     3961 ccggttatgc ctatgcgcgc ctgggggcga gctatcccag caatggcggt attatcgact
     4021 tctttcgtcg cggattaggc aacggcgtct tttcgctggc actctcgtta ttgtacctgt
     4081 tgacgctggc ggtgagcatc gccatggtcg cccgtgcttt tggcgcttat gccgtgcagt
     4141 ttttgcatga aggcagtcag gaggagcacc ttatattgct ctacgcgttg gggatcattg
     4201 cggtgatgac gcttttcaac tccttaagca accatgcggt agggcggctg gaagtgatcc
     4261 tcgtcggcat taaaatgatg atcctgttat tgctgatcat tgcaggtgtc tggtcgctgc
     4321 aaccggcgca tatttctgtc tctgcgcccc ccagctccgg tgcgttcttc tcctgtatcg
     4381 ggataacctt ccttgcctat gcgggctttg gcatgatggc gaacgcggcg gataaagtga
     4441 aagatccgca ggtcattatg ccacgggcgt ttctggtggc gattggcgtt accacgttgc
     4501 tttatatctc gctggcgctg gttttgctta gcgatgtatc ggcattagag ttagaaaaat
     4561 atgccgatac cgccgtagcg caggcagctt ctccgctgct cgggcatgtg ggttatgtga
     4621 tcgtcgtcat cggcgcttta ctggcgacgg cttcagccat caacgcgaac ctgttcgccg
     4681 tgtttaacat catggacaac atgggcagcg aacgcgaact gccgaagcta atgaataaat
     4741 ccctgtggcg gcagagtacc tggggcaaca tcattgtcgt ggtgttgatt atgctgatga
     4801 cggcggcact aaatttaggc tcactcgcca gcgttgccag tgccaccttt ttgatctgct
     4861 atctggcggt gtttgtggtg gcgatccgcc tgcgtcatga tattcacgcc tcgttgccga
     4921 ttcttatcgt aggtacgttg gtgatgttgt tggtgatcgt tggctttatc tacagcctgt
     4981 ggtcccaggg cagccgtgcg ttgatatgga ttattggtgc aatcttactc agccttattg
     5041 tggcaatggt catgaagcgc aataaaaccg tataacacct ctctgcgcgc agtactgcct
     5101 gtactgcgcc tttgtcttta attcaaatta cctttcttat tcctttaata aataagtcac
     5161 aaaaaaatga tatatacgcg ataaatctgt gatgctaatc acttattacc gtggattatt
     5221 cttaatgtgt atgatggtaa tctaatagtc gtagtgtatt ctaaataaat aacgtataag
     5281 ctttttagct gcatttcttt gcccatatca atgatcaaca aaagagatgc acaagttatt
     5341 acccttctta ataaaatgag attggttata aaaaccagtt aaaataatga cctggaagga
     5401 agtgacttat ctatggcgcg attccagttt aaaaaccgta aaaataatgg acttattttt
     5461 ttataagttt tatggtgatg ggagaagctg caattgctgc accgctgcca caatgggcga
     5521 atgccccagc ggtaacgcca gttgcccaat tatctttaca ggaaagtata ttacgcgcct
     5581 ttgcgcgaaa tcccgacgtc actcaacagg cggcgcagat aggcattggc gaagcgcaaa
     5641 ttgatgaagc caaaagtgcc tggtatccgc acgttggatt aaccggcaat gcgggaccgt
     5701 cccgacaaac ggactccagt ggcaggcttg ataacaacgt ttcgtatggc ataaccctga
     5761 cacagctggt gtatgacttt ggtaaaacta acaacgatat caaactgcaa actgccgccc
     5821 gtgacagcta ccgctttaaa ttgatggcaa cattaaccga tgttgcagag aaaacggcga
     5881 ctgcctatat ggaagtcagt cgttatcagg ctttgtgcga tgcggctcaa cgcaatattc
     5941 actcgctgga aaacgtctac aacatggcgg cattgcgcgc taatgctggc ctgaactcat
     6001 cgtcggatga attacaagcc cagacgcgca ttgccggaat gcgctcaacg ctggagcaat
     6061 atcaggcgca gatggcaagc gccaaagcgc aactggcggt gctcactggc gtacagccgg
     6121 aggcgatagc cgcgccacct gctgaattag ccgagcagcc ggtatcgctg aaaaatattg
     6181 attaccagtc catcccgctg gtgctggcgg cagaaaactt acgccagtca gcacagtacg
     6241 gcgtggaaaa aacgaaagcg caatactggc caacgctcag tattcagggg ggcaaaacgc
     6301 gctaccagac cagtgaccgc tcgtattggg acgatcagtt acaactgaac gttaacgcgc
     6361 cgctgtatca gggcggcgca gtttcagccc aggtgcaaca ggctgaaggg caacaaaaaa
     6421 tctctgcctc gcaggtcgaa caggcaaaac tggatgtgct gcaacgagcg tctgtggcat
     6481 atgccaactg gacaggcgca cggggtcgcg aagaagccgg tttagcacaa tccgaaagtg
     6541 cgcacaaaac gcgagatgtg taccaaaatg aatataaatt gggcaaacgc agtctgaatg
     6601 atctattaac cgtcgagcaa gatgtctttc aggcgcaatc ggctgaaata aatgccaatt
     6661 atgatggctg ggtcgccgcc gtaaattatg ccgctgcggt gaataacctt attccattag
     6721 cgggaattaa acagggctta tacaacgact tacccgactt gaaataatcc tgccattatt
     6781 aatatcaggt atgcctcgtt gttaagtgat tacctttgtc ttatttagat aaatggactc
     6841 gctaaggata tgcgccattt atcaggaatg ccattatgag cctgattatt gatgttattt
     6901 cgcgtaaaac atccgtcaaa caaacgctga ttaatcctgg cgacgtcacg gttgttattt
     6961 atgagccttc cgtggtgcag gttcatgctc aggcctctgc cgtttcgcgt tacgtccgtg
     7021 aaggaaatga cctgctgatc tatatgcagg acggcacggt gatccgttgc aacggttatt
     7081 tcctgcaagc ggcgaatacc gctgaacaat cggaactggt gtttgccgat gggcaacagc
     7141 taacccatgt cacctttgcc gatactgctg cgggtggttt agcccccgta gaactgactg
     7201 cccagaccac tgcgattgaa agcattgcgc catttcttga taccgttgct cagaccagcg
     7261 ccttcccgtg gggttggctg gcgggggcgg cggtaggtgg tggtgcgctt ggtgcactgc
     7321 tggcaagcgg tggtgatggc gactcgaaaa cggaagtgat taataaccct acgccacccg
     7381 ctgagcctgg caacgccaca ccatcatttt tagttaccga taatcagggc gatcagcgcg
     7441 gcattctcgc caccaatgac atcaccgatg acaccacgcc aacctttagc ggcagcgggc
     7501 aggcgggggc gactattcag attaaagaca gtaacggcaa tactattgcc agtactcagg
     7561 tagacaacaa cggtcactgg agtgtctcgt tacccacgca aagtgcaggt gaacatacct
     7621 ggtcagtggt gcaaattgtc ggcagtacca tcactgacgc cggttcgata acgttaacca
     7681 tcgacaatag tcaggccagc gtgcaggttg ccaccaccgc aggcgataac attattaacg
     7741 ccagcgaaca ggccgccggg tttacgcttt ctggcaccag tagtcatctg gcacagggaa
     7801 cagaactcac cgttacgcta aacggcaaaa cctacaccac cagcgtaggc gctaacggtg
     7861 cctggagcgt gcaggtaccg accgccgatg cgcaggcgtt aggcgaaggg aatcaggcag
     7921 tgctggtcag cgggaaagac gccacaggca atacggtcac cggcgcgcag cttttaacgg
     7981 tcgataccca accgccaacg cttgccatca acactatcgc tcaggacaac atcgtcagtg
     8041 ctgcggaaca taacgccgcg ctggtactga gcggcacttc gaatgcagaa gcggggcaaa
     8101 ccgtaaccct gaccgtcaac gggaaaagcc attcagtaac cgtcggtagc gacggaacct
     8161 ggcaagtgac gctgcctgcc acggaggtac aggcactggc ggaggggaac tacgctgtca
     8221 atgccagtgt cagcgatcgg gcaggaaaca ccaccagcaa cagcgcgaat ttcacggtag
     8281 acacctcagc acccgtggtc agcgttaaca ccgtggcggg cgacgatatt cttaataatg
     8341 ccgagcaggc cgtcgcgcag atcatctccg gacaagtcag cggtgcttct cctggcgata
     8401 cggtaacggt gaaattgggc actcatgtcc tgacgggcat tgtgctggca gatggcagct
     8461 ggaatgtggc gctggacccg gcggtaaccc gcacgctgga tcgcggagcc aatacgattt
     8521 tcgtcaccgt gacagatact gcaggaaata ccggcgcggc gtctcgagca atcacgctgg
     8581 tcggtgtctc tccgttaatc accattaaca ccgtctccgg cgatgacatt atcagtggcg
     8641 cagaaaaagg tgcgccactg acccttaccg gtagcactca acaggccgag acaggacaaa
     8701 ccgtcacagt aaccctggct ggacagagtt ttactactac cgtgcaggct gatggctcct
     8761 ggagtctgac ggtacctgcc gccgcgatgg gaaatctgcc tgacggcgcg gtggcgatta
     8821 ccgcttctgt gacggatctc agcggcaata ccggcaacac ctcccgcacc attaccgtcg
     8881 acagccaggc cccgacgttg agcatcgatc cactgaccgc tgataacatc atcaacgccg
     8941 ccgaaagcgg gcaggatctt cccatcaccg gcaccactga cgcccagccg gggcagacgg
     9001 tgaccgttac gttgaatggg cagacctatc agggcgtcgt gcagtccgac ggcacctgga
     9061 gcgtgaccgt tcccgccacc aatgttgacg cactggctga cggcaacgcc acggtcaccg
     9121 ccagcgtgaa cgatgtcgcc ggtaacccga ccagcgtttc acgggtggcg ctggtcgatg
     9181 ccacgccgcc ggtggtgacc attaatccgg tggcgaccga taacgtcatc aacacgccgg
     9241 aacacaccca ggcgcaaatc atcagtggca cggttactgg cgcgcaggcg ggcgatattg
     9301 tcaccgttac gttaaataat tccgattaca ccacggtggt ggatggttcc ggcaactgga
     9361 gtctgggtgt tccggcttcc gttgtcagcg gactggtgga cggcagttat cctgtcagcg
     9421 tctcggtaac cgaccgcgca ggaaacagcg gcagccagtc attgaccgtc acggtcaata
     9481 ccgcagcgcc ccttatcggc attaacagca ttgcgggcga tgatgtgatc aacgccagcg
     9541 aaaaaggagc cgatctccag atcaccggca ccagcgatca gcctgttaat accgccatca
     9601 ccgtaacgct gaacgggcaa aattacacca ccacgaccga cgcctccggc aactggagcg
     9661 tcaccgttcc ggcatcggcg gtaaccgcac tgggccaggc caactacacg gtaacggcgg
     9721 cggtaaccag cagtatcggc aacagcaaca ccgccagcca taacgtgctg gttgacagcg
     9781 cgctgcccgg cgtgaccatt aacccggtgg caaccgacga tattattaac gccgccgaag
     9841 caggcgtcgc gcaaaccatc agcgggcagg ttactggcgc ggaagatggc gacacggtaa
     9901 ctattacgtt gggcggtaat acttatacgg cgacggtggg cagcaatctc acctggagcg
     9961 tgagcgttcc ggcggcagat attcaggcgc tgggtaatgg cgatttaacg gtgagcgcct
    10021 cggtcaccaa tcaaaacggc aacaccggca gcggctcgcg ggatatcacc atcgacgcca
    10081 atctgcccgg tctgcgggtc gatacggtgg cgggcgatga tgtggtcaat atcatcgagc
    10141 atgggcaggc gctggtgatt accggcagca gctcggggct ggctgaaggc acgctgctta
    10201 ccgttacgat taacaatgtg gaatacatca ctgcggtgca ggccgatggt agctggagcg
    10261 tgggcgtcac ggcggcgcag gttagcgcct ggcctgcggg gacggtgtct attgctgtat
    10321 ccggggaaag tagcgccgga aatccgataa gcattacgca tccggtaacg gtggatctca
    10381 ctccggcggc tatcaccatc aacactatcg ccacggacga tgtgattaac ggcgcagaaa
    10441 aaggcgctga tttaacgctt tccggcacca ccacgaatgt agaagccggg caaacggtca
    10501 ccgtcacctt tggcgggaaa aattacactg ccagcgtggc gagcgatggt agctggagtg
    10561 ccaccgtttc cgctgccgat ttagcgttat taaccgatgg cagcgcctcc gcgcaggcca
    10621 gcgtcagcaa tattaacggc aatagcgcct cggcggtgca taactacagc gtcgacagca
    10681 gtgccccgac catcattatc aataccgtcg ccagcgacaa tatcgtcaac gccagcgaag
    10741 ccgatgcggg tgtgacggtg agcggcagca ccaccgctga agcggggcag attgttacgg
    10801 taacgctcaa tagcccgacc gtgcagacct atcaggcaac ggtgcaggcg gacggcagct
    10861 ggagcatcaa tattccggcg gcagatcttg aggcattgac cgatggcagc cacaccctga
    10921 ccgccacggt caatgacaaa gcgggcaacc cggcgagcac cacgcataat ctggcggtgg
    10981 atctcaccgt tccggtactt accatcaaca ccattgcggg cgacgatatt attaacgccg
    11041 ccgaacacgg gcaggcactg gtgatttccg gttccagcac tggcggagaa gcgggggatg
    11101 tcgttagcgt cacgctaaac aataaaacct acaccacgac gctggatgct tccggcaact
    11161 ggagcgtcgg cgttccggcg gcggatgtca ccgcgctggg tagcggcccg caaaccgtga
    11221 ctgcgaccgt taccgatgcc gccggtaaca gcgatagtga gacgcacacg gtcaccgtta
    11281 accttaccgc accgaccatt ggcattaaca ccatcgccac cgatgacgtg attaacgcca
    11341 ccgaaaaagg cgcggatctg caaatttccg gcaccagcaa tcagccatcg ggcaccacaa
    11401 ttacggttac gctgaacggg caaaattaca ctgccaccac agatgcttcc ggcaactgga
    11461 gcaccacggt gcctgcttcc gctgtcggtg cgctgggcga agccagctac acggtgacgg
    11521 cgaacgtcac cgacagcgcg ggcaacagca attccgccag ccataacgtg caggtcaata
    11581 ccgcgctgcc tggcgtcacc cttaatccgg tggcgagcga cgatattatc aacgctgcgg
    11641 aatcgggcgt ggcgcagacc atcagcggcc aggtgacggg tgcggcggcg ggcgatacgg
    11701 ttaccgtaac gctaggcggg aaaacttaca ccgctaccgt gcaggggaat ttaagctgga
    11761 gcgtggacgt tccggcggcg gatattcagg ccatcggtaa tggcgatctc acggttaatg
    11821 cctcggtaac caacggtgtt ggcaatactg gcagcggttc gcgcgatatt gtgatagacg
    11881 ccaacctgcc gggtctgcgc gtcgataccg tggcgggcga tgatgtggtt aacagcatcg
    11941 aacacggtca ggcgctggtg ataaccggta gcagcagcgg gctggcggcg ggcgcggcgc
    12001 tgacggtggt gatcaataac gtcacctatg gcgcgacagt attagccgat ggcacatgga
    12061 gccttggcgt tccggcagca gacgtgggca actggcctgc gggtacagtg aatatcattg
    12121 taagcggcac taacactgca gaaacgacta cgactatcac ccatccggtc accgtcgatc
    12181 tggcggcggt ggcgatctcc attaacaccg tttccggcga cgatgtgatt aacgccgccg
    12241 aaaaaggggc cgatttaacc ctttccggca gcacctccgg cgtggagctg gggcaaacgg
    12301 tcaccgttac ctttggcggc aaaacctaca ccgccaccgt agcgggcgat ggtagctgga
    12361 caaccatcgt gcctgccgcc gatctcagcg cgttacgcga cggcgacgcc tcggtgcagg
    12421 ccagcgtcag taacgttaac ggcaacacgg cttcggcgac ccacgcctac agcgtcgatg
    12481 ccacggcacc gacgcttgcc attaacacca tcgccactga cgatattctg aacgccgccg
    12541 aggcgggcaa tccgctaacc atcagcggca gtagcaccgc cgaagcgggg cagacggtaa
    12601 ctgtcacgct taatggcgtg acttacaccg gcaccgtcct ggcggacggg agctggagcg
    12661 tcagcgtgcc gacggcggat ctcagcaatc tgaccgccag ccagtacacg gtcagcgcct
    12721 cggtaagcga taaagcgggt aacccggcaa ccgccactca tgggctggcg gtggatctca
    12781 ccgttcccgt attaaccatc aacaccgtct ccggcgatga cattattaac gccgccgaac
    12841 acgggcaggc gctggtaatc tccggttcca gcgctggcgg cgaagcgggc gatgtcatca
    12901 ccgttacgtt aaacagtaaa acctatacca ccacgctgga cgcttccggc aactggagcg
    12961 tcggcgttcc gctgtcagac gttaccgcgc tgggcagcgg cccgcaaacc atcacagcta
    13021 ccattaccga cgcggcaggc aacagcgatg atgccagccg cacggtcacc gtgaatctca
    13081 ccgcgccaac cattggcatc aacactatcg ccagcgatga cgtgattaac gccacggaaa
    13141 aaggcgcgga tctgcaaatt accggcacca gtaatcaacc cgcaggcacc acgataacgg
    13201 tgacgctgaa cgggcaaaat tacaccgcta ccacagatgc ttccggcaac tggagtgcca
    13261 cggtgccagc ctcggctgtc agtgcattgg gtgaagccaa ctacacagta accgcgaacg
    13321 tcaccgatac ggcaggcaac agcaactccg ccagccataa cgtgctggtc aacagcgcct
    13381 tgcctgccgt caccattaac gcggtggcga ccgacgatat tattaacgct gctgaatccg
    13441 gaaacgcgca aaccatcagc ggccaggtga cgggcgcggc ggcgggcgat acggttactg
    13501 ttacgctggg tggcaacacc tacaccgcca cggtgcaggc taatttaagc tggagtgtca
    13561 gcgttccggc ggcggatatt caggccatcg gcaacggctc tctcaccgta aacgcctcgg
    13621 tgaccaacgt cgtcggcaat accggcaacg gctcgcgcga tatcacgatc gacgccaatc
    13681 tgcctggcct gcgggtcgat accgtggcgg gcgatgatgt tattaacagc attgagcaca
    13741 atcaggcgct ggtgatcacc ggcagcagca gcggattaac ggcgggaacg gcgttaacgg
    13801 tcgagattaa caacgttact tatggcgcga cggtattagc cgacggcacg tggagcctcg
    13861 gtattccggc ggcagatgtc agcaactggc ctgcgggtac ggtggatatt acggtaagcg
    13921 gcaccaacag cgccggaaca acctctacca ttacccatcc ggttaccgtc gatctggcgg
    13981 cggttgccat caccattaac accctttccg gcgatgatgt gatcaacgcc gtggagaaag
    14041 gcgaaacgct ggtggtgagc ggcagcacca gcggtgtcga agccgggcag acagtgaccg
    14101 tcacctttgg cggcaaaagt tacaccacca ccgttgaagc caacggcagc tggacggtga
    14161 atgtgccgcc tgccgatctt gcttcgctgc cggacggcgc gggcaacgtg caggcgagcg
    14221 tcagtaatat taacggcaac agcgcccagg cggatcgcgc gtatagcgtt gatgccaccg
    14281 cgccgcttgt gaccatcaac accatcgcca gcgacgatat ccttaacgtg agcgaagcgg
    14341 gcgcagggat caccatcagc ggcaccacca cggcacaggt cgggcagacg ctgaccgtca
    14401 cgctgaataa caacacttac cagaccaccg ttcaggcgga tggcacctgg agcgtgaatg
    14461 ttccggcaac ggatttaagc ggattaaccg ccagcagtta taccgtgact gcgacggtga
    14521 gcgacaaagc gggcaacccg gcaagcgccg atcacgcgct ggcggtggac gttaccgcgc
    14581 cggatctcac cattaacacc gtcgcgggcg atgacattat caacgccatc gaacacgggc
    14641 aggcgctggt ggtcagcggc accagcacgg gcgcggcggc gggtgatgtg gtcacggtta
    14701 acctgaacgg taaaaactac accaccacgc tggacgcctc cggtaactgg agcgtgggta
    14761 ttccggcggc ggatgtcacg gcgctgtcaa ccggtagcca gacgatcacc gccagcctga
    14821 gcgatcgcgc gggcaacagc gacagcacga ctcacgatgt gaccgttgat ctcagcggcc
    14881 cgacgctgag catcaatacc gtctccggcg atgatattat taataatgcg gagaaaactc
    14941 aggatctgac catcagcggc ggctccagcg gtctggcgac tggcaccacg gtcaccgtga
    15001 tgttgaacgg cctggcgtac agcgccacca ccgacggtac cggcaactgg agcgttaccg
    15061 tgcccgcttc ggcggttggc gcactgggcg aggcggtata ccagattagc gccagcgcca
    15121 ccgacagcgc gggcaacagc ggcagcacca cccatacggt gaaggtcgaa tcgctcctgc
    15181 ctggggtaat cattaatacc gtggcgggcg atgacattat caacgctgct gaaatcgccg
    15241 ttaatcagac cattagcggc caggtctcgg gaacggcggt tgcggggaat acggtgattg
    15301 tcaccattgg cggcaatcaa tacaccgcca tcgtgcagtc agatttaagc tggagcgtca
    15361 gcgtacctgc gaacgttttg caggcgctgg gcaacggtga actgaccatc agcgcctcgg
    15421 tgactaatgg cgtgggtaat actggcaccg caacgcacga catcgtgata gacgccaacc
    15481 tgccaggtct gcgggtcgat accgtggcag gcgatgatgt gattaacagc attgagcaca
    15541 ctcaggcgct ggtggtcacc ggtagcagta gcggactggc agcaggcgcg gcgttgacgg
    15601 tagtgatcaa cggcgtcacc tatggcgcaa cggtactcgt cgatggcacc tggagcgtcg
    15661 gtgttccggc ggtagatgtc acaaactggc ctgcggggac ggtcaatatt gccgtctctg
    15721 gcactaacac tgccggaacc acaaccagca ttactcatcc ggtcacggtc gatctcgcgg
    15781 ccgtggcgat caccatcaac cccctttcca ctgacgatgt gattaacgcc gccgaaaaag
    15841 gcagcgattt gcagctctcc ggcaccacct ccggcgtgga agcggggcag accatcaccg
    15901 ttatcttcgg tggcaaaagc tacaccacca cggttgccgc ggataatagc tgggggctga
    15961 cgatcccagc cgccgatctc gcaaccttgc cagacggcgc agcaaacgtc caggccagcg
    16021 tcagcaatgt cgctggcaac agcgcccagg caacgcatgc atacagcgtt gatgccactg
    16081 caccctccgt caccatcaac accatcgcca cggacgatat tcttaacgcc gccgaagcgg
    16141 gatcggcgct gaccatcagc ggcaccagca cggcagaagc cgggcagacg gtgaccgtga
    16201 cacttaacgg cgttaattac agcggcaacg tgcaggcaga cggaagctgg agcgtcagcg
    16261 tcccgaccgg cgatttagct aacctcactg ccagccccta tacggtgagc gcagcggtca
    16321 gcgacaaagc cggaaatccg gcttcagcaa cacataatct gacggtggac cttgccgctc
    16381 cggttgtcac cattaacacg gtggcgggcg atgacatcat caacgccacg gaacattctc
    16441 aggcgcagat tatcagcggc tcggcaacgg gcgcgacgac cggtaacacg gtttccgtga
    16501 ccattggcac gactacctat accaccgtgc tggacgccaa cggcaactgg agcatcggtg
    16561 tgcctgccag cgtgatttcc gcactggcgc agggggatgt gaccattacc gccacggtca
    16621 ccgactccgc aggcaacagc ggcacggcct cgcacagtgt cagcgtggcg ctcggcgctc
    16681 cggtacttag cattaacacc attgccgtcg acgacatcat taatgccacg gagaaaagcg
    16741 cggatctggc gatttccggc accagcgacc agcctgcggg cacgcagata accgttacgc
    16801 tcaacgggca aaattacacc accactgccg atgcatccgg taactggagc gtgaccgttc
    16861 cggcgtcacg ggtgagcgcc ctcggtgaag ccacttacac ggtgaccgcc gccgctactg
    16921 actctgatgg caacagcggt tccgccaacc ataacgtgca ggttaatacc gcgctgccgg
    16981 gcgtcaccat taacgttgtc gcgagcgacg atattattaa cgctgccgaa gcgggcgcgg
    17041 gccagagcat cagcgggcag gtgacgggcg cggcagcggg cgacacggta accgtcacgc
    17101 tcggcggggc gacgtacact gccacggtgc aggcgaattt aagctggagt attaatgttc
    17161 cggcctccgc gctacaggcg ttgggcaacg gtgagctgac catttcggct tcggtaacga
    17221 acagcgtagg caataccggc aacggcacgc gcgaaatcac catagacgcg aatctccccg
    17281 gtctgcgggt cgataccgtg gcgggcgatg atgtggtcaa tatcatcgag cacggacagg
    17341 cgctggtgat taccggcagc agttccggtc tggcgacggg cagtaacgtc acgctgacca
    17401 ttaacgggca aacctatgtt gcggcggtgc tggcggatgg ctcatggagc gtcggcgttc
    17461 cggcggtgga tgtcagcgcc tggcctgcgg ggacggtcac gattacggcg agcggtagca
    17521 ccacagccgg aaattcggtc agcgttacgc atccggtgac tgtcgatctc tcggcgattg
    17581 ccgtcagcat caacgccatt accgccgatg atgtgattaa cgctgctgaa aaaggcgcgg
    17641 cgttaacgct caccggcagc acttctggtg tcgaagccgg acaaacggtc accgtgacct
    17701 ttggcggcaa aacttacact gcaagcgtcg cggctaacgg ttcctggagt accacggttc
    17761 cggcggcaga tatggcagcc ctgcgtgatg gcgatgccag cgcacaggcc agcgtcagca
    17821 atgttaacgg caacaacgcc actactaccc acgcttacag cgtcgatgcc agcgcgccaa
    17881 cggtggccat taacactatt gcaggcgacg atattcttaa cgccgccgaa gcgggtgctg
    17941 cgctgactat cactggcagc agcacggcgg aagcggggca gacggtgacc gtcaccctca
    18001 atggcgaaaa ctatactggc acggttcaaa cggacggtag ctggagcgtc agcgtaccgc
    18061 cagccgacgt aagcgccctg accgccagca actacaccgt tagcgcggcg gtgagcgaca
    18121 aagccggaaa cccggcctcg gttaaccaca acctgacggt ggatacttcc gttccggttg
    18181 tcaccatcaa cacggtggcg ggcgatgatg tgatcaacgc gacggaacac gcccaggcgc
    18241 agattatcag cggctccgcc acgggtgcgg caaccggcag caccgtgacg gtgactatcg
    18301 gcagcagcac cttcaccacg gtgctggatg ccagcggtaa ctggagcgtc ggcgttccgg
    18361 caagcgtcgt ctcggcgctg gcgaacggca cggtgacaat taacgccagc gtcaccgatg
    18421 ccgccggaaa cagcggcagc gctacgcatc tggtgacggt caatactggc cttccgtcaa
    18481 ttacctttaa cgccatcagc ggcgataacg tcctgaacgc cgatgaaaaa ggccagccgt
    18541 tgaccattag cggtagcagt acggggttgg cgaccggcgc acaggttacc gtcacactca
    18601 acggtcataa ctacagcgcc accaccgacg cggcgggcaa ctggacgtta accgtgccgg
    18661 tgagcgatct ggcggcttta ggccaggcca actatatcgt cagcgccagc gccaccagtg
    18721 tggcaggcaa caccgccagc agccaggcga atttactggt cgatagcggc ctgccgggtg
    18781 tcaccatcga caccgtggca ggcgacgata ttatcaacgc cgccgaagcc ggggacgatc
    18841 aaaccatcag cggggtggtg actcgcgccg ccgctggcga tacggttacc gtcacgctgg
    18901 gcggcaacac ttacaccacc acggtacaga gcaacttaag ctggagcgtc accgttccgg
    18961 cagccgatct ccaggcattg ggcaatggtg atttgaccat tacagcctca gttactaacg
    19021 cgaatggcaa taccggcagc ggcacgcggg atatcaccat tgacgctaac ctgccagggc
    19081 tgcgcgtgga tacggtggcg ggcgatgata ttgtcaacag cattgaacac gggcaggcgc
    19141 tggtaatcac cggcggcagt agcggcctga atgcaggcgc agcgctgacg gtcgccatca
    19201 acggtgtggc ctatgccgcc acggtacagg cagacgggag ctggagcgtt ggcattccgg
    19261 cggcaagcgt cagtgcatgg cctgcggggc cgttaaccgt tgaagttaca ggacagagca
    19321 gcgccggaaa tccggtcagc gccagccatc cgttcaccgt cgatttaacg gcggtggcaa
    19381 tcagcatcaa caccgttgcc agcgacgacg taattaacgc cgccgaaaaa ggcactggtc
    19441 tgaccctttc cggcagcacc agcgggattg aaagcgggca aaccgttacc gtcatctttg
    19501 gcggtaaaac ctacaccgca agcgtcgccg ccaacggaag ctggagtgtg aacgttccgg
    19561 cggcagatct ggcaactctg ccagacggcg cggcgaacgt gcaggccagc gttagcagcg
    19621 cgagcggtaa cagcgcctcg gcgacccatg cgtacagcgt tgacgccagc gcgccgacgc
    19681 tcaccattaa caccattgcc agcgacgata ttcttaacgc cacggaggcg ggaagcccgc
    19741 tcaccatcag cggcaccagc accgccgaaa ccgggcagac ggtcaccgtc acccttaacg
    19801 gcgcaaccta cagcggtaat gtgcaggcag acggtagctg gagcgtcagc gttccccctt
    19861 cagccttagg cgcgctgagc gcaagcaatt acaccgtcag cgccacggtc aacgacaaag
    19921 cgggcaaccc cggcagcgcc agccataatc tggcggtaga caccaccgcg tcggttctca
    19981 ccattaacac tgtggcgggc gatgacatta tcaacgatgc cgaacatgcg caggcgctgg
    20041 tgatctccgg caccagcacg ggcggggaag cgggcgatgt ggtgagcgtt gtgctcaacg
    20101 gcaaaaccta caccaccacc ctggatgcct ccggcaactg gagcgttggc gttccagcgg
    20161 cggatgttgc ggcactcggt agcggtgcgc agaccatcac cgccagcgtc agcgatcggg
    20221 caggcaacag cgacgacgcc agccgcaccg tgaccgtcag cctcagcgcg ccagtgatta
    20281 gcatcaacac catcgcgggc gatgatgtga tcaacgcgat ggaaaaagga tccgatctgg
    20341 cgctttccgg caccagcgat cagcctgcgg gtacggcgat caccgttacg ctgaacggac
    20401 aaaactacag cgccaccact gacgcctccg gcaactggag cgttaccgtg cccgcctcgg
    20461 cggtcagtgc gctgggcgaa gccacctaca gcgtcacggc gagcgtcacc aatgctcagg
    20521 gtaacagcag caccgccagc cataacgtgc aggtcaatac cgcgctaccg ggcgtcacca
    20581 ttaatccggt aacgacggac gatattatta acgccgcaga agcaggcagc gcgcaaacta
    20641 tcagcggcca ggtaaccggt gcggcggcgg gcagcaccgt caccgttgaa ctgggcggta
    20701 aaacttacac cgccaccgtc caggctgatt taagttggaa tgtgagcgta cctgccgccg
    20761 actggcaggc gctgggtaac ggtgagttaa cggttaatgc ctcggtgacc aacgccgttg
    20821 gcaacaccgg tagcggcacg cgcgatatca ccatcgatgc cagtctgcca ggtctgcggg
    20881 tggataccgt tgcgggcgat gatgtggtca atatcatcga acacgctcag gcacaggtga
    20941 tcaccggcag cagctccggc tttgccgcag gtacggcgtt gaccgtagtg ataaataatc
    21001 aaacttacgc cgccacggtg ctggcgaacg gtagctggag cgttggcgtt cctgcgacgg
    21061 atgtcagtaa ctggcctgcg ggaacgctga atattaccgt tagcggcgcg aacagtgccg
    21121 gaacgcaaac cagcattacc catccgctga ccgttgatct caccaccgtc gccgtcagca
    21181 taaacagcat caccagcgat gatgtgatta acgccgccga aaaaggcgcg gcgctaacgc
    21241 tctccggcag cacgtccggc gtcgaagcgg ggcaaaccgt taccattact tttggcggta
    21301 aaacctacac caccacggtg gcggcaaacg gtagctggag caccaccgtt ccggcggcgg
    21361 atatggcggc cctgcgtgac ggcgatgcca gcgcccaggt gcgggtgact aacgtcaacg
    21421 gcaacagcgc cacggcaacg catgaataca gcgtcgatag cgccgcgcca acggtcacca
    21481 tcaacaccat tgccagcgat aacatcatca atgccagcga agcggcggcg ggcgtaacgg
    21541 tgtccggcac cagcacggcg caaaccgggc agacgctcac cgtcacgctt aatggcacta
    21601 actaccagac cacggtgaag gcagacggca gctggagctt aacgctgccc gccagcgact
    21661 taaccgcgct tgccaataac ggctacaccc tgaccgccac ggtcagcgat caggcgggta
    21721 accctggcag cgccagcaaa ggtgtgaccg tcgataccac cgcgcccgtc atcagtttca
    21781 acaccgtggc gggcgatgat gtgattaaca acgtcgaaca cactcaggcg cagattatca
    21841 gtggcaccgc cacgggcgcg gtggcgggcg accgcctggt ggtgaccatc gccgggcagc
    21901 agtatgtcac cagcaccgat gccagcggca actggagcgt tggcgtgcct gccagcgtga
    21961 tttccggcct tgcggacggc acggtgacca tcagcgccac cattaccgat agcgcaggca
    22021 acagcagcac gcagacgcac aacgtgcagg taaacacggc ggcagtgtcg ctctcggtca
    22081 gcaccatcag cggcgataat attattaacg ccgccgaagc gggcagtgcg ctgaccctga
    22141 tcggcacagg aacgaacttt gccgcaggta cagtagtgac cgtattgctt aacggcaaag
    22201 gctacagcgc taccattcag aacaacggga gctggagtgt gaacgtgcct gcggcggacg
    22261 ttgcagcact ggcggatggc accagctaca cggttagcgc ctccgcgcag gacagcgccg
    22321 gaaacagcgc cacggcctcg cgcagcgtgg cggtggatct caccgcgccg gttatcagca
    22381 tcaacaccgt ttcgacggac gaccgactca acgccgccga acaacagcag ccgttaaccc
    22441 ttaacggctc gaccagcgcg gaagtagggc agacggtcac cgtcaccttt ggcgacaaaa
    22501 cctataccgc cacggttgcc gccaatggca cctgggcgct taacgtgcct gcggcggatc
    22561 ttgccgcgct cgggcagggc gcgcagacta ttaccgccag cgtgaacgat cgcgcgggca
    22621 atcccggtca aaccacgcac gccctaaccg tcgataccgt tgcgcccacg gtgaccatcg
    22681 ccacggtggc gggtgacgat attatcaaca acgccgagca gcttgccggg cagaccatca
    22741 gtggcaccac caccgctgaa gtgggccaga cggtgaccgt cacctttaac ggtcagagct
    22801 ggactgcaac ggtcggcagc ggcggaagct ggtcggtgtt tattccggcg cagcagtttg
    22861 ccggattaag cgacggcagc tacaccatta gcgcgacggt tagcgatcag gccggaaacc
    22921 ccggcagcgc cagccgtggc gtgacgctga acggcgacgt cccctccgtc accatcaaca
    22981 cctttgcggg cgatgatgtg gtgaatgcag cggagcacgg ctcgtcgctg gtgatcagcg
    23041 gcaccaccac cgcacccatc gggcagacgc tgaccctgac tttaaacggc aaaacctaca
    23101 ccactacggt gcaaacgggc ggtagctgga gctacaccct cggcagcgcc gatgtcaccg
    23161 cgctggcgga cggcaatgcc tatgtgatta acgcctcggt gagcaacgcc attggcaata
    23221 ccggtagcag caatcacacc attactgtcg atctcagcgc cccggcgatg ggcattaata
    23281 tcgattccct gcaagccgac actgggctta gcgccagcga ctttatcacc agcgtcagtc
    23341 cggtggtggt caacggctcg ctcaccgccg cgcttgccag taacgagacg gcgcaaatca
    23401 gtatcgacgg cggcgtaacc tggaccacgc tcaccgttac cggcacgacc tggcgctata
    23461 acgacagccg cacgctgacc gatggcaact atctctatca ggtgcgggtg attgacgcag
    23521 cgggcaacgt cggtgccacc gacagccaga atgtggtgat cgacaccacc gcgccagacc
    23581 cagcggtgaa aaccatcgcc attagcgcga tcaccaccga tacgggcttg atcactaacg
    23641 attttgtcac cagcgacacg acgcttgcgg tgagcggcac gctgggcgcg gcgctttctg
    23701 ctggtgagtt cgcgcaaatc agtatcgacg gcggcaccac ctggcagaac ctgtcagtaa
    23761 gcggcttaac ctggacctgg cttgacgggc ggacgctgac cgatggcaac tacaactatc
    23821 aggtgcgggt tatcgacacc gccggaaata tcggcgcgac cgccagccag atagtcactg
    23881 tggacaccac cgcgccgctg gcgagcaaaa ccatcgccat tgctggcatc agcgatgaca
    23941 ccgggttaag tagcagcgat tttgttactc gcgacaccac gctgaccgtg cgcggcacgc
    24001 tcggcgcggc gcttgccgcc gatgaacggg cgcaaatcag cctcgacggc ggtgtcacct
    24061 ggaccacgtt aaccgttatc ggcaccagct ggagctatgc cgatggtcgc acactgaccg
    24121 acggcacctg gaactacacg gtgcgggtgg tggatctggc ggggaacgtt gggcagaccg
    24181 caacgcaaaa tgtggtggtc gacaccacca gcccggaagc ggcgaaaagt atcaccatta
    24241 ccggtatcag cgatgacacc ggagccagca gcagcgattt tattaccagc gacacctcgc
    24301 ttaccgtgcg cggcgtattg ggggcggcgc tcggcgctaa cgagttcgcg caaatcagta
    24361 ccgacaacgg cgcaacctgg gtgaacgtga ccgtcgccgc agacggcctt aactggagtt
    24421 acgttgacgg acgcacgctc accaacggca ccaccacctg gcaggtgcgg gtggtcgatc
    24481 tggcgggcaa cgttggcgca accggcagcc agtcggcgca gattgacacc gtgaacccgg
    24541 cgcaggtgct caccatcgcc agcatcagca ccgacacggg gagttcggca actgacttta
    24601 tcaccagcga tacctcgctc acgctgaccg gttcgctggg ggcggggctt gccagcggcg
    24661 aagtggcgca gattagcctc gacggcggcg cgacctggat cacgctcacc accaacggca
    24721 cgcagtggac ctacaccgac ggccgcacgc tgaccgacgg cagctacgtt tatcaggtgc
    24781 gggtgctgga tctggcggga aataccggtc cggtggtgtc gaaaacggtg gtggtcgata
    24841 cgattaaccc caccgcgacg ccgaccattg tgtcgtacac tgatgacgtc gggcagcggc
    24901 aggggacatt aagcagttcg caggccaccg acgacactac gccgttgctg aacggcgtgc
    24961 tctccggccc gcttgccagc ggtgaagtgg tttacctcta ccgtaacggc ctgctgttag
    25021 gcgcggtaac gatggtaggc gcactgaact ggacctacag cgacagcggg ctggtgagcg
    25081 gtgcctacac ctacagcgcg cgagtggtgg atctggcggg gaatatcacc tcctccagtg
    25141 attttgtcct gacggtcgat acctctattc cgaccacgct ggcgcaaatt accagccaga
    25201 ccacgcgcga taccacgccg attatcagcg gggtgatcac ggcggcactc gccagcgggc
    25261 agtatgttga agtggtgatc aatggcaaaa cctacacctc tgaaccgggc ggtgcggtgg
    25321 tagtcgatcc ggcgcacaac acctggtatg tgcaactgcc ggataccgat gcgctgacag
    25381 tttccgcgac cgcctatacc gttactgcgc aggtaaaaag ctcggcgggc aacggcaata
    25441 acgccaatat tagcaacggc acggtgacgg ttaacgcggc gattgattac acaccgacct
    25501 ggaccaccac cagcaaaacc accgcctggg ggctgaccta cggcctcgac tcgcacggga
    25561 tgtggacggt gctggcaaac cagcaggtaa tgcaatcgac tgacccactc acctggtcga
    25621 agaccgcgct gacgctgtat cagagcggca acaactacgc caccagctcc atcgccgatt
    25681 acgaccgtaa cggtacgggc gatctgttta ttacccgtga tgactacggt acggggtata
    25741 tcaacggctt taccaataac ggcgacggca ccttttccag cgctattcag gtcaccgtcg
    25801 gcacgctgac gtggtacggc tcgattgtgg catttgataa agagggcgac ggctatctcg
    25861 acttttggat tggcgacgcg ggcgggccgg actccaacac cttcctgtgg aacaacgcag
    25921 gtacgctggt aggcaactcc accacgtcga acagcggcgg tagcgctacg gtgggcgggg
    25981 cggtgacggg gtatctttcg cttaacgaag gttccggcgt cgatctgaat aatgacggca
    26041 ggatcgacct ggttcagcac acctacaacc tgaacaacta ttacacgcta tcttcgctca
    26101 tcaaccaggg gaatggcacg tttgtctggg ggcagaacac caccaatacc ttcctgagcg
    26161 gggcgggcag tggcgctatg agcagcagcg tttccatgac ctgggcagat ttcgatggtg
    26221 acggcgatat ggatctcttc ctgcctgcca gccagggaag agctaactac ggctccctgt
    26281 tattcaacac caacggcgtg ctgggcagtc cggtggcggt gggggcaacg gcaaccacct
    26341 atgccagcca gtttagcgtg gcggtggact ggaaccatga cggtctgatg gatatcgccc
    26401 gtatcgccca gaccggacaa tcgtatcttt ataccaacgt cagcaacgcc agcaactgga
    26461 cacaatcggc cctcggaagc agtcagagcg gcactaccag cggcgtggcg gcaatggact
    26521 acgactggga cggcgcggtg gatgtgctgg tgactaaaca gtcgggcagc gtctatctta
    26581 ttcgcaacac caacacggtg agctacggca cctcgctgca cctgcgcatc accgatccta
    26641 acggcattaa cgtctattac ggcaataccg taaaactgta caactcggcg ggagtgctgg
    26701 tcgccacgca aatcatcaac ccgcagtcgg gtatgggcgt caacgacacc tcggcgctgg
    26761 tcaatttcta cgggctgaac gccggagaaa cttacaacgc cgtgctgatc aaatccaccg
    26821 gcaccaccgc cagcaatatc gaccagacgg tcaacaccag ctggggcggc ttacaggcca
    26881 ccgatgccac ccacgcttac gacctcagcg ctgaagcggg caccgcgagc aacaacggca
    26941 agttcgtcgg cacgggctat aacgacacct tctttgccac tgcaggcacc gacacttacg
    27001 acggttccgg cggctgggtg tacagctccg gcaccggaac gtggcttgcg aacggcggca
    27061 tggacgtggt tgatttccgc ctctcgacgg tgggtgtgac cgcaaattta agcgtcacca
    27121 ccgcgcaggc taccggtttt aacacctcga catttaccaa tatcgaaggg atatccggct
    27181 cgaattttaa cgacactctg actggcagca gcggcgataa ccaactggaa gggcgcggcg
    27241 gcaacgacac ccttaacatc ggcaacggcg gtcacgacac cttgctctat aaactgctca
    27301 acgccagcga cgccaccggc ggcaacggct cagacgtggt gaatggcttt acggtaggga
    27361 catgggaagg caccgccgat accgatcgca ttgatattcg cgagctgttg cagggcagtg
    27421 gctacaccgg caacggcaaa gccagctacg tcaacggcgt agcaacgctg gatgcgcagg
    27481 ccggaaatat cggtgatttt gtcaaagtca cccagagcgg cagcgacacc atcgtgcaga
    27541 tcgaccgcga cggcacgggc ggcacttttg cgacaactaa cgtggtcacg ctgacgggcg
    27601 tgcataccga ccttgccact ttgctggcga atcatcagtt gatgttggtg tagagaatga
    27661 aaaaaaatgc ccagtccgtg gaagcctggc tggaggcgat gatcgccgtg gcgcgttatt
    27721 accgcctcga tttttctcag gagaacgtgc gcgcaaccgt caactgggag cgcgacagca
    27781 agcgggaaga gttgctcacc gatatggcgc ggcagttggg aatggggtta cggctggtgg
    27841 agttttccgc cgattcgctt aacccgtggc gactgccgtt gatcgccgtg ttcgataatc
    27901 agcagattgg cgtgatcacc cgccgcgata accacgacaa tatcagcgtt cagttcagcg
    27961 gtgacgaagg gctggaaacc acgcttaacg tcgcggatat tgaagataaa atcgtcgaac
    28021 tggcgctact gcgcccgctc agcgccatcc ccgacgcgcg cgtggatgac tatattcgcc
    28081 cgtatcaggc gaactggttc tggaatctgt cgctgaaaga ctggcggcgt tatggcgata
    28141 ttatgctggc ctcactggtg gcgaacgtgc tggcgctggc ggcgatgatt ttctcgatgc
    28201 aggtttacga cagggtggtt ccggcgcagt cctacccgac gctgtgggtg ctgttcgccg
    28261 gggtgatgat ggcgatcctg tttgaattct gtatgcgcat ggtgcgcacg cacctttccg
    28321 atgtgatcgg caagcgggcg gatctgcgca tttcggatcg cgtttttggt cacgcattac
    28381 ggctgaaaaa taacgtgcga tcgaaatcca ccggatcgtt tatctcgcag atccgcgaac
    28441 tggaatcggt gcgggagctg atcacctcca ccaccattgg tgcagtggcg gatctgccgt
    28501 tcttcctgct gtttgtcttt attttgtgga tgattggtgg ctggctggtg ctggtggttt
    28561 tgctggcgct gcccttgctg gtgatccccg gcctgctggt acaacgcccg ctggcgcggc
    28621 tggcgaacga agggatgcgc gagtcggcgg tgcgtaacgc tacgctggtg gaagccgtgc
    28681 agtcgattga agacattaaa ctgctgcgcg ctgaacagcg ttttcagaat cagtggaatc
    28741 acactaacga cgtggcctcg tccatcagca tgaagcaacg ttttctcacc gggctactgc
    28801 tcacctggac ccaggaagtg cagtccatcg tctacgtggt ggtgctgttg gtgggctgct
    28861 ttatggtaat gaacggcgac atgaccaccg gcgcgctggt cgggacgacg atcctggctt
    28921 cgcgcaccat cgcgccgctg tcgcagatat ccggcgtgct ctcgcgctgg cagcaggcga
    28981 aagtagcgcg taatggcctt gatgaactga tgaagcgccc ggtggatcag ccggaacacg
    29041 gcaaactggt gcataaagcg gtgctgcacg gcaattatca gttcagcaac gctatctttt
    29101 attacgacga agaagagaaa attgccgatg tggcgattgg caaactcaac attcaggccg
    29161 gagagaaaat cgccattctt gggcgcaacg gcgcgggtaa atcaacgcta ctgcaaatgc
    29221 tcgccggaat gcgtatcgcc cagcaggggc aggtgctgct ggataacatc agcatcggtc
    29281 agcttgatcc ggcggatctg cgccgcgaca tggggctgct gagccagacc gggagactct
    29341 ttttcggttc gctgcgggaa aacctgacga tggggatgcc ggaagccagc gatgaagata
    29401 tcgaacgcgc gttaaccctc agcggggcgc tgccctttgt gcagaagcag aaaaacggcc
    29461 tcaactacat gattcaggaa gggggatttg gcctctctgg tggtcagcgg caaacgctgc
    29521 tgctggcgcg gctactcatc tctcaaccca atatcgtgct gctcgatgaa cccagcgcct
    29581 cgctggacga aatggcggaa gcgtatttaa tcgaacaact gaaacagtgg attgggcatc
    29641 gcacgctggt catcgccact caccgcacgg cgatgctgca actggtggac cgcattatcg
    29701 tgatggatca ggggcggatt gtgatggatg gcgcgaaaga ggccattttg cgtgaacaga
    29761 gcgaacccac gacgcgacgg gtggtgttgc aggagaagaa taaggggtct gccgcatgaa
    29821 tggacaactg caatacaacc gtggcctgac tgaaccccgc ctgccgcgtt cggcgctggc
    29881 ggtacgcgtt accgctgtga tgctgctctg ttttctcggc tgggcgtggt acttccagct
    29941 tgatgaagtc accaccggca gcggcacggt agaaccttcc gggcgcgaac aggtggtgca
    30001 gtcgctggaa ggcggaattc tgtatcatct cgacgtcaag gttggcgata ttgttgaaca
    30061 gggccagccg ctggcgcagc ttaaccgcac caaaacggaa tccgacgtcc aggaagcaat
    30121 gtcgcggctg tacgcggcgc tggcaacctc ggcgcggctg cgggcggaag tgagtaataa
    30181 accgctggtc ttccctgacg aactgaataa attccccgaa cttatcgagt cggaaacggc
    30241 gttgtacaac actcgccgcg acgggttgaa caaagccacc accgggctga cgcaggggat
    30301 tagtctggtc aaccgtgaac tggcgatgac ccagccactg gtcaaacagg gggcggcgag
    30361 cagcgtcgaa gtgctgcgcc tgcaacgtca ggccaatgaa ctggaaaaca aactcagcga
    30421 cgtgcgcacg caatactacg tacaggcgcg cgaagagtta gcgaaagcca atgcggaagt
    30481 agaaacccag cgttcggtga tccgtggacg ggaagactcc ctcaccaggc tcaactttac
    30541 cgcgccggtg cgaggcattg ttcaggacat tgacgtcacc acggttggcg gcgttattgc
    30601 ccccggtggt aaactgatga ccatcgtgcc gctggatgag caactgctga tcgaggcaaa
    30661 aatctccccg cgcgatgtgg cgtttatcca tccggggcaa aaatcgctgg tgaaaataac
    30721 cgcctacgat tactccatct acgccggcct gccgggagaa gtggcggtga tatcaccaga
    30781 caccgtgcag gatgaagtgc ggcgcgatgt ttattactat cgggtgtaca ttcgcacttt
    30841 cagcaaccat ctggaaaata aacataaaca gcagtttccg atatttcccg gcatggtggc
    30901 gacggtcgat atccgcaccg gcaaaaaaag cgtgctggat tatctgttga gaccctttaa
    30961 taaagcacag gaggcgttgc gcgaacgatg aacattagcg atgtagcaaa aattaccggc
    31021 ctgaccagca aagccattcg cttctatgaa gagaaggggc tggtgacgcc gccgatgcgc
    31081 agcgaaaatg gttatcgaac ctacacgcag cagcatctca acgaactgac cttactgcgc
    31141 caggcgcgcc aggtgggctt taacctggaa gagagcggcg aactggtgaa tctgtttaac
    31201 gacccacagc gtcacagcgc cgatgtaaaa cggcgcacgc tggagaaggt agcggagatc
    31261 gaacggcaca ttgaggagct gcaatccatg cgcgaccagc tactggcgct ggcgaatgcc
    31321 tgccctggtg atgacagcgc cgactgcccg attatcgaaa atctctccgg ctgctgtcat
    31381 catcgggcgg ggtgaaaatc agttaatccc cgtttgcacc gaaagaatat gctgcaaact
    31441 ttctaccatt atgcgccccg attgaaataa gccttcctgg atttcgttgt taacattcac
    31501 agcgtgaacc agggaggcta ataacaccac ttgcaagaag gcaaaaataa acgcaccgat
    31561 aatcataata tgcgtcaggg ggattttcct ttgctccttc attttaaata acccacaaac
    31621 tgaccttcct tcattaaggc gacttttccc gtgtggtgat aaacaatgac gttataaaga
    31681 cctgactgat aaacagtggc atctttattg tcttcatcaa ttgcacataa tgaatcattg
    31741 agataagcaa agttcttact gctcattcta aaggtcatgc caaatccttt ataaatagat
    31801 tttttagcgg cctgggcatg ttgtgcggct tctgcatttt taaatgcctg cgcctcggat
    31861 gcacttaaaa tagccgtctg acgctgttgt tctgcgagat aacaggtgtc atggctgacg
    31921 ccctgcgcct ggcaatctgc gatgcgggaa gtgggtgaac tacagccagc cagtaatata
    31981 accgccagca gggaaaacga tttaatggaa ctattcatca ttgttaacct taaataatgt
    32041 acggaaaatt gcgaataaga aattgctgtt aaaattagaa cgaatatccc acgccgacac
    32101 ccgcaccgaa atcagattgt gtatccgcag aaatacctgc tttaagtacc gtatgcgttc
    32161 cggcattaaa agatgttcct acagacaccg cagattcacc gttgaaggtt cccgctcccg
    32221 ctgacaccat catcaagtca ccggttttca cctgtggttg tgaggcgata gcgatggctg
    32281 atgcagttcc cgcattggct tctttacgat tgtcctcaac ttcgtttttc agagaatcga
    32341 aatggttatt gagcgtgtta atttgttggc tgttatctgc gacccgtttt tgcgtgttgg
    32401 ttaactgttg ctgcgtttgt gcggaatctt gtctcagact ttcaatttgc tcatgtgcct
    32461 ggacagactt ctgcgccaga ctttgcattt gtttatcgta ttgagccctt tctttttgct
    32521 gttgtgctgc cacatcggtt gtcagtgcat tcattttctg cccttgttcc gtggtagtgc
    32581 gctgattagc ctcattttga gccgccagcc tggcattaat catctcctga taatgcgcgg
    32641 tattttgcgt atctccagtc gcggcgagcg ttttttgtgt ggctacgatc tgcgcctgtt
    32701 gctctgtaat ttgcgtttgc tgggcatcag ttaccgcaga ctgtcgggcg ttgatcatgc
    32761 tctgataacg ggcggttgtc tgtacatcac ctgtagcggc aacggttttt tgtcctgcgt
    32821 caggatctgt ctggacaggg ggagtctgag tggtatcagg tgtttcagaa gggatgtagt
    32881 gatgttcagt caaataggta tacgcagcgg ggctgttgtc tgtgcgttct aaaaagcggg
    32941 taatcttctg ctcaatctcc gattgcgaca tgccagagtt aatggctgaa agctctgttg
    33001 ccgccgcttc aatggtatca ccagctaaaa caacagggct ggatgttgct gaaattatga
    33061 gtgccagtaa agctacgttt acagttttca ttcattttct cttgttcaaa ataagtcgca
    33121 ttaatgtttc tataagaaac aaataatatt gttttaagaa ataaactgcg ttgcaattta
    33181 atcgaaatct acgccgccat ttgtgtgatg taaagcgcat ttttgtctgg tgtaagcgta
    33241 aacaccacca ataagaaaca aaaaatgttt cttatataaa taaaatcgaa aatcattttg
    33301 tgctctttca atgtaaaaaa gagtaaagac ttgttttgat aagcgaaata aattctgttt
    33361 ctggactaaa cgtgaagcgg ttcctgatgt gtgctaaaat gcgtttcttt gataaatctc
    33421 aatttttaag aggtttacgc caggcaggaa aatttaaatg aatgaatttg agaagatttt
    33481 taatgaaatg aatctggacc gggcattgct accgatcctt tttcgttcga accggtcaac
    33541 agtgtggaaa tatttaagtg gcgatagtac ggctcctgcc tcagctatgt cactgattat
    33601 gctgttgcag ttaatccaga aacgaaaccc cgatctgtta gccgaatggt taacgctttc
    33661 tgatttcacc attccgccag aagtttacct ggaccagcct gattactgga aagggtgggt
    33721 gtatacgcaa cacaaggtca ataaaaacgt cctggaatat ctaaaaaaac attatccgga
    33781 tgaagaccaa aaaaacatga ataaaggtag tgacgagtaa tttgccaatg ccagttaata
    33841 tagcggaggc ggcctacgat tcgggcgcag acttccaggc atgataagac gcgcccgcgc
    33901 cgcatcaggc atcagcgcat aaccgccgga tgcggcgtaa acgccttatc cggcctacaa
    33961 ttcgggcata ttttgtaggc atgaaaagac gcgtcagcgt cgcatcaggc gatgaccgca
    34021 cggatgatca gcgttatccc ttctatcgca atgacttcaa catgcgtacc tgcaccgaga
    34081 tcctcgctgg cgctgacagg ccatgaactg tcaccgacgc gcatatgacc gcgcccgttg
    34141 accagtggag attccagcac aaaacgtcgg ccaatcagct gctgcccgcg ctggtttaaa
    34201 tgactgtcgc tgtgcttttg ttcgcgcacc cgccgcgaca accatttcca ccacagccag
    34261 gcggcgagca gcgtcaggac ggcaaacatc accccttgcc actcccaacc cagcggcaac
    34321 agccagacca ccaggccggt aatcaccgct gccacgccgc tccacaacag ataaccattg
    34381 ccgcccaaca tctcggctgc cagcagcaac ccgccgagac tgagccagaa aatatgtgga
    34441 tgaacgacca ttaactccat catggctgag tccgcttgtt ggcgctgtct ttcaccagct
    34501 cggcaatccc ggcaatcgac cccatcaggc tgctggcctc taatggcatc atcactactt
    34561 tgctgttact ggacgaaccg atctgctgta acgcttcggt gtatttctgc gctacgaagt
    34621 agttcaccgc ctgaatatcg ccggaggcaa tggcttcaga caccattttg gtggcgcggg
    34681 cttctgcttc agcggaacgt tcacgcgctt cggcctgtaa gaatgccgac tgacgttcac
    34741 cttccgcttt caggatttgc gactgttttt caccttcggc tttgaggatt tccgcctgac
    34801 ggatcccttc cgcttcgagg atgtaagcgc gtttggtacg ttccgctttc atctgcgcgt
    34861 tcattgaaga gataagctct gccggtgggc gcacgtcgcg aatttcaata cgggtgactt
    34921 taatccccca cgggttggtg gcctcatcga caatacgcag caggcgtgag ttgatgctgt
    34981 cgcgctgaga gagcatttca tcaagttcca ttgagcccaa cacggtacgg atgttagtca
    35041 tggtcaggtt gatgatcgcc agctccagat tgctgacttc ataggcagcg cgtggcgcgt
    35101 caatcacctg aataaagcac acggcgtcga tagtaacgtt ggcgttatct ttcgagataa
    35161 cttcctggga agggatatcg agcacttgct ccatcatatt gatcttgcga ccaatgcgat
    35221 ccataaacgg caccaccaga ctgagccccg gctgtaacgt tttggtatag cgaccaaaac
    35281 gttctaccgt ccattgatag ccctgcggca cgattttgac gcccgcgccg acaatgacta
    35341 gcgcgacaaa aatgagaata gggataaaga taagcatcgg aaaaacctcc tgttgtaccg
    35401 tccataatca gcaaaattgc tgcttgatta aacaaattat acctgattac tgaaagagag
    35461 ttccccctta ttcctgcgaa ggataaactg tttttagtaa aaatcagaaa aagggaacag
    35521 tgatgcagga aaatagtcct ttgcttcagc tacaaaacgt aggatatctg gcgggtgatg
    35581 cgaagattct taataacatc aatttttcgc tgcgtgctgg cgaatttaag ttaattaccg
    35641 gtccttctgg ttgtggcaaa agtacgctgc taaaaatagt tgcttcattg atcagcccaa
    35701 ccagcggaac gttactgttt gaaggtgagg atgtcagcac actaaagcca gaaatctacc
    35761 gccagcaagt gtcttactgc gcccagacgc cgacgctgtt tggcgatacg gtatacgata
    35821 atctgatctt tccctggcag atccgtaacc agcagcctga cccagccatt tttctcgatt
    35881 ttctcgaacg cttcgcctta ctggacagca ttttgacgaa gaatatcgcc gagctatctg
    35941 gtggtgaaaa acaacgcatc tcattgattc gtaacctgca atttatgccg aaggttttat
    36001 tgctggatga aatcaccagt gcgctggatg aaagtaataa gcataacgtt aatgagatga
    36061 tccaccgtta tgtgcgcgag caaaatattg ccgtgctgtg ggtgacacac gataaagacg
    36121 aaattaatca tgcggataag gtgattacac tgcaaccgca tgccggagaa atgcaggaag
    36181 cacgctatga actcgcataa tattactaac gaatcattgg cactggcatt aatgctggtg
    36241 gtggtggcaa tcttaattag ccataaagaa aaactggcgc tggagaaaga tattctctgg
    36301 agcgtcgggc gcgcgataat tcagctgatt attgtcggct atgtgctgaa gtatattttc
    36361 agcgtggatg atgccagcct gacattattg atggtgttat ttatctgctt taatgcggcg
    36421 tggaacgcgc aaaaacgcag taaatatatt gctaaagctt ttatctcatc gtttattgcc
    36481 attacggtcg gagcgggaat taccctggcg gtactgatcc tctctgggtc gattgaattt
    36541 atcccgatgc aggtgatccc tatcgccggg atgatagccg gtaacgccat ggtagcggtg
    36601 gggttgtgtt acaacaattt agggcaacgg gttattagcg aacagcaaca gattcaggag
    36661 aaactgagtc ttggcgcgac gccgaaacag gcttcagcga tattgattcg cgacagtatt
    36721 cgcgcggctt taattccgac ggtcgattca gcaaaaacgg ttggcttagt gagtttacca
    36781 ggaatgatgt ccgggctgat atttgccggg attgatccgg tgaaggcgat taaatatcag
    36841 attatggtga cctttatgct gctctcaacc gccagcctgt cgaccattat tgcctgctat
    36901 ttaacctatc gtaagtttta caattcgcgc caccagttgg tggtgacgca attgaagaag
    36961 aaatgatgca atgccggatg cgcagcatcg catccggcgt tgtggttcat gtgccgatca
    37021 acggaaactc aatacaacaa cgcatataac tggcggcgat acttcgacgc cagtgcatcg
    37081 ccggtaccca gcgcagcgag gatctcctgg aacgttttac gcgtctgacc gtctgcggcg
    37141 gtgagatctt tacgcagatg cccgaacagc aactccagcg cctcttcatt gcgcccaacc
    37201 tgatgcagtt gcagcgccag ttgcgtcgcc agtgcggcat cttctggatt ttccgccacc
    37261 tgctgttgca actgttgaat ttccggcgta tcagccgcct gcttcagcag ttcgatttgc
    37321 gccaccagcc cctggtagcg ggtgtcctga tcctgtaacg gaatggtttt cagcacggct
    37381 tcagcatctt cagaacggtt cagcgcaatc agcgtttctg ccagcagcag gccgatctcc
    37441 ccgttctgat tcgacaactg ccaggcgtct ttcagcaatg gcagggcgtc ggtgtaattg
    37501 ccttcttgca tcagttgcat cgcctgctgt gctttcagtt cttcttcgcg cggcagcact
    37561 ttatccagca gggcgcggat cgcctcttcc ggttgcggcc cctggaagcc gtctaccggt
    37621 tgcccgttct ggaacagata cacggtcgga atcgcgcgca gaccaaactg ggcggcaatc
    37681 atctgctccg cgtcgcagtc cagcttcgcc agaataaatt gcccgttgta ctgcgccgcg
    37741 aggctttcca gaattggggt taactgcaaa cagtgctggc tacgttcaga ccaaaaatag
    37801 aacagcaccg gagtggtcat cgactgttca agaacctgtt gcaggttaga ttcgttaatg
    37861 ttgacaatat tttctacgga catggagtcg ctctctgttg tcgatatttt ctttgacatg
    37921 ggggcttaag cgcgcgcttc aactcacccc tgcaatattt tgtccatcac gcgccccggc
    37981 agcaggcgct taagcaccat tactgcccag gtcaccagcg ttaccggata gcgcatcttc
    38041 ggtttctcgc taataaaagc atggcgcact ttgtcgacca ccgcttccgg ccccaacgta
    38101 aagcgggcgg cgatgccggg attttcgaca ggtttatcac tttgcgtctg gttgacgttg
    38161 tcggtgaagc gagtacgaat gggaccgggt tcgatcaggc tgactttaat tccgctgtgg
    38221 cgtagctcca tgcgcagcgc atccgaccac gcctccagcg catatttact ggctgcgtaa
    38281 gcgccgcgac ccggcgtgga gattaatccc atcaccgatg aggtcatcac aatacgccct
    38341 tcaccgtgcg gtaacatcgc gggtaacagg cgcatggtga gctggtgtgc gccgaaaaag
    38401 ttggcagaaa actgctgttc catctgcgca cggctgatgg tggaaagggg gccatacatg
    38461 ccgaatccgg cattgttaaa gatcccatac agacaattat cggtcagggc gatcacttcg
    38521 tcggctgcgc gatcaacact ttctggtgaa tccagatcga tcaacacgcc ggtaaatccc
    38581 atgctgttca tgcgctcaac atcatccggt ttccggcaac ctgccagcac atgaaaaccc
    38641 tggcgtttta attcgagcgc gctttccagg ccaattccac tggaacatcc ggtaattaag
    38701 accgattttt gcataacttt acctgtcagg atctccgttg ctttatgagt catgatttac
    38761 taaaggctgc aactgcttcg ccatccagtc ggcaataaac ggctgggcgt cgcggttggg
    38821 atgaataccg tcatcctgca tccattgtgg cttgaggtag acctcttcca taaaaaaggg
    38881 cagcagcgga acatcaaact ctttggcgag tttggggtaa atggcgctaa aggcttcatt
    38941 ataacggcga ccatagtttg caggcagacg tatttgcatt aacaatggtt cagcgttggc
    39001 ggctttgaca tcctgcaaaa tctggcgcag cgtttgctcg gtttgttgtg gctgaaaacc
    39061 acgcaaaccg tcattgccgc ccagttctac cagcacccag cgcggctgat gctgtttcag
    39121 cagagccgga aggcgcgcca gcccttgttg cgaggtgtcg ccgctgatgc tggcattgac
    39181 taccgacgtt ttactctgcc acttatcatt caacaaggca ggccaggccg cgctggcaga
    39241 cattcgatac ccggcgctca ggctatcacc cagaatcaat aacgtgtccg ctgcggcggc
    39301 acggaaggtt aacaggacca ggaacaggaa gggcaaatgc cagcggaaaa cattgttgaa
    39361 gttcatcatc ttaagaagtc cgtcggtcag ggggagcatg aactctccat cctcaccgga
    39421 gttgagctgg ttgtcaaacg tggcgagacc atcgcactgg tgggcgagtc gggatcgggt
    39481 aagtcaacct tgctggcgat cctcgccggg ctggatgacg gcagcagtgg cgaagtgagt
    39541 ctggtgggac aacctctaca taatatggac gaagaagcgc gggcaaagtt gcgcgcgaag
    39601 cacgtcggct ttgtttttca gtcatttatg ttaattccta cccttaacgc gctggaaaac
    39661 gtcgagcttc cggcgctgct gcgtggtgag agtagcgcag aaagtcgtaa cggggcaaaa
    39721 gcgttgctcg aacagttagg gctgggtaaa cgtctggatc atcttccggc acagctttcc
    39781 ggcggtgaac agcaacgagt ggcgctggca cgagccttta acggtcgacc tgatgtgctg
    39841 tttgccgacg aacccaccgg caaccttgac cgccagacgg gcgataaaat tgccgacctg
    39901 ctgttttccc tcaaccgtga acatggcacc acgttgatta tggtgaccca cgacctgcaa
    39961 ctggcggcac gctgcgaccg ctgcttacgg ctggtgaacg ggcagttgca ggaggaagca
    40021 tgattgcacg ttggttctgg cgcgaatggc gctcgccgtc gctattaatt gtctggctgg
    40081 cgctaagcct ggcggtggcc tgcgtgctgg cgctgggcaa tatcagcgat cgcatggaga
    40141 agggcttaag ccagcaaagc cgtgagttta tggcgggcga tcgggcgttg cgcagttcgc
    40201 gcgaagtgcc gcaagcgtgg ctggaggaag cgcaaaagcg cggcctgaaa gtcggcaagc
    40261 agctgacttt cgccaccatg acatttgcag gcgacacacc gcagctggcg aacgtcaagg
    40321 cggtggatga tatctacccg atgtatggcg atctgcaaac caatccccct ggcctgaaac
    40381 cgcaggcggg cagcgtattg ctggccccac gcctgatggc actgcttaac ctaaaaacgg
    40441 gcgacaccat tgacgtgggc gatgccacct tgcggattgc cggagaagtg attcaggaac
    40501 cggattccgg ttttaacccc ttccagatgg ctccgcgtct gatgatgaat ctggcggatg
    40561 tcgataaaac cggagccgtg cagccgggga gtcgggtcac ctggcgctat aaattcggcg
    40621 gcaacgagaa ccagctcgac agctatgaga aatggttgtt acctcagctt aaacccgaac
    40681 aacgctggta cggtctggaa caggacgaag gcgcgctggg gcgctcgatg gaacgctcgc
    40741 aacagttcct gctgctttcg gcgcttctga ccttgctgct ggcagtggca gcggtggcgg
    40801 tggcgatgaa tcattactgt cgcagtcgct acgatcttgt ggcgatcctc aaaacgctgg
    40861 gggcagggcg agcgcaactg cgtaagctaa tcgtcggtca gtggttgatg gtgctgacgc
    40921 tttcagccgt taccggtggg gcgataggcc tgttgttcga aaacgtgttg atggtgctgc
    40981 tcaagcccgt tctgcctgct gcactaccgc cagccagcct ctggccgtgg ctatgggcgc
    41041 tgggcaccat gacggttatc tcgctgctgg tggggctacg accataccgc ttgttactgg
    41101 caacgcagcc tttacgcgta ttacgtaatg atgtggtcgc gaacgtctgg ccgctgaagt
    41161 tttatctgcc gattgtcagt gtggtggttg tgctgctgct cgccggatta atgggtggca
    41221 gtatgctgct ctgggcggtg ctggcgggcg cggtagtact ggctttgctg tgcggtgtgc
    41281 tgggctggat gctgctgaat gtacttcgcc gcatgacgct gaaatcgctg cctctgcgcc
    41341 tggcggttag ccgcctgtta cgtcagccgt ggtcaacgtt aagccagctt tcggcatttt
    41401 cgctctcctt tatgctgctg gcgctattgc tggtattgcg tggcgatctg ctcgaccgct
    41461 ggcaacagca gctaccgcca gaaagcccga actacttttt aatcaacatc gccacagaac
    41521 aggtagaacc gctaaaagcg ttcctcgcgg aacatcagat agtcccggaa tcgttttatc
    41581 cggtggtgcg ggcgcggctg acggcgatta acgataagcc gacagaaggt aatgaagatg
    41641 aggcgcttaa ccgcgaactc aatcttacct ggcaaaatac gcggcccgat cataatccga
    41701 ttgtcgccgg taactggccg ccaaaagccg atgaagtgtc gatggaagag gggctggcga
    41761 aacgcttaaa cgttgccctc ggcgatactg tgacttttat gggcgatacc caggagttcc
    41821 gcgctaaagt gaccagcctg cgcaaagtgg actgggaaag tctgcggcct aatttctatt
    41881 ttattttccc tgaaggggca ttagacgggc aaccgcagag ctggcttacc agtttccgct
    41941 gggagaatgg caacggcatg ttgacgcaac tcaaccgcca gttcccgacc attagcctgt
    42001 tagacattgg cgcgatttta aaacaggtcg ttcaggtgct ggagcaggta agtcgggcgc
    42061 tggaggtgat ggtggtgctg gtcaccgcct gcggtatgtt gctgttgctg gcacaggtgc
    42121 aggtgggaat gcgtcagcgt catcaggagc tggtggtgtg gcgcacactc ggtgcgggga
    42181 aaaaactgct gcgtaccacg ttgtggtgtg agttcgccat gctcgggttt gtttctggcc
    42241 tggtggccgc aattggtgcg gaaacggcgc tggcagtgtt gcaggcgaaa gtgtttgatt
    42301 tcccgtggga gccagactgg cgattgtgga ttgtgctgcc gtgcagcggt gcactgctgc
    42361 tgtcgctttt cggcggctgg ctgggcgcgc ggctggttaa gggtaaggcg ctgttcaggc
    42421 agtttgcggg gtgatgagag taaataaggg gcgttctggt tgaatggaac gccttgttta
    42481 aattaattct acaatatgtt tcattgatga tgttattgaa ttggtgattt cctatctttc
    42541 tattgctgat attaatctga atcggtggag tttaataatt ttaaatatag ccctacaaga
    42601 ataactcaga ctaataaaat cataaaccat atgcgttgaa tggatattat ccatatagtg
    42661 aatttgttga tgatgaattc atctgtgcta aaaatgttag tttaataaaa tattgaaagt
    42721 gacctgtaat aacagttgtt gttgattgag aacaaataag tttatgtgaa aaatatataa
    42781 atacattagc tggtcttgtg tgtcatttta tttttttgtt gctaacacag gaatatgaac
    42841 aataactaaa agggcacttt a
//
LOCUS       DAPOZO010000038        42618 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPOZO010000038 DAPOZO010000000
VERSION     DAPOZO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.038"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(182..1039)
                     /gene="murI"
                     /locus_tag="ROI21_002151"
     CDS             complement(182..1039)
                     /gene="murI"
                     /locus_tag="ROI21_002151"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDX5111070.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIITAVQKRYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
     gene            complement(984..2828)
                     /gene="btuB"
                     /locus_tag="ROI21_002152"
     CDS             complement(984..2828)
                     /gene="btuB"
                     /locus_tag="ROI21_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDX5111071.1"
                     /translation="MIKKASLLTAFSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYFSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVTVGHGNIGAG
                     VDWQKQSTAPGTAYVKDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2893..3083)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3197..4297
                     /gene="trmA"
                     /locus_tag="ROI21_002153"
     CDS             3197..4297
                     /gene="trmA"
                     /locus_tag="ROI21_002153"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDX5111072.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDGETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4337..4696)
                     /locus_tag="ROI21_002154"
     CDS             complement(4337..4696)
                     /locus_tag="ROI21_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDX5111073.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4696..5346)
                     /gene="fabR"
                     /locus_tag="ROI21_002155"
     CDS             complement(4696..5346)
                     /gene="fabR"
                     /locus_tag="ROI21_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDX5111074.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5678..7078
                     /gene="sthA"
                     /locus_tag="ROI21_002156"
     CDS             5678..7078
                     /gene="sthA"
                     /locus_tag="ROI21_002156"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDX5111075.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7061..7978)
                     /gene="oxyR"
                     /locus_tag="ROI21_002157"
     CDS             complement(7061..7978)
                     /gene="oxyR"
                     /locus_tag="ROI21_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDX5111076.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVVAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKKAV"
     gene            complement(8245..9618)
                     /gene="argH"
                     /locus_tag="ROI21_002158"
     CDS             complement(8245..9618)
                     /gene="argH"
                     /locus_tag="ROI21_002158"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDX5111077.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9679..10455)
                     /gene="argB"
                     /locus_tag="ROI21_002159"
     CDS             complement(9679..10455)
                     /gene="argB"
                     /locus_tag="ROI21_002159"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDX5111078.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10463..11467)
                     /gene="argC"
                     /locus_tag="ROI21_002160"
     CDS             complement(10463..11467)
                     /gene="argC"
                     /locus_tag="ROI21_002160"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDX5111079.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11621..12772
                     /gene="argE"
                     /locus_tag="ROI21_002161"
     CDS             11621..12772
                     /gene="argE"
                     /locus_tag="ROI21_002161"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDX5111080.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALREVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            13074..15725
                     /gene="ppc"
                     /locus_tag="ROI21_002162"
     CDS             13074..15725
                     /gene="ppc"
                     /locus_tag="ROI21_002162"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDX5111081.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15908..17641
                     /locus_tag="ROI21_002163"
     CDS             15908..17641
                     /locus_tag="ROI21_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDX5111082.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQAAHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17790..18641
                     /locus_tag="ROI21_002164"
     CDS             17790..18641
                     /locus_tag="ROI21_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5111083.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18628..18969)
                     /locus_tag="ROI21_002165"
     CDS             complement(18628..18969)
                     /locus_tag="ROI21_002165"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDX5111084.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFDHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18971..19849)
                     /locus_tag="ROI21_002166"
     CDS             complement(18971..19849)
                     /locus_tag="ROI21_002166"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDX5111085.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSVDVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19815..22112)
                     /locus_tag="ROI21_002167"
     CDS             complement(19815..22112)
                     /locus_tag="ROI21_002167"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDX5111086.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASRKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQCEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22163..22483)
                     /locus_tag="ROI21_002168"
     CDS             complement(22163..22483)
                     /locus_tag="ROI21_002168"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDX5111087.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTV"
     gene            complement(22498..23577)
                     /locus_tag="ROI21_002169"
     CDS             complement(22498..23577)
                     /locus_tag="ROI21_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDX5111088.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLVAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23886..26387
                     /gene="ptsP"
                     /locus_tag="ROI21_002170"
     CDS             23886..26387
                     /gene="ptsP"
                     /locus_tag="ROI21_002170"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDX5111089.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAYQRLSQWLRDEFPHCDAPLVEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVMKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     AHHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26399..27061
                     /gene="fsa"
                     /locus_tag="ROI21_002171"
     CDS             26399..27061
                     /gene="fsa"
                     /locus_tag="ROI21_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDX5111090.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27072..28175
                     /gene="gldA"
                     /locus_tag="ROI21_002172"
     CDS             27072..28175
                     /gene="gldA"
                     /locus_tag="ROI21_002172"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDX5111091.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGISETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            28506..29123
                     /locus_tag="ROI21_002173"
     CDS             28506..29123
                     /locus_tag="ROI21_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDX5111092.1"
                     /translation="MKASLALVSLLTAFTSNSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFSRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSHGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29150..30055)
                     /gene="yijE"
                     /locus_tag="ROI21_002174"
     CDS             complement(29150..30055)
                     /gene="yijE"
                     /locus_tag="ROI21_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDX5111093.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSALLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSAVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKNI"
     gene            complement(30149..32329)
                     /gene="katG"
                     /locus_tag="ROI21_002175"
     CDS             complement(30149..32329)
                     /gene="katG"
                     /locus_tag="ROI21_002175"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDX5111094.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQEPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDCVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32658..33548)
                     /gene="metF"
                     /locus_tag="ROI21_002176"
     CDS             complement(32658..33548)
                     /gene="metF"
                     /locus_tag="ROI21_002176"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDX5111095.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSNLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33930..35483)
                     /locus_tag="ROI21_002177"
     CDS             complement(33930..35483)
                     /locus_tag="ROI21_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDX5111096.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHDFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAKSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMDLTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATIYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQI
                     NGMRVKDIK"
     gene            35616..36746
                     /locus_tag="ROI21_002178"
     CDS             35616..36746
                     /locus_tag="ROI21_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDX5111097.1"
                     /translation="MSTLFTINACKSFGCRNLGQPTSTDYSWPDYRLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTFATEKGHFCPVCYGTKTICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITPPEIIETVALLVPFQGASGGQKLYVLISFDALRGNILHLSTNYTQH
                     QAGESLHYRYRGNAEPELHESNIVQRVDMREAQFLRRSQFDEIQYGSAALKRNAKGVI
                     LRPVITAHGHFRVLNILFPTVKTHVISHECFLRGAIITAWADLFRQQQGEIWFIEEEI
                     ADDTDNMPWRFQGTTYHGWWKNQWQLWVQGKNRKMVCALTGGNNSKAEMLSLATSRHF
                     IDWLHKQAVFTHSAPLSAGRVTQILLSLAQDYNNCARRLISD"
     gene            complement(36710..39142)
                     /gene="metL"
                     /locus_tag="ROI21_002179"
     CDS             complement(36710..39142)
                     /gene="metL"
                     /locus_tag="ROI21_002179"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDX5111098.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     ISDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEEGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYDIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(39145..40305)
                     /gene="metB"
                     /locus_tag="ROI21_002180"
     CDS             complement(39145..40305)
                     /gene="metB"
                     /locus_tag="ROI21_002180"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDX5111099.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            40582..40899
                     /gene="metJ"
                     /locus_tag="ROI21_002181"
     CDS             40582..40899
                     /gene="metJ"
                     /locus_tag="ROI21_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDX5111100.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            40959..41567
                     /locus_tag="ROI21_002182"
     CDS             40959..41567
                     /locus_tag="ROI21_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDX5111101.1"
                     /translation="MIIRLLIPGLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVKQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSSPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            41712..42387
                     /locus_tag="ROI21_002183"
                     /pseudo
     CDS             41712..42387
                     /locus_tag="ROI21_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT        10421 a        11669 c        10641 g         9887 t
ORIGIN      
        1 ttcccgctac agagtcaagc atttttttgc ttttctctgt tgagattctc aggagaaccc
       61 cgctgacccg gcgacgtgtt tgccgttgtt ccgtgtcagt ggtggcgcat tatagggagt
      121 tattccggcc tgacaagagg aaatttaaaa taattttctg accgcgcaac attcaaccaa
      181 atcagtctaa aactgccagt ttttcgagcg tttcgaagcc gtaacgctgt aaaacgggca
      241 ataattgttc agcttctggc gtcattgcca tacaaaaggc aatattcgca tcggcagatt
      301 ttgcatccgg ggcttcatgc tctagcaacc aggccgttcg gcgagcaatc gctgcgccag
      361 aatccaccag ccgggttccc tctggcagca cctgtaacag ttcttcttgt agtagaggga
      421 aatgggtaca ccccagtaca acggtatctg gcggctcttt cattcttaac cacggacgca
      481 agatacgttt tagcgcatcc agagaaacat cttcgccatg cagcttcgct tcagccaact
      541 caaccatctc tgccgagccc agcatttcta tctggcattc attagcgaaa cgcgcgatca
      601 gctcatgagt ataagaacgt ttaactgttc cgcgggttgc cagtaatccg acaatgccat
      661 ttgccgtcag acgtgcagca ggtttaatcg ccggcacgac accaacaacc gggaagtcga
      721 acttttcgcg taatgcagga agtgaaacgg tactggcagt gttgcaagcg accacagcca
      781 gcgcaagggg ataacgtttt tgcactgcgg tgataattgc caccactcgc tcaacaataa
      841 acgcttcgct tttttcgcca tacgggaaag cgacgttatc gaaagcataa atgtaatgga
      901 gatccggtaa gagatgccgg atctcgtcat agaccgacaa cccaccgacg ccggagtcaa
      961 acaccagcac ggtgggacgt ggttcagaag gtgtagctgc cagacaaggt gtattcccgt
     1021 cctgcagttt ggtagccata gactgtctca taatctttgt cgaacaggtt ggctatttta
     1081 ccacgaactg tcaggtgaga ggtgaccgga tacgcaaccg caagatccca caagctcaca
     1141 ccacccattt taacggtttg ataaggataa gatgagtaat ccttatcata gcgagtgcct
     1201 aaatactgat aagtaatacc ccagtcgaag tcatacaact gccagtcgag ctggtatttc
     1261 acctgctgtt tagcacggcg taacaacggc gtgtcagtaa tcgcattgcg cgcatcgaca
     1321 taatcataac tcacagtatg cgtcagtggt ccggtatcaa aattggcggt cgcctcgacg
     1381 cccttaatcc gcgctttccc ttcgttgtaa tatttcaggg tgtgatcatc ataatcgatc
     1441 aaatcactga catcgttacg atatccggaa atacgccagt tcaccccagc ggttaagcct
     1501 tcaaacgcgc cttcccactg tttgcttttc tcaggattca gattcggatt tccgtagaaa
     1561 ccatacagtt gcccaagatt aggtgccttg tacgacgtgc cataagaagc gataaaacga
     1621 tagccttcga tgaattccca accggcactc gtttgccagg ttccatgacg accaaactgt
     1681 gagttatcgt cgctgcgggc tgcgccttca aacgtaaaat cgccgacttg ttgcagccca
     1741 gtcagataga tgccggtatt acgttgatca tatccatcct taacataagc tgtgcccggt
     1801 gccgtgctct gcttctgcca gtcaacgccc gcaccaatat taccgtggcc aacggtaacg
     1861 ttatttgccc actggacggt gtactgcttc atctcatcca gcgtcgcgga cgaatcataa
     1921 cggccataat ggggatcgta gttgtaatct ttgctatggc tatagctggt aatgagttgt
     1981 gatttaatca gttcaccgtt atagcgcagc ccggcgtccc aactttggct atagagttta
     2041 cgggtatcga gcaacggtga gccgggagaa aaatacgcgt cataattggt acggttatca
     2101 tagccatagc cgcgcacaaa gccgctccag gcatcagtaa agttatgctc cagcgcgcca
     2161 taaagcgttt tacttaaaaa accatcgtta tctgtctgcg cttgcgttcc ggtattccca
     2221 taggcaacaa catcataacc atgagtatgg gcataatcgc ccaacagcgt tacccgtgtc
     2281 ttatccccca gttgttgctg cgtggagaca tcatagttct gataactatt gcttccccac
     2341 cctgctgaaa tttccgttcc gggttcatcg cgcgtcgtga tgatattcac caccccgcct
     2401 attgcatcgg aaccataaac agcggagcgc ggtccacgga tatattcaat acgctggaca
     2461 agcgcaatag ggaactggct aaggtcggca gaaccactca ctcccgccag attcaggcgg
     2521 acgccatcaa ttaacaccag cacatgactg gcatttgtac cgcgaataaa aatagatgag
     2581 agctgacctg aaccgccgtt ttgggtgata tcgacacccg gaagacggcg cagaacatca
     2641 ttgactgagg tcgactgcca gcggtcaata tcctgacgcg tcacaacggt ggttggtgca
     2701 agcacagtgc tgcgcggctg ttcaaaacgg ttagcagtaa caacgagagt atccgggctg
     2761 gtatcctgtg cccaagcgga aaaggctgtg acggaaaacg ccgtcagcag cgaagctttt
     2821 ttaatcattg taaagcatcc acaatagaag aaggatgccg caggtttcat caatattacg
     2881 cgatgatgag aaccagatgc gacgttagcc ggcaggtctt cgggcttgga ggggtatcta
     2941 agatactaag agatgatgac ttcccaccgg atggcagtgt ccgcataacg caatcatcgc
     3001 acctttcctt accgctgcgc gtcagctcca gatttgcact ggattcccta ttaactcaca
     3061 ggaccggcga gtggatgcta caggttgtaa caagttactg tccagacgta gctcacaaat
     3121 aggaatacat caagatctgg acatctgatg agcaatccct acaatcgccg cgtactttaa
     3181 tttttcagga tacatcatga cccccgaaca ccttccaaca gaacagtatg aagcgcagtt
     3241 agccgaaaaa gtggtacgtt tgcaaagtat gatggcaccg ttttctgacc tggttccgga
     3301 agtgtttcgc tcgccggtca gtcattaccg gatgcgtgcg gagttccgca tctggcacga
     3361 tggcgatgac ctgtatcaca tcattttcga tcaacaaacc aaaagccgca tccgcgtgga
     3421 tagcttcccc gccgccagtg aacttatcaa ccagttgatg acggcgatga ttgcgggtgt
     3481 gcgtaataat cccgttctgc gccacaagtt gttccagatt gattacctca ccacgctgag
     3541 taatcaggcg gtggtttccc tgctgtacca taagaagctg gatgatgagt ggcgtcagga
     3601 agcggaagcc ctgcgcgatg cactgcgagc gcagaatctg aatgtgcatc tgattggtcg
     3661 ggcaacgaaa accaaaatcg agctggatca agattacatc gatgaacgtc tgccggtcgc
     3721 agggaaagag atgatctacc gtcaggtaga aaacagcttt acccagccga acgcggcgat
     3781 gaatattcag atgctggaat gggcgctgga cgtaaccaaa ggctcaaaag gcgatttact
     3841 ggagctgtac tgcggcaacg gtaacttttc attagcactg gcgcgcaatt ttgatcgggt
     3901 attagccacc gaaatcgcta agccgtcggt cgctgccgcg caatacaaca ttgccgctaa
     3961 ccatattgat aacgtacaaa ttattcgtat ggcggcagaa gaatttactc aggcgatgaa
     4021 tggcgtacgc gagtttaacc gcctgcaagg gatcgattta aagagttatc agtgcgaaac
     4081 catttttgtc gacccaccac gcagcggtct ggacggtgaa accgagaaaa tggtgcaggc
     4141 gtatccacgt attctgtata tctcctgcaa tccggaaacg ttatgcaaga atctggaaac
     4201 attaagccag acgcacaagg ttgaacgtct ggctctgttt gatcagttcc cctacacgca
     4261 ccatatggag tgcggcgtat tactgaccgc gaagtaaaac ccatgccgga tgcatcagca
     4321 tccggcatga ttccgattac tcagcaactt cctgcttacg gttacgcatc ttcgcgccaa
     4381 tccagaacac cataatgacg gagagtactg ccgggaagaa gttagagccg atatccggat
     4441 actccgcacg aactaccgtg ctgtacagca gtacgccgag aataaaacag gcagcagcca
     4501 gacccggcaa gcctaccggc atggtgcgat taagataacg ttgatgcagg caataaaccg
     4561 tcagcaccag ggaaataatc gggaatacag aaaatggcac aatggagcta aacagcgctg
     4621 cgaaagtacc attaatcgat aagccagcca ccagcgccag cagcagcgta cctctatctt
     4681 gatttgcttg tttcattact cgtccttcac atttcccgga ataattgcgg ttttctcttg
     4741 ttcacggcga taccagtaat aagccccttt cgaaatcatt cgcagttgca gtaccagtcg
     4801 ctcttctaat tgccgacgtt gttcgacgcc gacgtccaac gcctcggccc ccgcactgaa
     4861 gacaatcgtc accatggctt cggcttgcgc ttcagtaaac gcacgcggca tatggttttc
     4921 gagttccaga tagtccgcaa gttccgcaat gaagtgctga atttcacgcg caacggcggc
     4981 acgaaacgca gcggaggtgc cggagcgttc ccgcaataat aaccggaagg cgttaggatt
     5041 attaccgatg aactccataa atgtggagac cgaggtacgg atcacactcc cgcctttggc
     5101 gatacgctga cgcgcctggc gcatgagttg gcgtagcatt aaaccgctct catcaaccat
     5161 ggtcagaccc agttcgtcta cgtcgcggaa atgccgataa aaagaggtgg gagcaatgcc
     5221 cgcttcacgc gccacttcac gcaaactcag gctggcgaag ctgcgttcag cacttaattg
     5281 gctaaatgcg gcttccacca gcgaacggcg ggttttttct ttttgttgcg ctcttacgcc
     5341 catcacgatg tctgaatcct tgccaaaagt actgctggca ctataccaga gaatgaacat
     5401 actggattgc tccagtgttg tgaatgaacg gtaacgcaat aaaacatatt tactgcaatt
     5461 ctttggccgg ttcttttacg tacaacggaa acctgccgct taaacggaga gtatcgtcga
     5521 taaaaatcca ataaaacgtt caggtcaaaa gtaagaaaca gacaaagcaa aggccgctca
     5581 cgatatagcc agataaatga cggggatcaa ttggcttacc cgcgataaaa tgttaccatt
     5641 ctgttgcttt tatgtataag aacaggtaag ccctaccatg ccacattcct acgattacga
     5701 tgccatagta ataggttccg gccccggcgg cgaaggcgct gcaatgggcc tggttaagca
     5761 aggtgcgcgc gtcgcagtta tcgagcgtta tcaaaatgtt ggcggcggtt gcacccactg
     5821 gggtaccatc ccgtcgaaag ctctccgtca cgccgtcagc cgcattatag aatttaatca
     5881 aaacccacta tacagcgacc attcgcgact gctccgctct tcttttgccg atatccttaa
     5941 ccatgccgat aacgtgatta atcaacaaac gcgcatgcgt cagggatttt acgaacgtaa
     6001 tcactgtgaa atattgcagg gaaacgctcg ctttgttgac gagcatacgc tggcgctgga
     6061 ttgcccggac ggcagcgtcg aaacactaac cgctgaaaaa tttgttattg cctgcggctc
     6121 tcgtccatat catccaacag atgttgattt cacccatcca cgcatttacg acagcgactc
     6181 aattcttagc atgcaccacg aaccgcgcca tgtacttatc tatggtgctg gagtgatcgg
     6241 ctgtgaatat gcgtcgatct tccgcggtat ggatgtaaaa gtggatctga tcaacacccg
     6301 cgatcgcctg ctggcatttc tcgatcaaga gatgtcagat tctctctcct atcacttctg
     6361 gaacagtggc gtagtgattc gtcacaacga agagtacgag aagatcgaag gctgtgacga
     6421 cggtgtgatc atgcatctga agtcgggcaa aaaactgaaa gctgactgtc tgctctatgc
     6481 caacggtcgc acgggtaata ccgattcgct ggcgttacag aacattgggt tggaaactga
     6541 cagtcgtgga cagctgaagg tcaacagcat gtatcagacc gcgcagccgc acgtttacgc
     6601 ggtgggcgac gtgattggtt atccgagtct ggcgtcggca gcctatgacc aggggcgcat
     6661 tgccgcgcag gcgctggtga aaggtgaagc caccgcacat ctgattgaag atatccctac
     6721 cggcatttac accatcccgg aaatcagctc tgtgggcaaa accgaacagc agctgaccgc
     6781 gatgaaagtg ccatatgaag tgggccgcgc ccagttcaaa catctggcac gcgcacaaat
     6841 cgtcggcatg aacgtgggca cgctgaaaat tttgttccat cgggaaacga aagagattct
     6901 gggtattcac tgctttggtg agcgcgctgc cgaaattatt catatcggtc aggcgattat
     6961 ggaacagaaa ggtggcggca acactattga gtacttcgtc aacaccacct ttaactaccc
     7021 gacgatggcg gaagcctatc gggtagctgc gttaaacggt ttaaaccgcc ttttttaaca
     7081 ctttatcgaa atggccatcc attcttgcgc ggatggcctc tgccagctgc tcatagcggc
     7141 tgcgcagcgg tgagccagga cgataaacca gcccaatagt gcggcgtggt tccggcttaa
     7201 tgcacggcag ataaacaacc ccatcgcgtt tgcgctccgg cggcacagcc agtgcaggca
     7261 gtaaagtgat cccgctacct gccaccacca tgttgcgcag agtttccagg ctggtcgcgc
     7321 ggaagtgtgt atcttcatcc gccccggctt caaaacagaa acccattgcc tgatcgcgca
     7381 aacagtgacc gtcttccagc atcagcagtt tttcccctgc cagatcggcc atcggtacgc
     7441 attcgcggtt cgcccacggg tgatcttcat agatagccag cagcattggt tcatcaaaca
     7501 atggcacttc aatgaatgct tcgctctctt tcaccagcgc gaggatcacg cagtcaagtt
     7561 tgccactgtc cagttgcgcc agtaactggt gggtctgtgc ttcatgcaga tacatttcca
     7621 gctttggaaa ggtctggtgc agcatcggga taatatgcgg tagcaggtac ggtccaaccg
     7681 tgggaatcaa accaatatgc agcggtccgg acatcgtctc accctgctgg cttgccatct
     7741 ctttaaggac tttcacctca cgcagcacgg tacgcgcctg atccaccagc agcattcccg
     7801 cctgggtgaa caatacttta cggctggtcc gctccagcaa catcacgccc agctcatctt
     7861 ccagcttacg aatttgcccg ctaagcgtcg gctggctaac gtggcaggaa tctgccgcac
     7921 gccgaaaatg gcggtgttca gccaatgcca ccaggtactc aagatcacga atattcatta
     7981 tccatcctcc atcgccacga tagttcatgg cgataggtag aatagcaatg aacgattatc
     8041 cctatcaagc attctgactg ataattgctc acagaaacgg agcggcacct cttttaaccc
     8101 ttgaagtcac tgcccgtttc gagagtttct caactcgaat aactaaagcc aacgtgaact
     8161 tttgcggatc tccaggatcc gctttttttt gccataaaaa agcccggcga taagccaggc
     8221 tcaaatttgt acatataaat gttcttaccc caaccgagcc tgcgcaaaag caatcgcctg
     8281 cgccacctgc tgcggtgaga cgccgccttt tgccgcacgc ttgtcgaggc acgattgtag
     8341 cgacagaatc ggatagacat cttcgccaat cacctgactg aatttctgca actcactgag
     8401 cggtagttct tccagcggtt tgccctgacg aatggcttcc accaccgctt caccaacaat
     8461 atggtgcgcc tcgcggaacg gtacgccttt cgccaccagg taatccgcca gttcagtggc
     8521 gttggcgtaa ccctgctgag ccgcttcctg gcaacgcgga cgtttcacct gaatgccgtc
     8581 cagcaccagc gccgccatat gcaggcagtc cagccaggta tcgagcgcgt cgaacagacc
     8641 ttctttgtct tcctgcatat ctttgttgta agccagcggc aaacctttca gcgtcatcat
     8701 catgccggtt aacgcgccct gcacacgacc gcatttaccg cgaatcagct ccagcgcatc
     8761 cgggtttttc ttctgcggca ttaatgatga acctgaagtc acgcggtcag aaagctccac
     8821 aaatcccgct tcgccggtgt taaagaaaat cagatcttca gcaaaacgcg acagatgcac
     8881 catgccgata gcggcagcag aaagcagttc cagcacatga tcacggtcag aaacgctgtc
     8941 cagactgtta cgggtcgctg aggcaaatcc cagccagcct gctaactgtt cacggtcgat
     9001 ttcataggcc gttcccgcca gtgcgccaca gcctaacgga ctaacatcca gacgcttaag
     9061 cgcatcctgc aagcggcttt catcacgcgc cagcatctca acataggcca ggcaccagtg
     9121 cgcgaacgtc accggctggg cgcgttgcag gtgagtgtaa cctggcatta ccgcgtcctg
     9181 attgttctgc gcggtttcca ccagcgccga ttgcagttgg cggttagccg tcagcaactc
     9241 gctaacggta tctttgcacc acagtttcag gtcagtcgcg acctggtcat tacggctacg
     9301 cccggtgtgc agttttttgc ctaactgacc cactttgtcg atcagtttgc cttccaccca
     9361 gctatggata tcttcggcgt cgctttcaag gatttgttgt ggcctggcgc gaacatcttc
     9421 cagcagcacg ttcagcgcct cttccagttg cgcctgctct tctgcggtca gcacaccgac
     9481 cgttaccagg gctttggacc aggccacaga gccaacaata tcctgctccg ccagacggta
     9541 atcaaaacgc agtgagtcgt tgaattgttt gaaccgttga tctgctgcct gggtaaaacg
     9601 cccgccccaa agtgccataa ctctgtttcc ttattttgaa atttaatgcc ggaaagctgc
     9661 gcctccggct aacaaaactt aagctaaaat ccgcgtaccc atcggcatac cgttaaacag
     9721 tgccggaagc tgttccgcat gacgccagga ggcgatatcc accgggcggc ccagcgtgcg
     9781 cgcagcatcc agcgccgcgt tcactttcac tatcatgccg tcagtaataa tgccctgctc
     9841 aatcagttgt tctgctttcg cggcggtcat ttcggcaata cgttgccctt tgccgtcgag
     9901 aatgccgctg acgtcggaga gcaaaatcag atccgcgccc agcgttgccg ccagcgccgt
     9961 tgccgcctga tcggcattga cgttcatcag ttgcccttcg tctgttacgc caatggagct
    10021 gaccaccggc agataaccgt tctccagcaa ggtgttgata agcttaggcg aacctggctg
    10081 cgccagtcca acatggccta actcttcatc aagctgggtc actttgacgc tgtcgccgtc
    10141 accgagaaac aaacctacgg ccgcaatctg atgtttcttc gcccatgcca gcagggtttt
    10201 atttgccgtt cccgccagtg ctccggtgat aatgtcaatc tgatcagcag gcgtcacccg
    10261 caggccgttt ttctttttca ccggcagatt cagccctttc atcagctcat ccaccacgca
    10321 accgccgccg tgcacaatca ccagcggacg ctgatgtgac tcacgataat tcaccagtgc
    10381 gctaaacaga cgttccagcg cctcttcact atccagcagt acgccgccca gtttgataat
    10441 taatggattc atcattgcac ccttaaataa gagactgcgt ttccgcatag ccgaaacgaa
    10501 tattggcgca ctgtaccgct tgtgccgccg cgcctttcag taagttgtct tcggtcgcca
    10561 caatgatcaa atgctcgccc tgaacggcaa acccgatatc gcaaaatggc agcccaacga
    10621 catttttcag cgccggaacg cctttgtcat acagccgcac cagcggttta tgggcatacg
    10681 cctgctgtaa cgcttgcgcg acttgcgcct gggtcacacc cgatttcagg cggcaggtaa
    10741 tggtttcgag aatgccgcgc gggaaattgc ccagatgcgg ggtgaagata acgtcagcac
    10801 cgaggtgtgt ggcgatctct ggttgatggc gatgagtaaa gacgccatac ggttgcaggc
    10861 tcacttcaca aaaactattt gaaatcgctg ccttacgtcc tgcaccgctc acgccgctgg
    10921 tggcgttgat taccggccac tgattgaggt caagaagatc ggcatcaatc aacggtttca
    10981 gcgccagctg tgccgccgtc ggataacagc ctggcacggc aatcaagttc gcttctttta
    11041 atttattacc gcaccactcc gccagaccgt aggctgcctg ttccagcagt tccggatact
    11101 gatgggtaaa gccgtaatat ttttcataga aggtggcgtc gttaacacga aacgcgccgg
    11161 aaaggtcgaa caccacgcag ccggcttcaa gaaattgcgg cgctaaatcg tggctaactt
    11221 catgggcggt ggcgagaaac accacgtcca cccctgggct aaactcgctg atatccgaca
    11281 tcggctgcaa cggcagatca acgatgcctt ttagctgcgg atgcaaatcg gagattaact
    11341 ttcccgcatc attgctttgc gctgaaacag tcaaagcggt tatgttcata tgcggatggc
    11401 gatttacata ggtcactagc tctgcgccag cgtagccgct ggcacccaca atcagcgtat
    11461 tcaacatcgg ggctattcac cttcttatgt ctggttgcca ggttaaacgt aaaacattca
    11521 ccttacggct ggtgggtttt attacgctca acgttagtgt atttttattc ataaatactg
    11581 catgaatatt gatactatca tgaccagagg tgtgtcaaca atgaaaaaca aattaccgcc
    11641 atttatcgag atttaccgtg ctctgattgc cacaccttca ataagcgcca cggaagaggc
    11701 actcgatcaa agcaatgcag atttaatcac tctgctggcg gactggttta aagatttagg
    11761 cttcaatgtg gaagtgcagc ctgtgccagg aactcgcaac aaatttaata tgctggcaag
    11821 taccggacag ggggctggcg gtttgttgct ggcggggcat accgatacgg tgccatttga
    11881 tgacggacgc tggacgcgcg atccgtttac actgacggag catgacggca agctttacgg
    11941 cttaggcacc gccgacatga aaggcttttt tgcgtttatc cttgatgcgc tacgcgaagt
    12001 cgacgtcacg aaactgaaaa aaccgctcta cattctggcg actgccgatg aagaaaccag
    12061 tatggccgga gcgcgttatt ttgccgaaac taccgccctg cgcccggatt gcgccatcat
    12121 tggcgaaccg acgtcactac aaccggtacg cgcacataaa ggtcatatct ctaacgccat
    12181 ccgtattcag ggccagtcgg gccactccag cgatccagct cgcggagtta acgctatcga
    12241 actaatgcac gacgccatcg ggcatatttt gcaattgcgc gataacctga aagaacgtta
    12301 tcactacgaa gcgtttaccg tgccataccc tacgctcaac ctcgggcata tccacggtgg
    12361 cgacgcttct aaccgtattt gcgcttgctg tgagttgcat atggatattc gtccgctgcc
    12421 aggcatgaca ctcaatgaac ttaatggttt gctcaacgat gcattggctc cagtgagcga
    12481 acgctggccg ggtcgtctga cggtcgacga gctgcatccg ccgatccctg gctatgaatg
    12541 cccaccgaat catcaactgg ttgaagtggt tgagaaattg ctcggagcaa agaccgaagt
    12601 ggtgaattac tgtaccgaag cgccgtttat tcaaacgtta tgcccgacgc tggttctggg
    12661 gcctggctca attaatcagg ctcatcagcc tgatgaatat ctggagacga ggtttatcaa
    12721 gcccacccgc gaactgatca cccaggtgat tcaccatttt tgctggcatt aaaacgtagg
    12781 ccaaacaatg tcggatgcga tgcttacgca tcttatccga cctacacctt tggtgttact
    12841 cgaggcgatt ttttaacatt cccataagtt acacttattt aaagcgtcgt gaatttaatg
    12901 acgtaaattc ctgctattta ttcgtttgct gaagcgattt cgcagcattt gacgtcaccg
    12961 cttttacgtg gctttataaa agacgacgaa aagcaaagcc cgagcatatt cgcgccaatg
    13021 cgacgtgaag gatacagggc tatcaaacga taagatgggg tgtctggggt aatatgaacg
    13081 aacaatattc cgcattgcgt agtaatgtca gtatgctcgg caaagtgctg ggagaaacca
    13141 tcaaggatgc gttaggagaa cacattcttg aacgcgtaga aactatccgt aagttgtcga
    13201 aatcttcacg cgctggcaat gatgctaacc gccaggagtt gctcaccacc ttacaaaatt
    13261 tgtcgaacga cgagctgctg cccgttgcgc gtgcgtttag tcaattcctg aacctggcca
    13321 acaccgccga gcaataccac agcatttcgc cgaaaggcga agctgccagc aacccggaag
    13381 tgatcgcccg caccctgcgt aaactgaaaa accagccgga actgagcgaa gacaccatca
    13441 aaaaagcagt ggaatcgctg tcgctggaac tggtcctcac ggctcaccca accgaaatta
    13501 cccgtcgtac actgatccac aaaatggtgg aagtgaacgc ctgtttaaaa cagctcgata
    13561 acaaagatat cgccgactac gaacacaacc agctgatgcg ccgcctgcgc cagttgatcg
    13621 cccagtcatg gcataccgat gaaatccgta aactgcgtcc aagcccggta gatgaagcca
    13681 aatggggctt tgccgtagta gaaaacagcc tgtggcaagg cgtaccgaat tacctgcgcg
    13741 aactgaacga acaactggaa gagaatctcg gctacaaact gcccgtcgaa tttgttccgg
    13801 tccgttttac ctcgtggatg ggcggtgacc gcgacggcaa cccgaacgtc actgccgata
    13861 tcacccgcca cgtcctgctg ctcagtcgct ggaaagccac cgatctgttc ctgaaagata
    13921 ttcaggtgct ggtttctgaa ctgtcgatgg ttgaagcgac ccctgaactg ctggcgctgg
    13981 ttggcgaaga aggtgccgca gaaccgtatc gctatctgat gaaaaacctg cgttctcgcc
    14041 tgatggcgac acaggcatgg ctggaagcgc gcctgaaagg cgaagaactg ccaaaaccag
    14101 aaggcctgct gacacaaaac gaagaacttt gggaaccgct ctacgcttgc taccagtcac
    14161 ttcaggcgtg tggcatgggt attatcgcca acggcgatct gctcgacacc ctgcgccgcg
    14221 tgaaatgttt cggcgtaccg ctggtccgta ttgatatccg tcaggagagc acccgccata
    14281 ccgaagcgct gggcgagctg acccgctacc tcggtatcgg cgactacgaa agctggtcag
    14341 aggccgacaa acaggcgttc ctgatccgcg aactgaactc caaacgtccg cttctgccgc
    14401 gcaactggca accaagcgcc gaaacgcgcg aagtgctcga tacctgccag gtgattgccg
    14461 aagcgccgca aggctccatt gccgcctacg tgatctcgat ggcgaaaacg ccgtccgacg
    14521 tgctggctgt ccacctgctg ctgaaagaag cgggtatcgg gtttgcaatg ccggttgctc
    14581 cgctgtttga aaccctcgat gacctgaaca acgccaacga tgtcatgacc cagttgctga
    14641 atatcgactg gtatcgcggc ctgattcagg gcaaacagat ggtgatgatt ggctattccg
    14701 actcagcaaa agatgcgggc gtgatggcgg cttcctgggc gcaatatcag gcacaggatg
    14761 cattaatcaa aacctgcgaa aaagcgggta ttgaactgac gttgttccac ggtcgcggcg
    14821 gttccattgg tcgcggcggc gcacctgctc atgcggcgct gctgtcacaa ccgccaggaa
    14881 gcctgaaagg cggcctgcgc gtgactgaac agggcgagat gatccgcttt aaatatggtc
    14941 tgccagaaat caccgtcagc agcctgtcgc tgtacaccgg ggcgattttg gaagccaacc
    15001 tgctgccacc acctgagccg aaagagagct ggcgtcgcat tatggatgaa ctgtcagtca
    15061 tctcctgcga tctctaccgt ggctacgtac gtgaaaacaa agattttgtg ccttacttcc
    15121 gctccgctac gccggaacaa gaactgggca aactgccgct gggttcacgt ccggccaaac
    15181 gtcgcccgac cggtggcgtt gagtcactgc gcgctattcc gtggattttt gcctggacac
    15241 aaaaccgcct gatgctcccc gcctggctgg gtgcaggtac ggcgctgcaa aaagtggtcg
    15301 aagacggcaa acagagcgag ctggaagcca tgtgccgcga ttggccattc ttctcgacgc
    15361 gtctcggcat gctggagatg gtcttcgcca aagcagacct gtggctggcg gaatactatg
    15421 accaacgcct ggtagacaaa gcactgtggc cgttaggtaa agagttacgc aacctgcaag
    15481 aagaagacat caaagtggtg ctggcgattg ccaacgattc ccatctgatg gccgatctgc
    15541 cgtggattgc agagtctatt cagctacgga atatttacac cgacccgctg aacgtattgc
    15601 aggccgagtt gctgcaccgc tcccgccagg cagaaaaaga aggccatgag ccggatcctc
    15661 gcgtcgagca ggcgttaatg gtcactattg ccgggattgc ggcaggcatg cgtaataccg
    15721 gctaatcttc ccctcctgca aaccctcgtg cttttgcgcg agggttttct gaaatacttc
    15781 tgttctaaca ccctcgtttt caatatattt ctgtctgcat tttattcaaa ttctggatat
    15841 cccttcagat atccttaagg aattgtcgtt acattcggcg atattttttt caagacaggt
    15901 tcttactatg cattccacag aagtccaggc taaacccctt tttagctgga aagccctggg
    15961 ttgggcactg ctctactttt ggtttttctc tactctgcta caggccatta tttacatcag
    16021 tggttatagt ggcactaatg gcattcgcga ctcgctgtta ttcagttcgc tgtggttgat
    16081 cccggtattc ctctttccga agcggattaa aattattgcc gcagtaatcg gcgtggtgct
    16141 atgggcggcc tctctggcgg cgctgtgcta ctacgtcatc tacggtcagg agttctcgca
    16201 gagcgttctg tttgtgatgt tcgaaaccaa caccaacgaa gccagcgagt atttaagcca
    16261 gtatttcagc ctgaaaattg tgcttatcgc gctggcctat acggcagtgg cagttctgct
    16321 gtggacacgc ctgcgcccgg tctatattcc aaagccgtgg cgttatgttg tctcttttgc
    16381 cctactttat ggcttgattc tgcatccgat cgccatgaat acgtttatca aaaacaagcc
    16441 atttgagaaa acgttggata acctggcatc gcgtatggag cctgccgcac cgtggcaatt
    16501 cctgaccggc tattatcagt atcgtcagca actaaactcg ctaacaaagc tactgaatga
    16561 aaataatgcc ttgccgccgc tggctaattt caaagatgaa tcgggtaacg aaccgcgcac
    16621 cttagtgctg gtaattggcg aatcgaccca gcgtggacgc atgagtctgt acggttatcc
    16681 gcgtgaaacc acgccggagc tggatgcact gcataaaacc gatccgaatc tgaccgtgtt
    16741 taataacgtg gtgacgtctc gtccgtacac cattgaaatc ctgcaacagg cgctgacctt
    16801 tgccaatgaa aagaacccgg acctgtacct gacacagccg tcgctgatga acatgatgaa
    16861 acaggcgggt tataaaacct tctggatcac caaccagcag acgatgaccg cccgcaatac
    16921 catgctgacg gtattttcgc gccagaccga caagcagtac tacatgaacc agcaacgtac
    16981 gcagagtgcg cgtgaatacg acaccaacgt gctgaagccg ttccaggaag tgctgaatga
    17041 ccctgcgccg aagaaactga tcatcgttca tctgctgggt acgcatatca aatacaaata
    17101 ccgctacccg gaaaatcagg gcaagtttga tggcaatacc gatcatgttc cgccgggatt
    17161 aaacgcggaa gagctggagt catataacga ttatgacaac gctaacctgt ataacgatca
    17221 tgtggttgcc agcctgatta aagactttaa agcagcagac ccgaacggtt tcctggttta
    17281 cttctctgac cacggtgaag aggtttacga cacgccgccg cacaaaactc aggggcgtaa
    17341 cgaagacaac ccgacgcgtc atatgtacac cattccgttc ctgctgtgga cgtcggaaaa
    17401 atggcaagcg gctcatccgc gtgatttctc acaggatgtt gatcgtaaat acagccttgc
    17461 ggaactgatc cacacctggt cagatttggc gggcttatct tacgacggtt acgatccaac
    17521 ccgttcagtg gtgaatccgc agttcaaaga aactacccgc tggattggta acccgtacaa
    17581 gaaaaacgcg ctgatcgatt acgacacgct gccatatggc gaccaggtag gtaatcagta
    17641 aatgaccttt gtatcgcgtc tggcgctatt tgccggatgc gatactgccg caatgtttac
    17701 atcgaactcc cctcaggcca cacttttcag gtggcctttt ttaataatgc cagttactgt
    17761 tttcccgtct acatacctaa gggaataaca tgtatcacga cgtcagctat ctgctctccc
    17821 gcctgatcaa cggcccgctg tccctgcgcc agatttactt tgccagcagt aacggcccgg
    17881 tgcccgatct tgcgtatcag gtcgattttc cacggctgga aattgtgctg gaaggtgagt
    17941 ttgttgatac cggtgctgga gcaacgttag ttcccggcga tgtgttgtac gttcctgctg
    18001 gtggctggaa ttttccacaa tggcaagccc ccgccactac ctttagcgtg ctgtttggca
    18061 aacagcaact cggcttcagc gtcgtgcaat gggatggcaa acaatatcaa aatctggcga
    18121 agcaacacgt cgcccggcgc ggcccacgca taggttcttt tctgctacaa acgctcaatg
    18181 aaatgcaaat gcagccgcag gagcagcaaa cggcaaggct tatcgtcgcc agcctgctta
    18241 gccactgccg cgatttgctc ggcagccaaa tccagaccgc ctcacgcagc caggcactat
    18301 ttgaagctat tcgcgattat atcgacgaac gctatgcctc cgcgcttacc cgcgaatctg
    18361 ttgcacaggc gttttatatt tcaccaaatt acctctcgca cctgtttcaa aaaacggggg
    18421 ccattggttt taacgaatac ctgaatcaca cgcgactgga gcacgctaag acgttattga
    18481 aaggctatga cctgaaggta aaagaagtgg cgcacgcctg cggctttgtc gacagcaact
    18541 acttctgccg tctgtttcgt aaaaataccg aacgctcacc gtcggaatat cgtcggcagt
    18601 accacagcca actgacagaa aaaccgacta ctccagaatg agatgggttt gctgcggcgc
    18661 agaaagcact ttgcgcaccg cgctcatcac ccgctgcggc tcacgcagga atgcgtagat
    18721 gctgcattgc acatagcggc aatggtcaaa tcgctcagca ccggcaagct cgatatccgt
    18781 aattaacaga gcaacatccg cccgacggat atcctcgtcc gctaaacgat tctccgttcc
    18841 cagcgcgccc tgagtttcaa tgctgacgcc ccacttctct aacaggcaca acttttccag
    18901 ccgttccgcc gccatataag tatgcgccac gccgcttacg caggcggtta ctgccaccag
    18961 gtatgccatt ttaacctccc acggtaacct gaaatccggc ccgctctgcc atttcacgca
    19021 tcgttgccac atcgactgac gacggcgcag gcacctcttt catcgaccat gttttcccca
    19081 gcaggcggta tttcggttcg ccgtactgat gaaacggtaa cagatggatc tgcctgatat
    19141 tcagcgggat cagcacgtcc agcgcctgct gcatattctc ccggctgagc gtgaaaccag
    19201 ggatcagcgg taaacgcggg atcacgttga cgccctcact caccagcaaa cgcagattct
    19261 ccagcacgcg tggcaggttc atcttcacca catcccgcgc ctgagtcgcg tccataattt
    19321 ttaaatcgaa caacacttca tcgcacaatt tcgccagcgg taacagcttg ctggctggcg
    19381 cgtctccggc agtttcaatg gcgcatgaca cgccccacaa ccgcagccgc tgtaaaaaac
    19441 gggtagcaaa ctccgcctgc attaacactt cgccgccaga aagcgtgacg ccgccgccgg
    19501 acgtgcgaaa gaaaatgtca tctttcatca cttcccgttc cagcgcgtca aggctgatat
    19561 cgcggccaat ccgttcaaac gccccggagg ggcattcatc cgcatcacgc aaacatttcg
    19621 cacagtgcag acatttcgcc tctctgcgta ccgtctggat tttgccggag atcgactccg
    19681 gattagcaca ccatgggcaa agatgcggac agcctttaaa aaagaccacc gtacgaatgc
    19741 cctcgccgtc attcagtgaa taacgctgaa tgttgaaaat gcgcgccaca tcattgcggc
    19801 gcgtttccac aacgttacag ctgatgcgct gtccggcgga tgatgtcatc ctggatctcc
    19861 ttcgacaatt cgacaaagaa ggcgctgtat ccggcaacgc gcaccaccag cccggcataa
    19921 tcttgtgggc gctgttgcgc ttcccgcaac gtgtcggcgt tcaccacgtt aaactgaatg
    19981 tgttgtaact taagctgggt aaatgcccgt aagaagtcgg ccagtttgcg taatcccgct
    20041 tcaccttcca gggtcgccgg agtgaatttc acgttcagca acgtaccgtt agacagcagc
    20101 gtattatcga gcttactgac tgacttcagt accgccgttg gcccttgtgc gtcctggccc
    20161 agcatgggtg acaagccgcc gtctgccagt tgttcaccgg caaaacgacc gtctggcgtc
    20221 gcgccaacca ccgatcccaa cgggacgtga gcagaaacgg tatacgatcc cggcgtgaag
    20281 tagccgccgc gcgggttctg gtatttttcc acttctttgc agtagtggcg taacagttcg
    20341 gcgctgatgt tatccacctc gtcgatatcg ttaccgtatt tctcgaagcg gttaattaag
    20401 cgagcacgga ctttttcgcc ttctggcgtt gcgaagttgg cttttaatac cgacagcaat
    20461 tcgtcaaaac ttaaacgctg ttgatcaaaa atcatccctt tcaacgcatg gagagaatcg
    20521 ctcaggttgg cgataccgat cccctgtacg ccggagaagt tataacgcgc gccgccatcg
    20581 gtaatatcgc gacctttttc cagacaatcg ctgataaaag acgagagcag cggtacaggt
    20641 gcccagtcgc gatggccgat atcacaaata ttgctgccct ctaccatcag ggtgatgtag
    20701 tggctgatct tggcgcggat ctgttccagt aaaccttcat aagtcagcgc agcgttacct
    20761 tcattttcat gcaggcagat ttccatcact ttcagcaggt taaacatcgc gatgtcatgc
    20821 aagccgtagg ttctgccggg aatagataat tccacgcagc ccactacgga atagtcgcgc
    20881 gcatcttcca gcgaaacgcc acggttgagg aacgctggca ccaccacttc atcgttaaag
    20941 atttgcggaa taccggtacc gaggcgaata gtttctgcgg ttttcatcag gaacggcgtg
    21001 tcgatcaagg cgttagtgcg cacccccagg ttcggttgcg gtaattgcac actttgatag
    21061 gcgtcgagac agaggaacga aagtacgttc accgcgctac gtccgttctc ggttaacccg
    21121 ccgagcagtg cggtatagcc ggtcgggaaa ccggcgaaat aacgcgcgct actggtggag
    21181 cgcaacagca cgatatcgtt gcatttcacc cataaagatt cgagcagttc tttcaggaac
    21241 gccggatctt cgcactgggt taatgatgcc tgatagaacg gcaacatata ctggtcgaag
    21301 cgccctaaag acagagaact ggcgttggat tcgtattgca gaatgatgtt catgtaccag
    21361 aataactggc acgcctgcca gaacgtctgc ggcttatgtt cggcgttatg gcgggagatc
    21421 tccgcaatag tcagcagttc ttcgcgacgc tgggcatctg tgcagtttgc cgccatcgtt
    21481 tccgccagtt cagcgtaacg cagaatgtgt ttccgcgagg cttccagcag taacagcgct
    21541 gcctgataaa agtgattctc cggctgttgc tgacaatgtt gctgcatctg tgccaccagc
    21601 tcccccagcc cgtgattcag caggcgcggg taatcaataa taatgtgccc ctggccttta
    21661 tccgtctggt tgatgctgaa aatctgcgtg ctggtcgcgg cttttacttc atccgtcatc
    21721 tgcccgttga tgaaatcttt catcgaacgt ttttcccagt acgggaacaa ctcttcgcgg
    21781 tagatacgtt tatcttcttc gctgatagca aagcggtcct gcggacgcgt cgggaattga
    21841 tccaactctt tcagcagcca gtaagggtcc atttccggcg acataatccc ggcgcgcggt
    21901 tttacggtgc ggttaccggc aatcagttct tcatcacgaa tcgaaatttc aacatgttca
    21961 aggatatacg ctgttgcttt cgcccggcgc aatatcaccg gttcgccttc ggtttgccga
    22021 tggctggcgg tataaagcag cgcacgctcc agcgagattt cacgggtatt ggcaaacagt
    22081 gcagttttga ggcgagagat acgattcgtc atgaggaact cctgtgggaa agcgaacccg
    22141 cccttacggg cgggataaga acttaaacgg tttgtgccag gtgagcttca attttgctca
    22201 taatggcatc agcgcgtttt accgcgtcgc tgatgttgac gcgcacgatg gtcttgccag
    22261 caaaacgctc ttcaaacttg atgccgatat ctttggtcag aatcaccata tcggcgctcg
    22321 ccacgtcttc cgctgtcagt tcattttcca gaccaattga cccctgggtt tctactttca
    22381 cttcccagcc tttcgctttc gcggcacttt ccagcgcctc tgccgccata taggtatggg
    22441 caacaccaga tggacacgcg gttactgcaa taattttcgt catggctcgt tcctcaatta
    22501 attaatttca aaatccaaat ccaggtcgtc ttctttttca tcagtcgaag acccattttt
    22561 acgcgccaga cttttcagca cgttcacgca gactgccgtc accactgctc cgacagccac
    22621 tgctgcgata taacccagct tgccttcaac caccggcagc acaatcagac caccccaacc
    22681 tgcatagcac tgtgcaccga ccagtgccgc agttaccgca ccacaaactg aaccgaccat
    22741 gatggaagga ataacacgca gcggatcggc agcggcgaaa ggaatcgccc cttcagtaac
    22801 cccaacgcac cccatcacca gcgccgcttt accggtttcg cgctcttctg cggagaaatt
    22861 tttacgacca atcagcgtcg ccagccccat ccccagcggc ggaatacaaa tacccacggc
    22921 agcaatggcc accacggtat aaacaccctg agcaacgcaa atcagcatga aggcataggc
    22981 cactttgtta accggaccgc ccatatcgaa cgccagcatc aggcccatga tcaccgccag
    23041 cataacaatg ctgccctgct gcatcccctg aagccactga gtcaggctgt tggtcaatgc
    23101 ccccacaggc tcgcccagcc cccacatcat gatgcctgcg gtaatcagtg tgccgacgat
    23161 cggaatgatg aagattggca tcaccgaacg cagaacttta tgcaccggaa ttttcttcag
    23221 gtaatgcacc acgatgccgc cgataatccc ggcgatcagt gcaccaaaga agcccgcacc
    23281 aaagctgtta ccaacccagg caccgatagc gcatggagcc agcgcagaac gttctgcaat
    23341 ggagtaaccg atgtaagcga cgaggaaagg caccatcagc gtcaaacctg caacgccgat
    23401 atcaaacagt tttttcagat tcggatcggc gacggcatcc ggcactgcac ctttgccata
    23461 caacatgacg gaaaccgcca gcaaaatacc gcccgatacc acgaagggaa tcatgtgtga
    23521 aacgcccgtc attaaatgct gacgggtatt tttcaggatc tgcaccaact cattcataag
    23581 ctgctcccgg gcttgcaaaa gcccatgtcc atatttttga tggaggaaac aataggcaat
    23641 cgcaggcgga gcaaacagtg gataaaaagg ccatttactg gacttttgta atatcagtac
    23701 aaaaatgcga tccgcctcat aacttgcgat aatccgggag catgccgcaa aacggcgtct
    23761 tgtgagacgc gtcgcaaatt gacggttaca ttacatttgt ccagcttttg gcagatttgt
    23821 cacatggcag gccgttgccg tgcgctctta tcctgtaaac agaaaaccgt accgggagag
    23881 aggcaatggc cctgattgtg gaatttattt gtgagctacc taacggcgta catgcgcgtc
    23941 cggcaagcca cgttgaaacg ctgtgtaata ctttttcatc acaaattgag tggcataacc
    24001 tgcgcactga ccgtaagggc aacgccaaaa gcgctcttgc gctgattggc accgatacgc
    24061 tggtgggcga taactgccag ttactgattt ccggggccga cgaacaggaa gcgtaccagc
    24121 gtttaagcca atggctgcgc gatgaattcc cccactgcga tgcgccgctg gtggaagtta
    24181 aatctgacga actggaaccc ctgccggttt cactgaccaa tctgaatccg caaattatcc
    24241 gcgcccgcac cgtgtgcagc ggtagcgcag gcggcattct gacgccgatc tcttctttag
    24301 atctcaatgc gctgggtaat cttcctgctg ccaaaggcgt tgacgccgag caatccgcgc
    24361 tggaaaacgg cctgacactg gtgatgaaaa acattgaatt tcgtctgctg gatagcgacg
    24421 gtgctaccag cgcgatactg gaagctcacc gatccctggc tggcgatact tctttacgtc
    24481 agcacttact ggcaggcgtc agcgagggat taagctgtgc cgaagcgatt gtcgccagtg
    24541 cacaccactt ctgcgaagag ttcgctcgtt ccagcagcag ctacctgcaa gaacgtgccc
    24601 tggacgtacg cgacgtctgc ttccagttac tccagcaaat ctacggtgag caacgcttcc
    24661 cggcaccggg caaactgacg cagcccgcca tttgtatggc tgatgaactg acccccagcc
    24721 agttcctcga actggataaa aatcacctca aaggattgtt gctcaaaagc ggcggcacca
    24781 cctcacatac ggtgatcctt gcccgttcgt tcaacattcc gacgctggtt ggtgtggata
    24841 ttgatgccct cactccgtgg cagcatcaaa cgatttatat cgacggcaac gctggggcga
    24901 ttgtggttga gccaggggaa gccgtagctc gttattatca gcaggaagcc cgcgtacagg
    24961 acgccctgcg tgagcaacag cgtgtctggc tgacccaaca agcccgtacc gctgacggta
    25021 tccgcattga aattgccgct aacatcgctc actccgtgga agcgcaggcc gcattcggca
    25081 atggtgcgga aggcgttggt ttattccgca ctgaaatgct ctatatggat cgcaccagtg
    25141 caccgggcga aagcgagctg tacaacattt tttgtcaggc gctggagtcc gccaacggac
    25201 gcagcattat tgtgcgcact atggatattg gcggtgacaa acccgttgat tatctgaaca
    25261 ttcccgcaga ggcaaacccg ttcctcggtt atcgcgccgt gcgtatttat gaagagtacg
    25321 cgtcgttatt taccacacaa ctacggtcga ttctccgtgc ctccgctcac ggcagcctga
    25381 aaatcatgat cccgatgatc tcttcaatgg aagagatctt atgggtgaaa gaaaaactgg
    25441 cggaagccaa acagcaactg cgtaacgaac acattccgtt tgatgagaag atccagctcg
    25501 gcattatgct ggaagtgccg tcggtgatgt tcatcatcga tcaatgctgc gaagagattg
    25561 atttctttag tatcggtagt aatgacctga cgcaatatct gctggcagtg gatcgcgata
    25621 acgctaaggt tactcgtcac tataacagcc tgaatccagc attcttgcgg gcgctcgatt
    25681 acgccgtgca ggcggtgcat cgccagggca aatggattgg tctgtgcggt gaactgggag
    25741 cgaaaggttc cgtgctgcca ttgctggtcg gtttagggct ggatgagctg agcatgagcg
    25801 caccgtcaat tccggcggcg aaagcgcgaa tggcgcaact tgatagccgt gagtgccgca
    25861 agttgctcaa ccaggcaatg gcctgccgta cttcgctgga agtggaacat ctgctggcgc
    25921 aattccgcat gacccaacaa gacgcaccgc tggtcaccgc cgagtgcatc acactggaaa
    25981 gcgactggcg cagcaaagaa gaagtgctca aaggcatgac cgataacctg ctgctggcgg
    26041 gccgctgccg ctatccgcgt aaactggaag ccgacttgtg ggcgcgcgag gccgttttct
    26101 ctaccggtct gggctttagt tttgccattc cccacagcaa atcggaacat attgagcaat
    26161 ccaccatcag cgtagcgcgt ctgcaagcgc cggtgcgctg gggcgatgat gaagcgcaat
    26221 tcatcattat gttaacgctg aacaaacacg ctgcgggcga tcagcacatg cgcattttct
    26281 cgcgcctcgc tcgccgcatc atgcacgaag aattccgtaa cgcgctggtt aacgccgcct
    26341 ctgccgacgc tatcgccagc ctgctgcaac atgaactgga actgtaaaag gaaacatcat
    26401 ggaactgtat ctggacaccg ctaacgtcgc agaagtcgaa cgtctggcac gcatattccc
    26461 gattgccggg gtgacaacta acccgagcat tatcgctgcc agcaaggagt ccatatggga
    26521 agtgctgccg cgtctgcaaa aagcgattgg tgatgagggc attctgtttg ctcagaccat
    26581 gagccgcgac gcgcagggga tggtggaaga agcgaagcgc ctgcgcgacg ccattccggg
    26641 tattgtggtg aaaatcccgg tgacttccga aggtctggca gcaattaaaa tactgaaaaa
    26701 agagggtatt actacacttg gcaccgctgt atatagcgcc gcacaagggt tattagccgc
    26761 actggcaggg gcaaaatacg ttgctccgta tgttaaccgc gtagatgccc agggcggaga
    26821 cggcattcgt acggttcagg agctgcaagc actgttagaa atgcacgcac cagaaagcat
    26881 ggtgctggcg gccagcttta aaacgccgcg tcaggcgctg gactgtttac tggcaggatg
    26941 tgaatccatc accctgccct tagatgtagc gcaacaaatg ctcaacaccc ctgcggtaga
    27001 gtcagctata gagaagttcg aacacgactg gaatgccgca tttggcacta ctcatctcta
    27061 aaggagcaat tatggaccgc attattcaat caccgggtaa atacatccag ggcgctgatg
    27121 tgattaatcg tctgggcgaa tacctgaagc cgctggcaga acgctggtta gtggtgggtg
    27181 acaaatttgt tttaggtttt gctcaatcca ctgtcgagaa aagctttaaa gatgctggac
    27241 tggtagtaga aattgcgccg tttggcggtg aatgttcgca aaatgagatc gaccgtctgc
    27301 gtggcatctc ggagactgcg cagtgtggcg caattctcgg tatcggtggc ggaaaaactc
    27361 tcgatactgc caaagcactg gcacatttca tgggtgttcc ggtagcgatc gcaccgacta
    27421 tcgcctctac cgacgcaccg tgcagcgcat tgtctgttat ctacaccgat gatggtgaat
    27481 ttgaccgcta tctgatgttg ccaaataacc cgaatatggt cattgtcgac accaaaatcg
    27541 tcgctggcgc acctgcgcgt ctgttagcgg cgggtatcgg cgatgcgctg gcaacctggt
    27601 ttgaagcgcg tgcctgctct cgtagcggcg cgaccaccat ggcgggcggc aagtgcaccc
    27661 aggcggcgct ggcactggct gaactgtgct acaacaccct gctggaagaa ggcgaaaaag
    27721 cgatgcttgc cgccgaacag catgtagtaa cccctgcgct ggaacgcgtg attgaagcga
    27781 acacctattt aagcggtgta ggctttgaaa gtggtggtct ggctgcggcg cacgcagtgc
    27841 ataacggcct gaccgctatt ccagatgcac atcactatta tcacggtgaa aaagtggcat
    27901 ttggtacgct gacgcagctg gttctggaaa atgcgccggt ggaggaaatc gaaaccgtag
    27961 ctgcacttag ccatgcggta ggtttgccaa taactctcgc tcaactggat attaaagaag
    28021 atgtcccggc gaaaatgcga attgtggcag aagcggcatg tgcagaaggt gaaaccatcc
    28081 acaacatgcc tggcggcgcg acgccagatc aggtttacgc tgcactgctg gtagccgacc
    28141 agtacggtca gcgtttcctg caagagtggg aataacctac tccaaactcc cggcttgtcc
    28201 gggagtttga gagtgcaggc aaagtggctc actcgtcact gcgggcatgt ttttccccct
    28261 cgccctttca gggagagggt cggagtgagg gtaaatattc gccccggagc tggcctgttc
    28321 ccctcaccct aaccctctcc ccaaagggga gaggggactg ttcgagcact ttttatacgt
    28381 tatcacccgt ctgaaactcc cacgttgttc aggaattttt tatccgcttc tggacatttt
    28441 ctctacagaa caatcgttag cccggaaggt cgaatctgtg actcttattt tcacggacga
    28501 accagatgaa agcttcactg gcgctggtca gcctgctaac ggcgtttacc agcaactcct
    28561 taaaatctcc ggccgttcca ccaaccgtgg tacaaatcca ggccaacacc aaccttgcca
    28621 tcgcagacgg tgccagacag caaatcggca gcacgctatt ttacgatccg gcgtatgtgc
    28681 agcttaccta tcctggcggt gatgtcccgc aagaacgcgg cgtatgttcc gatgtggtga
    28741 tccgcgcctt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa gatatggcga
    28801 agaattttgc tgagtacccg caaaaatggc agttaaagcg cccggacagc aacatcgatc
    28861 accgtcgggt gcctaatctg gaaacctggt ttagccgcca tgataaaaca cgccccacca
    28921 gcaagaaccc cagtgactat caagcgggcg atattgtctc ctggcgactg gataacgggc
    28981 tggcgcatat tggggtggtg tcacatggct ttgcccgcga cggtacgccg ctggtgatac
    29041 ataacattgg cgcaggcgcg caggaagaag acgtgctgtt tagctggcgg atggtggggc
    29101 actatcggta ttttgttaaa taagcgatcg ccccacatga agaaaaaatt cagatgtttt
    29161 ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc acaatcagca
    29221 caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag gaaaagagta
    29281 cgccgcaaac gggaacggcc agtgtgctta agctggcaat actggcaggc aagtttttca
    29341 atacaaacaa ccataagctc cacgccagcg ccgtcgccag aatcgcactg taggccagag
    29401 cccagaacac ggtgggctgc cagtcaattt cacgttgtgg caccagtaaa gcgaccgcac
    29461 tcatcaccag cgccgcataa agcatctgcc aggatgttaa cgacaataaa tccacgcgtg
    29521 gatggcgggc gtataggcgt ttcgcgacaa tcgcgctcgc cccccaacta acaccggaga
    29581 ggattgccag cagggcactt ttcatcgaag agaaatcgag ttgccacggc tgcaacacca
    29641 aaaataagcc gaaagcggca atcagtatcg cgaaatattg cccacgtcgc aggcgttcac
    29701 cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc aggatcgcca
    29761 ccttccccgc tcctccactg accagcgccc actgcgccag gccaaccatc ccgcaggttt
    29821 gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg cgaccacgta
    29881 ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg gtgaagtcga
    29941 aggcaccgat gtagctggtg acttgcttca tgaaaatcca gctataactc cagataagtg
    30001 tgagcacaac caggccactg attgccagtg ggttgctctt tcctgcggca gacatgagag
    30061 atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc agcgactgcc
    30121 tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg aggttcatca
    30181 ctttcaccca tgccgccacg aagtctttaa caaacttctc gtgggcatcg ctgctggcgt
    30241 aaacttcagc caccgcacgc aagacagagt tagagccaaa gacaagatcc gcacggctgg
    30301 ctgtgtattt cacttcgccg gtttcacggt cacggccttc gaacagctct ttcgattcgt
    30361 cggtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca ttgctcaata
    30421 cgcccacgca gtcagtgaag actccgtttt tgctgccatc gaagttggca cccagtacgc
    30481 gcatcccgcc caccagcgcg gtcatttccg gcgcagtcag cgtcagttgc tgtgctttat
    30541 caatcagcaa tgactcggtg gtggaaacgt ccagacgagc gcgatagtta cggaaaccgt
    30601 cagcaattgg ctccagcagt tcgaacatct caatgtcagt ctgatcctga cgcgcatcaa
    30661 cgcgacccgg cgcaaacggt acatgaatgc tcaaaccagc ggcgcttgcg gctttctcaa
    30721 cgccaaccac acctgccagc acgatgatat ccgccagcga ggctttacca gactctttct
    30781 gaattttctc cagaactggc agcgcacgaa cggctgcggc gttcacatcc cagtcgcgct
    30841 gtggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca ccacggaagg
    30901 tagaagcaga cgcccaggct accgatacca gctcgctgac agacagacca gaatccgcaa
    30961 tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc tgcggcagcg
    31021 gctcttgcca gatcagatct tctttcggta cttccggccc gatgtaacga gatttcggcc
    31081 ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg aacgcctgcg
    31141 gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc agcgtcaggt
    31201 cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc gggataattt
    31261 ccggtgcgtc taccgcttcg aactggattg cgccagccgg gctgcgggtc tgtacccact
    31321 catatttgaa caggttctcg aagaaatagt tgctccactg ggtcggcgtc tgggtccaga
    31381 ccacttccag accagaggtg atggcatctg cgccaacgcc gctgccgtaa gtgctcgccc
    31441 aacctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg tttgatgtcg
    31501 gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc acggtttctt
    31561 cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca gaaagcggtt
    31621 cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg gttgcgccca
    31681 gcggtgcttt cgccagtgct tccggatgac ggtgggtcag ccaggctttt tcatcacccc
    31741 agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa ccgaaggtac
    31801 ggaagccgga gttttccagc gccacgttac ccgcgaggat aaacaggtcg gcccaggaga
    31861 ttttctgacc atatttctgt ttgattggcc acagcaggcg acgcgcttta tcgaggctta
    31921 cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc gcgccgccgc
    31981 gtccgtcgat tgaacggtaa gtccccgcgc cgtgccaggc catacgaata aacagaccgg
    32041 catagctgcc ccagtcggca ggccaccacg gctgagattc tgtcagcagg gctttcagat
    32101 cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca aagtcctcac
    32161 ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt tgatttggcc
    32221 accagtcgcg cgtggttgtg cccccacctg cgctctggtc gtgaccgccc tgatggaacg
    32281 ggcatttgcc agtggctgtc gtgttatgga tatcgtctga cgtgctcatc aatgtgctcc
    32341 cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat tgaattaatc
    32401 cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag atcaaattga
    32461 tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata aaaaagggtt
    32521 ttagctttat atccttcaag taatgaggga gaaaacaagg ctgacaccct tattttcccg
    32581 ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact gtgtgatttt
    32641 cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca aatcgcgtaa
    32701 ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc acggcttaaa
    32761 atcttcacca tatccatcgc aatattcgcg cccaccagtt tgcgggtttc ggcatcgtca
    32821 tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt catgtcggca
    32881 aatttcttcg cctgtttaaa gttagatacc ggcaaaattc ccggaataat ttccacatca
    32941 atgcccgccg atacgcagcg gtcacgaaaa cgcaggtagc tttcgacatc aaagaagaac
    33001 tgagtaatcg cgcggttggc tccggcatcg actttgcgtt tcaggttgag caaatccgcc
    33061 tgagcgcttt tcgcttccgg atgaacttcc ggatacgccg ccacggagat atcgaaatct
    33121 gccacttctt ttaacagcgt caccaggtca gaagcgtaca tttctggctt accacttccc
    33181 ggcggcagat cgccacgcag cgccacgata tgacgaatac cgttattcca gtagtcacgt
    33241 gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg tgccgcttcc
    33301 agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg ctcgccggag
    33361 ttcgcgccat aggtcaccga cacaaacttc ggtttcaggt tgctaaggcg atcgatggag
    33421 ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa cgaaacgtta
    33481 atctgcccct ggacttctgc cagactctga ttcagggcat cccgctggct ggcgtgaaaa
    33541 aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg cacatctatc
    33601 cgtttagacg tccagatgta aagatgaagg aaaagctaaa gggcgtcaac cgaaaaatga
    33661 ccaatgatgg tgaggaaaaa tcagggaagt tgaaaaaaac tcatcttaat tagaggcgat
    33721 aaaagatttc tctatagcgc aatccacctc gcacgttttg actgatattc tgacaacaca
    33781 gcgacacctg ggcttattcg aaaaacaacg tcctgccacc ggatggtgat gctaaggcat
    33841 ctgattcggc ctgcgccctc gcgtaggccg aatactggat ctgcaaaatc aactggtaac
    33901 aaacagatga atcaactcaa tacgtacttt tacttgatat ctttcacgcg cataccgttg
    33961 atctgttcat ccgtgatggt gttacccgct ttgaagtaat caaccacggc gtgataaacg
    34021 taataaccgc ccgttgtgtt acgcgctttc ccttcggtaa aggcggtaaa accatctcca
    34081 ccatcagcaa ggaaactctg agtagcaatg tggtaaatcg tcgcatcttc aatcggtttg
    34141 ccattcagag tcagcgtgat tacccgctga ccaaccggct tactgctgtc gtacttcatt
    34201 tccaggcctt tcgatacctg taaaacgcca ttacttaaac ttgcgccgtg ttccatcaaa
    34261 ctgcgtaatt gtttacccgt gagatccatc gtcaccagtt cgttgggaaa cgggaaggta
    34321 ctgatgacgc cgcccatcgt aatcgcccct gcggggatct cattgcgaat gccgccagag
    34381 ttggttaacg ccaattgggt gtttttaccc gccgctacca gtaaagcatc agccgccagg
    34441 ttcccgaggg aagccgattc accataggca cgttttaatt caaccggcga tttcgcgacc
    34501 gtttgttgca ccacttcatc aagcttttta ttccagccgt cgatcacctg ttttgtttgc
    34561 ggatcgggct tccactcgtc ggcgtaaatg gttttaagct cgaagttttt caccgtaaaa
    34621 tcatgcggct tctctttgta gtcgagaacc agtttgccca catcaatccc gccgctgtca
    34681 gtggagagga tgagcgtatt gccgacttta atcggttccg gcgtacccac atgcgcatgt
    34741 ccggtgatca aaatatccag ccctttcacc tgacttgccg tctgaatatc tttatccagc
    34801 gcgcgacgca catctgtgcc tcccatactg gactggcggg ccggaacacc ttcatggatc
    34861 agggcgacgg tcaaatcaac cttgccttta agttcatcga tataacgttg tagccattta
    34921 atttcatcac gcgcttcaat ccccactcgc gttgccgcag ataccgtgtc attaaaggca
    34981 aatacaccgt gcaaacctat aacgcctatt ttcacaccgt ctttttcgat gatggtatat
    35041 ggcttatccc agaatgattt actgctgttc tgataaaaaa cattgccctg cacaataggg
    35101 aattttgcct gactcaactg taataatgtg ttgtcccagc cgtggtcaaa ttcatggttg
    35161 cctatcgtga cggcatcaaa cggcatagta ttcataatat cgataatagc cttacctttg
    35221 gtcaggctgc tgatatatgg cccggtaaaa taatcacccg catcaaaaaa ccatgtcgct
    35281 ttattttttg ctttttcttg cttaaccagc gtagtgatat ttgcccagcc gccaatatcc
    35341 cgtttaccat cagcgatcca cggcacttta taaggctcga catgcgcatg gaggtcgttg
    35401 gtataaatga tggtgacatc tttggcgcaa gcccagaacg gcaatgttaa tgcgataccc
    35461 gcagcaagta ttttaatttt cataaatagt ccctttcagt gatttaaagg attgctacat
    35521 tacgttctga aagagttatt tatgtgagag taatcacatc cgcaggggat agttgccgga
    35581 caaaatcaac gaccttgtga aatcgttggt gatatatgtc gactctgttt acgattaatg
    35641 cctgcaagtc gtttggttgt cgtaatttag ggcaaccaac atcgacagat tacagctggc
    35701 cagactatcg actgggttat ccggcactgc actgtcgggc atgtggaagt tacccgccgt
    35761 tgtttgacga acagcaattt cgtgactggt tgtcagttca cttatctact tttgcaactg
    35821 agaaaggtca tttctgcccg gtatgttatg gcacaaagac gatttgttat ggccacaatc
    35881 cacaggggtc gcaacgcatt caatgtcgta actgcaaaaa agtctggaca cctaaacaat
    35941 atcagaaaga aataacgcct cctgaaataa tcgaaacggt tgctcttctg gtgccatttc
    36001 agggagccag cggcgggcag aaactgtatg ttttaatcag ttttgatgct cttcgcggta
    36061 atattcttca tctttctact aattacacgc agcatcaggc tggagaaagt ctgcattatc
    36121 gatacagagg gaatgcagaa ccagagttac acgagagtaa tattgtacaa cgcgttgata
    36181 tgcgcgaagc acaatttcta cgccgcagtc agtttgatga gattcaatat ggcagcgcag
    36241 cgctcaaacg caatgctaaa ggtgttattc tgcgtccggt tatcactgca cacgggcatt
    36301 tcagagtact gaatatttta ttccctacag taaaaacaca tgtaatttca cacgagtgtt
    36361 ttttaagagg tgccattatt acggcatggg ctgatctgtt tcgccaacag caaggtgaga
    36421 tttggtttat tgaagaggaa atagctgacg ataccgataa tatgccctgg cgttttcagg
    36481 gaacaactta tcacggctgg tggaaaaatc agtggcaact ctgggttcag ggcaaaaatc
    36541 gtaaaatggt atgtgcatta acgggcggaa ataacagcaa agcagaaatg ctctctttag
    36601 caaccagcag acattttatc gactggctac ataaacaagc agtatttact cacagcgccc
    36661 cgctttccgc agggcgcgtt acgcaaatac tgctttctct ggctcaggat tacaacaact
    36721 gcgccaggcg gttaatatcc gactgaatcg cccctgcggt gacgtcgcgc cccgcgccag
    36781 gcccacggat caccagaggg ttatcgcgat accagcggct ttcgatggca aagacgttat
    36841 cgcacggcag cagtgatgcc agcggatggt cttcacgcac cgcttccacg cccacacgcg
    36901 ctttgccatt ggcatcgaaa cgcgccacat agcgcagcac cagccccatt tcgcgggccg
    36961 cttccagtcg ttgcaccatc tgctcgttaa gttcatcgcc gttttcaaag aaatggtcga
    37021 tgctaccgcc ttcgcaatgg gcaggcacca gcgattccac gcgcacctga tctggttcga
    37081 tgtcgtaacc cgcttcacgc gccagaatca ccagcttgcg catcacgtct ttgccggaaa
    37141 ggtcatcacg cggatcaggc tcggttaagc cctgctgcca cgcctgatcc accagctcgg
    37201 taaacggcac gctaccgtcg aattgcagga acagccagga gagcgtgccg gagaagatcc
    37261 cactgatcga caaaatagta tcgccgctgt cgatcagatc gcgcacggta tggttgatcg
    37321 gcaagcccgc accgacggtg gcgttgtaca gccagtgacg cccggttttt tcgaaggcgt
    37381 cgtggatctg gcgatattta ttgctgtcgc tcgctcccgc cagtttgttg gcgctgatga
    37441 cgtggaaacc gtggctggca aaatcgagat actgatcagc aagctgctgg ctggcggtaa
    37501 cgtccaacac cactaaatca tcatacggat gggcgcgcat ccacaggaac agcgactctt
    37561 catcctgctc gaccgcttca tcgttgaaga aggctaacgc gcggctggcg tccagcccgt
    37621 catagctcaa caggctgcgg cggctgtcca ccacgcctgc cagcacaaat tcaaagccgg
    37681 tacgcgccga aagcgtgctc tgctcacggg cgaacagttc cagccagcgg gaaccgatat
    37741 tgcccttgcc gaacaatacc aggccgatgc gtttttctgc gcggaagacg gactgatgca
    37801 gcccctgaat caggctttcg gttggtccgg tgcgcagtac tgccaccagg ctgatgccgt
    37861 catcggactg ccaggtaaat tcgaccggct ggcctttcag ttgctgccag aagcggtggc
    37921 aatgcagcgg gttacgggtg acacccgccc caaccatcgc caccagcgcc agcccctgac
    37981 gcaggcgcag ttcgccaggt aatccctctt cgtcgaggat tttcagcgca ctgtcggcca
    38041 cttctgaggt gtagcaaaat tgcagcaact ggcgatcgtt atgcacgcca accgccagtg
    38101 ggcgcacctg agcacgtttc aggatctggt cgatctcttt atgcgccagt ttgaaatcct
    38161 ggctggcggg cacctgaaac tcaatcaagc agacatcatc atggctggtg acaatacgcg
    38221 caccagtacc ggaggccagc acgcgttcaa tgcgcgtgga accttgatcc ggcgtgtagc
    38281 tacagcgcag ttgcaggtcg atttcgctgc cagaaactgg ctgtaaagta cgggcgtgaa
    38341 gaacgggagc cgccagacgc gccagttcgc tggcctcatc cagacgcagc aacggcagca
    38401 ggcaggcatc tttcacttta cgcgggtcgg cgctgtatac cccggcgacg tcgctccaga
    38461 tggttacgcg ggaaactccc gccagcgcac cgatttgtgt cgcggaatag tcggaaccgt
    38521 tacgccccag cagcaccgtt tcaccggcgt tgttacggct gataaacccg gtcaccacca
    38581 gacgtttgcc tggatgttgt accagtagct gttgcagcaa cgggtaagaa agcccttcat
    38641 caacctgcgg ttgcgcagcg cgttcagcgc gtaaaaactc gcgagcatca agccaggccg
    38701 ctggcagccc ttgttgatta agtaccgcag acatcaggcg cgccgaccac acttcgccgt
    38761 ggcccaccac ttccgcatac accgcgtcgt taataccgct gtcgagcagc gccgccaggc
    38821 gctcaagatc gctgataaag gcactaatga ggctatcggc ttcttcggcg ggtagcaggc
    38881 cgctaatcag atcgcactga tagcgacgca gcgtttgttg aacctgatgc gcagagagac
    38941 gatcggtctg acttagtttc aaccagttaa tcaactggtt agtggtgcta ccggcggcgg
    39001 aaaccaccat catatcgtca ggctgagagt actccgccat aatgcccgcg acacgcaaat
    39061 aacacttcac atcagccaga ctactgccac caaatttatg cagttgacga cctttcgccc
    39121 ctgcctgcgc aatcacactc atttttaccc cttgtttgca gcccggaagc cattttccag
    39181 gtcagcaatt aaatcttcgc catcttcaat accggtggag atacgcagca gcgtctcgga
    39241 gatcccggcg gcagcacgcg cttctggtgc catgcctgca tgtgtcatgg ttgcggcgtg
    39301 agagattaaa ctttccactc cccctaatga ttccgccagc gtaaacaacg acagcccgcc
    39361 caggaaacga cgcagcgtct gctcatcgcc atccagttca aaactcaaca ttgcgccaaa
    39421 gcctttttgc tggcgcgcgg caatttcatg cccctgattt tctggcagcg acgggtgata
    39481 cagttttttc accaacggct gggtttgcag atatttcaca atcgcctgcg cgttgcgctg
    39541 cgccagctcc atacgcggca ccagtgttcg caacccgcgt agcagcagat agctgtcaaa
    39601 cgcgccgccc gtcacgccaa tattgtttgc ccaccaggca agttcagtga caacgtccgg
    39661 gtctttagca atcaccacgc cggccaccac gtctgagtga ccgttcagat atttcgtaca
    39721 tgaatgcaac accagatcgg cacctaatgc cagcggattt tgtaatgccg ggcttaagaa
    39781 ggtgttatcc accacgctca ccgccccgac ttcccttgcc agatggcaga ttttcgcaat
    39841 atccacgacg cgtaacaatg gattacttgg gctttctacc agtaccagtt tgggtttttc
    39901 tgccagcgct gcccgtaatg cctgttcatc gccttgatca acaaacaaca cgcgatagca
    39961 accacgttta gccaggctgt cgaacaggcg atagctgccg ccatagcagt cgtgcggcgc
    40021 aaccagcaga tcgcccggtt tcaaaaagac ggtcgttacc aggtgaatcg cggacatgcc
    40081 ggtattagta agtacggcac cggcaccgcc ttccagttct gccagcgcac gctgaaccac
    40141 atcgcgcgtt gggttgccac gacgcgagta atcatgcgcg cgcggttcat taaatccggt
    40201 aaagttatag gtgctggaaa gatggatcgg tgggacaacg caaccatact gttcgtcgtc
    40261 atttaacccg ctacgcactg cgatggtggc ctgtttacgc gtcatgtgat gaagttccct
    40321 gggcttggtc ggtgaaatgt caggcaccag agtaaacatt gtgttaatgg acgtcaatac
    40381 atctggacat ctaaacttct ttgcgtatag attgagcaaa tcccaaatag ccgttaaaat
    40441 tatatgcatt atcacgccga caggtgcatt acacgatgtc acggtaacgc ctgtacggta
    40501 aactatgcgg gtttacggtc agtacccaca ctaactgtgt ggtctggtct caatttattg
    40561 acgaagagga ttaagtatct catggctgaa tggagcggcg aatatatcag cccatacgct
    40621 gagcacggca agaagagtga acaagtcaaa aagattacgg tttccattcc tcttaaggtg
    40681 ttaaaaatcc tcaccgatga acgcacgcgt cgtcaggtga acaacctgcg tcacgctacc
    40741 aacagcgagc tgctgtgcga agcgtttctg catgccttta ccgggcaacc tttgccggat
    40801 gatgccgatc tgcgtaaaga gcgcagcgac gaaatcccgg aagcggcaaa agagatcatg
    40861 cgtgagatgg ggattaaccc ggagacgtgg gaatactaaa gcggaaaaga gcggcgcgga
    40921 gttaacctgc cgcttttttt atacatggac gtttaacgat gataatcagg ctactgatcc
    40981 ccggtctgct ggtttctgtg cctgcatttg cctggcaacc acaaaccggc gacatcatct
    41041 ttcagatctc tcgctcatcg caaagtaaag cgatccaact ggcgacccat tccgattaca
    41101 gccacaccgg tatgctggtg atgcgtaaca aaaagcccta cgtttttgaa gcggtcggcc
    41161 cggtgaaata caccccgctc aagcagtgga ttgcccatgg tgaaaagggc aaatacgttg
    41221 ttcgccgcgt tgaaggcgga ctgagtgtta aacaacagca aaaactggcg caaacggcaa
    41281 aacgttatct cggcaaaccg tacgatttca gcttctcgtg gagcgacgat cgccagtact
    41341 gttcggaagt ggtgtggaag gtttatcaga atgcgctggg aatgcgcgtg ggcgaacagc
    41401 agaagttgaa agagttcgac ctgagcagcc cacaagttca ggcaaagctc aaagaacgtt
    41461 acggcaagaa tattccgctt gaggaaacgg tggtctcgcc acaagccgtt ttcgatgcgc
    41521 cacaactcac cactgttgcc aaagaatggc cgctgttttc gtggtaatgg atgataaaaa
    41581 aaataattcc cgccgtagtc tgtttgttgc tttgtagttg cacgaccttt ccgcaagctg
    41641 tcgctccggt aaacggtgat gcctccaatt agttgaatta aggatataat actcgctttt
    41701 aaggttgttt catggtcaca gttaaccttc attgtccccg ttgccagtcc gttcaggttt
    41761 accgtcacgg gcagaacccc aaaagacatg atcgattccg cggccgtgac tgtcaccggg
    41821 tatttcagct cagttactgt tacgagcccc gtaaaccagg agttaaagac caaatcgctg
    41881 aaatggcttt caacggtgcc ggggttcgct atactgcccg tacgctgaaa attggcatta
    41941 atacagtcat ccgaacttta aaaaactctc gccacgaaaa ataacgtcat ccccggtcgc
    42001 tcatgctgat gtggcactca tctgcgaact tagtcagcaa tggagctttg tcggcagtaa
    42061 agcgcgacag cactggctct ggtacgtgta caacacgaaa actggtggtg ttctggccta
    42121 cacttttggc ccccggactg atgaaacctg tcgtgaactg ctggcgctgc ttaccctttt
    42181 accatcggca tgttgaccac tgacgaatgg ggtagttacg ctagagagtt accaaaggag
    42241 aagcatctga ctggtaagat ttttactcag cacattgagc gtaacaatct gcccctgaga
    42301 acccgaatca aacgtctggc ccgcagaacc atctgtttct cgcgttctgt agaacttcat
    42361 gaaaaagtca tcggtgcttt tatcgaaaat atatgttcta ctaattggag tcatcaccct
    42421 tctgatgagc tttccttgga tgattaccag actgaataca cagccaaaga tttttacctc
    42481 attctgctct tttttctcaa atgacatata ttatatttta catcatattc ataggcatat
    42541 gacattcccc cgccccccat tatgtcattg tgggttgatg ttgggctttc ataaaaaaat
    42601 acatcttttt ttgaacta
//
LOCUS       DAPOZO010000039        42398 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPOZO010000039 DAPOZO010000000
VERSION     DAPOZO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.039"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(18..1385)
                     /gene="ghxQ"
                     /locus_tag="ROI21_002184"
                     /gene_synonym="ygfQ"
     CDS             complement(18..1385)
                     /gene="ghxQ"
                     /locus_tag="ROI21_002184"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDX5111102.1"
                     /translation="MSGDILQTPDAPKSQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(1421..2737)
                     /gene="guaD"
                     /locus_tag="ROI21_002185"
     CDS             complement(1421..2737)
                     /gene="guaD"
                     /locus_tag="ROI21_002185"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDX5111103.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAKRLKEEYPDTWVHTHLCENKDEIAWVKELYPEHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(2755..4155)
                     /gene="xanQ"
                     /locus_tag="ROI21_002186"
     CDS             complement(2755..4155)
                     /gene="xanQ"
                     /locus_tag="ROI21_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDX5111104.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSEEEMD"
     gene            complement(4320..7190)
                     /locus_tag="ROI21_002187"
     CDS             complement(4320..7190)
                     /locus_tag="ROI21_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDX5111105.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(7187..7966)
                     /locus_tag="ROI21_002188"
     CDS             complement(7187..7966)
                     /locus_tag="ROI21_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDX5111106.1"
                     /translation="MIEQFFRPDSVEQALELKRRYKDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(8017..9345)
                     /gene="ssnA"
                     /locus_tag="ROI21_002189"
     CDS             complement(8017..9345)
                     /gene="ssnA"
                     /locus_tag="ROI21_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDX5111107.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(9348..12446)
                     /gene="ygfK"
                     /locus_tag="ROI21_002190"
     CDS             complement(9348..12446)
                     /gene="ygfK"
                     /locus_tag="ROI21_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDX5111108.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRECLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSVCMRELEGSDAWGLDHVDVERLNKLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDVILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVDSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(12768..13346)
                     /gene="mocA"
                     /locus_tag="ROI21_002191"
     CDS             complement(12768..13346)
                     /gene="mocA"
                     /locus_tag="ROI21_002191"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDX5111109.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVKTEYCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNITNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            13449..14219
                     /gene="yqeC"
                     /locus_tag="ROI21_002192"
     CDS             13449..14219
                     /gene="yqeC"
                     /locus_tag="ROI21_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDX5111110.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            14267..15892
                     /locus_tag="ROI21_002193"
     CDS             14267..15892
                     /locus_tag="ROI21_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDX5111111.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFI
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(15934..16866)
                     /gene="arcC"
                     /locus_tag="ROI21_002194"
     CDS             complement(15934..16866)
                     /gene="arcC"
                     /locus_tag="ROI21_002194"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDX5111112.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(16914..18299)
                     /gene="hyuA"
                     /locus_tag="ROI21_002195"
     CDS             complement(16914..18299)
                     /gene="hyuA"
                     /locus_tag="ROI21_002195"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDX5111113.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(18352..19563)
                     /locus_tag="ROI21_002196"
     CDS             complement(18352..19563)
                     /locus_tag="ROI21_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDX5111114.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(19621..20817)
                     /gene="dpaL"
                     /locus_tag="ROI21_002197"
     CDS             complement(19621..20817)
                     /gene="dpaL"
                     /locus_tag="ROI21_002197"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDX5111115.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(20875..22062)
                     /gene="ygeW"
                     /locus_tag="ROI21_002198"
     CDS             complement(20875..22062)
                     /gene="ygeW"
                     /locus_tag="ROI21_002198"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDX5111116.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            22541..24319
                     /locus_tag="ROI21_002199"
     CDS             22541..24319
                     /locus_tag="ROI21_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDX5111117.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDDNRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(24359..24838)
                     /gene="xdhC"
                     /locus_tag="ROI21_002200"
     CDS             complement(24359..24838)
                     /gene="xdhC"
                     /locus_tag="ROI21_002200"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDX5111118.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGMPLSELLRDQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(24835..25713)
                     /gene="xdhB"
                     /locus_tag="ROI21_002201"
     CDS             complement(24835..25713)
                     /gene="xdhB"
                     /locus_tag="ROI21_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDX5111119.1"
                     /translation="MFDFASYHRAATLADAINLLTDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(25724..28021)
                     /gene="xdhA"
                     /locus_tag="ROI21_002202"
     CDS             complement(25724..28021)
                     /gene="xdhA"
                     /locus_tag="ROI21_002202"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDX5111120.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAEPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALNIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDITPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTIDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            28437..29189
                     /locus_tag="ROI21_002203"
     CDS             28437..29189
                     /locus_tag="ROI21_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDX5111121.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            29268..29341
                     /locus_tag="ROI21_002204"
     tRNA            29268..29341
                     /locus_tag="ROI21_002204"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29300..29302,aa:Gly,seq:ccc)
     gene            29574..31184
                     /locus_tag="ROI21_002205"
     CDS             29574..31184
                     /locus_tag="ROI21_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111122.1"
                     /translation="MKGKSTLTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGISGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLISIALLVAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFISDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREMSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            31250..31429
                     /locus_tag="ROI21_002206"
     CDS             31250..31429
                     /locus_tag="ROI21_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HDX5111123.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAVITGLTWPL
                     SLIPSIISLMIRKSD"
     gene            31775..32524
                     /locus_tag="ROI21_002207"
     CDS             31775..32524
                     /locus_tag="ROI21_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5111124.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
     gene            32521..34224
                     /locus_tag="ROI21_002208"
     CDS             32521..34224
                     /locus_tag="ROI21_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HDX5111125.1"
                     /translation="MKIKLRITIILISALCIFNGLLTPGAYAATANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGGGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFASRNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            34229..35374
                     /locus_tag="ROI21_002209"
     CDS             34229..35374
                     /locus_tag="ROI21_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN/LcrE/InvE/MxiC type III secretion system
                     gatekeeper"
                     /protein_id="HDX5111126.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESELVVVLRAILRKKQISENVRLNAEALLKKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRILVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQSAEALFLKTLMNNPIIKKFIS
                     AEDYWIFFLISLIKFPETAEELLKNALVTLPADANYKDKTLLLKAIYSGCTNLPFSLF
                     INNEQLLEIRECCKQAIKVTFAAELFDTQNSNKKQNKKPWKKVMFNV"
     gene            35367..37427
                     /locus_tag="ROI21_002210"
     CDS             35367..37427
                     /locus_tag="ROI21_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HDX5111127.1"
                     /translation="MFNKVLIGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIIVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGVEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPIIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLAHNINEYFGIQETKYILD
                     QLEMKYPDLLKEILRYITVQRISEVIQRLIQERISVRNMRLVMEALALWSPREKDIIT
                     LVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEM
                     ILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNI
                     LKTI"
     gene            37459..38751
                     /gene="sctN"
                     /locus_tag="ROI21_002211"
     CDS             37459..38751
                     /gene="sctN"
                     /locus_tag="ROI21_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HDX5111128.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASWQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            38741..39199
                     /locus_tag="ROI21_002212"
     CDS             38741..39199
                     /locus_tag="ROI21_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDX5111129.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            39177..40052
                     /locus_tag="ROI21_002213"
     CDS             39177..40052
                     /locus_tag="ROI21_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDX5111130.1"
                     /translation="MDEVKKIEHCKILNTDTPDNSASDLDSFLKKNKKNKEMSEIVAG
                     TILQLLRQTQDSQFSTLGKKNGHMESIDNKSSLQVDRNPSLSLQTNGTEKFSTKKILN
                     MTSRDQGNDSEPSNKTIQFKEKIHNGIHTEYITDQKHSNNKDREKKYRDGDKINGPQA
                     HSLDITNERRFADNRTMFTQHIEKQRNVHTLNQNDINNSANNANVRENELTYQFQRWG
                     QNHTVRILESSEGIRLKPSDTLVSDRLHEAQHNDVTAQRWVLTEQDERQGQRHQPHDE
                     QENEGKFENNQKDES"
     gene            40052..40999
                     /locus_tag="ROI21_002214"
     CDS             40052..40999
                     /locus_tag="ROI21_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HDX5111131.1"
                     /translation="MFGLRKVNGNTHSFETTFQNWKENGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLRELFPLISAELAGMALMPETERLIVEWLSLSSTPLNLKYPELKYNRL
                     CVGKVFDGVLSPAQPLIRIWTGELNIWLDKVTVCQYENAPTLDKKSLYWPIHFVIGFS
                     KTCYRTIVDIEVGDVLLISNNFAYAVIYNTKICDLIYPEELKMVDHFEYEEDFETDDF
                     DIKKSEIEINDENDYQQINSFEDLPVKIEFVLGKKIMNLYEIDDLCAKRIISLLPEAE
                     KNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGNNNVK"
     gene            40989..41654
                     /locus_tag="ROI21_002215"
     CDS             40989..41654
                     /locus_tag="ROI21_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDX5111132.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            41664..41924
                     /locus_tag="ROI21_002216"
     CDS             41664..41924
                     /locus_tag="ROI21_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HDX5111133.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            41926..>42398
                     /gene="sctT"
                     /locus_tag="ROI21_002217"
     CDS             41926..>42398
                     /gene="sctT"
                     /locus_tag="ROI21_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDX5111134.1"
                     /translation="MGEAMLYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELSTLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWP"
BASE COUNT        11397 a         9492 c        10349 g        11160 t
ORIGIN      
        1 aaggcgcgga gaatctatta gattgcccaa ccacccgcgt aaaacgcgac cagtgcggca
       61 gtaatgatca ccgtaccaat attcagcttt tgccattcgc gtgcaaagac gcggcctacg
      121 accagtgtca caaagcccag cataataccg gtaacgatat tacaagtcag aacgatgaac
      181 acggcacaca ccaggccagc cattgcgtcg ataaaatcat tgaaatccag cttcgagacg
      241 ttacttaaca tcagcaaacc tacgtacatc agagcgggtg cagtggcgta accagggatc
      301 aaaaatgaca gcggtgataa gaacaaaatc agcaggaata acgcccccac tacggttgcg
      361 gtcaaccctg ttttaccccc ggcggcggtt cctgccgctg attcgatata aaccgctgcg
      421 ggcgctgcgc ccaccagacc ggagaatatt gaacttactg agtcactggt cagggctttg
      481 ccgccgttga tgatctggtt gtctttatcc aacaaattcg cctgaccggc gacggcacgg
      541 atggtgccag tggcgtcgaa cactgcggtc atcaccaatg ccagtacact cggaagtaca
      601 gttggctgga gtgcgcccat aatatcgagg ctgaaaatca gagacttacc atcttcgcca
      661 gtcaggcttg gcatcgccac cagaccgtgg tacttcaccg ctgggtcaaa gattaagccg
      721 atgatcgaaa ttgcaataat caccaacaag atcccgccgg gtacgcggca cttttccaga
      781 ccgaagatga ccgccagccc cagcaagctc atcatcaccg ggaaggaggt aaacgcaccg
      841 agcgccactg gcaagccttc aatcgggttt ttgataacca ttcccacacc gttagcagca
      901 atcagcagca gaaacaggcc gataccgata cctgtaccgt gagctatccc catcggtaaa
      961 ttacgtaaga tccaggtacg cacaccggtt acggaaatgg cggtgaagat gacgcccatc
     1021 agaaagaccg cgcccagtgc gacaggaacg ctaatttgtt gcccgagtac cagactgaat
     1081 gcggtaaacg ccgtcaggga aatcgcgcaa ccaatcgcca ttggcaaatt ggcccataac
     1141 cccatcagca acgagccgaa gcccgcgacc agacaggtgg caacaaacac agctgcggga
     1201 ggaaaacctg ctttgcccag cattcccgga acaacgataa cggaataaac catggccaga
     1261 aaggtcgtta agccagccag tacttcctga cgaacggtac tgccacgagc ggtaatttta
     1321 aaataattat ccagcgcgcc ctgtgacttt ggtgcgtccg gtgtttgtag gatgtctcca
     1381 gacataatga tgtcctcacg aggatgttaa agttttatta ttagttgcgt tcgtacacca
     1441 gacgaccatc aacgtaggtg cggtagatcg aacggtcatc gcccaacgtc atcatcacga
     1501 acaatttgtc gactaaagaa acagagttgt catagcgcaa ctgctgtagc ggagtggcgg
     1561 tgggttccat caccacgaaa tcagcctctt tgccaggtaa aaagttgcca atcaaatcgt
     1621 caaggcccag agatttcgct ccgccgagcg tggccaggta aaatgcttca tatgccgaga
     1681 ggcgatagcc ttgtagttgc aacactttgt aggcttcgtt tagcgtttgc agcatgttga
     1741 aagttgttcc ggcaccgata tccgttccca tgcccacttt gactttcttc tgccatgctt
     1801 ttttcaagtt gaataagccg ctgccgaggt aaaggttgga ggtcggacag aaagcaatgc
     1861 tggatttggt ttcgctgaga cgatcccact ctttttcttc gagatggacg cagtgagcaa
     1921 agacacagtt tttacctgtc aggccatact ggtgataaac atccagatag ccatcatgtt
     1981 caggataaag ttctttcacc caggcaattt catctttgtt ttcacagaga tgggtatgta
     2041 cccacgtatc cggatattct tctttcaggc gtttcgccat cgccatctgt tcaggagatg
     2101 aggtaggggc gaagcgtggc gtaatcgcat acaacagacg accatttttg tgccagcgct
     2161 cgatcagttc tttgctttgg tgatagctgc tttcggcagt gtcgagcaga taatccggtg
     2221 cgttgcggtc catcatcacc ttaccggcaa tcatacgcat attgatatgg ctggcggctt
     2281 caaacagcgc atcaacggat tgcggatgaa cagtgccaaa caccagcgcc gtggtggttc
     2341 cgttacgtaa aagctgcttg atgaagaacg ccgacatttc gcgggcgtac tctaaatcct
     2401 cataacgacg ttcagtaggg aaggtgtgtt tattcaacca ctccagcaat tgttcaccat
     2461 aggcccccac catttcactt tgcggataat ggatatgtgt atcgacaaag cccggtacta
     2521 tcagtttgcc gcgatagtcg cgcacgcgaa tagtgtcagg aatttgatgc tttccgtctt
     2581 cccattcacc aaaccattcc acttttccct gtttaatgag taataaacca tcctcaataa
     2641 accgcagcgc agaggcaatc tcctctgggt tatcgactgt acgggtgaca tcaataaaac
     2701 tgcctcgtac cgcttttaac gtgtgttctc ctgacatcat tgactccttt actgttaatc
     2761 catctcttcc tctgaggtaa taccaggcag aacgttttcc tgtcggtagc caccagggag
     2821 gataatattt aataaaatcg cagttaaccc gccagcacaa atagggtttt caactagtac
     2881 ataaatagag gctggcaata ttttaaaaat ttcgggatcg taggagacgc caagtcctaa
     2941 acctaaagaa gtggcgacaa taagtgtttc tcggcgcttt aaaccgttgg tgatgatgat
     3001 gcgaatccca gcgatagcaa tcatggaaaa catcaacgtc attgcgcctc ccagaactgc
     3061 cgagggtatg gttgtgaaga agccgccaat catcggaaac aagccgagga taaccagcat
     3121 taccgcgatg gttcgcccga cataacgtga agcgacgcca gtcatctgaa taaccccatt
     3181 attttgcgca aacgtggtta agggtaatga accgacagcg gaggcgataa cagaaaccag
     3241 accatctgcc agcacgccgc ctttcagtcg ggactgatac tcttccccct gaatagggcg
     3301 gcgagaaacc attgccgtgg cggtgatatc gccgacagct tccagcacgc taagcaaata
     3361 aatcgtgcca accaccagga actgatggaa gctgaaacta aacccgtatt tgaatggatg
     3421 cgggatggtg attaacggca aattgcgcat actgctgaaa tccaccatac ccaggcataa
     3481 cgacgcgata taaccgacgc atagcccaat ggcgatccct cccatgcgta gcaacggact
     3541 gcgacagcag ttaaagccga tcaccacaat caaaaccaat aaaccaacgc cgagatgttc
     3601 gtaattgccg aacgtaccgc tgcttttggc tgcaaatcct ccaccaaaat cgataatgcc
     3661 gactttaatc aggcttaagc cgatcatcag taccacaata ccgctgacag taggcgtaat
     3721 aacccgacgt aaatagggca ggataaaaga agagccgaca accagaaatg cgccaacgaa
     3781 ggagacgccg aggagcgacg acatgattaa ctcttcgtga aaaccgtcgc ttttcatgct
     3841 gctgcccagc gcaatcatga ccgtaacaaa tgaaaaattg actgactgaa ttgagagaag
     3901 accagaaccg acgatgccgt agcggtttac ttgtaaccag gtaccaatac cagaggcgat
     3961 catcgccata gaaaccagat aagcggttgt ttcagcggaa agctgtaagg ccgcacccac
     4021 gattaacgcg ggggttacca tcggaacgaa aattgccaac agatgggtaa tggcaccaac
     4081 gagagcctga tgaaagggag ggcgatcctc cagttcaaat ataaggtcag aacctgcatg
     4141 gtttatatca gacatccctt cactcctttt aaagtaaatt tgatcatctg aatcagtaaa
     4201 ataaattccc acgaatttgg ggcgcaggac gccttgagaa cggcttatct cattttcgca
     4261 gtcactattt agtcatcgtc caaacagaga aacaggccgc cgaagcggcc atttattatt
     4321 tatatttttt ccagcgcggt gagtattttc tccggtgtga aatgccattc gcgtaaccag
     4381 atgccgcatg catcgtgaat tgcggtagca atcgccggag ctgcgccatt tacaccgatt
     4441 tccgagatcg atttggcccc gaacgggccg actttatcgt cgctcggcac cagcacagcg
     4501 cggaaatcac gcggaatgtc accaattttc ggtgcaccgt aactgcgtaa atcacgcgtt
     4561 aacgggtgac cttcggcgtc ataaatgatc tcttcgctca tactgtggcc gatagcacgc
     4621 agggtggccc cgtagatttg tcccaacgct aactctggat tgaccggtgt accgcagtcc
     4681 agcaaggcgt agaatttatc caggcggatt tcacccgtac gcgtgttgac ggcaacttca
     4741 gcgaagtttg cgccatacgg gaaagcgaaa tcaggcgtga tataactgcc agttcccacc
     4801 agtgaaccaa agccggtgcc ggtttcgcct ttatgggcaa tatccccgaa actaacttcg
     4861 cctttcttgc cgcgcacgac gcccggcgtt gctagttgaa catctgccac tggctcaccc
     4921 aacatttgcg cgccgtggaa cagtattttc tcccgcagat tttctgctgc caaacgcgcc
     4981 gcgttacccg agaagcaagt accggacgag gcatatgcgc ctttatcaaa caacgcatga
     5041 tcggtatcac cggagataac atgcacgtcc tgcggtgggc agtgcagcac ttctgctgcc
     5101 agtttcgtca ccacggtatc cagaccagta ccaatatccg caccgccaga atgaacgata
     5161 aaggtaccgt ctgattccag tttgatcata cagttagcct gatcgatatc cgggataccc
     5221 gatttctgca tgataatggc gacgccgcga ccgatgtgcc agtcaccgtt ttgtggtttt
     5281 ggtgaagacc attggatcat ctcgcgaccc tgacgcagga tctcttccag tgcgcagctg
     5341 gcggcggaag gaacggaggt cggcgcttta ccttcaccga ttgcaccgag aattttcagc
     5401 tcttgccctt cgtgtacccg gttacgttcg ataatttcca gttggtcgat ctgtaactgt
     5461 tcagccagtt ccgctaatgc catggtgata gcgaagttac ctttcggtgc gccataaccc
     5521 tgataagcac catttgggca aatgttgctg tagtaggtgg tgacctggaa atcgacgtta
     5581 tcgcacggat ataacggcag cgacagcgcc ggtccgttac acggtacggt gagtgagtgg
     5641 ttgccgtaag ggccagtgtt ggcgcggaaa tccatcttca ctgccgtcag gcgaccatct
     5701 tttttcgctc ccagtttgac ggtgactttc gcgacgtgac gagaggtgtt agcaataaac
     5761 tcttcttcac gggtgtagcg gaacagtacc ggacgcccgg tcacgcaggt tgcccaggcg
     5821 cacacttctt ccagcaggat gtcctgttta gacccaaaac cgccgccaac tcgctcttta
     5881 atgacatgaa ctttatgttg tttcatgccc acgaggcgcg cgacctggcg gcgtaagtgc
     5941 catggtacct gggtggaggc gtggataacc agacgatcgc cgtccatacg ggtaaagcag
     6001 atatgtgttt cagtcgggca ctgctgcgcc tgcgttgagt tataggttcg ctcaatgatc
     6061 acatcggcat cggcaaagcc tttgtccata tcgccaatat gaccatgaat actggcggcg
     6121 atatttttgc gagggcgaga accgatcggg aagttgatga tcatatgctc gccgcgctgg
     6181 gctgcatggc tgttatcgtc ttccagagta tctggcgcac cagcaacata caccaccggt
     6241 tcatcgtgca cgacaggcgc atcttccgcc attgcttcgt cgatcgacat tacaggttta
     6301 agcacttcat attcaacgtc gatgagcttc agtgcttcga gcgcaatttc ttcactttct
     6361 gcgacgaccg cagcaacgcg atcgccgacg tgacgcattt tcttgccgaa catacggcgg
     6421 tcaagcggtg acggttccgg tgcgctctga ccgcccgggg tatagtagat atccgggcaa
     6481 ttcaggtgag taataacgtg aacaacgccc ggtaaggctt cagctttgct gacatccaga
     6541 tgagtaatca gtgcgtgagc gtgtgggcta cgtaacattt taatgacgca ggcgtcagcc
     6601 gttacgcggt cttcaacata gcagggtttc gcctgcacca ttttcgcggc atcagtttta
     6661 ggataatgct tgccaatgac ttcaaggtcg tcacggaaag tcggagcgat atcaatggtg
     6721 gcctgcggat tatttttacg tgcaaccgcc agttcaatga cctgatagta ttgctgccag
     6781 ccagcatcgc ggctaaacag acctgagagt gcatcgtcga tctcttcgcg ggtaggtgcg
     6841 tcgatgcgat cgagaagatc ggtgataatc agagctgcag ctggatcgtt ataaccagac
     6901 tgcaccacgc caacatcaac catggcctgt tgaaccagac ttaactcatt ccatttgccc
     6961 agagattctg cagtacgaat atctgccttc tctaactgtg cggcgataag caaggacgcg
     7021 ttaacgatat taccgttaaa gataattgcg tcagaaccgg caaacccaaa gccatcatca
     7081 ctgttgcgta cagagtgcat tcccatgtta aacaacagct tttgcacgtt ttcgcctgga
     7141 ttaacggtta gctcctgagg cgcgccattt aaagtaaagt ggatgatcat acggcttcct
     7201 ccccagcctg ttggcagtcg gcatacaggt cggctaccag aactcccgtg atatagcgtt
     7261 tataggccac gctgccccgc aaatcttcct gcgggaaaat ggcgttggcg acagcctgtt
     7321 caagtgcatt gccttccaga ttttgttttt cgacatcgtg cagacgcagg gctttactgg
     7381 cgacgccatc cagcgcaatt cgcataccgt cgtggtctgt cattgcaacg gctgccgtca
     7441 cgacggttaa acctgcctga gaacggctaa ttttgcgggt cgcacaggtg cgatacggat
     7501 ctttaatgat aatttcggtt agcaggcgat cgcatgggca ggccaggtag tcctcgattg
     7561 acagcgtttc gccgttgcca aaaaccagtt cagcatccag tgccagcagg acgggaagca
     7621 gcaccgactc ttcctgccgg gcggcgattt caccaccaat ggtcgactga ttacgaacat
     7681 ggcgtgagta aacaaaaccg agggcttcac gcagtgctgc aggaataaat cgcgcatcac
     7741 gcagtggctg caagcgagac attgcaccaa tccgcagtgc accgttatcc cagtcaaccc
     7801 agtccagttc cagatcctgt aaggaaatgg caatcttttt atcggtacgg gttggtgtag
     7861 cgttgagttt gctgcccccg gcgaaccaga cggcttcatc cttgtagcgg cgcttcagtt
     7921 ccagcgcctg ttcgacagag tcgggcctga aaaattgttc aatcatcttc tttccttatt
     7981 taaccctctt ccctgcggga agagggtatt tgtcatttat gccagcgcat ccatccgacg
     8041 ccacatactg gcagcggctt ttctggcttg cgcataaatg gaatcgcaat cgaagttaaa
     8101 ctgacggtct tcatagacca tcacgccatt gaccatcacg ctatgaacgc tgcctgagcc
     8161 cataccgaaa gcgatatgcc cggcgatatt gtctgcaagc agcggtgtcg gcgagttgta
     8221 atcgcaaatg gttaaatcgg ctttgtaacc ggcttccaga aggccaaatt tcgcgccaaa
     8281 gttgcggctc atcagttcgt tgccgttagc cagtgctttg gcaaaactgt caggccacag
     8341 cggaccaccc gcatcgcgat gtttaaagaa ggcaaatttc atctcttcaa acatgtccga
     8401 accaatgccg tcagttccca acgccagatt gcggatgtcg ctaaggtgat ggttgtagcc
     8461 gacatggttg ttcatgtttg aacgggcgtt atgcaccagg aacgcatcgc gctgattgag
     8521 tagggcgatg tcatctttcg acaagtacag cccatgagcg accagcgttt tgctgtcgat
     8581 gagatcgaat tgcgccagtc gtgccagcag gtctttgccg taccagtggt gactgtagga
     8641 aacgtcgtaa aggtcttccg cagcgtgaat atgcaaacca cgacctgtgg ctttcacggc
     8701 ttcacgcagc atctccagac cggcatccgg cacggtaaac ggcgcgtgag cgccgatatg
     8761 tgcttccacc agatacggct cggttgctgc tttcttcgcc tcatcaatct gacgggcgaa
     8821 gcggatgttt tcttctacac cttcctgcaa ctctttgatg ccgttgttac ggtcagtagt
     8881 ttcaaaacag gtcatcgcgc gcaggccaac ttttaaaaat gcgttgcgca atgtggagag
     8941 cgacccgccg atatacgccg gagaggcatg gtgatcgata accgatgtac atccgctctt
     9001 aatcgcttcc agggaacaaa tcagtccgct gtaatagagc gactcttcat caagggcgcg
     9061 atcgagtcgc caccagagat ttttcagcgt tgagatgaaa tccgggcagg gggcgatgtt
     9121 tgccataatt ccgcgggaca gtcccgagta aaaatggttg tgcgagcaga caattcccgg
     9181 catcacaatc cgaccatgca tctctttgta gctggcatcg gggtagcgtt gcgtcagggc
     9241 atcgccgata gcgacaatca catcattttc gatggcgata tcaacgcctt cctgcacttt
     9301 cgccgggtgt aactgcactg cggtgacatt cttcagaatc aacatgatta cacctccacg
     9361 cggcccagca gataatgatg atgctggtgg acatggctaa taatgcggca catatcgttc
     9421 agctccggtg gtacgttgtt gaactgacct tcgctgtcga tgtttaacac ccagctttgg
     9481 ttattcagac gtactcgtac ccggcaatct tccacaagga agccaggatt gctgctgtta
     9541 tcaaagtctt gcgacaggct gaagacggtg attttgtctt tgtacggttt accgttccac
     9601 gggcagaact gagcgcagtt gccgcattcg ttacagtaag cgtcgaggtg cagcgtctgg
     9661 aaacggttct ggaagcctgg gaccgcaatg gatacgttgg cgcggttcgg gcagacatcc
     9721 acacacttgc tgcaaacgta gttacattca aggcagcgtg cggcttcctg ggcgacaaac
     9781 gcgtcacgat cgtcgctatc caccagggtg atagagatat cgcctttgcg ttgatagatt
     9841 tccgccggat tgacgttgtt ccagtattta tcgttctggt gggaacggat gttttcccga
     9901 ctaaggatga catcggtcgc ccgacgcgcg gttccgacag cagcgacaat ggaggatggt
     9961 ccgcgctgta cgtcgccgat cataaagacg tcagtcagac gagtttcgcc attatggtcg
    10021 acgtctggcc agccgttttt gtccagcggc acgcccatcg cattcagggc ttcagtatcc
    10081 tgctgttcac caatggcggt gatcaggctg tctacatgca gtgttactgt ttcgttggtt
    10141 tcaaccggac ggcgacgacc tttctcatca ggttcgccaa gcgacataac gcgcaaggtt
    10201 aaggtgccat cagcatcgaa acgttccgga ttattcagga aacggaactc cacgccgtcg
    10261 tgcaacgctt cttcatactc ttcgcgccat gctggcatct cttgcagtga acgacggtaa
    10321 acgaccgttg ctttttctac gcctggaacg cgtaacgccg cacgagcgca gtccattgcc
    10381 gtgttacccg ccccgacaac gaccacatgt ttgcccagct tgagcgctgt gcccttgttg
    10441 tattcacgca ggaaggggag tgatttccag acattttggt tgtcgcccgc cagtttcaca
    10501 ccgctatttt tatcagtgcc ggtggcaatc agaacatagt ggaagccctg attttttaac
    10561 tgttcaacgg tcaaatcggg tgagcagcca tactcaaatt tcacgccgtg agcggcaaca
    10621 aaatcgatat cgtgctgaat taactctgca ggaatacgga actgaggaat gatatttttc
    10681 accacgccgc ccgcattggc ttcgcgttca aacagcgtaa ccggatggcc cgctctggca
    10741 aggaagtaac ctgctgccag acccgccgga cctgcaccaa tcacggcaac cggatggcgt
    10801 gaaccagaac cggctggttt gtgccagcgt tgcttatatt catcccaacc tttttccagc
    10861 gcgacttttt tcagttcgcg gatattcagc gcactgtcgt aatccaggcg ggtacagttg
    10921 tattggcact ggtgatcgca aatatgaccg gtaatggcgg gcagggcgtt gcgttggtag
    10981 atgagttcca gtgcgtcggc atagcggtgt tcgccaagca gacggatgta ttccggaata
    11041 tcctgtttaa tggcgcaagc agtaacgcac ggtgcaacgt agcagtcggt cagcggcagg
    11101 tcttctgcca cttcaatacg ctcttctggc ttccagtgtt tctgagtgta ttccatggtt
    11161 aacgcgtctg ctgccagttt gttcaggcgt tcgacgtcaa catggtcaag cccccaggcg
    11221 tcggagcctt ccagctcgcg catgcagaca cttaagcgca gatagccgcc aggtttcagc
    11281 aggtcggttg ccatagtaat agggcgaata ccagtatcaa aaatatcgcg gatagtcagc
    11341 tgactggcac caccggaata agaaatgggc agtttgccgt caaaggcgcg agagagaact
    11401 gctgcaacat tgatggagag cgggaacagc gcacggcctg acatatacat ctcttcacca
    11461 ggcagtgcgc ctttattgtt gatggtgccg agagtgttag tcagttttac gccaaagccg
    11521 agtgattttt cttttgccag taccatcagg cgttccagca tttccaacgc ttgcgtcagc
    11581 ttgaggtcgt gatcaaatga ctcttctttt aagcctatgt aaccgaaacc gcagacatcg
    11641 aggatctcac gaacacgcgc gtaccccagt aaggtcgggt tgagtttcac aaaggtattg
    11701 agcccttttt cttccagcat gtagcggcaa atggcttcga tttcatgcgg aggacagccg
    11761 tgcatggtgg agagggtaac gccttgtacc atgctggtgg ggatgcgtgc cggtaaggcc
    11821 tgcaggcatt cgcgtttttc ctgcaatccg tggcgggcta aaaatgcgtc atcctggagt
    11881 aatttattca gcgtatcgcg atattgagcg aatttcggat ggtcagatgc atccatcata
    11941 ttgtcgatga actgttgcat cggcggttgc ttaatacctt cgaggttata accgacgctc
    12001 atattaaaga tgaacgattt accggaatca gaaggctgga acatcgcttc gagaaggtgc
    12061 agggcaaacc aggctttgag gtattcatcc caggctttaa gcagggtaaa ttcggtagac
    12121 cattcggtgt taaagcactc gtcttcggca tcgatacagg gcttttccag ctccagacgg
    12181 tcaagaattt ggacggtttt tagttcgatg aagcgcccgc cagtcagcca ggaagtgaca
    12241 atattttgcg cgagctgcgt gtgcgggcca gcggcagggc cgacgggagt ggcacaggtt
    12301 tcaccgaaga cgctaacagt tttacctttt acgggtgagt aaaactgttg ctcgggaata
    12361 ccaaagattg agcgttgttg ttggtattca tcaaatatgc gcgtcaaaag ttcctcaaac
    12421 ggaatgggac gcataatatc ccccataacc ctctccttag cttatacaga tatcattgag
    12481 tgaccggtgt tgagaaatcc ggtggtttag ggggatagag caacaatcgc accactcttg
    12541 ccaggtttgg cgggaaatat aaaaaaatgt gaggcggctc gaaacttagg agctttttgt
    12601 gaatgaactc acaggtgcca aatgagaatt aaaatcactc ctgaaaatta ttattgccag
    12661 cccatttatc actttttctc ttttctcaaa atcagtgatt ccttatcatt atgataaaga
    12721 gtgctggagg gattaaatat ttgaacgcaa tcgtttaact acattttcta aattttagta
    12781 tacctttttt gcgctgtaat aaagtcgtcc ggagtatcaa tatctaaaat tatttctgca
    12841 ttttctattt cgacggaata atgatctccc ataaggagtg cctgacgcat attggtgata
    12901 ttgggacgct ggattgcctg catcaggcat ggctttgaca ctaaaatcgg atggccggga
    12961 atgccattat ggagtggcag tattgcgcca tcatttcgta acgaccagat ttttctaaaa
    13021 atatcgatgg tgagggtagg catgtcaccg tgggtgagaa aacaatattc tgtttttacc
    13081 gcgggtactg cggcctttac tgatgtgagt aaaccctgcg cataatctgg gttgtgaata
    13141 atagtgatat tgctctggtt cgcataacgt tcgtgcagtt cattaccacg atagccggtg
    13201 actaaaataa ttcggctaca aaactgcaac gcatttttga tacttgtatc aagaattgtt
    13261 ccctgttgcc agggtaacat cattttccat tgccccattc ttgatgataa tccggcagcg
    13321 gtaattatac agtcgatggc tgacatagtg gttccattat ttataggggc taataacgcc
    13381 tgataattgc gttacttcct gatggattaa gttggggata atttatccca gagaggtcat
    13441 aaagactcgt gaaaagtata gttgacccat cggcattatt cattgattta ggcgcgcaga
    13501 aacgtcccac tgtgatttct gttgttgggg ctggggggaa aaccagcttg cttttttggc
    13561 tggcagaact gctccaggct agcggcaggc gtgtgttaat cactacgacg acacatatgt
    13621 ttatgccaac gtctcactgg cccgtggttt tctgtcgtga tcccgccatg cttcctcatg
    13681 cgtctcttac atcccccatt tcattttgtt ttcacagctg gaaagcgaac cagggaaagg
    13741 tgcagggatt tacgccagaa gcgattgatg cactggtgca acgaccagag tgtgacgtaa
    13801 ttctcattga ggcagatggc tcgcgtggaa tgccgttaaa agcgcctgat gagcacgaac
    13861 cttgcatacc taaaagcagt tgctgcgtga ttgctgtgat gggaggacat attttgggtg
    13921 caaaagtgag cacagaaaat gtccatcgct ggtcgcagtt tgctgatatt actgggttaa
    13981 cacctgatgc acccttgcaa ctgagcgatc tcgttgcgct ggttcgccac cctcagggcg
    14041 cgtttaaaaa cgtaccgcaa ggttgtcggc gagtctggtt cattaaccgt ttttctcaat
    14101 gtgagaatgc gattgcgcaa agcgagctcc ttcaaccgct gcaacaacac aacgtagagg
    14161 caatctggct gggcgatata caagaacatc ctgcaatcgc gcgcagattt gtgaattagc
    14221 ggcccgggta ttccgggtta ctgaaaggat atccattgag gttggtatga atattttcac
    14281 agaggctgca aaactcgaag agcaaaattg tccgtttgcg atggcgcaaa ttgtagatag
    14341 ccgaggctct actccccgcc attctgcaca aatgttagtg cgcgccgatg gttctatcgt
    14401 cggtacaatt ggtggaggaa tggttgagcg gaaggtgatt gaagagtcgc ttcaggcatt
    14461 gcaggaacgt aagccgcgat tattccatgg acgtatggct cgtaacggtg cggatgctgt
    14521 cgggtcagat tgtggaggtg caatgtcagt atttatcagc gtccatggta tgcgtccacg
    14581 tctggtgttg attggcgcgg ggcatgtcaa ccgggcgata gcccagagtg cggcgctatt
    14641 aggatttgat attgccgttg ccgatattta tcgcgaaagc cttaatcctg aattattccc
    14701 accatcaacc acgcttctcc atgctgagtc gtttggtgcg gcagtggaag cactggatat
    14761 tcgccctgat aattttgtcc tgattgccac gaataatcag gatcgtgaag ccctcgacaa
    14821 actcattgaa cagcccattg catggttggg gttgctggca agtcgtcgca aggttcagct
    14881 tttcctgcgt caattgcgtg agaaaggcgt ggctgaagaa catattgccc gtttacatgc
    14941 gcctgttggt tacaacatag gtgcggaaac gccgcaggag atcgccatca gcgtgctggc
    15001 agaaatatta caggtgaaaa ataacgcgcc gggtgggctg atgatgaaac cttctcatcc
    15061 ttccggacac cagctggtgg tgattcgcgg tgcgggggat atcgccagtg gtgtggcgct
    15121 acgtctgtat catgcgggtt ttaaagtgat catgctggaa gtggaaaaac cgacagtgat
    15181 tcgttgtacc gtggcgtttg cccaggccgt gttcgatggc gaaatgacgg tcgaaggcgt
    15241 cactgcccgc ctggcaacca gctctgcgga agcgatgaaa cttaccgaac gcggattcat
    15301 ccctgtgatg gtagatcccg cctgttcatt gcttgatgaa ctgaaaccgc tttgcgtggt
    15361 ggacgctatt ctggcgaaac agaatttggg gacgcgggca gatatggcac cagtaacaat
    15421 cgcgcttggg ccgggcttta ttgcagggaa ggattgtcat gcggtaattg aaacaaatcg
    15481 cgggcactgg ctcggtcagg tgatttactc tggttgtgcg caggagaata ccggtgttcc
    15541 tggcaatatt atggggcata ccacccgacg ggtgatccgt gctcctgctg caggcattat
    15601 gcgatccaac gtgaaattag gcgatctggt gaaagagggc gatgtgattg cctggattgg
    15661 tgagcatgaa attaaagcac cgttgacggg gatggtgcgt ggcttgttga acgacggact
    15721 agcagtggtc ggtggtttta aaattggtga tatcgatcct cgtggtgaaa cggctgattt
    15781 caccagcgtt tctgataaag cccgggcgat tggcggcggc gtacttgagg cgttaatgat
    15841 gttgatgcat cagggtgtta aagcgacgaa agaagtgctg gaagtggctt aaatgaaaat
    15901 ggccggatgg tatttaccca cccggccttt aaattactga caaatcaccg ttccggtttt
    15961 gccttcaatc ccttctttcg ctttgctcaa tacggtaatc agtgcttcac gacctgcgcg
    16021 agagcgagca aacgaggcgg cagcttccac tttaggcagc atagaacctt tcgcaaaatg
    16081 accttcttcg ataaagcgtt cagcatcact taatgacagg cgatcgagcc actgttcatt
    16141 ctctttaccg aagttaatgg ccactttttc taccgcagtc agaatgatca gcatatcggc
    16201 atcaatcatt tctgctaaac gggcgctggc ccagtcttta tcgataaccg cactggcacc
    16261 acgcaggtgg ttaccttcac gaataacagg gataccacca ccgccaacgg tgatcaccac
    16321 ctggcctgca tctaccaacg ctttaaccgt ttctttttca atgatatcaa ccggttttgg
    16381 cgaggcaaca acacggcgat agccgcgacc agcatcttct ttcagagtgt aaccctgttt
    16441 tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg atcggcttgg tcgggttgag
    16501 gaatgccgga tcgttagcgt cgacttcgac ctgggtaacc agcgttgcta caggtttatt
    16561 aatgccacga gaaagcagtt cttctcttaa ggcattttgt agatcgtaac caatataacc
    16621 ctggcttaag gcaacacaga cagacatcgg cagcattggc gagtgcgctt cagtttttgc
    16681 ggcagcttca aaggcctggt taatcatgcc cacctgcggc ccgttaccat gagtgacgat
    16741 gacctcatgt ccctgggcaa ttaaatcaac aattgcctga gaagtaattt ttaccgcttt
    16801 catttgtccg gccaggtcat cgcccagcgc attcccgccc agggcgagaa caattttctt
    16861 actcattttt cctcactcat cattcttgat tgtgtataga tccctggcag tggttagagc
    16921 acgggaggga caaacggttt gcgtcgcagg aatcgacctc gcccggcttt gcctgtaaag
    16981 gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc tgacaatcgc gccctgacag
    17041 gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga gatggcgatg ctgaatttgt
    17101 tggctctgac gtgggtcgat aatcaccacg tcgccatcgg aaccgggcgc taataatcct
    17161 ttttgcggcc acaggccaaa cagcctggcg ggcattgcgc tggttaattc aacaaagcgt
    17221 tccggtgtta tacgtcccgt catcacgcca ctggaaaaca gtaactgcat gcggttttcc
    17281 acaccgggta agccatttgg gcagcgactg aaatcgcctt tagaaatttg caggcgttga
    17341 gccatcgaga aggtgcagtg atcggttgcc accacgtcaa tcgcaccatc gctgatgcca
    17401 caccacagtt tgtcctgctc gcgtacgtta cgcagcggtg ggctaagaat gaacttcatg
    17461 ccatcttctg tatcgtaact gcgttcgtcc aacaggagat attgtgggca ggtttcaacc
    17521 cagactggct ggtgattcgc acgggcaaga cgcagataat ccagacctaa gccgttagac
    17581 aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg ccaggttaat catgcgggcg
    17641 atggcttccg cttcgcattc cagagggcga ctcaaggcgt gatagcgcgg cgcggttaac
    17701 cctgcggcaa taaactccgc ccgcttgctg gcgatagccg catcattttc cgggtgcacg
    17761 gtggtcagcg cgccggattc atgcaagcgg cgtaatgcct gcaacacttc atcatcgttg
    17821 agtttgtatt gataggttaa atagagttta aaactgctca gcccttcctc gaccatcatc
    17881 gggatttcgt cgaggatggc gtgattaatg tgctggatca caccgtgaaa gctgtagtcg
    17941 atgaccgcct tatgggcggc ataaccacga taaacctcca gttgatggcg taaccggcag
    18001 ccattcgggc caaatcccat atggtcaata atggttgttg taccgccaca cgcagctgcg
    18061 cgggtaccgg taaaaaaatc atcacaactg cgcgcgatgc cgacatcaat attgaaatgc
    18121 gtatggacat ccacgccgcc agggaaaacg taacagccag tggcatcaat ttcttcatac
    18181 gggagctgtg gcgaaatatt gttgcccaac tggcgaacaa tcccgctttc aatcagcaaa
    18241 tcctgtttgg cttgtccatc tgcgttaacg acagtgccgt ttttgatcaa tacgcgcata
    18301 gcaaactcca aaaaccctcc ggcgaaccgg aggggaataa gtgaagagta attattcggt
    18361 tgccagccag cttaacggga ttgcagcgta catcgcggca caggtcacca ggtgagattt
    18421 ccaggttttt tcgttaggtg catgcgcttc aggttcttta cccgggccaa agccgatcac
    18481 cggaatgccg tgacggccca tgatagatac gccgttagtt gagaaggtcc acttatcaac
    18541 aaccggcgct ttgccaaaca gaccttcgta ggcattcacc agtgctttaa cggtgaagtg
    18601 atcttcttcc actttccagg tcgggaagta gcattcggtt gggtaaacca ggccagtcca
    18661 ggacggacgg tcgtagttgt acatagaaac aaccgcgtta gctttctgta ctgcaggcag
    18721 ggcgcggatt tcgtccagcg cgccttccca ggtttcgccc caggtcagac ggcggtcaat
    18781 agagacggcg cagctatctg ctacagcgca acggcttggg gatgtgaaga agatttcaga
    18841 aacggtgagg gtgcctttgc cgaggaattc gtcataaccc agacgttggg agagttcttg
    18901 taattcgcca agaatcggac ccattttgaa aatggcgttg tcaccgcgtt ctggtgcaga
    18961 accgtggcag ctaacaccct gaacatcaat acgaatttcc atacgaccgc gctgaccacg
    19021 gtatacctgg cagtcggttg gttcggtact gaccacaaat tccgggcgaa tgccggattg
    19081 ttcaataatg tactgccagc acaggccgtc gcagtcttct tcctgcacag taccggtaac
    19141 cagcagggta tattcatctt ccagaccgag gtctttaatg attttaccgg cataaaccat
    19201 agatgccatg ccgccttcct ggtcggaagt accgcgacca ccgatcagct catcagtttc
    19261 catgccttcg tacggatcga agtcccagtt tttgatgttg ccaatgccga cggtatcgat
    19321 atgagcgtcc attgccacca gacgcgggcc gtggccgata taaccgagaa cgttgcccat
    19381 cgggtcgatt tcaactttat cgaagccgac tttttccatc tcttctttaa tacgatgtac
    19441 tacgcgtttt tcgtcgcagc tttcactggg aatagcaacc atgtcgcgca ggaagcgagt
    19501 catgtccgcc tggtaatctt ttgctttttc aagaatcagt ttgaatggaa tattcttagc
    19561 cattattttt ctccagattt tttactcccc gttttgcagg gagcagttcc aatagggtga
    19621 ttaaggtgct acagcgtgtt tgccttccca gacaacttcg cggtagtgct tcacgtcggt
    19681 gtcgccttca gtgctgataa ccagcactac ggcatctttg ttcagcgcca gtttttccat
    19741 caggctttga cgttgcgggt gataatgaac cgctgcgaga acgcccaaac cgacagcgcc
    19801 ggattcaccg gagatgatgc gcgggtcatt gccgtacgga ttacccagca cacgcatacc
    19861 taatgcggca acgctgtcct ggcaggagat gaattgggtg gcgcagttac gtaggatttc
    19921 ccagcccagc gggttaggtt cgccgcaggc caggcccgcc atgatggtgg ccatatcacc
    19981 gccaacgttg acgatgtcgc ctttgacgcc ggagcgataa atacagtcag ctttgtcagg
    20041 ttcaacaata atgctgtgca gattttgcgg gctgtagacg tcgaccagat aacccagcac
    20101 accaccggcc attgcaccga caccggcttg cagcagaacg tgtgtcgggg ttacgcccat
    20161 ttcacgcatt tgctcgacgg cttcatctgc cagtgttgcg tagccttgca tgatccaggt
    20221 tgggattttg gtgtaacctt cccatgctgt gtcctgtacc acttcccagc cgtgctgctg
    20281 cgcgtgttgc atggtcaggc gaacggtatc gtcatagttc atatccgtga cgatgcactc
    20341 ggcaccgagg ttcagaatgg cgtcaacgcg ttcctgagca gaacctttcg gcatgtaaat
    20401 caccgcattc tgtccgagtt gctgtgctgc ccacgccaca ccgcgcccgt ggttgccgtc
    20461 ggtggtggtc gcgaaagtca ttttttcgcc gatggcattt ttcaggtgct caaatgacag
    20521 cgtttcgata tcaagatgat atttttcgca caataattga gcgatggcgt atgcaccgcc
    20581 gagcattttg aaggcgttca gaccgaatcg ttttgattcg tctttgacga gaattttctt
    20641 cacaccaaaa aggtttgcga gatcgtccag cgcacaaagc ggtgttgggc gataaccagc
    20701 aattttctgg tggaactggc gcgccagttt ggcctggctt tgcgaaaaga gcggtgatgt
    20761 ttcgccgttg aaaaatttgt tatcggcgat atcaatcttc aatgagaaaa cggacatatc
    20821 ctatcctttt ggcctctgga aatagagggc cgaccagctg atcggcccga ggggttattt
    20881 cacgcgttct tgcgcttctt tcagcagttg ctcgagcagt gcacctggtt tggcgtattt
    20941 acgggacagg atcatcgcgg cgatgatata aggcttccag ctggcttctt tgtaggttgc
    21001 gatacggtat ttttcgaata cgccttcagt cacttcacct tctttacagg atacgccgct
    21061 gatatcagct ggaaggcagt gcatgtacag ggcttcgcca tcacgggtca gttccatcat
    21121 ctcttcagta caatgccagt ctttgtgttg cgcgttctgt gccagacact gtttttccag
    21181 tgcttttaag ccttcgtggt cgttcgcacg cagcaattca gtacgctctt ccatcacttt
    21241 gtaaggtgcc catgacttcg gataaacgat gtctgcgtct ttgaaggctt cttccatgct
    21301 ggtgacctga cggaagctac caccggatgc tttagcattg tttttcgcca cttcaaccac
    21361 atccgggatc aggtcgtagc cttccggatg ggccagggtg acatccatac cgaagcgagt
    21421 catcagaccg atgatgcctt gtggtacaga gagcggtttg ccatagcttg gggagtaggc
    21481 ccaggtcatg gcgatttttt tacctttcag gttttccagt gaaccaaagt gttcacgtaa
    21541 ccatgccagg tcagccattg actgagtcgg gtggtcaata tcgcattgca ggttcactaa
    21601 agccggacgt tgcggcagca caccctgctt gtaaccgtca tcaagtgcag cgccaacttc
    21661 acgcatatag gcgttgcctg cgcccagata catatcgtca cgaataccga tggcatcggc
    21721 gcagaaggag atcatattgg cggtttcacg cacggtttcg ccgtgagcga tttgtgattt
    21781 gccttcgtcg agatcttgct gtgcaaggcc gagcaggtta agcgcggaag cataagagaa
    21841 gcgggtacgg gtggagttgt cgcggaatac ggaaatacct aatccactat taaagacttt
    21901 ggttgagatg ttttcagcac gcagtgcttt taatgctgcg gcaacgtcca gtacttgttt
    21961 cagttcatct ggcgtctgtt cccacgttaa caaaaaatct ttctcgtgaa ggtgagaggt
    22021 cagcgaattg atatccttaa tcagctcatt aacagttttc atcataaagt cccggtagtg
    22081 gaatgacagg caaagaggtt tatttcactg tgacttgcca aagtgattgg tgttttcaca
    22141 gctatacctg ggactaacaa taactgtgcc agaattatcc aagcggctgc aaaagactaa
    22201 tttgacgggt aatgtgtggg gttgatcaca aattgatgcc agcttctggt tgtttgtaaa
    22261 atacgaaaac gtttggcttt ttttatcgcg ttatttattt gctgctaagt atcagaaata
    22321 agataaaggg gcaggttatc cctcgtcagt ttgataaact caccgccgta attctcatat
    22381 tgatatagaa atgtgatatc tcttccataa tttcattaga aagtgataaa aatcttatgt
    22441 ttttatcaaa gccaatttcg cctgagttaa tatcatcatg atctatgaac catcaacgtc
    22501 ttcaatgatc gacttttaag taagtctgga aggtaaagct atggagcttg ctactacgca
    22561 gtcagtattg atgcaaattc aaccgacaat tcagcgtttt gccagaatgc ttgccagcgt
    22621 tttgcagctt gaggttgaga tcgttgatga aaacttgtgt cgcgtcgccg gaacgggcgc
    22681 gtatgggaag tttcttggtc gccagttgag cggcaactca cgcctgctcc gccacgtcct
    22741 ggaaacgaaa actgaaaaag ttgtgacaca gtctcgcttc gatccccttt gtgaaggttg
    22801 cgatagtaaa gaaaattgcc gcgaaaaagc atttctgggt acgcctgtca ttttacagga
    22861 tcgttgtgtt ggggtgataa gtttgattgc cgttacccac gagcaacaag agcatattag
    22921 tgataattta cgcgaatttt ctgattacgt tcgccatata tccaccattt ttgtttcgaa
    22981 acttctggag gatcagggac caggagataa catcagtaaa atattcgcga ccatgatcga
    23041 taatatggat cagggtgtat tagttgttga tgacgataat cgggttcagt ttgttaatca
    23101 gacagcctta aaaacactcg gtgttgtgca aaataatatt attgggaaac ctatccgttt
    23161 cagaccatta acatttgaga gtaattttac ccatggacat atgcagcata ttgtttcgtg
    23221 ggacgataaa agtgagttaa tcattggtca attgcataac attcagggcc gacaattatt
    23281 tttaatggcg tttcaccaat cgcataccag tttttctgta gcaaatgcac ctgatgaacc
    23341 gcatattgaa caattggttg gcgagtgccg tgttatgcgg caattaaaac gactcattag
    23401 ccgtattgca cccagcccat ccagcgttat ggtggttggt gaaagcggca cgggtaaaga
    23461 agtcgtcgcc cgtgcaatcc ataagttgag cggaagacgg aataaacctt ttattgctat
    23521 caactgtgcc gcgattccgg agcagcttct ggagagcgaa ctgttcggtt atgttaaagg
    23581 cgcatttact ggcgcttctg ccaacggtaa aacagggttg attcaggcgg cgaatacggg
    23641 cacgctgttt ctcgatgaaa taggtgatat gccattaatg ttgcaggcta aattactgcg
    23701 cgctattgag gcacgtgaaa ttctgccgat tggtgccagt agcccaatac aagtcgacat
    23761 tcgcatcatt tctgcaacta accagaattt ggcccagttc attgccgaag gtaaattccg
    23821 cgaagatctc ttctaccgac ttaatgttat cccgataact cttccaccgc tgcgtgaacg
    23881 ccaggaagat attgaactac tggtgcatta ctttttacat ctgcataccc gtcgtctggg
    23941 atcggtttat cctggcattg ctcctgatgt cgtcgaaata ttgcgtaagc atcgttggcc
    24001 cggaaacctg cgcgagttaa gcaatttgat ggaatatctg gttaacgttg ttccttcagg
    24061 tgaagttatc gatagcacgc tattgccgcc aaatctgctg aataatggca caacggaaca
    24121 aagtgatgta acagaggtca gcgaggcgca cctggcactc gatgatgcgg gcggcacggc
    24181 gctggaggag atggaaaagc aaatgatccg cgaggcgctt tcacgtcata acagcaagaa
    24241 gcaagttgct gatgaactgg gcatcggtat tgctacgctc tatcgcaaga ttaagaaata
    24301 tgagttgtta aacacataac ctcagtgaat ccggcctgaa ttcaggccgg atatcctttt
    24361 acttcgtttt ctcgcaatcc agaactgtat ttacaatcat ctgatacccc gtgcagcgac
    24421 aaagatttcc cgccagaccg cgacgaattt ccgtaatggt taatggcttc tcgcgcggct
    24481 ttgccagcat tgccgtggta gccataatca ggccaggcgt acaaaacccg cactgcaccg
    24541 cgccggattt cgcataagcc tgctgaacat gagaaagttt tccgcctttc gcttcacctt
    24601 ccagcgtgcg gatctctttt ccttcagccc aggcggcaag gtataagcaa ctgtctattg
    24661 ctgtgccgtc gaccagcacc gtacaggcac cacattcacc cacgcagcac ccttgtttga
    24721 cacttaacag tccttgatcg cggagtaatt ccgagagcgg catgcctggt gcggcgtgaa
    24781 gctgaaaagg catcccgtta atggtgcatt cgatggtaat tgtttcgctg tgattcattg
    24841 caattttccc cccgccgcgg cgacggcttc gctaatcact tttttggtca tcgtctggat
    24901 gagatgcaga cgaaactctt tactggcccg ccatgaagaa cgcggggcga catcttgcag
    24961 gacagattcg ctgatagctt ccagcgtttg caggtttaat ggcgcatttt gtgcagtctg
    25021 ttcggcatgt tggcagcgaa tcggcgttgg cgcggcaaca ccaaatgcca gacgtaattc
    25081 gctgaaattg ccgttatcca gtcggcaatg tgcggcgcag ccaatcgttg aaatatccat
    25141 tgcgtcgcgc atggcatatt taaaatgcgc gctgcccgcg tgttctttcg gctggggcgg
    25201 aaagtgaaag gcgacaagga tttcgtcatg ctcaagagac actttgcccg gtccggtgtg
    25261 aaagccatta atcgggacga aacgaacacc gcgcggggag tggatctcca gtttcgcgtc
    25321 ataaatcagc gttggcgtgg cagaatctgc gctggtggca ccgttgcaaa tatttccacc
    25381 gtaggtagcg acattacgga tctgtggtcc agcaatggac gaggccgcag cacataacgc
    25441 cgggagatga cgttgagtta caggatcttc aatgagctgg gtaaatgtcg ttgcagagcc
    25501 gatacgtagt gagccatctt ccgccagcgt aattccccgc agctccgcca gattgtgaat
    25561 atcaacaata tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc
    25621 accggcgagc aatttggcct gcgggttgtc agtcagcaag ttgatggcat cggcaagggt
    25681 tgctgcgcga tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca
    25741 gatggaactc ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg
    25801 tagccatctt caccgcgtta cgaatagcag cggcaacagg aattattggt ggctcaccca
    25861 gtgacttatg tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt
    25921 gtggcagatc cggcatggtg ggcattttgt aatccagcag gttggggtta cggaccacgc
    25981 cgcttttcgc atcaatgatc atctcttcaa atagcgccca gccaatgccc attcccattc
    26041 cgccgtgtac ctgaccttct gccagtagcg gattaagaat atggcccgaa tcatgaacgt
    26101 tgaggatgcg gttgatggtg actttgcaca gcgcaatatc gatcgtcaga tcaacaaagg
    26161 tacagccaaa cgctggtggg ttagtggtgg ttttgatgga gctttcggca gagagctgcc
    26221 cgccgcgttc agggtggtag aaggcatcca tcgccaaatc ttttaacgac attaacggct
    26281 cttccggtcg ttcaaccagc acgatatggc cttttatcaa ggtaagattc atcgctgact
    26341 gatgtagcat gacagcggcg tgagcgatga ttttctcctt taataacagt gccgcactgc
    26401 gcagcgcagg cgcggcaaca tagctctggc gtgaggcaaa tgcaccggga tcgaacggcg
    26461 taatgtcggt atcttgggtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt
    26521 ctgccaccat ttgcgagaag acagtgtcgg caccctgacc gatttctgtc gcgccgcttt
    26581 gcacgttgat ggttccatcc tgattcatca acaggcgcgc gcctgctatt tctacgccga
    26641 caggccaggt gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgtaaattgc
    26701 cttgctggtt ctggcactct gcacggcgtt tttcccattc aaagattttc cggccttttt
    26761 caagacactc cggcaaccct gcgctgtaaa tacgttttcc tgtgagcgga ttagcatctc
    26821 cttcgcgggc ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg
    26881 cgtcatcaag catagactca acggcaaata cgacttgtgg cgcgccataa ccacgcatcg
    26941 caccagccga ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag
    27001 gataaaggta agcgacttta ttccctcccg cagaagcgat ggagtgcccg tgagatgcat
    27061 aagcgccggt gttagacaga acatccagac tataaccttt caatgttccg tcgcggttca
    27121 cgcccatttg cccgtcaata gtgaaagcgt ggcgggtacg ggttgcgagg aaacattctt
    27181 cacgactaag ggaaactttc accggaatgc cgccaagctt gctggtcagg aatgccgcca
    27241 ttggctcttc cagtacatcc tgtttattac caaaaccgcc accgacaaac ggtttgatga
    27301 ctcgtacgca tgaccaggga atattcagcg cctgaccaac cacacggcga acaatgtgcg
    27361 ggatctgggt gctggaaacg atggtaattc gcgagtcatc ctccatccat gccagcgatg
    27421 tcacgctttc catgtgacaa tgttgaatga ccggcgtctg atagtgcccc tgtacctggt
    27481 agtcggcggc atcgattgtt tgttggacat tgcccgtcga catcgtgctt tgttttagta
    27541 aattgccgcc gttatggatt ggttcagcgt catcagccag cgccgcttct ggggtggtga
    27601 taacgggtaa ttcttgccac tcaatgctga ccaattgcgc cgctttttct gccgtgagtt
    27661 catcacgggc cacgacgatg gcaacggcgt ctccatggtg acgaacatgg cgagttagca
    27721 gtgcgcgatc ggcggtatcg cgcttgtttt cgtcaagtgt ccaggcatgc ccagctgtag
    27781 cgaatgggat atcaggtaca tcttcccagg taaaaatcgc cagtacgcct ggtaaacttc
    27841 tggcttgttc atcattaata cttacggcat aaccatgtgc gatagggcta cgtacatatt
    27901 tcgcgtaaca catgcccgcc ataacataat cgtcagtata tcgtgcccgc ccggtgacct
    27961 tagcaatggc atcgacgcgc atgcaagatt cacccgtagc ggttgcttcc cgcgcttcca
    28021 tagaaatccc ctcgatttga acatttggtt cttatggcaa agatttagtc taagcaggca
    28081 agatttacgc cagaaacgac gataagcgtt aggggaggaa ggaagtgtga tgacgttaaa
    28141 aaaacacaga tatgatacga aacaggactc tgaatagggg cagtgatacg tatcaatatg
    28201 agtggcgata aaatcaatgt gataaatcag gattattacg catcaggatg ggaaataggg
    28261 taaaaaagtc aggaaattcg atacctctgc agcgaatatt ttgctgatga tacttccctt
    28321 tgccaaatga aattaacctc tttctctatt accgcgtttt tcgagaagca agagattggg
    28381 tatccagagg ctggctgcta tgatatggcg tcttgttttt taagcgagga aagattttga
    28441 gtgcgggacg cctgaataaa aaatctctgg gtatcgtgat gttgttatcg gttggactgc
    28501 ttttggcggg ctgctcgggt agcaagtcat ccgatacagg aacgtattcc ggctccgttt
    28561 acaccgtgaa acggggggat acgctatatc gtatttcgcg caccacggga accagcgtaa
    28621 aagagctggc gcgactgaac ggcatttccc ccccttacac cattgaagtt ggtcagaaac
    28681 taaaactggg tggggcgaaa agtagcagta cacgtaaatc aaccgccaaa tcaacgacca
    28741 aaaccgcatc ggttacaccg tcatcagcag taccgaaatc ttcctggccg ccagtagggc
    28801 aacgttgttg gttatggcca acgacaggga aagttatcat gccgtattcg acagctgatg
    28861 gcggcaataa agggattgat atctcagctc cacggggtac acctatttac gccgcgggtg
    28921 caggaaaggt ggtgtatgtg ggcaaccagc tgcgtggcta cggtaatctc atcatgatta
    28981 aacacagtga agattacatt acggcttacg cccataatga cacgatgctg gtaaataatg
    29041 ggcaaagcgt gaaggctggg caaaaaatcg ccactatggg gagcactgat gcggcatcgg
    29101 ttcgcctgca tttccagatt cgttaccgtg ctacggcaat tgatccgctg cgttacttgc
    29161 cgcctcaggg cagcaagcca aaatgctgat ggcgaattaa tcagcagtca gcagcgcggc
    29221 acttgctaag gagagcgtaa ggtttataat gccttacgca tctcgaagcg ggcgtagttc
    29281 aatggtagaa cgagagcttc ccaagctcta tacgagggtt cgattccctt cgcccgctcc
    29341 aatttatctt cacctcatca acctgccatt tttgttgtat ctgattttct atattatctt
    29401 aattcaatcg cttcagactt ttcgcaatcg ccgtaatact tccccatttt tttgtaaata
    29461 aaagcgttta cgcaatgcaa tccatttgta acaacccagg taaaatacct tattttcaag
    29521 ttgttaaata agtggttatc tacgctttta acaaacgatt gatggaatct tttatgaaag
    29581 ggaaatctac attaactctt ctgctcgctg gtatattttc atgcggcaca tgccaggcga
    29641 caggggcaga agtgacgagt gaatctgtat ttaacattct taattccacg ggggcagcaa
    29701 ctgataaaag ttatttatcg ttaaatccag ataagtatcc taattatcgt cttctgattc
    29761 actcagccaa attaaaaaat gaaataaaga gccactatac caaagacgaa attcaaggat
    29821 tgttaacatt aacagaaaat acacgaaagt taacgttgac ggagaaaccc tggggaacat
    29881 ttattcttgc ttctactttc gaagatgaca aaacagcggc agaaacccac tatgatgctg
    29941 tctggctgag ggatagttta tggggctata tggcattagt atcagatcag ggaaatagtg
    30001 tagccgcaaa aaaggttctg ctcactctgt gggattatat gtctacgccc gaccagatta
    30061 agcggatgca ggacgtcatt agcaacccaa aacgactgga tggaatttcc gggcaaatga
    30121 atgctgtgca tatccgattt gacagcaact cccccgtgat ggcagatgtg caggaagaag
    30181 gcaagccgca gctgtggaac cataaacaaa atgatgcgtt aggtctttat cttgatctcc
    30241 ttatacaagc aatcgacact ggtactatta atgctgaaga ttggcaaaaa ggtgaccgat
    30301 taatatcgat tgcgctgttg gttgcttatc tggataaagc caatttctat gttatggaag
    30361 attctggagc gtgggaagaa gatgcccgat taaacacatc ttccgttgca ctggtgacct
    30421 caggactgga acgtttgtcg aacttactct cgaagaaaga ctctgttttt atctctgatt
    30481 tactacgcga agctaaagcc aatgaactgg atgaaccact ttcaacaacg aggttgaacc
    30541 acctcataga taaaggttat gaaagaataa cgttacagct tgatttaggg ggagaatccc
    30601 caggttatct ggaaaaagat aaacattaca gagaagccga tgcggcttta cttaatgtta
    30661 tttatcctgc aaatctttca aagataaata ccagaagaaa agagcaagtt ctaaaaatag
    30721 ttaaaaaact agcaggtccg tatggtataa aaagatacga gaaggataat tatcagtcgg
    30781 caaatttttg gttcaatgat attaaaactg ataccgatca aaattcacat gcgaaaagag
    30841 aaatgagctt cattccatca acagaagcag aatggttttt tgactcatgg tatgcaaaat
    30901 cagcagcaat tgtttataaa gaatcaagaa aagaagagta tttgaatgac tcagtgcaat
    30961 ttatgaatag atcattggcc caaataacag gcgagaatat gattggtgca aatggcagga
    31021 gtgtacctga aatggcactt cccgaaagct ataactatat ccacaagtcg ggaactttgc
    31081 atgaagcgcc aagccctatc attccgctaa attggtcgaa agcttcaatg actctcatgt
    31141 tgaaagagat gtccaacctt atcaatgatg agggaattaa atagttttca taatgaggcg
    31201 gtcctggatt aaaaggaccg ctcaaagaga aatcgtaagg gaggtaaaag tgatcaataa
    31261 attactgtta gcttacctga taggtctggt tgtgacatcg accttaatat ttatcttctc
    31321 ggaagaaaaa gtaacatatc ggttgtttgc tgcagtaatt acagggttga cctggccgct
    31381 tagcctcatt ccatccatca taagtctcat gattcgtaaa tccgactaac aagaaaatta
    31441 tatgaacgat taagaaaaat agaatcgaaa aagagaataa aatatatgca gggagtaaca
    31501 aaacactcgc agtgcgttaa tgtgtaacta acaaatatga tttttattga aaatagtcac
    31561 caaacaagtg ttataagaaa tacggggtat gtttaagtaa aacatatcaa cagttgcaat
    31621 tctgtatatg gacagaatga cttactactg taagtagctg atagctattc gctcataaca
    31681 aaatcattat tttataaata ggaatcatca ctttttaatc atgtttttat tgcaagttta
    31741 tggagaatga gaatatggaa taaggagatg aaacatgatt gaagaagggc tgttacttcc
    31801 aatgaatgtt ttgcttcaag gacagaagtt aaccctactg gaccctgaaa tgtggttctt
    31861 acggggaaat gaaaacaagg atgtaaccct tgttattacg atcggtaata accatcaaaa
    31921 attaatggta gtggaagata tattactgct cattgaccga agccaaattg aggtaactgc
    31981 agggaaagta atttatcatc cacttcgcat tgatatcctg agtaaattat tagcttttat
    32041 agatgaatca acggggagtg tggaaaggga acaccatgag ttgtttgctg atgccttacc
    32101 ctttgcatca gaagttgtgc tattcagtaa agttgcaagt gaagcctggt ttttagccac
    32161 ttatctgagc agcgataata ttaatgaaat tctgtttcag catctaagaa aaacagaatg
    32221 ctataaactg gttcgctatt tattatcgca atcgctcata cagacttcac tctatgatct
    32281 tggtgaactt tacggcgtat cgtattcaca ttttcgccgt ttatgtagtt atgcattggg
    32341 tggtaaagtt aaaacggaat tatgtggctg gagagttgcc agagccgtgt tggaaatcat
    32401 tgaaggaaac agtgatatga caactattgc gcataaatat ggctactcat cctcatcaca
    32461 cttttcagcc gaagtaaaaa gccggttagg aaaaacaccg agagaactat gtaaaaagtt
    32521 atgaaaataa aattacgtat tactattata ttaatttcag ccttatgcat ttttaatgga
    32581 ttattgactc ctggtgcata tgccgcaaca gcgaatggat acgtagcaaa taaagaaaat
    32641 ctacgtagtt tttttgaaac ggtttcatca tatgcgggta agcctacaat agtaagtaag
    32701 cttgccatga agaaacagat cagcggaaat tttgatttaa ccgaacccta tgccttgatt
    32761 gaacgcctgt cggcgcagat gggacttatt tggtacgatg atggcaaagc tatttatatc
    32821 tatgactcat cagagatgcg taatgcgctg attaatctga gaaaggtatc gacgaatgag
    32881 tttaataatt ttttaaaaaa atcgggtctg tataactctc gttatgaaat taaagggggc
    32941 ggcaatggta ctttctatgt atcggggcca ccagtttatg ttgacctggt cgttaatgct
    33001 gcgaaattaa tggagcaaaa cagcgatggc atcgagatcg gacgaaataa agtaggaata
    33061 attcatctgg tcaacacatt cgttaatgat cggacttatg aattacgtgg cgaaaagata
    33121 gttattcctg gcatggcaaa agtactatcg acgttgttaa ataataacat taagcaaagc
    33181 actggggtta acgtactttc tgaaatttca agtcgtcaac aactaaaaaa cgtatcacgt
    33241 atgcctcctt ttcctggtgc tgaagaagat gatgacctac aagtagagaa aataatatcg
    33301 acagctggag cgccagagac tgacgatata caaattattg catatcctga taccaacagt
    33361 ttacttgtta aaggaacagt atctcaggtt gattttatcg agaagttagt agctacgctc
    33421 gatattccaa agcgacatat tgaattatct ttatggataa tagatattga taagactgat
    33481 ctagaacagt taggagctga ctggtcgggc acgataaaaa ttggatccag cctaagtgca
    33541 tcatttaata attcaggttc aattagtacc ctggatggga cacagtttat tgcaacaata
    33601 caggcacttg cgcaaaaaag aagagccgcc gttgttgctc gtcctgtcgt tttaacacag
    33661 gagaatattc ctgctatatt tgataataac cgtactttct atactaaatt agtcggcgag
    33721 cgtactgcgg agttggatga agtaacctat ggtacgatga ttagcgtgct accacgattt
    33781 gcttcacgaa atcaaattga attactcttg aatattgaag atggcaatga aatcaattcc
    33841 gacaagacca atgttgatga tctgcctcag gttggtagaa cgcttataag tactattgcg
    33901 cgcgtgcctc aagggaaaag tcttttgatt ggtggatata cacgagatac gaatacctat
    33961 gaaagtcgta agatcccaat attaggcagt attccattta ttggtaaatt atttggatat
    34021 gaaggaacaa atgcgaataa catcgttcgt gtctttctta ttgagccaag agagatcgat
    34081 gagcgcatga tgaataatgc gaatgaggct gcggttgacg ccagagcaat aacacagcaa
    34141 atggcaaaaa ataaagaaat caacgatgaa ttactgcaga aatggataaa aacttacctg
    34201 aatcgtgaag tcgtcggagg atagtgaaat ggcgattcat gtagagcatg tcggtgtatt
    34261 agaaagagcc agagaagttt ctcgcttaga ggacattatc acagaagata atgaagatat
    34321 tgaagctgaa atgcctaaaa tgggggatga tcccgccggt aaagaagctc gattcttaca
    34381 agctactgac gagatgtcag cagcgttaac gcagtttatg aagaaaaaaa tctacgaaga
    34441 acaactggcc aactttctgg acggagaaga atatgtcctg gaagatcagc caatagaaaa
    34501 aaccgataaa gtcatggagg cgcttaaggc agcaactacc catgactatg aagtgtatag
    34561 ctttgctaaa aaattatttc cagatgaaag cgaactggtg gttgtattac gtgcaatttt
    34621 acgtaaaaaa caaatatctg aaaatgtgcg gctaaacgct gaagcattac ttaaaaaagt
    34681 taatcaagag actactaaaa aattcattaa tagcggtatt aactcggcat taaaagcaaa
    34741 gttatttggc caggcgttgt cattaaatcc aaaattacta agggcaagtt atcgacagtt
    34801 tttaatggca gaagacgatg ctgtagatac ttatgtcgaa tggattggta gttatggcta
    34861 tcaaaataga atactggtga caaaatttat caaagagacg cttttctcag atataaatgc
    34921 acttgatgcg agctgttcat cattggaatt tggtatgttt ctcaataagt tgtcacaact
    34981 attgtctctt cagtcagcag aggctttatt tctgaaaact ttaatgaata acccaataat
    35041 taaaaaattt attagcgcag aagattattg gatatttttt ctgatatcgt taataaaatt
    35101 tcctgagaca gcagaggaac tcctgaaaaa tgcactggta accctacccg cagatgctaa
    35161 ttataaagac aaaacgttac tcctgaaggc gatttacagc ggatgtacaa atcttccatt
    35221 ttcacttttt attaataatg aacaattatt agagattagg gagtgttgta aacaggcaat
    35281 aaaagtaaca tttgcagcag agctctttga tacacaaaac agtaataaaa aacaaaataa
    35341 aaaaccatgg aagaaggtaa tgttcaatgt ttaacaaagt tttgataggg ttaaggagtc
    35401 atccagaatt aataattctt ggattaatgg tgatgattat cgccatgctc ataattccat
    35461 tgcccaccta cttaatcgat tttttgattg ggctgaactt aacattggca atacttgtgt
    35521 ttttaggatc attctatgtt gataggatct taagtttttc ttcattccca tcaattcttc
    35581 tcatcacaac gttatttcgt ttggcgctgg cgatcagtac cagccgactt atacttcttg
    35641 aggcggacgc cggagaaatt ataacgagtt ttggtgaatt cgttattgga gatagcctgg
    35701 tagttggttt tgttattttc tccattgtta ctattgttca gtttatcgtg attactaaag
    35761 gttcggaacg tgtggctgaa gttgctgccc gcttttcgct tgacggtatg ccaggtaaac
    35821 aaatgagtat tgacgccgat ctacgcgccg ggatcattga tgcagattta gcaaaagaac
    35881 gtcgtagcgt attagaacga gagagtcagc tgtatggttc ctttgatggt gcgatgaaat
    35941 ttatcaaagg tgatgctatt gcaaacatta ttattatctt tgttaatatt atcggtgggc
    36001 tatcagtagg agtaggccaa aatggaatgg atttctcgac agcgttgacc gtctacacaa
    36061 ttcttaccgt cggtgatggt cttgtttcac aaattccagc tttattaatt gctattagtg
    36121 cagggtttat cgtaacgcgc gtgaatggcg atagtgataa tatggggcaa aatataatgt
    36181 cccagttatt aagtaattct tttgtcatta ttgtgacatg tgtcctcgcg ctaagtattg
    36241 ggttgcttcc aggttttccc cttcttgtgt ttttatgttt ggcagtaatc ttaggaattt
    36301 atttttactt taaattcaaa aagaaagggg ttgaagaaac ggttgttgag ggagatatca
    36361 ccgttgggtt ggaaccctat aatcaggatg atgatatttc attaggtatt attaataaac
    36421 ttgatcaggt tattaccgaa acggtaccct tggttttgat tatgaatagt gtacaggcta
    36481 aaaaatacac tgaaatcaat cttgctgata ggattcgtag tcagtttttt attgagtatg
    36541 gtatccgtat tcccggtatt gtcattcgcg agggggaagg ccttaatgat gaagatgtca
    36601 tattaatgct aaatgaagtc agagcctcgc aatttaaaat ttatcatgat ctcgtcttgt
    36661 tggttgaata ttcagatgaa gtggtttcga ctttgatcaa gaaacctatc atcgtaaata
    36721 gtaatggtga acaatattat tgggttacaa aaagtgatgc ccaaaaatta acaaaaattg
    36781 gctgttatac tcgcacggcc atggatgaaa tgtacaatca tttatctgta tgtctggcgc
    36841 ataatattaa tgagtatttt ggtatacaag aaaccaaata tattcttgat caattagaga
    36901 tgaagtatcc tgatctatta aaagaaatat taagatatat aaccgtgcag cgcatttcag
    36961 aggtaattca gcgtctgatc caggagcgaa tttcagtgcg caatatgcgt ttagttatgg
    37021 aggctttagc attatggagc ccacgggaga aagatataat tacactcgtc gagcatgtac
    37081 gaggtgcatt aggtcgctac atttgccata agttttcata ttctggagaa attaaagcaa
    37141 ttgtgatttc gcctgaaata gaggacagaa tcagagatgg tgtcaggcct accgctggcg
    37201 gtacttttct taatctcgat gcatccgaag ctgagatgat tctggataat tttaaactgg
    37261 ctctttctgg aatcaatatt cctattaaag acattatttt actgggatct gtggatattc
    37321 gtcgttttat caaaaaactt attgaatcta gttatcgaga cctcgaagta ctttcgtacg
    37381 gggaactaac ggaaaatgtt cccgttaata ttctgaaaac tatttaggat aatgtcttgc
    37441 gatatggagc atgctggtat gaaaaaatta aaactattaa ataaatacag ctacttacat
    37501 agcataaacg gctcgctgat tgaagcagag cttgacgacg tttcagtggg cgaagtatgc
    37561 gaaatttatg cgagctggca agcaaatgaa cgtatagcca gggctcaggt cgttggcttt
    37621 cgcaatggaa agacattatt aaaccttata ggcagctctg tggggcttac acgtactgca
    37681 gttttgaaac caacgggaga gcagttaacc atacagatca gtgatgcctt tcttggttca
    37741 gttttgaatg cttctggtca aattatggaa aggtttgtcc cggacactcc aggcgatagg
    37801 ggaaatttac gcctgattga tgaactgcca ccgtcgtatc aggagcgtcg agttattaat
    37861 acaccactag agacacggat aagagtcatt gatggcgtac tgacatgtgg cattggtcaa
    37921 cgtgttggga tttttgcatc agctggctgt ggcaaaacgg ttttaatgca tatgcttgta
    37981 aacaacactg aagctgatgt gtttgttatt ggcttgattg gcgaacgtgg aagggaagtt
    38041 acggaatgcg cggaatcgct gaaaaaatca gtaaatgcag caaaatgtgt tttggtttat
    38101 gccacctctg atttttcgtc agtagatcgg tgtaatgccg ccctgatggc gacaaccgtg
    38161 gcagagtatt ttagagatcg aggaaagaga gttgtacttt ttatcgactc aatgacgcgg
    38221 tatgcacgag cactacgtga tatgaaactg gcagcgggtg aaccacctgc cagacgtggt
    38281 tatcctgctt ctgtttttga cagtttgcct cgcttactgg agaggccggg accaacacta
    38341 aaaggaagta ttactgcgtt ttataccgta ttactggagg gggaggatga atcagaccca
    38401 ctgggtgatg aaattcgctc aatccttgat ggccatatct atctaagtcg caaactcgct
    38461 gggcaaggtc attatccggc aatagatgtt ctaaaaagtg tgagtcgagt atttggtcag
    38521 gtcactgatg aaaaacacag agataatgct gcacgggtaa ggaagatcct cacaactttg
    38581 gaagatctac aggtgtttat tgatttaggg gaatatcgcg ctgggcaaaa tgctgaaaat
    38641 gattttgcta tgaatgcccg gcctaaactc acaaattggt taaaacagtc agttaatgaa
    38701 aaaatgccaa tgagtgaaac gctaaaagag cttgaacgaa ttgttaagta aagttaatcg
    38761 acttatcaga agaacagcgc agagcctggc tgcatgtgaa gcatcattac aaaagttaaa
    38821 tgcagaaaaa gaaaagttag ccgaaaagga acgcctgtat gatatgcaac ttaaaaacct
    38881 acagtcactg ttagatatga aagagttact cggcgaagtt gtatttcgac aggatatatt
    38941 ttactcgtta cgtaaagttg cagtcattca gcagcagatc gctgagataa atcttgaaaa
    39001 gcaaaaaata gcagagcggc gaaaaatact taacaaggaa atagttcaac agcaagcaca
    39061 aagaaaacac tggtggctaa aaggagaaaa gtacgatcga ctaaagaagc gcatcaaaaa
    39121 acaactttta aatcagatgc tttatcaaga cgagcttgaa caggaggaga aatataatgg
    39181 acgaagtcaa gaaaattgaa cactgtaaaa ttttaaacac agacacaccc gataattctg
    39241 cttcagattt agacagtttc ttaaagaaaa acaaaaaaaa taaagaaatg agtgagattg
    39301 tcgctggaac cattctccag ttattacgtc agactcagga tagccaattt tctacgttgg
    39361 ggaaaaaaaa tgggcacatg gagtctattg acaacaaatc atctctccag gttgacagga
    39421 atccatctct ttctttgcaa acaaatggaa cagagaaatt ttctacaaag aaaattctca
    39481 atatgacttc acgagatcag gggaatgatt ctgaaccatc taataaaacg atacaattta
    39541 aggaaaaaat ccataatggt atccatacag aatacatcac agatcaaaaa cacagtaata
    39601 ataaagatcg tgaaaaaaaa taccgcgatg gagacaaaat aaatggaccg caggcccatt
    39661 cattagacat tactaacgaa agaaggtttg ctgataacag aaccatgttt actcaacata
    39721 tcgaaaagca aaggaatgtt catacgctca atcagaatga tataaataat tctgctaata
    39781 atgccaatgt gcgcgaaaat gaattgacat atcagtttca gcgttgggga caaaatcata
    39841 cagtccggat tctggaaagt agtgaaggga tacgactaaa accatcagac acacttgtct
    39901 ccgacagact ccatgaagcc caacacaatg atgttacagc acaacgctgg gtattgactg
    39961 agcaagatga acgacaaggg caacgacatc agcctcatga tgagcaagaa aatgaaggta
    40021 aattcgaaaa caatcagaag gacgaaagtt aatgttcggc ctgagaaaag taaatggaaa
    40081 cactcattcc tttgaaacca cttttcaaaa ctggaaagaa aatggtgaag atgttgcgtt
    40141 attaatgccc gaatttagcg ccaaatggct accgatagca gaagagagcg gcagctggtc
    40201 tggatgggtg ttacttcgag agttatttcc cttaatttct gccgagctcg ctgggatggc
    40261 tttaatgcca gaaacggaaa ggttaatagt ggaatggctc agtttatcta gtaccccgtt
    40321 aaatctaaag tatcctgaac taaaatataa tcgtttgtgt gtcggtaaag tatttgatgg
    40381 agtattgagt ccagctcaac cactgataag aatatggaca ggggaattaa atatttggtt
    40441 agataaagtc acagtctgcc aatacgaaaa cgctccaacg ctagacaaaa aatcattata
    40501 ttggcccatt cattttgtaa ttggatttag taaaacatgt tatagaacca ttgttgatat
    40561 tgaagttggt gatgttttat taatttcaaa taattttgct tatgcggtta tttataatac
    40621 aaagatttgt gatttaattt atccagagga gttaaaaatg gttgatcatt ttgagtacga
    40681 ggaagatttt gaaacagatg actttgatat caaaaaaagc gaaatcgaga ttaatgatga
    40741 aaatgactat cagcagatta atagttttga agacctgcct gtaaaaattg agtttgttct
    40801 tggtaagaaa ataatgaatc tatatgaaat agacgatctc tgtgcaaaaa gaattatatc
    40861 tttgcttcct gaagcggaga aaaatataga gatacgtgta aatggcgcgc taactggcta
    40921 tggcgaactt gttgaagtgg atgataaatt gggcgtagaa attcattctt ggttatcagg
    40981 gaataataat gtcaaatagc atttcgctga tagcgatatt atcattattc acactattac
    41041 cgtttataat agcatccggg acatgtttta ttaaattctc gattgttttt gttattgtcc
    41101 gaaacgcttt gggacttcag caagtgccat caaatatgac acttaatggg gtagctttgc
    41161 tattgtcaat gttcgtaatg atgccggttg ggaaggaaat atataataac agtcagaatg
    41221 agaatctttc attcaataat gttgcgtcgg ttgttaattt tgttgagaca ggcatgagtg
    41281 gttataaatc atatttgata aaatattctg aaccggagtt ggttagcttt tttgaaaaga
    41341 ttcagaaggt gaatagttct gaagacaatg aagagataat cgatgatgat aatatttcaa
    41401 ttttctcgtt actcccggca tatgcgttga gtgagataaa aagtgcattt attataggtt
    41461 tttatattta tttacccttt gttgtagttg atctggttat atccagcgtt ctgttaacgt
    41521 taggtatgat gatgatgagt ccggtaacca tatctacacc gataaaatta attctgtttg
    41581 tcgcgatgga cggttggaca atgttatcga aaggactcat tttacaatat tttgacctat
    41641 ccattaatcc ctgaggaaaa aatatggacg acattgtttt tgcggggaat agggcgctgt
    41701 atctgatttt agttatgtct gcaggaccta ttgcagttgc gacatttgtt ggtttattgg
    41761 tagggctttt tcaaacggta acacagttgc aagaacagac gttgccattt ggcgtcaaat
    41821 tattgtgcgt aagtatttgc ttttttttaa tgagcggatg gtatggtgaa aaattatatt
    41881 cgtttggtat cgaaatgctg aatttagcat tcgcgagagg atagcatggg ggaggcgatg
    41941 ctctatcagt tgcatagtct gctggctgct acagcgctcg gtttttgccg attggcacca
    42001 actttctatt tgttaccatt tttcgccagt gggaatatac caacagtagt ccgtcatcca
    42061 ataataatag tggtatcatg tgcattagtt cagcattacc actatgaatt atctacccta
    42121 aatgaaatag atatcgcttt gtttgctgcc agagaaatta tcatagggtt gtttattgct
    42181 tgcttattag catccccatt ttggattttc cttgctatag gtagctttat cgataaccaa
    42241 cgtggtgcaa cgttaagtag tactttggat ccggctacag gtgtcgatac atcggaatta
    42301 gcaagattat ttaacctttt ctctgcagca gtttatctta ctaacggtgg attgaacttt
    42361 attctggaga ctctatggca aagttataac ttatggcc
//
LOCUS       DAPOZO010000040        42269 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPOZO010000040 DAPOZO010000000
VERSION     DAPOZO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.040"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            67..1119
                     /gene="fbaB"
                     /locus_tag="ROI21_002218"
     CDS             67..1119
                     /gene="fbaB"
                     /locus_tag="ROI21_002218"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDX5111135.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1349..2203
                     /gene="gatY"
                     /locus_tag="ROI21_002219"
     CDS             1349..2203
                     /gene="gatY"
                     /locus_tag="ROI21_002219"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDX5111136.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            2232..3489
                     /gene="gatZ"
                     /locus_tag="ROI21_002220"
                     /pseudo
     CDS             2232..3489
                     /gene="gatZ"
                     /locus_tag="ROI21_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            3499..3951
                     /gene="gatA"
                     /locus_tag="ROI21_002221"
     CDS             3499..3951
                     /gene="gatA"
                     /locus_tag="ROI21_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDX5111137.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            3982..4266
                     /gene="gatB"
                     /locus_tag="ROI21_002222"
     CDS             3982..4266
                     /gene="gatB"
                     /locus_tag="ROI21_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDX5111138.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            4270..5625
                     /locus_tag="ROI21_002223"
     CDS             4270..5625
                     /locus_tag="ROI21_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDX5111139.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYLGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            5673..6713
                     /gene="gatD"
                     /locus_tag="ROI21_002224"
     CDS             5673..6713
                     /gene="gatD"
                     /locus_tag="ROI21_002224"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDX5111140.1"
                     /translation="MKSVVNDTDGIVRIAESVIPEIKHQDQVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMSIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMLTFNSLEMSAP
                     QMQGVLRELRFNQLILETAGVPKTVELAVEIAGPHAQLALVGTLHQDLHLTSTTFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            6819..7592
                     /locus_tag="ROI21_002225"
     CDS             6819..7592
                     /locus_tag="ROI21_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDX5111141.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(7674..8573)
                     /gene="yegS"
                     /locus_tag="ROI21_002226"
     CDS             complement(7674..8573)
                     /gene="yegS"
                     /locus_tag="ROI21_002226"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDX5111142.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VRIPEALDKALKLAIAGDAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            8988..9305
                     /locus_tag="ROI21_002227"
     CDS             8988..9305
                     /locus_tag="ROI21_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111143.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(9582..10595)
                     /locus_tag="ROI21_002228"
     CDS             complement(9582..10595)
                     /locus_tag="ROI21_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDX5111144.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     ETGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPTGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(10711..11010)
                     /locus_tag="ROI21_002229"
     CDS             complement(10711..11010)
                     /locus_tag="ROI21_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5111145.1"
                     /translation="MSIDVSEKLKLIRESERLNRKEFSELTGVAYSSLSSYESRSKNA
                     GVEAIMKVLQHPRFTKYTLWFMTDQVAPEAGQIAPALAHFGQNETTSPHSGQKTG"
     gene            11132..11407
                     /locus_tag="ROI21_002230"
     CDS             11132..11407
                     /locus_tag="ROI21_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HDX5111146.1"
                     /translation="MNRQLVSVTDAVPYQEFAKLIGKTPRAVRGMIEKGKLPVIEITD
                     PQSVSGRAGEYWVYIPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            11418..11588
                     /locus_tag="ROI21_002231"
     CDS             11418..11588
                     /locus_tag="ROI21_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111147.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRGWK"
     gene            11585..12085
                     /locus_tag="ROI21_002232"
     CDS             11585..12085
                     /locus_tag="ROI21_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HDX5111148.1"
                     /translation="MTVMTLNLVEKQPATMRRIIGKHLAVPRWQETCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWREALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            12149..12373
                     /locus_tag="ROI21_002233"
     CDS             12149..12373
                     /locus_tag="ROI21_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HDX5111149.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            12373..12675
                     /locus_tag="ROI21_002234"
     CDS             12373..12675
                     /locus_tag="ROI21_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HDX5111150.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLGLDKEKREITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKKLNDKRSW"
     gene            12675..12899
                     /locus_tag="ROI21_002235"
     CDS             12675..12899
                     /locus_tag="ROI21_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDX5111151.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDVPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRMA"
     gene            12896..13171
                     /locus_tag="ROI21_002236"
     CDS             12896..13171
                     /locus_tag="ROI21_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HDX5111152.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGH"
     gene            13161..15212
                     /locus_tag="ROI21_002237"
                     /pseudo
     CDS             13161..15212
                     /locus_tag="ROI21_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
     gene            15507..16658
                     /locus_tag="ROI21_002238"
     CDS             15507..16658
                     /locus_tag="ROI21_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020316745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDX5111153.1"
                     /translation="MNVVDLFSGVGGLSLGATRAGFNLVGAVELDKHAIFSHELNFPK
                     STHLHRDVCSLTAKDILSACAVKDIDCVIGGPPCQGFSTIGKRNANDTRNELYIHFFR
                     LVSELSPICFLAENVPGIMGEKYNPVREEAFSLVRDGYCLLHPIRVNASNYGAPTTRT
                     RIFFMGFKRGFINQLKESDFFPKNIIEQTLVKDALYGLPRTIRKEWQTEEQGWRSVKM
                     DKDGYYYERLWGHIPNGVGDAESIQNLKKGLVSGFLGTAHSEEVLRRYRQLSFGETDK
                     ISRSQRLNPNGFCPTLRAGTGSDKGSYQAVRPIHPTQARVITPREAARLQGFPDWFRF
                     HPTKWHSFRQIGNSVSPLVAEAMLLPLYDYCLKVKKESLNTMERPCSIR"
     gene            complement(16609..17601)
                     /locus_tag="ROI21_002239"
     CDS             complement(16609..17601)
                     /locus_tag="ROI21_002239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111154.1"
                     /translation="MSTGGSIPYHLRQNKAIERNLFIESLRRLNNYTNISEYVYIGFG
                     GPFLEDFKQIHNLLKVTKMISIEGDKNVHCRQQFNKPLSCINLGDTPEMSGDFINRYN
                     FDDKTIIWLDYATPSDLNAQLNEIVNLVTKLKPKDIFKITLNANPEALGKNPSERDPR
                     AYRFQKINDILTQDFLPPNAVVEDVGFKKYPMLLVNAMRRAIGHGLKGRNDIRIHPLT
                     SFVYKDGQQMVTLTAIVLENTDTEEAKFIDNSRIKNWPFYDGKWSNPKNINVPAMSLK
                     ERIHIESLLPEASVENIHETLGFYIGSTPSGANSDLSNFIEYYKVVPWYSKILF"
     gene            complement(17598..19022)
                     /locus_tag="ROI21_002240"
     CDS             complement(17598..19022)
                     /locus_tag="ROI21_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020316734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDX5111155.1"
                     /translation="MVSSNVKIAAFPAKRFFVEMLTRDIELSDSILDLLDNCLDGVLR
                     KNNFTPEQTFGKSDVYKGYYAHIEFDENSFRITDNCGGIPAKLAESYAFRLGRPAERE
                     ADDLPTIGVYGIGMKRAIFKMGTSAQILSKTENEQFSVTISPEWMADDENWSLELERN
                     NVDLNETGVSIQINNLRKDIKASLSKDRGFEEVLINIIANHYSLIIKKGFEVKVNGCI
                     VKPNSTNLIFDEEAIKGNTDGIAPYVYKNNSNGVSIKVAVGFYRNLPSEEEEENLLSG
                     RSTTEKAGWTIICNDRVVLHADKTKLTGWGEAGVPQYHTQFIGISGVVIFTSSNAELL
                     PITTTKRGVDGNSELYLSTKDFMREGLKFFTDFTNKWKSNAEERKQIINTASNMVSTT
                     EVDFTKSIPRENWSNVRRSIGGEVFKPKLPMPRDSDPLRQIKFSRRHSEIKLVSEFLF
                     DDATQPPTEVGQYCFDEFLKKAKQ"
     gene            complement(19409..20443)
                     /locus_tag="ROI21_002241"
     CDS             complement(19409..20443)
                     /locus_tag="ROI21_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDX5111156.1"
                     /translation="MSKKKGKTPQPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFTPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDND"
     gene            complement(20443..22215)
                     /locus_tag="ROI21_002242"
     CDS             complement(20443..22215)
                     /locus_tag="ROI21_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDX5111157.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            22389..23243
                     /locus_tag="ROI21_002243"
     CDS             22389..23243
                     /locus_tag="ROI21_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDX5111158.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEVETAFSALKQEVTDKVDETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            23302..24375
                     /locus_tag="ROI21_002244"
     CDS             23302..24375
                     /locus_tag="ROI21_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDX5111159.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGITGPIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQVNFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGRTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            24379..25122
                     /locus_tag="ROI21_002245"
     CDS             24379..25122
                     /locus_tag="ROI21_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDX5111160.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLELTAAAD
                     MPDEVRAKLHKITGLFLRDGGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRATRKTRSVTPAKRGRPKKKAS"
     gene            25222..25731
                     /locus_tag="ROI21_002246"
     CDS             25222..25731
                     /locus_tag="ROI21_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDX5111161.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQG
                     KPRCIVSQI"
     gene            25731..25934
                     /locus_tag="ROI21_002247"
     CDS             25731..25934
                     /locus_tag="ROI21_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDX5111162.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            25938..26219
                     /locus_tag="ROI21_002248"
     CDS             25938..26219
                     /locus_tag="ROI21_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDX5111163.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            26219..26716
                     /locus_tag="ROI21_002249"
     CDS             26219..26716
                     /locus_tag="ROI21_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HDX5111164.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            26731..27156
                     /locus_tag="ROI21_002250"
     CDS             26731..27156
                     /locus_tag="ROI21_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysA"
                     /protein_id="HDX5111165.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVATWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRKACRQRLEGRELCQS"
     gene            27144..27569
                     /locus_tag="ROI21_002251"
     CDS             27144..27569
                     /locus_tag="ROI21_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HDX5111166.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASEQQ
                     TTITMLKNQLHVAITRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACADASDCPQRLPESESLPDAGQ"
     gene            27556..27714
                     /locus_tag="ROI21_002252"
     CDS             27556..27714
                     /locus_tag="ROI21_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein"
                     /protein_id="HDX5111167.1"
                     /translation="MPGSDPQKNGDLSADIRLLENALARCASQVKMIKHCQDENDAQT
                     RQPAQGAD"
     gene            27677..28144
                     /locus_tag="ROI21_002253"
     CDS             27677..28144
                     /locus_tag="ROI21_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5111168.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            28137..28589
                     /locus_tag="ROI21_002254"
     CDS             28137..28589
                     /locus_tag="ROI21_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDX5111169.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSTELAKHLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLER"
     gene            complement(28661..29446)
                     /locus_tag="ROI21_002255"
     CDS             complement(28661..29446)
                     /locus_tag="ROI21_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111170.1"
                     /translation="MVSYNVTNVWGLIVFFLCSFAVLAFFSFGKSNLMRLIAHYLNFG
                     YSDKKLKRLDREWRDIQLFKIINGINVSGIENVRMIQQGLIDGKLKTSYFFLTRIWGD
                     ITKPPHVIKTIIVILASIFYILLACYIHNEQSVIVRDAIGIPYKNMMYYVYSDKVLLS
                     FKNKAVEFNKTYSLADCKRLQNVFIKDTLPEIACNKLLQLNEEDSEWLSQEIKDNNSH
                     KKALLILSLVYFTSGLVIFLSYTKFFYANKKVLEYKASNKNHS"
     gene            29530..30165
                     /locus_tag="ROI21_002256"
     CDS             29530..30165
                     /locus_tag="ROI21_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDX5111171.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPPPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVMVKASTRVTLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            30162..30509
                     /locus_tag="ROI21_002257"
     CDS             30162..30509
                     /locus_tag="ROI21_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDX5111172.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTERSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            30514..31422
                     /locus_tag="ROI21_002258"
     CDS             30514..31422
                     /locus_tag="ROI21_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HDX5111173.1"
                     /translation="MPIIDLNQLPAPDVVEELDFESILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            31415..31945
                     /locus_tag="ROI21_002259"
     CDS             31415..31945
                     /locus_tag="ROI21_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDX5111174.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            31956..33977
                     /locus_tag="ROI21_002260"
     CDS             31956..33977
                     /locus_tag="ROI21_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5111175.1"
                     /translation="MSTKFKTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHALNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVDLTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKTVMDETNKKAPLNSPALTGT
                     PTTPTARQGTNNTQIANTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTN
                     ALAGKQPKDATLTALAGLATAADRFPYFMGNDVASLATLTKVGRDILAKSTVAAVIEY
                     LGLQETVNRAGNAVQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSFM
                     WFETGDNGNEYFKWRSRQSTTTKDLMNLKWDALYVLVNAIVNGEVISKSANGLRIAYG
                     NYGFFIRNDGSNTYFMLTNSGDNMGTYNGLRPLWINNATGAVSMGRGLNVSGETLSDR
                     FAINSSNGMWIQMRDNNAIFGKNIVNTDSAQALLRQNHADRKFMIGGLGNKQFGIYMI
                     NNSRTANGTDGQAYMDNNGNWLCGSQVIPGNYGNFDSRYVKDVRLGSQQYYGVNNWQT
                     WNFQCPSGHVLSGINVQDTGSNSADNIAGVYYRPVQKYINGTWYNVASV"
     gene            33979..34506
                     /locus_tag="ROI21_002261"
     CDS             33979..34506
                     /locus_tag="ROI21_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDX5111176.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDENNIIVAITRDASTLNPEGFSVVEVPDITSNRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERARLESWERYSVLV
                     SRVDPVNPEWPEMPQ"
     gene            34776..35321
                     /locus_tag="ROI21_002262"
     CDS             34776..35321
                     /locus_tag="ROI21_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogenic conversion protein"
                     /protein_id="HDX5111177.1"
                     /translation="MILAMVLFVGNIFYTNHRDDISMEAERDSIRTMFAYEIANNHRA
                     LTFLDKTRYIGFDENSEHFVGEPFAINVKSLGGPRLQIALNQTDKVFKSYFSELSKLD
                     KEDVTLLMDYYHEQSILLERVKSTLQKMKSGNDIKVDIDGYLLEEHFMNELNLSNILL
                     KRYSHLLSQYAKEHKTKDLHN"
     gene            35651..36841
                     /locus_tag="ROI21_002263"
     CDS             35651..36841
                     /locus_tag="ROI21_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HDX5111178.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAEIFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAQTLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            36854..37372
                     /locus_tag="ROI21_002264"
     CDS             36854..37372
                     /locus_tag="ROI21_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDX5111179.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            37429..37704
                     /locus_tag="ROI21_002265"
     CDS             37429..37704
                     /locus_tag="ROI21_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDX5111180.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            37737..37856
                     /locus_tag="ROI21_002266"
     CDS             37737..37856
                     /locus_tag="ROI21_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDX5111181.1"
                     /translation="MADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE"
     gene            37849..40296
                     /locus_tag="ROI21_002267"
     CDS             37849..40296
                     /locus_tag="ROI21_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDX5111182.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLALTIGIIVGGALALIGVIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATRDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESGTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            40311..40790
                     /locus_tag="ROI21_002268"
     CDS             40311..40790
                     /locus_tag="ROI21_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5111183.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAFQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSLATRFTGMMGAG"
     gene            40790..41953
                     /locus_tag="ROI21_002269"
     CDS             40790..41953
                     /locus_tag="ROI21_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDX5111184.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVRQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            42035..42253
                     /gene="ogrK"
                     /locus_tag="ROI21_002270"
     CDS             42035..42253
                     /gene="ogrK"
                     /locus_tag="ROI21_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HDX5111185.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
BASE COUNT        10601 a         9988 c        11188 g        10492 t
ORIGIN      
        1 gcccgcaaaa ggcgggccag gacaatccat agccgatatc caatcggaat ttacgggagc
       61 atagtaatga cagatattgc gcagttgctt ggcaaagacg ccgacaacct tttacagcac
      121 cgttgtatga ctattccttc tgaccagctt tatctccccg ggcatgacta cgtagaccgc
      181 gtgatgattg acaataatcg cccgccagcg gtgttacgta atatgcagac gttgtacaac
      241 actgggcgtc tggctggcac aggatatctt tctattctgc cggttgacca gggcgttgag
      301 cactctgccg gagcttcatt tgccgctaac ccgctctact ttgacccgaa aaacattgtt
      361 gaactggcga tcgaagcggg ctgtaactgt gtggcgtcaa cttacggtgt gctggcatcg
      421 gtatcgcggc gttacgcgca tcgcattcca ttcctcgtca aacttaatca caacgagacg
      481 ctaagttacc cgaataccta cgatcaaacg ctgtatgcca gcgtggagca ggccttcaac
      541 atgggcgcgg tggcggttgg tgcgactatc tattttggtt cggaagagtc acgtcgccag
      601 attgaagaaa tttctgcggc ttttgaacgt gcgcacgagc tgggcatggt gacagtgctg
      661 tgggcctatt tgcgtaactc cgcctttaag aaagatggcg ttgattacca tgtttccgcc
      721 gacctgaccg gtcaggcaaa ccatctggcg gcgaccatag gtgcagatat cgttaaacaa
      781 aaaatggcgg aaaataacgg cggctataaa gcgattaatt acggttatac cgacgatcgc
      841 gtgtacagca agttaaccag cgaaaacccg attgatctgg tgcgttatca gttagctaac
      901 tgctatatgg gccgggcagg gttgataaac tccggcggtg ctgcaggcgg tgaaactgac
      961 ctcagcgatg cagtgcgtac tgcggtcatc aacaaacgcg ctggcggaat ggggctgatt
     1021 cttggacgta aggcgttcaa gaaatcgatg gctgacggcg tgaaactgat taacgccgtg
     1081 caggacgttt atctcgatag caaaattact atcgcctgat gtgctttgta cgaccagcac
     1141 tgccacgtat gacggataag gcgtttacac tgcatccggc ataaaataac gcgcactttg
     1201 ctacggcttc cctatcggga ggccgttttt ttgcctttca ctcctcgaac aattttcata
     1261 ttgtcgtttt tgtgatcgtt atctcgacat ttaaaaacaa ataatttcat tatattttga
     1321 aatcgaaaac aaacgacagg atatgaaaat gtacgtggta tcgacaaagc agatgctgaa
     1381 caacgcacag cgcggcggtt atgcggttcc ggcattcaat attcacaatc tcgaaacgat
     1441 gcaagtggtg gtagaaaccg ctgccaacct gcatgcgccg gtcatcatcg ccggaacgcc
     1501 tggcacattt actcatgccg gtacagaaaa tttgttggcg ctggtcagcg cgatggcgaa
     1561 gcaatatcac catccactgg cgattcatct cgaccatcac acgaaatttg acgatatcgc
     1621 tcagaaggtt cgttctggcg tgcgctcagt catgattgac gcctcgcatt tgccttttgc
     1681 gcaaaatatt tcacgggtca aagaggtggt ggatttttgc catcgctttg atgtcagcgt
     1741 cgaagcggag ctggggcaac ttggcggcca ggaagatgat gtgcaagtca atgaagccga
     1801 tgctttttat accaaccccg ctcaggcgcg tgaatttgcc gaggcaaccg gaattgattc
     1861 cctggcggtc gccatcggca cggctcatgg tatgtatgcc agcgcaccgg tgcttgattt
     1921 ttctcgactg gagaacattc gccagtgggt gaacttaccg ctggtgctgc atggcgcgtc
     1981 aggattatcg actaaggata ttcagcaaac catcaaactg gggatatgca aaatcaacgt
     2041 tgcaacggag ctgaaaaatg ccttctcgca ggcgttaaaa aattacctga ccgagcaccc
     2101 cgaagcgacc gatccccggg attatttgca gtcggctaaa tccgcaatgc gcgatgtggt
     2161 gagcaaagtg attgccgatt gtggctgcga gggcagggca taacgcacct gccatttaac
     2221 aaggaaaaaa catgaaaacg ttaattgccc ggcataaagc tggtgaacat atcggcatat
     2281 gttcagtctg ttctgcccat ccgttggtta tcgaagcggc gctggcattt gatcgcaaca
     2341 gcacgcgcaa agtgctgatt gaagcaacgt caaaccaggt caatcaattt ggcggttata
     2401 ccggaatgac accggcagac tttcgcgaat ttgtttttac gattgccgat aaagtcggat
     2461 ttgctcgtga gcgtattatt ctcggcggcg accatctggg gccaaactgc tggcagcaag
     2521 aaaatgcgga tgcggcgatg gaaaaatccg tcgagctggt aaaggcatat gttcgtgctg
     2581 gcttcagtaa aattcatctt gatgcgtcaa tgtcctgcgc gggggatcct ataccgttag
     2641 caccagaaac ggttgcggaa cgagctgctg tgctttgctg cggaaagtgt ggcgacagat
     2701 tgccagcgtg agcaactgag ctatgtcatt ggcaccgaag ttccggttcc gggcggtgag
     2761 gccagcgcca ttcagtcagt acacattacc cgggttgaag atgccgccaa tactttacgt
     2821 acgcatcaaa aggcctttat tgcccgtggg ctggcagagg cgttaacacg cgtgattgcc
     2881 attgtggtgc agccaggcgt ggaatttgat cacagcaata ttatccatta tcagccgcag
     2941 gaagcacagc cgctggcgca atggatagaa aacacccgaa tggtttatga agcacattct
     3001 accgattacc agacccggac ggcttattgg gaattagtcc gcgatcactt tgcaatattg
     3061 aaagtcggtc ccgcattaac ctttgcttta cgtgaggcga tatttgcgct ggcgcaaatt
     3121 gagcaggaac ttatcgcccc agaaaatcgc agcggttgcc tggcggtaat tgaagaagtg
     3181 atgtttgacg aaccgcaata ctggaaaaaa tattatcgta cgggttttaa cgattcatta
     3241 ctggatattc gttacagcct gtcggatcgt attcgttatt actggccgca tagtcggatt
     3301 aaaaatagcg tcgaaacgat gatggtgaat ctggaaggca tggaaatccc tctgggcatg
     3361 attagtcagt atcttcctaa acaatttgaa cgcattcagt ccggggaatt atcagcaata
     3421 ccgcatcagt tgattatgga taaaatttat gatgttttgc gcgcctatcg ctacggctgt
     3481 gcggaataag gacggtatat gactaacctg tttgttcgta gcggaatttc ttttgtcgat
     3541 cgtagcgaag ttttaaccca tatcggtaat gagatgctcg cgaaaggtgt ggttcatgat
     3601 acctggccac aggcattaat tgccagagaa gcagaattcc ctaccgggat aatgcttgag
     3661 cagcacgcca ttgcaatacc gcattgtgag gcgattcatg ctaagtcgtc agccatttat
     3721 ctgttaaggc caacaaataa agttcatttt cagcaagcgg atgatgataa cgacgtggcg
     3781 gtatcgttgg ttattgcgtt aattgtggaa aatccgcagc agcaattgaa acttttacgc
     3841 tgtttatttg gcaagttaca acagcccgag atcgtcgaga cactaatcac tcttcctgaa
     3901 acccagttaa aggaatactt cacaaagtat gttttagatt cagacgaata aatccctctg
     3961 taacaataaa aaggactatt tatgaaacgc aagattattg tcgcttgcgg aggcgcggtt
     4021 gcgacctcca cgatggcggc ggaagaaatt aaagagttgt gtcagagtca taatattcct
     4081 gttgaattaa tccagtgtcg ggttaatgaa atagaaacct atatggatgg cgtgcatttg
     4141 atatgtacta ccgccaaagt tgaccgtagt tttggcgata ttccgttagt tcacggcatg
     4201 ccttttgttt ctggtgtcgg tatcgaagca ttacaaaata aaattctgac tatcttacag
     4261 gggtgaccta tgttttcaga agtcatgcgt tatattctcg acctcggccc tacggtgatg
     4321 ctgccgattg tcatcattat tttttctaaa atattaggca tgaaggcagg cgattgcttt
     4381 aaagcgggtc tgcatatcgg gattggcttt gttggcattg gccttgtgat tggcttaatg
     4441 ctggattcca ttggcccggc ggcgaaagcg atggcggaaa atttcgacct gaatctgcat
     4501 gtggtcgatg tcggttggcc gggctcttca ccaatgacct gggcgtcgca aattgcgctg
     4561 gtggcgattc cgattgcgat tctggttaac gtggcgatgc tactgacccg tatgacgcgg
     4621 gtggtaaatg ttgatatctg gaatatctgg catatgacct tcaccggcgc attgctgcat
     4681 ctggcaaccg gttcatggat gatagggatg gcgggtgtgg taattcacgc ggcgtttgtt
     4741 tataagctcg gcgactggtt tgcccgcgat acccgaaatt tctttgagct ggaaggcatt
     4801 gccattccgc acggtacgtc ggcgtatctg gggccaattg cggtgctggt ggatgctatc
     4861 atcgagaaaa tcccaggcgt taaccgtatt aaatttagcg ccgacgatat tcagcgcaaa
     4921 tttggcccat ttggcgagcc tgtcaccgtg ggttttgtga tggggctaat tatcggcatc
     4981 ctcgcgggtt acgatgtcaa aggtgtattg cagctggcgg taaaaacggc ggcagttatg
     5041 ctgctgatgc cacgggtgat taaacccatc atggatggtt taacgcccat cgctaagcag
     5101 gcccgtagtc gtttacaggc gaagttcggc ggtcaggagt tcctgattgg ccttgatccg
     5161 gcgttgctgc tggggcatac ggcggtggta tcggcaagcc tgatttttat tccactcacc
     5221 attttaattg ctgtttgtgt gccgggtaat caggtgctgc cgtttggcga tcttgcaact
     5281 atcggcttct tcgtggcgat ggcggtcgcc gtgcatcgtg gaaatctgtt ccgcacctta
     5341 atctcgggtg tcatcattat gagcatcacc ctgtggatcg cgacgcaaac tattggtttg
     5401 cacacccaac tggcggccaa tgctggggcg ttaaaagccg gaggtatggt ggcttcaatg
     5461 gatcagggcg gttctcccat tacctggtta ctgattcagg ttttctcccc gcaaaatatt
     5521 cccggtttca ttattatcgg cgcaatttat ctgaccggta ttttcatgac ctggcgtaga
     5581 gcgcgtggtt ttattaaaca agagaaagcc gttctcgcag aataattttt acctgagggg
     5641 gaggtaatcc cccttaacaa tcaggagttt ttatgaaatc agtggtgaat gatactgatg
     5701 gtatcgtgcg cattgcagaa agcgtcattc ctgaaattaa acatcaggat caggtgcggg
     5761 taaaaattgc cagctcgggc ttatgtggtt ccgatttacc caggatattt aaaaatggtg
     5821 cacattatta tccaataacg ttaggccatg aatttagcgg ctatattgat gcggtgggat
     5881 ccggtgttga tgatttacac cctggcgatg cggttgcctg tgtgccgtta ttaccctgtt
     5941 ttacttgtcc agagtgtttg aaagggtttt attcccagtg cgcaaaatat gattttattg
     6001 gctcgcggcg tgatggtgga tttgctgaat atattgtcgt taagcgaaaa aatgtctttg
     6061 ctctacccac ggatatgtct attgaggatg gggcttttat tgagccgatt accgttggcc
     6121 tgcatgcttt tcatttagcg caaggttgtg agaataaaaa cgttattatt attggtgccg
     6181 gaaccattgg cctgctggca attcagtgcg ctgtcgcgct gggagcaaag agtgtgacgg
     6241 ctatcgacat tagctcagaa aaactggctc tggcaaaatc tttcggggcg atgctaacat
     6301 ttaacagcct tgaaatgagc gcgccgcaaa tgcagggcgt tttacgcgag ctgcgcttta
     6361 atcagcttat cctcgagacg gctggtgtcc cgaaaaccgt cgaactggcg gtagagattg
     6421 ccgggcctca tgcccagctg gcgctggtgg gcacgttgca tcaggatctg catttaacat
     6481 cgacaacgtt tggcaaaata ttacgtaaag agctgacggt tatcggcagc tggatgaact
     6541 actccagccc ttggccgggg caggagtggg aaacggcgag ccggttgctg acagaacgta
     6601 agttaagcct ggagccatta atcgctcacc gtggaagctt tgaaagcttc gcccaggcgg
     6661 tgcgtgacat cgctcgtaat gctatgccgg gcaaagtgtt gctcattccc tgaaaccgcg
     6721 ggccagcgtg atgctggccc ggtattgtgc aaaacagatc attcaccaat ggtccccctt
     6781 cgtttacact agccacaatt gaatatgggt aaatgacgat gaattcattc gagcgaagga
     6841 ataagatcat ccaattagtg aatgaacagg gaaccgtgct tgttcaggat ctggcgggag
     6901 tatttgctgc ctcggaagcg acaatccgtg ccgatttgcg ctttctcgaa caaaaaggcg
     6961 tggttacgcg ctttcatggc ggtgcggcga aaataatgtc cggtaatagt gaaaccgaga
     7021 cccaggaagt tgggtttaaa gagcgatttc agctcgccag cgcgccaaaa aacagaatag
     7081 cgcaggcggc agtcaaaatg atccacgaag ggatgaccgt tattctcgac agcggaagta
     7141 cgacaatgct tatcgccgaa ggattaatga ccgccaaaaa tatcacggtg attaccaaca
     7201 gtctcccggc agcatttgcc ctttccgaaa ataaagacat tactctggtc gtctgtggtg
     7261 ggaccgtccg ccataaaaca cgctcgatgc atggttccat tgccgagcgt tcattgcagg
     7321 atattaatgc cgatttaatg tttgtcggtg ctgatggtat tgatgcggtt aatggtatta
     7381 cgacctttaa tgaaggttat tcgattagcg gggcgatggt tacagccgca aataaagtga
     7441 ttgccgttct cgattcatcg aaatttaatc gtcgcggttt taatcaggta ctgccaatcg
     7501 aaaaaattga cattattatt accgatgatg ccgtgtcaga ggtggataaa ctggcattgc
     7561 agaagacacg ggtgaaatta attacggtgt agtattgctc tgtatgccgg ataaagtttt
     7621 tacaccgcat ccggcattac ggattctccc tggttcaaga gagaatagtg tgattaacgc
     7681 aacaatgggc aatctggtgg taatcgacaa cgcaacgccg ccggaagtat ttcaatatga
     7741 aagttttgtc cgctcaacgg ttcgccatca agattaaaag tgatttcgtg tggggcttgt
     7801 atatcaaacc acgacgaagc gccttcgata atattcgggt tatcttcgtc agattttaat
     7861 gttgatacga gagccggaag aatttcatcg ccggtaaaaa tgcgcagttg cagcaagcca
     7921 tcgttaatta acgcgttcgg gcacaattgc tgaccgccac cggcctgacg cccgttacca
     7981 ataccaatga ccagggcgtc accttgccag tgaaagtttt caccgcggat ctcacaacgg
     8041 tccggttgca gagtatccat gcgcattaag ccatgaatga tgtaagagac gccacccagc
     8101 gcggctttta atttttccgg cgtttctgtg gtaatacgcg tcccaaatcc gcctgtcgcc
     8161 atattaataa aacaggtttg tttgttgacc tgcgccatat ctatcgcaat ggcgtcaccg
     8221 gcaattgcca gtttcagcgc cttatccagt gcctcaggaa tccttacact ggtggcaaaa
     8281 tcattggcgg ttcctaatgg caaaattcct agcgcgggta tgtcatcccc ctcacactga
     8341 atcaacgccg tagaaacttc attaatggtg ccatcgccac caccggcaat caccgttgcg
     8401 acgcccaact tccgggcctc ctctacatat cgtgcggcat cgcctttctc ccaggtgacc
     8461 cgcacatgga tcgtcattcc ttcctcacgc aacagcataa ttgcttcgcg caatggtaaa
     8521 ttgtcagtac ttttgccatt aagaatcagt aagctggcgg gaaattctgc catgatcgta
     8581 tgtgccttta tgattggtct gtagatagtg tagagcagaa aagaaaaggt ggagtcagaa
     8641 caggatgaaa gtcggaggat aaaaaatcag ttcaagtatg gaggattcag tggttaccac
     8701 caggagcttg caatggggac agtgaatgtt aacggtagcc accggtaaat ttcaaaaaag
     8761 agcatatacc taatattcaa ctaaacagtg gcatcttcac tatcttattc taaatatatt
     8821 aaagccctca tggggatacc tgggcagggc ttcatgtttg taattcctac ttatgtagga
     8881 aatgttgtac agaacattta ttataatcct attcaattat aataatcatg ccattattat
     8941 attttaacac cagagcgtgt cgttggtatt taatggggga aggtaagatg aaaaagatag
     9001 ctgctatatc attaattagt gtttttctta tgtctgggtg tgctgtacat aatgatgaga
     9061 caagtatcgg taaatttggt cttgcatata aaagtaatat tcagcgtaaa ctcgataacc
     9121 aatactacac cgaagccgaa gcttctttag ccaggggcag aatatctggt gcagaaaata
     9181 tagtaaaaaa tgatgcagct catttctgtg ttactcaggg caaaaaaatg cagatagttg
     9241 acctgaagac agaaggtgca ggattacatg gcgtcgctcg tctgacattc aaatgtggag
     9301 agtgagaata ttttttggta agcgtcaaac atgcgcgttc tggttgtgct tagccggaac
     9361 ctgtgcgagc acgatgccgt tacgtgaaag gcatcgtgct atgaagggag attctatcga
     9421 tgtggtcaat ggaagacggt cacctgggat caatccgtct ctatggttta tgcacaaaaa
     9481 ataagcccgt gtaagggaga tttagggtgt caccagtagg ggctttcaac ggtacaatgc
     9541 gggtttgagc ggcataaatt accactgaaa gcccttaaac gttactctac tgtggacact
     9601 gtgtggacac tctcggcttc agtaccacct cttagcggat taagagaaat ggcgtcctga
     9661 aggtactctg gcgcaaaatg agcgtaaacc atagtttgct caatccgcgt gtgacctagt
     9721 atccgttgta gcgtgataat acttcctcca ttaatcatga aatgagtggc aaagctgtgc
     9781 cttagtgcat gtgtggcttg ccccgttggc aaatccggtt ttattgcttt cattgttcgt
     9841 ctgaagcgag ggtaatcagc atcagggaat aaaaaacctc gtttgttatc cgcgatcatt
     9901 ttggcaacag cctctgagat cgggacggtg cgtggtttgt ttgttttcgt tttaacaaac
     9961 gtgacgcggt tatggatgat attttctgct ttcaaacgag ctgcttctcc ccaacgtgct
    10021 cctgtactca ggcaaagaat cgcaatcttt ttattgtcgc cgtcaagtgc tgcaagcagt
    10081 aaggcaattt cttcctgtgt gagatagcct gtttctggtt tttcctcctt aagcctcttt
    10141 gtccctctga tagggtgctc accaaagaat aactccgctt caatcagggc tgtaaacatg
    10201 ccgctaatac atgttaaatc acgattgata ctcgaaggtt taataccctg acttcttcgg
    10261 gtggcgcagt actggctgat aagggatttc gtgatttgaa atgcgcatgg gtcattcgtt
    10321 atttttgtga agatttcaat ttttccaaga ttagatttcc catgctcttc gtgtttaccc
    10381 tttaaatccc accagatctg tgtcagttcc gacagacgtc gtttgtctgt tggttttgat
    10441 agccattctt tattgtggtg gttgtacaac gtgtattttt cgaaagcgac agcttcgctt
    10501 ttcttatcaa acttcctacg gatgcgtttt ccgttacgtc cagtagggcg aatgtccact
    10561 tcatatcgac catcatcgag ttttttgatt gccatcagaa aaccctccga gtggtacttt
    10621 tttttgctac tactaatcgc ttttttcgtg gtggctgaaa tttagccacc aatagtaggc
    10681 acttgtgatg aatatattca cgataaattg ttaaccagtc ttttgaccgg agtggggcga
    10741 cgttgtttcg ttttgcccaa agtgtgcgag agcgggcgca atttgcccgg cttctggagc
    10801 tacctgatca gtcatgaacc acaaagtata tttagtaaat ctgggatgtt gtaagacctt
    10861 cattatggct tcaactccag cgttttttga ccggctctca tagctcgaaa gtgagctgta
    10921 ggctacacca gttaattcac tgaattcttt acggtttaac ctttcagatt cacggattag
    10981 cttcaacttc tccgaaacgt ctattgacat aattactccg attgcgtaat ttcttgctga
    11041 tggtgtgaaa tgttgtgctt ctggagttat ccttttaggc aataattagc cattaggagc
    11101 cattagaagc actaagggag aatcgtagca gatgaataga cagcttgtaa gcgtgactga
    11161 tgccgtgcct tatcaggagt ttgcaaaact cattggtaaa actccaagag ctgtaagggg
    11221 catgattgag aaagggaaat taccagttat tgagattact gaccctcagt cagtatcggg
    11281 gcgtgctggt gaatattggg tatacattcc ggcatggaat aacggactaa aactggctta
    11341 tgaaagccgt cctaaagaga ttcgtgacgg ctggttgatg tggttaggtc tcggtgaacc
    11401 acgttaagga gaaccgtatg aatgagcctc gttgtattgc tcagttattg cgtaacgaaa
    11461 gccccagggc gattgacttc accatcaccc acgggaaggg acgcaaggga atcattatcc
    11521 gcaccaaaaa acagagtccg ttaaagaagg ctctgacctt tctgaaaagc cggaggggct
    11581 ggaaatgaca gtgatgacgc tcaatctcgt tgaaaaacag ccagcaacta tgcgccggat
    11641 aattggtaag catcttgccg ttcctcgctg gcaggagaca tgtgattatt ataatcagat
    11701 gatggagcgc gaacggctaa cagtttgctt tcatgcgcag ttaaaacaac gtcacgcaac
    11761 gatgcgtttt gaagaaatga acgacgtcga acgtgaacgg ctggtttgtg caattgatga
    11821 attgcgtggg gcattctcaa aacgccgtca ggtcggtgca agtgagtatg catatattag
    11881 ctttttaact gtcagtcagc gccgcacttt atttatgcac gcacgactga cagaaaaaga
    11941 atttaaccag ccatactggc gaattaatga agaatcatgt tactggcgtg aagctttatt
    12001 ccgtgcatta cgtgaattat tcagtctgtt tgagtatgca ccgacaattc tgacatcggt
    12061 aaaaccagag caatatctgc attaagtaat taaccagagt ttttaacgca cttaattgtg
    12121 cggggcttct ttttgcctgg agaaagtcat gcatacagtt tctgaaaatc agtgcggtaa
    12181 atacgcatta ctgctgcaac aggccagaac cgaagcacag gccgacgcag cgacgcgctt
    12241 ttcttctcat cttgacgcca tgattcgcca tatcacaaag gcggagttat cccgcgtgga
    12301 gatagtcgag ctgctcagtc aggagtcgga aaaatttcac aatatcgggt tgtctcgcgg
    12361 ggaggtgctt tgatgtcctg ttctcattca gttgtattac tgaacaatgc cttaaaaatc
    12421 gccgttatga aaaatggcga tttgtctctt attcaacttg gtcttgataa agaaaaacgc
    12481 gaaataactg aatctgttat cgcgatttat cagaacgaat taaacctcct gtctgatgtg
    12541 gtcaatttac ttgttaaacg cgctgtattt cataagcaaa tctcctccgt ggatgaactg
    12601 acgaaattaa cgacagaaat cgccagctat tgcgctgatg aatttaagaa gctgaacgac
    12661 aaaaggagct ggtaatgccg gacaacgtgg attttattca ggaacagcag gctgaattac
    12721 tggagcgcca gattaacgcg gcaagggtaa aacattgcgg tgcttctgcg ctggtttgtg
    12781 aagagtgtga cgtgccaata cctgctgccc gtcgtgcggc ttatccgtca gccacgcgtt
    12841 gtgtttcctg tcagtcagtc tttgaagcaa aaaacaaaca ttaccggaga atggcatgag
    12901 cattcgtata gaaattggcg aacgttatgt cgttaccagt gacagctttc agtttattct
    12961 ccatgagaaa aagagagcgg aaagcggtaa aaacgccggt caggagtggc tggcggtggt
    13021 tggttattac ccgaaattaa gccagctcgt ttccggcctg atgcatcacg atattctgac
    13081 cggaagcgca aagtcttttg ctgatttaaa cgcgcaggtt gagcaactca gcaagcgttg
    13141 ttcagaggct tttggctcat atggccatta aagcctccgg gcgttttgtc cctccgtcag
    13201 catttgctgc aggcaccggt aagacgttta ccggtgctta tgcatggagc gcgccacgcg
    13261 aggctgtcgg gcgcgaaaga ccccttacac gtgacgagat gcgtcaggtg caaggtgttt
    13321 tatccacgat taaccgcctg ccttactttt tgcgttcgct gtttacttca cgctatgact
    13381 acatccggcg caataaaagc ccggtgcacg ggttttattt cctcacatcc acttttcagc
    13441 gccgtttatg gccgcgcatt gagcgtgtga atcagcgcca tgaaatgaac accgacgcgt
    13501 cgttactgtt tctggcagag cgtgaccact atgcgcgcct gccgggaatg aatgacaagg
    13561 agctgaaaaa gtttgctgcc cgtatctcat cgcagctttt catgatgtat gaggaactca
    13621 gcgatgcctg ggtggatgcg catggcgaaa aagaatcgct gtttacggat gaggcgcagg
    13681 ctcacctcta tggtcatgtt gctggcgctg cacgtgcttt caatatatcc cctctctact
    13741 ggaaaaaata ccgtaaaggg cagatgacca cgaggcaggc atattctgcc attgcccgtc
    13801 tgtttaacga tgagtggtgg actcatcagc ttaaaggcca gcgtatgcgc tggcatgagg
    13861 cgttactgat tgctgtcggg gaggtcaata aagaccgttc tccttatgcc agtaaacatg
    13921 ccattcgtga tgtgcgtgca cgccgccagg caaatctgga atttcttaaa tcgtgtgatc
    13981 tcgaaaacag ggaaaccggc gagcgcatcg accttatcag taaggtgatg ggcagtattt
    14041 ctaatcctga aattcgccgg atggagctga tgaacaccat tgccggtatt gagcgttacg
    14101 ccgcagcaga gggtgatgtg gggatgttta tcacgctgac cgcgccgtca aagtatcacc
    14161 cgacacgtca ggtcagaaaa ggcgaaagta aaaccgtcca gctaaatcac ggctggaacg
    14221 atgaggcatt taatccaaag gatgcgcagc gttatctctg ccgtatctgg agcctgatgc
    14281 gcacggcatt caaggataat gatttacagg tctacggttt gcgtgtcgtc gagccacacc
    14341 acgacggaac gccgcactgg catatgatgc ttttttgtaa tccgcgccag cgtaaccaga
    14401 ttatcgaaat catgcgtcgc tatgcgctca aagaggatgg cgacgaaaga ggagccgcgc
    14461 gaaaccgttt tcaggcaaaa caccttaacc ggggcggtgc tgcggggtat atcgcgaaat
    14521 acatctcaaa aaacatcgac ggctatgcac tggatggtca gctcgataac gataccggca
    14581 gacctctgaa agatactgcc gcggctgtta ctgcatgggc gtcaacgtgg cgcatcccgc
    14641 aatttaaaac ggttggactg ccgacaatgg gggcttaccg tgaactacgc aaattgcctc
    14701 gcggcgtcag tattgctgat gagtttgacg aacgcgtcga ggctgctcgc gctgccgcag
    14761 acagtggtga ttttgcgttg tatatcagcg cgcagggtgg ggcaaatgtc ccgcgcgatt
    14821 gtcagactgt cagggttgcc cgtagcccgt cggatgacgt taacgagtac gaggaagaag
    14881 tcgagagagt ggtcggcatt tacgcgccgc atctcggcgc gcgtcatatt catatcacca
    14941 gaacgacgga ctggcgcatt gtgccgaaag ttccggtcgt tgagcctttg actttaaaaa
    15001 gcggcatcgc cgcgcctcgg agtcctgtca ataactgtgg aaagctcact ggtggtgata
    15061 cttcgttacc ggctcccaca ccttctgagc acgccgcagc agtgcttaat ctggttgatg
    15121 acggtgttat cgaatggagt gacccagagg tcgtcagagt gtttagaaac acattaaagc
    15181 attatttgcc aatgtttact cggcaagaga agataagtgg ctagctgata taattgcata
    15241 ggattacatt gttcgtttgg atggtcggag cttggagaga gtaactgtcg ttcacttgct
    15301 ttgagttttt caaaatgacc atcagcagca aattgggaac ttcagggggc tggagcgtgg
    15361 ctattaatca tccgaaaaat cactttctca cattaaggga ttgttagttt gattcaactt
    15421 ttaaagcaac tatggcttta gacaatgact gatgtcatga tagaatacat gatcgttagt
    15481 tgaatcttta gcatggttat ttgaatatga atgtagttga tttattctct ggagttggcg
    15541 gtttaagctt aggggcaact cgagccggtt tcaacttggt tggagctgtt gaactggata
    15601 aacatgctat tttttcacat gagttaaact tcccaaaatc aacacatttg catcgggatg
    15661 tctgctcgct tacagcaaaa gatattttgt ctgcatgtgc tgtaaaggac attgattgcg
    15721 ttattggtgg tccaccttgc caaggattta gcaccattgg aaaaaggaat gccaatgata
    15781 caagaaatga attgtatatt cattttttta ggttggtaag tgagttatct ccaatttgtt
    15841 ttttggcaga gaatgttcca ggaattatgg gtgagaaata taatccagta agagaagaag
    15901 cattttcttt agttagagat ggctattgtt tattacatcc aataagggtt aatgcttcaa
    15961 attatggagc acctacgaca cggacacgaa tatttttcat gggttttaaa agaggtttta
    16021 ttaatcagtt aaaagaatct gatttttttc ctaaaaatat aatagagcag actcttgtca
    16081 aagatgcact ctacggatta ccgcgaacta taagaaaaga gtggcagaca gaggagcaag
    16141 gctggcgttc agtaaaaatg gataaagatg gttactatta tgagcgactt tggggacata
    16201 taccaaatgg tgtaggtgat gcagaatcca ttcaaaattt aaagaaagga ttggtatctg
    16261 gctttttagg tacagctcat tcggaggaag tgctcaggag atatagacaa ctttcattcg
    16321 gtgaaactga taagatttcg agatcacaac gactcaatcc taacggtttt tgtccaactt
    16381 tgagagcagg tacaggaagt gacaagggga gttatcaggc cgttaggcca attcacccta
    16441 ctcaagctag ggtgataact cctcgtgaag cagctaggtt acaagggttt cctgattggt
    16501 tccgttttca tcctacaaag tggcacagtt ttagacagat aggtaatagc gttagtccgc
    16561 ttgtggctga ggctatgcta ttacctttat acgattactg tttaaaagtt aaaaaagaat
    16621 ctttgaatac catggaacga ccttgtagta ttcgataaaa ttacttagat cggagttggc
    16681 tccagaagga gtggagccaa tgtaaaaccc tagagtttca tggatgtttt ctactgaagc
    16741 ttcgggtaat aaagattcaa tatgtatcct ttcttttaaa gacatagcag ggacgtttat
    16801 atttttagga ttactccact tcccatcata aaatggccaa ttcttgattc tggaattatc
    16861 aataaattta gcttcttctg tatcagtgtt ttcaagaacg atagctgtta aagtaaccat
    16921 ctgttggcca tctttataaa cgaaagaagt taatggatgt attcttatgt catttcgacc
    16981 ttttaatcca tgccctattg cgcgtctcat agcattaact aggagcatag ggtatttttt
    17041 aaatccgaca tcttcgacta ctgcattcgg tggtaaaaaa tcttgagtca aaatatcgtt
    17101 tatcttttga aatctatatg ctcttggatc tcgttcgctc gggttttttc ccaaggcttc
    17161 agggtttgca tttaaagtga tcttaaaaat gtctttgggt tttagctttg taaccaaatt
    17221 aactatttca tttaactgag catttaagtc tgaaggcgtt gcataatcta accatatgat
    17281 cgttttatcg tcaaagttat agcgattgat gaagtctccg ctcatttctg gggtatcacc
    17341 caaatttatg caagataatg gtttgttaaa ctgttgtcgg caatggacat ttttatctcc
    17401 ttcgatagat atcattttag tcacttttaa caaattatga atttgtttga aatcttcgag
    17461 gaaaggcccc ccgaatccta tatatacata ttcggatatg tttgtataat tgtttaacct
    17521 tcttagggat tctataaata gattcctttc aatggctttg ttttgccgca aatgatatgg
    17581 gatgcttccg ccagtactca ttgtttagcc ttttttagaa attcatcaaa acaatattga
    17641 ccaacctctg ttggtggttg agttgcatca tcaaagagaa actctgacac cagttttatt
    17701 tcactatgtc tacggctaaa ttttatctga cgtaatggat ccgaatcccg aggcattggt
    17761 agcttcggtt tgaaaacttc tcctcctatt gaacgtcgaa cgttcgacca gttttctcta
    17821 ggaattgatt tagtaaagtc gacctcagtt gttgaaacca tattggatgc agtattaata
    17881 atttgctttc tttcctctgc gttacttttc catttgttgg taaagtccgt gaagaatttt
    17941 aaaccctcgc gcataaaatc tttagtagaa agatatagct cagagttacc atcaacacct
    18001 cgtttagttg tagtgatagg tagtaattca gcatttgatg atgtgaaaat aacaacacca
    18061 gaaattccga tgaattgagt atgatattgt ggaactccag cttctcccca acctgtaagc
    18121 ttggttttat ccgcatgtag gactacacga tcattgcata taattgtcca accagctttt
    18181 tctgttgtgg aacggcctga taataaattc tcttcttctt cctcactagg caagttacga
    18241 taaaatccaa ctgcaacttt aattgaaact ccatttgaat tatttttata tacataagga
    18301 gcaatgccgt cggtattgcc tttaatggct tcttcatcaa atattaaatt ggtgctattt
    18361 ggcttaacaa tacatccatt tactttaacc tcaaaccctt ttttaatgat taaactataa
    18421 tgatttgcta tgatattaat caaaacttct tcaaagccac gatcctttga tagggatgct
    18481 ttaatatctt tccttaaatt gttaatttgg atgctgacgc ctgtttcgtt taagtcaaca
    18541 ttatttcgct ctaactccaa agaccagttt tcatcatcag ccatccattc aggagaaatg
    18601 gtaactgaaa attgttcgtt ttcagttttg cttaaaatct gagcagatgt ccccattttg
    18661 aaaatcgctc gtttcattcc gataccatat acaccaattg tcgggagatc atccgcctca
    18721 cgttctgcag gtctacccaa cctaaacgca tagctttccg caagttttgc tggaatacct
    18781 ccgcaattat cagttattct gaaactattc tcatcaaatt caatatgagc atagtaaccc
    18841 ttgtaaacgt ctgatttacc gaaggtttgc tctggagtga aattgttttt tcttaataca
    18901 ccatctaggc agttatctag taaatctaga attgagtcag acaactcaat atctcgggtg
    18961 agcatctcaa caaagaatct ttttgctgga aatgctgcta ttttgacgtt tgacgaaacc
    19021 ataaattatc ctttttcgaa cagtaatttt cgcattcatg cataatggct gtgaacgaag
    19081 attcgtcttg attaggcgtg catgattttg catacaaaat gtatgaaaaa aaaagctgcc
    19141 tgtctatgac tgatagccgt ctagttactt ggttttcaag acaaatgcga ctgcatcaaa
    19201 accgtctcat gaagcgggcg ggcgaggcgg ggaaagcact gcgcgctggc ggtggtgctg
    19261 attttatttt ttcagcgtct gagcgcgtcg tgatggcgtt tagattgtgc gtcggggcgt
    19321 tggtgtgtct aaggggtgtt ttgtgcggtg gtgagcgtgt gagggcgtga tgacgggatg
    19381 taaaaaagcc gcccgcaggc ggcgatgttc agtcgttgtc agtgtccagt gagtagtttt
    19441 taaagcggat gacctcctga ccgagccagc cgtttatctc gcggatcctg tcctgtagcg
    19501 ggataagctc attgcggaca aagacctttg ccactttctc aatatcaccc agcgacccga
    19561 cgttctccgg cttgccgccc atcaactgaa aggggatgcg gtgcgcgtcc agcaggtctg
    19621 cggcgctggc ttttttgata ttaaaaaaat cgtccttcgt cgccacttca ctgagcggga
    19681 taattttaat gccgtcggct ttcccctgcg gggcatagag aaacagattt ttaaagttat
    19741 tgcggccttt cgacttaacc atgttttcgc gaagcatttc gatatcgttg cgatcctgca
    19801 cggcatcggt gacgtacatg atgtatccgg catgtgcgcc attttcgtaa tacttgcggc
    19861 ggaacagcgt ggccgactca ttcagccagg cagagttaag ggcgctgaga tattccggca
    19921 ggccgtacag ctcctgatta atatccggct ccagcaggtg aaacacggag ccgggcgtga
    19981 aggctgtcgg ctcgttgaag gacggcaccc accagtaaac atcctcctcc acgccacggc
    20041 gggtatattt tgccggtgag gtttccagtc tgatgacctt accggtggtg ctgtaacgct
    20101 tttccagaaa cgcattaccg aacaccagaa aatccagcac aaagcggctg aaatcctgct
    20161 gggaaagcca cggatgcggg ataaatgtcg aggccagaat attgcgtttg acgtaaatcg
    20221 gcgagctgtg atgcacggca gcacgcaggc ttttcgccag accggtaaag ctgaccggcg
    20281 gctcatacca tctgccgtta ctgatgcact cgacgtaatc cagaatgtca cggcggtcga
    20341 gtaccggcac cggctcacca aaggtgaatg cctccatttt cggggcgctg gcggtcattt
    20401 tttttgccgc aggttgcggt gttttccctt ttttcttgct catcagtaaa actccagaat
    20461 ggtggatgtc agcggggtgc tgataccggc ggtgagtggc tcatttaaca gggcgtgcat
    20521 ggtcgcccag gcgaggtcgg cgtggctggc ttcctcgctg cggctggcct cataggtggc
    20581 gctgcgtccg ctgctggtca tggtcttgcg gatagccata aacgagctgg tgatgtcggt
    20641 ggcgctgacg tcatattcca gacagccacg acggataacg tcttttgcct tgagcaccat
    20701 tgcggttttc atttccggcg tgtagcggat atcgcgcgcg gcgggataga acgagcgcac
    20761 gagctggaac acgccgacac cgaggccggt ggcatcaata ccgatgtatt cgacgttata
    20821 tttttcggtg agtttgcgga tggattccgc ctgggtggca aagtccatac ctttccactg
    20881 gtgacgctca agtattctga atttgccacc ggccaccacc ggcggtgcca gtaccacgca
    20941 tccggcgctg tcgccacggt gtgacgggtc gtaaccaatc cataccggtc gggagccgaa
    21001 cggatttgcg gcaaacggcg catagtcttc ccattcttcc agcgtgtcga ccatgcagcg
    21061 ttgcagctcc tcgaacggga ataccgatgc cttgtcatca acaaattcac acatgaacag
    21121 gtttttaaaa tcgtcggcgc tgttttcgcg tttaagctgc tcaatgtcga acaatgtgca
    21181 gccacctttc agggcgtcct caatggtgac aatctgccgc cactggccgt ccgcacagag
    21241 aagcccaccg gcaagggcgt tatgactgac gtcgatttcc acgcgttcgg cggcgctggc
    21301 gcgtccccgg ttgaacagtt cacctgacca gaacgggtag gcgtcgtgcg ccagcgtgga
    21361 cggggtggag aaataggtcg agcgcaggtg actctgtgag gccatacctg atgccacctt
    21421 acgcagtacc tgaaaattcg ggatccagaa aatctcgtcg acgtacaggt cgccgttatg
    21481 gctctgtgcg gtgttggagt tggtgccgag aaaaatcagt tttgcgccgt tattgcccag
    21541 gacaatcggg tcaccggtca ggtcaacgtc aaccagacgg gcaaaggcga tgatgtattc
    21601 gcggaacaca tacgcctgcg ttttactggc cgacagaaaa atctggttat gaccggtttt
    21661 cagggcgcgc agcagcgcct cgcgggaaaa ataaaacgtc gcgccaatct ggcgggattt
    21721 caggatatcg cggatgcggt gctcaagccc ggcgcgatac cagtgcaact gatattcgaa
    21781 agactgctca aagaaaatct gctccagctt ttcgatggcc tcgtcactga aaaaattctt
    21841 tttcggtttg cgacgcccgc ctttgttgcg gttagcgatg ttcggattaa ggtctgcctc
    21901 gttgccggtc tggctgtagc ggtttacccg tgccagtcgt tcaatctggc gtccgagcaa
    21961 gtcaatttcc ttgaagtcac cgcctgtttt ctgcggtttg atgatgagct gggtcagccg
    22021 cgcttccaga ctcatttcga cacggctgat gggggcaaca ctgtcccagc cgtcgcgctg
    22081 tttccagctc tgcaccgtcg ggcgtttcat ctgcaacatg gcggcaatct gcggcacgga
    22141 aaacccctgc cagtacagca gcgccgcctg acgacgcggg tcgtgtaaaa gagtggtgtc
    22201 tgtggtgatg gtcatgaata cctcgccgtg atgaatacac ggcaaggcta ctgagtcgcg
    22261 ccctgcgatt cgctaaggtg ctgttgtgtc agtgataagc catccgggac tgatggcgga
    22321 ggatgcgcat cgtcgggaaa ctgatgccga catgtgactc ctctaatcac tattcaggac
    22381 tcctgacaat ggcaaaaaaa gtctcaaaat tctttcgtat cggcgttgag ggtgacacct
    22441 gtgacgggcg tgtcatcagt gcgcaggata ttcaggaaat ggccgaaacc tttgacccgc
    22501 gagtctatgg ttgccgcatt aacctggaac atctgcgcgg catcctgcct gacggcattt
    22561 ttaaacgtta tggcgatgtg gccgaactga aggccgaaaa gattgacgat gattcggcgc
    22621 tgaaaggcaa atgggcgctg tttgcgaaaa tcaccccgac cgatgacctt atcgcgatga
    22681 acaaggccgc acagaaggtc tacacctcaa tggaaattca gccgaacttt gccaacaccg
    22741 gcaaatgtta tctggtgggg ctggccgtca ccgatgaccc ggcaagcctc ggcacggaat
    22801 acctggaatt ctgccgcacg gcaaaacaca accccctgaa ccgcttcaaa ttaagccctg
    22861 aaaacctgat ttcagtggca acgcccgttg agctggaatt tgaagacctg cctgaaaccg
    22921 tgttcaccgc cctgaccgaa aaggtgaaat ccatttttgg ccgcaaacag gccagcgatg
    22981 acgcccgtct gaatgacgtg catgaagcgg tgaccgctgt tgctgaacat gtgcaggaaa
    23041 aactgagcgc cactgagcag cgcctcgctg aggtggaaac cgccttttcc gcacttaagc
    23101 aggaggtgac tgacaaggtg gatgaaacca gccaggcatt cacccgcctg aaaaacagcc
    23161 tcgaccacac cgaaagtctg acccagcagc gccgcagcaa ggccaccggt ggtggcggtg
    23221 acgccctgat gacgaactgc tgaccggcgt cagtcagtcc gggaaaacct tcacgattaa
    23281 cccttaattt caggaaaaac tatgcgccag gaaacccgct ttaaatttaa tgcttacctg
    23341 tcccgtgttg ccgaactgaa cggcatcgac gccggtgatg tgtcgaaaaa gttcaccgtt
    23401 gaaccgtcgg tcacccagac cctgatgaac accatgcagg agtcctctga ctttctgacc
    23461 cgcatcaaca ttgtgccggt cagcgaaatg aaaggggaaa aaattggtat cggtatcacc
    23521 ggccccattg ccagcaccac cgacaccgca ggaggcaccg agcgtcagcc gaaggacttc
    23581 tcgaagctgg cgtccaacaa gtacgaatgc gaccaggtta acttcgattt ttatatccgc
    23641 tacaaaacgc tggacctgtg ggcgcgttat caggatttcc agctccgtat ccgtaacgcc
    23701 attatcaaac gccagtccct tgatttcatc atggccggtt ttaacggcgt gaagcgtgcc
    23761 gaaacctctg accgcagcag caatccgatg ctgcaggatg tggcggtcgg ctggctgcag
    23821 aaataccgca atgaagcccc ggcgcgcgtg atgagcaagg tcactgacga ggaaggtcgc
    23881 accacctctg aggtcatccg cgtgggtaag ggcggtgatt atgccagcct cgatgcactg
    23941 gtgatggatg cgaccaacaa cctgattgag ccgtggtatc aggaagaccc tgaccttgtg
    24001 gtgattgtgg ggcgtcagct actggcggac aagtatttcc ccatcgttaa caaggagcag
    24061 gacaacagcg aaatgctggc cgctgacgtc atcatcagcc agaaacgcat cggtaacctg
    24121 ccggcggtac gcgtcccgta cttcccggcg gatgcgatgc tcatcacgaa gctggaaaac
    24181 ctgtccatct actacatgga tgacagccat cgccgcgtga ttgaggagaa cccgaaactc
    24241 gaccgcgtgg agaactacga gtcaatgaac attgattacg tggtggaaga ctacgccgcc
    24301 ggttgtctgg tggaaaaaat caaggtcggt gacttctcca caccggccaa ggcgaccgca
    24361 gagccgggag cgtaaccgat gacgagtccc gcacagcgcc acatgatgcg ggtctcggca
    24421 gcgatgaccg cgcagcggga agccgccccg ctgcgacatg caactgtcta tgagcagatg
    24481 ctggttaagc tcgccgcaga ccagcgcaca ctgaaagcga tttattcaaa agagctgaag
    24541 gccgcgaaaa aacgcgaact gctgccgttc tggttgccgt gggtgaacgg cgtgctggag
    24601 cagggcaaag gtgcacagga tgacattctg atgacggtca tgctgtggcg tctggatacc
    24661 ggcgatattg ccggtgcgct ggagattgcc cgttatgccc tgaagtacgg tctgaccatg
    24721 ccgggtaaac accgccgcac cccgccgtac atgttcaccg aggaggtcgc gcttgcggcc
    24781 atgcgtgccc acgctgccgg tgagtctgtg gatacccgcc tgctgacgga gacccttgaa
    24841 ctgactgccg ctgctgacat gcctgatgaa gtgcgcgcaa agctgcacaa aatcaccggt
    24901 ctgtttctgc gtgacggtgg tgatgccgcc ggtgcgctgg cgcacctgca acgtgcgaca
    24961 cagctcgact gtcaggcagg cgtcaaaaaa gagattgaac gactggagcg ggagctgaaa
    25021 ccgaagccgg agccgcaacc caaagcggcc acccgtgcca cgcgtaagac ccggagcgtg
    25081 acaccggcaa aacgtggacg cccgaaaaag aaagccagtt aacaaccgaa tgcgccccgc
    25141 gccagggcgg cacgccggtc agtgagggtg aatcacctga cactacaccg gcgtccaccg
    25201 cccgactttt cagaggtagt catgatgacg ctgattattc cgcgaaagga ggctcctgta
    25261 tccggtgagg gtacggtggt catcccgcaa ccggcaggcg acgagccggt gattaaaaac
    25321 acgttctttt ttcccgatat cgacccgaag cgcgtccggg aacgtatgcg ccttgagcag
    25381 accgtcgccc ccgcccgtct gcgtgaggcc atcaagtcag gcatggctga aacgaatgcg
    25441 gagctgtacg agtaccgcga acagaaaatt gccgccggtt ttacgcgtct ggctgacgtc
    25501 ccggcagacg atatcgacgg tgaaagcatc aaagtttttt actacgagcg cgccgtgtgt
    25561 gcgatggcga ccgcgtcgct ttatgagcgt tatcgcggcg tggatgccag tgcgaaaggc
    25621 gacaagaagg ccgacagcat tgacagcacc attgatgagc tgtggcggga tatgcgctgg
    25681 gcggtggcgc gcatccaggg caagccgcgc tgcatcgtga gtcaaatctg atgaagacct
    25741 ttgcgctaca gggcgacacg ctcgacgcca tttgtgtccg ctattacggg cgcactgagg
    25801 gcgtggtcga ggccgtgctc gccgcaaatc cgggactggc tgaactgggg gcggtgctgc
    25861 cacacggcac cgccgtcgaa ctgcccgacg ttcagaccgc gcccgtggct gaaactgtca
    25921 atctgtggga gtaacgcatg acagcagaag aaaaaagcgt cctgtcgctt ttcatgattg
    25981 gggtgctgat tgttgtcggc aaggtgcttg ccggtggtga acctatcacc ccgcgtctgt
    26041 ttatcgggcg catgttgctc ggtggttttg tctcgatggt tgccggtgtt gttctggtgc
    26101 agtttcctga cctgtcactg cctgcggtgt gcggcatcgg ctccatgctg ggtatcgccg
    26161 gttatcaggt gattgagatt gccattcagc gccgttttaa gggcaggggg aaaccgtaat
    26221 gccggtaatt aacacgcatc agaatatcgc cgcctttctc gacatgctgg ccgtgtccga
    26281 agggacggcg aatcatccgc tgacgaaaaa ccggggctat gacgtgatag tcaccggact
    26341 ggacggaaag ccggaaattt tcaccgacta cagtgaccac ccgttcgcgc atggccgacc
    26401 ggcgaaggtg tttaaccgtc gcggtgaaaa atccacggcc tccggtcgct atcagcagct
    26461 ttacctgttc tggccgcact accgcaaaca gcttgccctg ccggatttca gtccgttgtc
    26521 acaggacagg ctcgccattc agttgatccg cgaacgcggt gcactggatg acatccgggc
    26581 gggacgcatt gagcgcgcca tttcacgctg tcgcaatatc tgggcgtccc tgccgggtgc
    26641 cggttacggt cagcgtgagc attcactgga aaaactggtc accgtctggc gtaccgccgg
    26701 cggcgtaccg gcttaaacgg agtaaacacc atgaagaaat tatccctttc actgatgctg
    26761 aacgtgtcgc tggcgctgat gctggcactg tccctgattt acccgcagag cgtggccgtc
    26821 agttttgtcg ccacctgggc gattctggcg acggttatct gtgtggttgc cggtggtgtc
    26881 ggcgtgtatg ccacggagta tgtgctggaa cgctacgggc gggagctgcc gccggaatcg
    26941 ctggccgtga agattgtcac gtcgctgttt ttgcagccgg tgccgtggcg cagacgggcg
    27001 gcggctctgg tggtgatggt ggcgacgttt atctcgctgg tcgctgccgg gtggattttt
    27061 accgcgctga tttatcttgt ggcgtcgctg tttttccggc tgatacgtaa agcctgtcgt
    27121 cagcgtcttg aggggcggga actatgtcaa agctgatgat tgtgctggtc gtgttgttat
    27181 cgctggcggt ggccggtctg tttctggtga aacacaaaaa tgccagcctg cgcgcctcgc
    27241 tggacagggc gaataacgtc gccagtgaac agcagacgac catcaccatg ctgaaaaatc
    27301 agcttcatgt tgccatcacc agggcagaca aaaacgagct ggcgcaggtg gcactgcgtc
    27361 aggaactgga gaacgccgcg aagcgtgaag cacagcgcga gaaaaccatc acgaggttac
    27421 tcaatgaaaa cgaagatttt cgccgctggt acggtgctga cctgcctgat gctgtgcgcc
    27481 ggttgcacca gcgcccggcc tgcgcagacg ccagtgattg tccacaacgc ctgcccgaaa
    27541 gtgagtcttt gcccgatgcc gggcagtgac ccgcagaaga acggcgattt aagtgccgat
    27601 atccggctgc ttgagaacgc gctggcacgc tgtgccagcc aggtaaaaat gattaaacac
    27661 tgtcaggacg aaaacgatgc tcaaacccga cagcctgcgc agggcgctga ctgatgccgt
    27721 cacggtgctg aaaaccagtc ccgagatgct gcggatattc gtggataacg ggagtattgc
    27781 ctccacgctg gcgacgtcgc tgtcattcga aaagcgttac acgctcaatg tcattgtgac
    27841 cgactttacc ggtgattttg acctgctcat cgtgccggtg ctggcgtggc tgcgggaaaa
    27901 tcagcccgac atcatgacca ccgacgaagg ccagaaaaag ggcttcacgt tttatgcgga
    27961 catcaacaat gacagcagct ttgatatcag catcagcctg atgctgaccg agcgcacgct
    28021 ggtcagtgag gtggacggcg cactgcatgt gaagaatatc ccggaacctc cgccgccgga
    28081 gccggtcact cgcccgatgg agctttatat caatggcgaa ctggtgagca agtgggatga
    28141 atgagtttaa gcgttttgaa gaccggctga ccgggctgat tgaatcactg tcaccatcag
    28201 ggcgtcggcg actgagcacc gaactggcga aacatctgcg gcagagtcag cagcgccggg
    28261 tgatggcaca gaaagccccg gacggcacac cctacgcacc acgccagcag cagagcgcca
    28321 gaaaaaagac cggtcgtgtt aagcgaaaaa tgtttgcgaa acttatcacc agtcgttttt
    28381 tgcatatccg cgccagcccg gaacaggcat caatggagtt ttacggcgga aagtcaccga
    28441 aaatcgccag tgtgcatcag ttcggtctgt cggaagaaac ccggaaagac ggtaagaaaa
    28501 ttgattatcc ggcgcgtcct ctgctcggct ttaccggtga ggatgtgcag atgattgaag
    28561 agattatcct ggctcacctt gagcgttagt tttatccagg cagaggctga tgcgcaatta
    28621 aacattgagc ggccatgctg gtcgctcaat gtttagaggt ttatgagtga tttttatttg
    28681 atgctttgta ttctaaaacc ttcttattgg cgtaaaagaa ttttgtatat gacaggaata
    28741 taaccagacc tgaagtgaaa tagacgaggg atagtattaa taatgctttt ttgtgactgt
    28801 tattgtcttt aatctcctgg cttaaccatt cggagtcctc ctcgtttaac tgtaagagct
    28861 tattgcaggc gatctcagga agtgtgtctt ttataaatac gttttgcagt ctcttgcaat
    28921 cggcaaggct ataagtttta ttaaattcaa ctgctttatt tttgaaggat aaaagaactt
    28981 tgtcactata aacatagtac atcatgtttt tatatggtat gcctatggca tcccttacta
    29041 taacggattg ttcgttgtgt atgtaacatg cgaggagaat gtaaaaaata ctggccagaa
    29101 ttacaattat tgttttaatt acgtgtggtg gttttgttat gtcaccccag atgcgagtaa
    29161 ggaaaaaata cgatgttttt agttttccat caatcagtcc ctgctgtatc attctcacat
    29221 tttcaatgcc tgatacattg attccgttaa ttattttaaa tagttgaatg tcgcgccact
    29281 cgcggtcaag tctttttaat tttttgtctg aatatccaaa attgagataa tgtgcaataa
    29341 gcctcataag gttactttta ccaaagctaa aaaatgctaa tactgcaaag ctacaaagga
    29401 aaaaaacgat tagcccccac acattagtca cattatagct gaccattacg ctctccttga
    29461 atgttgtctg gtagttctac aaatgaatcc agatagcata acttttatat attgtgcaat
    29521 ctcacatgca tgaacactct cgcaaatatt caggaactcg cgcgcgcact gcgcaacatg
    29581 attcgcaccg gcattatcgt cgaaaccgac cttaacgccg gtcgctgccg tgtgcagacc
    29641 ggcggcatgt gcaccgactg gcttcagtgg ctgacccatc gtgccggacg ttcacgcaca
    29701 tggtgggcac cttccgtggg ggaacaggtg ctgattctgg ccgtgggcgg tgaactcgac
    29761 acggcgttcg ttctgccggg gatttattcc ggcgataacc ctccgccgtc tgcgtcggcg
    29821 gatgccctgc acatacgttt ccctgacggg gcggtgattg aatatgaacc cgaaaccagt
    29881 gcactcacgg taagcggaat taaaacggcc agcgtgacgg cttctgattc tgttactgcc
    29941 acggtgccgg tggtcatggt gaaagcatca acccgcgtca ccctggacac cccggaggtg
    30001 gtctgcacca acaggctgat taccggcacg ctggaagtgc agaagggcgg gacgatgcgc
    30061 ggcaacattg aacacaccgg cggtgaactc tcatcaaacg gtaaggtact gcatacccat
    30121 aaacaccccg gcgacagcgg cggcacaacc gggagtcctt tatgacagcg cgttatctcg
    30181 gaatgaatcg cagtgatggc ctgactgtca ctgaccttga gcatatcagc cagagtatcg
    30241 gcgatatcct gcgcacaccg gtcggctcac gggtgatgcg tcgtgattac ggctcgttgc
    30301 tggcatcaat gattgaccag ccgcagaccc cggcgcttga gttgcagatt aaggtcgcct
    30361 gttacatggc ggtgctgaaa tgggaacccc gcgtcaccct gtcatccgtc accactgagc
    30421 gcagttttga cgggcgaatg acggtcacgt taaccggcca gcacaacgac accggccagc
    30481 cactttcatt aaccatccct gtgagttgaa accatgccga ttatcgacct gaaccagcta
    30541 cccgcaccag atgtggtcga ggagctggac tttgaaagca ttctcgctga acgcaaggcg
    30601 acactgattt ccctttaccc ggaagatcag caggaggcgg tcgcccgtac cctgacactg
    30661 gaatctgagc ctctcgtcaa actgctggaa gaaaatgctt atcgtgagct tatctggcgt
    30721 cagcgtgtga atgaggccgc acgggcggtg atgctggcct gtgccgccgg taatgacctt
    30781 gatgtgattg gtgccaatta caacaccacg cgcctgacta tcaccccggc agatgattcg
    30841 accattccac cgacaccggc agtgatggaa tctgacaccg attatcgtct gcgtattcag
    30901 caggcttttg agggcttaag cgtcgccggg tcagtgggag cctatcagta tcatggtcgc
    30961 agtgctgacg ggcgtgtcgc ggatatttct gtcaccagtc cgtctcctgc ctgtgtcacc
    31021 atctctgtgc tgtcacgtga aaataacggc gtcgcatccg aagacctgct ggctgtggtg
    31081 cgtaacgccc ttaatggcga ggacgtcagg ccggtggccg accgcgtgac cgtgcagtct
    31141 gccgccatcg ttgaatacca gataaacgcc acgctttacc tttaccctgg tcccgaaagc
    31201 gaacccatcc gcgctgccgc tgtgaaaaag ctggaagcgt atatcacggc acagcaccgg
    31261 ctggggcgcg acatccgtct gtctgccatt tatgccgctt tgcatgtgga aggtgtgcag
    31321 cgtgtcgaac tggctgcacc actggccgac atcgtgctca acagtacgca ggcgtctttc
    31381 tgtaccgaat accgcgtcgt gaccggaggc tcggatgagt gattcgcgac tgctgccgac
    31441 cggctcatca ccgcttgagg tcgccgccgc aaaagcctgt gcggaaattg aaaaaacgcc
    31501 ggtcagtatt cgtgagctgt ggaacccgga cacctgtccg gcaaatctgc tgccgtggct
    31561 ggcgtgggcg ttttcggtcg acaggtggga tgaaaagtgg ccggaagcga caaaacgcgc
    31621 cgttatccgc gatgcgtatt tcatccactg tcataaaggc acaataggtg caatccggcg
    31681 tgtggtggag ccgctcggct atctcatcaa cgtgacggag tggtgggaaa acagtgaccc
    31741 gcccggcacc ttccggcttg atattggtgt actggaaagc ggcatcacag aggcaatgta
    31801 tcaggaaatg gaacggctga ttgctgatgc caaacctgca agccgccacc ttattggtct
    31861 gaacattacc cgggacattc ccggctacct gtttgccggt ggtgtggctt atgacggcga
    31921 tgtaattacg gtttaccccg gataagtgag gaataatgag cacaaaattc aaaaccgtta
    31981 tcaccactgc cggtgcagca aagctggcag cggcaaccgc gccgggaggg cggaaggtca
    32041 acattaccac gatggccgtc ggggatggcg gtggtaaatt gcctgtcccg gatgccggac
    32101 agaccgggct tatccacgaa gtctggcgac atgcgctgaa caaaatcagc caggacaaac
    32161 gaaacagtaa ttatattatc gcagagctgg ttattccgcc ggaggtgggc ggtttctgga
    32221 tgcgtgagct tggcctgtac gatgatgcag gaacgttaat tgccgtggcg aacatggccg
    32281 aaagttataa gccagccctt gccgaaggct caggacgttc gcagacctgc cgcatggtca
    32341 ttatcgtcag cagtgtggcc tcagtggatc tgaccatcga caccacaacg gtgatggcga
    32401 cgcaggatta cgttgatgac aaaattgcag aacatgaaca gtcacgacgt caccctgacg
    32461 cctcgctgac cgcaaaaggt tttactcagt taagcagtgc gaccaacagc acgtctgaaa
    32521 cactggctgc aacgccgaaa gccgttaaga cggtaatgga tgaaacgaac aagaaagcgc
    32581 cattaaacag ccctgcactg accggaacgc caacgacgcc aactgcgcga cagggaacga
    32641 ataatactca gatcgcaaac acggctttcg ttatggccgc gattgccgcc cttgtagact
    32701 cgtcgcctga cgcactgaat acgctgaacg agctggcggc ggcgctgggc aatgacccga
    32761 attttgctac caccatgact aatgcgcttg cggggaagca accgaaagat gccaccctga
    32821 cggcactggc ggggcttgct actgcggcag acaggtttcc gtattttatg gggaatgatg
    32881 ttgccagcct ggcaaccctg acaaaagtcg ggcgggatat tctggctaaa tcgaccgttg
    32941 ccgccgttat cgaatatctc ggtttacagg aaacggtaaa ccgagccggg aacgccgtgc
    33001 aaaaaaatgg cgataccttg tccggtggac ttacttttga aaacgactca atccttgcct
    33061 ggattcgaaa tactgactgg gcgaagattg gatttaaaaa tgatgccgat ggtgacactg
    33121 attcattcat gtggtttgaa acgggggata acggcaatga atatttcaaa tggagaagcc
    33181 gccagagtac tacaacaaaa gacctgatga atcttaaatg ggatgctttg tatgttcttg
    33241 tcaatgccat tgtaaatggc gaagtcatat caaaatcagc aaacggccta cgtattgctt
    33301 atggtaatta cggattcttt attcgtaatg atggttcaaa tacatacttc atgttgacaa
    33361 actccggtga caacatgggg acttataacg gattaaggcc attatggatt aataacgcta
    33421 ctggcgctgt ttcgatgggg cgtggcctta atgtttcagg ggagacgctt tcagaccgtt
    33481 ttgctattaa cagcagtaat ggtatgtgga ttcagatgcg cgataacaac gctatctttg
    33541 ggaaaaatat agttaacact gatagcgctc aggcgttgct tcgccagaat cacgctgacc
    33601 gcaagttcat gataggtgga ctggggaaca agcaatttgg catctacatg attaataact
    33661 caaggacagc caatggcacc gatggtcagg cgtacatgga taataacggt aactggcttt
    33721 gtggctcgca agttattcct ggcaactatg gcaattttga ttccagatat gtgaaagatg
    33781 ttcgacttgg ttcacagcaa tattatggag tgaacaactg gcaaacatgg aatttccagt
    33841 gcccgtcagg tcatgtattg tctggtatta atgttcagga tacagggtcc aactctgccg
    33901 ataatatagc gggcgtttat tacagacccg ttcaaaagta tataaatggc acctggtata
    33961 atgtagcgag cgtttaatat gatgcactta aagaacataa aagcgggtaa cgctaaaaca
    34021 ctggaacagt atgagttaac aaagaaacac ggagttatct ggctttactc tgaggatgga
    34081 aaaaactggt atgaggaagt gaagaacttt cagccagaca caataaagat tgtttacgat
    34141 gaaaataata ttattgtcgc tatcaccaga gatgcttcaa cgcttaaccc tgaaggtttt
    34201 agcgttgttg aggttcctga tattacctcc aaccgacgtg ctgacgactc aggtaaatgg
    34261 atgtttaagg atggtgctgt ggttaaacgg atttatacgg cagatgaaca gcaacaacag
    34321 gcagaatcac aaaaggccgc attactttcc gaagcggaaa gcgttattca gccactggaa
    34381 cgcgctgtca ggctgaatat ggcgacggat gaggaacgtg cacgactgga gtcatgggaa
    34441 cgctacagcg ttctggtcag ccgtgtggat cctgtaaatc ctgaatggcc ggaaatgccg
    34501 caataagttg tataagctct ggtgtgagat tacatatcta tggcacagag taaagcctaa
    34561 tctgacaggc cgctctatgt ctggagtaga tttcagtaaa gcattatttt attagtgcaa
    34621 attctaatca atacatttta tgtatatgat atcctgcgga ctttaatgac ttggtttagg
    34681 ctaaccagat aacactgaag gaatattttt gataattaag gtgcggtatg tttactaagc
    34741 gacgatttaa aaatattaac tgggaggcaa gttcagtgat tcttgctatg gttctctttg
    34801 ttggaaatat attttataca aatcaccgtg atgatataag catggaggct gagagggaca
    34861 gtatcagaac aatgtttgca tatgaaatcg ctaataacca tcgcgctctc acttttcttg
    34921 ataaaacgag atatattggc tttgacgaaa attcggagca ttttgttggc gagccttttg
    34981 ccattaatgt caaatcatta ggggggcctc gcttacagat tgcattaaac cagactgata
    35041 aagtgtttaa atcctacttc agcgaattaa gtaagcttga taaagaggat gttactcttc
    35101 ttatggacta ttaccatgag caaagcatcc tgctggagcg tgtgaaatct acgttacaga
    35161 agatgaaaag tggtaatgat attaaagttg atattgatgg ttacttatta gaagaacact
    35221 tcatgaatga gcttaatctt tctaatattt tgcttaaacg ctatagccat ttgttgtcac
    35281 aatacgctaa agaacataaa acaaaagatt tacataattg ataatctgct aatggttatt
    35341 catggagtaa ctatgtttta tatgtcatcc aactcagatg aattattgat tctggataat
    35401 aaccacagag cggcatatac cctgacaggc aaatgtccgc ttcttgctca aagcagactg
    35461 tcagatttga taacttttgg gctatgtaaa ctgtcagtcg gaaaatgagt gtgtgcaaat
    35521 caggacaggc gggcgaattg cccgcatttt ctttatctgt tgtttcatcc actgaccagc
    35581 caggtcaaat ggcgtctcat gcactgccca acagaaaata gttgcaccca ttaaccacgg
    35641 agttaaacgg atgagcgact atcatcatgg cgtgcaggtg ctggagatta acgacggcac
    35701 ccgcgtcatt tccaccgtat ccaccgccat tgtcggcatg gtctgcacgg ccagcgatgc
    35761 ggatgcggaa atcttccccc tcaataaacc ggtgctgatt accaatgtgc agagcgcaat
    35821 tgcaaaggcc ggtaaaaaag gcacgctggc ggcgtcgttg caggccatcg ctgaccagtc
    35881 aaaaccggtc accgtagtcg tgcgtgtgga agacggcacc ggcgacgacg aggaaacgaa
    35941 actcgcgcag accgtttcca atatcatcgg caccaccgac gaaaacggtc agtacaccgg
    36001 actgaaagcc ctgctggcgg cggagtcggt aaccggtgtt aaaccgcgta ttctcggcgt
    36061 gccgggactg gataccaaag aggtggctgt tgcactggca tcagtctgtc agaagctgcg
    36121 cgctttcggg tatatcagcg catggggctg taaaaccatt tccgaggtga aagcctaccg
    36181 tcagaatttc agccagcgtg agctgatggt catctggccg gatttcctcg catgggatac
    36241 ggtcaccagt accaccgcca ccgcgtatgc caccgcccgt gcgctggggc tgcgcgctaa
    36301 aatcgaccag gagcagggct ggcataaaac gctgtccaat gtcggggtga acggtgttac
    36361 cggcatcagc gcctctgtat tctgggattt gcaggagtcc ggtactgatg ctgacctgct
    36421 taacgagtca ggcgtcacaa cgctgattcg ccgtgacggt ttccgcttct ggggtaaccg
    36481 tacctgctct gatgacccgc tgttcctctt tgaaaactac acccgcaccg cgcaggtgct
    36541 ggccgacacg atggctgagg cgcacatgtg ggcggtggac aagcccatca ctgcaacgct
    36601 gattcgcgac atcgttgacg gtattaatgc caaattccgt gagctgaaaa caaacggcta
    36661 tatcgtggat gcgacctgct ggttcagcga agaatccaac gatgcgcaaa ccctcaaggc
    36721 cggaaaactg tatatcgact acgactatac cccggtgcct cctcttgaaa acctgaccct
    36781 gcgccagcgt attaccgata aatacctggc aaatctggtc acctcggtta acagcaatta
    36841 aggagcctga ccgatggcaa tgccgcgcaa actcaagtta atgaacgtct ttctgaacgg
    36901 ctacagctat cagggcgttg caaagtccgt cacgctgcca aaactgaccc gtaagcttga
    36961 aaactatcgc ggtgcgggga tgaacggcag cgcaccggta gacctcggcc ttgatgacga
    37021 tgcgctgtca atggagtggt cgctcggggg cttcccggat tcggttatct gggagcttta
    37081 cgccgcaacc ggtgtggatg ccgtgccgat tcgttttgcc ggctcttacc agcgcgacga
    37141 taccggcgaa acggtggccg tcgaggtggt catgcgtggc cgtcagaaag aaatcgacac
    37201 cggcgagggg aaacagggag aagataccga gtcgaaaatc tccgtggtct gcacctattt
    37261 ccggctgacg atggacggta aggagctggt cgaaattgac accatcaaca tgattgagaa
    37321 ggtgaacggc gtcgaccggc tggagcaaca ccgccgcaat atcggcctgt gattttcatc
    37381 cggtcagcca ggctgaccgg ttaaccccga ttcagaagtg agaaaaccat gaacaaagaa
    37441 aatgtcatta ccctggacaa tccggtcaaa cgtggtgagc aggttatcga acaggtcacg
    37501 ctgatgaaac ccaatgccgg gacgctgcgc ggtgtcagtc tggcagcggt cgcgaactct
    37561 gaagtcgatg cactgattaa ggtgctgccg cgcatgacgg caccgatgct gaccgagcag
    37621 gaagtcgccg cgctggaact gcctgacctt gtggcgctgg ccggtaaggt ggtcggtttt
    37681 ttgtcgccga actcggtgca gtgacgtttc caaaaaatct gtcggtcgat gacctgatgg
    37741 cggatgtggc agtgatattt cactggccgc catcagaact gtatcccatg agcctgaccg
    37801 aactcatcac atggcgcgaa aaggcgctcc ggcgaagcgg aaacacgaat gagtaacaat
    37861 gtaaaattac aggtattgct cagggctgtt gaccaggcat cccgcccgtt taaatccatc
    37921 cgcacagcga gcaaatcgct gtcgggggat atccgggaaa cacaaaaatc actgcgcgag
    37981 ctgaacggtc aggcatcccg tattgaggga ttccgcaaga ccagtgcaca gctcgccgtg
    38041 actggtcatg cacttgaaaa ggctcggcag gaggccgaag cccttgccac acagtttaaa
    38101 aataccgaac gtccgacccg tgctcaggcg aaagtgctgg aatccgcaaa gcgtgcggcg
    38161 gaggacttac aggcgaaata taaccgcctg acggattccg ttaaacgcca gcagcgggaa
    38221 ctggccgctg tgggaattaa tacccgcaat cttgcacatg atgagcaggg actgaaaaac
    38281 cgtatcagtg aaaccaccgc acagcttaac cgtcagcgtg acgcactggc gcgtgtcagt
    38341 gcacaacagg caaaacttaa cgcagtaaaa cagcgttatc aggccggaaa ggaactggcc
    38401 ggaaatatgg cctcagtggg cgctgccggt gtggggattg cggcggcggg aacgatggcc
    38461 ggagttaagt tgctgatgcc cggttatgag tttgcgcaga aaaactcaga attgcaggct
    38521 gtgctcggtg tggcaaaaga ctccgctgaa atggctgcac tacgcaagca ggcgcgccag
    38581 ctcggcgaca ataccgccgc ctcggcggat gatgcggccg gtgcacagat aatcatcgcg
    38641 aaagcgggtg gggatgttga tgccattcag gcggcaacgc cggtcacgct gaatatggcg
    38701 ctggcgaacc gccgcacgat ggaagaaaac gccgccctgc tgatggggat gaaatccgcc
    38761 tttcagcttt caaacgataa ggtcgctcat atcggggatg ttctctccat gacgatgaac
    38821 aaaactgccg ccgattttga cggcatgagc gatgcgctga cctatgccgc acctgtggca
    38881 aaaaatgccg gtgtcagcat tgaagaaacc gccgcaatgg tcggggcgct gcatgatgca
    38941 aaaatcacag gctcaatggc ggggacggga agccgtgccg tgttaagccg cctgcaggca
    39001 ccgacgggaa aagcgtggga tgcactgaaa gagcttggtg tgaaaacctc agacagcaag
    39061 ggaaacaccc gaccagtatt taccattctg aaagaaatgc aggccagttt tgagaaaaac
    39121 cggctcggta ctgcccagca ggccgaatac atgaaaacca ttttcgggga ggaggccagc
    39181 tcagccgccg ctgtgctgat gactgccgcc tcaaccggaa agctggacaa actgaccgct
    39241 gcgtttaaag cctcagacgg gaagaccgcc gagctggtaa atatcatgca ggacaacctc
    39301 ggcggtgact ttaaggagtt tcagtccgct tatgaggcgg tggggactga cctgtttgac
    39361 cagcaggaag gcgcactgcg taagctcacg cagacggcca caaagtatgt gttaaaactc
    39421 gacggctgga tacagaaaaa caaatcactg gcgttaacca tcggcatcat tgtcggtggt
    39481 gcactggcac tgattggtgt catcggtgcc attggcctcg tagcctggcc ggttatcacc
    39541 ggcatcaatg ccatcatcgc ggcagcaggc gcaatgggag caatcttcac gacggttggc
    39601 agtgctgtta tgacggccat cggggcgatt agctggccgg ttgtggccgt ggtggccgcc
    39661 attgtcgccg gggcgttact tatccgtaaa tactgggagc ctgtcagcgc attctttggc
    39721 ggtgtggttg aagggctgaa agcggcattt gcgccggtgg gggaactgtt cacgccactt
    39781 aagccggtgt ttgactggct gggcgaaaag ttacaggccg cgtggcagtg gtttaaaaac
    39841 ctgattgccc cggtcaaagc cacccgggac accctgaacc gttgccgtga tacgggcgtc
    39901 atgttcgggc aggcactggc tgacgcgttg atgctgccgc ttaatgcgtt caacaaactg
    39961 cgcagcggta ttgactgggt actggaaaaa ctcggtgtta tcaacaaaga gtcaggcacg
    40021 cttgaccaga ccgccgccag aactcatgcc gccacgtatg gcaccggtgg ttatattccg
    40081 gcgaccagct cttatgcagg ctatcaggct tatcagccgg ttacggcacc ggctggccgc
    40141 tcttatgtgg accagagtaa aaacgaatat cacatcagtc tgacgggcgg tactgcgccg
    40201 gggactcagc tcgaccgcca gttacaggat gcgctcgaaa aatacgagcg ggataaacgt
    40261 gcgcgcgccc gtgccagcat gatgcatgac ggttaaggag gtgacgaaaa atgatgctcg
    40321 cgttaggtat gtttgttttt atgcgccaga cgctgccaca ccagaccatg cagcgtgaat
    40381 cagattatcg ctggccgtca aattcccgta tcggtaaacg ggatgccttt cagtttctcg
    40441 gtgtgggcga ggaaaacatc acgcttgccg gtgtgcttta tcccgaactg accggcggca
    40501 agctgacgat gaccacgctc agactgatgg cagaggaagg ccgggcgtgg ccgttgctgg
    40561 atggcaccgg catgatttac ggtatgtatg tcatcagcaa ggtgagtgaa acagggagta
    40621 ttttctttgc agacggcaca ccccggaaaa ttgattttac gctgtcgctc acccgcgttg
    40681 atgaatcact ggccgcgctt tatggcgata tcggtaaaca ggcggagtcg ctcatcggta
    40741 aggccggcag tctggcgacc agattcacag gtatgatggg ggcgggataa tgctggatgc
    40801 gctgacattt gatgcaggca gtacgctgac gccggattac atgctgatgc tcgacagcag
    40861 ggatattacc ggcaatatca gcgaccgtct gatgagcatg actctgacgg ataaccgggg
    40921 ctttgaagct gaccagcttg atattgaact gaacgatgcc gacgggcagg tcgggctgcc
    40981 ggttcgtggc gctgtcctga cggtgtatat cggctggaaa ggttttgccc tggtatgcaa
    41041 agggaaattc accgttgatg aggttgaaca ccggggcgcg ccggatgtgg tcaccatccg
    41101 cgcccggagt gcagatttcc gcgggacgct caattcccgc cgggaaggct cctggcatga
    41161 caccacgctc ggtgcgattg ttgaggcgat agcctcccgt aacaggctgg aagccagtgt
    41221 cgctccgtca ctggccggaa ttaaaatccc gcacatcgac cagtcgcagg agtctgatgc
    41281 gaaattcctg acccgtcttg cagaacgcaa cggcggtgag gtgtcggtaa aaatgggaaa
    41341 actgctgttt ctcaaagcgg ggcagggggt gacggccagc ggtaaaaaaa tcccgcagat
    41401 taccatcacc cgcagcgacg gcgaccgcca tcattttgcg attgctgacc gtggagccta
    41461 taccggcgta acggcaaagt ggttacacac caaagacccg aagccgcaaa agcagaaggt
    41521 aaaactgaaa cgcaaaaaga aagaaaaaca cctgcgcgca ctggagcacc cgaaagcaaa
    41581 accggtcagg cagaagaaag cgccaaaagt accggaagcg cgcgaaggtg aatacatggc
    41641 cggtgaggct gacaacgttt ttgccctgac cacggtatat gccacgaaag cacaggccat
    41701 gcgtgccgct caggcgaagt gggataaact gcaacggggc gttgcggagt tctccatcag
    41761 cctggctacc ggtcgtgctg atatttacac ggaaacaccg gttaaagtat caggctttaa
    41821 gcgcgtcata gacgagcagg actggacaat cactaaggtg acacattttc tgaataatag
    41881 cggcttcacg acgtccttag agcttgaggt caggctttct gatgtggagt acgaaacaga
    41941 agatgatgag tgatgtaatt tatttatctg tttgttttat aaggataaat taactaaaat
    42001 ggcaccatca acaaaaccgg aagaggtgct cgcgatgttt cattgtcctt tatgccagca
    42061 tgccgcacat gcgcgtacaa gccgctatat cactgacacg acaaaagagc gttatcacca
    42121 gtgtcagaac gtgaattgca gcgccacatt catcacttat gagtcggtac agcgatacat
    42181 cgtgaagccg ggagaagtcc acgccgtaag accgcacccg ttgccgtcag ggcagcaaat
    42241 tatgtggatg taattacaaa caggaagcc
//
LOCUS       DAPOZO010000041        42153 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPOZO010000041 DAPOZO010000000
VERSION     DAPOZO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.041"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(404..976)
                     /gene="hdeD"
                     /locus_tag="ROI21_002271"
     CDS             complement(404..976)
                     /gene="hdeD"
                     /locus_tag="ROI21_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDX5111186.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGVALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1231..1563
                     /gene="hdeA"
                     /locus_tag="ROI21_002272"
     CDS             1231..1563
                     /gene="hdeA"
                     /locus_tag="ROI21_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDX5111187.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            1679..2005
                     /gene="hdeB"
                     /locus_tag="ROI21_002273"
     CDS             1679..2005
                     /gene="hdeB"
                     /locus_tag="ROI21_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDX5111188.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2069..2716
                     /locus_tag="ROI21_002274"
     CDS             2069..2716
                     /locus_tag="ROI21_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDX5111189.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(2768..3538)
                     /locus_tag="ROI21_002275"
     CDS             complement(2768..3538)
                     /locus_tag="ROI21_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HDX5111190.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPASHSPLIVLDH"
     gene            complement(3535..4491)
                     /gene="chuU"
                     /locus_tag="ROI21_002276"
     CDS             complement(3535..4491)
                     /gene="chuU"
                     /locus_tag="ROI21_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ChuU"
                     /protein_id="HDX5111191.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(4576..5199)
                     /gene="chuY"
                     /locus_tag="ROI21_002277"
     CDS             complement(4576..5199)
                     /gene="chuY"
                     /locus_tag="ROI21_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HDX5111192.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVVRHADAATKLAQ
                     QGVQFFTGDACDASVVADACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(5199..5693)
                     /gene="hutX"
                     /locus_tag="ROI21_002278"
     CDS             complement(5199..5693)
                     /gene="hutX"
                     /locus_tag="ROI21_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDX5111193.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVKNLPSSTIVSG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKKGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(5706..7043)
                     /locus_tag="ROI21_002279"
     CDS             complement(5706..7043)
                     /locus_tag="ROI21_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDX5111194.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHHSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDDGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERDAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            complement(7063..7977)
                     /gene="shuT"
                     /locus_tag="ROI21_002280"
     CDS             complement(7063..7977)
                     /gene="shuT"
                     /locus_tag="ROI21_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HDX5111195.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKMFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLNSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            8661..10643
                     /gene="chuA"
                     /locus_tag="ROI21_002281"
                     /gene_synonym="shuA"
     CDS             8661..10643
                     /gene="chuA"
                     /locus_tag="ROI21_002281"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HDX5111196.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRGRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDTLEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            10692..11720
                     /gene="chuS"
                     /locus_tag="ROI21_002282"
     CDS             10692..11720
                     /gene="chuS"
                     /locus_tag="ROI21_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HDX5111197.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQMRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAETWVTRKPASDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(11781..12311)
                     /locus_tag="ROI21_002283"
     CDS             complement(11781..12311)
                     /locus_tag="ROI21_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX5111198.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12464..13030)
                     /gene="slp"
                     /locus_tag="ROI21_002284"
     CDS             complement(12464..13030)
                     /gene="slp"
                     /locus_tag="ROI21_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDX5111199.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(13275..14498)
                     /locus_tag="ROI21_002285"
     CDS             complement(13275..14498)
                     /locus_tag="ROI21_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111200.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNLEEQQ
                     PINSTFSGEGYTSDQYDLAQHQLQQINACHTNTTYTNAEYSKVVTQLVSLITNIETIS
                     STPLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQNLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIFSHKEHPAPKLYKDEQYLDKQIEG
                     LFSTLLTINDNFLRAKAEIASTIIKFLEARITNISYNDILKYQQEFKKQCYKQVKAFT
                     TLSRYNKIQTWAEMSEYQFEVFQRETLNPKKMSCAPYLKRSLPDEKTINYGVEIETPT
                     GKRIRLSNHYQNIIP"
     gene            complement(15079..16080)
                     /locus_tag="ROI21_002286"
     CDS             complement(15079..16080)
                     /locus_tag="ROI21_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDX5111201.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMALGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            16188..16484
                     /locus_tag="ROI21_002287"
     CDS             16188..16484
                     /locus_tag="ROI21_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX5111202.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(16607..17032)
                     /gene="arsC"
                     /locus_tag="ROI21_002288"
     CDS             complement(16607..17032)
                     /gene="arsC"
                     /locus_tag="ROI21_002288"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDX5111203.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIVYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQGAFTKEDGEKVVDEAGKRLK"
     gene            complement(17045..18334)
                     /gene="arsB"
                     /locus_tag="ROI21_002289"
     CDS             complement(17045..18334)
                     /gene="arsB"
                     /locus_tag="ROI21_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDX5111204.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFAVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLAENGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(18389..18742)
                     /gene="arsR"
                     /locus_tag="ROI21_002290"
     CDS             complement(18389..18742)
                     /gene="arsR"
                     /locus_tag="ROI21_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDX5111205.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSEDSKNICS"
     gene            19485..19568
                     /locus_tag="ROI21_002291"
     CDS             19485..19568
                     /locus_tag="ROI21_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDX5111206.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(19622..20974)
                     /gene="gorA"
                     /locus_tag="ROI21_002292"
     CDS             complement(19622..20974)
                     /gene="gorA"
                     /locus_tag="ROI21_002292"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDX5111207.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(21046..21888)
                     /gene="rlmJ"
                     /locus_tag="ROI21_002293"
     CDS             complement(21046..21888)
                     /gene="rlmJ"
                     /locus_tag="ROI21_002293"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDX5111208.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDNRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            22091..24133
                     /gene="prlC"
                     /locus_tag="ROI21_002294"
     CDS             22091..24133
                     /gene="prlC"
                     /locus_tag="ROI21_002294"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDX5111209.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            24141..24893
                     /gene="rsmJ"
                     /locus_tag="ROI21_002295"
     CDS             24141..24893
                     /gene="rsmJ"
                     /locus_tag="ROI21_002295"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDX5111210.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLLLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(24942..26411)
                     /gene="dtpB"
                     /locus_tag="ROI21_002296"
     CDS             complement(24942..26411)
                     /gene="dtpB"
                     /locus_tag="ROI21_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDX5111211.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(26727..27161)
                     /gene="uspA"
                     /locus_tag="ROI21_002297"
     CDS             complement(26727..27161)
                     /gene="uspA"
                     /locus_tag="ROI21_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDX5111212.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            27552..27887
                     /gene="uspB"
                     /locus_tag="ROI21_002298"
     CDS             27552..27887
                     /gene="uspB"
                     /locus_tag="ROI21_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDX5111213.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(27958..29457)
                     /gene="pitA"
                     /locus_tag="ROI21_002299"
     CDS             complement(27958..29457)
                     /gene="pitA"
                     /locus_tag="ROI21_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDX5111214.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            29689..30891
                     /locus_tag="ROI21_002300"
     CDS             29689..30891
                     /locus_tag="ROI21_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDX5111215.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(31206..32258)
                     /locus_tag="ROI21_002301"
     CDS             complement(31206..32258)
                     /locus_tag="ROI21_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDX5111216.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGIPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     SSKEHWLWCYWVIILGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            32641..34230
                     /locus_tag="ROI21_002302"
     CDS             32641..34230
                     /locus_tag="ROI21_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDX5111217.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKHRSKIVEID
                     KLDKHSQIDNDFGLHIIYFLQHGCWNVTEHSPQIEKLWFYNSEPSIDIKEYNSFADNT
                     TDTFIFTIIPDNNHVIKLSSPITVTIERKDGYYFINFSGDKSDIIYKVDGFSIIERNF
                     FTLLSGNFKPDWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYALLVSGHIGIRGEGWSLLCDLLKHEDMASFAYENKKLKKLFTLLDTRD
                     MILNELHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVVVLAGNHEINFNGNYSARLANHKLSSGDTYDLIKTLDVCNYDSETKVLTSHHGI
                     IRNEENESYCLGALQIPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRSNAIAST
                     PVYDNYFNNTTAFRPKPEDIFKCGQTLKKSKQKYGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQSH"
     gene            34492..36114
                     /locus_tag="ROI21_002303"
     CDS             34492..36114
                     /locus_tag="ROI21_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDX5111218.1"
                     /translation="MKLGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSFQDDKISFTIETNHSEKYSMLSGMRAHILRR
                     NNSYQFIVTINSKNYGCPLDDTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVSDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLGQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINKNVHVLAGNHETNC
                     NGNYMQNFTRMKPLGEDTYAGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHTIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNKNGLMPLIRRRTMLQL"
     gene            36480..37547
                     /locus_tag="ROI21_002304"
     CDS             36480..37547
                     /locus_tag="ROI21_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDX5111219.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            37544..40279
                     /gene="rbbA"
                     /locus_tag="ROI21_002305"
     CDS             37544..40279
                     /gene="rbbA"
                     /locus_tag="ROI21_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDX5111220.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPTRCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVIVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSSEAEAPPVVHDATHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            40279..41403
                     /locus_tag="ROI21_002306"
     CDS             40279..41403
                     /locus_tag="ROI21_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5111221.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(41602..41991)
                     /locus_tag="ROI21_002307"
                     /pseudo
     CDS             complement(41602..41991)
                     /locus_tag="ROI21_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT        11180 a         9904 c        10415 g        10654 t
ORIGIN      
        1 acctgagtaa ataacgcatt catgtcttca ggtttttata tagcgtctgg agacacggat
       61 atttatgcaa tgtaatcaac gcctgttgca aaaaaatgaa cccggattac ttatttaacc
      121 gcccatagca tcaacgctta cggaactctt tgttcacttt cccacagcac ttttaaaaca
      181 ttaaacctga ctacggaaaa tatcagccat gataatgttt gaaatggcta atttgccatg
      241 gaatgaaaaa aattagatga aattcagtag gttgaaataa tcatcagcag gtaattattt
      301 caatgatagt gcgcaattga tctacaacgc tgtgtagcgg agagagtatt aatcggatca
      361 tagtcacatc aagtgactat gatccgggtg acaacaagaa taattattgc tgcttaacga
      421 acaaactggc gaagctgaac aggctggcgg cgctaaatat cagttcaata cccaccagtg
      481 tggaaaccaa agttacagac accatcggcg ttgcaccaag gaatatccat gcaatgacga
      541 tatccagcac accaataacg agctgtagcc agctgccttt cattgaacgc tgacgatacc
      601 aactcatcag gcgaataacc cctgcaacac agaacaaacc ggcaataaat gccgcaatgg
      661 caaaaatgcc cagctccggt gcgcggatga agaaatagcc gatcaataaa taggcgactg
      721 cgacgaggaa accggataat accggccaga aattatgact gcggttgctg aataacccga
      781 caataagcgc aacgcccgag cagattaata atgcacccac taccgtgctt aaaatatcgc
      841 cagagacgaa cgggaaactg atacacagca acccgacgat aaacagcaac acggcaataa
      901 actggattgc tctgcgatgc tttttcagca tctccagatc aaacttcaaa attgttgcct
      961 tatctatata taacatagaa ccaccctata aaattaagaa gaaaatcccc tgctatcaat
     1021 ctatgccaaa aacgcgtcta agaatgcagt cgatttaata aaaatttcct aattacagta
     1081 tctgatgcat ctgtaactca ttgtattgaa ataaaaatat ctgattttga tattttccat
     1141 caacatgaca tatacagaaa accaggttat aacctcagtg tcgaaattga ttcgtgacgg
     1201 ctctttcact ttatagttga ggatattacg atgaaaaaag tattaggcgt tattcttggt
     1261 ggtctgcttc ttctgccagt tgtgagcaat gcagcggatg cgcaaaaagc agctgataac
     1321 aaaaaaccgg tcaactcctg gacctgtgaa gatttcctgg ctgtggacga atccttccag
     1381 ccaactgcag ttggttttgc tgaagcgctg aacaacaaag ataaaccaga agatgcggtt
     1441 ttagatgttc agggtattgc aaccgtaacc ccagctatcg ttcaggcttg tactcaggat
     1501 aaacaagcca actttaaaga taaagttaaa ggcgaatggg acaaaattaa gaaagatatg
     1561 taattccggg aatgcgttac atcgtacttc cttgcatatt gaacaggccg gaatatcttc
     1621 tttaaaagca gctattcctc ctgttcatat ataatctcta tattgaatgg gttacaaaat
     1681 gaatatttca tctctccgta aagcgtttat ttttatgggc gctgtagcgg ctttgtcact
     1741 ggtgaacgca caatctgcgc tggcagccaa tgaatccgct aaagatatga cctgccagga
     1801 atttattgat ctgaatccaa aagcaatgac accggttgca tggtggatgc tgcatgaaga
     1861 gacagtatat aaaggtggcg ataccgttac tttaaatgaa accgatctca ctcaaattcc
     1921 taaagtgatc gaatactgca agaaaaaccc gcagaaaaat ttgtatacct tcaaaaatca
     1981 agcatctaat gacttgccga attaatgagg tacaagtaaa aaggagtagc aagttgagcc
     2041 atcttgctgc tcctttttgc atttttatat gacagcagaa tttattatac gtcttatact
     2101 cgcagcaatt gcctgtggcg ctattggcat ggaaaggcaa atgcgcggca aaggagcagg
     2161 gttacgcaca catgtattaa ttggcatggg aagcgccctg tttatgattg tttcgaaata
     2221 tggttttgct gacgtgctgt ctttagatca cgtcggactg gaccccagcc gtattgctgc
     2281 tcaggtggtg acgggcgtcg ggtttatcgg cgcaggtaac attctcgttc gtaaccaaaa
     2341 tattgtaggt ctgacgacgg cagcggatat ctgggtgacc gccgctatag gtatggttat
     2401 tggcagtggt atgtacgaac tgggcattta tggttcagtg atgaccttgc tggtgctgga
     2461 agtcttccat caattaacct tccgcctgat gaacaaaaat taccatttac agctgacatt
     2521 agtgaacggt aatacggtat caatgctcga ctggtttaag cagcaaaaaa taaaaactga
     2581 tttagtttcg ttgcaggaaa atgaagatca tgaagtggtc gccattgata tccagcttca
     2641 tgccacaacg tcgatagaag acctgttgcg attattaaaa ggtatggcag gggtgaaagg
     2701 cgtttcaatt agctaatctt tatagttatt tattcacagg agtaccagag cggtactcct
     2761 gttggtttta atgatcgagg acgatcaggg gggaatggct ggcaggatct ttaagtacgg
     2821 taatatccgc gccgtaaagc atggtcagcg cctgctgtgt caatacgtcc tgtggtttac
     2881 catttgcgat aacctttcct ttttgcatta atacaacacg gtctgcataa cgcgcggcaa
     2941 gatttagatc gtgcaatacg cagcaaacat tgaactgccg ttcgtgaact agctggcgta
     3001 ataaacgaaa caagtgctgc tgatggtgaa tatccagcgc cgaagttggt tcatcaagaa
     3061 ataaccattt cggtgaggga gtaggttccc agagttgcac cagcaggcgc gctaattgca
     3121 cgcgctgttg ttcaccaccg gataattgcc gatagtcgcg gtttgctaac gcctgacagt
     3181 cacataacgc cattatttgt gctgtttcat tatcctgatt accagtgcga tgcggatgcc
     3241 gccccatctg gataacttct tgtacgctga atggaaacgc catgtggcta ttttgacgca
     3301 tcacggcccg gtgtttcgcc agttcagtaa tcgaccattc atttaatggc ttattaaaca
     3361 accgacattc gccgctatca ggttgaagat agccggttaa ctggcgaagc agcgtagatt
     3421 ttcccgcacc attagggccg agaatggcaa cgatttcccc accgggaaaa gtcagcgaga
     3481 cattatcagt cagacgccgc ccctgaaggc tataaaccag attctgggcg ctgatcatgt
     3541 gatcctccgg ttgcgcagaa tcaaccacat aaaataaggg ccaccaagca ggctggttaa
     3601 taatcccacg ggcatttctg ctggctgtac cagcgtgcgg gcaagcgtgt ctgccatcag
     3661 caataaacag gctccggcga gggcggagca agggattagc cagcggtgat ttgccccggt
     3721 agtcatacga atcagatgcg ggatcaccag gccaataaag ccgataatgc cgcttaccga
     3781 tacggcggca ccaacgagca gtgagctcac taacagtaat tgctggcgtt tacgcttaac
     3841 gttcacgccg aggtagtggg cttcttcatc accgagctgc agtaaattca gcgttccggc
     3901 gagacaagtt gttgcgataa tggccggtag gatgaatgaa ctggcaacca ataacgtcgg
     3961 ccattgcgcc tgtccaagat tgcccatcat ccacaatgtg agttgtcgta gctgctgctc
     4021 atcgccgata tagctcagga tgccgatcgc tgcgccgcac agggcattga tggcgatacc
     4081 ggcgagtaat aattgcatca tgctgccatc gcgatggcgc tgcgtgatga gaaaaatgat
     4141 ggtgcagacc actaaacttc cggcaatggc aaacaccatt tgctcataga gcaccagcac
     4201 cactgggaag gagaacggta gcacaatagc aacgccaacc attaatgcag aaccgctact
     4261 gacgccaagt aatcctgggt cagccattgg gttgcgaaaa agcccctgca taattacccc
     4321 tgcggttgcc aacgctgcac ctaccagcac cgccagcaaa acccgtggta gacgaatatt
     4381 gagccagata tcgcggtatt cctcatcaaa aagagcatca aaactgatct ttaacgctcc
     4441 ctgactggca ccgaacagca ccagagcgag caataatagt gatagcccca taaagaccct
     4501 ggcggaagag agtgaatcct tgagcacgca aaactccgtt acgacattac ggcgcggaga
     4561 ttccgcgccg ggggatcacg ccggtttcag gtccggttca atcagactgt agacctgctg
     4621 gttaagtgct ggcgcatttg ccagttcatg gatatgtgcg gcgacatcgg cacggttaac
     4681 aaaaccatga cactcctgat tttgtatccg ttgtgctttg cccgttgccg ccccatcaag
     4741 cagaccgcca gggcggagaa tggcgtaatc aagctggctg gtttgcaacc agctttccgc
     4801 cagcgttttt tcccgtaccg cctgaccaaa cgcagccttt gctcgctccg ataaaaacgg
     4861 ccagctatcg ccgcatccca aagaggtcac cagaatcatg cgactaatgc cggccttttc
     4921 ggcctcatca atcactgttc tgtgcgccag ataatcctga gctccgccca ttgttgagat
     4981 gataagcgca tccgggccag ctgcgcggca agcatccgcg actacgctgg catcacaggc
     5041 gtcgcctgta aaaaactgta cgccttgttg cgccagtttc gtggcggcat cggcatgacg
     5101 aacgacagca acgaccggac gttgctctgc cagcgccagt tcaagcgttc tggcacccac
     5161 accttttccg cctgcgccaa agagtagcca cggtgtcatc aggcgtgttc ctttaacgat
     5221 gctgccagag catggaaggc gctgacttgt tcgctcagga gctgacgatg gtcatccctg
     5281 ccgaggaaga ttttcagcat ggcgctgcct ttcttattga aaaagagaat agaggcggtg
     5341 tccatgccca taaatttacg ttcgatcaag gcaatatgcg tgcagttttc tgctttgata
     5401 tgcccggaca tgccgtgctt tccgcgtagg ttgaaatagc cgtggcggtg gaaacccgaa
     5461 ggcagttcgc cgctaaattc gaggataaca tcggcagtat gtactaacgt ggtgacgttg
     5521 ccccattcgc agacggtatc ccagacagta tcgaatttat cgcctgacac tatggttgag
     5581 gagggcaggt ttttcactac ttccagcagt gtggtgttgt attgttcggc taccacctcc
     5641 agcgtaccgt ccggctccgt tttcaaaaat tcttgtaacg agacatggct cataaatcac
     5701 tcctgttatg gtttttcacg cacaatcgct ggcgcattaa gtacctgaat tagttcatta
     5761 agagactgca aaatattgct tgcccagaaa cgaccttcat tagtcagccg caggcaagtt
     5821 gacgcatcgc ggcttaagcc tttttggtgc cattgagcca gtaacggcgc gagtttttcg
     5881 gcatgtggcg taagttcatc cagcggtaca cgcgctgttt caacacctga ctgcaaggta
     5941 tgacgccatt gcgcatcacg ttcggcgtta cgcatgatca tcatcagcgg ttttttgcct
     6001 gccgcgacgg attcatgcca ggtctgaaga ttgcgttcgt tcatccagga gtaaccatta
     6061 atcgaaccgc cggctccgga accaaaggcc agacaatcgg caccttgttt tatcagcagg
     6121 ttatagagat tgcgttcgcg tgtggtacgg ccccagtggc tgttactgat gcagcgccaa
     6181 ccgtcatcgt ccataaaatc acacccttgc agataaagat cgcgacgttc tgccggagag
     6241 ggcacggtag tgcgcccatt ttccacggct ttgcccagcg gtgtattggg caggacattg
     6301 agcgcataga gatcgacgcc gtcgagaccg atatcgcgag caatagccag atcttctccc
     6361 caggtttgcg catcctgacc cggcaggcca aacagcagat cacagaccac cgcagcacgg
     6421 tcgcgtttga ccaggctttc cataaacgca atggcggttg ggccatccga ggtgcgggcc
     6481 attttcttac ggattttgct gttaaacgac tgaataccaa ttgagaagcg gtttgctcct
     6541 gcatcaaggc aagcatcgat tcgctcagcg tcaaaattca gtacccggcc ttcgatagtg
     6601 atttcacaat caggggccag tggcagcttt tctcgtaatg tggtgatgat tctggccaaa
     6661 tcgtgtgccg aaagcgcgga aggcgtaccg ccaccgaaat agaccgcgtg gatgggggca
     6721 gaatgatgca atacgctgtc cgcttccatt tctatttcac gaatcagggc gtcggtataa
     6781 tgagcacacg catcttcatt aaaacggttc tgataaaaac cacaaaacgt gcagtgtgtc
     6841 gcacaaaaag gaatgtgtag ataaaccagt cgcttacgtg gtgataccgt ttgattgatc
     6901 atctcttgcc aggtctgtgc cagttgctct ttggcaaccg gaatggctcc acgaaacggc
     6961 atcatggcgc gcctgtcttt aaatggctga tcaccgtcta aagcaaaatg tggcgtgaga
     7021 tccagggtgt tatttgcgtt cattcgtctg atttaaatcc atttattgcg gccaaagctg
     7081 ctggtgtaaa gactcaacga catcagcaat acgcggaccc attcccagaa tcaggttttg
     7141 atcaacggtg ataatgcgct ggtttttcca tgcggcagtg tgggtaattc cggcaataga
     7201 acgtagccga ttaatatcgc catcgaccat ttgtgaggtt acgacaatca cttctggatt
     7261 ggccgcaatc agtgattctg cactgtagct tttgtactgc tggtgggttg cgacgttttc
     7321 tgctccggca agcgaaagaa tggcgtccgc gacgctgcct ttaccggcaa cctgtggcgc
     7381 acttccgcct gcggagagaa taaacatggc tttaaccggg gcttttttgg ccgccacgtt
     7441 ttgctgtact cgctccaggc gttggttgat ctgtgtcacc agcgcctcgc cttgttcagg
     7501 aacctgtagc gtttttgcca gttggcgaat gttggcgtac atctgctcaa gggtggcagg
     7561 cacacgcggc agggtaacga cgttgacttt ctgcgctcgt agttggtcga gcacaatttg
     7621 tggcccggca tcctgccagg taattacgct atccgggcga agtgacaaaa tgccttcgct
     7681 gttgagttgt ttccagtagc caatatgcgg cagtttggcg gtttctggtg gataagatgt
     7741 cgtttcatcg acaccaacca cgcgttcgcc agcgcccatc gcgtagatta gctccgtcag
     7801 cgatcctcct gcgaccacga tacgttcggc agccgttacg ctaaaagccc agctaagagc
     7861 cagtactgcc gtaaacattt tttttcgttt taccgcggtc ttcgcggcag aggccacggc
     7921 tgaaatacat aacgttagtg gggaaaaaag aaacggcctg gtgatgatcc ttggcataat
     7981 atgagaactc ttatcaaatt gagaatgatc attattatca tttgagttat atatgaaata
     8041 caaccgtcga ttcaatacgc aaggcgttac aagcgtattt atttttgatt tttatcaaga
     8101 tgcttcgtca gctaacgttc ttgttaccga ctaaatttca gcgcgggaaa aagctgagaa
     8161 cccacagata tcgaggcttg cagagcaaag cgaagagaaa attatcactg ctaacgacaa
     8221 agtttagtaa tgaggtcatg ggggaataac cataaaaaca aagtggttta cagggcgcag
     8281 gataagtttc ataaccccct gtttattgtg ttaatggcgg ttaaaaaaaa gcttgatcat
     8341 aaaaaattga tcgataataa ttctcattat cacgttcgcg cttaacaaaa cacgaccata
     8401 tggataacaa aaccatagca tttgcaaggg ataacgcaca gcgctcttca ctcctgatgg
     8461 ggcatatcgt tttgcctgac aaagagggcg tgcttttgcg tatttgccga gataccaggc
     8521 aatttcatgg gatataaacg cctgtaaact cacccggcga atattatcgc tctgaatttt
     8581 aaataaattt atttttcgcc agccaccccg gtattaaccc aggcagccag cattttttat
     8641 tttcctcatg gagaatcgct atgtcacgtc cgcaatttac ctcgttgcgt ttgagtttgt
     8701 tggctttggc tgtttctgcc accttgccaa cgtttgcttt tgctactgaa accatgaccg
     8761 ttacggcaac ggggaatgcc cgtagttcct tcgaagcgcc tatgatggtc agcgtcatcg
     8821 acacttccgc tcctgaaaat caaacggcta cttcagccac tgatttgctg cgtcatgttc
     8881 ctggaattac tcttgatggt accggacgaa ccaacggtca ggatgtaaat atgcgtggct
     8941 atgatcatcg cggcgtgctg gttcttgtcg atggtgttcg ccagggaacg gataccggac
     9001 acctgaatgg cacttttctc gatccggcgc tgatcaagcg tgttgagatt gttcgcggac
     9061 cttcagcatt actgtatggc agtggtgcgc tgggtggagt gatctcctac gatacggtcg
     9121 atgcaaaaga tttattgcag gaaggacaaa gcagtggttt tcgtgtcttt ggtactggcg
     9181 gcacggggga ccatagcctg ggattaggcg cgagcgcgtt tgggcgaact gaaaatctgg
     9241 atggtattgt ggcctggtcc agtcgcggtc ggggtgattt acgccagagc aatggtgaaa
     9301 ccgcgccgaa tgacgagtcc attaataaca tgctggcgaa agggacctgg caaattgatt
     9361 cagcccagtc tctgagcggt ttagtgcgtt actacaacaa cgacgcgcgt gaaccaaaaa
     9421 atccgcagac tgttgaggct tctgatagca gcaacccgat ggttgatcgt tcaacaattc
     9481 aacgcgatgc gcagctttct tataaactcg ccccgcaggg caacgactgg ttaaatgcag
     9541 atgcaaaaat ttactggtcg gaagtccgta ttaatgcgca aaacacgggg agttccggcg
     9601 agtatcgtga acagataaca aaaggagcca ggctggagaa ccgttccact ctctttgccg
     9661 acagtttcgc ttctcactta ctgacatatg gcggtgagta ttatcgtcag gaacaacatc
     9721 cgggcggcgc gacgacgggc ttcccgcaag caaaaatcga ttttagctcc ggctggctac
     9781 aggatgagat caccttacgc gatctgccga ttaccctgct tggcggaacc cgctatgaca
     9841 gttatcgcgg tagcagtgac ggttacaaag atgttgatgc cgacaaatgg tcatctcgtg
     9901 cggggatgac tatcaatccg actaactggc tgatgttatt tggctcatat gcccaggcat
     9961 tccgcgcccc gacgatgggc gaaatgtata acgattctaa gcacttctcg attggtcgct
    10021 tctataccaa ctattgggtg ccaaacccga acttacgtcc ggaaactaac gaaactcagg
    10081 agtacggttt tgggctgcgt tttgatgacc tgatgttgtc caatgatacc ctggaattta
    10141 aagccagcta ctttgatacc aaagcgaagg attacatctc cacgaccgtc gatttcgcgg
    10201 cggcgacaac tatgtcgtat aacgtcccga acgccaaaat ctggggctgg gatgtaatga
    10261 cgaaatatac cactgatctg tttagccttg atgtggccta taaccgtacc cgcggcaaag
    10321 acaccgatac tggggaatat atctccagca ttaacccgga taccgttacc agtaccctga
    10381 atattccgat cgctcacagt ggcttctctg ttgggtgggt tggtacgttt gccgatcgct
    10441 caacacatat cagcagcagt tacagcaaac aaccaggcta tggcgtgaat gatttctacg
    10501 tcagttatca aggacaacag gcgctcaaag gtatgaccac tactttggtg ttgggtaacg
    10561 ctttcgacaa agagtactgg tcgccacaag gcatcccaca ggatggtcgt aatggaaaaa
    10621 ttttcgtgag ttatcaatgg taatcatctg ccccgatatt tcggggcatt tatctggaag
    10681 gaagagagac aatgaaccac tacacacgct ggcttgagtt aaaagaacaa aatcccggaa
    10741 agtacgcgcg tgacatcgca ggtttaatga atattagcga agcagaactg gcatttgcac
    10801 gcgtcacgca cgatgcgtgg cggatgcgcg gcgatatccg tgaaattctg gcggcgctcg
    10861 aaagtgttgg cgagaccaaa tgtatttgcc gtaatgaata tgcagtccat gagcaagttg
    10921 gtgcgttcac aaaccagcat ttgaacggac atgccggatt gatcctcaat ccacgcgcgc
    10981 tggatttgcg tctgtttctc aatcaatggg ccagtgtttt tcacattaaa gaaaatacga
    11041 ctcgtggcga acgccagagt attcagttct ttgatcatca gggcgatgca ttactaaaag
    11101 tttatgccac cgacaatacc gatatggcgg catggagtga gcttctggca cggtttatca
    11161 ctgatgagaa tacgccgctt gagttaaaag ccgttgatgc gccagtcgtt caaatgcgag
    11221 ccgatgccag tgtggtcgag caagagtggc gggcgatgac cgacgttcat cagtttttta
    11281 cgttgctcaa gcgccacaac ctgacgcgcc aacaggcgtt caatctggtg gcagacgatt
    11341 tggcctgcaa agtatccaac agtgcgttgg cgcaaattct tgaatctgca cagcaggatg
    11401 gcaatgaaat catggtgttt gttggcaacc gtggctgcgt acagattttc actggcgtgg
    11461 tagaaaaagt ggtgccaatg aaaggttggc tgaatatttt taacccgacg tttactcttc
    11521 atctattaga agagagcatt gctgaaacct gggtaacccg taaacctgct agtgacggtt
    11581 atgtgaccag tctggaattg tttgcccatg atggtacgca gatagcgcaa ctttatggtc
    11641 aacgtacaga aggcgaacag gagcaagcgc aatggcgtaa gcaaattgct tcgctgatac
    11701 cggaaggcgt tactgcataa cgtataatta aagggaggtc tggctattta ttgctgggcc
    11761 tctttatatt aatcaatcga tcacaccaga taatcaatat gctgatggcg taccaactca
    11821 ttgatccgct taagattgag gatcatcatg atattgtgtt tatggcagta gaacgttttt
    11881 agcgatattt tcagtttctc cagaatatct tccgttgtca tttgattcgc aatgcagctc
    11941 agaacctgat gctgcttgcg cgaaagggac acatccgctt caggcacaga gcagtaactg
    12001 ccattgatga gtgcgacaaa gtcgatgatg gtgatagttc gcggaacaaa ggtcaccggt
    12061 actttcccca ggcagcgttt aatattaaag ggagagaaaa taatcacatg gacaggtttt
    12121 aatcgttcaa tctgagtgag caaattagaa tgaaatacat tattcattaa tgtatcaata
    12181 atgacgaagg catgctgatg caacattacg tcgatctctt cttcgttctg tatatgaacg
    12241 acattacctt tactcagaat gtttttcatc gcagtgaaga acatcgtatc cctggtaatt
    12301 ataagaaaca taaccgactc ctggtgcgga cattaacttt ataatcagtt tgccaaaggt
    12361 aattaacagg acagggctta taataatagt tagctggctg tgtattaatt tgatgtaatg
    12421 catgatgcag atgctgagaa agcatctgca tctttcggtg gtgttatttg accagctcag
    12481 gtgttacctg actcaccgcg ttggtgtagt aaggcgcacc ccagcccggt tccggccaga
    12541 atgcaccata gccgtaatcc cacaggttat aggtggtatt aaccacttct ctcaaatgcc
    12601 acacctggat gccctgcata ttcacttcga ggaagttata cgggacttta ttgataaagc
    12661 caggttgttc accctgaatg gtaccgagga tggtaacaaa gtgattacga tagttcactg
    12721 gatcaaggaa gccgctttgt ctggcgagca gtcggccctg atagttggct tcaatatcag
    12781 gcttcgcata gctatccaac ggtaatacag cgatttctaa caacgtatcc gttttgccat
    12841 tgataacgtt gataaccttc ccaccaaagc gcgcttgttg accaacatat aaccccggct
    12901 ggttatgaac agcaacaaaa cttttttgaa tatcaggttg gttattgcct ttgatatttt
    12961 gcggaattga actacatgcg gcaagcaaaa atgaaaggct gaggatgagt gcaccttttg
    13021 tcatgttcat gttactatcc ttatcaacaa atattaaaac cataatagat atttctttat
    13081 agttttcatc tgattctgag gcgatatgcc tgatagtaac gtaaacctgt ctgatgttta
    13141 tatcccttct gtttagttta attctgttaa ttttggggcg tgacatgagt atccatcgcc
    13201 ccataaaata gccttttttt aaaggtacat aaaatctatt tttattcaat gattataaag
    13261 tgctaatgtt catattatgg gattatattc tggtaatgat ttgataagcg aattcttttg
    13321 ccagttggtg tttctatttc caccccataa tttatagttt tttcatcagg tagcgatctc
    13381 tttaaataag gtgcgcatga catttttttt gggttaagcg tctcgcgttg aaatacttcg
    13441 aattgatatt cagacatttc tgcccatgtt tgaattttat tgtatcggga gagtgtcgta
    13501 aaggctttaa cttgcttata acattgtttt ttgaattctt gctgatattt taatatgtca
    13561 ttgtatgaaa tattggttat cctggcctct aagaatttaa ttattgtcga agcaatttcc
    13621 gcttttgctc gtaaaaagtt gtcattaatg gttaataatg ttgaaaataa accttcgatt
    13681 tgtttgtcaa gatattgttc gtctttatat aattttgggg cagggtgttc tttatgtgaa
    13741 aaaatagtgt cacctaaatt tgcgtcagtg aaatttatat taagtagatc ttcgaaataa
    13801 aaccttgaca aggttagaac gaacccggac aggttctgtc ctgtcatatt gaaatcaata
    13861 aagttggttc cggcaaagac tttcatcctg tttttatttt cactgtgctc aatatataac
    13921 ttcgtcattt tttgatacag gttttgaata tcttttatat tagtcggtaa gtcagctcgt
    13981 tgttcagcaa taaaattagt atctgaaaac atggtttgga cactgaatct tactggtatt
    14041 ccctcttcta cgctaatttt agttatgatt gccttgtcgg gtttaggatt agcgacaaca
    14101 atgattcgac attcagcact tttgtttttt tcgtatctaa tgttattaat ctgattaagt
    14161 attgattgtg tctgttgcgt aagtggtgtc gaacttatcg tttcaatgtt agtaataaga
    14221 cttacaagtt gagtcacaac ttttgagtat tctgcatttg tatatgtagt gttcgtgtgg
    14281 cacgcattaa tttgttgaag ttggtgttgc gctaaatcat attgatcaga ggtatatcct
    14341 tctccagaga atgtggaatt aatgggttgt tgttcttcca aattatcgca aggccacttc
    14401 ggtgtgtctg gcgttttagc attatcggta tttgttgtgc agttataaat atcgccgtga
    14461 tatgtattta ttatacctgg ggtaaagtca attgacataa aattccttat tcatcatttg
    14521 ttttatgcta agatgtttaa attggataat tagaacagga atggattttc ttctacacac
    14581 aataaatgta atgcgttatc tcttcctgat ataattacct ttgatttccc ttttatctcc
    14641 acccggtaat atttgtgtat aaaaaaaggg cagtgatagt atcgtaaagg aaaacgttaa
    14701 caactatcaa aaagcgctca ttttaaaaag ttatgatgtt caccgatagt gtgtgaagtg
    14761 atatttttgt gatgttttag atgtgtaata atgaaccagg ttaaagtgta tttaaaaatg
    14821 tgttgtattg gcgactgaaa tctgcccttt aaaagggggg atagcactga aacaaagaga
    14881 cattgcttct ccgataaaat gtcagagaag catgattgcg tggtgattgt cgattgatga
    14941 cttactgacg gatccgtcat cattctcaaa gcattagtta agttatttgt gatttttaaa
    15001 gattatccta tctatgattt tctactcaga aggataaggc actcacaatg ctccttatag
    15061 tttactgctg gcatctattt aaaatagcat ttgagtcaaa tagcccatca agatagccat
    15121 acctaatata accgtcagga atgcaactat catcggtatt ctgaacattg atttaagtaa
    15181 tatcacttcc gtcaggcttg cgccggcact gccgatgatt aatgccatta atgctcccag
    15241 agccattcct tttgtcatca gaacagatgc cagagggata accgcctctg cccggatata
    15301 tagcggaata ccgacaacgg cgctcaatgg gatggcgaag ggattatctg cccctgcatg
    15361 agcggcaatc cactctgagg gaataaagcc ataaataaaa gagcctatta atacccctaa
    15421 caaaagatat ggcagtacat ctttaaactg ttgcaacgca tctttggtgg ctagttttat
    15481 gggattaata cttaccacag ctttggtact gcaacagttt actacaggtt tctcaatggc
    15541 tttagccgct ggactacagc aactcacttt tattggcgta tatgtcgtcc ccggcgaagt
    15601 tttggctgga gcacaacaat ttgctgatga gcttttactg gcaataatat gacgttcaaa
    15661 tcccagagaa tccaggataa tactggcaag tacggagacg ccggcggcga taatcgcgta
    15721 caacaaggtc actttccagc caaaggtcac ccacattagt ccgacgataa tgggattaag
    15781 tagcggtgaa acaaataaaa aagtaagggt tggaccaaac ccggctttcg ctgacaacaa
    15841 tccacgtaac atgggtattg tcgagcaact acagaacggg gtaacggctc ccaacagagc
    15901 agccaggaga taacctctcc cttttctggc ccccatcatc tgctggattt tatggtctgg
    15961 caccttttgt cttatcaggc tcacaccggc actaatcact ataaacagca aagaaagctc
    16021 aacggcgaga aacacaaaca tctctgcggc atcttgcagc atcgcaagcc aactactcat
    16081 acgtaacctc attgaataaa tctatatttc cagaataatc gaaatgaaat tcagatcaag
    16141 atatttctgg tattatcgaa atatcaacca gacaaagggg gtgtcatatg cagttggaag
    16201 aagttgctaa agcgctgaaa gagctaggtc acccgacccg tttgttcata ttcaaacacc
    16261 tggtcaaagc gggggaacaa ggtttgccgg tcggtgagtt gcaaaagcag cttgggatac
    16321 ccggctctac actcagtcac catatttcag cactggtatc ggtagggctg gtcaaacaaa
    16381 accgtgagag tcgaacgctg atgtgtgttt cgcaatacgc aatgctggaa gcgataattg
    16441 aattcttacg cgaggagtgc tgcgtaaata gcaaaattgc ctgatgcgct acgcgctaca
    16501 gatttcatgc catgcacggt tttgtaggtc ggataagacg cgttagcgtc gcatccgaca
    16561 taaacaaagc gcactttacc aactatgggc agtggatatc gccgctttat ttcaagcgtt
    16621 tacccgcttc atcaaccact ttctcgccat cttccttggt gaacgcgcct tgttgcgcat
    16681 ctggcagaat ttccagcacc acttctgaag ggcgacacag gcgagttccc agcggcgtca
    16741 ccacaatcgg gcgattaatc agaatcgggt gctgaagcat aaagtcgatt aaccgatcgt
    16801 cagtaaattt atcttctgca aggcccagtt cctcatacgg ctcgacgttt ttacgcagca
    16861 gcgcgcgtac ggaaatcccc atatcggcaa tgagtttgac cagttcatca cgcgtcggtg
    16921 gggtttccag ataatagacg atggtcggtt ctgtaccgct gttgcggatc atctccagcg
    16981 tattacgcga cgtaccgcag gccgggttgt gataaatggt aatgttgctc atatcaatat
    17041 ctcattacaa agtgaaagag agacgtagcg ccagcgcagc cagcgtcaca aaaagcaccg
    17101 gcagggtcat gataatccct gtacggaagt aatatcccca gctgatagtc atattcttct
    17161 gcgaaagaac gtgcagccag agtagcgtag ccaggctacc aattggggta attttcggcc
    17221 ccaaatcgca gccaatcacg ttggcataaa ccatcgcttc tttgataacg ccagatgccg
    17281 tgctgccatc aatggacaac gcgccaacca gtaccgtcgg catattgttc ataatagaag
    17341 agaggaaggc ggtgaggaat ccggtgccga gcgtcgcggc ccacaggccg ttttccgcca
    17401 gcacgttgag tacgccagaa agatattccg ttagtccggc attgcgcagg ccataaacca
    17461 ccagatacat gccgagcgag aagatgacaa tctgccaggg cgcaccgcgt agcaccttac
    17521 ccgtattaat tgcatgaccg cgtttagcga cggcaaataa tatcagcgcg cccactgccg
    17581 caatggcgct gaccggaatg ccgagcggtt ccagaacgaa aaatcccacc agcagaagca
    17641 gtaaaacaac ccagccagtt ttgaacgtag caggatcttt aatcgcttct gcgggagatt
    17701 tcagcagcgc catgtcgtag ttctgcggaa tatctttgcg gaaatagaga tgcaacatca
    17761 ccagcgtggc aacaatcgcg gcgatatcca ccggcaccat caccgaggca tattcgcgaa
    17821 agccgaggcc aaagaaatct gcggaaacga tattcaccag gttggagaca ataagcggca
    17881 ggctggcggt atcggcaatg aacccggccg ccatcacgaa cgcaagcgtc gtgcctttac
    17941 tgaaccctaa agccagcagc atggcgatga caatcggtgt caaaataagc gccgcgccat
    18001 cattggcaaa cagagctgca acggcagcac cgagcaggac aatccaggta aacagcaagc
    18061 gaccgcgtcc attgccccag cgtgagacgt gcagcgctgc ccattcaaaa aagccggact
    18121 catccagcag caggctgatg ataataacgg caataaacgc agccgtcgcg ttccagacga
    18181 tattccacac caccggaata tcacccggat gtaccacgcc cgtaactaac gccagtactg
    18241 cgccgagtgt tgcactccag ccgatgccta aacctttcgg ctgccagata accaatacga
    18301 tggtcaggac aaagatagcg cctgccagta acataatgcc tcccggaaaa aacacatgtg
    18361 caaatccata tgtgtttaga taaatttttt aactacaaat gttcttactg tcttcggaac
    18421 aattttgtcg agccaggttg cggacaatca cctgaacctt ttcctgttcg catcgccagg
    18481 cctgctcaat aattttcgcg gcccatgatg gaatatgcgg tgataagcgg taatgaaccc
    18541 acttaccctg cttgcgatcc agcaatagcc cactttcacg cagcaatgcc aggtggcggg
    18601 atatcttggg ctgcgactga tcgagggcag tacagagatc gcagacgcat aattctccca
    18661 gctcgctgag cagtaaaacg atgcccagac gggtttcatc agcaagaatt ttgaacagtt
    18721 ggatgggtaa cagaaatgac atattgcgct cctgattgtt gcaggtagtg tctctcttcg
    18781 aagcggataa gtcaaaaaca tatatggttt aacgaatgtg tatgtgcaga ggaaggtaat
    18841 aggtgtgaat tttgagttgg ctattcattt gaaaggaggt ctgaatcagg acgtaaaaaa
    18901 accgactttg cgtcggtttt ttactttcca gccctgagtt ggtggctctg gctggagtga
    18961 gagagctctt atctaacagt tcaatactat tagccagtgg ctaacattgc aagaattagc
    19021 gatttcttca gctggagaag ggacagtttg ctatacatgt tggtgaaaga ggatttatat
    19081 actttcatcg ggtcgctgcc gtaccaagcc ttgtccgaat ccggacgtaa aaaaaccgac
    19141 ttcgcgtcgg ttttttactt tccagccctg agttggtggc tctggttgga gtgagaatgc
    19201 catcatctaa cgcatcaatg ctaataccat actgaaacta ttgcaaggac gtgctggttt
    19261 tataacctgc atgtactgta tgattatcca gttagctctg aggcattttc actctggtaa
    19321 tgcgcataaa cgctttcaaa gtcctggtca gaagtacggg tggtgccgtt aactgatgct
    19381 ctggccggag tgagagagtt cttatctaac aatgagacat gcgccgtgac aggcagtgga
    19441 tgagtaagcg gatgcattct cactccatag catggagaaa acgggtgatt gataaagcaa
    19501 tcatcgttct tggggcgtta attgcgctgc tggaactgat ccgcttcctg cttcagcttc
    19561 tgaactgata gcggaaacgt agctaagggc aaagagcaca ccactctttg ccctttaaca
    19621 tttaacgcat ggtcacgaac tcttctgccg ccgtcgggtg aatggcgacg gtattgtcga
    19681 agtctttttt ggtcgccccc atcttcagcg ccaccgcgaa gccctgcaac atttcatcca
    19741 taccaaaacc aatgccgtga attccgacaa tcttctcttc cggtccaacg cataccagtt
    19801 tcatgcggca cggctggcgg tgagtggtga cggcggtata catcgcggtg aaagaggatt
    19861 tatacacttt cacctgatcg tcgccgtact gctcgcgcgc ctgcggttcc gttaaaccaa
    19921 cagtcccaat cggcggatgg ctgaagacca cggtcggaat gttgctgtaa tcgagatgct
    19981 catccggctt gttattaaac aggcgttcag agagacggcg acccgctgca actgctaccg
    20041 gtgtcagttc cactgcaccc gtgttatcgc ccaccgcgta aataccttca acgttggtat
    20101 tttgatattt atcgacgacg atatagcctt tttcgttagt tttaacgcca gcggcttcca
    20161 ggttgatgtt gtcattggca ggctcgcgac caatcgccca aatcaggcaa tccaccgttt
    20221 cactgcgccc atcttccagc tccagcgtca ggctaccatc agcatttttc actactgctt
    20281 tcgggatggc gttggtgtgc aactgcgggc cttcggcgtt catcacttcg accagcgttt
    20341 cagagatcat cgggtcgaag ctgcgcagcg gcgcatgttt acgcacaaac agatgcgttt
    20401 tcgcgccgag gccgttaatc acgcccgcca gctcaacggc gatgtaaccc gcgccaacaa
    20461 ccgccacgcg ctctggcaaa gcaggaaggg caaagaagcc atcagaatca ataccgtatt
    20521 ccacgcctgg aatatccggg tggctcggac gaccgccggt ggcgatcaga atatgatcgg
    20581 ccgtgatggt ttcgccgttt acctccagcg ttttggcatc aacgaagcgg gcaaagcctt
    20641 tgattacatc aacgttattt ttaccgagca cgttttcata ggaagtatga atacggtcga
    20701 tataggcggt acggctggcg atcaacgttt cccagttgaa tttatttata gtggtatcga
    20761 aaccataatc cggaccgtac atgtggatcg cttcacggat ttgcgccgcg tgccacatca
    20821 ctttcttcgg tacgcagccg acgttcacgc aggtgccgcc cagttctttg gcttcaatca
    20881 gcgcgcattt ctgaccgtac atagccgcgc ggttgatgga ggcgataccg ccgctgccgc
    20941 cgccgatggc gatgtaatca tagtgtttag tcatgacaga gtgtccttat cgttgattac
    21001 cgcgattgta gcgcgagacg taataggtgc cagcaatggc tgcaattact ccggcacgat
    21061 ccagcttacg gtggcgtgcc cggtgcctgc cggaaccagt ttgctgtgca gccacggcag
    21121 cacgttattc atctgctgtt ccagcttcca cggcgggtta atcacaatca tgccggaagc
    21181 ggtcatgcca cggcgatcgc tgtctggcag taccgccagt tcaatttgca gaattttgcg
    21241 aatgccggtc gcttccaggt cgtggatcat gcgcttaatt tgctggcgca acaccactgg
    21301 ataccacagt gcgtaagtgc cagtggcgaa acgtttatag ccttctgcta tcccgctgac
    21361 caccgcctga tagtcggttt tcatttcata cggcgggtcg ataaggatta aaccacggcg
    21421 ggaaaccggc ggcagtttgg ccttaagctg ctggaaacca tcggcttttt cgacgcgcgc
    21481 acgattgtct ttctgaaatt cagaacgtag caacgggtaa tcgctcgggt gcagttcggt
    21541 cagttgcagg ctgtcctgtt cacgcagtaa ctgacgagca atcaacggcg aaccggggta
    21601 gtaacgcaac tgaccgctac ggttgaagtg ttttaccaca ttgatgtacg cctccagctc
    21661 tgcaggcaaa tcgtcctgct gccagatacg ggcgatacct tcgagatatt cgccggtacg
    21721 ctcggcatgt tcgctgccta actgataacg cccggcccct gcgtgggtat cgagatagag
    21781 aaacggttta tctttctctt tcagcgactc gatgatcagg ctctgaacgg tatgtttaag
    21841 gacgtcggcg tggttgccag cgtgaaagct gtggcgataa ctgagcatgg gtaaaggtgt
    21901 tccggtaagt aaaaatcggc ccgtattcgg ggcgcacaaa agcctatcag gacagtatac
    21961 cgaaaagagg ccgccgccgc gaaagcgtaa cgtttctcat tgaaattcac tacacttaac
    22021 cccatgctac aaacattatg taaagcgcct gttgagcgct tccttaacct ctttaaccag
    22081 gactgcgcta atgacgaatc cgttactgac tccctttgaa ttgcctccgt tttctaaaat
    22141 tctcccggaa catgtcgttc cagccgtgac taaggcgctg aacgactgcc gcgaaaatgt
    22201 ggagcgcgta gtagcgcaag gggcaccgta cacctgggaa aatctctgcc agccgttggc
    22261 ggaagtggac gatgtgctgg ggcgtatctt ctccccggtc agccacctga actcggtgaa
    22321 aaatagcccg gaactgcgtg aagcgtacga acaaaccctg ccgctgctgt cagaatacag
    22381 cacctgggta ggacaacatg aagggctgta taaagcatat cgcgacctgc gcgatggcga
    22441 tcattacgcc acgctgaaca cggcgcagaa gaaagcggtt gataacgcac tgcgcgactt
    22501 tgaactctct ggcatcggtc tgccgaaaga gaaacagcag cgttatggtg agattgccac
    22561 tcgtctttcc gagctgggca accagtacag caacaacgtc ctcgatgcga caatgggctg
    22621 gaccaaactc gttaccgacg aagcggagct ggcggggatg ccagaaagcg cgctggctgc
    22681 ggcaaaagcc caggctgaag cgaaagaact tgaaggctac ctgctgacgc tggatatccc
    22741 aagctacctg ccggtaatga cctactgcga caaccaggct ctgcgcgaag agatgtatcg
    22801 cgcttacagc acccgcgcct ccgatcaagg tccgaacgcc ggtaagtggg acaacagcaa
    22861 ggtgatggaa gagatcctcg cgctgcgtca cgaactggcg caactgctgg gctttgaaaa
    22921 ctacgccttt aaatcccttg ctactaaaat ggcagaaaac ccgcagcagg tgctggattt
    22981 cttaaccgat ctggcaaaac gcgcgcgtcc acaaggcgaa aaagagctgg cgcaactgcg
    23041 tgccttcgct aaagccgaat ttggcgtgga tgagttgcag ccgtgggata tcgcgtacta
    23101 cagcgaaaaa caaaaacagc acctctacag catcagtgac gaacagctgc gtccgtactt
    23161 cccggaaaac aaagcggtta acggcttgtt tgaagtggtg aaacgtattt acggcatcac
    23221 cgctaaagag cgtaaagatg ttgatgtctg gcatccggat gtacgtttct tcgaactgta
    23281 tgacgagaac aacgaactgc gcggcagctt ctacctcgac ctgtatgccc gtgaaaacaa
    23341 gcgcggcggg gcgtggatgg atgactgtgt aggccagatg cgtaaagccg acggttcgct
    23401 gcaaaaaccg gtcgcgtatc tgacctgtaa cttcaaccgc ccggtaaatg gtaaaccggc
    23461 gctgtttacc catgacgaag tgatcaccct gttccacgag ttcggtcacg gcctgcacca
    23521 tatgctgacc cgcattgaaa ctgctggtgt atctggtatc agcggggtgc cgtgggatgc
    23581 ggtcgaactg ccgagccagt ttatggaaaa ctggtgctgg gagcctgagg cgctggcgtt
    23641 tatctccggt cactatgaaa ccggcgaacc gctgccgaag gagctgctgg ataaaatgct
    23701 ggcggctaag aactaccagg cggcgctgtt tattctgcgc cagctggagt tcggcctgtt
    23761 tgatttccgc ctccatgccg agttccgccc ggatcaggga gcgaaaatcc tcgaaactct
    23821 ggcagaaatc aagaaactgg ttgccgtagt accgtctcca tcctggggcc gtttcccgca
    23881 cgctttcagc catattttcg ccggtggtta tgccgcaggt tactacagct acctgtgggc
    23941 cgacgtgctg gcggcagatg ccttctcgcg ctttgaggaa gagggcattt tcaaccgtga
    24001 aaccggacag tcgttcctcg acaacattct gagccgtggc ggttcagaag agccgatgga
    24061 tctgttcaaa cgcttccgtg gtcgtgaacc gcagctggat gcgatgctgg agcattacgg
    24121 cattaagggc tgatcattca gtgaaaatct gcttaattga tgaaacaggc accggagacg
    24181 gtgccttatc tgttctggcg gcccgctggg ggctggagca cgatgaagac aacctgatgg
    24241 cgctggtatt aacgccggag catctggaac tgcgcaagcg tgatgagcca aaacttggcg
    24301 gcatcttcgt tgattttgtt ggcggagcga tggcgcaccg acgcaaattc ggtggtggtc
    24361 gcggtgaggc ggtggcgaaa gcggtgggca ttaaaggcga ttatttgccg gatgtcgtcg
    24421 atgctaccgc agggctgggg cgcgatgcct ttgtgctggc ttcagtcggc tgccgcgtgc
    24481 ggatgctgga gcgtaatccg gtggttgctg cattgctcga cgacggcctg gcgcgtggtt
    24541 atgcggatgc ggaaatcggc ggctggttgc aggagcggtt gctgttaatt cacgcctcca
    24601 gcctgacggc gctgaccgat attaccccac gcccgcaggt ggtctatctc gatccgatgt
    24661 tcccgcataa gcagaaaagc gcgctggtga aaaaagagat gcgtgtcttt cagtcgctgg
    24721 tggggccgga tcttgatgcc gatggattac tagagcctgc acgcttgctg gcgaccaaac
    24781 gcgtggtggt aaagcgcccg gactacgcgc cgccgctggc gaatgtcgcc acgccaaacg
    24841 cggtagtgac caaagggcat cgctttgata tttatgccgg aacgccggtg taaaagtaaa
    24901 gcagggtagc gttatcgcta ccctgctttc tgcaagaata attaatggct ttcgggcgtc
    24961 gcaatcattc gtttcagcca cggcaccatc agcagcatca ccaccgcaac gcccagcgta
    25021 accaggccaa tcttaccaaa cacgttggta tagacgggca acgtctcaag cggatcggta
    25081 atgttgtctg gtactgcggt aaatgttgcc acatagccgc ccagcaagaa cgcggcagcc
    25141 tgcgtcagga accacatccc gagaataaag cccatcaaat gctgcggcac cagggcagca
    25201 atcatcgcca gacccagggc gctaataaac aattcaccca ggctctggaa taagtaaacc
    25261 agcacaataa accacggcga tgtcagtcct tgggcatccg caaaccacat tcccgcagct
    25321 gcagccgtca aaaagcccag tgagcacata aacatgccga gagtaaattt catcggcata
    25381 gagaggtctt tgcctttgtt acccagatgc gtgtaaatgc ctgccagtat tgggctggcg
    25441 agtaccaccc agaacgggtt aagtgcctgg aagctgaccg ggttgatgga aaagccgaga
    25501 atttcatgat gcacgttgtt gatggcaaag aagttcagcg acgttggcat ctgggcgtag
    25561 agaatgtaaa acaccaccgc ttcgagcatc aggacaaagg cgacaaacat tttattgcgc
    25621 ccggttttat ccagcttgaa tgcctgacga aagaaaatga tggtgacgac gatagagaga
    25681 acaatcagca ccagattggc gacttctacg ttgtgcatca gccatgcaca gacgaagatc
    25741 atcaccacgc tgccaagtaa cacgtacaac agtttgctga agctcatcgg ccggaagtcg
    25801 ggttcagaac caatgtcttt caccattccg cgacaggcga tgtaaaccaa tagcgcgata
    25861 attaaccccg caccgcacag gttgtaggtg actgaataac cgaatctatc agcgatcacg
    25921 ggggccagcg ataacgctat caacgagccg atgttgatcg acatatagaa cagggtaaat
    25981 gcgccatcaa gccgcggatc tttcggcgga tagcacttcg aaagcaagct ggctgggtta
    26041 gctttaaaca ggccgttacc gacagcgata gtccccaggg cgatgaaaat caggtcaggc
    26101 ttaagtagcg acaggccggt catgaagtag ccaatcgcca gcacaagtgc tccgagaaca
    26161 atggtgcgtt tggtccccag caggtggtcg ccgacatagc cgccaatgga aatgaggcca
    26221 tagaccagcg cagcaaaagc accaaaagtg acaaaagcct gttcttgcga gaatccaagc
    26281 tgcttaacga agaaaaccgc cagtacgccc tgcacgccgt agtagccgaa tcgctcccat
    26341 aactcgacaa aaaagatcat gaaaaatggg cgaggttgct gcagcatccc catgggtgtt
    26401 gttgtattca tatttattaa ccttccaata ccatcctgaa aagtatgaac gcgtaaggga
    26461 taacgcgcga aaaagttttc atctgtcaga gattcttaat attgatgcgg tgaatagatt
    26521 aatacactat tacaacagaa aataaccggc tgatcacact aatgatggaa aatatgatgg
    26581 tgttaaatgt tgtattggca ggggattttg attgcgtacg cagaccgtag gccagataag
    26641 gtgtttacgc tgcaaggtaa caaggcgcgc caaaaagcaa aacgcccgca tatgcgggcg
    26701 ttcaggaaca cgtcgtagag ggaagattat tcttcttcgt cgcgcagcgg aacaatcagc
    26761 atatcaacgt gaacggtgtt gatcagctga cgtgcggaag acatcagttt gctccagaag
    26821 tcctggtggt gaccacaaac caccagatcc atatcgtatt tcttgattgc atcgaccaga
    26881 acctggccca gatcgccgct gccgctcagg gtttcagtga ttgggtagcc tgcattagtg
    26941 gaaagctcgg tcagcgcatg atgtgtctct tcagagatgc gtttctgcat atcacccaga
    27001 ttcacatcaa taagcccggt gtataggtca gagtagttta catctacgtg gatcagagaa
    27061 actttcgcat tgtaggggcg agccatagag actgctttct ctaccagaac tttgctttcc
    27121 ggggagagat cgaccgcgat gagaatgtgt ttataagcca tagtgttact ccttccataa
    27181 agttgtcgat gactggccag ctagcgtttc ttgtgccgtt acgtcacccg cgtcctgcga
    27241 actacgtgcg cggggctaac ccagccgtaa agattcagtg ttaagaccat ccttacctta
    27301 tagcgacccg gatgatccgt caatccgcct tgcttacccg ttagataaac aaattttcga
    27361 catgaatata ttgatagtgg ttaaccttct ggaaaaaaaa caacctgatc tcctacacta
    27421 tctatagagc cgctcggatg ttggtcacca caaaagcggt ttctccagga cctggtgtta
    27481 actgattggc agcgtatcgg gagcggtagc cccggggtgg aactccgcgg gcaggtcgcc
    27541 ggggaggagg tatgataagc accgtcgcat tattttgggc tttatgtgtc gtttgcattg
    27601 ttaacatggc gcgctatttc tcatcactac gcgcgttgtt agtggtactg cgtaactgcg
    27661 atccattgct ctatcaatat gttgatggag ggggcttttt tacctcacat ggccaaccca
    27721 acaaacaggt gcgtctcgtt tggtatatct atgcccaacg ttatcgcgat catcacgatg
    27781 atgagtttat tcgccgctgt gagcgggtgc gtcggcagtt tattctgacc agcgcattgt
    27841 gtggtctggt ggtggtcagt ctgattgcat tgatgatttg gcattaagca cttcagcaaa
    27901 aataacgcat aaaaaaagcg ggccagtaaa ctgacccgct cgttttggtg cgtgcgatta
    27961 caggaactgc aaggagagcc agtaaagccc gccggaaagc agtaccgcag ccggaagggt
    28021 aaacacccag gccatcagaa tactggtcac ggttttacgc tgtaagccgc cgccatctac
    28081 caccatcgtc cccgcgacag aagaggagag tacgtgggta gtggaaaccg gcatcccggt
    28141 ataactcgcc aggccgatag acactgccgc agtcatctgg gcagacatcc cctgagcgta
    28201 ggtcatgcct ttcttaccga ttttctcacc gatagtcgtt gccacacgac gccagccaat
    28261 catcgtaccg atacctaacg ccagcgcgac cgccatgatg atccacaccg gcgcatactc
    28321 gatggtgcta agcatatcgg atttcagttt cttcaacagg cgctgatcgt cagcactcac
    28381 gccaggcatc ttcaccactt tgtcgatagt gtcagaaacg cacagcataa tgcggcgcat
    28441 ctggctacgt tgatcaagcg acagcttgtc gtagctttcc acatcggtag tcaacatgcc
    28501 tttcaggcgg ttgagcgcgt taatggtatt cgacggatgg caatggaact ccgcaggttg
    28561 cgttgcgcca gcttccggag ccggtactaa ctgatcagca ccggtagcct gtttcagcag
    28621 cgcagggtgc tgctcaaagt aagcttccac gttgttgatg gcatcacggg tacgggtgat
    28681 ttcgtagcca gtggcattca tgttcaccac gaagcctgct ggtgcgacgc caatcaatac
    28741 caacataacc agaccaatgc ctttctgacc atcgttcgcg ccgtgcgaaa acgccacgcc
    28801 gatagcggaa aggatcagcg caatacgcgt ccagaacggc ggctttttct tgccgtcttt
    28861 cttttcacgc tccgctgggg tcaggtggat acgggcgcgt ttcttggtgc cgctccagta
    28921 gcgacgcagc aagaaaatca gaccgccagc aaacaccagg ccgacaatag gggaaacgat
    28981 cagagaaccg aaaatactta atactttcgg gatattgagt gcatccacca ctgacgtccc
    29041 ggtcatcaac gcattggtta aaccaatccc gatgatcgcg ccaatcagcg tatgagagct
    29101 ggatgcaggt aaaccaaagt accaggtacc caggttccag ataatcgccg ccagcaacat
    29161 agagaacacc atggcaaggc catgagacga tcccatatta agcagcagat ccgtcggcag
    29221 catatgcaca atggcatagg caacactcag accacccagc aaaacaccca aaaagttgaa
    29281 caccgccgcc ataaccacgg cgagctgaga acgcatcgcg cgggtataga taacggttgc
    29341 cacggcgttg gctgtgtcat ggaaaccatt gatggcttcg tagaacagca caaaagccag
    29401 tgcaagcaat aataacagcc cggtatgcaa atccaggcca gcaaacaaat gtagcatagg
    29461 acgttacgcc attttgagga catgaacgcg gcgcattatc agtgactttc gcggcgcggg
    29521 caaagtgaaa tatagacttt ttttgatttg cctcctgtat ggatttcact caaaaaataa
    29581 ttatcttata taattcaggc aaatacttcc ttttagtaat attgatgctg gtgcgaccac
    29641 tgaggaatct ttacaattca cgcccgtttt ttcttagagg agcgcaacgt ggaaaggttt
    29701 gatgccatta ttataggcgc tggtgcggcg ggtatgttct gttctgcgct ggcaggtcag
    29761 gcaggacgcc gggttctgct gatcgataat ggtaaaaaac cagggcgcaa aatcctcatg
    29821 tctggcggtg ggcgctgcaa ctttaccaat ctttatgtcg aaccgggcgc ttatctgagc
    29881 cagaatccgc atttttgtaa gtctgcactc gcgcgtttta cccagtggga tttcattgat
    29941 ctggtcaata aacacggcat cgcctggcac gagaaaacgt taggacaact cttctgcgat
    30001 gactccgcgc agcagattgt cgacatgctg gtggatgagt gcgagaaggg caatgtgacc
    30061 gtcagattgc gtagcgaagt gctgagcgtg gcgaaggatg aaacaggctt cacgcttgaa
    30121 ctgaacggca tgactgtcgg ttgcgaaaag ctggtcatcg cgaccggtgg actgtcaatg
    30181 ccggggctgg gcgcgtcgcc gtttggttat aagattgccg aacaatttgg cctcaacgtg
    30241 ctgccgaccc gcgcgggcct ggtgccattc actctgcata aaccgttgct cgaagagtta
    30301 caggtgctgg cgggcgtggc ggtgccttcc gtgattaccg ctgaaaacgg caccgttttc
    30361 cgtgagaact tactcttcac ccaccgcggc ttatctggac cggcggtgtt gcaaatttcc
    30421 agctactggc aaccgggtga atttgtcagt atcaatctgc taccggatgt ggacctcgaa
    30481 accttcctga atgagcagcg taatgcacat ccgaatcaga gcctgaaaaa cacactggcg
    30541 gttcatctac cgaagcggtt ggttgaacgt ttacaacaac tcgggcaaat cccggatgtt
    30601 tcgctaaaac agctcaacgt gcgtgaccaa caggcactga ttagcacatt gaccgactgg
    30661 cgcgtacaac ccaacggcac tgaaggctat cgcactgccg aagtgacgct cggcggcgtg
    30721 gacaccaacg aactctcttc acggacgatg gaagcgcgca aagtgcctgg gctgtacttc
    30781 atcggcgaag tgatggacgt caccggttgg ctggggggct ataacttcca gtgggcgtgg
    30841 tcgagtgcgt gggcttgtgc gcaggatttg attgcagcga agtcgtcctg agctatttcc
    30901 taaagatgaa aattattaga gtcgcattaa aaatgagcta attttgatag tggttatctt
    30961 gtgattattt tctaatgagc ctgtgaactg agaccctcca tgcttaatat aaggtggagg
    31021 gaaaggtgat taaaactccc ccagtggcca gatctttata aaatatgaac aggttgaaaa
    31081 aatcagcaac ttagctgttt atttatactt aaccactatg cattaatgct gcgttatttc
    31141 atgataccta agaaaaacca gcgttacgca aataatcaac gctggtttta tccggtacgt
    31201 tgcaattatt ttttagcaga acccgcttct aatattgaaa cgattgagaa caacgtaaag
    31261 cataccgctc ccaaaccgac cagaacccgc gaaggaataa aatatcccat atcggtctgc
    31321 gccatttccg caagaaacga tgccaggaaa aggcaaaaca ggcaggtgaa gacgggaatc
    31381 atcggtattc tgttggctaa cgaacaggta cgtctccaga ccagtgccag tagccagact
    31441 tttgagaata tgctgtaaca gatcattccg aggcaaatga ggataatgcc gggagccagt
    31501 cgggcgcttg catcggagct gactaacacg tatatgccct gcagcaccgt gattgagccg
    31561 agaataataa cccaataaca ccacagccag tgttctttgg atgagaacgt attacgtgtt
    31621 tgatggacga ttgtggcaac aaggccaatt agacaggcac agattgcggt taacccaagc
    31681 aatacgtgac cggcgacata atgcggagtt atgtcggcac tacgtaacag cgtaatagac
    31741 caaatgaaac cgagcagggt gagtaacacg ggaacggcaa tgaggcaatt accgattaat
    31801 gacgaataag cctgtaccgg tattccatcg ctcttgctcc ctgctgcgtt tttgggaatg
    31861 agcagaaagt gaccggatga cgctgccact gtcgatacac aggcggcaat cataccaacg
    31921 ccaaaaataa catggccggc aacaaactcg tctgccatca catcattgcc tgctaacagt
    31981 gcccaacccc atatcatggt gataattgac cccgcatagc caataatggg gaacaatgta
    32041 ttgtaaaacg tattaacgcc gcgcgtgagc tgcgaaatga taataaatgc ggtagtgaat
    32101 aatgccaggc atatggctgc cagagaaatc agcacatgac ccgcgacgaa atactcactt
    32161 tgcccagaac tttccagcac aaagccacct aacgcgatac aaattaagcc cataattaaa
    32221 gggattaatt tgaaaagcca cccgatatat atgttcactt taaactcctc aggtatccct
    32281 gctaatattg aaatgtgcat taatgataat ttcagcctgt taatataatg acgagatatc
    32341 attaaagaca aatgcatagc ataactatag cgggatttac tttacagagc gctacttttc
    32401 agcgttcaaa aggccatggt aaataaatat ttatcctaag attaattctt attctgttgt
    32461 tttttacaat tatcttccag ggagggattc tttttcatta tttggtaaaa gaccggatgg
    32521 tatcggtgct taaaatcatc gaacatataa gtattattgc tttgtatcat cgtaaatatg
    32581 tctttcagac atgtgtcatt tattttgtta ctaacaagtc aaaaatatca cgggttattt
    32641 atgaaagtat ttcacaatat atttaagcac ttctcttcaa accatcaaga taagcattca
    32701 gataaagtta acagtcatca acaccatggc aaggttgata aaaaacatcg ttcaaagata
    32761 gttgaaattg ataagctgga taaacattcg caaatagata acgattttgg cctgcatatc
    32821 atctatttcc tgcaacatgg ctgctggaac gtaactgaac atagtcctca gatagaaaaa
    32881 ctatggtttt acaatagtga gccatctata gatattaaag aatataatag tttcgccgat
    32941 aatactactg acacgtttat ctttacaatt ataccagaca ataaccatgt gataaaatta
    33001 tcgtctccca ttaccgttac gattgagcgt aaggacggtt attattttat taatttctcc
    33061 ggagataaat cagacataat ttataaagtt gatgggtttt ctatcataga gagaaatttc
    33121 tttactcttc ttagtggtaa tttcaaacct gactggcgtt gggctgtctc taaagagacg
    33181 tttactaagg aaaagtttga tagctatgtt gaatctgttt tttcaaaaat agacttttat
    33241 aaacagtgtg gtgtgattaa tccacaaaat gccaacacgg cttattttgg cgatacagat
    33301 ggaagagtag gcgctgtact ttatgcattg ctcgtttcag ggcatatagg catcagggga
    33361 gaggggtgga gtttattatg tgatttatta aaacacgaag acatggcttc attcgcatat
    33421 gaaaataaaa aattgaaaaa actcttcact ttattagata cgagagacat gatcttgaat
    33481 gagttgcatc aacatgtttt tttaaaaggc gatgcgatta ccccttgtat cttccttggg
    33541 gatcatacag gcgatagatt tagtactatt tttggtgata aatatatcct tactttgcta
    33601 aattctatgc gaaatatgga gggtaataaa gatagtcgta taaataaaaa tgtcgtcgtt
    33661 ctggctggaa atcatgaaat caattttaat ggtaactata gtgcacgact agcaaatcat
    33721 aaactctctt ccggtgatac atatgactta attaaaacgc tcgatgtttg taattatgac
    33781 tcagaaacga aagttttaac cagtcatcac ggaataataa gaaatgaaga aaatgaaagt
    33841 tattgtctag gtgctttgca gattccattt aatcagatga aagacccaat tgatcctgag
    33901 gaattagcaa atatatttaa taaaaaacat aaacaacaca tggatgatcg tcttatccat
    33961 ctaattagaa gcaatgctat agcatccaca cctgtatatg acaattattt taataacact
    34021 actgctttca gaccaaaacc tgaagatatc ttcaaatgtg gacaaacgct caaaaaaagt
    34081 aaacagaaat atggccatta tggattaggg gtagatcagc atcaaaaaat tgataattat
    34141 accatggggc taaattcctg gaagatagca cctaacgaac gtggtgataa aaaaggtgtc
    34201 ccagggctta gttgttttca atctcattaa aactccatga atacggtggt atattttttt
    34261 aatcctgcca gtatttcata gtttttctta atcgattgta gaggcatgta ctgatgacgc
    34321 ctctacaatc tttagggact ttctctacta tgagataaga aatttcatat tatgctttaa
    34381 ggctgaatga tttgtgcaaa atataattat tttcccgtcg aacatgaggt gtatgtcata
    34441 agattaaatg ataactatgt attccttgcg gtgaattata tgaggtaaaa gatgaaactt
    34501 ggaactgtgg caggcactaa cgacagtaca acgacaatcg caacaaatga tatggtgcaa
    34561 gagcatgtta ctaacttcac taaagaactg tttgggtata ttgcaaatgg tataggtgat
    34621 gatatatcaa gtattgccag gacaatgctc ggtgaagttg tggaaaaaat tgatgattgg
    34681 caaatagaac gtttccaaca atcttttcaa gatgacaaaa tttcatttac catagaaacg
    34741 aatcattcag aaaaatatag tatgctatcg ggcatgcgtg cccatattct acgtaggaat
    34801 aatagttatc agtttattgt gacaattaat agtaaaaatt acggttgccc tcttgatgat
    34861 accgatatta attggtgcag cattgtttat ttacttaata atatgacagt aaatgataat
    34921 gctaatgatg ttgcagttac tgagtcgtac aaacccatct ggaactggaa aatatcgcaa
    34981 tacaatgtat ctgatattaa attcgaaaca atgataaaac cccagttcgc tgacagaacc
    35041 tatttctcca attgttcacc tgttgatccc acaagtacca gaccaaccta ttttggcgat
    35101 accgatggct cagttggtgc agttttgtac gcgcttttcg caacgggaca tttgggaatc
    35161 atggctgaag gagaaaattt ccttggccag ttgcttaata tagaagatga agttctcaac
    35221 gtattgctaa gggagaactt taatgagcaa cttgacacta atgttaatac aataatcagt
    35281 attctgaaca gaagagatat agttttagaa agtcttcagc cttatttagt tattaataaa
    35341 gacgctgtga cgccatgtac gtttcttggc gatcaaaccg gcgatcgatt tagtaatatc
    35401 tgtggcgatc aatttatcat cgatttgtta aaacgcataa tgagtattaa taaaaatgtt
    35461 catgtattag ctggtaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg
    35521 cgtatgaaac cacttggtga ggatacctac gctgggataa aagattatcc tgtatgtttc
    35581 tatgacccca aatacaaaat aatggcaaat catcatggga taacttttga tgatcagcga
    35641 aaacgttaca ttataggacc gataacagtc tctattgatg aaatgaccaa tgctctcgat
    35701 ccggtcgaat tagcggcaat tattaataaa aagcatcata ctataattaa tggtaaaaag
    35761 tttaaaacta gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcgaca
    35821 gattacagac ctaaacttga agcactatta gcatgctcac aaatgttagg tataaatcaa
    35881 gttgttgcac ataacggaaa tggtgggaga gaacgcatag gtgaaacggg gacagtttta
    35941 ggtcttaacg cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa
    36001 attaaccctg gtgcggggcc tgagataacc actccctgga agtcttacca gcatgaaaaa
    36061 aacaaaaatg gacttatgcc tttgattaga aggcgcacaa tgttacagct ctgaaccata
    36121 gacaacatga atattaatgt tactatctgg ttgagactgt tgataaaacg taataaggaa
    36181 tgctctgttc ctgcaactga acaggcgtga aacgctatcc aacaggatgg ataccgtttt
    36241 gtcagccagt tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg
    36301 ctgatgatga caacattact acccgttttc actaagcctt ccacgttggc gctcaatgct
    36361 ctgcgcgctg gtcgtatttg ccgtttcctt cttatcccgg atggaagaat ccgctgaacg
    36421 tcgttcggcg gcagttgtaa cacgcgtcgt ttgcttacag ggattatggc gggtcgatta
    36481 tggataagag taagcgccat ctggcgtggt gggttgtcgg gttactggcg gtggccgcta
    36541 tcgtggcgtg gtggctgttg cgcccagcgg gtgtgccgga aggctttgcc gtcagcaatg
    36601 ggcgcattga agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt atcgacacca
    36661 ttctggtgaa agaaggccag tttgttcgcg aaggtgaagt gctggcgaag atggatactc
    36721 gcgtgttgca ggaacagcga ctggaagcca tcgcgcaaat caaagaagca caaagcgccg
    36781 ttgccgccgc gcaggctttg ctggagcaac gccaaagcga aactcgtgcc gcacagtcgc
    36841 tggtcaatca acgccaggcc gaactggact ccgttgccaa acgtcatacg cgttcccgtt
    36901 cgctcgccca acgaggggct atttctgcgc aacagctgga tgacgatcgc gccgccgctg
    36961 agagcgcccg agctgcgctg gaatcagcaa aagcccaggt ttcggcttct aaagccgcta
    37021 tagaagcggc acgcaccaat atcattcagg cgcaaacacg cgtcgaagcc gcacaagcca
    37081 ctgaacggcg cattgccgca gatatcgatg acagcgaact gaaagccccg cgtgacggac
    37141 gcgtgcagta tcgggttgcc gagccagggg aagtgctggc ggcaggcggt cgggtgctga
    37201 atatggtcga tctgagcgac gtctatatga ccttcttcct gccaaccgaa caggcgggca
    37261 cgctgaaact gggcggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc
    37321 ctgcaaccat cagttttgtc gccagcgtcg cccagttcac gccaaaaacc gtcgaaacca
    37381 gcgatgagcg gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc
    37441 agcagcatct ggaatatgtc aaaaccggtt tgccgggcgt agcgtgggtg cgggtgaatg
    37501 aagaacttcc gtggcctgac gacctggtgg tgaggttgcc gcaatgacgc atctggaact
    37561 ggttcccgtc ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaccgt
    37621 tgcgctgaac aatatcactc tcgatattcc gacccgctgc atggttgggc tgattggtcc
    37681 ggatggcgtc gggaagtcga gtttattgtc attgatttcc ggtgctcgtg tcattgagca
    37741 gggcaatgtg atcgtgctgg gcggcgatat gcgcgacccg aagcatcgac gcgacgtttg
    37801 cccgcgcatc gcttggatgc cgcaggggct gggtaaaaac ctctaccaca ccttgtcggt
    37861 gtatgaaaac gtcgattttt tcgcccgcct gttcggtcac gacaaagcgg agcgagaagt
    37921 gcgaattaat gagctgctga ccagcaccgg gttagcaccg tttcgtgatc gtccggcagg
    37981 gaaactctcc ggcgggatga agcaaaaact ggggctgtgc tgtgcgttaa tccacgaccc
    38041 ggaactgttg attcttgatg agccaacaac gggggttgac ccgctctccc gcgcccagtt
    38101 ctgggatctg atcgacagta ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac
    38161 cgcctacatg gaagaggccg aacgcttcga ctggctggta gcgatgaatg ccggagaagt
    38221 gctggcaacc ggcagcgccg aagagctgcg gcagcaaacg caaagcgcca cgctggaaga
    38281 agcatttata aatctgttac cgcaagcgca acgccaggcg catcaggcgg tagtgatccc
    38341 accatatcaa cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt
    38401 tggttccttc gttgccgttg atcacgtcaa tttccgcatt ccacgcgggg agatttttgg
    38461 ttttcttggt tcgaacggct gcggtaaatc caccaccatg aaaatgctta ccggattgct
    38521 gcccgccagc gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga
    38581 tactcgccgt cgggtaggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt
    38641 gcggcaaaac cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc
    38701 gcgagtggct gaaatgagcg agcgttttaa gctcaacgac gttgaagatg ttctgccgga
    38761 gtcattaccg ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc
    38821 ggagatgtta atcctcgatg agcctacttc tggtgtcgat ccggtggcga gggatatgtt
    38881 ctggcagttg atggtcgatc tctcgcgtca ggacaaagtg actatcttca tctccaccca
    38941 ctttatgaac gaagcggaac gttgcgaccg tatctcactg atgcacgccg gaaaagtgct
    39001 cgccagcggt acaccgcagg agttggttga gaaacgcgga gccgccagtc tggaagaggc
    39061 atttatcgcc tatttgcagg aagcggcagg gcagagcagc gaagccgaag cgccgcccgt
    39121 ggtacacgac gccacccacg cgccgcgtca gggatttagt ctgcgccgtc tgtttagcta
    39181 cagccgtcgc gaagcactgg aactgcgacg cgatccggta cgttcgacgc tggcgctgat
    39241 gggaacggtg atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct
    39301 gcgctttgcg gtgcttgacc gcgaccagac cgtcagtagc caggcgtgga cgctcaatct
    39361 ctccggttcc cgttacttta tcgaacaacc accgctcacc agttatgacg aactggatcg
    39421 ccggatgcgt gcgggcgata tcacggtggc gattgagatc ccacccaatt tcgggcgcga
    39481 tatcgcgcgt ggtacgcctg tggaactcgg tgtctggatc gacggtgcga tgcccagtcg
    39541 tgccgaaacg gtaaaaggtt acgtgcaggc tatgcaccag agctggctac aggatgtggc
    39601 gagccgacaa tccacaccct ccagccaaag cgggctgatg aacattgaga cgcgctatcg
    39661 ctataacccg gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc cgcttctgct
    39721 gatgatgatc ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agttggggtc
    39781 gattatcaac ctttacgtga ctcccaccac ccgtagcgaa tttttgcttg gcaaacagct
    39841 accgtacatc gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt
    39901 gtttggcgta ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat
    39961 catcatcgcc accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc
    40021 tattttcggt acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgattga
    40081 tccggtagct tcgctggaag gtccaggacg ctggatcggc gaggtttacc cgaccagtca
    40141 ttttctgact attgcccgcg ggacgttctc gaaggcgctg gatttgactg atttgtggca
    40201 actttttatc ccgttgctga tagccatccc gctggtgatg ggcttaagca tcctgctgct
    40261 gaaaaaacag gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag
    40321 ttgcgcagtt tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg
    40381 tcggtgtatt cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt
    40441 gccgatatgg atcaatcgca gttatcgaac aggatcgtta acagcttcta tcgcccgtgg
    40501 tttttgccac cggagatgat caccgccgat gagatggatg ccgggctgga cgccggacgc
    40561 tataccttcg cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag
    40621 ccagatattc aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg
    40681 tatatccaga atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataat
    40741 agcgaaccgt tggtatcgct ggaaacccgg atgcgcttta acccgaacct cgatcccgcg
    40801 tggtttggcg gggtgatggc gatcatcaac aacattacta tgctggcgat tgtattgacc
    40861 ggatcggcgc tgatccgcga gcgtgaacac ggcacggtgg aacacttact ggtgatgccg
    40921 ataacgccgt ttgagatcat gatggcgaag atctggtcga tggggctggt ggtgctggtg
    40981 gtatcgggat tatcgctggt gctgatggta aaaggcgtgc tgggcgtacc gattgaaggc
    41041 tcgatcccgc tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc
    41101 atttttatgg ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg
    41161 ctgctgccgc tgcaaatgct ttccggtgga tccacgccgc gcgaaagtat gccgcagatg
    41221 gtgcaggaca ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatc
    41281 ctctaccggg gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt
    41341 ggcggcgcgt ttttcaccat cgcactgctg cgatttagga agacgattgg gacaatggcg
    41401 taattacagt tgcccgatgc gtaagcttat agggtctacg cattggtgca atttattgaa
    41461 ttttagtttt ttttaggccg gataaggcgt ttatgaactg accaccaaaa cctggacagt
    41521 ttcagttaac cggctttttg atactcagtc cggtattcag ggcaagatta cgaaagcctg
    41581 ctccccgcaa ggactgacgc gagaaatgac atgaatcgct cccctgcggc gactcttgtc
    41641 ataagagtgc cggagcgttt ttccatcaat tacaatgacg tctttatcat ctgaagaatg
    41701 gcagtcacgc atccagttaa taaagcactc gtgaaatttt tcaggactga tacaggatac
    41761 aactctggca atggtatcgt gaacaggaat accattttca aaatcaccat attgcttcaa
    41821 aaaatcgaga tgagtttccc caaaatcctc tatatcttcc caaccttctg caccagaaat
    41881 aacggcacaa atagtcaaaa gtagaatatc tgacaattta tgctccactt tccaggcttg
    41941 tctgtaatcg gggataatag aaatatgttc catcaatttt ttaagtttca ttttgttctc
    42001 cttaattatg taaggagtat ttgatcatgt ataagcaatg gaaaacagct ttaggtaata
    42061 aggaatatct caattattga atatttagtg cgaattattt agtacaaaaa agcagcgttg
    42121 cgtgatcttt ccctgccctt tattcataaa cac
//
LOCUS       DAPOZO010000042        41875 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPOZO010000042 DAPOZO010000000
VERSION     DAPOZO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.042"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..755)
                     /locus_tag="ROI21_002308"
     CDS             complement(<1..755)
                     /locus_tag="ROI21_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDX5111222.1"
                     /translation="MNDRLCFEVHDNQGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWG"
     gene            complement(1166..2350)
                     /locus_tag="ROI21_002309"
     CDS             complement(1166..2350)
                     /locus_tag="ROI21_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDX5111223.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLQNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(2650..2744)
                     /locus_tag="ROI21_002310"
     tRNA            complement(2650..2744)
                     /locus_tag="ROI21_002310"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2708..2710),aa:Sec,seq:tca)
     gene            3037..4419
                     /locus_tag="ROI21_002311"
     CDS             3037..4419
                     /locus_tag="ROI21_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDX5111224.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQKVQN"
     gene            4429..6747
                     /gene="yicI"
                     /locus_tag="ROI21_002312"
     CDS             4429..6747
                     /gene="yicI"
                     /locus_tag="ROI21_002312"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDX5111225.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEEQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKHPDGSLWQWDKWQPGLAIYDFTNP
                     DTCKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKETVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNEQRPDYAW
                     HEGTAFQLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            7042..8595
                     /locus_tag="ROI21_002313"
     CDS             7042..8595
                     /locus_tag="ROI21_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDX5111226.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLEQWRITVEKRAGAGVMLDSKNITDLLHLDHLLGAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            8622..9092
                     /locus_tag="ROI21_002314"
     CDS             8622..9092
                     /locus_tag="ROI21_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004126743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDX5111227.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIAQLLVETSPQKIIDLFSQYAQSDVY"
     gene            9110..9424
                     /locus_tag="ROI21_002315"
     CDS             9110..9424
                     /locus_tag="ROI21_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDX5111228.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAAEISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            9449..10540
                     /locus_tag="ROI21_002316"
     CDS             9449..10540
                     /locus_tag="ROI21_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDX5111229.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            10552..11403
                     /locus_tag="ROI21_002317"
     CDS             10552..11403
                     /locus_tag="ROI21_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDX5111230.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINYMNLESARAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVTLALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTKAVVELAASAQLCVEGELGHLADAPRYDQAANADMMTQPTDVEA
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            11484..12344
                     /locus_tag="ROI21_002318"
     CDS             11484..12344
                     /locus_tag="ROI21_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDX5111231.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            12392..13324
                     /locus_tag="ROI21_002319"
     CDS             12392..13324
                     /locus_tag="ROI21_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDX5111232.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VAFIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITALNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            13305..13985
                     /locus_tag="ROI21_002320"
     CDS             13305..13985
                     /locus_tag="ROI21_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HDX5111233.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQLERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(14029..15738)
                     /locus_tag="ROI21_002321"
     CDS             complement(14029..15738)
                     /locus_tag="ROI21_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDX5111234.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRLADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     LLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(15859..17250)
                     /gene="xanP"
                     /locus_tag="ROI21_002322"
     CDS             complement(15859..17250)
                     /gene="xanP"
                     /locus_tag="ROI21_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDX5111235.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            17529..18734
                     /gene="gltS"
                     /locus_tag="ROI21_002323"
     CDS             17529..18734
                     /gene="gltS"
                     /locus_tag="ROI21_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDX5111236.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(18768..20849)
                     /gene="recG"
                     /locus_tag="ROI21_002324"
     CDS             complement(18768..20849)
                     /gene="recG"
                     /locus_tag="ROI21_002324"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDX5111237.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     AAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRSDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(20855..21544)
                     /gene="trmH"
                     /locus_tag="ROI21_002325"
     CDS             complement(20855..21544)
                     /gene="trmH"
                     /locus_tag="ROI21_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDX5111238.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(21551..23659)
                     /gene="spoT"
                     /locus_tag="ROI21_002326"
     CDS             complement(21551..23659)
                     /gene="spoT"
                     /locus_tag="ROI21_002326"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDX5111239.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(23678..23953)
                     /gene="rpoZ"
                     /locus_tag="ROI21_002327"
     CDS             complement(23678..23953)
                     /gene="rpoZ"
                     /locus_tag="ROI21_002327"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDX5111240.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(24008..24631)
                     /gene="gmk"
                     /locus_tag="ROI21_002328"
     CDS             complement(24008..24631)
                     /gene="gmk"
                     /locus_tag="ROI21_002328"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDX5111241.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFNTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            24889..26571
                     /gene="ligB"
                     /locus_tag="ROI21_002329"
     CDS             24889..26571
                     /gene="ligB"
                     /locus_tag="ROI21_002329"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDX5111242.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMMPPLNGAVIHPVAHTGVRK
                     MADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLKREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVARVRNAWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVVEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVFGLDGIGEAGWRALHQTHRFEHIFS
                     WFLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLLSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(26568..27185)
                     /locus_tag="ROI21_002330"
     CDS             complement(26568..27185)
                     /locus_tag="ROI21_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDX5111243.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(27478..28302)
                     /gene="dinD"
                     /locus_tag="ROI21_002331"
     CDS             complement(27478..28302)
                     /gene="dinD"
                     /locus_tag="ROI21_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDX5111244.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACLNSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(28524..29387)
                     /locus_tag="ROI21_002332"
     CDS             complement(28524..29387)
                     /locus_tag="ROI21_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDX5111245.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            29514..30230
                     /gene="rph"
                     /locus_tag="ROI21_002333"
     CDS             29514..30230
                     /gene="rph"
                     /locus_tag="ROI21_002333"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDX5111246.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            30296..30937
                     /gene="pyrE"
                     /locus_tag="ROI21_002334"
     CDS             30296..30937
                     /gene="pyrE"
                     /locus_tag="ROI21_002334"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDX5111247.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(30974..31570)
                     /gene="slmA"
                     /locus_tag="ROI21_002335"
     CDS             complement(30974..31570)
                     /gene="slmA"
                     /locus_tag="ROI21_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDX5111248.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(31677..32135)
                     /gene="dut"
                     /locus_tag="ROI21_002336"
     CDS             complement(31677..32135)
                     /gene="dut"
                     /locus_tag="ROI21_002336"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDX5111249.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(32113..33333)
                     /gene="coaBC"
                     /locus_tag="ROI21_002337"
     CDS             complement(32113..33333)
                     /gene="coaBC"
                     /locus_tag="ROI21_002337"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDX5111250.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAYFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            33505..34173
                     /gene="radC"
                     /locus_tag="ROI21_002338"
     CDS             33505..34173
                     /gene="radC"
                     /locus_tag="ROI21_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDX5111251.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSCVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            34390..34626
                     /gene="rpmB"
                     /locus_tag="ROI21_002339"
     CDS             34390..34626
                     /gene="rpmB"
                     /locus_tag="ROI21_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDX5111252.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            34647..34814
                     /gene="rpmG"
                     /locus_tag="ROI21_002340"
     CDS             34647..34814
                     /gene="rpmG"
                     /locus_tag="ROI21_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDX5111253.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            34912..35721
                     /gene="mutM"
                     /locus_tag="ROI21_002341"
     CDS             34912..35721
                     /gene="mutM"
                     /locus_tag="ROI21_002341"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDX5111254.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCEKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(35760..36239)
                     /gene="coaD"
                     /locus_tag="ROI21_002342"
     CDS             complement(35760..36239)
                     /gene="coaD"
                     /locus_tag="ROI21_002342"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDX5111255.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(36247..37524)
                     /gene="waaA"
                     /locus_tag="ROI21_002343"
     CDS             complement(36247..37524)
                     /gene="waaA"
                     /locus_tag="ROI21_002343"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDX5111256.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            37937..38995
                     /gene="rfaQ"
                     /locus_tag="ROI21_002344"
     CDS             37937..38995
                     /gene="rfaQ"
                     /locus_tag="ROI21_002344"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDX5111257.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHACGYEV
                     VLTSGPDKDDLVCVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDYIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            38992..40116
                     /locus_tag="ROI21_002345"
     CDS             38992..40116
                     /locus_tag="ROI21_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDX5111258.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEVE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            40109..40906
                     /gene="rfaP"
                     /locus_tag="ROI21_002346"
     CDS             40109..40906
                     /gene="rfaP"
                     /locus_tag="ROI21_002346"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDX5111259.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDIIKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            40922..>41875
                     /gene="waaO"
                     /locus_tag="ROI21_002347"
                     /gene_synonym="rfaI"
     CDS             40922..>41875
                     /gene="waaO"
                     /locus_tag="ROI21_002347"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HDX5111260.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVSYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDAIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQ"
BASE COUNT        10128 a        10101 c        10988 g        10658 t
ORIGIN      
        1 ccccataatg gtgagtatgt tgcatagtaa tcttctgccg tatccagaga gccggagaag
       61 tcatgccaca cagcaagtgg aaatggatgc agatttccgc acagcatttc tgcgatgtaa
      121 gtgtttgtgg caaagccatg gcgaaaagct gtggcggcct taacatgttc cccgttcaga
      181 aaatgcagga gaccgagttc ataccagtaa ggtgaaaatt catcagcatg ctctttcagt
      241 acactgaagg ctcgctcatg atcgccggtg cgtaacagct ctgaacccaa caaccagcga
      301 gccccctgat tatcttcagg attgtatgcc agtattttat cagtgagcat aaccgcatcc
      361 tgatggcgct gtaagtgcac atttgcgaga attgctgcat aaagcgcgcg cagataaggc
      421 cggttttcgg gatgcatcca gattatttca ccacagaaac tttccgggat gatacgatta
      481 cctgccgcca gtcctttaag agcggcgttc agtgccttac gcggggcatt ctgttccaga
      541 aatgcatatg ccagatgagc gtgtatatca ataaagtccg gctcccgttg agcaaggcgt
      601 cggagtttgt tgatgtaact cgtctcgctg atttcatcag cgtcgtatgc atcaagcact
      661 tcttcaaact cccccagcaa tgggccaaac caggtatccg gaaatacgaa ataaccctgg
      721 ttatcgtgca cttcaaagca taatctatcg ttcattttct gagcctgtaa aacatgtggt
      781 gatatagtct gagagcataa aagttgagat tctgtgtatc aagtcctgtc ttggcactga
      841 atgaatattg cttacaaagc cccttcatgc tttccttgag aacaataatt ttatctttat
      901 gtgaatctca ttttttataa tgatgtcaga tgattgaaat gctaaattat atctgaatca
      961 acttttcctt tgctaaaggt atcgaaataa ctgcaaggct gtattttgta attgccaatt
     1021 taaaaatgtc aatttgcttt taattatgtt tttgtaataa gtttgaagac gcaaaaaata
     1081 atataataac aaaaatcctc cagccttgta ggccagagga taaaatactt aattgaggaa
     1141 aacatcaggg aaaagtattt ttgacttaac tttcccggct ggagagtgaa tcaatgtaat
     1201 cagcgtacca ttgcatcatt tcacggcggc catcgagata cagggcatga ttataggtac
     1261 ccctgatgga atttttatcc acatgagcta actgcatttc aatccaggcg gaattaaagc
     1321 cttgttcatg cagaattgta ctcatggtgt gtctgaaacc atgccctgtg agtcttccct
     1381 tatatccaag taattcaatt acttggttaa tgctttcctt actaattggt ttgcggggat
     1441 catttctacc gataaagact aggggatgat gttttgagat tggttcaagc tgcttaaata
     1501 gcataatgac ctgctcagag agcggcacaa tatgaggtcg cttcatcttc attacttctg
     1561 ccgggatctc ccacaatttt gtctcaaaat cgatatcttc ccagcgtgca aaacgcagtt
     1621 cttgtgttcg tacgccggtt aacataatga tctgagtagc tgtcttagtg attatacttc
     1681 cggtataacc cgccagatcg ttgaggaagt ggggaagttc attagcagta agaaagggaa
     1741 aatgtacttt tttcggtgta gctaaagcac tggcaagatc gggggctggg ttatagtctg
     1801 cccggccagt aacaattgca tagcggaaca cctcaccaca gcgctggcgg accttgcgca
     1861 ttttctccag agctccacgt ttttccattt tacgcagtgc ttccagtaac tccattggtt
     1921 tgatttcggc aatagggcgc ttgccaatgt agggaaaaat gtctttttca aaggtatcaa
     1981 tgatttcatc acgatagcgt aatgaccacc gatcggcttt tgactggtgc cattcgcgag
     2041 caatagcctc gaacgtattt tgcagactca tttgctgcgc gattttttct tccttttttg
     2101 cctcccctgg atcgcctcca gcagcgagga gtttcctggc ttcactgcgt ttctcgcgag
     2161 catcggctag cgttacatcg gggtatacgc caatagaaag catcttttct ttaccagcaa
     2221 agcggtactt catgcgccag tatttagaac cattagtgtt aatcagtaga tacataccgc
     2281 cgccatcagc aagcttatag ggtttgtctt tgggttttgc ggttttcact ttgatatcgg
     2341 ttaatgccat gacatcctcc ggcttttgat gaattcatgt tcctgagggg gatttctgcc
     2401 agtaactaac ctgatacccc caatacaccc cttgtataac aaagatgttg ttaaaagccg
     2461 ttgttatata tcatattttt cctattatgg gggtgctggt atttcaatag gaaataacac
     2521 cccctaaagt acccccaaaa gaaacttgat acggggtgaa gtaggttgac ctctggatac
     2581 aaataaatac tgatatacaa gggattagaa gagatttatt gacattggga gagttcagaa
     2641 gaggtaaact ggcggaagat cacaggagtc gaacctgccc gggaccgctg gcggccccaa
     2701 ctggatttga agtccagccg cctcaccgga gacgacgatc ttccgcgcct cgattgctac
     2761 atggaggcgg ggcgcattat agctacttcc ttgagtttct acatccccca gatcagtttt
     2821 ttccgcctaa tacacgcagc gtattatcac gaaagcatta taattcatat aaaacccata
     2881 ttttacaatg atatatgagc ggaattaacg ctaccacgat cacataaaac aagaatcata
     2941 aattaataac cagatatcgg aatattcgct ctcacaggga tggtttacaa aatgcgttat
     3001 cggtctcagc acccctatag catcaaggaa aagcagatga agagtgaagt gttgtccgtt
     3061 aaagagaaaa ttggttatgg catgggagac gccgccagcc acattatttt cgataacgta
     3121 atgttatata tgatgttctt ttataccgat atttttggca ttcctgccgg atttgtcgga
     3181 accatgtttt tggtcgctcg tgcactggat gcgatttccg atccttgcat ggggttgttg
     3241 gccgatcgaa cgcgctctcg ctggggtaaa tttcgtccgt gggtactgtt tggcgcactg
     3301 ccatttggga tcgtctgtgt actggcctat agcacgccag atctcagtat gaacggaaaa
     3361 atgatttatg cagcaattac ttacacccta cttaccttac tttataccat cgttaatatc
     3421 ccttactgcg cattgggtgg tgtaatcacc aatgacccga ctcagcgtat ctcgctgcaa
     3481 tcctggcgtt ttgtgctggc gaccgcggga ggcatgctct ctactgtgct gatgatgccg
     3541 ctggttaatt taattggtgg tgataataaa ccactcggtt tccagggcgg tatcgcggtc
     3601 ctttccgtgg tggcatttat gatgctggca ttttgtttct tcaccactaa agaacgcgtt
     3661 gaagcaccac ctacaacgac gtctatgcgg gaagatttac gtgatatctg gcaaaacgac
     3721 cagtggcgta ttgtcggttt cctgaccatt ttcaatattc tggcggtatg cgtacgcggc
     3781 ggggcgatga tgtattacgt cacatggatt ttgggtacgc cggaagtgtt tgtcgctttt
     3841 cttaccactt attgcgtggg taacctgatt ggttccgcac tggcgaaacc gctgaccgac
     3901 tggaaatgta aagtcactat cttctggtgg acgaacgccc tgctggcagt gattagcctc
     3961 gcgatgttct ttgttcctat gcaggccagc atcactatgt ttgtcttcat cttcgtgatt
     4021 ggtgtgttgc atcaactggt gacacctatc cagtgggtaa tgatgtccga taccgtcgac
     4081 tacggcgagt ggtgcaatgg taaacgcctg accgggatca gttttgctgg cacgctgttt
     4141 gtgctcaaac tggggttggc cttcggcggc gctcttatcg gctggatgct ggcttatggc
     4201 ggatatgatg cggcagaaaa agcgcagaac agcgccacga ttagcatcat tatcgcgcta
     4261 ttcacgattg ttccggcgat ctgttatttg ctgagcgcga ttatcgctaa acgctactac
     4321 tcactcacga cgcacaatct gaaaaccgtt atggaacagc tggctcaggg caaacgccgt
     4381 tgccagcaac aattcacctc tcaaaaagtg cagaactaag gaacggcaat gaaaattagc
     4441 gacggaaact ggttgattca acctggcctc aatttgattc acccgcttca ggtgttcgag
     4501 gttgaagagc agggtaatga aatggtggtc tatgctgccc ctcgtgatgt gcgtgaacgt
     4561 acctggcagc ttgatacgcc tttatttacg ctgcgctttt tctccccaca ggaaggtatt
     4621 gtcggtgtac ggattgagca ttttcagggg gcgctgaata acggtcctca ttacccgctc
     4681 aatattttgc aggacgtgaa ggtcacaatc gaaaacacag aacgttacgc tgagtttaaa
     4741 agtggcaact taagtgcgcg tgtcagcaaa ggtgagttct ggtcactgga ttttctgcgt
     4801 aacggcgaac gtattaccgg tagtcaggtg aaaaataatg gctacgtgca ggacacgaat
     4861 aatcaacgaa attatatgtt tgagcggctg gatcttggcg ttggcgaaac agtttacggt
     4921 ctgggagagc gctttactgc gctggtgcgc aatggccaga cggtagagac ctggaaccgg
     4981 gacggcggca caagtactga acaggcgtat aaaaatattc cgttctatat gactaaccgt
     5041 ggttatgggg tactggtcaa tcatcctcaa tgcgtctctt ttgaagtggg atcggagaaa
     5101 gtctccaaag tgcagttcag cgttgagagt gaatatctcg aatactttgt tatcgacggc
     5161 ccgacgccaa aagcggtact tgatcgttat acccgtttta ctggtcgtcc ggcgctgccg
     5221 cccgcgtggt ccttcggcct gtggctaacc acttcattta ccaccaacta cgacgaagcg
     5281 acggtaaaca gctttatcga tggtatggcg gaacgcaatc tgccactgca tgttttccac
     5341 tttgactgtt tctggatgaa agccttccag tggtgcgatt ttgagtggga cccgctgact
     5401 ttcccggacc cggaagggat gatccgccgc ctgaaagcga aagggctaaa aatctgcgtc
     5461 tggattaacc cctatatcgg gcaaaaatcc cctgtcttta aagagttaca agagaaaggc
     5521 tatttactca aacacccgga cggttcgtta tggcagtggg ataaatggca gccaggtctg
     5581 gcgatttatg actttaccaa tccggatacc tgcaaatggt acgccgacaa actgaaaggt
     5641 ctggtcgcga tgggcgttga ttgctttaag accgactttg gcgaacgtat tccaaccgat
     5701 gttcaatggt ttgacggttc cgatccgcag aaaatgcata accattatgc gtacatctac
     5761 aacgaactgg tgtggaacgt gctcaaggaa accgttggcg aggaagaagc cgtcttgttt
     5821 gctcgctctg cctccgttgg tgcgcagaaa ttcccggtac actggggtgg cgactgttac
     5881 gctaactacg aatcaatggc ggaaagcctg cgcggtggtt tgtctattgg cctttcaggt
     5941 tttggcttct ggagccacga tatcggcggc tttgagaata ccgctccggc gcacgtttac
     6001 aaacgctggt gcgcgtttgg tttgctctcc agccatagcc gtttacatgg cagcaaatct
     6061 tatcgtgtgc cgtgggccta cgatgatgag tcctgtgatg tggtgcgctt cttcacgcaa
     6121 ctgaaatgcc gcatgatgcc ttatctgtat cgtgaagctg ctcgtgcgaa cgcgcggggt
     6181 acgccgatga tgcgggccat gatgatggag tttccggacg atccggcttg tgattacctt
     6241 gaccgtcaat acatgttagg cgacaacgtg atggttgcgc ctgtgttcac tgaagcgggc
     6301 gatgtgcagt tctacttgcc tgaaggtcgc tggacacacc tgtggcacaa cgatgaactc
     6361 gatggtagtc gctggcataa acagcagcac agcttcctga gtctgcccgt ttatgtgcgt
     6421 gataacaccc tactggcgct gggcaacaac gagcaacgtc ccgattacgc gtggcacgaa
     6481 ggcacggcat tccagctctt taatctacaa gacgggcatg aagccatctg tgaagtgccc
     6541 gctgctgacg gttccgttct tttcaccctg aaagcggcgc gtactggcaa cacgattact
     6601 gtgactggtg cgggcgaggc gaagaactgg acactgtgct tgcgcaatat tgtgaaagta
     6661 aatggtctgc aaggtggttc gcaggctgaa agtgagcagg gactggtggt gactcctcaa
     6721 gggaatgcgc tgacaattac gttgtaatct attgaagaat gccggatacg aatggttttg
     6781 tgtcgtatcc ggtaaataat ctcacatctg gtaataaata tttcttccat cgtatttgcg
     6841 agcatgatca cgattattca tcacgaaaat gtgatctgtg ccgaaaaata tctattaacc
     6901 gccccgtttt gttttataca tgtccggact acggacagat tttagtattg ttgtgaaatt
     6961 attctgcttt atatttaccc tcagagatgg caactgaagc tcttccacat ggggcttctt
     7021 aatctgtgat aagggtaaaa aatgcaaatg attacctcgc gacaaaacag attattacga
     7081 ttccttttac cacgaaggga atatacgact attgttacga ttgccggcta tttaaatgtt
     7141 tcggaaaaaa ccattcaacg tgatttacgt ttacttgagc aatggctgga acaatggcga
     7201 ataaccgttg agaagcgtgc tggcgcaggt gtgatgttag actcgaagaa tattactgat
     7261 ttgctgcatc ttgatcattt gttgggagca gaatgtgaag agattgatgg tgtaatgaat
     7321 aatgccaggc gcgttaaaat agcgtcgcag ttattaagtg aaacaccgaa tgaaacgtcg
     7381 atcagtaaat tgtcagagcg ctattttatc agtggagcct ctattgttaa tgacctgaga
     7441 gtgattgagt cctggcttgc gccgttgggg ttatcattga tccgcagccc aagtggtacg
     7501 catattgaag gtagtgaagg gcaagtccga caggcaatgg cattactgat taacggcatt
     7561 attaaccata atgagccgca aggtgtcgtg tattcacgtc tggatcccgg aagttataaa
     7621 gcattagtcc attattttgg agaagaagag gtgttatttg tccagtcatt gttactggat
     7681 atggaaaatg aattaagttg gtctttggga gagccttatt acgttaacat ttttactcac
     7741 atccttatta tgatgtatcg caacacgcac gggaatgcgt tatccagaga agaagatcaa
     7801 accaggcaat atgatgaaaa tatctttaat gttgccagtc agatgattca taagatagaa
     7861 caacgaattg cacatacatt gcccgatgat gaagtctggt ttatttatca atatatcatt
     7921 tcatcaggtg tggcgattga tggacaaaaa gatgttagca ttatttcaca tatgcaggcc
     7981 agcaatgaag cgcgtctgat tacctggcgt ttaattacgg tattcagtga catcgtggac
     8041 tgcgatttta gtgaagacag cgcattatat gatggcttgt tggttcatat taagccgctg
     8101 attaaccgac taaattatcg cattcatatc cgtaatccat tgctggaaga tattaaagca
     8161 gaattagcgg atgtctggcg gttgacgcaa tatgtggtga atcaggtatt taaaacctgg
     8221 ggtgagaatg cagtgagcga ggatgaagta ggttacctga ccgttcattt tcaggctgcg
     8281 atggagcggc aaattgcccg taaacgtgta ttactggtct gttcaaccgg aatcggaact
     8341 tcgcatctac tgaaaagccg tattctgcga gcatttcctg aatggacgat tgttgatgtt
     8401 atttcagcag cgaatttatc acaggttttg cctgacaata tcgaactgat tatttcgacc
     8461 attaatttgc ctacagtcac tatgccggtc gcttatgtta ccgctttttt taatgatgcc
     8521 gatattaagc gagtcactga aatggtgatt acggaaaaat tacatcatgc gacatctcgg
     8581 gtcgttgaaa tttaatgtca gaattttatg aggtatgacg aatggacata acaaaaatcc
     8641 tgaatacgaa tcgcgttatt ttagatatgc aagcgacgaa taaagcggag gcgattgaag
     8701 aattaactaa tcttctcaaa aaagatggtg cgatcgattg tcgggaaacc tttattaaag
     8761 atgtctggca gcgcgaagcg gaaggctcga ctggttttga aaatcatatt gcgatcccgc
     8821 atggtaaatc ttctgcggta attaatacca cgctggctat cgggcgtacc cggcaggata
     8881 ttccctggga aacactggat ggcagtagcg tacgctgtat tattttattt gcggtacgtc
     8941 ttgaagatca aaataccact catatccgtt tactttccca ggtcgcgggc gcgcttgcag
     9001 atgatgacat catagcgcaa ttgctggttg agacgagccc gcaaaaaatc attgatctct
     9061 ttagtcagta tgcacaatcc gatgtctact aaataacagg gagtatatca tgaatattgt
     9121 ctgtgttgcc gcctgcacgg cgggtattgc tcacacctat attgctcgtg aaaagttaat
     9181 taaaggggca aaagcactgg gccacaccat taaagttgaa acccagggaa ccattggtac
     9241 agaaaacgaa ttacaggcgg cagagatctc tgctgctgac gtcgtgattc ttgctgtgga
     9301 tgtgaaaata aaaggagaag aacgttttac caataaacgc attgtgcgag taaagacaga
     9361 gatcgtcatt aaatcgcccg ttcaatttct tgaaaaggtc gaaaaatcac tgggaaataa
     9421 atgatcctta tactggaggg atcttaatat gaaagaaaat aaaattccgg tttcacagga
     9481 aattaaaaaa catttattaa ccggcatctc gtggatgata ccccttattg tggccgccgg
     9541 gatatgtatt gcgttggggc aagttcttgg cggtacagat gttggtgaaa aaacaggaac
     9601 aatcccgtgg atgcttaatc agattggcgg ctggggaatg gggctgattg tgccgctgat
     9661 tagcgcggca attgcctatt ctattgccga tcgtcccgga tttgccccgg gcctgattgt
     9721 cggctttctc tgtgggcaaa tccataccgg atttattggc ggtatgttgg gtggtttcct
     9781 ggtgggctac accattttgc tacttaaacg ctatattcgc ctgccgcagt cgatgcaagg
     9841 actcatgcca atcatggtgt tgccggtatt aagcaccatt atcggtgggc tgttaatgat
     9901 gacgctgatt ggtaaaccca tcgcctggtt acaagaagca ctgattcatt tgctcgagtc
     9961 gatgcagggc ggatcgcgct tcctgatggg ggccatactt ggcgcgatgg caaccttcga
    10021 tttcggtggt ccggtcaaca aaaccatgtc gctgttttcc gatggcttgc tggtcagtgg
    10081 cgtctacgga ccagaagccg tgaaatttgt cggctcaatc atccctccgt ttggcatcac
    10141 actttcgttc ttactgacac gccataaata cacccgtgcg gagcgcgagg cgctgaaagc
    10201 cgcgtttccg atggggatct gcatgatcac tgaaggagtc attccgatcg ccgcgcgtga
    10261 cctgttgcga gtagtgggat cttgtgtggt ggcatcggcg gtcgcgggcg gcttaatcat
    10321 ggtgtggggc gtggagagtc cggttccaca tggcggcatg tttgttgtgc ctctgttcac
    10381 ccatcctctg ctgttttgcc tggcgctggg tataggcacg gtgatttgtg gcgtgatgct
    10441 ttcactctgg aagaaaccgg taacggaacg cgacgaagag tttgacgaac tgagcgatca
    10501 aaagctgaaa gatgaagaga tcactttcac cctcgaataa ccggaggccc tatgtttgcc
    10561 gatatgaaaa gtatggtgat gaaagcctgg catgaacatt acgccttgct ggcgatcaac
    10621 tatatgaatc tggaaagtgc tcgcgcagct atccgcgtgg cggagaagaa tcgcgctcca
    10681 atcattctca atctctatca ggggcatctg gcgcattttc ctgcacccgt cgcggcggca
    10741 gtggttaaaa cgctcgcgga atctgcctca gtaccggtca ctctggcgct tgatcatggc
    10801 aaaaatccgg actgtattcg tcaggcgttt cgtgctggtt tcagcggatt aatgattgat
    10861 gcttctgctt ttcccctgga ggaaaacgtg cgccagacca aggcggtggt ggaactcgcc
    10921 gccagcgcgc agctctgcgt tgaaggcgaa ctgggccatc tggcagatgc gccgcgttac
    10981 gaccaggccg ccaatgcaga catgatgacg caacccactg atgtggaagc gtttatcgcg
    11041 cagacaggca ttgatctact ggccgtctcg gtaggcacgg cgcacggcat gtacgcgcct
    11101 ggtgttgtac ctgctattga ttttcagcgt ctggcggaga ttttccactg ctcgagcgtg
    11161 cctctggcat tacatggtgg tagcggtacg ccgttcgatc agctccagct ctgtacgact
    11221 ctgggggtag caaaaatcaa cgtgggggcg gccatttttg aacgggggaa atccgcgctc
    11281 ctgcataccc tctgtcacga tatatccatt gaactggtgg acgcgctgaa agctatggaa
    11341 ctggccttcg aagaagcgat tacaccgtat ttacaggcga gcggctctat cggcaaagcc
    11401 tgattcattt tctgtcatgg cctttatgcc agtgcaataa cgcaccggca gggcggacta
    11461 cacgctgaaa ctggagattt aacatgttag tttcgatgaa agaattactg caacctaccc
    11521 gccagcacgg ctttgccatt ggcgcattta atgtggcgga taactgtttc cttcgcgcag
    11581 tcgtggatga agcagaagcg acaaatactc cggcgattat tgccatccat cccagcgaac
    11641 atgattttgt gggcgatgcc tttttcgctc acgttcgcga aattacccag cgtagtccgg
    11701 tcccgtttgt actgcacctc gatcatggtg cctctgtaga gcatgttttg cgtgcgattc
    11761 gctgcggatt tacctcagta atgatcgacg gttcgctgct gccgtatgaa gaaaacgtgg
    11821 cgctgaccgc agaagtggtt cgcctcgctc acgcggttgg agtgtcggtt gagggtgaac
    11881 tgggaaccat cggacaaaca ggaacgtcgg tagaaggtgg tgtttcacaa gtgacctaca
    11941 cagatccggc gcaggccgcc gattttattg ctcgtacagg tgccgacact ctggctgtgg
    12001 cgattggtac agcgcatggc atctatccaa aagggatgca gccgaagctg caaatggaca
    12061 ttttgcgcga cattgccgga cgtattgata ttccgctggt gctacacggt ggttcggcaa
    12121 acccggacgc ggagattgct gaatcagtta cgctcggcgt cggcaaaatc aatatctcca
    12181 gcgatatgaa atacgcctat ttccagaaag tgcgtgaaat tctcgctaaa gagagctggt
    12241 gggacccgaa cgtcatctat ccggatgcga ttaacgcggc gcgggaagtc attcgccaca
    12301 aaatgaaact gttcggttcg ctcggtaaag catcactgta ctaagccaga agggcggggt
    12361 gacccgcctt ttacgcaaca aggaacgaga tatgcactat ctggggttgg atattggtgg
    12421 gaccaaaatc gccgccgtcg tcatggatgc gcatggctgg gagattcgcc gttaccgctg
    12481 cccgacgcaa aagtcgacat atcaacaatt tgtctcatgc gttgtggcgt ttatcgagca
    12541 gattaggcgg gacgttcaac gaccgatgct gacagggatc gccttacccg gcagtatctc
    12601 gccactcact ggcctgatta aaaacgcgaa tattcaggtg attaacggtc atgcgttaca
    12661 ggctgatttg cagcaattgc ttgggcaacc ggtggtgata gccaatgatg gtaactgttt
    12721 cgcgctatca gaagcttgcg acggtgccgg gcaagattat gacgtggtat ttggtattac
    12781 gcttggtacg ggctgcggcg gtggcattgc catcaagcaa cgaccgttta taggggcctg
    12841 gggaaatgct gccgaatgcg gtcatatcac gttgccaggc tatacggagc aggaagatgg
    12901 tccatcagtc agttgctatt gcggcaaaca caactgcgtg gagtcgtttg tttccggcag
    12961 cggttttagt gaacgctatc aacagatgac tggtaacttg ctcactcctg cggcgattgt
    13021 caccctggca caacgtggtg atgcttgtgc catgcagcag gtggcacgtt ttcgccaaca
    13081 gcttgcccgc acgctggcaa ccatcgttaa cgttgttgac cctggcgtga ttgtgatcgg
    13141 cggcgggctt tcgaatgtgg aactgcttat caccgctctg aacgcagaag ttgctccact
    13201 ggttttcacc gatcaattca ccactcccat tgtaaaagca cagcacggcg acagtagcgg
    13261 aatgcgtggc gctgcctggc ttgctatgcg caacggagaa gccaatgaaa cgttcacaaa
    13321 ttaattatgt tattgataaa gcacatgcca ttgcgcagac cttccgcgtt tgtttgccag
    13381 agttcgccta ttttacggtg gatgactggc tgcagctgga acgggataac tggcaagaag
    13441 tggtcgactt acagctcggt tgggatatca ccgattttgg tcgtggtgat ttcaaccaaa
    13501 cgggactaac actgctaacg atgcgcaacg gcgcgctggg gtcggcggca tatccaaaac
    13561 cgtatgctga gaaaatgttg cagatccaac aagatcagca gactccctgg cattttcaca
    13621 cccacaaaat ggaagatatc ctcaatcggg gcggtggaga cttatgcatg cagttggcct
    13681 gggccaacga agccaatctc tgtgatgatc aacgagtcat taccgtgagt gttgatggtc
    13741 agcggcgtac catgaaacct ggtgaaacgc tggtgctaaa gccggggcag ggcatttgcc
    13801 tgccgccgcg tctgtatcat cgcttctggg cggaaaaagc gtttgttctc ggctgggaaa
    13861 tttcgatggt aaatgatgac caacacgata actattttct tgagccggga ggacgttttc
    13921 cggcgataga agaagatgaa ccggtgaaat ggttattgtg cagtgagtat ggaattttgc
    13981 gctaataacg gggaacgcta gcgctgatat ccgctagcgt ccttattttt actgcaccgg
    14041 ctccgtcgac gtaacttcac cactggaaac tacgcccgca ttcatggttt gagtcacttg
    14101 ctgctttgcg gcattcacgg catgcaattg cccgctaacc gtaggtttca gcgggacatt
    14161 ggcctgaata tcgccactgg cagtaagctg aatattgccg tcgccagtca acagtaattc
    14221 aggccatccc cactgttgca aaatattcac cggcacaccg cgtccattca ggctgatatg
    14281 ggtctgacgt tgtggcgttt gtgaaacact agcggtggct tccagaatgc ctttttcagt
    14341 aaatgcactc agttcgctga tattcaccgt gctgctgttg gcggtcagcg ccagcgatgg
    14401 gcgacgaaca tcaacacgat tgaatgtcgc ggcggcggca ttcagattcg ccgaaccact
    14461 ccagacgccc catttatgat cggtaaccag cgtcaggttg gcaccgtaac catcgagcgc
    14521 ggtgagctgc cacgggaagt cagggtcgat atcaatgatc agattgcggc tggcgctaaa
    14581 tctcttcagt tgcaggctgt ttaaccagcc gggtgtcgtt tccatccaca actgttgcca
    14641 gtttttcggc aaggtatatt ccagcccggc aattgccgca tcatcaagga tcaacgtttt
    14701 cccgtcacgc agccagttcc ctgacgttct aaccatgccc ccttcccagc ggctggtgaa
    14761 ctggcgcagc gctacgccct gcggggaaaa ttccgcgttt ataatcgggt caaataaatg
    14821 cagcgaacca taaatgaact cgctggcatt catcgacagt ttgccttcct gtgtctgcca
    14881 gtcatcttta ctgaaggtca tgttgcgcaa gctgagatcg aggtcggtca ccgcccagtc
    14941 cggaccttgc aaacgagcat cgatgacttc caggcgacca atctgcaacg acgggacaga
    15001 gcgtaatggc gcaaagaagt cggttagcga tttttcgctt tgtagacgga tatccgccag
    15061 ccgcagattt tccacttgcc agctgccgtc ggcgttacgc tgcgcgtttc cggttaatgt
    15121 cccgcgggcg atgtcggcac ccaggttagt cagcgtaacg cgatcgttat caatactgcc
    15181 ttcaatcagt acattggtgg caggaacatc attgagagaa agcgatccgg cactaaactg
    15241 aatctgcgcc ttcgtaccca gcactttacc ggcttctggt gaccacggaa ccacgccacc
    15301 atttacccgc tgcgcgctta gtttccattc gctattcggg ctattaaacg ccatatcacg
    15361 cagttgcaga cgatcggctt tgaacggtag cggcgcggtc tggtcggtga gattcagcgt
    15421 cccgttttcc agcaggatgg tatcgacatg gcgtggttcg gtcagttgcc gactgcttag
    15481 cgcaatgtcg acactttttg ccaccagggt cgcgggctga ccatcacgac caaacgtgac
    15541 gttctccagc acgatatgag atggcgcgga aaaacggtga tccatcgccc cgaaggccag
    15601 atgatagtcg ctattctcgg aaacccatgc gctgatatgt tctgctcccc agcgggtttg
    15661 cagaagaaaa tagaggccag cgatcaccac taacagagcg atgagaatgt agagaagcag
    15721 cttcccaata aatttcatgg tcttccatcc cgtgaaaagc acataaggga gttatgcacg
    15781 atttacgcgc aatcctcaag gcgggaatgg tgaaagacgt gagactgcgg cggtaattat
    15841 cttaccgccg ccagtgaatt actgtttttc tggtgggaaa atcagattca gcacgatggc
    15901 agtaataccg cccgcggcaa tcccggagga gagcaggttt ttcagccatt caggggcaaa
    15961 ctgcaaaatc agcggctgct gagagacgcc cagaccgacc gccagcgaca gcgcgataat
    16021 cagaatcgcc cgacggttca gcggctcacg agaaacgata cgcacaccgg aggcggcgat
    16081 ggtgccaaac attacaagcg ttgcgccgcc cagaaccggt tcaggaatgt gttgtacaaa
    16141 accgctcact gccgggaaca gacccagcac gatcaacatc agcgcgacga caaaaccgac
    16201 atagcggctg gcaacaccgg ttaactggat cactccgttg ttctgcccga agcaggagtt
    16261 cgggaaggtg ttaaacaccg ccgaaacaaa cgagttcagg ccgtttgcca gcacgccgcc
    16321 tttcaggcgt ttcatgtaca gcggaccgga cactggctgt tcggaaacgt cagaggtcgc
    16381 cgtgatatcg ccaatggttt ccagcgaagt gatcataaag accagcatca gcggcagcag
    16441 cagactccat tcaatgccaa gaccgtaata gagcggcgtt ggcaccataa tcagttcttg
    16501 tgtcatcggt tcgttgcttt ctggcaacat gcccataaac cacgccagcg catatccggc
    16561 cgccatcgca atcaccagtg aggccacgcg caaatatggg ttacgctgac ggttaagcag
    16621 gataattaag gctaagacca cgcccgccag cagcagattt ttcggtgcgc cgaaggtgtt
    16681 atcgctcatg gctgcgtaac cgccgccaat ggaggtcagc cccacctgaa ttagcgacag
    16741 gccgataatc atcaccacaa cgccagaaac cagcggcgta ataatgcggc gcgccagatg
    16801 cagaacgcgg gagatcacca tctcggtgca acttgccagc atcaacgtgc caaacaaagc
    16861 cgccatcatg gtaggaacat cagcaccacc ggttttcagc gcggtaccgc ccataatcag
    16921 cggggcaaca aagttgaagc tggtaccctg aatagacaac agcccggagc caaccggacc
    16981 ccaggcctta atttgaataa tcgatgccac accggaggca aacagcgaca tactaataat
    17041 gtgttgcgtg tcttgtgccg gtaaacccag cgcctggcag attaatagcg ctggcgtgat
    17101 caccgcaacg aacatcgcca gcaaatgctg acaggcggca aacagggttt gaggaagcgg
    17161 cggacgatct tcaagacggt aaatcagttc gctgttttga gtctgcgcaa ccggttgcgc
    17221 attttctgac tcgagggtgg aaacagacat cgaaaacaat cccgtggtag caaagcgggc
    17281 attttagctg accgaaagag aaaagcaaac gtttgccaca tcctcatcta aatatttaaa
    17341 tgagataaat gcactttttt atataacttt tggtattttt ctgcttaaaa tccatgccat
    17401 ttgttcatta acagctgaga atttgaccca ggaaagcata actcgatacc cgtcatagtt
    17461 cataagttgc cactgcgcga tgaagtatga cgagtatgaa agagtgatgc ggatacaaag
    17521 gagtaactat gtttcatctc gatactttag caacgcttgt tgccgcaacg ctgacgttgc
    17581 tgctcgggcg taagttggtc cattccgtct cctttttgaa gaaatacacc ataccggaac
    17641 ctgttgcggg tggtttgttg gtggcgctgg cgctactggt actgaaaaaa agcatgggct
    17701 gggaagtcaa ctttgatatg tccctgcgcg atccgttaat gctggcattc ttcgccacca
    17761 ttggcctgaa cgccaacatt gccagtttgc gtgccggtgg gcgtgtggtt ggcatcttct
    17821 tgattgtggt tgttggtctg ttggtgatgc aaaatgccat tggcattggt atggctagcc
    17881 tgttagggct tgatccgctg atggggctgt tggccggttc tattacgctt tccggcggtc
    17941 acggtacggg cgctgcgtgg agtaaattgt tcattgaacg ctatggcttc accaatgcga
    18001 cggaagtggc gatggcctgt gcgacgtttg gtctggtgct gggcggcttg attggcggtc
    18061 cggtagcgcg ctatctggtg aaacactcca ccacgccgaa cggtattccg gatgaccagg
    18121 aagtcccgac ggcgttcgaa aagccggatg tgggccgcat gatcacctcg ctggtgctga
    18181 ttgaaactat tgcgctgatt gctatctgcc tgacggtggg gaaaattgtt gcgcaacttt
    18241 tggctggcac tgcttttgaa ctgccgacct tcgtctgtgt actgtttgtt ggcgtgattc
    18301 tgagcaacgg tctgtcaatg atgggctttt accgcgtctt tgagcgtgcg gtatccgtgc
    18361 tgggtaacgt aagcctgtcg ttgttcctgg cgatggcgct gatggggctg aaactatggg
    18421 agctggcttc gctggcgctg ccgatgctgg cgattctggt ggtacagact atcttcatgg
    18481 cgttgtatgc catcttcgtt acctggcgca tgatgggcaa aaactacgat gcggcagtgc
    18541 tggctgcggg tcactgtggt tttggcctcg gtgcaacgcc aacagcaatc gccaacatgc
    18601 aggcgatcac tgaacgtttt ggcccgtcgc acatggcgtt cctggtcgtg ccgatggtcg
    18661 gtgcgttctt tattgatatc gttaacgcgc tggtgattaa gctgtattta atgttgccga
    18721 tttttgcggg ctaaccgatg aagcggcggt cttctcaccg ccgcctttta cgcattcgag
    18781 taacgttccg tctccggcat ccagcgttct atcagggctt ttgcctgttg tgggtaacgt
    18841 tcgtgaatat gtcgcgctag gcgctgaact tccgggatca tcgcctgatc gcgcagtaaa
    18901 tccgccactt taaactcagc attacccgtc tgacgcgtac ctaacagttc acccggtccg
    18961 cgaatctcta agtctttctg tgcaatcaca aaaccgtcgt tgctgtcgcg cagtacctgc
    19021 aggcgaattt gcgccgtttt agaaagcggc gttttgtaga gcagcacgca gtgagaagcc
    19081 accgcaccac gtcccacgcg cccgcgaagc tggtgcaact gcgccagacc cagacgctcc
    19141 gggttttcga taatcatcag gctggcgtta ggcacatcaa cgccgacttc aataacggta
    19201 gtggcaacca gcaggtgtaa ctcgccttgt ttaaatgatg ccatgacggc ctgcttctcg
    19261 gctggtttca tccgtccgtg taccagacca acattcaact ctggtagcgc cagtttcaac
    19321 tcttcccagg tagcttccgc cgcctgcgct tccagtaact ctgactcttc aatcaacgta
    19381 caaacccagt acgcctgccg accttcagtc atgcaggcgt ggcgcacgcg gtcaatgatg
    19441 tcgctacggc gagtatcagg aatagcgacc gtagtcactg gcgtgcggcc tggcggcagc
    19501 tcatctatca ctgaggtatc gagatcagca tacgcagtca ttgccagcgt tcgagggatc
    19561 ggcgtggcgg tcatgatcaa ctgatgagga tggaagcctt gctgctggcc tttctcccat
    19621 aacgccagac gctgatgcac gccaaaacga tgctgttcgt cgataatcac cagcgccagg
    19681 ccgttaaact gcacctgttc ctggaagatg gcgtgcgtac caacaatcat ctgcacctga
    19741 ccgctggcaa tggcttcctg ctgtgccagc cgcgctttac ctttctgctt accggcgagc
    19801 cagcccactt cgataccgag cggttcaaac cagttgcgga agttattggc gtgctgttcg
    19861 gcgagtaatt cggttggtgc catcaatgcc acctgtttgc cgtgggcaat cgcacgcagc
    19921 gcggcgaggg cggcgaccag cgttttaccg gaacctacat cgccctgtac caggcgcatc
    19981 atcggcacat ccagcgccat atcgcgctcg atctccgccg ctacgcgtgc ctgtgcgccc
    20041 gttggcttga acggtaaggc tgcgaggagt ttatttttca gcgcgtcatt ggcgctcagt
    20101 ggctgggcat gaaaacgctg tgctccggca cgtaaggcta acatgctgag gttgtgcgcc
    20161 agcagttctt ccagaatcag acggcgttgt gccggatgct ggccggtttc cagatcgcta
    20221 agctgtagcg tcggcggcgg gcggtgcaaa gtgcgcaacg cttccggtag cgtcatcatt
    20281 ccttgtgaca gttccggcgg taggagttct tcaatggcgc aggtgtcgag cagatccagc
    20341 gcctggtcgg ttaatttacg cagcgtggcc tgctttacgc cttcggttgt tggataaacc
    20401 ggcgtaagcg tttcctgcaa ctctggcgta ctgagatcgc cttgcacgcg gtattccggg
    20461 tggatcatct ccgcgccata tttaccgcgc tttgcttctc cataggcgag cacgcggcgg
    20521 cccgtcgcca ggctattttt cattgccgcg ttgaagttga aaaagcgcat ggtgagaatg
    20581 ccggaaccgt cgctgatctg gcaggtcatc atccgccgac cgccgaagga gatattgcag
    20641 ttcagcactt cgccttccac cgtggcataa acgcccggta gtagttctcc aatggggtag
    20701 agatgggtgc gatcttcgta gcgcagggga aggtgtaaga gtagatcctg cacggtatgc
    20761 aggttgattt tcgccagctt gttactaagc gctgcaccaa cgcccgttag ggaactgagc
    20821 gggacagcat ctaacaggcg acctttcatg gcacttatcc tgcagcctgc atggtagccc
    20881 accagtcggc atcggcttca atttcgccct gctgattgac gtgggggtaa ggcaggcctt
    20941 tgcgttttgc gactttcgcc agcaccggat agccgccttc aaacagcagg cgttgttgct
    21001 ctgcttccgg caacatgctg ttttcacgca ggtacatgcc cgcattttgc cgctggcgct
    21061 gggcttcgta aagaatgagg gctgaggcaa cggaaacatt cagcgattgc accatgccaa
    21121 tcatcggaat gatgatgtcc cgatccgcca gggccaacgc ttcctgcgtg atgcctgttt
    21181 tctcctgtcc catcaaaata caggtcgggc gagtgtaatc aatttcgcgg aaatcgacag
    21241 cgttatcaga aagatgggtt gccagaatct gcatgccctg gcctttcaga tgagcgacgg
    21301 catcgccaat ggtgcggtgc gttttcacct gtacccagct gttactaccc gcagctgccg
    21361 aagccatggt gcgcatccgg ctaccaggcc agacagcatg aacttcatgt acaccaacgg
    21421 catctgcggt acgaataatc gcagaaacgt tatgaggttt gtggacctgc tccatgcaga
    21481 cggtcagatc aggctgccgc ctggcgagca tttcgcagat acgtgcataa cgtgttgggt
    21541 tcataaaaca ttaatttcgg tttcgggtga ctttaatcac gtctggcatc acgcggattt
    21601 tgcgcatgat attcgccaga tgcacacggt cacgggcggt cagacgaata aaggcgctgt
    21661 agacgcgacc atctttctct tccgtattca aactttgaat attcgaggtg gtggtgttaa
    21721 ttgccgctgt caggtttgcc agcgcgccct gatgattgaa catctccacc ttgatttcgg
    21781 tgatgaactc ctgcgccgtc tctttatccc attccacagc cataaacttc tcaggctctt
    21841 tctggtagcc acggatgtta cggcaggatt catggtggat caccagacct ttaccggggc
    21901 tgacgtgggc gataatcggg tcgccaggaa taggacggca gcatttcgca aaggtgatca
    21961 gcacgccatc ggcaccttta atgggcagat gtccgtgact ttgggttgcc ggtggaatgg
    22021 aggcgtcccc atgttgcaga tttttcgcga ccaccacgct cattgcgtta ccaaggccga
    22081 tttcagccag cagatcgtca agcgttgcca gcttcatgcg gtccagctcg cgctgaatat
    22141 tttcctgcgg gatttcattg agcttgcggc tgccacccaa cgcatggttg agcagacgac
    22201 ggcccaggct tacagaatca tcacgcttga ggtttttcag caattgacga attttggcgc
    22261 gcgctttcga gctaacgaca aagttcagcc acgcggcatt cgggcgagcg ccaggtgcgg
    22321 taataatttc aacggtttga ccgctggtaa gcggctgcga cagcgggtaa ggctggcggt
    22381 cgacgcgtgc gcccacgcag gcatgaccga tatcggtatg caccgcataa gcgaagtcga
    22441 cgggcgttgc accggcaggc agctctacaa tgcgcccttc cggtgtgaaa acgtaaattt
    22501 catccgggaa gagatcggat ttaacgctct cgataaattc aaacgagcta ccggcactct
    22561 gttgcagctc cagcaggctt tgcatccagc gctgggcgcg gatttgtgcg gtagtactgg
    22621 tttcgccgtg ctctttataa gcccagtgcg cggcaacacc catctctgcc atctgatcca
    22681 tatcttcggt acggatctgg acctcaaccg gcacgccgtg cgggccaatc atcgaggtgt
    22741 gcaaagactg atagccgttc gcttttggaa tggcgatgta gtctttcacg cgacccggac
    22801 gcggcttgta caggctatgc atttgcccga gcacgcgata acaggtgtcg gaatcattga
    22861 cgatcacgcg gaaggcgtag atgtccatga ttgagtgaaa acgctgctct ttgagcacca
    22921 ttttgcagta gatcgaataa agatgctttt cgcgaccact gacgcggcac ggtattcccg
    22981 cttcctgcaa acgcccttcg atttcagaaa gaatcttctg gatcatctct ttacggttgc
    23041 cgcgcgcggc tttcaccact tctttgatta cgcgataacg gttgggatac agcgcctcaa
    23101 aacccagctc ttcgagttcg gttttaatgt ggtggatacc taaacggtgc gccagcgggc
    23161 tgtaaatttc gagtgtttca cgggcgatgc ggcgacgttt gtccgggcga agtgagccca
    23221 gcgtgcgcat gttgtgggta cggtcggcaa gtttgatgag aatgacgcgg atatcctgca
    23281 ccatcgccat aatcatcttg cgaaagtttt ctgcctgcgc ctctttctta tcgcggaact
    23341 tgagcttatc aagtttcgac accccctcta ccagctcggc gacgctttta ccaaaaagct
    23401 gttccatgtc ctggtaggtg gcgggagtat cttcaatcac gtcatgcagc agcgccgcca
    23461 ttagcgtttc atagtcgagt ttcatctcgg ccagaatgca ggcaactgcc accgggtgcg
    23521 tgatataggg ttcaccgctt gaacgtgttt gcccctcgtg agcatcacgt gcaacgagat
    23581 acgcctgccg cagacgcttg atttggtctt ccggcaggta gttttgaatc agctgattca
    23641 ggctttcaaa cagatacaag ggcgacccgc tttgtgatta acgacgacct tcagcaatag
    23701 cggtaacggc ttgtaattca gcggcttcct gctcttgctg ttcctggcgt tcgcgcacgt
    23761 cgaggatctg gttgttgatc agaccttctt cgatttcgcg cagcgcgatt acagtggttt
    23821 tatcgttttc ttccggtacc agcggatcct ttccgcctac ctgcatctga cgagcgcgac
    23881 gcgcggcgac cagtaccagg tcaaaacggt taccaatttt ctctacagcg tcctgaacag
    23941 ttacgcgtgc catacttaaa aagctccaca ggtgaagaaa tgactgggca tgatactgaa
    24001 atcaggttca gtctgccaac aatttgctga ttaaagcgtc atgacgctgc ttttggcggc
    24061 tcatgcgcag acgttcggcg cgaataatgg tcttcaaatc ggtcaacgcg gtattgaagt
    24121 catcattcac aatcaggtaa tcatattcgg cgtaatggct catttctgca acagcttgcg
    24181 ccatacgctt tgcaatgacc tcttcgctgt cctgaccgcg accgcgtaga cggcggtcca
    24241 gttcaatttt ggacggcggt aaaataaaga tactccgcgc gtgcggcatc ttctggcgaa
    24301 tttgctgcgc gccctgccag tcgatatcga gaaaaacatc gacaccggtc gccaggactt
    24361 gttcaatggc ctcacgcgaa gtgccatagt aattaccaaa aacttctgcg tgttcgagga
    24421 acgcatctct gctaatcatt tctttaaatt catcgtgatt aacaaagaaa taatgttcac
    24481 cgtggacttc accaggacgc ggttggcgtg tggtgtgtga aacagaaacc tgggtgtcat
    24541 acaacggttg ggtttttaat aaagcctgaa tcaggctgga tttacccgcg ccactggggg
    24601 cagaaacaat ataaagcgtg ccttgagcca tgagtatctt tcgtatgtga ttagcgaaat
    24661 aaagcctaca tacgagctta ttatacacgg cgcggccatg tgacgtagcc cttgctcaca
    24721 ctttttgcgc aaagttgttg cgttttgcgt agctgcttcc gcatttgcct gtcattcact
    24781 gcaataaaga cgattctcag gaagcgtcct cgcagactcc gtttttgcca ctcggctttg
    24841 ctttcatcac gttgtatatc tgcctgacta tctacagcgg aggtgatgat gaaagtatgg
    24901 atggcgatat taataagtat cttgtgctgg caatcatcgg cgtgggcggt ctgtccggcc
    24961 tggtcgccag ccagagcaca ggaagaaatt tcccgcctgc aacagcaaat aaaacagtgg
    25021 gacgatgact actggaagga agggaaaagt gaggttgaag acggtgttta cgatcagtta
    25081 agcgcccgtc ttacgcagtg gcaacgctgt tttgggaacg agacccgcga tgtgatgatg
    25141 ccgcctttaa acggtgcggt tatacatccg gtagcccata cgggtgtacg caaaatggcg
    25201 gataaaatcg cattaagtct gtggatgcgc gaacgtagcg atctttgggt gcagccaaaa
    25261 gttgatggcg ttgcggtaac cctagtttat cgggacggga aactgaacaa agcaatcagt
    25321 cgcggcaatg gcctgaaagg cgaggactgg acgcagaaag ttcgcttaat ttccgctgtg
    25381 ccgcaaaccg ttagcgggcc tttagccaac agtacgcttc agggggaaat atttctcaaa
    25441 cgcgaggggc atatccaaca acaaatgggg ggaataaatg cccgcgcaaa agttgctggt
    25501 ctgatgatgc gccaggacga tagcgacacg ctgaattctc tgggtgtttt tgtctgggca
    25561 tggccggatg gaccgcagtt aatgaccgat cgtttaaaag agctggctac cgctggtttt
    25621 actctaacgc agacgtatac ccgtgcggtg aaaaatgctg atgaagttgc gcgcgtacgc
    25681 aatgcgtggt ggaaagcgaa attacccttc gtcaccgatg gcgtggttgt gcgagcggct
    25741 aaggaacccg aatcccgtca ttggctaccg ggccaggcgg agtggctggt ggcctggaaa
    25801 tatcaaccgg tagctcaggt tgtcgaagtg aaggcgattc agtttgcggt gggtaagagc
    25861 ggtaaaatat cggtggttgc gtcactcgca cctgtcatgc tggatgataa aaaagtccag
    25921 cgggtgaata ttggttccgt caggcgctgg caggagtggg atattgcgcc tggtgatcag
    25981 atcctcgtca gccttgccgg tcagggtatt cctcgcattg atgatgtggt gtggcgcggt
    26041 gcagaacgta caaaaccgac accaccagaa aaccgcttta actcgttgac ctgctacttt
    26101 gcttctgatg tttgtcagga acagttcatt tcacgcttag tctggctggg gtcaaaacag
    26161 gtttttgggc tggatggcat tggtgaggcc ggctggcgcg cgctgcatca gactcatcgc
    26221 tttgagcata tcttttcctg gtttttatta acgccagagc aattacagaa cacgccgggg
    26281 atcgcgaaaa gtaaaagtgc gcagctatgg catcagttta atctggctcg taagcagcct
    26341 tttactcgct gggtgatggc aatgggaata ccgctaaccc gggcggcgct taatgccagt
    26401 gatgaacggt cctggtcgca acttttattg agcacggagc agttctggca gcagctgccg
    26461 gggactggat cggggcgcgc cagacaggtt attgaatgga aggaaaatgc gcaaatcaag
    26521 aagctgggca gttggctggc tgcccagcag atcacaggtt ttgaacctta gtgtccttcg
    26581 tggctgtagt aaaagaccgg taatcccagc ttaagacgta gcgccagtaa acgggcaaaa
    26641 aagccgaata ccagggtgga gataatcacc acatcatggt tagaaacata gtgttgcagc
    26701 gcgatgtaca gcacggcgga ggcaaatgag acaccggcat acaactcttt ctggaatacc
    26761 agtgggatgc gtttacagaa catatcgcgc aaaacgccgc caaatacgcc ggtcgtcaca
    26821 gctgcgacaa cggcaatgat cgggccgtgt cccatatcca gcgcaacctg tgcgccaatg
    26881 atagaaaaga ctaccagtcc gagcgcatcg agcaccagaa acactttgcg caggtaaggc
    26941 attacaggag caacaatagt ggtgagtact gcggcggtag cgacgataat cacatattcc
    27001 gggtgtttga cccagccgag cggatagtgg cccagcagaa tatcgcgcac tgaccctccg
    27061 ccaattgcgg tggcggtcgc aataataatt acgccaaatg tatccatgcg ccgtcgtccg
    27121 gccgccagcg cccccgtcat ggcttcggca gtaatgccaa ccaaatacaa aatgtgtaac
    27181 agcatggttc tctccctaaa aaatctgtct ggcagggtag cgatttgtgc gtattgtcac
    27241 gattgagatt ttttaaggtg agaggaaatt gataacttgt agttatgtga aatcgctatt
    27301 ttctgtagcg tagatgcatt cttctgactt cttgtctgag aaaatgcata agaaaaaata
    27361 ggttaaaatt gaggagtgga aaagaaaggt gcaatgttta gccattgtgt tcaccgcacc
    27421 tggactttgg cttacgcatc gggtatctca atcaacaaaa agagttttta gtttctgcta
    27481 tttcttctct gtaattttta cactgttttc caactgcttg atgctgacct gcggggtcgg
    27541 caactcctca ggcatggttc cgccaagttc ctgaatggtt tgcctcacct tgcgacccac
    27601 gtcaaagtgt gtggtgttag cttgctgttt tgaattcacg ccatcccgct tgagtttttc
    27661 ttctgtttgg gtagctcgaa agagattagc cgccagttct gttgaaccca tatgatccag
    27721 gatcttctga ctctttttca gccccttccg ctgatggatg gctttctgat ctaatccacc
    27781 atacagtccc cggtaaccat gattctgaaa tatggcgaaa tcgatagctg tcgctacccc
    27841 agcttgctgc gctgcctcaa ctaattgttt gttatgttct ttcaactcat ttctcagaaa
    27901 aagacgcttt tcgtcttcac gaagttgcct gaaagcttca tcgtcagcca gctcctgccg
    27961 tcgggtctgg atagcaaaat aagtttgccc tgccgcaata accggtttcg cagggtcgcc
    28021 gttttgtact accaggtaac aggcatagcg ggagagtacg atgtctttta atgctctttt
    28081 ggcatttgag ccaattttga ccatatcgag gatctcctcg aaatggtcat caattgtgtg
    28141 accactattc agacaagctt ctttggcgcg cgttaataca ggtataaagt gacgatactc
    28201 tgaataaccc agtagttttc caagttgtct tgctgaccat tgttctgctc cgtttgcatt
    28261 aatcagcttt atctcttcaa aaggttgatg atgttcgttc attgcccact ccatgtgata
    28321 tcgattgcaa ggaaagtaag tttacaactg tgtttatata cagttgtgag tatctcacgt
    28381 tctcactgcg ctgggcaaaa aaacatcgca aaatctggag caggcacgca aaacagaatt
    28441 gaatgttgcg gggaggaaaa atgccgctct gtttgagtgg gcggcatttg tgaaggctga
    28501 aaaggaagaa ttggcgttac gagttattca atgttctgga tctgctcgcg catctgctca
    28561 atcaacactt tcagctcgat ggcagaattc gtcacttcgg cattgataga tttcgacgca
    28621 agagtgttcg actcgcggtt gaactcctgc atcataaagt ccagacgacg accaaccgct
    28681 tcttttttct tcagaatgtt gtaggtctct ttgacatgcg cttcgaggcg gtccagttct
    28741 tcggcaacgt caattcgttg tgccagcaga accaactcct gctccagacg gttgttttcc
    28801 agttgcacct gagcatcttc cagcttcgcg accagacgct cacgttgcca ttgcaggatt
    28861 tccggcatat gggcgcggac tttgaccact tcggcggtga cgccttccag acgctgctcg
    28921 atcaatgctt tcagcgcctg accttcggtt tcacgtgcga cgataaagtc gtccagcgta
    28981 ccatccagcg ccgcgagaat ttcagcggca atcgcgtcga gatcctgctc ctgggctgcc
    29041 attacgcccg gccagcgcag aatatcaacc gggtttattt ccccttcgtc gctctgcatt
    29101 tttacccagt tcgcggcggt taccagctgt ttagccagtt tttcgttgag gatcagctca
    29161 ccttgtgcgc taacatctgg ttcataacgc agggtacatt ccactttacc gcgcgtcagg
    29221 cgagaacgaa tacgctcgcg aacgacaggt tcaaggctac ggaactgctc cggcagacga
    29281 aagtaagttt ccagataacg ctggtttacc gagcgcattt cccaggttgc gctcccccat
    29341 tcacccttga tttcacgccg ggcgtaggcg gtcatactgc ggatcataga cgttcctgtt
    29401 tataaaagga gaggtggaag gattatagcc atcgatgcct tgtcaggata ggaataaccg
    29461 ccggaagtcc gtataatgcg cagccacatt tgtttcaagc cggagatttc aatatgcgtc
    29521 cagcaggccg tagcaataat caggtgcgtc ccgttaccct gactcgtaac tatacaaaac
    29581 atgcagaagg ctcggtgctg gtcgaatttg gcgataccaa agtattgtgt accgcctcta
    29641 ttgaagaagg cgtgccgcgc ttcctgaaag gtcagggcca gggctggatc accgcagagt
    29701 acggcatgct gccacgttct acccacaccc gtaacgcgcg tgaagcggcg aaaggtaagc
    29761 agggtggacg caccatggaa atccagcgtc tgatcgcccg tgctcttcgt gcggcagtag
    29821 atttgaaagc gctgggtgag ttcactatta cgctggactg cgacgtgctt caggctgatg
    29881 gtggcacgcg taccgcttcg attacgggtg cctgcgtggc gctggcagat gcgctacaga
    29941 agctggtgga aaacggtaag ctgaaaacca atccgatgaa agggatggta gccgcggttt
    30001 ctgtcgggat tgtgaacggc gaagcggttt gcgatctgga atacgttgaa gactctgccg
    30061 cagagaccga catgaacgta gtgatgaccg aagacgggcg catcattgaa gtgcagggga
    30121 cggcagaagg cgagccgttc acccatgaag agttactcac cttgttggcg ctggcccgag
    30181 ggggaatcga atccattgta gcgacgcaga aggcggcgct ggcaaactga tttttaaggc
    30241 gactgatgag tcgccttttt tttgtctgta gaaaagtaag atgaggagcg aaggcatgaa
    30301 accatatcag cgccagttta ttgaatttgc gcttagcaag caggtgttaa agtttggcga
    30361 gtttacgctg aagtccgggc gcaaaagccc ctatttcttc aacgccgggc tgtttaatac
    30421 cgggcgcgat ctggcactgt taggccgttt ttacgctgaa gcattggtgg attccggcat
    30481 tgagttcgat ctgctgtttg gccctgctta caaagggatc ccgattgcca ccacaactgc
    30541 cgtggcgctg gcggagcatc atgacctgga cctgccgtac tgctttaacc gcaaagaggc
    30601 aaaagaccac ggtgaaggcg gcaatctggt tggtagcgcg ttacaaggac gcgtaatgct
    30661 ggtagatgat gtgatcaccg ccggaacggc gattcgtgag tcgatggaaa ttattcaggc
    30721 caatggcgcg acgcttgctg gcgtgttgat ttcgctcgat cgtcaggaac gggggcgcgg
    30781 cgagatttcg gcaattcagg aagttgagcg tgattacaac tgcaaagtga tctctatcat
    30841 caccctgaaa gacctgattg cttacctgga agagaagcca gaaatggcgg aacatctggc
    30901 ggcggttaag gcctatcgcg aagagtttgg cgtttaaaga aactcgccgg atgaaaagtc
    30961 atccggcgtc atattactgc aactgtgcgg caattagcgg ccagcgggcg tcaaaatcat
    31021 ccgtcgggcg gtatttaaac tcgctgcgga caaagcgtga cagcatacct tcacagaagg
    31081 ccaggatctg gcttgccagc agggtttcat cggtggtgta accttcaccc tcacgcattc
    31141 tcttttcgcg caatacctga cgcaactgcg cttcaatacg ctcgaacagc tggttgatgc
    31201 gcccttgcag gcgatcctgt tcaaacatta gtgcatgacc agtgaggatg cgggtcaggc
    31261 caggattacg ctcaccaaaa ccgagaagca gcaacacaat cagacgcagg cgcgctgtgg
    31321 tgtccttctc atctttcaga atcagattga tgcgagtaat caggctatct tcgataaact
    31381 caatcaggct atcgaacatg cgggtcttac tggggaagtg gcgatacagt gccgcttcgg
    31441 aaacgccgac agaggcggcc agttttgccg tcgtgatacg ttggcttcca tcgctggatt
    31501 ccagcatcag cgccagagac tgaagtattt cctcgcgacg gttccttttc gcagtttgtt
    31561 tttctgccat gttacaaaat acccctgaaa ataagcactt gccaggcggc acccacgcta
    31621 tgaccgcaaa cgaaatgttt gcggctatgt tatgacgtta ttcggatgcg tatgtgttac
    31681 tgacgaccag agtgaccaaa gccgccttca ccgcggtcgg tggcgtcgaa atcttccacc
    31741 agattaaatt cagcctgtac taccggaaca aaaatcatct gggcgatgcg ttcgccaggt
    31801 tgaatggtga agctgtcctg accacggttc cacacggaaa tcatcaactg gccctgatag
    31861 tcagaatcga tcaatcctac caggttacca agcacgatac cgtgcttatg tcccaatccg
    31921 gagcgcggca gcatcattgc cgccagtgaa ggatcggcaa tatgaatcgc cagcccggtc
    31981 ggaaccagcg tagtgtcacc cggagccagt tctacggcgt cgtcgagaca ggcacgcagg
    32041 tcaagtccgg cagagccaga ggtggcataa gtcgggagcg gaaattcctt cccaacgcgc
    32101 gggtccagaa tcttaacgtc gatttttttc atcataacgg gtcacgatct cgtcgagtaa
    32161 taattggcca aggagctctt tgcgctcaag cggtaagact ttatctccgt cctgccagaa
    32221 aaggtgtaat gcgttgttgt cgctgttaaa tccttgagtt ggctgggaaa catcgttcgc
    32281 gcagatcaga tcaaggtttt tacggatacg tttttgccgg gcgtattctt ccacattatt
    32341 tgtttcggcg gcaaatccaa cgacgtaggg tcgatggtct tttagtgcgg caacgcctgc
    32401 gacgatatcg gggtttttaa ccatttttat tgttaattca tcaccctgcg tggcctgttt
    32461 tttgattttc tctggggcca cggtagctgc gcgataatcc gccacggcgg cacagccgat
    32521 aaaaatattt tgctgctgta cagaagcatt cacggccgct tccatttcca gcgcggtcat
    32581 cacatcaaca cgtttaacaa acggtggcgt cggtagtgaa accggacctg ataccagcgt
    32641 gacgttcgcg ccacgacggg cagcggcggc ggcgatagca aaacccatct tgcctgagct
    32701 gtgattagag atataacgca ccggatcgag cggttcacgc gtcgggccgg cggtaatcat
    32761 aatgttcaga tgtttcaggt cgttgacggg cgaaaaatac gctaccgcca tatcaacaat
    32821 ggttaacgga tcgagcattc gcccagggcc gatatcacca caagcctgac tgccactgtc
    32881 tggcccccag atgagcaaac cacgggaagc aagcacctct aaattatgct gcgtggcagc
    32941 ggcacggtac atctgctggt tcatggcggg gagcacggct acaggcgcag gtgtggccag
    33001 acaaatcgtc gataccagat cattcgccat tccggcagca acacgggcaa tcaaatctgc
    33061 cgtggcaggg gcgagaatca ctaaatcagc ccatttaccc agctcaatat ggcccatagc
    33121 ggcttcggct gccgggtcca gcagactgtc ggaaacggga taaccagaaa ccgcctgcaa
    33181 gctaagtggg gtgataaagg cttttgccgc ttcggtcatg gctacgcgga cgtcggcccc
    33241 gcgatcgcgc aaacgacgca ccagttcagg ggttttatag gcagcaatac cgccgctaac
    33301 gccgagaacg atttttttac cggccaggct catgatgatt tttcctgttg ggtgacacca
    33361 gaagttggcg attttatcac aatacttttg ttgtcgtgcc ttcacctgag aatcactttg
    33421 cgaggcgctt tccaggattg aaaactggcc gtcgatttaa cggaacggct atgacaggat
    33481 gcgagcacca caaaggaggt gaaggtgaaa aacaatgcac agctgttgat gccgcgcgaa
    33541 aaaatgctga agtttggtat tagcgcctta actgatgtcg agctgctggc gctatttctg
    33601 cgtaccggaa cgcgcggtaa agatgtatta accctggcaa aagagatgct ggagaatttc
    33661 ggctctcttt atggcttgtt aacctctgaa tatgaacaat ttagttgtgt tcatggaatt
    33721 ggcgtggcga aatttgccca gttaaagggg attgctgaac tagcgcggcg ttactacaac
    33781 gtgcggatgc gtgaagaaag ccctttactc agcccggaga tgacgcggga atttttacaa
    33841 agccagctca cgggtgagga gcgggagatc tttatggtga tctttctcga ctcacaacac
    33901 cgggttataa cgcatagccg tcttttttcc ggcacgctaa accatgttga agtccatcct
    33961 cgggaaatta tccgcgaagc gataaaaata aatgcctcgg cgctgatcct tgcacataat
    34021 cacccttcgg gttgtgctga acccagtaaa gcggataaac tcattactga acggataata
    34081 aagagttgtc agttcatgga tttacgcgtg ctcgaccata tcgtgattgg gcgtggagag
    34141 tatgtttctt ttgccgaacg cggctggatt taacccgcta tgcgcgatcc ttcgggatct
    34201 ttgtctgttc gggacttgag cacatcgctg agtcagcgta tactacgcca cctttgagaa
    34261 tctcgggttt ggcatttggg cctggcaatc gagagttcac atagaactgc gatgaccggg
    34321 ctgtaaagcc tgacgaggcg ccaatacccc atacgaagct cgagctaatt tgatttttgg
    34381 agaatagaca tgtcccgagt ctgccaagtt actggcaagc gtccggtgac cggtaacaac
    34441 cgttcccacg cactgaacgc gactaaacgc cgtttcctgc cgaacctgca ctctcaccgt
    34501 ttctgggttg agagcgagaa gcgttttgtc accctgcgcg tatctgctaa aggtatgcgt
    34561 gtaatcgata aaaaaggcat cgatacagtt ctggctgaac tgcgtgcccg tggcgaaaag
    34621 tactaagtac ttagaggaaa taaatcatgg ctaaaggtat tcgtgagaaa atcaagctgg
    34681 tttcttctgc tggtactggt cacttctata ccactacgaa gaacaaacgt actaagccgg
    34741 aaaaactgga actgaaaaaa ttcgatccag ttgttcgcca gcacgtgatc tacaaagaag
    34801 cgaaaatcaa ataatttccg ctttgatgta acaaaaaacc tcgctccggc ggggtttttt
    34861 gttatctgct tgcccccata ttgactgcat ctgttcattc ctggagatgc tatgcctgaa
    34921 ttacccgaag ttgaaaccag ccgtcgcggc atagaaccgc atctcgttgg tgcaaccatt
    34981 cttcatgcgg tggtgcgcaa cggacgcttg cgctggccgg tttcagaaga gatctaccgt
    35041 ttaagcgacc aaccagtgct tagcgtgcag cggcgggcta aatatctgct gctggagctg
    35101 cctgagggct ggattatcat tcatttgggg atgtctggca gcctgcgcat ccttccagaa
    35161 gaacttcccc ctgaaaagca tgaccatgtt gatttggtga tgagcaacgg taaagtgctg
    35221 cgctacaccg atccgcgccg ctttggtgcc tggctgtgga ccaaagagct ggaagggcac
    35281 aacgtgctgg cccatcttgg accggagccg cttagcgacg atttcaacgg tgagtacctg
    35341 catcagaagt gcgagaagaa aaaaacggcg attaaaccgt ggctgatgga taacaagctg
    35401 gtggtagggg tagggaatat ctatgccagc gaatctctgt ttgcggcggg gatccatccg
    35461 gatcggctgg cgtcatcact gtcgctggca gagtgtgaat tgctggctcg ggtgattaaa
    35521 gcggtgttgc tgcgttcgat tgagcagggc ggtacaacgc tgaaagattt tctgcaaagc
    35581 gacggtaaac cgggctattt cgctcaagaa ttgcaggttt acgggcgaaa aggcgagccg
    35641 tgtcgtgtgt gcggtacgcc gattgtggcg actaaacatg cgcagcgggc aacgttttat
    35701 tgtcggcagt gccagaagta attgatgcgc gccggatggc ataccatccg gcataaacgc
    35761 tacgctaact tcgccatcag cgcctgatgg acattctccg gcaggaaatg ggtgacatcg
    35821 ccctgatggc gcgccacctc tttcaccaac gatgaagaga taaacgacca ctctttcgac
    35881 ggcatcagaa acacactttc cagttccggc attaagtggc gattcatatg cgccagctgc
    35941 atttcatatt caaaatccgc caccgcacgc aggccacgaa tcagcaccgt agcgtgttga
    36001 ttacgggcga agttcgccat caaatcacta aacccgacca cttccacgtt ccccagatgc
    36061 gcggttgcct gctgtgccag cgccacacgc tcttccagag taaacatcgg ttttttactg
    36121 gggctggcgg caatcgccag aataacgtga tcgaacattt gcgtggcgcg cgtcacgata
    36181 tcgatatgac cattggtaat gggatcgaaa gtacccggat aaatcgcccg tttttgcata
    36241 acaacctcaa tgcgttttcg gtggcaggta aggttccagc agttgaagca ggcgctgtag
    36301 cgcgccctgg ttttgataca gtacttcaac ggcatgacgg ccatagaaac tacggtaatc
    36361 ggcgtcggtg agtaaagagg aaacctcttt tgcaagcgta gtggcatcgg taacggtaat
    36421 cagcccgctt gcctgttcca gccgcgcgca aatgtcttta aagttaaaag tatgcggccc
    36481 catcaatacc ggaatagcgt gtgcggcagc ttccagcgga ttatgcccac cacgttcaac
    36541 cagtgaaccg ccaacaaagg cgagatcggc aatgccgtac agtaacatca gctcgcccat
    36601 cgtatcgcca accacaacct gcgtgctggt ggaagggact tcccctgaag agcgtgtgat
    36661 atagcttagt ccagcctggc ggacaaggtt aatcgcatcc gggaagcgtt ccggatgacg
    36721 gggtaccagg atgagcaata aattagggaa ttgctgtaac aatgcctgat gtgcggcgat
    36781 caccacactc tcttcgcctt cgtgagtgct ggtggcaatc cataccgggc ggtgtggtgc
    36841 ccactggcgg cgcagcgtca cggctttagc agccagttgc ggcgttacag aaatatcaaa
    36901 tttcaggcta ccggtaacgg tcacctgatt attttttgcg cccagcgcca caaaacgcgc
    36961 gccatcttct tcattttgcg cagcaatcag tgtaatacga cgcagcaagc ggcggacgaa
    37021 tttacccagt ttggcgtaac ctgcggccga gcgggcagag agtcgcgcgt tagcgatcac
    37081 cagcggaatt ttacgtttat gtagcgccgc aatcaggtta ggccatagtt cggtttccat
    37141 aatcaacacc agtttagggt cgactttatt caggaaacga ttaagtgcat cgggcaggtc
    37201 atacggcaga taaacgtgct gaacatcctt cccgaaagcc gattgtacgc gctccgaacc
    37261 ggttggcgtc atggttgtaa cggtaatcgg taaatcagga taacgatgac gcagcgcgcg
    37321 caccaacggg attgccgcca gagtttcacc gacggagacg gagtgcagca taatgccgcc
    37381 tggttttagc ggatggcggt aaaaaccgta acgttcaccc cagcgttttc gataggccgg
    37441 agccttacgt ccgcgcaccc agagccgtat ccagatcagc ggctgaataa ggtagagaag
    37501 ggcggtgtaa agcaattcga gcatagtaaa tagctgactt atggatgtgc tggggattct
    37561 atgtatttag ctgtggcttt accattactt ttcccgtttt tgacttaaat agcaatttac
    37621 ccgccaattc ttgctgaaca tctatacttt ttttgtattt ttatgcgatt cattgatttc
    37681 tgcagtacgt gcaggaagga atcgcagagc aacagtgaaa gaatatggtt gctaatgatt
    37741 ttatttgatt gatttcttta ttgaaaatca acaatgtgct taaaaatcat tcgggtcgga
    37801 ttgccgcgaa agaagtgata cactagcgcg tcaatatcag tgcggtatca gtattcaggt
    37861 agctgttgag cctggggcgg tagcgtgctt tttctgcttc accttaatcg gataatctca
    37921 acaaaaagag ttacttgtgg ataagccatt tcgaagaatt ttgctcatta agatgcgttt
    37981 tcatggggat atgttattaa ctactcccgt cattagttcg ctgaaaaaaa attaccctga
    38041 cgcaaaaatc gatgtgctgc tttatcagga caccatcccg atcctgtctg aaaatccaga
    38101 gattaacgcg ctctacggca taaaaaataa aaaagcaaaa gcctcagaaa aaatcgccaa
    38161 cttttttcat ctcatcaagg tattacgtgc caataagtat gaccttatcg tcaatcttac
    38221 cgatcaatgg atggttgcta tactggttcg cttattaaat gcccgtgtga aaatttccca
    38281 ggattatcat catcggcagt ctgctttttg gcgtaaaagt ttcacccatt tggtgccgtt
    38341 gcagggtgga aatgtggtgg aaagtaactt atccgtgctg acaccattgg gacttgattc
    38401 gttggtgaag cagacaacca tgagttaccc gcctgcaagc tggaaacgta tgcgtcgcga
    38461 acttgatcac gctggtgttg gacaaaatta tgtggttatc caacctacgg cgcggcaaat
    38521 cttcaaatgc tgggacaacg ccaagttttc cgctgtgatt gatgccttac atgcttgtgg
    38581 ttatgaagtc gttctgacgt ccggcccaga taaagacgat ctggtctgcg tcaatgaaat
    38641 tgcgcaggga tgccagacgc caccagtaac ggcgctggcc ggaaaggtga ccttcccgga
    38701 acttggtgcg ttaatcgatc atgcgcagct gtttattggc gttgattccg caccggcgca
    38761 tattgccgca gcagttaata cgccgctgat atcgctgttt ggtgcgacag actatatttt
    38821 ctggcgtccc tggtcaaata acatgattca attctgggcg ggagattacc gggaaatgcc
    38881 aacgcgcgat cagcgtgacc gaaatgagat gtatctttcg gttattccgg cggcagatgt
    38941 cattgctgct gtcgataaat tactgccctc ctccacgaca ggtacgtcgt tatgatcgtt
    39001 gctttttgtt tatataaata ttttcccttt ggcggtttgc agcgcgattt tatgcgtatt
    39061 gctcagacag tcgccgcccg aggtcatcat gttcgggttt atacccagtc gtgggaaggc
    39121 gaatgtcctg atgtatttga actgatcaaa gtgccggtta aatcgcatac caatcacggg
    39181 cgcaatgcgg agtattttgc ctgggtgcaa aaacatttac gcgaacatcc cgtcgataaa
    39241 gtcgttggat tcaacaaaat gccggggctg gacgtttatt atgccgctga tgtttgttat
    39301 gccgagaaag tagcgcagga aaaaggcttt ttctatcgcc tgacgtcacg ttatcgccat
    39361 tatgccgcct ttgagcgggc aaccttcgaa cagggcaagc cgacacagct gctgatgctg
    39421 acagataagc aaatcgccga tttccagaaa cattatcaga ctgaagtgga gcgttttcat
    39481 attctgccac cggggattta tcctgatcgt aaatatagcc agcagccagc caatagccgt
    39541 gaaatcttcc gtaagaagaa tggaataacc gaacaacaat atttattgtt gcaggttggt
    39601 tcagacttca cgcgtaaagg tgtcgatcgt tccattgaag cacttgcttc gttaccggat
    39661 tcgctgcgcc acaacacatt gctatatgtt gttgggcagg ataaaccgcg aaaatttgag
    39721 gcgctggcag aaaaacgcgg cgtgcgcagt aatgttcact tcttctcggg gcgcaacgat
    39781 gtctcggaat taatggcggc ggcggattta ttactgcatc ctgcctacca ggaagccgcg
    39841 ggaattgtac tgctggaagc gataactgca ggattaccgg tactaacaac agccgtttgt
    39901 ggctatgcgc attatattgt cgacgccaat tgcggcgagg ctattgctga gccattccgc
    39961 caggaaacat tgaatgagat tttacgcaaa gcgttaacgc aatcttcatt gcgccaggcc
    40021 tgggcggaaa atgcgcgaca ttatgctgat acacaagatt tatacagtct gccagagaaa
    40081 gcggcggaca tcataacggg tggtctggat ggttgaactt aaagagccgt ttgccacgtt
    40141 atggcgcggt aaagatcctt ttgaggaagt taaaaccttg cagggtgagg tatttcgtga
    40201 actggaaact cgccgcactc tgcgctttga aatggcgggc aaaagctatt ttctcaaatg
    40261 gcatcgcggc acgaccctga aagagataat caaaaattta ctctcattgc ggatgccagt
    40321 attaggcgca gaccgcgaat ggaatgcgat tcatcgactg cgggatgtcg gcgttgatac
    40381 tatgtatggg gtggcattcg gcgaaaaagg cattaatccg ctcaccagaa cctcgtttat
    40441 tataaccgaa gatctgacac caaccatcag tctggaagat tactgtgctg actgggcgac
    40501 taacccacca gatgttcgcg taaagcgtat gcttattaag cgtgtcgcga cgatggtgcg
    40561 cgatatgcat gctgcgggca ttaaccaccg tgactgttat atctgtcatt tcctgctgca
    40621 cttgcctttt tccggtaagg aagaggagtt aaaaatttca gtaattgacc tgcaccgggc
    40681 gcagcttcgc acgcgcgttc cacgtcgttg gcgcgataaa gatcttattg gactttattt
    40741 ttcttcgatg aatattggcc tgactcagcg ggatatctgg cggtttatga aagtgtattt
    40801 tgtcgccccg cttaaagaca ttatcaagca ggaacaaggg ctgctgtcgc aagcagaagc
    40861 aaaagccaca aaaatcaggg aaagaacgat tagaaaatcg ttgtaatttc cggagagaaa
    40921 tatgagtgcc cactatttta atccacaaga gatgatcaat aagacaatca tcttcgatga
    40981 aaggccagcg gcgtcagtgg catcatcatt ccatgtttct tatggcattg ataaaaactt
    41041 tctttttggt tgtggtgttt caatcacgtc agttttgtta cataacaacg acgtgagttt
    41101 tgttttccac gtttttattg atgctatccc tgaagccgat atccagcgtt tagcccaatt
    41161 ggcgaaaagc tatcgtacct gtatccagat ccatctagta aattgtgaac ggcttaaggc
    41221 attaccgacg accaaaaatt ggtctattgc catgtatttc cgttttgtaa ttgcagatta
    41281 ctttattgat caacaagata agatccttta cctggatgct gatatcgcct gtcagggaaa
    41341 cttaaagccg ctgataacaa tggatcttgc caataacgtt gctgctgttg ttactgaacg
    41401 cgatgctaac tggtggtcgt tacggggtca aagtctgcag tgtaatgaac ttgaaaaggg
    41461 ttactttaat tcaggtgtcc tgttaattaa tacactagcg tgggcgcagg agtccgtttc
    41521 tgctaaagcg atgtcgatgc ttgctgataa agccatcgtt tcccgtttaa cctatatgga
    41581 tcaagatatc cttaatctta tcctgttagg gaaagttaaa ttcattgatg ctaaatacaa
    41641 tacgcaattt agtttaaatt atgaattaaa aaaatcattt gtttgtccaa ttaatgatga
    41701 aaccgtatta attcattatg tcggcccgac aaaaccctgg cattactggg ccggttatcc
    41761 aagtgcgcaa ccttttatca aagccaaaga agcatcgccc tggaaaaatg aaccgttaat
    41821 gcggccagtt aactcaaact atgctcgtta ttgcgccaag cataatttta aacaa
//
LOCUS       DAPOZO010000043        41750 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPOZO010000043 DAPOZO010000000
VERSION     DAPOZO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.043"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            59..1138
                     /gene="fbaA"
                     /locus_tag="ROI21_002348"
     CDS             59..1138
                     /gene="fbaA"
                     /locus_tag="ROI21_002348"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDX5111261.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            1496..2356
                     /gene="mscS"
                     /locus_tag="ROI21_002349"
     CDS             1496..2356
                     /gene="mscS"
                     /locus_tag="ROI21_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDX5111262.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            2495..3130
                     /gene="argO"
                     /locus_tag="ROI21_002350"
     CDS             2495..3130
                     /gene="argO"
                     /locus_tag="ROI21_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDX5111263.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VMKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLALLAAWLAPRLRTAKAQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            3223..3963
                     /locus_tag="ROI21_002351"
     CDS             3223..3963
                     /locus_tag="ROI21_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDX5111264.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            4130..5026
                     /locus_tag="ROI21_002352"
     CDS             4130..5026
                     /locus_tag="ROI21_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5111265.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(5023..6501)
                     /gene="scpC"
                     /locus_tag="ROI21_002353"
     CDS             complement(5023..6501)
                     /gene="scpC"
                     /locus_tag="ROI21_002353"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDX5111266.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLLLQSGVGNINNAVMARLGENP
                     VIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(6525..7310)
                     /gene="scpB"
                     /locus_tag="ROI21_002354"
     CDS             complement(6525..7310)
                     /gene="scpB"
                     /locus_tag="ROI21_002354"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDX5111267.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(7321..8316)
                     /gene="meaB"
                     /locus_tag="ROI21_002355"
     CDS             complement(7321..8316)
                     /gene="meaB"
                     /locus_tag="ROI21_002355"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDX5111268.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(8309..10453)
                     /gene="scpA"
                     /locus_tag="ROI21_002356"
     CDS             complement(8309..10453)
                     /gene="scpA"
                     /locus_tag="ROI21_002356"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDX5111269.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVHQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(10657..11550)
                     /gene="argP"
                     /locus_tag="ROI21_002357"
     CDS             complement(10657..11550)
                     /gene="argP"
                     /locus_tag="ROI21_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDX5111270.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            11692..11922
                     /locus_tag="ROI21_002358"
     CDS             11692..11922
                     /locus_tag="ROI21_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111271.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLVNGELVRLFAPWSGIP
                     RLLHALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            11978..12637
                     /gene="rpiA"
                     /locus_tag="ROI21_002359"
     CDS             11978..12637
                     /gene="rpiA"
                     /locus_tag="ROI21_002359"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDX5111272.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            12893..14125
                     /gene="serA"
                     /locus_tag="ROI21_002360"
     CDS             12893..14125
                     /gene="serA"
                     /locus_tag="ROI21_002360"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDX5111273.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            14354..14413
                     /gene="ibsC"
                     /locus_tag="ROI21_002361"
     CDS             14354..14413
                     /gene="ibsC"
                     /locus_tag="ROI21_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDX5111274.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(14514..15116)
                     /locus_tag="ROI21_002362"
     CDS             complement(14514..15116)
                     /locus_tag="ROI21_002362"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDX5111275.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(15137..15320)
                     /gene="ssrS"
                     /locus_tag="ROI21_002363"
     ncRNA           complement(15137..15320)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ROI21_002363"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(15362..15691)
                     /gene="zapA"
                     /locus_tag="ROI21_002364"
     CDS             complement(15362..15691)
                     /gene="zapA"
                     /locus_tag="ROI21_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDX5111276.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            15859..16437
                     /gene="ygfB"
                     /locus_tag="ROI21_002365"
     CDS             15859..16437
                     /gene="ygfB"
                     /locus_tag="ROI21_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDX5111277.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            16463..17788
                     /gene="pepP"
                     /locus_tag="ROI21_002366"
     CDS             16463..17788
                     /gene="pepP"
                     /locus_tag="ROI21_002366"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDX5111278.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            17785..18963
                     /gene="ubiH"
                     /locus_tag="ROI21_002367"
                     /gene_synonym="visB"
     CDS             17785..18963
                     /gene="ubiH"
                     /locus_tag="ROI21_002367"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDX5111279.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITSVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLECGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHLLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDIGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            18986..20188
                     /gene="ubiI"
                     /locus_tag="ROI21_002368"
                     /gene_synonym="visC"
     CDS             18986..20188
                     /gene="ubiI"
                     /locus_tag="ROI21_002368"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDX5111280.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDMFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(20262..20345)
                     /locus_tag="ROI21_002369"
     CDS             complement(20262..20345)
                     /locus_tag="ROI21_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111281.1"
                     /translation="MSKNTKSKIRGIRKYNAKTEVKLVYFK"
     gene            20636..21730
                     /gene="gcvT"
                     /locus_tag="ROI21_002370"
     CDS             20636..21730
                     /gene="gcvT"
                     /locus_tag="ROI21_002370"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDX5111282.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRHAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            21754..22143
                     /gene="gcvH"
                     /locus_tag="ROI21_002371"
     CDS             21754..22143
                     /gene="gcvH"
                     /locus_tag="ROI21_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDX5111283.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            22262..25135
                     /gene="gcvP"
                     /locus_tag="ROI21_002372"
     CDS             22262..25135
                     /gene="gcvP"
                     /locus_tag="ROI21_002372"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDX5111284.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTTLISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGAWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREIAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(25299..26732)
                     /gene="bglA"
                     /locus_tag="ROI21_002373"
     CDS             complement(25299..26732)
                     /gene="bglA"
                     /locus_tag="ROI21_002373"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDX5111285.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKYKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKMEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFDWYKEVIASNGEKL"
     gene            26777..27088
                     /locus_tag="ROI21_002374"
     CDS             26777..27088
                     /locus_tag="ROI21_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDX5111286.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLLELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            27252..27911
                     /locus_tag="ROI21_002375"
     CDS             27252..27911
                     /locus_tag="ROI21_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDX5111287.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     IKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(27988..28968)
                     /gene="ygfZ"
                     /locus_tag="ROI21_002376"
     CDS             complement(27988..28968)
                     /gene="ygfZ"
                     /locus_tag="ROI21_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDX5111288.1"
                     /translation="MAFTPFPPRQPTASARLPLTLITLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            29211..29477
                     /gene="sdhE"
                     /locus_tag="ROI21_002377"
     CDS             29211..29477
                     /gene="sdhE"
                     /locus_tag="ROI21_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDX5111289.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            29458..29865
                     /locus_tag="ROI21_002378"
     CDS             29458..29865
                     /locus_tag="ROI21_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDX5111290.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(29905..30426)
                     /gene="fldB"
                     /locus_tag="ROI21_002379"
     CDS             complement(29905..30426)
                     /gene="fldB"
                     /locus_tag="ROI21_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDX5111291.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     ILHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQNW
                     CEQILNEMAEHYA"
     gene            30538..31434
                     /gene="xerD"
                     /locus_tag="ROI21_002380"
     CDS             30538..31434
                     /gene="xerD"
                     /locus_tag="ROI21_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDX5111292.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            31459..32169
                     /gene="dsbC"
                     /locus_tag="ROI21_002381"
     CDS             31459..32169
                     /gene="dsbC"
                     /locus_tag="ROI21_002381"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDX5111293.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            32175..33908
                     /gene="recJ"
                     /locus_tag="ROI21_002382"
     CDS             32175..33908
                     /gene="recJ"
                     /locus_tag="ROI21_002382"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDX5111294.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELKRS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            33999..35097
                     /gene="prfB"
                     /locus_tag="ROI21_002383"
     CDS             join(33999..34073,34075..35097)
                     /gene="prfB"
                     /locus_tag="ROI21_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDX5111295.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNETVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            35107..36624
                     /gene="lysS"
                     /locus_tag="ROI21_002384"
     CDS             35107..36624
                     /gene="lysS"
                     /locus_tag="ROI21_002384"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDX5111296.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(36667..37215)
                     /gene="idi"
                     /locus_tag="ROI21_002385"
     CDS             complement(36667..37215)
                     /gene="idi"
                     /locus_tag="ROI21_002385"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDX5111297.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQQGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            37270..37341
                     /locus_tag="ROI21_002386"
     CDS             37270..37341
                     /locus_tag="ROI21_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111298.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(37338..37463)
                     /locus_tag="ROI21_002387"
     CDS             complement(37338..37463)
                     /locus_tag="ROI21_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111299.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(37465..38913)
                     /gene="uacT"
                     /locus_tag="ROI21_002388"
     CDS             complement(37465..38913)
                     /gene="uacT"
                     /locus_tag="ROI21_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDX5111300.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            39349..41268
                     /gene="ygfT"
                     /locus_tag="ROI21_002389"
     CDS             39349..41268
                     /gene="ygfT"
                     /locus_tag="ROI21_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDX5111301.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAMAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQPDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAW"
     gene            41268..>41750
                     /locus_tag="ROI21_002390"
     CDS             41268..>41750
                     /locus_tag="ROI21_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDX5111302.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDCISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     P"
BASE COUNT         9991 a        10473 c        11180 g        10106 t
ORIGIN      
        1 cagcacatgc aacttgaagt atgacgagta taaggcccga cgatacagga caagagacat
       61 gtctaagatt tttgatttcg taaaacctgg cgtaatcact ggtgatgacg tacagaaagt
      121 tttccaggta gcaaaagaaa acaacttcgc actgccagca gtaaactgcg tcggtactga
      181 ctccatcaac gccgtactgg aaaccgctgc taaagttaaa gcgccggtta tcgttcagtt
      241 ctccaacggt ggtgcttcct ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg
      301 tgctgctatc ctgggcgcga tctctggtgc gcatcacgtt caccagatgg ctgaacatta
      361 tggtgttccg gttatcctgc acactgacca ctgcgcgaag aaactgctgc cgtggatcga
      421 cggtctgttg gacgcgggtg aaaaacactt cgcagctacc ggtaagccgc tgttctcttc
      481 tcacatgatc gacctgtctg aagaatctct gcaagagaac atcgaaatct gctctaaata
      541 cctggagcgc atgtccaaaa tcggcatgac tctggaaatc gaactgggtt gcaccggtgg
      601 tgaagaagac ggcgtggaca acagccacat ggacgcttct gcactgtaca cccagccgga
      661 agacgttgat tacgcataca ccgaactgag caaaatcagc ccgcgtttca ccatcgcagc
      721 gtccttcggt aacgtacacg gtgtttacaa gccgggtaac gtggttctga ctccgaccat
      781 cctgcgtgat tctcaggaat atgtttccaa gaaacacaac ctgccgcaca acagcctgaa
      841 cttcgtattc cacggtggtt ccggttctac tgctcaggaa atcaaagact ccgtaagcta
      901 cggcgtagta aaaatgaaca tcgataccga tacccaatgg gcaacctggg aaggcgttct
      961 gaactactac aaagcgaacg aagcttatct gcagggtcag ctgggtaacc cgaaaggcga
     1021 agatcagccg aacaagaaat actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc
     1081 gatgatcgct cgtctggaga aagcattcca ggaactgaac gcgatcgacg ttctgtaaga
     1141 tattcctttc tgcttatctc aaggcccgct ctgcgggtct ttttttcgcc aaaagcaaaa
     1201 ttgcctgatg cgctacgctt atcaggccta cacgtatcct gcaatatatt gaatttgcag
     1261 gtttttgtag gccggataag gcgttcacgc cgcatccggc atgacaaagc gcacattgat
     1321 atctgtctga gccccgctct gcggggattt tttcgccaaa agcaaggtga ttcagatgag
     1381 aaatctgtga tctatttggc aaaattatgc tttattgttt acccttgtca gactgcccgt
     1441 cataaggcgg cggagtgtat ttctccattt tgagtcagtt gaaaaggaat attgaatgga
     1501 agatttgaat gttgtcgata gcataaacgg cgcgggaagc tggctggtag ctaaccaggc
     1561 gctgctgcta agttatgcag taaacatcgt ggcggcactc gcgatcatca tcgttggttt
     1621 gattatcgcg cggatgattt ccaacgcggt gaatcgcctg atgatctccc gtaaaatcga
     1681 tgccactgtt gctgattttc tttctgcatt agtccgttac ggtattatcg cctttacgct
     1741 aatcgctgca ctgggacgcg tgggtgtaca aaccgcgtca gtcattgctg tactcggtgc
     1801 cgcaggctta gctgttggtc tggctttgca ggggtcactg tctaacctgg ccgctggcgt
     1861 gttacttgtc atgttccgcc cgttccgtgc cggagaatat gttgacctgg gcggcgtagc
     1921 cggtactgtg ctgagtgtgc agattttctc caccaccatg cgtactgcag acggtaaaat
     1981 tatcgttatt ccgaacggta aaattattgc cggaaatatt attaacttct cccgcgagcc
     2041 agttcgccgt aacgaattta ttattggcgt ggcgtatgat tccgatatcg atcaggttaa
     2101 gcagatcctg accaatatta tccagtctga agatcgcatt ttgaaagatc gcgaaatgac
     2161 tgtgcgcctg aacgaacttg gtgcatcgtc gattaatttc gtggtccgcg tctggagcaa
     2221 cagcggcgat ctgcaaaacg tgtactggga tgtgctggag cgtattaaac gtgaatttga
     2281 tgccgccggt atcagcttcc cgtacccgca aatggatgtg aactttaagc gggtgaaaga
     2341 agacaaagct gcgtaatcaa cgctgaggcc agataatact ctctggcctc tctcttatta
     2401 gtttttctga ttgccaatta atattatcaa tttccgctaa taacaatccc gcgatatagt
     2461 ctctgcatca gatacttaat tcggaatatc caacgtgttt tcttattact ttcaaggtct
     2521 tgcacttggg gcggctatga tcctaccgct cggtccacaa aatgcttttg tgatgaatca
     2581 gggcatacgt cgtcagtacc acattatgat tgccttactt tgtgctatca gcgatttggt
     2641 cctgatttgc gccgggattt ttggtggcag cgcgttattg atgcagtcgc cgtggttgct
     2701 ggcgctggtc acctggggcg gcgtagcctt cttgctgtgg tatggttttg gcgcttttaa
     2761 aacagcaatg agcagtaata ttgagttagc cagcgccgaa gtcatgaagc aaggcagatg
     2821 gaaaattatc gccaccatgt tggcagtgac ctggctgaat ccgcatgttt acctggatac
     2881 ttttgttgta ctgggcagcc ttggcgggca acttgatgtg gaaccaaaac gctggtttgc
     2941 actcgggaca attagcgcct ctttcctgtg gttctttggt ctggctcttc tcgcagcctg
     3001 gctggcaccg cgtctgcgca cggcaaaagc acagcgcatt atcaatctgg ttgtgggatg
     3061 tgttatgtgg tttattgcct tgcagctggc gagagacggt attgctcatg cacaagcctt
     3121 gttcagttag gcgctatctg atggaaaaat aaaacagagg cgctaagctt gcctccagag
     3181 gtcctgaatt ttgggcaagt caattaacac ggagagacta acgtgaagtt caaagttatc
     3241 gccctggcgg cattaatggg tattagcggg atggcagcgc aggctaacga attgccggat
     3301 ggaccgcata ttgtcacctc cggtacggca agcgtggatg cggtgccaga cattgccact
     3361 cttgcgattg aagttaacgt ggccgcgaag gatgccgcta ctgccaagaa acaggcagat
     3421 gagcgcgtcg cacaatacat ttccttcctt gaactcaatc agatcgcgaa aaaagatatc
     3481 agctcagcga acttacgcac ccagccagat tatgattatc aggatggtaa aagtatcctt
     3541 aaaggctacc gcgctgtgag aacggtggaa gtcacgctcc gtcagttaga caaactgaat
     3601 tccttgctgg atggcgcgct gaaggcgggt cttaacgaaa ttcgttctgt gtcgctgggc
     3661 gtggcgcagc cggatgccta taaagacaaa gcgcgtaagg cagcgattga taacgcgatt
     3721 catcaggcgc aggaactggc gaacggcttt catcgtaaac tggggccggt atatagcgtg
     3781 cgctaccatg tttccaacta tcagcccagc ccaatggtgc ggatgatgaa agccgatgcc
     3841 gcgccggtgt ccgcccagga aacttacgag caggccgcta ttcagtttga tgatcaggtc
     3901 gatgtggtct tccagttaga acctgtggat caacaacccg ctaaaacacc tgcagcacaa
     3961 taatatttgt agcccgacgt attcgtcggg ctaatgcctt tacccgatat tgcctccttt
     4021 ttctgtaatt ttctgtaaaa ccatgacgta atcacctatc tttgctcgcg gtttcatcaa
     4081 ggataggctg actgaaacgg ttaagcggac aaggatgcgg gggaggggga tggacatttt
     4141 tatttcgaaa aagatgcgca atttcatttt attagcgcaa accaataata ttgcccgggc
     4201 ggcagaaaaa atccatatga cggcttcgcc atttggtaaa agcattgccg cgctggaaga
     4261 acaaattggc tatacgctat ttacccgcaa agataataat atcagcctca ataaagccgg
     4321 acaggaactg tatcaaaaac tgttcccggt ttatcaacgg ctttctgcga ttgataatga
     4381 aatccataac tcagggcgtc gttcacgaga aattgtgatt ggcatagata atacttatcc
     4441 gacaattatt ttcgatcagt tgattagtct cggagataag tatgaaggcg tgaccgccca
     4501 gccagttgag ttcagcgaaa atggcgttat cgataatctt tttgaccgcc agttagattt
     4561 tattatctcc ccacagcatg tatctgcacg agttcaggaa ctggaaaacc tgactatcag
     4621 tgaactacca ccgctgcgat taggctttct cgtttctcgc cgttatgaag agcgacaaga
     4681 gcaagagctg cttcaggagc tgccctggct acaaatgcgc tttcagaacc gggccaactt
     4741 cgaagcaatg attgatgcga atatgcgacc ttgtgggatc aatccaacca ttatttatcg
     4801 tccctacagt tttatggcca aaatcagcgc cgtggagcgc ggacattttc tgacggtgat
     4861 cccgcatttt gcctggcgac tggtgaatcc ggcaacgctg aaatattttg atgcaccgca
     4921 caggccgatg tatatgcagg aatatcttta ttcaatcaga aatcatcgtt ataccgccac
     4981 gatgcttcag catattgctg aagatcgtga cgggacgagt cattaaccca gcatcgagcc
     5041 ggttgcaatt aaattacggt gtaagtcgaa gacgtggcta agatcgtggt gaatatgtcc
     5101 gccaggcgca ttttccagat agcgatgcag ataatcccga tacataggat gtgcacaatt
     5161 atcaatgata gtgcgggcgc gttgaagcgg ggaaagaccg cgcagatcgg cgatcccttg
     5221 ttcggtgatg atcactttga cgctgtgttc gctgtgatca acatggctgc acattggcac
     5281 gacggttgag atcttgcctt ctttagcaat cgacggggcc ataaagatcg acagatacgc
     5341 gttgcgttca aaatcaccgc tgccgccgat gccgttcatc agatcgaccc cggctacgtg
     5401 tgttgagttg gcatgcccgt aaatatcaaa ctccaggccg acgttcagag cgatgacgcc
     5461 cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca caatgcggct
     5521 ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga ttgtcaggct
     5581 ggaggcgctg gccccgctga ttttgccggt ttccagtaaa tgcaccaccg attcctgtag
     5641 cacttccgaa tacatcataa acggaggaat taccgggttt tcccccagac gcgccattac
     5701 cgcattattg atattgccca cgccactttg cagcagcaga aattccggcg gaatacgccc
     5761 atgcgccatt tcctgcaata agaacgtgac cacgttatcg gcaatctgct ggcacatggg
     5821 attttgctta tccagcatat taccggcgtc gggcaagttg gtttccacga cggcgacaat
     5881 ctttttcgga tcgatttgca catagcgggt accgacgcga tccattgcat ggaagatcga
     5941 cacgctattg cgccgcggtg gcgcgccagg aatcacaata tccgccagtt ctgcaacgcg
     6001 cggatcgtga tagtgattga gttcaatgat cactttcttc gcccgcagca gccaggtcgg
     6061 cgcattaccg atcccgctgg ttaaccagac tcgaccatcc ggtgccagtg ccgatgcttc
     6121 aatgacggca acatcaatgt cgccgaagaa accgtaattg accatttgcg ccacttcgct
     6181 caaatgcagg tcaacgaaac tcaccgcgcc ctgattgatc tttttacgta aaccggacga
     6241 tgtttgatat ggcgcacgcc aggaaacagc atcggcgtca gaaagtacat cgtcagcggc
     6301 ggcgctgatt gacgcacccg tcagaaggcg aatttgatac ggctttttgg cctcatgctg
     6361 ttcgttagct ctgcgggcaa tcgcggtggg tagggctttc ggcgaacccg ccggggtaaa
     6421 gccgctaaat gccaccatgt cgttatgctg gataatttct gccgcttcat tggcggtcat
     6481 ccttgtccac tgagtttcca tttttactcc ttcgttcgca gggattaatg accaacgaaa
     6541 ttaggtttac gtttttcgag gaaagcgttc atcccttcct ggtaatcttc gctgtcatac
     6601 accgcgcggc gcatcccctg aatacgttca aattcatcgg agttcatggt gtgtgcttcg
     6661 cccagtacac gcagctcttc tttgataacg gcaatggcta acggcgcttt ctcagagatg
     6721 tggtgcgcca tttgtaaggt gaaatcttcc agttcttcca cttccacaac atggttgagg
     6781 atgccgacag ccagcgcgcg ctgggcggtg attggcgaag cggtaaaaat cagctctttg
     6841 acaatgtgga agcccgcgtc gcgggtcagg ttgtgaatgc cgaccaggtt atacgggacg
     6901 ccgaggttta caggcgtcat tgagaaggtt gaggtactgg cggcgatgat cagatcggaa
     6961 ctcatgatca tttcaaatgc gccaccccaa acactacctt ccaccatcga aatgatcggt
     7021 ttcgggaatt tttggatcat gcgggtgatt tgacgcaatg gatcatcata ggagagcgga
     7081 tcgcgaccgc cagacggcag ttcgtgaata tcgtgacctg cggagaagac tttggatcca
     7141 ctcggtgcgc gcaaaatgat acagcgaatt tccggccggt tgagatcgct taacgcctgc
     7201 ataagatcat caataaagac tttacttaag gcattaagtt ttcggccata gttaaactca
     7261 atgaccgcca ctttgttgat agtgacaacg ttaacatact gataagacat aaaaattcct
     7321 ttaatcaaaa tattgcgtct ggataaattc actgagctgc cgcaggccgg tgcgcggtga
     7381 gagcgtattg tttttgaccg ctaaaagcgt ctggcggtaa tagcgatcga aatcttcatt
     7441 cgcgaacagg tgattcagta cttcttcttc ggtctgctta cgcagccatt ccaccgattg
     7501 ttgttgccgc acttgttgta aacgaccact ggcagttagc gcggttttga agtcgatgat
     7561 ggcgtgccag atctcatcga ttccacgttt ttccagtgcg ctacaagtca gaacccgtgg
     7621 ctgccattcg tcgtatttac gtcgcagaat atgcagggca ctctcgtaca tatgccgggc
     7681 aatggcgaca ttggtatggt tatcgccatc gtctttgttg ataacgatca gatcagccac
     7741 ttccatcagc ccttttttaa tgccctgcag atcatcgccg ccaccggcaa tttgcaacga
     7801 gataaaacag tccaccatgc gggcgacttc tgtttccgac tgcccgacgc caaccgtttc
     7861 gacaatcact acgtcataac ccgctgcttc gcataacagc attaattccc gcgctcgctg
     7921 actggcaccg cccagatgac cggaggatgg taccgggcga ataaacgccg cttcggcacg
     7981 cgccaggtca ttcatgcggg ttttatcccc gagaatgcta ccgccagtga ccgggctgct
     8041 gggatcgacc gcaataaccg cgacctttaa tccctctcga atcaacaaca tgccaaaggc
     8101 ctcaagaaag gtacttttcc ccgcgccggg ggtgccggta acgcccagtc gcagggtgtt
     8161 accgcagtac ggcataatgg catcaagcag ctgcgtactt agtgcctgat gacgcgggtg
     8221 acggctttcc accagcgtca tggcctgggc gagtgtggca cgctcaccct gacgtaagcg
     8281 gcgaatactt tctgccagcg tggcttcatt aatcatgatg ctggcttatc agattcagta
     8341 cgtcgcgcac actgtcgagc ataggtgtac ctggaccata aatcgccgcc acgccgcgct
     8401 cttgcaggaa ggcgtaatcc tgcggcggaa tgacgccacc cgcgaccacg cagatatctt
     8461 cgcgtcccca ttttttcagc gcttcgacca gttccgggat cagcgtttta tgaccggcag
     8521 ccagtgagga tgcgcccact acgtgaacgt cgttttctac ggccaggcgg gcgatctctt
     8581 caggtgtaga gaacatcggg cttaaatcta cgtcgaaacc gagatcggaa taggcgctgg
     8641 cgatcacttt cgcgccgcga tcgtgtccat cctggcccat cttagcgatc agaatgcgcg
     8701 ggcgacgacc attgtcggca aggaactgct ccgtttgcgc aacaatggca tcgaactcgg
     8761 aggccgattt ctcagactga tgatagcttt gcgcaatcac gccggtaaca cactggcttg
     8821 gcaccagata acggtcgaaa gcgacttcca gcgcatcgga aatttcaccc agggtggcgc
     8881 gaacgcgagc ggcattaaca gcggcagcca gcaggttttc gttatgctgt gcggcgtgag
     8941 tcagggcgtt caacgcggcg gttacggcgg catcatcacg ggtggcgcga atgcgttcca
     9001 gcgaagcaat ttgctcgtta cgcaccatca cgttgtcgat ctcaagtaca tcggtttcgt
     9061 cttcgtgatc cagtttgtac ttgttgacac caacgatgac acgcttgccc tggtcgatca
     9121 gcgactgttc gcgcgctgag gcctcttcga tcattcgttt tggcagacct gcttcgatcg
     9181 ctttcgccat gccaccggct tcgtcgatct gttggataat agctctggct tgtttgacga
     9241 tttgatcggt cagcgactca atgtaatagg atccggccag tggatcgacg gtgcggcaga
     9301 gttctgattc ttcctggatg atgatctggg tgttgcgggc aatgcgtgct gagaaatcgg
     9361 taggcaaacc aagcgcttcg tcaaaggcgt tggtatgcag tgactgggta ccgcccagcg
     9421 tcgcagccag cgcttcaatg gtggtgcgga taacgttgtt atacggatcc tgttcagtca
     9481 ggctccagcc tgaggtctgg cagtgggtac gcagcgccag tgatttcggg tcctgtgcgc
     9541 caaatccact gaccgcttcg ctccataaat aacgtgccgc acgcaacatg gcgacgttca
     9601 taaacagatc catgccgatg ccgaagaaga acgacaggcg aggagcgaag tcatcaattt
     9661 tcagtccggc agagattgct gctttgatgt actcaatccc atcagcgagc gtaaatgcta
     9721 cctgctgcac gcagttggca cccgcttcac ccatgtggta accgctgata ctgatggtat
     9781 taaatcgcgg catgttgccg gaacaccagg cgatgatgtc ggcgataatg cgcattgacg
     9841 gttttggtgg gtaaatatag gtgttgcggc agaggtactc tttgagaata tcgttttgaa
     9901 tggtgccggt cagtttatca ggtgtaacac cttgctcttc tgcggcgacg atataaaacg
     9961 ccagtactgg tagcactgcg ccattcatgg tcatcgaaac cgacatttta tccagcggga
    10021 tctggtcgaa caggactttc atatcttcca cggtgtcgat agcgacgccc gctttgccga
    10081 cgtcgcccgc cacgcgcggg ttatcggagt cgtagccacg gtgggtggca aggtcaaacg
    10141 caacggaaag acctttttgc ccggcggcca ggttacggcg ataaaaagcg ttggactctt
    10201 ttgctgttga aaaaccagca tactgacgga tggtccacgg ttgggcggta tacatagtgg
    10261 cacgcgggcc acgaacgtag ggcggcaaac caggaagggt acctgtcacc tccagattat
    10321 cgagatcggc ttcggtatac agcggcttga tggcgatccc ttccgcggtt tgatgaacca
    10381 gcgagtcgac agttttctcc cgacggctca attccttgtt ggcaagctgt tgccactcct
    10441 gcacgttaga cattatttgc tccgtaaaac gcaatcaaaa tgagggtatt tgcgcctacg
    10501 gtgagctttt tacgtgggtt tgtcgccagc aaaaaaaatg cattcagacg tcgttttttg
    10561 ccgcctgaga tttgtctatt ttgtgatgtg attcaaggaa tacaaaaaga agtgaggaca
    10621 acgcctgata tgtgctatca ggcattattt gatggattaa tcctgacgaa ggactttgtg
    10681 accataatcg agtaacgcat cagtgacttt acgcatcatg cggctttcag gagcaaagcg
    10741 gtgccagtag agcatccgtc gttgaaatag cccaggcgtt aagtcaatca gttcaccgct
    10801 ggccagctct ttctcgattt gcaggtgcgg gatcatacag caggtggtgc cctggcgagc
    10861 aagttgtacg aacgcttctg aagaattaac gatatggcag ggcacgctgc ctggaggcag
    10921 atcgaagttt tgctgcaaaa aggcctggtg catatcgtca agatggtcaa acgcgaccac
    10981 tggcgctttc agtaatgccg aacgcgttac gccgttaggg aaatattttt cggcaaaggg
    11041 ttttgagctg acgaacagat agtcgagcgc accaagttta tcgacaagac aactcggcag
    11101 cgcctgatgt tgaatactca ccgcgccgac cacttcgccg cggcgcagac gttcctgagt
    11161 gcgggtttca tcttctacct gcaagttgag gcggataggc gaatcagcca acacaggagc
    11221 cagtgcagga agcaaccacg tcgccagact gtcggcgttg accgccagtg aaagcagcag
    11281 cggagtcgaa ccggtttgtt catcgcccag ccactcttct tccagcaact ccacctggcg
    11341 cagcagtgcc agcagttttt gcccttgttc cgtcgggcgc ggcggtacgg tacgcaccaa
    11401 cagcggctgc ccgaacatat tttccagttg cttaatgcgc tgtgagacgg ctgattgtgt
    11461 aatgcacagc ttttgtgccg cgcgctcaaa tcctcgttca cgtatcaccg catccagtgc
    11521 ctgtaatgtt ctgtagtccg ggcgtttcat tgctgttgtc tgctcctggt aaatgctttt
    11581 tcctgcacta tgacacaatt ttgtgtcagg ttgcatatac cgaagccgtc ggcgggtaac
    11641 atttgtattc gtggttaaac gcgcagctta aaagatcagg gatttgcgct aatgcatttc
    11701 gctcagcgag ttcgcgccct ggttgtgttg aatggcgtgg cgttattgcc tcaatttgcc
    11761 tgtaaacagg ggcttgtgaa cggtgaactg gtgcgcctgt ttgctccgtg gagcggcata
    11821 cccagattgt tgcatgcctt atttgcgggg cgaaagggga tgcctgccat tgcgcgatat
    11881 tttatggatg agttaaccac gcggcttgcc aacggggtct gaatcgcttt ttttgtatat
    11941 aatgcgtgtg aaatttcata ccacaggcga aacgatcatg acgcaggatg aattgaaaaa
    12001 agcagtagga tgggcggcac ttcagtatgt ccagcccggc accattgttg gtgtaggtac
    12061 aggttccacc gccgcacact ttattgacgc gctcggtaca atgaaaggcc agattgaagg
    12121 ggccgtttcc agttcagatg cttccactga aaaactgaaa agcctcggca ttcacgtttt
    12181 tgatctcaac gaagtcgaca gccttggcat ctacgttgat ggcgcagatg aaatcaacgg
    12241 ccacatgcaa atgatcaaag gcggcggcgc ggcgctgacc cgtgaaaaaa tcattgcttc
    12301 ggttgcagaa aaatttatct gtattgcaga cgcttccaag caggttgata ttctgggtaa
    12361 attcccgctg ccagtagaag ttatcccgat ggcacgtagt gcagtggcgc gtcagctggt
    12421 gaaactgggc ggtcgtccgg aataccgtca gggcgtggtg accgataatg gcaacgtgat
    12481 cctcgacgtc cacggcatgg aaatccttga cccgatagcg atggaaaacg ccataaatgc
    12541 gattcctggc gtggtgactg ttggcttgtt tgctaaccgt ggcgcggacg ttgcgctgat
    12601 tggcacacct gacggtgtca aaaccattgt gaaatgatct gacgggggaa cctcccccgt
    12661 taaaaaaatt ctcttcatta aatttggtga catgtgtcac gtttttacct ggctattgtc
    12721 gattgctcta aataaatctt ctaaaccagc atattcatcc aagaattacc tttgcgtgat
    12781 atttcctcaa catcgcgacg caaacgttca tattgccgca atattatttt ttgatatgtt
    12841 gaaaggcgga cgcaaatccg cacacaacat ttcaaaagac aggattgggt aaatggcaaa
    12901 ggtatcgctg gagaaagaca agattaagtt tctgctggta gaaggcgtgc accaaaaggc
    12961 gctggaaagc cttcgtgcag ctggttacac caacatcgaa tttcacaaag gcgcgctgga
    13021 tgatgaacaa ttaaaagaat ccatccgcga tgcccacttc atcggcctgc gatcccgtac
    13081 ccatctgact gaagacgtga tcaacgccgc agaaaaactg gtcgctattg gctgtttctg
    13141 tatcggaaca aaccaggttg atctggatgc ggcggcaaag cgcgggatcc cggtatttaa
    13201 cgcaccgttc tcaaatacgc gctctgttgc ggagctggtg attggcgaac tgctgctgct
    13261 attgcgcggc gtgccggaag ccaatgctaa agcgcaccgt ggcgtgtgga acaaactggc
    13321 ggcgggttct tttgaagcgc gcggcaaaaa gctgggtatc atcggctacg gtcatattgg
    13381 tacgcaattg ggcattctgg ctgaatcgct gggaatgtat gtttactttt atgatattga
    13441 aaacaaactg ccgctgggca acgccactca ggtacagcat ctttctgacc tgctgaatat
    13501 gagcgatgtg gtgagtctgc atgtaccaga gaatccgtcc accaaaaata tgatgggtgc
    13561 gaaagagatt tcactaatga agcccggctc gctgctgatt aatgcttcgc gcggtactgt
    13621 ggtggatatt ccggcgctgt gtgacgcgct ggcgagcaaa catctggcgg gggcggcaat
    13681 cgacgtattt ccgacggaac cggcaaccaa tagcgatcca tttacctctc cgctgtgtga
    13741 attcgacaac gtccttctga cgccacacat tggcggttcg actcaggaag cgcaggagaa
    13801 tatcggcctg gaagttgcgg gtaaattgat caagtattct gacaatggct caacgctctc
    13861 tgcggtgaac ttcccggaag tctcgctgcc actgcacggt gggcgtcgtc tgatgcacat
    13921 ccacgaaaac cgtccgggcg tgctaactgc gctgaacaaa atcttcgccg agcagggcgt
    13981 caacatcgcc gcgcaatatc tacaaacttc cgcccagatg ggttatgtgg ttattgatat
    14041 tgaagccgac gaagacgttg ccgaaaaagc gctgcaggca atgaaagcta ttccgggtac
    14101 cattcgcgcc cgtctgctgt actaattccc cttctctgaa aattaacggg caggtcactg
    14161 acttgcccgt ttttttatcc cttttccaca ccgttgccgc attgcgcatt ctactttgcg
    14221 agtctgctcc agaaaataaa tcaaagtcag atcaatgcgt tgtgtttatt taatggggat
    14281 ttacgatggc aggacagcac agggctgtaa cggtaaagcc ctcaccgaag cgagggcttg
    14341 aaggagaagg gttatgatgc gacttgtcat catactgatt gtactgttac tcataagttt
    14401 cccagcttat taacagtcag tctcagggga ggagtaatcc tcccttaccc ttactcacta
    14461 aattaggtta aagaatcaac gatgtcaatc agggcgatgc aggtgtgtcg ccctcaccac
    14521 tcccagactt tcgacggtgt aaccaccgca ggaagaggga tatcccactc ttcaacgggg
    14581 agtttttcca ccaactgaca atcatgcgca taacccaccg gttgcatttt atagtgctgc
    14641 cagttttgta aggtccgatc ataaaaacca ccgcccattc ccaggcgctg gccgtactca
    14701 tcaaaggcga ccagcggtgt gatcaacacg tctaatcggg aaagggggag cacgtcacgc
    14761 acatccagtt ttggctcatg gatcttcaac ctgttcatca ccagttcgct ttgcggatgg
    14821 taattcagga acagcaaatt accggcacta aagggatgca aaactggaag atatacgcgc
    14881 ttaccggcgc gccagagttg ttctataagt ggctgggtgt cgagttcgcc atcaaaagag
    14941 aggaatacag cgaccgtatg cgccatcacc actgggggat aagtcatcat ccgggtagca
    15001 gcttgttgac ccatttcctg ctgttgttcc ggcgttaacg cacgacgacg ttgccgaatc
    15061 attttgcgga tttcttgtcg ggataatgtc agtgggagtt ctggtagttg cgtcatggct
    15121 ggtgccagat aagaagggaa tctccgagat gccgccgcag gctgtaaccc ttgaaccctt
    15181 ggttcaaggt gaatgtgtcg tcgcagtttt aaggcttctc ggacggaccg agcatgctca
    15241 ccaaccgcgg agcgccacat tcttgtggta tgaaatatcg gctcagggga ctggcccgct
    15301 tgcgaacatc tcagagaaat tttgtcttca cggttactct accacagtaa accgaaaagt
    15361 gtcattcaaa gttttggtta gttttttcgg tgatgcgacc ttgttcaagt aacgcttgtt
    15421 ctatggtctg ctgcagcatc cgaatacgct gttccatact tgccgcgtag tcacgagtct
    15481 tcgctttttc ttgcgctaac tcatagctga tattcaatgc ggcaatgaag accaactgtt
    15541 cagtatttgt gactctagtg cgttctttca gatcttgcaa ccgttggttc agatcgtccg
    15601 ctgcctgatt caacgcatcc ctttggtcag gcgggcagtt cacacgcagt gaacggccaa
    15661 aaatttggat atcgacgggt tgtgcagaca tgccaccttc ctgctgattg actgcgctgc
    15721 cttcgtcttt agacccatgg tctgcgaagg ggcgacacta tagctaccct gatgagaaga
    15781 gacaagccct tttctggtcc accagggccc aaagtggtag catatcatga atattcctcc
    15841 ctttgacgac gaatgcttat gtctatacag aacgaaatgc ctggttacaa cgaaatgaac
    15901 cagtatctga accaacaagg gacgggtctg acccctgctg agatgcatgg tttaatcagt
    15961 gggatgatat gtggcggtaa cgatgacagc tcatggctgc cgctacttca cgacctgacg
    16021 aacgaaggca tggctttcgg tcatgagctg gcacaggcac tgcgtaaaat gcactctgcc
    16081 accagcgatg ccctgcagga tgacggcttc ctttttcagc tttatctgcc tgatggcgat
    16141 gatgtcagcg ttttcgatcg ggctgatgcg ctggcaggtt gggtcaatca cttcctgctt
    16201 ggtcttggcg ttacgcaacc gaagctggac aaagtgaccg gcgaaaccgg tgaagccatc
    16261 gacgatctgc gtaacatcgc gcagctgggc tacgacgaag acgaagatca ggaagagctt
    16321 gaaatgtcgc ttgaagagat catcgaatac gttcgtgttg ccgcgctgtt atgccacgac
    16381 acctttactc atccgcaacc gaccgcgcca gaagtacaaa aaccgactct acactaaaaa
    16441 caaaaaacgt aaggagagtg ttatgagtga gatatcccgg caagagtttc agcgtcgccg
    16501 tcaggccctg gtggagcaaa tgcaacccgg cagcgccgcg ctgatttttg ctgcaccaga
    16561 agtaacacgt agcgccgaca gcgaataccc ctatcgtcag aacagtgact tctggtactt
    16621 caccggcttt aacgaaccgg aagcggtgct ggtgctgatt aaaagcgatg acactcataa
    16681 ccacagcgtt ttgtttaacc gcgttcgcga cctgacggca gagatctggt ttggtcgtcg
    16741 cttaggtcag gatgccgcgc cagagaaact gggtgttgat cgcgctctgg cattcagcga
    16801 aattaatcag caactttatc aactacttaa tggtctggat gtggtttacc acgcccaggg
    16861 cgaatatgca tatgctgatg aaatcgtgaa cagtgcgctg gaaaaactgc gtaaaggctc
    16921 gcggcaaaat cttaccgcac cggcaacgat gatcgactgg cgtcccattg ttcatgaaat
    16981 gcgcctgttc aaatcgccgg aagagattgc cgtacttcgc cgcgcggggg aaattaccgc
    17041 cctggcgcat acccgggcga tggaaaaatg ccgtccggga atgttcgagt accatctgga
    17101 aggcgaaatt caccacgaat ttaaccgcca cggtgcgcgt tatccgtcct acaacaccat
    17161 cgtcggcagc ggtgaaaacg gctgcattct gcactacacc gaaaacgaga gtgaactgcg
    17221 cgacggtgac ctggtgttga ttgacgcggg ttgtgaatac aaaggttacg cgggcgatat
    17281 tacccgcacc ttcccggtca acggcaaatt cactcaggcc cagcgtgaaa tctacgacat
    17341 tgtgctggag tctcttgaaa ccagcctgcg cctgtatcgt ccgggaactt ccatgcagga
    17401 agtcactggt gaagtggtgc gtatcatggt tagcggcctg gtaaaactcg gcatcctgaa
    17461 aggtgatgtt gatgaactga tcgctcagaa cgcccatcgt cctttcttta tgcatggcct
    17521 tagccactgg ttaggactgg atgtccatga cgttggcgtt tatggtcagg atcgctcgcg
    17581 cattctggaa ccggggatgg tactgaccgt agagcctggt ctgtatatcg cgccggatgc
    17641 ggatgtgcca gaacaatatc gcggtatcgg cattcgtatt gaagacgaca ttgtgattac
    17701 cgaaaccggc aacgaaaacc tcaccgccag cgtggtgaaa aagccggaag aaatcgaagc
    17761 gttgatggct gctgcgagaa agcaatgagc gtaatcatcg tcggtggcgg catggcgggc
    17821 gcgacgctgg cgctggctat ttcccggtta agtcacgggg cgctgccggt acatttgatt
    17881 gaagcgactg cgccagagtc acatgctcat ccgggctttg atggacgagc tattgcgctg
    17941 gcggcgggta cctgtcagca actggcgcgc atcggcgttt ggcaatctct ggcggattgc
    18001 gcaaccgcca tcaccagcgt gcatgtcagc gatcgtggtc acgctggatt tgtcaccctc
    18061 gccgcagaag attaccaact ggcggcgctg ggacaggttg tcgaattgca caatgtcggg
    18121 caacggctgt ttgcattgct gcgtaaagca cctggcgtaa cgctgcattg ccctgatcgc
    18181 gtggctaacg ttgcccgtac tcagagtcac gttgaagtga cgctggagtg tggcgaaacg
    18241 ctgacgggcc gcgtgctggt agccgctgat ggcacccatt cagcgttagc cactgtgtgc
    18301 ggcgttgact ggcagcagga gccttacgaa caactggctg tgattgccaa cgttgcgact
    18361 tccgttgcgc acgaagggcg cgcttttgaa cgctttacgc aacatggccc gctggcgatg
    18421 ttgccgatgt ctgacggacg ctgttcgctg gtctggtgtc atctactgga acggcgcgaa
    18481 gaggtactgt cgtggagcga cgagaagttt tgccgtgaac tccagtcggc ctttggttgg
    18541 cgacttggga aaattaccca cgctggtaaa cgcagtgctt atccgctggc gttaacccgc
    18601 gccgccagac ctattaccca tcgtaccgtg ctggtgggca atgcggcgca aactctgcac
    18661 ccgattgccg ggcaagggtt caacctcggt atgcgggatg tcatgagcct tgcggaaacc
    18721 ttgactcagg cgcaggagcg cggagaagac ataggtgatt acggcgtatt gtgccgttat
    18781 cagcagcgtc gacagagcga tcgcgaagca accatcggtg tcacggacag ccttgtacat
    18841 ctttttgcca accgttggac gccgctggtt gtcgggcgca acatcgggct gatgacgatg
    18901 gaattattta ccccggcacg cgatgtgctg gcgcagcgca ccctcggttg ggtggcgcgt
    18961 tgaaggcggt tttaaggaat aaacaatgca aagtgttgat gtagccattg ttggcggcgg
    19021 catggtgggg ctggcggttg cctgcggctt acaggggagc ggcttacgcg ttgccgtact
    19081 ggagcagcgc gtaccggaac ctctggcggc ggatgcacca ccacaactgc gcgtttcggc
    19141 tatcaatgcc gccagcgaaa aattactcac ccgtcttggc gtctggcagg acattctctc
    19201 tcgtagggcc agttgttatc acggtatgga agtgtgggac aaagacagtt ttggtcacat
    19261 ttcgtttgac gatcaaagca tgggctatag ccatcttggg catatcgttg aaaactcagt
    19321 gattcactac gcgctgtgga acaaagcgca gcagtcgtca gatatcactc tgttggcccc
    19381 cgcagaatta cagcaggtcg cctggggaga aaatgaaacc ttcctgacgc tgaaagatgg
    19441 cagcatgtta acggcgcgtc tggtgattgg cgcggacggc gctaattcct ggttgcgcaa
    19501 caaagccgat attccgctga ctttctggga ttatcagcat cacgcgctgg tagcgaccat
    19561 tcgcacggaa gaaccgcatg atgcggtggc gcggcaggtt ttccatggcg aaggcattct
    19621 ggccttttta ccgcttagcg atccgcatct ttgctcgatt gtctggtcac tgtcgccaga
    19681 ggaagcgcag cggatgcagc aggcaagtga agacgaattt aatcgcgcgt taaatatcgc
    19741 ttttgataat cgccttggct tatgcaaggt tgagagtgcg cgtcaggtgt tcccactgac
    19801 ggggcgttat gcgcgccaat ttgccgcgca ccgtctggcg ttggtgggcg acgccgcgca
    19861 taccattcac ccgctcgcgg ggcagggcgt taatctcggc tttatggatg ccgcagagct
    19921 gattgccgaa ctgaaacggt tgcatcgtca ggggaaagac atcgggcagt acatttatct
    19981 gcgtcgctat gagcgtagcc gcaagcacag tgcggcgctg atgctggctg gtatgcaggg
    20041 attccgcgat atgttttccg gtaccaatcc ggcgaaaaaa ctgctgcgtg atattggttt
    20101 gaaactggcc gacacgcttc ctggcgttaa accacaactt atccgccagg caatgggatt
    20161 aaacgatttg cctgaatggc tgcgttaaaa atttctcttc tgctgtttat ttgataccca
    20221 tcacactttc atctcccggt tttttcgccg ggagattttc ctcatttgaa ataaactaat
    20281 ttcacctccg ttttcgcatt atattttcta atgccacgaa tttttgattt agtgtttttt
    20341 gacatttttt cagctcttaa tattgcctta ttcaaattaa tttcctcatc gcatcatctt
    20401 tctatagaaa ttggtgtgtt ttttggtttt ttattctgtt gtgattttgg cgttttttaa
    20461 ccataagcta atgtgatgat caattttacc ttatggttaa cagtctgttt cggtggtaag
    20521 ttcaggcaaa agagaacgat tgcgttgggg accgggagtg gctccgatgc tgggtttcgt
    20581 ggtgataatt tcgccatgga aaagttgtca gccccgctta ttcaatgagg acaagatggc
    20641 acaacagact cctttgtacg aacaacacac gctttgcggc gctcgcatgg tggatttcca
    20701 cggctggatg atgccgctgc attacggttc gcaaatcgac gaacatcatg cggtacgtac
    20761 cgatgccgga atgtttgatg tgtcacatat gaccatcgtc gatcttcgcg gcagccgcac
    20821 ccgggagttt ttgcgttatc tgctggcgaa cgatgtggcg aaactcacca aaagcggcaa
    20881 agccctttac tcggggatgt tgaatgcctc tggcggtgtg atagatgacc tcatcgtcta
    20941 ctactttact gaagatttct tccgcctcgt tgttaactcc gccacccgcg aaaaagacct
    21001 ctcctggatt acccaacacg ctgaaccttt cggcatcgaa attaccgttc gtgatgacct
    21061 ttccatgatt gccgtgcaag ggccgaatgc gcaggcaaaa gctgccacac tgtttaatga
    21121 cgctcagcgt catgcggtgg aagggatgaa accgttcttt ggcgtgcagg cgggcgatct
    21181 gtttattgcc accaccggtt acaccggtga agcgggctac gaaattgcgc tgcccaatga
    21241 aaaagcggcc gatttctggc gcgcgctggt ggaagcgggc gttaagccat gcggtctggg
    21301 cgcgcgtgac acgctgcgtc tggaagcggg catgaatctt tatggtcagg agatggacga
    21361 aaccatctct ccgttagccg ccaacatggg ctggactatc gcctgggaac cggcagatcg
    21421 tgacttcatc ggtcgtgaag ccctggaagt gcagcgtgaa catggcacag aaaaactggt
    21481 tggtctggtg atgactgaaa aaggcgtgct gcgtaatgaa ctgccggtac gttttactga
    21541 tgcgcagggc aaccagcatg aaggcattat caccagcggt actttctccc cgacgctggg
    21601 ttacagcatt gcgctggcgc gtgtgccgga aggtattggt gaaacggcga ttgtgcaaat
    21661 tcgcaaccgt gaaatgccgg ttaaagtgac gaaacctgtt tttgtgcgta acggtaaagc
    21721 cgtcgcgtga tttacttttt tggagattga ttgatgagca acgtaccagc agaactgaaa
    21781 tacagcaaag aacacgaatg gctgcgtaaa gaagccgacg gcacttacac cgttggtatc
    21841 accgaacatg cccaggagct gttaggcgat atggtgtttg ttgacctgcc ggaagtgggc
    21901 gcaacggtta gcgcgggcga tgactgcgcg gttgccgaat cggtaaaagc ggcgtcagac
    21961 atttatgcgc cagtaagcgg tgaaatcgtg gcggtaaacg acgcactgag cgattccccg
    22021 gaactggtga acagcgaacc gtatgcaggc ggctggatct ttaaaatcaa agccagcgat
    22081 gaaagcgaac tggaatcact gctggatgcg accgcatacg aagcattgtt agaagacgag
    22141 taacggcttt attcctcttc tgcgggagag gatcagggtg aggaactttt acgcctcacc
    22201 ctcactctct tcgcaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct
    22261 catgacacag acgttaagcc agcttgaaaa cagcggcgct tttattgaac gccatatcgg
    22321 accggacgcc gcgcaacagc aagaaatgct gaatgccgtt ggcgcacaat cgttaaacgc
    22381 gctgaccggc cagattgtac cgaaagatat tcagcttgcg accccgccgc aggttggcgc
    22441 accggcgacc gaatacgccg cgctggcgga actcaaggct attgccagtc gcaataaacg
    22501 cttcacgtct tacatcggca tgggttacac cgccgtgcag ctaccgccgg ttattctgcg
    22561 taacatgctg gaaaatccgg gctggtatac cgcgtatact ccgtatcaac ctgaagtctc
    22621 ccagggccgc cttgaggcac tgctcaactt ccagcaggta acgctggatt tgactggact
    22681 ggatatggcc tctgcttctc ttctggacga ggccaccgct gccgccgaag caatggcgat
    22741 ggctaaacgc gtcagcaaac tgaaaaatgc caaccgcttc tttgttgctt ctgacgttca
    22801 tccgcaaacg ctcgatgtgg tccgtactcg tgctgaaacc tttggttttg aagtgattgt
    22861 cgatgacgcg caaaaagtgc tcgaccacca ggacgtcttc ggcgtgctgt tacagcaggt
    22921 cggcactact ggtgaaattc acgactacac cacgcttatt agcgaactga aatcacgcaa
    22981 aattgtggtc agtgtcgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa
    23041 acagggcgcg gatattgttt ttggttcggc gcaacgcttt ggcgtaccga tgggctacgg
    23101 tggcccacac gcggcattct ttgcggcgaa agacgaatac aaacgctcaa tgccgggccg
    23161 tattatcggt gtatcgaaag atgcagctgg caacaccgct ctgcgcatgg cgatgcagac
    23221 tcgcgagcaa catattcgtc gtgagaaagc gaactccaac atttgtactt cccaggtgct
    23281 gctggcaaac atcgccagcc tgtatgccgt ttatcacggc ccggttggcc tgaaacgtat
    23341 cgctaaccgc attcaccgtc tgaccgatat cctggcggca ggcctgcaac aaaaaggtct
    23401 gaagctgcgc catgcgcact atttcgacac tctgtgtgtg gaagtggccg acaaggcggg
    23461 cgtactggcg cgtgccgaag cggctgaaat caacctgcgt agcgatatcc tgaacgcggt
    23521 tgggatcacc cttgatgaaa ccaccacgcg cgaaaacgtg atgcagcttt tcagcgtact
    23581 gctgggggat aaccacggac tggacatcga cacgctggac aaagacgtgg ctcacgatag
    23641 ccgctctatt cagcctgcaa tgttgcgcga cgacgaaatc ctcacccatc cggtgtttaa
    23701 tcgctaccac agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct
    23761 ggcgctgaat caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc
    23821 cgagatgatc cccatcacct ggccggaatt tgccgaactg cacccgttct gcccgccgga
    23881 acaggccgaa ggttatcagc agatgattgc gcaactggct gactggctgg tgaaactgac
    23941 cggttacgac gccgtttgta tgcagccgaa ctctggcgca cagggcgaat atgcgggcct
    24001 gctggcgatt cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcttgat
    24061 cccggcttct gcgcacggaa ctaaccccgc ttctgcacat atggcaggaa tgcaggtggt
    24121 agttgtggca tgtgataaaa atggcaacat cgatctgact gacctgcgcg cgaaagcgga
    24181 acaagcgggc gataatctct cctgcatcat ggtgacttat ccttccaccc acggcgtgta
    24241 cgaagaaacg atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct
    24301 tgatggcgcg aacatgaacg cccaggtcgg catcacctca ccgggcttta ttggcgcgga
    24361 cgtttcgcac cttaacctgc ataaaacttt ctgcattccg cacggtggcg gcggcccagg
    24421 tatgggaccg atcggcgtga aagcgcatct ggcaccgttt gtgccaggtc atagcgtggt
    24481 acaaattgaa ggcatgttaa cccgtcaggg cgcggtttct gcggcaccgt tcggtagcgc
    24541 atccattctg ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa
    24601 agcaagccag gtggcaattc ttaacgccaa ctatattgcc agccgcctgc aggatgcttt
    24661 cccagtgctg tataccggtc gcgacggtcg cgtggcgcac gaatgtattc ttgatattcg
    24721 tccgctgaaa gaagaaaccg gcatcagcga actggatatt gccaagcgtc tgatcgacta
    24781 cggtttccac gcgccgacga tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac
    24841 tgaatctgaa agcaaagtgg aactggatcg ctttatcgac gcgatgctgg ctatccgcgc
    24901 agaaatcgac caggtgaaag ccggagcctg gccgctggaa gataacccgc tggtgaacgc
    24961 gccgcacatt cagagcgaac tggtcgccga gtgggcgcat ccgtacagcc gtgaaattgc
    25021 ggtattcccg gcaggcgtgg cagacaaata ctggccgaca gtgaaacgtc tggatgatgt
    25081 ttacggcgac cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc agtaatttac
    25141 tgattcgact atcttctaaa ggcgcttcgg cgccttttta gcttgttaat aaagtgcgct
    25201 ttgtgtgtcg gatgaagcgt aaatataaac tcctggagtt caatatatga caggagcgcc
    25261 aggcctgatg agcggggcgc atcaggcaca tcgggtaatt aaagcttctc gccgttgctg
    25321 gcaatcacct ctttgtacca gtcaaagctc ttcttacgtg aacgcgacat atcgccagta
    25381 ccgtcgtcat gtttattcac atagataaag ccgtagcgtt tgctgtactg cccggtagtg
    25441 aacgacacgc agtcgatgca gccccacggt gtgtagccca tcagatccac gccatcgtaa
    25501 gtcaccgctt ttttcatctc ttcaatatgg gcgcgcaggt agtcaatgcg gtagtcgtcg
    25561 ttgatgctgc catcttcttc catcttgtcg taagcgccaa aaccgttttc gacaataaac
    25621 agcggcttct gataacgctc atacagttcg caaagtgcat agcgcagacc cacaggatca
    25681 atctgccagc cccagtcaga cgctttaaca tacgggtttg gtacgctgcc ttcaaaacca
    25741 gagatcgcat cgccggtgcc gccttcggcc ttcaccgcgt tggtcatgta atagctgaaa
    25801 ccgagataat cgcaggtgcc ttcgcgcagc acatcgagat cgccgtcttc cattttgatg
    25861 ttaaatccgc gacgctccca ctcgttcaac acataggacg ggtaatagcc acgcagttgc
    25921 acatcggtaa agacgtagcg ttcgcgcatc gactcctggg cgaacatcac atcgtccgga
    25981 ttacaggagt aagggtaaag cggcaccatc gccagcatac agccgacttt catttccggg
    26041 ttaatgcgac gcgcggcttt caccgccagg gcgctggcga caaactggtg atgcagcacc
    26101 tgatacatcg tctcttccgg gttctcatgc tcggtataaa ccacgccgga gcagcagtaa
    26161 ccgaacagcg gtgcacgcca gttacgctgg ttgttaattt cgttgaaggt catccagtat
    26221 ttgactttat acttatagcg ttcaaatacc acttccgcga aacggacaaa gaaatcaacc
    26281 actttacggt tggtccagct accgtattgc tgcaccagat gcagcggcat ttcaaagtgg
    26341 gagagggtga tcactggttc gatgttgtat ttcagcagtt catcgaacat atcatcgtag
    26401 aacttcagcc cttcttcgtt tggctgagct tcatcacctt ttggaaaaat gcgcgtccag
    26461 gcgatggatg tacgaaaaca tttgaagccc atttcggcaa atagcttgat gtcttccttg
    26521 taatgaccat aaaaatcaac ggcttcatgg ttcggatagt attttcctgg caacacttct
    26581 ttggtgattt cgcgcggcac gccgtgtgcg ccaccggtca gaacgtcaca aatgctcggc
    26641 cctttgccgc ctttgttcca gccgccttcg acctgatgag cggcaactgc gccgccccat
    26701 aagaaatctt tcggtaaggt gagttttttc actatcatag gctccagatg agtcaattct
    26761 tacaggaaat agtaccatgc agccaaacga catcactttt tttcaacgtt tccaggatga
    26821 cattctggct gggcgtaaaa ctatcaccat ccgcgacgag tctgaatcgc acttcaaaac
    26881 gggtgatgtg cttcgtgtcg ggcgttttga agatgacggt tatttttgca cgattgaagt
    26941 caccgcaacc tcaaccgtaa cgctggatac gctgacggaa aaacatgcag agcaggagaa
    27001 tatgaccctg ctagaactga aaaaggtcat tgccgacatc tatcccggcc agacacagtt
    27061 ttatgtgatt gaatttaaat gcctttaaat tgaactcacg cctgttagtt gaaattttgc
    27121 atttaatttc atgattttta ggagattagt cccaaatcag aatttatttt agctaacagg
    27181 tgttcactgg aactattctc agttacgctg gagaggtata catgtgcgtg tacgtttctc
    27241 cggagtaagt tatggttcag aagcccctca ttaagcaggg atattcgctg gcagaggaaa
    27301 ttgccaacag cgtcagtcac ggcattgggt tggtgtttgg tatcgttggg ctggtgttgc
    27361 tactggttca ggcggtggat ctcaatgcca gcgccacagc gataaccagt tacagcctct
    27421 atggcggcag tatgatcctg ctgttcctcg cttcgacgct ctatcacgcc attcctcatc
    27481 aacgggcaaa aatgtggctg aagaaatttg accattgcgc tatttaccta ttgattgccg
    27541 gaacctacac gccgtttttg ctggtggggc tggattctcc gttagcgcgc gggttgatga
    27601 ttgttatctg gagcctggca ttgctgggga ttctgttcaa actgaccatc gcgcaccgat
    27661 tcaaaatttt atctctggtg acctatctgg cgatgggctg gctgtcgctg gtggtgattt
    27721 atgaaatggc aattaagctt gcggcgggca gcgttacctt actggctgta ggcggcgtgg
    27781 tttactcgct cggggtgatt ttctacgtct gcaaacgcat tccctacaac catgccatct
    27841 ggcacggctt tgtgctcggc ggcagcgtgt gccactttct ggcgatctat ttatatattg
    27901 ggcaggcgta atgggtgtgc ctttgtaggc ctgaaaagac acacccttgt cgcatcaggc
    27961 gataaatgca tgatgcgaca cagagattta ctcttcgagt gaatacggca gcggctcgat
    28021 atgcaatgta ttcgcatcgt cacgcacgcg gaagatgctg tccggttcca tatcgttatt
    28081 catgacgacc tgtaccacga cctgaccatc ttccagtttt accgcagcca gcacggttcc
    28141 ggtacgacgc cagttctcgc ccattttcag ctctaagtct tcaccggctt ccggcagtcg
    28201 gctggcgcta cccgtcagca accagagcgc gcgtttgttg gcaccacgga atttagctcg
    28261 cgccaccatc tcttgtccgg tatagcagcc ttttttaaag ctaatgccgc ccagcgcctg
    28321 gaggttggtc gcctgtggga taaactgccc gctgttggcg gcatcaatca ccgggaaacc
    28381 cgcttcaatg tttaatgcca gccactgttg gctattgttc agttccgctt caccgcgcag
    28441 tttatcggtc agcatattgg cagtggcttc atcggttacg atcaggaaac gttctgcagg
    28501 gtgttcaaac catagcaaag tggtcgcgcc ttctttaact acctgttttt ctttcgaagg
    28561 cagttcacta aagatatttg ccagcgcggc gcgcgcctga aaaccggcaa caccaagcag
    28621 cacacgctca tcgtccggcg cgatggtcac tttagagaac accgcatatt ttttcagttc
    28681 agtcagctgc ggttcacgca cactgcgccg ttcaatccat gcaaagccat cgccgtcgcg
    28741 gaacagacgt aaattgctcc acattttacc tttggcgtcg caatgggcgg cgagcagatg
    28801 ctgattttct gtcatctggc tgacatctgc tgtcacctga ccctgcatat atttttcgct
    28861 gtccgcgcca gtaatggtgg caagcgccca gtcatcaagc gttatcagcg tcagcggtaa
    28921 acgggcagac gccgtaggct gacggggagg aaaaggtgta aaagccataa tagagtcctg
    28981 agttgcttaa cggcgtatta atgactaatg gtaaaagagc tatgaggcaa tgcaagtgct
    29041 ttcactacgc gatctgtgcc ttctgcgagg cgacttccaa aaaaatgaag cccttgaatt
    29101 gttgtgaatt aatttcgcga tcgcgattgt gtttactgat attcccgcaa aagaagtcga
    29161 aaaacacgtt acaatagcca ggtactggat tttcgtgaga caggaagaag atggacatta
    29221 acaacaaagc ccgcattcat tgggcatgcc gccgtggtat gcgcgaactc gatatttcaa
    29281 tcatgccgtt tttcgaacat gagtacgaca gcttaagcga tgacgaaaaa cgcatcttta
    29341 ttcgtctgct ggagtgtgac gatcccgatc tgtttaactg gctgatgaac cacggtaaac
    29401 cggccgatgc agaactggaa atgatggtcc gactcatcca gacacggaac cgggaacgtg
    29461 gtcctgtggc aatctgatct gcgcgtctcc tggcgcgcac agtggctttc cttgctgatc
    29521 cacgggctgg ttgccgctgt tattttactc atgccctggc cgctcagtta caccccgtta
    29581 tggatggtgt tactttcgct ggtggtgttt gattgcgttc gcagccagcg gcgcattaat
    29641 gctcgccagg gggaaattcg cctgttgatg gacgggcgtt tgcgctggca agggcaggag
    29701 tggagcatcg tcaaagcgcc gtggatgatt aagagcggca tgatgctgcg tttacgttct
    29761 gacagcggta aacggcaaca tttatggctg gcagccgaca gcatggacga agccgaatgg
    29821 cgggatttac ggcggatatt gttgcaacaa gagacgcaaa gataaagatg acggcaaagc
    29881 atatgcgcta agccgtcagt gacatcaggc gtaatgctct gccatttcgt tgaggatttg
    29941 ctcgcaccag ttctgaatac gctcgtcgct aaggtcatat tggttagttt cgtccagcgc
    30001 cagacccacg aacagttgcc cgtcagcaat caccggtttc gggctggtaa actcatatcc
    30061 ttccgttggc cagtagccga cgaacttcac gcctttggtc gagagcttgt catgcagtat
    30121 accgagcgca tcgaggaacc actcgccgta tcccagttga tcgccaagcc catacagcgc
    30181 aacaatttta ccttcgaggt tcaggtcgtc gagctgatcc cagacggctt cccagtcttc
    30241 ctggatttca ccaaaatccc aggtcgggat acccagaatg agcacatcgt actgctccat
    30301 taatttcggg gagtcgtcct tgaggttatg taaggtcacc agttctgggc cgataatatc
    30361 gcggattttt tctgccgcca tttcggtgta acaggtgctg gaaccgtaaa aaagacccat
    30421 attcataacg taagcttctc gattctgctg tggcttttgt ggctagtgta tcagaatcgc
    30481 tttagtcacg gcacaataag gcataatgga tcaaatgagt gaccataagg ggcgcaagtg
    30541 aaacaggatc tggcacgcat cgagcagttt cttgatgctc tgtggctgga aaaaaatttg
    30601 gctgaaaata cgttgaacgc ttaccgtcgc gatctgtcaa tgatggtgga gtggttgcat
    30661 caccgcgggt tgacgctggc gacggcgcaa agtgacgatt tgcaggcatt actggcagaa
    30721 cggctggagg gcgggtataa agccaccagc tcagcgcgtt tgctgagtgc agtgcgccga
    30781 ttgttccagt atctttatcg cgaaaagttt cgtgaagacg atcccagtgc gcatcttgct
    30841 tcaccgaaat tgccccagcg tttgccaaaa gatttaagcg aagcgcaggt cgaacgttta
    30901 ttacaggcac cattaattga tcagccactg gagctacgcg ataaagccat gcttgaagtg
    30961 ttgtatgcta ccgggctgcg tgtctctgaa ctggtcgggc tgacaatgag tgatatcagc
    31021 ctgcgtcagg gcgtagtgcg ggtcattggt aaaggcaaca aagagcgcct ggtgccgtta
    31081 ggtgaagagg cggtttactg gctggaaacg tatctcgagc atgggcgacc gtggttgttg
    31141 aacggtgtct ctattgatgt gttgttcccc agccagcgtg cgcagcagat gacgcgacag
    31201 accttctggc accgcattaa acattatgcc gtgctggcgg gtatcgacag cgaaaagctg
    31261 tcaccgcatg tcttgcgtca cgcttttgcc actcatttat taaatcatgg tgcggattta
    31321 cgcgtggtgc agatgctact gggccacagc gatctctcca ccacgcaaat ttatacgcat
    31381 gtcgctaccg agcgtctgcg gcaacttcat caacagcatc acccgcgggc gtgatgtctg
    31441 aaaagaacgg gaagatttat gaagaaaggt tttatgttgt ttactttgtt agcggcgttt
    31501 tcaggctttg ctcaggctga tgacgcggca attcaacaaa cgttagccaa aatgggcatc
    31561 aaaagcagcg atattcagcc cgcgcccgta gctggcatga agacagttct gactaacagc
    31621 ggtgtgttgt acatcaccga tgatggtaaa catatcattc aggggccaat gtatgacgtt
    31681 agtggcacag ctccggtcaa tgtcaccaat aagatgctgt taaagcagtt gaatgcgctt
    31741 gaaaaagaga tgatcgttta taaagcgcca caggaaaaac acgtcatcac cgtgtttact
    31801 gatattacct gtggttactg ccacaaactg catgagcaaa tggcagacta caacgcactg
    31861 gggatcaccg tgcgttatct tgctttccca cgccaggggc tggagagcca ggctgagcaa
    31921 caaatgaaag ccatctggtg tgcgaaagat aaaaacaaag cgtttgatga tgtgatggct
    31981 ggtaaagctg ccactccggc aagttgcgat atcgatattg ccgatcatta cgcacttggc
    32041 gtccagcttg gcgttagcgg cactccggca attgtgctga gcaatggcac acttgttccg
    32101 ggttaccagc caccgaaaga catgaaagaa ttcctcgacg aacaccaaaa aatgaccagc
    32161 ggtaaataat tcgcgtgaaa caacagatac aacttcgtcg ccgtgaagtc gatgaaacgg
    32221 cagacttgcc cgctgaattg cctcccttgc tgcgccgttt atacgccagc cggggagtac
    32281 gcagtgcgca agaactgaaa cgcagtgtta aaggtatgtt gccctggcag caactgagcg
    32341 gcgtcgaaaa ggccgttgag atcctttaca acgcttttcg cgaaggaacg cggattattg
    32401 tggtcggtga tttcgacgcc gacggcgcga ccagcacggc tctaagcgtg ctggcgatgc
    32461 gctcgcttgg ttgcagcaat atcgactacc tggtaccaaa ccgtttcgaa gacggttacg
    32521 gcttaagccc ggaagtggtc gatcaggccc atgcccgtgg cgcgcagtta attgtcacgg
    32581 tggataacgg tatttcctcc catgcggggg ttgaacatgc tcgctcgttg ggcatcccgg
    32641 ttattgttac cgatcaccat ttgccgggcg acacattacc cgcagcggaa gcgatcatta
    32701 accctaactt gcgcgactgt aatttcccgt cgaaatcact ggcaggcgtg ggtgtggcgt
    32761 tttatctgat gctggctctg cgcacttttt tgcgcgatca gggctggttt gatgagcgcg
    32821 gcatcgcaat tcctaatctg gcagaactgc tggatctggt cgcgctggga acagtggcgg
    32881 acgtcgtgcc gctggacgct aataatcgca ttctgacgtg gcaggggatg agtcgcatcc
    32941 gtgccggaaa gtgccgtccg gggattaaag cgctgctgga agtggccaac cgtgatgcac
    33001 aaaaactcgc cgccagcgat ttaggttttg cgctggggcc acgtctcaat gctgccgggc
    33061 gactggatga tatgtccgtt ggtgtggcac tgttgctgtg cgacaacatc ggcgaagcgc
    33121 gcgtgctggc aaatgaactc gatgcgctaa accagacgcg aaaagagatc gaacaaggaa
    33181 tgcaggttga agccctgacc ctgtgcgaga aactggagcg cagccgtgac acgctacccg
    33241 gcgggctggc aatgtatcac cctgaatggc atcagggcgt tgttggcatt ctggcatcgc
    33301 gtatcaaaga gcgttttcac cgtccggtta tcgcctttgc ccctgcgggc gacggtacgc
    33361 tgaaaggttc tggtcgttcc attcaggggt tgcatatgcg cgatgcgctg gagcggctgg
    33421 acacgctcta ccccggtatg atgcttaagt ttggcggcca tgcaatggcg gccgggctgt
    33481 cactggaaga ggataaattc gaactctttc agcaacgctt tggcgaactg gtgactgagt
    33541 ggctggaccc ttcactattg caaggcgaag tggtgtcaga cggtccgtta agcccggccg
    33601 aaatgaccat ggaagtggcg cagttactgc gcgatgctgg cccgtgggga cagatgttcc
    33661 cggaaccgct gtttgacggt catttccgcc ttctgcaaca acgactggtg ggcgagcgcc
    33721 atttgaaggt catggttgaa ccggtcggcg gcggcccgct gctggacggc attgccttta
    33781 acgttgatac cgccctttgg ccggataacg gcgtgcgcga agtgcaactg gcttataagc
    33841 ttgatatcaa cgagtttcgc ggcaatcgca gcctgcaaat tatcatcgac aatatctggc
    33901 caatttagcg tcatcttctc tataaaaaag agtgtggatt gggtacaatc ccgctcttat
    33961 caccgcattt tgactagctc aagaaaagaa atcagaccat gtttgaaatt aatccggtaa
    34021 ataatcgcat tcaggacctc acggaacgct ccgacgttct tagggggtat ctttgactat
    34081 gacgccaaga aagagcgtct ggaagaagta aacgccgagc tggaacagcc ggatgtctgg
    34141 aacgaacccg aacgcgcaca ggcgctgggt aaagagcgtt cctccctcga agccgttgtc
    34201 gacacccttg accaaatgaa acaggggctg gaagatgttt ctggtctgct ggaactggct
    34261 gtagaagctg acgacgaaga aacctttaac gaaaccgttg ctgaactcga cgccctggaa
    34321 gaaaaactgg cgcagcttga gttccgccgt atgttctctg gcgaatatga cagcgccgac
    34381 tgctacctcg atattcaggc ggggtctggc ggcacggaag cacaggactg ggcgagcatg
    34441 cttgagcgta tgtatctgcg ctgggcggag tcgcgtggtt tcaaaactga aatcatcgaa
    34501 gagtcggaag gtgaagtggc gggtattaaa tccgtaacga tcaaaatctc cggcgattac
    34561 gcttacggct ggctgcgtac agaaactggc gttcaccgcc tggtgcgtaa gagcccgttt
    34621 gactccggcg gtcgtcgcca cacgtcgttc agctccgcgt ttgtttaccc ggaagttgat
    34681 gacgatatcg atatcgaaat caatccggcg gatctgcgca ttgacgttta tcgcgcgtcc
    34741 ggcgcgggcg gtcagcacgt taaccgtacc gaatctgcgg tgcgtattac tcacatcccg
    34801 accgggatcg tgactcagtg ccagaacgac cgttcccagc acaagaacaa agaccaggcc
    34861 atgaagcaga tgaaagcgaa gctttatgaa ctggagatgc agaagaaaaa tgccgagaaa
    34921 caggcgatgg aagacaataa atccgacatc ggctggggca gccagattcg ttcttatgtc
    34981 cttgatgact cccgcattaa agatctgcgt accggggtag aaacccgcaa cacgcaggcc
    35041 gtgctggacg gcagcctgga tcaatttatc gaagcaagtt tgaaagcagg gttatgagga
    35101 accaacatgt ctgaacaaca cgcacagggc gctgacgcgg tagtcgatct taacaatgaa
    35161 ctgaaaacgc gtcgtgagaa gctggcgaac ctgcgcgagc aggggattgc cttcccgaac
    35221 gatttccgtc gcgatcatac ctctgaccaa ttgcacgcag aattcgacgg taaagagaac
    35281 gaagaactgg aagcgctgaa catcgaagtt gccgttgctg gccgcatgat gacccgccgt
    35341 attatgggta aagcgtcttt cgttaccctg caggatgtgg gtggccgcat tcagctgtac
    35401 gttgcccgtg acgatctgcc ggaaggcgtt tacaacgagc agttcaaaaa atgggacctc
    35461 ggcgacatcc tcggcgcgaa aggtaaactg ttcaaaacca aaaccggcga actgtctatc
    35521 cactgcaccg agctgcgtct gctgaccaaa gcactgcgtc cgctgccgga caaattccac
    35581 ggcttgcagg atcaggaagc gcgctatcgt cagcgttatc tggatctcat ctctaacgat
    35641 gaatcccgca acacctttaa agtgcgctcg cagatcctct ctggtattcg ccagttcatg
    35701 gtgaaccgcg gctttatgga agttgaaacg ccgatgatgc aggtgatccc tggcggtgcc
    35761 gctgcgcgtc cgtttatcac ccaccataac gcgctggatc tcgacatgta cctgcgtatc
    35821 gcgccggaac tgtacctcaa gcgtctggtg gtcggtggct tcgagcgtgt attcgaaatc
    35881 aaccgtaact tccgtaacga aggtatttcc gtacgtcata acccagagtt caccatgatg
    35941 gaactctata tggcttacgc agattacaaa gatctgatcg agctgaccga atcgctgttc
    36001 cgtactctgg cacaggatat tctcggtaag acggaagtga cctacggcga cgtaacgctg
    36061 gacttcggta aaccgttcga aaaactgacc atgcgtgaag cgatcaagaa atatcgcccg
    36121 gaaaccgaca tggcggatct ggataacttc gactctgcga aagcgattgc tgaatctatc
    36181 ggcatccacg ttgaaaagag ctggggtctg ggccgtatcg ttaccgagat cttcgaagaa
    36241 gtggcagaag cacatctgat tcagccgacc ttcattactg aatatccggc agaagtttct
    36301 ccgctagcgc gtcgtaacga cgttaacccg gaaatcacag accgctttga gttcttcatt
    36361 ggtggtcgtg aaatcggtaa cggctttagc gagctgaatg acgcggaaga tcaggcgcag
    36421 cgcttcctgg atcaggttgc cgcgaaagac gcaggcgacg acgaagcgat gttctacgac
    36481 gaagattatg tcaccgcact ggaacacggc ttaccgccga cagcaggtct gggaattggt
    36541 atcgaccgta tggtaatgct gttcaccaac agccatacca tccgcgacgt tattctgttc
    36601 ccggcgatgc gtccggtaaa ataagcatta cgttatgctc acaaccccgg caaatgtcgg
    36661 ggttttttaa ttgtgctgcg cgaaagcaga caacaatttt cttgcttcac tattggctgc
    36721 ctgcatcacc atccacggac tgaacgccca cggcgtggca tcaataccgc gtaatacatc
    36781 tgctaaatca caccattgat aatccatcac ttcatcatcg ttgatctgta acgcactggt
    36841 ggtgcgtgcg gcaaataccg gacacacttc attttccaca atgccattcg gatcggtggc
    36901 gcgatagcga aagtcaggat agatagattc aggaggcgta atttccacgc caagctcata
    36961 acggcaacgg cggatcactg cgtcttcgtt gctttctccc tgttgtgggt gcccacaaac
    37021 cgagttagtc cacacgccag gccatgcttt tttgctcagt gcgcggcggg taactaataa
    37081 ttgccccttg gcattaaaca gccaactgga gaacgcgaga tgtaagaggg tgtctgccgt
    37141 gtgtgcggca tacttttcca gcgtacccgt gggaactccc tgtgcattca ataaaatgac
    37201 gtgttccgtt tgcataattt ctcacatgta attctaatca ctcatcatta tagataattg
    37261 aagagtgaaa tgattaacca agtgagcgtt tatcgacaac cgcccgtttt gagcggatgt
    37321 cgacaggtaa aaaccattta aaaaagcagc atcatatatt tgaagcgact ttcaatggcc
    37381 attgcaacac agtcagaaat gacaggaata gagagagtaa aaagcaacaa cagactgaat
    37441 atagcgcgca ttaaaagatt catattattc tccatgctca tttttcttca gcttgcgcat
    37501 cagaagccac atacgtactg tcctgacttt taaatcttta tcagagacgg attccttcac
    37561 cagatcagca tgatgctgat agccattaaa gaagacgttc agcacaacgg cgctaagggt
    37621 tgcgagcata atgccgctat gcagcaacgg ttgcagtacg gccggtaact tagaaaagaa
    37681 atcgtgagag agcgtcggag tcatgccaac gccgagactg atcgccacaa tatagaggtt
    37741 gtaacgattg gtggtgtagt tacagcgcga cagaattcga atccctgtcg ccagtaccat
    37801 gccgaacatc accagcccag caccgcccag cacaaattgc ggaatggagg cgaccagcac
    37861 cgccattttt ggcaccatgc caaacaggat taatataatt cccgaagaaa tacacaccca
    37921 gcggctatga acgcgcgtca cgctaaccag gccaacgttt tgagaaaatg acgtgtgggg
    37981 aaagctgtta aacgttccgc ctatcattgt ccctacgcca tcgacacgca gcccgcgaat
    38041 aatatcgtgt gaagagagtt tacgaccgac tatttcaccc agtgccagga acatccccat
    38101 cgactcgata aacacgatga ttaacacggc agtcatggtc agaatggaaa cgggatcaaa
    38161 aatcggcata ccaaacgaca tcggcgtaac aatcgcaaac cacgaggcat catgtagccc
    38221 ggataaattg acttcattca tcatccacga aagtaaaaag ccaaatacaa tccccagtaa
    38281 tacggcgacg ttggacataa atcctttcgc atagcgagta atgagcaaga tgaaaattaa
    38341 gacggcaaag gagataccta aataaacggg attaccatat tgcggattcc ctttacctcc
    38401 tgcggcccag tcaataccca cctgaatgat gctaagtccg atagaagtaa taaccacacc
    38461 ggtaaccagt ggcgggaata aaggcatcaa gcgaccgata agtggcgcta ataatgtggt
    38521 gataaaaccc gcggcgatag tggcaccaaa tatccccagc aggccgatat ccgggttcat
    38581 cccaatggct atcattggtg ttacagcagc aaaggtcacc gacataatca ccggcaggcg
    38641 gatccccata aagcggccga taccgataca ttgcaataat gtgacgatcc cgcagcaaaa
    38701 gagatccgag ctaatgagca tcgcaatagc ttcttttgag aggcccagtc ggtcaccaat
    38761 cataagagga acagcgactg cacctgcgta catgacaagg acgtgttgca gaccgagtat
    38821 gatcagcttt cctggtgata atatgcggtc aacctcatca gttgggcggt cttgccctga
    38881 agatgaggga agttgggaat ctatggcgct catcagtgtt tctcctttcg cccgccagag
    38941 taaaaagcaa ccgtttacgt taatgaaact gtctaaacac atgctcgtgg agtgccagtt
    39001 tttgcgagga aggggagtta gagacaggaa ttgcggaggc aatcacataa tcgtggttaa
    39061 accacgaaaa tgagaagaga acctgacaat ggaattatct gtaattttct catattgata
    39121 tttcattaat cattctggca tgtcgcactc tcgtatttaa tcgtttttat ctggttagat
    39181 ctcttttggt cggcaatgtt gaaataatga aagcggcaca gattgagggg caataatttg
    39241 aactcagtcg caaaattata attaaatcgg ccatgctacc taatatatgc ccatttattg
    39301 catcgcgaaa tttgagaaga cataactcat taaatgaaag ggatgcaaat gaataagttt
    39361 atcgctgctg aagctgcgga atgtataggc tgtcatgctt gtgaaattgc ctgtgcgatg
    39421 gcacataatc aagaaaactg gccgctgagt cacagtgact ttcgaccgcg tatccacgtt
    39481 gtagggaaag gccaggctgc gaatccggtg gcctgccatc actgcaacaa tgccccttgc
    39541 gttacggctt gtccggttaa tgctctgact ttccagcccg atagcgtaca actggacgag
    39601 caaaaatgta ttggttgtaa aagatgcgca atcgcttgcc cctttggcgt cgttgagatg
    39661 gtcgatacga ttgcgcagaa atgcgacctt tgtaaccagc gcagttccgg cacgcaagcc
    39721 tgtattgaag tctgcccaac acaggcgtta cggctgatgg acgataaagg gttgcagcag
    39781 ataaaggtgg cccgccagcg caaaacggca gcaggaaaag cgtcatcaga cgctcagcca
    39841 tctcgcagtg cagcgttgct ccccgttaac tcgcgtaaag gcgcagataa aatttcagcg
    39901 agtgaacgga aaacccactt tggcgaaatc tattacgggc tggatccaca acaagcgact
    39961 tatgagagtg accgctgtgt ttattgcgcc gaaaaagcta actgcaactg gcattgcccg
    40021 ctgcataacg ctattccgga ttacatccgt ctggtgcagg aaggaaagat tattgaagcg
    40081 gcagaacttt gccaccagac cagttcctta ccagaaatct gcggcagggt atgtccacag
    40141 gaccgtcttt gtgaaggcgc atgtactttg aaagatcact ctggcgcagt ctctatcggg
    40201 aatctggaac gctatatcac cgataccgcg ctggcgatgg gctggcgtcc cgatgtcagc
    40261 aaagttgttc cccgtatcga aaaagtggcg gtgattggcg ctgggcctgc agggttaggg
    40321 tgtgctgata ttctggcgcg cgcaggagtt caggttgatg tctttgatcg ccatccagaa
    40381 attggcggta tgctgacttt tggcattcct cctttcaaac tcgataaaac ggtattaagc
    40441 cagcggcgag agatattcac cgcaatggga atcgactttc atcttaactg tgaaattggc
    40501 cgcgatatta cctttagcga tttaacttct gaatatgatg cagttttcat cggcgtgggg
    40561 acttacggga tgatgcgagc agatctgccg catgaagatg cgcccggtgt cattcaggct
    40621 ctaccgttcc tgactgccca tacccgccag ctcatggggt tgccggagtc tgaagagtat
    40681 ccgctgacgg atgtggaagg taagcgggtc gtggtattgg gcggtggcga tacgaccatg
    40741 gattgtttgc ggacttccat ccgcctcaat gccgccagcg tgacctgcgc gtatcgtcgt
    40801 gatgaagtca gtatgccggg ctcgcgcaaa gaggtggtca atgcgcgcga ggaaggtgtc
    40861 gagtttcagt tcaatgttca gccgcaatat atcgcttgtg acgaagatgg gcgtttaact
    40921 gcggtgggcc tgattcgtac agccatgggt gagccggggc cggatggtcg ccgtcgtcct
    40981 cgtccggtag cgggttcaga atttgaattg cccgccgatg ttctgattat ggcctttggt
    41041 ttccaggcgc atgccatgcc gtggttgcag ggcagcggaa ttaaactcga taaatggggc
    41101 ctgattcaaa ccggcgacgt cgggtattta cctacccaga cgcacctgaa aaaagtcttt
    41161 gctggtgggg atgcagttca tggcgcggat ctggttgtca ctgcaatggc cgcaggaagg
    41221 caggcggcgc gcgatatgtt aactctgttt gatacgaagg catggtgatg aaatcgttaa
    41281 ttatcgttaa tccggctgac tgtattggct gccgtacttg tgaagtggcc tgtgtggtcg
    41341 ctcacccttc agaacaggag ttgaatgccg atgtcttttt accccggctg aaggtacagc
    41401 gactggactg catcagtgcg ccggtgatgt gccatcagtg tgaaaacgcc ccttgtgttg
    41461 gcgcttgccc cgtgggagcg ctgacgatgg gcgagcaggt ggtgcaggcc aattctgccc
    41521 gttgtattgg ctgtcagagt tgcgtcagtg cgtgcccgtt tgggatgata accattcagt
    41581 cgttgccggg ggatacccgg caacaaattg tgaaatgcga tctctgtgaa cagcgggaag
    41641 aggggcctgc ctgcgttgaa tcttgcccga cacaggcgct acagttgctt actgaaagag
    41701 aactcaggcg agtccgccag cagcgtattg ttgccagcgg tgagaatcct
//
LOCUS       DAPOZO010000044        38650 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPOZO010000044 DAPOZO010000000
VERSION     DAPOZO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.044"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            207..2192
                     /gene="yjcS"
                     /locus_tag="ROI21_002391"
     CDS             207..2192
                     /gene="yjcS"
                     /locus_tag="ROI21_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDX5111303.1"
                     /translation="MNNSRLFRLSRIVIALTAASSMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTLRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPEANASFYISREDLHAVLTGQVKMA
                     DLVKAKKAKIIGNGAILEEIIACLDNFDLWVNIVTPN"
     gene            2401..2676
                     /locus_tag="ROI21_002392"
     CDS             2401..2676
                     /locus_tag="ROI21_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111304.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLLWLAFF"
     gene            2695..3726
                     /gene="mdtN"
                     /locus_tag="ROI21_002393"
     CDS             2695..3726
                     /gene="mdtN"
                     /locus_tag="ROI21_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDX5111305.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAGLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGT
                     SAVANLEPQ"
     gene            3726..5777
                     /gene="mdtO"
                     /locus_tag="ROI21_002394"
     CDS             3726..5777
                     /gene="mdtO"
                     /locus_tag="ROI21_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDX5111306.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQYAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMALRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            5774..7240
                     /gene="mdtP"
                     /locus_tag="ROI21_002395"
     CDS             5774..7240
                     /gene="mdtP"
                     /locus_tag="ROI21_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDX5111307.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRRDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQIQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVLQSIQLMKSLGGGYQASPVVEKK"
     gene            7438..9585
                     /gene="fdhF"
                     /locus_tag="ROI21_002396"
     CDS             7438..9585
                     /gene="fdhF"
                     /locus_tag="ROI21_002396"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7855..7857,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDX5111308.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            9679..10368
                     /locus_tag="ROI21_002397"
     CDS             9679..10368
                     /locus_tag="ROI21_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDX5111309.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(10447..11760)
                     /gene="gltP"
                     /locus_tag="ROI21_002398"
     CDS             complement(10447..11760)
                     /gene="gltP"
                     /locus_tag="ROI21_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDX5111310.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12102..12698)
                     /gene="nrfG"
                     /locus_tag="ROI21_002399"
     CDS             complement(12102..12698)
                     /gene="nrfG"
                     /locus_tag="ROI21_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDX5111311.1"
                     /translation="MKPPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALHL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(12695..13078)
                     /gene="nrfF"
                     /locus_tag="ROI21_002400"
     CDS             complement(12695..13078)
                     /gene="nrfF"
                     /locus_tag="ROI21_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDX5111312.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(13071..14789)
                     /locus_tag="ROI21_002401"
     CDS             complement(13071..14789)
                     /locus_tag="ROI21_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDX5111313.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLMTFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRSEFDGACARICWRWALPGW
                     GALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFAGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQCGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(14809..15765)
                     /gene="nrfD"
                     /locus_tag="ROI21_002402"
     CDS             complement(14809..15765)
                     /gene="nrfD"
                     /locus_tag="ROI21_002402"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDX5111314.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVMLRRVYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAMRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(15762..16433)
                     /gene="nrfC"
                     /locus_tag="ROI21_002403"
     CDS             complement(15762..16433)
                     /gene="nrfC"
                     /locus_tag="ROI21_002403"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDX5111315.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(16430..16996)
                     /gene="nrfB"
                     /locus_tag="ROI21_002404"
     CDS             complement(16430..16996)
                     /gene="nrfB"
                     /locus_tag="ROI21_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDX5111316.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(17041..18477)
                     /gene="nrfA"
                     /locus_tag="ROI21_002405"
     CDS             complement(17041..18477)
                     /gene="nrfA"
                     /locus_tag="ROI21_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDX5111317.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTATPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISIKEKAQKAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            18870..20828
                     /gene="acs"
                     /locus_tag="ROI21_002406"
     CDS             18870..20828
                     /gene="acs"
                     /locus_tag="ROI21_002406"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDX5111318.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLGLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            21054..21368
                     /locus_tag="ROI21_002407"
     CDS             21054..21368
                     /locus_tag="ROI21_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDX5111319.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            21365..23014
                     /gene="actP"
                     /locus_tag="ROI21_002408"
     CDS             21365..23014
                     /gene="actP"
                     /locus_tag="ROI21_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDX5111320.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            23192..24484
                     /gene="espX5"
                     /locus_tag="ROI21_002409"
     CDS             23192..24484
                     /gene="espX5"
                     /locus_tag="ROI21_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HDX5111321.1"
                     /translation="MCYNGLNNMFFPLCQINNNHSVTSPSHTKKTKSDNYRKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRFYDSLPSGKITKETDRKIHKYFIDIASHANNK
                     CDDRITRRVYLNKDKEVSIKVVYFINNVTVHNNTIEIPQTVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFNFAIMEKILFDNCILDDSNFAQIKMTDGT
                     LNACSAMHVQFVNATMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFRADLSF
                     SKLDLINFEHADLSRVNLNKATLQNINLIDSKLFFTRLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSVLTKAWMFNTRLYRVNFDEANVQGMGISILRGEENIPIDSDILVT
                     LQKFFEEDCTSHTGMSQTEDNIHAVAMKITADIMQHAD"
     gene            complement(24638..26287)
                     /locus_tag="ROI21_002410"
     CDS             complement(24638..26287)
                     /locus_tag="ROI21_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDX5111322.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(26438..27787)
                     /gene="ghxP"
                     /locus_tag="ROI21_002411"
                     /gene_synonym="yjcD"
     CDS             complement(26438..27787)
                     /gene="ghxP"
                     /locus_tag="ROI21_002411"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDX5111323.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(28333..28797)
                     /gene="soxR"
                     /locus_tag="ROI21_002412"
     CDS             complement(28333..28797)
                     /gene="soxR"
                     /locus_tag="ROI21_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDX5111324.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            28883..29206
                     /gene="soxS"
                     /locus_tag="ROI21_002413"
     CDS             28883..29206
                     /gene="soxS"
                     /locus_tag="ROI21_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDX5111325.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(29209..30795)
                     /gene="pdeC"
                     /locus_tag="ROI21_002414"
     CDS             complement(29209..30795)
                     /gene="pdeC"
                     /locus_tag="ROI21_002414"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDX5111326.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVVVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIGQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            31225..31506
                     /locus_tag="ROI21_002415"
     CDS             31225..31506
                     /locus_tag="ROI21_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDX5111327.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            31876..32694
                     /locus_tag="ROI21_002416"
     CDS             31876..32694
                     /locus_tag="ROI21_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
                     /protein_id="HDX5111328.1"
                     /translation="MSFSSTHTRWLTKNEEDLRTADGKNIEVWVFRHEQDEQVLSEWA
                     KHFRNHYCFDSEIDYFRRGYGYSREDYLNLIKFPDIKNAPGPSIRAGDFGEVLVADFI
                     EYLLGYWVPRTRYGDKTIRNESTKGSDIIGFHIIKDGIVSSNDRLAIFEAKAQFSGKK
                     AKPRLQDAVDGSLKDVARKAESLNAIKQRLYGRNQLDDAEKIERFQNEVDYPYKEVYG
                     AVALFDSPLFDSHLTSLTDTISHPYSGDLVLVVITGDQMMALVHELYRRAAHEA"
     gene            32684..35839
                     /locus_tag="ROI21_002417"
     CDS             32684..35839
                     /locus_tag="ROI21_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDX5111329.1"
                     /translation="MRPEKKSQLLLGVSRSKAKMLEFSIPEEHHIKITQDPAKLFSIS
                     IGLLGDLAAAINREEPEVNLLTEMKTNLLFSASFFDSYLQSKLNETLDPYLMLLGAAS
                     YYLCELPGSSSVLIRKLKSDIDASGIYGLYCPNLDGAGLEFLLLWLLEGDSSIHFENI
                     DSAFGKPIENISKELNIFFSTGNDFAIRSEVKRLRDLAYFAGTPRQLLLSDLISSITL
                     KKIDNSTWKCLPKYSGLSISTWMEALHKNSFIKEFWPAQHLLGQANVLRGKSAIVQMP
                     TSAGKTKATELILRSAFLAKRVSLAIIIAPFRALCHEIKNSLVEAFNSESTKVDELTD
                     TLQTDFEIAELLGHQQILVVTPEKLLYALRHAPELGAHIGLLILDEGHQFDSGTRGIT
                     YELLLTSLRTMIPEGTQKVLISAVISNAEAVGLWLNGEPNVVEGTTLIPTFRSIGFAS
                     WLDQLGRIEYVDSRNAEQSEFFVPRVIETFNLGRKPREHTDRFFPEKNNGQAIALYLG
                     LKLAPKGSIAIFCGRKSTAASICEKAVDIIKRGVPLPLPSDYSDPQEVARLTHLHIKN
                     LGANAPASQSAEHGIFSHHGNTPQGIRLAVEHAMRDNLVRFVVCTSTLAQGVNLPIRY
                     LIVTSVYQGMERIKVRDFHNLIGRAGRAGMHTEGSILFADPVIFDKRKVLSEKWRWKQ
                     VKKLLEPQNSEPCVSNLLSIFDPIKSDDEKISIPMGALDFTKAYIDNPEKIANLATEI
                     TALHGDKNFSQNNVERQIAWRISLISAVESFLLSHWDESEDGLSQADVTHLAEGTLAF
                     FLADTQKKEHIRELFQLLARHISTNITEPSRRKIYGRTLYGIQDAQSIEKWVQSNTDS
                     LLSIVDEKEILNHIWQLLIQHINSGVFTKFDEPEVLKEISYEWISGKPFNDLLEIIHE
                     RKVKMIWGTNRREFKIDHIVDICEGALAYNATLVVGAISEFIETLNKDGTRDLFNRLQ
                     LLQKKLKYGLPTDTAISLYELGFSDRVIAQDLATSLKLAATQKKYLVKELKNNRGNAM
                     KVIEKYPMYFQERMSELIEDF"
     gene            complement(36567..38609)
                     /locus_tag="ROI21_002418"
     CDS             complement(36567..38609)
                     /locus_tag="ROI21_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HDX5111330.1"
                     /translation="MSKKALSINFPIERVVLSDFLPYEVPIIFSNRHFYKFIVKHQIK
                     HDGLNFIWLKRDGCLNSLILLLLGLEQNTETVEIVDKGISYTKTNIKYAKLPSIAFTF
                     PISHKHKEYRYLSLMHPKGQLLGVDFYRNYKDLITYYSNVSPFSLRSAKRIAGCTYID
                     SKSLIESFEKEDLSIEVEGQDYENLKSFFVYKKYRNIFEFYESEDHHWCEKRFNFLSK
                     LDVSNCFDSIYTHSISWAVLQKNYSKQQLNKTQSTFPFKFDQLMQRSNYNETNGIIIG
                     PELSRIFAEIIMQSVDNNIINDLNKDGLVLGGDYQVYRYVDDYFIFYNDDDVFNKIRI
                     STQKNLKTFKLSLNKYKEERFSRPIITPITIAKKKIANLISERISYNITDNGSLEGDI
                     KKGNVYIHQNSLSTDFKSILSVSGITYSDILNYSLSIIERKIKTLFKDYKEFSKDESG
                     ERQLINAIISVVNFTYFIYAVAPKVNSTIKLCRIIQQVIMFVKDQKICEEYQHIVFKC
                     VFDNSCSILDKYSVDKTTPIETLYLLVLIRQLGKYYWLGEKSLMKYFNISFEGGAYSF
                     DDNLNYFSITSLMFYMSGKKRYDGVRKHLLLHIENIYSMKVDTLLNESELVHLTLDLI
                     ACPYISVSFKKALLSRYGIEKSYVKRIISLNEYWFTKWNDFDFAKELDAKLSNAVY"
BASE COUNT        10235 a         9781 c         9331 g         9303 t
ORIGIN      
        1 atatataaaa cacctcatat tatttacaga gataataaat tcgcgtactt ttccctcttc
       61 agcacaaatg cctaataaaa tggcgcgttc agctcacact tctacttatt aaagtcgcaa
      121 tttcccaatt atgataacgc cagcttcaaa tgacgtattt tgcatgggtt acatatttac
      181 gacataaatg ataaatggag atttatatga ataactctcg gttattccgt ttgagcagga
      241 ttgttattgc gttaactgcc gccagcagca tgatggtaaa taccgctaat gcgaaagagg
      301 aagcgaaagc cgccactcaa tatacccaac aggttaatca gaattacgcc aaatcattac
      361 cgtttagcga tcgtcaagat tttgacgatg cccagcgtgg atttatcgcc ccgctgctgg
      421 atgaaggtat tctgcgtgat gcgaacggta aagtttacta ccgcgcagac gattacaaat
      481 ttgatattaa tgccgctgcg ccggaaaccg taaaccccag cctgtggcgt cagtcgcaaa
      541 tcaacggtat ttctggcctg ttcaaagtca ccgataaaat gtatcaggtg cgcggccagg
      601 atatctctaa cattacgttc gttgagggcg agaaaggcat tattgttatc gacccgctgg
      661 tgacgccgcc tgccgcaaaa gccgcacttg atctttactt ccagcatcgt ccgcaaaaac
      721 cgattgttgc tgtgatttac acccacagcc acgccgacca ttatggcggt gtgaaaggca
      781 ttatctctga agcggatgtt aaatccggca aagtgcaggt gatcgctccg gcaggcttta
      841 tggacgaagc catcagcgaa aacgtgctgg cgggtaacat catgagccgc cgtgcgctct
      901 actcttacgg tctgttactg ccgcacaacg cgcaaggcaa cgtgggcaac ggcctgggcg
      961 tgacgctggc aacgggcgac ccgagcatta ttgcaccgac taaaactatc gtcagaactg
     1021 gcgagaagat gattatcgac ggcctggaat ttgacttcct gatgactccg ggtagcgaag
     1081 cgccagccga aatgcacttc tatattccgg ctctgaaagc cctgtgtacc gccgagaacg
     1141 ccacgcatac cctgcacaac ttctacactc tgcgcggcgc gaaaacccgc gacaccagca
     1201 agtggaccga gtatctgaac gaaacgctgg atatgtgggg taatgacgcg gaagtactgt
     1261 ttatgccgca cacctggccg gtctggggca ataagcatat caatgattat attggtaaat
     1321 atcgcgatac tatcaagtac attcacgacc agaccctgca cctggctaac cagggctaca
     1381 ccatgaatga aatcggcgac atgattaagc tgccgcctgc gcttgccaat aactgggcca
     1441 gccgcggcta ttacggttct gtcagccaca acgcccgcgc ggtgtataac ttctatcttg
     1501 gctattacga cggtaacccg gctaacctgc atccgtatgg tcaggtggag atgggtaaac
     1561 gttacgtgaa agcgctgggc ggttctgccc gcgtcatcaa cctggcgcaa gaagcgaaca
     1621 agcaaggtga ttaccgctgg tcggcagaac tgctgaaaca ggtgattgcc gccaaccctg
     1681 gtgaccaggt cgcgaagaat ctgcaagcga ataactttga acagctgggc tatcaggccg
     1741 agtccgccac ctggcgcggt ttctacctga ccggcgcgaa agagctgcgc gaaggggtgc
     1801 ataaattcag ccacggcacc accggttccc cggacaccct tcgcggaatg tcggtcgaaa
     1861 tgctgttcga ctttatgtcc gttcgcctcg atagtgcgaa agccgcgggt aaaaatatca
     1921 gcctgaactt caatatgagc aacggcgata acctcaacct gacgctgaac gatagcgtgc
     1981 ttaactaccg taaaacgctg caacctgaag ccaatgcctc tttctacatc agccgtgaag
     2041 atcttcacgc cgtgctgacc ggacaagtca aaatggcgga tctggtaaaa gcgaagaaag
     2101 ccaaaattat tggcaatggc gcgatactgg aagaaattat tgcctgtctg gataatttcg
     2161 atttgtgggt gaatatcgta accccaaatt aatgccactc acttttgggg gcgcttaagc
     2221 ccccaaaagc atttatttta aaaatacata caacaaatta atacataaaa tatataagtt
     2281 atgaatgcgg tggctgaata aagctatttt tattcatcga gaaaatagcc atactgacgc
     2341 cttcaatgga tattgatatt cactacagat attgttcgca gcagacagga tgtatttaat
     2401 atgccaacag ttctctctcg catggcgatg caactcaaaa aaacagcctg gataattccc
     2461 gtcttcatgg tttcgggatg ctcattatct ccggcaatcc cggtgatcgg cgcttattat
     2521 cccagctggt ttttctgcgc cattgccagc cttattttga cgctcatcac gaggcgaatt
     2581 attcagcggg caaatatcaa tctggcattt gtcggaatta tttataccgc cctttttgct
     2641 ctctacgcca tgctgctctg gctggcattt ttctaatcta tttgagacgc gctcatggaa
     2701 agtacgccga aaaaagctcc tcgcagtaaa ttccctgctc tgttagtggt tgcgttggcg
     2761 ctggttgccc ttgttttcgt tatctggcgc gtagacagtg cgccatcaac taatgacgcc
     2821 tacgcgtcag cagataccat tgatgtggtg ccggaagtca gcggtcgcat tgtagaactg
     2881 gcggtcaccg acaaccaggc ggtgaaacag ggcgatttgc tgtttcgcat cgacccgcgc
     2941 ccatacgagg ccaatctggc gaaagccgaa gcctccctcg cggcgctgga taagcaaatt
     3001 atgctcaccc agcgtagcgt tgacgcgcaa cagtttggtg ccgactcggt taatgccacg
     3061 gtagaaaaag cccgtgccgc cgcgaaacag gccagtgata cattacgccg caccgagcca
     3121 ttactgaaag aaggtttcgt ctcagcggaa gacgttgacc gcgcgagaac cgcgcagcgc
     3181 gccgcagaag ccgatcttaa tgccggattg ttgcaggcgc agtcagccgc cagcgccgtc
     3241 agcggcgtgg atgcgctggt ggcgcaacgt gcggcagtgg aagcggatat tgcgttgacg
     3301 aaactgcatc tggaaatggc gaccgttcgc gcgccgtttg atggccgggt catttccctc
     3361 aaaacctccg tcgggcaatt tgcttctgcc atgcgcccta ttttcaccct aatcgacact
     3421 cgtcactggt acgtgatcgc caacttccgc gaaactgatc tgaaaaatat tcgctcaggt
     3481 acacccgcaa ccattcgcct gatgagcgac agcggcaaaa ccttcgaggg taaagtggat
     3541 tcgattggct acggcgtgct accggatgac ggcggcctgg tgctgggcgg cctgccgaaa
     3601 gtgtctcgtt ctattaactg ggtccgcgtt gcccagcgtt ttccggtcaa aatcatggtt
     3661 gataaacctg acccggaaat gttccgcatc ggcacttcgg cagtcgctaa tcttgagccg
     3721 caataatgag cgcgctcaac tccctgccat taccggtggt caggctgctg gcgttctttc
     3781 atgaagagtt aagcgagcgg cggccaggtc gtgtgccgca gaccgtgcaa ctctgggtag
     3841 gctgcctact ggtgattctg atctcgatga cttttgagat cccttttgtg gcgttatcgc
     3901 tggcagtgct gttttacggt attcagtcga acgcgtttta caccaaattt gtggcgatct
     3961 tgtttgtggt tgccacggtg ctggagatcg gcagcctgtt tttgatctac aaatggtcat
     4021 acggcgaacc gttgatccga ttgatcatcg ccgggccgat cctgatgggc tgcatgtttt
     4081 tgatgcgcac ccatcggctg ggactggtct ttttcgctgt cgccattgtc gccatttacg
     4141 ggcaaacctt ccccgccatg ctcgactatc cggaagtggt cgtgcgctta acgctgtggt
     4201 gtatcgttgt tggcctctat ccaaccttgc tgatgacgtt aatcggcgtg ctgtggtttc
     4261 ccagtcgtgc cattacgcaa atgcatcaag cgcttaatga tcggcttgat gatgccatta
     4321 gtcacctgac ggacagcctc gcaccgctac ccgaaacgcg gattgaaaga gaggcgctgg
     4381 cgctgcaaaa actcaatgtc ttttgcctcg cggacgatgc caactggcga actcagagcg
     4441 catggtggca aagctgcgtg gcaacggtaa cctacattta ctcgacgctg aatcgctacg
     4501 atcccacctc ttttgctgat tctcaggcaa ttattgaatt tcggcaaaaa ttagcctcag
     4561 aaatcaacaa actgcagtat gccattgccg aaggtcagtg ctggcaaagc gactggcgga
     4621 tcactgaaag tgaagcgatg gcggcacggg aatgtaacct ggagaatatc tgccagacgt
     4681 tattgcaact gggtcagatg gacccgaata cgccgccaac gcccgccgcc aaaccgccat
     4741 caatggtcgc cgacgctttt accaatccag actatattcg ctacgcggta aaaacgctgc
     4801 tcgcctgttt gatctgttac accttctaca gcggcgtgga ctgggaaggc attcacacct
     4861 gtatgctgac ctgcgtgatc gtcgctaacc cgaatgttgg ttcgtcgtat cagaaaatgg
     4921 cgctgcgttt tggcggtgcc ttctgcggtg caattctggc gctgttattc acgttactgg
     4981 tcatgccctg gctggacaat attgtcgaat tgctgtttgt gctggcaccg attttcctgt
     5041 tgggcgcatg gattgccacc agctctgaac gctcttctta tatcggcaca cagatggtgg
     5101 tcaccttcgc gctcgccacg ctcgaaaacg tttttggccc agtgtacgac ctggtggaaa
     5161 ttcgcgatcg cgccctgggt atcatcattg gtaccgtggt gtccgcggtg atttacacct
     5221 ttgtctggcc tgaaagtgaa gcgcgcacac tgccgcaaaa actggctggc gcgctgggta
     5281 tgttgagtaa agtaatgcgg atcccacgcc agcaggaagt cacggctctg cgcacttatc
     5341 tgcaaattcg tataggtctg catgcggcgt ttaatgcctg tgaagagatg tgccaacgcg
     5401 tggcgctgga gcgtcaactg gacagcgaag aacgcgcttt actgattgaa cgttcgcaaa
     5461 cggttattcg tcagggccgc gatattcttc acgcctggga tgcaacctgg aactcggcgc
     5521 aggcgctgga taacgcacta cagccggaca gagcaggtca gtttgccgac gcactggaga
     5581 aatacgctgc cggtctggca accgcactca gccgttctcc tcaaataacg cttgaagaaa
     5641 cacccgcctc gcaggccatc ctgccgacct tattaaaaca ggagcaacac gtctgccagc
     5701 ttttcgcccg cttgccagac tggacagccc cggcattaac gcccgccacg gaacaggtac
     5761 aaggagccac gcaatgatca atcgtcaact ttcacgcctg ctgttgtgca gcattctcgg
     5821 cagcacgacg ctgatttccg gctgtgccct ggtacgcagg gattctgcgc ctcatcaaca
     5881 gctcaaacct gaacaaatca aactggccga tgatattcat cttgccagct ccggctggcc
     5941 gcaggcgcag tggtggaaac aactcaatga cccgcagttg gatgccctga tccaacggac
     6001 gctaagtggt tcacacaccc tcgccgaagc gaaactgcgg gaagaaaaag cgcagtccca
     6061 ggccgatttg ttagatgctg gttcacagtt gcaggtggca gcgttaggga tgctcaaccg
     6121 ccaacgtgtc tcggcgaacg gctttttaag cccttatgcg atggatgcgc ccgcactggg
     6181 gatggacggg ccgtactata cggaagccac agtcggtttg tttgccggat tggatctcga
     6241 tttgtggggt gtgcatcgct cagcggttgc cgctgccatt ggcgcgcata atgccgcgct
     6301 ggcagaaacc gcagcagtag agctatcgct gaccacgggc gtagcgcagc tttattacag
     6361 tatgcaggcc agctatcaga tgctcgatct gttagaacaa actcgcgatg tgattgatta
     6421 cgcggtaaaa gcgcaccaga gtaaagtggc gcacggtctg gaagcgcaag tgcctttcca
     6481 cggcgcgcgg gcacagattc tggcggtcga taaacaaatt gctgccgtta aagggcaaat
     6541 caccgaaacg cgagaatctc tgcgcgcatt gattggcgcg ggagccagcg atatgccgga
     6601 gatcaaaccg gtggcattac cgcaaatcca gaccggtatt ccggcgacac tctcttatga
     6661 gttgctcgcc agacgcccgg atctgcaagc tatgcgctgg tatgttcagg cgtcattaga
     6721 tcaggtggat tccgcgcggg cgctgttcta tccgagcttt gatatcaaag cgtttttcgg
     6781 cctggattct atccacctgg ataccttatt caaaaaaacc agtcgccagt tcaactttat
     6841 cccaggtctg aaattgccgc tgtttgacgg tggacggttg aatgccaatc tcgaaggcac
     6901 tcgcgccgcc agcaacatga tgattgaacg ttacaaccag tcagtgctga atgcggtgcg
     6961 cgacgttgcc gtcaacggca cgcgtctgca aacgctcaac gacgagcgag agatgcaggc
     7021 tgaacgcgtg gaagcaacgc gcttcaccca gcgcgctgcc gaggccgcct atcaacgcgg
     7081 cttaaccagc cgcttacagg ccaccgaagc ccggttgcca gtgcttgccg aggagatgtc
     7141 attactgatg ctggacagcc gccgggtgct ccaaagcatt cagttgatga aatcgctggg
     7201 cggcgggtat caggcaagtc ccgtcgtcga gaaaaaataa aatgtctgcc gcgtgatggc
     7261 tgtcacgcgg tatttcgttt cgtcacgtca aaactgacga cagcctgttt ttcgtcagag
     7321 ttttgaataa atagtgcccg taatatcagg gaatgacccc aaataaaatg tggcataaaa
     7381 gatgcatact gtagtcgaga gcgcgtatgc gtgatttgat taactggagc gagaccgatg
     7441 aaaaaagtcg tcacggtttg cccctattgc gcatcaggtt gcaaaatcaa cctggtcgtc
     7501 gataacggca aaatcgtccg ggcggaggca gcgcagggga aaaccaacca gggtaccctg
     7561 tgtctgaagg gttattatgg ctgggacttc attaacgata cccagatcct gaccccgcgc
     7621 ctgaaaaccc ccatgatccg tcgccagcgt ggcggcaaac tcgaacctgt ttcctgggat
     7681 gaggccctga attacgttgc cgagcgcctg agcgccatca aagagaagta cggtccggat
     7741 gccatccaga cgaccggctc ctcgcgtggt acgggtaacg aaaccaacta tgtaatgcaa
     7801 aaatttgcgc gcgccgttat tggtaccaat aacgttgact gctgcgctcg tgtctgacac
     7861 ggcccatcgg ttgcaggtct gcaccaatcg gtcggtaatg gcgcaatgag caatgctatt
     7921 aacgaaattg ataataccga tttagtgttc gttttcgggt acaacccggc ggattcccac
     7981 ccaatcgtgg cgaatcacgt aattaacgct aaacgtaacg gggcgaaaat tatcgtctgc
     8041 gatccgcgca aaattgaaac cgcgcgcatt gcggacatgc acattgcact gaaaaacggc
     8101 tcgaacatcg cgctgttgaa tgcgatgggc catgtcatta ttgaagaaaa tctgtacgac
     8161 aaagcgttcg tcgcttcccg cacagaaggc tttgaagagt atcgtaaaat cgttgaaggc
     8221 tacacgccgg agtcggttga agatatcacc ggcgtcagcg ccagtgagat tcgtcaggcg
     8281 gcgcggatgt atgcccaggc gaaaagcgcc gccatcctgt ggggcatggg tgtaacccag
     8341 ttctaccagg gcgtggaaac cgtgcgttct ctgaccagcc tcgcgatgct gactggtaac
     8401 ctcggtaagc cgcatgcggg tgtaaacccg gttcgtggtc agaacaacgt gcagggtgcc
     8461 tgcgatatgg gcgcgctgcc ggatacgtat ccgggatacc agtacgtgaa agatccagct
     8521 aaccgtgaga aattcgccaa agcctggggc gtggaaagcc tgccagcgca taccggctat
     8581 cgcatcagcg agctgccgca ccgcgtagcg catggcgaag tgcgtgccgc gtacattatg
     8641 ggcgaagatc cgctgcaaac tgacgcggag ctgtcggcag tacgtaaagc ctttgaagat
     8701 ctggaactgg ttatcgttca ggacatcttt atgaccaaaa ccgcgtcggc ggcggatgtt
     8761 attttaccgt caacgtcgtg gggcgagcat gaaggcgtgt ttactgcggc tgaccgtggc
     8821 ttccagcgtt tcttcaaagc agttgagccg aaatgggatc tgaaaacgga ctggcaaatc
     8881 atcagtgaaa ttgccactcg tatgggttat ccgatgcact acaacaacac ccaggagatc
     8941 tgggatgagt tgcgtcatct gtgcccggat ttctacggtg cgacttacga gaaaatgggc
     9001 gaactgggct tcattcagtg gccttgccgc gatacttcag atgccgatca ggggacttct
     9061 tatctgttta aagagaagtt tgataccccg aacggtctgg cgcagttctt cacctgcgac
     9121 tgggtagcgc caatcgacaa actcaccgac gagtatccga tggtactgtc aacggtgcgt
     9181 gaagttggtc actactcttg ccgttcgatg accggtaact gtgcggcact ggcggcgctg
     9241 gctgatgaac ctggctacgc acaaatcaat accgaagacg ccaaacgtct gggtattgaa
     9301 gatgaggcat tggtttgggt gcactcgcgt aaaggcaaaa ttatcacccg tgcgcaggtc
     9361 agcgatcgtc cgaacaaagg ggcgatttat atgacctacc agtggtggat tggtgcctgt
     9421 aacgagctgg ttaccgaaaa cttaagcccg attacgaaaa cgccggaata caaatactgc
     9481 gccgtgcgcg tcgagccgat cgccgatcag cgcgccgccg agcagtacgt gattgacgag
     9541 tacaacaagt tgaaaactcg cctgcgcgaa gcggcactgg cgtaatacag tcctttctac
     9601 agcctccttt cggaggctgt ttttttatcc attcgaactc tttatactgg ttactcccta
     9661 cccaatcgta ttatcaaaat gaaaaaaatt atcgcattga tgttgttttt gacattcttt
     9721 gcccacgcga acgactccga gcctggcagc cagtatttaa aggcagcaga ggccggggac
     9781 cgacgcgcac aatattttct tgccgacagc tggtttagct ccggcgattt gagcaaagcc
     9841 gaatattggg cacagaaagc cgccgacagc ggtgatgctg atgcctgcgc gctgttggcg
     9901 cagatcaaaa tcaccaatcc ggtcagtctg gactatccac aagcaaaagt tcttgcagag
     9961 aaagcagcgc aagcgggcag taaagaaggt gaagtaacgc tggcgcatat tctggtaaat
    10021 acgcaagcgg gtaaaccgga ttatccaaag gcgatttcgc tgttagaaaa cgcttcggaa
    10081 gatctggaga acgactctgc cgtcgatgcc caaatgctgc ttggtttgat ttacgccaac
    10141 ggcgtgggca ttaaggccga cgatgacaag gcaacctggt atttcaaacg cagctctgcg
    10201 atttcccgaa ccggttattc cgagtactgg gcgggaatga tgttcttaaa tggtgaagaa
    10261 ggttttatcg agaagaataa acaaaaggcg ctgcactggt tgaacctgag ctgtatggaa
    10321 gggtttgata ccgggtgtga agagtttgag aaattaacca acggttaagg taggcctgat
    10381 aagacgcgtt aagcgtcgca tcaggcatcc acacattgcc gggtggatat ccccggcaat
    10441 cttcaattat tgatccgcag ttttatcaaa tttgcccagc acttcacgct cataagccag
    10501 cgctttctta cggtcaaatt tgtgttccca cttggcaatc accagcaccg ccagcgcatt
    10561 acccaccacg ttcagcgcag tacgcgccat atcgaggata cggtcaacac cggcaataaa
    10621 cgccagacct tccagcggaa tacctacgct acccagcgtt gccagcagca ccacaaacga
    10681 cacgcccggc acgccagcaa tccctttcga ggtcaccatc agcgtcagca ccagaatgat
    10741 ttcctgccag atggacaggt caatgccata cagctgagcg atgaaaatag cggcaatact
    10801 ttgatacagc gtcgaaccat caaggttaaa agagtaaccg gtcggcacca cgaaactggt
    10861 gatcgacgcc ggtgctccgt aggcttccat cttctcaata attcgcggca gcacgctttc
    10921 agagcttgca gtggagtacg ccagaatcag ctcatctttc agaatacgaa tcaggatcca
    10981 gacgcttaac ccgcacaggc gcgccacaat tcccagcact accagcgcga agaacagaat
    11041 ggcgaaatgc accagcagca ccagtttcgc cagtggccac agagacgaga aaccaaagtt
    11101 agccaccgtc accgcaatca gcgcaaacac acccaccggc gcataacgca tcaccatgtg
    11161 agtcacttta aacatggttt cagagatgga gcggaacacg gtcaccagcg gttcacgatg
    11221 cgtcgcgggc agggaggaaa gccccagacc aaacagcacc gagaagaaga tgatcggcag
    11281 catttcgcct ttcgccatcg acgccacaat gttcgtcggc accagcgaca aaatcgtgcc
    11341 cataatgccg tgggaactgc tttgtaccgc ttccgtagtg ctctgatatt tcgagatatc
    11401 gacggtcgcc agctgcgaca tatccacccc ggcaccgggc tggaagacgt tcgccagagt
    11461 gatccccaaa atgatggcga cggtggtgat cacctcgaag taaataatgg ttttcgcgcc
    11521 aatacgcccg agctgtttgg catcaccaac acccgcgata cccaccacca gcgtggagat
    11581 cacaatcggc acaacaatca ttttgatcag atggatgaag atatcacccg ccggagagag
    11641 caaattgacg accagccagt cgcggctgtc gctatggtag tgcaggtagc ttcccaggag
    11701 aatgcccagc accatagcaa acagaatctg ccaggccagg ctgaatttta tatttttcat
    11761 cagaataact tcctcaatgg agcgccgttc cccgttcaga aacgcgagaa cgtgacatac
    11821 tgccagggca tgaattgtgt tgcgttggtt tgtagatttt tttaacgctt atgatcagta
    11881 tcatttgcgc ggtatgttgc gcaacccttt aacaacagag cttttcgctg cattaatgca
    11941 taataacgcg agtttatggc aggattaatg aataaccatt tgttatggaa agatttttat
    12001 ttgtttaatc tgcaataata ctgattggtg attatttttg gcatatgttc attcgattat
    12061 tttttatgca gctcaataac ataatcttca tcaccagatg attaatccga tctccgctgc
    12121 aacagtttcg ccatattaat cgactcgacc agctgtgtcc ggttaatccg gggtgagttg
    12181 agatccatca ctttttgcca tagttcgatg gcttgcgcgt aattcgcctg cataaacgca
    12241 tcggaagcca gcagcatcag agcggtgatt tcgttagagt ccagcgcgag ggctttgtcg
    12301 atcattgcgc gtgtctgggc ggtcatatgc tggctggcct ggtaataaag caccgtcgcc
    12361 agcgccgcat acagttcagc gttctcacca cgtagatgca acgcctgacg gtacgccagc
    12421 agcgaattgc tgtaatcgtt ttgccacaga tagtactcgc ccagtaacgc ccactgttcg
    12481 ctgttttgtg gattagcgcg gattttatcc tgcaatgcct gaagctgcgc ttccggagtt
    12541 tgctggctgg caaactgatg tagcggatcg cgctgacgct gatactccgc acgcaccgcc
    12601 tgccattttg gacttaacag ataactgccg acacagagaa ataccatcaa tatcgtgagt
    12661 gttgtcagca ttttcaccgg tatttgaggc ggtttcatcg cttcgccctt acccgccaga
    12721 ggatcagtgc catcagcagt aacaacacca ctggcagcgc ccacaacacc agagtctgac
    12781 ccgtcaacgg tgggttataa cgaacaaaat caccgtagcg ttcggtcatc cagccaatga
    12841 tttcgacttc acttttcccc tccgccacca tgctgtaaac ctgatggcgc atactgactg
    12901 ccaccggcgc attggattcc agtaagttct gattctggca ctgcgggcag cgtaactggc
    12961 tggcaatatt taacgcctgt tcctgttgtt gcggattggc gaattgccag gtgtctacaa
    13021 cctgagcgcg cgcaaaacag gtaaataaca gcagtagcgt gagaagccct ttattcatcg
    13081 cgcttcctcc cccgccatcc gcttaacaat gcgcccgcaa tcatcaacaa ccctcccccc
    13141 cagatccagc gcacaccgca ttgtacatac aaacgaaaag cgtaacgatc cggcccagtt
    13201 ttctccccca tgaccgcata ccagtcatgg atgccgttcc agcgaattga cggttccatc
    13261 atttgctgac ggcgtgcttc gtaaaaacgc cgctccggcg tcagttcacc aatacgttgc
    13321 tgatggtcaa acagtgccac tatcgctttt tcgctggtgt aattgcctct ggcttgcaga
    13381 tcgaggcgct caaaacggaa ggtgtatcct gccagcgtca cctgctgacc cggctgtaaa
    13441 ttcaggctga tctcctgacg gctgacgctg gagaccacga tccctgcggc aaataacagc
    13501 acgcccgcat gcgccagcag cgccggaagc tgcacgcgtt tgccagagac aaacgtcgcc
    13561 agcacaatca ccaccagcat caacagacca aacggtaacg tcgcgcggtt aaaatacggc
    13621 gcgccaacgg agaggcgtcc ccagcccagc aggccgtaaa tcatcggata aagcgttccc
    13681 accagcacga tcagtagcac cgcgcaaaac agcaacagcg tagcgaggat taacatttct
    13741 cgcgataacc ccgcaaaacg caccgccggg ccaccgtccc gcgctcgcca gccatacaga
    13801 gccagagacg caaggctaat cagtgcaaac aggctgaaca acggcacggc gcggacgtta
    13861 tccagcgcga acgcatgaac cgaaaccaga atgccagaac ggacaattaa ggtacccagc
    13921 agcgacagca tcagagtaac tatcgccagt aacagcgacc agtggcggaa aatcccccgc
    13981 tggcgtgtca gggacaaact gtgcagcagt gcagtggcag aaagccaggg taataaagag
    14041 gcgttttcca ccgggtccca gaaccaccag ccgccccagc ccagttcgca ataggcccac
    14101 caggaaccga ggatgatccc cgccgttaat gccccccagc ctggtagtgc ccagcgccag
    14161 caaattcggg cgcaggcacc atcaaactcg ctgcgcagta aactcgccag cgccacgctc
    14221 gccgccacca tcaaaccgcc atacccgaga taaagcagcg gcggatggaa gataagaccg
    14281 ggatgttgca gcatcggatt gagatcgcgc ccttcgattg ctggtggaaa tatccgcacg
    14341 aagggatcgg accacagcac tacaaacagt agcagtgcgg cgagcatgag agataaaacg
    14401 gctagcgtca gcggaaatag cggatcggtt tgctgccgat aatgccaggc aaacagcgcg
    14461 ctccaggcgg aaagcagcag cacccaaagc agcagcgaac cttcatgacc gccccacacc
    14521 gccgccagtt tgagttccca cgacagcagg ctgtagctat gttgggcgac ataaatgacc
    14581 gagaaatcgc tgatgagaaa acaatacgtc agtacgccaa aggcgagcag caggagcgcg
    14641 aactgcgcca gaatgccgat gcaagtgagt cgcatcatgg caggccagcg taaccgcact
    14701 cccgcgaagg tcatcaacgg ggtcaacacg ttgaccccaa gacttaacaa caacgccaga
    14761 aaaccgactt caggaaggaa tacgtccaga aaccacctct gttctggctt acgccaccgt
    14821 taactgcccg gcgtagagaa tgaaaaaacg cagcatcaac acgccgacca ggctggcccc
    14881 acacgccgcc agcacagcag gaatgccgga actgcgattg acccacggtt tgagcaacat
    14941 tggcacaatc agccccagcc cggcgacacc aagccagaac caccacgtcc agaaaccgcc
    15001 gcccaatgcc gccaccagcg cacgcacttt accgtcatcg cccagggcca gtccgacaaa
    15061 aaacgccacc agcaggaaga tttcgcccca taccacaggg atctccatac ggtgtacaaa
    15121 gtgcgcttcc gtggaatgcg ggttactgcg ttggcgcatc gccatggcga tcagcgccac
    15181 cgccgcaccg gacgagatgc cggagaagag gaacaacacc ggcaggatcg ggttattgag
    15241 gaacggatac gatttcagcg ccgatagcag aaagccggta taagccccca gcagcaccgc
    15301 cagcaccagc atcaatgttt ccagtccgcg atgaacgggc gtcagcaggc tcagaacctt
    15361 ttgcacgatc cccagcttcg gcaaccagcg ttgttgcagg gcaagcaaat catgttcaaa
    15421 gattttcgcc agccacagta ccagcaccac catgtaaagc tgaaacagca tcacccccat
    15481 cgacatcacc gaggtaaaac tgtagtggaa catcagcttc cagaaggtcc acggtcttgt
    15541 caggtggaag acgaggatca acagaccgag gatcaccgcg cccggcccga caatcagcgt
    15601 ggtgcgcagc aacgtgctgt cggcaccgcc cgcctgcggg tagacgcgac gtaacatcac
    15661 ggccagcgtc accagaccag cagaaatacc aatcaaaaac aggtagatgg caatcggcca
    15721 gtcccacacc agcgattcaa aatgaaatgc ggaagtctgc gtcattggct cacctccccg
    15781 tatttaaacg gtacgcggta cagcttcggt ttggttccca gcgccagctt gtagcggtaa
    15841 gtgggcttct ggcgcagcag ttgcgaaatc tcactgttgg gatcgtccag attgccaaac
    15901 gtcagcgcct tcgtcgggca agcctcaacg cacgcgggca gcttaccggc ctgcaaattg
    15961 gtcttacggc agaaatcgca tttgtccgct gtcttcgtga ccggatggat aaagcgcacg
    16021 cggtacggac aggcggcgat gcagtactga caaccgacgc agagatccgg attaacatcg
    16081 acaatcccgc tggcagcatc gcgaaaagac gcaccggtcg ggcagacgtc aacgcacggc
    16141 gcatgatcgc agtgctggca agacttacgg aaaaagcgat atttcacatc aggaaattcg
    16201 ccctgcggct cgctacgaat aatcgtcagg cgcgagacac cttccggcac cttgttcact
    16261 tcccgacaag catccataca ggcggtacag ccgatgcata acgactcgtc atgcaccatg
    16321 ccgtaacgca ccccattgat attcaacgtt ttcgccacca cgcgccctgc ggtgccgctg
    16381 acggctgcca gcacgccgac gccggtgaga aactggcgac gtgaccaggt catggctgct
    16441 ccttaagcaa cggaacagac gccgggttaa agttcggatt ggtgcgctga tcgctgtggc
    16501 aatcgacgca aatcttaatc cgtcctttgt cacttaacgt ctgcatcgta tcttgctgtg
    16561 gatgcaggga atggcagctg gcgcacgcca ctttggttac gtggacatcg tgtggccaga
    16621 acgctttttg caactgttcc ggcagatggc aggacataca gacgctgttc tgctctccaa
    16681 ccttgtacat cggctcgtta aagcgcatca catctttcac cccttcgcgg tgttgcggtg
    16741 atggctggcc gtgacagttg gtgcaggtga ccggcagttt gttattcggg ttgataacgg
    16801 aagcatgttt gccatgcata ccttcggtat ctggcttatg acagtccaga caggcggcat
    16861 ccgggttacg ctgctgggta acttcgtagc gatcaccaga tgcctgcgcc gcaggggtag
    16921 cggtaattcc gttcaggctc cacaacaggc ccgacgccag caccccggca gttaataacg
    16981 aacgtaatac gctcatattc actccattgt gttttccgcc ccttgcgagg cggaacgggg
    17041 ttattggctt aacagaccgt ttttacgtgc ctgctcttcc cactgcggga tcaccgtttt
    17101 gatgaagtcc tgcttctcgg ccttgatttg ttccatgttc aggccaatgg ccttctgggc
    17161 tttctctttg atcgagatat ccgggatctg gatttcatgg gtgatgcctt tggttgccag
    17221 cagtcgcgcc agtttagtgc gcgcatccgc cgctttatcc atcgccgtac cgagcatcct
    17281 cagaccttct tccggtgcgt gcatatgaat gccgtgggaa gcgatcgcca gatcccaacg
    17341 ccactgggca tgacggatat cgtcctgaat cggcttcatt tcggcttccg tcgcgcctgc
    17401 atccagcgcc gctttcgctt cgaagtgagc gtgaaccagt tgatcttcaa ccttgatttt
    17461 caggtcgtta atcgactgct tacgttccgc gaccactttt tgcaaggcag ctttgtcctg
    17521 ggtatggcag ttcgcacaag tctgggcgaa gttatcaaac ggattaccaa ttttatggtc
    17581 ggtgtagagt ttgccttcgg cgttctgcac ttttggcata tggcaatcga tacaggtcac
    17641 gttgtttttg ccatgaatgc ccgctgtcca ggtttcatat tccgggtgct gcgctttcag
    17701 cattggcgtt ttcgacaggg agttagtcca gtcagagaag gcaattttgt cgtaatactg
    17761 ctccatattt tcgactttca tgccgtcatc ccacgggaat ttaaccgctt tgtttttgcc
    17821 gtcgaagtaa tactccacat ggcactgacc gcaaaccatc gattgctggt cgaaacgtcc
    17881 ggctttctca aaaggtttac caatggcttc catcgcgcga gccgcatacg gacgggaaag
    17941 ggttaactcc ggtttgcctt tggcgaactc tggtgaggca gtgttatggc aatcggcaca
    18001 acctaagttg ttgacgattt ccgggccgcc gcgcgcccat tttccgtgga agtagccatc
    18061 ttcgccgtct ttctggatca gacgcgccac atccgggctt ttacaactcc agcatgccat
    18121 cggtagcgga ccatcttcag cgtttttcgg cgcaccggta cgcagggttt cacgcacatc
    18181 ggtcacagca aaggcatgtc cacgcggctt gttgtaatcg cgcgagaagg gataccccgc
    18241 ccacaggatc accagccgtg gatcttccgc cagggcgtca acacgctctg actgttccga
    18301 ggtggctttc caggagagat attgatcggg atgttgcggg gcaaaggttt cattcttcgc
    18361 ttccacagtt acaggttttg cgggagtagc cgtttgttca gcgtgaacag aagtgaaaaa
    18421 gaaaaaagga atcaataagc tgaagatacg gcgtgcgttt atttttatcc ttgtcatagg
    18481 ggcttcatcc gaattgcgcc attgttgcaa tggcggtttt tattgttttt cacgacagta
    18541 accgcaccta cactgtcatg acattgctca cccctatgtg taacaaataa ccacactgtg
    18601 aatgttgtct ttaatcaatt gtaagtgcat gtaaaatacc actttagagt tagtcagtat
    18661 cttcctcttt ttcaacagca tgcataactg catgttcctc aaagaattaa tcaacttttg
    18721 ttgctgacct tcaaaaatta ccctgccgtt tatttgcaca attctacttt tgcgtgatct
    18781 gtcgcccaaa tactaaacaa aactgccaat acccctacat ttaacgctta tgccacatat
    18841 tattaacatc ctacaaggag aacaaaagca tgagtcaaat tcacaaacac accattcctg
    18901 ccaacatcgc agaccgttgc ctgataaacc ctcagcagta cgaggcgatg tatcaacaat
    18961 ctattaacgc accagatacc ttctggggcg aacagggaaa aattctcgac tggatcaaac
    19021 cttaccagaa ggtgaaaaac acctcctttg cccccggtaa tgtgtccatt aaatggtacg
    19081 aggacggcac gctgaatctg gcggcaaact gccttgaccg ccatctgcaa gaaaacggcg
    19141 atcgtaccgc catcatctgg gaaggcgacg acgccagcca gagcaaacat atcagctata
    19201 aagagctgca ccgcgacgtc tgccgcttcg ccaataccct gcttgggctg ggcattaaaa
    19261 aaggtgacgt ggtggcgatt tatatgccga tggtaccgga agctgcggtc gcgatgctgg
    19321 cctgcgcccg cattggcgcg gtgcattcgg taattttcgg cggcttctcg ccggaagccg
    19381 ttgccggacg cattatcgat tctaactcac gactggtgat cacttccgac gaaggcgtgc
    19441 gtgccgggcg tagtattccg ctgaagaaaa acgttgatga cgcgctgaaa aacccgaacg
    19501 tcaccagcat tgaacatgtg gtggtactga agcgtactgg cgggaaaatt gactggcagg
    19561 aagggcgcga cctgtggtgg cacgacctgg ttgagcaagc gagcgatcag caccaggcgg
    19621 aagagatgaa cgccgaagat ccgctgttta ttctctacac ctcaggttct accggaaaac
    19681 cgaaaggcgt gctgcacact accggcggtt atctggtgta cgcggcgctg acctttaaat
    19741 atgtctttga ttatcatccg ggtgatatct actggtgcac cgccgatgtg ggctgggtta
    19801 ccggacacag ttatttgctg tacggcccgc tggcctgcgg cgcgacaacg ctgatgtttg
    19861 aaggcgtacc gaactggccg acgcctgccc gtatggcgca ggtggtggac aagcatcagg
    19921 tcaatattct ctataccgcg cccacggcga ttcgtgcgct gatggcggaa ggcgataaag
    19981 cgattgaagg caccgaccgt tcgtcactgc gcattctcgg ttccgtgggc gagccaatca
    20041 acccggaagc gtgggagtgg tactggaaaa aaatcggcaa cgagaaatgc ccggtggtcg
    20101 atacctggtg gcagaccgaa accggcggtt tcatgatcac cccgctgcct ggcgctaccg
    20161 agctgaaagc cggttcggca acacgtccgt tcttcggcgt gcaaccggcg ctggtcgata
    20221 acgaaggtaa cccgctggaa ggcgctaccg aaggcagtct ggtgatcacc gactcctggc
    20281 cgggtcaggc gcgtacgctg tttggcgatc acgaacgttt tgagcagacc tacttctcca
    20341 ccttcaaaaa tatgtatttc agcggcgacg gcgcgcgtcg cgatgaagat ggctattact
    20401 ggatcaccgg gcgcgtggac gacgtgctga acgtctcggg tcaccgtctg ggaacggcgg
    20461 agattgagtc ggcgctggtg gcgcatccga aaatcgccga agccgctgtc gtcggtattc
    20521 cgcacaatat taaaggtcag gcgatctacg cctacgtcac gcttaatcat ggggaggaac
    20581 cgtcaccaga actgtacgca gaagtccgca actgggtgcg taaagagatt ggcccgctgg
    20641 cgacgccaga cgtgctgcac tggaccgact ccctgcctaa aacccgctcc ggcaaaatta
    20701 tgcgccgtat tctgcgcaaa attgcggcgg gcgataccag caacctgggc gatacctcga
    20761 cgcttgccga tcctggcgta gtcgagaagc tgcttgaaga gaagcaggct atcgcgatgc
    20821 catcgtaacc cacaattgcc ggatgcggcg tataacgcct tatccggtct acacgcccct
    20881 ttctttatag tgggaaaggg caaatataaa aattccccta agtatttcgc gttgcaggaa
    20941 ggaagcaagc gagcatctcc caggagcata gaaaactatg tgactggggg aggcatgtgc
    21001 ggctgacgaa cctgcggcgt gaatgacgac ggggaacctc tggagaatct gtgatgaatg
    21061 gtacgattta tcagcggata gaagacaatg cgcatttcag ggagttagtc gaaaaacggc
    21121 aacggtttgc caccatcctg tcgattatta tgctggcagt ttatatcggc tttattttac
    21181 tgatcgcctt cgcgcccggc tggctgggca ccccgctgaa tccgaacacc agcgtcacac
    21241 gcggtattcc gattggtgtt ggagtgattg tgatctcctt tgttctcact ggtatctaca
    21301 tctggcgggc gaacggcgaa ttcgaccgtc ttaataatga agtcctgcat gaggtacaag
    21361 catcatgaaa agagttctga cggcgcttgc cgccacactc cctttcgcag ctaacgccgc
    21421 ggatgctatt agcggggccg tagagcgcca gccaacgaac tggcaggcga ttattatgtt
    21481 cctgattttc gtcgtgttta cgctcggcat tacctactgg gcgtcaaaac gcgtacgttc
    21541 gcgtaatgac tactacaccg caggcggcaa tatcaccggt tttcagaacg ggctggcgat
    21601 tgccggggac tatatgtctg ccgcctcatt tttggggatc tccgcgctgg tgtttacctc
    21661 cggctatgat ggcttaattt actcgctggg cttcctggtg ggctggccaa taattctgtt
    21721 cctgattgcc gaacgtctgc gtaacctcgg tcgctacacc tttgccgatg tagcctctta
    21781 ccgcctgaaa caggggccaa tccgtattct ttcggcctgt ggttctctgg tggtggtggc
    21841 gctttacctt atcgctcaga tggtaggcgc aggtaaactg atcgagctgc tgtttggcct
    21901 taactatcac attgcggtgg tgctggtcgg cgtgctgatg atgatgtacg tcctgttcgg
    21961 cggcatgctg gcgaccacct gggtacaaat tatcaaagcc gttctgttgc tgttcggtgc
    22021 cagctttatg gcctttatgg tgatgaaaca cgtcggcttt agcttcaaca atctgttcag
    22081 cgaagcgatg gcggtacacc cgaaaggtgt cgacatcatg aaaccgggcg gactggtgaa
    22141 agatccgatc tccgcactct ctctggggct aggactgatg tttggtacgg ctggcttgcc
    22201 gcacattctg atgcgcttct ttacggtcag cgatgcccgc gaagcgcgta agagcgtgtt
    22261 ctacgccacc ggatttatgg gctacttcta tattctgacc tttattatcg gctttggcgc
    22321 gatcatgctg gttggtgcga atccggaata taaagacgcg gcgggccatc tgattggtgg
    22381 taacaacatg gcggcagttc acctggcgaa tgcagtgggt ggcaacctgt tcctcggctt
    22441 tatttcagcg gttgctttcg ccaccattct tgcggtggtt gcgggtctga cgctggcggg
    22501 cgcatcggcg gtgtctcacg acctgtacgc taacgtcttc aaaaaaggcg cgactgaacg
    22561 tgaagagctg cgggtatcga aaatcaccgt tctgattttg ggtgtgattg ccattatcct
    22621 cggcgtgctg tttgagaatc agaacatcgc cttcatggta ggcctggcgt ttgccatcgc
    22681 agcgagctgt aacttcccaa tcattctgct ttccatgtac tggtcaaaac tgaccacgcg
    22741 tggcgcaatg atgggcggct ggctggggct gattaccgca gtggtactga tgatcctcgg
    22801 cccgacgatt tgggtacaga tccttggtca cgaaaaagct atcttcccgt atgaataccc
    22861 ggcgctgttc tctatcagcg tggcattcct cggcatctgg ttcttttcgg caaccgataa
    22921 ctcagcggaa ggcgcgcgtg agcgtgaact gttccgtgcg cagtttatcc gctcccagac
    22981 cggctttggc gttgagcaag gccgcgcgca ttaatctctc cccttccccg gtcgcctgac
    23041 cggggaatac tcttcctctg caaaatgcat caccttttcc cataatatta aacaaataaa
    23101 cgcattaaaa aatgagcgat ttttgacagt cgtagaaaat gataatgcag agaatatgcc
    23161 ttttctttct tgttaattat aaggatattt tatgtgctac aatggtttaa ataatatgtt
    23221 tttccctctt tgccagatta acaataacca ctctgtcaca agtccatcac atacaaagaa
    23281 aacaaaatca gataattaca gaaaacatca taaaaacacg ttaattgaca ataaagccct
    23341 ctctcttttc aaaatagatg atcatgaaaa agtgataggc ttgattcaga aaatgaaaag
    23401 attttatgat agtttaccat caggaaaaat cacgaaagaa acggacagga aaatacataa
    23461 atattttata gatatagctt cacatgcaaa taataaatgt gatgatagaa ttacgagaag
    23521 agtttacctt aataaagata aggaagtgtc cattaaggta gtatatttta taaacaatgt
    23581 caccgtccat aataatacta tcgaaatccc acagacagta aatggtggtt acgatttttc
    23641 acaccttagc ctgaaaggca tcgtgattaa agatgaagat ttatccaatt cgaattttgc
    23701 aggttgcaga ctacaaaacg ctattttcca ggactgtaat atgtataaaa cgaattttaa
    23761 tttcgctata atggaaaaaa tactttttga taattgtatt ctcgatgact caaatttcgc
    23821 tcagataaaa atgactgacg gaactctaaa tgcatgctcc gctatgcatg ttcaattcgt
    23881 caatgcaaca atgaatagag ccaatattaa aaataccttc cttgattatt caaattttta
    23941 tatggcgtac atggctgagg taaatcttta taaagtaata gcgccatatg ttaatttatt
    24001 tagagccgat cttagtttct ctaaactcga tttaattaac tttgaacatg ctgatctgtc
    24061 tcgcgtcaat ctgaacaaag caaccctcca gaatataaac ttaattgata gcaaactctt
    24121 ttttacgcgg ctgacaaata cattccttga aatggttata tgtaccggct ctaatatggc
    24181 taatgttaat tttaataatg ccaatttaag caactgccat ttcaactgtt ctgttttaac
    24241 aaaagcctgg atgtttaata cccgtctcta tcgggttaat ttcgatgagg ctaacgtgca
    24301 gggaatgggc atttccattc tccgtgggga ggaaaatatc cccattgata gtgatatcct
    24361 ggtaacacta cagaaattct ttgaagaaga ttgtacctct catactggca tgtcacaaac
    24421 tgaggataat attcatgcag tcgctatgaa gattactgca gatattatgc aacacgcaga
    24481 ttgagattaa gccgaagaac agccaatgaa ttgactttaa aagagagata acttatttgt
    24541 tttagaatca tgtatgttag cccgagctaa cctgttaaag gtgcaaaaga ttgcaccttt
    24601 ttcaaattac ctcgggcctg gttacgttgc tttcggctta ctgattttcc tcaatcaaca
    24661 acgcttcaag caaatcgaga tcgtgcagta atttttgcag cgtttcgttg ctgatctccc
    24721 gcgtggcgcg caggtggtac agttcagcac gttcagaacg caatgccgcc agacggaagc
    24781 gacgctcaag gttctcttcc tgcacggaac tttcaacgtc gttacgtcca tcggcgcgac
    24841 gacgcaggtt accaatgacg cgagaactga cctccgtaag cagctggtta tcgatattct
    24901 cttcggtatc cgccgccaga cgctcctcca ttttctggat cgccacaatc gccacttctg
    24961 ccgttgccgc tcgcgcaata cgttcctctt tcaattgttg cgaatggtcc gcgacttcaa
    25021 tgtgttgtag cagaataggc aacatcacca cgccgacaaa cagcgagaag agaatgacac
    25081 cagccgccag gaataccagc tcatagcgcg ccgggaagac gttaccatcc ggcaagagca
    25141 gcgggataga gagcacaccg gccagagtga tcgccccacg caccccggcg aaagacgcga
    25201 tcaggatttc tcgtgtggtc cacgaaccaa actccatcgg cttcttcttc aggaagcggt
    25261 tgctgaactt tttcatcgtc cacagccagc cgaaacggac cagcatcagc gccgcataaa
    25321 tcagaatgat atcggtaaac agcatccaga tttcgacgtt agggtcgatt tctgccgcca
    25381 tcagcgacgt ctccagaata cccggcagct gcagacctaa cagcaggaat accatgccgt
    25441 taaagacaaa ttccagcatc gcccaggtac tgtttgcgcg caggcgcatt gccagcggcg
    25501 cacggcgcat cacaccggag cgggtgatgg tcatcccggc ggcaaccgca gcgaggatgc
    25561 cggaaacgcc aatatgttcg gcaatcagat aagaagcgaa tggcagcagg aacagcagga
    25621 cgatctgcgt cgcgggttca tcaccgcccc agcggctgag gaatcgcagc gagcgaccgt
    25681 acagccagct caccacaaaa ccggcgagaa taccgccaat ggctactttc ataaattcga
    25741 ccgtcgcacc gccaacggtg aagatcatcg tccccatcgc cactgctacg gcaaacttca
    25801 acgacaccag gccagaggcg tcattcatca acgcttcgcc ctgcaaaatg cccatgattt
    25861 ttttcgggat gcgcccttcg ccaacaatcc cggagagcgc cacagcatcg gtcggagaaa
    25921 gcaccgccgc cagcgcaaag gcggggatca gcggaatacc cggcaccacc cagtaaataa
    25981 ggaagccaat gccgaccacg gtgaccacca ccagcgccag cgcgaggccg aaaatctctc
    26041 gaccatgttc gagaaattca cgggtcggcg ttttccagcc atcagcgaac agcaacggcg
    26101 ggataaacaa gactaaaaag agttcaggat caaactccac atgcaaacca aacgtcggcc
    26161 acgccagtag cgcaccgatg gcgatttgca taagcggaag cgggatctga aagggcatga
    26221 cacgagtgac caccccggac agcgagacca cgagggtcat tatcagtatg gtgaagaaga
    26281 tttccatgcg ttccctgttt gcgatgtttt ttatgatctg agaaagacgt tatgcctgaa
    26341 ttctcatttt ttcagaatat cgcaaaagct cacacaatgt gcggagaaaa taaaaacggg
    26401 cagccattgc cacccgtttt cagaatcata tgaaagatta gatagcccag ccacccgcat
    26461 agaaggtcac cagcgccaca gcgataacga ccgtaccgat gttcaacttg cgccattcgc
    26521 cggaaaccag acgaccaatc accagagtcg cgaagccgat catgatgcct gttacgatgt
    26581 tacaggtcag cacgatgaat accgccgtaa ccagacccgc catcgcatca acaaaatcag
    26641 caaagtcgat tttcgccacg ttactcagca tcagcaggcc aacgtacatc agcgccggag
    26701 ccgttgcata ccccggaacg aggtaagaga gcggagagag gaacagaatc agcaggaaca
    26761 gcacgccaac ggtgatagcc gtcaacccgg ttttaccgcc cgccgccgta cccgccgcag
    26821 actcgatgta taccgctgcc ggagctgcac ccaccaggcc agagaaaacg ctgctcatgg
    26881 agtcggtggt cagtgctttc ccaccgtcaa tgatctgccc gtctttatcc agcaggttcg
    26941 cctgaccggc gacggcgcgg atagttccgg tggcatcaaa tactgccgtc atcaccagcg
    27001 ccagaacgct tggcaggact acaggattca gcgcgcccat aatgtccagg ctgccaatca
    27061 gtgaattgcc gttttcatcg ctcaatgaag gcatggcgaa aacgccggag aaatggacgt
    27121 taggatcgaa gatcaaaccg acaattgaga taccgataat ggtcagcaga atgccaccag
    27181 ggactttcag tttttccagg ccgatgatca ccgccagacc taccagtgac ataatcaccg
    27241 ggaaggtcgc gaaatcaccc agcgcaacgg gcagaccatc aagcgggttt ttaatcacca
    27301 gaccgacacc attagcggca atgagcagca ggaacagacc gataccgatc cccgtgccgt
    27361 gcgccacacc gtgcggcaag ttgcgcaaaa tccagctacg gatacccgtg gcagaaatta
    27421 ccgtaaacag tacacccatc aggaacacag cacccagcgc gacaggtacg ctaatatgtt
    27481 gccccagcac cagactgaat gcggtaaacg ccgtcaggga gatggcgcaa ccaatcgcca
    27541 acggcagatt agcccacaga cccatcacga tagaaccgag tccggcaacc agacaggtcg
    27601 caacgaaaac tgccgcaggc gggaagcccg ctttacccaa catgcctgga acgacgataa
    27661 ccgagtagac catcgccaga aacgttgtta acccggcaac cacttcctga cggacagtgc
    27721 ttccacgttg tgaaatttta aaccaggcgt cgagtgaacc gccggtacgc gctgatggcg
    27781 tagacataag agaacatccc ctgagagttt aattttcgtc aagatgcgtg gcggacaatc
    27841 ccctgccagc gaaacgttat cttcctgctc caggtttgcg acaaattggt gcgtcgcaaa
    27901 aaagcaaacg tttagctccg cgcatacaaa acgggtggta aaaaaagcaa acgattatcc
    27961 ggcgatcata gcggcttttt caaccgaagt ttgctgcttt tttctctttt attgtcttga
    28021 cgatgaaaaa ccaggcaatt gatgcgcaaa cgttatcgtc gatgcgcaag attgcagcgt
    28081 tatcgagccg cataccgttc aggattgcga taaaaaatcg tctgagatga caaaaatatt
    28141 gcggctaaaa agtgtacttt tacactggcg aagaaacgcc ggtgacatac aattaaagca
    28201 tcaacaccaa ccggaacctc caccacgtgc tcgaatgagg tgtgttgacg tcgggggaaa
    28261 ccctcctgtg taccagcggg atagagagaa agacaaagac cggaaaacaa actaaagcgc
    28321 ccttgtggcg ctttagtttt gttcatcttc cagcaagcgt gcgcctgtgc cttcttctcc
    28381 taagcggtcg cccgggttac gcaatgggca atcactgcgc gaaaggcagc cacaaccaat
    28441 acatccgtcc agctcgtcac gcagcgccac taaggtatga atgcgccgat ccaactcttc
    28501 tcgccattgg gacgaaagct gtttccactc tttcgcactt aatgtatgcc cttcgggcaa
    28561 cacgccaaac gcttcacgaa tggtcgccag aggaatgcca atgcgctgag caattttgat
    28621 gattgcgaca tatcgcaaca catcacgttt atatcgccgc tgattgccac tattacggat
    28681 actgctaatc aaccctttgc tttcatagaa atgcagcgcc gataccgcca caccgctgcg
    28741 ttttgccact tcgccggggg ttagcagcgc tttaatgcgg ggtaatttct tttccataaa
    28801 tcgctttacc tcaagttaac ttgaggaatt atactcccca acagatgaat taacgaactg
    28861 aacactgaaa agaggcagat ttatgtccca tcagaaaatt attcaggatc ttatcgcatg
    28921 gattgacgag catattgacc agccgcttaa cattgatgtc gtcgcaaaaa aatcaggcta
    28981 ttcaaagtgg tacttacagc ggatgttccg cacggtgacg catcagacac ttggtgatta
    29041 cattcgccaa cgccgcctgt tactggccgc cgttgagtta cgcaccaccg agcgtccgat
    29101 ttttgatatc gcaatggacc tgggttatgt ctcgcagcag accttctccc gcgttttccg
    29161 tcggcagttt gatcgcactc ccagcgatta tcgccaccgc ctgtagtttt attgcccgcg
    29221 cgttaactcc cgcgcgggta attgctccat ccattgcata aacacctgcg gcggcatcgc
    29281 cttcgcaaag aaccatccct ggcaatagcg cacgccgcgt ttgcgcagcc agttaacctg
    29341 ctcctcagtt tcgacgcctt cagcgatcgt ttttaacccc agactgtgcg ccagctcgat
    29401 gatgtgttcc gcaatcaaat gactggtttt atgggtggtc agcgtctcaa caaacgattt
    29461 atcgattttc aaaatatcga cattcaatga tttaaggtta tgcaagttag agtagccaat
    29521 accaaaatca tcaattgcca cttcgtaacc agcctggcgg aaagccagaa taattggtgt
    29581 cattttgtcg acatcaagaa atgcatgttc agtcacttca aatttaattt gctgtggacg
    29641 caccgcgtat tgctctgttt tctgattggt tcgcgctatc aaccgtgacg tatgaaaatc
    29701 ggaggccgac aggttaatag aaacatagcg atcggcatgt gttgccaggt acgcgcccag
    29761 atcgcggaag acattatcaa taacataatc agttacctgt ccgatcatcc cctccttttc
    29821 tgccagcgga ataaactctg ccggattcat tacttgcccc tgctcaccag gccaacgtaa
    29881 caatgcttca gcgccgatac atttttctgt tttgatatcg attattggct ggtaataaag
    29941 gcaaagttga tgtttttcga gggcgcgttg caatttgcgt ttgggagaca aatagttttg
    30001 ccgaatacgt agccagagta ataacagaac aagactcccc aaaataccgg cgggtaacgc
    30061 aaaaataaga tgattataaa aatgcgctat aagacgttga tatgaagtcg caacaatggc
    30121 tgcaattggg cgttttgtcg aataaactgt cgcatataaa tagccatttc tttgtacggt
    30181 taaatcattc aaatgaatca gtggagaaaa cgttgctgcc gaggcctcgt tgcttaacga
    30241 gaaaaaggtt ttcgttaccg tatcatacac accccattgc aatgtcggat catcagacat
    30301 cacttcactc cagaagagag ggttgatgac caccacataa tttccccgct gcatataggt
    30361 cattttatag ccagagaaaa aaggcgtatc gcggtaataa tagatagaaa cgttaggttc
    30421 acgcttataa tcggccggtg caatcgtata gccgtttaca ggcgctatca gcgatgagca
    30481 taaaaaatgg ttatcacggg catagatcaa ttcattaata taaagatagc cacgaataat
    30541 attcaacatt cgcttttgat gggctggagt gcaaacttgc ccctgatagc gttctgctgc
    30601 atcgctcact aaatctgcct ggcgaatgac cagctcggat ttatccagcg ccagctgagc
    30661 aaaagtttgg agctgactat tcacttctga ttttgcccaa aggaaggcaa tccatagcga
    30721 cagtatgatg ggaaagagaa ctaaaaagat aatgcccggc aacgccagta attggtgtcg
    30781 tgcacgatga ctcattgcta tcaatttcct gcctgtttat acccggtcat tgcaaggcga
    30841 gaaccctggt tatcgacgtc tttagataaa tcttacggta taaatagcac ttttgcctga
    30901 tttgaaattg ataccttttt aatcgtcacc tgaataaaaa taattcattg atattcaaaa
    30961 ctaatttcct acaggaatgc agaggcggtg ggaagatatc cccttaatgc cgctgaaaaa
    31021 acacataatc tgtacggcaa taaaagtcac ggcctaatct ggctaacaaa caatcaattg
    31081 tgatatagtt cacaaaatta atgaaacaaa cagagtgttt cttttttgtg ttctgcaagc
    31141 cccgcgatag ccagattgta tcacgcctcc cgtgaacaac gccgcgctgt ccgaggcatc
    31201 ggctcttttc agaggaattg tttgatggct accctcacca ctggcgtggt tcttcttcgc
    31261 tggcaacttc ttagtgccgt aatgatgttt ctggccagca cactcaacat ccgttttcgt
    31321 cggtctgatt atgtcgggct tgcagtgatc agcagcggtc tgggcgtggt ttctgcctgc
    31381 tggttcgcaa tggggttgct tggcatcaca atggcggata tcaccgccat ctggcacaac
    31441 atcgagtcgg tgatgataga agagatgaat cagacaccgc cacaatggcc aatgattttg
    31501 acttgatatg tagaagcctc caaaacggag gcttcttttt tacggctggc agatgcttta
    31561 atcatccacc ttaaaacaat ataacctatt gttttaatga caaatcagaa cggttctgtt
    31621 tacctgatta taaataaaaa cattaatttc aataagttaa gttaaaaaat aatagttatg
    31681 taatgttttt agttaaattt actctttatg acgactaaaa attttacatt cctcgccgat
    31741 tgatgaaact tacttcttaa ccgcggtttt ctgactcagt agcactaaga ataatgagtt
    31801 agcgtcaaag gctatgctaa cctattcaaa aaaagcctat agaatccaag aatgtggata
    31861 aggactaaga aattaatgag cttctcatca actcatacga ggtggctgac caagaatgaa
    31921 gaggacctaa gaaccgcaga cggcaaaaat atcgaagtct gggttttccg tcatgaacaa
    31981 gacgaacaag tgctttcaga atgggcaaag cactttcgga accactactg ctttgacagt
    32041 gaaatcgatt atttccgtag gggatacggg tattctcgcg aagactatct caacctcata
    32101 aagtttccag acataaaaaa tgcgcctggt ccaagcattc gtgcaggtga ctttggagaa
    32161 gtattggtgg ctgattttat tgaatacttg ctcggctact gggttcctcg cactcgctac
    32221 ggtgacaaaa ctattcgaaa cgagtcgact aaaggcagtg acataatcgg ttttcatatc
    32281 atcaaggacg gtattgtatc ttcgaatgat aggttagcga tctttgaagc gaaagcacaa
    32341 ttctcaggga aaaaagcgaa gccgagactt caggatgcag tggatggctc attaaaagac
    32401 gttgcacgga aggcagagtc tttaaatgct attaaacaaa gactgtatgg tcgtaatcag
    32461 cttgatgatg ctgaaaaaat tgaacgcttc cagaacgaag tagattatcc atataaggaa
    32521 gtatatggtg ctgtagcgct ctttgatagt ccactttttg acagccacct tacatcattg
    32581 accgatacca tttctcatcc atattcaggt gatcttgtgt tagttgtgat cacaggggat
    32641 cagatgatgg cactagtcca cgagctctat aggagagctg cgcatgaggc ctgaaaagaa
    32701 atcgcaactg ctgcttggcg tctcccggtc aaaggcaaag atgcttgagt tcagcattcc
    32761 ggaagagcat cacatcaaaa taacccaaga tccggccaag cttttcagca tatctattgg
    32821 tctactggga gatctcgcag ctgccataaa cagagaggag ccagaggtta atttgctcac
    32881 agagatgaaa accaatcttt tattttctgc cagctttttc gactcttacc tccagtcaaa
    32941 gttaaatgaa acgcttgacc catatcttat gcttcttggt gcagcctcat actatttatg
    33001 cgaactccca ggtagttcat cagttttgat aagaaaattg aaaagtgata ttgatgcatc
    33061 tggcatttat ggactgtact gtccaaatct agatggagcc gggctggaat ttttattgct
    33121 gtggttgctt gaaggagatt caagcattca ttttgagaat atcgactcag catttggaaa
    33181 acccatagaa aacatttcta aagagttaaa tatatttttt tcaactggaa atgacttcgc
    33241 aattcgaagt gaggtgaaaa ggctaaggga tttagcttac tttgctggca ctccaaggca
    33301 gttactttta agcgacttga tatcttcaat aactttgaaa aaaatagata actcaacgtg
    33361 gaagtgctta ccaaaatata gtggcctatc aattagtacc tggatggaag cactgcataa
    33421 aaatagcttc attaaagaat tttggccagc acagcattta ttgggacaag ccaatgttct
    33481 aagaggaaag tcagccatcg ttcaaatgcc caccagtgca ggtaagacaa aggcaactga
    33541 gctaattctt cggagcgcgt ttttagccaa acgtgtgtca cttgctatta tcattgcccc
    33601 atttagagcg ctatgtcatg aaatcaagaa cagcctcgtc gaagcattca acagcgaatc
    33661 aaccaaggtg gatgagttaa ccgataccct acagactgac tttgagatcg ctgaactctt
    33721 ggggcaccaa cagatcctgg tcgtaacacc cgagaaacta ctttacgccc ttcggcacgc
    33781 tccggagcta ggggcacaca ttggcctatt aattcttgat gagggacacc agttcgacag
    33841 cgggactcgg ggcatcacat acgagctact tctgacgtca ctacgcacta tgattcctga
    33901 aggaacccaa aaggtcctaa tctcagcagt catcagcaat gccgaggctg ttggattatg
    33961 gctaaacggg gaaccgaatg tcgtcgaagg cacaaccctt attccaactt tcaggtcgat
    34021 cggattcgcg agctggctcg atcaactggg aagaatcgaa tacgtcgaca gtcgcaacgc
    34081 tgaacaaagt gagtttttcg tcccgagagt gattgaaaca tttaatctcg gaaggaagcc
    34141 acgagaacat actgaccgtt tttttcctga aaaaaataat ggacaggcta tcgcacttta
    34201 cctcggtcta aaattggcac caaagggcag catagctatt ttctgcgggc gaaagtcaac
    34261 tgcggcaagc atctgcgaaa aggcagtcga tattatcaag cgaggtgtac cactgccctt
    34321 accatccgat tattcagatc cccaagaagt ggctcggcta acacatctgc atatcaaaaa
    34381 tctcggggct aacgctccag catctcagag tgcggaacat ggaatttttt cccatcatgg
    34441 caatacacca caaggcattc gactggcagt agagcatgct atgcgtgaca atcttgtgcg
    34501 attcgttgtt tgtacatcta ccttagcgca gggtgtaaat cttccgatac gatacctcat
    34561 cgtgaccagt gtctaccagg gtatggagcg catcaaggtt cgtgatttcc acaatcttat
    34621 tggtagagcg ggtcgagccg gaatgcatac cgaaggcagc atcctgtttg cggatccagt
    34681 aatctttgat aagcggaagg tgctgagcga aaaatggcgc tggaagcagg tgaaaaaact
    34741 actggaacca caaaattctg aaccatgtgt cagcaacctt ctatcaatat ttgatcccat
    34801 caagagcgat gacgagaaaa tctcaatccc catgggggca ctagacttta ccaaggccta
    34861 tatcgacaat cccgaaaaaa ttgcgaatct tgccacagag ataaccgcat tgcatggaga
    34921 taaaaatttt tctcaaaaca atgttgagcg tcaaatagcc tggagaatca gcctaatatc
    34981 tgcagtcgaa agtttccttc tatcccattg ggatgaatcc gaagatggtt tatcgcaagc
    35041 tgacgtgacc cacctagctg aaggtacgct agcttttttt ctcgcagaca ctcagaaaaa
    35101 ggagcatata cgcgagttat ttcaactgct cgcaaggcac atttcaacaa atattaccga
    35161 gccgtcccgc cgaaaaatct atggtagaac gctttacggg attcaggatg ctcaatctat
    35221 tgagaaatgg gtccaatcca acactgacag ccttctttct attgttgatg aaaaagaaat
    35281 actcaaccat atttggcaat tgcttatcca gcatatcaac agtggtgtat ttaccaagtt
    35341 tgatgaacca gaggtgctta aagaaatatc gtatgaatgg atcagtggca aacccttcaa
    35401 cgacctccta gaaattatcc atgaacgaaa agttaagatg atatggggca ctaatcggag
    35461 agagttcaag atcgaccata tcgtagatat ctgcgaaggg gcgcttgcat ataatgcaac
    35521 gctcgttgta ggtgcaatat ctgagttcat cgaaactctt aataaggatg gcacaaggga
    35581 tctgttcaat cgtctgcagc ttttacaaaa aaaactaaaa tatggtcttc caacagacac
    35641 cgctatttcc ctatatgagc ttggtttttc agatcgcgta atagctcagg atctcgccac
    35701 gtccttgaag ctagcggcga ctcagaagaa atatctcgtg aaagagttga aaaataatcg
    35761 aggtaatgca atgaaggtga tagaaaaata cccaatgtat ttccaagaaa gaatgtcaga
    35821 gttgatagaa gatttctaaa taaagaatcg atattgaaag ttagtataaa gcacgagcat
    35881 aatcctcatg ctttatgcta acaataggag gagatgttac gaaataaaac tctacacaat
    35941 tttaagataa gtgtataaga tcatatatag ttcagattgt taatagaaat tgataccata
    36001 agcataacta ggttaaatga tggtgtaaaa gtgaataaac agattgatgt tggactccat
    36061 gtgctgatac ggacgcggta taacgccgcg ttagaaaagt ggagtaaatt tatatggtta
    36121 gagataggtc ttggcgacga cagcatgcct ttcgtttgaa atcgaagaga agattttatc
    36181 gtattgcagt aactaaaaca tcaattacta taggtaaaat ctatcagtca ccttgtgcat
    36241 gctcttgtta tctttgtggt aataaaagaa agaaccatgg cgaaacaatg caagaacgcc
    36301 gtgcaaagtt actgcacacc gattaattta tattaagtat ttaggataac cgtcaggcta
    36361 gtctttgacg cccttgtacc tatactactt tagtctcaac aaccccctac cacataggcc
    36421 aaaagctgtg tagtaggggg ttatctcaac ggaaagactc aggcggatct cgccaagtat
    36481 aactcccatg catagggcaa tcatacccgt tagcgctggt gtgtgcggct gccaacagcc
    36541 attaagggtg cctgatgcgc ttcctctcag tatacagcgt tgctaagttt cgcgtccagc
    36601 tccttcgcaa aatcaaagtc attccatttt gtaaaccaat attcatttaa tgaaataatt
    36661 cgttttacat acgatttttc aataccataa cgagaaagca gagctttttt aaaagaaaca
    36721 gatatgtatg ggcaagcaat tagatccaac gttaagtgaa ccaattcaga ctcatttaga
    36781 agagtatcaa ctttcatgct ataaatattt tcaatatgaa gcaaaagatg cttcctgacc
    36841 ccatcatatc tttttttgcc actcatataa aacattagag aagtaatgga aaagtaattc
    36901 aaattatcat caaatgagta ggcgccacct tcaaagctaa tattgaaata cttcatcaag
    36961 cttttctcac ctaaccaata atatttacca agctgcctaa tcagcactaa caaatacaaa
    37021 gtttcgattg gtgttgtttt atcaacggaa tatttatcta aaatagaaca cgaattatcg
    37081 aatacacatt tgaatacaat atgttgatat tcttcgcata ttttttgatc tttaacaaac
    37141 ataattactt gctgaattat cctacataat tttatagtgc tattcacttt aggagcaact
    37201 gcatatataa aatatgtgaa attcaccaca gatataatag cattaattag ttgacgctcg
    37261 ccactttcat cttttgaaaa ctctttataa tctttaaaaa gtgtctttat tttcctttca
    37321 atgatagaca gtgaatagtt taagatatcc gaatatgtta tacctgatac agaaagtatc
    37381 gatttaaagt cggtagataa actattttga tgtatgtaga cattcccttt cttaatatct
    37441 ccttctagag agccattgtc agtaatgtta taagatattc tttccgatat caaattagct
    37501 atttttttct tggctatagt tattggtgtt attattggac gcgaaaatct ctcttcttta
    37561 tatttattca aacttagctt gaaggttttt agatttttct gtgtgcttat tctaatttta
    37621 ttgaatacat catcgtcatt ataaaaaatg aaatagtcat cgacatagcg atatacttgg
    37681 taatccccac caagaactaa accgtcttta ttaagatcat tgatgatatt gttatccact
    37741 gactgcatta ttatctctgc aaaaattctg gataattccg gcccaattat tattccattt
    37801 gtttcgttgt agttcgacct ctgcatcaat tgatcaaatt taaatgggaa tgtcgactgt
    37861 gtcttattta actgttgttt tgagtaattt ttttgcaaaa cagcccatga aattgaatgg
    37921 gtgtaaattg aatcaaaaca gttcgacaca tctaatttag agagaaaatt aaaacgcttt
    37981 tcacaccaat gatgatcttc agattcataa aattcaaaaa tattacgata tttcttataa
    38041 acaaaaaatg attttaaatt ttcataatcc tgaccttcaa cctcaatact taaatcttct
    38101 ttctcaaaac tttcaattag agactttgaa tcgatgtaag tacaaccagc gatccttttt
    38161 gctgatctga ggctaaatgg gctaacattt gaatagtatg ttattaaatc tttataattc
    38221 ctatagaaat caacgcctaa tagctgtcct ttaggatgca ttagagacaa gtatctgtat
    38281 tctttatgtt tatgcgaaat tggaaaagta aaagcaatgc taggcagttt agcatacttg
    38341 atatttgttt tagtataact aataccctta tcaactattt caactgtttc cgtgttctgt
    38401 tcaagaccca ataaaagtag aattaaacta tttaaacaac catctctctt tagccatatg
    38461 aaattcaacc catcatgttt tatttgatgc ttaacaataa acttgtaaaa atgtctattt
    38521 gaaaaaataa tagggacttc atatggtaaa aaatcggaaa gaactactcg ttctattggg
    38581 aaatttatcg ataacgcttt tttagacatg tgaccatccc ttagatatat cgctaaattc
    38641 tatgaaacta
//
LOCUS       DAPOZO010000045        38470 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPOZO010000045 DAPOZO010000000
VERSION     DAPOZO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.045"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            234..1277
                     /gene="nadA"
                     /locus_tag="ROI21_002419"
     CDS             234..1277
                     /gene="nadA"
                     /locus_tag="ROI21_002419"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDX5111331.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKCLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1315..2034
                     /gene="pnuC"
                     /locus_tag="ROI21_002420"
     CDS             1315..2034
                     /gene="pnuC"
                     /locus_tag="ROI21_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDX5111332.1"
                     /translation="MGFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2031..2972)
                     /gene="zitB"
                     /locus_tag="ROI21_002421"
     CDS             complement(2031..2972)
                     /gene="zitB"
                     /locus_tag="ROI21_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDX5111333.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3086..3466)
                     /locus_tag="ROI21_002422"
     CDS             complement(3086..3466)
                     /locus_tag="ROI21_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDX5111334.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3781..4833
                     /gene="aroG"
                     /locus_tag="ROI21_002423"
     CDS             3781..4833
                     /gene="aroG"
                     /locus_tag="ROI21_002423"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDX5111335.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAARLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            complement(4999..5751)
                     /gene="gpmA"
                     /locus_tag="ROI21_002424"
     CDS             complement(4999..5751)
                     /gene="gpmA"
                     /locus_tag="ROI21_002424"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDX5111336.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(5954..6994)
                     /gene="galM"
                     /locus_tag="ROI21_002425"
     CDS             complement(5954..6994)
                     /gene="galM"
                     /locus_tag="ROI21_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDX5111337.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPLDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWHGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(6988..8136)
                     /gene="galK"
                     /locus_tag="ROI21_002426"
     CDS             complement(6988..8136)
                     /gene="galK"
                     /locus_tag="ROI21_002426"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDX5111338.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8140..9186)
                     /gene="galT"
                     /locus_tag="ROI21_002427"
     CDS             complement(8140..9186)
                     /gene="galT"
                     /locus_tag="ROI21_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDX5111339.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESKDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDGQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9196..10212)
                     /gene="galE"
                     /locus_tag="ROI21_002428"
     CDS             complement(9196..10212)
                     /gene="galE"
                     /locus_tag="ROI21_002428"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDX5111340.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10475..11947)
                     /gene="modF"
                     /locus_tag="ROI21_002429"
     CDS             complement(10475..11947)
                     /gene="modF"
                     /locus_tag="ROI21_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDX5111341.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIN"
     gene            complement(12015..12803)
                     /gene="modE"
                     /locus_tag="ROI21_002430"
     CDS             complement(12015..12803)
                     /gene="modE"
                     /locus_tag="ROI21_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDX5111342.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            12932..13081
                     /gene="acrZ"
                     /locus_tag="ROI21_002431"
     CDS             12932..13081
                     /gene="acrZ"
                     /locus_tag="ROI21_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDX5111343.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13247..14020
                     /gene="modA"
                     /locus_tag="ROI21_002432"
     CDS             13247..14020
                     /gene="modA"
                     /locus_tag="ROI21_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX5111344.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQNI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVIAFYDYLKGPQAAEIFKRYGFTTK"
     gene            14020..14709
                     /gene="modB"
                     /locus_tag="ROI21_002433"
     CDS             14020..14709
                     /gene="modB"
                     /locus_tag="ROI21_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDX5111345.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14712..15770
                     /gene="modC"
                     /locus_tag="ROI21_002434"
     CDS             14712..15770
                     /gene="modC"
                     /locus_tag="ROI21_002434"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDX5111346.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITALFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15771..16589)
                     /gene="ybhA"
                     /locus_tag="ROI21_002435"
     CDS             complement(15771..16589)
                     /gene="ybhA"
                     /locus_tag="ROI21_002435"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDX5111347.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARACEAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWQDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIARFIYSHLI"
     gene            16744..17739
                     /gene="pgl"
                     /locus_tag="ROI21_002436"
     CDS             16744..17739
                     /gene="pgl"
                     /locus_tag="ROI21_002436"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDX5111348.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17780..18733)
                     /locus_tag="ROI21_002437"
     CDS             complement(17780..18733)
                     /locus_tag="ROI21_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5111349.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            18917..19969
                     /locus_tag="ROI21_002438"
     CDS             18917..19969
                     /locus_tag="ROI21_002438"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDX5111350.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVFLP"
     gene            20045..21478
                     /locus_tag="ROI21_002439"
     CDS             20045..21478
                     /locus_tag="ROI21_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDX5111351.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            21661..23922
                     /locus_tag="ROI21_002440"
     CDS             21661..23922
                     /locus_tag="ROI21_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDX5111352.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDMLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQITGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(23992..25275)
                     /locus_tag="ROI21_002441"
     CDS             complement(23992..25275)
                     /locus_tag="ROI21_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDX5111353.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DSDEIQRNMNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(25427..25903)
                     /locus_tag="ROI21_002442"
     CDS             complement(25427..25903)
                     /locus_tag="ROI21_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDX5111354.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(25962..27251)
                     /gene="bioA"
                     /locus_tag="ROI21_002443"
     CDS             complement(25962..27251)
                     /gene="bioA"
                     /locus_tag="ROI21_002443"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDX5111355.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHADIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVVAIEVQLREQLAPARDAKMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            27338..28378
                     /gene="bioB"
                     /locus_tag="ROI21_002444"
     CDS             27338..28378
                     /gene="bioB"
                     /locus_tag="ROI21_002444"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDX5111356.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            28375..29529
                     /gene="bioF"
                     /locus_tag="ROI21_002445"
     CDS             28375..29529
                     /gene="bioF"
                     /locus_tag="ROI21_002445"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDX5111357.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            29516..30271
                     /gene="bioC"
                     /locus_tag="ROI21_002446"
     CDS             29516..30271
                     /gene="bioC"
                     /locus_tag="ROI21_002446"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDX5111358.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRYPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            30264..30941
                     /gene="bioD"
                     /locus_tag="ROI21_002447"
     CDS             30264..30941
                     /gene="bioD"
                     /locus_tag="ROI21_002447"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDX5111359.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            31520..33541
                     /gene="uvrB"
                     /locus_tag="ROI21_002448"
     CDS             31520..33541
                     /gene="uvrB"
                     /locus_tag="ROI21_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDX5111360.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(33579..34487)
                     /gene="yvcK"
                     /locus_tag="ROI21_002449"
     CDS             complement(33579..34487)
                     /gene="yvcK"
                     /locus_tag="ROI21_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDX5111361.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLNEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      34752..34896
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            34884..35873
                     /gene="moaA"
                     /locus_tag="ROI21_002450"
     CDS             34884..35873
                     /gene="moaA"
                     /locus_tag="ROI21_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDX5111362.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            35895..36407
                     /gene="moaB"
                     /locus_tag="ROI21_002451"
     CDS             35895..36407
                     /gene="moaB"
                     /locus_tag="ROI21_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDX5111363.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            36410..36895
                     /gene="moaC"
                     /locus_tag="ROI21_002452"
     CDS             36410..36895
                     /gene="moaC"
                     /locus_tag="ROI21_002452"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDX5111364.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            36888..37133
                     /gene="moaD"
                     /locus_tag="ROI21_002453"
     CDS             36888..37133
                     /gene="moaD"
                     /locus_tag="ROI21_002453"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDX5111365.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            37135..37587
                     /gene="moaE"
                     /locus_tag="ROI21_002454"
     CDS             37135..37587
                     /gene="moaE"
                     /locus_tag="ROI21_002454"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDX5111366.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            37724..38428
                     /locus_tag="ROI21_002455"
     CDS             37724..38428
                     /locus_tag="ROI21_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDX5111367.1"
                     /translation="MDRFPRSDSIVQLRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYISVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
BASE COUNT         9150 a         9643 c        10243 g         9434 t
ORIGIN      
        1 ctctcaattt caattcttaa ccctgatgac atactccgcg cttgctgcgt aaacaactct
       61 acgtcggagg aataagacgc aaagtgtggc attcgtcatc tcatacgtaa ttcgagtgac
      121 ttttccccac cattcgacta tcttgtttag catataaaac aaatttcacc gataacagcg
      181 aatattacgc tcatgtcggt tttaacgtta agcctgtaaa acgagatggt aagatgagcg
      241 tgatgtttga tccagacacg gcgatttatc ctttcccccc gaagccgaca ccgttaagca
      301 ttgatgaaaa agcgtattac cgcgagaaga taaaatgtct gctaaaagaa cgtaatgcgg
      361 tgatggttgc ccattactat accgatcccg aaattcaaca actggcagaa gaaaccggtg
      421 gctgtatttc tgattctctg gaaatggcgc gcttcggtgc aaagcatccc gcttctacgt
      481 tgttagtcgc tggggtgaga tttatgggag aaaccgccaa aattctcagt ccggaaaaaa
      541 caattctgat gccgacactt caggctgaat gttcactgga tctcggctgc cctgttgagg
      601 aatttaacgc attttgcgat gcccatcccg atcgtactgt cgtcgtctac gccaacactt
      661 ctgctgcggt aaaagcgcgg gcagattggg tggtaacttc aagcattgcc gtcgaactta
      721 ttgatcatct ggatagtttg ggtgaaaaaa tcatctgggc acccgacaaa catctggggc
      781 gttacgtgca aaaacagacg ggcgcagaca ttttatgctg gcagggtgcc tgcattgtgc
      841 atgatgaatt taagactcag gcgttaaccc gcttgcaaga agaatacccg gatgctgcca
      901 tactggtgca tccagaatca ccacaagcta ttgtcgatat ggcggatgcg gtcggttcca
      961 ccagtcaact gatcgctgct gcgaaaacat tgccacatca gaggcttatt gtggcaaccg
     1021 atcggggtat tttctacaaa atgcagcagg cggtgccaga taaagagtta ctggaagcac
     1081 caaccgcagg tgagggtgca acctgccgca gctgcgcgca ttgtccgtgg atggccatga
     1141 atggccttca ggccatcgca gaggcattag aacaggaagg aagcaatcac gaggttcatg
     1201 ttgatgaaag gctgcgagag agggcgctgg tgccgctcaa tcgtatgctg gattttgcgg
     1261 ctacactacg tggataacga ataataaggc gtaacgttac gctttggggg aaagatgggt
     1321 ttttttagtg tgcagaatat cctggtacat ataccaatag gagcaggcgg ttatgatctc
     1381 tcatggatcg aagcggtagg cacgatcgcc gggttgctgt gtattggcct tgccagtctg
     1441 gagaagatca gcaactactt ctttggcctg atcaacgtca ccttgtttgg cattattttc
     1501 tttcagattc agctgtatgc cagcctgcta ttacaggtgt ttttctttgc cgcgaatatt
     1561 tacggttggt atgcgtggtc gcgacaaacc agtcagaacg aggcggagtt gaaaattcgc
     1621 tggttgccat tgccgaaggc gctaagctgg ttagcggttt gcgttgtttc gattggtctg
     1681 atgacggtat ttatcaatcc ggtatttgca tttttgaccc gcgtggcagt catgatcatg
     1741 caagcattag gattacaggt tgcgatgcct gaactgcaac cggacgcttt cccgttctgg
     1801 gattcctgca tgatggtgtt atctatcgtg gcaatgattc tgatgacgcg taagtatgtg
     1861 gaaaactggc tgttgtgggt gattattaac gtgattagcg tcgttatttt tgcacttcag
     1921 ggcgtttacg ccatgtctct ggagtacatc atcctgacct ttattgccct caacggcagc
     1981 cggatgtgga tcaacagcgc acgcgaaaga ggttcacgcg cgctgtccca ttaatggtga
     2041 tgatgtgaat ggccagacac cccttcattc aggtggcagt ctggcccatg acaaggttga
     2101 tattccatct gaatagtggc gtgctcaatc tgatagtgat ccatcaggta gtgttggatc
     2161 tgatctaaca aggcatcgtg atcgtgtggc gggatcacct gcacatgcag cgtcatcacc
     2221 ggcttctcgc ctaccatcca tacatgtaca tggtgcacat tgcggacttc cgggatttcc
     2281 cggcacatac gacgcttcag ttcggcgata tccagcgata ccggtgcacc ttcaagtaat
     2341 tcattcacgc tatctttcaa cagtcgccac gcgctacgca gaaccagaag cgacaccagt
     2401 attgagagaa tggggtcagc aggtgtccag ccggtccaga taataatcaa cgcggcaata
     2461 atagccccaa ccgaacccag caggtccccg agcacatgca gtgccgctgc tcgcacgttg
     2521 aggtttttct cttcgctgcc gtgatgaagt aaccagaaag aaagtatatt tgccagcagc
     2581 ccagccacgg caattgccat catcatgccg ccctcgaccg gacgcggagt gcggaagcgt
     2641 tctatcgcct cccagacaat caaaatagta atcaccacca gggcgatggc attcacaaac
     2701 gccgcgagcg tggtcaatct tagccaaccg aaagtgtggc gaatggtggg aggacgacgg
     2761 gaaaattgca ctgcgagtag ggcgaaaagc agggcggcag tatcggtcaa catatgaccc
     2821 gcatcggcca gcaatgccag agaaccggaa agaaaaccac caatgacttc tacgagcata
     2881 aacccggcag tcacgccgaa agcatacaac aagcgacgag cattattatc ttctggcagg
     2941 tgtgaggaag tgtgtgagtg tgagtgcgcc atgacgagtc atcccttaat tattctcact
     3001 taactttatg acatcatact gcttttagaa gggaaaaatt aaaagggaga gactccgctc
     3061 tcccattatt ggctatttcg cagggttact gcgtggtacc gtcggttttg gtatcgacat
     3121 cattgttgat gccatcaccg gtttggactt ttttattgat atccgggcag cgaccatctt
     3181 tgcacatggt gttcttgtgc tcttcatctt tggtcatgcc gtcattgttc attgaagaac
     3241 catccggatg cagcattgtg ccaccagaac cggtatttac cccgttattg tcgacgttat
     3301 ttggcgcgac attttcacgg gcatcagggg ctacctggcc cgcatcagct gcggcgtttg
     3361 cctggccgtt attgctttgc gctccgctat cggcagccag tgcggcaccg ctggcaaggc
     3421 ttagagtggc agtcaaaaat agtgtggcca gttttgtcat tttcatagga tgctcctgtt
     3481 atggtcgtta tgtcggataa cctcttccaa cagtgcattt gcaggtgaat ataggcaatg
     3541 gtttaagatt tcagccaggc taagataccc agcagagaat cttgaaataa ttaacaaaca
     3601 aaggagttac agttagaaat tgtaggagag atctcgtttt tcgcgacaat ctggcgtttt
     3661 tcttgctaat tccaggatta atccgttcat agtgtaaaac cccgtttaca cattctgacg
     3721 gaagatatag attggaagta ttgcattcac taagataagt atggcaacac tggaacagac
     3781 atgaattatc agaacgacga tttacgcatc aaagaaatca aagagttact tcctcctgtc
     3841 gcattgctgg aaaaattccc cgctactgaa aatgccgcga atacggtcgc ccatgcccga
     3901 aaagcgatcc ataaaatcct gaaaggtaat gatgatcgcc tgttggtggt gattggtccg
     3961 tgctcaattc atgatcctgt cgcggctaaa gagtatgccg ctcgcttgct gacgctgcgt
     4021 gaagagctgc aagatgagct ggaaatcgtg atgcgcgtct attttgaaaa gccgcgtact
     4081 acggtgggct ggaaagggct gattaacgat ccgcatatgg ataacagctt ccagatcaac
     4141 gacggtctgc gtattgcccg caaattgctg ctcgatatta acgacagcgg tctgccagcg
     4201 gcgggtgaat tcctggatat gatcacccca caatatctcg ctgacctgat gagctggggc
     4261 gcaattggcg cacgtactac ggaatcgcag gtgcaccgcg aactggcatc agggctttct
     4321 tgcccggtag gcttcaaaaa tggcactgat ggtacgatta aagtggctat cgatgccatt
     4381 aatgccgccg gtgcgccgca ctgcttcctg tccgtaacga aatgggggca ttcggcgatt
     4441 gtgaatacca gcggtaacgg cgattgccat atcattctgc gcggcggtaa agagcctaac
     4501 tacagtgcga agcacgttgc tgaagtgaaa gaagggctga acaaagcagg cctgccagca
     4561 caggtgatga tcgatttcag ccatgctaat tcgtccaaac aattcaaaaa gcagatggat
     4621 gtttgtgctg acgtttgcca gcagattgcc ggtggcgaaa aggccattat tggcgtgatg
     4681 gtggaaagcc atctggtgga aggcaatcag agcctcgaga gtggggaacc gctggcctat
     4741 ggcaagagca tcaccgatgc ctgcattggc tgggatgata ccgatgctct gttacgtcaa
     4801 ctggcgaatg cagtaaaagc gcgtcgcggg taaggtttaa ttgtcggatg cgccgtcaga
     4861 gtggcgtatc cgatgaatca ccgtagccct gacaagacgc gcagcgtcgc atcaggcaat
     4921 gtgctccatt gttagcaacg acaaacaaaa aagccgactc acttgcagtc ggctttctca
     4981 tttttaagcg aatgacgttt acttcgcttt accctggttc gcaacggccg ctgctttcgc
     5041 cgcgatctcg tcagcattac ccagataata gcgtttcaac ggtttgaaat tctcgtcgaa
     5101 ctcatacacc agtggcacac cagtcgggat attcagctca agaatctctt cttcgctcat
     5161 gttatcgaga tatttcacca gcgcacgtaa cgagttaccg tgtgcggcga tgattacgcg
     5221 ctcaccgctc ttcatacgcg gcagaatggt ttcattccag taagggatca cgcggtcaat
     5281 ggtcagcgcc aggctttccg tcagtggcag ttctttctcg ctcagcttcg cgtaacgtgg
     5341 atcgtgaccc ggataacgct catcatcttt agtcagttcc ggcggagtca ctgcaaaacc
     5401 acgacgccac tgtttcacct gctcgtcgcc atacttttca gcggtttctg ctttgttcag
     5461 accctgcaac gcaccatagt gacgttcgtt cagtttccag gatttctcaa cgggcagcca
     5521 tgcctgatcc agttcgtcca gcacattcca cagggtatgg atagcgcgtt tcagcacaga
     5581 ggtgtaagca aagtcaaagc tgtaaccttc ctctttcagc agcttacctg ctgcttttgc
     5641 ttcgcttacg cctttctcag acagatccac gtcgtaccaa ccggtgaaac ggttttcttt
     5701 gttccactga ctttcgccat gacgaaccag aaccagctta gttacagcca tatacttact
     5761 cctcaaatca tcttttaatg ataataattc tcattatatt gccgtgacga agcaacagca
     5821 atgcttacgc ataaccatag cgaaaatagt gtcgcagtgt aaggttgttg tgaatattga
     5881 gttgcaaata tgtcggtgtt cgctggtgat ttgaacaata tgaagataaa gccctcatga
     5941 cgagggctta acattactca gcaataaact gatattccgt caggctggaa tactcttcgc
     6001 caggacgcag gaagcagtcc ggttgcggcc attcagggtg gttcgggctg tccggcagaa
     6061 actcgctttc cagagccagc ccatgccagt cggcgtaagg ttcggttccc cgcgacggtg
     6121 tgccgccgag gaagttgccg gagtagaatt gcagagccgg agcggtggtg tagaccttca
     6181 gctgcaattt ttcatctgct gaccagacat gcgccgccac tttcttgcca tcgcctttgg
     6241 cctgtaacaa gaatgcgtga tcgtaacctt tcactttgcg ctgatcgtcg tcggcaagaa
     6301 actcactggc gatgattttg gcgctgcgga aatcaaaaga cgttccggcg acagatttca
     6361 ggccgtcgag cggaatgccg ccttcatcaa ccggcagata ttcgtccgcc agaatctgca
     6421 acttgtgatt gcgcacgtca gactgctcgc cgtcaagatt gaaatagacg tgattagtca
     6481 tattcaccgg gcaaggttta tcaactgtgg cgcgataagt aatggagata cggttatcgt
     6541 cggtcagacg atattgcacc gtcgcaccga gattacccgg gaagccctga tcgccatcat
     6601 ctgaactcag ggcaaacagt acctgacgat cgttctggtt cacaatctgc cagcgacgtt
     6661 tgtcgaaccc ttccgggccg ccgtgcagct ggttaacgcc ctgacttggc gaaagcgtca
     6721 cggtttcacc gtcaaaggta taacggctat tggcgatacg gttggcataa cgaccaatag
     6781 aggcccccag aaacgcggcc tgatcctgat agcattccgg gctggcacag ccgagcagcg
     6841 cctcgcggac gctgccatcg gaaagcggaa tacgggctga aagtaaagtc gcaccccagt
     6901 ccatcagcgt gactaccatc cctgcgttgt tacgcaaagt taacagtcgg tacggctgac
     6961 catcgggtgc cagtgcggga gtttcgttca gcactgtcct gctccttgtg atggtttaca
     7021 aacgtaaaaa gtctctttaa tacctgtttt tgcttcatat tgttcagcga cagcttgctg
     7081 tacggcaggc accagctctt ccgggatcag cgcgacgata cagccgccaa atccgccgcc
     7141 ggtcatgcgt acgccacctt tgtcaccaat cacagctttg acgatttcta ccagagtgtc
     7201 aatttgcggc acggtgattt cgaaatcatc gcgcatagag gcatgagact ccgccatcaa
     7261 ctcgcccata cgtttcaggt cgccttgctc cagcgcgctg gcagcttcaa cggtgcgggc
     7321 gttttcagtc aggatatgac gcacgcgttt cgccacgatt gggtccagtt catgcgcaac
     7381 agcgttgaac tcttcaatgg tgacatcgcg cagggctggc tgctggaaga aacgcgcacc
     7441 ggtttcgcac tgttcacgac gggtgttgta ttcgctgcca accagggtac gtttgaagtt
     7501 actgttgatg atgacgacag ccacaccttt gggcatggaa actgctttgg tccccagtga
     7561 gcggcagtca atcagcaagg catgatcttt cttgccgagt gctgaaatta gctgatccat
     7621 gatcccgcag ttacagccaa caaactggtt ttctgcttcc tgaccgttaa gcgcgatttg
     7681 tgcgccgtcc agcggcagat gataaagctg ctgcaatacg gttccgaccg cgacttccag
     7741 tgaagcggaa gaacttaacc cagcaccctg cggcacattg ccgctgatca ccatgtccac
     7801 accgccgaag ctgttgttac gcagttgcag atgtttcacc acgccacgaa cgtagttcgc
     7861 ccattgatag ttttcatgcg cgacaatggg cgcatcgagg gaaaactcgt cgagctgatt
     7921 ttcataatcg gctgccatca cgcgaacttt acggtcatcg cgtggtgcac aactgatcac
     7981 ggtttgataa tcaatcgcgc agggcagaac gaaaccgtcg ttgtagtcgg tgtgttcacc
     8041 aatcaaattc acgcggccag gcgcctgaat ggtgtgagtg gcagggtagc caaatgcgtt
     8101 ggcaaacaga gattgtgttt tttctttcag actcatttct tacactccgg attcgcgaaa
     8161 atggatatcg ctgactgcgc gcaaacgctc tgctgcctgt tctgcggtca ggtctcgctg
     8221 ggtctctgcc agcatttcat aaccaaccat aaatttacgt acggtggcgg agcgcaacag
     8281 aggcggataa aagtgcgcgt gcagctgcca gtgttgattc tcttcgccat taaacggtgc
     8341 gccgtgccag cccatagagt aggggaagga gcactggaag aggttgtcat aacgactggt
     8401 cagctttttc aacgccagcg ccaaatcgct gcgctggccg tcggtcaaat cggtgatccg
     8461 caaaacgtgg gctttgggca gtagtagcgt ttcgaacggc caggcagccc agtaaggcac
     8521 tacggctaac cagtgttcgg tttcgacaac ggtacggcta ccgtctgcca gctcgcgctg
     8581 aacataatcc accagcattg gtgatttctg ttcggcaaaa tattcttttt gcaggcggtc
     8641 ttcacgctca gcttcgttag gcaggaagct atttgcccaa atctgtccgt gcggatgcgg
     8701 gttagagcag cccatcgccg cacctttgtt ttcaaagacc tgcacccatg ggtatgtttt
     8761 ccccagttct gcggtttgct cctgccaggt tttgacgatt tccgtcaatg ccgcaacgct
     8821 cagttctggc aacgttttac tgtgatccgg tgaaaagcag atcacccggc tggtaccgcg
     8881 cgcgctctgg caacgcatca gcggatcttt gctttctggc gcatctggcg tgtcagacat
     8941 caaggccgca aagtcattag tgaaaacgta agtcccggtg taatcggggt ttttatcgcc
     9001 tgtcacccgc acattacctg cgcagaggaa gcaatctgga tcatgcgcag gtaacacctg
     9061 tttggctggc gtttcctgcg ccccctgcca ggggcgctta gcgcggtgcg gtgaaaccag
     9121 aatccattgc ccggtgagcg ggttgtagcg gcgatgtgga tgatcaacgg gattaaattg
     9181 cgtcatggtc gttccttaat cgggatatcc ctgtggatgg cgtgactgcc agtgccaggt
     9241 gtcctgcgcc atttcatcga gtgtgcgcgt cacgcgccag ttcagttcac ggtcggcttt
     9301 gctggcgtcc gcccagtagg ccggaaggtc gccctcgcga cgcggtgcaa aatgatagtt
     9361 aaccggtttg ccgcaggctt tgctgaaggc attaaccacg tccagcacgc tgctgcctac
     9421 gccagcgccg aggttgtaga tgtgtacgcc tggcttgttc gccagtttct ccatcgccac
     9481 gacgtgaccg tccgccagat ccattacgtg gatgtaatcg cgtacgccag taccgtcttc
     9541 ggtcggataa tcgttaccaa aaatcgccag cgagtcgcga cggcctacag caacctgggc
     9601 gatgtatggc atcaggttat tcggaatgcc ttgcggatct tcgcccatat cgcccgacgg
     9661 atgtgcgcca accgggttga agtagcgcag cagggcaatg ctccagtccg gctgggcttt
     9721 ttgcagatcg gtgaggatct gttcaaccat cagtttgctt ttgccgtaag ggctttgcgg
     9781 tgtgccggtc gggaagcttt caacgtatgg aattttgggc tgatcgccat aaacggtggc
     9841 agaggagcta aaaataaagt ttttgacgtt cgcggcgcgc atggcgctaa tcaggcggag
     9901 agtaccgttg acattgttgt cgtaatattc cagcggtttt tgtaccgatt cgccaacggc
     9961 tttcagcccg gcgaagtgga tcacggtgtc gatagcgtga tcgtgcagga tctcggtcat
    10021 caacgcttcg ttacgaatat cgccttcaac aaacgttgga tgtttgccgc ctaaacgctc
    10081 gataacgggc agtacgctgc gcttactgtt acagaggtta tcaagaatga tgacatcatg
    10141 accgttttgc agtaattgca cacaggtatg acttccaatg taaccgctac caccggtaac
    10201 cagaactctc ataattcgct ccattaggct tatggtatga aataaccata gcataacaaa
    10261 gatgcgaaaa gtgtgacatg gaataaatta gtggaatcgt ttacacaaga atttagccgt
    10321 tttttatgcg cgattaagtg attataaaac agagggttta tgaatgattg cgctttttat
    10381 ctgaaaaaaa agacgcggtt tcatgcctgg atgcgtcgaa ccgttggccg gagagggcgc
    10441 ttaggccgcc tccggcacgg tcagcactac cgactcaatt tatttttgtc agcacatagc
    10501 gatagagtcc gccgtctggc acgaactcaa gacgatgggt aatacaggca ggcgcatctt
    10561 cagcgtggtg cgaaacaaac aacaattgcg tttcaccttc gctaatcagc acatcaacaa
    10621 accggcggat aagctggcga ttgagcggat caagcccctg tagtggttca tcgagaataa
    10681 gcaacgtcgg atgtttcacc agcgcgcgga cgatcagcgc caggcgttgt tgcccccagg
    10741 aaagactatg gaacggagcg tcagccgtgc gtttatcaat gccgagaata tccagccact
    10801 gctgcaccag tttttgctgg cgatccgaaa cggcctgata aatgccaatc gaatcaaaat
    10861 agccagaaag aatcacatta cgcacagtag tactgacccg gtaatccaga tgcaaactac
    10921 tgctgacgta accgatatgc tttttgatat cccagatggt ttcgccgctg ccgcgacgtc
    10981 gtccgaaaag cgtcaaatcg ttgctgtaac cttgcggatg atcgccagta atcaggctta
    11041 ataacgtcga ttttcccgcg ccatttggcc cgacaatttg ccagtgttca cctggattca
    11101 cctgccagct aaggttatta agaatggggc gatcattata agaaaccacg ccgttgttca
    11161 gcacaatgcg cggttcgttg gcgggtaagg cgtgacgtgc tgaaggttca tccggctccg
    11221 gcagttgtac accttcaagc tgttcgctat gcgccagttg cgcaacgagt gcttgttgga
    11281 gcagttcctc tttagcgccc gtttccgcta acgtacaatc cgccagcacg ccagcaaact
    11341 ggacaaattc cgggatctca tcgaagcgat tgagcaccag taccagagta ataccggact
    11401 gatgtaacga ggcgagtagc tcagccagct gctgacgtga ggcaacatcc aggccatcga
    11461 acggctcatc aagaatcaac aagtcaggct ccgacatcag cgcctgacac agcagggttt
    11521 ttcgcgtctc gccagtggaa aggtatttaa agcgtcggtc gaggagggcg gtaataccga
    11581 actgctgcgc cagttgcatg caacgcggag tatcctttac ttcatcctga atgatctcag
    11641 ccgtggtacg cccggtgtca tcttcgccag gactaagcat atcggtgtta ttccgctgcc
    11701 attcgtcgct gacgagcttt tgcaattgct cgaaggagag acgagtgatg tgggaaaact
    11761 ggctttgccg ttcacctttc aaaagcggaa gttcccccgc cagcgcgcgg gccagggccg
    11821 atttcccgct tccattcgaa ccgacaaacg cccaactatc acccgcgttt aacgttagct
    11881 gaggcaattg cagcgttttt gtgtcgctaa gacgaaatgt gccttgcaaa atttgcaacg
    11941 atgacatttt atatcccatt ttgtgcagcg attactgaca gggatacgtg tttcataaca
    12001 aattgtcaac acgcttagca tagcgtggcg ataatcacgc tgtcggcatt aaagtaggcc
    12061 gtgacattct gtccttgctg aagagaagtc gcttcattta ccggcactgt ggcgcacagt
    12121 gtttgcccgt cgggtagcgc cattaatact tcgcactgct ctgcgccgcg ctcaatatga
    12181 ctaataatac ccggtaattg gttgtcggcg ttttgtgcga ccgcttcgtc ctgagtaatg
    12241 cctacccacg gcgcttttag taatatcaac acttctttgc cttcatccag ccccagacgc
    12301 gcgccgcttt gtgcggtaat tgcgactttc aggcgtgttt ttccgtcagc cagtaagaca
    12361 tcaacatgct gttgaacgtc atcatgatcg cgggcggtga tggtgccaaa ccattggtta
    12421 cgggcgctgg tttgcagtga aaaacgtgag atcgcggcca gcaggctgtt cagcggcagg
    12481 gcgtcatcgt cacttaacac atcaaaggct ttttgctgga tttgcgccag taagtcatag
    12541 agctgaatca gtcgctgacc atagcgggtc agtactgcgc cgccgccacc tttaccgcct
    12601 gttgcgcgct cgaccagaat atgctcactt aactgattca tctcgttaat ggcatcccag
    12661 gcacttttat agctgatacc ggcatctttc gctccctggc taatggaacc ggaaagcgca
    12721 atgtgtttta gtagcgaaat gcggcgcggg tcggcgaata atttttgttg gagcttaagg
    12781 gtgagaagga tttcggcctg cataacaatg tcctggcaaa agtcttattg tgacggaaaa
    12841 cgaacgccac gcaaagctga ccgcacaaaa ggggagtgct tttctgtgct tagcggttag
    12901 aatagtctca tgactatatc tggagttgac catgttagag ttattaaaaa gtctggtatt
    12961 cgccgtaatc atggtacctg tcgtgatggc catcatcctg ggtctgattt acggtcttgg
    13021 tgaagtattc aacatctttt ctggtgttgg taaaaaagac cagcccggac aaaatcattg
    13081 attccctgaa tgcccgctta gtcgggcatt tcttttgctc aacttcctgc ttttcctgcc
    13141 gatatttttt ctcatctacc tcacaaaggt tagcaataac tgctgggaaa attctgagtt
    13201 agccgttata ttgacgccta cataacgtta cattaagggg ttaccaatgg ctcgtaaatg
    13261 gttgaacttg tttgccgggg cggcactctc tttcgctgtt gctggcaatg cactggcaga
    13321 tgaagggaaa atcacggtgt tcgccgccgc atcactgact aacgcaatgc agaacattgc
    13381 tacgcagtat aaaaaagaga aaggcgtgga tgtggtttct tctttcgctt cgtcatctac
    13441 tttggcccgt cagattgaag cgggtgcgcc tgcggatctg tttatttctg ccgatcagaa
    13501 atggatggat tatgcggttg ataaaaaagc gatcgataca gctacacgtc agacactgct
    13561 cggcaatagc ctggtcgttg tagcaccgaa agccagcgag cagaaagatt tcaccatcga
    13621 cagcaaaact aactggactt cgctgctgaa tggcggtcgc ctcgcggttg gcgatccgga
    13681 acatgtccct gctggcattt atgcaaaaga agcactgcaa aaactgggcg catgggatac
    13741 gctctccccg aaactggctc cggcggaaga tgttcgtggc gcgctggcgt tggtcgaacg
    13801 caatgaagcg ccgctgggta ttgtttacgg ttctgacgca gttgccagca aaggggtaaa
    13861 agtggttgcc accttcccgg aagattcaca taaaaaagtg gaatatccgg ttgctgttgt
    13921 ggaagggcat aataatgcga cagtgatagc attttatgat tatctgaagg ggccgcaggc
    13981 tgcggaaatc tttaaacgtt acggatttac aaccaagtaa tgatactgac cgatccagaa
    14041 tggcaggcag ttttattaag cctgaaagtt tcttccctgg ctgtgctgtt tagcctgccg
    14101 tttgggatct tttttgcctg gttactggta cgttgcacgt ttccgggcaa agctctgctc
    14161 gatagcgtac tgcatctacc gctggtgtta ccgcccgtgg tcgtcggtta cttattatta
    14221 gtttcgatgg gacggcgcgg atttatcggt gaacgtctgt atgactggtt tggtctttct
    14281 ttcgcgttta gctggcgcgg tgcggttctc gctgccgctg tcatgtcttt tccgctgatg
    14341 gtgcgggcga ttcgtctggc gctggaaggt gttgatatca aactggagca ggccgcaaga
    14401 acactggggg ccgggcgctg gcgagttttc tttactatca cgttaccgct gaccttaccg
    14461 ggcattattg ttggtacggt actggctttt gctcgttctc tcggtgagtt tggtgcaacc
    14521 atcacctttg tgtcgaacat tcctggcgaa acgcgaacta ttccctctgc catgtatacc
    14581 ctgatccaga cccccggcgg ggaaagtggc gcggcgagac tgtgcattat ttctattgcg
    14641 ctggcgatga tctccctctt gatttcagaa tggctggcca gaatcagccg tgaacgggcg
    14701 gggcgctaat catgctggaa ctgaattttt cccagacgtt gggcaaccat tgcctgacca
    14761 ttaatgaaac gctgcccgcc aatggcatca ctgctctctt tggcgtctcc ggtgccggaa
    14821 aaacttcgct gattaacgcc atcagtggac tgacgcgccc gcaaaaaggg cggattgtcc
    14881 tcaatgggcg ggtactaaat gatgccgaaa aaggtatctg cctgacgccg gaaaagcgtc
    14941 gcgttggcta tgtttttcag gatgcgcggc tgttcccgca ttacaaagtg cgtggcaatc
    15001 tgcgctacgg catgtcgaaa agtatggtcg atcagttcga taagctggtg gcgcttttag
    15061 gcattgaacc gttgcttgac cgtttaccag gcagcctgtc cggaggcgaa aaacagcgcg
    15121 tggcgattgg tcgggctttg ctgacagcac cggaattgct gttgctggat gaaccgctgg
    15181 cgtcactgga tattccgcgt aaacgcgaac tgttgcctta tctgcaacgg ctgacacggg
    15241 aaatcaacat tccgatgttg tatgtcagcc attcgctgga tgagatcctc catctggcag
    15301 acagagtgat ggtactggaa aacggtcagg tgaaagcctt tggcgcgctg gaggaagtgt
    15361 ggggcagtag cgtgatgaat ccgtggctgc cgaaagagca acaaagtagc attctgaaag
    15421 tgacggtgct tgagcatcat ccgcattacg cgatgaccgc gctggcgctg ggcgatcagc
    15481 atttgtgggt caataagctg gacgaaccgc tgcaagctgc gctacgtatc cgcattcagg
    15541 cttccgatgt ttctctggtt ttacaaccgc cgcagcaaac cagcattcgt aacgtattgc
    15601 gggcaaaagt tgttaatagt tatgacgaca acggccaggt ggaagtggag ctggaagtcg
    15661 gcggtaaaac gctgtgggcg cgtatcagcc cgtgggccag ggatgaactg gcgatcaaac
    15721 caggcctgtg gctgtacgcg caaattaaaa gtgtgtcgat aaccgcctga ttaaatcagg
    15781 tggctataaa tgaaccgggc aatgctgtcg gtggtgttat caccaatcac aatgttggcg
    15841 cgcgctttta ccgcatcatc ggcgttgccc atcgccacgc ctgtaccagc ggcctccagc
    15901 atactgatat cattaaagtt atcgccgaag gccacgacgt tttccatcga ccagccttgc
    15961 gcctcaaccc atttcgtcaa acgtttacct ttgctgttgc cgccgcgtgc aatatcaacc
    16021 tgatcctgcc aggaccattc acactcaagt cccagttcat gttcgacatg cttaccaaaa
    16081 tgctgcaatt gcggcaggtc atcgtgcgtc agggcgaact tccatacggc gttaacttgt
    16141 tgcgccgttt cagccagaga agcgacttgt gtgaaagtcg gacgctgttc cggcggcagg
    16201 gtttgcgccc agttagacgt gcgaatgaca tgcccggtcg ggtgctcata gaccatcgca
    16261 tcatccacat acatcagacc gtgaatgtgg tgttcattca gcatctcaat gagttgcagg
    16321 gctttattaa cgggcattgg gtccgcttcc agcactgttt ttgcatgata atcatacaaa
    16381 taggtgccat tacagcaaat agcaggtgta tccagcgcca gcgcctgata aaaaggatga
    16441 atagcgacgt gatggcgacc tgtgacgatg attaattgat agcctgcttc gcaagcgcgg
    16501 gctagggctt ctatcgatga tggaagcaag gtctttttcg gggtcaataa ggtgccgtct
    16561 aagtcgagag caatcacgcg tgtggtcatg gcgtattcca gattaaggtt aagaattttc
    16621 gtctgcgcga atggtacacc gataccacta tcggacaaaa ttctgcgttt taattcagca
    16681 ttcaccgcca aaagcgacta attttagctg ttacagtcag ttgctaaatg caaaggagca
    16741 ttcatgaagc aaacagttta tatcgccagc cctgagagcc agcaaattca cgtctggaac
    16801 ctgaatcatg aaggcgcact gacgctgaca caggttgtcg atgtgccggg gcaggtgcag
    16861 ccgatggtgg tcagcccgga caaacgttat ctctatgttg gtgtgcgccc tgagtttcgc
    16921 gtcctggcgt atcgtatcgc cccggacgat ggcgcactga cctttgccgc agagtctgcg
    16981 ctgccgggta gtccgacgca tatttccacc gatcaccagg ggcagtttgt ctttgtaggt
    17041 tcttacaatg cgggtaacgt gagcgtaacg cgtctggaag atggcctgcc agtgggcgtc
    17101 gtcgatgtgg tcgaggggct ggacggttgc cattccgcca atatctcacc ggacaaccgt
    17161 acgctgtggg ttccggcatt aaagcaggat cgcatttgtc tgtttacggt cagcgatgat
    17221 ggtcatctgg tggcacagga ccctgcggaa gtgactaccg ttgaaggggc cggcccgcgt
    17281 catatggtat tccatccaaa cgaacaatat gcgtattgcg tcaatgagtt aaacagctca
    17341 gtggatgtct gggaactgaa agatccgcat ggcaatatcg aatgtgtcca gacgctggat
    17401 atgatgccgg aaaacttctc cgacacccgt tgggcggctg atattcatat caccccggat
    17461 ggtcgtcatt tatacgcctg cgaccgtacc gccagtctga taactgtttt cagcgtttcg
    17521 gaagatggca gcgtgttgag taaagaaggc ttccagccaa cggaaaccca gccgcgcggc
    17581 ttcaatgttg atcacagcgg taagtatctg attgccgccg ggcaaaaatc tcaccacatc
    17641 tcggtatacg aaattgttgg cgagcagggg ctactgcatg aaaaaggccg ctatgcggtc
    17701 gggcagggac caatgtgggt ggtggttaac gcacactaac cgctgattta cccggcgcag
    17761 tctcttctgc gccggtgtat taacctatct cctgtaacgc atgtctctgg cgttcgacga
    17821 tattggtcca caaattgtct ttatcgtcag tccacaaatt aatcagcaag gcaaaaaagc
    17881 gttcagctgc cggagaaagc acggcatctt tgcggcgaat aatccccaat gtccgacgta
    17941 taaccggttc caccagcggg ataccaataa tcgaagagta gggcgcatga ggcattgcca
    18001 ggcctggaag cgccgaaatc cctagtcctg cctccaccag tcctaatgac gtcgaaagat
    18061 gacgcacttc gtaaaaccag tccagcttcc agggcttgtc ggctagctgt tgttctatca
    18121 acagtcggtt gccgctggag gagcgtacgc caatcatttt gtaacccact aactcctgcc
    18181 attctacaag ctgctttttg gccaatggat ggtcacgccg acaggccagc acgaacggct
    18241 cgttgaccag gggggtaaaa tcaatggatg aatttgtgac gttgttcatg tttatgccaa
    18301 agtctgattc gttacacagc accgattcca tgcaattatt cgtgccttgt tccagaatcc
    18361 gcactttaat attgggatac agctcattaa atttaccgat tgccagcggt aaaaaataaa
    18421 atactgcggt cggaatacac gctaatgtca ccataccacg atgataagca ttcatatcac
    18481 gaatattaaa aagcgtttca tcgaatttct ttattaattc ccgcgcctcc gggagcaacc
    18541 tttttcctgc ttttgttaac gtaaccttgc gagttgtacg ctcaaatagt tgaatatgta
    18601 aatcttcctc catctttttt attcttcgcg ttaatgcagg ttgcgtaata ttgagtaatt
    18661 tagcggcatt gttaaatgaa ctggactctg ccagtatgac aaatgccttc atacttgata
    18721 attcatgctt cattattact ccggaaaatg gaagcgtcga ttttgggtgg ctggccgtta
    18781 aaaattttaa ctgcatttag ccaacttaaa ttaatgaaaa aatgttatta atcgttgagc
    18841 taaagtcatt agagatgctt tgcccttaat gtaaccatat cgcaataagt tatgttttta
    18901 aattgagggc attattatga aaaaaatacc ctgcgtgatg atgcgaggtg gaacctcgag
    18961 gggcgcgttc ctgttagcgg aacatttacc cgaagatcaa acgcagcgcg ataaaatatt
    19021 gatggcaatt atgggttccg gtaacgatct ggaaattgac ggtattggcg gcggtaatcc
    19081 gctgaccagt aaagtcgcca ttattagccg ctccagcgat ccgcgtgcag atgtcgatta
    19141 tctgtttgct caagttatcg tccatgagca acgtgtcgat accacgccta actgcggcaa
    19201 tatgctgtct ggcgttgggg cattcgccat tgaaaatggt ttgattgcag cgacttcgcc
    19261 agttacccgc gtacgtatcc gcaacgtcaa tacgggtacg ttcatcgaag ctgatgtgca
    19321 aacgccaaat ggtgttgtcg agtacgaggg tagcgccaga attgacggcg taccgggtac
    19381 tgccgcaccg gttgcgctca ctttcctgaa tgccgccgga accaaaaccg gaaaagtctt
    19441 cccgacggat aatcagattg attattttga cgatgtcccg gtgacctgta tcgatatggc
    19501 gatgccagtt gtcattattc ctgctgaata tctgggaaaa acaggctatg aattaccggc
    19561 ggagctggat gccgacaaag cattattagc ccgcattgaa tctatccgtc tacaagcggg
    19621 taaagcaatg ggcttaggcg atgtcagtaa tatggttatc cctaaacctg tgcttatttc
    19681 tccagcgcag aaaggcggag caattaatgt gcgttatttt atgccgcatt cttgccatcg
    19741 cgcgctggcg ataaccggtg ctattgccat ttccagtagt tgtgcattgg aaggcaccgt
    19801 cacccgacaa atcgtccctt ctgtaggata cggcaatatc aatattgaac accccagtgg
    19861 tgcgctcgac gttcatttaa gtaatgaagg tcaggatgcc acgacgttac gcgcatctgt
    19921 tattcggaca accagaaaaa tattttccgg tgaagttttt cttccctgaa aaaattcgtt
    19981 gtcaggataa ggacaatcaa taaaggactt ctgtatgagt catacagaaa gaacaggatt
    20041 ttaaatgaat aaaaaatcgt tatggaagct aattctgata ttagcgatcc catgtattat
    20101 tggctttatg ccagctccgg caggattaag cgaactggcg tgggtgcttt ttggtattta
    20161 cctggcggcc attgtggggc tggttatcaa gcctttcccg gaacctgtcg tactgttaat
    20221 tgccgttgct gcctccatgg tggtggtcgg taacttatcc gacggtgcgt ttaaaaccac
    20281 cgccgtatta agcggttact cttcaggtac cacctggctg gtgttctcgg cgtttacctt
    20341 aagcgccgca tttgtgacca ccggcttagg taaacgtatt gcctatttgc tgattggtaa
    20401 aattggtagc actaccctgg gtctgggtta cgttacggta tttctcgatc tggtactggc
    20461 tccggcaaca ccgtctaaca ccgcgcgtgc gggcggcatt gtgttaccga tcatcaacag
    20521 cgtggcggtg gctttggggt ccgaaccgga aaaaagcccg cgtcgtgtcg gacattacct
    20581 gatgatgtcc atttacatgg tcaccaaaac caccagctat atgttcttta ccgcaatggc
    20641 ggggaacatt ctggcgctga aaatgatcaa cgatattctg cacctgcaaa ttagctgggg
    20701 tggatgggcg ctggccgccg gattgccggg tatcattatg ctgctggtca ccccgctggt
    20761 gatttacacc atgtatccgc cagaaattaa gaaggtggat aacaaaacca tcgccaaagc
    20821 gggccttgcc gaactgggac cgatgaaaat ccgcgaaaaa atgctgctcg gtgtcttcgt
    20881 gctggcgctg ctgggctgga ttttcagtaa gtcactgggg gttgatgaat ccaccgtggc
    20941 aatcgttgtt atggcgacca tgctgctgct gggtatcgtt acctgggaag acgtggttaa
    21001 aaataaaggc ggctggaata ccttaatctg gtacggcggt attatcggct taagctcctt
    21061 attatcgaaa gttaaattct tcgaatggtt agctgaagtc tttaaaaata acctggcatt
    21121 tgatggtcac ggtaacgttg ctttcttcgt tattattttc ctcagcatta tcgtgcgtta
    21181 tttcttcgct tccggtagtg cctatatcgt tgccatgtta ccggtatttg ccatgctggc
    21241 gaacgtctcc ggcgcaccgt taatgttaac cgcgctggca ctgttgttct ccaactccta
    21301 tggcggcatg gttactcact atggcggcgc ggcaggtccg gtcatctttg gcgtgggtta
    21361 taacgatatt aaatcctggt ggttggtcgg tgcggtactg acgatattaa ccttcctggt
    21421 gcatatcacc cttggcgtgt ggtggtggaa tatgctgatc ggctggaaca tgctgtaaat
    21481 atactcgtca tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc cagtcactta
    21541 cttatgtaag ctcctgggga ttcactcgat tgtcgccttc ctgcaactcg aattatttag
    21601 agtatatcca tttattatct ttctgcgcac ttctcggtgc gcagatatct ggagcatttg
    21661 atgatcaagt tatctgaaaa aggcgtgttt ctcgccagta ataacgaaat aattgccgaa
    21721 gaacatttca ccggcgaaat taaaaaagaa gaagcaaaaa aaggcactat tgcctggtct
    21781 attctctctt ctcataatac gtccggaaat atggataaac ttaaaattaa gtttgattca
    21841 ttagcctctc acgatattac ctttgttggt attgtacaga ccgctaaagc gtccggtatg
    21901 gaacgtttcc cgctgccgta tgtgctgacc aactgccata actcactttg cgccgtcggc
    21961 ggcaccatta acggtgatga ccatgttttt ggtttatcgg cggctcagcg ttatggcggt
    22021 atttttgtgc ctccgcatat tgcggtcatc catcaatata tgcgcgagat gatggcaggc
    22081 ggcggcaaaa tgatcctcgg gtcagacagc cacacccgtt acggtgcatt agggacaatg
    22141 gcagtcggtg agggcggcgg cgagttggta aaacaattgc ttaatgacac ctgggatatc
    22201 gactatccgg gagttgttgc ggtgcatctg accggaaaac cagcgccgta tgtggggcca
    22261 caggatgtgg cgctggctat catcggtgcc gtgttcaaaa acggctacgt caaaaacaaa
    22321 gtgatggaat tcgtaggtcc cggtgttgct gcgctctcta ccgatttccg taacagcgtt
    22381 gacgttatga ccacggaaac gacctgttta agttctgtct ggcaaaccga tgaagaagtc
    22441 cataactggc tggcgctgca cggtcgcggc caggattact gccagcttaa ccctcaaccg
    22501 atggcgtact acgatggctg catcagcgtt gatttaagcg ccatcaaacc aatgattgcg
    22561 ctgccgttcc acccgagcaa cgtgtatgaa atcgacatgc tgaaccagaa cctgaccgac
    22621 attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc acggtaaagc caaactctcg
    22681 ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc aggggattat cgcaggctgt
    22741 tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg cactgcgcgg tcaatcctgt
    22801 ggcaatgaca ccttctcgtt agcggtttac ccgtcatcac agccggtgtt tatggattta
    22861 gccaaaaaag gcgtggtagc agatttgatt ggcgcaggcg caatcatcag aaccgcgttc
    22921 tgcggcccat gctttggcgc gggcgatacg ccaatcaaca acggtttgag cattcgccac
    22981 accacgcgta acttcccgaa ccgcgaaggc tctaagccag ccaatgggca gatgtcagcg
    23041 gtggcgttga tggacgctcg ttctatcgct gcgactgcgg caaacggtgg ctatttaacc
    23101 tctgccagcg aacttgattg ctgggacaac gtgccggagt acgccttcga tgtaacgccg
    23161 tataaaaacc gtgtttatca gggctttgtg aaaggggcga cccagcaacc gctgatttac
    23221 ggaccgaaca ttaaagactg gccggaattg ggtgcgctga ctgacaatat cgtcctcaaa
    23281 gtgtgctcga agatcctcga cgaagtcacc accaccgacg aactgattcc ttccggtgaa
    23341 acctcttctt atcgttcaaa tccgattggt ctggcggagt ttaccctgtc acgccgcgat
    23401 ccaggttatg ttggcagaag taaagcgact gctgagctgg aaaatcagcg tctggcgggg
    23461 aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc agattactgg tcaggagcat
    23521 attgatccgc tgcaaactga aattggcagc atggtctatg cggtgaaacc aggcgatggt
    23581 tctgcgcgtg agcaggcggc gagctgccag cgtgtgattg gcggtctggc gaatattgcc
    23641 gaggagtacg cgactaaacg ctaccgttct aacgtcatca actgggggat gttaccgctg
    23701 cagatggcgg aagcgccaac gtttgaagtg ggggattaca tttacatccc tggcattaaa
    23761 gcagcgctgg ataatccggg tacgacgttt aaaggttatg tgatccatga agatgcgccg
    23821 gtaacggaaa ttacgcttta catggaaagc ctgacggccg aagagcgcga gattatcaag
    23881 gcgggtagtt tgattaactt caataaaaac cgtcagatgt aaaaaaaggc gctatgaaat
    23941 atagcgccca gtttcattaa cccgatggtt cagactatcg ggttttcttt tttacttctt
    24001 cgcctctgca accactttgc tacccacgcc gcggttattg tattcccaca tgcggttgta
    24061 gttagtgtca ttcatattgc gttgtatttc gtcgctatcg tcaacactgc cggtgttacc
    24121 tgcgaatgga cgattggaaa tcaccgcatc ggcccacggt ttcgccgtgt taaaaccttc
    24181 gttgatggcg ctatcacgga tcaccacctg accgttggta ttggcatcaa catccagcga
    24241 gcggcccagt tgcgccacgc catcaccgga agcattgaaa cggctgttta cggcgaggaa
    24301 accgtagtaa atgttggaca gcgtagccgg tgcaaacaca tacgcttctt gctgggtacg
    24361 ggagttcacc acgcggaatt cggtgttatc gaacaccact gcgccgcgac cagaaacgat
    24421 atccacatcc ccttcaatat agctgttggt caccagcgta cgcggctgac gattcgtttc
    24481 cagacggttc tgcacaccgc tattggtgac aaagaaggtg ttctgacgac cgagaatgtt
    24541 aacgttgtta atctgtacct ggtcaccatc agtacgcagt gccaccgccg gatggttacc
    24601 cgcatctacg ctatcaccca gcgtgttttc gatggtcagg ttttgcagtt gcaggccatt
    24661 gttttgtgac cagaagaccg cagagcagag aacaccgata ctgtcgctgc gtttgctctg
    24721 gcagctatcg tacatatacc acgctggttt acctggcata tatttgccgc gcgggttgac
    24781 gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg gaaagcccaa tcttcacatc
    24841 aatcggtttt tcacctgtac cgtacagagt aattccaccc ggagcggcag ggacatatac
    24901 cgttccctga tactcaccag gcatcacggc aatatactgg cgcttgttgg tacgcttgat
    24961 aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt acaccttgag tacccgccgg
    25021 gccgacaaca aagtcaggtt gcgcaggcaa ggtaattggg gaaggattcc acgctgccgc
    25081 acctggtgtc agggatgcaa aatagtgttg agcatcgaaa ttctgcgctt cttttgccga
    25141 cagaatcggg cgtgaagagg taccaggcgc ggtttgatca gaaggacgtt gatcgggcgg
    25201 tgttgagcta caggcggtca gcgtcacgcc aaaagccaat gccagcgcca gacgggaaac
    25261 tgaaaatgtg ttcacaggtt gctccgggct atgaaataga aaaatgaatc cgttgaagcc
    25321 tgctttttta tactaacttg agcgaaacgg gaaggtaaaa agacaaaaag ttgtttttaa
    25381 tacctttaag tgataccaga tggcatagcg ccatctggca gagtgattaa ctaaacatcg
    25441 cagtaatcga ggcgcttgcc agagagtgga aatggacgtt aaacccgacc atcgcgccgc
    25501 tggcaccttc atcgacatca atacgttcta catccagcgc gtgaacggta aaaatgtagc
    25561 gatgggtttc gcctttcggc ggcgctgcac catcgtaccc ggttttacca aagtcggtac
    25621 gcgtctgcaa aacgccgtct ggcattgcta ccagaccaga gccaaaccct tgcggtaata
    25681 cgcgggtatc agcgggtaaa ttaacaacga cccagtgcca ccagccggag ccggttggcg
    25741 catccgggtc gtagcaggtg acgacaaaac ttttcgttcc cgcaggaaca tcatcccacg
    25801 ccagatgcgg tgaaatatta tcgccatcgt aacccatgcc gttaaagaca tgacgatgtg
    25861 gcaacttatc gccatcgcgc agatcattac tgatgagttt catgaaccct cctttctagt
    25921 ttgcagaaag tgcagccaga aaccctcacg ctgacttccc gttattggca aaaaaatgtt
    25981 tcatcctgca ccgcacgatt aaccgctgcg gtcagacgct gcaactgttg cgggagaata
    26041 atatagggcg gcatcaggta aatcagtttg ccaaatggtc gtatccagac accctgttcg
    26101 acaaagaatt tttgcagcgc tgccatattc accggacgag tggtttcgac cacgccaatg
    26161 gcccctagca cgcgcacatc ggcaaccatt ttggcatcac gggctggtgc cagttgctcg
    26221 cgcagttgta cttcaatagc caccacctgc tgctgccagt cgccagattc gagaatcgcc
    26281 aggctggcgt ttgctgccgc gcaggctagc ggattgccca taaaagttgg cccatgcata
    26341 aagcaaccgg cttcgccgtt actgatggtt tccgcaacct cgcgcgtggt gagtgtggcg
    26401 gaaagggtca ttgtaccgcc ggttaaggct ttaccgaggc acaaaatgtc cggcgcgata
    26461 tctgcatgtt cacaggcaaa cagtttgcca gtacgaccaa atccggtggc gatctcgtcg
    26521 gcaatcagca agataccttc gcgatcgcac attttgcgga ttcgttttaa ccattccgga
    26581 tggtagatgc gcatcccgcc tgcgccctgg acaatcggtt caatgatcac cgccgcgatt
    26641 tcatgacgat gcgccgccat caggcgggca aagcccacca tatcgcgctc atcccattca
    26701 ccatccatgc ggctttgcgg ggcgggagca aacaagtttt ctggcagata gcctttccac
    26761 aggctgtgca ttgagttgtc cggatcgcac accgacatcg cgccaaaggt atcgccatga
    26821 taaccattgc ggaaggtcag aaaacgctgg cgcgcttcgc ctttggcctg ccagtactgc
    26881 aacgccattt tcatcgccac ttctaccgct acggagccgg agtccgcgag aaaaacgcat
    26941 tccagcggtt gcggcgtcat cgccaccagt ttgcggcaca gctcaatggc tggcgcatga
    27001 gtgataccgc caaacatcac atgcgacatg gcatcaattt gcgacttcat cgccgcatta
    27061 agctgcgggt gattgtaacc gtggatcgcc gcccaccagg acgacatacc gtcaaccagg
    27121 cgtctgccgt cagacaaaat tagctcgcaa ccttcggcgc tcgccaccgg ataaaccggc
    27181 agcggggagg tcatggatgt gtaggggtgc cagatatggc gttggtcaaa ggcaagatcg
    27241 tccgttgtca taatcgactt gtaaaccaaa ttgaaaagat ttaggtttac gagtctacac
    27301 cgaattaaca acaaaaaaca cgttttggag aagccccatg gctcaccgcc cacgctggac
    27361 attgtcgcaa gtcacagaat tatttgaaaa accgttgctg gatctgctgt ttgaagcgca
    27421 gcaggtgcat cgtcagcatt tcgatccacg tcaggtgcag gtcagcactt tgctgtcaat
    27481 taagaccgga gcttgtccgg aagattgcaa atattgcccg caaagctcgc gctataaaac
    27541 cgggctggaa gccgagcggt tgatggaagt tgaacaggtg ctggagtcgg cgcgaaaagc
    27601 gaaagcggcg ggatcgacac gtttctgtat gggcgcggcg tggaagaatc cccacgaacg
    27661 cgacatgccc tatctggagc agatggtgca gggggtaaaa gcgatgggcc tggaggcgtg
    27721 tatgacgctg ggtacgttga gtgaatctca ggcgcagcgc cttgcgaacg ccgggctgga
    27781 ttactacaac cacaacctcg acacctcgcc ggagttttac ggcaatatca tcaccacacg
    27841 cacttatcag gaacgcctcg atacgctgga aaaagtgcgc gatgccggga tcaaagtctg
    27901 ctcaggtggc attgtgggct taggcgaaac ggtaaaagat cgcgccggat tattactgca
    27961 actggcaaac ctgccgacgc cgccggaaag cgtgccaatc aatatgctgg tgaaggtgaa
    28021 aggcacaccg ctggcggata acgatgatgt cgatgccttt gattttattc gcaccattgc
    28081 cgtagcgcgg atcatgatgc cgacctcata cgtgcgcctt tctgccggac gcgagcagat
    28141 gaatgaacag actcaggcga tgtgctttat ggccggggct aactcaattt tctacggttg
    28201 caaactgctg actacgccga atccggaaga agataaagac ctgcaactgt tccgcaaact
    28261 ggggctaaat ccgcagcaaa ctgccgtgct ggcaggggat aacgaacaac agcagcgtct
    28321 ggagcaggca ctgatgaccc cggacactga cgaatattac aacgcggcag cactatgagc
    28381 tggcaggaga aaatcaacgc ggcgctcgat gcgcggcgtg ctgccgatgc cctgcgtcgc
    28441 cgttatccgg tggcgcaagg ggccggacgc tggctggtgg cggacgatcg ccagtatctg
    28501 aacttttcca gtaacgatta tttaggttta agccatcatc cgcaaattat ccgtgcctgg
    28561 aagcagagtg cggagcaatt tggcgtcggt agcggcggct ccggtcacgt cagcggttat
    28621 agcgtggcac atcaggcgct ggaagaagaa ctggccgagt ggctgggcta ttcgcgggca
    28681 ctgctgttta tctctggttt tgccgctaat caggcggtca ttgccgcgat gatggcgaaa
    28741 gaggaccgta ttgttgccga ccggcttagc catgcctcat tgctggaggc tgccagttta
    28801 agcccgtcgc agcttcgacg ttttacccat aacgatgtcg ctcatctggc gcgactgctt
    28861 gcttccccct gtccggggca gcaactggtg gtgacagaag gcgtgttcag catggacggc
    28921 gatagtgcgc cactggcgga aatccagcaa gtaacgcaac agcacaatgg ctggttgatg
    28981 gtcgatgatg cccacggcac gggcgttatc ggggagcagg ggcgtggcag ctgctggctg
    29041 caaaaggtaa aaccagaatt gctggtggtg acttttggca aaggatttgg cgtcagcggg
    29101 gcagcggtgc tttgctccag tacggtggcg gattatctgc tgcaattcgc ccgccatctt
    29161 atctacagca ccagtatgcc gcccgctcag gcgcaggcat tacgtgcatc gctggcggtc
    29221 attcgcagtg atgagggtga tgcacggcgc gaaaaactgg cggcactcat tacgcgtttt
    29281 cgtgccggag tacaggattt gccgtttacg cttgctgatt catgcagcgc catccagcca
    29341 ttgattgttg gtgataacag ccgtgcgtta caactggcag aaaaactgcg ccagcaaggc
    29401 tgctgggtca cggcgattcg cccgccaacc gtacctgctg gtacagcgcg actgcgctta
    29461 acactaaccg ccgcgcatga aatgcaggat atcgaccgtc tgctggaggt gctgcatggc
    29521 aacggttaat aaacaagcca ttgcagcggc atttggtcgg gcagccgcgc actatgagca
    29581 acacgcagag ttacagcgcc agagtgctga cgtcttactg gcaatgctcc cacagcgtaa
    29641 atacacccac gtactggacg cgggttgtgg acctggctgg atgagccgcc attggagaga
    29701 gcgtcacgcg caggtgacgg cgttagatct ctcgccgcca atgcttgttc aggcacgcca
    29761 taaagatgcc gcagaccatt atctggcggg agatatcgaa tccctgccgt tagcgactgc
    29821 gacgttcgat cttgcatgga gcaatctcgc ggtgcagtgg tgcggtaatt tatccacggc
    29881 acttcgcgag ctgtatcggg tggtgcgccc cggaggcgtg gtcgcgttta ccacgctggt
    29941 gcagggatcg ctccctgaat tacatcaggc gtggcaggcg gtggacgagc gcccgcatgc
    30001 taatcgcttt ttaccgccag atgaaatcga acagtcgctg aacggagtgc attatcaaca
    30061 tcatattcag cccatcacgc tgtggtttga tgatgcgctc agtgctatgc gttcgctgaa
    30121 aggcatcggt gccacgcatc ttcatgaagg gcgctaccca cgaatattaa cgcgttcgca
    30181 gttgcagcga ttgcaactgg catggccgca acaacagggg cgatatcctc tgacgtatca
    30241 tctttttttg ggagtgattg ctcgtgagta aacgttattt tgtcaccgga acggataccg
    30301 aagtggggaa aactgtcgcc agttgtgcac ttttacaagc cgcgaaggca gcaggttatc
    30361 ggacggcagg ttataaaccg gtcgcctctg gcagcgaaaa gaccccggaa ggcttacgta
    30421 atagtgacgc gctggcgtta cagcgcaaca gcagcctgca gctggattac gcaacggtaa
    30481 atccttacac cttcgcagaa cccacttcgc cgcacatcat cagcgcacaa gagggcaggc
    30541 cgatagaatc attggtaatg agcgccggat tacgcgcgct tgaacaacag gctgactggg
    30601 tgttagtgga aggtgctggc ggctggttta cgccgctttc tgacactttc acttttgcag
    30661 attgggtaac acaggaacaa ctgccggtga tactggtagt tggtgtgaaa ctcggctgta
    30721 ttaatcacgc gatgttgacg gcacaggcaa tacaacacgc cgggctgact ctggcgggtt
    30781 gggtggcgaa tgatgtcacg cctccgggaa aacgtcacgc tgaatatatg accacgctca
    30841 cccgcatgat tcccgcgccg ctgctgggag agatcccctg gcttgcagaa aatccagaaa
    30901 atgcggcaac cggaaagtac ataaaccttg ccttgttgta gccatcctgt atttggttaa
    30961 attgcgagcg agatcgcgtc ttcgattgac tgcaatttaa ccaattaaat tctaaaataa
    31021 tcacgaaaaa aattttactt ccgcatcatg cgatgtatgt gggaattgcc caggcggcgg
    31081 gggatagggg ctgcagacag ttatccacta ttcctgtgga taaccatgtg tattagagtt
    31141 agaaaacacg aggcaagcga gagaatacgc ggcttgcacg cgaattggcg ttaaagacgg
    31201 ctcaaagaaa tatcttttat tttttaactg gttagataat tgcaatggca gtcactgaag
    31261 aggcatctct tgccataaaa ctgtcatcac tcatcttgac aaatgttaaa aaagccgttg
    31321 ctttggggat aacccggtaa ggccggagtt ttatctcgtc acagagtaaa ttttgctcat
    31381 gattgacagc ggagtttacg ctgtaacaga aatattatgg tgaggaactg tttttttatc
    31441 cagtataatt tgttggcata attaagtacg acgagtaaaa ttacatacct gcccgcccaa
    31501 ctccttcagg tagcgactca tgagtaaacc gttcaaactg aattccgctt ttaaaccttc
    31561 tggcgatcag ccagaggcga ttcgacgtct cgaagagggg ctggaagatg gcctggcgca
    31621 ccagacgtta cttggcgtga ctggctccgg gaaaaccttc accattgcca atgtcattgc
    31681 tgatcttcag cgccctacca tggtacttgc gcccaacaaa acgctggcgg cccagttgta
    31741 tggcgaaatg aaagagttct tcccggaaaa cgcggtggaa tatttcgtct cctactacga
    31801 ctactatcag ccggaagcct atgtaccgag ttccgacacc ttcattgaga aagatgcctc
    31861 ggttaacgaa catattgaac agatgcgttt gtccgccacc aaagcgatgc tggagcggcg
    31921 tgatgtggtt gtggtggcgt ctgtttccgc gatttatggt ctgggcgatc ctgatttata
    31981 tctcaagatg atgctccatc tcacggtcgg catgattatc gatcagcgcg cgatcctgcg
    32041 ccgactggcg gagctgcaat acgctcgtaa tgatcaggca ttccagcgtg gtactttccg
    32101 cgttcgtggt gaggtgattg atatcttccc ggcagaatcg gatgacattg cacttcgcgt
    32161 ggagctgttt gacgaggaag tggaacgatt gtcgttattt gacccgctca ccgggcagat
    32221 tgtttccact attccacgtt ttaccatcta cccgaaaacg cactacgtca cgccgcgcga
    32281 gcgcatcgta caggcgatgg aagagatcaa agaagaactg gccgccagac gcaaagtgct
    32341 gttggagaac aacaagttgc ttgaagagca gcggctgacc cagcgtaccc agtttgatct
    32401 ggagatgatg aacgagctgg gctactgttc cgggattgag aactactcgc gcttcctctc
    32461 cggtcgtgga ccgggtgagc caccgccgac gctgtttgat tacctgcctg ccgatgggct
    32521 gctggtggtc gatgaatcac acgtcaccat tccgcaaatt ggcggcatgt atcgcggtga
    32581 ccgggcgcgt aaagagacgc tggtggagta cggcttccgc ctgccatcag cgctggataa
    32641 ccgtccgctg aaatttgaag agttcgaagc attagcgccg caaaccatct atgtttcggc
    32701 gacgccgggt aattacgagc tggaaaaatc cggcggcgat gtggtggatc aggtggtgcg
    32761 tccaaccgga ttgcttgacc cgataattga agtgcggccg gtggcgacac aggttgatga
    32821 tcttctttcg gagattcgtc agcgagcggc aattaacgaa cgcgtactgg ttacaactct
    32881 gaccaagcga atggcggaag atctcacgga atatctcgaa gaacacggcg agcgcgtgcg
    32941 ttatcttcac tccgatatcg acaccgtcga gcgtatggag attatccgcg acttgcgtct
    33001 gggtgagttc gacgtgctgg tagggatcaa cttactgcgc gaaggtctgg atatgccgga
    33061 agtgtcgctg gtggcgatcc tcgacgctga caaagaaggc ttcctacgtt ccgaacgttc
    33121 gttgatccag actattggtc gtgcggcgcg taacgttaac ggtaaagcga ttctctacgg
    33181 cgataagatc accccgtcaa tggcgaaagc gattggcgaa accgaacgtc gtcgcgagaa
    33241 acagcagaag tacaacgagg aacacggtat tacgccgcag ggcttgaaca agaaagtggt
    33301 cgatatcctg gcgctggggc agaacattgc taaaaccaaa gcgaagggca gaggaaaatc
    33361 gcgaccgatt gttgagccgg ataatgtgcc gatggatatg tcgccgaaag cgttacagca
    33421 gaaaatccat gaactggaag ggctgatgat gcaacacgcg caaaatctgg agttcgaaga
    33481 agcggcgcaa attcgtgacc agttgcatca gctgcgtgag ttgtttattg ccgcgtcgtg
    33541 acaagatgac gaagccggaa tattcttccg gcttcgtgtt aacctaaagc ctgtaatgcc
    33601 ttttccagcg cgttatgtaa caactggcgg tcatgacgat acggaatatc gctggcctcc
    33661 agtacctcct gaatcacaat ccgctctttc accgccgaga catccacttt tggcccgacg
    33721 atgaccgcat caatgacctt tttaccgaca tactgctcca taattgccag cttgctttcc
    33781 agcttcaaat tagccgcagg taaactcaac tcacgcccca gattgccgat atagaccatc
    33841 ggcgctggcg tgcggcgtaa tgcttgtgcg atttcattca gcagcagaat tggcatcagg
    33901 ctggtataaa aactgccagg cccaataatg atgagatccg cttcactgat agcgtgaaca
    33961 gcctcacgcg ttgcgggtac attcggcgtt aacagtaact cctgaatcgg cgtagttaac
    34021 tggtcgatat tgacctcgcc gtatacttca tgcccctgat cgtcaatcgc cattagatcc
    34081 accggatgct ccgacatcgg aatcaaatgt gcgtccactt tcagcagatt gcgaatcaga
    34141 ttgatcgctt caagaggtcg cacgctaagg tgatccagtg ccttcagcat caggtttcca
    34201 aggttgtgcc cggaaagttc gccattaccg ccgaaacgat attcaaacat tgcggaagcg
    34261 acgctgggtt cagttatcag ctggttgagg cagttacgca tatcgcccca ggcaataccg
    34321 ccttctgagc ggcgaatacg ccccgtcgag ccaccattat ccgtggtggt gacgataccc
    34381 gttaaacgag aacccaaaga cgaaagtgat gagagaacgc gtcccagtcc atgccctccg
    34441 ccgagagcaa cgacacgatc aaggtcagcc agcgtacgat tgcgcatata gtttcctgga
    34501 gtcagattat ccgcgctaca gtagcgcaaa ttgcggggaa acagcaatta acctgccaat
    34561 attgaggata caaactattt tcttctacct ctaaaggacg atgcacgcta tgcctctctg
    34621 atgatgtata tcaaagtaaa accgccattt tcccttattc tgtagcgaaa tagcacgatc
    34681 atgacgctat atacatgatt acatagcgaa agtgtggatg gtaaaaatct catttacgcg
    34741 ctagtatcgg cttaaccact aaacactcta gcctctgcac ctgggtcaac tgatacggtg
    34801 ctttggccgt gacaatgctc gtaaagattg ccaccagggc gaaggaagaa atgacttcgc
    34861 ctcccgtatc tggaaaggtg tacatggctt cacaactgac tgatgcattt gcgcgtaagt
    34921 tttactactt gcgcctgtcg attactgatg tgtgtaactt tcgttgcacc tactgcctgc
    34981 cggatggcta caaaccgagc ggcgtcacca ataaaggctt tcttaccgtc gatgaaattc
    35041 gccgggttac gcgcgccttt gccagtctgg gcaccgaaaa agtgcgcctg acaggcggtg
    35101 agccgtcttt acgccgcgac tttaccgata tcatcgccgc tgtgcgggaa aacgacgcta
    35161 tccgccagat tgcggtcacc accaatggtt accgtctgga acgcgatgtg gcgaactggc
    35221 gcgatgcggg acttactggc atcaacgtca gcgttgatag ccttgacgct cgccagtttc
    35281 atgccattac cgggcaggat aaattcaacc aggtcatggc agggatcgat gctgcatttg
    35341 aggccggttt tgagaaggtc aaagtcaata ccgtgctgat gcgtgatgtt aatcatcatc
    35401 agctcgacac ctttctgaac tggatccagc atcgccctat ccagctgcgt ttcatcgaat
    35461 tgatggaaac gggcgagggc agcgagctct tccgtaagca tcacatctct ggtcaggttc
    35521 tgcgtgacga gctactgcgt cgcggctgga tccaccaatt acgtcaacgc agcgacggtc
    35581 ccgcgcaagt cttttgtcat ccggattacg ccggagagat tggccttatc atgccgtatg
    35641 aaaaagactt ctgcgccact tgcaaccgcc tgcgcgtttc ctccattggt aaactccatc
    35701 tctgcctgtt tggtgaaggc ggcgttaacc tgcgcgatct gctggaagac gatacccagc
    35761 aacaggcgct ggaagcgcgt atttcagcgg cgctgcggga gaagaaacag acccatttcc
    35821 tgcaccaaaa caacaccggt attacgcaaa acttatcgta cattggcggc taaaacgtca
    35881 aaaggagaga tcagatgagt caggtaagca ctgaatttat cccgacccgt attgctattc
    35941 ttacggtttc taatcgtcgt ggtgaagaag acgatacctc cggtcactat ctgcgcgatt
    36001 cggcgcagga agcgggccat cacgttgtcg ataaagccat tgtgaaagaa aaccgctacg
    36061 ctattcgcgc tcaggtatct gcgtggatcg ccagcgacga tgtacaagtg gtattgatta
    36121 cgggggggac tggcctgacg gaaggagatc aggctcccga agcgctgctg ccgttgttcg
    36181 accgtgaagt tgaaggtttt ggtgaagtgt tccggatgtt gtcgttcgaa gagattggca
    36241 cttccacgtt gcaatctcgt gcggtagcgg gcgtcgctaa caaaacgctg attttcgcca
    36301 tgccgggttc gaccaaagcg tgccgtaccg catgggaaaa tatcattgcg ccgcagctcg
    36361 atgcccgtac gcgtccgtgt aatttccatc cacatttgaa gaaataagta tgtcgcaact
    36421 gacccatatc aacgccgctg gcgaagcgca catggtggat gtctccgcca aagcggaaac
    36481 cgtgcgtgaa gcgcgcgccg aagcctttgt caccatgcgc agcgagacgc tggcgatgat
    36541 tattgatggt cgccaccaca aaggcgacgt atttgccact gcgcgtatcg ccggtattca
    36601 ggcggcaaaa cgcacctggg atctgatccc gctgtgtcac ccgctgatgc tcagcaaagt
    36661 tgaagtcaat ttacaggccg agccggagca caatcgggta cgtatagaaa ccttatgccg
    36721 cctgaccggg aaaaccggtg tcgaaatgga agcgttaacc gcggcctccg tggcggcgct
    36781 gaccatttat gacatgtgca aagcggtgca aaaagatatg gtaattggtc cggtacgttt
    36841 gctggcgaag agtggcggca agtcgggtga ctttaaggtg gaagcggatg attaaagttc
    36901 tttttttcgc ccaggtgcgc gagttggtgg gaacagatgc aaccgaagtg gctgcagatt
    36961 tcccaaccgt tgaagcgtta cgccaacatc tggctgcgca gagcgatcgc tgggcgctgg
    37021 cgctggaaga tggcaaattg ctggctgccg tcaaccagac gctggtgagt tttgaccatc
    37081 cgctgactga cggcgacgaa gtggctttct tcccgccggt aaccggaggt taagatggca
    37141 gaaaccaaaa ttgttgtcgg cccgcagcca ttcagcgtag gagaagagta tccgtggctg
    37201 gcggagcgtg atgaagacgg tgcggtagtc acctttaccg gtaaggtgcg caaccataac
    37261 cttggcgaca gcgtcaaagc attaaccctc gaacactatc cggggatgac tgaaaaagca
    37321 ctggcagaaa ttgtcgatga agcgcgtaac cgctggccgc tggggcgcgt cacggtgatt
    37381 caccgcatcg gggaattatg gccgggcgat gaaatcgttt ttgtcggtgt caccagtgcg
    37441 catcgcagca gtgcgtttga agccgggcag tttattatgg attatctcaa aacccgcgca
    37501 ccgttctgga agcgcgaagc cacgccggaa ggcgaccgct gggtcgaagc ccgggagagc
    37561 gatcagcagg cggcaaaacg ctggtagttt tttgttagcc ggataaggca cagctgcgtc
    37621 cggcaatttc accgctcagt acatgtgttt acgctgcgtc gatgcacagc ctcatcattt
    37681 tgcagtatcc ttaagatatt ccttatatct tcaggagatc gtcatggaca gattcccacg
    37741 ttctgattca attgtacaac tccgggctgg cttgcaaact tatatggcgc aagtctatgg
    37801 ctggatgacc gttggcttgt tgctgaccgc atttgttgcc tggtatgcgg ctaattccgc
    37861 ggccgtgatg gagctgttgt tcactaaccg tgtcttttta atcggtctga tcatcgcgca
    37921 attagcattg gttattgtgt tatcagcgat gattcaaaag ctgagcgcag gtgtaacgac
    37981 gatgctcttt atgctttatt cggcgctgac gggtcttacg ctttccagta ttttcattgt
    38041 ctataccgct gcttctatcg ccagtacttt cgtcgttact gccgggatgt ttggcgcaat
    38101 gagcctgtac ggttacacca cgaagcgcga tctaagcggc tttggcaata tgctgtttat
    38161 ggcgttaatc ggcattgtgc tggcatcgct ggtcaacttc tggctgaaaa gcgaagcatt
    38221 gatgtgggca gtcacctaca tcagcgtgat tgtctttgtc ggattgacgg cgtatgacac
    38281 tcagaaattg aaaaatatgg gtgagcagat tgatacccgc gacacgtcga acctgcgcaa
    38341 atattccatt cttggcgcgt taaccttgta tctggacttc atcaacctgt tcctgatgtt
    38401 gctgcggatc ttcggcaacc gccgttaatt ctttttcacc atcaatatcc cggccatgtg
    38461 tcgggatttt
//
LOCUS       DAPOZO010000046        37470 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPOZO010000046 DAPOZO010000000
VERSION     DAPOZO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.046"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(188..589)
                     /gene="nikR"
                     /locus_tag="ROI21_002456"
     CDS             complement(188..589)
                     /gene="nikR"
                     /locus_tag="ROI21_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDX5111368.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(594..1400)
                     /gene="nikE"
                     /locus_tag="ROI21_002457"
     CDS             complement(594..1400)
                     /gene="nikE"
                     /locus_tag="ROI21_002457"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDX5111369.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAYPVRRRATEKV"
     gene            complement(1397..2161)
                     /gene="nikD"
                     /locus_tag="ROI21_002458"
     CDS             complement(1397..2161)
                     /gene="nikD"
                     /locus_tag="ROI21_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDX5111370.1"
                     /translation="MPQQIELRDIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2161..2994)
                     /gene="nikC"
                     /locus_tag="ROI21_002459"
     CDS             complement(2161..2994)
                     /gene="nikC"
                     /locus_tag="ROI21_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDX5111371.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(2991..3935)
                     /gene="nikB"
                     /locus_tag="ROI21_002460"
     CDS             complement(2991..3935)
                     /gene="nikB"
                     /locus_tag="ROI21_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDX5111372.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(3935..5509)
                     /gene="nikA"
                     /locus_tag="ROI21_002461"
     CDS             complement(3935..5509)
                     /gene="nikA"
                     /locus_tag="ROI21_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDX5111373.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQVGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5620..6207)
                     /gene="acpT"
                     /locus_tag="ROI21_002462"
     CDS             complement(5620..6207)
                     /gene="acpT"
                     /locus_tag="ROI21_002462"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDX5111374.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6262..7311)
                     /locus_tag="ROI21_002463"
     CDS             complement(6262..7311)
                     /locus_tag="ROI21_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDX5111375.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHISPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7443..8660
                     /locus_tag="ROI21_002464"
     CDS             7443..8660
                     /locus_tag="ROI21_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5111376.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8664..9221)
                     /gene="dcrB"
                     /locus_tag="ROI21_002465"
     CDS             complement(8664..9221)
                     /gene="dcrB"
                     /locus_tag="ROI21_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDX5111377.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9294..9959)
                     /locus_tag="ROI21_002466"
     CDS             complement(9294..9959)
                     /locus_tag="ROI21_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDX5111378.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10180..10425
                     /gene="tusA"
                     /locus_tag="ROI21_002467"
     CDS             10180..10425
                     /gene="tusA"
                     /locus_tag="ROI21_002467"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDX5111379.1"
                     /translation="MTDLFSCPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10528..12726)
                     /gene="zntA"
                     /locus_tag="ROI21_002468"
     CDS             complement(10528..12726)
                     /gene="zntA"
                     /locus_tag="ROI21_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDX5111380.1"
                     /translation="MSTPDNHGKKAPHFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQTSRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPAAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPAVAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(12800..13426)
                     /locus_tag="ROI21_002469"
     CDS             complement(12800..13426)
                     /locus_tag="ROI21_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDX5111381.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13567..13926
                     /locus_tag="ROI21_002470"
     CDS             13567..13926
                     /locus_tag="ROI21_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDX5111382.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(13929..14198)
                     /locus_tag="ROI21_002471"
     CDS             complement(13929..14198)
                     /locus_tag="ROI21_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDX5111383.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRTFLFGVFELLVWQKKFKVKK"
     gene            complement(14188..14784)
                     /gene="rsmD"
                     /locus_tag="ROI21_002472"
     CDS             complement(14188..14784)
                     /gene="rsmD"
                     /locus_tag="ROI21_002472"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDX5111384.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPNVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            14934..16430
                     /gene="ftsY"
                     /locus_tag="ROI21_002473"
     CDS             14934..16430
                     /gene="ftsY"
                     /locus_tag="ROI21_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDX5111385.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVPAQEP
                     AKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16433..17101
                     /gene="ftsE"
                     /locus_tag="ROI21_002474"
     CDS             16433..17101
                     /gene="ftsE"
                     /locus_tag="ROI21_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDX5111386.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17094..18152
                     /gene="ftsX"
                     /locus_tag="ROI21_002475"
     CDS             17094..18152
                     /gene="ftsX"
                     /locus_tag="ROI21_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDX5111387.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18397..19251
                     /gene="rpoH"
                     /locus_tag="ROI21_002476"
     CDS             18397..19251
                     /gene="rpoH"
                     /locus_tag="ROI21_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDX5111388.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19523..20626
                     /gene="livJ"
                     /locus_tag="ROI21_002477"
     CDS             19523..20626
                     /gene="livJ"
                     /locus_tag="ROI21_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDX5111389.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(20826..21209)
                     /gene="panM"
                     /locus_tag="ROI21_002478"
     CDS             complement(20826..21209)
                     /gene="panM"
                     /locus_tag="ROI21_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDX5111390.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGM
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            21633..22742
                     /gene="livK"
                     /locus_tag="ROI21_002479"
     CDS             21633..22742
                     /gene="livK"
                     /locus_tag="ROI21_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDX5111391.1"
                     /translation="MKRNAKPIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            22790..23716
                     /gene="livH"
                     /locus_tag="ROI21_002480"
     CDS             22790..23716
                     /gene="livH"
                     /locus_tag="ROI21_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDX5111392.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            23713..24990
                     /gene="livM"
                     /locus_tag="ROI21_002481"
     CDS             23713..24990
                     /gene="livM"
                     /locus_tag="ROI21_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDX5111393.1"
                     /translation="MKPMHIAMALLSAVMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WEWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            24987..25754
                     /gene="livG"
                     /locus_tag="ROI21_002482"
     CDS             24987..25754
                     /gene="livG"
                     /locus_tag="ROI21_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDX5111394.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            25756..26469
                     /gene="livF"
                     /locus_tag="ROI21_002483"
     CDS             25756..26469
                     /gene="livF"
                     /locus_tag="ROI21_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDX5111395.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINEGEIVTLIGANGAGK
                     TTLLGTLCGDPRATGGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            26867..28183
                     /gene="ugpB"
                     /locus_tag="ROI21_002484"
     CDS             26867..28183
                     /gene="ugpB"
                     /locus_tag="ROI21_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDX5111396.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHITMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            28281..29168
                     /gene="ugpA"
                     /locus_tag="ROI21_002485"
     CDS             28281..29168
                     /gene="ugpA"
                     /locus_tag="ROI21_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDX5111397.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            29165..30010
                     /gene="ugpE"
                     /locus_tag="ROI21_002486"
     CDS             29165..30010
                     /gene="ugpE"
                     /locus_tag="ROI21_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDX5111398.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAT
                     PMTLIPGPHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            30012..31082
                     /gene="ugpC"
                     /locus_tag="ROI21_002487"
     CDS             30012..31082
                     /gene="ugpC"
                     /locus_tag="ROI21_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDX5111399.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            31079..31822
                     /gene="ugpQ"
                     /locus_tag="ROI21_002488"
     CDS             31079..31822
                     /gene="ugpQ"
                     /locus_tag="ROI21_002488"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDX5111400.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTSPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(31809..32249)
                     /locus_tag="ROI21_002489"
     CDS             complement(31809..32249)
                     /locus_tag="ROI21_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDX5111401.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            32369..34111
                     /gene="ggt"
                     /locus_tag="ROI21_002490"
     CDS             32369..34111
                     /gene="ggt"
                     /locus_tag="ROI21_002490"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDX5111402.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPPPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRNMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALEK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLKK
                     GDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGEYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVAKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(34149..34433)
                     /locus_tag="ROI21_002491"
     CDS             complement(34149..34433)
                     /locus_tag="ROI21_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HDX5111403.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFESQEFL
                     ESGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(34672..34788)
                     /locus_tag="ROI21_002492"
     CDS             complement(34672..34788)
                     /locus_tag="ROI21_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111404.1"
                     /translation="MEKLTTELHSLSEMDRRHVISILAEIANGYDDFSDMLI"
     gene            complement(34883..35377)
                     /locus_tag="ROI21_002493"
     CDS             complement(34883..35377)
                     /locus_tag="ROI21_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111405.1"
                     /translation="MMTKTQINKLKKMMNDLDYPFEAPLKESFIESIIQIEFNSNSTN
                     CLEKLCNEVSILFKNQPDYLTFLRAMDGFEANGLRLFSLSIPEPSVKNIFAVNEFYRN
                     NDDFINPDLQERLVIGDDSISIFTYDIKNNFFEIRDNIGTENIFSSFSDFSSFLNEIM
                     DSCS"
     gene            complement(35374..36552)
                     /locus_tag="ROI21_002494"
     CDS             complement(35374..36552)
                     /locus_tag="ROI21_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HDX5111406.1"
                     /translation="MSNIVYLTVTGEQQGNISAGCGTTESIGNRWQSGHEDEIFTFSL
                     ANRITNTGLGSQLQGMTFCKLIDKSTPLLINSINNNEQLFMEFDFYRINRFGRWEKYY
                     YIQLRGAFLSAIHHQIIQNQLDTETITVSYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGVLVGESKITPWAIPSGSIYPPMKNIMEPTK
                     "
     gene            complement(36787..37275)
                     /gene="yhhY"
                     /locus_tag="ROI21_002495"
     CDS             complement(36787..37275)
                     /gene="yhhY"
                     /locus_tag="ROI21_002495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDX5111407.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFIDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         8867 a         9490 c        10197 g         8916 t
ORIGIN      
        1 tattatttta atgacagtat cttcatacga aaaaacgaag aaaaacttgt ggtcacaatg
       61 aattgaaggt ttttaggaat atatttagaa cgttacaaaa gagaaggttc ggatagggta
      121 tttagagagg aatcaggtgt gctagtggcc ctttgttgat cgttcaccaa aggacccaat
      181 aataggctta atcttccttc ggcaagcact gcaaatgccc gtgccgcacg ccgcgctggg
      241 cgataacgtc atcggcaaaa tgctgcacgt cacccatgtc acctttcaac acggcgattt
      301 ccagacagtc gtcgtgattg atgtggacat gtagcgtcgc gacggagaga tcgtggtgat
      361 ggtgctgggt ggagacaata cggctggcta agtcgcgttt ttcgtgttca tacacatacg
      421 acagcaccgc gaaaccttgt gtgccgtgct gctgagtggc ctcttgcgcc agggcgctac
      481 gcagaatatc gcggatagct tcggaacggt tgttatagcc acgacgctgg ctcaggctgt
      541 ccagcgtctc cagtaaatcg tcatcaagcg tgatggtaac tcgttgcata gacttaaacc
      601 ttttctgtgg cgcgacggcg cacggggtat gcgggtaata ccgcgttttg tagcacacgt
      661 ccggcgtcag aggaaaaggt taatttatct cccaccacct gggtttcgac gatttgcccg
      721 ttgtccatca ccatcactcg ctggcaaaag cgttccacta ggcgtaagtc atgagtgatg
      781 aacaggcagg cagtgccaaa ctgttgttgt agctttttca gcaggcgaat gacgcccgcc
      841 tgtaacacga gatcaaggtt agaaacggct tcatccagaa tcagcagttt cggttcgacc
      901 gccaacgcgc gtgccaggca gacgcgctgg agctggccgc cgcttaactg cggtgggcgc
      961 ttgtcgagca cgctgtcatc gagatcgacc gccttcagca tttcgctggc gcgcgccagt
     1021 tgttcggctt ttttcagcga tagcaggtgg cgcatcggtt cacgcaggat ctcgcgcacg
     1081 gttttgcgcg gattcacggc actgatggag tcctgaaaca ccatctgaat atcacggcgg
     1141 aaggctttac gttgggcgcg attaagtttc gccagcgatt cgccgcgcca gctaatattc
     1201 ccttgtgagg gagactctaa gcctaccagc agccgcgcga gggtgctttt tccgcagccg
     1261 ctgcgtccca gcagggcgac ggtttcaccg cttttcaggg tcagggaaac gttattcagt
     1321 accgcctgat gctgatgttt tccgctaaat ccaccgtgcg catagtgatg tgaaaggtcg
     1381 cagacgttaa gtaaagtcat gatgccagct ccataccgta gagggcgaga tgagcggaaa
     1441 ccaggctgcg cgtcaccgta tgtttggggg cgttaaacag cgtttctaca tcgccctgtt
     1501 cgacaatttt accgtgtgac atcaccgcca cgtcatccgc cagacgcgcc acaacgccca
     1561 tatcatgggt gaccagcagc attcccggtg cttgtttttg cataatgctt tccagcagat
     1621 cgaggatgcg cgcctgtgct accacgtcga ggtcggtggt cggttcatcg gcgatgataa
     1681 acggcgattc acacaacacc gccatcgcaa tcatcatgcg ctgcaacatg ccgccgctca
     1741 tctcgaacgg gtacagcttc aacacgcgcg cggcgttttc caaccccacc gcttctatgg
     1801 cagcggtaag cgtagcgtca tcggcgggtt tccccagcgc caggcaggtt tcacgcgcgt
     1861 gggtgtgcat ggtgtgtagc ggattaaagg cgctgcgcgg gttctgcatg atggtggcaa
     1921 ttttgatgcc gcgcagggca cagggagaaa ccggtttgcc atcggctaaa atttcccccg
     1981 ccgtctggcg aacgcctgcg ggcagaatgc ccagcgtcgc ggcgcaggtc agtgatttcc
     2041 cgctgccgct accgccgact aacgccagca cacgcccgcg tttaagggtt aacgatacgc
     2101 cgtgcaccag cggctgcgcg gcctgtagcg cgatatcgcg cagttcaatc tgttgcggca
     2161 tcagtgtgcg tgctccgtca ccagatgagg atccagatga tcgcgcagtg cgtcacccac
     2221 caggttaaag gccatcacgc tgataaacag cgccagcccc ggccagaaca tttgcagcgg
     2281 ctgggtccag atatactgac gcgcgtcgtt aatcatcacg ccccattcgg cggtcggcgc
     2341 ggtcacgccg aggccgagga aggacatccc cgcgacgtgg agcatcatat gaccaatatc
     2401 cagcgtcgcc agcaccagca gcgaggggat caccgcgcct gccagatgat cgacaaacac
     2461 ccgcacatgg cccgcgccgg aaagccgtga cgccagcaca aactcgcgct ggcgtagcga
     2521 aatcaccagg ctgcgcacca tgcgcgcata ccacgcccag tgcgacaggg cgatggcgat
     2581 aatcacgttg gtcaacccgg tgccgagtac gccgaccata aagaacgaca gaatcgacgt
     2641 cgggaaggtc ataaacatat ccgcgacgcg catggtggcc tgatcaacgc gcccgccaat
     2701 cagcccggcg ctgccgccaa taactagccc taatgtcagc accagcagca ggcaggccat
     2761 taccgagcct agcgacacgc gggtcgccgc catcagccgc gagaaaatat cgcgccctaa
     2821 atgatcggta cccagccagt gctgcgcatc cggtgaaagc aggcgcgacg gcagatcaat
     2881 cgcctgtggg tcatacggca gccaccactg gctggtgagc gcaatcagcg ccagcagggc
     2941 gataatgatc agcgccaggc gtaccgacca gcgggaagag aggaaaaagt tcacgcgtgg
     3001 gctccttcat gacgacgaat gcgcgggtcc agcgcggcgt tgagcaaatc aacaatcaaa
     3061 ttacagacca caaaaaccac caccatcatc agcgtaaagc actggatcac cggatagtca
     3121 cggttaaaaa tcgccgacac cgcatagcgc ccgacgcccg gccaggcaaa gatgttttcg
     3181 ataatcatcg tcccgccaat cagttcgcca atgtgcatcc ccacggcggt gatcatcggc
     3241 agcgaggcat tgcgcagaat atgacgacgc tcggtctgtt tgtcattcag gccgcgtagc
     3301 cgcgcccagg tgacgtgacg ctgaccggcg acgtccagca tactggcgcg cagcagtcgc
     3361 gcgttaatcg ccagcgacat aaaggcaatg gaaaccgcag gcaaaatgat gtgctgccag
     3421 ccgccgtagc ccatcgcggg tagccattgc agatacaccg aaaacgccat caccagtaaa
     3481 aacgccagcc agaagttagg catcgacacg ccgagaaacg cgatgaaacg tacggcgaaa
     3541 tccggcagac ggtcgcgatg gcgcgccgcc cagataccga gcggcacgga agtgagcaga
     3601 attaatacca gcgcggcacc tgcaagttcc agcgtggcgg gcaggaagtt cagcatatcg
     3661 tccagcaccg ggcgttggct ggcgaatgag ataccaaagt caagatgcag cgccttccac
     3721 aaccaggtgc cgtactggac gtacagcggc tgatccagcc ccagcatggt gcgtgtagag
     3781 gccagcatct ccggcgtcgg cggcaggtta gacagacgca aataatcaag cgccgggtcg
     3841 ccggtgccga ggcgcagcat cagaaaaatg atcaccgagg cggcgagcac catcgggatc
     3901 agcagcagaa agcggcgtaa tacgtaacgc aacattaagg tttcaccggt ttaatctgtt
     3961 cgaacggaat ttcggtggcg atcggcgcgt aggggatgtt acccagctcc ggttttgata
     4021 ccaccatcat cgagatgtaa ctgataggca gataaaccgc ctcgtcatgc agacgggtca
     4081 gaatgtcgcg atacagcgcc tgacgttgcg tttcgtcatg ggttgccagc acttcgccga
     4141 tctctttatc aatcagcggc ttgtcggcta atccttgctg tgcctggaaa tcagcgtgcg
     4201 acggtacgcg cattgaactc aggaaggcgt gtggatcgta tggcgcgccc caggtgcggt
     4261 ggaaaatcat gccaaaacga ccgtcgcgct gacgagcata gatactgctc tcttcttcgc
     4321 caatcagcga gacatctgcg ccaacctggc gcatatcagc ctgaatgatt tccgccatcg
     4381 atttacttaa cgcatcggtg ccgatgaacg acagttcaat gcgcagcggc tgaccatttt
     4441 tctcgcggat gtctttgccc gcaggcaacg tccaaccggc tttttccagc aacgctttcg
     4501 ctttctgcgg atcgtactgg cgcggtttca ggccgaggtt ggcgtagggt acagaaggag
     4561 caaacagggt gtcggcgacc tgctgggtgc catacaacgc gttatcaatc agcgattttt
     4621 tgtttaccgc gtaattaaga gcttcacgta cggccagctc gttggtgggg gctttggcgg
     4681 tattgagcgc cagcatcacg gtttcgatcg gctgtgacag ttgggtgtgg taagccgggt
     4741 tctggctaaa gcgggcgaag gtatcgagcg gtaataaccc ttcgtttccg tacagcaggt
     4801 cgatatcgcc agtttcaaac gccaccgcgc gggtagtcgg gtccgggatc acgttaaagg
     4861 tgatcttttt aatcgctggc ttttcgcccc agtagttttc gttacggacg aagacatcgt
     4921 actgattcag tttcgattcc tgtaaaaccc acggcccggt gccaatcggc gctttaattc
     4981 cgttcatggt ttcgtggttt ttaaactgcg agggtgcgat aaaacggaaa ggacggggca
     5041 gggccagttc ttgcaggaaa ggatagtagg cgcttttcag ggtgatttgc agctcgtttt
     5101 tactgagtgc tttgacatca acaatctggt ttgccagctc cagccaggcg tgacgttgac
     5161 ggttatcgag cactgcgcgg aagttttctg ccgccgcctc tgcatcaaac ggttcgccgt
     5221 tggagaattt cacgtcatca cgcagggtga aggtccaggt tttgccatct tcagaatgag
     5281 tccagctttt tgccagccac gggatcaccg aaccgtctgc ctgatatttc accaatggtt
     5341 cataaaccat gctctgggcg aacatctggt taggcgtgta aaggtgcggg tttagtggcc
     5401 cgacattcac cggccaggcg gtggtgattt catctggtgc agcggcatgg acgataaaag
     5461 acgcacaagc cagcagcgca aatagagtgc ggcggagtgt agagaacatg gtaaccccaa
     5521 tggattaaaa tagatggcgg aaataagtat gacgatttta agtattcgtc atactgatta
     5581 cctgttctgg atcaatagat gggcagtcgg gtgggcggat cagttaactg aatcgatcca
     5641 ttgcacactg tcggcggtga gcgtaaaggg ggtaggggtg cagatcgcca ggcttaaatt
     5701 ttccagctga caatggctga ctgacagcga ggagttatag gtgctgtcta cgctgacgat
     5761 ttgccaggcg ctgccgccac gctgtttaac gatggcttct ttgcgcgtcc agatgcgcca
     5821 gaacatttcc agttgctgat caggatgcac ggcgtccatc tcagcgtgtt ccccgaggct
     5881 gaacacggcg ttcgccagcc agcgccagtt ggcgcgcggg cgaatcactt cgatatcgca
     5941 gccgacttcg ccttcatcgc tcaacagcag ggctatatcg tcaccgctat ggcttaagtt
     6001 gaaccatagc ggcgtttccg gcgcgaatgc cggtttgcct tgttcgccat agatgatctc
     6061 cggtagcggg gaaagcgtgt gcgaaagcaa tgcacgcccc gccagccagc gttcacgtcg
     6121 tggaccttgc ggtgcttgct cgcgtaaacc cggtggcaga ggagctgcgc ttaaggtcga
     6181 aactttcccc agaactatcc gatacatatc agggccaacg tttaatggaa aatgaaagtg
     6241 cgtatcgtaa cacttgtcgc ctcatcccgg taaccgactt ttcggtctgc ctggtcccag
     6301 taaaatcgcc agtttgctac cgcctttggt ggtttccatc cagattttac acacgctggt
     6361 taatggcacc gaaagtagca tccctaccgg gccgagcagc catccccaaa ttaacaatga
     6421 aagaaatacc accatggtgg acatccccag gcgatggccc atcatccgtg gttctaaaat
     6481 attgccgatg accatatgga caactaaaaa caatgcgccg accagaatac attcgtaaat
     6541 accattaaac agcagcacct gaatcattgg cggtacggcg gaaattaccg cgccgatatt
     6601 gggcacgtag ttgagcaaaa acgccagtac tgcccacatc agcgcaaact gcacccccat
     6661 cagctccagc cccagccaga cgatgacacc tgtccataaa ctgagtaacg tctttaacgc
     6721 cagataatgc gaaacgcctt taagtgcgcg gtgtaatccc gcgatgtgaa tctgtgggtt
     6781 atttagcgca aaacgcattt tgtaagggac gtggcgcact tcaaacagca tgaaaactac
     6841 ggtcatcacc agcaaaagca cgctcgccat tgccccggaa agcccggtca ttagcgccgt
     6901 ggtgaaggta accacttttt ccgagtccat ccgctgcagt attcgctccg gcgatatatg
     6961 caaattaaga aaaggcaaca tctcctgcaa tttaaaaagt ttgcgcgtca gctccttatt
     7021 aaacttcggc agcatagaga taaattcgtt aaacgatgcc gccagtacgc cgaccagcgc
     7081 ggtgagtgcg atcagcatca ccaccaccac aatcgtaatg gcaacggggc gttgtactcc
     7141 ccgacgaata aaccaggtaa cgagcgggtt gaggacgatg gcaaaaaaca gcgccagtaa
     7201 cagctgcaca atgatatctg ccgctgcgtg aatgcccgcg aggatcacta ccagcgaggc
     7261 cagcttgagc agaatgtgca tgcccgtttt atcgggttga ggggtttcca ttggggcttc
     7321 cttgtgactt tttgtattaa gtgtagcggg agtcacgcca gcaatattct gattcttact
     7381 gctgattttc acgtcagcac gtggtaaaaa tgaaacactg ttgtaaaaat gtggtgatcc
     7441 tcatgcccga acccgtagcc gaacccgcgc taaacggatt gcgcctgaat ttgcgcattg
     7501 tctccattgt catgtttaac ttcgccagct acctcaccat cgggttgccg ctcgctgtat
     7561 taccgggcta tgtccatgat gtgatgggct ttagcgcttt ctgggcagga ttggttatca
     7621 gcctgcaata tttcgccacc ttgctgagcc gccctcatgc cggacgttac gccgatttgc
     7681 tgggacccaa aaagattgtc gtcttcggtt tatgcggctg ctttttgagc ggtctggggt
     7741 atctgacggc aggattaacc gccagtctgc ccgtcatcag cctgttatta ctgtgcctgg
     7801 ggcgcgtgat ccttgggatt gggcaaagtt ttgccggaac gggatcgacc ctatggggcg
     7861 ttggcgtggt tggttcgctg catatcgggc gggtgatttc gtggaacggc attgtcactt
     7921 acggggcgat ggcgatgggt gcgccgttag gtgtcgtgtt ttatcactgg ggcggcttgc
     7981 aggcgttagc gttaatcatc atgggcgtgg cgctggtggc cattttgttg gcgatcccgc
     8041 gtccgacggt aaaagccagt aaaggcaaac cgctgccgtt tcgcgcggtg cttgggcgcg
     8101 tctggctgta cggcatggcg ctggcactgg cttccgccgg atttggcgtt attgccacct
     8161 ttatcacgct gttttatgac gctaaaggtt gggacggtgc ggctttcgcg ctgacgctgt
     8221 ttagctgtgc gtttgtcggt acgcgtttgt tattccctaa tggcattaac cgtatcggcg
     8281 gcttaaacgt ggcgatgatt tgctttagcg ttgagataat cggcctgcta ttggttggcg
     8341 tggcgactat gccgtggatg gcgaaaatcg gcgtcttact ggcgggggca gggttttcgc
     8401 tagtgttccc ggcattgggc gtagtggcgg taaaagcggt tccacagcaa aatcaggggg
     8461 cggcgctggc aacctacacc gtatttatgg atttatcgct tggcgtgacc ggaccactgg
     8521 ctgggctggt gatgagctgg gcgggcgtac cggtgattta tctggcggcg gcgggactgg
     8581 tcgcaatcgc gttattactg acgtggcgat taaaaaaacg gcctccggaa cacgtccctg
     8641 aggccgcctc atcatcttaa aacttactga ataaccagcg tattaatgat gttttctgcg
     8701 gtggtctgcg ctttttgctg atcgtcagcg ggcagcgtaa tttgcatggt cagcagttga
     8761 ttacccacgt tacccagaat aacggaagag tacgccgtct ggcctttcgc ggagataatg
     8821 ctgtctaact gctgcatttt gtgacctttc agctcaatgg ctttattggt taccacttgc
     8881 agctgcggat cgcgactacg ttgctgatct tccagacgct tcgccagcac cgccagatct
     8941 tctttcgggt catcgcccat gatgacgatg actgctttct gcccggtggc gtcggaccag
     9001 acatgcatgt tattggcctg cgttcccagc ttaccgctct ggtcggtcat atccgctggc
     9061 agcgagaaac ttaacttgcc atcaagcagg ttgacgggat tcccggtagc gttactttcc
     9121 gcgaccgaac cctgcgccgt agcgttagtg tctttatcat cacaggccgc aagccccata
     9181 accagcaggc caattccgac atatttaacc agattgcgca ttgacttctt cctttcgata
     9241 aacggccata acggctcatt catccatctt atcacaactc tgataacgaa cctttaactc
     9301 gcctgcaaag cgttgatttc agatttatct gccagtcttt tcaacagcat attgagtaat
     9361 acgccataca ttggcaggaa gaaaacgata ctgattaaca ctttgaaaca gtaatcgacc
     9421 agcgcgattt ccatccagtg ttcagccata aaggcatcgg ggctacgcca gaaggcaatg
     9481 aagaagaagg ccagtgtgtc gctgacgtta ccgaacagtg tggacgctgt cggtgccagc
     9541 caccagcggc gactctgacg caggcggtta aaaacgtgca cgtcgaggat ctggcccagc
     9601 gcgtaggcca tgaaactggc ggtggcgata cgggcgacaa acaggttgaa gtgagcgagt
     9661 gcgccgaatc cctgccagga gcccatatag aacagcgacg agatgacata ggagatcagc
     9721 aacgccggga tcatcaccgc aaagatgata cgacgggcca gcggtgcgcc aaaaatacgc
     9781 acggtcaggt cggtagcaag gaaaataaac ggaaagctaa atgcgcccca ggtggtatgg
     9841 aaaccaaaaa tggagaccgg aagctgaacc agatagttac tggaggtgat caccagcaga
     9901 tgaaataacg acaaccagaa caacgcctta tagcgttgag tttgcgagaa aacgttcata
     9961 ttgtaccttt ttgattaacc attggggtga gggaacccaa tatgtacgac acgtctgtta
    10021 ccttaacata ataacgtgct tacccttttt tcgagccgcc gcatgatact gctttacgtt
    10081 gacaatgcaa tggttgattt tcacgcaatc gttaacctgg tttgcttacg gactcacagg
    10141 gcgtaaacta gcgccgtttt tttatgtgat gagaagaaaa tgaccgatct cttttcctgc
    10201 cctgaccaca cactcgacgc acttggcctg cgctgcccgg aaccagtaat gatggtgcgc
    10261 aaaacagtgc gcaatatgca gccaggtgaa acgttgctga ttattgctga cgatccggcg
    10321 acgacccgcg atattcctgg gttttgtacc tttatggaac acgaactggt tgctaaagag
    10381 acggatggac tgccttatcg ttatttgatt cgtaaaggcg gttgatgggg gaagattggc
    10441 ttcgatgccg ccttttcccc ctcaccctaa ccctctcccc agaggagcga ggggaccgat
    10501 cgcgctcaat gttgcgatcg gtttgcctta ttttctgcgc aacagcctta acgcattcgc
    10561 cgtcaccagc accgtcgcac cggtatctgc cagcactgcc agccacagtc cggtcatccc
    10621 taacagcgtc gtgacgagga aaacgccctt cagccccagc gcaatagtga tgttctggcg
    10681 gatattggcg tgagtggcgc gtgccagttc aatcatttgc accaggccgc gcaggtggtt
    10741 atgggttaat gctgcatcgg cggtttccag cgccacgtct gtgccgctac ccattgcaat
    10801 cccgatggcg gcagctttca tcgctggcgc gtcgttaata ccatcaccga ccatcgccag
    10861 cggcgcatgt tgattcagct cggtcaccgc tttgacttta tcttccggca agaggcccgc
    10921 tttaaattcc agccccagtt ccccggcgat tgccgccgct gcgcgtggat tatcgccggt
    10981 gaggatcact cctttaactc ccagcgcgtt cagttcactg atggcagttg cagcatcggc
    11041 gcgtagggta tcctgcaatg caattacgcc aagcacgtca tcgttacgca ctaccagcac
    11101 taccgtttgc ccggcgcttt ccagttcatt aatcaaacca gcaaatgcaa cagccggatg
    11161 ttttccggca gcgcatatca acacgcgctc accgttaacc tgcgcttcaa tgccagaccc
    11221 aaccagcgcc cgctgtgatt gggcggcggg aatggcgagt tcagcaacct gtgcttcgcg
    11281 cacaatggct tgcgccagtg gatgcgtcgc gccttgctcg accgccgctg ccagtgtcag
    11341 cagttcggat tcactaatac ccgttgccgg atgaatcgcg gtaacgcgcg gtttaccgac
    11401 ggtcagcgta ccggttttat caaacgccac ctgggtaaca cgacccagtt gttccagcgc
    11461 cgctccgcct ttaattaacg ccccacgacg cgctgctgct gccagcccgg aggtaatcgc
    11521 cgcaggcgtt gagataacta acgcacacgg gcagccaatc agcagcagcg tcagcccttt
    11581 ataaatccac tcctgccagc tggcggcaaa cagcagcggt ggcaccagcg tcaccagcag
    11641 agcgacggcc ataatcgcgg gcgtatagat acggctgaaa cggtcgataa accgctcaat
    11701 gggtgcgcga cgctcttcgg cttcttcgat cagtttcaga atccggtcaa tggcgctggc
    11761 acccggttct gacagcactt ccagcgttac cagacggtct acgctggtgg caccagcagg
    11821 gactttatcg cccgttgcgc gctccaccgg aatggattcg ccggtcaggg cgctttcatc
    11881 aaaactggca aacggtgaga gcagtttacc gtcggcaggc aaacgtccac ctgcggcgac
    11941 ttcaatcaca tcgccagggc gcaggctgtt aatcgccacc tcttcccgct caccgttacg
    12001 caggcgcgtg gcggtttctg gtttcagcgc cattaacgcg ctaacgccct gacgcgcgcg
    12061 gctggcggcc cagccttcca gtcgttcacc aatcaaaaac agcagcaaca ccatcgcagc
    12121 ttcagccgtt gcgccaataa acagcgcacc aatagcggct acgctcatta aggtttcaat
    12181 agcgaagtag ctgccggatt tgatcagccg taatgcctga cgagcaatcg ggtacagccc
    12241 aaccagcgtg gtcgcgataa acgccagttg cccgaacgga tgattaaact gctccagacc
    12301 ccagctgatt gccatcatca cgattagcgt aatcagcggc aaattctctt tcaggcgtga
    12361 tgtttgcggt tcgtcggcgg cctgttcatc gcgcagggaa tagcccgctt tttgcaccgc
    12421 agattcaacc tgggcacgga tgtcattgtc ggcatcgacc accagttttt cggtggcgaa
    12481 caacacctgc acctgattca cgcctgcaag ctggcgcacg gcattttcta ccttgcgcgc
    12541 acaggcggcg cagtccatgc cgctgacttt ccagctatag cgggtgccgg agacgttttc
    12601 agagagcgtt ggcgtgctgg aacatgcgcc gtcgcagcaa cagtcgttgg cgttctgtac
    12661 cgtggttagc ggtttgaacg cagcaaagtg aggggctttc ttgccgtgat tgtcaggagt
    12721 cgacatggca tcctccggtt aagttttttc tcattaaccg aaggatacac tctggagtcg
    12781 actccagagt caagttttat cagagataca gcgagcggac gatgaggaaa tgaccggcaa
    12841 agtagcaggc cgcagcaatc gcgttatctg cccggaagcg tcggcgatag tgactaccca
    12901 gccagacaaa gttactgata aatagcaacg aagcgccgac aaacgcagag agcgccggag
    12961 cggtcggacg gaagaaccac agttcacctg ccagccacac catcaccagc gtcatgccga
    13021 taaacgtgca gataggccaa cgatactctt ccagacgcgt ccagataatc gccagtaaca
    13081 gcgcgcccag aaccagcagt accaacggca gaggccagaa gaaagagagc gtcatctgac
    13141 tggcgaaata gatggtgtac agcaggtgcg agaggaaaaa cgcgccgatg gcgtacatca
    13201 gacgttgacg tggcaacagg gttagcgcat cgcccagcag tgaggcgcac agccccgcca
    13261 gcaccagata gctaatggcg tcgaacatcg gcgcttgcca ggccagtaac aacaggagaa
    13321 gaagggttaa cggtttaaac acccagcgtt gccaggttgg cccacgatac gatgcatcca
    13381 cagatagcca tgcggaaaga cagacagcga taaacgacca aagcatctta gttccttgaa
    13441 ttcgttattt tttcgtaggc ggaatgccta cggtcttgct tccagtttag tggcgagggt
    13501 ggacggatga caacaccata actggcaggg tatgcttaat tccgcatttt ccgatgaggg
    13561 ataaagatga gcaaaccacc tcttttcttt attgttatca ttggcttaat catcgtcgcc
    13621 gcatcgtttc gttttatgca acagcgacgg gaaaaagctg ataatgatat ggcaccgctc
    13681 cagcaaaagc tggtggtggt gagcaacaag cgggaaaaac cgattaacga tcgccgttcg
    13741 cgccagcagg aagtgactcc ggcaggcacc agtatgcgct atgaggcaag cttcaaaccg
    13801 caaagcggag gaatggagca gacgtttcgc ctcgacgccc agcagtacca cgccctgaca
    13861 gtgggcgata aaggtacgct gagctataaa ggaacgcgct ttgttagctt tgttggcgaa
    13921 caataaggtt attttttaac tttaaatttc ttctgccaga ccagcagttc aaacacgccg
    13981 aaaaggaaag tccgcacctt ttcggcggtg gtcatctgcg ggccatcttt cggtaaagtg
    14041 gatttcaata gcgccagctg cataccatgc atcagcacgg taaaaatcag cgccacgtta
    14101 acgaaaatat tcagcgggcg cgggaaggga tgcaccaggt tgaggattaa aaatccccaa
    14161 acgcagagca ttaacaaacg tccaatatta atcagcatca ctttctcctt gtgcttcgcg
    14221 ttgatatagc cgataagcca cctgacccgc cactttttcc cgatgtaatg accagttcgc
    14281 cggaacgttg ggcagaccgt tttcgacttc gctttcgaca taaatcaggg cttcgtcagc
    14341 cagccagccg ttatcttcca gtaaatttat cgtctcttct aacaatccac ggcggaacgg
    14401 tggatcgaca aacacgatat tatgcggtgt gcctttttgc gccaggaatg acatcgcgtt
    14461 gctgttcacc acgcgtgcat tgcctgcttt tagtgtcgcc aggttcttaa ttaactgctg
    14521 agaaaccacg cgatccatct caatcaacgt ggcccccgca gcgtagcgcg ataacgcttc
    14581 cagccccagc gcgccgcttc cggcgaagca atccagacat tgggcgtcaa caatgaccgg
    14641 agccagccag ttaaacaacg tttcgcgtac gcgatcggtg gtggggcgca gacctgggct
    14701 atcaggaacc gggagtttac ggcctcgcca ctgcccgcca ataatgcgga tttggccgct
    14761 gccggaatga ttcggttttt tcatgataaa ttgctcaatc cgccagataa cacatacttg
    14821 caggcggtga tgtgaattaa gttaagtgat agactatttc atcatttttt tagctgctat
    14881 gtacatagcg ttaacgctgt gccatgaagc aacagcgagg agtgtagtcg caaatggcga
    14941 aagaaaaaaa acgtggcttt ttttcctggc tgggctttgg tcaaaaagag cagaccccgg
    15001 aaaaagagac agaagttcag aatgaacaac cggttgtaga agaaatcgtt ccggcgcaag
    15061 agcctgcgaa ggcttctgaa cacgccgttg aagagcagcc gcaggcgcat actgaagccg
    15121 aggcggaaac ttttgctgcc gacgttgtgg aagtcactga acaggttgct gaaagtgaaa
    15181 aagcgcagcc tgaagcggaa gttgttgcac agccggaacc ggtcgtagaa gaaacgccgg
    15241 agccagtagc tatcgagcgt gaagagctgc cgttgccgga agacgtcaac gccgaagagg
    15301 tttcgccaga agagtggcag gccgaagcgg aaaccgtaga gattgtcgaa gcggcggaag
    15361 aagaagcggc taaagaagaa atcaccgatg aagagctgga agcacaggcg ctggctgccg
    15421 aagcggcaga agaggcggtg atggtggttc ctccggcaga agaagagcag ccggtggaag
    15481 aaatcgctca ggagcaggaa aaaccgacca aagaaggttt cttcgcgcgc ctgaaacgca
    15541 gcctgttaaa aaccaaagaa aatcttggtt ccggatttat cagcctgttc cgcggtaaaa
    15601 aaatcgacga tgatctgttt gaagagctgg aagaacagct gttgatcgcc gatgtgggcg
    15661 tggaaaccac acgtaaaatt atcaccaatc tgactgaagg cgcatcccgc aagcagcttc
    15721 gtgatgccga ggcgctctat ggcctgctga aagaagagat gggcgagatt ctggcgaaag
    15781 tcgatgagcc gctgaatgtt gaaggtaaaa cgccgttcgt gatcctgatg gtgggcgtca
    15841 acggtgtggg taaaaccacg acgattggta agctggcacg tcagtttgag cagcagggta
    15901 aatcggtgat gctggcggcg ggcgatactt tccgtgcggc agcggttgaa cagcttcagg
    15961 tctggggtca gcgcaacaat attccggtga ttgcccagca taccggtgcg gattccgcct
    16021 ctgttatctt cgacgccatt caggccgcta aagcgcgtaa tatcgacgtc ctgattgccg
    16081 ataccgccgg acgcctgcag aacaaatcgc acctgatgga agagttgaag aaaatcgtcc
    16141 gcgtgatgaa gaaactcgac gttgaagcgc cgcatgaagt tatgctgact atcgatgcca
    16201 gcaccgggca gaacgcggta agccaggcca aactgttcca tgaagccgtt ggcttaaccg
    16261 gcatcacgct aacgaaactg gacggcacgg cgaaaggcgg ggtaattttc tcggtggctg
    16321 accagtttgg tatccctatc cgctacattg gtgtcggcga acgtattgag gatttgcgtc
    16381 cgtttaaggc ggacgacttt atagaggcac tttttgcccg agaggattaa caatgattcg
    16441 ctttgaacat gtcagcaagg cttatctcgg tgggagacag gcgctgcagg gcgttacgtt
    16501 ccatatgcag ccgggtgaga tggcgtttct gaccggtcat tccggcgcag ggaaaagtac
    16561 cctcctgaag ctgatctgtg ggattgagcg gcccagcgcc gggaaaatct ggtttagcgg
    16621 ccatgacatc acgcgtctga aaaaccgtga agttccgttt ctgcgccgcc agattggcat
    16681 gattttccag gatcaccatc tactgatgga ccgtactgtc tacgataacg tggcgatccc
    16741 gctgattatc gccggtgcca gcggtgacga tattcgtcgc cgggtgtcgg cggcactgga
    16801 taaagtcggg ctactggaca aagcgaagaa ctttcctatt cagctttccg gcggtgaaca
    16861 acagcgtgtt ggcattgccc gcgcggtggt gaacaagccc gcggtactgc tggcggatga
    16921 accgactggt aaccttgacg acgcgttgtc agaagggatt ttacgtctgt ttgaagagtt
    16981 taaccgcgtt ggggtaaccg tattgatggc aacgcacgac atcaacctga tctcgcggcg
    17041 ttcctatcgc atgctcaccc tgagcgatgg tcacttgcat ggaggcgtgg gccatgaata
    17101 agcgcgatgc aatcaatcat attcggcagt ttggcgggcg tctggatcgc ttccgtaaat
    17161 cggtcggtgg ctctggcgac ggcggtcgta acgcaccgaa acgcgcgaaa tcctcgccaa
    17221 aaccggtaaa tcgcaaaacc aacgttttca acgaacaggt gcgctatgcc ttccacggcg
    17281 cattgcaaga tctgaaaagt aaaccgttcg ccacgttttt aacggtgatg gttatcgcca
    17341 tctccctgac gctgcccagc gtctgttata tggtgtacaa aaacgttaac caggcggcga
    17401 cgcagtatta tccgtcaccg caaatcactg tttatctgca aaaaacgctg gatgatgacg
    17461 ccgctgcggg tgtggtggca cagttacagg ccgagcaagg cgtagagaaa gtgaattatc
    17521 tttctcgtga agacgcactg ggcgagttcc gtaactggtc tggttttggt ggtgcgctgg
    17581 atatgctgga agaaaacccg cttccggcag tggcggtggt gatcccgaaa ctcgattttc
    17641 aggggacaga atcactgaat acgctgcgtg atcgtatcac ccagatcaac ggcattgacg
    17701 aagtgcggat ggatgacagc tggtttgccc gtctggcggc gttaaccggg ctggtcgggc
    17761 gcgtttccgc gatgatcggc gtgttgatgg tggcagcagt gtttctcgtc atcggcaaca
    17821 gtgtgcgtct gagcatcttt gctcgccgtg actccattaa cgtgcagaaa ctgattggtg
    17881 cgacggatgg tttcatcctg cgcccgttcc tgtacggtgg cgcactgctg ggattttctg
    17941 gcgcattgct gtcattaatt ttgtcagaaa ttctggtact gcgtttgtca tcggcggttg
    18001 cggaagtggc acaggttttc ggaacaaagt ttgatatcaa tggcttatcg ttcgatgaat
    18061 gcctgctgtt gctgctggta tgctcgatga ttggctgggt ggcagcgtgg cttgccacgg
    18121 tacaacattt acgccacttt acgcctgaat aataaaagcg tgttatactc tttccctgca
    18181 atgggttccg tagcagggaa agagaccccg ttgtctcttc ccggtatttc atctctatgt
    18241 cacattttgt gcgtaattta ttcacagcgt tgcattgaac ttgtggataa aatcactgtc
    18301 tgataaaaca gtgaatgata acctcgttgc tcttaagctc tggcacagtt gttgctacca
    18361 ctgaagcgcc agaagatatc gattgagagg atttgaatga ctgacaaaat gcaaagttta
    18421 gctttagccc cagttggcaa cctggattcc tacatccggg cagctaacgc gtggccgatg
    18481 ttgtcggctg acgaggagcg ggcgctggct gaaaagctgc attaccatgg cgatctggaa
    18541 gcagctaaaa cgctgatcct gtctcacctg cggtttgttg ttcatattgc tcgtaattat
    18601 gcgggctatg gcctgccaca ggcggatttg attcaggaag gtaacatcgg cctgatgaaa
    18661 gcagtgcgcc gttttaaccc ggaagtgggt gtgcgcctgg tctccttcgc cgtccactgg
    18721 atcaaagcag agatccacga atacgttctg cgtaactggc gtatcgtcaa agttgcgacc
    18781 accaaagcgc agcgcaaact gttcttcaac ctgcgtaaaa ccaagcagcg tctgggctgg
    18841 tttaaccagg atgaagttga aatggtggcc cgtgaactgg gcgtaaccag caaagacgta
    18901 cgtgagatgg agtcgcgtat ggcggcacag gacatgacct ttgacctgtc ttccgacgac
    18961 gattccgata gccagccgat ggctccggtg ctctatctgc aggataaatc atctaacttt
    19021 gctgacggca ttgaagatga taactgggaa gagcaggcgg caaaccgtct gaccgacgcg
    19081 atgcaaggtc tggacgagcg tagccaggat atcatccgcg cgcgctggct ggacgaagac
    19141 aacaagtcca cgttgcagga actggctgac cgttacggtg tttccgctga gcgtgtgcgc
    19201 cagctggaaa agaacgcgat gaaaaaactt cgcgctgcca ttgaagcgta atttccgcta
    19261 ctcagcagag aaccctggat gagaatccgg ggtttttgtt ttttggacct ctgcaataat
    19321 cagtttcccc ctccggcaaa acgccaatcc ccacgcagat tgttaataaa ctgtcaaaat
    19381 agctattcca atatcataaa aatggggtat gttttagcag agtatgctgc taaagcacgg
    19441 gtagtcatgc ataaaacgaa ataaagtgct gaatcacaac accacaacac acgtaataac
    19501 cagaagaatg gggattctca ggatgaatat aaagggtaaa gcgttactgg caggatgtat
    19561 cgcgctggca ttcagcaata tggctctggc agaagatatt aaagtcgcgg tcgtgggcgc
    19621 gatgtctggt ccggtggccc agtacggtga ccaggagttc actggcgcag agcaggcagt
    19681 agcggatatc aatgctaaag gcggcattaa aggcaacaaa ctgcaaatcg taaaatatga
    19741 cgatgcctgt gacccgaaac aggcggttgc ggtggcgaac aaagtcgtta acgacggcat
    19801 taaatatgtg attggtcacc tctgttcttc atcaacgcag ccagcgtcag acatctatga
    19861 agatgaaggc attttaatga tcaccccagc ggcaaccgcg ccggagctga ccgcccgtgg
    19921 ctatcagctg atcctgcgca ctaccggcct ggactccgac caggggccga cggcggcgaa
    19981 atatattctt gagaaagtga aaccgcagcg tattgctatc gtccacgaca aacagcagta
    20041 cggcgaaggt ctggcgcgtg cggtgcagga cggcctgaag aaaggcaatg ctaacgtggt
    20101 gttctttgat ggtatcaccg ctggggaaaa agatttctca acgctggtgg cgcgtctgaa
    20161 aaaagagaat atcgacttcg tttactacgg cggttatcac ccggaaatgg ggcaaatcct
    20221 gcgtcaggca cgcgcggcag ggctgaaaac tcagtttatg gggccggaag gcgtggctaa
    20281 cgtctcgctg tctaacattg cgggtgaatc agcggaagga ctgctggtga ccaagccgaa
    20341 gaactacgat caggttccgg cgaacaaacc cattgttgat gcgatcaaag cgaaaaaaca
    20401 ggacccaagc ggcgcattcg tctggaccac ctacgccgcg ctgcaatctt tgcaggcggg
    20461 tctgaatcag tctgacgatc cggctgaaat cgccaaatac ctgaaagcga actccgtgga
    20521 tacggtgatg gggccgctga cctgggatga gaaaggcgat ctgaaaggct ttgagttcgg
    20581 cgtatttgac tggcacgcca acggcacggc gaccgatgcg aagtaatcat taatcggcaa
    20641 ctttgggttg ccgccaaatt gctaatatcg agtacgttgc ttcatgccgg atgcggcgta
    20701 aacgccttat ccggcctaca agatccaaag aaatcagtaa attgcagcac acattgtagg
    20761 cctgataagc gtagcgcatc aggcaatttc tcgttggtcg tcacttttga aatcaaactt
    20821 gacgattaac gcttctccca gccgccctgt tgtgccgtaa atcccagagc ctgcataaac
    20881 gctgtcatca cgccgcgatc ttccatgcct gcatccgcca tccaccagca tgaaacgcca
    20941 ggattgttac gcaaaacctc ttccagcaga tattgcccca caccgcgacg gcgggtgact
    21001 tcccgcacgc gcagggaatc cagtgcgccc tccgtgccgc ttaaggttac ccgcacggcc
    21061 gcgagaaggc gctcgttaaa acgcgcggcg tagatacggt ggttatcgtc aacctgtaaa
    21121 gaggaagggg aatactccgg ccagatcttt tgcaggtcaa tccggtcctg gtcgctaaag
    21181 ttttctaatc gaatgatggt cagcttcatg gggaacccgt gtaaatcaca aaagtggaac
    21241 cagtgtagcg aaataattta atcggaggct ttctcttttt tatttctttt ggcaggtgat
    21301 taatttttta acagcaataa ttacaaagtt aaaacattag agaatgaaaa atacccagca
    21361 taatcccctg aatggtagtg aactattccg ccctttgtgc cgttatttta tgctgacaac
    21421 ggcacttttt tctgtttatc tatcaataaa tgcagaatat tatctgttct taatcgactg
    21481 aaaaataggg attttaatcg ctattatcac aaaatactgc gctaaaccct taatcagaca
    21541 ggcaaaaaca gtgcagtata aaaaaagaac agtctgattt gttaacacat aaaaacaaag
    21601 caacacaaca tcacgaatgg ggatttttga ctatgaaacg gaatgcgaaa cctatcatcg
    21661 cagggatgat tgcactggca atttcacaca ccgctatggc tgacgatatt aaagtcgccg
    21721 ttgtcggcgc gatgtccggc ccgattgccc agtggggcga tatggaattt aacggcgcgc
    21781 gtcaggcgat taaagacatt aatgccaaag ggggaattaa aggcgataag ctggttggcg
    21841 tggaatatga cgacgcctgc gacccgaaac aagccgttgc ggtcgccaac aaaatcgtta
    21901 acgacggcat taaatacgtt attgggcact tgtgttcctc ctctacccaa cctgcatcag
    21961 atatctacga agacgaaggt attctgatga tctcgccggg agcgaccaac ccggagctga
    22021 cccaacgcgg ttatcaacac atcatgcgca ctgccgggct ggactcttcc caggggccaa
    22081 cggcggcaaa atacattctt gagacggtga agccccagcg catcgccatc attcacgaca
    22141 aacaacagta tggcgaaggg ctggcacgtt cggtgcagga cgggctgaaa gcggctaacg
    22201 ccaacgttgt cttcttcgac ggtattaccg cgggtgagaa agatttctcc gcgctgatcg
    22261 cccgcctgaa aaaagaaaat atcgacttcg tttactacgg cggttactac ccggaaatgg
    22321 ggcagatgct gcgccaggcc cgttccgttg gcctgaaaac ccagtttatg gggccggaag
    22381 gtgtgggtaa tgcgtcgttg tcgaatattg ctggcgatgc tgccgaaggc atgttggtca
    22441 ctatgccaaa acgctatgac caggatccgg caaatcaggg catcgttgat gcgctgaaag
    22501 cagacaagaa agatccgtcc gggccatatg tctggatcac ttacgcggcg gtgcaatctc
    22561 tggcaacagc ccttgagcgt accggcagcg atgagccgct ggcgctggtg aaagatttaa
    22621 aagctaacgg tgcaaacacc gtgattgggc cgctgaactg ggatgaaaaa ggcgatctta
    22681 agggatttga ttttggtgtc ttccagtggc acgccgacgg ttcatccacg gcagccaagt
    22741 gatcatccca ccgcccgtga aaaacgggcg ggtttagaaa ggttacctta tgtctgagca
    22801 gtttttgtat ttcttgcagc agatgtttaa cggcgtcacg ctgggcagta cctacgcgct
    22861 gatagccatc ggctacacca tggtttacgg cattatcggc atgatcaact tcgcccacgg
    22921 cgaggtttac atgattggca gctacgtctc ctttatgatc atcgctgcac tgatgatgct
    22981 gggcattgat accggctggt tgctggtggc ggcgggattc gtcggcgcaa tcgtcattgc
    23041 cagcgcctac ggctggagta tcgaacgggt ggcttaccgc ccggtgcgta actctaagcg
    23101 cctgattgcg cttatttccg caatcggcat gtctatcttc ctgcaaaact acgttagcct
    23161 gaccgaaggt tcgcgcgacg tggcgctgcc cagcctgttt aacggtcagt gggtggtggg
    23221 ccatagcgaa aacttctctg cctctattac caccatgcag gcggtgatat ggattgttac
    23281 tttcctcgcc atgctggcgc tgacgatttt cattcgttat tcccgtatgg ggcgcgcctg
    23341 ccgtgcctgc gcggaagatc tgaaaatggc gagcctgctt ggcatcaaca ccgatcgggt
    23401 gattgcgctg acctttgtga ttggcgcggc gatggcggcg gtggcgggtg tgctgctcgg
    23461 tcagttctac ggcgtcatta acccctacat cggctttatg gccgggatga aagcctttac
    23521 cgcagcggtg ctcggtggga ttggcagcat tccgggggcg atgattggcg gcctgatcct
    23581 ggggattgcg gaggcgctct cttctgccta tctgagtacg gaatataaag atgtggtctc
    23641 attcgccctg ctgattctgg tgctgctggt gatgccgacc ggtattctgg gtcgcccgga
    23701 ggtagagaaa gtatgaaacc gatgcatatt gcaatggcgc tgctctctgc tgtgatgttc
    23761 tttgtgctgg cgggcgtctt tatgggcgtg caactcgagc tggatggcac caaactggtg
    23821 gtcgatagcg cttcagacgt ccgttgggaa tgggtgttta tcggcacggc ggtggtcttt
    23881 ttcttccagc ttttgcgacc ggctttccag aaagggctaa aaagcgtttc cgggccgaag
    23941 tttattctgc cagcgattga tggctccacg gtgaagcaaa agctgtttct cgtggcgctg
    24001 ctggtgcttg cggtggcgtg gccgtttatg gtttcacgtg ggacggtgga tatcgccacc
    24061 ctgaccatga tctacattat tctcggtctc gggctgaacg tggtggtagg tctttctggt
    24121 ctgctggtgc tgggatacgg cggcttttac gccatcggcg cttacacttt tgcgctgctc
    24181 aatcactatt acggtctggg cttctggacc tgtctgccga ttgccgggtt gatggcggcg
    24241 gcggcgggct tcctgctcgg cttcccggtg ttgcgtctgc gcggtgacta tctggcgatc
    24301 gttaccctcg gtttcggcga aattgtgcgc atattgctgc tcaataacac cgaaattacc
    24361 ggcggaccga acggtatcag tcagatcccg aaaccgacgc tcttcggact cgagttcagc
    24421 cgtaccgccc gtgaaggcgg ctgggacacg ttcagtaatt tctttggcct gaaatacgat
    24481 ccctccgatc gcgttatctt cctgtacctg gtggcgttgc tgctagtggt gttaagcctg
    24541 tttgtcatta accgcctgct gcggatgccg ctggggcgtg cgtgggaagc gttgcgtgaa
    24601 gatgaaatcg cttgtcgttc gctgggctta agcccgcgtc gtatcaagct gactgccttt
    24661 accatcagcg ctgcgtttgc cggttttgcc ggaacgctgt ttgctgcgcg tcagggcttt
    24721 gtcagcccgg aatccttcac ctttgctgaa tcagcctttg tactggcgat agtggtgctc
    24781 ggcggtatgg gctcgcaatt tgcagtgatt ctggcggctg ttctgctggt ggtatcgcgc
    24841 gagttgatgc gtgatttcaa cgaatacagc atgttaatgc tcggtggttt gatggtgctg
    24901 atgatgatct ggcgtccgca gggcttgctg cccatgacgc gcccgcaact gaagctgaaa
    24961 aacggcgcag cgaaaggaga gcaggcatga gtcagccatt attatccgtt aacggcctga
    25021 tgatgcgctt cggcggcctg ctggcggtga acaacgtcaa tctcgaattg tacccgcagg
    25081 agatcgtctc gttaatcggc cctaacggtg ccggaaaaac cacggtcttc aactgcctga
    25141 ccggattcta caaacccacc ggcggtacca ttttactgcg cgatcagcac ctggaaggtt
    25201 taccggggca gcaaatcgcc cgcatgggcg tggtgcgcac cttccagcat gtgcgtctgt
    25261 tccgtgaaat gacggtgatt gaaaacctgc tggtggcgca gcatcagcaa ctgaaaaccg
    25321 ggctgttctc cggcctgttg aaaaccccct ctttccgtcg cgcccagagt gaagcactcg
    25381 accgcgccgc cacctggctt gagcgcattg gtttgctgga acacgccaac cgtcaggcga
    25441 gtaacctggc ctatggtgac cagcgccgtc tggagattgc ccgctgtatg gtgacgcagc
    25501 cggagatttt aatgctcgat gaaccggcgg cgggtcttaa cccaaaagag acgaaagagc
    25561 tggatgagct gattgccgaa ctgcgtaacc atcacaacac cactatcttg ttgattgaac
    25621 acgatatgaa gctggtgatg gggatttcag accgaattta cgtggtcaac caggggacgc
    25681 cgctggcaaa cggtacgccg gagcagatcc gtaataaccc ggacgtgatc cgtgcctatt
    25741 taggtgaggc ataagatgga aaaagtcatg ttgtcctttg acaaagtcag cgcccactac
    25801 ggcaaaatcc aggcgctgca tgaggtgagc ctgcatatca atgagggcga gattgtcacc
    25861 ctcattggcg cgaacggcgc ggggaaaacc accttgctcg gcacattatg cggcgacccg
    25921 cgcgccaccg gcgggcgaat tgtgtttgat gataaagaca ttaccgactg gcagacggcg
    25981 aaaatcatgc gcgaagcggt ggcgattgtc ccggaagggc gtcgtgtttt ctcgcggatg
    26041 acggtggaag agaacctggc gatgggcggc ttttttgccg aacgcgacca gttccaggag
    26101 cgcataaagt gggtctatga actgtttccg cgtctccatg agcgccgtgt tcagcgagcg
    26161 ggcactatgt ctggcggtga acagcagatg ctggcgattg gtcgcgcttt gatgagtaac
    26221 ccgcgtttgc tactgcttga tgagccatcg ctcgggctgg caccgattat catccagcaa
    26281 attttcgaca ccatcgaaca actgcgcgag caggggatga ccatcttcct cgtcgagcag
    26341 aacgccaacc aggcgctaaa gctcgctgat cgcggctacg tgctggaaaa cggtcatgtg
    26401 gtgctctccg ataccggcga tgcgctgctg gcaaacgaag cggtgagaag tgcgtattta
    26461 ggcgggtaat aacacgttga ttgataagga gtcaaaagac tcctttgaga caggttacaa
    26521 atgtaaaatt gcctgatgcg ctgcgcttat caggcctact gggtgagttg caatatattg
    26581 aatttgcacg atcttgtagg ccggataagg cgtttacgcc gcatccggca tgaaacgatg
    26641 agcaatctgt agagtttgat tcagaccttc tatttttccc gcttatccgt gccccatctc
    26701 ccattttccc tcacccgtac cgtcaccgcc ttgtcatctt tctgacacct tactatctta
    26761 caaatgtaac aaaaaagtta tttttctgta attcgagcat gtcatgttac cccgcgagca
    26821 taaaacgcgt gaattcgcgc attcggtaca acaagagaga taaacgatga aaccgttaca
    26881 ttatacagct tcagcactgg cgctcggact ggcgttaatg gggaatgcac aggcagtgac
    26941 gaccattccg ttctggcatt ctatggaagg ggaactgggt aaagaggtgg attctctggc
    27001 ccaacgtttt aacgccgaaa acccggatta caaaattgta ccgacctata aaggcaacta
    27061 cgaacagaat ttaagcgcgg ggattgccgc atttcgtacc ggcaacgcgc ctgctatttt
    27121 gcaggtttat gaagttggca ccgccaccat gatggcgtcg aaagccatta aaccagtata
    27181 tgacgtgttt aaagaggcgg ggattcagtt cgatgagtcg cagtttgtgc cgacggtttc
    27241 aggttactac tccgacagca aaaccggcca cttactctcc cagccgttca acagctcgac
    27301 tcccgttctc tattacaaca aagacgcctt caagaaagcc ggtttagacc cggaacagcc
    27361 gccgaaaacc tggcaggatc tggcggacta tgccgcgaaa ctgaaagcct ccggtatgaa
    27421 gtgcggctac gccagcggct ggcagggctg gatccaactg gaaaacttta gcgcctggaa
    27481 cggtctgccg tttgccagca aaaacaacgg ctttgacggc acagacgcgg tgctggagtt
    27541 caacaagccg gagcaggtga aacacatcac tatgctcgaa gagatgaaca agaagggcga
    27601 tttcagctac gtcgggcgta aggatgaatc cactgagaag ttctataacg gtgattgcgc
    27661 gatgaccact gcctcttccg gttctcttgc caacattcgc gagtacgcca aatttaacta
    27721 cggcgtaggc atgatgcctt acgacgccga tgcgaaagat gcaccgcaaa atgccattat
    27781 cggtggagcc agcctgtggg tgatgcaagg caaagataaa gaaacttata cgggtgtggc
    27841 gaagttcctc gacttcctcg cgaaaccaga aaacgctgct gagtggcatc agaaaaccgg
    27901 ttatctgcca atcaccaaag cggcctatga cctgacgcgt gagcagggct tttatgagaa
    27961 aaacccaggg gcggataccg cgacgcgtca gatgctgaac aagccgccgt tgccgttcac
    28021 caaagggctg cgtctgggca acatgccgca aatccgcgtg attgtggatg aagagctgga
    28081 gagcgtgtgg accggtaaga agacgccaca gcaggcgctg gataccgccg ttgagcgtgg
    28141 aaatcagttg ctgcgccgct ttgagaaatc gacgaagtct taatcagtgt aatgtcggat
    28201 gcgtttcgct tatctgacct ggcatcgcgt gtaggccgga taagcgaagc gcatccggca
    28261 cagttcagga attaaccgta atgtcatcat cccgtccggt gtttcgctcg cgctggctgc
    28321 cttatttgct ggtcgcgccg cagctcatca tcaccgttat cttttttatc tggcctgcgg
    28381 gcgaagcgtt gtggtactcg ctacaaagcg tcgatccgtt tggtttctcc agccagtttg
    28441 tcggcctgga taacttcgtc acgttgtttc atgacagcta ctatctcgac tccttctgga
    28501 cgacgataaa attcagcacc tttgtcaccg tcagcggttt gctggtatcg ctgttcttcg
    28561 cagcgctggt ggagtacatc gtgcgcggca gccgtttcta tcaaacctta atgctgctac
    28621 cgtatgctgt ggctcccgcc gttgccgccg tattatggat cttcctgttt aaccccggtc
    28681 gcgggctgat cacccatttt ctcgctgagt tcggctatga ctggaaccac gcacaaaaca
    28741 gcggtcaggc gatgttcctg gtggtgtttg cctcggtgtg gaagcaaatt agctacaact
    28801 tcctgttctt ctatgctgcg ctgcaatcca ttccccgttc gttgatcgaa gccgcagcca
    28861 tcgacggtgc agggccgatt cgccgcttct ttaagattgc gctgccgctt atcgctccgg
    28921 tgagtttctt cctgctggtg gtgaacctgg tatacgcctt cttcgacacc ttcccggtga
    28981 tcgacgccgc cacgtccggc gggccggttc aggccaccac gacgctgatt tataagatct
    29041 accgcgaagg ctttaccgga ctggatctgg cttcgtctgc cgcgcagtcg gtagtgttga
    29101 tgttcctcgt catcgtgctg acggtggtgc agttccgcta tgttgaaagc aaggtgcgtt
    29161 accaatgatt gagaaccgtc cgtggctgac aatattcagc cataccatgc tgatcctcgg
    29221 gatcgcggtg atcctcttcc cgctgtacgt ggcgtttgtc gcggcgacgc tggataaaca
    29281 ggccgtctac gccacgccga tgacgctcat ccccggccca catctgctgg aaaacatcca
    29341 caacatctgg gtgaacgggg taggcacgaa tagtgcgcct ttctggcgga tgttgcttaa
    29401 cagctttgtg atggcgttca gcattacgct cggcaaaatt accgtctcga tgctctcggc
    29461 atttgccatt gtctggtttc gttttccgct gcgtaacctc ttcttctgga tgatttttat
    29521 cactctgatg ttgccggttg aagtgcgtat tttcccgacg gtggaagtca tcgccaatct
    29581 gaagatgctc gacagctacg ccggcttaac gctgccgctg atggcctcgg cgaccgccac
    29641 cttcctgttc cgccagttct ttatgacgct gccggatgag ctggtggaag ccgcgcggat
    29701 cgacggcgcg tcaccaatgc gtttcttttg cgacatcgtt tttccgctgt ccaaaaccaa
    29761 tctggcggcg ctgtttgtga tcacctttat ctacggctgg aaccagtatt tgtggccgtt
    29821 gttgattatt accgatgtgg atctcggcac caccgtagca gggattaaag ggatgatcgc
    29881 cacaggcgaa ggcaccacgg aatggaactc ggtgatggcg gcgatgttgt taacgctgat
    29941 ccctccggtg gtgattgttt tagtgatgca gcgtgccttt gtgcgcggcc tggtcgatag
    30001 tgagaaataa gatggcagga ctgaaattac aggcagtaac caaaagctgg gatggcaaaa
    30061 cccaggtcat taaaccgctg acccttgatg tggcggatgg cgaatttatc gtgatggtcg
    30121 ggccgtctgg ctgcgggaaa tcgacgttat tgcgaatggt ggccggactg gagcgggtaa
    30181 cgacgggcga tatctggatc gaccgcaagc gtgtcaccga aatggagccg aaagatcgcg
    30241 ggattgcgat ggtgttccag aactacgcgc tctatccgca tatgagcgta gaggagaata
    30301 tggcgtgggg gctgaaaatt cgcggcatgg gcaagcaaca aattgccgaa cgtgttaaag
    30361 aagcggcgcg cattctggag ctggacggcc tgcttaagcg ccgcccgcgt gagctatctg
    30421 gcggtcagcg tcagcgtgtg gcgatggggc gtgcgatcgt gcgtgagccg gcagtgttcc
    30481 tgtttgatga gccgctctct aacctcgatg ccaagttgcg tgtgcagatg cgtcttgaac
    30541 tgcaacagct gcaccgtcgc ctgaaaacga cttcactcta cgttacccac gatcaggttg
    30601 aggcgatgac gctcgcccag cgagtaatgg tgatgaacgg cggcgttgcc gaacagattg
    30661 gcacgccagt tgaagtgtac gaaaagcccg ccagcctgtt tgtggcgagt tttatcggca
    30721 gtccggcgat gaatctgctg gcagggcgtg tgaataacga aggtacgcat ttcgaactgg
    30781 acggcggtat cgcgctgccg ctaaacggtg gctaccgtca gtatgctgga cgtaaaatga
    30841 ctctcggcat tcgcccggaa catattacgc tacgttcgca ggctgaaggt ggcgtgccgt
    30901 tggtgatgga cacgctggag atcctcggcg ctgataatct ggcgcacgga cgctggggcg
    30961 aacagaagct ggtggtgcga ctggcgcatc aggagcgccc gacggcaggc agcacgctgt
    31021 ggctgcatct gccggaaaat cagctacatc tttttgatgg tgaaacagga caacgagtat
    31081 gagtaactgg ccttatcccc gcatcgtcgc ccatcgtggc ggcggtaaac tggccccgga
    31141 gaacaccctg gcggcaatcg acgtgggggc aaaatacggt cataagatga tcgaatttga
    31201 cgcgaagtta tcgaaggatg gcgagatctt cttgctccat gacgacaatc tcgaacgcac
    31261 cagcaacggc tggggcgtcg cgggtgaact gaactggcag gatttactgc gcgtggatgc
    31321 gggcagttgg tacagcaaag cgtttaaagg tgagccgctg ccgttgcttt cgcaggtggc
    31381 ggaacgctgt cgtgaacacg ggatgatggc gaatatcgaa atcaagccca ccactggcac
    31441 cgggccatta acgggcaaaa tggtggcgct ggctgcacgc gaactgtggg ctggtatgac
    31501 gtcgccgctg ctgtcatcgt ttgagattga tgctttagaa gccgcgcaac aagcagtacc
    31561 agaactgccg cgcggtttat tgcttgatga gtggcgcgac gactggcgag aactgaccgc
    31621 gcggctgggc tgcgtctcta ttcatctcaa tcataagttg ctcgataaag cgcgagtgat
    31681 gcagttgaaa gacgccggat tacggattct ggtttatacc gtcaataaac cccagcgcgc
    31741 ggcagagttg ctgcgctggg gcgtggattg catctgtacc gatgcgattg atgtgattgg
    31801 tccgaacttt acggcccaat agttttcaac ggtatatcag gctgcggcgt gccgggctga
    31861 ttcagcatgt cgccgtttct cctctccggc aacatatgct gctgattcaa cgaactatcc
    31921 ggattacggt tgctgtttaa catcccgcca ttggtgttgg gcagcatttg ctgccgggcg
    31981 ggatttcgct cccccggctg tgactgcaac acccgctgag aattattgtt tatctggttt
    32041 tctaaatgct gttgttgcag ctgcgtttgc gttttcagtt gctgattcag catccctttt
    32101 tgctggattt gctgcgtttg catttgggtt tgcatccgct gctggctggg gatctgatat
    32161 cccggctggt tggggttgtt cagagtatta atgggctgtg caaagccgac aaacggcagg
    32221 agtgccgtaa gaatcagaag tcgtttcatc gcgtatcctc ctctgaagat atcctttaag
    32281 tttactcgct tcccggcaaa acgatgatta attcagagtt atataccagg cttagctggg
    32341 gttgcccctt aatctctgga gaataacgat gataaaaccg acgtttttac gccgggtggc
    32401 cattgctgct ctgctctcag gaagttgttt tagcgccgcc gccgcgcctc ctccgccacc
    32461 tgtttcttat ggcgtggagg aagatgtctt ccatccggta cgcgcgaaac aggggatggt
    32521 ggcgtctgta gatgctactg ccactcaggt gggggtggat atcctcaagg agggcgggaa
    32581 tgccgttgat gccgccgtgg ctgtgggcta cgcgctggcg gtaacgcatc cgcaggcagg
    32641 gaatctgggc ggtggtggtt ttatgctgat ccgctcgaaa aatggcaata ccaccgctat
    32701 cgatttccgc gaaatggcac ccgccaaagc gacccgcaat atgttcctcg atgaccaggg
    32761 caacccggac agcaaaaaat cactcacttc gcatctggcc tccggtacgc caggtacggt
    32821 cgcaggtttc tcgctggcgc tggagaagta cggcaccatg ccgttaaata aagtggtgca
    32881 gccggcgttt aaactggcac gcgatggttt tatcgttaat gacgcgctgg ctgacgatct
    32941 caaaacctac ggtagcgaag tgttgccgaa tcacgaaaac agcaaggcta tcttctggaa
    33001 agagggcgcg ccgctgaaaa agggcgacaa gctggtgcag gcgaacctgg caaagagcct
    33061 ggagatgatt gccgaaaacg gcccggacga attctacaaa ggcacaatag cggagcagat
    33121 cgctcaggag atgcagaaaa acggtggctt gatcactaaa gaagatttag cggcgtataa
    33181 agcggtcgaa cgtacaccga ttagcggcga atatcgcggg tatcaggttt actcgatgcc
    33241 accgccatct tccggtggga ttcatatcgt gcagatcctc aatatcctgg aaaacttcga
    33301 tatgcagaaa tacggctttg gcagcgccga tgcgatgcaa atcatggcag aagcggagaa
    33361 atacgcctac gccgaccgct cggaatatct tggcgatccg gattttgtga aagtcccgtg
    33421 gcaggcgctg accaataaag cctatgccaa atctattgcc gatcaaattg atatcaataa
    33481 agcgaagcca tccagcgaaa ttcgccccgg caagcttgcg ccttatgaga gtaatcaaac
    33541 tacccattac tcagtggtgg ataaagatgg taacgcggtg gcggtgacct atacgctgaa
    33601 caccaccttc ggtacgggca ttgtcgcggg cgagagcggt attctgctta ataaccagat
    33661 ggatgatttc tccgccaaac cgggcgtacc gaacgtttac gggctggtgg gcggtgatgc
    33721 caacgccgtc gggccgaata aacgcccgct gtcgtcgatg tcaccaacca ttgtggcgaa
    33781 agacggtaaa acctggttag tgactggtag cccaggcggt agccggatca tcactaccgt
    33841 gctgcaaatg gtagtgaata gtatcgatta tggcatgaac gtcgccgaag cgaccaatgc
    33901 gccgcgtttt caccatcagt ggttgccgga cgagctgcgt gtcgaaaaag ggtttagccc
    33961 ggatacgatc aagctgctgg aagcaaaagg tcagaaagtg gcgctgaaag aggcgatggg
    34021 cagtacacaa agcattatgg ttgggccgga cggcgagttg tatggggcat ccgacccgcg
    34081 ctcggtggat gatttaacgg cgggttatta aggttagcgg ccctcttcgt gggaagaggg
    34141 ccgtattgtc agggcaagcc gaaggtagcc ttttttattt cataatcctg tagatattct
    34201 tccagcggct ttgccgttcc aagggaatga atttcaccac tgtctttatc aataataaaa
    34261 ggtgcgttac cggctaagcg cgcggcctca tcaccagatt caagaaattc ttgtgattcg
    34321 aaacagaaat accagccctg gctaaaacgt ccatgcagag tgatgacgac agggagatct
    34381 gcatcatcaa ggtaatggtt cgctttcgcg aatgcgtcgt gataagtaat cataattata
    34441 ataaatatta ttgttgaacg ttatattatt attcttgcgt tatactttga gaaaaggcgt
    34501 gttcatcttc gtcaaagata ttattaaatg ctggttttga aaaaagttca tcggataata
    34561 tacagtcacg aaataagtat ctgctaatat tttgtgattt ctttttcaga acgtcgaacg
    34621 ggattttctt atttaagata tcagaatctt atgattcggg tgaaattcta actaaatcaa
    34681 catgtcacta aaatcatcat agccattagc tatttcggct aaaatagaga ttacatgtct
    34741 tcggtccatc tcacttaagg agtgtagttc cgttgtaagt ttttccatag cttgcattgc
    34801 taagtttcga acaaggaatt ttctgctggt aatctctaaa aagatggcat ggtttacaat
    34861 gatttttgtt atcttttgat tattatgaac aactgtccat gatttcgttt aagaatgaag
    34921 agaaatcact aaacgaactg aatatatttt cggtgccaat attatctcta atttcaaaaa
    34981 agttattttt aatgtcataa gtaaatattg aaatgctatc atcaccgatc actaaccgtt
    35041 cttgtagatc agggtttatg aaatcatcat tatttctata aaattcattt acggcaaaaa
    35101 tgtttttaac tgaaggttct ggaatcgaga ggctaaataa tcgtaatcca ttagcttcga
    35161 atccatccat tgctcttaaa aaagtaagat aatcaggttg attcttaaaa agaatactaa
    35221 cttcattaca taacttctcc aggcaattag ttgaattaga attaaattct atttggatta
    35281 tactttcaat aaatgattcc ttaagcggtg cttcaaatgg atagtctaaa tcattcatca
    35341 ttttttttaa tttatttatt tgggtcttag tcatcatttt gtgggttcca taatattttt
    35401 catgggagga taaattgagc cagatggaat ggcccaggga gtgattttgc tttcacctac
    35461 taatactccc ttagctattc gtgattgcat gttagtaaaa actttatgat atggttcgtt
    35521 ttcgattaat actaaatttt cgaaatcatt agtaccacca tcatctagag atagtttatg
    35581 gtgaacatta taatttagag gaacattgcc ctttctcatt cttaaaaggt cttcggcgtt
    35641 gaatcttcca gatgcttcgg aggtttttgc aagtttgata agaaaatttt ttcttacagt
    35701 tttattaaac tcctttcgta agttagctaa gtcttttgaa ggtctcttaa tgtatattat
    35761 ttctattatg tctataccat caagaagttt ttgttgacct ctaagtttgc ctaagtaact
    35821 atttatttct gtatatgtat caacattaga acgctttgtt aaaagaatag atgctgttgc
    35881 tatcgctatg cccgctgttt tttcatttag tatttgatca tagcctttta ttgcatctcc
    35941 acccagtttt tccgcagtat ctctgaatcc ttcaatgttc ccattgtata cgccaccagc
    36001 agcaagtagc ctgccaatag ctttgctgtt taacgttttg aaacgattat ttgtttggtt
    36061 ttttgaattt ggtaaaaaca aacggttata attttcaggg agtgccaaat agctgaactc
    36121 ggtatttgcg ataagatgtt gacagaggat aaattcataa ctaacagtta ttgtttctgt
    36181 atccagttga ttttgaatga tctgatgatg aatagccgat aaaaaagcgc ctcttagttg
    36241 tatataataa tacttttccc atctaccaaa tctattgatt ctataaaagt caaattccat
    36301 aaataattgc tcattattgt taatggaatt aataagtaat ggagtgcttt tatcaattaa
    36361 tttacaaaat gtcataccct gtaactgtga accaagtccc gtattagtaa ttctatttgc
    36421 gagtgagaat gtaaatattt catcctcatg cccactctgc cagcgattac ctatagattc
    36481 agtagtccca caacctgcgg agatgtttcc ttgttgttct cccgttactg tcaggtaaac
    36541 aatattactc atccctgaaa agtcctttta tggcatgcta ttttattaat ggcactatta
    36601 tgcttgttaa ttgttattta tttactccag gaaattgtag gcgtatatat taataataaa
    36661 atattacact ttaggacgta attatgaggt aaatcttata tatttattgg gaaaataaat
    36721 acattaaagg aaggtatgcc gattttgaat gacacccttt ttccatctca gaaaaagggc
    36781 actatcttac ttcacccgcg ccatataata cgcatcgaca tattcaccat tacgtaatgc
    36841 gtacttcttg ccagtccctt caatctcaaa tccaaatttt ttatagacct taattgctgg
    36901 cgcgttatcg atgaacaccg ttagctcaat acgatctacc cgcaaccagt tgtcgcacat
    36961 ttcaatcatc tctcgcatca gggcgctggc gacgccgcga ttcttccaac gggagtcgac
    37021 acaaatacca aaatctgcaa catgactgcg gcgtgggcgc tgttgcacgt caatggtgag
    37081 atggcccacg acgtctccat caatacaggc gacgagttgc ttgatgccgg gacgatcggc
    37141 gagtcgctcc tgccacatat gatcggaagg atgaggcacc tgtagcgtgt tgcaatacac
    37201 ctccggctgg gcgtgaatct gcctgatggc ctcgtaatcg cgcgtttcag catggcgtat
    37261 cactatctca ctcattcctt tgtcctcttt ggggtaaatg tccctttcaa catcattgac
    37321 tttcaaatgt gagtcaaatg catttttttt gcaaaaagtg ttggacaagt gcgaatgaga
    37381 atgattatta ttgtcttgcg atcaggaaga ccctcgcgga gaacctgaaa gcacgacatt
    37441 gctcacattg cttccagtat tacttagcca
//
LOCUS       DAPOZO010000047        36840 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPOZO010000047 DAPOZO010000000
VERSION     DAPOZO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.047"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(5..1972)
                     /gene="aaeB"
                     /locus_tag="ROI21_002496"
     CDS             complement(5..1972)
                     /gene="aaeB"
                     /locus_tag="ROI21_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDX5111408.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAVTRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(1978..2910)
                     /gene="aaeA"
                     /locus_tag="ROI21_002497"
     CDS             complement(1978..2910)
                     /gene="aaeA"
                     /locus_tag="ROI21_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDX5111409.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(2918..3121)
                     /locus_tag="ROI21_002498"
     CDS             complement(2918..3121)
                     /locus_tag="ROI21_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDX5111410.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            3304..4233
                     /gene="aaeR"
                     /locus_tag="ROI21_002499"
     CDS             3304..4233
                     /gene="aaeR"
                     /locus_tag="ROI21_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDX5111411.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(4361..5806)
                     /gene="tldD"
                     /locus_tag="ROI21_002500"
     CDS             complement(4361..5806)
                     /gene="tldD"
                     /locus_tag="ROI21_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDX5111412.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(5962..9762)
                     /gene="yhdP"
                     /locus_tag="ROI21_002501"
     CDS             complement(5962..9762)
                     /gene="yhdP"
                     /locus_tag="ROI21_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDX5111413.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKSPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTEVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTH
                     GDSTNSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(9830..11299)
                     /gene="rng"
                     /locus_tag="ROI21_002502"
     CDS             complement(9830..11299)
                     /gene="rng"
                     /locus_tag="ROI21_002502"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDX5111414.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(11289..11882)
                     /gene="yhdE"
                     /locus_tag="ROI21_002503"
     CDS             complement(11289..11882)
                     /gene="yhdE"
                     /locus_tag="ROI21_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDX5111415.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVTHTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(11891..12379)
                     /gene="mreD"
                     /locus_tag="ROI21_002504"
     CDS             complement(11891..12379)
                     /gene="mreD"
                     /locus_tag="ROI21_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDX5111416.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(12379..13482)
                     /gene="mreC"
                     /locus_tag="ROI21_002505"
     CDS             complement(12379..13482)
                     /gene="mreC"
                     /locus_tag="ROI21_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDX5111417.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(13548..14591)
                     /gene="mreB"
                     /locus_tag="ROI21_002506"
     CDS             complement(13548..14591)
                     /gene="mreB"
                     /locus_tag="ROI21_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDX5111418.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(14896..16836)
                     /gene="csrD"
                     /locus_tag="ROI21_002507"
     CDS             complement(14896..16836)
                     /gene="csrD"
                     /locus_tag="ROI21_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDX5111419.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAVLLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCERS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLALVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16988..17962
                     /locus_tag="ROI21_002508"
     CDS             16988..17962
                     /locus_tag="ROI21_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDX5111420.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAQEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            18940..19410
                     /gene="accB"
                     /locus_tag="ROI21_002509"
                     /gene_synonym="fabE"
     CDS             18940..19410
                     /gene="accB"
                     /locus_tag="ROI21_002509"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDX5111421.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            19421..20770
                     /gene="accC"
                     /locus_tag="ROI21_002510"
     CDS             19421..20770
                     /gene="accC"
                     /locus_tag="ROI21_002510"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDX5111422.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            20879..21121
                     /locus_tag="ROI21_002511"
     CDS             20879..21121
                     /locus_tag="ROI21_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDX5111423.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            21111..22562
                     /gene="panF"
                     /locus_tag="ROI21_002512"
     CDS             21111..22562
                     /gene="panF"
                     /locus_tag="ROI21_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDX5111424.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTPVPQATVLTTDK"
     gene            22574..23455
                     /gene="prmA"
                     /locus_tag="ROI21_002513"
     CDS             22574..23455
                     /gene="prmA"
                     /locus_tag="ROI21_002513"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDX5111425.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            23784..24749
                     /gene="dusB"
                     /locus_tag="ROI21_002514"
     CDS             23784..24749
                     /gene="dusB"
                     /locus_tag="ROI21_002514"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDX5111426.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            24775..25071
                     /gene="fis"
                     /locus_tag="ROI21_002515"
     CDS             24775..25071
                     /gene="fis"
                     /locus_tag="ROI21_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDX5111427.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            25157..26041
                     /gene="yhdJ"
                     /locus_tag="ROI21_002516"
     CDS             25157..26041
                     /gene="yhdJ"
                     /locus_tag="ROI21_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDX5111428.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            26125..26304
                     /locus_tag="ROI21_002517"
     CDS             26125..26304
                     /locus_tag="ROI21_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDX5111429.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(26307..26969)
                     /gene="acrS"
                     /locus_tag="ROI21_002518"
     CDS             complement(26307..26969)
                     /gene="acrS"
                     /locus_tag="ROI21_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDX5111430.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQRGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQGCQQQGCIANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
     gene            27368..28525
                     /gene="acrE"
                     /locus_tag="ROI21_002519"
     CDS             27368..28525
                     /gene="acrE"
                     /locus_tag="ROI21_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDX5111431.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLPFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADFASK"
     gene            28537..31641
                     /gene="acrF"
                     /locus_tag="ROI21_002520"
     CDS             28537..31641
                     /gene="acrF"
                     /locus_tag="ROI21_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDX5111432.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKDATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            31894..32115
                     /locus_tag="ROI21_002521"
     CDS             31894..32115
                     /locus_tag="ROI21_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111433.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            32546..33571
                     /locus_tag="ROI21_002522"
     CDS             32546..33571
                     /locus_tag="ROI21_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX5111434.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            33639..34820
                     /locus_tag="ROI21_002523"
     CDS             33639..34820
                     /locus_tag="ROI21_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDX5111435.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            34830..35933
                     /locus_tag="ROI21_002524"
     CDS             34830..35933
                     /locus_tag="ROI21_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDX5111436.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRTDCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            35941..36699
                     /locus_tag="ROI21_002525"
     CDS             35941..36699
                     /locus_tag="ROI21_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDX5111437.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
BASE COUNT         8863 a         9529 c         9333 g         9115 t
ORIGIN      
        1 tgacttaact atcggtcaac gcatgttgat acttatgcag catccccgcc agccgatgaa
       61 ccggttccgt cacctgcggt ggcgcttgcc agatgcgtag cttttcctgg tagatttcaa
      121 gttcttccag caactggcca aagtaccgac gacgtttatc atcgctacgg gcagatatca
      181 catggtctgc tgtgcggcgc atttgtcggt gaaacgccga taaatcctcg ttaaccggga
      241 tcggcgcatc acgcaggcgc tggtgcgcga taatcatcgt cagcgccagg cgaaatttcg
      301 gcaaatcccc tgggaactta ttcatcagca aaaacagctg ctgataaagt gccgggaggt
      361 ggttctcttt acgacgtgcc acattggtag tcatcgcgga aacagcggca gaaacaaact
      421 gattaagcag tacacgtccg gtcctgtcgc gcgatttatc ccgcaccagc aaaataacgg
      481 tgaacgcgag cacacaaccg acgatttgcc ctaatgcgct gtcgagaaac tgactgaaat
      541 ggaaagtcat cgggttatcc agcacgataa tatttatggt gctggccagt gcccccatcg
      601 agcccagtcg ccgtttctgt acttctatac cgaggaagaa tcccagcact gccaggctaa
      661 tgcacagcag caacatgctc tgttgggtat tagggataat caccaaaaag tagagcagcc
      721 ctaacggcag cgctgccagc gtcccgtaga taaagtcgat cgccaccatg cgtggatttg
      781 gcaaacgcat tgccagtgac gtcactaccg caatcatcac cattgcacca ctgccggaag
      841 tccagcccgt ccacagccag aaaagcgtgc ccagaatgca ggaaagtgtg gttcgccaga
      901 agttaaccat cgcatgatga cgttcggctg actctacttt gacttccggt tcgccttgca
      961 ggatctcttc ttcggtggcg ttgatttttg tgttactgat aacgccgcgc ttgagaagct
     1021 gataacgcgt taccgccgcg acccagctat aaatggtgac aggcgtttcc cgttccccgg
     1081 tccaggcgat cactctccgc aggcgtttga gctgcttgtg gacgtcctgc gcggtttcta
     1141 ccggcgtgtc aaaaaattcg cggaaagtat cagtgatcaa ttccgggcgc gtattctgaa
     1201 taagataagt ttcgcaggat tgggtaatca gcgtcagcga tagcgtattg atcgctttta
     1261 aacgtcgatt ggcccgcgcc cagcgggaag attccatatt caggttgctg cgcatacctt
     1321 gtaacgccgt agtacgtcgc accagatcgc cccaggcttt atcgacaact tcaccatcgc
     1381 catgcttgat acagagttgc attaattgat attgcgcaac cagcaaactt tccagctctc
     1441 gatccacttc ttgtttgatc gatcgcggag aaaagagcaa atccgccata attgcacaca
     1501 caataccgat aacgatctcg ctacaacgtt cgacggcaaa ctgcggcgta agcaatggtt
     1561 ccggctgaat ggtgatcaca atgatcagcg cggtataacc ggccagcccc cacgcatacg
     1621 agttttctat tcgtaccagc gaggatatcc aggtacaaaa accggcccag atacagcaca
     1681 ccagaatcat caataatggt gctcggatca tcgcaatgat gattaccagt ccggcaatac
     1741 agccaataaa tgtgccgatg atgcgcaaaa agccacgata gcgaatagcg ccagaatacg
     1801 gttcacctcc cgcagcaaag gccggaccgg cggcaacaat cgccgctgtc agtactgccc
     1861 agcgtggcgt ttccagctgg aagtgaaagc caacaaacag cgccagtaca atggcggtcg
     1921 ccagttttac cgcaaagcga atatgttggt tagcaatgga gaaaataccc atcgtgatta
     1981 accaaactca cgcaggcgat gggccatttt acggaagaac gaatcctggc tttcgtcgcg
     2041 atcttgtttg ccagtgacca ccactgtagc agtggtgccc gcaggccaga tgttctcttg
     2101 ctggttgtcg agacgaatac gaaccggaac acgttgcgcc aaacgcaccc attcaaggtt
     2161 agagtcgatg gtcgccatcc ctttgtcgtc acgcgtgctg ctggcgttgg tgaccccagc
     2221 ggcaacacta tcaacagtcc ctttcagcac tttgttactg ccaagcggcg tgatctctgc
     2281 acgataccca gggcgcaccc cttccagctt agtttcttcc atataggcca gtacatagaa
     2341 ggagttctgt ttcaccagcg caaccgccgt tgatccacgg gtaataaact caccggtata
     2401 gacgttgagg ttggtcaccc agccatctgc tggcgcgcgg atcaccgtgc gttcaagatc
     2461 cagttttgcc agatcgcggg tcgcctgcgc tttcgctaac tgatgcagaa ctgtttgtag
     2521 tacgttgttg gcctggtcga tctcttcgcg agacatcgcc tgcacaccga gacggttacg
     2581 acgtcctgcc tcctggcgtt tctcctgtgc cagtacctga taataagcaa catcggcttg
     2641 cgcttcctca agcgcctttt gatagcgcgg ctggtcgatg gtgaacagta cctgtccttt
     2701 tttcaccagc tggttatcat gaacattcac ctgggtgata agtccagaaa cgtccggcgc
     2761 gatcgcaacg acgtcagcgc taaagcgcgc gtcacgcgtc cacggggatt cggtgtaata
     2821 gacccaggca ttaaaaattg cgatgaaggc cagaatgact aatacgaccg tgatggccgt
     2881 acgggagaat tttcttatta gtgttttcac ttcaacctca aacgaacagt cgcgatatca
     2941 aataaaacaa gcagcaatag agcgcggtgt tgaacaacgc cggatgccag acaaagtcgt
     3001 agatacctgt tggcacaagt acccggcgca ccagccagaa aatcgccagt gataaaagca
     3061 attcaaaaaa tatcggtggg aaggacagcc caaacaccac gataacggga aacagactca
     3121 tgttgacctt ggttgtatag agagagcagg cgttattatt ttcagcatct gtcgccgcag
     3181 agaagggcat ggaaagccgg gcgagagcaa cattgctgta gattgatatt taatatatta
     3241 gcgtaactgt tatgctgtta tctatattat gtgatctaaa tcacttttaa gtcagagtga
     3301 ataatggaac gactaaaacg catgtcggtg tttgccaaag tagttgaatt tggctctttt
     3361 accgccgccg ccagacagct acagatgagc gtttcgtcca tcagtcagac ggtgtcaaaa
     3421 ctggaagatg agttgcaggt aaagctgtta aaccgtagca cacgcagcat tggcctgacc
     3481 gaagccggta gaatttacta ccagggctgc cgtcggatgc tccatgaagt gcaggatgtt
     3541 catgagcaac tgtatgcctt caataacacc cccatcggga cgctgcgcat tggctgttct
     3601 tcaactatgg cacaaaatgt tctcgccggg ctgacagcaa aaatgctgaa agaataccca
     3661 ggtttgagcg tcaatctggt taccggaatt cctgcccccg acctgattgc cgatggtctg
     3721 gatgtggtga tccgcgtcgg cgcgttgcag gattccagcc tgttttcccg ccgtctgggc
     3781 gcgatgccga tggtggtgtg cgccgcgaaa agctatctca cacaatacgg cataccggaa
     3841 aaacccgccg atttgagtag tcattcatgg cttgaataca gcgtgcggcc cgacaatgaa
     3901 tttgaactga tcgcaccgga agggatctcg actcgcctga tcccacaagg aagatttgtg
     3961 actaatgatc cgatgacgct ggtgcgctgg ctgacggcgg gtgccgggat cgcctacgtg
     4021 ccgctgatgt gggtgatcaa cgagatcaat cgtggggagc tggagatcct gctgccgcgt
     4081 tatcagtcag atccacgccc ggtttatgcg ttatataccg aaaaagataa gctgccgctg
     4141 aaggtacagg tcgtgatcaa ctcgctgacg gattattttg ttgaggtcgg taaattgttt
     4201 caggagatgc acgggcgcgg gaaagagaag taattaatat tatactgtta cgttgtacaa
     4261 acctgtgcca acgggttccc ctcaccctaa ccccagaggg gcgaggggac cgttcgtgca
     4321 cgtagaagga ttaattatcc ttctgaaaat agtgaaatta ttacgcggta ccgccaacag
     4381 tcaggttatc aactttcagc gttggctggc ccacgccaac cggcaaactt tgcccttctt
     4441 taccgcagac acccacgccg ttatccagtt tcaggtcgtt gcccaccatc gaaatctgct
     4501 gcatggtttc gataccggaa ccaatcaacg tcgcgccttt caccggcttc gttactttac
     4561 cgttttcaat cagatacgct tctgaagtgg agaaaacgaa tttgccggat gtaatatcca
     4621 cctgaccgcc accaaagttc ggtgcataga taccgtactc aacagattca ataatttctt
     4681 gcggggtcga tttacccggc agcatatagg tgttggtcat acgcggcatc ggcagatgag
     4741 cgtaggattc acggcgaccg ttgccagtcg gcgtcatccc catcaaacgc gcgttgagtt
     4801 tatcctgcat gtagcctttc aggatgccat tctcaatcag cacgttgtac tggcctggcg
     4861 taccttcgtc atcaatcgcc accgaaccac ggcgatcgac catcgtgccg tcatcaacca
     4921 cggtacacag ttcagaagcc accagctccc cgacctgtcc actaaatact gaagtgccac
     4981 ggcggttgaa gtcgccttcc agaccgtgcc ccaccgcttc atgcaacagc acgcccggcc
     5041 aacctgcgcc aagtactacc ggcatggtgc ctgctggtgc ggcgacggca gaaagattga
     5101 ccagcgccat acgcactgct tcttttgccc atgcatccgc acggacttca ccgtcaagat
     5161 cggcaaggaa gaactcataa ccaaaacgac cgcctccgcc actggcaccg cgttcgcgtt
     5221 tgccatcttc ttcgaccaga acgctcacgg aaagacgcac cagcggacgg acatccgccg
     5281 ctagcgtgcc gtcggtggcc gcaaccagaa ttaattcata gacaccactg aggctggcag
     5341 tcacttcctg tacgcgcttg tccgcttcgc gggcaacctt atcgacgcga cgcaggatat
     5401 ccagcttctc ttcacggctc atgctttgca gcggatctac cgaggtatac aacgggctat
     5461 gctctaccgc gcccagcgtc tgtactttgc catcaccact atcacggacg atggtgcgcg
     5521 ccgcttgcgc actctgttcc agcgccagca ggctgatttg gtctgcgtaa gcaaatccgg
     5581 ttttttcacc gctgattgca cgcacaccaa cgccctgatc gatgttgtaa gaaccatctt
     5641 taataatgcg gtcttctaaa acccaggatt cgtgatagct cgactgaaaa tagagatcgc
     5701 cataatcaag gcgacgctcg gccagttgac cgaggatcgc gaacaagtcc tgatgtttca
     5761 ggccgttcgc cgctagcaat tgttcactta ccaggttaag actcatcgtt tttgctactc
     5821 gttagttact gcagtagagg attttttacg gctgccggat gcggcgtgaa cgccctgtcc
     5881 ggcctacggt tatgttctgg ctcgttggcc tttggcaacg attatcatat gagattgggg
     5941 caattacgcg ccctcgtcaa atcattgcgc tttttcttta cgcggttggc gcaacacttc
     6001 gttgatttgc ggatcgtcca gcggacccga aatgtgatag cgcaaaatgg agactttgct
     6061 ccacagcggc cccagcactt tactggcggc aaacactgcc gcgccaacaa tggggttaac
     6121 cgcaaaagcc gcagccacgc ccaccgtcgc agaaatctct ggtgcgacaa ccgcttccat
     6181 attcaggtcg cgacgcacca gatttaccga ccctttcatg gcaatgtccg cttccaggcc
     6241 atccaccagc gtgtcgtcgg tgtgcataac gccgtcttta atccacgcgg tgctgcgaat
     6301 ggagtcaaaa tagaaccctt caccaaaggt gtctctgaaa tcaaaacgca gcttacgcat
     6361 cagggcatca acgctcaata agcgcagcaa ttgcccggca tgtccggtat tgatttcggt
     6421 aatttcgcct ttgcccagtt gagtatgaat gatgccattc aacgtcgcct catctggttg
     6481 ccagggtgct ttgcgccagt gtaaatcgta atccacatta aatgacgact ggcgtatggg
     6541 cgtcgtgaca ccaaaaaatt ctgcggcggc atcaattttc tgcccacgca gttttccttt
     6601 cagcgaggtg cgttcatttc ccggattgtt aacccattca ccatcggcag tgagccgcga
     6661 aaaaccagta tcaatcagtc cattagtcag cgttaatgta ttgccagaaa tggtgatatc
     6721 actgtcaatg cgcccgaatt tttgccccca gaaccagcac tctgcgcatc gtatttgtgc
     6781 gtccggccag ccacggaagc taatgcgctc cgttgtcggg aaaggtgatg aattcgttga
     6841 gtcaccatga gttttcgcca cgcttgggtt gtaataaaga tatttgatat tcgccagcca
     6901 cggcgcgtta ttacgcatcg ccagcgtggc gttgatttca cgcccttgcg cctcaacctc
     6961 ggtgccattt gcagtcggtt gcgaaacaat actcaggtta ttccattgct gatttcccag
     7021 cgacaacata ggcgtacgta acgttatgtg ttgtgggaaa ctcgctgcac caccgacact
     7081 ctccgccgcg cctttctgga acagggccag ccactcggca ccattcatcg gcggcatatt
     7141 gagttcaacg ccactttgtt ccggcaacgg cgggagcgtt ttactgtctg ccgcccaaat
     7201 agcacggtcg agcgtcagct tttgaccaag caaccagcga ctattgaaat ggttatctgc
     7261 accagcctgt ccggttaact caaagctgtt gagattgcca tcaaccttaa cgtttaccgg
     7321 cagtggttca cccgcaggtt tggctaacgg tgaaggtaag tgactgctca cattcttcaa
     7381 atcgccgttt agctctacgt tataggtcgc gccagcatga taaggcagat caatgcccac
     7441 tttaccatcc cacgccacgc tgccacttaa tgcttcgttc accgcttcag gcaaaacgcc
     7501 ggttttcgct ggttgccagt taccgttgag attcaccgca acctggtagg cttttgcccc
     7561 ttctttggtg gaaaaatcca cgttcaacgg ctgattaaac cagcttgctg tcagtggttc
     7621 actttgcaga tcgccattga taaagctgaa tttaccgctc aaatttttca gggtgctgtc
     7681 gagtggtttg ataaacagac tgttattacg cagcgtcact tcacctttcg cggttaccag
     7741 ttcgccgttc agcgggatat caagatgtaa gcgagcattc acatcgccgt cgagctggag
     7801 ttcttgcagg gttgcaccca gagaatcttt cagcggtgtc tcatcaaagt aagggccgac
     7861 ggctttaccc ggacttttaa tgtcagcgtc aatcagcagt ttttctttcg agtagtcagg
     7921 gatcactgcg gtgagattac tcgcgcgcac gccgcccaga ttaacgccat cggttttcat
     7981 ccataaaccg tcgttaataa agtccagttc aatatcaagg ttagttaatg caggccagtc
     8041 cggctggaag gcaaacttcg cgttgcgcag cggcaccagc acttcaaact gaccttcatt
     8101 gtgtttatag gggaagagtt gcggattgcc accataaacc agcgtcgcat tatccgcttc
     8161 accgccctga atcgcgccac ttaagtaatc aaccaggtct ttacccatca agttttccgg
     8221 gaaatagcgc caggcttgtg aaccatcgtc ggtactgatg ccagccagaa tccccagcca
     8281 gggttcatcg ttagcaggtt gcaggtaacg aaaaccgccg cgcgcatgga cggctttggc
     8341 tttaacgtca atattacgtc catccagctg gaaacctttg tcattgttca gccagcttat
     8401 cgttgcctgg ccgtcggcga tttcaagtgg cgcacggaat accgtttcgt aaggcatctt
     8461 tgcctgcttc atcgatgcgg taagcaaacc attttctacg ctgccggaaa gcgtcccgga
     8521 gaagtgttcc gcacccggta ataatttcca ttgcttccag gccagatcgc tccacgatgc
     8581 ctgaaaacgg gtcttgtctg ccgcctgaag cgggatatcc agcgccagag tgttaatctt
     8641 gccgctcggt tgtgtggagc gccaaacatc acccagtgca ggtgaaagtt tcgcggccag
     8701 cgggcgtatg ccctccaggc ctgccagctc cagattactg gcgcgaatcc ggagttcgtc
     8761 actgcgttta ttgtctttgc cgccaacgtc ctgttccggt atccaggcca gcgtcaatgc
     8821 tccgctcggc cagggtttgc cgtccatcgt gatccgtgta tcgggaatag agaactgcca
     8881 tcccggattt tcacgcgtaa tatgcgcggt cagattatcc accgacagcg tatgcgtttc
     8941 cttctcgccc aaccagctgg caccgccctg tttcagccag acgtcgccac cggttacatc
     9001 gcctttatcg atcgtcatcc agccttcaag ggagaactgc gccgtttcca gcgcaatatt
     9061 gtcctgcatc catttaccga gccacggctt caggtcgatg tcatccgcct ggagccagac
     9121 gcgaccattg cttaacaacc cctcatcatc gcgcaaatcc atgcgcacct gcatcacgcc
     9181 gtgctgtccg gtaaggctgg agaggcttac caggccttcc gcacggtgtc gacgtggatc
     9241 gttcagccag gtgagttgtg ggatcgccag ctcggcgcgc tgaccggatg gcgtcaggaa
     9301 actgacttca ctgtcgcgga gatcaaaatg gtcgaactga cgaagaaaca gatcgctgat
     9361 gtgactggct tccaggctat cattaccacc gccgctggtg ataggagtgt tggtgcgaaa
     9421 gcgcagctgc cagaaagtga ggtcgcgaaa ctgccagcgc atatgtaaca ggctctgcca
     9481 gacatccagc gcaagagtaa cgcgtttaac cgaaaattcg ccgccatcct ttagttctgc
     9541 acggatgtcg tgtgcttcaa gcgtcgggcc aaaattctgc cagctggctg agagctgact
     9601 ggcttctacc ggcatgccag tcgcggattc tattttgttg aggatttccg gacgccaggc
     9661 atcaagatgc ggtaaagcaa tacgcaggcc gctaaccagc agggcagcga tcacaacgag
     9721 cgcggctcca gtaagcagta atatccccgg caatcgcctc acccgtcact ccttgtctgc
     9781 taaaaatgtg actcaaaaac cctttgccgg atggcggccc agcatctgtt tacatcatta
     9841 cgacgtcaaa ctgctcctgg ttatagagcg gttcaatttg tactttaacc tgtttgccaa
     9901 cgaaaatttc cacttccgcc agcgagtgtg actcttcgcc tttcaaggct tcagctactg
     9961 ccggagaagc atagaccagg aaacggtcgg aatcataagc atggtgtaca cgaacaatct
    10021 cgcgcatgat ttcatagcat accgtttcca cggttttcac cgttccgcga ccgtggcagg
    10081 ttgggcattc gttacatagt acgtgctcaa tgctttcgcg ggtgcgttta cgcgtcatct
    10141 ccaccagccc cagcgccgaa aaaccattaa cgctggtttt cacccggtct ttgctcaacg
    10201 cctgctccag cgagtgcagc acgcggcggc ggtgatcttc attattcatg tcgatgaaat
    10261 caataatgat aatcccgccc agattacgca accgtaactg gcgagcgata gcctgcgtcg
    10321 cttcaatatt ggtattgaaa atggtgtcgt ccagattgcg atgaccgaca aacgccccgg
    10381 tattgatgtc tacggtggtc atcgcttcgg tctggtcgat aatcagatag ccaccggatt
    10441 tcagttctac tttgcgttcc agcgctcgct gaatttcgtt ttcgacatca aagagatcga
    10501 aaatcggctg gcgtccggtg taatgctcca gcttgctggt catctcggga atgtactccg
    10561 aggtaaactc cagcaatgct tcgtaagtca ggcgtgagtc aacgcgaatg cggtcgagtt
    10621 cggcgtcggc gaaatcacgc agaacacgct gcgccagcgc cagttcgccg tacagctgat
    10681 aacgagtctg cgggcgcttt ttacgctcca tcactttggt ccagacgcgt ttcagataag
    10741 cggcatcgga ggccagttca gcctcgccaa ccccttcagc tgcggtacgg atgataaacc
    10801 cgccctgctc gtcgcaatac tctgcgacca cttttttcag gcgttcacgt tctgattcgc
    10861 tttcaatacg ttgggaaacc ccaacgtgag aagcccctgg cataaacacc agatagcgag
    10921 aaggcagcgt gatatcggta gtcaggcgcg cacctttagt gccaagcgga tctttcacca
    10981 cctgcaccat cagatcttgc ccctgacgga ccagttccga gatgtcgcgc accgtgaatt
    11041 gcttttgttc ttcacccgcc acacattcag tgtgcggcat gatatcggat gcatgaagaa
    11101 acgcggcttt atccagccca atatctacaa aagccgcctg catacccgga agtacacgac
    11161 ttacacgacc cttgtagata ttgcctacta ttccgcgtcg cgcctcacgt tcaatatgaa
    11221 tttcctgcag aataccgcca tcaatatacg ccactcgcgt ttccgaaggc gttacgttta
    11281 ccaacaattc agccgtcatg tttatccctt ttctcacgca gtgcgttaaa attacttaat
    11341 aattcatacg tttcaaccag cggtaagccg actacggcgt gatagctgcc atttatcttc
    11401 ctgacaaaac agccacccag cccctgaata ccgtatgcac ctgctttatc taacggttca
    11461 ccgctggcga catagcccgc gatgtcttcg tctgttaacg ttctgaaagt cacatcggtg
    11521 accaccaggc aatcgagaat gtgctggctg tcggccaacg ccactgccgt cattacctga
    11581 tgggtctgac ccgataattt gcgcaacatc tgcgccgcat gttctgcgtc gcgcggtttc
    11641 tccagcactt ctccattcag gataacgata gtatccgcac ccagcaccgg gagatcctgc
    11701 gccgtgtgcg tgacacccgc ctgtgctttc tcgcgcgcca gacgcacaac atattgctgc
    11761 gcgctctcct gcggctgacg ctgctcctca atgcccgtaa caatacgttc aaaggtcacg
    11821 ccaagttgcg caagtaactc ctgacgacgc ggagaaccgg aagctaaata cagagaagtc
    11881 atagaaacct ttattgcact gcaaactgct gacggacttt acgcatcagc aagaaaatcc
    11941 acggccagag cactccattg actacactac tccagaacac ttccggtctg aaagagacgt
    12001 taatcactaa aaactctgcc cagaaaacaa taatatccac caccagcgaa agcaacatga
    12061 cgaccagcgc ctgctgccat aatgcgaggt tgcggaaaag ctgatatttc agcgccacca
    12121 ggtaagcaat gatgctcatc gccaatacgc gtacgccaag tgtcgagccg ctgatcagat
    12181 ccagtatggc acccatcaca aaacctgtgc ccacatttac gcgatgaggc aaggccagga
    12241 tccaatacaa caagatgagt aacacccagt ttggccggaa aacaatcagg ttatccggcc
    12301 agggcatgat ttgcagcaac agcgcaatga ggaaagagag ccagattacc cagcgtccct
    12361 ggctacgata gctcgccact attgccctcc cggcgcacgc gcaggcggtt gagcaccact
    12421 ttgcggcggc gtagcccttt gtggagagcg attagcagcg ggctgtgtcg gcgcagcagg
    12481 cgcagtagct gcatttcctg tcgccggttg ctgcggagtc ggctgagcga tccccgttgc
    12541 cggttcgggt aactttggcc ccatcgcgtc tggcgaaggc aatacctgcg gcatcatctg
    12601 catcagacgt tcattagcaa cacgatgcac ctcttccggc gtcatcgggt tagcgccgtt
    12661 acgatccgcg ccccacagca gcagcagata acgcaaacgt tgcagtcccg cagtcggacg
    12721 cgcctgaatc acagtataag cgcgctgggt atcgagtttt acggaagaga caaccgcgac
    12781 cggatagcct tccgggaaac gaccgcccag accggaagtc accagcacat caccaacacg
    12841 aatatccgta ttcgctggca gatgctcaag ctgcaaatca tccgtacaac cgttaccggc
    12901 tgcaattacg cggatatcgt tgcgcagcac ctggattggc agcgcgtggg tcgcatcaca
    12961 aatcagcagc acgcgactgg tcagtttagc aacggccacc acctgaccaa caacaccttt
    13021 gtcactgatg accggctggc cttcataaac gccattaacg ctacctttat cgataacaac
    13081 ttgatcgcta taaggatcgt taaccgtgga gataacctga gtcaccattt tctgctcatc
    13141 ctgacgcagc ggggaaccca gcagctcgcg cagacgcgcg ttctcctgtt tgtattgtcc
    13201 aagcatcagc agttcactgt ttttcagcaa cagttcctga cgtaacgccc ggttttcaag
    13261 ttctagttgg tcacgcgagg ccagcgtctg cgatacgcca tccagcaatt cacgaggagc
    13321 attggaaaca aagtagaaag gactgacggc ggtatccata taagtacgga tttgactgaa
    13381 cgtccccagg cggctgtcgg caataataat gccgagcgcc accagcaccg ccagaataag
    13441 gcgaatctgt agcgacgggc cacggctaaa aattggcttc ataagttatg cgtattctcg
    13501 tatcagacca ggcagggtaa acagacgctt cccctgcctg catccgatta ctcttcgctg
    13561 aacaggtcgc cgccgtgcat gtcgatcatt tccagcgctt tgccgccacc gcgcgccaca
    13621 caggttagcg ggtcttcagc aacaacgact ggaatgccgg tttcttccat taacaaacgg
    13681 tcaaggttac gcagcagtgc gccaccaccg gtgagcacca tgccgcgctc ggagatgtcg
    13741 gaagccagtt ccggcgggca ctgttccagt gcaaccatta ccgcgctcac aataccagtc
    13801 agcggttcct gcagtgcttc gaggatttcg ttggagttca gggtaaaacc acgtggaaca
    13861 ccttctgcca ggttacggcc acgaacttcg atttcacgga cttcatcgcc cgggtaagcc
    13921 gaaccgattt cgtgcttgat acgttctgcg gtggcttcac cgatcagaga accgtaatta
    13981 cgacgcacat agttgatgat agcttcgtcg aaacggtcac caccaatgcg cacggaagag
    14041 gagtaaacca cgccgttcaa ggagataaca gcaacttcag tggtaccacc accgatatca
    14101 accaccatag aaccggtcgc ttcagaaacc ggcaggccag caccaattgc ggcagccatc
    14161 ggttcttcaa tcaggaagac ttcacgggca ccagcgccct gcgcggattc acgaattgcg
    14221 cggcgttcaa cctgggtcgc gccaaccggc acacaaacca gaacgcgcgg gcttggacgc
    14281 ataaagctgt tgctgtgcac ttgtttgatg aagtgctgga gcattttttc agtcacgaag
    14341 aagtcggcga taacgccgtc tttcattggg cgaatggcag caatattgcc cggcgtacgg
    14401 cccagcatct gcttcgcgtc atgacctact gcagctacgc ttttcggtga accggcacga
    14461 tcctgacgaa tggccaccac ggaaggctca ttcaatacga tgccttgtcc ttttacataa
    14521 atgagggtat tcgcagtacc caggtcaatg gacaagtcat tggaaaacat gccacgaaat
    14581 tttttcaaca tactaaggga taatcctgaa agctggggcg gaaaagaaaa tccgcttact
    14641 ttaccaacca cacgcagcag cgacaaggcg caaaaatcat ctgctacggt gaaaattagt
    14701 gcagttcgtt tcctttgtta caaatctctg cctgagtcca gaaaggctta atgcatcagc
    14761 agcattcctc gcctgtttgc aaccgcgtaa ggtcattcat ctgcatatgt gctgcaacaa
    14821 tctggcgagc agacaagcac actcccatga gacgcagcgc gcgttattct acgtgaaaac
    14881 ggattaaacg gcaggttaaa ccgagtatct ttgtgaatat tttttcacgt tagtatcaag
    14941 tggctgtgag gacgcgaaaa aatccccttg cccacctgta acaccgcgct gaatcagggt
    15001 ctgccactcg cttcgcgaac gcacaccggt ggcgtaaacc tgggtgctgg tcccggagca
    15061 ggcttccacc aggctttgaa ccagcagctg gttctccgtt cgcttctcaa tgtttctgac
    15121 cagccccgga tggagcttga gtaactcaac attaagttct ttgatccaac tggtgcttac
    15181 cagcgccaaa ccagcctggt tgacggctac ccgtacccct aaagcattca ctaaacgaat
    15241 aacaggttgt aaacgactga tatgttgacc tacatcggcc tctgcaagtt caataattat
    15301 gcgttttcgt tgcgatcttt cacattgcat taacgtatcg cgcagccaac gctgaaaacg
    15361 cgggcgaatc agcgactcaa cggtaacctg aatcgccaga ttttcctcag gccagtaacg
    15421 caatagtgga ataagacggc tgatttgcag gcggtcatac tcttccgata agccaaactg
    15481 caagaccatc ggcatatact ccgccgagct aacctcttca ttaccatcga agatgcggca
    15541 catgagttcg cgatgatgaa cctgaccttc gcgagtaacc gccggttttt gataaaggcg
    15601 cgggccgccg cgactcagca tttgctcgat aagcgtacgc cagcgaacat taccgcgtcc
    15661 tttttcaggc aacgagtcat cgtaaatagc ccagctattg ccgccctgca atcccgcatt
    15721 acgcgtggca gactctgcat gttccattac ctgctcggta tcctgaccac tacgccaggc
    15781 gcagatacca atgtggatca tatcgtcgcg atcgagcatt ttattgttcg gcaaggtatc
    15841 gacggctttg attaactgac cggcgatgct ctctgcctct tttaacgtcc ggtgcggtaa
    15901 cagcacagca aaatcactgc ggtggtaacg cgccagcagt gcgccagggt agcgcatcat
    15961 aaatgtcgat agcagattcg tcagagagaa gaactgttct tcaacctggc tgtgccccca
    16021 ggtatcactc aacatattga aatccggcag acgaatcatc atcacgatcc cgtgggtacc
    16081 tactttctct tgatcttcca gtaacgttgc taactgatta tcgaaaaaga gtcggttatt
    16141 gaggccggtt ttcatgtcct gggcggcata agagcggatc agcgtatcaa gacggctgtg
    16201 ttgttcgcgt gcgttctgaa tttcacgaag cagcgtatcc agcgcactgc tggttctggg
    16261 cggccattca tagatggttc ccaacacatt agagccacgc tcaccgttta agatacgagt
    16321 agcccgggtt tccagcaatt cttgcccggc aagttgccgt tgtaaccagc gtaccgccag
    16381 gaagagcata agaatgataa agccaatcgc ccccgtgagc ggcgcggtgg tcatcaacga
    16441 atggaaatag ttgcccatcg gatcctgata aaccagacgc agcgacatcc ccggatgctt
    16501 tatcaacgga acgctcagtt cgcgaaacag atcgctggtg ccaaccggac gataactacc
    16561 atttctggcg agggtataaa cctgtttatc accatgcagc aggtctacac gaacgatatc
    16621 tgctgacatc attaattcgg taatttgtgg ccttaatgcg ctgaagtcat tcgacacaag
    16681 atgggtatca atcgccgtcg ccaccgcctg aacgcgatga ctaaacttat actgaatggc
    16741 gttgtagaaa cttagcgaac agcccagcaa agtcacaaaa attgttaacc cggtgagcag
    16801 cgtaacaaag gccgaaaatt tcgtcgttaa tctcatcctt gtgttaactc cgatagtgag
    16861 gaagcgggca tactagcaaa tcagatttat ctcgcaattt attgcgcttc atcggctttg
    16921 cttttccatt agcgagtata gtcttcagaa attattttcc aatccatcat gcacatgagg
    16981 accacttatg caggcgttac ttttagaaca gcaggacggc aaaactctcg catcggtaca
    17041 gactctggac gaaagtcgcc tgccggaggg cgatgtcacg gttgatgttc actggtcgag
    17101 cctgaactac aaagatgcgc tggcaattac cggtaaggga aaaatcatcc gtaattttcc
    17161 gatgattcct gggatcgatt ttgccggaac tgtacgcacc agcgaagatc cgcgttttca
    17221 tgccggtcag gaggtgttac tcactggctg gggcgttggt gaaaaccact ggggggggct
    17281 ggcggaacag gcgcgagtga aaggtgactg gctggttgcc atgccgcaag ggctggacgc
    17341 gcgtaaagca atgattatcg gtactgccgg ttttaccgcc atgctgtgtg tgatggcgct
    17401 ggaagatgct ggtgttcgcc cgcaggatgg ggagattgtc gtgacgggtg ccagtggtgg
    17461 cgtcggcagt accgccgtgg cgctgctgca taagttgggt tatcaggtcg ttgccgtttc
    17521 tggtcgcgaa agtacccatg aatatctgaa aagtttagga gccagccgta ttctccctcg
    17581 cgatgaattt gccgaatccc gtcctctgga aaaacaagtc tgggctgggg caattgacac
    17641 cgttggcgac aaagtgcttg caaaagtgct ggcgcaaatg aattacggcg gctgcgtggc
    17701 ggcctgtggt ctggcgggtg gttttactct gccaaccacg gtcatgccat ttattctgcg
    17761 taatgtccgt ttacaggggg tggattcagt gatgacgcca ccagcacgcc gcgcacaagc
    17821 ctggcagcga ctggtcgccg atttaccgga atcattctat acccaggcgg cacaagagat
    17881 atctctggca gaggcaccga agtttgccga ggccatcatt aataaccaga tccagggtcg
    17941 cacgctggtg aaggttaact aaccatttag caaggattaa taagagaggg aactcatttt
    18001 gaaatcatta ttcaatcggt taacgggaaa agcggttagc cggacagctt tcgtcaaaca
    18061 tcttggtcat gaagttgtac aacatcatcc aaactggaaa gtcatgattt cgactgacca
    18121 taaattgatg cgcactgtta ctccactaaa cagccattat tgatacgcct ccgtcgcctg
    18181 ttaggtttat ggtgctttgc ctgggcgacg ctacgcttaa cctgttactg atttctggta
    18241 ccatggggtg aataatctga ttttatttgc ctgcaaatta accactcgaa cgtatttgat
    18301 gctgagcatt gtcagtcggt tgattttgcg tgctttagca ttcacatcta tccagacgat
    18361 gaagtgaaaa ttgcgtaatc ctcagcaacc gctgcgtaat gtcgtctatc ttgtcgcgat
    18421 cctggcatcc ctacattatt tgtggtctgt gaagattatc tcattgcagc ccctcatctt
    18481 cgcagggctg gctttacagc ttttcacctt acgttataag aagttccgtc gatgatggcg
    18541 ctaatttcgt gaattgtgcg gcttgttgca aattacacgg tgttgaaggt tatttacatg
    18601 ttagctgttg attatcttcc ctgataagac cagtatttag ctgccaattg ctacgaaatc
    18661 gttataatgt gcgacctcgt cctccctgac gcagtttttg cgctgcggaa aaggtgacat
    18721 tggcgcaacg aaggtatatt ttgttttttg ccggaggata gcagcagatc gctgcacaat
    18781 gtccgtcaag tctaacattg acactctggg gcaaaataga ccggcgtccc ggcctgctgg
    18841 aatttatcgc tatgcataca gctgtcgggg catacgcttt acagacggcg gtgaaacgcc
    18901 tgtcacaatc acactaaaca aagagtacgg aacccactca tggatattcg taagattaaa
    18961 aaactgatcg agctggttga agaatcaggc atctccgaac tggaaatttc tgaaggcgaa
    19021 gagtcagtac gcattagccg tgcagctcct gccgcaagtt tccctgtgat gcaacaagct
    19081 tacgctgcac caatgatgca gcagccagct caatctaacg cagccgctcc ggcgaccgtt
    19141 ccttccatgg aagcgccagc agcagcggaa atcagtggtc acatcgtacg ttccccgatg
    19201 gttggtactt tctaccgcac cccaagcccg gacgcgaaag cgttcatcga agtgggtcag
    19261 aaagtcaacg tgggcgatac cctgtgcatc gttgaagcca tgaaaatgat gaaccagatc
    19321 gaagcggaca aatccggtac cgtgaaagca attctggtcg aaagtggaca accggtagaa
    19381 tttgacgagc cgctggtcgt catcgagtaa cgaggcgaac atgctggata aaattgttat
    19441 tgccaaccgc ggcgagattg cattgcgtat tcttcgtgcc tgtaaagaac tgggcatcaa
    19501 gactgtcgct gtacactcca gcgcggatcg cgatctaaaa cacgtattac tggcagatga
    19561 aacggtctgt atcggccctg ctccgtcagt aaaaagttat ctgaacatcc cggcaatcat
    19621 cagcgccgct gaaatcaccg gcgcagtagc aatccatccg ggttacggct tcctctccga
    19681 gaacgccaac tttgccgagc aggttgaacg ctccggcttt atcttcatcg gcccgaaagc
    19741 agaaaccatt cgcctgatgg gcgacaaagt atccgcaatc gcggcgatga aaaaagcggg
    19801 cgttccttgc gtaccgggtt ctgacggccc gctgggcgac gatatggata aaaaccgcgc
    19861 cattgctaaa cgcattggtt atccggtgat tatcaaagcc tccggcggcg gcggcggtcg
    19921 cggtatgcgc gtagtgcgcg gcgacgctga actggcacaa tccatctcca tgacccgtgc
    19981 ggaagcgaaa gctgctttca gcaacgatat ggtttacatg gagaaatact tggaaaatcc
    20041 tcgccacgtc gagattcagg tactggctga cggtcagggc aacgctatct atctggcgga
    20101 acgtgactgc tccatgcagc gccgccacca gaaagtggtc gaagaagcac cagcaccggg
    20161 cattaccccg gaactgcgtc gctacatcgg cgaacgttgc gctaaagcgt gtgttgatat
    20221 cggctatcgc ggtgcaggta ctttcgagtt cctgttcgaa aacggcgagt tctatttcat
    20281 cgaaatgaac acccgtattc aggtagaaca tccggttacc gaaatgatca ccggcgttga
    20341 cctgatcaaa gaacagctgc gtatcgctgc cggtcaaccg ctgtcgatca agcaagaaga
    20401 agttcacgtt cgcggccatg cggtggaatg tcgtatcaac gccgaagatc cgaacacctt
    20461 cctgccaagt ccgggcaaaa tcacccgttt ccacgcacct ggcggttttg gcgtacgttg
    20521 ggagtctcat atctacgcgg gctacaccgt accgccgtac tatgactcaa tgatcggtaa
    20581 gctgatttgc tacggtgaaa accgtgacgt ggcgattgcc cgcatgaaga atgcgctgca
    20641 ggagctgatc atcgacggta tcaaaaccaa cgttgatctg cagatccgca tcatgaatga
    20701 cgagaacttc cagcatggtg gtactaatat ccactatctg gagaaaaaac tcggtcttca
    20761 ggaaaaataa gactgcaaaa gcgtcaaaag gccggatttt ccggcctttt ttattactgg
    20821 ggatcgacaa cccccataag gtacaatccc cgctttcttc acccatcagg gacaaaaaat
    20881 ggacactcgt tttgttcagg cccataaaga ggcgcgctgg gcgctggggc tgaccctttt
    20941 gtatctggcg gtatggttag tagccgctta cttacctggc gttgcccccg gttttaccgg
    21001 ctttccgcgc tggtttgaga tggcctgcat cctgacgccg ctgctgttta ttggactgtg
    21061 ctgggcgatg gtgaaattta tctatcgcga tatcccactg gaggatgacg atgcagcttg
    21121 aagtgattct accgctggtc gcctatctgg tggtggtgtt cggtatctct gtttatgcga
    21181 tgcgtaaacg gagcaccggc accttcctta atgagtattt cctcggtagc cgctctatgg
    21241 gcggtattgt gctggcgatg acgctcaccg cgacctatat cagtgccagt tcgtttatcg
    21301 gcgggcctgg agctgcttat aaatacggcc tgggttgggt attgctggcg atgattcagc
    21361 ttcctgcggt ctggctttcg ctcggtattc tcggcaagaa gttcgcgatt cttgcgcgcc
    21421 gctacaatgc ggtgacgctg aacgatatgc tatttgcccg ctaccagagt cgtctgctgg
    21481 tgtggctggc gagtttaagt ttgctggttg cgttcgttgg tgcgatgacc gtgcagttta
    21541 tcggcggtgc gcgtctgctg gaaaccgcgg cgggtattcc ttatgaaaca ggtttgctga
    21601 tttttggcat cagcattgcg ctctacaccg ccttcggcgg ctttcgcgcc agcgtgctga
    21661 acgacaccat gcaagggctg gtgatgctga ttggcaccat tgtgctgctt attggcgtgg
    21721 tccatgctgc tggcggctta agcaatgcgg tagagacgct gcaaaccatc gatccgcaac
    21781 tggttacgcc acaaggcgct gacgatattc tgtcgcctgc ctttatgacg tcgttctggg
    21841 tgctggtgtg ttttggcgtg attggcctgc cgcatactgc ggtgcgctgt atctcttata
    21901 aagacagcaa agccgtacat cgggggatca tcatcggtac gattgtcgtc gcaatcctga
    21961 tgttcggtat gcacctggcc ggagcgttag gtcgggcggt gatccccgat ctcaccgtac
    22021 cggacctggt gatcccgacg ttaatggtaa aagtgttgcc accgtttgct gccgggatct
    22081 tcctggctgc acctatggct gcgatcatgt cgacgatcaa tgcccagttg ctgcaaagtt
    22141 ccgctacgat cattaaagat ctctatttga atatccgtcc ggatcaaatg caaaacgaga
    22201 cgcgtctgaa gcggatgtcg gcggtaatta cgttagttct cggtgcgttg ctgctgcttg
    22261 ccgcctggaa gccgccagaa atgatcatct ggctgaattt gctggccttc ggtgggctgg
    22321 aagccgtttt cctgtggccg ctggtgctgg gtctttactg ggaacgcgcc aacgccaaag
    22381 gcgcgctaag tgcgatgatc gttggcggcg tactgtatgc cgtactcgcg acgctgaata
    22441 ttcagtacct gggcttccac cctatcgtgc cctcgttact actaagtttg ctggctttcc
    22501 tggtcggaaa ccgtttcggt acacccgtcc cgcaagctac cgttttgact actgataaat
    22561 aaagagtttt gccatgcctt ggatccaact gaaactgaac actaccggcg cgaacgcgga
    22621 agatcttagc gatgcgctga tggaagcggg tgccgtttct atcacttttc aggataccca
    22681 cgatacgcct gtatttgagc cgctgccggg cgaaacgcgc ctgtggggcg acaccgatgt
    22741 gattggtctg ttcgacgctg aaaccgatat gaacgacgtg gtggcgattc tggaaaacca
    22801 tccgctgctc ggcgcgggct tcgcgcataa aatcgaacaa ctggaagata aagactggga
    22861 gcgcgaatgg atggataatt tccacccgat gcgctttggt gaacgactgt ggatctgccc
    22921 aagctggcgt gatgtgccgg acgaaaacgc cgtcaacgtg atgttagacc cagggctggc
    22981 gtttggtacg ggtacccatc caactacctc tctgtgcctg caatggctcg acagcctcga
    23041 tttaaccggt aaaacagtca tcgactttgg ctgcggttcc ggcattctgg ctatcgcggc
    23101 gctgaaactg ggtgcagcaa aagccattgg tattgatatt gatccgcagg cgattcaggc
    23161 cagccgcgac aacgcggaac gtaatggcgt ctctgaccgt ctggaactct acttaccgaa
    23221 agatcagcca gaagaaatga aagccgacgt ggtggtcgct aacatccttg caggcccatt
    23281 acgtgaactg gcaccgttaa tcagcgtcct gccagtttca ggcggtttgc tgggcctttc
    23341 cggtattctg gcaagccagg cagagagcgt ttgcgaagct tatgccgaca gcttcgctct
    23401 ggacccggtc gtggaaaaag aagagtggtg ccgcattacc ggtcgtaaga attaaccttc
    23461 gcatcgccgt agggtgacgc agagcgaagt gcgagcgatg tcacaaaagg cacgttaatt
    23521 tgccgattat ttacgcaaat ttgcgtgcca aaattttcat tcataaagaa aaattgagaa
    23581 cttactcaaa tttctttgag tgtaaatttt agtcaacatt ttctaatatg atgattttta
    23641 tgagtaatta tcgcgccacg ctcattttaa atgcaattct ttgatccatc tcagaggatt
    23701 ggtcaaagtt tggcctttca tctcgtgcaa aaaatgcgta atatacgccg ccttgcagtc
    23761 acagtatggt catttcttaa ctcatgcgca tcggacaata tcagctcaga aatcgcctga
    23821 tcgcagcgcc catggctggc attacagaca gaccttttcg gacgttgtgc tacgagatgg
    23881 gagccggatt gacagtatcc gagatgatgt cttctaaccc tcaggtttgg gaaagcgaca
    23941 aatctcgttt acggatggtg cacattgatg aacccggtat tcgcaccgtg caaattgctg
    24001 gtagcgatcc gaaagaaatg gcagatgcag cacgtattaa cgtggaaagc ggtgcccaga
    24061 ttattgatat caatatgggt tgcccggcta aaaaagtgaa tcgcaagctc gcaggttcag
    24121 ccctcttgca gtacccggat gtcgttaaat cgatccttac cgaggtcgtc aatgcagtgg
    24181 acgttcctgt taccctgaaa attcgcactg gctgggcacc ggaacaccgt aactgcgaag
    24241 agattgccca actggctgaa gactgtggca ttcaggctct gaccattcat ggccgtacac
    24301 gcgcctgttt gttcaatgga gaagctgagt acgacagtat tcgggcagtt aagcagaaag
    24361 tttccattcc ggttatcgcg aatggcgaca ttactgaccc gcttaaagcc agagctgtgc
    24421 tcgactatac aggggcggat gccctgatga taggccgcgc agctcaggga agaccctgga
    24481 tctttcggga aatccagcat tatctggaca ctggggagtt gctgcccccg ctgcctttgg
    24541 cagaggttaa gcgcttgctt tgcgcgcacg ttcgggaact gcatgacttt tatggtccgg
    24601 caaaagggta ccgaattgca cgtaaacacg tttcctggta tctccaggaa cacgctccaa
    24661 atgaccagtt tcggcgcaca ttcaacgcca ttgaggatgc cagcgaacag ctggaggcgt
    24721 tggaggcata cttcgaaaat tttgcgtaaa cagaaataaa gagctgacag aactatgttc
    24781 gaacaacgcg taaattctga cgtactgacc gtttctaccg ttaactctca ggatcaggta
    24841 acccaaaaac ccctgcgtga ctcggttaaa caggcactga agaactattt tgctcaactg
    24901 aacggtcagg atgtgaatga cctctatgag ctggtactgg ctgaagtaga acagcccctg
    24961 ttggacatgg tgatgcaata cacccgtggt aaccagaccc gtgctgcgct gatgatgggc
    25021 atcaaccgtg gtacgctgcg taaaaaattg aaaaaatacg gcatgaacta attcaggtta
    25081 gctaaatgct tgattaaaaa ggcgctactc ggcatgggga agcgcctttt ttataggtgt
    25141 cacaaaggga gtgaccatga gaacaggatg tgaaccgacc cgatttggta atgaagcaaa
    25201 gaccatcatc cagggtgatg cattaaccga acttaaaaag atccccgccg aaagtgtcga
    25261 tctgatcttt gccgacccac cgtataacat cggtaaaaat tttgatggtc tgatcgaagc
    25321 ctggaaagaa gatctgttta tcgactggct gtttgaagtg attgcagagt gccatcgcgt
    25381 tctgaaaaag cagggcagca tgtacatcat gaatagcacg gaaaacatgc cctttatcga
    25441 tctccagtgc cgcaagcttt ttaccatcaa aagtcgcatc gtctggtcat atgacagttc
    25501 tggagtgcag gcgaaaaaac actacggctc catgtacgaa cccatcctga tgatggtgaa
    25561 agacgcaaag aactacacat tcaacggtga tgctattctg gtagaagcca aaaccggatc
    25621 gcagcgcgcg ttgatcgatt atcgcaaaaa ccctccacag ccttacaatc atcaaaaagt
    25681 accgggtaac gtctgggatt ttccgcgcgt gcgttatttg atggatgaat atgaaaacca
    25741 cccgacgcaa aaaccggaag ccttactgaa acgcattatt ctcgcctctt ccaacccagg
    25801 cgatatcgtt ctcgacccgt ttgctggtag ctttactacc ggtgccgtag ccgtcgccag
    25861 tgggcgaaaa ttcattggta ttgagatcaa cagcgagtac atcaaaatgg ggcttcgacg
    25921 gctggatgtc gcgtcgcatt actctgcgga agaactggcg aaagtgaaaa aaagaaagac
    25981 cggcaacctg tcaaaacgaa gcaggttaag cgaagttgac cccgatctca ttgcaaagta
    26041 aaggaagtgt aagcctggtt ttcagattat tcatttcgtg tatatttcct gccagacttg
    26101 gttaaacatg cacaggctca ggtaatgatt cgcaagtatt ggtggctcgt cgttttcgct
    26161 gtcttcgttt ttctgtttga tactttactg atgcagtgga ttgaactgct cgcaacagaa
    26221 acagacaaat gccgcaatat gaactcagtt aatccactaa aactggttaa ctgtgacgaa
    26281 ctgaattttc aggacagaat gtgaatttat atgacactta attcattcgt tttatgaatt
    26341 aatttcgtta tgttttcatc tggcatgaac attcttaata cgttatcgac cagtgcgggt
    26401 gcttgtttat aaagatcata acccgccata ttcattaacc agttttgaac aattccgctg
    26461 aaggcaccat caataataat catcacaaca tctaaatcga gattatttgc tatacaacct
    26521 tgttgctgac atccctgcaa tacttcgcgg agagtctgcg gattaaagcc catcttttcg
    26581 cgtatcactc cctcggccag catctcatca ttaaattcac atttgtgata taagattttc
    26641 agcaacgcct gctggcgggg aattttggca atatattgca agccgacaat caatttttca
    26701 cgcaattgtt gaaacgggtc atgctctaat ccagccgtca agtgttcctg gattaactcc
    26761 cgcaatgaag gctgttgcaa ccacatctca ttaaacagtt gggtcttgtt ctcgaagtgc
    26821 cagtagatag cgccacgcgt aacgttagcg gcgtcggcaa tgtcgttgag cgtcgtcttg
    26881 cttacgccac gctgcgcaaa ctgggcgatg gcagtttcaa tcagttcttg ccgggtcttc
    26941 agggcttcag ctttggttct ttttgccatg attaattatt caggaaataa atatattcga
    27001 cacaggatga gaaaatagcg aaggttaatc tatcacctaa tgtgtattta tacgagaggc
    27061 taatattgag ttgctataaa tcgtcaaata aataacactt attatttact taaaatacat
    27121 tcactctatc aatatatatt tcaatttacg aggatttgat tctacctctt tcaacccgcg
    27181 tcaaaataaa acagtagaac attaatcttt ttttgtgttt atgtgccttg agacgccagt
    27241 attcataact attccttaca tcgacgaatg ataatttgta ggatagcgaa ctgtattttt
    27301 ctttctgcga gttaacgcgt tgcctttttg ggtaaataac gcgcttttgg ttttttgagg
    27361 aatagtaatg acgaaacatg ccaggttttt cctcctgccc tcttttattc tgatctccgc
    27421 ggctttaatc gccggttgta acgataaggg agaagagaaa gctcacgtcg gtgaaccgca
    27481 ggttaccgtt catattgtga aaacggcccc gttagaagtt aagactgaat taccaggccg
    27541 caccaatgct taccgtatag ccgaagttcg cccacaggtt agcgggatcg tactgaatcg
    27601 caatttcact gaaggcagcg atgtgcaagc aggccagtcc ctgtaccaga tcgatcccgc
    27661 gacctatcag gcaagttatg acagcgcgaa aggcgaactg gcgaaaagtg aagccgccgc
    27721 cgccatcgcg catttgacgg taaaacgtta cgttccgctc gtgggtacga aatacatcag
    27781 tcagcaggag tacgaccagg ccattgctga tgctcgccag gccgatgccg ccgtgattgc
    27841 cgcaaaagcc acggtcgaaa gcgctcgcat caatctcgct tataccaaag tgactgcgcc
    27901 aattagcgga cgtatcggca aatcgaccgt gaccgaaggc gctcttgtca ctaatgggca
    27961 aacgactgaa ctggcgactg tccagcagct cgatcctatc tacgttgatg tgacccaatc
    28021 cagcaacgat tttatgaggc tgaagcaatc cgtagagcaa ggaaatttgc ataaggaaaa
    28081 cgccaccagc aacgtagagt tggtcatgga aaacggtcaa acctatcccc tgaaaggtac
    28141 gctgccattc tctgatgtga ccgttgatga aagcaccggc tccataaccc tacgtgctgt
    28201 cttccctaac ccgcaacata cgcttttgcc gggtatgttt gtgcgtgcac ggattgatga
    28261 aggcgtccaa cctgacgcca ttcttatccc gcaacaaggc gttagccgca caccgcgtgg
    28321 tgatgcaacc gtgctgattg ttaacgataa aagtcaggtg gaagcgcgcc ctgtcgttgc
    28381 cagtcaggcg attggcgata aatggttgat tagcgaaggt ctgaaatctg gcgatcaagt
    28441 cattgtcagc ggcctgcaaa aagcgcgtcc gggagagcag gttaaagcca ctaccgatac
    28501 ccccgcagat tttgcatcga agtaaggcaa tctgacatgg caaacttttt tattcgacga
    28561 ccgatatttg catgggtgct ggccattatt ctgatgatgg cgggcgcact ggcgatccta
    28621 caattgcccg tcgcccaata cccaaccatt gcaccgcctg cggtttctgt ttcagcaaac
    28681 tatccgggcg ctgatgcgca gaccgtgcag gatacggtga cgcaggttat cgaacagaat
    28741 atgaacggta tcgataacct gatgtatatg tcctccacca gcgattccgc cggtagcgtg
    28801 acaattaccc ttaccttcca gtccgggacc gatcctgata tcgcgcaagt gcaggtgcag
    28861 aacaaactcc agctcgccac gccgttgctg ccgcaggaag tacagcagca ggggatcagt
    28921 gttgaaaagt ccagtagcag ctatttgatg gtggcgggct ttgtctctga taacccagac
    28981 accacacagg acgatatctc ggactatgtg gcctctaacg ttaaagatac gcttagccgt
    29041 ctgaatggcg tcggtgacgt acagcttttc ggcgcacagt atgcgatgcg tatctggttg
    29101 gacgccgatc tgctaaacaa atataaactg acgcctgttg atgtgattaa ccagttgaag
    29161 gtacagaacg atcagatcgc tgccggacag ttgggcggaa cgccagcgtt accagggcaa
    29221 cagttgaacg cctcgattat tgctcagacg cggttaaaaa atccggaaga attcggcaaa
    29281 gtgaccttgc gcgtaaacag tgacggctcg gtggtacgcc tgaaagatgt cgcacgggtt
    29341 gaacttggcg gtgaaaacta taacgttatc gctcgcatca acggtaaacc ggcggcgggc
    29401 ctggggatta agctggcaac cggcgcgaat gctctcgata ccgcgaaagc cattaaggca
    29461 aaactggcgg aattacagcc attcttcccg cagggaatga aggttctcta cccttatgac
    29521 accacgccat tcgtccagct ttctattcac gaagtggtaa aaacgctgtt cgaagctatt
    29581 atgctggtgt tcctggtgat gtatctgttc ttgcagaata tgcgagcaac gctgatcccc
    29641 accattgcgg tacccgtggt gttgttaggg acgtttgcca tcctcgccgc ttttggttac
    29701 tccatcaaca cactaacgat gttcgggatg gtgcttgcca tcgggctgct cgtcgatgat
    29761 gcgatagtgg tggtggagaa cgtcgagcgc gtgatgatgg aggataagct cccgccaaaa
    29821 gatgcgacgg aaaaatcaat gtcgcaaatc cagggcgcac tggtgggtat cgcgatggtg
    29881 ctgtcagcgg tatttattcc aatggcattc ttcggcggtt ctactggggc aatttaccgc
    29941 cagttctcta tcaccatcgt ttcggcaatg gcgctttctg ttctggtggc attgattctt
    30001 accccagcgt tatgtgcaac actgctcaaa cccgtctctg ctgagcatca cgaaaataag
    30061 ggcggtttct tcggctggtt taataccacc ttcgatcata gcgttaacca ctacaccaac
    30121 agcgtcggca aaatcctcgg ttccacagga cgatatttac tgatctatgc actaattgtt
    30181 gctggaatgg tggtgttgtt tttacgtctt ccgtcttcct tcttacctga agaggatcag
    30241 ggtgtctttc tgactatgat tcagttaccc gctggcgcga cgcaagagcg gacgcaaaaa
    30301 gtgttggatc aagtgacgga ttactatctg aagaacgaga aagcgaacgt agaaagtgtc
    30361 tttacggtta acggctttag cttcagcggc caggcacaaa acgccggtat ggctttcgtc
    30421 agtctgaaac cgtgggaaga gcgtagtggt gacgaaaaca gtgcggaagc ggtaatccat
    30481 cgtgccaaaa tggaattggg caagatccgc gacggttttg tcattccatt caatatgcca
    30541 gccattgttg aactgggcac ggcaacgggt ttcgactttg agttaattga tcaggctggg
    30601 ctgggtcatg atgccctgac ccaggcccgt aaccagttgc ttggcatggc ggcgcaacat
    30661 cctgccagct tagtcagcgt gcgtcctaat ggtctggaag acaccgcgca gtttaaactg
    30721 gaagttgacc aggaaaaggc gcaggcatta ggtgtttcac tttctgacat caatcagacc
    30781 atttcaacgg cgttgggtgg gacttacgtt aacgacttca ttgaccgtgg tcgcgtaaaa
    30841 aaggtgtatg ttcaggcaga tgccaaattt cgtatgctgc cagaagatgt cgataaactt
    30901 tatgtccgta gcgccaatgg cgaaatggtg ccattctctg cctttaccac ttcacattgg
    30961 gtgtatggct ctccgcgact ggaacgctac aacggtctgc cgtcaatgga gattcagggg
    31021 gaagccgcgc caggaaccag ttccggcgat gccatggcgt tgatggaaaa ccttgcatca
    31081 caattaccag ctggcattgg ttatgactgg acgggtatgt catatcagga acgcttatcg
    31141 ggaaaccagg ctcccgctct ggtagcaatt tcctttgtgg ttgttttcct gtgccttgct
    31201 gcactctatg aaagctggtc aattcctgtc tcggttatgt tggtcgtgcc gttagggatt
    31261 gtcggcgtgc tactggcggc gacactcttt aatcaaaaaa atgacgtcta ctttatggtg
    31321 ggcttgctaa cgacaattgg cttgtcggct aaaaacgcta ttttgattgt tgagttcgct
    31381 aaagatctca tggagaaaga gggtaaaggt gttgttgaag cgacactaat ggcagtacgt
    31441 atgcgtctgc gtcctatcct gatgacctct ctcgccttta ttctcggcgt attaccgcta
    31501 gctatcagta acggtgccgg cagtggcgcg cagaacgcag tgggtatcgg ggtaatgggc
    31561 gggatggttt ctgcaacgtt gctggcaatc ttcttcgtgc cggtgttctt tgtggtgatc
    31621 cgccgttgct ttaaaggata aatcagaaac ataaaggcac tttcgggtgc ctttattatt
    31681 ttcagtgaaa cccataaaaa ttaataagat attcttcttc tcactcttta aaaactttct
    31741 atagttcccg ctcccttcac tatttttaca attcacataa tttgtcatta ttcattccgc
    31801 aagatttata acctccggtg ataaaatggc attcagctcg ttaataagag agttaactta
    31861 ttaagcgtta gcgtttatta ctgaggtaac accatgaaaa gattaattcc tgtggcattg
    31921 ctcaccgcat tgctggcggg ctgtgctcac gattcgccct gcgttccggt ttatgatgat
    31981 cagggtcgac tggttcacac caatacctgt atgaaaggca cgactcagga taactgggaa
    32041 acggctgggg ctattgctgg cggggcagca gctgttgctg gcctgacgat ggggatcatc
    32101 gctctgtcga aataactgca cagggcgcgg agtaccgcgc tctgtttaac gatacagtat
    32161 ctctccataa gataaaatat tgctatattc tgctgggtaa ttccctgctg atttagcatg
    32221 ttatatcgcg ttatttcgct gctttcgaaa taacgcatca atttaatttt cacacttcct
    32281 accagaaata tactcacgct aacaatcatt attctgctta gtaaaaaatg cgtaaatatt
    32341 ttgcgccaaa atgtggcgca tgtttcattt tcgcaccatt gcggggcgct gtttttatta
    32401 tttcctgtct acactcagct tattgcgacg cgaaatcgtg cgcaaaaact ggcactactt
    32461 ttgcttataa gaggatggcc acagacaggt aaaagacgtt tcccaaacgt cctataacga
    32521 taattttcgc ctcacaggaa gcattatgaa aaagatgatg atagccacac tggctgccgc
    32581 cagcgtgctg cttgccgttg caaatcaggc gcatgctggc gcgacgcttg atgccgttca
    32641 gaaaaaaggt tttgtgcaat gcgggatcag tgatggatta cctgggttct cttatgccga
    32701 tgctgacggt aagttttcag gtattgatgt agatgtttgt cgtggtgttg cggcagctgt
    32761 atttggtgac gacacgaaag tgaaatatac cccgctcact gcaaaagaac gcttcacggc
    32821 tttacagtca ggggaggtgg atttgctctc ccgtaatacg acctggactt catctcgcga
    32881 tgccgggatg ggaatggcat ttactggcgt cacttattac gacggcattg gcttcctgac
    32941 gcacgataaa gcggggctaa aaagcgcgaa agaactggat ggcgctaccg tctgtattca
    33001 ggcgggtact gataccgaac tcaatgttgc cgattacttt aaggcaaaca atatgaagta
    33061 cacaccagtg actttcgatc gctctgacga atcagcgaag gcactggaat ctggtcgctg
    33121 cgatacgctg gcctcggatc aatcacaact gtatgccctg cggatcaaat taagcaaccc
    33181 ggctgaatgg attgtcttac cggaggttat ctctaaagaa ccgcttgggc cggtagttcg
    33241 tcgtggcgat gatgaatggt tctcgattgt acgctggacg cttttcgcca tgctgaatgc
    33301 tgaagagatg ggcatcaatt cccagaacgt cgatgaaaaa gcggctaatc cagcaacgcc
    33361 tgatatggca catctgctgg gtaaagaagg cgattacggc aaggatctga agttggataa
    33421 taaatgggcc tataacatca tcaaacaggt gggtaactac tcggaaattt ttgagcgtaa
    33481 cgtaggttca gaaagcccgc tgaaaattaa acgtgggcaa aataatctct ggaataacgg
    33541 cggtattcag tacgcgccgc ccgtgcgtta agtcggtgtg aggtaacgag cgtcgctacg
    33601 gtggcgctct cttcagcgtt atgctttccg aggtttctat gtctcatcgc cgctcaaccg
    33661 ttaaaggctc actctccttt gccaacccta cggttcgtgc ttggttattc cagatccttg
    33721 ccgttgttgc tgttgttggc attgttggtt ggctatttca caacactgtg acgaatctca
    33781 gtaatcgtgg cattacttca ggttttgcct ttctggatcg cggcgctggc ttcggtattg
    33841 tccagcattt gatcgattac cagcagggtg atacctacgg acgcgttttc attgttggct
    33901 tactcaatac gctactggtt tctgcattgt gtattgtatt cgcttctgtt ctgggcttct
    33961 ttatcggtct ggcgcgactt tcggataact ggctgctacg aaagctttcc acaatttata
    34021 ttgagatctt ccgtaatatt ccgccgctgc tgcaaatctt cttctggtac tttgccgtgt
    34081 tgcgcaattt gcccggacca cgccaggctg tgagcgcgtt tgatctggcc tttttgagca
    34141 atcgtgggct ttatattccg tcaccgcagc tgggagacgg atttattgcg tttatcctgg
    34201 ctgttgttat ggctatagtc ctttctgttg ggctattccg ctttaataaa acataccaga
    34261 taaagaccgg acagctgcgc cgcacctggc cgatcgccgc agtgttgatc attggtttgc
    34321 ctttactggc gcaatggctt tttggcgcag cactacactg ggatgtccca gccctacgag
    34381 gctttaactt ccgcggcggg atggttttaa ttcctgaact ggcagcctta acgctggcgc
    34441 tttcggttta tacctctgca tttatcgccg agattatccg cgctgggatc caggcagtgc
    34501 cttatggtca acatgaagcg gcacggtcac tgggattacc caacccggtt acgctacgcc
    34561 aggtcattat tccccaggca ctgcgagtga ttattccgcc gttaaccagc cagtatctca
    34621 acatcgtcaa aaactcctct cttgccgccg cgattggcta tccggatatg gtttcgctgt
    34681 ttgctggcac cgtgctgaac cagacggggc aagccatcga gacgatagcc atgaccatgt
    34741 cggtctatct gattatcagc ctgactatct cgctgctgat gaatatctat aaccgccgca
    34801 tcgcgatcgt tgaacgctaa ggagtcatga tgacaaaagt attgctgtct catcccccgc
    34861 gcccggcgag ccataactca agccgcgcga tggtgtgggt gcgaaaaaat ctgttctcca
    34921 gctggagcaa tagcctgctg actattggct gcatatggtt gatgtgggaa ctgattccac
    34981 cgttgctgaa ctgggcattt ttgcaggcta actgggttgg ctcaacgcgt accgactgca
    35041 caaaagccgg tgcctgttgg gtcttcatcc acgaacgatt tggtcagttt atgtatgggc
    35101 tttacccaca cgaccaacgc tggcgaatta acctcgcatt actgattggg cttgtgtcga
    35161 tcgcaccaat gttctggaaa atactcccgc atcgcggtcg ctatattgcg gtatgggcgg
    35221 tgatttaccc actgattgtc tggtggctga tgtatggcgg gtttcttggt cttgagcggg
    35281 ttgaaacccg gcaatggggc gggctgacgc taactttaat tattgcatca gttgggattg
    35341 cgggggcgct gccgtggggg atcttactgg cgttaggtcg ccgctcccat atgccgattg
    35401 tgcgtatctt atcggtcatt tttatcgagt tctggcgcgg tgtaccgctg attaccgttc
    35461 tgtttatgtc ttcggtcatg ctgccgttgt ttatggcaga aggcaccagt atcgacaaat
    35521 tgatccgcgc gctggttggc gtgatcctgt ttcagtcagc atatgttgcg gaagtcgtgc
    35581 gaggcggatt acaggcgctg cctaaagggc aatatgaagc ggcagagtcg ctggcgttgg
    35641 gttactggaa aacccagggg ctggttattc tgccacaggc gttgaagctg gtgattcctg
    35701 ggctggtaaa taccatcatc gcacttttca aagataccag cctggtgatc atcatcgggt
    35761 tgttcgatct tttcagtagc gttcagcagg caaccgttga tcccgcctgg ttgggtatgt
    35821 cgacggaagg gtatgttttc gccgcgctga tctactggat cttctgtttc agcatgtcac
    35881 gttatagcca acatctggaa aaacgtttta acaccgggcg tacaccgcat tgaggacact
    35941 atgagccaaa ttttactgca acctgctaac gcgatgatta cgctggaaaa cgtcaataaa
    36001 tggtatggac aattccatgt tttgaaaaat ataaatttaa ccgtgcaacc gggagaacgg
    36061 atcgttctgt gtggcccttc tggttccggt aaatcgacaa ccattcgttg tattaatcat
    36121 ctggaagaac atcaacaggg acggatcgtg gtggatggca tcgaacttaa tgaagatatc
    36181 cgcaatattg agcgcgtcag gcaggaagtg ggaatggtct ttcagcattt caatctcttc
    36241 cctcatctga ccgttctaca gaactgtacc ctggcaccga tttgggtacg caagatgcct
    36301 aaaaaagagg ctgaagctct ggcgatgcat taccttgagc gggtgagaat tgccgaacat
    36361 gcccagaagt ttcccggaca gatttcgggt ggtcagcagc aacgcgttgc catagcacgt
    36421 tcactttgta tgaaaccaaa gatcatgcta tttgatgaac ctacgtcggc gctcgatccg
    36481 gagatggtga aagaagtgct ggatacgatg attgggctgg cgcagtcggg tatgacaatg
    36541 ttgtgtgtaa cacatgagat ggggtttgca cgaaccgtcg ctgacagggt gatttttatg
    36601 gatcgtgggg agatagtgga acaagctgca ccggatgagt tttttgcgca tcctaaatca
    36661 gagcgtacga gggcattttt atcgcaggtg atccattaat tgaatgttag ttcgaaaagc
    36721 aacaaggcca tcctttcgga tggcctttcg cttgatttga tgtctggcag tttatggcgg
    36781 gcgtcctgcc cgccaccctc cgggccgttg cttcgcaaag ttcaaatccg ctcccggcgg
//
LOCUS       DAPOZO010000048        36619 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPOZO010000048 DAPOZO010000000
VERSION     DAPOZO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.048"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            44..1093
                     /locus_tag="ROI21_002526"
     CDS             44..1093
                     /locus_tag="ROI21_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDX5111438.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1167..2024
                     /gene="nfo"
                     /locus_tag="ROI21_002527"
     CDS             1167..2024
                     /gene="nfo"
                     /locus_tag="ROI21_002527"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDX5111439.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2027..3115
                     /locus_tag="ROI21_002528"
     CDS             2027..3115
                     /locus_tag="ROI21_002528"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDX5111440.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIADRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3171..4421)
                     /locus_tag="ROI21_002529"
     CDS             complement(3171..4421)
                     /locus_tag="ROI21_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDX5111441.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4521..5462)
                     /gene="rihB"
                     /locus_tag="ROI21_002530"
     CDS             complement(4521..5462)
                     /gene="rihB"
                     /locus_tag="ROI21_002530"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDX5111442.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KKLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVDVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5592..6290
                     /gene="yeiL"
                     /locus_tag="ROI21_002531"
     CDS             5592..6290
                     /gene="yeiL"
                     /locus_tag="ROI21_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDX5111443.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6358..7608)
                     /locus_tag="ROI21_002532"
     CDS             complement(6358..7608)
                     /locus_tag="ROI21_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDX5111444.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICCGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7702..8640)
                     /gene="psuG"
                     /locus_tag="ROI21_002533"
     CDS             complement(7702..8640)
                     /gene="psuG"
                     /locus_tag="ROI21_002533"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDX5111445.1"
                     /translation="MSELKIPPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(8628..9569)
                     /locus_tag="ROI21_002534"
     CDS             complement(8628..9569)
                     /locus_tag="ROI21_002534"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDX5111446.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLMSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHGEFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAIVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(9993..11684)
                     /gene="fruA"
                     /locus_tag="ROI21_002535"
     CDS             complement(9993..11684)
                     /gene="fruA"
                     /locus_tag="ROI21_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDX5111447.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVSATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(11701..12639)
                     /gene="fruK"
                     /locus_tag="ROI21_002536"
     CDS             complement(11701..12639)
                     /gene="fruK"
                     /locus_tag="ROI21_002536"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDX5111448.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12639..13769)
                     /gene="fruB"
                     /locus_tag="ROI21_002537"
     CDS             complement(12639..13769)
                     /gene="fruB"
                     /locus_tag="ROI21_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDX5111449.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVNATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14137..15318
                     /gene="setB"
                     /locus_tag="ROI21_002538"
     CDS             14137..15318
                     /gene="setB"
                     /locus_tag="ROI21_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDX5111450.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15315..15569)
                     /locus_tag="ROI21_002539"
     CDS             complement(15315..15569)
                     /locus_tag="ROI21_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDX5111451.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15724..16296
                     /gene="yeiP"
                     /locus_tag="ROI21_002540"
     CDS             15724..16296
                     /gene="yeiP"
                     /locus_tag="ROI21_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDX5111452.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16519..17985
                     /locus_tag="ROI21_002541"
     CDS             16519..17985
                     /locus_tag="ROI21_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDX5111453.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGSDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGLAPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            18103..19089
                     /gene="yeiR"
                     /locus_tag="ROI21_002542"
     CDS             18103..19089
                     /gene="yeiR"
                     /locus_tag="ROI21_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDX5111454.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNERWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHHHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLASVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNIAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            19128..19841
                     /gene="lpxT"
                     /locus_tag="ROI21_002543"
     CDS             19128..19841
                     /gene="lpxT"
                     /locus_tag="ROI21_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDX5111455.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20253..20819
                     /gene="mepS"
                     /locus_tag="ROI21_002544"
     CDS             20253..20819
                     /gene="mepS"
                     /locus_tag="ROI21_002544"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDX5111456.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            21000..22556
                     /locus_tag="ROI21_002545"
     CDS             21000..22556
                     /locus_tag="ROI21_002545"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDX5111457.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQSLRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22638..24452
                     /locus_tag="ROI21_002546"
     CDS             22638..24452
                     /locus_tag="ROI21_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDX5111458.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPLARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKNHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            24453..25547
                     /locus_tag="ROI21_002547"
     CDS             24453..25547
                     /locus_tag="ROI21_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDX5111459.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25547..26572
                     /locus_tag="ROI21_002548"
     CDS             25547..26572
                     /locus_tag="ROI21_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5111460.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLEDKGWVLWAPIRFGA
                     TSINFSTDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26574..28163
                     /gene="yejF"
                     /locus_tag="ROI21_002549"
     CDS             26574..28163
                     /gene="yejF"
                     /locus_tag="ROI21_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDX5111461.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(28167..28511)
                     /locus_tag="ROI21_002550"
     CDS             complement(28167..28511)
                     /locus_tag="ROI21_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111462.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28844..30034)
                     /gene="bcr"
                     /locus_tag="ROI21_002551"
     CDS             complement(28844..30034)
                     /gene="bcr"
                     /locus_tag="ROI21_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDX5111463.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIEQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(30062..30757)
                     /gene="rsuA"
                     /locus_tag="ROI21_002552"
     CDS             complement(30062..30757)
                     /gene="rsuA"
                     /locus_tag="ROI21_002552"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDX5111464.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            30907..32667
                     /locus_tag="ROI21_002553"
     CDS             30907..32667
                     /locus_tag="ROI21_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDX5111465.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32792..33076
                     /gene="rplY"
                     /locus_tag="ROI21_002554"
     CDS             32792..33076
                     /gene="rplY"
                     /locus_tag="ROI21_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDX5111466.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(33215..34222)
                     /gene="yejK"
                     /locus_tag="ROI21_002555"
     CDS             complement(33215..34222)
                     /gene="yejK"
                     /locus_tag="ROI21_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDX5111467.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLKPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            34404..34631
                     /locus_tag="ROI21_002556"
     CDS             34404..34631
                     /locus_tag="ROI21_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDX5111468.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            34651..36411
                     /gene="pbgA"
                     /locus_tag="ROI21_002557"
     CDS             34651..36411
                     /gene="pbgA"
                     /locus_tag="ROI21_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDX5111469.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            36486..36562
                     /locus_tag="ROI21_002558"
     tRNA            36486..36562
                     /locus_tag="ROI21_002558"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36520..36522,aa:Pro,seq:ggg)
BASE COUNT         8939 a         9541 c         9057 g         9082 t
ORIGIN      
        1 aaccagttag tcgtaacctt atgcccggtt aaaggagagg gttatgacga atatcacctt
       61 acagaaacaa catcgtacac tgtggcattt tattccgggg ttagccctga gtgcagttat
      121 caccggggtc gccctgtggg gtggttccat tcccgcggtt gcgggtgccg ggtttagtgc
      181 cctcaccctc gcaatcttgt tggggatggt tttaggcaac accatctatc cgcacatctg
      241 gaaaagctgt gacggtggtg tgctgtttgc caagcaatat ttattacgtc tgggtatcat
      301 tctttatggc ttccgtctga cgttctcgca aattgccgat gtcggtatca gtgggatcat
      361 cattgacgtg ttgacgctgt ccagtacctt cctgctggct tgcttcctgg ggcagaaagt
      421 gtttggtctg gataagcaca ccagctggtt gatcggtgcc ggtagcagta tttgtggtgc
      481 tgccgcggta ctggcgactg agccggtagt gaaagcggaa gccagtaaag taaccgtggc
      541 tgttgcaacc gttgttatct tcgggaccgt cgcgattttc ctctacccgg cgatatatcc
      601 gctgatgtct caatggttta gtccggaaac tttcggtatc tacattggtt ctactgtgca
      661 cgaagtggcg caggtggtgg cggcaggtca tgccatcagc ccggatgcag aaaacgcagc
      721 agttatttcc aaaatgctgc gcgtgatgat gctggctccg ttcctcatcc tgctggcggc
      781 gcgtgttaaa cagctgtctg gggcgaacag cggcgagaaa agcaaaatca ctattccgtg
      841 gtttgccatc ttgttcatcg tagttgccat ctttaactcg ttccacctgt taccgcagag
      901 cgtggtgaac atgctggtaa cgctggatac cttcctgctg gcaatggcga tggcggcgct
      961 gggtctgacc actcacgtca gcgcgctgaa aaaagccggg gcgaaacctc tgctgatggc
     1021 actggtactg tttgcctggc tgattgttgg tggtggtgcg attaactatg tgattcaaag
     1081 cgtcatcgca taaaccacta catcttgctc ctgttaaccc gctatcatta ccgttttcct
     1141 ccagcgggtt taacaggagt cctcgcatga aatacattgg agcgcacgtt agtgctgctg
     1201 gcggtctggc aaatgccgca attcgcgccg ccgaaatcga cgcaaccgcg tttgccttgt
     1261 tcaccaaaaa ccaacgtcag tggcgtgccg caccgctcac gacgcaaacc atcgatgaat
     1321 tcaaagccgc ctgtgaaaaa tatcactaca catcggcgca aattcttccc cacgacagtt
     1381 atctgattaa cctcggacat ccggtcgctg aagctctgga aaaatcgcgc gatgcgttta
     1441 tagatgaaat gcagcgttgc gaacagctgg ggctttcttt gctcaacttc caccctggca
     1501 gccatctgat gcagatttca gaagaggatt gccttgcgcg tattgccgaa tccatcaaca
     1561 ttgcgctgga taaaactcaa ggtgtaaccg cggtgattga aaacaccgcc ggtcagggca
     1621 gtaacttagg gtttaaattc gaacatctcg cggcgattat cgacggcgtg gaagataaat
     1681 cccgcgtcgg cgtctgcatt gatacctgcc atgcttttgc tgccgggtat gatttgcgta
     1741 ctcctgccga atgcgagaaa acgttcgcgg attttgcccg tattgtcggc tttaagtatc
     1801 tgcgcgggat gcaccttaac gatgcgaaaa gcacctttgg cagccgcgtt gaccgccatc
     1861 atagcctcgg tgaaggcaat atcggtcatg atgcgttccg ctggatcatg caggacgacc
     1921 gtttcgacgg cattccgctg atccttgaaa ccatcaaccc ggatatctgg gcagaagaga
     1981 tcgcctggct gaaagcgcaa caaactgaaa aagcggtagc ctgaagatga acaaccggga
     2041 gaaggagatc cttgcaattt tacggcgtaa cccgctgatt cagcagaacg aaattgcgga
     2101 tatgctgcaa atcagccgtt cgcgcgttgc ggcgcatatt atggatttaa tgcgcaaagg
     2161 ccggattaaa ggcaaaggtt acattctcac cgagcaggaa tactgcgtag tggtggggac
     2221 aatcaatatg gatattcgcg ggatggcgga tatccgttac ccgcaagcgg cttctcatcc
     2281 cggtaccatt cattgctcgg caggcggcgt tggacgcaat atcgcccaca atctggcgct
     2341 gttaggccgc gacgtccatt tgctttcagt gattggcgat gacttttatg gcgaaatgct
     2401 cctggaagaa acgcgccgcg ccggcgtgaa tgtctccggc tgcgttcgtt tacatggcca
     2461 aagcacatcg acgtatctgg caattgccga tcgagacgat caaaccgtgc tggcgattaa
     2521 cgatacccat ctgctggatc agttgacacc gcaactactg aacgggtcgc gcgatttact
     2581 tcgtcatgcg ggcgtggtac tggctgattg caacctgaca gccgaggcgc tggagtgggt
     2641 ctttaccctc gctgatgaaa tcccggtgtt tgtcgatacc gtttcagaat tcaaagcggg
     2701 caaaatcaaa cactggctgg cgcatattca caccctgaaa cccactttac cggagctgga
     2761 aattttatgg ggacaggcga tcaccagcga tgctgaccgt aatgccgcag tgaatgcgtt
     2821 gcatcagcaa ggcgttcagc aactgtttgt ttatttgccc gatgagtcag tttattgcag
     2881 cgaaaaggat ggagagcaat ttttgctgac agcgccagcg catacgacag tagacagttt
     2941 tggtgctgac gatggtttta tggcgggcct ggtatatagc tttctggagg gaaacaattt
     3001 ccgtgacagc gcccgttttg cgatggcctg cgcggcaatt tcacgcgcca gcggcagctt
     3061 aaacaaccct accctgtctg ccgataacgc gctttcatta gtgccgatgg tgtaacaatg
     3121 ttgccggatt cgacgcttga ccgcgtctta tccggctcat aactcgcgca ctacgctaag
     3181 ccaataaaga atcctgcaat agtggcactc atcaggttag aaagtgtcgc cgccgccagc
     3241 gcgcgtaaac caagctgggc gatttccggt gcacggtgtg gcgcaaccgc agaaaacgcc
     3301 cccaccacca ccccgataga accaaagtta gcgaaaccgc acaacgcgaa ggaaataatc
     3361 gccacggttt tagcatccag agtgccagct gtttgcagat aaggtgagaa attgaggtaa
     3421 gcgacaaatt cattaatcgc cagtttctgt ccaatcaaac tcccggcaag attcgcatca
     3481 ctccagtcaa cccccatcac ccacgccagc ggggccagca ggtaacctaa aatggactcc
     3541 agcgaggcat gttcaaaacc aaaccagccg ccaacgccgc cgataatacc gttaatcaac
     3601 gcaattattg caacaaatgc catcaccact gtcgccacac ctgcggcgat tttcagtccg
     3661 gtcatagccc ctgtagcggc ggcttcaata atgctttttg gcggtgtttc ggtgaaagag
     3721 aggttattaa aggaaacctg cgaagattcg gtagccgggc ttaacaggcg ggcaaacaag
     3781 atcccgccgg ggatcgccat taacgatgcc gctagcagat attccacagg cacgcccagt
     3841 gcggcgtaac caatcattgt cgaaccagca atcgaggcca tgccactaca aatagccgta
     3901 aataattcat tgcgattcag acgatcgata aagggtttga cgattgccgg aatttcgttt
     3961 tgcccgagga aaatggtagt gaccgcgacg aatgactcga tcttgctgat atttaatgct
     4021 ttctggaaga taccgccgag aatgcgaatt aagatcccca tcacaccgat gtagtagaga
     4081 atactcacca gtgctgtaac gaagataatt gccggtaata ccctgaaacc aaagataaat
     4141 cctgcgccat caaataaggt atccattttc ggtccgacca gagaaccaaa gataaatgcg
     4201 ctacctgcat cgctgtacgc catcacttta tgcacgccaa aagcgacttt ttctgcgacc
     4261 caacgccctg gtggtaacca aagcataatg ccgccaatca cgacctgtaa cactaacgcc
     4321 gcgccaacgg tacgcaggct gatcttcttc ttgtttactg acagcaaaaa cgcaattgcc
     4381 agcaatacca ccattcccag aacacttctc atgacatcca tagttattat ctcttcatgt
     4441 cattttgggc gacggctttc tgcccatcag aaatagccgg gcctgcatct tgcgatactg
     4501 tcccggcttg ttatcaacac ttaatgggtt ttgatgtagc cgcgcacgca ctcttcgact
     4561 aatccccaga accagtcggt atcaatagtg atgccgactt tggtattggc gggcttgcca
     4621 agaacgccca gctcgtcgca gacggtacgc ccatagcaag ggccactgtt aacgtccacg
     4681 tcgacgtaca tctcctgggt tttaatgcca tcagggttaa tcagataacc gatgcaggtg
     4741 gcgtcatgca ccgggccgcc agcaaggccg tagttttcga actgcgtttt gagagtgaag
     4801 ttcatgatgt cgctgaacag ctctccggcg gggccgcctg ccctttccat ccgtgcaatc
     4861 acgtccgggg tgcaaacggt ctggttggtg agatcgaggc ccatcatcac taatggaacg
     4921 ccggaggtga acaccacgcg tgcggcttcc gggtcagcaa agatgttgaa ctcggcggat
     4981 ggcgtaaagt tgccagtacc gtaagcgccg cccatcagca caatttcacg gattttcggc
     5041 aggatcgcgg gttgcatacg cattgccacc gcgatgtttg aaagtggacc aaccggcacc
     5101 agagtgatat cgccatcgct tgccatcagg gtatcaatga tgtatttcac cgcatgggta
     5161 ctttctgcct ggcgggtcag cggctcgaat accgggccat ccagtcctgt ttcaccatga
     5221 atattgtcgg caacgatttg ttgacgcata atgggctgtg gcatccccgc ataaaccgga
     5281 acattaatct ccagtttctg gcaaacattc aggccattaa ttaatttttt atcaagcgtc
     5341 tgattacccg ccacaatggt gatgcctaat aaatctattg ccggatgttt cgctgccatc
     5401 attatggcaa tagcatcatc atgacccgga tcacaatcca gaataatttt tctcttttcc
     5461 attgtttatt tcctctgttt ccagttgcgt tattttttct acagcaaaga aaattcgacc
     5521 gggccgatga ttgaatctta acaacagcgt aagtatgcta aatatgagaa atctcatagc
     5581 ggataaacat cgtgaaagaa atccacaata atgaccttaa gcagcaattg atgagtgaat
     5641 ccgcgtttaa ggattgcttt tcaacggatg tttcagccga tacgcggctg tttcattttt
     5701 tagcgcgtga ctacattgtg caggaagggc aacagccgtc ctggctgttt tacctgacgc
     5761 gaggccgcgc caggctttac gccacgctag ctaatggtcg cgtgtcgctg atcgatttct
     5821 ttgccgctcc ctgttttatt ggcgagattg agttaatcga caaagaccat gaaccgcgtg
     5881 cggtacaggc tattgaagag tgttggtgcc ttgcgctccc tatgaaacat taccgtccgc
     5941 tgttattaaa cgacacgcta tttttacgaa aactctgcgt tactttaagc cataaaaatt
     6001 atcgcaatat tgtttcttta actcagaatc aatcatttcc gttagtgaat cgcctggcag
     6061 cttttatatt actctcgcag gaaggtgatc tttatcacga aaagcatacg caagcggcag
     6121 agtatttagg cgtttcttat cgacatcttt tatatgttct agcgcaattc attcacgacg
     6181 gtttattaat taaaagcaag aaagggtatc taattaaaaa cagaaagcag ttgtcaggac
     6241 tggcactgga gatggacccg gaaaataaat tctcagggat gatgcagtaa aaattatttg
     6301 caatagagcg attgccggat gtgacgctaa ataagcgcgt acatccggca atgaaactca
     6361 agctaaacca ataaagaacc cggcaatggt cgcgctcatc aggttggaaa gcgttgctgc
     6421 tgccagcgcc cgtaaaccaa gctgggcgat ttccggtgcg cgtttaggcg aaatagccga
     6481 aaatgcgcca acgacaacac cgatagaacc aaagttagca aaaccacaaa gcgcaaagga
     6541 gataatcgca atggttttca cttccagcgt gccgcccgtt tgcaggtatg gggagaaact
     6601 caggtaagcg acgaattcgt taatcgccag tttctgccca atcaggctac ccgcaagatt
     6661 ggcatcactc cagtccacac ccatgatcca cgccagcggt gccagcacat agccaaaaat
     6721 actttccaga gaggcattgg cgaaaccaaa ccagccgcca attccgccga taatgccgtt
     6781 gatcagcgca ataattgcga caaacgccat taccaccgtc gctacaccag cggcgatttt
     6841 caacccggtc atcgcaccgc tcgccgctgc ttcgataaag ctttttggcg gcgtttcgct
     6901 gaacgacaga ttttcaaatg tgacctgcga aggctcggtt gccggactaa gaatacgtgc
     6961 aaacaaaata ccaccaggga tcgccatcag cgatgccgct aacaggtagt caattggtac
     7021 gcccattccg gcataaccaa tcatcatcga acccgcaatg gacgccatcc cgcaacaaat
     7081 tgcggtaaac agctcgttgc gattcatgcg atcgataaac ggtttaacga tcgccgggat
     7141 ctcattttgc ccgaggaaaa tagtggtaac cgcaacaaaa gattcgattt tgctgatgtt
     7201 gagagctttc tgaaaaatgc tgccaagaat gcggattagc agccccatca cgccaatgta
     7261 gtacagcagg ctgatgagcg cagtgacgaa gataatcgcc gggagtacgc gaaaggcgaa
     7321 gataaaaccc gcaccgtcaa agaggacatc cattttcggc ccgaccagcg aaccaaaaat
     7381 gaaggcgcta ccggcatcac tgtaagacat cactttatgt acgcctaatg ccgcctgctc
     7441 taccgcccat tttcctggtg gaaaatagag catgatgccg ccaatggcga tttgtagcag
     7501 caacgcggct ccaaccgtgc gcaaactgat gctcttttta ttcactgaca acaaaaatgc
     7561 tattgccagt aacaccacca tccccacaac acttctcatt atatccataa tgattttccc
     7621 ttcatgccag taaacccggc gtcagcgcca ggttttggta tgtatgatca gtacgggcga
     7681 cggctttctg cccgtcagaa attaacccgc gagacgctgg tattctttgg ctatttcact
     7741 cgccaggatg gcgttgttga acaccagctg gatgttggat ttcaggctgt caccgccggt
     7801 caactcagca acgcgcgcca gcaggaatgg cgtactttct ttaccaataa caccctgcgc
     7861 ttcagcttca gctaccgcct gatcgatcgc cgcattgata gtgtgttccg gcatagcaaa
     7921 ctgttccggg atcgggttcg ctaccacgag gccaccgttc agcccgcttt gccatttcac
     7981 cgccattgca cgggcaattt cgctggcgct gtcgagacga atgctcacgt caaacgggct
     8041 ggtgcggcag aaaaacgcag gcagcgcttt ggtctgatag ccaattagcg gtacaccgaa
     8101 agtttctaaa tactcagtgg ttaatcctaa atcgagaata gatttcgccc cggcacaaac
     8161 aacggtgaca ttagtatttg ccagctcttg caaatcggca gaaatatcga aggtatgttc
     8221 cgccccgcga tgcacaccac caattccccc ggtggcaaat actttaattc cggcaagcgc
     8281 cgcaataatc atcgttgaag caacggtagt tgcgccattt tttccggcgg caacaacaaa
     8341 aggtaaatcg cgacgactaa ctttggtcac gttatgccct tcacgaccca gcaattcaat
     8401 ttcttctttg cttaaaccca ctttcatcac gccgccaata atggcgatag tggcaggtac
     8461 agcgccctgt ttacgaatag tttcttcaac ttcaattgcg gtctgggcat tttgtgggaa
     8521 cggcatcccg tgagaaataa tggtcgattc cagcgccaca accggttttt tgttttttaa
     8581 agcgtcctgc acttccgggg aaatttgtaa taattcaggg ggaattttta attcagacat
     8641 tcggcgttct ccactaacga tataacgttg gcaatcgata aatcggggtt attggtgtat
     8701 tcacaggaga gcgccattga cgaacatccc tgtgcgaaac gaacagattc ggcaaacggc
     8761 attccgtcta cccaacacga agcaagtccc gccatcatgg catcgcccgc tccggtaaca
     8821 ttaatgacat tggttttgat cggcgcagac caaccacttt caccgctgat atcactgtaa
     8881 taaacgccgt cgccgcccat gctcaatacc agtcggttca ggccatgttg atggaaccag
     8941 gcagcaacta ttgccacatc ttcacgccct gacaaagcaa tcccactcag ggtttccgct
     9001 tcaaggcggt ttggcttgag agtgtggatt tgatttagac ggtcgcgcac tttgacacat
     9061 ttccatgcgg aaaccggatc gacaaataca ggtacattgg cggcattatc cagaatccat
     9121 gccagtgcct cttcactgat attacagtct gcgacaatga cctttgccct ctgaataaat
     9181 tcaccgtgct gtgcgagata ttcagctgta atagcgttgc taatattcat gtcatttata
     9241 gcaaccagca tttcaccggt attatcgagt aatgataaat agcttgacgt attttctccc
     9301 ggcacaatca ggcatttatc gacataaacg ccagattgat tggtttgcgt tagcagcgat
     9361 tgaccataaa agtcactgcc tactgcgctc attagccagg ctttgttccc cagcaacgcc
     9421 agattttgtg caatattgcg cccgactcca ccaggcgtaa attttatttt acctggattt
     9481 gaatccgcat aatttaatga ttcatgtgaa tatccggcga catcaatatt cgccgaacct
     9541 ataattacga cataatcctt ttcgcgcata gcacgtcctc tggtaaaaaa ttaccaaccc
     9601 tgtcaatcca caagaatatt gatactgcaa agacggttta aacatgtgtt catatttaaa
     9661 catgtgctca tagtatgcat gttttacgta aagtaaatta ccgccagaga ggcaaaatgg
     9721 gcagatttga gcaggctcac aaaacgcgat taaatgcctg aattacaaaa acggggaatt
     9781 tgtgacggga tgcacagaac aggaacaata aaaaagggca gaaattaatc tgcccttgag
     9841 gaaataacct tagtgtttga tgaacgtttc aggtttctac tgaaagataa aaccatttaa
     9901 attcataaaa ttacattcac aaaccggtca ttccctttgc gctcatcaaa tgttccaggg
     9961 caggaaattt ctgccctgga acacaccttt tattacgctg ctttcgctac tgcgtccact
    10021 tccggacgtt tcaggaaggc ataggccaaa cccgccacca gcgtaccggc aataatggct
    10081 accaggtaac ccaataccgg cgtaatcgcg ccagggatca gcagaacaaa cagaccaccg
    10141 tgcggtgcca tcagtttcgc accaatcgcc attgagattg cgccagtcag cgccccaccc
    10201 acgatacagc acggcagcac acgcatcgga tcacgagcag caaacggaat tgcaccttcc
    10261 gaaatgaagc acagtcccag taccagagcg gctttgcccc cttcctgctg cgctttgtcg
    10321 aatttgcgac gcgccaccat tgttgccaga cccattgcca gcggtggcac catacctgcc
    10381 gccataatcg ccgccatcgg gccataggtt tgagtactca gcagacccac accgaatgcg
    10441 tacgctgctt tgtttaccgg accgcccatg tcagtacaca tcatgccacc gaggatcgcc
    10501 cccagcagaa ccgcattcgc agtccccatg gtctgcagcc agtgagtcaa cccttcgaga
    10561 atgccagcaa ccggtttacc gatcaggtaa atcatcgcca gaccgaccac cagactggaa
    10621 attagcggaa tgatcaggat cggtttcagc gcctccatac tctgcggcag tttcaattgc
    10681 gtactgatta acttcgcaat gtaaccagcc aggaagcccg caataatacc gccaatgaag
    10741 ccagaaccgg tgctgaccgc cagcataccg ccaatcagac ccggcgtgag acccggacga
    10801 tcggcaatgg aaaaggcaat ataacctgcc agtaccggca ccatcagcgc aaaggctgaa
    10861 ccaccaccaa tctgcatcag cgccgcagcc aacgtacccg gctctttaaa cgcttcgata
    10921 ccaaaagcaa aagaaagcgc gatacacaga ccacctgcaa cgaccatcgg cagcatgtaa
    10981 gagacgcccg tcagcaagtg acggtaagcg cctgcactct ctttcttacc ttcagtggtc
    11041 gctgtttgag ttttgcccgc cggttcatac ggcgttgctt cagcaaccgc tttatccagt
    11101 tcctgcgcgg ttttcttcag cgccagaccg gtagaggtac gatacatcgg tttaccagca
    11161 aatttcgcca gatccacttc aatatctgcc gccacaatca ccagatccgc tgcggctact
    11221 tcttcagggg tgattgcatt acccgcgcca acagaaccac gggtttcaac tttcacccac
    11281 cagccacgtt ttttcgcttc ggtttcaatg gcttcagccg ccataaaggt gtgtgctacg
    11341 ccagtcgggc aagcagtcac cgcaactacg cgtttcggac cgctggcggc aaccggtgct
    11401 gtcgcagaga ccggcgcagt gtaaggtttc gcatggcctt tggcttcact caggaacagc
    11461 tcagggtgcg caactgcccg ggaaatatcg cccagccaga catttttacc gttcagcgcg
    11521 ctgtcgttcg ggatggaatc accgagaaca atcgccattt cagcgtcgtt cggattgtcg
    11581 atgatttcca gttttgcttt tcgcgccgcc gcgcccagca gggtcttcgc catataggcg
    11641 cgtgcctgac cgagattagc gtcaataatc agcagcgttt tcattatgcc tctcctgctg
    11701 tcagttaaaa ggttgtaagt cgacgcgcgc catcattgcg gccaactgcg gacgatcggt
    11761 aatacccaca ttgctttgac ttaccgccag ggctgcaaca gctgtcgcca gacgcagtgt
    11821 gtgttcactg gattcacgca tcagcaagcc ataaatcagg ccaccaacca tagaatcccc
    11881 tgcgccaacg gtgcttacga catcgactga cggtggtttg gcgatccatt cgccggaggc
    11941 attaacccaa agcgcgcctt cggcacccag tgaaataaca acatgcgcaa taccttgttc
    12001 acgcagcgca tgtgcagctt caatcacatc tttcatttca ggcagtttac ggcctgccca
    12061 gatttccagc tcgcggcggt taggtttcac cagccacggt gccgctttca aacctgctac
    12121 taacgcttca cggctactat caaagataat gcaaggacac tgactacgca ggcgagtcat
    12181 ccagtcggtg aacgcttccg ggctgacgcc tgacggtaag cttccgctga cacagaccat
    12241 atcgaactga ccgagccagc tcagagaatc agtcacaaag cgttcccagt cggcgggggt
    12301 gacttcaaaa cccgagaagt tgaagtcggt cacttcgccg tctttttccg tcagcttaac
    12361 gttaattcgg gtgcgcccct gtacaacctg gaaacggttg gcaatgccca gctcgctgaa
    12421 cagttgctga aaaccatcct gattgtcttt accgaggaag ccgccaacgg tgacatcaat
    12481 tcccaggtct tttaatactt tggccacgtt gatgccttta cccgccgcat gcagaccggt
    12541 ggttttcacc aggttcactt cgccgcgttc aatttccggg cagaaaccaa caaggtcata
    12601 agccggatta agggtgatag tagcaacacg tctgctcatt atgcgccctc cccaagacca
    12661 gcagcgatag cgtcgccaat ggctttcagc gcctgttcag catctgcacc ctgggcggta
    12721 aagcgtaggc gatgaccttt cttaacgcca agtgccacaa ctttcatcag actacgtccg
    12781 tttgccggtt tgccggtgcc atcaaggttt gtcacggtaa tatcactgtt aaattgttta
    12841 atggtattga ccagcatggt acctggacga gcatgcaggc cgtgttcatt gcgcaccaca
    12901 aactccgcgc ttaacacatc gtcggtcggc gcatcatcgc tggtcagcag cgccagcaac
    12961 gttgccgcat ccgctttcag caagcggtca gctttattat cgagcaacaa atcggcgaga
    13021 cgcttaagaa ccgcaatggg ctgatcgtca ttcatcgcca cactcaccag catggctgcc
    13081 gtttcgccgt ccacatcaaa agcatttgcc gcacggctta ccgcaatcgc gctacgcaga
    13141 ttgccttcgg cgctatcgct cagccagata ccctgtccga ggttcagcgg ttgttcattg
    13201 atggctttgg tgacgaaagt ggcgttaact gcccccgctt ctttcagacg tgcagcgttc
    13261 agcgcctgaa gagtcagcag atcgctggcg acgatatcca gcgtcagcat ttcgttgtcg
    13321 agcttcagct gctcactctg cttttcgccc atcagcaatg cgcgaagttc ttctgctgtt
    13381 gttgctgact tcagttgttc agcaacggaa tcatcgctca gtacgtgggt cagctggcgt
    13441 agcaggccca gatgttcatc cgagctggca gcaataccga tcgccacgta cgctacctgg
    13501 ccgtcacccc aggtgacgcc ttccgggaac tgaaatacct gaacgccggt tttcagcacc
    13561 tgatcgcggg tgtcggtagt gccgtgtgga atagcaatac cattgccgag gaacgtagag
    13621 gtttgctgtt cgcgcgccag catgccattg acgtagcctt cagctacatt accggcctgc
    13681 accagcgccg cagcgacctg gcgaatcgcc tcttctttgt ctccggcctt ttcgcccgga
    13741 tggatgtcct gtacggataa ctggaacata gttctcctct cttgctgaat tgaaacgatt
    13801 cagcttctat gagaaaaaaa gcgccaacct ggcttagggt taaagacaag atcgcgctga
    13861 aacgtttcaa gaaagcataa tacttctgtt tcagcacgca aggaaagccg taaagttatg
    13921 agcaaaagtt tgatgtgctg cacaattttt tgccattttt cctaattgct gatgggaaaa
    13981 tccgttatca gcaatttcat ttcagcagcg tcagcagcca atcagcaaca gtgaaatggc
    14041 attttgatta ttcgtggcgc ttttgatttc gctgtctgtt tattttctga caagcagcgt
    14101 aaactccgcg tcttcctctt ccagtgatcg accagcatgc ataactcccc cgcagtctcc
    14161 agcgcgaaat cgtttgacct gacctcgacg gcgtttttaa tcgttgcctt tctcaccggt
    14221 attgcgggcg ctctgcaaac cccgacactc agtatttttc ttaccgatga agtacatgcc
    14281 cgtccggcga tggtgggatt cttctttacc ggcagcgctg tcattgggat tctggtaagt
    14341 cagtttctcg ccgggcgctc tgataagcgc ggcgatcgca aatcgctgat tgtcttttgc
    14401 tgcctgttag gcgtgctggc ctgcaccctt tttgcctgga atcgcaacta ctttgttttg
    14461 ctattcgttg gcgtctttct tagcagcttt ggctcgaccg ctaacccgca aatgtttgcc
    14521 cttgcccgtg aacatgccga caaaaccgga cgtgaggcgg tgatgttcag ctctttttta
    14581 cgcgctcagg tttcactggc atgggtcatt ggcccaccgc tggcttatgc gttagcgatg
    14641 ggtttcagct ttacggtaat gtatctgagc gcagcggtag cgtttattgt ttgcggcgtg
    14701 atggtgtggc tgtttttacc gtcgatgcaa aaagagcttc cgctggcgac cggcacggtt
    14761 gaagcgccgc gccgtaaccg tcgcgatacg ctactgctgt ttgtcatttg tacattgatg
    14821 tggggctcga acagcctgta catcatcaac atgccgctat ttattatcaa cgaactgcat
    14881 cttcccgaga aactggccgg tgtgatgatg gggaccgccg ccgggctgga aatcccgacg
    14941 atgttgattg ccggatattt cgccaaacgt ctgggtaagc gtttcttaat gcgcgttgct
    15001 gccgtgggtg gcgtctgttt ttacgcagga atgctgatgg cgcattcacc tgtcattctg
    15061 ttgggcttgc agctgctaaa tgctattttt attggcattc tgggcggcat cgggatgctc
    15121 tattttcagg atctgatgcc cggtcaggcg ggttcagcca ccacgctcta taccaacacg
    15181 tcgcgcgtgg gctggatcat cgcaggatca gtggcgggca tcgtcgccga gatctggaat
    15241 tatcacgctg tgttctggtt tgcgatggtg atgattatcg ccactctgtt ttgcttactg
    15301 cggattaaag atgtttaagg cgcggtcagc atttccagat cgataagcca ggtcatcgcc
    15361 tgctgacgcg agttaccgca catttcagcg ctggcttgta agcctgcaca gactttcggt
    15421 cgcagtggcg aggtgaaaat tttacagcgt tgctgttcat caagctgaat gcagggcgta
    15481 ttggcgggct tgccatctgg cataccggga atagggctgg aaattgaagg ggcagtgcaa
    15541 cacgccccgc aacccggacg gcattccata cgttgttctc ttaaggttaa ccaccggatt
    15601 gatgagcgcg cacagtacca ccttttttgc accagcaaaa gtgcgaatac cacttgcccg
    15661 aaaggcccgt cgcgagtact ttgtcgcgat atttttgaca ttttcgacta caggaatttt
    15721 tcgatgccaa gagcgaacga aattaaaaaa ggtatggtac tgaattacaa cggcaaactg
    15781 ctgctggtta aggatattga tattcagtcg cccactgccc gcggtgccgc tacgctgtac
    15841 aaaatgcgtt tttctgatgt ccgtaccggg ctgaaagtag aagagcgttt caaaggtgat
    15901 gacatcgttg acaccgtgac gctgacccgc cgttacgttg acttctccta tgtcgatggt
    15961 aacgaatatg tctttatgga taaagaagac tataccccgt ataccttcac caaagatcag
    16021 attgaagaag agttgctgtt tatgccagaa ggcggcatgc cggacatgca ggtgctgacc
    16081 tgggacggtc aactgttggc gcttgagctg ccgcagaccg ttgatctgga aatcgttgaa
    16141 accgcaccag gcatcaaagg ggcatctgcc agcgcccgta acaaaccggc gacattgagc
    16201 actggtctgg tgattcaggt accagaatac ttaagcccgg gcgaaaaaat tcgtatccat
    16261 atcgaagaac gccgttatat gggtcgtgct gactaacttc agccgcatgc agaaaaggga
    16321 tagctcaggc tgtccctttt ttaatttatt ataccaatct tctattttgc gcttcctgaa
    16381 caccttacgt cctcaatttt cactcgttga ttgatcgccc tcacacttca tcgcattaac
    16441 aatccagacc aatttcaatt gctgtcatat aactttacac tgtcattgtt aattaatcgt
    16501 tactaagacg tgactcctat gaatacaatc gcctccgtta cgctcccgca tcatgtacac
    16561 gctccacgct acgatcgcca gcagttgcaa tcacgtatcg ttcattttgg ctttggagcc
    16621 tttcaccgcg cacatcaggc gttactgacc gatcgtgtgc tgaatgccca gggcagcgac
    16681 tgggggatct gtgaaatcag cttgttcagc ggtgatcaac tgatgagcca gctccgcgca
    16741 cagaaccatt tatataccgt gctggagaaa ggggcggacg gcaatcaggc gataattgtc
    16801 ggtgccgttc acgaatgcct taatgcaaaa ctggattcct tagcggcaat tattgagaaa
    16861 ttttgcgagc cacaggtggc aattgtttcc ctgacgatta ccgaaaaagg ctattgtatt
    16921 gacccggcca ccggtgcact cgacaccagc aatccgcgga ttattcacga tctacaaaac
    16981 cctgaagaac ctcactccgc gccgggtatt ctcgtcgaag cactgaaacg acgccgtgaa
    17041 cgcggccttg caccgtttac cgtactctcc tgcgacaata ttcccgacaa tggccatgtg
    17101 gtgaaaaacg cggtgctggg gatggcggaa aaacgttcac cagaactcgc cgggtggata
    17161 aaagaacacg tcagttttcc gggaaccatg gtcgaccgta ttgttccggc agcaaccgac
    17221 gaatcactgg cggaaatcag ccagcatctg ggggtgaatg atccctgcgc gattagctgc
    17281 gaaccgttta tccagtgggt ggtggaagat aacttcgtcg ctgggcgtcc tgcctgggaa
    17341 gtcgcaggtg tacaaatggt gaatgatgtc ctgccatggg aagagatgaa actgcgtatg
    17401 cttaatggca gccactcttt tctcgcttat ctgggttacc tctcaggatt cgcccatatc
    17461 agtgattgca tgcaggatcg cgcatttcgc catgccgcca gaacattaat gctggatgag
    17521 caagcgccaa cattgcgaat taaagatgtc gatttaacac aatatgcgga taagttaatt
    17581 gcacgttttg ctaatccggc gctgaaacat aagacctggc aaatcgcgat ggatggcagc
    17641 cagaaattac cgcaacgtat gctggcaggt attcgcatac atctggggcg cgaaacggac
    17701 tggtcgttgc tggcattagg cgttgcaggc tggatgcgtt acgtcagcgg cgttgatgat
    17761 gccggaaatg ccattgatgt tcgcgatccg cttagcgata aaattcgcga acttgttgcg
    17821 ggcagtagca gtgaacaacg cgtaaccgcc ctgctttccc tgcgtgaagt tttcggtgat
    17881 gatctgccag ataacccgca ttttgtgcag gctatcgaac aagcctggca acaaatcgca
    17941 caattcggcg cgcatcaggc gctattaaac accctcaaaa tttaacgatt tctgcggtta
    18001 aagcggatga agctcgcctt cgtccgctcc ccccttctct tttctgcctt ttttagccag
    18061 gattaacgct cagttaactt ttaagagtat tgcggagccg ttgtgaccag gaccaacctc
    18121 atcaccggtt ttctcggcag cgggaaaact acgtcgattc ttcatctgtt agcccataaa
    18181 gatcccaacg aaagatgggc ggtactggtt aatgaatttg gggaagtcgg aattgatggt
    18241 gctttgctcg ccgatagcgg cgcattgctg aaagagatcc ccggcggctg catgtgctgc
    18301 gttaatggtt tacccatgca ggtagggttg aataccttac tgcgtcaggg aaaaccagac
    18361 cgcttgttga tagagccgac cggactgggt catccgaaac agatcctaga tctcttaacc
    18421 gcgcccgtgt atgaaccgtg gatagatctg cgcgccacgt tgtgcattct cgatccacgc
    18481 ctgctgctgg acgaaaaaag cgccagcaat gaaaacttcc gtgaccagct ggctgccgca
    18541 gacattattg tcgccaataa atccgaccgt gcgacgcccg aaagtgagca agcgctacag
    18601 cgctggtggc agcaaaatgg tggcgatcgg caattaattc acagcgagca tgggaaaatt
    18661 gacggtcatc ttctggattt gccgcgtcgc aatttagccg agttgcccgc cagcgccgca
    18721 cattctcatc atcatagcgt gaaaaaaggg ttagcagcgt taagcctgcc agagcatcaa
    18781 cgctggcgtc gcagtctgaa cagcgggcaa ggatatcagg cctgcggctg gatattcgac
    18841 gctgatacgg tattcgacac cattggcatt ctggaatggg cgcgacttgc atcggtagaa
    18901 cgcgtcaaag gcgtgctgcg tattcccgaa gggctggtgc gaatcaaccg tcagggcgat
    18961 gacctgcaca ttgaaacgca aaacattgcg ccaccggaca gccgtattga gctgatttcc
    19021 agcagcgaag ctgactggaa tgccttgcag agcgcgctgt tgaagcttcg tttagcgact
    19081 accgcgtaag gttgcctgcg tttttcagta agataattag agaaaatatg attaaaaatt
    19141 tgccgcaaat agtgttgttg aatattgtcg gcctcgcgct gtttctttcc tggtatattc
    19201 ccgttaatca tggattctgg ttgccgattg atgcggatat tttttatttc tttaatcaga
    19261 aactggtcga aagtaaggcc tttttgtggc tggttgcatt gaccaacaat cgcgctttcg
    19321 acggttgttc tctgctggcg atgggaatgt tgatgctgag tttctggctg aaagaaaacg
    19381 cccctggcag acgacgtatc gtgattattg gtctggtcat gctattaact gcagtggtat
    19441 taaaccagct gggtcaggca ttaattcctg taaaacgggc cagcccaaca ttgactttta
    19501 ccaatattaa ccgcgtcagc gaactgctct ctgttcccac gaaagatgcc tcacgagata
    19561 gctttcccgg cgatcacggc atgatgctgc ttattttttc ggcattcatg tggcgttatt
    19621 tcggcaaagt tgcaggcctt atcgccctta ttatttttgt ggtttttgcg tttcccagag
    19681 taatgattgg cgcacactgg tttactgaca tcattgtagg ttcgatgacc gtgatattga
    19741 tcggtttgcc ctgggtgttg ctgacaccat taagtgatcg attaatcacc ttttttgaca
    19801 aatcactacc gggaaaaaac aaacatttcc aaaacaaata actcacagta attaacatca
    19861 tcagggttat ttttatagtg aggataatcc tgatgatgcg caccgtgctt tcatccatcg
    19921 cgcgcaaaaa tcattctcta agtaaatgaa tggattgcat gcgtttcact caatcgtact
    19981 ttaattgacc aatcccgctt attaactttc tgtatcactt tttcttataa aaaatcatgt
    20041 aaaaccgctc gccaagaccg caccaatcgg gtaatctcga actcgttttg cctcggcggt
    20101 agattatcct cacagcatat aattttgtgc gttagtccac agatttggcc ttaaggaatt
    20161 gtttcaacat gcccaggtaa ttagtctcgt gtcgcttggc atttttttat aacgatattt
    20221 gtcgttaagg acttcaaggg aaaacaaaca acatggtcaa atctcaaccg attttgagat
    20281 atatcttgcg cgggatcccc gcgattgcag tagcggttct gctttctgca tgtagtgcaa
    20341 ataacaccgc aaagaatatg catcctgaga cacgtgcagt gggtagtgaa acatcatcac
    20401 tgcaagcttc tcaggatgaa tttgaaaacc tggttcgtaa tgtcgacgta aaatcgcgaa
    20461 ttatggatca gtatgctgac tggaaaggcg tacgctatcg tctgggcggc agcactaaaa
    20521 aaggtatcga ttgttctggt ttcgtacagc gtacattccg tgagcaattt ggcttagaac
    20581 ttccgcgttc gacttacgaa cagcaggaaa tgggtaaatc tgtttcccgc agtaatttgc
    20641 gtacgggtga tttagttctg ttccgtgccg gttcaacggg acgccatgtc ggtatttata
    20701 tcggcaacaa tcagtttgtc catgcttcca ccagcagtgg tgttattatt tccagcatga
    20761 atgaaccgta ctggaagaag cgttacaacg aagcacgccg ggttctcagc cgcagctaat
    20821 aaaccgtttg gatgcaatcc cttggctatc ctgacgagtt aactgaaagc actgcttagg
    20881 cagtgctttt ttgttttcat tcatcagaga aaatgatgtt tccgcgtctt gatccaggct
    20941 atagtccggt cattgttatc ttttaaatgt tgtcgtaatt tcaggaaatt aacggaatca
    21001 tgttcatacg cgctcccaat tctggacgta agctcctgct tacctgcatt gttgcaggcg
    21061 tgatgattgc gatactggtg agctgccttc agtttttagt ggcctggcat aagcacgaag
    21121 tcaaatacga cacactgatt accgacgtac aaaagtatct cgatacctat tttgccgacc
    21181 tgaaatccac tactgaccgg cttcagccgc tgaccttaga tacctgccag caggctaacc
    21241 ccgaactgac cgcccgcgcg gcgtttagca tgaatgtccg tacgtttgtg ctggtgaaag
    21301 ataaaaaaac attctgttca tctgcgaccg gtgagatgga cattccactc aatgaattga
    21361 ttccggcgct cgacattaat aaaaacgtcg atatggcgat cttacccggc acgccgatgg
    21421 tgccgaacaa acccgcaatc gtcatttggt atcgcaaccc gttgttgaaa aatagtggcg
    21481 tgtttgccgc gctgaatctc aatctgacgc cttcactctt ctatagctca cggcaggaag
    21541 attacgatgg tgtcgccctc attattggta atactgcgct atctaccttt tcgtcacgtt
    21601 tgatgaatgt taatgaatta accgacatgc cggtccgtga aactaaaatt gcgggcattc
    21661 ctctgaccgt tcggctttat gcggatgact ggacatggaa cgatgtgtgg tacgcatttt
    21721 tactgggcgg catgagtgga actgtcgttg gcctgctctg ctattacctg atgagtgtac
    21781 gtatgcgccc cggcagagaa atcatgaccg ccatcaagcg cgaacaattt tacgtggcct
    21841 atcaaccggt ggtggataca caatctttgc gagtaacggg cctggaagta ctgctacgct
    21901 ggcggcatcc tgtcgcggga gaaatacccc cggatgcctt cattaacttt gccgaatcgc
    21961 aaaagatgat tgtgccactg actcagcacc tgtttgagtt gattgcccgc gatgccgcag
    22021 aattagaaaa agtactgccg gtaggcgtca aatttggcat taacattgcg ccggaccatc
    22081 tgcacagcga aagctttaaa gcagatatcc agaaactgct cacttccctg cccgcacacc
    22141 atttccagat tgtgctggaa attaccgagc gcgatatgct gaaagagcaa gaagccacgc
    22201 aactctttgc ctggctacac tcggtcggcg tagaaattgc tattgatgac ttcggtaccg
    22261 ggcacagcgc gcttatctac cttgagcgtt ttacgctcga ttatctgaaa attgaccgtg
    22321 gatttatcaa cgccatcggt acggaaacga tcacttcacc tgtacttgac gcggtgctga
    22381 cgctggcgaa acgcctcaat atgctgacgg ttgctgaagg ggtcgaaacg ccggaacagg
    22441 cgcgatggct aagcgaacgc ggcgttaatt tcatgcaagg ctactggatt agtcgcccgt
    22501 taccgctgga cgattttgtt cgctggctga agaaaccgta tacgccgcag tggtaaggtg
    22561 tgcttacgtc ccttattatt catagagaaa gcatgccgga ttgcggctaa tgatgagtaa
    22621 aaggaaatcc gttgcagatg attgtgcgca tactgctgct gtttatcgct ctgttcacct
    22681 ttggtgcgca ggcgcaggct atcaaggaaa gctatgcctt tgccgtgctg ggcgaacccc
    22741 ggtacgcctt taatttcaac cattttgatt atgtgaaccc cgccgcgcca aaaggggggc
    22801 agataacgtt gtcagccctc ggcaccttcg ataatttcaa ccgctatgca ctgcgcggca
    22861 acccgggcgc acgcaccgag cagctgtacg acacgctatt tacgacttcc gatgacgaac
    22921 caggcagtta ttacccgctg attgctgaaa gtgcacgcta tgctgacgat tattcctggg
    22981 tggaggtcgc tattaatccg ctcgcccgtt ttcatgatgg ttcgcccatt actgcccgcg
    23041 atgtagagtt tacttttcaa aaatttatga ccgaaggcgt gccgcaattt cgtctggtct
    23101 acaaaggcac caccgtcaaa gccattgcgc cgttaaccgt gcgcattgag ttagctaaac
    23161 ccggcaaaga agatatgctg agtctgtttt cgctgccggt gtttccagaa aagtactgga
    23221 agaatcacaa acttagcgac ccgctcgcca cgcctccgct tgccagtggt ccgtaccgaa
    23281 ttacgtcttg gaaaatgggg caaaatattg tctattcccg cgtgaaagat tactgggcag
    23341 caaacttacc ggtaaaccgt ggacgctgga atttcgacac cattcgctac gattattacc
    23401 tcgatgataa tgtcgccttt gaagcgttta aagccggtgc ctttgatttg cgtatggaaa
    23461 acgacgccaa aaactgggcc acgcgctata ccggtaaaaa tttcgataag aaatacatca
    23521 tcaaagatga gcaaaagaac gaatcagccc aggatacgcg ctggctggcg tttaatatcc
    23581 aacgtccggt attcagcgat cgccgggtgc gggaagcgat cactctcgcc tttgactttg
    23641 aatggatgaa caaagcgttg ttttacaatg cctggagtcg cacgaacagt tattttcaga
    23701 ataccgaata cgcggccaga aattaccccg acgccgcgga gctggtgctt ctggcaccaa
    23761 tgaaaaaaga tctaccgcca gaagtcttca cgcaaatcta ccagccgccg gtatccaaag
    23821 gcgatggcta cgatcgtgac aacctgttaa aagccgacaa acttctcaac gaagcgggct
    23881 gggtgctgaa gggtcagcaa cgcgtaaatg ccacaacggg tcagccgctc agctttgagt
    23941 tattgctacc ttcgagcagc aatagtcagt gggtattgcc gttccagcac agcctgcaac
    24001 ggctgggtat caacatggac attcgcaaag tggataactc gcaaatcacc aaccgcatgc
    24061 gcagtcgcga ctatgacatg atgccgcgcg tatggcgggc gatgccgtgg cccagttccg
    24121 atttacagat ttcctggtca tcggaatata tcaattccac ttataatgcc cccggcgtgc
    24181 aaagcccggt tatcgactcg ctgatcaacc aaattattgc cgcgcaggga aataaagaaa
    24241 aattactgcc gttggggcga gcactggatc gcgtattaac gtggaattat tacatgctgc
    24301 caatgtggta tatggcggaa gaccgtctcg cctggtggga taaattctct caacccgcag
    24361 tgcgccctgt ttacagcctg ggtatcgata cctggtggta tgacgttaat aaagcggcca
    24421 aactgccgtc agccagacaa cagggagagt agatgggcgc ttatctgatt cgccgtctgt
    24481 tgctggtgat cccaacgtta tgggcgatta tcactatcaa ctttttcatc gtgcaaattg
    24541 cgcctggcgg tccggtcgac caggctatcg ccgccattga gtttggtaat gccggagtat
    24601 tacccggcgc aggcggtgaa ggtgttcgcg ccagccatgc gcaaacgggc gtcggcaata
    24661 tcagcgacag taattaccgt ggcggacgcg gattagatcc agaagtgatc gccgagatca
    24721 ctcatcgcta cggtttcgat aagccgatcc acgaacgtta cttcaaaatg ctctgggact
    24781 acatccgctt tgattttggc gacagccttt ttcgcagcgc ctcggtactg acgctgatta
    24841 aagatagtct gccggtttcc atcaccctcg gattatggag cacgctgatt atctatctgg
    24901 tgtcgattcc gttaggcatt cgcaaagctg tttataatgg tagccgcttt gacgtctgga
    24961 gtagtgcatt tatcatcatc ggctacgcca ttccagcctt tttgtttgcc atcctgctga
    25021 ttgtcttctt cgcgggcggc agctatttcg acctgttccc tctgcgcggc ctggtttccg
    25081 ctaactttga ttcgctgccg tggtatcaga aaatcaccga ttatctgtgg catatcacgc
    25141 tgccggtgct ggcgacagtg attggcggct ttgcggcgct gaccatgctg acaaaaaact
    25201 cattccttga tgaagtgcgc aagcaatacg tggtgaccgc ccgtgcgaaa ggggtaagtg
    25261 aaaaaaatat tctctggaaa catgtgttcc gcaacgccat gctgctggtg attgccggtt
    25321 ttccggcgac gtttatcagc atgtttttta ccggctcgct gctgattgag gtgatgtttt
    25381 cactcaatgg tcttggctta ctgggctacg aagcgaccgt ctcgcgcgat tatcctgtaa
    25441 tgtttggtac cttgtatatt ttcaccctga ttggcctgct gctgaatatt gtcagtgata
    25501 tcagctatac gctggtcgat ccgcgtatag attttgaggg acgttaatgt cgcgactcag
    25561 ccccgtcaat caggcccgtt gggcgcgttt tcgtcataac cgtcgcggct actggtcgtt
    25621 atggattttc ctcgttttgt ttggtttgag tttgtgttct gaactgatcg ccaacgataa
    25681 accgttgctg gtacgttatg acggcagttg gtatttcccg ttgttgaaaa actacagcga
    25741 aagcgatttt ggcggcccgc tggcaagcca ggctgattat caggacccgt ggctgaaaca
    25801 gcggttggaa gataaaggct gggtactatg ggcaccgatt cgctttggcg ctaccagtat
    25861 caacttttct accgacaagc ccttcccctc cccaccctcc cggcaaaact ggctgggaac
    25921 ggatgccaat ggcggcgatg tgctggcacg tattctctat ggcacgcgga tctcagttct
    25981 gtttggcctg atgctgaccc tctgctccag cgtgatgggc gtgctggcgg gggcgctaca
    26041 aggctattac ggcggtaaag tcgatctctg ggggcaacgt tttattgaag tatggtcggg
    26101 gatgccgacg ctgtttttga ttattttact ttccagcgtc gtacagccta acttctggtg
    26161 gctgttggca attaccgtct tgtttggctg gatgagtctg gtcggcgtgg tgcgggcgga
    26221 gtttttacgt acccgtaatt tcgactacat ccgcgcggcg caggcgcttg gcgtcagcga
    26281 tcgcagtatc atcctgcgtc atatgttgcc aaatgccatg gtcgctaccc tcaccttttt
    26341 accgtttatt ttatgtagtt cgatcaccac cctgacctca ctcgatttcc tcggcttcgg
    26401 tctgccgctc ggttcaccgt cactcggcga actgctgtta caagggaaaa ataaccttca
    26461 ggccccgtgg cttgggatca ccgccttctt gtcggtggcg atattgttgt ctttgctgat
    26521 ctttattggt gaagccgtcc gcgacgcatt tgatcctaat aaggcggtgt agcatgacgc
    26581 aaactctgtt agcgattgaa aatttgtcgg tgggttttcg ccatcagcaa accgtacgta
    26641 cagtagtcaa tgatgtttca cttcagattg aggctggcga aacgctggcg ctggtgggtg
    26701 agtcaggttc aggcaaaagc gttaccgcgc tgtcaatttt acgcctgctc ccttccccgc
    26761 cggttgaata tctctccggc gatattcgtt ttcatggcga atcgctgctt catgccagcg
    26821 atcaaacgtt gcgcggtgta cgcggtaata agatcgccat gatttttcag gagccgatgg
    26881 tgtcattaaa tccattgcat accctggaaa aacagcttta tgaagtgctt tcactccacc
    26941 gcgggatgcg tcgggaagcg gctcgtggcg aaattcttaa ctgccttgat cgcgtcggta
    27001 tccgccaggc ggcaaaacgg ctgacagatt atccgcatca gctctccggc ggcgaacgcc
    27061 agcgggtgat gattgcgatg gcgctgttaa cgcgaccgga attattaatt gccgatgaac
    27121 caaccaccgc actggacgtc tctgttcagg cgcaaatttt acagctgttg cgcgaactgc
    27181 aaggcgagct gaatatgggt atgctgttta ttactcataa cctcagcatt gtcagaaaac
    27241 tggcccatcg cgtggcggta atgcaaaacg gtcgctgtgt cgagcaaaat aacgccgcta
    27301 cgctatttgc ctcacccact catccttaca cacaaaagct actcaacagt gaaccgtcag
    27361 gcgacccggt accattgcca gaacctgcct caacgttgct ggatgttgaa cagcttcagg
    27421 ttgccttccc cattcgcaaa gggattttga agcgcattgt ggatcataat gtggtggtga
    27481 aaaacatcag ttttacgcta cgagcgggtg aaacactggg tttagtaggc gagtcaggtt
    27541 ccgggaaaag cacgacggga ctggcgctgc tgcgactgat taactctcag ggcagcatta
    27601 tctttgacgg tcagccactg caaaatttaa atcgtcgcca gctgttacct attcgtcatc
    27661 gcattcaggt ggtatttcag gatccaaact cctcgctcaa cccacgactc aacgttttgc
    27721 agattattga ggaaggctta cgggttcacc agccgacgct ttctgccgca caacgcgaac
    27781 aacaagtgat agccgtgatg catgaagtgg gattagatcc tgaaacacgc caccgttatc
    27841 cggcggagtt ctctggtggt cagcggcaac gtattgcgat tgccagggcg ttaattctta
    27901 agccctcgct gatcattctt gatgaaccaa catcatcgct cgacaaaacg gtacaggcgc
    27961 aaatattgac gctattgaaa tcattgcaac aaaagcatca actggcctat ttgtttatca
    28021 gtcacgattt gcacgttgtc cgcgcgttat gtcatcaggt tatcgtactg cgacaagggg
    28081 aagtagtgga acaaggaccg tgcgcgcgcg tgtttgccgc accgcagcag gagtatacgc
    28141 gtcagctact ggcgttgagc tgacgcttaa aaaggattgt agtttgagaa gggttcggca
    28201 attgccacac caaaattttt caatcggcat gttgcagcaa actcgtcctg gcgatttaca
    28261 aacaggcacg gctcaccttc acactccagt accgaacatg gaacttcgat gtcgcttaac
    28321 gcctggctga gtttgtacat caccgaccat gcattatcac catccgggct aagcaattta
    28381 agcgccacca ggctgttgtc accgcacggt ccattttgcg gaatcggttc aaccttcgaa
    28441 cctgcgaaac cagcagacca gcgatagttc tgcggcagtc gatggacgat tgagagttgt
    28501 aatgaagtca cttattttcc ccggaagcac atttacttca caatttgttt acatcaattt
    28561 taacacatca tcaacaatcc gtctttatac agatcgtaaa gattgatgcc tcgccagcgt
    28621 ggcctctatg gctctgattt aaataatatc tgcgggcgtg gtcacgtttg cggggctata
    28681 tgtacccgtt tctgcgatct aactcaacct ttttaactac aatgatgtga ctttttacat
    28741 aaattgattt tacataaaat aaacatatat cgggggaatg atggatttgt ggttgacatg
    28801 catcaacaaa ggaagccttt tagcttcctc gttgtgcaat agatcaccgt tttttcggcc
    28861 gactggcgta cagacagaag agaatggagc tggttgcgca gaatgcaatt gaccaaatca
    28921 tcggccatgc agagttaaag gtcgcaagag aaagtaatgc gccaacaatt gcccctatgc
    28981 cgaaacggaa ggttcctgcc agcgaagatg ccgttcccgc catatgggga aactcatcaa
    29041 gaatgaccgc catcgcatta gatgacacca tcgacacgca gcccacaaac gccgcaacgc
    29101 caaccaccag cgaccagaat cccagcccca gcagcgcact gatgaccatc cacgccgcca
    29161 taataaactg tatccacaac cccgagcgga acatatttaa cgcgccaatt cggcggacga
    29221 agcggctgtt aaagatggtc atcacgaaca ggaaaacaat gtttagcgca aagtaataac
    29281 caaagttttc cggcgcgacg tggttaattt caatatagac aaacggtccg gcgctcagga
    29341 acgagaacat cccggcaaag ctgaaaccgc tggccagcat ataactcagg acacgcttat
    29401 gacggaacag cgccgcaaag ttaccaatag tggtacgaat gtgaaaaggc tggcgacgct
    29461 ccggtggtaa ggtttctttg atcagaaaga agatcattgc cgaagccaga atcgccgcca
    29521 cggcgaggat ccagaagatg taatgccagc tcagccacac cagtacccag ccgccaacta
    29581 tcggtgccat cagcggtgca atggtcgtca ccagcatgac aaacgacatc atccgcgaga
    29641 actcttcttt cgggtagata tcacgcatta aagcgttgat aaccacgctg gccgcagcag
    29701 ccgccagtcc gtggaagaaa cgcatcacaa tcagctgctc gatagtttgt gccaacgcac
    29761 acgccaccgc ggcggcggca aacaccagcg taccgccaag aaccaccggc ttacgcccga
    29821 agctgtctgc catcggcccg tagattaact gccccaacgc aaagcccaga atataagtac
    29881 tgagggtcat ctgcgtactg cccgccggta cgccaaactg agctgaaatt accggtagcg
    29941 caggcagata catatcaatc gacagcggca tcaacatggc cagcaggcca aggataaaaa
    30001 caatagcaaa cgacgaatgc tgtcgggtgg tcacaacggg ctcctgaaag tcattgaaaa
    30061 gttagacgac gctggcaatt tcttcttcag ttaacggacg atattcaccg ggggctaaat
    30121 cagcatccag cgtaatgccg ccaatacgtt cacgatgcag ctcaaccaca tggttaccca
    30181 cggcggcgaa catacgtttc acctgatgat aacgcccttc gctgatggtc agacgaacct
    30241 gcgttggggt aatcacttcc agcaccgccg gcttagtgag atctttttcg ttatgcagct
    30301 gcacgccttt agcaaattgc tctgccgtat cgtcagctac gggtgattcc agcgtcacca
    30361 gataggtctt ctcgcaatga tggcgcgggg aagtaatgcg gtgcgaccac tgaccatcat
    30421 ctgtcattag taccagaccg gtggtatcga tatccagacg ccctgcggcg tgcagtttcc
    30481 acgctaccgg ttcatccaga aaatagagca ccgttgggtg atccgggtcg tccgtggagc
    30541 aaacatagcc ctgaggctta ttgagcatga agtaacgtgg accgtgttgc tgcgccagcg
    30601 ggttgccatc gtaagcgaca tcatgttcag ggagcagttt gaacgctgca ttacggacga
    30661 tttcgccatc gaccgtgaca cgattgccgc ggatttcacg cccggcaata gcacggctaa
    30721 cgccgagttg ctgtgcgata aatttatcaa gtcgcatgtg tgtgattttg cctgtaaaaa
    30781 acggaggtcg ggtaattacc cgaaaatctg aactgttgtc tgcccagtat agcggtctaa
    30841 taacttccct caagggaaaa agatccgtgg catactatta gcagaataat ctacctacgc
    30901 gagaccatga tttttacact ccgcccatat cagcaagaag ccgtggatgc cacgctcaac
    30961 cattttcgtc gtcataaaac ccctgccgtc atcgtgctgc ccaccggtgc aggtaaaagc
    31021 ctggtgatag cggaactggc gcggctggct cgtggtcgcg tgctggtgct ggcacacgtt
    31081 aaagaactgg tggcgcaaaa ccatgcaaaa tatcaggcgc tggggctgga agccgatatt
    31141 tttgccgccg ggctaaagcg caaagagagc cacggtaaag tggtatttgg cagcgtgcag
    31201 tcggtcgccc gtaatcttga tgcctttcag ggtgaattct cgctgttgat tgtcgatgaa
    31261 tgtcaccgta ttggtgacga tgaagagagc cagtatcagc aaatcctcac tcacctgacc
    31321 aaagtgaatc cccacttacg cctgctgggg ctgactgcca cgccttttcg actgggcaaa
    31381 ggctggattt atcagttcca ttatcacggc atggtacgcg gggatgagaa agcccttttc
    31441 cgtgactgca tttatgagct gccgctgcgt tatatgatta aacacggcta tctgacgccg
    31501 ccagaacggc tggatatgcc ggtagtgcaa tacgatttca gccgcttgca ggcacagagt
    31561 aacgggcttt tcagcgaagc cgatctcaac cgcgagctga aaaaacagca acgtattacc
    31621 ccgcacatca tcagccagat tatggagttc gccgaaaagc gtaaaggagt gatgattttc
    31681 gccgccaccg ttgaacacgc caaggagata gtgggactgc tgcctgccga agatgcagca
    31741 ctgattactg gcgacacacc cggcgctgag cgcgatgtgt taattgaaga ttttaaagcc
    31801 cagcgttttc gctatctggt caacgtcgcg gtactgacca ctggatttga cgccccgcac
    31861 gtcgatctta ttgctattct gcgccctacc gaatcggtga gtctttacca acaaattgtc
    31921 gggcgcggtc tgcgtctcgc tccaggcaag actgattgct taattcttga ttatgcgggt
    31981 aaccctcacg atctctacgc gccggaagtt ggtacaccga aaggcaaaag tgacaacgtt
    32041 ccggtacagg ttttctgccc tgcctgcggt tttgccaaca ccttttgggg gaaaacgacc
    32101 gccgacggga cattgattga acactttggt cgccgctgtc agggatggtt tgaagatgac
    32161 gacggtcatc gagaacagtg tgatttccgt ttccgtttta aaaattgccc gcaatgtaac
    32221 gcggaaaacg atattgccgc ccgccgctgc cgcgaatgtg acaccgtact ggttgatccg
    32281 gatgatatgt taaaagcggc gctacgactg aaagacgcgc tggtattacg ctgtagcggc
    32341 atgtctttgc agcatgggca cgacgagaaa ggcgaatggt tgaaaatcac ctattacgat
    32401 gaagacggcg cggatgtgag tgagcgtttc cgtctgcaaa cgcccgctca gcgtaccgcc
    32461 ttcgagcagc tttttatccg cccgcatacg cgcacaccgg gcatccctct gcgctggatc
    32521 accgccgccg atatcctcgc ccagcaagcc ttactgcgac acccggattt tgtcgtcgcc
    32581 cgcatgaaag gtcagtactg gcaagtgcgt gaaaaagtgt tcgattacga aggtcgtttt
    32641 cgtcgggcgc acgaattacg cggttaatgg tagttttgat tgatgtataa ggcgattgag
    32701 tatagaatct cgcccgcttt tgcatacgca aagcagatca cttacctgtt gctgggtcgc
    32761 ctgtagcagg attaatttaa gagagaaaga aatgtttact atcaacgcag aagtacgtaa
    32821 agagcagggt aagggtgcga gccgccgcct gcgtgccgct aacaagttcc cggcaatcat
    32881 ctacggtggc aaagaagcgc cgctggctgt cgaactggat cacgacaaag tcatgaacat
    32941 gcaagttaaa gctgaattct acagcgaagt tctgaccatc gttgttgacg gtaaagaaat
    33001 caaagttaaa gctcaggacg tacagcgtca cccgtacaaa ccgaagctgc tgcacatcga
    33061 cttcgttcgc gcttaattgc tgaataagtt gtaaaaaacc ctgctccggc ggggtttttt
    33121 gtctctgcaa attatgcctg attacggtat tgctattttt tgcaggccag ataaggcgtt
    33181 cacgccgcat ctggcattca gaacacaaaa ccgattaatt gccgccggac gtccggcgct
    33241 gcaattgatc gcgcaggttc ggcggcgtgc ctttaatagt cagggtatcg gtcgccggat
    33301 cccagaaaat ccgttcgccc aacagcatcg catcaaagtt aatagtcaag ccaccgccgc
    33361 taccggcaaa cttcgtcaac tgacgcagtg tgctacggtc tgccgggaag ctctcttcca
    33421 gttcgtagcc tttttcagcc gcaaactccg tgaagctcac ttcgctgacg cctgccagct
    33481 ctttcgacag cgattccagt tcaatctctt cacctgcctg tagctgctca ttacagtagc
    33541 tataaacctg ctggcgcacg ttctgtcgct ctgctttatc cagttgtgct tcggcagtga
    33601 aatcatcgac cgcttgcaac agcccacggt tttgcgcttt cgcgtttaac ccttcgctgg
    33661 caccgaggaa atccataaag aaatctgcca ctttgcgccc tactcgccct ttcaggaaag
    33721 tgagatagcg ggtcgactct ggattggttt cccattcggt taaatcgata cgcgcaacaa
    33781 tatcggcatg attgatatca agataatggg ttggattgat gtcgagattt tcgttaacgc
    33841 gcatactgct caggttgctc aggactgcca ccagcagata ctccaccgcc agatagcgat
    33901 agtggcaaaa cagcacgaat ccaccgtcag caaaaggata tttcgctaat tcgtcacgca
    33961 ggcgaccggt tgccgcgcgg ctaaatgcca gaaagtcctc ttcgccctga cgctgtaagc
    34021 gcagcgtctg cgccagttca ctctcttcgc taaacaagcc gtaagcttta tttttggcgc
    34081 tatagacccg atgcagttct gccaccatct caacgacggt ttccgtcggt ttcagcaatg
    34141 aatcgcgcaa caccagctca aggttctgct catcacgctt gataagctgg tgcagagcaa
    34201 tctggttgat atccagactc atgataaact ctcctttaag accgggcggt attcaaccac
    34261 cgccggttaa gcgacgcaat aaaagatggc ggtcatttta cagaaggcag cgtaacgaga
    34321 atgatttaac gcataaaaaa gcagaaaaaa aaccgttgct acggtaatat gttgcccttt
    34381 catcaactaa ctgatttcga tttatgccac aaatttcccg ctacagtgat gaacaggttg
    34441 aacaattgct cgcagagctg ctcaacgtac tggaaaaaca taaggctccg accgaccttt
    34501 ccctgatggt gttaggcaac atggtcacta accttatcaa caccagcatt gccccggccc
    34561 aacgccaggc aattgccaac tcttttgccc gcgccttaca gtcctctatc aacgaagaca
    34621 aagcgcacta agggaaacag ataacaggtt atggtaactc atcgtcagcg ctaccgtgaa
    34681 aaagtctccc agatggtcag ttgggggcac tggtttgcac tgttcaatat tctgctttcg
    34741 ctcgtcattg gcagccgtta cctgtttatc gccgactggc cgacaacgct tgctggtcgc
    34801 atttattcct acgtaagcat tatcggccat ttcagcttcc tggtgttcgc cacctacttg
    34861 ctgatcctct tcccgctgac ctttatcgtc ggctcccaga ggctgatgag gtttttgtcc
    34921 gtcattctgg caacggcggg aatgacgcta ttactgatcg atagcgaagt ctttactcgt
    34981 ttccatctcc atcttaatcc catcgtctgg cagctggtta tcaacccaga cgaaaatgag
    35041 atggcgcgcg actggcagct gatgttcatc agcgtgccgg ttattttatt gcttgaactg
    35101 gtgtttgcga cgtggagctg gcaaaagcta cgcagcctga cgcgtcgtcg gcgcttcgcg
    35161 cgcccgctgg ccgcattctt atttatcgcc tttatcgcct cgcatgtggt gtatatctgg
    35221 gccgatgcca acttctatcg cccgatcacc atgcagcgcg ctaacctgcc tctttcgtac
    35281 ccgatgacgg cgcgacgttt tcttgagaag catggcctgc ttgatgcgca ggaatatcaa
    35341 cgccgtctta ttgagcaagg taatccagac gccgtttccg ttcagtatcc gttaagcgaa
    35401 ctgcgctatc gcgatatggg caccgggcag aatgtgctgt tgattactgt cgatggcctg
    35461 aactactcac gcttcgagaa gcagatgcct gcgctggcag gttttgctga gcaaaatatt
    35521 tcgttcacgc gccatatgag ctccggcaac actacagaca acggcatctt tggcctgttc
    35581 tatggcatct cgccgagcta tatggatggc attctgtcga cccgtacgcc tgcggcgtta
    35641 attactgcgc ttaatcagca aggatatcag ctggggttat tctcgtcaga tggctttacc
    35701 agcccgctgt atcgccaggc attgttgtca gatttctcga tgccaagcgt acgtacccaa
    35761 tccgacgagc agaccgccac gcagtggatc aactggctgg gccgctacgc acaagaagat
    35821 aaccgctggt tctcgtgggt ttctttcaat ggcactaaca ttgacgacag caatcagcag
    35881 gcatttgcac ggaaatatag ccgggcggca ggcaatgtcg atgaccagat caaccgcgtg
    35941 ctcaatgcac tgcgtgattc tggcaaactg gacaatacgg tggtaattat cactgccggt
    36001 cggggtattc cgctgagcga agaggaagaa acctttgact ggtcccacgg tcatctgcag
    36061 gtaccattag tgattcactg gccaggtacg ccggcgcagc gtattaatgc tctgactgat
    36121 cataccgatc tgatgacgac gctgatgcaa cgcctgctac atgtcagcac acctgccagc
    36181 gaatattcgc aaggtcagga tttgttcaac cctcaacgcc gtcattactg ggttaccgcc
    36241 gcggataacg atacgctggc aattaccacc ccgaaaaaga cgctggtgct gaacaataac
    36301 ggtaaatacc gtacttacaa cttacgtggt gaaagagtga aagatgaaaa accacagtta
    36361 agtttgttat tgcaagtact gacagacgag aagcgtttta tcgctaactg attaattata
    36421 aatcagttag caaaatatct tacttgcaat cggtgtggaa aacggtagta ttagcagcca
    36481 cgagtcggca cgtagcgcag cctggtagcg caccgtcatg gggtgtcggg ggtcggaggt
    36541 tcaaatcctc tcgtgccgac caaaaatccc aaaaaaaacc aacctttacg gttggttttt
    36601 ttatatctgc atttaattc
//
LOCUS       DAPOZO010000049        35121 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPOZO010000049 DAPOZO010000000
VERSION     DAPOZO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.049"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..496)
                     /gene="sfmF"
                     /locus_tag="ROI21_002559"
     CDS             complement(<1..496)
                     /gene="sfmF"
                     /locus_tag="ROI21_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDX5111470.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAVDPLGTININLHGNVVDFSCTVNT
                     ADKDKTVDLGRWPTTQLLSAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVA
                     KADAI"
     gene            complement(507..1514)
                     /gene="fimH"
                     /locus_tag="ROI21_002560"
     CDS             complement(507..1514)
                     /gene="fimH"
                     /locus_tag="ROI21_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDX5111471.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPNDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(1527..4136)
                     /locus_tag="ROI21_002561"
     CDS             complement(1527..4136)
                     /locus_tag="ROI21_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDX5111472.1"
                     /translation="MKIPTTTDIPQRYSWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTVNTGDKSGGLMP
                     CFNQALLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQATYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(4167..4859)
                     /gene="fimC"
                     /locus_tag="ROI21_002562"
     CDS             complement(4167..4859)
                     /gene="fimC"
                     /locus_tag="ROI21_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDX5111473.1"
                     /translation="MMTKIKLLMLIIFYLIISTSAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMVAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(5079..5621)
                     /gene="fimA"
                     /locus_tag="ROI21_002563"
     CDS             complement(5079..5621)
                     /gene="fimA"
                     /locus_tag="ROI21_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDX5111474.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            6092..6958
                     /gene="folD"
                     /locus_tag="ROI21_002564"
     CDS             6092..6958
                     /gene="folD"
                     /locus_tag="ROI21_002564"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDX5111475.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            6960..7172
                     /gene="ybcJ"
                     /locus_tag="ROI21_002565"
     CDS             6960..7172
                     /gene="ybcJ"
                     /locus_tag="ROI21_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDX5111476.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQIVA"
     gene            7280..7801
                     /locus_tag="ROI21_002566"
     CDS             7280..7801
                     /locus_tag="ROI21_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDX5111477.1"
                     /translation="MPTIITHAAVPLCIALGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDAVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYSTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(7837..9222)
                     /gene="cysS"
                     /locus_tag="ROI21_002567"
     CDS             complement(7837..9222)
                     /gene="cysS"
                     /locus_tag="ROI21_002567"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDX5111478.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMVAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            9396..9890
                     /gene="ppiB"
                     /locus_tag="ROI21_002568"
     CDS             9396..9890
                     /gene="ppiB"
                     /locus_tag="ROI21_002568"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDX5111479.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            9893..10615
                     /gene="lpxH"
                     /locus_tag="ROI21_002569"
     CDS             9893..10615
                     /gene="lpxH"
                     /locus_tag="ROI21_002569"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDX5111480.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            10732..11241
                     /gene="purE"
                     /locus_tag="ROI21_002570"
     CDS             10732..11241
                     /gene="purE"
                     /locus_tag="ROI21_002570"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDX5111481.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            11238..12305
                     /gene="purK"
                     /locus_tag="ROI21_002571"
     CDS             11238..12305
                     /gene="purK"
                     /locus_tag="ROI21_002571"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDX5111482.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFKRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTAALEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(12458..13351)
                     /locus_tag="ROI21_002572"
     CDS             complement(12458..13351)
                     /locus_tag="ROI21_002572"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDX5111483.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALACLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVMAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(13348..14163)
                     /locus_tag="ROI21_002573"
     CDS             complement(13348..14163)
                     /locus_tag="ROI21_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDX5111484.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGICLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLTGMLLAAWYYGALDARSGRPFFACSDHLQLVTTAVSVNYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(14174..15433)
                     /locus_tag="ROI21_002574"
     CDS             complement(14174..15433)
                     /locus_tag="ROI21_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDX5111485.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     TVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(15443..17110)
                     /locus_tag="ROI21_002575"
     CDS             complement(15443..17110)
                     /locus_tag="ROI21_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDX5111486.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLETTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRARDKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            17427..18476
                     /gene="allD"
                     /locus_tag="ROI21_002576"
                     /pseudo
     CDS             17427..18476
                     /gene="allD"
                     /locus_tag="ROI21_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
     gene            18498..19733
                     /gene="allC"
                     /locus_tag="ROI21_002577"
     CDS             18498..19733
                     /gene="allC"
                     /locus_tag="ROI21_002577"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDX5111487.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VTTLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWKK"
     gene            19744..20529
                     /gene="allE"
                     /locus_tag="ROI21_002578"
     CDS             19744..20529
                     /gene="allE"
                     /locus_tag="ROI21_002578"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDX5111488.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEDKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(20659..21804)
                     /gene="glxK"
                     /locus_tag="ROI21_002579"
     CDS             complement(20659..21804)
                     /gene="glxK"
                     /locus_tag="ROI21_002579"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDX5111489.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTVFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPIGEKVNAFYGLTGDGKTVVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGPRGAAAVFGPQKGATPEM
                     VEELEQGLQNFARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(21826..23127)
                     /locus_tag="ROI21_002580"
     CDS             complement(21826..23127)
                     /locus_tag="ROI21_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDX5111490.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPSFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(23184..24545)
                     /gene="allB"
                     /locus_tag="ROI21_002581"
     CDS             complement(23184..24545)
                     /gene="allB"
                     /locus_tag="ROI21_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDX5111491.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKSEGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHICHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADVFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARIMKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(24606..26060)
                     /locus_tag="ROI21_002582"
     CDS             complement(24606..26060)
                     /locus_tag="ROI21_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDX5111492.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKVTG"
     gene            complement(26230..27108)
                     /gene="glxR"
                     /locus_tag="ROI21_002583"
     CDS             complement(26230..27108)
                     /gene="glxR"
                     /locus_tag="ROI21_002583"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDX5111493.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARLVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(27208..27984)
                     /gene="hyi"
                     /locus_tag="ROI21_002584"
     CDS             complement(27208..27984)
                     /gene="hyi"
                     /locus_tag="ROI21_002584"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDX5111494.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(27997..29778)
                     /gene="gcl"
                     /locus_tag="ROI21_002585"
     CDS             complement(27997..29778)
                     /gene="gcl"
                     /locus_tag="ROI21_002585"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDX5111495.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAVDAPTETCFMHYE"
     gene            complement(29868..30683)
                     /gene="allR"
                     /locus_tag="ROI21_002586"
     CDS             complement(29868..30683)
                     /gene="allR"
                     /locus_tag="ROI21_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDX5111496.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(30761..31243)
                     /gene="allA"
                     /locus_tag="ROI21_002587"
     CDS             complement(30761..31243)
                     /gene="allA"
                     /locus_tag="ROI21_002587"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDX5111497.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            31473..32399
                     /gene="allS"
                     /locus_tag="ROI21_002588"
     CDS             31473..32399
                     /gene="allS"
                     /locus_tag="ROI21_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDX5111498.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            32468..33562
                     /gene="mnmH"
                     /locus_tag="ROI21_002589"
     CDS             32468..33562
                     /gene="mnmH"
                     /locus_tag="ROI21_002589"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDX5111499.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQELHLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALATQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            33678..34043
                     /locus_tag="ROI21_002590"
                     /pseudo
     CDS             33678..34043
                     /locus_tag="ROI21_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(34564..35055)
                     /locus_tag="ROI21_002591"
     CDS             complement(34564..35055)
                     /locus_tag="ROI21_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111500.1"
                     /translation="MLAISSNISKMVIFIFAIIIVVFLCVTTYLYLHKDESLVSKHYI
                     NYMAIPESDGVFTWLPDFFPHVAVDISISTNVEDDYFFSYFSLTIDDGGRFKKTLTVR
                     AREQVAKIVSENDPDTKKVWCKYGKIPGQGDSVNLFFVGEINVTHYFITNIGAGLPDA
                     CAE"
BASE COUNT         8940 a         9323 c         8456 g         8402 t
ORIGIN      
        1 atatcgcatc cgctttcgcc acacctggct gaacaccgct ggctaacgct cgataattgg
       61 caaaaaagtt tagtgtgaca ttgccatttg catctacttc ctcagtcgga ctcgcctccc
      121 ccagtgcgag ccgggagcga tcgctattac gtaattcgat ggcgacggtt tgtgccattg
      181 cgggatcatc cagagccagc aggttggcat cagatgccgg cgttcccgta aataaaatcg
      241 caactgaacc cggaggacat ccctccagcc gcaggctaaa agggacgagt gccgtggtat
      301 cgccagcgct cagtaattgt gtcgtaggcc atctgcctaa atctaccgtc ttatccttat
      361 ccgctgtgtt tacggtacag gagaaatcaa caacgttacc gtgcaaattg atattaatcg
      421 ttcctaaagg gtcaactgcc catccactgg aactccacag tagcccgcag aaacagctaa
      481 agagcactct tctcattctc cgtacctcat tgataatcga cgcgtaaata ccccagcgcg
      541 ctaaacggcc cttcggtcgg tttttgaccg gtaatactga taggccaggc gcgaagtgtg
      601 acattggctg ccgcagctgc atccagacgg aaaggaataa cgctattgag atcgttaggc
      661 gtgatcggcg tatcgttctg atcggccaca ataaaaccta aatcctgatt gtccgacacc
      721 atcgcctgac cagaaacggc actggcttcc agacgcattg ttaaataagc ctgcgcagca
      781 acattcgtac atttgatcgc aatgctcttg gtttgcggca tgacaccagc tggtcgattc
      841 cccggccctg ccgcactaaa taacgatgcg ccgatatcac caaaatcaaa ttcaacaatt
      901 tgcccggcat ttaattcgca gttttgcggt acttcaaccc ggccaccaaa actaatggta
      961 taaacaggtg taaccaacgg atcgttaggc gttgatgtca catacacggt aaacatcacc
     1021 tgtctgggga tctccaccat gttaataaag ggacgaataa cctttaaacg aaatatcaaa
     1081 cgagaatcca tcaccccgaa cggaaggttt tgcgaaacgt taggatctgt tcccatacga
     1141 atataatttt gcggagggaa ttgaatatcc atcacgctat caaccagact catcgcacct
     1201 aatagataat catttaattg catatattta taattatcga tagtttcctg aacaataaag
     1261 ttagtgacat aactacggta tgtataattc accagcgtgc cgggtggaca aattgctgac
     1321 acacctaccc agccggattt ttccggtaaa acaacaatct ggcctggctg gttattggtg
     1381 ctattaaaga catttgagag atcatagtgt tcatcttctg ctacgccagt actatttgca
     1441 caaacagtag cccaggatat gtttgccaga cacaaacatg ccattgccaa taataatctg
     1501 cagattatct tcattgataa tttcctttat gagccaggat gtgtgcaaac tgccgaaata
     1561 acagtgacgg cttgctgtaa gctttgcttc ggcaatacat agtgcgcaat gcagcgtgag
     1621 tttgcaccgt ctccccactg aaccagtaat tcaccagaca atggcgcacc gcttaaataa
     1681 acttgcccgt catcacccac catactggtt attccggtac tgttttcccg taccagtgat
     1741 ccaaacggca ccggttttcc gccctgggta acggtaatga gcgcccgcac gcctatgcgg
     1801 gtgtcaaaat tggcacgaac caacgcgcct tgcgtcggca caacactgct aatatttttt
     1861 tcaacatcga tggaattccc catcgtattg gtatcaagcg cgatacggtt ataccgataa
     1921 accgtggcat acggcatcac cgcatagccg cgccagtcgg ttaaaatgcc agtttgattt
     1981 tcaatgcgta caccgcctgc gccaggcgct ttaatcaaaa cattggtatc ccctaaaggc
     2041 tggctcagcg ttatgccatt ttcatgtccg accacaccgc cagacagctg ccagttaacg
     2101 tcatgttgat cgcggtcgta gttatacccg acccccagcg taccgtaagt ggcctgccag
     2161 tttgccgtag cgctgcccgt atacccatta ttcgaggtat cgccctggct tacgtgataa
     2221 ctcaggttgt ggccttccag taaggtccca cctacacctg ccagccagct attttgcccg
     2281 ttgctgttac ggttggcgtt aaaggaggca taagcgcggt cgagcgcatt ttcccgggtg
     2341 tagcgacgtt tggtcaaaac attgaaaggc actgaaacat taagtccgac aatacgttcg
     2401 ttatcgggaa tccctacaga ttcattccac gaaaatgaga gcgaataact gatgccaacc
     2461 cagctgctgg tataccccac ctgataccac gtatctgaat ccgaagtatt ccagtatttt
     2521 tgatgggtac cagaaatata aagcgagcca aagtcattaa gtgattgtga aatatttaac
     2581 tgcaaacggt ctttacggct aaagcgtaaa ttgtggtaat tcacgattat cggttcatcg
     2641 cgatgctcac cgtcataatc gtaatcatat tcgtacccct ccatacgacg atacgcaaca
     2701 tcatcaaggg tataaaaacc ttgtgtcgaa tagcggtaac ccatcaactg aaaattggtg
     2761 ccgaaagtgt tcattgattt cgcatagagg aagcgaatgg aatccccctc atgacgactg
     2821 tcgtcggcta actgactgcg cgcatgcgtt acatccagcg acactgcgcc ccagttcccg
     2881 aggttgcgcc ccagccctaa caaaaaggcg gtgtaatttg cagataattg cgtcccgccg
     2941 taggcagtaa attcctgtgg taaaccgccg agtgccgtgc cctgaaaaaa gaaaggcgac
     3001 gattgctgac tattaccgct acgaaaatcg cccgctgtca ggtcaaattt gaaacgccct
     3061 tcgcgttgta aaattggcac tgttgaatac ggaattgtgt aattctgctg attgccatcg
     3121 cgctcgtcga tggtgacatc cagatcgcca tttgaagatg tcgggtgcaa atctgtaatt
     3181 tcaaaagcgc cgggggaaac atagctttga tagataataa aaccattttg ccgaatcgtt
     3241 agctgggccg ccgtacgggc aatcccacgt accgttgggg caaacccttg ctggctatca
     3301 ggatacatat tatcggaaga gtaaagccgt acaccacgaa atccaacgcc atcgaaaata
     3361 tcacttcctg tattgccgtc tcccattacc agttcacttt tcagcggaat aatggcgcgc
     3421 tgtacccagg tgccaatatt attccactgt tctgaatgat atccatttcc gcgaaaatag
     3481 ttccaggaac cattgttgcg taaacgccac gggccaatat taatcccgct gagctcacta
     3541 aaaaaatagc tatcgttacc attacctctg ttaccggtga aattataatt caggagtaaa
     3601 gcaggaatac cttcatccca ctcttcaggc ggaatgtaac cgtgagcgct actcaacaac
     3661 gctatctgag gaatagtgat gttcaggcgc atcgctgcaa aatcaaagtt aattgtggca
     3721 tcaggtacag ctttcagtaa attaatgcat ttattatttt gctgctgggc taactcgggg
     3781 aatgcactgc tattaaggcc aattcgttca aggagtgcct ggttaaaaca aggcattaac
     3841 ccacctgatt tatcacctgt atttactgtc gtcgattcaa atacgatatc ctgtgaacca
     3901 atgaactcat cattacgcca gagatccacc cgatacacgc ccgcaggttg atgattacct
     3961 ctctcaaagc gcgataaatc agcaacggat gtgccattct ctaataaaaa tgccgggttg
     4021 aaataacttt ccgcatagct taaaaaggag ggtaaaatgg caagagatga ataacaaatt
     4081 ccggccagac accagctata cctctgcgga atatccgtag tagtgggtat tttcataatt
     4141 agctctgata ttgtcagatg attcggttag tttaagttca cttctctgac cggggttact
     4201 gaaccataat cattaacggt ctgaaatttc agctttccct gcactcctga gggtaaggga
     4261 atttgtgaat taactctggg tgcaaccata gcattcccca gtttttggct gcccacttgc
     4321 aggttaacca gggtgacata aaaaggtgat ggattatgaa cattgatata gttaccggat
     4381 cgcgaaaact tgagtgtgtc cggcgcttct gcgggtaatt cttgtagttg aattggacgg
     4441 agaaataact tcatgcgcga taaaatcgcc agttgcaaaa cattcctgcc gttcaatgca
     4501 tttttatcta ccgaagggat cgtcttaaca ttcatccaga acagagactc acgatctgcc
     4561 gccagcggtg gaccggtgta aataatacgc aaggtatttt cgcttttggg ttcactaaca
     4621 aacagtggcg gtgtaatgat gaatgacttt tcttttacac cgctgctgtt ttcaatccac
     4681 gaattgacca gaaagcgctc attggtatgg ctatttctaa tccataccgc agtctgttta
     4741 gcatcagcgg gataaataat acgcgtggca cctaatgcga tccctccggc agcatgggcg
     4801 ctggtcgaaa tgattaaata aaatataatg agcatcaata actttatttt agtcatcata
     4861 gttaatacgc cttgtgtatt cctgacggca aaaataagga gtataaagaa atgattgctt
     4921 tggtttctta caacacatca ttcccttttc atttaataaa gcggcatgat acaaagtgaa
     4981 catgatttcg atttacattg ccaccatacc gcttcagaca accggtacat tactttgtta
     5041 taagacgtga tatataattc aaaacaacgt ggttttgatt attcgtatct cataataaaa
     5101 gtcgcgtcag catttgcttg ccctgctgat gcacttgtac cggtgccttt ataacgtgca
     5161 gaaaaatgaa gaacgttggt cccggggatc aagttctggt tggttgagaa gctattccca
     5221 tcaggtttga gaattgcgga tgtattatca agtatttcaa taccgacacc cgttgctgtt
     5281 gttgtattag tactggatgc aatggccaat aaattatcat tgattgcatc cgcctgacca
     5341 gaaaatgcaa cggcagcatt agcggcaacg acaggatcgc agtcgttcaa ctgtatagtg
     5401 aatggaatta atgcagaggt attaccaaca gcattgaaaa tatcggtacg atattgaccg
     5461 agtgtgacaa cctggtctgc cgagtcagta ttcactgaac aggcagcatt caccagttcg
     5521 ccttcaaagt gaattgtacc gccatctaca acggcagcat aactacccgt agcggcgaat
     5581 agtgcggcgg ccagcgcaga cgaaataaat cttaatttca tatatattcc ttcaatctca
     5641 tttatcgact ccacatccgt atataaccga ttactttatt taagacactg atagtagtaa
     5701 attccttttt atcctctaag aatgtcttaa ttgaaaatat gcactctatt ctaaaaaata
     5761 gagagccccg ttagatgaat acttccgcgc aaaatatatt caacacaaat atagacctga
     5821 agcggtaaat taccaggctg aaaattcttt ttatattgtc aggtatttct taaattatct
     5881 taatccttaa acaaggaaat aaatcagttc cagatttaca acgccatcat ggacgaaaaa
     5941 tgaagctttc aatctcagcg acagtgcgcc tcaccttcgc aagaggtcgc ttcacgcgat
     6001 aaatctgaaa cgaaacctga cagcgcgccc cgcttctgac aaaataggcg catccccttc
     6061 gacctacgta acagatggaa tcctctctct gatggcagca aagattattg acggtaaaac
     6121 gattgcgcag caggtgcgct ctgaagttgc tcaaaaagtt caggcgcgta ttgcagccgg
     6181 actgcgggca ccaggactgg ccgttgtgct ggtgggtagt aaccctgcat cgcaaattta
     6241 tgtcgcaagt aaacgcaagg cttgtgaaga agtcgggttc gtctcccgct cttatgacct
     6301 cccggaaacc accagcgaag cggagctgtt ggagcttatc gatgcgctga atgccgacaa
     6361 caccatcgat ggcattctgg ttcaactgcc gttaccggcg ggtattgata acgtcaaagt
     6421 gctggaacgt attcatccgg acaaagacgt agacggtttc catccttata acgtcggtcg
     6481 cctgtgccag cgcgcgccgc gtctgcgtcc ctgcaccccg cgcggtattg tcacgctgct
     6541 tgagcgttac aacattgata ccttcggcct caacgccgtg gtgattggcg catcgaatat
     6601 cgttggccgc ccgatgagca tggaactgct actggcaggt tgcaccacca ccgtgactca
     6661 ccgtttcact aaaaatctgc gtcaccacgt agaaaatgcc gatctattga tcgttgccgt
     6721 cggtaagcca ggctttattc ccggtgactg gatcaaagaa ggcgcaattg tgattgatgt
     6781 cggcatcaac cgtctggaaa atggcaaagt tgtgggcgac gtcgtgtttg aagacgcggc
     6841 taaacgcgcc tcatacatta cgcctgttcc cggcggcgtt ggcccgatga cggttgccac
     6901 gctgattgaa aacacgctac aggcgtgcgt tgaatatcat gatccacagg atgagtaaca
     6961 tggcgacatt ttctttaggt aaacatccgc acgttgagct gtgcgacttg ctgaaactgg
     7021 aaggctggag cgaaagcggc gcgcaggcga aaatcgcgat tgccgaaggc caggtgaaag
     7081 tcgacggtgc ggttgaaacg cgcaaacgct gcaaaatcgt tgccgggcag acagtgagtt
     7141 ttgccggtca cagcgtacag attgttgcct gatccctttc ccggcctgta acaacaggcc
     7201 ggttctcttt tcagactttt gccctctacg ccccgttcat ccatgctgat agcatcgaca
     7261 ttttgccgaa gcgaccatca tgccaaccat tattacgcac gcagccgttc ccctttgcat
     7321 tgctttaggg ttgggttcga aagtcatccc tccgcgcctg ttatttgccg gaatcatcct
     7381 ggcgatgctg cccgatgccg acgtcttgtc gtttaaattt ggcgttgctt acggcaatgt
     7441 ttttggtcat cgcgggttta cccattcgct ggtgtttgcg tttgttgtcc cgctgttatg
     7501 tgtgctgatt ggacgccgat ggttcagggc tgggctgatt cgctgctggc tgtttttaac
     7561 cgtctcgttg ctatcgcaca gcttgctgga tgcggtaacc actggtggta aaggcgttgg
     7621 ctggctatgg ccgtggtcag atgaacgctt tttcgctccc tggcaggtga ttaaagtcgc
     7681 gccgtttgca ttgtctcgtt acagcacgcc gtacgggcat caggtgatta tttctgaatt
     7741 gatgtgggtg tggttgccgg ggatgctact gatgggaatg ttgtggtggc gcagacgata
     7801 accggatgcg aaaactcgca tccggcaata gcgcaattac ttacgacgcc aggtggtccc
     7861 ttgcgggcca tcttccagca cgatccccat ctcgttaaga cggtcacgcg ccgcatctgc
     7921 cgccgcccag tctttcgctt tacgggcatc cagacgctgt tgaattaacg cttcaatctc
     7981 agccacttcg ctgtcgtctg cctgcgcacc gctttgcagg aacgcttccg gttcttgctc
     8041 cagcaggccc aatacggcgg aaagtttacg cagatgagac gccatcgcgt ttgccgctac
     8101 catatcttct gctttcagac ggtttacttc acgcgccata tcaaacagca cggaataggc
     8161 ttccggggtg ttgaaatcgt cgtccatcgc ttcaataaag cgcgcttcaa acgcttcgcc
     8221 accggcaggc gcaacggttt tatctgtgcc gcgcagcgca gtgtagagac gctccagcgc
     8281 cgcacgcgcc tgcttcaggt tctcttcgct gtagttcagc tggctgcgat agtggcctga
     8341 catcaggaag taacgcacgg tttcagcatc gtagtatttc agcacatcgc gcacggtaaa
     8401 gaagttaccc agcgatttcg acatcttctc gcggtcaacc attaccatcc cggagtgcat
     8461 ccagtagttc acatactgac catcatgggc acaggtggac tgagcgattt cgttctcgtg
     8521 gtgcggaaac atcaggtctg aaccgccgcc gtggatatca aagtggttac ccagctgctt
     8581 gcagttcatt gccgaacatt caatgtgcca gcctggacgg cccgcgcccc acggcgacgg
     8641 ccagctcggt tcgccctctt tcgacatctt ccacagaacg aaatccatcg ggttgcgttt
     8701 gtcgtcgacc acgtcaacgc gcgcgcctgc ctgcagctgg tcgagatcct gacgcgacag
     8761 cacgccatat gtcggatcgg tcggtacgtc aaacatcacg tcgccgttgt ccgccacata
     8821 agcgtgacct ttggcgatca gttgttcagt gagttcaata atttctgcga tatgatgcgt
     8881 cgcgcgcggc tccatatccg ggcgcagaat gttcaaagca tcaaaatctt tgtgcatttc
     8941 ggcgatcatg cgatccacca gcgccacaaa gctttcgcca ttttcattgg cgcgtttgat
     9001 gattttgtcg tcgatatcag taatgttgcg cacatacttc agcttatagc cgaggaaacg
     9061 cagatagcgc gcaaccacgt caaaagcaac aaaggtacgc ccgtgaccga tatgacagag
     9121 atcgtaaacg gtgattccac acacgtacat gccgacttcc ccggcgtgaa taggcttaaa
     9181 ttcctctttt tggcgtgtca gagtattgaa gatttttagc atcgaagatt ccgtttagac
     9241 atgtgtgggt aattgagttg cgtataatac ccatatttcc cgcgcgaatc agcatacatt
     9301 gcgtgatgat ccgatcgtgc ggttatgcta taacaccacc ctatatatga cccgaaccgg
     9361 gttgaagcac caatcaaacg gaacaggatg caaaaatggt tactttccac accaatcacg
     9421 gcgatattgt catcaaaact tttgacgata aagcacctga aacagttaaa aacttcctgg
     9481 actactgccg cgaaggtttt tacaacaaca ccattttcca ccgtgtaatc aacggcttta
     9541 tgattcaggg cggcggtttt gagccgggca tgaagcaaaa aaccaccaaa gacccgatca
     9601 aaaacgaagc caacaacggt ctgaaaaata cccgtggtac gctggcaatg gcccgtactc
     9661 aggcaccgca ctctgcaact gcacagttct tcatcaacgt ggttgataac gacttcctga
     9721 acttctctgg cgaaagcctg caaggttggg gttactgcgt gtttgctgaa gtggttgaag
     9781 gcatggacgt ggtagacaaa atcaaaggtg ttgcaaccgg tcgtagcggt atgcaccagg
     9841 acgtgccaaa agaagacgtt atcattgaaa gcgtgaccgt tagcgagtaa tcgtggcgac
     9901 actctttatt gcagatcttc atctctgcgc ggaagaaccg gcgatcaccg ccggttttct
     9961 gcgtttttta gcgggggaag cccgcaaggc cgacgcgctg tatattcttg gcgatctgtt
    10021 tgaagcgtgg attggcgacg acgatcccaa cccactccat cgccagatgg cggcagcgat
    10081 caaagcggtg tccgattccg gtgttccctg ttatttcatt catggcaacc gtgattttct
    10141 gctcggcaaa cgctttgccc gtgaaagcgg catgacgtta ttgccggaag aaaaggtgct
    10201 cgaactgtat ggtcgccggg tattgattat gcatggcgac acgctgtgca ccgatgacgc
    10261 cggttatcag gcttttcgcg ccaaagtcca caaaccctgg ctgcagacgc tattcctcgc
    10321 tctgccattg tttgtgcgca aacgcattgc cgcgcgaatg cgcgcgaaca gcaaagaagc
    10381 caacagcagt aaatcgctgg cgatcatgga cgttaaccaa aacgcggtgg tcagtgcgat
    10441 ggaaaaacat caggtgcaat ggctgatcca cgggcatacc catcgcccgg cggtgcatga
    10501 acttatcgcc aatcagcaac ctgcttttcg cgtggtactg ggtgcctggc atacggaagg
    10561 ttcaatggtg aaagtcacgg cagatgacgt tgagctgatt cattttccgt tttaaatacc
    10621 cgcaactttg ctgatttcac agccacgcaa ccgttttcct tgctctcttt ccgtgctatt
    10681 ctctgtgccc tctaaagccg agagttgtgc accacaggag ttttaagacg catgtcttcc
    10741 cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca aaagcgactg ggctaccatg
    10801 cagttcgccg ccgaaatctt cgaaatcctg aatgtcccac accacgttga agtggtttct
    10861 gctcaccgca cccccgataa actgttcagc ttcgccgaaa gtgccgaaga aaacggttat
    10921 caggtgatta ttgcgggcgc aggcggcgca gcgcacctgc caggcatgat tgccgccaaa
    10981 acgctggtgc cagtgctggg cgtgccagta cagagcgccg cactgagcgg tgtcgatagc
    11041 ctctactcca tcgtacaaat gccgcgcggc attccggtgg gtacgctggc gattggtaaa
    11101 gctggcgcgg caaacgcggc gttactggca gcacaaattc ttgcgactca tgataaagaa
    11161 ctgcaccagc gtctgaatga ctggcgcaaa gcccagactg acgaagtgct ggaaaaccca
    11221 gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg taacgggcag ttaggccgta
    11281 tgctgcgcca ggcaggcgaa ccgttaggca ttgctgtctg gccagtcggg ctggacgctg
    11341 aaccggcggc ggtgcctttt caacaaagcg tgattaccgc tgagatcgaa cgctggccgg
    11401 aaaccgcatt aacccgcgag ctggcgcgcc atccggcctt tgtgaaccgc gatgtattcc
    11461 cgattattgc tgaccgtctg actcagaagc agcttttcga caagcttcac ctaccgaccg
    11521 caccgtggca gttacttgcc gatcgcagcg agtggcctgc ggtgtttaag cgtttaggtg
    11581 agctggcgat tgttaagcgt cgcactggtg gctatgacgg acgcggtcaa tggcgtttac
    11641 gcgccgatga aaccgaacag ttaccggctg aatgctacgg cgaatgtatt gtcgagcagg
    11701 gcataaactt ctctggcgaa gtgtcgctgg ttggcgcgcg cggctttgat ggtagcaccg
    11761 tgttttatcc gctgacgcat aacctgcatc aggacggtat tttgcgtacc agcgtcgctt
    11821 tcccgcaggc caatgcgcag cagcaagcgc aagccgaaga gatgctgtcg gcgattatgc
    11881 aggagctggg ctatgtgggc gtgatggcga tggagtgctt tgtcaccccg caaggtctgc
    11941 tgatcaacga actggctccg cgtgtgcata acagtggtca ctggacgcaa aacggtgcca
    12001 gcatcagcca gtttgagctg catctgcggg cgattaccga tctgccgtta ccgcaaccag
    12061 tagtgaatag tccgtcggtg atgatcaacc tgattggtag cgatgtgaat tatgactggc
    12121 tgaaattgcc gctggtgcat ctgcactggt acgacaaaga agtccgtccg gggcgtaaag
    12181 tggggcatct gaatttgacc gacagcgaca catcgcgtct gaccgcggcg ctggaagcct
    12241 taatcccgct gctgccgccg gagtatgcca gtggcgtgat ttgggcgcag agtaagttca
    12301 gttaacctgg tgcacaaacc tgcttaaaag ctaatgcgca agcagttttc cccctcaccc
    12361 taaccctctc cccacagggg cgaggggact gtccgcgttc ttatttgggg tttggaatct
    12421 ttctcagagc cttaattctt gccaaatacc ccaatcacta cagcgaaata caggtccccg
    12481 cttcgcccgc cagcgtctct tcaatccgcg ataacgcccc aatccacgca ggtttgccac
    12541 ggcttctgac atagccactc accgccatta ccttcggccc catcgaacca tcggctttgg
    12601 caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
    12661 agttttcata taccgcatca gcatcggtga ggatcaccag cccatcggca ttaatctgct
    12721 cggcgagcaa cgcagcggcg agatctttat caatcactgc ttcactccct gctccgtcat
    12781 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
    12841 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgctacc acccggcgca
    12901 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
    12961 ctggctgata aaccggaccg ataaacttct ctggctgcaa aaacgccgga tcatcaggcg
    13021 aaacctcaat gcgcgtcagc accgtcgtga caggcggcat ctgcggctgt gcgctcaagc
    13081 tctgcgccag catatagcca atcatcccct ggctttccgc caccagcaca tccagcggat
    13141 acggttctac ctctttccac gccagattct gtaacgccaa cagccccacc tgtggcccgt
    13201 tgccgtgaac aatcgccagc cgataagaac gggccaggca cgccagtgcg ggtacagcgc
    13261 tggcgatatt gcgatattga ttttctgccg tcagcgcctc accgcgctga agcaaggcat
    13321 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttgaccaag
    13381 ccagaacccc agcagcgtat ctgcccctga cgtatgtccc agcgccagca gcgaatcaat
    13441 cgcaacagcg gtacgtttcg ggcaactcag agcatgaaca aagtgcagga gtggcgaggc
    13501 gaaatatcct tgcgcggcat aacgtaaata attaacgctc accgcagtgg taacgagttg
    13561 aagatggtcg gaacaggcaa aaaacgggcg accggagcgc gcatctaaag cgccataata
    13621 ccaggctgcc agcagcattc ccgtcagcgt gtcatcatga ctcggcgtta atcccggccc
    13681 tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    13741 acgaaactgg cgtaattcag caggcagcgg atcgcctgcc gccaacgcca gcggcccgaa
    13801 aagcccggtt tcctccgcgc gttgcatcca tgccgctgca agcggttgag gatgcgcagg
    13861 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gtccgaggca
    13921 aatgccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    13981 gaattgcgcg cggcgaagca cccatcctcc ggggccgaaa ccactaccct gacgatgcag
    14041 cgttaacagt tcgccgcttt ccgtcatcag gttaatcgcc cgccgccaca cgcccgctaa
    14101 ccgccaggac tggcgataat tcggagcgct acggctggca agcagaggat ggatgatcgt
    14161 cattgcgcgt tcctcaacca atccccatgc tttcagccag tgcttccagc gcctgttcaa
    14221 agcaggccaa cggtgcccgc acagtgcctg cgccaatctg cccgatcccc gcctctttat
    14281 gggcgatacc ggtattgatg agtggcgtaa tgccggtttc taccacgcga cgaatgtcca
    14341 gtcccaggca cgcgccctgg aaatcccagc cggggatctg caactgcata ttgcgctcaa
    14401 ggtaaatttc cgccatctct tcagaaacgg ttctggccgc ttccatgcca cccgcaccga
    14461 caaagcgcgt tacgccaggc gctgcgatca ttgccgcacc tccgatacca aaggtttcgg
    14521 taattgcgct atcgcccata tccgggttcg cctgctcctg cgagaagccg gtgaaaaaca
    14581 gaccttgggg agtgtttaca ggcgcagtaa accagcgttc gcccagcccg ctgacccgaa
    14641 tcccgaacat attgccgttg cgggtcatag ccgtgacgat gctgcctgca cggatcatcg
    14701 caccagcatc catcgccgcc ttgcagtaag ccatcgcgag gttaaggaag aactgatcgg
    14761 tcacgctgag gaaatccatc acttccgcga tgtgttgttt atcatgatcg aggcgagcaa
    14821 tttgtggggc cagcgcacgc attaacagtg cagaggaagc aatattgcgt tgatgaaact
    14881 catcgcccat ggtaatgccc tgcgccatca tcgccgtgag atcgataccg cgctccatgc
    14941 gccccagcgc cgcacttaat actggcatta acacatcgcg catccagcga tggcgagtca
    15001 ggacatcttc gccgtaagcg ccaaaacgca tcactttgcc gataccttcg ttgaggttgc
    15061 agtacgcccg gttgccgtcg gtcacgttct cgaccaccag catcggcata ctggcagaag
    15121 taatgccgcc cattggcccg acggcattca catggtgaca aggaatgaag ttcacttccc
    15181 cctgctccag tattgccagc gcctgcgcct catctttcgc ccagccttcg aacagacatg
    15241 cgcccacgca cgcccctttc atgggtccgg tcatctcctg ccagcgcatt ggcggcccgg
    15301 cgtgaagcag tgttttgccc ttgtttagtt cgctgataag tgaagaagcc ggttgcacat
    15361 ccagccagtg tggacgagcg cgacgaattt gttcgataac cgcagcattg gcttgcgcca
    15421 ctgatgtaaa catggaaacc ccttattgca aacgttctaa taaacgagcc agttttttat
    15481 tgccgccggc gactggcgac cattggtaat gcacaaccgg tttgctggcg ctttgtagct
    15541 ccagcgcaaa gctgcgtaat ccgatgttaa tcacggcgac gttttccagt aatgacggtg
    15601 tgtgttgctg tgtggcaggc gagagcggat gaattaacgc tgccgccagc aaggtggctt
    15661 ccggcagcga actcacaacc gcgatccccg catcttccag cgtggcgatt tgctgcgagc
    15721 ggcattgcgg gtcacgttcg gtacctgtca ccgtggcaat ggcgtacagc ggttggctgt
    15781 cagaacgcgc ggcacaggct ttttgccagg cgctcaccag cgaggcggca ggatcggcgg
    15841 tcgcaccgaa gccaatcacg acatcaagca gcaacacgcg cacttgcggt ttagcgccga
    15901 gatcggcaat taacaggttg cgtaaggtcg ggtcgatcat cggatgggga cgcccgacgg
    15961 tgtaaaaatc atcgcccagg tcgaggatct gatgtccgtc ggcgtcgagc atcatgccat
    16021 gttgatgggt atcgtcggct tccacgccaa ggtgtccggc aagtaatccc gccgcttcag
    16081 cggccagcgt accgccggta tacaaaccgc aaataaatcc gctgctgaca ggcgctattg
    16141 cgttacgtcg cgccgtaacg cgtgaaagca ggcaagccag gcgtgcggcc tcatccagcg
    16201 aggaggcaaa ccagacattc tcgtcgcggg ctaccgccgg ggtataacct aaaaacagcg
    16261 ccaccgtcgg tttgccagtt gctttcatgg cattaacaat tttcagacgc acagcttcgg
    16321 caggtggttt tgaaacaaat gccagcactt cgcttttctc gtctgcactg agcatttcca
    16381 gcgccgttag cgcactgatg ccgcccactt cacggctgag gtcgcgcccg ccaagaccaa
    16441 tcgcgtgggt aattccctca cctgccagcg caatctgcga acacagctcc tgaatcccgg
    16501 taccggaagc gccaatgacg ccaatgttgc cttccggcat cacgttagca aaagccagcg
    16561 gcgtggcggc aatcatcgac gtaccgcagt ccggccccat caccagcaag cctttatcgc
    16621 gtgcgcgggt tttgagctgg atttcatctt ccagcgtgac gttatcggag aacatcatca
    16681 cgttgaggtt gcgatccagc gcctgattcg ccagttccgc cgcatactcg ccagccactg
    16741 aaatcagcgc cagactggca tcgggtaatt tctgacaggc actgtcccag cgacgcacct
    16801 gcgtcaacgc ctggctgctg cctgaaccct gcgccagttg ttttagcgcc tcttcaagct
    16861 gttgcataat cgcctgcgcg atccccgcat ccgccgcttc gctacgaatt gccacgcaaa
    16921 tatcgttcgg cgtggcgtta ttaaaatcgt catgccagaa acctgtggtc tctaataacg
    16981 ctttattggc gggcgtaccc atcattacgg aaacatcatc aacattttct gattcgctga
    17041 gttttcgtga aataatcatt aaactgaccg aatcctgaaa acaccctttt ttaataaagg
    17101 cgtggatcat aacaactcct taaaaattgc acccatggca attgcacgag cagtgttaat
    17161 gtggcgatta cggtatcgat gacaacgagt aaataaggtg atgaatatca cacagggatt
    17221 attaacaaaa gaattcaaat aaccgccagg ctattacgtg ttatttttta ataagcgtgg
    17281 ttgttttaaa aaccaggtta aaagcgagcg gattatcaaa taaattctaa ctatattttt
    17341 ttgcctgtct ggatcacata atccagatat tttccctgta tgttaattgc agtcatgctt
    17401 cacaccgtcg ttaaaaagga agacagatga aaatcagtcg ggaaacactc caccagctaa
    17461 tcgagaataa actctgccag gctgggttaa aacgtgagca cgctgcaacc gtggctgaag
    17521 tattggttta cgccgatgcc agagggatcc actctcatgg cgcggtgcgc gtggaatact
    17581 acgcggaacg catttcaaaa ggcggcacca accgcgaacc ggagtttcgt cttgaagaaa
    17641 ccgggccatg ttcagcaatt ttacatgctg acaatgccgc cggacaggtc gcggcgaaaa
    17701 tgggtatgga acatgccatc aaaaccgccc agcaaaatgg cgttgcggtg gtcggtatca
    17761 gccggatggg tcacagcggc gcaatctctt attttgtgca gcaggcagcc cgcgccggat
    17821 taattggcat ttcgatgtgc cagtccgatc caatggtggt gccgtttggc ggcgcggaaa
    17881 tttactacgg tactaaccca ctggcctttg ccgcgccggg agaaggcgac gagatcctta
    17941 cctttgatat ggcgactacc gtacaggcat ggggaaaagt gctcgacgcc cgctcgcgta
    18001 atatgtctat cccggatacc taggcggtcg ataaaaacgg agcaccaaca accgatccgt
    18061 tcgcggtaca tgctctgctc cccgccgccg ggccgaaagg gtatggcctg atgatgatga
    18121 ttgacgtcct ctcaggcgtc ttactcggct taccgttcgg gcgacaggtt agttcgatgt
    18181 atgacgattt acacgccggg cgtaatttgg ggcaattaca tatcgttatt aatccgaact
    18241 ttttctcctc cagcgaacta ttccgtcaac atcttagcca gaccatgcgc gaattaaatg
    18301 ccataacccc cgcgcccggt tttaaccagg tttattatcc cggacaggat caggatatta
    18361 aacaacgcaa agccgccgtc gaaggcatcg aaattgttga tgatatttac cagtatttaa
    18421 tttccgacgc gctttataac acgtcatacg aaacgaaaaa tccctttgcg caataattat
    18481 tgagacagga ctttcttatg attacacatt ttcgtcaagc catagaagaa acgctgccct
    18541 ggctttcctc ttttggcgct gacccaacgg gtgggatgac ccgtttactt tattcgccgg
    18601 aatggctgga aactcagcag caatttaaaa aaagaatggc agcaagcggg ctggaaacac
    18661 gtttcgatga agtggggaat ttatatggtc gcctgagtgg caccgaatat ccacaagaag
    18721 tggttctgag cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat
    18781 tcggcgcgct ggcggcgtgg ctggcaattg actggttgaa aacgcaatac ggcgcgccgt
    18841 tacgtacggt cgaagtggtg gcgatggcag aagaagaagg cagccgcttc ccgtatgtct
    18901 tctggggcag taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg
    18961 atgccaaagg aaatagtttt gtcgatgcga tgaaggcttg cggatttact ctgccgaacg
    19021 ccccactaac tccgcgtcag gatattaaag cctttgtcga actgcatatc gaacagggct
    19081 gtgtgctgga aagtaatggg cagtcaattg gcgtggtgaa tgcaattgtc gggcagcgtc
    19141 gttatacggt gacgctgaat ggcgaatcaa accatgcagg caccacgccg atgggttatc
    19201 gtcgtgatac ggtttacgct ttcagtcgca tttgccatca gtctgtcgaa aaagcgaaaa
    19261 agatgggcga tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg aatacggtga
    19321 atgtggtgcc gggtaaaacc acgttcacca ttgattgtcg ccataccgat gctgccgtgc
    19381 tgcgcgattt cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggaca
    19441 ttggtattga tatcgattta tggatggacg aagaacccgt gccgatgaat aaggacctgg
    19501 tcaccaccct gacagaattg tgtgaaagcg aaaaactgaa ttaccgggtg atgcacagtg
    19561 gtgccgggca cgacgcacaa attttcgcgc cgcgcgtgcc gacctgcatg attttcattc
    19621 ccagcatcaa cgggatcagc cataacccgg cggaacgcac caatattacc gaccttgccg
    19681 aaggggtcaa aacgttggca ctcatgcttt atcaacttgc ctggaagaaa taaggagtta
    19741 taaatgggat atttaaataa cgtcaccggt taccgcgatg atttactggc taaccgtgcg
    19801 attgttaaac acggtaattt cgcgctgtta accccggatg gtctggtaaa aaatattatt
    19861 cctggctttg aaaattgtga cgcgacaatc ctctccacgc caaagctggg ggcctctttt
    19921 gttgattatc tggtcacact gcatcaaaac ggtggcaacc aacagggctt cgggggcgaa
    19981 ggcattgaaa cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaagataaa
    20041 acatttgcct taagcgaagg tggctatctt tattgcccac caggctcctt aatgacgttt
    20101 gttaacgccc aggccgaaga cagccaaatc tttttatata agcgtcgcta tgttccggta
    20161 gaaggtcatg cgccgtggct agtttctggc aatgccagcg aactcgaacg cattcattat
    20221 gaaggcatgg acgatgttat tctgctggat tttctgccca aagagttagg ttttgatatg
    20281 aacatgcata tcctctcttt tgcgccaggt gccagccacg gttatatcga aactcacgtc
    20341 caggaacacg gtgcctatat tctttccggt cagggggttt ataacctcga caataactgg
    20401 atcccggtga aaaaaggcga ttacatcttt atgggcgctt attccttaca ggccggttat
    20461 ggcgtagggc gtggtgaagc gtttagttat atttactcaa aagattgcaa ccgcgacgta
    20521 gagatttaag tcataccact cgcctgatta tttttagcta tgttggattt tgccacggtt
    20581 tataccgtgg ctttttttgc ggtaaattca ggtaaaaaac ggatgcggga taaaatgcat
    20641 ccgctctaat ggcaagagtc agtttttaat tccctgacct attttaatgg cgcaggcaat
    20701 atttcgtgcg ctgttgaaga gattggtttc accgctggcg agcacttcgg ctaaaggtgc
    20761 cagacgtggc aaaatgctga acaccgcgtc aatgccgtac tggtgcacca cttccacgcc
    20821 atcacccaat acgccagcaa tcccaatcac cgggacatta aactgcttcg ccaccgacgc
    20881 cacgcccagc ggcgctttac cacctgccgt ttgcgagtcg atgcgccctt ccccggtgat
    20941 caccagcgct gcaccctgca ccgcctgctc caggttgact gcccgcagta caatctcaat
    21001 acccggttta atatccgcat tgaggaatac cgccgccgca atacccatcc cgcccgccgc
    21061 gccgccgccc gccatctggc agacgttgat ttccgtttgc tgttgtaaaa cgcgggcgaa
    21121 attttgcagc ccctgttcca gctcttcgac catttccggc gttgcccctt tttgcggacc
    21181 aaacaccgcc gccgcaccgc gtggccccac cagcgggtta tcaacatcac acgccacttc
    21241 gatatgacaa tttgccagac gcggatcgca ttcatccatc tcaatacttg ccacgcgtgc
    21301 taaattgccg ccgttagccg ccagcacctg accgtcggca tcaaggaaac gcacaccaag
    21361 cgcctgcgcc atgcccatac cgccgtcgac cgtcgcactg ccgccaatgc cgagaataat
    21421 atggcgaatg ccgttatcca gcgcatgacg aatcagctct ccggtgccaa aacttgacgc
    21481 cagcaacgga ttacgctttt caggcgcgac tagcatcagg ccgcttgctg ccgccatctc
    21541 aatcaccacc gttttcccgt cgccggtaag accataaaaa gcattcactt tttcgccaat
    21601 cggcccacag acttcaagcg tcacaatgtt gccgcccgtc gcggcgacca tcgcatccac
    21661 cgttccttcg ccgccatccg ctatcggcaa acagatatag tgcgcatcgg gaaagacggt
    21721 cgaaaaccca gctttaattg cctgacaaca tttttctgca cttaagctct ctttaaaaga
    21781 gtctggcgca atgacaatct tcattatcaa tcctttttca acaaattatt cgatgcgttc
    21841 ccacggaata agattatcca taagaacagc cagtaaaatt ccgaccagta agccgttact
    21901 gagcagagga cgaagcgtta atggaaggtc ttgcagatac acaggcggta atgccattaa
    21961 aaatatgccg acaaataacg gcaatgcgag tcgataaata ttgcgagcgg taaaggttat
    22021 ttgctggcta aacaccagcg ccgaaaagag taaaggcaga taagaaacca gcatgaccgc
    22081 gctactcact ggtaagggga tactgcaaaa cagacgcgtg agtgccggaa ccagcgccac
    22141 cagcaggcaa ataacgctgc cataaataaa cgaacgccgc gtgtaatcgc cagtctgggt
    22201 taataaacca atggatgaaa caaacggtga aaatggaatt accgccagcg gtacggttat
    22261 cagcgtcata aatccggtcg ccacaaagct acgacgataa cgcgtattcc ccgcgccctg
    22321 ctgcggataa aaaacatccg tgccccgaat cgcaccgtag gtattgctga tattaaccag
    22381 acctgtaatc accgccgtca gaataatccc cggcgacaaa gcaccgccac tgccgagcgg
    22441 gaaccactgc cagtgcaact caccggagag cgaatgcgaa gaaggaaagc aaaagtacca
    22501 caacaaccag ccggttatcg taccgaccag caggccataa cgggcaaaac gttgcggcag
    22561 aaagataatc atcgccagta ccaggcacat caccgcgacc gaaagcgcga acgacggtaa
    22621 ctgaatttta aaattcgggt cggctatgcc aaacggcagc ccgagcatac ctttgaaaaa
    22681 gatagtggtc agctgcgcgc ccagcatcaa cataaacaag accatcaccg acggcgtaaa
    22741 taaccgcgcc agacgatgac ctaatccgct aaaaccaatc aacatcgtca gcacgccaga
    22801 gagcgcaatt cccaccgcca ggctggtagc gatatcgttg atcggtgtcc cgcgcgatgc
    22861 ttcgccaagg gtgatagtaa ggatggttcc ccaccacagg ccacccggcc cttccataat
    22921 agcgcgacga tgaccgcaga acgcctgagc gaagcaggcc agtgcagtag caagaaaagc
    22981 atattgcgtg agcgtaagca ggctactttg cggcaactga aaagcagaaa gtagcgtagg
    23041 aggaaccaca accgtgttgc aaaaaataaa gaaaaaccac tgaaatcctg ataacaggct
    23101 ttcgcggctg actgcaaaat tgaacataaa cacaccttaa ccggagaatg cccgccgcaa
    23161 ggacgggcat tgcaggggcc agattactgc tgatgtttaa ggataaattg acctttcggc
    23221 gcaacaggga agccctgttc aatgtcgtaa atcacatcac cacgtaagat ggttttcatg
    23281 atacgcgcgc caatggtacg gccaacatac gggctgactt tgtggcgata ttccagatcg
    23341 tcattggtaa gaacatagct gctattcggc tgaatgaaga cgaagtcggc atcttttcct
    23401 ggggcgatac ggcctttttg ctgcagaccg aaaacatctg ctgcgttagt cgccattaat
    23461 ttgccgaaca tcggcagaga cataccgcgt ttctgtaccg cttcatcgaa catcacgtcc
    23521 atacagtttt gcagaccggc aataccgccc catgcttcca tgatgttgcc ggctttcatt
    23581 tccggcgggc atggcgagtg gtcggaaacc aggcagtcga tttcaccgtt aaacagtttt
    23641 tcccacatgc ctttctggtt ttccagatcg cggatcggcg gtgaacactt tgccagagta
    23701 ccgatttctt cgaactgatc ggtatccagt acaaagtaat gcgggcagga ttcacaagta
    23761 acgtcctgac cttcctgacg tgcacgagtc acttcctcaa cgccttccgg gctgctgata
    23821 tggcaaatgt gcagacggca accggcaact ttcgccaggt acagtacgcg gcgaatcgct
    23881 tccacttcgg taaataccgg acgcgaagcc acatagtcat gggcagttac gcgaccttca
    23941 cttttcgctt cttcgcccag tgcatcacag atcagcgcgt tttcgcagtg caccagcacc
    24001 ggctgcccca gttcgcccag cttctgcgca cctttgaaga actgccagtc gtttacatca
    24061 cggaagtcgt tgtcgatacc gcgatcgcca caggttgcga cgaagcattt gaagccgaca
    24121 acgcccactt catccagctc atgcagacgg tcgatgttgt aagacaccag gccaccgagt
    24181 tgtgccgcat cgatagtcag cttgccttta gcggcatcga acttcagttc aattgaagcg
    24241 cggtcaaccg ttgcaggcag ctggttgagc ggcatttcga tcatggtggt gataccacct
    24301 tttgccgctg cgcgagtacc ggtttcataa ccttcccagt ggctacgacc cggttcagaa
    24361 atatgggtgt gcgcatcaac catgcctggc gaaaccacca gaccagacgc atccataact
    24421 tcttttgcat cgcccagatc ctgaccgata gcagcaattt ttccgccttt aacggcgata
    24481 tctacaacgc gagcttcgtt ttctaaaata acagtgccgt ttttaatgat taaatcaaaa
    24541 gacataacaa actccttgta aaatacagat tggtttttta actcaatgtt caatatcggg
    24601 attaattaac ctgtgacttt ttgttctcct gttttttctg ctgtagtacg tttctttaat
    24661 aaggcgtagg ccgcaaaggc gacgatgacg ccgacaaacc atgaaacacg agataacggt
    24721 tccataaagg gaataaactt accgccaaga gataaaataa cggccaccag agttactgaa
    24781 aacgcagtga ggttaaaacc gttatcgtaa tatttataat cgccaggtgc ggtgtacagt
    24841 tcatcaagat taatttgccc acgcatcacc acaaaataat gcgccatcat gacaccaatt
    24901 accggaccca gcatcccgcc gataatatcg aggaacagat aaatactgtc ctgattttcc
    24961 attaatttcc acgggcagat cagcaggctg ataatactgg caatcagtac gccgttttta
    25021 taggtcagtt tcgtcggtgc aatggcggca atctgataac cagccggaat aatattaccg
    25081 gtggcgttag tagagatagt cgtcatcaga ataaccagta ccgcaaagaa cgaggcaaac
    25141 aggctatccc aacgctgaac aatatccagc acgttccagg tgtcagcgcc gtagtgaata
    25201 ctggctccgg caataataca taccccggcg accgcaaaca gaatataggc cacaactaaa
    25261 ccgagcgttt gccccagcgc ctgctcacga aacgagtggg cattttgcgt aaagtcggat
    25321 gcgctcaccg ccggtgccgc ccagaccgca actaccgcgt taatcaccac caggaacagg
    25381 aagccactgt tttctgcttt ctgaataccg ctcggaatgt agtcaaagat tggaccgagc
    25441 ccgaccagcg aaatcgccca aatcgccata ccgccgaaaa cgatatagat gcacgggtta
    25501 agaatggcag tgaatttatt taaaactttg ccaccgccaa aacctatacc aacgttgacc
    25561 agccagaaga gtaagaaagt aattaagccc ggtagagaaa gccctaacag ggtaaaatca
    25621 ccaccgagag ttaaaaatcc cggccagatt ttgccaatca ggatcaagca ggccagcgat
    25681 ccagcgtaac attgcaggcc aaaccacata atggcagcaa tcccgcctct taataatccg
    25741 ggaaacagtg caccacgtac gccgtaagaa gcacgcagga tcatggcaaa aggcacaccg
    25801 tatttactgc ccgcagcacc gtttaatacc attaccgcgg caatgaaaaa ggcgctgagg
    25861 ataattgcca gcataatact aaaggtagac aagccgagaa taaaaaagcc gccgaccatc
    25921 acataattgg gaacgttatg aaccgaaccc atccataagg taaaatagtt aaatgttttc
    25981 caggtccgct ggctttgcgt tttcggcaat agatcttcgc tatagccgcg ttgctggaat
    26041 agttttctct gatgttccat aaatccctct taatttcacg tatcgaataa cgtcttttta
    26101 atctagctat gaggaacaaa taaaataaaa atattatttt taatactgaa gcaacatctt
    26161 tggctgcttt ctttttttaa tattgctggc cgattcattt tctgatatcg gccattttta
    26221 ttgcgggtat caggccagtt tatggttagc cattaattcc agcgcctgca ctaacgcaga
    26281 gtgatccaac tggctgccac cgtttgccgc acaggtatta aacaattcct ggcaggtcgc
    26341 ggtgtttggc aggttcagcg ccagtgcttt cgcactttgc agtgccaggt tgagatcttt
    26401 ctggtgcagc gcaattttaa aacctggatt aaaggtgcgt ttaatcatgc gctcaccgtg
    26461 aacttccaga atacgtgagg aagcaaagcc gcccatcagc gcctggcgca cacgtaccgg
    26521 atctgcaccg gcttttgaag caaacagcag ggcttcagaa accgcttcaa tattgagcgc
    26581 cacgataatc tgatttgcca ctttgcaggt ttgaccgtca ccgttaccgc ccacgagggt
    26641 gatattttta ccgagcagtt caaacagcgg tttaacacgt tcaaataccg cttcgtcacc
    26701 gccaaccata atcgacaacg tcccttcacg cgcgccgatt tcaccgccgg agactggcgc
    26761 atcgagataa tcgccgccca gttcattcac ctgacgagcg aaacgcttag tttcaatcgg
    26821 ggaaatggag ctcatatcaa caatggtttt gcccttcagc gaggctttgg tacaaccatt
    26881 ttcaccaaac agaacttctt caacctgtgg cgtatccggc accataataa aaatgatgtc
    26941 cgatgcttcc gttaccaaac gcgcagtttc aacactaacg gcacccagtg acagtaattc
    27001 atcagcaacc ggaccaatgg ttgtgacatg cagttgatga ccggcacgcg ccagattaat
    27061 ggccatcggt gtacccataa tgcctaagcc aataaatccc agtttcataa ttaacctctt
    27121 ttaaatttcg cttttcctga attcagacaa cacgatcccc tgccaactta gcggcctaaa
    27181 aaagcattgt ctgaatagcg ttacgtttta acggtacgga tccatccagc gtaaaccggc
    27241 ttccgtggtg gtttggggtt tatattcaca cccaacccaa ccgttgtagt cagaattttc
    27301 aattaccgta aagagatatt cgtaattaat ttctccggtt cccggttcgc cgcgatgcgg
    27361 attatcggca atttgcaggt gaccaatttt atcggcccat tgagtcatag tgttggttaa
    27421 ttcaccttcc atccgctgca tatgataaat gtcatactga attttcaaat tgcagcaacc
    27481 aacatcatca atcaatttca gcgcctgccg agttccggtg agatggaaac caggaatatc
    27541 aaaatggttg ataggttcaa tcagtaataa aatatcttct ttcatcagca tattcgcggc
    27601 ataacgcagg ttttctacaa gcgttgcgtg aatctgttca ctgctgaaac cagccggcgt
    27661 tttaccgacc agacagttaa tttttttatt acccagcgca cgagcataac gaatcgctgc
    27721 tgctacgcca tcccgaaact cttcttcacg gccaggaata caggcaatac cgcgctcgcc
    27781 cgccgcccag tcacccgccg gtagattgtg cagcgtatgt tcgagtttat tactcgccag
    27841 cacctgtttt aattcttcaa tgtcgtagtc ataaggaaac ataaattcaa cgccgcgaaa
    27901 accacactgc gcagctttct caaaacgggc gagaaaatca tattcaccaa ataacatcga
    27961 taaattagca gagaaacgta acattattta tctcccttat tcatagtgca tgaagcaggt
    28021 ttcagtcggt gcgtccactg cgttatcggc gatatcttca aattccatga cgttatccag
    28081 ttcgctgccc atcgaaatat tggtcacacg ctcgagaata acttccacga ctaccggtac
    28141 ccgatattgc gccattaagg ctttcgcctg ttcaaaggct ggtgcaatat cttccggttt
    28201 gaagacccga atcgctttac aacctaaacc ttccgctact tttacgtggt cgacgccgta
    28261 accgttcact tcactggagt taatattttc gaacgcgagc tgtacgcagt aatccatatc
    28321 aaacgcgcgc tgcgactggc gaatcagacc cagataggcg ttattgacca gtacatggat
    28381 gtacggaatg ttgaactgtg cgccaacggc taattcttca atcaggaact ggaagtcgaa
    28441 gtcaccggaa atcgccacca cattgcgctc tggatcagcg gcacacacgc ccagcgccgc
    28501 cggaatggtc cagcccaacg ggcctgcctg cccacagttg atccagtggc ggtctttaaa
    28561 gacatgtagc atttgtgccg cagcgatttg tgacaggcca atggtggtga cataacaaac
    28621 atcgcgacca aaggctttgt tcatctcttc atacacgcgc tgcggtttca ccggcacatt
    28681 gtcgaagtgg gttttgcgca gcaaagtgcg tttgcgctgc tggcagtcgg cgacccattc
    28741 tttacgacac ggcagacgac ccgctttttg catctcctgc gccacttcaa ccagcagcgt
    28801 cagcgccgct ttagcatcag agacaatgcc cagatccgga cacagcacgc ggccaatttg
    28861 cgtcggctca atatcgatat gaacgatttt gcgcccttcg gtgtatttct ctaccgaacc
    28921 ggtatgacgg ttcgcaaaac ggttaccgat accaaacacc atgtcggacg ccagtaacgt
    28981 tgcgttaccg taacgatgcg cggtttgcag acccaccatc ccggccatca gttcatgatc
    29041 gtccgggata cagccccagc ccatcagcgt tgggatcacc ggaacgctgg tcagttcagc
    29101 aaactgttgt aacagtgcag ctgcgtcagc attgattacc ccgcccccgg caacaatcac
    29161 cggacgttcg gcctgaatta acatttctac cgctttttcg atctgcatac ggctggcagc
    29221 aggtttgtag accggcagcg gttcgtacat gtcaggatca aactcgattt ccgcaacctg
    29281 aacgtcgaac ggtaaatcca ccagtaccgg acccggacga ccagaacgca tcaggtgaaa
    29341 tgcctgttgc agcacgcgag gcaccagcgc cgcttcacga actgtaaccg ccattttgct
    29401 gaccggttta gcaattgctt caatatctac ggcctgaaaa tcttctttat gcagacgggc
    29461 gcgcggagcc tggccggtaa tacacagaat aggaatggaa tcagcagaag cagaatagag
    29521 cgcggtgatc atgtccgtgc ccgcaggacc ggaagtcccc agacatacgc cgatattccc
    29581 tgccgttgcg cgggtataac cttccgccat gtgcgaagca ccttccacat gacgcgccag
    29641 aatgtgacga ataccaccgt gcttacgcat cgctgagtag aacggattga ttgcagctcc
    29701 cggaacgccg aaggcggtgg tgataccttc tttctccagc acatacattg ccgcgtcaac
    29761 ggctctcatt tttgccattt tattcctacc tctattaatt ggaaaaattt tccaactttc
    29821 aaattttgtc aaaaaaaccc gctccgcaga gcgggatgcg acagacacta tggatgtgct
    29881 ttcagtccca acgccgtgct gatatcgcgg gcggtgtctc tgaccagctc accctgactg
    29941 acaaaacgat cttctgtcag tcttgatgaa ggcccggaga tggagatagc ggcaacaaca
    30001 ctaccgacat catcgtaaat tgctgaagct atacaattca ggccgacaac atgctcttct
    30061 ttatctacgg tatagcccag ttcacgcgct tgttccaggt ccttcagcaa ggtgggcata
    30121 tccacaagcg tcgttggcgt aaactgctgc aaaccggttt gcagaatgat gctcatcaac
    30181 tcttcttccg ccagcggata aagcagcgcc ttgcccgctc cggaagcatg cagcggcaga
    30241 cgactgccca gcggcgcaca catcctgacc atcgatttac actctaactg accaattaat
    30301 accgcttcat tgccgttacg gatcgcgaca ttgaccgttt cgccggaaag taacatcagg
    30361 cggcgcataa acggcccggc tacggagagg acatcgcggt tatggatgta cgccgcaccg
    30421 acgttaaaga cacctaatcc tatatgccac cagcctaact gactgtcctg atagacaaaa
    30481 tccgctgcct gtaaaacctt cagcaagcga aaggtcgtgg agagcggcaa atccagattg
    30541 agagaaatat cgctaaccga cgaacttccc ccactttttt ccagatattg cagaatcgca
    30601 attccccgct ctaacgcctg tgcgcccttc tgtgccacag gctccgcctg tcctggcctg
    30661 ccgcgccgtc taacttccgt catacctacg ttaactccga cctacaagtg agcataaaca
    30721 atgaacaatt agtttatctt atgcaagtcg gttgcaggcg tcacgcaaaa cagagttcct
    30781 gttcaggaat actttcaaca tcacagttgt cactgccgcc gcgatcgatg gtcagaaaat
    30841 cggtgacgcg ctgccaggcg aaaagtgggt gatgccagac gttacgatgg taattcaccc
    30901 cctgttcacc gttggtgata aacgcccgca gcgttgacag gtctggtttg tcgtcaccta
    30961 acgccacgac caccacaaac acttcacctt tcatcgggat aaaggcctga gtacccagcg
    31021 gatgacgttc gagttcgtga atggtcagcg gcagattcgc cggttgcgcg cggttaatgc
    31081 tgataagcgt acggtcttgc tcaagaatct caaccagcgc caaatcgtgg taacgctcca
    31141 ccaggccatt gttaatatgg aaaaaatccc gttgctgcgt ttcgattacg tcgccataag
    31201 cactaaaggc ttcctgactt aacggtaata cctgaagttt catctttttg tccttgtaac
    31261 ataattccgt tcaaccaccg ccgaccggtc agcttggttt caataacagg caatctaaaa
    31321 ccttaagaga acaacatcaa cacaaaagtt ggaatatttt tccaaaacta gatttagtcc
    31381 acaaattgaa cagggttatc atgtaaaaat actgataaat cagaggataa gaaaatcacg
    31441 caacttgcgg atttaaaagg ggatacctga agatgttcga tccagaaacc ttgcggactt
    31501 tcattgcggt tgctgaaaca ggaagttttt caaaagcggc agaacgattg tgtaaaacca
    31561 cggcgacgat cagttatcgc attaaacttc tggaagagaa taccggagtg gcgctgtttt
    31621 tccgcacgac tcgcagcgtg acgttgacag cggctggcga gcatctactt tctcaggcca
    31681 gagactggct gagctggctg gaaagtatgc ccagcgagct gcaacaggtg aatgatggcg
    31741 tggaacgcca ggtgaatatt gtcatcaaca acctgctcta caacccccag gccgtcgccc
    31801 agctgctggc gtggctgaat gaacgttacc cgtttaccca gtttcacatc tcccgacaaa
    31861 tctatatggg cgtctgggac tcgctattgt acgaaggttt ttcactggct atcggcgtca
    31921 cgggaaccga ggcgctggca aataccttta gtcttgatcc cttaggatcg gtgcaatggc
    31981 gctttgtcat ggcggcggat catccgctgg cgaacgttga agagccgcta acagaagcac
    32041 agttgcggcg ctttccggcg gtcaatattg aagacagcgc ccgcacacta accaaacgcg
    32101 tcgcctggcg attgccgggg caaaaagaga ttattgttcc cgatatggaa acgaaaatcg
    32161 ccgcccatct ggcgggcgtt ggcattggtt ttttgccaaa atcactttgc cagtcaatga
    32221 tcgataatca acaactggtc agccgggtaa tcccaacgat gcgccctcct tcaccattga
    32281 gtctggcatg gcgcaaattt ggtagcggca aagcggtaga agatattgtg accttgttta
    32341 cccagcgcag gccggaaatc agcggatttt tagaaatttt cggcaaccca cgcagttaaa
    32401 aatcaagttt ccttgcgcgt tggcgcgtaa aatagcgcgc aagctgattt gaggttcact
    32461 tttaactatg caagagagac acacggaaca ggactatcgt gccctgctga ttgctgatac
    32521 gcccattatt gatgttcgcg cccccatcga gtttgagcaa ggcgcaatgc ccgccgctat
    32581 caacctgccg ttaatgaata acgatgaacg tgccgccgtt ggcacctgct ataaacagca
    32641 aggctcagac gcggcgctgg cgctggggca taaactggtg gcgggtgaaa ttcgtcagca
    32701 gcgcatggac gcctggcggg cagcgtgcct gcaaaatccg caaggtattc tctgctgcgc
    32761 ccgaggcggt caacgttcac atattgtgca acgctggctg catgaagcgg ggattgatta
    32821 tccgctggtg gaaggcggtt ataaggcact gcgccagacg gcgattcagg cgactattga
    32881 actggcacaa aaaccgatag tgctgattgg cggttgtacc ggcagcggta aaacgctgtt
    32941 agtgcagcaa cagccaaacg gtgttgatct ggaagggctg gcgcgtcatc gcggttcggc
    33001 gtttggtcgc acgttacaac cacaacttag ccaggcaagt tttgaaaacc tgctggctgc
    33061 cgaaatgcta aaaaccgacg cccgtcagga gctgcacctg tgggtgctgg aagatgaaag
    33121 ccggatgatc ggttcgaatc acctgccgga atgtctgcgc gagcgaatga cccaggcggc
    33181 gattgcggtg gtagaagacc cgtttgagat ccgtcttgaa cgcctgaacg aagagtattt
    33241 cctgcgtatg catcatgatt ttacccatgc gtacggcgac gaacagggct ggcaggagta
    33301 ttgcgaatac ctgcatcacg gactttcggc gattaagcgt cggctggggc tacagcgcta
    33361 taacgaactg gctgcacgtc tggatgcggc gctggcaacg caactcacca ctggcagcac
    33421 cgacggtcat ctggcctggc tagtgccgtt acttaaagaa tattacgacc caatgtatcg
    33481 ctatcagctt gagaaaaaag cggaaaaagt tgtctttcgc ggtgagtggg cggaagtggc
    33541 ggaatgggtt aagacgcagt gatgagggac gtatttgtat tagtcgcgac ttgatctgac
    33601 acatggcctt gaaaggttga gagttaccgg ttttgatatg agtgtcgaat ccttatccaa
    33661 aacaagaggt aactctcatg cttcatactg ccaatcccgt catcaaacac aaagccggtt
    33721 tgctcaatct ggctgaagaa ctcagcaacg tatcaaaagc ctgtaaaatc atgggcgttt
    33781 cgcgtgaaac gttttatcgt taccgcgaac tggccgctga aggcggtgtg gatgtgcaga
    33841 ttaatcgtag tcgccgcgct cctaacctta agaaccgtac cgatgaggca actgaacagg
    33901 ctgttgttga ttacaccgtc gctttcccga cacatggtca gcaccgggcc agcaacgagc
    33961 tgcgtaaaca gggggttttt atctccgaca gtggtgtccg ttctgtctgg ttactccata
    34021 accttgagaa tctcaaaaga cgatactgaa caaattttat caggtgacgt tccgtaaaaa
    34081 gttgtatgga gttctcgatg cagtcggcgc gactatcagt acccccgctg aaccctggac
    34141 tatcgggccg ccgtactggg gcatgtcacc ctggcgtgcc gctggttttc cgctaatggt
    34201 ttatttcctc tttctttccc ggcgagaatt tattttaatc cggaaggatt atttattttc
    34261 agatagggac tcacattaaa cacgaaacat tttaattaat gcatggctga atatgtatta
    34321 cacaatgtca gatatcgtaa aagactagtg caattctatg taaactctct gactattcat
    34381 tttatttgtt gtttcagggt cggaactcac cctcaataaa caactgaata cgagtagata
    34441 ttcatcgtct gagctatatg gctttacaca atagccgaca tgtcttttct cagtcaggga
    34501 ttttactttt cctcactacg aagttgacga cttcgatatg ggatagactc ttaattcaag
    34561 caattactct gcacaagcat caggcaatcc agctccaata tttgttataa aataatgcgt
    34621 aacattaatt tcaccaacaa aaaaaaggtt tacgctatcc ccttgccctg gtattttacc
    34681 atatttacac cacacttttt ttgtatctgg atcattctct gatacgattt tcgccacttg
    34741 ttccctggcc ctcaccgtca atgttttctt aaacctcccc ccatcatcga ttgttaggga
    34801 aaaataagaa aaaaaataat catcttctac atttgtggat attgatatat ccaccgctac
    34861 atgcggaaaa aaatctggga gccatgtaaa aaccccatca ctttctggta ttgccatata
    34921 gtttatgtaa tgcttactta caagagattc atctttgtgt aaataaagat aagtagtcac
    34981 gcataaaaaa acaacgatta taatagcaaa aataaatatt accatctttg agatgttact
    35041 tgagatagcc agcatcttgt aaagccttta tcgttttttt atgctctgga ttaatataat
    35101 caatacatct atttgagcaa t
//
LOCUS       DAPOZO010000050        34063 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPOZO010000050 DAPOZO010000000
VERSION     DAPOZO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.050"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            41..1612
                     /gene="leuA"
                     /locus_tag="ROI21_002592"
     CDS             41..1612
                     /gene="leuA"
                     /locus_tag="ROI21_002592"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDX5111501.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            1612..2703
                     /gene="leuB"
                     /locus_tag="ROI21_002593"
     CDS             1612..2703
                     /gene="leuB"
                     /locus_tag="ROI21_002593"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDX5111502.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVAEGV"
     gene            2706..4106
                     /gene="leuC"
                     /locus_tag="ROI21_002594"
     CDS             2706..4106
                     /gene="leuC"
                     /locus_tag="ROI21_002594"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDX5111503.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLRAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVTIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4117..4722
                     /gene="leuD"
                     /locus_tag="ROI21_002595"
     CDS             4117..4722
                     /gene="leuD"
                     /locus_tag="ROI21_002595"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDX5111504.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVQANPGIYFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPEFMR"
     gene            complement(4830..4961)
                     /gene="sgrT"
                     /locus_tag="ROI21_002596"
     CDS             complement(4830..4961)
                     /gene="sgrT"
                     /locus_tag="ROI21_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDX5111505.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSGY"
     gene            5050..6708
                     /gene="sgrR"
                     /locus_tag="ROI21_002597"
     CDS             5050..6708
                     /gene="sgrR"
                     /locus_tag="ROI21_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDX5111506.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSCLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAA
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     SSNQLKIQAFDDFFGYRALIDEVNVWVLPEIADDPAGGLMLKGPQDEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHTRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(6938..7507)
                     /locus_tag="ROI21_002598"
     CDS             complement(6938..7507)
                     /locus_tag="ROI21_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HDX5111507.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTHSQIIRMVNLVKKTENMNIFE
                     ELWETLRNLFRSDKHSQTAARQILKDAFYFHNSDDYSKYFTGAVDGDARDKFTHWLIN
                     FNKLKEHAIDPENMAAKASLSSGDTLRVSFFIGDEAILTLDLQLKESTSTGCIDLSHA
                     YFNGVVICGIDRLEVDLSNAETNNSRWYD"
     regulatory      7748..7847
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7878..8861
                     /gene="thiB"
                     /locus_tag="ROI21_002599"
     CDS             7878..8861
                     /gene="thiB"
                     /locus_tag="ROI21_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDX5111508.1"
                     /translation="MLKKYLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKMVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYATANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            8837..10447
                     /gene="thiP"
                     /locus_tag="ROI21_002600"
     CDS             8837..10447
                     /gene="thiP"
                     /locus_tag="ROI21_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDX5111509.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRVRQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            10431..11129
                     /gene="thiQ"
                     /locus_tag="ROI21_002601"
     CDS             10431..11129
                     /gene="thiQ"
                     /locus_tag="ROI21_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDX5111510.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTSTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKASASALLGITG"
     gene            complement(11243..12007)
                     /locus_tag="ROI21_002602"
     CDS             complement(11243..12007)
                     /locus_tag="ROI21_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDX5111511.1"
                     /translation="MQALLEHFITQSTVYSLIAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAVGVVALVVLIRHPLMPMYIDILRKVVGG"
     gene            complement(12093..12971)
                     /gene="araC"
                     /locus_tag="ROI21_002603"
     CDS             complement(12093..12971)
                     /gene="araC"
                     /locus_tag="ROI21_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDX5111512.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13310..15010
                     /gene="araB"
                     /locus_tag="ROI21_002604"
     CDS             13310..15010
                     /gene="araB"
                     /locus_tag="ROI21_002604"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDX5111513.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTAGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15021..16523
                     /gene="araA"
                     /locus_tag="ROI21_002605"
     CDS             15021..16523
                     /gene="araA"
                     /locus_tag="ROI21_002605"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDX5111514.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKEDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            16692..17387
                     /gene="araD"
                     /locus_tag="ROI21_002606"
     CDS             16692..17387
                     /gene="araD"
                     /locus_tag="ROI21_002606"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDX5111515.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVLVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17462..19813
                     /gene="polB"
                     /locus_tag="ROI21_002607"
     CDS             17462..19813
                     /gene="polB"
                     /locus_tag="ROI21_002607"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDX5111516.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVI
                     IYEADVRPPERYLMERFITSPVWGEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            19977..22883
                     /gene="rapA"
                     /locus_tag="ROI21_002608"
     CDS             19977..22883
                     /gene="rapA"
                     /locus_tag="ROI21_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDX5111517.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            22895..23554
                     /gene="rluA"
                     /locus_tag="ROI21_002609"
     CDS             22895..23554
                     /gene="rluA"
                     /locus_tag="ROI21_002609"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDX5111518.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            23767..23945
                     /locus_tag="ROI21_002610"
                     /pseudo
     CDS             23767..23945
                     /locus_tag="ROI21_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(23962..24720)
                     /locus_tag="ROI21_002611"
     CDS             complement(23962..24720)
                     /locus_tag="ROI21_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP pyrophosphate phosphatase"
                     /protein_id="HDX5111519.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKMDVKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVDENEKEKFIHSLELFNELKTCA
                     KNPDEMVAKGKMRWVAEIYGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLGEAYL
                     PGVDMRDCYFGKKTIIDKKALYLEPGWNANLDGAILDGVILDGATVDGATRLYAEVII
                     INKITPKKIVTEGVATKQSTAEKLLPTQLLNDPE"
     gene            complement(25015..25830)
                     /gene="djlA"
                     /locus_tag="ROI21_002612"
     CDS             complement(25015..25830)
                     /gene="djlA"
                     /locus_tag="ROI21_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDX5111520.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26085..28439
                     /gene="lptD"
                     /locus_tag="ROI21_002613"
     CDS             26085..28439
                     /gene="lptD"
                     /locus_tag="ROI21_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDX5111521.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28492..29778
                     /gene="surA"
                     /locus_tag="ROI21_002614"
     CDS             28492..29778
                     /gene="surA"
                     /locus_tag="ROI21_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDX5111522.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29778..30767
                     /gene="pdxA"
                     /locus_tag="ROI21_002615"
     CDS             29778..30767
                     /gene="pdxA"
                     /locus_tag="ROI21_002615"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDX5111523.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVAQLAQREWPVELVVCADATLLT
                     DRAAMLGLTLTLRPYSPTSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRVQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            30764..31585
                     /gene="rsmA"
                     /locus_tag="ROI21_002616"
     CDS             30764..31585
                     /gene="rsmA"
                     /locus_tag="ROI21_002616"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDX5111524.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            31588..31965
                     /gene="apaG"
                     /locus_tag="ROI21_002617"
     CDS             31588..31965
                     /gene="apaG"
                     /locus_tag="ROI21_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDX5111525.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31972..32820
                     /gene="apaH"
                     /locus_tag="ROI21_002618"
     CDS             31972..32820
                     /gene="apaH"
                     /locus_tag="ROI21_002618"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDX5111526.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAVAS"
     gene            complement(32880..33131)
                     /locus_tag="ROI21_002619"
     CDS             complement(32880..33131)
                     /locus_tag="ROI21_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HDX5111527.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKF"
     gene            complement(33134..33367)
                     /locus_tag="ROI21_002620"
     CDS             complement(33134..33367)
                     /locus_tag="ROI21_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HDX5111528.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(33453..33932)
                     /gene="folA"
                     /locus_tag="ROI21_002621"
     CDS             complement(33453..33932)
                     /gene="folA"
                     /locus_tag="ROI21_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDX5111529.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
BASE COUNT         8091 a         9138 c         9039 g         7795 t
ORIGIN      
        1 cccgaagcga ggcgctcaaa aagagacaag gacccaaacc atgagccagc aagtcattat
       61 tttcgatacc acattgcgcg acggtgaaca ggcgttacag gcaagcttga gtgtgaaaga
      121 aaaactgcaa attgcgctgg cccttgagcg tatgggtgtt gacgtgatgg aagtcgggtt
      181 ccccgtctct tcgccgggtg attttgaatc agtgcaaacc atcgctcgcc aggttaaaaa
      241 cagccgcgta tgcgcgttag ctcgctgcgt ggaaaaggat atcgacgtgg cggctgaatc
      301 tctgaaagtc gccgaagcct tccggattca tacctttatt gccacttcgc caatgcacat
      361 tgccaccaag ctgcgtagca cgctggacga ggtgatcgaa cgcgctatct atatggtgaa
      421 acgcgcccgt aattacaccg atgatgttga attttcttgc gaagatgccg gacgcacccc
      481 aattgccgat ctggcgcgag tggtcgaagc ggcgattaat gccggtgcca ccaccatcaa
      541 catcccggac accgtgggct acaccatgcc atttgagttc gccggaatca tcagcgggct
      601 gtatgaacgc gtacccaaca tcgacaaagc cattatttcc gtacataccc acgacgattt
      661 gggcctggcg gtcggaaact cactggcggc ggtacatgcc ggtgcacgcc aggtggaagg
      721 tgcaatgaac gggatcggcg agcgtgccgg taactgttcc ctggaagaag tgatcatggc
      781 gatcaaagtt cgtaaggata ttctcaacgt ccacaccgcc attaatcacc aggaaatatg
      841 gcgcaccagc cagttagtta gccagatttg taatatgccg atcccggcaa acaaagccat
      901 tgttggcagc ggcgcattcg cacactcctc cggtatccac caggatggcg tgctgaaaaa
      961 ccgcgaaaac tacgaaatca tgacaccaga atctattggt ctgaaccaaa tccagctgaa
     1021 tctgacctct cgttcggggc gtgcggcggt gaaacatcgc atggatgaga tggggtataa
     1081 agaaagtgaa tataatttag acaatttgta cgatgctttc ctgaagctgg cggacaaaaa
     1141 aggtcaggtg tttgattacg atctggaggc gctggccttc atcggtaagc agcaagaaga
     1201 gccagagcat ttccgtctgg attacttcag cgtgcagtct ggctctaacg atattgccac
     1261 tgccgccgtc aaactggcct gtggcgaaga agtcaaagca gaagccgcca acggtaacgg
     1321 tccggtcgat gccgtctatc aggcaattaa ccgcatcact gactataacg tcgaactggt
     1381 gaaatacagc ctgaccgcca aaggccacgg taaagatgcg ctgggtcagg tggatatcgt
     1441 cgcaaattac aacggtcgcc gcttccacgg cgtcggcctg gccaccgata ttgtcgagtc
     1501 ctccgccaaa gccatggtgc acgtactgaa caatatctgg cgtgccgcag aagtcgaaaa
     1561 agagttgcaa cgcaaagctc aacacaacga aaacaacaag gaaaccgtgt gatgtcgaag
     1621 aattaccata ttgccgtatt gccgggggac ggtattggtc cggaagtgat gacccaggcg
     1681 ctgaaagtgc tggatgccgt gcgcaaccgc tttgcgatgc gtatcaccac cagccattac
     1741 gatgtaggcg gcgcagccat tgataaccac gggcagccgc tgccgcctgc gacggttgaa
     1801 ggttgtgagc aagccgatgc cgtgctgttt ggctcggtag gtggtccgaa gtgggaacat
     1861 ttgccgccag accagcaacc agaacgcggc gcactgttgc ctttgcgtaa acacttcaaa
     1921 ttattcagca atctgcgtcc ggcaaaactg tatcaggggc tggaagcatt ctgtccgctg
     1981 cgtgctgaca ttgccgctaa cggcttcgac atcctgtgtg tgcgcgaact gaccggcggc
     2041 atctatttcg gtcagccaaa aggccgcgaa ggtagcggac aatatgaaaa agcgtttgat
     2101 accgaggtgt atcaccgttt tgagatcgaa cgtatcgccc gcatcgcgtt tgaatctgct
     2161 cgtaaacgtc gccataaagt gacgtctatc gacaaagcca acgtgctgca atcctctatt
     2221 ttatggcggg agatcgttaa cgagatcgcc acggaatacc cggatgtcga actggcgcat
     2281 atgtacatcg acaacgccac catgcagctg attaaagatc catcacagtt tgacgtcctg
     2341 ctgtgctcca acctgtttgg cgacattctg tctgacgagt gcgcaatgat cactggctca
     2401 atggggatgt tgccttccgc cagcctgaac gaacaaggtt ttggtctgta tgaaccggca
     2461 ggcggctccg caccagatat cgcaggcaaa aacatcgcca acccgattgc acaaatcctg
     2521 tcgctggcac tgctgctgcg ttacagcctg gatgccgatg atgcggcttc cgccattgaa
     2581 cgcgccatta accgcgcatt agaagaaggc attcgcaccg gagatttagc ccgtggcgct
     2641 gccgccgtta gtaccaatga aatgggcgat atcattgccc gctatgtggc agaaggggtg
     2701 taatcatggc taagacgtta tacgaaaaat tgttcgacgc tcacgtagtg tacgaagccg
     2761 aaaacgaaac cccgctgtta tatatcgacc gccacctggt gcatgaagtg acctcaccgc
     2821 aggcgttcga tggtctgcgc gcccacggtc gcccggtacg tcagccgggg aaaaccttcg
     2881 ccaccatgga tcacaacgtc tctactcaga ccaaagacat taatgcctgc ggtgaaatgg
     2941 cgcgcatcca gatgcaggag ctgatcaaaa actgcaaaga gtttggcgtc gagctgtatg
     3001 acctgaatca cccgtatcag gggatcgtcc atgtaatggg gccggaacag ggcgtcacat
     3061 tgccggggat gactattgtc tgcggcgact cgcataccgc cacccacggc gcgtttggcg
     3121 cactggcctt tggtatcggc acttccgaag ttgaacacgt actggcaacg caaaccctga
     3181 aacagggtcg tgcgaagacc atgaaaattg aagtccaggg caaagctgcg ccaggcatta
     3241 ccgcgaaaga tatcgtgctg gcaattatcg gtaaaactgg cagcgcaggc ggcaccgggc
     3301 atgtggtgga gttttgcggc gaagcaatcc gtgatttaag catggaaggt cgtatgaccc
     3361 tgtgcaatat ggcaatcgaa atgggcgcga aagccggtct ggttgcaccg gacgaaacca
     3421 cctttaacta tgtcaaaggc cgtctgcatg cgccgaaagg caaagatttc gacgacgccg
     3481 ttgcctactg gaaaactctg caaaccgacg aaggcgcaac tttcgatacc gttgtgactt
     3541 tgcgggcaga agagatttca ccgcaggtca cctggggtac caatccaggt caggtgattt
     3601 ccgtgaacga caatattccc gatccggctt cgtttgccga tccggttgaa cgcgcgtcgg
     3661 cagaaaaagc gttggcctat atggggctga aaccgggtat tccgctcacc gaagtgacta
     3721 tcgataaagt gtttatcggt tcctgcacca actcgcgcat tgaagattta cgcgcggcag
     3781 cggaaatcgc caaaggccgg aaagtcgcgc caggtgtaca agcgctggtg gttcccggct
     3841 ccggtccggt aaaagcgcag gcggaagcgg aaggtttgga caaaatcttt atcgaagctg
     3901 gttttgaatg gcgcttgcct ggctgctcaa tgtgtctggc gatgaacaac gatcgcctga
     3961 atccgggtga gcgttgtgcc tccaccagca accgtaactt tgaaggccgc caggggcgtg
     4021 gagggcgcac ccatctggtc agcccggcaa tggctgccgc tgctgcggtg accggacatt
     4081 tcgccgacat tcgcaacatt aaataaggag cacaccatgg cagagaaatt tatcaaacac
     4141 acaggcctgg tggttccgct ggatgccgcc aatgtcgata ccgatgcaat catcccgaaa
     4201 cagtttttgc agaaagtgac ccgtactggt tttggcgcgc atctgtttaa cgactggcgt
     4261 tttctggatg aaaaaggcca acagccaaac ccggacttcg tgctgaactt cccgcagtat
     4321 cagggcgctt ccattttgct ggcacgagaa aacttcggct gcggttcctc gcgtgagcac
     4381 gcgccgtggg cattgaccga ttacggtttt aaagtggtga ttgcaccgag ttttgctgac
     4441 atcttctacg gcaacagctt taacaaccag ctgctgccgg tgaaattaag cgacgcagaa
     4501 gtggacgaac tgtttgcgct ggtgcaggcc aatccgggga tttatttcga cgtggacctg
     4561 gaagcgcaag aggtgaaagc gggagagaaa acttatcgat ttaccattga tgccttccgc
     4621 cgccactgca tgatgaatgg tctggacagc attggcctga ccctgcagca cgacgacgct
     4681 attgcgtctt atgaagagaa gcaaccggaa tttatgcgtt agtttgtaaa ccgtccggca
     4741 ctcaatcgcc aaaaagaaaa aagccagcag ataatcactg ctggcgtggc gattaacacc
     4801 aatactcagt cacacatgat gtccgcaaat cagtagccag aatgactccc ctcccactgc
     4861 atcagttctt caagcatggt taatcgttgt gggacgctta accaacgcaa ccagcacaac
     4921 ttcgctgtcg cggtaaaata gtgctgataa aactgacgca tggggcacct ccttgcttca
     4981 tcgttaggaa ttattgactt aatataggga aaataaaatt gctgtctttt gcgcaggagt
     5041 tcccctttta tgccatctgc tcgtctgcaa caacagttca tccgcctgtg gcaatgctgc
     5101 gagggtaaat cgcaggacac gacgctcaac gaactggcag cgttattgag ctgctcgcgt
     5161 cgtcatatgc gcaccctgct caacaccatg caggatcgcg gctggctgac gtgggaagcg
     5221 gaagtcgggc gcggcaaacg ctcgtgtctg acattcctct ataccgggct ggcgcttcag
     5281 caacagcgag cagaggacct gctggagcag gatcgtatcg atcaactggt gcaactggtt
     5341 ggcgacaaag cggctgtgcg gcaaatgttg gtttctcatc tgggacgcag cttccgccag
     5401 gggcggcaca tcctgcgtgt gctctactat cgtccgttgc gtaatctgct gcccggcagt
     5461 gcattgcgcc gttccgaaac ccatatcgcc cggcaaatct tcagttcgct aacgcgcata
     5521 aatgaggaaa atggggaact ggaagcggac atcgcccacc actggcagca aatttcaccg
     5581 cttcactggc gtttcttttt gcgtccagga gtccattttc atcatggtcg tgaactggaa
     5641 atggacgacg tgatcgcctc tttaaaacga atcaatacgc tgccgctcta ttcgcatatt
     5701 gctgacattg tgtcgccgac gccctggacg ctggatatcc atctcacgca accggaccgc
     5761 tggttaccgt tactgctggg gcaagttccg gcgatgatcc tgccgcgcga atgggaaacc
     5821 ctcagcaact ttgccagcca tcccatcgga acgggtcctt atgcggtgat ccgcaacagc
     5881 agcaatcaac tgaaaattca ggcattcgac gacttctttg gttatcgggc attaatcgac
     5941 gaagttaacg tctgggtctt gccggaaatt gctgatgacc ccgcgggagg cctgatgctg
     6001 aaaggaccgc aagacgagga aaaagagatt gaaagccgcc ttgaagaagg ttgttactat
     6061 ttactgttcg acagccgtac ccatcgcggg gcgaatcagc aagtcagaga ctggataagc
     6121 tatgtgcttt cgccgaccaa tctggtttat ttcgccgagg aacagtacca gcagctgtgg
     6181 tttccggcct atgggctgct tccccgttgg caccataccc gcaccataaa gagcgaaaaa
     6241 ccggctggtc tggaaagcct caccctgacc ttttatcagg atcacagtga gcatagggtg
     6301 attgccggga tcatgcagca gattctggca agtcaccagg tcacgctgga aatcaaagag
     6361 atcagctacg atcagtggca tgaaggagag atcgagagcg atatctggct taacagcgcc
     6421 aactttacgc tgccgctgga tttttcgcta ttcgcgcacc tgtgcgaggt gccgctgctc
     6481 caacactgta ttccgatcga ctggcaagcc gacgccgccc gctggcgcaa tggcgaaatg
     6541 aacctggcga actggtgcca gcaactggtc gccagcaaag caatggtgcc gcttatccac
     6601 cactggctga ttattcaggg acaacgcagt atgcgcggcc tgcgcatgaa caccctcggc
     6661 tggttcgatt ttaaatcagc gtggtttgcg ccgccggatc cagagtagct tgcgcctttt
     6721 ggcggtcatg agcgcatttt ttatattcag acggaatgaa taaaatacat tacttttata
     6781 ttttctctat tttatgcatc gtctgattag cgtcgcaaat aagattaaag aaggggcatt
     6841 ccagcccctc tttcatattc tgagttaatc aataacggcg cagcctagcc gtgttctggc
     6901 cggaaaggtc agctttttca ggttaacctc gcgcaggtca gtcataccaa cggctattgt
     6961 tcgtttcagc attgctcagg tcaacctcga gacggtcaat accacaaatg acaaccccgt
     7021 taaagtaagc atgactcaga tcaatgcacc cggtactggt gctctctttc agctgcaaat
     7081 ccagagtaag tatcgcctca tctccaataa aaaaactgac tcttaacgta tctcctgacg
     7141 agagtgaggc cttcgcggcc atattttccg gatctattgc atgttctttg agtttattaa
     7201 aatttatcag ccagtgggta aacttatcac gcgcatcccc atcgacagcc ccggtaaagt
     7261 attttgagta gtcatcacta ttatggaaat aaaatgcatc cttcagaatt tgccttgccg
     7321 cagtctggga atgtttatct gaccggaaga gattgcgtaa cgtctcccat agctcttcaa
     7381 agatattcat attctcggtc tttttgacca ggttaaccat cctgataatc tgagaatgag
     7441 taaggcctgg atctttagtg gtattacttg cagggatact aatctgttct gggtttctta
     7501 ctttcatgct gatagctcca ctctgagtta aaaactcaga ataagcgtcc tgaataagaa
     7561 tactgtcaaa aatcgctcat tcacgcaata agccgttgca ttaccgattt tgactgtgct
     7621 aagggattct ttcggttcaa agcttcccag ctatgcaaat acgtccttga tagctgaacg
     7681 aatattggct tttttaatac accttctcta tggattgctg gttgataaca aaatcactac
     7741 actaacgccg ttctcaacgg ggtgccacgc agacgcgtgc gctgagaaaa tacccgtcga
     7801 acctgatccg gataacgccg gcgaagggat ttgaggctcc ttctcaagtc ctttgccact
     7861 cttttttgag gtgcaaagtg ttaaaaaaat atctgcccct gctgttgctg tgcacagcgc
     7921 ccgttttcgc taaacccgtt ctgaccgtct acacctacga ctccttcgcc gccgactggg
     7981 gtccaggtcc ggtggttaaa aaagcctttg aagccgactg taattgcgaa ctgaaaatgg
     8041 tggcgctgga agatggcgtt tcgcttctca accgtctgcg gatggaaggc aaaaacagca
     8101 aagccgatgt ggtgctgggg ctggataaca atctgttaga cgcagccagt aaaaccggac
     8161 tatttgccaa aagcggcgtg gcagcggatg ccgttaacgt tcccggcggc tggaataatg
     8221 acactttcgt accgtttgat tacggctatt tcgccttcgt ctatgacaag aacaaactga
     8281 aaaatccgcc gcaaagcctg aaagagctgg ttgaaagcga tcaaaactgg cgggtgattt
     8341 atcaggatcc gcgcaccagc acgccgggac tgggtctgct gttgtggatg caaaaagtct
     8401 atggcgatga cgccccacaa gcctggcaga aactggcgaa gaaaacggtc acggtcacca
     8461 aaggctggag cgaagcctac ggcctgtttt taaaaggtga aagcgatctg gtcctgagtt
     8521 acaccacctc tccggcttat cacattctcg aagagaagaa agataactac gccaccgcga
     8581 acttcagcga aggtcactat ttgcaagtgg aagtcgccgc ccgcaccgct gccagcaagc
     8641 agccagagct ggcgcaaaaa ttcctccagt ttatggtttc tccggctttc cagaatgcga
     8701 tcccaaccgg caactggatg tatccggtgg caaatgtcac gctgcctgcc gggtttgaac
     8761 aattgaccaa accggcaacc acgctggagt tcacgccagc cgaagtggcg gcacaacgtc
     8821 aggcatggat tagcgaatgg caacgcgccg tcagccgtta attcccggct ggttaattcc
     8881 aggtgtaagc gccgctacgc ttgtggtggc ggttgcgctg gcggcgtttc tcgccctgtg
     8941 gtggaacgcg ccgcagggtg actgggtggc aatctggcag gacagctacc tgtggcatgt
     9001 ggtgcgcttc tccttctggc aggcgtttct ctcggcgcta ctctctgtcg tacccgcgat
     9061 attcctcgcc cgcgcgctct atcgcaggcg ctttccgggt cggctggcgc tgttgcgtct
     9121 gtgcgcaatg accttgatcc tcccggtgct ggttgctgtt ttcggcattc ttagcgtcta
     9181 tggtcgccag ggctggctgg catcgctctg ccaatcgctc ggtctggagt ggaccttttc
     9241 gccctacggc ctgcaaggta ttttgctggc gcacgtattt tttaatctgc cgatggcgag
     9301 ccgcttatta ctccaggcac tggaaaacat tcccggcgaa caacgtcagc ttgccgccca
     9361 gcttgggatg cgcggctggc attttttccg cttcgtcgaa tggccgtggt tacggcgaca
     9421 aatcccgcca gttgctgcgc ttatctttat gctctgtttc gccagcttcg ccaccgtgct
     9481 gtcgctgggc ggcggtccgc aggcgaccac tatcgagctg gctatttatc aggcgctgag
     9541 ttacgactac gatcctgccc gcgcggcgat gctggcgttg atccagatgg tgtgttgcct
     9601 cgggctagtg ctgttgagtc agcgattgag taaggccatt gcgcccggca ctactctgct
     9661 gcaaggctgg cgcgacccgg acgatcgtct gcatagccgc atttgcgaca cggtgttaat
     9721 tgtgctggcg ctgctgctgt tgctaccacc gttactggcg gtgatcgtcg atggggtaaa
     9781 tcgccagttg ccggaagtgc tggcacaacc ggtgctgtgg caggcgctgt ggacctcgtt
     9841 gcgtattgcg ctggcggcag gcgtattatg cgtagtgctg accatgatgc tgctatggag
     9901 cagtcgcgaa ctgcgggtgc ggcaaaaaat gctggcgggt caggcgctgg agatgagcgg
     9961 catgttgatc ctcgccatgc cggggattgt gctggctacg ggcttctttt tactgctcaa
    10021 caacactatc ggcctgccgc aatctgctga cggcattgtg attttcacca atgcgttaat
    10081 ggcgatccct tatgcgctga aggtgctgga aaacccgatg cgcgatatca ccgcgcgtta
    10141 cagtatgttg tgtcagtcgc tgggcattga aggctggtca cgcttaaaag tggtggagct
    10201 gcgcgccctg aaacgcccac ttgcgcaggc actggccttt gcttgtgtgc tatcgattgg
    10261 tgattttggc gtggtggcgt tgttcggtaa cgatgatttc cgcaccctgc cgttttatct
    10321 ctaccagcaa attggctcct atcgcagcca ggacggcgcg gtcaccgcgt taattctgct
    10381 gctactctgt tttctgctgt ttaccgtgat tgaaaaacta ccggggcgaa atgttaaaac
    10441 tgactgatat cacctggctt taccaccatt tgccgatgcg ttttagctta acggtggaac
    10501 ggggcgagca ggtggcgatc ctcgggccaa gcggcgcggg taaaagtacc ctgctgaatt
    10561 tgatcgccgg ttttctgacg ccagccagcg gttcgctgac tatcgacggc gttgatcaca
    10621 cctctacgcc gccgtcacgc cgtccggtgt cgatgctgtt tcaggagaac aacctgttca
    10681 gtcacctgac ggtcgcgcag aacatcgggc tggggcttaa tccaggattg aagctgaacg
    10741 cggcacaaca ggagaaaatg cacgctatcg cccgccagat ggggattgat aatttaatgg
    10801 cgcgattacc gggcgagctt tctggcggtc aacgacagcg cgtggcgtta gcgcgttgtc
    10861 tggtgcgcga acagccgatt ttattgctcg atgaaccgtt ctctgcgctc gatccggcgt
    10921 tacgtcaaga gatgttgacg ctggtgagca cgagctgcca gcagcaaaaa atgacgctat
    10981 tgatggtgtc gcacagtgtg gaagatgcgg cgcggatcgc cacacgctcg gtggtggtcg
    11041 ccgacgggcg catcgcctgg cagggtatga ccgatgagtt gttgagcggt aaggcgagtg
    11101 cttcggcact attggggatt acgggttagt atgcactgtc ggatgcggcg tgaacgcctt
    11161 atccgaccta cggacagtgc gttttgtagg cctgataaga cgcggcaagc gtcgcatcag
    11221 gcaacccgca caaggctatc ccctaacccc caaccacttt acgcaaaata tcgatataca
    11281 tcggcatcaa cgggtggcga attaacacca ccagcgccac tacaccgacg gcagaaatca
    11341 acggcgtcag ccacaacaaa cgaccgcgcg acaaataatg acttaaacga tcggttgctt
    11401 taccgctgcg ccataaccgc cagcacagcc agccaccaac ccacaaaaat accgccgtcg
    11461 ccagcagcaa ccatttaaac tcgccgctct gcattccggc tggaatatcg atcgccgcac
    11521 ccgccagaat ccccggcagg aagtaaaacg gtggccacag caggcagccg ataatattcg
    11581 gcgtaataaa ttttgccacc gggagatcca gcattcccgc caccattggc accagcggac
    11641 gcgtcgggcc aacaaaacga ccgaccagaa tggtgaacat gctgtgttga tgcaacgcat
    11701 gttcagtttt atcaagtagt gctttgtttt tcttcagaaa tgaccagcga tgcaatggct
    11761 ttttaaaacg ccagcccagc cagaaggaga tccagtcgcc cagcaggcaa ccgacgatcc
    11821 ctgccagcca ggcgtgccag aaacttaact cgccgctgcc aatcagcgcc cccagccccg
    11881 ccatcagcac cgtaccgggt agaatcaaac cgaccagcgc cagcgactcc agaaaggcca
    11941 ccaacaccac cgctatcaat gaatacacgg tggattgggt aataaagtgt tccagcaatg
    12001 cttgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc
    12061 gtcaagtcgt caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc
    12121 actttttctt cacaaccggc acggaactcg ctcggactgg ccccggtgca ttttttaaat
    12181 actcgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt agcaataggc
    12241 atccgggtgg tgctcaaaag cagcttcgcc tgactaatgc gttggtcctc gcgccagctt
    12301 aagacgctaa ttcctaactg ctggcggaac aaatgcgaca gacgcgacgg cgacaggcaa
    12361 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
    12421 tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc
    12481 atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct
    12541 tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct
    12601 tcatccgggc gaaagaaacc cgtattggca aatattgacg gccagttaag ccattcatgc
    12661 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga
    12721 cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcagaca
    12781 aattctcgtc cctgattttt caccaccccc tggccgcgta tggtgagatt gagaatataa
    12841 cccttcattc ccagtggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc
    12901 gttaaacccg ccaccagatg ggcgttaaac gagtatcccg gcagcagggg atcattttgc
    12961 gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc
    13021 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaatcca accggtaacc
    13081 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
    13141 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
    13201 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttttcg
    13261 caactctcta ctgtttctcc atacccgttt ttttggatgg agtgaaacga tggcgattgc
    13321 aattggcctc gattttggca gtgattctgt gcgagctttg gcggtggact gcgccaccgg
    13381 tgaagagatc gccaccagcg tagagtggta tccccgttgg cagaaagggc aattttgtga
    13441 tgccccaaat aaccagttcc gtcatcatcc gcgtgactac attgagtcaa tggaagcggc
    13501 gctgaaaacc gtgcttgcag agcttagcgc cgagcagcgc gcagctgtgg tcgggattgg
    13561 cgttgacaca accggctcga cgcccgcacc gattgacgcg gacggtaatg tcctggcgct
    13621 gcgcccggag tttgccgaaa acccgaacgc gatgttcgta ttgtggaaag accacaccgc
    13681 ggttgaagaa gcggaagaga taacccgctt atgtcacacg gcgggcaacg ttgactactc
    13741 ccgctatatt ggcggtattt attccagcga atggttctgg gcaaaaatcc tgcatgtgac
    13801 tcgccaggac agcgccgtgg cgcaatctgc cgcatcgtgg attgagctgt gcgactgggt
    13861 gccagctctg ctttccggta ccacccgccc gcaggatatt cgtcgcggac gttgcagcgc
    13921 cgggcataag tcgctatggc acgaaagctg gggcggcctg ccgccagcca gtttctttga
    13981 tgagctggat ccaatcctca atcgccattt accttccccg ctgttcactg acacctggac
    14041 tgccgatatt ccggtgggca ctttatgtcc ggaatgggcg cagcgtctcg gcctgcctga
    14101 aagcgtggtg atttccggag gcgcgtttga ctgccatatg ggcgcagttg gcgcaggcgc
    14161 acagcctaac gcactggtaa aagttattgg tacttccacc tgcgacattc tgattgccga
    14221 taaacagagc gttggcgagc gggcagtgaa aggcatttgc ggtcaggttg atggcagcgt
    14281 ggtgcctgga tttatcggtc tggaagcagg tcagtcggcg tttggtgata tctacgcctg
    14341 gttcggtcgc gtactcggct ggccgctgga acagcttgcc gcccagcatc cggaactgaa
    14401 agagcaaatc aacgccagcc agaaacaact gcttccggcg ctgaccgaag catgggccaa
    14461 aaatccgtct ctggatcacc tgccggtggt gctcgactgg tttaacggcc gccgcacacc
    14521 gaacgctaac caacgcctga aaggggtgat taccgatctg aacctcgcta ccgacgctcc
    14581 gctgctgttc ggcggtttga ttgctgccac cgcctttggc gcacgcgcaa tcatggagtg
    14641 ctttaccgat caggggatcg ccgttaataa cgtgatggcg ctgggcggca tcgcgcggaa
    14701 aaaccaggtc attatgcagg cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc
    14761 ctctgaccag tgctgtgcgc tcggtgcggc gattttcgct gccgtcgccg cgaaagtgca
    14821 cgcagacatc ccatcagccc agcaaaaaat ggccagtgca gtagagaaaa ccctgcaacc
    14881 gcgcagcgaa caggcacaac gctttgaaca gctttatcgc cgctatcagc aatgggcgat
    14941 gagcgccgaa caacactatc ttccaacttc cgccccggca caggctgccc aggccgttcc
    15001 gactctataa ggacacgaca atgacgattt ttgataatta tgaagtgtgg tttgtaattg
    15061 gcagccagca tctgtatggc ccggaaaccc tgcgtcaggt cacccaacat gccgagcacg
    15121 ttgttaatgc gctgaatacg gaagcgaagc tgccctgcaa actggtgttg aaaccgctgg
    15181 gcaccacgcc ggatgaaatc accgctattt gccgcgacgc gaattacgac gatcgttgcg
    15241 ccggcctggt ggtgtggctg cacaccttct ccccggccaa aatgtggatc aacggcctga
    15301 ccatgctcaa caaaccgttg ctgcaattcc acacccagtt taacgcggcg ctgccgtggg
    15361 atagcatcga tatggacttt atgaacctga accagactgc acatggcggt cgtgagttcg
    15421 gcttcatcgg cgcgcgtatg cgtcagcaac atgccgtcgt taccggtcac tggcaggata
    15481 aacaagccca tgagcgtatc ggctcctgga tgcgtcaggc ggtctctaaa caggataccc
    15541 gtcatctgaa agtctgccgt tttggcgata acatgcgtga agtggcggtc accgatggtg
    15601 ataaagttgc cgcacagatc aagttcggtt tctccgtcaa tacctgggcg gttggcgatc
    15661 tggtgcaggt agtgaactcc atcagcgacg gcgatgttaa cgcgctggta gatgagtacg
    15721 aaagctgcta caccatgacg cctgcgacac aaatccacgg cgaaaaacgc cagaacgtgc
    15781 tggaagcggc gcgtattgag ctggggatga agcgtttcct ggaacaaggt ggcttccacg
    15841 cgttcaccac cacctttgaa gatttgcacg gtctgaaaca gcttcctggt ctggccgtac
    15901 agcgtctgat gcagcagggt tacggctttg cgggcgaagg cgactggaaa actgccgccc
    15961 tgcttcgcat catgaaggtg atgtcaaccg gtctgcaggg cggcacctcc tttatggagg
    16021 actacaccta tcacttcgag aaaggcaatg acctggtgct cggctcccac atgctggaag
    16081 tctgcccgtc gatcgccgca gaagagaaac cgatcctcga cgttcagcac ctcggtattg
    16141 gcggtaaaga agatcctgcc cgcctgatct tcaacactca aaccggtccg gccattgtcg
    16201 ccagtctgat tgatctcggc gatcgttacc gtctgctggt taactgcatc gacacggtga
    16261 aaacaccgca ctccctgccg aaactgccgg tggcgaatgc gctgtggaaa gcgcaaccgg
    16321 atctgccaac tgcttccgaa gcgtggatcc tcgctggtgg cgcgcaccat accgttttca
    16381 gccatgcgct gaacctcaac gatatgcgcc agttcgccga gatgcacgac attgaaatca
    16441 cggtgattga taacgacacc cgcctgccag cgtttaaaga cgcgctgcgc tggaacgaag
    16501 tgtattacgg gtttcgtcgc taagtagccg catcaggtgt gtaacgcctg atgcgacgct
    16561 gacgcgtctt atcaggccta cacgctgtga atttgtaggc cggataagca aagcgcatcc
    16621 ggcattgttt tccccctcgc ccctttgggg agagggccgg ggtgagggga aacgacctct
    16681 caggagtcaa catgttagaa gatctcaaac gccaggtatt agaagccaac ctggcgctgc
    16741 caaaacataa cctggtcacg ctcacatggg gcaacgtcag cgccgttgat cgcgagcgcg
    16801 gcgtcttggt gatcaaacct tccggcgtcg attacaacgt catgaccgct gacgatatgg
    16861 tcgtggtcag catcgaaacc ggtgaagtgg ttgaaggaac gaaaaagccc tcctccgaca
    16921 cgccaactca ccggctgctc tatcaggcat tcccgtccat tggcggcatt gtgcacacac
    16981 actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc gattccagca accggtacca
    17041 cccacgccga ctatttctac ggcaccattc cctgcacccg caaaatgacc gacgcagaaa
    17101 tcaacggtga atatgagtgg gaaaccggta acgtcatcgt agaaaccttc gaaaaacagg
    17161 gaatcgacgc agcacaaatg cccggcgtcc tggtccattc tcacggccca tttgcatggg
    17221 gcaaaaatgc cgaagatgcg gtgcacaacg ccatcgtgct ggaagaagtc gcttatatgg
    17281 ggattttctg ccgccagctt gctccgcagt taccggatat gcagcaaacg ctgctggata
    17341 aacactatct gcgtaagcat ggtgcgaagg catattacgg gcagtaatga ctgtataaaa
    17401 ccacagccaa tcaaacgaaa ccaggctata atcaagcctg gttttttatt ggattttcag
    17461 cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc aagggacaga
    17521 agtctccttc tggctggcga cggacaacgg gccgttgcag gttacgcttg cgccgcaaga
    17581 gtccgtggcg tttattcccg ccgatcaggt tccccgcgcc cagcatattt tgcagggtga
    17641 gcaaggcttt cgcctgacgc cgctggcgtt aaaggatttt catcgccagc cggtgtatgg
    17701 cctttactgt cgcgcccatc gccagttgat gaattacgaa aagcgcctgc gtgaaggtgg
    17761 cgttatcatc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg agcggtttat
    17821 cacctcaccg gtgtggggcg agggtgatat acgcaatggc gctatcgtta atgcccgtct
    17881 gaaaccgcat cccgactacc gtccgccgct caagtgggtt tctattgata ttgaaaccac
    17941 ccgccacggt gagctgtact gcatcggcct ggaaggttgc gggcagcgca tcgtttatat
    18001 gctggggcca gagaatggcg acgcctccgc gctcgatttc gaactggagt acgtcgccag
    18061 ccgcccgcag ctactggaaa aactcaacgc ctggtttgcc aactacgatc ctgatgtgat
    18121 catcggctgg aacgtggtgc aattcgatct acgaatgctg caaaaacatg ccgagcgtta
    18181 tcgtattccg ctgcggctgg ggcgcgataa cagcgagctg gagtggcgcg agcacggctt
    18241 taaaaacggc gtcttttttg cccaggccaa agggcggcta attatcgacg gtatcgaggc
    18301 gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaaccgtcg ctcaggagtt
    18361 attaggcgaa ggaaaatcta tcgataaccc gtgggatcgg atggacgaaa ttgaccgccg
    18421 tttcgccgaa gataaacctg cgctggcaac ttataacctg aaagattgcg agctggtgac
    18481 gcagatcttc cacaaaactg aaatcatgcc atttttactc gaacgagcga cggtgaacgg
    18541 cctgccggtg gatcggcacg gcggttcggt ggcagcgttt ggtcatctct attttccgcg
    18601 aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccac acgccagccc
    18661 tggcggctat gtgatggatt cgcggccagg gctttatgat tcggtactgg tgctggacta
    18721 caaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg ggctggtgga
    18781 aggcatggcg cagcctgatc cagagcacag caccgaaggt tttctcgacg cctggttctc
    18841 gcgggagaaa cattgcctgc cggagattgt gactaacatc tggcacgggc gcgatgaagc
    18901 caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga atgcctttta
    18961 tggcgtgctt ggcaccaccg cctgccgctt cttcgatccg cgcctggcgt cgtcgatcac
    19021 catgcgtggt catcagatca tgcggcaaac caaagcattg attgaagcgc agggctacga
    19081 cgtgatctac ggcgataccg actcaacgtt tgtctggttg aaaggcgcac attcggaaga
    19141 agaagcggcg aaaattggtc gtgcactggt gcagcacgtt aacgcctggt gggcagaaac
    19201 gctgcaaaaa caacggctga ccagcgcatt agaactggag tatgaaaccc atttctgccg
    19261 ttttctgatg ccaaccattc gcggagccga taccggtagt aaaaagcgtt atgccgggct
    19321 gattcaggag ggcgataaac agcggatggt gtttaaaggg ctggaaaccg tgcgcaccga
    19381 ctggacgccg ctggcccagc agtttcagca ggagctgtac ctgcgtatct tccgcaacga
    19441 gccatatcag gagtacgtcc gcgaaaccat cgacaaactg atggcgggtg aactggatgc
    19501 gcggctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc gtaatgttcc
    19561 gcctcatgta cgcgctgcac gccttgccga tgaagaaaac caaaagcgcg gtcgcccctt
    19621 gcaatatcag aaccgcggca ccattaagta cgtatggacc accaacggcc cggagccgct
    19681 ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc tacaacccgt
    19741 ggcggaggga atactccctt ttattgagga taattttgct acacttatga ccgggcaact
    19801 tgggctattt tgagcaaaaa aagagttcgc cagataccat tttgatgcgt gacgaatgct
    19861 ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac cactacctca
    19921 taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga acacatatgc
    19981 cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga cttggaaccg
    20041 ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt gaaaaccgtc
    20101 tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt gataccatta
    20161 ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat ggcttgctga
    20221 cttatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt gaagttttcc
    20281 ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg cagattgacc
    20341 gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa cagttccgta
    20401 tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcaccag ctcaacatcg
    20461 ctcatgatgt tggccgccgc cacgcgccgc gcgtcctgct ggctgacgaa gtgggtttag
    20521 ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc gctgctgaac
    20581 gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa atgctgcgcc
    20641 gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct cagcacgatg
    20701 cttacaaccc gttcgatacc gaacagctgg tgatttgctc gctggatttt gcccgtcgta
    20761 gcaaacagcg tctggaacat ctctgtgaag ccgagtggga cctgctggtg gtcgatgaag
    20821 cccatcacct ggtgtggagc gaagatgcgc cgagccgcga atatcaggcc attgaacaac
    20881 tggcagagca cgtgccgggc gttctgctgc tgaccgcaac cccggaacaa ctggggatgg
    20941 aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat ttcgcgcaat
    21001 ttgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg ctgctggcag
    21061 gtaacaagct gagcaatgac gaactgaaca tgcttggcga gatgatcggc gagcaggata
    21121 ttgagccgtt gctgcaggcg gcaaacagcg acagcgaaga tgcacagagc gcccgtcagg
    21181 aactggtttc catgctgatg gatcgccatg gcaccagccg cgtgctgttc cgtaacaccc
    21241 gtaacggtgt gaaaggcttc ccgaaacgcg agctgcacac cattaagctg ccgctgccga
    21301 cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgcaaa agtgcggaag
    21361 atcgcgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa ggtgataacg
    21421 ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg accagccatc
    21481 gctctcagaa agtgctggtg atctgcgcta aagctgccac tgcgctgcaa ctggagcaag
    21541 tactgcgcga acgtgaaggt atccgcgctg ctgtgttcca cgaaggcatg tcgattatcg
    21601 aacgtgaccg cgctgccgcc tggtttgctg aagaagacac cggtgcgcag gtactgctgt
    21661 gctcggaaat cggttctgaa ggacgtaact tccagttcgc cagccacatg gtgatgtttg
    21721 acctgccatt caacccggat ctgctggagc agcgtattgg gcgtctggat cgtatcggtc
    21781 aggcgcacga tattcagatc catgtgcctt atctggaaaa aacggctcag tcggtgctgg
    21841 tgcgctggta tcacgaaggt ctggatgcat tcgagcacac ctgcccgacc ggacgcacta
    21901 tttacgatag tgtatacaac gatctgatta actatctggc ttcaccggat caaaccgaag
    21961 gctttgacga tctgatcaaa aactgccgcg agcaacatga agcgctgaaa gcacagctgg
    22021 aacaaggtcg tgaccgcctg ctggaaatcc attccaacgg tggcgaaaaa gcccaggcac
    22081 tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc gccatgaacc
    22141 tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc gtgctgacac
    22201 cgtccgatca tatgctggtg ccggacttcc ctggcttgtc ggaagatggc atcaccatca
    22261 ccttcgaccg tgaagtggcg ctggcgcgtg aagatgcgca gtttattacc tgggagcatc
    22321 cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc agcacgattt
    22381 cactgttgaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg atttacgtgg
    22441 tcgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccgccg acgccggtac
    22501 gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtggagttt gaaaccttta
    22561 accgtcagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac gccgtgcagc
    22621 aggatgttca cgctatcctt cagttgggtg aagcacagat cgagaaatct gcccgtgcac
    22681 tgattgatgc cgcacgtaac gaagccgacg aaaaactgtc tgccgagctg tctcgtctgg
    22741 aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc attgagagca
    22801 accgtcagca ggtaatggaa agcctggatc aggcaggctg gcgtctggat gccctgcgtt
    22861 tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact acaatccacc
    22921 gcaggaaccc tggctggtta tcctgtatca ggatgaccat attatggtgg tcaacaagcc
    22981 gagcggtttg ttgtctgtgc cgggtcgtct ggaagagcac aaagacagcg tgatgacgcg
    23041 cattcagcgt gattatccgc aggcggaatc ggtgcatcgt ctggatatgg ctaccagcgg
    23101 cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc agttccgcga
    23161 gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc ctgcggaagg
    23221 tctggtagat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga aagtctgtta
    23281 cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg cggataacac
    23341 ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg tgcatatgct
    23401 ggcgctgggt catccgattc tcggcgatcg tttttatgca tcaccggaag cgagagcgat
    23461 ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg cgtatggcaa
    23521 tagtatgacg tttaaagcgc cagcggattt ttaacctttc ctgtacgttt ataccctcat
    23581 cctaagggct gcctcttgta cataaatccc cgagtccggg gattttttgt ttctgccaaa
    23641 ctgctttcag ttgttgcgtg tggctacgct ttccattcgg taattccctt cctgctccac
    23701 agcgaatttt tgaacttttt cttcacccgt tgatcgtctc tattatttca ttttatcggg
    23761 tctgttatgg tcctttctga ttgttattca tgggctaatt agcagtctgg ttggcgatgc
    23821 ccatctcggt gatccgcgtc gtgcacgacg ccttgcatca ccagcctctt ctctagctca
    23881 tcatgcaggt ctttctaatt gtgaaatcat cccacgctac tgctaaggtt gaaggggcgt
    23941 tccagcccct tcaaccttag cttattctgg atcattcaat aattgcgttg gcaataattt
    24001 ttcagcagta ctttgtttag tagcaactcc ttcagtaaca attttttttg gggtgatttt
    24061 attaataata attacctcag catataggcg ggtagcacca tccacggtag caccgtccag
    24121 gataacaccg tccaggatag caccgtccag gttagcattc cacccaggtt ccaagtaaag
    24181 ggctttttta tctattattg tttttttacc aaagtaacaa tccctcatgt caacgccggg
    24241 aaggtaagcc tctccaagat caacgcccaa gttacctggg cctttatgca gctgcaacgt
    24301 ctgagtacat atctccttat tttcaatgaa aaaagtgaca cttaactcga tatcaccgta
    24361 gatctcggcg acccagcgca tcttgccctt tgcgaccatc tcatccggat tttttgcgca
    24421 tgttttgagt tcattgaata attccagact atggataaac ttttctttct cattctcgtc
    24481 gacagcccca gtaaagtatt ttgaggcgtt gatgttgcca gcctttttat tcacgaaatt
    24541 gaacagaagt ttataagcct cagtctgttt atctgaccag aacagatgac gtaacgtctc
    24601 ccacaagcgt tgaaagatat tcatcttcac gtccatcttg tccccgctca ccatcttata
    24661 aatatcgtta ttgctcatat ttaaaagtgt agccggcatg cctggaactg atactttcat
    24721 ggtaatagcc tcgcattgag ttaaaaactc atgatgcgcg ctctgaataa atatactttc
    24781 aaaaatcgct cattcagata tgtgttttta atttttaaga cgtaaaaata taattccgct
    24841 cgtcgtaaag ttctcaacct taagcagggt caacgcgata aaaccaacag aaaccctccc
    24901 gtaaaccgtg tattgagcct gtccaccact ctgagtaaac gccttttctc aaagaagtga
    24961 ccgccagggc ggccaccttt aatcgtgggc agttactcag catcatttac agggtcattt
    25021 aaaccctttc tgctgtttta tcagctcata ggcctgctga atttcctgcg ctttctgctt
    25081 cgccatctcc atcatctctg gcggcaaacc tttcgccacc agcttatcgg gatggtgttc
    25141 actcatcagc ttacggtagg cacgtttgat ggtggtcgca tcatccgtcg gcttcacgcc
    25201 cagcacatta caggcatctt ccagcgtcgg gccacgctgc gcttgctgcc agttaccacc
    25261 gccagattgc tgctgataac cgccgccaaa ctgcgccccg ccctgcatca tgcgcaaaaa
    25321 ctggtcaaac tgggcgcggg agatccctaa ttcttcagca atgacataca gcaccgcccg
    25381 ttcattcggg tgcagtgaac catcagcaaa cgccgcctga atctggatct ccagaaacat
    25441 acgaattaag tcaaaacgac caaagcaaac actgcgaaac tggcgcatct tttcgcgcag
    25501 cgggtaattg tctgattttc ccacccggaa cgcgttttgc gccgcagtac gggaagcgcc
    25561 atgcaggttc attcggtcca tcaactggct ggcgatatga atatcagcct ccgtgacgcg
    25621 acctttggat ttggttaaat gccccatcac ttcaaaagtg gtggcaaaaa acagcgcctg
    25681 acgctcacgc tggttggcga accacgccat tttacggcta cgggctttat caaacatatg
    25741 gccaattaac aggcctaaca ctacgcccca aaagccgccg cccatcagta aggccacggc
    25801 cacgccaatg atttttcccc aatactgcat atattcccca aatcgacaca cggatatcag
    25861 ggctatctcc cacaatataa aggtgctttt accgttttcc ggctgcggtc aattgtggga
    25921 catcgcctat aatttgcatt atcatacctg tcattcaatg ccgtgcctaa caccactgac
    25981 gctattcgga caggattaac actagcgtag agatgacgag tacgttagtc tctgaccgtt
    26041 tgtcacgcgc aacgttaccg atgatggaac aataaaatca acgtatgaaa aaacgtatcc
    26101 ccactctcct ggccaccatg attgccaccg ccctttatag tcaacaggga ctggcagccg
    26161 acctcgcctc acagtgcatg ttgggcgtgc caagctatga ccgtcctctg gtacagggcg
    26221 ataccaatga cttacccgtg actatcaatg ctgaccacgc gaaaggggac tacccggatg
    26281 acgccgtgtt tactggcagc gtggatatca tgcagggtaa cagccgtctg caggccgacg
    26341 aagtgcagct ccatcaaaaa gaggcaccag gacaaccgga gccggtacgt accgttgatg
    26401 cgctcggtaa tgtccattac gacgataacc aggtgatcct caaagggccg aaaggctggg
    26461 cgaatctgaa caccaaagat accaatgtct gggaaggtga ttaccagatg gtgggtcgtc
    26521 agggccgcgg taaagcggac ctgatgaaac aacgtggcga aaaccgctat accattctgg
    26581 ataacggtag ctttacctcc tgtctgccgg gttctgacac ctggagcgtg gtaggtagcg
    26641 aaattattca tgaccgcgaa gaacaagtgg cggagatctg gaacgcccgc tttaaggtgg
    26701 gtccggtacc gatcttttat agcccctatt tgcagttgcc ggtgggtgac aaacgtcgct
    26761 ctggtttctt gatcccgaac gccaagtaca ccaccaccaa ctactttgag ttctacctgc
    26821 catattactg gaacatcgcg ccaaatatgg atgccaccat cacgccgcat tatatgcatc
    26881 gtcgtggcaa catcatgtgg gagaacgaat tccgctacct ctcccaggcg ggcgctggct
    26941 tgatggaact ggactatctg ccttcagata aagtctatga agatgaacat ccgaacgatg
    27001 acagttcacg tcgttggttg ttctactggc aacactccgg ggtcatggat caggtgtggc
    27061 gtttcaacgt cgactacacc aaggtcagcg atcctagcta cttcaatgat ttcgataaca
    27121 agtacggttc cagtactgac ggctacgcaa cgcaaaaatt cagcgttggc tatgcggtgc
    27181 aaaacttcaa tgccaccgtt tcaaccaagc agttccaggt cttcagcgag cagaacacca
    27241 gtagctactc ggcagagccg cagttagacg ttaactacta ccagaatgat gttggtccgt
    27301 ttgatacacg tatttacggc caggcagtgc actttgttaa caccagagac gacatgcctg
    27361 aagcaacccg tgttcacctg gaaccgacca tcaatttgcc gctctctaat aactggggca
    27421 gcatcaatac cgaagcgaag ttgctggcaa cccattatca gcaaactaat cttgactggt
    27481 ataactccag aaacacaacc aagctggacg aatccgttaa ccgcgtaatg ccgcaattca
    27541 aagttgacgg caaaatggtc tttgaacgcg atatggaaat gctggctccg ggttataccc
    27601 aaacgctgga accgcgcgcg cagtatttat acgtgccgta tcgcgatcag agcgacatct
    27661 ataactacga ctcgtctctg ctgcaatctg actactctgg cctgttccgg gaccggactt
    27721 acggcggtct tgaccgtatt gcctccgcta accaggtgac gaccggtgtc acatctcgca
    27781 tatatgatga tgctgccgtt gaacgtttta atatttccgt tggtcaaatc tactatttta
    27841 cggagtctcg cactggcgat gacaacataa catgggagaa tgacgacaaa acgggctcac
    27901 tggtgtgggc aggcgatact tactggcgta tctccgagcg ttggggattg cgtggcggga
    27961 ttcagtacga tacacgtctg gataacgtag cgaccagtaa ctccagcatt gaataccgtc
    28021 gggatgaaga ccgtctggta cagctgaatt accgttacgc cagcccggaa tatattcagg
    28081 ctacgctgcc taagtactat tccacagctg agcaatataa gaatggtatt tcgcaggtag
    28141 gtgccgtcgc cagctggcca attgctgatc gttggtcaat tgttggggcc tactactacg
    28201 acaccaatgc gaacaagcaa gccgactcta tgttaggtgt gcaatacagc tcctgctgct
    28261 atgcaattcg cgtcggttac gagcggaagc tgaacggttg ggataacgat aaacaacatg
    28321 cggtatatga caacgcaatc ggctttaaca tcgaacttcg cggcctgagc tccaactacg
    28381 gtctgggtac gcaagagatg ctgcgttcga acattctgcc gtatcaaaac actttgtgat
    28441 ttgttgattt accacgtaat ccgcagtgcg gttaattgaa atggaaaaag tatgaagaac
    28501 tggaaaacgc tgcttctcgg tatcgccatg atcgcgaata ccagtttcgc tgccccccag
    28561 gtagtcgata aagtcgcagc cgtcgtcaat aacggcgtcg tgctggaaag cgacgttgat
    28621 ggattaatgc agtcggtaaa actgaacgct gctcaggcaa ggcagcagct tcctgatgac
    28681 gcgacgctgc gccaccaaat catggaacgt ttgatcatgg atcaaatcat cctgcagatg
    28741 gggcagaaaa tgggagtgaa aatctccgat gagcagctgg atcaggcgat tgctaacatc
    28801 gcgaaacaga acaacatgac gctggatcag atgcgcagcc gtctggctta cgatggtctg
    28861 aactacaaca cctatcgtaa ccagatccgc aaagagatga ttatctctga agtgcgtaac
    28921 aacgaggtgc gtcgtcgcat caccatcctg ccgcaggaag tcgaatccct ggcgcagcag
    28981 gtgggtaacc aaaacgacgc cagcactgag ctgaacctga gccacatcct gattccgctg
    29041 ccggaaaacc cgacttctga tcaagtgaac gaagcggaaa gccaggcgcg tgccattgtc
    29101 gatcaggcgc gtaacggcgc tgatttcggt aagctggcga ttgctcattc tgccgaccag
    29161 caggcgctga acggcggcca gatgggctgg ggccgtattc aggagttgcc ggggatcttc
    29221 gcccaggcat taagcaccgc gaagaaaggc gacattgttg gcccgattcg ctccggcgtt
    29281 ggcttccata ttctgaaagt taacgacctg cgcggcgaaa gcaaaaatat ctcggtgacc
    29341 gaagttcatg ctcgccatat tctgctgaaa ccgtcgccga tcatgactga cgaacaagcc
    29401 cgcgtgaaac tggaacagat tgctgccgat atcaagagtg gtaaaacgac ttttgctgcc
    29461 gcagcgaaag agttctctca ggatccaggc tctgctaacc agggcggtga tctcggctgg
    29521 gctacgccag atattttcga tccggccttc cgtgacgccc tgacccgcct gaacaaaggt
    29581 caaatgagtg caccggttca ctcttcattc ggctggcatt taatcgaact gctggatacc
    29641 cgtaatgtcg ataaaaccga cgcggcgcag aaagatcgtg cgtaccgcat gctgatgaac
    29701 cgtaagttct cggaagaagc agcaagctgg atgcaggaac aacgtgccag cgcctacgtt
    29761 aaaatcctga gcaactaatg gttaaaaccc aacgtgttgt gatcactccc ggcgagcccg
    29821 ccgggattgg cccggactta gttgcccagc ttgcacagcg tgagtggccg gtcgaactgg
    29881 ttgtttgtgc cgatgccact ctccttaccg accgggcagc gatgctcggt ttgacgctca
    29941 ccctccgccc ttattccccc acctcccctg cacaaccgca aactgcgggc acattaacgc
    30001 tacttcctgt cgcgctacgt gaatctgtca ctgcggggca gttagcggtt gaaaatgggc
    30061 attatgtggt ggagacgctg gcgcgagcgt gtgatggctg tctgaacggt gaatttgctg
    30121 cgctgatcac aggccccgtg cataaaggcg tcattaacga cgcaggcatt ccgtttaccg
    30181 gtcataccga gtttttcgaa gagcgttcgc aggcgaaaaa agtggtgatg atgctggcga
    30241 ccgaagaact tcgcgtggcg ctggcaacga cgcatttacc gctgcgcgat atcgcagatg
    30301 ctatcacccc tgcgcttttg cacgaagtga ttgctatttt gcatcacgat ttgcggacca
    30361 aatttggtat tgccgaaccg cgcattctgg tctgcgggct gaatccgcac gcgggcgaag
    30421 gcggtcatat gggtacggaa gagatagaca ccattattcc ggtgctcgac aagctgcggg
    30481 tgcaggggat gaaactcaac gggccgctgc ctgccgatac cctgtttcag ccgaaatatc
    30541 tcgataacgc cgacgccgtg ctggcgatgt accacgatca gggtcttccc gtgctaaaat
    30601 accagggctt cgggcgcggt gtgaacatta cgctgggcct gccctttatt cgcacatcag
    30661 tggaccacgg caccgcgctt gaactagcgg gacgtggtaa agccgatgtc ggcagtttta
    30721 ttacggcgct taatctcgcc atcaaaatga ttgttaacac ccaatgaata atcgagtcca
    30781 ccagggccac ttagcccgta aacgcttcgg gcaaaacttt ctcaacgatc agttcgtgat
    30841 cgacagcatt gtttctgcca ttaacccgca gaagggccag gcgatggtcg aaatcggtcc
    30901 cggtctggcg gcattgaccg aaccggtcgg cgaacgtctg gaccagctga cggtcatcga
    30961 acttgaccgc gatctggcgg cacgtctgca aacgcatcca ttcttaggcc cgaaactgac
    31021 gatttatcag caggatgcga tgacctttaa ctttggcgaa ctggccgaga aaatgggtca
    31081 gccgctgcgt gttttcggca acctgcctta taacatctcc acgccgttga tgttccatct
    31141 gtttagctat actgatgcca ttgccgacat gcactttatg ttgcaaaaag aggtggtgaa
    31201 tcgtctggtt gcaggaccga acagcaaagc gtatggtcga ttaagcgtca tggcgcaata
    31261 ctattgcaac gtgatcccgg tgctggaagt tccgccgtca gcctttacac caccacccaa
    31321 agtggattcc gccgtcgtgc gcctggttcc tcatgcaacg atgcctcacc cggttaaaga
    31381 tgttcgcgtg ttgagccgca tcaccaccga agcctttaac cagcgtcgta aaaccattcg
    31441 taacagcctc ggcaacctgt ttagcgtcga ggtgttaacc ggaatgggga tcgacccggc
    31501 gatgcgagcg gaaaatatct ctgtcgcgca atattgccag atggcgaact atctggcgga
    31561 gaacgcgcct ttgcaggaga gttaacgatg atcaattcgc cccgagtgtg tattcaggtt
    31621 caaagcgtct acattgaggc acaatcttca cctgataatg aacgttacgt ttttgcttat
    31681 accgtaacca tacgcaatct ggggcgagcg ccagtgcagt tgttggggcg ttactggctg
    31741 atcaccaatg gcaatggccg tgaaaccgaa gtccagggcg aaggagtggt tggcgtccag
    31801 ccacttatcg cgcctggcga agagtaccag tacaccagcg gtgcaatcat tgaaaccccg
    31861 ctgggcacca tgcagggtca ctacgaaatg atcgatgaaa atggcgtccc tttcagcatc
    31921 gacattcccg tattccgact cgccgttccc acactcattc attaaaagaa tatggcgaca
    31981 taccttattg gcgacgttca tggttgttac gatgaactga tcgcattgct gcataaagta
    32041 gaatttaccc ctgggaaaga taccctctgg ctgacgggcg atctggtcgc gcgcggcccg
    32101 ggttcgctgg atgttctgcg ctatgtgaaa tccttaggcg acagcgtacg tctggtgctg
    32161 ggcaatcacg atctgcatct gctggcggta tttgccggga tcagccgcaa taaaccgaaa
    32221 gatcgcctga caccgctgct ggaagcgccg gatgccgacg agctgcttaa ctggctgcgt
    32281 cgccagcctc tgctgcaaat cgacgaagag aaaaagctgg tgatggccca cgccgggatc
    32341 acgccacagt gggatctgca gaccgccaaa gagtgcgcgc gcgatgtaga agcggtgctg
    32401 tcgagtgact cctatccctt ctttcttgat gccatgtacg gcgatatgcc aaataactgg
    32461 tcaccggaat tgcgggggct gggaagactg cggtttatca ccaacgcctt tacccgtatg
    32521 cgtttttgct tcccgaacgg tcaactggat atgtacagca aagaatcgcc ggaagaggcc
    32581 cctgccccac tgaaaccgtg gtttgcgatt cctggccctg tcgctgaaga atacagcatc
    32641 gcctttggtc actgggcatc gctggaaggc aaaggtacac cggaaggtat ttacgcgctg
    32701 gatactggct gctgctgggg tggtacatta acctgcctgc gctgggaaga taaacagtat
    32761 tttgtccagc cgtcgaaccg gcataaggat atgggcgaag gagaggcggt cgcgtcttaa
    32821 atcccgtcga gcataaaatc taatgcccct tcaatcaacg ttcggtgcgc gctggcatct
    32881 caaaatttcg ttcccagcgc gttaactgga acagttgcag tctcatgcgt catgagcaca
    32941 tattctttgc catctaccag taaaagaatt gggttaagcc gttctggtgg gcgaatacgg
    33001 ctaaatcgtt caataggtgt taatggaata acaatacggc gattaatctc accaataatg
    33061 tcgctggtaa catcgataac aaagggaaac gcggcgttcc tgccgcgact gcggtatacc
    33121 gtaaattgca tcgctagaag ctccggtact catcagagaa acatccggtt tcatcagcca
    33181 attgatttaa cgcagcgata gcttctgcgt tctcttcacg ccaacgtgtt gctgcatgtt
    33241 ttttaagttc agcattgagc gcaaccataa gggtggcgct catatttaag cctgcatcac
    33301 gagcgcgatt gactaactca cggtcgacgg tgacggtcac actttgtgtg gtacgttttg
    33361 cagtcatgat atcgtgctcc tgtacaattt atacgtctag taaacacgga tatctgcacg
    33421 tatcagtgat gccggaattc taatatacaa acttaccgcc gctccagaat ctcaaagcaa
    33481 tagctgtgag agttctgcgc atcagcgtcg tggaattcgc tgaataccga ttcccagtca
    33541 tccggctcgt aatccgggaa atgggtgtcg ccttccacct ctgcgtcgat atgcgtcaga
    33601 tacagttttt gcgcttttgg caggaactgc tcataaacgc gaccaccgcc aatcaccatg
    33661 atttctggta cgtcaccaca cgccgcgatg gcttcatcca ccgatttcac ccacgttacg
    33721 cgatcgtccg tacccggttg actgctgagg ataatatttt tgcgtcctgg cagcggacga
    33781 ccgatggatt cccaggtatg gcggcccata attacgggtt tatttaaagt gttgcgttta
    33841 aaccaggcga gatcggcagg caggttccac ggcatggcgt tttccatgcc gataacgcga
    33901 tctacagcta acgccgcaat cagactgatc attgagattt cccgataaaa aaaattgtcg
    33961 ccactatacg taaagcgtaa accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt
    34021 ttatattctg ctggcgagtc cacgctctct ccctggactc gcc
//
LOCUS       DAPOZO010000051        33731 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPOZO010000051 DAPOZO010000000
VERSION     DAPOZO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.051"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(9..2078)
                     /gene="glyS"
                     /locus_tag="ROI21_002622"
     CDS             complement(9..2078)
                     /gene="glyS"
                     /locus_tag="ROI21_002622"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDX5111530.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGA
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARYDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2088..2999)
                     /gene="glyQ"
                     /locus_tag="ROI21_002623"
     CDS             complement(2088..2999)
                     /gene="glyQ"
                     /locus_tag="ROI21_002623"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDX5111531.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3094..3402)
                     /locus_tag="ROI21_002624"
     CDS             complement(3094..3402)
                     /locus_tag="ROI21_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDX5111532.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     GLCKDQAAKRYNTGAQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            3577..4572
                     /gene="wecH"
                     /locus_tag="ROI21_002625"
     CDS             3577..4572
                     /gene="wecH"
                     /locus_tag="ROI21_002625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDX5111533.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVCAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICATTLLTLVKNTLDTRTVRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4614..5051)
                     /locus_tag="ROI21_002626"
     CDS             complement(4614..5051)
                     /locus_tag="ROI21_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDX5111534.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQITQEEYSE"
     gene            complement(5097..5438)
                     /locus_tag="ROI21_002627"
     CDS             complement(5097..5438)
                     /locus_tag="ROI21_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111535.1"
                     /translation="MKTSKTVAKILFVVGALVYLLGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRPA"
     gene            complement(5606..7060)
                     /gene="xylB"
                     /locus_tag="ROI21_002628"
     CDS             complement(5606..7060)
                     /gene="xylB"
                     /locus_tag="ROI21_002628"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDX5111536.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRLLRPAILWNDGRCA
                     QECALLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLNNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQRLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7132..8454)
                     /gene="xylA"
                     /locus_tag="ROI21_002629"
     CDS             complement(7132..8454)
                     /gene="xylA"
                     /locus_tag="ROI21_002629"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDX5111537.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFHHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8820..9812
                     /gene="xylF"
                     /locus_tag="ROI21_002630"
     CDS             8820..9812
                     /gene="xylF"
                     /locus_tag="ROI21_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDX5111538.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAIFGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKETVVKDGFHKESEL"
     gene            9890..11431
                     /gene="xylG"
                     /locus_tag="ROI21_002631"
     CDS             9890..11431
                     /gene="xylG"
                     /locus_tag="ROI21_002631"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDX5111539.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLVQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11409..12590
                     /gene="xylH"
                     /locus_tag="ROI21_002632"
     CDS             11409..12590
                     /gene="xylH"
                     /locus_tag="ROI21_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDX5111540.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12668..13846
                     /gene="xylR"
                     /locus_tag="ROI21_002633"
     CDS             12668..13846
                     /gene="xylR"
                     /locus_tag="ROI21_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDX5111541.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(13954..14778)
                     /locus_tag="ROI21_002634"
     CDS             complement(13954..14778)
                     /locus_tag="ROI21_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDX5111542.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15098..17128
                     /gene="malS"
                     /locus_tag="ROI21_002635"
     CDS             15098..17128
                     /gene="malS"
                     /locus_tag="ROI21_002635"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDX5111543.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGEGKQTTLSMKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17306..18559
                     /gene="avtA"
                     /locus_tag="ROI21_002636"
     CDS             17306..18559
                     /gene="avtA"
                     /locus_tag="ROI21_002636"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDX5111544.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18711..19184)
                     /locus_tag="ROI21_002637"
     CDS             complement(18711..19184)
                     /locus_tag="ROI21_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDX5111545.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRNRLRQQPV"
     gene            complement(19286..20134)
                     /gene="yiaJ"
                     /locus_tag="ROI21_002638"
     CDS             complement(19286..20134)
                     /gene="yiaJ"
                     /locus_tag="ROI21_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDX5111546.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20336..21334
                     /gene="yiaK"
                     /locus_tag="ROI21_002639"
     CDS             20336..21334
                     /gene="yiaK"
                     /locus_tag="ROI21_002639"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDX5111547.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21346..21813
                     /locus_tag="ROI21_002640"
     CDS             21346..21813
                     /locus_tag="ROI21_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDX5111548.1"
                     /translation="MIFGHIAQPDPCRLPAAIEKGLDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHCGYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYVIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            21931..22404
                     /gene="yiaM"
                     /locus_tag="ROI21_002641"
     CDS             21931..22404
                     /gene="yiaM"
                     /locus_tag="ROI21_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDX5111549.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22407..23684
                     /gene="yiaN"
                     /locus_tag="ROI21_002642"
     CDS             22407..23684
                     /gene="yiaN"
                     /locus_tag="ROI21_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDX5111550.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLEMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            23697..24683
                     /gene="yiaO"
                     /locus_tag="ROI21_002643"
     CDS             23697..24683
                     /gene="yiaO"
                     /locus_tag="ROI21_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDX5111551.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITVLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            24687..26183
                     /gene="lyxK"
                     /locus_tag="ROI21_002644"
     CDS             24687..26183
                     /gene="lyxK"
                     /locus_tag="ROI21_002644"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDX5111552.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAEQWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITCGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQGDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            26180..26842
                     /gene="ulaD"
                     /locus_tag="ROI21_002645"
     CDS             26180..26842
                     /gene="ulaD"
                     /locus_tag="ROI21_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDX5111553.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVMQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPNKLIVADWKVADAGETLAQQAFTAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPVQVAGDFHAQIDAIWGGK
                     RA"
     gene            26835..27695
                     /locus_tag="ROI21_002646"
     CDS             26835..27695
                     /locus_tag="ROI21_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDX5111554.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27689..28384
                     /gene="araD"
                     /locus_tag="ROI21_002647"
     CDS             27689..28384
                     /gene="araD"
                     /locus_tag="ROI21_002647"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDX5111555.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYNVMTADDMVVVEIATGKVVEGRKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28416..29231)
                     /locus_tag="ROI21_002648"
     CDS             complement(28416..29231)
                     /locus_tag="ROI21_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX5111556.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     AIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVADPQVLVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            29457..30857
                     /locus_tag="ROI21_002649"
     CDS             29457..30857
                     /locus_tag="ROI21_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5111557.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGIIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTTSSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            30868..32838
                     /locus_tag="ROI21_002650"
     CDS             30868..32838
                     /locus_tag="ROI21_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDX5111558.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LVASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRISGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVGQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(32968..33708)
                     /locus_tag="ROI21_002651"
     CDS             complement(32968..33708)
                     /locus_tag="ROI21_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDX5111559.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMVDRNESFTFVTGVSWRF"
BASE COUNT         8217 a         8757 c         8514 g         8243 t
ORIGIN      
        1 cggcgttatt actgtaacag cgaaatatcc gcaacgcgca ggaacagttc gcgcagtttc
       61 tccagcatgg tcaaacggtt gatacgcaat tctttgtcat caaccatgac catcacttta
      121 tcgaagaagg catcaaccgg ttcacgcagc tcagccagtt cgaccagcgc atcctggtaa
      181 cgaccttcag caaagtacgg ctccagttta tcgcgcagca caaccacctg catcgccagt
      241 ttaatttctt ccggctcttt cagagtagaa gcattcacgc gatcgctcag cacttcgtca
      301 gacttcgcca gaatgttaga tacacgtttg ttcgccgccg ccagtgcagc agctgcatcc
      361 agggtacgga agtgcgatac cgccttcata cgggcatcga aatctgccgg acgagtcgga
      421 cgacgcgcca gtaccgcctg gatggtgtca acggtgtaac cttcgtcctg ataccaggcg
      481 cggaagcgac cgagcataaa gtcgataaca tcatcaacca cgttggcatt agtcagctta
      541 tcgccataca gacgcactgc ttcttcggtc agcgtttgca gatcaaggtt gaggttcttc
      601 tcaacgataa tacgcagtac gccaagtgcg gcacgacgca acgcaaacgg gtctttgtcg
      661 cctttcggat gctggccgat accgaagata cccgccagag tatccatctt gtcagcaatc
      721 gccagcgcac aggctaccgg gttagacggc aggtcatcac cggcaaagcg cggctgatac
      781 tgctcgttca gcgccactgc aacatcttcc gcttcgccat cgtaacgcgc gtagtgcatc
      841 cccataacgc cctgggtgtc ggtgaactcg aagaccatgt tggtcatcag gtcgcacttg
      901 gacagcaggc ccgcacgggt tgcgtggtta acgtcagcgc caatctgttc agcaatccag
      961 ccagccagcg cctggatgcg gtcagttttg tcgcgcagtg tacccagctg ttgctgaaac
     1021 aacacggttt gcaggcgcgg caggttatct tccagacgtt ttttacggtc ggtgttgaag
     1081 aagaactcgg catccgccaa acgtggacga acgactttct cgttaccaga gataatttgc
     1141 tgcggatctt tcgattcgat attggcaacg aagataaagt tcggcagcag tttgccgtcg
     1201 ttcgcataca ccgggaagta tttctggtca cctttcatgg tgtaaaccag cgcttcagac
     1261 ggcaccgcaa ggaatttctc ttcgaatttt gcggtcagca caaccggcca ttccaccagc
     1321 gaagccactt cttccaacag gctttcgctt aagtcagcgt taccgccaat cttacgcgcc
     1381 gcttcttcgg catcggcttt aatcttcgcc ttacgttctt cgtaatcggc gatgactttc
     1441 ccgcgctcac gcagaatttc cggatactga tcggcattgt cgatagtgaa ttccggctcg
     1501 cccataaagc ggtggccgcg aatcacgcga tcggactgaa tgcccagaat ggtagccgga
     1561 ataactttgt cgcccagcag cagggtcacg gtgtgcaccg gacgcacgaa gtgcacgtcg
     1621 cttgcgcccc aacgcatcag tttcgggatc ggcagtttcg ccagcgaagt cgcaaccata
     1681 ttcggcagca gtgcttcggt gctttcgccc ttcacgtggg cgcgatacag cagccattcg
     1741 cctttatcgg tagtcagacg ctcagcctgg tcaacggtaa taccgcaacc acgcgcccag
     1801 ccttctgccg ctttgctcgg tttgccttca gcgtcgaacg cctgagcaat cgccgggccg
     1861 cgtttttcga tttcacgatc cggttgcgct tccgccaggt tagctacttt cagcgccaga
     1921 cgacgcggag cagcaaacca ttgaacggcg ccgtgtgcga ggccagcgtt atccagctcc
     1981 gcagtaaagt tcgcagcaaa ggactcagcc aggctgcgca gtgcttttgg tggcagctct
     2041 tcagtgccga tttccaccag aaaagttttc tcagacatag ccgcctctta cttatctttg
     2101 ttgcacatcg ggaagccgag ggcttcacgg gaagcgtagt atgcttctgc cactgctttg
     2161 gtcagggtgc gaatgcgcag aatatagcgc tgacgctcag tgacggagat ggctttacgc
     2221 gcatccagca ggttgaagct gtgggcggct ttcagaatac gctcgtaggc tggcaacggc
     2281 agcggatttt ccagcgccag cagctgctgc gcttctttct cgtactgctc gaagcaggtg
     2341 aacaggaagt ccacatccgc gtattcgaag ttgtaagtgg actgctccac ttcgttctga
     2401 tggaacacgt cgccataggt ggttttaccc agcgggccgt cgctccagac caggtcgtaa
     2461 acgctgtcta cgccctgaat gtacattgcc agacgttcca gaccgtaggt gatctcgccg
     2521 gtaaccggtt tacactccag accaccaacc tgctggaagt aagtgaactg cgtcacttcc
     2581 atgccgttca gccacacttc ccagcccagc ccccaggcac ccagcgtcgg gttttcccag
     2641 ttatcttcca cgaaacggat gtcgtggatg gtcgggtcca tgcccagctc tttcagagaa
     2701 ccaaggtaca gctcctgaat attgtccggc gatggcttaa tgaccacctg gaactgatag
     2761 tagtgctgta aacggttggg gttttcgccg tagcgaccat cggttgggcg acgagaaggc
     2821 tgaacataag cagccgccat cggttctggc cccagcgcgc gcagacaggt cattgggtga
     2881 gaggttcccg cgccgacttc catgtccaat ggttgaacaa tggtgcagcc ctggcgagcc
     2941 cagtaatcct gtaaggtcag gatcaagccc tggaaggtcc tggtatcaaa cttttgcata
     3001 ttatttcgtg ctggatacgt gtggatttaa aggaagggat cagtataccc gctggatgga
     3061 agatatacag tacgaaacgg gaaaaagcag ggcttaacgc atggaaagat gcaaaaaatg
     3121 gccatctgcg tcaaaagaac agacaaactg ctctttacgg aaggtataac cgcgcatttc
     3181 gtagctcccc tggaactgtt caaatgcggt gacgtcgatt ttttgcgcac ctgtattata
     3241 gcgttttgcc gcctgatctt tgcatagccc ttccatattc aacgaacgca gaggatcaac
     3301 ctttacccgt tgtgctttct gcacgggtgg cggtgtagaa caacccacta gcgccataag
     3361 caccataagc gccattgccg gaaagaaaga gttcatcatc attaaattac cgtctggttt
     3421 gcaggtgagt cttattattt atatcggtat agtggctgat atcttacgaa tttccttaag
     3481 cctctataag ctgtagtgat aaaactcaga ttattccagt gcgccaaccg aatgatttat
     3541 cggtcagact ttttttaacc agatacagag acacagatgc agcccaaaat ttactggatt
     3601 gataacctgc gagggatagc gtgtttaatg gtggtgatga ttcacaccac tacctggtat
     3661 gtgaccaatg ctcatagtgt tagccccgtc acatgggata tcgccaatgt tctgaattct
     3721 gcctctcgtg tcagcgtgcc gctatttttc atgatttccg gctatctctt ttttggcgaa
     3781 cgcagcgccc agccgcgcca tttcttgcgt atcggcttat gtctgctttt ttatagcgca
     3841 atcgcactgc tctacattgc gctgtttacc tccatcaata tggagttagc gctgaaaaac
     3901 ctgctgcaaa agccagtgtt ttaccacttg tggtttttct tcgcgattgc ggtgatttat
     3961 ctggtttcac cgctgattca ggtgaagaac gtcggcggaa aaatgttgct ggtgctaatg
     4021 gtggtgattg gcattatcgc taacccaaac acagtgccgc agaaaattga cggttttgaa
     4081 tggctgccaa ttaacttata tatcaatggc gatacttttt actacatcct gtatggcatg
     4141 ttgggccgcg ctatagggat gatggacaca cagcataaag cactgtcgtg ggtgtgcgcc
     4201 gcactgtttg cgacgggggt ttttattatc tctcgcggga cattatatga attgcagtgg
     4261 cgcggaaatt ttgccgatac ctggtatctt tactgtgggc cgatggtttt tatctgcgca
     4321 accacgctat tgactctggt taaaaacacg cttgatacgc gtactgttcg cggacttggc
     4381 ttaatctccc gccattcgtt gggtatatac ggattccacg ccttgattat ccatgcactg
     4441 cgcacccggg ggattgagct taaaaactgg ccaatactcg atattatttg gatcttttgc
     4501 gcgacgttgg cagcgagttt gttactttct atgctggtac aacgaatcga cagaaacaga
     4561 ctagtgagtt aaataaaagc ccggtcacat tggactgacc gggcttatgt gaattattcg
     4621 ctgtattctt cctgggtaat ctgtgtttct tttggtgggt ttattccggc atcacgaata
     4681 ttcttctgca ccgctactgc accaaacaag cttaagaaga aagccagtcc ataaaaacct
     4741 ttctcgctga gtaataacgt cgcattccac agacctacca tcagtaacgc aacagagata
     4801 ataaagacag tcaggcaggt catataataa atggaggtgg tcggtatgcc ttcatacttg
     4861 tcccgaacgg tcttttgata agacgccgca gaaaacagtc ctaataccag tacggcaaaa
     4921 taatatcctt tttcatttaa ctgcatctct gcattccata gccctaacag ataggtaacg
     4981 ataccaccaa cgagagctat ccatgacaca atactgaatg ccggagaata ggttgatatt
     5041 ttgttgtcca tcacagtatt cctttcattc ctgaatgtgt aagagcttta tgttgctcat
     5101 gccgggcgaa acaaacgcat ttgaccaaac aagcagacaa aaaaggcgat agggtagatc
     5161 accatttcat aagcattgat aggtgcgttt aaaacaccga taaacaagag accgatagtg
     5221 attaacgcaa ctaactggca gatgtgggta aaaaaatcat ccagttgccc gagtttttct
     5281 gcgcgaagat atgcatagtt gccaaaagtt gccgtcatta acacgccaag aaaatagcct
     5341 tttccactta acaatgggca tgagatccat agcccaagca gataaaccag cgccccgaca
     5401 acaaataata tttttgccac agtctttgag gttttcataa cgatctccat atctaccagc
     5461 gatacattac gagtaaccaa cgaaagacaa aactgaaaaa tgccattaac aaatgatttc
     5521 agaataaatt catactaaat attaattaat tactgatata tatagatgtg aattatcccc
     5581 cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc tgatagaggc
     5641 gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct ggtagatgcg
     5701 actgttctaa cggcagttgc ggcaacaact caatgagcga tttctctgga ttcgccgcaa
     5761 tctgcgccag ccttgctgcg cccagtgctg gccccacatc cccccccgta cggtaatcga
     5821 gccgctgacc gctgatatcc gccagcatct gacgccagta ctcactacgc gccccgccac
     5881 caatcaacgt aacactttgc ggtttaatgc cgcaggcatg cacgacatcc atgccatctg
     5941 ccagcgcata acccacgcct tccagcactg ctcgcgccag ttcattgggg ccatgttgat
     6001 gagtcaaacc aaagaaaacc cccttcgcct ggggattatt gtgtggtgta cgctcgccgg
     6061 aaagataagg cagaaaccaa actggctcgg cactttcatc agcctgttga gcagccgcga
     6121 ttaaagctgg gacattgttc aggcccgtta atttcgcagc ccaatccaga cacgacgctg
     6181 cactcagcat cacagacatt aaatgccaac gctgcggtag cgcatggcaa aagctgtgta
     6241 cggcgctttc tggcttgctt aagaaccctt cgctgacagc aaaatagacc cccgacgtcc
     6301 ccagcgataa cattgcctga ttagcatcaa ccattcctac accaaccgca ccagctgcat
     6361 tatcaccacc gcctgcgaca actggcaccg ccgccatacc ccacgctttc gcaacttcag
     6421 gtaacaaagc accagtaatt tcgctgcctt cgtataatgc gggcatctgg tcacgagata
     6481 agtggcaagc ctgcagcatg acgtcactcc agtcacgctt tgcgacatcc agccacatgg
     6541 tgccagctgc gtcagacata tcgctggcaa actcccccgt catacgcaga cgcaagtaat
     6601 ctttcggtaa caatactttg tcgatttgac ggaatatctc cggctcatgc cgctgaaccc
     6661 atagcaattt aggcgcagta aatccgggca tcatcaggtt accggtaatc acccgcgatt
     6721 gcggaactcg cgcttccagc aaagcgcact cttgcgcaca gcgcccgtcg ttccacaaaa
     6781 tggcagggcg taataaccgt tgctgggcat ccagtaaggt tgcaccgtgc atctggccgg
     6841 caatacccaa tgctttaacg tcctgcagag aatgctgatc gcccagggct ttcattgcgc
     6901 gatcagttgc ctgccaccac tgttccgggt cttgttccga ccagagtgga tgcgggcgcg
     6961 aaacggtcag cttttccgtt tgcgaagcaa ccacctcgcc ctgctcgttg agcaaaataa
     7021 cttttacgcc cgaggtgcca agatctatcc cgatatacat atcgatcgtt ccttaaaaaa
     7081 atgcccggta tcgctaccga taaccgggcc aacggactgc acagttagcc gttatttgtc
     7141 gaacagataa tggtttacca gattttccag ttgctcctgg cgaccactct gatgcaccgg
     7201 agacaaattg tgttcctgag catatttggc taaatctgcc agtgacattt ggcctttcag
     7261 gatttgctgg cctaattcgc tattccagcc ggaataacgc tgcgcgatgc gtttatccag
     7321 ctcaccatct tcaatcatgc gtgctgcaat tttcagcgcc aacgccatcg tatccatcgc
     7381 gccgatatga ccgtaaaaca gatcatattt atcagtactt tgacgacgta ctttggcatc
     7441 gaagttcaga ccgccggtgg tgaaaccgcc tgctttgaga atttcataca tcaccagcgc
     7501 attctcttcc acactgttcg ggaactggtc ggtatcccag cccagttgtg catcgccacg
     7561 gttagcgtcg acagaaccga acaggccaag cgcaatggcg gtggctattt catgatggaa
     7621 agagtgacct gccagcgtcg cgtggttagc ttcaatgttc agtttaatct ctttttccag
     7681 accaaactgt ttcaggaagc catagactgt cgcagcatcg taatcatatt gatgtttggt
     7741 cggttcttgc ggtttcggtt cgataagcaa cgtaccctgg aagccgattt tatgtttatg
     7801 ctcaaccacc atctgcataa agcggcccag ttgttcacgc tcctgacgca ggtcggtatt
     7861 taacagcgtt tcgtaacctt cacgaccgcc ccacaggaca tagttttcac cacccaattt
     7921 atgggttgct tccatcgctg taacaacttg cgttgccgcc cagctgaaga cttcaggatc
     7981 tgggttcgtc gccgcacccg cgccgtagcg agggtttgta aagcagttag cggttcccca
     8041 cagcagcttc acgccgctct cttcttgctt gcctgccagg acatcaacca tttgcgcaaa
     8101 attattgatg tactctttta acgacgcgcc ctcaggggaa acatccacgt cgtggaagca
     8161 ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg acatctgctt tacgcttcgc
     8221 caacgccagt gcctcaccag gctgctgcca cggacgatta aacgccccca caccaaacat
     8281 gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg gcaaatcgca agtgctcttc
     8341 catacgcttg cccaacacca gttcgtcggg attatagtga tggaatgcta acgggttaga
     8401 ggattttgag ccttcataac gaacgcgatc gagctggtca aaataggctt gcatattgaa
     8461 ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg taatattggg cactcccttt
     8521 cagttgctca attatgttat ttcacactgc tattgagata attcacaagt gtgcgctcgc
     8581 tcgcaaaata aaatggaatg aggaaactgg gtaattcctc gaagagaaaa atgcaataag
     8641 tacaattgcg caacaaaagt aagatctcgg tcataaatca agaaataaac caaaaatcgt
     8701 aatcgaaaga taaaaatctg taattgtttt cccctgttta gttgctaaaa attggttacg
     8761 tttatcgcgg tgattgttac ttattaaagc tgtcctctac ctacagaagg ccctacacca
     8821 tgaaaataaa gaacattcta ctcacccttt gcacctcact tctgcttacc aacgttgcgg
     8881 cacacgccaa agaagtcaaa ataggtatgg cgattgatga tctccgtctt gaacgctggc
     8941 aaaaagatcg agatattttt gtgaaaaagg cagaatctct cggcgcgaaa gtatttgtac
     9001 agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat tgaaaacatg attaaccggg
     9061 gcgtcgatgt tcttgtcatt attccgtata acggtcaggt attaagtaac gttgtaaaag
     9121 aagccaaaca agaaggtatt aaagtattag cttacgaccg tatgattaac gatgcggata
     9181 tcgattttta tatttctttc gataacgaaa aagtcggtga actgcaggca aaagccctgg
     9241 tcgatattgt tccgcaaggt aattacttcc tgatgggcgg ctcgccggta gataacaacg
     9301 ccaagctgtt ccgcgccggg caaatgaaag tgttaaaacc ttacgttgat tccggaaaaa
     9361 ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc ggaaaacgca ttgaaaatta
     9421 tggaaaacgc gctaaccgcc aataataaca aaattgatgc tgtagttgcc tcaaacgatg
     9481 ccaccgcagg tggggcaatt caggcattaa gtgcgcaagg tttatcaggg aaagtagcaa
     9541 ttttcggcca ggatgcggat ctcgcaggta ttaaacgtat tgctgccggt acgcaaacta
     9601 tgacggtgta taaacctatt acattgttgg caaatactgc cgcagaaatt gccgttgagt
     9661 tgggcaatgg tcaggaacca aaagcagata ccactctgaa taatggtctg aaagatgttc
     9721 cctcccgcct gctgacaccg atcgatgtga ataaaaacaa catcaaagaa acggtagtta
     9781 aagacggatt ccacaaagag agcgagctgt aagcgttacg ccccggcgag gagcgggggc
     9841 gtgatttctc tccatgccgc gtgaatgaat tggcttaggt ggagtcgtta tgccttatct
     9901 acttgaaatg aagaacatta ccaaaacctt cggcagtgtg aaggcgattg ataacgtcag
     9961 cttgcggttg aatgctggcg aaatcgtctc actctgtggg gaaaatgggt ctggtaaatc
    10021 aacgctgatg aaagtgctgt gtggtattta tccccatggc tcctacgaag gcgaaattat
    10081 ttttgcggga gaagagattc aggcgagtca catccgcgat accgaacgca aaggtatcgc
    10141 catcattcac caggaattgg ccctggtgaa agaattgact gtgctggaaa atatcttcct
    10201 gggtaacgaa ataacccaca atggcattat ggattatgac ctgatgacac tacgctgtca
    10261 gaagctgctc gtacaggtca gtttatctat ttcaccagat acccgcgttg gcgatttagg
    10321 gcttgggcaa caacaactgg ttgagattgc caaggcactt aataaacagg tgcgtttgtt
    10381 aattctcgat gagccgacag cctcattaac tgagcaggaa acgtcggttt tactggatat
    10441 tattcgcgat ctacaacagc acggtatcgc ctgtatttat atttcgcata aactcaacga
    10501 agtcaaagcg atttccgata cgatttgcgt tattcgcgac ggtcagcaca ttggtacgcg
    10561 tgatgctgcc ggaatgagtg aagacgatat tatcaccatg atggtcgggc gagagttaac
    10621 cgcgctttac ccaaatgaac cacataccac cggagatgaa atattacgta ttgaacatct
    10681 gacggcatgg catccggtta atcgtcatat taaacgagtt aatgatgtct cgttttccct
    10741 gaaacgtggc gaaatactgg gtattgccgg actcgttggt gccggacgta ccgagaccat
    10801 tcagtgcctg tttggcgtgt ggcccggaca atgggaagga aaaatttata ttgatggcaa
    10861 acaggtagat attcgtaact gtcagcaagc catcgcccag gggattgcga tggtacccga
    10921 agacagaaag cgcgacggca tcgttccggt aatggcggtt ggtaaaaata ttactctcgc
    10981 cgcactcaat aaatttaccg gtggcattag ccagcttgat gacgctgcag agcaaaaatg
    11041 tattctggaa tcaatccagc aactcaaagt taaaacgtcg tcccccgacc ttgctattgg
    11101 acgtttgagc ggcggcaatc agcaaaaagc gatcctcgct cgctgtctgt tacttaaccc
    11161 gcgcatcctc attcttgatg aacctaccag gggtatcgat attggcgcga aatacgagat
    11221 ctacaaatta attaaccaac tcgtccagca gggtattgcc gttattgtca tctcttccga
    11281 attacctgaa gtgctcggcc ttagcgatcg tgtactggtg atgcatgaag ggaaactaaa
    11341 agccaacctg ataaatcata acctgactca ggagcaggtg atggaagccg cattgaggag
    11401 cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt agccgtaccg acatccggtg
    11461 gcttttccgg gctgaaatca ctgaatttgc aggtcttcgt gatgattgca gctatcatcg
    11521 caatcatgct gttctttacc tggaccaccg atggtgccta cttaagcgcc cgtaacgtct
    11581 ccaacctgtt acgccaaacc gcgattaccg gcatcctcgc ggtaggaatg gtgttcgtca
    11641 taatttctgc tgaaatcgac ctttccgtcg gctcaatgat ggggctgtta ggtggcgtcg
    11701 cggcgatttg tgacgtctgg ctaggctggc ctttgccact taccatcatt gtgactttgg
    11761 ttctgggact gcttctcggt gcctggaacg gatggtgggt cgcgtaccgc aaagtccctt
    11821 catttattgt caccctcgcg ggcatgttgg catttcgcgg catactcatt ggcatcacca
    11881 acggcacgac tgtttccccc accagcgccg cgatgtcaca aatcgggcaa agctatctac
    11941 ccgccagcac cggcttcatc attggcgcgc ttggcttaat ggcttttgtt ggctggcaat
    12001 ggcgcggaag aatgcgccgt caggctttgg gtttgcagtc tccggcttct accgcagtag
    12061 tcggtcgcca ggctttaacc gctatcatcg tattaggcgc aatctggctg ttgaatgatt
    12121 accgtggcgt tcccactcct gttctgctgc tgacgttgct gttactcggc ggaatgttta
    12181 tggcaacgcg gacggcattt ggacgacgca tttatgccat cggcggcaat ctggaagcag
    12241 cacggctctc cgggattaac gttgaacgca ccaaacttgc cgtgttcgct attaacggat
    12301 taatggtagc catcgccgga ttaatcctca gttctcgact tggcgctggt tcaccttctg
    12361 cgggaaatat cgccgagctg gacgcaattg cagcatgcgt gattggcggc accagtctgg
    12421 caggcggcgt cgggagcgtg gccggagcgg taatgggggc attcatcatg gcttcactgg
    12481 ataacggcat gagtatgatg gatgtaccga ccttctggca gtatatcgtt aaaggtgcga
    12541 ttctgttgct ggcagtatgg atggactccg caaccaaacg ccgttcttga ttttgataaa
    12601 aattttctca aagccggtaa cgtattaccg gttttgagtt tttgcatgat tcagcaggaa
    12661 aagaaccatg tttactaaac gtcaccgcat cacattactg ttcaatgcca ataaagccta
    12721 tgaccggcag gtagtagaag gcgtagggga atatttacag gcgtcacaat cggaatggga
    12781 tattttcatt gaagaagatt tccgcgcccg cattgataaa atcaaggact ggttaggaga
    12841 tggcgtcatt gccgacttcg acgacaaaca aatcgagcaa gcgctggctg atgtcgacgt
    12901 ccccattgtt ggggttggtg gctcgtatca ccttgccgaa agttacccac ccgttcatta
    12961 cattgccacc gataactatg cgctggttga aagcgcattt ttgcatttaa aagagaaagg
    13021 cgttaaccgc tttgcttttt atggtcttcc ggaatcaagc ggtaaacgtt gggccactga
    13081 acgcgaatat gcatttcgtc agcttgtcgc cgaagaaaag tatcgcggag tggtttatca
    13141 ggggttagaa accgcgccag agaactggca acacgcgcaa aatcggctgg cagactggct
    13201 acaaacgctg ccaccgcaaa ccgggattat tgccgttact gacgcccggg cgcggcatat
    13261 tctgcaagta tgtgaacatc tacatattcc cgtaccggaa aaattatgcg tgattggcat
    13321 cgataacgaa gaactgaccc gctatctgtc gcgtgtcgcc ctttcttcgg tcgctcaggg
    13381 cgcgcggcaa atgggctatc aggcggcaaa actgttgcat cgattattag ataaagaaga
    13441 aatgccgcta cagcggattt tggtcccgcc agttcgcgtc attgaacggc gctcaacaga
    13501 ttaccgctcg ctgaccgatc ccgccgttat tcaggccatg cattacattc gtaatcacgc
    13561 ctgtaaaggg attaaagtgg atcaggtact ggatgcggtg ggcatatcac gctccaatct
    13621 tgagaagcgt tttaaagaag aggtgggtga aaccatccat gccatgattc atgccgagaa
    13681 gctggagaaa gcgcgcagtc tgctgatttc aaccaccttg tcgatcaatg agatatcgca
    13741 aatgtgcggt tatccatcgc tgcaatattt ctactctgtt tttaaaaaag catatgacac
    13801 gacgccaaaa gagtatcgcg atgtaaatag cgaggtcatg ttgtagcgat gatgagaatt
    13861 gtcggcgtcg catcaggtaa ctcagcacaa tcacctgatg ggagcaaaag ccttatccga
    13921 ccgacaagtc ggataaggct cgaggaaatg caattacata tgtgccgcga ttaaccgttg
    13981 gttatcctgg tacattgcga acaggtagtt gttataactc ttccctttgg tcgagtagcc
    14041 cttcagcttg tgaatcatcg ctgtggcagt cacttcctga tctgctttac gcagctgtgc
    14101 acgcgattta cggaacgaag agtaagccgg atgcgtattc aggttagtaa cataggcgct
    14161 caccgattct ttgacagaac taaactgtga gtaccccttc actttacccg gcgcattagt
    14221 acaacgtcct ttcatgcatt tcatgccgaa cagattgttg ttgttgcgcg ccagcttcga
    14281 cgttccccaa ccgctttctg ctgcagccat cgtcgctacc atactggtgg ggataatgtc
    14341 tacgcgttca agcaaggtat tccacgggat ttttcgcgta ttaccagacc acttcacctt
    14401 gtagcgtttg gcgatgtctt tcagacgcgc acgctcagca ggtgaccatt ggccctgata
    14461 ctgttttgaa atcagccagt tacgttccgc agtaatggcc gcattttggc tggtaatgta
    14521 aggcatcaca gtccggagaa acgccttttt ccttggtgtt ccggaagggt attttcgcaa
    14581 atcaggaagt gaactactct ttgcactatt gcgagaatac tcttgtttac tgcttacctg
    14641 tttattacta gctttagtta agtgggactt ttgactcgct gttgttgtgt gcgtcttcgc
    14701 taacacctca ctcgaaaaca ccagagtgag taacataaga atcatcgccc catatcgtcg
    14761 tatgggagtc aaaatcatca ggtctcctgg tcggatttaa tcattccaac accttatatt
    14821 tttcacaaat ttgagagttg aatctcaaat catatcaaaa atagctgtca agagcacccc
    14881 aaggaatagt ccaaatctga aactatgtca cgagttaacg attcagattg gcgctaaatc
    14941 gcagaaaatg tggggtttat cgcaaaattc agccgttttt tgcgcgcgat cgctcaccct
    15001 tgcttctcat cctgtggact taccgctcag ggatgagttt tgtctggctt atcgctggca
    15061 aactgtctga aatcgcagca ataaggactc attcaccatg aaactcgccg cctgttttct
    15121 gacgctcctt cctggcttcg ccgttgccgc cagctggact tctccggggt ttcccgcctt
    15181 tagcgaacag gggacgggaa catttgtcag ccacgcacag ttgcccaaag gtacgcgtcc
    15241 actaacgcta aattttgacc aacagtgctg gcagcctgcg gatgcgataa aactcaatca
    15301 gatgctttcc ctgcaacctt gtagcaacac gccgcctcaa tggcgattgt tcagggacgg
    15361 cgaatatacg ctgcaactag acacccgctc cggtacacca acattgatga tttccatcca
    15421 gaacgccgtc gaaccggtag caagcctggt ccgtgaatgc ccgaaatggg atggattacc
    15481 gctcacactg gatgtcagcg ccactttccc ggaaggagcc gccgtacggg attattacag
    15541 ccagcaaatt gcgatagtga agaacggtca aataacgtta caacccgctg ccaccagcaa
    15601 cggtttactc ctgctggaac gggcagaaac tgacgcccct gccccttttg actggcataa
    15661 cgccacagtt tactttgtgc tgacagatcg tttcgaaaac ggcgatccca gtaatgacca
    15721 gagttacgga cgtcataaag acggtatggc agaaattggc acttttcacg gcggcgattt
    15781 acgcggcctg accaacaaac tggattacct ccagcagttg ggcgttaatg ctttatggat
    15841 aagcgcccca tttgagcaaa ttcacggctg ggtcggcggc ggtacaaaag gtgatttccc
    15901 gcattatgcc taccacggtt attacacaca ggactggacg aatcttgatg ccaatatggg
    15961 cagtgaagcc gatctacgga cgctggttga tagcgcgcat cagcgcggta ttcgtattct
    16021 ctttgatgtc gtgatgaacc acaccggcta tgccacgctg gcggatatgc aggagtatca
    16081 gtttggcgcg ttatatcttt ctggtgacga gctgaaaaaa acgctgggtg aacgctggag
    16141 tgactggaaa cctgccgccg ggcaaacctg gcatagcttt aacgattaca ttaatttcag
    16201 cgacaaaaca ggctgggata aatggtgggg aaaaaactgg atcagaaccg atatcggcga
    16261 ttacgacaat cctggatttg acgatctcac tatgtcgctg gcctttttgc cggatatcaa
    16321 aaccgaatca acgaccgctt ctggtctgcc ggtgttctat aaaaacaaaa cggataccca
    16381 cgctaaagtc atcgacggct ttacccctcg cgattactta acccactggt taagtcagtg
    16441 ggtccgcgac tatgggattg atggttttcg ggtcgacacc gccaaacatg ttgagttgcc
    16501 cgcctggcag caactgaaaa ccgaagccag cgccgcgctt cgcgaatgga aaaaagcgaa
    16561 ccccgataaa gcgttagatg acaaaccttt ctggatgacc ggtgaagcct ggggccacgg
    16621 cgtgatgcaa agtgactact atcgccacgg cttcgatgcg atgatcaatt tcgattatca
    16681 ggagcaggcg gctaaagcag tcgattgtct ggcgcagatg gatacgacct ggcagcaaat
    16741 ggcggagaaa ttgcaggatt tcaacgtgtt gagctacctc tcgtcgcatg atacccgcct
    16801 gttccgtgaa gggggcgaca aagcagcaga gttattatta ttagcgccag gcgcggtaca
    16861 aatcttttat ggtgatgaat cctcgcgtcc gttcggcccc accggttcgg atccgctgca
    16921 aggtacgcgt tcggatatga actggcagga tgttagcggt aaatctgccg ccagcgtcgc
    16981 gcactggcag aaaatcagcc agttccgcgc ccgccatccc gcaattggcg agggcaaaca
    17041 aacgacactt tcaatgaagc agggctacgg ctttgttcgt gagcatggcg acgataaagt
    17101 gctggtcatc tgggctgggc aacagtgact tttccggctt cccgttcgtc agtacctcgg
    17161 gaagccgcca accaggataa aatgtcagct ctaatcagcg ttgcaggata aagcaccgct
    17221 ccctcttcaa cagagcgatt tgcaccccag caaatgtagc gttattgtta ccttccttgc
    17281 tacagagttc gacagatatc ccgctatgac attctccctt tttggtgaca aatttacccg
    17341 ccactccggc attacgctgc tgatggaaga tctgaacgac ggtttacgca cgcctggcgc
    17401 gattatgctc ggcggcggta atccagcgca gatcccggaa atgcaggact acttccagac
    17461 gctactgacc gacatgctgg aaagtggcaa agcgactgat gcactgtgca attacgacgg
    17521 tccacagggg aaaacggagc tactcacact gcttgccgga atgctgcgcg agaagttggg
    17581 ctgggatatc gaaccacaga atattgcact aacaaacggc agccagagcg cgtttttcta
    17641 cttatttaat ctgttcgccg gacgccgtgc cgatggtcgg gtcaaaaaag tgctgtttcc
    17701 gctggcaccg gaatacattg gctacgctga cgccggactg gaagaagatc tgtttgtctc
    17761 tgcgcgtccg aatattgaac tactgccgga aggccagttt aaataccacg tcgattttga
    17821 gcatctgcat attggcgaag aaaccgggat gatttgcgtc tcccggccga cgaatccaac
    17881 aggcaatgtg attactgacg aagagttgct gaagcttgac gcgctggcga atcaacacgg
    17941 cattccgctg gtgattgata acgcttatgg cgtcccgttc ccgggtatca tcttcagcga
    18001 agcgcgcccg ctgtggaatc cgaatatcgt actgtgcatg agtctttcca agctggggct
    18061 accaggctcc cgctgcggca ttatcatcgc caatgaaaaa atcatcaccg ccatcaccaa
    18121 tatgaacggc attatcagcc tggcacctgg cggtattggt ccggcgatga tgtgtgaaat
    18181 gattaagcgt aacgatctgc tgcgcctgtc tgaaacagtc atcaaaccgt tttactacca
    18241 gcgtgttcag gaaactatcg ctatcattcg ccgctattta ccggaagatc gctgcctgat
    18301 tcataaaccg gaaggagcca ttttcctctg gttatggttt aaggatttgc caattacgac
    18361 cgagcagctc taccagcgcc tgaaagcacg gggcgtgctg atggtgccgg ggcacaactt
    18421 cttcccaggg ctggataaac cgtggccaca tacacatcaa tgtatgcgca tgaactacgt
    18481 gccggagccg gagaaaattg aggcgggagt gaagattctg gcagaagaga tagaaagagc
    18541 ctgggctgaa agtcactaaa aattgcctga tgcgctacgc ttatcaggcc tacatgattt
    18601 ctgcaatata ttgaatttgg tagaatttgt aggccggata aggcgtttta gccgcatccg
    18661 gcataaacaa agcgcactat cccggcgttg agaaacgccg gaaaaacgtt tcaaacaggc
    18721 tgctgccgta gccgattccg ctgcactttc tcgacatcca tgcactgcaa cgcctttgtc
    18781 gggcaggctt caacacaggc cggtcccgtc gcccgatgcc agcacagatc gcacttaatc
    18841 gccctcgcct ttttgcgcga agaaacgacc tccatcgcac caaacgggca agccagcata
    18901 cagcttttac agccaatgca acgtgattgt tcaacgaaaa tatgcccatg ttcgcggctt
    18961 atcgcgtcaa cagggcagac attcgcgcac ggtgcatctt cacactgatg gcaggctaca
    19021 gccgtggtcc atgtgtggtc tttaatgaca cgaatacggg aaataaactc gtctggtgac
    19081 aacgcagcgc aatcctgatt ctcctgatgc gacactgcgc aagccacttc acaggtacgg
    19141 caaccgatac atttcgtcgc atccgcaata ataaaccggt tcatgatcac tcctttgcac
    19201 taaaacagca gagtgccaga gagcgcgtct ctactgcttt gatccaacga aggaaatgtt
    19261 aaagttttgg cacttgtcca aagcgttatg tgatcgcgcc ctggtcgtcc ctgacagtaa
    19321 atcccagttc attagaaata gcctgcgcgg tttcacgcag tggtttcagg agatttttct
    19381 cccccacctg tttcagacgt gatgtcgaaa gagaaatcga cacggcgtac ggcacccgcc
    19441 catgaatatc aaacaccgga acagcaatac aagagacgcc gagttcgttt tcttctctgt
    19501 ccatcgccgc tccgctttca cgaatgtgcg ccagttcgtc gaacatcgcg ggcagctcgg
    19561 taatggtatt gcgggttaac ggctggatct catgctgatg gctttcccag tatgacttca
    19621 cgtagtccgg gtgaccaaac gccatgtaga tcttgcccat tgcggaacag tagagcggca
    19681 tatgctggcc tatataggca cgggttcgca gcatcccggt tgtgggttcc agcttataaa
    19741 tcaaaatagc gtgatcgtct tcgcggctgg agaagttaat ggtttcacca gtggcgatgt
    19801 tcagtgcctc aagatgcgga gcggcgatat gaatgatatt cagcgaagac agcgccttct
    19861 gcccgacggc aataaatttg gtagtcaggc ggtaactccc tgcggcgggc gcggtggtca
    19921 catagccgca ggactgtaat ccctgcaata agcgatggac ggtactctta tttaaaccag
    19981 ccagttccga aagatgcgcc aacggacaac cgtttggata gttgctcaaa atctcaatca
    20041 gcatcaaccc gcgaaacaga ctctggcttc cggctggacg ctctttttcc tgcgccatct
    20101 cgttctcttt ttttcccatc acttctttcc ccattttgtc gcgtcctgat ggtagcgcaa
    20161 agtgtgtcgt agttcacgat ctcgacagat aatttataac cacttgattt ttaagttttt
    20221 tgaaattaat caatcagatt gctttgttaa aaagcgatcg atatatttga aatcagattt
    20281 cgcatattga aattttaagc caaaaaagcg atcaaaaaaa acaaggaagc cagggatgaa
    20341 agtgacattt gagcagttaa aagcagcctt taatcgagtc ttaatttcac gcggcgttga
    20401 cagcgaaacg gctgatgcct gtgccgagat gttcgcccgc accaccgaat ccggcgttta
    20461 ttctcacggc gtcaaccgtt tccctcgttt cattcaacaa ctggaaaacg gcgatatcat
    20521 tcctgatgcc caacccaaac gtataaccag cctcggcgca attgaacagt gggacgccca
    20581 gcgttcgatc ggtaacctga cagcgaaaaa gatgatggat cgcgccatcg aactggctgc
    20641 cgatcacggt attggtctgg tggctctacg taatgccaac cactggatgc gcggcggcag
    20701 ctacggctgg caggcggcgg aaaaaggcta tattggcatt tgctggacca actccatcgc
    20761 cgtaatgccg ccgtggggcg caaaagagtg tcgcatcggc accaacccgc tgatcgtcgc
    20821 cattccttct accccaatca ccatggtcga tatgtcgatg tcgatgttct cttacggcat
    20881 gttagaagtt aaccgtctgg caggccgtca gctcccggtc gatggtggct ttgatgatga
    20941 gggcaatttg accaaagaac ctggcgttat cgagaagaat cgccgcattt tgccgatggg
    21001 ctactggaaa ggttctggca tgtcgattgt gctggatatg atcgccactc tcctttccga
    21061 cggcgcatcg gttgccgaag tcacccagga caacagcgac gaatacggcg tttcgcaaat
    21121 ctttatcgcc atcgaagtgg ataaattgat cgacggcccc acccgcgatg ccaagctgca
    21181 acgcatcatg gattacgtta ctaccgctga acgcgctgac gaaaaccagg ccatccgctt
    21241 acccggccac gaatttacta ccctgctggc cgaaaaccgc cgtaacggca tcaccgttga
    21301 tgacagcgtg tgggccaaaa tccaggcgtt atgaggagat aagtcatgat ttttggacat
    21361 atcgcgcagc ctgacccgtg ccgtttgcct gccgccattg aaaaggggct cgattttctg
    21421 cgcgctaccg atttcaacgc cctggaaccg ggcgttgtcg aaatcgacgg caagaatatt
    21481 tatgcgcaaa ttatcgattt aaccacacgc gaagcggtgg aaaaccgtcc ggaagtgcat
    21541 tgtgggtata tcgatattca gtttctggcg tggggtgaag agaaaattgg cattgccatt
    21601 gatacgggaa acaataaagt cagcgaatca ctattagagc agcgcgatat tattttttat
    21661 cacgatagtg aacatgaatc atttatagaa atgataccgg gcagctacgt tattttcttc
    21721 ccgcaggatg ttcatcgacc aggttgtatt ctgcaaacag cctctgagat acgaaaaatt
    21781 gtggttaaag tcgcattaac agcgctgaat taatcgaata atcgtctacg ccagaacgcc
    21841 tgggtaatgt tatcgctcta actatattaa taatttaggt tgctgcacat gcaacttgaa
    21901 ttatgacaaa catagcctca aggaatagct atgaaaaaaa tacttgaagc aatactggcg
    21961 attaatctcg ccgtactttc ctgtattgtg tttataaaca ttattttaag atatggtttt
    22021 cagacaagca ttttatctgt agatgaattg tcacgttatt tatttgtctg gttaacattt
    22081 attggcgcga ttgtggcttt tatggataac gcccacgttc aggtcacctt tctggtggag
    22141 aaactctccc ccgcatggca gcgacgagtc gctctggtta cccattcctt aatcttattt
    22201 atttgtggcg cactggcctg gggcgcaacg ctaaaaacaa ttcaggactg gagcgattat
    22261 tcaccgatcc tcggtttgcc catcggcctg atgtatgccg cttgtctgcc caccagcctc
    22321 gttatcgcat tctttgaact gcgtcattta tatcaactca tcacgcgtag caattctcta
    22381 acgtcgccac cgcaaggagc ttaatcatgg ctgtgctgat ttttctgggc tgtctgttgg
    22441 gtggtattgc tatcggtttg cctattgcct gggcactgtt gttgtgcggg gcggcgttaa
    22501 tgttctggct ggagatgttt gatgtccaga tcatggcaca aacgctggtg aacggagccg
    22561 acagcttctc cctgctggcg attccgttct ttgtgctggc gggtgaaatc atgaatgcgg
    22621 gcggcttgtc aaaacgcatt gttgacctgc cgatgaaact ggtggggcat aaaccaggcg
    22681 ggctgggcta cgtcggcgtg ctggcggcta tgattatggc aagcctttct ggttctgccg
    22741 ttgcggatac cgccgccgtt gccgctctac tggtgccgat gatgcgcagc gccaactacc
    22801 cggtcaaccg ggcggcgggg ctgattgcct ctggcggcat tatcgcgcca attattcccc
    22861 cttccattcc gtttattatt ttcggcgttt ccagcggatt atccatcagt aagctgttta
    22921 tggcgggcat tgcccctggc atgatgatgg gcgcaacgct gatgcttacc tggtggtggc
    22981 aggccagtcg ccttaattta ccccgccagc aaaaagcaac gatgcaggaa atctggcact
    23041 cttttgtctc cggtatctgg gcgctgtttc ttccagtcat cattattggc gggtttcgct
    23101 ccggtctgtt tacccctacc gaagccgggg ccgttgctgc tttctatgcg ctgtttgtcg
    23161 ccacagttat ttaccgtgaa atgaccttcg ccacactttg gcatgtgctc attggcgcag
    23221 cgaaaacgac ctcagtggtg atgtttctgg tggcctcggc acaagtttcc gcctggctga
    23281 tcaccattgc tgaactgccg atgatggttt ctgatttact gcaaccgctg gtcgattcac
    23341 cgcgtctgct gtttatcgtc attatggtgg ctatcctgat tgtcggcatg gtcatggatt
    23401 taacgccgac ggtattaatt cttaccccgg tattaatgcc tttagttaaa gaggcaggaa
    23461 tcgatccgat ttatttcggt gtcatgttta tcattaactg ctcaatcggc ttaattacac
    23521 cgcctatcgg taatgtgctt aacgttattt caggggtggc aaaactcaaa ttcgatgatg
    23581 cagtcagagg cgttttccct tacgtcctgg ttttatattc attattagtc gtatttgttt
    23641 ttattcccga tctcatcatc ctccctttga aatggattaa ttaaaaggaa aatattatga
    23701 aattacgctc tgtaacctac gcattattca ttgctggcct ggctgcattc agtacatctt
    23761 cactggcggc acaatcttta cgtttcggtt atgaaacctc acaaaccgac tcgcaacata
    23821 ttgcggcgaa aaaattcaat gatttattgc aggagagaac caaaggcgag ctgaaattaa
    23881 aactgttccc ggacagcact ctcggtaacg cgcaggcgat gatcagcggc gtacgtggcg
    23941 gcaccatcga tatggaaatg tccggctcga ataactttgc cgggttatca ccagtgatga
    24001 acttgcttga tgtccctttc ctgttccgcg ataccgctca cgcgcataaa acgctcgacg
    24061 gcaaagtcgg tgatgatctg aaagcctccc ttgaaggtaa aggactaaaa gtactggcct
    24121 actgggaaaa cggctggcgc gatgtcacca actcgcgcgc accggttaaa acccccgccg
    24181 acctgaaagg gctgaaaatt cgcactaaca atagcccgat gaatatcgcc gcattcaaag
    24241 tctttggcgc taacccgatc ccgatgccgt ttgccgaagt ctataccggg ctggaaacgc
    24301 gcactatcga cgctcaggaa cacccgatca acgtcgtctg gtcagcaaaa tttttcgaag
    24361 tacagaagta cctttctctg acgcaccacg cctattcgcc acttctggtg gtgatcaaca
    24421 aagcgaagtt tgatggctta agcccggagt tccagcaggc gctagtttca tctgcacaag
    24481 aagcgggtaa ctatcagcgc aaactggttg ctgaagatca gcaaaaaatc atcgatggca
    24541 tgaaagaagc gggcgtggaa gtcatcaccg ttctcgaccg caaagccttt agcgacgcac
    24601 tgggtaatca ggtccgcgac atgtttgtta aagatgttcc gcagggtgct gatctgctga
    24661 aagctgtgga tgaggtgcaa taaaccatga cgcaatactg gctggggtta gattgtggcg
    24721 gtagctggct gaaagccggg ctgtatgacc gcgaaggccg ggaggcaggc gtgcagcgcc
    24781 tgcctctgtg cgcattaagc ccgcagccag gctgggcaga gcgcgatatg gcagaacagt
    24841 ggcaatgctg catggctgtc attcgcgccc tgcttaccca ttccggcgtt agcggggaac
    24901 aaatagtggg tatcggtatc tccgcacagg gaaagggctt gtttttgctg gataaaaaca
    24961 acaagccgct cggtaacgcc attttgtcct cagaccgccg ggcgatggaa atcgttcgtc
    25021 gctggcagga agatggcatc ccggaaaaac tctacccgct gacccgacaa accttgtgga
    25081 ccgggcatcc ggtgtcgctg ttacgctggc tgaaagagca cgaaccagaa cgctacgcgc
    25141 aaattggctg cgtgatgatg acgcacgact acctgcgctg gtgtttaacc ggcgtcaaag
    25201 gctgcgaaga gagcaatatt tccgagtcca atctctacaa catgagtctt ggggaatatg
    25261 acccgtgcct caccgactgg cttgggatcg ctgaaatcaa ccacgccctg ccgcctgttg
    25321 tcggatctgc cgaaatctgc ggtgagatca ccgctcagac agccgtactg accggtctga
    25381 aagcgggtac gcccgttgtc ggcggcctgt ttgatgtggt ttccaccgca ctctgcgccg
    25441 ggatcgaaga cgaatttacc ctcaatgcgg taatgggcac ctgggcggtg accagcggca
    25501 taacctgcgg tttgcgtgac ggtgaagcgc atccgtatgt ctatggtcgc tacgttaacg
    25561 acggtgaatt tatcgttcat gaagccagcc ccacctcttc cggcaacctc gaatggttta
    25621 ccgcacagtg gggagaaatc tcgtttgatg agatcaacca ggccgttgcc agcttgccga
    25681 aagccggtgg cgatctcttt ttcctgccgt tcctgtacgg cagcaacgcc ggactcgaga
    25741 tgaccagcgg tttctacggg atgcaggcca ttcacacccg cgcacacctg ttgcaggcca
    25801 tttatgaagg cgtagtgttc agccatatga cccatctcaa ccggatgcgc gaacgtttta
    25861 ctgatgtgca caccctgcgc gtcactggcg gcccggcgca ttccgatgtc tggatgcaaa
    25921 tgctggcgga cgtcagcggg ctgcgtatcg aactgccgca ggtggaagaa accggctgct
    25981 ttggtgcggc acttgccgcc cgcgtcggca ccggagttta tcgcgatttc agcgaagccc
    26041 aaggtgattt acagcacccg gtacgcaccc tgctgccgga tatgaccgcc catcagcttt
    26101 accaacaaaa ataccaacgc tatcagcatc tcattgccgc acttcagggc tttcacgccc
    26161 gcatcaagga gcacacatta tgagccgacc acttctgcaa ctggccctcg accactcatc
    26221 acttgaagac gcacagcgcg acgtgatgca gttaaaagac agcgtcgata tcgtcgaagc
    26281 gggcaccatt ctctgtttaa acgaagggct tggcgcggta aaagccttgc gcgaacagtg
    26341 cccgaacaaa ctcatcgtcg ctgactggaa ggtcgccgat gccggtgaaa cgctcgctca
    26401 acaagcattt accgcaggtg ctaactggat gaccatcatc tgcgccgcac cgctcgcgac
    26461 ggtggaaaaa ggccacgcga tggcgcaacg ctgcggcggt gaaattcaga tagagctgtt
    26521 cggtaactgg acgctggacg acgcccgcga ctggcatcgt attggcgtgc ggcaggcgat
    26581 ttatcatcgc ggtcgcgacg cgcaagccag cgggcaacag tggggcgaag ccgatcttgc
    26641 acgcatgaag gcgctttccg atatcggcct tgagctttcc attaccggcg gtattactcc
    26701 tgctgatctg ccactgttta aagatatccg cgtaaaggcg tttattgccg gacgggcgct
    26761 ggcaggcgca gcaaatccgg tgcaggtggc tggcgatttc catgcgcaaa tcgacgctat
    26821 ctggggaggc aagcgtgcgt aatcatccgt tagggattta tgaaaaagcg ctggcgaaag
    26881 atctctcctg gccggagcgg ctggtgctgg caaaaagctg tggctttgat tttgtcgaaa
    26941 tgtcggtgga tgaaaccgac gaacggctct cacgtcttga ctggagcgcc gcacaacgtg
    27001 cttcgctggt cactgcgatg atcgaaacgg gtgttgccat tccgtcaatg tgcctgtctg
    27061 cccatcgtcg ttttcccttt ggcagccggg acgaggcagt gcgcgaacgg gcgcgggaaa
    27121 tcatgagtaa agcgattcgc ctggcgcgcg atctcggcat tcgcaccatt cagctggcag
    27181 gttatgacgt ctattacgaa gaccacgacg aaggcaccca acaacgtttt gctgaagggc
    27241 tggcgtgggc agtcgaacag gcggcggcat cacaagtcat gctggcggtg gagattatgg
    27301 ataccgcgtt tatgaactcc atcagcaaat ggaaaaaatg ggacgagatg ctcgcctcgc
    27361 cgtggttcac cgtctacccg gacgtcggca acctcagcgc ctggggcaat gatgttcccg
    27421 ccgaactgaa actgggcatt gaccgtatcg ccgcgatcca cctgaaagac acccagccag
    27481 tgaccgagca aagccccgga cagttccgtg atgtgccgtt tggcgaaggc tgcgtcgatt
    27541 tcgttggcat cttcaaaacg ctgcatgaac tgaactatcg cggatcattt ctgattgaga
    27601 tgtggaccga aaaagccaaa gagccggtgc tggagattat tcaggcgcgg cgctggattg
    27661 aagcgcgtat gcaggaggcc ggatttatat gttagagcaa ctaaaagccg acgtgctggc
    27721 ggcgaatctg gcgcttcccg ctcaccatct ggtgacgttc acctggggca atgtcagcgc
    27781 ggtagacgac acgcggcaat ggatggtaat caaaccttcc ggcgtcgagt acaacgtgat
    27841 gaccgccgac gatatggtag tggttgagat agccaccggt aaggtggtgg aaggcagaaa
    27901 aaaaccctcg tccgacacgc caacgcatct ggcgctttac cgtcgctatg ccgaaattgg
    27961 cggtattgtg catacccact cgcgccacgc caccatctgg tcgcaggccg ggctggatct
    28021 ccccgcctgg ggcaccacgc acgctgatta cttctatggt gccatcccct gcacgcgaca
    28081 gatgaccgca gaggagatca acggcgagta tgaataccag accggcgaag tgatcattaa
    28141 aaccttcgaa gaacgtgggt tgaacccggc acaaatcccg gctgtactgg tgcattctca
    28201 cggcccgttc gcctggggaa aaaacgccgc cgatgcagtg cataacgccg tggtgctgga
    28261 agagtgcgcc tatatgggcc tgttctcgcg ccagcttgca ccgcagctcc ctgcgatgca
    28321 aaacgaactg ctggataagc actacctgcg taaacatggg gccaatgcct attacgggca
    28381 gtaatccctc acgccggggc ttcattgccc cggcactacg aattgatatg ttccttgctg
    28441 taacgtcgct tccacgcagc tggcgttaag ccggtgtgtt tgcgaaaaat ctgccggaaa
    28501 tagccgacgt cattaaaccc gcacaacgcc gccacttccg tgagtgatcg cgcttcgcta
    28561 attaacagct tttcggcttc gcgcacccgc tggcggtgga ttgcttctgt taacgtcaga
    28621 tgaaacaccc gccgatagac gcgcccaaga taatcggcat tgcagtacag ctcgcgggct
    28681 aaaagcgccg tggaaagcgg gcggtgatat tgcgtgcgaa tcagtttctg cgctttccag
    28741 gcaagggtgg tgcctgcacc atcggcctgt tcatcaagac gcgcgtcacg ggtgagctgc
    28801 tgcaaaatca gcagcacaat aaactccagc gccggagtgc gttgatgctt ttcctgttcg
    28861 cttaagaact ggcgaaacag ggccaccagc acctgcggat cggcaacctt agtttgttgc
    28921 ggcacgctaa gatgagtgag ccagcgcgga tcgttgtccg gcgctttaac ctcaaaatgc
    28981 agccagtaaa atttgagatc ggcgggaaaa tcctctaccc caacatgctg tcggttcggc
    29041 cagagtaata aactctctcc cgcatcgacc agaaatatcc gctcttcttc ctgaatcgct
    29101 aatcgacctt tttcgacaaa gataatttcc catgactcca gccgccttgc cggatgcctc
    29161 cctacgccac gggaaataaa taatccacca ttttgcactt taatcggtaa cgtcatggat
    29221 aattcgagca ttgatatgta atttcccgct tattcattaa tgattaaaca cattactttt
    29281 tgattattaa tcaaaaacat taatttcacc tgatccaatt cacactttta aattcaggtc
    29341 ggaatcgtca ccttttttta ctcttttctc gccttgttga tattcccgtt cacggcagaa
    29401 ttaatcaggt cattgcaata aaactaaaaa ttgctcccta ttcacgagga atatctatga
    29461 cctctacacc gattactacc gctgatatcg ctaaaggtaa aattgacgat gcgttatctg
    29521 tacgggaaaa aataggctac ggcctgggtg acgcaggcgg caccgtaata acttgcctga
    29581 tcatgaactt tctcaccttt ttctacaccg acgtttttgg cttaactccg gcgctggtcg
    29641 gcacgctgtt tattgcactg cgcgtgtttg atgccatctc cgacccggtg atgggcatca
    29701 ttgccgaccg gacgcaaagt cgctgggggc gctttcgtcc gtggcagttg tggattgcca
    29761 ttcccatcgg cattatcggc atcctgacgt tcaccgtgcc agatgccagc atgggagtaa
    29821 aaatcgcctg ggcgttcggc acttacctgc tcctttcagt cggttatacc gccatcaacg
    29881 tgccgtactg cgcgctgatc aacaccatga ccacccgcca caatgaagtg atctcctgcc
    29941 agtcctggcg attcgttctc tgcggcgtag cgggatttct ggtttcggta ggcttaccgt
    30001 ggatggtgga tctcttcggt cagggtaatg ccgcgcgcgg ctatcaactg ggcgtcggcg
    30061 tgctgtgcgc catcgctgtg gtgatgttcc tgtgctgttt cttctgggtt cgtgaacggg
    30121 taccactctc cacaatgggg aaatttaccc tgcgcgaaca tcttgccggg ctgcgaaaca
    30181 acgaccaact gctgctgatg ctggtcatgt ctttcctgct gattaacgtc tttaacattc
    30241 gcggcggtgg gtatatgtac ttcattacct acgtcttaca aggcagtacg ggctacacgt
    30301 cgctgttctt caccatggtc accttcgcct ccattatcgg ctcggtgatt gtcagcccgt
    30361 taacgcgacg tttcgatacc gtcaaaattt actactacac caacctgctc ctcgccgcgc
    30421 tggcggtgtt gatgtggttc ctgccttctg gcccggctta tcaaacgctg tggctggcgg
    30481 tgatcctcgg taatggcgtg attcttggct tcacattgcc attgcacttc tcattgatgg
    30541 cctttgccga tgactacggc gagtggaaaa cccgcgtacg ttcttccggc atgaacttcg
    30601 ccttcaatct gtttttcatc aaactggcct gggcctccag cgccgggatc atcagcctgc
    30661 tgtttatttt tgtcgcctac cagccaggcg tggaaaacca gaccaccagt tcgcttggcg
    30721 ggatcacggc gatggaaaca ttactgcctg cgctattcca cctgctgctg gcaggggcga
    30781 tccgcttttg caaactcaat aaccctatga tgtcacgcat tgctaccgac ctgcgtcagc
    30841 gtcatgtaca gccttaagga gcacaagatg aacatttcgg aagtcgatct gcataaactg
    30901 acggtcagcg atccgttcct cggtcagtac caacaactgg tccgcgatgt ggtgattcct
    30961 tatcagtggg acgccttgaa cgatcgtatc ccagaagcgg aacccagcca tgcgattgaa
    31021 aactttcgca ttgccgcagg actgcaagac ggtgaatttt acgggatggt gtttcaggac
    31081 agcgacgtcg ccaaatggct ggaagcggta gcctggtcgc tgtgccagaa gccggatgcc
    31141 gaactggaaa aaaccgctga cgaggtaatc gaactggtcg cttccgccca gtgcgaagat
    31201 ggctatctca atacttactt tacggtaaaa gcgcccgaag aacgctggag caatctggcg
    31261 gagtgtcatg aactttactg cgccggtcat ctgattgaag ccggagtcgc cttcttccag
    31321 gccacgggca agcggcgctt gctggaagtg gtttgccgtc tggccgatca tatcgacagc
    31381 gtatttggtc caggtgaaag taagttacac ggttatcctg gtcacccgga aattgaactg
    31441 gcgctaatgc gcctgtatga agtgaccgaa gagccgcgct acctggcgct gacgaactat
    31501 tttgtcgaac agcgtggtgc gcaaccgcac tattacgacc aggaatatga aaagcgcggg
    31561 cagacctcgc actggcacac ctacggcccg gcgtggatgg tgaaagataa agcctacagc
    31621 caggcgcatt tgcccatcgc acagcaacaa accgccatcg gacatgcagt acgctttgta
    31681 tatctgatga ctggcgtcgc acatctggcg cgtttaagtc acgatgaaag caagcgtcag
    31741 gattgcctgc ggctgtggaa caatatggcc cagcgtcagt tatatattac cggcggcatt
    31801 ggctcacaga gcagcggtga agcgttcagc agcgattacg atctgccgaa tgacacggta
    31861 tacgctgaaa gttgcgcctc catcggcctg atgatgtttg cccgacgaat gctggaaatg
    31921 gaaggcgaca gtcaatatgc cgatgtgatg gagcgcgcac tgtacaacac cgtgctcggc
    31981 ggcatggcac tggatggcaa acatttcttc tatgtgaatc cgctggaagt acatccaaaa
    32041 tcgctgaaat tcaaccatat ctacgatcac gttaaaccga tccgccagcg ttggtttggc
    32101 tgcgcttgtt gtccgccaaa tatcgcccgc gtactgacct cgattggtca ttatctctac
    32161 acgccgcgtg aagatgcgtt gtatatcaac atatatgcag gaaacagcat ggaagtgccg
    32221 gtagaaaatg gcacgttgcg cctgcggatt agcgggaact atccgtggca ggaacaggtg
    32281 acaattgcgg ttgaatcgcc ccagccggtg cgtcatacgc tggctttacg tctgccggac
    32341 tggtgcacac agccgcagat catattgaat ggggaagagg tcgggcagga tattcgtaaa
    32401 gggtatttgc acattacccg cgaatggcag gagggcgata cgctgaatct gactttgccg
    32461 atgccggtac gccgcgttta cggtaacccg ctggtgcgtc acgtcgccgg aaaagtggcg
    32521 attcagcgcg gcccgctggt gtattgcctg gaacaggccg acaacggcga gtcactacat
    32581 aacctgtggc tgcccgccga tgcgccattt acgacctttg aaggcaaggg attgttcagc
    32641 cataagatct taatccaggc accgggttac cggtatgaac agagcaatcc agagcagcaa
    32701 ccgctgtggc attacgacag cgcgccagcc aaacgccagc cgcaaactct gacgtttatc
    32761 ccgtggttta gctgggctaa ccggggtgaa ggcgaaatgc ggatctgggt gaatgaggaa
    32821 aagcattgcc atccgtaggt tggataaggc actcgcgccg catccaccgt agggtgcaca
    32881 ttgcctgatg cgacgcttaa aacgtcttat caggcctaca agtcggtgcg agagcgtagg
    32941 ccagccgcac cgacatccac cggtgaatta aaaacgccag ctcacgccgg tgacaaaggt
    33001 aaaactctca ttacgatcaa ccatcgggct gtcggtaatc tcttccggca gctcgctgta
    33061 tcctgcgctc gccatcaata cgacgtgctc tcctatcggg tattttgccg tcaggctgac
    33121 atagggcacc caggcatcct gcgcggaata acttgccaga ccgctacgac gggactcact
    33181 ctctgaaatg ccatagtaat agtcactgaa attctcgtca taatagagaa cgcccagtgc
    33241 aggtgtcagc gacaggcgac ctatctgcat tttgtggaat accgatagct cccccaccca
    33301 gccgttgctg ttatccagaa cgtccgcagc cgcagaggct ttcacggttc cccaacgctc
    33361 gtggtgatac caggcaaccc ccgccatagc cgtactatca cgcttatcaa gctgttgcat
    33421 ggcataatcg tcattatcgg cagggtcaaa ttccagcggc atccaggatg cggtcaggct
    33481 aagttcattt ttttcacttt gcgacagaat aaaacctaac gtggtctgac gaacataaaa
    33541 agtatcacct tcataactaa tcagcggaat tgcctttgta ttttcattat aaccgcgata
    33601 aggcgattca ttataagccg caccagcacc aatggataat tcagaagcag ttgcgcttgc
    33661 cataaaaggc aacgcaaata acgccacaat attgcggtta attaacatgt ccggtattcc
    33721 attttaaaat a
//
LOCUS       DAPOZO010000052        33614 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPOZO010000052 DAPOZO010000000
VERSION     DAPOZO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.052"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(18..93)
                     /locus_tag="ROI21_002652"
     tRNA            complement(18..93)
                     /locus_tag="ROI21_002652"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="ROI21_002653"
     tRNA            complement(152..228)
                     /locus_tag="ROI21_002653"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="ROI21_002654"
     CDS             complement(331..1716)
                     /locus_tag="ROI21_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDX5111560.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWVNYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKIFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="ROI21_002655"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="ROI21_002655"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDX5111561.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRLKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ROI21_002656"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ROI21_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDX5111562.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGVALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNTANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ROI21_002657"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ROI21_002657"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDX5111563.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQRKGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVAP
                     MGYPPNNEAYIEEVRQAGLALFSEENLQILNEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ROI21_002658"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ROI21_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDX5111564.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     FAYYVSYRLGGYEGALLGLALIPALVVIPAVIMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ROI21_002659"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ROI21_002659"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDX5111565.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ROI21_002660"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ROI21_002660"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDX5111566.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDTLQQLGFSLVEGEVDLALPVNNVGDSGAEVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGAIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8177..9439)
                     /gene="wecC"
                     /locus_tag="ROI21_002661"
     CDS             complement(8177..9439)
                     /gene="wecC"
                     /locus_tag="ROI21_002661"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDX5111567.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINKHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHIQFKAISGDNVRQQYVVDAKGVWR"
     gene            complement(9436..10566)
                     /gene="wecB"
                     /locus_tag="ROI21_002662"
     CDS             complement(9436..10566)
                     /gene="wecB"
                     /locus_tag="ROI21_002662"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDX5111568.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVMAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(10622..11668)
                     /gene="wzzE"
                     /locus_tag="ROI21_002663"
     CDS             complement(10622..11668)
                     /gene="wzzE"
                     /locus_tag="ROI21_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDX5111569.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMNEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(11680..12783)
                     /gene="wecA"
                     /locus_tag="ROI21_002664"
     CDS             complement(11680..12783)
                     /gene="wecA"
                     /locus_tag="ROI21_002664"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDX5111570.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(13011..14270)
                     /gene="rho"
                     /locus_tag="ROI21_002665"
     CDS             complement(13011..14270)
                     /gene="rho"
                     /locus_tag="ROI21_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDX5111571.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(14355..14456)
                     /gene="rhoL"
                     /locus_tag="ROI21_002666"
     CDS             complement(14355..14456)
                     /gene="rhoL"
                     /locus_tag="ROI21_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDX5111572.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(14597..14926)
                     /gene="trxA"
                     /locus_tag="ROI21_002667"
     CDS             complement(14597..14926)
                     /gene="trxA"
                     /locus_tag="ROI21_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDX5111573.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            15057..16322
                     /gene="rhlB"
                     /locus_tag="ROI21_002668"
     CDS             15057..16322
                     /gene="rhlB"
                     /locus_tag="ROI21_002668"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDX5111574.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLADATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYTKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            16458..17942
                     /gene="gppA"
                     /locus_tag="ROI21_002669"
     CDS             16458..17942
                     /gene="gppA"
                     /locus_tag="ROI21_002669"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDX5111575.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVKNEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(17989..20010)
                     /gene="rep"
                     /locus_tag="ROI21_002670"
     CDS             complement(17989..20010)
                     /gene="rep"
                     /locus_tag="ROI21_002670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDX5111576.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESDEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            20228..20677
                     /locus_tag="ROI21_002671"
     CDS             20228..20677
                     /locus_tag="ROI21_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDX5111577.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDMHFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            20829..21110
                     /gene="ppiC"
                     /locus_tag="ROI21_002672"
     CDS             20829..21110
                     /gene="ppiC"
                     /locus_tag="ROI21_002672"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDX5111578.1"
                     /translation="MAKTAAALHILVKEEKLALELLEQIKNGADLGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(21156..22631)
                     /gene="ilvC"
                     /locus_tag="ROI21_002673"
     CDS             complement(21156..22631)
                     /gene="ilvC"
                     /locus_tag="ROI21_002673"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDX5111579.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            22781..23671
                     /gene="ilvY"
                     /locus_tag="ROI21_002674"
     CDS             22781..23671
                     /gene="ilvY"
                     /locus_tag="ROI21_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDX5111580.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(23668..25212)
                     /gene="ilvA"
                     /locus_tag="ROI21_002675"
     CDS             complement(23668..25212)
                     /gene="ilvA"
                     /locus_tag="ROI21_002675"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDX5111581.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPIDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(25215..27065)
                     /gene="ilvD"
                     /locus_tag="ROI21_002676"
     CDS             complement(25215..27065)
                     /gene="ilvD"
                     /locus_tag="ROI21_002676"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDX5111582.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(27130..28059)
                     /gene="ilvE"
                     /locus_tag="ROI21_002677"
     CDS             complement(27130..28059)
                     /gene="ilvE"
                     /locus_tag="ROI21_002677"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDX5111583.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(28079..28342)
                     /gene="ilvM"
                     /locus_tag="ROI21_002678"
     CDS             complement(28079..28342)
                     /gene="ilvM"
                     /locus_tag="ROI21_002678"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDX5111584.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(28339..29985)
                     /locus_tag="ROI21_002679"
     CDS             complement(28339..29985)
                     /locus_tag="ROI21_002679"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDX5111585.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALLQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(29988..30038)
                     /locus_tag="ROI21_002680"
     CDS             complement(29988..30038)
                     /locus_tag="ROI21_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111586.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(30125..30223)
                     /gene="ilvL"
                     /locus_tag="ROI21_002681"
     CDS             complement(30125..30223)
                     /gene="ilvL"
                     /locus_tag="ROI21_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDX5111587.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            30576..32096
                     /locus_tag="ROI21_002682"
     CDS             30576..32096
                     /locus_tag="ROI21_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDX5111588.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(32121..32459)
                     /gene="maoP"
                     /locus_tag="ROI21_002683"
     CDS             complement(32121..32459)
                     /gene="maoP"
                     /locus_tag="ROI21_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDX5111589.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            32578..33417
                     /gene="hdfR"
                     /locus_tag="ROI21_002684"
     CDS             32578..33417
                     /gene="hdfR"
                     /locus_tag="ROI21_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDX5111590.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRVRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYKNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(33512..33587)
                     /locus_tag="ROI21_002685"
     tRNA            complement(33512..33587)
                     /locus_tag="ROI21_002685"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33552..33554),aa:Trp,seq:cca)
BASE COUNT         8235 a         9116 c         8520 g         7743 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgcg
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaattt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttga cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgcgcacgac gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattaacag gatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa ctttcgccat cgccgtcggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaatg ccgattttgg cgaaagtcag
      901 tacgaacggg ctgccgttgc tgcctatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggattcg ccacagcacc ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccccccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 tgtgagatag ccaaagaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaataaca tttcgcccat tgaacgcatg atgaaaaaga cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 gaaggcgacg aagtgaccac tccgtcgctt tacagagaga ggaaaatcag aggttgccgg
     1921 tgtaatgcca gcgtaaataa cgcagcaaac gaagctgacg cttaaggcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagcccca gcgtttgcca gattttcggt gcgcgtttca
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg gctgccaacg atattcacat tccactggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg tgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatccgccc cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttca gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcgaacag ataatcgagg gcgtgctgca
     2581 tatcacgcca accgatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgttcgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagaccacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccga agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcaggc caaccacaat
     2941 cgccccgagc gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agcccggagt ggttattcag cacttcccag gtaaagtagt tggcggtgtt
     3061 cagtaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaatggga gccagaccct ggaagtcgat gttgtcgtag ttctgcaaca gcaatgccag
     3181 attctcccac ggcgaaaagg tatcgcgggt gagatagaga aacgtataga acgcttcatc
     3241 accgctcaca ttcattccat agcgttttag cgccagccag aacatgccca caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggagaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgacgccc atcaggatca cccacgtgag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcgg caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacgtagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaaacgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagaca acaaacaggc cactgaattg cagcagactc atgcaacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgcaga tagttcgggc taaagaaggc aatggtgttt
     4081 ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcaccggcaa atgctgttcc gtcatatcct gccagaacgg attttcccgg
     4201 ttaagcacac aaggaatgcc cgcctgaatt agtaagcaca gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgttcagaat ttgcagattt tcttcgctga ataacgccag ccccgcctga
     4381 cgaacttcct cgatgtacgc ttcgttatta ggcggatatc ccatcggcgc caccactttt
     4441 accgtatcgc caaattgctg atgaacggcg cgcaaggcag caatatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt ttccctttac gttgccgatc attcgccatc
     4561 atattgagtg aagggtccat ccgcgtcggg aagtacagca gttcgccccg cacctttgga
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcgtac
     4741 aggtctgccc cccagatatg ccagtaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaagaaaa aacgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcgttg aaaaaccgca gaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataggttgc catataggcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgccgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacctcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccagg ccgttatccc
     5701 agctgggttt cagatagctt aacgggattg caccacgctt aattagcatg atgacggcag
     5761 gaatcaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcaaaaacgc caatcaagct gccgacaatc agggataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcaacgccag taacaggttg ccccaggcaa
     5941 tccccatttg caccagcgcc actaaacgca ccagtccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgct gctgccagca caaaaaccag cgccattagc gtagagaagc
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgggga ttatcatggt
     6121 actgagcaac gtatttggtt acgccattaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcactc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg tgctaacgca
     6661 tcgtagtagt tttgccacag cgccagacgt tgctgattga tacgatctgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc gatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggtttgtgcc tttttcacgg
     6841 atgatctcgg ctcgttcgat caacgcttta tcgttaatca gcgtcgcgcc gccttcaccg
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcag catcttccac cacaaacaga
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaaa acgatttttg cgccacgcag cacaaaggca
     7201 ttggcagtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atctgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atagcgccgg acgccgcacg taaaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta atgcgggaat
     7861 atctgtctct tgtgccactt ctgcgccgct atcaccgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggtatcca gttcacccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacgcgt ctggcgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacaat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcattgtg tgactccctc aaaacagtca
     8161 ttcacggtaa tcagtatcag cgccagactc ctttagcatc gacgacatat tgctgacgaa
     8221 cgttgtcgcc gctgatggct ttgaactgga tatgatcgac cagcatcacc agcacgtctg
     8281 ccgttgccag cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca
     8341 actggtggat gttaggctca acaaccagcg tttcgccgct atgccactgg gcgatcagtt
     8401 cagcgatttc catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac
     8461 caaagcaggc gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa
     8521 ccgccgcttt cacctgatcg ataacccaga acggtttgtg atcgttcact tcgcgcgcag
     8581 tacggataag ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcga
     8641 tgcagtgacc gcccacgcca gggccaggct gaagaatatt gacgcgaggg tggcgattcg
     8701 ccaggcgaat cagttcccag acgttaattc cctgatcggc acaaatcagc gacagttcgt
     8761 tagcaaaagc gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg
     8821 tccgcgagtt agtgacgaca cactcacctt cgaggaaaat tttgtacagt tcgctggcgc
     8881 gggccgaaca aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca
     8941 ttacctggcc cggtaacacg cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc
     9001 ccacctgctg cgggaaagtg agatctggac gcatctctgc taaccattct gccatcttct
     9061 cggttgaccc caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg
     9121 gcgcaatgga gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa
     9181 acggcgtggg tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta
     9241 aaaaaccgcc ttctacggca gtttttacta cgctcgccaa atcaggttcg acgatatgga
     9301 tttcgccacg attgatggta tcaaccgcat gtttgttgat atcgacacca atcacctgtt
     9361 tttgccgaga ggcaaacgca gctgctgtag gcagcccgat gtaacccagt ccgataacag
     9421 aaatggtcgc aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga
     9481 gcatgcctga ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc
     9541 gtcttttaaa agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg
     9601 caccgtaccc gccatcaccg cttccggacg ctcagtggta tcgcgcatta ccagcaccgg
     9661 tttccccagc gaaggcgctt cttcctgaat gccgcctgag tcggtcaaaa tcagccaggc
     9721 gtggttcatc agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt
     9781 cacgtgcccc agaatgcgat tgaccggctc tctgacgttc gggttgagat gcactggata
     9841 gacaatctgg atgtcctggt gcgtggtggc gatgtctgcc agcgcatggc agatttcttc
     9901 aaagccacga ccgaagctct cacgcctgtg accggtcacc agaatcattt ttttattggg
     9961 gtcgataaac gggtaatttg ccgccagttc tgaacgcagc gtatcgctgc tcatcacctg
    10021 gtcacgcacc cataacagtg catcaataac tgtattaccg gtaatgaaga ttcggctgtc
    10081 agcaacattt tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat
    10141 cgccagatgc ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagatcgcc
    10201 cgtgcgcaga ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct
    10261 ggttgccagc gttgtagtag tatcgccgtg aaccagcacg acgtctggtt tgaactcggc
    10321 aagaattggt tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg
    10381 ctgcattatg ttgagatcgt agtcaggtac aatggaaaag agtttcagca cctgatcgag
    10441 catctcccga tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa
    10501 cgcatgcacc aacggcgcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac
    10561 tttcacatcg attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt
    10621 gctatttcga gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc
    10681 cccacataat catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg
    10741 tacgcaaata gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt
    10801 ttaacatggc ccgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat
    10861 tttccagtcg agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt
    10921 cctcggcagg cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg
    10981 cctgttcaat actgttcatc cggcggtcgt agatagcttt cgccacctct tcctgacgct
    11041 tcacctgagc tttcatctgg atggtacgtg ccgcccatgc acctttcagc tcatcattca
    11101 gatggctggc tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt
    11161 caggcgcggt ttcggcaatc agcttcacgc tgtcattgac cgcgcgggta aagtctccgg
    11221 ggataaactg gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc
    11281 ccaccatccg ctgcttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg
    11341 aggccagttg cataacaaac tctttatagg cttcgttcat gaccgatggt tggtcggcag
    11401 aagccatgtt tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc
    11461 ccagcatatt caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa
    11521 aaaacgtata cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct
    11581 tcccagccca caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg
    11641 ccggtttccc aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct
    11701 gccaccacga tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca
    11761 ggcacgctta atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac
    11821 ccactccggg acaaaatgag aatattctgc cagcacccca atggaggcga gcagagctgc
    11881 ggcaagggta atcagcacaa acgcctggcg ggaagtaaac ccggcacgca tgatcaaatg
    11941 gtgaatatgc tggcgatcag gagagaacgg actcatgcct ttacgcaggc gacggtacat
    12001 aatcgccacc atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat
    12061 gggatgggtt ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag
    12121 cgtactgccc gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag
    12181 catgatgtat ggcaggatgg cggcgatcat cgcaaaacac cagattgcaa ggctggtttg
    12241 cccgtcgaac cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag
    12301 caagccatca atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag
    12361 cgtcaggaag tagccaaacg gtccgagcac catctcccag gagccaaaga tataacccag
    12421 gctactgaga taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt
    12481 ggcacggatt tttacgctga tatcaaaacg gtcatccagc gcgccaatga aaacaagcac
    12541 accggcacaa gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt
    12601 gaagcaaatc cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg
    12661 tttgcggaag ttaggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa
    12721 cagaaacagt gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt
    12781 cacaatgcat gctctcagag aaaattatta gcagaagtat aaccacgaag gccattattc
    12841 agaagggaaa ctctgctctg aacccaccaa ccttgtgcaa aaaacaacca cgtgattcgt
    12901 gttatgcgca ataaacaaac ataactcgta tttgttataa cgccgttaag attacagata
    12961 taaaaacaaa acgccacgta aacacgtggc gtttttggca taagacaaat ttatgagcgt
    13021 ttcatcattt cgaagaaatc gtcattggtc ttggtcattg ccagtttatt aatgaggaat
    13081 tccattgcat cgatttcgcc catcgggtga atgattttgc gcaggatcca cattttctgc
    13141 agttcttcct gagtagtgag cagctcttct ttacgggtac cggaacggtt gtagtcgatt
    13201 gccgggaaga cgcgtttttc agcgatctta cgagagaggt gcagttccat gttgcctgta
    13261 cctttaaact cttcgtagat aacttcgtcc atcttggaac cggtatcgat aagcgcggta
    13321 gcgatgatgg tcaggctgcc gccctcttcc acgttacgcg ccgcaccgaa gaagcgtttc
    13381 ggacgatgca gggcgttggc atccacacca ccggtcagca ctttacctga cgccggaaca
    13441 acggtgttgt aagcgcgcgc cagacgagtg atggagtcga gcagaatgat aacgtctttc
    13501 ttgtgctcaa ccaggcgttt cgccttctcg atcaccattt ccgcaacctg aacgtggcga
    13561 gatgcgggtt cgtcaaaggt agaagcaaca acttcacctt taaccagacg ctgcatctcg
    13621 gttacttctt ccggacgttc gtcgatcagc agaaccatca gtacgcaatc cgggtggttg
    13681 taagcaatgc tctgagcaat gttctgcagc agcatggttt taccggcttt cggcggtgcc
    13741 acaatcagac cacgctgacc acgaccgata ggtgatgcca gatccagtac gcgagcggtt
    13801 aaatcttccg tagaaccgtt accacgttcc atacgcagac gagagtttgc gtgcagcggg
    13861 gttaagttct caaagaggat tttgttgcgg gcgttttcag gtttgtcgaa gttaacttcg
    13921 ttaactttca gcagcgcaaa atagcgttca ccttctttcg gcgggcgaat cttaccagag
    13981 atggtatcac cagtgcggag gttgaaacgg cggatttggc taggggaaac gtagatgtca
    14041 tcaggaccgg cgaggtagga gctgtctgcg gaacggagga aaccaaatcc atcctgcaat
    14101 atctccagta cgccatcacc aaagatatct tcgccactct ttgcgtgctg cttcaggatg
    14161 gcaaaaataa tgtcctgctt acgcatacga gccaggtttt ccagccccat attttcgccg
    14221 agagtgatca gctcagaaac cggcgtattc tttaattcgg taagattcat agtggtgtga
    14281 gttcttaaac ttggggtaaa tctcgaactt aatgttgtga atggtatggc aggatcatcc
    14341 atgcctgttt acggtcatcg actcatgtct tttcgctgcc tggtcacagg agagtacgca
    14401 gaactgaaac gacaagacgg attgagtgac gagccagaaa tctgttcact tcgcatttaa
    14461 gataaaacgg gaagcgttgg aaattacaag attcaaactt aataaggtat gtttaatacg
    14521 aagtcaacac taagttagca tgactcacgc cgggcgtcca gtttttagcg acggggcacc
    14581 cgaacatgaa attcccttac gccaggttag cgtcgaggaa ctctttcaac tgacctttag
    14641 acagtgcgcc cactttggtt gccgccactt caccgttttt gaacagcagc agagtcggga
    14701 taccacggat gccatatttc ggcgcagtgc cagggttttg atcgatgttc agttttgcaa
    14761 cggtcagttt gccctgatat tcgtcagcga tttcatccag aatcggggcg atcattttgc
    14821 acggaccgca ccactctgcc cagaaatcga cgaggatcgc cccgtccgct ttgagtacat
    14881 ccgtgtcaaa actgtcgtca gtcaggtgaa taattttatc gctcatatat aactccacag
    14941 gaataagcct ggcgtgttgg tttcgttgtt ggtgtaacat taaccaacta aaggttgact
    15001 ttatttcacc ggatacgctt tcgtaaagca atagtaagct gatattctac cacactatga
    15061 gcaaaacaca tttaacagaa cagaagtttt ccgacttcgc cctgcatccg aaggttgtag
    15121 aagcccttga aaaaaaaggg tttcataact gtacgcccat tcaggcactg gcccttccgt
    15181 tgacgctggc gggtcgtgac gtagccgggc aggcgcaaac cggtaccggg aaaacgatgg
    15241 cgtttcttac gtcaacgttt cattatcttc tctctcatcc cgcgattgcc gatcgcaagg
    15301 tgaatcaacc gcgtgcctta attatggcac cgacgcgtga acttgccgtg cagatccacg
    15361 ccgacgcgga accgctggcg gatgcaaccg gcctgaagct gggtctggct tacggtggtg
    15421 atggctacga caaacagctg aaagtgctgg aaagcggcgt tgacattctg attggcacca
    15481 cggggcgttt aattgactac accaagcaga accacattaa cctcggtgcc attcaggtag
    15541 tggtactgga cgaagccgat cgtatgtacg atctgggctt tattaaagat atccgctggc
    15601 tgttccgccg tatgccgcct gcaaaccagc gcctcaacat gctgttctcc gccacgcttt
    15661 cgtaccgggt acgtgaactg gcgttcgagc agatgaacaa tgccgaatat attgaagtgg
    15721 aaccggaaca gaaaacgggc caccgtatta aagaagagct tttctaccct tctaacgaag
    15781 aaaaaatgcg tttgctgcaa acgctgatcg aagaagagtg gccagacaga gcgattattt
    15841 tcgccaacac caaacaccgt tgtgaagaga tctggggcca cctggcggca gatggtcatc
    15901 gtgtcggttt gttgacaggc gatgtcgcgc agaaaaaacg tctgcgtatt cttgatgaat
    15961 ttacccgtgg ggatctggat attctggttg ccaccgacgt tgccgcgcgt ggcctgcata
    16021 tcccggctgt aacgcacgtc tttaactacg atttacccga tgactgtgaa gattacgttc
    16081 accgtattgg tcgtacaggt cgtgcaggcg caagtggtca ttccatcagc ctggcatgtg
    16141 aagagtatgc attgaatttg cctgctattg agacctatat tggtcactca attccggtaa
    16201 gcaaatacaa tccggacgca ttgatgaccg atctgcctaa accgctgcgc ctcacgcgcc
    16261 cgcgcacagg caatggtccg cgtcgtactg gtgctccgcg taatcgtcgt cgttcaggtt
    16321 aaataaaaaa tgtcatactc aaaatcattc aaactgtatc aagatggcca gagaatgaac
    16381 cccgatgagc ttactgtagt aagtgattca ggtgaaagaa tgcagccaac acagagacag
    16441 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    16501 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    16561 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgcc ctctctaatg
    16621 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    16681 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    16741 attttattgc caaagcacag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    16801 aggaagcacg tctgatttat cagggcgttg ctcacacaac tggcggtgcc gatcagcgcc
    16861 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    16921 cctcgttgtt cagcctgtcg atgggctgtg tcacctggct ggaacgctat tttgccgatc
    16981 gtaatctggg gcaggaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    17041 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggcgct tccggcaccg
    17101 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    17161 agttgcagca actgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    17221 acgggctgac gctggaacgt gcgttagtgt tcccgagtgg tctggcaatc ctgatagcca
    17281 tttttaccga actgaatatt cagtgtatga ccctggcggg tggtgcgctg cgtgaaggcc
    17341 tggtctacgg catgttgcat ctgaccgtcg agcaggatat tcgcagccgt acgctgcgta
    17401 atattcagcg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    17461 ctaacttctt cgatcaggtg aaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    17521 tcatcagcgc ctgccagctt catgaaatcg gcctgagcgt tgacttcaaa caagcgccgc
    17581 aacacgctgc ttatctggta cgtaatctgg atcttcccgg ttttaccccc gcacagaaaa
    17641 aactgctggc gacgctactg ctcaaccaga cgaatccggt cgatctctca tcgctgcatc
    17701 agcaaaatgc cgtaccgccg cgcgtcgcag aacaactctg ccgtttacta cgcctggcca
    17761 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    17821 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    17881 agattattgc tcaggaaagc cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    17941 aatgagtaag tgccggatgc gatgctgacg catcttttcc ggccttgatt atttccctcg
    18001 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    18061 ttcggcgctg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    18121 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    18181 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    18241 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    18301 catgtagaca taaggaaact ccagcccttt cgacgcgtgc agagtcatca gttgtacctg
    18361 atctaactct tcatcactct cgccgcgctc catcatgtcg cgcagagtaa agcgcgtcac
    18421 cacctgggtg agcgtcatcg gctcatccag ttcgctacct tccagcatct ccgtcatcca
    18481 gctaaacagt tggttgacgt tcttcatgcg catttcggcg gctttcgggc tgggcgatgt
    18541 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgtaccg cggcaatcgg
    18601 ctcccgctcc gccagacgct ggatttctgc caaccaatga gtgaagcggg tcaatgcttc
    18661 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    18721 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtagccgggc caatctctcg
    18781 cttcggcgtg ttaacaatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    18841 cagataagcc agcaaatcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    18901 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatggttacc
    18961 gcgataaaga atggcgtaat ctttgtactg cgttttattg acgaagtgat gcgcgatcag
    19021 ctcgccggtg actctctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    19081 cgcgccatag cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcaat
    19141 gaggatgttc gccgctttca gaatacgtcc ggaagagcgg tagttctgct caagcttaat
    19201 caccttcagt gccgggaaat cctgactcag cagcaccagg ttttgcggac gtgcaccgcg
    19261 ccaggagtag atcgactggt cgtcatcgcc aactacggta aagcgcgcgc ggctgcccac
    19321 cagcagtttc accagttcat actggctggt gttggtgtcc tgatactcat ccaccagcag
    19381 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gcagcaacgt
    19441 cggcaataaa atcagatcat cgaagtcgag aacgttacag gctttcaggt gtgcatcata
    19501 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    19561 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcagct gttgcaggag
    19621 aactttgtca tcttcaatca gcccctcggt cagctcttta agcaaagcaa gctgatcggt
    19681 atcgtcaaac aacgagaagt ttgctttcat cccaagcgcc gcatactcgc gtttgatgat
    19741 atccagcccc agtgtgtgga aggtggagat catcagccca cgcgcctctt tgcgacccaa
    19801 cgtctgcccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    19861 gatatgtcgc gcctgataac cgcaaccgcg gatcagatgg gcgattttat tggtgatgac
    19921 acgagtttta ccggaacccg cgcctgccag caccaggcag gggccggtaa cgaattcgac
    19981 agcttgttgt tggccggggt ttagacgcat aggtgtattg ctcaatcttc gaacgggggg
    20041 gaggattgta gcagaatcgc atttgaatgt gaccgagtga aaaccgctaa ccggatggct
    20101 gcggatgttt aaatcagaga gggaaggccg aaaccggagt tcatcatgaa tccattagac
    20161 ttagaaaata tcgggtcgta acgttgtgtt attacttttt cagagtttac taaggagagg
    20221 tttactcgtg gcgattaatt tcagccccaa ggttggtgaa atactggaat gcaactttgg
    20281 taactatcct gttagccaga atggacagtt cagcacgacg tattacgatg gccgcattcc
    20341 acctgagatg ataaaaaata ggttggttgt cgtgctcaac ggaaaaatta atggcaacgc
    20401 atgtatcgtc gttccattat caaccactcg cgaccatgac aagttaaatc gtggaatgca
    20461 tgttgaaata gccagtaatg ttatcaatga catgcatttc tttgatcagc agatacgttg
    20521 ggctaaagcc gacctggtac aacaagtcag tcgaaaccga ttaaacagag caaggactta
    20581 tcgggggtat cttaatcaat gtttgccaca tgagttggtt gcagacatac agagggcagt
    20641 catcaagtca atcaatgcca tatcgctcat aaattgacaa taaaatcacc atccccgata
    20701 atgactctgt agcccataga ggccctaagt tttctcccct tgagggacct gcgaagcccg
    20761 gattttccgg gcttttgcca tatatgtccc ccctccctga ctgacaatgt ttaattcaag
    20821 gcacgatcat ggcaaaaaca gcagctgcac tgcatatcct tgtaaaagaa gagaaactgg
    20881 ctctggaact tctcgagcag attaagaacg gtgccgattt aggcaagctg gcgaagaaac
    20941 actccatttg cccgtcaggt aaacgcggtg gtgatttagg tgaattccgc cagggccaga
    21001 tggttccggc gttcgataaa gtggttttct cttgtccggt actggagcca accggtccgc
    21061 tgcataccca gttcgggtat cacatcatta aggtgcttta ccgtaactaa cagcaaggcc
    21121 ttcttcagga gaaggccttg aactctgaat cgcacttaac ccgcaacagc aatgcgtttc
    21181 atatctgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gctgcgaatc
    21241 gcttcgttca catcacgcaa ctgcgcgtta tctaccgcac cttccggaat agctttaccc
    21301 aggtcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
    21361 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
    21421 ttacgagcga tagtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagac
    21481 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca actcaacgcc tgcttttacc
    21541 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
    21601 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
    21661 tcgtcgttgg cccagtcagc catcatgccg gaggagaatt cgccagagat gatgtcgtcc
    21721 atatgtttct ggaacagcgg tgccatgatc tctttcagct gttcagacag cgcgtaagca
    21781 cgcagtttcg ccgggttaga aagacggtcc atcatcaggg tgatgccgcc ctgtttcagc
    21841 gcttcggtga tggtttccca accgaactga atcagttttt ctgcgtatgc cggatcggtg
    21901 ccttcttcca ccagtttgtc gaagcacagc agagaaccag cctgcaacat accgcacagg
    21961 atggtttgct cgcccatcag gtcagatttc acttccgcaa caaaagagga ttccagcacg
    22021 cccgcacggt gaccgccggt tgcagccgcc catgctttag cgatcgccat gccttcgcct
    22081 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtat
    22141 tcttcacgca cttcggtgcc agggcatttc ggcgcaacca ttactacggt gatgtcttta
    22201 cggatctgct cgcctacttc gacgatgtta aaaccgtgcg agtaacccag cgccgcgccg
    22261 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc cggcgtcagg
    22321 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccattt
    22381 tcggtcgctt tacgccagga tgcgcgcttc tcggcaatcg cttctttacg cagagcgtaa
    22441 gaaatatcca gaccagaatc acgcatgttc aggccctggt tcagaccctg tgcaccacaa
    22501 ccgacgatga ctactttttt accctgaagg tagctcgcgc catcggcgaa ttcatcgcgc
    22561 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcagattcag tgtattgaag
    22621 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
    22681 agaatgaatt cactatatga caggaaattc attgcggaaa ttgatatatt cacaacatca
    22741 cattgcaatt tctgcaacgc caacattgag ggctgcgcca ttggatttac gcgatctgaa
    22801 aaccttcctg catctggcgg aaagccgcca ttttggccgt agcgcgcgag caatgcacgt
    22861 tagcccgtct acgctttcgc gacaaataca gcgcctggaa gaagatctcg gtcagccgct
    22921 gtttgtgcgt gataaccgca cggtaacgct gacggaagcg ggcgaagaac tgcgcgtttt
    22981 cgcccagcaa acgctgttgc aatatcagca attgcgtcac accatcgatc agcaagggcc
    23041 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
    23101 gccgattctg gatcgcttcc gcgcggaaca cccgtcagtg gagatcaaac tcactaccgg
    23161 tgatgcggcg gatgcaatgg aaaaggtggt cactggcgaa gcagatttgg cgattgccgg
    23221 aaaaccggaa accttgcccg gcgcggtggc attttcgatg ctggagaatc tggcggtggt
    23281 gctgattgct cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
    23341 ctggtctaca gtgccgttta tcatggccga tcagggaccg gtacgccgcc gcattgaact
    23401 gtggtttcgg cgtaacaaaa tcagcaaccc aatgatttac gccacggttg gcgggcatga
    23461 agcgatggta tcgatggtgg cgctcggctg tggcgtggcg ctgttgccgg aagtggtgct
    23521 ggataacagc ccggaaccgg tgcgtaaccg cgtgatgatt ttagagcgca gtgatgaaaa
    23581 aacgccgttt gagctgggcg tgtgtgcgca aaaaaagcgg ctgcatgagc cgctgattga
    23641 ggcattctgg aagattttgc cgaaccacta acccgccaaa aagaacctga acgccgggtt
    23701 attggtttcg tcgtggcaat cgtagcccag ttcattcagc cgggtttcaa aatccggctc
    23761 atgatcgcca agctcgaacg ccgccagtac gcgcccgtag tcggtgccat ggctgcgata
    23821 atggaacaaa gaaatgttcc agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc
    23881 cggtgattcc gggaattcga agctgtagag gcgttcctgc aacggatgcg atggacgccc
    23941 gccaaccata tagcgcacat gcagcttcgc catttcgtcg tcggagagat caaccacgct
    24001 gtagccaccg tcgttgagca tctgcaaaat ttctttgcgc tcttcgagac cgcggctcag
    24061 gcgcacgccg acaaaaatgc aggcgttttt ggcatcagca aagcggtagt tgaactcggt
    24121 gaccgagcgc ccaccaagta actggcagaa tttgaggaag ctgccctttt cttccggaat
    24181 ggtcaccgcc agtaacgctt cacgctgttc gcccagttcg cagcgttccg agacgtagcg
    24241 cagaccgtga aagttcacgt tggcaccgga aagaatatgc gccagccgtt caccgcgaat
    24301 gttgtgctgg gcgatgtatt ttttcatccc cgccagcgcc agcgcgccgg agggttccgc
    24361 cactgcgcgc acatcttcga acaggtcttt catcgccgca cagatggcat cgctatcgac
    24421 ggtgatgatg tcgtcaagat actcctggca caaacggaag gtttcatcgc cgatgcgttt
    24481 taccgcgacg ccttcagcaa acagccccac gcggggcaga tcaatgggat gacctgcatc
    24541 aagtgccgct ttcaggcagg cggaatcttc cgcttccacg gcgatcactt tgatttgtgg
    24601 catcagttgt ttgataagta ccgcgacgcc cgccgccaga ccgccgccgc cgaccggtac
    24661 aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc agcgccagcg tgccttgccc
    24721 ggcgatcacc atcggatggt cgaacggcgg cacccaggtg aagccctgct gctgtgacag
    24781 ttcgatcgct ttcgctttcg cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc
    24841 aaagccgcgc accgcatcaa ctttgatatc ggcggtggcg gttggcatga cgatcagcgc
    24901 cttcacgcct aaccgtgcgg aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc
    24961 ggtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc
    25021 gcgcagctta aagctgtgta ctggctggcg atcttcgcgc ttcaccagaa tcacgttatc
    25081 aagacgcgac gacagttttt ccattttctg tagcggcgtg acctgcgccg cttcgtagac
    25141 cggcgcgcgc agcaccgctc ttaaatattc ggcaccttca ggggcaccgg acaggggttg
    25201 tgagtctgcc atcattaacc ccccagtttc gatttatcgc gcaccgcgcc tttgtcggcg
    25261 ctggttgcca ggctggcgta ggcacgcagc gcaaaggaaa cctggcgttc acggtttttc
    25321 ggcgtccagg ctttgtcgcc acgagcttcc tgcgcttcac gacgcgccgc cagttcggca
    25381 tcgcttacct gtaactgaat gccacggttc ggaatgtcga tagcgatcag gtcgccgtct
    25441 tcaatcaggc caatgctacc gccgcttgcc gcttccggcg agacgtggcc gatggaaaga
    25501 ccagaggtgc cgccggagaa gcgtccgtcg gtgatcagcg cgcaggcttt accgagcccc
    25561 attgatttca ggaagctggt tgggtagagc atttcctgca tgccggggcc gcctttcggg
    25621 ccttcgtaac ggatcaccac cacatcgcca gcgacaactt tgccgccgag aatcgcttct
    25681 accgcgtcgt cctggctttc gtacactttc gccggaccgg tgaatttgag gatgctgtca
    25741 tcgacgcccg cggttttaac gatgcagccg ttttctgcga aattaccgta cagcaccgca
    25801 aggccgccgt ctttgctata ggcgtgttcc agcgagcgga tacagccatt ggcgcgatcg
    25861 tcgtccagcg tatcccaacg gcaatcttgc gagaatgcct gggtggtacg gatacccgca
    25921 gggccggcgc ggaacatatt ttttaccgcg tcatcctgag tcagcataac gtcgtattgt
    25981 tccagcgttt gcggcaacgt caggccaagt acgtttttca catcacggtt cagtaacccc
    26041 gcgcgatcca gctcgccgag aataccgata acaccaccag cccggtgaac atcttccata
    26101 tggtattttt gggtgctcgg cgcaacttta cacagctgcg gaaccttgcg ggaaagctta
    26161 tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt
    26221 acggtgttgg tagagccgcc catcgcgata tcgagcgtca tggcgttttc aaacgccgcc
    26281 ttactggcga tattacgcgg cagtgcactt tcgtcatttt gctcgtagta acgtttggtc
    26341 aattcaacaa tgcgtttacc ggcattgagg aacagctgct tacggtcggc gtgagttgcc
    26401 agcagcgagc cgttgcccgg cagcgacagg cccagcgctt cggtcaggca gttcattgag
    26461 ttagcggtaa acatcccgga gcaggaaccg caggtcggac acgcggaacg ttcaacctga
    26521 tcgctctggg agtcagatac tttcgggtct gcgccctgga tcatcgcatc aaccagatcg
    26581 agcttgatga tctgatcgga cagtttggtt ttcccggcct ccatcgggcc gccggaaaca
    26641 aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt gattttgtcg
    26701 cagttagaga tgcagaccat ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa
    26761 tcagcgatca gttcgcgaga tggcagtgaa taaagcatcc ccccgtggcc catggcaatc
    26821 ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc ttcaatttgt
    26881 tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac
    26941 gagttcacaa ccgcgataat cggcttaccg aaatcggcgt cggtcattcc ggtggcgcgc
    27001 cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga acggtactta
    27061 ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtgcgt gccgtcccat
    27121 tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg tttcgccagt
    27181 gaagaggccg aagaaggctt gctgaatgcg tttggtaacc gggccgcaac ggccttcacc
    27241 aacctgaata ccgtctacac tgcgcactgg cgtgatttct gccgcagtac cggacataaa
    27301 cacttcatcc gccaggtaca gtgattcgcg cgacagcacc tgctcacgca cttcaattcc
    27361 cagctctttc gccagtttga tgatggcgtc acgggtaata cccggcagcg cggaggaggt
    27421 gaacggtggg gtgaacagca caccatcttt cacttcaaac aggttttcgc ctgcgccttc
    27481 agagatataa ccgttcacat ccagcgcgat accttcctga taaccgtggc ggcgcgcttc
    27541 gctacccacc agcagggaag agaggtagtt accaccggct tttgccgccg tcgggatggt
    27601 gtttggtgct gcgcggttcc aggaggaaac catcgcatcg atcccctgct ccagcgcttc
    27661 tgcacccaga taggcccccc acgggaaagc ggcaataatc acatcggttg agtatcccgc
    27721 tggcgggttt acgcccatgc caacatcacc gacgaagatc agcggacgga tataggcgct
    27781 ggtgagattg tttttgcgga tcacgtcacg acaagcttcc atcagctcat caatgctctg
    27841 cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat gctcacgatg
    27901 gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa aaaccgaggt
    27961 gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc gaaccatctc
    28021 cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc ttttgcgctc
    28081 aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca
    28141 ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca acggtcaatt
    28201 cgatatttat attttgtgca tcgctggcgg cggccatatt cattgaacag acgtggaaac
    28261 cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc cggattgaag cgagccgata
    28321 cattgacctg atgttgcatc atgataattt ctccaacatt tctgaattac tggcacctgg
    28381 cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca ggtatggccc
    28441 atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg
    28501 gccagggatg ccgaaggcgc tggctaacat gaggaaatcg gggttatcag taagggttgt
    28561 ttcgctgtat cgttcctgga aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg
    28621 gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg
    28681 cacattcatc atgaaagagc cgtcaccgga gatacagacg acggtatcgt tcggtcgcgc
    28741 gacttgtgcg ccaaccgccg ccggtaaacc aaaacccatg gtgcctaatc cgctggaggt
    28801 gatgaagttt tccgggcgag tgtggacgat gtgctgggca gcccacatct ggtgctgccc
    28861 cacatctgtg gtcacgatgc aatccgcagg tttacgatcc gacagttgtt ttaacaacaa
    28921 cggcgcgtag atagcgtcac caggatggtc gtaacgccag gcatgttcat cacgcagttg
    28981 cgcgcagtgt tgctgccagt catcgatatt taacggctgc agtaatgctg gtaacagagc
    29041 atttaaatca ccctgtaatg ccacatgtgc ctgacgcagc ttgttcattt ctgccggatc
    29101 gatatccata tggataacgc tggcgtgtgg tgcgaaggtg ttcagtttgc cggtcacccg
    29161 gtcatcaaaa cgtgcgccca cggcgatcag tagatcgcac tcctgcactg cgaagttcgc
    29221 cgctttggtg ccgtgcatcc ccagcatgcc cagatagtac ggataatctg cttctactgc
    29281 gcccagccct ttcaacgtac aggtggcagg catttttgtg gcagcgagaa attcacgcaa
    29341 agccgaaact gcctgcgcca tgcccacgcc accgccaacg tacagcatcg gtttttgcgc
    29401 ttttgccagc atctgacgcg cttgctcgac ttcggcatgt gggaaagtca cttcgttttc
    29461 aacagtggtg aaccacggtt ccagatcgcc gctggctaac tggatatctt ttggaatatc
    29521 gaccaaaacc ggaccaggac gacctgagct ggcaacgtcg aatgcttcag ccatgatgcg
    29581 cggcaactct tccagcgact gcaccagaaa gctgtgcttg gtacaggcta atgacaatcc
    29641 cagtacatcc acttcctgaa atgcgtcagt gccgataaac ggtgcggata cctgaccggt
    29701 gatagcaaca acggggatgg aatctaacag cgcgtccgca agcccggtta tcaggttggt
    29761 tgcacctgga ccagacgtgg cgatacatac gccagttttg ccggtagcac gggcataacc
    29821 gatagccgcc attgctgcac cttgctcgtg tcggcacagc aagtgttcca cgccaccgtc
    29881 atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa cggtgttcac
    29941 accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta gttccccgtc
    30001 ctgaatcttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc agttatgttt
    30061 ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg ttaaggcttg
    30121 atctctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata atcaccacca
    30181 cgctaatcac gaccaggcta atcactcgta gaagggctgt cattttgtct tttcttgcat
    30241 cttgttcgaa ggaatgccta aagagttacc atagattttg cgaatagcac aagatatttt
    30301 ttggcctatt tttatgatgt ctttaataat ttcaagagaa attgttgttt tttaaaaaga
    30361 aaatagtgag tgaaaaaaat tcaatttcag tatgaatgtt gttcccttcc tcgtagttca
    30421 tcctttgccc gctgcatttt tgcgagcatc attccaccgc cagaacaact caacttcact
    30481 tacacaaata aaccggaatc agccactaaa attcacgaat tagcgggcta cagcagcgat
    30541 atcttatgca cataatggtg actcaaggag ggcttatgtc actgtcaatt gttcataccc
    30601 gcgcagccct gggagtaaat gcgcccccaa tcactgttga ggtacatatc agtaaaggtc
    30661 tacccggctt aacgatggtg ggcttaccag aaacaacggt aaaagaagcc cgcgatcgcg
    30721 tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc accatcaacc
    30781 ttgctccagc cgatctgcca aaagaagggg gacgatatga tttacctatc gccattgcgt
    30841 tgctggcggc ctcagaacag cttacagcca ataaattaga tgaatatgaa ttagtcggag
    30901 aactagcgct tacaggcgct ctgcgtggcg ttcccggcgc aatctccagt gcaactgaag
    30961 ctattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaagatgaa gtggggctaa
    31021 ttaacggtga aggatgcctg atagccgatc atctgcaggc tgtctgtgcg tttctggagg
    31081 gtaagcacgc tctcgaacgc ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg
    31141 atctcagtga tgttgtcggt caggagcaag gaaagcgagg actggaaatt accgccgctg
    31201 gcgggcacaa ccttttactg attgggccgc cgggaacagg taaaacaatg ctcgccagcc
    31261 gtattaatgg ccttttgcca gatttaagca atgaagaggc actggagagc gctgcgatat
    31321 taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgccca ttccgctcac
    31381 cgcatcacag tgcgtcgtta actgcgatgg tgggcggtgg tgcaattcca gggccaggtg
    31441 aaatttcgct ggcgcataac ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc
    31501 gtacactgga tgccttgcga gagccgattg aatccgggca gatccatctt tcacgcacac
    31561 gagcaaaaat aacctatcca gcccgtttcc agcttgttgc ggcgatgaat cccagcccta
    31621 ccggacatta tcagggaaac cataaccgct gcacgccaga acagacatta cgttatctca
    31681 accggctctc ggggcccttt ctcgaccgct tcgatctctc actggagatc ccattaccgc
    31741 cccccggcat tttgagtaaa acggtagtgc cgggagaaaa cagcaccacc gttaaacaac
    31801 gtgtaatggc cgccagagag cgccaattta agcggcagaa taaactgaac gcctggctgg
    31861 ataatccgga aatacgccaa ttctgcaagc ttgagagcga agatgcgcag tggctggaag
    31921 aaacgctgat ccatctgggg ttatcgattc gtgcctggca gcggttattg aaagttgcac
    31981 gaacaattgc tgatattgat cagtctgaca ttatcacacg tcagcatttg caggaggcag
    32041 ttagctatcg tgcgattgac cgtttgctca tccatctgca aaaactactg acataaaaaa
    32101 agggcatttc gcccttttta ttaatcgtca gaatcggtgt agtcttcagc accttcaacc
    32161 tgcggtttac cgccggaaag ggtgtgaaaa cgttttggac gcttgatacg cgtcatatac
    32221 ttggaccaca cgcgttctgc ttctgtcact ggctcacgtt cgccacggca tactgctacg
    32281 aagagtttct cttcctcggt aaccggctcg cgtttgccaa gatccaactc attgaaggca
    32341 taaccatgac gctcaagcag ttgtgcctct ttgatggtga agtcaccatg acgagagaat
    32401 ccacgtggat aatgtttatt gtcgaaatat cgattagtcg tcgtaaagct ttccgccatc
    32461 ctgcacgctc ctaattcttt gaccgagcta gttatggcgc ggagtattag ttacgcttga
    32521 cagagtgtaa aacaaaacat ttaaatcata acgacaaata attttgcgga gagcactgtg
    32581 gatacggaat tgttaaaaac tttcctggaa gttagccgaa cgcgtcactt tggtcgagcg
    32641 gctgaatcgc tctatctgac ccaatcagcc gtgagctttc gagtcagaca actggaaaat
    32701 caactgggtg tgaacctttt cacccgccac agaaacaata tccgtttaac cgctgccggt
    32761 gaaaaactac tgccttatgc agaaacgctc atgagcacct ggcaggccgc ccgtaaggag
    32821 gtggcgcata cctcacgaca taacgagttt tctatcggtg ccagcgcctc attgtgggaa
    32881 tgtatgctta atcagtggct gggacgtttg tataaaaatc aggatgccca tacaggctta
    32941 cagttcgaag cgcgaattgc ccaacggcag tctctggtaa aacagctgca tgaacgccag
    33001 cttgatcttc ttattactac tgaagcgccc aaaatggacg aatttagcag tcagttgctc
    33061 gggtatttca ctttagcgct ttataccagt gccccttcca aactaaaggg agatcttaac
    33121 tatctgcgac tggaatgggg gccagatttt caacagcatg aggcaggttt gatcggtgct
    33181 gacgaagttc ccattctgac aaccagttct gctgaactgg cacagcaaca gattgcgacg
    33241 cttaatggtt gcacctggct acccgtcagt tgggcgcgta aaaaaggcgg cctgcatacc
    33301 gttgtcgata gcacaacact ttcacggccg ctttatgcca tatggctgca aaatagcgat
    33361 aaaaatgcgt tgattcgcga tctattgaaa attaacgtgc tggatgaagt gtattaatct
    33421 gaatggctgg caaggatgcc ggtagaagga tttacttcgg agaggttatt tcagataaaa
    33481 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
    33541 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tagggtggcg
    33601 gaacggacgg gact
//
LOCUS       DAPOZO010000053        32920 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPOZO010000053 DAPOZO010000000
VERSION     DAPOZO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.053"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(254..1273)
                     /locus_tag="ROI21_002686"
     CDS             complement(254..1273)
                     /locus_tag="ROI21_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDX5111591.1"
                     /translation="MKYIPVGVDIAKHLMQVHFVDEHTGEIIDKQVKREKFLEYFSNR
                     EPCLIGMEACGGSHHWARELQKLGHKVRLLKGRFVKAFVMGNKNDVMDARAIWLAVQQ
                     PVKSVAVKSEEQQATLGLHRMRQQLVKFRTAQINALHSLLLEFGETTHKGRAALAKEV
                     PEALERLKERLPLYLINMLDEQYQRITELDEHLDHIEKQLQAIARQNETCQRLLNIPG
                     VGPLIATAAVATMGDASAFKSGREFAAFIGLVPRQTGSGGKIRLLGISKRGDKYLRTL
                     FIHGARAAALLTKEPGPWITELKKRRPASVAIVAMANKLARTVWAIAAHGRKYDKNHV
                     SIRPD"
     gene            complement(1453..1692)
                     /locus_tag="ROI21_002687"
     CDS             complement(1453..1692)
                     /locus_tag="ROI21_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDX5111592.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            1795..2394
                     /gene="nemR"
                     /locus_tag="ROI21_002688"
     CDS             1795..2394
                     /gene="nemR"
                     /locus_tag="ROI21_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDX5111593.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIVLLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            2431..3529
                     /gene="nemA"
                     /locus_tag="ROI21_002689"
                     /pseudo
     CDS             2431..3529
                     /gene="nemA"
                     /locus_tag="ROI21_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            3610..4017
                     /gene="gloA"
                     /locus_tag="ROI21_002690"
     CDS             3610..4017
                     /gene="gloA"
                     /locus_tag="ROI21_002690"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDX5111594.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            4120..4767
                     /gene="rnt"
                     /locus_tag="ROI21_002691"
     CDS             4120..4767
                     /gene="rnt"
                     /locus_tag="ROI21_002691"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDX5111595.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            4860..9476
                     /locus_tag="ROI21_002692"
     CDS             4860..9476
                     /locus_tag="ROI21_002692"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDX5111596.1"
                     /translation="MADNLNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGSDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAVHFE
                     STSGATSNRVQNSDIFISRSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHEVGGISKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPVELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPETLLQIVRDAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVEPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLARRHTGSSEEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTSAEANQANRSALQQAIVTVLADGGAWFAQQISQRIRDKIGESVDPSAMQEAL
                     WALVWQGVITSDIWAPLRALTHSSTNTRTSTRRSHRARRGRPVYAQPVSPRVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQTAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLIPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEIFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(9527..9874)
                     /gene="grxD"
                     /locus_tag="ROI21_002693"
     CDS             complement(9527..9874)
                     /gene="grxD"
                     /locus_tag="ROI21_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDX5111597.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            10209..11036
                     /gene="mepH"
                     /locus_tag="ROI21_002694"
     CDS             10209..11036
                     /gene="mepH"
                     /locus_tag="ROI21_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDX5111598.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTIKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            11164..11745
                     /gene="sodB"
                     /locus_tag="ROI21_002695"
     CDS             11164..11745
                     /gene="sodB"
                     /locus_tag="ROI21_002695"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDX5111599.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(11891..13060)
                     /locus_tag="ROI21_002696"
     CDS             complement(11891..13060)
                     /locus_tag="ROI21_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5111600.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAVLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAKLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(13226..13315)
                     /gene="ynhF"
                     /locus_tag="ROI21_002697"
     CDS             complement(13226..13315)
                     /gene="ynhF"
                     /locus_tag="ROI21_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDX5111601.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            13614..14639
                     /gene="purR"
                     /locus_tag="ROI21_002698"
     CDS             13614..14639
                     /gene="purR"
                     /locus_tag="ROI21_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDX5111602.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(14636..15568)
                     /locus_tag="ROI21_002699"
     CDS             complement(14636..15568)
                     /locus_tag="ROI21_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5111603.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            15681..16892
                     /locus_tag="ROI21_002700"
     CDS             15681..16892
                     /locus_tag="ROI21_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDX5111604.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            17183..18331
                     /gene="cfa"
                     /locus_tag="ROI21_002701"
     CDS             17183..18331
                     /gene="cfa"
                     /locus_tag="ROI21_002701"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDX5111605.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIHVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(18371..19012)
                     /gene="ribE"
                     /locus_tag="ROI21_002702"
     CDS             complement(18371..19012)
                     /gene="ribE"
                     /locus_tag="ROI21_002702"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDX5111606.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            19227..20600
                     /gene="mdtK"
                     /locus_tag="ROI21_002703"
     CDS             19227..20600
                     /gene="mdtK"
                     /locus_tag="ROI21_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDX5111607.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMQNIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            complement(20641..21897)
                     /locus_tag="ROI21_002704"
     CDS             complement(20641..21897)
                     /locus_tag="ROI21_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDX5111608.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGGIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            22206..22282
                     /locus_tag="ROI21_002705"
     tRNA            22206..22282
                     /locus_tag="ROI21_002705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22240..22242,aa:Val,seq:gac)
     gene            22287..22363
                     /locus_tag="ROI21_002706"
     tRNA            22287..22363
                     /locus_tag="ROI21_002706"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22321..22323,aa:Val,seq:gac)
     gene            22472..22777
                     /locus_tag="ROI21_002707"
     CDS             22472..22777
                     /locus_tag="ROI21_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDX5111609.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            22903..24507
                     /locus_tag="ROI21_002708"
     CDS             22903..24507
                     /locus_tag="ROI21_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDX5111610.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCLVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEKVTRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVIFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPGWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDKNPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(24519..25331)
                     /locus_tag="ROI21_002709"
     CDS             complement(24519..25331)
                     /locus_tag="ROI21_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111611.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(25335..26120)
                     /locus_tag="ROI21_002710"
     CDS             complement(25335..26120)
                     /locus_tag="ROI21_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDX5111612.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFGPVWLNIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYIGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(26117..26785)
                     /locus_tag="ROI21_002711"
     CDS             complement(26117..26785)
                     /locus_tag="ROI21_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDX5111613.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHYFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(26849..27487)
                     /locus_tag="ROI21_002712"
     CDS             complement(26849..27487)
                     /locus_tag="ROI21_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDX5111614.1"
                     /translation="MNHQDELPLAEVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGRLVARAVFLSPPYSVAEEELSVLLESIKQNGDHADIACLTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIES
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(27500..29602)
                     /locus_tag="ROI21_002713"
     CDS             complement(27500..29602)
                     /locus_tag="ROI21_002713"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDX5111615.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLNIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPGTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(29623..30249)
                     /locus_tag="ROI21_002714"
     CDS             complement(29623..30249)
                     /locus_tag="ROI21_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDX5111616.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQEEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(30705..30914)
                     /gene="fumD"
                     /locus_tag="ROI21_002715"
     CDS             complement(30705..30914)
                     /gene="fumD"
                     /locus_tag="ROI21_002715"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HDX5111617.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            31471..32883
                     /gene="pykF"
                     /locus_tag="ROI21_002716"
     CDS             31471..32883
                     /gene="pykF"
                     /locus_tag="ROI21_002716"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDX5111618.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         8054 a         8640 c         7970 g         8256 t
ORIGIN      
        1 gcaaaggcat ccattgttct gacatcgacg gtaggttgca gctttatatc cggctttatt
       61 gcagcccgga agctgcgagc ccctatgtaa ttcgccgact cacgcctcat ttcctctgcg
      121 ggctttaggc ccaatcgatt atccaggttt agtgagtcac ggtcttacct gtctttgtca
      181 tcacgctggc tgccagagca acctttctgc attcaccctt ttgtaaagag tttatgttta
      241 tcggtatcag cgattaatca ggcctgatac tgacatgatt tttgtcgtat ttacgtccgt
      301 gagcggctat tgcccacacc gttcgcgcca gcttgtttgc cattgcgacg attgccacgc
      361 tggccggacg acgctttttc agttcggtta tccatggacc aggttctttg gtcagtaatg
      421 ctgccgccct tgcaccatga ataaacaggg ttcgcagata tttatcacca cgtttgctta
      481 ttcccagcag gcggattttt cctcctgagc ctgtctgtct gggcaccaga ccgataaacg
      541 cggcgaattc ccgtcccgac ttaaaggcgg aggcgtctcc catcgtggca acggcagcgg
      601 tggcgattaa cggccccacg cctgggatgt tcagtaaccg ctgacaggtt tcattctgtc
      661 tggctatcgc ctgcaactgc ttctcaatgt ggtcaagatg ttcatcaagt tcagtaattc
      721 gctgatattg ttcatccagc atgtttatca ggtagagcgg tagccgctcc ttcagacgct
      781 ccagggcttc cggcacctcc ttcgccaaag cagcacgccc cttgtgcgtc gtttcaccaa
      841 attccagcaa gagactgtga agcgcgttta tctgtgctgt cctgaacttc accagttgct
      901 ggcgcattcg gtgtaacccc agcgtggcct gttgctcctc gcttttaacg gcgacggatt
      961 ttaccggctg ctgaaccgcc agccagatgg ctcgggcgtc catcacatcg tttttatttc
     1021 ccatcacgaa tgctttgacg aaccggcctt tgagtaatct gaccttatgt cccagtttct
     1081 gaagctcacg cgcccagtga tgagaacccc cacaggcctc catgccgata aggcagggtt
     1141 cacggttgct gaaatattca aggaatttct cacgcttaac ctgcttatcg ataatctctc
     1201 cggtatgttc atcaacgaaa tgcacctgca taagatgttt tgcaatatca acgccgacag
     1261 ggatatattt catggtgtgg atcctccagt ctgttgggag cgaaacgcat cccgttgggc
     1321 acgatgatgc cggaaatctg cggggatcca cactcatcta cttaatcccc gagcggctca
     1381 tttcataatg ttgggatgcg ttcattacat ttcctcttat cagatatgag aggagtatac
     1441 gcaagattag gctcaaaaga gtgatggttg ctccggttcg tctgatgacg ctggcttatt
     1501 tgcacgtaat ttgcgcatta atcgctgccg acaaaggcgc agtacctctt gtttttcgcc
     1561 atcgctcatt ttattccagt taaaacgctc atcccgactg cgaaagcagc cgcgacaaaa
     1621 tccgcgttcg tcagactggc aaattccccg gcacgggctc tggacgggaa agaactctaa
     1681 ttgctccgcc acctcgccct cctcagataa gattattacc actattgaag ctcttaatac
     1741 ccaaagtagc aactttgctt gcactagacc gactggtcta ctacactcca gcgcatgaac
     1801 aaacacaccg aacatgatac tcgcgaacat ctcctggcga cgggcgagca actttgcctg
     1861 caacgtggat tcaccgggat ggggctaagc gaattactaa aaactgctga agtgccgaaa
     1921 gggtccttct atcactactt tcgctctaaa gaagcgtttg gcgttgccat gcttgagcgc
     1981 cattacgccg catatcacca gcgactgact gagttgctgc aatccggcga aggtaactac
     2041 cgcgaccgca tactggctta ttaccagcaa acactgaacc agttttgcca acatggaacc
     2101 atcagtggtt gcctgacagt aaaactctct gccgaagtgt gcgatctgtc agaagatatg
     2161 cgtagcgcga tggataaagg cgctcgcggc gtgatcgttc tgctctcgca ggcgctggaa
     2221 aatggccgtg atagccattg tttaaccttt tgtggcgaac cgctgcaaca ggcacaagtg
     2281 ctttacgcac tatggctggg tgcgaatctg caggccaaaa tttcgcgcaa ttccgagcca
     2341 ctggaaaacg cgctggcaca tgtaaaaacc attattgcga cgcctgccgt ttagcaggca
     2401 ttttttatca ccagacgacc gggagccttt atgtcatctg aaaaactgta ttccccactg
     2461 aaagtgggcg cgatcacggc ggcaaaccgt atttttatgg caccgctgac gcgtctgcgc
     2521 agtattgaac cgggtgacat ccctaccccg ttgatggcgg aatactatcg ccaacgtgcc
     2581 agtgccggtt tgattattag cgaagccacg caaatttctg cccaggcaaa agggtatgct
     2641 ggtgcacctg gcatccatag tccggaacaa attgccgcat ggaaaaaaat taccgctggc
     2701 gttcatgctg aaaatggtca tatggccgta cagctgtggc acaccggacg catttctcac
     2761 gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt ccgcacttag cgcgggaaca
     2821 cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg ttgaaacatc catgccgcgt
     2881 gcgcttgaac tggaagagat tccgggtatc gtcaatgatt tccgtcaggc cattgctaac
     2941 gcgcgtgaag ccggttttga tctggtagag ctccactctg ctcacggtta tttgctgcat
     3001 cagttccttt ctccttcttc aaaccatcgt accgatcagt acggcggcag tgtggaaaat
     3061 cgcacgcgac tggtgctgga agtggtcgat gccgggattg aagaatgggg tgccgaacgc
     3121 attggcattc gcgtttcgcc aatcggtact ttccagaaca cagataacgg cccgaatgaa
     3181 gaagccgatg cactgtatct gattgaacaa ctgggtaaac gcggcattgc ttatctgcat
     3241 atgtcagaac cagattgggc gggggggtga accgtatact gatgcgttcc gcgaaaaagt
     3301 acgcgcccgt ttccacggtc cgattatcgg cgcaggtgca tacacggtag aaaaagccga
     3361 aacgctgatc ggcaaagggt taattgatgc ggtggcattt ggtcgtgact ggattgcgaa
     3421 cccggatctg gtcgcccgct tgcagcgcaa agctgagctt aatccacagc gtgctgaaag
     3481 tttctatggc ggcggtgcgg aaggctacac ggattacccg acgctgtaat ccaacattgc
     3541 gagcggcgca aagccgccgc tatactaaaa caacattttg aatctgttag ccattttgag
     3601 gatataaaga tgcgtcttct tcataccatg ctgcgcgttg gcgatctgca acgctccatc
     3661 gatttttata ccaaagtgct gggcatgaaa ctgctgcgta ccagcgaaaa cccggaatac
     3721 aaatactcac tggcgtttgt tggctacggt ccggaaaccg aagaagcggt gattgaactg
     3781 acctacaact ggggcgtgga taaatacgaa ctcggcactg cttatggtca catcgcgctt
     3841 agcgttgata acgccgctga agcgtgcgaa aaaatccgtc aaaacggggg taacgtcacc
     3901 cgtgaagcgg gtccggtaaa aggcggtact acggttatcg cgtttgtgga agatccggac
     3961 ggttacaaaa ttgagttaat cgaagagaaa gacgccggtc gcggtctggg caactaatct
     4021 cctgccgggc gtgaactcat cgcgcccgca tctttactgc atcgacaagt aatatttgtc
     4081 ataatgcgcg ctgcaattta tccgtattaa gagaatcaga tgtccgataa cgctcaactt
     4141 accggtctgt gcgaccgttt tcgtggtttt tatcctgttg tgatcgatgt tgaaacagcc
     4201 ggatttaacg ccaaaaccga tgcgctgctt gagattgccg ccatcaccct gaaaatggat
     4261 gaacaaggct ggctgatgcc ggacaccaca ttacatttcc acgtcgaacc atttgtcggc
     4321 gcaaatttgc agccagaggc cctcgccttc aacggcattg acccgaacga tcccgatcgc
     4381 ggcgcggtca gcgaatacga ggcgctgcac gaaattttta aagttgtacg taaaggcatt
     4441 aaagcgagcg gctgtaaccg cgccattatg gtggcgcaca atgccaattt tgatcacagc
     4501 tttatgatgg ccgccgcaga acgcgcctcg ctgaaacgta acccgttcca ccctttcgcc
     4561 acttttgaca ctgctgcact tgccgggctg gcactcggac aaaccgtatt gtcaaaggct
     4621 tgccagaccg ctggcatgga cttcgacagc acccaggcgc actccgcgct gtacgacacc
     4681 gaacgcactg ctgtgctgtt ttgtgaaatc gtcaaccgct ggaaacgtct gggaggctgg
     4741 ccgctacctg ccgccgaaga ggtgtaatcg agtcgatgcc tgatgacatg caatgattca
     4801 ggcatctata gtgaggctat tccacgcatc atgcatgata ttcacgggga atagcgttaa
     4861 tggcagataa tctaaaccct tcatcgcccc tgccggacgt gttttcaccg gcgacccgcg
     4921 actggtttct tcgcgccttt aaacagccga ccgctgtcca gtcgcaaacc tggcatgtgg
     4981 cggcgcgagg cgaacatgcg ctggtgattg caccgactgg ctccgggaaa acgctggcag
     5041 catttctcta cgccctcgat cagctcttcc gcgaaggcgg cgaagatacc cgcgaggcgc
     5101 ataagcgtaa aacctcacgc atcctctata tttcaccgat caaagctctg ggcagcgacg
     5161 ttcagcgcaa cttgcagatc ccgttacagg gtattgccga tgaacggcgg cggcgcggcg
     5221 aaacggaagt caatcttcgc gtggggatcc gtactggcga tacgcctgca caggaacgca
     5281 gcaaactcac ccgtaatccg ccggatattc tgatcaccac acccgaatca ctctatctga
     5341 tgctgacctc ccgcgcgcgc gaaacgctac gcggcgtcga aacggtaatt attgatgaag
     5401 tccacgcggt agcaggcagt aaacgtggtg cgcatctggc gttaagtctg gagcggctcg
     5461 atgcgctgct ccacacctca gcacagcgaa ttggcctttc tgccactgtg cgttcagcca
     5521 gcgatgtggc agcatttctt ggtggcgatc gcccggttac ggtagtcaac ccgcccgcaa
     5581 tgcgtcatcc gcagatacgg attgtcgtac cggtcgccaa tatggatgat gtctcatcgg
     5641 tcgccagcgg cactggcgaa gacagccatg ccggccggga aggctccatc tggccatata
     5701 ttgaaacggg tatccttgat gaagtgttgc gccatcgctc gaccattgtc tttactaatt
     5761 cacgcgggct ggcggaaaaa ctgacggcac gattgaatga gctttacgcc gcacgcttac
     5821 agcgttcccc gtctatcgcc gttgatgcgg tccatttcga gtcgacctcc ggcgcaacct
     5881 ctaaccgtgt acaaaatagc gacattttta tttcccgctc acaccacggc tccgtctcta
     5941 aagaacaacg agcaatcacc gaacaggcgc tgaaatcggg tgaattacgc tgcgtggtcg
     6001 caacctccag tctcgaactg gggattgata tgggcgcggt ggatctggtg attcaggtgg
     6061 caacgccgct ttctgttgcc agtgggttac aacgcattgg acgtgccgga cacgaggtag
     6121 gtggtatctc caaagggctt tttttccccc gaacccggcg tgacttagtc gattccgcag
     6181 tcattgttga gtgtatgttc gcaggcaggc tggaaaacct gacaccaccg cataatccac
     6241 tcgacgtcct tgcgcagcaa accgttgccg ccgcggcgat ggatgcatta caggtagacg
     6301 aatggtactc ccgcgtacgc cgtgccgcac cgtggaaaga tctgccaaga cgtgtttttg
     6361 acgccacgct ggatatgctt tccgggcgct atccctctgg cgatttttct gcttttcgcc
     6421 ccaaactggt ctggaaccgg gagaccggga tattgaccgc ccgacctggc gctcagctgt
     6481 tggcggttac cagcggtggc accattccgg atcgtggcat gtatagcgtg ttattacccg
     6541 aaggtgaaga acaggccggt tcgcggcggg tgggtgaact ggatgaggag atggtatatg
     6601 agtcgcgggt gaacgacatt atcactctcg gcgctacctc atggcggatc cagcaaatca
     6661 cccgcgatca ggtgattgtg actcctgctc caggtcgttc tgcccgcctt cccttctggc
     6721 gtggtgaagg taacggacgt ccggtagaat taggcgagat gatcggcgat tttctccatt
     6781 tactggcgga tggcgcgttc ttttccggga ctattccccc gtggttggca gaagaaaata
     6841 cgatcgccaa tattcagggg ttgattgacg agcagcgcaa cgcgacgggc atcgttccgg
     6901 gtagtcgtca tcttgtcctc gaacggtgcc gtgatgaaat tggtgactgg cgtattattt
     6961 tgcactctcc ctatggaaga cgggtgcatg aaccctgggc gctggcgatt gccgggcgaa
     7021 tacatgcact atggggcgct gacgcgtctg tggttgccag tgatgacggc attgttgcgc
     7081 gcattcctga caccgatggt aaattacctg atgccgcgat ttttttgttt gaaccagaaa
     7141 cgttgctgca aattgtccgc gatgcggtag gcagctcggc acttttcgcc gcccgttttc
     7201 gcgaatgcgc cgcgcgggca ttattaatgc cgggccgcac gccgggccat cgcaccccgc
     7261 tctggcaaca acgactgcgc gccagtcagt tgctggaaat cgctcaggga tatccggatt
     7321 ttccggtcat tctcgaaacc ctacgcgaat gtctgcaaga tgtttatgat cttcccgcgc
     7381 tggaacgttt gatgcgtcgc ctgaacggtg gcgaaattca aatatccgat gtaacgacca
     7441 ctacgccctc gcctttcgcc gccagtttat tgttcggcta tgtcgcggaa tttatgtacc
     7501 agagcgacgc cccgctggca gagcgccggg catccgtact gtcgctggac agcgagttac
     7561 tgcgcaatct actcggacag gtcgaaccgg gggaattact cgacccgcag gtcattcgcc
     7621 aggtggaaga agagttgcaa cgactggcac ctggcagaag agcgaaaggt gaagaaggat
     7681 tgttcgacct gctgcgcgaa ctgggtccaa tgaccgttga agacctggcg cgacggcata
     7741 caggcagcag tgaagagatt gcgtcgtatc tggaaaatct tcttgcagta aaacgaatct
     7801 tcccgacgat gattagcggg caggagcgtc ttgcctgtat ggatgatgcc gccaggctgc
     7861 gcgatgccct cggcgtacga ctaccagagt cactgccaga gatttatttg caccgagtca
     7921 gttacccgct tcgcgacctc tttctgcgct atctccgggc tcatgctctg gtcacagctg
     7981 aacaactggc tcatgagttt agtctcggta ttgccattgt cgaagagcaa cttcagcaac
     8041 tgcgcgaaca ggggctggtg atgaatctgc aacaagacat ctgggtgagc gatgaagtat
     8101 ttcgtcgtct gcgtttgcgc tcgctgcaag ccgccagaga agcgacacgt cccgttgcgg
     8161 ccacgaccta tgcgcgattg ctgctggaac gtcagggcgt attacccgcc accgatggta
     8221 gcccggcact ctttgcctca acatcgccag gcgtttatga gggcgtggat ggcgtcatgc
     8281 gggtgatcga acagcttgca ggagtcggtt tacccgcctc actctgggaa agccagatcc
     8341 tgcctgcccg cgtacgcgac tattcgccag aaatgctcga tgaattactg gcaaccggtg
     8401 cggttatctg gtcggggcaa aaaaagctgg gtgaagatga cggtctggtg gcactgcatc
     8461 tacaggaata tgccgcagaa tcgttcactt ccgccgaagc gaatcaggcg aatcgttcgg
     8521 cactgcagca agcgatagtc acggttctgg ctgacggagg agcctggttt gcacaacaaa
     8581 tcagccaacg gatacgcgat aaaatcggcg aatcggttga tccctcagcc atgcaagagg
     8641 cgttatgggc gctggtctgg caaggcgtca tcaccagcga catttgggca ccgttacgcg
     8701 ccctcaccca cagcagtacc aacacacgca cctcaactcg ccgcagtcac cgggctcgtc
     8761 gtggacgtcc tgtctatgcg caacccgtct cgccgcgggt atcttacaac acaccaaagc
     8821 ttgctggacg ctggtcgtta ttgcaggtgg agccactaaa cgataccgaa aggatgctgg
     8881 cgctggcgga aaatatgctc gaccgctacg gcatcatcag tcgtcaggcg gtgatagccg
     8941 aaaatatccc tggtgggttt ccatcgatgc aaacgctttg tcgaagtatg gaagactccg
     9001 ggcgaattat gcgaggtcgt tttgtagaag gtctgggtgg cgcgcaattc gctgaacgtc
     9061 tgactattga ccgattgcgc gatctggcga cacaaaccgc gcaaacgcgc cactatacac
     9121 cagtggcgct ctccgccaac gatccggcta atgtgtgggg aaatcttctg ccctggcctg
     9181 cacatccggc aacgctgata ccaacgcgtc gggcgggtgc gctggtggtc gtttctggcg
     9241 gcaaattgtt actctatctg gcgcaaggtg gcaaaaaaat gctggtctgg caggaaaaag
     9301 aggaattact cgccccagag attttccacg cgctgactac cgcactgcgt cgcgaaccac
     9361 ggctgcgctt tacgctaaca gaagtgaatg acctaccggt ccggcaaacg ccgatgttta
     9421 cgctgctgcg tgaggcggga ttttcaagtt cgccacaagg actggattgg ggatagagaa
     9481 aggactgacg gatgcccgtt cgcatccgtc agtattgcag gacggattat tcagcgtccg
     9541 gctcttcaga cttgtattta gcggcagttt ctttgatcag ctgctgcagt tcgccacgct
     9601 gatacatttc gatcacgata tcacaaccgc cgaccagctc gccgtcaacc cacagttgcg
     9661 ggaaggtcgg ccagttagca tatttcggca gttccgcacg aatgtccgga ttctgcagaa
     9721 tatcaacata ggcaaaacgt tcgccacatg cggcaagcgc ctggactgcc tgggcagaga
     9781 aaccgcagct cggcagtttc ggtgaacctt tcatgtacag caggatcggg ttttcagcaa
     9841 tctggcgttg gattttttcg atagtggtgc tcattgtctt gcttcctcaa acttctttac
     9901 ggcagtaatc tgacattgta gcgggtcagt gcggcatcgg aaaataacat tttcatcacg
     9961 cttttgctat tttatccctt tgctctattt gttgcatttc aaatattcgt ttcttccctg
    10021 acgcacggca aactggcgta tttataacca tttttagctt tcactgctgc atttttttgc
    10081 gctcgccaac gaaacgtatt ttttaacaat aaaagctatt aactttctct tcttctatgc
    10141 attagaatca tcaagttttg taaatcagac gcaggcatga tagacctgcc ttaacagagg
    10201 gacgctcagt ggcgcggata aaccgtattt cgatcacgct ctgtgcttta ctttttacca
    10261 ccctgccttt aacgcctatg gcccatgctt caaagcaagc cagggagagt tctgctacta
    10321 ctcatatcac caaaaaagca gataaaaaga aaagcacggc aaccaccaaa aagacccaga
    10381 aagccagcca gaaaacagcg aaaaaagccg ccagtaaaag tacgatcaaa agcaaaaccg
    10441 cttcttccgt taaaaaatct tccattaccg cttctaaaaa cgccaaaact cgcagcaaac
    10501 acgccgtcaa taaaacggcc tcagccagct ttaccgaaaa gtgtaccaaa cgtaagggct
    10561 ataaatcgca ttgtgtgaaa gtcaaaaatg ccgcgtcagg aactcttgcc gacgcgcaca
    10621 aagcgaaggt gcaaaaagcc acgaaagtgg caatgaataa actgatgcag caaattggta
    10681 agccatatcg ttggggtggc agctcaccgc gtaccggttt tgattgcagc ggcctggttt
    10741 attacgctta taaagatttg gtgaaaatac gtattccgcg tacggcgaat gaaatgtatc
    10801 acctgcgaga tgcagcgcca atcgaacgta gtgaactgaa aaacggcgac ctggtctttt
    10861 tccgtactca gggacgcggt acagccgatc atgtcggcgt atatgtcggc aacggcaaat
    10921 ttattcagtc accgcgtacg ggtcaggaaa ttcaaatcac ttctctcagt gaagactact
    10981 ggcagcgcca ctatgttggc gctcgccggg taatgacccc aaaaacactt cgctaaaact
    11041 ttaccctgtt gttacggcaa cagggtaagt tcatcttttg tctcaccttt taatttgcta
    11101 ccctatccat acgcacaata aggctattgt acgtatgcaa attaataata aaggagagta
    11161 gcaatgtcat tcgaattacc tgcactacca tatgctaaag atgctctggc accgcacatt
    11221 tctgcggaaa ccatcgagta tcactatggc aagcatcatc agacttatgt cactaacctg
    11281 aacaacctga ttaaaggtac cgcgtttgaa ggtaaatcac tggaagagat tattcgcagc
    11341 tctgaaggtg gcgtattcaa caacgcagct caggtctgga accatacttt ctactggaac
    11401 tgcctggcac cgaacgcggg tggcgaaccg actggaaaag tcgctgaagc tatcgccgca
    11461 tcttttggca gctttgccga tttcaaagca cagtttactg atgcagcaat caaaaacttt
    11521 ggttctggct ggacctggct ggtgaaaaac agcgatggca aactggctat cgtttcaacc
    11581 tctaacgcgg gtactccgct gaccaccgat gcgactccgc tgctgaccgt tgatgtctgg
    11641 gaacacgctt attacatcga ctatcgcaat gcacgtcctg gctatctgga gcacttctgg
    11701 gcgctggtga actgggaatt cgtagcaaaa aatctcgctg cataataact gatggcaaat
    11761 gcaacattgc ctgatgcgct tcgcttacca tgccaactcg gatgaagcgt taacaccgca
    11821 tccggcaaga taaatcgcac gttgccagca actgtaacgc agaaggttat ccttctgcgt
    11881 ttttgtttaa ttagctgttg gcaacgcaaa ctgcctcagg ttgttttctg gctgacataa
    11941 acaccagcaa taatgccagt cccgcgacaa tcgcccccat caccggcaca aagctgtatc
    12001 ccagcccagc ggaaattacc gcaccaccag cagctgctcc cagcgcattt ccaagattaa
    12061 aggcaccaat attgactgat gaagacagac ctggcgcttc actggcgaca cgcatcacgc
    12121 gcatctgtaa cggcggtacg accgcaaagg ttgcagctcc ccacaccacc atgctaatag
    12181 ctgcgccgaa ctcattgcgg gccaggaacg ggattgccag cataatcacc atcagcagca
    12241 acaaaaagcc tttcaacgtg ccattaactg aacgatctgc cagtttgccg ccgagatagt
    12301 taccgataga gaatccgaca ccaatcagca ccagctttgc cgtgacgaac accggcgttg
    12361 cgtgggtaat gctttgcagt accggagaga tataggtgta gagggtaaac attgcaccag
    12421 ctcccagtac cgtcgtcagc aatgcagaca gcacctgcgg acgcattaat accgccagct
    12481 cttttttcac ttcaggtcgc gcccctgcgc cacctttagg taatgagaag aacagactta
    12541 ccattgaaat cacgcccagc accgccgttg ccagaaatga catccgccag ccgatggttt
    12601 cgcccaacca ggtcgctgcc ggaacgccac cgatatttgc cagggttaac cccataaaca
    12661 tagtggcaac tgcgctggcc tgtttatgtt ttggcaccac gcttgcggcc acaactgaac
    12721 ccaaaccaaa aaatgctccg tgattcaggc tggtcaaaat gcgtgaaagc atgagagtca
    12781 tataatccgg cgcgatggcg gaaagtacgt tgccgagcgt gaaaatcgcc atcaggaaaa
    12841 tcaacgcact gcggcgagca cgatgagaaa gtagaagcgt catcagcggc gcgccaacca
    12901 ttacgccaac tgcataggca ctgattaaca ttccggcagc gggaatcgag acatccacac
    12961 cgcgcgcaat gacgggcaat aagcccattg gcgagaactc cgttgtccca ataccaaacg
    13021 cgccaatcgc cagcgccagc aacggatagt taattttcat gccttatctc cacctcttcg
    13081 cgtcattacg cgatattcat taaagtggcg aaagcatgac agcaatcaca aaaaaatgaa
    13141 aataacaaaa agagaaaaca cttttgccat tttgctaaca aacaggaagg agatgcgagg
    13201 gagaacgcgc tccctcgaga ggaaatcagt gcagcgcggc agtcaaaccc acggccacga
    13261 tcaaaccgag gacgataatc gttgttacca gtgaaaattt aaggtcggtg ctcatcaagt
    13321 tttctccttt tttattacca cacaaaaagt gatattacgc atttttacac actgtgatga
    13381 aaaaatctcc cgtcatttat aatgataagt gtttttacca cttccccttt tcgtcaagat
    13441 cggccaaaat tccacgctta cactatttgc gtactggcca ttgacccctt cctgacgctc
    13501 cgtgtcgttt ttccggcgta ccgcaacact ttggttgtgc gtaaggtgtg taaaggcaaa
    13561 cgtttacctt gcgattttgc aggagctgaa gttagggtct ggagtgaaat ggaatggcaa
    13621 caataaaaga tgtagcgaaa cgagcaaacg tttccactac aactgtgtca cacgtgatca
    13681 acaaaacacg tttcgtcgct gaagaaacgc gcaacgccgt gtgggcagcg attaaagaat
    13741 tacactactc ccccagcgcg gtggcgcgta gcctgaaggt taaccacacc aagtctatcg
    13801 gtttgctggc gaccagcagc gaagcggcct attttgccga gatcattgaa gcggttgaaa
    13861 aaaattgctt ccagaaaggt tacaccctga ttctgggcaa tgcgtggaac aatcttgaga
    13921 aacagcgggc ttatctgtcg atgatggcgc aaaaacgcgt cgatggtctg ctggtgatgt
    13981 gttctgagta cccagagccg ttgctggcga tgctggaaga gtatcgccat atcccaatgg
    14041 tggtgatgga ctggggtgaa gcaaaagctg acttcaccga tgcggtcatt gataacgcgt
    14101 tcgaaggcgg ttacatggcc gggcgttatc tgatagaacg cggtcaccgc gaaatcggcg
    14161 ttatccccgg cccgctggaa cgtaacactg gcgcaggccg ccttgccggt tttatgaagg
    14221 cgatggaaga agcgatgatc aaggtgccgg aaagctggat tgtgcagggt gactttgaac
    14281 ctgaatccgg ttatcgcgcc atgcagcaaa tcctgtcgca gccgcatcgc cctactgccg
    14341 tcttctgtgg tggcgatatc atggcaatgg gcgcactttg tgctgctgat gaaatgggtc
    14401 tgcgcgtccc gcaggatgtt tcgctgatcg ggtatgataa cgtgcgcaac gcccgctatt
    14461 ttacgccggc gctgaccacg atccaccagc caaaagattc gctgggtgaa actgcgttca
    14521 acatgctgtt ggatcgtatc gtcaacaaac gtgaagaacc gcagtccatt gaagtgcatc
    14581 cgcgcttgat tgaacgccgc tccgtggctg acggcccgtt ccgcgactat cgtcgttaat
    14641 cacccgttgc gggagtctct tccggctccc gcagccactc cttattcagc gtctcactat
    14701 cgccgagata ctcaagcaac caggttaacg caggcgacat atcattttgc tgccatgtca
    14761 gacaacatgc cgaatccggg aaggggtttt ccagttctag tgctacccac ttaccctcat
    14821 taagccacgg tttggcgaaa tgtgttggca ccatccctat gcataatcct gccgagatac
    14881 aggttgccga tgattcccag tcaggcacga caagtctttt ttggttatcc agcaaccagg
    14941 taatacgttt aggtagcgtt cgcgaggtgt cttcgcgcac caacgacggc cagttgcgca
    15001 acgtatcatc gctgaacggg ccatccatca acgccagcgg gtggtgactg gcaacaacgc
    15061 aactccagct tagcatcccc atatcccgga aggcataacg accgcctacc ggaatcgcgc
    15121 gtgttgcgcc aatcgccagt tccacgcgcc cgtcggaaag cgcatcccag acaccgttga
    15181 acacttcctg aaagacaaga agttcgacat catcaaaatg gcgataaaaa tcaacgatca
    15241 tctgccgtgt acgttctggc ctgacaatat tatccactgc gatagctaac tgaccgcgcc
    15301 agccgttcgc tatctgctga cattgctgcc gggtgatctg catttttttg acaacagagc
    15361 gcccttcttt gagaaaccac gccccggcag cggtcagttc cacatcacgg tgacgtcgtt
    15421 caaacagcgg caccgccagc cactcttcca gctgacgcac ggtatagctg accgcagaag
    15481 gaacgcgatg caactcctgt gccgcagcgc taaaactacc attacgcgct accgcatcaa
    15541 caacttcgag tgaatattct gaccacatag tctgcctgca aaatttttga aaccagtcat
    15601 caaatattac cgtttcacaa cactaatttc actccctaca ctttgcggcg gtgtttaatt
    15661 gagagattta gagaatatac atgcaacctg ggaaaagatt tttagtctgg ctggcgggtt
    15721 tgagcgtact cggttttctg gcaaccgata tgtatctgcc tgctttcgcc gccatacagg
    15781 ccgacctgca aacgcctgcg tctgctgtca gtgccagcct tagtctgttc cttgccggtt
    15841 ttgccgcagc ccagcttctg tgggggccgc tctccgaccg ttatggtcgt aaaccggtat
    15901 tattaatcgg cctgacaatt tttgcgttag gtagtctggg gatgctgtgg gtagaaaacg
    15961 ccgctacgct gctggtattg cgttttgtac aggctgtggg tgtctgcgcc gcggcggtta
    16021 tctggcaagc gttagtgacg gattattatc cttcacagaa agttaaccgt atttttgcga
    16081 ccatcatgcc gctggtgggt ctatctccgg cactggctcc tctgttagga agctggctgc
    16141 tggtccattt ttcctggcag gcgattttcg ccaccctgtt tgccattacc gtggtgctga
    16201 ttctgcctat tttctggctc aaacccacga cgaaggcccg taacaatagt caggatggtc
    16261 tgacctttac cgacctgcta cgttctaaaa cctatcgcgg caacgtgctg atatatgcgg
    16321 cctgttcagc cagttttttt gcatggctga ccggctcacc gttcatcctt agtgaaatgg
    16381 gctacagccc ggcagttatt ggtttaagtt atgtcccgca aactatcgcg tttctgattg
    16441 gtggttatgg ctgtcgcgcc gcactgcaga aatggcaagg caagcagtta ttaccgtggt
    16501 tgctggtact gtttgctgtc agcgtcattg cgacctgggc tgcgggcttc attagccatg
    16561 tgtcgctagt cgaaatcctg atcccattct gtgtgatggc gattgccaat ggcgcgatct
    16621 acccgattgt tgtcgcccag gcgctgcgtc ccttcccaca tgcaactggt cgcgccgcag
    16681 cgttgcagaa cactctacaa ctgggtctgt gcttcctcgc aagtctggta gtttcctggc
    16741 ttatcagtat cagcacgcca ttgctcacca ccaccagcgt gatgttatca acagtagtgc
    16801 tggttgctct gggttacatg atgcaacgtt gtgaagaagt agactgccag aatcatggca
    16861 atgccgaagt cgctcatagc gaatcacact gatctatatc gatatgctta tacttaggct
    16921 gctaacaaaa ttttgttgta tgattgaaat tagcggccta tactaatttc gagttgttaa
    16981 agctacgata aatattatgt ttttacgggg acaggatcgt tcccgactcg ctatggatgg
    17041 tcatttcggc aagggttcct cctttccctc tgttctacgt cggattatag actcgcggtt
    17101 ttttctgcga gatttctcac aaagcccaaa aagcgtctac gctgttttaa ggttctgatc
    17161 accgaccagt gatggagaaa ctatgagttc atcgtgtata gaagaagtca gtgtaccgga
    17221 tgacaactgg taccgtatcg ccaacgaatt acttagccgt gccggtatag ccattaacgg
    17281 ttctgccccg gcggatattc atgtgaaaaa ccccgatttt tttaaacgcg ttctgcagga
    17341 aggctctttg gggttaggcg aaagttatat ggatggctgg tgggaatgtg accgactgga
    17401 tatgtttttt agcaaagtct tacgcgcagg tctcgagaac caactccccc atcatttcaa
    17461 agacacgctg cgtattgccg gcgctcgtct cttcaatctt cagagtaaaa aacgtgcctg
    17521 gatagtcggc aaagagcatt acgatttggg taatgacttg ttcagccgca tgcttgatcc
    17581 cttcatgcaa tattcctgcg cttactggaa agatgccgat aatctggaat ctgcccagca
    17641 ggcgaagctc aaaatgattt gtgaaaaatt gcagttaaaa ccagggatgc gcgtactgga
    17701 tattggctgc ggctggggcg gactggcgca ctacatggca tctaattatg acgtaagcgt
    17761 ggttggcgtc accatttctg ccgaacagca aaaaatggct caggaacgct gtgaaggcct
    17821 ggatgtcacc attttgctgc aagattatcg tgacctgaac gaccagtttg atcgtattgt
    17881 ttctgtgggg atgttcgagc acgtcggacc gaaaaattac gatacctatt ttgcggtggt
    17941 ggatcgtaat ttgaaaccgg aaggcatatt cctgctccat actatcggtt cgaaaaaaac
    18001 cgatctgaat gttgatccct ggattaataa atatattttt ccgaacggtt gcctgccctc
    18061 tgtacgccag attgctcagt ccagcgaacc ccactttgtg atggaagact ggcataactt
    18121 cggtgctgat tacgatacta cgttgatggc gtggtatgaa cgattcctcg ccgcatggcc
    18181 agaaattgcg gataactaca gtgaacgctt taaacggatg tttacctatt atctgaatgc
    18241 ctgtgcgggt gctttccgcg cccgtgatat tcagctctgg caggtcgtgt tctcacgcgg
    18301 tgttgaaaac ggccttcgag tggctcgcta aaggctattc tatcgccccc tctccggggg
    18361 cgatttcaga tcaggcttct gtacctggtt gattcatggc attttctcgt gccgccagca
    18421 ctcgttctac cgtatctacc actgcctgag tttgtggatc gatttcaatg ttgacgcgtg
    18481 cgccaagttt tttcttccca agagtcgtgc gttccagtgt ttccggaatt aaatggacgc
    18541 aaaaacgcgt tggcgtgact tcaccgacgg tcaggctaat accgtcgatg ccaataaatc
    18601 ctttgtacag aatatatttc atcaactgac tatcctggac tttaaaccag atctggcgat
    18661 tattttccga ggttaatatt ttcgccactt cagcggtggt cataatatga cctgacatta
    18721 agtgtccgcc aatttcatca ctgaatttcg ccgcacgctc aacgtttacc caatccccca
    18781 cttttaaatc gccaagattg gtaatgcgta acgtttcttt catcaggtca aaactgacat
    18841 ggttgccgtt aatttccgtc acggtcaggc agcaaccgtt atgcgccacg gaagcaccgg
    18901 tttccagacc gtccagcatg tggtcgggta actccaccac atgtgtgcga aaatttggtt
    18961 tctcgtcaat cgacaccagt tttgcggtgc cctgtacaat ccccgtaaac atacttacaa
    19021 ctcctgaaat cagttaagac attctgttca gcacaatagc aggtggaaaa cgcccttacc
    19081 agtgaagggg taagaatgac tattttttca ctggagaatt aataaatcct cgctacaata
    19141 gactgaattt cccctgcttc ttctttttgc tgcccattca ggcggctttt tagtctctca
    19201 tataactaca aataaaaggt gttcacgtgc agaagtatat cagtgaagcg cgtctgttat
    19261 tagcattagc aatcccggtg attctcgcgc aaatcgccca aactgcgatg ggttttgtcg
    19321 ataccgtgat ggcgggcggc tatagtgcca ccgacatggc ggcggttgct atcggaactt
    19381 ctatctggct tccggcgatc ctctttggtc acggactgct gctggcatta acgccggtta
    19441 tcgcgcaatt aaatggttcc ggtcgacgtg agcgcattgc gcatcaggtg cgacaaggtt
    19501 tctggctggc aggttttgtt tccgttctca ttatgctggt actgtggaat gcgggttaca
    19561 ttatccgttc catgcaaaac atcgatccgg ctctggcgga caaagccgtg ggttatctgc
    19621 gtgcgttgct atggggcgcg ccgggctatc ttttcttcca ggttgcccgt aaccagtgtg
    19681 aaggtctggc aaaaaccaag ccgggtatgg tgatgggctt tatcggcctg ctggtgaaca
    19741 ttccggtgaa ctatatcttt atttacggtc atttcggtat gcctgagctc ggtggcgttg
    19801 gttgtggcgt ggctactgct gcggtgtatt gggtcatgtt ccttgccatg gtttcttaca
    19861 ttaaacgcgc ccgctcgatg cgcgatattc gtaacgaaaa aggcaccgct aaacccgatc
    19921 ctgcggttat gaaacgactg attcaactcg gtttgccaat tgcgctggcg ctgttctttg
    19981 aagtaacact gtttgccgtc gtggctctgt tagtgtctcc gctaggtatt gttgatgtcg
    20041 caggacatca gattgccctg aactttagtt cactgatgtt cgtacttccc atgtcgctgg
    20101 cggcagcggt aaccatccgc gtaggttatc gtctgggtca gggctcaacg ctggatgcgc
    20161 aaaccgctgc gcggaccggg cttatggtgg gtgtctgtat ggcaaccctg acggccattt
    20221 tcacggtttc actgcgggag caaatcgccc tgttgtacaa cgacaatccc gaggttgtaa
    20281 cgctggctgc gcatttgatg ttgctggcgg cggtatatca gatttctgac tcaatccagg
    20341 tcattggcag tgggattttg cgtggatata aagatacgcg ttccattttc tatattacct
    20401 ttacggctta ctgggtgctg ggcttgccaa gcggctatat tctggcactg accgatctgg
    20461 tcgttgaacc tatggggcca gcaggcttct ggataggctt tattattggc ctgacgtcgg
    20521 cagccattat gatgatgctg cgtatgcggt tcctgcaacg tctgccgtca gccatcattc
    20581 tgcaacgagc agctcgctag taaagacaag gcgcaacctt cacgggttgc gcctgtgttt
    20641 ttacgcaggc tggagcgttg cgccaatccc gtcttcgtct ggctgtagtt tcagtgcgtt
    20701 acacagagtt tgccgactgg catctgttat ctcaacgtgt atatccccgg taacttcccc
    20761 tttcacattg atgaagtcag gtgaaatttc tttcgcactg gtttgcaggc aaatggctcc
    20821 ctctccacgc aggcgtttca ctttcagttg cacatgcgtt gccgcaccag tgattagcca
    20881 cagttttccg ccattgagat tgatgttacc aacactgaaa gtgacactgt caccttttac
    20941 cgtactgccg tcgagaatca atacaccgcc ctgctgtaca gtaatcgttg attgttgcag
    21001 atctgcgcca tagccaataa tcattgaagc gccgtccagt aatgcaacac taccggtcag
    21061 ttcaaccggt gcgcgatctt cctctacgac aaaattttcg tccgcgctgg tgggtgacaa
    21121 caggatcact gtcccacctt gtattaccgc attttccagc gtaccggcat gcacttcagc
    21181 ccgccccccc acattaacgg acaggttttg cgtcttaccg gaactgtaga gctgaagacc
    21241 atccgtaccc agacgataga tgcctccatt ctcaacaata gtgtcagagt cttcaccacc
    21301 gagattattc atggtgccac cagcaagcaa tgtatcactg gctttccccg ccagcataaa
    21361 aacgccgccc tgattaatct cacaattgct ggcttcccca cctggataca ccaacaattc
    21421 accaccttca tctaccacga ccgctgaggt tgtcccattg accaacaggc caccctcttg
    21481 atcgagaata attttttccg cgcgatggcc ttccagtaca cgcaggttac cgccattttc
    21541 cagcaacaaa ccgcaggcat aacctttatc aacgctgaat tcaccttcgg gatggcggcc
    21601 attaaccgta gcgagcgtag agagactaat tgcgcctccc tcggactgaa caatatctgt
    21661 ggcaatggca ccgtcgctga ccgatagcgt gccattgttg ccgtactgtg tgcatttggc
    21721 gatcccaccg gttgccactt tcaaccagcc agcccccgtg accacggtat tttcggctct
    21781 tccgccagct tcaacaatta actcaccttc agtaagttgc gtcgcgccaa tgacctcgcc
    21841 ggtcttaaca atttgcacgt tcccgtcgct catcaagttt ttcgcatcag atcccatgat
    21901 ttattccttt gctgcatctg tgtgccttta ttgctaccta agtgtaaagg ctacggagga
    21961 tttatccacg acagattttg agatggtggc aaacaactct gtttaaactc tgatacacgg
    22021 attattgggt tgtatctgat gtaaatgcga tcctgaataa aaatcgccct tgcaaatcaa
    22081 caaaatatag ccaatttgct taaaagctct gcaaacggtc gaaatagcgc agaaaattac
    22141 gttttgcctc ttgccacctt cccactctgc cgctaatatt cgtccccgtt gtcacctaca
    22201 acgttgcgtt catagctcag ttggttagag caccaccttg acatggtggg ggtcgttggt
    22261 tcgagtccaa ttgaacgcac catcctgcgt ccgtagctca gttggttaga gcaccacctt
    22321 gacatggtgg gggtcggtgg ttcgagtcca ctcggacgca ccagattttc ttaatctggt
    22381 cttctccttt tttccctctg tttcttctct gtatccaata cgttaaaaga tttacactgt
    22441 cttcgtatgc gttatcagaa ggagaatcgc tatggcaact ttgttacaac ttcattttgc
    22501 ttttaatggc ccatttggcg acgcaatggc tgagcagctt aaaccgcttg ctgagtcgat
    22561 taatcaggaa cctggttttc tgtggaaggt atggacagaa agtgaaaaga accacgaagc
    22621 cggtgggatc taccttttca ctgatgaaaa aagcgccctt gcctatctgg aaaaacacac
    22681 tgcccgactg aaaaatctcg gcgttgagga agttgtcgcc aaagttttcg atgtcaatga
    22741 gccactgagt caaatcaatc aggcaaaact cgcctgacag attttaatca agggcggtta
    22801 gcgccctttt catccctgtc tgaaatttct caaattctaa aaatttcaac caaacttatc
    22861 tgataacact aaattcgaaa gaatgcgtac aggtaagtaa caatgaaaaa aattgctatt
    22921 gtgggtgccg ggcctacggg gatctacacc ttattctcgc ttctgcagca acaaactcca
    22981 ctttctattt ctatcttcga gcaggctgac gaagccggtg tcgggatgcc atacagtgat
    23041 gaggaaaact caaaactgat gctggcaaat attgccagta ttgaaatacc gccgatttat
    23101 tgtacgtatc tcgaatggct acaaaagcaa gaagccagcc atctccagcg ttatggcgtc
    23161 aaaaaagaaa ccttgcacga tcgtcagttt ttaccgcgaa ttctgctggg cgaatatttc
    23221 cgcgatcaat ttttacgatt agtagaccag gcacgacagc aaaaatttgc ggtggctgtt
    23281 tatgaatcat gcctggttac cgatctgcaa attacaaatg ctggcgtcat gctcgctaca
    23341 aatcaggatt tacccagcga gacgtttgat ttagcagtga tcgccacggg tcacgtctgg
    23401 cctgatgaag aaaaagtaac cagaacgtac tttcccagcc cgtggtcagg cctgatggaa
    23461 gcaaaggtcg atgcgtgtaa cgtgggtatt atgggaacat ccttgagcgg actggatgcg
    23521 gcaatggcag tggctattca gcatggttcg ttcattgaag atgataaaca acacgtcatt
    23581 tttcaccgcg ataacgcaag tgaaaagcta aatattacgt taatgtcgcg cacaggtatt
    23641 ttacccgaag ccgatttcta ttgccctatt ccctacgagc ccttacacat cgtcactgat
    23701 caggcattaa atgctgagat tcaaaaaggc gaatatggtc ttttggatcg ggtatttaga
    23761 ttgatagtag aggaaatcaa gtttgctgat ccaggctgga gccaacgcat agccttagag
    23821 agcctgaatg tcgattcctt tgctcaagcc tggtttgccg agcgcaaaca acgcgaccca
    23881 ttcgactggg cagaaaaaaa tctccaggaa gtcgaacgca ataaacgaga aaaacatact
    23941 gttccctggc gttatgtcat tctgcgcctg catgaagcag tacaggaaat tgttccacat
    24001 ctgaatgaac acgaccataa acggttcagt aaaggccttg cccgggtttt catcgataat
    24061 tatgcggcaa tcccttcaga gtctattcgt cgactactgg ccttacgtga agcgggaatc
    24121 attcatattc tcgccctcgg tgaagactac gaaatggaaa ttaatgagtc gcgcaccgtc
    24181 ctgaaaacgg aagacaacag ctactcgttt gacgttttta ttgatgcccg tgggcagcgt
    24241 ccgcttaaag tgaaagatat tcctttccct ggactacgcg aacaattaca gaaaacaggg
    24301 gataaaaacc ctgatgttgg cgaagattat acgttacagc aacccgaaga tattcgtggg
    24361 cgcgtagcgt tcggcgcgtt gccctggtta atgcacgacc agcctttcgt tcagggactt
    24421 acggcatgtg cagaaattgg tgaggcgatg gctcgggctg tcgtaaaacc tgcctcccgt
    24481 gcacgtaggc gtctttcgtt tgattaaagg taaagctatc agcactcaac aaactgatgc
    24541 tgttcattaa gacgatagcg gactccggct cgaatattgt tttcaccttc aatggcgacg
    24601 gcgaagcgaa ctctttcgcc gtcatgatat gccagtgcgg cgcttccccc tggcccgaga
    24661 ataattgagt cgaccacgcc cgtgctcact attgttgaat cttcaccgct ggcaatgatg
    24721 tgtacattgt cgccgctgtt ggcaattctg gcgtttgcgc caaagctggc gatttgcacc
    24781 aggtcaccat tactggcgac atgacaccgt tcacccaatg tacaaacgcg tactcgcatt
    24841 ccggtattcg caatccgact actatctcca gcgctgctta tccgcgaaga attaccggtt
    24901 acagcaaccc gtgcgcgttc accggagctg ccaatgtgac tgttaaagcc gacacttcca
    24961 atccgcacgc tataacccat actggcaatt tgcgcggcat acccagcggt tgcgattctg
    25021 gcattatcct cacaacagcc gatgcgagca tgatcgtcgc tgcgggcaaa ctgactgcaa
    25081 aatgctctct ctccgggatc tgtggccagt ttttgcatag aagaaacttc ctgatgcgcg
    25141 aagttttcat ccgccagcca ttttttccac gcatactcaa ccagactctc cgcccagtcc
    25201 gtatatcctt cctcaatcag tgcatgatga atatcggcat aactaccacc gcgtgggaaa
    25261 tggcgcagga accagcgata catcaccgct ccgatacgcc attctcgcag gtctgctcgt
    25321 gtaatgatca ttcgttagtg ccggtgatag ccatcgacca tgcttttaaa ggtttcgtgt
    25381 ggcgtacgcc ccgtggtgca aagataaaga tgaccgaaga taaaaaagag gcttataaat
    25441 gccagagcaa aatgcgcctg caataaccag tatcttacgc caggaaacac atctcccacg
    25501 gcttgcggat agagacacag cagccccgtc aatagtaaca acggcagcaa tccatacatg
    25561 acaccaatat aggcaacctg ctgtaaggga ttaaatttag actgggttgt tgccgggaaa
    25621 ggatgttctt ccccctgcat aatgccaaac aaataaaatc gcgtttgttt tgccgctcgc
    25681 tccagccacc cctgacgacg aatgcgatag tggtgaccat tacccccaac ggcattgatc
    25741 agaacaaagc cgagccaaca tgccagtaac aaaaatccgc aaacttcatg caccgcaacc
    25801 agacttttaa ccgcagttgc gcccaccagc gcaaagtgat ttatcagccc acttgccagc
    25861 aacaatacaa agagtaacgc attcgaccag tgccataatc tgaccgcttt gctgtaaaga
    25921 taaaccttct caccatggtc ggtggctgat ttcttcacgc cacgagcacg aagcaaggca
    25981 tgcagcccca gcaccaacca catcccaacc agcaatagtc cggcaatgtt caaccacacc
    26041 ggcccaaatt cgggagtgaa ttgtggcaca tagttcgcca actggctctg aaactgttca
    26101 gcatgttgcg acgggttcat acattttcct ttttaatcaa atgttgaccg aaccggcgat
    26161 acaggtgcgg tttcccggca ccaggtagct gatattgata gtatttattt tcctgcaacc
    26221 acgcctgaat ttctgggcta tcttcgcgac caaagatcaa tgcgtgttcc ggacaggcgc
    26281 tgacgcaaat gggcggaaag ccttttgcca gtcgggactc agcgcagaaa tcacatttat
    26341 ctgccacttt agtgacaggg ttaaggtagc gaacctggta aggacaagcg ccaatgcaat
    26401 aactgcagcc gatgcactgc gatttttcta cccgcacgat cccctgctca tcacgccacg
    26461 acgcccctgt cgggcatacg tcgatgcaag gagcatcttc acagtgctgg catgactggc
    26521 gaaaatagtg atactgcgtt tcgttgtcgt tatcggtgac tggaatgtgt gctatcgata
    26581 aacggcttcc ctgagcgggg acgtggttag ttttacggca tgcgcgggcg caaatattac
    26641 agccattgca ccgtgactcg tcatgaatca tggcataccg aactttcttt tcttgcctcg
    26701 tgttcgctaa cagagaagac gcagaaccag taaaaaagat tactgttccc atccccagaa
    26761 caaatttgcg tcgagtgaag gacatctgat tatcccctaa tgcagtggct gcaactgtcc
    26821 accctatcca tgacatctcc ctgaaatttc aggggttctg aaactgctct acctcgaacc
    26881 attcgcataa accatacgcc ttgccatagg tcataaatcg ctcgctaaac aagtacaaca
    26941 ccgccgtcga tgactccacc tgccgtatat cagcatattc aggtgcaata tccatcgcgg
    27001 cgatcgcgga ttcgatctgc gcttcctgga agtaatatgg cgcttgcatc agcatggcga
    27061 ctttgtaagg acgaggatac gtctggcact caaaacgaac ggcgtgtgca atggcgcgac
    27121 aaatgtcctg ctccacaact tgcaaagaca tcgccgcgta gttttcactc atggcctgag
    27181 ttgagtaata gtaatcatcc tggcttcccg tcaaacaagc gatatccgcg tggtctccgt
    27241 tctgcttaat gctttccagc aaaactgaca gttcctcttc tgcaaccgaa tagggtggcg
    27301 agagaaacac ggcgcgcgcc accagccgcc cagccgcaga atgctgacga atgaactctg
    27361 ccaggatttc agcaggttct ggttcggtta ccgtatcaac ggggtgacgc atcccctgca
    27421 accattgccg cttcgcctcg tcaatttcgc tgacttccgc taacggcaat tcatcctgat
    27481 ggttcatctc acccattcct tacgcaggca gtagattgtg tgctgccaga tcggcggcaa
    27541 tatcttccag ccccagacgc tgcaatgttt cacgggtcgg acaaccaagc tgtgggtccc
    27601 agcccatttc tttgtagaac atcgtgagcg aagcatgcat atcgtcacgg tccattttgt
    27661 ccgtaccttc agtaaagacc gggatctgag gatccttgtc gaatacccag gaacagataa
    27721 gatcgtgttc attacgcata tctttggttt gcatcagttt taccgtgtag gcgcgatgca
    27781 acgtaaagat acgctctgca gctaaatcta atttttcctg agtcatctct tcgccagtga
    27841 tcgctttgaa gaatttggct tcaagcgcca gatcgccccg gtaattacgg cttttcagcg
    27901 gcgaaacggt cattggccag acccagttgc acagggtgac ggcgttatgc aaacagaccc
    27961 gcaacagcga ccacttagca tatttaattt ttgcgtcatt gattggcgtg tagtttttgg
    28021 tttcatcgta agcatcttca gaaccaaaaa gttctttcgc cacttcacgt tgcagtttca
    28081 atggcaagcc ggaaccaata aagttgatat gggtatgcgt catgcagtca cggttgaaca
    28141 tacagttaac aatggaaccg acctgcgctg acgcttcatt ggcatggtga accggatagc
    28201 caaatggcga ccagagttta tttttcgcgt agccccagta ctcttcaccc aaattccagc
    28261 gttctgcgat agcatatgaa ccatcagcca ggtgactcag ctcacccaca cgatgcgcca
    28321 gacggtagta aaaatcttta atgaagttaa cgtcacccgc ttccagttga tcccagcgaa
    28381 tttccgcata ctcttcagct ggtagaacac gcttgaacac ccctttgctg taacaataag
    28441 taaagtcgcg atgcagctgc ccatagttac accataggcc gtagtcatcg aatagattca
    28501 gaccaaccag gttaccaatc acacgaccat cgtctttatc ttcaaaatct ttcggcccgt
    28561 ttgggaagat ggtggtatgg acaaagtttg ctacacaagt gttaccaccg gttccaggca
    28621 cgccaaactc tttcacccga ggaatattca tttgggtcat acaacggatc gggcaagagt
    28681 ggcagccgct catttttact gtgtatttct ctgccgccgg acctaagtca aaaacggatt
    28741 tataggtacg aaagccgacc gtgttctgat tgcctggcgg aatctcaccc gtttcaatcg
    28801 gaccaccttc agccgcgccc caaaacagcc ctttacgtgc tgtccaacgt gacttgggat
    28861 ctgaatactc tgcccacgat tgcggcgtac ttggcacgac atggttgtta ttcgcaccga
    28921 taagttcagt catcatgtaa tcgttgagac gcttcatctc ctgacgatcg gcaatgttga
    28981 cccctttcgt cccttcaacc gcaatcgctt tcaggttttt cgaacccatt attgcgccag
    29041 ttcccgcacc accgctgtgg ttacggctat tcaacatgcc agagagagga acaaggtttt
    29101 ccccagcctg accaatagcc gccacacagg tttccggact ggtcaagcga caaatttctt
    29161 ccgtcgttgc gcgcgtccct tttccccata agaaatccgc tttttccagg ctaactttgt
    29221 catctttaat attcagccat accggtgatt tcgctttccc ttcgataata atgacgtcgt
    29281 agccagcgaa tttcatttgc gctgcaaaaa agccccccat atgggcatcg acgactaaat
    29341 ttcctttggt aaaagtagaa agtgaggtga tatttacgcg agaactgcag ggggcaccag
    29401 atccagttaa tgggccggta gcaaagacta atttattcgc ttcatcgaaa ggtttcgtgc
    29461 ctggcggtac ttcgtcatac ataattttgt agccgaagcc cataccaccg acaaaacttt
    29521 taaacttact ggaatcttcg agggtaatat ttcctgtcgt gagattgact cttaatatat
    29581 tacctgtcca accgttagcc atgatttttt cctttgcaag attcacacag taatatcttt
    29641 ccactcgata atttttaacg cccctgttgg gcaggcgttt gcgcattcac cgcataacac
    29701 acatttcgag gattttttac tttcggtatt tacggtggcc atcatccacg ggcacgccgt
    29761 cgtacaggcg ctacagccaa tacaacgttt atgatcgacg gtaatacagc cttcttcctg
    29821 ttgccaggta atcgcaccaa tcgggcagac gttcatgcat tgcggctctt tgcattgacg
    29881 gcaggtgtcc gcggtatagt tgagatcgcc atacaggccg ccgccagagc caaccccgtt
    29941 gtcgccaaag aaataattgc gatggatttt gatacgggag aagaatgtcc ctactgagcc
    30001 atcgttgaag ttggtacaag agatttcaca gcggtggcaa cccgtgcatc gagcacgttg
    30061 ggtcaccagc acccctttgg gtgtatttat caaccctact gtgccgctat caatatcttc
    30121 ctgtttgcaa cccaaaagtg acagtaacgc aggggcaatt gttaaaccag cgaggccttt
    30181 tcctgatata cgcaagaatt ctaatcgcgt taagccaata tctaatagtg gacgatcaac
    30241 cgggttcatt tatttcgcat cctcttcgca aacacagtgc tgacaattca gcagactgat
    30301 attaagcatt caactaatac gctgctgact ctgtccccaa caccagatat tttccctaac
    30361 ctgatagtgt atgagaaaag atttaacgcc gataaaccaa tcctttatgg ttaggagaaa
    30421 aaataatgtg attattctgg gcccttaaca ttgatcgtta tcaattaaaa tcacaaagca
    30481 agaaattata agtgaactga tatttattat catttgaaat aaatttaact taaacgacaa
    30541 ttatttgtga cgttcattgc agaaaataaa aataacatta tcgctataaa ttaaaataca
    30601 taccgaaatt cgtataattt cagcagttgt tatttagaat gattaactgg accgcaactg
    30661 aagcaagaat atcaggatga agcttatcgc ctcatcctga atggtcattt taccagtgcg
    30721 ttaatgacgg tttccatcgc ctgcttttcc agctcactgc gctgaatgcg tttattggct
    30781 aatgctgtac gtaatacgcc tgcgataaca atatgctttg gttcctgccc cagatcgcgc
    30841 atttccagca caactttacc gacaactctg cacatttctc ggtacagttc atcctctttt
    30901 gtccgatttc ccattcttta tacctattta tcatgccaac tatcagcata tatcaatcta
    30961 accaattaga caaaaatcaa acaaaatcag acaaataacg cgataaatta ttttaactgt
    31021 agcaattgag cgatgatgta tttatacacc ggatgaactt tcacttatct tcacagtgac
    31081 aaccccggca ccagacgttg cgcaaacagc gaagtttttg cgtaaccttt tccctggaac
    31141 gttaaatctt tgataacaat ttattgtctt acaagttgta tattttttga aacgctgttt
    31201 ttgttttcct tttggattaa tttcagcgta taatgcgcgc caattgactc ttgaatggtt
    31261 tcagcacttt ggactgtaga actcaacgac tcaaaaacag gcactcacgt tgggctgaga
    31321 cacaagcaca cattcctctg cacgcttttt cgatgtcacc tatccttaga gcgaggcacc
    31381 accactttcg taataccgga ttcgctttcc ggcagtgcgc ccagaaagca agtttctccc
    31441 atccttctca acttaaagac taagactgtc atgaaaaaga ccaaaattgt ttgcaccatc
    31501 ggaccgaaaa ccgaatctga agagatgtta gctaaaatgc tggacgctgg catgaacgtt
    31561 atgcgtctga acttctctca tggtgactat gcagaacacg gtcagcgcat tcagaatctg
    31621 cgcaacgtga tgagcaaaac tggtaaaacc gccgctatcc tgcttgatac caaaggtccg
    31681 gaaatccgca ccatgaaact ggaaggcggt aacgacgttt ctctgaaagc gggtcagacc
    31741 tttactttca ccactgataa atctgttatc ggcaacagcg aaatggttgc ggtaacgtat
    31801 gaaggtttca ctactgacct gtctgttggc aacaccgtac tggttgacga tggtctcatc
    31861 ggtatggaag ttaccgccat tgaaggtaac aaagttatct gtaaagtgct gaacaacggc
    31921 gacctgggtg aaaacaaagg tgtgaacctg cctggcgttt ccattgccct gccagcactg
    31981 gctgaaaaag acaaacagga cctgatcttt ggttgcgaac aaggcgtaga ctttgttgct
    32041 gcttccttta ttcgtaagcg ttctgacgtt atcgaaatcc gtgagcacct gaaagcgcac
    32101 ggcggcgaaa acatccacat catctccaaa atcgaaaacc aggaaggcct caacaacttc
    32161 gacgaaatcc tcgaagcctc tgacggcatc atggttgcgc gtggcgacct gggtgtagaa
    32221 atcccggtag aagaagttat cttcgcccag aagatgatga tcgaaaaatg tatccgtgca
    32281 cgtaaagtcg ttatcactgc gacccagatg ctggattcca tgatcaaaaa cccacgcccg
    32341 actcgcgcag aagccggtga cgttgcaaac gccatcctcg acggtactga cgcagtgatg
    32401 ctgtctggtg aatccgcaaa aggtaaatac ccgctggaag cggtttctat catggcgacc
    32461 atctgcgaac gtaccgaccg cgtgatgaac agccgtctcg agttcaacaa tgacaaccgt
    32521 aaactgcgca ttaccgaagc ggtatgccgt ggtgccgttg aaactgctga aaaactggat
    32581 gctccgctga tcgtggttgc cacccagggc ggtaaatctg ctcgcgcagt acgtaaatac
    32641 ttcccggatg ccaccatcct ggcactgacc accaacgaaa aaacggctca tcagttggta
    32701 ctgagcaaag gcgttgtgcc gcagctggtt aaagagatca cttctactga tgatttctac
    32761 cgtctgggta aagaactggc tctgcagagc ggtctggcac acaaaggcga cgtcgtagtt
    32821 atggtttctg gtgcactggt accgagcggc actactaaca ccgcatctgt tcacgtcctg
    32881 taatattgct tttgtgaatt aatttgtata ttgaagcgcc
//
LOCUS       DAPOZO010000054        32836 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPOZO010000054 DAPOZO010000000
VERSION     DAPOZO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.054"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            169..1446
                     /locus_tag="ROI21_002717"
     CDS             169..1446
                     /locus_tag="ROI21_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5111619.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            1443..2447
                     /locus_tag="ROI21_002718"
     CDS             1443..2447
                     /locus_tag="ROI21_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDX5111620.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKVELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            2444..3409
                     /locus_tag="ROI21_002719"
     CDS             2444..3409
                     /locus_tag="ROI21_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDX5111621.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIIRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(3383..4129)
                     /locus_tag="ROI21_002720"
     CDS             complement(3383..4129)
                     /locus_tag="ROI21_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDX5111622.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSNVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(4181..4999)
                     /locus_tag="ROI21_002721"
     CDS             complement(4181..4999)
                     /locus_tag="ROI21_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDX5111623.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIHPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKWVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(5064..5864)
                     /gene="thiD"
                     /locus_tag="ROI21_002722"
     CDS             complement(5064..5864)
                     /gene="thiD"
                     /locus_tag="ROI21_002722"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDX5111624.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTQNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(5861..6649)
                     /gene="thiM"
                     /locus_tag="ROI21_002723"
     CDS             complement(5861..6649)
                     /gene="thiM"
                     /locus_tag="ROI21_002723"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDX5111625.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAIEQAKRSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVASSEGPGSFVPHFLDALWQLTPEVQA"
     regulatory      complement(6684..6780)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6872..7144)
                     /gene="rcnR"
                     /locus_tag="ROI21_002724"
     CDS             complement(6872..7144)
                     /gene="rcnR"
                     /locus_tag="ROI21_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDX5111626.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            7265..8089
                     /gene="rcnA"
                     /locus_tag="ROI21_002725"
     CDS             7265..8089
                     /gene="rcnA"
                     /locus_tag="ROI21_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDX5111627.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGTLHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            8308..8646
                     /gene="rcnB"
                     /locus_tag="ROI21_002726"
     CDS             8308..8646
                     /gene="rcnB"
                     /locus_tag="ROI21_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDX5111628.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(8728..9762)
                     /locus_tag="ROI21_002727"
     CDS             complement(8728..9762)
                     /locus_tag="ROI21_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDX5111629.1"
                     /translation="MKIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTNGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLALSYNPITTTCKPDDLLITLESTPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLWGSSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEVAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(9778..12258)
                     /locus_tag="ROI21_002728"
     CDS             complement(9778..12258)
                     /locus_tag="ROI21_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX5111630.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCISREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGGWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYLQAGASVSGGIVAWSEGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRTDGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAINKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(12274..12948)
                     /locus_tag="ROI21_002729"
     CDS             complement(12274..12948)
                     /locus_tag="ROI21_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDX5111631.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(13029..13571)
                     /locus_tag="ROI21_002730"
     CDS             complement(13029..13571)
                     /locus_tag="ROI21_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5111632.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            complement(13867..14148)
                     /locus_tag="ROI21_002731"
     CDS             complement(13867..14148)
                     /locus_tag="ROI21_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDX5111633.1"
                     /translation="MNKYWLSGIIFLAYGMASPAFSSQTATLTINGRISPPTCSMAMV
                     NGQPQQYCGQLTYDVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(14411..15520)
                     /gene="apbC"
                     /locus_tag="ROI21_002732"
     CDS             complement(14411..15520)
                     /gene="apbC"
                     /locus_tag="ROI21_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDX5111634.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            15652..17685
                     /gene="metG"
                     /locus_tag="ROI21_002733"
     CDS             15652..17685
                     /gene="metG"
                     /locus_tag="ROI21_002733"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDX5111635.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGNTT
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWHGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            17826..21623
                     /locus_tag="ROI21_002734"
     CDS             17826..21623
                     /locus_tag="ROI21_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDX5111636.1"
                     /translation="MRHFIFQDEKSHKFWAVEQQGNELHICWGKVGTKGQNQVKSFAD
                     AAAAAKAELKLIAEKTKKGYVENALANVHIPPITKATPEVETSPESKNKRPWVADDAV
                     IPVTGDINRFAFPHRSRPREINYLYKDGQIWKRIANNTRAYDPDNNYRSYPENWQLAF
                     AELQRRILNNQQTGSIQSDAALLWNLWNSYSTDELVDDLVIRCGLETTVEIALLALQL
                     KYKPVKADVTTIIPPDLKAESLPNWHQRLCHHLSLASEDEWQRCVDKVLAAIPSLSPA
                     RQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDDAALTTAEKYDFPPLYHD
                     FRAYLAMLLANNGVRGVSRILLEFTEEHSDNPTYLFERISETEDLVKWLWKTNHPDAI
                     QILILGVIGKKKHLEYLSKACQKHPAAALAAYATLLAIHEDAQWRKALVKLITATPDL
                     VTDVIPWVNAKAAGILSECRTQLVAEECEYATADMLPELFVAPPWVINKKTNVIPVFD
                     LPVLPIPAVTDITPGITELISHTDISRFSEIAQYQSSQQTLFTDLPLIEKESWETSFI
                     PFTPEQQILWQLGFNEWLHCEDDLHEKKYIPQSAVDALLRFDFPALKAEFAKYHNNAN
                     KSWNLSALCYLPGQQAISFLNQIIIEERYSGEKEILAVFGSTAIPAFMTCLQRDHQRL
                     WIFTLFIGASELALPMAQRLQKKMAYKDAINWLANNPRHAAAGLLPLALGKPCQNREY
                     ARQAFRLLVKLNQRETIEEIGRRYNQPDVLAALATLFDSDPLEEYPAKIAPLPGFYQF
                     TLWRRPRLKSNNLPLPDDAMRHLGTMLSFPRDITSYAGLATIKETFTHESLADFGWDL
                     YTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRAWPGESQHKRAVYGLDVLADI
                     GSDVALMLLNGIARKIKFKALQEHAREKINIVAENRGLTMAELEDRLAPDLGLDNSGS
                     LILDFGPRQFTVGFDETLKPVVHDANGKVLKDLPKPNQSDDQTLATDSVNLFKKLKKD
                     VHAIASQQIDRLEQDMCQRRRWTAEQFRLFLVEHPLVRHLTRRVLWGVYTEENTLIAC
                     FRVAEDSTYSDAQDELFTLPAGNIGIPHVLEISPESATAFGQIYADYEKLPPFRQLDR
                     GYYHLTDNERDTHELIRWQGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGALE
                     LETEHFSLIYGETGYSDLLPVESVKITSPGERYSTQPSLTFSALDAITASELINDIES
                     LFD"
     gene            21636..25430
                     /locus_tag="ROI21_002735"
     CDS             21636..25430
                     /locus_tag="ROI21_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDX5111637.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELNISWGKVGTNGQSQVKSFAD
                     AAAAEKAELKLIAEKTKKGYVEHAENATSKHTRTVTKSLELSDFSTIIQEQPSVTPEF
                     YVADKNTDVILPWLAKDIAVVFPLEVVPTTLSHRRFPGDPVTPVDKLTQLRRLACGVS
                     QRDNKTATFDFSACTLEWQNFIAQAIDLINSPKTTKLPSPVMAVLAALELHCTRYKER
                     ADVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAVPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     VASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISQPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPISYLTEEISNQLLAKYIWYSKHITVSHEESTA
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLISEFKVFHSPTGHYWHLGILTTL
                     PLEKAVRAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRQDARTWLLKYPEHAITGLLSAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNAMLALDPLDNHPTKIPTLPAFYQPSIWTRPVLKAN
                     AQSLPDSALQHLGEMLRFHQEEALYPGLLQVKDACTADSLAEFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAISSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPALGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLQACFRVAEDNSYST
                     ADDDLFTLQEGDISVGIPHVLEISPTDAVAFGQLFADYELLPPFRQLERNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPADLSAEQVLSKLWLWEGKAESYGWGSNSTQEAQFSVLDDITASELINDIEAL
                     FE"
     gene            25440..27608
                     /locus_tag="ROI21_002736"
                     /pseudo
     CDS             25440..27608
                     /locus_tag="ROI21_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27729..29132
                     /locus_tag="ROI21_002737"
     CDS             27729..29132
                     /locus_tag="ROI21_002737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111638.1"
                     /translation="MAAAHLHAMALAQLRGHKLPLRTDWLDAIAGSLIKEALNAPLPW
                     SYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAELT
                     LNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEQWKLTRPLSQHAALIE
                     AACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLIAQ
                     ENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEKEF
                     HAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLDRRSQAIHAPALDRGAALGALMR
                     LEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLSDD
                     DFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHHQQ
                     LEQQALASLQHWGVFHV"
     gene            29125..30261
                     /locus_tag="ROI21_002738"
     CDS             29125..30261
                     /locus_tag="ROI21_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDX5111639.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGCSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            30258..32258
                     /locus_tag="ROI21_002739"
     CDS             30258..32258
                     /locus_tag="ROI21_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDX5111640.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAEFTHLIWQMRSEHVTSSDDPFANDEG
                     DACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPMPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAEVISGEGEDNVLTLSL
                     PASACSPFAVERMAALLQQTEDPVSMVSGFVSFVEGQLTLEPQVMMTKTRAWALDAET
                     TPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTAV
                     GFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            32383..>32836
                     /locus_tag="ROI21_002740"
     CDS             32383..>32836
                     /locus_tag="ROI21_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDX5111641.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKE"
BASE COUNT         8380 a         8304 c         7919 g         8233 t
ORIGIN      
        1 ggcgagtttt tccatctttt ggatgttttt tcagcgtttt ctgttggctc gattcatcag
       61 aaaaaatgtt accgcggtca agttttcacc gcaaaggtat ttaaaaggta ttattaaatg
      121 gtattgtcat cgcgtacctt acattacctg tcatgaagga attaaaagat gaaaacaaca
      181 gcaaagctgt cgttcatgat gtttgttgaa tggtttatct ggggcgcgtg gtttgtgcca
      241 ttgtggttgt ggttaagtaa aagcggtttt agtgccggag aaattggctg gtcgtatgcc
      301 tgtaccgcca ttgcggcgat cctgtcgcca attctggttg gctccatcac tgaccgcttt
      361 ttctcggcgc agaaagtgct ggcggtattg atgttcgctg gtgcggcgct gatgtatttc
      421 gctgcgcaac agaccacttt tgccgggttc ttcccgttac tgctggccta ctcgctaacc
      481 tatatgccga ccattgcgct gactaacagt atcgcttttg ccaacgtgcc ggatgtggag
      541 cgtgatttcc cacgcattcg tgtgatgggc actatcggct ggattgcctc cggtctggca
      601 tgtggatttt tgccgcaaat gctgggttat gccgatatct ctccgactaa catcccgcta
      661 ctgattaccg ccggaagttc tgctctgctc ggtgtgtttg cgtttttcct gcccgacacg
      721 ccgccaaaaa gcaccggcaa aatggacatt aaagtcatgc tcggcctgga tgcgctgatc
      781 ctgctgcgcg ataaaaactt cctcgtcttt ttcttctgtt cattcctgtt tgcgatgcca
      841 ctggcgttct attacatctt tgccaacggt tatctgacag aagtcggcat gaaaaacgcc
      901 accggctgga tgacgctcgg ccagttctct gaaatcttct ttatgctggc attgccgttt
      961 tttaccaaac gctttggtat caaaaaggta ttattacttg gtctggtcac cgctgcgatc
     1021 cgctatggct tctttattta cggtagtgcg gatgaatatt tcacctacgc gttactgttc
     1081 ctcggcattt tgctgcatgg agtaagttac gatttttact acgttaccgc ttacatctat
     1141 gtcgataaaa aagcccccgt gcatatgcgt accgctgcgc aggggctgat cacgctctgc
     1201 tgccagggct tcggcagttt gctcggctat cgtcttggcg gtgtgatgat ggaaaagatg
     1261 ttcgcttatc aggaaccggt aaacggactg actttcaact ggtccgggat gtggactttc
     1321 ggcgcggtga tgattgccat tatcgccgtg ctgttcatga tttttttccg cgaatccgac
     1381 aacgaaatta cggctatcaa ggtcgatgat cgcgatattg cgttgacaca aggggaagtt
     1441 aaatgaaaac agaacgtatt ctcggtgctc tttatgggca ggcgttaggg gatgcgatgg
     1501 ggatgccctc cgagctttgg ccacgcagcc gcgttaaagc acactttggc tggattgacc
     1561 gttttctgcc tggaccaaag gagaataacg cggcctgtta ttttaaccgc gccgaattca
     1621 ccgacgatac ctcgatggcg ctgtgtctgg cggatgcgtt actggaacgt aaaggcaaga
     1681 tcgatccgga tctgattggg cgtaatattc tcgactgggc gctgcgtttc gacgccttta
     1741 acaaaaacgt actaggtccg acctcgaaga ttgctctcaa cgccattcgc gacggtaaac
     1801 ccgttgctga actggaaaac aacggcgtga ccaacggcgc agcgatgcgc gtctcgccat
     1861 taggttgttt gcttccggcg cgtgatgttg attcctttat tgatgatgtg gcgctggcgt
     1921 ccagcccgac ccataaatcc gatctggcgg ttgcaggtgc ggtagtcatc gcatgggcga
     1981 tttcccgtgc cattgacgga gaaagctggt cagcgattgt tgattcacta ccctcgattg
     2041 cgcgacatgc acagcaaaaa cgcatcacca ccttcagcgc ctcactggcg gcgcgcctgg
     2101 agattgcgct gaaaattgtg cgcaatgccg acggcactga atccgccagc gaacagcttt
     2161 accaggtcgt tggcgcaggt accagcacta ttgagtccgt tccgtgcgcc attgcgctgg
     2221 ttgaactggc acaaaccgac ccgaaccgtt gcgccgtcct ctgcgctaac cttggcggcg
     2281 atacagacac catcggtgct atggcgacgg caatctgcgg cgcgttgcat ggcattaacg
     2341 ctatcgatcc tgcgttaaag gtggagctgg atgcggtaaa tcagcttgat ttcaaccgct
     2401 atgccacagc gctggcgaaa tatcgtcaac aacgggaggc ggtatgagcg gcgctcgatt
     2461 acacacgctg ctgcctgaat taaccacgcg tcagtcggtg atggtcgtcg gcgcggcggt
     2521 cattgacgtg atcgccgacg cttatgccct cccctggcgt gggtgcgata tcgaactgaa
     2581 acagcagagc gttaacgttg gcggctgcgc actgaatatt gccgtggcgt taaagcgcct
     2641 cggcatcgaa gcgggtaacg ccttgccgct cggtcagggc gtgtgggcgg agattattcg
     2701 caaccggatg gcaaaagagg gcttaatcag cctgatcgat aacgccgaag gtgataacgg
     2761 ctggtgtctg gcgctggttg agccggatgg cgaacgcact tttatgtcat tcagcggtgt
     2821 ggaaaatcag tggaatcgcc agtggctggc gcgattaacc gttgcgcctg gcagcctgct
     2881 ctatttttcc ggttatcaac tggcctcgcc ctgcggcgaa ttattagtgg aatggctgga
     2941 aaagctgcaa gacgtgacgc cgtttatcga ttttggccca cgtattggcg atatcccgga
     3001 tgcattgctg gcgcggatca tggcctgtcg acctttagtg tcactcaatc gtcaggaggc
     3061 tgagattgcc gccgaacgtt ttgctttatc cgcagagata acaacacttg gcaagcaatg
     3121 gcaggagaaa tttgccgcgc cgttgatcat tcgtctcgat aaagagggcg catggtattt
     3181 cagcaacgac gcttctggct gcattccggc atttccaacg caagtagtag acaccattgg
     3241 ggcgggcgac agtcatgcag gtggcgtact tgccgggcta gcctctggtc tgccactggc
     3301 ggatgccgta ttactgggca atgcagtggc gtcgtgggtt gtcgggcatc ggggcggtga
     3361 ttgtgcgcca acgcgcgagg aactactcct cgcacacaaa aacgtataga tcgctgcgac
     3421 agtggctgat gctgtactca ataggccgtt gctgttggtc aagcgccact tgcttgatca
     3481 ccagcaccgg tattttgcta tctaactgaa tatgtgactg aaactcggca tccggcatcc
     3541 gggcgctaac gcgggaacgc gtacgttgtg ggtaaatgtg ctggctgcgg aagtagtcat
     3601 aaagagaaat tccgatggca tcaacatcat gaattaaatg cgcaggaacc cacgattcct
     3661 caattgatac cgcttcttca tcgacatagc gaatacgctt aagcaaaaag acattacttc
     3721 ctgcttcgac cgctagctgc tgcgcgactt cttcgggaca tttcacgaca cgcttgttaa
     3781 cccatagtgt atcgggcttt ttcccgcgca ataccacctg ctgagaaaaa cctcgcgctt
     3841 ctttcaacga gtattcgaag atgttgttga tctgcgtacc gtaaccacgc gaacgcgtca
     3901 ccacgccctc ttcttccagc gcctgcatcg ccttgcgcac cgtaatgcgc gaaacgccgg
     3961 ttaactgact taaatcacgc tcaccgggca aaatattgcc atgctccagc accccgctgc
     4021 gcacggcatt ttttaccgtt tcggcaaact taatataaag cggcgtgtta tccgccgcta
     4081 aaatacgttc gttcagttgg gcaatcagct gggtatgcgc ttgttccatt tgtctttttc
     4141 ctgccaggga gtctggtggg agtatatcac tgcttcttat ttaaggcgtt tctttaatcc
     4201 catcaacgaa tgcctgcaac tcctccaccg tgccattaaa tacattaaaa tccaccggac
     4261 cattgatgcc atctacctgt ccacggtcgg aatgctgcca gaagcgccaa gccattccgt
     4321 cattgtccgg acgacgttga tagtaatgag ccacccacca cggatattca ttgaaatagc
     4381 ccgccagatt cgtgtgataa aaaacggctc ctgagtaaat aatcggcttt ttccccgtac
     4441 ttttttcgac catttttagc cactgactta cccacttgcg taattctttt gccgataatt
     4501 ttccccgttc ttctacgtcc agcacggcag ggaaatcgcc ttgtgagaaa tccaccgttt
     4561 gcagaaataa tctcgcctga actgaagcgg ataccgacgg ggaaaaataa tgatacgccc
     4621 cgcgcagcag gccattttcg cggcttagtt gccagttacg cgaaaaatag gggtccacca
     4681 gcttttcgcc ttccgtcgcc ttgataaagg caaattgcaa gcgaataccg ttatcgcgca
     4741 tttttgccac ccgctgccag tcgattcgct cctgccagcg cgaaacatct attccgtgaa
     4801 tggtgtaact ggcaggaatg cgaatagcga aagatttaac cgggcgataa ccgtagaagt
     4861 ggatgtagtc tttaatctga attgctgtcg aatagaaaaa attcaccgtc tgacgcggat
     4921 gaatcgcaac gatggaaatt agcccaagag cgcataataa agcggtgagt ttttttcgac
     4981 tggtgattct tagctgcatt ggtttcatcc ctgaatgtca gtgccaaagg ctgacaataa
     5041 ccaaagcagc tatagtacgg tgctcaccac caggcgtgga agtggtgaac cggaccaata
     5101 ccgtgaccaa cttccagcgt gtcggcctgg gctaacgccg atgaaagcca gctttttgcc
     5161 tcctgtacgg tgtcagccca gtttgtatgg cgcgggcgta gtgcagccaa cgccgcagag
     5221 agcgtacaac cagtgccgtg ggtgttttgg gtcataatgc gcggtgcagt aaatcgttgt
     5281 tccccctcac gagtaaacag ccagtcaggg ctttgctcat catccagatg accacctttc
     5341 attagcactg cgccacagcc catcgccaac agcgatcgcc cttgttccag catttcctgt
     5401 tcggtgcgcg cgtgtggcgc gtcgagcaag gcagcagctt cgggcaagtt tggcgttatt
     5461 aatgaaacct gtggcaataa tcgactgcgc agcgtagcaa ccgccgaagg tgaaagcagc
     5521 gggtcgccgc tttttgccag cataacggtg tcgagtacca cgttttggat ctgataacgt
     5581 tgcaaccgtt ctgccaccgc ttcaacaata tcggtttccg ccaacatacc gattttagtg
     5641 gtgtcgattc gcacatcgct gaacaccgaa tcgagctggg cggcgacaaa atcaggctca
     5701 atgcgataca ccgactgtac gccacgggta ttttgcgcca ccagtgcagt aatcactgag
     5761 caaccataag cgccaagtgc cgagaaggtt ttaagatcgg cctgaatccc cgcaccacca
     5821 ctcggatcag taccggcaat cgtcagagcg ttaattcgtt tcatgcctgc acctccggcg
     5881 tcaattgcca gagcgcatca aggaaatgtg gaacaaaact gcctggcccc tcgctactgg
     5941 cgactgcgcg ttctccggct tgtttcatcc agtgacaggc agatgcgata ttttccagcg
     6001 tatcgcctgg tagcgcacaa caggcagcga caaccgccga taatgcacag ccagttccta
     6061 ccactttggt cattaacgga tcgccaccgt gaataccaat gatacgatgt ccatcggtaa
     6121 cataatccat ctcgccagtg accacgacga ttgcgccagt ttcccgtgcc agtgtttgtg
     6181 cagcgggtat cgcattagct gcggcgtcag tggtatccac tccccgtcca ccattagcaa
     6241 cgccagctaa tgccatgatt tccgaagcat taccacgtat ggcggttggc ttatgagaca
     6301 gaagttccag acaaaaacgg cggcgataat cgagtgcacc caccgctact ggatcaagcg
     6361 tccagggtgt ttgagagcgt tttgcctgtt caatggcggc acgcatcgcc tgtgcgcgtg
     6421 gctgcgtcag cgtgccaacg ttaatcagca acgcactggc gatagccgca aactgactgg
     6481 cctcttcggt ttcgataacc atcgctgggg atgcaccgag cgccagcaag gtattggcag
     6541 taaaggtttg caccacatcg ttggtcatgc agtgcacaag aggggaatgt tggtgaaaaa
     6601 gatgtaacgc gtgcgcagat tgcgcggaac tcagcaggtc gacttgcata gtttgctcct
     6661 gccataacgt gaagaagcaa tgacctggtg gtccgtgact tccctacgct ggcattatcc
     6721 agatcaggtg atacgggtat ttctcagcct tcacgcagaa gggcaccccg agtcgtttga
     6781 ttgcgatgat aaatcgcaga ggaggatggt aatgtccagc gcacgcgttg taaacgagta
     6841 aaaaccgcag ttttacaatc gcgttaataa attatttgat ataagaatcc agcaccttca
     6901 gaacgacatc cagatcttct tcacgtttta gctcatcccc ctggtgaacg atgtgttccg
     6961 tcagatgacc tttaatcact tcccgcatca gaccgtttac cgcgccacgg atagcagcaa
     7021 tctgttgtaa aactgcagcg cattcgtgcg gctcgtcgag cattttcttg agcgccacga
     7081 cctggccctg aatcttactg gcacgcgctt tcagtttctg tttatcacgg atggtatgag
     7141 acatggcaac acctggttaa taagaatatg aaaaatcata gcactattaa tctactgggg
     7201 ggtagtatca ggtactgggg gggagtagaa tcagattgcc gaattaatac taagaattat
     7261 tatcatgacc gaatttacaa ctcttcttca gcaaggaaac gcctggttct tcatccccag
     7321 cgccatctta cttggtacgc ttcatggcct ggaaccaggg cactcaaaaa cgatgatggc
     7381 ggcgtttatc atcgccatca aaggcaccat taaacaggcg gtgatgctcg gactggcagc
     7441 aaccatttcg cataccgcag tggtctggtt gattgccttt ggcgggatgg tgatcagcaa
     7501 gcgctttact gctcaatcag cagaaccgtg gctccagttg atttccgcag tgatcattat
     7561 tggcaccgcg ttctggatgt tctggcgtac ctggcgcggc gaacgaaact ggctggagaa
     7621 tatgcacgag catgatcatg agcatcatca ccacgatcac gaagatcacc acgaccatgg
     7681 acatcatcac catcacgaac atggcgagta tcaggatgcc catgcacgag cccatgccaa
     7741 tgacattaaa cgacgctttg atggtagaga ggtcaccaac tggcaaattt tgctatttgg
     7801 cttaaccggt ggccttatcc cctgcccggc agcaattacc gtgctgttga tatgcattca
     7861 gttgaaagcc ctgacactgg gcgcaacact ggtcgtcagt ttcagcattg gcctggcgtt
     7921 aacgcttgtc accgtaggcg ttggcgcagc aatcagcgtt cagcaggtcg caaaacgctg
     7981 gagcggattt aacactctcg ctaaacgcgc cccctatttt tccagtctgt tgattggctt
     8041 agttggtgtg tatatgggcg tacatggctt catgggcata atgcgataac tcaatccttt
     8101 aggcttgcat gttatcttca gcccaggatg attcttaaat cagctatttc cgctgactct
     8161 ctgctcgcta aggatgcttt tagggcatcc tttcatttac actttttacg aaatcatggg
     8221 atcactaaca aaatatcgct tgccagttat attgtatggc aggaaagata tgcgactgat
     8281 attacagatc cccaaagtgg agagtttatg accattaaaa ataagatgtt gctgggtgcg
     8341 cttttgctgg ttaccagtgc cgcctgggcc gcaccagcca ccgcgggttc gaccaatacc
     8401 tcgggaattt ctaagtatga gttaagcagt ttcattgctg actttaagca tttcaaacca
     8461 ggcgataccg tcccagagat gtaccgcacc gatgagtaca acattaagca gtggcagtta
     8521 cgtaacctgc ccgcgcctga tgccgggacg cactggacct atatgggtgg cgcgtacgtg
     8581 ttgatcagcg acaccgacgg taaaatcatt aaagcctacg acggtgagat tttttatcat
     8641 cgctaaaaaa agccccctca tcatgagggg gaaatgcaga caccttgtta ttttttatta
     8701 ttagccactt gctcgtcttg cttgttatta gtcgtatttc acgttgatta atgcggttgc
     8761 ctccagtgcg ccagatttaa ctttgtttgt atcgtagacg tagtaactgg cagttatcgg
     8821 aattgaaacc gttgtatctg taagttttga cttcgcagca acttccttta aatttgtata
     8881 tcctttactg ctgttagtga tatttaaaag tgtgactggc gaaccattac tttcaagaat
     8941 aaaccctacg ccattatctt ccgcaccttt caggatcgtg ttactactgc cccacaaatc
     9001 agaacttgat aaatacacct gcatttttcg tgatgtttga ttttgttgac ctaataaatt
     9061 tttacaacgc agaataatat ctcctttttt actatttatt gttgctttgt tacctgtggc
     9121 tggtaattgt gaaacgggag tactctctaa agtaattaat aaatcgtccg gtttgcaggt
     9181 cgttgttatc gggttatatg acaatgccag attaaaggaa tagacacctg cattttgtgc
     9241 caatgcattt ctgtagctat ttacacatgc atcccatgac ttgccaccta ataaacacag
     9301 tagaaattgt actgcggcac tcccgcgtgt cgatgatgaa gcactggata atgtcgcaac
     9361 tccattaatc atcactgaac cgccgccccc cgcagaaaca ttcacttttg cattactggc
     9421 tgcaggtgtc accgttaccg aataaccatt ggtgaaactg gtctgattat ttgacaacgt
     9481 gatgttattt ttattggtcc attcaaaatg taaatttaat ttaatggtat cgttataaag
     9541 tccaacaata atatccgacg ggctcatagg ggtgatagta tttgtactca cgcatgacga
     9601 atcattaaaa ttatgctcat aggtcgccat ttgtgtttga gtaagtgcaa aatccccctg
     9661 aacaaccaaa ttgtgctgaa cagataactc actataacat gctgcatagc tcactggcat
     9721 aaccatcatc attaatataa atagcattat gcgaattttc atagaaccac ctgactatta
     9781 ctggcaaata taatttctac tttcatcaat ctctttaccg aaggtgattg tgcatgaaag
     9841 tccttgttgc ttattaattg ccacattaac cgacggcggt acttcattgg tgcgaataaa
     9901 taactggctt ccctggccga caacgccaat attatgacca tggatatcat tgacttcata
     9961 accaaacgtt aatggttgcc catccgttct taacgctttt ataaaccagg gcttgcgctg
    10021 atcggtatca aaattaacca gtacaaccgc gccgcgataa ggtgcggcaa ttttccggtt
    10081 accgcgcaat tctgcttcgc tatcgctttg cgacacatcc agcatcaggt gattttcccg
    10141 ataaggtgtc attccgtcgt ataccaccac tccattacgg tttgttgtgc gatatttttg
    10201 cccattgaca taagcatctt taattcctgg cgcattcatc actgcaaacg tttcggaaag
    10261 acggttcgcc agattaacgc cctccgacca ggcgacaatg ccccctgaaa cactggctcc
    10321 agcctgtaga taagtactcg actgactata actgccattc actgtcgcaa ccggcgcgtt
    10381 ccaggttaaa ttcgctccag ctgtcgtttc atttccctga tgctgatgac tcaggttgac
    10441 cccatagtta aattgatccc ggcttccaac ggttcccgat aatcccgtat tatttgaggc
    10501 aaaaccctga tcatcaaacg tcgttgagtt agacatatat atttgccgac gaggcgtcgt
    10561 aacgtcatca ccccaatcaa agggaatcga tataaaaata ttaaaacgtt tctcttcatg
    10621 atgattctcg tcataagcct ggcttgccgc gagggtatag cttatccgtc gcaggttgtt
    10681 ggaataactc aactgataat ccttactgct gccgctacgc ccccagtaat ctcgccataa
    10741 cgtacttaag gacacagaac cccagccttc tggcaatgac tggctcatat tggcagaaaa
    10801 gctattttta cggccaaaat cgttctggta ataatcggca atgtcataga tatcgttttc
    10861 atcacggcga taattatctt tattgtttgc ccaaacatga tcgttaaatg tccggtaatc
    10921 acgcgacgaa taacgccagg ccgccagacc aaaacgcgtc gacgtttggc tcacaaattt
    10981 gttgtaggca atttgataac tttgcccgtc aaacacatcg ccgttgtctt gtttactatg
    11041 cgacttcgtg gcatcgacgg aaatggcacc aatgcgtgta ttccagccag tccccaaagt
    11101 gaacgcgtaa taattattcg cgaccatcga tccgccatac agcgtcaata agttattaaa
    11161 accatactga taacccgcct ggacaaaatc actttgtttg ctcgcccctt caatatggct
    11221 acgacccgcc gcaaaatcat atttcgacac gccaggttgc agcatattcg gcaccgctgc
    11281 ataaggcacc agataggtgg ttaccgaacc gtccgcctct ttcacgctga catcaagatc
    11341 tgctccacca ccggccaact gcaaatctgt aatcgcaaac gggccaggag gaacctcttt
    11401 ctgataaacc acaaaaccgt tctgttcaat agttaccagc gcgttactct gagcaatccc
    11461 ctgcacccgt ggagtaaaat tctgtttcga gttaggcaac atctgcatat cacgaaacaa
    11521 ccgcacaccg ctgaagcgaa cagaatcaaa aatatcgctt gatgtgtaca tatcacccac
    11581 gcgaagcgtg ccgagaagtt gggcaaatcc acgttccaga tacagggtat tgcttttcca
    11641 cccccctgga ttgctatttg ttttactgaa actggcatca gaatgcagtt gccaccccag
    11701 taagtttaac ccgctgttaa aacgtacata tgtactcttg ctattacccg acgctttata
    11761 gtcgctgtaa tactgactca cataataaga ggtataaaac gcattaatac cccgctccca
    11821 gttttccggt ggaacatagc cactttccag ttcttccacc cacgcctgcg ggacactgaa
    11881 atcgagacga aaaactccga tatcccaggt atagctccca ccctgaacaa gttgctcaaa
    11941 tgttaaacat tgcttaccgc tggcaaagtt atcggtatta atgcctaacc gcttgataat
    12001 ttcccttgat atacatgttt cttgcgggtt gtctttaaca atgatctcat atttcccgcg
    12061 ccattgctta ttgacataaa tatcgatgtc atactgcccc ggcaagggtt gattatcatc
    12121 aagacgaata tttgcaacct gctggtcttt cattccacct atcataaaat gggtatcaaa
    12181 ggtttcttca gctgcataag cttcaatgcc gagcaataac gctacgattg ctgatgcaag
    12241 tggggtcatt ctcaacatag gcagctcctg caattaaatt ttgtcactaa tatagttgcc
    12301 atggtcatcg ataatggtca gataccagtt attcgcatta ttacttttga cattaatact
    12361 ctgactttct aagggggcaa tcataatagt ttcataattg actttgacat tattagcttt
    12421 gacatccgaa atcgttaccc aatttgctga gtcatttttt atcaccaatc cattgccact
    12481 gcgatttacc agtaactttt taaatgtcgc tttatttact ggcgcaatac ccgcaggccg
    12541 gtaaaacaat ttaattctgt tttgcattgc aaatttcagt gcattcttgc cctcttgctc
    12601 tggactattt ggtggaatat ccagaacatt cagataaaaa atgctctctt tattagtggg
    12661 cagtttattc ggcataattt tgatttttac ttgctgcccg gaatttgccc ctatttttgc
    12721 cactggtggc gttaacatga aaggaacctg aattttttct ggtggtaacg acgtatcgcc
    12781 atcatcaatc cacgcctgca gcagcgaaga acggtttccc tgattcatca actgcaccat
    12841 cacttcttta ttttctgccg ggtaaataat acgcgtcccg tagataacaa taccggcatg
    12901 tgcaggaagg accatagaag aaatgagtaa agctaataat cctttcatct taatgcctct
    12961 taaattaaaa ggccgccatg gggatagcgg ccatgtttat tacctggaga aacttgaagt
    13021 tttatttatt aatcctgcgc cacacgcata accagagaag ataacacatc acctgattgc
    13081 actgaattat ctgtcagctt caggtatttt gctttaaacg gaatagcata ttgaccttcc
    13141 gctgttttat aactctcaat gttaaaaggt gcagaaacat ccaggacatc gctgttatca
    13201 cctaaataga gatagtaccc cacggtatcg ttgctactgg ctatcgcttt atcgtttccg
    13261 gttattttat cgttggtaat caacgagagt tttacaccag caacttcttt cggacagcta
    13321 atttttaaca tgctgctggt tacaccgact gcatcatcat aggtcgagta tttagcttta
    13381 ccttcaaaga cagtggttgg tacagttggc atattaatac ttactgaatc gccacccgct
    13441 tcgaatttac acggagattc tgcaatattc cctttaatgg ttaatgttcc ggtatcaacg
    13501 tctgcggcaa ataccccagc gctcataaaa aaagaagaaa agacagcggc agcaataatt
    13561 gaacgtttca taatatgttc cttaataaaa tttatttttt ggttgttaag agtctgtatc
    13621 tcttggaaac aaatttctca catcaaaaaa gttgccgaaa tgtaaacata ttgttttatt
    13681 gatttaaaac aaattaacca ttgcaatata aacacaacaa atacttaagc atattaaata
    13741 tttatcacat ctattaatta atgcagattt ttgtaagtgg tattattgat ataaataaaa
    13801 aagctgaaat cattcacacg aatgatttca gctttctaaa tccgattaaa aaaccagggt
    13861 ggcaaattaa tcgtagcgat ttaacacgat tcgacgtttg ctatcgctac ctgcaacaac
    13921 cacttctgtc gttacacctt tgactggcga agaaacctga tgacgggtat caacatcata
    13981 cgttaactga ccacaatact gttgaggttg accattaacc atagccatac tgcatgttgg
    14041 gggactgatc ctgccattaa tcgttaatgt ggcagtctgt gaagagaacg cgggagaagc
    14101 cattccatag gcaagaaaaa taattcctga cagccagtac ttattcattg aacgttatcc
    14161 ctgtagtaaa ggttatgcct ggatagaatg agtgcataac aaactatagc tgtacatcca
    14221 ctacacagcc acgaaggatg ataatgaagc attgcctgta cgatcaatcg actttataga
    14281 gtttcggacg aaggtccgca gaatatttgc agcattaaat aagtgttcat taatgcggca
    14341 aaaacaaata caccgcatca atgtaacatc tctgcggtgt atgaaaaaca accaaaaagg
    14401 ttaatacgcg ttagaccgcg cggaaggaaa tctcgccagg aatgacttct ccctgccaat
    14461 aaagctgggc tgcaacgcgg tcagccagct ggcggtagat agcggtaaat tcgctctccg
    14521 gacggctgat aaccgttggc gtgcctttat cgagatcttc acgtaaggag atgtgtaacg
    14581 gcatctgccc cagcaactgc gtgtgatatt tctcggccag tttctctgca ccaccggtac
    14641 cgaaaatcgg ttcatggtga ccacagttac tgcaaatatg cacactcatg ttctcgacaa
    14701 taccgagcac cggaacctcg actttctcga acatcacaat gccttttttc gcatcgatca
    14761 acgcaatgtc ctgcggagtg gtgaccacta ccgcgccagt tactggtatg ttttgtgcca
    14821 gtgtcaattg aatgtcaccg gtgcctggcg gcatatcaag caccagatag tcgagatccg
    14881 gccacaaggt ttcctgcaac atctgcatta acgccttgct cgccatcgga ccacgccaca
    14941 ccatcgcatt gtcgtcagtg accagataac caattgaatt ggttgccagt ccatgagaca
    15001 taatcggtgc catatgggta ccgtccggcg aagttggacg ctgattttct gcacccagca
    15061 tggtgggtat tgatgggccg tagatatcgg cgtccagaat gccgactttc gccccttccg
    15121 ccgccagtgc cagcgccagg tttaccgccg tggaggattt acccacgccg cctttaccgg
    15181 agctgaccgc aatgatgttc ttcacgccgt taattcccgg ctgattcttc acgcgtttta
    15241 aggtggcgat attgtgcgac agtttccagt cgatagcttt cgccccggtg atgcgcagca
    15301 gttcggcgct acattgctct tttaactctt cgaacgcgct atgccagacg aatggcataa
    15361 ccagttccac atgcaacgtg tcgtccatcc aggcgacatg gtgcaacgct ttcagcgtgg
    15421 tgaggttatg cttcagggtt gggtgctgaa aattggccag tgtcccggcg accattgcgc
    15481 gcaaggcttc tggtgatttg gcctgggatt gttcgttcat cccgactcct tttttgtata
    15541 gataaaccat cagctgatag tttacctgaa gaatatagag aagtacttac ttaacatttt
    15601 tccatttggt actatctaac cccttttcac tattaagaag taatgcctac tatgactcaa
    15661 gtcgcgaaga aaattctggt gacgtgcgca ctgccgtacg ctaacggctc aatccacctc
    15721 ggccatatgc tggagcacat ccaggctgat gtctgggtcc gttaccagcg aatgcgcggc
    15781 cacgaggtca acttcatctg cgccgacgat gcccacggca caccgatcat gctgaaagct
    15841 cagcagctcg gtatcacccc ggagcagatg attggcgaaa tgagtcagga gcatcagact
    15901 gatttcgcag gctttaacat cagctatgac aactatcact cgacgcacag cgaagagaac
    15961 cgccagttgt cagaacttat ctactctcgc ctgaaagaaa acggttttat taaaaaccgc
    16021 accatctctc agctttacga tccggaaaaa ggcatgttcc tgccggaccg ttttgtaaaa
    16081 ggcacctgcc cgaaatgtaa atccccggat caatacggcg ataactgcga agtctgcggt
    16141 gcgacctaca gcccgactga actgatcgag ccgaaatcgg tggtttctgg cgctaccccg
    16201 gtaatgcgtg attctgaaca cttcttcttt gatctgccct ctttcagcga aatgttgcag
    16261 gcatggaccc gcagcggtgc gttgcaggag caggtggcaa acaaaatgca ggagtggttt
    16321 gaatccggcc tgcaacagtg ggatatctcc cgcgacgccc cttacttcgg ttttgaaatt
    16381 ccgaacgcgc cgggcaaata tttctacgtc tggctggacg caccgattgg ctacatgggt
    16441 tccttcaaga atctgtgcga caaacgcggc aacaccacca gcttcgatga atactggaag
    16501 aaagattcca ccgccgagct gtaccacttc atcggtaaag atatcgttta cttccacagc
    16561 ctgttctggc ctgccatgct ggaaggcagc aacttccgca agccgactaa cctgtttgtt
    16621 catggctatg tgacggtgaa cggcgcgaag atgtccaagt ctcgcggcac ctttattaaa
    16681 gccagtacct ggctgaatca ttttgatgct gacagcctgc gttactacta cactgcgaaa
    16741 ctctcttcgc gcattgatga tatcgatctc aacctggaag atttcgttca gcgtgtgaat
    16801 gccgatatcg tgaataaagt ggttaacctg gcatcccgta acgctggctt tatcaacaag
    16861 cgttttgacg gcgtgctggc aagcgaactg gctgacccgc agttgtacaa aaccttcacg
    16921 gatgccgctg aagtgattgg tgaagcgtgg gaaagccgcg aatttggtaa agcagtacgc
    16981 gaaattatgg cgctggctga tctggctaac cgctatgtcg atgaacaggc tccgtgggtg
    17041 gtggcgaaac aggaaggccg cgatgccgac ctgcaggcaa tttgctcgat gggcatcaac
    17101 ctgttccgcg tgctgatgac ttacctgaag ccggtactgc cgaaactgac cgagcgtgca
    17161 gaagcattcc tcaatacaga actgacctgg catggtatcc agcaaccgct gttgggccat
    17221 aaagtgaatc cgttcaaggc gctgtataac cgcatcgata tgaagcaggt tgaagcactg
    17281 gtggaagcat ctaaagaaga agtaaaagcc gctgccgcgc cggtaactgg cccgctggct
    17341 gacgatccaa tccaggaaac tatcaccttt gatgacttcg ccaaagttga cctgcgcgtg
    17401 gcactgattg aaaacgcaga gtttgttgaa ggttccgaca aactgctgcg cctgacgctg
    17461 gatctcggcg gtgaaaaacg caatgtcttc tccggcattc gttctgctta cccggatccg
    17521 caggcactga ttggtcgtca caccattatg gtggctaacc tggcaccacg taaaatgcgc
    17581 ttcggtatct ccgaaggcat ggtgatggct gccggtcctg gcgggaaaga tattttcctg
    17641 ctaagcccgg atgccggtgc taaaccgggt catcaggtga aataatctcc tttcaaggcg
    17701 ctgtatcgac agcgcctttt ctttataaat tcctaaagtt gttttcttgc gattttgtct
    17761 ctctctaacc cgcataaata ctggtagcat cagcattcaa ctggataaaa ttacagggat
    17821 gcagaatgag gcactttatc tttcaggacg aaaaatcaca taaattctgg gcggtggagc
    17881 aacagggaaa cgagttgcat atctgttggg gaaaagttgg caccaaaggg caaaaccagg
    17941 taaaaagttt tgcggatgct gcggcagcgg caaaagcgga gcttaagctg atcgcagaga
    18001 aaacgaaaaa aggctatgtg gaaaatgctt tagcaaacgt gcatatcccc cccatcacca
    18061 aagccactcc tgaagttgaa acctcccctg agagtaaaaa caaacgccca tgggtggctg
    18121 atgatgcagt cattccggta actggcgata ttaatcgatt tgcttttcct catcgctctc
    18181 gccccagaga gatcaattat ctgtacaaag atggccaaat atggaagcgc atcgcgaata
    18241 acaccagggc atatgacccc gacaataatt accgctctta tcctgaaaac tggcagctgg
    18301 cgtttgccga gttacaaagg cgaattctga ataatcaaca aacaggaagt atacaatctg
    18361 atgcggcatt gctctggaat ttatggaact cttactctac agatgaactg gtcgatgact
    18421 tagtcattcg ctgtggtctg gaaactaccg ttgaaatcgc ccttctcgct cttcaactca
    18481 aatataaacc agtaaaagcc gacgtcacca ccattattcc ccctgatctc aaagcagaat
    18541 cactaccgaa ctggcatcaa cgtctatgtc atcatctttc actcgcttca gaagatgagt
    18601 ggcaacgctg tgtagataaa gttctcgctg ctattccctc gctatcccca gcacggcaac
    18661 cttttgcggc gttactgctc cctgaacgcc cggatattgc caatgctatg gcgctacgtt
    18721 atgcagatca aaacgttccg gcgatgacct ggttaagact ggtggtaacc gatgacgccg
    18781 ctctgactac agcagaaaaa tacgacttcc caccgttgta tcatgatttt cgcgcttatc
    18841 tggcaatgtt gctggcaaat aatggggtgc gcggtgtaag ccggatactt cttgagttta
    18901 cggaagagca ttctgacaat cccacttatc tcttcgagcg catcagcgag actgaagatc
    18961 ttgtcaaatg gctatggaaa acgaatcacc cggatgcgat tcaaattctg atcctcggtg
    19021 taattggcaa gaaaaagcac ctggaatact taagcaaagc ctgtcaaaaa catcccgctg
    19081 cggctcttgc cgcttatgca actttactgg caatacatga agatgcacag tggcgtaaag
    19141 cgcttgtcaa actgattacc gccacgccag atttagtcac tgacgtcatt ccctgggtta
    19201 atgctaaagc ggcaggcatc ttatctgaat gtcgtactca gttagtcgct gaagaatgtg
    19261 aatacgccac tgcggatatg ttgccggagc tgtttgtcgc tccaccgtgg gtaataaata
    19321 agaaaacgaa tgtaataccc gtatttgatt taccggtgct tcccatccct gcagtcactg
    19381 acatcacgcc aggaattaca gagttaatca gtcatactga catatccaga ttcagtgaaa
    19441 tcgcgcaata tcagtcatca cagcaaacct tatttaccga cctgccacta attgaaaaag
    19501 aaagctggga gacatctttc attcctttta caccagaaca acaaatcctt tggcaattag
    19561 gttttaatga atggctgcat tgcgaagatg acttacatga aaaaaaatat ataccgcaaa
    19621 gcgctgttga tgccttgttg cgctttgatt ttccagcact aaaagctgag tttgcgaaat
    19681 atcacaacaa tgccaacaaa agctggaatc tatctgcgct ttgttactta ccgggacaac
    19741 aggcgatttc atttctcaac caaataatca ttgaggagcg atattcaggt gagaaagaga
    19801 ttctggcagt ttttggtagt acggccattc ctgcgtttat gacctgcttg caacgagatc
    19861 atcaacgact gtggatattc accctcttta ttggggccag tgaactagcc ttaccaatgg
    19921 cgcagcggtt acagaaaaaa atggcttaca aggatgccat taattggctg gcaaataacc
    19981 cgcgccatgc cgccgccggg ctactgcctt tggcgctggg caaaccgtgc caaaatcggg
    20041 aatatgcccg ccaggcattc cgcctgctag tgaaattaaa ccagcgagaa acgattgaag
    20101 aaatcggccg gagatacaac caacctgatg ttttagctgc gctggcaaca ttgttcgata
    20161 gcgatccgct ggaagaatat cccgctaaaa tcgccccact gcccggcttt tatcagttca
    20221 ccttatggcg cagaccacgg cttaaaagta acaacctgcc tctgccagat gacgctatgc
    20281 gccacctggg cacaatgttg agctttccac gcgacattac ctcctacgct gggctggcga
    20341 ccatcaaaga gaccttcacc cacgaatcac tggctgactt tggctgggat ctctacacgg
    20401 catggacaga agctggcgca cccgcaaaag aaaactgggc atttacttcg cttgggattt
    20461 taggcaatga cgacaccgca cgtaaattaa ccccgcttat ccgtgcctgg ccgggtgaat
    20521 cccagcataa acgcgcggtt tacgggctgg atgtattagc tgatattggc agcgatgttg
    20581 cgctaatgct gcttaatggc atcgcgcgaa aaattaaatt caaagcatta caggaacatg
    20641 cccgcgagaa aatcaacata gttgctgaaa accgtgggct gactatggct gaactggaag
    20701 accgcctggc tccagattta gggcttgata acagtggctc gctgatactg gatttcggcc
    20761 cacgtcaatt caccgttggt tttgacgaaa ccttaaaacc tgtagtgcac gatgcaaacg
    20821 gcaaagtcct gaaagattta cccaaaccaa atcagagcga tgatcaaaca ctggcaactg
    20881 actccgttaa cctcttcaaa aaattgaaga aagatgtgca cgccattgcc agccagcaga
    20941 ttgatcgtct ggagcaggat atgtgccagc gcagacgctg gacggcagag cagttccgcc
    21001 tgtttctggt ggagcatccg ctggtacgtc acttaacccg acgggtgtta tggggggtat
    21061 atactgagga aaacacgctg atcgcctgct tccgggtcgc agaagacagc acttacagcg
    21121 atgcgcagga tgagttattc acgctgccag caggaaacat cggtataccg catgtgctgg
    21181 aaatatctcc tgaatccgcc actgcatttg ggcaaattta tgctgattac gaaaagcttc
    21241 ctccctttcg ccaactcgat cgtggttatt accaccttac tgataatgaa cgtgataccc
    21301 atgaattgat acgctggcaa ggacgtcttt gccaggccgg gcgcattgtc gggctggaac
    21361 gtcgaggctg gcaacgtctg gaagaaagcg gcagcgtcta tgcgatgcgc aaatctaccc
    21421 cttatggcgc tctcgaactt gaaacagaac atttttcatt aatttatggc gaaacggggt
    21481 atagcgacct gctacctgtt gaaagcgtaa aaatcacttc accaggggaa cgctacagta
    21541 cacagccctc gctcacgttc tccgcgcttg atgccatcac cgccagcgaa ctcattaacg
    21601 acatcgaatc actttttgat taaaaggact ttgcaatgaa aacctatatt tatcaggacg
    21661 aaaaatcaca taaattctgg gcggtggagc agcagggaaa cgaactgaat atcagttggg
    21721 gcaaagtcgg caccaacggg caaagtcagg taaaaagctt tgcagatgct gcggcggcgg
    21781 aaaaagcgga actcaagctg attgcggaga aaacgaaaaa ggggtatgtg gaacatgcgg
    21841 agaatgctac atcgaaacat actcgaacgg taacgaaatc tctcgaatta tctgactttt
    21901 ctaccatcat tcaggaacag ccctcagtta caccagaatt ctacgtggct gacaaaaata
    21961 cagacgttat tttaccgtgg ctggcaaaag atattgctgt cgtttttccg cttgaagtcg
    22021 tacccaccac cttaagccat cgccgctttc ccggagatcc tgttacacct gtagataaat
    22081 taacccagtt acgtcgctta gcctgtggcg tgtcgcaacg ggataacaaa acagctacat
    22141 ttgacttcag cgcctgtact ttagagtggc aaaacttcat cgctcaggcg atcgatctga
    22201 ttaacagccc gaaaacgaca aaattaccat caccggtaat ggctgtactc gcggcacttg
    22261 aactgcactg cacgagatat aaagagcgtg cggatgttat ggatcagatt attcaggaag
    22321 gcggtctgga atatgctact gatgtaataa tacatcttca acaaatatct attgaatggg
    22381 attatgtcaa caataatatt gttttcctgt cgtctggcat ttcacctgat tatttgcagc
    22441 aatattccag ctttgaatta cgcctacgta aacatttatc actggcggaa gagtctctct
    22501 ggcaaaaatg tgcacaaaaa cttattgccg cagttccaca tattccagaa tggcggcaac
    22561 cattaattgc tttgttattg cccgaaaaac cagaaattgc acatgaaatt gcccagcgtc
    22621 tactggggca aaagaaatta ccctcgctgg agtggctaaa aatagtggcg actgatgagc
    22681 acattcttgc ctcattagaa aaatatcacg agccatatgc catttttgat gattactatt
    22741 gtggtgcgat atggtcagcc accgtattac aggagcaagg tgttgcggcc ctgccccgat
    22801 ttgctcccta tgtcgcaagt gactactgcg ccgatgtgtt gcgtcatatc aatcatccgt
    22861 tcgcattaac actgcttata cgtgtagcag ggcatactaa acgctgtcac gatcgaatga
    22921 cgaaagcctg cgctgcgttc ccacacgcag cactggcagc actggcggaa cttctagtgc
    22981 aaaaagaaga gaatagttgg cgcattatgc taatgacgat gcttatctcg caaccgacac
    23041 tagcagaaca ggtcattccc tggctttcga cacccgcagt tgcagtgctg aaatcatgcc
    23101 agcaacaact gacacagccc tcaaaccatg ccagcgccga tctactgccg gccatagtag
    23161 tctccccacc atggctttcg aaaaagaaaa aatcgccgat tccagtgctt gatttagcac
    23221 cattaggtat tgagccaatc agttatctga cagaagaaat cagtaatcaa cttttggcga
    23281 aatatatctg gtattcaaaa cacatcacgg ttagtcatga agaaagtact gccaatctgt
    23341 tggcaaggat gggttttcaa cgacggatcg ctggtacata tattaaagct cccgaagcgg
    23401 tagttgaggc atggctaaat gaagattatt caactttaat aagtgaattt aaggtgtttc
    23461 attcacctac cgggcattat tggcatttgg ggattttgac aacattgccg ctggagaaag
    23521 cagtaagagc atggaatgcc cttactctat ctccgcatac cgataccgaa tacgccatgt
    23581 tacattttgg actcaaagga ttacctgggt tagtaaactc acttgcacgc tatccacagg
    23641 aagccttgcc cataacgaat tacttcgcag cgagtgagct ggcacctgcc gtcgcccgcg
    23701 ccttcaacaa actcaaaacc ctacgacaag acgcccgcac ctggctgctg aaatacccgg
    23761 aacacgccat taccggcctg ctgtctgcgg cgctcggcaa agccggtgaa gcacaggata
    23821 acgcccgcgc tgcattgcgt atgcttaccg aaaacggtca tcaaccatta ctgcaagaaa
    23881 tcgcccggcg ttataaccag ccggaagtaa cagatgcggt gaacgctatg cttgcgctcg
    23941 atcccttaga taatcacccg acaaaaatcc ccactcttcc ggccttttat cagccatcga
    24001 tctggacccg cccggtatta aaagcaaatg cccaatcact gcccgatagc gcactccagc
    24061 acctcggtga aatgctccgc ttccatcagg aggaggcgct gtatccggga ttattgcagg
    24121 tgaaagatgc ctgtaccgcc gactcactgg ctgaatttgc ctgggatctg tttaccgcct
    24181 ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta ggcgttctcg
    24241 gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct ggtgaatccc
    24301 agcataaacg cgccaccgtc gggctggata tcctcgccgc tatcagtagt gatatcgccc
    24361 tgatgcagct taacggcatt gcccagaaac tgaaattcaa agcattacag gagcgggcga
    24421 aagagaaaat tgccgacatt gccgaaagtc gcgaactgac ggtagcggag cttgaagatc
    24481 ggttagcacc ggctctcggt ctggatgata acggttcgct gctgctggat tttggcccac
    24541 ggcagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgcgat gtttccggca
    24601 gccgcctgaa agacttacct aaacccaata aaagcgatga tgaatcacag gcaaacgatg
    24661 ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgccgcc cagcaggtag
    24721 caaggctgga atccgccatg tgcctgcgcc gccgctggtc gccggaaaac tttcagctct
    24781 tcctggttga gcatccgctg gttcgtcact taacccgccg tctgatttgg ggcgtttata
    24841 gcgccgaaaa ccagctacag gcgtgcttta gggtggcgga agataacagc tacagcaccg
    24901 ctgacgatga tcttttcacc ttgcaggaag gcgatatctc tgttggcatt cctcacgttc
    24961 tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac tacgaactgc
    25021 taccaccgtt ccgtcagctt gaacgtaaca gttacgccct gacagaagcc gagcgcaacg
    25081 ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc atggggctgg
    25141 caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatcgga tggatgatca
    25201 aacctctcgg tcgctggtcg ttaatcatgg aaatcgacga aggttttgcg gtgggtatgt
    25261 caccagccga tcttagcgct gagcaggtct taagcaagct gtggctatgg gaaggcaaag
    25321 cggaaagcta tggctggggg agtaattcaa ctcaggaagc gcagttctcc gtgctcgacg
    25381 acatcaccgc cagcgaactc attaacgata ttgaagccct gtttgaataa ggaaagagca
    25441 tggacaagga attaccgtgg ctggcggata acgcccaagt ggaactgaaa tataaaaaag
    25501 gcaaaacgcc gctcagccat cgcagttggc cgggcgaacc ggtgcctgtt atcactgaaa
    25561 gcctcatcca gacactgggt gatgaattgc tacaaaaagc tgagaagaaa aaaaacattg
    25621 tctggcgtta tgaaaatttt tcactggagt ggcagtcggc cattacgcag gccatcaact
    25681 tgattggcga acacaaaccc tcagttccgg cccggacaat ggcggcgcta gcctgtatcg
    25741 cgcaaaatga tagccaacag ttgctcgacg aaatcgtcca acaagaggga ctggaatatg
    25801 cgactgaggt ggtgattgca cgccagttta ttacacgatg ttatgaaagt gatcctctgc
    25861 tagtgacatt gcagtatcag aacgaggatt atggctatgg ttatcgatca gaaacctata
    25921 acgaattcga tctccgactg cgtaagcatc tctctctggc agaggaaagc agctggcagc
    25981 gttgcgccga caaactcatt gccgcactgc caggaataac caaagttcgc cgccctttta
    26041 ttgctctgat cctcccggaa aaaccagaaa tcgccaatga gttggtaagc cttgaatgcc
    26101 cgtggactca ttttcattct aaggagtggt taaaagttgt tgctattgac cccagagcgg
    26161 tgagaaaact cgaacgctac tggagccagg atatatttag cgatcgggaa gccagctaca
    26221 tgtcgcatga aaaccacttc ggctacgcgg cctgcgctgc ccttttgcgc gaacaaggac
    26281 tggcagccgt tccacgcctg gcaatgtatg cccataaaga agattgcggc agtctgctgg
    26341 tacaaattaa ccatccgcag gtcatccgca ccctgctgtt agttgctgat aaaaacaaac
    26401 ccagcctgca acgtgtagct aaatacagta aaaatttccc ccatgcgacg cttgccgcac
    26461 tggcagaact gctggcgcta aaagaaccac ctacgcgccc tggttatcca atcatcgaag
    26521 acaaaaagct gcctgcacag caaaaagcac gtgatgaata ctggcgtacg ctgttacaaa
    26581 cgctgatggc atcgcagcca caactggcag aagaggtgat gccgtggtta agtactcaag
    26641 cccggtcagt gctgaatagt tatttatcgg caccgcctaa aacggttatt gatagcaccg
    26701 ataacagcca aatgcctgaa atcctcgttt caccaccgtg gcgtagtaag aaaaaaatga
    26761 cagttccacg tcttgatttg gcaccgttcg aattaacgcc gcaagtttac tggcaaccag
    26821 gcgaacgaga gaggcttgcc gccactgagt ctgcccgtta tttcagcacg gaatctcttg
    26881 cggaacgcat ggaacataaa agtggacgag ttgtactaca ggagctgggt tttggagatg
    26941 atgtatggct gtttctgaat tatatactcc ccggaaaact ggatgctgca cgcaattcac
    27001 tcattgttca gtggcattac taccaggggc gggttgaaga gatcctgaat ggctggaact
    27061 ccccggaagc acaattagca gaacaggcgc ttcgcagcgg tcacgtagaa gtgttaatta
    27121 acatatggga aaatgacagc tactcacgct atcgtcggga aaagagtatc tggaacctgt
    27181 atttattggc acagttgccg cgtgagatgg ctttgacttt ctggctacgt atcaatgaga
    27241 aaaagcatct gttcgcgggt gaggactatt ttctcagtat cttcggattg gatgctctac
    27301 caggtctgct gttggctttt tcacatcgtc caaaagaaac atttccgtta attttaaatt
    27361 ttggcgcaac agaactggcc ctgcccgttg cccgcgtctg gcgacgtttc gcggcgcagc
    27421 gtgatctggc tcgccagtgg attttacaat ggccggaaca tacggctact gcacttatcc
    27481 ctcttgtctt caccaaatcc agcgataata gcgaagctgc attacttgcc ctacgtttac
    27541 tttacgaaca gggacatggt gaattgctgc aaaccgcggc aaaccgctgg cagcgtacag
    27601 atgtatggcc tgccccggtc tggcaaaact ggtgctgaca gtggggctta cagcaggcgg
    27661 gagaacaact gctaaaaacg gttctcaccc gtttgcgcca gcaccacttg cctgcttcga
    27721 ccgcggatat ggctgccgct catctgcatg cgatggcgct ggcacagttg cgcggacata
    27781 aactaccgtt acgcactgac tggctggatg ccatagcagg ctcgctgatt aaagaagccc
    27841 tgaacgcgcc gttgccgtgg agctatcgcg gcgttattca tcccgacacc gatccgattc
    27901 tgctaacgtt gatagacaca ttagcgggtg acggattcgg taaacttgcc ccttctacgc
    27961 cacaaccgcc tctgccaaaa gatgtcacct gcgaactgga acgtaccgca atctctcttc
    28021 cggcggagct taccttaaat cgctttaacc ccaatggact agcgcaaagt caggtgttac
    28081 atcggctggc aatactggag atcccaggga ttgtacgcca gcagggaagc acactgacac
    28141 ttgcaggcaa cggtgaagaa cagtggaaat taacccgccc gcttagccag catgcggcat
    28201 tgattgaggc cgcctgcttt ggtgccacac tccaggaagc cgcacgccat aaattagaag
    28261 ccgatatgct ggacgcgggt ggaatcggca gtatcaccac atgtcttagc caggcggcgt
    28321 tagcgggtct ggcgtccttc agtcaacaat tattggagca actcacatta ttaatcgccc
    28381 aggaaaatca atttgccgaa atgggccagg cgctggaagt gctatatgcc ttatggcggc
    28441 tggatgaaat tagcggtatg caaggcgcgc agatattaca aacgacgtta tgcgcggcta
    28501 tcgatcgcac gctgtggctg tgtgaatcta acggcagacc agatgaaaag gagtttcacg
    28561 ctcacctgca tagctggcag gcgctttgcc atatcctgcg cgatctacat agcggcgtta
    28621 atttacccgg cgtttctctt tctgcggcgg tagccttact ggaccgccgc agtcaagcga
    28681 ttcatgcccc ggcgctggat cgcggcgcgg ctcttggcgc actgatgcgt ctggaacatc
    28741 ccaacgccag tgccgaagcg gcgctgacaa tgctggcgca gttatccccg gcacaatccg
    28801 gcgaagcgct gcacggtttg ctggcgctgg cccgccatca actggcctgt cagccggcat
    28861 ttatcgccgg tttcagtagt catttaaatc aactgagtga cgacgatttt attaacgccc
    28921 tgcccgattt acgcgcggcg atggcctggc taccaccacg agaacgcggg acgctggcgc
    28981 atcaggtgct tgagcattat caactggcgc aacttcccgt ttcggcactg caaatgccgt
    29041 tgcattgtcc accacaagcc attgcacatc atcaacaact cgaacagcag gcactggcat
    29101 cgctgcaaca ctggggagtt ttccatgtct gaactgaacg atcttctgac cacccgtgag
    29161 ctacaacgct ggcgattaat tcttggcgaa gcggcagaaa cgacgctttg tgggctggat
    29221 gacaacgccc ggcagataga ccacgcgctg gagtggctgt atgggcgcga tcctgaacgg
    29281 ctccagcgtg gtgaacgctc cggtggatta ggttgctcaa atctcaccac ccctgagtgg
    29341 atcaacagta ttcacacgct gtttccgcaa caggtgattg aacggctgga aagcgatgcc
    29401 gtactgcgct acggcattga agatgtggtg acgaatctcg acgtgctgga acgtatgcag
    29461 ccttctgaaa gcctgctacg cgccgttttg cacaccaaac atctgatgaa tcccgaagta
    29521 ctggctgccg cccgccggat agtacgccag gttgttgaag aaattatggc gcgactggca
    29581 aaggaagttc gtcaggcttt ttctggtgtc cgcgatcgcc gtcgccgctc atttattcca
    29641 ctggcgcgaa actttgattt caaaagtacg ctgcgcgcca acctgcaaca ctggcacccg
    29701 caacacggca agttgtatat cgaatccccc cgctttaaca gccgcattaa acgccaaagc
    29761 gaacaatggc aactggtctt actggttgat caaagcggat caatggtgga ctcggtgatc
    29821 cactcagcgg tgatggcggc ctgtttatgg cagttacccg gtattcgtac ccatctggtg
    29881 gcgtttgaca ccagcgtggt tgatctcacg gcagatgttg ccgatccggt agagttatta
    29941 atgaaagtgc aattaggcgg cggaaccaat atcgccagtg ccatggaata tggtcggcaa
    30001 cttattgaac aaccagcgaa aagcgtcatt atcctcgtga gcgattttta cgaagggggt
    30061 tcatcatcat tactgacgca tcaggtgaaa aagtgtgtcc agagcggcat caaagtgctg
    30121 ggactggcgg cgctcgatag caccgcaaca ccttgctatg accacgatac ggcccaggcg
    30181 ctggttaatg tcggcgcaca aatagccgcc atgacaccgg gcgagctggc atcatggctt
    30241 gcggagaatc ttcagtcatg aattcactac gtccggaatt attagaactg acaccacaag
    30301 ctttgacggc gttaagcaat gccggttttg ttaagcgcag ccttaaagaa ctggaaaatg
    30361 gcaacgtccc ggaaatcagc catgagaacg gcgctttaat cgccaccttc agtgacggtg
    30421 tccgcaccca gctggcgaac ggtcaggcac tgaaagaggc tcagtgcagc tgcggggcca
    30481 gcggcatgtg ccgccatcgc gtgatgctgg ttttaagtta tcagcgactt tgcgccacca
    30541 cccagcctac ggaaaaagaa gaagagtggg atccggcaat ctggctggaa gaactggcta
    30601 ccctgcccga tgccacccgc aaacgcgcgc aggcactggt cgctaaaggt atcaccattg
    30661 agttgttctg tacgccgggc gaaattccct ctgcccgctt accgatgagc gatgtacgtt
    30721 tttattcccg cagcagcatt cgtttcgcac gctgtgattg tattgaagga acactttgcg
    30781 aacatgtcgt actggcagta caggcctttg tccaggccaa agcgcagcaa gcggaattta
    30841 ctcatttaat ctggcagatg cgcagcgaac acgtcacatc atctgacgat ccgtttgcca
    30901 acgatgaggg cgacgcgtgt cgtcaatatg ttcagcaatt aagccaggcg ttatggcttg
    30961 gcggcatcag ccagccgctt attcattacg aggctgcttt cagccgcgcg cagcaagcgg
    31021 cggaacgctg caactggcga tgggtgagtg aatcgctacg gcaactgcgc gccagcgttg
    31081 atgccttcca cgcccgcgcc agccactatc atgccggaga atgcttacgt cagcttgcgg
    31141 cattaaacag tcgattaaat tgcgcacaag agatggcgcg gcgcgacagt gttggtgaag
    31201 ttcctccgat gccgtggcgc acggtcgttg gcgctggcat tgccggagaa gcaaagcttg
    31261 atcatctgcg gctggtgtct ttaggtatgc gttgctggca ggatattgag cagtatggtt
    31321 tacgcatctg gttcaccgat cccgacaccg gcagtatttt gcacctttcg cgcagttggc
    31381 cgcgaagtga acaggaaaac tcaccggcag cgacgcgtcg gctatttagt tttcaggctg
    31441 gcgcactggc aggtgggcaa attgtttcac aagcagcaaa acgcagtgcc gatggcgagc
    31501 tgttgttagc tacccgcaac cgcttgagta gtgttgtgcc gctgtcgcca gacgcctggc
    31561 aaatgttgag cgcgccattg cgccagccgg gcattgtggc tttgcgggaa tatttacgcc
    31621 agcgtccccc cgcctgcata cggcctctta atcaggtgga taacttattt attctgccag
    31681 tcgctgagtg tatttcactc ggttgggata gcagccgcca gacgctggat gcggaggtaa
    31741 tcagcggcga aggtgaagat aacgtgctga cattatccct accagcctct gcctgttcgc
    31801 cctttgctgt tgaacgcatg gcagctctgt tacaacaaac agaggacccc gtgagtatgg
    31861 tttctggctt tgtcagtttt gtagaggggc aattgacact ggaaccacag gtaatgatga
    31921 caaagactcg tgcctgggct ctggacgcag aaactacgcc tgtggcaccg ctaccttctg
    31981 ccagcgtttt gcctgtgccg tctaccgctc atcagttgct gatgcgctgc caggcgttac
    32041 ttattcaact gctccataac ggctggcgct atcaggaaca gagtgctatt ggtcaggcag
    32101 agttgctggc gaatgacctc accgcggtgg gtttttatcg gctggcacat gtgttggcac
    32161 aatttcgtaa tacagaaagc gaggcacggg tagaagcaat gaataacggt gttttgcttt
    32221 gcgaacaatt attccccatg cttcagcaac aaggatgaaa tagtgttttt tactaagagt
    32281 tccactccag ttccggactg ctcacgccac ggtattaggc atatcctata tagcccctgg
    32341 tgagagtcac cagtttcttg attaaataaa atggagtttt acatgaaggc tttcaataag
    32401 ctgttttccc tcgttgttgc atctgttctg gttttctctc ttgctggctg cggtgacaaa
    32461 gaagaatcga aaaaattcag cgccaatctg aacggtactg aaattgccat tacctatgtc
    32521 tacaaaggtg acaaggtgct taagcaatct tctgaaacca aaattcaatt tgcctccatt
    32581 ggtgcaacca ccaaagaaga cgctgccaag acacttgagc cgttaagcgc caaatacaaa
    32641 aacatcgcgg gtgttgaaga aaaattaacc tataccgata cttacgcgca ggaaaacgtg
    32701 actatcgata tggaaaaagt ggatttcaaa gccctgcagg gtatttcagg aatcaacgtt
    32761 tctgctgaag atgccaaaaa aggtatcact atggcgcaaa tggaactggt gatgaaagcc
    32821 gctggtttta aagaag
//
LOCUS       DAPOZO010000055        32652 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPOZO010000055 DAPOZO010000000
VERSION     DAPOZO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.055"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            59..910
                     /gene="tsf"
                     /locus_tag="ROI21_002741"
     CDS             59..910
                     /gene="tsf"
                     /locus_tag="ROI21_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDX5111642.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            1057..1782
                     /gene="pyrH"
                     /locus_tag="ROI21_002742"
     CDS             1057..1782
                     /gene="pyrH"
                     /locus_tag="ROI21_002742"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDX5111643.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            2074..2631
                     /gene="frr"
                     /locus_tag="ROI21_002743"
     CDS             2074..2631
                     /gene="frr"
                     /locus_tag="ROI21_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDX5111644.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            2723..3919
                     /gene="ispC"
                     /locus_tag="ROI21_002744"
     CDS             2723..3919
                     /gene="ispC"
                     /locus_tag="ROI21_002744"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDX5111645.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTT
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            4108..4866
                     /gene="ispU"
                     /locus_tag="ROI21_002745"
                     /gene_synonym="uppS"
     CDS             4108..4866
                     /gene="ispU"
                     /locus_tag="ROI21_002745"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDX5111646.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANSGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            4879..5736
                     /gene="cdsA"
                     /locus_tag="ROI21_002746"
     CDS             4879..5736
                     /gene="cdsA"
                     /locus_tag="ROI21_002746"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDX5111647.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGYLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            5748..7100
                     /gene="rseP"
                     /locus_tag="ROI21_002747"
     CDS             5748..7100
                     /gene="rseP"
                     /locus_tag="ROI21_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDX5111648.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDECAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWTFEPDKEDPITSLGIRPR
                     GPQIESVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            7130..9562
                     /gene="bamA"
                     /locus_tag="ROI21_002748"
     CDS             7130..9562
                     /gene="bamA"
                     /locus_tag="ROI21_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDX5111649.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            9684..10169
                     /gene="skp"
                     /locus_tag="ROI21_002749"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             9684..10169
                     /gene="skp"
                     /locus_tag="ROI21_002749"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDX5111650.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            10173..11198
                     /gene="lpxD"
                     /locus_tag="ROI21_002750"
     CDS             10173..11198
                     /gene="lpxD"
                     /locus_tag="ROI21_002750"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDX5111651.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            11303..11758
                     /gene="fabZ"
                     /locus_tag="ROI21_002751"
     CDS             11303..11758
                     /gene="fabZ"
                     /locus_tag="ROI21_002751"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDX5111652.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            11762..12550
                     /gene="lpxA"
                     /locus_tag="ROI21_002752"
     CDS             11762..12550
                     /gene="lpxA"
                     /locus_tag="ROI21_002752"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDX5111653.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            12550..13698
                     /gene="lpxB"
                     /locus_tag="ROI21_002753"
     CDS             12550..13698
                     /gene="lpxB"
                     /locus_tag="ROI21_002753"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDX5111654.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHMLDGLGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            13695..14291
                     /gene="rnhB"
                     /locus_tag="ROI21_002754"
     CDS             13695..14291
                     /gene="rnhB"
                     /locus_tag="ROI21_002754"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDX5111655.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLVLCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            14328..17810
                     /gene="dnaE"
                     /locus_tag="ROI21_002755"
     CDS             14328..17810
                     /gene="dnaE"
                     /locus_tag="ROI21_002755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDX5111656.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAESRGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVMRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            17823..18782
                     /gene="accA"
                     /locus_tag="ROI21_002756"
     CDS             17823..18782
                     /gene="accA"
                     /locus_tag="ROI21_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDX5111657.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            18880..21021
                     /gene="ldcC"
                     /locus_tag="ROI21_002757"
     CDS             18880..21021
                     /gene="ldcC"
                     /locus_tag="ROI21_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDX5111658.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            21078..21467
                     /locus_tag="ROI21_002758"
     CDS             21078..21467
                     /locus_tag="ROI21_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDX5111659.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            21532..22830
                     /gene="tilS"
                     /locus_tag="ROI21_002759"
     CDS             21532..22830
                     /gene="tilS"
                     /locus_tag="ROI21_002759"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDX5111660.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELLA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(22879..23139)
                     /gene="rof"
                     /locus_tag="ROI21_002760"
     CDS             complement(22879..23139)
                     /gene="rof"
                     /locus_tag="ROI21_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDX5111661.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGTTRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(23126..23326)
                     /locus_tag="ROI21_002761"
     CDS             complement(23126..23326)
                     /locus_tag="ROI21_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDX5111662.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            23492..24037
                     /locus_tag="ROI21_002762"
     CDS             23492..24037
                     /locus_tag="ROI21_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDX5111663.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            24034..24456
                     /gene="arfB"
                     /locus_tag="ROI21_002763"
     CDS             24034..24456
                     /gene="arfB"
                     /locus_tag="ROI21_002763"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDX5111664.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRNQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            24470..25180
                     /gene="nlpE"
                     /locus_tag="ROI21_002764"
                     /gene_synonym="cutF"
     CDS             24470..25180
                     /gene="nlpE"
                     /locus_tag="ROI21_002764"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDX5111665.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSNLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPMLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(25210..26034)
                     /locus_tag="ROI21_002765"
     CDS             complement(25210..26034)
                     /locus_tag="ROI21_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDX5111666.1"
                     /translation="MDKPKAHCRLLLPCFFFLSACTVDISQPDSSATAVDAGAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(26087..27805)
                     /gene="proS"
                     /locus_tag="ROI21_002766"
     CDS             complement(26087..27805)
                     /gene="proS"
                     /locus_tag="ROI21_002766"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDX5111667.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQCGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(27916..28623)
                     /gene="tsaA"
                     /locus_tag="ROI21_002767"
     CDS             complement(27916..28623)
                     /gene="tsaA"
                     /locus_tag="ROI21_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDX5111668.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPNASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(28620..29024)
                     /gene="rcsF"
                     /locus_tag="ROI21_002768"
     CDS             complement(28620..29024)
                     /gene="rcsF"
                     /locus_tag="ROI21_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDX5111669.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RAAPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(29142..29957)
                     /gene="metQ"
                     /locus_tag="ROI21_002769"
     CDS             complement(29142..29957)
                     /gene="metQ"
                     /locus_tag="ROI21_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDX5111670.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(29997..30650)
                     /gene="metI"
                     /locus_tag="ROI21_002770"
     CDS             complement(29997..30650)
                     /gene="metI"
                     /locus_tag="ROI21_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDX5111671.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(30643..31674)
                     /gene="metN"
                     /locus_tag="ROI21_002771"
     CDS             complement(30643..31674)
                     /gene="metN"
                     /locus_tag="ROI21_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDX5111672.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            31862..32434
                     /gene="gmhB"
                     /locus_tag="ROI21_002772"
     CDS             31862..32434
                     /gene="gmhB"
                     /locus_tag="ROI21_002772"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDX5111673.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPV"
BASE COUNT         7878 a         7990 c         9014 g         7770 t
ORIGIN      
        1 tcacttttat atctgtgcgg tttaatgccg ggcagatcac atctccgagg attttagaat
       61 ggctgaaatt accgcatccc tggtaaaaga gctgcgtgag cgtactggcg caggcatgat
      121 ggattgcaaa aaagcactga ctgaagctaa cggcgacatc gagctggcaa tcgaaaacat
      181 gcgtaagtcc ggtgctatta aagcagcgaa aaaagcaggc aacgttgctg ctgacggcgt
      241 gatcaaaacc aaaatcgacg gcaactacgg catcattctg gaagttaact gccagactga
      301 cttcgttgca aaagacgctg gtttccaggc gttcgcagac aaagttctgg acgcagctgt
      361 tgctggcaaa atcactgacg ttgaagttct gaaagcacag ttcgaagaag aacgtgttgc
      421 gctggtagcg aaaattggtg aaaacatcaa cattcgccgc gttgctgcgc tggaaggcga
      481 cgttctgggt tcttatcagc acggtgcgcg tatcggcgtt ctggttgctg ctaaaggcgc
      541 tgacgaagag ctggttaaac acatcgctat gcacgttgct gcaagcaagc cggaattcat
      601 caaaccagaa gacgtatccg ctgaagtggt agagaaagaa taccaggtac agctggatat
      661 cgcgatgcag tctggtaagc cgaaagaaat cgcagagaaa atggttgaag gccgcatgaa
      721 gaaattcacc ggcgaagttt ctctgaccgg tcagccgttc gttatggaac caagcaaaac
      781 tgttggtcag ctgctgaaag agcataacgc tgaagtgact ggcttcatcc gcttcgaagt
      841 gggtgaaggc atcgagaaag ttgagactga ctttgcagca gaagttgctg cgatgtccaa
      901 gcagtcttaa tcatcgaaaa ggagccgcct gagggcggct tctttttgtg cccatcttgt
      961 aaattcagct aacccttgtg gggctgcgct gaaaagcgac gtacaatgtc gctagtatta
     1021 attcatttca atcgttgaca gtctcaggaa agaaacatgg ctaccaatgc aaaacccgtc
     1081 tataaacgca ttctgcttaa gttgagtggc gaagctctgc agggcactga aggcttcggt
     1141 attgatgcaa gcatactgga tcgcatggct caggaaatca aagaactggt tgaactgggt
     1201 attcaggttg gtgtggtgat tggtgggggt aacctgttcc gtggcgctgg tctggcgaaa
     1261 gcgggtatga accgcgttgt gggcgaccac atggggatgc tggcgaccgt aatgaacggc
     1321 ctggcaatgc gtgatgcact gcaccgcgcc tatgtgaacg ctcgtctgat gtccgctatt
     1381 ccattgaatg gcgtgtgcga cagctacagc tgggcagaag ctatcagcct gttgcgcaac
     1441 aaccgtgtgg tgatcctctc cgccggtaca ggtaacccgt tctttaccac cgactcagca
     1501 gcgtgcctgc gtggtatcga aattgaagcc gatgtggtgc tgaaagcaac caaagttgac
     1561 ggcgtgttta ccgctgatcc ggcgaaagat ccaaccgcaa ccatgtacga gcaactgact
     1621 tacagcgaag tgctggaaaa agagctgaaa gtcatggacc tggcggcctt cacgctggct
     1681 cgtgaccata aattaccgat tcgtgttttc aacatgaaca aaccgggtgc gctgcgccgt
     1741 gtggtaatgg gtgaaaaaga agggacttta atcacggaat aattcccgtg atggataaat
     1801 aagggtaaga ttccgcgtaa gtatcgcggg ggcgtaagtc tggttataag gcgttattgt
     1861 tacaggcagt ttggtcacgg ccagcgcgca gcaaccggag cgtacacaag tacgtgagga
     1921 tggcgagcac tgcccggggc caaaatggca aataaaatag cctaataatc cagacgatta
     1981 cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt gactgtctgg
     2041 tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag aaaagatgct
     2101 gaagtacgca tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg
     2161 ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg cacgccgacg
     2221 ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa aatcaacgtg
     2281 tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcgtccga tcttggcctg
     2341 aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac ggaagaacgt
     2401 cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt
     2461 aacgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa agagatcagc
     2521 gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc tgcaatcaag
     2581 aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg atttcttgaa
     2641 cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt gctttttatt
     2701 ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga ccggctcgat
     2761 tggttgcagc acgctggacg tggtgcgcca taatcccgaa cacttccgcg tagttgcgct
     2821 ggtggcaggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct ctccccgcta
     2881 tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa acgatgctac agcaacaggg
     2941 tagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggcag cgcttgagga
     3001 tgttgatcag gtgatggcag ccattgttgg cgctgctggg ctgttaccta cgcttgctgc
     3061 gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta cttgcggacg
     3121 tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg atagcgaaca
     3181 taacgccatt tttcagagtt taccgcaacc tatccagcat aatctgggat acgctgacct
     3241 tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt tccgtgagac
     3301 gccattgcgc gatttggcaa caatgacgcc cgatcaagcc tgccgtcatc cgaactggtc
     3361 gatgggacgt aaaatttctg tcgattcggc taccatgatg aacaaaggtc tggaatacat
     3421 tgaagcgcgt tggctgttta acgccagcgc cagccagatg gaagtgctga ttcacccgca
     3481 gtcagtgatt cactcaatgg tgcgctatca ggacggcagt gttctggcgc agctggggga
     3541 accggatatg cgtacgccaa ttgcccacac catggcatgg ccgaatcgcg tgaactctgg
     3601 cgtgaagccg ctcgattttt gcaaactaag tgcgttgaca tttgccgcac cggattatga
     3661 tcgttatcca tgcctgaaac tggcgatgga ggcgttcgaa caaggccagg cagcgacgac
     3721 aacattgaat gccgcaaacg aaatcaccgt tgctgctttt cttgcgcaac aaatccgctt
     3781 tacggatatc gccgcgttga atttatccgt actggaaaaa atggatatgc gcgaaccaca
     3841 atgtgtggat gatgtgttat ctgttgatgc gaacgcgcgt gaagtcgcca gaaaagaggt
     3901 gatgcgtctc gcaagctgag gataatccgg ctacagagag tcgcgctatt tgttagcgta
     3961 gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta taaggtcagg
     4021 tatgccgtgg ttttacacgg cttttttttg tataggcttc agtattcctg agtaccgtaa
     4081 accctgtcag ggaataagaa acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa
     4141 ttgccagcgc atggctgtcg tcatgttgcg atcattatgg acggcaatgg tcgctgggca
     4201 aaaaagcaag ggaagattcg tgcctttggg cataaagccg gggcaaaatc cgtccgccgg
     4261 gctgtctctt ttgcggccaa cagcggtatt gaggcgttaa cgctgtatgc ctttagtagt
     4321 gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg
     4381 ctcgatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat tattggcgat
     4441 accagtcgct ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc gctaacagcc
     4501 gggaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg ggatatagtc
     4561 cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc agatcagata
     4621 gatgaagaga tgctaaacca gcatgtctgt atgcatgaac tggcccctgt agatttagta
     4681 attaggactg ggggggagca tcgcattagt aactttttgc tttggcaaat tgcctatgcc
     4741 gaactttact ttacagatgt tctctggccc gatttcgatg aacaagactt tgaaggggcg
     4801 ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca
     4861 gcctgatggg ggtcgctttt gctgaagtat cgcctgatat ctgcttttgt gttaataccc
     4921 gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccattgt aacgctggtg
     4981 gtctgcatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac tcgctcgcag
     5041 cgagtatggt tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt tctgttgccg
     5101 gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc ttcgctgggt
     5161 tggtggattg tcgcgctatt gctggtgctg ttttacccag gttccgcagc aatctggcgt
     5221 aactctaaaa cattgcgcct tatttttggc gtgctaacca ttgttccctt cttctggggc
     5281 atgctggcgt tacgggcctg gcactacgac gagaatcatt acagtggcgc aatatggctg
     5341 ctctatgtca tgatcctggt atggggcgct gactctggcg catatatgtt tggcaaattg
     5401 tttggtaaac ataagctggc accgaaggtt tctccgggta aaacctggca aggctttata
     5461 ggtggactcg cgactgcagc ggtaatctca tggggttatg gcatgtgggc gaatctcgac
     5521 gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc agtgctcggc
     5581 gatctgaccg agagtatgtt taagcgtgaa gcaggaatta aggacagcgg ttatttaatt
     5641 ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt accggtcttt
     5701 gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg ctgagttttc
     5761 tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg catgaatttg
     5821 gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca atagggtttg
     5881 gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc gccctgatcc
     5941 cgctgggcgg ttatgtcaaa atgctggatg agtgcgcaga accggtcgtt ccggaactcc
     6001 gccaccatgc cttcaataat aaatctgtcg gccaacgagc ggcgattatt gccgcaggtc
     6061 cggttgcaaa cttcattttt gctatctttg cctactggct ggtttttatt attggtgtgc
     6121 ctggcgtacg tccggtggtt ggcgaaatag cagccaattc gatagctgca gaagcacaaa
     6181 ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat tgggatgccg
     6241 tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattaca gtagcgccat
     6301 ttggcagcga ccaacggcgg gatgtaaagc tcgatttacg tcactggacg tttgagcctg
     6361 ataaagaaga tccgataact tcgctgggga ttcgtcctcg tgggccgcaa attgaatctg
     6421 tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca
     6481 ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc
     6541 gggataaccc gggtaaatcc ttagcgttag aaatcgaaag gcaggggagt cccttgtctt
     6601 tgacattaat cccggagagt aaaccgggta atggtaaagc gattggtttt gtcggtattg
     6661 agccgaaagt cattcctttg ccagatgagt ataaagttgt acgccagtat gggccgttca
     6721 acgccatcgt cgaagccacg gacaaaacgt ggcagctgat gaagctgacg gtcagtatgc
     6781 tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg atctctatcg
     6841 ccaagggggc tgggatgaca gcggaactcg gggttgttta ttacctgccg tttcttgcgc
     6901 ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt gacggggggc
     6961 atctgctgtt ccttgcgatc gaaaagatca agggcggacc ggtatccgag cgggttcaag
     7021 acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt gcacttttca
     7081 atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga tggcgatgaa
     7141 aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg ctgaagggtt
     7201 cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct
     7261 cagtatgccg gtgcgcacag gcgacacggt taatgatgaa gatatcagta ataccattcg
     7321 cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg gtgataccct
     7381 tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg gtaacaaatc
     7441 ggtgaaagat gacatgctga aacaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc
     7501 cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct actacagcgt
     7561 cggtaaatat agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca accgtgttga
     7621 cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta acattgttgg
     7681 taaccatgct ttcaccaccg acgaactgat ctctcatttc caactgcgtg acgaagtgcc
     7741 gtggtggaac gtggtaggcg atcgtaaata ccagaaacag aaactggcgg gcgaccttga
     7801 aaccctgcgc agctactatc tggaccgcgg ttatgcccgt ttcaacatcg actctaccca
     7861 ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatca ccgaaggcga
     7921 tcagtacaag ctttctggcg ttgaagtgag cggcaacctt gccgggcact ccgctgaaat
     7981 tgagcagctg actaagatcg agccgggcga gctgtataac ggcaccaaag tgaccaaaat
     8041 ggaagatgac attaaaaagc ttcttggtcg ctatggttat gcctatccgc gcgtacagtc
     8101 gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg ttgatgcggg
     8161 taaccgtttc tacgtgcgta agatccgttt tgaaggtaac gatacctcga aagatgccgt
     8221 cctgcgtcgc gaaatgcgtc agatggaagg tgcatggctg gggagcgatc tggtcgatca
     8281 gggtaaggag cgtctgaatc gtctgggctt ctttgaaact gtcgataccg atacccaacg
     8341 tgttccgggt agcccggacc aggttgatgt agtctacaag gtgaaagaac gtaacaccgg
     8401 tagcttcaac ttcgggattg gttacggtac tgaaagtggc gtgagcttcc aggctggtgt
     8461 gcaacaggat aactggttag gtacgggtta tgctgttggt atcaacggga ccaaaaacga
     8521 ttaccagacc tatgctgaac tgtcggtaac caacccgtac ttcaccgtag atggcgtaag
     8581 cctcggtggt cgtctcttct ataatgactt ccaggcggat gacgccgacc tgtccgacta
     8641 taccaacaag agttatggta cagacgtgac gttgggcttc ccgatcaacg aatataactc
     8701 gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc ctcaggttgc
     8761 gatgtggcgt tatctgtact ctatgggtga acatccgagc acctctgatc aggataacag
     8821 cttcaaaacg gacgacttca cgttcaacta tggttggacc tataacaagc ttgaccgtgg
     8881 ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca ttccgggatc
     8941 ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga tcgatgacga
     9001 tcacaaatgg gttgttctgg gtcgtacccg ctggggttat ggtgatggtt taggcggcaa
     9061 agagatgccg ttctacgaga acttctatgc cggtggttct agcaccgtgc gtggcttcca
     9121 gtccaatacc attggtccga aagcagttta cttcccgcat caggctagta attatgatcc
     9181 ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gacctgtgta aatcggatga
     9241 tgctgtaggc ggtaacgcta tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt
     9301 cattagcgat aagtatgcta actcggttcg tacttccttc ttctgggata tgggtaccgt
     9361 ttgggataca aactgggatt ccagccaata ttctggatat ccggactata gtgatccaag
     9421 caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg ggccgttggt
     9481 gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac agttccagtt
     9541 taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat
     9601 gactttaggc gatcaatata agatcgccgg gccacgcaaa gaactgcacc ctccggtgca
     9661 aatgggatgg taaggagttt attgtgaaaa agtggttatt agctgcaggt ctcggtttag
     9721 cactggcaac ttctgctcag gcggctgaca aaattgcaat cgtcaatatg ggcagcctgt
     9781 tccagcaggt agcgcagaaa accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc
     9841 gtgccagcga actgcagcgt atggaaaccg atctgcaggc taaaatgaaa aagctgcagt
     9901 ccatgaaagc gggcagcgat cgcaccaaac tggaaaaaga cgtgatggct cagcgccaga
     9961 cttttgctca gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac
    10021 gcggcaaact ggttactcgt atccagactg ctgtgaaatc cgttgccaac agccaggata
    10081 tcgatctggt tgttgatgca aacgccgttg cttacaacag cagcgatgta aaagacatca
    10141 ctgccgacgt actgaaacag gttaaataag taatgccttc aattcgactg gctgatttag
    10201 cgcagcagtt ggatgcagaa ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt
    10261 ccatgcaatc tgcacaaaca ggtcacatca cgttcatggt taacccaaaa taccgtgagc
    10321 atttaggctt gtgccaggcg tccgcggttg tcatgaccca ggacgatctt cctttcgcga
    10381 aaagtgccgc actggtagtg aagaatccct acctgactta cgcgcgcatg gcgcaaattt
    10441 tagataccac gccgcagcct gcgcagaaca ttgcacccag tgcggtgatc gacgcgacgg
    10501 cgaagctggg taacaacgta tcgattggcg ctaacgcggt gattgagtcc ggcgttgaac
    10561 tgggcgataa cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg
    10621 caggttcgcg tctttgggcg aacgtaacca tttaccatga gatccagatc ggtcagaatt
    10681 gcctgatcca gtccggaaca gtggtaggcg cagacggctt tggttatgcc aacgatcgtg
    10741 gtaactgggt gaagatccca cagattggtc gcgtaattat tggcgatcgc gtggagatcg
    10801 gtgcctgcac aaccatcgat cgcggcgcgc tggatgacac tgttattggc aatggcgtga
    10861 tcattgataa ccagtgccag attgcacata acgtcgtgat tggcgacaat acggcggttg
    10921 ccggtggcgt cattatggcg ggcagcctga aaattggtcg ttactgcatg atcggcggag
    10981 ccagcgtaat caacgggcat atggaaatat gcgacaaagt gacggttacg ggcatgggta
    11041 tggtgatgcg tcccatcact gaaccaggcg tctattcctc aggcattccg ctgcaaccca
    11101 acaaagtctg gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg agcaagcgtc
    11161 tgaaatcgct tgagcgcaag gttaatcaac aagactaacg ttccatcttt tgttcgccaa
    11221 actttacggc ctgtctcatt cttacgattg cggcaggccg tgttattatt gtcgtttctt
    11281 atattttgac aggaagagta tcttgactac taacactcat actctgcaga ttgaagagat
    11341 tttagaactt ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc tggattttga
    11401 agaaggtcgt tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg
    11461 ccatttccct ggaaaaccga ttttcccggg agtgctgatt ctggaagcaa tggcacaggc
    11521 aacaggtatt ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc tgtactactt
    11581 cgctggtatt gacgaagcgc gcttcaagcg cccggtcgtg cctggcgatc aaatgatcat
    11641 ggaagtcact ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt
    11701 cgacggtaaa gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg aggcctgata
    11761 cgtgattgat aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcgat
    11821 tggcgcgaac gctcacattg gtcctttttg tatcgttgga ccccatgtcg aaattggtga
    11881 gggtaccgta ctgaaatctc acgttgtcgt gaatggtcat actaaaattg gccgcgataa
    11941 tgagatttat cagttcgcct ccatcggcga agttaaccag gatctgaaat atgctggcga
    12001 accgacccgc gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg
    12061 tggcacagtc cagggcggtg gattgacgaa ggtgggcagc gacaacttac tgatgatcaa
    12121 cgcgcatatt gcgcacgatt gtacggtagg taaccgctgt attctcgcca acaacgcaac
    12181 gctggcgggt cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga ccgcagtcca
    12241 tcagttctgc atcattggtg cgcatgtgat ggttggcggc tgctccggtg tggcgcagga
    12301 cgtccctcct tatgtcattg cgcagggtaa ccatgcaacg ccgttcggtg tcaatatcga
    12361 agggctgaag cgccgcggat tcagccgtga ggcgattacc gctatccgca atgcgtataa
    12421 gctgatttat cgtagcggta aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc
    12481 ggaaacatat ccggaagtga aagcctttac cgatttcttt gcacgctcaa cgcgcggtct
    12541 gattcgttaa tgactgaaca gcgtccatta acgattgccc tggtcgccgg agaaacctcc
    12601 ggcgatatcc tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc
    12661 tttgttggtg ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg
    12721 gaagaactgg cggtgatggg cattgttgaa gtgctcgggc gtctgcgtcg cttactgcat
    12781 attcgtgccg atctgacaaa gcgttttggc gaactgaagc cagatgtttt tgtcggtatt
    12841 gatgcgcctg acttcaatat tactcttgaa ggtaacctca aaaagcaggg tatcaaaacc
    12901 attcattacg tcagtccatc cgtctgggcg tggcgacaga aacgcgtttt caaaataggc
    12961 agagccaccg atctggtgct cgcatttctg cctttcgaaa aagcgtttta tgacaaatac
    13021 aacgtaccgt gccgctttat cggtcatacc atggctgatg ccatgccatt agatccagat
    13081 aaaaatggtg cccgtgatgt gctggggatc cctcacgatg cccactgtct ggcattgttg
    13141 ccgggcagcc gtggtgcgga agtcgaaatg cttagcgccg attttctgaa aacggcccag
    13201 cttttgcgcc agacatatcc ggatctcgaa atcgtggtgc cgctggtgaa tgccaaacgc
    13261 cgcgagcagt ttgaacgcat caaagctgaa gttgcgccag acctgtcggt tcatatgctg
    13321 gatgggttgg gccgtgaggc gatggtcgcc agcgatgcgg cactactggc gtcgggtacg
    13381 gctgcactgg agtgcatgct ggcgaaatgc ccgatggtgg tggggtatcg catgaagcct
    13441 ttcaccttct ggttggcgaa gcggctggtg aaaaccgatt atgtctcgct gccaaacctg
    13501 ctggcgggca gagagttagt taaagaatta ttgcaggaag agtgtgagcc gcaaaaactg
    13561 gctgcagcgc tgttaccgct gttggcgaac gggaaaacca gccacgcgat gcacgatacc
    13621 ttccgtgaac tgcatcagca gatccgctgc aatgccgatg agcaggcggc acaagccgtt
    13681 ctggagttag cacaatgatc gaatttgttt atccgcacac gcagctggtt gcgggtgtgg
    13741 atgaagtcgg acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg atccttgacc
    13801 cggcgcgccc gattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa cgccgtctgg
    13861 tgctctgcga agagatcaaa gagaaagcgt tgagctggag tctgggccgc gcggaacctc
    13921 acgaaatcga cgaactgaat attttacatg cgaccatgct ggcgatgcag cgtgccgtcg
    13981 ctggactgca tattgcgccg gaatatgtgt tgattgatgg taaccgctgc ccgaaattac
    14041 cgatgccttc gatggctgtg gtgaaaggcg atagccgcgt accggaaatc agtgccgcgt
    14101 ctatcctggc gaaagtgacg cgtgacgccg aaatggcggc gctggatatt gttttcccgc
    14161 aatatggttt tgcccaacac aaagggtatc caaccgcttt tcatctggaa aaactggctg
    14221 aacacggcgc gaccgaacac catcggcgca gctttgggcc tgtcaaacgc gcactgggac
    14281 ttgcgtcctg attcttgtgt cgagattaag taaaccggaa tctgaagatg tctgaaccac
    14341 gtttcgtaca cctgcgggtg cacagcgact actcgatgat cgatggcctg gccaaaaccg
    14401 caccgctggt aaaaaaggcg gcggcgttgg gtatgccagc actggcgatc accgatttca
    14461 ccaacctttg cggtctggtg aagttctacg gagcgggaca tggcgcaggg attaagccca
    14521 tcgtcggggc ggattttaac gtccagtgcg acctgctggg tgatgagtta acccacctga
    14581 cggtgctggc ggcgaacaat accggctatc agaatctgac gttgctgatc tcaaaagcgt
    14641 atcagcgcgg gtacggtgcc gccgggccga tcatcgatcg cgactggctt atcgaattaa
    14701 atgaagggtt gatccttctt tccggcgggc gcatgggcga cgttggacgc agtcttttgc
    14761 gtggtaacag cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac tttccggatc
    14821 gctattttct cgagctgatc cgcaccggca ggccggacga agaaagctat ctgcacgctg
    14881 cggtggaact ggcggaatca cgcggtttgc ccgttgtggc gaccaacgac gtgcgcttta
    14941 tcgacagcag cgactttgac gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc
    15001 tcgacgatcc taaacgcccg cgtaactatt cgccgcagca atatatgcgt agcgaagagg
    15061 agatgtgcga gctgtttgcc gacatcccag aagcccttgc caacaccgtt gagatcgcca
    15121 aacgctgtaa cgtaactgtg cgtcttggtg aatacttcct gccgcagttc ccgaccgggg
    15181 acatgagcac cgaagattat ctggtcatgc gtgcaaaaga gggcctggaa gagcgtctgg
    15241 catttttatt ccctgacgag gaagaacgtc tgaagcgtcg cccggaatat gacgaacgtc
    15301 tggagactga acttcaggtt atcaaccaga tgggcttccc gggctacttc ctcatcgtta
    15361 tggaatttat ccagtggtcg aaggataacg gcgtaccggt agggccaggc cgtggctccg
    15421 gtgcgggttc actggtggcc tacgcgctga aaatcaccga cctcgatccg ctggaatttg
    15481 acctgctgtt cgaacgtttc cttaacccgg aacgtgtctc catgcctgac tttgacgttg
    15541 acttctgtat ggagaaacgc gatcaggtta tcgaacacgt ggcagacatg tacggtcgtg
    15601 atgcggtatc gcagattatc accttcggta cgatggcggc gaaagcggtg atccgcgacg
    15661 taggccgcgt tctggggcat ccgtacggct ttgtcgatcg tatctcgaaa ctgatcccgc
    15721 ccgatccggg gatgacgctg gcaaaagcgt ttgaagccga gccgcagctg ccggaaatct
    15781 acgaagcgga tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg gaaggggtca
    15841 cccgtaacgc cggtaagcac gccggtgggg tggttatcgc gccgaccaaa attactgatt
    15901 ttgcgccgct ttattgcgat gaagagggca aacatccggt cacccagttt gataaaagcg
    15961 acgttgaata cgccgggctg gtgaagttcg acttcctcgg tttgcgtacg ctcaccatca
    16021 tcaactgggc gctggagatg atcaacaagc ggcgggcgaa gaatggcgag ccgcctctgg
    16081 atatcgccgc gatcccgctg gatgataaga aaagcttcga tatgctgcaa cgctcggaaa
    16141 ccacggcggt attccagctt gaatcgcgcg gcatgaaaga cctgatcaag cgtctgcaac
    16201 ctgactgctt cgaagatatg atcgcactgg tggcactgtt ccgccctggt ccgttgcagt
    16261 cagggatggt ggataacttt atcgaccgta aacatggtcg cgaagagatc tcctatccgg
    16321 acgtgcagtg gcagcatgaa agcctgaaac cggtactgga gccaacctac ggcatcatcc
    16381 tgtatcagga acaggtcatg cagattgccc aggtgctttc tggttatacc ctcggtggcg
    16441 cggatatgct gcgtcgtgcg atgggtaaga aaaagccgga agagatggct aagcagcgtt
    16501 ctgtatttgc tgaaggtgca gaaaagaacg gaatcaacgc tgaactggcg atgaaaatct
    16561 tcgacctggt ggaaaaattc gctggttacg gatttaacaa atcgcactct gcggcctatg
    16621 ctttggtgtc atatcaaacg ttatggctga aagcgcacta tccggcggag tttatggcgg
    16681 cggtaatgac cgccgatatg gacaacaccg agaaggtggt gggcctggtg gatgagtgct
    16741 ggcggatggg gctgaaaatc ctgccaccag atataaactc cggtctttac catttccacg
    16801 tcaacgacga cggcgaaatc gtgtatggta ttggcgcgat caaaggggtc ggtgaaggtc
    16861 cgattgaggc catcatcgaa gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc
    16921 tctgcgcccg aaccgacacc aaaaagttaa accggcgggt gctggaaaaa ctgatcatgt
    16981 ccggggcgtt tgaccgtctt gggccacacc gcgcggcgct gatgaactcg ctgggcgatg
    17041 cgttaaaagc ggcagatcaa cacgcgaaag cggaagctat cggtcaggcc gatatgttcg
    17101 gtgtgctggc cgaagagccg gaacaaattg aacaatccta cgccagctgc caaccgtggc
    17161 cggagcaggt ggtattagat ggggaacgtg aaacgttagg tctgtacctg acgggacacc
    17221 ctatcaatca gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca
    17281 tgcacccgac agaacgtggt aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg
    17341 ttatggtcac caagcgcggc aatcgtatcg gtatctgcac gctggatgac cgttccgggc
    17401 ggctggaagt gatgttgttt actgacgccc tggataaata ccagcaattg ctggaaaaag
    17461 accgcatact tatcgtcagc ggacaggtca gctttgatga cttcagcggt gggcttaaaa
    17521 tgaccgctcg cgaagtgatg gatattgacg aagcccggga aaaatatgct cgcgggcttg
    17581 ctatctcgct gacggacagg caaattgatg accagctttt aaaccgactc cgtcagtctc
    17641 tggaacccca ccgctctggg acaattccag tacatctcta ctatcagagg gcggatgcac
    17701 gcgcgcggtt gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg
    17761 atctccgtgg cctcattggt tcggagcagg tggaactgga gtttgactaa tacaggaata
    17821 ctatgagtct gaatttcctt gattttgaac agccgattgc agagctggaa gcgaaaatcg
    17881 attctctgac tgcggttagc cgtcaggatg agaaactgga tattaacatc gatgaagaag
    17941 tgcatcgtct gcgtgaaaaa agcgtagaac tgacacgtaa aatcttcgcc gatctcggtg
    18001 catggcagat tgcgcaactg gcacgccatc cacagcgtcc ttataccctg gattacgttc
    18061 gcctggcatt tgatgaattt gacgaactgg ctggcgaccg cgcgtatgca gacgataaag
    18121 ctatcgtcgg tggtatcgcc cgtctcgatg gtcgtccggt gatgatcatt ggtcatcaaa
    18181 aaggtcgtga aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt
    18241 accgcaaagc actgcgtctg atgcaaatgg ctgaacgctt taagatgcca atcatcacct
    18301 ttatcgacac cccgggggct tatccgggcg tgggcgcaga agagcgcggt cagtctgaag
    18361 ccattgcacg caacctgcgt gaaatgtctc gccttagcgt accggtagtt tgtacggtta
    18421 tcggtgaagg tggttctggc ggtgcgctgg cgattggcgt gggcgataaa gtgaatatgc
    18481 tgcaatacag cacctattcc gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga
    18541 gcgccgacaa agcgccgctg gcggctgaag cgatgggtat catcgctccg cgtctgaaag
    18601 agctgaaact gatcgactcc atcatcccgg aaccgctggg tggtgctcac cgtaacccgg
    18661 aagcgatggc ggcgtctctg aaagcgcagc tgctggcgga tctggccgat ctcgacgtgt
    18721 taagcactga agatttaaaa aatcgtcgtt atcagcgcct gatgagctac ggttacgcgt
    18781 aattcgcaaa tgttctgaaa agggtcactt cggtggccct tttttatcgc cacggtttga
    18841 gcaggctatg attaaggaag gattttccag gaggaacaca tgaacatcat tgccattatg
    18901 ggaccgcatg gcgtctttta taaagatgag cccatcaaag aactggagtc ggcgctggtg
    18961 gcgcaaggct ttcagattat ctggccacaa aacagcgttg atttgctgaa atttattgaa
    19021 cataaccctc gaatttgcgg cgtgattttt gactgggatg agtacagtct cgatttatgt
    19081 agcgatatca atcagcttaa tgaatatctc ccgctttatg ccttcatcaa cacccactcg
    19141 acgatggatg tcagcgtgca ggatatgcgg atggcgctct ggttttttga atatgcgctg
    19201 gggcaggcag aagatatcgc cattcgtatg cgtcagtaca ccgacgaata tcttgataac
    19261 attacaccgc cgttcacgaa agccttgttt acctacgtca aagagcggaa gtacaccttt
    19321 tgtacgccgg gtcatatggg cggcaccgca tatcaaaaaa gcccggttgg ctgtctgttt
    19381 tatgattttt tcggcgggaa tactcttaag gctgatgtct ctatttcggt taccgagctt
    19441 ggttcgttgc tcgaccacac cgggccacac ctggaagcgg aagagtacat cgcgcggact
    19501 tttggcgcgg aacagagtta tatcgttacc aacggaacat cgacgtctaa caaaattgtg
    19561 ggtatgtacg ccgcgccgtc cggcagtacg ctgttgatcg accgcaattg tcataaatcg
    19621 ctggcgcatc tgttgatgat gaacgatgta gtgccagtct ggctgaaacc gacgcgtaat
    19681 gcgttgggga ttcttggtgg gatcccgcgc cgtgaattta ctcgcgacag catcgaagag
    19741 aaagtcgctg ctaccacgca agctcaatgg ccggttcatg cggtgatcac caactccacc
    19801 tatgatggct tgctctacaa caccgactgg atcaaacaga cgctggatgt cccgtcgatt
    19861 cacttcgatt ctgcctgggt gccgtacacc cattttcatc cgatctacca gggtaaaagt
    19921 ggtatgagcg gcgagcgtgt tgcgggaaaa gtgatcttcg aaacgcagtc gacccacaaa
    19981 atgctggcgg cgttatcgca ggcttcgctg atccacatta aaggcgagta tgacgaagag
    20041 gcgtttaacg aagcctttat gatgcatacc accacctcgc ccagttatcc cattgttgct
    20101 tcggttgaga cggcggcggc gatgctgcgt ggtaatccgg gcaaacggct gattaaccgt
    20161 tcggtggagc gggcgctgca ttttcgcaaa gaggtccagc ggctgcggga agagtctgac
    20221 ggttggtttt tcgatatctg gcaaccgccg caggtggatg aagccgaatg ctggcccgtt
    20281 gcgcctggcg aacagtggca cggctttaac gatgcggatg ccgatcatat gtttctcgat
    20341 ccggttaaag tcactatttt gacaccgggg atggacgagc agggcaatat gagcgaggag
    20401 gggatcccgg cggcgctggt agcaaaattc ctcgacgaac gagggatcgt agtagagaaa
    20461 accggtcctt ataacctgct gtttctcttt agtattggaa tcgataaaac caaagcaatg
    20521 ggattattgc gtgggttgac ggaatttaag cgctcttacg atctcaacct gcggatcaaa
    20581 aatatgctac ccgatctcta tgcagaagat cccgatttct accgcaatat gcgcattcag
    20641 gatctggcgc aagggatcca taagctgatt cgtaaacacg atcttcccgg tttgatgttg
    20701 cgggcattcg ataccttgcc ggagatgatc atgacgccac atcaggcatg gcagaggcag
    20761 attaaaggcg aagtagaaac cattgcgctg gaacaactgg ttggtagggt gtcggcaaat
    20821 atgatcctgc cttatccacc gggcgtaccg ctgctgatgc caggagaaat gctgaccaaa
    20881 gagagccgca cagtactcga ttttctactg atgctttgtt ccgtagggca acattacccc
    20941 ggttttgaaa cggatattca cggcgcgaaa caggacgaag acggcgttta ccgcgtacga
    21001 gtcctaaaaa tggcgggata acttgccaga gcggcttccg ggcgagtaac gtgctgttaa
    21061 caaataaagg agacgttatg ctgggtttaa aacaggttca ccatattgcg attattgcga
    21121 cggattatgc ggtgagcaaa gcgttttact gcgatattct tggtttcacg ctgcaaagcg
    21181 aagtctatcg cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat gggcaatatg
    21241 tgattgagct tttctcattt ccgttcccgc cggagcgccc cagccgaccg gaagcttgcg
    21301 gcctgcgtca tctggctttt agcgttgatg atatcgatgc ggcagtggcg caccttgaaa
    21361 gccataacgt gaagtgtgaa gccatccgtg tcgatccata cacgcaaaaa cgcttcacgt
    21421 tctttaacga tccggacggg ctgccgttgg aactgtatga gcagtaaggc ttgtcatcgc
    21481 cgcatttgcc cggtaacgtg ccgggcattg ctactgtaaa atcgcaccat catgacactc
    21541 acgctcaata gacaacttct cacctcacgc cagattctgg tggcctttag cggcgggctt
    21601 gactccaccg ttttgctgca tcagttggtg cagtggcgga cggaaaatcc gggcgtcact
    21661 ctacgcgcta tccatgtgca tcacggttta agtgccaatg ccgatgcctg ggttaggcat
    21721 tgcgagaaca tctgccaaca gtggcaggtg ccgctggtgg tcgaacgcgt acaacttgcg
    21781 caagaggggc tggggattga ggcccaggcg cggcaggcgc gttatcaggc atttgcccgt
    21841 accctattgc ccggcgaagt gctggtcacc gcgcaacatc tcgacgatca atgtgaaacc
    21901 tttctgctgg cgctaaaacg cggtagcggc cctgccgggc tttcggctat ggcggaagtt
    21961 tcggagtttg ccggaacacg gcttatccgc ccgttgctcg cccgcacgcg gggggaactg
    22021 gagcagtggg cattggcgca tggtttacgc tggattgaag acgaaagtaa tcaggacgac
    22081 tcatacgatc gtaactttct gcgcctgcgc gtagtgccgt tattgcagca gcgttggccg
    22141 cattttgccg aagcaacggc ccgcagcgcc gcactttgtg ctgaacaaga gagcctgttg
    22201 gatgaactgc tggcggatga tttagcacac tgtcaatcgc cgcaggggac gctgcagatt
    22261 gcgccaatgc tggcgatgag tgatgcccgc cgcgcggcga ttatccgtcg ctggctggca
    22321 gggcagaatg caccgatgcc ttcccgcgac gcgctggtgc gcatctggca ggaagtggcg
    22381 ctggcgcggg aagatgcttc cccctgttta cgtctgggcg cgtttgaaat ccgccgctat
    22441 cagtcgcaac tgtggtggat taaatccgtt gccgggcaaa gcgaaaccat tgtgccgtgg
    22501 cagacgtggc tacaaccgct ggaattactg gcgggactgg gaagtgtaca gcttactgcg
    22561 ggaggcgata ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt caaagcgcca
    22621 ggattgctgc atatagtcgg acgcaacggc ggacgtaagc taaagaaaat ctggcaagag
    22681 ctgggtgtgc cgccgtggct acgtgacacc acgccactgc tgttttatgg cgaaacgttg
    22741 attgcggcgg caggggtatt tgtgacgcaa gaaggtgtgg ctgaaggtga gaatggcgtc
    22801 agttttgtct ggcagaaaac gcttagttaa gtgaaagccg gataagacgc ttcaaacgtc
    22861 gcatccggcg aaagtcaatc aggactcgct caccaccacc gtaccgattt ccgggtggct
    22921 aaagctggta attttatcca gacgcagctc gcgggttgta ccagcggcct cgacgaccag
    22981 atactccaca tttttgcggg agactaaatc gctggccttc gcctgcaatt tttcgccatc
    23041 tttcagctca agtgtcagca ttaaatgatg ctggcaggcg agctcaagat tatcgtaatc
    23101 atcacaattg attggttgat acgtatcatt cattgacata atcgctcacc agtaagtttg
    23161 ccgcagcgta tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg gcagccattt
    23221 cattcagcac tttcaaaacg cagcccagcg cgtccggaac gtatcctaag tctccgctgg
    23281 cgatttccgc gtaccgcttg cgtattaact cacaatattt ttccacatgc cctcctgtca
    23341 gcactctgac ttaaccgtgg atgcaagtct aagcctacga agataaactc tgtttcgcaa
    23401 ggtgactata ccacactcat ttctgcaata tcagcgccgc aactgcacgt attccgttac
    23461 aatggcctcc tgattcgaaa ggagttttct tatggcgctt aaagcgacaa tttataaagc
    23521 gacggttaat gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc tgacgctggc
    23581 gcgccaccct tcagaaaccc aggagcgcat gatgctgcgc ctgctggcgt ggctgaaata
    23641 tgctgatgaa cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc cggaagcgtg
    23701 gctgcgtaac gatcatttgg gcattgattt gtggatcgag ctggggctgc cggatgagcg
    23761 acggattaag aaagcctgca cccaggccgc agaagtagcg ctgtttgcct ataatagtcg
    23821 ggcggcgcaa atctggtggc agcaaaatca gagcaaatgt gcgcagtttg ccaatctttc
    23881 cgtctggtat ctggacgatg aacaactggc gaaagtaagc gcctttgccg atcgtaccat
    23941 gacgctgcag gcaacgattc aggatggcgt gatctggtta tcggatgata agaataatct
    24001 ggaagtgaac ttaaccgtct ggcaacaacc ttcatgattg tgatttcccg acatgttgct
    24061 atccccgatg gtgagcttga gatcaccgcc attcgtgcgc agggcgcggg cgggcagcat
    24121 gttaataaga cctcaacggc tattcatctg cgttttgaca ttcgggcgtc cagcctgcca
    24181 gagtattaca aagagcgtct gctcgccgcc agccatcatt tgatcagcag tgatggcgtg
    24241 attgtcatta aggcacagga ataccgcaat caggagctca accgcgaagc ggcgctggcc
    24301 cggctggtgg cagtgattaa agatttaaca acagaacaaa aagcccgacg acccacgcgg
    24361 cccacccgcg catcaaaaga gcgcaggctg gcatcgaaag cacaaaaatc aagcgtgaag
    24421 gcgatgcgcg gcaaagtgcg cagcggtcgg gaataaaaag aaggaatgga tggtgaaaaa
    24481 agcgatagtg acagcgatgg ctgtaatcag cctctttact ctgatgggat gtaataatcg
    24541 ggccgaagtc gacacgcttt ctccggcgca ggctgccgaa ctgaaaccga tgccgcaaag
    24601 ttggcgcggc gtgctgccgt gtgccgattg cgaagggatc gaaacctctt tgttcctcga
    24661 aaaagatgga acctgggtga tgaatgaacg ctatcttggt gcgcgtgaag agccttcctc
    24721 tttcgcttcc tacggtacat gggcgcgaac cgctgacaag ctggtattaa ccgacagcaa
    24781 aggtgaaaag tcatattatc gggcgaaagg cgatgcgctg gagatgctcg atcgtgaagg
    24841 taatccgatt gaatcgcagt tcaactatac gctggaaccg gcgcaatcca atttacccat
    24901 gacgccgatg accctgcggg gcatgtattt ttatatggct gatgcggcga ccttcactga
    24961 ttgcgcgacc ggaaaacgtt tcatggtagc gaataatgca gagctggagc gtggctacct
    25021 ggcggcgcgc ggtcatagtg aaaaaccgat gttactgtca gtagaaggtc actttacgct
    25081 tgaggctaat ccggataccg gtgcgccaac caaagtatta gcgcccgata cggcaggtaa
    25141 attttacccg aaccaggatt gcagtagttt ggggcagtaa ttgaaagccg gagcattctc
    25201 cggctttcat cactgagtca gcccaacaaa gcgacccgcc ttgatataaa tcccaggctt
    25261 caggtgccca acatactgta actgcgtttc tggcttaaac gcagagacat caccaatacg
    25321 catatgcatc agatcggcag ggctaatcca gcccgattgc gccagtgtaa gcggatgccc
    25381 ggctttggca aatgcatcag tgacaaattc cgaacaaaac cacgactttt tgtctccttc
    25441 gcccacactg cttaactgcg ctttcgccag gccgctgacg cactgttgac gaaaatcctc
    25501 ggagaacgga ttcagtgagc acatctggcg agtcaccata aagggaataa attcgacaat
    25561 gccgcgatag ttgtaaccgc tatctttaat tttgttggcg aatgcggtga tttctgtggc
    25621 ttgttgcggg gtaagatccg ggactcgtaa gacgaaaagc ttatcgctat gcttcatcgc
    25681 ttttttgagg gaaacaatct ggacgccagc gcctgtcgct tctgcaacgt tattttcacc
    25741 cagatagatt gcaacgtgac tcacagagga agtgctgaag acgcggattc caaatgaggt
    25801 taccccaagg cttgaggaga acagcaaatc gccgggtttg agatcaggtt ccgttatttc
    25861 ttttattgat tgttcggtaa aagagctttg atgctggaat ttgactgccc atgttttcgc
    25921 cccggcatct accgctgtgg ctgatgagtc cggctggctg atatctacgg tacaggccga
    25981 aaggaaaaaa aagcagggga gaagcaggcg gcaatgcgcc tttggtttat ccattttata
    26041 caatccatgt aaaaaagggc cctgaaattc aggacccttt ctggcatcag cctttaatct
    26101 gtttcaccag ataatcgacg atgtcaccag ttttaattaa ctgtttctcg ccgttacgac
    26161 gatatttata ttcgatatcg tcgttgtcga ggttacggtc gcccagcaca atagtgtgcg
    26221 gaataccgat cagttccata tcagcaaaca tcacgcccgg acgctctttg cggtcatcca
    26281 gcagcacttc gataccttgt gcacgcagtt cgctgtacag tttctcggcc agctcctgta
    26341 cgcggaagga tttatgcatg ttcatcggca gaatcgccac ctggaacggc gcgatagcgt
    26401 caggccatac gatgccgcgt tcgtcgtagt tctgctcaat tgccgcagct actacacgcg
    26461 ttaccccgat accgtagcaa cccatcgtca ggatttggtt acggccatct tcaccctgta
    26521 cggaggcttt cagtgcttca gagtacttag tacccagctg gaagatgtga ccgacttcga
    26581 taccacgttt gatcagcagc gtaccctggc catccgggct tggatcgcca gccaccacgt
    26641 tacggatatc agcaatttct ggggtagcga catcgcgatc ccagttgatg ccgaagtagt
    26701 gtttaccatc gatgttagca ccagcagcga aatcactcat cgctgcaacg gtacggtcaa
    26761 tcaccaccgg aatcggcatg tttaccggac ccagtgaacc cggaccggct ttaaccacgg
    26821 cacgaatttc ttcttcggtc gcaaaagtca gcgggcttgc aacctgcggc agtttttctg
    26881 ctttaacttc gttcagctcg tggtcaccac gcaccagcag cgcaaccagc gggaagctac
    26941 tgccttcaac cgctttaacc agcagagtct tcaccgtttt ctcaatcggc agattgaact
    27001 gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc aaccagcgtc atttcctggg
    27061 tagcagcagc gcgcggttct ttcggcgcga tagcttctgc cagttcaatg ttcgctgcat
    27121 agtcagaggt gtcggagaag accacatcgt cttcaccgca ctgcgccagc acctggaatt
    27181 cgtgagaggc gctaccgccg atagaaccgg tgtcggcctg tacggcgcgg aaatccagcc
    27241 ccatgcggct gaagattttg ctgtaggccg catacattgc atcgtaggtt tcctgcaggg
    27301 attcctgaga agtatggaaa gagtaagcat ctttcatcag gaattcgcgg gaacgcatga
    27361 cgccgaaacg cggacgcact tcgtcgcgga acttggtctg gatctgatag aagttcagcg
    27421 gcagctgttt gtaagagcta agctcgttac gaatcaggtc tgtaataact tcttcatgag
    27481 ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa acgcagcagt tccggaccgt
    27541 actgttccca acgaccactc tcttgccaca aatcggctgg ctgaaccacc ggcatcgaca
    27601 cctcgatcgc accggcgttg ttcatctctt cacgcacgat gttttcgact tttttcagaa
    27661 cgcgcacgcc ggtcggcagc caggtatata acccggaggc cagcttgcgg atcatcccgg
    27721 cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc aggtgtctcc ttgagagtgg
    27781 agagcaggta ttggctagta cgcatgttgt tacggttcca gttggaaggt agaacaggct
    27841 caaggcgagc ctgggacaaa aaaagtgatt tagtttacca gtgcaaaaga aatgtcaaaa
    27901 gagaagggcg cgaatttaac gcggttccag cgcaaagact tcaaaacctg cgtcggtgac
    27961 gcgccagcga acgttaaaat catgcagcca gacggcatag gttttgcccg tttcctcacc
    28021 tttacgatag gccgggcgcg ggtcctgcgc cagtacttcg cggataaaca gcgttaactg
    28081 cggataacgt ttctccagcg tcaaaagctg cttttcgacc tctgcggtaa agctcaccgc
    28141 catctctgcc gctggcgcac tttgcgcata gctggcactg gcatttggaa gcgattcggc
    28201 aaaggggaga taaggtttga tatccaccac cggcgtacca tctaccagat ccagactgcc
    28261 gagcttcaga atcacgctgt ctttatggca aacaacctct ttcagctcta ccagcgacat
    28321 accaatcggg ttagggcgga aagtggagcg cgtagcgaaa acacccattc tggcattacc
    28381 gccgaggcgc ggtggacgca cagtcggacg ccagccgcct tccatcgttt gatgaaagac
    28441 gaaaaggatc cataaatggc tgaacgcttc caggccgcga acggcgtcgg cctggttgta
    28501 gggagcaatg agatgcagtt cgccgttggc gctttttacc agacctggct ggcgcggaac
    28561 ggcgaatttt tctttatagg gcgagcgaat aacgcctatt tgctcgaact ggaaactgct
    28621 catttcgccg taatgttaag cgcagaaccg atacatacag cctgacgata gcagcctggc
    28681 gtaccgctgg tgacttcgca gctatgcagt aataccgcat tggccttcat tttcgaggcg
    28741 ttgatttgca tccgcttacg tgcggttgga atgctcggcg gagagtcctg attggaggcc
    28801 tggcaagaat cgccactgac ttcaccgaga tcgcggaacg gtttgccgac taattcttct
    28861 gcattggtat aaattcggac cggcgcggcg cgcggcgctt tcggttttgc aggctccgct
    28921 ttcggctggg gtgcagtgct ttgaacgggt tcgacagggg atctgcttaa catggaacag
    28981 ccgcttagca tgagtgctac taaacagatc ggtaaagcac gcatagtatt tcctcaatgt
    29041 atgatcaaaa cgtcaatatt gaatcaggag cttgtaaaaa tgacaagacg ggcaagcgcc
    29101 cgtcctgaat gatattacaa attgtggaaa cagcctaaaa attaccagcc tttaacagcg
    29161 ccgccgttaa acactttgtt tgctgcttcg taaacttcgt cagactgata agcctggacg
    29221 aatttcttca cgttctcggc gtctttgtta tcttcacgcg ttacgatcag gtttacgtac
    29281 ggggagtctt tatcttcgac aaagataccg tctttcgccg gagtcaggcc aatctggctg
    29341 gcataggtgg tattgataac tgccagagcg atttgcgcgt cgtccagaga gcgaggcagc
    29401 tgcggtgctt ccagctcaac aattttcaga ttttttgggt tctcaacaac atcaagaacg
    29461 gtcggcagca ggccaacacc atctttcagt ttgatcaagc ccactttttg cagcagcagc
    29521 agagaacggc caaggttagt tgggtcgttt ggcacggcaa cctgcgaacc atcctgcaat
    29581 tcatccagtg atttgatttt cttggagtaa ccagcaatcg gataaacaaa agtgttgcct
    29641 actgcgacca gtttgtagcc acgatctttc agttgctgat caaggtacgg tttatgctgg
    29701 aaggcgttgg cgtcgatatc gcctttgctc aatgcctcgt ttggcagaac atagtcgttg
    29761 aaggttacca gctcaacgtc cagaccatat ttgtctttcg caactttctg cgcgacttct
    29821 gcaacctgct gttcggcacc aacaatcacg ccgactttaa tgtggtttgg atctttttca
    29881 tcctgaccgc agcctaccaa tgccagtgat ccaatcaggg ctcccactgc cgcaaaggtt
    29941 ttgaatttga acgccatacc ttattccttc ttcaattatt tatgttgtgt tgaacgttac
    30001 ttgcgagtga cagcccggac gatgcggtcg cctgcgaact gaattaaata aaccagaatg
    30061 accagcaata ccagtaccgt attcatcacc gtcgcgttgt agccgatgta gccatactga
    30121 tagccaatct gacctaaacc accggcaccg actgcaccac ccatcgcgga ataaccgacc
    30181 agggtaatca gggtgatagt cgcagcattc accagacccg gcaatgcttc cggtaacagc
    30241 actttacgga cgatctgcat cggcgtggca cccattgcgc gggaagcttc aattagcccg
    30301 gttgggatct ccagcagagc gttctcgacc atacgggcaa taaacggtgc tgcaccaacg
    30361 gttaacggaa caatcgctgc ctgcaaacca atcgatgtac caacaataac gcgggtaaac
    30421 ggaatcatcc ataccagcaa gataatgaac gggatggaac ggaaaatgtt cacaatcgca
    30481 gaaacggtac gatacagctt cgcgttagca ataatttgcc ccggacgcgt gacataaagc
    30541 aggacgccaa ccggcagacc aatcacaaac ccaaaaaatc cggatacgaa ggtcattgcc
    30601 agcgtttccc atacgccacg aaccagcagc cacatcatcg gctcagacat aacccagtac
    30661 ctctactttt acatggtgtt cctgcagcca ggcaatggcg gcttgcgtat cttgttgtgt
    30721 gccgtgcatt tcagtcagca tgatgccgaa cttaacgcca ccggcgtaat ccatctgcgc
    30781 gctaataatg ttgttgttga cgttgaaacg acgcgcggtt tcagaaagca gtggggcatc
    30841 gaccgattga ccggtaaact ccagacgcag catcggcacg cagtcagtga atggctccgc
    30901 ttgcagacgt tcctggtaat cttccgggat atccagatgc agggtcgact gaataaactt
    30961 ctgcgccagc ggcgttttcg gatgcgagaa cacttcactt accgtgtcct gctcgatcag
    31021 ttcgccatta ctgatgaccg ccacgcaatc acaaatacgc ttcacaacgt ccatttcgtg
    31081 ggtgatcaac agaatcgtca aacccagacg gcggttgatg tctttcagca gttcgagaat
    31141 agaacgtgtc gttgccggat ccagcgcgct ggtggcctca tcacacagca atactttggg
    31201 attgctggct aacgcacggg caatcgccac acgctgtttc tgcccaccgg aaagattcga
    31261 cgggtagcta tcatgcttat cgccaagacc aaccaatgac agcaattccg tcacgcgacg
    31321 tttgatctca tctttcggtg tgttgtccag ctccagcggc agagccacgt tgccaaaaac
    31381 agtacgtgaa gagagcaggt taaaatgctg gaaaatcata ccaatctggc ggcgagcttt
    31441 ggtcaactcg gattctgaca gcgtggtcag ttcctggcca tcgaccagca cgctaccctc
    31501 ggttgggcgc tccagcaggt ttacacaacg tataagcgta ctcttacccg cgcctgaggc
    31561 accgataacg ccataaattt gtccagctgg cacatgcagg ctgacgttgt tcaatgcctg
    31621 gatggtgcgg gtaccctggt ggaacacttt ggtgatattc gaaagtttta tcattgatta
    31681 tttattatcg tcattaagtt agtcgtggca tttcgaatgc ctggaacggg caacgccgtc
    31741 aatgaaatgg atgttaaggc atccagacgt ctaaatcaat caggtttatg cgaagagcac
    31801 tttcttgctg gtcgaaacat gcgatactag cgtcacatgc cttattaagg agctataaaa
    31861 ggtggcgaag agcgtacccg caatttttct tgaccgtgat ggcaccatta atgtcgatca
    31921 cggctatgtc catgagatcg acaactttga atttatcgac ggtgttattg acgccatgcg
    31981 cgagctaaaa aaaatgggct ttgcgctggt ggtagtaacc aaccagtctg gcattgctcg
    32041 cggtaaattt actgaagcac agtttgaaac gctgaccgag tggatggact ggtcgctggc
    32101 ggaccgcgat gtcgatctgg atggtatcta ttattgcccg catcatccgc agggtagtgt
    32161 tgaagagttt cgccaggtct gcgactgccg caaaccacat ccggggatgc ttttatcggc
    32221 acgcgattat ttgcatattg atatggccgc ttcttatatg gtgggcgata aattagaaga
    32281 tatgcaggca gcggctgcgg cgaacgtggg aacaaaagtg ctggtgcgta cgggtaaacc
    32341 tgttacgcct gaagcagaaa acgcggcgga ttgggtgtta aatagcctgg cagacctgcc
    32401 gcaagcgata aaaaagcagc aaaaaccggt gtaatgatta aaagatgagc ggttgaaata
    32461 aaaatgcatt tttccgcttg tcttcctgag ccgactccct ataatgcgcc tccatcgaca
    32521 cggcggatgt gaatcacttc acacaaacag ccggttcggt tgaagagaaa aatcctgaaa
    32581 ttcagggttg actctgaaag aggaaagcgt aatatacgcc acctcgcgac agtgagctga
    32641 aagccgcgtc gc
//
LOCUS       DAPOZO010000056        31850 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPOZO010000056 DAPOZO010000000
VERSION     DAPOZO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.056"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            59..979
                     /gene="malM"
                     /locus_tag="ROI21_002773"
     CDS             59..979
                     /gene="malM"
                     /locus_tag="ROI21_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDX5111674.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            1208..2788
                     /locus_tag="ROI21_002774"
     CDS             1208..2788
                     /locus_tag="ROI21_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HDX5111675.1"
                     /translation="MLGHISKFDGNNSLIKHDVVQGNNKLDLDLLRNFNGVPGLNRDN
                     FIYISDIFLNIKQRNEKNHAINMFREVSISNDIISVKFYRNEEIECACDFLMDKDAQG
                     YTDLSDLDLTSCHFKGDVISKVSFLSSNLQHVTFECKEIGDCNFTTATVDNVIFKCRR
                     LHNVIFIKVSGEYVDFSQSILDTVDFSRSQLTHSNFRECQIRNSKFNNCYLYASHFTR
                     AEFLSDKEISFIKSNLTAVVFDHVRISTGNFKDSVTQRMVLSIDYSDIFGNEYLDGYI
                     NNIIKMIDTLPDDPAILKSVLAVKLVMQLKILNIVNKNFIENMKKIFSHCPYIKDPII
                     RSYIHPDEDNKFDNFMRQNRFSKMHFDTQQMIDFINRFNMNKWLIDQNNNFFIQLIDQ
                     ALRSTNDTIKENAWHLYKEWIRSDDVSPLFIEIEDNLRTFNTNELTRNDNIFILFSSV
                     YDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTPETLFGTKSQDKHAL
                     FPIFTASWRANRIKNKGI"
     gene            3011..3508
                     /gene="ubiC"
                     /locus_tag="ROI21_002775"
     CDS             3011..3508
                     /gene="ubiC"
                     /locus_tag="ROI21_002775"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDX5111676.1"
                     /translation="MSHPALTQLRALRYFKEIPALDTQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            3521..4393
                     /gene="ubiA"
                     /locus_tag="ROI21_002776"
     CDS             3521..4393
                     /gene="ubiA"
                     /locus_tag="ROI21_002776"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDX5111677.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(4548..6971)
                     /gene="plsB"
                     /locus_tag="ROI21_002777"
     CDS             complement(4548..6971)
                     /gene="plsB"
                     /locus_tag="ROI21_002777"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDX5111678.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            7142..7510
                     /gene="dgkA"
                     /locus_tag="ROI21_002778"
     CDS             7142..7510
                     /gene="dgkA"
                     /locus_tag="ROI21_002778"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDX5111679.1"
                     /translation="MANNTTGLTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDVITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            7620..8228
                     /gene="lexA"
                     /locus_tag="ROI21_002779"
     CDS             7620..8228
                     /gene="lexA"
                     /locus_tag="ROI21_002779"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDX5111680.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            8301..9626
                     /gene="dinF"
                     /locus_tag="ROI21_002780"
     CDS             8301..9626
                     /gene="dinF"
                     /locus_tag="ROI21_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDX5111681.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLFDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            9742..9951
                     /locus_tag="ROI21_002781"
     CDS             9742..9951
                     /locus_tag="ROI21_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDX5111682.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(9993..10508)
                     /gene="zur"
                     /locus_tag="ROI21_002782"
     CDS             complement(9993..10508)
                     /gene="zur"
                     /locus_tag="ROI21_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDX5111683.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKSR"
     gene            10826..11782
                     /locus_tag="ROI21_002783"
     CDS             10826..11782
                     /locus_tag="ROI21_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HDX5111684.1"
                     /translation="MSGIISSATEFFNNTLHSNQYEIKDASKEKIKDIYNKSVDYATA
                     NNRDVIIAKIDLFIQKITLNLWTASIKNVSLISRINELKLWVDKCIDDCSQEQLGDRD
                     YIALYVDRAIFHYAISFVCNKVEHQTVSLIDRCTFDVASPNGLPSTIQLFYEKSIDDE
                     SIASLHLQTLGDGFINLENAYHQYDDIRLKPLASLLISCSYGSALADLVAAVDIDEYC
                     EKYITDQVKTLSQQEMKKNLKEMKNLAVAGKLDFNDIVTDMKRQEDEGKRVLKDVTKM
                     HGAAQKLNDFVQSCDPDSTELIQIPNDISNSIYDLFGIELVK"
     gene            12145..13182
                     /gene="dusA"
                     /locus_tag="ROI21_002784"
     CDS             12145..13182
                     /gene="dusA"
                     /locus_tag="ROI21_002784"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDX5111685.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVVDKR"
     gene            13316..13558
                     /gene="pspG"
                     /locus_tag="ROI21_002785"
     CDS             13316..13558
                     /gene="pspG"
                     /locus_tag="ROI21_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDX5111686.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(13724..14707)
                     /locus_tag="ROI21_002786"
     CDS             complement(13724..14707)
                     /locus_tag="ROI21_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDX5111687.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPCGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKTLGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITAGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            14790..16205
                     /gene="dnaB"
                     /locus_tag="ROI21_002787"
     CDS             14790..16205
                     /gene="dnaB"
                     /locus_tag="ROI21_002787"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDX5111688.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            16258..17337
                     /gene="alr"
                     /locus_tag="ROI21_002788"
     CDS             16258..17337
                     /gene="alr"
                     /locus_tag="ROI21_002788"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDX5111689.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            17590..18783
                     /gene="tyrB"
                     /locus_tag="ROI21_002789"
     CDS             17590..18783
                     /gene="tyrB"
                     /locus_tag="ROI21_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDX5111690.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGIYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(19005..19508)
                     /locus_tag="ROI21_002790"
     CDS             complement(19005..19508)
                     /locus_tag="ROI21_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111691.1"
                     /translation="MNISYVNSNKMTSLPVELDALNNKDISYSKDFSYAKDFFLYIET
                     QLKIAKDFCRPGEEVSRSIANKVFHAFIDLVNKIRGKKDCMYICTLCCFAEEVKGDYS
                     HYRTFLFDIGNQYKVKLTQSGKKEFSLTLEFNDTIIESQKVTGNKAKHILEDIEIFYR
                     NKPDTYY"
     gene            19909..20622
                     /gene="aphA"
                     /locus_tag="ROI21_002791"
     CDS             19909..20622
                     /gene="aphA"
                     /locus_tag="ROI21_002791"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDX5111692.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            20733..21149
                     /locus_tag="ROI21_002792"
     CDS             20733..21149
                     /locus_tag="ROI21_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDX5111693.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            21153..21509
                     /locus_tag="ROI21_002793"
     CDS             21153..21509
                     /locus_tag="ROI21_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDX5111694.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(21544..24366)
                     /gene="uvrA"
                     /locus_tag="ROI21_002794"
     CDS             complement(21544..24366)
                     /gene="uvrA"
                     /locus_tag="ROI21_002794"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDX5111695.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            24621..25157
                     /gene="ssb1"
                     /locus_tag="ROI21_002795"
     CDS             24621..25157
                     /gene="ssb1"
                     /locus_tag="ROI21_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDX5111696.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            25316..26563
                     /locus_tag="ROI21_002796"
     CDS             25316..26563
                     /locus_tag="ROI21_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDX5111697.1"
                     /translation="MEKNYRVTSFVMDSGERYCMVVDRASGLPMYYPTLFLTTQIRNK
                     GNAFSTTLAAANNLVLLLRFLDSRGINLEQRLLTKDFFKSHEMDDLRDFAQRKQGRMP
                     SKVLSTLWLDDELTNIVDNGTQHSRLTTFANYLRWYAMHILKMPELDVVEQIKTMATQ
                     IKVRRPSKKSRNSTLQDRSLSDVQLDVLFETIKLESELNPFSIDVQRRNRLMILLLYH
                     LGIRGGELLNIRIQDIDFSSNRILIVRRADERADSRTNEPNAKTRERLIPLAESLAQE
                     LHSFITKDRRKVQNAKKNDYLFVTYKRGPTVGKPISKAAYHKIFSLVKDVSPQLYAIT
                     GHSLRHTWNRKFSERMDAMDGPVSEERQEQIRAYLQGWKAGSGTAATYNKRFIQQKGF
                     EAALALQEGNGTRLPKGIKIDDE"
     gene            26553..28112
                     /locus_tag="ROI21_002797"
     CDS             26553..28112
                     /locus_tag="ROI21_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDX5111698.1"
                     /translation="MMNNILSVLVSELIPAKSKGYSFMLYETMWRLDKNNNISVGMIH
                     DLLDPETLSGFLHTLSFYARNLSAAYTRHIFRCFLDMIRATKATRIDDIMLIGYRSSL
                     DAAHEHELSCVSILLRKWYRLGYPGVSKNVVCLLEGWRLKGGRKGDAVKRLDPLEGPL
                     TDNELQAFNEAVVRAYEKSLITIAELAMALSVSHTGRRSIQISQLRVVDILCGENEKG
                     EPFYVLNVPRAKQRNSFFRESFKPYAMTLELWAVLSAQARNAVTLVENHLGIELQEAD
                     RLQIPLFPDLDVLATIQSPYEFCQLLTTDKLHIAASVITKTLHLIVEESDIRSERTGD
                     LLHINSRRFRYTTGTRAAREGFGELVIAELLDHTDTQNAGVYIKNIPEHVKMLDEAVG
                     FQLAPYAQAFVGVLVDSERDAHRGDDPTSRIRTEMGQGVGSCGEHGFCGANVPIPCYT
                     CMHFQPWLNGPHNEVYKGLLNEREWVKEITGDIQIAAVLDRSIIAVADVIMRCAKRRE
                     ELGEEGLITNG"
     gene            28105..30138
                     /locus_tag="ROI21_002798"
     CDS             28105..30138
                     /locus_tag="ROI21_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDX5111699.1"
                     /translation="MAEIFQFKPKAILKAAENLEAFIVKCRDELTVFGSNLNWEDPVW
                     PNITVFAKLGVITRKPKPQEVQDPVFIDFAKAYFRYQQGHAPTGTKNESKALRAIEAS
                     LLQVNGNANIDGLSITVLDEAAELARQHYSDGSAYSCGREIERLAKFVVENQLVENVV
                     QDWVNPIKRAEDKNKTGREAKKNREEKLPSDIALNALAEIFANDPVHERDIFTTSVFA
                     MLMSAPSRITEVLSLPADCEVFEKDREGIERYGWRFFAGKGYEGDIKWIPTVMVDIAK
                     IAVARVRVLSENARQLAKWIEKHPDKFYRHANCPDVADDVPLTMVQTCMALGFAHHSQ
                     KTCRSCLGSRGLAKEDGVHTLNSLWQHTMKRLPEGFPWFDKDKGIKYSNALFVLNVNQ
                     FHGNRGCLPVELHKPINNFFNNDLTPREVLYGKHATIFDRHGYRTDNGERVKLTSHQA
                     RHLLNTIAQRGGLSNLEIAKWSGRADVKQNRTYNHMTEYELVGMAERLDPTKALFGPV
                     GEVAKHLPVTMQEFNTLEHAAVHVTEYGYCVHDYTMSPCEKFRDCVNCTEQVCIKGDD
                     TEKLDRIKKRLEKSERLFFLADAAVDSGEIGIDRWYQYHKKTVTRLCELVDILENPDI
                     ENGAQIKLRGNDFSQLRRVVAKTSIEAIEQKGKESEEAVMLDDLTTLLGGGLG"
     gene            30131..30550
                     /locus_tag="ROI21_002799"
     CDS             30131..30550
                     /locus_tag="ROI21_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111700.1"
                     /translation="MAKHLDNRDINAIVNLIRGWQEPKLSWSAICEAAEPLVGKLPSR
                     QSLNAHEAIVTAYQTKKEALKGRGPKNPRPASLNIAAARIVNLESEIEELKEENRMYK
                     QQFVIWQYNAYKHGMKEHQLNAQLTKIDRERSDGEKR"
     gene            30602..>31850
                     /locus_tag="ROI21_002800"
     CDS             30602..>31850
                     /locus_tag="ROI21_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HDX5111701.1"
                     /translation="MIDQSFSARNFRRIYDLDRKNKGTLEVEHFPDAYKIGKKITMLK
                     GFIYWLARRLRSGQISTAKFEEKKDKINEHIKLRKEQYNNEVNSKLKIIAQNVGIKGY
                     ALPLTLLPHQVKNKNVYSIGNSVEALFVSRQIKYILSSLYTIKSNNRDLIISRLSALA
                     KEMSPKYIIRADVESFYESINHKSLLDMLHSSPKLSVPPRRVITQLIRKYQTLTGLDK
                     GVPRGVGISSYLSELYMSVVDDKIRALPDVTYYERFVDDLIVIFSPDKGGNTCSYLPQ
                     ITSIINDRALCLNNKTKELDLFTQSNKSFEYLGYKFQLTARDCSIKMSSNKIQKIRSR
                     IDNTFDEYNQSVSKTPKRAAKNILLRMKFLTGNTRLYNSKSKAFVGIYFSNRFITDLS
                     DLNGLDSYLANKINGLQDQKLKKR"
BASE COUNT         8231 a         7277 c         8012 g         8330 t
ORIGIN      
        1 cgggtttagc gcgctattgc ctggctaccg ctgagctcca gattttgagg tgaaaacaat
       61 gaaaatgaat aaaagtctca tcgccctctg tttatcagca gggttactgg caagcgcgcc
      121 tggaatcagc cttgccgatg ttaactacgt accgcaaaac accagcgacg cgccagccat
      181 tccatctgcc gcgctgcaac aactcacctg gacaccggtc gatcaatcta aaacccagac
      241 cacccaactg gcgaccggcg gccaacaact gaacgttccc ggcatcagtg gtccggttgc
      301 tgcgtacagc gtcccggcaa acattggcga actgaccctg acgctgacca gcgaagtgaa
      361 caaacaaacc agcgtttttg cgccgaacgt gctgattctt gatcagaaca tgaccccatc
      421 agccttcttc cccagcagtt atttcaccta ccaggaacca ggcgtgatga gtgcagatcg
      481 gctggaaggc gttatgcgcc tgacaccggc gttggggcag caaaaacttt atgttttggt
      541 ctttaccacg gaaaaagatc tccagcagac gacccaactg ctcgacccgg ctaaagccta
      601 tgccaaaggc gtcggtaact cgatcccgga tattcctgac ccggttgctc gtcataccac
      661 cgatggctta ctgaaactga aagtcaaaac gaactccagt tctagcgtgt tggtcgggcc
      721 tctatttggt tcttccgctc cagctccggt tacggtaggc aacacggcgg caccagctgt
      781 ggctgcaccc gctccggcac cggtgaagaa aagcgagccg atgctcaacg acacggaaag
      841 ttattttaat accgcgatca aaaacgctgt cgcgaaaggt gatgttgata aggcgttaaa
      901 actgcttgat gaagctgaac gcctggggtc aacatctgcc cgttccacct ttatcagcag
      961 tgtaaaaggc aaggggtaat tacgccccac agtgctgatt ttgcaacaac tggtgcgtct
     1021 cctggcgcac ctttttttat gcttcattcc agggatatga gcgatttttt atagtacttc
     1081 acttcttctt cactaagaat atccattatc tcaatgcatt atcagagact cttttccttt
     1141 cgccggtagt gtctggacgc tcaggctact ttttccagtt atttcatttc tgtaatgcag
     1201 aggttttatg ttaggtcata tctcaaagtt tgacggcaat aactctttga taaaacatga
     1261 tgtggtgcaa ggaaataata aattagatct tgatttacta cgtaatttta atggggtgcc
     1321 aggtttaaat agagataact ttatttatat cagtgatata tttttaaata taaaacaacg
     1381 gaacgaaaaa aatcatgcaa taaatatgtt tcgtgaagtc tcaatcagta atgatattat
     1441 aagcgtaaaa ttctatagaa atgaagaaat agaatgtgct tgtgattttc tgatggataa
     1501 agatgcgcag gggtatactg acctgtctga tttggattta acaagttgtc attttaaagg
     1561 tgacgttatt tcgaaggtgt cttttttatc gtcaaatcta caacatgtaa cattcgaatg
     1621 taaagaaata ggggattgca attttactac tgcaacagtt gataatgtca tatttaaatg
     1681 tcgacgttta cacaatgtga tttttatcaa agtgagtggc gaatatgtcg attttagcca
     1741 aagtattctt gatacagttg acttctcgcg gagccagctt actcatagta attttcgtga
     1801 atgtcagatt agaaattcaa agttcaataa ttgttatctt tatgcttcgc acttcaccag
     1861 agcagaattt ctttctgaca aagaaatatc atttattaaa tcgaatttga cagctgtcgt
     1921 gtttgatcat gtgcgaatat cgacagggaa ttttaaagac agcgttacac aacgaatggt
     1981 attatctatt gattactcag atatatttgg aaatgaatat ctcgatggtt atatcaataa
     2041 cattataaaa atgattgata cattgccaga tgatccagcg atattgaaat ccgttctggc
     2101 agtaaaattg gtgatgcaat taaaaattct taatattgtt aataaaaact ttattgagaa
     2161 tatgaagaaa atatttagcc attgtcctta tataaaagat ccaattatac gtagttatat
     2221 ccatcctgat gaagataaca agttcgataa ttttatgcgt caaaatcgat tcagtaagat
     2281 gcatttcgat acccaacaga tgatcgattt tattaacaga tttaatatga ataaatggct
     2341 gattgatcaa aataacaatt tttttatcca acttatcgat caggctctac gatcaacgaa
     2401 tgatacgatc aaagaaaatg cctggcatct ttataaagag tggattcgta gtgatgatgt
     2461 ttcaccttta tttatagaaa ttgaagataa tttaagaacc tttaacacga atgaattaac
     2521 acgaaacgat aatatcttta tcctgttctc ctctgtctat gatgggccag ttatggtggt
     2581 aagctcccag cgcttacatg atatgttgaa tcctacaaaa gataccaatt ggaattccac
     2641 gtatatctac aaatccagac atgagatgtt gcctgttaat cttactccgg aaacactttt
     2701 cggcaccaaa tctcaggata aacatgcgct tttcccaatt tttactgcga gttggcgagc
     2761 taatcgtata aagaataaag gtatttaagt aaaggaaaac atcatcgttc ctggcatcct
     2821 gggcggtgat gtccctatgg ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc
     2881 aacagcgttt taccacttca tgcgattgtt gcgtttttgt tgcgcattag atcacttaat
     2941 ttgctttaca tctcccgtaa acacttttct gcgatacaat gcctttacgt tatgtaacgg
     3001 agagttcggc atgtcacacc ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga
     3061 gatccctgcc ctggatacgc aactgctcga ctggctgttg ctggaagatt ccatgacaaa
     3121 acgttttgaa cagcagggaa aaacggtgag cgtgacgatg atccgcgaag ggtttgtcga
     3181 gcagaatgaa attcccgaag aactgccgct gctgccgaaa gagtctcgtt actggttacg
     3241 tgaaattttg ttatgtgccg atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt
     3301 gtcaacgtta agcgggccgg agctggcgtt acaaaaatta ggtaaaacgc cgttaggacg
     3361 ctatctgttc acatcatcga cattaacccg ggactttatt gagataggcc gtgatgccgg
     3421 actgtggggg cgacgttccc gcctgcgatt aagcggtaaa ccgctgttgc taacagaact
     3481 gtttttaccg gcgtcaccgt tgtactaaga ggaaaaaaat atggagtgga gtctgacgca
     3541 gaataagctg ctggcgtttc atcgcttaat gcgtacggat aagccaattg gcgcgttact
     3601 gctgctctgg ccaacattat gggcgttgtg ggtggcgaca ccgggcgttc cccagctctg
     3661 gatcctggcg gtgtttgtcg cgggtgtctg gctgatgcgc gctgctggtt gcgtggtgaa
     3721 tgattatgct gaccgcaagt ttgatggtca tgttaagcgc acggcgaacc gaccgcttcc
     3781 cagcggcgcg gtaacggaga aagaggcgcg cgcgctgttt gtcgtgctgg tactgatttc
     3841 gtttttactg gtgctgacgc tgaatacgat gaccattctg ttgtcgattg ccgcgctagc
     3901 gctggcgtgg gtgtacccgt ttatgaagcg gtatacccat ctaccgcaag tggtgctggg
     3961 cgcggcgttt ggctggtcga ttccaatggc ttttgctgct gtgagtgagt cggtgccatt
     4021 gagttgctgg ttaatgttcc tcgccaatat tctctgggcg gtggcatacg acacgcagta
     4081 tgcgatggtt gaccgcgatg atgatgtgaa gattggcatt aaatccaccg caatcctgtt
     4141 cggccaatac gataaattga ttattggtat attgcagatt ggcgtactgg cgttgatggc
     4201 gattatcggt gagttaaatg gcttaggctg gggatattac tggtcaattc tggtggctgg
     4261 cgcgctgttt gtttatcaac aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa
     4321 agcatttatg aataataact atgttggtct ggtactgttt ttagggctgg caatgagtta
     4381 ctggcatttc tgatgatgta aaaaagccgg atgatcatcc ggcttccttc tgggttgcct
     4441 gatgcgctgc gcttctcagg cctacacaac atatcgcaat ttattgaatt tgcagatcat
     4501 ggtaggccga ataaggcgtt tgcgccgcat ccggcaattc tccctgatta cccttcgccc
     4561 tgcgtcgcac tctcaatcgt caaacgcacg tctgatgtaa tcaactccgc cagcaactga
     4621 taaaccttca tcgtttctgc cggttcggcg tcgccgctat cgctgatata cccttcatca
     4681 cgcagtgtca gcaccagaga actgaacacc gccttgtcga agaactccgg cgcgttgata
     4741 ccgtgcagca cggagagacg ttgcgcgacg gtgcggctct ctttctccag cgtaccgcgg
     4801 ttgatcgacg ggttggcgct caacagccaa aaagtgatgg cataacgttg cagcgtttcg
     4861 cgcgcgcctg cggccagcag ctgtagcgtg cgagaatgcg ccgggttgat atgcaactca
     4921 tcatcttgca gggtaatcag cccctgacgt tgcatctcat ttgccagcgc atcaataacg
     4981 tccggtaact cgtcgcgatc ccagcgcagg aacagttccg ctttcagcat tgggtaaagc
     5041 acattgacgt gctccatcaa tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc
     5101 gccatcagcg aaggcagcac caacatatgc gcaatgttgt tgcgatagta ggtcatcagc
     5161 accgcttgct cgcgcggcag aatgatgatg tcgccgatag tgtctttctc aacttcaaac
     5221 ttgttcattt gcagcgcgtg atcgataagc tcgctggcgc tggctgaagg aacggtagag
     5281 tccgtggagt aaggtacgtt gcgcatcaga tccaggtagc agttgagttg ctcggttaac
     5341 tgctcgcggg tgagtgagcg ctgacgtgac gccagtagcg cagtacagca caggttcatg
     5401 gcgtttgccg cgcctgcgtt gttaatgcgt accatcagat cggcagcaat attattgacc
     5461 gtcggcgtta accaggccgg acgcaccgct tcgatgggat cgatagattc acgccagtct
     5521 ggtacgtgct ggttaaggta ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc
     5581 tgaccgagat tacgcagctt gcttaaaccg cgcagcatct gcggcaggct ctctttctct
     5641 ttcgtcgcgc cgcgcagttc tttggcgtaa gtacccactt ccatgacgtg ctcataaccg
     5701 atatagatcg gaatcagcgt aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc
     5761 atcgacagcg taccggtttt cggatccagc aaacgccccg tacgggaacg accgccttct
     5821 acgaagtact cgacggaata accacggctg aacagttcgc cgagatactc acggaaaacg
     5881 gtggaataga gcttattgcc tttaaacgta cggcgaataa agaacgcacc cagacggcgg
     5941 aaaatcggcc cggccggcca gaagttcagg ttgatcccgg cggcgatatg cggcggcacc
     6001 agcccctggt gatacagcac gtaagaaagc agcaggtagt ccatgtgact gcggtggcaa
     6061 ggcacataca ccagctcatg accgtcgtgg gccaactggc gaacgcgctc ggcgttatgg
     6121 acgttgatac cctggtaaag tcggttccag gtgaagccca gaatacggtc agtcaggcga
     6181 atcatctcgt aagagaaatt cgccgcaatc tcttccatca gtgcaatcgc gttctgctgc
     6241 gctttttcat gggagatttt tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg
     6301 cgggaggcga gcagcttatt aaacagatcc tgacgagcag gcaaacgtgg gcctacggca
     6361 gccagacgtt gacgggcaaa gtgcatacgc gccacgcgcg ccagtttctg cgcgatagtt
     6421 ttatccgtgc cgtgttcatc cgccatacgg cgcagcgaaa ctgacggcga gaaacgcaca
     6481 aaactgtcgc gaccgagcca cagtacagcg aaaaatttct gtacgccgtt aagcatacgc
     6541 agcggcgggt ttacttcgcc tttttcacgc cccggcgcgc gaccaaacat caccgatact
     6601 ggcaccatct gcacatccag atttgggttg ctacggtgca agtcgagata gtcgtggaac
     6661 agcttaatag actcttcttt cggcgtgtaa taggtgaaca cacgcggccc gccgtgaatg
     6721 aacacatagc gcggcagtag cgtgccgtcg atttccagcg gctctaacgg gtcaggcagg
     6781 tcatgtgcca gacactgggc gcgcaacgtc agcaagtctg ctttcgagtt gtacggtaaa
     6841 acgtacataa ttggacgaga ggtatccagc cccagttccg gggcaggatc tgccggaata
     6901 gacttgcttt ttaccaggat gcttaatggt aaattcagta atttgtagta aattcgtggc
     6961 cagccggaca taaacgatgt aaagcctctg gttaataatg caaatgcgcg gcaaggatag
     7021 cagaaagtca tgggaaattc tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc
     7081 cgaatcatcg gatttaacgg tacactgata ttgacgctca taatgtaaaa aggttctttc
     7141 aatggccaat aataccactg gactcacccg aattatcaaa gctgctggct attcctggaa
     7201 aggtttacgc gctgcatgga tcaacgaagc ggcattccgt caggaaggcg tagcggtatt
     7261 gttggcggtg gtcatcgcct gctggctgga tgtggacgta attacccgcg tgctgcttat
     7321 cagctccgtg atgctggtga tgattgtgga aatcctcaat agcgccatcg aagcagtggt
     7381 tgaccgaatt ggctctgaat accatgagct atccggacgc gctaaagata tgggatccgc
     7441 cgcggtgctg attgcgatta tcgtcgccgt gattacctgg tgcatcctgt tatggtcgca
     7501 ttttggataa cccttccaga tttcgataaa tctctggttt attgtgcagt ttatggttcc
     7561 aaaatcgcct tttgctgtat atactcacag cataactgta tatacaccca gggggcggaa
     7621 tgaaagcgtt aacggccagg caacaagagg tgtttgatct catccgtgat cacatcagcc
     7681 agacaggtat gccgccgacg cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa
     7741 acgcggctga agaacatctg aaggcgctgg cacgcaaagg cgttattgaa attgtttccg
     7801 gcgcatcacg cgggattcgt ctgttgcagg aagaggaaga agggttgccg ctggtaggtc
     7861 gtgtggctgc cggtgaaccg cttctggcgc aacagcatat tgaaggtcat tatcaggtcg
     7921 atccttcctt attcaagccg aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga
     7981 aagatatcgg cattatggat ggcgacttgc tggcagtgca taaaactcag gatgtacgta
     8041 acggtcaggt cgttgtcgca cgtattgatg acgaggttac cgttaagcgc ctgaaaaaac
     8101 agggcaataa agtcgaactg ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc
     8161 ttcgtcagca gagcttcacc attgaagggc tggcggttgg agttattcgc aacggcgact
     8221 ggctgtaaca tatctctgag accgcgatgc cgcctggcgt cgcggtttgt ttttcatctc
     8281 tcttcatcag gcttgtctgc atggcattcc tcacttcatc tgataaagca ctctggcatc
     8341 tcgccttacc catgattttc tccaatatca ccgttccgtt gctgggactg gtcgatacgg
     8401 cggttattgg tcatcttgat agtccggttt atttgggcgg cgtggcggtt ggtgcaacgg
     8461 cgaccagctt tctctttatg ttgttgctgt ttttacgcat gagcaccacc gggctgactg
     8521 cgcaggctta tggtgccaaa aatcctcagg cattagcccg agcgctggtg caaccgttgc
     8581 tgttggcgtt gggggctggg gcgttaattg cgctgctgcg tacgccgatt atcgatctgg
     8641 cgctgcatat tgttggcggc agcgaagcgg tgctagaaca ggcgcggcgc tttcttgaaa
     8701 tccgctggtt aagcgcaccg gcgtcgctgg cgaatctggt attacttggt tggttactcg
     8761 gcgtgcaata tgcccgtgcg ccagtaattt tgttagtggt cggcaatatc ctcaacattg
     8821 tgctggatgt ctggctggtg atggggctgc atatgaacgt gcagggcgcg gcgctggcga
     8881 cggttattgc ggaatatgca acattgctga ttggtctgct aatggtgcgt aaaatcctca
     8941 aactacgcgg aatctccggc gaaatgctga aaactgcctg gcgaggaaat ttccgtcgct
     9001 tactggcgct taaccgcgat atcatgctgc gctcgctgtt gttgcaactc tgtttcggcg
     9061 cgatcaccgt acttggcgcg cgactgggga gtgacattat cgctgttaac gcggttctga
     9121 tgacgctact cacctttacc gcctatgcgc tggatggttt tgcctacgcg gttgaagcac
     9181 attctggtca ggcgtacggt gcgcgcgacg gtagccagtt actggatgtc tggcgggcag
     9241 cgtgccgcca gtcggggatt gtagcgttac tgttttcggt ggtttatttg ctggcagggg
     9301 aacacatcat tgcattgctg acgtcgttaa cccagattca gcagctggcc gaccgctatc
     9361 ttatctggca ggtgattttg ccgttggtcg gcgtctggtg ttatctgttc gacggcatgt
     9421 ttataggtgc aacgcgcgcc gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt
     9481 ttgcgctgac gctccttacg ctgccgtggc tgggtaatca tggtttgtgg ctggcattaa
     9541 ccgtctttct ggctttgcgc gggctttctc tggcggctat ctggcggcgt cactggcgca
     9601 acggtacctg gtttgccgca acgtgacggt taaaaattct gaataaataa tcctaagcca
     9661 aattgctgac tacacttaat ctcacgttca gaagaaaagt gaacgtactc tcattcacaa
     9721 cctaacgatg aggtcttgat tatgaataaa gatgaagccg gcggtaactg gaaacagttt
     9781 aaaggtaaag tgaaagagca atggggcaaa ctgaccgatg atgatatgac gatcattgaa
     9841 ggtaaacgtg atcaactggt cggtaaaatc caggaacgtt atggttatca gaaagatcag
     9901 gcagaaaaag aggtcgtgga ttgggaaacc cgcaatgagt atcgctggta attaatccct
     9961 cctgcccgac gtgtacaagg atgtacgccc tcttaacgtg atttcttttt cacctgtaca
    10021 gagtgatcat gctggcactg ttcaggatga cgacacgctt ccacttccac gcatgccgca
    10081 cagagtccat gtgcttcaat cacattatgc cgcagggcaa accccatttt ggccgccagc
    10141 gtatgcataa tgtcttccac gccttctgca cattcttctt tcactgcgcc gcagcgatcg
    10201 caaataaaca tggctgacgt atgcgtgggc tggtcgaaca gatgacagag cacataactg
    10261 ttggtggatt ccaccttatg cacaaaacct tgctcaagaa gaaaatccag cgcgcgataa
    10321 accgttggcg gcttggcttg cggttcagct tcgcgcagca aatcaagcag atcataagcg
    10381 ctgatagcgc catcctgcag actcatcagg cgcaacactt ccaggcgctg tggggtcagg
    10441 cgcacattac gctgcgcaca gattttttca gcctgcgcta ataactcctg cgttgtggtc
    10501 ttttccatcg ggcacctcaa agtgggggtt aagaatgtcc ttactttacc atgttccagg
    10561 aaaaacaccg agaaccctta ttgttgccgt aatgttgatt ttctgttttg tacgtaaggt
    10621 gttgttttgc ctggtagtac cattatcaac acgataataa ttaataattc atttttaaat
    10681 aaattgcttc aagttaaatc gctatttatt tagctgggtt tgatcgcata cctgtacttc
    10741 agagattatg agcgattttt gatagtgagc agatggcaaa agaattataa ggaggacacg
    10801 gtacgacaaa aaaggtggat gaatcatgtc aggtataatt tcaagtgcaa cagaattctt
    10861 caataataca ctccattcaa atcaatacga aattaaagat gcgagcaaag aaaaaataaa
    10921 ggatatatat aataaatcgg ttgattatgc aactgcgaat aatcgcgatg tgattattgc
    10981 aaaaatagat ttatttattc aaaaaatcac ccttaattta tggacagcaa gtatcaaaaa
    11041 tgtgtcgtta atcagcagaa taaatgaact caaactgtgg gtcgataagt gtattgatga
    11101 ctgctctcaa gagcaattag gcgatcgtga ttatatcgct ttatatgtag acagggcgat
    11161 ttttcattat gcgattagtt ttgtctgtaa taaagtagaa catcagacag taagtctcat
    11221 agacagatgt acttttgatg tagcaagccc gaatggactc ccatcaacta ttcaactatt
    11281 ttatgaaaaa tctattgatg atgaatctat agcttcgtta catctacaaa ctttaggtga
    11341 tggttttata aatttagaaa atgcatatca tcaatatgac gatattcgct taaaaccctt
    11401 ggcttccctt ttaatttcat gttcatatgg tagtgcatta gcagatctgg tagctgcagt
    11461 ggatattgat gagtattgcg aaaaatacat aacagatcag gtgaaaacgt tatctcaaca
    11521 agagatgaaa aaaaatctga aggagatgaa aaatctggca gtcgcaggga aactggattt
    11581 caatgacatt gtaactgata tgaaacgtca ggaagacgaa ggaaaacgag tcctaaaaga
    11641 cgttacaaaa atgcatggtg cggcgcagaa attaaatgac ttcgtacaat cgtgtgaccc
    11701 agattcaacg gaattgattc aaattccaaa cgatatctct aattcaattt atgatctatt
    11761 tggtattgaa ctagtaaaat aatatgtgaa gttcttcgat ggtatggaag gtattacata
    11821 aaagaaccca atgcttattg ggttcttttt ttctctatca gtgctattag cagggagaga
    11881 tatcaccgta gtttaatgtg tgatttttat ttatcgtcga acctggattg tttatcattg
    11941 gccttaacaa agctaacggc taataagatt atttccatca cttcgtgaga gcttcatgcc
    12001 ttgagaggat ctcaattttc ttttgcaatg agacaggcgc ttcctgttgt tatggtatag
    12061 taccccgcta ttgagcctcc tgaacagtga tgctgaataa cataacccca tgatatatcg
    12121 ataaaataat ctctacattt gaaaatgcac ggtaattctg aaatgcaaaa aatcaaccaa
    12181 accagcgcaa tgcctgaaaa aactgacgtt cactggagtg gtcggtttag cgttgcacca
    12241 atgctcgact ggacggacag acattgccgc tatttcttgc gtctgctttc ccgcaatacg
    12301 ttgctgtata ccgaaatggt gactacaggg gcgattattc acggtaaagg tgattacctg
    12361 gcgtacagtg aagaagaaca tccggtagca ttgcaactgg gcggtagcga tccggcggcg
    12421 ctggcgcagt gtgcaaagct ggcagaagcg cgcggatatg atgagatcaa cctgaatgtc
    12481 ggctgcccgt ctgaccgggt gcagaacggc atgtttgggg cgtgtctgat gggtaatgcg
    12541 cagctggttg ccgactgcgt gaaagcgatg cgcgatgtgg tgtcgattcc ggtgacggtg
    12601 aaaacgcgta ttggcatcga cgaccaggac agctatgaat ttctctgcga tttcatcaac
    12661 accgtttccg gcaaaggcga gtgtgagatg ttcatcatcc acgcacgtaa agcctggctt
    12721 tcggggttaa gcccgaaaga aaaccgtgaa atcccgccgc tcgattatcc gcgtgtgtat
    12781 caactgaagc gtgactttcc gcatctgaca atgtcgatta acggtggcat caagtcgctg
    12841 gaagaggcta aagcgcattt gcaacatatg gatggcgtga tggtcgggcg cgaagcgtat
    12901 cagaatccgg gtattctggc ggcggtagac cgagagatct ttggttcctc ggataccgat
    12961 gccgatccgg tggcggtagt gcgcgccatg tatccgtaca ttgagcgtga actcagccag
    13021 gggacgtatc ttggccatat tacccggcat atgctgggct tgttccaggg tattcctggc
    13081 gcgcggcagt ggcggcgtta tttaagtgaa aatgcccata aagcgggtgc agacattaat
    13141 gtgctggaac acgcgctcaa actggtggtg gataagcgtt aacttttcac caaaaaatag
    13201 tcaaattcac cacgccctgc gcaccgtcgc ggggcgtttt gctgttaaat caatagatta
    13261 tttttggcat gattcttgta atgccagcaa gagatttcat atttgggaga gcatcatgct
    13321 ggaactactt tttgtgattg gcttttttgt catgttgatg gtcaccggcg tttcgttgct
    13381 gggcattatc gccgcgctgg ttgtggcgac ggccattatg ttcctcggcg gtatgctggc
    13441 attgatgatt aagttgctgc cgtggttact cctggcgatt gcggtggtgt gggttattaa
    13501 ggcgattaaa gcaccaaaag tgccgaaata tcagcgttat gaccgctggc gttactaagg
    13561 gattgtgcgg atgatcacaa cctaaggttt tatccttaga acaaaatagg aattgataat
    13621 caaatctgtc actattgcgc ctctaacaga ttcatcgtgc tgtaccctac atacagccga
    13681 actataaaaa gaaagggctt cccaggtgga agccctattt cttttatgga atcaacaggc
    13741 tggaaccttg cgtcgcccgg ctttccagaa tctcatgcgc acgctgcgca tccttcagcg
    13801 gatatttctg ctgctcggcg acatcgacct taatcacacc gctggcaatc aaagagaaca
    13861 gttcattact ggcctcggtt aattcctccc gcgtggtgat atagccttgc agggaagggc
    13921 gtgtcacata caacgagcct ttctgattga gaatgcctaa gttcacaccg gtaaccgcac
    13981 ctgatgagtt gccaaagctc accattaagc cgcggcgttg caggcaatcc agcgaccgtt
    14041 cccaggtgtc tctgcccacg gaatcgtaca ccacgcgcac tttcttgcca gcggtgatct
    14101 cttttaaccg ctcgaccaga ttctcttcgc gatagttaat aacctgccat gcgcccgctt
    14161 ttagcgcact ctgcgctttt tgcgcggttc ccacggtgcc gataagtttc gccccaaggg
    14221 tttttgccca ctggcaagca attaagccaa cgccgccagc cgctgcgtgg aacaggaact
    14281 gctcatcggg tttaatttca taggttttgc gcagcagata ataaaccgtt aagcctttca
    14341 ggaaggatgc cgcagcttgc tcaaaagaaa ttgccgcagg cagaatcgcc gctttatccg
    14401 cattaatgtt atgcacagag ctgtaagcgc ctaacgccga ctgcgcatag actacacgat
    14461 cgcctgcctt aatatgcttt acaccactgc cgactttact cacgatgcct gccgcttcgg
    14521 tgcctaatcc gcagggtagt gatggcggcg ggtaaaggcc gctgcggata tacgtgtcga
    14581 taaaattgat gccgatggct ttattttcga cctggatttc attctccgcc ggatcggcag
    14641 gagtgaactc tacggcttga agtacttccg ggccaccgtg cttgtgaaat tcaattcgtg
    14701 ttgccatgtg tcctccagaa cgtatcgtca gggtctgctt catatggtaa agtttcgacc
    14761 cattctttat ctcggtaact ccattcacta tggcaggaaa taaacccttc aacaaacagc
    14821 aggctgaacc ccgcgaacgc gatccacaag ttgccgggct gaaagtgcct ccgcactcga
    14881 tcgaagcgga gcagtcggtg ttgggcggtt taatgctgga taacgaacgc tgggatgatg
    14941 tagccgagcg tgtggtggca gacgactttt acacccgccc acaccgtcat atctttactg
    15001 aaatggcgcg tttacaggaa agcggtagtc ctatcgatct gattaccctt gcggaatcgc
    15061 tggaacgcca ggggcaactc gatagcgtcg gtggtttcgc ttatctggcg gagctgtcaa
    15121 aaaatacgcc aagtgcggcg aacatcagtg cttatgctga catcgtgcgt gaacgtgccg
    15181 ttgttcgcga gatgatttcg gttgcgaatg agattgccga agccggtttt gatccgcagg
    15241 ggcgcaccag cgaagatctg ctggacctgg ctgaatcccg cgtctttaaa attgccgaaa
    15301 gtcgtgcaaa caaagacgaa gggccgaaga acatcgctga tgtgctcgac gcaaccgtgg
    15361 cgcgtattga gcagttgttt cagcagccac acgatggcgt taccggagta aacaccggtt
    15421 atgacgatct caacaaaaaa accgctggct tgcagccgtc ggatttgatc atcgtcgccg
    15481 cgcgtccgtc gatgggtaaa accacatttg cgatgaacct cgtcgaaaac gcggcgatgt
    15541 tgcaggataa acctgtactt atcttctcgc tggagatgcc atcagaacag attatgatgc
    15601 gttctctggc gtcgctgtcg cgcgttgacc agactaaaat ccgtaccggg cagctcgatg
    15661 atgaagactg ggcacgtatt tccggcacca tgggtatttt gctcgaaaaa cgtaatatct
    15721 atatcgatga ttcctccggc ctgacgccaa cggaagtgcg ttcccgcgca cgccgtattg
    15781 cccgtgaaca cggcggcatc gggcttatca tgatcgacta cctgcaactg atgcgcgtac
    15841 cggcgctttc cgataaccgt acgctggaaa ttgcagaaat ctcccgctcg ctgaaagcac
    15901 tggcgaaaga attgaacgtg ccggtggtgg cgctgtccca gttgaaccgt tctctggaac
    15961 aacgtgccga caaacgcccg gtcaactccg acctgcgtga atctggctct atcgagcagg
    16021 atgcggactt gatcatgttt atctatcgtg atgaggtgta tcacgaaaac agtgatttaa
    16081 aaggcatcgc ggaaattatt atcggtaaac aacgtaacgg cccaatcggg acggtacgcc
    16141 tgacctttaa cggtcaatgg tcgcgcttcg acaactatgc ggggccgcag tacgacgacg
    16201 aataataatt attttatgaa ttaggtaatt aaagcaaaca cttatcaagg aacacaaatg
    16261 caagcggcaa ctgttgtgat taaccgccgc gctctgcgac acaacctgca acgtcttcgt
    16321 gaactggctc ctgccagtaa aatggttgcg gtggtgaaag cgaacgctta tggtcacggt
    16381 cttcttgaga ccgcgcgaac gctccccgat gctgacgcct ttggcgtagc ccgtctcgaa
    16441 gaagctctgc gactgcgtgc ggggggaatc accaaacctg tactgttact cgaaggcttt
    16501 tttgatgcca gagatctacc gacgatttct gcgcaacatt ttcataccgc cgtgcataac
    16561 gaagaacagc ttgctgcgct ggaagaggcc agcctggacg agccggttac cgtctggatg
    16621 aaactcgata ccggtatgca ccgtctgggc gtaaggccgg aacaggctga ggcgttttat
    16681 catcgcctga cccagtgcaa aaacgttcgt cagccggtga atatcgtcag ccattttgcg
    16741 cgcgcggatg aaccaaaatg cggcgcaacc gagaaacaac tcgctatctt taataccttt
    16801 tgcgaaggca aaccaggtca acgttccatt gccgcatcgg gtggcatttt actctggcca
    16861 cagtcgcatt ttgactgggt gcgcccgggc attattcttt acggtgtatc tccgctggaa
    16921 gatcgctcca ccggtgccga ttttggctgt cagccagtga tgtcactaac ctccagcctg
    16981 attgccgtgc gtgagcataa agccggagaa cctgttggtt atggtggaac ctgggtaagc
    17041 gaacgtgata cccgtcttgg cgtagtcgcg atgggctatg gcgatggtta tccgcgcgcc
    17101 gcgccgtccg gtacgccagt gctggtaaac ggtcgcgaag tgccgattgt cgggcgcgtg
    17161 gcgatggata tgatctgcgt agacttaggt ccacaggcgc aggacaaagc cggggacccg
    17221 gtcattttat ggggcgaagg tttgcccgta gaacgtatcg ctgaaatgac gaaagtaagc
    17281 gcttacgaac ttattacgcg cctgacttca agggtcgcga tgaaatacgt ggattaatcg
    17341 ttctgtaata tttgattgtt tgtgccggat gcggcgtgaa tgccttatcc ggccaataaa
    17401 atcctaccaa ttcaataagt tgatattctt tcatgctctt atgaaggccg tgcctctggc
    17461 ggatgtacgt ttgtcatgag tctcactctg ttgctaattg ccgttcgccc ctgaacatcc
    17521 actcgatctt cgccttcttc cggtttattg tgttttaacc acctgcccgt aaacctggag
    17581 aaccatcgcg tgtttcaaaa agttgacgcc tacgctggcg acccgattct tacgcttatg
    17641 gagcgtttca aagaagaccc tcgcagcgac aaagtgaatt taagtatcgg tctgtactac
    17701 aacgaagacg gaattattcc ccaactgaaa gccgtggcgg atgcggaagc gcgcctgaat
    17761 gcgcagcctc atggcgcttc gctttattta ccgatggaag ggcttaatag ctatcgccat
    17821 gccattgcgc cgctgctgtt tggtgcggac catccggtac tgcaacaaca gcgcgtagca
    17881 accattcaaa cccttggcgg ctcaggggca ttgaaagtgg gcgcagattt cctgaaacgc
    17941 tacttcccgg aatcaggcgt ctgggtcagc gatcctacct gggaaaacca cgtagcaata
    18001 ttcgccgggg ctggattcga agtgagtact tacccctggt atgacgaagc gactaacagc
    18061 gtgcgcttta atgacctgtt ggcgacgctg aaaacattac ctgcccgcag tattgtgttg
    18121 ctgcatccat gttgccacaa cccaacgggt gccgatctca ctaatgatca gtgggatgcg
    18181 gtgattgaaa ttctcaaagc ccgcgagctt attccattcc tcgatattgc ctatcaagga
    18241 tttggtgccg gtatggaaga ggatgcctac gccattcgtg ccattgccag cgctggatta
    18301 cccgctctgg tgagcaattc gttctcgaaa attttctccc tttacggcga gcgcgttggc
    18361 ggactttctg ttctgtgtga agatgccgaa gccgctggcc gcgtactggg gcaattgaaa
    18421 gcaacagttc gccgcaacta ctccagcccg ccgaattttg gtgcgcaggt ggtggctgcg
    18481 gtgctgaatg acgaggcatt gaaagccagc tggctggcgg aagtagaaga gatgcgtact
    18541 cgcattctgg cgatgcgtca ggaactggtg aaggtattga gcacagagat accagaacgc
    18601 aatttcgatt atctgcttaa tcagcgcggc atgttcagtt ataccggttt aagtgccgct
    18661 caggttgacc gactacgtga ggagtttggt atttatctca tcgccagcgg tcgcatgtgt
    18721 gtcgccgggt taaatacggc aaatgtacaa cgtgtagcaa aggcgtttgc tgcggtaatg
    18781 taatgcagga aagcaggctg gagttatcca gcctgcagtg aaattaaact gtcgtcgctt
    18841 tcactcttgc tttatagatg atttttttga tgccatcgtt ctacgtgaga ggtaataaaa
    18901 cgttgttagt tctaacttgt taagtttatc ccaatgttct ggagtgcttt tccctgtttt
    18961 accgtgtgga gtaataattt ctgcacatct aaccattatt aaatttaata ataagtatcg
    19021 ggtttgttac gatagaatat ttctatatcc tcaagaatat gctttgcctt attgcctgtg
    19081 actttctgag attcaattat agtatcgtta aattctaatg ttaaagagaa ctcttttttt
    19141 ccgctttgtg taagtttaac cttatattga ttaccaatat caaataaaaa ggtcctgtaa
    19201 tgagaataat cacctttaac ctcctcggca aaacagcaaa gtgtgcaaat atacatacaa
    19261 tctttcttgc ctctgatttt gttaactaaa tcaataaatg catgaaaaac tttatttgca
    19321 atagatcttg atacttcctc tcctggtcta caaaaatctt ttgcaatttt taactgtgtt
    19381 tctatatata aaaagaaatc tttggcataa gaaaaatctt tggaataaga gatatcttta
    19441 ttatttagtg catctagctc aacaggtaaa gatgtcattt tgttgctatt cacatatgat
    19501 atattcatat catcatctcc ttcgagagta ataaaattag tcaccattgt agggtagggt
    19561 tctggtcaac cagaaatcat ctttataaac ttcgattgtt tttgtaatgc tgtatcatta
    19621 agttcattaa atcgtacagc agataaatgt tctatcaaat ttcgctcatt tgcagaggat
    19681 tcatcataat aaacgtaaaa ttaatgtatc cttcattgag gaataaacat ttttttacaa
    19741 aaaaacgaca ggaacgtatt tactgggtta aatataatca tcctgctttt catcacaaaa
    19801 accgcagata atccttcctt tcccctgctg ctggcgttat ggtcagatgg tttttgcaac
    19861 aaatctcaca ataaaaagtt tcaacatact gactatttag ggaaaaatat gcgcaagatc
    19921 acacaggcaa tcagtgccgt ttgcttattg ttcgctctaa acagttccgc tgttgccctg
    19981 gcctcatctc cttcgccgct taaccctggt actaacgttg ccaggcttgc tgaacaggca
    20041 ccaattcatt gggtttcggt cgcacaaatt gaaaatagcc tcgcagggcg tccgccaatg
    20101 gcggtggggt ttgatatcga tgacacggta cttttttcca gtccgggctt ctggcgcggc
    20161 aaaaaaacct tctcgccaga aagcgaagat tatctgaaaa atcctgtgtt ctgggaaaaa
    20221 atgaacaatg gctgggatga attcagcatt ccgaaagagg tcgctcgcca gctgattgat
    20281 atgcatgtac gccgcggtga cgcgatcttc tttgtgactg gtcgtagccc gacgaaaaca
    20341 gaaacggttt caaaaacgct ggcggataat tttcatattc ctgccaccaa tatgaatccg
    20401 gtgatctttg cgggtgataa accagggcaa aatacaaaat cgcaatggct gcaggataaa
    20461 aatatccgaa ttttttatgg cgattccgat aacgatatta ccgccgcacg cgatgtcggc
    20521 gctcgtggta tccgcattct gcgcgcctcc aactctacct acaaaccctt gccacaagcg
    20581 ggtgcgtttg gtgaagaggt gatcgtcaat tcagaatact gacagcgcgg gagagcgtga
    20641 cactctcccg aatgctgttt ttttaatcac acctttatcc tttctctgtc ttgctgcaaa
    20701 ctgattaaga gagttttatc aaggagcagc acatgtggta tcaaaagacg ctcacgctta
    20761 gcgccaaatc tcgtgggttt catctggtaa cggatgaaat tctgaatcag ctggctgata
    20821 tgccgcgcgt taacatcggt ttactgcatc tgttgttgca acatacctcc gcctctctga
    20881 cacttaatga gaactgcgat cccaccgtac gccacgacat ggagcgtttt ttcctccgca
    20941 cagttcccga caacggaaat tatgagcatg actatgaggg agcagacgat atgccttctc
    21001 atatcaaatc ctcaatgctg ggaacatcgc ttgtattgcc ggtgcataaa gggcgtattc
    21061 agaccggcac ctggcaaggc atctggctgg gggaacatcg catccacggc ggatcgcgtc
    21121 gcattatcgc gacactacaa ggggagtaaa aaatgaccat ttcggagttg ctacaatatt
    21181 gcatggcaaa accaggcgca gaacagagcg tgcataacga ctggaaagcg acgcagatca
    21241 aagtggaaga tgtactgttt gcgatggtga aagaagtaga aaatcgcccg gcggtttcgc
    21301 tgaaaaccag cccggagctg gcggagctgc tacgtcagca gcacagcgat gtgcgtccaa
    21361 gccgccatct gaataaagcg cactggagca ccgtgtatct cgacggatcg ctaccggatt
    21421 cgcaaatcta ttatctggtg gatgcgtctt atcagcaggc ggtgaatttg ctgccggaag
    21481 aaaaacgtaa attgctggtg caactctgaa aggaaaaggc cgctcagaaa gcggccttaa
    21541 cgattacagc atcggcttga ggaagcgtgc cgtatgcgaa gcttcgcact ccgcgacggt
    21601 ttctggcgta ccggagacga ggatttcgcc gccgccactg ccgccttccg gtcccaggtc
    21661 gacaatccag tcagcggttt tgatcacgtc gagattgtgc tcaatcacca caatggtgtt
    21721 gccctgatcg cgcagtttat gcagtacgtc gagcagttgc tgaatatcgg cgaagtgcag
    21781 accggtggtc ggctcatcaa gaatatacag cgtctgcccg gtgccgcgtt ttgacagctc
    21841 acgcgccagc ttcacgcgct gggcttcacc accagaaagt gtggttgcgg actgccccag
    21901 gcgaatgtac gtcagaccaa cgtccatcaa cgtttgcagc ttacgcgcca gtgcaggtac
    21961 ggcatcaaag aactcacgcg cctcttcgat ggtcatatcc agcacttcgt ggatggtttt
    22021 gcctttgtac ttaatctcca gcgtttcacg gttatagcgt ttgcctttgc actggtcgca
    22081 cggtacgtag atatccggca ggaagtgcat ctccactttg atcacaccgt cgccctgaca
    22141 ggcttcgcag cgtccgccac ggacgttaaa gctgaaacgt cctggcgtat aaccacgcgc
    22201 acgggattcc ggtacgcccg caaacagttc gcgcacaggc gtaaacacgc cggtataggt
    22261 cgccgggtta gaacgcggag tacgaccaat tgggctttgg tcgatatcga tcactttatc
    22321 gaaatgctcc agcccctgaa tatcgcgata cggtgccggt tcggcgatgg tcgcaccatt
    22381 caactggcgt tgggcaatcg ggaacagtgt gtcgttaatc agcgtcgatt taccggaacc
    22441 tgaaacccct gtgatgcagg taaacagacc gactggcagc gtcagcgtca cgtctttcag
    22501 gttattacca cgtgcgcctg tcagcttcag cactttttcc ggattcgccg gaacgcgttt
    22561 cttcggcact tcaatcttgc gtttgccgct catgtactgc ccggtcaacg actcaggcac
    22621 tgccataatc gcttccagtg gaccttctgc gaccacttca ccgccgtgta cacctgcgcc
    22681 cgggccaatg tcgatcacat ggtcagcggc gcgaatcgcg tcttcgtcgt gctccaccac
    22741 aatcacggta ttaccgagat cgcgcagatg gataagcgta cccaacaggc gctcgttatc
    22801 gcgctggtgc aggccgatag acggctcgtc cagcacgtac ataacgccaa ccaggcccgc
    22861 accaatctgg ctcgccagac ggatacgctg ggcttcaccg ccagaaagtg tctctgccga
    22921 gcgggaaagt gtcaggtaat tcaggcctac gttaacgagg aatttcaggc gatcgccaat
    22981 ctctttaaga attttttccg caatcttcgc tcgctgacct gcgagtttga gattgttgaa
    23041 gaattccatc gcatgaccga tgctcatgtc ggagatagca ggcagcggcg tattctcgac
    23101 atacacatgg cgcgcttctc gacgcagacg cgtcccttcg cagctggcgc acgggcgatt
    23161 gctgataaac ttggctaatt cttcgcgtac cgcactggat tccgtctctt tataacggcg
    23221 ctccatattg tgcagcacgc cttcgaacgg atgacgacga atggaggtat cgccacgatc
    23281 gttcatgtat ttgaattcaa tgttttcttt gccagaaccg tacaacacca ctttatgcac
    23341 gttcgcgctc aggctgcccc acggcgcttc gacgtcgaac ttatagtgat ctgccagcga
    23401 tttcagcatc tggaagtaat agaagttgcg gcgatcccag cctcggatcg caccgccagc
    23461 cagcgacagt tccggattct ggatcacgcg gtcaggatcg aaatattgct gcacgccaag
    23521 gccgtcacag gtcgggcagg cccctgccgg gttgttaaac gaaaacagtc gcggctccag
    23581 ttcacgcata ctgtagccgc aaattgggca ggcgaaatta gcggagaaca gcaactcttc
    23641 cgctttcggg tcgtccatat ccgcaactac cgcggtacca ccggaaagct ccagcgcggt
    23701 ttcaaacgac tcggcaagac gttgggtaag atcgtcacgc accttgaagc gatcaaccac
    23761 cacttcaatg gtatgtttct tttgcagttc cagtttcggc ggatcggaaa gatcgcagac
    23821 ttcgccatca atacgagcac ggatgtaacc ctgacttgcc aggttctcca gcgttttggt
    23881 gtgttcgcct ttgcgctctt taatgattgg cgcgagcagc atcaggcgtt taccttccgg
    23941 ctgggacaac acgttatcca ccatctgact gacggtttgc gccgccagcg ggacgtcgtg
    24001 gtccgggcag cgcggctcac cgacgcgggc gaacaacaaa cgcaaatagt cgtggatttc
    24061 ggtgattgtc cccaccgtag aacgcgggtt atgagacgtc gatttctgct caattgagat
    24121 ggcaggagaa agcccctcaa tatgatcgac gtccggcttt tccatcagtg acagaaactg
    24181 ccgcgcgtag gcggaaaggg attcaacgta acggcgctgc ccttcggcat ataaggtgtc
    24241 gaaagcgagc gaggatttgc cagaacccga aagcccggtc acgacaatga gcttgtcgcg
    24301 ggggataacg aggttgatgt ttttgagatt atgggtgcgg gcgccccgaa cttcgatctt
    24361 atccattcac ctttcccgga ttaaacgctt ttttgcccgg tggcatggtg ctaccggcga
    24421 tcacaaacgg ttaattatga cacaaattga cctgaatgaa tatacagtat tggaatgcaa
    24481 tacccggagt gttgtgtaac aatgtctggc caggtttgtt tcccggaacc gaggtcacaa
    24541 catagtaaaa gcgctattgg taatggtaca atcgcgcgtt tacacttatt cagaacgatt
    24601 ttttttcagg agacacgaac atggccagca gaggcgtaaa caaggttatt ctcgttggta
    24661 atctgggtca ggacccggaa gtacgctaca tgccaaatgg tggcgctgtt gccaacatta
    24721 cgctggctac ttccgaatcc tggcgtgaca aagcgaccgg cgagatgaaa gagcagactg
    24781 aatggcaccg cgttgtgctg ttcggcaaac tggcagaagt agccagcgaa tatctgcgta
    24841 aaggttctca ggtttatatc gaaggtcagc tgcgtacccg caaatggacc gatcaatccg
    24901 gtcaggatcg ctacaccaca gaagtcgtgg tgaacgttgg tggcaccatg cagatgttgg
    24961 gtggtcgtca gggtggtggc gctcctgcag gtggcaatat cggtggtggt cagccgcagg
    25021 gcggttgggg tcagcctcag cagccgcagg gcggcaatca gttcagcggc ggcgcgcagt
    25081 ctcgcccgca gcagtctgct ccggcagcac cgtctaacga gccgccgatg gactttgatg
    25141 acgatattcc gttctgaaga tgaccttaga gttatctgta aagattgaaa acataggccc
    25201 ctgttaattc aggggttttt tgtttctatc gtcggaaaaa gtgtcttgta ttaattggtg
    25261 atttttcaca taaagtgtac cttgtaattg tgtaatgttt atagggtgtg aatgcatgga
    25321 aaagaactac agagttacat catttgtcat ggatagtggc gagcgctatt gcatggtagt
    25381 ggatcgtgct tctgggcttc ccatgtatta cccaactttg tttttaacaa cccaaatccg
    25441 caataaaggc aatgccttct caacgacgct agctgctgct aacaacctag tcttgttgtt
    25501 acgctttcta gatagtcgtg gcattaacct tgaacaacgg ttattgacca aagatttctt
    25561 caagtcacat gagatggacg atctgagaga ctttgctcag cgcaagcaag gcaggatgcc
    25621 atcaaaggtg ctctctaccc tttggttgga tgatgagttg accaatatag tggataacgg
    25681 aacacaacat tcccgcctaa caacctttgc caactattta cgctggtatg cgatgcatat
    25741 cttaaagatg cctgagctgg atgttgtcga acagatcaag actatggcga cgcaaataaa
    25801 ggtgcgccga ccgtctaaaa agagcagaaa tagtacattg caagacaggt ctttgagtga
    25861 cgttcaattg gatgtgctat ttgagacgat taagctcgaa tcagagttga atcctttttc
    25921 aattgatgtg cagcgccgca atcggctgat gatcttgctt ctgtatcatc tgggcatccg
    25981 aggtggtgaa ttattgaaca tcagaataca ggatatagat tttagctcta acaggatcct
    26041 tatcgttagg cgagcagatg aacgagctga ttctagaacc aatgagccta atgctaaaac
    26101 tagagaacgg ctcataccgt tggctgaatc cttagcccaa gaactgcaca gttttatcac
    26161 caaagatcgc cgcaaagtcc aaaatgcaaa gaaaaatgac tacctgtttg tgacttacaa
    26221 gcgtgggcca actgtcggaa aacctatctc caaggcggct tatcacaaaa tattttcctt
    26281 ggtcaaagat gtatcgccgc aattgtacgc aataactgga cactcgctcc gtcatacatg
    26341 gaatcgtaag ttttcagaaa gaatggatgc catggatggg ccggtcagcg aagaacgcca
    26401 agagcaaatc cgtgcgtact tacaaggttg gaaagcgggc tcaggaaccg cagcgactta
    26461 caacaagcgt ttcattcagc aaaaaggttt tgaggccgcc ttggcattac aagagggaaa
    26521 cggcactcgc ttgccaaagg gcataaagat tgatgatgaa taacatctta tcggtattgg
    26581 tttccgagct aatccctgca aaatcaaagg ggtactcttt catgttgtat gaaactatgt
    26641 ggagattaga taaaaacaac aatatttcgg ttggtatgat acatgacctt ctggatcctg
    26701 agactctaag tgggtttctt cataccctgt cattttatgc caggaacttg tccgcggcat
    26761 acacgagaca tattttccgg tgtttcttag acatgattcg tgcaactaaa gctactcgta
    26821 ttgacgatat catgttgatt ggatacaggt cttctttgga tgcggctcat gagcatgagt
    26881 taagctgcgt tagtatttta ctgcgaaagt ggtacaggct gggctatccc ggtgttagca
    26941 agaacgtggt gtgtttgctg gagggatgga ggttaaaggg ggggcgtaag ggggatgcgg
    27001 ttaagcgatt ggatcctcta gaggggccgt tgacggataa tgaacttcaa gcgtttaacg
    27061 aggcggttgt acgagcttat gaaaagagct taattaccat cgctgagctg gctatggctc
    27121 ttagcgtcag tcatacggga cgtcgttcaa ttcagatttc gcagctgcgc gtggttgata
    27181 ttctctgcgg agaaaatgaa aagggtgaac ccttctatgt gttgaatgtg ccgcgagcca
    27241 agcaacgcaa cagctttttt cgcgaaagct tcaagcccta tgcgatgact ctcgaactat
    27301 gggcagtcct gagtgctcag gcaaggaatg ctgttactct ggttgagaat catctcggga
    27361 ttgaattgca ggaagctgac cgactgcaga tccccctatt tcctgatctt gatgtgctag
    27421 cgaccatcca atccccttat gaattctgcc aactgcttac cactgacaaa ctccacatcg
    27481 ctgcttcagt gataactaaa acactccatt taatcgtaga agaatcagat atccggtctg
    27541 aacgtactgg tgatctattg catatcaatt ctcgccgctt tcggtatacc acgggaacac
    27601 gggctgcccg tgaaggtttt ggcgagttgg taatagcgga gctgcttgac catacggaca
    27661 cccaaaacgc aggtgtttat atcaagaata tacccgagca tgtgaaaatg ctggatgagg
    27721 cggtcggttt tcaattggcg ccatacgccc aggcatttgt cggggtactt gtagattcag
    27781 agagggatgc gcatcgaggt gacgacccaa ccagccgtat tcggaccgaa atgggtcagg
    27841 gtgttggctc ttgtggcgaa catggctttt gtggtgccaa tgttcctatt ccttgctaca
    27901 cctgcatgca ttttcagcca tggctgaatg gcccacataa cgaagtctat aagggcctgc
    27961 tcaatgagcg tgagtgggtg aaagagataa ctggcgacat ccagattgca gctgtgcttg
    28021 atcgtagcat cattgcagtg gcagacgtga tcatgcgttg tgctaaacgt cgtgaagagc
    28081 ttggtgaaga gggattaatt accaatggct gagattttcc agttcaagcc aaaggctatt
    28141 ctgaaggcag cagagaacct tgaagcattc atagttaagt gtcgagatga gttgacggta
    28201 ttcggctcta acctcaattg ggaagatcct gtttggccaa atataactgt gtttgccaag
    28261 ttgggcgtta ttactcgcaa accaaaacct caagaagtgc aagaccccgt attcatcgat
    28321 tttgccaaag cttatttccg ttaccagcag ggacatgcgc caacggggac taagaatgaa
    28381 agtaaagcct tgcgagcgat tgaggcgtct ttacttcaag taaacgggaa tgcaaatatt
    28441 gatggcctgt caattactgt gcttgatgaa gcagctgaat tggcaaggca gcactactca
    28501 gatggttcag catatagttg cggccgagag attgagcggc tggctaagtt tgtagttgaa
    28561 aaccagcttg tagaaaacgt tgtacaagat tgggtcaatc caattaagcg tgctgaagac
    28621 aagaacaaaa caggcaggga ggcgaagaaa aatagagaag agaaacttcc aagtgatatt
    28681 gctctcaatg cgttggcaga aatttttgcc aacgacccag ttcatgagcg agatatattt
    28741 acaacatcgg tatttgccat gttgatgtca gcaccgtcac gcattactga ggtgttatca
    28801 ttgcctgcag attgcgaggt attcgaaaag gatcgtgaag gtatagaacg ctacggctgg
    28861 cgtttttttg cagggaaagg ctatgagggt gatatcaaat ggataccaac cgtgatggtc
    28921 gacatcgcta aaatagccgt agctagagtt agagtgctct ctgaaaatgc ccgacagctg
    28981 gctaagtgga tagagaaaca cccggataaa ttttaccgcc atgctaactg ccctgatgtt
    29041 gctgatgatg tgccgttgac gatggttcag acttgtatgg cgctcggttt tgcacatcac
    29101 tctcaaaaaa cgtgcaggtc gtgtcttggt agccggggct tagctaagga agatggtgtg
    29161 catacgctaa atagcctgtg gcaacacact atgaaacgtt tgcccgaagg cttcccgtgg
    29221 tttgataaag acaaggggat taagtatagc aatgccttgt ttgtactgaa tgttaatcag
    29281 ttccacggaa accgaggatg tcttccggtt gaattacata agccaatcaa taactttttt
    29341 aacaatgatc tgactcctcg cgaggttctg tatggaaaac atgccactat ctttgatcgc
    29401 catggttatc gtactgataa tggagaacgg gtcaaattga cctcgcatca ggctcgacac
    29461 ctgctcaaca ccattgccca gcgtggagga ctgtcaaact tggaaattgc caagtggtct
    29521 ggacgggcag acgtgaagca gaatcgaacc tacaaccaca tgactgaata cgaactggtt
    29581 ggcatggctg aacgccttga tcccacaaaa gcgctatttg ggcctgttgg cgaagtggca
    29641 aagcatctgc ccgtgactat gcaggagttc aataccttag agcatgccgc agtccatgtg
    29701 acggagtatg gctactgtgt gcatgattac accatgagtc cttgtgagaa gttccgagat
    29761 tgcgtcaatt gtaccgaaca ggtttgcatc aaaggcgatg atactgaaaa gttggatcgc
    29821 attaagaaac gtctggagaa gtcggagcga ctattttttc ttgctgacgc agcggtagat
    29881 agtggcgaga taggaattga tcgctggtat cagtatcaca agaaaactgt tacccggctt
    29941 tgtgagctag tggatatttt agagaatccg gacattgaaa atggtgctca gattaagctc
    30001 cggggcaatg atttcagcca gcttcgccgt gttgtcgcaa agacgtctat tgaggccatc
    30061 gagcaaaaag gcaaagaaag tgaagaagct gtgatgttag atgatttgac aactttgtta
    30121 gggggtggcc ttggctaagc atctggacaa tagagacatt aacgccatcg tgaatctaat
    30181 tcgaggatgg caggaaccaa aactgtcctg gtcagcaatc tgtgaggcgg cagaaccatt
    30241 ggtcggaaag ttgccgtcaa gacagtctct taatgcacat gaggcaatag tgactgccta
    30301 tcaaacaaag aaagaggcgc ttaaaggccg tggccccaaa aatccgcgtc cagccagttt
    30361 gaacattgca gccgctagga ttgtgaatct tgaaagcgaa atcgaagagc ttaaggaaga
    30421 gaaccgtatg tacaaacaac agtttgtgat ctggcaatac aatgcataca aacacggaat
    30481 gaaggaacat caacttaatg cgcagttgac aaagattgac cgggagcgtt ctgacggtga
    30541 aaaacgataa taatctcggt agtataattt ttcgtaatat tatttataaa ggagattgaa
    30601 aatgattgac cagtcttttt cagcgaggaa ttttagaagg atatacgacc tggataggaa
    30661 aaataaaggt actttagaag ttgaacactt tcctgatgct tataagatcg ggaagaaaat
    30721 aacaatgtta aaaggtttta tttattggct cgcaagaaga ctgagaagtg gtcagattag
    30781 cacggctaaa tttgaagaaa agaaagataa aattaatgag catattaagc ttagaaaaga
    30841 acaatataat aatgaagtga atagcaagtt gaaaattatt gcacagaatg tcggtataaa
    30901 agggtacgcg ttacctttga cgttattgcc acatcaagtg aagaataaaa atgtatattc
    30961 aattggaaat tcagttgaag cattatttgt gtctaggcaa ataaaatata ttttaagttc
    31021 gttatacaca ataaaatcaa ataataggga tctgattatc agtcgcctaa gcgccttggc
    31081 taaggaaatg agtccaaagt atataatcag ggccgatgtt gaaagttttt atgaatctat
    31141 aaaccataag tctttattag atatgttgca ttcatcacct aaattatctg ttccgccacg
    31201 tcgggtgatt actcagttaa ttaggaaata tcagacttta actgggctag ataaaggggt
    31261 acctcgtggt gtaggcataa gttcttattt atctgagctg tatatgagtg ttgtagatga
    31321 taaaataagg gccttaccag atgttactta ttatgaacgg tttgtagatg atttgatagt
    31381 gatcttttca ccagataaag gggggaatac ttgtagctat ctcccccaaa ttactagcat
    31441 tataaatgac cgtgccttgt gccttaataa taaaacaaaa gaattggatt tatttacaca
    31501 atctaataaa agctttgaat atctaggcta caaatttcag ctaacagcta gagactgtag
    31561 tataaagatg agttcaaata aaattcaaaa aattagaagt cgaattgata atacatttga
    31621 tgaatataat cagagtgtta gtaaaacacc aaagagagca gcaaaaaata ttttgcttag
    31681 aatgaaattt cttaccggaa acactcgtct ttataatagt aaatcaaaag cctttgttgg
    31741 gatatatttt agcaatcgat tcataactga tttatctgat ttaaatggat tggattctta
    31801 tttggcgaat aaaattaatg gtttgcaaga tcagaaactt aaaaagagaa
//
LOCUS       DAPOZO010000057        31749 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPOZO010000057 DAPOZO010000000
VERSION     DAPOZO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.057"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(53..1420)
                     /locus_tag="ROI21_002801"
     CDS             complement(53..1420)
                     /locus_tag="ROI21_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDX5111702.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVVG
                     GPIVAHILSSHYESYNLHIAELTNENDQVVWKTSYVTIMIFMWLTLLSVNLYFNGLRC
                     DIWNGVTVIAFCAVLYNVSLLFMSRYSVSIWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKDIAHRDSLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVSIIGKSIRPDDLFARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVKEAEILIN
                     RKDDE"
     gene            2012..4435
                     /gene="pgaA"
                     /locus_tag="ROI21_002802"
     CDS             2012..4435
                     /gene="pgaA"
                     /locus_tag="ROI21_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDX5111703.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAANNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLESQNKDYQRGQILTLADAGHYDSALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHEDELRAMTGSLPENDLTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWAQYWVASAYLKEHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIIRDWLAGVEWRSRNIWLEAEYTERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            4444..6462
                     /gene="pgaB"
                     /locus_tag="ROI21_002803"
     CDS             4444..6462
                     /gene="pgaB"
                     /locus_tag="ROI21_002803"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDX5111704.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHQGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            6455..7780
                     /gene="pgaC"
                     /locus_tag="ROI21_002804"
     CDS             6455..7780
                     /gene="pgaC"
                     /locus_tag="ROI21_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDX5111705.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWAIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            7782..8195
                     /gene="pgaD"
                     /locus_tag="ROI21_002805"
     CDS             7782..8195
                     /gene="pgaD"
                     /locus_tag="ROI21_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDX5111706.1"
                     /translation="MNNLIITTRQSPVRLMVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTRQGQIKMVVSEKALVRA"
     gene            complement(8245..9033)
                     /gene="phoH"
                     /locus_tag="ROI21_002806"
     CDS             complement(8245..9033)
                     /gene="phoH"
                     /locus_tag="ROI21_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDX5111707.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(9653..10924)
                     /gene="efeB"
                     /locus_tag="ROI21_002807"
     CDS             complement(9653..10924)
                     /gene="efeB"
                     /locus_tag="ROI21_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDX5111708.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIASDNLTITLSVGHSLFDERFGLTPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQQVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(10930..12057)
                     /gene="efeO"
                     /locus_tag="ROI21_002808"
     CDS             complement(10930..12057)
                     /gene="efeO"
                     /locus_tag="ROI21_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDX5111709.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(12115..12945)
                     /locus_tag="ROI21_002809"
     CDS             complement(12115..12945)
                     /locus_tag="ROI21_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDX5111710.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(13599..15107)
                     /gene="putP"
                     /locus_tag="ROI21_002810"
     CDS             complement(13599..15107)
                     /gene="putP"
                     /locus_tag="ROI21_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDX5111711.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(15266..15475)
                     /locus_tag="ROI21_002811"
     CDS             complement(15266..15475)
                     /locus_tag="ROI21_002811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111712.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            15530..19492
                     /gene="putA"
                     /locus_tag="ROI21_002812"
     CDS             15530..19492
                     /gene="putA"
                     /locus_tag="ROI21_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDX5111713.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPIAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGYVREATPS
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDVQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(19532..20170)
                     /gene="rutR"
                     /locus_tag="ROI21_002813"
     CDS             complement(19532..20170)
                     /gene="rutR"
                     /locus_tag="ROI21_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDX5111714.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            20458..21549
                     /gene="rutA"
                     /locus_tag="ROI21_002814"
     CDS             20458..21549
                     /gene="rutA"
                     /locus_tag="ROI21_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDX5111715.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     NFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARTKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPSAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            21549..22241
                     /gene="rutB"
                     /locus_tag="ROI21_002815"
     CDS             21549..22241
                     /gene="rutB"
                     /locus_tag="ROI21_002815"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDX5111716.1"
                     /translation="MTILTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDAQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            22253..22639
                     /gene="rutC"
                     /locus_tag="ROI21_002816"
     CDS             22253..22639
                     /gene="rutC"
                     /locus_tag="ROI21_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDX5111717.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            22647..23459
                     /gene="rutD"
                     /locus_tag="ROI21_002817"
     CDS             22647..23459
                     /gene="rutD"
                     /locus_tag="ROI21_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDX5111718.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQIVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVREKLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSRHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAACKELL"
     gene            23456..24046
                     /locus_tag="ROI21_002818"
     CDS             23456..24046
                     /locus_tag="ROI21_002818"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDX5111719.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFT
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            24057..24551
                     /gene="rutF"
                     /locus_tag="ROI21_002819"
     CDS             24057..24551
                     /gene="rutF"
                     /locus_tag="ROI21_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDX5111720.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            24572..25900
                     /gene="rutG"
                     /locus_tag="ROI21_002820"
     CDS             24572..25900
                     /gene="rutG"
                     /locus_tag="ROI21_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDX5111721.1"
                     /translation="MAMFGFSHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMDVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(25983..26156)
                     /locus_tag="ROI21_002821"
     CDS             complement(25983..26156)
                     /locus_tag="ROI21_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDX5111722.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            26529..27125
                     /gene="wrbA"
                     /locus_tag="ROI21_002822"
     CDS             26529..27125
                     /gene="wrbA"
                     /locus_tag="ROI21_002822"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDX5111723.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            27146..27373
                     /locus_tag="ROI21_002823"
     CDS             27146..27373
                     /locus_tag="ROI21_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDX5111724.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(27411..28652)
                     /gene="agp"
                     /locus_tag="ROI21_002824"
     CDS             complement(27411..28652)
                     /gene="agp"
                     /locus_tag="ROI21_002824"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDX5111725.1"
                     /translation="MNKTLIAAAVAGIFLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSSCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(28945..30201)
                     /locus_tag="ROI21_002825"
     CDS             complement(28945..30201)
                     /locus_tag="ROI21_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HDX5111726.1"
                     /translation="MGSNIHGISCTANNYLKQAWHNIKKEHEKNQTYSITLFDNTLVC
                     FMRLYKEIRRQKAEDYIPCLECESLEKEFEEMQNDNDLSLFLRTLRTNDTKIYSGVSG
                     GMTYTIQYIQDIDVVRVSLPGRASESITDFKGYYWYGFMEYIENINACDDVFSEYCLE
                     DENISVQPEQINTPGISDLDTGIDLSGISFIQSEINKTYELKYAPVDGDGYCLLRAIL
                     VLKQHDYSWALGSHKTQKEVYEEFIKIVDKPTIEALVDAAFCDLREDVKTLFGVDLQS
                     DNKIQGVGSLMSWSFLFFKKEFIDSCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFAQAYKLNNKEMNAQLDYLNRYAI"
     gene            30461..31381
                     /gene="cbpA"
                     /locus_tag="ROI21_002826"
     CDS             30461..31381
                     /gene="cbpA"
                     /locus_tag="ROI21_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDX5111727.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            31381..31686
                     /gene="cbpM"
                     /locus_tag="ROI21_002827"
     CDS             31381..31686
                     /gene="cbpM"
                     /locus_tag="ROI21_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDX5111728.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
BASE COUNT         8023 a         8050 c         7780 g         7896 t
ORIGIN      
        1 tagaccgggg ggatgagaag ccccaggcgg aggtcgacaa ctagcgacag actcattcat
       61 catccttgcg gttgataaga atttctgcct cttttactac caccttatta cgcccggtct
      121 ctttcgcctc gtatagtgcg ttatcagcca gtcgatagat ctcattcggg tttaacgcat
      181 tttcctgagt cacaacagcg ccaatactaa tcgtcacttt ttgtggtatg gcgtattcag
      241 gattatcacc agttaaacgc tcaacatttt cccgaatcct ttccgctaaa gctttcgcac
      301 gttcagtatc gatgtccgtc agcaagacgc caaactcttc gccgccgacg cgcgcaaaaa
      361 ggtcatctgg ccgtatgctt ttcccaatga tactcaccac tgtttttatc acctgatcgc
      421 caaccggatg cccccaggtg tcgttgactt ttttgaagtg gtcgatatcc ataatcatga
      481 cgcaataagg cgtttttttg gccgatgctg attgaactgt cagttcatta aaaaaataat
      541 ttctgttaaa tatattggtt aaggaatcgc gatgtgcaat gtcctttgtt actcgtagcg
      601 cactgaatat atggcacata aatattacca taacggttaa cttactgact acttcaatag
      661 tgcgactaat ataccaaatg gacacgctat acctgctcat aaataaaaga gagacattgt
      721 acagtacggc acaaaaagca atcaccgtca ctccattcca gatatcacag cgtaaaccat
      781 taaagtatag attaactgaa agcaaggtta accacatgaa aatcattatg gtaacatatg
      841 atgttttcca aaccacctga tcattttcat tggttaactc ggcaatgtgt aaattgtagc
      901 tctcataatg actagaaagt atgtgagcaa ctatcggccc cccaacaaca aaaaacaaac
      961 ttatgcacaa agtcattttt ttggaaaata aattgcgctg ttttgtgttt tcactaactt
     1021 tattcaccaa tgccagaaat attaatatgc acaaactcat ctgacgaaac aaataataga
     1081 ttgcaatgtc attttgaaca acactgcttg tcaaacgccc ctcaataatt tgctgaatga
     1141 taatgacggt tttaacaaag taaatcaaac aactcagaaa agccaaacta agtatacagt
     1201 ttgaaaattg cctacggtca taaatgaaat acaactgcat aaaaataaat gcaacagtat
     1261 cgataaaaaa taaagcaatc attaccatca gataggttga tcgtggaaca acttcctcaa
     1321 cgcctggctg attaaaccaa ctattcacca aaaccaaagc aagcccaaac aataagctca
     1381 ctaatacagt actactaatt ctcaaatagt ctttttccat aaagctacac caatctgtag
     1441 gttgtagatc cctttctatt attaatagat ataagaactg tactgttgtc aatatgaaat
     1501 tccgcccaca aacaataccg atgatgatag ttgcagactg caattattat taccttagtg
     1561 ctttatacat tcttcgcgac aagacaggct tgcaaataca tataaatggt tataaggaaa
     1621 tccttataaa acattaattc atagatatat taggaataac aattgaatcc gtgagtacag
     1681 taacgctgca tttcatcagg actttcgaaa aatccgatat catgcatcgg aatttactga
     1741 tttaattatt ttaatcctaa tttattttga aaaaggcatt gggatttatg ccgtattcct
     1801 gaagttcctc atcattggaa tggattttcg ggcgagaaaa ggattttata tggatactct
     1861 actcatcatt tcttcttctc atcatcaaca attcacgtct ctcttccgcg tttaataacg
     1921 gattatgatg tgcaaaaaaa aatatctttc gtttcagtta cctgtaatta gatacagaga
     1981 gagattttag caatacatgg agtaatacag gatgtattca agtagcagaa aaaggtgccc
     2041 gaaaaccaaa tgggctttga aaattcttac tgccgcattt ttagcagcga gtcccgcggc
     2101 gaagagtgct gctaataatg cctatgatgc attgattatc gaagctcgca agggaaatac
     2161 tcagccagct ttgttatggt ttgcacaaaa atcagcactc agtaataacc aaattgctga
     2221 ctggttacag attgccttat gggccgggca agataaacag gttattaccg tttacaaccg
     2281 ctaccgtcat cagcaattac cagcgcgtgg ttatgcagct gtcgccgtcg cttatcgtaa
     2341 cctgcaacaa tggcaaaact cgcttacact gtggcaaaag gcactctctc tggagtcgca
     2401 aaataaggat tatcaacggg gacaaatttt aaccctggca gatgcgggtc actatgattc
     2461 tgcgctggtt aaacttaagc agcttaactc tggagcaccg gacaaagcta atttactcgc
     2521 agaagcctat atctataaac tggcggggcg gcatgaggat gaattacggg cgatgacagg
     2581 gtcattacct gaaaatgact taacgcaaca atatcccaca gaatacgtgc aggcattacg
     2641 gaataatcaa cttgctgccg cgattgacga tgccaattta acgccagata ttcgcgctga
     2701 tattcatgcc gaactggtca gactgtcatt tatgcctacg cgcagtgaaa gtgaacgtta
     2761 tgccattgcc gatcgggccc tcgcccaata cgctgcatta gaaattctgt ggcatgataa
     2821 ccctgaccgc actgcccagt accagcgtat tcaggttgat catcttggcg cgttattaac
     2881 tcgcgatcgt tataaagacg ttatttctca ctatcagcgt ttaaaaaaga cggggcaaat
     2941 tattccgccc tgggcgcaat attgggttgc gtcggcttat ctcaaagaac atcagccaaa
     3001 aaaagcacag tcaataatga cagagctctt ttatcacaag gagaccattg ccccggattt
     3061 atccgatgaa gaacttgcgg atctctttta cagccacctg gagagtgaaa attatcccgg
     3121 cgcgctaact gtcacccaac ataccattaa tacttcgccg cctttccttc ggttaatggg
     3181 cacgcctacg agcatcccga atgatacctg gttacagggg cattcgtttc tctcaaccgt
     3241 agcaaaatat agtaatgatc ttcctcaggc tgaaatgaca gccagagagc ttgcttataa
     3301 cgcaccagga aatcagggac tgcgcattga ttacgcgagt gtgttacaag cccgcggttg
     3361 gcctcgtgcg gcagaaaatg aattaaaaaa agcagaagtg atcgagccac gtaatattaa
     3421 tctggaggtt gaacaagcct ggacagcatt aacgttacaa gaatggcagc aggcagcggt
     3481 cttaacgcac gatgttgtcg aacgtgaacc gcaagatccc ggcgttgtac gtttaaaacg
     3541 tgcggttgat gtacataatc ttgcagagct tcgtatcgct ggctcaacag gaattgatgc
     3601 cgaaggtccg gatagtggca aacatgatgt cgacttaacc accatcgttt attcaccacc
     3661 gctgaatgat aactggcgcg gttttgctgg attcggttat gccgatggac aatttagcga
     3721 aggaaaaggg attattcgcg actggcttgc gggtgttgag tggcggtcac gtaatatctg
     3781 gcttgaggcg gagtacaccg aacgcttttt caatcatgag cataaacccg gcgcgcgcct
     3841 gtctggctgg tatgatttta atgataactg gcgtattggt tcgcaactgg aacgcctctc
     3901 tcaccgcgtt ccattacggg caatgaaaaa tggtgttaca ggcaacagtg cacaggctta
     3961 tgttcgctgg tatcaaaatg agcggcgtaa gtacggtgtc tcctgggctt tcactgattt
     4021 ttctgacagt aatcagcgtc atgaagtctc acttgagggt caggaacgca tctggtcttc
     4081 accatatttg attgtcgatt tcctacccaa tctgtattac gaacaaaata cagaacacga
     4141 cactccatac tacaacccta taaaaacgtt cgatattgtt ccggcatttg aggcaagtca
     4201 tttgttatgg cgtagctatg aaaatagctg ggagcaaatc ttcagcgcag gtgttggtgc
     4261 ctcctggcaa aaacattatg gcacggatgt cgtcacccaa ctcggctacg ggcaacgcat
     4321 tagctggaat gacgtgattg atgctggcgc aacgctacgc tgggaaaaac gaccttatga
     4381 cggtgacaga gaacacaact tatacgttga attcgatatg acattcagat tttaaggata
     4441 aatatgttac gtaatggaaa taaatatctc ctgatgctgg tgagtataat tatgctcact
     4501 gcgtgcatta gccagtcaag aacatcattt ataccgccac aggatcgcga atctttactc
     4561 gccgagcaac cgtggccgca taatggtttt gtggcgattt catggcataa cgttgaagac
     4621 gaagctgccg accagcgttt tatgtcagtg cggacatcag cactgcgtga acaatttgcc
     4681 tggctgcgcg agaacggtta tcaaccggtc agtattgctc aaattcgtga agcacatcaa
     4741 ggaggaaagc cgctaccgga aaaagcagta gtgctaactt ttgatgacgg ctaccagagt
     4801 ttttataccc gcgtcttccc aattcttcag gccttccagt ggcctgctgt atgggccccc
     4861 gtcggcagtt gggtcgatac gccagcggat gaacaagtaa aatttggcga tgagatggtc
     4921 gatcgagaat attttgccac gtggcaacaa gtgcgagaag ttgcgcgttc ccggctcgtt
     4981 gaggtcgctt ctcatacatg gaattctcac tacggtattc aggctaatgc caccggcagc
     5041 ttattgcctg tatatgtaaa tcgtgcatat tttactgacc acgcacggta tgaaaccgca
     5101 gcagaatacc gggaaagaat tcgtctggat gctgtaaaaa tgacggaata cctgcgtaca
     5161 aaggctaagg taaatccaca cgtttttgtt tggccttatg gcgaagcgaa tggcatagcg
     5221 atagaggaat taaaaaaact cggttatgac atgttcttca cccttgaatc aggtttggca
     5281 aatgcgtcac aattggattc cattccgcga gtattaatcg ccaataatcc ctcattaaaa
     5341 gagtttgccc agcaaattat taccgtacag gaaaaatcac cacaacggat aatgcatatc
     5401 gatcttgatt acgtttatga cgaaaaccgc cagcaaatgg atcgcaatat tgatgtgcta
     5461 attcagcggg tgaaagatat gcaaatatca accgtgtatt tgcaggcatt tgctgatccc
     5521 gatggtgatg ggctggtcaa agaggtctgg tttccaaatc gtttgctacc aatgaaagca
     5581 gatattttta gtcgggttgc ctggcaatta cgtacccgct caggtgtaaa catctatgcg
     5641 tggatgccgg tattaagctg ggatttagat cccacattaa cgcgagtaaa atacttaccg
     5701 actggggaga aaaaagcaca aattcatcct gaacaatatc gccgtctctc tcctttcgat
     5761 gacagagtca gagcacaagt tggcatgtta tatgaagatc ttgccggaca tgctgctttt
     5821 gatggcatat tgttccacga tgatgctttg ctttcagatt atgaagatgc cagtgcaccg
     5881 gctatcacgg cttatcagca agcaggcttt agcgggagtc tgagcgaaat tcgacaaaac
     5941 ccggagcaat ttaaacagtg gacccgcttt aaaagtcgtg cgttaactga cttcacttta
     6001 gaacttagtg cgcgcgtaaa agccattcgc ggtccacatg ttaaaactgc acgaaatatt
     6061 tttgcacttc cggtaataca acctgaaagt gaagcctggt ttgcacagaa ttatgctgat
     6121 ttcctaaaaa gctatgactg gaccgctatt atggctatgc cttatatgga aggtgtcgca
     6181 gaaaaatcgg ctgaccaatg gttaatacaa ttgaccaatc aaattaaaaa catccctcag
     6241 gctaaagaca aatctatttt agaattacag gcacaaaact ggcagaaaaa tggtcagcat
     6301 caggctattt cttcgcaaca actcgctcac tggatgagcc tattacaact gaatggagtg
     6361 aaaaactatg gttattatcc cgacaatttt ctgcataacc aacctgaaat agacgttatt
     6421 cgtcctgagt tttcaacagc ctggtatccg aaaaatgatt aatcgcatcg tatcgttttt
     6481 tatattatgt ctggtgttat gcatacccct atgcgtagcg tactttcact ctggtgaact
     6541 gatgatgagg ttcgttttct tctggccgtt ttttatgtcc atcatgtgga ttgttggcgg
     6601 cgtctatttc tgggtctatc gtgaacgcca ctggccgtgg ggagaaaacg caccagctcc
     6661 ccagttgaaa gataatccgt ctatctccat tatcattccc tgttttaatg aggagaaaaa
     6721 cgttgaggaa accatacacg ccgctttagc acagcgttat gagaacattg aagttattgc
     6781 cgtaaatgac ggttcaacag acaaaacccg tgccatcctg gatcgcatgg ctgcacaaat
     6841 tccccacctg cgggtcattc atctggcgca aaaccagggg aaagccattg cgcttaaaac
     6901 cggagctgcc gcggcgaaaa gtgaatatct ggtgtgcatt gatggcgatg cgttattaga
     6961 ccgcgatgcg gcggcatata ttgtggaacc gatgttgtac aacccgcgtg tgggtgccgt
     7021 aaccggtaat cctcgtattc gaacacgttc taccctggtg ggtaaaattc aggttggcga
     7081 gtattcctca attattggtt tgatcaagcg aacccagcgt atctatggaa acgtatttac
     7141 cgtttccggt gttattgccg catttcgtcg cagcgccctg gcagaagtgg gttactggag
     7201 tgacgatatg atcaccgaag atattgatat tagctggaag ctgcagttga atcagtgggc
     7261 gattttttac gagccacggg cgctgtgctg gatattaatg cctgaaacgt taaaagggct
     7321 gtggaaacag cgcctgcgct gggctcaggg cggtgcagaa gtattcctca aaaatatgac
     7381 aaggttgtgg cgcaaagaaa actttcgaat gtggccgctg ttttttgaat actgcctgac
     7441 gacaatatgg gccttcactt gcctggtcgg tttcattatt tacgcagtcc aacttgccgg
     7501 tgtaccgtta aatattgaat tgacacatat cgctgcgaca catactgccg gaatattatt
     7561 gtgtacgtta tgtttactgc aatttattgt cagcctgatg atcgagaatc gctatgagca
     7621 taatctgact tcatcgcttt tctggattat ttggttcccg gttattttct ggatgctgag
     7681 tctggcaacg acattggtat catttacacg agtcatgttg atgcctaaaa agcaacgcgc
     7741 ccgttgggta agtcccgatc gcgggattct gagaggttaa tatgaacaat ttaattatta
     7801 cgacccgaca atcgccagta cgtttaatgg ttgattatgt tgccacaacc atcttatgga
     7861 cattatttgc tttgttcata ttcttattcg ccatggatct gctgacgggt tattactggc
     7921 aaagcgaggc cagaagccga cttcagttct attttttgct ggcagtggcg aatgccgtcg
     7981 tgttaattgt ctgggcgctg tacaataagc tacgttttca aaaacagcag catcatgcag
     8041 cctaccaata tacgccgcaa gaatatgctg agagcttagc aatacctgat gagctctatc
     8101 agcaactaca aaaaagccac aggatgagcg tacacttcac caggcagggg caaataaaaa
     8161 tggtggtttc agaaaaagcg ctagtccggg cgtaaacacc caaaacaaag cccggttcgc
     8221 ccgggctctg cgccgataat acacttaact gtatgcatgt agcgtacgtt ggcaaagtgc
     8281 cgaacgtacg caatcctctt taccgaatcg gacgatcccg accatttcat cttcttcgaa
     8341 acgttccagc gcgtcactta atccggagcg cacgccgcga ggcaaatcgc attgcgtgat
     8401 atcaccgttg acgataaccg tcacgttctc cccgaggcgg gttaaaaaca ttttcatttg
     8461 cgcggcagtc acattctgcg cctcgtcaag aatgacgact gcattttcaa aggtacgtcc
     8521 acgcatatag gcgaacggcg caatttccac cttccctatt tccggtcgca ggcagtactg
     8581 cataaaggaa gcccctaagc gccggaccag cacgtcgtag accggacgaa aatagggagc
     8641 aaacttttct gcgatatctc caggtaagaa gccaagatct tcatcggctt gcagaactgg
     8701 acgggtgacg ataatcctgt cgacatcctt atgtatcagg gcctctgccg cttttgctgc
     8761 gctgatccag gtttttccgc acccggcttc gcccgtggcg aatatcagct gcttactctc
     8821 aatagccttc agatagtgca attgcgcttc atttcgcgcg aggatgggcg aggtatcgcg
     8881 actgtcgcgg gccataccaa tggcttctac gccgctcatc tgcacaagcg aggtgaccga
     8941 ttcttcttca cgctgcttat ggctgcgcga atcccgtctc agcacacgtt ttgcctcgcg
     9001 acgagctttg atcactgctt tttgtcttcc catggagagc accttgagtt gtttgtattc
     9061 atcacacgcg ccgttggcag cgcgattatg cgcacgaaca tcagagggtt ggcttccttg
     9121 taagccatag tttgcttttg gataaaatgc cgaaaaacgg ctacgcacac cgtttgcggc
     9181 gtcggtaaca cgagtaaaga gaggaagtga gaaaaataca gagtgacgaa gttgctaccg
     9241 gaggagaaat ctccgcgagt ggtggagcct tgcttaacaa aacattgtcc agaacaggac
     9301 gttaccatcc gcgatctcca tagtgactga ctatcactgc cgggaacttc cgctgctact
     9361 taataagtac aacagatctc gcattcattg caacaatata ttcacttata tttaactaca
     9421 aaacaccatt tcagtgacat tagtttctac tggaaagatg acagagtgat gacagtgatg
     9481 aaaaaagctg tgtgctttca gcaggattcg catcaggcat cagcgcacga acgccggatg
     9541 cggcgtgaac gcctgatcca gcctaccaaa cccgcaccga ttgtaggcat gataagacgc
     9601 gcaagcgtcg catcaggcat ctgcgcacga acgccttatc cgcctaaaca cattaaaccc
     9661 gcaataacgc gcttccgaga taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat
     9721 aaccgccgcc gataggttta acgtattcct ccagcgcttc gccattgagc cttttttgta
     9781 ctgtcaggaa gcctttttcc agatcgtgtt gatagcagac aaacagtagc cccatatcca
     9841 gttgtccgga gttggtgacg cccaacgaat aactgtagcc acgacgcagc atcaggctgg
     9901 actcactctc tggcgtgcgg ggattcgcca gccggatatg gctgtccagc gcaatcacct
     9961 tcccctccgg atcgctggcg taatcgggaa catcatgctc atgctgcatt cccagcggcg
    10021 caccggtttg tttgtcgcga ccaaaaatcg tctgctgttc tttcagtggc gttctgtccc
    10081 aaaactccac ccgaaactga atcaaccgca ccgcctggta gctgccgccg attgtccacg
    10141 caggctcctg ctgatctgcc gtcacccaca ccacttgttg catcaactta tcattctggc
    10201 tatcgggatt ggcagtgccg tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt
    10261 tactacgcgc tgcatgatcg gaaataaacc cttcccgctt ccagcgcacg ctgagcaaat
    10321 ccggcgtgtg tttgatgata tcgcgcagcg catggatcac cgtgtcctgg gtgttggcgc
    10381 aaatttgtag caacacatca ccatgacata acgccgcatc cagcgagtcg ttggggaaac
    10441 gcgtcatttt ctgcaacttt ttcggcatct gcggtgtaag gccaaaccgc tcatcaaaca
    10501 atgagtggcc caccgataac gttatggtga gattatcgga cgcaatgtag ccgccaagaa
    10561 tgccggaatc gagtggaggc aggcgcggat ttggcgtttc tggtgccgct ccgccctgag
    10621 tcagaaaagc aaaacgctga gtcaacaagc gaaacaacct ttcaagatcg gctttatcgc
    10681 tggcaagcac atcaaacgcc accagcatca ttgcggcctg ttgtggcgtc aggatccctg
    10741 cctgatgctc accataaaac ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg
    10801 gtgcactttg cgttttttgt gcatgagcga ccggacaact tcccgccagc gccagcgcac
    10861 caatcccttt cagtaaacgt cggcgtgacg gttcattcac gccgttttca tcttcatact
    10921 gcataacgtt taatccagcc ccagcacacc gcgaagttgc gccagatctt ccgccagcgc
    10981 agtaatcggc cctttcagcg cattacggtc agcatcggtc aatttgtcgt aggtttcaaa
    11041 accgtcttta gtacggtatt tcgccagaat ggtatcgacc tttttgaaat tggcatcgac
    11101 ttttgccagc agttccgggt tggccttttg cagttgtgga cgcagcagat cgacaatttt
    11161 ctgcgagcct tcaacgttag cctggaaatc ccacagatcg gtgtggctgt agcgatcttc
    11221 ttcaccgctg attttgctgg ctgccacttc ctcaatcagt ccggctgcac cgccgaccac
    11281 ttttgaaggt gggaaagcca gttcactgat gcgtttttgc aaatcgacca catcggtata
    11341 aagctggtcg gcatacttat ccatcccttt agtggtgtta tcgccaaaca atgctttttc
    11401 cagacggtgg aaaccggtga attttggatc ggcggctttt tgctcgtaat catcttcacg
    11461 ggcgtcaatg ctaccatcca gatcggagaa cagttcagca atcggttcaa tgcgctcata
    11521 gtgctggcgc gtcggcgcat acagtgcttt tgctttttcg atatcgcctg ctttaatagc
    11581 gtcggtaaag gctttggtgt cggtcaccag ctgcgtggtt tccgccatga catacgcttt
    11641 atatgcagta attgcaccac caagacttaa cagcgcatca ctttgcgccg catccgccgt
    11701 tgcctcacct ttgacgatca acttcccttt cgggttagtc agcagaccgc aggtcatatc
    11761 gtattcgcca ggctgtaaat tcgccgtcat tttctggcta aaaccagggg cgatattttc
    11821 ccgctcttcc accaccatca cgcctttgag gatctcccac tccagcgcct tctggctgtg
    11881 gttctgaata atgaactgtg ttttcccggc gttaaccgta atggtcatcg gttcgcactg
    11941 cttatccgtc acggtcactt tgacctgcgg cacatcagcg gcgttagcca taaaagcaga
    12001 agaaaacagc gcagccacgc tcaactgcaa tgcgttacgg cggaagttaa tggtcatgac
    12061 gagactatcc ctttaaagta tgttgtaact aagcaagagt ttgcgtcgta tttgctacgc
    12121 agagcgagac gctgtcgccc ctgcgcgtgg tggcagagca aatgccacca gcgccgggat
    12181 gagataaata aaccagacgg cgacttcgct gacacttggc gcttcctgat agccaaaaat
    12241 cccttccatc agcgtgccaa acagcgagtg agttgagagc accgcactca tatcgaaggc
    12301 gatttcctga aagtggttcc acaatccggc ttcatgaaat gcgcgaatgg caccagccgc
    12361 cagccctgcg gcgacgaaga gaataaacag gctggtccat ttaaaaaatg caccaagatt
    12421 gaggcgaata ccgccccagt agagcaggaa gccgagcacc acggctgtag caagaccgag
    12481 cattgcaccc agcggcggcc agatcccgac atcttgttga aacgccgcca gcaggaaaaa
    12541 gaccgactcc agcccctccc ttgcaacggc aaaaaagacc atcatcacca gcgcccagcc
    12601 atgatgattt ccacgctgca atgcgctatc gactgcctgc tccagttgta ctttgacgtt
    12661 gcgcgacact ttgcgcatcc agaaaaccat ccaggtaagg atcaccacgg cgatcaccgc
    12721 cacgatacct tcaaacagtt cctgttcttt ttgcggaaat tcgccggtgg tttcgttaat
    12781 gaagataccc aagcccaggc acaacgcagc ggcaagcaac acgccaatcc acatcacacc
    12841 aatccatcgg cctcgctggg tacgcttaag atagctggcg atcaaactga caatcagcgc
    12901 ggcttcaagt ccttcgcgca acataatgag aaatggaaca aacatgccac ccacccttaa
    12961 acgtttttct gagtcaatct atgcaaagaa aagtaaatcg cagtgatagc gattatcatt
    13021 acggcaaaga aaaatacaag cggaatgttt ataaaatgat gttggaatgt aaacaaagag
    13081 aggtaatgcc aggggatata aacgattatt ccaggtacaa agatgggatt gtttacaacg
    13141 agccatgtgt tcttgccaga tgccgcgtaa acgccttatc cagccgacaa aactgtccag
    13201 actcaatgca tcagagcttc catgtaaaac tgataagcgg gtgtatcaag caatttcgtc
    13261 catccgcgat tatcaaatct aactttttgc ccggaacata ataacagctc gggtgttggt
    13321 ggcgtcaaag tcgctgaagc gttccccgaa ggccggggca gcccacatgg atgtgggctg
    13381 agggcgcgtc ttacaggaca ttacctcgcg cccgacccgg tagccgaagg ggataagctg
    13441 aaggtaccgc gcagcggcga ttttgttcgc cagagcccgg ggtgcagggg gtggcggcga
    13501 ttggccgccc cctgcaccat tcaatttata tggcgaggca cattaaggga gcgcaacccg
    13561 ctgctgaagc caaacaaaac cgccgcaggc taagtccctt agctttcctg caaccgtgac
    13621 ggcggagccg aatgatagtg cgcatcggcc tcggcaaagc gtttttgcat cgccgctgac
    13681 ggcgctttac ccagcaaact aaacactaca atcccgatac taccaaagat aaagcccgga
    13741 ataatttcgt acagacccag ccagccgaac tgtttccaga cgataaccgt cagcgcaccg
    13801 atgatcatcc ccgccagcgc accgttgcgc gtcatgcgtg accacatcac cgagaacagc
    13861 accaccggac caaacgccgc gccaaagcct gcccacgcgt agctcactaa gcccagcacg
    13921 cggttttccg ggtttgccgc cagcgcaatg gccaccagcg ccaccaccag caccatcaca
    13981 cgccctaccc acaccagctc tttctggctg gcctgtttac gcagaaacgc tttgtataaa
    14041 tcttcggtaa tcgcactgga gcacaccagc agctggcaac ttaaggttga cattaccgcc
    14101 gccagaatcg ccgacagcag aatcccggca atccacgggt taaacagaat ttgcgccagt
    14161 tcgataaaca cacgttcagc attctggttt accgcacctg ctaccgacgg gtgctcatta
    14221 aagtaagcaa taccaaagaa gccgacagcc accgcccctg ccaggcagag gatcatccag
    14281 gtcatactga tacgacgcgc atggacaatg ctgtggtgag aatccgccgc cataaaacgc
    14341 gccaggatgt gcggctgccc gaagtaaccc agcccccagc ccatcagcga gataatggca
    14401 acaaagttca gtcctttgag catatcgacg ttttcgatgc tcttttgttt gatcacttcc
    14461 agcgagtcgc caaacccacc aacgctaatg attacgataa ccggcgtcag gatcagggca
    14521 aaaatcatca ggctggcctg tacagtgtca gtccagctca ctgcaaggaa gccaccaata
    14581 aaggtgtaaa ggatcgtcgc cgcagcgccc gcccacagag ccgtttcgta gctcatgcca
    14641 aaggtacttt caaacagacg cgcgcctgcc acgatgcccg aagcgcaata aatggtgaag
    14701 aacagcaaaa tcaccagcgc ggagataatg cgcaaaatac ggcttttatc ttcaaagcgc
    14761 ccggtaaaat aatccggcaa tgttaaggcg ttattgttgt attcggtatg cacacgcaac
    14821 cgcccggcca ccagcttcca gttaatccac gcgcctaatg tcaggccaat ggcgatccag
    14881 ctttcggaaa tcccggaaag aaaaaccgcg cccggcaacc ccattaacag ccagccgctc
    14941 atatccgatg cacccgccga taatgccgtc acgaatggcc caaggctacg tccgcccaga
    15001 atatagtcgt caaagttttt cgttgatcgc caggcgataa acccaatcaa tatcatgcca
    15061 aagatataga cacaaaatgt caccaacatc ggtgtgctaa tagccatcta aagtctccaa
    15121 aaaattatta tcggcaatgt cgaaacttgc cgttatatct gccaccggaa cggggtagca
    15181 gagtttatgt tttaccaggg cgaccgtatc ctgccggaag cgctggttat tcacaatcga
    15241 tttaacacac catttacatt aaattttagt gctcagcgac actatttttc atcaggttgc
    15301 actctctcac attttttgcg gttgcacctt tcaaaaatgt taactgccgc agagaaaaag
    15361 tctgagttat ttttttaatc cctgtcatat cgatttcttt tattaacatt tcattcattt
    15421 ttaagcttgc tacgcaggtc acatttaaca tggttgcaca aagttgcaac atcatggata
    15481 tttcacgata acgttaagtt gcacctttca gaacaacagg agtaatggca tgggaaccac
    15541 caccatgggg gttaagctgg acgacgcgac gcgtgagcgt attaagtctg ccgcgacacg
    15601 tatcgatcgc acgccacact ggttaattaa gcaggcgatt ttttcttatc ttgaacaact
    15661 ggaaaacagc gatactcttc cggagctacc tgcgctgctc tctggcgctg ccaatgagag
    15721 cgatgaagca ccgactccgg cagaggaacc acaccagcct ttcctcgact ttgccgagca
    15781 aattttgccc cagtcggttt cccgcgccgc gatcaccgct gcctatcgcc gcccggaaac
    15841 cgaagcggtt tctatgttgc tggaacaagc tcgcctgccg cagccgattg ctgaacaggc
    15901 gcataaactg gcatatcaac tggccgataa actgcgtaat caaaaaaatg ccagtggtcg
    15961 cgcaggtatg gtccaggggt tattgcagga gttttcgctg tcatcgcagg aaggcgtggc
    16021 gctgatgtgt ctggcagaag cgttgttgcg tattcccgac aaagccaccc gcgacgcgtt
    16081 aattcgcgac aaaatcagca acggtaactg gcagtcacac attggtcgta gcccgtccct
    16141 gtttgttaat gccgcgacct gggggctgct ttttactggc aaactggttt ccacccataa
    16201 cgaagccagc ctctcacgct cgctgaaccg cattatcggt aagagcggtg aaccgctgat
    16261 ccgcaaaggt gtggatatgg cgatgcgcct gatgggcgag cagttcgtca ctggcgaaac
    16321 catcgcggaa gcgttagcca atgcccgcaa gctggaagag aaaggtttcc gttactctta
    16381 cgatatgctg ggcgaagccg cgctgaccgc cgcagatgca caggcgtata tggtttccta
    16441 tcagcaggcg attcacgcca ttggtaaagc gtccaatggt cgtggcatct atgaaggtcc
    16501 gggcatttca atcaagctgt cggcgctgca tccgcgttac agccgcgccc agtatgaccg
    16561 ggtaatggaa gagctttacc cacgtctgaa atcactcacc ctgctggcgc gtcagtacga
    16621 tattggtatt aacatcgatg ccgaagaggc cgatcgtctg gagatctccc tcgatctgct
    16681 ggaaaaactc tgtttcgagc cggaactggc aggctggaac ggcatcggtt ttgttattca
    16741 ggcttatcaa aaacgctgcc cactggtgat tgattacctg attgatctcg ccacccgcag
    16801 ccgtcgccgt ctgatgattc gcctggtgaa aggcgcgtac tgggatagtg aaatcaagcg
    16861 cgcgcagatg gacggccttg aaggttatcc ggtttatacc cgcaaggtgt ataccgacgt
    16921 ttcatatctc gcctgtgcga aaaagctgct ggcggtgccg aatctaatct acccgcagtt
    16981 cgcaacgcat aacgcccata cgctggcggc aatttatcaa ctggcgggtc agaactacta
    17041 cccgggtcag tacgagttcc agtgcctgca cggtatgggc gagccactgt atgagcaggt
    17101 caccgggaaa gttgccgacg gcaaacttaa ccgtccgtgt cgtatttatg ctcctgtcgg
    17161 tacccatgaa acgctgctgg cgtatctggt gcgtcgcctg ctggaaaacg gtgctaacac
    17221 ctcgtttgtt aaccgtattg ctgacacctc tttgcctctg gatgaactgg ttgccgatcc
    17281 ggtcactgct gtagaaaaac tggcgcaaca ggaagggcaa actggattac cacatccgaa
    17341 aattcccctg ccgcgcgatc tttacggtca cgggcgcgac aactcggcag ggctggatct
    17401 cgctaacgaa caccgtctgg catcgctctc ttctgccctg ctcaatagtg cactgcaaaa
    17461 atggcaggcc ttgccaatgc tggaacaatc ggtcgcggct ggtgagatgt cgcccgttat
    17521 taacccggca gagccgaaag atattgtggg ctatgtgcgc gaagccacgc cgagtgaagt
    17581 agaacaggcg ctggaaagtg cggttaataa cgcaccaatc tggtttgcca cgcctccggc
    17641 tgaacgcgcg gcaattttgc atcgcgctgc cgtgctgatg gaaagccaga tgcagcaact
    17701 gattggtatt ctggtgcgtg aagccggaaa aaccttcagt aacgccattg ccgaagtgcg
    17761 tgaagcggtc gattttctcc actactacgc cggacaggtg cgggatgatt tcgctaacga
    17821 aacccaccgt ccattagggc ctgtggtgtg tatcagtccg tggaacttcc cgctggctat
    17881 tttcaccggg cagatcgctg ccgcactggc ggcaggtaac agcgtactgg caaaaccggc
    17941 agaacaaacg ccgctgattg ccgcgcaagg gatcgccatt ttgctggaag cgggtgtacc
    18001 gccaggcgtg gtgcaattgc tgccaggtca gggtgaaacc gtgggcgcgc aactgacggg
    18061 tgatgatcgc gtgcgcgggg tgatgtttac cggttcaacc gaagtcgcta cgttactgca
    18121 acgcaatatc gccagccgcc tcgacgctca gggtcgccct attccgctca tcgctgaaac
    18181 cggcggcatg aacgcgatga ttgtcgattc ttcagcactg accgaacaag tcgtaataga
    18241 tgtgctggcc tcggcgttcg acagtgcggg tcagcgttgt tcggcactgc gcgtgctgtg
    18301 cctgcaagat gagattgccg accacacgtt gaaaatgctg cgcggcgcaa tggccgaatg
    18361 ccggatgggt aatccgggtc gcctgaccac cgatatcggt ccggtgattg atagcgaagc
    18421 gaaagccaat atcgagcgtc atattcagac catgcgtagc aaaggccgtc cggtgttcca
    18481 ggcggtgcgg gaaaacagcg aagatgcccg tgaatggcaa agcggcacct ttgtcgcgcc
    18541 gacgcttatc gaactggatg attttgccga attgcaaaaa gaggtctttg gtccggtgct
    18601 gcatgtggtg cgttataacc gtaaccagct gccggagctg atcgagcaga ttaacgcttc
    18661 cggttatggt ctgacgcttg gcgtccacac gcgcattgat gaaaccatcg cccaggtcac
    18721 tggctcggcc catgtcggta acctgtacgt taaccgtaat atggtgggcg cagtggtggg
    18781 tgtgcagcca ttcggcggcg aagggttgtc cggtaccggg ccgaaagcag gcggtccgct
    18841 ctatctctac cgtctgctgg cgaatcgccc ggaaagtgca ctggcagtga cgctcgcgcg
    18901 tcaggatgca gagtatccgg tcgatgtgca gttgaaagcc gcattgactc agccgctaaa
    18961 tgcactgcgg gaatgggcgg caaaccgtcc agaattgcag gcgttatgta cgcaatatgg
    19021 cgagctggcg caggcaggaa cacaacgatt gctgccaggg ccgacgggtg aacgcaacac
    19081 ctggacgctg ctgccgcgcg agcgcgtgtt gtgtattgcg gatgatgagc aggatgcgct
    19141 gactcagctc gccgccgtgc tggcggtggg cagccaggtg ctgtggccgg atgacgcgct
    19201 gcatcgtcag ttagtgaagg cattgccatc ggcagtcagc gaacgtattc aactggcgaa
    19261 agcggaaaat ataaccgctc aaccgtttga tgcggtgatc ttccacggtg attcggatca
    19321 gcttcgcgca ttgtgtgaag cagttgccgc gcgggatggc gcaattgttt cggtgcaggg
    19381 ttttgcccgt ggcgaaagca atatcctgct ggaacggctg tatatcgagc gttcgctgag
    19441 tgtgaatacc gctgccgctg gcggtaacgc cagtttaatg acgatcggtt aagcaggaag
    19501 ccggaggatg ttacaacctc ctccggcatc tttaacgtgg tcgaatcccc tcaataataa
    19561 tccgctgcac gttttcaacc gtttgattga aaaatacctc atcgcgcaac gtcgcgcctg
    19621 tcaccgcctc cacctgaggg gcgaaatcgg cgtaatgttg agtggaagcc caaatcataa
    19681 aaatcaaatg ctgcggatca atcggcgcga gtttgccgct tttgacccaa ccggcaatca
    19741 gcgccgattt ctcatcaatt aatgctttca aatcacccgt cagttcatcc attaacagcg
    19801 gtgcgcctgc cagcatctcc atacaaaaca gacgtgaagc ctgcggataa tcgcgtgaga
    19861 cttccagctt cagacggatg tactctttga tcgccgccag cggggcgaaa tcttcacgaa
    19921 acgcttttaa cggtgccagc cagatatcga gaatctgccg cagtacggct atatacagcg
    19981 cctcttttga cgggaagtaa tacagcaaat tggtttttga aacgcctgcc aactctgcga
    20041 tctgctccag ccttgtgccg tgaaaaccga attgtgaaaa agtgtccagt gctgcgctaa
    20101 gaatcgcttt tttcttcgcg ctcactgcgc gcgaacgttt acccgttgtt ttcactgcgc
    20161 cttgcgtcat gcgctctccc ctcttgtaga ttggcaacag gatagcaaaa cccctgcccg
    20221 ggctcgacct tctgcacttt catcgcgcac ggatgcatgt tttatgtgca actgttttga
    20281 ccgtttagtc cacttttttc cagatatttt agacaactta acattaacat attaataaca
    20341 ttcacttttt aaaaactggc atccgctttg caaacaaacc attacgcggc tgaaaaggat
    20401 atgcaggatg cagcgccccg tctgactttc actttacgag acgaagagag gttgatgatg
    20461 aaaattggcg tattcgtacc tattggcaac aacggctggc tcatttcgac ccacgcgccg
    20521 cagtacatgc cgacctttga actgaataaa gccattgtgc aaaaagcgga gcactaccat
    20581 ttcgacttcg ccctgtcgat gatcaaactg cgtggctttg gcggcaaaac tgagttctgg
    20641 gatcacaacc ttgagtcgtt caccttgatg gcggggctgg cggccgtcac ctcgcgcatt
    20701 cagatttacg ccactgccgc caccttaacg ttacctccgg caatcgtcgc ccgtatggcc
    20761 gcaaccattg actccatctc tggcgggcgt tttggcgtca acctcgtgac tggctggcaa
    20821 aagcctgagt atgagcagat gggtatctgg cctggcgatg actatttctc ccgtcgttac
    20881 gactatctca ccgaatatgt tcaggtgctg cgcgacctgt ggggcacggg caaaagcaat
    20941 tttaaaggcg attttttcac catgaatgat tgtcgcgtca gtccgcaacc gagcgtaccc
    21001 atgaaagtga tctgcgccgg gcaaagcgac gctggcatgg cgttctccgc tcagtatgcc
    21061 gatttcaact tctgtttcgg caaaggcgta aatacaccca cggctttcgc cccgaccgct
    21121 gcgcggatga aacaggccgc agagcaaacc gggcgcgacg ttggctctta tgtgttgttt
    21181 atggtgattg ccgacgaaac cgacgatgcc gctcgcacca aatgggaaca ctacaaagcg
    21241 ggcgcggatg aagaggcgtt aagctggcta accgaacaaa gtcagaaaga tacccgctcc
    21301 ggtactgaca ccaacgtccg tcagatggcc gatcccactt cggcggtaaa catcaacatg
    21361 gggacgttag tcggttctta cgccagtgtc gcgcgcatgt tagatgaagt cgcaagcgtg
    21421 cctagtgccg aaggcgtgct gttgaccttc gatgattttc tgtcgggaat cgaaaccttc
    21481 ggcgagcgca ttcaaccact gatgcagtgc cgcgcccatc tccctgcgct gactcaggag
    21541 gtggcatgat gacaatctta accgctcgac cggaagccat taccttcgat ccgcagcaaa
    21601 gtgcgctgat cgtggtggat atgcaaaacg cttatgccac gccaggcggc tacttagatc
    21661 tcgccgggtt tgatgtctca accactcgcc cggtcattgc caacattcaa accgccgtga
    21721 ccgcagcgcg agcggcaggg atgttgatta tctggtttca aaatggctgg gatgcacagt
    21781 atgtcgaggc tggcggcccc ggctcaccaa attttcataa atcgaacgcc ctgaaaacca
    21841 tgcgtaagca gccgcagcta caagggaaat tgctggcgaa aggctcctgg gattatcaac
    21901 tggtggatga actggtgccg cagcctggcg atattgtgct gccgaagccg cgctacagcg
    21961 gtttcttcaa tacgccgctg gacagcattt tgcgcagccg tggaatacgc catctggttt
    22021 tcaccggtat cgctaccaac gtctgcgtcg aatcgacgct acgcgatggc ttttttctgg
    22081 agtatttcgg cgtggtgctg gaagacgcaa cacaccaggc ggggccggaa tttgcacaga
    22141 aagccgcgtt attcaatatc gaaacctttt ttggctgggt cagcgacgtc gaaacgttct
    22201 gcgacgcgct ttctcccacg tcctttgctc gtatcgctta aggagtttaa cgatgccaaa
    22261 atccgtaatt attcccgctg gcagcagcgc accgctggcc cccttcgttc ccggcacgtt
    22321 ggctgatggc gtggtgtatg tctccggtac gctggctttt gatcaacata ataacgtgct
    22381 gtttgccgat gacccaaagg cgcaaacccg ccacgttctg gaaaccatcc gcaaggtgat
    22441 cgagacggcg ggtggcacga tggcggatgt gaccttcaac agcatcttta ttaccgactg
    22501 gaaaaattac gccgcgatta acgaaatcta cgccgagttt ttcccgggtg acaaaccggc
    22561 gcgattctgc attcagtgcg gactggtaaa acctgatgcg ctggtggaaa tcgccacaat
    22621 tgcgcatatc gccaagtgag gccgcgatga aactttcact ctcacctccc ccttatgctg
    22681 atgcgcccgt agtggtgttg atttcgggtc ttggcggtag cggcagttac tggttaccgc
    22741 aactggcggt gctggagcag gagtatcaga tagtctgtta cgaccagcgc ggcaccggca
    22801 ataatcccga cacgctggca gaagattaca gtatcgccca gatggcagcg gaactgcatc
    22861 aggcgctggt agccgcaggg attgagcgtt acgcggtggt cggccatgcg ctcggtgcgc
    22921 tggtgggaat gcagctggcg ctggattatc ccgcgtcggt aactatgctg gtcagcgtta
    22981 acggctggct acgaataaac gcccatacgc gccgctgttt tcaggttcgc gagaagttac
    23041 tacatagcgg cggcgcgcag gcatgggtgg aagctcagcc attgttcctc tatcccgccg
    23101 actggatggc ggcccgcgca cctcgcctgg aagctgaaga cgcgctggca ctggcgcatt
    23161 ttcagggcaa aaataattta ctgcgtcgac ttaacgccct caaacgcgct gactttagtc
    23221 gccatgcgga tcgcatccgc tgcccggtgc aaatcatctg cgccagtgat gatctgctgg
    23281 tgccatcagc atgttccagt gaacttcatg ccgccctgcc cgatagccag aaaatggtga
    23341 tgcgctatgg cggacacgcc tgcaacgtga ccgatcccga aacgtttaat gctctgttac
    23401 tcaacgggct tgccagcctg ttacatcacc gtgaagccgc ctgtaaggaa ttgctatgaa
    23461 cgaagccgtt agccctggtg cgcttagcac cctattcacc gatgcccgca ctcacaacgg
    23521 ctggcgggag acacccgtca gcgatgagac gttacgggag atttatgccc tgatgaaatg
    23581 ggggccgaca tcagctaact gttctccggc acggatcgtg tttatccgca ctgcagaagg
    23641 aaaagaacgt ctgcgcccgg cgctttccag cggcaatctg caaaaaaccc tgaccgcgcc
    23701 cgtcaccgcg atcgtcgcct gggacagtga attttatgaa cggttaccac agctgtttcc
    23761 tcacggcgat gcccgcagtt ggtttacctc cagcccacaa cttgccgaag aaacagcgtt
    23821 tcgcaacagt tccatgcagg cggcctatct gatcgtcgcc tgccgggcgc tggggctgga
    23881 taccggcccg atgtccggct ttgaccgtca acacgtggac gacgcctttt ttacgggcag
    23941 cacgctgaag agcaatctgc tgattaatat cggctatggc gatagcagca agctttatgc
    24001 gcgcctgcca cgtctgtcct ttgaggaagc ctgcgggctg ttgtaaggag caaactatga
    24061 acattgtcga tcaacaaact tttcgcgatg cgatgtcctg catgggcgcg gcggtcaata
    24121 tcatcaccac ggacggtcca gccgggcgcg ccgggttcac cgccagcgcc gtctgcagcg
    24181 tgaccgacac gccgcccaca ttactggtgt gtctgaatcg tggggcatcc gtctggccgg
    24241 tattcaatga aaatcgaacg ctgtgtgtaa atacacttag cgccggacag gagccgcttt
    24301 caaacctttt tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc cgctggcaga
    24361 ctggtgtgac cggatgcccg caactggaag aggcactggt ttcgtttgac tgccgtatca
    24421 gccaggtggt gagcgtcggc acccacgaca ttctgttttg tgccatagaa gcgattcatc
    24481 gtcacgccac cccctacggg ctggtgtggt tcgatcgcag ttatcacgcg ttgatgcgtc
    24541 ctgcttgtta acctccttaa ggagacagct gatggcaatg ttcggttttt ctcactggca
    24601 gttaaaatcg acctctacag agagcggcgt ggtcgcgccg gatgaacgat tgccgtttgc
    24661 tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga cggtgttaat
    24721 gcctattctg atggggctgg atcccaatct ttccattttg atgtcgggga tcggcacgtt
    24781 actgtttttc tttattaccg gcgggcgcgt ccccagttat ctcggctcca gcgcggcttt
    24841 tgtcggcgtg gtgatcgccg cgacgggctt taacggtcag ggcattaacc cgaatatcag
    24901 cattgcgctg ggcgggatta tcgcctgtgg gctggtttat accgtcattg gcctggtggt
    24961 gatgaaaatc ggtacgcgct ggattgaacg actgatgccg ccagtggtga cgggtgcggt
    25021 ggtgatggcg attggcctga acttagcgcc tatcgcagtg aaaagtgtat cggcttcggc
    25081 ctttgatagc tggatggcgg taatgacagt gctgtgtatt gggctggtgg cggtgtttac
    25141 ccgtggcatg atccagcgat tattgattct cgtaggatta attgtcgcct gtctgctgta
    25201 tggcgtgatg accaacgttt tagggctggg aaaagccgtc gattttactc tcgtcagcca
    25261 tgctgcctgg ttcggtttgc cacatttttc cactcctgct tttaatggtc aggcaatgat
    25321 gctgattgcg cccgtggcgg tgattctggt ggcagaaaac ttaggtcatc tcaaggcggt
    25381 cgccgggatg accggacgca atatggatcc atatatgggg cgggcattcg tgggcgatgg
    25441 gctggcaacg atgctttccg gctctgtcgg cggtagcggc gtgaccacct atgctgaaaa
    25501 tatcggtgtg atggacgtga caaaagtcta ctcgacgctg gtgtttgttg ctgctgccgt
    25561 catcgccatg ctgttgggct tttcgccgaa atttggcgcg ctgattcata ccattccagc
    25621 accggtgatt ggtggtgcat caattgttgt gttcgggctg attgccgtcg caggcgcaag
    25681 gatctgggta caaaaccgtg tcgatttaag ccagaacggt aatttgatta tggtcgcagt
    25741 aaccttagtg ctgggcgcgg gtgattttgc tctcacgctg ggcggtttta cgttgggagg
    25801 gatcggtaca gcaacctttg gcgcgatttt actcaatgcg ttgttaagca gaaggttggt
    25861 tgacgttcca ccgccagaag ttgttcatca ggagccataa atagaaaacc gccggggtaa
    25921 ccggcggtga tgactttttg acgagcttca gacaaaaatg catgcgtcat tgattagccc
    25981 ggctagttgt cgcttttgcc gtgactgctc ttgccacctt ttttacctgc ttcagatgcg
    26041 cgctgcgggt catttttgaa attacccccg ctgtgctgtc cacctttttt acctgcttct
    26101 gatgctcttt cgcggtcttc tgcaaaattg ccggaaccgc ctcgatggtt tgccattact
    26161 gacctccgtc tgggtataga catcatattg catagaatta acgcggcgta tggcggctgc
    26221 catattttcg ctacgtggga ctaagcttag tgaactgacg cgggcagacc cgcatttagt
    26281 aatatcctgc aacagcttct tagcgagaat agatatcatt tccaataatt atccataagc
    26341 cgctcttatg attaaaaaaa cgcaatgaga taaaaatgtg tcattttgcg acaaaattac
    26401 gtgcttgtga aagttgttat aaatcaaata agtggttgtg aaatttgcac tctgaaaagg
    26461 acgtcttatc tttaaataag tggtagcgaa tcgctacgga atagagataa cacgaggagt
    26521 ggttagaaat ggctaaagtt ctggtgcttt attattccat gtacggacat attgaaacga
    26581 tggcacgcgc agttgctgag ggtgcaagca aagtggatgg cgcagaagtt gtcgttaagc
    26641 gtgtaccgga aaccatgccg ccgcaattat ttgaaaaagc aggcggtaaa acgcaaactg
    26701 caccggttgc aaccccgcaa gaactggccg attacgacgc cattattttt ggtacaccta
    26761 cccgctttgg caacatgtcc ggtcaaatgc gtaccttcct cgaccagacg ggcggcctgt
    26821 gggcttccgg cgcactatac ggaaaactgg cgagcgtctt tagttccacc ggtactggcg
    26881 gcggtcagga acaaactatt acttcaacct ggacgaccct tgcgcatcac ggcatggtga
    26941 ttgtccccat tggctacgca gcgcaggaat tatttgacgt ttcacaggtt cgcggcggta
    27001 cgccgtacgg cgcaaccacc atcgcaggcg gtgacggttc acgccagccc agccaggaag
    27061 aactgtctat tgctcgttat caaggggaat atgtcgcagg tctggcagtt aaacttaacg
    27121 gctaatcttc aacaggagga tacgcatgcc aactcaagaa gcgaaagctc atcacgtcgg
    27181 tgaatgggca tctctgcgca atacgtcgcc ggaaatagcc gaggccattt tcgaagtcgc
    27241 cgggtacgac gaaaaaatgg cagaaaagat ttgggaagaa ggtagcgatg aagtgttagt
    27301 caaagccttt gctaagaccg ataaagattc gcttttttgg ggcgaacaga ccatcgaacg
    27361 taaaaacgtt taaccagcga ctcccccgta gcgaacgggg gagttttctg ttatttcacc
    27421 gcttcattca acacgctatc aaacttatcc atcggacaga aaccgttggc atctatcggg
    27481 caactgctta attccagcgt cacgcgctgc gcaggtgcct gaagggttaa cgcatcggca
    27541 ttacgtaact gttccgcact ctgataaaca tattcaattt tcatcaaatc gcgattggct
    27601 ttgctgtcat gccaacgctg gaaaacgatt ttgccgccaa tcggcgtgcg ttcgttctgg
    27661 tcatgcaact gatacggttt gaaatccagc gccgttaaca gagaggcaat gttggagtca
    27721 tgcccaacta acaccgtgat ttttggagcg ctggtgcgat cggtgaccag ggctttatcg
    27781 atataactga ccagcggttt cgcaacattg cgcgccacct ccggtgaggt aaacaggctg
    27841 tcctggtaac cgtttttcag cttcgacaac accttccact gctggtcaga tttgatttct
    27901 ccccaggcca cctgatccat cggaaaacct tcgtagtatt gcaaagtaaa cgcatctacc
    27961 agcgagttgc cgactttcag cggcccggaa acacctggtt cttgttgata cttcgcgcta
    28021 aaggtatttt tgccatccac cagcgaacat tgttgtttct ctttacaggc aggggaatct
    28081 ttatagttaa cgattttttc cagtagctgg tagctgtcgg taagctggag tttgctgagc
    28141 tctttctcca ttgccgccac cgcctgttca ctgaatgcgg cggaatcatc ggtgatcact
    28201 gggttaaagg ttgggtccat agtgcccatt ttttcctggt gatgcacagg aatatcacac
    28261 ccggggaatg cgccggtaat aaagaactgt gcagtcgcaa cggtacgttg cagactattg
    28321 gcataggcat aaacggtgtc cggcggcggg cattcccccg atttcaccat cccctgttct
    28381 gccagccatt cacgcatgta atggcccata tacacttcga gcacgccacc tttggtggtg
    28441 agttgcccac cggggacgtc ccattctggc catttattcg gcgttgactg ctccagcacg
    28501 ctgccattat tggccagcgg cgctcgtaag ttatggcggc tcatcatgag cacttgctgc
    28561 agctgatagc cttccggtac ggtttgtgcc tgagcatttg aagcgagtaa aaatatccct
    28621 gccacagctg cggcgattag cgttttgttc attcctggca cctcttttgt tatcaatcac
    28681 taaagagtgt gacagaaata tgactttttt ccgggaacag gttcgcaaaa tggcttgttg
    28741 aaaacctgtt tttctatata catcttcaaa aacaggcaag gtttgacgca aactatgcct
    28801 gattagcccc tgcctcgcgt acgccgctga taattgcacc cgtggctaaa cagaatatct
    28861 gcggcgttac caatacagag acaatattta acaataaaaa agtgtgagac taataacaat
    28921 ggtttttagc atatgccttt gccattaaat tgcataacga tttaaatagt caagttgcgc
    28981 attcatttcc ttattgttta acttgtaagc ctgagcaaaa agttcaggag ggaggtaaac
    29041 gtcataatga ttgccggtat ttattaaata accatactca agatcttcat ctgttgaaaa
    29101 ggattcattt cccaaggaga ttgatgccac attactatta acaataaata atgagcaaat
    29161 gctatctgaa agaacagcaa gaaaattctt caccgcttta atcctatccg acgtgtcggt
    29221 atctaaagca agcaaattct tgttatcatt aaatataaac tcgggtaaat gcaggataca
    29281 tttttcgtta ttcaagcaac tatcaatgaa ttctttttta aaaaacagaa agctccatga
    29341 cataagacta cccacaccct gaattttgtt gtcagattgc agatcaacgc caaataatgt
    29401 cttaacatct tccctgagat cacaaaatgc agcatcaaca agagcctcga ttgttggttt
    29461 atcaacaatt ttaataaact cttcgtatac ttccttttgc gtcttatgac tgcccagcgc
    29521 ccatgaataa tcatgttgtt ttaaaaccag tatggctctt aacagacaat atccatcgcc
    29581 atctactgga gcgtatttta attcataagt tttattaatt tcactctgaa taaaagatat
    29641 accagacaaa tcaataccag tatctaaatc agatattccc ggcgtattta tctgttctgg
    29701 ctggacactt atattttcat cttctaaaca atactcagaa aaaacatcat cacacgcatt
    29761 aatgttttca atatattcca taaagccata ccaataataa cctttaaaat ctgtgataga
    29821 ctctgaagct ctgcccggca aggacactct aacaacatca atatcttgaa tatattgtat
    29881 agtatatgtc atacctcctg aaacccctga ataaattttt gtatcattag tacgcaatgt
    29941 tctcaaaaat aatgataaat cattatcatt ctgcatttcc tcaaattctt tttctagtga
    30001 ttcgcattcc aggcatggaa tgtagtcttc agctttttga cgtctgattt ctttatacaa
    30061 ccgcataaaa cacaccagtg tgttatcaaa aagcgtgatt gaatatgttt gatttttttc
    30121 gtgctctttt tttatattgt gccaggcttg tttcaaataa ttatttgcag tgcaactaat
    30181 tccatgtata ttactaccca tatatagcgc ctataaaatt taataaataa tgacgcacta
    30241 gtaaaactta aagtgcctgg ttcaactatc aaaaatcgct cacccttttt cacctgttta
    30301 aaatatgttc agcaccccac cttgaggcga cctcctctcc gcgatgattt caataacata
    30361 ttctgtgttg gcatatgaaa ttttgaggat taccctacac ttataggagt taccttacag
    30421 gggttccttc aatttgtgtt gatttacgcg agataacgct atggaattaa aggattatta
    30481 cgccatcatg ggcgtaaaac cgacggacga tctcaagaca atcaagaccg cctatcgtcg
    30541 actggcccgc aaataccatc ctgatgtcag caaagaaccg gatgccgaag cccgcttcaa
    30601 agaggtcgct gaagcctggg aagtgttaag tgatgaacaa cgtcgcgctg agtatgatca
    30661 gatgtggcaa catcgcaacg atccgcaatt tagccgtcag ttccagcatg gagacggtca
    30721 gagttttaac gccgaagatt ttgacgatat cttctcgtca attttcggtc agcatgcccg
    30781 ccagagccgt caacgccccg ccacacgcgg ccacgatatt gaaatcgaag tggcggtatt
    30841 cctcgaagaa acgcttactg agcataagcg taccatcagc tataacctgc cggtttataa
    30901 cgcctttggc atgatcgaac aggaaatccc gaaaacgctg aatgtgaaga tcccggcggg
    30961 cgtcggcaat ggtcaacgca tccgcctgaa aggccagggg acgccgggcg aaaacggcgg
    31021 tccaaatggc gatttgtggc tggtgattca tattgcgcca catccgctgt tcgatattgt
    31081 cggccaggat ctggaaattg tggtgccggt tagcccgtgg gaagcggcgc tgggtgctaa
    31141 agtcaccgtt ccaacactga aagaaagcat tttgctgact atcccacctg gcagtcaggc
    31201 cgggcaacga ttgcgcgtta aaggcaaagg tctggtgagc aaaaaacaga ccggcgatct
    31261 gtatgcggta ctgaaaatcg tgatgccgcc gaaaccggat gaaaacactg ccgcgctgtg
    31321 gcaacaactg gcagacgccc agtcgtcttt tgatccacgt aaagattggg ggaaagcatg
    31381 atggctaatg ttacggtgac ttttactatt accgaatttt gcctgcatac cggcatctct
    31441 gaagaggagt tgaatgaaat tgtcggtttg ggagtggttg aaccgcgtga gatccaggaa
    31501 acaacctggg tatttgacga ccatgccgcc attgtggtgc aacgcgcagt acgcctgcgt
    31561 catgaactgg ctctggactg gccggggatc gcggtggcgc tgacgttaat ggatgatatt
    31621 gcgcacctga agcaggaaaa ccgcctgcta cgccagcggc tttcccggtt tgtagctcat
    31681 ccgtgatgtg tgtttgccgg atgtgatgac gcatccggca actgcgccct gatgcctgat
    31741 gcgacgcta
//
LOCUS       DAPOZO010000058        31558 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPOZO010000058 DAPOZO010000000
VERSION     DAPOZO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.058"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(354..620)
                     /gene="minE"
                     /locus_tag="ROI21_002828"
     CDS             complement(354..620)
                     /gene="minE"
                     /locus_tag="ROI21_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDX5111729.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(624..1436)
                     /gene="minD"
                     /locus_tag="ROI21_002829"
     CDS             complement(624..1436)
                     /gene="minD"
                     /locus_tag="ROI21_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDX5111730.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1460..2155)
                     /gene="minC"
                     /locus_tag="ROI21_002830"
     CDS             complement(1460..2155)
                     /gene="minC"
                     /locus_tag="ROI21_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDX5111731.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            2675..3043
                     /locus_tag="ROI21_002831"
     CDS             2675..3043
                     /locus_tag="ROI21_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HDX5111732.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3163..3564)
                     /locus_tag="ROI21_002832"
     CDS             complement(3163..3564)
                     /locus_tag="ROI21_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDX5111733.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            3635..3793
                     /locus_tag="ROI21_002833"
     CDS             3635..3793
                     /locus_tag="ROI21_002833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111734.1"
                     /translation="MAFLRSVKSRRGIFAGGSLIKGAWTEQTAEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            3805..4098
                     /locus_tag="ROI21_002834"
     CDS             3805..4098
                     /locus_tag="ROI21_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDX5111735.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4170..4829
                     /locus_tag="ROI21_002835"
     CDS             4170..4829
                     /locus_tag="ROI21_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDX5111736.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            4921..5367
                     /locus_tag="ROI21_002836"
     CDS             4921..5367
                     /locus_tag="ROI21_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDX5111737.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(5574..6485)
                     /gene="hlyE"
                     /locus_tag="ROI21_002837"
     CDS             complement(5574..6485)
                     /gene="hlyE"
                     /locus_tag="ROI21_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HDX5111738.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWKTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            6857..7276
                     /locus_tag="ROI21_002838"
     CDS             6857..7276
                     /locus_tag="ROI21_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDX5111739.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            7276..8544
                     /gene="umuC"
                     /locus_tag="ROI21_002839"
     CDS             7276..8544
                     /gene="umuC"
                     /locus_tag="ROI21_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDX5111740.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLAGTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(8590..9120)
                     /gene="dsbB"
                     /locus_tag="ROI21_002840"
     CDS             complement(8590..9120)
                     /gene="dsbB"
                     /locus_tag="ROI21_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDX5111741.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(9266..10807)
                     /gene="nhaB"
                     /locus_tag="ROI21_002841"
     CDS             complement(9266..10807)
                     /gene="nhaB"
                     /locus_tag="ROI21_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDX5111742.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            11029..11748
                     /gene="fadR"
                     /locus_tag="ROI21_002842"
     CDS             11029..11748
                     /gene="fadR"
                     /locus_tag="ROI21_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDX5111743.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(11800..13332)
                     /locus_tag="ROI21_002843"
     CDS             complement(11800..13332)
                     /locus_tag="ROI21_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDX5111744.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            13662..14960
                     /gene="dadA"
                     /locus_tag="ROI21_002844"
     CDS             13662..14960
                     /gene="dadA"
                     /locus_tag="ROI21_002844"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDX5111745.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            14970..16040
                     /gene="dadX"
                     /locus_tag="ROI21_002845"
     CDS             14970..16040
                     /gene="dadX"
                     /locus_tag="ROI21_002845"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDX5111746.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(16208..17644)
                     /locus_tag="ROI21_002846"
     CDS             complement(16208..17644)
                     /locus_tag="ROI21_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111747.1"
                     /translation="MASIKDIRPFAPGIPPSSVNPGVRPPGQFFLPVELKNEILRNMK
                     YIGPQIFSWGDDHRQIIDLSTAGEVKIHMVDNTIITLERLNPNPQHNEFMMVAEWCQW
                     AMERDGLQENLQKNLYEILEENQQNKQSEIPQEDLKESLEEIKENILEENLPASRIEN
                     RAEALRRMKECLITRRSMLNLSNLGLTSLPENLPPHLIEFYCSKNVLTALPKVMPKWL
                     LVLDCTDNVLILLPKVQPSKLMVLKCYENCIIWLPELSTNLRVINCSENFLQFLPPSM
                     PQYLYKLSCAGNNIHSIPDEMLENLTRLKVFDCSSNDLISAPRLPPKLIIYYCGENKF
                     KTVQVPQPQSLKVFDCNGNPWDKDNLPTLFKAVEGLKKQQGLKDLLGFLHKEGQMKLE
                     KLEKLEDLDDLMDLELLDDPVLLERVKVQEDLELLNQQLGLLILERQQDSQPVNQHSE
                     HEPESASRVKRDLSEVEFESTMKRKRFM"
     gene            complement(18135..19871)
                     /locus_tag="ROI21_002847"
     CDS             complement(18135..19871)
                     /locus_tag="ROI21_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDX5111748.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(19966..20880)
                     /gene="ldcA"
                     /locus_tag="ROI21_002848"
     CDS             complement(19966..20880)
                     /gene="ldcA"
                     /locus_tag="ROI21_002848"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDX5111749.1"
                     /translation="MSLFHLIAPSGYCIKQHDALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPQIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEPRTVT
                     LPLGAHAILTNTREGTQLTISGHPVLKM"
     gene            20980..21591
                     /gene="emtA"
                     /locus_tag="ROI21_002849"
     CDS             20980..21591
                     /gene="emtA"
                     /locus_tag="ROI21_002849"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDX5111750.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(21593..22327)
                     /locus_tag="ROI21_002850"
     CDS             complement(21593..22327)
                     /locus_tag="ROI21_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDX5111751.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDSKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            22528..22782
                     /locus_tag="ROI21_002851"
     CDS             22528..22782
                     /locus_tag="ROI21_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDX5111752.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(22832..24802)
                     /locus_tag="ROI21_002852"
     CDS             complement(22832..24802)
                     /locus_tag="ROI21_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDX5111753.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVATQESDSLTVWSSPVSSTTTTIL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(24828..25682)
                     /locus_tag="ROI21_002853"
     CDS             complement(24828..25682)
                     /locus_tag="ROI21_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HDX5111754.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     NPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            complement(25679..26491)
                     /locus_tag="ROI21_002854"
     CDS             complement(25679..26491)
                     /locus_tag="ROI21_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDX5111755.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPAHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALAAKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            complement(26501..27259)
                     /locus_tag="ROI21_002855"
     CDS             complement(26501..27259)
                     /locus_tag="ROI21_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5111756.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGVFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            complement(27256..28236)
                     /locus_tag="ROI21_002856"
     CDS             complement(27256..28236)
                     /locus_tag="ROI21_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDX5111757.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDIQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRH"
     gene            complement(28236..29258)
                     /locus_tag="ROI21_002857"
     CDS             complement(28236..29258)
                     /locus_tag="ROI21_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HDX5111758.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPTFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISLARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRTAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCPLSDADY
                     QKLVAS"
     gene            complement(29589..31286)
                     /gene="treA"
                     /locus_tag="ROI21_002858"
     CDS             complement(29589..31286)
                     /gene="treA"
                     /locus_tag="ROI21_002858"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDX5111759.1"
                     /translation="MKSPAASRPLKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPKGQSLREHIDGLWPVLTRSTESTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKASGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQNEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQKEAEPTP"
BASE COUNT         7858 a         7730 c         8048 g         7922 t
ORIGIN      
        1 taaactgcgc ttcggcgcag ttttcgttta cagggtgtta aaaggaaaaa ttctgcggca
       61 aaaaaagccc gccagttacg gcgggaaacc tcatcctatg ggagaacaat gaataatgaa
      121 attgcggggt tatcatcacc cagtataccc atactaacaa taaggttatt tactcagcca
      181 ggtataaaca atttgatctg tgtgggggga acggcattaa ggtgtaaagg ggggaagcaa
      241 tgagtagcat cagcaagaat actcgccgct cttatcaaac gtggatgagg gggatgaaaa
      301 acgcaaggca gagatgactc tgccttgaag agaaatgcgc ttttacagcg ggcctatttc
      361 agctcttctg cttccggcaa ggtcacgttc agctcaagaa tagaaatatc gccatctttt
      421 tgctcaagct gtacggttac catctcagga tcaatttgta cgtatttaca aatgacctca
      481 agaatatctt tacgcaactg cggcagataa tgcggttctg catcgctgcg acggcgttca
      541 gcaacaataa tctgcagccg ttcttttgca atgttggccg tgtttttctt ccgcgagaga
      601 aagaaatcga gtaatgccat aacttatcct ccgaacaagc gtttgaggaa gcctttcttc
      661 tcttcttcaa tgaagcggaa aggacgttct tctcccaaca gacgttctac ggtatctgcg
      721 taggctttac ccgcatcggc gttaatgtcg agaatgaccg gttcaccctg gttagaggcg
      781 cgcaatactg attgatcctc agggatcaca ccgacgagtt tgatgcgcag gatctccagc
      841 acatcttcca tgctcagcat gtcacctctg cttacgcggc ctgggttata gcgcgttaac
      901 agcaggtgct ctttaatagg ctcttcgcca ttttctgcgc ggcgtgattt cgacgccaga
      961 atgcctaaaa tacggtcaga gtcgcgtact gaggagactt ccgggttggt ggtaataatg
     1021 gcttcgtctg caaaatagag tgccattagc gcaccggtct caatccctgc cggggagtcg
     1081 caaacaataa attcaaagtc catcgctttc agatcatcaa gaactttggc gaccccttca
     1141 cgggtgaggg catctttatc gcgagtttgc gatgccggaa gaatatagag attttcagta
     1201 cgcttatctt taattaacgc ctgatttagc gttgcatcgc cctgaatgac gttgacgaaa
     1261 tcgtaaacga cccgacgttc acaacccata atcaggtcga gattacgcag gccgatatca
     1321 aaatctatca cgacagtttt ctttcccttc tgggccaaac cagtggcgat ggccgcgctg
     1381 gaggttgtct taccaacacc ccctttgccc gaagtaacaa caataatgcg tgccatagaa
     1441 attccttgtt aaaaagggat caatttaacg gttgaacggt caaagcgttt tcgactaact
     1501 gcagtcgcgc cgctttgcca taaaattctg ctgggatttg atcactcagc cagtattcac
     1561 ctgcgatgga caccagttcc gccatcaggt tcgtacaaaa tatttgcgtt tcccggtcac
     1621 cacttgcccc tgccagcgca cgaccgcgca tcatgccata gacatgaatg ttaccatcgg
     1681 caatcaattc ggccccagcg ctaacgtggc ttgtaacaat cagatcacat tgtggagcat
     1741 aaatacgctg accggaacgc accggggtat ctattaaacg cgtttttgtg accggcgttg
     1801 tattttgcgc tggagcctgc ggtgcgggag ctggacgcgg cgctttttcc tttccttccg
     1861 tcaggatagg cagccccatt ttttcaattt cggctttaag ttgcgcatct ttgcagccac
     1921 ttacgccaat aacccgcaaa ccggttgccg aaaccgcctt atgcatcgct gaccagttta
     1981 ccgggtcttc cagtgcactg acgttgagta caacgggggc atgttttaaa aatgcggggg
     2041 cctgagcgat tttgtcttcc agcgcctgat ggataacctt aggttctgcc tcatgcagat
     2101 gaaccacaga taaagtgaag ctactgcctt taagctcgat tggcgtgttt gacatcctgg
     2161 ccttactcaa ttagctatta atcatcgcca gcgcgcgatg atgttccgaa gactataagg
     2221 catgttatag tctggattat attgaggcaa gtcaccctcc catttattca gagtaaaagt
     2281 ctattctgtg ataaatggcg ctgattcata gcttaaaaaa tacccttgtc aatcaaccca
     2341 ttgccgtcgc acttttgatt gttcttattt acgcttcctt tttccgcacc ctaatcgtga
     2401 cagcatcttc aggcctgttt aaatatccag tattgaggca agtgtatttt ttcaccacgc
     2461 caggctttct taactttcca ttttttgcgg catcattaaa gaacagatac ccgttatctt
     2521 aatatccgcc acagaattgc tctatcatag aaaccgcaga agacgtctga tacttctgtc
     2581 aggtcatcgt aactcgtcca ggtcagcggt tttgattacg gattgatgat gaaatgagca
     2641 taagtgttgt acggttggat tatcaggatg tgctatgaat atgatgcgta ttttttatat
     2701 cggattgtca ggtgtgggga tgatgttctc atcaatggcg tctggaaatg acgctggcgg
     2761 acttcaatct ccggcgtgcg gcgttgtttg cgacccgtat atttgtgtta actcagatgg
     2821 catttcccca gagttaacaa ggaaatatct cggcgaaaaa gccgctgaaa acttacaatc
     2881 attacaaggc tacgatccca gcgaatttac attcgctaac ggtgtatttt gcgatgttaa
     2941 agaaaaatta tgtcgtgatg atcgttattt tggtgtggat ggtaagcgga gtggaaaaat
     3001 caatcaaacc accacaaaga tgttatttat gtgtcgtgag tgatgccttg tttacattat
     3061 gttctattcg gcattgtctg ctattcgtca ttaacctctt gagcgacaga gaggagtggg
     3121 gcgttaacgt ttatgacggg gagagtcccc gtcggttgac atttacttaa tttgaatatc
     3181 gacgttatat tttttagttt tgtttttacg ggcgtcgttt cgggtttgca gcactcgcag
     3241 ctcatatttg ccagaagcag ggagtgagta ttgtccgtga ctatcgagtt ctggggaata
     3301 tctggataaa tcaacagaat catcgattcc tggcccgaag aggtaagtat cagcgccttc
     3361 attagagata ctcacatgca ctttctgacc ttttttggca tagaaggtat atgtatcgta
     3421 atcgtatccc tttatttcgc cggagtactg agcgctgcta tggccttttc tgaactcaac
     3481 attgacattc ttacccgcag caaagctcgt ggaggttagc agtgcaagca ataatacagc
     3541 cttactgatg cccttgattt tcataggtag tctcctgttc agaatgagga cgatgaactg
     3601 ttataatata acaattcatc acaggagaat tccagtggct ttcctgcgtt cggttaaatc
     3661 ccggcgcggc atttttgccg ggggatcact tattaagggc gcatggactg aacaaaccgc
     3721 agaaatctac agctggctgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa
     3781 cctggcatct taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac
     3841 cagacctatt tatatgtcga aaaaaaagac gatttttcgc gtgtacctga ggaactgatg
     3901 aaaggttttg gtcagcctca gttagcgatg attctgccgc tggatgggcg gaagaaactg
     3961 gtcaatgccg atattgaaaa ggttaaacag gcattaaccg agcaaggtta ctatttgcag
     4021 ttaccgccac cacccgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca
     4081 gacgacacga acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt
     4141 catatccacg ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc
     4201 tgctggatta tccggtgagt aaagtagtct gtgttggtag taactatgcc aaacatatta
     4261 aagagatggg cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac
     4321 tgtgcgatct gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag
     4381 tcgaactggc ggtattgatt ggcgcgacgc tgcgtcaggc tacggaagag catgtccgta
     4441 aagccattgc cggttacggc gtggcgctcg atctgacatt gcgtgatgtt cagggaaaaa
     4501 tgaagaaagc cgggcagccg tgggaaaagg ccaaagcgtt tgataactct tgtccgcttt
     4561 ccgggtttat ccccgcggcg gaattcaccg gcgatccgca aaatacaacg ctgggcctga
     4621 gcgtaaacgg cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc
     4681 cgctgatcgc ttatatgagc aagttcttta ccctcaaggc cggtgacgtt gtgctgacag
     4741 gcacgcctga tggcgtcggc ccgttgcaaa gtggtgatga gctgacagtc actttcgatg
     4801 ggcattcttt aacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa
     4861 cttgcatcgc tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg
     4921 atgagcgatg tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg
     4981 gagtcgttgt gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc
     5041 gacgaaatct acttcactaa cgtcgcctgt cgccagctca atattaaaac ctgtcaatgt
     5101 cggaactacg aacgtcgttt cgagtttgaa cctgactgca ttaaattaac ccgtgaaaat
     5161 ctgccaacat tcgaatggct gccaatgacc tgcgcttatc gcttgctggc ggaaggtaaa
     5221 gatttacctg cgtggcatcc gctgcttact ggttcgaaag cggcaatgca tggcgaacgt
     5281 atctctgtgc gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc
     5341 ttaaataaac ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta
     5401 atatacgtct ggatactcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg
     5461 agagttaccc gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtat
     5521 ttttaaatgt gataagcttt ataccttgag tacatggaga gaataatcgc ttatcagact
     5581 tcaggtacct caaagagtgt cttcttaccg tgtcttttct gatactcatt acaggtgtta
     5641 atcatttttt tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag
     5701 aatctggttg tttcagtttc cgtttttatc tccccgatgg cggctatttc ggtggttaat
     5761 ttcaatttgg cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta
     5821 aagaaactct gcacagattt taacttgttc ttcaattctg gaatcagttt cccttcaact
     5881 acgcccgcag caatagaata ggaaatgatt aatccaaatg gaccggcgac gacaccggct
     5941 gcggcaccgg catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt
     6001 ttttctgaaa aatcattggt taactggcta tctaacgcca gcagttttcc ggaagcgttg
     6061 ttgaaacttt gtgagcttac cagcagagat ttttgcgctt cattcagctt cgtgatgccg
     6121 tcatccagta ccttaatgag aatgtctttc tgggcggatg ctttcttctc attgtactca
     6181 tcaaatagca aaatatatgc tgcgagcaat tgcgtcgcaa caccacacca ttcatacacc
     6241 gtttgggttg cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgcca
     6301 actaaaacgg aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt
     6361 tcatcgaagg tcttccaggg gatgacctga tcgagatatt tattataaag gtctaatgct
     6421 ccatctgcgg tttcgattgc gtttttaact acctctaccg ttttatctgc aacgatttca
     6481 gtcataatca ttcgcctctt taaatatata aattgtaatg aaactcctgt tttacaacta
     6541 ttaatgaatt ttacttcatc taattcatag ttaggcgggc gagatgcgtc aatgtcttta
     6601 tttctattaa tatgataaat atcaaacaat gtttaatgtc attatggcga atgcttctat
     6661 tctatttttt agccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg
     6721 cgcgtgagca caggataaga gaacgaaaaa tcagcagtct atgcagcgac aaatattgat
     6781 agcctgaatc agtattgatc tgctggcaag aacagactac tgtatataaa aacagtataa
     6841 cttcaggcag attattatgt tgtttatcaa gcctgcggat ctccgcgaaa ttgtgacttt
     6901 tccgctattt agcgatcttg ttcagtgtgg ctttccttca ccggccgcag attacgttga
     6961 acagcgcatc gatctgaatc aactgttgat ccagcatccc agcgcgactt acttcgtcaa
     7021 agcaagtggt gattctatga ttgacggtgg gattagtgac ggtgatttac tgattgtcga
     7081 tagcgctatt accgccagcc atggtgatat tgtcatcgct gctgttgacg gcgagtttac
     7141 ggtgaaaaaa ttgcaactac gcccgacagt gcagcttatt cccatgaaca gtgcatattc
     7201 acccattacc atcagtagcg aagatacgct ggatgtcttt ggtgtggtga tccacgtcgt
     7261 taaggcgatg cgctgatgtt tgccctctgt gatgtaaacg cgttttatgc cagctgtgag
     7321 acggtgtttc gccctgattt atggggtaaa ccggtggttg tgctatcgaa taatgacggt
     7381 tgcgttatcg cccgaaacgc tgaggcaaag gcgttgggcg ttaaaatggg cgatccctgg
     7441 ttcaaacaaa aagatctgtt tcgccgctgt ggcgtggttt gctttagcag taactatgag
     7501 ctttacgccg atatgagcaa tcgggtgatg tcgacgctgg aagagctatc gccccgcgtc
     7561 gagatttaca gtattgatga ggcattctgc gatctgacag gtgtgcggaa ttgtcgcgat
     7621 ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca tcttactgtt
     7681 ggcgtgggga tcgcccagac caaaacgctg gctaagcttg ccaatcatgc ggcaaaaaaa
     7741 tggcagcggc agacgggtgg ggtggtggat ttatcaaatc tggaacgcca gcgtaaatta
     7801 atgtctgctc tccccgtgga tgaagtctgg gggattggac ggcggatcag caaaaaactg
     7861 gacgcgatgg ggatcaaaac cgttctcgat ttggcgggta cagatatccg gtttatccgt
     7921 aaacatttta atgtcgtgct cgaaagaacg gtgcgtgaac tgcgcggtga accctgtttg
     7981 caactggaag agtttgcacc gacgaagcag gaaattatct gttcccgctc gtttggtgaa
     8041 cgcatcacgg attatacgtc gatgcggcag gccatttgta gttacgctgc ccgggcggcg
     8101 gaaaaacttc gcagtgagca tcaatattgt cggtttattt ccacgtttat taagacgtca
     8161 ccatttgcgc tcaatgaacc ttattacggc aatagcgcgt cggtaaaact gctgacgccc
     8221 actcaggaca gcagggatat cattaacgcc gctacgcgat ctctggatgc catctggcaa
     8281 gcgggccatc gttaccaaaa agcgggcgtg atgctggggg atttcttcag tcagggagtc
     8341 gcgcagctca atttattcga tgacaacgca ccgcgccccg ggagtgagca attgatggcg
     8401 gtaatggata cgctaaatgc taaagagggc agaggaacac tctattttgc cgggcagggg
     8461 atccagcaac aatggcagat gaagcgtgcc atgctttcac cacgttatac aacgcgaagt
     8521 tctgatttac tgcgggtcaa ataaacatag cggcaggaaa aaagcgctcc cgcaggagcg
     8581 ctgaatggat tagcgaccga acaggtcacg ttttttcgct ttaaacggct gggaaatcac
     8641 caccagcacc gcgacaatca ggtaagcgat aaaaataccg agcaaccact gcggcatttc
     8701 cagacctaaa aaatcccact gacgctcggc gcaatcgcca gaggcgacaa acacttgcgg
     8761 cacccattta tccagcggca gccattccgg gaaacgaacc ataaaatcac aggtcgcaaa
     8821 cggcgaagga tagagctgaa gcatggtgtg ctcgtaagtt aactgcacac cgcggaacgc
     8881 actatacaac cagataacca tcgctacata acgcagcgga gttttcgggg cgatcgcgcc
     8941 aatcagcgca gcacccagaa cgccgaataa tgcgcagcgt tcataaatac agagcacgca
     9001 aggtttcagt aacatcacat gctggaacca cagtgccgtc agttccagtg ccagagcggt
     9061 aaacgccatc aacagccacg cgccccggcc ttgtgaacat tggttcaaaa atcgcaacat
     9121 aatcatttcc ctgcaatatg catagagtgc gcagtttaaa ccaattcatt cgctgcgcca
     9181 ccaggaggca gcaaaaatag gacgcaattg gacaattatc gggcgaaaag gcaaaccggg
     9241 catttatatg cccggtaagt tgttatcaaa gcgttgctat ccagcccatt tgcataaacc
     9301 attcggttac aggggcaagc gtaaactcga cgcaaagtag tccgacgagt gtcaggacga
     9361 gggtgtaagg cagggccatc cacaccatgc ggccataaga gaggcgaatc aatggcgcga
     9421 gtgcagaggt cagcaggaac aggaacgcag cctgaccgtt cggcgtagcg acagagggca
     9481 gattggtacc ggtattaatg gcgaccgcca gcagctcgta ttgcttcaac gtgatagcgc
     9541 cactttccat tgccgctttc gcttcgttga tataaatcgt ccccacgaag acgttatccg
     9601 aaatggatga cagcagaccg ttgaaaatat agaacagcga cagctgagca tgttccgatg
     9661 cctgcaacac aaactgaata atcggcgaaa acagttgttg gtcgataatc accgcgacta
     9721 ccgagaaaaa caccgtcaac agtgcggtga atggcagaga ttcggtgaag gctttaccga
     9781 tagcatgttc atcggtgaca ccggtcaatg atgtcgccag aataatgacc gacaaaccaa
     9841 tcaagccaac ttccgccaga tgcaacgcca gcgcagtcac cagccagacg ccaataatcg
     9901 cctggacaat cagacggatt ttatcctgac gggtacgctg gtggcggctt tgatcgtcaa
     9961 actgttgcag tacttcgcgg actttctccg gcagcgtttc accatagcca aaccaacgca
    10021 gcttctctac cagcaggcag gttaacaggc cacaaatcag aaccggaacg gtcactggcg
    10081 acatgcgcag gaagaaatcg ccaaaatgcc agccagccgc tttagcgatg atcaggttct
    10141 gtggttcgcc caccatggtc atcacgccgc ctaatgcggt gccgacaccg gcatgcatca
    10201 tcaggctacg cagaaagcca cggaactgtt ccagaaccac tttgtaatgc ttgtcgatat
    10261 gactatcgtc ttgcaggtcg gtatcttcgg tacgggaaga ggctacgcgg tgataaatcc
    10321 cataaaaacc gactgcaacg ctgatcacca ccgccacgac ggttaaggca tcgaggaacg
    10381 cggagaggaa cgccgccgcc acgcaaaaag agagcgacag cagcattttg gagcgaatgc
    10441 ttaacagcaa acgggtaaat atgaacagca atagctgttt cataaaatag atacccgcca
    10501 ccataaacat cagtaacagc aagacttcaa gatttgccgc cacctcttca cggacgtgtt
    10561 ccgcgctggt catgccgatg aataccgctt cgatagccaa cagaccaccg gggagcagcg
    10621 ggtagcattt cagggccatc gccagagtga aaataaattc cgctaccagc aaccagcccg
    10681 cgacgaaagg gctgatgagg aaaattaacg gatttacgat taagaaaatg atgagggcga
    10741 gtttgtacca gtcgggggac tggcccaaaa aattgcgcca taacgcgcgg ccccaggaga
    10801 tctccatgat ggtttccctt accttacaaa taatcaatga tgtttttatg tttaaacgca
    10861 aagcttaacg gtcaggcagg agtgaggcaa gtcttgatag tcaagggtaa agagatgcgg
    10921 aaaatgaagc cttgatccct tttttcttct ttttgtctgc tatcagcgta gttagccctc
    10981 tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat ggtcattaag
    11041 gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg gaataaccgc
    11101 ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat tggcgtaacg
    11161 cgtactacgt tacgtgaagt gttacaacgt ctggcacgag atggctggtt gaccattcaa
    11221 cacggcaagc cgacgaaggt gaataatttc tgggaaacct ccggtttaaa tatccttgaa
    11281 acactggcac gactggatca cgaaagtgtg ccgcagctta ttgataattt gctgtcggtg
    11341 cgtactaata tttcaacgat ttttattcgt accgcgtttc gtcagcatcc tgataaagcg
    11401 caggaagtac tggcaaccgc taatgaagtg gccgatcacg ccgatgcctt tgccgagctg
    11461 gattacaaca tattccgcgg tctggcgttt gcttccggca acccgattta cggtctgatc
    11521 cttaacggga tgaaagggct gtatacgcgc attggtcgtc actatttcgc caatccggaa
    11581 gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag tgaaggcgcg
    11641 cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggcga gatttggcac
    11701 cggatgcaga aaaatttgcc gggtgattta gccattcagg ggcgataatc ccttccgttt
    11761 aaaaagcaaa cccctcaaac gaggggtttt ttgttgtttt tacagatttc ccattctcgg
    11821 cgggcaacgt tccagcaact cgacgctgcc atcttcgttt tgctgttcga gcatcacatc
    11881 aaatccccac aggcgatgca catgcttcag tacttctttg cgcccccgat ccagcggtgc
    11941 gcgattatgt ggaatgtaac gcagcgtcag cgaacggtcg ccgcgcaaat ccacgttcca
    12001 gatctgaata ttcggctcca gattacttaa attatattga gacgataacc ggttacggat
    12061 ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat gccgatcgtc
    12121 atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagga actggctgat
    12181 aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc agtcggaacc
    12241 ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa tccgtttaat
    12301 atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc acgggctgtt
    12361 atagggcggc tggaagacca cattggtgtg gctgtgcaaa aactccagca taaaacgttc
    12421 cgttactttc ccttcatcat acagatggtt aaggatggtg tagtgccaga aggtcgccca
    12481 gccttcgttc atcacctgag tctgtttttg cggataaaaa tactggctca ccttacgcac
    12541 aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct ccataaaata
    12601 gagcaggttt tcttgtggct cggacggata gcggcgcgct tcagcaaccg ttttctcttc
    12661 ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgcagat actcttcgcg
    12721 acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt tgtaccggtc
    12781 cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt catcaacgcc
    12841 ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat cgacaatcga
    12901 actggcgtcg gtccagctac ggaataagta attgtttttg aagaaagagt tatgcccata
    12961 gcaggcgtgt gccatcacca gcgcttgcat ggtaatggtg ttttcttcca tcaggtaagc
    13021 gatacacggg ttagagttaa tgacgatttc ataggccagt ccttgctgac cgtgcttata
    13081 cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt taatgggcat
    13141 gccgacgctg gagtaggcat ccatcatctg ttctgaggtt atcacttcaa tctggtgcgg
    13201 gtaggtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat aaacatccag
    13261 caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg gtgtccttat tcatagaatc
    13321 gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag cacctcattt
    13381 caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt tgcgatttct
    13441 tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat aaaaagggcg
    13501 ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg tgagccagct
    13561 caccataaaa aagccgtatg ttgaataata tttttaactg agttatcaag atgtaattag
    13621 attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt gtcatactgg
    13681 gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga catgaggtca
    13741 ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat gccgggcaaa
    13801 tctcccccgg atatgcagca ccgtgggcgg caccaggtgt gcctttaaaa gcgattaaat
    13861 ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag ttccagttga
    13921 aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa aacaaagggc
    13981 ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc gccgaaacca
    14041 atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc gaacaacagt
    14101 atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg taccagctgc
    14161 tggaatccag ccgtctggcg gaagtggagc ccgcgctggc agaagtggcg cacaaactga
    14221 cgggggggct ccagctgccc aatgatgaaa ccggagactg ccagctgttt acccagaatc
    14281 tggcgcggat ggcggagcag gcgggggtta aattccgctt taatacgccc gttgaccaac
    14341 tgctttgcga cggcgagcaa atctacggcg tgaagtgtgg cgatgaagtg attaaggccg
    14401 atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc atagttgata
    14461 ttccggtgta tcctttgaaa ggctactcgc tgaccattcc cattgcgcag gaagatggtg
    14521 cgccggtatc caccattctt gatgaaacct acaaaatcgc cattacccgt ttcgataacc
    14581 gcattcgtgt tggcggaatg gcggaaattg ttggttttaa taccgagctg ttgcaaccgc
    14641 gtcgtgaaac gctggagatg gtggttcgcg atctctatcc acgcggcggt catgtcgagc
    14701 aggcgacttt ctggactggt ctgcgcccga tgacgccaga cggcacgccg gttgtcgggc
    14761 gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc tggacgatgg
    14821 cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca gcgatcccgt
    14881 atgaagatct aagcgtagcg cgctacagcc gtggatttac gccatcacgc ccgggccatt
    14941 tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc agcctcgatc
    15001 tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac gcgcgcgtct
    15061 ggtcggtggt aaaagcgaac gcttacgggc acggtattga gcgtatctgg agcgcgctcg
    15121 gggccaccga tggctttgca ttacttaacc tggaagaggc aataacgtta cgtgagcgcg
    15181 gctggaaagg accgatcctg atgctggaag gatttttcca tgctcaggat ctggagattt
    15241 atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa gcactgcaaa
    15301 atgcacggct aaaagccccg ttggatattt atcttaaagt gaacagtggg atgaatcggt
    15361 tgggcttcca gcccgatcgc gtgcttaccg tctggcagca gttgcgggcg atggcgaatg
    15421 ttggcgaaat gaccctgatg tcgcattttg ccgaggcgga acatcctgat ggaatttcca
    15481 gcgcgatggc gcgtattgag caggcggcgg aagggctgga gtgtcggcgt tcgttgtcca
    15541 attcggcggc gactctgtgg cacccggaag cgcattttga ctgggttcgg cctggcatta
    15601 ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatacc ggattacgtc
    15661 cggtgatgac gctaagcagc gagattattg gtgtccagac gctaaaagcg ggcgagcgtg
    15721 tgggctacgg cggtcgctat actgcgcgcg atgaacagcg aatcggcatt gtcgccgcag
    15781 gctacgccga cggttatccg cgccatgcgc ctaccggtac tcctgtttta gtggacggcg
    15841 tgcgcaccat gacggtgggg accgtctcga tggatatgct ggcggttgat ttaacgcctt
    15901 gcccgcaggc ggggattggt acgccggttg agctgtgggg caaggagatc aaaattgatg
    15961 atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcgctg gcgttacgtg
    16021 tcccggttgt gacagtgtaa cttgttgtaa gccggatcgg aggcagcgtc ttctggttgc
    16081 gaaaaaatca tccatccggc tggtcagcaa ctgtagttgt tgatgtgaca gagccatttc
    16141 ccatgatagt gtccattaca atgatggaca ctatttcccc ggaacctgag ttcaccgcac
    16201 aggcgttcta cataaaacgc ttacgtttca ttgttgactc gaactcgact tcagataaat
    16261 cacgcttcac ccttgatgca gattcgggtt catgttcaga atgttgatta acaggctgcg
    16321 agtcctgttg tctttccaga atcaacaggc ccaactgctg attcaggagc tccaggtcct
    16381 cctgtacctt cacgcgctcc aggagtaccg ggtcatccag gagctctaga tccataaggt
    16441 catccagatc ctccagcttc tccagcttct ccagcttcat ctggccctcc ttgtgcaaaa
    16501 agcccaaaag gtccttcagg ccctgctgtt ttttcaggcc ctcgacggct ttgaacagcg
    16561 tcggtaaatt gtctttgtcc cacgggttac cattacagtc aaacaccttc aggctctggg
    16621 gctgtggcac ctgtaccgtt ttaaacttgt tttctccaca gtagtaaatt attagtttgg
    16681 gcggcagccg tggtgcagag atcaaatcgt tactagaaca gtcaaatact ttcaggcgag
    16741 tcaagttctc cagcatctca tcaggtattg agtgaatatt gttgccggca caggatagtt
    16801 tatacaggta ctggggcatc gacggcggta aaaattgcaa gaagttttca gaacagttaa
    16861 tcactctcag gttagtcgac agctcaggca gccagataat acagttttca tagcacttca
    16921 gtaccatcag ttttgagggc tgcaccttcg gtaacaagat caagacgtta tctgtacagt
    16981 caagcaccag cagccacttt ggcatcacct tcggtaacgc ggtcaacacg tttttactgc
    17041 agtaaaattc aatcagatgt ggaggcaaat tttcagggag tgaagtcaac ccaaggttgc
    17101 taagattgag catactccgt cttgtaatga gacattcctt cattctgcgc aatgcttctg
    17161 cccggttttc aatccggctt gctggcaggt tttcttccag gatattttcc ttgatttctt
    17221 ccagactttc tttcaggtct tcttggggaa tttctgactg tttgttttgc tggttttctt
    17281 ccaggatttc gtacaggttt ttttgcaggt tttcttgcaa accatcccgc tccatagccc
    17341 attggcacca ttcggcgacc atcatgaact cgttatgttg cggattcgga ttcaggcgct
    17401 ccaacgtgat gatggtattg tctaccatat gtattttcac ttctcccgcg gtactgaggt
    17461 caatgatctg ccggtggtca tcaccccagc taaatatttg cgggccgata tacttcatgt
    17521 tccgcaaaat ttcattcttg agttccaccg gcaggaagaa ctgccctggt gggcgtacgc
    17581 ctgggttaac ggaagatggg ggaatgccgg gggcgaatgg gcggatgtct ttaatgctgg
    17641 ccataatgct acgtatcctt gaaataaata tcctctggca gcgccggaga cgtgaatgtg
    17701 gtcaccgcaa aggtgccaca ggacgtcact tgcgctgata taacgactca tcgttgactt
    17761 tttcccttgg tcagaaatgc cgtgtcgggt ctggtacatg ctgtattacg gcccaggcag
    17821 ctctgacgta ttaaagaaaa tacgagagcg aacgacccgg aaataatcgt tattaagcaa
    17881 aggaataact ataagaaatc gctcatgcat ccggtgcttc ttcacctgtt gcagtcagaa
    17941 gaagccatta cgctgacttt cttgagccgg tgttttgagt tgattggcca gagcctgcaa
    18001 tggtttgtca tccataattt aaccttcttc tgatgttgga atgtgcatat caaaatcagg
    18061 aaatgacaag tttatatgca ggctcaggct gcgttcgcaa agcatcgcat ccggcgatag
    18121 atgctgtatt aaatttaaga ttcagcttcc tcttctgcca cccgtacgcc aatcttcaac
    18181 acttcattat cttctttctc ggccaccgtc cagatcattc cggcaaactc tacctggtct
    18241 ccgacaaccg gtgccgcgcc taacaactgc tggacaattt cacccagcgt ttgctgctta
    18301 tcacgatatt ctcgcccgtc ttccaggcca tatatcagcg ccacatcagc atatttagcg
    18361 ctggcttcga gaatgaagtc accaaagaag cgttgatcca gcgcgacagg tggcgactgg
    18421 ctgaacagtt taccgagcgc cgggagatcg cgttcccgac caattacaca caacacatcg
    18481 ccttcgcgca gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagtgcc
    18541 gcaatacgcg tctcttttgg catatggaga tcacgcagtg ccgcgcccac gcaccattta
    18601 tcggcactca actgataaac aaactgctcc cacggatttt ccggatgaat atccagacca
    18661 acgcgtgaca ccggacgtcc cactggcgga acgaccactt tggctttttt cgccgcccac
    18721 gagagtgatg tcccctgcaa gagtagtgaa accagaacca caaagaaggc gacattaaag
    18781 aacagacgcg cattctccag ccccgccatc atcgggaaca cggccaggat gattggcacc
    18841 gcgccgcgta atcccaccca gctgataaac acgcgctcac gcagattgaa gccacgaaac
    18901 ggtagcaatc cggcaaatac cgaaagagga cgggcgaaga aaatcatcca tgcggacaaa
    18961 atgagcgccg gaatggcaat aggcagcaga tcgcttgggt taaccaacag ccccagcacc
    19021 aggaacatgg cgatttgtgc cagccaggca aggccgtcga aattttgcag gatgccgtag
    19081 cggttgcgaa tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg
    19141 ctaccttcca gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat
    19201 ggatataatc cggcgggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg
    19261 ccaagaccga tgacaatccc gaggccaaat tgctgcagaa tatcgactac gaacatccaa
    19321 ctgacactgc tttcatgttg ctggatcatc gcaatcaggg taattgtcag aaagaccgcc
    19381 attggatcat tactgccgga ttcaatttcc agcgtcgagc caacgcgttc gttaagcccc
    19441 ttaccaccca gcagagaaaa taccgctgca gcatcggtgg agccgacgat agcgccgatt
    19501 aataagcctt caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca
    19561 gaggtgataa gcacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag
    19621 gaactggcct gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt
    19681 accatgtagg cgaagggata attatcaaac gggataccgc cgacgccatc gactcccgcc
    19741 agcatgccga tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa
    19801 ctaagtaata tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt
    19861 gtggcatcca acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat
    19921 tagcggcaga gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac
    19981 aggatgaccg ctaatcgtca actgagtgcc ttcccgggtg ttagtaagaa tggcatgtgc
    20041 gcccaacggc agcgtaaccg tgcgtggttc atgaccaaaa tcgaggccgg taatgagtgg
    20101 aatcgacagg cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata
    20161 atcattgggc gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat
    20221 tccagcatga tagagctgca acagcatacg ttcgacgcgg aaaggatgct cgttaatatc
    20281 ttccagcacc agaataccgt tttcgatttg cggcatccac ggtgtaccaa tcagtgaaat
    20341 cagcatcgcg agattgcctc cccataacgt gccttcggtc tgacatgtcg ggccttcccc
    20401 ttgccattca atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa
    20461 ggcgttcagt tcatccgcgc caaagtttgc taccagcatg gggccgctaa aggtgatgac
    20521 attgccatgc gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag
    20581 caacgggtca tgttgttggc gagccaccag tgcctgccag tcaatatccg ccagtaaacg
    20641 actggcaccg taaccaccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc
    20701 aagggaattg agatcctcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg
    20761 ggcaataacc tcgacgttat tgacctgatg ccccgcgtcg gtaaggcgct ggataccacg
    20821 cagcgcatcg tgttgtttta tgcagtaacc cgatggggca attaagtgaa acagagacat
    20881 ggcaattcct tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg
    20941 tcacctcaac ggcgattcca ggctataagg atagaagaag tgaaattaag atggtttgcc
    21001 tttttgattg tgttattagc gggttgttca tcaaagcatg actacacgaa cccgccgtgg
    21061 aacgcgaaag ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt
    21121 gcagcctggg gcgtcgatcc acaattgatc acagcgatta tcgctatcga atcgggtggt
    21181 aatccgaatg cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc
    21241 tccggacgtg atgtttatcg ccgaatgggc tggagtggtg agccgacgac cagcgagctg
    21301 aaaaatccgg agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc
    21361 ccgctggcag gtattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct
    21421 aacggggcag gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa
    21481 atcaacgatc tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg
    21541 caggctccgc gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc
    21601 gcactttgtc cgctttctcc cgggcttccc gctcgagaga gaaaataatc cgctgtaatt
    21661 gccgctccac cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg
    21721 tttcattctt gctatcaatc actttgcgct cgctgattga gattaactgg gcgtcaaagt
    21781 gaaaaacacc ccattgcccc atgttgactt caatctgagc gaagcgcatg ccttcgtgca
    21841 atccggcagg ctttgctgtt tccagtaatg cacccatgcc gcctaacgac aaatcataca
    21901 ggcggaaacg taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga
    21961 gtggggcgga gatgcggaaa tatcggcgtc gttgtacaaa ccacaaggtg ggaggcggta
    22021 cggtaataaa tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaatt
    22081 cgactttcgc gccctgggtt tcggcggtaa tggtaatgtg ctgcgccttt aacacagcgt
    22141 tgttatcttc ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta
    22201 ttttgctgat cagctgcccg ccattccaac tgatacgcaa aggaattgcg gctttgtgca
    22261 aatcgcgtaa cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat
    22321 gactcacgcg gaaaaactcc tgactgacaa actgtctttg gtttatcggt cacagttaac
    22381 aaaacttaat acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacatac
    22441 gcccagcatc gattccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcgt
    22501 ccgattcatc gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg
    22561 atagccggca ttatcgccaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg
    22621 acctgtattc tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc
    22681 attggcggct ccatcagtgg ttttaatctg cacagcttcc tggtggcggt agtgggcgct
    22741 atcctggtgc tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat
    22801 agcgatacag atgccgctat ccgctttcac atcagaatgt atactcgata cctgcccaga
    22861 aattgcgacc ttcttcaata aacccttcac tgtaggcata tttggtatca aacaggttat
    22921 tgaccgacgc attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca
    22981 ccgcaaaacc ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct
    23041 cttccgacag cgttacgctt aacggctccc acggtttgag agtcatccat gcggtcattg
    23101 tctgcgttgg cagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca
    23161 gggcgtagct caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca
    23221 gaccgctgta atccaccgtg ccgctattct gattttgaat ggtatcggca tcgatattgt
    23281 gcgagaggat ggcatcactc acccggttat agtaaacgct gacctcaaac ccccagtcgt
    23341 gcgtgaaggc accattccag gttaaatcca ccccgcgcgc gcgttccggt ttgagctgcg
    23401 ggttaacaat cgctatctgg ttatacgcag gtttggacgt ggtatagcgt tctttcagcg
    23461 tcggaaagcg tgtgcggtca tagtacgaaa gcgccagcgt gtcttcattg gcaaagtggt
    23521 atttccccat cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc
    23581 tgccatcttt ctcatgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattccag
    23641 ccacgacatc gacattatcg gcagcagccc attgatattc actggcgagc gaccaggtac
    23701 gatcttcata gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt
    23761 tccagttaac ggcaaacgac aacagatcgt tttcacgcac atcggctgcc agttgtaacc
    23821 cggcaccgtc gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag
    23881 ccagcgagtt gtacatcatt agcgtatttt caaaggtgtc gcgatacagc cgacttttca
    23941 gggtaaaacg atcgtttagt tgggtcgttc cctgataata aaaactttct ttgtcatact
    24001 ctggccactg ccagtagcgt gatttttgac cactatttcc actgtatggc gggttatctt
    24061 tttcaccatc ctgcttaatg taagtcaatg tgtattcatc gttttcgcgt ggtgtaaaac
    24121 cgagcttcac aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc
    24181 ctgcaatatc attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac
    24241 cgctgacttg caaatacccc agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg
    24301 cattgtcctg gctacggctc catccctggc gatatcccag atttgcttcc agaggttttg
    24361 ttggcttctg ggtggtgata ttaatggctc cgcccatctg attaggcccc tgaagcagcg
    24421 acgaataccc tttggaaact tcgactgccc ccagattgtt ggtcagaatc cgcgccagat
    24481 cgaggttgcc gtcataggga acataaatgg gcacaccgtc gaaatagacc ggcacctgcc
    24541 gactatcaaa gccacgaact tttacctgtt cttcgttgcg gctgcctgac ttttgcagca
    24601 ccacgccggg gacgacactt aatgcctgag cgacattctg tttatccagg gccttcatgg
    24661 tgggttgatc gagaatggtg gtcgtcgtcg atgataccgg actggaccat acggtcagcg
    24721 agtcgctctc ctgagtcgct acggcctgct gggtaaaggc aagtgataat gctgtgcaga
    24781 gataacgttt tttgagtctc atagaataaa ttccgtttaa caaagaatta aatagaggct
    24841 gcgggttgca gactgacttt aatgtcagcc ggtgtcgcgt aataaggcgc ggaggtaata
    24901 aaaaggcgaa tgccgcagtc gaggtaattt ttgagtgttg tcagattaat tccgccggtc
    24961 agagccagcg tgcagtgagg agccagcgac ggtgctattt gcgcaatttc agttgcctgt
    25021 tgcggattaa atttgtcgag ctgaagtacg tctggttgcg cgcgtaacgc ggcgattgcc
    25081 tctttcggcg tgtcggcttc aacaactatt ttcttctcgg gtgcgtggcg acgtaactga
    25141 ttgattgcgc ctgaccagtc ctgattgtca tgaagaaaat ggcggtggtt ggcaaacaat
    25201 aatatggttt ccgcacatcc ggcacgatga atcagtcctc cggcagccag aattgcctgc
    25261 gaggccagta agcgggtgcc cggaattgct tttcgggtgc aggcgatatt gccatcaggg
    25321 taacgttcac gaagtaacgc cagcatttga gcgagataat cagaaacacc gcaactccac
    25381 tccagcacat tctggactgc cttccatccc tgatgaagtg ctgcggcatt accttgcgca
    25441 cggattagcc gctgacccgc gttcgcttgt gaaccgtcgc tgaccgcgtc atcaatagtt
    25501 aatcccagcg tagttaacat cttacacgcg acagaaatgc cgctgacgca gccgccctga
    25561 cggagaaaaa actctatata gccatgctgg tgtccaatat ttaatgctcg cgtggtcagg
    25621 tcaccgccct ggatatcttc cagaagtaac gcatcgattt gtgcctgaga aaggaaaatc
    25681 atcgcagtgc ctcttgcggt acgctatccc accagatgag cgcccagtcg cggctggcag
    25741 gggcgagggc acgagcatct tgttgttgat accagcgata taaccgctca cgctcatcgt
    25801 cgttaagcgc gcccaggctc cagctgacgt tctgctcaaa acgttcccag gtgctgttat
    25861 cctgttggca attttgtcca cggataaaat caacgtgagc ataaattccc atctgataca
    25921 ggacgttgag cgcaaagata tagttgggta attcgatcac ttcccgacca gccgcccgct
    25981 gaatggctgg agagacgaac gaggtgctga ccagatgagt cgtatagacg cgaagccgtg
    26041 cctgattatt cagtttgctc attgcctggc gcatatcagc caccagagtg gagcgggaag
    26101 ccacggcaat atcgcagcgt ggcaggtcac tccagtcctc ttcccaggcg cgctggatcc
    26161 agtggacatt gtctgccttt agcgcggcgg cgcgacgggc ggcaacgttc agcatcccct
    26221 gactgtaatc aacgccataa atcgtcgtga gtttggccgc cagcgccagc gatacggtac
    26281 ccggtccgca gcccatatcg aataaggttt gcgcgccttg caggtcaatt tttgaggtca
    26341 gttgttgcag gtagctgtcc gtaggcgagg cgcaattttc cgccattttt tccgcccgtt
    26401 gatcccagtg agccggcgtt ttttctgtgc ggtgggccag tttgagttgc tgcaggtaaa
    26461 ggtccgcaaa atcaatatca tcaatgagca tgattgttcc ttagtggcta attgcagaga
    26521 ggtgatgatg aatctggtcg gcagaaaccc ggtacagggc agccagtttg ttcgttgtta
    26581 actgttcggt cggtaagcct tgtgttactc gtccatctgg ctctacctgg ataatgctgt
    26641 cggcaatggc gttggcatgg agcggatgat gagtcgacat tagcattgtc atgccgttat
    26701 ttttgagctg cgccagcgtg tccagtagtt ggatctgatg accgaaatcg aggctggagg
    26761 caggctcatc cagtaataac aagcgcggtt gctggacgag tgcacgggcg attaaagcca
    26821 gctgacgttc accgccgcta agcgtattcc agcgtcgtgt cgccagatgc aaaatgccca
    26881 gcttttccag ttgctccgtg gctttcaggc gttcttgctt accgggaacg gaaaaaacgc
    26941 ctatggtggg ggccaggccc atcaacacca tatccagcac actaaaagca aaaatgccgt
    27001 cgtgcgcctg cggcacccag gcaatagcct gcgcgcgctg gcgatggctg agtgtctgca
    27061 cagggaggct atccagtaag atctcgcctt ttaataacgg tataacgcca agaatggagc
    27121 gcatgagtgt ggttttgccg caaccattgg ctcctaacag acaacaaatt gttccttgcg
    27181 acagggtgaa ggacacgtcg cgtagcaccg gatggcggga gtaacccaga gacgcatgac
    27241 ggacggcaag caaagtcaat ggcgacctcc gcgtaacaac aaaaacagga aaaacggtgc
    27301 accaacaaaa gcggtcagaa tgccaagtgg gatttctgtt gttccaatgc tgcgtgccag
    27361 cgtatcggta agcaggagca gaatcgcgcc agtacacatt gccatgggca acagctgctg
    27421 atggttatga ccggtcaaca agcgggcaat gtgcggaacg accaggccga cccaaccaat
    27481 tatgcccgct attgctaccg tgctggcggt gattagtgtg gcgcagacaa ttaatccaaa
    27541 gcgcagacgt gtcacgttca gtcccagcga acgggcttcg tcgtcggaaa gcgtgagtaa
    27601 attcatgcgc cagcggagaa aaaacagggg caaggagcct atgagaatga tcggtgcagc
    27661 atagcagaga tcgcgtaagg tcacggtgga cagccccccc agcaaccaga aggtaattga
    27721 cggtaactgg gtgtaaggat cggccagcgt ttttattagc gatatccccg ctccgcacaa
    27781 cgttcctaac gcaatgccta ccagcaccag ggttaagatc ggatcgtggc gggtcacccg
    27841 acgcgtaatc aaccagacgc ccgcgaccac catcagcccg ccgcaaaatg cgtacagctg
    27901 gatgtaaaca atggataatc cccacaggat tgccgcgcag gcaccaagtc cggcaccggc
    27961 ggagacgcct aaaatatccg gtgacactaa gggattacgg aacattccct gataggtggt
    28021 tccggcccct gccagcgccg cgcccagaaa cagcgcagca agaatacgcg gcacgcggat
    28081 ttgccagaag acaatttgtt cctgcacagg aacattttct tgacgcagaa tgagcgccag
    28141 cagttgctgt atatcgagat gataagcccc acttacggcg gcaaaagtga tactgatagc
    28201 tgccagcagc aaaatacatc cgtttacggt tctcattagc tcgccaccaa tttttgatag
    28261 tcggcgtcac ttaatgggca atgccagaac agttgggcgt aatgctgcat gtcactttta
    28321 aactggcgat tgatggcggg atccagccag gcgtgaagtc tgcgtaatcc cagaagacgg
    28381 ttgattcccg gcggggcatc cagccagcca aagggcaggc cacttaaaaa taagatgcgt
    28441 tgttccgcaa ccgctttcac gccctgccag agaggatcac gacgaataaa atctgcagta
    28501 acggcctcct gaaccagaat aatatccggc tgccagcgca ggagattttc catggaaacc
    28561 tgtgtcagac cgtggcgatc ggctatttgc gcgacgttgt gcaaacctaa taattccgcc
    28621 gcctcggtat gcaacgaacc ctgtaagccc gtttccagcc ctcgaggacc gcgcgcagca
    28681 taaaagctga ggttagcggc gggtgaggtg gcgaatgcct gagcttcacc aacgaagcgg
    28741 ctggcgagat tggcttgttc tgcggtgcgg tgctcttcgc ctaacgtttt gccaagcgtt
    28801 gttaactgtt ctgctgattg ttccagtttc ccgttaagca ataaccaggg tatttgtgtc
    28861 tgttcgctaa cctgccgtgc cagggagatc caggtttcat ccgtattgcc gcaatcaacg
    28921 accaaatcgg gatgtaacgc cataagccct tccagcgaga gtgtgctggc gcgcccggcg
    28981 agtcgtccca gcctggggaa ctggcgaatg tgctctggca atggaattaa tgcctgacgg
    29041 gcaaaatcaa aagaggaaaa accgaccatt ttttctggcg cgacagccag caatagtaaa
    29101 tcggcgggcg cgcctgcgct gacgatgcgt gtaatgtttt gggcttcggg aagcgtcagt
    29161 gaggcccaga atttgactga ttgtgcaaag gtcggcagat gatgcaggaa caatgtcgac
    29221 gcaagaatct gtgtaaacct ccgtctggtt agtgacataa gttatatcct gataataaaa
    29281 accgctatat atttacatat atatcgaaaa aaatcatgtt tgatgtcaaa atttgtctaa
    29341 agagacagaa tgatttgctt gttgcttaaa taacactttg ttccacaaga aaattgtcag
    29401 ggagaatggc ggagtggcga aaaatataaa tagaggtgta atgattaaca aattgataaa
    29461 ataaaattaa aatcttctct gataaaagaa aataaaggca tagaccgtag aatggggggc
    29521 atttcgtggc gaaaaaagag caatgccaat tttatgcaca taatggcatt gctcgatata
    29581 cagcaaagtt aaggcgtagg ttccgcctct ttttgtttta ccggctgtgt tgttgattca
    29641 ctgagtggac gcgtcgccgg aacattgtca cacggttgct ctttcgggca gatcaaatcc
    29701 agcattttca gcgtcacgcc attggtccag ccaaagccat cctgtaatgg atattcgcca
    29761 ccgccgcccc ctgttccggt ggtgctgaca tcatatttct cgaccagctt cttctcccgg
    29821 tcataagtgt gctgaacatt ggtcaggaag tgccagctaa tgtccatcgc cacctcgttc
    29881 tgcccgtagt tttgcaaccc ttctgtcgcc acccattgca ggggagccca gccatttggc
    29941 gcatcccatt gctgtccact tttcaccgac gtggtgttca ggccgcctgg ttgcagcaga
    30001 tgtgttttcg tcgccgtcgc cattttgttg gcgcgatctt tcgctgccgc attgacgtac
    30061 agcgggaaca gggcggcagc ggttaactga ttgcgcactt tatgactttt caggtcgtaa
    30121 tcggcatacc agccttgttg atcgttccac aggtattttt cgatcccttt ttgacgggca
    30181 ttcgccagcg tttcgtactg gtttgccatc gcgttatctc cggaagcttt gctggcgcgg
    30241 gcgaggattt tttccatttt aaacatcagg ctgttcagat cgaccggtac gatgctggtg
    30301 gtgcgtaagg tatttaactg ctgcggattg tccatccagc gcgagctgaa atcccagcca
    30361 gacgcagcgg cagagcgcag gtcgcggtaa atttcagtgg caggtcgatt cggattgctt
    30421 ttggcggtgg caatatcttc cacccatgac tctggtcgtg gcgtatcgcg atcgtcccag
    30481 tagcggttga gaagggtacc atcctgaagt tttacaacgc gtttttcctg ttgtccggct
    30541 tgcaggtttt caacaccgtc catccagtaa gcatattctt tttgcatttg cggcaggtat
    30601 tgcttcaacg cggcatcgcc ttcatgctgc gccagtaact ctaccatcag ggcaaagaag
    30661 ggcggctgcg agcgacttaa atagtaactg cggttgccgt tgggaatatg accgtaagtg
    30721 tctatttcat gagcaaaatt ggccaccata tccgcgactt tatcccagtg gccgctttcg
    30781 gcaagcccca gcatggtgaa gtaactgtcc cagtaataca cttcgcgaaa acgtcctccc
    30841 ggcacgacat agggttccgg taacggcaac agagaatccc atttctcggt actttcggta
    30901 gaacgggtta acaccggcca aagtccgtct atatgttcgc gcagtgactg cccctttgga
    30961 ggcacatatt tctctccctc tttcggcagg gtgaaattga cgttaacgaa atggcgcaga
    31021 tcaaatccgc tctggttttg ctgcatccga taatcagcaa ggatcatcag cggatcgctg
    31081 ttcggcacgg catctgcaaa ggttttttgg tccggaaaaa gtttggcgtt ttgcacatca
    31141 ttaaacagcg gccctaataa aatatcaggc ggctgtggcg ttaccggtgt ttcttctgcc
    31201 tgcaccgata gcgcagcgaa acacaaaaag atgcaggctg gaattaacgc catttttagc
    31261 gggcgagaag ctgcggggga tttcatcaat cattctcctt tggcgaaacc gaataaaacg
    31321 gtattcaagt atcagaaaac cttagttcag gatcgcacta gctgcatgat cgaaatctca
    31381 ttctgcgaac ggtcaggcat tttagaaaag ggaattaaag ggataaataa cgtcgaagta
    31441 gagtgatcaa ataacgtctg tttgttagcc agccagcgga gttgggttcg cgattagcgc
    31501 agaaaaaatc atcatgattt ggtaaaaaat ataacaggta gttaaaaatc atccgtgc
//
LOCUS       DAPOZO010000059        30211 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPOZO010000059 DAPOZO010000000
VERSION     DAPOZO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.059"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(40..1902)
                     /gene="kefC"
                     /locus_tag="ROI21_002859"
     CDS             complement(40..1902)
                     /gene="kefC"
                     /locus_tag="ROI21_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDX5111760.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSSEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(1895..2425)
                     /gene="kefF"
                     /locus_tag="ROI21_002860"
     CDS             complement(1895..2425)
                     /gene="kefF"
                     /locus_tag="ROI21_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDX5111761.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVLQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHDKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(2533..3864)
                     /locus_tag="ROI21_002861"
     CDS             complement(2533..3864)
                     /locus_tag="ROI21_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5111762.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQAQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(3923..4210)
                     /gene="fixX"
                     /locus_tag="ROI21_002862"
     CDS             complement(3923..4210)
                     /gene="fixX"
                     /locus_tag="ROI21_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDX5111763.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(4207..5493)
                     /locus_tag="ROI21_002863"
     CDS             complement(4207..5493)
                     /locus_tag="ROI21_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDX5111764.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLARAGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEVDGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSVPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(5544..6485)
                     /locus_tag="ROI21_002864"
     CDS             complement(5544..6485)
                     /locus_tag="ROI21_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDX5111765.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(6500..7270)
                     /locus_tag="ROI21_002865"
     CDS             complement(6500..7270)
                     /locus_tag="ROI21_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDX5111766.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            7743..9257
                     /gene="caiT"
                     /locus_tag="ROI21_002866"
     CDS             7743..9257
                     /gene="caiT"
                     /locus_tag="ROI21_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDX5111767.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            9288..10430
                     /gene="caiA"
                     /locus_tag="ROI21_002867"
     CDS             9288..10430
                     /gene="caiA"
                     /locus_tag="ROI21_002867"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDX5111768.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            10548..11765
                     /gene="caiB"
                     /locus_tag="ROI21_002868"
     CDS             10548..11765
                     /gene="caiB"
                     /locus_tag="ROI21_002868"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDX5111769.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTSEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGKIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            11839..13392
                     /gene="caiC"
                     /locus_tag="ROI21_002869"
     CDS             11839..13392
                     /gene="caiC"
                     /locus_tag="ROI21_002869"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDX5111770.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIAAHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            13501..14286
                     /gene="caiD"
                     /locus_tag="ROI21_002870"
     CDS             13501..14286
                     /gene="caiD"
                     /locus_tag="ROI21_002870"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDX5111771.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     IEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            14292..14882
                     /gene="caiE"
                     /locus_tag="ROI21_002871"
     CDS             14292..14882
                     /gene="caiE"
                     /locus_tag="ROI21_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDX5111772.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVEAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLIGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(15001..15396)
                     /gene="caiF"
                     /locus_tag="ROI21_002872"
     CDS             complement(15001..15396)
                     /gene="caiF"
                     /locus_tag="ROI21_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDX5111773.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(15657..18878)
                     /gene="carB"
                     /locus_tag="ROI21_002873"
     CDS             complement(15657..18878)
                     /gene="carB"
                     /locus_tag="ROI21_002873"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDX5111774.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(18896..20044)
                     /gene="carA"
                     /locus_tag="ROI21_002874"
     CDS             complement(18896..20044)
                     /gene="carA"
                     /locus_tag="ROI21_002874"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDX5111775.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDTDEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(20500..21321)
                     /gene="dapB"
                     /locus_tag="ROI21_002875"
     CDS             complement(20500..21321)
                     /gene="dapB"
                     /locus_tag="ROI21_002875"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDX5111776.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRGGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(21482..21847)
                     /locus_tag="ROI21_002876"
     CDS             complement(21482..21847)
                     /locus_tag="ROI21_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDX5111777.1"
                     /translation="MTYGDAYLAGWKNIFNYNGVATRFEFWSFIIGSAIICLLPLLSW
                     LFAITIDDDYGAFIFFALPTSFILTLIFAVPGMALGFRRMHDIGRSGWWFSIGVLIPY
                     AGVILFILCCLPSKPQEQA"
     gene            complement(21869..22783)
                     /gene="rihC"
                     /locus_tag="ROI21_002877"
     CDS             complement(21869..22783)
                     /gene="rihC"
                     /locus_tag="ROI21_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDX5111778.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRNAASVHGESGMAGYDFVEHNRKPLEIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPHIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPSNVQVALDLDVRGFQQWVAEVLALAL"
     gene            complement(22849..23799)
                     /gene="ispH"
                     /locus_tag="ROI21_002878"
                     /gene_synonym="lytB"
     CDS             complement(22849..23799)
                     /gene="ispH"
                     /locus_tag="ROI21_002878"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDX5111779.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(23801..24250)
                     /gene="fkpB"
                     /locus_tag="ROI21_002879"
     CDS             complement(23801..24250)
                     /gene="fkpB"
                     /locus_tag="ROI21_002879"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDX5111780.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTIHFDIEVLEIDPALEA"
     gene            complement(24338..24832)
                     /gene="lspA"
                     /locus_tag="ROI21_002880"
     CDS             complement(24338..24832)
                     /gene="lspA"
                     /locus_tag="ROI21_002880"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDX5111781.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(24832..27648)
                     /gene="ileS"
                     /locus_tag="ROI21_002881"
     CDS             complement(24832..27648)
                     /gene="ileS"
                     /locus_tag="ROI21_002881"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDX5111782.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALIAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLAGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLFAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(27691..28632)
                     /gene="ribF"
                     /locus_tag="ROI21_002882"
     CDS             complement(27691..28632)
                     /gene="ribF"
                     /locus_tag="ROI21_002882"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDX5111783.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(28640..28858)
                     /locus_tag="ROI21_002883"
     CDS             complement(28640..28858)
                     /locus_tag="ROI21_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDX5111784.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            28961..29224
                     /gene="rpsT"
                     /locus_tag="ROI21_002884"
     CDS             28961..29224
                     /gene="rpsT"
                     /locus_tag="ROI21_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDX5111785.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            29708..>30211
                     /gene="espX1"
                     /locus_tag="ROI21_002885"
     CDS             29708..>30211
                     /gene="espX1"
                     /locus_tag="ROI21_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HDX5111786.1"
                     /translation="MTDGISTSPHCLYKSNIADDVIINKTRQNELVKVFCEYKTEFLI
                     LFDDFFRSQDLPKPSPVLHHFFQYTHLRDAHFYRCKLIEHTVQFSFFKHKEITLLRLD
                     VFDDRTSECLSEEIKTYQECYEKFIKFLKANFNQEIYPQLYIPEIFYDACKHLQSFFY
                     HQEKSQNA"
BASE COUNT         7376 a         8220 c         7559 g         7056 t
ORIGIN      
        1 aaacagttag ctgtaaagtg tgatttacgt cactctttat taggatgagg gtttcgtttc
       61 cggttcatcc gccatgttgc cggtatgttt accttcttcg gttccctgcc agccatgtcg
      121 ttgaattaat gacagatgtt cgcggtcctc ggtaatgatc tcacttaaca tcgcgctagt
      181 gcgtttatag accgccgcgc gggctttggt gtcgttctca accattgcca tctcttccac
      241 catctgaata ttaaagcggc ggaacacatc ggcacgttct cgcgcttcat acggccccag
      301 acctaaactt tccagtgcca gacgcccggt tttcagcgca ccttcgaagg tttcacgctc
      361 cggcttttca acgcctgcct gacgcaaacg aatgtagtgg tcgacatcgc gggcgcgggc
      421 aataatctgc aaatgcggga aatgttcttt caccatctcc gtcagttgca ggttggtttg
      481 cggatcgtcg atggcgttaa tcagcacttc cgctttcgcc gctcccgcag attccagtaa
      541 atccatccgc gtggcatcgc cgtaaaacac tttcatacca aatttacgca aggtttcgat
      601 atggtccgga tcgtgatcga gcaccaccat tttcaccccg ctggagagca gtaaacgtcc
      661 ggtgatctgc ccaaaacgac cgaatccggc gatgattacc cgcggctgtt cttcgtcgat
      721 ctcatcggct tcacgcgcct cttcactaga agattgctca aggcgattga ggatcaccag
      781 cagaatcggc gttgccgcca tcgacagcgc caccgccagg gtcagcgatt tcgcccactc
      841 cggctccagc acattcgcca tctgcgccgc accaaatacc acaaaggcaa actcactacc
      901 ctgccctaac aacaccgcaa accagcgacg ctgtttattt ggcacctgca acggtcgggc
      961 aatcagccac aacatggcga ttttgatgat gaggaaaccg agcagcaaaa tgacaatgcg
     1021 caatgggttt tcaatcagcg tgccaaagtc tatcgacatg ccaacgccaa tgaaaaacag
     1081 ccccagcagc aaacctttaa agggttcgat atcgctttcc agcgcatgac gatattccga
     1141 gcttgccagc agtacgcccg ccagaaacgc gcccatcgcc atcgacaagc caacctcttc
     1201 cagcagcaaa ccaaagccaa acacgaggaa taacgccacg gcactaaaca cttcccgcaa
     1261 gccagagcgg gcaacaaaac gcagcgccgg acgcgtgaca tagcgcccca gcaataccac
     1321 cagcaccagc gcacccgcca cttttaacgc cgagagagca aatgcgccca tcgtcgtcga
     1381 ggcactgctc gctgccagta gcggaatcat cgccaccagc gggatcgccg cgatatcctg
     1441 gaacagcagc accgcaaagg cactgcggcc catttgcgtc accatcagat tgcgttcatt
     1501 catcgcctgc atggcaatcg ccgttgatga gagcgccagc gtcatgccga tcaattccgc
     1561 gacctgccag cgcagcccaa gcaacataca gaacagcccc agcaggccgc cgcaaatcac
     1621 catctgcaat gcgccaccac cgaacaccgc cgcacgcagc ttccacagcc tttgtggatc
     1681 gagttcgagg ccgataataa acagcatcag caccacgcca atctcggcaa agtgcagaat
     1741 agattcggca tcggtcacca gtcgcagccc ccacgggcca ataatgcagc cagcgatcag
     1801 gtagccaagt accgatccca gaccaaggcg caccgcaatg ggtacaatca gcgccgccga
     1861 accgagataa atcagcgcct gaatcaaggt atgactatcc atgatgtgcc tcctgccatt
     1921 ccagcagacg ttgcttatag tggcgcgcct gcccttcgag ggtttcgtcg tcacaaataa
     1981 aggtgcagtg catggcaaac ggtggcagcc agttcagccc gcagtagatt gccgtcgcct
     2041 gtagcggctg tgacagcaca tcaaagcccg gatgcgcacc aatctcaaaa tggctttccc
     2101 caccgccggt cgtcaccgcc cacagtaaat gtttgtcatg cagcgccgtg ccgccgtgac
     2161 cgtaagccca gccgtgcgag aagactttat cgatccaaag tttaagaagc ggaggaatgc
     2221 tgtaccactg catcgggtgc tgcaagacga tcagatcggc gcgagacagc gcctcctgct
     2281 cggcggcaat atcgatattg aagtcaggat agagttgata aagagagcga atttcgacgc
     2341 cttccagcgt ccttgcctgt tcaagcatcc gtttattcgc atgggaatga tgcggatatg
     2401 gatgcgcata aattataaga atcataggta gcccgcgtga ttacgccttt gttttcccac
     2461 aaagtgtagt catatattca cgcagcttat agtcaataat attgattcag aaaagagaga
     2521 aatctgaaca aattacccca ttctctgccc gcggatcgtc atattgctgg tctgcgccag
     2581 tgacatccct cgagtctccg gggcaaacgc tacggaaatc aacaagccaa acagcgagat
     2641 acccgccccc attagcatcg tgttactgat accgtaatta ttgataaaga tcggcagtgc
     2701 ccaggtcgaa acaatagtgc caatacggct taaggacata atcacgccca cggcagaggc
     2761 gcggatatcc gtcgggaaga gctcattagg atagagccac tgcaaattac ccggcccgcc
     2821 agagaaaaag gcatataccg caaaggccat cactaccagc cagatcccca tatccgggat
     2881 cagccccaaa accgccagcg ccagcgtcat catggcaaag ctgccaatca acaatggacg
     2941 ccgtccggca gtgtttaacc acagcatcgg cggaatacag ccgagcataa agaacaggct
     3001 aatcaccacg ttccccagtg ccgcgttttt gccaacaccc aatcccaaca aaccaacgat
     3061 ttgcgggcca aaggtgtaaa tggcgaacat tgggatcacc tggcaggtcc agatggcggc
     3121 aacaaacaga acaaaaggaa aatggcggcg attaaacaga tcgcgaaaac gggtttgctg
     3181 cgcctgctct tcatcgaaag ccaccggttc gccaaacagt ttgatcatca tctcttcgca
     3241 ctcttttact cgccctttgc gtaacaacca gcggggagat tcaggcagtt cgaatcgacc
     3301 aatcaaaatc aacaaacagg ggatcgctgc gctacccagc atccagcgcc aaccaccttc
     3361 cacatcataa agccagtaac cgaccagatc ggcgcaggtc gcgccgacat accacatggc
     3421 ggcgataaag ctgatggaaa atgcccgctg acgggtactg gagaactcag tgatcatcga
     3481 agtggcgatg ggataatctg caccgatgac aatgccgatg agtacccgca tcactaacag
     3541 ttcgacgggg gatgagacaa acatcgtcgc caccgatatc acgccgatgg cgatgatatc
     3601 gatgaggaac attttgcgcc gtccgacttt atcagagata tagccaaaca gcgatgtgcc
     3661 aacgaacagc ccggcgagcg ttcccgcgcc cagcaagcca atccagtcag cgtccagttt
     3721 cagcgccgga gtcagttgct ccagcgccac gccaatcatt accagtacat aaccatccag
     3781 aaacggacca ccgcttcccc acagcaaaat gcggcggtga atagaagaga atttaagatc
     3841 gtcaaagttt ctggacggtt gcatgggtta ttcctgtttt tttgcaatga gagtcagtac
     3901 ggcgttacgt atcaaaccaa catcagccgt aacggaactc cacaccaaag gtgccgcgcg
     3961 ggtattccca ctgttccagc gccgacccca gccccagaat gcgacaggtg ccgcactcca
     4021 gacatccggc gtaatcgaaa cgcacgcttc cgtcatcctg cttcttgtac agacctgcgg
     4081 ggcacgcttt caccagcagc tccagcacct gtttatcagc atcggccttc acaacaatgt
     4141 gcggatgctc ttcatcgaca ttgaatttat tgacgcccag tttgacgtcc acattgacgg
     4201 gagaagtcat aaaacggtca ctcctttcat gccatccttg attagattga tgaagcccac
     4261 tttcttaccg tggcggagga ttttcttgcg catcagttcc ggcacactgc cgtcaatggt
     4321 gaacaggtca cgcgccacgc ccaccgccag ttccgggtag ccgctaaaca tgcgtgggtt
     4381 atcaaggaac gccggtagtt tctgatacat acgcatatcg cgcagcggac cactctcaag
     4441 atgctgacga tattccgcca gtttttgctt actgaaatcg tcgcttttca tcgctgaaag
     4501 cacggttttt gctgcggctt ccccggcggc aatcgccaga tccataccgc gaatggtaaa
     4561 accgaggttc atacacattc cggcggcatc accggcaatc agtacgccgt caccgaccag
     4621 ctccggcagc atgttgatgc ctgcttccgg cactacgtga gcggaatatt ccaccagctt
     4681 gccgcctgcg atcagcggtg caacggccgg atgttgtttg aaatcttcca gcatttgcgg
     4741 caccgatttt ttcgcgtcat gtagatgatg gagaccacaa accaacccca gcgacagagt
     4801 gttttcattg gtataaagaa agccgccgcc catcaggcca tcggtggggg aacccgcaaa
     4861 caggcaagcc gcaccctgat taccctgcaa ctgaaaacgg tcttcgataa ccgacttcgg
     4921 taactcgatc agttccttca cgccaaccgc cacatccgtc ggtttgacgc gttttgccat
     4981 ccccaacttt tcggcaagga tggagttcac cccatcagca aggatcaccg ttttcgcttc
     5041 aatcacatcg ccatcgactt ctacaccgac aactttgcca tcgcgctgta cgaggttatc
     5101 gacgcggatc ccggtaatta actgtgcgcc cgcttcttcg gcctgctcca tcaaccaggc
     5161 atcaaattta ctgcgcaaaa cggagtaaga acgctgggat ggcgaggttt cgtcaccgtt
     5221 gcagtagtcc atagtcatcg ctgacttttc cgtcataaac gcgagttttt catgggtgat
     5281 caggcgttct acgggggcgg attcggcgaa acctggaata atgtgttcca gactgtgggc
     5341 atagagacgc ccgccagtaa cgttcttggc acctgcggaa ttgccacgct cgataactaa
     5401 cacttgcgcc cctgcgcggg cgagcaccag tgcggcaacc gaaccggcaa gcccagcacc
     5461 gacgatgatg gcgtcaaaga tatcttcgga cataagagct ccctgtcagc ggcagggcca
     5521 aaatgcagcc ctgccagagt ggatcaacgc gctaaagctg cggtcagtgc cggaaggatc
     5581 ttcacggcgt cgccaacaat gccgtaatcc gcgtactgga agatcggcgc atttttatct
     5641 ttgttgatgg cgaaaatggt ttgcgacgcg ttagcgccaa ccatgtgctg gatctgcccg
     5701 gagatcccca ccgccaggta cagttcaggt ttcagcatca ggttggagat accgacatag
     5761 cgttcgtgct ccatccattt ttcgttttct gccaccggac gagaacaggc caactccgca
     5821 cctatcgcct tacaaagctg ttctgccagc gcaatgttct ctttgctgcc aatgccgcga
     5881 ccgacgctga ccaccagacg ggctttgtcg agatcgacgc tgttgctctg gcgcgcctgg
     5941 gttgccgtgc gggtaatcgc cacagccgga gcctgccact ccacggtgtg cgtttcgcca
     6001 gtgcgtgacg cgtctggctg agccgcatca aacgtgccgc tgctgatggt cagtaccgca
     6061 tacggcgtgg caatgcgttc ttcgccaatc gccagaccgc cgtaaaccat gtgtttcact
     6121 gtcgctttac cgtcctgtac gctgacggtg ctggcatcgt tagacaccgc cgctttaagg
     6181 cgataaccca gttttgccgc cagtaatttg ccgcgacggg tgtttggcag cagcaccaga
     6241 ccgtctgcgc cgtgctggcg aatagtgtca gccatgacac cggcgtaatc ttcgatcatc
     6301 cgatcgtccg gtttgccgtt taatttccag acatgattag cgcctagctg gattgcctgt
     6361 gcgccgtcgg catcattgag gacaaaggca ttgatttgat tagctaaagc ctgcgcaccg
     6421 ttcatcagtt ccggcagacg agaaggggta tcgctgaata cccagacttg agaaaacgtg
     6481 ttcatagcat cccctgtaat taaatgactt tgcgaagatt ttcagcaaat gcggcgatct
     6541 gttcttcgcc gtcgccttca atcacgatgc gctgccgctc gcgctgtttc ggcgcggcaa
     6601 cctgttgttc tgaccaggct gcctctgcgt taaaaccgat atccgccgcc gaccatacct
     6661 ggactggctt tttcgccgcg ccgagaatgg ctttcatcga aggaatttgt ggggagttga
     6721 tatcagtgga aacagcaaca accgcaggca gcggaatgct taaggtttct gtttcatctt
     6781 ccagttcgcg ctcaacggtg agggtatctg ccgtcaggga gataattttg ctgacgccgt
     6841 taactgccgg aatattgagg atttcgccta ccagcaaacc aacctgctgg gcataaaggt
     6901 cggaagaacc atcgccacag aggatcaaat caaagcctgc tttctgggcg gcggcagcca
     6961 gtgcgctcgc cgtttgttgc ggcagtgcct gctcgaactg gtcatcaatc accacaatca
     7021 gttcatccgg accgcgcgat agcacatctt tacgcccttt ggcgttggtc agggctttac
     7081 cgcccacact taaggccgtc acctgcgcct ctgctgcctg ttgctttagc tgacaagccg
     7141 cttcaatagc gttgagatcg tattggctta ttttggcatc ggctttactg aagtctaacg
     7201 aaccatcagc attattgacc gcaatatcct gttcatcagg cacgcactta tagcaagtaa
     7261 taatcttcat tgcatctcca gaaatcatga aggaaacgtc gttcatatcg gtggtgttaa
     7321 ttacagatat cacgcccttt aatttggaaa taaaacttta atcaccaata ttgaaaatgt
     7381 cacacgcaga taaatccaac tttcaatatt gttaagtccc tcaccaatat tgaaaacatg
     7441 gcgtagcaaa aagaaatttt caatattgct ttatggatca ccaatattga aagtcagatg
     7501 ctattttttt actcacaatc aaactcatca cattgataaa caatgaatat ttacacaaag
     7561 acgccctgtt tttacccgat aaaaaactgt gactaacatc acaaaacgca gccaattgaa
     7621 tacccattat gagttagcca ttaacgcgtc cacgaggtta ataataatta tattaaatgt
     7681 taacaaaaat aaaacaaacg ggaacgcaaa ataaatattc gttttcacag tggaattaac
     7741 tcatgaagaa tgaaaagaga aaaacgggaa tagaaccgaa ggttttcttt ccgccgttaa
     7801 taatcgtcgg catactttgt tggcttacag taagagatct ggatgcggcg aatgtcgtta
     7861 ttaatgctgt attcagttac gtcaccaatg tatggggatg ggcatttgaa tggtatatgg
     7921 tggtgatgct tttcggttgg ttctggctgg tgtttggccc gtatgccaaa aagcgtttag
     7981 gtaacgaacc accagaattt agtaccgcca gttggatctt tatgatgttc gcctcctgta
     8041 cgtctgctgc cgtactgttc tggggatcga ttgagatcta ctactacatc tccaccccgc
     8101 cgtttggcct agaaccgaac tcgacaggag cgaaagagtt ggggctggct tacagcttgt
     8161 tccactgggg acctctgccg tgggccactt acagcttcct ttcagtcgcc ttcgcttact
     8221 tcttctttgt ccgcaaaatg gaagtgattc gccccagctc cacactggtg ccgctggtag
     8281 gtgaaaaaca cgccaaaggg ttgttcggta ccatcgtcga caacttctat ctcgtcgcct
     8341 tgatcttcgc gatgggtacc agtctgggcc ttgccacgcc gctggtgacc gagtgtatgc
     8401 aatggttgtt tggcattccg cataccctgc aactggacgc tatcatcatt acctgctgga
     8461 ttatcctcaa cgccatttgc gtcgcctgcg gtctgcaaaa aggggtacgt atcgccagtg
     8521 acgtgcgtag ttacctgagc ttcctgatgc tgggttgggt gttcattgtc agcggtgcca
     8581 gcttcatcat gaactacttc accgattcgg tggggatgtt gctgatgtat ctgccgcgca
     8641 tgttgttcta taccgatccc atcgctaaag gcggcttccc gcaggggtgg accgtgttct
     8701 actgggcatg gtgggtgatt tacgctatcc agatgagtat cttcctcgcc cgcatctccc
     8761 gtggtcgtac cgtgcgtgaa ctgtgcttcg gcatggtgct ggggctgaca gcgtcaacct
     8821 ggatcctgtg gactgtgctc ggtagtaaca ctctgctgtt gatggataag aacatcatca
     8881 acattccaaa tctgatcgaa cagtacggtg tggcgcgcgc catcatcgaa acctgggccg
     8941 ctctgccact cagcaccgcc accatgtggg gcttcttcat cctctgcttt attgccaccg
     9001 ttacgctggt taacgcctgc tcttataccc tggcgatgtc cacttgccgc gaagtacgcg
     9061 atggtgaaga accaccgctg ctggtgcgta tcggctggtc aattctggtt ggcattatcg
     9121 gtattgttct gctggcgctc ggcggcctga aaccgattca aaccgccatt atcgccggag
     9181 gatgcccgct gttcttcgtc aacattatgg tgacgctctc ctttattaaa gacgcgaaac
     9241 agaactggaa agattaatta acccccaaaa tatcaagagg ttgaaagatg gattttaatt
     9301 taaatgatga gcaggaactg tttgtcgccg gtatccgcga actgatggcc agcgaaaact
     9361 gggaggccta ttttgccgag tgcgaccgtg acagcgtcta cccggaacgt tttgtcaaag
     9421 cactggcgga tatgggtatc gacagtctgc tgatccctga agagcacggt ggtctggacg
     9481 cggggtttgt tactctcgcc gccgtgtgga tggagctggg acgtctgggg gcaccaacct
     9541 atgtgctgta ccagttgccg ggcgggttca ataccttcct gcgcgaaggc acacaagagc
     9601 agatcgacaa gattatggct ttccgcggca ccggtaagca gatgtggaac tcagcgatta
     9661 ccgagccggg cgcgggctcc gacgtgggta gcctgaaaac gacttatacc cgtagaaatg
     9721 gtaagattta tcttaatggt agtaagtgtt ttattaccag cagcgcctac accccgtaca
     9781 tcgtggtgat ggcgcgcgac ggggcttctc cggacaaacc tgtctacacc gaatggtttg
     9841 ttgatatgag caaaccgggc atcaaagtga ccaaacttga aaagctcggt ctgcgtatgg
     9901 atagctgctg tgaaatcact tttgacgacg tggaactgga cgagaaagac atgttcggtc
     9961 gggaaggtaa cggctttaac cgcgtcaaag aagagttcga ccatgaacgt ttcctggtag
    10021 ccctgaccaa ctatggtacg gcgatgtgcg cctttgaaga tgcggcgcgc tacgccaacc
    10081 agcgcgtgca gtttggcgag gcgattggtc gtttccagtt gattcaggaa aaattcgccc
    10141 acatggcgat caaattaaac tccatgaaaa acatgctgta tgaagcagcg tggaaagcag
    10201 acaacggcac catcacctct ggcgatgcgg caatgtgcaa atacttctgc gccaatgcgg
    10261 catttgaagt ggtggatagc gcaatgcagg tgctgggcgg tgtcgggatt gcgggcaacc
    10321 accgcatcag ccgcttctgg cgtgacctgc gtgtagaccg cgtctccggg ggatctgacg
    10381 aaatgcagat cctgacgctg ggtcgcgcgg tgctgaagca ataccgctaa gtccgtgcgc
    10441 tgcctgatgc gacgctgacg cgtcttatca ggcctacggt cggccggata aggcgtttac
    10501 gccacatccg gcagtcatgc gctacataaa tttttcagga gctaataatg gatcatctac
    10561 ccatgccgaa attcgggccg ctggccggat tgcgcgttgt cttctccggt atcgaaatcg
    10621 ccggaccgtt tgccgggcaa atgttcgcag aatggggggc ggaagttatt tggatcgaga
    10681 acgtcgcctg ggccgacacc attcgcgttc aacctaacta cccgcagctc tcccgccgca
    10741 atttgcacgc gctgtcgtta aatatcttca aagatgaagg ccgcgaagcg tttctgaaat
    10801 taatggaaac caccgatatc ttcatcgaag ccagtaaagg tccggccttt gcccgtcgtg
    10861 gcattaccga tgaagtcctg tggcagcaca acccgaaact ggttatcgct cacctgtccg
    10921 gttttggtca gtacggcacc gaggagtaca ccaatcttcc ggcgtataac accatcgccc
    10981 aggccttcag tggttacctg attcagaacg gtgatgttga ccagccaatg cctgccttcc
    11041 cgtataccgc cgattacttt tctggcctga ccgccaccac ggcggcgctg gcagcattgc
    11101 ataaagtgcg tgaaaccggt aaaggcgaaa gtatcgacat cgccatgtat gaagtgatgc
    11161 tgcgtatggg ccagtacttc atgatggatt acttcaacgg cggcgaaatg tgcccgcgca
    11221 tgaccaaagg taaagatccc tactacgccg gttgcggtct gtacaaatgc gccgacggct
    11281 acatcgtgat ggaactggtg ggcattaccc aaattgaaga gtgctttaaa gatattggcc
    11341 tcgcacatct gctcggtacg tcggaaatcc cggaaggcac tcagcttatc caccgtatcg
    11401 aatgccctta cggcccactg gtagaagaga aactcgatgc ctggctggct gcacatacca
    11461 tcgcagaagt taaagaacgc tttgccgaac tgaatatcgc ctgtgccaaa gtactgaccg
    11521 taccggaact ggaaagcaat ccacagtatg tggctcgcga atcaatcact cagtggcaaa
    11581 cgatggatgg tcgcacctgc aaggggccga atatcatgcc gaaattcaaa aataaccccg
    11641 gaaaaatctg gcgcggaatg ccctcacatg gcatggacac ggctgccatt ttgaaaaata
    11701 tcggctacag cgaaaacgac attcaggagt tggtcagcaa aggtctggcc aaagttgagg
    11761 actaaacgcg tggcgtcagt cggcttgcct gactagcgcc cgcccaataa caaacgaaca
    11821 acaatggata gaggtgcgat ggatatcatt ggcggacaac atctacgtca aatgtgggac
    11881 gatctggcgg acgtttacgg tcataaaacg gcgctgattt gtgaatccag cagcggagtc
    11941 gttaaccggt atagttatct tgagttaaat caggagatta accgcacggc aaacctgttt
    12001 tatacgctgg ggattcgcaa aggcgacaag gttgctctac atctcgacaa ctgcccggaa
    12061 tttatctttt gctggttcgg gttggcaaaa attggcgcga ttatggtgcc gattaacgcc
    12121 cgcctgttgc gcgaagaaag cacgtggatc ctgcaaaata gccaggcatg cctgctggtg
    12181 accagtgcac aattttatcc tatgtatcaa cagattcagc aggaagatgc cactcagttg
    12241 cggcacattt gcctgacaga tgtggcactt cccgctgatg atggcgtgag ttcgtttact
    12301 caactgaaaa atcaacaacc tgccaccttg tgctatgcac cgccgctatc gactgacgat
    12361 acggcggaaa ttctcttcac ctccggcacc acctcccgac cgaaaggggt ggtgattacc
    12421 cattacaacc tgcgcttcgc tggatattac tccgcctggc aatgtgcact gcgtgacgat
    12481 gacgtctacc tgacggtaat gcctgcgttt catatcgatt gccagtgtac tgcggcgatg
    12541 gcggcgtttt ctgccggggc cacctttgtg ctggtcgaga aatacagcgc ccgcgccttc
    12601 tggggacagg tgcagaagta ccgtgccacc attaccgaat gtattccgat gatgattcgt
    12661 acgttgatgg tgcagccgcc ttcagcgaac gatcggcaac accgcctgcg ggaagtgatg
    12721 ttttatctca acctgtcgga gcaggaaaaa gacgcgtttt gtgaacgctt cggcgttcgc
    12781 ttgctgacgt cttatgggat gacggaaacc attgtgggca ttatcggcga tcgccctggc
    12841 gataaacgac gctggccgtc gattggtcgg gcggggtttt gctacgaagc ggagatccgc
    12901 gacgatcaca atcgcccgct cccggcaggt gagatcggtg aaatctgtat taaaggcgta
    12961 ccagggaaaa ccatcttcaa agagtatttt ctcaacccga aagccactgc gaaagtgctg
    13021 gaagccgatg gctggctaca taccggcgat accggatacc gcgacgaaga gggctttttt
    13081 tatttcgtcg atcgccgctg caatatgatc aaacgcggtg gcgagaatgt ctcctgcgtg
    13141 gagctggaaa atattatcgc cgcgcacccg aaaattcagg acatcgtggt tgtgggtatt
    13201 aaagattcga ttcgcgatga agccatcaaa gcatttgtgg tgctgaatga aggtgaaaca
    13261 ttgagcgaag aggaattttt ccgcttctgc gaacaaaata tggcgaaatt taaagtgccc
    13321 tcttatctgg agatcagaaa agatctgcca cgtaattgct cggggaaaat aattagaaag
    13381 aatctgaaat aaatgaaacg gcagggaact accctgccag ctaataatca cgctctaaaa
    13441 caatatgcat tttttgcagg gatgttgtcg tccctgaaaa agcaaaaatg gagaaaagga
    13501 atgagtgaat cattacatct gacccgcaat ggatcaattc tggaaattac ccttgatcgc
    13561 ccaaaagcga atgctattga tgcaaaaacc agctttgaaa tgggcgaagt atttctaaat
    13621 ttccgtgacg atccgcaatt acgtgtcgcc attattaccg gtgccggaga gaaattcttt
    13681 tccgcgggct gggatttaaa agcggcagca gaaggcgaag caccggatgc tgactttggc
    13741 ccgggtggtt ttgcaggatt aaccgaaatt ttcaatctcg acaaaccggt tatcgccgct
    13801 gtgaacggct atgcctttgg cggcggcttt gaactggcgc tggcggcaga ttttattgtc
    13861 tgtgccgata atgccagctt cgccctgccg gaagccaaac tgggcatcgt tcctgacagc
    13921 ggcggcgtgc tgcgtctgcc gaagatcctg ccgcctgcca tcgtcaatga aatggtgatg
    13981 accggcagac gaatgggcgc agaagaggcg ctgcgttggg ggatagtcaa ccgcgtggtt
    14041 agccaggcgg aactgatgga taacgcccgc gaactggcgc agcagctggt taacagcgct
    14101 ccgctggcga ttgcggcgct gaaagagatc taccgcacca ccagcgaaat gccgatagaa
    14161 gaagcgtatc gctatattcg cagcggcgtg ttgaaacact atccgtcggt tctgcattcg
    14221 gaagatgcca ttgaagggcc gctggcgttt gccgagaagc gcgatccagt gtggaaagga
    14281 cgttaacaac cgtgagctat tacgcctttg aggggttaat tccggtggtt cacccgacgg
    14341 cgtttgtcca tcccagtgca gtattgattg gcgatgtgat tgtggaagcc ggtgtctaca
    14401 tcggcccact cgcctcactg cgtggtgact acgggcggtt gatcgtgcaa gcgggagcca
    14461 atattcagga tggctgcatt atgcatggct actgcgacac tgacaccatc gttggggaaa
    14521 acggccatat cgggcacgga gcgatcctgc atggttgtgt gattggtcgc gatgcattgg
    14581 tcgggatgaa cagcgtgatt atggatggcg cggtcattgg cgaagagagc attgttgccg
    14641 ccatgagctt tgtcaaagca ggctttagcg gcgagaaacg ccagttgttg ataggtacgc
    14701 cagcccgcgc tgtacgcagt gttagtgacg acgagttaca ctggaaacgg ttgaatacca
    14761 aagagtatca ggatcttgtc gggcgctgtc atgcatcgtt gcatgaaacg cagccactga
    14821 ggcaaatgga ggaaaatcgc ccccgtttgc aggggacgac ggatgtgacg ccgaaacggt
    14881 aatcttgccg gatgcggcgt aaacgcctta tccgacctgg ctggaaagga accgtaggcc
    14941 gtataagatg caccagcatc gcatccggcg accaggtcgc acggtatcaa cgaaacgaca
    15001 ttaacgacgc atactctttg acaacatcat ctgccacttc tgctcgcggt taagttccgt
    15061 gagcggaaca gcaggaatac gcggtgtctt tcttacgccg actaattttt cccgactgtt
    15121 attgcgcagg cgcgcgtaaa tttgctcatc gatatcgacc actttaacca gtcgctgaca
    15181 ctggcatccc cgcccttcca gcttattagg gatcatctta acttcgcagc ttatttctgt
    15241 gacttccgac agaatataag tcagggtatt aaccgccttg ctgtgttcaa tatcgaaatg
    15301 aatagagatc tctcttgcta tcacccaccg attttcagcc atcatccatt cggcgattaa
    15361 caagtagagt ggtttttcaa catatccttc acacatacga actccagttt cacaaattaa
    15421 aagtctggct atgactcgtt ttgaagcaat taaacaaggc catattaaaa ttgtggatat
    15481 atcgatagta tgcaacttca ccgtcgataa gtgtgagcta aatcctgctt atgtcatcaa
    15541 aaatatcgat tccccaaaag atttacttaa tgggcaaaaa aaacggtcct catcagagaa
    15601 ccgtattagt tattcgatca aattagcgga tgaaaaatat ccgccatgac acgctattat
    15661 ttgatctgcg cgtgcatttc ctgcaccgaa attacttttt cagtcgcatc ggcattcagc
    15721 gccatcgcgg tagcgaaacc accgttcagg gtggtgtcgt aatgcacttt atattgcagc
    15781 gcactgcgac gaatcacgcg ggagtcttca atcgcgcgac gacctgaagt ggtgttgatg
    15841 atgtaggtat attcgccatt cttgatgcgg tcctgaatgt gcggacgacc ttcatgcacc
    15901 ttgttcacca gacgcggatt gatacccgct tcgcccagca caatcgccgt gccgtgggtc
    15961 gcatccagct cgaaaccctg tttcagcagt tttgccgcca ggtccaccac gcgttctttg
    16021 tcgccttcgc gcacggaaag cagcgcacga ccgtgtttct tcatggtgga gttgctgccc
    16081 agctgcgctt tggcgaacgc ttcagcgaag gtgcggccca cgcccatgac ttccccggta
    16141 gagcgcattt ctggccctaa cagcgggtca acgcccggga atttgttgaa cggcagcacc
    16201 acttctttca ccgagtagta cggcgggata acttctttgg ttacgccctg ctcagccagc
    16261 gatttgccag ccatcacacg cgccgccact tttgccagcg gtacgccggt ggctttggag
    16321 acgaacggaa cggtacgcgc cgcacgcggg ttaacttcaa tcaggtagac ttcgttgttt
    16381 ttaaccgcaa actgtacgtt catcaggccg cgcacctgca attcgaaggc cagtttctgc
    16441 acctgctggc gcatcacatc ctgaatttcc tgacttaaag tgtaggctgg tagcgaacat
    16501 gcagagtcac cggagtgcac gcccgcctgc tcgatgtgct ccatgatgcc gccaatcagc
    16561 accatttcac cgtcgcagat ggcatccacg tcaacttcta ctgcgtcatc aaggaaatga
    16621 tccagcaaca ctggcgcatc gttagacacg ctgaccgccg tctggaagta gcgacgcagg
    16681 tcagcttcgt catagacgat ttccatcgcc cgaccgccga gaacgtaaga cggacgtacc
    16741 accagcgggt agccaatctc tttcgccttc tctaccgcca tttcaatagc ggtaacggtg
    16801 gcgttcgccg gttgtttcag tttcagacgg tcaaccgcat gctggaagcg ttcacggtct
    16861 tctgcacgat cgatagcatc cgggctggta ccgataaccg gtacgccagc cgcttccagc
    16921 gcgcgcgcca gtttcagcgg ggtctgaccg ccgtattgga cgataacgcc tttcggcttc
    16981 tcaatacgca cgatttccag cacatcttcc agagttaccg gctcgaagta gaggcgatcg
    17041 gaagtgtcgt aatcagtgga gacggtttcc gggttacagt taaccataat ggtttcgtaa
    17101 ccgtcttcgc gcagcgccag cgaggcgtgt acgcagcagt agtcgaattc gataccctga
    17161 ccgatacggt tcgggccgcc gccaagcacc atgatttttt cacggtcggt agacggattc
    17221 gcttcgcact cttcttcata agtggagtac atgtaagcgg tgtcggtggc gaactctgcc
    17281 gcacaggtat ccacgcgctt ataaaccggg tgcaggtcat actggtcgcg cagcttgcgg
    17341 atttctgctt cacgcacgcc cgcgagtttt gccagacgcg catcggcaaa gcctttgcgt
    17401 ttcagctggc gcaggaattc agcgttcagg ccagtgatgc ccacttctgc gactttctct
    17461 tccagacgca ccagctcttc aatttgtacc aggaaccagc ggtcaatgtt ggtcaggttg
    17521 aacacgccgt ctacggacag gcccgcgcgg aaagcatcgg cgatatacca gatacgctct
    17581 gcgcctgcat ctttcagttc gcggcggatt ttggtcaacg cttccgggtc gtccaggctc
    17641 actttcgggt cgaagccagt cgcgcccact tccaggccgc gcaatgcttt ttgcagggat
    17701 tcctgttgcg tgcgaccaat cgccatcact tcgccaacag atttcatctg agtggtcaga
    17761 cggtcgttag caccggcgaa tttctcgaag ttgaagcgag gaattttggt aaccacgtaa
    17821 tcgatagacg gctcgaagga ggccggagta cgcccgccgg tgatgtcgtt catcagctcg
    17881 tcgagggtgt aacctaccgc cagtttcgcc gctactttgg caatcgggaa gccggtggct
    17941 ttcgacgcca gcgcggaaga acgcgacacg cgcgggttca tttcgataac aatcaggcga
    18001 ccgtttttcg ggttaaccgc aaactggacg ttggaaccgc cggtttcaac gccgatttca
    18061 cgcagcaccg ccatcgaggc attacgcatg atttgatatt ctttatcggt cagcgtttgg
    18121 gctggcgcga cagtgatgga gtcaccggtg tggatgccca tcgcatcgaa gttttcgata
    18181 gagcagacga tgatgcagtt gtcgttttta tcacgcacca cttccatctc gtactctttc
    18241 cagccgatca gcgactcatc aatcagcaac tctttggttg gggagagatc cagaccgcgg
    18301 gcgcaaattt cttcaaactc ttcgcggtta taagcgatac cgcctccgct accgcccatg
    18361 gtaaaggatg gacgaataat gcatgggaag cccacgtcag cggcaaccgc cagcgcttct
    18421 tccatggtgt gcgcgatacc ggaacgcgcg gtttcgagac caattttctt catcgctacg
    18481 tcgaaacgac ggcggtcttc tgctttatca atcgcgtcgg cggtggcacc aatcatggtg
    18541 acgccgaact cttccaacac gccctgacgt tccagctcca gagcacagtt cagcgccgtc
    18601 tgaccgccca tcgttggcag caccgcgtcc gggcgctctt tttcaataat cttgcgtacc
    18661 acttcccagt gaatcggctc gatgtaggtc gcatcagcca tttccgggtc ggtcatgatg
    18721 gtcgccgggt tagagttcac cagaatgacg cggtaacctt cttcacgcag ggctttacat
    18781 gcttgcgcgc cagagtagtc aaactcacac gcctgaccga taacaatcgg gcccgcaccc
    18841 agaatcagga tactttttat atctgtacgt tttggcatgg ctcttttact cctgattact
    18901 tagcggtttt acggtactgc tcaattaact cgataaagtg gtcgaacaac ggcgcggcgt
    18961 cgtgtggacc agggctggct tccgggtgcc cctggaagct gaacgccggt ttatcggtgc
    19021 gatgaatgcc ctgtaacgta ccgtcgaaca gggatttatg cgtgacacgc aggtttgcag
    19081 gtaatgttgc ttcatccacc gcaaaaccgt ggttctgggc ggtgatcatc accacgtttt
    19141 tctccacatc tttaaccgga tggttgccgc cgtggtgacc aaatttcatt ttgacagtct
    19201 tcgcaccgct cgccagcgcc agcagctgat gaccgaggca gatgccgaag accggaatat
    19261 cggtttcgag gaatttctgg atggcggtaa tggcgtaatc gcacggggcc gggtcgccag
    19321 gaccgttgga gaggaagatg ccgtctggat tcattttcag cacatcttcc gcagaagttt
    19381 gcgccggaac gatggtcagg cgacagcctc tatccaccag catccgcagg atgttgcgct
    19441 tggcaccaaa atcataagcc acgacgtgga acggcagctc gtcttctttt ttcgcttctg
    19501 gcaggccgcc ggtcaacgtc cagctccctt gtgtccagct ataggcttct gcggtggtca
    19561 cttcttttgc cagatccatg ccgttcagac ctgggaacgc gcgggctttt tctaacgcca
    19621 gcgccgcatc cgggttatcg cctgcgataa tgcagccatt ctgtgcgcct ttctcgcgca
    19681 gtaaacgcgt cagcttacgg gtatcgatat cggcaatcgc cacgatgtta tggcgtttca
    19741 ggtaagaaga gaggtcttcg gtattacgga agttgctggc aatcagcggc aggtcgcgaa
    19801 tcaccagacc ttgtgcatgt acctgagaag attcttcatc ggtgtcatta gtgccgacat
    19861 tgccaatatg gggataagta agagtaacga tttggcgaga ataggaagga tcagtgagga
    19921 tttcttgata accggtcatt gaagtattga aaacgacttc cccaaccgcc gaacctgttg
    19981 cccctatggc ccgaccgtga aactgggttc cgtcttccag aaccaatagc gctgacttaa
    20041 tcaaaacacc ctccagagaa tattcactca ctttatttgc atattaattc acaatgatga
    20101 cgtaaatcaa tgcaaatctg cttaccgacg aatttctggc aaacggcggc attctggaga
    20161 tatgggcgct aaaagtcaac ttaatggcgg gattttttat attattttgt ggctcatgca
    20221 tgttttacag acattaagcg acaaaaagat catataaccc gttctggaaa acgcttgcgc
    20281 agcaagaaga ttgcatgaaa taaaaatgag cagaaaaaag tggaccagat ggtcaaaatt
    20341 tacattaaaa caacaaaaac agaagacaaa acgtgtaatt tttagcactt acatgaaatt
    20401 taaaaacata ataccaaaaa taaaagggct aaaaattaaa gccctttatg atcaataaat
    20461 taaatgtgtt atttttgcat catcataaac attttgtggt tacaaattat tgagatcaag
    20521 cacatctcgc atatcaaaaa gaccactttc cttaccactc aaccacaaag ccgatcttac
    20581 cgcaccgtta gcaaatgtca tgcggctgga cgccttatgg gtgatctcca gacgctcgcc
    20641 aatatcggca aacatcgcgg tatgttcgcc aacgatgtca cctccacgca cggtggcaaa
    20701 accaatggtg ccaggcacac gttcaccggt atggccttca cgactgtaga ccgcgcaatc
    20761 tttcagatct ttatcaaggg cgtgggcgat cgcctctccc attgccagtg cggtgcctga
    20821 cggcgcatca actttatgtc tatgatgtgc ttcaataatt tcgatatcgg tgtagtcacc
    20881 catcactttg gctgctttct ccagcagctt aagcatgacg ttaacgccaa cgctaaaatt
    20941 ggcggcaaag acaatcgcaa tatcggcggc ggcgtcacga atcgcctgtt taccggcttc
    21001 gtcaaacccc gtagtgccga tcaccatccc tttgccatgc tggcgacaaa aagcgagatg
    21061 gttcagcgta ccttccggac gggtaaaatc gataaacaca tcaaaatcat cttttatcgc
    21121 atcaaggctg ctttgcacgg taacgcctgt tttcccggct ccggccagct caccggcgtc
    21181 gctgcccagt aaagaagatc cttcacgctc cagcgcagcg cccagttgca cgccctctaa
    21241 tgccagcgcc gcctgaatca actggcggcc catacgcccc ccggctcccg cgatggcaac
    21301 gcggatgttt gcatcatgca tagctattct cttttgttaa tttgcataga ccgttttcag
    21361 agtaaccagc gcatcgctac gccgccagcc aaaaacgctg ataattagaa attaatgata
    21421 aacagggagg aatatcagtg aaaggcatga cgcgccaacc actgcgtcac gccgtctgtc
    21481 attatgcctg ctcttggggt tttgacggca ggcagcacag tataaacaga attacgcctg
    21541 cataagggat caacacgccg atggaaaacc accatcctga acgtccgata tcgtgcatcc
    21601 ttctgaagcc taacgccatt cccggcacgg caaaaatcaa cgttaaaata aatgatgtgg
    21661 gcagcgcaaa aaaaatgaaa gcgccataat catcgtcaat ggttattgca aacaaccaac
    21721 ttaaaagcgg tagcaggcaa attattgcgc taccgatgat aaacgaccag aactcaaatc
    21781 gggtagcaac gccattataa ttaaagatat ttttccaccc tgccagatat gcatcgccat
    21841 acgtcattaa aacctccttg ttttattatt agagcgccag agccagcacc tcagctaccc
    21901 actgctggaa gcctcttaca tccagatcca gcgccacctg cacgtttgaa ggtttgccca
    21961 ggcaaccgtc gatatcaaca accgtcgtgc ctgaggtaaa ttcgccctga gtttccactg
    22021 ccacaaaaca cggtttgaga gtgaacaggt ccgggcgcac cagccaggca atagcgcaaa
    22081 gatcgtgcat acgtagtcca ctttgcatac tgccgctacg gtagtggcta aacagggcgt
    22141 gtagcatttt tccggtgcga ttcaactccg gcaaagtagc gagatagtca ggggttaata
    22201 ttgcctgatt ggtgacatcc agaccgcaca tgacgatttc gatgccgcta cggaagacac
    22261 aggctgccgc ttctggatcg gcagcgatat taaactcggc gtttggtgtg cagttgccgc
    22321 gaccggcgga accgcccatg atcaccagac ggcgaatatg cggtttgcat tccgggcatt
    22381 gtgaaagtaa cagcgcaata ttggttaacg ggccgatggc caccagcgta acaggctctg
    22441 gtgcacgcat cagggcatca cgaatcgcca gaaacgctgg tatctcgagt ggctttcggt
    22501 tgtgctcaac aaagtcgtag ccagccattc ccgattcgcc gtgcacagat gctgcattac
    22561 gcggtgcgcg taccagcggc acagcggctc cctgggcgag cggaatctcc gcattccaga
    22621 aatgcagcag ttgcagggcg ttgcgggtcg ttttctcaac cgaaacatta cccgcgacgg
    22681 tggtcatcag ttgcaggtcg agttcggggg caaaaatcgc ggcggcaatg gcgaccgcat
    22741 cgtcaatgcc ggggtctgta tcgaggaaga taggtaaacg catgttttct ccataaaaaa
    22801 tgccggtaac aagaccggca ttttcgcata acttaggctg ctaatgactt aatcgacttc
    22861 acgaatatcg acacgcagct ctttcggcac ttcgaaaaca atattttctt cacggccttc
    22921 cagcggaatg gcttcaccac cgcccagctg ttgcaaacgt gccaccacat tctgcaccag
    22981 aatatccgga gccgatgcgc ccgcagtcac gccgacgcat ttaacctctt tcacccactc
    23041 ttcctggata tctttcgcat cgtcaatcaa aaacgcgcgt ttgcccatcc gctgggccag
    23101 ctccgccaga cggttggagt tagaggagtt tttcgaaccg accaccagca ccacttccgc
    23161 ctgctctgcc agggcgcgta ccgcttcctg acggttagtc gtggcgtagc agatgtcatc
    23221 tttgcgcgga ccgacaattt tcgggaagcg tttacgcagc gcgtcgatca catcagacgt
    23281 gtcatccacc gacagcgtgg tttgggtcat aaaggagagc ttctcttcgt ttttgaccgt
    23341 cagcttccat acatcgtctg gcgattcaac cagatacatt cccccttccg ggttgctgta
    23401 ctgccccatc gtcccttcca cttccgggtg cccggcgtgg ccgatgagaa tagattcttc
    23461 gccacggcga ctggcgcggg cgacttccat atgcactttg gtcactagcg gacaggtggc
    23521 gtcgaatacc gtcaaatcac ggcttttcgc ttcgttacgt accgcctgag aaacaccgtg
    23581 tgcagagaaa atcaggattg cgccgtccgg cacttcgctg atctgctcaa taaagatagc
    23641 cccacgctcg cgcaggctat cgaccacgta gcggttatgc accacttcgt gacggacata
    23701 aatcggtgcg ccgtaaatgg ccagcgcgtt ttcaacaatg ctgatagcgc ggtctacccc
    23761 ggcacaaaaa ccacgtgggt tggccaacag gatctgcatg ttacgcctcc agtgccggat
    23821 caatttccag cacttcaata tcaaaatgaa tggtctgccc ggccagcgga tgattgaaat
    23881 caacggtaat ggagtcaccg ttaatttcgc ggatcacgcc aggcatctca ctgccatcca
    23941 ttgcggtaaa aagcatgatt gcgccaattt ctggctcgcc tgcatccata aattcacggc
    24001 gggagaagta ctgaatcagg tccggtgacg gcacgccaaa cgccgcatcg ggctccagcg
    24061 agaaggtggt tttatcgccc actttcagcc ccaacaggtg ttgctccagc ccttcagaaa
    24121 gagaagcatc gcccaggcgg aacagcgccg gtttaccgtt gttgcgggta gactcggcgg
    24181 tggtgccatc gtcgagtttt agcgtgaaat gcaccaggac ggcgctattg ctctgtacag
    24241 attcagacat gcaggtttgc tcttatattt tgaggatttt tgccggatgc ggtctacgat
    24301 ttcgcagcaa tctgtaggcc gcatccggca gggtttatta ttgttttttc gctttagaag
    24361 gtaaaaaccc ttccagcacg atcagtgccg caccgacaca aatggcagta tcggcaaggt
    24421 tgaaggtggc gaagtgccag tcgccgacgt agaagtcgat catatcgaca acgaagccgt
    24481 gccacaggcg gtcgaacagg ttgcccagcg cgccgccaat aatcagcgcg taagcgatat
    24541 tgtttagctt ctgcgtggcc ttcgagcgat acatcatcac tgccaggatc acgctaatac
    24601 caatcgcaat accggcaaag aaccaacgct gccagccgcc gctatcggca aggaaactaa
    24661 acgccgcgcc atagttacgc gcataatgca gattaagcga cgggaacagc gggaccgtat
    24721 cccccagagc aaagttctgg aggatcaggt acttgctgcc cagatcgata atcagcacga
    24781 ctaccaccag ccacagccag cgtagccctg ttgaacagat cgattgactc atcaggcaaa
    24841 cttacgtttt tcaccgtcac cggcgacgtt gctgacacag cggccgcaga tttctgcgtg
    24901 ttccgctacc ttgccgacat cctgggtgta gtgccagcag cgtgggcact tctcaccttc
    24961 ggctttactc aacgcgactt tcagcccttt gagtacttcg ctctgctggg catcagcagg
    25021 tgcgtcgtta tagtctgcaa cggtagcgcc ggaggtcaac aggacaaatc gtaattcatc
    25081 gcccagcgcg gtcagtttcg ccgccagttc cggttctgcg aacaaggtca ctgccgcttc
    25141 cagcgagcca cccactttct tgtcggcacg cgcttgctca atgactttgt tcacttcgcc
    25201 acgcactttc aacagctcgt cccagaacgc atcgttcatt gcttcactgt ctgccagacc
    25261 aaacaggcct tcgtaccact cgccggtgaa gacgtatttt tcacgttcac ccggcaggta
    25321 gccccacact tcatcagcgg tgaaggagag gattggtgcc atccagcgca ccagcgcttc
    25381 cgcgatgtga tacagcgcag tctggcagct acgacgcgcc acactgtccg ctttggcggt
    25441 gtactgacgg tctttgatga tgtcgaggta gaaggaaccc atctcaacgg agcagaagcg
    25501 catcagacgc tgtaccactt cgtggaaatc gtatgcttcg tacgccttga ggatgtcttc
    25561 ctgtgccgct ttcgcacaac ctacggccca gcgatccagt actaccatct cttccggttt
    25621 caccatatct tttgccggat caaaaccgtt caggtttgcc agcaggaagc gcgcggtgtt
    25681 acggatacga cgatagctgt cggcagcgcg tttcaggatc tcgtcagaaa cggccatttc
    25741 accggtgtag tcggttgatg ccacccacag acgcagaata tccgcgccca gtttgttcat
    25801 cacatcctgc ggcgaaacgg tgttgccgat ggatttagac atcttgcggc cctgaccatc
    25861 cacggtaaag ccgtgggtca gtacctgacg atacggtgct ttgcccttca tcgcggtaga
    25921 gatcatcaga gaggacatga accagccacg gtgctggtca gaaccttcca gatacatgtc
    25981 cgctgcgtga ccggcaaatt ccggacgcac gtcaacaacg gaagagtggg tagatccgga
    26041 gtcaaaccat acgtcgagag tatccggcac tttcacgtac tgatcagctt catcgccgag
    26101 gatctctttc gcatcgagat cccaccacgc ctggatgccg tcaacttcaa cgcgtttagc
    26161 cacttcttcc atcagttcga gggtacgcgg atgcagctct tccgtgtctt tgtgcacgaa
    26221 cagtgacatc ggtacgcccc aggtgcgctg acgggagata caccagtcag gacggttagc
    26281 aaccatcgac tcgatacgcg cctggcccca gtccgggatc cactgcacgc ctttgatctc
    26341 tttcagtgac tgcgcacgca gacctttctg atccatgctg acgaaccact gcggcgtcgc
    26401 gcggaagatg atcggcgttt tgtgacgcca gcagcacgga tagctgtgct gcattttctc
    26461 aacgtgcaac agcgcgcctt tttcctgcag cagcgcaaca acgatgtcgt tcgctttgaa
    26521 gacgttcacg ccatccagtg tcgggtaagt gcccgccaga taagtgccgt ccgggccaac
    26581 cgggttagcg gtttccaggc cgtatttctg accgatcaca tagtcgtccg ggccgtggcc
    26641 aggcgcggtg tgaacggcac cggtaccggc atccagggta acgtgatcgc cgaggattgc
    26701 aggaacgtcg aagcccataa acggatgggt aaagcgcagc agctcaagct ccgcaccttt
    26761 taccgtgccg agaatggtgt aatcggtcac gccgatacgc tgcattacgc tttcaaccag
    26821 atctttcgcc agaatcacgg cctgaccgtc gatctgcacc agcgcatagt cgaaatcagg
    26881 tgcaatagag attgcgcggt tcgcaggcaa ggtccacggc gtagtggtcc agatcaccag
    26941 cgagattggg ccgttaacgt tgcttacggc aaattttgct atcagtgcat cctgatcgac
    27001 cgcctggaaa gcaacgtcga tggacggaga agttttgtcg taatactcaa cttccgcttc
    27061 cgccagcgca gaacggcagt caacgcacca gtgtaccggc ttcgcgcctt tgtgcaggtg
    27121 accgttgccg atgattttgc ccagcgcgcg gatgatatta gcttcagttt tgaaatccat
    27181 ggtcaggtac gggtgggacc agtcgcccag cacgcccaga cggataaagt ctttgcgctg
    27241 accgtcaacc tgggtcgccg cgtattcgcg gcacttggcg cggaactcgg cggcggtaaa
    27301 tttctcaccc ggcttaccgt attcttgctc tactttcagc tcgatcggca gaccgtggca
    27361 gtcccagcca ggcacatacg gcgagtcata accggaaagc cctttggact tcacgataat
    27421 gtctttcaga atcttgttaa ccgagtgacc aatatgaatg ctgccattcg cataaggagg
    27481 gccatcatgc agaatgaagg tttttttgcc ttttttagcc gcacgaatga tgccgtacag
    27541 atcatcatca gtccaacgcg ccagcattcc cggttcgcgc ttggcgagat cgccacgcat
    27601 cgggaaccct gtttccggca aattcagggt tgatttatag tcactcatca gattctcggt
    27661 tccgtgtttc ggtttgatta cataacaggc ttaagccggt tttgttagcc caaaaaattc
    27721 gcgggcggtt aattcatcac gcgcaatctg cgctttcagt tcgtccagcg acgcaaatcg
    27781 ctgctcattg cgtatttttt tacgcagcac tacttgtata tggcgaccgt aaaggtccat
    27841 tgcaacatct aacaaatgca cttccagctg ctggcgaata ccggcaaccg ttgggcgtgt
    27901 tccgatgttt gccacgccgg gtaacggctt ttcaccgagg cccagcactt ctaccgcata
    27961 aacccctttc accggggaaa cctgacggcg tagcggtaca ttcgccgtcg ggaaacctat
    28021 agtgcgccct aattcatcac cgtggaccac acgcccggag atggcaaacg ggtgccccag
    28081 taaactttct gccagagcca gattgtcatc cgcaagagcc tgacgcacgg cggtgctgct
    28141 gatacgcacg ccaccttcgc aaaaagtttg cgtactggtg atatcgaagc cgtattccat
    28201 gccagctttc tgtaataaca agaaatcgcc ttcacgacca gcgccaaagc ggaaatcatc
    28261 ccctacagca agaaatttta cgcgcaaatg cttcaccaga agatcactga tgaaattttg
    28321 cgcggttaac gccgcgaaac gcctgtcgaa acgcacgcac agcacgtaat caacgccaca
    28381 ctctgcaagg taacgcagtt tttcccgcag tcgggtcagc cgtgccgggg ctttatcggt
    28441 agcaaacagt tccagtggtt gaggttcaaa aagcatcacc atcaccggta agttgcgctt
    28501 gcgcccttct tcctgcaagc cctgtaacag cgcgcgatga ccgcgatgca cgccgtcgaa
    28561 attaccaata gtcagcacac acccttcttg cggggcctgg ctgagattat gtatgccgcg
    28621 tatcagcttc atgtctggct caaaacagtg aaaatcgtcc gagtatacct tgtacagcgg
    28681 tcaaggttaa ccggcgattg agtaccgaaa tcagaaagaa gtggcttttc attgccatgg
    28741 cgcaaatcac gggaagaaac tgaccacctg ctgcaatttt tatcgcggaa aagctgtatt
    28801 cacaccccgc aagctggtag aatcctgcgc catcactacg taacgagtgc cggcacatta
    28861 acggcgctta tttgcacaaa tccattgaca aaagaaggct aaaagggcat attcctcggc
    28921 ctttgaattg tccatataga acacatttgg gagttggacc ttggctaata tcaaatcagc
    28981 taagaagcgc gccattcagt ctgaaaaggc tcgtaagcac aacgcaagcc gtcgctctat
    29041 gatgcgtact ttcatcaaga aagtatacgc agctatcgaa gctggcgaca aagctgctgc
    29101 acagaaagca tttaacgaaa tgcaaccgat cgtggaccgt caggctgcta aaggtctgat
    29161 ccacaaaaac aaagctgcac gtcataaggc taacctgact gcacagatca acaagctggc
    29221 ttaatcgcca atttgctgaa gctttgtgaa aaagcccgcg caagcgggtt tttttatacc
    29281 tgctgctttg tgatgtgtga gaaaacacac tgccggttgc cagatagtca aaagatgctg
    29341 gttcttgcag acatgcagtt acactctctg aactcttcgt gacttaaaaa tcgtagaatc
    29401 atcggttgat acattctgaa taagaatatc tactcaatct tcagactccc cctacacaga
    29461 atgtggagat tgtgatacac attgaggtat taaacttaag accactctat ttttgaatta
    29521 aattcctata cactttaacc attcataaat atctccttat tagaccttgt aaattattga
    29581 cagcaatcaa cacaggttaa ctacctattt atattgtact tcttaaaatt aatgagcgat
    29641 ttttgatagt agtcacattc actcataaat taaattaata aaaattcatc aacttcaagg
    29701 actgataatg acagatggta tctcaacttc gccacattgt ctttataaat caaatatagc
    29761 agacgatgta ataataaata aaacaagaca aaatgagtta gtaaaagtgt tttgcgagta
    29821 taaaacagag ttcttaattt tgtttgatga cttttttaga agccaggatt tgcctaaacc
    29881 atctccagtc cttcatcatt tttttcaata cactcatcta agagatgcgc atttttatag
    29941 atgtaaactc atagaacata cagttcaatt ttcttttttt aaacacaaag aaatcacatt
    30001 acttagattg gatgtttttg atgacagaac aagcgaatgt ctttctgaag aaattaaaac
    30061 atatcaagaa tgctatgaaa aattcattaa gtttttaaaa gcaaatttca accaggaaat
    30121 atacccgcag ctatatattc ctgagatatt ttatgacgcc tgcaaacatc tccagtcatt
    30181 tttttatcat caggagaagt cacaaaacgc t
//
LOCUS       DAPOZO010000060        30111 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPOZO010000060 DAPOZO010000000
VERSION     DAPOZO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.060"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(118..1932)
                     /locus_tag="ROI21_002886"
     CDS             complement(118..1932)
                     /locus_tag="ROI21_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111787.1"
                     /translation="MVANINLIKQESYSIVNLEKQLMSNESPKQTLLNDFSHRLKMEG
                     EEQLCDCVNELKLGLSSNNKYDLFKNKETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTFDVGEVINSYN
                     IDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAKLPQERS
                     EKVEDMCERMRGGISMFNHTTKTAGNIEHNLHLSFLVDSRPSITNKYSAKDVGEDILA
                     LPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIL
                     DLRFLSAIKQKVFLGSEKPLFVKHTAMMREACEELPQSVEYVPLACEAHSHRSVALAK
                     TVAAPIMATGVGSALMYATSSGPYYAQSLSGSPDIEMIKNRMAQLFTQLDASVRNKYR
                     PAFDKWNELVDKLNHERNTVPFACLTTILATAINETPEGDTNVAVVMGCKSAKDRTIS
                     IVLGNSMLQTLLEKRLADGREIEKLFDQQGYFSCDSLTAKELMMLKDLFDIRVLHLSN
                     KFNVGLQGNINTDVLQDSFFKNVDFIHESSSYTVGMGV"
     gene            complement(2348..3289)
                     /gene="fabY"
                     /locus_tag="ROI21_002887"
                     /gene_synonym="yiiD"
     CDS             complement(2348..3289)
                     /gene="fabY"
                     /locus_tag="ROI21_002887"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDX5111788.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTTPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3334..3771)
                     /gene="dtd"
                     /locus_tag="ROI21_002888"
     CDS             complement(3334..3771)
                     /gene="dtd"
                     /locus_tag="ROI21_002888"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDX5111789.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3768..4640)
                     /locus_tag="ROI21_002889"
     CDS             complement(3768..4640)
                     /locus_tag="ROI21_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDX5111790.1"
                     /translation="MLKTIHEKARYRTRPLWAWLKLLWQRINEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQQYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4634..5233)
                     /gene="yihX"
                     /locus_tag="ROI21_002890"
     CDS             complement(4634..5233)
                     /gene="yihX"
                     /locus_tag="ROI21_002890"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDX5111791.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEAMCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5332..6117)
                     /locus_tag="ROI21_002891"
     CDS             complement(5332..6117)
                     /locus_tag="ROI21_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDX5111792.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNMAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(6151..7047)
                     /gene="yihV"
                     /locus_tag="ROI21_002892"
     CDS             complement(6151..7047)
                     /gene="yihV"
                     /locus_tag="ROI21_002892"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HDX5111793.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGNLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7215..8111
                     /gene="yihU"
                     /locus_tag="ROI21_002893"
     CDS             7215..8111
                     /gene="yihU"
                     /locus_tag="ROI21_002893"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDX5111794.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWSNKVLSGDLSPAFMIDLAHKDLSIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8135..9013
                     /gene="yihT"
                     /locus_tag="ROI21_002894"
     CDS             8135..9013
                     /gene="yihT"
                     /locus_tag="ROI21_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HDX5111795.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            9029..10270
                     /gene="yihS"
                     /locus_tag="ROI21_002895"
     CDS             9029..10270
                     /gene="yihS"
                     /locus_tag="ROI21_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HDX5111796.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYAIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTATGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDVNAK"
     gene            10338..11240
                     /locus_tag="ROI21_002896"
     CDS             10338..11240
                     /locus_tag="ROI21_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HDX5111797.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQDYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDDYLFQLPAEQVYAVDEHANPTTLHHVDELNLNFTRATKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            11301..12176
                     /locus_tag="ROI21_002897"
     CDS             11301..12176
                     /locus_tag="ROI21_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDX5111798.1"
                     /translation="MDFFTLFSPDGAMVETSVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRNWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRAEESK"
     gene            12284..14320
                     /gene="yihQ"
                     /locus_tag="ROI21_002898"
     CDS             12284..14320
                     /gene="yihQ"
                     /locus_tag="ROI21_002898"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HDX5111799.1"
                     /translation="MDTPRPQLPDFQFHQNNDCFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            14366..15751
                     /locus_tag="ROI21_002899"
     CDS             14366..15751
                     /locus_tag="ROI21_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5111800.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQIGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15794..17197
                     /locus_tag="ROI21_002900"
     CDS             15794..17197
                     /locus_tag="ROI21_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5111801.1"
                     /translation="MSDHNPLTLRLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            17264..17956
                     /gene="ompL"
                     /locus_tag="ROI21_002901"
     CDS             17264..17956
                     /gene="ompL"
                     /locus_tag="ROI21_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HDX5111802.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(18048..19313)
                     /locus_tag="ROI21_002902"
     CDS             complement(18048..19313)
                     /locus_tag="ROI21_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5111803.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(19415..20395)
                     /locus_tag="ROI21_002903"
     CDS             complement(19415..20395)
                     /locus_tag="ROI21_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDX5111804.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIS
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(20403..21113)
                     /locus_tag="ROI21_002904"
     CDS             complement(20403..21113)
                     /locus_tag="ROI21_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDX5111805.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(21330..23153)
                     /gene="typA"
                     /locus_tag="ROI21_002905"
     CDS             complement(21330..23153)
                     /gene="typA"
                     /locus_tag="ROI21_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDX5111806.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            23526..24935
                     /gene="glnA"
                     /locus_tag="ROI21_002906"
     CDS             23526..24935
                     /gene="glnA"
                     /locus_tag="ROI21_002906"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDX5111807.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            25109..26158
                     /gene="glnL"
                     /locus_tag="ROI21_002907"
     CDS             25109..26158
                     /gene="glnL"
                     /locus_tag="ROI21_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDX5111808.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            26170..27579
                     /gene="glnG"
                     /locus_tag="ROI21_002908"
     CDS             26170..27579
                     /gene="glnG"
                     /locus_tag="ROI21_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDX5111809.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            27691..27801
                     /locus_tag="ROI21_002909"
     CDS             27691..27801
                     /locus_tag="ROI21_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDX5111810.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(27991..29364)
                     /gene="hemN"
                     /locus_tag="ROI21_002910"
     CDS             complement(27991..29364)
                     /gene="hemN"
                     /locus_tag="ROI21_002910"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDX5111811.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RKVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(29553..30062)
                     /gene="yihI"
                     /locus_tag="ROI21_002911"
     CDS             complement(29553..30062)
                     /gene="yihI"
                     /locus_tag="ROI21_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDX5111812.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
BASE COUNT         7390 a         7699 c         7361 g         7661 t
ORIGIN      
        1 gctttctgta tggcttcgcc atgcccctcg cagctaagtg cctgcgcaca tctctctttg
       61 tatgtcattt ttgcaataca ttgtccgaat tctgataatc ctaatgagtt ttacccgtca
      121 gactcccatc cctacggtat acgagctaga ttcatgaatg aaatcgacat ttttaaaaaa
      181 cgaatcttgt aaaacatccg tgttgatatt accttgcaat ccaacattaa atttgttaga
      241 gagatgaaga actcgaatat caaaaaggtc ttttaacatc attaattctt tggctgttaa
      301 tgaatcgcaa ctaaaatatc cctgttgatc aaaaagcttt tctatttctc taccatcggc
      361 gaggcgtttt tcaaggagtg tttgcaacat cgagttacca aggacaatgg atatcgtgcg
      421 atctttcgca gatttacacc ccatcacaac ggcaacattc gtatccccct caggagtttc
      481 atttatcgcc gtcgctaaaa tggtggtcaa acacgcaaac ggaactgtat ttctttcgtg
      541 atttaattta tctaccagct cattccattt atcaaaagcc gggcgatatt tgttgcgcac
      601 agaagcatcc aactgggtaa acaattgagc catgcgattt tttatcatct cgatatccgg
      661 agaaccagaa agactttgcg cataatacgg tcctgatgat gttgcataca ttaaagccga
      721 gcccacacct gtcgccatga tgggcgcagc cacggttttg gcaagggcga cgctgcgatg
      781 gctatgcgct tcacaggcta aaggaacata ttccactgac tgcggtaatt cctcacatgc
      841 ctccctcatc attgcggtat gctttacaaa caacggtttt tcactgccca aaaaaacctt
      901 ctgcttaatt gcactaagaa aacgtaaatc caaaatgatt gtttttggtt tattattttt
      961 tggcacattt cctattatcc cgctaagaac cttccttgct gcatggagat ttttttgcac
     1021 agcacataac acaattgacg aggccttctc tactgcgtta ttgattttaa ttttaaaatc
     1081 tgcatgatat acgttgacgg gcaatgcaag tatatcttcc cctacatctt tggctgagta
     1141 tttatttgtg atcgacggtc gtgaatccac gagaaaactt agatgcaaat tatgctctat
     1201 attaccagct gtttttgtag tgtgattaaa catagagatg ccacctctca ttctttcaca
     1261 catatcctca actttttcac ttcgttcttg aggcaatttg gctttaacgt taatttgatg
     1321 atttttaccc gtgcattcct taactacaag aggaatgtta tctaaagtgc catacaggct
     1381 aataacgtca ccaggatttt tatcatgtaa gtaagtttga ttaatactcg aagcattcac
     1441 aaagaggtca atattataac tgtttataac ttcacctaca tcaaatgtgt agtgcttact
     1501 ctgaaggctg ccatccttgt ttaattgtat atcaatcaat atatccccgg actttgtcac
     1561 catatctttg ccataaacca tttgcatagc catttcgagg agatttttat ctaactgatt
     1621 gcgggcgttg agtaagcgac taccaaataa tttatatact gttcccgtca ttgccagcgc
     1681 tgcactttct ggtactttgc gtttccacag tgaggtttct ttatttttaa acaagtcata
     1741 tttattatta ctcgacaacc cgagtttaag ttcgttaaca caatcgcaca gttgctcctc
     1801 accttccatt ttaaggcgat gtgaaaaatc atttaatagc gtttgcttcg gtgattcatt
     1861 actcattagt tgtttttcta aattaacaat agaataactt tcttgtttaa tgagattaat
     1921 attggcaacc attacagcct ccttcccacg gtttaagagc aaacatgcca cataattatt
     1981 tacaacatcc agaagcagta aatgaatgat actgacaatg acgccagtcc attatctttg
     2041 cataatgttt gttagctata gattttaaaa atatttatga gaaaagtatg gcaccagagg
     2101 gggttttgac tatcaaaaat cgctcaaacc tgaactaaga tgatgaatta agcattatca
     2161 gcgataattg ccactcagct ctacaaataa tatttaaatg ctcctgaaca gtgatgtagc
     2221 aagacacgta tggtgccaac gaagaataaa aatttataga gttcttattc aatataaccg
     2281 gaatagtcag agactgaagt aaatgacctg aaagaatgag agaggcagca ccgcccctct
     2341 ccatcagcta ctcttcttcg ttcccgccct cttcatacgg gccaaatggc ttcgcgggca
     2401 gaacgatata cgtgccttca aacactgcac ccggcgtctc gtcgccaaag atttcgacct
     2461 gcatttgtac ccgcgctttg cgcccgcgcg ccagacggtc gagatcgccg cttaaggcac
     2521 cgaggtcggc taccgcatga ggtttaccgc taatcggctt gctgtagcga atatgcgcat
     2581 ccgccagaat aatcgttccg cccagatggc gttcacgcag catcagccag ataagccccc
     2641 aaccggtgag cgtagccagc gagaataaac tcccggcaaa cagcgtatga tgcggattct
     2701 gattgccggt ttccggcgtg gtggtgataa atttttgccc ggtatattgc tgaatgcgca
     2761 cgcccatttt ttcgcttagc gggatatgtt cgtaccacgc ctgctgaagc tgggcgcacc
     2821 agtcgccgcg atgcaaaata tcatccagag tggcgacagg tttaatcatc aaaaaatggc
     2881 gaatcggcgt ggtggttggc gtggtgattt ctccctgatt aacaaacccc agcttggcga
     2941 aaaactccac cgcgtcttcg cgggcgctac aggtcacgcg cttaacacct tcctgacgcg
     3001 ccacagattc gagggtcatc gccatcagcg ttcctaaccc tttgtcctgc acgtcgggat
     3061 gaacggccat aaagcgaata gacgcttcat tgtcggcatt aatatacagt cggcctaccg
     3121 ccaccagatt accctgctcg tcgacgacca tctggtgatg cgccatcgca tcccacgcgt
     3181 cgcgttccga acctttcggt tgatgcaggg gcttacgcaa catttcccag cgaaactgat
     3241 agtaacgctc taattcttct tctgtttgtg gaacccgaag gtgatacata gcgatactct
     3301 ctcttgtttc ccgtgacaac cctggaagct ggctcatacc tgcaaccaga atgtcacggg
     3361 gccatcattg accagcgata cctgcatatc cgcagcgaag cgtcctgttt gcgtgttcat
     3421 ctcttgctga cggcagcgtt cgacgaaata gtcatataac gcctctgcgc gatccggtga
     3481 tgcgcctttg gagaaacttg ggcgcatccc ccgttcggta tctgcggcaa gggtaaactg
     3541 ggaaaccacc agtacactgc cgcccgcctg ttgcacgttg agattcatct tgccttcggc
     3601 atcgctaaag atgcggtagc cgagtacacg ctcgcacaga cggtttgctt tctgttcgtc
     3661 gtcatccttt tcgacaccca ataacaccaa aagtcccgcg ccaatttcac ccgtcacttc
     3721 tccctccacg gtgacgctgg cacgggttac gcgttgaatt aatgcaatca tggttcgtcg
     3781 tcttcttctt gttcagcagc ttgttttaat ttgcggtatt ccccgagagt gacagtaatt
     3841 tccgcgccaa gcaagacgat acaccatgtc cagtagaccc aaacaaagag aatggggatc
     3901 accgccagca cgccgtaaat gagctgatat gacgggaaca tggtgatata aagcgcgaaa
     3961 cctttctttc ctgcttcgaa caggagtgca gcgacaaacg cgccgacaat cgcgtcgcgg
     4021 ttaggtacgc ggatggtagg aacaatgctg tacagcaacc agaaggagat ccacgacaac
     4081 agcagcggaa aaatacgcag cacattgtcg atgacagtat tgagatcgct cgcccagcgc
     4141 agggagagca aataggaact gatcgccaga ctggcccctg ccagcagcgg ccccagcgtt
     4201 aaaatcatcc agtacacggc aaacgagtaa atttttggtc gcgctcgttt actgcgccag
     4261 atggtattca acgcgctatc gatggagtac atcaacaata acgccgtgac gatcagcccg
     4321 cacgccccaa cggcggtcat cttgttggaa ttggcaacaa attgttcgat atattgctgg
     4381 ataacgtcgc cagtggcagg gagaaagttg gcaaaaataa agtgacgcag ttggatacta
     4441 acgtctgaga acatgggaaa agcggcaaaa agcgcaaaaa caacggcaac cagcggcact
     4501 aatgagagca acgacacata ggcaagatta cctgccaggg tggtcatgtt gtcctcatta
     4561 atgcgttgcc agagtagttt cagccaggcc catagcggac gggtacgata cctggctttt
     4621 tcatggatgg tttttagcat aacaccttcg cgaaatagtc cgggatggtg gttttatctt
     4681 tcaccagaat actggtaatg cctagctgat ttgctccttc tatattatcg gcgttatcgt
     4741 cgaaaaagac cgtatcgctg ggggaaaaac cttctgcctg caaaacatgc tggtaaattc
     4801 gcgcttcagg tttgcgcatg cccagatctt gcgaaagata aatatggtca gcagcatcac
     4861 ggatttccgg gtattcctcc ggccagaagg tagtatgcag gcggttggta ttggaaagca
     4921 ccaccacacg atgcccctgc tcacgcagtt tatgcatgat ggcgatcact tccgggcgca
     4981 gcgcgacaaa caccgcctgc cagccgtggg agaactgctc gtagcttagc ggtagagcca
     5041 tctcatgaca catcgcctct gcgaacgctt cgtcgctaat ttccccacgc tcatgctgat
     5101 gaaacgcctc ccccatatga aaactctttt taagcgatgc cagcggaata cgcgttaaat
     5161 cgctccaggt tcccagcaca cggttaaagt cgatatcgac aatcacatta cctaaatcaa
     5221 agatatagag catttttgcc tcctttctcg ccacaagaaa ttaactgtag cgggaaagga
     5281 tgggtttggc tatgtggtca ggtatgaagc aaggtcaacg gtcacaaaat tctacgcgtc
     5341 ttcctggggg aggatgattt caatttggct gtcttgtaag cgtgatttta gcgcagcggg
     5401 tggcagctcg tcggtaaaga gtgcagtgac ctgtgccacg ttaccaattt caactgccgc
     5461 cgaagcatga tacttagtgt ggtcggcgac cagcaggata tttctcgcgt gcgccatcat
     5521 cgttttcact acgttagctt cgttaacatc aaactccatc aacgcgccat cgctctcaat
     5581 cgccccaacg cttgttacaa gataatcagc acgaaaatcg gccacaaagg acgccgctga
     5641 agggccaata atcccgctat tatgcgaacg cagcgtaccg cctggcacca tcacttcaaa
     5701 acgcgggtta tgataaagaa tatgtgccac acgcaggctg ttggtgatta tccgcaaatg
     5761 attatggtta agtaacgccc gggcaacatg ctcaacggtc gtaccgatgg tgataaatat
     5821 tgtcgaacca tcaggaatat aatctgccac cgcctccgcg atggcttttt tttcctcggt
     5881 ttgcgaaacc tcacgctgtt cgaacgccat attaacgacg ctggaggccc gacccgcgcc
     5941 accgtgatgg cgcgtgatca gtccttgctc gcttaattta cgaatatccc ggcggaccgt
     6001 ctgcgtggag acatccagca gatttgccag ctcatcaata ttcatatacc cacgctcggc
     6061 gatcagcatg aggagttggt cgtgccgcgg gttaccggtt agttcggtaa ggctcatgaa
     6121 aattcctcga aaaaccatca ccctggcatt ttatacaaaa agtgacaaaa aagatcgggt
     6181 ttgatcacag tcagggatcc cggctcgtcc accgggacga gtgcatttta acgccgccac
     6241 accgctggcg aagcgaactg actccgctaa attcccaccg gttgccagcg ccaccgccaa
     6301 tgcgccgtga aaaacatcac ccgcacctgt ggtatctacc acatcaactt tgaaggccgg
     6361 ctgatgttga cgcccgccat tttccagcca gtcgcagcct gcgctgccct gggtcacata
     6421 gacatgacca tttgtgagcg tttgcgcctg ttttagcgca ctcgccatct ctttcacgcc
     6481 cgttaagcgc gccagacccg gttctgaaaa agccgcgtga tcgcttaacg ccaccagttc
     6541 gctgatatcc tgcggcgtaa tatccccgtc cagcacagtc atcacgcccg cctgccgggc
     6601 cagagtgaag gcttttttag caccgtcgtg ccagcgtaca tctgccagca caacatccca
     6661 ctgagagaaa tcaatttcct ccaaccactc tgcgtcaggc agcaggtccg ggctggggta
     6721 gttaatgatt atccgctcgc ctttggcatc caccatgatg gcggattgcg aagatttcgc
     6781 ctggttatac cgtttggtgt aacgggtgtt aacaccccag gattccagct ctgccagcag
     6841 gctgttaccc gtgtcgtcat caccgacgcg accaataaaa tcgacctgcg cccccagcct
     6901 tgccgccgca accgctgccg tcgccgctgg cccgccacca acttccgtat aatttctcgc
     6961 cacgtattta ccgctctccg tcggtaatcc ttccacgtaa taaatacgat ccatcacggt
     7021 aatacctaca caagcaacac gaatcatggt cattccttaa gcattttggg atgcgttcat
     7081 tttaatttca ccaattgttt aaaaagtgac ggctgtcaat tttttgacga taaattacaa
     7141 atacgatcaa aaacagacaa gcaaagcatg tcataaatga caaaaagtga catcactgta
     7201 ttcaggagag ggttatggca gcaatcgcgt ttatcggttt aggacaaatg ggttcgccaa
     7261 tggcaagcaa tttattgcag caagggcatc aacttcgcgt ctttgatgtg aacgccgaag
     7321 ctgtgcggca tctggtagac aaaggtgcga ctcccgccgc caacccggcg caggccgcta
     7381 aagatgccga atttatcatt accatgttgc cgaatggcga tctggtgcgc agcgtgttgt
     7441 tcggtgaaaa cggcgtttgc gaaagcttat ctaccgatgc gctggtcatc gatatgtcga
     7501 ccatccatcc gctgcaaacc gataaattga ttgccgatat gcaagccaaa ggcttcagca
     7561 tgatggatgt tccggtaggc cgtacttctg caaatgccat taccggtact ctgttactgc
     7621 tggctggcgg caccgctgaa caagttgaac gcgccacacc gatcctgatg gcgatgggca
     7681 gtgagttgat caacgcgggc ggtccgggca tggggatccg cgttaagctc atcaacaact
     7741 acatgagcat cgcgctcaat gcgctttcgg cagaagccgc tgttttgtgc gaagccctga
     7801 atcttccctt cgatgttgcc gtcaaggtga tgagcggcac cgccgccggt aaaggccact
     7861 tcaccacttc ctggtcaaac aaagtcctca gcggggatct ttctcccgcc ttcatgatcg
     7921 atcttgccca taaggatctt agtatcgccc ttgatgtcgc caaccagctg catgtgccaa
     7981 tgccgctggg ggccgcctca cgggaggttt atagccaggc gcgcgcagcg ggtcgcggtc
     8041 gccaggactg gtccgccatt ctggaacaag tccgtgtcag tgccgggatg actgccaaag
     8101 taaaaatgta acgactggat aaaggaataa atgaatgaat aagtacacca tcaacgacat
     8161 tacacgcgca tcgggcggtt ttgccatgct ggcggtcgat caacgcgaag ccatgcgcat
     8221 gatgtttgcc gcggctgggg cacccgctcc agtagccgat agcgttttaa ctgatttcaa
     8281 agttaacgct gcaaaggccc tctcgcctta tgcctcggcg attctggtgg atcagcaatt
     8341 ctgttaccgc caggtggttg agcaaaacgc gattgccaaa agttgcgcca tgattgtcgc
     8401 cgccgatgag ttcattcctg gcaacggtat tccggtagat agcgtggtca ttgaccgcaa
     8461 aatcaatccg ctacaaatca aacaagacgg tggcaaagcc ttaaaactgc tggtgctgtg
     8521 gcgtagtgat gaagatgcgc agcaacgtct ggatatggtg aaagagttta acgaactgtg
     8581 ccactcacac ggcctggtaa gcattattga gccagtcgtc cgcccaccac gtcgtggcga
     8641 taaattcgat cgcgaacaag cgatcatcga tgctgctaaa gagctgggcg acagtggcgc
     8701 tgacctctac aaagttgaaa tgccgcttta cggtaaaggt ccgcaacaag agcttctcag
     8761 tgcttcacaa cgtctgaatg accatatcaa tatgccgtgg gtgatacttt cttccggtgt
     8821 cgacgaaaaa ctgttcccgc gtgccgtacg cgtggcaatg acagcgggtg catcgggctt
     8881 cctggctggt cgtgccgtct gggcatcggt tgtcggttta ccagacaacg agctgatgct
     8941 gcgtgacgtt tgcgcaccta aattacaaca acttggcgat atcgtcgacg aaatgatggc
     9001 taaacgccgt taagatgggg atacaaaaat gaaatggttt aacaccttaa gtcacaaccg
     9061 ttggctggaa caggaaaccg accgcatctt tgattttggt aaaaattccg tagtaccgac
     9121 cggatttggc tggttaggca ataaagggca aatcaaagaa gagatgggca cccatctgtg
     9181 gatcaccgct cgtatgttgc acgtttattc cgttgctgcg gcgatgggtc gacctggcgc
     9241 ttactcgttg gttgatcacg gtatcaaagc catgaacggc gcactgcgcg ataaaaaata
     9301 tggcggttgg tatgcctgcg tgaatgacga gggcgtggtg gatgcctcca aacagggcta
     9361 tcaacacttc tttgctctgc tgggtgctgc cagcgccgtc actaccggtc acccggaagc
     9421 ccgtaaactt ctcgattacg ccattgaaat tattgagaaa tacttctgga gcgaagaaga
     9481 gcagatgtgc ctggagtcct gggacgaagc cttcagcaaa accgaagagt accgcggcgg
     9541 caatgccaac atgcacgcgg tggaagcctt cctgattgtc tatgacgtca cccatgacaa
     9601 aaaatggctg gatcgcgcga ttcgcgtggc ttccgtgatt atccacgacg tcgccagaaa
     9661 taatcattat cgcgttaacg agcacttcga tacccagtgg aatccgctgc cggattacaa
     9721 caaagataac ccggcgcacc gcttccgcgc attcggtggc acgccgggcc actggatcga
     9781 atggggccgt ttaatgctgc acatccacgc ggccctggaa gcccgttgtg aacagccgcc
     9841 tgcctggctg ctagaagatg ccaaaggtct atttaacgcc accgtgcgcg atgcctgggc
     9901 acccgatggc gcggacggga ttgtttatac cgttgactgg gaaggcaaac cggtggtccg
     9961 cgaacgtgtg cgttggccta tcgtcgaagc gatgggtacg gcttacgcgc tctacaccgc
    10021 caccggcgat cgccagtatg aaacctggta tcagacatgg tgggactact gcattaagta
    10081 cctgatggac tatgaaaatg gttcctggtg gcaggagctg gatgcggaca ataaagtcac
    10141 cacgaaagtc tgggacggca aacaggatat ttatcacctg ctgcattgcc tggtgatccc
    10201 gcgtatcccg ttagcgccag gcctggctcc ggctgttgcc gcaggcctgc tggacgttaa
    10261 cgcgaaataa ctgagctgaa ataacggcat atcaggctac atgccgggta tgtcgttatt
    10321 caataaaggt gcctcgtatg caaatcacca acatgcattg cagtggacaa accgtttcac
    10381 ttgccgcggg ggattatcac gcgactatcg tgacggttgg tgctggtctg gctgaattaa
    10441 cctttcaggg ttgtcattta gtcatcccgc acaaacccga agagatgccg ctggcgcatc
    10501 tgggtaaagt gcttattccg tggccgaacc ggatcgccaa cggctgttat cgctatcagg
    10561 ggcaggacta tcagttaccc attaatgaac atagttcaaa agccgctatt cacgggctgc
    10621 ttgcctggcg tgactggcaa attagcgaat taaccgccac cagcgttacc ctgacagctt
    10681 ttctgccgcc cagttacggc tatccgttta tgctggcttc acaagttgtt tattcgctca
    10741 atgcacgcac gggtctttcc gttgagatcg caagccagaa tattggtact gtgaccgcac
    10801 cttatggtgt gggtattcat ccttatttaa cctgcaactt aacatctgtt gatgactatc
    10861 ttttccagct tccggcagaa caggtttatg ccgtcgatga gcacgcaaat ccgacaacgc
    10921 ttcaccacgt tgatgagctg aatttaaatt tcacgcgggc gacaaaaata gccgcgacaa
    10981 aaatagacca tacctttaaa accgctaatg atctgtggga aataaccatt acccatccac
    11041 aacaggcgct ttcggtgagc ctctgttccg atcaaccctg ggtgcaggtt tatagcggtg
    11101 aaaaattaca gcgtcaggga ctggcagttg aacctatgag ttgcccgcct aacgccttta
    11161 attcaggtat cgacttatta ttactggaac caggaaaaac gcaccgcctg ttttttaata
    11221 ttcacgggca gcacaattaa ttttattaag aaaagaacga gatcattgtt tagggttgta
    11281 tttacacaac aggaaatatt atggactttt ttacgttatt ttcgccggat ggggccatgg
    11341 tggaaacaag cgtggtatgt atcgttatcg cgttaatgtt tctttatacc tttgtgggga
    11401 tatgcgcggg atttggcggt ggtttaacca ctatgccgct cgtcacgtta atgctccccg
    11461 taaaaatggc aactccgctc tcggtgattg tcggcaccgc gaccgccttg tacgccacct
    11521 ggctgtcacg taaagaaacg gattggcgat cggcaatggt actgattatt ttctcgtttc
    11581 tgggaatacc cgtcggttta tatgcgctgt cgtatttacc cgaccatttt atgaaagtgg
    11641 gcttaggcgg attccttatc ctttactcgt tttacagcat gtttattcct cgcctgccga
    11701 tttatgaccg taactggatc gccgcaccta tcggcgcagt agctggcgca ttaggcgcgg
    11761 cgttctcaac caacggaccg ccgatggtta tctacggtat gctgcgtaac ttaggcccgg
    11821 cggcatttcg tggcactctg aacgctttct tcaccgcaaa caacatcgcg gtgatcggcg
    11881 ggctggcgac cagcggaatt ttgactgttt ctatctttaa actggtgctg ttttgtattc
    11941 ccaccatgat cctcggttca ctggttggac aatatgtgca taaacgcatt tcagtcaaag
    12001 tgttccgtgt gatggtcttt ttgttgctga ttgcctccgg ttctatgttg ataaaaggcg
    12061 cgctgggaat tcccgctgct gccgcgcttc tgcctccttt taccttgtta gttatactgc
    12121 aactggcatt tggtaaaaaa gtggcggcaa tccgccgggc tgaagaaagt aaataatcac
    12181 ttatgcgggt tttaatttat acccgcacac attctttaat taatgaaacc tgtaacaaca
    12241 ggatggcatg aaatattagc tttatactct atggagatta tttatggata cgccacgccc
    12301 acaattacca gattttcagt ttcatcagaa taacgactgt tttaccctac attttcaaca
    12361 gcgtcttatt ttaacccata gcaaagataa tccttgttta tggattggtt caggtattgc
    12421 ggatatcgat atgttccgcg gcaatttcag cattaaagat aaactacagg agaaaattgc
    12481 gcttaccgac gccatcgtca gccagtcacc ggatggttgg ttaatccatt tcagccgtgg
    12541 ttctgacatt agcgccacgc tgaatatctc tgccgacgat cagggccgtt tattgctgga
    12601 actacaaaac gacaacctta accacaaccg catctggctg cgccttgccg ctcaaccaga
    12661 ggaccatatc tacggctgcg gcgaacagtt ttcctacttc gatctgcgcg gcaaaccgtt
    12721 cccgctatgg accagtgaac aaggcgttgg tcgcaacaag caaacctatg tcacctggca
    12781 ggccgactgc aaagagaacg cgggcggcga ctattactgg actttcttcc cacagcctac
    12841 gtttgtcagc acgcagaagt attactgcca tgttgataac agttgctaca tgaacttcga
    12901 ctttagcgcc ccggaatacc atgaactggc gctgtgggaa gacaaagcaa cgctgcgttt
    12961 tgaatgtgct gatacataca tctccctgct ggaaaaatta actgccctgc tgggacgcca
    13021 gccagaactg cccgactgga tttatgacgg ggtaacgctc ggcattcagg gcgggacgga
    13081 agtgtgtcag aagaaactgg acaccatgcg taacgcgggt gtgaaggtta acggcatctg
    13141 ggcgcaggac tggtccggga tccgtatgac ctcttttggc aaacgcgtaa tgtggaactg
    13201 gaagtggaac agcgaaaact atccgcaact ggactcacgt attaagcagt ggaacaaaga
    13261 gggcgtgcag ttcctggcct atatcaaccc gtatgtagcc agcgataaag atctctgcga
    13321 agaggcggca aagcgcggtt atctggcaaa agatgcctct ggcggcgact atctggtgga
    13381 gtttggcgag ttctatggcg gcgttgtcga cctcaccaat ccagaagcct acgcctggtt
    13441 caaggaagtg atcaaaaaga acatgattga acttggctgc ggcggctgga tggctgactt
    13501 cggcgagtat ctgcccaccg acacgtacct gcataacggc gtcagcgcgg aaattatgca
    13561 taacgcctgg cctgcgctgt gggcgaaatg taactacgaa gcccttgaag aaacgggcaa
    13621 gctcggcgag atcctcttct ttatgcgcgc cggttctacc ggtagccaga aatactccac
    13681 catgatgtgg gcgggcgacc agaacgtcga ctggagtctc gacgatggcc tggcgtcggt
    13741 tgtcccggcg gcgctgtcgc tggcaatgac cggacatggc ctgcatcaca gcgacattgg
    13801 cggttacacc accctgtttg agatgaagcg cagcaaagag ctgctgctgc gctggtgcga
    13861 tttcagcgcc ttcacgccga tgatgcgcac ccacgaaggt aaccgtcctg gcgacaactg
    13921 gcagtttgac ggcgacgcgg aaaccatcgc ccatttcgcc cgtatgacca ccgtcttcac
    13981 caccctgaaa ccttatctga aagaggccgt cgcgctgaat gcgaagtccg gtctgccggt
    14041 catgcgcccg ctgttcctgc attacgaaga cgacgcgcag acctacaccc tgaaatatca
    14101 gtacctgtta ggccgcgaca ttctggtcgc tccggtgcat gaagaaggcc gtagcgactg
    14161 gacgctctat ctgccggagg ataactgggt ccatgcctgg acgggtgaag cattccgggg
    14221 cggggaagtg accgttaatg cgcccatcgg caagccgccg gtcttttatc gcgccgatag
    14281 cgaatgggcg gcactgttcg cgtcgttaaa aagcatctaa tcacgtccgc ccgtggggaa
    14341 actcacgggc aaagggagaa caacgatgag tcacatcaca acggaagatc cggctacttt
    14401 acgtctgccc tttaaagaga aactctctta cggtatcggc gatctggcct ctaacatcct
    14461 gctggatatc ggcacgcttt atcttttgaa gttttatacc gacgttctgg ggctgcccgg
    14521 cacctatggc ggcattatct ttttgatctc gaaattcttt accgccttta ccgatatggg
    14581 taccggcatc atgctcgatt cccggcgcaa gatcggtcca aaaggcaaat tccgcccttt
    14641 tattctgtat gcatcattcc cggtcactct gctggcgatc gccaactttg tcggcacacc
    14701 gtttgatgtc accggtaaaa cggtgatggc cactattctg tttatgctct acgggctgtt
    14761 tttcagcatg atgaactgct cctacggcgc aatggtcccc gctattacca aaaaccccaa
    14821 cgaacgcgca tcgctggcag catggcgtca gggtggcgct acattgggct tactgctgtg
    14881 tacggtggga ttcgtgccgg ttatgaatct tatcgaaggt aatcagcaaa ttggctatat
    14941 cttcgctgcc acgctgtttt cactgttcgg cctgctgttt atgtggatct gctactcggg
    15001 cgtgaaagag cgttatgtcg aaacccaacc taccaatccg gcgcaaaagc caggcctgtt
    15061 gcaatctttc cgcgcaattg ccggtaaccg cccgctgttc attctgtgca ttgccaacct
    15121 ctgcacttta ggggcgttta acgtcaagct cgccatccag gtctattaca cccagtacgt
    15181 acttaacgat cccatcctgt tgtcgtatat gggatttttc agcatgggct gtattttcat
    15241 cggcgtgttc ctgatgcctg gcgcagtcag acgttttggt aagaagaagg tctatatcgg
    15301 cggcctgctg atttgggtgc tgggcgatct gctcaactat ttcttcggcg gcggctcggt
    15361 cagctttgtg gcgttctcct gcctggcatt cttcggctcg gcgtttgtta acagcctgaa
    15421 ctgggcgctg gtttccgaca ccgtcgagta cggcgagtgg cgcaccggtg tgcgttcgga
    15481 aggtacggtc tacaccggct tcaccttctt tcgcaaagta tctcaggcgc tggcaggttt
    15541 cttcccaggc tggatgctga cgcaaatcgg ctatgtgcca aacgtcgcac aggctgacca
    15601 caccattgaa gggttgcgcc agctgatctt catctaccca agcgcactgg cagtagtcac
    15661 cattgtggcg atgggttgct tctacagtct gaacgagaag atgtacgtcc gcattgttga
    15721 agagatagaa gcccgtaaac gcacggcgta attataataa acaacgccct gcggggcgtt
    15781 ataaggagtg attatgtctg accataatcc actgacatta agactgaatc tgcgggaaaa
    15841 aatcgcctat ggtatgggcg acgtcggttc gaatttaatg ctctgcatcg gtactctgta
    15901 tctcctcaaa ttttataccg atgaattagg gatgcctgct tactatggcg ggattatctt
    15961 tctggtggcg aagtttttta ccgcgtttac cgatatgctc accggatttt tactcgactc
    16021 gcggaaaaat attgggccta aaggcaaatt tcgtcccttt attttatatg cagcggttcc
    16081 ggcggcgtta attgcaacgc tccagtttat cgccaccacc tttagtttgc cggttaaaac
    16141 gacgattgcc accgcactgt ttatgatgtt tggactttca tacagtctga tgaactgctc
    16201 gtatggtgcg atgatcccgg caattaccaa aaacccgaat gagcgcgcgc agctcgcggc
    16261 ttaccgccag ggtggtgcaa ccatagggct attgatttgt accgtggcgt ttattccgtt
    16321 gcagtcgctt ttttctgact ccaccgtcgg ttatgcctgc gcggcactga tgttctccat
    16381 tggtggcttt atttttatga tgctgtgcta cagaggcgtc aaagagcatt atgtggacac
    16441 gacgccaacc ggacataaag ccagtattct caaatctttt tgcgcgatat ttcgtaatcc
    16501 gccattgctg gttttgtgca ttgctaacct gtgtaccctg gcggcattta atatcaaact
    16561 ggcgattcag gtctattaca cccagtatgt gctgaatgat attaatttat tgtcgtggat
    16621 gggatttttc agtatgggat gtatcctcgt cggcgtatta ctggtgccat taactgtaaa
    16681 atgttttggt aaaaaacagg tttatttagc tggcatggtg ctatgggcgg ttggtgatat
    16741 cctgaattat ttctggggaa gtaactcttt cactttcgtc atgttctctt gtgtcgcctt
    16801 ttttggcacg gcctttgtaa acagcctgaa ctgggcactg gtgccagata ccgtagatta
    16861 cggtgaatgg aaaaccggta ttcgtgccga aggttctgtt tataccggtt ataccttctt
    16921 tcgtaaaatt tctgccgcac ttgctggctt cttgccaggc attatgctga cgcaaatcgg
    16981 ttatgttccc aacatcgcgc aaagtgatgc gacattacag ggtttgcgtc agctcatttt
    17041 tatctggcct tgtgcactgg caattattgc tgcattgacg atgggattct tttacacact
    17101 caatgaaaaa cggtttgcat tgattattga ggaaatcaac caacgcaaaa ataaagagat
    17161 ggcgacagaa gaaaaaacgg cttccgtaac gttataaatg tcaatagccg ctatttccat
    17221 cctggtgggt ggcggcctcc ctacgtttaa aaatggactt attatgaaaa agattaatgc
    17281 aataattcta ttatcctctc tgacctctgc ctcggtattt gctggcgcat acgtcgaaaa
    17341 cagggaggca tataatctcg cctccgatca gggcgaagtt atgctacgcg tgggttataa
    17401 cttcgatatg ggcgcgggta ttatgttaac caatacctac acctttcagc gagaagatga
    17461 actcaaacat ggctataatg aaattgaagg ttggtatcct ttatttaaac caaccgataa
    17521 attaaccatc cagcccggtg gcttaattaa tgacaagagt atcggttcgg gtggtgccgt
    17581 ttatctggat gttaactaca aatttactcc gtggtttaac ctgacggtgc gtaatcgcta
    17641 taaccataat aattacagct ctacagattt gaacggcgaa ttggataata acgacaccta
    17701 tgaagttggc acctactgga attttaaaat caccgagaaa ttctcctata catttgaacc
    17761 acattacttc atgcgagtaa atgactttaa tagcagcaat ggaaaagatc atcactggga
    17821 aatcactaac accttccgtt atcgcattaa tgaacactgg ctcccttatt tcgaattacg
    17881 ctggttagat cgtaatgtcg aaccgtacca ccgcgagcaa aaccagattc gtatcgggac
    17941 gaagtatttc ttctgatgta aaactgccgg atgcggcgaa aacgccttat ccggcctacg
    18001 gttgatacgg tttccgttgg ccggattaag ccacatccgg ccattaatca agcttcagcc
    18061 gccagcatct ctttcttacg ttggctaata cgcttaacca gcattacgga aaccaccgca
    18121 ccacagaatg cgaagcaggc cataatgcca aacacaattt tgtagccaat aatccccgga
    18181 tttaaatcca gaatgtagcc atacagactg aagcagaaca tcgccggggc gtaaccaata
    18241 aagctaccca acgccatcgc cgcgcctgtt ttattttcag caatttttgc ttcgccgata
    18301 ggtgcaaaaa atacggctcg ctgtgtaaag actatcgcgc caaagcccag cgtacatgcc
    18361 atccctaaat ataccggcat actttcgtgc ggcagcataa tcaacagtac gagcgcagcg
    18421 gtactgatga taaaggtgta gcatagatat ttactcggcg atttcaggat cttatctgaa
    18481 atcatgccac caatcggtcc accaatcatt ttcagacagt attggttaat gatgccgtaa
    18541 gcccccacca gcgcaacggg caatagatag atgtttttca ggaatggaat gaagaatgtc
    18601 aggccacagt aaaccgcgta aacgaagaag acgttgaaag cgataagcca gatagttttg
    18661 tctttcagca ccgaggtcat actggtgttt ttcgatgctc cgtcttcttt tttcacctca
    18721 acggacggtg cctcttcttt gtcattcaga acaaagaaaa tgataatgcc tacggcaatc
    18781 acaataaggg aatagaacca gatgcctgct ttaaaaccca ataagccact gccaaaccag
    18841 gtaaatactg ccaacgcaga aaatgccacc acggtatcga caatgccacg ccctgtttcg
    18901 aagaagccaa acaaccgacc ttgttgttcg ctattgccca atcgacttac cgacttgagc
    18961 aagaccggcc agttcatcat gtcgcaagta acgccaaata gcgcccagac aaagaggatg
    19021 ccccaatacc ccggcattgt cgtcaaataa acacccagta atcctgtcgc aatgagtgag
    19081 aatgacatgg tgtatctgcg cggtagttta tcggcaaaat aaatagacag aaaaaagccc
    19141 actgtcgtga caaatgagtt taccgacata gcattaccaa tttgaccatt ggtcaaatgg
    19201 aaatattcct gcatcgggat ataaaacgca tctttcagcg acggtaattt ataaattgta
    19261 ccgccacaca atgttaatag actaaataac gcccatttct ttttcgtgag cataacatcc
    19321 cctaagcgat ttttaaaatc actgttttaa aggcaataag ataccgacca ttcatatcga
    19381 atggttttat taactgcgta tgagttaaaa cttattagtg gttcagaaga tggtttgcct
    19441 cttgtttgat ttgttgtagt acgttacgca catgatttat ctgattaatt gcatcttctt
    19501 tttctttacg taaccgcgca tccagtaaat gattttctgg caatggtgtt tcactcatct
    19561 ggcgataaga gatccacgga ttatcatctt cgtcttcaaa gcggaacgcc ggcgcatagt
    19621 aatcaacctc ttcttccagg ccatatgccg tcacctgcgg ctcatcatca cccaggcata
    19681 taaggtgcgt taataacgct ttgaacggca tatcaccctg acctgaaata caggctttat
    19741 gacccaggcc accctcttct ttaacgatca aggcatcttt gatatggacc tgggtaatat
    19801 gcggtgccat cgtttttaaa gcgtcgatgg gatgctcatt tgcattgatc atgttcgcaa
    19861 aatcaaacag taaggaaaga gattccatct cactttcttt gaccagcgac accaactcat
    19921 ggctttttaa atcttcatgc tgttcgatcg taaaagtcag gccgctgtcc tgatacgttt
    19981 cccgtacata ggcaatgtcg ttagcgatga tcgataatac gtcgcgcaaa ttaccttcat
    20041 aacgtggata aaaacgtacg gacgatgccc cagttttcaa cgcaatggcg acggcttcgt
    20101 caatagatgc cttatctgaa gcgctggttt caatatgaat atcaaggttc agacggcggg
    20161 ctttatcccc aaaggcagag agttctttat cgtccatatt accaagagaa aaacgctcgc
    20221 cgtccagaac atggattttg acgccacgta gattattttc acttgcgata tcaagtaaat
    20281 cagcaggcaa cactctttcc agccgcatat ttaaatgaaa ggcataagca tgtaaataaa
    20341 gaggaagagt gtcgacacgt tgtagaatct ttcttgcgtt atttattgtc accataaaac
    20401 acctacagat taatgatgag gtctacggcc tcaaatcgcc aatattcaat atctatctgg
    20461 acaatattgc cttgctggtc ggcgcgatgc ttttcaatta agatcgctgg catacccgca
    20521 gaaccgccaa gatacttgct gatatcgccc ggcattctga cgggtttgaa aatcaatttt
    20581 tttactaccg tctctttctg gtagcacttt tcccagactg cagaaaatga gtggttttca
    20641 agttgttcaa taaaacccgg agcaacttca ggattaatat atgtttcgtg atagaaaact
    20701 ttatgtcctt ccagcgcccc ccagccggtg atgcgataga gttcagttga tggtgtcact
    20761 gcaatcagtg gcgcgagcgt ctcgggaata tcgctggtac ggtttttctc ggtaaacccc
    20821 caagaaggct ctcgtccttg ctcaattgcg gcccgctgaa agctcgctga cagctccgga
    20881 ctgtaattaa atcgcggctg ggtcacgaac caccccttac gatccttacg gaagattttg
    20941 gattctgcct cgaggttcaa caacgcctgg cgcagcgtca tacgtttaat gcccagcagt
    21001 tctcccagct ctctttctga agggagtttt ccacccggcg tggtgatgcc atctttcaac
    21061 caccgatcca gtttctcttt tgcattttct acggtggatt gattctccgc cattgtttaa
    21121 tccttttggt ttaaaccaga tgaagcatct tacgcttacg ccaaaaaaga aagcaattaa
    21181 cggtgtatga ctttgcagat aattgtgaca aagctctcta ttgacgtaat cacagactta
    21241 taagggagat actcgcattg gtgtgcgctc acgacaggta aaaaaaaacc tgccagcgat
    21301 ggcaggtttt ttataactaa aaaacgaaat taatcgtctt tcggtgcgcg gttggcgcga
    21361 cgacgatcgt tttccgtcag gtgacgttta cgaatacgga tagaggtcgg agtcacttct
    21421 accagttcgt catcatcgat gaactccaga gcttgttcca gagtcatgcg gataggcgga
    21481 accagaacaa cggcttcgtc agtaccggaa gcacgcatgt tggtcagttt cttaccggtc
    21541 aggcagttta cagtcaggtc gttagagcgg ctatgaatac cgataatctg accttcgtaa
    21601 acttctgcac cgtgaccgag gaacagctta ccgcgatcct gcagaccgaa cagcgcgaac
    21661 gctaccgctt taccctgacc gttagagatc agtacgccat tctgacgctg acccacttca
    21721 cccggacgta cgtcgtcgta gtggctgaag gtggagtaca gcagacccgt accggaagtc
    21781 atggtcatga actcagaacg gaaaccaatc agaccacggc ttgggatcac gtagtcgaga
    21841 cgtacgcggc ctttaccgtc tggattcatg tttttcaggt cgcctttacg ttcgcccagc
    21901 gcctgcatta cagaaccctg atgctgttct tcaacgtcca gagtcacgtt ttcatacggc
    21961 tcttgtttac gaccgtcgat ttcacggaag ataactttcg gacgggatac cgccagttcg
    22021 aaaccttcac gacgcatgtt ttcgatcaga acagacaggt gcagttcgcc acgaccagaa
    22081 acgcggaacg catcggcgtc ttcggtttct tctacgcgca gagcaacgtt gtgtaccagt
    22141 tctttgttca gacgatccag gatctgacga gacgttacga acttaccttc tttaccgcag
    22201 aacggcgagg tgttaacgca gaagaacata gaaacggtcg gctcatcaac ggagagtgcc
    22261 ggcagcgctt caacgttttg cgtgtcgcaa acggtgtcag aaatgttcag ttcgcccagg
    22321 cccgtgatcg ccacgatatc gccagcttcc gccaggtcgg tttcgatacg ttccagaccg
    22381 aggtggccca gcactttacc gactttcgcg ttacgggttt tgccttcgct atcgatgata
    22441 gtgacctgct ggttcggctt cactttaccg cgcttgatgc ggccaatgcc gataacgcca
    22501 acatagctgt tgtaatcgag ctgagaaatc tgcatctgga acggaccgtc aaggtcaacg
    22561 tccggcgcag gaacgtggtc aacaatcgcc tgatacagcg gggtcatgtc ttccgccata
    22621 tcttcgtggt ccagacccgc gataccgttc agcgcagaag cgtaaacgat cgggaagtcc
    22681 agctgctcgt cggtcgcgtc gaggttaacg aacaggtcga atacctgatc cacaacccag
    22741 tcaggacgcg cgccagggcg gtcaactttg ttgataacaa caatcggctt caggccgtaa
    22801 gcaaacgctt ttttggttac gaagcgcgtt tgcggcatcg ggccgtcaaa tgcgtcaacc
    22861 accagcagca ctgagtctac catggacatt acacgttcaa cttcaccacc gaagtcggcg
    22921 tgccccgggg tatcaacgat gttgatacgg taatcattcc atttgatagc ggtgtttttc
    22981 gcgaggatgg taatcccacg ctctttctcc aaatcgttgg agtccatcac gcgctcttgg
    23041 gtttcggcac gagagtcgaa cgtaccggat tgttggagca gcttgtctac cagggtggtt
    23101 ttaccatggt ctacgtgcgc gatgatggcg atattacgca atttttcgat cacaactttg
    23161 cctcaggcat tagaaatagc gcgttattgt acacggatta atcgcactac aaaacaggat
    23221 cacaaacatc ctccgcaaac aagtattgca gagtcccttt gtgattgctt tcacggagca
    23281 taaaaagggt tatccaaagg tcattgcacc aatatggtgc ttaatgtttc cattgaagca
    23341 ctatattggt gcaacattca catcatggtg cagccctttt gcacgatgtt aagcatgata
    23401 acgcctttta ggggcaattt aaaagttggc acagatttcg ctttatcttt tttacggcga
    23461 cacggccaaa ataattgcag atttcgttac cacgacgacc atgaccaatc caggagagtt
    23521 aaagtatgtc cgctgaacac gtactgacga tgctgaacga gcacgaagtg aagtttgttg
    23581 atttgcgctt caccgatact aaaggtaaag aacagcacgt cactatccct gctcatcagg
    23641 tgaatgctga attcttcgaa gaaggcaaaa tgtttgacgg ctcctcgatt ggcggctgga
    23701 aaggcattaa cgagtccgac atggtgctga tgccagacgc atccaccgca gtgattgacc
    23761 cgttcttcgc cgactccacc ctgattatcc gttgcgacat ccttgaacct ggcaccctgc
    23821 aaggctatga ccgtgacccg cgctccattg cgaagcgcgc cgaagattac ctgcgttcca
    23881 ctggcattgc cgacaccgta ctgttcgggc cagaacctga attcttcctg ttcgatgaca
    23941 tccgtttcgg atcatctatc tccggttctc acgttgctat cgacgatatc gaaggcgcat
    24001 ggaactcctc cacccaatac gaaggtggta acaaaggtca ccgtccggca gtgaaaggcg
    24061 gttacttccc ggttccgccg gtagactctg ctcaggatat tcgttctgaa atgtgtctgg
    24121 tgatggaaca gatgggcctg gtggttgaag cccatcacca cgaagtagcg actgctggtc
    24181 agaacgaagt ggctacccgc ttcaatacca tgaccaaaaa agctgacgaa attcagatct
    24241 acaaatatgt tgtgcacaac gtagcgcacc gcttcggtaa aaccgcgacc tttatgccaa
    24301 aaccgatgtt cggtgataac ggctccggta tgcactgcca catgtcgctg tctaaaaacg
    24361 gcgtcaacct gttcgcaggc gacaaatacg caggtctgtc tgagcaggcg ctgtactata
    24421 ttggcggcgt aatcaaacac gcgaaagcga ttaatgccct ggcaaacccg accaccaact
    24481 cttacaagcg tctggtcccg ggctacgaag caccggtaat gctggcttac tctgcacgca
    24541 accgttctgc gtctatccgt attccggtgg tttcttctcc gaaagcacgt cgtatcgaag
    24601 tacgtttccc ggacccggcg gctaacccgt acctgtgctt tgctgccctg ctgatggccg
    24661 gtcttgacgg tatcaagaac aagatccatc cgggcgaagc gatggacaaa aacctgtatg
    24721 acctgccgcc ggaagaagcg aaagagatcc cacaggttgc tggttctctg gaagaagcac
    24781 tgaacgaact ggatctggac cgcgagttcc tgaaagccgg tggtgtgttc actgatgaag
    24841 cgatcgatgc gtacatcgct ctgcgtcgcg aagaagatga ccgcgtgcgt atgactccgc
    24901 atccggtaga gtttgagctg tactacagcg tctaatagtt gaagttgtac tacccggcgc
    24961 aacaacgccg ggatttagtt gccgtggaaa ctttcagccc atctctgcat gggctttttt
    25021 ctccgtcaat tctctgatgc tacgcgcttt ttatccgtaa aaagctataa tgcactaaaa
    25081 tggtgcaacc tgttcaggag actgctttat ggcaacaggc acgcagcccg atgctgggca
    25141 gatcctcaac tcgctgatta acagtatttt gttaatcgat gacaacctgg cgatccatta
    25201 cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc cgcaaattgt ttggtacacc
    25261 gttaccggaa ctattgagct acttctcatt aaatatcgag ctgatgcaag aaagtctgga
    25321 ggcggggcaa ggttttaccg ataacgaagt gacgctggtc atcgacgggc gctcgcatat
    25381 cctttctgtg acggcccagc gtatgccgga cggcatgatc ctgctggaga tggcaccgat
    25441 ggataaccag cgccgcttaa gtcaggaaca gctacagcac gcccagcagg ttgctgcccg
    25501 tgatttagtg cgcggcctgg cacatgagat taaaaatccg cttggcggtt tacgtggcgc
    25561 ggcgcagcta ctcagcaaag cattacctga cccgtcacta ctcgaatata ccaaagtgat
    25621 tatcgaacag gcggaccggc tgcgaaatct ggtcgaccgt ctgttgggac cgcaactgcc
    25681 cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa cgcgtggtga cgttggtgtc
    25741 gatggaactg ccggacaacg tgcggttgat tcgtgattac gatcccagcc tgccggaact
    25801 ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat attgtgcgca atgcgctaca
    25861 ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc cgcaccgcgt ttcaactgac
    25921 cttacacggc gagcgctatc ggctggcggc gcgaattgat gtggaagata acgggccggg
    25981 cattccgcct catttgcagg atacgctgtt ttatccgatg gtcagtggcc gcgaaggtgg
    26041 caccgggctt ggcttatcca tcgcccgtaa tttgattgat cagcattcag gcaaaattga
    26101 atttaccagt tggccaggtc ataccgagtt ctcggtttac ctgcctatca ggaaataaag
    26161 gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg acgatagttc catccgttgg
    26221 gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta caacatttga gaacggcgcg
    26281 gaggtactgg aggcgctggc gagcaaaacg ccggatgtgc tgctttcaga tatccgtatg
    26341 ccgggaatgg acgggctggc gctgctcaag cagattaaac agcgccatcc gatgcttccg
    26401 gtcatcatta tgaccgcaca ttccgatctg gatgctgccg tcagcgccta tcaacaaggg
    26461 gcgtttgatt atctgcccaa accgtttgat atcgacgaag ccgtcgccct ggttgagcgc
    26521 gccatcagtc attaccagga acagcagcag ccgcgtaatg ttcagcttaa cggcccaacg
    26581 accgatatca tcggcgaagc gccagccatg caggacgtgt tccgtattat cggtcggctt
    26641 tcgcgttctt ctattagcgt gctgattaac ggcgaatccg gcaccggtaa agagctggtc
    26701 gctcatgccc tgcatcgcca cagtccgcga gccaaagcgc cgtttatcgc gctgaatatg
    26761 gcggctatcc cgaaggattt gatcgaatca gaactgtttg gtcacgagaa aggcgcgttt
    26821 accggcgcga ataccattcg tcaggggcgt tttgaacagg ccgatggcgg cacgttattt
    26881 ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc gtttgctgcg cgtgctggca
    26941 gacggtcagt tttatcgcgt tggcggctat gcgccggtga aagtggatgt gcggattatc
    27001 gctgccactc accagaatct tgaacagcgg gtgcaggaag gtaagttccg tgaggatctg
    27061 ttccaccgcc tgaacgtcat ccgcgttcat ctgccaccac tgcgtgagcg tcgggaagat
    27121 attccccgcc tggcacgcca ttttttacag gttgccgcgc gcgaactggg cgtagaagcg
    27181 aagttactgc atccggaaac cgaagccgcg ctgacgcgtc tggcgtggcc aggcaacgtg
    27241 cgccagctgg aaaacacctg ccgctggcta acggtaatgg ctgccggaca ggaagtgttg
    27301 attcaggatt tgcctggcga actgtttgaa tcaacggttg cggagagtac ttcgcaaatg
    27361 caaccggaca gttgggcgac gctgttagcg cagtgggcag acagggcgct gcgttccggt
    27421 catcaaaatc tgctttccga agcgcagcct gagctggagc ggacgttact gaccaccgcg
    27481 ctgcgacata cgcaggggca taaacaggaa gcggcgcggc tactcggctg ggggcgtaac
    27541 actctgacgc ggaagttaaa agagctgggg atggagtgat tcacggcttg tgtgtaaaga
    27601 ttgattattg agcgcaaatt gctggtattt tgcgctttac tgttccgata agttcagtat
    27661 gatcttgccc ggaaaacggg gagagtcatt atgctggaat caataattaa tctggtatcg
    27721 agtggcgcag ttgacagcca cacaccgcaa actgccgttg ctgccgtgct gtgtgccgcg
    27781 atgattgggc tgtttagctg agtttcagat tgctgatgaa gtgctctttg ttcatgccgg
    27841 atgcggcgtg aacgccttat ccggtctaca aaagcaatat tttcaacagg ttttgtaggc
    27901 ctgataagcg tagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt
    27961 ctactttgta aacgaagcgc cattcactat ttaaatcacc cgtgagaact gctgcatccg
    28021 cgctttctgg cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc
    28081 tttcgccgtc acctgaatcc ccttctcatc cacatccacc agcccatctt ttgctaacgg
    28141 ggcgagcagc ttgagatctt cggcaaagta atcagcgaag tgcaaatccc actgttgctc
    28201 aatgggagcg taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat
    28261 acagtcatca cgcgttagcg caataccacg ccacagcgca ttgccttgtt catccacttg
    28321 ctgatagtac tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat
    28381 ggcggaaacg cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt
    28441 acgatgcagc acgccttcac gctgggccac cgccagttcg tcatccggac gggcaaagtg
    28501 atccataccg ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat
    28561 atcgagtttt tgctgcggac tcggcaagtc agcatctttg attttgcgct gagcagcaaa
    28621 aatggtcggc agatgcgcat agttaaagac gctcagacga tcggggttca gctccgccac
    28681 gcgtttcagg gtaaaagcga aactctccgg cgtttgtttc ggcaggccgt aaatcaggtc
    28741 gatgttggtg gaggtaaagc caatctcacg cgcatggtta agcagtgcaa agatgaactc
    28801 ttcatcctgc tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat
    28861 gctcaggcga ttaaagccct cggcgcgtaa atgatcgagt acatccagtt cgatttcgcg
    28921 cggatcgact tcgatcgaaa tctccgcatc ggcattgaac tggaagtttt cgcgcagcag
    28981 cttcatcaga cggctgattt gcgctttgtt cagatacgtc ggcgtaccac cgccccagtg
    29041 cagctggctc accttgcgcc cggcaaaaag cggtgcacga tggacgattt cttgctccag
    29101 cgcgtccaga tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca
    29161 gaagtaacaa agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg
    29221 atagcgcgcc acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc
    29281 ggtcgggtac gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc
    29341 ccagtcgatt tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg
    29401 cgtaccggcg gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc
    29461 gacaaccgct gtagtttaac gaataaccac accagataac atataaccgg aagggttatc
    29521 agcagaacgg gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg
    29581 tcttcttgct tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc
    29641 tgcatcaact catcaatacg atccagtttg gcatccaccc aggattgctc ttcggcgctc
    29701 agggtttcgc ctgcctccag acgttccagc agcgcatcca gacgctcatc cgtttccagt
    29761 aactccaact ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg
    29821 gtgacttttt cagtcacgcc caatggaata ggggttttac tgccaatacg tggatctttc
    29881 ggtgcgttct ggcctttgct gcctgacgtg gtgttaccgc ccgctgcgcg gctgcccggc
    29941 gcatgaccac gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg
    30001 cgtgtttttc gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc
    30061 ataatggttc gtcttttagc cgttttattc agtatagatt tgcggcggaa t
//
LOCUS       DAPOZO010000061        29083 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPOZO010000061 DAPOZO010000000
VERSION     DAPOZO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.061"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1161)
                     /gene="lplT"
                     /locus_tag="ROI21_002912"
     CDS             complement(<1..1161)
                     /gene="lplT"
                     /locus_tag="ROI21_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDX5111813.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAI"
     gene            complement(1154..3313)
                     /gene="aas"
                     /locus_tag="ROI21_002913"
     CDS             complement(1154..3313)
                     /gene="aas"
                     /locus_tag="ROI21_002913"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDX5111814.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGEHIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            3899..4930
                     /gene="galR"
                     /locus_tag="ROI21_002914"
     CDS             3899..4930
                     /gene="galR"
                     /locus_tag="ROI21_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDX5111815.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(4937..6199)
                     /gene="lysA"
                     /locus_tag="ROI21_002915"
     CDS             complement(4937..6199)
                     /gene="lysA"
                     /locus_tag="ROI21_002915"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDX5111816.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTYPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            6321..7256
                     /gene="lysR"
                     /locus_tag="ROI21_002916"
     CDS             6321..7256
                     /gene="lysR"
                     /locus_tag="ROI21_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDX5111817.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAAKEILTPEDFHSENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(7243..7935)
                     /gene="ygeA"
                     /locus_tag="ROI21_002917"
     CDS             complement(7243..7935)
                     /gene="ygeA"
                     /locus_tag="ROI21_002917"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDX5111818.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAIIRAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(8064..9482)
                     /gene="araE"
                     /locus_tag="ROI21_002918"
                     /pseudo
     CDS             complement(8064..9482)
                     /gene="araE"
                     /locus_tag="ROI21_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(9797..10558)
                     /gene="kduD"
                     /locus_tag="ROI21_002919"
     CDS             complement(9797..10558)
                     /gene="kduD"
                     /locus_tag="ROI21_002919"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDX5111819.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(10588..11424)
                     /gene="kduI"
                     /locus_tag="ROI21_002920"
     CDS             complement(10588..11424)
                     /gene="kduI"
                     /locus_tag="ROI21_002920"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDX5111820.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFIADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(11711..12892)
                     /locus_tag="ROI21_002921"
     CDS             complement(11711..12892)
                     /locus_tag="ROI21_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDX5111821.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVS
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            13147..14376
                     /locus_tag="ROI21_002922"
     CDS             13147..14376
                     /locus_tag="ROI21_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDX5111822.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSVGN
                     LITLIVGILCVSVMFFS"
     gene            14836..15468
                     /locus_tag="ROI21_002923"
     CDS             14836..15468
                     /locus_tag="ROI21_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX5111823.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            15802..16611
                     /locus_tag="ROI21_002924"
     CDS             15802..16611
                     /locus_tag="ROI21_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDX5111824.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHAINSSTDAHTHNAPPAVKGNAGYKESIGSANNFNNKILKHIKSYLIML
                     SIFAIGVYSAHWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRD"
     gene            16604..17086
                     /locus_tag="ROI21_002925"
     CDS             16604..17086
                     /locus_tag="ROI21_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111825.1"
                     /translation="MIDYKKNLLFILVFISGFILFIVYSYTAEKMIYNETCTANWVIF
                     NDKGRANLTIDFMYNQKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAELIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(17119..17199)
                     /locus_tag="ROI21_002926"
     CDS             complement(17119..17199)
                     /locus_tag="ROI21_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111826.1"
                     /translation="MKDVDQNFDALYWHILQAYLILLFYD"
     gene            complement(17235..17660)
                     /locus_tag="ROI21_002927"
     CDS             complement(17235..17660)
                     /locus_tag="ROI21_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111827.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWVLNSRYVNVNSVSKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            18090..18581
                     /locus_tag="ROI21_002928"
     CDS             18090..18581
                     /locus_tag="ROI21_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111828.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKYVNPVKITMRSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYKFPITDDLMSMATNELIKLA
                     KIENNLDLNKFSTICTDVLSPLVTRHNKEKKPDDLLPFAKIPFLIFIEKMEIDNMNPG
                     HAH"
     gene            18807..19298
                     /locus_tag="ROI21_002929"
     CDS             18807..19298
                     /locus_tag="ROI21_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDX5111829.1"
                     /translation="MSTDTLEIFNNSDEWASQLKHALSQGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKGFASVCQSKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDESVKSKAQAYIELLTDNSE
                     DNG"
     gene            19633..21009
                     /gene="ygeH"
                     /locus_tag="ROI21_002930"
     CDS             19633..21009
                     /gene="ygeH"
                     /locus_tag="ROI21_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HDX5111830.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQIYIPPKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYARCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQNISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLRRFKPDYFVTGRINQNNAVKTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHIPERSVRLAKPDQGYKNHYLSDELLAGKKELYEFTPESIYR
                     AMAIFDGLQNKSDIQTLKIECYCLLAECHMSLALHGKYELELAAQKALELLHYVSDIT
                     NVDGKILAIMGLITGLSGQAKVSHILFEQAKIHTTDIASLYYYRALVNFHNERIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKSNMKLYYKETSSESHRVIIDNILKLK
                     QLTRICMR"
     gene            21176..21394
                     /locus_tag="ROI21_002931"
     CDS             21176..21394
                     /locus_tag="ROI21_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111831.1"
                     /translation="MTNPINMNNFSQSLNIATATGDEVVSLDKHITTSATDTDQIQAF
                     IVSTWMASFQNDMYSKDNPIFPYYELEW"
     gene            21478..21981
                     /locus_tag="ROI21_002932"
     CDS             21478..21981
                     /locus_tag="ROI21_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDX5111832.1"
                     /translation="MRLPKVKILAIFMFSILITKMSFASSACFNEAGSIFRIEPNLIK
                     AIALVESNLKNDSIGKNRDKKNNIKSFDYGLMQINQMHIPMLKKRGIIKDEQDLLDNP
                     CLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFAMENQKKRLQYAKKIYVVYTRLNEID
                     KRKALAK"
     gene            complement(22001..22633)
                     /locus_tag="ROI21_002933"
     CDS             complement(22001..22633)
                     /locus_tag="ROI21_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDX5111833.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGSELIKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
     gene            complement(22856..23563)
                     /locus_tag="ROI21_002934"
     CDS             complement(22856..23563)
                     /locus_tag="ROI21_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111834.1"
                     /translation="MRKKIEMSLINSPANGVVIKRIISDGLKEIVSLKEQILLETTAK
                     IQSIEEKREEKVIQGYYDGYAKGIIDVMDNFIPLISLLSSELEKNRINMINDLKSILL
                     KSSEEVEVFIKIFESWVTKLPSISGPLNLYIPTSFKDKSIEVESYFVDKSIWNVHISY
                     HDDKRFVFFTDQFIAEFSPQEFVDNCEQYLISNHCFSPDKVNEICEHARHYLVERMCE
                     IDSLAMNNSDLTTPEDL"
     gene            complement(23505..24086)
                     /locus_tag="ROI21_002935"
     CDS             complement(23505..24086)
                     /locus_tag="ROI21_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HDX5111835.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNARISNPVLRSITNEMILLQ
                     YNLSVEHFSLNSSLIYYINNWKLFPLICLLSGCHFYRERFAERGFFYKVPDVLRNYLS
                     AIPVEINEKARYKPGIVNYQNIITCGFSTLLPYVRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKLSCKWCCD"
     gene            complement(24102..24836)
                     /locus_tag="ROI21_002936"
     CDS             complement(24102..24836)
                     /locus_tag="ROI21_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HDX5111836.1"
                     /translation="MKYISLFLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSFASVQYENISVVLSKRRDIIEQAPTYEINEPVFAYDKTMPVSILLA
                     LMSIATCWLLWKYRAILTNLIRLKNK"
     gene            complement(24833..25165)
                     /gene="sctI"
                     /locus_tag="ROI21_002937"
     CDS             complement(24833..25165)
                     /gene="sctI"
                     /locus_tag="ROI21_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDX5111837.1"
                     /translation="MSVSNMPPIDRAEQAAAREIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            complement(25185..25424)
                     /locus_tag="ROI21_002938"
     CDS             complement(25185..25424)
                     /locus_tag="ROI21_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HDX5111838.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            complement(25438..26619)
                     /locus_tag="ROI21_002939"
     CDS             complement(25438..26619)
                     /locus_tag="ROI21_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HDX5111839.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRVLVIVGND
                     VSLGRSDSFSELPENTIVIPYGELTGSFEVIITTDPDLVVTIRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKVKNEVWQYSIPGIVENNITSTRQHFFSSTLFKYVTLFFLFAIIFI
                     AFYIVNASNDPQLRHIDKILVNKNRNYEILYGRDHVIYINTNSLDEAVWVKQALEKNQ
                     PGKPVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDASNPRLTVSKQRNAITQQLID
                     NLIKGLLQTMPYASNISIAVLDDNVLESQAIETLSAIGISYEKYKTANNVYFNIIGTL
                     SDSELNKINNYVDGYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSKWTFQ
                     E"
     gene            26878..27378
                     /locus_tag="ROI21_002940"
     CDS             26878..27378
                     /locus_tag="ROI21_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX5111840.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNTLLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCIRES"
     gene            27408..27674
                     /locus_tag="ROI21_002941"
     CDS             27408..27674
                     /locus_tag="ROI21_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HDX5111841.1"
                     /translation="MIITVALISLLSACTRENSLKDTQNKISGESQAIVDARQFFSEH
                     PEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
     gene            complement(27744..28868)
                     /locus_tag="ROI21_002942"
     CDS             complement(27744..28868)
                     /locus_tag="ROI21_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDX5111842.1"
                     /translation="MECFTANKTEKPTQKKLQDASKKGQILKSRDLTVCVIMLVGTLY
                     LGYVFDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWF
                     QSKMQLATEAIKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIIFFALAIKLFWSNHK
                     SLLFKTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQE
                     VKREYKEQEGSPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHLS
                     PIPLISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEILR
                     LLLWLEDVENAGQPVPDELLPSEDKFKEGEDTKSENKDNN"
     gene            complement(28865..29071)
                     /locus_tag="ROI21_002943"
     CDS             complement(28865..29071)
                     /locus_tag="ROI21_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HDX5111843.1"
                     /translation="MIAVMLGGEAVLGLLARYASQLNAFAISLTVKSALAFLILIIYF
                     GPILAERVMPLSFFPEQLQLYIDK"
BASE COUNT         8752 a         6470 c         5741 g         8120 t
ORIGIN      
        1 tattgccagc gcaaacagcg caccgaagcc aatgccaatg ggcacgaccg ggatgcctac
       61 cattaccgcc agcgagtaaa tgcccagcat caacaacatg gcgctgtttt cgccaaggtt
      121 ttgtactgca attgcattcc ccgccccgac gcttttttta ccccgttcct gtagcaacgc
      181 attgagcgga acgacaaaaa agccccccag cacgccaatc agcatcaaca aggcataggc
      241 tggcagcagc tcgtgttgca gggagaaaat cagtaccacc acgccaatca aaatcccggc
      301 tggcatacag cgtgacacgg tttccagcgt aactaacttc gctgccgcac cagcgccaac
      361 cacgatacca atcgctacca tcgcgttgag ataggtcggc gtagcgttat cggtaatgcc
      421 cagcgccacc ggtacccaca gcaccaacag gaaacgcagc gtgacacctg ctccccagaa
      481 taaactggtg cccaccagcg aaaaacgcgt ttcaccattg cgccatagcg aggtgcaggc
      541 attcaggaaa ctgcgggtca tgttgatgag attccaggac tgccccggac gcgccgccgc
      601 cagtttggga atgtagatat tggcaacgac cgcaccaccg taggccagtg cgcatgcggc
      661 cagggcgacc aggacatgcc agtcagccag tacaccaccg gctacggaac cgagcaaaat
      721 cgccgctatg gtagaagctt ccattaaacc gttagctttc actaacttac tacccgtggt
      781 taattcgccg agaataccgt atttcgccgg tgaataggct gcagcaccaa cacccaccag
      841 cgtatagccg agaaacggat tgataccaaa gcagatactg gctgcgccca gcagcttcag
      901 gccgttggca aacatcatca cccggccttt ggcgaagcta tccgccacct gcccgacaaa
      961 cggcgcaaaa agaatgtaag cacctacaaa caccatttgc aggatgggct ggctccactc
     1021 cggatagaac tgcgctttca gtagcgctag agtggcaaac agtagtgcat tatcgccaaa
     1081 cgcagagaga aactgcgcca cgataaccgc tttcatcccc ttcgaccaca acgaagtgtt
     1141 agtgtgcact gactcactca tcgtgttgtt ccgcttcgtc tacccagctt ttcaacgtga
     1201 caaagtcagg tttgccgctg ccaagtaatg gcatctgttt cagatagcga atatcgcgcg
     1261 gtacagcaag ctccggcacg ccgtgctcgc gggcatactg ttgcaactta tcgcgcgtca
     1321 gttcgttatc tgtggtgaaa agcaccagcg cctcgccttt gctggcatcg ctcttaatcg
     1381 cagtggcatg gactttatct ggcgaaacac caagtgccag ttgttccacc atttccagcg
     1441 ataccatttc gcctgcaatt ttggcaaagc gttttgcgcg gccctgaatc tgcacaaagc
     1501 cctgctcgtc gaaacgcaca atatcgccag tgtcatacca gccgcgctcc atttcgccgc
     1561 gaacattctc ggcggtgggc acttccagta cacctggctt ctccacccgc agatagccgt
     1621 tcattatgtt cggccctttc agttgcaggc gtccgccctc ttcgatacca ggaaccgaca
     1681 acaggcgcgc atccattcct ggtagaatac gccctaccgt accgggtttc gccgccatcg
     1741 gtacgttgat ggaaacgaca ggcgcgcact cggtcacacc gtagccttca aggatccgca
     1801 ggccaaattt atcctgccaa agctgtttgg tactttcttg tagtttttct gcgcctgcca
     1861 ccacatagcg tagacggtag aagtcatacg ggttggcgaa acgcgcgtag tgaccgagga
     1921 aagtggaggt accgaacaac acggtgcaac tgcggtcata caccaactcc ggcacaatac
     1981 ggtaatgcag cgggcttgga taaaggaaca cttctgcacc tgtgagcagt ggcgtaaaca
     2041 ggcctaccgt cagcccaaag gagtgaaaca gcggtaacgc cgacataaag cgatcgttgg
     2101 tggtgaagtc ggcaatcgtt ttaatctgct cgacgttcgc cagaatgctt ttatggctat
     2161 ggacgacgcc tttcggatgg ccttcagaac cggaggtaaa aaggatcagc gcctcttctt
     2221 ccggctgctg ttttacctgt gccagacgcg gcatcagcaa atgagcgaag atccacactt
     2281 tgtcggcagt ggtgacatct gcttttaaat cttccagata gacccagcgc acctgagtaa
     2341 gttgctccgg cagatgccag agtttgcctt tatcgagaaa ctggcgggaa gtgaagatgg
     2401 ttttgatttc agccgccgta atagcactgg tcagcccttt taccccggcg gtgtagttca
     2461 tcattgcggg aatgcggcga cgggcgatgg ccccaaaaat cactgccgca ctgatgcctg
     2521 cattgggcag cattaagccg atgtgttcgc cttcaacact gtatttttca aggatgcgtc
     2581 caacaaacag cgttttcgta agcaatttgc gataggagtc tggggtaaag ttgacgtctt
     2641 cgacacattt cttcccggct ccgaagcggt acattgcact cagtaaagat tcgtacagcg
     2701 tttcacgcgg gcgcaccgcc atacgcgctt ccatcattat ttgatgcagc atttcgccag
     2761 cgattttgcg acggtcacgg gcacgcggcg catccggcat ctctacctgc gttggtggca
     2821 aaatatgcag agtaatttgc gggaacaagc gacgtttaac caggcctttc aggcggctga
     2881 agtgcgtaag ttccgcccct tcaatacgca caggaataac cgttgcacca gacttcgccg
     2941 cgacaaaacc cgcaccatcg tagattttca tcagcgagcc tgtggtggtg attcgtcctt
     3001 cagggaaaat caccactggt cggccctgtt ccaccagacg taccagatgt ttaatcgcca
     3061 taggttgcgt cgggtcgaga ggaacaaagt cgataaatga tttcagccaa cgcatatacc
     3121 actgttggct tattgaggtg taaacggcaa ataccggacg tacgggtaaa aacaatgcca
     3181 gcagtatgcc atcaataaaa gagacgtgat taggcgtgat tagaacgcgc tcgcccttca
     3241 gtgcctgggt gtcacccgta acgcgaacgc gatacaaaac acggcacaaa tttcgaaaaa
     3301 agctaaaaag cattccaact ccctttgctc tgattcagta aaagcgaatg gagggagatt
     3361 acacgagata aagaacgcga gcgacagtaa attaggtgcg aaaaaaaacc tgcgcatccg
     3421 cgcaggttgg tgcaagagac agggtacgaa gagcgtaccg aataatctca ccaatcaata
     3481 cctctgggat cttgattgtg gtctgcacga cgactcttcg ccagcgagaa aacgcaaagg
     3541 aatgaaggga aatgcaacga ggtgtgtaaa ttgtcggtta ctgttacaga ttgatgaccg
     3601 gcaaaaaaaa acctgcgcat ctgcgcaggc tggtgtaatt catgtgctca acccgaagtt
     3661 gacttcacct atcaatacct ctgggatcac cactttagca acctgaagcc aaacgccacc
     3721 agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt
     3781 gtcttactta tttgcgtttg gctcacattc ccacgatgaa aacacgccac cccttgaacc
     3841 aacgggcgtt ttccgtaaca ctgaaagaat gtaagcgttt acccactaag gtattttcat
     3901 ggcgaccata aaggatgtag cccgactggc aggcgtttca gtcgccaccg tttcccgcgt
     3961 cattaataat tcacccaaag ccagcgaagc ttcacggctt gctgtgcata gtgcaatgga
     4021 gtctcttagc tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac
     4081 ggtcggtctg gtcgttggtg atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt
     4141 cgaacaggtg gcttatcaca ctggtaattt tttattgatt ggcaacggtt accacaacga
     4201 acaaaaagag cgtcaggcta ttgagcaact gatccgccat cgctgtgctg cgttggtcgt
     4261 ccatgccaaa atgataccgg atgccgattt agcctcatta atgaaacaaa tgcccggtat
     4321 ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc tggacgatcg
     4381 ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa ggtcataccc gcattggtta
     4441 tctgtgttct aaccactcta tttctgacgc cgaagatcgt ctgcaagggt attacgatgc
     4501 ccttgctgaa agtggtattg cggccaatga ccggctggtg acatttggcg aaccagacga
     4561 aagcggcggc gaacaggcaa tgaccgagct tttgggacga ggcagaaatt tcactgcggt
     4621 agcctgttat aacgattcaa tggcggcggg tgcgatgggc gttctcaatg ataatggtat
     4681 tgatgtaccg ggtgagattt cgttaattgg ctttgatgat gtgctggtgt cacgctatgt
     4741 gcgtccgcgc ctgaccaccg tgcgttaccc aatcgtgacg atggcgacgc aggctgccga
     4801 actggctttg gcactggcgg ataatcgccc tctcccggaa atcactaatg tctttagtcc
     4861 gacgctggta cgtcgccatt cagtgtcaac tccgtcgctg gaggcaagtc atcatgcaac
     4921 cagcgactaa ccgcagttaa agcaattcca gtgccagtaa ttcttcgatg gtctggcgac
     4981 ggcgaattaa ccgcgcctga ccattatcaa acagaacttc tggtaacagc ggacggctat
     5041 tgtagttgga tgacattgac gcgccatatg cccccgtatc atgcagtacc agataatccc
     5101 ctgccttcac ttccggcaag gcgcgggttt caacatttcc cccttcctgc tgggtaaaga
     5161 catcgcccga ttcacataac ggcccggcga cgacggtttc caccgttggt gcgtgttcca
     5221 gtgaacgacc atcagctgcc agggcactga tatggtggta actaccgtac attgccggac
     5281 gcatcagatc gttgaacccg gcatcaacca gcacaaagtg gcggctaccc atttgtttga
     5341 cgctccgcac ctgagtaatt aacacgccag actgcgctac caggaagcga cccggttcaa
     5401 tttccagttt cacagggtgg cccaaatggc gggcgatttg ctcacgcgcg gcattccaca
     5461 gaccataata atgttcggta tcaaccgcct cttcaccctg ttgataagga atagaaagcc
     5521 caccgcccgc agaaatagcc tgtaaatcct gaccgaattc gaggacctga cgcaccatag
     5581 caccacatac ctgttccaga tgggcataat caacgccaga accaatgtgc atgtgaatgc
     5641 cgaccagctg tagatgatga cgttgtatca cgtccagtgc ggcgggcaga tcggtgtacc
     5701 agataccgtg cttgctgttt tcgccaccag tattggtttt ttggctatgc ccgtgaccaa
     5761 accccggatt aacacgcagc catacccgat gccctggcga aacctgaccc agttggtcga
     5821 gcatatcaac agaacccgca ttcaccggaa tttgcaattc actgacgcgt tcaagcgtcg
     5881 cctgatcgat aacatctgcc gtaaaaacaa tatcatcggg gtacgtttgc ggattgtaac
     5941 ccgccgccaa cgcacgctct atttcgccta acgagacgga atccactttc acgccctgct
     6001 cacgcattaa gcgcaaaata tgaatattgg aacaggcttt ctgtgcgaag cgcaccacat
     6061 caaactgttt cagcgctgca atctgccgac gaataatttg cgcatcgtag acccacaccg
     6121 ggcagccaaa ttctgcgggc aaacgcagca gattttcggc ggtgagatcg gtatcagtgc
     6181 tgaacagtga atgtggcata acaaactcca gataagtgct tttttatgat tacgccacat
     6241 cataaaaaga ataaaaaata tcgatttatg ttgagtctat gcaaaaatga tatggattac
     6301 cggattgcga gagagcgcta atggccgccg ttaacttacg tcatattgaa atttttcatg
     6361 cggtaatgac cgccggaagc ctgactgagg cggcacacct gctacacacc tcacagccaa
     6421 ctgtcagccg cgaactggcg cgctttgaga aggtgatcgg gctgaaattg tttgagcgcg
     6481 tacgtggacg attgcatcct accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat
     6541 cctggtacgg actggatcgc attgtcagcg ccgcagaaag tctgcgcgag tttcgccagg
     6601 gagaactgtc tattgcctgc ctgccggtct tttcgcaatc ttttttaccg cagctcctgc
     6661 aaccctttct ggcacgttat cccgatgtca gcttaaatat cgtgccccag gaatcaccgc
     6721 tacttgaaga gtggctctcg gcccagcgtc atgatttagg actcactgag acactccata
     6781 cgcctgcggg aacagaacgt accgaattac tctctttaga tgaagtgtgt gtgttacctc
     6841 cgggtcatcc gctggcggca aaagagatat taacgccaga agattttcac agtgagaact
     6901 acatcagcct ttcccgtact gacagctatc gccagttgct ggatcaattg tttactgaac
     6961 atcaggttaa acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg
     7021 tacgggcggg ggtaggtgtt tcggtggtta acccgctcac cgcactggat tatgcggcaa
     7081 gcggtttagt ggtgcggcgg ttcagtattg cggttccgtt caccgtcagc ctgatccgcc
     7141 ccctgcaccg cccgtcatca gcgctggtgc aggcgtttag tgagcattta caagcgggat
     7201 taccgaaact ggtcacttct cttgacgcta ttttgtcgtc agctacgaca gcataaaagc
     7261 gacagcatcc tcggcatgga tcgccgcggt atcaaacaca ggcagaacac tgcgctcttc
     7321 tggcaccagt aaaccaattt ctgtgcagcc aaaaataacg ccctgtgcgc cctgttctgc
     7381 aaggcgagca atcacttgcg cataataagt gcgtgacgct tcggtaaatt gccccagaca
     7441 cagttcttca aaaataatct gattaatttt cgcccgttca tccgcttcag gaatgagaca
     7501 gttgatggaa aattgttccg tcagccgccc gcgataaaaa tcctgttcca tggtgtaacg
     7561 cgtacccagc agcgccacac gagtcattcc agcccggata attgcacgtc cagtggcatc
     7621 cgctatgtgt aagaaaggca gagagcaacg agactcaatg gcatccgcca ctttgtgcat
     7681 cgtattggta cataacacaa taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc
     7741 agccagaata tccccggttt tgtcccactc accgcgacgc tggcactctt ctatttcatg
     7801 aaaatcgacg ctatgtagca gcacttgcgc agagtgaagc ccaccaagcc gctgtttaat
     7861 accttcattt atcaaacgat agtaaggaat ggtggattcc cagctcattc ctcccagcaa
     7921 accaattgtt ttcattcctc tctcctgatg tgtgttaccg caacaaggct gaataaggaa
     7981 cgagacaaac gcctcaacgg ccaggtgccc aatctctatt aacgaaaaaa gggctggatg
     8041 tacagcacat ccggcccgtg aaatcagacg ccgatatttc tcaacttctc gcctgccatc
     8101 agtttgcgtt cgatatgttc cagcgtgaca tttttggttt ccggaatgag ccagaaagta
     8161 atgcccacaa acgcaatgtt cagcccagtg tagagccaga acgtaccggc agcgccaatg
     8221 ctatcaagca gtgtcaggaa ggtcgcgccg ataatcatat tcgacaccca gtttgtcgtc
     8281 gttgagcagg tgatgccaaa atcgcggcat ttcagcggct gaatttcaga gcacaagatc
     8341 tacaccactg gcgcggcgct catcgcataa ccggcaatac acatcatcgt catgccaaca
     8401 gagagccagg acaagccact ggaagccgta ccgttatcaa actgcatcag gcagtaaccc
     8461 agcaccagag tgcctaacgc catcacgctg aaaccaattt tcagagccgg tttacgccct
     8521 gctttatcta ccgtaaagac cgcaatgaag gtggcgaaca taaaggtcag ccccacgacc
     8581 agagtcgcaa tcatctgctg ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc
     8641 gcgtagtaca tgatgatgtt cataccggta aactgctgca tcgcctgcaa caacataccg
     8701 agaaacacag cacgacggac gttacggttg atcttaaaca gtgcccaacc gccctgtttt
     8761 aacttcaggc tttcacgaat ttcgttgagt tcttctcgcg ctttttccga cgtatcacgc
     8821 agcatacgca acacttcttc cgcctcaata tgacgcccct tttccgccag ccagcgcggg
     8881 ctatttggca ggaagaccac cagaataatc agcaggactg ccggtaaggc aagaaccccc
     8941 aacattgcgc gccagttacc gctataactg aacgctgtat cggataaaaa cgccagcacg
     9001 atgccgagag tgaccatcaa ctggtacatg ctgatcatct taccgcgaac gttttcactt
     9061 gccatttcag aaagatacag aggagcggtg taagacgcga tcccgacagc aatgcccagc
     9121 accacacgag ctgcgattaa catctctacg ctggtcgcaa aagcggaccc tatcgagccg
     9181 agcacaaaca ggatggcccc cgccatcagg ctgtatttac gccccaggcg gaacgacagc
     9241 caaccattaa acagcgcacc aattgctgcg ccgagcatca tgctactgac cacccattcc
     9301 tgcaaacgac tggtcagtac aaagtgatcg gtaatgaacg gcaacgctcc ggcgattacg
     9361 ccgatatcaa gaccaaataa caatcctgcg accgcagcag ctaccgaaac aaacatattc
     9421 atacgccgcg tatcccgcaa agaacgtggc gttaaagtag attccgtatt gatagtaacc
     9481 attttttcct gccagcagag agtaagacat agtgaaaaaa tacgtgaaca attcacacag
     9541 gtgtcaggtc ggaaatagca taaatatgga ttaaattgct acgacatgtc gttatgtgat
     9601 ggatattcca attttcaaat taagttgaat aattgagatt attattaacc acctaatttt
     9661 aaagcagata aaactcataa agttcattaa ttgataattg atatggatta tttcataacc
     9721 atgatatgga ttatgatgat ctacaggtat aaaaaaccct gccatgcggc agggtcataa
     9781 aagtaagaga aatgaattaa cgagccagcc aaccgccatc cacggcaatg gtataaccat
     9841 tcacataatc tgaagcgctg gaggcaagga acactaccgg ccccatcagg tcactcggca
     9901 gtccccaacg gccagctgga atgcggtcga gaatttccgc gctacgttgt tcatctgccc
     9961 gtagttgttg agtattgttg gttgccatgt aacccggagc tatcgcatta acattaatgt
    10021 tgtgtttagc ccattcattc gccatcaatc gcgtcacgcc catcacgccg ctttttgatg
    10081 cggtataaga aggcacacgg atcccgccct ggaaggagag cattgacgcg atattgataa
    10141 tcttgccgcc attgccttgc gcgataaagt gtttcgccgc tgcctgagac atgaagaata
    10201 cgctcttgat attcaggttc atgacatcgt cccagtcttt ttcgctgaac tcgagagcat
    10261 cttcgcgccg aatcaatccg gcgttattca ccaggatatc aatatgacca aactcagcta
    10321 ccgcgcgatc cagcagtgct ggaatgccat caatctttcg cagatcggcg gtcaggctta
    10381 aaaaacgacg ccccagcgcc gtgacctgct tgatggtttc agtcggttca acgatgttaa
    10441 tgccaacaat gtcacagccc gcttgcgcca gccccaacgc catcccctga cccagtccag
    10501 tatcacaacc agtgacgacc gcaactttac cttcgagaga aaatgcactt aaaatcataa
    10561 caatacctta ttcgtttatg cccacaacta gcgcaaatct ttaacggcca catggtccat
    10621 atcatcaaag acctggtttt caccgaccat gccccagata aaggtataag ctttggttcc
    10681 gacaccggaa tggatcgacc agctcgggga gatcaccgcc tgctcgttat gcatcacaat
    10741 atgacgcgtt tcttgcggct gccccatcat gtggaaaacg caggcgtcat catccatatt
    10801 gaaatagaaa taaacttcca tccggcgctc gtgggtgtga cacggcatgg tgttccacaa
    10861 gttacccgga gccagctccg tcagccccat actcaattgg caggtttcca gtacatccgg
    10921 gacaaaatat ttgttaatcg tgcgacggtt actggtgagg ttatcgccta acgtgactgg
    10981 agatacttcg tccggtgtga cttttttggt gggatacgtc gtatgcgcag gtgcgcaatt
    11041 gtaataaaac ttcgccggag tggcggtatc aatactggca aagacaactt cttttgcacc
    11101 tttaccaaca tacagggcgt cgcggtgacc gatttcatag cattgaccat cgacagtaat
    11161 cgtaccggca ccgccaatat tgataacacc taactcgcga cgttccagga aatagcttac
    11221 gccgagttgt ttaccaactt ccccgccaac ggaaaccgtt ttagttaccg gcataatgcc
    11281 gccaacaata atacggtcaa tgtggctgta aaccatggtg tactcatcgg cgataaatac
    11341 cttttcaacc aaaaattcat tgcgcagccc ctgggtatcc agcgtttttg catgcgcact
    11401 gtggatgctc tgtcttacgt ccacatcaac ctccgataat cagtgaagcg tgaaaataga
    11461 aaacgatagt gaaacactgt ttcgttttct ctataagggg atgatatgcc cacgggatag
    11521 ggttttcaat taaaaataaa acattgtttc atttttaagt taaggactga aaaaagtgca
    11581 gtgtcgatca cgaatatgtc atgagaatgg gaaaatcagg gagccctgaa aatgtatgtc
    11641 gactcgccag ccaccattat tcaacagatt aatgacggct gatatttatc ggtaaggagg
    11701 aaatgcagag ctattcgtca cgttcaatgg tcaatgccac gccctggccc ccgccgatac
    11761 aaagcgtagc cagtcctttg cgggcattac gtttcaccat ttcatgaacc agagacacga
    11821 ggattcggca accggaagcg cctattgggt gaccgagtgc gatcgcgcca ccattgacat
    11881 tgacccgacg ctcatcccat tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg
    11941 cttcattagc ctcgataaga tcgacatcag ccaactgcca gccaacacgc tccaggcaac
    12001 ggcgggtcgc atacaccggc gcaattccca tcaatgccgg atctacacca acgctggcaa
    12061 atgcgcggat gcgggccagc acgggtaaat tcaacgctcg tgctttggct tcgctcatca
    12121 tcattactgc agctgcgcca tcgtttatgg atgatgcatt acccgctgtc acagagccga
    12181 gactatcaaa tgaaggattt aaacgggcta agccttctgc actggcgtca gtgcgtggct
    12241 gttcatcggt atctacaacc aacgtctgac cgttactttg ggtcattacc gggacgatct
    12301 catctttaaa tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt
    12361 aagcatcctg caactgacgg ctgatgccat attcgcgagc cagattttcg gcggtgacac
    12421 caatatgata atcattgaag gcatcccaca acccatcgtg cacaagacta tcaaccaact
    12481 ggctattacc aagctgtgca ccggtgcggc tatcagtcag aacatgtggt gcgcgactca
    12541 tgttttcctg accaccggcg atgacaatat cagcctcgcc acactgtatc gcctgagtag
    12601 ccagatgcag tgctttaagc ccggaaccgc aaacgtcatt aatcgtgatt gcagaaacgc
    12661 tattaggaag accaccttta atagccgatt gccttgccgg attctgccct gcccctgcag
    12721 tcaacacctg accaagaatc acttcatcca ccgcatatgc agaaacaccg gtacgttcta
    12781 ttaacgcttt cacgaccagg ctgccaagtt ccacagcgga atgacccgct aacgcaccac
    12841 gaaagcagcc gataggtgtc cgtaacgccc cgacaatcac aacgtctttc atcacaacct
    12901 cgcaccaatt aacagtgcaa tagtaaatga ttgttaattg cagttatctt aattgtttaa
    12961 aaaaagtgat ttttatcaca aaggaaatat gcctgagtag cagtcagaga cataactggc
    13021 acgtaaggtt tgcaaccact aacccaccaa tagagaggta gaaagggcgt taatctccca
    13081 tacttaacct ggtttatggt aaattgccct ccattttgtt taatttgtag atgatacgtt
    13141 cagataatgt ctaatatttg gtcaaaagaa gaaactctgt ggagtttcgc gctctacggc
    13201 acagccgttg gtgcaggcac gctcttcctt cctattcagt taggttcggc aggggctgtg
    13261 gtcctgttta ttactgctct ggtcgcctgg cctttaacat attggccaca taaagcctta
    13321 tgccagttca tcctctcatc aaaaacatca gcaggtgaag ggataacggg cgcagtaaca
    13381 cactactatg gcaagaagat tggtaatctg attaccacgc tgtacttcat cgcctttttt
    13441 gtcgtcgtgt tgatatatgc cgtggcaatt accaactcac ttacggaaca gctggcaaag
    13501 catatggtta ttgatcttcg tatccgtatg ttggtgagtc tgggtgttgt attaatcctg
    13561 aatctcattt ttctgatggg acgccatgcc actattcggg taatgggatt tttggtattc
    13621 ccattgattg cctatttctt atttctttct atttacctgg tcggtagttg gcaacctgat
    13681 ctattaacta cccaggtaga gttcaatcag aatacccttc accagatatg gatatcgatt
    13741 cccgtgatgg ttttcgcctt tagccatacg cccattattt ctacgtttgc catagacaga
    13801 cgtgaaaaat atggcgaaca cgctatggat aaatgcaaaa aaattatgaa agtcgcttat
    13861 ctcatcatct gcataagtgt actgttcttt gtctttagct gcctgctttc tattccacct
    13921 tcgtatattg aagcggctaa agaagaaggg gttaccattt tatcggcact ttctatgctg
    13981 ccgaacgccc cagcatggtt gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg
    14041 aaatcattcc tgggtacgta ctttggcgtt attgaaggtg ccacagaggt cgtcaaaaca
    14101 acattacagc aggttggtgt aaagaaaagt cgtgcattta accgcgcact atcaattatg
    14161 ttggtatcgc tgattacctt cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt
    14221 tacgcgatca gcggcccgct cattgccatg atactgttca tcatgcctac gctgtcaaca
    14281 tatctcatcc cggcgcttaa accctggcgt tccgtcggaa atctgattac gctgatcgtg
    14341 ggtatcctgt gcgtatcggt aatgttcttt agctaatatc cattgtgaat ggttcaaaat
    14401 tgctacaatt ttgaaccatt ccagttttca taatgttcat ttgttataat tccgctctat
    14461 cacttctctc gtcatatcct agtattatca gttagttaaa cacacaaaga gatctgaaca
    14521 tcttaaataa gactatttaa gatgcataac ttagattcgc aagatatctt ctggtcattc
    14581 aaaaacaatt tgcgatatag atattctcat accatgagat agttatctga accaccatca
    14641 accaggtaag attaaatcag taaaacataa tcatatttaa taacgagaaa tgcattttta
    14701 aatgctttac tcagaattat agcaaattca gattaatcgc aatttttccc agcaaattta
    14761 caaaatcatg gtgtattgac atcatcaata accaatgaaa ttatgcaatt atatacggat
    14821 agggaggttc ttaacatggg gctttgtagt cgttataaaa gtcttacatg caatagttgc
    14881 tcaatgcatt gccaaataat gccagaagag tcaccgcgtt tgcagtattg cgcaaactcg
    14941 tgtttttgta tgtggcccga agaaagttca tattttaatc gtggtgtggt ggaaggtatt
    15001 ttaacaaaaa accacaatgc gagattaagc ggatatattt ttgtcgattt ttcagtaagt
    15061 tttttacgtc tattccttga aaaagactgg attgactatc ttgcaagtac tgacatgggg
    15121 attgttttag tcagcgaccg caatatgcaa tcgttagcta actactggcg aaaacacaac
    15181 tctgcaatat ctgctgtcat atataatgat gatggtcttg atgttgccaa tgaaaagatc
    15241 agacaactat ttattggtcg ttatttatca tttaccagag ggaacacatt aactcagatg
    15301 gaattcacca taatgggata tatggtttct ggttataatc catatcagat tgctgaagtt
    15361 ctggatatgg atatccgtag catctacgcg tacaagcaac gaatcgaaaa gagaatgggt
    15421 ggtaaaataa acgagttatt tattcgttca cattcggtcc aacattgata ctcaataatc
    15481 tgccagaatc atactctgct tatacgggca tctattgaaa tgcccgtatt ttacaaataa
    15541 gcagtaaccc caagagtaaa caacatggta tttaaaacca tcacacgaaa aattcagaag
    15601 cattacgaaa tatgaattta caaacaatag tggcataaat gttaatcatg ttaatttacg
    15661 taaagtttta cgttgcgaca ttaaagcctc atttcaatca ttatgataaa tataaaatta
    15721 atatatattt atgccgtaaa tatcaatata cttagcaaac tatgtgattt ccatttcgat
    15781 tgatttagtg tctattgacg tatgtactgg attattaacg ataatatcga gttctggcct
    15841 gagcaccgaa aattaatatc ggtacataac gccgatctta acgtcgttct gacaacgcca
    15901 gccagtcgtt gtttatcact tctacttgaa gcttttcctg atgtggttgc acaacaagat
    15961 tttttcacca gagtctggga agaagaaggt atgcgtgtgc caactaacac gttatatcag
    16021 aacatatcca ttatcagacg cggatttcgc gctgttggtg ataccaccca ctcgcttatt
    16081 gcaaccgtgc cgagaagagg attcaagatt cataatgaca ttaacataca aaatcatgca
    16141 ataaactctt caacagacgc acatacacac aatgccccac ctgccgtaaa aggtaatgcg
    16201 gggtacaaag agagcattgg aagcgcaaac aatttcaata acaaaatcct caaacatata
    16261 aaaagttatc taattatgtt gagcatattt gctataggcg tatattctgc acattggcta
    16321 tggaataata atcaaccgaa acctttcttc aaggattaca agactgttgc ggaaattaat
    16381 ggctgccatt ttaatgtgac agaagataca attgatgggt tgaaagagtt cgataaatat
    16441 aagacacgga tactggattc ggggattaac tgtaaaaaat atccgtggtt atacttccct
    16501 cttgcaaaat cctcaccagg gatgattgtt atggcatgca ataaaaacta taaccaacat
    16561 gaagtggcag gctgtttaac cctgtcttac cgtgaggtta atcgtgattg attataaaaa
    16621 aaacttatta tttattttag tttttatcag tgggtttatt ctcttcattg tttactctta
    16681 tactgcagaa aaaatgatat ataatgaaac atgcactgca aattgggtaa tttttaatga
    16741 caaggggcga gcaaatctta ccatcgactt tatgtataac caaaaaaata aaacaggaac
    16801 tgttgcgttg agcggaacct ggcaacaagg taacagagaa agtaagtcaa tacggcgaaa
    16861 tattgaatac acatggattg aaaactatga cacagcccat ttaacatcaa agaaagttaa
    16921 taaatttgag atcatggatc aagtcgatga tgatagactt gcagaactta ttcctgattt
    16981 ttatgtcttt ccagaaaaaa gtgtaagcta taatatacta aagcaaggta agcatgcttt
    17041 tattttgagt attggtaaca gagcaataat gcattgtgca aggtaaactt aaatagataa
    17101 taaaaagatg gaacctcgtt aatcataaaa caataaaatt aaatatgctt gtagtatatg
    17161 ccagtataaa gcatcaaagt tttgatcaac atctttcatt ttagatatca ccttgcaatg
    17221 caaaatatca tgtatcaaca acatctggtg caaatccatg agtcgaactc aacattcatc
    17281 ttagctctat tattattcct tcgtacagtc gatgtaaaca atacagaaag aggattatta
    17341 agaaccagtt tacttagaga atcaatagag gaaaggacta acgtttcttt aaaagaattg
    17401 atttcatcat ctgttaaact aaactcatca ttgacagatc gtgagatata actgttttta
    17461 actttactct ttacgttgac tttactgaca gaattaacat tcacatatct tgaatttagt
    17521 acccatgatg ttgtttgcgt gagaaccttc tcagcattaa ggaatttttt taacggaatc
    17581 cttggtttct ttttcgagtc cgaatttaca atatcatgca tatagaccat ttcatgaatc
    17641 tgcgaaaact caatgtccat tatatacctc acattttgac cgtgactcga tgttactgtt
    17701 caataatcac cttccatcaa tactaaattt aataccccta atgtgccgat aacaaatata
    17761 gtcattctac gtaacgtctc cataaggtga taaccttgat caaatgaatt tcgaaaataa
    17821 taaaggaaat cttttgagat gtttgtcacg aataaagcta tttatgttac caatatcatt
    17881 tcgagtgtgc acactatcta tctgtttttt caggatataa aaaacaacat gtaagttatg
    17941 taaattgccg atgccactaa gtaacattat ggtaaaaaaa gtaaaaatga gcgatttttg
    18001 atagaataaa gcatgagaaa aggtgatatt tgacattatc agaagctgcg aattcggatt
    18061 ttgctctaat caaatgagag aaatgtaaaa tgaagccacg aaatattaat aatagcctac
    18121 cactgcaacc attagttcct gatcaggaga acaaaaataa gaaaaatgaa gagaaatacg
    18181 tgaatccagt taaaattacg atgaggtctg gtttaaatta tattgaacaa gaatctcttg
    18241 gaggaaaata tctaacacat gatttgtcaa taaagatagc ggatatttct gaagaaataa
    18301 ttcagcaagc aatattatcg gctatgagca tatataaatt tccgataaca gatgatttaa
    18361 tgagtatggc tacaaatgaa ctcataaaac tggccaaaat agagaataat ttagacctga
    18421 ataaattctc tactatatgt accgacgttc tatcccccct cgtcaccaga cataataaag
    18481 aaaaaaaacc agacgactta ctacccttcg caaaaatccc ttttttaatt ttcatcgaaa
    18541 aaatggaaat tgataatatg aatccaggcc atgcacatta aggcatttat gaaatgtcgt
    18601 aattaagata actaataagg taaatattag caaaacaact aatgacatta ttggtttata
    18661 gtggtcagta tttttactaa taatgaacca atatacccat aagcaatgca agcttaaaac
    18721 tcataattca aaaaagataa atgcaaaaaa ttaatgagtc aatcaataat aatttagcat
    18781 cacgaaacac atcacagagg aatattatga gcacagatac acttgaaata ttcaataaca
    18841 gtgatgaatg ggcaagtcaa ctaaaacacg cattatcaca aggagaaaat ctggcattac
    18901 tacatggatt gactcctgat atccttgata gaatatatgc atatgcattc gactaccatg
    18961 aaaaaggtaa tataacggac gcagaaattt attataaatt tctgtgcatt tatgcgttcg
    19021 aaaatcatga atatctaaaa ggttttgcat cagtatgcca gtcaaaaaag aaatatcaac
    19081 aagcatatga cctttacaaa ctaagttaca attactcacc gtatgatgac tattcagtta
    19141 tttatcgtat gggtcaatgt caaattgggg ctaaaaatat cgataacgca atgcaatgtt
    19201 tctatcatat tattaacaat tgtgaggatg aaagtgttaa gagcaaagcg caggcatata
    19261 ttgaactctt aactgataat tcagaagata atggctaagc attacaatct tttggttaaa
    19321 attgctggtt atagtgcagt taactactat gaccagcttc tcttatgttt tcttaaatca
    19381 acatcttaaa acagttaaga acacactata aattattatt tttcaacgcg ttagagaaca
    19441 atctatttta tcaaaattga ataaggctat ttcagattac atcactgaat tcctaagata
    19501 aattagtgaa cacaaccata gatatgtcga atgtaaactc ctgtagcatt aatgacacat
    19561 aaatatgaat agccacaatt tctattgctt atgcaggatg caagaaacca attttttcat
    19621 agaggttaac taatggactt agaaaataaa ttctcatatc attttctcga gggattaacg
    19681 ctcacggaag atggaattct tactcaagga aatgagcaga tttatattcc accgaaagag
    19741 ttaggagtat tgatcgtatt acttgaatcc gctggtcatg ttgtcctgaa agatatgatc
    19801 atcgaatcag tatggaaaaa tattattgta agtgacgagt ccctgacaag atgtatctat
    19861 tctttgcgct gcatttttga aaaaattggc tatgctcgtt gcatagaaac aatctaccgg
    19921 aaagggtatc gtttcagtgg acaggttttc aaaactaaaa taaatgaaga taacacttca
    19981 gactattcca tagctatatt tcctttcacc acttcattga atactctgga tccattaata
    20041 cttaatcagg aattagtgca aaacatttcg aataaaaaaa tcgatggcct ttatacctat
    20101 ccgatggctg cgacaaattt ttgtaatgat cacatatcgc aaaattcatt tttgagaaga
    20161 ttcaagccag attatttcgt tacaggaaga ataaaccaga ataacgcggt gaaaacttta
    20221 tacattgaac tgatcgacgc taaaaatctt ttcctcatcg ccagtaatca tcttcctgtt
    20281 gatgaactac ataacacatc acaatttatt atagataata tccttcaaac ggtacatatt
    20341 ccagagcgtt ctgtaagatt agctaaaccg gaccaaggtt acaagaatca ttatttatca
    20401 gatgaactgt tggctggtaa gaaagaactt tacgagttta ctcctgaaag catttacagg
    20461 gctatggcta tatttgatgg attacaaaat aaaagtgata tacagacgct aaaaatagaa
    20521 tgttattgtc tgctagcgga atgccatatg tctttggcac ttcatggaaa gtatgaactt
    20581 gaacttgctg ctcaaaaagc attagagctt ttacattatg tatcagacat aaccaatgtc
    20641 gatggaaaaa ttttagccat tatgggactg ataactggtc tgtctggaca agcaaaagta
    20701 tctcatatct tatttgaaca ggctaagata cacacaactg atatagcctc tctctactac
    20761 tatagagcac ttgtcaactt tcataatgaa agaattgaag aggcaaggat ttgtatagac
    20821 aaatcactac aactcgaacc cagaagacga aaagcagttg tgataaaaga gtgtgtagat
    20881 atgtatgtgc ctaacccgct caaaagcaac atgaaactct actataaaga aacttcgagt
    20941 gaaagccatc gagttataat tgataacatt ttgaaactaa agcaactgac gagaatttgt
    21001 atgcgataaa tatttattgt cgcaatttta ttgtttagat gtatgcaact ggttatgtaa
    21061 acaacagaga tgaaaacatc taagataatt accttattgc aatatatatc aaatactcta
    21121 gattaaaagt attgtgtatg ggctattggc ctggacaaat aaaaaaggga actttatgac
    21181 taatccaatc aatatgaata atttttctca aagtttaaat atagcaaccg caacgggtga
    21241 tgaagtcgtt tcattagata aacacatcac caccagtgct actgatacag atcaaataca
    21301 agcatttata gtttcaacat ggatggcatc ttttcaaaat gatatgtatt cgaaagataa
    21361 tcctatcttt ccttactatg aactggaatg gtagataatt gaccacttaa atgattaacg
    21421 agatactgaa tatattagta tctcgttcct ttccttcaat cctacacata aaaacatatg
    21481 cgcttaccca aagttaaaat cttagccatc ttcatgtttt ctatattaat tacaaaaatg
    21541 tctttcgcca gtagcgcttg ctttaatgaa gcaggatcaa ttttcagaat cgaaccaaat
    21601 ttgatcaaag ctatcgcact tgttgaaagt aatttaaaaa atgatagcat cggcaagaac
    21661 agggataaga aaaacaatat aaagagtttt gattatggtt taatgcagat aaaccagatg
    21721 cacattccca tgttaaaaaa acgtggaata ataaaagatg aacaagattt gttagataac
    21781 ccctgtctaa acattaaaat agggaccgag attttatata atcatttttc acgttgtgga
    21841 gtaacgtggc aatgtctggg gacttataac gcgggttttg caatggaaaa ccaaaaaaag
    21901 aggctgcaat atgcaaaaaa aatatatgtt gtatatacca gacttaatga aatagacaag
    21961 cgtaaggctt tagcaaaatg agcctaaagc ctcttttttt ttatatagtg caaacgccca
    22021 tacgtaaagc tgttttgatc aactcgatac cactgtgaac atctaatttc ttcatgatat
    22081 tgagtctatg tgtttcgact gttttcctgc ttaactgcaa gaaattagcg atttctttgt
    22141 ttgtttttcc atcagctatg agttgtagta tttcagactc acgatttgta agttgattat
    22201 cagaggaata tcttgaagat attttttcac aatccatgtg tacactatca aaataagtgt
    22261 aaccattact aatcgaatca atagcccgat ttagtttcgc cgggtgactg gttttccata
    22321 cgcatccttt tgcaccggct tcaagcaatt ttattgaatc aatatacggc atttttgcta
    22381 caaatgtaat aatatgagca tctattttat gcgatttaac ccatttaatg agttcggacc
    22441 ccatcattcc acctgcaaat tctcctaaaa taaatatttg ggctgcagat ttgttacact
    22501 ccgctatagc tttcttcaga tctttatagc ccccaacaac ctcatataaa tcgggataat
    22561 gtccgagaaa tccaattatc ccatcaatca taaacggctg ctgatctgaa actacaattt
    22621 taatctttcc catatattca tcctgagtgt aaatgctata acgcattcat ttataatcat
    22681 cccttccatt attattataa gcaaaatcca aaaaatacat tgatgaaata ataatgaaat
    22741 ataattaata ataaaatttt tgcgtaagca gaataacacc cgtgttgaaa aatcattagg
    22801 cgtttaaaat attagaatta gattatattc aagtcattaa gccggagaca gtgatctata
    22861 agtcctctgg ggtcgtcaaa tcagaattat tcatagccaa agaatctatt tcacacattc
    22921 tttcaactaa ataatgtctg gcatgttcac agatttcatt aaccttgtca ggggaaaaac
    22981 agtgattgct aattaaatat tgctcacaat tatccacaaa ttcctgcgga ctaaactcag
    23041 caataaattg atctgtaaaa aaaacaaaac gtttgtcatc atgatatgaa atatggacat
    23101 tccatattga cttgtcgaca aaatatgact ctacttcaat agacttatct ttgaaactgg
    23161 taggtatata gagatttaaa gggcctgata ttgacggtaa ttttgttacc caactttcaa
    23221 aaattttgat aaaaacttca acttcctctg aagattttaa caatatgctc ttcagatcgt
    23281 ttatcatatt tattctgttt ttttctaatt ctgagctcag caaacttatc aagggtataa
    23341 aattatccat cacatcaata atgcctttcg catagccatc ataataacct tgaataactt
    23401 tttcttctct cttttcctca atactctgaa tttttgcggt ggtttcaaga aggatctgtt
    23461 cttttagaga aactatttct ttcaatccat cagaaattat tcttttaatc acaacaccat
    23521 ttgcaggaga gtttatcaag ctcatctcta ttttttttcg cataaaatgt aattctctcc
    23581 aaaagagtag acgcaagtgg taacggcaat tgtatgtggt caacaaaatc aggaaataat
    23641 aaattaaatc gttgctgcat tgccaacggt tgttgcctta catagggcaa caacgttgaa
    23701 aaaccacatg tgatgatatt ctgatagtta acgataccag gtttatatcg tgccttttca
    23761 tttatttcca ccggtatagc tgataaataa ttccttagca catcaggaac cttgtaaaaa
    23821 aatccgcgtt ctgcaaacct ttctcgataa aagtgacaac cactgagtaa acagatcaat
    23881 ggaaataatt tccaattgtt tatgtagtaa ataagtgaac tattcagact gaaatgctca
    23941 actgaaagat tgtactgtag gagtatcatt tcattggtaa tagatctaag taccggattg
    24001 ctaatacgtg cattattcaa acttactcgt tgaggatgaa tataactaat tggatcatat
    24061 ataatctttc ttagggcaag attcataacg ttctcatatg tctacttatt ttttaacctg
    24121 attaaattag ttagaatagc cctgtacttc cataacagcc agcaagtcgc aatggacatt
    24181 aatgcaagta agatagaaac cggcatagtc ttatcataag caaaaacagg ttcgttaatt
    24241 tcatatgttg gtgcttgttc aataatatct cttcgttttg acaatacgac tgaaatattc
    24301 tcatattgta cgcttgcaaa gctattaaca ataaatctct taatgtcatt tatttttatt
    24361 tctggattga tatctttttc atatacagca agtacagaaa tatgaattgg taaagcagtt
    24421 ttgccactat cacctgtatc aacatcgtaa ctaacatgta ctcttgaaga aacaatgcca
    24481 tccataattt tgagagattg ctctaaacgc tgttcaatag cagaatacaa tcttgctttt
    24541 tccgctcgtg gagaggaaac gagcgcatct gcagggaaca tctgcgatat ttgaatatca
    24601 ggcttacccg ggagattgta gatttttagc caatccaccg cagaagcaaa atccgttggt
    24661 tctacataga ttgaaaatcc agttttgcct tgatccttct tctcagcatt aatattatgt
    24721 ctttgtaaaa cagcaaggac atcattagct tgctgttgat caagatggtt cagtaattcc
    24781 tgctgcttgc aaccgcacaa cagcaggata aacaagaata atgatatata tttcatgagc
    24841 gagtcaacgt ctcaactgca gcgacaattt ttcttgctaa tgttcccacc aatgaaacct
    24901 cgacattata attaagcaca tgatcctgca agactaataa tttttcagga gttaacccct
    24961 ttttatcaga ccttaatgct tgtaacgcat tttgctgcca attttcagtt tgtaccgcat
    25021 agttggcaaa agcggaaatc attttatcat ccgggttatc aagattgaga acccggtcat
    25081 ttaaatcaat aactttcgct tgctgtattt cacgagcagc tgcttgctca gctctatcaa
    25141 taggtggcat attgcttact gacataataa aatccttatc cagtttagcg gaagttttgt
    25201 atgattgccg aatcaagatc tttatacgct tttacaacgt tagattgcgc atttcgatat
    25261 aatgtatact cagctaatgc actctgatat tcggcaagga atttcgggtc ggaaggattg
    25321 gttgctaaat cctccaacgc cttttcaact tgttggttca gatcatcaac gccctgatca
    25381 aattgtttgc tgatatccat aatataacca ttccaatctg ccataagtcc tcctcaatta
    25441 ttcctgaaat gtccacttgc taccttgaga cttctcgaat cggttatctc cgtagctaaa
    25501 tgaactatta tttgtatcct gatttttcaa attcacatta aatcttacat attgtttacc
    25561 ccattgctta taatatccgt cgacatagtt attaatttta tttaattcac tgtcgcttaa
    25621 cgtaccaatg atattaaaat atacattgtt agctgtttta tatttttcat aagaaatacc
    25681 tattgccgac aatgtttcaa ttgcctgact ttccaataca ttatcatcta ataccgcaat
    25741 acttatattg ctggcatatg gcatagtttg cagtaatcct ttaataagat tatcgattag
    25801 ttgctgtgtg atagcattcc gttgcttact cactgtcagt cttggattac tggcatctaa
    25861 tagctgtagt ttaaaatatt gtagatctgg gaaattatca gcaagccatg aaaaaatccg
    25921 tatcgattca tcatcaggat ttatcacccg tactggcttt cctggttgat ttttttccag
    25981 tgcttgtttg acccagacag cttcatccaa actattggta ttgatataga tgacatgatc
    26041 cctaccatat aaaatttcat aatttctatt tttgtttaca agaattttat cgatatgtct
    26101 cagctgcgga tcattactgg cattaacaat ataaaaagct ataaaaatga tagcaaaaag
    26161 aaaaaatagc gttacatatt taaacagcgt actactaaag aaatgttgtc ttgttgaagt
    26221 aatgttattt tcaacaatac ctggtattga atactgccaa acttcatttt ttacttttac
    26281 agcaaatttg aggcctaaaa cttctatttg ctgattcaat gttaacgttc tatcttcagg
    26341 ttcttgtgct gttaattctc tgattgttac aaccagatca gggtcggtag tgattattac
    26401 ctcaaaactg cctgtaagct ctccataagg aatgactatc gtattctctg gtaactcaga
    26461 aaaagaatca cttcttccta acgaaacatc attaccaacg ataacgagta cacgcccatt
    26521 aagtatttca tactcgcatc cattcaatgg tccgctcaat aaacgaatgg cataagattc
    26581 caataagtct tgtgataaga atttatcatt attttccatg aataccaccc gctaattcca
    26641 taagctctag tttagagtag tgtaacaaga cggaaatttc atgaccaaat tatgatgaca
    26701 cacaataaaa agattataat tttcgagaac cattttaaat gcctacatac ataactttat
    26761 ttgcaattta aggtatacac cctattccct cattgatatg caaatataac aattaccatt
    26821 attttaaatt ttacacgtaa tatttaccct aaatgatttt agagtgcagg ttggcatatg
    26881 caagtctttt ccagtgatgt ctacttcact gtaggcacta atgcattatt agcatcccaa
    26941 aaggaatatt acagcgattt ggttgcgttg gttgatcttg gacattcgtt cgttgttatt
    27001 gatgagcacc aacatcgaaa tctcaaacca aatacagaac ctgtaaacac ccttttgagc
    27061 aacaatttca tcaggatcaa taagaatatt acattatctg atttaacgca tttcctggtt
    27121 tcgaatcttc atacacaaaa tgtgtattcc actcaagaac cacttaccca tgatgagata
    27181 gatatcctgc ggttatgtgt ttcttattcg ttaaaacaaa tagccattat caaaggaatt
    27241 gattataaaa cagtatcata tcacaaaata agagccctaa ataaacttaa cataaaaggt
    27301 actgttgagt tgtttatagc actctgtgaa tgggataaac attattttaa actccaatct
    27361 tgcataagag aaagttgata tgtcattagt aatgatataa tagtaaaatc ataataactg
    27421 tagcgctgat atcgttgctc tctgcatgca ccagagaaaa ttcattaaag gatactcaga
    27481 ataaaataag tggcgaatct caagcgatag tagatgcaag acagttcttt agcgaacacc
    27541 cggaatatat atcatcagca gaactagagc agaagctata tcgcgaattt gcgcaggtta
    27601 ctactgttcc agcttataaa gagatgagta tgtatcaatt gcttgtcatt tcgcaagatc
    27661 gtttaacaca ttaaataaga atctacacgc tttttgcgtg tagattctta taaatttccg
    27721 actatgatag atgtttaatt tttttaatta ttgtctttat tctcactttt tgtgtcttca
    27781 ccttctttga atttatcttc tgaggggagc agttcatctg gaacaggttg tccagcattc
    27841 tccacatctt caagccacag cagaagacgc aagatctcat ctatattttc gaagctaaca
    27901 taatcgtagc gacgatgggt agcataaatt tttcgtgcta attttttatc tgtaataatt
    27961 ggtattccga tttcctttgc atatttcctt acggctaatg ctacctcatt agtttctctc
    28021 acagaaatca atggtatagg tgaaagatgt ggcttaaagt atatccctat ggcaatgtgc
    28081 gtagggttgg caatcatcag acggctatta ctgacatcag atttcaattg ctctgacaga
    28141 atttcttgat ggcgttctct gcgtttagat ttaatttctg gacttccttc ttgttccttg
    28201 tattctcttt taacttcttg tttatccatt ttcatatctt tcataaataa aaaatattca
    28261 gcgataaaat caaaaattaa gacaattatc atactgccga ggcaatacag tatgagaagg
    28321 aatagcatct ctccccaatc tgataataaa gaaatgatat ctccatcaag agttttaaaa
    28381 agcagtgatt tatggttact ccaaaataat ttgatcgcca atgcaaaaaa aataatataa
    28441 agtattgctt taacaaattc ttttacggtt tttaagccaa atatacgttt taagccattt
    28501 actggattta atgaatcaaa cttaaactta atagcttcag ttgctaattg cattttactt
    28561 tgaaaccatg aaacaagtat tgttgtaaac atacaaacca gcaaaaaagg aatgatcgtt
    28621 ttcaaccaac ctatccccat agctttaaaa tagtcccaaa tatccggttt agcgttatga
    28681 tcaaggatat attcaagaat cgacataatg tgatgcacat caaatacata tccgagatat
    28741 aaagtaccca caagcattat tacacagact gttaagtctc tactttttag aatttgacct
    28801 tttttagaag catcctggag tttcttttgt gttggctttt cagttttatt tgccgtgaaa
    28861 cactctattt atcaatgtat aattgcaatt gttctgggaa aaaggataac ggcataactc
    28921 gttcagccag tattgggcca aaataaataa ttaatattaa aaatgccagc gcacttttga
    28981 cagtcagtga gatggcaaaa gcatttagtt gtgaagcata acgtgccagc aatcccagga
    29041 cagcttcacc gcccagcata accgcaatca cggggcttgc gta
//
LOCUS       DAPOZO010000062        28489 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPOZO010000062 DAPOZO010000000
VERSION     DAPOZO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.062"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            185..229
                     /locus_tag="ROI21_002944"
     CDS             185..229
                     /locus_tag="ROI21_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111844.1"
                     /translation="MFSKLAQSSIKAMF"
     gene            490..813
                     /gene="iraM"
                     /locus_tag="ROI21_002945"
     CDS             490..813
                     /gene="iraM"
                     /locus_tag="ROI21_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HDX5111845.1"
                     /translation="MKWIVIDTVIQPTCGISFSAIWGDMKMIIWYQPTIFLTPGSIFT
                     PVKSGIIFKDKEYPITIYNITPFNKDLWSLLKSSQECPPGESEITNKCLHNSCIIKTC
                     PYGLK"
     gene            complement(916..2166)
                     /gene="icd"
                     /locus_tag="ROI21_002946"
     CDS             complement(916..2166)
                     /gene="icd"
                     /locus_tag="ROI21_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDX5111846.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWMKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMDGAKLLKCSEFGDAIIKNM"
     gene            2749..3198
                     /locus_tag="ROI21_002947"
     CDS             2749..3198
                     /locus_tag="ROI21_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111847.1"
                     /translation="MDTTEERNGTYFYRGHPNLSKIELLNVIFLEEFCKELGIDSVSG
                     AAILSGQPWLGTRAKPQGATLGTSVVSKYARILMRDARMPFGISVHTPVGVRMQKSNK
                     LAAIIARWVPWIGWASLSMSLYRVSTRTQEKYNLIAKPQDRIKWTSF"
     gene            3183..3533
                     /locus_tag="ROI21_002948"
     CDS             3183..3533
                     /locus_tag="ROI21_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1493 family protein"
                     /protein_id="HDX5111848.1"
                     /translation="MDFLLMATTITDEVLALFRDEIPGYQDDHGKEIPLELDSDLFDY
                     PRDDLEDAIRKYKERFGINLDNLDWSLYLPWESAPLLQRWFKLKREEVETTRLPLTVR
                     MFAESAEAGKWLYQ"
     gene            3866..4504
                     /gene="rluE"
                     /locus_tag="ROI21_002949"
     CDS             3866..4504
                     /gene="rluE"
                     /locus_tag="ROI21_002949"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDX5111849.1"
                     /translation="MKYFSDSKSSFRKKQVSRLNSQHSTKRKPENQPTRVILFNKPYD
                     VLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGK
                     IYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGVELVDEPAWLWPRNPPIRERKSIPT
                     SWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            4514..4975
                     /gene="nudJ"
                     /locus_tag="ROI21_002950"
     CDS             4514..4975
                     /gene="nudJ"
                     /locus_tag="ROI21_002950"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDX5111850.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSRQRYPLEMIGDFNWPFTKGVI"
     gene            5029..6135
                     /gene="mnmA"
                     /locus_tag="ROI21_002951"
     CDS             5029..6135
                     /gene="mnmA"
                     /locus_tag="ROI21_002951"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDX5111851.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPA"
     gene            6171..6812
                     /gene="hflD"
                     /locus_tag="ROI21_002952"
     CDS             6171..6812
                     /gene="hflD"
                     /locus_tag="ROI21_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDX5111852.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            6816..8186
                     /gene="purB"
                     /locus_tag="ROI21_002953"
     CDS             6816..8186
                     /gene="purB"
                     /locus_tag="ROI21_002953"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDX5111853.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            8356..9027
                     /gene="phoP"
                     /locus_tag="ROI21_002954"
     CDS             8356..9027
                     /gene="phoP"
                     /locus_tag="ROI21_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDX5111854.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            9027..10487
                     /gene="phoQ"
                     /locus_tag="ROI21_002955"
     CDS             9027..10487
                     /gene="phoQ"
                     /locus_tag="ROI21_002955"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDX5111855.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            10563..11684
                     /gene="roxA"
                     /locus_tag="ROI21_002956"
                     /gene_synonym="ycfD"
     CDS             10563..11684
                     /gene="roxA"
                     /locus_tag="ROI21_002956"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDX5111856.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(11733..12959)
                     /gene="pepT"
                     /locus_tag="ROI21_002957"
     CDS             complement(11733..12959)
                     /gene="pepT"
                     /locus_tag="ROI21_002957"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDX5111857.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERAEMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            13209..14345
                     /gene="potA"
                     /locus_tag="ROI21_002958"
     CDS             13209..14345
                     /gene="potA"
                     /locus_tag="ROI21_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDX5111858.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            14329..15186
                     /gene="potB"
                     /locus_tag="ROI21_002959"
     CDS             14329..15186
                     /gene="potB"
                     /locus_tag="ROI21_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDX5111859.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            15183..15977
                     /gene="potC"
                     /locus_tag="ROI21_002960"
     CDS             15183..15977
                     /gene="potC"
                     /locus_tag="ROI21_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDX5111860.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            15974..17020
                     /gene="potD"
                     /locus_tag="ROI21_002961"
     CDS             15974..17020
                     /gene="potD"
                     /locus_tag="ROI21_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDX5111861.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            17078..17539
                     /locus_tag="ROI21_002962"
     CDS             17078..17539
                     /locus_tag="ROI21_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDX5111862.1"
                     /translation="MSQDSRVFFRIFLGIGLVLILISVVVFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPSSKKTRRKRKSRP"
     gene            17536..18321
                     /locus_tag="ROI21_002963"
     CDS             17536..18321
                     /locus_tag="ROI21_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDX5111863.1"
                     /translation="MKKIILLLSLLILLPLTATSNPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGIAPFIVVVPGVNEASFRQNGKAMLAHASSSLSDVKGSVLLLFTTRE
                     PRLIMITNGQVESGLDDKHLGLLVENHTQAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDPPGSTTILGLFAWAVAFIVFTAFFYYTTRLNYALKFAVAMTIA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(18458..19279)
                     /gene="cobB"
                     /locus_tag="ROI21_002964"
     CDS             complement(18458..19279)
                     /gene="cobB"
                     /locus_tag="ROI21_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDX5111864.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19295..20206)
                     /gene="nagK"
                     /locus_tag="ROI21_002965"
     CDS             complement(19295..20206)
                     /gene="nagK"
                     /locus_tag="ROI21_002965"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDX5111865.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADKRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20235..21479)
                     /gene="lolE"
                     /locus_tag="ROI21_002966"
     CDS             complement(20235..21479)
                     /gene="lolE"
                     /locus_tag="ROI21_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDX5111866.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21479..22180)
                     /gene="lolD"
                     /locus_tag="ROI21_002967"
     CDS             complement(21479..22180)
                     /gene="lolD"
                     /locus_tag="ROI21_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDX5111867.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     SALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22173..23372)
                     /gene="lolC"
                     /locus_tag="ROI21_002968"
     CDS             complement(22173..23372)
                     /gene="lolC"
                     /locus_tag="ROI21_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDX5111868.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23633..24706
                     /locus_tag="ROI21_002969"
     CDS             23633..24706
                     /locus_tag="ROI21_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDX5111869.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLSTFQHPLSEVLS
                     KCWIYYNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            24834..28280
                     /gene="mfd"
                     /locus_tag="ROI21_002970"
     CDS             24834..28280
                     /gene="mfd"
                     /locus_tag="ROI21_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDX5111870.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVGNIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVDPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
BASE COUNT         7103 a         7232 c         7093 g         7061 t
ORIGIN      
        1 aacgggcgtg ccatacgccc gtttcaatac ttaacgcaca tgtgttttgg tttagtcatc
       61 atccggttat atgtatttta gccaggaatg ggttaaacca ttcctatata actaaaaaat
      121 tgaaacctta ttctcatgtc atgcttatat tcgtcattat cgttatataa aaaggccaac
      181 cataatgttt agcaaattgg cacaaagtag tataaaggct atgttttaat tacaggatgt
      241 tcagtcattt gaatgtataa cattttagct aaacaaatct aaaacgaagt gaataattta
      301 atgttttcac taaatctcat tttgtttaat atctattgag atttcttgct ttaaattttg
      361 ttttatataa acaatggttt taatttttaa ttaatttgtt tttttgaggg ggtaatatac
      421 tcatatgcaa aatcaagaaa taaacatcct aatgaaccat attaaatacc gtgggataag
      481 acataacaaa tgaagtggat agtaattgac acggtaattc aacctacatg cggtatatct
      541 ttttcagcca tatggggtga tatgaaaatg atcatctggt atcaacctac tatatttctc
      601 actcctggaa gtatatttac accggttaag tctggtatta tctttaagga taaagaatac
      661 cctattacta tttataacat cacaccattc aacaaggatt tatggagttt actcaaaagc
      721 agtcaagagt gtcctccagg agaaagcgaa ataacaaata aatgtttaca taatagttgt
      781 attataaaaa catgcccata tggactcaag taatggtctt catataaata ttctcgacat
      841 gctattaata atatccacat aaataaaaca acgggcgtat aacacgcccg tttcaatatt
      901 taacacatgt agagattaca tgttcttgat gatcgcatca ccaaactctg aacatttcag
      961 cagtttagcg ccatccatca gacgctcgaa gtcataggtt acagtcttgg cgttgattgc
     1021 gccttccata cctttaacaa tcaggtctgc ggcttcagtc cagcccatat ggcgcagcat
     1081 catctcagcg gagagaataa tagagcctgg gtttactttg tcctgaccgg catatttcgg
     1141 cgcagtaccg tgggtggctt caaacagggc gcattcgtca ccgatgtttg cgcctggggc
     1201 gataccgata ccgccgacct gtgcagccag agcgtcagaa atgtagtcac cgttcaggtt
     1261 catacaggcg ataacatcat attcagccgg acgcagcagg atctgttgca ggaatgcgtc
     1321 agcaatcacg tctttaatga cgatctcttt gccggtattc gggtttttaa ctttcatcca
     1381 cgggccgccg tcgatcagtt caccgccaaa ctcttcacgc gccagctggt agccccagtc
     1441 tttaaacgcg ccttcggtga acttcatgat gttgcctttg tgcaccaggg tcacagagtc
     1501 acgatcgtta gcaattgcgt attcgatcgc tgcacgaacc agacgtttgg tgccttcttc
     1561 cgaacacggc ttaataccga taccgcaatg ttccgggaag cgaattttct tcacgcccat
     1621 ctcttcacgc aggaatttaa tcactttctc ggcgtcggca gagtcagctt tccattcgat
     1681 acccgcataa atgtcttccg agttttcacg gaagataacc atatcggtca gttcaggatg
     1741 tttaaccggg cttggggtgc cctgatagta acgtaccgga cgcaggcaga tgtagagatc
     1801 cagttcctgg cgcagggcaa cgttcagaga acgaataccg ccaccaaccg gagtggtcag
     1861 agggccttta atggcaacgc gatattcacg aatcagatca agggtttcag caggcagcca
     1921 gacatcctga ccataaacct gtgtggattt ttcaccggtg taaatttcca tccaggagat
     1981 tttacgctcg cctttatagg ctttctcgac tgcagcgtcg accactttca gcatggctgg
     2041 ggttacatct acaccgattc catcaccttc aatgtaaggg ataatcggat tttcaggaac
     2101 gttgagtttg ccgttttgca gggtgatctt cttgccttgt gccggaacaa ctactttact
     2161 ttccattcac ctctccttcg agcgctactg gtttgctcgt ctgccaccac gttgcatatg
     2221 cgtttgcgtc ctgcgatacg gatgcattag agcaattttt tgttaatgat ttgtaattgg
     2281 cttgtcaata ctaccctatt gtttgccgtc atgaaagatg cgcgttatta ggctataatg
     2341 cggcaattca taatctctga aaataccatg caaaaaactt cttttagacc aaagacagta
     2401 tttttcagac cttcaaataa ggcattaaat atcaataagt tacaccagtt tttaagcaat
     2461 aaaaaaaaac caccagagat aactaacagg atgctatttg gtcaaaattt gacattactc
     2521 gactcagcga ctatctcact ttgagcacta ttgggaaaat tatcaaaagt catggttgaa
     2581 gcgacaagca acgagtaaat tatggcatcc cccctttttg aggttcacat gaaacgcttt
     2641 gcactgatag ctgtgaaact cgttgccttt actggttctg catctgctgc aaccgaagaa
     2701 atcaacttca agtaacagaa acactatata acaatggagt gttgacaaat ggatactacc
     2761 gaagaacgca atggaacata tttctacaga ggacacccga acctgtccaa aatagaacta
     2821 ctcaacgtca tattccttga agaattctgt aaggaattgg ggattgattc ggtatcaggt
     2881 gcagcaatct tatctggtca accgtggctg ggtacaagag cgaagccgca aggggcaacc
     2941 ctgggaacaa gtgttgtctc aaagtatgcg cgtatactga tgagagatgc acgcatgcct
     3001 tttggtattt ctgttcatac accagtgggt gtaaggatgc agaaatctaa taagcttgct
     3061 gcaattatcg ctcgttgggt cccgtggatt ggttgggctt cactgtcgat gagtctttac
     3121 agggtttcga ctcgtaccca ggaaaaatac aatctgattg ccaaacctca agaccgtatt
     3181 aagtggactt ccttctaatg gcgacaacaa tcacagacga agttttagca ctgttccgtg
     3241 atgagattcc aggttatcag gatgaccacg ggaaggaaat tccgttagaa ctggactctg
     3301 atttgttcga ctatccacga gatgatttag aggacgctat acggaaatac aaagagaggt
     3361 ttgggattaa cctggataat ctggactggt ctttgtacct tccatgggag tctgcaccat
     3421 tgcttcaacg atggttcaag ctcaagcggg aagaggtaga aacaacacgt ttaccgttaa
     3481 ctgtacggat gtttgcagaa tcagcagaag cgggaaaatg gctgtatcag tagaataaaa
     3541 aagaaggctg cgatgcccct cttattactg ctgatgatgg tagtggtaaa gcatcatcct
     3601 gaacttgaac agtgaagtgc tgtcacccat cagtaataca gaaacactat ttttcatacc
     3661 agtatttatt caattcatca atgtaaaatg tgttcattgg gccatattta tcaccatgca
     3721 gccacacaac cgctgctcac taacccaggt attgatgaaa ccgcgctgaa gagcctgaac
     3781 cctgaacctc taacgagtac cttaaaagtc caatgcaatc cgacactgtg ctaccctctg
     3841 cgtatagctc tatttggaat ttaccatgaa atactttagc gattctaaat cttcttttag
     3901 aaaaaaacag gttagtcgac taaactcgca acactccact aagcgtaaac ctgaaaatca
     3961 gcccacgcgc gtgatcctgt tcaataaacc ctacgatgtt cttccgcagt tcaccgatga
     4021 agccggacgc aaaacattaa aagaattcat cccggttcag ggcgtttatg cagcaggtcg
     4081 tcttgaccgc gatagcgaag ggttactggt gctgaccaat aacggcgcgt tgcaggcgcg
     4141 tttaacccag ccgggtaaac gcaccggaaa aatctattat gtgcaggtgg aaggtattcc
     4201 cacacaagac gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg gccctaccct
     4261 gcccgcaggc gtagagctgg ttgacgaacc cgcgtggtta tggccgcgga atccaccaat
     4321 tcgtgaacgc aaaagtattc ccaccagctg gctgaagatc accttatatg aaggacgtaa
     4381 tcgccaggtg cgccgcatga ccgcccatgt tggtttcccc acgctgcgac tgattcgcta
     4441 tgcgatgggt gattactctt tggataatct tgccaacggc gaatggcgag aagcgacaga
     4501 ttaaggacgc aaaatgttta aaccgcacgt taccgttgct tgcgtggtgc acgcagaagg
     4561 caaattttta gtcgttgaag agacgattaa tggtaaagcg ttatggaacc aacctgccgg
     4621 gcatctggaa gccgatgaaa ccttagtgga agccgccgcc cgtgagctgt gggaagaaac
     4681 cggcatcagc gcgcaaccgc aacactttat tcgtatgcat cagtggattg cgccagataa
     4741 aacgccgttt ttgcgtttcc tgtttgctat tgagcttgag caaatatgcc cgacccagcc
     4801 tcatgacagc gatatcgact gctgccgttg ggttagcgcc gaagaaattt tacaggcgtc
     4861 aaatctgcgt tcgccgctgg tggcggaaag tattcgttgt tatcaaagca ggcaacgcta
     4921 tccgctggag atgattggcg attttaactg gccttttaca aagggtgtca tctaaaaggc
     4981 tgcgtgatag aatacgccgc cttgaagttc aatgtcgtga gtgatccaat gtctgaaacc
     5041 gcaaaaaaag taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc tgcctggctg
     5101 ttgcaacaac agggatatca ggtcgaaggt ctgtttatga agaactggga agaagacgac
     5161 ggtgaggaat attgcacagc ggcagcggat ctggctgatg cccaggctgt ctgcgacaag
     5221 ctcggcattg aactgcacac cgttaacttt gctgccgagt actgggacaa cgtcttcgaa
     5281 ctgttccttg ccgaatataa agccggtcgc acgccgaatc cggatattct gtgcaacaaa
     5341 gagatcaaat ttaaagcctt cctcgaattt gccgctgaag atttaggtgc cgattatatc
     5401 gctaccggtc attacgtacg tcgggcagat gtcgatggca agagccgcct gctgcgtggt
     5461 ctggacagca ataaagacca gagctacttc ctttatacgc ttagccatga gcagattgcg
     5521 caaagcctgt tcccggtcgg cgaactggaa aaaccgcagg tgcgtaagat tgctgaagat
     5581 cttggtctgg tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat tggcgaacgt
     5641 aaattccgcg agttcctggg ccgttatctc ccggcgcaac cgggcaaaat cattaccgtc
     5701 gatggcgatg aaattggtga gcaccagggg ctgatgtatc acaccctcgg tcagcgtaaa
     5761 ggtctgggta tcggtggcac caaagaaggc accgaagaac cgtggtatgt ggtcgataaa
     5821 gacgtcgaaa acaacattct gattgttgcg cagggccatg aacacccgcg tctgatgtct
     5881 gtcggtctga ttgcccagca gctgcactgg gtcgatcgcg aaccattcac cggcactatg
     5941 cgttgcacgg taaaaacccg ctatcgccag accgatatcc cctgcaccgt caaggcgctg
     6001 gacgatgatc gcattgaagt gattttcgat gagccggttg ccgccgtgac gccgggccag
     6061 tctgccgtct tctataacgg tgaagtgtgc ctcggtggcg gtattattga gcagcgtctg
     6121 ccgctgccgg cctgattatt ttctttactt aaaacaagga agcagtgaac gtggcaaaga
     6181 attactatga catcaccctc gccctggccg gtatttgtca gtcggcacgc ctggtgcaac
     6241 aactcgctca ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagcatta
     6301 tcgatatgaa ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg
     6361 tcgggctgga aaccttgctc ggcgtgctca atgccagcag tcgccagggc ttaaacgctg
     6421 agttaacccg ctacacactc agcctgatgg tgcttgagcg caaactctcc tcagcgaaag
     6481 gtgcgctcga cactctgggc aaccggatca acggcctgca acgtcagctc gaacacttcg
     6541 atttacagtc cgaaacgctg atgagcgcga tggctgctat ctatgttgat gtgattagcc
     6601 cgcttggccc acgcattcag gtaaccggtt cccctgctgt actgcaaagc ccacaagtgc
     6661 aggcgaaagt tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg
     6721 ttggcggcgg acgtctgcaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac
     6781 aaattcttgc tcatttaacc ccggagttgt gatctatgga attatcctca ctgaccgccg
     6841 tttcccctgt cgatggacgc tacggcgata aagtcagcgc gctgcgcggg attttcagcg
     6901 aatatggttt gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa aaactggccg
     6961 cgcacgcagc gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt taccttgatg
     7021 caattgtcgc cagtttcagc gaagaagatg cagcacgcat caaaaccatc gagcgtacca
     7081 ctaaccacga cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg gagatcccgg
     7141 aactgcacgc ggtttctgaa ttcatccact ttgcctgtac ttcggaagat atcaataacc
     7201 tctcccacgc gttgatgctg aaaaccgcgc gtgatgaagt gatcctgccg tactggcgtc
     7261 aactgattga tggcattaaa gatctcgccg ttcagtaccg cgatatcccg ctgctgtctc
     7321 gtacccacgg tcagccagcc acgccgtcaa ccatcggtaa agagatggca aacgttgcct
     7381 accgcatgga acgtcagtac cgccagctta accaggtgga gatcctcggc aaaatcaacg
     7441 gcgcggtcgg taactataac gcccacatcg ccgcttaccc ggaagttgac tggcatcagt
     7501 tcagcgaaga gttcgtcacc tcgctgggta ttcagtggaa cccgtacacc acccagatcg
     7561 aaccgcacga ctacattgcc gagctgtttg attgcgttgc gcgcttcaac accattctga
     7621 tcgactttga ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa cagaaaacca
     7681 ttgctggtga gattggttct tccaccatgc cgcataaagt taacccgatc gacttcgaaa
     7741 actccgaagg gaatctgggc ctttccaacg cggtattgca gcatctggca agcaaactgc
     7801 cggtttcccg ctggcagcgt gacctgaccg actccaccgt gctgcgtaac ctcggcgtgg
     7861 gtatcggtta tgcgctgatt gcatatcaat ccaccctgaa aggcgtgagc aaactggaag
     7921 taaaccgtga ccatctgctg gatgagctgg atcacaactg ggaagtgctg gcagaaccaa
     7981 tccagacagt tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg aaagagctga
     8041 ctcgcggtaa gcgcgttgac gccgaaggca tgaagcagtt tatcgacggt ctggcgttgc
     8101 cagaagaaga gaaagcccgc ctgaaagcaa tgacgccggc taactacatt ggtcgcgcca
     8161 tcaccatggt tgatgagctg aaataagcgt caaaacagtg ccggatggcg atgctgtccg
     8221 gcctgcttat taagattatc cactttttct tttttcactt tacctcccct ccccgctggt
     8281 ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa taattaagac
     8341 agggagaaat aaaaaatgcg cgtactggtt gttgaagaca atgcgttgtt acgtcaccat
     8401 cttaaagttc agattcagga tgctggtcat caggtcgatg acgcagaaga tgccaaagaa
     8461 gccgattatt atctcaatga acatttaccg gatattgcga ttgtcgatct cggtttgcct
     8521 gacgaggacg gtctgtcact gatacgccgc tggcgtagca atgatgtttc actgccgatt
     8581 ctggtattaa ccgcccgtga aagctggcag gacaaagtcg aagtattaag tgccggtgct
     8641 gatgattacg tgactaaacc gtttcatatt gaagaggtga tggcgcgaat gcaggcatta
     8701 atgcggcgta atagcggtct ggcttcacag gtcatttcgc tacccccgtt tcaggttgat
     8761 ctctcccgcc gtgaattatc tattaatgac gaagtgatca aactgaccgc gttcgaatac
     8821 accatcatgg aaacgttgat acgcaataat ggcaaagtgg tcagcaaaga ttcgttaatg
     8881 ctacaactct acccggatgc ggagctgcgg gaaagtcata ccatagatgt actgatggga
     8941 cgtctgcgca aaaaaataca ggcacaatat ccgcaagaag tgattaccac cgttcgcggc
     9001 cagggctatc tgttcgaatt gcgctgatga aaaaattact gcgtcttttt ttcccgctct
     9061 cgctgcgggt acgttttctg ttagcaacgg cagcagtagt attggtgctt tcgcttgcct
     9121 acggaatggt tgcgctgatc ggttatagcg tcagtttcga taaaaccacg tttcggctgt
     9181 tacgtggcga gagcaatctg ttctataccc ttgcgaagtg ggaaaacaat aagttgcatg
     9241 tcgagttacc cgaaaatatc gacaagcaaa gccccactat gacgctaatt tatgatgaga
     9301 acgggcagct tttatgggcg caacgtgacg tgccctggct gatgaagatg atccagcctg
     9361 actggctgaa atcgaatggt tttcatgaaa ttgaagcgga tgttaacgat accagcctct
     9421 tgctgagtgg agatcattcg atacagcaac agttgcagga agtgcgggaa gatgatgacg
     9481 acgcggagat gacccactcg gtggcggtaa acgtctaccc ggcaacatcg cggatgccaa
     9541 agttgaccat tgtggtggtg gataccattc cggtggagct aaaaagttcc tatatggtct
     9601 ggagctggtt tatctatgtg ctctcagcca atctgctgtt agtgatcccg ctgctgtggg
     9661 tcgccgcctg gtggagttta cgccccatcg aagccctggc aaaagaagtc cgcgaactgg
     9721 aagaacataa ccgcgaattg ctcaatccag ccacaacgcg agaactgacc agtctggtac
     9781 gaaacctgaa ccgattgtta aaaagtgaac gcgaacgtta cgacaaatat cgtacgacgc
     9841 tcaccgacct gacccatagt ctgaaaacgc cactggcggt gctgcaaagt acgctgcgtt
     9901 ctctgcgtag tgagaagatg agcgtcagtg atgctgaacc ggtaatgctg gagcaaatca
     9961 gccgcatttc acagcaaatt ggctactacc tgcatcgtgc cagtatgcgc ggcgggacat
    10021 tgctcagccg cgagctgcat ccggtcgccc cactgctgga caatctcacc tcggcgctga
    10081 acaaagtgta tcaacgcaaa ggggtcaata tctctctcga tatttcgcca gagatcagct
    10141 ttgtcggtga gcagaacgat tttgtcgagg tgatgggcaa cgtgctggat aatgcctgta
    10201 aatattgcct cgagtttgtc gaaatttctg caaggcaaac agacgagcat ctctatattg
    10261 tggtcgagga tgatggcccc ggtattccat taagcaagcg agaggtcatt ttcgaccgtg
    10321 gtcaacgggt tgatacttta cgccctgggc aaggtgtagg gctggcggta gcccgcgaaa
    10381 tcaccgagca atatgagggt aaaatcgtcg ccggagagag catgctgggc ggtgcgcgga
    10441 tggaggtgat ttttggtcgc cagcattctg cgccgaaaga tgaataaata tgtccatact
    10501 tcacgcatta cgttaagcat ccgttataat cggttgcaga taccagcctg tggatgctta
    10561 acatggaata ccaactcact cttaactggc ccgattttct tgaacgtcac tggcagaaac
    10621 gcccggtggt gttaaaacgc ggctttaata attttattga cccaatctct ccagacgagt
    10681 tggcgggtct ggcgatggaa agcgaagtcg acagtcgact ggtcagtcat caggatggca
    10741 aatggcaggt cagccacggt ccattcgaaa gctacgatca tctcggtgaa actaactggt
    10801 cattgttagt gcaagcagtg aaccactggc atgagccgac cgccgcgctg atgcgaccgt
    10861 tccgtgaact gccggactgg cgtattgatg acctgatgat ctctttttct gtacctggcg
    10921 gcggcgtcgg cccgcatctc gatcagtacg acgtgtttat cattcagggt accggacgtc
    10981 gtcgctggcg agtgggcgaa aagctgcaaa tgaaacagca ctgcccacat ccggatctgt
    11041 tacaggtcga tccgttcgaa gccatcatcg atgaagagct ggagcctggt gatattcttt
    11101 atattccgcc aggattcccg catgaaggct acgcgctgga aaatgcgatg aactattccg
    11161 tgggttttcg cgcgccaaat acgcgggaac tgattagcgg atttgccgat tatgtgctgc
    11221 aacgtgaact gggcggcaac tactacagcg atcctgatgt tccacctcgc gctcatcctg
    11281 cggatgttct gccgcaagag atggataaac tgcgtgagat gatgctcgaa ttgatcaacc
    11341 agccggaaca ctttaagcaa tggtttgggg agtttatatc ccagtcacgc catgaactgg
    11401 atatcgcgcc gccagagccg ccttatcagc cggatgaaat ctacgatgcg ctgaaacaag
    11461 gtgaagtgct ggtgcgtctg ggtggtctgc gcgtattgcg cattggcgac gacgtgtatg
    11521 ccaatggtga gaagatcgat tccccacacc gtccggcact ggatgcgctc gccagcaaca
    11581 ttgcgctgac cgcggagaat tttggtgatg cgctggaaga tccgtcattt ctcgcgatgc
    11641 ttgcggcact agtcaatagc gggtattggt tcttcgaagg gtaagtttga agttaagccg
    11701 gatgcggcat cacatgccgc atcccttttc gcttacttcc gttgcgccgt caactcggca
    11761 atacggacga tcacctgcac cgctttttcc ataccttcca gagtcacaaa ctcatgctta
    11821 ccatgatagt tgtaaccgcc agtgaacagg ttcgggcacg gtaatcccat aaacgacaac
    11881 tgcgcgccgt cggtaccgcc gcggatcggt ttcagttccg gttcaatatc gcagtcacgc
    11941 atcgcctgct gggcgatatc gagaatatgc ggatgctcaa ccactttctc gcgcatattg
    12001 tagtaactgt cttcaatcac caattcaatg tagcaatcag gatgtaaccc tttgcccact
    12061 tttttggcga tctccatcat tttacgttta cgcgcttcaa actgtttacg gtcgaaatca
    12121 cggatgatgt agtgcatttc ggcccgttca acggtgcctt tcatgctcgc caggtgatag
    12181 aaaccttcat agccttctgt catttccggg ctttcatccg ccggaacttc cgcatgaata
    12241 cgtgccgcca gcgacagcgc atttaccatc actcctttcg ccgtgcccgg gtgaacattg
    12301 ttaccgacaa ttttgatatt gaccgacgcg gcgttgaagt tttcaaactc cagttcgcct
    12361 acgccaccac catcaacggt gtaagcccag cgggcatcga aggcgtcaac atcaaaatgt
    12421 ttcgcccctt tgcccacttc ttcatccggg gtaaaggcga cgcgaatatc accatgcgga
    12481 atgtttttct gttgcaatac cgccagcgcg gtcatgattt ctgcaatacc tgctttgtca
    12541 tcggcaccta acaaggtttt accatcggtg gtaatcagcg tctgacccag tagctgatgc
    12601 agcaccggga acataaccgg tgataaaact tcatcgccga tacccagcgc aatatcgcca
    12661 ccgcgatagt tttcaacaat ttgcggattc acatttttgc cgctgcaatc cggtgaggta
    12721 tccacatgag aaataaagcc aatcgccggg atatcgccag ggacgttagc cggtaacgtc
    12781 gccatcaaag tgcccttctc acttaaggtc acattgataa gccccatctc ttcgagttgc
    12841 tctttcagca gatgcaataa cttccattgg ccttccgtgc tgggaacctg tctcacccct
    12901 gcttttgatt gggtatccag agacacgtag ttcaaaaatc gctcaagtag tttatccatg
    12961 tagtcaccct cactttttgt gacaacatta ttaagaagca agaaaagaca aatattgcgt
    13021 caggtcactt ttacccctgc aagcgggaat atttatcagc attaccttaa tgaatataaa
    13081 gctaagccag taattcacaa caaagattgg cgattcaagc ggtttgccgt agaattaccg
    13141 ccctcattaa gagtcaccaa ggtggttaac cacaaacccc gcatcggtaa gccatccgtt
    13201 gcgtttacat gggacagagt aaaaaattga ataaacaacc gagttcgctt tcaccgctgg
    13261 tgcaattggc gggaattcgc aaatgctttg atggtaaaga ggtcattccc cagctggatc
    13321 tgactatcaa caatggcgag ttcctcacgc tgcttggccc ttctggctgc ggtaaaacaa
    13381 ccgttcttcg cctgattgca ggtctggaaa ctgttgattc cggacgcatc atgctggata
    13441 acgaggacat cacccacgtt ccggcggaaa accgctatgt gaatactgtt ttccaaagct
    13501 acgcactttt cccccacatg accgtgttcg aaaatgtggc ctttgggttg cgcatgcaaa
    13561 aaacccccgc tgctgaaatt acgccccgcg tgatggaagc cctgcggatg gtgcagctgg
    13621 aaaccttcgc tcaacgcaaa ccgcatcagc tctctggtgg tcaacagcaa cgcgtcgcca
    13681 ttgcccgcgc ggtggttaac aagcctcgtc tgttgttgct ggatgagtcg ctctcagcgc
    13741 tggattacaa actgcgcaag caaatgcaga acgagctgaa agcgttacag cgtaagcttg
    13801 gcattacatt cgtcttcgtg actcacgacc aggaagaagc actcaccatg tcggacagga
    13861 ttgtggtgat gcgcgatggt cgcattgagc aagacggcac gccgcgtgaa atctatgaag
    13921 agccgaaaaa cctgtttgtt gctgggttta ttggcgaaat caatatgttt aacgccactg
    13981 tcatcgaacg tctcgacgag cagcgcgtac gcgccaacgt tgaaggccgc gaatgtaata
    14041 tctacgttaa cttcgccgtt gaaccggggc aaaaactgca tgttctgctg cgcccggaag
    14101 acttacgtgt tgaagagatt aacgacgaca accacgctga agggctgatt ggttacgttc
    14161 gcgagcgtaa ctacaaaggc atgacgctgg agtcggttgt tgaactggaa aatggcaaga
    14221 tggtgatggt cagcgaattc ttcaatgaag acgatcctga ctttgaccac tctctcgacc
    14281 aaaaaatggc cattaattgg gtagaaagct gggaggtcgt actggctgat gaagaacaca
    14341 agtaagttcc agaatgtagt gattgtcact attgtcggtt ggcttgtgtt gtttgtcttt
    14401 ctgcccaacc tgatgatcat tggcaccagc tttttgaccc gcgacgacgc cagtttcgtc
    14461 aaaatggtct ttacgctgga taactacacg cgtctgctcg atccgctcta ttttgaagtg
    14521 ctattgcact cgctgaatat ggcgctgatc gccaccctcg cctgcctggt gctgggctat
    14581 ccttttgcct ggtttctggc gaagctgcca cacaaggtgc gtccgctgct gctgtttctg
    14641 ctgattgttc cgttctggac caactcattg attcgtatct acgggctgaa aattttcctc
    14701 agcaccaaag gctatctcaa cgagtttttg ctctggctgg gcgttatcga tacaccaatt
    14761 cgcatcatgt tcacacccag tgcggtgatt atcggtctgg tttacattct gctgccgttt
    14821 atggtgatgc cgctgtactc gagtatcgaa aaactggata agccgttgct ggaggcggcg
    14881 cgcgatctcg gtgccagcaa gttgcagacc tttatccgca tcattatccc actgacgatg
    14941 ccggggatta ttgccggatg cttgttggtg atgctgccag cgatgggcct gttctatgtg
    15001 tccgacctga tgggcggtgc gaaaaacctg ctgatcggta acgtcatcaa agtacagttc
    15061 ctcaatattc gtgactggcc gtttggtgca gccaccagca ttacgctgac tatcgtaatg
    15121 ggcctgatgt tgctggttta ctggcgcgct tctcgcttgc tgaataagaa ggtggaactc
    15181 gaatgatcgg tcgactgctt cgcggcggtt ttatgaccgc tatctacgcg tacctgtata
    15241 ttccaatcat tattttgatt gtgaactcgt tcaacagctc gcgctttggc atcaactggc
    15301 agggttttac cactaaatgg tatagcctgc tgatgaacaa cgacagtctg ttgcaggcag
    15361 ctcagcattc attgacgatg gcggtgtttt cagcgacgtt tgctacgctt atcggttcac
    15421 tgactgcagt tgcgctgtac cgttatcgct ttcgcggtaa gccgttcgtt agcggaatgc
    15481 tgtttgtggt gatgatgtcg ccagatattg tgatggcgat ttctctgctg gtgctgttta
    15541 tgctgctggg tattcagctt ggcttctggt cgctgctgtt ctcgcatatc accttctgcc
    15601 tgccgtttgt ggtggtgacg gtgtattcgc gcctgaaagg ttttgacgtg cgaatgctgg
    15661 aagcggcgaa agatctcggt gccagcgaat ttaccattct gcgaaaaatc attctgccac
    15721 tggcaatgcc agcggtagcg gcgggctggg tgttaagctt taccctgtcg atggacgatg
    15781 tagtagtttc ttcgttcgtc accggaccaa gctatgaaat tctgccgtta aaaatttatt
    15841 cgatggtcaa agtcggcgta tcgccggaag ttaacgcgct ggcaaccata ttactggtgc
    15901 tgtcgctggt gatggtaatt gccagccagc ttattgctcg tgataaaacg aaaggtaaca
    15961 caggggacgt taaatgaaaa aatggtcacg ccacctgctc gcggcgggtg ctctggcact
    16021 gggcatgagc gccgctcacg ccgatgacaa caacacgctg tatttctaca actggaccga
    16081 gtacgtgccg ccaggactgc ttgaacagtt caccaaagaa accggtatta aggttatcta
    16141 ttcgacttac gagtcgaacg aaaccatgta cgcgaagctg aaaacgtaca aagacggtgc
    16201 ctatgacctg gtggttcctt caacctatta cgtcgataaa atgcgtaaag aagggatgat
    16261 ccagaagatc gacaagtcga agttaagcaa tttcagcaac ctcgatccag acatgctcaa
    16321 caagccgttt gacccgaata acgactattc cattccgtat atctggggtg caacggcgat
    16381 tggcgttaac ggtgatgcgg tggatccgaa atccgttacc agctgggccg atctgtggaa
    16441 acctgagtac aaaggcagcc tgctgctgac cgacgatgcc cgtgaagtgt tccagatggc
    16501 gctgcgtaag ctgggctact ccggtaacac caccgatccg aaagagattg aagctgcata
    16561 caacgagctg aaaaaactga tgccaaacgt cgcggcgttt aactccgata accccgctaa
    16621 cccgtatatg gaaggcgaag ttaacctcgg catgatctgg aacggttctg ctttcgttgc
    16681 acgccaggcg ggtacgccaa ttgatgtggt gtggccgaaa gaaggcggca ttttctggat
    16741 ggacagcctg gcgatcccgg caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa
    16801 cttcctgctg cgcccggatg tggcaaaaca ggttgcagaa actatcggtt atccaacgcc
    16861 aaaccttgcg gcgcgtaagc tgttaagtcc ggaagtggcg aacgataaaa cgctctaccc
    16921 ggatgctgaa accattaaaa atggtgaatg gcagaatgac gttggcgcag ccagcagcat
    16981 ttatgaagag tattatcaga agctgaaagc aggacgttaa ttcagcaagc gggcggtggt
    17041 attaccgccc gttttttatt tagcaaggat gtggctaatg agtcaggaca gtagagtttt
    17101 ctttcgtata ttcctcggta tcggtctggt actgatcctc atttctgttg ttgtctttta
    17161 taaccagttc acctatagca aagacgcgat tcatactgaa ggcgtcattg ttgataccgt
    17221 ctggcactcc agtcattctc atcgcacggg taaaaatggt tcgtggtatc ccgtcgttgc
    17281 ctttcgaccg acgcctgatt acacgctaat tttcaattcg agtatcggca gcgattttta
    17341 tgaagacagc gaaggcgata aagttaatgt ttattattct ccaggacatc ctgaaaaggc
    17401 tgagataaat aacccgtggg ttaatttttt taaatggggg tttatcggca ttatgggcgt
    17461 tatttttatc tccgtcggtt tattaatcag tatgccctcc tctaaaaaga cacgcaggaa
    17521 acggaagtcc cgcccatgaa aaaaatcata ttattgctca gtttactgat actcctgcct
    17581 ctgacggcaa ccagtaatcc attgattcca ataatgaaga ctctttttac agatgttaca
    17641 ggtactgtac cggatgctga agagatcgcg cataaagccg agctttttcg ccaacaaacc
    17701 gggattgcgc cgtttatcgt ggttgtacct ggcgttaatg aagcaagctt caggcaaaat
    17761 ggcaaagcga tgctggcgca tgcgtcatct tcattgagtg atgtaaaagg cagcgtcctg
    17821 ttacttttta ccacccgcga gccacggtta attatgataa ccaacggcca ggttgaaagc
    17881 ggtctggacg ataaacatct cggccttctg gtagaaaatc acactcaggc ttatttacat
    17941 gcggacctct ggtatcaggg aatcaataat gcattggctg ttttacaagc acagatatta
    18001 aaacaaccga cgccgccact gacatattat ccgcatcccg gacaacagca ggagaatgat
    18061 cctcccggca gtaccacgat attaggtctt tttgcctggg cagttgcatt catcgtcttt
    18121 actgcatttt tttactacac gacgcgcctt aattatgcat taaagtttgc agtggcgatg
    18181 actattgcca atatgggtta tcaggcttta tgtttgtaca tcgataatag ctttgcgatt
    18241 accagaatat cgccgctctg ggcaggacta attggtgtct gtacgtttat tgccgcgttg
    18301 ttattgacga gtaaacggta aaagaggcag taaagtgtgc gtctattatg ccggaggcgg
    18361 cgtaaacgcc ttatccggcc cacaaacccg caaattcaat taattgcgtc cccttgtagg
    18421 cctgataagc gtagcacatc aggcaattct tcccgcttta taagcccttc agcaactttt
    18481 caacaaactc tggcaccacc tggcttgccg ggccgtaata tttctcggca aattcattac
    18541 cgacctggct tggttcaaga ttcagttcca cggtgtgtgc gccatgcagt ttcgcttcgt
    18601 gaacaaaccc agccgccgga taaacatgcc cggatgtacc aatggcaatg aaaatatcgg
    18661 ccatcgacaa cgccatataa atttcatcca tgccgagtgg catttcgcca aaccacacta
    18721 cgtgcggtcg caagggtgcc gggaactggc agcaatggca tttatcttct ggggtaacgt
    18781 ctccggtcca gtcgagaacc tgaccacttt gtgaacaacg aactttcagc agttccccat
    18841 gcatatgaat aacattggtg ttaccggcac gctcatgaag gttgtcgata ttctgcgtca
    18901 ccaacaaaaa gcgatcgccg agggcgtctt gcagtttagc cagcgcaaga tgcgcggcgt
    18961 taggctgaat ttctggctgc tgcagctgtc ggcgacgggc gttataaaac gcttgcacca
    19021 gttcaggatc gcgatcgaaa ccttccggcg ttgccacatc ttcaacccga tgttcttccc
    19081 acaggccatc tgcggcgcga aaggtacgaa tacctgattc cgcagaaata cctgcccctg
    19141 tcagtacgag tactcttggt ttttccattg cttccggcac cactttatct ctgaaaaaaa
    19201 tacgctgacg caaacgctcg cgcagacggc gtttattttt acgaaaacga cttaaccgat
    19261 gaccccgacg cgacagcata gcaacctctg ttgtttaatc ggttagatgt aggaaggccg
    19321 caccacgcat tcctcccgca tcaccgtggc gcgcgcgttc aatgcgcgga acacgagcta
    19381 caggtaagag atgacgaggc agcctgtccg ccagttgcgt tgtgattgcc gggaaattcg
    19441 ataagccacc accaatgacg accaggtcag ggtcaacaat ggtcaggata tttcccagac
    19501 aaaccgctaa taaatccaga taacgctcaa cgtgcgccct tgcctgctca tcgccttgat
    19561 cataaagcgc aataatttcg ggagcctgca acggttgatg ataatagtgt tgatacagcc
    19621 acgcaaaacc gcgaccagac agataatttt caatgcagcc atgctgacca cagccgcagc
    19681 ggcgtaacgg gaaatccagc cccatcatgg ttaacgcatc aaccggcaga cgcatatggc
    19741 caaactcgcc ggtaatatag cttttaccgg taattggttt accgttgaaa atcagcccgc
    19801 cgccaacgcc ggtgccgaga atcaacccca tcaccagtgg atattgagta aattcatcat
    19861 cccaggcttc tgaaagggca aaacagttgg catcgttatc aaggcggaca tcgcgatcaa
    19921 gacgtgcgct caggtcggca cgcagcggtt taccgctggc agcggggaca ttggcggcat
    19981 acagcgtacc atcttctgtt tccggcattc ccggaatacc gatgccgaca gaacctttac
    20041 agccaaaacg cttatcggct tcagccacca gctcgcacac tgcatctaaa aatgcgtcat
    20101 agctgtcacg cggtgtcggc acccgctttt cccactgcaa ctgccgaccg ctatcaaaca
    20161 cgccaagcgc aatttttgtt ccaccaatat caaacccgta atacattgcc gctccttgtt
    20221 ttaatgtact gcctttactg gccgctaagg actcgcgcag ggtcaatatt actggcgcgt
    20281 cgcgccggat accaacttgc caaaagactc agcaacaatg ctgtgaccag tacgtagaag
    20341 acgtccagcc agtgcaattc cgatggcagg aagtcaataa aatagatatc gctggagagg
    20401 aactgatgac cgatcaactt ttcaatccac tcaataatcg gggtaagttg cagtgaaacc
    20461 actacgccga taatcacacc acacaggcta ccgaatagcc ctgccagcaa tccataccag
    20521 acaaagatgg cgcgaattaa accatctttc gcccccagcg ttcttaatac tgcgatatcg
    20581 ccactcttgt ctttcaccgc catcactaag gtggagacga tgttgaaaca ggccacgcca
    20641 atcaccagta ccatcgccag atacataatg gcgcggatca tctggatatc gcgatacata
    20701 tagccgtaag taccaatcca acttttaata taaacatagc tgttggtcac ttcacccgca
    20761 tcgcgtacca gcttattggc gttgaaaaca tccgtcattt taagggcaat acctgacacg
    20821 ctggaaccca tatcaagata ttgttgggca tctgccagcg ggatcatggc aaaactgtga
    20881 tcgagttgac cactcaactg caaaataccg gcaatgtgca aacgcacacg ttttggctgc
    20941 atcagtttat gctcgggatt cgagttgggg atcataatcg acacccaatc gccctgcttc
    21001 actttcagcg catccgccac gcctttgccg atgataattt gctgttcgcc cgctttaaaa
    21061 ttgcgccagg catccccctg aacaaacgag ggcaatgcgc tcagacgctg ttcctgttgc
    21121 gggttaacgc ccttcacctg gattgcgcgc agattcgcgc cgctctccac cagcccggtg
    21181 aaattgatat acggcgcagc ggcggcaatg cccggcactt tctgcacgtt atccagggct
    21241 tcctgccagt tagtccacgg ctgatctacc gcctcaattt caccatgcgg caccaccgcc
    21301 agaatgcggt tattcagttc gcgttcaaag ccgttcatcg cgcttaagcc gacgatcaat
    21361 accgccacgc cgagggcaat gccaatggta gaaatgacgg agatcagcga caccatgccg
    21421 ccgcgccgcc gtccgcgact aaaacgcagg ccaattaata acgataaagg catcgccatt
    21481 actccgcccc catcaggctc agttccgccg tcagacgtcc atcacgcatt tccagttgac
    21541 ggctcatacg tttcgccagt tgcaggtcgt gagtaaccac caggaaggcg gtgccctgca
    21601 agcgattcaa ctccccaagc aactggaaga tgctgtcggc gttacgcgca tcgaggttac
    21661 cggtaggctc atccgccagt accagacgcg gattattgac cagcgcacgg gcaatcgcta
    21721 cacgctggcg ttcgccgcca gaaagttcag atgggcggtg attcgcacga tgctccagcc
    21781 ccaccgcttt taacatctcc agcgcacggc tgttgatttc agcgggcttt ttcttgccaa
    21841 tcagcagtgg catagccacg ttttccaggg cagaaaaatc cggcagcagg tggtgaaact
    21901 gataaataaa gcccagcttc tggttgcgca gttctgcttt cgccgccgaa gacagtttgc
    21961 tcatcggctg accgttaaag atcacatcgc cggaggttgg cgtatccagc ccgcccagca
    22021 ggtgcagcaa ggtgctttta ccggaaccag agctaccgac gatcgccatc atttcacctt
    22081 cgccgacgct gaaactgaca ttgtgcagta catcggtttg cacactgcct tcctgatagc
    22141 gtttgcacag gttgtcgcat tgcaacagga tcttattcat aacgtaaagc ctcagcgggt
    22201 tgtgtggcgg cagcgcgcca tgaagggtaa agcgtagaca gcagcgcgat agccatcgcc
    22261 accagcgcaa taacaatgac ctgtaaaggt tcgatagcga ccggcagcgc cgcgccatca
    22321 agcaggacgc cgattatcgg catcagatta tttaactggc tggcaagcag tgcgccaagc
    22381 gccgctccga ggattgcacc gataatcccg gcgctggccc cttgcaccat aaagaccatc
    22441 atgatttgcc gcggagtcag cccctgagtc tgaagaatgg ccacttcgcc ctgcttctcc
    22501 atcaccatta accccagcga ggtaataatg ttaaacgccg caacggcgac aatcaggctc
    22561 agcagcaagc ccatcatatt tttttccatg cgtacggcct ggaacagctc gcctttacgg
    22621 tcacgccagt cctgccattt gctgccttca ggcagttttt gttgacttaa tgagtcgact
    22681 ttcagcggct catccagcca caatcgccag ccggtaatat tgcctgccgg ataacgcatc
    22741 agacgcgagg catcctcaat attcaccagc atttcatagc catcgacttc actgttggca
    22801 gcgaaagtgc caatcacatt gaacagacgc tggcttggaa tacgccccat cggcgtgaac
    22861 tggctggcag atggcaccat cacgcggatt tgatcaccgc gattgacgcc caactgtgag
    22921 gcaagctgtt caccgaggat gacattatat ttccccggct cgaggtcagt ttgtttcaca
    22981 ttgaccagat acggcgttag tggatctttt tgcgccggat cgatacccag catcaccccg
    23041 accgccacgc tgcgcgcgct ttgcagtacc acatcaccgg tagtaatagg tgcgacgcga
    23101 ttaacgccgt ccagtttgac tgccgtttcc gggagttgct gcgggttaag agagccatgc
    23161 tcagaagaga gaattgcctg tggcatcagg ccaaggatgt tgttttgcag ctcgcgctca
    23221 aaaccgttca tcactgacaa tactgtgacc agcgccatca ccccgagggt aatgccgatg
    23281 gtagaaagcc aggagacgaa acgaccgaag cgatccgctg cacgcccacg catgtaacgc
    23341 aggccaataa atagagcgac aggttggtac atgaaatccg tctggttgct gtagcaaaga
    23401 cacgagtata taagcgaaag cgggatgggt aaacgtctga actgtaagtg atcgtcaggg
    23461 ctaatctgaa aagtagcagc aaatcaatcg actaaagggc ctcattttct cattggcctt
    23521 gtggcctgat ctttatctga aaaataacca aatacagaca gatacgcatt acaccacgcg
    23581 actggcccct caggatcaac agcatgttaa ataaaaagag cgtgttgtcc tgatgaaaca
    23641 aaaagagcta tggattaacc agatcaaagg attatgtatt tgtctggtag tgatttatca
    23701 ctcggtgatt accttttatc cgcatctgag cacttttcag catccgttat cggaagtcct
    23761 gagcaaatgc tggatctatt acaatcttta ccttgcaccc tttcgtatgc cggttttttt
    23821 ctttatatcc ggctatttga ttcgccgcta tatcgacagc gtaccgtggg gaaattgtct
    23881 cgataaacgg atctggaaca tcttctgggt gctggcgctt tggggcgtgg tgcagtggct
    23941 ggcgttaagt gcgctgaacc agtggctggc acctgagcgc gatttaagca atgcctccaa
    24001 tgccgcgtat gccgattcca ccggtgagtt cctgcacggg atgatcaccg ccagcaccag
    24061 tttgtggtat ctgtatgcgt taattgttta tttcgtggta tgtaaaattt ttagccgtct
    24121 ggcgctgcca ctattcgcct tgtttgtact gatgagtgtg gcggttaatt tcgttcccac
    24181 gccgtggtgg ggcatgaaca gtgtgatccg caatttgcct tattacagcc ttggcgcatg
    24241 gtttggcgca acattaatga cctacgttaa agaggtgccg ttgcgccgcc atctgctgat
    24301 ggcttctttg ctgaccgttc tggcggtcgg tgcctggttg tttactatct cgctgctgtt
    24361 gtcgctggta tcgattgtgg tgatcatgaa gctgttttat caatacgagc aacgtttcgg
    24421 tatgcgctcc accagcctgc tgaatgtgat tggcaccaac accattgcta tctacaccac
    24481 ccatcgcatt ctggtagaga tattcagctt aactctgctt gcgccaatga acgcggcacg
    24541 ctggtcgccg caagtcgaac tgacactcct gatggtttac ccctttgtca gtttgttcat
    24601 ctgtactgtt gcgggcttgc tggtaagaaa actttcacag cgcgcattca gcgatctgtt
    24661 gttctccccg ccttcactgc ccgctgccgt cagttactcc cgctaagcca tcgccccagc
    24721 cggggcgatt caatttgcta aaccatgtcg attacggata atgaacagca ttgcttatca
    24781 gagatatgcc cccatatgtt gaggcatatc ctaacgagaa tctgacaacc gttatgcctg
    24841 aacaatatcg ttatacgctg cccgtcaaag cgggtgagca gcgtctgctg ggcgagttaa
    24901 ctggcgctgc ctgcgcgacg ctggtggcgg aaattgccga acgtcacgcc ggtccggtgg
    24961 tactcattgc accagatatg caaaatgccc tgcgtttgca tgatgaaatt agccagttta
    25021 ccgaccagat ggtgatgaat ctggcggact gggaaactct tccctacgac agtttttcgc
    25081 ctcatcagga cattatctcc tcgcgccttt ccacccttta ccagctaccg acgatgcagc
    25141 gtggcgtact gattgttccg gtgaatacgc ttatgcagcg cgtttgcccg cacagttttc
    25201 tccacggcca tgcgctggtg atgaaaaaag gtcagcgcct gtcacgagat gcattacgaa
    25261 cccaactgga cagcgccggt tatcgccatg ttgaccaggt gatggagcac ggcgaatacg
    25321 ccacgcgcgg cgcgttgctg gatctcttcc cgatggggag tgagctgcct tatcgtcttg
    25381 atttctttga tgatgaaatc gacagcctgc gggtgtttga cgtcgacagc cagcgtacgc
    25441 tggaggaagt agaagagatc aatctgcttc ccgcgcacga atttccgacc gataaagcgg
    25501 caattgaact gttccgcagc cagtggcgtg ataccttcga agtgaagcgc gatccggaac
    25561 atatttatca gcaagtgagt aaaggcacat tacctgccgg gatcgagtac tggcagccgc
    25621 tattcttcag cgaaccgctg ccgccactgt tcagttattt ccctgccaat acgctgttgg
    25681 tgaatactgg cgatctggaa aacagtgccg aacgtttcca ggctgacacg ctggcgcgtt
    25741 ttgaaaatcg cggcgtcgat ccgatgcgtc cactgttgcc accacaatcg ctctggctgc
    25801 gggtagacga gctcttttca gagctgaaaa actggccgcg tgtgcagcta aaaactgaac
    25861 atttaccgac aaaagcagcg aatgccaatt taggtttcca gaaactgcca gacctggccg
    25921 ttcaggcaca acaaaaagcg ccgctggatg cgctgcgtaa gttcctcgag tctttcgacg
    25981 gtccggtggt gttctcggta gaaagtgaag gtcgccgtga agcactgggt gaactgctcg
    26041 cgcgaattaa aattgctcct caacgcatta tgcgtcttga tgaagccagc gaccgtgggc
    26101 gttatctgat gattggcgct gccgaacatg gttttgtcga tacggtgcgt aatctggcgc
    26161 tgatctgcga aagcgatctg ctcggtgaac gcgttgcccg ccgtcgtcag gattctcgcc
    26221 gcgccatcaa ccccgataca ctgatccgta acctcgcgga gctgcatatt ggtcagccgg
    26281 tggtccatct ggagcacggt gtcggtcgtt atgcaggaat gaccacgctg gaagcgggcg
    26341 gcattactgg cgagtacctg atgctcacct atgccaacga cgccaaactg tatgttccgg
    26401 tgtcgtcact gcatctgatt agccgttacg caggtggcgc ggaagaaaac gccccgctgc
    26461 ataaacttgg cggcgatgcg tggtcacgcg cgcggcagaa agcggcggaa aaagtgcgtg
    26521 atgtggcggc agaattactg gatatttacg cgcaacgcgc cgccaaagag ggcttcgcgt
    26581 ttaaacacga tcgcgagcag tatcagttgt tctgcgacag cttcccgttt gaaaccacgc
    26641 cggatcaggc acaggccatt aatgcggtac ttagcgacat gtgtcagccg ctggcaatgg
    26701 atcgtctggt gtgcggcgat gttggctttg gtaaaacaga agtggcgatg cgcgccgctt
    26761 tcctggcagt agataaccac aagcaggtgg cggtgctggt gcctaccacc cttctcgcgc
    26821 agcagcatta cgacaacttc cgcgaccgtt tcgccaactg gccggtacgt atcgaaatga
    26881 tctcccgttt ccgcagcgcc aaagagcaga cgcaaatcct tgcggaagtg gcggaaggga
    26941 aaatcgatat tctgatcggt acgcacaaac tgctgcaaag tgacgtcaag tttaaagatt
    27001 taggcctgct gattgtcgat gaagaacacc gcttcggggt gcgtcataaa gagcgcatta
    27061 aagcgatgcg tgcgaacgtg gatattctga cgcttaccgc tacgccgatc ccacgtacgc
    27121 tgaatatggc gatgagcgga atgcgcgatc tgtcgattat tgccacgccg cccgcccgtc
    27181 gtctggcggt caaaacgttt gtccgcgagt atgacagtct ggtggtccgg gaggcgatcc
    27241 tgcgtgaaat tttgcgcgga ggacaggttt attatctcta caatgatgtg ggaaacatcc
    27301 agaaagccgc cgaacggctg gcagaactgg tgccagaagc gcggatcgcc atcggtcacg
    27361 ggcagatgcg cgagcgcgaa ctggaacggg tgatgaatga tttccatcat caacgtttca
    27421 acgtgctggt ttgtacaacc attatcgaaa ccgggatcga catcccgaca gccaacacca
    27481 ttatcattga acgcgcggat cacttcggtc tggcgcagct gcaccagtta cgcggtcgcg
    27541 tcggacgttc gcatcatcag gcatatgcat ggctgctgac gccgcatcca aaagcgatga
    27601 ctaccgatgc acaaaaacgt cttgaagcga ttgcctcgct ggaagatctc ggggcaggtt
    27661 ttgcgctggc aacgcacgat ctggagattc ggggcgcggg tgaactgctt ggcgaagaac
    27721 aaagcggctc aatggaaacc atcggtttct cgctgtatat ggagttgctg gaaaacgccg
    27781 tcgatgcact gaaagccgga cgcgagccgt cgctggaaga tctcaccagc cagcaaacag
    27841 aagtcgagct gcggatgccg tcgctattgc cagatgattt cattcctgac gtgaatacgc
    27901 gtttgtcgtt ctataaacgt attgccagcg ccaaaacgga aaacgaactg gaagagatca
    27961 aagttgagct tatcgatcgc ttcggcctgc tgccggatcc ggcgcgtacc ctgctggata
    28021 ttgcccgtct gcgccagcaa gcgcagaaac tggggatcag gaagctggaa ggtaatgaga
    28081 aaggcggcgt gatcgaattt gccgagaaga atcacgttga tccggcctgg ttgattggtt
    28141 tgctgcaaaa acagccgcag cattaccgcc ttgatggtcc gacgcgcctg aagtttattc
    28201 aggatttgag tgagcggaaa acgcgtatcg aatgggtacg ccagtttatg cgtgaactgg
    28261 aagagaacgc catcgcttaa tggtaaatcg ggggcgtttc tgcgccccca tctgcaacat
    28321 ttacaaattc tttgcacttc cctgcactat ccgacactgt caccatccat aattcaggct
    28381 tatctgttta ttacaataac cttatattta ttatggattt ttggtgatga tcaaaacgcg
    28441 tttttctcgc tggctaacgt tttttacgtt cgccgctgcc gtggcgctg
//
LOCUS       DAPOZO010000063        28138 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPOZO010000063 DAPOZO010000000
VERSION     DAPOZO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.063"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..207
                     /locus_tag="ROI21_002971"
     CDS             <1..207
                     /locus_tag="ROI21_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDX5111871.1"
                     /translation="RGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQDIPGV
                     LAVFAERRKDSFGPYVRLMSVTLN"
     gene            281..649
                     /gene="cbtA"
                     /locus_tag="ROI21_002972"
     CDS             281..649
                     /gene="cbtA"
                     /locus_tag="ROI21_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HDX5111872.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            738..1111
                     /gene="cbtA"
                     /locus_tag="ROI21_002973"
                     /pseudo
     CDS             738..1111
                     /gene="cbtA"
                     /locus_tag="ROI21_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
     gene            1108..1302
                     /locus_tag="ROI21_002974"
     CDS             1108..1302
                     /locus_tag="ROI21_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111873.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            1315..1428
                     /locus_tag="ROI21_002975"
     CDS             1315..1428
                     /locus_tag="ROI21_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDX5111874.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(1717..1863)
                     /locus_tag="ROI21_002976"
                     /pseudo
     CDS             complement(1717..1863)
                     /locus_tag="ROI21_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1917..2099
                     /locus_tag="ROI21_002977"
     CDS             1917..2099
                     /locus_tag="ROI21_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDX5111875.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(2200..2529)
                     /locus_tag="ROI21_002978"
     CDS             complement(2200..2529)
                     /locus_tag="ROI21_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDX5111876.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(2701..3759)
                     /locus_tag="ROI21_002979"
     CDS             complement(2701..3759)
                     /locus_tag="ROI21_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDX5111877.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(3957..4430)
                     /gene="sbmC"
                     /locus_tag="ROI21_002980"
     CDS             complement(3957..4430)
                     /gene="sbmC"
                     /locus_tag="ROI21_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDX5111878.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(4549..5715)
                     /gene="dacD"
                     /locus_tag="ROI21_002981"
     CDS             complement(4549..5715)
                     /gene="dacD"
                     /locus_tag="ROI21_002981"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDX5111879.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAVNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            5924..7351
                     /gene="sbcB"
                     /locus_tag="ROI21_002982"
     CDS             5924..7351
                     /gene="sbcB"
                     /locus_tag="ROI21_002982"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDX5111880.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEATFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNVVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(7394..7621)
                     /locus_tag="ROI21_002983"
     CDS             complement(7394..7621)
                     /locus_tag="ROI21_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDX5111881.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(7635..8693)
                     /gene="yeeE"
                     /locus_tag="ROI21_002984"
     CDS             complement(7635..8693)
                     /gene="yeeE"
                     /locus_tag="ROI21_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDX5111882.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(8872..10230)
                     /gene="plaP"
                     /locus_tag="ROI21_002985"
     CDS             complement(8872..10230)
                     /gene="plaP"
                     /locus_tag="ROI21_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDX5111883.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(10220..10282)
                     /gene="yoeI"
                     /locus_tag="ROI21_002986"
     CDS             complement(10220..10282)
                     /gene="yoeI"
                     /locus_tag="ROI21_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDX5111884.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(10497..11426)
                     /locus_tag="ROI21_002987"
     CDS             complement(10497..11426)
                     /locus_tag="ROI21_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5111885.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTHTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(11472..12296)
                     /locus_tag="ROI21_002988"
     CDS             complement(11472..12296)
                     /locus_tag="ROI21_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX5111886.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDVDALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(12379..12633)
                     /gene="yoeB"
                     /locus_tag="ROI21_002989"
     CDS             complement(12379..12633)
                     /gene="yoeB"
                     /locus_tag="ROI21_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDX5111887.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(12630..12881)
                     /gene="yefM"
                     /locus_tag="ROI21_002990"
     CDS             complement(12630..12881)
                     /gene="yefM"
                     /locus_tag="ROI21_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDX5111888.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            13164..13214
                     /gene="hisL"
                     /locus_tag="ROI21_002991"
     CDS             13164..13214
                     /gene="hisL"
                     /locus_tag="ROI21_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDX5111889.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    13190..13313
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            13360..14259
                     /gene="hisG"
                     /locus_tag="ROI21_002992"
     CDS             13360..14259
                     /gene="hisG"
                     /locus_tag="ROI21_002992"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDX5111890.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRCAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            14265..15569
                     /gene="hisD"
                     /locus_tag="ROI21_002993"
     CDS             14265..15569
                     /gene="hisD"
                     /locus_tag="ROI21_002993"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDX5111891.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            15566..16636
                     /gene="hisC"
                     /locus_tag="ROI21_002994"
     CDS             15566..16636
                     /gene="hisC"
                     /locus_tag="ROI21_002994"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDX5111892.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            16636..17703
                     /gene="hisB"
                     /locus_tag="ROI21_002995"
     CDS             16636..17703
                     /gene="hisB"
                     /locus_tag="ROI21_002995"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDX5111893.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            17703..18293
                     /gene="hisH"
                     /locus_tag="ROI21_002996"
     CDS             17703..18293
                     /gene="hisH"
                     /locus_tag="ROI21_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDX5111894.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            18293..19030
                     /gene="hisA"
                     /locus_tag="ROI21_002997"
     CDS             18293..19030
                     /gene="hisA"
                     /locus_tag="ROI21_002997"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDX5111895.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKNLVAGINVPVQVGGGVRSEDDVAALLEAGVARVVVG
                     STAVNSPERVKGWFERFGADALVLALDVRIDEQGNKLVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            19012..19788
                     /gene="hisF"
                     /locus_tag="ROI21_002998"
     CDS             19012..19788
                     /gene="hisF"
                     /locus_tag="ROI21_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDX5111896.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            19782..20393
                     /locus_tag="ROI21_002999"
     CDS             19782..20393
                     /locus_tag="ROI21_002999"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDX5111897.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(20491..21468)
                     /gene="wzzB"
                     /locus_tag="ROI21_003000"
     CDS             complement(20491..21468)
                     /gene="wzzB"
                     /locus_tag="ROI21_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDX5111898.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLRVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(21614..22780)
                     /gene="ugd"
                     /locus_tag="ROI21_003001"
     CDS             complement(21614..22780)
                     /gene="ugd"
                     /locus_tag="ROI21_003001"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDX5111899.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTVAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(23029..24435)
                     /gene="gndA"
                     /locus_tag="ROI21_003002"
     CDS             complement(23029..24435)
                     /gene="gndA"
                     /locus_tag="ROI21_003002"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDX5111900.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDMILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLYGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKDGVFHTEWLD"
     gene            complement(24530..25549)
                     /gene="galE"
                     /locus_tag="ROI21_003003"
     CDS             complement(24530..25549)
                     /gene="galE"
                     /locus_tag="ROI21_003003"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDX5111901.1"
                     /translation="MKLLVTGGLGYIGSHTVVSLLDAGYEVIIIDNLSNSHLSVLKRI
                     NNICLRKASFIEGDIRNRTVLNQIFNSYNIDAVIHFAGLKSVDQSISSPISYYDNNVV
                     GTLALLEEMLRHDVMRFIFSSSATVYGSPKSIPIKESNEVGHTTNPYGTSKLIIEKIL
                     SDICYSNSSFRCTILRYFNPVGAHTSGLLGEDPRGKPNNLMPYICQTAIGKLNEVHIF
                     GNDYNTHDGTGVRDYIHVMDLAEGHVAAVKASNEGENCKIYNLGTGKGYSVLDLIKTF
                     EQITNVAVPHIFVGRRPGDIAECWSDPSKAYEELNWRAKKDLADMIRDAWKWQVQNPN
                     GYSDD"
     gene            complement(25588..26298)
                     /locus_tag="ROI21_003004"
     CDS             complement(25588..26298)
                     /locus_tag="ROI21_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015378195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDX5111902.1"
                     /translation="MPAYNASATIEQAVNSVIDQDFKDWHLYIIDDASHDNTFELAGK
                     FQDERITVIKNRNNKGVAESRNIALNICKGNYVAFIDSDDMWIKNKLSLQMEKLEDGW
                     DLVCSNFITFSNEKEYINARISPEIITYDMMLKSNFIGNLTGIYNREKLGIFNQVNEG
                     HEDYIMWLDIISVTNRAYCIQECLAKYRVNEHSLSSNKIKAIAWQWNIYRKNRRLSFL
                     HSIKYIIYYFFHALLKRV"
     gene            complement(26329..27381)
                     /locus_tag="ROI21_003005"
     CDS             complement(26329..27381)
                     /locus_tag="ROI21_003005"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDX5111903.1"
                     /translation="MKNKISFIISDITNKGGTERVTALLSNILSSNIENAEVEIFSLQ
                     KKHANPFYFLSNKISVKYGNGWRYYSLLSYLIFCKKNKHKVIVESMGRLSLECGILAK
                     AIRLHDIYFHEHVGLNSFKKAIQLIKIFSYYIAKKVIVLTSHDYNLLTTKYGIKNVIQ
                     IPNINPFDTSNNIIKPYDQRENIVIAVGRFTEQKNFNELIEIWHEANIPNWTLYIIGD
                     GPNKRILEKAIAGMSSIKMFKTTDEICNYYNAAKIYAMTSLYEGLPMVLIESQSFGIP
                     SVAYDCPTGPAEIITDNVNGFLIPFKNRELFIQKLRKLTSDDNLSNIFSTNAIKNSRK
                     YNADAIIHKWISLLGI"
     gene            complement(27378..>28138)
                     /locus_tag="ROI21_003006"
     CDS             complement(27378..>28138)
                     /locus_tag="ROI21_003006"
                     /inference="COORDINATES: protein motif:HMM:NF025017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDX5111904.1"
                     /translation="DSRIKIIYNKENQKIVKSLNQGLLLAKGQYIARFDGDDIAAPDR
                     IEKQLHYLEANDLDIVGCQMHSINEIGEHLTKSISPVGEDIVKKVSKYVSPIAHIWLA
                     KREVYDELQGYREIPYAEDYDFILRALDRGYKCDNHPEYLMYIRHRQGNSASVASLLQ
                     RKAHNYVLKLHKLRIRRGRDNFDVLSMHKKLSSSILIEKLHSTSSRCLALAVQQKSMY
                     RRFFFIFLTVLLSIYNMQYLYRRLIVRNIMRNKK"
BASE COUNT         7499 a         6951 c         6488 g         7200 t
ORIGIN      
        1 cgtggtgaag ccatgcgtat tcaccaacaa catccgacat cccgtctttt tccgttctgt
       61 accggtaaat accgctggca cggcagtgct gaagcgtata ccggtcgtga agtgcaggat
      121 attcccggtg tgctggccgt gtttgctgaa cgccgtaagg acagttttgg tccgtatgtc
      181 cggctgatga gcgtcaccct gaactgagtg ggaattctga tgagcagaat tatcgccact
      241 accggaccat tcttagccga ttttctgtaa ggattttatc gtgtcagaca cactccccgg
      301 gacaacactt cccgacgaca atcacgaccg cccctggtgg gggctgccct gcaccgtgac
      361 gccctgtttc ggggcacgtc tggtgcagga gggtaaccgg ttgcattacc ttgccgaccg
      421 cgccggtatc agaggcctgt tcagcgatgc agatgcgtac cacctggacc aggcctttcc
      481 gctgctgatg aaacaactgg aactcatgct caccagcggt gaactgaatc cccgccatca
      541 gcataccgtc acgctgtatg caaaagggct gacctgcaaa gccgataccc tcagcagttg
      601 tggttacgtt tatctggctg tttatccgac gcccgaaatg aaaaattaac tctccagaat
      661 agccttctgc tacggcctgg tgttttcacc acgccacttt tccattttta tatctgcata
      721 tcaggaaaat cttcagtatg aaaacattac ctgtattacc cgggcaggcg gccagttctc
      781 gcccgtctcc tgttgaaatc tggcagatac tgctgtcccg actgctggac cagcactatg
      841 gcctcacact gaatgacaca ccttttgccg atgaacgtgt gattgagcag catattgagg
      901 caggcatttc actgtgtgat gcggtgaact ttctcgtgga aaaatacgcg ctggtgctac
      961 cgaccagccg ggattcagcg cctgtacccg ctctcagtta ataaacagca tcgatatcct
     1021 ccgggctcgc agggcgaccg gcctgatgac ccgcgacaat tacagaacgg taaataacat
     1081 taccctgggt aagtatccgg aggcgaaatg atgacactgg aagccgacag cgttaacgta
     1141 caggcgctgg atatggggca cattgtcgtt gacattgatg gtgttaatat cactgaactg
     1201 attaataagg ccgctgaaaa cggttattca ctccgcgtgg tggatgaccg tgactctacc
     1261 gaaacaccgg caacttatgc cagccctcac cagttgctgt aagacaatgc agtgatgcag
     1321 ggtatagtgc cattaaccac agaaacagca ctgttgtcct gtattaagca ggcaagagaa
     1381 gatgtcctta ccctgcgcca tctgcagctt ctgcaccaga accggtgaat ctcactcaac
     1441 aggcttcatt tcccctgacg ccagcctgaa tacagctggc gttttcattt ataaacagaa
     1501 aggaaaaccg ataataatgg aatggacgcc ccgaccatga agcaacggta ggatattact
     1561 gataaacctt ccagtggagg tgcgttatgg ccgttttaac gattagcatt gacctgacaa
     1621 aaaatgaatt ccaaatccac ggtctgggta ggaacagaaa aatctaagct cagaaaacgg
     1681 attaaagtaa gcgtcaacgg agcaccgtat tgacgcttat ttattggtga gaactacgtt
     1741 ccatggcagg agttcgtcaa cacggttgga gggccattcc ggcagtatgc tcagaatatg
     1801 gcgcagatac gcttccggat cgataccgtt cagacggcag gtgcctgctt ttccggcaac
     1861 aagcaggaat ctcagcgctg aagcaggaat tagaggtaaa aacgccctac cgggccatga
     1921 atcacccggt cattggagta gtaaccaaag cagatttagc cagcatggaa caaatatctt
     1981 tggtgaaaag ttggttacgg gaagctggag cgcacaacgt gttagtaacc agtgcagtta
     2041 ataacaatgg agttacagaa ctttttgcct tgctgcatac agaagaaggc tgttgttaat
     2101 ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat caatgagcaa
     2161 tgagggttgc cgggcaaccc tcattgaata aaacgggaat tacttcgctt cgccgttttt
     2221 catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg agagctcggc
     2281 atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc tggcgatgat
     2341 gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt tgtccagcaa
     2401 caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt cacgttgcag
     2461 tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg aaggcttggt
     2521 agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc aaccgctatt
     2581 cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc ccttttcact
     2641 atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga agttcaggaa
     2701 ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat gtgccgtttc
     2761 catgttcagc cacacataac catagattgg tgtttccaca accttcaggt catggtggtt
     2821 atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat tggcaaaaac
     2881 cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt gctgcatcat
     2941 gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaatggctgg ggcgcgtggc
     3001 ccagtacgca ttgatttgca actccagcat acaaaccaga ttgcggttaa tggtctggat
     3061 accttcatat atcgatttgg gaatacgggt ttctttgctg gcgggcgcaa tcagcccacg
     3121 cattttcacg gcatcggtca gcagtttttg tagatggctt tccagacgtg ggcgttcgag
     3181 taagttcggt gagaatgcag attgatagac ccgattatac tcggtcagac ttttcgccag
     3241 ttgaatgcgc cagtggatga acgcccgttg tggccagata ccggtaaaca acattgccag
     3301 taaagagccg aggatcacat cgccgcttcg ccataacgcc gtatcaattt cacctgtcgg
     3361 ggaacccaca acaattgcca gcgtcacccc aatcaataat ccttgatacg gtttcttgcc
     3421 cagcgccagc caaccgcaaa gaaacatcgc cgccgcgcac cagactaaca tcagcggtaa
     3481 cgaaattaac tccagttgca gagcgataag acctaaaatc gaccccaaca ccgtaccgcc
     3541 aatacgttcg aaggcacgag ggacaacgtt cccccagaac gagattggcc ccataatcac
     3601 caccatggtg accagcggcc aggtgctttc cgggatagta aacaggcgga taatgagaaa
     3661 agtgagcagg aatgccagcg cgacccgagt accatgcaca atgcggtaat ggcggtatac
     3721 ccggatttca aacgggctta atgacttatc ggcacgcact cagaaactcc gtaatgaaga
     3781 tgaaaagcgc tcataacaca tctgtcggcc agaaggcgcg cctctgagtt atccgacgga
     3841 cataccggga acagtgtaat ttcccggcag gagcgaaaaa tttgaaaggc gtatcttact
     3901 cttttcgctt tcaaaaaaag agtggtcttc gcgttaacac accgccctga gatgaattag
     3961 tgatgttttg gctgcaccgc aacatacatt tcgatatccc agtacccatc ttccgcgcca
     4021 ttgttcaagt aaacctcgaa gcagggcttt ggtaacattt cataagcact gtcctgcaag
     4081 aggctattaa agaactgata ccagggttta gcaaaatcat caccgactac acgagccacc
     4141 gccaccgcat attgaccacc tgaaatttct gtcagaatga cgccctcact gttttcgggg
     4201 agcgtaaagt tattcggtac cgtcacgacg gtgtcgcagc gtaatttttc ggcgggtgtt
     4261 tcatccggat tgtcgtaata gacagcaacc cactccttcg gcacaatatt tttgctatct
     4321 acccacatca tcaactgctc aaaacctttc tttaccgtct gttcccacgg gccaacgaga
     4381 tggaaacctg caacggtacg tttctcttcc tgcttaatct cgtagttcat gacgcctcca
     4441 ttgatgctgt ttttatatac agtatagttg caaaattaaa accacaagga atgagtgttg
     4501 attatgcgag cagactcgca ctcctgccag tctgctgcaa aagaaaggtc aggccttatg
     4561 gtggaaataa tcactcaggc gagaaaacat gctgccttcc ccgacagatt ccagggtaac
     4621 cagcggccag tgcgccacct gtttatcacg gtcgtaaagt tcaatttccc ctacccgctg
     4681 atgggcgcta attggcgcgg tgagctcttt accatcaagg gtatatttgg ctttgatatg
     4741 tggaatttcg gctttcggta gcaccatcca gaactcttgt tccgttccca ggtcgatatt
     4801 ttctttatcg ccataccaga tgcgttccgt tccgaccttt ttcccacggt gcaaaatttg
     4861 caccgtagta aagttttgtt gcccccaacg cagtaatttt cttgcctctt cctcacgacc
     4921 ttttgcactg tcagccccca ttaccactgc aatgagacga cgctgcccat ctacagccga
     4981 agcaatgaga ttaaacccgg caccagaagt atgacccgtt ttcaggccgt caacattcat
     5041 ggttttatcc cacaacaacc cgttacggtt ttgctgggtg ataccgttcc aggtgagact
     5101 tttctcactg tacatatgat aaaactcggg ctcgccgtgg atgatagcgc gagaaagcac
     5161 agctaaatca taagccgagc tatgctggcc aggtgcatcc agaccatgca ctgtttcaaa
     5221 atgcgtatcc ttgagatgca gcttctcggc atagttgttc atcatttcaa caaactgccg
     5281 ttgcccaccg gcaatatagt cagccagagc aacacaagcg tcatttccgg aatccacaat
     5341 taaaccacgg cttaaatcac gtaccgatac gcgatcgccc tctttcaaaa acatcagtga
     5401 agaaccgaca aacaccggat tatctttcgc ccacgcatcg cgccccacgg tgacaatatc
     5461 gtctggcgta atgcgatgac tatcgatagc gcgatccacg acataacccg tcatcagctt
     5521 tgtcaggctg gcgggattgc gctgttgatg ctcattaacc gcggtgagga tctgaccggt
     5581 ggtgtaatcc atcaataccc aggacccggc atgaatctct ggaggctgag gtgaaaaagg
     5641 aatgttttcc gccgcaaaac cagacgataa gttaaaaacg aacaaagaag cagcaataat
     5701 aagacggcgt ttcaacagca aaccctcagg agtttcaaat agctgttctt tttacggaaa
     5761 tacttatgaa ctggctggaa taaagtgcaa gaaaatgtga ctaccctctc atttttatct
     5821 gacatgatct gttgccactc gctgccaaat tgtggcgcta aagctgatta gcacggtgat
     5881 atttgatact ctggcagaca gcagaaataa cggatttaac ctaatgatga atgacggtaa
     5941 gcaacaatct acctttttgt ttcacgatta cgaaaccttt ggaacgcacc ccgcgttaga
     6001 tcgccctgca cagttcgcag ccattcgcac tgataacgaa ttcaatgtaa tcggcgaacc
     6061 cgaagtcttt tactgcaagc ccgcggatga ctatttaccc cagcctggag cagtattaat
     6121 taccggtatt accccgcagg aagcacgggc gaaaggagaa aacgaagcca cgtttgccgc
     6181 ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca acaatgtgcg
     6241 tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc cttacgcctg
     6301 gagctggcag catgataact cgcgctggga tttactggat gttatgcgtg cctgttatgc
     6361 cctgcgcccg gaaggaataa actggcctga aaatgatgac ggtctaccga gctttcgcct
     6421 tgagcattta accaaagcga atggtattga acatagcaac gcccacgatg cgatggctga
     6481 tgtgtacgcc actattgcga tggcgaaact ggtaaaaacg cgtcagccac gtctgtttga
     6541 ttatctcttt acccatcgta ataaacacaa actgatggcg ttgattgatg ttccgcagat
     6601 gaaacccctg gttcacgttt ccggaatgtt tggggcatgg cgtggcaata ccagctgggt
     6661 ggcaccgctg gcgtggcatc ctgaaaatcg caatgtcgta attatggtgg atttggcagg
     6721 agacatttcg ccattactgg agctggatag cgacacattg cgcgagcgtt tatataccgc
     6781 aaaagccgat cttggcgata acgccgccgt tccggttaag ctggtgcaca tcaataaatg
     6841 tccggtactg gcccaggcga atacgctacg cccggaagat gccgaccgac tgggaattaa
     6901 tcgtcagcat tgcctcgata acctaaaaat tctgcgtgaa aatccgcaag tgcgtgaaaa
     6961 agtggtggcg atattcgcgg aagccgaacc gtttacgcct tcagataacg tggatgcaca
     7021 gctttataac ggctttttca gtgacgccga tcgtgcagca atgaaaattg tgctggaaac
     7081 cgagccgcgt aatttaccgg cactggatat cacttttgtt gataaacgaa ttgaaaaact
     7141 gttgttcaat tatcgggcgc gcaacttccc ggggacgctg gattatgccg agcagcaacg
     7201 ctggcttgag caccgccgcc aggtcttcac gccagagttt ttgcagggct acgctgatga
     7261 attgcagatg ctggcacaac agtatgccga tgacaaagag aaagtggcgc tgttaaaagc
     7321 gctttggcag tacgcggaag agatcgtcta atttgaagcc tccgccgctg gtacggcgga
     7381 ggacaatgtt gagtcaggct ttttgaacgg tgatgctcca cgctgcatcg ccaatctgct
     7441 gataatcggt gatggcatgt ccttcttctg ccgcccactg tgggattgct tcagttgcct
     7501 gggtgcaatc aaactcaatt accaactcat cgccgctggc catttctgcc agtgctgctt
     7561 ttgcttctat gagtggaaac ggacagacct gcgtcaccac atccagcttt ttaatcgcca
     7621 tctttacacc ttacttaatt tgccgcagca gttgccagtc gcgccttacg ctgcggtcga
     7681 acatacacaa gccaggacgc agtccagact ccgagaatca taaatacaag gccaatccag
     7741 ccctgccagg tcatcattgc agtcatgacc agtccattac cgatagagca accacctgca
     7801 atgctggcac cgaagcccat cagtacacca cctaacccgc tacgtaatgt tgtttgtgca
     7861 tcagcagcgc gaacgcggaa ctcacggctc gctttggcgg caataaatga ccccacgaag
     7921 atccctaaca ccaggaaaac gccccagtta atgtatttca cgtcacccgc gaccagaaat
     7981 tgcagaatat tggccgttgg ggaagtgatg cctaacccaa acatacgtcc ggttgcttca
     8041 ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta cagctgtgac aaagggatgc
     8101 cagcgttttt caaacagaat atgagcgatc ccggttcggc gcggcggtaa ggtcgcgact
     8161 ttgagttttg gcttcttcaa ctctttcatc accacccaga gcgttatcac cagcaaaacg
     8221 gcaaccaacg gccacacaga caaattaaaa gtatcagcaa tagagttatg ttcagtagtg
     8281 tagtgctgca aggtttgatt taaaccactg gcgtgtggag aacgcatcac cgcactcatc
     8341 accatataag tgaaaagcgc gatccaactg ccgatcaatc cctcacccgc gcgataccag
     8401 gtcccggtgg cacatccacc cgccagaaca attcccagcc cgaaaagata cccacctata
     8461 acagtaccaa gccacgggaa cgccccggct tcgtaagtca gtaggcccgc ctgaattaac
     8521 gcaaaaaccc ccacgctttg taccgaaatt gcaatcagca aggcgtaaaa catgcgatta
     8581 tttttcgcga tatacatatc gcgaaaacca cctgtcaggc aaaaacgccc acgctgcatg
     8641 acaaatccca gcagagcacc acaaattagc ccgcttaata tcattgaaaa cataaattaa
     8701 ttaaccagat gaatgttaat gaggaaatta ttcatgactg gtggaatgca gaccaataac
     8761 caaattctct aaattagaac aaatggttat taatgaggat ttcgataaaa aagaataaaa
     8821 aaaccggagc caaatattca ttcgactccg gttgattatt agaaaagatg attatgctac
     8881 gtcttcgtac tgcggaaccg ggttacggaa gctcttggtg acgcaagcca ggtaaatcag
     8941 gccgatagcc gcccagatca gacccagaac cattgagctt tcttccaggt taacccacag
     9001 cgcaccaacg gtcagcgcac cacacatcgg caggaacaga tactggaagt gatctttcag
     9061 cgtcttgtta cgcttctcac ggatccagaa ctgcgagatg accgacaggt taacgaaggt
     9121 gaacgccacc aacgcaccaa agttgatcag cgccgtagcc attaccaggt cgaagttgat
     9181 tgccagcagg gcaatcgcgc caaccaggat gatgttcatt gccggagtac gccatttcgg
     9241 gtgaacataa ccgaagaagc ttttcgggaa tacgccgtca cgccccatta cgtacatcag
     9301 acgcgcaacg cctgcatgcg ccgccatacc ggacgccagt acggtaatgg tggagaagat
     9361 cagcgcacca acctggaatg ctttacctgc aacatacagc atgatttcag gctgtgacgc
     9421 atccggatct ttaaagcgag agatatccgg gaagtacagc tgcaggaagt aagttgcaaa
     9481 gatgaagatc atgccgccaa tcagcgcggt caggaaaatc gcacgcggga tcacgcgctc
     9541 tgcatctttg gtttcttccg acaggttgct gatgccgtca aagccggtaa aggagaagca
     9601 caggattgtc gccccggtaa tcatcgggat aacatgcgca tcgccagacc agaacggacg
     9661 agtgctcgcc agcgtaccag cgccttcacc ttcaaatacg ccataaacaa ccatgcccag
     9721 aatcaccgcg atcagcacta cctgcaacac gacgattaca gtgttgaagt tcgctacgga
     9781 tttcagacta cgcaggttaa aggcggtcat aaaggccacc agcgccacca caaacatcca
     9841 cgatgggatg gaaggcacca gagcttcaaa atagattttc gccagcagaa tgttgatcat
     9901 cggcgcgaac agatagtcga gcagagaaga ccaacccacc ataaagccga cagtcgggct
     9961 aatggatttc tgggcgtaag tgtatgcaga gccagcagaa ggatagcggc gaaccagctt
    10021 cccgtagctc agagccgtaa acaggatcgc aatcaatgcg aacgcatagg ctgtcggcac
    10081 atgaccatcc gtgaggcctg aaacgatacc aaaagtatca aacagcgtca tcggctgcat
    10141 ataggcaaga cccatcatta caaccggaac taacgtaagc gttttacgca attccacgcg
    10201 agaggtgttt ggagtaacgt tatgcgacat ggtcattctc ctttacggtg ataaccgtcg
    10261 cgtaagcaaa aaattgcccc atttttttgg attcctcagc gacaacaact gtcgattttt
    10321 agtaaatatc tatccggtac gaagcccggc ctcttggtat gaatgattgg tttgaagcaa
    10381 aaaataaccg acgctgatga aacgtcggtt tttagtcatt ttttgacagc ggcgcattgt
    10441 gcctaaatgg gggggaaatg acaagagaat gagaggcttg tcataataat ttttctttaa
    10501 atggctgatt ttccgtcatc agattttgca taaacaccgg cgatcgcact atttgctaaa
    10561 atttcatctc gccaccagcc gctggcaagc cctgcggtct gttcgttcca gccaatccag
    10621 acgggttcaa agagagtttg ggcgactact ttcttctcga ttaacgcgcc actatcgaga
    10681 aaacgttgcg ccagataacg gggtaaatag ccgcagccaa ggccgctaat ttgcagctcc
    10741 agcttggttt taaaatcgaa aacggtaatg gcttcttgtt cgtcgagaag ttgtgaggcc
    10801 gtagaagcgc cggcctgtgc agtgtccccc accacaatcg cccgatatcg tttaatgata
    10861 cgacggttta acggttcttc ttctagggct aacggatgat gaggcgcgac cgcaaagact
    10921 tgttccagat cgcccaaccg cgagaaacca aactcactgg aagatggtgg ctcatgcatc
    10981 gcgccaacaa taatatccgc tcgcccctga gtcagggcat cccaggaacc accgagtacg
    11041 ccattgataa acttcaggcg cgtcacgcta tgatgttgat aaaaagcttc aatcagtggc
    11101 gcaagcagag aaaaaggaaa cgtgtcgtcc acgccaatca ccagctcgtt ttcccagcct
    11161 tcatgcagtt tgatcgcctg cttttccagt tctcgcacgg tatgcagaac ttcccgccct
    11221 ttctctaata acatttttcc ggtatgggtg aatttagcgc ggtgaccgct acgatcaagc
    11281 aattgaatat taaggtcgct ttccagcttg tgaacggtgt aactcagagc ggacggcgtc
    11341 ttatagagtt tggccgacgc cgccgcaaaa ctgccttctt tttctaaggc atcgaggatc
    11401 atcaacacat ccagcagtgg tttcatacac gcccccttgc agtgcatgcg gcgtcctgct
    11461 ggcgtgatcg tttactccag cggcattacc agcggatcgg gatactggta ttcaaatccc
    11521 agttcattac aaatccgact gccatcaata atcttacctt tgccgctgtc cagactattt
    11581 ctgaactgcg gaggctccag ccccagtaaa cgggccatct gcggatagaa aacattacgc
    11641 gcagggtgag ctggcgcaca tatattatag atgtgtccgc ctttaggtgc ctgtaacaac
    11701 agagtgatag cgccaataac atcttctaaa tggactaaat taacaccatg ttcaccatca
    11761 ggcgcggttt ttccggcaaa gaagcgtccg ggatgacgtc ccggtccgac cagccccgca
    11821 agacgcagaa tatcgaccga agtaccgggt aaattgtgca gccagtcttc gagttcttct
    11881 aatactcgtc cactgttggt taccggatta cgcggggtag tttctttcac cgtgccttgc
    11941 gcgtcgccat agacagatgt tgagctggta aaaataatgc ggggaatacg atgggccagc
    12001 gcgctatcca ctaactcttg taccgcttgt aaatagaact catcgccggg gccgctacga
    12061 cgtgccggaa gcgtaatgac cagcgcatcg acatccatca gggcatccag atcgtcagaa
    12121 tcgcaaacta actcaggctc catgcgaagc agatagctat caatgccact cattcgggcc
    12181 gcttcgacac catcttgtgt ggttttactc ccggtgactt gccagcctcg cgctgaaagt
    12241 gacatcgcca gcggcatgcc taaccaccct aacccgacaa ttgcgacctt tttcatccgt
    12301 tatctcctga tacaccttag atctataagg ctacgctagc gtatcaaaac tgacaattca
    12361 ttctatgaat gaatctgttc aataatgata acgacatgct gcaatgagca gtgaatcgtc
    12421 ggtaaccgcg tataccagac ggtgctcctc tgtaatgcgt cgggaccaga aacctgacaa
    12481 attatgtttc aggggttctg gcttcccctt accttcaaat ggcgttctgc gggtatcttt
    12541 gataagttca ttgatctttt taacaattcg cttatctgtt tcctgccagt acagataatc
    12601 atcccatgat tcctcagacc agattagttt cactcaataa tgtccttttc tgttcctttg
    12661 cctgatttca ggctatcgat tgagtccatc aatctccggg cgttagcggg ggagcgcagt
    12721 agataagccg tctcttccag cgagttgtat tcttcgagtg acatcagaac acaagcctct
    12781 ccattctgac gagtaataag gatcggggca tgatcttcaa cggctttcat cattgttgcc
    12841 gacaaattct gacgcgcttc gctgtagcta attgtacgca tgtcaatctc ctcttttgta
    12901 cagttcattg tacaatgatg ggcgttaatt aactatttat taattagttt gtagatcaag
    12961 gtattgtcag tgagacgaaa atccaggctt cgctattttt ggtgccgtca gcttagagga
    13021 cagtcctctt agccctctcc tttccccgct cattcattaa acaaatccat tgccataaaa
    13081 tatataaaaa agcccttgct ttctaacgtg aaactggttt aggttaaaag acatcagttg
    13141 aataaacatt cacagagact tttatgacac gcgttcaatt taaacaccac catcatcacc
    13201 atcatcctga ctagtctttc aggcgatgtg tgctggaaga cattcagatc ttccagtggt
    13261 gcatgaacgc atgagaaagc ccccggaaga tcaccttccg ggggcttttt tattgcgcga
    13321 ttgataacgg ttcagacagg tttaaagagg aataagaaaa tgacagacaa cactcgttta
    13381 cgcatagcta tgcagaaatc cggccgttta agtgatgact cacgtgaatt gctggcgcgc
    13441 tgtggcatta aaatcaatct tcacacccag cgcctgatcg cgatggcaga aaacatgccg
    13501 attgatattc tgcgcgtgcg tgacgacgac attcccggtc tggtaatgga cggcgtggta
    13561 gatcttggga ttatcggcga aaacgtgctg gaagaagagc tgcttaaccg ctgcgcccag
    13621 ggtgaagatc cgcgctactt taccctgcgt cgtctggatt tcggcggctg ccgcctttcg
    13681 ctggcaacgc cggttgatga agcctgggac ggcccgctat ccttaaacgg taaacgtatc
    13741 gccacctctt atcctcacct gctcaagcgt tatcttgacc agaaaggcat ctcttttaaa
    13801 tcctgcttac tgaacggttc tgttgaagtc gccccgcgtg ccggactggc ggatgcgatt
    13861 tgcgatctgg tttccaccgg tgccacgctg gaagctaacg gcctgcgcga agtcgaagtt
    13921 atctaccgct cgaaagcctg cctgatccag cgcgatggcg aaatggaaga atctaaacag
    13981 caactgatcg acaaactgct gacccgtatt cagggtgtga tccaggcgcg cgaatcaaaa
    14041 tacatcatga tgcacgcacc gaccgaacgt ctggatgaag tcatcgccct gctgccaggt
    14101 gccgaacgcc caactattct accgctggcg ggtgatcaac agcgcgtagc gatgcacatg
    14161 gtcagtagcg aaaccctgtt ctgggaaacg atggaaaaac tgaaagcgct gggtgccagt
    14221 tcaatcctgg tcctgccgat tgagaagatg atggagtgat cgccatgagc tttaacacaa
    14281 tcattgactg gaatagctgt actgcggagc aacaacgcca gctgttaatg cgcccggcga
    14341 tctccgcttc tgaaagcatt acccgcactg ttaacgatat tctcgataac gtgaaagcac
    14401 gcggcgatga ggccctgcgg gaatacagcg cgaagtttga taaaaccacg gttaccgcac
    14461 tgaaggtgtc tgctgaggaa attgccgccg ctagcgaacg cctgagcgac gagctaaaac
    14521 aggcgatggc ggtggcagtg aagaatattg aaaccttcca cactgcgcaa aaactgccgc
    14581 cggtagatgt agaaacgcag ccaggcgtgc gttgccagca ggtcacgcgc ccggtcgctt
    14641 cagttgggtt gtatattcct ggcggctccg ccccgctctt ctcaacggta ttaatgctgg
    14701 cgactccggc gcgtattgcg ggctgtaaaa aagtggtgct gtgctcaccg ccgccgattg
    14761 ccgatgagat cctttatgcg gcgcagctgt gcggtgtgca ggacgtgttt aacgtcggcg
    14821 gcgcacaggc cattgccgca ctggcgtttg gtacggaatc tgtgccgaaa gtggacaaaa
    14881 tcttcgggcc gggcaacgcc tttgtcaccg aagcgaaacg tcaggtgagc cagcgtctgg
    14941 acggtgcggc gatcgatatg cccgcaggcc cgtcggaagt gttggtcatt gccgacagtg
    15001 gcgcaacgcc ggatttcgtg gcttctgatt tgctctctca ggctgaacac ggcccggact
    15061 cacaggtgat tttactgacg cccgatgctg atatggcgca tcaagttgcc gaggccgtcg
    15121 aacgccaact ggcagaactg ccgcgtgccg aaaccgcccg ccaggcactg agcgccagcc
    15181 gcctgatcgt gaccaacgat ttagcgcagt gcgtagagat ctccaaccag tacggcccgg
    15241 agcacctgat cattcagacc cgcaacgccc gcgaactggt cgatggcatt accagtgccg
    15301 gttcggtatt tcttggtgac tggtcaccgg aatcggcagg tgattacgcc tccggcacca
    15361 accacgttct gccgacttac ggttacaccg ccacctgttc cagcctcggg ctggcagatt
    15421 tccagaagcg gatgaccgtg caggaactgt cgaaagaagg cttttctgcg ctggcttcaa
    15481 ccatagaaac actggccgcc gccgagcgcc tgaccgccca taaaaatgcc gttactttgc
    15541 gtgttaacgc ccttaaggag caagcatgag caccgtgact attaccgatt tagcgcgtga
    15601 aaacgtccgc aacttgacgc cgtatcagtc agcgcgtcgt ctgggcggta acggcgatgt
    15661 ctggctgaac gccaacgaat accccactgc cgtggagttt cagcttactc agcaaacgct
    15721 caaccgctac ccggaatgcc agccgaaagc ggtgattgaa aattacgcgc aatatgcagg
    15781 cgtaaaaccg gagcaggtgc tggtcagccg tggcgcggac gaaggtattg aactgctgat
    15841 tcgcgctttt tgcgaaccgg gtaaagacgc catcctctac tgcccgccaa cgtacggcat
    15901 gtacagcgtc agcgccgaaa cgattggcgt cgagtgccgc acagtgccga cgctggacaa
    15961 ctggcaactg gacttacagg gcatttccga caagctggac ggcgtaaaag tggtctatgt
    16021 ttgcagcccc aacaacccga ccgggcaact gatcaacccg caggattttc gcaccctgct
    16081 ggagttaacg cgcggtaagg cgattgtggt tgccgatgaa gcctatatag agttttgtcc
    16141 acaggcatcg ctggctggct ggctggcgga atatccgcac ctggctattt tgcgcacact
    16201 gtcgaaagct tttgctctgg cggggcttcg ttgcggattt acgctggcaa acgaagaagt
    16261 catcaacctg ctgatgaaag tgatcgcccc ctacccgctc tcgacgccgg ttgccgacat
    16321 tgcggcccag gcgttaagcc cgcagggaat tatcgccatg cgtgagcggg tggcgcaaat
    16381 tattgcagaa cgcgaatacc tgattgccgc actgaaagag atcccctgcg tggagcaggt
    16441 tttcgactct gaaaccaact acattctggc gcgctttaaa gcctccagcg cagtgtttaa
    16501 atctttgtgg gatcagggca ttatcttacg tgatcagaat aaacaaccct ctttaagtgg
    16561 ctgcctgcga attaccgtcg gaacccgtga agaaagccag cgcgtcattg acgccttacg
    16621 tgcggagcaa gtttgatgag tcagaagtat ctttttatcg atcgcgatgg aaccctgatt
    16681 agcgaaccgc cgagtgattt tcaggtggac cgttttgaca aactcgcctt tgaaccaggc
    16741 gtgatcccgc agctgctgaa gctgcaaaaa gcgggctaca aactggtgat gatcactaat
    16801 caggatggtc tgggaacaca aagtttcccg caggcggatt tcgatggccc gcacaacctg
    16861 atgatgcaga tcttcacctc gcaaggcgtg cagtttgatg aagtgctgat ttgtccgcac
    16921 ctgcccgccg atgagtgcga ctgccgtaag ccgaaagtaa aactggtaga gcgttatctg
    16981 gctgagcaag cgatggaccg tgccaacagt tatgtgattg gcgatcgcgc gaccgacatt
    17041 caactggcgg aaaacatggg tattaatggt ttacgctacg accgcgaaat cctgaactgg
    17101 ccgatgattg gcgagcaact cactaaacga gaccgttacg cccatgtagt gcgcaacacc
    17161 aaagagacgc aaattgacgt ccaggtgtgg ctggatcgcg aaggtggcag caagattaac
    17221 accggcgttg gcttctttga tcatatgctg gatcagatcg ccacccacgg cggtttccgc
    17281 atggaaatca acgtcaaagg cgacctctat atcgacgatc accacaccgt cgaagatacc
    17341 ggcctggcgc tgggcgaagc gttaaaaatt gctctcggtg ataaacgcgg tatttgccgc
    17401 tttggttttg tgctgccgat ggacgaatgc cttgcccgct gcgcgctgga tatctctggt
    17461 cgcccgcacc tggaatataa agccgaattt acctaccagc gcgtgggcga tctcagcacc
    17521 gagatgatcg agcacttctt ccgttcgctc tcttacacca tgggcgtgac gctccacctg
    17581 aaaaccaaag gtaaaaacga tcaccaccgt gtagagagcc tgttcaaagc ctttggtcgc
    17641 accctgcgcc aggccatccg cgtggaaggc gataccctgc cctcgtcgaa aggagtgctg
    17701 taatgaacgt ggtgatcctt gatactggct gcgccaacct gaactcggtg aagtctgcca
    17761 ttgcgcgtca cggttatgaa cccaaagtca gccgtgaccc ggacgtggtg ttgctggccg
    17821 ataaactgtt tttacccggc gttggcactg cgcaagcagc gatggatcag gtgcgtgagc
    17881 gcgagctgtt tgatctcatc aaagcctgta cccaaccggt gctgggcatc tgcttaggga
    17941 tgcaactgct tgggcggcgt agcgaagaga gcaacggcgt cgatttgctg ggcatcatcg
    18001 acgaagacgt gccgaaaatg accgactttg gtctgccgct gccgcatatg ggctggaacc
    18061 gcgtttaccc gcaggcaggc aaccgcctgt ttcaggggat tgaagacggc gcgtattttt
    18121 actttgttca cagctacgca atgccagtca atccgtggac catcgcccag tgtaattacg
    18181 gcgaaccgtt caccgcggcg gtacaaaaag ataacttcta cggcgtgcag ttccacccag
    18241 agcgttctgg tgctgctggc gctaagttgc tgaaaaactt cctggagatg taatgattat
    18301 tccggcatta gatttaatcg acggcaccgt ggtgcgtctc catcagggcg attacggcaa
    18361 acagcgcgat tacggtaacg acccgctgcc gcgattgcag gattacgccg cgcagggtgc
    18421 cgaagtgctg cacctggtgg atctgaccgg ggcaaaagat ccggcaaaac gtcaaatccc
    18481 gctgattaaa aacctggtcg cgggcattaa cgttccggta caggttggcg gcggcgtgcg
    18541 tagcgaagat gacgtggcgg cgttactgga agcgggcgtt gcgcgcgtgg tggtcggctc
    18601 caccgcggtg aattcaccag aaagggtgaa aggctggttt gaacgtttcg gtgccgatgc
    18661 cttagtgctg gcgctggatg tccgtattga cgagcaaggc aacaagctgg tggcagtcag
    18721 cggctggcaa gagaactcgg gcgtttcgct ggaacaactg gtggaaacct atctgcccgt
    18781 cggcctgaaa catgtgctgt gtaccgatat ctcgcgcgac ggcacgctgg caggttctaa
    18841 cgtctcttta tatgaagaag tgtgcgccag atacccgcag gtggcgtttc agtcctccgg
    18901 cggtattggc gacattaacg atgttgctgc cctacgtggt actggcgtgc gcggcgtaat
    18961 agttggtcgg gcattgctgg aaggtaaatt caccgtgaag gaggccatcg catgctggca
    19021 aaacgcataa ttccatgtct cgacgttcgt gatggtcagg tggtgaaagg cgtacagttt
    19081 cgcaaccatg aaatcattgg cgatatcgtg ccgctggcaa aacgctacgc cgaagaaggt
    19141 gcagacgaac tggtgttcta cgatatcacc gcgtccagcg atggacgtgt agtagataaa
    19201 agctgggtat ctcgcgtggc agaagtgatc gacattccgt tctgtgtggc gggtgggatt
    19261 aagtcgctgg atgacgccgc gaaaattctt tcctttggcg cggataaaat ctccatcaac
    19321 tcccctgcgc tggcagaccc gacattaatt actcgcctgg cggatcgctt tggcgtgcag
    19381 tgtattgtgg tcggtattga tacctggtac gacgctgaaa ccggtaaata tcatgtgaat
    19441 caatataccg gcgatgaaag ccgcacccgc gtcactcagt gggaaacgct cgactgggta
    19501 caggaagtgc aaaaacgcgg tgccggagaa atcgtcctca acatgatgaa tcaggacggc
    19561 gtgcgtaacg gttacgacct cgaacaactg aaaaaagtgc gtgaagtttg ccacgtcccg
    19621 ctgattgcct ccggtggcgc aggcaccatg gaacacttcc ttgaagcctt ccgcgatgcc
    19681 gacgttgacg gcgcgctggc agcttccgta ttccacaaac aaataatcaa tattggtgaa
    19741 ttaaaagcgt acctggcaac acagggcgtg gagatcagga tatgttaaca gaacaacaac
    19801 gtcgcgaact ggactgggaa aaaaccgacg gactgatgcc ggtgattgtg caacacgcgg
    19861 tatccggcga agtgttaatg ctgggctata tgaacccgga agccttagac aaaaccatcg
    19921 aaagcggcaa agtcaccttc ttctcgcgca ctaaacagcg actgtggacc aaaggcgaaa
    19981 cgtcgggcaa tttcctcaat gtggtgagta ttgccccgga ctgcgacaac gacacgttac
    20041 tggtgctggc gaatcccatc gggccaacct gccacaaagg caccagcagc tgcttcggcg
    20101 acaccgctca ccagtggctg ttcctgtatc aactggaaca attgctcgcc gaacgtaaat
    20161 ctgccgaccc ggaaacctct tacacagcca aactgtatgc cagcggcact aaacgcattg
    20221 cgcagaaagt gggcgaagaa ggggtggaaa ccgcactggc agcgacggta catgaccgct
    20281 ttgagctgac caacgaggca tctgatttga tgtatcacct gctggtattg ttgcaggatc
    20341 aggatctgga tttaacgacg gtaattgaga acctgcgtaa acggcatcag tgagttgcgg
    20401 ggtaagcgga tgcgatattg ttgtcgcatc cggcaaaaaa aaacgggcaa ggtgtcacca
    20461 ccctgcccgt tttctttaaa gctacaaaga ttacttcgcg ttgtaattac gcagagcgtt
    20521 acgccccaac acaatccccg cgccaaccat gccgcccagc agcaccgcca gaatcaaggt
    20581 aattgccttt ttcgggctat cgcgacgaat aggtaacgtc ggtttcatca catagcggta
    20641 agcatgaata tctagatcat caacccttaa gttttcaata tccaacaggt tttgacgtgt
    20701 ctgatagtag tttggtgaga acaccaacgg acgagtcgct tcatgcttaa tcatcgactc
    20761 cagcgcttcg ctccctagaa ggaacaacgt atcttgcgtc acatcttcag tctgctgaac
    20821 ctgtggcttt gtcacctgcg cctgattcgc atactgcaac gcttcctgaa tctgacggat
    20881 acgcagatct ttctgctcct gcgcgactac ttcctgggtt cttaaagagt cctgcaagtt
    20941 tttccgtccc agagcaatgt tgtctttgag atccttttct agctcttgat tcactttatc
    21001 atcaacttgc tgaatgtatt gggccaactt catttgtgcg ccctctgctg tttgcccaac
    21061 ataagaaaca gtcaatggta attgctggtt cttaacagaa ggttcgatgg taagtttctc
    21121 tggctcttcc tgattatcca gcgtttctgc taatgcagag aaggcagaac tgaagcgacc
    21181 aattaacgtc tcctgcaaat ccgatacttt cggtgcagcc tgaccataga taacattcat
    21241 ggcattgtta tagccagcaa tttgccccac atcgggctga gtgataattg ctgttgacgt
    21301 ccatttctcc ttcgctactg ccaaatatcc aatagccagg gcaatagcca caatgacgga
    21361 aatgatgatt gtcatcttgc cacgccacaa ctgcactagt aaatcaatca aatcaatctg
    21421 ttccgggtca tggttttgcc cagaaacatt attattttct actctcatca ttaccctaac
    21481 tgacggaaat ttccaagtcc gcatagttta gcgataatcc tcgtgaatgc tataggataa
    21541 gtgataaaaa taatgaattg ttgcgtaagt aacaaaagac aatcagggcg taaatagccc
    21601 tgataacaag atgttagtcg ctgccaaaga gatcgcgagt gtatacctta tctgccacat
    21661 ccctaagctc ttctgccata cggttggaga taatgacgtc ggcttgttgt ttgaaggtgg
    21721 cgagatcacg ttccaggcga gagttgaaga atgagtcttc tttcatcact ggctcgtaga
    21781 tgattacttc aacacctttc gccttgatac gcttcataat cccctgaata gaagacgcac
    21841 ggaagttatc tgaaccgctc ttcataatca ggcgataaat acctaccaca tgcggtttgc
    21901 gtgacaaaat ggcatcggca ataaaatctt tacgcgtgcg gtttgcatcg acaattgccg
    21961 agatcaggtt attcggcaca gactgatagt tcgccagtaa ctgcttggta tctttcggca
    22021 aacaataacc accataacca aacgacggat tgttgtaatg gttgccaata cgtgggtcga
    22081 gacaaacgcc ttcgattatt tgacgggaat tcagacctaa actttctgca tagctatcca
    22141 gctcattaaa gtacgctacg cgcatcgcca gataggtatt agcaaaaagt ttaatcgctt
    22201 ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta
    22261 acaacgcagc aaaacgttct gcgcgttctg aacgctcacc aatgacaata cgtgaaggat
    22321 ggagattatc gtaaagggct ttaccttcgc ggagaaattc cggagagaaa ataatatttt
    22381 cagtacgata tttcttatgc atcgctacgg taaagccaac gggtaccgtt gatttgatga
    22441 ccataaccgc ataaggattt atctctacta cgtccttaat tactgattct acactcgatg
    22501 tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag
    22561 catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca
    22621 aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc
    22681 gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg
    22741 acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac
    22801 ttaactgtga atcatgatgt ttttagcatc ctgataagag ctaaaagttt taacgctacg
    22861 ttgttcgatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgata
    22921 tttattaagt taatattaag cagtgaaatt tagtctataa gataattggc aaaaaaaagc
    22981 ccggcgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    23041 ttcggtatgg aacacaccgt ctttatcaat gcgcttataa gtatgcgcac cgaaatagtc
    23101 acgctgtgcc tggatcaggt tcgcaggcag aacagcggca cggtagctgt cataataggc
    23161 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcatatg cgacgacatc
    23221 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggagcca gcagcaggtt
    23281 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    23341 gatgcaacca gcacggaaaa tcttagcgat ttcgccgtaa ttcagatccc agttgtactc
    23401 ttcagacgcc gcacgcagtt gagagaaacc ctgagcgtaa gaaacgattt tacccagata
    23461 cagcgcacga cgaactttct cgatgaactc acctttgtcg ccagctggct gcgcttgcgg
    23521 gccatagaga actttagatg cggcaacacg ctgctctttc agagaagaga tataacgtgc
    23581 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctagt
    23641 ccatttaccg gtacctttgt tagccgcttc atccaggatc atatcaacca ggtagttacc
    23701 gtcttcatct tttttagtga agatgtcttt ggtgatgtcg atcaggtagc tgctcagttc
    23761 accgttattc cactcggtaa aggtctgtgc cagttcttcg ttagaaagat tcaggccgcc
    23821 tttcaggaga gaataggctt cagcaatcag ttgcatatca ccgtattcaa taccgttgtg
    23881 aaccatcttc acatagtgac ctgcgccatc ggcaccaata taggtaacgc atggctcacc
    23941 gtcttcagct actgcggcga ttttggtcag gatcggtgca accagttcat aggcttcttt
    24001 ctgtccgcca ggcataatgg aaggaccttt cagcgcacct tcttcaccgc cggaaacacc
    24061 ggtaccgatg aagttaaagc cttctgcaga aagctcacgg ttacgacgaa tggtgtcctg
    24121 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgaggtatg gcttaaggga
    24181 atcaatagca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    24241 ttccagagat tcaacaaact ctttcaccgt atagtaagga accagtttct tgcctggatt
    24301 ttcagcaatc acttcttcag tcttctcacg ggaacggttg aaaatagaga cggtataacc
    24361 acggctttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    24421 ctgttgcttt gacattgttt actcctgtca gggaaaattt cttatagtaa atgttatatt
    24481 ataaaagact attctatttt atcaaaaaga ataactagat aggtaataat taatcatctg
    24541 aatatccatt ggggttttgc acttgccatt tccaagcatc tcgaatcata tcagctaaat
    24601 cttttttagc tctccaattt aattcttcat aagcttttga aggatctgac caacattcag
    24661 cgatgtcacc gggacgccta ccaacaaata tatgtggtac agcgacattt gttatttgtt
    24721 caaaagtttt tattaagtca agaaccgaat aaccttttcc agtacctaaa ttatagatct
    24781 tacaattctc cccttcattc gatgccttta ccgctgcaac atgtccttcg gcaagatcca
    24841 taacatgaat ataatcacgc acaccagtac catcatgggt attataatca ttaccgaaaa
    24901 tatgaacctc attgagttta cctattgcgg tttggcaaat atatggcata agattattag
    24961 gttttcctct tggatcttcc cctaataatc ctgatgtgtg agccccaaca ggattaaaat
    25021 aacgcaaaat agtacaccga aatgaactat tcgaataaca tatatccgat agaatttttt
    25081 ctattattag tttagatgta ccatatggat tagttgtatg tccaacttca tttgactctt
    25141 ttataggaat actctttggt gatccatata ctgtagcaga tgaactaaaa ataaatctca
    25201 ttacatcatg tcgtaacatc tcttcaagca atgctagagt tccgacgaca ttattatcat
    25261 aataacttat aggtgaacta atcgattgat ctactgattt taaaccggca aaatgtataa
    25321 cagcatcaat attataacta ttaaagattt gattaagtac tgttctattt ctaatgtcac
    25381 cttctataaa tgatgccttt cttaagcaaa tattgttaat cctttttaat actgataaat
    25441 gcgagttaga taaattatca attatgataa cttcgtagcc agcatctaat aacgacacga
    25501 cagtatgaga acctatatac cctaatccac cagttacaag caatttcatt ttatcttgac
    25561 ctcacttaga ctttaatagt tctcacgtta gactcgtttt aacaaggcat gaaaaaaata
    25621 ataaatgata tatttaattg aatgtaagaa tgaaaggcga cggttttttc tataaatatt
    25681 ccactgccag gctattgcct taattttatt tgaggataaa gaatgttcat ttacacgata
    25741 tttagctaaa cactcttgta tacaataagc tctattggtc actgatatta tatccaacca
    25801 cattatataa tcttcatgtc cttcatttac ttggttaaat atacccagtt tttccctatt
    25861 atatatacct gtcaggttac caataaaatt acttttaagc atcatatcat aagttattat
    25921 ttctggtgaa attcgagcat taatatattc tttttcattt gaaaatgtta taaaattact
    25981 gcataccaaa tcccaaccgt cttctaattt ctccatctgt aaagaaagtt tgtttttaat
    26041 ccacatatca tcactatcta taaaggcaac ataattgcct ttacatatat taagtgcaat
    26101 atttcttgat tccgcgacgc ctttgttatt tctgttttta attactgtta ttctttcatc
    26161 ttgaaatttg ccagctaatt cgaatgtatt atcatgcgat gcatcatcga taatatatag
    26221 atgccaatcc ttaaagtctt gatcaattac agagtttaca gcttgttcta ttgttgcaga
    26281 cgcattataa gcaggcatta ttatcgaaac agtttctctt accatatttt atattccaag
    26341 tagtgatatc catttatgaa taattgcatc agcattgtat ttccttgaat ttttaatagc
    26401 attagtacta aaaatgtttg ataaattatc atcagaagtc aattttctta acttctgtat
    26461 aaaaagctct ctgtttttaa aagggataag aaaaccatta acattatcag taataatttc
    26521 tgccgggcca gttggacaat catatgcaac actcgggatt ccaaaactct gggactcaat
    26581 caacaccata ggtaatcctt catataacga agtcatcgca tatattttgg cagcattata
    26641 gtaattgcaa atttcatctg ttgttttaaa cattttgata gatgacattc cagctatggc
    26701 tttttcaaga atccttttat tagggccatc accaatgata tataacgtcc aattaggtat
    26761 atttgcctca tgccaaattt caatcaactc attaaaattc ttttgctctg taaatcgtcc
    26821 aacagcgatc acaatatttt cccgttggtc atatggtttt attatgttat tggatgtatc
    26881 aaatggatta atatttggta tttgaataac gtttttaata ccatattttg tagttaataa
    26941 attataatca tgcgaagtta aaacaattac tttttttgct atgtaatatg aaaatatttt
    27001 tattagctgt attgcttttt taaaactatt taagcctaca tgctcatgga aatatatatc
    27061 atgcagcctg atagctttcg ctaatattcc acactccagc gataagcgtc ccatcgactc
    27121 aacaataact ttatgtttat tcttcttaca aaaaatcaaa taggacaata gagaataata
    27181 gcgccatcca tttccatatt taacagagat tttatttgat aagaaataga aaggattggc
    27241 atgttttttt tgtaatgaga aaatttcgac ctcagcattt tctatattac ttgataatat
    27301 atttgataat aatgctgtaa ctctttctgt accgccctta ttagtaatat cagaaataat
    27361 gaaactaatt ttatttttca ttttttgttt ctcataatat tacgaactat tagtcttcta
    27421 tacagatatt gcatattata aattgataac aatactgtaa gaaatataaa gaaaaatctt
    27481 ctatacattg atttttgttg cactgctaaa gctaaacaac gactagaggt agaatggagt
    27541 ttctcgataa gaatagagga acttagcttt ttatgcatac tcaaaacatc aaaattatct
    27601 ctccctcttc taattctaag tttatggagt tttaacacgt aattatgcgc ttttctttga
    27661 agtaaactag caacacttgc actattgccc tgtcgatgtc taatgtacat tagatactca
    27721 gggtgattat cacatttata accccgatcc aaagctctta atataaaatc ataatcttca
    27781 gcataaggta tttccctata accttgtaac tcatcatata cctccctctt agctaaccaa
    27841 atgtgagcaa ttggagagac atatttagac acctttttaa ctatatcttc tccaacaggt
    27901 gagatacttt ttgtcaaatg ctcacctatc tcattaattg agtgcatttg acatccaaca
    27961 atatctaaat cattcgcctc gagataatgc aattgttttt caattctatc aggtgctgcg
    28021 atatcatccc cgtcaaatcg cgcaatatat tgccctttag ctaacaataa cccctgattt
    28081 aacgatttta caattttctg attttcctta ttatatatta ttttaatcct actatctg
//
LOCUS       DAPOZO010000064        27812 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPOZO010000064 DAPOZO010000000
VERSION     DAPOZO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.064"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(294..1693)
                     /gene="ccp"
                     /locus_tag="ROI21_003007"
                     /pseudo
     CDS             complement(294..1693)
                     /gene="ccp"
                     /locus_tag="ROI21_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
     gene            2098..3747
                     /locus_tag="ROI21_003008"
     CDS             2098..3747
                     /locus_tag="ROI21_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDX5111905.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKACARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3798..4400)
                     /locus_tag="ROI21_003009"
     CDS             complement(3798..4400)
                     /locus_tag="ROI21_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDX5111906.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGAGQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4921..5820
                     /locus_tag="ROI21_003010"
     CDS             4921..5820
                     /locus_tag="ROI21_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5111907.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFIYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPENGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5869..6882
                     /gene="yhjD"
                     /locus_tag="ROI21_003011"
     CDS             5869..6882
                     /gene="yhjD"
                     /locus_tag="ROI21_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDX5111908.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQR"
     gene            7274..8596
                     /locus_tag="ROI21_003012"
     CDS             7274..8596
                     /locus_tag="ROI21_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDX5111909.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8769..10829)
                     /locus_tag="ROI21_003013"
     CDS             complement(8769..10829)
                     /locus_tag="ROI21_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDX5111910.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLASDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10899..11666)
                     /gene="pdeH"
                     /locus_tag="ROI21_003014"
     CDS             complement(10899..11666)
                     /gene="pdeH"
                     /locus_tag="ROI21_003014"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDX5111911.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQSSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11898..12827
                     /gene="kdgK"
                     /locus_tag="ROI21_003015"
     CDS             11898..12827
                     /gene="kdgK"
                     /locus_tag="ROI21_003015"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDX5111912.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12923..14419)
                     /locus_tag="ROI21_003016"
     CDS             complement(12923..14419)
                     /locus_tag="ROI21_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDX5111913.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSANNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGELEFNMKALQAAWDQIMTPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14639..15925)
                     /gene="dctA"
                     /locus_tag="ROI21_003017"
     CDS             complement(14639..15925)
                     /gene="dctA"
                     /locus_tag="ROI21_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDX5111914.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(16108..18096)
                     /gene="hmsP"
                     /locus_tag="ROI21_003018"
     CDS             complement(16108..18096)
                     /gene="hmsP"
                     /locus_tag="ROI21_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDX5111915.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18178..21261)
                     /gene="bcsC"
                     /locus_tag="ROI21_003019"
     CDS             complement(18178..21261)
                     /gene="bcsC"
                     /locus_tag="ROI21_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDX5111916.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAQAQLGDTAAAQQTFNK
                     LIPQAKSQPPSMESAMVLRDGAKFEALAGDPTQALETYKDAMVASGVTTTRPQDNDTF
                     TRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGTGGYSDLKAHTTMLQ
                     VDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDCSGNRSQSDSGASVA
                     VGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAHRRPISSSLLAFGGQ
                     KDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTGKNVEDNWRVRWMTG
                     YYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEYLSFAIPVMWRERTE
                     NWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDGGSSQGFGYTARALL
                     ERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            complement(21243..22349)
                     /gene="bcsZ"
                     /locus_tag="ROI21_003020"
     CDS             complement(21243..22349)
                     /gene="bcsZ"
                     /locus_tag="ROI21_003020"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDX5111917.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPVWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVASGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22356..24695)
                     /gene="bcsB"
                     /locus_tag="ROI21_003021"
     CDS             complement(22356..24695)
                     /gene="bcsB"
                     /locus_tag="ROI21_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDX5111918.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24706..27324)
                     /gene="bcsA"
                     /locus_tag="ROI21_003022"
     CDS             complement(24706..27324)
                     /gene="bcsA"
                     /locus_tag="ROI21_003022"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDX5111919.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWLILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27321..27812)
                     /locus_tag="ROI21_003023"
                     /pseudo
     CDS             complement(27321..27812)
                     /locus_tag="ROI21_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
BASE COUNT         6576 a         7687 c         7004 g         6545 t
ORIGIN      
        1 tccgcgatag tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa
       61 tccgttaaca gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa
      121 gtaggattta tccgcaatgg aagcaaggca ttacaggctt aatttaaata acaaaatcct
      181 aagcagaaaa acgacttttt ataatataaa catttaacat gataatatta aaaacagact
      241 ttattatcgc aataaaaatc acccgaacat tcctgttcgg gcgattaaat taattattgt
      301 ttatcctgca tatacggcgt ataaaccccg ttcagactgt gcaggaaagc tacgatatca
      361 tccacatcct cctgcggcag ctctttgcct acctgatagc gcaacatcag tttcaccgcc
      421 ccgtccagcg tcggcacatc accgcgatgg aagtacggtg ccgttagtgc aacgttacgt
      481 aaaccgggta ctttctgacg caatttatcg cgctcttctt tagtcacatt catacgacca
      541 atatccgccg ccgtaatttc accaaagtta aagtcttttt tcagccccaa cggttcgaag
      601 gaacgtccgc cgagaataat accaccatga caagttgcac atttattatc tttaaataat
      661 tgatagcctt ttttctgttg tgccgtcagc gcattctcat ctccacgcaa ccatttatca
      721 aatggggaat ctggcgtaat taatgttttc tcaaattcag caatggcatc agtaatattt
      781 tcgccactga aaccttgcgg atagacttcg aggaactgcg ctttaagctg cggatctttt
      841 tccagcttag caataatttc gtcccaggat ttcgacgcca tttcaatcgg attcaacggc
      901 ggtccaccag cctgatcctg caatgttgcc gcacgaccat cccagaactg ctcaacgtta
      961 aatactgagt taaataccgt cggcgcgtta atcggcccca ctgcgccacc aacaccaatc
     1021 gatgtttttc tgccatcgac gccccccccg cattcaacgc atggcaatga gcgcatgaaa
     1081 tggtgctatc cgccgataaa cggggatcgt gatacagcgc aaaacccaac gccacttttt
     1141 gcgcatcggt aggcagtttt tgcgggatgg gctgcaccgg ttcattgcga tgctccggag
     1201 caatgtcatt gctggcgtaa tattccgcgc gctgttttgc aatccaggcg agtatttccg
     1261 cccgctcttc atcactcacc ttacccgccc agtgtagcgc ggtataacgt gttggtggca
     1321 tggtttcata ctgcatcacc cattcaatct tattcagatc gctttgcgaa acgggtttat
     1381 cagccagcag agccgcacgc acggcctcga ggttaaaaga tttatatcca agcttaatgt
     1441 cgtaatccat caactgtttc gcgccaggaa tataataata ggcgggtaat tctgccgaag
     1501 gcgtgtggca atagtcgcat cctttttcgc ggagaaagcc taaaacctta ttattttcac
     1561 tgacagcaga tgcctgaaca tcggcctgtt tactgcgttt attatcgtgg taccacacat
     1621 aaccagataa ccctaaatag caaatcgcga cgccagccag gccgatcgcg gtaatacgtg
     1681 agaccatttt cattattctt ccctcacggt ggtgagttat tgtgacccag aaaattttcc
     1741 agaatgctgc aaacgtctct gcgagacaaa gccatcataa aaaacaggga gatacgcttt
     1801 ttgataggaa tcaatcaatt acatgaatat aaactatcaa taagatagcc tgtgcctatt
     1861 atccagatca ggaatggcat atcatgattg cggcgagagc aggattgaat gttctgaaaa
     1921 tgaaaaacaa cacgaaatct gtatttttat aaattccatg gatattagtg cgttatattt
     1981 tttgacaggt taataaacgt gatccaccgc acgctttgtc gcccaccagg cggagcgaat
     2041 gactaccctt aaagaaaagc ccgataatta gcgacgaatt tcggaggttg gatccttatg
     2101 ctcaatcaga aaattcaaaa ccctaatcca gatgaactga tgatcgaggt cgatctctgc
     2161 tatgaactgg acccatatga attaaaactg gatgagatga tcgaggcaga accggaaccc
     2221 gagatgattg aagggctgcc cgcctctgat gcgctgacgc ctgccgatcg ctatctcgaa
     2281 ctgttcgagc atgttcagtc ggcgaaaatt ttccccgaca gtaaaacctt tcccgactgc
     2341 gcacctaaaa tggacccgct ggatatttta atccgctacc gtaaagtgcg ccgtcatcgt
     2401 gattttgact tgcgcaagtt tgttgaaaac cacttctggc tgccggaggt ctactccagc
     2461 gagtatgtat cggacccgca aaattccctg aaagagcata tcgaccagct gtggccggtg
     2521 ctaacccgcg aaccgcagga tcacattccg tggtcttctc tgctggcgct gccgcagtca
     2581 tatattgtcc cgggcggccg ttttagcgaa acctactatt gggattccta tttcaccatg
     2641 ctggggctgg cggaaagtgg tcgggaagat ttgctgaaat gcatggccga taacttcgcc
     2701 tggatgatcg aaaactacgg tcacatcccc aacggcaacc gcacctatta tttgagccgc
     2761 tcgcaaccac cggtttttgc gctgatggtg gagttgtttg aagaagatgg tgtacgcggt
     2821 gcacgccgct atctcgacca cctgaaaatg gaatatgcct tctggatgga cggtgcagaa
     2881 tcgttgatcc ctaatcaggc ctatcgccat gttgtgcgga tgccggacgg atcgctgctc
     2941 aaccgttact gggacgatcg cgacacaccg cgtgacgaat cctggcttga ggacgttgaa
     3001 accgcgaaac attctggtcg cccgcccaac gaggtgtacc gcgatttacg cgcgggggcg
     3061 gcctccggtt gggattactc ttcccgctgg ctgcgtgata ctggtcgtct ggcgagcatt
     3121 cgtaccaccc agttcatccc catcgatctg aatgccttcc tgtttaaact ggagagcgcc
     3181 atcgccaaca tctcggcgct gaaaggcgag aaagagacag aagcactgtt ccgccagaag
     3241 gcctgtgccc gtcgcgatgc ggtaaaccgt tacctctggg atgatgaaaa cggtatctac
     3301 cgcgattacg actggcgacg cgaacaactg gcgctgtttt ccgctgccgc cattgtgccg
     3361 ctctatgtcg gtatggcgaa ccatgaacag gccgatcgtc tggcaaacgc cgtgcgcagc
     3421 cggttactga cacctggcgg gattctggca agcgagtacg aaaccggtga acagtgggat
     3481 aaacccaacg gctgggcacc gttacaatgg atggcgattc agggatttaa aatgtatggc
     3541 gatgaccttc tgggtgatga aatcgcgcgc agctggctga agacggtgaa tcagttctat
     3601 ctggaacagc acaaactgat cgagaaatac catattgccg atggtgttcc ccgcgaaggc
     3661 ggcggtggcg agtatccgtt gcaggatggg tttggctgga ctaacggtgt ggtacgccgt
     3721 ttaattggtt tgtacggcga accataatat ttttacagcc agccgctaac ttcctgctgg
     3781 ctgtaaaatt atcctcttca ggaggagata tttaacatca ttgccgcctg ggtgcgattt
     3841 ttcacttcca gacggcgata cagggattcc agatgcgctt ttaccgttcc ggtactgata
     3901 ttcagcgccc tgccgatctc cttatttgat tcgcccgccg ctaacatggt taaaatctcc
     3961 cgctggcggg cgcttaacga tttgagatct ttaatgtcct tttccggcgt cgtccgccag
     4021 tctccaggca gaaacatcat ccccatcgcc gcactattta ccgccaacgc aaatgtctcg
     4081 acggttgaat cacgaggcac aatggccagc acattaaaat ggataacttc ctgtaaccac
     4141 cgtttattgc aatccgtcgc cgtaattaac accttaacct caggaaattg caccacggtt
     4201 ttttgcagca accagtaaca aaactcacca tcctgatcgc catcgagcat cactaaggct
     4261 tcagggtaac tttccagctt ctgccataac tcgtctgcct gaccggcccc ctgaatactc
     4321 actcctggaa tacgctgctg taaactgatt ttcattccat gaataaatat tgactgcctg
     4381 tcaaacatga ctatttgcat aactgaatct ccacctgaat acgttaaaaa gacttaagta
     4441 gtggaagggt attacccgcg agaaaaaata agaattcgcc atttggcggt ggccattcta
     4501 cagagatgac gcgtagaaaa tagttgccga tataaatcgt tacagctaaa cgcctgaaat
     4561 tacatgtcga gggcactatt taaaacaatt ttgaggattt ccttatattg atggttagta
     4621 cgcatgcaat taaaaatgaa actccgcgac cacaagcaaa aataacaaat ggcaatgaga
     4681 caaaaataag cacaaatagc caacacgtcc tctgttcact ttaaagggaa tcgctgaaaa
     4741 aaaacgctct gtttagggaa attcaccttt ctcataaagc tattccgccc ttttcctgct
     4801 gagaaatcgc cacattcgtc atgacaacat tgtgaaaccc ggcattagat gttagaaaaa
     4861 acaataaaca atgcgatatg cgcgttactg tttcgctgtg aacaacaatc ggtcaaagaa
     4921 atggataaaa ttcacgcaat gcagttgttc atcaaagtcg cggagctgga aagtttttcc
     4981 cgcgcggcgg atttctttgc tttgccaaag ggaagtgttt cgcgccagat acaggcactg
     5041 gaacatcaac ttggcaccca gcttctccag cgcactacgc gacgggtcaa actcacgcca
     5101 gaaggcatga cctattatca acgggcaaaa gatgtgttga gtaatctcag cgaactggac
     5161 ggtctgtttc aacaggatgc caccagtatc agcggtaaat tacgcatcga catcccgcca
     5221 ggaatcgcga aaagcctgtt actgccgcgc ctgtcggaat ttatctatct gcatccggga
     5281 attgagctgg agctgagtag ccatgaccgt ccggtagata ttcttcacga tggttttgat
     5341 tgcgtgatac gcactggcgc gttaccggaa aatggcgtta tcgcccgtcc cctcggcaaa
     5401 ctgaccatgg tcaactgtgc cagtccgcac tatctgacgc gctttggtta tcctcaaagc
     5461 cccgacgatc tgacttcgca cgcaatagtg cgctacacac cgcacctggg tgtacatccg
     5521 ttaggttttg aggttgccag cgttaatggc gtccagtggt ttaagtctgg cggcatgttg
     5581 acggtaaaca gtcgcgaaaa ctatctcgcc gccggtcttg ccggtctggg gattattcag
     5641 atcccgcgca ttgccgtgcg cgaagccctg cgtgccggga ggcttattga agtattacct
     5701 ggctaccgtg ccgagccgct ctccctttcg ctggtttatc cgcagcgtcg ggagctttcc
     5761 cggcgtgtaa acctgtttat gcagtggctg gctggcgtaa tgaaagagca cctggactga
     5821 ccgactatac tttttaagaa tgaccacgac agaaggacaa aagagcggat gacgcaggaa
     5881 aacgagatca aacgtcccac ccaggatctg gagcacgagc cgattaagca gttggataat
     5941 agcgagaaag gcggcaaagt tagccaggcg ctggaaaccg tcaccaccac tgccgaaaaa
     6001 gtccaacgcc aaccggttat tgcgcacctg attcgcgcaa cagagcgctt taacgatcgg
     6061 ctgggtaacc agtttggtgc ggctatcacc tatttctcat ttttgtcgat gatcccgatt
     6121 ttgatggtgt cgtttgccgc agggggcttt gtgctggcct cccatccgat gttgctacag
     6181 gatatcttcg acaaaattct gcaaaacatc agcgatccga cgctagccgc cacgttgaaa
     6241 aacaccatca acaccgccgt tcagcagcgt actaccgtag ggcttgtcgg cctggcggtg
     6301 gcgctttatt ccggcatcaa ctggatgggt aacctgcgtg aagcgattcg cgcccagtca
     6361 cgcgatgtct gggaacgctc gccgcaagat caggagaagt tctgggtaaa atatctgcgt
     6421 gattttattt cactgattgg tttgttgatt gcactgattg tgacgctttc gatcacctcg
     6481 gttgccggtt cggcgcagca aatgattatt agcgccctgc acctgaacag cattgagtgg
     6541 ctgaaaccga cgtggcggtt gattggcctg gcgatttcca tcttcgccaa ctatctgctt
     6601 ttcttctgga tcttctggcg cttgccgcgc caccgtccgc gcaaaaaagc cctgattcgc
     6661 ggaacatttc tggctgcgat tggttttgaa gtgattaaaa tcgtgatgac ctacaccctg
     6721 ccatcgctga tgaaatcccc ctctggcgca gcatttggtt ccgtgctggg actgatggca
     6781 tttttctact tcttcgcccg tttgacgctg ttttgcgcgg cgtggattgc caccgccgaa
     6841 tataaagacg acccgcgaat gccggggaaa acgcagcggt aattcattgg tttataatgc
     6901 ctgataaacg cacggtcgat cccctcgccc cttcggggag agggttaggg tgagggggta
     6961 caagccagcc agagaccagg caatgacatg cacataacca cctgaaacta catctcttca
     7021 ggagcaaaca atttcattcc aactcacaac cccagcatat aaatccagtt ggtaactttt
     7081 atttaacctg aaaccagttt tatccactat ttataaaatt atgtgaagca tttcatagaa
     7141 gaaaaatcac tggcttaaac attatcccct ttttgcctgg cttttgacca tttccgcgat
     7201 ttgttacaca ttgaaatatc acttttgctg tgcgtaatat ggctattcgt tagccaaaaa
     7261 ataagaaaag attatgcaag caacagccac aacactcgac cacgagcaag aatacacgcc
     7321 gatcaactcg cgtaataaag tccttgtcgc ctctctcatt ggcacggcca ttgagttttt
     7381 cgacttttac atttacgcca ctgcggccgt tattgtgttt ccgcatatct tcttcccgca
     7441 gggcgatcct acggcagcaa cgctccagtc gctcgccacc ttcgccatcg ccttcgtcgc
     7501 gcgccctatt ggctctgccg tctttggtca ttttggcgat cgcgttgggc gtaaagcgac
     7561 gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc tgctgccggg
     7621 ctatgccacg attggtattt tcgccccgct gctgctggcg ctggctcgat ttggtcaggg
     7681 tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg gcgactgaaa acgccccacc
     7741 gcgcaaacgt gcactgtatg gctccttccc acagctgggc gcaccgattg gcttcttctt
     7801 tgccaacggc actttcttgc tgctttcctg gctactgacc gacgagcagt ttatgagctg
     7861 gggctggcgg gtgccattta tcttctcggc ggtactggtc attatcggcc tgtatgttcg
     7921 cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct aaagcgaaaa aacaggtgaa
     7981 gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg gtacgttcat
     8041 tatgctggca acctatacgc tgttttacat catgacggtc tactctatga ccttcagtac
     8101 cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga tgctgatgat
     8161 ggcagttatt ggttttggcg tgatggtacc tgtcgccgga ttactggctg atgcctttgg
     8221 tcgccgtaaa agcatggtaa tcatcaccac gctgatcatc ctgttcgcgc tgttcgcctt
     8281 taacccgcta ctcggttctg gcaacccgat tctggttttt gccttcctgc tgctggggtt
     8341 aagtctgatg ggactgacct tcgggccgat gggcgcgctg ttaccagagc tgttcccgac
     8401 agaagtgcgt tacaccggag catcgttctc ttacaacgta gcgtcgattc tcggggcttc
     8461 cgttgcgccg tatatcgcag cctggttgca gactaactac gggttaggtg cggtggggtt
     8521 atatctggcg gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc atgagacacg
     8581 acatcagtcg ttgtaagtat cgtgcgggct gccaccctca ccccggccct ccccccagaa
     8641 agtgaacgga aaagcggtac tcgttgtttc aacacgcaca aaacagtccc ctcgcccctt
     8701 tggggagagg gttagggtga ggggaacacg ctgctcccga actcaccagc aaacacaatc
     8761 caacctcatc acttcttcat ctgcgacaaa atcgtccggc actgattagc ctccccttcg
     8821 gaaggtgaga tcagcgccag taacgccgcc gctggcgtaa ccagcgttgc cagtgccgcc
     8881 gcaacggctc ctcgggcaat caacggcccg gctttcaccc cagcctgcgg atttttaaat
     8941 gtcccacgca catacagcgg cgaacgcagc gtgataatcc gaattccttt actctccgga
     9001 tcaatagtca aatccagctg ttccgaagca aaacttgccg tgccggtaac gttaatcaac
     9061 gcgttctcag tatcgaaagc aaaaatctgc gggcgcgcca cgccgttggc aatatccaga
     9121 ttcgccgccg cacagttcac ccgcacctca tcgtcaccaa atatcgcacc gacaatgtag
     9181 ttaccgacat tcagcccgac aatctccatc aggttgcggc tcaccagccc gtcattcatc
     9241 aacagtttca ggttgccgtt actgttgcct aaaagcgccg ccaccgagtt accgctaccg
     9301 cgtagttccg cgtcaccgtt catttccccc agcgtcttct gcatcagttc cacatcgggc
     9361 atcagttctt tcagtttcag ccgacgagcc tgaatatctg cccgcccttg catcggtttt
     9421 ttatcacctt ccagatgaat attcgccgca atgctgccgc ctgccatgcc aaatttcagc
     9481 ggttgcaggc gcaggtcagc atttttgagg atgatatgag tagaaagatc gctaatcggc
     9541 aggctactgc catgctcaat gcgccgccct ttgaagcgaa catcggcatc catgacgtcc
     9601 catttatcgg tttcgaagcg gtcataaggc agcactttac ccgcaggctg aacgcttttt
     9661 tcgcccttct tctgttcaga ccgtttcgac ttttcagctc ctttcccgga atcaacgcca
     9721 atcaacggtc ccaagtccgc cagccgcaat tgccgcgact cgacatcacc ttccagtttt
     9781 ggccgtggct tgccggtggt atagaccaga gaaccgtgga tatcgctatc gccaatgcgc
     9841 ccattaaagc cgcgataatc aaagaccgac gatttttcag tgtcgatttt cgctaccagc
     9901 cgaccatccg tttcaaacgg cggggtatcg ggcagcagaa cgcccgtcag ttcatagaga
     9961 tcacccagcg aatcgccaga aaatttaagc cgtaaatcga caccacccat cttcattggg
    10021 tcattcacaa cgccatcaaa agcaacacgg gtgttaccag agcggaaatc agcctgtacc
    10081 ggaaacggcg tcccttcgcc acgcagcgcc agcataccgc ctatttttcc cgtaccagtg
    10141 agcggttcac cgttataacg tccctgcgcc ttcaggccaa aaacgtaatc gcccaccttt
    10201 tctttatccg ctttaccttt cgatccagta acttcgctga acggcagcgg cttgcctaac
    10261 ggatcgacaa aaatttccag atccgctttg cttactttgt catcaatggc gatccgccct
    10321 tgatcgaaaa gaatattatc cagccgaaac gaccatgccg acggctttgc attcgcgtct
    10381 ttgttgtcat cgctggcgag attaaatgtc cagttgttgt tcttttctga gaggcgaatc
    10441 aggcgtgcgt cgggcttttc cagtttgatc cacggtagcc agaccgtttt ggtcagcagc
    10501 gccaacggtg ccagcgttgc ctctacgcgt ggcaaatgca ccattgtgac ttcgggaata
    10561 tccggtggat tgccgagaat gatgtcttct gcatgcacat gaggccacgg cacccagctg
    10621 cgccagccag tttcttgctt ttgccgctcc cacaccacgc ccagatcgcc acggatagcg
    10681 aacggacgat tcaactccgc agagactttc tggttgatgg tcggtttgag tcgattccag
    10741 tcaaaagttg caatcaaaat gatcgccacg acaatcaaca acaagaaagc ccctgaaatc
    10801 gcagcggtta ttttgcctgc cttgctcatc gttcgcttcc tcatattctt cctgtgccag
    10861 tcctaaagat agtccagcca ggcggaaaat gaggcagctt atagcgccaa aaccgccgta
    10921 ttcagcgttt ctatcggtgc cgggcgtgaa agaaaccagc cttgtgcggc gaatgcgggc
    10981 gagctctgaa catcacgcca ctcttccggc gtttctacgc cctcgacgat caccccgcga
    11041 caatagcgat tcatgagatg taaaagctga gaaaagagtg tgcgtccttc cggcgactga
    11101 cgcagcatca caaacagctc tcgcgcgatt ttgatgtagt cataacgcac ttcacttagc
    11161 gcagagaaat ttgccatccc ggtgccaaaa tcatccagcc acagcgggcc aaattcacac
    11221 atcgaggcaa aggttgaatc tttcggcaga cggatgtgtt ccaccagttc gaaacgcagc
    11281 cagggaagac gctcaatctg gcgcaggatt tttggttgct gacgcagggc gatgagcgta
    11341 gggccatcaa tattgaccga tgccagcagg tcatgctcta taaagaagtc ggctttttgc
    11401 gccagtaaat caatctgctc tttcacaacc tccatccgat ggctgacggt gatttcagta
    11461 aaatagcgat ccggcggcag gcgttgcgaa gggttcaagg gatgcgtgac caccgttaat
    11521 agctccacgg ccattaaccg cccgcatgtt tgatagatcg gctgccaggt gtaagcacgc
    11581 tcacactgca accaaaaacg ccgttcctgc aagctctcga tgcttgcttc agggttgctt
    11641 attcgctgga taacctgcct tatcatcaaa gatgtcctgt gtttaaaagt gatgctgccc
    11701 ggactcgaca aagattatcg gcgcgcaagg gcataacttt accgcctggc cgggttaaat
    11761 tttcccactc cacaacggaa tctttcagga atacgttctg gctcaaaaaa ataatggaac
    11821 actgttttaa tatggttgac cagcaaacca ccacagcgca aactaacgct aattttttac
    11881 agatcaggtt cacgactatg tccaaaaaga ttgccgtgat tggcgaatgc atgattgagc
    11941 tttccgagaa aggcgcggac gttaagcgcg gtttcggcgg cgataccctg aacacttccg
    12001 tctatatcgc ccgtcaggtc gatcctgcgg cattaaccgt tcattacgta acggcgctgg
    12061 gaacggacag ttttagccag caaatgctgg acgcctggca cggcgagaac gttgatactt
    12121 ccctgaccca acggatggaa aaccgtctgc cgggccttta ctacattgaa accgacagca
    12181 ccggcgaacg tacgttctac tactggcgta acgaagccgc cgccaaattc tggctggaga
    12241 gcgagcagtc tgcggcgatt tgcgaagagc tggcgaattt cgattatctc tacctgagcg
    12301 ggattagcct ggcgatctta agcccgacca gccgcgagaa actgctttct ttgttgcgcg
    12361 aatgccgcgc caacggcgga aaagtaattt tcgacaataa ctatcgtccg cgcctgtggg
    12421 ccagcaaaga agagacacag caggtgtacc agcaaatgct ggaatgcacg gatatcgcct
    12481 tcctgacgct ggacgacgaa gacgcgctgt ggggtcaaca gccggtggaa gacgtcattg
    12541 cgcgcaccca taacgcgggc gtgaaagaag tggtggtgaa acgcggggcg gattcttgcc
    12601 tggtgtccat tgctggcgaa gggttagtgg atgttccggc ggtgaaactg ccgaaagaaa
    12661 aagtgatcga taccaccgca gctggcgact ctttcagtgc cggttatctg gcggtacgtt
    12721 tgacgggcgg cagtgcggaa aacgcggcga aacgcgggca cctgaccgca agtaccgtta
    12781 ttcagtatcg cggcgcgatt atcccgcgtg aggcgatgcc ggcgtaaaag cagcatatgc
    12841 aaaaggctgg ataaagtgtt acccctctat ccagctcatc cataaatacc tgatggttgt
    12901 gctaactcat caccatcagg cattactgtg caggtggaat atccgtcact tcaggatgaa
    12961 cgtcatccgt ggcgacagag gtggtcgcag cggcagtaga tggggtcatg atttgatccc
    13021 aggccgcctg caacgctttc atgttaaatt ccagctcgcc tttcggctgc agcagtatta
    13081 acgccatatc gttcgacaac tgctgacgca gatcctgatt taacatctcc accgtcaggc
    13141 tgttaaggaa gtcctggcgc agtttctgat attgctccgg cgcgatatcg acaacctgat
    13201 tttgcaacga acgcatccgc tgccccatca gaatatcggt atcagctcgc gcataggcgg
    13261 caaacagttt ctgcagctcc agtttctttt gcgccactaa cgcattgaac tcctcttccg
    13321 gcagaccttt atcgcgaacc ttcgccagct cacgcgccac cagattaagg ttgctgttca
    13381 gcttgtcgtt tggtgattcg atgttgatgg cacactgcgc acgcagatac agcacacggc
    13441 agtcaaatcc aagaccgatg tctttgctgt tattggcgct taacgcttgc tgaacatgcc
    13501 agaacagcgc ctcacgggcc aggtccgcac gccagtagcg cagcagtgcg gctgattcac
    13561 gaatcggctg ccacggcgta tcccacatga tggataaccg gtcctgacgc accgcgtcag
    13621 tcataatact caccgcttcc gcacgcagcg gagaaagcgt cggcaccgga gccggtgttt
    13681 cacgtttgcc tttcagttcg ccaaacgttt tattgatttg gtcgacgacc gagcgcgcat
    13741 ccacgtttcc caccaccagc agcgtcattg catccggggt gtaccatttc tgatagaaat
    13801 ctttaatctt ttccgcttca acgggttgtt tcagcggatc ggcaggatcg tgacctaaca
    13861 aggttgatcc tttcagacga tagcgccacc agccctcttt agtatcggca ggccaggttg
    13921 ccaccatgtc ctgactttgc agcgcgtggt tgatggtttc tggcgtgata gtcagtttgc
    13981 cagtggcatt tgccagataa gagagcgctt ctttcagcaa gtcgttacgg ttattgggca
    14041 aactcagatt aaacagcgtg gtgtcataag agacaattac cggcggcatc gggcgtttag
    14101 ggtcgatccc ctgctgccac aatgaacgcg cctgtgctgc ttcaaggcca ccgctttgcg
    14161 ttaacgcaat acgagggatg gcgtggctgt aaccgctctg ttgtgtactt tcggcgagcg
    14221 aaccggtatt aaccagcagg cgaatttcaa cacgatcgct gggacgctgg ggcgtggtca
    14281 gcacttgcca ctgtaaaccg ttggaaagcg tcccctgttg ccatgctgga tcaggctgga
    14341 gcgcatctgc ctgcacatag ccagcagtgg ccatcatcag caaaccgccc gctaaaagtc
    14401 gaatttttgt gccctgcatg tgaacccctg atcaactatc ctggtaataa aaaactgccc
    14461 gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa gaacatgaaa
    14521 atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc aggcacaggg
    14581 gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag tggtgagatt
    14641 aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca tcgtccagtt
    14701 ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg ccgttaccga
    14761 ccaggttagt cagcgcacga gcttctgaca taaagcggtc aataccgagg atcagcgcca
    14821 gacccgctac cggcaaatgg cccaccgcag agagcgttgc cgccagcacg ataaaaccac
    14881 tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac agcgtgattt
    14941 ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc gccattgtca
    15001 ggtatatcga tgtaccatca aggttaaacg agtagcctgt cgggatgacc agccccacca
    15061 ccgatttgcg gcagccgagt ttctccatct tgtcgagcat acgcggcagc gccgactcgg
    15121 aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata aatttgaaga
    15181 tactgaaacc agtcgcttta gcgattgaac ccagtaccag caccacaaac aggatacagg
    15241 tgatgtagaa gcagataatc agctgcccca gttgcaccag tgtgccgacg ccgtatttac
    15301 cgatggtaaa cgccattgcc ccgaacgcac caataggtgc cagacgcatg atcatattga
    15361 tgatgccgaa gatgacctgc gagaaacttt cgatcacgtt aaaaatcagt tggcctttgc
    15421 tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc tgcagaatgt
    15481 taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg aaggcgacaa
    15541 tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc tttcgcatca agcgttgccg
    15601 gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga ccaataatca
    15661 gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta cgaccgaccg
    15721 ccttcatgct ttccatgccc gcaatgcccg ttacgacggt acaaaagatg acaggagcga
    15781 tgatcatctt aatgagctta acgaacccgt cgccaagcgg tttcatttgc tcgcctattt
    15841 cagggtagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc tgaaagtaaa
    15901 ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag gtcttgtaag
    15961 gttatggggt acctgcggcc ttaaaataac acccagacaa catcacagaa atgtacctgg
    16021 atcataattg aaacaaaaag gttaaaaagt ttgagctggc tcgcacaaac cagcactttt
    16081 taaagttttg taatcagttt ggggtagcta cttttcttcc aggtaactct cttcgaagat
    16141 ttcaataggg agtgggcgag caaaaaggaa gccctgggca ataccaacgc ccgcttttgc
    16201 cagccagtcg cgttgtgctt cagtctccac gccttcggca atcatttgta agttcaggct
    16261 ttgcgccaac atgataattg cagcaatcat gctgctatct tccggcaagc cttcaacaaa
    16321 cattttgtcg attttcagta cgtcgattgg caacgatttc atatgctgca gctgacgcag
    16381 ccccgcatag cccatgccga aatcatccag cgccacccga actccggcat tgcgcagcgg
    16441 acggaggatt gccaccgcag catgagggtc gtcaatacgt cggctttctg tcacttccag
    16501 aatcagtgtt cccggctgaa tgcgatagcg ggttaacagt tccagcatat ccgccaccat
    16561 attcgggtgc atcagttgca gcgctgagag gtttaccgac aagggcagca taatgccgcg
    16621 ctcttgccag gcagcaagca gtcgacagga ctcttccagc acccagtgac caacggtaac
    16681 catcagccca cagcattcaa tgcgatcgat taagccatcc ggcagatccc aactgccatc
    16741 cggttgctgg atacgcagta acacttccgc actgaccagt ttaccgctgg tcatctcgac
    16801 ctgtggctgt aaccaaatcg caaactgatg attttccagt gcattaagga tatcgctctc
    16861 ttccgtcaac cgctgttggg cggcttccat ctgctgcgga tcaaagaact gaatctgatt
    16921 cttgccttta tggcgagcgg taaatgccgc agaaatagca cgactgtaaa gctgttcggc
    16981 ggtgagatcg ccgtagaaca tcgccacgcc aatgctacag tgcggacgga gttgaatacg
    17041 ttcaatcggc aggcgctcgc tcatgatagt gagcacttgc tgacctaaag tgattgcgtg
    17101 ccacggttcc tgtacaccat tggcaatgac agcaaagtca taaccactaa tctgcgcgag
    17161 gatcatacgt ggcgacagta ccgatttgag tttttccacc agcgtcagca gcagaatttc
    17221 tcgttgcgcc tctttcagca cgcccgcagt atcacgcagg gtttcacagg tgatgatcat
    17281 cagcgcggtg gtttgtttac gcgcgacaac ctgctccagc atctccatca gcaaggcttt
    17341 gttcggcaaa tccgacaccg ggaagcgcat cgcgttttcg ttctgttctt cataatggcg
    17401 ctgcagcaat tgctggttga ggttgtaact gcgcaccagc ataccgattt catcgtcctg
    17461 atgcagacgt ggtaatgcca gttggtgacc aacaagctcc tgggcaggga tggcgttaag
    17521 ttcgcgagca atattgcgta acggatgcaa aatcaggcgg ttaatgcacc agctaatcgc
    17581 caccgtcaat attaacgaca aaagtaagta aatggtcact aacgttgaga gggtgctcat
    17641 cacgaactta tacatgcgga aggaatccgc ctgtagcacc agataggcaa ttggctgcgg
    17701 atttgccgga cgttcgagcg agtagacacc cagcgagatt tgaaccggta gctcaaacag
    17761 gcgagtaacc attaccggca ctggccgctc tggaataaaa cttttacgca gcgcctggaa
    17821 ctggttaggc agcactacat cggcacggct gaccacgcct gccggtttaa tgctggcaag
    17881 aatagcttcc gcttcgggaa tatcgccttt caaaatagct gaagataagg gttcgcggac
    17941 agagcgagca atgctttcca gttgcgtagc cgtgttatag cgattctgct ggaccagatg
    18001 gaacagcaaa acggtgcaaa aaataaaaac gaacaccagg acaacggctg ccaccattgc
    18061 catctgcttg attgttaacg agcgacttac gcgcaaattg tctccccccg gaaatcccag
    18121 attgccgccc gagtataccc gatcgcagcg gcattaagag aggcgctatc tgaaaactta
    18181 ccagtcggcg taaggtatca gcggctgcgg cggtaaatcc atgtcaccct gccatccggc
    18241 ggcggaataa cgtacgtaga gcaggaaatg gctgggtgcg taatctttcg cctgctggat
    18301 atcaattgcc gtgccgacaa accagttgga agtaacacgt cgttcaagta atgcccgcgc
    18361 cgtgtagcca aagccctgac tgctgccgcc atcgttcgat tgccgcgcag cttcttcctg
    18421 ccagtcggtc gggatcagat tcatcagcgg ataacgcggc atggttttgg tgcgtgaatg
    18481 cgaccacgag ccagacgccc ccagctccca cgaccagttt tccgtccgct cacgccacat
    18541 caccggtatg gcaaacgaca ggtattcctg cgggctgtag taaccgccct gaccgagtga
    18601 gtagccactc aggtctttgt cgtaatgcca gatcatgttg ttcaggccga ttgtgacgcg
    18661 gcgattgttc tggttaatga ccttatagta atagcccgtc atccagcgca cacgccagtt
    18721 atcttcgaca tttttaccgg ttaactggtc accgctaagc gatgcccaga cgccgtttgc
    18781 ttcaccttta tcgtagctca ggcttagccc cacgccgtcg gcacgcacgc caccccattt
    18841 tttcccggta ttgctcgggg agtctttttg cccgccaaag gccagcaaag aactggaaat
    18901 gggccgacga tgggcgttaa cggtgtaacc cagcggcccg atatcatcgc tgtaactgat
    18961 gccgccgacc acatccacca cgttgaagcc catcggcgtg gtaccgatat cccagctcca
    19021 gacgtcattt cgccagccga ccgccacgct ggcaccggaa tccgactggc tgcggttgcc
    19081 gctacagtcc tgtaatgtac aggtgcccca gttgtcatcc catttgccat cggcattcgt
    19141 ggagaaactg ccaacgttca tattgacgaa atcactgcga aagaacatcc gcccgtcaga
    19201 atacggcgca tccacctgca acatggtagt gtgcgctttc agatcggagt aaccaccggt
    19261 gccgctcgaa ccccagtaat cgtgctcgag ggtgacgtta agatcctgct ggcgatagag
    19321 gtcagccgca tcgctgcgca cgccgcgttt cagccagtca tctttctcgt cgttacgggt
    19381 cagccgggta aaggtgtcgt tatcctgcgg acgcgtcgtg gtcacaccgg atgcaaccat
    19441 ggcgtctttg taggtttcca gcgcctgcgt tggatcgccc gccagcgctt caaacttcgc
    19501 accatcacgc agtaccatcg cgctttccat cgacggtggc tgagattttg cctgcgggat
    19561 caacttatta aatgtctgct gcgccgctgc ggtatcgcca agctgcgcct gtgccagcgc
    19621 cgctctgtcc tggtcatgac cagagagata cagcccgtaa gcgtaaacct gctccgggtc
    19681 gttcggcttc tgctgcgcca gattacgcat taacgtatcg gcctggctgc gttgtccggc
    19741 ctgccagaga tcctgcgaaa gtcggtaagt aatccatacg ctgccggggt ccagcgccag
    19801 tcgttgccgc tgaagtgctg ccgcctgcgc ccatttgccc tggttttcca gtgcctctgc
    19861 ctgctgtgcc agacggtcgt tttgcaggct gcgttcgata tcatcaatgc ttcgccgctg
    19921 actggcagag agcgaggcga taaacgcttc agctttttct ggcgattgct ggcggtatat
    19981 attcgccagc ccgcgcacgg cgttagtgtt gccgctgtcc atacgcaagg tctgctgata
    20041 ataacgttcg gcggcgggat aatcttttcg cgccatcgcc acatcgccca gccccagcac
    20101 tgcatagctg tcggtgttat cgacattacg cgcctgctgg aacaggcgtt ctgcccggtc
    20161 aggattattg gctttcagcg cagcatcgcc ctgctggatc gccagccagt agcggtttac
    20221 tttcaggaga ctgttccatt tgtcgttgtt gctgctgtgc ggatccaggg cgagggcttt
    20281 ttccagattc gccaccgcat tggcgcgatc gcctttctga gaatacgcct ggcccagcgc
    20341 ccccagagcc tcactgtctt tcgggttcgc ccgcactgcc tgttgtagtt cgggaatggc
    20401 tttacccgcc ataccagagt ccaccgccgc taaaccttgc gcacgagcgc ggaaagcagg
    20461 gtcggccagc tgtttttgct gttctgccag ttgcgattgc gccgcagcta cgctatcgcc
    20521 atcactaaag atcgagagat attttttcag cgccgacaca ctggcatcgc tgacgggcat
    20581 gtctttaatc tgcccgtacc agattttaga ggccccttcg cgcccggcgt ttgatttcgc
    20641 catctgttcc aggacggcaa aaccttcgtc acggcgatcg ctactaaaca gcaatagcgc
    20701 cagattgttt tgcaggcccg tattgcccgg cgtatccgca ttaatgcgtt ttagctgatt
    20761 aatcgcttcg ccacggcgag ccggaatttt cgccaccgta ctccagtact cgacggcaat
    20821 gtcaccttcc ggcggcgcac cgttgaacag tttgttgtaa ctcgccacag cttcttctgc
    20881 atgaccagta gtcgcctgca atcttgcctg ttgcagtgcc tgacgaccat ccggcgtgga
    20941 aagcaacatt gtggtccgcg acgatttata cgcatttgaa ctcggcgcta actgcgacag
    21001 ccgatcgagc tgtttttgcg cgccatcaat atcgccctga cgtaacagag aacggaaacg
    21061 ggcggcaacg acgtccgggt tattcggatc aataagttcc agacgataca acgactgttg
    21121 caccaggtct tcacgatggg tcgcttcgcc taaccgaact tgctccagca actgttgctg
    21181 agcggttggt gctgcctcga ccatcggcat gacggccaga ccgagggaaa gcgtgaatat
    21241 gtttagtgtg aatttgcgca ttcctggccc cagtcaggta ataactcacc ttttgtcgag
    21301 aagcggaaac ggtgttgatc ccagccttgt ccaaacaggg tcagcacata gttgtaatag
    21361 gcatcgctgc cgggaaagtt atcggccacg cgctggcgct gaacggcctg cgcatcgcgg
    21421 ttttgtaaaa agggcagcat ggcggcagaa aaaccgaccg gtcctttacc ctgcgctttc
    21481 cccgaagcta catcgacttt ttccggcgga taaccgtttt tctcggtgaa tgtcgccatc
    21541 ggtttaaacc ggttgagcat ccgcgctttt tgcggatcgc tgtcaggcat catgcctacc
    21601 cacatgtaaa cgcggatcgc gtcgtagctg ctgatcaatg ttttttcggc ttttagctgc
    21661 cagcctttgt ctttctcata gcgcacccag tctggcgaaa agcctttcgg ggcggtttcc
    21721 agcaataaac gttgattggt ttcgcgcagc gtggtccacg gcgcgccaaa gcgggtgaaa
    21781 tactgcgcca gcgtcggcgg caggtagctg gggttaaaac gccagctgtt atcctctgca
    21841 aatcccactt tgcccggcaa caacatggaa cccagtccag gcaccgtcac cacttcctcc
    21901 cgcgcaatac gttttaacaa cgcgctgccg atgtcggtat aacgctgctc tttccacaaa
    21961 cgccccgcct ccagcaacga ccaggccatc cagacatcac catcggaggc cgaattgctg
    22021 tccagcactt cccacttact gttctctttc ttgccccaca gccatacggg caagcgttct
    22081 tttaaagaac cctgagcgag attgttctgc gtccagtcga gaatattatc gaaagctgca
    22141 cggtcgttag ccgccagggc aaagaacatg ccgtaacttt gtccttcgga ggtggtgatt
    22201 ttgcgcgcgt cgctggggtc gatgacgcgc ccttcctgac tgatgtaatc ctttttaaac
    22261 tgctcccagg caggccaggt acaggctgcc tgaacactaa aggcagccag cagcagcatc
    22321 gtcacgagtc cactacgcaa cacattcatc ttcaattact cgttatccgg gttaagacga
    22381 cgacggctga taatacgcag cagacgccac agtacccagg ccagcagcac gacgctgatt
    22441 gccgccagca ccgccagcag aatcggatgg tttgccaggg cataccataa gcgttcgaac
    22501 cacggcagat ggccaacgta atagacgtcg ccaacacgca ggctattgat accggactcg
    22561 cggatcaccg cgaccgaacc gaacatggtg gcgcgtttgc cgctatcgtt caccgcatcg
    22621 ttaagcattt cataaccgcg cgggctatct gccagcagcg caatcacgct acgctggtcg
    22681 ttatacggcg actggaagcc aatcaccgcc gccatcgcac cggaagaggt cagcgttgat
    22741 tgagtttctg ccgcgcgatc gctctcatcc ggtacaatgc cggggaacgg ggtctggcgc
    22801 atcggcgttt tcacccagct ttcggtcgcc tgcaccaaca ggtcgatctg cttatcgtct
    22861 ttcagtttgt ccgggatacc accgacgatc atgatgtcgg catctttgcc ctgaatggtg
    22921 ctgccatcat cggtcaccgt caggttaatc gccgggaagc ccgtctgcgc accgataaag
    22981 ccaacggtat tcagcaaggt ttccatctgt gcttcgttag gcgctttcgg catcacggtg
    23041 atggtttgtg acagatccgc catccggctg aacgggaagc ccgcgttagc aaaggcgcgt
    23101 agatccggca tcgggatgaa gtggtaatac ttcgagaagt cgatggtgga gtcgtcacca
    23161 atcaccacat gattctgcac cggctggaag gtaatacagt tatccaccga accgcccggc
    23221 atcgggttca tatactcaaa gtcgaaccgc agctggttgg tcgcgcccag tttcagcgcc
    23281 ggaatagaga catccgtttt gccatccagc aaaccttgta ataccggaat acgcagcagc
    23341 aggcggttcg cctcctgttt actgctcagg ttgaaggatt gcaggaactg gttattcagg
    23401 ctgatatcca tccgcgaact gtctttcacc ggcggcatgg tgtagcgata gttgatatcc
    23461 atatcaatgc cggtactgcg catcaggtag agatccggcg gcaggtttag cgaaacgtta
    23521 atcgctgctg gctcaagacc gctggattgt aactgttctt cataggtttt cagttcacca
    23581 aaggtaaccg gacgatcggt acgcacccag ttcggcgcat cgtacggctt acgcggtagc
    23641 agcggtttca cttcattcac taccacgctt tcaccacgga acagaatgtt accctgagcg
    23701 atacctttcg ctgcctgcaa caggtctttg tcgtcacgac caaacaccac cagcagtttg
    23761 acgtaaggat tctgtggatg gttgatcatc tcaatcaccg gggcttttac cgccggatga
    23821 tcgcgcagga agtccgggcg tttgtcattg gtggcaaaaa cgatggcatt gcgatccggc
    23881 agttggttat agagcaccgg gaagttctgc ccacgccagc cagaacgcga accaaaccac
    23941 gaggcaacaa tggcagatgc ttgttgcagc ccgacatccg gcgcacccgc aaagaccatc
    24001 ggcaaggtgt tggtgcggtt atcacgcggg tcaaagaatg gtaccgggaa gtgtgacagg
    24061 tcattcttca cattcagggt ctgataggtc aaatccagtc cactgctccg cccaacatcc
    24121 agccaaagcg tggtgctggc cgggttttcg cacacgtcct gataatggcc gacaaactcc
    24181 agccgcacac ggttgaagtc ggtaataaac agtgggttaa tgggcatttg cgccagcgtt
    24241 tttttaccca actgttcttt ggtcactggc agcacgccca tcagttcatc attgagataa
    24301 acctttaact gcgactggac aggcagtaac gatggcgatg gcgtgtattc gagattgagc
    24361 atcgccttcg ttaccacttc atcgctgcgc ataccaaact caatgctgcc gttcgggtta
    24421 atgccacgta gcaccatgct gcccggcggc ggcgcaattt gcgcaaaggt cagcttcaca
    24481 tcacgcgaag gaccgttctg cgccacgact ggcgcatcag cgccctgcac gcccggcatc
    24541 acttgcccaa cctgcggatt ttgttccgtc tgggcggcta cagctggctc agcattgatc
    24601 agtggttgcg ttgctggcgt cgcctgcgtc atgaaagagg ggaacgcact catccccata
    24661 gccactgcac aaatccagaa tagttttctt ttcatcgcgt tatcatcatt gttgagccaa
    24721 agcctgatcc gatggttgtg ccgtttcgct ccgctccggg cggcgcggaa taaacgatac
    24781 aacccaggaa accagagaag tcagcacacg gaatatgccc ttcaccgaag aaggcgcaaa
    24841 ctccgccaga tggcggtagc cacggaagcc gagcttcaga atatccagca gactttccag
    24901 cggcttatct tccggatagc tgtcctgcca gagcgcccat gtatccgcac gggcaaacgt
    24961 acactgcaca aaatcgatat gttgctgggt ggtgagcggc attaatttca acccaacttc
    25021 attacccatc acgcgcgcca cctgggtcgg gaagacgtat tcctgctgac cacgtttaag
    25081 caacagattc actttctgcc cttccagaat ctgcgcctga ccgttgatct tgatccccaa
    25141 accaccgtcg gagaaatcct gaacggtaca cgagaagagg tgaccatctt cgcgggcaat
    25201 tgccgcgggc atcgtcatct ccacgcggtg cgatcggcgt acctgtttgc tttctaccga
    25261 taccgcaact gcgccgccaa gaacaatcag gttgtagaat acccacacca tactgacgac
    25321 cacggtgagc atctcggttg gcgggccata gaagtagcgc cagatgccta ccgcaacgcc
    25381 caccaggttg agcagaacaa ggaagatgta gggccgcgag atcacccagt cgacgtactc
    25441 ttcttccacc agtccacctt tggcggtgac gttaaatttg cctttgtgcg ggttaatcag
    25501 cgccaccagc gtcggtggtg cgatatacca cgccagcacc gtttcgtaga tttcactcca
    25561 gaaagagtgg cgatatttgc cctggatctt ggagttggtc aggctggcat gaatcatatg
    25621 cggcagcacg aacagggcaa tcatcaacgc tggcgcatag atgatgtagg catgcagcag
    25681 caggaacgcc agcggcgcag tcaggaagat cagtcgtgga atgcccgaca agaagtggaa
    25741 catggcgttg acgtaacata gccgctgagc aaacttcagc cctttaccgg tgagcgggtt
    25801 atcgagacga aagatttgca ccatcccgcg cgcccagcga atacgctgac cgatatgcgc
    25861 cgacagactt tcggtcgcca gccccgccgc ctgcggaata cgcatatatg cagaggtgta
    25921 gccacgacgg tgcaggcgca aagaggtatg cgcatcttca gtcacagttt cgacagcaat
    25981 gccgccaatt tcatccagcg gcttacggcg aatcaccgca caggaaccgc agaagaaagt
    26041 ggcgtcccac atatcgttgc catcctgcac cagaccatag aacagcgtgc cttcgttagg
    26101 tgttttacgg aaacgcccca ggttgcgttc aaacgggtcc ggtgagaaga agtggtgcgg
    26161 cgtctgcatc atcgccagtt gtttttcttt caggaaccag cccatggtca tttgcaggaa
    26221 cgatcgcgtt ggtacgtggt cgcagtcgaa aatcgacacg aactcgcctt tggcatattt
    26281 cagcgcattg ttgatgttgc ccgctttcgc atgttcatga gtggtacggg cgatatattt
    26341 cacccccacg ttttgcgcaa actggcgaaa ctcttccctg ccgccgtcat caaggatcca
    26401 gatgttcagc ttgtctttcg gccagtcgat acccagcgag gcgtaaatgg tatttttcac
    26461 cacgttgaga tcttcgttgt aagtcgggac aaagatatcc accgacggcc acaacgacat
    26521 atctttcggc aatggcaccg gctgacgatt cagcggccat actacctgga agtagccgag
    26581 caccagcaca atccacgcgt acgtttcggc gaacagcaga ataagcccgc acaccaggct
    26641 gaccggatcg tcccagttca gcgtagaggt gtagcgccac cagatataac ggcaagaaac
    26701 ggtcagcgac agcacaatca acattagcgc cgagaagcgc cccggcatcc gccgtacgat
    26761 cagcgctacc ccccacagca gcatcaggaa gataaactgc gccagcgggt taaacggctg
    26821 agtaacgcag attaacgcca gaatcagcga gaaggtgacg atgataccga ggatcaaccg
    26881 ccgcgccccg gcactcaaat ggccgagctc ttttttctca tcaagatgct gtgttttatg
    26941 gctaacgcgc tcaggcagct cgttcatcca ttgatggtaa cgtccacgaa tattctgcag
    27001 acctgaaaat gcccgcctgc gcggtttcgg cgtttctttg cgcgatgcac cgatcagtaa
    27061 ccagcatgtt tgaatgagat aacggaccgg gtccagcgga cgcggacgcg aggcgttgat
    27121 atgcggatac aggtttttat gttctgcgcg aatacgctgc cagcgcgggt gctccagcgg
    27181 aataaaaatc caggccagga tcaaccaaaa acagccgagc gtcgcgctga aagccgacgc
    27241 gccgtgacga cgataatcgc gataacgccc gataagccgc gcgttgaccg gcgggataag
    27301 caaccaccgg gtcaggatac tcatgattta ctcccgactg gcgttttcag cccggaatag
    27361 ttcaacaggc accagttagc cagcgtcagt atctcttcag ccgccagcgc atcactgcga
    27421 tattcaccga ctggttgctt agccgccagg cattcagcca tcgcttcatc acgatgaatg
    27481 agcatcggca gtaatcggcg ctggctttgc aaccaaagct ggtaaatatc gtcctgaact
    27541 tgactgccga tacggaagtc attaatcaaa atatgcgcgc catccggcag cgcctgctga
    27601 tgcagccgga tatggcagtt ggcatccaca ttgacgattg ccagcgagtg atcgcacaaa
    27661 ctcagcagct ggtgggttat ctgcgaggaa tcacgcggta agtcgattaa aattcactgg
    27721 taacgcccgc tggcttttag ttgctgtaag ccggagcaaa tatcgctcag ccgggtttgc
    27781 cagtgctgtg gattttcttg ttcttcaatg ga
//
LOCUS       DAPOZO010000065        27018 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPOZO010000065 DAPOZO010000000
VERSION     DAPOZO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.065"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(83..1348)
                     /gene="puuE"
                     /locus_tag="ROI21_003024"
     CDS             complement(83..1348)
                     /gene="puuE"
                     /locus_tag="ROI21_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDX5111920.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALND"
     gene            complement(1386..2666)
                     /gene="puuB"
                     /locus_tag="ROI21_003025"
     CDS             complement(1386..2666)
                     /gene="puuB"
                     /locus_tag="ROI21_003025"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDX5111921.1"
                     /translation="MTEHTSSYYSASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPVLAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(2668..4155)
                     /gene="puuC"
                     /locus_tag="ROI21_003026"
     CDS             complement(2668..4155)
                     /gene="puuC"
                     /locus_tag="ROI21_003026"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDX5111922.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPICHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVEVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(4305..4862)
                     /gene="puuR"
                     /locus_tag="ROI21_003027"
     CDS             complement(4305..4862)
                     /gene="puuR"
                     /locus_tag="ROI21_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDX5111923.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(4889..5651)
                     /gene="puuD"
                     /locus_tag="ROI21_003028"
                     /pseudo
     CDS             complement(4889..5651)
                     /gene="puuD"
                     /locus_tag="ROI21_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            5863..7281
                     /gene="puuA"
                     /locus_tag="ROI21_003029"
     CDS             5863..7281
                     /gene="puuA"
                     /locus_tag="ROI21_003029"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDX5111924.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            7430..7594
                     /locus_tag="ROI21_003030"
     CDS             7430..7594
                     /locus_tag="ROI21_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111925.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            7584..8969
                     /gene="puuP"
                     /locus_tag="ROI21_003031"
     CDS             7584..8969
                     /gene="puuP"
                     /locus_tag="ROI21_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDX5111926.1"
                     /translation="MAINSPLTIAAQSGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRAPVSET"
     gene            9103..9348
                     /locus_tag="ROI21_003032"
     CDS             9103..9348
                     /locus_tag="ROI21_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDX5111927.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            9661..11304
                     /gene="sapA"
                     /locus_tag="ROI21_003033"
     CDS             9661..11304
                     /gene="sapA"
                     /locus_tag="ROI21_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDX5111928.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            11301..12266
                     /gene="sapB"
                     /locus_tag="ROI21_003034"
     CDS             11301..12266
                     /gene="sapB"
                     /locus_tag="ROI21_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDX5111929.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            12253..13143
                     /gene="sapC"
                     /locus_tag="ROI21_003035"
     CDS             12253..13143
                     /gene="sapC"
                     /locus_tag="ROI21_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDX5111930.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLILGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            13143..14135
                     /gene="sapD"
                     /locus_tag="ROI21_003036"
     CDS             13143..14135
                     /gene="sapD"
                     /locus_tag="ROI21_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDX5111931.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            14137..14943
                     /gene="sapF"
                     /locus_tag="ROI21_003037"
     CDS             14137..14943
                     /gene="sapF"
                     /locus_tag="ROI21_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDX5111932.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(14993..16156)
                     /locus_tag="ROI21_003038"
     CDS             complement(14993..16156)
                     /locus_tag="ROI21_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDX5111933.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(16165..17538)
                     /locus_tag="ROI21_003039"
     CDS             complement(16165..17538)
                     /locus_tag="ROI21_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDX5111934.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQTAFKDVANGLARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWIAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(17542..20649)
                     /locus_tag="ROI21_003040"
     CDS             complement(17542..20649)
                     /locus_tag="ROI21_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDX5111935.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(20649..21770)
                     /locus_tag="ROI21_003041"
     CDS             complement(20649..21770)
                     /locus_tag="ROI21_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDX5111936.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRISAALSAEVRPQVGGIIQKRLFKEGDLVKAGQLLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(21919..22485)
                     /locus_tag="ROI21_003042"
     CDS             complement(21919..22485)
                     /locus_tag="ROI21_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDX5111937.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            22963..23751
                     /gene="fabI"
                     /locus_tag="ROI21_003043"
     CDS             22963..23751
                     /gene="fabI"
                     /locus_tag="ROI21_003043"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDX5111938.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELAKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            23895..25022
                     /locus_tag="ROI21_003044"
     CDS             23895..25022
                     /locus_tag="ROI21_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDX5111939.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAICMDSSNEYKKISALLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            25090..>27018
                     /gene="rnb"
                     /locus_tag="ROI21_003045"
     CDS             25090..>27018
                     /gene="rnb"
                     /locus_tag="ROI21_003045"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDX5111940.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPV"
BASE COUNT         6237 a         7279 c         6928 g         6574 t
ORIGIN      
        1 atgctttgtc cggcatacat aatcaatacg tcgtttgcag gcctgataag cgcagcgcat
       61 cagacattat tgcgttgggc tattaatcgt tcagcgcatc ctgcaaaatt ttcattgccg
      121 catcgaattg cgcatccggg atggtcagcg gatacaggaa acgaatcacg ttgccgtatg
      181 cgccacaggt cagcagaagc agcccctgcg ccagcgcgcg ttgctggatt ttctgtgcaa
      241 tcgccgctga cggctcgccc gtttgcggat cgttaaactc tgccgcaatc atagacccca
      301 gaccgcgtac cgccgcgatg gccggaacgc tttctttggc atcaatcaac gtgtttgtca
      361 agcgctggcc cagttgattc gcgcgttcgc agagcgattc tttgtcgata atgttgagta
      421 ccgcgtgcgc ggcagccacc gccagcgggt tcccggcgta ggtgccaccc aacccgcccg
      481 gcgcgggcgc gtccataata ttcgcgttac cgaccacgcc cgaaagcggc atgccgcccg
      541 cgaggctttt cgccatcgtc attaagtccg gcttatcggc gtagtgatcc atggcaaaca
      601 gtttaccggt acgcgcaaag ccgctttgca cttcatcggc aatcatcaca atgccgtgct
      661 cgtcgcacag gcggcgaatg gcggcaacca gctcttttgg tgcaacgttg aaaccgcctt
      721 cgccctgcac cggttcgaaa ataattgccg ccacctgctt cgcttcaatg tccgatttaa
      781 acaagcgttc gatggcgtcg agagagtcct gcgttgaaac gccatgtaaa tctgacggat
      841 aaggtacgtg atataccgaa ccggggaacg ggccgaagcc gattttgtac ggcgcgacct
      901 ttccggtcag cgccatggtc atatatgtac gaccgtggaa gccgccgcta aacgcaatca
      961 cgccagggcg tccggtatgg gcgcgggcga ttttcaccgc gttctccacc gcttccgcac
     1021 cggtggtgaa gaacgcagtt ttagcctgcc cgctcaccgg ggcaagggcg ttgattttct
     1081 ccgccagggt gacgtagctt tcgtacggca caatctgata cgccgtgtgg gtaaactgtt
     1141 gcaattgctg ctccaccgcc gcgaccagat cagggtggcg atgcccggta ttcagcaccg
     1201 caatgcctgc ggcgaaatcg atgtattcgt tgccctctac gtccttcagc gtagcgtttt
     1261 ctgccgactg ggcgaagaag ttacacatca cgccaacccc gcgtggggta gcagaaagac
     1321 gacgctgatg gaattcattg ttgctcatgt ttcacacctt ttgactcttc actgttaatc
     1381 ggaaattaaa cgcccagacg atcgcgcagg ctgtaataag ccgcgcccat cgcggtaaac
     1441 ggaacgcgca gcgtgcgccc gccggggaac gggtaatgcg gcagattggc gaaggcgtcg
     1501 aaacgctcgg cgtcaccgcg cagcagttca gcaatcaaac gcccggcgag gtgggtacag
     1561 gtgacgccgt ggccgctgta gccctgcatg taatagatgt tggtatcgag gcgaccgaac
     1621 tgcggcatcc gtgaaagggt cagcaggaag ttgcccgtcc agcgatagtc gattttgacg
     1681 cccttcagct gcgggaaggt tttcagcagt ttcggcacca caagacgctc aacgtcatcc
     1741 gggtctcgcg cgccgtagac cacgccgccg ccgtacaaca ggcggttatc ggcggtaaga
     1801 cggtagtaat caagcagata gttacagtct tccacgcagt agtttttcgg gatcagcgaa
     1861 cgggctaaat cttccgacag ccgctcggtg gtgatcacct gcgtgccgca cggcatgctg
     1921 cgtttcgcta gtaccggctc tactttatcg cccagatacg cattcccggc gacaatcaca
     1981 tacttcgccg tcacctgacc attggcagtc cgcaccacgg ctggcgtggt gtgctggatc
     2041 tgcgtcacgg cggaaagctc atacacgcgc cctccgttca ggcggatggc atcggcttca
     2101 ccaatggcga ggtttagcgg gtgaatatgc ccaccgctgt gatccagaag cgcaccagtg
     2161 tagcgatcgc tggcgacctc gcggcgaata gcgttggcgt ccagcagctc cagctgttta
     2221 ttgccgtagc gttcccagtt ctctttctgc tcttcgagtg tggcgagctg tttatcattc
     2281 atcgccacaa acaggccgcc ggggcggtag tcgcagtcaa tctgataacg tttgatacgt
     2341 tcgcggatga tctctccgcc ttcaaacatc atgctgccga gcatacgggc ggtgtccatg
     2401 ccgtagcttt tttcgatcac gtcgatgtcg cggctgtagg agttcacaag ctgcccgcca
     2461 ttgcgtccgc ttgcgccgaa gccgatgcgt gaggcttcga gaaccactac atcaaagccc
     2521 gcttccgcca gatgcagcgc ggaggaaagt ccggtatagc cgccaccgac cacgcaaacg
     2581 tcgcaggaaa tcgactcatt cagcgtgtcg aatggcgcgt atttattcgc actggcggag
     2641 tagtaactgc tggtatgttc ggtcatttca ggcctccagg cttatccaga tagttttcag
     2701 ttcggtgaat ttttcaaggg cgtgcaggga tttgtcgcga ccgttgccgc tctgcttata
     2761 gccgccaaac ggcacggtca tatcgccgtc gttgtagtta ttgacgaaga cggagccggc
     2821 tttcaggcgg cgactcatgc ggtgcgcgcg ggagaggtcg cgcgtccata ctgccgcgcc
     2881 gaggccgtac tggctgtcgt tggcaagctg tagcgcctgt tcttctgatg tgaaacgcgt
     2941 gaccaccagc accggaccga aaatctcttc gcggcttaag gacgcattcg ggtctacctc
     3001 cacaaagatg gtcgggccga tggcggcagc cagctcggcg ttacggccat ccagcagcag
     3061 ttgccctttg ctttcgcctt cccgaataaa gctatggacc gagtcggcgt gggcgcagtc
     3121 gattaaggtg cccatggtgg ttgcgggatc aagtggatgg cccggctgcc agttttgcgc
     3181 ctgctgtttt aacagggcta agaattcatc ggcgatgctc tcttctagca acaaacgcgt
     3241 tccggcgata cacacctggc cctggttgta gaaaatgcct gcggcggtgg cgcttgccgc
     3301 ctgttgcaaa tccgggcagt cggcgaaaac gatgttggcg cttttgccgc ccgcttccag
     3361 ccagacgcgt ttcatgttgc tgtcgcctgc atctttcagc agctgtttcc cggtgcgggt
     3421 tgagccagta aaggcgatgg catcgatatc gttatgacgc gacagcgcct gccccgcttc
     3481 atgaccaaaa cccgtcacca cgttcaacac accatccggc aagcctgcct ctttagccag
     3541 cccggcgaga cgaatcgcac tgagcggtga tttttcagac ggtttcagaa tcacgctgtt
     3601 tcccgccgcc agcgccgggc cgagtttcca gcaagtcagc agcagcggga agttccacgg
     3661 cacgatggcg gcaatcacgc cgaccggttc acgcacgatc atcgccagct catggctact
     3721 ggtggtcgcc acttcgccat acactttgtc gatcgcttcg gcgtaccagc gaatggcgcg
     3781 tgccgcgccg ggaatatcat cacgcaggct gtgacaaatc ggtttgccgg tgtcgagcgt
     3841 ttccagcagc gccagttctt cagcgttagc ttccattaaa tcggcgagtt tattcagcac
     3901 cgctttacgc ttagccggag aagagagtga ccagtcgcca cgttcaaaca cgccgcgtgc
     3961 tgcgctcacc gcacggtcaa tatcgacgct cttgccgcgg gcaatattcg ccagcggtgc
     4021 ctgggtgacc ggatcaacgg tttcaaaagt ttcattttcc gccgcagcag tatattcacc
     4081 gttaataaat aagcgggttt caatggcgag acttaacgct ttatcctgcc agtaagccag
     4141 atgatgaaaa ttcattatga ctcctgtttc acgtctatca gatatatgca gtttgtgaac
     4201 gtcagggcgc agagatcccc gggccgtatg cccgtttatt tctggcgaca ttctttatag
     4261 attgccgggc gcattgcgct tacccggctt acagaacaaa aagattaaaa cgtggtgggc
     4321 gtatgggcgc tgataattcg gcaaatacct gccgacgtat tactgaaact gtgcgggatg
     4381 ccggtattaa tggcataact ttgccccgcg acgaggtggt aatcctgacc attaatcgtc
     4441 agaacaattt caccttccag tacagtgcct atttcctcac cctgatgctt aattctttcc
     4501 ccagtggttg tgcccggctg gtaagtttca aagatcatcg ccagcgtgcg attcgggtta
     4561 ccgttatgaa ccagcttcat tgacacaccc tgactgccca tctcaattaa gtcgtcctga
     4621 ttaatgacga cctgcggctc atcaggtttt tccggctcgg aaaagaattc cgagagtgac
     4681 agaccataca ccttcagcag cttttgcagc gtactgatgg cagggctgac tttatcttgt
     4741 tctatcgtac tgatagcact gtgagtcagc ccggagagtt cggcggcacg acgttgtgaa
     4801 agcccctgct gctggcggat ttccgacaag cgttttcctg gcgccagtcc ctcatcactc
     4861 atatttgcat ttccttaaac tgtagtggtc agagtcgctg tttttcagcg atatggtgct
     4921 gacaagcggt gataaagccc tcgaacaata tacgcgaaag cgcgtactcg ctactgttcc
     4981 attccgggtg ccactgtacg cccagcgcaa aaggatgatt gatgacgcta accgcctcaa
     5041 ctaagccatc cggcgagcga gcttcaacac gcaaccgtgg gctaacgacc ttcgcccctt
     5101 gtccatgcag agagtttacc caaaagttgc tacattcagg taacaacgca gacagtaatc
     5161 ccccctcttc aaccagaact tcatgcgacg gcgcatattg ctgctccacc ggcagttcgg
     5221 gatcttcccg atgttccagc aattcaggct gttcgcacag cttgcgatgc aacgaaccac
     5281 cggttgccac caccagttcc tgtaaacccc ggcagatggc gaaaatgggg atgcgccttt
     5341 cgagtgcggc attaattaac gccatgctca ggatcacgcc cgggatcggc gtcaggctca
     5401 tcgccgtttt caccatatag gtgcggctgc acattgctgg gactaccagg aagataaata
     5461 ccatcgagtt tcggcaaaag ttgttcaagt aatgacggtt ccgccagcgc atgtggcagc
     5521 gcaattggca agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg
     5581 gtcgcatgac ccttaagcct gttcctgcac attacgacac cgataaccgg attattcatt
     5641 atattttcca tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa
     5701 tgacctttat gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa
     5761 tttaacattt gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta
     5821 cgctttgata acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt
     5881 gaagtagaga actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa
     5941 gtgaaacgct acctggagcg ctacccgaat acacaatatg ttgatgtttt acttaccgat
     6001 ttgaatggtt gcttccgtgg aaaacgtatt cctgtttcaa gccttaagaa gctcgagaaa
     6061 gggtgttatt tcccggcctc ggtatttgca atggatattc tgggtaatgt agtagaagaa
     6121 gcgggcctgg gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc
     6181 ttaacccctt ctgccgccga tccagagttt ataggtcaga tgctcctgac catggtcgat
     6241 gaagatggcg ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag
     6301 ctgcgccagc gcggattgtt cccggtcgta gcggtagagc tggagttcta tttactggat
     6361 cgccagcgcg acgctgaagg gtatctgcaa ccgccctgcg cgcccggcac cgatgaccgc
     6421 aatacacaaa gccaggttta ctccgttgat aatctcaacc acttcgccga cgtgctcaat
     6481 gatattgatg agctggcgca gttacagctg attccggcag atggcgcggt cgccgaggcc
     6541 tcgccaggtc agtttgaaat caatctttac cataccgata acgtgctgga agcttgcgat
     6601 gatgcgctgg cactaaaacg cctggtgcgt ctgatggcag aaaagcataa gatgcacgcc
     6661 acttttatgg cgaagccgta tgaagagcac gcgggcagcg ggatgcatat ccatatcagt
     6721 atgcaaaata accgtggcga gaacgtgctt tctgacgcgg aaggcgaaga ttcgccgctg
     6781 ctgaaaaaga tgctcgccgg gatgattgac ctgatgccgt catcaatggc gttgctggca
     6841 ccaaacgtga actcgtatcg ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg
     6901 tggggccata acaaccgcac cgtcgccctg cgtattccct gcggcgatcg ccataatcac
     6961 cgcgtggaat atcgcgtggc gggtgccgat gccaacccgt atctggtgat ggcggcgatt
     7021 tttgccggta ttttgcatgg ccttgataac gaactgccat tacaggaaga agtcgaaggc
     7081 aacgggctgg aacaggaagg cttacccttc ccgattcgcc agagcgatgc cctgggtgag
     7141 tttatcgaga atgatcacct gcgccgctat ttaggcgaac gcttctgcca tgtctatcac
     7201 gcctgtaaaa atgatgagct gttgcagttt gagcgcctca tcacagaaac agaaattgag
     7261 tggatgttga aaaacgcgta ataccccttg cggggttgtt tcatcggcct gagcggagcc
     7321 aggcctgaca tttgctggta atccagacat ttcccggatg tcgcgttatg gagcgcaggc
     7381 ggcaacgggc ttccgttaac gattaagact gtgacgagga aatgagatga tgccgatgat
     7441 taaatccccg catggcgagg gaggctgcgt atgcgcccct cctgcgactg actggactcc
     7501 gccccctttg cttcccttgc taaacaggtt tgatttcagg tcgacacgac cgcaaacctt
     7561 attacgcagg ggaggcagca actatggcta ttaattcacc actgactatt gctgcgcaat
     7621 ctggcaaaac ccgtctgcga aaatcactga aattgtggca ggtggtgatg atgggtctgg
     7681 cctatctcac gccgatgacc gtatttgata cttttggtat tgtctccggc attagcgacg
     7741 gtcacgttcc ggcgtcctat ttgctggcgc tggcgggcgt gctgtttacc gctatcagct
     7801 acggcaaact ggttcgccag tttccggagg ccggttcggc ctatacctac gcgcaaaagt
     7861 cgattaaccc gcacgtcgga tttatggtcg gctggtcatc actgctggat tatctctttt
     7921 tgccaatgat caacgttttg ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc
     7981 cgccgtgggt gtgggtggta accttcgtcg ccattttaac cgccgcgaat ctgaagagcg
     8041 tcaacctggt cgctaacttc aataccctgt ttgtactggt gcaaatctcc atcatggtgg
     8101 tgtttatctt cctggtggtt cagggactgc ataaaggaga aggcgttggc accgtctggt
     8161 cacttcagcc gtttatcagc gagaacgcgc acctgatccc gattattacc ggggcgacga
     8221 ttgtctgttt ctccttcctc ggtttcgatg cggtgaccac gctttcggaa gagacgccgg
     8281 acgccgcacg cgtgatcccg aaagccatct tcctgacggc ggtatacggc ggcgttatct
     8341 ttatcgctgc gtcgttcttt atgcagctgt tctttcccga tatcagccgc tttaaagacc
     8401 cggacgccgc actgcctgaa attgcgctat acgtcggcgg caagctcttc cagtcgattt
     8461 tcctctgtac cacgtttgtg aacacgttag cgtctggcct ggcgtcgcac gccagcgtgt
     8521 cgcgtctgct gtatgtgatg gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg
     8581 tgcacccgaa atggcggact ccggcactga acgtcattat ggtcgggatt gtcgcgttgt
     8641 cagcgctgtt ctttgattta gtcaccgcga cagcattgat taacttcggt gctctggtgg
     8701 catttacctt cgtaaatctg tcggtgttta atcatttctg gcggcgtaaa ggaatgaata
     8761 aaagctggaa ggatcacttc cactatttgc tgatgccgct ggttggcgcg ctgacggtgg
     8821 gtgtgctgtg ggttaatctc gagtcaacgt cactgacact cggtctggta tgggcttcgt
     8881 taggcggcgc atatttgtgg tatctgatcc gccgctatcg caaagtgccg ttgtatgatg
     8941 gtgacagagc gccggtgagc gaaacgtaat agtcggatgc gcggcgcatt atcctgcctg
     9001 agggtaatgc accgcgccat taactataaa aagatgtatg acccggatca cgagtgctat
     9061 catccgccct tatgcttagc gcattaaccc cagggggaaa taatgaacca cgatatcccg
     9121 ctaaaatatt ttgatattgc cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagac
     9181 gctgaacgca caccgctggc cctctacttc cagttactgc tcacccgttt gatgaacaat
     9241 gaagagatca gcgaagaagc ccaacatgaa atggctgctg aagcggggat taatcccgta
     9301 cgcattgatg agatcgcaga gtttttgaat caatggggta atgagtaatt atttcattgc
     9361 agacgctcgg tcagtatcgt tgcccggata aagcgttcac gccgcatccg gctaacaatg
     9421 acgcgcactg cagcgtgctt taatgggttt ctcacgatcg taccgctctg ctcctcgcta
     9481 agttgttctg tgagttatag ttatagagaa atcacgggta cgaatgttga acagatggcg
     9541 acgtcacctg ttcaacgacc aaccacacgg ccctcattac gacccctgaa cacaaacggt
     9601 aatggcaaat gtcattaaat attagtaaga tagcctcttt acataaaatc cattaatatt
     9661 atgcgccagg tattatcgtc tcttttggtg attgctggac ttgtgagtgg tcaggcaatc
     9721 gccgcgcctg aatccccccc gcatgctgat atccgcgata gcggttttgt ctattgcgtc
     9781 agcgggcaag tcaacacctt taacccatcc aaagcgagca gtgggttaat tgtcgatacc
     9841 cttgccgccc agttttatga tcgactgctg gatgtcgatc cctataccta tcgcctgatg
     9901 ccggaacttg ccgaaagctg ggaagttctc gacaacggcg cgacctatcg cttccacctg
     9961 cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc
    10021 gacgatgtgg tgtttacctt ccagcgaatt tttgaccgca acaacccgtg gcataacgtc
    10081 aacggcagca acttccccta cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc
    10141 cgcaaactgg ataatcatac ggttgagttc cgactcgccc agccggatgc ttcttttttg
    10201 tggcacctgg cgacccatta tgcatcggtc atgtcggcag aatatgcccg gaagttagag
    10261 aaagaagatc gtcaggaaca actcgaccgt cagccggtcg gcactggacc gtatcagttg
    10321 tcggaatacc gcgccgggca atatattcgc ttacaacgtc atgatgattt ctggcgtggt
    10381 aagccgttga tgccacaagt ggtggtggat ttaggctctg gcggtaccgg acgtctgtcg
    10441 aaactcctga ccggggagtg cgacgttctg gcctggcctg ctgccagtca gctatccatt
    10501 ttgcgtgacg acccacgctt gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg
    10561 gcattcaaca ccgccaaacc gccgctaaat aatcccgctg tccgccatgc gctggcactg
    10621 gcgattaata accagcgtct gatgcaatcc atctattatg gtacggctga aacggcagcc
    10681 tctattttac cgcgcgcctc gtgggcctat gacaacgagg ctaaaattac tgaatataat
    10741 ccggcgaaat cgcgcgaaca gttgaaggcg ttggggctgg aaaacttaac gctgaaactg
    10801 tgggtaccaa cacgttcgca ggcgtggaac cccagtccac tgaaaactgc cgaattgatt
    10861 caggcggata tggcacaggt tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt
    10921 caggaggcgc ggttgatgga tatgagccat gatctgacgt tatccggttg ggcgactgac
    10981 agtaacgacc cggacagttt cttccgtccg ttactgagct gcgcggcaat tcattcgcag
    11041 accaacctcg cccactggtg cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc
    11101 tcgcagcagc tggcggcgcg tattgaagcc tatgacgaag cgcagagtat tctggcgcag
    11161 gaattaccca ttttgccgct ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc
    11221 aaaggtctgg tacttagccc gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa
    11281 caggatgagg tgaaaaaacc atgattatct tcaccttacg ccgcattttg ttattgattg
    11341 tcaccttgtt cctgctgacc ttcgttggct tcagcttgag ctatttcact ccacacgcgc
    11401 cgttacaagg cgcgtcactg tggaatgcct gggtgttctg gtttaatggc ctgatccact
    11461 gggattttgg tgtttccagc attaacggtc aaccgattgc cgaacagtta aaagaggtct
    11521 tcccggcgac gatggaactg tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc
    11581 ccgtggggat gattgccggg attacgcgcc ataagtggca ggataacttg atcaacgcca
    11641 tcgccttact gggcttttca atcccggtgt tctggctggc gcttctgttg acgctgtttt
    11701 gttcactcac gctaggttgg ttgcccgttt cagggcgttt cgatctgctc tacgaagtga
    11761 aaccgattac cggttttgcg ttgattgatg cctggctttc ggactcacca tggcgggatg
    11821 aaatggtcat gagcgcaatc cgccatatga tattgcccgt gatcaccctg tcggttgcgc
    11881 caacaactga agtgatccga ctgatgcgta ttagcactat cgaagtgtat gaccagaact
    11941 atgttaaagc agcggcgacc cgcggtttgt cgcgctttac tattttgcgt cgccacgttc
    12001 tgcataacgc gttacctccg gttattcctc gcctgggctt acagttttct accatgctga
    12061 cgctggcgat gatcaccgaa atggttttta gctggccggg tttagggcgc tggttaatta
    12121 acgccattcg ccagcaggat tacgcagcca tttccgccgg agtgatggtg tgtggctcac
    12181 tggtcattat tgttaacgtg atttctgata ttttgggtgc catggctaac cctctgaaac
    12241 ataaggaatg gtatgcctta cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg
    12301 cgtaccgcct ggcgcaaatt ttatagtgac gcctctgcga tggtcggcct gtacggctgc
    12361 gcggggctgg ctgtactgtg tatttttggc ggctggtttg cgccttacgg catcgatcag
    12421 caatttctcg gttatcaatt attgccgccg tcatggtcgc gctatggcga agtttctttc
    12481 ttccttggga ctgacgacct cggacgcgat gttttaagcc gtttactgag cggagctgcg
    12541 ccaaccgtag gcggcgcatt tgtggtgacg cttgccgcga cgatctgcgg cctgatactg
    12601 ggcaccttcg ccggggcgac ccacggccta cgctcagcgg tgcttaacca tattctggat
    12661 accttactgg cgatcccttc gctgctgctg gcaattatcg ttgtagcgtt tgccggaccg
    12721 agtttgtctc acgccatgtt tgccgtctgg ctggcgctgc tgcctcgtat ggtacgttcg
    12781 atttacagca tggtgcatga cgaactggaa aaagagtacg ttatcgccgc ccgtctggat
    12841 ggcgcatcaa cgctgaatat tctctggttt gctgtgatgc caaacatcac cgctgggctg
    12901 gtcaccgaga tcacccgcgc actgtcgatg gcaattctcg atatcgccgc gctgggcttt
    12961 ctcgatctcg gcgcacaact cccctcgcct gaatggggag cgatgctcgg tgatgcgctg
    13021 gaactgattt atgtcgcccc gtggactgtc atgctgccag gtgcggcaat tatgattagc
    13081 gtgctgttgg ttaacctcct tggcgacggt gtccgtcgtg caattattgc gggggtggaa
    13141 taatgccatt actcgatatt cgtaacctga ccattgaatt taaaaccggt gatgagtggg
    13201 ttaaagccgt cgaccgcgta agtatgacct taaccgaagg tgaaatccgc ggtcttgttg
    13261 gcgaatcagg ttccggcaaa agtttgattg cgaaagcgat ttgtggggtg aataaagata
    13321 actggcgtgt tactgctgac cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg
    13381 cacgcgaacg gcgcaagctg gtaggtcaca acgtgtcgat gattttccag gaaccgcagt
    13441 cgtgtcttga tccttcagaa cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga
    13501 cctataaagg ccgttggtgg cagcgttttg gctggcgcaa acgccgtgcc attgaactgc
    13561 tgcaccgcgt ggggattaaa gatcacaaag atgcgatgcg cagttttccc tatgagttga
    13621 ccgaaggtga atgtcagaaa gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc
    13681 tgattgctga cgaaccgaca aactcaatgg agccaacaac ccaggcgcaa atctttcgcc
    13741 tcctgacgcg tctcaaccaa aacagcaata ccactatttt gcttatcagc catgacttac
    13801 aaatgcttag ccaatgggcg gataaaatta acgtgcttta ctgcggtcaa acggtggaaa
    13861 ccgcgccaag taaggagttg gtgacaatgc cacatcatcc ttatacccag gcgctgatcc
    13921 gcgcgatacc agacttcggc agcgcgatgc cgcataaaag tcgcctcaat acgctgcccg
    13981 gcgctatccc actgctggaa cagttaccga ttgggtgtcg tctggggccg cgttgcccgt
    14041 atgcacaacg agaatgcatt gtgacgccac gtttgacggg ggcgaaaaat catctctatg
    14101 cctgtcattt cccgctgaac atggagaaag aatgagatga tcgaaacgct gcttgaagtg
    14161 cgtaatttaa gtaaaacgtt ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa
    14221 gcggtaaaac ccttgagctt tacgctacgt gaaggccaga cactggcgat tattggcgag
    14281 aatggttcgg gtaaatccac gctggcaaaa atgctggcgg gaatgataga acctaccagc
    14341 ggcgagttat taattgacga tcatccgctg cattttggcg attattcctt ccgtagtcag
    14401 cgtattcgca tgatttttca ggatccttcg acatcgttga atccccgtca acgtatctcg
    14461 caaatcctgg attttccact gcgtctgaac accgacctgg aaccggaaca gcgtcgcaaa
    14521 cagattattg aaacgatgcg tatggttggg ctgctaccgg atcacgtcag ttactatccg
    14581 catatgttgg cacccgggca aaaacagcgt ctggggctgg cgcgcgcgct gatattgcgc
    14641 ccaaaagtca ttattgccga tgaagccctc gcctcactgg atatgtcgat gcgttcgcag
    14701 ttgattaatc tgatgcttga gttacaggaa aaacagggca tttcgtatat ttatgttacc
    14761 cagcatatcg gaatgatgaa gcacattagc gaccaggtgc tggtgatgca tcagggcgag
    14821 gttgtcgagc gaggcagcac cgccgatgtg ctggcatcgc cgctgcatga actcaccaaa
    14881 cggctgatcg ccggtcattt tggcgaggca ttgacggcgg atgcgtggcg taaggaccgt
    14941 tagtgttttg cacctcatcc tgacaggggc ccccctcagg atgaggtgtt cattactttt
    15001 gattgtcgat tctggtgaga gcaaccagca atgccagcgt ataacacacc gtaattgcca
    15061 gactcatttt taacagtgtt tctccgccaa ttcccgacaa tggcgcggct ataccgccga
    15121 agacaaacat gctcatcccc atcaacgcag acgccgttcc agattcctgc gtccctactg
    15181 cactcatagc ctctgccccg ccaacagtgc cgatgccgct acaaaatgcg atggtgaaaa
    15241 ataatgccac cagtgccgga atgggcaatt gtatccatgc gcataagacc gtgagcaatg
    15301 cacacaaaat tgccgctatc aggccacctc gcaacaatgt catcgcgtta atccgtcgag
    15361 ccaggcgcga aaagatccat gaagcaatga taagtccgat gccgttaagg ccaaacacca
    15421 ggctaaattg cattggacta aagccaaatt ccttctgcaa cacgaacgac gaagagccga
    15481 tatatgcaaa caagcctgcc agcataaaac tttgaatcag gcaaaagcgc ataaaacggc
    15541 ggttctgcac cacgcttcgt ccggttaata gcaatgatga gcccctttta ttttctggca
    15601 aggtctcatt aataaataac acgcagccca gcaacaatac ggtgctaatt tcagccatta
    15661 cccagaataa agtgcgccag tcaaaagtgc taacaatgta cccgcccagc actggcgaca
    15721 acaccggtgc caggccattc accgtcatta acagcgcaaa aaactgggtc aacgttactc
    15781 cctgatattt gtcacgagca atagaacgag agagcaccga accacccgcc cccgcgatcc
    15841 cttgaataaa acgccagacc accagccagt aaatattgtt cgttgtcgcg cacaaaatgg
    15901 aagaaacaat aaacaatagc aacgacaaga ttaacggacg tttacgccct attttgtcgc
    15961 tcagagggcc aaataacagt tgtccgacgc caagaccgat cagtgatgcg gtcagggtta
    16021 attgcgttat ggtcgtggtt gccgccagtt gctcagacat ctccggcaaa gccggcaaat
    16081 aaagatcggt acacatcggg cctataccgg ctaaaagacc aagaatcaat gcccatgaaa
    16141 gagagactct agccatatta ttccttactt gagtgatgac atgccgccgc ccagcgactg
    16201 ccagagcgtg attcggtttg taaaatcagt cagttgcagt gccagcagct cctgctgtgc
    16261 gatccacaag ctgcgctgcg ccgtcaacac ggtcaggtaa tcaccaacac ccgcctgata
    16321 gcgacgcaag ccaacatcga ccgtttgttg ctcagcttta acatactggc gttgtgcatc
    16381 cagttgctct tcaagcgtgg tccgccgcgc cagtccgtta gcgacatctt taaatgcggt
    16441 ctgaacggtt ttttcatagg tggcaatgag cccacgcttt tgtgcttctg cgtaacgaag
    16501 ctgcgccaga ttacttccgc cagtgaacag cggtaatgtg accgatggcg caaatgacca
    16561 gatctgcatt ccgtggctga acaacgacga caatgcgtca ctgccaacac cggcactggc
    16621 agtcagcgaa atcgtcggga aaaagttagc ccgcgccgcg ccaatatctg cattggcgct
    16681 cttcaaatta tgttccgctt cctggatatc cgggcgacgc agcagaacat cagatgatac
    16741 tcccgcagga accagactta tcatttgttc cggcaggctt tccagtgttc ccggtagcag
    16801 attttccgca agcgttgtac ctgccagtaa gttcagcgca tttttatcct gcatcacttg
    16861 tgtctgatag ctggcaacgc tggcgcgcgc ttgctgataa acactcatcg cctcactgac
    16921 atctgtcgct gccgccgtgc caacctgttg ctggcgctgg ataattttca atgagttttc
    16981 tgcactggcc atcgtttctt ttgccagggc caggttgctg ttatcagccg ccagtgtcag
    17041 ccaggcggtg ctgatttcag caatcagcgt taaacgagtg ttctgggctg taaattcact
    17101 ggcaagccag gtttcccgcg ccgcccgaga taaactctga ttacgaccaa acagatctaa
    17161 cgtataactg gacaccgtgc cgtcagcctc ggcagttgtt cccgtaccat tcgccagcga
    17221 acgactgcga gtcgaactta aggccgcatt caccgtcgga aataacgacg cattagtttg
    17281 cccatagagc gcccgtgctg agtcaatatc agcaatcgct ttttgcacat cgcggttact
    17341 gttcagtgca atggttacta cctgctgcaa tcggggatcg tggatcacct gctgccagtc
    17401 atggctgatt gccttcccct ggccctgtgc tcccggcaag gtcgccggga ttggggattc
    17461 aggcgtactg tagtgaggat cgagcgagac acatcccgca ctcaacaaca ccagaaaaat
    17521 aagagaacga cgcaacataa cttactcctg acggcgcggt ttaccggcaa acaaacgttt
    17581 caccagtaca aaaaacagag gaacgaagaa aatggccagt aatgtagcgg tcagcgtgcc
    17641 gccaataatg cccgtaccaa tggcgatgcg gctgttcgcc cctgcgccgg ttgctatcgc
    17701 cagcggcatt acccccgcaa taaacgccag cgaggtcatg atgattgggc gtaaacgagt
    17761 ctgcgccgcg cgtaatgccg cacgactcaa ggaatagccc tctgcaaccg ccgcttcagc
    17821 aaattccaca atcagaatgg cgtttttcga cgacaagccg atagtagtta atagcgctac
    17881 ctggaagtaa acgtcgttgt ttaaatcacg catccaggcc gccagcgccg cgccaagaag
    17941 ccccagaggg atcaccagaa tgaccgagaa cgggactgac cagctttcgt acagtgctgc
    18001 aaggcagagg aaaaccacca aaatagaaac tgcatacaga cttaatgcct gaccgctggc
    18061 cagtttctcc tgcaatgaca aaccactcca ggcccatgtt gttcccgcag gaagactgtt
    18121 tgccagttcc tccatttttg tcattgcatc gccggaacta aagccagtcg cgttttcgcc
    18181 ctgaatttca taggctgccg agccgttgta gcgcaccagt ctttccggtc catacagcca
    18241 gcgggtggtc gcaaaagcag agaatggtgt catggcgtta tcgctaccgc gcacaaacca
    18301 tttacctaag tctgacggcg cggaacgaaa ttcactgtcg ccctgaatgt agactttttt
    18361 cacacgacca cggtcaataa agtcattcac ataagtaccg ccccacgcac tggacagggt
    18421 gtcggtgaca tcattcaaac tcaaccctaa tgacaccgct ttattactat caatatctac
    18481 ctgcaattgc ggcatttgtg gcaaatcatt ggcacgcaca gaatgtaact ccggactttg
    18541 attcgctttt tctatcaact gattacgcat ttgcagtagt gtttcgcgat cggttccacc
    18601 gttagctaac aattcaaacg taaaaccatt gctttgcccc agcccatcaa cggctggcgg
    18661 cgtcatcgca aataccgtgg catcacgaat tgtgcccagc tctttggttg cccgtagggc
    18721 gatagcctgg gcggtgtttt ctgccccttt acgttgagac cagtttttca acgaaacaaa
    18781 cgccatcccg gtgttctgtc cgctaccgct aaaactgaaa ccatcaacgg taaagatgac
    18841 atcggtattt gctttctcgt taatcaggaa ccagtcaaca atctggcgat tcacttctgc
    18901 tgtacgggca gcggtcgccc cagccggcag cgtgtactgc accatgattt cgccctggtc
    18961 ttcagtgggt aagaaactgc ccggcagttt ccacatcatc agggccattc caccacccag
    19021 cacgacataa agccccatcg ttcgggctgc acggcgcagg acataaatta cgcctcgctg
    19081 atatttatct tcagtacggc ggtaaaagcg gttaaatgcg ccgaaaaagc cttttttatg
    19141 tggcggaaca tgctggagga cggaaccgca cagggcggga gtcaaggtca atgccactac
    19201 cacggaaagc agcattgcgg agataatggt tatcgaaaac tgacgataaa ttacccccgt
    19261 agatccccca aagaaggcca taggcaggaa tacggctgac aacaccagcg caatggcaac
    19321 cagtgcgcca gaaatctcgc ccattgattt ttccgtggct tcacgcgcgg gcaacccttc
    19381 gtcacgcata atgcgttcga cgttctccac caccacaatg gcatcgtcca ccagcaagcc
    19441 gatcgccagc accattgcaa acaacgtcag ggtattaatc gaatagccaa acaacgcaag
    19501 aacgccgaat gtgcccagca aaacgacggg gacagctaac gccggaatca gtgtggcacg
    19561 caggttctgc aggaataaat acatcacgca aaccaccaat acgatggctt caaacagcgt
    19621 ctgaattacg tcctctacag agattttaat aaattcagtg ctgtctttcg ggtatgcgat
    19681 gtcgtatccc tgcggcatgt tccgctggaa ttcggcaatc ttatccttga ccagcgtcgc
    19741 cgtattcagc gcattcgcac cgggcgaaag cattacagca accccggcag acgggtgacc
    19801 gttaagtttg ccgatagcgg tataatcttc actgcccatc tcaacgcgag ccacatcttt
    19861 tatacgaaca actgcgccgt ctgactggct tttcacgata atatttttga actgatccac
    19921 cgtctgcaag cgagactgcg cacgtaccgt tgcggtcagt tgctgagtat tcggtgaagg
    19981 caatgcccct attttcccgg cagtaacctg cacgttttgc gcttcaatag cactttgcac
    20041 gtctgaaggc attagcgagt aagacgccag tttggccggg tcaagccaga tgcgcatagc
    20101 gtattccgcg ccaaagactt gcagactccc cacaccttca acacgcgcca gcgggtcctg
    20161 aacgttacta accaaccagt cggcgatatc cgaactggag gctttgtcag tggtgtcata
    20221 cacggcagca atcagcaaaa agttgctttg tgatttctcc accgtaacac ccgtttgctg
    20281 cacttcgctg ggtaggcgcg actccgcctg ctgaatttta ttctgcacct gaacctgtgc
    20341 agtatctgga tcggtacctt gttcaaaggt cacattaata ctgaccgaac catcagagct
    20401 gctggttgag ctgaaataga gtaaattatc aagcccagtg agttgttgct cgataacctg
    20461 agtcacgctg ttctccagcg tttcggcaga agcaccagta taagtggctg aaattttaat
    20521 ggtcggtggc gcaacgtcag gatattgcgc gacgggcaat gtgcgaatag ccagaatccc
    20581 cgccaacata atcaaaatgg cgataaccca ggcaaagacc gggcggcgaa caaaaaagcg
    20641 cgaaaacatc aggcgttgcc tccattagcc tgaacttcaa cagccttgac ggtctgccct
    20701 gaagtgactt tggcagaacc ttcaacaatc agtcggtcgc cgttgtgcag gccgttcagc
    20761 accagccatt tatcaccata cgtttctccc gtttcgagcg ttcgctgctc tactttattg
    20821 tctttattca ccaccagcgc agttgcattg cctttagcat cgcgcgtgac gccctgttgc
    20881 ggcgcgagaa tagcgtcttc catcacgcct tcatcgacac gagcgcgaac aaacattccc
    20941 ggtaataact gctgttgtgg attggggaaa atcgcccgta atgtcaccga accggtagac
    21001 tcatctacag cgacttcggt gagttccaga cgcccttttt cgctgtaggt tgtgccatct
    21061 tccagaataa gtgagacgct catggtgtca ctgttggtcg ccagtgactg tttacgcaga
    21121 cgtaataaat cgacactgga gcgagtgagg tcgacataca ttgtatccag accacgaata
    21181 gtcgttaacg ctgtatcttg cgacgcggtc accagtgcgc caggggttac cgacgaaatg
    21241 ccaatgcgcc ctgaaatcgg tgcggttacc gtggtccagt caagattaat gcgcgcagtt
    21301 tccagtgcgg cttttttcgc ctctacactg gctttatctt gtgcacaggt agactgagca
    21361 tcatcagcat cctgttgtga aacaccgttc tctttcacca gtcgggcata acgctgcgct
    21421 ttctggcaat cggcttttac cagtgcctgt gcttgttgta atgctgcccg agcttcattc
    21481 catgcagcct gataactggc cgcatcaatc tggtagagca gctgtccagc cttgaccaga
    21541 tcaccttcct taaataagcg tttctggata attcccccaa cctgcggacg tacttcggca
    21601 ctgagcgcag cactgatgcg tccggttaat tcactgacta ccgagaccgg ttgagatttg
    21661 actgtaacaa cgccgacttc tgtttctgac ggggatgaat tgttactttg cgtattatca
    21721 caaccagata agagcagcat tgccactaca gatgttgcta tatacttcat tattttctcc
    21781 agggtgagaa ataccctcct cattaaaatt aatgaggata tgtcagggat attcagttat
    21841 aacgaacccg catcgcgaaa tgaatttcgc aatgaaataa gggggattaa tatcccccgc
    21901 tattggttaa ttctgttctt atttatttat caacatacta acaatatcct gatatatttc
    21961 ctgcagatgt tgtggatcgc ttttttgcgg tgttaaacga cgataaaccg taccttccat
    22021 catggtagca aacacttcaa cgcagcaacg aatgtgctcg tcgctaagat ggggatgcat
    22081 tcttttcata tgtgcacaac cgttagcgaa cattctggcg tcagcttctt caagcattgc
    22141 cataacccgc gggttgcgtg tggcttctgc agccacttcc agcatcagcg cgtcgtcgtc
    22201 ctcatttaag gatttacgca aggcaaggac ttccgggaag tgatcggtac gcgcgtcaat
    22261 gtccatttgg gcaatacgaa agtcgatgat acggcgaacc atctcttcaa taatggcgtc
    22321 tttgttggcg aagtaacgat atatctgacc cacgctaagc tgagcttcgc tggcaatttg
    22381 cgacatactg gcggcatgaa acccgcagcg acgaaaacac cgacgggcgg cgttaatgat
    22441 ttcatcctga cgctgtttgt ggcgtatctc cagcttgctg gtcatttctg tctcctggcc
    22501 tatcacttgg tgagaatgat cgttctcatt tttgctgatt atacgtttct ccaaagtaat
    22561 ggaaagtatt tttatcttat tcatggtgca tattctgata atgaagagat tgtggagatt
    22621 atgtgaagat agttgacaat attaagttgg ggtcaattca tcagaaaggc tgctacttgt
    22681 tgtcgccagt tctgttttca tcatcaaact tatcggagct aatacgaata gtttcatagt
    22741 cagattctat aagatattcc cccaacctac cccttgcagg cgtcaagagt agataacgca
    22801 aaatgctctg taccgcagct tctctccggt acagaaagcg caactatagc cacccacagc
    22861 caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga cgaatcgcct
    22921 gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt tctttccggt
    22981 aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg tatcgctcag
    23041 gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa actgaaaggc
    23101 cgcgtagaag aatttgcagc tcaattgggt tctgacatcg ttctgcagtg cgatgttgcc
    23161 gaagatgcca gcatcgacac catgttcgct gaactggcga aagtttggcc gaaatttgac
    23221 ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga ctatgttaac
    23281 gccgttaccc gtgaaggctt caaaattgct cacgacatca gctcctacag cttcgttgca
    23341 atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac cctttcctac
    23401 cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa agcgtctctg
    23461 gaagcaaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc
    23521 atctctgcgg gtccgatccg tactctggcg gcttccggta tcaaagactt ccgcaaaatg
    23581 ctggctcatt gcgaagccgt taccccgatt cgccgtaccg ttaccattga agatgtgggt
    23641 aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga agtggtccac
    23701 gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata atcgttctgt
    23761 tggtcaagac gggcggcgtt ctgccgcccg ttatctctgt tatacctttc tgatatttgt
    23821 tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat ttcctttacg
    23881 accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg tatcatgaaa
    23941 cgcaatccag tactgcggaa tatgacgttc tgcctctggt cccggaagcc gcaaaggtca
    24001 gcgatccctt tctgctcgac gtgatccttg atgaagaaac gctggccccc ttcctttcat
    24061 ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt accgtgaccc
    24121 gttcacagac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt gctcaggttt
    24181 gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc cccgggcctg
    24241 attcctccag ggaaagtaat catcggttag cacaaatcac gcaatatgcc cgccaactgg
    24301 ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac gtcggtctta
    24361 ctgtctggga ctgtgtgatc attaatcaaa tcattggttt tattggcttt caggcgcgga
    24421 ccattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc ggtctggaga
    24481 tacaaaacta tgccgacgcg tcactgtttg ctgatgagtc aatacgctgg cgaagcagct
    24541 atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa ctttgccaac
    24601 tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag ttgctaaaca
    24661 gcacacgggt caatacacag ccggataatc agcttgcggc gttgttatgc gcgcggataa
    24721 atggcagtcc tgcttgtttt gccatctgta tggattcatc aaatgaatat aaaaaaatca
    24781 gcgcccttct gcgcaagggc gaaaatgaaa ttaaccaatg ggctgaccgt cattctgttg
    24841 agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc tttagcgccg
    24901 cccagttcag cccattactc gaacacgaaa aatcatcaac gcagattatt aatctgctgg
    24961 tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt gagacatatt
    25021 aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg gccaccaaaa
    25081 tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca actgcattcc
    25141 cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg cttcctggaa
    25201 gtcgacgcgc aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt catgcatggc
    25261 gaccgaatta tcgcggttat ccacagtgaa aaagaacgtg aatccgcaga gccagaagaa
    25321 ctggttgaac cgtttctgac tcgtttcgtg ggtaaagttc agggcaaaaa tgaccgtctg
    25381 gccatcgttc ctgatcatcc actcttaaaa gacgccattc cttgccgcgc agcccgtggc
    25441 ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg tcatccgctg
    25501 aaaggcgatc gttctttcta tgctgaacta acacaataca tcacttttgg tgacgaccac
    25561 tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc accagacggc
    25621 gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgaccgc gctggatttt
    25681 gtcaccatcg acagtgccag cacagaagat atggatgacg cccttttcgc taaggcgttg
    25741 ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg gattgctgaa
    25801 ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct gcctggcttc
    25861 aacatcccta tgcttcctcg cgagctttct gacgatctct gctcactgcg cgccaatgaa
    25921 gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat tgaagataat
    25981 atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga ccaggtctct
    26041 gactggctgg aaaataccgg tgactggcag cctgaaagtg aagcaattgc cgaacaagtc
    26101 cgtttgttag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca cgcactggta
    26161 tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt gctggatatc
    26221 gtcgccgagc ctcgtcgcat tgccaatcgt atcgtcgaag aagcgatgat tgccgctaac
    26281 atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa cgtgcatatg
    26341 ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaaccca cggtctgcat
    26401 gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg tgaactggat
    26461 gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt tgctgaaatt
    26521 agcactgaac ccggtcctca ctttggcctc ggtctggagg catacgccac ctggacttcg
    26581 ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt tatcaaaggc
    26641 gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgagcg tcgccgtctc
    26701 aaccgaatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct gaaagacaaa
    26761 gccgggaccg acacccgttt cgcggcggaa attgtcgata tcagccgtgg cggcatgcgt
    26821 gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt acacgctgtg
    26881 cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg tgaaacggct
    26941 tacaaagtga ctgacgttat tgacgtcacc attgccgaag tccgcatgga aacccgcagc
    27001 attattgcgc gcccggtc
//
LOCUS       DAPOZO010000066        26914 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPOZO010000066 DAPOZO010000000
VERSION     DAPOZO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.066"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..627)
                     /gene="upp"
                     /locus_tag="ROI21_003046"
     CDS             complement(1..627)
                     /gene="upp"
                     /locus_tag="ROI21_003046"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDX5111941.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            952..1989
                     /gene="purM"
                     /locus_tag="ROI21_003047"
     CDS             952..1989
                     /gene="purM"
                     /locus_tag="ROI21_003047"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDX5111942.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            1989..2627
                     /gene="purN"
                     /locus_tag="ROI21_003048"
     CDS             1989..2627
                     /gene="purN"
                     /locus_tag="ROI21_003048"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDX5111943.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTARAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2798..4864
                     /gene="ppk1"
                     /locus_tag="ROI21_003049"
     CDS             2798..4864
                     /gene="ppk1"
                     /locus_tag="ROI21_003049"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDX5111944.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4869..6410
                     /gene="ppx"
                     /locus_tag="ROI21_003050"
     CDS             4869..6410
                     /gene="ppx"
                     /locus_tag="ROI21_003050"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDX5111945.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6449..8692)
                     /gene="pdeF"
                     /locus_tag="ROI21_003051"
     CDS             complement(6449..8692)
                     /gene="pdeF"
                     /locus_tag="ROI21_003051"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDX5111946.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRAVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVARRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(8874..9026)
                     /locus_tag="ROI21_003052"
     CDS             complement(8874..9026)
                     /locus_tag="ROI21_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111947.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            9044..9235
                     /locus_tag="ROI21_003053"
     CDS             9044..9235
                     /locus_tag="ROI21_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDX5111948.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            9546..10064
                     /locus_tag="ROI21_003054"
     CDS             9546..10064
                     /locus_tag="ROI21_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDX5111949.1"
                     /translation="MMKFKKCLLPVVMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            10080..10619
                     /locus_tag="ROI21_003055"
     CDS             10080..10619
                     /locus_tag="ROI21_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDX5111950.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRATTAAANKKARQDKLDAEASADKKRDQSYQ
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(10714..12291)
                     /gene="guaA"
                     /locus_tag="ROI21_003056"
     CDS             complement(10714..12291)
                     /gene="guaA"
                     /locus_tag="ROI21_003056"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDX5111951.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(12360..13826)
                     /gene="guaB"
                     /locus_tag="ROI21_003057"
     CDS             complement(12360..13826)
                     /gene="guaB"
                     /locus_tag="ROI21_003057"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDX5111952.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            13988..15364
                     /gene="xseA"
                     /locus_tag="ROI21_003058"
     CDS             13988..15364
                     /gene="xseA"
                     /locus_tag="ROI21_003058"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDX5111953.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKTKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIFPTAVQGDDAPGQIVRAIEMANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKAGDTLTTRLGDGVVISEVSAVTKTRK
                     PRKKAANP"
     gene            complement(15424..23466)
                     /locus_tag="ROI21_003059"
     CDS             complement(15424..23466)
                     /locus_tag="ROI21_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RatA-like protein"
                     /protein_id="HDX5111954.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPAKTHEVAFTAKPSEFSADISVSKLLVTNPQNREGDIIST
                     PRWENQTPPTVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLVAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKTTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRLGVVNNTAPVKLGTTELTTTATEYRGSTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQASETAVIFTVLTSPDVPQATMWGHMPDTL
                     KALDYTFSRPKLAAEVDNEDGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNYYWSSLAGMSGQHHAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGVNR
                     KNQTDAAWEAYPVQIDGGSNVRKVDAHTYEGITDANGEASLTLTQPGGVGVKTHITAR
                     MRSDFTASDEKDVIFTVITSPDTDKARMWGHMLGIIEANNIFKRPRLADETANELGAV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANVIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDHDVSTSSRAQAKVG
                     ETITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQPQGVGLATPLNIAPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVSSPDGTLTENNEVWSRVSQANTSSTSKG
                     GCGANMLPRRSQLNALYDANSGNAVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDMPFTLKRGDGYTRSNEKHIAGSGDALVAPVVINSGLADETSLNDTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTAALYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAVDGTVFKRPLLLKELSSTSGRTAIAEDNEDWAQFTQTQATSTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDSTNNVVKAKKGEEVVVRVTTKDSQ
                     GNPVGNTAFTLKRNLSVNRANASTTVTAGALIVTDAWGNTQSNFSSTTALIYGVTGAD
                     GTTTLALKQDNTTGLKTELTAMLDTDNNVKSMLPVVFTVITSPDTPKAKFWGHMAETM
                     TGAGGLIYKRPLLQDELSVTTSRSSFQEDGESWSLFSPDQSNNTSVNGCGAGYVPIES
                     ELESLYADEGYVPIHDVTGWPVSRAYISSTVVAYYTQTFNFKAVSLKTGDGTEVMSSS
                     LGLLSCRATPVAVTSHIIVEANDATQFVKVDETLSALKRKKGDDAVIRVVTKDAQGNV
                     VPNVPFILKREGSTNRQNVQLSNRTITVINAAGTSARVDTPSISLYAVTGADGTATFT
                     VKQDDSIGLVTNVYAQAYQSSLESNKLPVMFTVITSPDTPLASYWGHMAETFTTRSGT
                     AFKRPLLSAERSSGQSFIEDNEEWAVLRSATKGDIDKSGCDVHYQPLLSELQALYDEH
                     PSRAIKTDLGIPVNSYWWAYDMVAYAGNWYDQYIYLLNGSSGRASSTTSALMLCLVNP
                     HPEAASIEMTSTAEDATKTASNDGRPSATAKKGEVIPMTVTVRDSAGNPLPGASFNLK
                     RGTALNRAKAAYDASADDLTIIPVEPTGVTSILYGDGTQALLKTGSDGKATFEVSQNS
                     SYGLSTPLSAELMRDTSKSVTLDVIFTVITSPDSPKAKYWGHMPETFTSSAGVTFKRP
                     LLAAEATTGSSVNGNNETWSYIYSTQKATADCSLEYQPRLSELQGLYDDHPNGALTKD
                     LGLPIASGNWWIYELLNSNGSSWYYQVFNLSTGRVSSALSPVALMLCLAQPHSKPSSV
                     TLTSIAFDETRTASNGGTPSASAKKGETIPLVVTVKDQNGNLVSGEGVTLQRAQAKSR
                     SGIRPSSLADDLIVDVVTPTATRISFAQDTAKWLGFTGSDGTVTFNVTQNATTGLATP
                     FTVSLAQDPQITSNLDLIFTVVTSPDAQDADYWGHMPETVTAANGTVFERPKLWKELP
                     STSGVNKTNNNNEDWPYFSSTTKANASLSPCEEARQPLLSDMKSLYQRYPNNTLTTET
                     GWSVYYYWWAQDKTTDGKNQSLNMKDGTTTLNGNAAYQACLVSARATVSSVTLTSTAF
                     DADSQAAKVKKGEAMPVTVTVKDSAGNTVPNVEFTLKRGDASPRNAGATLYGDVVAMD
                     DLIVQPLSGSAVTLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYASAT
                     DTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPIINY
                     ENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSSQQC
                     GMDEGTGAQDCSITLMNKPGLVTCFQ"
     gene            complement(23587..24564)
                     /locus_tag="ROI21_003060"
     CDS             complement(23587..24564)
                     /locus_tag="ROI21_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5111955.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEESAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADHVTLTSDLKGARPTGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDDDRGRYIGIEITPTT
                     QTGTPNVGTALHLYDISTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLAIPATNDEARTNLNGPARDGKEALTIPTNGDGVQGYKLHIIYKHK"
     gene            complement(24619..26796)
                     /gene="sinH"
                     /locus_tag="ROI21_003061"
     CDS             complement(24619..26796)
                     /gene="sinH"
                     /locus_tag="ROI21_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HDX5111956.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQDGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRHN
                     FNEWLLGGNIFYDYDFTRGHRRLGLGGEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKVVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGADYKAGTGDNSDVSINATLNYQFGVPLKDQLDSDKVKAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPQLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDINKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         6485 a         6946 c         6670 g         6813 t
ORIGIN      
        1 ttatttcgta ccaaagattt tgtcaccggc atcgccgagg cccggaataa tgtatccgtg
       61 ctcgttcagt ccctggtcaa tcgatgcagt atacagttcg acgtccgggt gcgctttttc
      121 cagcgctgcg ataccttctg gcgcagcgac cagcaccaga actttgatgc tgctgcagcc
      181 cgcttttttc agcaggtcga tggtcgcgat aacggaacca ccggttgcca gcattgggtc
      241 aacgatcagc gccatacgct catcgatgtt agaaaccagt ttctggaagt acggtaccgg
      301 ctccagcgtt tcttcattac ggtacatacc gacgacgctg atgcgcgcgc tcggaacgtt
      361 ttccagcaca ccgtccatca tccccagacc cgcacgcaga attggcacaa cggtaatttt
      421 cttacctttg atctggtcga tttctaccgg gccgttccag ccttcgatag ttactttttc
      481 cgtttcgagg tcggcggtcg cttcgtaagt cagcaggcta cccacttcgg aagcgagttc
      541 gcgaaagcgc ttggtgctga tatcttgctc acgcatcagt cccagcttgt gtttgacgag
      601 tgggtgtttg acttccacga tcttcatact ctttctcctt tgaggggcag ccacaaaaaa
      661 aatcgacgga ttatacctcc tttcttcaag gcggcaatat tattttcgtt gactttagtc
      721 aaaatgataa cggtttgaga taaagttatt ttatattcaa atggttatgg aagaagatta
      781 ttccatccga aaacgatcct ttgccctggt acaagtattg ttttgccgcg cgcctgggga
      841 aaagacgtac aaaaaggttg tgtaaagcag tctcgcaaac gtttgctttc cctgttagaa
      901 ttgcgccgaa ttttattttt ctaccgcaag taacgcgtgg ggacccaagc agtgaccgat
      961 aaaacctctc ttagctacaa agatgccggt gttgatattg acgcgggtaa tgctctggtt
     1021 ggaagaatca aaggcgtagt gaagaaaacg cgtcgtccgg aagtgatggg cggtctgggc
     1081 ggcttcggtg cgctgtgtgc attgccgcaa aaatatcgtg aacccgtgct ggtttccggc
     1141 actgacggcg taggtaccaa gctgcgtctg gcgatggact taaaacgtca cgacaccatt
     1201 ggtattgatc tggtcgccat gtgcgttaat gacctggtgg tgcaaggtgc ggaaccgctg
     1261 tttttcctcg actattacgc aaccggaaaa ctggatgtgg ataccgcttc agcggtgatc
     1321 agtggcattg cggaaggttg tctgcaatcg ggctgttcac tggtgggtgg cgaaacggca
     1381 gaaatgccgg ggatgtatca cggtgaagat tacgatgtcg ctggtttctg cgttggcgtg
     1441 gtagaaaaat cagaaatcat cgacggctct aaagtcagcg atggcgatgt gctgattgca
     1501 ctcggctcca gcggtccgca ctcgaacggc tattcgctgg tgcgcaaaat tcttgaagtc
     1561 agcggttgtg atccgcaaac caccgaactt gatggtaagc cattagccga tcatctgctg
     1621 gcaccgaccc gcatttacgt gaagtccgtg ctggagttga ttgaaaaggt cgatgtgcat
     1681 gctattgcgc acctgaccgg cggcggcttc tgggaaaaca ttccgcgcgt attgccagat
     1741 aatactcagg cagtgattga tgaatcatcc tggcagtggc cggaagtgtt caactggctg
     1801 caaacggcag gtaacgttga gcgccatgaa atgtatcgca ccttcaactg cggcgtcggg
     1861 atgattattg ccctgcctgc tccggaagtg gacaaagccc tcgccctgct caatgccaac
     1921 ggtgaaaacg cgtggaaaat cggtatcatc aaagcctctg attccgaaca acgcgtggtt
     1981 atcgaataat gaatattgtg gtgcttattt ccggcaacgg aagtaatttg caggcaatta
     2041 ttgacgcctg caaaaccaac aaaattaaag gcaccgcacg ggcagttttc agcaataagg
     2101 ccgacgcgtt cggccttgaa cgcgcccgcc aggcgggtat tgcaacgcat acgctcatcg
     2161 ccagcgcgtt tgacagtcgt gaagcctatg accgggagtt gattcatgaa atcgacatgt
     2221 acgcacccga tgtggtcgtg ctggctggtt ttatgcgcat tctcagcccg gcgtttgtct
     2281 cccactatgc cgggcgtttg ctgaacattc acccttctct gctgccgaaa tatcccggat
     2341 tacacaccca tcgtcaggcg ctggaaaatg gcgatgaaga gcacggtaca tcggtgcatt
     2401 tcgtcaccga tgaactggac ggtggcccgg ttattttaca ggcgaaagtc ccggtatttg
     2461 ctggtgatac ggaggatgac atcaccgccc gcgtgcaaac ccaggaacac gctatttatc
     2521 cactggtgat tagctggttt gccgatggtc gtctgaaaat gcacgaaaac gccgcgtggc
     2581 tggatggtca acgtctgccg ccgcagggct acgctgccga cgagtaatgc cccgtagtta
     2641 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
     2701 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
     2761 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
     2821 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
     2881 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
     2941 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
     3001 ttccaactct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
     3061 tcaggaattc gacggcctct acaacgagtt gttgctggag atggcgcgca accagatctt
     3121 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
     3181 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg atttagtgca
     3241 gttcctgaaa gatgattaca cctatctggc ggtggaaatt atccgtggcg ataccatccg
     3301 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
     3361 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
     3421 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
     3481 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
     3541 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
     3601 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
     3661 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
     3721 agccaatctg gtgaacaaac cactgccgcg tttacgccac atttggtttg ataaagccca
     3781 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
     3841 tacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
     3901 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
     3961 cgccgcacac aacggtaaga aagtcaccgt ggtggttgag ttacaggcgc gtttcgacga
     4021 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
     4081 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
     4141 agtggtgcgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
     4201 tactgactat tcgttgctga ccgccgatgc gcgcatcacc aacgaagtac ggcgggtgtt
     4261 caactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcgccgca
     4321 aaactcccgc cgcctgttgt atgaaatggt ggaccgcgaa atcgccaacg cgcagcaagg
     4381 gctgcccagc ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
     4441 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gcatgtgttc
     4501 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
     4561 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
     4621 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
     4681 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
     4741 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
     4801 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
     4861 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
     4921 ttggttcaaa cagttttcac atggtcattg cccgtgtggt agatggtgcc atgcagatta
     4981 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga
     5041 gtgaagaggc aatgacgcgc ggtttaaact gtctgtcgct gtttgccgaa cggctgcaag
     5101 ggttttctcc tgccagcgtc tgtatagttg gcacccatac actgcgtcag gcgctgaacg
     5161 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
     5221 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gagaaaggtc
     5281 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
     5341 aaccgattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttacttcc
     5401 ctggcggggt catcaataaa gagaattttc agcgcgctcg tatggcggca gcacaaaaac
     5461 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgtggcaatg ggcgcttccg
     5521 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
     5581 ccccggaacg tctggaaaaa ctggtaaaag aagttttacg tcaccgtaat ttcgcatcgc
     5641 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
     5701 tatgcggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
     5761 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
     5821 ccgccagcag cctcgccaac cagtatcaca tcgacagtga acaggcccga cgggtgctgg
     5881 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
     5941 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
     6001 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagcgacttg ccgggtttta
     6061 atcaggaaca gcagttgatg atggcgacgc tggtgcgcta tcaccgtaaa gcgattaagc
     6121 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgattcagc
     6181 tattgcgcct tggcgtatta ctcaataatc aacgtcaggc aaccaccaca ccgccaacat
     6241 tgacgctgat taccgatgac agtcactgga cactgcgttt tccgcatgac tggtttagtc
     6301 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
     6361 gctggcggtt gaaaattgaa gaagaaagta caccagaaat cgccgcttaa tgctttgcgg
     6421 gccgacaaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
     6481 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
     6541 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
     6601 ttttcttcat tcgcgccagg tggcaaatcg acgccactat ctgataatcc agactatttg
     6661 acacaatatt gcggataaaa ctgccgtcaa ttttaagcag atcggcattc acatttttaa
     6721 gccgcgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
     6781 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
     6841 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcagc tgactgactt
     6901 caacgggaaa acgagcctga cataccgagg ttggcgacag attaatggca aaacggtgag
     6961 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgttcaatg acccacatgt
     7021 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctgtcg gggctgatca
     7081 gttcatcatt ctcacctttc atacgcagaa gaatttcatg gtagacatcg ccacgcatac
     7141 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagccgat
     7201 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg cgacgctgca
     7261 tattttcagg ggcgttggtc acgatggaaa gctcggcgac cgtatttagc tctcccagca
     7321 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
     7381 gcatcggcat tccatcccaa aagaaacgaa attgcttgag atggctatcc agtgcggtaa
     7441 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
     7501 aaacatcctc acccggttcc agcaagggtg acagccagtg agaaagtttt tgcttgtatt
     7561 gaatccgcag catgatgcca tagttcttaa ccagcatttc catgccaggg atgcgtaaat
     7621 aacaaagcgc agaccagggg gcatcacgca acgcgcgatt cagggcgcga acatttggca
     7681 gatgaaccac cgggtccaca tacgcaagcc gctgaatgcg tctggctacc gctctctgac
     7741 gggttgccag tacagccata taattgacaa taaaagagaa taccagataa ctggaggagg
     7801 ttatggtcag ctgcgtggta tagccaggat aaatgggaat gtaattttga tagctgtgga
     7861 tgctgatcat caacacgacc gcccagagca gcgaaattag cttataacca tagcgcatcg
     7921 ctccccacat catcaggggc agcaataatg acaaggtata gttggtgcta aaaattgtgc
     7981 ttttttcatt taacggcatg cataacagca gcagtaaagc acctaatgcc agtagccaga
     8041 gtgcgaactc ttttttggtg acttttacat caacctgctg ttttaattgc gaataataac
     8101 tacgtaaata aaacggattt cgcactgcac gaatgatgaa gtagcacagc gggacaccaa
     8161 tcagattgcc caccagtaag gcctgataat tgattaaggt cccgaggtta aaaggcatga
     8221 cgccgaccag attttctctg ctcgccagta atcctacaaa cgcagcaaac tggaaaagta
     8281 tcagaaacag cgttgcagga aacacaatct gccagaatat acgctgggaa attaaacggg
     8341 tatcaccatg tgaaacgttg ttacgccggg gagtaaagac cctgtaaccg ccccaacaga
     8401 gaatgataat gataataaaa ttagccgtta atgagagcgt ttcgtaaaaa cccagctctg
     8461 catatttgcg gacaaaaatc cctaaggcga tccctggcaa tgccgcccag ctgaaaaaca
     8521 tcatcatgct gatcatcaga gccagaggca gataaaagag aaaaacctca ccggaagaaa
     8581 tatgcgcgaa agtattaatg tgggcgaaaa tgggcaagat taaagaaggc aggaacagcg
     8641 gaagccccca ccatttatca cgtattttta tataagttgc attcagtttc atagatgctc
     8701 agcagaatcc cccacatcct gaaggaggtg tattcagaca ggcatcccac ctgacttcga
     8761 atgatgatta ttcatcacta tagagagcat tgattctaag tgtcatatga aagttccaat
     8821 tgatatatat caaacaaaat aaccctgatt aatgaattat tacgtttatc atgttaattc
     8881 atcattatta catcatcatt gtaaataatt aaattaactt ccataacatt aaaatatgta
     8941 tccactgacg cttttttaca taacgaagaa ttgaccattt tgtcctgttg tgccttaatg
     9001 taagtaccgt ccacagcgtg ggacatactt caaggaacct tttgtgagtc aggcaaccag
     9061 tatgcgaaaa cgacaccgat ttaacagtcg catgacccgt atcgtactgc tcatcagctt
     9121 tatcttcttc tttggccgtt ttatctactc gtccgtcggt gcatggcagc accatcagag
     9181 caaaaaagaa gctcagcaat ccacactctc cgtcgaatca ccggtacaac gttagcggtt
     9241 accttctcca ctttcacaga acataacggc acctcgctgt cggatgcttt tgctctttgg
     9301 gattatcaaa gcggcagata ttctttcatc ttaaatttta cgtctttgtc ctgactgatg
     9361 tttatcctgt ttggctgcga aataaatata aaattaatac atatgtttta atgatgtatt
     9421 tttataaatt attttccaca tgaattttaa taaatttaat ctattcctta atacgcaata
     9481 catttacttt cctcttttga tgatcttaaa tgtcttattt ttcgtaatgt gtataacaag
     9541 gaatagtgat gaaatttaaa aaatgtcttc tgcctgtggt aatgttagcg tcattcactc
     9601 tggcaggatg ccagtcaaat gctgatgatc atgccgccga tgtttatcaa accgatcaac
     9661 tgaataccaa acaagaaact aaaaccgtta atattatttc cattcttccc gcaaaagttg
     9721 ccgtagacaa ctcccaaaat aaacgaaacg cacaagcctt cggcgcgctt attggtgcag
     9781 tcgctggcgg tgttattggc cacaacgtcg ggtctggcag caattccgga acgacggcag
     9841 gtgcagttgg cggcggagct gttggcgcgg cagcgggttc tatggtgaat gataaaacct
     9901 tagtggaagg tgtttctcta acgtataagg aaggcaccaa agtgtatacc tccacccagg
     9961 tgggtaaaga gtgccagttt acgacaggtt tagccgttgt tattaccacg acgtataacg
    10021 aaacgcgtat tcagccaaat accaaatgtc ctgaaaagag ctaataatca ggaggagtca
    10081 tgaagaaagt ttttctttgc gccatcttag cttccttaag ctatccggct atcgcctcat
    10141 cattgcagga tcaactctct gctgtcgcag aagcggaaca gcaaggtaaa aatgaagagc
    10201 aaaggcagca tgacgaatgg gtcgcggagc gcaacaggga aatccagcaa gagaagcaac
    10261 gtcgcgcaaa cgcccaggcc gccgctaaca aaagagcgac aacggcagcg gcaaataaga
    10321 aagctcgtca ggataaactg gacgccgaag cctctgcgga caaaaaacgc gatcaaagtt
    10381 atcaagatga gctacgcagc ttagagattc agaaacaaaa actggcgctg gcgaaagaag
    10441 aagcccgcgt taagcgagaa aacgaattta tcgatcagga actgaagcac aaagctgcgc
    10501 aaaccgatgt ggtgcaatct gaagctgacg caaacaggaa tatgactgag ggcggtcgag
    10561 atctgatgaa aagcgtgggc aaagcagaag agaacaaatc ggacagctgg tttaattaat
    10621 cgatgtaagt aacttcaagc ctataattct tgaagataaa aaaccctctg tagtaacaga
    10681 gggttttgtt cattcatagt gcagggatca aaatcattcc cactcaatgg tagctggcgg
    10741 cttgccgctg atgtcataca ccacgcggga aataccgttc acttcattga taatgcggtt
    10801 ggaaacgcga ccgaggaaat cgtacggcag atgcgcccag tgtgcggtca taaagtcgat
    10861 ggtttcgaca gcacgcagag agacaaccca gtcatactta cgaccatcgc ccattacgcc
    10921 aacggaacgt actggcagga agacggtgaa cgcctggctg actttgtcgt acaggtccgc
    10981 tttacgcagt tcttcaatga agatggcgtc agcgcggcgc agcaggtcac agtactcttt
    11041 cttcacttca cccagaacac gaacgccaag gcctggtccc gggaacggat gacggtacag
    11101 catgtcgtac ggcaggccca gctccagacc aatcttacgc acttcgtctt tgaacagctc
    11161 tttcagcggt tcaaccaggc ccatcttcat ctctttcggc aggccgccca cgttgtggtg
    11221 agatttgatg acgtgtgctt taccggttgc agacgccgca gattcgataa cgtcagggta
    11281 gatagtaccc tgcgccagcc acttcacgtc ttccagtttc agcgcttctt catcgaatac
    11341 ttcaacgaaa acgcgcccga tgattttacg ttttgcttcc ggatcgtttt cgccagccag
    11401 cgctgacagg aagcgatctt ctgccggaac gtgaacaata ttgagaccaa agtgatcgcc
    11461 aaacatatcc agaacctgct ctgcttcgtt gagacgcagc aggccgttgt cgacgaatac
    11521 gcaagtcagg tttttaccga tagcgcggtg cagcagcatt gcggttacgg aggaatccac
    11581 gccaccagag aggccgagga tcactttatc gtcgcctacc tgctcgcgga tgcgagctac
    11641 ggcgtcgtcg ataattttcg ctggcgtcca cagggcttca cactggcaga tatcacgcac
    11701 aaaacgctcc agcatgcgca taccctggcg ggtgtgagtc acttccggat ggaactgtac
    11761 accatagaag cgtttttctt cgttagccat aatggcaaac gggcagcttt cggtgctggc
    11821 tacggtgacg aagtcggacg ggatagcggt aactttatcg ccgtggctca tccagacatc
    11881 gagcagcggt ttaccgtctg cagtcagcgc atcttcgata ccgcgaacca gtgcgctgtc
    11941 gtttacgact tcaacctgcg cgtagccaaa ttcacgttcg ttagaggctt caacgtgacc
    12001 gcccaactgc attgccatgg tctgcatgcc atagcaaacg ccgaataccg gtacgcctgc
    12061 ttcaaagaca tactgcggcg cacgcgggct gttttcttcg gtggtgcttt ccgggccgcc
    12121 ggaaagaata atgccgcttg ggttgaagtc acgaatttgt gcttctgtca catcccacgc
    12181 ccacagttcg cagtaaacac ccagttcacg cacgcggcgc gcaaccagtt gagtgtactg
    12241 agaaccgaag tccagaatga ggatgcgatg cttatgaatg ttttccgtca ttgacgctta
    12301 ttccgaggca agtgaaacag ataatataaa tcgcccgaca tgaagtcggg cgaagagaat
    12361 caggagccca gacggtagtt cggggactct ttagtaatgg tcacgtcgtg aacgtggctt
    12421 tcctgaatgc ccgcaccgct gatacgtaca aactccgctt tagtacgcag ttcgtcgata
    12481 gtaccacagc cggtcaggcc catacaggag cgcaggccgc ccatctgctg gtgaatgatc
    12541 tctttcaggc gacctttata ggccacgcga ccttcgatac cttccggcac cagtttgtcg
    12601 gcagcgttgt cgctctggaa gtaacggtca gaagaacctt tggacatcgc acccagagag
    12661 cccatcccac ggtaagattt gtaagaacgg ccctggtaga gttcgatttc acccggagat
    12721 tcttcagtac ccgccagcat ggaacccacc attaccgcgc ttgcacctgc ggcgatagct
    12781 ttggcgatat cgcctgagaa acggatacca ccgtcagcga taaccggaat accggtgcct
    12841 tccagggctt ctacggcgtc agcaacggcg gtgatctgcg gaacacctac gccagtcacg
    12901 atacgagtcg tacagataga gccagggcca atgccgactt taaccgcgct gcaaccagct
    12961 tctgccagag cgcgtgcccc cgcagctgtt gccacgttgc cgccgatgat ttgcagatcc
    13021 ggatatttag cacgagtttc acggatacgc tgcagaacgc cttcagagtg accgtgagag
    13081 gagtcgatca gcagaacgtc aacgcctgcg gcaaccagcg cgtcaacacg ctcttcgtta
    13141 cccgcacccg cgccaaccgc tgcaccaaca cgcagacggc cttgctcgtc tttacaggcg
    13201 ttcggtttac gttctgcttt ctggaaatct ttcacggtga tcatgccgat caggtggaat
    13261 tcgtcatcaa ccaccagcgc tttttcaacg cgtttttcgt gcatttttgc cagcacaact
    13321 tcacgggctt caccttcacg cacggtgacc agacgttctt tcggcgtcat gtaaacgcta
    13381 accggctggc tcaggtcggt aacaaaacgt acgtcacgac cggtgataat acccaccagt
    13441 tcgttttctt cggtaacgac cgggtagccc gcaaagccgt tacgctcggt cagttctttc
    13501 acttcgcgca gcgtcgtggt tggcagaaca gtctgcgggt cagtcaccac gccagattcg
    13561 tgttttttca cacggcgaac ttcttccgcc tggcgttcaa tggacatatt tttgtggata
    13621 aagccgatac cgccttcctg agccagagca atagccaggc gcgcttccgt tacggtatcc
    13681 attgctgcgg aaagcatagg gatattcaga cgaatagttt tcgtcagctg ggtgctgagg
    13741 tcagcagtat tcggcagaac ggtagaatga gcaggaacga ggagaacgtc gtcaaacgtc
    13801 agagcttctt tagcgatacg tagcatgggc aatatctcga ccagagtggt taataaatat
    13861 tgccgcggca ttatacagag cgtaaccgat tgcatctacc cctttttgca aaaaatgctt
    13921 gctatccccg gagggcgggt tactatcgac tgaataacct gctgatttag aatttgatct
    13981 cgctcacatg ttaccttctc aatcccctgc aatttttacc gttagtcgcc tgaatcaaac
    14041 ggttcgtctg ctgcttgagc atgagatggg acaggtttgg atcagcggcg aaatttctaa
    14101 tttcacacaa ccggcttccg gtcactggta ctttacgctt aaagacgata ccgcgcaggt
    14161 gcgctgtgcg atgttccgta acagcaatcg acgggtgacc tttcgcccac agcatgggca
    14221 acaggtttta gttcgcgcca atattacgct ctacgagccg cgcggtgact accagattat
    14281 cgtcgaaagt atgcagcctg ccggtgaagg gttgctgcaa caaaaatacg aacagcttaa
    14341 aacgaaattg caggctgaag gtctgttcga tcagcaatac aaaaaaccac ttccctcccc
    14401 tgcgcattgc gttggtgtga tcacctcaaa aaccggtgct gcgctacatg atattttgca
    14461 tgtgttgaaa cgtcgcgatc cttctctgcc ggtgatcatc ttccccaccg ccgttcaggg
    14521 cgatgacgcg ccggggcaaa ttgttcgcgc cattgaaatg gcgaatcagc gcaacgagtg
    14581 tgacgtattg atcgttgggc gcggtggcgg ttcgctggaa gatttatgga gttttaacga
    14641 cgaacgtgta gcgcgggcga tttttgccag ccgcattccg gtagtgagtg ccgtcgggca
    14701 tgaaaccgat gtgacgattg ccgattttgt cgctgatttg cgtgcgccaa cgccctcggc
    14761 tgccgctgaa gtagtgagcc gaaatcagca agagttactg cgccaggtgc aatcggcccg
    14821 tcaacggctg gagatggcga tggattatta tctcgccaac cgcacgcgtc gttttacgca
    14881 gatccatcat cgcttacagc agcagcatcc acagctccgg ctggcacgcc agcaaaccat
    14941 gcttgagcgc ctgcaaaagc ggatgagctt tgcgctggaa aatcagctta agcgtgccgg
    15001 gcaacagcag cagcgattaa cacagaggct aaaccagcaa aatccgcagc cgcgtattca
    15061 ccgcgcacag acgcgaattc aacaactgga atatcgcctg gcagaaacgt tgcgcgcgca
    15121 actgagcgca acgcgagaac ggtttggtaa tgcagtaacg cacctggaag cagttagccc
    15181 gctttcgacg ctggcgcgtg gttatagcgt caccagcgcc gcagatggcg cagtgttaaa
    15241 gcaggtgaag caggtgaaag cgggcgatac gttgaccact cgacttggtg atggtgtggt
    15301 gattagtgaa gttagcgccg taacaaaaac gcgtaaaccg cgtaagaaag ctgctaatcc
    15361 ataagatgcg ccagcatcgc atccggcact ggctcccagg ccacgccaga tgcgccatgc
    15421 tggttactgg aagcaggtca ccagacccgg tttattcatt agcgtgatac tacaatcctg
    15481 tgctccagtg ccttcgtcca tcccacactg ttgagaggac aaataaggaa aacttggtga
    15541 agacggccat cccagtgaat taaaaacagt gtgcaacaac tctaattcat tgtatgtcgg
    15601 tgcattttct atgctgccac actgccttgc aatatcccat ttactggcat caatgatatg
    15661 tgcagaagcc cagttttcgt agttaataat cggtgaggat acgggatttg ccccggacgg
    15721 caattctgtc gttaacaacg gtcgatgtag cgatttacca ttcaccacta cagtgtctgc
    15781 catatgtccc caaaagtgcg ccgaggaaac attcgggctg gttggtaccg taaaaatagc
    15841 gtccagcgtg tctgtcgcgg aagcgtaatt agccagagta accgtcagtg gcgttttata
    15901 accaggggta ttatcctggc ggagcgtaaa acttgccgtc ccatccgcgc ctgtcatgcc
    15961 actaatggta ttaccgcttt cactcagcgt aacagccgaa ccagataacg gctgaacgat
    16021 gagatcgtcc atcgccacca catcaccata caacgtcgcc ccagcgttac gcggcgatgc
    16081 atcgccacgt ttcaaagtaa attccacatt cggtaccgta ttcccggcgc tgtctttcac
    16141 cgtcacggta acgggcatgg cttcgccttt tttcactttc gccgcctggc tgtcagcatc
    16201 aaacgctgtt gaggtcagtg tgacgctgga aaccgtcgcg cgcgcagaca ccagacacgc
    16261 ctgataagcg gcattaccat tcaatgtcgt tgtgccatct ttcatattga gcgactgatt
    16321 tttaccatct gtagtcttat cctgcgccca ccagtaataa taaactgacc agccagtttc
    16381 cgtcgtaagg gtgttattgg gataacgttg atacagtgac ttcatatcgc tgagcaacgg
    16441 ttgccgggct tcctcacaag ggctgaggct ggcatttgct ttagtggttg acgaaaaata
    16501 gggccagtcc tcattattgt tattggtttt atttaccccg gaggtcgatg gcagctcctt
    16561 ccacaatttc ggtcgttcaa agacggttcc atttgccgct gtcaccgttt ctggcatatg
    16621 accccagtag tcggcatcct gggcgtcagg gctggtgacc acggtaaata tcaggtccaa
    16681 gtttgacgtg atctgcggat cttgtgcaag cgagaccgta aacggcgttg ccaggccggt
    16741 ggtggcgttt tgcgtaacat tgaacgtcac tgtgccatca ctgccggtaa aaccaagcca
    16801 ttttgccgta tcctgagcaa aggaaatacg ggttgctgtc ggtgtgacaa catctacaat
    16861 aagatcatct gccaacgatg agggcctaat tcctgaacga cttttcgcct gcgcccgctg
    16921 aagcgtcacg ccctcaccac tcaccaggtt gccattttgg tcttttactg tcacgactaa
    16981 cgggattgtc tcgccctttt tcgccgatgc cgaaggtgtg ccgccgttac ttgcggttct
    17041 cgtctcatca aatgctattg aagtcagggt aacgcttgac ggcttagagt gaggctgtgc
    17101 caggcagagc attagagcaa ccggagagag tgcggatgat actcgtcccg tacttaggtt
    17161 aaagacctga taataccagg agctaccatt ggagttaagc aattcataaa tccaccagtt
    17221 tcctgacgct atcggcaacc ccagatcttt cgttaacgcc ccgtttgggt gatcgtcata
    17281 taacccctgc aattcgctga ggcgcggttg atattccagc gaacaatctg ccgtcgcttt
    17341 ttgagtacta tatatatatg accatgtctc attattaccg tttaccgagc tccccgtagt
    17401 cgcttccgct gccagtaatg ggcgcttgaa ggtcacgcct gccgaactgg taaacgtttc
    17461 cggcatatgg ccccagtatt tcgcttttgg cgaatccggg ctggttatca ccgtgaaaat
    17521 gacatccagc gtgacgcttt tcgaggtatc gcgcatgagt tctgcgctga gcggcgttga
    17581 cagcccataa ctgctattct ggctgacctc aaacgtagct ttaccatcgc tccctgtctt
    17641 cagaagagcc tgcgtcccat ccccatacaa gattgacgtt accccagtag gttccacagg
    17701 tatgatggta agatcatctg cagaggcatc gtacgcagct ttagcgcggt tcagcgcagt
    17761 tcctcgttta aggttaaagc tggcacctgg cagtggattg cctgcgctat ctcgcaccgt
    17821 aacagtcatc gggataacct ctcccttctt cgctgttgcg ctgggcctgc catcattgct
    17881 ggccgttttc gttgcatcct ccgccgttga ggtcatttca atagaagccg cctctggatg
    17941 agggtttacc aggcacagca ttaatgccga ggtggttgaa gacgctcttc cacttgatcc
    18001 atttaacaga taaatatatt gatcgtacca gttacccgca tatgccacca tgtcgtaagc
    18061 ccaccagtag ctatttaccg gaatacccag atcggtcttt atcgcacgac ttgggtgttc
    18121 gtcgtacaat gcctgtagtt cgcttaacag tggttgataa tgtacatcgc acccgctttt
    18181 atcgatatcc ccctttgttg ccgatctcaa cacggcccac tcttcattgt cttctataaa
    18241 cgactggccc gacgatcttt cagcagagag taaggggcgt ttgaaggcag taccagaacg
    18301 cgtagtgaac gtttccgcca tatgccccca gtaacttgcc aggggggtat ccgggctggt
    18361 tatcacggta aacatcacgg gtaatttgtt ggattccagg ctcgactgat aggcctgagc
    18421 gtacacattg gttaccaggc ctatagaatc atcctgcttt accgtaaacg tggctgtccc
    18481 gtctgcacct gtcaccgcat aaagggagat tgatggagta tccactcttg ccgacgttcc
    18541 cgcagcgttg attacagtga tagttctgtt cgacaactgt acattctggc gatttgtcga
    18601 tccttcacgt ttgagaataa acggaacatt tggtacgaca tttccctgcg catcttttgt
    18661 caccacgcga atcaccgcgt cgtcaccttt tttccgtttt agtgcgctga gggtttcgtc
    18721 aactttgaca aattgtgtcg catcgttcgc ttcaacaata atgtggctgg tcaccgccac
    18781 tggggtagcc cgacaactta gcagtcctag actgcttgac atgacctccg ttccatcacc
    18841 cgtttttaac gaaaccgctt taaaattaaa tgtttgagtg taataggcaa caaccgtact
    18901 ggagatatag gcgcgtgata caggccatcc cgtaacgtca tgtattggca cgtaaccttc
    18961 atcggcatac agtgactcca gctcactttc aatcgggaca tatcctgcac cgcaaccatt
    19021 tacagatgtg ttgttcgact ggtccggcga gaacagtgac cagctctctc catcttcctg
    19081 aaaagagctt cgactggttg taacggaaag ctcatcttgc aataacggtc ttttataaat
    19141 aagcccgcct gcaccggtca tcgtttcggc catatgcccc cagaattttg ctttaggggt
    19201 atcggggctg gttatcaccg taaagaccac cggaagcatt gattttacgt tattatctgt
    19261 atccagcatg gcggtcagtt cggtttttaa acccgtggta ttgtcctgtt tcagtgcaag
    19321 tgttgtcgta ccatctgcgc cggtaacgcc ataaatcagt gctgttgttg aggaaaaatt
    19381 actctgcgtg ttaccccagg catccgtcac gatgagcgcg cctgctgtca ccgttgtaga
    19441 cgcatttgcc cgattgacgc taaggttacg tttaagcgta aacgcggtat ttcccaccgg
    19501 gttaccctgc gaatctttag tggtcacgcg aaccacgact tcttcccctt ttttcgcttt
    19561 gacaacatta ttcgtactgt caaattttgt ggggtccgca gcctcaagaa cgatttggct
    19621 agccttgacg atcggcgtcg tctggcaggt aagcagttct tttgtggccg atggaattat
    19681 cgaactggtg cctgaactga gattgacggc cttgaagtca cgctgttcca ggctactgct
    19741 gcctgtcgtg ctggaaaggt aacttgaggc gacgggccag ccctgaaccg ttttcatcgc
    19801 attgccgcgg ttggcttcat acaatgactc cagcccggcc tgggagggaa catattcact
    19861 accacaacca ttagaggatg tgcttgtcgc ctgagtttgg gtaaattgcg cccagtcctc
    19921 attatcttct gcaatcgctg ttcgacccga cgtactggat aactctttca acaataatgg
    19981 gcgtttaaag accgttccat ccacagccgt caccgtttca ggcatatgcc cccacatttt
    20041 cgccttatcg ctgtccgggc tggtgacaac ggtaaaaatg gtatcgaggg tcgctttttt
    20101 cgttgtgtct gaatagagcg cggcggtcag cgacgttttt gtgccatggg tatccgggcg
    20161 ggtaatagtg agaattttag tgccatcgct gccggtcatc gcgctgtatg ctgcggcggt
    20221 atcattgagc gaagtttcat ccgccaggcc actattgatc acaaccggcg caaccagcgc
    20281 atcaccgcta ccggcaatgt gtttttcgtt tgatcgggta taaccatcgc cacgcttcag
    20341 cgtaaaaggc atatcaccca atggattgcc ctgagcatct tttaccgtca ccttcacctg
    20401 tagcgtttcg ccttttttca gcttcgccgc atttgctgcc gcgttccact gcgccgggtc
    20461 caccacttcc agcgtaatgc tgctggcagg attgttagcc gtcgtcaggc aactgacata
    20521 aaccgccgtg ctaccaccaa cggtccgcgc gccatcgttg agggctatgg tgtagtaatg
    20581 cggcacctga tccgcgggcg aactgctcca gtagggctga cgctgcgttg gccaaccgtg
    20641 taccgtttga acggcgttac cactgttagc atcatagagc gcgttaagct gggaacggcg
    20701 tgggagcata ttggcaccac agccaccttt actggtgctg gaagtgttgg cctggctaac
    20761 gcgcgaccat acttcgttat tttccgttaa tgtgccgtcc gggctggaca cttccgcagc
    20821 cagtcggggt cgggcaaagg ttaatgcatc gacggtaatc gtctcatcca tatgccccca
    20881 catctgcgcg ccaaccgcat ccggactggt gagcgtagtg aaaatcacat tgtaattaac
    20941 agtattggga atataggagt ttacaggcgc aatattcagc ggcgtcgcta agcccacacc
    21001 ttgcggttgc ttaatttcca cagtagcaaa accctgcgca tccgtagtac ctgcatagac
    21061 taaagacggt tgcgatgttc cgtagagcgt gccattcatc tcgattgcgc cgcgggttgg
    21121 atcatcgaaa cccgtggtct gcccctgacg atttttcccc atatctttcg tgatggtgaa
    21181 cgcggtatac ggcacgggcg cattattcaa ggcattaaag gtgcgtaccg tcatggtaat
    21241 cgtttcaccc actttcgcct gcgctctgct gcttgtggaa acatcatgat cggtggtcgc
    21301 agcaatctgg gcgaccatct cattgcccga acaagaaagg tagttttgct taaagccgga
    21361 ataggtatcg atgttgcccg tggctaggtt caccgacgca tgcgacgtct cttccaccgc
    21421 actaagatac gctttttgca acgttggcca gccatattcg gtgccgatga cattggctgg
    21481 atgtgcggca aacaaactgt gcaatgagct ttggctcgga atatgaccta aaccacactc
    21541 agcctgcata ctgctattct gatcaaacag cgcccagtct tcgttatttt cccgcactgc
    21601 tcccagttcg ttggcggttt catccgccag ccgtgggcgt ttaaagatat tgttcgcttc
    21661 aataatgcct aacatatgtc cccacatccg cgctttgtcg gtatcggggc tggtgatcac
    21721 cgtaaaaata acgtctttct catccgaggc agtaaaatca ctgcgcattc tcgcggtgat
    21781 gtgagttttc acccccacgc cgcctggctg ggtcagcgtc agagaggcct ctccgttagc
    21841 atcagtgatc ccttcatagg tatgggcgtc aactttgcga acgttactgc cgccatcaat
    21901 ttgtaccggg tacgcctccc acgcggcgtc agtttgattc ttacggttaa cgccgtcatc
    21961 cagatgcagc gaaaagtagt agtacgccag cggctgatcg ttgttcttgc tatcggtaat
    22021 cgtcaggcgt aaagaagcct cttcccccac ctttactttc atcgctttta tcgtcgaatc
    22081 atagctgccc ggcgtcagta ccagcttcgg ttcaacatcg ggcgcttctt tatcgacaca
    22141 gctcacaata aacgtcgtat cgtccggttt ttgcgcttcg ctgcggttcg aaacatccgc
    22201 cgcatggtgc tggccgctca tccccgccag tgatgaccag tagtagttac cctgcatcgg
    22261 ccaaccagcc acatcctgca ctgacgttgg caccaccgtc gccagcgcac cgaactgacg
    22321 cattccgggc aaaatgtcgc aatgtttatc cgcgccactc caggtgaagg ttgaccaggt
    22381 ttcattgtga tcgttaaccg taccgtcttc attatcgact tccgccgcca gttttggtcg
    22441 gctaaaggtg taatcaaggg ccttcagcgt atcgggcata tgaccccaca ttgtggcctg
    22501 cgggacatcc gggctggtga ggacggtaaa gatcaccgcc gtttcactgg cctgagcaat
    22561 acccgcgagg ctcgcgacca acggcgtttt tacaccaggg ccgttggcct gggtgaccgt
    22621 aaccgtggca acaccattcg catcggtaga accacgatat tccgtcgcgg tggtggttaa
    22681 ttcggtcgtg cccagtttta ccggcgcagt attgttcacc acacccaagc ggttttcagc
    22741 atctttgcgt ttgataacaa aaggaatatt ccccacaaca ttaccgacgc aatctttggt
    22801 agtgaccgtt aacgtgatac tgccgccaac cgtcgttttc gccgcaccgg aggattcgtc
    22861 atagcccgcg gcgctgacgg aaataaggtc accctgcgcg ggcgcaatct tcagcgtcac
    22921 tttctgatcg gcgagcgtca gaacactttc atagggatag cccgtcgagg tgagtaaata
    22981 taacagcggc atggtctgtt ggggatcagg ctgcgcccag gcgaccagcg aacgcccggc
    23041 aagtccctga gcgcaaaacg agcgatctgc gactggctgt gggtccagta aggtgtccgg
    23101 cgtcgcggca tccgcccaaa ctaaagatac tgtcggtggc gtttggtttt cccaacgtgg
    23161 ggtggaaatg atatcgcctt ctctgttttg cggattggtg acgagcaatt tagagacgct
    23221 aatatccgcg ctgaattcac tcggtttagc ggtaaacgct acctcatgcg tctttgcagg
    23281 atccagaaag acactgccct ggtagacggg tatgctggca ctgtcggcgg tgggcacggt
    23341 accgttaaat tcgccgaccg tatctgaaga gtcttgccat gcagattctg cccatgacca
    23401 cccaggcagc attgcgctta tcagcaatgc gcataacaca aggattttat tcagggtcag
    23461 ggacatgaca gaaactccac cggttgttaa acggacgctt gtcgcatcaa catattttgc
    23521 ttaatccggg attaagcaaa atatgcggaa acagcattcc cttgcgggaa tgctgctgag
    23581 tacgcgttat ttgtgtttgt aaataatgtg aagtttgtaa ccctgtaccc cgtcgccgtt
    23641 tgtcggaatg gtaagagcct ctttaccatc gcgcgcagga ccgttaaggt tggttctggc
    23701 ttcgtcgttg gtcgcaggga tggccagatt gttattatcg aagcttgagt taacgatacc
    23761 acccgaagtg ccgagctgct ggctgctgcc agaaagtacc caacggaagt cataattggc
    23821 ggtaacgtca gcatcggtac ccgcatcaaa cttgccgttt ttgttctcat ccgagtacag
    23881 tttggccacg taggttttgc caagttcgat tggcgtgctg ccgttgataa ggttgatact
    23941 ggtaccatca acaaagatgg cgactttcag gttctggtta accaccggcc ctggcgcaac
    24001 gttatcgctg tcgcttccgc cgccgctggc agtggaaatg tcataaagat gcagcgcagt
    24061 cccgacgttt ggcgtaccgg tctgcgtggt cggcgtaatt tcaataccga tataacgacc
    24121 acggtcgtca tcggtaagag tgtaactggt caccttcgtt gccagctctt tcggatcgcc
    24181 cccctgagcg tccttgtagc tcatccagcg gatagtatcg atagtctttg cgttatcgcc
    24241 caatccccca agatccacgt cgccttcggt atcaccaatt tcccaactta cggttaattt
    24301 atcacccgag taaagacgct tgtccgtttc actgcctgtc gggcgagcgc cttttagatc
    24361 agaagtcaac gttacatggt cggcatctgt cgtagcgctt cccccttccg aatagagcca
    24421 cggaatggtt ccctggaaaa tattggtttc gccgctgtcg acccaggcgc tttcttccgc
    24481 cagcgccaca ggtgccacac aataacctgc cagtgccagc gccaatgcga tccgggtcag
    24541 accgcgtctt ttatcctgct tcattgatgc tctccttaat gaaccttcag cgactggccg
    24601 actggcctta cgccaatctt attttttggt atacagcacg cgtaaaccgt atccctgcaa
    24661 gccatctccc gcttgtgcgc cataggtttg cgctgcttcg cggttagtgg ccggaatcac
    24721 aatgtcctca ttggaagtgt tcgcctgtgc acccacttca ccattgatgc tcttgccggt
    24781 aaattcccac ttgtagtcgt actgcgccat ctcttcgtca gtcagttttt cgacctgctg
    24841 gaagacaccg tctttattga tgtcacgcca caaaacaaat cgataagtgt tgttgagcgg
    24901 cagcggttct ttatctttac ctatcaggtt gctgggttta gccgccccca cacgatagat
    24961 aaatgcattt agcccaccaa tttctgcccc gaggaaagtg acatccacat agttactgtc
    25021 gtcgtagggc gcagcttttg ctttccagcg ataggtgccg ggtaaggtac tggagagggt
    25081 gacagtgccc ttttctttat cttgtttagc gataaaaacg caggggttgt cggtcgagca
    25141 aggcttgttg gttaacgtca ctcgtttgtc ctgcgcgtcg taaagctgcg gcgtgaggtt
    25201 atcttccggc agcgcatcgc cattgcggtc ggtgttatcg ccaaagctgt cggttacatc
    25261 gagatccatc accacaccat caacgccatc tgcctgagcg ctggcttcgt ggctgtggtt
    25321 gttctcttcc ggcacgttcg cgatgttatt aacaatcaac tctattttac ggtcctgctg
    25381 aacggtgatt tccaccacgc cagagttctg tcggttgccc ttttcatctt ccagtgccag
    25441 acgtactcgc caggtattga gtttttcttg ttcggctttg tcagagctgt actgccaggc
    25501 gggcagcacc aggttccagt ggttattgtt accctctgtc agggtattct ctttgataac
    25561 cggcataacc aacgtgcggg cagcattgtt tttcgcctgc catgacgcag cgacaatttt
    25621 gtagtggtgg ttgataagcg cgttaatggt ccacttacat ttttccagcc cgacggccgc
    25681 ttcgggagtg tccttaatga cgcactcagg gtgctcgttg gtggcatccg cttttaacca
    25741 cagggtgaca tcaagcggat ctttttcttt gtattccaga acaataaagt tattacgctc
    25801 aacaaaatca tgacgactgc ccatcaacga gtgagccgct ttcactttat cgctatccaa
    25861 ttgatctttt agcggaacgc cgaactgata attaagagtg gcattaatgc tgacatcgct
    25921 gttatctccg gtccccgctt tatagtccgc cccaacggtt agtaatggca ccggttgata
    25981 attcagtcca agtgttaccg cgtagggatc tttctccaaa ttatccgtac caaaaagggc
    26041 gacttcatcg ccgtaatatt gctcgaagac aactttgccc cccagttgcg gataagaagg
    26101 taaccaggct tcggcacgaa tatcccagcc gcgcgcaggg cgttcttcat aaaagtcgaa
    26161 atcttcagag tctttccagt cagaaagcgg atgataatag ttgccagaga attttaaata
    26221 atcagtccac gcttcgccgc ctaaacctaa acggcgatgt cctcgggtaa aatcataatc
    26281 ataaaatata tttccaccca ataaccattc attaaaatta tgtcttaccc caagaccaat
    26341 attcccgatt gtacgatctt cttttcgctg cgcagaaaat tgactgaaat aaacagtggt
    26401 ttgattatcg taccagggaa caaaataatc gatggaactg ccatccagat cgccgtcctg
    26461 gccaatggag aggttcgaac ggaccttacc gtagggagag agtgtgtctt caatataaga
    26521 ttgtgcggtt gaggtgattt gactttgcag atagcttctg gcctgagatt ccagcgcctc
    26581 aggggtcaag ttttcaaacc cttgcgtggc ggagttgatg acgtaatcgg ctccgcgctc
    26641 tttcagcgat ttacccttgt tggtttgttc atcctgctgt gctgcctggc tacctaaatc
    26701 aggtaacgat tcgttttgct taacatttat ctctggtgcc gcgaaagcag caccagagat
    26761 aaatgttaga ataatacagc gtttccatcg cagcatgcac tttccctgtg acaatgatac
    26821 agtcaaaaat aagctttaat attgtatccc tgattattca gaaaggcatg tcataaagaa
    26881 taaatatatt cttaattatt taaattagtg attt
//
LOCUS       DAPOZO010000067        25913 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPOZO010000067 DAPOZO010000000
VERSION     DAPOZO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.067"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(47..1345)
                     /gene="tig"
                     /locus_tag="ROI21_003062"
     CDS             complement(47..1345)
                     /gene="tig"
                     /locus_tag="ROI21_003062"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDX5111957.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(1689..2006)
                     /gene="bolA"
                     /locus_tag="ROI21_003063"
     CDS             complement(1689..2006)
                     /gene="bolA"
                     /locus_tag="ROI21_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDX5111958.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            2311..2889
                     /locus_tag="ROI21_003064"
     CDS             2311..2889
                     /locus_tag="ROI21_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX5111959.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTMTLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2933..4408
                     /gene="ampG"
                     /locus_tag="ROI21_003065"
     CDS             2933..4408
                     /gene="ampG"
                     /locus_tag="ROI21_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDX5111960.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLICRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            4868..5815
                     /gene="cyoA"
                     /locus_tag="ROI21_003066"
     CDS             4868..5815
                     /gene="cyoA"
                     /locus_tag="ROI21_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDX5111961.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            5837..7828
                     /gene="cyoB"
                     /locus_tag="ROI21_003067"
     CDS             5837..7828
                     /gene="cyoB"
                     /locus_tag="ROI21_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDX5111962.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            7818..8432
                     /locus_tag="ROI21_003068"
     CDS             7818..8432
                     /locus_tag="ROI21_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDX5111963.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            8432..8761
                     /locus_tag="ROI21_003069"
     CDS             8432..8761
                     /locus_tag="ROI21_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDX5111964.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            8773..9663
                     /gene="cyoE"
                     /locus_tag="ROI21_003070"
     CDS             8773..9663
                     /gene="cyoE"
                     /locus_tag="ROI21_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDX5111965.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGRIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            9812..11176
                     /locus_tag="ROI21_003071"
     CDS             9812..11176
                     /locus_tag="ROI21_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5111966.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETAGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(11304..11795)
                     /gene="yajQ"
                     /locus_tag="ROI21_003072"
     CDS             complement(11304..11795)
                     /gene="yajQ"
                     /locus_tag="ROI21_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDX5111967.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            11963..12874
                     /gene="panE"
                     /locus_tag="ROI21_003073"
     CDS             11963..12874
                     /gene="panE"
                     /locus_tag="ROI21_003073"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDX5111968.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATGENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            12837..13427
                     /gene="yajL"
                     /locus_tag="ROI21_003074"
     CDS             12837..13427
                     /gene="yajL"
                     /locus_tag="ROI21_003074"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDX5111969.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARIKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(13481..14929)
                     /gene="thiI"
                     /locus_tag="ROI21_003075"
     CDS             complement(13481..14929)
                     /gene="thiI"
                     /locus_tag="ROI21_003075"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDX5111970.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            15135..15377
                     /gene="xseB"
                     /locus_tag="ROI21_003076"
     CDS             15135..15377
                     /gene="xseB"
                     /locus_tag="ROI21_003076"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDX5111971.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            15377..16276
                     /gene="ispA"
                     /locus_tag="ROI21_003077"
     CDS             15377..16276
                     /gene="ispA"
                     /locus_tag="ROI21_003077"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDX5111972.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            16301..18163
                     /gene="dxs"
                     /locus_tag="ROI21_003078"
     CDS             16301..18163
                     /gene="dxs"
                     /locus_tag="ROI21_003078"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDX5111973.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            18218..19192
                     /gene="yajO"
                     /locus_tag="ROI21_003079"
     CDS             18218..19192
                     /gene="yajO"
                     /locus_tag="ROI21_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDX5111974.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     RGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            19423..20994
                     /locus_tag="ROI21_003080"
     CDS             19423..20994
                     /locus_tag="ROI21_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDX5111975.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKHDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALTPDPNFNIDASSDLDSYLNLKFDNLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKVGENLMIRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPAIDFGIDLGNCNCSNVNFEHTYFDSVKFTNT
                     NCTNANFNSCRFIKCDLTNMNCTGAILDNAVIYGKEKEPEMQYPEADQIIQRITYQKS
                     HGNETKGMILTNCSCVKTTFNWADLSESDCQNVDFSEANLSNTILPDIVRMKGTKLYR
                     TDLFNPILKAEAESTEEKDISPLAKIILDYIESDKNPESLKFDEKSTTTKIIQDIDNF
                     IFYNKHLKSIFNQAMNLEGKISRKKYNEIFNYKQEQARQYFIDQYKLTKNDYLKKIPL
                     TAQLIAKYKMDDQLDQLLVTREIQDEIKPKIQDKIDELSKNLFNTINQTIKDNFDDIF
                     RQQSENMSNYYEFVD"
     gene            complement(21171..21686)
                     /gene="pgpA"
                     /locus_tag="ROI21_003081"
     CDS             complement(21171..21686)
                     /gene="pgpA"
                     /locus_tag="ROI21_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDX5111976.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(21664..22641)
                     /gene="thiL"
                     /locus_tag="ROI21_003082"
     CDS             complement(21664..22641)
                     /gene="thiL"
                     /locus_tag="ROI21_003082"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDX5111977.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRAGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADVKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(22719..23138)
                     /gene="nusB"
                     /locus_tag="ROI21_003083"
     CDS             complement(22719..23138)
                     /gene="nusB"
                     /locus_tag="ROI21_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDX5111978.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(23158..23628)
                     /gene="ribE"
                     /locus_tag="ROI21_003084"
     CDS             complement(23158..23628)
                     /gene="ribE"
                     /locus_tag="ROI21_003084"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDX5111979.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(23717..24820)
                     /gene="ribD"
                     /locus_tag="ROI21_003085"
     CDS             complement(23717..24820)
                     /gene="ribD"
                     /locus_tag="ROI21_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDX5111980.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHH
                     RAGEPHAEVHALRMAGDKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(24824..25273)
                     /gene="nrdR"
                     /locus_tag="ROI21_003086"
     CDS             complement(24824..25273)
                     /gene="nrdR"
                     /locus_tag="ROI21_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDX5111981.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            25424..25552
                     /locus_tag="ROI21_003087"
                     /pseudo
     CDS             25424..25552
                     /locus_tag="ROI21_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            complement(25648..>25913)
                     /locus_tag="ROI21_003088"
     CDS             complement(25648..>25913)
                     /locus_tag="ROI21_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5111982.1"
                     /translation="ILDQGWYEMRRQLEYKQLWRGGQVLAAPPAYTSQRCACCGHTAK
                     ENRLSQSQFRCQVCGYTANADVNGARNILAVGHAVLACGEMVQ"
BASE COUNT         6197 a         6690 c         6476 g         6550 t
ORIGIN      
        1 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
       61 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
      121 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
      181 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
      241 ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca
      301 ggccaacaac tacgcgacgt ttagcctgtt cttcgaacag ttcacgcggc agttccagag
      361 cttgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
      421 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
      481 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
      541 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
      601 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
      661 tacctttcag gttttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
      721 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
      781 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
      841 tgaagtcgat ggttacgcgg tcttctgctt caacagcgcc gtctttttct ttccaggtcg
      901 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
      961 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     1021 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     1081 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     1141 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     1201 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     1261 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     1321 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     1381 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     1441 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     1501 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     1561 cgggcaattt tcgtctgaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     1621 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     1681 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     1741 tgcaaccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     1801 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     1861 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     1921 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     1981 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     2041 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     2101 ggtttcgtcc tggtttttct gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     2161 aggcctcgca tcagcgtttc ttttaccagg ggcgattatt tcgtcaacat gatgaattta
     2221 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     2281 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     2341 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     2401 atgacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
     2461 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
     2521 accgcctcac gcgatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
     2581 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
     2641 tatgctgatg tgtcccaggg caacgtgcgc tataacatcg cgaccaaagc ggatatcgct
     2701 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaga actatcgtgc cagctacaac
     2761 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
     2821 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
     2881 gcgcgttaat ttctgccctc tggcccggtg caagccgggc ctgtagacgc ccatgtccag
     2941 tcaatattta cgtatttttc aacagccacg ttcagccata ttgctgattc tgggttttgc
     3001 ttccgggcta ccgctcgccc tgacatccgg caccttacag gcctggatga cggtcgagaa
     3061 tatcgatctt aaaaccattg gtttcttctc gctggtaggc caggcttacg tttttaaatt
     3121 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
     3181 gctgctcgcc acgcaaattc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
     3241 tggcacccaa ctccgctgga tggcggcact ggcagtggtg atcgcttttt gctctgcctc
     3301 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
     3361 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
     3421 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
     3481 gttgatcccc tgtattatcg cgacgttact tgcaccagaa cccaccgaca ccattcctgt
     3541 gcccaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
     3601 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgctatgag
     3661 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
     3721 taacaaaacg cttggcttac tggcgaccat tgttggcgca ttgtacggtg ggattttgat
     3781 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcatctaa
     3841 cgccggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgctgccgt
     3901 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcgc tgttaatgac
     3961 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttctgctgt
     4021 agggcgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
     4081 attctatctg ttctccgtcg ccgctgccgt accagggctt attttgctgc tgatttgtcg
     4141 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccggc
     4201 aggttatgcc tttgctatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
     4261 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
     4321 ggaagtcggg gttttagttg ccctttctgg cgtcgtgctt ggtggtttac tggattatct
     4381 ggcgctacga aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact
     4441 cgccgtaatt attacggcta aataaacatc agcatcgctt attaatattt gttcttttgt
     4501 gcggcttagc gtctggaaat tatagacgcc atttatgaat tctatttttc ttacacgatt
     4561 cagctaatga gtctttattt tctcactacc cagttgtcac cttaatgata attatttgtt
     4621 aaataattgt tttatttcgc attggttata ccaattgccc gcccagactc cttctgcact
     4681 tcccttttgt tataacgccc ttttgcaaca gcttcttaaa atcaacctga tatgttttgc
     4741 aacatatgtg acctggaagc caaatccaag taacaggaat ttaatcatgt ttacagtaat
     4801 gtaaccttcc cgtaaaatgc ccacacactt taaacgccac cagatcccgt ggaattgagg
     4861 tcgttaaatg agactcagga aatacaataa aagtttggga tggttgtcat tatttgcagg
     4921 cactgtattg ctcagtggct gtaattctgc gctgttagat cccaaaggac agattggtct
     4981 ggagcaacgt tcactgatac tgacggcatt tggcctgatg ttgattgtcg ttattcccgc
     5041 aatcttgatg gctgttggtt tcgcctggaa gtaccgtgcg agcaataaag atgctaagta
     5101 cagcccgaac tggtcacact ccaataaagt ggaagctgtg gtctggacgg tacctatctt
     5161 aatcatcatc ttccttgcgg tactgacctg gaaaaccact cacgctcttg agcctagcaa
     5221 gccgctggca cacgacgaga agcccattac catcgaagtg gtttccatgg actggaaatg
     5281 gttcttcatc tacccggaac agggcattgc taccgtgaat gaaatcgctt tcccggcgaa
     5341 cactccggtg tacttcaaag tgacctccaa ctccgtgatg aactccttct tcattccgcg
     5401 tctgggtagc cagatttatg ccatggccgg tatgcagact cgcctgcatc tgatcgccaa
     5461 cgaacccggt acttatgacg gtatctccgc cagctacagc ggcccgggct tctcaggcat
     5521 gaagttcaaa gctattgcaa caccggatcg cgccgcattc gaccagtggg tcgcaaaagc
     5581 gaaacagtcg ccgaacacca tgtctgacat ggcagcgttc gaaaaactgg ccgcgcctag
     5641 cgaatacaac caggtggaat atttctccaa cgtgaaacca gacttgtttg ctgatgtgat
     5701 taacaagttt atggctcacg gtaagagcat ggacatgacc cagccagaag gtgagcacag
     5761 tgcacacgaa ggtatggaag gcatggacat gagccacgcg gaatccgccc attaaagggg
     5821 ttgaggaaga ataaagatgt tcggaaaatt atcacttgat gcagtcccgt tccatgaacc
     5881 tatcgtcatg gttacgatcg ctggcattat tttgggaggt ctggcgctcg ttggcctgat
     5941 cacttacttc ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg tcgaccataa
     6001 acgcctcggt atcatgtata tcatcgtggc gattgtgatg ttgctacgtg gttttgctga
     6061 cgccattatg atgcgtagcc agcaggccct tgcctcggcg ggcgaagcgg gcttcctgcc
     6121 acctcaccac tacgatcaga tcttcaccgc gcacggcgtg attatgatct tcttcgtggc
     6181 gatgcctttc gttatcggtc tgatgaacct ggtggttccg ctgcagatcg gcgcgcgtga
     6241 cgttgcgttc ccgttcctca acaacctaag cttctggttt accgttgttg gtgtgattct
     6301 ggttaacgtt tctctcggcg tgggcgaatt tgcgcagacc ggctggctgg cctatccacc
     6361 gctatcggga atagagtaca gtccgggagt cggtgtcgat tactggatat ggagtctcca
     6421 gctatccggt ataggtacga cgctaaccgg tatcaacttc ttcgttacca ttctgaagat
     6481 gcgcgcaccg ggcatgacca tgttcaagat gccagtattt acctgggcat cactgtgcgc
     6541 gaacgttctg attattgctt ccttcccaat tctgacggtt accgtcgcgt tgttgaccct
     6601 ggatcgctat ctgggcaccc atttctttac caacgatatg ggtggcaaca tgatgatgta
     6661 catcaacctg atttgggcct ggggccaccc ggaagtttac atcctgatcc tgcctgtatt
     6721 cggtgtgttc tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg gttatacctc
     6781 gctggtatgg gcaaccgtct gtatcaccgt gctgtcgttc atcgtctggc tgcaccactt
     6841 ctttacgatg ggtgcgggcg cgaacgtaaa cgccttcttt ggtatcacca ccatgattat
     6901 cgccatcccg accggggtga agatcttcaa ctggctgttc accatgtatc agggccgcat
     6961 cgtgttccat tctgcgatgc tgtggaccat cggttttatc gtcaccttct cggtgggcgg
     7021 gatgactggc gtgctgctgg cagtaccggg cgcggacttc gttctgcata acagcctgtt
     7081 cctgattgcg cacttccata acgtgatcat cggcggcgtg gtcttcggct gcttcgcagg
     7141 gatgacctac tggtggccta aagcgttcgg tttcaaactg aacgaaacct ggggtaaacg
     7201 cgcgttctgg ttctggatca tcggcttctt cgttgccttt atgccgctgt atgcgctggg
     7261 cttcatgggc atgacccgtc gtttgagcca gcagattgac ccgcagttcc acaccatgct
     7321 gatgattgca gccagcggtg cagtactgat tgcgttgggt attctctgcc tcgttattca
     7381 gatgtacgtt tctattcgcg accgcgacca gaaccgtgac ctgactggcg acccgtgggg
     7441 tggccgtacg ctggagtggg caacctcttc cccgcctccg ttctataact ttgccgttgt
     7501 gccgcacgtt cacgagcgtg atgcattctg ggaaatgaaa gagaaaggcg aagcgtacaa
     7561 aaagcctgac cactatgaag aaattcatat gccgaaaaac agcggtgcag gtatcgtcat
     7621 tgcagctttc tccaccatct tcggtttcgc catgatctgg catatctggt ggctggcgat
     7681 tgttggcttc gcaggcatga tcatcacctg gatcgtgaaa agcttcgacg aggacgtgga
     7741 ttactacgtg ccggtggcag aaatcgaaaa actggaaaac cagcatttcg atgagattac
     7801 taaggcaggg ctgaaaaatg gcaactgata ctttgacgca cgcgactgcc cacgcgcacg
     7861 aacacgggca ccacgatgca ggcggaacca aaatcttcgg attttggatc tacctgatga
     7921 gcgactgcat tctgttctct atcttgtttg ctacctatgc cgttctggtt aacggcaccg
     7981 caggcggccc gacaggtaag gacattttcg aactgccgtt cgttctggtt gaaactttct
     8041 tgctgttgtt cagctccatc acctacggca tggcggctat cgccatgtac aaaaacaaca
     8101 aaagccaggt tatctcctgg ctggcgttga cctggttgtt tggtgctgga tttatcggga
     8161 tggaaatcta tgaattccat cacctgattg ttaacggcat gggtccggat cgcagcggct
     8221 tcctgtcagc gttctttgct ctggtcggca cgcatggtct gcacgtcact tccggtctta
     8281 tctggatggc ggtgttgatg gtgcaaatcg cccgtcgcgg cctgaccagc actaaccgta
     8341 cccgcatcat gtgcctgagc ctgttctggc acttcctgga tgtggtttgg atctgtgtgt
     8401 tcactgttgt ttatctgatg ggggcgatgt aatgagtcat tctaccgatc acagcggcgc
     8461 gtcccatggc agcgtaaaaa cctacatgac aggctttatc ctgtcgatca ttctgacggt
     8521 gattccgttc tggatggtga tgacaggggc tgcctctccg gccgtaattc tgggaacaat
     8581 cctggcaatg gcagtggtac aggttctggt gcatctggtg tgcttcctgc acatgaatac
     8641 caaatcagat gaaggctgga acatgacggc gtttgtcttc accgtgctaa tcatcgctat
     8701 cctggttgta ggctccatct ggattatgtg gaaccttaac tacaacatga tgatgcacta
     8761 agagcggcgg ttatgatgtt taagcaatac ctgcaagtaa cgaaaccagg catcatcttt
     8821 ggcaacctga tctcggtgat tgggggattc ctgctggcct caaagggccg cattgattat
     8881 cccctgttta tctacacgct ggttggggtg tcactggttg tggcgtcggg ttgtgtgttt
     8941 aacaactaca tcgacaggga tatcgacaga aagatggaaa ggacgaagaa tcgggtgctg
     9001 gttaaaggcc tgatctctcc tgctgtctcg ctggtgtacg ccaccttgct gggtattgct
     9061 ggctttatgc tgctgtggtt tggcgcgaat ccgctggcct gctggctggg ggtgatgggc
     9121 tttgtggttt acgtcggcgt ttatagcctg tacatgaaac gccactcagt ctacggcaca
     9181 ttgattggtt cgctctccgg cgctgcgccg ccggtgatcg gctactgtgc agtaaccggc
     9241 gagttcgata gtggcgcagc gatcctgctt gctatcttca gtctgtggca gatgcctcac
     9301 tcctatgcca tcgccatttt ccgctttaag gattaccagg cggcaaacat tccggtattg
     9361 ccagtggtaa aaggcatttc ggtggcgaag aatcacatca cgctgtatat catcgccttt
     9421 gccgttgcca cgctgatgct ctctcttggc ggttacgctg ggtataaata tctggtagtc
     9481 gccgcggcgg tgagcgtctg gtggttaggc atggctctgc gcggttataa agttgctgat
     9541 gacagaatct gggcgcgcaa gctgttcggc ttctctatca tcgccatcac tgccctctcg
     9601 gtgatgatgt ccgttgattt tatggtgccg gactcgcata cgctgctggc tgctgtgtgg
     9661 taacacaacc tctcagttaa aaaggtgcta cggcaccttt tttcttagca ttagaatcat
     9721 atccctctcg aaatatttac taaaaaatcc gcatgtttac cccattcgtt tgccgcttta
     9781 cactagtcgc gaatttaaaa cagaggtggt aatgaacgat tataaaatga cgccaggtga
     9841 gaggcgcgcg acctggggtt tagggaccgt attctcgttg cgcatgctgg gcatgttcat
     9901 ggttctgccg gttctgacca cgtacggcat ggctctgcaa ggtgccagcg aagcattaat
     9961 cggtattgcc attggtattt atggtctgac tcaggccgtt tttcagattc cgtttggcct
    10021 gctttccgac cgcattggtc gcaaaccatt aattgtcggt gggctggcag tgtttgccgc
    10081 cggtagcgtt atcgctgcgc tctctgactc catctgggga attattctgg gccgggcgct
    10141 acaaggctcc ggtgcgattg ccgctgccgt tatggcgctg ctttccgatc tcacgcgcga
    10201 acaaaaccgc accaaagcaa tggcgtttat cggcgtgagc tttggcatta cctttgccat
    10261 tgcgatggtg cttggcccga tcatcactca caaacttggg ctgcacgcgc tgttttggat
    10321 gatcgctatt ctggcaacaa ccggcattgc gttgaccatt tgggttgtgc ccaacagtag
    10381 cactcacgta cttaatcgtg agtccggaat ggtgaaaggc agtttcagta aagtgctggc
    10441 ggaaccgcgg ctgctgaaac tcaactttgg cattatgtgt ctgcatattt tgctgatgtc
    10501 gacgtttgtt gccctgcccg gacaactggc tgatgcgggg ttcccggcgg ctgaacactg
    10561 gaaggtctac ttggcaacga tgttaatcgc ctttggctcg gtcgtgcctt tcattatcta
    10621 cgctgaagtt aagcgcaaaa tgaagcaagt ctttgtcttc tgcgttgggt tgatcgtggt
    10681 tgcggaaatt gtgttgtgga acgcacaaac gcagttctgg caactggtgg tcggcgtgca
    10741 gcttttcttt gtggcgttta atttgatgga agccctcctg ccctcactta tcagtaaaga
    10801 gtcgccagca ggttacaaag gtacggcgat gggtgtttac tccaccagcc agtttcttgg
    10861 cgtggcgatt ggcggttcgc tgggcggctg gattgacggc atgtttgacg gtcagggcgt
    10921 atttctcgct ggcgcaatgc tggccgcagt gtggctggca gtcgccagta ccatgaaaga
    10981 accgccgtat gtcagcagct tacgcattga aatcccggcg gacattgccg caaacgaggc
    11041 gttaaaagtg cgtttgctgg aaaccgcggg ggtcaaagaa gtgttgattg cagaagaaga
    11101 acattcagct tatgtgaaaa tcgacagcaa agtgacgaat cgctttgagg ttgaacaggc
    11161 aattcgtcag gcataaaaaa cgaggcgaat tgcctgctgc gctacgctta ccaggcctac
    11221 aaagttcctg agttacaaat cgttgtaggc cgaattagct gttcatgctg cattcggcat
    11281 gaacaaaacg cacattgtcg cgattaatcg cggaagtttt tgaactggaa cggctgaccg
    11341 agatcgccac cacgtaccat cgccatgaca gcctgcaaat catcacgaga tttacccgtt
    11401 acgcggatct catcaccctg aatttgcgcc tgcactttca gtttgctgtc tttgatcatc
    11461 ttgacgattt tcttctgggt cgcgctctca atgccctgtt tcagtttcgc ttccacaaac
    11521 caggttttac cgctatgaac gatattttcc ggtacatcca gcgaactgcc ttcaatgccg
    11581 cgcttcagca gcttggcacg cagaatatcc agcaactgat tgacctggaa atcggactcg
    11641 ctcaacactt tgatggtttt gctggcgtcg ttcagctcaa atgaggcttc aacgttacgg
    11701 aagtcaaaac gggactccac ttcgcggctc gcgttatcga ccgcgttacg tgcttcctga
    11761 agatcaactt cagagacaat atcgaaagat ggcatctttt cctctccctt catttttgat
    11821 gcgaagcata atacctgcaa agttaaataa ccaacagtcc tgcaccagga cgtatccctt
    11881 aattgtgcat agtcgcaaca gcatgcacaa ttaagggata gccttattga acacccggag
    11941 tggttgcggg tgaggaggaa gaatgaaaat taccgtattg ggatgcggtg ccttagggca
    12001 attatggctt acagcacttt gtaaacaggg tcacgaagtt cagggctggc tgcgcgtacc
    12061 gcaaccttat tgtagtgtga atctggttga gacagatggt tcgatattta acgaatcgct
    12121 gaccgccaac gatcccgatt ttctcgccac cagcgatctg ctcctggtga cgctgaaagc
    12181 atggcaggtt tccgatgccg tcaaaagcct cgcgtccaca ctgcctgtaa ccacgccaat
    12241 actgttaatt cacaacggca tgggcaccat cgaagagttg caaaacattc agcagccatt
    12301 actgatgggc accaccaccc atgccgcccg ccgcgacggc aatgtcatta ttcatgtggc
    12361 aaacggtatc acgcatattg gcccggcacg gcaacaggac ggcgattaca gttatctggc
    12421 ggatattttg caaaccgtgt tacctgacgt cgcgtggcat aacaatattc gcgccgagct
    12481 gtggcgcaag ctggcagtca actgtgtgat taatccactg accgccatct ggaattgccc
    12541 gaatggtgaa ttacgtcatc atccgcaaga aattatgcag atatgcgaag aagtcgcggc
    12601 agtgatcgaa cgcgaaggac atcatacttc agcagaagat ttgcgtgatt acgtgatgca
    12661 ggtgattgat gccacagggg aaaatatctc gtcgatgttg caggatatcc gtgcgctgcg
    12721 ccacaccgaa atcgactata tcaatggttt tctcttacgc cgcgcccgcg cgcatgggat
    12781 tgccgtaccg gaaaacaccc gcctgtttga aatggtaaaa agaaaggaga gtgaatatga
    12841 gcgcatcggc actggtttgc ctcgcccctg gtagtgaaga gactgaagcc gtcaccacta
    12901 tcgacctctt ggttcgtggc ggaatcaaag tcaccactgc cagcgtcgcc agcgatggca
    12961 acctggccat tacctgctcg cgcggcgtga agctgctggc ggatgcgccg ctggtcgaag
    13021 tggctgacgg tgaatatgac gtgatcgtgc tgcctggtgg cattaaaggc gcagagtgtt
    13081 ttcgcgatag cactctgctg gttgaaaccg ttaaacagtt ccaccgttcc gggcgtatcg
    13141 tcgcggctat ttgcgccgcg ccagccaccg tgctggtgcc gcacgatatc ttcccgattg
    13201 gcaatatgac cggcttcccg acgctgaaag acaaaattcc cgccgaacaa tggcaggaca
    13261 agcgcgtcgt ctgggatgca cggataaaat tgctgaccag ccaggggccg ggtacagcta
    13321 tcgactttgg tctgaaaatt atcgacctgc tggttgggcg cgagaaagcc catgaagtgg
    13381 catcacaact ggtgatggcg gcagggattt ataattatta cgagtagtgt cggatgcggc
    13441 aaacgtcgca tctgaccaga tgcgacgtaa aaaccacaaa ttacgggcga tataccttca
    13501 cattgttaaa gccctgctcg cgcagataga gcgcctgcag gcggctcatc accccgcgct
    13561 cacaccacaa cagccaggtt ttgttctggt cgagatcgcc aaatttggtg ctcaacttgt
    13621 agaacggcag agaaaccaca tcaatccctt cgactttcag cggcttatct tcctgttcat
    13681 cgacagaacg gatatcgaga atcacgtcgt tcgggccgaa gccgttgacg gtttccactt
    13741 ccaccacttc ctgctcggtc tgctgggcga tttcgcggat atcaacgtta ttcgcttcct
    13801 caaccacttt atcgagaatg ctgaagtcga acttctcttc ttccgcttca atcttcgatt
    13861 taaccgcttt caccgtcggg cttttggaga tcacaccgca atattcgggc atcgtgcggg
    13921 caaagtcttc agtgccaatc tggcgagcca ggttgatgat gtgctctttg tcgtaagaaa
    13981 tcagcggacg caggatcagc gtgtcggaga cgttatcaat caggcgcagg ttagtcagcg
    14041 tctggctgga cacctggccc agtgcttcgc cagtgaccag cgcctgtaca ccgtaacgtt
    14101 cagccacttt agacgcggca cgcaccatca tacgtttgag gattacgccc atctgaccgt
    14161 cgtcgatttt ctcgagaatt tccccgacga ccggttcgaa attaatcgcg acaaaacgca
    14221 cgcggtggga gctgccaaag cggttccaca gataatgcgc tacctgacgc acgccaattt
    14281 catgcgccgc gccgccgagg ttaaagaagc agtaatgcac gcggcagccg cgacgcatca
    14341 acatataact ggaaacaccg gagtcgaaac caccggaaat gagcgacagc acatcttcct
    14401 gggtgccgat cgggaaaccg ccaatacctt cgtagcggcc tttaatcagc aggagacgat
    14461 cgtcttccac ttccagatgg acagtcacat ccggattggt cagcttcacg cgcgcggatt
    14521 caatatgctg atttaaaccg ccgccgacgt aacgttccac atcaatcgag ctaaaatcat
    14581 gtttgccacg gcgcttcacg cgtacgcaga aggttttccc ctccagctga tcgcgatact
    14641 gaaccaacgc tttttcgaaa atatcgtgca tgtcggtaaa cggcacgtct tcgacttcga
    14701 gaatatggtg gatacccgga atacgggtca gggcgtcgcg aatagccaga cgctggtttt
    14761 catcttttgc gcgaacttcg atgttatccc agtggcggac gacagcgagc gtctcatcat
    14821 agtgctttaa aacgttacga atgttcccgg taaggatttt tataaagcgc aagcgcacag
    14881 attggctttt gatggtgatt tccgggaaca atttaatgat aaacttcatg gcggcaatgg
    14941 ttcgttggca agccttaagc gacttgtata gggaaaaata cagcagccca cacctgcggc
    15001 tgcatccagg cgcggaagta taccactaac atcgctttgc tgcgcacatc accttaccat
    15061 tgcgcgttat ttgctatttg ccctgagtcc gttaccatga cggggcgaaa aatattgaga
    15121 gtcagacatt cattatgccg aagaaaaatg aggcgcccgc cagctttgaa aaggcgctga
    15181 gcgagctgga acagattgta acccgtctgg aaagtggcga cctgccgctg gaagaggcgc
    15241 tgaacgagtt cgaacgcggc gtgcagctgg cacgtcaggg gcaggccaaa ttacaacaag
    15301 ccgaacagcg cgtacaaatt ctgctgtctg acaatgaaga cgcctctcta acccctttta
    15361 caccggacaa tgaataatgg actttccgca gcaactcgaa gcctgcgtta agcaggccaa
    15421 ccaggcgctg agccgtttta tcgccccact gccctttcag aacactcccg tggtcgaaac
    15481 catgcagtat ggcgcattat taggtggtaa gcgcctgcga cctttcctgg tttatgccac
    15541 cggtcatatg ttcggcgtta gcacaaacac gctggacgca cccgctgccg ccgttgagtg
    15601 tatccacgct tactcattaa ttcatgatga tttaccggca atggatgatg acgatctgcg
    15661 tcgcggtttg ccgacctgcc atgtgaagtt tggcgaagcg aacgcgattc tcgctggcga
    15721 tgctttacaa acgctggcgt tctcgatttt aagcgatgca gatatgccgg aagtgtcgga
    15781 tcgcgacaga atttcgatga tttctgaact ggcgagcgcc agcggtattg ccggaatgtg
    15841 cggtggccag gcattagatt tagacgcgga aggcaaacac gtacctctgg acgcgcttga
    15901 gcgtattcat cgtcataaaa ccggcgcatt gattcgcgcc gccgttcgcc ttggtgcatt
    15961 aagcgccgga gataaagggc gtcgtgctct gccagtactc gacaagtacg cagagagcat
    16021 cggccttgcc ttccaggttc aggatgacat cctggatgtg gtaggagata ctgcaacgtt
    16081 gggaaaacgc cagggtgccg atcagcaact tggtaaaagt acctaccctg cacttctggg
    16141 tcttgagcaa gcccggaaga aagcccggga tctgatcgac gatgcccgtc agtcgctgaa
    16201 acaactggct gaacagtcac tcgatacctc ggcactggaa gcgctagcgg actacatcat
    16261 ccagcgtaat aaataaacaa taagtattaa taggcccctg atgagttttg atattgccaa
    16321 atacccgacc ctggcactgg tcgactccac ccaggagtta cgactgttgc cgaaagagag
    16381 tttaccgaaa ctctgcgacg aactgcgccg ctatttactc gacagcgtga gccgttccag
    16441 cgggcacttc gcctccgggc tgggcacggt cgaactgacc gtggcgctgc actatgtcta
    16501 caacaccccg tttgaccaat tgatttggga tgtggggcat caggcttatc cgcataaaat
    16561 tttgaccgga cgccgcgaca aaatcggcac catccgtcag aaaggcggcc tgcacccgtt
    16621 cccgtggcgc ggcgaaagcg aatatgacgt attaagcgtc gggcattcat caacctccat
    16681 cagtgccgga attggtattg cggtcgctgc cgaaaaagaa ggcaaaaatc gccgcaccgt
    16741 ttgtgtgatt ggcgacggtg cgattaccgc tggcatggcg tttgaagcga tgaatcatgc
    16801 gggcgatatc cgtcctgata tgctggtggt tctcaacgac aatgaaatgt cgatttccga
    16861 aaatgttggc gcgctcaaca accatctggc gcagctgctt tccggtaagc tttactcttc
    16921 actgcgcgaa ggcgggaaaa aagttttctc tggcgtgccc cccattaaag agctgctcaa
    16981 acgtaccgaa gaacatatta aaggcatggt agtgcctggc acattgtttg aagagctggg
    17041 ctttaactac atcggcccgg ttgacggtca cgatgtgctg gggcttatca ccacgcttaa
    17101 gaacatgcgc gacctgaaag gcccgcagtt cctgcatatc atgaccaaaa aaggtcgtgg
    17161 ttatgaaccg gcagaaaaag acccaatcac tttccacgcc gtgcctaaat ttgatccctc
    17221 cagcggttgt ctgccgaaaa gtagcggcgg tttaccaagc tattcaaaaa tctttggcga
    17281 ctggttgtgc gaaacggcag cgaaagataa caagctgatg gcgattactc cggcgatgcg
    17341 tgaaggttcc ggcatggtcg agttttcacg taaattcccg gatcgctact tcgatgtggc
    17401 aattgccgag caacacgcag tgacctttgc tgcgggtctg gcgattggtg ggtacaaacc
    17461 cattgtcgcg atctactcca ccttcctgca acgcgcctat gatcaggtgc tgcatgacgt
    17521 ggcgattcaa aagcttccgg tcctgttcgc catcgaccgc gcgggcattg ttggtgctga
    17581 tggtcaaacc caccagggcg cttttgatct ctcttacctg cgctgtattc cggaaatggt
    17641 cattatgacc ccgagcgatg aaaacgaatg tcgccagatg ctctataccg gctatcacta
    17701 taacgatggc ccgtcagcgg tacgctaccc gcgtggcaac gcggttggcg tggaactgac
    17761 gccgctggaa aaactgccaa ttggcaaagg cattgtgaag cgtcgtggcg agaaactggc
    17821 gatccttaac tttggtacgc tgatgccaga agcggcgaaa gtcgctgaat cgctgaacgc
    17881 tacgctggtc gatatgcgtt ttgtgaaacc gcttgatgaa gcgttaattc tggaaatggc
    17941 cgccagccat gaagcgctgg tcaccgtaga agaaaacgcc attatgggcg gcgcaggcag
    18001 cggagtgaac gaagtgctga tggcccatcg taaaccagta cccgtgctga acattggcct
    18061 gcctgacttc tttattccac aaggaacgca ggaagaaatg cgcgccgaac tcggcctcga
    18121 tgccgccggt atggaagcca aaatcaaggc ctggctggca taatccctac tccactcctg
    18181 ctatgcttaa gaaattattc atggcaggag tggcagcatg caatacaacc ccttaggaaa
    18241 aaccgacctt cgcgtttccc gactttgcct cggctgtatg acctttggcg agccagatcg
    18301 tggtaatcac gcatggacac tgccggaaga aagcagccgt cccataatta aacgcgcgct
    18361 ggaaggcggc ataaatttct ttgacaccgc caatagctat tccgatggca gcagcgaaga
    18421 gatcgtcggt cgcgcactac gggatttcgc ccgtcgtgaa gacgtggtcg ttgccaccaa
    18481 agtgttccat cgcgttggtg atttaccgga aggattatcc cgtgcgcaaa ttctacgttc
    18541 tatcgacgac agcctgcgcc gtctcggcat ggattatgtc gatatcctgc aaattcatcg
    18601 ctgggattac aacacgccga tcgaagagac gctggaagcc ctgaacgacg tggtaaaagc
    18661 cgggaaagcg cgttatatcg gcgcgtcatc catgcacgct tcgcagtttg ctcaggcact
    18721 ggaactacaa aaacagcacg gctgggcgca gtttgtcagt atgcaggatc actacaatct
    18781 gatttatcga gaagaagagc gcgagatgct gccactgtgt taccaggagg gcgtagcggt
    18841 gattccatgg agcccgctgg cgcggggtcg tctgacacgt ccgtggggag aaactaccgc
    18901 acgactggtg tctgatgagg tggggaaaaa tctctataaa gaaagcgatg aaaatgacgc
    18961 gcagatcgca gagcggttaa cgggcgtcag tgaagaactg ggtgcaacac gagcacaagt
    19021 tgcgctggcc tggttgttga gtaaacgggg cattgccgca ccgattatcg gaacttcgcg
    19081 ggaagaacag cttgatgagc tgctgaacgc ggtggatatc accttgaagc cggaacagat
    19141 tgctgaactg gaaacgccgt ataaaccgca tccggtcgta ggatttaagt aaatttcgct
    19201 tttcaaccat tttatctgct gaattcccca tcgtctgcga tgatggggac aacgtacctc
    19261 ccatcatctt ctgtaaccat aatccacctc tgcaagagaa attcttggtg aatgagtttg
    19321 cagctattca tcatcttcat ccctttgagc gatttttgat agtttctcca ggtaatactc
    19381 cctaaaactg tcttatttag tcaacgtaag aggattacaa tcatgatgaa aactatcacc
    19441 aaacaaccga tcctgtttac tgatgtaccg gttgcagatt taagaaactc aatgaagcac
    19501 gatttaaatc aaaatcttat cgaaaggcta tggaacaaaa ttcgtgattt ttttctggac
    19561 agtgataaac aaaaggcatt taaatcgatt cataaataca tcaatacatt gtcagttcta
    19621 aattataatt cagcgttaac tccagatccc aattttaata tagatgcatc gtcagattta
    19681 gactcatatt taaacctgaa atttgataac ctctcgccca aacaaaagca gaccacgctt
    19741 tgttgtttct ggaataaaat tgcatcctca ttgccagagc catacaacag caccatcaaa
    19801 cataacatta ttttttataa agtcggagaa aatctgatga tacgtggaac tatctctatt
    19861 gttaacgagg tagtaaagac ttattcactc ccaatagaaa aggatgacaa tggatattac
    19921 gatttcagcg gtctatatct agcacacagc aatatttccg gtaaagatcc caataaagat
    19981 cctgctatag atttcggtat agatctggga aactgtaact gctcaaatgt caacttcgaa
    20041 catacttact ttgatagcgt caagtttaca aatacgaatt gtacaaatgc gaattttaac
    20101 agttgtagat ttatcaaatg cgatctaaca aatatgaatt gcactggcgc aattcttgat
    20161 aatgcggtga tatatggtaa agaaaaagaa cctgagatgc aatatccaga agcagatcaa
    20221 ataatacaaa ggataactta tcagaaatca catgggaatg aaacgaaagg gatgattcta
    20281 actaactgct cctgtgttaa aaccactttc aattgggcag atttatcaga aagtgattgc
    20341 caaaatgtgg actttagcga ggctaatctt tcaaacacta ttcttcctga catcgtcagg
    20401 atgaaaggca cgaaacttta ccgtaccgac ctttttaatc cgatattaaa agcagaagca
    20461 gaatcaacag aagaaaaaga tattagccca ttagcaaaaa ttattcttga ctatattgaa
    20521 tcagataaaa accctgagtc attaaaattt gatgaaaaat caacaaccac taaaataata
    20581 caagatatcg ataattttat tttctataat aagcatttaa agagcatatt caatcaagca
    20641 atgaatcttg aaggaaaaat aagcagaaaa aaatacaatg agatctttaa ctataaacaa
    20701 gaacaagcca gacaatattt catcgaccag tataaactaa caaaaaatga ttatttgaag
    20761 aaaatacctt taactgcaca attaattgca aaatacaaaa tggatgatca attggaccaa
    20821 cttttggtta cccgtgaaat tcaagatgaa ataaaaccaa agattcaaga caaaatagat
    20881 gaactttcta aaaatttatt caacactata aaccaaacca ttaaggataa ctttgatgat
    20941 atttttcggc aacagtcaga gaatatgagc aattattatg agttcgtcga ctgacgatat
    21001 ttgagtgtac gtgaattatg agtgacaaag atacaccttg aaatatctcc gcataatata
    21061 ttcggtgatg tcggatttta ctttaacaga gtgcctcaaa attggggcac tgttcattta
    21121 taaatcaaac cgggtggtct ggaatcgact acaccacccg gtcaatggca tcaaagaata
    21181 cccagcggcc aatgatgacc gataaaatac aggatgccag cggagatcac cccggcgaca
    21241 atatcatcta tcatgatccc catgccgcca tgtacattgc ggtcaaacca gcggatcggc
    21301 cacggtttcc acatatccag gatacggaaa atcacaaacc cggcggcgac ccactgccag
    21361 tcattggtcg gcagcgccat gagcgtgatc cacataccaa taaattcgtc ccagacaatg
    21421 ctgccgtgat cgtgcacgcc catgtctttc gccgtttgat gacaaagata gacgccgata
    21481 cagatcccca gcatcaccac cagcgaatag agctgccagg gcaaaaaggt catcagatac
    21541 cagaacggaa tcgccgccag cgatcccatc gtgccaggaa cgattgggct taatccactt
    21601 ccgaatccga cagcaagcag atgccacgga ttactcatct tcaggcgact tttcgcgaca
    21661 tctttatggc gtggcaaaat ggtcatatcc tttccagtct aacgtgacag gttcgccgtc
    21721 acgaataaaa caaagccctt cgatatcggc ggtcatttgc ccgatacagg taaacggtac
    21781 gcccagatgt ccgagagcca catccagcgc gccacggttc agttccggca ccgtgaaaca
    21841 caactcgtaa tcttcaccgc cagagagcgc ccagcgcagc gcctgttccg gttcaacatg
    21901 gcgagaaagc gcatcagaaa acggcagcaa tgccaggtca atacgtgcgc cgcagtcgct
    21961 ggctttcacg atatgcccga gatcggaaat cagaccgtca gagagatcga tagctgaatt
    22021 tgccagatcg cgcagtgcct gtccctgtaa aatacgcgga gatggacgga gatgacgttt
    22081 gatcaagtag tccgcatctt taacatcggc aacctgcaaa cggttttgca aaatcgccag
    22141 cccggcggcg ctatcgcccg gtgtaccggt cacatagatc cagtcacccg gtttcgcccc
    22201 agcgcgcgtt aaggctcgtc ccatcggaac aaagccgtgg atacccagcg tcattgataa
    22261 tggcccacgc gtggtatcgc cgccaatgag ttgcatatcg taataattga gaagatcaaa
    22321 caaactgtcg ctgaaggact caagccacgc ttcgtcgacg tccggtaagg ttaatgccag
    22381 cgtcagccag gctggatcgg cccccatcgc tgccagatcg cttaggttca ccgccagtgc
    22441 tttatacgcc agatcagcag gatcgatatc agggaggaag tggttgcccg ccaccagcgt
    22501 atcagtgctg atcgccaggg tctgtttctc ggggatattg agaagtgcgc aatcgtcgcc
    22561 aatgcccagt tcgacatcaa gacgagaact tcttacacgg tcaaaataac gggcaatcag
    22621 ggagaactcg ccacatgcca tacgttatgc ctcagcaggt aaaaaagaaa aggccggcga
    22681 tcgcggaacg gtcttccgtg aatctaccgg cctggatatc actttttgtt agggcgaatc
    22741 acaggtgctg ctttatcgag tacgccgttg acgaacttat ggctgtcttc tgcgccgaac
    22801 gatttcgcca gttcgatcgc ttcgttaatg gccactttgt atggcacatc gctacgttta
    22861 gacagctcat acagcgcaat gcgcagtact gctttttcta cctggcccag ttcttccagc
    22921 aggcgggaca ggtatggctt catcagtccg tcgaggtatg cggtgttagt cgccaccccg
    22981 gccagcagct cacggaagta caggacgtca acgtctttta catcctgttc agccaggaac
    23041 tggtattcaa catcagcgat gtcgttctgg gacaactgcc aggagtagag cgcctggacg
    23101 gcacactcac gagcgcggcg acgagcagca ggtttcacgg atttcccctt actaatttca
    23161 ggccttgatg gctttcaata cattaatcat ttcaagcgcg gtcagtgcag cttctgcacc
    23221 tttgttgcca gctttggtgc cagcacgttc gatcgcttgt tcaatgcttt cagtggtcag
    23281 aaccccaaaa gcaaccggaa tttcgctgtc ctgggcaaca tgcgccaggc cgttgcttgc
    23341 accaccagcg acatattcaa agtgggcagt gccaccacga ataaccgtac ccagcgcaat
    23401 caccgcgtcg tatttaccgg ttttagccag tgcacccgcc gccagcggca gctcataggc
    23461 accaggcacc caaacaacgg taatgttttc atcttttacc tgaccgatac gtttcagtgc
    23521 gtcaattgca ccttccagca ggctgtcatt gataaagttg ttgaaacgcg cgatggtgat
    23581 ggcgacgcga gcgtccgggg tagcaacgtt agcttcaata atgttcattt tcttccttcg
    23641 ggttcgagta tggccccgca ggggggcgga ttttagcata atatttcgtg cgctgcttcc
    23701 ctttcgagcc gggagatcat gcacccacta aatgcaggca aacatccggg cctacatgac
    23761 gtatctcttt gaatttaaat tggggggcgt cagctaattt ctcaagccct ggcagcgtgc
    23821 ataatccacg ggcgtcgctg cctaatagtt taggtgcgat atagacaatc agctcatcga
    23881 ctaaacccgc ctgcagcaat gcgccagcga gcgttggccc cgcttccacc cagatgctgt
    23941 taatctgctg tttacccagt tgcatcatta gtacaaccag atccagatga cctttatgct
    24001 ctggaatcag caaggtacgt accgtttccg gccactcacg agaatcttcc tgcgtacgcg
    24061 cgaaccaggt ttcgccgggc tgctgcacaa tgcgatgttc cggcgtcacg cgattttggc
    24121 tatcaatcac aatacgtatc ggctgacgga gattttgttg cggatagagc gcctgagttt
    24181 gttcatccag ttcagaccaa cgcactgtta aggctggatc atccgccagc accgtggcgc
    24241 tgctggttaa aatggcatga ctttgtgcgc gcagtcgttg tacgtcgcgc cgcgcctgag
    24301 gcgaagtgat ccactggctt tcgccgctcg ccatcgccgt gcgaccatca agcgatgcgc
    24361 caagttttaa ttgaatataa ggaaagccgg tgcgcatccg cttgagaaag cctttattca
    24421 attgctcggc ttcactcatc atcagcccgt ggctgacgtc aatgccagcc tgttgcagac
    24481 gatatagccc tcgcccagct acctgcgggt taggatcttg catcgaggca accacgcgcg
    24541 ccacacctgc ggcaattaat gcatcacagc aaggcggcgt acggccgtga tggctgcaag
    24601 gttcgagtgt cacatacgca gtcgcacctt tggctttatc tccagccata cgcaacgcat
    24661 gtacttcggc gtgtggttca ccagcacggt gatggtaacc ttcaccgaca atttcgccat
    24721 ctttgacaat gacgcacccg acattcgggt taggatgcgt ggtaaaacgt cctcgttgcg
    24781 ccagctttag cgcccgcgcc atgtaatact cgtcctgcac ggcttagtcc tccaggcgcg
    24841 cgatctcttc gccaaattct ttgatatctt cgaaactgcg gtagacagag gcaaaacgga
    24901 tataggcgac tttatcgagc tttttcaatt gctccatcac cagattgcca atcatcttgc
    24961 tcggcacttc gcgctcaccg gtggcgcgca gctgcgattt aatatgattg attgccattt
    25021 cgacgtcatc ggaactcacc ggacgttttt ccagcgcccg cagcattccg ctacgcaatt
    25081 tctcttcatt aaacggttca cgcacgtcgt tgctttttac aacacgcggc ataaccagct
    25141 ccgccacttc aaaggtggtg aaacgttcat tacacaccag acactgccga cggcggcgta
    25201 cagatgagcc ctcgccaacg agacgagagt caattacctt agtgtccacg gcgaaacaga
    25261 atgggcaatg catacggcgt cctgaccagg tggttaacag aaatctattt taccctgaac
    25321 tgacgataca acaaaggcgc aacgcttttg agataaagga tcgatgcttt cgctgctttt
    25381 accctctacc attaggctaa ttgcgacgag aaagggacga cctatgaata ctaacgtttt
    25441 tcgactgctc ctgctgggaa gcctgttcag ccttagcgcc tgtgtgcagc aaagcgaagt
    25501 gcgacagatg aaacacagcg tcagcacgct gaaccaggag atgacgcaac tcattgacat
    25561 cctccacgcc ctgaaggacg tggatccctg ctacgttcag gctgtcgcct gaatcatttc
    25621 ggtgggttcc tgcttcaacg agcggcctca ctgcaccatc tctccacagg caagaacggc
    25681 gtgccccacc gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca
    25741 tacctggcat ctgaattgac tttgtgacag gcgattttct ttcgctgtat gaccacagca
    25801 cgcgcaacgc tggcttgtgt acgctggcgg agcagcaagc acctgaccgc cacgccagag
    25861 ctgcttgtac tcaagctggc ggcgcatttc ataccagccc tgatccagta tcg
//
LOCUS       DAPOZO010000068        25799 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPOZO010000068 DAPOZO010000000
VERSION     DAPOZO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.068"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..2657
                     /locus_tag="ROI21_003089"
     CDS             <1..2657
                     /locus_tag="ROI21_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HDX5111983.1"
                     /translation="LAGTVSSDSTDAINGSQLYGLADSFTSYLGGGADISDAGVLTGP
                     TYTIGGTDYNNVGDALAAINTSFSTSLGDALLWDATAKGGDGAFSAGRGTDNTASIIT
                     NVADGAISSTSSDAINGSQLYDTSKYIADTLGGNAEVNADGTITAPTYAIAGGSYSNV
                     GDALEAIDTTLDDALLWDATANDGNGAFSAGRGVDKTASVITNVADGAISSTSSDAIN
                     GSQLYTTNKYIADALGGDAEVNADGSITAPSYTIANAEYNNVGDALDALDDNALLWDA
                     TANDGAGAYNASHDGKASIITNVADGNIGEGSTDAINGSQLFNTNMLIQQNSEIINQL
                     AGNTSETYIEDNGAGINYVRTNDNGLAFNDASASGIGATAVGYNAVASGESSVAIGQG
                     SSSTVDTGIALGSSSVSSRVIVKGSRDTSVSEEGVVIGYDTTDGELLGALSIGDDGKY
                     RQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGEDSLAMGAK
                     TIVNGNAGIGIGYGAYVDANALNGIAIGSNARANHANSIAMGNGSQTTRGAQTGYAAY
                     NMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNL
                     NNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAAD
                     NSVALGTGSVANEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDT
                     KADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVE
                     MDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSA
                     NGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            2955..4610
                     /gene="lldP"
                     /locus_tag="ROI21_003090"
     CDS             2955..4610
                     /gene="lldP"
                     /locus_tag="ROI21_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDX5111984.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVIYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4610..5386
                     /gene="lldR"
                     /locus_tag="ROI21_003091"
     CDS             4610..5386
                     /gene="lldR"
                     /locus_tag="ROI21_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDX5111985.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5383..6573
                     /gene="lldD"
                     /locus_tag="ROI21_003092"
     CDS             5383..6573
                     /gene="lldD"
                     /locus_tag="ROI21_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDX5111986.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSGLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGRELPAALAPMAK
                     GNAA"
     gene            6646..7119
                     /gene="trmL"
                     /locus_tag="ROI21_003093"
     CDS             6646..7119
                     /gene="trmL"
                     /locus_tag="ROI21_003093"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDX5111987.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7172..7993)
                     /gene="cysE"
                     /locus_tag="ROI21_003094"
     CDS             complement(7172..7993)
                     /gene="cysE"
                     /locus_tag="ROI21_003094"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDX5111988.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8073..9092)
                     /gene="gpsA"
                     /locus_tag="ROI21_003095"
     CDS             complement(8073..9092)
                     /gene="gpsA"
                     /locus_tag="ROI21_003095"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDX5111989.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9092..9559)
                     /gene="secB"
                     /locus_tag="ROI21_003096"
     CDS             complement(9092..9559)
                     /gene="secB"
                     /locus_tag="ROI21_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDX5111990.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(9622..9873)
                     /gene="grxC"
                     /locus_tag="ROI21_003097"
     CDS             complement(9622..9873)
                     /gene="grxC"
                     /locus_tag="ROI21_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDX5111991.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10015..10446)
                     /locus_tag="ROI21_003098"
     CDS             complement(10015..10446)
                     /locus_tag="ROI21_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDX5111992.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            10691..12235
                     /gene="gpmM"
                     /locus_tag="ROI21_003099"
                     /gene_synonym="pgmI"
     CDS             10691..12235
                     /gene="gpmM"
                     /locus_tag="ROI21_003099"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDX5111993.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12245..13528
                     /gene="envC"
                     /locus_tag="ROI21_003100"
     CDS             12245..13528
                     /gene="envC"
                     /locus_tag="ROI21_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDX5111994.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRSSLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13532..14491
                     /locus_tag="ROI21_003101"
     CDS             13532..14491
                     /locus_tag="ROI21_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDX5111995.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(14478..15512)
                     /gene="waaH"
                     /locus_tag="ROI21_003102"
     CDS             complement(14478..15512)
                     /gene="waaH"
                     /locus_tag="ROI21_003102"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDX5111996.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(15751..16776)
                     /gene="tdh"
                     /locus_tag="ROI21_003103"
     CDS             complement(15751..16776)
                     /gene="tdh"
                     /locus_tag="ROI21_003103"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDX5111997.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(16786..17982)
                     /gene="kbl"
                     /locus_tag="ROI21_003104"
     CDS             complement(16786..17982)
                     /gene="kbl"
                     /locus_tag="ROI21_003104"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDX5111998.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18196..19128
                     /gene="rfaD"
                     /locus_tag="ROI21_003105"
     CDS             18196..19128
                     /gene="rfaD"
                     /locus_tag="ROI21_003105"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDX5111999.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19138..20184
                     /gene="rfaF"
                     /locus_tag="ROI21_003106"
     CDS             19138..20184
                     /gene="rfaF"
                     /locus_tag="ROI21_003106"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDX5112000.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20188..21147
                     /gene="rfaC"
                     /locus_tag="ROI21_003107"
     CDS             20188..21147
                     /gene="rfaC"
                     /locus_tag="ROI21_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDX5112001.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTVREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVVCRAPLMNLNNLEAAAVYKKITLI"
     gene            complement(21195..22448)
                     /locus_tag="ROI21_003108"
     CDS             complement(21195..22448)
                     /locus_tag="ROI21_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDX5112002.1"
                     /translation="MEVKIEQFRNNSCNFIFLLYLIGLLFVPIDLNITTKIINATGAF
                     SLFLLIVNKKHNYNKNVIILILGLLLLGAMNLIWYEFYKTEDVVYRNAYRGYLESGKL
                     LVFSSLTFLLLSKNKFRQNEKLHFISAILVQIIMLCRAYYQGIYLHADRIPLSAMNGN
                     IGQMGAATIAAYVITFTTLYSSIVFIKMQSKYKWFLFYANFALSFAAVMMTGTRAAIF
                     TFPLMIMVILFLQHRDQKVFLFKGLSGVFILLLACGLIFNKEIDRRINSLKADVISYA
                     TKNNSQSSVGARFAMVNAGIKGSPDGFNWQSLEQRAEKIKALSAENNIYSGALLFLDV
                     HMHNEIVESLSTKGKIGLLVLIMFYVAMIYYCIREKKYILLVFPASIMLFGISDVITH
                     AKPIPASWIVCLFLSISFLSKNEPQ"
     gene            complement(22483..23255)
                     /locus_tag="ROI21_003109"
                     /pseudo
     CDS             complement(22483..23255)
                     /locus_tag="ROI21_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
     gene            complement(23287..24315)
                     /locus_tag="ROI21_003110"
     CDS             complement(23287..24315)
                     /locus_tag="ROI21_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HDX5112003.1"
                     /translation="MDLLAESITEVTVSGKITNTDRVLNIAYGIDRNFLFGAAVSMQS
                     VIMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSEVIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKSRHLKRNGNIASSFYYFMLYYSKK
                     IARKHFSK"
     gene            complement(24341..25033)
                     /gene="rfaY"
                     /locus_tag="ROI21_003111"
     CDS             complement(24341..25033)
                     /gene="rfaY"
                     /locus_tag="ROI21_003111"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDX5112004.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFFKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKFFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            complement(25043..>25799)
                     /locus_tag="ROI21_003112"
     CDS             complement(25043..>25799)
                     /locus_tag="ROI21_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HDX5112005.1"
                     /translation="IIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLYL
                     DADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVN
                     LKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNE
                     LYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEA
                     RTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT         6664 a         6454 c         6267 g         6414 t
ORIGIN      
        1 tactggcagg cactgtatcc tctgacagca ctgacgccat taatggctca caactttatg
       61 gcttagctga ttcatttacg tcataccttg gcggtggtgc tgatatcagc gatgcgggtg
      121 tattaaccgg gccaacctac actattggtg gtactgacta caataacgtc ggtgatgctc
      181 tggctgccat taacacgtca tttagcacat cactgggtga tgccctactc tgggatgcga
      241 ccgccaaggg cggtgacggt gcgtttagcg ccggtcgcgg gacagataac acggccagca
      301 tcattactaa tgttgcagac ggtgcaattt cctctaccag cagcgacgct attaacggct
      361 cacagctcta tgacaccagc aagtacattg ccgatactct gggtggtaac gcagaagtca
      421 atgctgacgg cacaatcacc gcaccgactt atgccatcgc tggtggcagt tacagcaacg
      481 tcggtgacgc gctggaagcg atcgatacca cgctggatga cgccctgctg tgggatgcca
      541 cagccaatga tggtaatggt gcatttagcg ccggtcgcgg agtagataaa acggccagcg
      601 tcattactaa cgtcgcagac ggtgcaattt cctccaccag cagcgatgct attaacggct
      661 cacaactgta taccaccaac aagtacatcg ctgatgcgct gggtggtgac gcagaagtca
      721 acgctgatgg ttctattact gcgccgagtt acaccattgc aaatgccgag tacaacaacg
      781 tcggtgacgc cctggatgcg ctcgacgata acgctctgct gtgggatgca acggccaatg
      841 acggcgcagg tgcctacaac gccagccatg atggcaaggc cagcatcatc acaaacgttg
      901 ctgatggtaa cattggcgaa ggcagcactg acgccatcaa cggttctcag ctgtttaaca
      961 ccaatatgct gatccagcag aacagcgaaa tcatcaatca gcttgccggt aacaccagcg
     1021 aaacctacat cgaagataac ggtgcgggca ttaactatgt acgtaccaac gacaacggct
     1081 tagcgttcaa cgatgccagc gcttcaggta ttggcgctac ggctgtgggt tataacgctg
     1141 tcgcctcagg cgaaagcagc gtagccattg gtcaaggtag cagcagcacc gttgataccg
     1201 gtattgccct gggtagcagt tccgtttcca gccgtgtaat agttaaaggt tctcgtgaca
     1261 ccagcgtgtc ggaagaaggt gttgtgattg gttatgacac cacggatggc gaactacttg
     1321 gtgcgttgtc tattggtgat gacggtaaat atcgtcaaat catcaacgta gccgatggtt
     1381 ccgaagccca tgacgccgtt acggttcgcc agttgcaaaa tgccattggt gcagtcgcta
     1441 ccacgccgac caaatacttc cacgccaact caacggaaga agattcactg gcagtaggtg
     1501 aagactcgct agcaatgggc gcgaaaacta tcgttaatgg taatgcgggt attggtatcg
     1561 gttatggtgc ctacgtggac gcgaatgcac ttaatggcat tgctatcggt agcaacgcgc
     1621 gtgcaaacca tgcaaacagc attgctatgg gtaatggctc acagacgact cgtggtgctc
     1681 aaactggcta cgccgcctac aacatggacg caccgcagaa ctctgtgggt gagttctctg
     1741 tcggcagtga agacggtcaa cgtcagatca ccaacgtcgc ggctggttcg gctgataccg
     1801 atgcggttaa cgtgggtcag ttgaaagtaa cggacgcgca ggtttcccag aatacccaga
     1861 gcattactaa cctgaacaat caggtgacga atctggatac tcgcgttacc aatatcgaaa
     1921 acggcattgg cgatatcgta accaccggta gcaccaaata cttcaagacc aacaccgatg
     1981 gcgtagatgc caacgcgcag ggtaaagaca gtgttgcaat tggttctggt tccattgctg
     2041 ccgctgacaa cagcgtcgcg ctgggtaccg gttccgtggc caacgaagaa aacaccatct
     2101 ctgtgggttc ttctaccaac cagcgtcgta tcaccaacgt tgctgcaggt gttaatgcca
     2161 ccgatgcggt taacgtttcg cagctgaagt cttctgaggc aggcggcgtt cgttacgaca
     2221 ccaaagctga tggttctata gactacagca acatcactct cggtggtggt aatggcggta
     2281 cgactcgcat cagcaacgtc tccgctggcg tgaacaacaa cgacgcagtg aactatgcgc
     2341 agctgaagca aagcgtgcag gaaacgaagc aatataccga tcagcggatg gttgagatgg
     2401 ataacaaact gtccaaaacc gaaagcaagt tgagcggtgg tatcgcttct gcaatggcaa
     2461 tgaccggtct gccgcaggct tacactccag gtgccagtat ggcctctatt ggtggcggta
     2521 cttacaacgg tgaatcggct gttgctttag gtgtgtcgat ggtgagcgcc aatggtcgtt
     2581 gggtctacaa attacaaggt agtaccaata gccagggtga atactccgcc gcactcggtg
     2641 ccggtattca gtggtaatga tccattaact tagtgtgact aagggcaatt ctctgatgag
     2701 gattgccctt ttctttacca gacatctccc tccaccaaga attggcccta ccaattcttc
     2761 gcttatctga cctctggttc acaattcccc aattaaaact cacatcaatg ttgccaatgc
     2821 ataacattta gttaaccatt cattgtcatt atccctacac aacacaattg gcagtgccac
     2881 ttttacacaa cgtgtgacaa ggagatgagc atcagactca ttacacgatg tgcgtggact
     2941 ccaggagacc tgcaatgaat ctctggcaac aaaactacga tcccgccggg aatatctggc
     3001 tttccagcct gatagcatcg cttcccatcc tgtttttctt ctttgcgctg attaagctca
     3061 aactcaaagg ttatgtcgcc gcctcatgga cggtggcaat cgcccttgcc gtggctttgc
     3121 tgttctataa aatgccggtc gctaacgcgc tggcctcggt gatttatggc ttcttctacg
     3181 gtttgtggcc catcgcgtgg atcattattg cggcagtgtt cgtctataag atctcggtga
     3241 aaaccgggca gtttgacatc atccgctcgt ctattctttc gataacccct gaccagcgcc
     3301 tgcaaatgct gatcgtcggt ttctgtttcg gcgctttcct tgagggagcc gcaggctttg
     3361 gcgctccggt agcaattacc gccgcattac tggtcggtct gggctttaaa ccactgtacg
     3421 ccgccggact gtgcctgatt gttaacaccg cgccagtggc atttggcgcg atgggtattc
     3481 cgattctggt cgccgggcag gtaacaggca tcgacagctt tgagattggt cagatggtgg
     3541 ggcgtcagct gccgtttatg accattatcg tgctgttctg gatcatggcg attatggacg
     3601 gttggcgcgg tatcaaagag acgtggcctg cggtcgtggt tgcaggtggt tcgtttgcca
     3661 ttgctcaata cctcagctct aacttcattg ggccggaact gccagacatt atctcttcgc
     3721 tggtatcact gctctgcctg acgctgttcc tcaaacgctg gcaaccagtg cgcgtattcc
     3781 gcttcggtga cttgggggcg tcacaggttg atatgacgct ggctcacacc ggttacaccg
     3841 caggtcaggt gcttcgcgcc tggacaccgt tcctgttcct gaccgccacc gtaacgctgt
     3901 ggagtattcc accgtttaaa gccctgttcg cttcgggtgg tgcgctgtat gaatgggtga
     3961 tcaatattcc ggtgccgtac ctcaataaac tggttgcccg tatgccgcca gtggtcagcg
     4021 aagcgacagc gtatgccgcc gtgtttaagt tcgactggtt ctctgccacc ggtaccgcca
     4081 ttctttttgc tgccctgctc tcgattgtct ggctgaagat gaaaccatct gacgctatca
     4141 gcaccttcgg cagcacgctg aaagaactgg ctctgcctat ctactccatc ggtatggtgc
     4201 tggcgttcgc ctttatctcg aactattccg gactatcatc aacgctggcg ctggcactgg
     4261 cgcacaccgg ccatgcattc accttcttct cgccgttcct cggctggctt ggtgtcttcc
     4321 tgaccggatc ggatacctca tctaacgccc tgttcgccgc gctgcaagcc actgcagcac
     4381 aacaaattgg cgtttctgac ctgttgttgg ttgccgccaa caccaccggt ggtgtcaccg
     4441 gtaagatgat ctctccgcaa tctatcgcta tcgcctgtgc ggcggtaggc ctggtaggca
     4501 aagagtcaga tctgttccgc tttactgtca aacacagcct gatcttcacc tgtatggtgg
     4561 gcgtgatcac cacgcttcag gcttatgtct taacgtggat gattccttaa tgattgtttt
     4621 acccagacgc ctgtcagacg aggttgccga tcgtgtgcgg gcgctgattg atgaaaaaaa
     4681 cctggaggcg ggcatgaagt tgcccgctga acgtcaactg gcgatgcaac tcggcgtatc
     4741 gcgtaattca ctgcgcgagg cgctggcaaa actggtgagc gaaggcgtgc tgcttagtcg
     4801 acgcggcggc gggacgttta ttcgctggcg tcatgacaca tggtcggagc aaaacatcgt
     4861 ccagccgctg aaaacgctga tggccgatga tccggattac agtttcgata ttctggaagc
     4921 ccgctacgcc attgaagcca gcaccgcatg gcatgcagca atgcgcgcca caccaggcga
     4981 aaaagagaaa attcagcttt gctttgaagc aacgctaagt gaagacccgg atctcgcctc
     5041 acaagcggac gttcgttttc atctggcgat tgccgaagcc tcacataaca tcgtgctgct
     5101 gcaaaccatg cgcggtttct tcgatgtcct gcaatcctct gtgaagcata gccgccagcg
     5161 gatgtatctg gtacccccgg ttttttcaca gctgaccgaa caacatcagg cagtcattga
     5221 cgccattttt gccggtgatg ctgacggggc gcgtaaagca atgatggcgc accttagctt
     5281 tgttcacacc accatgaaac gattcgatga agatcaagct cgccatgcac gcattacccg
     5341 cctgcctggt gaccataatg agcattcgag ggagaaaaac gcatgattat ttccgcagcc
     5401 agcgattatc gcgccgcagc acaacgcatt ctgccgccgt tcctgttcca ctatatggat
     5461 gggggtgcat attctgaata cacgctgcgc cgcaacgtgg aagatttgtc agaagtggcg
     5521 ctgcgccagc gtattctgaa aaacatgtct ggcttaagcc tggaaacgac gctgtttaat
     5581 gagaaattgt cgatgccggt ggcgctggct ccggtgggtt tgtgtggcat gtatgcgcgt
     5641 cgtggcgaag ttcaggctgc caaagcggcg gatgcgcatg gtattccgtt tactctctcg
     5701 acggtttccg tttgcccgat tgaagaagtc gctccagcca ttaaacgtcc gatgtggttc
     5761 cagctttatg tgctgcgcga tcgcggcttt atgcgtaacg cgctggagcg agcaaaagca
     5821 gcgggttgtt cgacgctggt tttcaccgtg gatatgccga caccgggcgc gcgttaccgt
     5881 gatgcacact cgggtatgag cggcccaaat gcggcaatgc gccgttactt gcaggcggtg
     5941 acgcatccgc aatgggcgtg ggatgtgggc ctgaacggtc gtccacatga tttaggtaat
     6001 atctcggctt acctcggcaa accgaccgga ctggaagatt acatcggctg gctggggaat
     6061 aacttcgatc catccatctc atggaaagac cttgagtgga tccgcgattt ctgggatggt
     6121 ccgatggtga tcaaagggat cctcgatccg gaagatgcgc gcgatgcagt acgttttggt
     6181 gctgatggga ttgtggtttc taaccacggt ggccgccagc tggacggtgt actctcttcc
     6241 gctcgtgcat tgcccgctat tgcggatgcg gtgaaaggtg atattgccat tctggcagat
     6301 agcgggattc gtaacgggct tgatgtcgtg cgtatgattg cgctcggtgc cgacaccgta
     6361 ctgctgggtc gtgctttcct gtatgcgctg gcaacagcgg gccaggcggg cgtggccaat
     6421 ctactgaacc tgatcgagaa agagatgaaa gtggcaatga cgctgactgg cgcgaaatcg
     6481 attagcgaaa ttacgcaaga ttcgctggtg caagggctgg gtagagaatt gcctgcggca
     6541 ctagctccga tggcgaaagg gaatgcggca tagttggggg tgaggggaaa aggccgtacc
     6601 aatcaccaat tttctgctat cctgcccccg cactaagggg gcagtatgct aaacatcgta
     6661 ctttacgaac cagaaattcc gccaaatact ggcaacatca tccgtctttg cgctaatacc
     6721 ggctttcgtc tgcatatcat cgaaccgatg ggatttgcct gggacgataa gcgcctgcgc
     6781 cgcgcggggc tggactatca cgagtttacc gccgttacgc gtcatcatga ctatcgcgcg
     6841 ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca ccacgaaagg tacgcctgct
     6901 cacagcgccg taagctatca ggatggcgat tatctgatgt tcggcccgga aacacgcggc
     6961 ctgccagcaa gcattcttga tgccctgccc gctgaacaaa aaattcgcat tccaatggtg
     7021 ccggacagcc gcagcatgaa cctgtccaat gcggtgtcgg tcgtggtgta tgaagcctgg
     7081 cggcagctgg gatatccggg agcggtgttg agagattagt tactgtatgc cggatagatg
     7141 attacatcgc atccggcaca atcacaggac gtcagatccc atccccatat tcaaacgtat
     7201 ggttaatacc gttgaaatgc tgatccatat ccattgacgg tttatcgctg tctggtttac
     7261 cgacaatgcg tgccggaacg ccagcggcgg tggtatgcgg cggcaccggt tgcagcacca
     7321 cggaacctgc gccaatcttc gcgccgcgcc caacttcaat attgccgaga attttcgcgc
     7381 ccgcgccaat catcacacct tcacgaattt tcggatgacg gtcgccacca gatttacccg
     7441 taccgccaag cgtcacagat tgcagaatcg atacgtcgtt ttcaatcacc gccgtttcac
     7501 ccacaacgat gcccgtggcg tggtcaagca tgataccgcg gccaattttt gctgccgggt
     7561 gaatatcaac ctggaacgtt acagaaacct ggttttgcag aaagattgcc agtgcgcggc
     7621 ggccctgatt ccacagccag tgaccgatac gataggcctg caaggcatga aaacctttca
     7681 gatataacaa tggagtcgag tatttatcca ccgccgggtc gcgggtacgc actgcctgaa
     7741 tatcacaggc cgcagaagcg atcatttccg ggtcggcggc gtaggcctct tccaccactt
     7801 cgcggatagc aatggcaggc ataattggcg atgacagctt gttcgccagc atgtagctca
     7861 gtgcactgcc caggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg gccagcattg
     7921 gctcacaatc cgccagcgtt ctggcttcgg ctttaatatt gttccagaca atttccagtt
     7981 cttcacacga cattgcttac tccacacgat gagataatga ccggcccgtt ctgcgcgggc
     8041 cgggtcatag cggtaacaaa ggtttcctgg gtttagtggc tgctgcgctc gtccttgcgt
     8101 gcacgaccta ataacgtcaa tgctgcctcg cgcgcgtttt ttccgcaata taatacttga
     8161 taaatttcct cggttattgg catttcaacg ccgaagcgat gcgccagttc gcggacttct
     8221 ttcgtattgc ggtagccttc caccacctga ccaatcttct cctgcgcgct ttgtacatcc
     8281 atgccctgac cgagcatcat gccaaaacgg cggttacgcg actggttgtc ggtacaggta
     8341 agcaccagat cgcccagccc cgccatgccc ataaaggtgg caggatcggc ccccagcgcc
     8401 gcgccaagac gcgacatctc agccagccca cgggtgatca gtgccgtacg cgcattcgca
     8461 ccaaaaccga taccgtcgga catccccgcg ccaatggcaa tgacgttttt caccgcgccg
     8521 ccaagctgca caccgatgaa atccgggttg ctgtaaacgc ggaaactttt gccacagtgc
     8581 aacaattgct ggagatcatc ggcaaaggtc tggtcggtag aggccagcga aatagccgtc
     8641 ggtaaacctg ctgccagttc tttcgcaaac gttgggccag agataaccgc cagcggaatt
     8701 tgatcgccta acgcttcacg cgccacgtcc tgtaacagac gcccggtttc cgcttccaga
     8761 cctttggtcg cccacaccag ccgcgcatca ggacgcatca gcggtttaat ctgacgcagc
     8821 acttcaccaa agacatggct gggtacgacg acgagaatat tacggctggc tgccagcgca
     8881 gtggcgagat cgctttcaag atggagcgta tcgggaaaag gcacatcggg gagaaacgcg
     8941 gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc ccagaggaca
     9001 acctcgtggc catttcttgc cagggtgatg gcaagagcgg tgccgtacga gccggcaccg
     9061 atcacagtca ttgaagcatt acgttggttc atcaggcatc ctgatgttct tcagtacctt
     9121 cgccagcctg ctgctgcaaa tagttcatga acagcgcatc gaagttaacc ggcgcaaggt
     9181 tcagttgcgg gaatgtaccg cgggatacca tgctggtgat gcactcacga gcatacggga
     9241 acagaatgtt cgggcagtat gcacccaggc aatgtgccat ctgggtgcct tcgatacccg
     9301 cgatggagaa aataccgccc tgctgaactt cacacaggaa cgcggtttct tcgcccaggg
     9361 aggccgttac ggtaacacgc agtaccactt cgtaaacgtc atctgccagt tgagaagatg
     9421 ccgtatccag atcaagttta acttctggct gccaatcttt ctggaaaacg tgcggcgcgt
     9481 tcggcgcttc gaaagaaata tccttggtat aaatacgttg gatctggaaa gtcatttcgg
     9541 tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa tactgcgcaa
     9601 tgtaatgcca acgttcacac gttatttcag caggggatcc agtccaccac gtgcatccaa
     9661 tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct ggggaaccgt
     9721 ggtgcgaccg ctgcgtttga tcatctcttc acgcttagcg gcgttgccat cgatcggcaa
     9781 ctcctgaaaa ctcacgccct tgctgctcag cagtgctttc gcacgatggc aatacgggca
     9841 ggtttctttg gtatagattt caacattggc cataacttag ctcctgtttt tttaccctga
     9901 aatttcttgt tgcagggcgg ctgcaaaatg gagtgtcccc agaagcttat aacaataagt
     9961 cgctgggggc ctaattgcag ctaacggcct gcatcatgaa agacgacagg taaattattt
    10021 gccgcgcacc agaggtaagt tttcgccagc ccagccagca acgccctctt tcagcacgaa
    10081 tacttgcgca aaaccagctt tcgtcagcgc gtttgcaggc tcctggcact gcatgccaga
    10141 accgtctacc acgataaccg gtttgtcttt gtgcttctca agctcaccaa cattgttggc
    10201 tttgatttcg cttggcaaca ggttgataga acctgcgata tggcctttac ggaagtcatc
    10261 acgctgacgt aaatccacaa ccacagcatc ttctttgttg atcagacgcg tagcttcacc
    10321 acgagtaatc accttcactt tcgaggtcag gctcttaaac gtagtcacaa gaaccgccac
    10381 cagtaacgcg atccaggcga tactcagtat gggatgacgg ccaacaaatt gcataatttc
    10441 ttgcatgggg ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa ggagtatacc
    10501 tgcgcggtgc ggcaaataca gccagcgcgt taactggaat gcaattttgc ggggcgcgac
    10561 gaaaaaaaag cgcaaatctg gcatcggctt gctgcgagcg gtcgtatatt ttgatcttca
    10621 gaggctattt tatcgattca gctgtagtaa aattacgcaa attttgactc ttgagtatga
    10681 ggttgtcgca atgtcggttt ctaaaaaacc tatggtactg gtgattctgg atggctatgg
    10741 ctatcgtgaa gaacagcagg ataacgccat ttttagtgct aaaaccccgg taatggatgc
    10801 actgtgggcc aatcgtccgc ataccctgat cgacgcttcc ggtctggaag tcggtctgcc
    10861 tgaccgtcag atgggtaact ccgaagtagg tcacgttaac ctgggtgccg gccgcatcgt
    10921 gtatcaggac ctgactcgtc tggacgttga aatcaaagat cgcgctttct tcgctaaccc
    10981 ggtactgact ggcgcggtag ataaagcgaa aaacgcaggc aaagcagttc acattatggg
    11041 tctgctgtcc gcaggcggcg tacacagcca cgaagatcac atcatggcga tggtagaact
    11101 ggcagctgaa cgcggcgcag aaaaaatcta cctgcacgca ttccttgacg gtcgcgacac
    11161 tccgccgcgc agtgctgaat cctcgctgaa aaaattcgaa gagaaatttg ccgcgctggg
    11221 taaaggccgc gtagcgtcca tcattggtcg ttactacgcc atggaccgcg ataaccgttg
    11281 ggatcgcgtt gaaaaagctt atgacctgct gactctggcg caaggtgagt tccaggccga
    11341 taccgccgtt gctggtttgc aggctgctta tgctcgcgac gaaaacgacg aattcgtgaa
    11401 agcgaccgtt atccgtgcag aaggtcagcc agacgcggca atggaagacg gcgatgcgct
    11461 gattttcatg aacttccgtg ctgaccgcgc acgtgaaatc actcgtgcat tcgtgaacgc
    11521 tgatttcgat ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga tgctgaccga
    11581 atacgccgct gacatcaaaa ctgcggttgc ttacccacca gcttccctgg ttaacacctt
    11641 cggcgagtgg atggcgaaaa acgacaaaac tcagttgcgt atttccgaaa ccgaaaaata
    11701 tgcccacgtt actttcttct tcaacggtgg cgtagaagag tcgttcaaag gcgaagatcg
    11761 cattctgatc aactcaccga aagtggctac ctacgatctg caaccggaaa tgagctccgc
    11821 agagctgacc gaaaaactgg ttgcggcaat caagagtggc aaatacgaca ccattatctg
    11881 taactatccg aacggcgaca tggtaggtca caccggggtg atggaagcgg cggttaaagc
    11941 ggttgaagcg ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt ccgtgggtgg
    12001 acaactgctg atcaccgctg accacggtaa cgctgaacag atgcgcgatc cggcaacggg
    12061 tcaggcacac acggcacaca ccaacctgcc agttccgctg atttacgttg gtgataagaa
    12121 cgtgaaagcg gttgaaggcg gcaaactttc tgacatcgcg ccgaccatgt tgtcgctgat
    12181 gggtatggaa atcccgcaag agatgactgg taagccgctg ttcatcgtgg aataatccct
    12241 ccccatgagg ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc gcaggtttgc
    12301 aatcaggccc atcatctacg ccagcgttct tagcgctggc gtattgttgt gcgccttttc
    12361 cgcccacgcg gatgagcgtg accaactcaa atctattcag gcagatatcg ccgcgaaaga
    12421 gcgcgcggta cgccaaaagc aacaacaacg ctccagcctg cttgctcagt tgaaaaagca
    12481 ggaagaagcg atctcggaag ccacccgtaa gctgcgcgaa acgcaaaaca cgctcaatca
    12541 actgaataaa cagattgatg agatgaacgc gtcgattgcc aaactggagc agcaaaaagc
    12601 cgcccaggag cgcagcctcg ccgcacaact ggatgccgca ttccgtcagg gcgagcatac
    12661 cggtattcag ctgattctca gcggtgaaga aagccagcgt ggacagcgtt tacaggctta
    12721 tttcggctat ctcaaccagg cgcgacaaga aaccattgct cagttgaaac aaacgcgtga
    12781 agaagtcgct atgcaacgcg ccgagctgga agagaaacag agcgagcaac aaacgctgtt
    12841 gtatgagcag cgcgcccaac aggcgaagct gacccaggcg ctgaacgaac gtaaaaagac
    12901 gctggcaggg ctggagtctt ccatccagca aggtcagcaa cagttgagcg agctgcgcgc
    12961 caacgaatcc cgtctgcgta acagcattgc ccgtgcggaa gccgcggcga aagcgcgtgc
    13021 tgaacgagaa gcgcgtgagg cccaggcggt tcgcgaccgc cagaaagaag cgacgcgcaa
    13081 aggcaccacc tacaaaccga ccgaaagcga aaaatcgctg atgtcccgaa ctggtggcct
    13141 gggtgcgccg cgtggtcagg cattctggcc agttcgcggg ccgacgctgc atcgctatgg
    13201 cgagcagcta cagggcgaat tacgctggaa aggaatggtt atcggtgcct ctgaaggtac
    13261 tgaagttaaa gcaattgccg acggtcgggt gattctggct gactggctgc aaggctacgg
    13321 tctggtggtg gtggttgagc atggtaaagg cgacatgagt ctttacggct ataatcagag
    13381 cgcactggtg agcgttggtt cgcaggttcg cgcgggccag ccgattgcgc tggtgggcag
    13441 cagtggcggt cagggtcggc cttcactcta tttcgaaatt cgccgccagg gtcaggcggt
    13501 caatccacag ccgtggttgg gaagataagt tttgtttcca tttcgtcgta acgttcttgc
    13561 atttgccgct ctgttggcgc tctcctcccc cgtaattgct ggcaaacttg ccatcgtcat
    13621 tgatgatttt gggtatcgcc cgcacaacga aaaccaggtg ctggcgatgc cttccgctat
    13681 ctccgtcgct gtattacccg attcaccaca cgccagagag atggcgacca aagcgcataa
    13741 cagcggtcac gaagtgttga ttcatctacc gatggcacca ctgagtaaac agccgctgga
    13801 gaaaaatacg ctacgcccgg agatgagcag cgacgaaatt gagcgcatta ttcgtagtgc
    13861 ggtcaataac gtgccctatg ccgtggggat caacaaccac atgggcagca agatgacctc
    13921 taacctgttt ggtatgcaga aagtgatgca ggcgctggag cgttacaatc tttacttcct
    13981 cgacagcgta accatcggta atacccaggc gatgcgcgca gcgcaaggca ctggcgtgaa
    14041 ggtgatcaaa cggaaagtgt tcctcgacga ttcgcaaaat gaagcggaca tccgtgtgca
    14101 gttcaatcgc gcaattgatc tggcgcgtcg caacggttca accattgcca ttgggcatcc
    14161 tcacccttca acggtacgcg tgctacaaca gatggtttat aacctgccgc cagacattac
    14221 gctggtgaaa gccagcagct tgctgaatga accgcaggtt gatacgtcta caccgccgaa
    14281 aaacactgtg cctgacacac cgcgtaatcc gttccgtggc gtgaagctgt gcaaaccgaa
    14341 gaaaccgata gaacctgttt atgctaatcg cttctttgaa gtattaagcg aaagcatcag
    14401 ccagagcacg ctgatcgttt acttccagca tcagtggcaa ggctggggca aacagcctga
    14461 agcggcgaag cttaacgcta gcgtaaatta aaggcgctac gcgtaatgcg atggtgcgac
    14521 cgcgttttgt cgcgccattg ccataagcgg aaagaccaca gtaacgcctg ataacccacc
    14581 ttcacgctgc gcacattggt aatcaggcgc ttatacatac cagaagtaaa tatctcggca
    14641 atcatccgtt ggcgggtaat aatatccggc tctttgcgca ccgcatggca aacacgcaat
    14701 gcttcgtaag ttatttgctg atgaaattcc ggataaatcg taattttgtc ggcataattt
    14761 cgatttaatt tctctagcag gcgggtaatt ttaatatagt gacgttgata attaaggttt
    14821 ttattccctt gtctattcaa ccgactcact gacgtattat gcagataata tttatataac
    14881 gattgctcgg tatatcgcgc acgcagcgca ttaaacataa attctgttgt ccagacaata
    14941 tcctgatgat gtaatccggc aataaattta atgttatttt taacaataac atcgcggcga
    15001 taaaccccca tccagacaac gtgagtccaa cggcgggaag aaagccccat ccgcaaccaa
    15061 tccgggccgg ttaatacgcc ggttgagcga aggcgatcgg tggggatgga ttgccaggtt
    15121 tctcccgttt cacgaaaaca ccagtcagcg ttgcactgcg ccacgtcgag gtcgtcctct
    15181 aacgccatgg tcatcagcgt ttcgtacatg gtgggataca cctcatcgtc agcatcgaca
    15241 aaagcgacat atttgcccgt cgccacctcg atcccacgat tacgcgccac cgatgcgcca
    15301 gcattcgcct gatgcaacaa acgaacgtgc ggatagtttt ctgcgtaatg ctttgctatt
    15361 tcaacagaat tatccgttga accatcgtta ataataatga tttccagagc agtccaggtt
    15421 tgcgtaatta aagattccat acaagtgcgg aaatcatcgc ctgcattata taacggaata
    15481 ataacactaa gtttattggt gctgttcatc ataaattacc gacgacctct gaagttatcc
    15541 tgacgtttta caggctttgt attaagaaac tattaagcct gtgagatatt atttttacag
    15601 gtgagagagt cacttcaggg tagagagata atgagaacag ctatataaga gatcagcgca
    15661 attacccatt ctttatttta ctgacagcat tttacatatt taacagatta tcctcaggaa
    15721 aaagggctgg aataccagcc cttgtttgtg ttaatcccag ctcaggataa ctttcccgga
    15781 ctggcccgaa cgcatggcgt caaagccctt ctggaaatca tcgatagaga aacgatgggt
    15841 aatgatcggc gaaagatcga ggccagactg aatcagcgcc gccatcttgt accaggtttc
    15901 aaacatctca cgaccgtaaa tacctttaat gaacaatcct ttaaagatca ctttggtcca
    15961 gtcgatagac atatcagacg gtggaatacc cagcatcgca atacgaccgc cgtggttcat
    16021 ggtgtcgagc atggtacgaa acgctggcgg cgcaccggac atttccagac cgacatcaaa
    16081 gccttcggtc atgcccagtt ctgccatcac atcattgagg ttctctttag cgacgttaac
    16141 cgcacgggtg atacccattt tacgcgccaa ctcaaggcgg tattcgttaa catcagtaat
    16201 caccacgtta cgtgcaccaa cgtgtttcgc caccgccgcc gccataatac caatcgggcc
    16261 tgcaccagag accagcacat cctcgcccac cagatcaaac gacagcgcgg tatgcacggc
    16321 gttaccgaag ggatcaaaaa tggaagccag atcatcggag atattgtcag ggattttgaa
    16381 ggcgttgaac gccggaatca ccagatactc ggcgaagcag cccggacggt taacaccgac
    16441 gccgatcgtg ttgcggcaca gatgggtacg accgccacga cagttgcgac agtgaccgca
    16501 ggtgatatgg ccttcgccag aaacgcgatc gccaatcttg aagcctttca cttcctgacc
    16561 aatacctacc acttcaccga catattcatg gcccacgacc atcggcaccg ggatagtttt
    16621 ttgcgaccac tcatcccagt tatagatgtg aacgtcagtc ccgcagatgg ctgttttacg
    16681 gattttaatc agcagatcgt tatgcccgag ttccggtaca ggaacgtcgg tcatccagat
    16741 gccctcttcc gctttcagtt tggataacgc tttcatctca catcctcagg cgataacgcc
    16801 cagttgttta ccaatacgcg tgaatgcttc tactgcacgc gtaatttgct caggggtatg
    16861 cgccgcagac atctgggtac gaatacgcgc ctgacctttc ggaacgaccg gatagaagaa
    16921 accggtgacg taaatcccct ctttttgcag ctcacgagca aatttctgcg ctactaccgc
    16981 atcgccaagc atgaccggaa taatggcgtg atcggctccc gccagggtaa agcccgccgc
    17041 cgacatttgc tcacggaact gacgcgcgtt cgcccacaga cggtcacgca gctcgctacc
    17101 cgcttcgacc atctccagca ctttgataga cgcggcaaca atggccggtg ccagcgagtt
    17161 ggagaacagg tacggacgag aacgctggcg cagccactca accacttctt tgcgcgccgc
    17221 cgtgtagccg ccagaagcgc cacccagcgc tttaccgagc gtgccggtga taatatcgac
    17281 ccggcccatc acatcgcagt attcatggga accacgaccg ttttcaccga caaaaccgac
    17341 cgcgtgggag tcatctacca tcaccagggc atcgtattta tctgcgagat cgcaaacacc
    17401 tttcaggttg gcaatcacgc cgtccatcga gaacacacca tcggtggcaa tcagcacatg
    17461 acgcgcaccg gcttcacgcg cttctttcag acgtgcttcc agctcctgca tatcgttgtt
    17521 ggcataacga tagcgtttag ctttgcacag acgcacacca tcaataatag acgcgtggtt
    17581 cagtgcgtcg gagataatgg cgtcttccgc gcctaacagc gtttcgaaca ggccaccgtt
    17641 agcatcaaag caggaagagt agagaatcgc atcttccatc cccaggaagg ccgccagttt
    17701 ttgttcaagc tctttatggc tgtcctgagt gccgcaaata aaacgcaccg aagccatgcc
    17761 gaaaccgtga gaatccattc ccgcctttgc cgccgcaatc agatccggat gattcgccag
    17821 tccgagatag ttattggcac aaaagttaat gacgtggctt ccatcagcca cagtgatatc
    17881 tgcttgctgc gcagacgtaa taatgcgctc ttctttaaac aacccttccg cccgtgcggt
    17941 ttccagatcg ttggttaact gctgataaaa ttctccacgc attgcgattc tccagacagg
    18001 gcaaattcca gcacatatta cccaaactta taggtcgcga cgagatgacg cgttaacact
    18061 tctgcaaaat tcaggataaa taacgtccat caatctcact tttggcgaat ggctgaagcc
    18121 aacgccgtgt agtgatatga taggagtatt cgtgtctgag attgtctctg actccataat
    18181 tcgaaggtta cagttatgat catcgttacc ggcggcgcgg gctttatcgg cagcaacatc
    18241 gttaaggccc tgaatgataa aggcatcacc gatattctgg tggtggacaa cctgaaagac
    18301 ggcaccaagt ttgtgaacct ggtggatctg gatatcgcgg actatatgga taaggaagac
    18361 ttcctgatcc agattatggc tggcgaagag ttcggtgatg tcgaagcgat tttccacgaa
    18421 ggcgcatgct cttccaccac cgagtgggat ggcaagtata tgatggataa caactatcaa
    18481 tactccaaag agctgctgca ctactgtctg gagcgcgaaa tcccgttcct gtatgcctct
    18541 tccgcagcca cctacggcgg acgcacctcc gactttattg aatcccgcga gtacgaaaaa
    18601 ccgttgaatg tctacggtta ctcaaaattc ctgtttgatg aatatgttcg tcaaatcctg
    18661 ccagaagcga actcgcagat tgttggcttc cgctatttca acgtttatgg accgcgtgaa
    18721 ggccataaag gcagtatggc gagcgtcgct ttccatctca acacccagct taataacggt
    18781 gaatcgccga agctgttcga aggtagcgag aacttcaaac gcgacttcgt ttacgtaggc
    18841 gacgtggcag atgtaaacct gtggttcctg gaaaatggcg tttccggcat cttcaacctc
    18901 ggtactggtc gtgcggaatc cttccaggct gtagcagatg ctacgcttgc ttatcacaag
    18961 aaaggccaaa tcgaatacat tcctttcccg gataaactga aaggccgcta tcaggcgttc
    19021 actcaggcag atctgacaaa tctgcgcgcg gcgggttacg acaaaccgtt caaaaccgtt
    19081 gccgaaggcg taacggaata catggcctgg ctgaatcgcg acgcataaga gctctgcatg
    19141 aaaatactgg tgatcggccc gtcttgggtt ggcgacatga tgatgtcgca aagtctctat
    19201 cgcacgctcc aggcgcgcta tccccaggcg ataatcgacg tgatggcacc ggcatggtgc
    19261 cgtccattat tatcgcggat gccggaagtt aacgaagcta ttcctatgcc tctcggtcac
    19321 ggagcgctgg aaatcggcga acgccgcaaa ctgggtcata gtctgcgtga aaagcgctac
    19381 gaccgcgcct acgtcttacc caactccttc aaatccgcat tagtgccttt cttcgcgggt
    19441 attcctcatc gcaccggctg gcgcggcgag atgcgctacg gtttacttaa cgatgtacgc
    19501 gtgctcgata aagaagcctg gccgctaatg gtggaacgct atgtcgcgct ggcctatgac
    19561 aaaggcatta tgcgtacagc acaagatctg ccgcagccat tgttatggcc gcagttgcag
    19621 gtgagcgaag gtgaaaaatc atatacctgt aatcaatttt cgctttcatc agaacgtccg
    19681 atgattggct tttgcccggg tgcggagttt ggtccggcaa aacgctggcc acactaccac
    19741 tatgcggagc tggcaaagca gctgattgat gaaggttatc aggtggttct atttggctcg
    19801 gcgaaagatc atgaagcggg caatgagatt cttgccgctt tgaataccga gcagcaggca
    19861 tggtgccgga acctggcggg ggaaacacag cttgatcaag cggttatcct gattgcagcc
    19921 tgtaaagcca ttgttactaa cgattctggt ctgatgcacg ttgcggcggc gctcaatcgt
    19981 ccgctggttg ccctgtatgg tccgagtagc ccggacttca caccaccgct atcccataaa
    20041 gcgcgcgtga tccgtctgat taccggctat cacaaagtgc gtaaaggtga cgctgcggag
    20101 ggttatcacc agagcttgat cgacattact ccccagcgcg tactggaaga actcaacgcg
    20161 ctattgttac aagaggaagc ctgacggatg cgggttttga tcgttaaaac atcgtcgatg
    20221 ggcgatgttc tccatacgct gcccgcactc actgatgccc agcaggcaat cccagggatt
    20281 aagtttgact gggtggtgga agaagggttc gcacagattc cttcctggca cgccgccgtt
    20341 gagcgagtta ttcctgtggc aatacgtcgc tggcgtaaag cctggttctc ggcccccata
    20401 aaagctgaac gcaaagcgtt tcgtgaagcg ctacaagcag agaactatga cgcagttatc
    20461 gacgctcagg ggctggtaaa aagcgcggcg ctggtgacgc gtctggcgca tggcgtaaag
    20521 catggcatgg actggcaaac cgttcgagaa ccgttagcca gcctgtttta caatcgtaag
    20581 catcatattg caaaacagca gcacgccgta gaacgcaccc gcgaactgtt tgccaaaagt
    20641 ttgggctata gcaaaccgca aacccagggt gattatgcta tcgcacaaca tttcctgacg
    20701 aacctgccta cagatgctgg cgaatatgcc gtatttcttc atgcgacgac ccgtgatgat
    20761 aaacactggc cggaagaaca ctggcgagaa ttgattggtt tactggctga ttcaggaata
    20821 cggattaaac ttccgtgggg cgcgccgcat gaggaagaac gggcgaaacg actggcggaa
    20881 ggatttgctt atgttgaagt attgccgaag atgagtctgg aaggcgttgc ccgcgtactg
    20941 gccggggcta aatttgtagt gtcggtggat acggggttaa gccatttaac ggcggcactg
    21001 gatagaccga atatcacggt ttatggacca accgatccgg gattaattgg cggatatggg
    21061 aagaatcagg tggtttgtag ggctccgcta atgaatttaa ataatttaga agcagcggct
    21121 gtttataaaa aaatcacatt aatttaataa atagtaagtg ggcattatac ccacttactt
    21181 agtcaacgct tttattattg tggttcattt ttactcaaaa acgatataga caggaagagg
    21241 cacacaatcc acgatgccgg tattggttta gcatgtgtaa tgacatcact aatgccaaat
    21301 aacataatag aagcagggaa aaccaagagt atatattttt tctctcgtat acagtaatag
    21361 atcattgcaa catagaacat aataagaaca agaagaccaa tttttccttt cgtggaaaga
    21421 gattccacaa tttcgttatg catatgtaca tcaaggaata ataaagctcc actataaata
    21481 ttattttctg cacttaatgc ttttatcttt tcagcacgct gctcaagaga ttgccaatta
    21541 aaaccatcag gacttccttt aattcctgca ttaaccattg caaaacgtgc tccgaccgag
    21601 gattgactgt tattttttgt agcatatgaa ataacatcag cttttaatga attaattcgc
    21661 ctatcaattt ccttattaaa tatcaaacca caagcaagta gcaatataaa aacaccactc
    21721 aatcctttaa ataaaaacac tttttgatca cgatgctgaa gaaataatat gaccataatc
    21781 ataagtggga atgtgaaaat tgccgcacgc gttcctgtca tcattactgc tgcaaagctt
    21841 aaagcaaagt tagcataaaa tagaaaccat ttatatttcg attgcatttt tatgaataca
    21901 attgaagaat ataaagttgt aaatgtaatc acataagccg caatagtcgc tgcccccatc
    21961 tgaccaatat tcccattcat ggcggagagg ggaatgcggt ctgcatgcaa atatatccct
    22021 tgataatatg ctctacatag catgattatt tgaactaaga tagctgaaat aaaatgtagt
    22081 ttttcatttt gcctgaattt atttttactt agtaataaaa atgtcaacga gctaaatact
    22141 aatagttttc cagactcaag gtagcctcga taagcatttc tatatactac atcttctgtt
    22201 ttataaaatt cataccatat aaggttcatt gcacccaaaa gaagtaaacc aagaattaat
    22261 ataatgacat ttttattata attgtgcttt ttatttacaa ttaataagaa aagagaaaat
    22321 gcacctgtcg cattaattat tttagtggtg atattaagat caattggcac aaacaacaaa
    22381 ccgattaaat acaataaaaa aataaaatta cagctattat ttcgaaattg ctcaattttt
    22441 acttccacgt taatatctca caacaaattg aaattaacaa tctcatttca aaagagaaaa
    22501 aaaagccacc tttatccagg agcgtataaa cttcattctt ttctgaagcc ttgttctagg
    22561 ttgttttttt ccgatgttaa tagcctttca ctttcaagat gactattaaa ctcatttaaa
    22621 ataggatgta atattatttc gtctaaataa aactctttta taatattatg ttgatgcatt
    22681 aacttccagc tatcagctcg ataggtgcca taatcctttg tcaatttaag caacttttgt
    22741 gccatatctt tattcatcaa atatgaacat gtacgacgaa ttttattttt attgaaatta
    22801 acacgccgaa aagtacaagt acttacctta actttactaa aaaggctttg ccccaatacc
    22861 ggataatcat gtaatccttt ctgtcctcca agcaaataca aattattatg ttttaacttg
    22921 tctttttcag gaagattaaa atattgcaga aattttttaa atttctcatt gacgataaca
    22981 tcatcctcga gaatgactgc ccattctata tttttatcta tcatatcctt atatataagc
    23041 tgatgactta atgtacaagc aatttctcca tcagtcattt ctgcgccgac accacttaat
    23101 ctcattttat caataatttc tttattttga gggtctttag catctatggc atcaaaaaaa
    23161 tcaaaattaa tatttaatcg atgaaaaaca tcatttattt tatttcttcg ctcaatatct
    23221 cttttaagtg agacaatata aatgggcaag ttcattaaaa aatctctggg tgattaatct
    23281 taataattat ttggaaaaat gcttacgcgc tattttctta ctataataga gcatgaaata
    23341 gtaaaatgaa gatgcaatat taccatttct tttcaaatgt ctggatttca cttgatactg
    23401 cttttcattc gtggctggaa taaacgccac atcattccag caagaagcct gataagcctc
    23461 attaaaatac ttctggcagg gataattcgt ccagtcgttc cagggcttcg ttacgccaac
    23521 ataatgaatc agcttagtat catcggtaat ggtcttttta taatcttcat cgcttttatt
    23581 ttttaactca tagtcaatgc cataaaagca atcataatcg ccgctaataa aaatacaatg
    23641 accaaagaac agaatattta aaatatcctg atcaaggcat ttatacttcg tatgctgcgg
    23701 gtcacttgcc agcatagcaa tggctttatt cataaaatca tttttcgccc atgcttcaag
    23761 ctgcagatat actacaccgg cgttgaaata attacctgga agtccctcta tagccagacg
    23821 cttcgctggt ttttcctgca taaaaggcac atctttaata acagctgcga acttatcgcc
    23881 atcaaaatta atatcaagca atccagctaa tgagccttta cagatgacat cagcatcgat
    23941 atataacaga ttttcaataa cttcactcag atactggaac gcaactaaac ggaagaatgt
    24001 tgcataagac cactgtttca ggctggggaa gatatcaata aaggcactgg agactttata
    24061 aattcttact tcaacgttag catttttgct ggtaaaagcg ttaacacgtt gtagataatc
    24121 ttcatcaatg tagtcggtaa agagatgaaa cttaaccgca aggtccgggt tatgcataat
    24181 aactgattgc atagatactg ccgcaccaaa taaaaagttg cggtcaatac cgtaagcgat
    24241 atttaacaca cgatcggtgt tagtaatttt cccagagaca gtgacttcag taatactctc
    24301 agctaataaa tccatgttgg ttccgttttg actgtgtggt ttacgctttg ccttttaatt
    24361 ttttaataaa tttgcgtagt ttggttctgt agattactga atagtagcca taatctttta
    24421 tttcgttggc aatacccagg tgacgttcca tggccagacg atcacgagct ttacgctctg
    24481 ccgtacagct tttaccggat aggtcaatta tcctgacacc atctttacta acgataaagt
    24541 taccccggtg tgggtcgccc gatagcatat ttaaggcatg caatttttcc attgaagcct
    24601 taatttctgc tttaacattt tctggaataa tcggcatatc attgagctct acgccctcga
    24661 catactcgat aagcatgata aaaacactgg catagttgaa aaatttacgc tcagccagaa
    24721 aataaaaatc attaggaaaa gtaagacctg cacttctgac acgatcggtt tcaacgatca
    24781 aattctgata gtagtcacct tttacaaaag atttaaaaaa acgttcattc cttttcgttt
    24841 tcggagcaaa gactttaaaa acatagcgac catatttcgt atcaatcaac gatactttgg
    24901 tatcatcaat attacgtaat acttttactg ttttaaaatt ataggccagt atttcatcaa
    24961 agatttcttt atatttatta tcattatctt tgtaataaaa tgagaagccg cgataacgta
    25021 tacttgtaat cataagagta atttatttct taagcttgta cttaattaat gaagttatgc
    25081 cttttatata ctcaccatgg gcaaacagat gcttatattg tttctgcatt tctgcaacag
    25141 tccgcgcctc tttaagagga tatttcttcc agggagattg ttcacgcgcg atattaaagt
    25201 atgatgcaga cggatatcca gcccagctat gccatggttt agttatccct gtatagtgaa
    25261 tcaacactgt tttatcggta atggtttgct gatactttcg atgactacga tcataaagtt
    25321 cgtttttcag ggtataaata gtatcaaaat ccttcgcaag gtagatatta ttcatattaa
    25381 atgcgatatt caacgcatcc tgatctaaat atttaagaga gccatattta gtttcccctc
    25441 gtaaaagttt gagtaaatac ggtgtcaaat ttgcttcatg ccatttttta agattgacat
    25501 agataacgcc tgcattgaaa taacgtccat tcatttcggg aatgttgagg cgttttgcac
    25561 atgccgcctg agtactatca ttgtcaggaa tgaccgcagc aaactcatct ttaaatataa
    25621 tttctgtgag aggtttcagg cttcctttac aaacaacatc ggcatccaga tacagcagtg
    25681 tggaaatact ttcactcaga tattcaaaag acaataccct gaagtatgtc gcgtacgacc
    25741 aaaactgtga cgtcggcaga tcagcaaagt atttggggtc aattaaatat acaataatt
//
LOCUS       DAPOZO010000069        25159 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPOZO010000069 DAPOZO010000000
VERSION     DAPOZO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.069"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(13..342)
                     /locus_tag="ROI21_003113"
     CDS             complement(13..342)
                     /locus_tag="ROI21_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDX5112006.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(377..1837)
                     /gene="cls"
                     /locus_tag="ROI21_003114"
     CDS             complement(377..1837)
                     /gene="cls"
                     /locus_tag="ROI21_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDX5112007.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            1980..2153
                     /locus_tag="ROI21_003115"
     CDS             1980..2153
                     /locus_tag="ROI21_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDX5112008.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(2208..3461)
                     /gene="kch"
                     /locus_tag="ROI21_003116"
     CDS             complement(2208..3461)
                     /gene="kch"
                     /locus_tag="ROI21_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDX5112009.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(3762..4058)
                     /locus_tag="ROI21_003117"
     CDS             complement(3762..4058)
                     /locus_tag="ROI21_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDX5112010.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            4282..5007
                     /gene="tonB"
                     /locus_tag="ROI21_003118"
     CDS             4282..5007
                     /gene="tonB"
                     /locus_tag="ROI21_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDX5112011.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     KPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSR
                     NQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYE
                     PGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(5047..5445)
                     /gene="yciA"
                     /locus_tag="ROI21_003119"
     CDS             complement(5047..5445)
                     /gene="yciA"
                     /locus_tag="ROI21_003119"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDX5112012.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(5550..6089)
                     /locus_tag="ROI21_003120"
     CDS             complement(5550..6089)
                     /locus_tag="ROI21_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDX5112013.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(6119..6862)
                     /locus_tag="ROI21_003121"
     CDS             complement(6119..6862)
                     /locus_tag="ROI21_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDX5112014.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(6890..6979)
                     /locus_tag="ROI21_003122"
                     /pseudo
     CDS             complement(6890..6979)
                     /locus_tag="ROI21_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            7219..7857
                     /gene="ompW"
                     /locus_tag="ROI21_003123"
     CDS             7219..7857
                     /gene="ompW"
                     /locus_tag="ROI21_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDX5112015.1"
                     /translation="MKKLTVAALAVTTLLSGSAYAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(7916..8422)
                     /locus_tag="ROI21_003124"
     CDS             complement(7916..8422)
                     /locus_tag="ROI21_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDX5112016.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKHMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(8468..8968)
                     /locus_tag="ROI21_003125"
     CDS             complement(8468..8968)
                     /locus_tag="ROI21_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDX5112017.1"
                     /translation="MNMKTIEDVFIHLLSDTYTAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(9054..9233)
                     /locus_tag="ROI21_003126"
     CDS             complement(9054..9233)
                     /locus_tag="ROI21_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDX5112018.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(9614..10420)
                     /gene="trpA"
                     /locus_tag="ROI21_003127"
     CDS             complement(9614..10420)
                     /gene="trpA"
                     /locus_tag="ROI21_003127"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDX5112019.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(10420..11613)
                     /gene="trpB"
                     /locus_tag="ROI21_003128"
     CDS             complement(10420..11613)
                     /gene="trpB"
                     /locus_tag="ROI21_003128"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDX5112020.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(11625..12986)
                     /gene="trpCF"
                     /locus_tag="ROI21_003129"
     CDS             complement(11625..12986)
                     /gene="trpCF"
                     /locus_tag="ROI21_003129"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDX5112021.1"
                     /translation="MMQTVLAKIVADKAIWVETRKEQQPLASFQNVVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVMVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLRGALPENVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(12987..14582)
                     /gene="trpD"
                     /locus_tag="ROI21_003130"
     CDS             complement(12987..14582)
                     /gene="trpD"
                     /locus_tag="ROI21_003130"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDX5112022.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(14582..16144)
                     /gene="trpE"
                     /locus_tag="ROI21_003131"
     CDS             complement(14582..16144)
                     /gene="trpE"
                     /locus_tag="ROI21_003131"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDX5112023.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMHCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAGAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(16166..16259)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(16236..16280)
                     /gene="trpL"
                     /locus_tag="ROI21_003132"
     CDS             complement(16236..16280)
                     /gene="trpL"
                     /locus_tag="ROI21_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDX5112024.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            16418..17299
                     /gene="yciV"
                     /locus_tag="ROI21_003133"
     CDS             16418..17299
                     /gene="yciV"
                     /locus_tag="ROI21_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDX5112025.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            17296..17916
                     /locus_tag="ROI21_003134"
     CDS             17296..17916
                     /locus_tag="ROI21_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDX5112026.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            17944..19827
                     /locus_tag="ROI21_003135"
     CDS             17944..19827
                     /locus_tag="ROI21_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HDX5112027.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYPKNTEIPFYEEGISLFDVEATL
                     QPDGGLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGVPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQQFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVVLTGYLTGI
                     GYIFLPKFTQTGQQRYAHGEAIVNYLAGKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQTHLSCGAYTSLLGRSYSGGGAGGGGG
                     GGW"
     gene            20040..20915
                     /gene="rluB"
                     /locus_tag="ROI21_003136"
     CDS             20040..20915
                     /gene="rluB"
                     /locus_tag="ROI21_003136"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDX5112028.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDTKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            21085..22086
                     /locus_tag="ROI21_003137"
     CDS             21085..22086
                     /locus_tag="ROI21_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112029.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     FKQYYASRENFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFE
                     KEKLLASMEVINSMSAYVELLTKDMATTESQQVALLKQALDHANNAQTLLGVSHSKDL
                     AQSAAAVKDLVNYFNELYIIKVNSGKIGKTADENWVRLIKNMAVLRNDIYTKSNKIEN
                     YTFANVHDNMFYYNTVSKNSKNTEFNTLKNRKEFLSSVINVNETRLQASAPAMPAIEA
                     LNAFIDKGDYLKSLYVNKTRTAAEQKRLNEILRAQVISGLKASEGLAKLLIASGIML"
     gene            22097..22405
                     /locus_tag="ROI21_003138"
     CDS             22097..22405
                     /locus_tag="ROI21_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112030.1"
                     /translation="MNDVQNRRILALLDMLYNTLGNKTTCNVCHKYFIHILTVMNNLT
                     INYGTETSPELARINYDDANALITELILFIEDVDAENHEAYPGHLNEITRILNFAIAA
                     "
     gene            complement(22457..23047)
                     /gene="cobO"
                     /locus_tag="ROI21_003139"
     CDS             complement(22457..23047)
                     /gene="cobO"
                     /locus_tag="ROI21_003139"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDX5112031.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVKALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(23044..23802)
                     /locus_tag="ROI21_003140"
     CDS             complement(23044..23802)
                     /locus_tag="ROI21_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDX5112032.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            24022..25071
                     /gene="sohB"
                     /locus_tag="ROI21_003141"
     CDS             24022..25071
                     /gene="sohB"
                     /locus_tag="ROI21_003141"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDX5112033.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNISEQYKEMKEELAAALMDTHQQKQWHKTQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
BASE COUNT         6520 a         6053 c         6280 g         6306 t
ORIGIN      
        1 gaaagcgatt atttattccc gccagatgat atggcacagt ttgtgatctt tttcgcggca
       61 taacagaatg cgggcgaaaa catcgttgat ttcaccatct tcactgtccg ccagaccaat
      121 caccacttcg gcaaaaaagt ccgggttcaa atcgaaatca acatgttcct gccagtcttc
      181 tgccggatcg aataactccg cgccgccgcg ctcttcaaac tgaagattga acagcagaac
      241 atcagctgga tcgagattgt cggcagccag ttcgagaaaa atatcgtagg cctgctcaag
      301 cgtttcatct tcagtcaggc gattgtttag atccatatcc atagtcacta cctgtttaac
      361 ctctgttggc gacgttttac agcaacgggc tgaagaagta aaacagtcgc tcggcgacac
      421 gttgccataa tggacgtttt agccataaac gggcatcgag cagacgagaa cgcgaaatat
      481 aatcgtcctg aacggcggcg aggtcagcac caaacccttt atcgtcgatt gccagcgtaa
      541 tctcgaaatt cagccacaga ctccgcatat caaggttaac ggtaccaacc agacttaatt
      601 cgccatcgac cagcacactc ttggtatgca ataacccgcc ttcaaactga taaattttaa
      661 ccccagcagc cagcagttcc gtaaagaacg cgcgactggc ccagccaacc agcattgaat
      721 cattttttcg cggaaggata atactgacat ccactccgcg ctgtgccgcc gtgcaaatcg
      781 catgaagtaa atcatcgctg ggcacaaagt agggcgttgt catgatcaaa tattcacgtg
      841 ccgaataagc cgcagtcaat aatgcctggt gaatgagatc ttccggaaag ccggggccag
      901 aagcaattgt gtgaatggtg tgaccgctgg cctgctcaaa cggcataata ttgacatctg
      961 gtggtggcgg cagaatacgt ttcccggttt caatctccca gtcgcaggaa taaataatcc
     1021 ccatcgcggt ggcgatgggg ccttccatac gcgccatcag atcaatccat tgccctacgc
     1081 ccgcatcttg tttgaagtag cgagggtcga ccatattcat gctgccggtg tacgcgatgt
     1141 aattatcgat catgatcatc ttgcgatgtt ggcgcaggtc catacggcgt aaaaacacac
     1201 gcatcagatt gacctttaag gcttcgacca cttcaatacc ggcattacgc attagctcgg
     1261 gccacgggct gcggaaaaaa gccacactcc cggcggagtc gagcatcaat cggcaatgaa
     1321 tgccgcgtcg cgcagccgcc attaatgatt cagccacctg gtccgccatg ccgccgggct
     1381 gccagatata aaacaccatc tcaatattat ggcgcgcgag ctggatgtcg cggattaacg
     1441 cctgcatcac atcatctgac tcggtcatca gttgtagctg attccctttg accccagcga
     1501 tcccctgacg acgctcacaa agcttgaata gtggcgcagc gacactgcta ttttcttcgg
     1561 cgaagatatg cttacaggct ttaaggtcgt taagccactt tgcggtggaa ggccacatcg
     1621 ctctggcgcg ctcagcgcgg cgtttgccta aatggagctc gccaacggca agataggcaa
     1681 taattccgac taacggcaga atgtaaataa tcaacagcca ggccatcgcg gagggaactg
     1741 cgcgtcgttt cattagaatg cgtaaagtta cgcctgcaat gagcaaccag tatcccagaa
     1801 tggccaacca actcaccaac gtataaacgg ttgtcataga ttagaaatcc ttttgaaagc
     1861 gcattgttat gagtttacgc atatggattc atctggcaaa taaaaacgcg ggaaaagcac
     1921 tggtcagcgt cgggggcggg ggtataatga ccattctgtt attgcataga gtagttaaca
     1981 tgaagcggag tagaacggaa gtggggcgct ggcgcatgca gcgtcaggct agccgacgta
     2041 aatcgcgttg gcttgagggg caatcgcgcc gaaatatgcg tatccacagc atcaggaagt
     2101 gcattctaaa caaacagcgt aactcgttat tgtttgcgat ctacaatatc taaatgtact
     2161 gggcaccatt gcggtgccca ataagtttac tcttccgtct ttaaactcta tttttgcgcc
     2221 gattctttac tgtcagcttt cgtttcttgt tcatcgttat cgctgaaaat gccatgaccg
     2281 gagttcaaca acattgaaac gagcatatca ttattaatct cttcaccatt taaaactcgc
     2341 gccagaattt cgctgccaaa cagttgcggc gagagaatga tatccggatg taccatctta
     2401 atcttattca ggtttttgct atcactgacg gcgagaactg ttttgacatc actgctcata
     2461 tcttttgccg agagtacaac aaacgcgtta tctgcatcgt tatcactcag cgccagaatg
     2521 gcccggcaac gatcgattcc cgctttcttc aagactgaac tgtcattact gtcaccgggg
     2581 ataacatcag cgttatcgcc taagcgttgc tcaagttgct tgatatcatc ttcaggcaag
     2641 ttgctgataa ccgttacgtt ttgtccgcgt tgattcagtt gcaggatcgt attgatggcg
     2701 agaatcgaat gtccgcaaac aataaaatga tctttacgat gcattgtatg attgtttcct
     2761 tttacaagtt tgttgaatcc cccgcggata agcggaccaa aaattgaggt catagaggtg
     2821 gcaaaaacgg taatgccgga aataatgacc gaaatagtga acaatcgtgc tgattcagaa
     2881 acagggacaa tatcgccata gccgacggtt gacatggttt ctatcgaaaa atagaacgcg
     2941 gtcatcaaac tttctattcg cggattaaaa ccttcgctta aataaagcgc accgtaggtg
     3001 gagtaaaaca gtaacgtcgt gaaactaata aatgcgaaaa ttgtcccggc tgcggcgcta
     3061 ctgtgggaga agtctttgcg cagtatcagc aaaaacacca gcgtaaaaat gcaaaatcca
     3121 atactaaatt tcagccaggg ataaaaatgc agggtgtaaa ttagcgctat caacaacaaa
     3181 atgatactga ttgcccatgc gagctttgcc cggaacagta agccaatgga gttcaggaca
     3241 agaaagacgc cgagcataaa gagtggggcg tttgccagca aggagagatc caactctgaa
     3301 aatgctttga tatggaaaat atctagcaaa tttaccgaca taccatagat tgttttaaaa
     3361 ataagcaatc catttaaaaa gacggccagc gcgagaatgt cgtgccgtag ggtcacccat
     3421 aaattagttg ctgtttgttt gaatgtagcc cagtgactca ctgatacctc tgtaaccaca
     3481 attttgttta attatctgag cgtgcacatt ttctgaacat acatgcagcg cgtgaatgtg
     3541 ttaaacaata gtagatgact ttatccgcag taatcaaagg ttttgagtga gcaactctgc
     3601 gtcaggaaag ggcatttttc ctgaatatgc tcacatcata taaagaaata cagataaagt
     3661 tattatctgc ttgtgggggt gaatgcactg accgacctat aaggaaaggc caaacaagaa
     3721 cacggttgca aaaaccgtgc ccttaaatat tgaatctcta ttcagaacac tttcttaaat
     3781 ggtttcactg aaacgtgttc atagactcct gccgctacgt acgggtcagc atcggcccag
     3841 gcctgagctg cttccagcga ttcaaattca gcaataacgg ttgagccagt aaatcccgca
     3901 gcccctggat cgttactgtc taccgctggc attggaccag ctgtcaacaa acgaccttca
     3961 tcatgcagta actgtaaacg tgctaaatgt gccggacgaa cggaaaggcg tttttcgagg
     4021 ctatcagctt tgtcttgagc gtaaataaca tacaacacgg gcacaactcc ttgttcggga
     4081 aagttgtaaa gtacgttatt tgaaagggcg aagatctgca acggaaagat gatgtctttg
     4141 ttaagaccat gcataaagta aggtcaatta cgccaaaaat gacattttca ctgatcctga
     4201 tcgtcttgcc ttattgaata tgattgctat ttgcatttaa aatcgaggcc tggtttttct
     4261 actgaaatga ttatgacttc aatgaccctt gatttacctc gccgcttccc ctggccgacg
     4321 ttactttcgg tctgcattca tggtgctgtt gtggcgggtc tgctctatac ctcggtacat
     4381 caggttattg aactacctgc gcctgcgcag ccaatttctg tcacgatggt tgcgcctgcg
     4441 gatctcgaac cgccacaagc cgttcagccg ccaccggagc cggtggtaga gccagaaccg
     4501 gaacctgagc cgatccccga accgccaaaa gaagcaccgg tggtcattga aaagccgaag
     4561 ccgaagccga aacctaagcc aaaaccgaag ccggtgaaaa aggtacagga gcagccaaaa
     4621 cgcgatgtca aacccgtaga gtcgcgtccg gcatcaccgt ttgaaaatac ggcacccgcg
     4681 cgcccgacat caagtacggc aacggctgca accagcaagc cggttaccag tgtggcttca
     4741 ggaccacgcg cattaagccg caatcagccg cagtatccgg cacgagcaca ggcattgcgc
     4801 attgaagggc aggttaaagt taaatttgat gtcacgccgg atggtcgcgt ggataacgtg
     4861 caaatcctct cagccaagcc tgcgaacatg tttgagcgtg aggtgaaaaa tgcgatgcgc
     4921 agatggcgtt atgagccggg taagccaggc agtgggattg tggtgaatat cctgtttaaa
     4981 attaacggca ccaccgaaat tcagtaagca gaaagtcaaa agcctccgac cggaggcttt
     5041 tgattattac tcaacaggta aggcgcgagg ttttccttca ggatcaaccg cgacatactt
     5101 aaataatgct tctgtcgctt tatagcgttg cccgatgggt tcagacgcga cttttttcac
     5161 ccacacttca atattaatgc tgaccgatgt cgtccctttc tggacacagc gtgcatagca
     5221 gcacaccaca tcgccgaccg caaccggccg taagaaagtc attccttcaa cccgcacagt
     5281 cacgacgcga ccgtgggcaa tttctttcgc cagaatagcg ccgccaatat ccatttgtga
     5341 cattaaccaa ccaccaaaga tgtcaccatt ggcattggta tcggcgggca tggctaaagt
     5401 acgtaaaaca agatcgccct gagggacgtt atgtgttgta gacatggtaa aaccgactta
     5461 aaaggaaaat cacaggcgcg atgctactat gattttcctg aggagaacag aggagataca
     5521 gcgccagccc cgaaggactg gcagtctggt taggatttat cttcctgcgg catgtggcgg
     5581 tagatataga taccgcttaa caatgtgaag attaaggtaa gggcggtcag gccaaagact
     5641 ttaaagttga cccaaatatt ttgcggcagc cagaatgcga tgtagatgtt tgccagaccg
     5701 caaaggataa agaaaacagc ccaggccaga ttcagcttcg accataccgg ttgcggcagc
     5761 gtgagttctt tacccagcat ccgctgaatt agcggctttt tcatcaccca ttggctgact
     5821 aacagggctc ccgcaaacag agcataaatg actgtaacct tccatttaat aaactcatca
     5881 ttgtggaaga acagcgtcaa gccaccgaag acgaccacca gaacaaaagt gatcagggcc
     5941 atcttctcaa ccttacgaaa gcgaacccag ctatatataa gcacaatcgc cgtggcgacg
     6001 atcagcgccg cagtagccgc atagatgtca taaatcttgt aaaacgcgaa aaagacaacc
     6061 agcggtaaaa aatcaagaaa ctgcttcatt ttacgattcc gtaatcaaat gcttaaggtt
     6121 attggcgaat caacatatac aggcggaaca gatagatgag cagtacggct gaaatcaggt
     6181 tgctcaaggt gttcgccagt acggcaccaa tttccggggt taatgcggca aaagaagagg
     6241 caaaaagcag caacagtgtc tttgccagca accagctcag tactgcgggt gccaccagac
     6301 gcatattcgc ccaggtcagg cgcatgctgc ttcgcatcga ggcaaaaatg cccattttgt
     6361 cctgtaccaa catcaccggt gccagggcca ataaaatggc catgataatt cccggaacga
     6421 ccaccagcat gatgccaatc tgtactaaaa gggtagtcag aaaaatcaga ataaataact
     6481 tgggcaatat cggcgcactg gcaccaatag ctcgcagcgc actgactctc tgacccgcag
     6541 acaccagctg gataattaat attacgcctc cggcgagaat ggcgttaccg attaatcctg
     6601 aaaaagtgga cgccgctgaa gcctgcagca aaatttgttg ctgttccggt gacatattct
     6661 gaaccaggtc gaacaaccca ctactgccgc taacgggcac gccgtcattg agctgcgcaa
     6721 gctgtgcatc actgggtgag aaaacatgcc ctaacaccac tgtgataaac gcacatagca
     6781 acgataccaa cagaatggtc ataaattgat tacggaagaa atttccggtg tcacggtata
     6841 cggactgcgc cgtgatagac atgcactctc cttgagtttt atgcaggtgt taattagcgg
     6901 gcaattgtac cctggttaag cctcgggtgg cagcatcagg tcttgtatgg aaaagcatat
     6961 ctttgtaaag cggaggtaat tgctggcctt caggcgtggg caaaccgcgg caagtggcga
     7021 acgagttaat atttgcgtag cgaaaatatt taaaaattga tttaaatcac attaaccagg
     7081 attctcaatg caacttctaa attaatccag atcaataaat ggtgaattat catatgtaat
     7141 gtgatctatg taggatcatt tgttactcca atgtaggtat attcgtcacg tttttataac
     7201 cataacgacg gagcggatat gaaaaagtta acagtggcgg ctttggcagt aacaactctt
     7261 ctctctggca gtgcctatgc gcatgaagca ggcgaatttt ttatgcgtgc agggtctgca
     7321 accgtacgtc caacagaagg tgctggtggt acgttaggaa gtctgggtgg attcagcgtg
     7381 accaataaca cgcaactggg cctgacgttt acttatatgg cgaccgataa cattggtgtg
     7441 gaattgctgg cagcgacgcc gttccgccat aaaatcggca cccgggcgac cggcgatatt
     7501 gcaaccgttc atcatctgcc gccaacactg atggcgcagt ggtattttgg tgatgccagc
     7561 agcaaattcc gtccttacgt tggtgcaggt attaactaca ccaccttctt tgataatgga
     7621 tttaacgatc atggcaaaga ggcggggctt tccgatctca gtctgaaaga ctcctgggga
     7681 gctgccgggc aggtgggggt tgattatctg attaaccgtg actggttggt taacatgtca
     7741 gtctggtaca tggatatcga taccactgcc aattataaac tgggcggtgc acagcaacac
     7801 gatagcgtac gcctcgatcc gtgggtgttt atgttctcag caggatatcg tttttaattc
     7861 cgcacaaaaa tgaccccgta attacggggt caataaggac atggtataaa gggtattatt
     7921 tcttcgcttc cacgccatca gtttcagagc gaattaagaa tttctcagtt gtttgcggaa
     7981 tatgctgaat tagccagtcg gccatgtgct tttcctcatt taaaatcgct tcgataatcg
     8041 gaattgaggc tgtatcaccg gcattttttg ctgctgctaa gagtgaggta taacaggcga
     8101 tttcaaattg ctcgaagaca tatccgctaa tagagccttt gactatttca tcagaaggga
     8161 atataccacc gattgactgt ccaagcgcag ccattttact catggaatct ttaatgactg
     8221 aacgtgaaat gtcattacga tcaagaatag tttccagttg aacaatctgg tttttggttt
     8281 cactaagatg ttgttcaata cgagcgcgta gttcaggata attatctata cgactggcca
     8341 tggattcaag catagattcg gcttgctttt ccattgcgtg ggcgtcacgt aaccagtcat
     8401 gataatgttc aatacgattc atgaaaatgt cctctttata aaatgactaa aagttaaagt
     8461 tcatatttca ggctttattt tcggctttct tatttacgtt attaagggcc agatcagtca
     8521 gtttgatgtc tgtggccttt tcttcttcca aagtttcttt cagaagcttc gctgctttac
     8581 ggtaacctaa ttgttcagcc agcgtcgcta aggtcccgta actggcgatc tcatagtgct
     8641 cgactttctg tgcagcggca attagtgcgg catcacgtac ttcgtttttc tcggtgcttt
     8701 cgataacctc attagcttct tcaatgagac cttccattgc cacacatttc atgcgcttaa
     8761 ttttcaggtt cgattcagat tccacaactt gatcaattcg ttcaatctgt ccatgagttt
     8821 cctcgaggtg cgcatgaaaa gcctgactta atttttcgtt tgatgttgct ctagcgagtt
     8881 ttgccagtgc acgggttaat tgtttttctg cggtgtaggt atctgaaagc aggtgaataa
     8941 atacatcttc aatggtcttc atattcatat ttttctccag tggaatcact gcgagccata
     9001 cggcccgcag atgaaattat ctttatcagc tgatattaat aattaacggt gaatcaggat
     9061 ttgcctgatt tattaccacc gctttgttga ccgccttttt tacccgcttc agatgcgcgt
     9121 tgcggatcat ttttaaaatt accgccgcta tgctgaccgc ctttacggcc tgcgtcggat
     9181 gccttctcac ggtcttcggc gaaatttcct gaaccaccac gatgttcggc catgttattt
     9241 ctcccgttgc gttgcattgt ttgattaata tgagtgttgt gtgtcgacac tcattaaaat
     9301 tagtcgctaa tgagaattag tcaaattaag cgtaacgaga agaaagtggg aaaatatatt
     9361 atgaggaacg ttctggatat attaacaatt gcctgaggaa taagtgactt agagaaaatt
     9421 atttatttta ctggtgttat gttgcgggat taatttgttt gattatatta ctgttgggcg
     9481 gaaaaataac gtaagttgac gttcgacagg ggtaagcgaa acggtaaaaa gataaatata
     9541 aaatgaattt aggatttttc cggcttcgtt aaagaaagtt aaaatgccgc cagcggtact
     9601 ggcggctgtg ggattaactg cgcgtcgccg ctttcatcgg ttgtacaaaa gctttcagtg
     9661 ccgccagcat tttctctggc tcattaatat gttgctcgat gattttaaca atggccgaac
     9721 cagaaatcgc gcccgcagct cctgcatcaa tcgctgcttt tacctgatcc ggggcggaaa
     9781 taccaaagcc ctgcaatgga ggcgcagcat tgtactcttt cagcttcgtg accagatgat
     9841 tgaggggtaa cgcggcgcgg ttttctgcgc cggtcacgcc cgcacgtgac aataaatagg
     9901 tgtatccacg accgtaggag gctatctggc gcagcaaatc atcgtcggca ttcggcgggc
     9961 aaataaagat aggtgcgaca ttatgacgca acgcggcctg gcggaaggga gcggactctt
    10021 ccactggcac atcggcaacc agcaccgaat cgacgccgac tttctcgcac tgggcataaa
    10081 actcatcaat gcctttgtta aacaccaggt tggcatacat caaaaggccg atgggaatgg
    10141 tcgggtgctt ctggcgaatc agtgccagca tctcaaagca ctgtgccggg gtcacacctg
    10201 ccgcaaaggc acgcagtgtg gcgttttgaa tcgtcgggcc atccgccagt gggtcggaga
    10261 aggggatacc taactccagc gcgtcagcac cggcttcaat tagcgtgtcg ataattttca
    10321 gcgactgctc aatgcccgga tcaccgagcg tgacgaaagg aacgaatgcg ccttctttgc
    10381 gctccttcaa ctgggcaaac agagattcgt agcgttccat cagatttccc ctcgtgcttt
    10441 caaaatatcg tgaacggtga agatgtcttt atcgccgcga ccggaaaggt taaccaccag
    10501 tagctgctct ttttccgggt tttcgcgcat cattttcagc gcatgggcca gggcgtggga
    10561 ggattctagc gccgggatga tcccttcgtg caggcacagc gttttgaagg cttccagtgc
    10621 ttcatcatcg gtaattgaca cgtaatcagc gcgtccagtg ctgttaagat acgcgtgttg
    10681 tgggccgaca gacgggaaat ccagtccggc ggagatggag taagattctt caatctgtcc
    10741 gtcttcggtt tgcatcatcg gcgctttcat accgaaatag atgcccacgc gaccgtgttt
    10801 taacggtgcg ccgtgctcgc cagtttcgat accgtgaccg cctggttcga caccaatcag
    10861 gccgacgtcg gtttcgttga taaaatcagc aaacatgccg atggcattcg aaccgccgcc
    10921 aacacaggcg ataaccgcat ccggcaggcg accttctctt tccagaatct gcgctttcgt
    10981 ttcttcgcca atcattcgct gaaactcacg cacaatggtc gggtaaggat gcgggccagc
    11041 cgcggtgccc agcatatagt gcgcggtttc ataactgccg gaccagtcgc gtagcgcctc
    11101 attacaggca tctttcagcg tcgcggaacc gctatgcacc gggatcactt ccgcacccat
    11161 taagcgcata cgaaaaacgt taggcgactg gcgctcgacg tctttggcac ccatataaat
    11221 gcgacatttc aggccgagca gggcgctggc aagggccgac gccacgccat gctgaccggc
    11281 tccggtttcg gcgatgattt ccgttttacc catccgcttc gccagcaacg cctggccgag
    11341 cacctggttg gttttatgcg cgccgccgtg cagcaaatct tcgcgcttca gatacagcgt
    11401 ggtgttcgtg ccagccgtaa tgttctggca tttggtcagc gcggttggac gcccggcata
    11461 gtttttcagc aggtcgttga actgagcctg aaattcagga tctttttgcg cactgacaaa
    11521 agcttcttcc agctggcgca gagcaggcat caggatttgt ggcacgtaca tgccgccaaa
    11581 ctcaccaaaa taggggttaa gtaatgttgt cattgttcct ttccttaata tgcgcgcagc
    11641 gtctggaaaa ccgaggccaa aagacgtgcg tctttgatgc ccggttgcga ctctacagca
    11701 gaattaaaat caagtccggc gcagccggtt tgtgccgctt ccacgcagtt atctgcgcct
    11761 aagccccccg ccagcagaac gttgccaagc gattgaccgt ttaacagtga ccagtcgaaa
    11821 cgttgcccgc ttccaccctg gccgttgtct aaaacatatt tatcaacgtg ctgaagctcg
    11881 cgggctggca gggtttcacc gacgcttaat gctttccaga tggcaacgtt ttcaggcagc
    11941 gcaccacgca gcgtatcgat atacagctga tcttcattac catgcagttg cactgctgcc
    12001 agcgataaca ccttagcttt gtccaccaca tcggcaatat cgtgattgcg gaacacgcca
    12061 acatactgca acggtgccgc agccatcact tcctgcgcct gttcaacgtt gacgcaacgc
    12121 ggtgacgtcg caacaaaaat caacccaccg taaattgcgc ccgcgtcata agctgcttta
    12181 gcatcttgcc cacgcgtcag gccacatact ttattctcac ccagcaacac ccggcgcacg
    12241 gcggcgtgca aatcgtcatg ggccatcaac gccgaaccaa tcagaaaacc gttagcgaag
    12301 tggcttaact cgcgcacctg agcgtaagta ttgatgccgg attcgctgat taccataacg
    12361 ttgtgcccca gtttcggcgc aagctcgcgg gtacggttga gatcaatcga caaatcacgc
    12421 agatcgcggt tgttgatgcc aacgaccttt gcccccaatg caatggcacg ctccagttcc
    12481 tcttcattac tgacttcggt cagcacaccc atctccagac tgtgggcgac ggcggcaagc
    12541 tggcgatatt gttcgtcatc aagtactgaa agcattaata agcaggcatc ggcctggtaa
    12601 tagcgcgcca gatagatctg gtaagggtcg attatgaagt ctttacataa aatcggctgc
    12661 ggggcgattt gactgacgat ggggaggaaa tcaaagctcc cctgaaaata tttctcatca
    12721 gtcagcaccg aaattgccga agcgtaatgt ttataaatgg cggcaatgcg tgccggatcg
    12781 aaatcatcac ggatcacgcc ttttgacggc gacgcttttt tacactccag aataaacgcc
    12841 gtgcgtgcgc cctgaagtgc atcataaaaa tgtcgcgtgc tcggctgaac cacattctga
    12901 aaactggcca gcggttgctg ttctttgcgg gtttccaccc aaatcgcctt gtctgcgacg
    12961 attttcgcta aaacggtttg catcatttac cctcgtgccg ccagtgcggt gactctgtcg
    13021 taagcggaac cactgcgcag tacctcaaga acggtttgcg cattggcttg cagatcttca
    13081 tggccatgca ggcgcattaa catggcgaca ttcgccgcga cggctgcttc atgggcggcg
    13141 tcgcctttac cttgtaacaa gcgtgttaaa atgtcacggt tttcttccgg tgttccgcct
    13201 gccagttgct cctggtggta gggtgtcagg ccaaaatctt cagcggtcaa ttgatagctc
    13261 ttaatttcgc cgtcatgcag ttcggcaacg attgtcggtg cgtgtaatga aacttcatcc
    13321 atcccaccgc tgtgcactac cgccgcgcgt tgatacccca gcacgcgcaa ggtttcggca
    13381 atcggcagca ccagttccgg gctgtagaca ccaattaacg ccagcggagg atgcgccggg
    13441 ttaatcaatg gtcccagcac gttgaacagg gtgcgggttt ttagttgctg gcgaaccggc
    13501 atcgcgtggc ggaatccggt gtgatatttc ggcgcaaaga ggaaacagac gcctaactca
    13561 tccagcgcct ggcgcgattt atcggcgttc atatcaagat taataccgaa tgccgccagc
    13621 agatccgacg aaccggattt actggagaca ctacggttgc cgtgtttcgc cactttcagc
    13681 ccacaggccg cggcgacaaa cgcactggcg gtagaaatat tgatgctgtt actgccgtca
    13741 ccgccagtac cgacgatatc agcaaacaga taatccgggc gcgggaacgg cgcggcgttt
    13801 tccagtagcg cggttgctgc tccggcgatc tcgttcgggt gctcgccgcg aattttcatg
    13861 ctcaccagcg ccgccgccag ttgttccggt ttcagttcgc cacgtaccac cgctgaaaac
    13921 agctggtggc tttcttgttg gctaagcgtc tgcgcctgat aaagtttttc cagaatcggt
    13981 tgcagcgtgt tagtcggttc cagtttctgc tgcgcccagg ccaatgtttg ttccagcagg
    14041 cgagcgccct gggtagtaag aatggattcc ggatggaact ggaatccaca aacgcgatct
    14101 gcatcgtgac gcaccgccat caccatgcca ttaaaatggg cgttgatggt taaaccggcc
    14161 ggaatgttac tgccaaccag cgagtgataa cgcgccactg gcagcgggtt tgttaatccg
    14221 gcaaacatcg cctgaccgtc atgttcaatg ctggaggctt taccgtgaag aatttcgccc
    14281 gcctgaccga catacccccc ataagcttcg acaatcgcct gatgaccgag gcaaatgcca
    14341 ataattggca gcttgccacg caagcgggtg aggagttccg gcatacaacc ggcttcgctt
    14401 ggcacaccgg ggccaggaga aagcatcagc accggattgc tcattgtcgc caggcgttca
    14461 attaaggtct gcgccggaat atggttgcgg taaatcacca cgttatgacc attgctgcgc
    14521 aactgatctg ccaggttgta agtaaaagag tcgatattat cgagcagcag aatgtcagcc
    14581 atcagaacgt ctcctgtgca tgatgcgcgg tggcaatagc gcgcagtaca gcgcgggctt
    14641 tattacgtgt ttcgtcggct tccgactgcg gaacagaatc aagtaccacg ccagcaccgg
    14701 cttgcacggt ggcgataccg ttttccacca gcgccgagcg gatcactatg caggtgtcga
    14761 gatcgccatg cgcggtgaaa taacctaccg cgccgccgta gctgccgcgg cgacgacctt
    14821 ccgccccggc aattaactgc atggcgcgca ctttcggcgc gccgcttaac gtgcccatat
    14881 tcatacaggc gcggtaagcg tgcagggcgt cgagatcgtg gcgcagctca ccgaccacgc
    14941 gggagacgag atgcatcaca taagaataac ggtcaacttt ggtgagatcg gcgacatagc
    15001 gactgccggg ggtgcaaata cgcgccaggt cattacgggc gagatccacc agcatcagat
    15061 gttcagaaag ctctttatga tcggtacgca tttccagttc gatgcggctg tcgaggtctc
    15121 tgtccagcga accatcggca cgacgaccgc gtggacgtgt tccggcaatg gggtagatct
    15181 caatctggcg gctggtggcg tcatacttga gcgagctctc cggcgacgcg ccaaacaggg
    15241 tgaaatcatt atcctgcata aaaaacatat acgggctggg attactcttt ttcagcacgt
    15301 aataggccgc cagcggtgac gggcagggca gagagaaacg gcgagatggc accacctgga
    15361 aaatttctcc ggcgcgaatc gctttttgca acaaacgcac tacgccaccg aactcttcat
    15421 cgctctggtt acattcacaa tgcatatgcg gcacggaaac caccggcagc ggtggcgcgg
    15481 tttcggtcag ctgctggcga agatcgttca ggcgagcagt gagacgttgt ttttcttctt
    15541 cattcggagc aaaaagactg gcctgaatgc gggtgctttt tttctgatgg tcaatcacca
    15601 tcagcgtttc agcgagataa aaacagaaat cagggcagtt attttccgct gacagttgcg
    15661 gtaaatcttc aaaccccgcc acaaggtcat aagagaacag gccgccgaag aacattgctt
    15721 ctcgttcttc cttcggtaca ttcaacagat tctgcaataa gcggaaagcg tcaaaaaccg
    15781 aaagggagca taagcgggcg tcttcatcca gcagtggact gacagggggg aagcgcagca
    15841 cacggcagtt tggtgattgt tcattttcca cacccgcagg cagggcgtta tccagtagtg
    15901 ccaacagggc ttcgccattg ccggaaagtg cctgaattgt gacagtgtca cctaatgctg
    15961 taatgcgcag cgcactgtct accagcagca ggctttttaa atcatctttg ctgtcgatat
    16021 ctgcggattc cagcagcagc gttgctggac gattcccaca caactggtga aaaagcgcag
    16081 tcgggttgtc gcgataagcg ccttcgcagg ttagcagttc aagagtcggt ttttgtgttt
    16141 gcattgttat tctctaattt tgttcaaaaa aaagcccgct catcaggcgg gctgggtatc
    16201 tgattgcttt acgcatggtg aatacactgc ccgtttcagg aagtgcgcca ccaacctttc
    16261 agtacgaaaa ttgctttcat tgtcgatacc ctttttacgt gaacttgcgt actagttaac
    16321 tagttcaatg attaattgtc aacagctcat ttcagaatat ttgccagaac cgttatgatg
    16381 tcggcgcaaa aaacattatc cagaacggga gtgcgccttg agcgacacga attacgcagt
    16441 gatttacgac ctgcacagcc ataccacagc ttccgatggc tgcctgacgc cagaagcatt
    16501 ggtgcaccgt gcagtcgaga tgcgcgttgg caccctggcg atcaccgacc atgacaccac
    16561 agctgcaatc tcaccagcaa gagaggaaat ttcacgttcg ggactggcgc tgaatcttat
    16621 tcccggcgtg gaaatttcca cggtctggga aaatcatgaa attcatattg tcgggctgaa
    16681 tattgatatt actcatccgc taatgtgtga gttcctcgcg cagcagacag aacggcgcaa
    16741 tcagcgggcg cagctgattg ccgaaagact tgaaaaagcg caaatacctg gggcactgga
    16801 aggcgcgcaa cgactggcgc agggcggtgc ggtgacgcgc ggtcattttg cgcgtttcct
    16861 tgtggagtgc ggcaaagcca gttcaatggc ggatgtcttt aaaaaatatc tggcgcgcgg
    16921 gaaaaccgga tacgttccgc cacagtggtg tacaatagaa caagctattg atgtcattca
    16981 tcattctggc ggtaaggcgg tgctagctca tcctgggcgg tacaatctta gtgctaaatg
    17041 gctgaaaaga ttggttgcgc attttgccga acaccacggc gacgcgatgg aagtcgcgca
    17101 gtgccagcaa tcgcccaatg aacgcaccca gctggcgaca cttgcgcgtc agcatcattt
    17161 atgggcatca caaggatctg attttcatca gccatgcccg tggatcgaac tgggtcgtaa
    17221 actctggctg cccgcaggcg ttgaaggcgt ctggcagcta tgggaacagc cgcagaacac
    17281 cacagagagg gaattatgag ccagtttttt tatattcatc ctgataatcc gcagcaacgt
    17341 ctgatcaacc aggcggtgga gatcgtgcgt aaaggcgggg tgattgttta tccaaccgat
    17401 tccggctatg cgctcggctg taaaattgaa gataaaaacg ccatggagcg tatttgtcga
    17461 attcgccagc tgccggacgg tcacaacttt accctgatgt gtcgcgatct ttctgagctc
    17521 tcgacctatt cattcgtgga taacgttgcg tttcgtttga tgaaaaacaa cacgccgggc
    17581 aactacacct tcatcctgaa agggacgaag gaggtgccac gccgtctgtt gcaggaaaaa
    17641 cgcaaaacca tcggtatgcg tgtgccttct aacccgattg cgcaggcgtt gcttgaagca
    17701 ctgggcgaac cgatgctttc cacttcgcta atgctgccag gcagcgaatt taccgaatcc
    17761 gatccggaag aaattaaaga ccgtctggaa aaacaggtgg acttgattat tcacggcggc
    17821 tatctcgggc agaaaccaac aacggttatt gatcttactg atgatacgcc ggtcgtggtg
    17881 cgtgaaggcg taggtgatgt gaagcctttc ttataacaaa ttgcgttcca cggatggaag
    17941 actatggcag ggatatatcg ctgcattttg ctgttgatag ttgggctttt tttctcttcg
    18001 ttgagttatc cgaaaaacac ggagatccct ttttatgaag aagggatctc gctctttgat
    18061 gttgaagcca ctctgcaacc ggatgggggg ctcgacatca aagaaaatat tcattttcag
    18121 gcgcgaaatc agcagattaa gcacggattt tatcgtgatt taccacgact ctggatgcag
    18181 cctgatgggg acgctgcact gctgaactat catattgttg gcgtcacccg tgatggtgtt
    18241 cctgaaccct ggcatcttga ctggcatatc ggtttaatga gtatcgtcgt gggcgataaa
    18301 caacgtttct tgcctcaagg cgactatcat tatcaaattc attatcaggt taaaaatgct
    18361 ttcctgcgtg agggagattc agatctgtta atctggaacg tgaccggtaa ccactggccg
    18421 tttgaaatct ataagacccg attttcactc aagttccctg atatcgcggg taatccattt
    18481 agcgaaatcg atctctttac tggagaagag ggcgacacat atcgaaatgg ccgcatcctt
    18541 gaggacggaa gaattgaatc ccgcgatccg ttttatcgtg aagatttcac ggtactctac
    18601 cgctggcctc acgctttact tagcaatgcc ccggctccac aaacgacgaa tattttcagc
    18661 catcttcttt taccctccac gtcatcgttg ttaatttggt ttccgtgtct attcctggtt
    18721 tgtggatggt tatatctctg gaagcgcagg ccgcaattta cgccggtaga tgtgattgaa
    18781 accgatgtca ttccgccaga ttacacgccc ggcatgttac gtctcgatgc gaagctggtt
    18841 tacgacgata aaggtttttg tgccgatatc gtaaatctga ttgtgaaagg caaaattcat
    18901 ctggaagatc agtatgataa gaaccagcaa atcctgattc gtgttaatga aggcgcgacc
    18961 agaaataatg cggtattact gcccgcagag cagttattac tggaagcgtt atttcgtaaa
    19021 ggcgataagg tcgttcttac ggggagacgc aacagagtct tacgcagggc atttttacgg
    19081 atgcagcaat tttatctgcc gcgtaaaaag tcttcgtttt accgacctga tgcgtttttg
    19141 caatgggggg gaatggcgat attagcggtc attctctacg gtaacctgag tcccgtaggc
    19201 tgggcaggaa tgagtctggt gggcgatatg tttattatga tctgctggct tcttcctttt
    19261 ttattttgtt cccttgagct tttgtttgcc cgcgatgatg acaagccttg cgttaatcgt
    19321 gtaatcatca ctttgttttt accgctgatt tgttcaggcg tggcctttta ttctctctat
    19381 atcaatgtcg gagatgtatt cttttactgg tatatgccag cgggttattt tagcgctgtt
    19441 gtcctgaccg gttatctcac tggcataggg tatatttttc tgccaaagtt tacccaaact
    19501 gggcagcaac gttatgccca cggtgaagct atcgttaact atcttgcggg taaagaggca
    19561 gcaacacaca gtgggcggcg gcggaaaggg gaaacacgga aactggatta cgcgttgtta
    19621 ggttgggcgg tctcggcaaa ccttggcaga gaatgggcag cacgtatcac cccatcactc
    19681 acagcggctg ttcgcgcccc ggaaattgcc cgtagtggcg ttttattctc attacagacg
    19741 cacctgagtt gcggggccta taccagtttg ttggggcgga gctattccgg tggtggtgct
    19801 ggtggcggag gcggcggtgg ctggtaatca agctgatgtt aatcgaaaca agctttaacc
    19861 ataaagatag tgtagtatgt tgcgcctcaa agccaggcca gttaaaacgt cgagtcgttt
    19921 acttaaggcc tgaagagttc aaacagactt tactatcagt gagtcagatg taagttctga
    19981 ctcactgttg cacaaaagca tatcagacgc ctgggaaggc gacaccaaaa ggaagctcta
    20041 tgagcgaaaa gctacagaaa gtgctggcgc gtgccggcca cggttctcgc cgtgaaatcg
    20101 aatctattat tgaagccggt cgtgtgagtg ttgatggcaa aattgctaaa ctcggcgatc
    20161 gcgttgaaat tacccctgga ttgaaaatcc gtatcgatgg tcacctgatt tcggtacgtg
    20221 agtccgctga acaaatttgt cgcgttctgg cctattacaa accggaaggt gagttgtgta
    20281 cccgtaacga tccggaaggg cgtccaacgg tgtttgaccg tctgccaaag ctgcgtggcg
    20341 cacgctggat tgccgtgggt cgcctggacg ttaatacctg tggtctgctg ctgttcacta
    20401 ccgacggtga actggcaaac cgtttaatgc atccaagccg tgaagttgaa cgtgaatatg
    20461 ccgtgcgtgt atttggtcag gttgacgaca cgaaactgcg tgatttgagt cgtggcgtgc
    20521 agctggaaga tggtccggca gcctttaaaa ccatcaagtt cagcggcggc gaagggatca
    20581 accagtggta caacgtaact ctgaccgaag gccgtaaccg tgaagttcgt cgtctttggg
    20641 aagcggttgg tgtgcaggta agccgcctga tccgtgttcg ttacggtgat atcccactgc
    20701 cgaaaggtct gccacgcggt ggctggacgg agctggatct cgcccagact aactatctgc
    20761 gcgaactggt ggagctaccg ccagaaacca gctctaaagt cgctgtagaa aaagaccgtc
    20821 gtcgcatgaa ggcgaatcag attcgtcgtg cggtgaaacg tcatagccag gtgagcggtg
    20881 gtcgccgttc tggcggacgt aataataacg gttaatttaa gataagtata aaataaaggc
    20941 cctgcggggc cttttcttca tttgaagcat gataaatagt cgcagtattt taatttaatt
    21001 tcatacttgc ttatgaataa tacccggaaa tagccacatt actatttaat tatctgagat
    21061 aaactcaacg tcaagaggtg ggttatgatt agatatagtg gaatatttct atcgctgtta
    21121 atgctttcag gttgtgcaac tgaagcattg aaaaacgaac aagctgaaac tgttattact
    21181 gcgggtaaca aagcagtagc ggcgaatgca agtttcttta aacaatacta tgcttctcgt
    21241 gaaaacttcc ttgttaattt ctacgcgact aatccggatt gtgctctgcg acctgattat
    21301 accattgata taggcggtga tgggaatggc ggaaaattat gccagcgttc agggccaaat
    21361 acaaaacaag tacaattagt ccctttcgag aaagaaaaat tactggcttc aatggaagtc
    21421 attaatagca tgagcgctta tgtggaactt ctgacaaagg atatggcaac tacggaaagt
    21481 cagcaagtgg cattacttaa acaggctctg gatcacgcaa acaacgcaca aacactactt
    21541 ggtgtttcac atagcaaaga tttagctcaa tctgcagcag cggttaaaga tttggtaaat
    21601 tatttcaatg agttatatat aattaaagtc aattctggaa aaataggcaa aacggcagat
    21661 gaaaactggg taagacttat aaagaacatg gcggttttgc gcaatgatat ttatacaaaa
    21721 agcaataaga ttgaaaatta tacctttgcg aatgtgcatg ataatatgtt ttattataat
    21781 acagtatcta aaaactctaa gaatacagag tttaacactc tgaaaaacag gaaggagttt
    21841 ctaagttcag taatcaatgt gaatgaaacc agactacagg catcggcacc tgccatgcct
    21901 gcgattgagg ctttaaatgc ctttatagat aagggtgatt atctaaaaag cttatacgtt
    21961 aataaaacac gcacggcagc tgaacaaaaa agattaaatg aaatattacg cgcacaggtc
    22021 attagtgggt taaaggcgtc tgaggggctg gcaaaattac ttattgccag cggaataatg
    22081 ttataggagg ctttttatga acgatgtgca aaaccgtaga atactggcat tacttgatat
    22141 gctgtataat actttgggta ataaaaccac atgtaatgtt tgtcataaat attttatcca
    22201 tatcctcaca gttatgaaca acctgacgat taactatggt actgaaactt cgccagagtt
    22261 agccaggata aattatgacg atgctaatgc gttaattact gaactgatat tatttattga
    22321 agatgttgac gctgagaatc atgaagcata tccgggacat ttgaatgaga ttacacgtat
    22381 tctgaatttc gctattgccg cttgaatata atcatgatta aagcacctca agaggcttct
    22441 tttgaggtgc tttcacttag tagtcaatcc ctatctgcgc ttttacaccg gcatcaaacg
    22501 catgtttcac ggggcgtaat tcacttaccg tatccgccag ttcaagtata tcccgatgac
    22561 aaccacgacc cgtaataatg acggtttgct gatgtggacg ttcatttaac gccttcacca
    22621 cttcttcaag tggcaaataa tcatacgcca ccatataagt cagctcatcg agcaaaacca
    22681 tatccagtga aggatcggcc agcattcgtt tggcatgttg ccagacttca cggcaggccg
    22741 cggtatcaga ttcgcgattt tgtgtatccc aggtaaagcc cgttgccatc acctgaaact
    22801 caacgccatg tggctccagt agattgcgct cgccattagg ccaggtgcct ttaataaact
    22861 gcacgatacc cacttttttg ccatggccta ctgcacgagt tgccgtacca aatgccgcgg
    22921 tggttttgcc ttttccattg ccggtaaaaa cgatgataat accgcgttca tcctgggcct
    22981 gggccacacg agcatcgact ttttctttca ctcgctgctg acgctgttgg tagcgttcat
    23041 cactcattgg gaaattcctg gtttacggcc cggttgggcg tcaaaggtca tgccggtttt
    23101 acggcggctg tcatcgccca tcagccagag gtagagcggc atgatatcag cgggtgtttt
    23161 aagtttctgt ggatcttcgg tcgggaaggc gctggcccgc attgcggtgc gcgtaccgcc
    23221 tgggttaatg cagttgacgc gcaggcgctg ctgatattca tcggccagta cctgcatcat
    23281 cccttcggtg gcaaatttcg acgctgcata tgcgccccag ttggctcgtc cctgacgtcc
    23341 aacgcttgat gaagtaaaga ccagtgaacc ggcgtccgat ttgagtaata aaggaagcag
    23401 tgcctgggtg agcataaagg tggcattaac gttgacctgc atgacgtcct gccagacctg
    23461 cggattttgt tcgctcattg ggcaaacatc gccgagcaat ccggcattat gcaaaacacc
    23521 atccagacgc ggataattaa cggcaatgcg ctgtgccagt tgttggcaat cttcggacgt
    23581 gcaggtcagc aaatcgagaa taaaccactg tggctgacgt ccagtttctt cgtttatgtg
    23641 gctggctacc tgacgtaatt tttcttcatt acggcccaac agaatcactg tcgcaccata
    23701 gcgtgcatac gtcatcgcgg cttcacggcc aataccatcg ctggctcccg tcaccaggat
    23761 aatgcgatca ttgagtaaat cttgttttgg ctggtaatgc atggctactc ctcaacgacg
    23821 ttgtctgtcg cgctgccagt gtactttatg actttggggc tttatgcctg aaacagaagg
    23881 gcatttcaat cagcctgggg cgataacacc gcaaaatgga tactttgtca tactttcgct
    23941 gcaataacat ctctgcgaga cggcttaaca tgcctgttgt aaactgtgag ccaaagcgtt
    24001 gtttaaccaa ggtgggaact cgtggaattg ttgtctgaat atggtttgtt tttggcgaaa
    24061 atcgttaccg ttgtgctagc gattgcggcg attgccgcca ttattgtcaa tgttgctcaa
    24121 cgtaataaac gccagcgtgg cgagttacgg gtcaacaata tcagcgaaca gtataaggag
    24181 atgaaagaag aactggctgc ggcgctgatg gacactcatc agcaaaaaca gtggcacaaa
    24241 acgcagaaga aaaagcacaa acaagaagcg aaagcagcaa aagcgaaagc caaactgggc
    24301 gaggtggcaa ctgacagtaa acctcgcgtc tgggtgctgg attttaaagg cagcatggac
    24361 gcccatgaag tgaactcgct acgtgaagag ataacggcgg tactcgcagc attcaaaccg
    24421 caggatcagg ttgtgctacg tctggaaagc cctggtggca tggtgcatgg ttacgggctg
    24481 gcggcttcgc agctgcagcg tctgcgtgat aaaaacattc ctttaactgt tacggtagac
    24541 aaagttgctg ccagtggcgg ttacatgatg gcctgtgtgg cagacaaaat tgtttccgca
    24601 ccgtttgcta ttgtgggttc tattggcgtg gtggcgcaaa tgcccaactt taaccgcttc
    24661 ctgaaaagca aagatattga tatcgaactg cacaccgccg ggcagtataa gcgcacgctg
    24721 acgttgctgg gtgaaaatac cgaagaaggg cgggagaaat tccgcgaaga gctgaacgaa
    24781 acgcatcagt tatttaaaga ttttgtgaag cgtatgcgtc cgtctctgga tattgaacag
    24841 gtggcaacgg gtgaacactg gtacggacaa caggcggtag agaaaggcct ggttgatgaa
    24901 atcaacacca gtgatgaagt tattcttagc ctgatggaag gccgtgaagt ggtcaatgta
    24961 cgctatatgc agcgtaaacg actcattgac cgattcaccg gcagcgcggc agagagcgcc
    25021 gatcgattgt tgctacgctg gtggcagcgg ggtcaaaagc cattgatgta aaagacaaac
    25081 gcgaggctaa gacctcgcgt tttgcttgtg cacagaaaac ccccagctag gctgggggtt
    25141 ccggaaagct ttcagcttt
//
LOCUS       DAPOZO010000070        25051 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPOZO010000070 DAPOZO010000000
VERSION     DAPOZO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.070"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(32..562)
                     /gene="cybB"
                     /locus_tag="ROI21_003142"
     CDS             complement(32..562)
                     /gene="cybB"
                     /locus_tag="ROI21_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDX5112034.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHEMLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            751..1752
                     /gene="gap"
                     /locus_tag="ROI21_003143"
     CDS             751..1752
                     /gene="gap"
                     /locus_tag="ROI21_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDX5112035.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDIVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKNATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(1794..3234)
                     /gene="aldA"
                     /locus_tag="ROI21_003144"
                     /pseudo
     CDS             complement(1794..3234)
                     /gene="aldA"
                     /locus_tag="ROI21_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(3431..4231)
                     /locus_tag="ROI21_003145"
     CDS             complement(3431..4231)
                     /locus_tag="ROI21_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDX5112036.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(4347..4724)
                     /locus_tag="ROI21_003146"
     CDS             complement(4347..4724)
                     /locus_tag="ROI21_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112037.1"
                     /translation="MDECFINITPTSLSFKNAELSDLSYKNARLELTLICPAIKSEVK
                     LSFDWIHSFRVTDEGDLLGMSGIHGINQTGIYRVVNSAYFAWFTMQSCEIYKNNVIEH
                     YVIATSNDVIDVLSTVSPQIQYD"
     gene            complement(4844..5293)
                     /locus_tag="ROI21_003147"
     CDS             complement(4844..5293)
                     /locus_tag="ROI21_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112038.1"
                     /translation="MNLSNTKCDKLSFYEEKIRWRMRRQISNAEAIFYENQEEHIQRF
                     LGRYTCRKPVLVYWESEQRWTSVNGLEVMSMFDEYCHHIYLDDINKKIQAELPEDKTL
                     GFKRQLQFFILGDEKIRIWVPQGELYFALMNVLQMFPLKKMVDDWTR"
     gene            complement(5280..5618)
                     /locus_tag="ROI21_003148"
     CDS             complement(5280..5618)
                     /locus_tag="ROI21_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112039.1"
                     /translation="MKNLDNIIWHDGVLVETRMECHHTEYNFILTAELYVDEFTAGRE
                     RKRFEFTGVEHLSQVMDIAELIDNQNAGNIKDAVIVYKAKKYEISLHLFGGFLNFIFK
                     TMRVVDNEFE"
     gene            complement(5903..9805)
                     /gene="hrpA"
                     /locus_tag="ROI21_003149"
     CDS             complement(5903..9805)
                     /gene="hrpA"
                     /locus_tag="ROI21_003149"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDX5112040.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEDSGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQGLKDALKDKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            10006..10611
                     /gene="azoR"
                     /locus_tag="ROI21_003150"
     CDS             10006..10611
                     /gene="azoR"
                     /locus_tag="ROI21_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDX5112041.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKSHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            complement(10665..11981)
                     /locus_tag="ROI21_003151"
     CDS             complement(10665..11981)
                     /locus_tag="ROI21_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDX5112042.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGFLNQFTATQDLNNHDI
                     PSQVFGWETAIPFLPWTIFPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCLRLEPVSKITAGVYLGAFPQHIPAQNVVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSRIVLKEEHKAMLKLWENR"
     gene            complement(11971..13728)
                     /locus_tag="ROI21_003152"
     CDS             complement(11971..13728)
                     /locus_tag="ROI21_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDX5112043.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEEKRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRFIDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVTHLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSEILLRDYSEVNVAQGQE
                     MIAQRGMSGRARFEQGDAFNPAELSTLTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(13744..14640)
                     /locus_tag="ROI21_003153"
                     /pseudo
     CDS             complement(13744..14640)
                     /locus_tag="ROI21_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(14640..15245)
                     /locus_tag="ROI21_003154"
     CDS             complement(14640..15245)
                     /locus_tag="ROI21_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDX5112044.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLNKHHVTANHITLAALALSLLSGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            complement(15416..17722)
                     /locus_tag="ROI21_003155"
     CDS             complement(15416..17722)
                     /locus_tag="ROI21_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HDX5112045.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVVSSTNSEFMVRAGDKNDTENVKKALTIINPDAYYWGLINDEDALKEIF
                     KRSNIRIAGNVCNQMKKEALFRPKPSPELVQELQMLDEGKVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLSYNQAMQLANDSSNDVVTSLASKLAEMKHHGQLLRM
                     TPQESHKIAVYLYQKFENDDDLIGTLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSGLISHGEVDDQMLLNATSLIRSEGWDFLESALVSWDNLPAVVLKELQQNIPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDKLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNHHTPPAVLAAEIDPEWWIVAMNNPRFPVDILKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(17786..18646)
                     /gene="pdxI"
                     /locus_tag="ROI21_003156"
     CDS             complement(17786..18646)
                     /gene="pdxI"
                     /locus_tag="ROI21_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDX5112046.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNIWLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(18854..24934)
                     /locus_tag="ROI21_003157"
     CDS             complement(18854..24934)
                     /locus_tag="ROI21_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDX5112047.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIAETSDESRKSSKISCPAN
                     IKSLSKEQLEKLPAECKSVEDNELLPWAAVGLAAAVTGVAVYALHDNNNHHDDPSPVP
                     DDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPV
                     PPDDGGDTPVPPDDGGDTPDDGGDDTPDDGGDDTPVKHDPVVYKNDVTWDQDAKTLKL
                     RDTTFTYAKNDDGSYTLTGPGGTTTIVKDWLVDEAANTASFNGVNPGGTSTWRYDEAG
                     KIEIIIESLTTADGTTGEHFHINDATITDHGGNIALNGGVVINVDGNDITLNNDGKTT
                     AIGEGSVVGILTGDDITITNNGETEVDGGTAVIVNGDRTTLNVAGDSTFTNGGTGSLI
                     NGDDAKVDNKGTMNVDGENSTGSKINGNDALVKQEGDLFVSGGAKGIDVTGNSTIISN
                     KGNITVVDEKSIGILLDGNDSAFINMGDISVSDGSASENAIGVLIEGDGSTLINVGDV
                     SAQNAATAIKIVGDNSSIALAGLVETGGFSTGLEITGDNNTLTMSTYALNVIGEQSAG
                     VIVSGDNNNLEITGSMVVDGDSVGTLVTGNGGKVSQKGDLFVSDAGRGIDISGDRVTL
                     GNTGKITVEDANSVGIIIEGDDAVFKSTGEINVSLDGQGAAISGDREDVSLSGDINVT
                     QQRDSNGVFVGGTGVDMSGDDSHISLDGNINLVSLMGDSPTISGDSLTGLSVGGDNNT
                     VDLSGNININVGSGYLNDQAVLVGVDVTGENNTINLEGGINITGDSGGHSIKGVSVTG
                     NNVMNISGHSQMNSRQVRGAFTLVSVADGGHAIFDENSVTDFDVRARDTNWYLDDALI
                     TATGIGSEIENHGIINTTDAQDLMMARAGAEIKNTGTINVKPGTEAYARAGVMRAIDE
                     GSRAVNGAGGIINLVSTTVPTGSSGSYEFPVVWYHGTNFALLASNYGDVTNESGATIN
                     IHGPGVYGVAASRGTATNAGVINVDGFIPSVDENGDITDEAYWDFNAYFLMAGGALAG
                     STDAGHGDADIVNTGIINVKNQGFGMLAMEGGTVINQGEINLLSDEGLTQQHEGQLFA
                     MGAIRSGTAINDVTGVINIDTDIGQAFYGDGTGTIVNYGTICSFGICQSPEQYNPTDS
                     YVSVQYTGGDILVAEGESTNFINPVLITDATPGSTINAGTVSGSNINVQSGTLENTST
                     GIINTLVKLAPDAVIDNAGSMGNIDAQGGTLNNEGTINGNVTMYAGASTNLVNNSGTI
                     SNVVQKSGVFNNSGTVSKRIIQTSGIFNNLQDGKVDNGSALTGDAWVNNEGTWRLGSS
                     SGGNNAGMLEVNNNAVFNNSGEFILDNNKNAVHINQSGTLYNTGHMDISNSSHNGAVN
                     MWGGNGRFINDGTIDVSAKSLVVSASNAGDQNAFFWNQDNGVINFDHDSASAVKLTHS
                     NFIAQNDGTMNISGTGAVAMEGDKNAQLVNNGTINLGTEGTTDTGMIGMQLDANATAD
                     AVIENNGTINIFANDSFAFSVLGTEGHVVNNGTVVIADGVTGSGLIKQGDSINVEGVN
                     GNNGNNTEVHYGDYTLPDVPSPNTVSVTPGSDATDGGMNNLNGYVVGTSADGSAGKLK
                     VNNASMNGVGINTGFTAGTADTTVSFDNVVEGSNLTDADAITSTTVVWNAKGSTDANG
                     NVDVTMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAELNSALKQVSGS
                     QATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNNTEYDMLALRKT
                     IDLSESQTMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSFDNGMNWNNALR
                     YDVHNLDSSRSIAFSNTNKTADTDVKQQYLEFRSEGAKTFEPSEGLKVTPYAGVKLRH
                     TLEGGYQERNAGDFNLNMNSGSETAVDSIVGLKLDYAGKDGWSASATLEGGPNLSYAK
                     SQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDAYHWKEDGISDK
                     GVMLNFKKTF"
BASE COUNT         6514 a         6572 c         5508 g         6457 t
ORIGIN      
        1 ttttagtaca ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc atcatgcgta
       61 gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc agccgcagcg tgcaacccga
      121 tgacaaaata tcccagattc gccagcattt catgccacga ctttaagcta tcaacccgtt
      181 cgaaattggc ctctgatgca taaggcatcg tcaaaccaaa cgcaaaccac gggttgcctc
      241 ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga taaatcacca
      301 aatgtcccaa atgcgccagt cccgtcatca tcggttttgg cttaggtata atcggcgggg
      361 ttgggtattt cagccttaac aaaagacgaa cgaccatcag cacgagaatt gatatgccac
      421 aggaaacatg aatcatgttg atgagtggcc gatcgctacg tgggaagaaa ccccgaaact
      481 ccattgcgca atacgctgca ataaccagca aaaagaccag ccagtgaatg ctgatttgta
      541 accttgagta tttatttccc ataacatttc ctgctttaac ataatttgcc gttaacataa
      601 cgagcttttc ttaaaatttc attaaatatt gttcacccgt tttcagtacg ttacgtttca
      661 cggcaagaat gtagattgcc catcccgcca tcgtggtcta agcctggaaa ggatcaattt
      721 tcatccgaac gttcctgaca ggagaagacc atgagtaaag ttggtattaa tggttttggt
      781 cgtatcggtc gactggtgtt gcgtcgatta cttgaagtca aaagcaacat agacattgtt
      841 gctattaatg atctcacttc cccaaaaatt ctcgcctacc tgttgaaaca tgattcaaac
      901 tacggaccgt tcccctggag cgttgatttt acggaagatt cacttatcgt tgatggaaaa
      961 agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc gaaaggcgca
     1021 gaaatcattg tcgaatgtac tggcttttat acctccgccg agaaatcgca ggcgcatctt
     1081 gatgctggtg cgaagaaggt gttgatttcc gcccctgccg gtgaaatgaa aaccatcgtt
     1141 tataacgtca atgacgacac actggatggc aacgacacca ttgtttccgt ggcgtcatgc
     1201 accactaatt gtcttgcacc gatggccaaa gccttgcacg acagtttcgg aatagaagtc
     1261 ggcacgatga cgaccattca tgcctatacc ggcactcagt cactggtgga tggcccgcgt
     1321 ggtaaagatc tacgcgcttc acgtgcagcg gcagaaaata tcattcccca cactacgggg
     1381 gcggcaaaag ccattggtct ggtgatccca gaactgagcg gcaaactgaa aggtcatgca
     1441 caacgcgtac ccgtgaaaac cggttcggtg actgaactgg tgtctattct tggaaaaaaa
     1501 gtgactgccg aagaggtgaa caacgcgctt aaaaacgcga caaacaataa cgagtcattt
     1561 ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt cggttcggtg
     1621 tttgatgcca cgcaaaccga aattaccgcc gtgggcgatt tacaactggt gaaaacggtc
     1681 gcctggtacg ataacgaata tggcttcgtc acgcagctta ttcgcaccct cgaaaaattc
     1741 gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctctttcac tcattaagac
     1801 tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc atctgcgccg
     1861 ccaataccgg atttacgcca tccggcgtgg aagccttgca tagcttcgaa gttttcacgg
     1921 ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac gttcagattt
     1981 tgggtataga ttgatgaggt caggccgtaa tcactgtcat tcgccattgc gatagcatct
     2041 tccagcgtgt caaatgcgac caccggcaga accgggccaa aggtttcctc atgcataatc
     2101 gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc tttcccctct
     2161 accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc cactttttgc
     2221 tcgacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc gttgcgttct
     2281 gcggggttac caaattgaac cgcctgcaac gcttcaccca gccgattgac gaactgatca
     2341 taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc actattaatg
     2401 acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc cattacgata
     2461 gctggtgctt taccccccca attccaggca cactttggtg atgtttttcg ccgcagtcgc
     2521 catgatcttt tcacctgcag agacgctgcc tgtcatactg accattgcga cctttgggtt
     2581 acccgccagt tcttgcccaa cggtttcacc acgccccaga acaaggttaa acacgccgcg
     2641 cggaaggcct atttcatcga cgattttggc gaatgcaatc gcattgtttg gcgtaaattc
     2701 actgggttta atgacaatgg tattaccagt caaaagagcg ggagccattt tgcgggcaat
     2761 gaggaagaac gggaagttcc acggcagaat gccggtagtc acaccaagcg cacgtttaaa
     2821 cagaagaata ttttctcctg gacgatcgct ttgaataatc tcgccctcgt aacgccgtgc
     2881 ccactccgcc atgtaatcga tatagtccgc agtaaaagcc acttcgactt cagccagctg
     2941 ctggatcttg cccccttctt caacaatcag cgcactgatt tcactggcgc gttcgcggat
     3001 cccgacggag attttgcgca accaactggc gcgttcaata gcaggcaacg cttcccattc
     3061 tggttgtgca cgttctgctg catcgattgc cttacgggca tcctcggcct gaccatcggg
     3121 aatgcgggaa atgacagcct ctgtagcagg gtttaccaca tcaatccatg cgtctccacg
     3181 ccaggtaaca aactgtccat caatatacat aggatgttga acgggtactg acatgagcgg
     3241 ctcctgtgat ttatatgttt gtttttggta aatacattgt taattaacat caatcatcag
     3301 agagacggca tcatgaaagg acaattctgt gaagggcttc ataaaaacaa gtgtaaaagg
     3361 aataagaggc agaaataaaa ggcaggagtt atgcccgttt gcaaacaagt ggttcacaaa
     3421 cgggcaattt ttaacgtaac gagcgacttt ccatcgcctc gatgagcacc gcatcgtgtt
     3481 tcagcgtttg ccatgcatgg atgacttctg ccggaaaatc aacgtgaacg ataaaatctc
     3541 gcccgcgggg accgtagcca tcgctatcat cgcgtaaacg cggcagctcg ccagtgagta
     3601 gtgagagata acgctcaacc ggccataatc cttgcaacgg attaacaaag attacactgt
     3661 ctgcgttgtt tcctagctga ggaacgaatc cgggataact taaccagcga ggtgcatctg
     3721 gattgtcccc ggtcatacgg cggaacgtcg ccatcgtgcg ccgctgcatg gtggggtcct
     3781 gaacaacgat agccgtatga actcgttcta cggcctgatt caatagcgcg atgctaaagc
     3841 gtgcgttttc accgcagttt gttgactggt cttcaatcca gattttttca tgcggaatat
     3901 gccagaactg atgagcgata tccgccagga tggtcgcttc tgctctgcca gtggtgcgaa
     3961 tagtgttgta gtgcggatgc tgtgcgatgg cgctatacaa aaaagttgtc gagtgaccga
     4021 taccaccact gatcagtaaa ggaatttgct gatcgcgagc aatcttacat gccgcgtcga
     4081 tagtcggcat aaccgcattg cctgcaagga tcacgcaatc ggcctgatac ggcacctcac
     4141 cggagaaatc atcctgcgcc agccactgtc cgataacatt tatggcatct atggttgccg
     4201 gcgaaagcgt cggaaacggg gtaatgttca tggttcctcc ttatcgttcc cttaagaata
     4261 ctgcgtgaga taataatgag aagcggagca ctctgaaagg aagatgatgc tttttctacc
     4321 tcgccatcgc gaaccagaca ctggttttag tcatactgta tttgcggaga aactgttgat
     4381 aaaacatcaa taacatcatt tgacgtggca atgacataat gctcgatcac attattttta
     4441 taaatttcac aactttgcat agtaaaccat gcaaagtagg cggaattcac tactctatag
     4501 atccccgttt ggttaatacc gtgaattcct gacattccta acaaatcacc ttcgtcagta
     4561 acacgaaatg aatgtatcca gtcaaatgat aacttaactt ctgacttgat cgccggacat
     4621 atgagggtaa gttcaagcct ggcattttta tagcttaaat ctgatagttc ggcattttta
     4681 aaagacaatg aagtcggagt gatatttata aaacattcat ccatgttaat accccttcat
     4741 atatgagact gctctcgata gacgacacat taaccttctg caaaaacggt gcgagtctgt
     4801 ttacgtacaa tgtataatcg atggacatta ctgtgatttg aaattacctt gtccagtcat
     4861 caaccatttt tttcaggggg aacatctgca atacattcat taatgcgaaa tataattccc
     4921 cctgtggcac ccatattcta atcttctcat cccctaaaat gaaaaattgc aattgacgtt
     4981 tgaaacctaa tgttttatct tcaggtaatt cagcttgtat ttttttattg atatcatcaa
     5041 ggtagatgtg atgacaatac tcatcaaaca tactcattac ttccagaccg tttacagatg
     5101 tccatctttg ttcactttcc caataaacaa gaaccggttt gcggcaggta tatctgccta
     5161 aaaagcgctg aatgtgttct tcctgattct cgtaaaatat tgcctctgca ttcgagattt
     5221 gccgacgcat tcgccagcgg attttttctt cataaaaaga caatttgtcg catttggtat
     5281 tactcaaatt cattgtcaac aactcgcatg gttttaaata taaaatttaa aaaaccgcca
     5341 aacaaatgaa gtgaaatttc atacttcttt gccttataaa caattaccgc atctttaata
     5401 ttacctgcat tttggttatc tataagttca gcaatatcca taacctgcga taaatgttcc
     5461 actcccgtaa actcgaacct ttttcgttcc cttccggctg taaattcatc aacataaagt
     5521 tctgccgtca atataaaatt atactcggtg tggtgacatt ccattctggt ttctactaaa
     5581 acaccatcat gccagataat attgtctaaa tttttcatac tattaatcca atccattact
     5641 ttctgataaa atttcctctg gaagtttttc atagaaagag attaaagcct gaccgacgca
     5701 atcaagctct attattcgct cataatacga tctcattaaa atatcatgct tactcattgc
     5761 aatacgtcct gtttaccatg ggcagagtat gaatgtaact tatgtaaaat agtaccgccc
     5821 ttgctatttt tcagaattca taaagaaaaa acccggcgct aaatgcttac cgggttttag
     5881 atttatcagg caaatagcag tgttaaccgc taatctgctc catcgcctgc aaaatacgct
     5941 tatctgaaat cggataaggc gtaccaagtt gttgagcgaa gtaactaacg cgcaactctt
     6001 ctatcatcca acggatctct ttcacgtctt catcctcacg acgtgcgggc ggcagtttgt
     6061 tgatccattg ctgccacgcc tgctggacgt tttcgacttt cagcatctga gcacggtcac
     6121 gatgcggatc aaccgccagt ttttccagtc gtttttcaat cgcctgcaaa taacgcagcg
     6181 tgtcgcccag ccgtttaaaa ccgttgccag tgacaaaacc gcgatacacc aacccgccca
     6241 tctgcgcttt gatgtcagaa agccccagcg ccatagtcat atccacccgc cctttcaggc
     6301 gtttgttgat attgaagact gcagtaagga tttgctcgac ctgcttcgca atatccacca
     6361 ccgtgtcgtt cagttcagcg cggacttttt catgcagcgc agcaaagcct tcttccgtcc
     6421 agaccgggcc accattggcg tcgatcagtt gatccacacc gcaggagata cagtcgtcga
     6481 tcagctccag cactttgcca tacgggttaa agtacagccc cagcttggct ttgtttggca
     6541 acttctcatg caaatatttg attggcgagg gaatattcag cagcagtaga cggcgaagac
     6601 cgttccacat tgcctgcttt tgttccagcg gattatcaaa cagtttgatc gccacgctgt
     6661 cgcgttcatc cactagcgct ggccacgcct tcactttata gttgccacgc ttctgttcgt
     6721 agctttccgg cagctgacca aaactccaga tatgtaagcc gctctgctcg ataccgtcat
     6781 ccgccaccgc agacagcgtt tcctgcactt tgtctttcag cgcatctttc agaccttgta
     6841 gagagcgccc ttcttttagc ttcttgtttt tgtcatccac cacgcggaag gtgattttca
     6901 ggtgatcagg cacctgatcc cagtgccagt cttcgcggtc aacggtaacg ccggtcatcc
     6961 gccgtaactc gcgctcaagg ctgtcgagca acggtaactc cagcggtgtg acgcggccta
     7021 aaaacgcttc ggcgtagttt ggtgcaggca caaaattacg gcgtaccggt ttcggcaacg
     7081 atttaatcag tgcgatgatc agttcacgac gcaggccggg gatttgccat tcaaacccgc
     7141 tatcttctac ctgattaagc agcggcagcg gaatatgcac ggtcacaccg tccgcgtccg
     7201 cgccgggctc aaactgatag ctcaaacgca gcttgagatt gccctgatgc cagaagttcg
     7261 ggtaatccag cttgctgatt ttttccgccc cttctttgat caacatgctc ttttcaaagt
     7321 tgagcaaatc aggcgtttcg cggctgactt ttttccacca gctatcgaag tgacgagcgg
     7381 agattacatc gtggctgatg cgctggtcgt agaactcaaa cagcgtttcg tcatcaacca
     7441 gaatatcgcg gcgacgtgat ttgtgttcca gctcttccac ttcggcccgc agtttcaggt
     7501 tttcacggaa gaatgcgtgg cgcgtctgcc agtcaccttc caccagcgcg tggcgaataa
     7561 agagttcacg acataacgcc ggatcgatct ggctgtagtt gaccttacgc gcagcaacaa
     7621 tcggcaaacc ataaacagtg actttttccg ttgccatcac cgcgccctgc gcccgttccc
     7681 agtgcggttc gctgtaggtg cgtttaatca aatgctgagc aacgggttct acccattcag
     7741 ggtcgatacg cgcagcaatg cgcccccaca ggcggctggt ttctaccagt tccgccacca
     7801 ttacccattt cggcggcttt ttaaataagc cggaaccggg gaaaatggag aaacgcgcgt
     7861 tacgtgcgcc ggtatattct tgtttatcgg catctttcat gccgatatgg gaaagcaaac
     7921 cggtcagtaa ggcgatgtga atttcgcgat actccgccgg ttcgctgtta accggaatgc
     7981 caagttcttt caccacctga cgcaactggg tgtagatatc ctgccattcg cgcacgcgca
     8041 gatagttgag ataatcggta cgacacaggc gacggaaggc gttggaagaa agcgcctttt
     8101 gctgctcgcc aagataattc cacagattta caaacgcgag gaagtcggat tctttgtcgt
     8161 ggaagcgacg atgtttttcg tccgatgcct gctgtttgtc cattggtcgc tcacgcggat
     8221 cctgaatgga gagcgcggac gtgataatca tcgcctcacg cacgcagcca tgtttctgcg
     8281 cttccagcac catacgcgcc agacgaggat cgacaggcaa ttgcgaaagc tggcgaccga
     8341 gcggcgtcag tttataggcg ctggcctgtt catccgtggt gatcgcgccc agttcttcga
     8401 gcagacgcac gccatcctgg atattgcgtt tatccggtgc ttctacaaac gggaatgcgg
     8461 cgatatcgcc cagccccagc gcagtcatct gtaaaataac cgaggccagg ttggtacgca
     8521 gaatctccgg atcggtaaac tccgggcgcg aaaggaaatc gtcttcggaa tagagacgaa
     8581 tacagatccc ttcggacaca cgaccacagc ggcctttacg ctgattggca gacgcctgcg
     8641 aaatgggctc aatcggcaaa cgctgcactt tagtgcgata gctgtagcgg ctgatacgcg
     8701 ccgtacctgg gtcgataacg tatttaatcc ccggtacggt cagcgacgtt tccgcgacgt
     8761 tggtcgccag cacaatgcgc cgtccactgt gcgactggaa cacgcggttc tgttcgctgt
     8821 tcgaaagccg cgcataaagc ggcaagattt cggtatggcg taagttcagc ttgttcagcg
     8881 catcggcggt atcgcgaatt tcccgctcac cactcataaa gatcagaatg tcgccagggc
     8941 tttcctgact cagttcgtct acggcgtcaa aaatcgcctg caactgatcg cgctcggtgt
     9001 catcggcttc ttcaacaatc gggcgatagc gcacttccac cggataggtc cgaccggaga
     9061 cttcgataat cggcgcatta ttaaagtggc gcgaaaagcg ttccgggtcg atagtcgcgg
     9121 aagtgataat gatttttagg tcaggacgcc gcggcagcaa ctctttcaaa tagccgagca
     9181 gaaaatcgat attcaggcta cgttcgtgcg cttcgtcaat aatgatagtg tcgtactgca
     9241 tcagcaggcg gtcttgctgg atctccgcca gcaggatccc gtcggtcatc agcttgacca
     9301 tcgtgttatc acttacgtga tcgctgaaac gcactttgta accgatgcaa ccgcccggct
     9361 ccgttttcag ctcttccgca atacggttcg ccaccgttcg tgccgccagg cgacgcggtt
     9421 gggtatgacc gatcagccct ttaataccgc gccccagctc catacagatt ttcggtagct
     9481 gagtcgtttt accggaaccg gtttccccgg cgacgatcac cacctggtga tcacgaatcg
     9541 cttcgagaat gtcctgcttt ttctgactaa ccggcaaatt gtccggataa gtgatttccg
     9601 gtcgtgccgc ttcacgcagc aggactttcc ctgccgcatg gtcaatctct ttcgccatct
     9661 cctggaaaat ggcctgttgt gcatcaggat ttttaacctt cttcacgccg tgcagacggc
     9721 gagaaaaacg cagtctgtcg cgcagcatca gcgaatccag ccgctgctgc aaggccgtaa
     9781 aggtcaattt ttgttgttct gtcataacgt tagtgggcag taggctgcct gattaatttt
     9841 cttttaaatg atagatatag agtaccacat cgcggctttc tatgcgttgt tcaataaatt
     9901 cgaacatagg cttcgattta ttgcgctatc tctgtggcag aattgtgaat aaagtgtcaa
     9961 caagcaacgg ggcatcgccc attcaaacat ctataaggaa acaccatgag caaggtatta
    10021 gttcttaaat ccagcatcct ggcagggtac tctcagtcta atcagttgtc cgattatttt
    10081 gttgaacaat ggcgcgaaaa gcactccgct gatgaaatca ccgtgcgtga cctggctgca
    10141 aatccgattc cggtactgga tggcgaactg gttggtgcac tgcgtccgag cgatgcgccg
    10201 ctgactccgc gtcagcagga agctctggcg ctttccgatg agctgattgc cgagctgaaa
    10261 tcccacgacg ttatcgttat tgcggcaccg atgtataact tcaacatctc gactcagttg
    10321 aaaaactatt ttgacctggt tgcacgtgca ggcgttactt tccgctatac cgagaacggt
    10381 ccggaaggtc tggtaacggg taaaaaagcc atcgttatta ccagccgcgg tgggatccac
    10441 aaagatggac caacggacct ggtgacgccg tatctgtcca cgttcctggg ctttatcggc
    10501 attactgatg tgaaatttgt cttcgccgaa gggatcgcat acggtcctga aatggcagcg
    10561 aaagcgcagt ctgacgcgaa agcagctatc gacagtattg ttgctgaata accggacacg
    10621 atcttgatgt ggactgtaga tattcagtcc acatctcaat ccgcttacct gttttcccat
    10681 aatttcagca tcgctttgtg ctcttcctta agcacaatcc gcgagcgtct ggctcgaata
    10741 taactaatcg cttcatcaac ggttttacaa tgtccgtaac ataacagcca tgccgccacc
    10801 accagcgcac tgcgcgataa tcccaacgcg caatggacca gaacgctgcc ttgctcttcg
    10861 cgtaatgttt ccaacatcgc cacggcctgt cggagctccc cctcttccgg aaccaccaga
    10921 tccaacatcg gtacacaaaa atagagtcga tcttttgtcg ctcgtccgcg agggaattca
    10981 aaggtgacgt ccagaaccac attctgtgcc ggaatatgtt gtggaaacgc ccctaaataa
    11041 acaccagcag taattttgct caccggctca aggcgaagac aaaaccaacg catagacagc
    11101 cacatcccga tgcgccaggg caatgtcagc cagtaaacgg cgggcgggag tttaccctga
    11161 ctatctttcc ctgttgttat agcgccaagc ccaccgtacc cacggccaat gatgaatagc
    11221 gataatactg gccaacataa ccagactgac caccataact gaagcattat cattagctcc
    11281 atcaatacaa tgcacgagca cgcgcctacg acatagggca gtgctatttt gattcgacgt
    11341 tgatcaggtt tctgataatt ccaacgacgg tcgacgggca ccatccagtc aatcaacata
    11401 cctaccgcca gccctgtgat gacatcaata aaatgatgct gccatgtcgt cagcgtcgaa
    11461 atggcgatga gtaaaaacca tccgccgcag actttacgcc acctcacagc cagatgctga
    11521 cgaaagtgac gccaaagtag ccagcagaga ataatatgca gcgaaggaga ctggttataa
    11581 ggcagatcaa acaattcaag ttgcgaaaat aaccatcctg tcaccccact cacttcagga
    11641 cggataaaac taaacttcag cggatagaga aaaaaaccgc agcaggccat taccgttgca
    11701 agaataagcc ggtggaccag acggcgctgc tcaaatgtcg agctacaaac gaacagcgaa
    11761 aatccatata aaagatccag actccagtaa ggaaaaatag tccagggaag aaaagggatc
    11821 gccgtttccc aaccgaatac ctgactgggg atatcatggt tgttaagatc ctgagtcgcg
    11881 gtgaactgat taagaaatcc ataggtgaag aagaaaaacg gggccagcaa caataaccag
    11941 ccagctcctt gtagcaatac gttccggcgt tcagttatca cgacgcaccg ccatcgaaac
    12001 cgtaaaaatg ccccactcat caatccgttg tgtgcattta tcaaatccgg catcatgcac
    12061 gagcgaatcc atctcccctt gagaacgcac gcgcattacc cacggtttac catctttatg
    12121 actggttaac accccggcaa tcagctccag ttgtgggtgc cacggctgcc cggtgtagat
    12181 gagaatgcca cccggttcga tggcattggc aagacctgcg agtgagtttt ttacctgctc
    12241 attttcggga aaaagctcat acaggccaga gacaatcgcc agcgtaggcc gcggagttaa
    12301 cgtgctgagt tctgccggat taaacgcatc gccctgttca aaacgcgccc gcccagacat
    12361 tccccgttgg gcaatcatct cttgcccttg tgcaacattt acctcactgt aatcacgtaa
    12421 caaaatttcg cttacggcag gctcgtttgc cagcgcatcc agtacatagc gcccatgccc
    12481 tgcggcaatg tcaaccactc ggacggctaa acctttggcg tggagatggg taacggcttg
    12541 tttaatcagc atttgtaaat gggttttgcg ctggcgaata ccgcgccagc caacactgtt
    12601 caggtagatt ttgtcgataa agcgcccgaa tgcgttactg ccttgcggtt gattttgata
    12661 gacataatcc agcgaactgc ctgaatcaaa gccggtgctc attccgagat gcaggcccgc
    12721 agagtgcgcc ccgaacagtt tcatcgcctt tcgcataaag cgatacgcca aatcaaccgg
    12781 cgataacggc acgggtccac cagaaaggag ccgccatcga tccgctgatg gtcctgtgcg
    12841 gtcttcatgc tgataatcaa atttttgcga cttgttagca tataagcgac taataaagct
    12901 ttgcattttt tcaaatgcct gcgccctttt ctcttcaccc aacgtgtcgt gataaaagcc
    12961 tggcagcaga tgcagctctt tcagaggact acgtaaacgc tgataaaaat caatctgcgg
    13021 ttggcgatgc accacatagt catcgcctga tatcaaaagt tgcgtgggga gcgtaatcgc
    13081 cgccgcatct ctaataatac gttcagacgt tttgtagaga tcgagcaaga tattaacggc
    13141 aatcgcccgt gtgatcagcg gatcattatt gaaactcgcc acccgttgcc gatcgtgggt
    13201 caaatagcgt cctttcacat aggaattaat aaaaaacaga ccacgcagac gatgccataa
    13261 cgccagcgca ggacgtgcca gcggcacata caatttaacc ttaaaggccg gagaagccag
    13321 caccagcccg cgaattgcag gcgcataatc atgaacccat gtggcaacca gcactgcgcc
    13381 gacgctttgc gcgatcacta ccacctcttc cagtccgacc tggctgtcgc tggcagcaaa
    13441 acggacaaat tcatcgacat cctgcactga acgcgcaaga gacggactat aaccacgcgg
    13501 ccccgaagtt tgtccatgcc ctcgggcatc ccaggcataa aaagcagtat ctggcatcgc
    13561 cagttcatca acgagatgtt gtagacgccc ggaatgttca tgaccgcgat gaaataagac
    13621 tatgaccttt ttcgccccgg gctgtaaagt gggccagtgc cgataaaaca acgctgtatt
    13681 atcactggtg gtaaaaaaat gttccccagg gatgcgtgaa ttttccattt tctctatttt
    13741 ccttcagtaa cagcagtagc gaataaaata aaaaaatacc ggagcggtga aaattaatga
    13801 gtcaatcctg tcaaggagtc cgccatgtcc tggtaatagt tttccactat ctttaacacc
    13861 aatatctcgt ttgatggctg acatcactac atcgccgcaa aaaccactaa taccaattaa
    13921 caaacccgct aataatgcct gtaatgtatc tagcggtgtc agcaacggtc caataatcag
    13981 tgacgcaatc atggtggtga ttacgccgcc catcagacct tccaatgttt ttcccgggct
    14041 gactttaggg accacttttc ttctgccgca ggattttccc cataaatact gtgcaatatc
    14101 gtttgactcg gttaatgcca aaagaaataa caccagtaac gcctcgccct ggatatttat
    14161 ggttggcaac attaacaacc aggcggcatg actcaaggtg aaaacggtca ttattcagcc
    14221 acaaaaaatg gcactcacgg tatgcagaaa accagagggg tcacccacta aagctcgcca
    14281 ggtggctaat atcagaaacc cagccaaagg tataaataac agaaacagct caaagcaatt
    14341 aaaaccgata agcaaatagt taagaggaat accccaatat aaccaacgcg gaaaatgttc
    14401 agatataagc gtacaatatt atttcaatgc cagaaaactt atcagcgcaa aaaacgtcaa
    14461 cgtcatcccg cgtggacctg aaatcaccat tgaaaaacaa atgacgatgc cccaccaggt
    14521 acgaatacgt aatgtaacct cacccccttt tctctccggt aaccgccata tcagaaaacg
    14581 gtttatcagc gttgctaaaa ttaacaacgc gaaaagtgtt gccagagatt tttccagcat
    14641 tatctttcag cactcataaa aagcacactc cgacaacgat taatcgcagt ccagagaagc
    14701 agaattgaag caatgctcca taaaagatta ttccattgca tccactgtgg ataaatggca
    14761 acggctagcc cccacagacc aaatattaac gcgcgatcgc ttttgccaaa tggaccggca
    14821 taactgcgaa taccgttaat cgtctgcgcg agtaaaccac aaaactcggt caatatggtg
    14881 caaaataaca tgagtatcac gagtgatgca ttactttcag gtaaaaataa aaacggtaaa
    14941 tagagcgcaa tatcggaaat aacatcgcca gtctcattca aaatcgcccc aagacgtgtt
    15001 tgctgattgc actcacgcgc catcatgcca tccagcgcat tgagcgccat acggataaaa
    15061 agcacgatag gcaatagcaa aaagaggatg ggttgtgcca ccaacatcaa cagcaatccg
    15121 ctaagaagag aaagtgccag tgcagcaaga gtgatgtgat tcgctgtaac atggtgctta
    15181 ttaagccaaa acatcgtcgg ccttaacagc gactgaaaga gcggttttat ctggtagagc
    15241 gtcattatca atccttgata attcggtttg cgctatggtg catcaattcc cctgacggag
    15301 atatcgtaac acgccgcttc attaaaaatt cagaatatat caaggtattg gggactcacc
    15361 gtcaggaatg attatcccca ataaatcaag tggttaaaaa ttattacgct gttattcaat
    15421 acaactcatc aagttttctc atcgcttgtt cccgtattgc gcgcgtctta ccgttaagcg
    15481 ccagctgccg aactaaatcc agttcagggt taacaagctc taacgccagc aaaggatcac
    15541 gtttcagcct cgcctttaat atatcaactg gaaaacgggg attattcatc gccacaatcc
    15601 accactcggg atcaatttct gccgccagta ctgcaggcgg tgtgtggtgg ttgttaagtg
    15661 cgccgaactt gaaaaatgtg agattgcttt tcgccagtgt actccaccat atacgatccg
    15721 gttgttttgc cagtttatcc agttgtgcta atgcatcggg gtcaagtgca taataaacac
    15781 gtaacgcttc gttaacctgc gcacgagagc tgttttcctg cctcagaaaa aacttcgccc
    15841 aaatatcatt gcgtggtata ttttgctgta attccttgag gacaacagcg ggtaaattat
    15901 cccaactaac taatgcactc tctaaaaagt cccagccttc cgaacgtatc agagaggtgg
    15961 catttaatag catctggtca tcaacttcac cgtggctaat cagcccagac aaaatcatat
    16021 cagcgctggt ttcatatgct gcagaatctg ctttcggatt atttttccga tgcgataaag
    16081 cacgttgcca aattttttgt ttcatcgacg tgctcagata ctgatttttc gccaaattac
    16141 tccacccctc aggatcgttg aaacgtgtca ataaccgttg taaagcagcg tcagagtgaa
    16201 ttatctgcat atcccgacag caaaagtagg caggggattt tttctccatc cttttaacaa
    16261 aattaaactg taagttatct ggcaacgcta aaaataacgt acctattaga tcatcgtcat
    16321 tctcgaattt ttggtataaa tatactgcta ttttgtgact ctcttgtggc gtcatccgca
    16381 acaattgacc atggtgtttc atctctgcca gctttgacgc taagcttgtg acaacatcgt
    16441 ttgatgaatc gttcgccaac tgcattgcct gattgtagct gagttgtcct ccagcaccga
    16501 cctgacgaat agaggaatca tcactgcgga tatagccatc aataatggat tcgggcaata
    16561 accacgcatc tttataaagc tcctgaatga tcatatccca atctcttttc gccagagtca
    16621 tgacgatatc cagagggaga ttttcacgta gcgcaagatt caacggaccg acaccatcag
    16681 gtagatgact cattcgcgtc attatttcga cagagagcgg ttcacggtcg agcaacccca
    16741 gaacaacagg ctggtaaccc aattgctgat cggtcaactc cagaatctca ttgtcaggca
    16801 tatatctcgc catactttca atcgtttgtt catcattgct attaataagt tgtttgcgta
    16861 ggttagctga aattccttta agccttggga gagttcgcat tatggcaaga tcaaatgtcg
    16921 caatgtctcg cccttcaaat gcagcaactt tgccttcatc cagcatttgc aattcctgca
    16981 ctaattcagg tgaaggctta gggcgaaaca gcgcttcttt tttcatctga ttacagacat
    17041 tccctgctat gcgaatattg cttcgcttaa aaatttcttt cagagcatct tcatcattta
    17101 ttaggcccca ataatatgca tcaggattta ttatcgtcag cgcttttttg acattttcag
    17161 tgtcattttt gtctcccgca cggaccatga attcactatt tgtcgaagac acaacaacag
    17221 gtgcaattat gtcattgata aatatcccgc gcgcattaat acttccctta atcgtagacg
    17281 gattatcgta atatgagtcg ataaatacca gtccacttgc atttatcgaa ccggtaatta
    17341 acagtggtga atccgtaact atattcgttg ccgtcacgtt accgagaata acccatggat
    17401 tagaaagaga gaaagtggaa atattacctt tgactgtcaa attgccaaga acaatcaatt
    17461 tttgattact ctctatatta ccttctacca ctaaatcgcc acgatgcact ttccagtccg
    17521 atgcagataa accgaagggg acaagattca cgctactgtt gatagcttgt tcaacttgtt
    17581 gtttatcttc agcggtagcc gcagaaaaaa atggttcaat atcaactgca tttgcaacac
    17641 atcgaagttc cgcctgtact ggaagactca gcataaacag gggaagtaaa cctatccagc
    17701 tgcaaatcct gggcttaaac atatgcactc catgccatta aaaaagaaac aaaagggaaa
    17761 tgcatctttc ccttttgtat attcgttatt ctcgcgatat accatccaac gtagacaaca
    17821 cttcctcaga aagatgcaat ttttcagcag ccatattctc ccgtaaatgc gcaacagaag
    17881 acgtccctgg gatcagccaa atattcggtg aacgttgtaa cagccacgcc agcgccacct
    17941 gcatgggtgt tgcacccagg ctcgcagcaa catccgacag cgtagaggat tgcagcggtg
    18001 taaagccccc gagcgggaag aacggcacgt aagcaatgcc atcgtgggcc aaagcatcaa
    18061 tcattgcatc atcagcacgg tgcgcgatgt tgtattcgtt ttgcacacag acaatttcgg
    18121 caatcttgcg cgcctctgca acctgcgtcg gtgtgacgtt gctcaggcca atatgtttta
    18181 ccaggccttg ttgttgcatc tctgccagca cggtcaggct ggcctcaatc gatccttccg
    18241 ctgggccatg accatccccc atcataacgc gcaggttaac cacatccagt acgtccaggc
    18301 cgagattacg tagattatcg tgcaccgctt tttgcaactc tgccggagaa aaggcgggca
    18361 accaggaagc gtcctctcca cgccgcgcac caattttagt gacaattgtc aggtcgtcag
    18421 agtaaggata aagcgcttcg cggataatct gattggtgac gtgcggacca taaaagtcgc
    18481 tggtatcaat gtgattgacg cccaatgcca gcgcctcgcg cagtacagtt atagcgacgt
    18541 ggcgatctct tggggggcca aaaaccccag gacctgccag ttgcatcgcg ccataaccaa
    18601 gacggttaac ggattttgta ccgagagtaa atgtgttgct gctcatcgca ccctgccctg
    18661 taataaaacg gattaagaaa aatcagtata gaaacgaaaa gagataaata atgcgaaggc
    18721 gtaaacaaga ttactggatg agttgctcta ttgtccttga agacaccaaa acaaaacgcc
    18781 taaagcatcg catccggcaa taaaaaaccg caacctgtat cagcattgcg gtttaggtat
    18841 cacatgctaa aaattagaac gttttcttga agttcagcat cacgccttta tcgctgatgc
    18901 cgtcctcttt ccagtggtaa gcatccagat tcagcgaact ttctttgctg ctgtacttca
    18961 cgccgacgct tgccaggctg ttgatgccgc cgcccttctg accgtcatcg acattaaagt
    19021 gctgactgcc tgcgcctgcc aggcttgccg tacgctggct cttcgcgtag ctcaggttcg
    19081 gcccgccttc cagcgtagcg ctagcgctcc agccgtcttt acctgcgtaa tccagtttca
    19141 acccgacgat gctgtccacc gccgtttcgc tgccactgtt catattcagg ttaaagtctc
    19201 cggcattgcg ctcttgatag ccaccttcca gtgtgtgacg cagtttaaca cccgcatatg
    19261 gcgtaacctt cagtccttcg ctcggttcga aagtcttcgc cccttcgctg cggaactcca
    19321 ggtactgctg cttcacgtcg gtatcagccg ttttgtttgt gttgctaaat gcaatcgaac
    19381 ggctgctgtc aaggttgtga acgtcataac gcaatgcgtt attccagttc atgccgttgt
    19441 cgaaggacat ctgatgtttc aggccaaaga actggctata accgcctgta acgccattat
    19501 cacccgcttt ctgcgcaccg tcgccatcga ggcgcgcgat accgtactcc agactcatcg
    19561 tctggctttc gctcaggtcg atggttttac gcaatgccag catgtcgtac tcggtattgt
    19621 tccccagctc cgcgcgcgga tcgcctttcg caacaacgtt gaacgccaga ccgttaccca
    19681 ctttcggtgc ggcatccgcc agcatgctaa agcggttgct tagcacgcgc gcttcgcgga
    19741 ataccgtggt cgcctggcta ccgctgacct gtttcagggc actgttcagt tcggcagtcg
    19801 tgccgacgtt caggctggta tacagctcgt tgttggtgta acccgcatcc agtgctttcg
    19861 cgacgtcatt caccgaagca tcggtcgcca catcggtata agcatttttg ctcatagtga
    19921 cgtcaacgtt accgttggca tcggtgctgc ctttggcgtt ccataccacg gtcgttgagg
    19981 tgatagcatc cgcgtcggtc aggttgctgc cctccaccac gttatcaaaa ctaacggtgg
    20041 tgtctgccgt accggcggta aacccggtgt tgatccctac gccgttcatg ctggcattgt
    20101 tgaccttgag cttaccggcg ctgccatcgg cactcgtacc caccacgtaa ccgttgagat
    20161 tgttcatgcc gccatcagtt gcatcacttc ccggcgttac gctaaccgta ttgggactcg
    20221 gcacatccgg caatgtatag tcgccataat gcacttccgt gttattaccg ttgttaccgt
    20281 tcacgccttc aacattaatg ctgtcaccct gcttgatcag gccagaaccc gtaaccccat
    20341 cggcgatcac caccgtgccg ttgttaacca cgtgaccttc tgtacccagc acgctgaatg
    20401 caaacgagtc attggcgaag atattgatgg tgccgttgtt ctcgatcacc gcatccgccg
    20461 tggcgttggc atcgagttgc ataccgatca tgcccgtatc ggtagtgccc tctgtgccga
    20521 ggttgatggt gccgttgtta accagttgcg cgttcttatc accttccata gccacagcac
    20581 cggtaccgct gatattcata gtgccgtcat tctgcgcgat gaagttactg tgggttaatt
    20641 tcacagcgct ggcgctgtcg tggtcaaagt tgatgacccc gttatcctgg ttccagaaga
    20701 aagcattctg atccccagca ttactggcgc taaccaccag cgacttagca gagacatcaa
    20761 tcgttccatc gttgataaag cgaccattcc caccccacat attgacagca ccgttgtggg
    20821 aggaattact gatgtccatg tgaccagtgt tataaagcgt accggactga ttaatatgta
    20881 cagcattctt gttgttatcg aggataaact caccgctgtt attgaagacg gcgttgttat
    20941 taacttccag catacctgcg ttattgccgc cactgctact accgaggcgc caggttcctt
    21001 cgttattgac ccaggcatca ccggttaatg cagaaccatt gtccactttg ccatcttgta
    21061 agttattaaa gatgccactg gtctgaataa tacgtttact cactgtacca ctgttattaa
    21121 agacgccgga tttttgcaca acattgctaa tagtaccgga gttgttcacc agattagtgc
    21181 tagccccagc atacattgtc acattaccgt tgatggtacc ctcattattg agggtacctc
    21241 cctgagcatc aatattgccc atcgaacccg cattatctat tacagcatct ggtgccaatt
    21301 tcaccagggt gttaataatg cccgtggagg tattttcgag cgtaccgctt tgaacgttga
    21361 tattgctacc agataccgtt cctgcattaa tcgtgcttcc tggcgttgca tcagttatca
    21421 ggactgggtt aatgaagttt gtcgactctc cttcagccac cagaatatca cccccggtgt
    21481 actgtactga cacataacta tcagtcgggt tatattgttc cgggctctga caaatgccaa
    21541 aagaacagat tgtgccatag ttaacgatcg tccccgttcc atcgccgtaa aatgcctggc
    21601 cgatgtcagt atcgatatta ataacgcccg ttacgtcatt aattgctgta ccactacgga
    21661 tagctcccat cgcgaacaac tgaccttcat gttgctgcgt cagtccctca tctgagagca
    21721 gatttatttc gccctggttg atgaccgtgc caccctccat cgccagcatg ccaaagccct
    21781 ggttcttaac attaataatc ccagtattga ctatatcggc atcgccatga cccgcatcgg
    21841 tcgaacccgc taacgcgccg ccagccatca ggaagtaagc gttaaaatcc caataggctt
    21901 catcagtaat atcaccattt tcatctactg aaggaataaa accatcaaca ttgatcacac
    21961 ctgcgttagt cgcagtacca cgagaagcgg caacaccata gaccccagga ccatgaatat
    22021 taattgttgc gccggactca ttcgtcacat caccgtaatt gctggctaac agcgcaaagt
    22081 tggtgccgtg ataccataca acagggaact catacgaacc ggaggaaccc gttggtactg
    22141 ttgttgatac cagattaata ataccaccag ccccattgac tgcacgagag ccctcatcta
    22201 tagcgcgcat aacgccagct cttgcgtacg cttcagtgcc aggtttcacg ttaatggtgc
    22261 ccgtattttt tatctctgca ccagcacgcg ccatcatcaa gtcctgcgca tcggtagtat
    22321 tgataatgcc gtggttttct atttcagagc cgatacccgt tgcggtaatt agggcatcat
    22381 ccagatacca attggtatct ctcgccctga cgtcaaaatc agtgactgaa ttttcatcaa
    22441 aaatggcatg tccgccatcc gctacgctca ctaatgtgaa ggctcctctg acttgccgcg
    22501 agttcatttg tgaatgacca ctgatattca tcacgttgtt tccggtaaca gaaactccct
    22561 tgattgaatg accaccgcta tcaccagtga tatttatacc gccctctaaa ttgattgtgt
    22621 tattttcacc tgtaacatcc actccaacca gcacagcttg gtcattcaaa tagccagaac
    22681 caacattgat attaatgtta ccactcaaat cgaccgtatt gttatcaccg ccaacggata
    22741 acccagttaa actatcccca gatattgtcg gagagtctcc cataagggat acaagattga
    22801 tattaccatc caatgagata tgactatcgt ccccggacat atcaacgccg gttcctccta
    22861 caaaaacccc attactgtca cgttgctgag ttacattaat atccccgctc aaactgacgt
    22921 cttcacggtc gcctgaaatc gctgctccct ggccatcaag tgaaacattg atttccccag
    22981 tagacttaaa tactgcatca tcaccttcaa taataatgcc tacagagtta gcatcttcta
    23041 ccgtgatttt accagtatta ccgagtgtaa cccggtcacc actgatatca ataccacgtc
    23101 cggcatcact aacaaaaagg tcacctttct gacttacttt tccgccattc ccggtaacaa
    23161 gtgtcccaac ggaatcacca tcaacgacca tcgaacccgt aatttcgaga ttgttattat
    23221 cgccagatac aataaccccc gctgactgtt caccgataac attcagcgca tatgttgaca
    23281 tcgtcagggt attattgtca ccggtgattt ccagtccggt cgaaaaacca ccggtttcaa
    23341 caagcccggc aagggcgata ctggaattat caccaacaat tttaattgca gttgccgcat
    23401 tttgcgcact cacatcgccc acgttaatta acgttgagcc atcaccctcg atgagtacgc
    23461 cgattgcgtt ttcactggct gagccatcac tgacgctgat gtcccccata ttgatgaatg
    23521 cgctatcgtt accgtccaac aatataccaa tggatttttc atcaactacg gtaatattac
    23581 ctttattgct gataatggta ctattaccag tgacatcaat acccttcgca ccaccgctga
    23641 caaagaggtc accctcttgt ttcaccagtg catcattacc attaattttc gagccagtcg
    23701 aattttcacc atcaacgttc attgtacctt tattatcgac tttggcgtca tcgccattaa
    23761 tcagactacc ggtaccgcca ttggtgaatg tcgaatcacc tgcgacattc aatgtcgtac
    23821 gatcgccatt gacaataacg gccgtcccac catcaacttc cgtctcacca ttattagtaa
    23881 tggtgatatc atcaccggta agaataccta ccaccgaacc ttcaccaatc gcggtagttt
    23941 tgccgtcgtt gttcagggtg atgtcgttac cgtcaacgtt tatcacaacg ccgccattca
    24001 gggcgatgtt gccgccatgg tcggtaatag tggcatcgtt aatatggaaa tgttcgccag
    24061 ttgtaccgtc tgcggtggtg agtgactcta tgattatttc gatttttccc gcttcgtcat
    24121 agcgccaggt ggaggttccg ccaggattga cgccattgaa cgaagccgtg ttagcagcct
    24181 cgtcgactaa ccaatccttc acgattgtgg tagtgccgcc aggtccggta agcgtatagc
    24241 tgccatcgtc atttttggcg taggtaaagg tggtatcacg taattttagg gtcttcgcgt
    24301 cctgatccca ggtgacatcg tttttataga ccacaggatc gtgtttgact ggcgtgtcat
    24361 cgccaccgtc gtccggggta tcatcgccgc catcatctgg cgtgtcaccg ccgtcgtctg
    24421 gcgggactgg tgtatcgccg ccatcatccg gcgggacggg tgtatcgccg ccatcatccg
    24481 gcgggacagg tgtgtcgccg ccatcatctg gcgggacggg tgtatcaccg ccatcatccg
    24541 gcgggacggg tgtatcgccg ccatcgtccg gcgggactgg cgtgtcaccg ccatcatccg
    24601 gcgggacggg tgtatcaccg ccatcatccg gcaccggaga cggatcgtca tgatgattat
    24661 tattatcgtg gagtgcatat actgcgacgc ctgtgactgc cgctgccaac cccactgcag
    24721 cccagggtaa taactcatta tcttcaactg atttacactc tgccggcagc ttttctagct
    24781 gttctttact tagtgattta atattagccg ggcagctaat cttgctggat ttacgagatt
    24841 catccgatgt ttccgctatc gacgtatctg ccgcccatgt ctgactgctt aacgccattg
    24901 caacacagac actgagcaat tttttccttt gcatatgaaa ttcctcataa atagatgatt
    24961 acgcagccca gataaactgc acagggttaa atagttacaa atattcgaaa tctggatatt
    25021 gtttttaaca gaataaatat attgatttag c
//
LOCUS       DAPOZO010000071        24500 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPOZO010000071 DAPOZO010000000
VERSION     DAPOZO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.071"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..723)
                     /gene="mngR"
                     /locus_tag="ROI21_003158"
     CDS             complement(1..723)
                     /gene="mngR"
                     /locus_tag="ROI21_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDX5112048.1"
                     /translation="MGRKPLYRQIADRIREQIARGELKPGDALPTESALQTKFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQIIAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQSKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            893..2809
                     /gene="mngA"
                     /locus_tag="ROI21_003159"
     CDS             893..2809
                     /gene="mngA"
                     /locus_tag="ROI21_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDX5112049.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLERSGETRTVQQDT
                     QPAKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGMLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            2827..5460
                     /gene="mngB"
                     /locus_tag="ROI21_003160"
     CDS             2827..5460
                     /gene="mngB"
                     /locus_tag="ROI21_003160"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDX5112050.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILRRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKERVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIETNQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YDMAVPRNLQERSARQCTGRVGVELVVTLSDNSRRIDVVINLDNQADDHRLRVLVPTP
                     FNTDNVLADTQFGSLTRPVSDSAMDVWQQEGWKEAPVPVWNMLNYAALQEGRNGLAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGSFSC
                     RLSLFSYTGTPSTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVVLRLFNPAESTTCEATVAFSREVISCTQTMMDESISTAERD
                     NLKELGAFLPGQSRTFSYRLG"
     gene            complement(5604..7001)
                     /locus_tag="ROI21_003161"
     CDS             complement(5604..7001)
                     /locus_tag="ROI21_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HDX5112051.1"
                     /translation="MHHLSRLDKAFLRKGIQLSRANNIVSLSRPSGASARVLLPDELP
                     LEEKAVTQLLDFAEAKLPDHPGYVRQTCATPDFHPGNGVPVGAVVASTPDLVIPAAIG
                     TDISCGMRLLTTGLTLEQAEAHKDVIAQRLTQVLLQNERDVPLSAQAFTALFDGGPAD
                     CVSTLPSAGLWTRTNIPRMVQEIDRCIGLNEHNSHSRYAPPALLATDRALLRDPCLGT
                     PGSGNHFIELQVVDSVVDRHVAWQQGLKTGDIVVMIHSGSRDVGFYVGRRWMDRARAE
                     WPTGHKHPQSGLYGLAGGLAEEYLQAIGMAARYAWLNRVTIAEMVRVCLANLFHQDNS
                     HLVVDVPHNIILREHEMNIHRKGATPARVGDLALIPGSMGDYSWLAVGCGNPEWLWSC
                     SHGAGRSQRRQAMRSSATEESTLPWQCVTLREERRLEEAPAAYKDIGPVIEAQQEAGL
                     IQPAVRFRPWLTFKG"
     gene            7591..8496
                     /locus_tag="ROI21_003162"
     CDS             7591..8496
                     /locus_tag="ROI21_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5112052.1"
                     /translation="MRGKIPKTELLVTFEVVARYESYTRAAEELALTQSAVFRQVSAL
                     EEFLHTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFDEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(8530..9132)
                     /gene="cobO"
                     /locus_tag="ROI21_003163"
     CDS             complement(8530..9132)
                     /gene="cobO"
                     /locus_tag="ROI21_003163"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDX5112053.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRIAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(9142..10794)
                     /locus_tag="ROI21_003164"
     CDS             complement(9142..10794)
                     /locus_tag="ROI21_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDX5112054.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGILP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGNEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAPHVQLDLNRPLRDVMQDLARLPVGTRVSLNGPIVVAR
                     DIAHAKIKARLDSGEPMPDYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(10902..12182)
                     /locus_tag="ROI21_003165"
     CDS             complement(10902..12182)
                     /locus_tag="ROI21_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDX5112055.1"
                     /translation="MKKISLTTMILLALVLGVIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVASTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(12343..12660)
                     /locus_tag="ROI21_003166"
     CDS             complement(12343..12660)
                     /locus_tag="ROI21_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HDX5112056.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(12657..14027)
                     /locus_tag="ROI21_003167"
     CDS             complement(12657..14027)
                     /locus_tag="ROI21_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HDX5112057.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQQALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDSEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIHINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(14031..15272)
                     /locus_tag="ROI21_003168"
     CDS             complement(14031..15272)
                     /locus_tag="ROI21_003168"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HDX5112058.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDTFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKLVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHNMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(15272..16717)
                     /locus_tag="ROI21_003169"
     CDS             complement(15272..16717)
                     /locus_tag="ROI21_003169"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HDX5112059.1"
                     /translation="MELRNKKLTHNEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     NYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARFEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(16736..18124)
                     /locus_tag="ROI21_003170"
     CDS             complement(16736..18124)
                     /locus_tag="ROI21_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDX5112060.1"
                     /translation="MQIVSVDIGSTWTKAALFAREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNLVLNVDNALPLFNSGEVTLKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(18124..18573)
                     /locus_tag="ROI21_003171"
     CDS             complement(18124..18573)
                     /locus_tag="ROI21_003171"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HDX5112061.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGNILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFSPSHDLEDVCQLMAHDINQRHNVDTRILEEAI"
     gene            complement(19120..19542)
                     /locus_tag="ROI21_003172"
     CDS             complement(19120..19542)
                     /locus_tag="ROI21_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112062.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFPILSSLIDSAISKFQHQIPVNGSVNPDLLYEDYTD"
     gene            complement(19623..20567)
                     /locus_tag="ROI21_003173"
     CDS             complement(19623..20567)
                     /locus_tag="ROI21_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112063.1"
                     /translation="MSPDNISSAVNRFSGWPSNDPLKTDKESMTSPMTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDVLLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
     gene            21373..22941
                     /gene="cydA"
                     /locus_tag="ROI21_003174"
     CDS             21373..22941
                     /gene="cydA"
                     /locus_tag="ROI21_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDX5112064.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            22957..24096
                     /gene="cydB"
                     /locus_tag="ROI21_003175"
     CDS             22957..24096
                     /gene="cydB"
                     /locus_tag="ROI21_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDX5112065.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            24111..24224
                     /gene="cydX"
                     /locus_tag="ROI21_003176"
     CDS             24111..24224
                     /gene="cydX"
                     /locus_tag="ROI21_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDX5112066.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            24224..>24500
                     /gene="ybgE"
                     /locus_tag="ROI21_003177"
     CDS             24224..>24500
                     /gene="ybgE"
                     /locus_tag="ROI21_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDX5112067.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGL"
BASE COUNT         5956 a         5796 c         6183 g         6565 t
ORIGIN      
        1 ttatcgcgat gattttcgct gcgctatcag ggtaaattta tagtcatcag tattaaaagc
       61 gttgcggcta tattcaaaca cccggccatc aactaaatat ccacgcgata ctttttcaag
      121 aatcggctta gactggctga tattaagcag acggctcatc tcttcggttg gcatcagagg
      181 aatgatttcc tgttcgctac gatcgataac cattttcttc acttcttcaa taaagtgata
      241 tttcgaattt tccataacct gccaggtgag atccgggaac aacgcaagcg gcatccaggt
      301 ttcttccagc gccattggct tttgcttgcg atagcgcacg cgcttcacat gccacacacg
      361 atcctgcgcg atgatttgta gctgttgctg aagaaaatcg tcagccggaa tcacttcaaa
      421 tatcagaact tcactgtgtg tatcgacgtg acggtccgac agtttttcat caaaactggt
      481 taactgaaaa atatcgtaat tgacccgctc ttctttgacg taagtcccgc tgccctgaat
      541 gctttcgagg atctgttgct cgactagctg gcgcaaagcc tgacgcaccg taacccggct
      601 gacgccaaac tttgtttgca atgccgattc ggtaggtaac gcatcgccag gttttagctc
      661 gccacgcgca atttgctcac gaatgcgatc ggcaatctgt cgatataagg gtttccgtcc
      721 cattatttag catctcatta atacgaattt agccattatg cacgataaat tcatcctgta
      781 aataatacaa atacaataca aataatttca atcaagtgaa attgatcaca taatagtatt
      841 gttttatcgg gcacactggc gcgactataa aaacgatcaa gtgaggatca tgatgaacct
      901 gacgactctg acccaccgcg atgcgttgtg tctgaatgcg cgctttacca gccgtgaaga
      961 ggccatccac gcgttgactc aacgtcttgc tgctctgggg aaaatttcca gtactgagca
     1021 atttctggaa gaagtgtatc gccgtgaaag ccttggcccg accgccttag gtgaagggtt
     1081 ggctgtgccg catggtaaaa ctgctgcggt aaaagaagca gcgtttgccg ttgcgacact
     1141 gagtgagccg cttcagtggg aaggcgtgga tggcccggaa gcggttgatt tagtggtgtt
     1201 gctggcgatt ccccccaatg aagcgggtac tacgcacatg caactgctga cagcgctgac
     1261 cacgcgcctg gcggatgatg agatacgggc gcgtatacag tcggcgacga cgcctgatga
     1321 attgctttcg gcgctggatg acaagggagg cacgcaaccc tcagcttctt tttccaacgc
     1381 gccaactatc gtctgcgtaa cggcctgccc ggcgggtatt gctcacacct atatggctgc
     1441 ggaatatctg gaaaaagccg gacgcaaact cggcgtgaat gtctacgttg aaaaacaagg
     1501 cgctaacggt attgaagggc gtttaacggc agagcaactc aatagtgcaa ccgcctgtat
     1561 ttttgcggct gaagtcgcca tcaaggagag tgagcgtttt aacggcattc ccgcgctttc
     1621 agtgcctgtt gccgagccga ttcgccatgc tgaagcgttg atccaacaag cactgaccct
     1681 cgagcgtagc ggtgagacgc gtaccgtaca gcaagatacg caaccggcga aaagcgtcaa
     1741 aacggagctg aaacaggcgc tgttaagcgg gatctctttc gccgtgccgt tgattgtcgc
     1801 ggggggtacg gtgctggcgg ttgcggtatt actgtcgcaa atcttcgggc tacaagatct
     1861 atttaatgaa gaaaactcct ggttgtggat gtaccgcaaa ctgggcggcg ggatgctcgg
     1921 gattttgatg gtgccggtgc tggcagccta taccgcctat tcgctggcag ataaaccggc
     1981 gttagcgccg ggctttgcgg ctggacttgc cgccaacatg atcggttccg ggtttctcgg
     2041 cgcggtcgtt ggcggattga tagccggtta tctgatgcgc tgggtgaaaa atcacttacg
     2101 tcttagcagt aaattcaatg gattcctgac tttttatctc tacccggtgc tcggtacgtt
     2161 gggagcgggc agtctgatgc tgtttgtggt gggggaacct gtcgcctgga tcaataactc
     2221 gcttaccgcc tggctgaacg gtctgtcagg aagtaacgcg ctgttgctgg gtgccattct
     2281 cggttttatg tgttcctttg accttggagg gccagtgaac aaagccgctt atgcattctg
     2341 cctgggcgca atggcgaacg gcgtttacgg cccgtatgcc attttcgcct ccgtcaaaat
     2401 ggtttcggca tttaccgtaa ccgcttccac gatgctcgca ccgcgcctgt ttaaagagtt
     2461 tgaaattgag accgggaaat ccacctggct gttagggctg gcaggtatta ccgaaggggc
     2521 gatcccgatg gcgattgaag atccgctgcg ggttattggt tcgtttgtgc tgggctctat
     2581 ggtaacgggc gctattgtcg gcgcgatgaa tatcggcctt tcgacacccg gtgccggcat
     2641 tttctcgctc tttttacttc atgataatgg cgcgggcggt gttatggcgg caattggctg
     2701 gtttggtgcg gcattggtgg gggctgcaat ctcgactgca attctcctga tctggcggcg
     2761 tcacgcggtt aagcatggca actatctgac tgatggcgta atgccataaa caaaaaggaa
     2821 acgacgatga aagcggtatc tcgcgttcac atcaccccgc atatgcactg ggatcgggag
     2881 tggtatttca ccaccgaaga gtcacgcatt ctgctggtca ataatatgga agagatcctg
     2941 cgccgactgg aacaggacaa cgaatacaaa tattacgtac tcgacgggca aacggcgatc
     3001 ctcgaagatt atttcgcggt gaaaccggaa aacaaagagc gcgtgaagaa actggtagaa
     3061 gccggtaagt tgattatcgg cccctggtat acccagaccg ataccacgat tgtttctgcg
     3121 gaatccatcg tccgtaatct gatgtacgga atgcgtgact gcctcgcgtt tggcgagccg
     3181 atgaaaatag gttatttacc agattccttt ggcatgtccg ggcaactgcc gcatatttac
     3241 aatggatttg gcattacccg caccatgttc tggcgcggat gttctgagcg ccacggtact
     3301 gataaaaccg agtttttgtg gcaaagcagt gatggtagcg aagtgacggc gcaggtgctg
     3361 ccgctgggct acgccatcgg taagtactta cctgccgacg aagacggatt acgcaaacgt
     3421 ctcgacagtt attttgacgt gctggaaaaa gcgtcagtaa ccaaagaaat tttattgccg
     3481 aatggtcatg atcagatgcc attgcagcaa aatatcttcg aagtgatgga gaaacttcgc
     3541 gaaatttatc ctcaacgaaa gtttgtgatg agccgctttg aagaggtatt cgagcagatt
     3601 gaagcgcagc gagataatct ggcgaccctc aaaggtgaat ttatcgatgg caaatatatg
     3661 cgcgtgcatc gcaccatcgg ttccacgcgc atggatatca aaattgccca cgcgcgtatt
     3721 gaaaataaga ttgttaatct gctggaaccg ctggcaacac tggcctggac tttaggtttt
     3781 gaataccacc acggcttgct ggagaaaatg tggaaagaga tcttaaaaaa tcatgcccac
     3841 gacagtatcg gttgttgttg cagtgacaaa gttcatcgcg aaatcgtcgc ccgcttcgaa
     3901 ctggctgaag acatggcgga taatctgatt cgtttctaca tgcgcaaaat tgccgataac
     3961 atgccacaga gtgatgccga caaacttgtg ctgtttaacc tgatgccctg gccgcgggaa
     4021 gaagtgatca acaccactgt gcggctgcgc gctagccagt ttaatttgcg ggacgatcgc
     4081 ggtcagcctg taccgtattt tattcgccat gcccgtgaga tcgatcctgg cctaatcgat
     4141 cggcaaatcg ttcattacgg taattacgat ccctttatgg agtttgatat tcagatcaac
     4201 cagattgtcc cttctatggg ctatcgcacg ctttatatcg aaacgaatca gcctggcaac
     4261 gtgattgcgg caaaaagtga cgctgaaggg atactggaaa atgctttctg gcaaattgcg
     4321 ctcaatgagg atggaacgct acaactggta gataaagaca gcggtgtgcg ctatgaccgg
     4381 gtattgcaaa ttgaagaaag ctctgatgat ggtgatgaat atgactattc acctgcaaaa
     4441 gaagagtggg taattacttc ggcgaatgcg aaaccgcaat gcgagattat tcatgaggcc
     4501 tggcagagca gggctgttat ccgctatgac atggcggtgc cgcgtaatct acaagaacgc
     4561 agcgcacggc aatgcacggg tagagtaggg gtggagttgg ttgtcactct gagtgataac
     4621 agcaggcgca ttgatgtggt tatcaacctt gataaccagg ctgacgatca tcgccttcgc
     4681 gtcctggtcc ctacaccttt caacaccgac aatgttctgg ccgataccca gtttggttcg
     4741 ctaacgcgcc cggtgagcga cagtgcgatg gatgtctggc aacaagaagg ctggaaagaa
     4801 gcgcctgttc cggtgtggaa tatgctcaac tatgctgcct tacaggaagg gcgtaacggt
     4861 ctggctgtct ttagcgaagg gctacgtgaa ttcgaagtca tcggtgaaga gaagaaaacc
     4921 tttgccatta cgttgctgcg tggcgtgggc ttactgggga aagaagatct gcttttaagg
     4981 cctggacgtc cttcgggtat aaaaatgccg gtgcccgatt cacaattacg tggctccttt
     5041 agctgccgtc tgagcttatt tagttataca ggtacgcctt cgaccgccgg tgtagcccag
     5101 caggcgcgtg catggctgac gccagtacag tgttacaaca aaatcccgtg ggatgcgatg
     5161 aagttaaata aagccggatt caatgtgccg gaaagctata gcttgctgaa aatgccccct
     5221 gtgggatgcc tgattagcgc tcttaaaaaa gcggaagacc ggcaagaagt ggtattgcga
     5281 ctgtttaatc cggcagaatc gacaacctgt gaggcaactg tcgcgttcag tcgtgaggtg
     5341 atttcatgta cgcaaacgat gatggatgaa agcatcagca cagcggaaag agataattta
     5401 aaggaactgg gggcattttt accggggcag tcacggacat ttagctaccg ccttggctga
     5461 ttattgcgct aaaagtgtat ctaagggggc aataatttca ggccatcttc ggatggcctg
     5521 agattgttga taaacaagtt tgttagctta cgcagatacc agatttgacc tgcgtgagaa
     5581 ccgcaggcca tttatcattt cagttacccc ttaaacgtta accacggtcg aaatctgacc
     5641 gctggctgaa ttaatccggc ttcttgttgg gcttcgataa ccggaccaat atctttataa
     5701 gccgctggcg cttcttcaag acgtcgttct tcacgcaatg tgacgcattg ccacggcagc
     5761 gttgactctt cagtcgcgct tgagcgcatg gcctgtcgac gctggctgcg tcctgctccg
     5821 tgactgcatg accacagcca ctccgggttg ccgcaaccga ctgcaagcca cgaataatcg
     5881 cccattgaac cggggattaa tgccagatcg cccactcgtg ccggtgttgc acctttgcga
     5941 tgaatattca tttcatgctc gcgaaggatg atgttatgtg gtacatccac caccagatgc
     6001 gaattgtcct gatgaaagag attggcgaga catacgcgca ccatttcggc aattgtgacg
     6061 cgatttaacc acgcgtaacg cgccgccatt cctatcgctt gtagatattc ctccgccagt
     6121 ccgcctgcca gaccatataa cccgctttgt ggatgtttat gtccggtggg ccattcagca
     6181 cgggcgcgat ccatccagcg tcttccgacg taaaagccaa catctcgcga tccactgtgg
     6241 atcatcacca caatatcgcc tgttttcagc ccttgttgcc aggctacatg gcgatcaacc
     6301 acgctatcaa cgacctgaag ttcaataaaa tgattacctg aacccggcgt acccagacaa
     6361 ggatcgcgga gtaatgcgcg gtccgttgcc agcagggcgg gtggggcata acgcgaatga
     6421 ctgttatgct cgttaagccc aatgcagcgg tcaatctctt gtaccattcg ggggatattc
     6481 gtgcgtgtcc agagacctgc tgatggcaag gtcgacacgc aatctgccgg tccgccatca
     6541 aaaagcgcgg tgaacgcctg tgcggataac ggaacatcgc gctcattttg cagtagcact
     6601 tgcgttaacc gctgagcgat gacgtcttta tgcgcttcgg cttgctcaag tgttaatccg
     6661 gtcgtcagca gccgcatgcc acaagaaata tcggtgccga tggcggcggg aatgactaaa
     6721 tccggcgttg aggccaccac cgcgccaacg gggacgccat tgccgggatg aaaatctggc
     6781 gtcgcgcacg tctggcgcac ataacctggg tggtctggta gcttcgcttc tgcaaaatca
     6841 agcagttgtg ttactgcttt ttcttccagc ggcagttcgt ccggcagcag tacgcgggca
     6901 ctggcacctg atggacgaga taatgaaaca atattgttgg cgcgggaaag ttggatccct
     6961 ttgcgcagaa aggctttatc aagccttgaa agatgatgca taacgctgtc ctgaaatata
     7021 gacgttaaac aaccccctgt attgcgaggg taattttgtc tttccggtca gcagccagca
     7081 aaagggaaag aaaacgcgcg tagtgtatgt ggcgatagca agttttacaa gtggtaaatc
     7141 tggcttattg ttaaagagga attatctttt gtgaagtctt ttaaggccat gactcattca
     7201 atttaaccgg acatagataa tatataacat agggtatata aatttatcat tgtatacttg
     7261 tcataattca agttgcatgt gctgcatcga tgttcgctca ctcaggtcac ttacttatgt
     7321 aagcttctgg aaattcatta tttgttccct tcctgcaact tgacttattt agagtatatt
     7381 catgtatatg aataatattc ttgcaataat agctttaaag tattgcatgc ggataactga
     7441 ggtgaaattc ctgttttagg attattgtca ctttattttc ctggttagac atatagctga
     7501 ctggtttgtt tatttgaatt atgatattca ttagtgctac tgttttttat aaaataattt
     7561 ttgaattgtt attttttcac ggacggaatt atgcgaggaa aaatacctaa aacggagctg
     7621 ctggtgactt ttgaggtcgt cgcacgttat gaaagttata ccagagcggc agaggagtta
     7681 gcgttaaccc aaagtgcagt attcagacaa gtaagtgcgc tggaggaatt tttacatact
     7741 ccgttatttt cacactctaa aaaacgcatt tttcttaatg atgcagggaa gtattacctg
     7801 gggatagtaa aagaaacatt aaataagctg gaacgcgata ccaataccat tatgacctgg
     7861 cagcctacgg ttcaggttat tgaacttgcc gtgaatccta cgtttagtac ccactggtta
     7921 atcccaaatt tacatgaatt cacaaagtta catcccgata ttattgttaa tattcactct
     7981 ttagctaata acggcgattt tcttaatcgg gagtacgatg ctgtcattat gcgcgaaaat
     8041 ttttgcgcgc cctgggcgga agtagaatat ctctttgatg aggagatttt gccggtatgc
     8101 agcggcagtt tgcttgcaat gtccgatcaa aaactgtcag tggcagaact gcttacagag
     8161 ttaccacttc tgcatcaaag tacccgtatt actggctggg aagagtggtt tgccttatct
     8221 ggcgttagca gtccgctggt caacaacgga ccacgtttcg atttgctttc aatgctgatt
     8281 gccgccgtgc gctctaatct cggcgtggcg ctgttaccac ggttcgctat tcaacatgat
     8341 ttggatagtg gcgatatggt gattccctgt gatgtgccta ttcgtaccgg taatcgtttt
     8401 attatgacgt ggcaggaaga gaagtctgac tcaccgcact tgcagcaatt tcgtgaatgg
     8461 ttgctggcga aatcggttgt accgcaggag atgtaaaacc tgcgcctggc gggatgccag
     8521 gcgcagttat taccagtcga tacccggctg ggcctgaatg ccgttatcaa aagcatgttt
     8581 gacggggcgg atttcgctga ccgtatccgc catttttaat atctgactgt ggcaaccgcg
     8641 tccggtgaca atgacacttt gttgtgccgg gcggttttgc agagaagcga tcacttcttc
     8701 ggtatccaga taatggtaag ccagcatata ggtcagctca tcaagcacca ctaagtcgta
     8761 acgcttgtca gccagcatcc gtttgctttc cgaccagacc tctttcgccg cgtcgatatc
     8821 cgcttgccgg ttctgtgtct cccaggtaaa accggttccc ataatatgaa actcaacccc
     8881 cagcggctgg agaaggttgt attcgccgtt gtcccattgc cccttgatgt actgcgcaac
     8941 ccccaccgtt ttgccatgac ccaccgcgcg cgtcacagta ccaaacgcgg cagtggattt
     9001 ccctttgcca ttgccggtga aaacaatcaa aatccctttt ttctctatcg ccgccgcgat
     9061 gcgcgtatcg acctgttcct tcaatttctg ctggcgctgc acatgccgtt cagttgaaat
     9121 tcgcgcttcc attcattttc cttaatgtcc cgcagggcaa ctggcgcagc ggtgttgctg
     9181 ctcaaactga ctaaagaaat tattgccttt atcatctacc aggatgaatg cgggcagatt
     9241 ttccacttcc atcatccaca ccgcttccat acccagctcc gggtattcca ggcaacgcag
     9301 acttttgaca tattcttgcg ccagtaacgc agctgcaccg ccaatactac ccagattgaa
     9361 gccaccatgt ttgtgacagg catcggtgac ctgctggctg cggttacctt tcgataacat
     9421 caccagactg ccgccagccg cctggaaggt atcgacataa ccgtccattc gaccgcccgt
     9481 ggttggaccc agtgaaccgc aggccatgtt ctccggtgtt ttcgccgggc cagcgtaata
     9541 gacaatatga tgcttcaggt aatccggcat cggttcgccg ctatccagcc gcgcttttat
     9601 tttggcgtgg gcgatatcgc gcgccactac gatgggacca tttaacgaca cgcgggtgcc
     9661 taccggtaat ctggcgagat cctgcatcac gtcacgcaac ggacggttta gatccagttg
     9721 tacatgtggc gcgtgatttt cttcccgcag tgaggctgga atatattgcc ccggattgtg
     9781 ttcgagtttt tccagccaga taccgtgctt gttaattttt gctttgatat tgcgatcggc
     9841 agaacaggag agcgccatag ctatcgggca agagccaccg tgacgcggca gacgaatgac
     9901 gcgaatatca tgagcgaaat atttaccccc aaattgcgca ccaataccaa attgctgact
     9961 ggcctcgagc aacacttttt ccagctcaat atcacggaat gcttgtccct gctcgtttcc
    10021 gctggtgggc aaattgtcgt aatatttcgt tgaggccagc ttggcaactt ttaaggtttg
    10081 atcggctgaa agccctccga cgacaaaggc gatgtggtaa ggcgggcagg cagcggtgcc
    10141 cagcgatttc attttttcga ttaaaaatgc ggtcagtttt tctggttgca ataaggattt
    10201 ggtttcctga taaagtgcgg ctttattggc ggagccgccc cctttattaa cacagagaaa
    10261 atggtactca ttaccagcga cagcgctgat atcaatctgc gccgggagat tggtttgcgt
    10321 attgacttcg gtatacatat ccagcggcgc attttgtgaa aagcgcagat tattttcctg
    10381 gaaagtactg taaatgcctt tgcttaaggc ttcagcgtcg ttacctcccg tccatatctg
    10441 ttgcccttta ctggcaacaa tagttgcggt gccggtatcc tgacagttgg gtaatatccc
    10501 ttttgctgag acttcagcat tacgtaataa ctgcaacgca acgtatttat cattactact
    10561 ggcctgcgga tcgttaagaa tgctggcgac ctgttgcaaa tgagcggaac gtaagaaaaa
    10621 agaggcttca taaaatgcct gccgggcaag cagcgttaat gcttcaggag cgatttttat
    10681 cacttcttcg ccgtctaatt ccgttactgt aatatgttga tcgctaatta atgtatattc
    10741 tgtaccgtct ttattttgta gaaacggttc ttgccagata aatggtttgg acatatttca
    10801 cttttcacct taaacgttag tgttacttaa ttgccgtcta acttcatgat ccggcttata
    10861 aaaatgcgta gtcattggct tctgattgct taagatcccc ctcagggcag agcaacgttc
    10921 gattcattgg gttttaatgc tttaccccga caaccctcgc cgaaatgttc gccttcccac
    10981 cggctcacca tcgcagcgcc cattgcgttg ctcatgacgt ttgtcgcgga acgccccata
    11041 tcaaggaacg gatcaacgcc catcagcaag atcagccccg cttccggaat attgaactgg
    11101 ttgagcgtgg cggcgataac caccattgag gcgcgtggta cgccagccat tcctttcgag
    11161 gtcaacatca ggatcaacag catggtgatt tgctcaccga tggataaatg gatattgcag
    11221 gcctgggcga tgaaaactgt ggcgaaggag cagtaggcca ttgatccaac gagattaaat
    11281 gagtagccaa tgggtaagac aaagctggca attttggggg aaacgccaaa ttgctccagt
    11341 ttttcaagcg ttcccggaaa agccgcttca gaactggatg tggtaaatgc cagcagggcg
    11401 ggttccgaaa gggcgcgggt caggcgtctg atgcaggggc cgacataaac gatggccaga
    11461 ccgataagca gcacccaaag taataacatg gtgaaataaa attcacccat aaagatcccg
    11521 gcgctcacca taactgccag tcctcgttca gcaatcaatg ctgaaatagc agcgaatacg
    11581 gtcaggggag cgaagagcat gacgtagcca gtgagcttta acatggcatg tgccagcgaa
    11641 tctaaggcgt gaacgatggc gctgcctttc tcgccaatcg ccgtcaggct acagccgagg
    11701 aaaattgaga acaccacgat ttgcaaaatt tcattgtgcg ccatggcatc gacaatgctg
    11761 gtggggaaag catgcgaaat aaatactttt agcgtaaagg gttctgacgc gaccactccg
    11821 gtggtttcgg ctccgtgtgc aacaaaatta attcctgtgc ctggcatgaa gaaatttact
    11881 gttatcaagc ctaaggcaat tgacagcaat gaggcgcaaa taaataaaaa gagtgtttta
    11941 gaaaaaatac gaccaagggc tttggcatct cccattttag ctatacctac caccagggta
    12001 gaggccacta acggagcgat aatcattttt atcagtcgta agaaaatcgt cgtgaatatc
    12061 gatatttctt gcgcatagag ttttgcggtt tccggtgaag cagtgttatt gagcactacg
    12121 ccgataatca ctccaagtac cagcgccaaa agaatcatcg tggttaaact tattttcttc
    12181 attttttcct ccatcaggtg cacggcgata aatcagaatc aggagaagta gatgcctgaa
    12241 tagagattca ggcacctacc tgctattaca accttacaaa tagctgaagt gttgaactgc
    12301 aataagtgac gggatgtcag ttggctaaaa gtattcggat ttttacaaat cgaaactcat
    12361 atttaataaa ggtgcatgtt gctgtgcacc atagacatca ttgtcaccta cattcccgga
    12421 gataatatct cgtggcatga cgatttttac tgcgttagaa ggatcaaacc agacaatacg
    12481 gtgaataaag tcaggttcaa catgatataa gcgcgcaatc agttctgggg ttaattgccc
    12541 tgatgattta actcgctcat agttttcttt cgttttaaat aaaatatcta aaaccagttc
    12601 gtaaggtccg gcatttttcg aacgaatgac ttgcgccagt gagacaattg actgtttcat
    12661 gcctgaactc cttctggcgt cacctgttca atatggaaat caaaacgcag agcgtcgttg
    12721 gcttcaatca ggtgatatat ggaaaattcg tatactggcc cgccctggat atcagaaggg
    12781 gagaacggga acgccaggtt gcctgcggta gcgatgcggt tttcatagcc gtagtgcagc
    12841 atagtagagc gcaccagcga gcaaacgctg ttggcaatct cctgggtcgg cgcaactaca
    12901 tctaggacaa tccccagctc atgcccggca gtttgcatcg gttcatggtc gcccatcacc
    12961 ccgtttttgc cgtacaggtg gaaattgatg tgaatgctgt catcgtcgag cgacaggtta
    13021 cgcgaaacgc tggttttgac ttcatcgatg attttatcga tcccggcgat catgatcggg
    13081 tcgcgcgttc cggcgatggt cagacagcgg aagcccaccc gtcgcgcacc ttccagtttc
    13141 agggcatacg gcgtttcttc atgcttagaa ccgctgacgt acacttcgct gtcattgacc
    13201 gctttaaatg tgcagccttt caggttcaac acgccgccag gtccaggcag gaagtaggga
    13261 tcggatttct catacagtgt gtgtgcagcc gctgacgttt cggtaaattt acgcttcgga
    13321 ttaaatgtct tcagcgtaaa gccgttgtca tcaatgatgc ccatcgcaca gtcagagcct
    13381 gagccgggcg ttgcggcgat tgccgcgcat tcaaggattt tcccacaatg cagcgccaga
    13441 ccttcatcaa agccttgcat gatcggaagc gcagcaaagc aggccgggtc gtaagcgcgc
    13501 ccgcccagca ccacttgcgc gccttctttc agcgcctgct ggaagggttc gatgcccatt
    13561 tgcgcgacga tatacgtact ttcgtcaatc gcgtcgtggg ttaacggcgg gacaaaatcc
    13621 agcgccgtga ttttgccgtt atccagcgcc tggtggacga tggctttgtc aacgtctgcc
    13681 gggatcagcg ccatcgagaa cgagaggtgc tcttcccggg caatctcatg aattatttct
    13741 cggcaccact ccaggtgtgg tgcggcacca gaaccgccag ccgttccgat caccaccggt
    13801 atattatttt gcacgcctgc ggtgatcata tagcgcagat cgcgcttgac ccctgcgcga
    13861 tcggtaaacg gtttgcctgc cccgaggtag tgggggcctg gatcggaaga gcctgcatca
    13921 acggcgatca gatcgggcga ttctgccatc gctttgcgga agctctcttc cgggaatcca
    13981 tacccgagga ttgccgttgg cgataagact ttaaaagaac gagccatttg ttaatcctta
    14041 gcctgcaaca gcgcgatagt acgattcatt tcgttgaaga cgatatcgag gccttcgtca
    14101 aagcccatcc ctggttttac cagcatacgc atggggcgag cggcaagggc gacgtggaca
    14161 caggtgcggg cactgacatc agtttcattg caggtgccgc cctggtacgc ttccatgttg
    14221 tggctgttgc aataaagaac tgcatcgacg atgttgtgaa tactgcccag atccggggtt
    14281 ttgatttgca ccatgtggca actggcggca tcggtgaaat caacaatatc ctggtaggtg
    14341 ttacaccatt catcggccac tagtttcacg ccggaaccga ggcgcgtcag ctctttggta
    14401 atcgcggtca gcaggcgaat ttgatcgggc ttgttaccgg catcgaccgg accttcaatg
    14461 tagagcggca ggccttgcgc ttctttttcc aggctggcga tgtattgcgc acagcgaagc
    14521 ggatccatat cgaagatcag accgatagtg ccgtatacat cgatgtgcag ggtagggtgg
    14581 tagcgtgcgc tggtgcgctt gctcagaata cgatccgaca gccagcggac atattcgcgc
    14641 agtttttcac ctttaaagcc cagtttctct tcgacgttat taatcagtgc atggggcagc
    14701 acgtcgatgc ccttaaggat catcttatcg acggcgatat atcgatcgtc gccgctctga
    14761 ccaaataatg gaatggattc cgccacgcgt ggcaactgcc attcatcaca gaccacttca
    14821 gttttcagac ggccggttgc cagcgcggta gcatcaagca gcgcctgtga taatccgtag
    14881 cgcacggcgg tatgcagcaa gttgccgtca atacgcaatt tgtcgaagaa acgggcattc
    14941 ggcaggaaag tatccacatc gcggcctacc agtaatggct tgatatggtc gttgaggaac
    15001 ggaataaagt gctccgcgag gaacagtgga tcgcgaccac ctgccccgga atactgtacg
    15061 gcagcacagt cacctacggc gaccgcgccg ttttccagaa tcaactgtac cgatacgcac
    15121 tccccggcct gacgtacggc gttaaacccc tgtgttactg gctccccggt ataaaaaaaa
    15181 ccgtcgtgac ccgctccgtt ttttatcgcc tgctggtcat cgaaatagaa tgaggagtag
    15241 ccagcggtaa aaagggcctg ttttattttc attatggtct tcctattaat ttactgtggg
    15301 atacagcatt gatgtcatct acaaccatct ggaatgtcgg ttggcgtcct tcaaagcgtg
    15361 cgcgttccgc aacgtaatcg tggtggaggt cgagaatatc ttttggcaat ggaacggctc
    15421 cggcttccag tacgcgtatc gcgccggtgt tgtcacggac cggaagaatt ttgcccgcgt
    15481 tacacgcggc tggtgcaaat ggcacatcca gtacgcccgc ttcaaatgcc agcaccgtac
    15541 cgcgcgcaat atcaccatta cccagttcga acactttatt cagtaccgcg cgaacctcgc
    15601 tcttaatcag ttcaatttcc agttcaaccg cagggcacgg cgggaatttc tgctcattga
    15661 ccatgttgag catctggcgg gaggctttca gcccctgaat gttggcagcg gcagtcggga
    15721 ttccccaggc ttcgtgaggg cttttggtga tgactttggt ggcaccagac atccccgcga
    15781 cggctgcacc ccaggagata atggcgaacg ctttcgattc gtcttccgga aagccgccca
    15841 tccactggtg aaatacggta cttaattcat agtcggtata gccatagttc tgaaagtatt
    15901 cgtgtgcaag ttcacgcaga gactggatcg ccgcgacatc ctgcgtcagg ctgccgacct
    15961 gtccataacc aacggtgata gatttcacac cctgttccag cgccagtaat ccttcgatta
    16021 tcgcgataga gtgcgagata aacggcggga tcagcgtacc cgttagtggg ccaaatggtt
    16081 cgcggttgat gcggatcccg tgttcttcat acatccccat taaacggtcg cagtactgcc
    16141 agtcgcggat cgatttttcc agcgttacgc gcttggcata cggaatattg taggaaatgc
    16201 cgccgccttc ataactggta aagccgctgg ccatcgagat ttcagccagc agacgcgcat
    16261 ctggcgtgcc gtggcggatc tgtagcggtt tttgcagagt ttcggtgagg cgacggcaag
    16321 ctgcgacgcc gtggttaacg accggtaaac cgttcagttt agaggttccg gcttcgatgg
    16381 attttttaat ccctacagca gcttcttcgt accgattcag acgggtatat gcatcgatgg
    16441 tgctcggcag cagatcgcac tcttcttgta gtgttttcag tagttcgatg tgttcgtcca
    16501 tcagcgccac gcctgcgcgc ggttggctaa gagttttacc ttccttgtct gctttcagca
    16561 gggcaagaga gaagcgttta tgctcaggaa tggtttgttg gtatttaacg ccatcttcaa
    16621 agttttcaac atccttgccg gtttcccagg ttttcaatac ctgttgccgc tcggtcatga
    16681 attcgttatg ggttaatttc ttatttcgaa gttccattga ttgcagcctt aattgttata
    16741 gggttaagca ttgaatactg gtgcgcgcgg cggccagtgg atcgactctg gcgacgtttg
    16801 ccagcagtgg taatagcccc cgggaatcgc ggtaatattc aaattcggtc ggtaagagaa
    16861 tgcgtttacc gtcgtcgttt aactcgcggt atttcaacca gtgatgcata tcgaactgac
    16921 tggcacgcga gagccatccg ccggaaccca cgactttacg cacggttgtc aggtcacgtc
    16981 ccatctgtaa atcgacattg ccgacgcagg tgcagacctc ttttttggtg cccgcatgtc
    17041 tttcggtggc ataacccaca cacagtccgg ccaggacggt atcgaaatat ttttcttctg
    17101 cattcgcggg taagtagtcg ggatgagcga ctagatgacg gaggtaatga taaaaagcgt
    17161 ccagctgtgc gggttgctga gcaaaattca cattgactaa ctctttcgca ctttcaccaa
    17221 ccaccattgc cgagacgcgc attcccagat cgccttccac cgtccgtttc acgaagggtt
    17281 ccggtacgcc atgtaaaaca gtgtcgggcg agagcgtgtt ggcacaggct gaatagacat
    17341 cggtggttgc gccgcccatg tcgatcagca tgaactcctt ccagctgtga tcgacgttac
    17401 tgattgcctt aaccagttca aagaccgtcc atggtgtcgg cattggttct tcgcctgttt
    17461 tatcaacaat gacatccagc cctttccctt taacgatgcg tttaaggaat atgtcgcaga
    17521 tagcctggcg tgcagctaaa gggttcggat ggtcaagatc gggcagaacg ttgtcgacga
    17581 tggtcaggtc tttatgccct aggatctcct gcacctcgtc ctgaatatcc cggtttccgg
    17641 cataaataat ggcgcagtcg agtttggatt ctgccagcac acgggcgtta ttcaggccgt
    17701 agctttcttc tccgccgtcg gtgccgccgg tgaacaagag aatatcgggc tgcgtttctt
    17761 ccagtgcctg gatatcacgg cggttcagct tgtaggcgta atattgcgca attttagccc
    17821 ccgcagagtg agcggttact ttcgcggttt ccagggtgat agaaggcacc aatcccattg
    17881 cggcaaccgc cagcccacct ttggcagagg aggaatactt caatgtaacc tcaccgctgt
    17941 taaacagggg gagggcatta tctacgttta acaccaggtt caggctggag aaaaacccct
    18001 tcgccagatg atgggtggtg gttggggtca gtacgtggtt aaccaacgtt aacgcgtccc
    18061 cctcccgggc gaagagggct gctttggtcc acgtcgaacc aatatcgaca gagacgattt
    18121 gcatcagatt gcctcttcaa gaatgcgagt atcaacgtta tgacgttggt tgatatcgtg
    18181 ggccatcagc tgacaaacat cttctaagtc atgacttggg gaaaagacgc gatcaaatcc
    18241 catctctttg aacttggttt ccacgtcagc gaagtcatgt tttccgacca ccaggttacc
    18301 gcccacatag agcaggatgt tacccagccc acgctcgatg cagcgttcgc gcatgcccag
    18361 acaatcgata tcgccatgcc catagataga ggagacgaca atcgcgtcag caccagtttc
    18421 aatggcagca tcaatatatt cgtcctggct gaccattacg cccagattaa taactctgaa
    18481 gtcatggttg ctaaaaacgc ggtccagaac tttattgcct actgcatggc agtccgcgcc
    18541 aataacgcca atcacaagtg ttgctttctt catgggtaat cctttgtaag acagagagta
    18601 aaataagtta cacacgtcgt atttatggcc gctcataacg gcacaggttt aatatcacag
    18661 gagtgtcttt atctgaccgt aatgttgcaa ttgatctatt ttcaaaatgt ctttcgggaa
    18721 atgattgttt aaagtgtgtt ttcaaattct cattttaatt cagtgtgtta tgtcaactaa
    18781 tgtcatgaat gttaaatttt taaaattaac ttgatttgat gtattgagat aattgtgatc
    18841 aacttctaat ttgcattttt gtgacgtggc tttagccatg agtaaatgca tttcataaaa
    18901 gttaagtcga atttcactgc aattcatctc tgcttaatgt aaaaataatg ttgaaaataa
    18961 caaataatta atgattcatg cgaattatga cctttgttag ctcttttgat ttttcgtaaa
    19021 aaagaaaagt acatgaaggt gctaccagta cgagtaacgt taatcaaaag ttttattacg
    19081 tctggcaata acgcaggcca ttttcgcgac tgggtggatt taatcagtat aatcctcata
    19141 caataggtca ggatttactg aaccgttaac ggggatttgg tgttgaaatt tagaaatggc
    19201 ggagtcaatt aatgaagaca atatcggaaa aatgacagtg tcagaattaa tttcaattaa
    19261 tccttctcgt tgttgaccaa aaaagatatt tatcttgatt tcatttgtac tacggcaatc
    19321 aatatgcagt aatttttgat cgctgagatt ttctgtttca taaatgtttt tggcgatagt
    19381 cggatgtatt gatgccagac atctgctcag ataagccaga tcctttaggg aacaccccgc
    19441 tgagtagagg atgtcagaga atttttgtgt gtcaactgtt gtctctgaag cgtatgttgc
    19501 attgacagga agggtgttga aagagatttg agatgggatc attatttaaa tctttccgtg
    19561 agtaataaaa attaatcccc gggcattgtt tggcaatatt tgccacaggg aatatataaa
    19621 aattaaagta tttgattggc atcggttaac tgatcaataa ccagtgccag attggtaaac
    19681 attttgaaaa agcgatactg gctcggaata tctctttcaa agacgaaaca ttcaatgtca
    19741 ttgtttgaaa aataaaggac tagtgttgct gattcgtcag gaaaaatatg gaaatatatc
    19801 tggttaccag aaggaggata gtccagaaga agggtatctt tgcccgtcac atacctgttg
    19861 tggtcgtgga tgctgcgctc acacttcatc agaggtgtca tgatactcat gtattcatct
    19921 gatgttaata tattaaaatc accatccatt ttaaagaaac gataaagtaa cagtacatcc
    19981 tcttgcgtaa ccggttcgtt tgtttttaga gggaaataag tgggaaccag aagatgaggt
    20041 tctaatctat tttgttgagc gctataacta aatttgaaca taaattccgg atcgctcatc
    20101 aattgagcga cataattatg cctggcttca aaaattttaa cttttatcag gttaaataag
    20161 tctgtttcgt catcctgtat taaaagacat tgaccatagc agccaggctt tttttcatta
    20221 taagcagaaa cggttattct gtcaggagtg tcaacgttat atccaaaagc cagatagtga
    20281 ccttcctcat cgagatttgt ttccaggtta tttaaattca tcacatgaag tgtttgcaat
    20341 gctgattcaa tgagctgttg actctctgag gtaaatgttt cgcgctgcgt tgtataaagt
    20401 gtatcgattc caacgactat ccgatgtgga tcaaggatgg cggcaatcat ctttattacc
    20461 tgcacagtca tgggtgatgt cattgattct ttatctgttt ttagaggatc attagatggc
    20521 caacctgaga agcgattgac agcagaactg atattatcag gactcatagc agcaacctca
    20581 ttgtaatcaa gcgaggcgct gagcgtactc ggctatagaa aaactaactt gcaaaaatcg
    20641 ctcatttttc atttatcagg cgaaagtggt ctctgttcac tcagaaacaa tcactcccgc
    20701 taagtcatac ctgtttcatc gatacattta agtgattatt actaatgaaa caataacata
    20761 acgggcttac ggccggtgct cataaaggca acaatcaatt aactttgata aaaatatgtt
    20821 atgtaaaata gcaacaaatt ggctaatcta atcaattggt attataaaaa gtggataggg
    20881 cggttctgtt aaataaaaca ctggtttttt atctatacct tttcattgta ataattgtgt
    20941 tgcataaata tttcacaata aattaacaat aaaatcacca atgatctatg gtttatacat
    21001 aattaacata tttggttggt tttgatataa atcaattttt aattcttgaa aaaaatctgc
    21061 aaaacacgat aaattgtcgt agatcaaatt ggtgagatcg tgacaatttc gctataaatt
    21121 gatcactgtc gaaaaatgca aatttgctca ataaaaacct gtttattgta aggattttgc
    21181 ggcgtaatat aattacggga tcaatttggt ttattcatta acatatttgt aactttttta
    21241 tcattgtctt ttcctctttt gatgaaatgg caacgataga ggcgagcaaa taattttgta
    21301 ttggggcatg cgtgtgaccc tttctaacgg ggttcactct cggagtcttc atgcgatgag
    21361 caaggagtca tgatgttaga tatagtcgaa ctgtcgcgct tacagtttgc cttgaccgcg
    21421 atgtaccact tcctttttgt gccactgacg ctcggtatgg cgttcctgct ggccattatg
    21481 gaaacggtct acgtcctttc cggcaaacag atttataaag atatgaccaa gttctggggc
    21541 aagttgtttg gtatcaactt cgctctgggt gtggctaccg gtctgactat ggagttccag
    21601 ttcgggacta actggtctta ctattcccac tatgtagggg atatcttcgg tgcgccgctg
    21661 gcaatcgaag gtctaatggc cttcttcctc gaatccacct ttgtaggtct gttcttcttc
    21721 ggttgggatc gtctgggtaa agttcagcat atgtgtgtca cttggctggt ggcgctcggt
    21781 tcaaacctgt ccgcactgtg gattctggtt gcgaacggct ggatgcaaaa cccaatcgcg
    21841 tccgatttca actttgaaac tatgcgtatg gagatggtga gcttctccga gctggtgctt
    21901 aacccggttg ctcaggtgaa attcgttcac actgtagcgt ctggttatgt gactggcgcg
    21961 atgttcatcc tcggtatcag cgcatggtat atgctgaaag gtcgtgactt cgccttcgct
    22021 aaacgctcct ttgctatcgc tgccagcttc ggtatggctg ccgttctgtc tgttattgtt
    22081 ctgggtgatg aatctggtta cgaaatgggc gacgtgcaga aaaccaaact ggctgctatt
    22141 gaagccgagt gggaaacgca acctgcgcct gctgccttta ctctgttcgg cattcctgat
    22201 caggaagagg agacgaacaa atttgcgatc cagatccctt acgcgctggg catcattgca
    22261 acgcgttccg tggatactcc agttatcggc ctgaaagaac tgatggtgca gcatgaagaa
    22321 cgcattcgta acgggatgaa ggcgtactct ctgctcgaac aactgcgttc tggttctacc
    22381 gaccaggcgg ttcgtgacca gttcaatagc atgaagaaag acctcggtta cggtctgctg
    22441 ctgaaacgct atacgccaaa cgtggctgat gcgactgaag cgcagattca acaggcaacc
    22501 aaagactcca ttccgcgtgt agcgccgctg tacttcgcgt tccgtatcat ggtggcgtgt
    22561 ggcttcctgc tgctggcaat catcgcgctc tctttctgga gtgtcatccg caaccgcatt
    22621 ggcgagaaaa aatggctgct gcgcgccgcg ctgtacggta ttccgctgcc gtggattgct
    22681 gtagaagcgg gctggttcgt ggctgaatat ggccgccaac cgtgggctat cggtgaagtg
    22741 ctgccgacag ctgtggcgaa ctcgtcactg accgcaggcg atctcatctt ctcaatggtg
    22801 ctgatttgcg gcctgtatac cctgttcctg gtggcagaat tgttcttaat gttcaagttt
    22861 gcacgcctcg gcccaagcag cctgaaaacc ggtcgctatc actttgagca gtcttccacg
    22921 actactcagc cggcacgcta agacaggagt cgtcaaatga tcgattatga agtattgcgt
    22981 tttatctggt ggctgctggt tggcgttctg ctgattggtt ttgcagtcac tgacggtttc
    23041 gacatggggg tgggcatgct cacccgtttc ctcggtcgta acgacaccga gcgtcgaatt
    23101 atgattaact ccattgcacc acactgggac ggtaaccagg tttggctgat caccgcgggc
    23161 ggcgcactct ttgctgcctg gccgatggtc tatgccgctg cgttctccgg attctatgtg
    23221 gcgatgatcc tcgtgctggc gtctttgttc ttccgtccgg tcggttttga ctaccgctcc
    23281 aagattgaag aaacccgctg gcgtaacatg tgggactggg gcatcttcat tggtagcttc
    23341 gttccgccgt tggtgattgg tgtagcgttc ggtaacctgt tgcagggcgt accgttcaac
    23401 gttgatgaat atctgcgtct gtactacacc ggtaacttct tccagctgct taacccgttc
    23461 ggcctgctgg ctggcgtggt gagcgtaggg atgatcatta ctcagggcgc gacttacctg
    23521 caaatgcgta ccgtgggcga actgcacctg cgtacccgtg caacggctca ggtggcggca
    23581 ctggtgacac tggtctgttt cgcactggct ggcgtatggg tgatgtacgg tatcgatggt
    23641 tatgtcgtga aatcgacaat ggaccattac gcagcctcta acccgctgaa taaagaagtg
    23701 gttcgtgaag ctggcgcatg gctggttaac ttcaacaaca cgccaattct gtgggctatt
    23761 ccggcactgg gtgtggttct gccgctgctg accatcctga ctgcacgtat ggataaagcc
    23821 gcgtgggcgt ttgtgttctc ctccctgacg ctggcctgca tcatcctgac tgccggtatc
    23881 gcaatgttcc cgtttgtgat gccgtccagc accatgatga acgcaagtct gacaatgtgg
    23941 gatgcaacat ccagccagct gacgcttaac gtcatgacct gggttgcggt ggttctggta
    24001 ccgatcattc tgctctacac cgcctggtgt tactggaaaa tgttcggtcg tatcaccaaa
    24061 gaagatattg aacgtaacac ccactctctg tactaagtaa ggagctaaaa atgtggtatt
    24121 tcgcatggat tctgggaacg cttcttgcct gttcgtttgg ggtaatcacc gcgctggcgc
    24181 ttgagcacgt cgaatcaggc aaagccggtc aagaagacat ctgatgagta agattatcgc
    24241 aaccttgtat gcggtaatgg acaagcgccc cctgcgggcg ctttccttcg tgatggcgct
    24301 tctgttagcc ggatgtatgt tttgggatcc atcacgtttc gccgcgaaga ccagtgaact
    24361 ggaaatctgg catggtttat tgctgatgtg ggccgtctgt gctggtgtga ttcacggcgt
    24421 gggctttcgt ccgcagaagg ttctttggca agggattttt tgcccattgc ttgccgatat
    24481 tgttctcatt gtcgggctga
//
LOCUS       DAPOZO010000072        24295 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPOZO010000072 DAPOZO010000000
VERSION     DAPOZO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.072"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..498)
                     /locus_tag="ROI21_003178"
     CDS             complement(<1..498)
                     /locus_tag="ROI21_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5112068.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGDRYLPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRLFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAA
                     GLYQVP"
     gene            complement(504..1202)
                     /locus_tag="ROI21_003179"
     CDS             complement(504..1202)
                     /locus_tag="ROI21_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDX5112069.1"
                     /translation="MQDIQQETLNECIRAEQSASVVLWEIDLTEVGGDRYFFCNEQNE
                     KGESVTWQSRQYQAYPIQGSGFEMNGKGSAARPTLTVSNLHGMITGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSEADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGSFLSINKLSQ"
     gene            complement(1202..1540)
                     /locus_tag="ROI21_003180"
     CDS             complement(1202..1540)
                     /locus_tag="ROI21_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5112070.1"
                     /translation="MANTEEFHWLPEDGMKTENKPSIKTVRFGDGYEQRSPNGLNHSL
                     RVFTCDFKVEANERDSFEKFLARHEGYKSFFWRPPGINRKIRVVCRTWSATEHITYTD
                     FSCQFDEVVI"
     gene            complement(1533..4766)
                     /locus_tag="ROI21_003181"
     CDS             complement(1533..4766)
                     /locus_tag="ROI21_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDX5112071.1"
                     /translation="MAEQTSRLAIIIDSTGAKNNADNLTSSLVKMTQAGETAANSAGK
                     VTKATEDEKNALAKLKAAIDPVGAAIDTVGRRYSELKKFFDKGIIDKEEYEFLVRKLN
                     ETTEELSGVAQAQREAEKAGKLAAAQQEAQTQAFQRMLDKIDPLAAALRNLEQQQDEL
                     NAAFASGKINGSQFENYSRKIQETRRELTGEAQAEREAAKAHDEQVAALQRLIAQLDP
                     VGTAFNRLVEQQKQLNEAKAKGMLSPEMYEELSGKLRAMRSELEVTQSQLSKTGMSAK
                     QTAFAMRMLPAQMTDIVVGLSTGQSPFMVLMQQGGQLKDMFGGIGPAIKGVGSYVLGL
                     INPFTLAAAAVGVLGLAYYKGSQEQDEFNKSLILTGNQLGTTSGQLADIVQRAGNAAD
                     STTGAAAAVLNQLVRSGKVASSSLEQVTTAIVKTSEVTGISTEQLVNDFNEIAKDPVS
                     AISKLNDQYHFLTIATYNQIKALQDEGNQQEAARIATEAYSSSMIQRTNQIKENLGYL
                     ETAWKAVADSAKWAWDSMLDIGREASLDQKISDVLRQIDEIEKNTRPGVFGLGGIGDG
                     GAQNKRLARLKQQLGVLQAEKIAQDVLNSSINDYNKRQQEGIELRQRADAFSKQYQTR
                     EQQRASELAKLEKLKSQYSKEEYNNLIAQINERYKGPKQPKAKGYSDDAAQRMIDHLN
                     QQNALLSSQTELTVKLSSSEQELVKWRQQIADLESRPSSKLTKDQKSLLLHREEITAL
                     MEKNVAIEKNNRLIKESAEIAAWRDSLQASIDNRQQGYDIQIAGYGVGDKNQQRQQEL
                     LRIEREYNNQRLQLERDYADKSRGMSNHVFQEKMQALNDALEREKEIVRQKNEQLDIQ
                     AGDWVSGASQGFNNWLDDTKDISEQIKSTTTQMFDGMTDALGDFVTTGKANFRSFATS
                     VISDLSRIALKASITGIFDSISNSSSGGILGTIGSAISKFIPNAKGGVYESPSLNTYS
                     NGIYDSPQFFAFARGAGVFGEAGPEAIMPLTRTSDGSLGVRAINSKSGNGGGDITYAP
                     VYQITIQNDGQNGEIGPQAIKALMGMVDQRVQGNLLNMRRDGGMLSG"
     gene            complement(4930..5130)
                     /locus_tag="ROI21_003182"
     CDS             complement(4930..5130)
                     /locus_tag="ROI21_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112072.1"
                     /translation="MLKELLYAYSVISRARRYAGMTGVPLPLSLTEINEYLATHPVLI
                     ERDEFEAVIFALDDQYFQEQCV"
     gene            complement(5239..5598)
                     /locus_tag="ROI21_003183"
     CDS             complement(5239..5598)
                     /locus_tag="ROI21_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112073.1"
                     /translation="MVASSDNDQYRSRNALIRRHIEKMDASLHVGTKEFDISKVSEVD
                     SVDDLLIDNAARYLLKDWKGVGELVNGAEVALEYTPERGIALLKQNPELYWQILAEAA
                     SIAQGKEQQKQDTIKKP"
     gene            complement(5749..6711)
                     /locus_tag="ROI21_003184"
     CDS             complement(5749..6711)
                     /locus_tag="ROI21_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112074.1"
                     /translation="MSSGAKVISAFIRETVAGTTPASGDWSLLKRTSWGVKPTQNKGE
                     NNEIGGSRMAQGATPGTVDVGGDVGTKFRWGQHDDFLASCFGAEWSGDSLTMGNERIT
                     FSLATYASDVGIASVVRGAQVGSWKMQIPNDGDITATVTFAGLGWESKADDTNFIKGE
                     PVDSAGKLRYSFKAVSAVSLNGVAGGNGFCIDSFDIQFDNKLQTQRCIGTGSPYAGAN
                     IPTTFTPSGTVTLSWSKAAWEIWSKTLTGETVPFSFTLSNGEGAYTFSFPKVQVSGEW
                     PDGGNTDIIQVQLSITAADEAPTITRKKCSPTAVIAKASADAIS"
     gene            complement(6738..7130)
                     /locus_tag="ROI21_003185"
     CDS             complement(6738..7130)
                     /locus_tag="ROI21_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HDX5112075.1"
                     /translation="MKFTEIRNTVISRMTAQTIIDGKDVLYPNGPTFDPSGKLIWARL
                     SNIPGQAGVNEIGAGPVVYRTGIIIIQLFVPAGSGSKLITETADKLRELFEFQDDDRL
                     SYQAVSSIAVGEKNGWFQLNLQIPYRAL"
     gene            complement(7127..7507)
                     /locus_tag="ROI21_003186"
     CDS             complement(7127..7507)
                     /locus_tag="ROI21_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDX5112076.1"
                     /translation="MAKAWDIEPSIFAGMIEEDVGLKIRYIAIQILTAIDIAAPVDTG
                     RFRNNNLVSLQHPDFGISDNVDPNGTIAVQRGIGVISKAANYGVIYIQNNLPYAEALE
                     NGHSQQAPTGVYANAFHGVLQAYK"
     gene            complement(7508..7891)
                     /locus_tag="ROI21_003187"
     CDS             complement(7508..7891)
                     /locus_tag="ROI21_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDX5112077.1"
                     /translation="MGLREEIQSEVAAAFDEDLADAVSDFSGSYVTHRHWDPVTETGG
                     ESTAIYTGRGVLTRYKLGRIDGINILHGDLKLTALVCEVTDKPAVDHIIEIYDPVSRQ
                     LQRYEVITASVDPSASVYSIQLRRA"
     gene            complement(7891..8286)
                     /locus_tag="ROI21_003188"
     CDS             complement(7891..8286)
                     /locus_tag="ROI21_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein singed"
                     /protein_id="HDX5112078.1"
                     /translation="MITYVTCDDVDNAFGNAWTSENAKNKAVLMANAWLNGFNLKINP
                     SRIPEEVKLAGAYAARIASFGKLFQQKNDSGVVISKAVSADGVSVSKSFSELPANSTA
                     LLEPDLQLAIALLKPYGLSRSQVRVVRGG"
     gene            complement(8290..8466)
                     /locus_tag="ROI21_003189"
     CDS             complement(8290..8466)
                     /locus_tag="ROI21_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004121546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112079.1"
                     /translation="MAGLTKEQRAQREAAQKTQIELVVMVTDYQMFPGAPTIANVHPD
                     EVDNWKAMGWKTQE"
     gene            complement(8509..9648)
                     /locus_tag="ROI21_003190"
     CDS             complement(8509..9648)
                     /locus_tag="ROI21_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 coat - protein 5 family protein"
                     /protein_id="HDX5112080.1"
                     /translation="MAGNTLTGLIPTIYTALDVVSREQVGFIPAVAKNAKADAAAKDQ
                     TVTAPVAPEAKTEDIVPGPSAPNTGDQNIGTVDVKITKSKMAPVKWNGEEQLALGPSG
                     TYNTILADQFKQAFRALANEVEADLGALYFGASRAVGTAGTTPFGVKDDLSDAALARQ
                     VLEDNGAPTTDLQMVLGSTAIANLRGKQSVLFKVNESGTEQLLREGVLGRLEGFNIHS
                     SAGVKRAPKVAATGYLVNGEKKEGDVLISIDTGSGCISAGQIVTFAGDPNQYVVAAAT
                     SNLITLAAPGLRQDLADDTAITVVGAFTANMAFDRNAFLLASRTPAMPEGGDNADDVM
                     NVTDPISGITFQIALYRQYRQVRYEVGLAWGVSSVKPAHGCLILG"
     gene            complement(9747..10511)
                     /locus_tag="ROI21_003191"
     CDS             complement(9747..10511)
                     /locus_tag="ROI21_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112081.1"
                     /translation="MKLKTVEINGKQYAEIDTAGLPVYVHDDGKEIGFDAPLATKKIT
                     ELNGEAKNHRLAKEAAEEKLAKFAAIEDPKKAIEALEMLSKIDQKKLLDAGQVDQVKA
                     EITKNFQQQLDEEKQRSQMLEKQLYDSMIGGSFAGSKYIADKIAIPADLLQARFGQAF
                     KVEEGRIVAYDASGNKIYSRAKPGELAQFDEALEFLVENYPQKDYILKASGNNGGGSR
                     PTQHDIGQKTMKRSAFDALDVAGKQNALKDGITIVD"
     gene            complement(10616..11728)
                     /locus_tag="ROI21_003192"
     CDS             complement(10616..11728)
                     /locus_tag="ROI21_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112082.1"
                     /translation="MQTVNERLRDESIAHAVWISRYSTGVAARMVKILNDSDAELTAR
                     LLIALDSLDPGSFTVKRLESLLASVREVNRTAINSMFTSLSGELNELAIYEAGYQLSL
                     FDSMLPDFVADVHPLVGISPDALYAAAMARPFQGRLLSEWASDLEADRLRRITNTVRQ
                     GFLLGDTNEQIARKIRGHVSKGFQDGALQMSRANAASIAKTAVGHLAATARESFASAN
                     NDLIKGKQWLSTLDNRTTPQCRIRDRLKYTLNNKPVGHSVPYLQGPGKIHFCCRSTET
                     FILKSAKELGIDVRDISPAERASMDGVVAGDTTYREWFLRQPYTRQKQIVGETRAKLI
                     RDGGMSPDEFYTDKGEWLTLKQLRERDAQVFRKAGI"
     gene            complement(11712..13118)
                     /locus_tag="ROI21_003193"
     CDS             complement(11712..13118)
                     /locus_tag="ROI21_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HDX5112083.1"
                     /translation="MADISTPNLDYNDMLEAWDINDALMGGTLEMRRQGENYLPKWPN
                     EDEDAYKKRLSVATLLPVYEESIKQNIGRIFAEPTVLSEETPAKIREYAENIDMEGSR
                     LDVWAQQFFSLAFQYGVAHALVDYPRTDMKEIRTKADENAAGGRPYVTMLNPRQVIGW
                     KSKVEKGKVVLTDLRIKEVIIIDGDDFGQKKVEQIRHIMPRRVEIYRRSEGTNGESVW
                     TLHESWNTSRDDIPLVTLYTKKTGFMRGTPPLLNLGLLNIKHWQSQSEQDNILHVARV
                     PLLVAYGLDRNEELTVGASTATIFEDRTKNGLEYVEHSGAAIESGETSLEKLENQMRH
                     AGAKLLRAENTSTKSVDQTNEERMQENSPLYTMANSLEDALDNILQIMAEWIGESCGG
                     NVDVRTELDVSAQVFDSSSALAVQSLRQGGDIRQIDAVRVLQALKFIDQDSRPEEVID
                     ELKNQSVMLMEINDANRE"
     gene            complement(13121..14422)
                     /locus_tag="ROI21_003194"
     CDS             complement(13121..14422)
                     /locus_tag="ROI21_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDX5112084.1"
                     /translation="MISPTLNVPQARFLSMPHKFKAYIAGFGSGKTWVGCGGICKGIW
                     EHPGINQGYFAPTYPQIRDIFYPTVEEVAADWGLNVKINEGNKEVHFYYGRQYRGTTI
                     CRSMEKPQTIVGFKIGNALVDELDILPKEKARTAWRKIIARMRYKIDGLRNGIDVTTT
                     PEGFKFVYEQFVKAVREKTELASLYGLVQASTFDNEKNLPADYIPSLLESYPPELIKA
                     YLRGQFTNLTSGTVYHQFDRKLNNCEEVEQPGEPIYIGMDFNVGKMAGIVHVLRLGLP
                     CAVTEIINAYDTPDMIRIIKERFWLYDGNDYRKVREIYIYPDASGDSRKSSNASTTDI
                     AQLKQAGFNVVVNSSNPPVKDRVNSMNAMFCNANGERRYKVNVKRCPLYAESLEQQVW
                     DEKGEPDKKSGNDHPNDAGGYFIVKQFPIVKPTGRVTSLRI"
     gene            complement(14403..15497)
                     /locus_tag="ROI21_003195"
     CDS             complement(14403..15497)
                     /locus_tag="ROI21_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDX5112085.1"
                     /translation="MSRPDWGALQQEYIAEYTRSGISPVAWCEARGLNYATARRYIKK
                     TPKNAQTEMCKTAQKSAQKKSAQTAQKRNEKSQEKKPVFDAGLNEGDAEEFSFCPDEF
                     GISDQQAKFAMLVAQGKKPTEAYRLAGYEGQGATANSNASRMLRNARVFRAISYFRNQ
                     YQKRYTADLDLLVSQLMAIVLADPNQLAQFRRVNCRYCWGENHLYQWRDIAEFDKAAA
                     QASRDGKPEPEYGGLGFVDNAIPNPDCPKCCGEGTGQLYMADTTLLDGDARQLYAGAK
                     FGKFGVEILLEDKAAARRELLRLLSAGGALCADKRLQELEIERRRMENQKLRKEIETV
                     EDNEHPQPVAININVVDARVRSDEDDLSDA"
     gene            complement(15501..15752)
                     /locus_tag="ROI21_003196"
     CDS             complement(15501..15752)
                     /locus_tag="ROI21_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112086.1"
                     /translation="MIPYSKVESLAACRMTAQQIADVLDVDLNRLKENREAMTDFYAA
                     IRKGRAKGEAELRAALFKLARKGDAFALRELLRVDKNQD"
     gene            complement(15688..16620)
                     /locus_tag="ROI21_003197"
     CDS             complement(15688..16620)
                     /locus_tag="ROI21_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112087.1"
                     /translation="MRDDFCAFILTHGRPDKVLTYRTLRRAGYTGKIFIVVDDEDKTR
                     HQYMAEFGEQVLVFSKADIASRFDEADNFCDRRSIFYARNACFDLAKLVGCKYFIQLD
                     DDYHEFQFRVDRNYDQAYFPIRKLDAILSEMLAYYESIPALSIAMSQGGDFLGDNGGH
                     ASWVKRKAMNSFICSVERPFSFMGRINEDVNTYTNLGRCGELFMTIGAVQLGQKQTQK
                     NSGGMTELYLDSGTYVKSFYSVMYAPSCVKISLMGASHKRIHHQVTWNNAAVKILHEK
                     YRKKTPCISMGVTNDSVFESRVSGSVPDDCTTNR"
     gene            complement(16613..17404)
                     /locus_tag="ROI21_003198"
     CDS             complement(16613..17404)
                     /locus_tag="ROI21_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDX5112088.1"
                     /translation="MSEKLKIVYRPLQELSPYAHNARTHSTEQVAQLVESIKQFGWTN
                     PVLIDEKGEIIAGHGRVMAAEMLKMDSVPVIVLSGLTDEQKKAYRLADNRLPMNAGWD
                     EDLLRMELSDLINADFDVSLTGFIPTEIDELLTDVLPGTGNEEEPYTTKIDTPVYEPS
                     GGKPDISELYDDTKTQELISRIRSASLEPDIEKFLLCAAERHTVFNFSRIADYYAHAP
                     SEIQCFFEESALVIIDYQQAIENGFVRMTQRMVEIMHGGEEEEYA"
     gene            complement(17542..18999)
                     /gene="trkG"
                     /locus_tag="ROI21_003199"
     CDS             complement(17542..18999)
                     /gene="trkG"
                     /locus_tag="ROI21_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkG"
                     /protein_id="HDX5112089.1"
                     /translation="MNTSHVRVVTHMCGFLVWLYSLSMLPPMVVALFYKEKSLFVFFI
                     TFVIFFCIGGGAWYTTKKSGIQLRTRDGFIIIVMFWILFSVISAFPLWIDSELNLTFI
                     DALFEGVSGITTTGATVIDDVSSLPRAYLYYRSQLNFIGGLGVIVLAVAVLPLLGIGG
                     AKLYQSEMPGPFKDDKLTPRLADTSRTLWITYSLLGIACIVCYRLAGMPLFDAICHGI
                     STVSLGGFSTHSESIGYFNNYLVELVAGSFSLLSAFNFTLWYIVISRKTIKPLIRDIE
                     LRFFLLIALGVIIVTSFQVWHIGMYDLHGSFIHSFFLASSMLTDNGLATQDYASWPTH
                     TIVFLLLSSFFGGCIGSTCGGIKSLRFLILFKQSKHEINQLSHPRALLSVNVGGKIVT
                     DRVMRSVWSFFFLYTLFTVFFILVLNGMGYDFLTSFATVAACINNMGLGFGATASSFG
                     VLNDIAKCLMCIAMILGRLEIYPVIILFSGFFWRS"
     gene            complement(19196..19381)
                     /gene="rzoD"
                     /locus_tag="ROI21_003200"
     CDS             complement(19196..19381)
                     /gene="rzoD"
                     /locus_tag="ROI21_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HDX5112090.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSGNDW"
     gene            complement(19598..20074)
                     /locus_tag="ROI21_003201"
     CDS             complement(19598..20074)
                     /locus_tag="ROI21_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HDX5112091.1"
                     /translation="MQTLNSQRKAFLDMVAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGDIRQTIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(20078..20419)
                     /locus_tag="ROI21_003202"
     CDS             complement(20078..20419)
                     /locus_tag="ROI21_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDX5112092.1"
                     /translation="MKMHNAPHSWPDLLELLQSWWRGDTPLGAVVMSIIMAGLRIAYF
                     GGGGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMR
                     VIGNKFGAHKE"
     gene            20496..20798
                     /locus_tag="ROI21_003203"
     CDS             20496..20798
                     /locus_tag="ROI21_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112093.1"
                     /translation="MFPEPNNEKAPGHAGARWSARLSFWRYILVFDIVKSPKVTIQTK
                     RIFMSEKNKPQGENKPQQPVAPKPTPTQSTTDFATRRVFVGDSADSVIEHIKKQPR"
     gene            complement(20767..21336)
                     /locus_tag="ROI21_003204"
     CDS             complement(20767..21336)
                     /locus_tag="ROI21_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112094.1"
                     /translation="MRELPKDYFLGVDDELVDYLEKQGEETIREIHLSNKTNVENGYK
                     LLNIQIVGIGSSFLLLTQKTNFDFLTAGITTFTLLWTWCAIYLVCTGLSVKVRGLINA
                     PPDHLYHEKYKDMEPSSFKIFADAGYLGPDKLLPLIRRYRLVDLSDTARELLLENEKI
                     RTSLDKARMYTILAPVAAMFISAVFLYVQ"
     gene            complement(21558..22100)
                     /locus_tag="ROI21_003205"
     CDS             complement(21558..22100)
                     /locus_tag="ROI21_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HDX5112095.1"
                     /translation="MIYPTNTGKSGEHLRLATLESVWIQGKLRMWGRWSYIGGGRSGN
                     MFNQLLASKKLTKTAINEALRRMKKAGIEKAELEAFLREMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKIA"
     gene            complement(22097..22387)
                     /locus_tag="ROI21_003206"
     CDS             complement(22097..22387)
                     /locus_tag="ROI21_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDX5112096.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(22387..22986)
                     /locus_tag="ROI21_003207"
     CDS             complement(22387..22986)
                     /locus_tag="ROI21_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDX5112097.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHLYPDGSHGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVTAQLLEFA"
     gene            23854..24195
                     /locus_tag="ROI21_003208"
     CDS             23854..24195
                     /locus_tag="ROI21_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDX5112098.1"
                     /translation="MTHKRIPKDWVIKRSTPFFTKENVPSALLTHHNTAAGVFGQLCV
                     MEGTVTYYGFADENATEPEIKVVINAGAFATSPPQYWHRVELSDDAQFNINFWVAPDF
                     SGEKVYTAKKE"
BASE COUNT         6397 a         6359 c         4936 g         6603 t
ORIGIN      
        1 cggcacctga tacagccctg cggcctccag attatccaga tagagattct gaccgttacg
       61 ccaccagtca tcctcacgat gaaaatccgg catctcaatc cccgccagat ggtaagcatc
      121 ccggaacagc gtgtaacagt ccgtcacccc gtgctcaaag agccgcccgg tgagatgcgg
      181 cacacagcgg aatttatgaa tcgccccccg gcagaccagc caccacggca aatcactctg
      241 cacctgcaac cgccggtcgg cctcactcag ccagggcaga ccaccgggat ggctgtgcac
      301 cagcgccaca atctcaccct gcatttctgc ccgcagccag tcctccggcg acatccggaa
      361 atacgcctcc ggctcaccgg agatatttac gcagggaaga tatctgtctc cctccggcgt
      421 tctcaccaca aagccgcacg actccgctgg cgcacatcgc cgggcgtgcg ccagaatcgc
      481 tgattctgtc tctgtcatgg gatttactgc gaaagtttgt taatggaaag gaagctgcca
      541 aagttgccga cattattgcg aaacttacag ccactcaggc atttgctgca tttatccttc
      601 gtgatatcgg acgtcggctg gtcatattca tccgcgaccg ccggaccgct ataaccgcac
      661 tcatcgccgc gataggtcca ggtgcaggtg ttggccagca tgatacgtcc cggaaaaaca
      721 gcgccatccg tttcagtcgg cgtggacagt acaaaagagg cactgaccgc gctcagttcg
      781 ctgcactgct cgatgcgcca gcggctgatc acctcctgct ccggatcggc ttcgctgttt
      841 ccgttgacga agttcaccgc atccagaaag cgggcgtaaa ccttacgccg gaccaccgtt
      901 ccgccgacca gactctgcag atcttccgcc atcccggtga tcatgccgtg caggttagag
      961 accgtcagtg tcggacgggc agcactgccc ttgccgttca tctcaaatcc actcccctga
     1021 atgggatagg cctgatactg ccgactctgc caggtgacag actcaccttt ttcgttctgc
     1081 tcattacaga aaaagtagcg atccccgcct acctcggtca ggtcgatttc ccagagcacc
     1141 acgctggccg actgctccgc acgaatgcat tcattcagcg tttcctgttg tatgtcctgc
     1201 atcagatcac cacttcgtca aactgacacg aaaaatcggt ataggtgata tgttctgtcg
     1261 ctgaccacgt tcgacacacc actctgattt ttctgttaat acccggcggg cgccaaaaaa
     1321 aagatttata gccttcatgc cgggctaaaa atttttcaaa tgaatcacgt tcattcgcct
     1381 caactttgaa atcacaggtg aaaacgcgca gagaatgatt aagtccattt ggacttcgtt
     1441 gctcataacc atcgccaaat cttacagttt ttatcgatgg tttattttct gttttcattc
     1501 catcctctgg taaccagtga aattcttccg tattagccac ttaacattcc cccatcacgt
     1561 cgcatattta acagattgcc ctgcacccgc tgatcaacca tccccataag tgcttttatt
     1621 gcctgagggc caatctctcc attctggccg tcattttgaa tagtgatttg gtatacaggg
     1681 gcataggtaa tatctccgcc tccattacca cttttactat taatagctct gacaccaaga
     1741 gaaccatcgg aagttcgtgt taatggcata atggcttccg gaccagcctc accaaaaaca
     1801 ccagcccctc ttgcaaaagc aaaaaattgc ggggaatcat aaataccgtt cgaatacgtg
     1861 ttcaatgacg gagactcata aacaccgccc tttgcattcg gaataaattt actaatagca
     1921 ctcccgatag ttcctaaaat ccccccagaa gaactgttac taatgctgtc gaaaatccca
     1981 gtaattgaag cctttaatgc tattcgacta agatccgaaa tcacggaagt agcgaaagaa
     2041 cgaaaatttg ctttgcctgt cgtgacaaaa tcacccagcg catcggtcat tccatcaaac
     2101 atctgagtcg tggttgattt tatctgctca ctgatatcct tagtgtcatc cagccagtta
     2161 ttgaatccct gggaggcacc actaacccag tctcctgcct gaatatcgag ctgctcgttt
     2221 ttctgtctga caatttcttt ttctcgttcc agagcatcat tcagagcctg cattttctcc
     2281 tgaaaaacat gatttgacat cccacgggat ttatctgcat agtcacgttc aagttgcaga
     2341 cgctgattgt tatattcacg ttcaatccgc agtaattcct gctggcgttg ctgattttta
     2401 tcgccaaccc cataaccagc aatctgaata tcatacccct gttgacgatt atcaatcgaa
     2461 gcctgcaatg aatcacgcca tgcagctatt tcggcggatt ccttgattag cctgttattt
     2521 ttttcaatcg caacattttt ctccatcaac gcggttattt cttcccggtg taagagaagc
     2581 gatttctgat ccttggttaa tttcgatgac ggtcgtgact ccaggtcggc aatctgctga
     2641 cgccacttaa ccagttcctg ttcagaggaa cttaatttaa cggttaattc agtttgtgaa
     2701 cttagtaacg cattctgttg attcagatga tcaatcattc gttgggcagc atcatccgaa
     2761 taacctttcg cctttggttg ctttggacct ttataacgct catttatttg agcgataaga
     2821 ttattatatt cttcctttga atactgactc tttagttttt ccagttttgc aagttcacta
     2881 gctctctgct gctcccgggt ctgatattgt tttgaaaagg catctgctct ctgtctgagt
     2941 tcaattcctt cctgttgtcg cttgttgtaa tcgtttattg atgaatttag tacgtcctga
     3001 gcaatttttt ctgcttgaag tacgcccaat tgctgcttta atcgtgccag ccttttattt
     3061 tgagctccgc catctccaat gccacctaac ccgaaaactc cgggtcgggt atttttttct
     3121 atttcatcaa tttgacggag aacatctgag attttttgat caagggaggc ctcacggcca
     3181 atatccagca tggaatccca tgcccatttt gcggagtctg cgacagcttt ccatgcagtc
     3241 tcaagataac caagattttc tttaatctgg ttggtgcgct ggatcattga ggatgagtat
     3301 gcttctgtcg caatgcgggc ggcctcctgc tggttccctt catcctgtag cgccttaatc
     3361 tggttataag tcgcaattgt cagaaaatgg tactgatcat taagttttga tatggcactg
     3421 acaggatcct ttgcaatttc attgaagtca ttaaccagtt gttcggttga tattcctgtt
     3481 acttcgctcg tttttactat cgctgtcgtc acttgctcca gcgaactgct cgctaccttt
     3541 cccgaacgca caagctggtt taatactgcc gccgcagcgc cagttgtcga atcagccgca
     3601 ttcccggcac gttgaactat atcggccaat tgcccgctgg ttgtccccaa ctgatttccg
     3661 gtaagaataa gagacttatt aaattcgtcc tgctcctgag agcctttata gtaagccagc
     3721 cctaagacgc caacggctgc tgcggccagg gtaaaaggat taattaatcc cagcacataa
     3781 gaaccaacac ctttgatcgc cgggccaatc ccaccgaaca tatcttttag ctggccgccc
     3841 tgctgcatta acaccataaa aggcgactga ccagtggaca acccaacaac aatatccgtc
     3901 atttgtgcag gcaacatgcg catagcaaaa gccgtttgtt ttgccgacat tccggttttg
     3961 cttaattgag attgagtaac ctcaagctca ctccgcatag cacgaagttt tccagaaagt
     4021 tcctcataca tttcaggaga gagcatcccc ttagcttttg cttcattgag ctgtttctgt
     4081 tgttctacca gacgattaaa agcagttccg acagggtcaa gttgagcaat cagacgttgc
     4141 aaagcagcaa cctgttcatc atgcgctttt gctgcttctc gctctgcctg agcctctccg
     4201 gtaagctctc gccgtgtttc ctgtattttt cggctataat tctcaaactg agaaccattt
     4261 attttcccgg atgcaaacgc agcattaagt tcatcctgct gttgttcaag atttcttagc
     4321 gccgcagcca gagggtcgat cttgtccagc attctttgaa aggcctgagt ctgcgcttcc
     4381 tgctgggcgg cagcaagttt tccggccttc tcggcttctc tctgcgcttg cgcaaccccg
     4441 ctcaattcct ctgtggtttc attaagttta cggacaagaa attcatattc ttctttatca
     4501 ataatccctt tatcgaaaaa tttctttaat tcagaatagc gtcgaccgac agtatcaatt
     4561 gcggcaccaa ctggatcaat agctgctttt aattttgcga gcgcgttctt ctcatcttct
     4621 gttgccttag tcactttccc tgcgctattt gcagcagttt ccccggcctg cgtcattttg
     4681 actaatgagg aggtcagatt gtcagcatta ttttttgctc cagtgctatc aataattatt
     4741 gcgagacgcg aggtttgctc tgccatttat taaaactcca gacaacaaaa agcccaccga
     4801 agtgggtttc aggcgacata atagtagata tagcgattac gaggccacgc aatgcttttc
     4861 tccaggagca tcatcgattt aattaaagac accatcacat ctctgtaaca gagtgtacgt
     4921 aattaacaac tacacacact gctcctgaaa atactggtca tccagtgcaa agatcactgc
     4981 ttcaaactca tcgcgctcaa tcaataccgg atgagtggct aaatattcat ttatctctgt
     5041 cagagataaa ggcaaaggca ccccggtcat tccagcataa cgtcgggcac gggatattac
     5101 cgaataggcg tacaacaatt ccttaagcac cgggtctatt tctggttccg gtatcggtgg
     5161 caacctgagt ttttctcgct tccatcttgc cttttccccc cctttctccc ccgaactccg
     5221 ataaccaccg ctgggcggct atggcttttt tatcgtatcc tgcttctgct gctctttacc
     5281 ctgggcgatg ctggctgctt ctgcaaggat ctgccagtac aactctggat tctgcttaag
     5341 cagcgcgatc cctcgttctg gcgtatattc cagtgcaacc tcagcaccat taaccagttc
     5401 accaacccct ttccagtctt tcagcagata acgagcagca ttatcaatga gtaaatcatc
     5461 aacagaatct acctcggaaa cctttgaaat atcaaactcc ttcgttccga cgtgcaaact
     5521 ggcatccatt ttctcaatgt gacgacggat taatgcatta cgggagcgat actgatcgtt
     5581 atcgctgctt gccaccaaca gttttaaccc gtctataggt tttaagtcct tcattggcgt
     5641 aaaccagcgt tctccaccga tgataatttt ctgattaaga ataaacatcc ataacctcat
     5701 tcaatgcgtc cccctgtagt gcagtaccac agggggaata acggaaaatc aactaatcgc
     5761 atcagcactg gcttttgcga tcacggcagt cggggagcat ttttttctgg ttatcgtagg
     5821 tgcttcatct gctgcggtaa tgctcaattg aacctggata atgtcagtat tacccccatc
     5881 aggccattca cctgacacct gaaccttcgg gaaactgaaa gtgtatgccc cctctccatt
     5941 cgaaagcgtg aagctgaacg gaactgtttc tccagtcagt gttttactcc agatttccca
     6001 cgcggcttta gaccatgaaa gcgtcaccgt accggacggt gtaaaagtag tcggaatatt
     6061 tgctcctgca taaggcgagc cggtcccgat acaacgctgt gtctggagtt tgttatcgaa
     6121 ctgaatatca aaactgtcga tacaaaaacc gttacctccg gcaacaccat tcaggcttac
     6181 tgctgaaacc gccttaaacg aatagcgtaa ctttcctgca ctatccacag gttcgccttt
     6241 gataaaattc gtatcatcgg cctttgattc ccagcccagc ccggcaaagg ttacggtcgc
     6301 tgtaatatcg ccgtcgttag ggatctgcat tttccatgag ccaacctgcg ctcctctgac
     6361 gacagaggca attccgacat cggacgcata ggtcgccaga gaaaatgtta ttcgctcatt
     6421 ccccattgtc agagaatcgc ctgaccattc cgcgccgaaa caggatgcaa gaaaatcatc
     6481 atgttgaccc cagcgaaatt tggtaccaac atcaccgccg acatccacag tgccaggcgt
     6541 cgccccctga gccatccggg agccaccaat ctcattattt tcgcctttat tctgggtggg
     6601 ttttactccc cagcttgtgc gttttaataa actccagtca ccacttgctg gcgtagtgcc
     6661 tgcaactgtc tcccggataa atgccgagat aacctttgca cctgaactca caggagcctc
     6721 ctatgtcatt aattgcgcta gagcgcgcga tatggaattt gaagattaag ctggaaccag
     6781 ccattctttt cgccgacggc tattgaggaa acagcctggt aactgagacg atcgtcatcc
     6841 tgaaactcaa acagttcccg caatttatcg gctgtctcag taatgagttt tgaaccagaa
     6901 cctgcgggga caaataactg aataatgatt atccccgtgc gataaacaac cggccccgcg
     6961 ccaatttcat taactccagc ttgcccggga atattactta accgcgccca gattaactta
     7021 ccggaaggat cgaacgttgg cccgttcgga tacaatacgt cttttccatc aataatcgtc
     7081 tgtgccgtca ttctggaaat aaccgtattt ctgatttcag taaacttcat ttgtaagcct
     7141 gtaaaacacc atgaaaagcg ttggcataca cgccagttgg cgcttgttgt gaatgaccgt
     7201 tttcaagagc ctctgcataa ggaaggttat tctggatata aataaccccg taatttgcag
     7261 ctttcgaaat aaccccgatc ccacgctgaa cagcaatcgt accgttcgga tccacgttat
     7321 cagatatacc aaaatcggga tgctgtaacg acaccaggtt gttatttctg aaacgtccgg
     7381 tatcaaccgg agcagcaata tcaatagcag taagaatctg aatagcgatg tagcgaatct
     7441 tcagccctac atcttcctca atcatcccgg caaatattga cggttctata tcccatgcct
     7501 ttgccattta cgctctcctt aactggatag agtaaactga tgcggaagga tctacacttg
     7561 ctgtaattac ctcgtatcgt tgtaactgcc ttgatacagg atcataaatc tcaataatat
     7621 ggtcgacagc tggtttatcc gtaacctcgc ataccagagc ggttaattta aggtcaccat
     7681 gcaaaatatt aatcccatca attctgccca gcttataacg tgtcaacacg cctcgcccgg
     7741 tatagattgc ggtggattcg ccgccagttt ccgtcacagg atcccagtgc cggtgcgtaa
     7801 cgtaagaacc agaaaaatca ctcacggcgt ccgctaaatc ctcatcaaaa gcagcggcaa
     7861 cctctgactg aatctcttca cgaagcccca ttatcccccc ctcacaaccc tgacttgtga
     7921 gcgactaagt ccgtacggtt tcagcagtgc tatcgcaagc tgtaaatcgg gttcaagcaa
     7981 tgcagtgctg tttgctggca actcagaaaa tgatttcgat acactgaccc cgtcagccga
     8041 cacggcttta ctgataacaa cgccagaatc atttttctgc tgaaacaact taccgaatga
     8101 ggcaattctg gctgcatatg ctcccgcaag ttttacctct tccggaatac gggatgggtt
     8161 aattttcagg ttgaagccat taagccaggc attagccatt aaaacagctt tatttttagc
     8221 gttctcactc gtccaggcgt tcccaaacgc attatcaacg tcatcacagg tcacgtaagt
     8281 gatcatgtgt tactcctgag ttttccagcc catagccttc cagttgtcaa cttcatcagg
     8341 atgaacatta gcgatagttg gtgcgccggg aaacatctga taatcggtca ccataaccac
     8401 taactcaatt tgcgtttttt gtgcggcttc acgctgtgct ctttgctctt tagttaatcc
     8461 ggccatatgc cccccattaa aaatggggcc gaagccccgt ttgaatgttt aaccaagaat
     8521 cagacaacca tgtgccggct tcactgatga aacaccccat gccagtccaa cttcatagcg
     8581 cacctggcga tactgacgat acagtgcaat ctggaacgta atccctgaaa tcgggtccgt
     8641 aacattcatt acatcatcag cattatcgcc accttccggc attgccggag tacgggatgc
     8701 cagcagaaac gcgttgcgat caaacgccat atttgcagta aaggccccaa caactgtgat
     8761 ggcagtatca tcggccagat cctgacgcag tccgggcgct gcaagagtaa tcagattgct
     8821 ggtcgctgct gccacaacat actgattcgg atcaccagcg aacgtaacaa tctgacctgc
     8881 agaaatacac cccgaaccag tatcaatgga aataagaaca tcgccttctt ttttctctcc
     8941 attcacgaga taaccagttg cagcaacctt tggcgctcgt ttcacacctg ccgaactgtg
     9001 aatattgaat ccctccagac gccccaacac gccctcacgc agtagctgtt cagtgccgga
     9061 ttcattcact ttaaacaata cagactgttt tccgcgcaaa ttagcaatgg cagtggagcc
     9121 aagcaccatc tgcagatcgg ttgtcggtgc gccgttatcc tccagaacct gacgagccag
     9181 agcagcatca gaaagatcat ctttaacacc aaacggcgtt gtccctgcgg ttcccacggc
     9241 gcgggaagca ccgaaataca acgcaccaag atcagcctca acctcgtttg caagggcgcg
     9301 aaaagcctgc ttgaactgat cagccagaat ggtgttgtaa gtccctgaag ggccaagagc
     9361 cagttgttct tcaccattcc atttaaccgg tgccattttg gatttagtaa tttttacatc
     9421 aacagtgcca atattttgat caccggtatt cggagctgac ggccccggta cgatatcttc
     9481 ggttttcgcc tcaggcgcaa ctggcgcggt taccgtctga tcttttgctg cggcgtcagc
     9541 ttttgcgttt tttgctaccg cagggataaa acctacctgc tcacgggata caacatccag
     9601 ggcggtataa atagtcggga tcaacccggt cagggtattt ccagccataa ttaaatattc
     9661 cttaaaaatt tgcgtaattg tgaatagatg gagtaatgag ctatccagcc ctgacaccag
     9721 ctcccatccg gaagctgaca aatgtgttaa tcaacgattg tgataccgtc tttcaatgcg
     9781 ttttgcttac ctgcaacatc cagtgcatca aaagcagagc gtttcatcgt tttctgacca
     9841 atatcatgct gtgtcggacg ggagccgccg ccattgttgc cactggcttt caggatgtag
     9901 tctttctgag ggtaattttc gacaaggaac tccagcgcct catcaaactg cgccagttcg
     9961 ccaggcttcg cgcgggaata aattttgttg ccggaagcgt cataagcaac gatcctccct
    10021 tcttccactt tgaatgcctg cccgaagcgg gcttgtaata aatctgccgg gatcgcaatt
    10081 ttatcggcaa tatattttga acccgcaaaa ctaccgccaa tcatagaatc gtaaagctgc
    10141 ttctccagca tctgagagcg ttgcttttct tcatctaatt gctgctgaaa atttttcgta
    10201 atttctgcct taacctggtc aacctgtccc gcatcgagca gctttttctg gtcgattttt
    10261 gacagcattt ccagtgcctc gatcgccttc ttcgggtctt cgatagcggc aaacttagcc
    10321 agtttttcct ctgcagcttc tttagccagg cgatgatttt ttgcctcgcc attaagctct
    10381 gtaatttttt ttgtcgccag cggtgcatcg aagccaattt ctttaccatc gtcgtgcaca
    10441 taaactggca ggccagcagt atcaatttct gcgtattgtt ttccgttaat ctcgaccgtt
    10501 ttcagtttca tattagtacc tggtttaagt cttccgacag ttacgctgct caccatccgg
    10561 atcgcagcaa taaaaaaggc cacccgaagg tagcctgttg taataaatga tttatttaaa
    10621 tccctgcttt tctgaatacc tgtgcatcac gctcacggag ctgcttcagc gtcagccatt
    10681 cgcctttatc ggtgtaaaat tcatctggcg acataccacc atcccgaatc agctttgctc
    10741 gggtttcccc cacaatctgt ttttgtctgg tgtaaggctg acgcaaaaac cattccctgt
    10801 aggttgtatc tccggccacc acgccatcca tgctggcccg ctcagccggg gaaatatcgc
    10861 gaacatcaat acccagttcc ttcgctgatt tcagaatgaa cgtttccgtt gagcggcagc
    10921 agaaatgaat ttttcccggc ccctgcaaat aaggcacgct gtgacctaca ggtttattat
    10981 tcagcgtata tttgaggcga tccctgattc gacattgtgg cgtagtacga ttatcaagcg
    11041 ttgataacca ttgcttaccc ttaatcaaat cattattcgc gctggcaaaa ctctcacggg
    11101 cagtagcagc aagatgtcca accgctgttt ttgcaatgct ggccgcatta gcccggctca
    11161 tctgcaatgc accatcctga aatcccttac tgacatgtcc ccgaattttt cttgcgatct
    11221 gctcattggt atcccccagc aaaaaaccct gacgcactgt atttgttatg cgtctgagcc
    11281 gatccgcttc aagatctgag gcccactcac tgagcagtcg tccctggaat ggtcgcgcca
    11341 ttgcagcggc ataaagtgca tcaggagaga taccaaccag agggtgaaca tcagcaacaa
    11401 aatcaggtag catagaatca aacagactta actgataacc agcctcataa attgccagct
    11461 cgttcagctc tccggagaga ctggtaaaca tgctgttaat agcagtgcgg ttaacctctc
    11521 tgacgctcgc cagaagtgac tccaggcgct taacggtaaa actacctgga tcgaggctgt
    11581 ccagtgctat cagcaggcga gcagtaagtt ccgcatcgct gtcattcagt attttcacca
    11641 ttctggcagc cacgccagtg ctatagcggg atatccagac tgcatgagca attgattcat
    11701 cacgcagccg ttcattcacg gtttgcatca ttgatttcca tcagcattac actctgattt
    11761 tttaattcat cgatcacttc ctctggacgg gaatcctgat cgataaattt caacgcctgc
    11821 aacacccgaa ccgcatcaat ctgacgtata tcaccgccct gacgcaatga ctgaacagcc
    11881 agcgcggagg atgagtcaaa cacctgggca gatacatcca gttcagtgcg cacatccaca
    11941 ttgccaccgc aactctctcc gatccattcc gccattatct ggagaatatt atcgagggca
    12001 tcttcgaggg agttcgccat tgtataaagc ggcgagtttt cctgcattcg ctcttcatta
    12061 gtctgatcaa cagatttggt ggatgtattt tcagcacgca gaagtttagc gccggcatga
    12121 cgcatctgat tttccagctt ctcaagtgat gtttcgccag attctatcgc tgcgccacta
    12181 tgttcaacat attcgaggcc attttttgtt ctgtcctcaa aaatcgtagc ggtggatgca
    12241 ccaaccgtca gttcttcatt cctgtccagc ccgtaggcca ccagcaatgg aacgcgggca
    12301 acatgaagaa tattgtcctg ctcgctctgg ctttgccagt gcttgatatt cagcaagcca
    12361 agattaagca atggcggtgt accacgcata aatcctgttt tcttcgtgta cagtgttacc
    12421 agaggaatat catcacggct ggtattccat gactcatgaa gcgtccagac agattcgcca
    12481 ttagtgcctt cgctacgtcg ataaatttca actcgacggg gcataatatg gcggatctgc
    12541 tccaccttct tctgcccgaa atcatcacca tcaataatga tgacctcttt tatacgcaaa
    12601 tcagtgagaa caactttccc tttttcaact ttcgatttcc atccaataac ctggcgtgga
    12661 ttcagcatcg taacgtacgg acgaccaccg gccgcgtttt catcggcttt tgtccgaatc
    12721 tctttcatat ccgttcgtgg atagtccacc agcgcatgtg ccacaccata ctgaaatgcg
    12781 aggctgaaaa attgctgcgc ccacacatcc agtcggctcc cctccatgtc gatattttct
    12841 gcatattccc tgatttttgc cggcgtttcc tcactcaata ctgtcggctc tgcaaatatg
    12901 cgcccaatat tttgcttgat gctttcttca tacacaggaa gtagcgtagc cacagacagg
    12961 cgttttttat aagcgtcttc atcttcgtta ggccattttg ggagataatt ttccccctgc
    13021 ctgcgcattt caagcgtacc gcccatcagt gcgtcgttaa tatcccacgc ctccagcata
    13081 tcgttatagt cgaggttggg tgttgatata tcagccataa ttaaatccga agtgatgtga
    13141 ctcttccggt cggtttgaca atagggaatt gcttaacgat gaaataacct ccggcatcat
    13201 tcgggtgatc attgccagat tttttatcag gctccccctt ctcatcccag acctgttgct
    13261 ccagagattc ggcatataac gggcaacgct tcacattaac tttatagcga cgctcaccat
    13321 tggcattgca gaacattgca ttcattgagt taacgcgatc ttttactggc gggttcgagc
    13381 tgttcaccac aacgttaaaa ccagcctgct taagctgggc tatatccgtc gtacttgcgt
    13441 tacttgattt tctggaatcc ccggaagcat ctggataaat ataaatttcc ctcactttcc
    13501 ggtaatcatt cccatcatac agccagaagc gttctttaat gatgcggatc atatccggcg
    13561 tatcgtaggc attgatgatt tcagttaccg cacatggaag ccccaaacgc agcacatgga
    13621 cgattcccgc catctttcca acgttaaaat ccatcccaat ataaatcggc tcccctggct
    13681 gctccacttc ttcgcaatta ttcagtttcc ggtcaaactg atggtaaaca gtaccacttg
    13741 tcaggttagt aaactgtcct cgaagatagg ctttaatcag ctctggaggg tatgattcaa
    13801 gaagcgaagg aatgtaatct gctggcaggt tcttttcatt atcgaaagta gatgcctgca
    13861 ccagaccata cagtgaggcc agctctgttt tttcacgcac ggctttaaca aactgctcgt
    13921 agacaaattt gaatccttcc ggcgtggttg taacgtcaat accgttgcga agtccatcaa
    13981 tcttataacg catacgtgca attatcttgc gccacgccgt tctggctttt tccttcggca
    14041 aaatgtccag ttcatccacc aacgcattac caattttgaa accgacgatc gtttgtggct
    14101 tctccatcga tctgcagata gtggttcccc gatactggcg tccgtaataa aagtgaacct
    14161 ctttatttcc ctcattaatt tttacgttca atccccagtc agcagcaact tcttccactg
    14221 tagggtaaaa aatatcgcga atttggggat acgttggcgc aaaatatccc tgatttatac
    14281 ctggatgctc ccaaatcccc ttgcatatgc cgccacaccc aacccatgtt ttgcccgagc
    14341 caaaaccagc aatataggct ttaaatttat ggggcattga aagaaatcgc gcctgaggca
    14401 cattaagcgt cggagagatc atcttcatca ctccttactc tggcatcaac cacattaata
    14461 ttgatcgcca caggctgggg atgttcatta tcttccaccg tttcgatctc tttgcgcagc
    14521 ttctggtttt ccattctgcg ccgttcaatt tccagttcct gtagccgctt atctgcacat
    14581 aaagccccgc cagcagaaag caaacgcaac aattcacgcc gggcggcagc cttatcctcc
    14641 agcaggatct caacgccgaa ttttccgaat tttgcccctg catataattg ccgcgcatcc
    14701 ccatcaagca gagtggtatc agccatataa agctgtcccg ttccctcacc gcagcacttc
    14761 gggcagtccg gattgggtat ggcgttatca acaaagccga ggcctccata ttccggttcg
    14821 ggtttgccat ctctggaagc ctgcgccgct gccttgtcga attctgctat atcgcgccac
    14881 tggtagagat gattctcgcc ccagcaataa cggcagttaa cacggcgaaa ttgtgcaagc
    14941 tgattggggt cggccaggac aatggccatc aactgactca ctagtaaatc caggtctgcg
    15001 gtatagcgtt tctggtactg attgcggaag tagctgatag cgcgaaaaac cctggcattt
    15061 ctaagcatac gactggcgtt gctattagct gtcgcacctt gcccctcata accggctagt
    15121 cggtacgcct ctgtcggctt tttaccctga gcaacaagca tcgcaaactt agcctgctgg
    15181 tcagaaatgc cgaattcatc gggacagaac gaaaattcct ccgcgtcgcc ctcattcagg
    15241 cccgcatcga atactggctt tttttcctga gatttttcgt tccgcttttg tgcagtctgc
    15301 gcagattttt tctgcgcact tttttgcgca gttttgcaca tttctgtctg cgcatttttc
    15361 ggagtttttt tgatgtaacg acgggctgtt gcgtaattca gtccccttgc ttcacaccat
    15421 gccaccggag atataccgga gcgggtgtat tcagcaatat actcctgctg caacgccccc
    15481 cagtccggtc tgctcatcag ttagtcctga tttttatcca ccctgagtag ttcgcgcaga
    15541 gcaaaggcat ccccttttct ggcaagctta aacaatgccg ctcgtaactc ggcttcacct
    15601 ttcgctctgc ccttacggat ggccgcataa aaatctgtca ttgcttcccg attttctttc
    15661 agtcggttca gatcaacatc cagaacgtca gcgatttgtt gtgcagtcat ccggcacgct
    15721 gccagagact cgactttcga atacggaatc atttgtcacc cccattgata tgcagggtgt
    15781 cttcttcctg tatttttcgt gaaggatttt tactgcagcg ttgttccagg tgacctgatg
    15841 gtgaatgcgt ttatggctgg cacccatcag tgagattttt acgcacgacg gcgcatacat
    15901 gacggagtaa aaacttttaa cgtaggttcc ggaatccaga tacagctcgg tcattccgcc
    15961 gctgtttttc tgcgtctgtt tctgccctaa ctggacagca ccgatcgtca taaacaattc
    16021 accacagcga ccgagattcg tgtacgtatt cacatcctcg ttaatgcgcc ccatgaatga
    16081 gaacggtcgt tcaaccgaac agataaagct gttcattgcc ttgcgtttca cccacgaagc
    16141 atggccgcca ttgtcaccaa gaaaatcccc gccctgcgac atagcgatgg aaagagcagg
    16201 tattgattcg tagtacgcca gcatttcaga aaggatcgca tccagtttcc ttatcggaaa
    16261 ataggcctgg tcatagttgc gatccacccg aaactggaac tcgtgataat catcatcgag
    16321 ctgaatgaag tatttacacc cgaccagttt tgccaggtcg aaacaggcat tacgggcgta
    16381 aaaaattgag cggcggtcac agaaattatc ggcttcgtca aaacgactgg cgatatcggc
    16441 tttggaaaac accagcacct gttccccaaa ttcagccatg tactgatgcc gtgtcttatc
    16501 ttcatcatca acaacgataa aaattttccc ggtatagcca gcacgacgca acgtccggta
    16561 agtcagaact ttgtccggtc gcccgtgagt cagaataaag gcgcaaaaat catcacgcat
    16621 attcctcctc ctcaccacca tgcatgatct ccaccatgcg ctgcgtcatc cggacaaatc
    16681 cattttcaat agcctgctga taatcaatga tcaccagcgc cgactcctcg aaaaaacact
    16741 gaatttcaga gggggcgtga gcgtaatagt ccgcaattct gctgaaatta aacaccgtgt
    16801 gacgttctgc cgcacacagg aggaatttct caatatcagg ctcaagggac gccgaacgta
    16861 tccggctgat cagctcctga gttttcgtat cgtcgtacag ttcactgata tccggtttac
    16921 cgcccgacgg ctcataaaca ggcgtatcaa ttttcgtcgt atacggctcc tcctcatttc
    16981 ctgtaccggg caaaacatcc gtcaacagtt catcaatttc tgtcgggatg aagcctgtca
    17041 gggagacatc aaaatcagca ttgattaggt ccgacagctc catccgcaac agatcttcat
    17101 cccagccagc attcatcggc aggcgattat ctgccaggcg gtacgccttt ttctgctcat
    17161 ccgtcaggcc agacagaaca atgaccggaa cagaatccat tttgagcatt tcagccgcca
    17221 taacacgacc gtgacccgca ataatttcgc ccttttcgtc aatcagcacc ggattagtcc
    17281 agccgaattg cttaatactt tctaccagtt gtgccacctg ctcagtactg tgcgtcctgg
    17341 cgttgtgcgc atacggtgac aattcttgta atgggcgata gactatcttt aatttctcgc
    17401 tcatacagcc ttgctttatg aataaaacgc accccagcag ccagtgctac tggggacgga
    17461 ggtgttgctg gtaaagttag gtattggatc aatgagtgag tcaacataat attaaactca
    17521 caattataaa tcagccatat attaggagcg ccaaaaaaaa cctgaaaaca atataataac
    17581 aggataaatt tcaaggcgac caagaatcat agctatgcac attaaacatt ttgcaatgtc
    17641 attaagcact ccgaatgacg atgcagtagc cccaaaacct aatcccatat tattaataca
    17701 tgcagccact gttgcaaatg atgtaagaaa atcatatccc ataccattta acaccagtat
    17761 aaaaaacacc gtgaagagag tataaagaaa aaagaaactc catacagacc tcattacacg
    17821 atctgtaact atcttccctc ctacatttac actcaacaac gctctgggat gagaaagctg
    17881 atttatctcg tgtttgcttt gtttgaaaag tataagaaat cgaagtgact taattccacc
    17941 acaagttgaa cctatacatc ccccaaagaa acttgacaac agcaaaaaca ctatcgtgtg
    18001 cgtgggccaa cttgcataat cctgcgtagc taaaccatta tcagtgagca tggagctggc
    18061 aagaaaaaac gaatgaataa aacttccatg caagtcatac atacctatat gccagacctg
    18121 gaaagaggta acaatgatca cccctaaggc tattaacaga aagaaacgaa gttcaatatc
    18181 tctgattaaa ggttttatcg ttttcctgct aataacaata taccaaagag tgaagttgaa
    18241 agccgatagc agggaaaaag aaccagccac cagctcaacc aaatagttat taaaatatcc
    18301 gatactctcg ctatgagttg agaaaccacc aagcgaaact gtggaaatcc cgtgacaaat
    18361 agcatcaaac aaaggcattc ctgcaagtct ataacagaca atacaagcaa tacctaataa
    18421 agaataagtt atccacagtg tccgtgacgt atcggccagg cggggagtga gtttgtcatc
    18481 cttaaatggc cccggcattt ctgactgata aagctttgca ccaccaatac ccaataatgg
    18541 caatacagca accgccagaa caataactcc taaaccacct ataaaattta actgtgaccg
    18601 atagtacaaa tatgcccgag gtaatgaact aacatcatca attacagttg ctcctgttgt
    18661 tgttattcca gaaacccctt caaacagagc atcaatgaac gttaaattaa gttctgagtc
    18721 aatccataaa gggaatgcac taataacaga aaacaaaatc caaaacatta caattataat
    18781 aaacccatca cgggtacgta attgaatgcc agatttctta gttgtatacc acgctccgcc
    18841 accaatgcaa aaaaatataa cgaaagttat aaagaaaacg aacaggcttt tttctttata
    18901 aaacaatgct acaaccattg gtggcaacat tgaaagacta tagagccaaa ccaggaaccc
    18961 acacatatga gtaacaactc ttacatgaga tgtattcata tctaaatatt ctttcaatta
    19021 taaccacctt gctgcaatat tatgattata ctgtataaaa tttaactcct cttagagctt
    19081 acttcactgt tccttatgaa acaatcatca aaatgaatca tattgtagtt aagattttac
    19141 tttaaacact gctcggttat gtattgctga gcaccttcaa gttgggcctg catcattacc
    19201 agtcgttccc ggagggtgaa ataatcccgt tcagcggtgt ctgccagtcg gggggaggct
    19261 gcattatcca cgccggaggc ggtggtggct tcacgcactg actgacagac tgctttgatg
    19321 tgcaaccgac gacgaccagc ggcaacatca tcacgcagag catcattttc agctttcgca
    19381 tcagctaact ccttcgtgta ttttgcatcg agcgcagcaa catcacgctg acgcatctgc
    19441 atgtcagtaa ttgccgcgtt cgccagcttc agttctctgg catttttgtc gcgctgttct
    19501 ttgtaggcga tggcgttatc acggtaatga ttaacacccc atgacaggca gacgacgatg
    19561 cagataacca gagcggagat aatcgcggtt actctgctca tacctcaatc tctctgaccg
    19621 ttccgccagc ttctttgaat tttgcaatca gactgtcagc cttatgctcg aactgaccat
    19681 aaccagcgcc cggcagtgaa gcccatatat tgctgcaacg gtcgatagtc tgacggatat
    19741 caccgcgatc aatcatcggc aaagcgccac gctctttaat ctgctgcagc gctacagcat
    19801 cctgactttc tggagaaaaa tctttcaggc caagttgctt gcggtaggca tcccaccagc
    19861 gtgaaagaag ctggtaacgt ccagcggctg ttgatttgag tttcggattt agcgtgacaa
    19921 gtttgcgggg gtgatcggag taatcagtga acagttcgcc accgacaata acatcataac
    19981 cgtgatttct ggttttctgc cgtccgttat ctgttccttc tgaccatgcc accatatcaa
    20041 ggaaagcttt acgctgggaa ttaagtgtct gcattaatta ctccttatgg gcaccgaact
    20101 tgttaccgat gaccctcatt gccgcaccac gaatagcatc aacaccaatc agccccaccc
    20161 caccaccaat ggcaacagaa agtgatttag gccatccgac atactcaagc gcggatgcaa
    20221 aggtcagcgt cagagcacca caaagcagaa tctcaagcgt ttttcgtttc cagccgccgc
    20281 caccgccaaa ataggcaatg cgcaaaccag ccataataat tgacataacc actgcaccca
    20341 gcggcgtatc tccacgccac caactttgta agagttccag taagtcaggc caggaatgag
    20401 gggcattgtg cattttcata agcctcacct ccgagagttc ggatggtgct aagtgtaaga
    20461 ttcaggctct caggcttgct aacaagaagt cgaggatgtt tccagagcct aacaacgaaa
    20521 aagccccggg acatgccggg gccagatgga gtgccagatt aagcttctgg cggtatatac
    20581 tcgtgtttga tatcgttaaa tcgccaaaag taacaattca aacaaagagg atttttatgt
    20641 ctgaaaaaaa caaaccacaa ggtgaaaata aacctcagca acccgtggca ccaaaaccaa
    20701 ctccaacaca aagtactaca gactttgcta cacgtcgtgt ttttgttgga gattctgccg
    20761 actcagtcat tgaacatata aaaaaacagc cgagataaac atcgccgcta ccggagcaag
    20821 gatggtatac atccttgctt tatcgagact cgtgcggatt ttctcatttt ccaacagtaa
    20881 ctctcttgct gtatcactca agtcaacaag gcgatacctt cgtataagcg gcaataactt
    20941 atcaggtcct aaatatcctg catcagcgaa tattttaaag ctcgagggct ccatatcctt
    21001 atatttttca tgatatagat gatcaggagg ggcattgatc aggcccctaa ccttcacaga
    21061 taaaccagta cataccaagt aaatggcgca ccatgtccat agtaatgtaa atgtggtaat
    21121 tccggcggtg agaaaatcga aattagtttt ctgtgtcagc aataaaaaag atgaaccaat
    21181 tccaacaatc tgaatgttca gaagtttgta tccattctca acattggttt tgttagaaag
    21241 atgaatctct cgtatcgtct cttccccttg tttttcaaga taatcgacga gctcatcatc
    21301 tactcctaaa aaataatctt taggtagttc tctcatctca cctccacatc ctgtactgaa
    21361 aacaatttta ccagaatgtc tcgattctag gtattccgcc aggaatcgcg ctccagaaat
    21421 gaaacatcag gttcaccagt accagaaaca acaaaacccg ctcaatggcg ggttctggta
    21481 aagttcatgc gcttggttcg cctcgcgata cagctttgcg aagcaaaccg gaattgaagc
    21541 agtttatgcg taaaaaatca agctattttt tgagcaaatg attctcgcat gggaatatat
    21601 agggcatact cagcaacagc caaccaatta gcaattcgct tttcgcatgt gctaaaacac
    21661 cactcaggat gtgcatcatt tagcaattca gccattttgc gcttagtcat cccccgtcct
    21721 tcataccgtt gccggaggac actaatcaat ccaggatgct ctgccagcac ctcacttatg
    21781 actcgatcaa tacataacgc ctctgcatca gtacaatgcg ccagccagct cttttgcttg
    21841 ccgttgatca tctctcgcaa aaacgcttcc agctcagctt tctctattcc cgcttttttc
    21901 attctgcgca gggcttcatt gatggctgtt ttcgtcaatt ttttggatgc caacaactga
    21961 ttgaacatat ttcctgacct gccaccgcca atatacgacc agcgccccca catacgcagt
    22021 ttgccctgaa tccagacact ttccagcgtg gcgagacgaa ggtgttctcc gctttttcct
    22081 gtattcgttg ggtaaatcac aaatatccct cctttctcca gatttcttgt gtgcgaaaaa
    22141 caccttctgc atgcatcagg cgtaattctt ctttggtgta atcgctggtt tttacccgcc
    22201 cgtcgattag atcgtggcat gagctacagg caatcgccgc ctgcatatcg tgtggttttg
    22261 tcgctgttcc gcacgttccc gccagtcggt aatgcgccag cacagacgtt tccggatcgt
    22321 gattgcagta gccaggaatt ctgacggtgc acatctgccc ccgcgccgct ttacgtaaat
    22381 ccaccattac gcaaactcca gcagctgcgc ggtcacattt tcgacttgtt ccggagagga
    22441 aaatttacgg aacagaatcc agttccacag cacattcaga acagatttat aaacctgctg
    22501 aaactcggtt tcgtccatat tcgcaaaagc gatggatttc gcccgacgcc catggctacc
    22561 atcaggataa agatgctcgg tgtaaaatcc ggcctgaatg gttacccact cgcggaaagc
    22621 ctcaaacgac tttagcaatg ccgtatcccg ggttctgcgt gtcgcaactg tattcagata
    22681 ttgctctgcg gcttcgctca gagctggcgt atgttcccgg cctactgatt cacataggta
    22741 atcaacgaat cctgaaacca gctttcgttc gcgaggcgtg atcgccccac cgaccggagt
    22801 ccagtaatcg aatcccagtt gcaggagttt gaaaaaacgc ttgtggaatg cgtagttacg
    22861 cacacgctta aagtctgcgt gtatccactc gcctattttg atttgatgca aaaaatcgca
    22921 actctccggc gtcgccggga gaagtagtcc agaagaggtt tgtttgacca gttgtatatg
    22981 cgccatttct caatctctcg atggcgcagt gcagcagatg ccagttgttc aggctgacga
    23041 ataaagtata aataaactgg ctatggtgta aagctccaca taacatgaac aaacactaca
    23101 tatcaaatag ctgatacaag gatagaaata caacacttat tattaaaaac gattagataa
    23161 attacatttt aatgttatga aaaagttctt ttttatcata acatttcaac aaaagcatta
    23221 cagatgcaca tcccgtcatc atcaattatt taaggtggtt aaacatggaa aataacaact
    23281 atgcacatct cgctcctttt ttatccgtaa tccttttcgc tggctgtttt atatgggcat
    23341 tatttttata aagtagccgc atgattagtt tctggaatca tgtttcccac tcaaatcatt
    23401 aacatttgat aagatatatc taaaaggatg cctttacatt atttgatgcg tatatgattt
    23461 atttatatgc acagtaaagg catcctggaa ataatcaaga ttaatgatat attgcttatc
    23521 tcatccccca ctgtctttca cccggaaaac atttatcgtt aaaaatatat cataagtaat
    23581 taaattgaaa tttcagaaca caactaatgc acacaataag attcagtaac agtaatctat
    23641 gcggactata tcgagatttt ttagttaaac tttaataagt tattcctaac acacagaatg
    23701 cacaaactaa aagtaaaaca aatagttata cgaaaaacaa ccatattgat tctatcttct
    23761 cctctaatca acatatcaat tatgtgtttc ataacagctt gacataaccc tcaaaaaggt
    23821 gcatttataa tgcatgtttt atgggaggtg gttatgacac acaagagaat tcctaaagac
    23881 tgggtaatca aacgctcaac tccgttcttc acaaaagaga acgtaccttc agcgttatta
    23941 acacatcata atacagcagc aggtgttttt ggacagttat gcgtgatgga aggtactgta
    24001 acatattatg gttttgctga tgagaatgct actgaaccgg agataaaagt agtcattaat
    24061 gctggcgctt ttgcaacaag cccaccacaa tactggcacc gtgttgaact aagcgatgat
    24121 gctcagttta atattaactt ttgggtagct ccagacttct caggcgaaaa agtctatacc
    24181 gcaaaaaaag aatagccacg ctataaatat cttattaact agtcccggcg tggttctttc
    24241 cccgctgggt cttttactca gattagtagt ctacgaatca gagcctccgt ctgcc
//
LOCUS       DAPOZO010000073        24158 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPOZO010000073 DAPOZO010000000
VERSION     DAPOZO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.073"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            32..352
                     /gene="trbA"
                     /locus_tag="ROI21_003209"
     CDS             32..352
                     /gene="trbA"
                     /locus_tag="ROI21_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HDX5112099.1"
                     /translation="MTCLFAPSLIFVIIAGYFFSERNDRKMFLLSSLVFLVINIACLY
                     VLTASLWFLCGAIMNQGAAMVVPMVIGSLPNVTSFDRFRRIFICIMLSSAWSGVMWFF
                     IRGL"
     gene            479..763
                     /gene="traQ"
                     /locus_tag="ROI21_003210"
     CDS             479..763
                     /gene="traQ"
                     /locus_tag="ROI21_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HDX5112100.1"
                     /translation="MISKCRFSLPRLDITGMWVFALGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            750..1295
                     /gene="trbB"
                     /locus_tag="ROI21_003211"
     CDS             750..1295
                     /gene="trbB"
                     /locus_tag="ROI21_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HDX5112101.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            1225..1572
                     /gene="trbJ"
                     /locus_tag="ROI21_003212"
     CDS             1225..1572
                     /gene="trbJ"
                     /locus_tag="ROI21_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HDX5112102.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            1520..1945
                     /gene="trbF"
                     /locus_tag="ROI21_003213"
     CDS             1520..1945
                     /gene="trbF"
                     /locus_tag="ROI21_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HDX5112103.1"
                     /translation="MNHQKYQRELMMKEKINDTEPGIKQIEREIERGCDNAKKYFWLF
                     VVFFAAGLIVRNVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLA
                     AASLSPLCEIIFGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            1932..3305
                     /locus_tag="ROI21_003214"
     CDS             1932..3305
                     /locus_tag="ROI21_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HDX5112104.1"
                     /translation="MLRIKPLLVLCAALLTFTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMSYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            3302..6121
                     /gene="traG"
                     /locus_tag="ROI21_003215"
     CDS             3302..6121
                     /gene="traG"
                     /locus_tag="ROI21_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HDX5112105.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAMESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWSDDDAHQASSGSRASNDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQIQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSV
                     QERMPGADSPQELMKRAKEYQDKYK"
     gene            6140..6637
                     /locus_tag="ROI21_003216"
     CDS             6140..6637
                     /locus_tag="ROI21_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HDX5112106.1"
                     /translation="MIMRCLAHIALVTVIQFIACYLVGWGKAETVFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCI
                     SIKKWGQNQLKNQYDGSEKLDFGGIEQPPTTYYNPSTGYPMHGGFDSAGNTFGTRWQD
                     YYDRQ"
     gene            6669..7400
                     /locus_tag="ROI21_003217"
     CDS             6669..7400
                     /locus_tag="ROI21_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HDX5112107.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPDSERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            7603..8382
                     /locus_tag="ROI21_003218"
     CDS             7603..8382
                     /locus_tag="ROI21_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112108.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKKLTEILGKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVLCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQGENITWSLSSQS
                     PADKYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRAAGGYHHRHTGYTWSVSHIILFCPVPDFPGVSS"
     gene            8379..10604
                     /gene="traD"
                     /locus_tag="ROI21_003219"
     CDS             8379..10604
                     /gene="traD"
                     /locus_tag="ROI21_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDX5112109.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLI
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQ
                     PQQPQQPQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEM
                     EQQLPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDD
                     F"
     gene            10604..15874
                     /gene="traI"
                     /locus_tag="ROI21_003220"
     CDS             10604..15874
                     /gene="traI"
                     /locus_tag="ROI21_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HDX5112110.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFPPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTEAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDEPRTAEKLARHPSFTVVSDQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGTSETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGRGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTELAVRDIAGQERDRTASSERETALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            15894..16640
                     /gene="traX"
                     /locus_tag="ROI21_003221"
     CDS             15894..16640
                     /gene="traX"
                     /locus_tag="ROI21_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HDX5112111.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            16695..17255
                     /locus_tag="ROI21_003222"
     CDS             16695..17255
                     /locus_tag="ROI21_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HDX5112112.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            17386..17598
                     /locus_tag="ROI21_003223"
     CDS             17386..17598
                     /locus_tag="ROI21_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112113.1"
                     /translation="MNGFRNSSRNGQVWCWQRAGSRAVILEVSGRRMEAAEAWRRAAG
                     VAPRTDWQQFARKRAAHCHRRCRGRG"
     gene            18111..18397
                     /locus_tag="ROI21_003224"
                     /pseudo
     CDS             18111..18397
                     /locus_tag="ROI21_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766809.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
     gene            18469..18603
                     /locus_tag="ROI21_003225"
     CDS             18469..18603
                     /locus_tag="ROI21_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDX5112114.1"
                     /translation="MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA
                     "
     gene            18900..19154
                     /gene="repA"
                     /locus_tag="ROI21_003226"
     CDS             18900..19154
                     /gene="repA"
                     /locus_tag="ROI21_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HDX5112115.1"
                     /translation="MSQTENTVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCNEDGLTQAEVLTALIKSEAQKRCM"
     gene            19260..19394
                     /locus_tag="ROI21_003227"
                     /pseudo
     CDS             19260..19394
                     /locus_tag="ROI21_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615301.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein CopA/IncA"
     gene            19391..19465
                     /gene="tap"
                     /locus_tag="ROI21_003228"
     CDS             19391..19465
                     /gene="tap"
                     /locus_tag="ROI21_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HDX5112116.1"
                     /translation="MTGKVQDFFLCSLLLCNISAGWCD"
     gene            19458..20315
                     /gene="repA"
                     /locus_tag="ROI21_003229"
     CDS             19458..20315
                     /gene="repA"
                     /locus_tag="ROI21_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDX5112117.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFSSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKFSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTLALFAALDV
                     SEDAVAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRKRQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            21017..21157
                     /locus_tag="ROI21_003230"
     CDS             21017..21157
                     /locus_tag="ROI21_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112118.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLP
                     CR"
     gene            21255..21908
                     /locus_tag="ROI21_003231"
     CDS             21255..21908
                     /locus_tag="ROI21_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDX5112119.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            22001..22258
                     /gene="pemI"
                     /locus_tag="ROI21_003232"
     CDS             22001..22258
                     /gene="pemI"
                     /locus_tag="ROI21_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HDX5112120.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            22191..22592
                     /gene="pemK"
                     /locus_tag="ROI21_003233"
     CDS             22191..22592
                     /gene="pemK"
                     /locus_tag="ROI21_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HDX5112121.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            22877..>24158
                     /locus_tag="ROI21_003234"
     CDS             22877..>24158
                     /locus_tag="ROI21_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDX5112122.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHAIHTRTQ
                     LENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRYYQAVFL
                     ALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLCALIRRY
                     FKESENKFINHAWQTLIRTLLNNSRTEQPNYDFKQ"
BASE COUNT         6082 a         5650 c         6765 g         5661 t
ORIGIN      
        1 aattttaatc agcgagtcag cttgggaaga aatgacctgc ttattcgcac cttccttaat
       61 atttgtcatt attgccggtt atttcttttc tgagcgtaat gacaggaaaa tgtttctcct
      121 gagctcactg gttttccttg ttattaatat cgcgtgttta tatgtgctta ccgccagtct
      181 ctggtttctg tgtggtgcaa ttatgaatca gggcgcagca atggttgttc caatggttat
      241 tggctcatta ccgaacgtta cgagcttcga caggttcaga agaatattta tctgtattat
      301 gttgtcatca gcatggtccg gagtgatgtg gttttttata aggggcttat gacaggctaa
      361 gtcatataaa acggtaataa aaaccataca aaataatgag gttgatctga ttaatatcac
      421 acaaacgcga atattacaga catgaatatt ctcatgtaaa taataaccgg agtttaacat
      481 gataagtaaa tgcagattct ctttaccccg gcttgatatt acgggaatgt gggtatttgc
      541 tctgggtgtc tggtttcata tcgtcgcccg tcttgtttac agcaaaccct ggatggcttt
      601 ttttctggcg gagttgattg cggccatcct tgtgctgttc ggcgcatacc aggtgctgga
      661 tgcatggatt gcacgtgtca gcctggaaga gcgtgaggca ctggaagcca ggcaacaggc
      721 catgatggaa gggcagcagg agggcggaca tgtctctcac taaattactg ccggtcattt
      781 tgttgatgat ggccacaggt gtgcaggcct ccacccggga tgaaattgaa cgactctgga
      841 atccgcaggg tatggccgtg cagcctgcac aaccggcagc agacacatca gccagaacag
      901 aaaagccggc tccccgctgg ttccgcctca gcaacggcag gcaggttaac ctggccgact
      961 ggaaggtggt gctgtttatg caggggcatt gcccttactg tcaccagttt gacccggtac
     1021 tgaaacagct ggcacagcag tacggctttt ctgtttttcc ctacaccctg gatggtcagg
     1081 gtgataccgc ctttccggaa gcattaccgg tgccaccgga cgtgatgcag accttcttcc
     1141 cgaatatccc ggtggccaca ccgaccacct tcctggtcaa cgtcaacaca cttgaggcat
     1201 taccgctttt gcagggggca acggatgccg ccagttttat ggcgcggatg gataccgttc
     1261 tgcagatata cggagaggaa aaaggtgcga aataagcagg tggtgttact cattgccgga
     1321 atatccggca tcgcgacggg aataatcgtc agcctgaata tcccctttat ccgtcagggg
     1381 cttttttatc ccgccagccc tgtggaaatt gtcgtttcgc tgtgtcttac cttttctgtt
     1441 tctgttgtgt tttttgtggg ggcaattgtg ggatggattt cggtgtctga aatatattac
     1501 agccgcatga cctgtctgca tgaatcatca gaaatatcag agggaactta tgatgaaaga
     1561 aaaaataaat gatacagaac cgggaatcaa acaaattgaa agggagattg aacgtggttg
     1621 tgataatgca aaaaaatact tctggctttt tgtggttttc tttgctgccg gattgattgt
     1681 aaggaacgtt atgcatgatt ttttcagtgc cggtattgat tcgtggaaag ctgacccgga
     1741 attaaataat tttcgttata tgtggaacac cctgatgtat gtcattccga ttatgttata
     1801 tgcgcttgca gctggttttc ttgctgcggc ttctctgtcg ccgttgtgcg aaataatatt
     1861 tgggggtgtc cggatattcc tgctgaaacg ccgtatgcgc cgggagaaca cattaaggga
     1921 gggcagcaat aatgcttcgc attaaacctc ttctggttct ttgtgccgct ttactgacgt
     1981 tcacacccgc agcgtcagcg gatgtgaaca gcgatatgaa tcagttcttt aacaagctgg
     2041 gcttcgcctc caataccaca cagccgggcg tctggcaggg gcaggccgcc gggtatgcct
     2101 atggtggctc cctgtatgcc cgcacccagg tcaaaaatat ccagctgatt tccatgacgc
     2161 tgccggatat caatgccgga tgtggcggca tcgacgccta tctcggctcg ttcagtttta
     2221 ttaatggcga tcagttgcag cgttttgtta agcagattat gagcaatgct gccggttact
     2281 tttttgacct tgccctgcag acaacggtgc cggaaataaa aaccgcaaaa gacttcctgc
     2341 agaaaatggc aagtgacatt aacagtatga acctcagttc ctgtcaggtg gcacaggggc
     2401 ttgtgggggg gctgttccct cagacgcagg aatctcagca gaaagtttgt caggacattg
     2461 gcagtgaaag caatatcttc gccgactggg ccgcatctcg tcaggggtgc acggtcggag
     2521 ggcagtcctc atctgttcgt gacaaagcca gcgacaagga caaggagcgg gtgctgaaaa
     2581 accagaatat catatgggag gcgctgaaaa aaaacagaat gtttgacggc aacaaagagt
     2641 tgaaagaatt tatcatgacg ctcaccggta cgctgatttt tggggaagac agcgagatta
     2701 cccccctgcc ggcgcgtacc actgaccgcg atatcctccg ggcgattatg gaggggggaa
     2761 cggcaaaaat ttaccactgc aacgattcgg acaagtgtct gaaggtggtg gcggatgcaa
     2821 atgtgaccat cgcccgggat aaagccctga agtcacagat tacaaaactg ctcaccagta
     2881 ttcagaataa agcagtgagt gacactccgc ttgacgcccg ggaacagggg tttatttcca
     2941 gcaccaccat tcccgttttt aaatatctga ttgacccgca gatgctcggt gtgtccaaca
     3001 gcgtcgttta ccagctgacg gactatatcg gctacgacat catgctccag tacattcagg
     3061 aactgcttca gcaggcccgg gccatgattg ccaccgggaa ctatccgcag gctgtgatgg
     3121 acaatgttct ggagaatctg aatcaggcac agcaacagat tgccattttc cagagccagg
     3181 tacaggtcca gcaggatgcg ctgctggttg tcgaacgtca gatgagctac atgcgccagc
     3241 agctttccgc ccgtatgctc agccgttacc agaacaacta tcacttcgga gggaattcac
     3301 tgtgaatgaa gtttatgtga ttgccggtgg tgagtggttg cggaataacc tgaacgccat
     3361 tgcggcattt atgagtacgc ggacctggga ttccatcgaa aaaattgcgc tcacattgtc
     3421 tgttctcgcg gtggccgtga tgtgggtaca gcggcacaac gtgatggatt tgctgggctg
     3481 ggtggccgtg tttgtgctta tcagcctgct ggttaatgtc cgtacatcgg tgcagattat
     3541 tgataacagt gacctggtca aagtccatcg ggtggataat gtgccggtcg gtctggcgat
     3601 gccactttca ctgacgacac gtatcgggca tgcaatggtg gtcagctacg agatgatatt
     3661 cacccaaccg gacagcgtca cctacagcaa gacaggcatg ctgttcgggg cgaatctgat
     3721 tgtgaaaagc accgatttcc tgtcccggaa tccggaaatc atcaatctgt tccaggacta
     3781 cgtccagaac tgcgtgctgg gtgatattta cctgaatcat aaatacacgc tggaagatct
     3841 gatggcctcc gctgacccct acacgctgat tttttcccga cccagcccgc tacggggcgt
     3901 ttatgacaac aataataatt tcataacctg taaggatgcg tcggtcacgc tgaaagacag
     3961 gctgaatctc gatacaaaga cgggaggcaa gacctggcat tattatgtgc agcagatatt
     4021 tggcggcaga ccggacccgg acctgttatt cagacaactg gtcagtgaca gttacagtta
     4081 tttctacggc tccagtcagt ctgccagcca gattatgcgc cagaacgtca caataaatgc
     4141 cctgaaagag ggtatcacca gtaatgccgc ccgtaatggt gacaccgcca gcctggtgaa
     4201 tctggccacc acgtcatcga tggagaagca acgtctggcg catgtctcta tcggtcatgt
     4261 gacaatgcga aacctgccaa tggtacagac tatcctgacg ggaattgcca ttggtatttt
     4321 cccgcttctg gtactggcag ctgtcttcaa caaactgaca ctgtccgtcc tgaaaggcta
     4381 tgtgtttgcc ctgatgtggc tgcagacctg gccgttgttg tatgccattc tgaacagtgc
     4441 catgacattc tatgcgaaga tgaatggtgc gccggtcgtg ctgtctgaac tctctcagat
     4501 acagctgaaa tactctgacc tggcctctac tgccgggtat ctttcagcca tgatcccccc
     4561 gttgtcatgg atgatggtaa aaggccttgg cgcaggtttt tccagcgtgt acagccactt
     4621 cgcctcttcc tctatcagtc cgacggccag tgcagcaggc agtgtggttg acggtaatta
     4681 ctcctacggc aacatgcaga cggaaaacgt gaacggcttc agctggagca ccaacagcac
     4741 cacgtcgttt ggtcagatga tgtaccagac cggcagtggc gcaactgcca cacagacccg
     4801 tgacggtaat atggtgatgg atgcaagcgg agcgatgtcc cggttaccgg ttggtatcaa
     4861 tgcaacgcgt cagattgcag cggcacaaca ggaaatggcc cgggaggcgt cgaacagagc
     4921 agaaagtgcc ctgcatggtt tcagcagcag tattgccagt gcctggaaca cgctcagtca
     4981 gtttggttct aaccggggga gcagtgattc tgtcaccggt ggcgctgaca gcaccatgag
     5041 tgcacaggat tccatgatgg ccagccgtat gcgcagtgca atggaaagct atgcgaaggc
     5101 gcataatatc agtaatgagc aggcgactca ggaactggca tccagaagta cgagagcttc
     5161 tgccgggatg tatggcgatg ctcatgctga atggggggtt aaacctaaaa ttcttggtgt
     5221 tggtggagga ttaggcgtta gaggcggtgg tcgggctggc attgactgga gtgatgatga
     5281 tgcgcatcag gccagcagtg gttcacgggc cagcaatgat gctcgtcatg atatcgatgc
     5341 cagagctaca caagacttta aagaggccag cgattacttt accagtcgca aggtcagtga
     5401 gtccggcagc catactgaca ataatgctga ttcccgtgtg gatcagttgt ctgcagccct
     5461 gaattcagcg aagcagagtt acgaccagta cacgacgaat atgactcgca gccatgaata
     5521 tgctgaaatg gcatcccgta ctgaaagcat gagtgggcag atgagtgaag acttgtcgca
     5581 acagtttgca cagtatgtaa tgaaacacgc gccacaggat gcagaagcta ttctgacaaa
     5641 taccagttca ccggagattg cagaacgccg tcgggcgatg gcctggtctt ttgtgcagga
     5701 acagatacag cctggtgttg ataatgcctg gcgggaatcc cgtggcgata ttggtaaagg
     5761 aatggagagc gtaccttcgg gtggtggcag tcaggatatt attgctgacc atcagggaca
     5821 tcaggccatt attgagcaaa gaacgcagga cagtaatatc cgtaatgatg taaaacatca
     5881 ggttgataat atggtcacag aatataaagg gaatataggt gatactcaga acagcatacg
     5941 tggcgaggaa aatattgtaa gaggacagta ttctgaacta cagaatcacc ataaaacaga
     6001 ggcattatct cagaataata aatataatga agaaaagtcg gttcaggaaa gaatgcccgg
     6061 ggctgacagt ccacaggaac tgatgaaaag ggcaaaggag tatcaggata agtataagta
     6121 ataatgctgt gtttgggaga tgataatgag atgtctagcc catattgctc tggtaacggt
     6181 gatacagttt atcgcctgct atctggttgg ttgggggaag gccgaaacag tttttatgct
     6241 gttcttcata gtgctatggc agggactgtt tatatggttg ttctctcaaa tacgaaaaaa
     6301 acggaatgtg tcggatgagt ttaaattcag taaaggcgtt tggtatataa cgatgccagt
     6361 atcttcctta ctctctccgt tattaagcct gatggttttt atcattggta cactatacga
     6421 attaaggcga gttagtggtt gcataagcat aaaaaaatgg ggtcaaaatc aactcaaaaa
     6481 ccaatatgat gggagtgaaa agttagactt cgggggcatt gaacagcccc caacgaccta
     6541 ctataaccct tcaaccggat atcctatgca tggaggcttt gactcagcgg gaaatacttt
     6601 tgggactcgt tggcaggatt attatgacag gcaataagaa aactgtagtt tgtaaaaaag
     6661 gtaatttgat gaaaacaaaa aaattaatga tggttgcact ggtcagttcc actctggccc
     6721 tttcagggtg tggtgcgatg agcacagcaa tcaagaagcg taaccttgag gtgaagactc
     6781 agatgagtga gaccatctgg cttaaacccg acagcgaacg cacggtattt ctgcagatca
     6841 aaaacacgtc tgataaagac atgagtgggc tgcagggcaa aattgctgat gctgtgaaag
     6901 caaaaggata tcaggtggtg acttctccgg ataaagccta ctactggatt caggcgaatg
     6961 tgctgaaggc tgataagatg gatctgcggg agtctcaggg atggctgaac cgtggttatg
     7021 aaggcgcagc tgttggtgca gcgttaggtg ccggtattac cggctataac tcaaattctg
     7081 ccggtgccac actcggtgtg ggccttgctg ctggtctggt gggtatggct gcagatgcga
     7141 tggtggaaga tgtgaactat accatgatca cggatgtgca gattgcagag cgtactaagg
     7201 caacggtgac aacggataat gttgccgccc tgcgtcaggg cacatccggt gcgaaaattc
     7261 agactagtac tgaaacgggt aaccagcata aataccagac ccgtgtggtc tcaaatgcga
     7321 acaaggttaa cctgaaattt gaagaggcga agcctgttct cgaagaccag ctggccaaat
     7381 caatcgcaaa tattctctga ttatcttccg gaggctggtc tgaccggcct cctgactgac
     7441 gcctcgcttt gctcgttgtc caaccccgga aagaaataaa ataaatttta tttctccccg
     7501 gcgtttccga aataaatatg aaaacactga gcggttgtgc aaattctttg tgcgaccgtg
     7561 aagtgttgcc ggcgaagccg ggatcttccg gagaaagtca ttatgacatc agacaatatc
     7621 gctgaatatg ttggtgagaa taatcaattt actgagaagc ctgtaactga ataccccccg
     7681 gtgatacagg ataatacagt gaccactaaa gactatctgt acagcaataa tattgatact
     7741 gaccttttag tggttatcgg agggcttatt gttatattgt ttctcatgat gcttatcatg
     7801 tactttatta attctctcag gcataagaaa ttaactgaaa tactgggaaa aatatctttt
     7861 cctgaaaaat ccgggagcaa tttgcttaaa ccggaggcgg tcagctgtaa tttgttgtcc
     7921 ctgtgtgtgc tggcaccggt actctgtaca cccagaaagc tggcagaagt ctccagggag
     7981 atcatcgtgg cgagtgcgca ggaaattgca tggacgccgg ggagcagtcg tattgatggt
     8041 catcagggcg aaaatattac ctggtctctg tcatcgcagt ctcctgccga taagtatgat
     8101 ctcatcaata aaacgtcccg tgtttctcct gacagtggcc gtttttatga accggacaca
     8161 gttctggatc accgtgaacg ggtacgcctt tttattcaga tccagtttcc gcacgggagt
     8221 gactggtctg aagtattgcg cctgactgac attgttacag agaaggtctc ttccggcagg
     8281 gctgcaggcg ggtatcacca ccggcataca gggtacacct ggtctgtttc ccacatcatc
     8341 cttttctgcc ctgttccaga ttttcccgga gtttcatcat gagttttaac gcaaaggata
     8401 tgacccaggg cggtcagatt gcgtccatgc gtatccgcat gttcagccag atcgccaata
     8461 tcatgcttta ctgcctgttt atttttttct ggatactcgt tggtctgatt ttatgggtaa
     8521 aaataagctg gcagacgttt gtgaacggct gtatttactg gtggtgtacc acgctggaag
     8581 gcatgcggga tttaatcaag tcccagccag tatatgagat tcagtattac ggcaaaacct
     8641 tccggatgaa cgctgcccag gtactgcatg ataaatatat gatctggtgc ggtgagcagc
     8701 tatggtccgc attcgttctg gcgtctgtcg tggccctggt tatttgcctg atcaccttct
     8761 ttgttgtctc ctggattctg gggcgtcagg gtaaacaaca gagcgaaaat gaagtcacag
     8821 gtggtcgtca gctgacagac aatccgaaag acgttgcccg gatgctgaaa aaagacggca
     8881 aggactccga tatccggatt ggcgacctgc cgattatccg ggattccgaa atccagaact
     8941 tctgtctgca cggcacggtc ggggccggta agtcggaggt tatccgtcgt ctggccaact
     9001 atgcccgtca gcgcggtgat atggtggtga tttatgaccg ttcaggggaa tttgttaaaa
     9061 gttactatga tccctccatc gataaaatcc tgaatccgct ggatgcccgc tgtgccgcat
     9121 gggatttgtg gaaggagtgc ctgacacagc cggattttga taataccgca aatactctga
     9181 tccctatggg tacaaaagag gacccgttct ggcagggttc aggacgtacc atttttgcgg
     9241 aagcggcgta cctgatgcgt aatgacccca accgcagcta cagcaaactg gtggacacac
     9301 tgctttccat caaaattgaa aaactgcgta ccttcctgcg taattcaccg gcggccaacc
     9361 tggtggaaga gaaaattgag aaaacggcga tttccatccg tgctgtgctg accaactacg
     9421 tgaaagccat ccgttacctg caggggattg agcataacgg tgagcccttc accatccgtg
     9481 actggatgcg tggtgtccgg gaagatcaga aaaacggctg gctgtttatt tcgtcgaacg
     9541 ccgacaccca tgcctccctg aagcctgtga tctccatgtg gctgtccatt gccattcgtg
     9601 gtctgctggc aatgggagaa aaccgtaacc gtcgtgtgtg gtttttctgt gatgagttac
     9661 ccacgttaca caaactgccg gacctggtgg agatcctgcc ggaagcccgt aagttcggtg
     9721 gctgttatgt gtttggtatc cagtcctatg cccagctgga agatatctac ggtgagaaag
     9781 cggcggccac gctgtttgac gtcatgaaca cccgtgcttt tttccgttcc ccgagccata
     9841 agattgcaga gttcgctgcc ggtgagattg gtgagaaaga gcacctgaaa gccagcgagc
     9901 agtattccta cggtgctgac ccggtacgtg acggggtatc gaccggtaag gatatggagc
     9961 gtcagacact ggtcagttat tccgacattc agtctctgcc ggatctgacc tgctatgtca
    10021 ctctgcccgg accgtatccg gcagtaaaac tctccctgaa atatcaggca cgaccgaagg
    10081 tcgctccgga gtttattccg cgtgacatca acccggaaat ggagaaccgt ctgagtgccg
    10141 tacttgctgc aagggaagca gaaggtcgcc agatggccag cctcttcgaa ccggaggttg
    10201 cctccggaga aggcgtgact caggctgaac agccacaaca gccacaacag ccacaacagc
    10261 cacaacagcc acaacagcca caacagccac aacagccaca acagccacaa cagccacaac
    10321 agccagtgtc ttctgtcatc aatgataaga agtcagatgc aggtgtgagt gttccggcag
    10381 gggggatcga gcaggagctg aaaatgaaac cggaagaaga gatggaacag caactgccac
    10441 ccgggatcag tgaatccggt gaagtggtgg atatggccgc ttatgaggca tggcaacagg
    10501 aaaatcatcc ggacatccag cagcagatgc agcgtcgtga agaggtgaac attaatgtgc
    10561 accgggagcg cggggaggat gttgagccgg gagatgattt ctgatgttga gtttcagcgt
    10621 tgttaaatcg gcaggcagtg ccggaaacta ttacaccgac aaggataatt actatgtgct
    10681 gggcagcatg ggagaacgct gggccggtca gggggctgaa cagcttgggc tgcagggcag
    10741 cgtcgataag gatgttttta cccgtcttct ggagggcagg ctgccggacg gggcggattt
    10801 aagccgcatg caggatggca gtaataagca tcgtcccggc tacgacctga ccttctccgc
    10861 ccccaaaagt gtctccatga tggccatgct cgggggggat aaacgtctta ttgatgcaca
    10921 taaccaggct gtggattttg ctgtccgtca ggtggaggcg ctggcttcca cacgggtgat
    10981 gacggacgga cagtcagaaa cggtgctgac cggtaatctg gtgatggcac tgtttaacca
    11041 tgacaccagt cgcgatcagg acccacagtt acacacgcat gtggtggtgg ctaatgtcac
    11101 gcagcataac ggcgaatgga agacactgag cagtgacaaa gtggggaaaa ccggattttc
    11161 cgagaacgtg ctcgcgaacc ggattgcgtt cgggaaaata tatcagagtg agttacgtca
    11221 gcgcgttgag gcgctgggtt atgaaaccga agtggtcggt aagcacggta tgtgggagat
    11281 gccgggcgta ccggtggaag ccttctccgg acgctcacag gctatccggg aggccgtcgg
    11341 tgaagacgcc tcgctgaaat cccgggatgt ggcggcactg gatacgcgta aatccaaaca
    11401 gcacgtcgat cctgaagtca gaatggctga atggatgcag acgctgaagg aaaccgggtt
    11461 cgacatccgg gcatatcgtg acgcggcgga tcagcgtgcg gagatccgca cgcaggcgcc
    11521 cgggcctgct tcacaggacg ggccggatgt gcagcaggcg gtgacacagg ccattgccgg
    11581 attaagtgaa cgaaaagtgc agttcacgta tacggacgtg ctggccagga cggtcggcat
    11641 actgccgccg gaaaatggtg tgattgaacg ggcacgcgcc ggtatcgatg aggccatcag
    11701 ccgtgagcag cttatccccc tcgatcgtga gaaggggctg ttcacgtccg ggattcatgt
    11761 gctcgatgag ctgtcagtcc gggcactcag tcgtgacatc atgaaacaga accgggtgac
    11821 cgtacatccg gagaaaagtg tcccccggac ggccggttac agcgatgccg tcagtgttct
    11881 ggcacaggat cgcccgtcgc tggccattgt gtccgggcag ggcggtgcag ccgggcagcg
    11941 tgagcgggtg gctgaactgg tcatgatggc ccgggagcag gggcgggagg tgcagattat
    12001 cgctgctgac cgtcgctcgc agatgaacct gaagcaggat gaacggctgt ccggtgaact
    12061 gataaccgga cgtcgtcagt tgctggaagg catggccttc ccgccgggca gtaccgttat
    12121 cgtcgaccag ggcgaaaaac tctccctgaa agagacgtta accctgctgg atggtgccgc
    12181 acgtcataac gtacaggtcc tgataaccga cagcgggcag cgaaccggta caggcagtgc
    12241 actgatggcc atgaaggatg ccggggtgaa cacatatcgc tggcaggggg gagaacagcg
    12301 accggccacc atcatcagtg aaccggaccg taatgtccgc tatgcccggc tggccggaga
    12361 ttttgcggcc agcgtgaaag ccggagaaga gagcgtggca caggtcagcg gggtacggga
    12421 acaggccata ctgacagagg ccattcgcag tgagctgaaa acgcagggcg tgctcggaca
    12481 ccctgaggtg accatgactg cactgtcacc ggtctggctg gacagccgga gccgttatct
    12541 gcgggatatg taccgaccgg ggatggtgat ggagcagtgg aacccggaga cacgcagtca
    12601 tgaccgctat gtgatagacc gggtgacagc gcagagtcac agcctgaccc tgcgggatgc
    12661 gcagggcgaa acgcaggtgg tgcgtatttc ctccctggac agcagctggt cgctgttccg
    12721 gccggaaaaa atgccggtgg cagacggcga gcgactgagg gtgacaggga aaattcccgg
    12781 actccgcgtc tccggcggtg accgcctgca ggtggcatcc gtcagtgaag atgcgatgac
    12841 ggttgttgtg ccggggcggg ctgaaccggc caccctgcct gtggctgatt caccattcac
    12901 ggcactgaag ctggagaacg gctgggtgga aacgcccggg cattccgtca gcgacagtgc
    12961 gacggttttt gcctccgtca cacagatggc gatggacaac gcgaccctga acggtctggc
    13021 ccgcagtggt cgtgatgtcc ggctgtattc ctcgctggat gaaccccgta ctgcggaaaa
    13081 acttgcccgc catccctcct ttacggtggt ttctgatcag ataaaggcgc gtgccggtga
    13141 gacattgctg gaaaccgcta tcagtctgca gaaagccggg cttcacacgc cggcacagca
    13201 ggccattcat ctggcccttc ctgtgctgga aagtaaaaac ctggccttca gcatggtgga
    13261 cctgctgaca gaggcgaagt cgtttgctgc agaaggaacc ggttttactg aactgggagg
    13321 ggaaatcaat gcgcagataa aacgcggtga cttactgtat gtggatgtgg caaaaggcta
    13381 tggcacaggt ctgctggttt cccgtgcgtc gtatgaggca gaaaaaagta ttcttcgcca
    13441 tattctcgaa ggtaaggagg cggtcacgcc gttgatggag agagtacccg gcgaactcat
    13501 ggagacgtta acgtcgggac agcgtgccgc cacccgtatg atactggaaa cgtccgaccg
    13561 tttcacggtg gtacagggtt atgccggtgt gggtaagacc acacagttcc gggcggtgat
    13621 gtcagccgtg aacatgctgc cggcgagtga gcgtccccga gttgtggggc tggggcccac
    13681 gcaccgtgcg gtcggtgaga tgcgcagcgc cggcgtggat gcacagacgc tggcgtcctt
    13741 tctgcatgac acgcagctgc agcagcgcag cggagaaacg ccggatttca gcaacacgct
    13801 gttcctgctc gatgagagct caatggtggg caataccgat atggcacggg catacgccct
    13861 gattgcggcc ggtggcggtc gtgccgtggc cagtggtgac acggaccagc tgcaggccat
    13921 cgcgcccggt cagcctttcc gtctgcagca gacgcgcagt gctgccgatg tggtcatcat
    13981 gaaggagatt gtgcgtcaga cgccggaact gcgggaggcg gtatacagcc tgattaaccg
    14041 ggatgtggaa agggcactgt ccgggcttga gagtgtgaaa ccgtctcagg ttccccgtca
    14101 ggagggcgca tgggcaccgg agcactccgt gacggagttc agtcacagcc aggaagcgaa
    14161 actggcagaa gcgcagcaga aggcgatgct gaaaggcgag gctttcccgg atatccccat
    14221 gacactgtat gaggccattg tccgcgacta taccggcagg acaccggaag cacgggagca
    14281 gacgctgatt gtcacgcacc tgaatgagga ccggcgcgta ctgaacagca tgattcatga
    14341 tgcacgggaa aaggccggtg agctgggcaa agagcaggtc atggtgcctg tcctgaacac
    14401 agcgaatata cgtgacggag aactgcgtcg tctctccacc tgggagaata acccggatgc
    14461 ccttgccctg gtggatagtg tgtatcaccg gattgccggt atcagcaagg atgacgggct
    14521 gataaccctg gaggatgcgg aaggtaacac gcggctgatt tcgccccggg aggcggtggc
    14581 tgaaggtgtc acactgtaca caccagacaa aatccgggtg ggaaccggtg accggatgcg
    14641 cttcacgaag agtgaccggg agcgcggtta tgtggccaac agcgtctgga cggtgacagc
    14701 ggtttccggt gacagtgtca cgctgtcgga cggacagcag acccgggtga ttcgccccgg
    14761 tcaggagcgg gcagagcaac atattgacct ggcctatgcc atcaccgccc acggtgcgca
    14821 ggggacaagt gaaacctttg ccattgcgct tgaaggcacg gaaggtaacc ggaaactgat
    14881 ggcaggcttt gagtcagcct acgtggccct gtcgcgtatg aagcagcatg tgcaggtgta
    14941 caccgataac cgtcagggct ggacggatgc cattaacaat gccgtacaga aaggaacagc
    15001 ccacgatgtg tttgagccga aaccggaccg ggaggtcatg aatgcgcagc ggctgttcag
    15061 tacggcacgg gaactgcggg acgtggcggc aggccgtgcc gttctccgtc aggcggggct
    15121 ggccggggga gacagccctg cacggtttat tgcaccggga cgtaaatatc cgcagccgta
    15181 tgtggcactg ccggcgtttg accgtaacgg caggtccgcc ggtatctggc tgaacccact
    15241 gaccacggat gacggaaacg ggctgcgggg attcagcggt gaaggacggg tgaaaggccg
    15301 cggggatgcg cagttcgtgg ccctgcaggg cagccgtaac ggagagagcc tgctggctga
    15361 taatatgcag gatggtgtcc ggattgcccg tgataatccc gacagcggtg tggtggtaag
    15421 aattgccggt gaaggtcgtc cgtggaatcc cggtgccatc accggtggtc gcgtgtgggg
    15481 ggatatcccg gacagcagcg tccagccggg ggccggaaat ggcgaaccgg tcacggcaga
    15541 ggtgctggca cagcggcagg ctgaagaggc catccgccgt gaaacggaac gtcgcgcaga
    15601 tgaaattgtc cgtaaaatgg cagagaacaa acctgacctg ccggacggca aaacagagct
    15661 ggctgtcagg gatattgccg ggcaggaacg tgaccggact gccagttctg aacgggaaac
    15721 cgcactgccg gagagtgtgc tgcgtgaatc acaacgggaa cgggaagcgg tccgggaggt
    15781 tgcccgggaa aatctgctgc aggagcgtct gcagcagatg gagcgggata tggttcgtga
    15841 cctgcagaaa gagaaaaccc tgggcggaga ctgatacagg aagacaaaca ctgatgacaa
    15901 cggataacac gaacacgaca cgtaacgatt cactggctgc acggaccgat acctggttgc
    15961 agtcatttct ggtctggtca cccggacagc gggatatcat caaaacggtg gcactggtgc
    16021 tgatggtact ggaccacatc aatctgatat tccagctgaa gcaggaatgg atgtttctgg
    16081 ccgggcgagg agcctttccg ctgtttgccc tggtgtgggg gctgaatctg tcccgtcatg
    16141 cgcatatccg gcaaccggcc attaaccggc tgtggggatg gggaattatt gcccagttcg
    16201 catattatct ggccggcttt ccctggtatg aggggaatat cctgtttgcc tttgcagtgg
    16261 cggcacaggt gctgacgtgg tgtgaaacgc gcagtgggtg gcgtacagca gcggccattc
    16321 ttctgatggc cctgtggggg cctttgtccg gcaccagtta cggcattgcc gggctgctga
    16381 tgctggcagt cagccaccgg ctgtaccggg cggaagacag agcggaacgt ctggcgctgg
    16441 tcgcctgcct gctggctgtt attccggcgc ttaaccttgc caccagtgat gcagcggcgg
    16501 tggccggtct tgtgatgacg gtgctgaccg ttggtctggt gtcgtgtgca ggaaaatcat
    16561 taccccgttt ctggcccggg gattttttcc cggtattcta cgcctgtcat ctggctgtgc
    16621 tgggcgttct ggcgctgtga cgggtgtggt atctttggcc gcaagaggat gattcgtcag
    16681 aggcagaaca cagcatgaca gagcagaagc gaccggtact gacactgaag cggaaaacgg
    16741 aaggggagac gcctgtccgc agccggaaaa ccatcatcaa tgtcaccacg ccaccaaagt
    16801 ggaaggtgaa aaagcagaag ctggccgaga aagcagcccg ggaagcagag ctggcggcaa
    16861 aaaaagcgca ggccagacag gcgctgtcca tttatctgaa cctgcccaca ctggatgagg
    16921 ctgtgaacac cctgaagccc tggtggccgg gattatttga cggtgacacg ccccggcttc
    16981 tggcctgcgg tatccgggac gtgttactgg aagacgtggc gcagcggaat atcccgctct
    17041 cgcataaaaa actgcgcagg gcgctgaagg ccatcacccg ttcagaaagc tatctgtgtg
    17101 ccatgaaagc cggtgcctgc cggtatgata cggaagggta tgtgacggag catatttctc
    17161 aggaggagga agcgtatgcg gcagcgcgtc tggataaaat ccgccgccag aaccggataa
    17221 aggcagaact tcaggccgtg cttgatgaga aataaaaaaa gcctccctca gggaggggag
    17281 gcagggaaat aaggcttaaa aggaaataac ttccgtaccc gaaagaaagc cattccactg
    17341 agctatttat cacaataata ctgacaacta aagggggaca gaaatatgaa tggattcaga
    17401 aacagttcac ggaacggtca ggtctggtgc tggcagcgtg ccggaagccg ggccgttatt
    17461 ctggaggtca gtggacgcag gatggaagcg gcagaagcat ggcgacgggc tgccggtgtt
    17521 gcgccccgga cagactggca gcagtttgcc cgaaaaaggg ctgcacactg ccaccggcgt
    17581 tgccggggaa gggggtaacg aaaaaagcag ccttcattac tccaggagtc agaaggctga
    17641 aagaagttta tgagatttgc tttttttaat atccggtcgg gaagtatgac cggacagttc
    17701 tgagtagcac agattttttt ccgtattaag tcgagatttt atattttgtg attaatttca
    17761 caaaataagg tgttgttcaa tgtgtgctgt aatattcagg atgtctgaaa tacaggtgcc
    17821 atttatttga tatggagagt aacatgagga aatatattcc acgggtatta tttatctttt
    17881 catggcggta ttaacttccc tagtttcatc ttcatacctt attgggtgta ttaaaaacag
    17941 taggctgcca aagtcttaaa caagtttcaa ttctccatga cagttcaaca tcagccagag
    18001 gaaattccca ttctggacca gcgggagcat acgaacaata attttcggct tcgcgctata
    18061 gcgggctcaa gttaggttgt cccctgaatc tccagacaac taatatctga tcgcgccagt
    18121 ggtggctgtt attcaggatg gtatctcagt gcatgtcgat gtggttatcg ttgaacggcg
    18181 ttcgttcagt attgttgcag cagtatagct ggctgatgac agtcatttac gttcggaacg
    18241 cagatgctgg gatactcttc tggaagaggt tctgaggcag gctggcattc cgttgctcag
    18301 aagtcacgat gcaagaaaac tgctgcagat gaccgaagaa tggctgaata agacgcaggt
    18361 ttcagaaatg cgaagacccc actcgttaat ccattaactc gtgaggtctg catgatgcac
    18421 tttagcacta ctgattgtag cctcttcttt gcaaagatgt caaagtttat gacgaaatat
    18481 acccttatcg ggttgcttgc cgtgtgcgcc acggtgttgt gtttttcact gatattcagg
    18541 gaacagttat gtgaactgaa tattcacagg ggaaatacag tggtgcaggt aactctggcc
    18601 tgaagcacgg aagtaagctg ccgggcgggg acggaagtcc ccgctttccg gaagtgtgag
    18661 gtatttcagg ggcagacacc cgaaatgcta gaaactgccg gtcccgcccg gggccggtac
    18721 cctggttaaa gcatttccta cttttcagtc attttattat caaaattaca ttaaacggtc
    18781 gtaatcagac atgatttgtg cgccaacaca gatcatcgtc acaactttca agtcgctgat
    18841 ttcaaaaaac tgtagtattc tctgcgaaac gatccctgtt taagtattga ggaggcgaga
    18901 tgtcgcagac agaaaataca gtgacttcct catctggcgc aaaacgagca tacagaaagg
    18961 ggaatccgct ttctgatgca gagaaacaaa gattatcagt ggcccgtaaa agagcttcgt
    19021 tcaaggaagt aaaagtattt ctcgaaccaa agtataaggc catgctcatg caaatgtgta
    19081 atgaagatgg cctgacccag gctgaagttc tgaccgcact gataaaaagt gaagcgcaaa
    19141 aacgatgcat gtgatgatgg gcttacattc tgagtgttca gaagattagt gctagattac
    19201 tgatcgttta aggaattttg tggctggcca cgccgtaagg tggcagggaa ctggttctga
    19261 tgtggatcta caggagccag aaaagcaaaa accccgataa tcttcaccag gtttggcgac
    19321 taagagaaga ttaccggggc taacaagaaa ctgcatagaa gctgttgctc tatgcgggga
    19381 gtatagttat atgaccggaa aagttcaaga cttctttctg tgctcactcc ttctgtgcaa
    19441 cataagtgca ggatggtgtg actgatcttc accaaacgta ttaccgccag gtaaagaacc
    19501 cgaatccggt gttcacaccc cgtgaaggtg ccggaacgtt gaagttctgc gaaaaactga
    19561 tggaaaaagc ggtgggcttc agctcccgtt ttgatttcgc tattcatgtg gcgcatgccc
    19621 gttcgcgtgg tctgcgtcga cgcatgccac cggtgctgcg tcgacgggct attgatgcgc
    19681 tgctgcaggg gctgtgtttc cattatgacc cgctggccaa ccgcgtccag tgttccatca
    19741 ccacgctggc cattgagtgc ggactggcga cagagtccgg tgcaggaaaa ttctccatca
    19801 cccgggccac ccgggccctg acgttcctgt cagagctggg actgattact taccagacgg
    19861 aatatgaccc gcttatcggg tgctacattc cgaccgatat cacgttcaca ctggcgctgt
    19921 ttgccgccct tgatgtgtct gaggatgcag tggcagctgc gcgccgcagc cgtgtggtat
    19981 gggaaaacaa acagcgcaaa aagcaggggc tggataccct gggtatggat gaactgatag
    20041 cgaaagcctg gcgttttgtg cgtgagcgtt tccgcagtta ccaaacagag cttaagtccc
    20101 ggggaataaa gcgtgcccgt gcgcgtcgtg atgcgaacag gaaacgtcag gatatcgtca
    20161 ccctggtgaa acgacagctg acgcgtgaaa tctcggaagg gcgcttcact gccaatcgtg
    20221 aggcggtaaa acgcgaagtg gagcgtcgtg tgaaggagcg catgattctg tcacgtaacc
    20281 gcaattacag ccgcctggcc acagcttccc cctgaaagtg acctcctctg aataattcgg
    20341 cacgcgccgg aggcattcgc acgcctgaag cccgccagtg cacaaaaaat cagcaccaca
    20401 tacaaaaaaa caacctcatc atccaccttc aggtgcatcc ggtccccctt gtttttgata
    20461 caaaacacgc ctcacagacg ggtaattttg cttatccaca ttaaactgca agggacttcc
    20521 ccataaggtt acaatcgtta atgtcataaa gcgccagccg tcagccctac agggtgcaat
    20581 gtatctttta aacacctgtt tatatctcct ttaaactact taattacatt catttaaaaa
    20641 gaaaacctat tcactgcttg tcctatggac agacagatat gcacctccca ccgtaaacgg
    20701 cgggccccaa ccggagccac tttagttaca acacacaaaa ccaacctcca gaaaaacccc
    20761 gaaccagcgc agaactgaaa ccacaaagcc cctccctcat aactgaaaag cggccccgcc
    20821 ccggcccttc gggccggaac agagtcgctt ttaattatga atgttgtaac tacatcttca
    20881 tcgctgtcag tcttctcgct ggaagttctc agtacacgct cgtaagcggc cctcacggcc
    20941 cgctaacgcg gagatacgcc ccgacttcgg gtaaaccctc gtcgggacca ctccgaccgc
    21001 gcacagaagc tctctcatgg ctgaaagcgg gtatggtctg gcagggctgg ggatgggtaa
    21061 ggtgaaatct atcaatcagt accggcttac gccgggcttc ggcggtttta ctcctgtatc
    21121 atatgaaaca acagagtgcc gccttccatg ccgctgatgc ggcatatcct ggtaacgata
    21181 tctgaattgt tatacatgtg tatatacgtg gtaatgacaa aaataggaca agttaaaaat
    21241 ttacaggcga tgcaatgatt caaacacgta atcaatatct gcagtttatg ctggttatgc
    21301 tggctgcatg gggcattagt tggggagcca gatttgtcat ggagcaggcc gttctgcttt
    21361 atggatcagg aaaaaactat ttgttcttca gtcatggtac tgttctgatg tacctgctgt
    21421 gtgttttcct ggtataccgc cgttggatag ctccgctacc ggtcgttggt cagctgcgca
    21481 acgttggcgt accgtggctg gtcggtgcga tggccgtggt gtatgtcggt gtatttctgc
    21541 tcggtaaggc gctggctctg cctgctgagc catttatgac gaaacttttt gccgataagt
    21601 ccatacctga cgtgatcctg acgttgctga ccatctttat ccttgccccg ttgaatgagg
    21661 aaacgctgtt ccgggggatt atgctgaacg tcttccgttc acggtactgc tggacgatgt
    21721 ggctgggggc gctgataacg tcgttgttgt tcgtcgccgc gcacagccag tatcagaacc
    21781 tgctgacact ggcagaactg ttcctggtgg ggttgattac atcagtggcc aggatcagaa
    21841 gtggtggcct gctgctgccg gtattgctgc atatggaagc aaccacgctg ggtttactgt
    21901 ttggttgaaa gttatatttt tattaaacat tgtgcgttaa agcctggtgt gtttttttag
    21961 tggatgttat atttaaatat aacttttatg gaggtgaaga atgcatacca cccgactgaa
    22021 gagggttggc ggctcagtta tgctgaccgt cccaccggca ctgctgaatg cgctgtctct
    22081 gggcacagat aatgaagttg gcatggtcat tgataatggc cggctgattg ttgagccgta
    22141 cagacgcccg caatattcac tggctgagct actggcacag tgtgatccga atgctgaaat
    22201 atcagctgaa gaacgagaat ggctggatgc accggcgact ggtcaggagg aaatctgaca
    22261 tggaaagagg ggaaatctgg cttgtctcgc ttgatcctac cgcaggtcat gagcagcagg
    22321 gaacgcggcc ggtgctgatt gtcacaccgg cggcctttaa tcgcgtgacc cgcctgcctg
    22381 ttgttgtgcc cgtaaccagc ggaggcaatt ttgcccgcac tgccggcttt gcggtgtcgt
    22441 tggatggtgt tggcatacgt accacaggtg ttgtacgttg cgatcaaccc cggacaattg
    22501 atatgaaagc acggggcgga aaacgactcg aacgggttcc ggagactatc atgaacgaag
    22561 ttcttggccg cctgtccact attctgactt gaacatgggg tttgaggggc aactggatga
    22621 aaacgtacga tttagggcac taaaaccgct gttgtcccac cattctggtg attcccaaac
    22681 gttatttggc taaaaagtag ttttgatgtg gtttattttc cgaattccaa gcgcagccct
    22741 actttcttgt gcgtatttgc gtgttttgcg cagttttgaa gttcctacat cagattgact
    22801 tcggatacaa attcaatgaa cattgatgca aatactgacc tatcagtttc ttaactaaat
    22861 actcgagata catctaatgg ctaatgaaaa cttagatatt gactccaaga cagcagaaga
    22921 attatacgag tggtatctgg atgataaatt aattataaat cgaagatacc aaagaaaatt
    22981 agtatggtcc ctcgaagaga aaacagcact gatttcatca atgactcagc aataccctat
    23041 ccctttgcta ttgtttgttt ccattgacaa caaaagagaa atccttgatg gaatgcaaag
    23101 actagaagct ttaatgagtt ttattgagca gcgattttca ttaaatggta aatactttaa
    23161 tttagatgcc attgcattaa caaaacaact aaaagacagt ggtaaacttc aacaaaaaga
    23221 gccagtgcta tcccgcgaaa gttctacatt aattgctcgc tatagatttg ctatatctga
    23281 atactcttca acagaggacc acattgacga agtgtttcga cgcataaatt ccaatggtaa
    23341 aattttatca aaacaagaac tacgaagtgc ggggtgcgtt agtaattttt cagagttagt
    23401 aaggaaaata tcgacaatta taagaggaga tactacacat agcgatataa tgggactgaa
    23461 taaaatacat aacatatcaa tttgcaatga tggactcgat tatggaataa atatagacaa
    23521 tcatttctat attagaaatc atatcattag taggcctagc attagagact ctgacgatga
    23581 agagttagtt gcaaatatct tgggctatat atttttagat gataagccaa catcaggatc
    23641 aacatcgctc gatacatttt acggtgaagg ttcgacgagt catgcaatcc atacacgaac
    23701 tcaattagag aattacattc agacaaatgg tgcagataaa attgtcaata attacctctt
    23761 cgtttatgag atgattcaaa aattatttga tgcaaataat ttgaacttta gatcacacat
    23821 tctgggcaat gcatcttcat ctcaagaatg tcctcgttac tatcaagctg tttttcttgc
    23881 tttgtatgaa ttaatcataa atgaaaacat gcaacttgac gatgaacaga aatttatcgc
    23941 tcagttagga gacagtgtac aacgttcaat ggtgcaaacc gaagggggcc gttgggcagc
    24001 tagcgctcgt cagaaaagtg tcgaagattt atgtgcttta atcagacgat attttaaaga
    24061 atcagaaaat aaatttatca atcatgcttg gcaaacactg ataagaactc tacttaataa
    24121 ctcaagaaca gaacaaccaa attatgattt taaacagg
//
LOCUS       DAPOZO010000074        24091 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPOZO010000074 DAPOZO010000000
VERSION     DAPOZO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.074"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..643)
                     /gene="gloC"
                     /locus_tag="ROI21_003235"
     CDS             complement(<1..643)
                     /gene="gloC"
                     /locus_tag="ROI21_003235"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDX5112123.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPV"
     gene            complement(670..1218)
                     /locus_tag="ROI21_003236"
     CDS             complement(670..1218)
                     /locus_tag="ROI21_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDX5112124.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1399..3246)
                     /gene="ldtD"
                     /locus_tag="ROI21_003237"
     CDS             complement(1399..3246)
                     /gene="ldtD"
                     /locus_tag="ROI21_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDX5112125.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQSMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTAPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDTVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3507..7967)
                     /gene="mukB"
                     /locus_tag="ROI21_003238"
     CDS             complement(3507..7967)
                     /gene="mukB"
                     /locus_tag="ROI21_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDX5112126.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVLPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIVARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QSVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(7967..8671)
                     /gene="mukE"
                     /locus_tag="ROI21_003239"
     CDS             complement(7967..8671)
                     /gene="mukE"
                     /locus_tag="ROI21_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDX5112127.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(8652..9974)
                     /gene="mukF"
                     /locus_tag="ROI21_003240"
     CDS             complement(8652..9974)
                     /gene="mukF"
                     /locus_tag="ROI21_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDX5112128.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(9971..10756)
                     /gene="cmoM"
                     /locus_tag="ROI21_003241"
     CDS             complement(9971..10756)
                     /gene="cmoM"
                     /locus_tag="ROI21_003241"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDX5112129.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            10892..11671
                     /gene="elyC"
                     /locus_tag="ROI21_003242"
     CDS             10892..11671
                     /gene="elyC"
                     /locus_tag="ROI21_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDX5112130.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIASPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            complement(11648..12541)
                     /locus_tag="ROI21_003243"
     CDS             complement(11648..12541)
                     /locus_tag="ROI21_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDX5112131.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12695..13441)
                     /gene="kdsB"
                     /locus_tag="ROI21_003244"
     CDS             complement(12695..13441)
                     /gene="kdsB"
                     /locus_tag="ROI21_003244"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDX5112132.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKLMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQDVPGTGVDTPEDLERVRAEMR"
     gene            complement(13438..13620)
                     /gene="ycaR"
                     /locus_tag="ROI21_003245"
     CDS             complement(13438..13620)
                     /gene="ycaR"
                     /locus_tag="ROI21_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDX5112133.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13672..14904)
                     /locus_tag="ROI21_003246"
     CDS             complement(13672..14904)
                     /locus_tag="ROI21_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDX5112134.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDDSLARGELMEYWAHEACFMLRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHSRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAVWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(14941..15927)
                     /gene="lpxK"
                     /locus_tag="ROI21_003247"
     CDS             complement(14941..15927)
                     /gene="lpxK"
                     /locus_tag="ROI21_003247"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDX5112135.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAIKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(15924..17672)
                     /gene="msbA"
                     /locus_tag="ROI21_003248"
     CDS             complement(15924..17672)
                     /gene="msbA"
                     /locus_tag="ROI21_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDX5112136.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17709..19973)
                     /locus_tag="ROI21_003249"
     CDS             complement(17709..19973)
                     /locus_tag="ROI21_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDX5112137.1"
                     /translation="MKITTVGVCIICGIFPLLVLPQLPGTLTLAFLTVFACVLAFIPV
                     KAVRYIALTLLFFLWGISAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQVTHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGSFDSQRYAIAQHQPL
                     TGRFLQASVIEPDCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAMWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSVAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKYRYQQQGYKWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20180..20464)
                     /gene="ihfB"
                     /locus_tag="ROI21_003250"
     CDS             complement(20180..20464)
                     /gene="ihfB"
                     /locus_tag="ROI21_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDX5112138.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20624..22297)
                     /gene="rpsA"
                     /locus_tag="ROI21_003251"
     CDS             complement(20624..22297)
                     /gene="rpsA"
                     /locus_tag="ROI21_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDX5112139.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22408..23091)
                     /gene="cmk"
                     /locus_tag="ROI21_003252"
     CDS             complement(22408..23091)
                     /gene="cmk"
                     /locus_tag="ROI21_003252"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDX5112140.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDVLVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23264..24028)
                     /gene="ycaL"
                     /locus_tag="ROI21_003253"
     CDS             complement(23264..24028)
                     /gene="ycaL"
                     /locus_tag="ROI21_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDX5112141.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATL
                     SDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         5748 a         6630 c         5918 g         5795 t
ORIGIN      
        1 agacgggcat ttcgtcttgc aggaagggat tatgcaggcg ctcataacca agtgtggata
       61 atggtccgtg acccggaata aatgtcacgt catcccccag tggcagcaac ttatctttga
      121 ttgaagaaat cagttgatta tgatcgccac gcgggaagtc actgcgccct actccgcctt
      181 tgaaaataac gtcgccagaa atcagcagct ttgcccgatc atcaaaaaac acgacatgac
      241 ccggcgtatg ccctgggcaa tgtaacacct gtaaagccac attccctatg ctgatggtat
      301 cgccttcgtt cagccaacga tccggcgtca gcggctggca ctcttccaga ccaaacatac
      361 gactttgcgc aggcaagcct tgcagccaga actcatcttc tttttccggg ccaaaaaccg
      421 gcacgccgta atgttgcgcc agttccgccg ctgcaccaac gtggtccaga tgaccatgcg
      481 tcagcaggat ctgcatcagt gtcaggccgc tggcgtcaac ttcctgtttg attttttccg
      541 catcgccgcc aggatcgacc agtgcggcca gtcgggtttg ttcacaccag atcaatgaac
      601 agttctggga gaatgcggtg accggaataa tacgatagtt catactgccc ctgtttcgtt
      661 aagcaattac taccagtgcc gtgctggccc ggtatcaata tgcacaaagt tactacgtgg
      721 gtaatatcct acaccacctg cgcgcataga taacgcggct ttgcgaatat tgcttaacgc
      781 gataccttca atatggaaat ccatcgcctg ccctttcgtg tgatagcttt tcttcgctac
      841 tccacggctg cgggcgcgta gttcattgtt ggtatcaatg gagcgataac cggaaatgag
      901 ttgcaccggt ttgcgggtgc ctaacaaccc ttgcaggcga tacaactgat cgaataatcc
      961 tgggtcgatg gactttattt tgttcgcgcg ataatcgcgg aaaaaatggt taagttttgc
     1021 caattcttcc tgaatatagc ctctgccatc gaaaaactcc gctttgattg actctccggt
     1081 atgaagatta ttgagtgtca aaatgcgcgg gcgtggggta gagagtgttg caaacgcagg
     1141 ggtcggcagg atggcggcac cgagtgcaac gccaccaagc gccagcagtt tgcggcgatt
     1201 agcgtcgaat ttgtccatga taatcaagtc tacaggtcaa tgttatcgtt tatatgcact
     1261 tctggcgcac aagaggcacc ttaaccgtca gtaaagctga cgtcaagatg actcaacccc
     1321 agcagtgacg ggggctgcag agaatcgccc ccgatcatgc taattattac gacaactgat
     1381 ttccccgaac tgcttcattt acctgattaa ttgttccgct ttcgatacga tttgcgagct
     1441 ggatcgcgca ggcagatcat aattgtaaat atctgtacga tactgggtac gaccatctgc
     1501 accaacaaat gccgtcaggt agtagagatt taccggaatc gactgccgaa tattgacgta
     1561 acgcgtatca ccctgcttca gcgcatcaga aatacgtttg tcattccagc ctgcatcctg
     1621 caacagcata ttcgccagat cggaagcttt attcactcgt acacagcctg agctcaatgc
     1681 gcgtgtatca cgcttgaaca gattgtggtt cggcgtgtca tgcaaataaa tggcctctga
     1741 actcggcata ttgaatttat agcgccccag cgagttccgc gggcctggag cctgctggaa
     1801 gcgaaacggt aaatttgagg ccgtgattgt agaccagtca acctgccatg ggtcaatcgc
     1861 ttctctgctg ttccagccgc gcatcaccgt atagccatgg ctttcgagat atcccggatc
     1921 gttgcgcact ttcggcagaa tatctttgcg tgccagagtt ggtggtacgt tccacggcgg
     1981 gtttaccact acgttgttaa gtgcactact catcatcggc gttttgcgat cggggcgacc
     2041 gacaatgact cgcgaatcca gcacctgatt gccgttctga tagtagacca gcgaataggc
     2101 tggaatgtta accataatcc cggtagaaag ctctgttggc agcaagcgca atcgctggat
     2161 gttgagtgcc aacacgccag cacgctgggc gggcgttacg tttaaccagt cacgcgttgc
     2221 cgggccaata gcgccatctg ctcccaatcc ttgccatgcc tgaaaacgtt taacggcttc
     2281 caccagttca ttatcgtagg cggcacgaac ggcaggcgca ggtttactac gagacgtcgt
     2341 ttgcttgtcc atcggcttag tttctgctgt ttcaacagta acagcggatg ggctgactac
     2401 cgtgtcagtt ggcgtgtcat cgccaggtag agtaattttc ggccccccgt ccaacatgcc
     2461 tgtacgctgc aatatttcgc gcaacgccgg tacgtcgtta ctccactgcc ctgggcgcaa
     2521 cgttgctttg ccggtcagtt ggggccacgg tttggtgtca ctgagtaagg ccagtaacga
     2581 ttcatgcatc gctgcatatt gcggatgctg cggtgccaga cctgcaacaa acgtcggcaa
     2641 ttgaccttta tccagcgcca gttgccattg gttaatcacc gagagcggcg gcgttgaaag
     2701 cgcataaggt ttactgctat atagccagcg agtgcctttg accggaatat ttgcaatgaa
     2761 atggagatag cccatcatcg catcagagag cacgacgtcg cgtgccatcc cgttaacacc
     2821 aggagcggtc agtaactcta cccatttatt aaactgcggc tggaaaccgg caatcgccac
     2881 ctctgccagc tgttgctgga aggctttaac agcatcacgg ttttcccaca tggattgcat
     2941 atcgcgtgcg gcatacaaca gttgaagctg gttaagataa accggcttat aacctgcggg
     3001 taattgagat tcgatttgcg tgcgggcctt ttctgccgca ccttcaggca atggctggat
     3061 ccccgccatt attgccgtgg cttgcgcctg cggcagtgcc tcgccctgtt cactgacagc
     3121 caccgggctg tcgccaggga ttacttcagg ctcatcggcc tgcgcattga acagtggagc
     3181 gaatgttacg gccaggcaca aactgattgc cgacagccga cgaccacaca tcatattaag
     3241 caacatccct tgccccctgt ttttatcata ctctgaatag ccaggctgtt attttacgac
     3301 ttgcctgtac aacgccaata ctcacgaaag tatgacgagc aagcctgtta ctcatcaggc
     3361 ttatcttcag tatataaacg ccgatacgtt tttacataac ctaatgtaaa gaagtttaaa
     3421 gaaaagcgcg ccgtaatgtg ccactttttt agtgcagaaa cggagttttc ggaaaaagaa
     3481 aaggcggcat tgctgccgcc ttcattttaa ctcgcctggg aaggcgcttc gtcacttcct
     3541 ggaagcgttt cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacgtgttcg
     3601 gtattctgga agactttacg caccagttta taggtggtgc ctttttccgg actgatattt
     3661 tccggcgctg cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg
     3721 atagaacgag catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata
     3781 tctttaccgc gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc
     3841 gacataccgg taccaatcgc ctcaccggtc gacagagcgc cagattctgc gcgcagccag
     3901 ccatcagaac cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt
     3961 tcttcgccaa tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga
     4021 tacagtttcg ccagcgcttc tgagaaggtc agacggttgc tgttaaacag atcctgatgt
     4081 tgctcatgct gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg
     4141 ttgagacgca cgctgttcac ctggccaaac gatacgctct gcaagccctg gttgagcata
     4201 cggatacggt tctgctcgcg ctgaatggtt ttgcgaataa tgttcgccac gctgcgggaa
     4261 ctgatcgcca gtttctgttc acgggaggtt aactcttcgg tcagacggct aagttcgatc
     4321 tccatctgtt cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt
     4381 tcacgcagat gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga
     4441 tcttccgaca tccgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc
     4501 gcacccaatg ccttatccga catggaacgc aaatcatcag cggagagata agccagctca
     4561 cgacggtgta agcggcgttc aacgccgtta tctttcacca tgcgcatgac tgcacaccag
     4621 cccgctttcg cggtcactac ctgctcgcgc atctcaaagt aatcacgctc cagcttgcgc
     4681 agtttgcggg tcaggttgtc catctccgct tcgcagaagg tgagcgcttt ttccagttga
     4741 ttgcggcgcg aacggttatt gctcagttgc gcgtgcagct cgtcacggcg aatacgcgcc
     4801 cgctcttctg ccccgctatc agctcgcacg ccgatatcct gcaattcacg ttgcagatca
     4861 ttgagtagct cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac
     4921 tgactcaact gcgctgcgtg tccgcgcaac gcttcacgcg cgcgggtacg ctccgcttcc
     4981 gcctgttcca gacgttcacg cagtttttcg ttgagatcgc tgttaccgct cagcatttct
     5041 gccgagtcag agtagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgcc
     5101 tgctgacggg catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg
     5161 aactgttccg ggtcactctg caataccgaa acgatcggtt ccagtttcgc cagttgatta
     5221 ccgaactgct gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca
     5281 tcgacgcgat ctgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc
     5341 agcgccgtaa cgccctcttt cgcctgctca aattgaatac gctgctgctg gttatcattt
     5401 tcatgattac tcagcgcccg ctccagttcg acgcgacggc tgttcagttg acggatttct
     5461 gcttccggat cagactcaaa cgccaccgcc agatgactac cgataaagcg gctgaacgcc
     5521 tgatgcagac gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc
     5581 acttcacgct cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc
     5641 ggcacttccg ggaaacgtga ataacgccac tgacgatcgg cgattttcac cactaccgct
     5701 ttttccagct catcaacgct gaacacgctg tcatcgaatg actgcggatc cccttcgatc
     5761 agatagagat cttccgggca atcggtcaag ccttccaggt gctcggttac ctgtgacaga
     5821 tctggcacca cgatggcgtg gcgtgacggg ccatacagcg ctgagaagta cggcgcatct
     5881 tccaggctaa cgtcatcata aatttctgat agcagcacac caccaaaacg ctccgccagc
     5941 gcgttcagac gctgatcttc agagccgcct ggctggctta aacgttcgat ctcttcatcg
     6001 acggcgtttt tgcgtgcgcc aacttcgtca cgttcaacaa tcgcttcgcg ctcacgctcc
     6061 agcaactgtt gcagatattc ggtgacatcc tggctggaag agaactcttc gccgcactgt
     6121 tcgctcaact ggttgaggct gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa
     6181 ctctgaatgc gagactgcag ctgttcctgt tcctggcgca gtgccatgcg ctcttcacgg
     6241 gcgttagaca cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct
     6301 tccagttcat caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcaggcgt
     6361 tcggcttctt gctgctcgcg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggt
     6421 tgaacctgct ctgccaggtg acgctgatcg accccttcgc gcaatagttc gcgagcgaca
     6481 tcccacgcct cgttacgcgc cagcgggcca ttaattgcca ccaccagctg ataagcctgc
     6541 tcaaactggc tgtgtgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt
     6601 tcagtcgctt ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg
     6661 gttaagtccg gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta
     6721 tactggatcg cgcgcgtttg ctggacgtcc agcgcctgct gatagtcagc aagctggctt
     6781 ttcagctcgt ccacttccag ttcggcagct tccgcacgag cctcgttctc ttcctggcgt
     6841 tcgatggctt ctgccaccac ttcgttttgc tcttccagac ggatctgcag ctcatcgaga
     6901 tccgcttcgt aacgttcgat cttctcctgc tgacgcagtg cggtttgcac caggttcagg
     6961 tgatcgctgg ccgcctgata atccgcttcc agatcgcctt cggcaccgtt gtgctctgcc
     7021 agctcacgcg ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagtg
     7081 tgtagctcgc gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga
     7141 cgcatgtagt ccgccgccac gtagttggtg gcttcgctga tcagatgctt aaacaggtca
     7201 cggtccgact gggtgacacg aatcgcttcc agcgtcatgc ggttttcgcg cagcgccgct
     7261 tccatgtcct ggaacgcttt ccgcacgccg ctgttttccg gcaacaggta gtcgcgcaga
     7321 gagcgcgtaa tggcgctgga gatcccgccg tacagcgaag cttcgatcag acgatagaat
     7381 ttgctacggt cagacgctga gcgcagacga cgcgcgacga tgcccagatc gaacatcagc
     7441 gagtggtaat cggtaatgga gttgaactgc ttaaactgca cgccttccat cgcctcgagc
     7501 ttgtctttca gctcgttcag cggcagtaca cgcgcctggc gttcgttgag ggtttcggtc
     7561 accagctgcg tcggcagcac cgacatcggc aggccctgaa tggcaaacgg tttgatatcg
     7621 actttacgat cgcgtccggc aacctgttgc agacgcacac cgaccaccac acgctggtgg
     7681 cgcgagttaa tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga
     7741 cctttatcgc gcgaaccgct ggtggccccg gcttccgtgg tgttacggaa atgcagcagg
     7801 gtcaggtcgg ggatcagcgc cgtaacgaac gccgccatgg tggtggattt acccgccccg
     7861 ttaccgccgg agagcgtcgt aaccagctca tcgaggtcaa aagttcgggc aaaaaagccg
     7921 ttccagttaa tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc
     7981 cgctatctgg ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca
     8041 ttgcttcgcc atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca
     8101 catcggctcc gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca
     8161 taaaccacac catgcccaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca
     8221 acttctgccg gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg
     8281 cttcatcggc cagggtgagc agttcgtcgt acagttcctg ttgagtaaaa atcccctcat
     8341 tcgccagccg ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca
     8401 gttccgacaa gacagaacga gggatcagcg tggtggaacg tgggcgtaaa tagaagaacc
     8461 cttccggtgc acgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct
     8521 gaaaatccat caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac
     8581 gtaaggcgct atccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg
     8641 gcatcacttg ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta
     8701 atcggctgcc atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc
     8761 gcctgatcaa taacaatgcg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga
     8821 tattcgcgta ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcgagt
     8881 tgttcttcga tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga
     8941 tcctcaggaa gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata
     9001 tccagcagac gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt
     9061 accgactgac gtaaccgctg agcaaagaca cggtttttat ccatatcgat agcggtacgg
     9121 ataaatttat gtacgtggcg gtcgtagcca atccacaaat cgatggattg ctggccccag
     9181 ctgataatac ggtcaagttt gctctgcaga tcgaatacca gacgatcgac aaaatgcaga
     9241 tcgtcatggg tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct
     9301 gccgcttcca gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat
     9361 tcacagctgg agatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc
     9421 acctgctgct gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct
     9481 gctaccgaat atttcagtgg cgcataaaca ttacggtgcc agtgaaattc atcaccgccc
     9541 tcttcggcgg catctgccgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa
     9601 aggcgcagcg tagaaaactc gcgctgacgg atgtaatagt cagtaatgcc gatgccgagc
     9661 ggcgtcagac ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga
     9721 cgttgacgca ccatgtcgtt gatcgcgttg ttggcgcgca cgccgatggt ttcgctggtt
     9781 tgctcaaacg catcactcac atggcggaat gcatccacca gctcgccttc actcatctca
     9841 ccatccagac gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc
     9901 ggcagcgaga tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg
     9961 gaaaattcac tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac
    10021 gccccagggt aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt
    10081 catagcagtc gcgctgctgg tgcttctcgc gcagataatc atgaaacacg cgaacgcctg
    10141 tcttacccat aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc
    10201 gtggataatc tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat
    10261 caaaattccc ggcgaccatg ttatgcatca gcaagccgtg cgcattgtag aacattaacg
    10321 acaacacacc gcctggacgc aacactgacc acagggtctg taatacgctg cggggatcgg
    10381 ccacccactc gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag
    10441 caacatcctg agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg
    10501 ccgcctgttt tgcgcggtcg atcatctgcg cagaaagatc gcataaaatg acctgatgcc
    10561 cgcgctcagc cattttgatt gcggtctgcc cttctccacc gccagcatcc agcacacgca
    10621 gtttttgcgg gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc
    10681 gaagctgccc tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa
    10741 aattgcgatc ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc
    10801 tattttgtca cagccacggc gagaatgaac ccgtctggtg taatatcgct ggcgatccct
    10861 tgttatatgg ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg
    10921 caatatgttg ctgccacttc cgctgatgtt gttgattatt ggcgctggcc tggcgctgct
    10981 gtggtttagc cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcgct
    11041 cttgttattg agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta
    11101 tccgacatgg aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac
    11161 ctggaacccg cagtgggcac caagctcgaa tttaatcaat aacagtctgc cccgcctgaa
    11221 tgaaggtatt cgcttatggc gcgaaaatcc gggatcaaaa ctgatcttca cgggaggcgt
    11281 agcaaaaacc aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg
    11341 cgtgccgcgc gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc
    11401 ggcagtgaaa caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct
    11461 gccgcgggcg atgatctttt ttcagcagga agggctaaat ccgctgccag ccccggcgaa
    11521 tcagttggcc atcgcctcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtttg
    11581 gttgatgcat agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctaaa
    11641 aggttcgtca ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac
    11701 taaatcgtcc ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc
    11761 gccagatgaa tgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg
    11821 ccagagaatt atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc
    11881 ccggcccgac catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac
    11941 agaagttacc gtgaatcagc acacaattgt cgttaaagcc ttcaaataat gccgggagac
    12001 attcgcgagt acgaaacagg atccgcttat cctgcatcgt tagaccggtg ttattgaact
    12061 gattgagcgt ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt
    12121 cctgagtatt gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta
    12181 aggcttcaac gatttgatct ttgagttgtt cccagcgttc tggtgttcgg gctggtgcct
    12241 ccaccgaaac accacgcatc cgctccagta gcaggacatc ggggccgggg tgttcttcat
    12301 gcgtcatcac accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg
    12361 ttttccatgc cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca
    12421 ttgggtttcc ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc
    12481 actcaatacg actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca
    12541 ttcagattac cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca
    12601 cctgaatgag atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc
    12661 ctcccaaaat tggctggagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct
    12721 cgagatcttc aggggtatcc acacctgtgc caggaacgtc ctgggcgaca gcaacatgga
    12781 ttttttcgcc gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac
    12841 ttggctgcca gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat
    12901 gacgcaggaa gttatcgcca acggtttcaa ggccttttgc aaaacgatca cgatcccaag
    12961 gaatggtggc gcgagagaag tacagtgcat acccttcagc gtcgaggacc actttcaccg
    13021 cattcgggtt aaacgcttcc tccgcattgt ggattggcac cgccagagtc gccataccca
    13081 cctgacgctg ggcgagatta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt
    13141 catccccctg cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt
    13201 ccgccagacg ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag
    13261 cggcttcaac agcgcgggca acatcctcat gatcggttgc cacgatgatg cgctcggcac
    13321 ctgattcgcg cgcgcgttca agaacatgaa caatcatgag tttgccgtta atatcaacca
    13381 atggtttacc gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca
    13441 tgatttactc tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc
    13501 atcgcgcagc gggaaagcca ggttgtccag tttgcaaata agttcttgtt tttcctggtt
    13561 ataccaaagt tttccgttgc aaaccgggca ggcaatgatt tcaagcagac gatgatccat
    13621 agttcctcca gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac
    13681 ggggtctatt tcccagccgg tgcggcaatc agtaaagagg ccttgcgggc agcgtcctaa
    13741 tgtcacccgc gttgcctgct gccagttagc gaaatcagta atcgcctgac gtaacccttt
    13801 ttgcagcgtt gtcgttggtt taatgccttc ttgtaaccac agagagataa cttcaaggat
    13861 gcctgtctgg cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa
    13921 cggcagaaca aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta
    13981 gctaaaatca aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga
    14041 aagtaccgcg ctgtgcgttg cagtgagctt tccggccagt gcacgctcga gtagcggcag
    14101 caaatcagca tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg
    14161 ctgttcagcc ctcgcttcgc gccaaaccgc cagtgccgga cgtttcagcc gatagtaatc
    14221 tgccagccac tgttcgcgga atatgccaag gctacgcgca ctgttatcca gcatgatgat
    14281 ttctgcttct gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacgatg
    14341 ggttaaatca taaacgcgct ggaagttgcg ccgttcaatc accatcactt tcccggctgt
    14401 aaataaacct tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt
    14461 acgagaatgc tcgaaatcgg ctgaacgtac cggcccctta tcatgaatat gctgaattaa
    14521 ctgtgcaatt tccgccgcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt
    14581 ttcaggtgcc agcatgcggt ggcgaataag acgaaagtcg ctacgcagca taaagcaggc
    14641 ttcatgcgcc cagtattcca ttaattcacc acgcgccaga gaatcatcca gccactgcgc
    14701 aggataattt cccagacgac tgaaaagcac cagatatggg ctacgggcaa caatattgat
    14761 ggtatcgatt tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgatgc
    14821 tcgacggcgg ggtttgttta acaggccttg cgcggcaagg tgaagattac gcgcatcagc
    14881 aagggagagg tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa
    14941 ctagttgcca gaagccagcg aggttagttg cgcaagcagt ttcgctggtt catcacctga
    15001 aagctgtgcg tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac
    15061 cgcatctttt tcagtcatta ccagcgtttg cccggcgctt accaacgcgc tgacatccgc
    15121 atgattcaaa gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat
    15181 cttcagcgtg gcaaaaaagc gcggcggatg accaatccct gccatcgcca ccacatgttc
    15241 aagctgagca acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag
    15301 atgcatgggg atttcaccgc tgcgggggac accaccgttg acgattaccg catcaaccga
    15361 ctttaagcgc cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa
    15421 gcgacgcaca ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa
    15481 accgtcgtcg gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttatcgc
    15541 atcagaacgc acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg
    15601 ttcatcaccc gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc
    15661 cttaccacca tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc
    15721 caccagccag acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac
    15781 gacaaccggt acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat
    15841 cgcgccactc accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga
    15901 ttcaccagac cagatttttt cgatcattga ccaaactgca ttttgtgaag ttgcgcgtaa
    15961 acgccacggt gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg
    16021 acgaccacga tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag
    16081 gtacggtttt tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc
    16141 aacgccgagg tagcttcgtc cagaatcaga atcgggctat cacgcaacaa ggctcgagcg
    16201 atggcaatac gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta
    16261 tcgagaccgt tatccatctt attgatgaag tccatggcgt aggccatacg cgccgcttct
    16321 tcaatttgct cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaacagta
    16381 tcgttaaaca aatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg
    16441 gtatactcgc gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa
    16501 cgtgtgatta ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc
    16561 gtcttccctg ccggaatttt caggttgatg ttacgcaatg cgggcacgtc acgtcctgga
    16621 taagtaaagg tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta
    16681 ccttcgtctt tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata
    16741 ccgcgctgga actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc
    16801 attgaagaga aaacaacggt aatcgtaccg gcagtcaggc tatccatgac acttgggaag
    16861 ctcgccgcat acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca
    16921 gagatggaag aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta
    16981 tcgaagcgtt tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc
    17041 agcatttgtt cagcgctggt ggtcacctgt cccatggtgt tctgcatgtt tttactgatg
    17101 ttgcgaaaac gcttcgatac aacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc
    17161 aaaatgatcg acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac
    17221 gcaccttcac gcacaacagt aatcagtgcg ccggaagagg aagaagcaac ctgttcggaa
    17281 tcgtaggtga tacgtgacaa cagcgtaccc gttgactgtt tgtcaaagaa tgaaaccggc
    17341 attcccatca tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc
    17401 caggagatac agtagctgga gacatagctg gtgataccac gcaaaatcat cagcccgatc
    17461 accaccagcg gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga
    17521 agtggcttaa ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg
    17581 cccgccacga tcagacccgc tttaaaaggc gcaatggttg gccacagtcg gcggaatgtc
    17641 tgccacgtag agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc
    17701 cgcatattct acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa
    17761 atttgctctc tgaggctgct aatccgccag ccttgcgctg aaaaattgac cgtcacttga
    17821 ccctgatgtg gagtatcaag ccacttataa ccttgttgtt gatagcgata cttaactttg
    17881 ttggagggca atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc
    17941 actcgctgaa ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc
    18001 aatgttgcct gcacttgctg ccagtaacgg cttagcatct tttgttcagc gggggcttca
    18061 atatcaccgg ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg
    18121 ttatttcctt tatcgttgct agcttgtaaa ggccagtgcg cgctgaaacg caatccttgc
    18181 cattgccacg cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg
    18241 atccataaca tcggccatgt gtgcaatatt gagtccagcc ctccccggtg atccagatgt
    18301 tcatgactca gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc
    18361 agttgttgcc cactatcccc ttcgggccag gccagtcccg tgtcatagag aatcgctttg
    18421 ccgtttcgcg caatgaccat tgccagccct tgcccgacat caagcatgta cacttgccac
    18481 tcgtcaggtc gaggtttttg ccacagcggc cagcacatca gcaagcctcc agccacacac
    18541 attgctggca acgttcgcca ggcgtttaat cgccatacca ccagtaagaa ccatggggaa
    18601 aatgttagcc attgccaacg ttcagcaatg ttgatccacc cttccggcaa actctttaac
    18661 ccccagaaaa gtaaagccaa agaccggtcg gcaagaaacc ataacccctg ttccaggatt
    18721 aacggcccgc ttaaatgcac aaccatcgcg gcgaggatca atggaaccgt gataaatgtc
    18781 accaggggaa ttgcaaatag atttgcaata aacgaggtca gactaatgcc atgaaatatg
    18841 acgatttgca cgggcataag caggagggtg attcccagct gcagatggat gagggaaaca
    18901 accgcgcgca ataccggcgg cagttgccac tccggacagg gaaaccactg ataccaaaaa
    18961 atcagtgccg cgaccgcagc gacagagagc cataaacttt gcgagagaat ggcaacagga
    19021 tccatcagca aaattgccgc cagacaacat atccatacat cccagccact ccattgtcgc
    19081 ccacttaact taagcattcc ccacatggca agcgccacca cggtacgcaa tgcaggaggt
    19141 tgcatcccag tcagccaggc ataaaaagca gcacagcaga ttccgccaat taatggcatt
    19201 tgccagtgga tccagcgccc aggcagaaaa acttgcccac cgcgaatgag tccggcagcc
    19261 agcaacgccg caaaagcgat atgcaatccc gatatcgcca ttaaatgcgc cgttccagta
    19321 tcgcgcatga tatttttgat ttctttggga acggataacc gttcccccat acctaaacca
    19381 agaataactg cattccacgg atagggttgc agcgttgttt gtaatgacgc cagatactgt
    19441 gcacgcaagc tacaatccgg ttcaatgaca cttgcctgca gaaagcggcc ggtcagcggc
    19501 tgatgctggg caatggcata acgctggcta tcaaagctgc catcattaag ttggccatga
    19561 actgcacgaa ctttgagttt cattgaccat agttgtccgg cacaaaccgc ttgcgggaga
    19621 tattcgccat acagcacgag gcctggcgca gggaatatac gtcgaccttg tagatgagtg
    19681 acttgaccgt aatgagtggt catgccgtca gtagcagtga tctcaactat tgcatcctgc
    19741 gtcgcgccag ttaaggtttc tcctgcccac aaaatttgct ttgccgctga tatgccccaa
    19801 agaaaaaaca gcaacgtcag cgcgatataa cggacggctt taacaggaat aaatgccagt
    19861 acgcaggcga agacagtcag aaacgcaagg gttaatgtcc caggcaattg gggcaaaacc
    19921 agcaacggaa aaattccgca aattatgcat acgccgactg tcgttatttt catgcacccc
    19981 tccgtattta tggaaagtgt gcaactttgt tgagcggttg tctggtggga gttttgcgtt
    20041 ccccgaggca cgttcagaag aatttaacga gagggtgaaa actgttcaaa actctgcgag
    20101 gcagcttcca gttccagatt agagaaatga aaaaagcacc cgacaggtgc ttttctcgcg
    20161 ttcaagtttg agtaaaaact taaccgtaaa tattggcgcg atcgcgcagt tctttacctg
    20221 gtttaaagtg aggaacgtat tttccttcca gttctacttt atcgccagtc ttcggattac
    20281 gtccggtacg tggtgcgcgg tagtgcaaag agaaactgcc gaaaccgcgg atttcaatac
    20341 gctcgccctg cgcaagagtc gaggccatat gctccagcat ctcttttact gcatcttcaa
    20401 ccgtcttggc gggaatgtgc gattgctggg tggcaagtct ttctatcaat tctgacttgg
    20461 tcatgattcc tccggttcct taaaggcaaa ttaagcggct gctgttgctg cattgattaa
    20521 gggcggccgt agccgccctt agtgcttgat tacaggacga aacctgcaat ctgtcaagta
    20581 aactcaacaa acttcggaat aaaaatcccg aagagtcaga gaattactcg cctttagctg
    20641 ctttgaaagc ttcagccatt gcgttgttgg agaagtttgc atcttcctgt ttgttaacag
    20701 ttgcgattgc atctttctcg tcagcttcgt ctttcgcacg aacagacagg ctgattgcgc
    20761 ggtttttacg atcaacgccg gtgaatttag cttcaacttc gtcgccaacg ctcagaacca
    20821 gggtagcgtc ttcaacgcgg tcacgggatg cttcagaagc acgcaggtaa ccttcaacgc
    20881 cgtcagccag ttctacggtt gcgcctttag cgtcaactgc agttacttta ccggttacga
    20941 tagcgccttt cttgttcaga gcaacccagt tgttgaacgg atcttctgcg agctgtttaa
    21001 cgcccaggga gatacgttca cgttctgcgt caacctgcag aacaactgca gcgatttcgt
    21061 cgcctttttt gtattcacga actgcttctt cgcctgcaac gttccaggag atgtcagaca
    21121 ggtgaaccag gccgtcgatg ccgccgtcaa ggccgatgaa gataccgaag tcagtgatag
    21181 acttgatttt accttcaaca cggtcaccct tgttgtgggt ttccgcgaac tgctgccacg
    21241 ggttagcttt gcactgtttc agacccaggg agatacgacg acgttcttcg tcgatatcca
    21301 gaaccataac ttccactaca tcgccaacgt taacaacttt ggacgggtgg atgtttttgt
    21361 tggtccaatc catttcggaa acgtgtacca ggccttcaac gccttcttcg atttcaacga
    21421 agcagccgta gtcggtcagg ttggtcacgc gaccagtcag tttggtacct tccggataac
    21481 gtttagcgat agctacccac ggatcttcgc ccagctgttt caggcccagg gatacacggg
    21541 tacgttcgcg gtcgaacttc agcactttaa cagtgatttc gtcgcccacg ttgacgattt
    21601 cgctcggatg cttaacgcgt ttccaggcca tatcagtgat gtgcagcagg ccgtcaacgc
    21661 cgcccagatc aacgaatgca ccgtagtcag tgaggttctt aacgatacct ttaacttcca
    21721 tgccttcctg caggttttcc agcagctgat cgcgctctgc gctgttttcg gattcgataa
    21781 cggcacgacg agaaacaaca acgttgttgc gcttctgatc cagcttgatt actttgaatt
    21841 caagctcttt gccttccagg tgcagagtgt cacgcaccgg acgaacgtct accagagaac
    21901 ctggcaggaa cgcacgaata ccgttcagct caacagtgaa gccgccctta actttgccgt
    21961 tgataacacc ggtaacagtt tcagcatctt cgtaagcttt ttccagcgtg atccaggctt
    22021 cgtgacgttt agctttctca cgggacagca gagtttcacc gaagccgtct tctactgcgt
    22081 ccagagcaac gtcaacttcg tcacctacct ggatttccag ctcgccctgg gcgtttttga
    22141 actgctcagc cgggatggcg gactcagatt tcagaccagc gtcaaccagt actacgtctt
    22201 tgtcgatagc aacaacaacg ccacgaacga tagaacccgg gcgggtttcg atttctttta
    22261 aggactcttc aaagagttga gcaaaagatt cagtcatgtt taatcttcag gtttatattt
    22321 aacgtccacc tggctccatg ccggatgggg ttgtttaaca tacccgctgt cattccattg
    22381 caacgggggt actgcaaatt cggtcgctta tgcgagagcc aatttctggc gcgcgtattg
    22441 tagcgctttt tcaatcactt gctcaatgct taaggtggtg gaatccaaca ctaaagcatc
    22501 ggctgccgga accagtggcg ctaccgctcg gttacgatcg cggtcgtcgc gttctttgat
    22561 ctcggccaaa aggcgctcaa agttaacact aaagcccttc tcctgcaact gtagcatgcg
    22621 gcgatgcgca cgttcttccg aggaggcgtc aaggaaaatt ttcactggcg catcagggaa
    22681 taccaccgtt cccatgtcgc ggccatcggc aatcaagcct ggtaattcgc gaaacgcgcg
    22741 ttggcgacgc aataatgctt cacgaacgcg tgggaatgcc gcgacttgtg aagcagcatt
    22801 cgccacttcc tgagtacgaa tttcgccact gacatcttcc ccttcgagga tcacttccag
    22861 attgccattg gtcgacacaa aacgtacatc cagatgggat gccagcggca caagcacatc
    22921 ttccgatgca acatcaacat ggtgatgtaa tgccgccagt gccagtacgc gataaattgc
    22981 acccgagtcc agcagatgcc attgcaacgc ttccgccata gccttacaca aggtgccttt
    23041 ccctgcaccg cttgggccat caatggtaat aaccggggca attgccgtca tctttatctc
    23101 cttaaaacag gcgtaccgtt aacataaccg cgcgcattat acgcgccgac gccacaaatc
    23161 gttaacgttg tgtgcaaaaa ccagttttgt ctgtattcag atggggattt attgcgcaat
    23221 tatagcgggc tggcagaaga ccagcccgaa agatgacaat gatttactta ccagaggcga
    23281 tacgatcacg gatgtgttgc gcacgctctg ttgatggtgg gtgagagtca aacatggact
    23341 gggtacgacc gccatccagg ctggccaatt tttcaaagct gccaaccagc ccctgggtgc
    23401 tgatgccacg tttcttcaac agatcaaagg agaaatcatc tgcttcggac tctttatcgc
    23461 gggagtattt agcattgatc gcgccttctg cgatatcacc taattgtgag cgagaaagct
    23521 gggaagccac accgctggta gcggaaatcg catcacgtgc ggcaacgatc gcataagaag
    23581 ctttcatttc agccagcgag tgacccaacg cgacgtggcc cagttcatga cccagaacgc
    23641 cttcaatttc gttgtcgttc atcatgtcca tcaggccact gtagacacga acacagccgt
    23701 tcgccatcgc ccatgcgttg acgtcgctgg tcatataaac cttatagttg actggcgtgc
    23761 cattaatatt gttacccaat gctttggcga tttttgccag acgtttcccg tacttgctgg
    23821 atttgcttgc cactttattg ccgctgtcca tttcgacaca gccctgattg gcgattgctt
    23881 tagcatcagc atcgcttaat gttgcggctt tataagcatt cagacctgag ctgatagcca
    23941 aatcggtatt aataccgtgc gtattctgac atcctgtcag taaggctgca gaggtcgcaa
    24001 tcgccagcaa taatttagta ttcttcatgt gaatcatccg ttgattaatt atatataaac
    24061 attatgtatc aacaagttac attgataatt a
//
LOCUS       DAPOZO010000075        23718 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPOZO010000075 DAPOZO010000000
VERSION     DAPOZO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.075"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            23..2485
                     /gene="thrA"
                     /locus_tag="ROI21_003254"
     CDS             23..2485
                     /gene="thrA"
                     /locus_tag="ROI21_003254"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDX5112142.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            2487..3419
                     /gene="thrB"
                     /locus_tag="ROI21_003255"
     CDS             2487..3419
                     /gene="thrB"
                     /locus_tag="ROI21_003255"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDX5112143.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            3420..4706
                     /gene="thrC"
                     /locus_tag="ROI21_003256"
     CDS             3420..4706
                     /gene="thrC"
                     /locus_tag="ROI21_003256"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDX5112144.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFVFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAIAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            4920..5216
                     /locus_tag="ROI21_003257"
     CDS             4920..5216
                     /locus_tag="ROI21_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDX5112145.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHDKHNDHRGDHRPGPDKHHR"
     gene            complement(5256..6032)
                     /gene="yaaA"
                     /locus_tag="ROI21_003258"
     CDS             complement(5256..6032)
                     /gene="yaaA"
                     /locus_tag="ROI21_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDX5112146.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLSGINAARFHDWQPDFTPKNARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(6102..7532)
                     /locus_tag="ROI21_003259"
     CDS             complement(6102..7532)
                     /locus_tag="ROI21_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDX5112147.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVAQLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            7811..8764
                     /gene="tal"
                     /locus_tag="ROI21_003260"
     CDS             7811..8764
                     /gene="tal"
                     /locus_tag="ROI21_003260"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDX5112148.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            8879..9466
                     /gene="mog"
                     /locus_tag="ROI21_003261"
     CDS             8879..9466
                     /gene="mog"
                     /locus_tag="ROI21_003261"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDX5112149.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(9501..10067)
                     /gene="satP"
                     /locus_tag="ROI21_003262"
     CDS             complement(9501..10067)
                     /gene="satP"
                     /locus_tag="ROI21_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDX5112150.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(10216..10929)
                     /gene="msyB"
                     /locus_tag="ROI21_003263"
     CDS             complement(10216..10929)
                     /gene="msyB"
                     /locus_tag="ROI21_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDX5112151.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(10955..11359)
                     /locus_tag="ROI21_003264"
     CDS             complement(10955..11359)
                     /locus_tag="ROI21_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDX5112152.1"
                     /translation="MKSVFTLSASLAISLLLCCTAQANNHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLTADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            11734..13650
                     /gene="dnaK"
                     /locus_tag="ROI21_003265"
     CDS             11734..13650
                     /gene="dnaK"
                     /locus_tag="ROI21_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDX5112153.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            13739..14869
                     /gene="dnaJ"
                     /locus_tag="ROI21_003266"
     CDS             13739..14869
                     /gene="dnaJ"
                     /locus_tag="ROI21_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDX5112154.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(14973..15125)
                     /gene="mokC"
                     /locus_tag="ROI21_003267"
     CDS             complement(14973..15125)
                     /gene="mokC"
                     /locus_tag="ROI21_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDX5112155.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            15711..16877
                     /gene="nhaA"
                     /locus_tag="ROI21_003268"
     CDS             15711..16877
                     /gene="nhaA"
                     /locus_tag="ROI21_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDX5112156.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNHADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            16943..17842
                     /gene="nhaR"
                     /locus_tag="ROI21_003269"
     CDS             16943..17842
                     /gene="nhaR"
                     /locus_tag="ROI21_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDX5112157.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(17880..18839)
                     /locus_tag="ROI21_003270"
     CDS             complement(17880..18839)
                     /locus_tag="ROI21_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDX5112158.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCAVTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKLSYNLEEMLSAINFTIPYSIASVSDSSDTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNEIYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQDSGTSILKNDLENSEDGAGQIGFQLSNDGTNEMQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(18852..21302)
                     /locus_tag="ROI21_003271"
     CDS             complement(18852..21302)
                     /locus_tag="ROI21_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX5112159.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMRGKKVSEYRF
                     DNKQPLPGNYELDVYVNHQWRGKQDITIPESPAKPCLPKVLLTTLGVKTDNLNTEDNC
                     ILLDEAVHGGQYQWDISEHRLNLTIPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSNTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGTWQSNTLYLERGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSIALITVS
                     QNGYIIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     VSNFDFIAGRSKIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDITHEGTSYQVAYNKYLVQTATRFSVAAWRYASQDYRTFS
                     DHLYENDKHNHQSEYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNSWQRISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NSKGQVVYDLMIPHRENHLVLDIANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRTDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            complement(21315..21998)
                     /locus_tag="ROI21_003272"
     CDS             complement(21315..21998)
                     /locus_tag="ROI21_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDX5112160.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKSREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSNNDITLKNNNASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            complement(22048..22581)
                     /locus_tag="ROI21_003273"
     CDS             complement(22048..22581)
                     /locus_tag="ROI21_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5112161.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLSAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            complement(22882..>23718)
                     /gene="espX1"
                     /locus_tag="ROI21_003274"
     CDS             complement(22882..>23718)
                     /gene="espX1"
                     /locus_tag="ROI21_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HDX5112162.1"
                     /translation="LYNEQCLKIPASSTDDNQEITWRNFKTSNAAYSQLCEKSSLFKS
                     VPSRLIEKSAYCSTENMVTNKFGVVFSYCGDNIKEFILLLPYNKSLEMYELNEQKIQY
                     LITHNININKLLLSNITIEKSNLSYGYYFGCVLSNISCFESDLSNTIFSNGEINNLFI
                     KKSNIFGTSFTNTRIKNLCCEDIMPGRWNTQLVNKHLGYRYTGVFKTLASIDDKPSRF
                     EILIPLIQTLVRDNVKLNNDVYKELNNFMLDYDKTSSEMRKYLKSINECMFLMKNIVH
                     QD"
BASE COUNT         6123 a         5471 c         5891 g         6233 t
ORIGIN      
        1 caaaggtaac gaggtaacaa ccatgcgagt gttgaagttc ggcggtacat cagtggcaaa
       61 tgcagaacgt tttctgcgtg ttgccgatat tctggaaagc aatgccaggc aggggcaggt
      121 ggccaccgtc ctctctgccc ccgccaaaat caccaaccac ctggtggcga tgattgaaaa
      181 aaccattagc ggccaggatg ctttacccaa tatcagcgat gccgaacgta tttttgccga
      241 acttttgacg ggactcgccg ccgcccagcc ggggttcccg ctggcgcaat tgaaaacttt
      301 cgtcgatcag gaatttgccc aaataaaaca tgtcctgcat ggcattagtt tgttggggca
      361 gtgcccggat agcatcaacg ctgcgctgat ttgccgtggc gagaaaatgt cgatcgccat
      421 tatggccggc gtgttagaag cgcgtggtca caacgttacc gttatcgatc cggtcgaaaa
      481 actactggca gtggggcatt acctcgaatc taccgtcgat attgctgagt ccacccgccg
      541 tattgcggca agtcgtattc cggctgatca catggtgctg atggcaggct tcaccgccgg
      601 taatgaaaaa ggcgaactgg tggtacttgg acgcaacggt tccgactact ccgcggcggt
      661 gctggctgcc tgtttacgcg ccgattgttg cgagatttgg acggacgttg acggggtcta
      721 tacctgcgac ccgcgtcagg tgcccgatgc gaggttgttg aagtcgatgt cctaccagga
      781 agcgatggag ctttcctact tcggcgctaa agttcttcac ccccgcacca ttacccccat
      841 cgcccagttc cagatccctt gcctgattaa aaataccgga aatcctcaag caccaggtac
      901 gctcattggt gccagccgtg atgaagacga attaccggtc aagggtattt ccaatctgaa
      961 taacatggca atgttcagcg tttctggtcc agggatgaaa ggaatggtcg gcatggcggc
     1021 gcgcgtcttt gcagcgatgt cacgcgcccg tatttccgtg gtgctgatta cgcaatcatc
     1081 ttccgaatac agtatcagtt tctgcgttcc acaaagcgac tgtgtgcgag ctgaacgggc
     1141 aatgcaggaa gagttctacc tggaactgaa agaaggctta ctggagccgc tggcggtgac
     1201 ggaacggctg gccattatct cggtggtagg tgatggtatg cgcaccttgc gtgggatctc
     1261 ggcgaaattc tttgccgcgc tggcccgcgc caatatcaac attgtcgcca ttgctcaggg
     1321 atcttctgaa cgctcaatct ctgtcgtggt aaataacgat gatgcgacca ctggcgtgcg
     1381 cgttactcat cagatgctgt tcaataccga tcaggttatc gaagtgtttg tgattggcgt
     1441 cggtggcgtt ggcggtgcgc tgctggagca actgaagcgt cagcaaagct ggctgaagaa
     1501 taaacatatc gacttacgtg tctgtggtgt tgccaactcg aaggctctgc tcaccaatgt
     1561 acatggcctt aatctggaaa actggcagga agaactggcg caagccaaag agccgtttaa
     1621 tctcgggcgc ttaattcgcc tcgtgaaaga atatcatctg ttgaacccgg tcattgttga
     1681 ctgcacctcc agccaggcag tggcggatca atatgccgac ttcctgcgcg aaggtttcca
     1741 cgttgttacg ccgaacaaaa aggctaacac ctcgtcgatg gattactacc atctgttgcg
     1801 tcatgcggcg gaaaaatcgc ggcgtaaatt cctctatgac accaacgttg gggctggatt
     1861 accggttatt gagaacctgc aaaatctgct caatgcaggt gatgaattga tgaagttctc
     1921 cggcattctt tctggttcgc tttcttatat cttcggcaag ttagacgaag gcatgagttt
     1981 ctccgaggcg accacgctgg cgcgggaaat gggttatacc gaaccggacc cgcgagatga
     2041 tctttctggt atggatgtgg cgcgtaaact attgattctc gctcgtgaaa cgggacgtga
     2101 actggagctg gcggatattg aaattgaacc tgtgctgccc gcagagttta acgccgaggg
     2161 tgatgttgcc gcttttatgg cgaatctgtc acagctcgac gatctctttg ccgcgcgcgt
     2221 ggcgaaggcc cgtgatgaag gaaaagtttt gcgctatgtt ggcaatattg atgaagatgg
     2281 cgtctgccgc gtgaagattg ccgaagtgga tggtaatgat ccgctgttca aagtgaaaaa
     2341 tggcgaaaac gccctggcct tctatagcca ctattatcag ccgctgccgt tggtgctgcg
     2401 cggatatggt gcgggcaatg acgttacagc tgccggtgtt tttgccgatc tgctacgtac
     2461 cctctcatgg aagttaggag tctgacatgg ttaaagttta tgccccggct tccagtgcca
     2521 atatgagcgt cgggttcgat gtgctcgggg cggcggtgac acccgttgat ggtgcattgc
     2581 tcggagatgt agtcacggtt gaggcggcag agacattcag tctcaataac ctcggacgct
     2641 ttgccgataa gctgccgtca gaacctcggg aaaatatcgt ttatcagtgt tgggagcgtt
     2701 tttgccagga gctgggtaag caaattccag tggcgatgac cctggaaaag aatatgccga
     2761 tcggctcggg cttaggctcc agcgcctgct cagtggtcgc ggcgctgatg gcgatgaatg
     2821 aacactgcgg caagccgctt aatgacaccc gtttgctggc tttgatgggc gagctggaag
     2881 ggcgtatctc cggcagcatt cattacgaca acgtggcacc gtgttttctc ggtggtatgc
     2941 agttgatgat cgaagaaaac gacatcatca gccagcaagt gccagggttt gatgagtggc
     3001 tgtgggtgct ggcgtatccg gggattaaag tctcgacggc agaagccagg gctattttac
     3061 cggcgcagta tcgccgccag gattgcattg cgcacgggcg acatctggca ggctttattc
     3121 acgcctgcta ttcccgtcag cctgagcttg ccgcgaagct gatgaaagat gttatcgctg
     3181 aaccctaccg tgaacggtta ctgccaggct tccggcaggc gcggcaggcg gtcgcggaaa
     3241 tcggcgcggt agcgagcggt atctccggct ccggcccgac tttgttcgct ctgtgtgaca
     3301 agccggatac cgcccagcgc gttgccgact ggttgggtaa gaactacctg caaaatcagg
     3361 aaggttttgt tcatatttgc cggctggata cggcgggcgc acgagtactg gaaaactaaa
     3421 tgaaactcta caatctgaaa gatcacaatg agcaggtcag ctttgcgcaa gccgtaaccc
     3481 aggggttggg caaaaatcag gggctgtttt tcccgcacga cctgccggaa ttcagcctga
     3541 ctgaaattga tgagatgctg aagctggatt ttgtcacccg cagtgcgaag atcctctcgg
     3601 cgtttattgg tgatgaaatc ccgcaggaaa tcctggaaga gcgcgtacgc gcggcgtttg
     3661 tcttcccggc tccggtcgcc aatgttgaaa gcgatgtcgg ttgtctggaa ttgttccacg
     3721 ggccaacgct ggcatttaaa gatttcggcg gtcgctttat ggcacaaatg ctgacccata
     3781 ttgcgggcga taagccagtg accattctga ccgcgacctc cggtgatacc ggagcggcag
     3841 tggctcatgc tttctacggt ttgccgaatg tgaaagtggt tatcctctat ccacgaggca
     3901 aaatcagtcc actgcaagaa aaactgttct gtacattggg cggcaatatt gaaactgttg
     3961 ccatcgacgg cgatttcgat gcctgtcagg cgctggtgaa gcaggcgttt gatgatgaag
     4021 agctgaaagt ggcgctgggg ttaaactcgg ctaactcgat taacatcagc cgtttgctgg
     4081 cgcagatttg ctactacttt gaagctattg cgcagctgcc gcaggaagcg cgcaaccagc
     4141 tggttgtctc ggtgccaagc ggaaacttcg gcgatttgac ggcgggtctg ctggcgaagt
     4201 cactcggtct gccggtgaaa cgttttattg ctgcgaccaa cgtgaacgat accgtgccac
     4261 gtttcctgca cgacggtcag tggtccccca aagcgactca ggcgacgtta tccaacgcga
     4321 tggacgtgag tcagccgaac aactggccgc gtgtggaaga gttgttccgc cgcaaaatct
     4381 ggcaactgaa agagctgggt tatgcagccg tggatgatga aaccacgcaa cagacaatgc
     4441 gtgagttaaa agaactgggc tacacctcgg agccgcacgc tgccgtagct tatcgtgcgc
     4501 tgcgtgacca gttgaatcca ggcgaatatg gcttgttcct cggcaccgcg catccggcga
     4561 aatttaaaga gagcgtggaa gcgattctcg gtgaaacgtt ggatctgcca aaagagctgg
     4621 cagaacgtgc tgatttaccc ttgctttcgc ataatctgcc cgccgatttt gctgcgttgc
     4681 gtaaattgat gatgaatcat cagtaatatc tattcattat ctcaatcagg ccgggtttgc
     4741 ttttatgcag cccggctttt ttgtgaagaa aatatggaga gaaacgacag ggaaaaagga
     4801 gaaattctca ataaatgcgg taacttagag attaggattg cggagaataa caaccgccgt
     4861 tctcatcgcg taatctccgg atatcgaccc ataacgggca atgataaaag gagtaacctg
     4921 tgaaaaagat gcaatctatc gtactcgcac tttccctggt tctggtcgct cccatggcag
     4981 cgcaggctgc ggaaattacg ttagtcccgt cagtaaaatt acagataggc gatcgtgata
     5041 atcgtggcta ttactgggat ggcggtcact ggcgcgacca cggctggtgg aaacaacatt
     5101 atgaatggcg aggcaatcgc tggcacccat atggaccgcc gccatcgccg cgccatgaca
     5161 agcacaatga tcatcgtggc gatcatcgtc cggggcctga caaacatcat cgctaaatat
     5221 caatgccgga ttgccatccg gcattggcta caaacttaac gctgctcgta gcgtttaaac
     5281 accagttcgc cattgctgga ggaagcttca tcaaagaagt aaccttcgct attaaaacca
     5341 gtcagttgct ctggtttggt cagccgattt tcaataataa aacgactcat cagaccgcgt
     5401 gctttcttag cgtagaagct gattatctta aatttgccgt tcttctcatc gaggaacacc
     5461 ggcttgataa tctcggcatt caatttcttc ggcttcaccg atttaaaata ctcatctgac
     5521 gccagattaa tcaccacatt atcgccttgt gctgcgagcg cctcgttcag cttgttggtg
     5581 atgatatctc cccagaattg atacagatct ttccctcggg cattctcaag acggatcccc
     5641 atttccagac gataaggctg cattaaatcg agcgggcgga gtacgccata caagccggaa
     5701 agcattcgca aatgctgttg ggcaaaatcg aaatcgtctt cgctgaaggt ttcggcctgc
     5761 aagccggtgt agacatcacc tttgaatgcc agaatcgcct ggcgggcatt cttcggcgtg
     5821 aaatctggct gccagtcatg aaagcgagcg gcgttgatac ccgacagttt gtcgctgatg
     5881 cgcatcagcg tgctaatctg cggaggcgtc agtttccgcg cttcatggat caactgctgg
     5941 gaattgtcta acagctccgg cagcgtatag cgcgtggtgg gcaacgggct ttggtaatcg
     6001 agcgttttcg caggtgaaat aagaatcagc atatccagtc cttgcaggaa atttatgccg
     6061 actttagcaa aaaaagagaa tgagttgatc gatagttgtg attactcctg cgaaacatca
     6121 tcccacgcgt ccggagaaag ctggcgaccg atatccggat aacgcaatgg atcaaacacc
     6181 gggcgcacgc cgagtttacg ctggcgtaga taatcactgg caatggtatg aaccacaggc
     6241 gagagcagta aaatggcggt caaattggta atagccatgc aggccattat gatatctgcc
     6301 agttgccaca tcagcggaag acttagcaag gtgccgccga tgaccgttgc gaaggtgcag
     6361 atccgcagac accagatcgc tttagggttg ttcaggcgta agaagaagag attgttttcg
     6421 gcataaatgt agttggcaac gatggagctg aaggcaaaca gaataaccac gagggtaaca
     6481 aactccgcac cccaggaacc cattaacacc cgcatcgcct tctggataag ctgaatacct
     6541 tccagtggca tgtaggttgt gccgttaccc gccagtaata tcagcatggc gcttgccgta
     6601 cagatgacca gggtgtcgat aaaaatgcca atcatctgga caattccttg cgctgcggga
     6661 tgcggaggcc aggacgcagc cgccgctgcc gcgtttggcg tcgaccccat tcccgcctca
     6721 ttggaaaaca tactgcgctg aaaaccgtta gtaatcgcct ggcttaaggt atatcccgcc
     6781 gcgccgcctg ccgcttcctg ccagccaaaa gcactctcaa aaatagacca aatgacgtgg
     6841 ggaagttgcc cgatattcat tacgcaaatc accaggctgg tcagtaccca gattatcgcc
     6901 atcaacggga caaacccctg catgagctgg gcgacgccat gaagaccgcg agtgattgcc
     6961 agcagagcaa agacagcgag aataatgcct gtcaccagcg gaggaaaatc aaaagaaaaa
     7021 ctcagggctc gggcaacggc gttcgcctga actccgctga aaattatgcc gtaggcgatg
     7081 agcaaaaaga gggcgaacag aacgcccatc caacgcatcc ccagcccgcg cgccatatac
     7141 catgccggtc cgccacgaaa ctgcccattg acgtcacgtt ctttataaag ttgtgcaagg
     7201 gaacattcgg caaacgaggt cgccatgccg ataaacgcgg caacccacat ccagaagacg
     7261 gctccaggtc caccggcggt aatagccagc gcaacgccag ccaggttgcc gctacccacg
     7321 cgcgccgcaa gactggtaca caatgactga aatgaggtta aaccgcctgg ctgtggatga
     7381 atgctatttt taagactttt gccaaactgg cggatgtagc gaaactgcac aaatccggta
     7441 cgaaaagtga accaacaacc tgcgccgaag agcaggtaaa tcattaccga tccccaaagg
     7501 acgctgttaa taaaggagaa aaaatctggc atgcatatcc ctcttattgc cggtcgcgat
     7561 gactttcctg tgtaaacgtt accaattgtt taagaagtat atacgctacg aggtacttga
     7621 taacttctgc gtagcataca tgaggttttg tataaaaatg gcgggcgata tcaacgcagt
     7681 gtcagaaatc cgaaacagtc tcgcctggcg ataaccgtct tgtcggcggt tgcgctgacg
     7741 ttgcgtcgtg atatcatcag ggcagaccgg ttacatcccc ctaacaagct gtttaaagag
     7801 aaatactatc atgacggaca aattgacctc ccttcgtcag tacaccaccg tagtggccga
     7861 cactggggac atcgcggcaa tgaagctgta tcaaccgcag gatgccacaa ccaacccttc
     7921 tctcattctt aacgcagcgc agattccgga ataccgtaag ttgattgatg atgctgtcgc
     7981 ctgggcgaaa cagcagagca acgatcgcgc gcagcagatc gtggacgcga ccgacaaact
     8041 ggcagtaaat attggtctgg aaatcctgaa actggttccg ggccgtatct caactgaagt
     8101 tgatgcgcgt ctttcctatg acaccgaagc gtcaattgcg aaagcaaaac gcctgatcaa
     8161 actctacaac gatgcaggta ttagcaacga tcgtattctg atcaaactgg cttctacctg
     8221 gcagggtatc cgtgctgcag aacagctgga aaaagaaggc atcaactgta acctgaccct
     8281 gctgttctcc ttcgctcagg ctcgtgcttg tgcggaagca ggcgtgttcc tgatctcgcc
     8341 atttgttggc cgtattcttg actggtacaa agcgaatact gataagaaag agtacgctcc
     8401 ggcagaagat ccgggcgtgg tttctgtatc tgaaatctac cagtactaca aagagcacgg
     8461 ttatgaaacc gtggtgatgg gcgcaagctt ccgtaacatc ggcgaaattc tggaactggc
     8521 aggctgcgac cgtctgacca tcgcaccggc gctgctgaaa gagctggctg agagcgaagg
     8581 ggctatcgaa cgtaaactgt cttacaccgg cgaagtgaaa gcgcgtccgg cgcgtatcac
     8641 tgagtccgag ttcctgtggc agcataacca ggatccaatg gcagtagata aactggcgga
     8701 aggtatccgt aagtttgctg ttgaccagga aaaactggaa aaaatgatcg gcgatctgct
     8761 gtaatcattc ttagcgtgac cgggaagtcg gtcacgctac ctcttctgaa gcctgtctgt
     8821 cactcccttc gcagtgtatc attctgttta acgagactgt ttaaacggaa aaatcttgat
     8881 gaatacttta cgtattggct tagtttccat ctctgatcgc gcatccagcg gcgtttatca
     8941 ggataaaggc atccctgcgc tggaagaatg gctgacatcg gcgctaacca cgccgtttga
     9001 actggaaact cgcttaatcc ccgacgagca ggcgatcatc gagcaaacgt tgtgtgagct
     9061 ggtggatgaa atgagttgcc atctggtgct caccacgggc ggaactggcc cggcgcgtcg
     9121 tgacgtaacg cccgatgcga cgctggcagt agcggaccgc gagatgccag gctttggtga
     9181 acagatgcgc cagatcagcc tgcattttgt accaactgcg atcctttcgc gtcaggtggg
     9241 tgtgattcgc aaacaggcgc tgatccttaa cttacccggt cagccgaagt ctattaaaga
     9301 gacgctggaa ggtgtaaagg acgctgaggg taatgttgtg gtgcacggta tttttgccag
     9361 cgtaccgtac tgcattcagt tgctggaagg gccatacgtt gaaacggcac cggaagtggt
     9421 tgctgcattc agaccgaaga gtgcaagacg cgaagttagc gaataaaaaa atccccccga
     9481 gcggggggat ctcaaaacaa ttagtgcgat tcaccaatcg gcagaacggt gcgaccaaac
     9541 tgctcgttca gcacttcacc catcgccaga tagattgcgc tggcaccgca gatcagacca
     9601 atccagccgg caaagtggat gatcgcggcg ttaccggcaa tgttaccgat cgccagcagg
     9661 gcaaacagca cggtcaggct aaagaaaacg aattgcagaa cgcgtgcgcc tttcagcgtg
     9721 ccgaagaaca taaacagcgt aaatacgccc cacagaccca ggtagacacc aaggaactgt
     9781 gcatttggcg catcggtcag acccagtttc ggcatcagca gaatcgcaac cagcgtcagc
     9841 cagaaagaac cgtaagaggt gaatgcggtt aaaccgaaag tgttgccttt tttgtactcc
     9901 agcagaccag caaaaatttg cgcgatgccg ccgtagaaaa tgcccatggc aagaataata
     9961 ccgtccagag cgaaataacc cacgttgtgc aggttaagca gaatggtggt catgccgaag
    10021 cccatcaggc ccagcggtgc cggattagcc aacttagtgt tgcccataat tcctcaaaaa
    10081 tcatcatcga atgaatggtg aaataatttc cctgaataac tgtagtgttt tcagggcgcg
    10141 gcataataat cagccagtgg ggcagtgtct acgatctttt gaggggaaaa tgaaaatttt
    10201 ccccggtttc cggtatcaga cctgagtggc actaaccatc cggcgcaggc aggcgatttg
    10261 cagtacggct ggaatcgtca cgcgataggc gctgccgctg accgctttaa ccccatttag
    10321 tgccgcgcct acagggcctc ccagccccgc gccgcgcagc aaaccatgcc caagtacgct
    10381 cattgctgcg tgggtgcgta aaatgcgggt caattggctg gaaagcaaat gcgacacgcc
    10441 ttttgccaat aatttgtctt tcatcagcag cggcagcagc tcttccagct cattcaccct
    10501 ggcatcgacc gcgtgcagaa actcctgctt atgttcctcg tccattttct tccaggtatt
    10561 acgcagaaat tgctccagta actgttgctc aatttcaaac gtagacatct ctttgtcggc
    10621 tttcagcttc aatctttttg aaacatcgag caaaatggcc cgatacaatt taccgtgtcc
    10681 gcgcaatttg ttggcgatac tatcgccacc aaaatgttgt aattctccgg caatcagctg
    10741 ccagttgcgg cggtgttgct cgggatgtcc ttccatcgat ttaaacagtt cgttgcgcat
    10801 cagtacgctg gagaggcgag ttttgccttt ttcattatga gtgagcagcc gggcgaaatt
    10861 tgccaattgt tcctcactac aatgctggag aaaatccaga tctgaatcat tcaggtaatt
    10921 aacattcatt ttttgtggct tctatattct ggtgttagtc gtcgccgata attttcagcg
    10981 tggccatatc cgatgagttc accgtatggc cggaaaaggt gatttttgag acgcagcgtt
    11041 tattgtcgtt atcgctgtta atgttgatcc agtcagtggt ttgcccttct tttatttctg
    11101 aaggaatatt caggctctga ctggcgctac gggcggcttt gaaataaacc gatgcaccac
    11161 ttaactgtaa atcgccatgg tcggcagtga gttgaatgcg tttaacaatg cgacaaacag
    11221 gaagtttcag cgccagatcg ttggtttcgt tacgcggcat cgcaatgacg ccgaggattt
    11281 tatggttgtt tgcctgcgct gtgcagcaca gcagcaggct aatcgccagg ctggcggaaa
    11341 gcgtaaaaac ggatttcata aggattctct tagtggggag aggtagggga tgaataccca
    11401 ctagtttact gctgataaag agaagattca ggtacgtaat attttctttt tattacaatt
    11461 tttggtgaat accttggctg agattcattc tttatatgaa taaaattgct gtcagtttca
    11521 cgtcttgttc tgccatatcg cgaaatttct gcgcaaaagc acaaaaaatt tttgcatctc
    11581 ccccttgatg acgtgggtta cgaccccatt tagtagtcaa ccgcagtgag tgagtctgca
    11641 aaaaaatgaa attgggcagt tgaaaccaga cgtttcgccc ctattacaga ctcacaacca
    11701 catgatgacc gaatatatag tggagacgtt tagatgggta aaataattgg tatcgacctg
    11761 ggtactacca actcttgtgt agcgattatg gatggcacca ctcctcgtgt actggagaac
    11821 gccgaaggcg atcgcaccac gccttctatc attgcctata cccaggatgg tgaaactctg
    11881 gttggtcagc cggctaaacg tcaggcagtg acgaacccgc aaaacaccct gtttgcgatt
    11941 aaacgcctga ttggccgccg cttccaggac gaagaagtac agcgtgatgt ttccatcatg
    12001 ccgttcaaaa ttattgctgc tgataacggc gacgcatggg tcgaagttaa aggccagaaa
    12061 atggcaccgc cgcagatttc tgctgaagtg ctgaaaaaaa tgaagaaaac cgctgaagat
    12121 tacctgggtg aaccggtaac tgaagctgtt atcaccgtac cggcatactt taacgatgct
    12181 cagcgtcagg caaccaaaga cgcaggccgt atcgctggtc tggaagtaaa acgtatcatc
    12241 aacgaaccga ccgcagctgc gctggcttac ggtctggaca aaggtactgg caaccgtact
    12301 atcgcggttt atgacctggg tggtggtact ttcgatattt ctattatcga aatcgacgaa
    12361 gttgacggcg aaaaaacctt cgaagttctg gcaaccaacg gtgatactca cctgggtggt
    12421 gaagacttcg acagccgtct gatcaactac ctggttgaag aattcaagaa agatcagggc
    12481 attgacctgc gcaacgatcc gctggcaatg caacgcctga aagaagcggc agaaaaagcg
    12541 aaaatcgaac tgtcttccgc tcagcagacc gacgttaacc tgccgtacat cactgcagac
    12601 gcgaccggtc cgaaacacat gaacatcaaa gtgactcgtg cgaaactgga aagcctggtt
    12661 gaagatctgg taaaccgttc cattgagccg ctgaaagttg cactgcagga cgctggcctg
    12721 tccgtatctg atatcgacga cgttatcctc gttggtggtc agactcgtat gccaatggtt
    12781 cagaagaaag ttgctgagtt ctttggtaaa gagccgcgta aagacgttaa cccggacgaa
    12841 gctgtagcaa tcggtgctgc tgttcagggt ggtgttctga ctggtgacgt aaaagacgta
    12901 ctgctgctgg acgttacccc gctgtctctg ggtatcgaaa ccatgggcgg tgtgatgacg
    12961 acgctgatcg cgaaaaacac cactatcccg accaagcaca gccaggtgtt ctctaccgct
    13021 gaagacaacc agtctgcggt aaccatccat gtgctgcagg gtgaacgtaa acgtgcagct
    13081 gataacaaat ctctgggtca gttcaacctg gatggtatca acccggcacc gcgcggcatg
    13141 ccgcagatcg aagttacctt cgatatcgat gctgacggta tcctgcacgt ttccgcgaaa
    13201 gataaaaaca gcggtaaaga gcagaagatc accatcaagg cttcttctgg tctgaacgaa
    13261 gatgaaatcc agaaaatggt acgcgacgca gaagctaacg ccgaagctga ccgtaagttt
    13321 gaagagctgg tacagactcg caaccagggc gaccatctgc tgcacagcac ccgtaagcag
    13381 gttgaagaag caggcgacaa actgccggct gacgacaaaa ctgctatcga gtctgcactg
    13441 actgcactgg aaactgctct gaaaggtgaa gacaaagccg ctatcgaagc gaaaatgcag
    13501 gaactggcac aggtttccca gaaactgatg gaaatcgccc agcagcaaca tgcccagcag
    13561 cagactgccg gtgctgatgc ttctgcaaac aacgcgaaag atgacgatgt tgtcgacgct
    13621 gaatttgaag aagtcaaaga caaaaaataa tcgccctata aacgggtaat tatactgaca
    13681 cgggcgaagg ggaatttcct ctccgcccgt gcattcatct aggggcaatt taaaaaagat
    13741 ggctaagcaa gattattacg agattttagg cgtttccaaa acagcggaag agcgtgaaat
    13801 caaaaaggcc tacaaacgcc tggccatgaa ataccacccg gaccgtaacc agggtgacaa
    13861 agaggccgag gcgaaattta aagagatcaa ggaagcttat gaagttctga ccgactcgca
    13921 aaaacgtgcg gcatacgatc agtatggtca tgctgcgttt gagcaaggtg gcatgggcgg
    13981 cggcggtttt ggcggcggcg cagacttcag cgatattttt ggtgacgttt tcggcgatat
    14041 ttttggcggc gggcgtggtc gtcaacgtgc ggcgcgcggt gctgatttac gctataacat
    14101 ggagctcacc ctcgaagaag ctgtacgtgg cgtgaccaaa gagatccgca ttccgactct
    14161 ggaagagtgt gacgtttgcc acggtagcgg tgcaaaacca ggtacacagc cgcagacctg
    14221 tccgacctgt catggttctg gtcaggtgca gatgcgccag ggtttctttg ccgtgcagca
    14281 gacctgtcca cactgtcagg gccgcggtac gctgatcaaa gatccgtgca acaaatgtca
    14341 tggtcatggt cgtgttgagc gcagcaaaac gctgtccgtt aaaatcccgg caggagtgga
    14401 cactggagac cgcatccgtc ttgcgggtga aggtgaagcg ggcgagcatg gcgcaccggc
    14461 aggcgatctg tacgttcagg ttcaggttaa acagcacccg attttcgagc gtgaaggtaa
    14521 caacctgtat tgcgaagtcc cgatcaactt cgctatggca gcgctgggtg gcgaaatcga
    14581 agtaccgacc cttgatggtc gcgtcaaact gaaagtgcct ggcgaaaccc agaccggtaa
    14641 gctgttccgt atgcgcggta aaggcgtcaa gtctgttcgc ggcggtgcgc aaggtgactt
    14701 gctgtgccgc gttgtcgtcg aaacaccggt aggtctgaac gagaagcaga aacagctgct
    14761 gcaagagctg caggaaagct tcggtggccc aaccggtgag cacaacagcc cgcgttcaaa
    14821 gagcttcttt gatggtgtga agaagttttt tgacgacctg actcgctaac ctccccaaaa
    14881 gcctgcctgt gggcaggcca gggtaaaaat agggtgcgtt gaagatatgc gagcacctgt
    14941 aaagtggcgg ggatcactcc ccgccgttgc tcttactcgg attcgtaagc cgtgaaaaca
    15001 gcaacctccg tctggccagt tcggatgtga acctcacaga ggtcttttct cgttaccagc
    15061 gccgccacta cggcggtgat acagatgacg atcagggcga caatcatcgc cttatgctgc
    15121 ttcattgctc tcttctcctt gaccttacgg tcagtaagag gcactctaca tgtgttcagc
    15181 atatagggag cctcgggttg atggtaaaat atcactcggg gcttttctct atctgccgtt
    15241 cagctaatgc ctgagacaga tagcctcaag cacccgccgc tattatatcg ctctctttaa
    15301 cccattctgt tttatcgatt ctaatcctga agacgcctcg tatttttatg gcgtaatttt
    15361 ttaatgattt aattatttaa ctttaattta tctcttcatc gcaattattg acgacaagct
    15421 ggattatttt tgaaatattg gcctaacaaa catcgccgac tgacaacaaa ttaattatta
    15481 cttttcctaa ttaatccctc agaaatcctg accttaagct atgattatca gggcttaggg
    15541 tcactcgtga gcgcttacag ccgtcaaaaa ctcatctcat cgctaatggc acaaatcctt
    15601 caatggttcg taaaaaacga attattccta cactataatc tgattttaac gatgattcgt
    15661 gcggggtaag atagtaaaaa cgatctattc acctgaaaga gaaataaaaa gtgaaacatc
    15721 tgcatcgatt ctttagcagt gatgcctcgg gaggcattat tcttatcatt gccgctatcc
    15781 tggcgatgat tatggccaac agcggcgcaa ccagtggatg gtatcacgac tttctggaga
    15841 cgccggttca gctccgggtt ggttcactcg aaatcaacaa aaacatgctg ttatggataa
    15901 atgacgcgct gatggcggta tttttcctgt tagtcggtct ggaagttaaa cgtgaactga
    15961 tgcaaggatc gctagccagc ttacgccagg ctgcatttcc agttatcgcc gctattggtg
    16021 ggatgattgt gccggcatta ctctatctgg cctttaacca tgccgatccg attacccgcg
    16081 aagggtgggc gatcccggcg gctactgaca ttgcttttgc acttggtgtg ctggcgctgt
    16141 tgggaagtcg tgttccgtta gcgctgaaga tctttttgat ggctctggct attatcgacg
    16201 atcttggggc catcattatc atcgcattgt tctacactaa tgacttatcg atggcctctc
    16261 ttggtgtcgc ggctgtagca attgcggtac tcgcggtatt gaatctgtgt ggtgtacgcc
    16321 gcacgggcgt ctatattctg gttggcgtgg tgttgtggac tgcggtgttg aaatcggggg
    16381 ttcacgcaac cctggcgggg gtaattgtcg gcttctttat tcctttgaaa gagaagcatg
    16441 ggcgttctcc agcgaagcga ctggagcatg tgttgcaccc gtgggtggcg tatctgattt
    16501 taccgctgtt tgcatttgct aatgctggcg tttcactgca aggcgtcacg ctggatggct
    16561 tgacctccat tctaccattg gggatcatcg ctggcttgct gattggcaaa ccgctgggga
    16621 ttagtctgtt ctgctggttg gcgctgcgtt tgaaactggc gcatctgcct gagggaacga
    16681 cttatcagca aattatggcg gtggggatcc tgtgcggtat cggttttact atgtctatct
    16741 ttattgccag cctggccttt ggtagcgtag atccagaact gattaactgg gcgaaactcg
    16801 gtatcctggt cggttctatc tcttcggcgg taattggata cagctggtta cgcgttcgtt
    16861 tgcgtccatc agtttgacag gacggtttac cggggagcca taaacggctc ccttttcatt
    16921 gttatcaggg agagaaatga gcatgtctca tatcaattac aaccacttgt attacttctg
    16981 gcatgtctat aaagaaggtt ccgtggttgg cgcagcggag gcgctttatt taacaccaca
    17041 aaccattacc gggcagattc gggcgctgga agagcgcctg caaggcaaat tatttaaacg
    17101 caagggacgt ggtctcgaac ccagcgagct gggagagctg gtctatcgct atgccgataa
    17161 aatgttcacc ttaagccagg aaatgctgga tattgtgaac tatcgcaaag aatccaattt
    17221 attgtttgac gttggcgtgg ctgatgcact ttctaaacgc ctggtcagta gcgtacttaa
    17281 cgccgcagtg gtagaaggcg agccgattca tcttcgctgc ttcgaatcca cccacgaaat
    17341 gctgctggag caattaagtc agcataaact ggatatgatc atttccgact gtccgataga
    17401 ttctacgcag caggaaggtc tgttctccgt gagaattggc gaatgtggcg tgagtttctg
    17461 gtgtacaaat ccaccaccgg aaaaaccgtt cccggcttgt ctggaagaac ggcggctttt
    17521 gattcctggg cgacgttcaa tgttagggcg caaattgctt aactggttta actcccaggg
    17581 attaaacgta gaaatcctcg gcgagtttga tgatgccgcg ttgatgaaag cttttggtgc
    17641 gatgcacaat gcaatcttcg ttgccccaac gctttatgca tatgactttt atgccgataa
    17701 aactgtcgta gaaattggtc gcgtcgaaaa tgtgatggaa gagtaccatg ctatttttgc
    17761 tgagcggatg attcagcacc cggcggtaca gcgaatctgc aatacggatt attctgcgct
    17821 ttttagccca gcggcacgtt aatcggcagc tcccccaaag ttaaggtggg ggagatagat
    17881 tagttgtaca ttaccacgat tttgactcgg ctcattattt gcccgcttga gacattattt
    17941 ccatatgtac gcgggcgaat aaatagagga atctgattac ttccttcatg gggatgctga
    18001 aaagagtagt aattgctttt accatactgc atttcattcg taccatcatt ggagagttga
    18061 aagcctattt gacccgcacc gtcctcactg ttttctaaat cattttttaa tatgctggtt
    18121 cctgaatcct gtatcgtcac tggctcaaag taatattcaa catcactagg ttcaaaaact
    18181 aattcacctt gctcaccctg gatacagtca aatcttatat gatgaaactg ataaatttca
    18241 ttattgatac gagacagtac aaatcgtcca gtgtcgatgg attgatcttc aaaaccacag
    18301 gtcattaaat ttacctgtaa aatagtattt aaattaaaat taaagcggac gctattacaa
    18361 ttagtattat tccctgtaca tgaactcact tcaatttcga acagcaaagg aaaaggagtt
    18421 cctggaaaaa tatctgcggt gtcagaacta tctgatacag atgcgataga atatggaatg
    18481 gtgaaattta tcgcagatag catttcttct aaattataag ataacttccc tggaagctct
    18541 atttttttac cgtcagattc aaaggggaaa ttaataatgt aatgacgact tccgacttta
    18601 aaccttaaag cgttctgtgt taaaaaatga actttaatgg cgttggtcgt ctcttgcaag
    18661 tctgcacact caatgatatt cccggtgttt tcaattttct cggcagttag attacctttc
    18721 atactcagtg gcttgagtat atatgttcca cgttgttcac cgacattggt cactgcacag
    18781 ggagcacttt gcacaataaa agaatataga cttaaggtga ttaataaaag ccacttcata
    18841 gtaaactcct tttactggca tatatatgtt ttgttttcat cgataacgtg ttgaaaagta
    18901 atagtacaga ataagttctg ttcatcattg agagcaactt ttatgccagt gggaatttca
    18961 tctacgcgaa taaaaaggcg actaccctgg ccaacgactc caatattgtt ttcctggaga
    19021 tccagtacgt catagccaaa ggttaatggc gaaccatcgg tacgcagtgc ctgtatatac
    19081 cagggcttac gttggtcagt tgtaaactgc acataagaaa ctgctccacg gtaaggcgca
    19141 ataatttgac gattgccctg caattctgtt tcactttcac tgtttgctat atcaagtacc
    19201 agatggtttt cacgatgagg tatcatcagg tcgtaaacaa cctggccttt gctgtttgtt
    19261 cggttgtatt tttgtccatt aatatgcgcg ccttctaacc caggcgcatc cagaattgca
    19321 aatgtatcgg ataactggtt agttatatta ataccaccgg gccagacaac taatccacta
    19381 ctaatacttc caccactttg ccaggcgttt ttagaatggc tatagctgcc atctattgtg
    19441 gcgatgggag ttctccagct taaattcgta ccaagcgagg tatcattatt ttgttgttgc
    19501 tgattaacat atataccata atttaactga tcatgttcac cggctatgcc agtaattcca
    19561 gtattgttgg aggaatagcc atctttggaa aatgaggtcg aattcgataa gttaatttgg
    19621 tgacgtgttt tggcaatatc atccccccag tagaaaggaa tggagataaa cagattaaaa
    19681 cgctcctctt ctttatcgtt ttcatcatag gattggctgg cagagaaagt ataactaatg
    19741 cgttgccagc tatttgaata actgaattgg taatctttag catttccact tcgcccccag
    19801 taattccgcc aaagcgcact taatgataca tttcccagat tattggataa aggttgcata
    19861 atattggcag aaaggctatt ttttctgcca atatcataaa aatcatcata ttcactctga
    19921 tgattgtgtt tatcattttc gtaaagatgg tcgctgaacg tcctgtaatc ctgcgaagca
    19981 taacgccagg cggccacgct aaagcgagta gccgtttgca ctagatattt attataggcg
    20041 acctgatagc tcgtaccttc gtgtgttata tcattattta acttactact cgatcgggta
    20101 gcgtcaaaag agatggcacc cagcggtgta ttccaaccat ttcccagggt tatagcatta
    20161 tagttatcgg ataaaatcgt accgccataa agtgtcaaaa gattgttgag gccatatata
    20221 taatttgcct cgagaaagtc ctcctgattc tttacaccat atatcttact gcgtccggcg
    20281 ataaaatcga aatttgaaac accaggttgc agcatgttgg gtacggaaga gtaggggacc
    20341 aaaaaggaac gaacactacc atctgcttcc tttatgctga catcgagatc tgagccgctg
    20401 cctgaaagtt gcaaatctgc gatagtaaat ggcccggggg gaacctcttt ttggtaaatg
    20461 atgtagccat tttgcgaaac cgttatcaag gcaatacttt gcgcaacacc ttgtactaaa
    20521 ggtgtaaagc tctgcataga atcaggtagc atttgcatat cgcgaaataa gcgtattccg
    20581 ctaaatcgca gagagtcgaa tatgagtgag gatgtgtaat tttctcctat ctgaactgtg
    20641 cttaatattt gcgaccagcc acgttccagg taaagagtat tactttgcca tgtgcctttc
    20701 atatcatcag gcttgctata gctggcatca gagtgtaatt gccaactaaa taaatttaat
    20761 ccgctgttaa aacgaccata actggctgta ttactattat tattgctgtc ataagagcga
    20821 tactgactta aattatagga ggtataaaaa gcatcaatgc cgcgatccca actttccggg
    20881 ggaacgtaac ctctttcaag ttcattgata tacgcctgtg ggatcgttaa atttagtcgg
    20941 tgttcactga tatcccactg atattgtcca ccatgaaccg cttcatccag taaaatacaa
    21001 ttatcttctg tattgagatt atcggttttt acccccagtg tggttagtag caccttaggc
    21061 agacatggtt tggctggtga ctctgggatc gtgatatctt gtttcccacg ccactggtgg
    21121 tttacataaa catcaagttc gtagtttccc ggaagaggtt gtttattatc gaagcgatat
    21181 tcggaaactt tttttcctct catgccgcca atcatgaagt gcgtatcaaa agtttcttca
    21241 gcataacccg gaaaagaata caaaaaaata atagcagcga cccatcgtgt cgtccttaac
    21301 atgacttttt cctgttatcg tgcggcgatt ttactatgga tgttattccc gtaatcatca
    21361 actaccgtta gttcatattc tgaggcgttg ttatttttta atgtaatgtc gttattcgaa
    21421 aatggaggca gtaggatact ttcattatta actttcacgt tttgtgcttt gatagttgta
    21481 actgttatcc agttggctgt ttcattgttt attgagatgg cgttgttttt ctttttgata
    21541 tttaattgag aaaggctttt tttatctacc ggagctattc cgcttggacg ccaaagtaac
    21601 tttatacggt tttgcaatgc aagtttaatt ttgttctttc ctgcattttg tgggttgttt
    21661 ggagggatat ccagaacatt tagataaaat aaactttctt tattgtgagg caaattgttt
    21721 ggcatttttc ttattttaag ttgttggccg gaaccacctg ctacacgact tactggcggt
    21781 gtgagaataa atgggacctg aattttttct ggcgcgatag tggtgttccc atcatcgatc
    21841 catgcctgaa tcaaagaggc atttttactt tgattcatta actgtacgag aacttctctg
    21901 cttttttccg gataaataat gcgcgtacca tagatgacca caccggcatg aacaggtaaa
    21961 ataaaaaatg acatcagtaa tgctgttatt attctcacgt aatcactccg taaataatac
    22021 cggtagcaga gctaccggca aaaaacgtta atctgcactg attttaatcg ttacggttga
    22081 ctctacggag ccttttgtaa caggattgcc acttgtgcgg gcatattgtg cacttaaatt
    22141 aagaaaatat tgaccagagc cattatcttt cagcggtgtt tttaaccccg gtctcattct
    22201 ggtcaaagtt ccatcatcga ggaaaatggc aaagccaatg ttctcagcag ttcctttatt
    22261 cagcgtaaag ttattagcat caaactctga tgctagcact tcgatacttg gggctgtatt
    22321 gccgtcacac tgaatttcta agtcatgaga ggttgaaccg gtttttaaag tatagatttc
    22381 attattgata agttgattca ttgcactggt tacaacatct tcgacaacga tatctgttgt
    22441 cgaatcacca ttaacaaagt aacacgttgt atcaacaact tctcctctaa tggttagcgt
    22501 tccttgatcg acatccttgg cgaaggttgt tgcgctaaac atcaacgttg tcgcaacgaa
    22561 gggaataata tactttttca taaatcatcc ttaattaact ggtatggaag gtcactcgtt
    22621 gtcaaacccg attatgtgat caggttgtta ttatttagat taatgtttaa attttttaat
    22681 ttcttgatgt gaagttgatg aaactttgaa aatttaatat tcttaattat tcccttcttt
    22741 ataagaagag caaggtgcca tcattagagt gacagagtgg gatgtttaag atcatgcttc
    22801 agattatttt ttgtttctct gcagtgaatt aataatcatc acagataatt tcattctgaa
    22861 attacttctt ttgggagtgt tttaatcttg atgaactatg tttttcataa ggaacataca
    22921 ctcgttgatg cttttaaggt attttctcat ttctgaggat gttttgtcgt aatcgagcat
    22981 aaaattatta agttctttat acacatcatt atttagcttt acattgtcgc ggactaaagt
    23041 ttgtatcaat ggtatcaaaa tttcgaaacg ggacggtttg tcatcaatac ttgccagggt
    23101 tttgaatact cctgtgtatc tgtaacctaa gtgcttattg acaagttgag tgttccatcg
    23161 accaggcata atatcttcac agcacaaatt ttttatcctg gtattagtga acgatgttcc
    23221 aaatatgttg ctttttttaa tgaagaggtt atttatttct ccattcgaaa agatggtgtt
    23281 tgataagtca ctttcaaaac atgatatatt ggataataca caaccaaaat aatacccata
    23341 tgaaagattg ctcttttcta ttgttatatt tgatagcaat agtttattta tattaatatt
    23401 atgggtaatc aaatactgaa ttttttgctc gttaagttca tacatctcca gtgatttatt
    23461 atagggtaat aaaagtataa attcctttat attatctccg cagtaagaaa agacaacgcc
    23521 aaatttattg gtgaccatgt tttcagttga gcagtaagct gatttttcta ttaaccgact
    23581 gggcacgctt ttaaataaag atgatttttc acatagttgt gaatatgccg cattgctagt
    23641 tttaaaattc cgccatgtta tttcctggtt atcatctgtc gatgaggcgg ggattttcaa
    23701 gcattgttca ttgtatag
//
LOCUS       DAPOZO010000076        23415 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPOZO010000076 DAPOZO010000000
VERSION     DAPOZO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.076"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            1..299
                     /locus_tag="ROI21_003275"
                     /pseudo
     CDS             1..299
                     /locus_tag="ROI21_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(404..1453)
                     /locus_tag="ROI21_003276"
     CDS             complement(404..1453)
                     /locus_tag="ROI21_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated sensor domain-containing
                     protein"
                     /protein_id="HDX5112163.1"
                     /translation="MVIRGMTISLPWFAFINVSFALIILLRRVLFNDLTPPWLNEKSL
                     IHSIDISATGILLICSGLLLIPRQKTLPIQVLLVALSLLWSWCSYHFIAYWTLQFAYP
                     LCVLLMLSGVVALYFHTPSLLAFVIPLWFTTPIASLMLNQQINIHFAVVWCIFSLALY
                     GGRLILLRWFEEAWIQNSYNNQLINRLDALAHRDPLTGIANRRAMNSILHDAMDNGGS
                     FALIMLDVDFFKRYNDTYGHPAGDRCLIQVADALQRSTRQSEDVVVRYGGEEFAIILF
                     NATLPEAETVAARVKQELKLAAIPHQASAVNAFVTVSQGITCSAPAKTAEQIISDADA
                     ALYRAKESGRNRWAL"
     gene            complement(1548..3524)
                     /locus_tag="ROI21_003277"
     CDS             complement(1548..3524)
                     /locus_tag="ROI21_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDX5112164.1"
                     /translation="MQLNHIVKSLLITGLFTTSSLPLLAAEAPKDATAATQQANNLLY
                     NQLPFADNTDFTDAHKGFIAPIPQDVIKGEQGNVIWDPQHYAFIKEGDKAPDTVNPSL
                     WRQAQLINISGLFEVTEGVYQIRNLDLSNMTIIEGKEGITVVDPLVSAETAKVGMDLY
                     YKNRGKKPVVAVIYTHSHVDHYGGVRGVIDEADVKSGKVKIYAPAGFLEEAVSENIMA
                     GNVMSRRASYMYGNLLKPDVKGQVGAGLGTTTSAGTVTLIAPTNYITKTGQIETIDGL
                     TYDFLMAPGSEAPSEMLWFIEEKKLIETAEDVTHTLHNTYSLRGAKIRQPLPWSKYIN
                     EALNLWGDKAEIILAQHHWPTWGNDNVVKLLKSQRDLYRYINDQTLRMANQGMTRDEI
                     AANFKLPSSLANTWANRGYYGSVSHDVKATYVLYLGWFDGNPATLDELPPEEGAKKFV
                     EYMGGADAILQKATQDYDQGNFRWVAQVVSKVVFADPNNQAARDLEADALEQLGYQAE
                     SGPWRNFYLTGAQELRNGVQKLPTPNTASPDTVRAMTPEMFFDYLAVHINGEKAADAK
                     TVLNFDFGEDGGTYKVELENGVLNHTAGVEASDADATITLSRDVLNKIVLKEETLKEA
                     TAKGDVKITGNEEKLNELLGYMDNFEFWFNIVTP"
     gene            3830..4057
                     /gene="pptA"
                     /locus_tag="ROI21_003278"
     CDS             3830..4057
                     /gene="pptA"
                     /locus_tag="ROI21_003278"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDX5112165.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(4061..4630)
                     /locus_tag="ROI21_003279"
     CDS             complement(4061..4630)
                     /locus_tag="ROI21_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDX5112166.1"
                     /translation="MSRFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGRDEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            4805..5650
                     /gene="nhoA"
                     /locus_tag="ROI21_003280"
     CDS             4805..5650
                     /gene="nhoA"
                     /locus_tag="ROI21_003280"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDX5112167.1"
                     /translation="MTPILNHYFARINWLGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVTARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVEVEGEKWIADVGFGGQTLTAPIRLVPELVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQLNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDDLALVMAAFDTH
                     PEAGK"
     gene            complement(5769..6662)
                     /locus_tag="ROI21_003281"
     CDS             complement(5769..6662)
                     /locus_tag="ROI21_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDX5112168.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRSKVLGLGNCTVWQTSLAGR
                     HRVTVEKQNDDYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(6741..7421)
                     /gene="narI"
                     /locus_tag="ROI21_003282"
     CDS             complement(6741..7421)
                     /gene="narI"
                     /locus_tag="ROI21_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDX5112169.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMIILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            complement(7418..8113)
                     /gene="narW"
                     /locus_tag="ROI21_003283"
     CDS             complement(7418..8113)
                     /gene="narW"
                     /locus_tag="ROI21_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDX5112170.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEGFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDTLLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            complement(8113..9657)
                     /gene="narH"
                     /locus_tag="ROI21_003284"
     CDS             complement(8113..9657)
                     /gene="narH"
                     /locus_tag="ROI21_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDX5112171.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGANWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            complement(9654..13394)
                     /gene="narZ"
                     /locus_tag="ROI21_003285"
     CDS             complement(9654..13394)
                     /gene="narZ"
                     /locus_tag="ROI21_003285"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDX5112172.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVNGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKNNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     QELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKREGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            complement(13489..14877)
                     /gene="narU"
                     /locus_tag="ROI21_003286"
     CDS             complement(13489..14877)
                     /gene="narU"
                     /locus_tag="ROI21_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDX5112173.1"
                     /translation="MALQNEKTSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFIPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            complement(15189..15881)
                     /locus_tag="ROI21_003287"
                     /pseudo
     CDS             complement(15189..15881)
                     /locus_tag="ROI21_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            complement(15869..16156)
                     /locus_tag="ROI21_003288"
                     /pseudo
     CDS             complement(15869..16156)
                     /locus_tag="ROI21_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            complement(16320..17420)
                     /gene="ompC"
                     /locus_tag="ROI21_003289"
                     /pseudo
     CDS             complement(16320..17420)
                     /gene="ompC"
                     /locus_tag="ROI21_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
     gene            complement(17679..18560)
                     /gene="yddG"
                     /locus_tag="ROI21_003290"
     CDS             complement(17679..18560)
                     /gene="yddG"
                     /locus_tag="ROI21_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDX5112174.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGCLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            18792..21839
                     /gene="fdnG"
                     /locus_tag="ROI21_003291"
     CDS             18792..21839
                     /gene="fdnG"
                     /locus_tag="ROI21_003291"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:19377..19379,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDX5112175.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWDEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADISGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            21852..22736
                     /gene="fdnH"
                     /locus_tag="ROI21_003292"
     CDS             21852..22736
                     /gene="fdnH"
                     /locus_tag="ROI21_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDX5112176.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            22729..23382
                     /gene="fdnI"
                     /locus_tag="ROI21_003293"
     CDS             22729..23382
                     /gene="fdnI"
                     /locus_tag="ROI21_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDX5112177.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
BASE COUNT         6028 a         5710 c         5860 g         5817 t
ORIGIN      
        1 gccagagttg tatcctgtat cagtcctgaa aaatttcatg agtgctttat taactggatg
       61 cttgactgcc attcttcaga tgataaagac gtcattgcaa ttgatggaaa aacgctccgg
      121 tactcttatg acaagagtcg tcgcaaggga gcgattcacg ttattagtgt gttctcaata
      181 atgcacagtc agattccttg gaagatcaag acgaatgaaa aaaacaatga gattacagct
      241 gtttctgaat ttcttaacat aatgggtatt aaaggaaaaa taattgcacc gatccaatgt
      301 gataccagaa agacatcgct aagaagatag ctatagacaa ttattaccct ccgtcagccc
      361 ttgttaagcg aggagagacg taatctgtcc agtcccctaa tgcttataaa gcccatcgat
      421 ttcgcccact ttctttcgcc cggtataacg cagcgtcggc atctgaaata atttgttcag
      481 ccgtttttgc gggagcagaa caggtaatcc cctgactaac cgtaacaaat gcattaacag
      541 cagatgcctg atgtgggata gctgccagtt taagttcttg cttcactctg gctgctaccg
      601 tctctgcttc cggcaatgtt gcattaaaaa gtatgatggc aaactcttcg ccaccataac
      661 ggacaacaac atcctccgac tggcgagttg aacgctgtag agcgtcggca acttgtatta
      721 gacaacgatc cccagcagga tgcccgtaag tgtcgttgta acgtttaaaa aaatcgacat
      781 ccagcataat aagagcaaat gacccgccgt tatccatcgc gtcgtgtaag atactgttca
      841 ttgcacgtcg gtttgctatc ccagttaaag gatcacgatg tgctaatgca tcaaggcgat
      901 taattaactg gttgttataa ctgttttgta tccatgcttc ttcaaaccaa cgtaacaaaa
      961 tgagtcgccc accatatagc gccagtgaaa aaatacacca tacaacagca aaatgaatat
     1021 tgatctgttg attaagcata agactcgcta ttggagtggt gaaccataag ggaatgacaa
     1081 atgcgagcaa tgatggggta tggaaataaa gtgcgacaac gccactaagc attaataata
     1141 cacatagtgg ataagcaaac tgcagcgtcc agtacgcaat aaagtgataa ctacaccatg
     1201 accatagcag gctcagggca actaatagca cctggatagg taatgttttt tgccgaggga
     1261 tgagtagtaa accactgcaa atcagcagaa tacccgttgc agatatatca atagaatgta
     1321 taaggctttt ttcatttaac cagggtggag taaggtcatt aaacagaaca cggcgcagca
     1381 aaattatcaa tgcaaaactg acattgataa aagcaaacca tggcaaacta atcgtcatgc
     1441 cgcgaataac catatcgcgg cgggttttcc atgagtgatg agtttttctt cgttcagcca
     1501 ttttttaacc aaaaaagggc aggatacctg cccggtatgc agtaaattta tggtgtcact
     1561 atattgaacc aaaattcaaa attatccata taacctaaca gctcgttgag tttttcctca
     1621 ttgccagtaa ttttgacatc tcctttggcc gtggcttctt tcagcgtctc ttctttcagt
     1681 acaattttgt tcaatacatc acgagacaga gtgatagtgg catcagcatc cgaagcctct
     1741 acaccagcgg tatgattgag aacaccattt tcaagctcca ctttataggt gccgccatct
     1801 tcaccaaaat cgaaattcaa cacggttttg gcatctgccg ctttctcacc attaatatgg
     1861 acggcaagat aatcaaagaa catctccggc gtcatcgcac gcacggtgtc tgggctggca
     1921 gtatttggtg ttggtaattt ttgtacgcca ttacgcaatt cctgtgcacc agtgagatag
     1981 aaattacgcc acggcccaga ttcagcctga tagcctaact gctcaagcgc atcagcttcc
     2041 agatctctcg ctgcctggtt gtttggatcg gcaaacacca ccttactaac aacttgggca
     2101 acccagcgga aattaccctg gtcataatcc tgtgtggctt tctgtaaaat ggcatcagca
     2161 ccgcccatat actcgacgaa tttttttgcg ccttcttctg gcggcagttc atcaagcgtc
     2221 gcggggttac catcaaacca gccgagataa agcacgtaag ttgctttcac gtcatgactt
     2281 accgaaccgt aatagccgcg attcgcccag gtgttagcca gtgaagaggg cagtttaaag
     2341 tttgcggcaa tttcatcacg ggtcatccct tgattagcca tgcgcagagt ttgatcattg
     2401 atgtaacgat acaaatcacg ctggctttta agcagtttga ccacgttatc gttgccccag
     2461 gttggccagt gatgttgagc gagaataatc tcggctttat caccccacaa attgagagct
     2521 tcattaatat attttgacca cggcagcggt tggcgaattt tagccccacg cagcgaatag
     2581 gtgttatgaa gggtgtgcgt gacatcttca gcggtttcga tcagtttctt ttcttcaata
     2641 aaccacagca tttcagaagg cgcttcagaa cctggcgcca tcaggaaatc ataagtcaac
     2701 ccgtcaatag tttctatttg tccggttttg gtgatgtagt tagtaggagc aatcaacgtg
     2761 actgttcctg ctgaagtagt cgttcccagt ccagccccca cctggccttt aacatcaggt
     2821 ttcaataagt tgccatacat ataactggct cgtcggctca taacattgcc agccatgatg
     2881 ttttcggaaa cggcttcctc caaaaatccg gctggcgcat agattttaac tttgcctgat
     2941 ttaacgtcag cttcatcaat tacgccacga acaccaccgt agtgatcgac atgactgtgt
     3001 gtatagataa ccgccactac cggttttttg ccgcgatttt tgtaatagag atccataccc
     3061 actttggctg tttctgctga aacaagggga tcaacgacgg taataccctc tttgccttca
     3121 atgatggtca tatttgatag atcgagattg cgaatctgat agacaccttc agttacttcg
     3181 aataacccgc tgatatttat cagttgcgcc tgacgccata aactcggatt caccgtatca
     3241 ggggctttat cgccttcttt gataaaagcg taatgttgtg gatcccagat aacattccct
     3301 tgttcgccct taatcacgtc ttgagggata ggggcaataa aacctttatg ggcatcagta
     3361 aagtcagtgt tatcggcaaa cggcaactgg ttgtagagta aattgtttgc ttgttgcgtg
     3421 gctgcggtgg cgtctttagg agcttccgcc gcgaggagag gtaaagaact ggtggtgaat
     3481 aatcccgtta ttagcagact tttaacgata tgattaagct gcatcttaag cgtctccaga
     3541 atggattttt attgggggta attacggatg aaaattttta gtgagggcaa gaataactat
     3601 aatgggcgtt aacaaattgg caagccggga aattgatgtt ttcttaatgg catgttggcg
     3661 aagaatatat tctgcaagtt aacgttttca ttggcgcttt atttaaaagt gtgtagtaca
     3721 aatatgtaca atttcaagta ggcattattg ccgtaaaaac gcattaccgg acacacgaca
     3781 ttcacaacgt gtgttaattt tactgacatc tttcaaaagg agcacaatca tgccgcacat
     3841 agacattaaa tgttttccgc gtgaactgga cgaacaacaa aaagcagcac ttgctgcaga
     3901 tattaccgac gttattattc gccatctgaa cagtaaagat agctcgataa gcatttctct
     3961 acagcagatt caaccagaat cttggcaagc tatctgggat gccgaaatcg cgccccaaat
     4021 ggaggctttg ataaagaaac ccggttacag catgtaatgc ttaacctgcc gtaacacgct
     4081 tgccgctggt aacaaacgtg ccagcaccta aatgatgcaa cgtattgagt ttgtcatcat
     4141 caaactgcca gcggccttcg acaaatactc gtgcgtctgc cgctgcagat accacttcgc
     4201 caaatagcgt atcgtatttt tcttgcgcgg aagttgcagg tagcaggcga cactccatcc
     4261 acgccagaca tttttcctcg accagcggca aaccaagcac cggacctttc acaaccggaa
     4321 taccatagca attaaactta tcttcatcac gccccgacac acttcccacc gcccacgtcc
     4381 agttagttgc tgcaacaccc gggataacga tgccaaactt accattacgt tcaatcaact
     4441 ccctggtcca tgtcgattta tctaccacaa tcgccacgcg tggcggttca aactccaccg
     4501 gcattgacca ggcggctgcc ataatgttac gccgctggga ttgctcatcg aaactggtga
     4561 tcaagacagt tgggccatga tttaacagac ggctggcatg gtgtaattcg acagggatga
     4621 atcggctcac ggtatcgcct cgctatgaag ggtgttttaa aagcgtagct cgtaacgcaa
     4681 taagtaacga aattaacggg attggcgatt tgcgaacgtg atgcatgtcc gcgatcgcac
     4741 aaaatagccg gtgcagcgtc tattccaggt tataagttga gaaaaccact aagggaaacg
     4801 cctgatgacg cccattctga atcactattt tgcccgtatt aactggttgg gagctgctgc
     4861 ggtcaatata gatacgctgc gtgcattgca cctgaaacac aattgcacca ttccgtttga
     4921 aaacctcgac gttttgctgc cgagggaaat acagcttgat gatcaatcgc tggaagagaa
     4981 actggtgaca gcccgtcgtg gtggttactg ttttgagcag aatggcgtgt ttgagcgggt
     5041 gttacgcgag ctggggttta acgttcgcag cttgttaggg cgcgtagtgt tatcaaatcc
     5101 gccagcatta ccgccgcgca cccatcgttt gttgttggtg gaagtggaag gggaaaaatg
     5161 gattgctgat gtcggtttcg gtggacagac gctaaccgcg ccgattcgat tagttcccga
     5221 gctcgtgcag accacgccac acggagagta tcggttgttg caggagggtg atgattgggt
     5281 gttgcagttt aatcatcatc agcactggca gtcgatgtac cgttttgatc tctgcgagca
     5341 gcaacaaagc gattatgtga tgggtaattt ctggtcggcg cactggccgc aatcgcattt
     5401 tcgccatcat ttgttaatgt gccgccattt gccggacggt ggcaagctga cactgaccaa
     5461 ttttcatttc acccattatg aaaatggaca tgcagtggag cagctaaatc taccggatgt
     5521 ggtgtcgttg tatgcggtga tgcaggagca gtttggactg ggcgtggatg atgccaaaca
     5581 tgggtttaca gtggatgatt tagcgttggt gatggcagcg tttgatacgc atccggaggc
     5641 ggggaaatag tttatatcag gttgccggat gcggcgtaaa cgccttatcc ggcctacatt
     5701 agccaatata ttcaataaat tacgatgcgg ctgtaggcct gataagcgaa gcgcatcagg
     5761 caacggtttc agagttcaat tgcccattcg gcatgaaaca aaatcaccgc cgtgccggaa
     5821 atggtaactt tctccggctg gttatcgcga attgtcaccg ttacatcaat cactccatca
     5881 cgacccaatg cgcgcccctg atggccttta acacgcaaca cgttgccatc gtggggcaat
     5941 acgttgtgat gtactaacca tgcgcccatc gggccattgg cattgccggt caccggatct
     6001 tccacgatac caatcgcagg cgagaacatg cgaccatcgg tttcattttt gccagggcgg
     6061 atctggaacg ggaagaaacc attgcagcca atctgtttac tgatagcggt cagcgcagca
     6121 aggtcaggcg aaagggcgtc gatatccact tctggtttca gcggaatcat cacttttgag
     6181 tgtcctgtgg ttgccacctg gattggcaag cctggcaaaa tatcgtcttc agtgagatga
     6241 agcgcgttga taatcgccgc acgtgtttcg ccttccagcg gtggctcaaa gcccggcgta
     6301 ccttgttcca gcgaaatacg ataatcatcg ttttgctttt caacagtcac gcgatgccgt
     6361 cctgccagag atgtttgcca gaccgtgcaa tttcccaaac ctaatacctt cgaacgtaca
     6421 tagtgcgcag ctactgtcgc gtgaccacaa atcggcactt caaccgttgg cgtaaagtag
     6481 cggatgcgca catcgctgtc gtcgctgtgc agcagaaaag cagtttcgga atgacctaat
     6541 tcgcgggcga taagctgcat ttgcgcttcg ctgagattat cggcggggaa aacaacaccg
     6601 gcagaattgc cgcgaaatgg ttgagaggta aaagcatcga cgtggtaaac ctgcggtttc
     6661 attaacgttc tcctgtgact ggagaactat catagcctgc aagtggccgg agagcgaagg
     6721 gctatccggc cagggtgaaa ttatcgccgc gagcgcacaa tctgataccg acgagtaaag
     6781 tactcaaacg gcgcgctcca cacatgcacc agtcgggtga acgggaagag caggaagatg
     6841 gtcattccca acaccagatg caggcggaaa acgaacgcta cgccgttaag catttctgac
     6901 gatccaccac ggaaagtgac aatgctttgc gcccaaccca ccagcttcat catttcgcta
     6961 ccatcaggat actgtgctga aaacggtatg gtacttagcc ccagcagaca ctgaatcagc
     7021 aaaatgatca tgatgataat atccggcgta gtggaagtgg cacgcacgcg ctgatttgtc
     7081 agcctgcgcc acagcagtcc tgcgccgcca atcagcgtca atacaccgca aataccaccg
     7141 agcaccatcg ccatctgctg ttttaccgcc accggcagaa accacgcgta catccagtgc
     7201 ggcgttaaca tgccgaacag gtgcccgaag aaaatcccta aaatgccgat atggaacaga
     7261 ttcgaccata tcaccatccc gcgtttatcg agcatctgac tcgaggaggc gcgccaggta
     7321 tactgcccgt agtcgtagcg cagccagctg ccgaggaaaa acactgtcgc acaaatgtac
     7381 gggtagatat cataaaaaaa gacgttcaga tactgaatca tttcccacct cccgcactga
     7441 tgtcgacata ctgcggcgcg acatcctggc taaagcgtcg ctgatattga ttaagcggcg
     7501 agctgtcaca cgccgtggcg ttatcttcaa taaacttcac ctgttcctct tcccacaccg
     7561 catccagtgc ctggcgggtg tcatcgcgct cctcactgtt cacctgtttt gtgacactgt
     7621 cacttgaaag tgtacttcct gccagttgca acagggtgtc aaataacgcg taccacggtg
     7681 cctcgcgttg ttttaagcgt ccgccaagca gggcgaggat cggcgcaacg ttgagcaagc
     7741 cttctttcgc ctgatcgtcc ggcagtacgc ttaagtactc cagatacagc ggtagataat
     7801 cgggcagttc tcgacaatcc aactgcaggc cgaccttttc atactccgcc agcaggtcca
     7861 ccattgcctg gccgcgatcg cgggactcgg catgaacatg ttcgaacagc agcagcgatg
     7921 tggtacgtcc acggtcaaac ccttcgcacc attcggcctg tttatccagc agcggcgcgt
     7981 taagcaggtt gcgggtaaaa tccgtaagca tcggtgcgtc gcggcggatc aacgccagcg
     8041 cgtcctcctt acattcccat aacagctcgt ccggatactc catcaacagg ccgatcactt
     8101 tgaggatctg cattattcgc cctccgcttt gtcgcgtact tcggtgatgt tgatggcatc
     8161 gatacggcta ctgttgaaca ggttgaattt gctgtccgag ccgtggcaac cgtcgccaaa
     8221 ggtaaaaccg cagccattgc gttctgcgaa ggcatcgccc gccatttccc gatgactggt
     8281 tggaatgaca aaacggtctt catagttggc aatggcgagg tagcgataca tctcttcgac
     8341 ctgggcgacg ctcaggccta cttcgtcaat ggcgcgggta tcggtaacgc cttccacggt
     8401 ttgtgaacgc atatagtggc gcatcgccat catccgtttt agcgcccgca gtaccggacc
     8461 ggtatcgccg gcacttaaca tattggcgag atattgcaca ggaatacgca ggctctcgat
     8521 ggctgggagc acgccttcgc ttttcggcaa accgcccgca tctgcgtagg actgaatcgg
     8581 tgacagcgga ggaacgtacc agaccatcgg cagggtgcgg tattcagggt gcaacggtag
     8641 cgccagtttc cagtccatcg ccattttgta gacaggcgaa cgctgggcag cgtcaatcac
     8701 attttgtgga ataccttgtt tcagggcttc ctcgatcact gagggatcgt gtggatcgag
     8761 gaatacttcg cactggcgtt catagaggtc aacttcgcgc tcggtgctcg ccgcttcctc
     8821 gatgcggtca gcgtcgtaaa gcagcacgcc cagataccgg atgcgaccca cgcaggtttc
     8881 tgagcacacg gtgggttggc ctgactcaat tcgtgggtaa cagaagatac atttttctga
     8941 cttgccgctt ttccagttga agtagatttt tttgtacgga caaccgctaa tgcacaaacg
     9001 ccagccacgg catttatcct gatcgatcag cacaatgccg tcttcttcgc gtttgtagat
     9061 agcgccgctt gggcaggttg ccacgcaact gggattgagg cagtgttcgc acaggcgcgg
     9121 caggtacatc atgaaggtgt tttcaaactg cccgtacatc tccttttgca tggcctcgaa
     9181 gttgcggtca cgggcgcgtt tttcgaattc gccgcccaac aattcttccc agtttgcccc
     9241 ccagatcact ttgtccatcc gtttgccgtc aatcagtgaa cgcgggcgag cagtagggat
     9301 atgtttgcct tccggtgcgc tatgcaaatg ttcgtagtcg aaggtgaaag gttcgtagta
     9361 atcatcaatc tgcggcacca ccgggttggc gaagattttg gttattacgc ccatcttgct
     9421 gcccagacgc gggcggatct tgccattcac atcacgcacc cagccgcctt gccactcttc
     9481 ctgatcttcc cagtttttcg gataaccaat gcccggtttg gtttcgacgt tgttaaacca
     9541 tgcgtactcc atgccttcgc gtccggtcca gacgttttta caggtcaccg aacaggtatg
     9601 gcaaccgata catttatcga ggttaagcac catgccgact tgcgaacgta ttttcatttt
     9661 ttcgcctcct gtacctgatc gcgaccttca tcatccagcc agttaacgtt cttcatcttg
     9721 cggatcatga tgaattcgtc acggttcgag ccgacggtgc cgtagtaatt aaagccccag
     9781 gccagctgcg cgtaaccgcc aatcatatgc gttggtttcg gacaaacgcg ggtgaccgag
     9841 ttatgaatgc cgccgcgcat tccggtcact tccgagcccg ggatattcat aatgcgttcc
     9901 tgagcgtgat acatcatggt catacccggc ggtacacgtt ggctgaccac cgcgcgggca
     9961 gtcagcgcgc cgttggcgtt aaacacttcc acccagtcgt tatcgacaat ggtcagttca
    10021 cgggcatcgg tttcgctaat ccagacaatt ggtccaccgc gagagagcgt cagcattagc
    10081 aggttttcac tgtaggttga gtgaatgccc catttctggt gcggcgtcag gaagttaagt
    10141 gctttttccg ggaagccgtt tggcggtatc tggcgcattt cactgacgct acgggtgtcg
    10201 ataggcgggc gataagccac caggctttca ccaaaagcac gcatccacgg atgatcctga
    10261 tagagctgct ggcgtcccga cagcgtgcgc cacggaatta actcatgaac gttggtgtat
    10321 cccgcgttat aggaaacgtg atcgctttcc agaccggacc atgtggggct ggagataatt
    10381 ttacgtggct gcgcctgaat atcgcgaaag cgaatcttct cgtcctcttt gtgcagcgcc
    10441 agatgggtat gttcgcgtcc ggtgatctcg cccagtgcct gccacgcttt gactgcaaca
    10501 tgaccgttgg tttccggtgc cagcgccaga atcacttctg atgcgtcaat ggcggtgtca
    10561 atcagcggac gcccctgtgc cgggccttca cgcttggtgt agttgagttt gccgaggaaa
    10621 tcgatttcat cctgggtatt ccacgaaatc cctttaccgc cgttgccaag tttgtccatc
    10681 aatggcccga gcgaggtaaa gcgttcatag gtagcagggt agtcgcgttc caccgccaca
    10741 atattgggcg cagttttacc tggaatcaga tcgcattcgc ctttgcgcca gtcgagcact
    10801 tcacacggct gtgagagttc tgccggagag tcatgcagca gtggttgtaa taccacgtcg
    10861 gtttctttgc caagatggcc cacgcacact tgcgaaaagg ctttggcaat acctttgtag
    10921 atttcccagt cgctgcgtga ttcccacgcc ggatcgaccg ccgcagatag gggatgaata
    10981 aacggatgca tatccgaggt gttcatatcg tctttttcgt accaggtggc ggtgggcaga
    11041 acgatatcgg agaacaggca ggtactggac atacggaagt cgagcgtcac cagcaggtcg
    11101 agcttgcctt caatcgccgc agtttgccac tcgacttctt ccggtttgat cccgtcgctg
    11161 gcaccgagtt cctgaccctg aatcccactt tcggttccca gcagatactt ctgcatatac
    11221 tcgtggcctt tgccggagga gccaagcagg ttagaacgcc agacaaacaa attacgcgga
    11281 tgattgctgc cgctatctgg ttgttcgcag gccatgcgta aatcgcccga tttcagcgcc
    11341 tgggcggtaa attctgtggg ggataatccg gccttgtcgg cttcagcttt aatcccgagc
    11401 gggttacgcc ccagctgcgg cgcagaaggt agccagccca tacgttcggc acgaacgttg
    11461 aaatcaatca gatgaccgct gtacttactg gcatcggcga gcggtgaaag taactcctga
    11521 gcagagactt tttcatagcg ccattggctg gaatgattgt agaaaaacga ggtgctgttc
    11581 atctggcgcg gtgggcggtt ccagtcgagc gcaaaggcca gcggcaacca gccggtttgt
    11641 gggcgcagtt tttcctgacc gacatagtgt gcccatccgc caccgctttg cccgacacaa
    11701 ccgcagaaga tcagcatgtt gatcatccca cggtagttca tgtccatgtg gtaccagtgg
    11761 ttaacacctg cgccgaggat aatcatcgag cgcccatgcg ttttatgggc agtatcggca
    11821 aattcacgag cgatggtttc aatatactgg cgcggcacac cggtaatttg ctcaccccag
    11881 gctggggtgt acggtttgat ttcagcgtaa tctttcgcgc tgttttcatc ttccagcccg
    11941 cgatcgaggc cgtaattcgc cagtaccaaa tcataaacgc tgaccaccgg acaggtgctg
    12001 ccatcggcta atgtcaggtt tttaacgggt aattgacgca ccaggacagg attgtttttt
    12061 acgctgcgaa aatgcggatt ttcaatgccg ccaaagtagg ggaaggccac gcctgcaacg
    12121 gcatcatgtt gaccgagcag agttaacgac aattcggttt ccgtaccggc ggcaatggat
    12181 tcaagattcc atttgccttt ttctccccag cgaaaaccaa tcgaaccgtt cggcaccacc
    12241 agttcacctg cggtattaac cgccacggtt ttccactgcg gattgttgct ttcgcccagt
    12301 ccattcacca ggtcagatgc gcggaccatg cgcccgggaa cgtagctgcc gtcgtcgcga
    12361 ggttccagca ttaccagcat cggcatgtcg ctgtagcggc ggcagtagtt gataaaataa
    12421 tcgctgggat tatcgagatg aaactctttt aaaatgacat ggcccattgc catcgccagg
    12481 gcgctatcag tgccttgttt cggtgccagc cactggtcgc acaatttggc cacttcagag
    12541 tagtcagggg taatggcgat ggttttagtg cctttgtagc gtacttcggt aaagaagtgg
    12601 gcgtccggcg tacgtgtctg aggtacgtta gacccccagg cgatgatgta gctggagtta
    12661 taccagtcgg cagattctgg cacgtcggtt tgctcgcccc aggtcatcgg cgaggcaggc
    12721 ggcaggtcgc aataccagtc atagaaactt aaacaggtgc cacctaacag cgacagataa
    12781 cgtgttccgg cggcgtaaga aaccatcgac atcgccggga tcggcgagaa accggcaacg
    12841 cgatccgggc cgtaagtttt gatggtccag acgttagcgg cagcaatcag ctgatttagc
    12901 tcctgccagt tagagcggat aaagccgccg cgtccacgta cttgtttgta gctcaggcac
    12961 ttttgcggat cgttcataat cgatgcccac gccagtaccg gatcgctgtg ttgcttgagg
    13021 gcttcgcgcc acagttcaat cagtcgttta cgaatgagcg ggtatttcag acggttagcg
    13081 ctgtagagat accaggagta acttgcgcca cgcgggcagc cgcgaggttc atgattgggc
    13141 aggtcagggc gagtgcgcgg gtagtcggtc tgttggattt cccaggtcac cagaccattt
    13201 ttaacgtaga ttttccagct acaggagcct gtacagttaa caccgtgggt ggaacgcacg
    13261 attttgtcga actgccaacg ctggcgatag ctgtcctccc agtcgcggtt gctatgcatc
    13321 acctgtccgt gaccatcggc aaaggtttcg cccttttgtt tgaagtagcg aaagcgatcc
    13381 aaaagtttac tcatgacata actcctgctc caggaatgaa ttgggatctg atgccttgtc
    13441 ggatgcgaca cggttgtagc ctgataagac gtgtctggca tcgatctctt atttttggct
    13501 gaacttccgc cgaccataaa ccagccaggt cagcagcaca caaacgatgt agaagattaa
    13561 aaacactttc atcgcgccta ccggagagcc ggtcatattg agcgacatgc caaacgcctg
    13621 cggaatgaaa aagccgccca ctgcgccaat ggctgagata aagcccagag ccgccgccgt
    13681 ttcggtgacg gcttctctct gagcttgctc atcactaccg cctttcatct tcacccgata
    13741 aatggttatc tggcgaaaga tgacggcgat catctggaaa gtagaaccac ttcccagacc
    13801 tgcggtcaga aacaacccca taaatacggc gtaaaatgcg atgaaattac cggagcctgt
    13861 gcccggtaag gtaaggaaca gcagggcgct gaaaatcgcc ataaaaatga agttgatcaa
    13921 cgtcacccgc acgccgccga atttatcgga aatagcacca ccaaccgacc gcgcgatggc
    13981 accaataaaa gggccaaaga acgccaggcg cagaatattc acatcaggga actgagtttt
    14041 cgccagcatg gcaaaacccg cagaaaaacc gataaacgaa ccgaaggtgg caaggtaaag
    14101 caggctcagc agccagagat gcaggcgttg taacactggc agctggtcgg caattgacgc
    14161 gcgtgaactg gcgatatcat tcatccctga ccaggcggcg atcgtggcaa tcgccagtaa
    14221 tggcacccaa atccatgcgg cattcgccag cgacatcacc gaaccgtcgg cctgcggtac
    14281 gccattgacg ccgagaaagg caaatacggg tataaaaatg accagcggtg caaccagctg
    14341 catcacactt acacctaagt ttcctaatcc gccattaatc ccaagtgcgc tcccttgttt
    14401 ggcttttggg aagaagaaac tgatattgcc catgctcgaa gcaaagttcg cacctgcaaa
    14461 accgcatagc aaagcgataa cgataaatat cccaaaagga gtattcggat tctgcacggc
    14521 aattccgagc cagacgcaag gaataatcag gattgcagta ctaaaaaccg tccatcggcg
    14581 tccaccaaat ataggcacca taaaggagta gggaacacgc aataatgcgc cagaaacgga
    14641 gggtaatgcg gttaataaaa agagttgatc ggtagtgaaa ttaaaaccga ttttattgag
    14701 attaacagta actgcgctaa atagcatcca gacacagaag gcaagaagta gacaactgac
    14761 tgatatccag aggtttcttc gtgcaatatg ctttccttta ttttcccaga aggccggatt
    14821 ttctggtttc cagtcgcgca aaagataacg actagttttc tcattttgca gtgccatatt
    14881 gttcctcaca tgtacacatt ggtaatgaaa aaaagacaaa acaggaggta aggcgcaata
    14941 gccagttatt agaattaagg atgaattggg tgaagtgctg attgaaagaa tagataagaa
    15001 agcgtaaccg cgggggcaga atggggatta aacagacaga tatgtgttac taaatgtaac
    15061 taacaagcta accattaaat ggatgttaag ctatgtatgc atgtcatgat ccaacccttc
    15121 agatgatgaa tccaaagggt ttatattttt aacaagacca gacacttata gaattagtga
    15181 tgatttattc actgaaaagg ccatgagctg gaggaaaacg atctgtccag aaagtctatc
    15241 ggttcctctc ttatcgcctc attgtaataa tcgttattac agtaagtatt gtttatttca
    15301 tctaatgtca tatttttaat ttttatattc atctgattta caacttcttc tgttttataa
    15361 gtgtgtatat tatatggggt atatgcttct ttaatagtat agtgaaacaa ttcgttatcc
    15421 acatctactc cttcagagtg aatttcaaat gcaccaggcg aaagaattgc caggccagta
    15481 ttaagcttaa gtgctgggga aaataaatta aataacatga gctgcatagt agggcaattg
    15541 ttcttcccag aaacatttaa aaaatttaag tttttgctct gttttattat ggggaataaa
    15601 gttccaatat cggacgtatt cttagtgtca atgcgggtta ttttattatt gtccagcctt
    15661 aaacttttga tagatgaaag ataagtcagg aaatgaaggt ttattttttc caactgattg
    15721 ttcgcagcgt ttaatgtctg aatattgcag aagttgctaa agaatatttt aatttcagaa
    15781 agcaagttgt tgttgataag taacgtattc aagttgggaa ataaggtatt atgtaaactt
    15841 gttagtttat tatgggatag atccagaaga gacaattgtt ttccagattt aacgtgttaa
    15901 ctgtcgaggg gataggcggt aactcactca aatcaagata tgacaaatca agagatgcca
    15961 tgttcgaatt gtaagcttct ttaattcgct ctacggcaat tgctctttct tcaccattat
    16021 taagcgactt attaccccat tgtgccagtc gttcacaaaa attgctggag atggtttctt
    16081 tgttaggtaa tgcgttattt aattgataac tggcattatt ttttacacaa gctgtattaa
    16141 tattggtcat aatcactcat caataaatat tatagtaaga tgtccccgga gggtatggaa
    16201 gaatattaat tgaggtgaat gaccagaaat atcaaaaatt gctcattttt acttaaggag
    16261 tcatacagca gcacttaaat caaaaaccag cccagagaag ggctggtcag gagaggatgt
    16321 tagaactgat aggtaatgcc cgttgcgacg atgttatcgg tgcgaatatc tacggcacgg
    16381 gtaaagtcgc tttcatcaat caggttgatt ttataatcaa cataggtcga catatttttg
    16441 ttgaagaaat acgtcgcacc aacgtcgata tactcaatca tgtcctggtc gccgtagcgg
    16501 cccagatctc tgcctttcga tttcaggtaa gcgagtgatg ggcgcaagcc gaagtcgaat
    16561 tgatattgtg ccactacttc aaaactttgt gctttgttag agatataacc atcgccatag
    16621 gtggtcatgt ttaatgtttc agcgtaatta gctgcaaagt agatgttgtt ggcatcatat
    16681 tttatacctg tagcccacag ttctgcattc ttaccggagg cattgagagg attagcagcg
    16741 gctctttcct gcgcatttgt ccgatcggat ttggtataca ccgcaccgat accaaagcca
    16801 tcataaacat aagttgagga gataccgaaa ccgtcaccat tggcacgcgt gtagtcagca
    16861 ccataaagat gaccattgtc agtgcgttca tttttgcctt gatattgcgc ggcaaagttc
    16921 agaccttcaa ttaagccgaa gaaatcgtag ttgcgatagg ttgccacacc ggtagtacgg
    16981 tatgtcatga agacatcttt ctgactccag gtatcgccac caaattctgg caacatatcg
    17041 gtccatgaac caatgtcata gccgacaccg tagttacgac catagtccag agagccaaat
    17101 tcactaattt ttaaacctgc ataggccaga cgggtcttgt taccgcgcga gccgttggct
    17161 tcatcgttat agcctttaaa atcatattac cagtgaccaa aaccaatcat ttgatcattg
    17221 atttgcgttt ctcctttaaa gccgagacgg gcataagttt tatcaccatc gtcacgctta
    17281 tcatcagtaa aataacgtag agcggtaacc ttgccataaa ggtcgagttt attaccatcc
    17341 ttgttataga cttcggcagc gtgtgctacg ttcgcagcta acaaaccagt tacaaccacc
    17401 gcaactattt ttaatttcat ttaacagaat ccttttaatt atcgttaaac gtattttcta
    17461 aacgaatttt aaacggcgtc atttataaat gacatactgt ttttaaaata gaatggtgat
    17521 taaaaacaat gagaaaaata gatgaaataa tattatttat cgatatgtga tcgaagtcga
    17581 aatgaaatat aaggtgaact actggtattt gaaatttatt tttttatatt gtcggaattt
    17641 atctgattaa ctgccgggcc gtagacccga caattatttt agccacgacg tgtcgccagc
    17701 cagcagagca gggaaccgcc gcagaccatt agcgcgcctt gccagaacga gaacgacagc
    17761 ggggcgctga gcagcacggc tgcaagcgct gaggaaagga cgggcgtaaa atacgaacct
    17821 accgccataa tggtgacgtt gccatgtaat ataccgacgt tccatgcagc ataagcaaat
    17881 cctaaggtaa atgccgcaga gatgagttta atcatgactg gcgtgctaaa taccatttct
    17941 ggttgtggcg tgagaaaata gtaaacccac agacttgctc ccgttagcag gacaaaaacg
    18001 gtaattccgt taaatccgcg tgcgtattta ttcgttactg tgcagtaggc tgcccagata
    18061 aacgcaccaa taaacgccag gaaataactc aatgggctgg tggtgatatt attgatgatt
    18121 tcatcataat gtaacccatt gtcaccacct aacacccagc agacgccaac cagggctaat
    18181 aataatcctg gtacaatcag ccagttggtt ttctgaccat taaacagaat ggcaaagaga
    18241 attgtcaggc tgggccacag atagttcacc atacccactt caatcgcctg atgacgggtc
    18301 gccgcatatc ctaaggaaag cgccagacag atttcatagc tgacgaataa cagacacccg
    18361 gcgagtaaat agcctttcgg aatctgccga atacgcggaa atccaaccgt gaagattaac
    18421 agcagcccgc ttaatgaata gatagcagcc gcgccgccga ccgggccgag cccctcactg
    18481 acaccgcgaa tcaatcctac catcgtgctc cacaggacga tcgctattag ccctatgagc
    18541 gttgcttttt gtcgtgtcat gctcgctgtt ttgtctctct tgccgttaaa aattaagctg
    18601 aattttatag cattttttta actggcctgt caggcagtgg tgcgtttttc taccgctatt
    18661 gaggtaggtc aatttgcgca ggcggattat tttgtggcaa acacgtgttc tttttgattt
    18721 cgcgcaaaaa gattcagaat tttactgtta gtttcctcgc gcagtaatac ccctgaaaaa
    18781 agaggaaagc aatggacgtc agtcgcagac aattttttaa aatctgcgcg ggcggtatgg
    18841 cgggaacaac agtagcagca ttgggctttg ccccgaagca agcactggct caggcgcgaa
    18901 actataaatt attacgcgct aaagagatcc gtaacacctg cacatactgt tccgtaggtt
    18961 gcgggctatt gatgtatagc ctgggtgatg gcgcgaaaaa cgccagagaa gcgatttatc
    19021 acattgaagg tgacccggat catccggtaa gccgtggtgc gttatgcccg aaaggggccg
    19081 gtttgctgga ttacgtcaac agcgaaaacc gtctgcgcta cccggaatat cgtgcgccag
    19141 gttctgacaa atggcagcgc attagctggg atgaagcatt ctcccgtatt gcgaagctga
    19201 tgaaagctga ccgtgacgct aactttattg aaaagaacga gcagggcgta acggtaaacc
    19261 gttggctttc taccggtatg ctgtgtgcct ccggtgcaag caacgaaacc gggatgctga
    19321 cccagaaatt tgcccgctcc ctcgggatgc tggcggtaga caaccaggcg cgcgtctgac
    19381 acggaccaac ggtagcaagt cttgctccaa catttggtcg cggtgcgatg accaaccact
    19441 gggtggatat caaaaacgct aacgtcgtaa tggtgatggg cggtaacgct gctgaagcgc
    19501 atcccgtcgg tttccgctgg gcgatggaag cgaaaaacaa caacgatgca accttgatcg
    19561 ttgtcgatcc ccgttttacg cgtaccgctt ctgtagcgga tatttacgca cctattcgtt
    19621 ccggtacgga cattacgttc ctgtctggcg ttttgcgcta cctgatcgaa aacaacaaaa
    19681 tcaatgccga atacgttaag cattacacca atgccagcct gctggtccgt gatgattttg
    19741 ctttcgaaga cggtctgttc agcggctacg acgctgaaaa acgccagtac gataagtcgt
    19801 cctggaacta tcagttcgat gaaaacggct atgcgaaacg cgatgacacg ctgactcatc
    19861 cgcgctgtgt gtggaacctg ctgaaagcgc acgtttcccg ctacacgccg gacgtagtag
    19921 aaaacatctg tggtacgcca aaagccgact tcctgaaagt gtgtgaagtg ctggcctcca
    19981 ccagcgcgcc ggatcgcaca accaccttcc tgtacgcgct gggctggacg cagcacaccg
    20041 tgggtgcgca gaacatccgt actatggcga tgatccagtt actgctcggt aacatgggta
    20101 tggccggtgg cggcgtgaac gcattgcgtg gtcactccaa cattcagggc ctgaccgact
    20161 taggcctgct ctctaccagc ctgccgggtt atctgacgct gccgtcagaa aaacaggttg
    20221 atttgcagtc gtatctggaa gcgaacacgc cgaaagcgac gctggctgat caggtgaact
    20281 actggagcaa ctatccgaag ttcttcgtta gcctgatgaa atctttctac ggcgatgccg
    20341 cgcagaaaga gaataactgg ggctacgact ggctgcctaa gtgggaccag acctacgacg
    20401 tcatcaagta tttcaacatg atggatgaag gcaaagtcac cggttatttc tgccagggct
    20461 ttaacccggt tgcgtccttc ccggacaaaa acaaagtggt gagctgcctg agcaagctga
    20521 agtacatggt ggttatcgat ccgctggtga ctgaaacttc taccttctgg cagaaccacg
    20581 gcgagtcgaa cgatgtcgat ccggcgtcta ttcagactga agtattccgt ctgccttcga
    20641 cctgctttgc tgaagaagat ggttctatcg ctaactccgg tcgctggttg cagtggcact
    20701 ggaaaggtca ggacgcgccg ggcgaagcgc gtaacgacgg cgaaattctg gcgggtatct
    20761 accatcatct gcgcgagttg taccaggcag aaggtggtaa aggcgtagaa ccgctgatga
    20821 agatgagctg gaactacaag cagccgcacg aaccgcaatc tgacgaagta gctaaagaga
    20881 acaacggcta cgcgctggaa gatctctatg atgccaatgg cgtgctgatt gcgaagaaag
    20941 gtcagttgct gagtagcttt gcgcatctgc gtgatgacgg tacaaccgca tcgtcttgct
    21001 ggatctacac cggtagctgg acagagcagg gcaaccagat ggctaaccgc gataactccg
    21061 acccgtccgg tctggggaat acgctgggat gggcctgggc gtggccgctc aaccgtcgcg
    21121 tactctacaa ccgtgcttcg gcggatatca gcggtaaacc gtgggatccg aaacggatgc
    21181 tgatccagtg gaacggcagc aagtggacgg gtaacgatat tcctgacttc ggcaatgccg
    21241 caccgggtac gccaaccggg ccgtttatca tgcagccgga agggatggga cgcctgtttg
    21301 ccatcaacaa aatggcggaa ggtccgttcc cggaacacta cgagccaatc gaaacgccgc
    21361 tgggcactaa cccgctgcat ccgaacgtgg tgtctaaccc ggtggttcgt ctgtatgaac
    21421 aagacgcgct gcggatgggt aaaaaagagc agttcccgta tgtgggtacg acctatcgtc
    21481 tgaccgagca cttccacacc tggaccaagc acgcgttgct caacgcaatt gctcagccgg
    21541 aacagtttgt ggaaatcagc gaaacgctgg cggcggcgaa aggcattaat aatggcgatc
    21601 gtgtcactgt ctcaagcaag cgtggcttta tccgcgcggt ggcggtggta acgcgccgtc
    21661 tgaaaccgct gaatgtaaac ggtcagcagg ttgaaacggt gggtattcca atccactggg
    21721 gctttgaggg tgtcgcgcgt aaaggttata tcgctaacac tctgacgccg aatgtcggtg
    21781 atgcaaactc gcaaacgccg gaatataaag cgttcttagt caacattgag aaggcgtaag
    21841 ggggcgaaca gatggctatg gaaacgcagg acattatcaa aaggtccgca actaactcca
    21901 tcacgccgcc ttctcaggtg cgtgattaca aagcagaagt cgcgaaactt atcgacgttt
    21961 ccacctgtat cggctgtaaa gcctgtcagg tggcgtgttc agagtggaac gacatccgtg
    22021 atgaagtggg gcactgcgtc ggggtttacg ataaccccgc cgatctgagc gccaagtcct
    22081 ggacggtgat gcgctttagc gaaaccgaac agaacggcaa gctggagtgg ctgatccgta
    22141 aagacggctg tatgcactgt gaagatccgg gctgcctgaa ggcgtgcccg tctgctggtg
    22201 caatcattca gtacgctaac gggattgttg atttccagtc ggaaaactgc atcggctgtg
    22261 gttactgcat tgccgggtgt ccgtttaata ttccgcgcct caacaaagag gataaccggg
    22321 tatataaatg cacgctctgc gtcgatcgcg tcagcgtcgg ccaggaaccg gcttgtgtga
    22381 aaacctgtcc gaccggggct atccacttcg gcaccaagaa ggagatgctg gagctggcgg
    22441 aacagcgcgt ggcgaaactg aaagcgcgtg gttacgaaca tgctggcgtc tacaacccgg
    22501 aaggggtcgg tggtacgcac gttatgtacg tgctgcatca cgccgatcag ccggagctgt
    22561 atcacggtct gccgaaagat ccgaagatcg acacctcggt aagcctgtgg aaaggcgcgt
    22621 tgaaaccgct ggcagcggct ggctttattg ccacttttgc cgggttgatt ttccactaca
    22681 tcggtattgg cccgaataag gaagtggacg atgacgagga ggatcatcat gagtaagtcg
    22741 aaaatgattg tgcgcaccaa atttattgat cgcgcctgtc actggaccgt ggtgatttgc
    22801 ttcttcctgg tggcgctgtc cgggatttcg ttcttcttcc cgacgctgca atggctgacg
    22861 caaaccttcg gtacgccgca gatggggcgc attttgcacc cgttcttcgg cattgcgatt
    22921 ttcgtcgcgc tgatgtttat gtttgtgcgt tttgtgcatc ataacatccc ggataagaaa
    22981 gatattccgt ggctgttgaa cattgtcgaa gtattgaaag gcaatgagca taaagtagcg
    23041 gatgtcggta agtacaacgc cgggcaaaag atgatgttct ggtcaatcat gagcatgatt
    23101 ttcgtgctgc tggtgaccgg ggtgattatc tggcgtccgt actttgcgca gtacttcccg
    23161 atacaggttg tccgttatag cctgctgatc cacgcggctg cgggtatcat cctgatccac
    23221 gccatcctga tccatatgta tatggcattc tgggtgaaag gatcgattaa agggatgatc
    23281 gaagggaagg tgagccgtcg ctgggcgaag aaacaccatc cgcgctggta tcgtgaaatc
    23341 gagaaggcgg aagcgaaaaa agagagtgaa gaagggatat aatctctttt gaactttaag
    23401 ctgaaaatgg cgctt
//
LOCUS       DAPOZO010000077        23213 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPOZO010000077 DAPOZO010000000
VERSION     DAPOZO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.077"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..962)
                     /locus_tag="ROI21_003294"
                     /pseudo
     CDS             complement(1..962)
                     /locus_tag="ROI21_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            complement(1018..2142)
                     /gene="yeaW"
                     /locus_tag="ROI21_003295"
     CDS             complement(1018..2142)
                     /gene="yeaW"
                     /locus_tag="ROI21_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDX5112178.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLNGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTLTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(2174..3784)
                     /gene="yeaV"
                     /locus_tag="ROI21_003296"
     CDS             complement(2174..3784)
                     /gene="yeaV"
                     /locus_tag="ROI21_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDX5112179.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLKEGDDPDRALRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EDYLPQTPVEASEKNPLIPAGTVFKGDN"
     gene            complement(4035..5120)
                     /gene="dmlA"
                     /locus_tag="ROI21_003297"
     CDS             complement(4035..5120)
                     /gene="dmlA"
                     /locus_tag="ROI21_003297"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDX5112180.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            5223..6146
                     /gene="dmlR"
                     /locus_tag="ROI21_003298"
     CDS             5223..6146
                     /gene="dmlR"
                     /locus_tag="ROI21_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDX5112181.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGTPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            6273..6911
                     /gene="leuE"
                     /locus_tag="ROI21_003299"
     CDS             6273..6911
                     /gene="leuE"
                     /locus_tag="ROI21_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDX5112182.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            7084..7443
                     /gene="yeaR"
                     /locus_tag="ROI21_003300"
     CDS             7084..7443
                     /gene="yeaR"
                     /locus_tag="ROI21_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDX5112183.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYPDEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            7447..7629
                     /locus_tag="ROI21_003301"
     CDS             7447..7629
                     /locus_tag="ROI21_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDX5112184.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            7777..8025
                     /locus_tag="ROI21_003302"
     CDS             7777..8025
                     /locus_tag="ROI21_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDX5112185.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8084..8158)
                     /gene="yoaJ"
                     /locus_tag="ROI21_003303"
     CDS             complement(8084..8158)
                     /gene="yoaJ"
                     /locus_tag="ROI21_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDX5112186.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8161..8259)
                     /locus_tag="ROI21_003304"
     CDS             complement(8161..8259)
                     /locus_tag="ROI21_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDX5112187.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(8292..9317)
                     /gene="dgcP"
                     /locus_tag="ROI21_003305"
     CDS             complement(8292..9317)
                     /gene="dgcP"
                     /locus_tag="ROI21_003305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDX5112188.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            9500..9754
                     /gene="yoaF"
                     /locus_tag="ROI21_003306"
     CDS             9500..9754
                     /gene="yoaF"
                     /locus_tag="ROI21_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDX5112189.1"
                     /translation="MKIISFVLPCLLVMAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(9776..10123)
                     /locus_tag="ROI21_003307"
     CDS             complement(9776..10123)
                     /locus_tag="ROI21_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDX5112190.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(10178..11359)
                     /gene="nimT"
                     /locus_tag="ROI21_003308"
     CDS             complement(10178..11359)
                     /gene="nimT"
                     /locus_tag="ROI21_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDX5112191.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     FLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDTNGDWSIPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            11456..12277
                     /gene="nimR"
                     /locus_tag="ROI21_003309"
     CDS             11456..12277
                     /gene="nimR"
                     /locus_tag="ROI21_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDX5112192.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTSTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWARFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(12234..12680)
                     /locus_tag="ROI21_003310"
     CDS             complement(12234..12680)
                     /locus_tag="ROI21_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDX5112193.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            12850..12954
                     /locus_tag="ROI21_003311"
     CDS             12850..12954
                     /locus_tag="ROI21_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112194.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(12955..13458)
                     /gene="yeaK"
                     /locus_tag="ROI21_003312"
     CDS             complement(12955..13458)
                     /gene="yeaK"
                     /locus_tag="ROI21_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDX5112195.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(13501..14991)
                     /locus_tag="ROI21_003313"
     CDS             complement(13501..14991)
                     /locus_tag="ROI21_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDX5112196.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            complement(15172..16647)
                     /locus_tag="ROI21_003314"
     CDS             complement(15172..16647)
                     /locus_tag="ROI21_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDX5112197.1"
                     /translation="MIRSTRVSLGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MASMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            complement(16794..18077)
                     /locus_tag="ROI21_003315"
     CDS             complement(16794..18077)
                     /locus_tag="ROI21_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDX5112198.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(18190..20124)
                     /gene="yeaG"
                     /locus_tag="ROI21_003316"
     CDS             complement(18190..20124)
                     /gene="yeaG"
                     /locus_tag="ROI21_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDX5112199.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            20561..21307
                     /gene="mipA"
                     /locus_tag="ROI21_003317"
     CDS             20561..21307
                     /gene="mipA"
                     /locus_tag="ROI21_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDX5112200.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            21397..22251
                     /gene="yeaE"
                     /locus_tag="ROI21_003318"
     CDS             21397..22251
                     /gene="yeaE"
                     /locus_tag="ROI21_003318"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDX5112201.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYAEGGAEMVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAKGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     EKTALDMV"
     gene            complement(22302..23186)
                     /locus_tag="ROI21_003319"
     CDS             complement(22302..23186)
                     /locus_tag="ROI21_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDX5112202.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKACEIDLEAHGEFETT
                     SALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYTSETQK
                     VTKEKPAHLAQSIRVAKR"
BASE COUNT         6083 a         5579 c         5686 g         5865 t
ORIGIN      
        1 caaatccaac accaggcgtt tacccttcgc acgcgaacaa cagatcaaca tactttgctg
       61 gctggcacgc tcttcatcgc taaaatactg atcccgatgg tcagcttcac cttccagtat
      121 tgccgtttcg caggttccgc atacccctca cgacataaac attccacttt cgcggcttta
      181 ttattttcaa taacctgcaa aatagtcatc tcttccggca ccacaaactc tttaccggaa
      241 cgggcaagca ccagggtaaa tgcatcgccg gttttgtctt caatcgcaaa ttgctcaaag
      301 tgcagcgtat cggcggcgat gtccagacgc gcagcttcac ttcttaccgc ttcatttagc
      361 gcctcggggc cacaggtata aacatgtgtg ccaggttcgc tatccgccaa taatcgcgct
      421 aattccagtc gtgttccggt tgatgaatga tgcaaatgga ctttctcagc ctgcggatgc
      481 tggactagct catcacgaaa tgcgcaactt tctggatttc gcgtgcagta atgtagctgc
      541 cagtcgacgt cgctgtgttg caactctgcc atgtgcgaca ggaaaggggt gataccaatg
      601 ccgcccgcga taaacagatg ctttctggcc gagggaatta gcgcaaacag gttattaggc
      661 gttgaaatcg ttaaccgatc gcccactttt acctgctgat gtaaaaagcg ggaaccgccg
      721 cgcgagtttt cctccagccg aacggcaatc tgataacaag aggtgtcatg cggcgaactc
      781 agtagtgaat acgcattgct gtactcgtta tcaccatcgc tcatctgcac aatgacgtga
      841 cttcctccgg taaacgcagg taatggtttg ccatcggttg ccaccagcat gaagcgtttc
      901 acctgttcgg taagcggttc aacctggctc acctgtactt caaacatttg atagtctgac
      961 attgctgtac tccatcaaaa atcagcccag cggcggaaaa taccgccgcc gatgtcatta
     1021 gtccttaaac acctgcgcca gcagattatg gaagtgggcg ataccatgtt cggaaattcc
     1081 gctaccgcta ctgtcggcca tgatgcgccc ctgaccacga tagccacgcg atttcagccc
     1141 tttctgtacg ctttcaacca gacgtaaatc ttccggacgg aacacatcgc gataccactc
     1201 aatcagcgat ttttgctcgt cggttaactc ttcattggtg aagtaaatat cgtagttttg
     1261 cagggtagtt tcagaatcca ccgggaattc ataaatgacc gtcatcatcc ctttgattgg
     1321 ggtgacgttc agcatcgtgc acggccacag ccagaaaccg tggaatgccg catccgtgcc
     1381 ctcttcgaat ttgaaagact gttctgacgg tttggcgaaa ccgtattgca gcgtccagtt
     1441 accgtgcatg gtgtgccagt aacgatcaac ctgaacggag tcggagaagc ctggatgcgc
     1501 cggaccacag tgatagcact cgagatagtt atcgacaatg ttcttccagt tggcaggcgt
     1561 gagggtggta aagcgggccg ccagtttcag atcgtggact tccgggcagg cttccagcac
     1621 ttttgcgccc agacctggta actgatcttc tacgctggtg gcgttggggt ccatgttgat
     1681 gaagacgaat ccggcatatt cttccagacg caccggaacc agttgcgctt tgtcgctatc
     1741 gaaattggcg acgttttcgc agttacgcgc atgggccagg ttaccatcga gtttgaatgc
     1801 ccaggcgtga tacgggcagg taatcacatt tttcgctttc ccttcgccgt tcaataactg
     1861 atgaccacgg tgcgggcaaa cgttatagaa cgcgcgcaaa accttatcac gaccacgtac
     1921 cagcacgatg ctttcgccaa tgatctcacg cgtcacataa tcattggcat tcgccagttc
     1981 gctgctgtga gcgacgcaaa tccagctttt ggcgaagacg ttctcttttt cgtgttcaaa
     2041 ggcgttctga tcggtataaa aacgggcagg aatggtccac gcctcttgcg ggttagcgca
     2101 aaaattttcg ggtagtacaa agtcagggct cagattgctc atagggatac ctttatcgtt
     2161 aggatgatgg cgctcagttg tcgcctttga atacggttcc tgccgggatc agcggatttt
     2221 tttccgatgc ttctaccggt gtctgcggga gataatcttc aacttgatgg gccgggatat
     2281 cggcgtaatc ctgcttcagc cagcgaataa agccaccgac tttcaccagt aaaataacga
     2341 ggaagggcag ggctgtaagt accacagtgg ttttcatggt ttccagcgac gcgccagtaa
     2401 ataagataga cagtggaatc agggtgatca ccacgcacca gaacaggcga agagcacggt
     2461 cagggtcgtc accttcctta agatttcgcg tactggtcgc cgccatggtg taggccaccg
     2521 catccatatg cgaggcaagg aaaataatca tcacgcccag gtatgcggcg aggaacaatt
     2581 taccggctgg caacgacatc agaacttgct gcacagctgt ctcgccgccc agtgtttcca
     2641 gcacctgtgg gacgttgatt acgccattga taaactggtg aatggcatag ctttccatta
     2701 cgccgaagaa gaaccagcaa ccgacggtgc tgccgaggat cagtccccag ataacttctt
     2761 taatcttacg accgcgtgaa acgcgggtga caaacattgc tacgccaggg gtgtatgaga
     2821 tccaccacag ccagtagaaa acggtccagt tgcgggtaaa tgaaccatcg ccaagcggat
     2881 cggtgaataa gctcatttgc aggaagtttt gcgtggtcag gccgatggca ttgatgatgc
     2941 tgttggtaat aaattcggtt ggaccgacaa tcagcaccag taatggcagc aggaacgcgc
     3001 cccagccaac cattttgctc agacgttgca aaccgttgtt gataccaatc cacgagctta
     3061 ggcaaaaaat gccgccggaa agcaggatca caaatgcctg cacggtgaag ttatcgggta
     3121 aaccggtcag cgcggaaagc ccacgagtaa aggttgctgc ggtaacaaca agggaaatgg
     3181 tcagcgcacc gacagtggcg atcaggaaca tcaaatcgac cagttttccc cacgggcctt
     3241 gcgggcgaac gccggtaatc gcggcaataa tgccggaaag gctcagacct ttgtttttcc
     3301 gtacatgaaa gtgataagcc atgattaatg aggccagcgt ataagttgcc caggcgctga
     3361 tgccccagtg gaagaaggag taagggacgc taaattcgag tgcctgttgt gaacgcggtg
     3421 cgatatttaa tccaggtgtt tgataataat aggcccattc agcaaccccc caataaagcg
     3481 tagaagaacc taaaccggca caaataaaca taaacagcca ggagagcgtg ctgtattccg
     3541 gttttccttc tccaagacga atattgccgt atttactggt ggctaaataa atcaccagtc
     3601 ccattgccag caaaaccaat acctgtacgg cggaacctaa cgtacgcgta acaaaagtgt
     3661 atattgcgtt ggccgcgtcg gccgattgtt gtgggaaaag gcttaatgca gctgcgatga
     3721 aaagaatggc caccaggctg atgcttatca gcggcacatc ttttttctta acattgctca
     3781 tcatgagtca tctcctgggc ataaaagccc gtctctggct tttgccagtt gtaattattt
     3841 gcagggtaat gagttgcatg ttgtgtttaa caaattgatt atttgcatgt tacataaatt
     3901 aaacaattaa gaatacggaa agccataaaa cacgaaacgg aaacaataaa aggtgaaatg
     3961 acgcaggtta ttaactgcaa agaacgaata aaaaaaccgc gtcagtgacg cggttcaaat
     4021 aaactggttt gaccttaacg caaaataatt ttgcaaatcg cgtcggcaac ctgtggcgtg
     4081 gtggcactgc ctttcatatc cggtgttttc ggcccgtgag caatcacttc ttcaatcgct
     4141 gccagaatac cgttatgcgc ttgctggaaa cgctcatcgc cattgccgag aaaatcgagc
     4201 atcattgcac cggcccaaat tgtggcgata gggttagcaa tgtttttccc gtagatatcc
     4261 ggcgcggaac cgtggactgg ttcgaagagc gacgggaaag tgcgttccgg attcaggttg
     4321 gcggatgggg caatgccaat ggtgccggtg caggccgggc caagatcgga aaggatatcg
     4381 ccaaacaaat tggacgccac taccacatcg aagcgttccg gctgcatcac aaaacgtgcg
     4441 cagagaatgt caatatgctg cttgtcccag cggatctccg ggtaattctc agccattgct
     4501 tccactcgct catcccagta cggcatgctg atggctaaac cgttcgattt agtggcagaa
     4561 gttagcgtct tacgtgggcg gctttgcgca agttcgaagg cataacgcaa aatgcgatcg
     4621 acaccacgac gcgtaaatac cgattcctga atgacgactt catgctctgt accttcattc
     4681 actctgccgc cgagcgagga atattcgcct tcggtgtttt ccctgaccac gtaaaaatcg
     4741 atgtcgccag gctgttttcc cgccagcggg cagggaacgc caggaaagag acgaaccggg
     4801 cgcaggttga cgtactggtc gaattcacga cgaaatttca gcagcgaacc ccacaacgaa
     4861 atatggtccg gaacggtgtc cggccagccg acggcaccaa aatagatggc gtcgaaacgg
     4921 ctaagttgct catgccagtc gtccggcatc attttaccgt gatggctgta atactcgcag
     4981 ctcgcccact ccatttgctc aaaactcaag gcgaagcccc agcgctcagc ggcagcctgt
     5041 aacacgcgaa tcccttcagg aaggacttct ttgccaatcc cgtctcccgg gatcgcagca
     5101 atacgcatcg ttttcatcat tgggttctca cttcctgacc atcaaaattg acaacatcac
     5161 catcctataa ttgactgaaa aggaattaat ccctgtaact ggtgaaacat aaaacacgaa
     5221 tcatgaataa tttaccgctg ctgaacgatt tgcgcgtctt tatgctggtg gctcgccggg
     5281 ctggttttgc cgccgtggcg gaagaactgg gcgtttctcc ggcgttcgtc agcaagcgca
     5341 tcgccttgct ggagcaaacg ctaaacgtgg tgttgttaca ccgcaccacc cgccgcgtca
     5401 ccattaccga agaaggagag cggatttatg aatgggcgca gcggattttg caggatgtcg
     5461 gtcagatgat ggacgaactt tctgatgtgc gtcaggtgcc gcaggggatg ctgcgtatta
     5521 tcagcagctt tggttttgga cgacaggtag tggctccggc gttatcagcg ctggcgaaag
     5581 cgtatccgca actggaatta cgttttgatg tggaagaccg gttggtcgat ttggtcaatg
     5641 aaggtgtcga tctcgatatt cgcattggcg atgacatcgc ccctaattta atcgcccgca
     5701 aactggcgac caattaccgc attctctgcg cttcgccgga atttattgcg caacatggca
     5761 cgccgaaaca tttaaccgat ctttctgcat tgccatgtct ggtcattaaa gagcgcgatc
     5821 atccttttgg tgtctggcaa ttacgcaata aagaaggcca ccatgcgatt aaagtcaccg
     5881 gcccactgtc gtctaatcat ggagaaattg tccatcagtg gtgtctcgat gggcagggga
     5941 ttgctctgcg ctcctggtgg gatgttagcg aaaacattgc cagtggtcat ttagtacaag
     6001 tgctaccgga atattaccag ccagcgaacg tctgggccgt ttatgtttca aggctggcga
     6061 cgtcagcgaa agtgcggata acggtagaat ttttacgcca gtattttgcc gagcactacc
     6121 cgaatttttc actggagcat gcctgattaa tgattcaatt atcgggttga tatcgggtta
     6181 aaacctgatt ttctcctttc taagccgcta cagattggtt agcatattca cctttaatcg
     6241 cgcatgatcg aaagataatt aaagaggtta atgtgttcgc tgaatacggg gttctgaatt
     6301 actggaccta tctggttggg gccattttta ttgtgttggt gccagggcca aataccctgt
     6361 ttgtgctcaa aaatagcgtc agtagcggta tgaaaggcgg ttatcttgct gcctgtggtg
     6421 tatttattgg cgatgcggta ttgatgtttc tggcatgggc tggagttgcg acattaatta
     6481 agaccacccc gatattattc aacattgtac gttatcttgg tgcattttat ttgctctatc
     6541 tggggagtaa aattctctac gcgaccctga agggtaaaaa taacgaggcc aaatccgatg
     6601 agccccaata cggtgccatt tttaaacgcg cgttaatttt gagcctgact aatccgaaag
     6661 ccattttgtt ctatgtgtcg tttttcgtac agtttatcga tgttaatgcc ccacatacgg
     6721 gaatttcatt ctttattctg gcgacgacgc tggaactggt gagtttctgc tatttgagct
     6781 tcctgattat ttctggtgct tttgtcacac agtacatacg taccaaaaag aaactggcta
     6841 aagtgggcaa ctcactgatt ggtttgatgt ttgtgggttt cgccgcccga ctggcgacgc
     6901 tccagtcctg atgcattcag cccgcgttgt cgcgggcttc ccacctgtaa tcctccctga
     6961 ttcttctctg atacggtgct aaatagtaac caataaatgg tatttaaaat gcaaattatc
     7021 aggcgtaccc tgaaacggct gaaataaacc gttttcagcg cattcaccga aggagggaaa
     7081 aggatgctgc aaatcccaca gaattatatt catacgcgct caacgccttt ctggaataaa
     7141 caaactgcgc ctgccggaat attcgaacgt catcttgata aaggaacgcg cccgggggtt
     7201 tacccacgcc tttccgttat gcatggggcg gtcaaatatc tcggctaccc tgatgaacac
     7261 agtgcagagc ctgatcaggt gatccttatc gaagcggggc agtttgcggt gttccctcca
     7321 gaaaagtggc acaacattga agccatgact gacgatactt atttcaacat tgacttcttc
     7381 gttgctcctg aagtcctgat ggaaggcgcg caacaacgga aagtcattca taacgggaaa
     7441 tgaatcatgg gcaaagcaac gtatacagtg accgtcacca ataacagtaa tggcgtttct
     7501 gtcgattatg aaacagagac gccgatgact ttactggtgc cagaagtggc ggctgaagtg
     7561 ataaaagatc tggtgaatac cgtgcgttct tatgacacgg aaaacgaaca tgatgtttgt
     7621 ggttggtaat tattactgat cgttgatggc gcaatttctg cgccatcgca aaaaaatcct
     7681 ccgtgatgct cgtcacaaat cttgccctcc cgatgttgtg ctatgctttt tatcagcgac
     7741 taacccgcta atgcaaaaca gataagtgag gaaacaatgg gcattctgtc atggattatt
     7801 tttgggctta ttgccggtat tctggcgaag tggatcatgc caggtaaaga tggtggtgga
     7861 ttctttatga ctatcctgct ggggatagtc ggtgccgtag tcggcggatg gatcagcacg
     7921 ctgtttggct ttggtaaagt cgatggcttc aattttggca gcttcgtggt tgccgttatt
     7981 ggcgcgattg tcgtgctgtt tatctacaga aagattaaaa gttaacgcgt aaattgcaca
     8041 aaggctgcac acaggcagcc tttgctattt tctagatgtg acgttaccac catcccagct
     8101 cagtgccgag tacgacaata atcgccacac ccatcataat aatcgttgtt tttttcatct
     8161 ttatgctccc ggggcagcat agccgccaat aaaaaaccat gctaaaaata cgaccgctgt
     8221 aataaagatt acaaccggaa aaataatacc gattcgcatg ttatcaccaa tcaaaagaat
     8281 gtgaatacgc ctcaggaatg tagcgctgga tgcgcgacaa aaggtgtttt ctgtttgtgt
     8341 tttttctcct gatacatcgc gatatcggca gcatgcaggg cactgtctgc atcggtcgtt
     8401 tcaggatcga cttctactat gccaagactg gcaccgggat aatacaaatc aacatcacct
     8461 aagtgatatt ctccgcgtat ttgctgctga atacgttctc gcagagacga aatatccgca
     8521 ttctcgttgt tcagtgaaac aaccaaaaac tcatcaccgc cgagacggcc aataacttcg
     8581 ccattttgct ggagcgtatt aaggcgctcg ccaacctgaa tgaggaacaa atcaccacta
     8641 ttatgaccaa aacgatcgtt gattaatttg aagttatcca gatcgataaa cgcgatcatt
     8701 atcttatggt taagatgccg ggcgagagaa aacagcgtcg tcaggttttc aaaaatcgcc
     8761 cgccgattcg gtagcccggt taacgagtcg gtatacgatt gcgcaatcag cgcagcattg
     8821 gcttcgcgca gctgttcaac cagtgcctct ttttgaatat attgtgcaat cagtccggcg
     8881 aataactgta aaacctgttc cgcgcgttca ctccactggc gcttctcact actggcggcg
     8941 caaagcgtgc catagaatga tccatccggt aagtgaattg gcgtgctcag aaatgtggtg
     9001 atgccaagat tgcgtgccgc aatacagtcg ccccaacggt cagggacttc atcgctgaaa
     9061 aagcagtttt catcaatggc gcgtttgcat aacgagtaat cccacgagac ggtaaaattc
     9121 tccgggatgt gcattttctg gctgttacgg gcaaacataa tatgctgcag gcgcgcttcg
     9181 acatccactt tggtcaggta ggttgattcc atatcagtga ccatttccag catctccaga
     9241 agctgtcgga ccagactttc cagtgactgt tctttggcaa gggattgcga gacgcgggcg
     9301 ataatctgat ctgacacgaa acaactccag gatacggcgg gtaaatgctg attatttaat
     9361 ctttgcaggc atgataaaac atcatcaaca aagatgaaac aaggcgtaat atgatttgag
     9421 tgaatgtaat aaaaatagtg ctgcaaaaca aaacgatacc gcgatactct aagtctggat
     9481 aaatatgtga gatcaataaa tgaaaattat cagttttgtt ctgccttgct tgctggtcat
     9541 ggctggttgt tcaacccctt ctcagccaga agcacccaaa ccgccgcaga ttggtatggc
     9601 aaatccggcg tcggtctatt gccagcagaa gggcgggacg ctcattccag tgcagacagc
     9661 gcaaggggtc agcaacaatt gcaaattacc gggcggtgaa acgattgatg aatgggcatt
     9721 gtggcgacgg gaccatccgg ctggtgaaaa ataaccgccg gatgctgtac taaaatcaca
     9781 agctacgtag ccagtcggcc agcaccagcg catggttctg cgtggtgttt ttggctgagt
     9841 agagcagggt cagcggctgt tttttggcga tgtccgccag ccgctttcct tcttgctcgt
     9901 gttgcgccag ttctgcaaga tattgctcgc gaaaggttgc aaaatcgacg acttcgccgt
     9961 gaaaggcttt gcgcagttcc gttgacggcg tgatttcttt atcccactca tcaagggcta
    10021 aatcggtttt tttgatacca cgcggccaga ggcggtcgac cagtacgcga taaccatcgc
    10081 tctgttctgc cggatcataa acgcgtttgc actgaatatt catcgtgttt gttctccctt
    10141 ccacgcatga ttggtcacgt cactatagca ccggatatta gcgaatttct ttgtctctcc
    10201 cggcgcaaag tccgaaaatc gccatcagta gtgaaagtat ggcaacaccc atcaatggta
    10261 tggaccagtc accgttagta tcgtgtattt tacccatcag cggcggccca caggctgcca
    10321 gcaaataccc gacggattgt gccatccccg agagtgccgc cgcctgatgc gcagaacttg
    10381 cccgaagacc aatgaacgtc aatcccagta tcattgttgc gccggaacca aagccgaaaa
    10441 gcagagtcca ggtgatcgcg tgcgctggca taaagcagag cccaaccgcg cccactgcgc
    10501 acatcaaggc aacgaacgct gcaataccac gctgatcttt cacatgatgt aagaaaagtg
    10561 ggatcagcaa accgggagct gctgtggcta gttgcagcaa accatgcagt gaacccgcct
    10621 gtgcttcgct atagccgtga ctgatgagga tcgccggaag ccagccaata atcacgtaat
    10681 agaccagtga gttgatccca agaaacaatg ttacctgcca ggcaagcgga gaacgccaga
    10741 taccccgagt atgtaaggcg cgtgaggtac tcaaatttgc atgttgttga cttcgccact
    10801 gtggcagcca taaaaaaaga gccagcagag gaaaacacat cagcatgagc aacgcgcctt
    10861 gccagccaaa accgttcaaa gccagcggca cgaccatagc cgatcccagt gccgctgcag
    10921 ctcccattgt cagggaatat gcgccggtaa gtctggcgac ggaatgaggg aaatcgcgtt
    10981 taattaatcc tggcagtaag acattgccta atgcaatccc accgccaatg accgctgtac
    11041 cgccaaataa gaagtaaggc gaagggagag agcgaattgc gatgccagca cagatcaaaa
    11101 gtaacgcggc aaacaggcta cgttccatac caaaacgtcg cgccaccggg gctgccagag
    11161 gtgagattag cgcaaaggct aataatggca gggtggtcaa taagccggtt tgcgccgtcg
    11221 tcagcgagta agcggaacga atcgtatcca gtaacggtgc tgcgccggta aaggtgacgc
    11281 gtaatgttgt ggcaatcatt agaatgccag cgataaggac aatcctgttt ttgccgctta
    11341 atgaagttga acaggtcata tgcttctcat tgttacaaac gaggctgtac cttaccgttt
    11401 ttattgttat gttgaatcaa gctaaaatga caaattatcg ctaaattcgg acaaaatgat
    11461 gcacaggttg aatctcaatg gctatgaacc cgaccgacac catgaagccg cggtcgcatt
    11521 ttgtattcac gccggaacgg atgagttaac cagtcctgta catcagcacc gcaaaggaca
    11581 gctgatactg gcgctacatg gtgcaattac ctgtacggtg gaaaatgctt tgtggatggt
    11641 gccaccgcaa tatgcggttt ggataccggg cggggtggaa catagcaatc aagtgacagc
    11701 taacgctgaa ctctgttttc tgtttatcga acccagcgca gtgacaatgc cgacaacctg
    11761 ttgcacattg aaaatatcgc ccttatgccg ggaattaatt ttaaccctgg caaacagaac
    11821 gtcgactcag cgtgctgagc cgatgacccg cagattaatt caggtcctgt ttgatgaact
    11881 ccctcagcag ccacaacaac agctgcacct acccgtttct tctcatccta aaattcgcgc
    11941 gatggtggag atgatggcga aagagcccgt cgagtggggg tcattggggc aatgggctcg
    12001 cttttttgcg atgagtgaac gcaatcttgc ccgactgata gtgaaagaga cggggttgag
    12061 ttttcgccag tggcgtcagc agttacagct gattatggcg cttcagggat tagtgaaagg
    12121 cgatactgta cagaaagttg ctcatacgct ggggtatgat tcaacaacgg cctttattac
    12181 tatgtttaag aaaggcctgg ggcaaacgcc aggccgatat attgcgggat taactactgt
    12241 ttccccacaa tcagcgaaac cagacccgcg gcaataagcg ggccaaccgg tacgccgcga
    12301 aacagcgcca cgcccaaaac agtgcccacc agcagcccgg cgaccagttg cggctggctg
    12361 cccattaacg tcacgccgcg cccacctagc caggagacaa taacgcctac tgcaattgcc
    12421 accagcgatt tccagttcag aaacgagtga atcagggttg agggcggtag cgtcccgctg
    12481 gcgatggggg ccataacgcc aatcgtcaga atgattatcc cgatacttag cccctgtttt
    12541 tcaatccacg ggaaaaaggt gcttaacggt gtgacgcgga caatgattaa caccagaatt
    12601 gaaacggcga cagtggtgtt atgactgata aagcccagcg cagctaaccc gagcaggatc
    12661 agcaaagtga catcaaacat cgtggtttcc ttgccaaaaa ataataagcc tgcttacttt
    12721 acgcttgctg caggaaggaa gtagaaaatc tttacgtttt ttgcataaaa aatcaggcga
    12781 actgtcatca aactgcaatt tgtgcacgct aacaataacg tgaaaacagc tatctcccgg
    12841 gggcatacta tgaacgatca aatgttcgtc gagacactga ttatcacgtc atcgtttttt
    12901 gccattgctg ttgtgctggt tttgtccgtt cttctaatcg aacggacagg ctagttacgc
    12961 agtgcggcgg aaattgacca gttctggttg cgcaatgcgc aaataatccg cggttttcaa
    13021 aataacggat ttatccagca tgcctgcatt gaaggcgatt tcatcaaatc gttcgaagag
    13081 taggggatcg gcaaccagtt tgagttttgg atggaagctg aaagggggga tcgcgccgaa
    13141 gacacagcct gtgagttcat cgacttccgc cgggctggcc aacgaagcgc gtaatccgcc
    13201 tatatggctg gcgagttggc tcagatcggc ctgttgatcg gccgcgagga tcgccagaac
    13261 atgttgattt acaccattgc ctttgacttt acataccagt gccttagccc cctggcctaa
    13321 agcggtgcca cgaatttctg acaccgcttc acatttacct accgcttcat gggtaacgac
    13381 gcggaagtca gcaccttctt gtgataataa agcgattaac cgctgatgcg tcacgcttcc
    13441 tttagccatt tcagtcattt catgctcctg agattacaag caaacaacca cagaaggtta
    13501 tcatgaacgg ctgtttttgt tctgcttatt gacatataaa cgctcatcgg aagctttata
    13561 cgcatcatgt aacgtatcgt tttctttcat cgcgtaaata ccggaagaga agccgatctc
    13621 tttctgcggc gcgatatgtt gcagacgttt ttcgatacgt tcaggcagtt gtgctgcaat
    13681 ttgcggcgtc gaatcgacta gaatgatgca gaattcatcg ccaccgagtc ggatggcata
    13741 atcactttta cgaatcgact gtttaatcgc ctgagctaat aacgtaatcg ctaaatcccc
    13801 ctcctgatga ccgagggtgt catttatttg ctttaacttg tccatgtcaa ttgcaataaa
    13861 catcaccgaa gaaccggatt gcaccagttt ttgcaaccgc tgctccagtt caggggttaa
    13921 aattttgcga ttatacagtc ctgtcatcgc atcactaata ttttctcgcg acacattttg
    13981 gtataaacgg aagtgcatcc gcaccatatt cagcaaaatt aacgccgtca aaatccagaa
    14041 cagaacgctt ttccacgaag acgtgataaa gtaaagaata tcaatggata acgagacacg
    14101 aatgccgccc ggtaagtcat ggacgtaact gacatactgg aacagattat cttcgctctg
    14161 gttgatgata atgtcgcgcc ccgaatcggt atcggttagc gtgacattga gaaaacgcca
    14221 gaggagaggg cggtcatgag tgtaaaagat attccgcaga ttgtttttat taatatccag
    14281 cagcacaatc ccttttagct gccctgcgac atataccggc gtcagcaaac tcatcacgtt
    14341 ctgtttagta cgctgatcct ggtaaatgct cgataaaacg gtacgcccgg taaatacctt
    14401 ttcaatatct ttgcgatcga tgccaatact tcctgactgc aggaaagacc agttattcat
    14461 cgcaaagtac tcaacattaa ccggctcata aaaatagaca tagcggtcat tgagatcgag
    14521 ataatagtga aatttctctt cggccatccc aagacctgaa ccgtaatcat cctggtggcg
    14581 gttgccttcc actgcctgat caaaagcggg taatagtgca gcagcttcca caattgtgtc
    14641 gcagtcagtg gatgcggttt gtagtgtgcc gcgtaagccc gggtatttat gggcagtcag
    14701 gtttaagcca tgggtattat tggcagactc aaaagcctcg cagatggcgc gccgctgttc
    14761 tggcgatact tccgactgct cagcagcgag agcgcgcatc acatgcgcgg caatactctg
    14821 attctgatac ttatcataaa gaaaggagga gtctgctctc tgcacgatat agcgcagata
    14881 ggcactcatt gctctgtcgc tggcgacaag ttcaaaaata aggaaggaag atgtcagcac
    14941 aatgacactt gcggcgataa aatgccggag cattctatgg tgcaatttca tgataaatgg
    15001 cactccttcg gttagtgaaa cgagaaaacc agataaagtt cgattgcggc ctggcacagg
    15061 ggcgctaatt atactcatct ctgcgccgct ggggagggga aatttagggg taatagtaat
    15121 aaattgtatg caaaagccac gcaaaaagca tggctctgaa tatttttgca cttaaatatg
    15181 atgaataatc actttgttgc ccccctcgct tttcgcttcg cgtaaggctt tatctgcttc
    15241 cgtcatgaca agcgagatat tacgtgtgtc acccgttgaa aaaaccgtgc caatactgat
    15301 ggtcatttgc tcaggaacat catatctgtt actaaagccg gtcaggagtt cgacattttt
    15361 acgcatacgt tccgcaatga tttttgcctg ggcactattg agttcggtaa atagcaagcc
    15421 aaacacctca ccctccagtc gcgctaaaat atcatcgggg cggatgcttt gctggataat
    15481 atcgacaatt gaaaccagca ctttatcacc cacacgatgt ccccattggg cattgatgcg
    15541 tttgaactga ttaatatcaa ccagcatgac acagaagtcc ttaacaacag gtcgcgacaa
    15601 taatgactct acgctgttga aaaagtagcg ccgattataa atattggtca gtacatcatg
    15661 aactgccagt ttgctggaga gttgcagttc ctgaaaaatg ttataaatca gaaaagacac
    15721 cacaaacagt ttactggaaa cttcaatccc gcgactgata taccagatgg tgtaattata
    15781 ctcatccaga gtcagtagaa gtaagttgca gcatagcgat gccagacata ataatgctat
    15841 taacggccat agttcgctgg ctaatcgtgt acgcatgata ataaagaaca gtaaaaatgc
    15901 ccacagacaa accaatattt ttgtatagtt gattccccag gtcgcagtat ggttgtccgg
    15961 acagtaatcg acgacataca aagaatagtc agcgttataa ctgctcagat tgtgtgccag
    16021 aaggggaaaa actaaaaaag gaatcagcgc cagtaacaga atgccagttt ttttcttatt
    16081 gttgtcaagg atgttgtctt tcccataaca aaagagcgcc agcgaggtta aacaaatgaa
    16141 actgagttgg cggaaaatat agaaaataga aacatcattc gactttgtct ggatcagtgt
    16201 actgccgttg attggcttat ggataattac aatggtttca actaaataga taaggccact
    16261 taaaaatgca aggcttaaaa tgagtgttgg taaaatttgt ttgttagtga aatacttcat
    16321 taacatgaaa agaaaaatga cggcgttaag gaaaaacaaa gccacaacaa ccatcaggta
    16381 attcatctgt gggatgagtt ttacattcgt gttttcgtta tataaggtag taatgcaaaa
    16441 taaaattaac gtcagcaata agctgaaaat aaaaaagcct tgagctttta atttttgtgt
    16501 acccatagaa gccataaaaa ggagagcaat gtgagtgctg gattttattg atggcagaga
    16561 tatatagttt tgaccaggat caattttcgt taacgataaa aaatatttaa agaataaccc
    16621 cagggaaaca cgtgttgacc gaatcatttt tccgaatgat tgttttctgg aactaaattg
    16681 tgtaaaggtt atgctgtcat atttaaagcg attgtaagct aatgtatgta ataaattaga
    16741 taatttacag tgagctaaaa atcagccagg cgataatgac ccggctgata gttttagtct
    16801 ttagctgttg cattttgttt atgaaacagt tcacggaaca ccggataaat atcatcctgg
    16861 tcgcggatat gctgcatcgc aaagttgtcg aaagtagatt gcagatgctc atattctcgc
    16921 catagtgtct gatgtgcacg acgggtaatt tcgatatagc tgtaataacg aacgacgggt
    16981 aataattttt tcgccaggat ttcatggcaa agcggagagt catcggccca gttatcgccg
    17041 tccgatgctt gtgccgcgta aatattccac tgtgccgggt tataacgctc tttcactacc
    17101 tcatccatca gtttcagggc gctggaaaca atggtgccgc cagtttcctg cgagtagaaa
    17161 aactcatgtt catcgacttc tttcgcctgg gtatgatggc ggatgtatac gacttccacg
    17221 ttcttatacg ttctgctaag gaacagatac agcagaatat aaaaacgctt agccatatct
    17281 ttagtggact gatccattga accggaaacg tccatcaggc aaaacatcac tgcctggctg
    17341 gagggatcgg gccgcttctc gtagttcttg taacgtaaat cgaaggtgtc aataaaaggg
    17401 acgcgttcaa ttttggcacg taattctgca atttctttgc gcagacgttc ctcttccagc
    17461 agctgcgcag gttcactgtt gctgatgatg gccaaattct cttccagtgc atgaagttcc
    17521 cgccgcttgc ctgccgtcat ggctgtgcgt cgcgccagtg agttctgcaa ggaacgcaca
    17581 acgctgatat tggccggaac gccgttagcg gtataacccg cccgatgcgt tttatattcg
    17641 gtcagctggc gttgttggtt ttgtttcaga ttcggtaagg ccagatcttc aaagagcaga
    17701 tcaagatact catctttcga aatctgaaag acaaattcat cctgaccttc accatcctgg
    17761 ctggcctggc cctgaccact gccggaacct ccgccgccac cctggggacg ttcaattcgg
    17821 tcattctgga cgaaatggtc attgcccgga tgcacgcggt ggcgcagacc gccacgcccc
    17881 tgatgaaaca tcggttcgct aatatcttcc gtgggaatgg atacagactc gccgctgtcg
    17941 acgtcagtca ccgaacgctt attaatggcc tcggagatcg actgtttaat ttgcgcttta
    18001 taacggcgta aaaaacgctg gcgattcacc atgcttttgt ttttgccgtt cagacgccgg
    18061 tcaataaacc aggtcatatg ccccccgtac tacatttgcc aactgtatgc tacgcctgtg
    18121 ttgccgcttc tctggcccac ggttcgctgt cgttgtaggc ccggtagcat gtgtaccggg
    18181 ccagggttgt taagacgatt tacgtacgcg caaataccat tcgcacagta aacgcacctg
    18241 tttacgggtg tagccttttt ccatcatacg gtcgacaaaa tcgtcgtgtt tcttttgctc
    18301 gtcggttgac gttttggcgt taaacgaaat aaccggcaac agctcctcgg tattggagaa
    18361 cattttcttc tcgataaccg tgcgcagttt ttcatagctg gtccagttcg gattgcgtcc
    18421 gctgttattc gctctggcgc gcagtacgaa gttaacaatc tcgttgcgga aatcttttgg
    18481 attactgatc cccgccggtt tctcgatttt ctccagctcg gcgttaagag actcgcgatc
    18541 aaacaactgc ccggtatccg gatcgcgata ctcctgatcc tgaatccaga aatccgcgta
    18601 ggtaacataa cggtcgaaaa tgttttgccc atattcggaa taggattcaa ggtaggccgt
    18661 ctggatctct ttgccgataa actcggcata tttcgggatc aagtaacctt tcaggaactc
    18721 cagatagcgt tccgcctgtt cttgcgggaa ctgctcgcgc tcgatctgct gttccaggac
    18781 gtagaacaga tggaccgggt ttgccgccac ttctacatga tcgaagttga acacgcggga
    18841 gaggatctta aacgcaaaac gcgtcgacag accgttcatc ccttcatcga caccggcgta
    18901 gtcacgatat tcctgatacg acttggcttt gggatctgtg tctttcagac tttcgccatc
    18961 ataaacccgc atctttgaat aaatgctgga gttttctggc tctttaaggc gcgaaagaat
    19021 ggaaaaacgt gacagcgttt ccagcgtgcc aggggcgcag ggggcgtgag tcaattcact
    19081 gtgattaagc aatttctcgt agattttgat ctcttcggaa atgcgcaagc aatacggcac
    19141 cttcacgatg taaacacgat cgaggaaggc ttcgttgttt ttgttattac ggaaagtgac
    19201 ccattcggat tcgttcgagt gggcgagaat aatcccgttg aacggcaggg cggagatacc
    19261 ttccgtcccg ttgtagttac cttcctgagt ggcggttagc aagggatgca gcactttaat
    19321 cggtgcttta aacatctcaa cgaattccat gatcccctgg ttggcgcggc acagtgcacc
    19381 ggaatagccg taggcgtccg ggtcattctg cgcgtagtgt tcgagtttac gaatatcgac
    19441 tttcccaacc agcgcggaga tgtcctggtt gttctcatca ccgggttccg ttttggcgat
    19501 agcaatttgt tgcagaattg acggccagac cttcactacc cggaacttag tgatatcgcc
    19561 accaaattca tgcaggcgtt ttgccgccca cggcgacatg atggtgccga gataacggcg
    19621 aggaatgcca tactcttttt ccagaatctg cgcatcttcc tgcggattga aaagacagaa
    19681 cggatgatcg ttgaccgggc tacgctcacc gttggcgctc aatacataaa tcggtacgag
    19741 ctgcattaat gatttcagtc gctcagcaag cgatgattta cccccaccca caggccccag
    19801 cagatacagg atttgtttct tctcttccag cccctgagcc gcgtgtttca gataagagac
    19861 aatctgttca atcgcgtctt ccatgccgta aaactcttca aacgccggat aacgtgcaat
    19921 gacccggtta gaaaagagtc gagaaagtct gggttcctgg gctgtatcga ccatgacagg
    19981 ctcaccgata gccatcaata gccgctcagc cgcgttggca taagcactgc gatcttgccg
    20041 acaagtggta agaaactcct gcagtgtgaa ctcttcgtcc ttggcagctt catatcgctg
    20101 gcgatagtga tcgaatatat tcatggtatg ccgtcctttc gttttttagc acaggttaag
    20161 agccgttcat atgaatagta gaggctcccg gaagaggtaa actgaacgac gtctcttgct
    20221 aaaacacagc aactctcatg ccattttatc gacgatgaga acaacgtgcc cggtgataca
    20281 ccttcaaaat taagcgtaga tggcaaatgg aaaacttgcc tgcacggctt tcttaatttc
    20341 aatggcatat caacaactca tccataaatt ttgcataatt aatgtaaaga ccagggtcgc
    20401 cagtaacgct aaattcattt ggctgtaagc gcggtgtcat ccgcgtcagg aaaattaaac
    20461 agttacttaa aaaaaatgaa aacgtaaaaa ggttgggttt cgatgtattg acgggtaaac
    20521 tttgtcgccc gctaaacatt tgtttaagga atgattaatt gtgaccaaac tcaaacttct
    20581 ggcacttgga gtgcttatcg caacgtctgc aggcgtagcg cacgctgaag gtaaattttc
    20641 cctgggcgca ggcgtaggtg tcgttgaaca cccatataaa gattacgata ccgatgttta
    20701 cccagtaccg gtaatcaact atgaaggcga taacttctgg ttccgtggct taggtggtgg
    20761 ttactacctg tggaatgacg caacggacaa actttcaatt accgcttatt ggtcgccgct
    20821 ttacttcaaa gcgaaagaca gtggcgatca ccaaatgcgt cacctggatg accgtaagag
    20881 caccatgatg gctggtctgt cttatgctca ctttacacag tacggttacc tgcgtaccac
    20941 cctggctggc gataccctgg ataacagcaa cggcatcgtc tgggatatgg cctggttgta
    21001 tcgttacacc aacggtggac tgaccgtgac tccgggtatt ggtgtgcagt ggaacagcga
    21061 aaaccagaac gaatactatt atggcgtatc gcgcaaagag tccgctcgca gcggtctgcg
    21121 tggctataac ccgaacgaca gctggagccc ttacctggag ctgagcgcca gctacaattt
    21181 cctcggcgac tggagtgttt acggtaccgc gcgctacacc cgtctgtctg atgaagttac
    21241 tgacagccca atggtggata aatcctggac tggcctgatt tctaccggga tcacctacaa
    21301 attctgataa tgcatctttc cagggcattt tgtgcataaa aacagggcgt catgccctgt
    21361 tttgcattac ggtggtgcaa tacctgggag gaaacaatgc aacaaaaaat gattcaattt
    21421 agtggcgatg tctcactgcc agccataggg cagggaacat ggtatatggg cgaagatgcc
    21481 agtcagcgta aaacagaagt agctgcacta cgcgcgggca ttgagctcgg tttaaccctc
    21541 attgataccg ccgaaatgta tgccgagggc ggtgccgaaa tggtggttgg ggaagcatta
    21601 accggtctgc gagagaaggt ctttctcgtc tctaaagtct atccgtggaa tgctggcggg
    21661 caaaaagcga taaatgcatg cgaagccagt ttacgccgtc tcaatactga ttatctcgat
    21721 ctctacttat tacactggtc tggcagtttc gctctcgaag agactgtcgc agcgatggaa
    21781 aaattgatcg ccaagggcaa aatccgccgc tggggcgttt ctaaccttga ttatgccgat
    21841 atgcaggaac tctggcagct gccggggggg aatcagtgtg ccactaatca ggtgctttac
    21901 catctcggtt cacgagggat tgagtacgat ctacttccct ggtgccagaa acagcagatg
    21961 ccggtgatgg cttacagtcc gttagcccag gccgggcggt tgcgcaatgg gctgttaaaa
    22021 aacgcggtag tcaacgaaat tgcacatgct cacaatatca gcgcggcaca agtattgttg
    22081 gcgtgggtga tcagtcatca gggtgtgatg gcgattccaa aagcggccac gattgcccat
    22141 gtccaacaaa atgcggctgt gcttgaggtc gaactttcat cagcggaatt agctatgctg
    22201 gataaggcat atccggcacc aaaagaaaaa actgcgctgg atatggtgtg atgttgatac
    22261 gtgccggatg catcgcaccc ggcacacaac attaacgcaa attaacgttt cgcaacgcga
    22321 atggattgcg ccagatgtgc aggtttttct ttggtcactt tttgcgtttc tgaagtgtaa
    22381 gccgtttcta cacaaacaaa ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact
    22441 gaaagcgccg gtcccggatt ccagcctaca acgttcagat gatgctggtg gcctacggcg
    22501 ataatacgat tcagcgcttc atcattaatc acgctgcaat cttgcggatt cagatacacg
    22561 cgatcggtac ggtcagggaa ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca
    22621 ttcactttat caatgaagcg atcgcccagt ccactgacgc ttaccttagt gatatcaccc
    22681 acgttaaagt aggtatgcag ggcagaggtg gtttcaaatt cgccgtgtgc ttcaagatcg
    22741 atttcacagg ctttacccac gcggaaatgc gctaatagcg taaagtcgtg cggccagaat
    22801 tttttcgtct cttcgctttg tgtcaattca aaagtcagcg ctacgccatc agcatcttca
    22861 cgatgtgatt tcagcgtcca cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt
    22921 tgtgcagccg gaccaaacca cggccagcaa accggtacgc caccgcgaat agcgacgcca
    22981 tttttgaacg gtgtgttatt gctcaaccac agaacttctt cttcacctgc aggtttccat
    23041 gagagaaggt gtgcgccctg taatgcaaaa gaggctttta cctggggatg atcgaccaca
    23101 atgaggtcca gttcatccag tttacgacgg gagaggacag gggagatttg ttcgatgacc
    23161 ggaagggcaa aaattttctt aatcatgacg cagtccttta acttcatttt atc
//
LOCUS       DAPOZO010000078        23035 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPOZO010000078 DAPOZO010000000
VERSION     DAPOZO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.078"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..808)
                     /gene="fkpA"
                     /locus_tag="ROI21_003320"
     CDS             complement(<1..808)
                     /gene="fkpA"
                     /locus_tag="ROI21_003320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDX5112203.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAK"
     gene            1029..1247
                     /locus_tag="ROI21_003321"
     CDS             1029..1247
                     /locus_tag="ROI21_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDX5112204.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(1297..1887)
                     /gene="slyD"
                     /locus_tag="ROI21_003322"
     CDS             complement(1297..1887)
                     /gene="slyD"
                     /locus_tag="ROI21_003322"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDX5112205.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(1982..2182)
                     /locus_tag="ROI21_003323"
     CDS             complement(1982..2182)
                     /locus_tag="ROI21_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDX5112206.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(2192..3997)
                     /gene="kefB"
                     /locus_tag="ROI21_003324"
     CDS             complement(2192..3997)
                     /gene="kefB"
                     /locus_tag="ROI21_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDX5112207.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVIAVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFHGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEVE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(3997..4548)
                     /gene="kefG"
                     /locus_tag="ROI21_003325"
     CDS             complement(3997..4548)
                     /gene="kefG"
                     /locus_tag="ROI21_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDX5112208.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            4679..6592
                     /locus_tag="ROI21_003326"
     CDS             4679..6592
                     /locus_tag="ROI21_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5112209.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELEPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            6592..7614
                     /locus_tag="ROI21_003327"
     CDS             6592..7614
                     /locus_tag="ROI21_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDX5112210.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQRESGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            7608..7826
                     /locus_tag="ROI21_003328"
     CDS             7608..7826
                     /locus_tag="ROI21_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDX5112211.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            7880..8749
                     /locus_tag="ROI21_003329"
     CDS             7880..8749
                     /locus_tag="ROI21_003329"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDX5112212.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(8804..9208)
                     /locus_tag="ROI21_003330"
     CDS             complement(8804..9208)
                     /locus_tag="ROI21_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDX5112213.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            9510..10142
                     /gene="crp"
                     /locus_tag="ROI21_003331"
     CDS             9510..10142
                     /gene="crp"
                     /locus_tag="ROI21_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDX5112214.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            10193..12283
                     /locus_tag="ROI21_003332"
     CDS             10193..12283
                     /locus_tag="ROI21_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDX5112215.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12350..13570)
                     /gene="argD/dapC"
                     /locus_tag="ROI21_003333"
     CDS             complement(12350..13570)
                     /gene="argD/dapC"
                     /locus_tag="ROI21_003333"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDX5112216.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDIHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVIGA"
     gene            complement(13656..14219)
                     /gene="pabA"
                     /locus_tag="ROI21_003334"
     CDS             complement(13656..14219)
                     /gene="pabA"
                     /locus_tag="ROI21_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDX5112217.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGCLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPVCFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(14251..14853)
                     /locus_tag="ROI21_003335"
     CDS             complement(14251..14853)
                     /locus_tag="ROI21_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDX5112218.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(14843..15010)
                     /locus_tag="ROI21_003336"
     CDS             complement(14843..15010)
                     /locus_tag="ROI21_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDX5112219.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(15115..15687)
                     /gene="ppiA"
                     /locus_tag="ROI21_003337"
                     /gene_synonym="rotA"
     CDS             complement(15115..15687)
                     /gene="ppiA"
                     /locus_tag="ROI21_003337"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDX5112220.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            15958..17139
                     /gene="tsgA"
                     /locus_tag="ROI21_003338"
     CDS             15958..17139
                     /gene="tsgA"
                     /locus_tag="ROI21_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDX5112221.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            17401..19944
                     /gene="nirB"
                     /locus_tag="ROI21_003339"
     CDS             17401..19944
                     /gene="nirB"
                     /locus_tag="ROI21_003339"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDX5112222.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            19941..20267
                     /gene="nirD"
                     /locus_tag="ROI21_003340"
     CDS             19941..20267
                     /gene="nirD"
                     /locus_tag="ROI21_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDX5112223.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            20393..21199
                     /gene="nirC"
                     /locus_tag="ROI21_003341"
     CDS             20393..21199
                     /gene="nirC"
                     /locus_tag="ROI21_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDX5112224.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            21218..22591
                     /gene="cobA"
                     /locus_tag="ROI21_003342"
     CDS             21218..22591
                     /gene="cobA"
                     /locus_tag="ROI21_003342"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDX5112225.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            22838..23005
                     /locus_tag="ROI21_003343"
     CDS             22838..23005
                     /locus_tag="ROI21_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDX5112226.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         5557 a         6245 c         5851 g         5382 t
ORIGIN      
        1 ttttagcaga atctgcggct ttcgcatcag cttccggctt tgcatcagcc ttcggcgctg
       61 gtttcacatc cagcagctct acgtcaaaca ccagggtaga attcggtggg atccccggaa
      121 cacccgcttt gccgtaagcc agttctggtg gaataaccag tttgatctta ccgcctttct
      181 tgatgttctt cagaccttct gtccaacccg gaataacacc gtccagacgg aaagagagcg
      241 gttcaccacg ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta cctttgtagt
      301 tcacgacaac agtatcgctg tctttcggtg cttcgccttt accggcttcg actacctgat
      361 aaaccagacc agttgaagag gttttcacac ctttctcttt ggcaaatttc tcgcggtact
      421 ctttaccttt cgcttcgtta tctgccgcgt ctttttccat cttcgcctga gcagaagact
      481 tcacgcgagc ttcgaatgct tgcagtgtct gttcgatctc ttggtcggag agtttgctct
      541 tatcagcaaa tgcatcctga acaccagcga tcagctgatc tttatccagt ttgatgccca
      601 gtttttcttg ttctttaaga gagttttcca tgtaacgacc cagcgaagca cccagtgcat
      661 aagctgattt ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt gtagcaggtt
      721 ttgcagcttc agcagcaaaa gtgatcggtg catgcagggc aacggccatt gtggtcgcca
      781 gcagcgttac tttaaacagt gatttcatcc atatctccag gatcggggca tctcacccca
      841 gggttaacta ttatcagaag ggtactataa agcgttgtcg aacaaatcta catacagaca
      901 cgccctatta tcatctattt tcagactctt tttgtttaaa ttagtttcga tgaccgcgaa
      961 atgagtgctg tctcgggcag caaagttaag tagaatccgc ggcgaccatt cgacaaaaga
     1021 ggtgaatcat gcaggattta tcattggaag cacgcctggc agagctggag agccgactgg
     1081 cttttcagga aatcaccatt gaagaactga acgtcacggt gaccgctcat gaaatggaga
     1141 tggcgaaact gcgcgatcat ctgcgtctgc tgaccgagaa gctaaaagcc agccagccgt
     1201 cgaacatcgc gtcgcaggct gaagaaacgc caccgccaca ttattgaggc gtaaaaaaag
     1261 cggggattcc cccgcttttt tgtcactttt tcggtattag tggcaaccgc aaccgccgtt
     1321 acctttaccg ccgcagcagc cttcgccacc gtgttcatga ccgtgatcgt ggccatgacc
     1381 accgcagcaa ccgtcgtggt cgtgatcgtg gtgatgatcg tgcgcaccgt gaacgtggcc
     1441 atgagccagt tcttcttcag tcgcttcgcg aatcgccaca acttcaacgt tgaatttcag
     1501 gttctgaccg gccagcatgt ggttaccatc aaccacgacg tgatcgtctt caaccgcagt
     1561 gatttcaacc ggtaccggac cctggtcagt ttcagccagg aaacgcatac ctacctgcag
     1621 ttcatcaacg cccataaata cgtctttagg aacgcgttgc accaggtttt cgtcgtactg
     1681 accgtaagcg tcgttcgcgc caacagcgac atcaaatttg tcgccaactt cgtgaccttc
     1741 cagcgccgtt tccaggccag agatcaggga accgtgacca tgcaggtagt ccagcggcgc
     1801 actcaccgga gactcatcaa ccaacacacc gtcttctgta cgtacctgat aggccaggct
     1861 gaccaccagg tcttttgcta ctttcatgat atctcctgag catgggaaga atagtggcgc
     1921 agattgtagc ggaattctgc agccgtgtac tcactagctt aaaaaaactc ggcgcatatc
     1981 gctagtccgg atgaaaaatc ccgatcactt gctcatcttt gcgaacgtga tcgcgggctt
     2041 ctttgtccgc ttctcgcatc tgatgtccgc acttaacaca ttcaacaata tcaatattat
     2101 tttcgcgcca catcgccatt gaatcctgcg cctgacaggc cgggcatttt gcgcccgcaa
     2161 taaaacgttt tcggattgcc atctttaccc tctactcaaa ttcatcccag ccgtccagct
     2221 ggcgtcgttc ttgttgcatt tcacgctgga aaatctcttc cagttcgcgt cgggcttccc
     2281 tggcgcgaga aatttgtacg gtatcggcat gcattgggat gagctctcgc agcatccgca
     2341 tatccaggcg gcgaaaatgc agttgcgccc gctgtgcctg atgcggatgc atgccaagcg
     2401 tgaccagcgt cttgcgcccc agctctaacg cactggagaa tgtttcacgg gaaaactgcg
     2461 tcacccctgc ctgtaataac tcatgcgctt ccacacgtcc gcgcgctcgc gcaagaatat
     2521 gcaaatgcgg aaagtgctgc tggcatattt ccaccagttt catggtgtct tccggctcgt
     2581 tacaggtaat gacgatagac tcagcggcct ctgcacccgc agaacgtaac agatcgacct
     2641 gcgtggcgtc gccgtaataa actttgtagc catatttgcg catcaggtta acggcgctga
     2701 tatcccgctc cagcacagta atgcgcattt tatttgccat cagcaagcga ccaatcacct
     2761 gaccaaaacg cccaaagccc acgacaatga cctgcggttt atcatcgttg acccacggtt
     2821 tttcgacttc ttcttccggt ccgttaaact ggcgggatag ccatttatct accagcttca
     2881 tcagcagcgg cgtggtcatc atggaaagcg tcaccgtcac cagtaacagc gccatctggt
     2941 cgccatggaa taagcgttgt gaagaagcgg tagaaaagag cacaaaggca aactcaccgc
     3001 cctgactcaa cacgccagca aactgcatcc gctctgagct acgcacgcca tacaatcgcg
     3061 ccagcagata cagcacgaga attttcaccg ccaccagcac aaccacagct atcactaccc
     3121 acaacagatg ggtataaagc accccgaggt tgagtgacat gccgacagag ataaagaaca
     3181 aaccgagcag caagcctttg aagggatcga tagccgtttc cagttcatga cgatattcac
     3241 tttccgccag cagcacgccc gcaataaacg taccgagcgc catcgacagc cccagcgcat
     3301 ccataaacaa tgcggaaccc aacaccagca gcagcgtcgc ggcggtgaac acttcccgca
     3361 caccagaagc tgcaataaaa cggaataccg gacgcagtaa atagcgcccg ccaatcagca
     3421 tgccgacaaa cgccagcacc ttcatgccga tcttcatcca gtcgaaatgt tcgtctgccg
     3481 aacccgccaa caacggcact aacgccagtg ccgggatcac cgccagatcc tgaaacagca
     3541 gtaccgagaa acctaactgc ccggattcgc tgcgattcat ccctttctca cgcatcaatt
     3601 gcaacgccat tgcagttgaa gacatcgcaa ggccaatgcc accgaccacc gccgcctgcc
     3661 aggcgaaatc cgtcagcatc aataatcccg ccagcaacgc cgcgcttaac agcacctgtg
     3721 ccgcacctac gccaaaaatc gaacgccgca attgccaaag cttggaggga ttcaactcca
     3781 ggccgatgat aaacatcagg aacaccacgc cgagttccga aaagtggagg atctcatcga
     3841 cgtcgctaat aaaacccagc ccccacgggc caattgcaat ccctgccagc aaatatccca
     3901 acacagcgcc aatacccagc cgcgatgcca gcggcaccgc agccaccgcc gcgaagagaa
     3961 acaacactcc tgcgagtaaa aaatcggaac cttccatcag cggcctcctg gagacagcgg
     4021 atttgccagc cagtcaccgt aggctctggc gtgactcgcc agctcctgtg cgctttgccg
     4081 tctcgcccag taaataatga tgggactcaa ccaatgcatc cggcacatgc ccgccgctag
     4141 ttcaaagggg cgcagaacat cgctcatcgg gtagcgattc aaggcgtcat aacggtaagc
     4201 actttccggc tcgccggtgg taatcacgct acgccagtac tttcccgcca gttggtttcc
     4261 tcccggcccg ctggcaaaac cacggcttaa tacccggtcc agccactctt tcagtagcgc
     4321 cgggcagcta taggtataaa gaggatgctg aaagacaatc acctcgtgct cgcgcagtaa
     4381 tgcctgctca cgggggatat caataaaaaa atcgggatag tgcgcgtaaa ggtcgtgcac
     4441 ggtaacattg ctgagctgcg tggccggttt aagcagtacc cggtttgcca ccgagtcctg
     4501 agattccgga tgggcataca gcagcaaaac tttcgctggc tgagacatca tccccctccc
     4561 ggtatggttt ttgtgtatag tcgctgtttt gggctaccat tgcgcccggt gcggcagctc
     4621 gcccatacat tacattatca taatgataag ttaacatagt ctgaacatac ggcaccttat
     4681 gattgttttc tcctcgttac aaattcgtcg cggcgtgcgc gtcctgctgg ataatgccac
     4741 cgccaccatc aaccccgggc agaaagtcgg cctggtgggt aaaaacggct gtggtaaatc
     4801 taccctgctg gcattgctga aaaatgaaat cagcgccgac ggcggcagct acacctttcc
     4861 gggaagctgg caactggcgt gggtgaatca ggaaacgccg gcgttaccgc aagcggcgct
     4921 ggaatatgtc attgacggcg accgtgaata tcgtcaactg gaagcgcagc tacacgacgc
     4981 caacgaacgt aacgacgggc acgccatcgc caccattcat ggcaagctgg atgctatcga
     5041 cgcatggagt attcgctccc gtgccgccag cctgctgcac ggcctcggtt tcagcaatga
     5101 acaactggag cgcccggtga gtgatttctc cggtggctgg cgtatgcgtc ttaaccttgc
     5161 ccaggcgctg atttgccgtt cagacttgct gctgctcgac gaaccgacta accacctcga
     5221 tctcgatgcc gttatctggc tggaaaaatg gctgaagagc tatcagggca cgctgatcct
     5281 gatctctcac gaccgcgact tcctcgatcc gatcgttgat aaaattattc atatcgaaca
     5341 acaaagcatg ttcgagtaca ccggcaacta cagttcgttt gaagtgcagc gcgccacccg
     5401 tctggcgcag caacaagcga tgtacgaaag ccagcaggaa cgcgtggcgc atctgcaaag
     5461 ttatatcgac cgtttccgtg ccaaagccac caaagcgaag caggcccaga gccgcattaa
     5521 gatgctggag cgtatggagc tgattgcccc cgcgcacgtc gacaacccgt tccgttttag
     5581 cttccgcgcg ccggaaagcc tgccaaatcc gttactgaag atggaaaagg tcagcgcagg
     5641 ctatggcgat cgcattattc tcgactcgat taaactgaac ctggtgcccg gctcgcgcat
     5701 tggtctgtta ggccgcaatg gcgcgggtaa atcgacatta atcaaactgt tagccggtga
     5761 acttgagcca gtcagcggtg aaattggtct ggcgaaaggg atcaagctcg gctacttcgc
     5821 ccagcatcaa ctggaatacc tgcgcgccga cgaatcgcct attcaacatc tggcacgttt
     5881 agcgccgcag gagctggagc aaaaactgcg tgattacctc ggcggctttg gtttccaggg
     5941 cgataaagtg acggaagaaa cgcgccgctt ctccggtggg gaaaaagccc gcctggtgct
     6001 ggcattaatt gtctggcagc gtccgaatct gctgctgctc gacgaaccga ctaaccacct
     6061 tgacctcgac atgcgtcagg cactgactga agcattaatc gagttcgaag gcgcgctggt
     6121 tgtcgtctcg cacgaccgtc atttgctgcg ttccaccact gacgatctct acctggttca
     6181 cgatcgtaaa gtcgaaccgt tcgacggcga tctggaagat tatcaacagt ggttgagcga
     6241 cgtacaaaaa caggaaaacc agaccgacga agcgccaaaa gagaacgcaa acagcgccca
     6301 ggcacgtaaa gatcagaagc gtcgggaagc ggagctgcgt gcgcaaaccc aaccgctgcg
     6361 taaagagatc gcccgtctgg aaaaagagat ggaaaagctg aacgcacagc tggcacaggc
     6421 ggaagagaaa ctcggtgaca gcgaactgta cgatcaaagc cgtaaagcag aactgaccgc
     6481 ctgcctgcaa cagcaagcca gcgccaaatc cggcctggaa gagtgcgaaa tggcgtggct
     6541 ggaagcccag gagcagcttg agcagatgct gctggaaggc caaagcaact gatggcgcag
     6601 ataacgacga ccgatgccaa tgaattcagc agcagtgctg aattcacccc tatgcgcggc
     6661 tttagcaatt gtcatctaca aaccatgctg ccgcgtctgt ttcgtcgcaa ggtgaaattc
     6721 accccgcact ggcagcgact ggagttgccc gacggcgatt ttgtcgatct cgcgtggagt
     6781 gaagaccctg cacaggcgcg tcataaaccg cgtttagtgg tatttcacgg gctggaaggc
     6841 agtctcaata gcccttacgc tcacggtctg gttgaggcgg cgcaaaagcg cggctggctg
     6901 ggcgtggtga tgcattttcg cggatgcagc ggtgaaccaa accgtatgca ccgcatttac
     6961 cattcgggcg aaaccgaaga cgccagttgg tttttacgct ggctgcaacg cgagtctggt
     7021 catgcgccaa cggctgccgt cggctattcg ctcggcggta atatgctggc ctgtttgctg
     7081 gcaaaagaag gcaatgatct cccgattgat gcggcggtga ttgtctctgc gccgtttatg
     7141 ctggaagcct gtagctatca tatggaaaag ggcttttccc gcgtctatca gcgttacttg
     7201 ctgaacctgt taaaagccaa tgccgcacgc aagctggctg cctaccccgg aacgctgccg
     7261 attaatctcg cgcagttaaa atcggtacgt cgcatccgtg aatttgacga tctcatcacc
     7321 gccagaattc acggctacgc cgacgctatc gactattatc gtcagtgtag cgccatgccg
     7381 atgctgaacc ggatcgccaa accgacgctg attattcacg ccaaagacga tccgtttatg
     7441 gatcatcagg tgatcccgaa accggaaagt ctccccccgc aggtggagta tcaactgact
     7501 gaacatggcg gtcatgttgg ctttattggc ggtacattac ttcatccgca aatgtggctg
     7561 gagtcacgca ttcctgactg gttaacaacg tatctggagg cgaaatcatg ttgattccgt
     7621 ggcaagacct ctcccccgaa acgctggaaa atttaattga aagctttgtg ttacgtgaag
     7681 gcaccgatta tggtgaacat gagcgtacac ttgaacagaa agtcgccgac gtcaaacgcc
     7741 agctacagtg cggagaagcg gtgctggtat ggtcggagct gcacgaaacg gtcaatatta
     7801 tgccgcgcag tcagtttcgc gaataacctg caaccgtcgg cggacgagat aataatgata
     7861 gctaagtcat ggagttacta tgtctgccaa acatccggtc attgcggtaa caggatccag
     7921 cggcgcgggg accaccacca ccagcctcgc gtttcgtaaa atattcgcgc agttaaatct
     7981 gcatgcagct gaggtggaag gcgacagttt tcaccgttac acccgcccgg aaatggacat
     8041 ggcgatccgc aaagcgcgcg acgccgggcg gcatatcagc tacttcggcc ccgaagcgaa
     8101 cgactttggc ctgctggaac aaaccttcat tgaatatggt cagagcggca aagggaaatc
     8161 tcgcaaatat ctgcatacct acgacgaagc cgtaccgtgg aatcaggtac cggggacatt
     8221 caccccatgg caacctttac cggaacccac tgatgtactg ttttatgaag gtttacacgg
     8281 cggcgtcgtc acgccacagc ataacgttgc gcagcatgtg gacttactgg tcggcgtggt
     8341 gcctatcgtt aaccttgagt ggattcaaaa actgatccgc gacaccagcg agcgcgggca
     8401 ctcacgagaa gcagtgatgg actcggtagt gcgttcaatg gaagactata tcaactacat
     8461 cacaccgcag ttttcccgca cccatcttaa cttccagcgc gttcccaccg tcgacacttc
     8521 aaacccgttc gcggcaaaag gtatcccgtc gctggatgaa agctttgtgg tgatccattt
     8581 tcgtaatctg gaagggatcg attttccctg gctgctggcg atgttgcaag gctcattcat
     8641 ttcccacatc aatacgttag tggtgcctgg cggcaaaatg ggtctggcaa tggaattaat
     8701 tatgctgccg ctggtgcaac gattgatgga agggaagaaa atcgagtaac tctgctatta
     8761 cgccggataa aatactatcc ggcttcacaa cgggatagtt aaatcacgcg gcaaccactt
     8821 catacgagtg agtaatattc accgcttttt ccagcatcaa cgccactgaa caatatttct
     8881 cggcagagag atctaccgca cgcgcaaccg ctgcgtcttt caggtcgcga ccggtgacga
     8941 taaaatgcag attaatgtgc gtaaacaggc gtggtgcctc ttcgcggcgt tcagaggtca
     9001 attttacttc acaatcgacc acatcctgac gccctttttg cagaatagaa accacatcga
     9061 tggcactgca accacccgcc gccatcaaca ccatttccat cggacttggt gctttatcgc
     9121 ctgagttgcc gtccattaaa atctggtgac cagaggcgga ttcgcccaga aaagttaacc
     9181 cttcgaccca cttcactcgc gcttgcattt ttgctactcc actgcgtcaa ttttcctgac
     9241 agagtacgcg tactaaccaa atcgcgcaac ggaaggcgac ctgggtcatg ctgaagcgag
     9301 acaccaggag acacaaagcg aaagctatgc taaaacagtc aggatgctac agtaatacat
     9361 tgatgtactg catgtatgca aaggacgtca cattaccgtg cagtacagtt gatagcccct
     9421 tcccaggtag cgggaagcat atttcggcaa tccagagaca gcggcgttat ctggctctgg
     9481 agaaagctta taacagagga taaccgcgca tggtgcttgg caaaccgcaa acagacccga
     9541 ctctcgaatg gttcttgtct cattgccaca ttcataagta cccatccaag agcacgctta
     9601 ttcaccaggg tgaaaaagcg gaaacgctgt actacatcgt taaaggctct gtggcagtgc
     9661 tgatcaaaga cgaagagggt aaagaaatga tcctctccta tctgaatcag ggtgatttta
     9721 ttggcgaact gggcctgttt gaagagggcc aggaacgtag cgcatgggta cgtgcgaaaa
     9781 ccgcctgtga agtggctgaa atttcgtaca aaaaatttcg ccaattgatt caggtaaacc
     9841 cggacattct gatgcgtctg tctgcacaga tggcgcgtcg cctgcaagtc acttcagaga
     9901 aagtgggcaa cctggcgttc ctcgacgtga cgggccgcat tgcacagact ctgctgaacc
     9961 tggcaaaaca accggatgct atgactcacc cggacggtat gcaaatcaaa attacccgtc
    10021 aggaaattgg tcagatagtc ggctgttctc gtgaaaccgt gggacgcatt ctgaagatgc
    10081 tggaagatca gaacctgatc tccgcacacg gtaaaaccat cgtcgtttac ggcactcgtt
    10141 aatcccgtcg gagtggcgcg ttactgagta gcgcgccatt ttgtttcccc cgatgtggcg
    10201 cagactgatt tatcaccccg atatcaacta tgcactgcga caaacgctgg tgctatgttt
    10261 gcccgtggcc gttgggttaa tgcttggcga attacgattc ggtctgctct tctccctcgt
    10321 tcctgcctgt tgcaatattg cgggccttga tacgcctcat aaacgttttt tcaaacgctt
    10381 aatcattggt gcgtcgctgt ttgccacctg tagcttgctg acacagcttc tactggcaaa
    10441 agatgttccc ctgccctttt tgctgaccgg attaacgctg gtacttggcg tcactgctga
    10501 gctggggcca ttgcacgcaa aattgcttcc cgcatcgctg ctcgccgcca tttttaccct
    10561 cagtctggcg ggatacatgc cggtctggga accgttgctc atctatgcgt tgggcactct
    10621 ctggtacgga ttgtttaact ggttttggtt ctggatctgg cgcgaacaac cgctgcgcga
    10681 gtcactaagt ctgctgtacc gtgaactggc agattattgt gaagccaaat acagcctgct
    10741 tacccagcac accgaccctg aaaaagcgct gccgccgctg ctggtgcgcc agcaaaaagc
    10801 ggtcgatcta attacccagt gctatcagca aatgcatatg ctttccgcgc aaaataatac
    10861 cgattacaag cggatgctgc gtattttcca ggaggcgctg gatttgcagg aacatatttc
    10921 ggtcagtttg catcagccgg aagaggtgca aaaactggtc gagcgtagcc atgcggagga
    10981 agttatccgc tggaatgcgc aaaccgtcgc cgctcgcctg cgcgtgctgg ctgatgacat
    11041 tctttaccat cgcctgccga cgcgttttac gatggaaaag caaattggcg cactggaaaa
    11101 aatcgcccgc cagcatccgg ataatccggt tgggcaattc tgctattggc acttcagccg
    11161 cattgcccgc gtgctgcgca cccaaaaacc gctctatgcg cgtgacttac tggccgataa
    11221 acagcggcga atgccgttac ttccggcgct gaaaagttat ctgtcactaa agtctccggc
    11281 gctacgcaat gccggacgac tcagtgtgat gttaagcgtt gccagcctga tgggcaccgc
    11341 gctacatctg ccgaagtcgt actggatcct gatgacggta ttgctggtga cgcaaaatgg
    11401 ctatggcgca acccgtctga ggattgtgaa tcgctccgtg ggaaccgtgg ttgggttaat
    11461 cattgcgggc gtggcgctgc actttaaaat tcccgaaggt tacaccctga ccttgatgct
    11521 gattaccacc ctcgccagct acctgatatt gcgcaaaaac tacggctggg cgacggtcgg
    11581 ttttaccatt accgcagtgt ataccctgca actgttgtgg ctgaacggtg agcagtacat
    11641 ccttccacgt cttatcgata ccattattgg ttgtttaatt gctttcggcg gtactgtctg
    11701 gctgtggccg cagtggcaga gcgggttatt gcgtaagaac gcccatgacg ctttagaagc
    11761 ctatcaggaa gcgattcgct tgattctgag cgaggacccg caacctacgc cactggcctg
    11821 gcagcgaatg cgggtaaatc aggcgcataa cactctgtat aactcattga atcaggcgat
    11881 gcaggaaccg gcgtttaaca gccattatct ggcagatatg aaactgtggg taacgcacag
    11941 ccagtttatt gttgagcata ttaatgccat gaccacgctg gcgcgggaac accgggcatt
    12001 gccacctgaa ctggcacaag agtatttaca gtcttgtgaa atcgccattc agcgttgtca
    12061 gcagcgactg gagtatgacg aaccgggtag ttctggcgat gccaatatca tggatgcgcc
    12121 ggagatgcag ccgcacgaag gcgcggcagg tacgctggag cagcatttac agcgggttat
    12181 tggtcatctg aacaccatgc acaccatttc gtcgatggca tggcgtcagc gaccgcatca
    12241 cgggatttgg ctgagtcgca agttgcggga ttcgaaggcg taatgcaggc taaaccatcg
    12301 gatacagcgt tcgcgccgca tccgacatct tttgcctgat gcgtcgtatt tacgccccaa
    12361 ttaccttcgc cactgcgtgg gcgaaacgtt gcatcccttc atcgatatcc gcatcttcca
    12421 ccaccagcga cggtgcaaaa cgcatcacat ccggtccggc attcagcacc attacgccag
    12481 cctctgcgcc cgcatacagg aaatcacgcg cccgaccttt gtactgtggt ttcagctccg
    12541 cgccaattaa cagccccata ccgcgaatat cgctaaacac atcgtactgc tgatcgatct
    12601 tctgcaaatg gtcaacaaaa cgctggcgtt tcgcctgaat gccttccagc acttcagggg
    12661 tattgatgat atcaaacgcc gcgcccgcta ctgcacaggc cagaggatta ccgccgtagg
    12721 tggaaccgtg agaaccagga tgaaatgcag aagcaatttc cgccgtggtc agcatggcgc
    12781 taatcgggaa gccgccgcct aacgctttcg cagaggtcag aatatccggc gtcacgccgt
    12841 agtgcatgta agcaaacaga tcgccggtcc gccccatccc gcactgcact tcatcaaaca
    12901 ctaataatgc ctgatgttga tcgcacagct cgcgcaagcc ctgcaaaaac tctggcgtcg
    12961 ctgccgtcac gccgccctcg ccctggatcg gctcaaccac caccgcacag gtgtgatcat
    13021 ccatcaccgc tttcactgcg tggatatcgt taaaaggcac gtggatgatg tctgccggtt
    13081 ttggcccaaa gccgtcggaa tattttggct gcccacccac cgaaacggta aacagcgagc
    13141 gaccatgaaa agcgttatgg aaggcaataa ttttggtttt gaacgggcta tgacgcacac
    13201 aggcgtaatg acgcgccagt ttgaaggcgg tttcgttagc ttccgtgccg gagttcataa
    13261 acaccacgcg ttcggcaaac gtcgcctcaa tcagtttacg cccaagacgc agcgccggtt
    13321 cattggtgaa aacgttactg atatgccaca gagtttcgcc ctgggttttt aacgcattca
    13381 ccagcgcagg atggcaatgg cccaacgccg taactgcaat gccacccgcg aaatcgacat
    13441 actccttgcc ttgctgatcc cagattcggc tgccctgacc ttttaccgga ataaactctg
    13501 ccggagcata aatcggcagg atcacttcat cgaaagtcgc gcgtgtaatt gctgtttgtt
    13561 caattgccat ctcatgatca ccctgttacg cataaaccaa tatgaaatta taatcacaaa
    13621 atatgcataa aaaatcactt aatagcaatc agaaatcagc gatgcaggaa attagccagc
    13681 agttgatgtc cttgttcgct gagaatactt tccgggtgga actgcacacc ttccagatcc
    13741 cactggcgat ggcgaatccc cataatctcg cgggtttcgc tccaggccgt cacttcaaag
    13801 cacacaggta atgagtcagg ttccaccacc agcgaatggt agcgtgtcac ggtaagtgga
    13861 tttgccagcc ccttaaatac gcccacaccg ttatgtgtaa tcggcgaggt tttgccgtgc
    13921 atgacctttg cggcgcgcac aactttaccg ccaaatgctt gcgccattgc ctgatgaccg
    13981 aggcaaacgc caagaatcgg caagcacccg gcatagtggc gaataacgtc aagggagatc
    14041 ccggcttcat ctggcgtaca gggtccgggt gagatgacaa ttttttgtgg tttaagggcg
    14101 tcgatatccg ccagcgtcaa cgcatcgttg cgcttaacca gcacatccgc ccccagttca
    14161 caaaagtact gatagaggtt ccaggtaaaa gaatcgtagt tatctataag caggatcatg
    14221 gcggctccgg tacgaaagaa ccgcgctatt ttactcagat tccccggctt cgcttaccac
    14281 tttgctaaat atcatctgca gcgcggtgag atcccccatt gccccactct gatttgcctg
    14341 attccaggcc tctttttcga taccctgcca gctcagttga tatccggcat gaatcgccag
    14401 ctgctcgaag aagatccgct gtgccaaacc acttcccacc cggaagggat gcagcacgtt
    14461 gatttcacaa tagtaatgcg ccagccgctc gacgaacttc gctttctcca ggccaaccag
    14521 ataaccttct tcctccagat cctgcatcag ggcattgccc tctttttcga tataagcaaa
    14581 gtggcagaac ggcgtatcac cctgataaat atcaacttca cgcagttgcc ccgcccagtc
    14641 aaaaatatcc tgatacagct ggcgatggat agtgcgcaaa tgcggtaaac cgcgcaccag
    14701 cggaccaagc tcaatggtcg cagcacgcag cgccgtcatt tcgtaagcgg cctgttccag
    14761 ccgctgttgc tggcggatgt tcagccggtt acgcatgata tcaaggccag gataaagata
    14821 cggatcgcgc ccttcgccga atttatcgct catagtgcct cctcagctct tcaaggcgcg
    14881 ctaaagcctc tgcggcagta agagtgacta aaggcatctc gacgccttca agacggcgac
    14941 tggcctggaa attacgatta cgctgaagct cccagagacg ggacttttgc ttatcggtga
    15001 gtttcttcac ttgatgcctc cctgaacgtg tccatttgcc acaagtataa gcggcaaatc
    15061 tgggttacgc cgggagagca gaagcgcggg caagattgcc cgcgcgagaa atcattacgg
    15121 caggacttta gcggaaagga taactaccgg ttttgacggc acattctggt acggaccaac
    15181 gtcatgagtc ggcacctggg aaatcttatc ggcaacgtcc atgcctttca ccactttacc
    15241 aaataccgcg taaccgaaat cacgctgacc atggtcaagg aaggcgttat cggcaacgtt
    15301 gataaagaac tggctggtgg cgctgtcttt gtcagcggta cgcgccatcg cgatggtgcc
    15361 acgcgtgttg cgcaggccgt tatcggcttc atttttgatt ggcgggtttg gttttttctg
    15421 ctgcatctgc tcggtgaaac cgccgccctg aatcataaag ccaggaatga cgcggtgaaa
    15481 ggtagtgttg ttataaaaac cgctgttcac ataatcgaca aagttttgca cagacactgg
    15541 cgctttttgt ttatccagct ccagttcgat gttaccagct gaggttgtca acaatacgtg
    15601 cgggtcccct ttcgctgcca ttgctgcggg agaaagagca gaaagagcga aaacagcagc
    15661 catcgccgcc agggtcgatt tgaacatgag atttccttaa caaacagagc agaaaaaaag
    15721 caagtgaaga gattctaaat agccttcaag aacaaggcca tccctttacc taattttacg
    15781 tatctgaaat atatgtaacc tgacgatcac tttttattcc gtgatcaaaa tcacctctta
    15841 aaatgcaatt tagcaaccga ttgcaataaa acatttaaac agatcacaaa atcacctaaa
    15901 atcgcccgct cgcggcatat cccgctatgc cattttattt ttgaacgcag gccaatcatg
    15961 actaacagca atcgcatcaa gctcacatgg attagctttc tctcctacgc actgaccggt
    16021 gcgttggtta ttgtcaccgg gatggtgatg ggaaatatcg ccgattattt caatctgcct
    16081 gtttccagta tgagtaacac cttcaccttc ctcaacgccg gcattttaat ctctatcttc
    16141 ctcaacgcct ggctgatgga aatcgtcccg ttgaaaacgc agttacgttt tggctttctc
    16201 ctgatggtgc tggcggttgc cggtttgatg ttcagccaca gcctggcgct gttctcagcg
    16261 gcaatgttca ttctcggggt ggtcagcggc attactatgt cgattggtac attcctgata
    16321 acacaaatgt atgaagggcg tcagcgcggt tcccgcctgt tatttactga ctccttcttc
    16381 agtatggctg ggatgatttt cccaatgatc gccgcgtttc tactggcgcg cagcattgag
    16441 tggtactggg tttatgcctg catcgggctg gtgtatgtcg ctatttttat tctgaccttc
    16501 ggctgtgagt tcccggcact gggcaaacat gcaccaaaaa cggatgctcc ggtagcgaaa
    16561 gaaaagtggg ggatcggcgt actgtttctc tcagttgcgg cgctgtgcta catcctcggt
    16621 cagttaggtt ttatctcctg ggtgcctgag tatgccaaag gcctgggcat gagcctgaat
    16681 gatgctggca cgctggtgag taacttctgg atgtcatata tggtcggcat gtgggcgttc
    16741 agctttattc ttcgcttctt tgatttgcaa cgtattctga ccgtactggc tggtctggct
    16801 gcgattctga tgtacgtctt taacaccgga acaccagcac atatggcttg gtcaattctc
    16861 gctctgggct tcttctccag cgcgatctat accaccatca tcaccctggg ttcacagcag
    16921 accaaagtac cgtcgccaaa actggttaac tttgtcctga cctgcggaac catcggtact
    16981 atgttgacct ttgtggttac cggcccgatt gttgaacata gcggtccgca ggcggcactg
    17041 cttacagcaa acggtctgta cgctgtcgtc tttgtgatgt gcttcctgtt aggtttcgtc
    17101 agccgtcacc gtcagcataa caccctgacc tctcattaat tgctcatgcc gggcggcgct
    17161 atcgtcgtcc ggccttttcc tctcttaccc tgctacgtgc atctatttct ataaacccgt
    17221 tcaatttgtc tgttttttgc acaaacatga aatgtcagac aattccgtga cttaagaaaa
    17281 tttatacaaa tcagcaatat accacttaag gagtatataa aggtgaattt gatttacatc
    17341 aataagcggg gttgctgaat cgttaaggta ggcggtaata gaaaagaaat cgaggcaaaa
    17401 atgagcaaag tcagactcgc aattatcggt aacggtatgg tcggccatcg ctttatcgaa
    17461 gatcttcttg ataaatctga tgcggccaac tttgatatta ccgttttctg tgaagaaccg
    17521 cgcatcgctt atgaccgcgt acacctctcg tcttacttct ctcaccacac cgccgaagag
    17581 ctgtcgctgg tgcgcgaagg cttctacgag aaacacggca tcaaagttct ggtcggcgaa
    17641 cgcgctatca ccatcaaccg tcaggagaag gtgattcact ccagcgccgg gcgcaccgtt
    17701 ttttatgaca agctgatcat ggcgaccggt tcctacccgt ggatcccgcc aatcaaaggt
    17761 tctgatactc aggactgctt tgtctatcgc actattgaag acctcaacgc cattgaatcc
    17821 tgcgcccgtc gcagcaaacg cggtgccgtt gttggtggcg gcctgttagg tctggaagcc
    17881 gctggcgcgc tgaaaaactt aggtattgaa acccacgtta tcgaatttgc ccctatgctg
    17941 atggcggaac agcttgatca gatgggtggc gagcagctgc gtcgcaaaat cgaaagtatg
    18001 ggcgtgcgcg ttcacaccag caaaaacacc cttgagattg tgcaggaagg tgtcgaagcg
    18061 cgtaaaacca tgcgttttgc cgacggcagc gaactggaag tcgactttat cgtcttctct
    18121 accggtatcc gtccgcgcga taagctggca acccagtgtg gtctggacgt tgctccgcgt
    18181 gggggtattg tcattaatga ttcctgccag acttccgatc cggatatcta cgccatcggt
    18241 gaatgcgcaa gctggaacaa ccgtgtattt ggtctggtag caccaggcta caaaatggcg
    18301 caggtcgccg ttgaccatat tctcggtagc gaaaacgcct ttgaaggtgc tgaccttagc
    18361 gccaagctga aactgctggg cgtagacgta ggcggtattg gtgatgcgca cggtcgcaca
    18421 cctggcgcac gtagctacgt ttacctcgac gaaagcaaag agatctacaa acgccttatt
    18481 gtcagcgaag acaacaaaac cctgctcggt gcggtactgg tgggcgatac cagcgactac
    18541 ggcaacctgc tgcaactggt gctgaatgct atcgagctgc cggaaaaccc ggattctctg
    18601 attctgccgg cgcactctgg tagcggtaag ccgtctatcg gtgttgataa actgccggac
    18661 agcgcgcaaa tctgctcctg cttcgacgtc accaaaggcg acctgattgc tgccatcaac
    18721 aaaggctgcc acaccgttgc ggcgctgaaa gccgaaacca aagcgggtac tggctgcggt
    18781 ggctgtatcc cgctggtcac tcaggtactg aacgcggaac tggcgaaaca gggcatcgaa
    18841 gttaacaaca acctgtgcga acactttgct tattcgcgtc aggaactgtt ccatttgatc
    18901 cgcgttgaag gcattaaaac cttcgaagag ctgctggcga aacacggcaa aggctacggt
    18961 tgtgaagttt gtaaaccgac cgtcggttcg ctgctggcct cctgctggaa cgaatacatt
    19021 ctgaagccgg aacatactcc tctgcaggat tctaacgata acttcctcgc taacatccag
    19081 aaagacggca cctactcggt gatcccgcgt tctccgggcg gtgaaatcac cccggaaggg
    19141 ctgatggcag tgggtcgtat cgcgcgtgaa tttaatctct acaccaaaat caccggttcc
    19201 cagcgcctgg cgatgtttgg cgcacagaaa gacgatctgc cggaaatctg gcgtcagctg
    19261 attgaagccg gcttcgaaac cggtcatgcc tatgcgaaag cactgcgtat ggcgaaaacc
    19321 tgcgtgggta gcacctggtg ccgctacggt gttggcgaca gcgtcggcct cggcgtggaa
    19381 ctggaaaacc gctacaaagg catccgtacg ccgcacaaaa tgaagttcgg tgtctccggc
    19441 tgtacccgtg aatgttcaga agctcagggt aaagacgtgg gtattatcgc caccgaaaaa
    19501 ggctggaacc tgtacgtttg cggcaacggc ggcatgaaac cgcgtcatgc ggatctgctg
    19561 gcggcggata tcgatcgcga aacgctgatc aaatatctcg accgcttcat gatgttctac
    19621 atccgtactg ccgacaaact gacgcgtacc gcgccgtggt tagaaaacct cgaaggcggc
    19681 atcgattacc tgaaagcggt gatcattgac gacaaactgg ggctgaacgc tcatctggaa
    19741 gaagagatgg cgcgcctgcg tgaagcggta gtgtgtgagt ggactgaaac ggtcaataca
    19801 ccgtccgcgc agactcgctt caaacacttc atcaacagcg acaagcgtga cccgaacgtg
    19861 cagatggtgc cagagcgcga acagcaccgt ccggcaacgc cgtatgaacg tatcccggta
    19921 actctggtgg aggacaacgc atgagccagt ggaaagacat ctgcaaaatc gatgacatcc
    19981 tgcctgaaac cggcgtctgc gcgctgttag gtgacgagca ggtagcaatt ttccgcccgt
    20041 atcacagcga ccaggtgttt gcgatcagca acatcgaccc gttcttcgag tccagcgtgc
    20101 tgtcacgcgg gctgattgcg gaacaccagg gcgagctgtg ggtcgccagc ccgctgaaaa
    20161 aacagcgttt tcgcttaagc gacggcttgt gcatggaaga cgaacagttt tccgtcaagc
    20221 attacgacgc gcgagtgaaa gacggcgtgg tgcagctacg cggttaatgt tttaacggga
    20281 ggcgcaatgc ctcccctttt tgcatggtcc tgtaataacc ttcggtatat tgcaggacat
    20341 tttttaaact ttttgtttta ttttttgttt ttatttttta aaggataatc aaatgtttac
    20401 agacactatt aataagtgtg cggctaacgc tgcgcgcatt gcacgcctgt cggcaaataa
    20461 cccgctcggc ttctgggtca gctccgccat ggcgggcgcg tatgtgggtc ttgggatcat
    20521 cctgatcttc acgctcggta atttgctcga cccgtccgta cgtcctctgg tgatgggcgc
    20581 gacctttggt atcgccttaa cgttggtcat tattgctggt tctgagctgt tcaccggaca
    20641 caccatgttc ctcacctttg gagtgaaagc gggcactatc agccacgggc aaatgtgggc
    20701 aatcctgccg caaacctggc tgggtaacct ggtcggttcc gtcttcgttg ccatgctcta
    20761 tagctggggt ggcggtagcc tgctgccggt agataccagc atcgttcact ccgtcgcgct
    20821 ggctaaaacc actgcaccgg caatggtact gttcttcaaa ggtgcattgt gtaactggct
    20881 ggtttgcctg gctatctgga tggcgctgcg cactgaaggg gcggcgaaat ttatcgctat
    20941 ctggtggtgt ctgctggcat ttatcgcgtc cggctacgag cactccatcg ctaacatgac
    21001 gctgttcgcc ctctcctggt ttggcaacca cagcgaagcc tacacgctgg cgggtattgg
    21061 tcataacctg ctgtgggtga cgctgggtaa tactttatca ggtgccgtat tcatgggatt
    21121 gggttattgg tatgctacgc cgaaagcgaa tcgtccggtt gcggacaaat ttaatcaaac
    21181 tgaaacagct gccggttaat tactaagggg tttttacgtg gatcatttgc ctatattttg
    21241 ccaattacgc gatcgcgact gtctgattgt cggcggtggt gatgtcgcgg aacgcaaagc
    21301 aaggttgctg ttagacgcag gcgctcgctt aacggtgaat gcattagcgt ttattccaca
    21361 gttcaccgca tgggcagatg caggcatgtt aaccctcgtc gaagggccat ttgatgaaag
    21421 ccttctcgac acctgctggc tggcgattgc agcgacggat gatgacgcgc ttaaccagcg
    21481 cgtcagtgaa gccgctgaag ctcgtcgcat cttctgtaac gtggtcgatg cgccgaaagc
    21541 cgccagcttt attatgccgt cgattattga ccgctcaccg ctcatggtcg cggtctcctc
    21601 tggcggcacc tctccggttc tggcacgcct gttgcgcgaa aaactcgaat cactgctgcc
    21661 gttgcatctg ggccaggtgg cgaaatacgc cgggcaatta cgcgggcgag tgaaacaaca
    21721 gttcgccaca atgggtgaac gtcgccgttt ctgggaaaaa ctgttcgtta atgaccgcct
    21781 ggcgcagtcg ctggcaaaca acgatcagaa agccattact gaaaccaccg aacagttaat
    21841 caacgaaccg ctcgaccatc gcggtgaagt ggtgctggtt ggtgcaggtc cgggcgatgc
    21901 cgggctgctg acactgaaag gtctgcaaca aattcagcag gcagatgtgg tggtctacga
    21961 ccgtctggtt tctgacgata ttatgaatct ggtacgccgc gatgctgatc gcgttttcgt
    22021 cggcaaacgc gcgggatacc actgcgtacc gcaggaagag ataaaccaga tcctgctacg
    22081 ggaagcgcaa aaaggcaaac gcgtggtgcg gctgaaaggc ggcgatccgt ttatttttgg
    22141 ccgtggtggc gaagagctgg aaacactgtg caacgcgggt attccgttct cggtggttcc
    22201 gggtattacc gcagcttctg gttgctctgc ctattcgggt attccgctca cgcatcgcga
    22261 ttatgcccag agcgtacgct taattaccgg acacttaaaa accggtggcg aactggactg
    22321 ggaaaacctg gcggcagaaa aacaaacgct ggtgttctat atggggctga atcaggctgc
    22381 gactattcag caaaagctga ttgaacacgg tatgcctggc gaaatgccgg tggcaattgt
    22441 cgaaaacgga acagcagtca cgcagcgcgt gattgacggt acgctcacac agctgggcga
    22501 actggcgcag caaatgaaca gtccatcgct aattattatt ggtcgggttg ttggcctgcg
    22561 cgataaactg aactggttct ccaaccatta atgtaacccg ggccagagaa ttctggcctt
    22621 cttaatgact ctttttatat attcagcaaa taaaacataa ccccttaata aatattatct
    22681 gctaatgggt tatattcacc gttacagcga cttacaaaat taagccattc actcactgac
    22741 gctaattaaa tattcctgta tcgttcttaa tgagcagcct gcttatattc atataatcaa
    22801 tgaatattaa ttaatatata atacataggg aatgtaaatg aacaaattta ttaaagttgc
    22861 actggtaggc gcagtactgg ctacgttaac tgcatgtact ggtcatattg aaaaccgtga
    22921 taagaactgc tcttacgact acctgctgca cccggcaatt tctatttcta aaatcattgg
    22981 cggttgcggt cctactgcac agtaagtctc tgacagaaat cggctaacac cggtt
//
LOCUS       DAPOZO010000079        22948 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPOZO010000079 DAPOZO010000000
VERSION     DAPOZO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.079"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(3..1253)
                     /locus_tag="ROI21_003344"
     CDS             complement(3..1253)
                     /locus_tag="ROI21_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDX5112227.1"
                     /translation="MKSAFQYFLFLWIITASINNVNAENLTFMVGDDAGVGNLNGPSG
                     DKLISFSYPVGGVNNIVFYNPTSTTPTNAKIKLYWSELDTSSSRGFVYCTSKNNSSGM
                     PITVRHNMVDSGKMADGHRLYETNVTGLYYTLMLNRFWSYELRSSSPDIYVGDDGWHT
                     VQFSITGDYIRGQCNTSRYQAVGGLLHDATVEFYTDSSFNPAPNTNVQLKHTGDYLYS
                     FANEGPGVPNVSASKMLKIDFNLTDINIQLPTCFTSILLGESVTRSTVNLGEYTVGQI
                     TSDSATPVNFQISLQNCVRISSIKTKLKSNVVGVNNPLLLGNTLTGTDDAKGAGVLIE
                     GLPNSKNSQNFTLKPNDETSSYNDAEDDPGENNGIYNPDYPNANGRTTTQDLKFQATL
                     KRDGNATVKPGGFKATSTFQVTYE"
     gene            complement(1305..1904)
                     /locus_tag="ROI21_003345"
     CDS             complement(1305..1904)
                     /locus_tag="ROI21_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5112228.1"
                     /translation="MQMKSLHYGIFASITLSLLLFSTFSIAITDNIGLNVMTTVENRT
                     CQSEISNQGLVNLGTVGVGYFASNVSAEDNYPGGKTFTVTVKDCPSAGTTSSQLHIEF
                     KPVSGVMATGSQQIFANNHVGGAQNVGIVIFSQNNQSSDTVNVLSPTGQFRTVWPVAS
                     ANMNNSSWQFYTRMQKIDPLLPVGAGFVTSNVLVDIYYQ"
     gene            complement(1918..2478)
                     /locus_tag="ROI21_003346"
     CDS             complement(1918..2478)
                     /locus_tag="ROI21_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5112229.1"
                     /translation="MIKNTRYKIVLLAGIFLLPGALAQDINVDFVATIKGTTCNITLE
                     GTRVTADGNNQYTLRILNVALDKIINKMPEAQADFKLVANCDGNIGKITTSLAGNPSV
                     ELPYLISPLTSDNSSTTEYIAMGIKLRNASDTEFIPPDGSKKISWGEGLTKPELEMTV
                     ALRETRPGSGRAGNFKALATFNFTYE"
     gene            complement(2497..5097)
                     /locus_tag="ROI21_003347"
     CDS             complement(2497..5097)
                     /locus_tag="ROI21_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HDX5112230.1"
                     /translation="MKTTTSTKRHNLTRIAMYCSIMYSGATIGAESVEYDPTFLMGGN
                     ASSIDVSRYSDGNPTLPGVYDVSIYVNEQPVANLEIPFIAIPDKKNAQACITLKNLLQ
                     LHIKTPPADEENTILLPRDETLGNCLDLSLAIPKSSVNYDPSEQRLDINVPQAWVMKN
                     YQNYVDPSLWENGINAATLSYNINAYRSENSNYTNDSVYTSFNGGVNLGAWRLRSSGN
                     YSWRNDAGSNVEFMNRYVQRDITAIRSQLIMGESYTTGETFDSVSIRGIRLYSDSRML
                     PPVLANFAPTIRGVANTNAKVSITQSGYKIYETTVPPGPFVIDDLSPSGYGSDLIVTI
                     EEADGSKRTFSQPFSSVIQMLRPGVSRWDISGGQINKDDLHHEPNLLQATYYRGLSNL
                     FTGYTGFQVTDNHYAAGLLGIGMNTSVGAISFDVTHSSVDIPDDKRYQGQSYRISWNK
                     FFDLTDTSLNIAAYRYSTQDYLGLNDALTLIDEVEHPTQDLDPKTMRNYGRMKNQFTV
                     SINQPLRFGKDDYGSFYTSGSWSDYWGGSKSRSNYSVGYSNSASWGSYSISAQRSWDE
                     YSQTENSIYLSFSIPIEKLMGTEHRDSGFQSIDTQLSTNMDGNNQFNMSSSGYSNDNR
                     VSYSVNAGYGMNKTGKDLSNIGGYASYESPWGTLAGSVSATSDNNRQYSVNTDGGFVL
                     HSGGLTFSNDSFSDNDTIALVKAPGAKGARINYGNNTVDRWGYGVTSALSPYQENKIA
                     LDTENLENDIEMKSTSTVAVPRQGSVIFAGFETNQGQSAIMNIKRSDGKGIPFAADIH
                     DETNAIIGNVGQGGQAFVRGIQQQGTIKITWLEASMPKTCIAQYQQPNVTSEKIQQTI
                     ILNNILCQLQ"
     gene            complement(5132..5866)
                     /locus_tag="ROI21_003348"
     CDS             complement(5132..5866)
                     /locus_tag="ROI21_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDX5112231.1"
                     /translation="MLLNKKNIVALCVASSMAFSMPVMADIVISGTRVIYKSDQKSVS
                     VRLENKGNSHLLVQSWLDTGDDNAEPGSINVPFTATPPVSRIDAKRGQTIKLMYTGST
                     VLPSDKESVFWFNVLEVPPKPDASKVENQSLLQLAFRTRIKLFYRPAGLNGNPSEAPL
                     ALKWKWAEGKSGLSVYNPTPYYVSFNNAELIAGGKSNPLNVKMVAPKSEEIFTISGSG
                     GKATSGKVHFSAINDFGGAIEGDVNL"
     gene            complement(5956..6528)
                     /locus_tag="ROI21_003349"
     CDS             complement(5956..6528)
                     /locus_tag="ROI21_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5112232.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQINISGLVSDNTCETK
                     VDGGKQDGLVLLQTATVAEITAGVSETAVGAKAKPFSITVDCSKATPGGQPTLTFGSV
                     FFGNSRGTLNNDMSITTPSSGVNIALHNIDGTTVKQVKINNASDAYTKALTNNVAVFD
                     FKASYVREDDSTPATAGYVRTNTAYTITYN"
     gene            complement(6877..7356)
                     /gene="folK"
                     /locus_tag="ROI21_003350"
     CDS             complement(6877..7356)
                     /gene="folK"
                     /locus_tag="ROI21_003350"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDX5112233.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGTALGNLLKINKFHELNKW"
     gene            complement(7353..8771)
                     /gene="pcnB"
                     /locus_tag="ROI21_003351"
     CDS             complement(7353..8771)
                     /gene="pcnB"
                     /locus_tag="ROI21_003351"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDX5112234.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNISDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRSRRPRKRAPRREGTA"
     gene            complement(8810..9736)
                     /gene="gluQRS"
                     /locus_tag="ROI21_003352"
     CDS             complement(8810..9736)
                     /gene="gluQRS"
                     /locus_tag="ROI21_003352"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDX5112235.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(9773..10228)
                     /gene="dksA"
                     /locus_tag="ROI21_003353"
     CDS             complement(9773..10228)
                     /gene="dksA"
                     /locus_tag="ROI21_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDX5112236.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(10406..11110)
                     /gene="sfsA"
                     /locus_tag="ROI21_003354"
     CDS             complement(10406..11110)
                     /gene="sfsA"
                     /locus_tag="ROI21_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDX5112237.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(11125..11655)
                     /gene="thpR"
                     /locus_tag="ROI21_003355"
     CDS             complement(11125..11655)
                     /gene="thpR"
                     /locus_tag="ROI21_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDX5112238.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            11729..14158
                     /gene="hrpB"
                     /locus_tag="ROI21_003356"
     CDS             11729..14158
                     /gene="hrpB"
                     /locus_tag="ROI21_003356"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDX5112239.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVIISEGRSFPVERRYLPLPTHQRFDEAVAVATAEILRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPAVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMVVRAKSDDEAATAAKIAAILEEPPRVG
                     NCDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            14252..16783
                     /gene="mrcB"
                     /locus_tag="ROI21_003357"
     CDS             14252..16783
                     /gene="mrcB"
                     /locus_tag="ROI21_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDX5112240.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDYDDYDDYEDEEP
                     MPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLP
                     AAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDF
                     PDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRS
                     GFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLF
                     LSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYF
                     GRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDM
                     LSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAE
                     KAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIG
                     SLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRCYSESGRVMLVD
                     ALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAF
                     QTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTG
                     RQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMS
                     IYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEM
                     QQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            17003..19246
                     /gene="fhuA"
                     /locus_tag="ROI21_003358"
     CDS             17003..19246
                     /gene="fhuA"
                     /locus_tag="ROI21_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDX5112241.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYELARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            19297..20094
                     /gene="fhuC"
                     /locus_tag="ROI21_003359"
     CDS             19297..20094
                     /gene="fhuC"
                     /locus_tag="ROI21_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDX5112242.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            20094..20984
                     /gene="fhuD"
                     /locus_tag="ROI21_003360"
     CDS             20094..20984
                     /gene="fhuD"
                     /locus_tag="ROI21_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDX5112243.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            20981..>22948
                     /gene="fhuB"
                     /locus_tag="ROI21_003361"
     CDS             20981..>22948
                     /gene="fhuB"
                     /locus_tag="ROI21_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDX5112244.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISVPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLR"
BASE COUNT         5330 a         5570 c         6141 g         5907 t
ORIGIN      
        1 tgttactcgt aggtgacctg gaatgtactg gtagccttaa atccgccggg tttcacagtt
       61 gcattaccat cgcgttttaa tgttgcctga aatttcaggt cctgggttgt agtacgaccg
      121 ttggcattag gataatctgg gttataaatt ccattgtttt ctccaggatc atcttcagca
      181 tcgttatagc ttgatgtctc atcatttggc ttaagcgtga aattttgtga atttttgctg
      241 ttgggtaacc cttcaataag tactccggca ccttttgcat catcggttcc tgtcaaagta
      301 ttaccaagca acagaggatt attcactcct actacattag attttagttt tgttttaata
      361 ctgcttatac gaacacaatt ttgcagcgag atctgaaaat taacaggtgt tgcagaatct
      421 gaggtgatct gtccaacagt atattccccg agatttacgg ttgatcgcgt tacgctctca
      481 cccaaaagga tagacgtaaa gcatgttggc agttggatat tgatgtctgt taaattaaaa
      541 tcaattttta gcatcttaga tgcacttaca tttggaaccc ctggcccttc atttgcgaat
      601 gaatacaaat aatcaccagt atgttttaat tgcacgttag tatttggagc agggttaaaa
      661 ctgctgtcgg tatagaattc caccgtggca tcgtgcaaaa gacctccgac tgcttgatac
      721 ctggaagtat tacattgacc tcgtatataa tcacccgtaa tagaaaattg tacagtgtgc
      781 cagccatcgt ctccaacgta tatatctgga gagcttgagc gaagttcata cgaccagaat
      841 ctattgagca ttaacgtata atatagaccg gttacattag tttcatataa tctgtgccca
      901 tctgccattt ttcctgagtc aaccatgtta tgccggacag taattggcat accgctggaa
      961 ttattttttg aagtacaata tacaaaccca cggctacttg atgtgtccag ttcactccaa
     1021 tataatttta tttttgcatt ggtcggtgtg gtggatgttg gattataaaa tacaatattg
     1081 tttactccac caaccggata ggaaaatgat attagtttat cgcctgatgg gccattaagg
     1141 tttcctacgc cagcatcgtc accgaccata aaagtaagat tttcagcatt tacattatta
     1201 atgctggctg tgataatcca taaaaaaaga aagtattgga acgctgactt catcttatca
     1261 ctcctttgaa aaaataatat ttgtagatgc gttatgtctg gcatttattg ataataaata
     1321 tcaactaata cattgctggt gacaaatcct gcaccaacgg gaagtaacgg atcaattttt
     1381 tgcattcgtg tataaaattg ccacgatgaa ttattcatgt ttgcagaggc aactggccag
     1441 actgtgcgaa actgccctgt tggtgacagc acattcactg tgtccgatga ctgattattt
     1501 tggctaaaaa tcacgattcc tacattttgt gcaccgccga catgattatt ggcgaatatc
     1561 tgttggctac cagtggccat tacaccactg acaggtttaa attctatatg caactggctt
     1621 gatgttgtgc cagcggaggg gcaatctttt actgtcacag taaaggtctt tccgcctgga
     1681 taattatctt ccgcactaac attactggca aaatatccca ctccaacagt gccaaggtta
     1741 accaacccct ggttgctgat ttcgctttga caagttctgt tttcaacagt ggtcataacg
     1801 tttaggccaa tgttatccgt gatggctata gaaaacgtgc tgaataataa taagctcaga
     1861 gtaatgcttg caaatatccc ataatgtaat gatttcatct gcattattat tatcctttta
     1921 ttcgtaggtg aaattgaatg tcgcaagtgc tttaaaatta cctgctcttc ctgaacctgg
     1981 tcgggtttcg cgaagtgcta ccgtcatctc aagttccggt tttgttaatc cctctcccca
     2041 ggaaatcttt ttagaaccat ctgggggaat aaattcagtg tcagaggcat ttcgaagttt
     2101 aatccccata gcaatatatt ctgtggtgga tgaattatct gaagttaatg gagaaattaa
     2161 atagggtaac tctactgaag gattccctgc cagcgatgtc gtaatcttgc ctatgttacc
     2221 atcacaatta gcgaccagtt taaagtcggc ttgtgcttct ggcatcttgt ttatgatttt
     2281 atccagagca acatttaata tccgtagcgt gtactgatta ttgccatcag cggtgacgcg
     2341 tgtcccttcc agagtgatat tacatgtcgt gcctttgatt gtggcaacaa aatcaacatt
     2401 aatatcttgt gccagtgcgc cgggcagtaa aaaaatgcct gccaggagta ctattttata
     2461 ccgtgtgttt tttatcattg tcatctctct ttcgtattat tggagctggc aaagaatatt
     2521 attgagaata atcgtttgct ggattttctc cgatgtcaca ttgggttgct ggtactgcgc
     2581 aatacaggtt ttgggcatgc tggcttccag ccaggtgatt tttatcgtgc cctgctgctg
     2641 aataccgcga acaaatgcct gcccgccctg cccaacgttg ccaatgattg catttgtttc
     2701 atcatgaata tcggcggcga aggggatacc ttttccgtca ctgcgtttga tattcataat
     2761 cgcagattgt ccctgattgg tttcaaaacc agcaaagata accgagccct ggcgtggtac
     2821 ggcaaccgta ctggtgctct tcatttcgat gtcgttttcc aggttttcag tgtccagagc
     2881 aattttgttt tcctggtatg gtgaaagagc actggttacg ccatatcccc aacggtcaac
     2941 ggtattgttg ccgtagttaa tacgcgcccc ttttgcaccc ggtgctttaa ccagagcaat
     3001 ggtgtcatta tcactgaaac tgtcgttgct gaacgtcagt cctccgctgt gcaacacaaa
     3061 tccaccatct gtattaacgg agtactggcg gttattatcg ctggtagcag agactgaccc
     3121 agctagcgtt ccccatggcg attcatagct ggcataaccc ccaatgttgc tgagatcttt
     3181 gccggtttta ttcatcccat aacccgcgtt cacgctatag ctcacgcggt tatcgttgct
     3241 ataaccgcta ctgctcatat tgaactgatt attaccatcc atattggtac taagttgtgt
     3301 atcaatactt tggaaaccgg aatcacggtg ctccgtaccc atcagctttt cgataggtat
     3361 cgaaaagctg aggtaaatac tgttttctgt ttggctgtac tcatcccagg atcgttgagc
     3421 gctgatactg tagcttcccc atgaagcgct gttactgtag ccgaccgaat agttgctacg
     3481 gctcttactg cccccccagt aatcagacca gcttccagat gtatagaacg agccataatc
     3541 atccttccca aagcgtagcg gttggttaat gcttacggtg aactggtttt tcatgcgccc
     3601 gtagttgcgc atagttttgg gatcaagatc ctgagtggga tgttcaactt catcaataag
     3661 tgttaatgca tcattcaggc ccagatagtc ctgcgtggag tagcggtaag ccgcgatgtt
     3721 taatgatgta tctgtcaggt caaaaaattt gttccaggag atgcgatagc tttgcccctg
     3781 gtacctttta tcatccggta tatcgacact ggagtgggtg acatcgaagg aaattgcgcc
     3841 gacagaagta ttcatcccaa tgccaagtaa gcctgcagca taatgattat ctgtgacctg
     3901 aaaaccagta tagccagtga aaaggttact taaaccacgg taataggtcg cttgtagtaa
     3961 gtttggttca tgatgcaagt cgtctttatt aatttgcccg ccgctgatat cccagcgaga
     4021 aacacccggg cgcagcattt gaattactga agaaaatggc tgagaaaatg tacgcttgct
     4081 gccatcagct tcttcaatgg ttacgataag atcgctgcca taccctgacg ggctgagatc
     4141 atcaatgaca aaaggtcccg gtggaacagt ggtttcataa attttgtatc cactctgggt
     4201 gatgctgact ttggcgttag tatttgctac accacgaatt gtcggggcga agtttgccaa
     4261 tacaggcggc agcattcggc tgtcgctgta taagcgaatg ccgcggatac taacggagtc
     4321 gaacgtttca cctgtggtgt aggattcccc cataatgagc tgcgagcgaa tagcggtaat
     4381 gtcgcgctgg acgtagcgat tcataaactc aacattgctt cccgcgtcgt ttctccagct
     4441 atagttaccg ctgctacgca gacgccaggc ccctaaattg acgccaccat tgaacgatgt
     4501 ataaacgcta tcatttgtat aatttgaatt ttcactgcga taagcgttga tgttataaga
     4561 cagcgtagcc gcgttaatgc cattttccca aagtgatggg tccacatagt tttgataatt
     4621 cttcatcacc catgcctggg gaacattgat atccagacgt tgttcagagg ggtcatagtt
     4681 cactgaggat ttaggtatgg ctaacgacag gtcaagacag ttgccaagcg tttcatcccg
     4741 tggaagaagg atagtatttt cctcgtctgc cgggggtgtc ttgatatgca gttgtagtag
     4801 atttttcagg gtaatacagg cttgagcatt ctttttatcg gggatcgcga taaaggggat
     4861 ctccaggtta gcaacaggtt gctcgttaac atagatgcta acatcgtaaa caccaggcaa
     4921 agtcgggttg ccatcgctat agcggctaac atcaatactg gaagcgttgc cacccattaa
     4981 aaaggtaggg tcatactcaa cactttcggc tccgatggta gcgccgctat acatgattga
     5041 gcaatacatt gcgatacgcg taagattatg gcgtttagtc gatgtcgttg ttttcattac
     5101 attggcttcc tgctgcataa ggtccctagt cttacaaatt gacatcgcct tcaattgcgc
     5161 cgccaaaatc attaatcgcg gagaaatgta cctttcctga cgttgctttc ccgcctgaac
     5221 cgcttatggt gaaaatctct tcgcttttcg gtgcaaccat ttttacattg agcgggttcg
     5281 acttaccgcc agcgattaac tctgcgttat tgaaagagac gtaataaggc gttgggttat
     5341 atacactcag gccagacttc ccttcagccc atttccattt cagggctaat ggtgcttcag
     5401 acgggttacc gtttaatcct gccggacgat agaacagctt gatacgggta cggaaagcca
     5461 gttgtaacag gctctgattc tctacttttg aagcatctgg ttttgggggc acttccagca
     5521 cgttaaacca gaatacgctt tctttgtcgc tggggagtac ggtgctgccg gtatacatca
     5581 atttgatggt ttgaccgcgt ttagcatcaa tacgtgaaac gggaggtgtt gccgtaaatg
     5641 gtacattaat gcttcctggc tcagcgttat cgtcaccggt atccagccag ctttgtacta
     5701 acaggtggct attgccttta ttctccaggc gtacgctaac gcttttttga tcacttttat
     5761 aaatgacgcg tgtaccggaa ataacaatat cagccataac aggcatacta aatgccatac
     5821 tactggcaac acataatgcg acaatattct ttttatttaa caaaatatac catccatgac
     5881 atgaattgtt ttcaagacaa gggggcgaca ataggtgtcg cacccttgta gtaatttaaa
     5941 tcaccaacga ataacttaat tgtaggtgat ggtgtaagct gtgttggttc ttacataacc
     6001 agccgttgct ggtgttgagt cgtcttcacg aacataggag gctttgaagt caaagactgc
     6061 aacattattg gttaatgctt tagtgtaagc atcagatgcg ttattaattt tgacttgttt
     6121 aacagtggtg ccgtcaatgt tgtgcagggc gatattcaca ccagatgacg gggtggtgat
     6181 ggacatatcg ttattcaaag tgccacgact gttaccaaag aaaactgaac cgaatgtcag
     6241 agtcggttgg cctcccggag tcgctttgct gcagtctaca gtaatgctaa acggtttcgc
     6301 tttggcaccg acagcagttt cagatacacc agcggtaatt tcagcaaccg ttgcagtttg
     6361 cagaagaacc aggccatctt gcttgccgcc atcaactttt gtttcacagg tgttatcgct
     6421 aaccaagcca ctgatgttaa tctgaccacc gtccatatca gcagaagcag tacctgcaac
     6481 cattgccagc ataagaccag acaaggcaaa acctaatttt ttagacataa taatccttga
     6541 attatattcc atgagtttaa ctacaatcgg gtgctttcag acaaagaatc ccgatagaga
     6601 aaaatagatt gttttgttaa tgatgaatgt gaagatttac tgaatgtaac atctgttcat
     6661 caccaagtgg gatattgtgc atatttaaat ttatatatcc acttaattca tctttaagta
     6721 aaacttaatg tacaaagcag gaaaaatatt gtatgtgagg cggaaggtgt taacggggta
     6781 tataatatta attatggttg tagcttaggg aatatattaa ttgcaatggg gtaaggctat
     6841 ttttcggaat ttatttatat tcatgaaatc attaaatcac catttgttta attcatgaaa
     6901 tttatttatt tttaataaat ttcctaatgc tgttccatca ggaaacacca gctccggcgc
     6961 aatttcaaac agcggccaca gcataaatcc acgattcttc atatcgtagt gcggaacggt
     7021 caggcgttcg gtatttatta cttcattacc aaatagcatg atgtcgagat ccagtgtgcg
     7081 cggcccccag cgttccgctt tacggacgcg accttgttgt aactcaatac gttgtgtgtg
     7141 gttgagtaac tcttcaggtg caagagaggt ttccagcgcc acggctgcgt ttaagtaatc
     7201 gggttgatct tgcggcccca gcggtggggt gcggtaaaac gaagaaacgg caagaatgtg
     7261 gctttcaggg atatcgccta atgctttcag ggcagcattg acctgctcca gcggagaggc
     7321 cagattgctg cctatggcaa tatacgccac tgtcatgcgg taccctcacg acgcggtgcg
     7381 cgtttgcgtg gacgacgaga acggcgacgc ggtgacggct cttcatccag ctcgttgagc
     7441 atcccttttt gatctggtgg cgcggaaacc tggaactccc cccaccattt cacaagacgc
     7501 tgcagttcag cgttacgttc aacttcagct cgcaaggcca acaggtcata agccgcacgg
     7561 aacttaggat gctccagcag tttccaggcg cgtttaccct gacgtcggga catacgcaac
     7621 tgcaactgcc agatatcgcg ggttaacgtc gtcagacgtt tcgggatcgc cagtgaacgg
     7681 caggcttcgt ccagcacgtc gttcatcgcc agcgcgaaag cgtcgtgata ggtcaggccg
     7741 ctttcctggg cgatcttctg tgccgtctcc agcagtgggt accagaacat ggcggcaaac
     7801 aggaacgccg ggttcacgcg catatcgtta tggatacgcg tatcggtatt cttcagcacc
     7861 tgttcaatga tccgctccat cgggctgtcg ccattttccg tgaagtagcg ggtaatggtc
     7921 gggaacagcg gctggaacag atgatattca cacagcagct tataggtttc gtaaccgtag
     7981 cccgcttgta gcagtttaag cgattcttca aacaggcgtg ccggtgggat atcgttcagc
     8041 agggtagcga ggcgagggat cggttctgcg gtttccgggc tgatgcgcat acccaatttg
     8101 gcggcaaaac gtaccgcacg cagcatccgt accggatctt cacggtagcg cgtttccggg
     8161 ttaccaatca gacggataac gccgtctttc agatccttca tgccgccaac gtaatcacgg
     8221 acggtaaaat ctgccacgct gtaatacagg ctgttgatag tgaaatcgcg gcgctgggcg
     8281 tcttcttcga tggagccgaa aatgttgtcg cgcagcaaca tgccgttttg cccgcgttgg
     8341 gaggtcgtgc ggtcgctgat gttaccttcg tggtgtccac ggaaggtcgc aacttcgata
     8401 atctccgggc caaacatcac atgagccaga cggaaacggc gacccaccag gcggcagtta
     8461 cggaacagtt tgcgcacctg ctcaggcgtg gcgttagtgg ttacgtcaaa atctttcggc
     8521 tttttgccaa gtaacaggtc gcgcacgccg ccgccaacca gccaggcttc gtatcccgct
     8581 ttattgagcc tgtacattac cttcagggca ttttcactga tatctttgcg ggaaatagca
     8641 tgctgctcac gcgggatcac cgtcacctgt ggacgggcga ctgcctgttc agcctcgctt
     8701 tcctcgcggc ttagcacctt gcggcaaaaa ttagcgactc gggtaaaaat agtacacctc
     8761 ggtagtgtca aacatcattc aggacaaaaa aatagcggct aatcatagct cagcatgacg
     8821 catttgagaa tgttgaattt acaattgccg actcaggcac ggcggtgagc cgccagtttt
     8881 tgacggctga ctgaaggatt tgctcgacgc tgaaatcctg ccagtgtgtt tctacctgct
     8941 gacccagaaa atgaagtgcc gcgattaaca ccgggcgtgg atcgcctttc ggcaacgcag
     9001 gcgcatgatt ctgcttggaa agtttagcgc cttgtggatt aagcgccagc ggtaaatgga
     9061 tgtaatctgg cactttccag ccaaaaagct ggtacagcga gatttgccgc actgtcggtt
     9121 cgatcaaatc tgccccgcgg acaatttcgc tcacgccctg gaagtgatca tccaccacca
     9181 ccgccaggtt ataggcaaac aaaccatcac ggcgatgaat aataaaatct tcccgtgcca
     9241 gtttttcgtc ggcgtgaata atgccgcgca gcaggtcagt aaactgggtg accggatgct
     9301 gctggcggat acgtactgcg gcgttgtctg gtccatgatg taattcccgg caatgaccgt
     9361 cgtaaatacc gccaatgctt tgaatacgcg cacgcgtgca ggtgcagtaa taacttaatc
     9421 cttgctcatg taaccaggcg agtgcttcgc gataggcgtg gtgacgttgc gattgccaga
     9481 gaacgtcgcc gtcccagtgc agaccgtaat gttccagctg gcgcaggata gtctctgcgg
     9541 caccgggaac ttcacgaggc gggtcgatat cttcaatgcg taccagccag cgaccttgcc
     9601 tagcgcgagc ctgtaaatag ctgccaagcg cggcaatcag agagccaaaa tgaagctcgc
     9661 cggaaggaga gggggcaaag cggccaatat agtgtgtgtc tgtcatctct ttgaacaaaa
     9721 aataaggcgg gagcatttcc cgcctgtggt aaacgtgatg gaacggctgt aattagccag
     9781 ccatctgttt ttcgcgaatt tcagccagcg ttttgcagtc gatgcacaga tcggctgtcg
     9841 ggcgcgcttc cagacggcga ataccaattt caacaccgca ggattcgcag tagccgaaat
     9901 cttcgtcttc cacttttttc agcgttttct cgatcttttt gatcagctta cgctcgcgat
     9961 cgcggttacg cagttcgagg ctgaactctt cttcctgggc tgcacggtct accgggtccg
    10021 ggaagttggc tgcttcatcc tgcatatgtg taacggtgcg atcgacttca tccctgagtt
    10081 gattacgcca tgcttccaga atacgacgga agtgcgccag ctgggcttca ttcatatact
    10141 cttcgcccgg cttctcctga tatggttcca ccccagcgat ggcgagaata ctcagggacg
    10201 atgttttacg gttttgccct tcttgcatgt tgcttctcct taacacgcac tatcgatccc
    10261 catgttcggg ggaaaaatga ggccgctata aatagcagat gcttttccgg atagcaatta
    10321 tctaaacgta acacttgaca actgtgtgag gaaaagcgta tttgcgcacg cgaccagaat
    10381 gtaaattaac cagttactta ctctactaca atgtaaccgg tagtgatttt ttaagagcca
    10441 tgccttcggc agaaagttcc gctttgtaag ccagaatttc tacccccctc tgttgagctt
    10501 ctgacaatag ttgcgcgtat ttctcatcga tgtggcgcgc gggtgaaaac cgtgtaatgg
    10561 ctgaatgcag cacggcaaaa aagataaccg cacgctggcc ttcagccgct acgctcatca
    10621 actcccgaag gtgtttctga cctcgttcag tgaccgcatc gggaaaatat ccctgttcgt
    10681 tttccgctaa cgtaaccgat ttcacttcaa tatagcagtc tggacgcgaa tccgcctgca
    10741 acataaagtc aatacggctg cgttctgcgc cgtatttaac ttcgcttttc agcgagctat
    10801 agcctgacag ttctgaaatt gattcattaa ggatagcctc tttcgtcaac ctgttagccc
    10861 aaagcgtatt gacgcaaata attgcgccgc tctggctttg agtcaattcc caggtgtgtg
    10921 ggtatttccg tttggtgttg tctgaagtcg aataccagac ggtatcgcca ggcgttgcac
    10981 aaccggtcat cgcacccgta ttcgggcagt gtagcgttaa ttcgcgacca tcgggtgtga
    11041 tcacatcggc taaaaaacgt ttgtaacgct gaattagcgt cgcgcgctgt agagggggag
    11101 aaaattccat tacgacaatc cttgttattg cgttagcgcc cagcgtttta gcggcgtgta
    11161 gcgtgtgcgt ccacgggcaa acgaggaggc gtaaagggtg aactccgtca ccgtatacga
    11221 ccagttaaaa cctggcggcg ggattgtcac cgcctcgctg gcgtcgcgca ataaggtaat
    11281 atgtggatga aacggacgat tgctttgaaa acaaccgctg cgggcagcct gtgaacggag
    11341 catattcgcc agctggatta agccgcgtgg tggttgacgc atccctaacc acaccacacg
    11401 cgaacgcagc cattgaccgg cgtcatcaag cgtgagtgtg aaacccggtt gacgaatccg
    11461 cccggctaaa agagaaagcg ccttctcttt ctcggcgctc acttcgccta aaaatgccag
    11521 agtcagatgc aaattatcgg cggcgaccgg acgtcccgcg tcaggtggga agtgtgtggc
    11581 gcgccagtgg ataatctgtt cgcggatttc tgcaggtaag tcgatagcaa agaacagccg
    11641 ttgcggttca gacatgtgag gcactcggtt atgaactacc gcgatgctac aatgtggctc
    11701 gaagaatgtt aaccctctgg agcgttttgt gtcgtcgttg cccgttgctg ccgtcttacc
    11761 tgaattactt accgctctcg attgtgcgcc gcaggtatta ttaagtgcgc cgactggggc
    11821 cgggaaatca acctggctgc cgctgcaact gctggcgcat cccggcatta acgggaaaat
    11881 tatccttctg gagccgcgtc gtctggcggc gcgtaacgtt gcgcaacgac tggcggagct
    11941 gcttaacgaa aagccaggcg ataccgttgg ctaccggatg cgtgcgcaaa actgcgtcgg
    12001 gccgaatacc cgcctggaag tggttaccga aggcgtgctg acgcgcatga tccaacgtga
    12061 cccggaactg agcggtgttg gattggtgat cctcgatgag tttcatgagc gcagcttgca
    12121 ggcggatttg gcgttggcgc tgttactcga tgtgcaacaa ggtctgcgtg atgaccttaa
    12181 actgctgatt atgtcggcta cgctggacaa cgaccgcttg cagcaaatgc tgccagaagc
    12241 gcccgtcatc atctcagaag ggcgctcgtt tccggttgaa cgccgttatt taccactgcc
    12301 cacgcatcag cgttttgacg aagccgttgc ggtagccacc gccgaaatac tgcgtcagga
    12361 aagtggatca ttactgttat ttttacctgg cgtcggagaa attcagcgcg tgcaggaaca
    12421 actggcttcg cgcatcggca gtgatgtgtt gctctgcccg ctgtatggcg cgttgtcgct
    12481 gaacgatcag cgaaaagcga tccttccggc accgcaaggg atgcgcaaag tggtgctggc
    12541 gaccaatatt gctgaaacaa gtttaaccat cgaaggtatt cgtctggtgg tggattgtgc
    12601 ccaggagcgt gtggcgcgtt ttgatccgcg cacggggctt acgcggttga ttactcaacg
    12661 cgttagtcag gcatccatga cgcagcgtgc cgggcgcgcc gggcgtctgg agccgggtat
    12721 ctgcctgcat ttaatcgcca aagaacaagc agaacgcgct gccgcgcaaa gtgaaccgga
    12781 gatcttacaa agcgatcttt ccggtttgct gatggaatta ctgcaatggg gatgcagcga
    12841 tccggcgcag atgagctggc tggatcaacc gccagcagtg aatttactgg ccgcgaaacg
    12901 cctgttacgg atgctggggg cgctggacgg tgaacggctt agtgcgcagg ggcaaaaaat
    12961 ggcggcgctg ggtaacgatc cgcgtttagc agcaatggtg gtgagagcga agagcgacga
    13021 cgaagctgct accgctgcaa aaattgccgc tattctcgaa gagccgccac gggtgggcaa
    13081 ttgtgacctg ggcgtggcgt tttcgcgcaa tcaacccgcc tggcagcaac gtagtcagca
    13141 actgttaaaa cgtttaaacg tacgcggcgg tgaggcagac agttctctta tcgcgccgct
    13201 acttgccggg gcgtatgccg atcgcattgc tcgtcgccgt gggcaagatg gacgctatca
    13261 actggcaaac ggcatggggg cgatgctcga tgccgacgat gcgctaagcc gccacgaatg
    13321 gttgatcgca ccgttattat tgcagggcag cgcctcgccg gatgcgcgga ttttactggc
    13381 gctgccggtc gatattgatg agttagtaca acgctgcccg cagctggtac agcagtctga
    13441 cactgtggag tgggatgacg cgcaaggtac gctgaaagcc tggcgtcggc tgcaaatcgg
    13501 tcagttgacg gtgaaagtgc agccactggc gaaaccctcg gaagacgagt tgcatcaggc
    13561 gatgcttaac ggcattcgtg ataaaggttt aagcgtgctc aactggacgg cggaagcgga
    13621 acagctacgc ttgcgtttgt tatgcgccgc aaagtggttg ccggaatatg actggccagc
    13681 ggttgatgat gaaagtttat tggcggcgct ggaaacgtgg ctgctgccac atatgactgg
    13741 cgtacattca ctacgcggcc tgaaatcact cgacatttat caggcactac gcggattact
    13801 tgattgggga atgcagcaac gtctggatag tgaattgcct gcgcattaca ctgtgccgac
    13861 gggaagccgg atcgccattc gttatcatga agataatccg cccgcgctgg cggtgagaat
    13921 gcaggagatg tttggtgagg ccaccaatcc gacgatcgct caggggcgcg tgccgctggt
    13981 gctggagttg ctttcacccg cccaaaggcc gctgcaaatc acgcgtgatt tgggggcctt
    14041 ctggaaaggg gcgtatcgtg aggtgcaaaa ggagatgaaa gggcgttatc ccaaacatgt
    14101 ctggccggac gacccggcaa atactgcacc gacgcgacgg acgaaaaagt attcgtgaag
    14161 accaaagcaa atttgagaga tatcttcttc tgtcttgcaa cagaagaaca gaaaatcggg
    14221 cttttgcgcc tgaatattgc ggagaaaaag catggccggg aatgaccgcg agccaattgg
    14281 acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc gttacgaaga
    14341 tgacgattac gacgattatg atgactatga ggatgaagaa ccgatgccgc gcaaaggtaa
    14401 gggcaaaggc aaagggcgta agcctcgtgg caaacgcggc tggctatggc tgctgctaaa
    14461 actggctatc gtttttgccg tgctgatcgc catttacggc gtttatctcg atcaaaaaat
    14521 tcgcagccgt attgatggca aggtctggca attgcccgcg gcagtttatg gccgaatggt
    14581 caaccttgag ccagacatga ccatcagcaa gaacgagatg gtgaagctgc tggaggcaac
    14641 acagtatcgt caggtgtcga aaatgacccg tcccggcgaa tttaccgtgc aggccaacag
    14701 cattgagatg attcgccgtc cgtttgattt cccggacagt aaagaagggc aggtgcgtgc
    14761 gcgtctgacc tttgatggcg atcatctggc gacgatcgtc aatatggaga acaaccgtca
    14821 gttcggtttc ttccgtcttg atccgcgtct gatcaccatg atctcctcgc caaacggtga
    14881 gcagcgtttg tttgtgccgc gcagtggttt ccctgatttg ctggtggata ctttgctggc
    14941 gacagaagac cgccatttct acgagcatga tgggatcagt ctctactcca tcggacgtgc
    15001 ggtgctggca aacctgaccg ccggacgcac ggtgcagggt gcgagtacgc tgacgcaaca
    15061 gctggtgaaa aacctgttcc tctctagcga gcgctcttac tggcgtaaag cgaacgaagc
    15121 ttacatggcg ctgatcatgg acgcgcgtta cagcaaagac cgtattcttg agctgtatat
    15181 gaacgaggtg tatctcggtc agagcggcga caacgaaatc cgcggcttcc cgctggcgag
    15241 cttgtattac tttggtcgtc cggtagaaga attgagcctc gatcagcagg cgctgttggt
    15301 gggtatggtg aaaggggcgt ccatctacaa cccgtggcgt aacccgaaac tggcgctgga
    15361 gcgacgtaac ctggtgctac gtttgctgca acagcaacag attattgatc aagaactcta
    15421 tgacatgttg agtgcacgtc cgctgggtgt tcagccgcgc ggtggggtga tctctcctca
    15481 gccagccttt atgcaactgg tgcgtcagga gctgcaggca aaactgggcg ataaggtaaa
    15541 agatctctcc ggcgtgaaga tcttcactac ctttgactcg gtggcccagg acgcggcaga
    15601 aaaagccgcc gtggaaggca ttccggcact gaagaaacag cgtaagttga gcgatcttga
    15661 aactgcgatt gtggtcgtcg accgctttag tggtgaagtt cgtgcgatgg tcggtggttc
    15721 tgagccgcag tttgcgggct acaaccgtgc gatgcaggcg cgtcgttcga ttggttctct
    15781 tgcaaaacca gcgacttatc tgacggcctt aagccagccg aaaatctatc gcctgaacac
    15841 gtggattgcg gatgcgccaa tcgcgctacg tcagccgaat ggccaggtct ggtcaccgca
    15901 gaacgatgac cgttgttata gcgaaagcgg cagagtgatg ctggtggatg cgttgacccg
    15961 ttcgatgaac gtgccgacgg tgaatctggg gatggcgctg gggctgcctg cggttacgga
    16021 gacctggatt aaactgggcg taccgaaaga tcagctgcat ccggttccgg caatgctgct
    16081 gggggcgttg aacttaacgc caatcgaagt ggcgcaggca ttccagacca tcgccagcgg
    16141 tggtaaccgt gcaccgcttt ctgcgctgcg ttcagtcatc gcagaagatg gcaaggtgct
    16201 gtatcagagc ttcccgcagg cagaacgcgc ggttccggcg caggcggcat atctgacact
    16261 atggaccatg cagcaggtgg tacaacgtgg tacggggcgt cagcttgggg cgaaataccc
    16321 gaacctgcat ctggcaggga aaacagggac taccaacaat aacgtagata cctggtttgc
    16381 gggcattgac ggcagcacgg tgaccatcac ctgggtcggc cgtgataaca accagccgac
    16441 caaactgtat ggagccagcg gggcaatgtc gatttatcag cgttatctgg ctaaccagac
    16501 gccaacgccg ctaaatcttg ttccgccaga agatattgca gatatgggcg tggactacga
    16561 cggcaacttt gtttgcagcg gtggtatgcg tgtcttgccg gtctggacca gcgatccgca
    16621 atcgctgtgc cagcagagtg agatgcagca gcagccgtca ggcaatccgt ttgatcagtc
    16681 ttctcagccg cagcaacagc cgcaacagca acctgctcag caagagcaga aagacagcga
    16741 cggtgtagcc ggttggatca aggatatgtt tggtagtaat taacgtctaa gcgtgacata
    16801 ccggatggcg agcagccatc cggtaaaata acatcccacc caaagtatta accctttctt
    16861 ttcatctggt tgtttattaa cccttcagga acgctcagat tgcgtaccgc ttgcgaaccc
    16921 gccagcgttt cgaatattat cttatcttta taataatcat tctcgtttac gttatcattc
    16981 actttacatc agagatatac caatggcgcg ttccaaaact gctcagccaa aacactcact
    17041 gcgtaaaatc gcagttgtag tagccacagc ggttagcggc atgtctgttt atgcacaggc
    17101 agcggttgaa ccgaaagaag acactatcac cgttaccgct gcacctgcgc cgcaagaaag
    17161 cgcatggggg ccggctgcaa ctattgcggc gcgacagtca gctaccggca ctaaaaccga
    17221 tacgccgatt caaaaagtgc cacagtctat ttctgttgtg accgccgaag agatggcgct
    17281 gcatcagccg aagtcggtaa aagaagcgct tagctacacg ccgggtgtct ctgttggtac
    17341 gcgtggcgca tccaacacct atgaccacct gatcattcgc ggctttgcgg cagaaggcca
    17401 aagccagaat aactatctga atggcctgaa gttgcagggc aacttctata acgatgcggt
    17461 cattgacccg tatatgctgg aacgcgctga aattatgcgt ggcccggttt ccgtgcttta
    17521 cggtaaaagc agccctggcg gtctgttgaa tatggtcagc aagcgtccga ctactgaacc
    17581 gctgaaagaa gttcagttta aagccggtac tgacagcctg ttccagactg gttttgactt
    17641 tagcgatgcg ctggatgatg acggtgttta ctcttatcgc ctgaccggtc ttgcgcgttc
    17701 tgccaatgcc cagcagaaag gctcagaaga gcagcgttat gctattgcac cggcgttcac
    17761 ctggcgtccg gatgataaaa ccaatttcac cttcctttct tacttccaga acgagccgga
    17821 aaccggttat tacggctggt tgccgaaaga gggaaccgtt gagccgttgc cgaacggtaa
    17881 gcgtctgccg acagatttta acgaaggggc gaagaacaac acctattctc gtaatgagaa
    17941 gatggtgggc tatagcttcg atcacgaatt taacgacacc tttactgtgc gtcagaacct
    18001 gcgctttgct gaaaacaaaa cctcgcaaaa cagcgtttat ggttacggcg tctgctccga
    18061 tccggcgaat gcttacagca aacagtgtgc ggcattagcg ccagcggata aaggccatta
    18121 tctggcacgt aaatacgtcg ttgatgatga gaagctgcaa aacttctccg ttgataccca
    18181 gttgcagagc aagtttgcca ctggcgatat cgaccacacc ctgctgaccg gtgtcgactt
    18241 tatgcgtatg cgtaatgaca tcaacgcctg gtttggttac gacgactctg tgccattgct
    18301 cgatctgtac aatccggtga ataccgattt cgatttcaat gcgaaagatc cggcaaactc
    18361 cggtccttac cgcattctga ataagcagaa acaaacgggc gtttatgttc aggatcaggc
    18421 gcagtgggat aaagtgctgg tcaccctggg cggtcgttac gactgggcag atcaagaatc
    18481 tcttaaccgc gtagcgggaa cgaccgataa acgtgatgac aaacagttta cctggcgtgg
    18541 tggtgttaac tacctgtttg ataatggtgt aacaccttac ttcagctata gcgaatcgtt
    18601 tgaaccttct tcgcaagttg ggaaggatgg taatattttt gcaccgtcta aaggtaagca
    18661 gtatgaagtc ggcgtgaaat atgtaccgga agatcgtcca attgtggtta ctggtgccgt
    18721 gtataatctc actaaaacca acaacctgat ggcggaccct gagggttcct tcttctcggt
    18781 tgaaggtggc gagatccgcg ctcgtggcgt agaaatcgaa gcgaaagcgg cgctatcggc
    18841 gagtgttaac gtagtcggtt cttatactta caccgatgcg gaatacacca ccgacactac
    18901 ctataaaggc aatacgcctg cacaggtgcc aaaacacatg gcttcgctgt gggctgacta
    18961 taccttcttt gacggtccgc tttcaggtct gacgctgggc accggtggtc gttatactgg
    19021 ctccagctat ggtgatccgg ctaactcctt taaagtggga agttatacgg tcgtggatgc
    19081 gttagtgcgt tatgagctgg cacgagtcgg catggctggc tccaacgtgg cgctgcatgt
    19141 caacaacctg ttcgatcgtg aatacgtcgc cagctgcttt aacacctatg gctgcttctg
    19201 gggcgcagaa cgtcaggtcg ttgcaaccgc aaccttccgc ttctaatttc tcttttgggg
    19261 cacggatttc cgtgcccgct tcacaagttg gctgttatgc aggaatacac gaatcattcc
    19321 gataccactt ttgcactgcg taatatctcc tttcgtgtgc cagggcgcac gcttttgcat
    19381 ccactgtcgt taacctttcc tgccgggaaa gtgactggtc tgattggtca caacggttct
    19441 ggtaaatcca ctctgctcaa aatgcttggc cgtcatcagc caccgtcaga aggcgaaatt
    19501 cttcttgatg cccagccgct ggaaagctgg agcagcaagg cgttcgcccg caaagtggct
    19561 tatttaccgc agcagcttcc tccggcagaa gggatgaccg tgcgtgaact ggtggcgatt
    19621 ggtcgttacc cgtggcatgg cgcactaggg cgctttggtg ctgcagatcg cgaaaaggtc
    19681 gaggaagcta tctcgctggt tggcttaaaa ccgctggcgc atcggttggt cgatagtctc
    19741 tctggcggcg aacgtcagcg ggcgtggatc gccatgctgg tggcgcagga tagccgttgt
    19801 ctattgctcg acgaaccgac ctcggcgctg gatatcgccc accaggttga tgtgctggcg
    19861 ctggtgcatc gtttaagtca ggagcgtggc ctgacggtca ttgccgtgct gcacgatatc
    19921 aatatggctg cgcgttactg tgattatctg gtcgccctgc gcggcggtga aatgattgct
    19981 cagggaacgc ctgcggaaat tatgcgcggc gaaaccctcg aaatgattta tggcatcccg
    20041 atgggtattt tgccgcatcc ggcgggtgct gcacctgtga gttttgttta ttgatgagcg
    20101 gcttacctct tatttcgcgc cgtcgactgt taacggcgat ggcgctttct ccgttgttat
    20161 ggcagatgaa taccgcccac gcggcggcta ttgatcccaa tcgtattgtg gcgctggagt
    20221 ggttgccggt ggaattactg ctggcgctcg gcatcgtgcc ttacggcgtg gcggacacca
    20281 tcaactatcg cctgtgggtc agcgagccgc cattgccgga ctcagtgatc gacgttggtt
    20341 tgcgtacaga acctaacctt gaactgctga ccgaaatgaa accgtcgttt atggtctggt
    20401 cggcaggata tggcccttca ccagaaatgc tggcacgtat tgcgccgggg cgtggattta
    20461 acttcagcga cggcaaacag ccgctggcga tggcgcgtaa atcactgacg gaaatggcag
    20521 atttacttaa cctgcaaagc gcagcagaaa cgcatttagc gcaatatgaa gactttatcc
    20581 gcagcatgaa accccgcttt gtgaagcgtg gtgcacgccc gttattgctg acgacgctta
    20641 tcgatccgcg ccatatgttg gtcttcggtc caaacagctt gttccaggaa attcttgatg
    20701 agtacggcat cccaaatgcc tggcaagggg aaaccaactt ctggggcagt accgccgtca
    20761 gtatcgatcg tctggcagcg tataaagacg ttgatgtgct ctgttttgat cacgacaaca
    20821 gcaaagacat ggatgcgcta atggcaaccc cgctgtggca ggccatgccg tttgtccgcg
    20881 ccggacgctt tcagcgcgtt ccggcggtct ggttctatgg tgcgacgctt tctgcaatgc
    20941 actttgtgcg cgttctggat aacgccatcg gaggtaaagc gtgagtaaac gaattgcgct
    21001 ttttcctgcg ttattgctgg cgctgttagt gattgtcgct acggcactga cctggatgaa
    21061 cttctcgcag gcgctgccgc gtagccagtg ggcgcaggcc gcctggtcgc cggatattga
    21121 cgtcatcgag cagatgattt ttcactacag cttgttgccg cgtctggcga tttcgctgct
    21181 ggtgggcgcg ggcctggggc tggtgggcgt gctgtttcag caagtgctgc gtaacccgct
    21241 ggcggaaccg acgacgctgg gcgttgctac aggcgcgcaa ctggggatta ccgtcaccac
    21301 gctctgggcg atcccaggtg ctatggcgag ccagtttgct gcactggcag gggcttgtgt
    21361 ggttggctta attgtctttg gcgtcgcgtg ggggaaacgg ctgtcgccgg taacgctgat
    21421 tctcgcgggg ctggtagtga gcctttattg tggggcaatc aatcagttac tggttatctt
    21481 ccatcacgac cagctacaaa gcatgttcct gtggagcacc ggaacgctga cgcaaaccga
    21541 ctggggcggc gttgagcgtt tatggccgca gttgctgggc ggcgtgatgc tgacgttact
    21601 gctacttcgc ccgttaaccc tgatggggct tgatgatggc gtggcgcgca atctcggtct
    21661 ggccttgtcg ctcgcgcgtc tggcggcgct gtcgctggcg atagtcatca gtgcgctgct
    21721 ggtgaatgcg gtggggatta tcggctttat cgggttgttc gcgccgttgc tggcaaaaat
    21781 gctgggtgcg cggcgtctgc taccaaggtt gatgctggcg tcgctgattg gtgcactgat
    21841 cctctggctt tccgatcaaa tcatcctctg gctgactcgc gtgtggatgg aagtttctac
    21901 cggttcggtc actgcgttga tcggtgcgcc gctgctactg tggctgctgc cgcgtttacg
    21961 cagcattagc gtgccggata tgaaggtcaa cgatcgtgtc gcgactgaac gccaacacgt
    22021 gctggcgttt gccctcgcgg gcggcgtgct gctgttgatg gctgtggtgg tggcgctgtc
    22081 gtttggtcgt gatgcgcacg gctggacgtg ggcgagcggg gcgttgctcg aggatttgat
    22141 gccctggcgc tggccgcgaa ttatggcggc gctgtttgcg ggcgtaatgc tggcggtggc
    22201 gggctgtatt attcagcggc tgaccggaaa cccgatggca agcccggaag tgctggggat
    22261 tagctccggc gcggcgtttg gcgtggtgtt gatgctgttt ctggtgccgg gtaatgcctt
    22321 tggctggctg ttacctgcag gcagtctcgg cgcggcggtg acgctgttga tcattatgat
    22381 cgccgccgga cgcggtggat tttccccaca ccgcatgtta ctggcgggga tggcgttaag
    22441 taccgcgttc accatgcttt tgatgatgtt gcaggcaagt ggtgacccgc gaatggcgca
    22501 agttctgacc tggatttccg gttcgaccta caacgcgacc gatgcgcagg tctggcgtac
    22561 cggaattgtg atggtgattt tgctggcgat taccccgctg tgccgccgct ggctgaccat
    22621 tttaccgctg ggaggtgata ccgcacgagc cgtggggatg gcgctgacgc cgacgcgaat
    22681 tgcgctgctg ctgttagcgg cttgcctgac agcgactgcg acaatgacca ttggaccgtt
    22741 gagttttgtt ggtttaatgg caccgcatat tgcgcggatg atgggctttc gacggacgat
    22801 gccacacatc gtaatttcgg cgctggtggg aggattactg ctggtgttcg ctgactggtg
    22861 tgggcggatg gtgctgtttc cattccagat cccagcgggg ctgctgtcga cctttatcgg
    22921 cgcgccatat tttatctatt tgttgaga
//
LOCUS       DAPOZO010000080        22303 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPOZO010000080 DAPOZO010000000
VERSION     DAPOZO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.080"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(87..794)
                     /locus_tag="ROI21_003362"
     CDS             complement(87..794)
                     /locus_tag="ROI21_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDX5112245.1"
                     /translation="MVIGPFINASAVLLGGVFGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTVVAKAHNLFHHSRKKPAHESF
                     IQNFVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1193..3328
                     /gene="speC"
                     /locus_tag="ROI21_003363"
     CDS             1193..3328
                     /gene="speC"
                     /locus_tag="ROI21_003363"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDX5112246.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITVADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFNPMMADISPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTSGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDANGMKRLYGYVLK"
     gene            complement(3377..4633)
                     /gene="nupG"
                     /locus_tag="ROI21_003364"
     CDS             complement(3377..4633)
                     /gene="nupG"
                     /locus_tag="ROI21_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDX5112247.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(4835..5914)
                     /gene="mltC"
                     /locus_tag="ROI21_003365"
     CDS             complement(4835..5914)
                     /gene="mltC"
                     /locus_tag="ROI21_003365"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDX5112248.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASHKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(5978..6253)
                     /locus_tag="ROI21_003366"
     CDS             complement(5978..6253)
                     /locus_tag="ROI21_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDX5112249.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="ROI21_003367"
     CDS             complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="ROI21_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDX5112250.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVAALHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPSSVGLA
                     APVERLLQQLRTGAPV"
     gene            7494..8213
                     /gene="trmB"
                     /locus_tag="ROI21_003368"
     CDS             7494..8213
                     /gene="trmB"
                     /locus_tag="ROI21_003368"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDX5112251.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8213..8539
                     /locus_tag="ROI21_003369"
     CDS             8213..8539
                     /locus_tag="ROI21_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDX5112252.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYMAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            8723..9442
                     /locus_tag="ROI21_003370"
     CDS             8723..9442
                     /locus_tag="ROI21_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDX5112253.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9618..10664
                     /gene="ansB"
                     /locus_tag="ROI21_003371"
     CDS             9618..10664
                     /gene="ansB"
                     /locus_tag="ROI21_003371"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDX5112254.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10781..11728
                     /locus_tag="ROI21_003372"
     CDS             10781..11728
                     /locus_tag="ROI21_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDX5112255.1"
                     /translation="MKKQWIVGTALLMLMTGNVRADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQNNSIPALKKDVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDDFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVTILMKIDDIN"
     gene            complement(11853..12989)
                     /gene="hemW"
                     /locus_tag="ROI21_003373"
     CDS             complement(11853..12989)
                     /gene="hemW"
                     /locus_tag="ROI21_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDX5112256.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLNGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(12982..13575)
                     /locus_tag="ROI21_003374"
     CDS             complement(12982..13575)
                     /locus_tag="ROI21_003374"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDX5112257.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(13583..13873)
                     /locus_tag="ROI21_003375"
     CDS             complement(13583..13873)
                     /locus_tag="ROI21_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDX5112258.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(13870..14436)
                     /gene="yggT"
                     /locus_tag="ROI21_003376"
     CDS             complement(13870..14436)
                     /gene="yggT"
                     /locus_tag="ROI21_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDX5112259.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(14454..15158)
                     /gene="yggS"
                     /locus_tag="ROI21_003377"
     CDS             complement(14454..15158)
                     /gene="yggS"
                     /locus_tag="ROI21_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDX5112260.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELSVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            15176..16156
                     /locus_tag="ROI21_003378"
     CDS             15176..16156
                     /locus_tag="ROI21_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDX5112261.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAGLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            complement(16330..16746)
                     /gene="ruvX"
                     /locus_tag="ROI21_003379"
     CDS             complement(16330..16746)
                     /gene="ruvX"
                     /locus_tag="ROI21_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDX5112262.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16746..17309)
                     /locus_tag="ROI21_003380"
     CDS             complement(16746..17309)
                     /locus_tag="ROI21_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDX5112263.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(17418..18368)
                     /gene="gshB"
                     /locus_tag="ROI21_003381"
     CDS             complement(17418..18368)
                     /gene="gshB"
                     /locus_tag="ROI21_003381"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDX5112264.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(18381..19112)
                     /gene="rsmE"
                     /locus_tag="ROI21_003382"
     CDS             complement(18381..19112)
                     /gene="rsmE"
                     /locus_tag="ROI21_003382"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDX5112265.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQLDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(19192..19899)
                     /gene="endA"
                     /locus_tag="ROI21_003383"
     CDS             complement(19192..19899)
                     /gene="endA"
                     /locus_tag="ROI21_003383"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDX5112266.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVLPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(19994..20491)
                     /locus_tag="ROI21_003384"
     CDS             complement(19994..20491)
                     /locus_tag="ROI21_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDX5112267.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(20568..21962)
                     /gene="galP"
                     /locus_tag="ROI21_003385"
     CDS             complement(20568..21962)
                     /gene="galP"
                     /locus_tag="ROI21_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDX5112268.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         5504 a         5590 c         5881 g         5328 t
ORIGIN      
        1 gattcgcctc gtctcgtcaa tcaaatttct tcaatttagt gcagactgca caatctatca
       61 tcactttgcc gctattgcac gcattgtcag gcaaaccagg cggtccaggc ggcggaaagc
      121 ggcattgcca gaaggagtgc tggaagcatg ttgaccaccg ggaacatttt aatgccacag
      181 atgcgtaatc cggttgccag cagcaataaa ccgcctacgg cgctgaagtc tgccatcatc
      241 gacggtgtgg tcagcggtaa tatcagcgcg gcagcccacg ccagcgttaa ctggatgatc
      301 agtaatggaa tgctaatcac cgacaccgca atacccagcg agcaggcgaa gatcatcgcc
      361 gtaaagaaat caagaaatga cttggcgatt aaaatacttg gatcgccggt catcccttcg
      421 ttcatcgccc cgaagattcc ggtgccgctg gcgcaaaaca ggacaataat cgcgacaaaa
      481 ttctgaataa aagattcatg cgctggcttc ttacgcgagt ggtgaaacag attatgcgct
      541 ttggcgacca ctgtattgac gcctttttcc agcagacaaa tttcgccgat taatgctccg
      601 agtaaggttg ctaaaaccat cgccggaagg ttggcgcatt tcaccaccag taaaatacca
      661 atccccagcg atgccagacc aaaaattgat gtcatggaga cgcggatacg ttccggtaag
      721 cgttggctaa ggagtgcgcc gaaaacgcca cccagtaaga ctgcacttgc gttgataaaa
      781 gggccgatga ccacaagagt tcctgttagc tgtattgttt gattttctta ttatgaactt
      841 ttggcgccat cgatggcttg tttgttcgca aagtcctggc ttgcacgctt tagcgaaagg
      901 tgccatgatt gcgcgaattt tctcctctct gtacggagtt tgcccgatgc acgccacctc
      961 cttacattct ctcgcttatc gccgtttcgc gcgaaactct tctcttttta tgctactgct
     1021 catgcggtga agttaacgta cgctcactgc aggacaacag taaaatcaga gcgtttctgc
     1081 ttttactgat gtctggtggt cggagctggt gaccagtttg acccatatct catggggcag
     1141 ggttttccac cttgtccggt attcttactt cccctaaacg ggttttgcgc ttatgaaatc
     1201 aatgaatatt gccgccagta gtgaactggt atcccgactt tctactcatc gccgcgtggt
     1261 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ttgcggatag
     1321 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     1381 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     1441 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     1501 ttatgacacg ctgacgcagt acgttgagat gggtaatagc acctttgctt gccctggaca
     1561 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     1621 cgagaacatc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     1681 tcatgaagga tcggcgaaag atgcgcagaa atttgcagcc aaagtctttc atgccgataa
     1741 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     1801 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     1861 gttgattcag gcgggggcga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     1921 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     1981 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctggcgatta ttcagctggg
     2041 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccgttg ggcatctgtg
     2101 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttaacc cgatgatggc
     2161 ggatatctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggatct ttgtgaccca
     2221 gtcggtgcac aaacagcagg cgggattctc acagacgtcg cagatccata aaaaagataa
     2281 ccatatccgt ggacaggcgc gtttttgccc gcataagcgg ttgaataatg cctttatgct
     2341 ccatgcttct accagcccgt tctatccgct gttcgccgcc ctggacgtta acgccaaaat
     2401 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     2461 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     2521 tggcaaattg tggcaggatt atccgacgtc agtgttagcc agcgaccgcc gttttttcag
     2581 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     2641 tgatccgtgc aagctgttac tcaccacgtc gggtatcgat gccgaaaccg gcgaatatag
     2701 cgattttggc gttccagcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     2761 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     2821 gctggcacaa ctggtggcga tgctggcgca gtttgaacag catattgagg atgactcgcc
     2881 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     2941 cctgcgtcag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     3001 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaatc cccaggatgc
     3061 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     3121 aattgcggca gaaggagcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     3181 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     3241 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcga atggcatgaa
     3301 acggttgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     3361 gtttttcttt gcgtaattag tggctaaccg tctgtgtacc tgtcgggaca cgaacgtgtt
     3421 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggagtaacct gcgaaaatca
     3481 gccatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaacc actttaccgc
     3541 tcacgatgcc gccgaggata cagccgaagc cgttggtcat catcaggaac atcccctgcg
     3601 cgctggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     3661 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     3721 gagtcgggtc gccgtaagca aacagagcaa aacgcaggat ccacgccaca atactgatca
     3781 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     3841 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     3901 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     3961 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     4021 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     4081 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     4141 caatatacag ctgcatgtgg cttaattcga agccggacag gctcaccacc cacattgcca
     4201 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     4261 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     4321 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     4381 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     4441 atttgtcggc cacaatcccc agcagcgcag gcataaagac cgcggcgata cccagtgaac
     4501 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     4561 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     4621 gcttaagatt catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca
     4681 tttgaccgtg cgagatgatg cgattctatc aacttcgcgg actggttttt tgttacctgt
     4741 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     4801 tacccctgag catcgtcagg ggcggttagt ggaattatcg gcggcggtag gatttttgcg
     4861 cggtattcac tttataaaga taacggcgag attccgcaga aggatggcgg gtcgtcagcg
     4921 tctgataaac atcgcccggc gtcatggtgt taataatatt ggctgcctga atcttgtcat
     4981 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     5041 gccgcgatgt tgggttatca attccgccga gataaacgtt gttcagcatc gccagatacg
     5101 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     5161 atttcccctg cgagcggaac acatctttcc cggcagtatg ttgtaccacc tgcattaatc
     5221 ccagcgcatc ggaacggctg actgcatacg ggttaaagga agattcagtc tgcataattg
     5281 ccagaatcag cgactcatca acgccatatt tgtgtgacgc ctggcggacc ataccgagat
     5341 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     5401 tacgcagccc gttgctgcgg ctcttcagac ggtttttcag cagataatcc gcgaagttgc
     5461 tcgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     5521 aaggttcttt cgaaatcgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     5581 cgtcccccat cagcaacgtt ttgattattg cccggcgcaa atgcgcggca ggttctgtcc
     5641 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     5701 attgatcggt gtatttcacg tagtccttag gaccggcgat cactacctct ttgaatcccc
     5761 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtgtctt
     5821 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     5881 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     5941 ttatgcaagt tgccggaggc gacctccggc actgttttta ttttttgtct tccggcgtat
     6001 agccctcgat atgcacctct ttaccctcga acaggaagtt gaccatctcc tgttccagca
     6061 gtttgcggtg ctcggcattc atcatgttga gtttcttttc attaatcagc atggtttgtt
     6121 tgtgctgcca ctgcgcccag gcttctttgg agatctcgtt atagatgcgt tttcccagtt
     6181 cgccggggta cagctgaaaa tcctgacctt ctgcttcacg ttgcaggaaa gtacaaaaaa
     6241 tcgttctgct cataaatcat cctctttatc gactcacgcg ctaaaccggc gcgccagtgc
     6301 gtaactgctg taacaaacgc tccacgggag ccgccaggcc aactgacgac ggttgcgcta
     6361 agttatacca gagcgcattg ccttcatcca tgcagccggt gaatgacgac acgggaagcc
     6421 acataggcac aatatctaag tgaaaatggc tgaaggtatg gcgaaacgcg gtcagttgcg
     6481 tcaggttatc ggcagcaatc tgccgttgcg ccagccactg ccgcaaactt tcttcgtcgg
     6541 caaactgtgg gaaacagtat aaaccgcccc acaatccact cggcgggcgc tgcgccagca
     6601 atacttcatc ttcgtgctgt aacagcagaa agtagccggt gcgctccggc agcgtctgtt
     6661 tcggtttttt gcccggataa agcgaccagc tattgttggc ggcggcaata catccgtttt
     6721 gtagcggaca gagcgaacat ttcggtttcg agcgcgtaca aatcatcgcg cccaaatcca
     6781 tcatcgcctg attaaaccgc tccacgccaa ccgcgggcgt cacctgctcg ctcaaactcc
     6841 atagtttatt ctcgacctct tttttcccgg gccagccgct tacagcatag cagcgcgcca
     6901 gcacgcgttt gacgttaccg tcgagaatcg gaaagtgctt acccagagaa agcgagagaa
     6961 tcgcgcctgc ggtggaacgc ccgacgcccg gcagtgctgc aacttcctca aaggtttccg
     7021 ggaatttacc gccgtgtagg gccgccactt gttgtgccgc tttatgtagg ttgcgcgcgc
     7081 gggcgtaata gccaagcccg gtccacaagt ggagaacttc gtcgagcggc gcattggcga
     7141 gatcggtcac cgttgggaag cgcgccataa agcgttcaaa ataggggata acggtcgcaa
     7201 cctgagtttg ttgcaacatc acttctgaga gccatacttt gtagggcgtc ttgtcaattt
     7261 gccagggcag cgttttccgc ccgtatttat cgtaccagtc cagaacctgg gctgaaaatt
     7321 gcgacgcttg catggtcacc gaattcactg ttgttggggg caagattgca gcacagcgac
     7381 ggcagggtgt aaaccggaac tttccgcagc cacggcctta tcatgcttgc atccggcaac
     7441 taactttgga taatgcccgt tttcagaaca ctttcacaag cgactaaatc tttatgaaaa
     7501 acgacgtcat ctcaccggaa tttgatgaaa acggccgccc gctgcgccgt atccgtagtt
     7561 ttgtgcgccg ccaggggcga ctgaccaaag gccaggaaca tgcgttggaa aactactggc
     7621 cggtgatggg cgttgagttc agtgaagata tgctggattt ccccgcgctt tttggccgtg
     7681 aagcgccggt gacgcttgag attggttttg gcatgggggc gtcgctggtg gcaatggcta
     7741 aagatcgccc tgagcaggac ttcctcggca ttgaagtgca ttcaccgggc gttggtgcgt
     7801 gcctggcttc tgcgcatgaa gagggcttaa gcaacctgcg cgtgatgtgt cacgatgcgg
     7861 ttgaagtgct gcataaaatg attcctgaca attcattgcg catggtgcag ctctttttcc
     7921 ctgacccgtg gcacaaagcg cgccataata aacgccgtat cgttcaggtg ccgtttgccg
     7981 aactggtaaa aagcaaactg caactggggg gcgtattcca tatggcgacc gactgggaac
     8041 cttatgcgga acatatgctt gaagtgatgt cttctattga cgggtataaa aacctgtcag
     8101 agagcaatga ttacgtaccg cgtccggcat cacgtccggt gacgaaattt gaacaacgtg
     8161 gtcatcgtct tggtcacgga gtatgggact taatgttcga gagggtgaaa taatggcaaa
     8221 gaaccgtagc cgtcgtctgc gtaaaaaaat gcacatcgac gaattccagg aattaggatt
     8281 ttcggtggca tggcgattcc cggaaggtac atcggaagaa cagattgata aaaccgttga
     8341 tgactttatt aacgaggtta tcgaaccgaa caaactggcc tttgacggca gcggttatat
     8401 ggcctgggaa ggtctgatct gcatgcagga aatcggcaaa tgcaccgaag aacatcaggc
     8461 gattgtgcgt aagtggctgg aagagcgcaa actgggcgag gtacgcacca gcgaactttt
     8521 cgacgtttgg tgggactaag aaagcatacg ggcgatgaca aatgcaaaac tgcctgatgt
     8581 gctacgctta tcaggcctat aaagatgcac gatcgagtag gctggataag gcgtttacgc
     8641 cgcatccggc atggaaaacg cgcactttgt tatcaatctg gggccagcaa atgctggcct
     8701 gatttgttct tgagggaaga ctatgatgcg caaaatgctg ctggcggcag cactttcagt
     8761 gacggcaatg accgctcacg ccgactacca gtgcagcgtc acgccgcgtg acgatgtgat
     8821 tgtcagcccg cagaccgtgc aggtgaaggg cgaaaacggc aatctggtga tcacgccaga
     8881 cggcaacgtg atgtataacg gtaagcaata ttccctgaat gccgcccagc gcgagcaggc
     8941 gaaggattat caggctgaac tccgtagcac cctgccgtgg attgatgaag gcgcgaaaag
     9001 ccgcgtcgaa aaagcccgta ttgcgctgga taaaattatc gttcaggaga tgggcgaaag
     9061 cagcaaaatg cgcagccgtc tgaccaaact tgatgcgcag ctgaaagagc agatgaaccg
     9121 cattatcgaa acgcgcagcg atggcctgac gttccactat aaagccattg atcaggttcg
     9181 cgccgaaggc cagcaattag tgaatcaggc aatgggcgga attttacagg acagcattaa
     9241 tgaaatgggc gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga acgtgctggg
     9301 aagcctgggc ggtctgcaat cctcaatcca aaccgagtgg aaaaagcagg aaaaagattt
     9361 ccagcagttt ggcaaagatg tttgtagccg cgttgtgact ctggaagata gccgcaaagc
     9421 cctggtcggg aatttaaaat aatcctctat tttaagacgg cataatactt ttttatgccg
     9481 tttaattctt cgttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt
     9541 tgtaaattgt ttaacgtcaa atttcccatg caaagctaag ggataatgcg tagcgttcac
     9601 gtaactggag gaatgaaatg gagtttttca aaaagacggc acttgccgca ctggttatgg
     9661 gttttagtgg tgcagcattg gcattaccca atatcaccat tttagcaacc ggcgggacca
     9721 ttgccggtgg tggtgactcc gcaaccaaat ctaactacac agcgggtaaa gttggcgtag
     9781 aaaatctggt taatgcggtg ccgcaactga aggacattgc gaacgttaaa ggcgagcagg
     9841 tagtgaatat tggctcccag gacatgaacg atgatgtctg gctgacactg gcgaaaaaaa
     9901 ttaacaccga ctgcgataaa actgacggct tcgtcattac ccacggtacc gacacgatgg
     9961 aagaaaccgc ttacttcctc gacctgacgg tgaaatgcga caaaccggtg gtgatggtcg
    10021 gtgcaatgcg cccgtccacg tctatgagcg cagacggtcc attcaacctg tataacgcgg
    10081 tagtgaccgc agctgataaa gcctccgcta atcgtggcgt actggtagtg atgaatgaca
    10141 ccgtgcttga tggccgtgat gtcaccaaaa ccaacaccac cgatgtagcg accttcaagt
    10201 ctgttaacta cggtcctctg ggttacattc acaacggtaa gattgactac caacgtaccc
    10261 cggcacgtaa gcacaccagc gacacgccgt tcgatgtctc taagctgaat gaactgccga
    10321 aagtcggcat tgtttataac tacgctaacg catccgatct tccggctaaa gcactggtag
    10381 atgcgggcta tgatggcatc gttagcgctg gcgtgggtaa cggcaacctg tataaaaccg
    10441 tatttgacac ccttgcaacc gctgcgaaaa acggcactgc agtagtgcgt tcttcccgcg
    10501 taccgacggg cgctaccact caggatgccg aagtggatga tgcgaaatac ggcttcgtcg
    10561 cctctggcac gttgaacccg caaaaagcgc gcgttctgct gcaactggct ctgacgcaaa
    10621 ctaaagatcc gcagcagatc cagcagatct tcaatcagta ctaatcactt cgccccggta
    10681 tcgtgccggg gcttattcac ttcagactca cgtccattgc caatttttat taccctaatg
    10741 ataatcacca gaataaatta ttccgcgcga gggttttcgg gtgaaaaagc aatggattgt
    10801 tggtacggcg ctgcttatgt tgatgactgg taatgtccgg gcagatggtg aaccgccaac
    10861 tgaaaatatc ttaaaagatc aattcaaaaa gcagtatcac ggcattctca agcttgatgt
    10921 catcacctta aaaaatcttg atgctaaggg taatcaggcc acctggtcag cggaaggcga
    10981 tgtctcttcc agtgacgatc tctatacctg ggtcggtcag ttggcagatt acgagctgct
    11041 cgaacagacc tggacgaaag ataaaccggt gaaattctcg gcgatgttaa ccagtaaagg
    11101 aacgcccgcg tctggctggt cggtgaactt ttactctttt caggcggcag ccagcgatcg
    11161 tgggcgggtg gttgacgata tcaaaaccaa taataaatat ctgatcgtga atagcgaaga
    11221 tttcaattat cgctttagtc agcttgagtc tgcattgaat aaccagaata actcgattcc
    11281 tgcactgaaa aaggatgtaa aagcgcttga taagcaaatg gtggcggcgc agaaagcagc
    11341 ggatgcatat tggggaaaag atgcaaacgg taagcaaatg acccgcgaag acgcctttaa
    11401 aaaaattcac caacagcgtg atgattttaa taaacagaac gatagtgagg cgttcgcggt
    11461 taaatatgac aaagaggttt atcaaccggc gattgcggca tgtcataaac agagtgaaga
    11521 gtgttatgaa gtgccaattc agcagaaacg agattttgat atcaacgaac aacggcgaca
    11581 gacttttctg caatcacaaa aacttagccg taaattgcag gatgactgga taacgctcga
    11641 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcacgat
    11701 tttgatgaaa attgacgata tcaactaggt ttatgaacgc tggaaaaaag ataccgaaca
    11761 attgcggcgc aatggggtga ttaagtaatt tttgcgatga cgcaagagtt gtagccggat
    11821 gttgcgtaca cgccgcatcc ggcaatacaa atttactcag ccagaaaaag ctccagcagc
    11881 gaatttaaaa acaacttccc atgttccgtt atctgccagt aatccgcaca ttctgtgaga
    11941 taaccctgag cgatagcctc gtctaactga gggcgaataa cctcttctga aaggccagta
    12001 tactggctaa actccacgcg cggcgcggct tccagcaaac ggaagcgatt cataaagaac
    12061 tcaaacggtt tatctgcagc ttcgacatca cgctggcttt ccagataccg cccctgcata
    12121 aaaccacgtg gatgacgcgt tttggtggta cgcagaatgc gcccatccgg gaaggtcact
    12181 ttgccatgcg cgccgcagcc aataccgatg tagtcaccaa agcgccagta gttgagattg
    12241 tgctggcact gataaccggg tttggcgtaa gcggaagttt catattgctg ataacccgct
    12301 gcggttaata actgatgccc ctgttcgaaa atgtcccaca gcgcgtcatc gtccggcagt
    12361 acaggagggc gcgaaccaaa cagcgtattg ggttcgatgg tcagttgata ccaggaaaga
    12421 tgcggcggat tcagttcaat ggcctggcgt agatcgccta gcgcctcttc cagtgattga
    12481 tccggtagcc catgcatcaa atcgaggtta aagctacgta accctaaacc gctcgccaga
    12541 tttgccgctc gtttcgcttc ttgcgggcca tgaatgcgcc caagtcgttt cagcttttct
    12601 tcgctaaaac tctgtacgcc aatggagatg cggttcacac cagcacgctg ataatcaaca
    12661 aagcgatcgg cttcaaccgt accagggttc gcttccatcg taatttctgc atccgctgcc
    12721 agcggcaaac gcgcacgtac gccgttcagc agcgtttgca tcgccgggcc ggaaagcagg
    12781 ctcggcgtac caccgccaat aaagattgtc tttacttcac ggccctgagc gtaagccaca
    12841 tcgttgtcca gatcgttaag cagatgctga acataatcgt cgtgcggcac ttctcctttc
    12901 aacgcgtgag agttgaaatc gcagtacggg catttctgca cgcaccacgg gatgtgaatg
    12961 tagagactca gcggcggtaa tttaaccatt acgtaaagcg tccagcagca gtttcaacgc
    13021 ctgaccacgg tgggaaatgg cgctcttttc ttcgcgggtc agttcggcag cagttttccc
    13081 ttcggaaggt acgaagaaga ttggatcata accaaagcca ccagtgcccg ctggttcacg
    13141 agtaatcacg cccggccagc tgccgtggca caccagcgga gtaggatctt ccgcgtgacg
    13201 cagatatacc agcacgcagt ggaaacgcgc ctgacgttgg tcgtccggta cgtctttcat
    13261 tgtttccagc agtttttgca gattcttttg atcggtcgcg tcttcaccgg aataacgcgc
    13321 ggagtaaata cccggcgcgc cacccagcgc atctaccgcc agaccggagt cgtcggcaat
    13381 tgccggtaaa ccggtcactt ttgccgcatg gcgcgctttc agaatcgcgt tttcgataaa
    13441 ggtcaggccg gtttcttcag cggaatctac gccgaggtct gtttgggcaa cgatatcaag
    13501 accgaagtcg ctaagcagcg acgccagctc acgcacttta ccggcattgc cggttgcgag
    13561 gacaactttt tgcataggat acctaattaa ttaacgccgc gatttctggc gggatttgtt
    13621 gcggattaat gattttaatt tgtttgtggc ggccaagttc gcctttttca atcaccacct
    13681 ggcttttggc aacccggaat tgcttaccga gaaacttcac cagatggctg ttggcctggc
    13741 cgtcaaccgg cggcgcggta atggcgactt ttacctcgtc gccatgtaaa ccgacaatag
    13801 aatcacggct ggctttcggc tgaatataga gccgtaaaac cagaccgtca tcattaactg
    13861 ttacggcact cataacgcca tccacagccc cggcagcagc atatttccgg tcgcctgcaa
    13921 gacttctgcg acacccatat tgatgacata cagcagcaga acgaggatca tcggcgagaa
    13981 atcaatccca cccattgccg gtagcaagcg gcgaatcggg cgcagcagcg gatcggccag
    14041 ctgaatcagc acgtattcaa tcgggctacg accctggctt acccagctca taatcgccat
    14101 caccagcagg acccagaaaa tcagcaggcc gatggttttc agcagaatca ataaaccggc
    14161 aatccaaatg attggcagga aggtcaccac tttaaacagc acgatggctt taatgaaact
    14221 gagaatatag gcaaccagca gcgaggcgct atcaatgggg cccattgccg gaataacgcg
    14281 gcgcagtggc ccgataatcg gctgcgttac cttcactaca aactgtgaga aggggttgta
    14341 aaaatcacaa tgagcccact gcatccagat gcgtaataac agcaccatgg tatacagctc
    14401 aatgaccgtt gaaagcagga aagtcaacgt attcatggcg ttcctttaat tccttatttt
    14461 ttagagtaat cacgcgcacc aaaaattgca gtgccgatac gaaccatcgt gctacctgcc
    14521 gcaatggcgg cttccatatc gtccgacatt cccagagaga gcgtgtcgat atgcgggtag
    14581 cgcgttttca gtccggcaaa tgctacagcc atttggcgtg caacttcaaa ctgccttaca
    14641 tattctgact caggcgcagg gattgccatc agcccgcgca gacgtaaacg cggtagttca
    14701 gcgaccgcag ctgccagctc gtccagttca gccagttgaa tcccggactt actgttttca
    14761 tcactaatgt taatttgaat cagtacgtta agagggggaa gttctgccgg gcgctggtcg
    14821 ttcagacggg tagcgatgcg caaacggtcg atggtatgac accagtcgaa atgctctgcc
    14881 accaggcggc ttttattaga ctgcaacggg ccaataaaat gccattctaa tcctgttacg
    14941 ctcagttcct gaaagtggcg aattttatct accccttcct gaacgtagtt ttcaccaaat
    15001 tgacgctgcc cggcatcaat ggcttctgcg atggcgctcg caggttttgt tttactgact
    15061 gcaagcagcg taatttcttc tggagaacgg ccgcaacgcg ttgcagccgc tgagattttg
    15121 tcccggacct gtgccaggtt atgcgcaata tcgttcattt tccgaggatc ttagtatgaa
    15181 tatggaagaa attgtggccc ttagtgtaaa gcataacgtc tcggatctac acctgtgcag
    15241 cgcctggccc gcacgatggc gtattcgcgg gagaatggaa gctgcgccgt ttgacgcgcc
    15301 ggacgtcgaa gagctactgc gggagtggct ggatgacgat cagcgggcaa tattgctgga
    15361 gaatggtcag ctggattttg ctgtgtcgct ggcggaaaac cagcgattgc gcggcagtgc
    15421 gttcgcacaa cggcaaggta tttctctggc gttacggctg ttaccttcgc actgcccgca
    15481 gctcgaacag cttggcgcac caccggtatt gccggaatta ctcaagagcg agaatggcct
    15541 gattctggtg acgggggcga cggggagcgg caaatctacc acgctggcgg cgatggttgg
    15601 ctatctcaat caacatgccg atgcgcatat tctgacgctg gaagatcctg tggaatatct
    15661 ctataccagt cagcgatgtt tgatccaaca gcgggagatt ggtttgcact gtatgacttt
    15721 cgcatcggga ttgcgggctg cattgcggga agatcctgat gtgattttgc tcggagagct
    15781 gcgtgatagc gagacaatcc gtctggcgct gacggcggca gaaaccgggc atctggtgct
    15841 ggcgacatta cacacgcgcg gcgcagcgca ggcagttgag cgactggtgg attcgtttcc
    15901 ggcgcaggaa aaagatcccg tgcgtaatca actggcaggt agtttacggg cggggttgtc
    15961 acaaaagctg gaagtggata aacaggaagg acgcgtggcg ctgtttgaat tactgattaa
    16021 cacacccgcg gtggggaatt tgattcgtga agggaaaacc caccagttac cgcatgttat
    16081 tcaaaccggg cagcaggtgg ggatgataac gtttcagcag agttatcagc agcgggtgaa
    16141 agaagggcgc ttgtgacgtt gctgtgtctg atacgaattg taaatatgcc cgatgcgcaa
    16201 gcttatcggg cctacaaggg aagcgtaact ttgaattcgc actcactgta ggttggataa
    16261 ggcgtttacg ccgcatccaa catgaaacaa cgagcacatt gtcagaaatc tgtgcgctag
    16321 taaatcgcct taatatccct gctcgaaata gctttcgaga ataataaccg cagaggcaga
    16381 gtcgactttg cctttgttga gcgcccgata gccgccctgt tcaaacagac cggaacgtgc
    16441 ctccacggtg ctaagacgct cgtcatggag ctttacttca acaccgaaac gaccatgaat
    16501 acggttggca aatttacgcg ctctggcggt taacggctgt tcggtgccgt ccatattcag
    16561 cggcaaaccg acgatgattt cgtccggctg ccactctttc agtaaacgct cgataatgtt
    16621 ccagtccggc gtaccgtcct gcgctttaat tgcaggcaaa gggcgagcgg tgccggtaat
    16681 gcgttggccg accgctacgc caatgctttt ggtgccgaag tcgaaggcga gtaaggttcc
    16741 actcatcagg cgtgtcctgc cacaccaggc atggtgagaa tatccacacc aatcagtttt
    16801 gccgcctcgc gccagcggtc cgcaatcggc gttttgaaca gaatattcag atctgccggg
    16861 gcggttagcc acgcgttatc gagaatttct tgttccagtt gacctttctc ccaggaggca
    16921 tagcccagag ccaccaatac gtcagacggt tgtttatcgg tgccgagcgt ttccagcaca
    16981 tcgcgggaag tggtcatcac cgtgttgtct gaaatgcgaa tgctggaagc aaaattggag
    17041 ggcggagtat gcaaaataaa cccgcgatct tcagccagtg ggccgccgag cataaccggt
    17101 ttatccagac ggattgattc atcacgcggc tccggcgtga tcttcagttt ttccagaatc
    17161 ccttcaattt tgagattttc cagcggcttg ttgacgatga tccccattgc accattggta
    17221 ttatgttcgc aaatgtagac cacggaacga cggaaaatcg gatcctggag agcaggcatg
    17281 gcaataagaa agtgatgctg taaattcatt gtcagaggtt ctgtttcctg gttcaaaaaa
    17341 gcaacagcgc ccagtatgcg tggaaagaag ggcgctgtca ctcagagtct caacgagatc
    17401 cttctcgcta aggtgggtta ctgctgctgt aaacgtgctt cgatggcatc cattaacatt
    17461 ccggtgatcg acaccggaaa ttctgcttca atctcacgaa tacaggttgg gctggtgacg
    17521 ttaatttcag tcagacggtc gccgatgata tccagaccaa caaaaatcag ccctttttct
    17581 ttcagcgtcg gcccaatctg acgggcgatt ttccagtcac tttccgtcag cggacgaggt
    17641 tcaccgcgac caccggcagc cagattgcca cgggtttcgc ccccctgcgg aatacgcgcc
    17701 aggcaatacg gcaccggctc gccatccact accagcacgc gtttgtcgcc atctttaatg
    17761 gctggcaggt aattttgcgc catgcagtag cgagtgccat gctcagtcag ggtttcggca
    17821 atcacgccga ggtttggatc gccttctttc acgcggaaaa tcgacgcgcc gcccataccg
    17881 tccagtggct taagaatgat gtcgctgtgt ttctcccaga acgcttttag ctgcgcttta
    17941 ttgcgcgtaa ccagcgtttc tggcgttaag tcagagaacc aggcagtaaa cagtttctcg
    18001 ttacagtcgc gcaggctctg cggcttgtta acgatcagcg tccctttctc ttcggcacgt
    18061 tccagaatat aggtcgcgta gataaactcg gtatcaaacg gcgggtcttt acgcatcagg
    18121 atcacatcga gatcggccag cggcagatcc tgttcaccga cgaacgaaaa ccactcttcg
    18181 tagttctgct tcacgttcag cgtgcgggta tgggcgcggg cttcgccatt gatcagatac
    18241 agatcgccca tctccatata gtgaagttcg taaccacgac gctgtgcttc cagcaacata
    18301 gcgaaactgg aatctttctt gatgttgatg tttgcgatgg ggtccatcac gatgccgagc
    18361 ttgatcatta ttcttctccg ttagcccaaa tcgccaaatc gtacttgtag cgcggtaatg
    18421 gcggtgagcg cagttgtctc tgtacgcaaa acgcgaggtc ccaacaggat atcagtaaat
    18481 tgatagcggg cagtcatggc aatttcatct gccgataaac cgccttccgg gccaatcagc
    18541 aggcggacgc gttcaaccgg taacggcaac gtgttgatgc tgttactggc gcgcgggtga
    18601 agattcagtt tcagtccttc atcctgctct gcacaccagg cttccagatc catcgctgga
    18661 cggatttccg gcacccggtt acgaccacac tgctcacagg cagcaattgc aatcttctgc
    18721 cactgctgga gcttcttgtt cagacgttca ctatccagtt taacgccgca gcgttcagaa
    18781 aaaagtggcg taatgaggct tacaccgagt tcgatcgatt tctggatagt aaattccatt
    18841 ttttcgccac gcgacatcac ctgaccgagg tgaatatgca gcggagattc acgatcgtcg
    18901 agttggcctt ccagcacttt cacttccacg ctttttttac tggcgctggt aatttcggcg
    18961 tcaaagacct ggttgctgcc gtcaaacaat tgcaacgcct gtcccggccc catgcgcagt
    19021 acgcgcccga tatggttggc ggcatcttcg caaagcgcga tgtgagaatg gctggttagt
    19081 ggttcaggat gataaatgcg ggggatacgc atagttaaaa atccgcgtcg tctccccacg
    19141 cggttgtacg cgtggggtag gggttaacaa aaagaatccc gctagtgtag gttagctctt
    19201 tcgcgcctgg caagcgcgtt gcacatacgg gttatgattg ccctgcacct tcgcgatgcg
    19261 ttcatcgcgc tcgcactccc agtcggtaac cggatacatc ttgttccatg cgttgaacag
    19321 ctgcgtttgc tggcgggaga gtgtcaggtt gtattggtcg cgcatataga agtaggtgcg
    19381 cgcaatggca ccgcgtgcac gcgctggtgg ttcggcagct ttttctttga aatcgacctt
    19441 catggcgcat tgaccgtact ggccttcgcc gccattccac tggctgtaca taaagttgcc
    19501 gcgatcgcca ttcacctcgc cgactgacgg ctgcaggtta tgcatatcgc tttccatctt
    19561 gcgatagacc ggatctttgg cgcaattttt acgtccaccg tcctgccagc actggcgctg
    19621 gtgaccgaat tgccaggcgg gaaggacgtg ttcccactct acgcggctgg cgcggttttc
    19681 atttttgcgc acctgatagc cgcacgattg cagatcaaca acgccttttt tgccctgcca
    19741 gttaatttta catccgcaat aaaacgtacc gggcgcgtca gcgtggactt ttaccgccgc
    19801 ggctttcgcc tgagaaaaac tattgatacc ttcggccaac gccgggccgg aaaatgctgc
    19861 gctcagtacc accgcagcaa tagacaaata acggtacatc acttactccg tgttaaaacg
    19921 agccagcaac gtagcgaaag ttgttttagt atgcaatcag ccagatcagg aaagttcctg
    19981 atagttcagg tggttacttt gcaaccagct gttcgccgca gtgaacacag cgatagacgg
    20041 cttcgccacg aactacgcga ttatgacggc gtacggtaag ctgatgctcc tggcacttgc
    20101 agcggtaggg gaaggtgttg cggcgcacgg attgcagttc gaactgatgc gtacggcggg
    20161 cgggaacgcc cagcacgctt tccatcatcc acttccactc tttgccatgt ggcgctacgc
    20221 ggccgaaatg tttccatacc agcaaatgtg ccagttcgtg cggcaccact tcttcaataa
    20281 aggcttcact gttttccaac agcaaaacgg gattgaggcg gatttcatag ctttccagcc
    20341 aggctgttcc ggcggaggtg ccgcgctggg tgtaagagag ttttggctcc gggtagttac
    20401 gccctagctt caggttggcc tgggcgagtt tttcccgcag gcgacgcata acggcctgtt
    20461 ggatggcgat agggagacgg gatgttttca tagcggcaga ggatagagcg aagaaaagat
    20521 gactgcaaga ggtggcttcc tccgcgacgg gaggaagcct gaggagatta atcgtgagcg
    20581 ccgatttcgc gcagtttacg acctttcatc agattacgtt caatatgttc cagcgaaacg
    20641 tgtttggttt ccggtaccag ccacaatgtc agcaggataa acagtacgtt cagagccgcg
    20701 tacacccaga aggtgttggc gttacccagc gtgttgagca tggtcaggaa cgttgcgcca
    20761 acgatcatgt tggcaatcca gttggtggcg gtggagcagg tgatgccaaa atcgcggcct
    20821 ttcagcggct gaatttcgga gcacagtacc caaatcagcg gaccggcact catggcaaaa
    20881 ccgacaataa acatcagcag catggcgatg gcgaaatact gcgccgacgg agagtggata
    20941 ccgatatgca tcattgtacc gagtacgccc ataccagcag ccatcaccag gaagcccagc
    21001 gttagcgttg gtttacgtcc ccagcggtca acaaggccga ttgcgataaa ggtggcaagt
    21061 acgttggtca ggccgacaat cactgtcccc cacatttgct cggtggtgtt ggtataaccc
    21121 gccagttcga agattttcgg cgcgtaatac atgatgacgt tcatcccggt gaattgctgc
    21181 attacctgta acagtacgcc aaggaacacc gcgcggcgga agttgctatt ctctttaaac
    21241 agcgcccagc cactctgttt aacctgcaaa ctttcacgga tttcatccag ttcgcgtttc
    21301 gcttccgcgc tggtgtcacg caggcgtagc agcacgcgtt cggcatcaac aaaacggcgt
    21361 ttggcggcaa accaacgtgg gctgtctggc aggaagaaga caccaatcag cagcaaaatt
    21421 gccgggatga taatcacacc cagcatccag cgccatgcac cggtgtagct gaaggcggta
    21481 tcagaaagat aagcaccgag gatcccgata gtgatcatca actgatacat agagatcata
    21541 ctgccgcgaa ttttttccgg cgcaatttca gagaggtaca gtggtgcggt ataagaggcc
    21601 acacccaccg ccagccccag cagaacgcgg gaaagaatca gtacttcaac gtttggcgca
    21661 gccgcagaga acagcgaacc ggcaacaaac aaaattgcgc cgatcatcag gctctttttg
    21721 cgcccgagtt taaaggagag ccagccgctg cccaccgcac cgactgccgc accgaacatc
    21781 atggagctta cgacccattc ttgcgtgtgc gaagtaatct ggaattcatc tgcaataaac
    21841 ggcagtgcgc cagcaattac accgatatcc aggccaaaga gtaatcccgc cagagcggca
    21901 aggaagcaga cgaaaaacgt cattgccttg tttgaccgcc cctgtttttt agcgtcaggc
    21961 atgatgccct ccaatatggt tattttttat tgtgaattaa gataggtgag tacgacgtaa
    22021 aaagatgtga agcaaatcac atcagtgtaa tcgcttacac tgacatttat cttagttatg
    22081 atttaagtgc atgaaaataa ttgagttata tcatcataga ttaaacgctg ttatctgcaa
    22141 ttaagacttt actgaaaaga aatgtaacaa ctgtgaaaac cgaataataa tatgaaaata
    22201 ttacaaatct ttgtctgttg tggttggtgt aatcggtttc agttgtgatg ataatctgcg
    22261 gtgaaacgtg atcaggaaga atgatgttat tgtgcgggcg gtt
//
LOCUS       DAPOZO010000081        21798 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPOZO010000081 DAPOZO010000000
VERSION     DAPOZO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.081"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            65..859
                     /gene="panB"
                     /locus_tag="ROI21_003386"
     CDS             65..859
                     /gene="panB"
                     /locus_tag="ROI21_003386"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDX5112269.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAA
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            871..1722
                     /gene="panC"
                     /locus_tag="ROI21_003387"
     CDS             871..1722
                     /gene="panC"
                     /locus_tag="ROI21_003387"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDX5112270.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLVRYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELV"
     gene            complement(1804..2694)
                     /locus_tag="ROI21_003388"
     CDS             complement(1804..2694)
                     /locus_tag="ROI21_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDX5112271.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGRQAVRQEVSQEFAQRLLSKGMSRE
                     DVAEMATLSLTEVDKLINSN"
     gene            2968..3348
                     /gene="panD"
                     /locus_tag="ROI21_003389"
     CDS             2968..3348
                     /gene="panD"
                     /locus_tag="ROI21_003389"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDX5112272.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3352..4581)
                     /locus_tag="ROI21_003390"
     CDS             complement(3352..4581)
                     /locus_tag="ROI21_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDX5112273.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYTTLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFSRSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(4645..5085)
                     /locus_tag="ROI21_003391"
     CDS             complement(4645..5085)
                     /locus_tag="ROI21_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDX5112274.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLA
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5190..5960)
                     /locus_tag="ROI21_003392"
     CDS             complement(5190..5960)
                     /locus_tag="ROI21_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5112275.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(5957..6883)
                     /locus_tag="ROI21_003393"
     CDS             complement(5957..6883)
                     /locus_tag="ROI21_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5112276.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            6992..7654
                     /gene="can"
                     /locus_tag="ROI21_003394"
     CDS             6992..7654
                     /gene="can"
                     /locus_tag="ROI21_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDX5112277.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(7695..8231)
                     /gene="hpt"
                     /locus_tag="ROI21_003395"
     CDS             complement(7695..8231)
                     /gene="hpt"
                     /locus_tag="ROI21_003395"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDX5112278.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8437..10821
                     /gene="gcd"
                     /locus_tag="ROI21_003396"
     CDS             8437..10821
                     /gene="gcd"
                     /locus_tag="ROI21_003396"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDX5112279.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAETA
                     SPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYEPT
                     SPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSDEH
                     TFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATTGK
                     LAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELVVP
                     APEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQMRY
                     EGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGNPM
                     EQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEVVW
                     KKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSNGE
                     KLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(10868..12418)
                     /gene="cueO"
                     /locus_tag="ROI21_003397"
     CDS             complement(10868..12418)
                     /gene="cueO"
                     /locus_tag="ROI21_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDX5112280.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNNPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12584..12931
                     /locus_tag="ROI21_003398"
     CDS             12584..12931
                     /locus_tag="ROI21_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDX5112281.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13037..13903
                     /gene="speE"
                     /locus_tag="ROI21_003399"
     CDS             13037..13903
                     /gene="speE"
                     /locus_tag="ROI21_003399"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDX5112282.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDASVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTNQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            13919..14713
                     /gene="speD"
                     /locus_tag="ROI21_003400"
     CDS             13919..14713
                     /gene="speD"
                     /locus_tag="ROI21_003400"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDX5112283.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(14751..15113)
                     /locus_tag="ROI21_003401"
     CDS             complement(14751..15113)
                     /locus_tag="ROI21_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDX5112284.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVED
                     AARTLKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15310..17907)
                     /gene="acnB"
                     /locus_tag="ROI21_003402"
     CDS             complement(15310..17907)
                     /gene="acnB"
                     /locus_tag="ROI21_003402"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDX5112285.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18262..20019
                     /locus_tag="ROI21_003403"
     CDS             18262..20019
                     /locus_tag="ROI21_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDX5112286.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QNAPDISAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPVTQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVREFGYSMGVATTYALFSSIDWDDEDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNHITGEHLTDNNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPALSRDSQRQAAASQFQQRTYTAPVITRYTQRQAAAQRFNEAEHYGSYDDFREFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRKEAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNRTARLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(20090..21514)
                     /gene="lpdA"
                     /locus_tag="ROI21_003404"
     CDS             complement(20090..21514)
                     /gene="lpdA"
                     /locus_tag="ROI21_003404"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDX5112287.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
BASE COUNT         5338 a         5931 c         5333 g         5196 t
ORIGIN      
        1 attgccccca ccaatgtgac tgataaaatg ccagacagca cgacattact tatcaggata
       61 cgttatgaaa ccaaccacta ttgcttcatt gcaaaaatgc aaacaagaaa aaaaacgttt
      121 cgcgaccatc accgcttacg actatagctt cgcaaaactg tttgccgatg aagggcttaa
      181 cgtcatgctg gttggcgatt cactgggcat gacggttcag gggcatgact ccactctgcc
      241 agttaccgtt gccgatatcg cctaccacac tgctgctgta cgtcgcggtg cgccaaactg
      301 cctgctgctg gctgatctgc cgtttatggc gtatgccaca ccagaacaag cctttgaaaa
      361 cgccgcaacg gttatgcgtg ctggggccaa catggtcaaa attgaaggcg gtgagtggct
      421 ggtagaaacc gtacaaatgc tgaccgaacg tgccgttcct gtttgtggtc acttaggttt
      481 aaccccacag tcagtgaata ttttcggtgg ctacaaagtc caggggcgcg gcgatgaagc
      541 ggccgatcga ctgctcagcg atgcattagc cttagaagct gctggggcac aactgctggt
      601 actggagtgc gtgccggttg aactggcaaa acgcattacc gaagcactgg cgatcccggt
      661 tatcggtatt ggcgcaggca acgttaccga tggacagatc ctcgtgatgc acgatgcgtt
      721 cggcattact ggcggtcata tccctaaatt tgccaaaaat ttcctcgccg aaacgggcga
      781 catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt atccgggcga
      841 agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct gccgctgctg
      901 cgtcagcaaa ttcgccgcct gcgtatggaa ggtaagcgcg tggcgctggt gccaaccatg
      961 gggaatctgc acgatggcca tatgaagctg gttgacgaag ccaaagcccg cgccgatgtg
     1021 gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga cctggtacgt
     1081 tatccacgga ccttgcagga agactgcgag aagctaaaca aacgtaaagt ggatttagtt
     1141 ttcgcccctt cggtaaaaga gatctacccg aacggtacag aaacccacac ctatgttgat
     1201 gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt tcgcggcgtt
     1261 tcgactattg tcagcaagct gttcaacctg gtccagccag atatcgcctg cttcggtgaa
     1321 aaagattttc agcaactggc gctgatccgc aaaatggttg ctgatatggg ctttgatatt
     1381 gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctgag ttcccgtaac
     1441 ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaggt tttaagttcg
     1501 attgccgaca aattgcaggc tggcgaacgg gatctcgatg aaattattac tattgccggg
     1561 caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgtga tgccgacaca
     1621 ttgctggaag tctctgaaaa cagcaaacgt gcagtgattc tggtagccgc ctggcttggc
     1681 gatgcccgcc tgatcgacaa caaaatcgtc gagctggtgt aatacttaac tggcgctacg
     1741 gctgatggcg ccagttattc attgccacgt tatctgtaag cgataattca taacgcagac
     1801 aacttagtta gagttaatta gcttatcaac ctctgtaaga gataaagttg ccatctctgc
     1861 aacgtcttcc cgagacattc ctttactcag aagacgctgg gcgaattcct gacttacctc
     1921 ctgtctaacc gcctgccttc ccttctcttc aaaccactgc gccagcgtca tcatagactt
     1981 ccctcccgtt tccctgtctc tcagcacgcc ataaaacaaa tccgcttgtt cagtatgacc
     2041 gcgttgcagc atatagtttt gcatggcaac taactgactt ccgctagtgt acccttcgtc
     2101 aatcagcgtg accagttgct ccagcaataa cattaagtcg cgctggcgaa tatgtttttg
     2161 cagtagttcg agaatcgcaa tccgccgatg ttgcatgatt tcgtcatccg gtgtgatggt
     2221 gatatccacc agcgggaaag ggttgttgta aacgcgtcgc gccagctccg gcgagtaaaa
     2281 catatcaaac cagcacattg agagcggata aggtgtggcc tcgccctgat aaaacagaat
     2341 cggcaccacc agcggcagct tatcgtgatc cgcctccaga tgccggtgca tggcggcaat
     2401 ggcatagcgc atcatgcgaa aggtcatttt cttatccggc ttgctttgat gttcaatcac
     2461 cacatgcaaa taaccgggat tgccctgcat ttgtacggaa tagagtacgt ccgtgctgtg
     2521 tcctttcagg ctttcctcaa taaaactccc cgactcaaga tgcagcgtgt cgaggtcaca
     2581 cagttcgcgt agctccgctg gcaaatgaat atccagaaag tcgcgagccg tttccgcatg
     2641 cattaaaaat tgtttaaata ccgcatcatg cggtgtggta ctcggtgcat ccatcttccc
     2701 tgccttccgt gttgtcttgc gtgaaggcag tatccagtca aggcgcgccg tcgtcagcca
     2761 cctctttagt gtttaccgag cagcgttcag agaaatatca attcgttaca ggcgttacat
     2821 ggcacgcttc ggcgtggtac gcaaactgct gatgcaaatg ccatcagatg agtagacact
     2881 catcaaaaat cgggcaatac tgcgtgagaa tttcctcaaa gcaggcatcg cctgcttcgt
     2941 taacgacagg gtagaaaggt agaagttatg attcgcacga tgctgcaggg caaactccac
     3001 cgcgtgaaag tgactcatgc ggacctgcac tatgaaggtt cttgcgccat tgaccaggat
     3061 tttcttgacg cagctggtat tctcgaaaac gaagccattg atatctggaa tgtgaccaac
     3121 ggcaagcgtt tctccaccta tgccatcgcg gcagaacgcg gttcgagaat tatttccgtt
     3181 aacggtgcgg cggcccactg cgccagcgtc ggcgatattg tcatcatcgc cagcttcgtt
     3241 actatgccag atgaagaagc tcgcacctgg cgacccaacg tcgcctattt tgaaggcgac
     3301 aatgaaatga aacgtaccgc gaaagccatt ccggtacagg ttgcctgatt gttatccctg
     3361 cggctggtta ctcaccagcc gcgacatcgt ctccagcgaa tccgttctta agatataaag
     3421 tcgttttagt aacaacggat tatcccccgg ttttactttg cctttcatgg ttgtcaccgc
     3481 caggtgaaat ccggcttctt ttgctgcctt cacggctttg tcattaaatc cgccaaacgg
     3541 atacgaaaga taccagacat gcggattaaa ttgcgccaga gcgcgtcgtg aacgtgaaaa
     3601 atcaaacaga atattatgct cactacggct cagtaatatg ggtcggcgat aaccatccac
     3661 ccgatgcaaa aaatgggtat gtgactggaa atcaaaaaca tcgcgaattt cgttaagctc
     3721 agaaacgctc ataaattgca gcgattttgg gtcccacttc tgcgggtgac gtttgatgcg
     3781 cgaggtaaca ataaacgccg tcgccttcat gccatattgt ttcaacacag gatacgcata
     3841 gcggctcacc gacttgaggc catcatcaaa ggtaatcacc accgctcgcg cagggagatt
     3901 gatcttattc ttcacgtaac cttccagctg caccatgctc agtgtcgtgt atcccctgtc
     3961 gcgcagccag gccatctggt tattgaaagc gcgtaccgag gtggtcgtcg aagtatggcg
     4021 aaaacgggtg ttttcttcgt cgcgcagaat atggtgatag gttagcaccg gcaggccatt
     4081 atcgggttgg gcatccagcg cgctgatata ggccagtcga tcgccaatac ggatctgata
     4141 ccaggtttga tttaacctgt ctttcagttt atgcaaaatc gggtagcgca aattgtccgc
     4201 cagtacccca aacggcgcac tgcccgcact cggcgcgtta tagaccggcg tatctttcca
     4261 ggtaactaag ttctgattac tcagcggctt gttgaggtca cccaaaccgt cttcaacttt
     4321 ttgtcgcccc tgaaccggct cgagatgacc tttatcgata aaccctttac caaagccaaa
     4381 attaaatgcg taataacttg cggcagtggg ctccacggca atgatttgtc cggcccgaat
     4441 attccccacc gtcaccatct tgtcgccaat ttgcgcccag accgcagcat tttcgatggt
     4501 ttgcatataa cgggcaggta acgcggcact gacgcttgcg gtaaacagca tcagcaggag
     4561 aataacagct tgtttgtaca tgaaaaaata accgagggtg aaacaacgct tttattgtag
     4621 caaaagcgtc atcagaatcg cgggttaata ctcatacaaa ttatgtttga ggacgaaagg
     4681 cggatttttt tgttgctggt gccaaagact actcacctcc ggcgcaccca gttcgacaat
     4741 acgctcgcga aactctgcgc tggataacgt ttcacggtag gccagcatct gctcaattaa
     4801 cggtaacgtg acgccagaaa tcacttcgca gcgggaatgt ttatggctta atagcgaagc
     4861 cacgcggtac ggcggcgctc cggctatatc ggttaagaag atgacacctt caccagagtc
     4921 cgcttcatgc agagcatcgc acatcatgcg gctgagcata ttagagctta atccgcgcca
     4981 gaaattcacg gcccggcact gaagaagcgc cccatgtttt ttctccagcg catccagtat
     5041 ctcttgcgcc cggtcatcgt gacaggtaat tacccaacct aacatcgcct tcctccttag
     5101 ccgaaaatca gtctaccctg accattgttg ataaagggtg atagccgtca aaactcctct
     5161 cccctaaatc cagaagagag gaaatagcct tagctacgca aaccacgtcc acgttggatc
     5221 agcgaccaac agatcaaata aaacgccaca ataaagacca ccagtacacc gaaggtagtg
     5281 accagcggaa catcattgat accgaggaag ccgtagcgga aaccactgat catataaacg
     5341 attgggttca gatgcgacag cccttgccag aacggcggca acaaagtcag tgagtaaaag
     5401 accccgccca aatacgtaag tggcgttaac acaaaggttg gcaccaggct gatgtcatcg
     5461 aacgttttgg caaacacgcc gttcagcaaa cccgcaaggg agaataacac cgccgtgagc
     5521 accagcgtta aagcgacaaa tacccacgaa tgcacctgaa acggcacaaa aaacagtgaa
     5581 attgccgtca ccagaatgcc aacaaacaga ccacgcgcca cgccaccgcc gacatatccg
     5641 gcaataatga cgtgagtcgg aaccggcgct accagcagct cttcaatatt acgctggaat
     5701 ttggcaccaa aaaatgatga cgcgacgtta gcgtaggcgt tggtgattac cgacatcatg
     5761 atcagccccg gtacaatgaa ctgcatgtag ctgaagccat gcatatcgcc aatacgcgaa
     5821 ccaatcaggt taccgaagat aataaagtaa agggtcatgg tgatgactgg cggcaccagc
     5881 gtctgcaccc agatacgcat aaagcgatgg atctctttcg cccagatgct ttttagcgcc
     5941 acccagtaaa gatgcatcat gcgcgatctc cttgtttttc attaaccagt gaaacaaaca
     6001 gctcttccag acggttagct ttgttacgca tacttaatac ctgaatgccc tgctcactta
     6061 actgcgtaaa tacgctgttg atcccctgct cacgcagcac ttcaacttcc agcgtcgcgg
     6121 tatcgaccag tcgatactga tagccatcga gcttcggtaa cgggcttttc ggtgcgagat
     6181 cgagaataaa ggtttctgat ttcagcttcg ccagcagcgc cttcatcgaa gtattttcta
     6241 ccagctcacc gtgctgaata atgccgatat tgcggcacag catttctgct tcttccaggt
     6301 agtgtgtggt gagaatgatg gtggtgcctt tgtcgtttaa atccttcaaa aagccccaca
     6361 ttgagcggcg aagttcgata tccacgcctg ccgttggctc gtcgagaatc agtagtttag
     6421 gttcatgcat taacgcacgg gcaatcatta aacggcgctt catcccgccc gataacatgc
     6481 gagcacgttc gttgcgtttc ccccatagat cgagctgttt aagatacttt tcgctgcgga
     6541 tatacgcttc tttacgctcc acaccgtagt accctgcctg attcaccaca atttgctgca
     6601 cggtttcaaa cgggttgaag ttaaattcct gcggcaccag tcccaactga cgtttagcgt
     6661 tcacgacatc cttctcgaga tcgtaaccaa atacgctgac ccgcccggag gttttattta
     6721 ccagagagct gataataccg atagtggtcg atttcccggc cccgttcggc ccgagaagcg
     6781 cataaaaatc acccgcttcg acctgcaaat ctattccacg aagcgcctga acgccgcctg
     6841 gataggtttt tttaagctgt tgaagttcca gtgcaatggt cataaatttt tacttacctt
     6901 acgttcttac actttatctg tggtttaaat cgtcccggag ttgccctata ttagccaaac
     6961 gtaattattt ggttacaggt cgttaacctc catgaaagac atagatacac tcatcagcaa
     7021 caatgcacta tggtcaaaaa tgctggtgga agaggatccc gggttttttg agaaactggc
     7081 acaagcgcaa aaaccgcgct ttctatggat tggatgttcc gacagtcgcg ttcctgcaga
     7141 acgtttaacc ggtcttgagc cgggcgaact ctttgttcac cgtaatgttg ctaacctggt
     7201 cattcacacc gacctgaact gcctttccgt ggttcagtat gcagtggatg tactcgaagt
     7261 tgaacacatt attatctgtg gtcactacgg ttgcggcggc gtacaagccg cagttgaaaa
     7321 cccggaactg gggcttatca acaactggct gctgcatatc cgcgatatct ggttcaaaca
     7381 tagctcattg ctcggcgaaa tgccgcaaga gcgccgtctg gataccttgt gtgaactgaa
     7441 cgtcatggaa caggtgtata acctgggcca ctccaccatt atgcaatcag cgtggaaacg
     7501 cgggcagaaa gtcaccattc acggctgggc ctacggcatt cacgacggct tgctgcgtga
     7561 tctggatgtc acagcgacca accgcgaaac tcttgagcaa cgttaccgtc acgggatttc
     7621 caatctcaag ctgaaacacg ccaaccacaa ataaaaatgc catgccggat gcaacacatc
     7681 cggcaacttc acacttactc gtccagcaga atcactttgc cgatatacgg cagatgacgg
     7741 taacgctgtg cgtaatcaat gccgtaaccc accacaaact catccgggat cgagaaaccg
     7801 atgaattcta ccgggacgtt cacttcacga cgggacggtt tatccagcag cgtacaaatc
     7861 gccaacgact tcggttcgcg caggcttaag atctcacgca ctttcgacag tgtattaccc
     7921 gagtcgatga tatcttcaac aatcagcacg tccttgccac ggatatcttc atccagatct
     7981 ttgaggattt tcacatcacg ggtggtggac atgccactac cgtagctgga ggcggtcata
     8041 aaatcgactt catgagatac ctgaacttca cgacacaggt ccgccataaa cataaatgag
     8101 ccacgcagca gacccaccag caccatatcg ctgccgctgt ctttgtaacg ctcagtaatc
     8161 tgacgaccca gttcggcgat acgcgcttta atctccgctt cggggatcat tacttctaca
     8221 gtatgtttca tatctctaac catatgattt aaaaataaat cacttcgcac gggcaggttt
     8281 tggcaccgca acaaagcgca agacacgcag tataccagta aaactattca ttcgcggaat
     8341 ctgttaataa agctcgaatt gtgatgacga tcacacatgt taaaacctat aattaattgc
     8401 tactaaaata ttaatgaatt gaaatggtgt ctctttatgg caattaacaa tacaggctcg
     8461 cgacgattac tcgtcacgct aacagccctt tttgcagcgc tttgcgggct gtatctactc
     8521 attggcggag gctggctggt cgcgattggc ggctcctggt actaccctat cgctggcctt
     8581 gtgatgctcg gcgtcgcctg gatgctgtgg cgcagtaaac gtgccgcgct ttggctatac
     8641 gccgccctgc tgctcggcac catgatttgg ggcgtctggg aagttggttt cgacttctgg
     8701 gcgctgactc cgcgcagcga cattctggtc ttcttcggca tctggctgat cctgccgttt
     8761 gtctggcgtc gcctggtcat tcctgccagc ggcgcagttg ccgctctggt ggtggcgttg
     8821 ctgattagtg gcggaatcct cacctgggcg ggatttaacg atccgcagga aatcaacggc
     8881 accttaagcg ccgatgccac acctgccgaa gccatctcac cggttgccga tcaggactgg
     8941 cctgcgtatg gtcgcaatca ggaaggtcaa cgcttttcgc cgctgaaaca gattaacgcc
     9001 gataacgtcc ataacctgaa agaagcctgg gtgttccgta caggcgatgt gaaacagccg
     9061 aacgatccgg gcgaaatcac caatgaagtg acgccgatta aagtgggcga caccctttac
     9121 ctgtgtaccg cgcaccagcg actgtttgcg ctcgatgccg ccagcggcaa agagaaatgg
     9181 cattacgatc ctgagctgaa aaccaacgag tctttccagc acgtaacctg ccgtggcgtc
     9241 tcttatcatg aagcagaaac ggcctcgccg gaagtgatgg cggattgccc gcgtcgtatc
     9301 attcttccgg tcaatgatgg tcgcctgatt gcgattaacg ctgaaaacgg caagctgtgc
     9361 gaaaccttcg ctaataaagg cgtgcttaat ctgcaaagca atatgccgga caccaaaccg
     9421 ggtctgtatg agccgacttc gccgccgatt atcaccgata aaaccatcgt gatggccggt
     9481 tcagttaccg acaacttctc aacccgcgaa acgtctggcg tgatccgtgg ttttgatgtc
     9541 aacaccgggg agctgttgtg ggcttttgat cccggcgcga aagatcctaa cgcgatcccg
     9601 tctgacgaac acacctttac ctttaactca ccaaactcgt gggcacctgc ggcctatgac
     9661 gcgaagctgg atctggtgta tctgccgatg ggcgtgacca cgccggatat ctggggtggt
     9721 aaccgcacac cggaacagga acgttatgcc agctcgattc tggcgctgaa tgccactacc
     9781 gggaaactgg cgtggagcta ccagaccgtt caccacgacc tgtgggatat ggatcttccg
     9841 gcacagccga cgctggcgga catcaccgtt aacggtcaaa aggttccggt catttatgct
     9901 ccagcgaaaa ccggcaacat ttttgtgctc gatcgtcgta acggcgaact ggtggttccg
     9961 gccccggaaa aaccggttcc tcaaggtgca gcgaaaggcg attacgtcac cccaactcaa
    10021 cccttctcgg aactgagctt ccgtccgacg aaagatttga gcggtgcgga tatgtgggga
    10081 gccaccatgt ttgaccagct ggtgtgccgc gtgatgttcc accagatgcg ctatgaaggc
    10141 attttcaccc caccatctga acagggtact ctggtcttcc ctggtaacct ggggatgttc
    10201 gaatggggcg ggatttccgt tgatccgaat cgtgaagtgg caattgccaa cccaatggca
    10261 ctgccgtttg tttcgaaact gatcccgcgc ggtccgggta atccgatgga acagccgaaa
    10321 gatgccaaag gcacgggtac ggaatccggc attcagccgc agtacggtgt accgtatggc
    10381 gtcacgctca atccgttcct ctcaccgttt ggtctgccat gtaaacagcc agcatggggc
    10441 tatatctcgg cgctggatct gaaaaccaat gaagtggtgt ggaagaaacg tattggtacg
    10501 ccgcaggaca gtatgccgtt cccgatgcct gtaccggtgc cgttcaatat gggtatgccg
    10561 atgctgggcg ggccaatctc cacagcgggt aacgtactgt ttatcgccgc tacggcagat
    10621 aactacctgc gcgcttacaa catgagcaac ggtgaaaaac tgtggcaggg ccgtttaccg
    10681 gcgggtggtc aggctacgcc aatgacctat gaagtgaatg gtaagcagta tgtggtgatc
    10741 tccgcaggcg gtcacggttc atttggtacg aagatgggcg actatattgt ggcttatgcg
    10801 ctgccggatg atgtgaagta accataatgc ccggagagat ccgggcatat ttccgaatac
    10861 ggtcttttta taccgtaaac cctaacatca tccccgtatc ttcatgctcc agcaaatggc
    10921 agtgcgccat ataagcatgt tctttcggtg catcgtgatt aaacttcacc agcacttcgc
    10981 tgacattacc ttctacctta acggtatctt tccagcctgc gcgatgagtc gctggcggtt
    11041 tgccattttc tgacaagata cggaactgcg tgccgtgaat atggaacgga tgcagcatca
    11101 tgtcgcccac gccagagata acccaacgtt cgtactgccc tttcgccgcc gcaaacatcg
    11161 gcttattcat atcaaacgcc tgaccgttga ttttattggc atggtggaaa tcgaacttcc
    11221 cgccgtggtt catatgattc atattgccgt gccccatatg gcccatcatc tggctgtgat
    11281 ccatcccggc catcgcctga tcgccatatt tctccatcag catctgcatc cccatcatat
    11341 cgagcatcgg gtccatagag agttgcagct tacgtaccgt cagcccttcc agcgaaggta
    11401 atgctggcag gctacttaat gtgtctggca aagcaccaga ggcactaatg gcaatcggct
    11461 gaatccgcat taccggatgc ggcttatcaa aaggcgcaat cgccattccc atctggctga
    11521 ccggcagcgt caccaggtca aaggggttgt tgtcgttaac ctccaccagc acttcaaaac
    11581 gctcgcccat cagcaccggc agctcgctca ccttcaccgg ttcaggtagt agaccaccgt
    11641 cgctggcaat cacatacagc gggcgattgt cgctggtggc gaaattgagc gagcgggcgt
    11701 tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg ggcagcgtgt tgtgggtaga
    11761 ttgcaccgtt ggtcagcaac gtatcgccaa accagcccac ggcggcggtc atcacatcca
    11821 gttgataatc aatctgcccg tcagcgctaa atttcttatc ctgaacgatc accggaacat
    11881 catcgatacc ccactgtttt ggcagcatta atttcaggat ctcgtcatct tcaatcacca
    11941 ccagaccagc cagccccatc gccacctgtc gcccggtctt gccatgttga tgtggatgga
    12001 accagcaggt agcggcaggt tgatcaacgt tcaacgtcac cgagcgcttg ccacctggcg
    12061 gaataattcc ctgcgggccg ccgtcgactt cacccggtac ttccagcccg tgccagtgca
    12121 acgtcgtctc ttccgtcagt tggttataga tatcaaccgt taccgctttg ccgcgctgta
    12181 atttcaccgc cggccccagc agattgccgt tatagcccca ggtagttgcg gttttcccgc
    12241 caaaggtgga ctggcctgcg ccaatagtta actgaatgcg attacgggca tcggtcgtga
    12301 gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa tactgcgcgg ctccacagcg
    12361 gtaaagccga agccacgccc agcgcgacgg aatattttaa gaaatcacga cgttgcatag
    12421 ttatttcctt attcttaagc aggcgaaaca aaatcaaacg ttgagcatag tccttcccct
    12481 tacgggaagg tcaagccgca gacacaattt aatcgacaat aatggtcgtc gcctcgctcg
    12541 cagtgtgcta acgtttatct tctttaagcc cctggtagaa gcaatgaaga cgtttttcag
    12601 aacagtgtta ttcggcagcc tgatggccgt ctgcgcaaac agttacgcgc tcagcgagtc
    12661 tgaagccgaa gatatggccg atttaacggc agtttttgtc tttctgaaga acgattgtgg
    12721 ttaccagaac ttacctaacg ggcaaattcg tcgcgcactg gtctttttcg cccagcaaaa
    12781 ccagtgggat ctcagtaatt acgacacctt cgacatgaaa gccctcggtg aagacagcta
    12841 ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa tgcaaagccc tggcccgcga
    12901 ttccttaagc ctgcttgcct acgtcaaata atccctgata cctttttgta gaaatattga
    12961 ccgtgcatcc acggtcaatg ttagctatta tgttgcgccc tttttttacg ggtgttaaca
    13021 aaggaggtat caacccatgg ccgaaaaaaa acagtggcat gaaacgctac acgaccagtt
    13081 tgggcagtac tttgcggtag ataacgttct gtatcatgaa aagaccgatc accaggatct
    13141 gatcattttt gagaatgccg catttggtcg cgtaatggcg ctggatggcg tagtacaaac
    13201 caccgaacgc gacgagttta tctatcatga gatgatgacc catgttccgc tactggccca
    13261 cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac ggtgccatgc tgcgtgaagt
    13321 gacccgacat aaaaacgttg agtcaatcac gatggtggaa atcgatgcga gtgtcgtgtc
    13381 gttctgccgt cagtatctac ccaaccataa cgctggtagc tacgacgatc cgcgctttaa
    13441 actggtgatc gacgatggcg tcaatttcgt taatcaaacc aaccagactt ttgatgtcat
    13501 tatctccgac tgcaccgatc ctatcggtcc cggcgaaagt ctgttcacct cagcatttta
    13561 tgaaggttgt aaacgctgcc tgaatcctgg cggtatcttc gtcgcacaaa acggcgtctg
    13621 ctttttacag caggaagagg ctatcgacag tcatcgcaaa ctcagccatt acttcagcga
    13681 cgttggcttt tatcaggcgg cgatcccgac ctattacggc ggcatcatga cctttgcatg
    13741 ggcgacagat aacgatgcct tacgccatct ctcaaccgaa attattcagg cgcgttttct
    13801 cgcttctggc ctgaaatgcc gttattacaa tccggcaatc catacggcag cttttgcctt
    13861 acctcagtat ctgcaagacg cactggcttc acagccgtcc taaggagaag ataagaaatt
    13921 gaaaaaactg aaactgcatg gctttaataa tctgaccaaa agtctgagtt tttgtattta
    13981 cgatatctgc tacgccaaaa ctgccgaaga acgcgacggt tatattgctt atatcgatga
    14041 actctataat gccaaccgtc tgaccgaaat cttgtcagaa acctgttcca ttatcggggc
    14101 caatattctt aacatcgccc gccaggatta cgaaccacag ggtgccagcg tcactattct
    14161 ggtgagtgaa gaaccggttg acccgaaact catcgacaaa acagaacacc ccggcccact
    14221 gccagaaacg gtcgttgccc atctcgataa aagtcatatt tgcgtacata cctacccgga
    14281 aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat attgaagtct ctacctgcgg
    14341 cgtgatttct ccgctgaagg cgctgaatta cctgatccac cagcttgagt ccgatatcgt
    14401 aaccattgat tatcgcgtgc gcggttttac tcgcgacatt aacggtatga agcactttat
    14461 cgaccatgag attaattcga ttcagaactt tatgtctgac gatatgaagg cgctgtatga
    14521 catggtggat gtaaacgtct atcaggaaaa tatcttccat accaagatgt tgcttaaaga
    14581 gttcgacctt aagcactata tgttccacac caaaccggaa gatttaaccg acagcgagcg
    14641 ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag atttattacg ggcgcaatat
    14701 gcccgctgtt taacggctct ggcggagctc gcgggctccg ccagatttat ttacttttgc
    14761 tgcacgaaat tacgataagc agccaccacc tgcagaaaat cctcaacgcc gcatagcgac
    14821 aggctttctt cgtcgtagta gttcatcccc tcttccattt catcgccagc gaattccagt
    14881 tgattggcgc gaaccatcac ttcttcgccg tccatccata gcgtgtactc atgccctgcc
    14941 cgctgccagg aacgttcgct acctttgagc gtgcgcgcgg cgtcttctac ttcatcaagc
    15001 aaggccaggt tttctttcac ctcttcgtta aaccagtgcc cgaccacttc atgccccatg
    15061 gacatacgca cctttaccac tccggtaata tcacgcagaa attcgtaatc catagtgtgt
    15121 tcctctgctc ccggcaatgc gccattactg cattgcacct taggtttaat tatcgcagca
    15181 ctatgcaaga ttaaaaggtg gaaaagaata aggaataaaa ataaaccccc tttccttgag
    15241 cagaaagggg gtttaaagaa gtgttacttg tcaggtattc aggagaacac ctgagctatt
    15301 ttcaggctgt tacaccgcag tctggaaaat caccccgtcc gctttttcgg tgtactgaga
    15361 aagctggttg aagttcaggt aacggtaagt atcaacggca gttttatcca cctgtgccac
    15421 gtaggtctgg tactcttccg gcgtcggtag tttgccaatc agcgccgcaa cagcagccag
    15481 ttccgcagaa gccaggaaga cattcgcgcc agtacccaga cggttcggga agttacgggt
    15541 agaggtggaa accaccgtcg caccgtctgc tacacgcgcc tggttaccca tacacaggga
    15601 acagccaggg atctcgatac gcgcaccgct cttaccgaag acgctgtagt agccttcttc
    15661 ggtcaactgc gcggcgtcca tacgggttgg cggtgccacc cacaggcggg tcggcaactg
    15721 gcctttatgc gcatccagca gtttacccgc agcacggaag tgaccgatgt tggtcatgca
    15781 ggaaccgata aacacttcgt cgatcttctc accctgtacc gcagacagcg gacgtgcgtc
    15841 atccggatcg ttcggagcac acaggattgg ctctttaata tccgccagat cgatgtcgat
    15901 cactgccgcg tattccgcat ctgcatcggc ttccagcagc tcaggattcg ccagccattt
    15961 ttccatgccc tgaatacgac gttccagggt acgacgatcg ccgtaacctt ccgcgatcat
    16021 ccacttcagc aggacgatgt tggagttcag gtattcgatg atcggttctt tgttcagctt
    16081 gatagtacaa ccagcggcag aacgctcggc ggacgcatcg gtcagctcaa aggcctgctc
    16141 aactttcaga tccggcagac cttcaatttc cagaatgcga ccagagaaga tgtttttctt
    16201 gcctttcttc tcaacggtca gcagaccttg tttgatcgca tacagcggaa tagcatgtac
    16261 cagatcgcgc agggtgatgc ccggctgcat tttgcctttg aagcgcacca gaacggattc
    16321 cggcatatcc agcggcatta cgcctgttgc ggctgcaaat gccaccagac cagaacccgc
    16381 cgggaaagaa atgccgatcg ggaaacgggt atgggagtca ccaccggtac cgacggtatc
    16441 cggcagcagc atacggttca gccaggagtg gattacgccg tcacccggac gcagcgacac
    16501 accaccacgg ttcataatga agtccggcag cgtgtggtgc gtgttcacgt caactggctt
    16561 cggatacgcc gcggtgtggc agaaagactg catcaccagg tcagccgaga agcccaggca
    16621 cgccaggtct ttcagttcat cacgggtcat cgggccggtg gtgtcctggg aacctacaga
    16681 agtcattttc ggttcgcagt acgcgcccgg acgaatgcct ttcacgccgc aggcacgacc
    16741 taccattttc tgcgccagcg agaagccgcg gtcgctctca gcgacatctt tcgcctgacg
    16801 gaacacatcg ctgtgcggca gaccaagtgc ttcacgcgct ttggtggtca ggccacgccc
    16861 gataatcagc ggaatacgac caccggcacg cacttcatca atcagcacgt cggttttcag
    16921 ttcgaaggtc gccagcagtt cgccggtttc gtggttacgc acttcacctt tgtacgggta
    16981 aacgtcaatt acgtcaccca tgttcaggtt agagacgtcc acttcaattg gcagtgcacc
    17041 cgcatcttcc atcgtgttaa agaagatggg tgcaatttta ccgccgaggc acaaaccacc
    17101 gccgcgtttg ttcggcacat gtggaatatc atcgcccata aaccacagaa cggagttcgt
    17161 ggcggattta cgcgatgaac ccgtacccac aacgtcaccg acgtacgcca gcgggaaacc
    17221 tttctgttgc agagcttcaa tctgtttgat cggaccaaca acaccaggct gatctggctc
    17281 aataccttca cgggcgtttt tcagcatcgc cagcgcgtgc agtgggatat ccgggcgtga
    17341 ccacgcatcc ggtgccggag aaaggtcatc ggtgttggtt tcgcctgtga ctttgaagac
    17401 ggtaacggtc agtttttcag ccagtgccgg gcgattcagg aaccattcgg catccgccca
    17461 ggactgcatt acctgcttcg catattcgtt gcctgctttg gctttctctt ctacgtcata
    17521 gaagttatcg aacatcagca gcgtgtgaga aagtgctttg gcagcgatcg gtgccagttt
    17581 ggcatcatcc agcgcgtcga tcagcggatg aatgttgtaa ccaccctgca tagtgcccag
    17641 cagttcgatg gctttttccg gagtcagcag tggggatttg gcttcgcctt tcgcgacagc
    17701 agccaggaag cctgctttga cataggcggc ttcatcgacg ccagggggaa cacggttggt
    17761 taacagatct aacaggaatt cttcttcgcc cgcgggcggg tttttcagca gctctacaag
    17821 tgcggccatt tggtttgcat ccaggggttt gggcgcaatc ccctcagcgg cacgctcagc
    17881 tacgtgctta cggtattctt ctagcacgac ggttctcctc gctctcattg tcatagtgcg
    17941 gcaggcgatt ctcttcacgc tcctgtgaga cagcagtttg tagggtaaat gcccagtacc
    18001 gcaacgggca gaatagcagg attttgacga tgtgttaatc tgtttacaaa aaagcaacat
    18061 aaaaaagtgg ctgaatcgtt aaggatggtc tagagattgt ttccctccgt aaaaacttcg
    18121 ttaattcgca tggtgataat cacctttctc aacgaatccc aagattagtc aaaatttaaa
    18181 ctaccgcctc tttatactcg gattctcagc acctgcgggt ggcagttcgc cgaccattgc
    18241 gatttccttg agattcgaat tatgaaaatg actttgccgt ttaaacccca tgtgctggcg
    18301 ctaatttgca gtgccgggct ttgtgccgcc tctgccgggc tatatataaa aagccgcaca
    18361 gtggaagcgc ctgtggaagc gcaatcgaca caacagaatg cgcctgacat ctccgcagtt
    18421 acgcttcctg caacggtttc cgcacctccc gtaacacccg ccgtcgtcaa atctgcattc
    18481 agcactgcac aaatagatca atgggtcgcg cccgtcgcgc tgtatcccga cgccctactt
    18541 tcgcaggtgc tgatggcatc aacctatccg acaaacgttg ctcaagcagt gcaatggtcg
    18601 cacgataatc cacttaaaca aggcgatgct gctattcagg cggtatctga ccagccctgg
    18661 gacgccagcg tcaaatcact ggtggccttt ccacagttga tggcattgat gggcgaaaac
    18721 ccgcaatggg tgcaaaacct gggcgatgct tttctggcgc agccgcagga cgtgatggac
    18781 tcggtacagc gattgcggca actggcgcaa caaaccggct cgctgaagtc atcaactgaa
    18841 caaaagatca tcacgacaac gaaaaaagtc gtaccggtaa atcagcccgc caacgcccct
    18901 gtcacacaat caaataccgt ttctacgtcc agccccgtcg tcgcagaacc tgcaccgacc
    18961 gtaataacca ttgagccagc caatccggat gtggtttata ttccgaacta caacccaaac
    19021 gtggtttacg gcagttgggc caataccgct tatccgccag tttatctgcc accaccagcc
    19081 ggagaaccgt ttgttgacag ctttgtgcgc gaattcggct atagcatggg cgttgctacc
    19141 acatacgcac tattcagcag catcgactgg gacgacgagg atcatgacca tcatcatcat
    19201 gacgatgatg attatcatca ccacgatggc ggtcatcgtg acggtaatga ctggcaacac
    19261 aacggcgaca acatcaatat cgacgtcaat aatttcaacc atatcaccgg tgagcatctt
    19321 actgataaca atatggcatg gcggcacaat ccaaactacc gtaatggtgt gccctatcat
    19381 gatcaggata tggcaaagcg gtttcaccaa accgatgtca acggcggcat gagcgccacg
    19441 cagttacctg ctctatcgcg cgacagtcag cgtcaggcag cagcaagtca gtttcagcaa
    19501 cgaacataca ctgcgccagt cattacgcga tatactcaac gtcaggctgc ggcacagcga
    19561 tttaatgaag cggaacatta tgggagctat gacgacttcc gcgagttcag ccgtcgccaa
    19621 ccactgaccc agcaacaaaa ggacgccgct cgtcaacgtt atcagtcggc ctcgcctgag
    19681 cagcgccagg cagtccgcga gaaaatgcag actaacccac agaaccagca gcgaaaagag
    19741 gcagcgcgtg agcgtattca gtctgcaact cccgaacagc gccaggtgtt taaggaaaaa
    19801 gtacagcagc gcccactgaa ccaacagcaa cgtgataacg cccgccagcg tgttcaatca
    19861 gcatcacctg agcaacgtca ggtttttcgg gagaaagttc aggagagccg cccacaacgt
    19921 ctaaacgaca gtaaccgtac tgcccgattg aataatgagc aacggtcagc agtacgcgaa
    19981 cgtctctctg agcgcggagc aaggcgactg gaaaggtaaa ttacagacgt aaaaaaagcg
    20041 gcgtggttag ccgctttttt aattgccgga tgttccggca aacgaaaaat tacttcttct
    20101 tcgctttcgg gttcggcagg tcggtaatgc taccttcgaa cacttctgcc gccaggccca
    20161 cagactcgtg cagagtcggg tgcgcatgga tggtcagtgc gatgtcttca gcatcacaac
    20221 ccatttcgat tgccaggccg atttcaccca gcagctcgcc gccgttagta ccgacaatcg
    20281 caccaccgat cacacggtga gattctttgt cgaaaatcag cttggtcata ccgtctgcgc
    20341 agtcggaagc gatagcacga ccagaagcag cccacgggaa ggtggcagtt tcatagctga
    20401 tgcctttctc tttcgcttct ttctcggtca gacccaccca tgcaacttct ggttcggtat
    20461 aggcgatgga cgggataact ttcggatcga agtagtgttt cttaccggcg ataacttcag
    20521 cggcaacgtg accttcgtga acacctttgt gtgccagcat cggctgaccg acgatatcgc
    20581 cgatagcaaa gatgtgcggt acgttagtgc gcagctgttt gtcaacgcgg atgaaaccac
    20641 ggtcgtccac ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc ggcacacgac
    20701 caatcgctac cagcacggcg tcgtaacgct gcggttcagc gggtgctttt ttgccttcca
    20761 tcgtcacata aataccgtct tctttcgctt caacggcggt aactttggtt tccagcatca
    20821 ggttgaattt cttgctgata cgcttggtga agactttaac gatgtctttg tcagctgccg
    20881 ggataacctg gtcgaacatt tcaaccacgt caatctgtga acccagcgcg tggtatacgg
    20941 tccccatttc cagaccgatg ataccgccac ccattaccag caggcgttct ggtacttctt
    21001 tcagttccag cgcgtcagtg gagtcccaga tacgcggatc ttcatgcgga ataaacggca
    21061 gttggatcgg gcgagaaccc gctgcaatga tcgcgttgtc gaagttgatc acggttttgc
    21121 cgttctcacc ttcaacttcc agggtgttag ccccggtaaa tttacccaga ccgttgacca
    21181 ctttgacttt gcggcctttc gccataccag ccagaccacc ggtcagctga ttgatcactt
    21241 tctctttcca ggtacgaatc ttgtcgatat cggttttcgg ttcgccgaag acgatgccgt
    21301 gttcagccag cgctttggct tcttcgataa cttttgctac gtgcagcagt gctttagaag
    21361 ggatacagcc gacgttcagg caaacaccgc caagggtgtt gtaacgttct acgattacgg
    21421 tttccagacc taaatcagcg caacggaagg cagcggagta acctgcgggg cctgccccaa
    21481 gtaccacgac ctgagttttg atttcagtac tcatcatgac ctctatatat ttatctccgg
    21541 cggtcatacc cgtcgtcttt caggccgcag cggcgttagc ttcacgccct caccccagtc
    21601 acttacttat gtaagctcct ggggattctg gcgcttgcta ccttgctgca cccagaaatc
    21661 catagggtat gtgaagtaat accctaacca ccaccgggtc gttctatccg tcggtatttt
    21721 acaaaattgt taacaatttt taaacaacaa acggcaaccg atttgtctat tcgctaataa
    21781 cctcaataca ttcatgag
//
LOCUS       DAPOZO010000082        21674 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPOZO010000082 DAPOZO010000000
VERSION     DAPOZO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.082"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..751)
                     /locus_tag="ROI21_003405"
     CDS             complement(<1..751)
                     /locus_tag="ROI21_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDX5112288.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQIATDDDEIAALTIGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLK"
     gene            1067..1978
                     /locus_tag="ROI21_003406"
     CDS             1067..1978
                     /locus_tag="ROI21_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDX5112289.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(2021..3172)
                     /locus_tag="ROI21_003407"
     CDS             complement(2021..3172)
                     /locus_tag="ROI21_003407"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDX5112290.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPHEFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(3186..4781)
                     /locus_tag="ROI21_003408"
     CDS             complement(3186..4781)
                     /locus_tag="ROI21_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDX5112291.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(4925..5683)
                     /gene="aroD"
                     /locus_tag="ROI21_003409"
     CDS             complement(4925..5683)
                     /gene="aroD"
                     /locus_tag="ROI21_003409"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDX5112292.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVDSVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(5714..6580)
                     /gene="ydiB"
                     /locus_tag="ROI21_003410"
     CDS             complement(5714..6580)
                     /gene="ydiB"
                     /locus_tag="ROI21_003410"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDX5112293.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(6592..7857)
                     /locus_tag="ROI21_003411"
     CDS             complement(6592..7857)
                     /locus_tag="ROI21_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5112294.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIALDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(8084..9298)
                     /locus_tag="ROI21_003412"
     CDS             complement(8084..9298)
                     /locus_tag="ROI21_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5112295.1"
                     /translation="MKNPYFPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRATT
                     LLMLYTFISFTALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(9397..9753)
                     /locus_tag="ROI21_003413"
     CDS             complement(9397..9753)
                     /locus_tag="ROI21_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDX5112296.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKYAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(9889..9996)
                     /gene="rprA"
                     /locus_tag="ROI21_003414"
     ncRNA           complement(9889..9996)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ROI21_003414"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(10182..11294)
                     /gene="ydiK"
                     /locus_tag="ROI21_003415"
     CDS             complement(10182..11294)
                     /gene="ydiK"
                     /locus_tag="ROI21_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDX5112297.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEVLEELGEIEKPNK"
     gene            11683..14739
                     /locus_tag="ROI21_003416"
     CDS             11683..14739
                     /locus_tag="ROI21_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDX5112298.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTAIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCRDL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDVRSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            14736..15146
                     /gene="menI"
                     /locus_tag="ROI21_003417"
     CDS             14736..15146
                     /gene="menI"
                     /locus_tag="ROI21_003417"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDX5112299.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            15246..15434
                     /locus_tag="ROI21_003418"
     CDS             15246..15434
                     /locus_tag="ROI21_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDX5112300.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            15983..16351
                     /gene="sufA"
                     /locus_tag="ROI21_003419"
     CDS             15983..16351
                     /gene="sufA"
                     /locus_tag="ROI21_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDX5112301.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            16360..17847
                     /gene="sufB"
                     /locus_tag="ROI21_003420"
     CDS             16360..17847
                     /gene="sufB"
                     /locus_tag="ROI21_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDX5112302.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGALQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            17857..18603
                     /gene="sufC"
                     /locus_tag="ROI21_003421"
     CDS             17857..18603
                     /gene="sufC"
                     /locus_tag="ROI21_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDX5112303.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGAVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            18578..19849
                     /gene="sufD"
                     /locus_tag="ROI21_003422"
     CDS             18578..19849
                     /gene="sufD"
                     /locus_tag="ROI21_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDX5112304.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAHSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINIAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            19846..21066
                     /gene="sufS"
                     /locus_tag="ROI21_003423"
     CDS             19846..21066
                     /gene="sufS"
                     /locus_tag="ROI21_003423"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDX5112305.1"
                     /translation="MTYSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            21079..21495
                     /gene="sufE"
                     /locus_tag="ROI21_003424"
     CDS             21079..21495
                     /gene="sufE"
                     /locus_tag="ROI21_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDX5112306.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
BASE COUNT         5630 a         5265 c         5463 g         5316 t
ORIGIN      
        1 ttttcagatg ttccgccagc tcggcaatgg cctccggcga atcgttatcg agaatgatgt
       61 gcttacgttc tgtttgcggc ggtacgcgaa tgccggtcag ttcagcaagt ggtgcgctct
      121 ggctccagcc aatgtcactt gcctgccact gatttaccgg ttttttaccc gcgccgagaa
      181 tggctttcat cgaaggaatg cgtggcatgt taatatcgga ggtgacgcag agcacggctg
      241 gaacagagag ctcaataact tcaacatcat cttcaagcgt gcgttcaatc accagtgtat
      301 tgccctgacg ctgaatagca ctcactgcat taatcaccgg aagttgcaga atttctccga
      361 ccagcaagcc aacctgctgg gcataaaggt cgccggaacc ttcaccaaag atcagtaaat
      421 cgaagccgat cttttcaact gctgccgcca gcgcctttgc ggtatcgaga ggcagtgcat
      481 gttcaagttg cgcatcctgc accaaataca ggctatgtgg cccgcgggat agcacgtctt
      541 tgcgcacttt cgagttctgc aacaatgagc cgccaatggt cagcgcggct atctcatcgt
      601 catctgttgc aatctggctt gcagcttcaa tggcattgag atcgaactgg ctgattttgg
      661 cgtcggcatt gtcgaaattc agggtatatt ctggagtgac aacaatgtcc tgttcttcag
      721 gcaccagctt aaagcaggtt attattttca tgatttctcc tgtgagtcgc atacggaacc
      781 atcaccgatg gaaggtaaaa atggcctgaa aatagtgtgc gatagcagtg cggggttttc
      841 gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa aaaatgcgat cgctaaaaca
      901 aaagtgcaat ttgcggcaat gttgttattc catgaccgta caatcttgca ttcagtgtaa
      961 ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc gcaatacacg ccgtatttta
     1021 tccggcatat attgtttttg cttgctcatg gaatagggag gttgtcatgt atcaacgctg
     1081 ttttgataac gccagcgaaa cgctgtttgt cgccggtaaa acgccacggc tttcacgttt
     1141 tgcatttagc gatgatccaa aatgggagtc cggacatcac gttcatgaca atgaaaccga
     1201 gctgatttac gtcaagaaag gggtagcaag gtttaccatc gattcttcgt tatatgtcgc
     1261 gcacgccgat gacattgtgg tgatagaacg tggcaggctg catgcggtgg cctctgacgt
     1321 taacgatccg gcaacgacgt gtacctgtgc gctgtacggc tttcagtttc agggggctga
     1381 ggaaaatcag ctactgcaac cgcattcttg cccggtaatt gccgcagggc agggaaaaga
     1441 agtcattaaa accttattta atgagctaag tgtgattttg ccgcaaagta aaaatagcca
     1501 aacatcttcg ttatgggacg catttgccta tacgttagca attctttact atgaaaactt
     1561 taaaaatgct tatcgttcgg agcagggata tattaaaaaa gatgttctga taaaagatat
     1621 tcttttctat ctgaataata actatcgcga aaaaatcact ttagagcagt tatcgaaaaa
     1681 gtttcgtgcc agcgtcagtt atatttgcca tgaatttacc aaagagtatc gcatttcccc
     1741 tattaactat gttattcaac ggcgtatgac ggaagcgaaa tggtcactca ctaatactga
     1801 attatcacag gcagagatct cctggcgtgt gggttatgaa aatgtcgatc actttgccaa
     1861 actgtttttg cgccatgtcg gctgttcgcc cagcgattac cgcaggcaat ttaaaaactg
     1921 ttttgcggaa caagaaatct tatctgaatt tcctcaaccg gtaagtcttg ccggataaat
     1981 ctggcggcgc agtttgttaa gaagctgcgc cgcctgcaga ttatttgttc tgataatctt
     2041 tcaggatctg ccgacccgct acgtaaatca taatttcgtc tgtaccgccg ccgatacgtt
     2101 cacaacggac atcacgccag aagcgagaga cgcgcgcctc atcggtatag cccagaccgc
     2161 ccatgatttg aatcgcatca tcaataactt ccattgcggt acgtgcgcag tacagttttg
     2221 ccagcgccgc gctggtgcgc agtgactgat gctgatcggc ttgccatgcc actttcaaca
     2281 ccatgttgcg catgttgtcg atcttaatcg ccatcagcgc cagtttttcc tggatcatct
     2341 ggttatgacc aatgggctta ccaaaagcga tacgttggtt ggcatagcgg gcggcatctt
     2401 caaaggcaca ctcggcaaag ccggtgctgc gcgcggcgtt gatcaggcgc tccatctcaa
     2461 agttgtacat cacattgagg aaacccattc cttcttcgcc caccatgtcg ctctcttcaa
     2521 cctccacgtt gtcgagatag acttcgcagg tgctgagcat atgccagccg attttatgca
     2581 gcgggttaat cttaatgccg ggcttactgg agtcgaccca ccacagggtg aaggcttttt
     2641 tgggatcttt cggttgcgga tcgcgcgcca acaccagcat atacgggtac tctttcgcac
     2701 cggtaataaa ggttttttgt ccgttgatgt agaccttgcc gtttttacgc gtgtaagtgg
     2761 tagtggcact gttgttatct gagccagcac ctggttccgt caacgccagg gcataggcgg
     2821 gatcacccgt ttccagggtg ctttctgccg ttttacgtag ctgctctgca gaaccgaaac
     2881 ggcgcatact gtgaatacat tgaccgttgg tgatcaaaaa tgccggagca ccgcattttg
     2941 acacttccat cagcgccagc atttgggtga cgtaatccgc agggatacca ccaaattctt
     3001 ccggcacgcc aagcatggaa ataccgttat ccgccagcgc ccgcataaac tcatgcggat
     3061 atgtcccgtt ttgatcgcag gtgcggaaat actcttccgg aaagttagtc gtaatcagtt
     3121 cgcgaatact ggccagcagc agttcttgtt cttcagttaa agaaaaatcc atcgtgatac
     3181 tcctgttaat gagccgcttc aggcaggaca aaacccatcg ccgcatcgat aaataatctt
     3241 tcgtccatca gtttgagttc tggcgaaatc actggggtga agtccatttt gtcgagaata
     3301 tctttttgta aatcgacgcc aggggcgatt tcgattaaat gcaggccgtc ttctttcagc
     3361 gtgaatacag cacgttcggt gatgtaacga acatccagtt cccgttcgag cgcgattttt
     3421 ccgctaaaag taatttccgg tagttcccga ataaatttat ttacccgtcc ttcctggacg
     3481 atatgcaatt tgccatcggc aatttctgtt tttaaactgc ccgcagttaa cgtgccgcaa
     3541 aaaataattt tcttcgaggt ggcactgata tcaataaatc caccggtacc catgattttg
     3601 ccattgaatt tatgcacgcc gacgttaccg tgctggtcga cttcagcaaa actcaaataa
     3661 caaacatcca gaccaccacc gtgataaaaa tcaaactggg acgtcatatc cagaatggca
     3721 cgggtattca cgttcgcgcc aaaggcgatc ccctgtgaag taattccgcc aatcggacct
     3781 gtttctaccg tcagaataaa gtcatcagca caaccttctt cccgggcgac caggccaata
     3841 ccgtcagcaa taccgacgcc gacattcccc accgcgcctt tacgcatttc gaataatgca
     3901 cgcctcgcaa ctaatttacg ttggtttagg ggcaggctaa gtttggtgct gtcatcaagg
     3961 gtgaagtcac cagaaataaa gcggttaacc ggcgcaccgc catacagttg gctttgatcc
     4021 ggatcgacca ccacaatatc caccagataa cccggaatac ggacagattt aggatgcagc
     4081 gtggctttct taaccatttt ctgcacctgc atcatcacaa taccgccgtt attgtgcacc
     4141 gcctgggcaa taaccaatgc gtcgagatac atcacctcat cttcaaaagt ggcgtagcct
     4201 tcactgtcgc aggtggtagc gcgaatgaag gcgatatctg gcgcaatcgc tttgtaatag
     4261 agatattctt tgttatcaaa ctcgaccagt ttaatcaggt cttctttcgt gacttcattc
     4321 agtttgccgc cttgctggcg tggatcgaca aatgtcccga tgccaatatc actaataata
     4381 ccaggctggt gggctgcggc ggcgcgtaag gtttgtgtaa gtacaccttg tggatagtta
     4441 taagcaataa ttttattttg ttctgcgagt tcagaaatac gcggcgattg tccccagtga
     4501 ccacataatg cccatttcac cagaccttct tgcgccagag gactaatacc acggtcggcg
     4561 cgatcgccaa gccctgtcgg actaataatc gataaattac gtggcgtttg agtctgttta
     4621 tatttatcag caagagctgt aattaacgtg gtggcttcca gaataccgcc gccagcgcct
     4681 aacacacaaa gtgttgcttc gtcgggaata taattcaccg cttcctgtgc cgacaggacc
     4741 ggcactcgtc cattaatacg aggtggtttt acaggtttca ttgttaattc ctgattcaga
     4801 tgtgattttc cctacgccag atggagaatt agcgcacaga gactcacgaa atatttttat
     4861 taaatacggt taaatatatt ttttaagttc ggcggggagg gtgttcccgc cgaaatatta
     4921 ttgcttatgc ctggtgtaaa atagttaata ccgtgcgtaa atcatttacc gagatttgcc
     4981 ctggtgcaga ggcttttttt accgcaccaa aagttgccgc cgagccaaat acttcaccag
     5041 ccagacgaga aattacgcca gtttttgcca tcgacatcgt gatgattgga cgatcggcat
     5101 actgctcctg catctccagg gtcgcggtaa gcaacgtcag cacatcgctg gtactttgcg
     5161 gcatcagcgc aatcttagga atatcggcgt cgaaggactg cattttgcgc agacgggcaa
     5221 tgatttcttc ggcttccggc gttttatgga agtcatggtt ggacatgaca actttcacat
     5281 catgcgcgtg ggcgtaggcg acggtttctt taacctgatc atcgccggta aataactcca
     5341 gatcgatcat atcaaccagg ccgctgtcga tggctgcacg attgagtgca atataagcct
     5401 cggtggaaat cgcctgctcg ccgccttctt tggcactgcg gaaggtaaac agcagcggtt
     5461 tttctggcat ggtttcacgg agaatttttg ccgccgccat gacagaatcc acattggaga
     5521 ggtcggcata gtggtcaaca cgccattcca gaatatcaaa gtccgcttca cgataggcga
     5581 gagcttcaga tttcacgctg gcgatatctt tcgccatcag cgagacgatg attttaggtg
     5641 cgcccgtacc aatgacgaga tcttttacag ttacggtttt cattttttac cctttctgca
     5701 cgcggtcagc ctgtcaggca ccgaacccca tgacctgttt aacatattcc agagggaaat
     5761 ctttgccagt ccataacgtg aactgttcag ccccttgcca caacaacatg ccgtatccat
     5821 caatcgtttt gcaaccagct tgttgcgcct gctgcaataa cttcgtcata tgcgggttat
     5881 acacgcattc agtgaccaga agtcccggat gtaacagact gatatcatta accaatgatt
     5941 cattctcaag gggtttcata cccacttttg tgccattggt taaaatgtcg gcggaagcca
     6001 gggcttcagc aaaggcttgc tgatcggcga gatcggtaac cgtgacgaca caatcggtgt
     6061 tttcgttaac ccgctgcgcg aaggcgaggg ctttatcgaa gaactcatcc cgacggttaa
     6121 agagtttaat ttcttttaaa ccttcaattg ccccctgcgc gccaatcgcc gttgaggcac
     6181 ctccggcccc taacagcacc atcgttttgc ctttgatatc aaaaccgctc tctttaatgg
     6241 cgcgaatatg gcccgtgccg tcggtgttat agccacgcag atagccatca tcattaacga
     6301 tggtgttgat ggcacccacc agtttggccg ctggcgttaa ttcatcaaca tattcacacg
     6361 ccagttgttt gttcggcatt gatacaccag ttccgcgcat tttgagggct tttaatcctt
     6421 caattgctcc gggaaaacta tcgttatcca cttcaaaagc catataggta aatggcaatc
     6481 ccgctttttc taaggcttta ttctgcattt caggcgataa actgtgacgg atgggatatg
     6541 ccatcagccc aatcaattcg tattttgcgg taacatccat aattaactcc tttaacctct
     6601 atgcttaatt gtgtttaacc gggtgctaaa tttacgctcg ccaaaccgca catcattttc
     6661 aggaataatg aaaacgcggt aatagcggat aaagacaata attgcggtaa tcagggccag
     6721 cagcgcgaaa gtaaaatcga gagcaatgat atattgcagg ccgatgttcg acagataacc
     6781 ggtaatcagc ggaataacaa aattagccag tccacccatc atcatatata tactggtgac
     6841 tttggctttg ctttttggga aaaactctga catgaccgaa acgccgagct gtaaaatgcc
     6901 gccagctgct gagaaaccga taacaaaggc tccggcatta cacaccaatg gagaagggta
     6961 caggtagata atggcagctg ttattgttgc cagtgcagag ttaaatacat tagcccagat
     7021 gggccggacc atttttttca gcagtgcggc aaaaataaag acacagacca acgagcccat
     7081 actgtaataa gagatggttt ttaatgcctc agcttctgac ataccagcaa aagccatcgc
     7141 atatttgggc atccacacca caatcacata aaaggtcgag aatgcggcta caccgaacag
     7201 tactgatgaa acaccttcca gccagactaa cggtttgctg ttcatttgcg gtaattcatt
     7261 agctacgctg gcgtccacca actggctggg gaatttgctt ttcaacagca tcagtgtgat
     7321 cagtacaaac agaatacccg gaataatcag cccatagccg taccagatgt tattgagcaa
     7381 catatagctc accagcattg ggtagaacat ttgcccgaat gacaccatcg ctttaaccag
     7441 tatgaccgcc gaaccagagg ctttcggaaa gcattccatg agcgcggggt agccacccgt
     7501 atccagcgct gagttagcga tacctacgca cactgccaga cagtaggcga gagttaaatt
     7561 cgggcaagcg ggaataccaa agaagaatag cagatacatt attactgcca ttaatatcac
     7621 cgcccgacga ccaaacttat cggagatcac accgaagaat aaaatactga tcaatcgccc
     7681 caaaccgata ccggaaatta aataggcaat gcccgcgttg tcagtggaaa acttttctgc
     7741 cagagatgac atattttggg caagcgtaat aacactaatg ccgtgcagga aatagctgaa
     7801 gtaaatacaa agaacagcca ggataaatgg cgtgctgaaa gccttatttt gagacatttt
     7861 cacagctcct tgctgaaaga taatttcgtt accgtacgta ttgttctccg acggttcaca
     7921 taattactat actgaagatg attttccctg ggtacatggc ataaagttta tgtttccagg
     7981 aatattgcat gtcatagttt gttttttatc gcgattttcg ttagttggcg cagaaagagt
     8041 tatttatccg taagatcttg catgacctga aaagaatatt gcattaccca cccggagcga
     8101 cattttcctt tagcgagtga tgccgcgttt ctttgcggct gcgtaagccg ataaacagtg
     8161 ccagtaaaaa gccgatggca gcgatggcgg tatcgaacca cataatatcg gcaatacttc
     8221 tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg cccgcactgt
     8281 aatagatccc tgtagcttta cctttagcgt aggggaaacg ttcagccatt aatgtcaggc
     8341 caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat attatcacca
     8401 cataaaatgt gggatgcagg cagacggtaa acagagcagt aaatgagata aaggtgtaca
     8461 gcatgagtaa tgttgttgct cgaacggtat tacgaatgag tggagcggta ataaatacac
     8521 aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg tatgacatgc
     8581 ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac gttgccattg
     8641 agatatagcc atataaggta taactggcta aatcgattat tgagcagcga tgttccgtag
     8701 agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg aacgtacaac
     8761 ggtataaaaa cagagcgtta ataaacataa tgcctgcggc aatcataaag gaccaaccga
     8821 accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat tgcccgctgg
     8881 aaacaaatgc tttaattaaa atattggctg tacccggtga gcgtggaaaa gcttccatca
     8941 aactgggata ggtgcctgca tcgagaaaac tgtttgccat tcccgccaga aagccaaaaa
     9001 cataagcgat aatgatgtta ttggtgtgca ggatgccaaa aaagaaggcc atatagcaac
     9061 acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat cctgcaaaaa
     9121 gcaagacact taatcgacca atacccagcg atgagataac tattgagacg cctgcggcat
     9181 tagtctgcca aagcgtctcc agcgaggcca tattcaggct catcaaaatg acgcccatac
     9241 catgcaccag gtaattaaaa tacaacccca gtgcggtagg gaaataggga tttttcatag
     9301 gttctactta gcaaaggtgc tttaaaaagt gaagcgatgt tgtgtcagca tccgttcatt
     9361 attgttgtaa caacgacaat tcctttcttt ggaaaattaa caaaggcgct ccagatcgtg
     9421 tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat tgccatcagg
     9481 gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg
     9541 gttatttatt ttctctatga tggcatgaag atgttttttt ctttgctgac gcatagccaa
     9601 cagttgctcg actacacgat caggaatggc gtatttgcca ttttcccact gacgccagct
     9661 ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca tggcaaaaat
     9721 atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg aaataaacaa
     9781 taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca gtatcttgtg
     9841 caacattatt ggaaagcgtg aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc
     9901 ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact caggggattt
     9961 ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg catacggact
    10021 ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt gcgacaggta
    10081 ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa tatcaattag
    10141 caggggttaa caatcaggcg gcagagccgc ctgatgagaa attacttatt cggtttctcg
    10201 atttcgccaa gttcttcgag aacttcttcc ggttggtcag tcggcggtgg gacttcttcc
    10261 acccacgcgg caaaaagacg ccaggaaacg gctaacagaa ccggaccaat aaacaggccg
    10321 atcatcccga aagcaatcaa accaccaata acgccagaga gaatcaggat cagcggtaaa
    10381 tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc aaccacaccg
    10441 ctccacacta acaataccgt tccccaggtg gtatcgccag tccagtagag ccagataatc
    10501 gccggaatca gtaccggcaa cggtccaagc tggacaaggc aggagaggat cattaacacc
    10561 gtgagcaaag ttgcataagg tacgccggat acggcgaggc cgataccgcc aagcactgcc
    10621 tgtactaacg ctgtcaccac aacacccagc gccaccgcgc ggatagcttg tgccgccagc
    10681 agcacggcgg catctccacg aacgcctgcc agacgggttg caaaatggcg aatgccttgc
    10741 gccacttgtt caccgcgcca gtacagcagg gcgctgaaga gaagcatcag cgcacaatgc
    10801 accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt ggtgccaata
    10861 taaggacgga cttttgccat gatcgccgtc ccccccatat ccagcaagtt gtgccagcct
    10921 gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc gggtaacgtc
    10981 atgtcaccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat actgttaacc
    11041 agcaaggcaa tagggatgat aaacaccatc actaataaca gcgtcatcac cagaacggcg
    11101 agagagcggc gaccaaacat gatcttttgc aaacgtaaca ataccggcca ggtggcgata
    11161 accaccgtac cagcccatgc aaagccgaga ataaagggtt gtacaatcca cagacatgcc
    11221 acaatcatga tggctaaaaa aagcaccgaa agcagaattt gtgcgacatc cctgggctga
    11281 cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg acgcgacgtg
    11341 aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa caggccgatt
    11401 ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact caggcaacac
    11461 gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt cgttcacccc
    11521 agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct gcaactcgaa
    11581 atccatagag taaataaaca tcacctttca gtgcgctatt gcagcagcct gaaagaatag
    11641 tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc acagatttcc
    11701 caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga gcaacaaggt
    11761 tttaccggcg atacggcgac aagttatgcc gatcgtctga caatgtcgac cgacaacagt
    11821 atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga tgtggcgctg
    11881 atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc ccgcggcggc
    11941 ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat gtcccgccat
    12001 atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga ggccggggtg
    12061 ataaaagatc aactcaatca gtacctgaaa ccgttcggct acttttttgc gccggaactt
    12121 tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc cggtcaggga
    12181 tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt gttgttgggg
    12241 ggcgatattc tcgatacgca acctttaccc gtcgaactgg cagaaacgct gggtaaaacc
    12301 aataccgcaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca gcaacgccag
    12361 ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga tctgcgtcat
    12421 gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg ttcagaaggg
    12481 acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa agtgcggcgt
    12541 ctggtgaacg tcaaatatga ctcttttgac tccgcgctgc gtaacgcgcc gtttatggtt
    12601 gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct ggcgcgggaa
    12661 gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa agagatgctc
    12721 gggctgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga gcgagttaat
    12781 gcactctgtg tgcggcttga tgagctgatt gccagccaac aggcgggggt gattggctgg
    12841 caggtgtgcc gcgatctggc gggtgttgaa cgtatctatg cgatgcgcaa aaaagctgtc
    12901 ggcctgctcg gtaatgccaa aggagcagcc aagccaattc cgtttgctga ggatacctgc
    12961 gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct cgacagccac
    13021 ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt ccgtcctgcg
    13081 ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga tgacgtagtg
    13141 gcgcttaccg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg ttttcgcgct
    13201 gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa agtgaaagcg
    13261 gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga aggtctcgat
    13321 gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca gatccccatt
    13381 gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt atgcttcaac
    13441 tttgatgtcc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg gattcattca
    13501 ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga tcgcggcgtt
    13561 gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg gacgttaatt
    13621 gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat acgacttctc acacgaagtc
    13681 aaagaggcaa tgtcgggctg tctggcctgt aaagcgtgtt cgacccagtg ccccatcaaa
    13741 attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg ttatttacgc
    13801 ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat ggcacgcgcg
    13861 ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc ggaaaaacat
    13921 atcggcatgg ttgatttgcc actgctgtca gtcccctcgc tacaacaaca aatggtgggg
    13981 catcgctcgg cgaatatgac gctggaacag cttgaagctc tcaatgcaga gcagaaagcg
    14041 cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcaca agtggtggcg
    14101 gattttgtcc gtctggttga aaaattagga ttccagcctg tgttactgcc attttcgcca
    14161 aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac ggcgaaaaag
    14221 acggcggatt tcctcaaccg tatggcgaag ctggggatgc caatggtggg cgtcgatccg
    14281 gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga acgtggcgag
    14341 tttaacgtct tactggcgaa tgaatggctg gcaagcgcac ttgagtcaca gccagtggct
    14401 acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaagttac cgctttgccg
    14461 ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact ggaaaatgtc
    14521 agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa ccataaaaat
    14581 tcgctcggga tctatgagtt atcctggcat caggcgatgc agcgactgcc gcgtaatcgc
    14641 tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggtcgaagg cacgggggta
    14701 cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacga aaaatcaccc
    14761 tggaagcact gaatgccatg ggtgaaggaa acatggtggg gttgctggat attcgctttg
    14821 aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg acaaagcagc
    14881 ctttcgggtt gctgcatgga ggggcatccg tggtactggc cgaaagtatc ggttccgttg
    14941 ccggttattt atgtaccgaa ggtgagcaaa aggtggttgg tctggaaatc aatgctaacc
    15001 acgtccgctc ggcacgagaa gggcgggtgc gcggcgtatg caaaccgttg catctcggtt
    15061 cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg tgctgttcgt
    15121 cacgattgac gaccgcgatt ttgtgacgtt gtcaccagaa aagtgtgacg catattgctt
    15181 ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct aaaagcgagg
    15241 atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta cgccgtgacc
    15301 agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa cttgaatttg
    15361 ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt gcctggcgtc
    15421 tgggcgtgca ttgatcctga cttaatgccg caatttcgcc aaatcgacct ggattaacct
    15481 ccgtccaggt tcgatgacct tcttaatact gcttatctac ccgcctgata ttgttcaaaa
    15541 ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat tattcttatc
    15601 gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg gggtagaaac
    15661 ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct gcactttttt
    15721 acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac agccttaatg
    15781 ctccttagat aataatcatt atctaacaat gagataccta attcttagcg tgcctgttaa
    15841 cccacacatc agggtctatg cttattaaaa gcaggaaaaa agcatgtttt acggtaaagc
    15901 ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt atacgctgaa
    15961 agcgatgaag tgaggtaaat cgatggacat gcattcagga acctttaatc cacaggattt
    16021 cgcctggcag ggcttaacgc tgacacccgc agccgctgtc cacatacgcg agctggtggc
    16081 aaagcagccg ggtatggtcg gcgtgcgctt aggcgtgaag caaacgggct gcgcgggctt
    16141 tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt ttgaacacga
    16201 cggcgcgaag ctgtttgtcc cgctgcaagc gatgccgttt attgatggca cggaagtcga
    16261 tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag cccagaatga
    16321 atgtggctgt ggcgaaagct ttggggtata ggcggtacta tgtctcgtaa tactgaagca
    16381 actgacgatg tcaaaacctg gaccggcggc ccactgaatt ataaagaagg attcttcacc
    16441 cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg cgcaatttcg
    16501 gcgaagcgta atgagccgga gtggatgctg gagtttcgtc tcaacgccta tcgcgcatgg
    16561 ctggagatgg aagagccgca ctggttgaaa gcgcactacg acaagctgaa ttatcaggat
    16621 tacagctact actcagcacc atcgtgcggt aattgtgacg acaactgcgc gtctgaaccc
    16681 ggcgcgttgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga ggcggcgttt
    16741 gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc cattttcgac
    16801 tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat tattttctgt
    16861 tcctttggcg aggcgatcca cgatcacccg gaactggtgc gtaaatatct cggcaccgtg
    16921 gtgccgggga atgacaactt ctttgccgca cttaatgcgg cggtagcctc tgacggtacg
    16981 tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta ttttcgtatt
    17041 aacgcggaaa aaaccgggca gtttgagcgc accattctgg tggccgacga agacagctac
    17101 gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt acacgcggcg
    17161 gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt acaaaactgg
    17221 ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg tgctttgtgc
    17281 gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc gattacgtgg
    17341 aaatatccca gctgtatttt gcgcggcgat aactccattg gtgagtttta ctcagtggcg
    17401 ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat cggtaaaaac
    17461 actaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa cagttatcgc
    17521 ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca gtgcgactca
    17581 atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg tcgcaataac
    17641 agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca actgttttac
    17701 tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa cggtttctgc
    17761 aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa actcctcgcc
    17821 atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa agatttacac
    17881 gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg tcccggcgaa
    17941 gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc aacgcttgcc
    18001 gggcgagaag attatgaagt gacgggcggc gcggttgagt tcaaaggcaa agatttgctt
    18061 gcgctgtcgc cggaagatcg tgcaggtgaa ggcatcttta tggccttcca gtatccggtg
    18121 gaaattcccg gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc ggtgcgcagc
    18181 tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga agagaaaatc
    18241 gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg tttttccggc
    18301 ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga gttatgtatt
    18361 cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggttgc cgatggcgtg
    18421 aactcgctgc gtgacggcaa gcgttcattc atcattgtta cgcactacca acgcattctc
    18481 gactacatca agcctgatta cgtccatgtg ctgtatcagg gacgaattgt gaaatccggc
    18541 gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac cgaacagcag
    18601 taacgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac gctcaccgca
    18661 agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta aacatgaaaa
    18721 ctggaaatat acgccgctgg aagggctgac caatagccag tttgtcagca ttgcgggaga
    18781 gatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc ggctggtgtt
    18841 tgttgatgga cgttacgtgc ccgcgctgag cgatgcaact gaaggcagcg gatatgaagt
    18901 gagcattaac gacgaccgtc aggggttacc cgacgctatt caggctgaag tgtttctgca
    18961 tttgacggaa agcctggcgc atagcgtgac gcatatcgcc gtgaagcgcg gtcaacgacc
    19021 ggcaaagcca ttgctgttaa tgcatattac ccagggcgtg gcaggtgaag aggtcaacac
    19081 tgcccattac cgacatcatc tggatctggc ggaaggtgcc gaagcaacgg tgatcgaaca
    19141 ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta tcaatatcgc
    19201 agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccgctca gtcaccactt
    19261 tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccaca gtttcctgct
    19321 gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa acagcacgct
    19381 gcggatcaat agcctggcga tgccggtgaa aaatgaggtg tgtgatactc gtacttggct
    19441 ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg tcagcgacaa
    19501 aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca aaacggatgg
    19561 tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata cgaaaccgca
    19621 gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg ggcgtattga
    19681 tgacgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg cccagcagat
    19741 gatcatttac gccttcgctg ccgaactgac ggaagcactg cgtgatgagg ggcttaaaca
    19801 gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac ttattccgtc
    19861 gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt gccgctggct
    19921 tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc cgaggccgag
    19981 ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag cgcccaggcg
    20041 accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc ccgttcggcg
    20101 gaagagctgg tgttcgtccg cggcacgacg gaagggatca atctggtcgc caatagctgg
    20161 ggtaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga gcaccacgct
    20221 aacattgttc cctggcagat gctttgcgca cgcgttggcg cagagctgcg tgtgatcccg
    20281 ctcaatcccg acggtacgtt gcaactggag acgctaccta cgctgtttga tgagaaaact
    20341 cgcctgctgg caattactca tgtctccaac gtgctgggaa cggaaaatcc gctggcggaa
    20401 atgatcacgc ttgcgcatca gcatggcgca aaagtactgg tggatggcgc tcaggcggtg
    20461 atgcatcatc cggtggacgt tcaggcgctg gattgcgatt tttacgtgtt ctccgggcat
    20521 aaactgtatg gccccactgg gattggcatt ctttatgtca aagaagcctt gctacaggag
    20581 atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag tgaaggcact
    20641 acctggacca aagcaccgtg gcggtttgaa gccggtacac ccaataccgg gggcatcatt
    20701 ggtcttggcg cggcgctgga gtatgtgtcg gcgctggggc ttaataacat agccgagtat
    20761 gaacagaatc tgatgcacta cgcgctatca cagctggaat ctgtaccgga tctcactctc
    20821 tatggcccgc agaacaggct tggcgttatt gcttttaatc tcggtaaaca ccacgcctat
    20881 gatgttggca gttttctcga taactacggc attgctgtgc gtaccggaca tcactgcgca
    20941 atgccattga tggcctatta caacgttcct gctatgtgtc gggcgtcgct ggccatgtat
    21001 aacacccatg aagaagtgga tcgtctggtg accggcctgc aacgtattca ccgtttgctg
    21061 ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct gcgtaatttt
    21121 ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg ccagcgtctg
    21181 ccagaattac gcgacgaaga ccgaagccca caaaatagca ttcagggctg ccagagtcag
    21241 gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg tgacagcgat
    21301 gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca gatgacgccg
    21361 caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct gacccaacat
    21421 ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg cgccaaagcc
    21481 gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat tgccggatgg
    21541 tcaatgttta cactgacctg cacaggagtg tttcggtttc aggtgaacat aagcgccatc
    21601 cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac gcgcgtctct
    21661 gcttacactc acgc
//
LOCUS       DAPOZO010000083        21509 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPOZO010000083 DAPOZO010000000
VERSION     DAPOZO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.083"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(11..985)
                     /gene="lepB"
                     /locus_tag="ROI21_003425"
     CDS             complement(11..985)
                     /gene="lepB"
                     /locus_tag="ROI21_003425"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDX5112307.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(1001..2800)
                     /gene="lepA"
                     /locus_tag="ROI21_003426"
     CDS             complement(1001..2800)
                     /gene="lepA"
                     /locus_tag="ROI21_003426"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDX5112308.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGDRVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(2998..3477)
                     /gene="rseC"
                     /locus_tag="ROI21_003427"
     CDS             complement(2998..3477)
                     /gene="rseC"
                     /locus_tag="ROI21_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDX5112309.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLMGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(3474..4430)
                     /gene="rseB"
                     /locus_tag="ROI21_003428"
     CDS             complement(3474..4430)
                     /gene="rseB"
                     /locus_tag="ROI21_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDX5112310.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(4430..5080)
                     /gene="rseA"
                     /locus_tag="ROI21_003429"
     CDS             complement(4430..5080)
                     /gene="rseA"
                     /locus_tag="ROI21_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDX5112311.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(5113..5688)
                     /gene="rpoE"
                     /locus_tag="ROI21_003430"
     CDS             complement(5113..5688)
                     /gene="rpoE"
                     /locus_tag="ROI21_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDX5112312.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(5685..5840)
                     /gene="rseD"
                     /locus_tag="ROI21_003431"
     CDS             complement(5685..5840)
                     /gene="rseD"
                     /locus_tag="ROI21_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDX5112313.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            6096..7718
                     /gene="nadB"
                     /locus_tag="ROI21_003432"
     CDS             6096..7718
                     /gene="nadB"
                     /locus_tag="ROI21_003432"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDX5112314.1"
                     /translation="MNTLPDHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPSALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMYLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(7703..8440)
                     /gene="trmN"
                     /locus_tag="ROI21_003433"
     CDS             complement(7703..8440)
                     /gene="trmN"
                     /locus_tag="ROI21_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDX5112315.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            8572..9906
                     /gene="srmB"
                     /locus_tag="ROI21_003434"
     CDS             8572..9906
                     /gene="srmB"
                     /locus_tag="ROI21_003434"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDX5112316.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(9939..10820)
                     /locus_tag="ROI21_003435"
     CDS             complement(9939..10820)
                     /locus_tag="ROI21_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5112317.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVAKELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            10923..11510
                     /gene="eamB"
                     /locus_tag="ROI21_003436"
     CDS             10923..11510
                     /gene="eamB"
                     /locus_tag="ROI21_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDX5112318.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCVGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRPLNIVLALLLVYCAVRIFY"
     gene            complement(11572..11955)
                     /gene="grcA"
                     /locus_tag="ROI21_003437"
     CDS             complement(11572..11955)
                     /gene="grcA"
                     /locus_tag="ROI21_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDX5112319.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            12260..12949
                     /gene="ung"
                     /locus_tag="ROI21_003438"
     CDS             12260..12949
                     /gene="ung"
                     /locus_tag="ROI21_003438"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDX5112320.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            complement(12997..14034)
                     /locus_tag="ROI21_003439"
     CDS             complement(12997..14034)
                     /locus_tag="ROI21_003439"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDX5112321.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSHPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDEALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            14241..14660
                     /gene="trxC"
                     /locus_tag="ROI21_003440"
     CDS             14241..14660
                     /gene="trxC"
                     /locus_tag="ROI21_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDX5112322.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            14729..15427
                     /gene="tapT"
                     /locus_tag="ROI21_003441"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             14729..15427
                     /gene="tapT"
                     /locus_tag="ROI21_003441"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDX5112323.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            15459..18119
                     /gene="pat"
                     /locus_tag="ROI21_003442"
     CDS             15459..18119
                     /gene="pat"
                     /locus_tag="ROI21_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDX5112324.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            18233..19588
                     /gene="pssA"
                     /locus_tag="ROI21_003443"
     CDS             18233..19588
                     /gene="pssA"
                     /locus_tag="ROI21_003443"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDX5112325.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            19634..19957
                     /locus_tag="ROI21_003444"
     CDS             19634..19957
                     /locus_tag="ROI21_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDX5112326.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSATFGLMFSVGLGASKELWDSRPEGSGWSWKDFTWDVAGATTGYTVW
                     QLTRH"
     gene            complement(19954..21252)
                     /gene="kgtP"
                     /locus_tag="ROI21_003445"
     CDS             complement(19954..21252)
                     /gene="kgtP"
                     /locus_tag="ROI21_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDX5112327.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT         5234 a         5656 c         5387 g         5232 t
ORIGIN      
        1 agatgggcta ttaatggatg ccgccaatgc gacttaagcg cacacctgtc ggccattcgc
       61 cttcttgctt atcgaagctc atccagatag ccgttgcccg accgaccaga ttcgcttccg
      121 gcacaaagcc ccagtaacgg ctgtccgcgc tgttgtcgcg gttgtcaccc atcatgaagt
      181 attgtcccgg cggaacaatc caggttgcca gttgttgccc tggctgctgg taatacatcc
      241 ccacctggtc ctgcgcaatc ggcactgtca gaatgcggtg cgtcacatca cccagtgtct
      301 ctttacgctc ggaaagacga attccatttt ctttggtttc gtttttcggc acttcaaaga
      361 atccgctggt cgcttcccca ccattacggc gtgagaaggt ctgaacgaaa tcgctcggtt
      421 ccacgttgga gtaggtgacc ggcagcgcgt tttcacatgc ctgaccggaa ctgcatcccg
      481 gttgaatcgt cagctctttc gagaccggat cataagtgac tttatcgccc ggtaaaccca
      541 ccgcgcgctt gatgtaatca agctttggat cttccggata tttaaagacc acgatatcgc
      601 cgcgtttcgg atgaccggtt tcgatcagcg ttttctggta gataggatct ttaatgccat
      661 aagcaaactt ctctaccaga ataaaatcac cgattaacag agtcggcatc atcgaacctg
      721 acgggatctg gaacggttca taaataaacg aacgcacaat caatacgata gccagcaccg
      781 gaaaaacaga agcaccggtt tccagccagc caggcttcgg cgcaactttc ttcagcgttg
      841 ctttatccag tgagtcaccg gcagccgcct gcgccgctgc ctgacgttcc cgccgtttag
      901 gtgcgaaaaa gaatttatcc acgcaccata aaatgcccgt caccagtgtg gcaatcacca
      961 gaatcagggc aaacatattc gccatgccaa ctcctaaggg ttatttgttg tctttgccga
     1021 cgtgcagaat ggcgaggaac gcttcctgcg gcagctcgac gttaccgatc tgcttcatgc
     1081 gtttcttacc ttctttctgc ttctgcagca gctttttctt acggctgata tcgccgccat
     1141 aacatttagc cagtacgttt ttacgcagct gtttcacggt ggagcgcgca atgatgtgcg
     1201 taccaatcgc tgcctgaatg gcgatatcaa actgctggcg tgggatcaga tctttcatct
     1261 tctccaccag ctcgcgaccg cggttttgcg aattatcacg gtgggtgatc aacgccagcg
     1321 catcaacacg atcaccgttg attaatacgt ctacacgtac catgtcggac gcctggaagc
     1381 gtttgaagtt gtaatccaga gacgcataac cacgcgaggt agatttcagg cgatcgaaga
     1441 aatcgagcac cacttccgcc atcgggatct cgtacgtcag cgccacctga ttaccgtggt
     1501 aaaccatatt ggtctgcacg ccgcgttttt ctacgcacag tgtaataacg ttgccgagat
     1561 atgcctgcgg cagcagcatg tgacactctg caatcggctc gcgcagttcg tagatgttat
     1621 ttaccgctgg cagcttggat gggctgtcga cgtagataac ttctcttgac gtggtttcaa
     1681 cttcatacac tacggtcggc gcagtggtga tcagatccag atcgtattca cgttccagac
     1741 gttcctggat gatctccatg tgcagcaggc cgaggaagcc gcagcggaaa ccaaagccca
     1801 gcgcgctgga gctttccggc tcatagaaca gtgaagcatc gttcaggctg agtttgccca
     1861 gcgcgtcacg gaaggcttca tagtcgtcgg aacttaccgg gaacagaccg gcgtatacct
     1921 gcggtttgac tttcttaaag ccaggcagcg ccttttctgc cggattacgc gccagcgtta
     1981 aggtatcgcc tactggagcg ccgtggatat ctttaatcgc acataccagc caacctactt
     2041 cgccacattt cagttcagtg cggtcaacct gttttggcgt gaagataccc agacggtcgg
     2101 cgttataggt ctgcccggta ctcatgactt tcactttgtc gcccttacgc agggtgccgt
     2161 ttttaatacg gataagtgaa acaacgccca ggtagttgtc gaaccaggag tcgataatga
     2221 gtgcctgcaa cgggccttcc ggatcgcctt ccggcggcgg aatgtcgcgc accagacgtt
     2281 cgagaacgtc ctgcacgcca acgccggttt tcgctgaaca gcgcaccgcg tcggtggcgt
     2341 cgatgccgac aatatcttca atttcttccg ccacgcgttc aggatcggct gccggcaggt
     2401 caatcttgtt cagtaccggc accacttcga gatccatttc catggcggtg tagcagtttg
     2461 ccagggtttg cgcttctacg ccctgcccgg cgtcgaccac cagcaatgca ccttcacagg
     2521 cagccagcga acgagaaact tcataagaga agtcaacgtg gcccggggtg tcgataaagt
     2581 taagctgata ggtttcgccg tcagacgctt tgtagtccag cgtcacgctt tgcgctttga
     2641 tggtaatgcc acgctcacgc tcaagatcca tggaatcgag aacctgcgcc tccatttcac
     2701 ggtcagacag gccaccgcag atctggataa tacggtcaga cagcgtcgat ttaccgtggt
     2761 caatgtgagc tatgatcgaa aagttacgta tattcttcat aaagtatgat tattgtgcct
     2821 taatgcccgg ttaaccaggc ttttagaagt cgctgttctg agcttaacgt ctgtattaat
     2881 agaaacgccg cattctacac tacaacattg aggcgaggaa atgttcatac cgtatggatt
     2941 gtggtatctg gaaacgtcct cgcatttgtt atgcaaaatg caacaaagcc agtgaaatca
     3001 ctggctcgcg tcttccgaag atgtttcaaa tcgcacaagg ccaggcggca atgccacgct
     3061 taagatgatc ggttgccatt cagaccgggc agcaaacttt cgagagcagc cacgtgcaat
     3121 caggaatcca ccaacgccac cgagaatcgc accgcataat gctgcaatat ctgaagcaaa
     3181 gagtagctga aatagtgaag cgatgaggaa tagtcccatc aacggcgaca tataaaccag
     3241 taatgcggag ctaagcaggc tgccttcggc gattcctaat tctacttttt gccccggcac
     3301 taacggctca tcacagggta cgacaatggt atgcgtggtt tgcgggccaa gtttattcaa
     3361 cacgcggcta ccgcaaccgg cacgtgaagc gcagctgctg catgaggctt taacatcaca
     3421 actgaccagc gcctgcccgt tttcccagga gacgacggta gcccactctt tgatcattgc
     3481 gctgccccga acttaatatt ctcggcaatg cgtttcgccg tttgcggcgg cagttcaccg
     3541 acaatggtga tttcggcgtt atcacgtacg cttgtactga cggttctgcg tccggtgcgc
     3601 aacatctgat cggtgctcga tggcgtagcg cggttaacgt ttaccgaaaa gctgaataat
     3661 ccgtcggaat agagacgtga ttcgataggc atgttgtcca tcgtcggtag cggacgtcgg
     3721 ctactggaaa cttcgctaaa accctgtggc aaccaggttg gcgtccagct gaatttagct
     3781 ttttcaccta caggaacaga aagcaacggc ggcaaatttg ccttcgccag cgtctgcata
     3841 ctgctgctga tatcctgatt gacattaaaa gcaatcacgc gaaattgttc cagcgtttca
     3901 ccatcgcgat caaggagatc aaccctcatc ggtaatttcg attcggtgtc catccacacg
     3961 atgtagctgt agcgtgtacc atctcgggca accacgcgaa taacttcgca aagacgatca
     4021 gcaatacgcg tgcgcccgac ggagataaag tcgtagtaag gagaaaggcg tttgaaatcg
     4081 gtatagatga gcgatggcag agaatcaaca atgtaatcgc cattaagcgt gaacggttca
     4141 agtcccggtt caaaatagct gatttcattg ccgcgctgta ccacttcccg gcgcgggcca
     4201 tccatttgca acaattgtgc aagaggacgg ttatcgaggc gtgcatgtcg ataacgcaga
     4261 gactcaacac cctgtttatt gatgctgatg aatgacagct cgtaattcag tgactgactg
     4321 gccaggttca tctgctgtaa taacgccccg gacgcggggg tggccgaggc gttagcagag
     4381 aataacaggc tacctgtcac taatgacatg gcaaaccaaa gttgcttcat tactgcgatt
     4441 gcgttcctaa agtttgaatt cctggcacct gtacagcggc ttgctgggtt tgcgcctgct
     4501 caaactgaag ctgttcagag tggagtcggc gttgcagttc gtaatcctgc aacattgcat
     4561 taatgcgacg acgctgctcc tgtacctgct gctgttgacc attatttgcg gtcgcttcag
     4621 aaggtactcc caggctaacc gggctggctt tacccatcat cggcagtgta ttaaataccg
     4681 gcgtttcggg ctgctgggac gtttcagatt gtccattata gtgctggacg ccaacgataa
     4741 ctgcaagcga tacgcatgcg gctacgccca tttgggtaag ctgtgccgcc cacggacgta
     4801 ctttctgcca gaatggcatt ttctgccatt gatgcggcgc aggctgggct tccgggatca
     4861 atgtcgccgg ttgacgtact ggctcttctt caatggcggc catcacgcgt gaagagatat
     4921 cgaaatggag cacctcggga gtatcacccc gcattgagtc acggattaag tgatagcttt
     4981 cccaggtttt ctgcatttct gggttatgag ccagttcgtt aagcagctca ctatccagcg
     5041 tttcgccatc cattaaagcg gaaagttgtt ctttctgcat gcctaatacc cttatccagt
     5101 atcccgctat cgtcaacgcc tgataagcgg ttgaactttg ttatcaatag cttccctcgc
     5161 tcggaagata cgtgaacgca ccgtacctac cggacaatcc atgatagcgg ctatctcttc
     5221 atagctcagg ccatccagct cccgcaaggt tattgccatg cgtaaatctt ccgggaggga
     5281 ctcaatagtt cggaaaacta tctgtctcag ttcttctgac aacattaagt tctcagggtt
     5341 cgaaatttct ttcaacgcgc cgccactttc gaagttttca gcttcaatgg catccacatc
     5401 actggaaggt ggacgacgcc cctgagcaac caggtaattt ttcgctgtat ttacagcaat
     5461 ccgatacagc catgtataaa aagcgctatc tccccggaac gaatccagcg cacgataggc
     5521 tttaataaaa gcttcttgta ccacatcggg aacatcaccc gacggcacat agcgggaaac
     5581 cagactcgcc actttatgct gatagcgcac taccagtaag ttaaaggctt tctgatctcc
     5641 cttctggacc cgttcaacca ggacctggtc cgttaactgc tcgctcatcc gaggtaaagt
     5701 ctccccaaac caaatttcca cgcgctatcg aaacgccact ccattagctg caatttgagc
     5761 aagcaaaggg ttagagtgtc tcgtttttgt aaagttccgt aacgcatctg tttttgtttg
     5821 tcatgctgta gacggatcat tatctatcat tataagtcta cagaatctga acatcgcatt
     5881 atctgtgtag aaatgcccat ttaactgcct gaagagtaac ccaacggcct ttttatttca
     5941 ccacctaatc ctccaccagc cagtaacttc tctttttctc gccgccctgc gtcagcgtgt
     6001 ttagccactg taacaaatat taaaatagca ggtgtttaat cgcacaacat gatgctatgc
     6061 tgaccaaacc gtgtttagta aattaaacaa agaaaatgaa tactctccct gatcattcat
     6121 gtgacgtgtt gattatcggt agcggcgcag ccggactttc actggcgctg cgcctggctg
     6181 atcagcatca ggtcatcgtt ctaagtaaag gcccggtaac ggaaggttca acattttatg
     6241 cccagggcgg tattgccgcc gtgtttgatg aaactgacag cattgactcg catgtggaag
     6301 acacattgat tgccggggct ggtatttgcg atcgtcatgc agttgaattt gtcgccagca
     6361 atgcacgatc ctgtgtgcaa tggctaatcg accagggggt gttgtttgat acccacattc
     6421 agccaaatgg cgcagaaagc taccatctga cccgtgaagg tggacatagt caccgtcgta
     6481 ttcttcatgc cgccgacgcc accggtagag aagtagaaac cacgctggtg agcaaggcgc
     6541 tgaaccatcc gaatattcgc gtgctggagc gcagcaacgc ggttgatctg attatttctg
     6601 acaaaattgg cctgccgggc acgcgacggg ttgttggcgc gtgggtatgg aaccgaaata
     6661 aagaaacggt ggaaacctgc cacgcaaaag cagtggtact ggcaaccggc ggtgcgtcga
     6721 aggtttatca gtacaccacc aatccagata tttcttctgg cgatggcatt gctatggcgt
     6781 ggcgcgcagg ctgccgggtt gccaatctcg aatttaatca gttccaccct agcgcactgt
     6841 atcacccaca ggcacgcaat ttcctgttaa cagaagcact gcgcggcgaa ggcgcttatc
     6901 tcaagcgccc ggatggcacg cgttttatgc ccgatttcga tgagcgcggc gaactggccc
     6961 cgcgcgatat tgtcgcccgc gctattgatc atgaaatgaa acgcctcggt gcggattgta
     7021 tgtatctcga catcagccat aagcctgccg attttattcg ccagcatttc ccgatgatct
     7081 atgaaaaact gctcgggctg ggcattgatc tcacaaaaga accggtgccg attgtgcctg
     7141 ctgcacacta tacctgcggt ggtgtaatgg ttgatgatca tgggcgtacg gacgtcgagg
     7201 gcttgtatgc cattggcgag gtgagttata ccggcttaca cggcgctaac cgcatggcct
     7261 cgaattcatt gctggagtgt ctggtctatg gctggtcggc ggcagaagat atcaccagac
     7321 gtatgcctta tgcccacggc gtcagtacgt taccgccgtg ggatgaaagc cgcgttgaga
     7381 accctgacga acgggtagta attcagcata actggcacga gctacggctg tttatgtggg
     7441 attacgttgg cattgtgcgc acaacgaagc gcctggaacg cgccctgcgg cggataacca
     7501 tgctccaaca agaaatagac gaatattacg cccatttccg cgtctcaaat aatttgctgg
     7561 agctgcgtaa tctggtacag gttgccgagt tgattgttcg ctgtgcgatg atgcgcaagg
     7621 agagtcgggg gttgcatttc acgctggatt atccggaact actcacgcat tccggtccat
     7681 cgatcctttc ccccggcaat cattacataa acagataaaa agcctgggtc agcgccgtat
     7741 acgcttcgga atagttctgg tctggcccac gaatgactaa gcgatcgcta aagcattctc
     7801 ctgcctgcgg ggagaatgcc agcagcaccc gatgcggcag tcgcgcttcg ttttccgcca
     7861 catccgtccg caaacgtaaa tgccagccca tacttaatgc cagttcagta aaaccattac
     7921 caatctgctc tggcagcacc acgcagaaaa atccctcttc ggtaatgcac tccgccgcac
     7981 aggtcagcaa cgatgggtga tcaagcgtag tggtatagcg agcctgttcc cgttgaggtg
     8041 tcgagcactc tactccctgc tgatagtaag gtgggttact gatgattaaa tcgaagcgta
     8101 ctgtctgctg tgtgatccac tgctgaatat ccgccgtatg gacgttaatc cgctctgccc
     8161 acggggactg gttgatattt tcctgcgcct gcgcggcagc ttcactttcc agttcaactg
     8221 catcaatcat tacgctgtca tcggttcgct gcgccagcat taatgccagc aacccgctac
     8281 ccgcgccgat atcaaggcaa cgttttaccc cagccaccgg tgcccatgcg cccagcaaaa
     8341 tgccatccgt tccaactttc atcgcacaac gatcgtgagc aacaaaaaac tgtttaaaag
     8401 taaatccatt acgacgaagc acggatgtag actgtgacat gaaaataaaa ccttgcagga
     8461 aaaacggcga tagcaccggg tgagaacaat acccgagaag cgatatccat acaaacagat
     8521 gaagattgca gccgtaacgt ctataatcag cgccccacac agaggtagaa catgactgta
     8581 acgacttttt ccgaacttga actcgacgaa agcctgctgg aagccctcca ggataaaggt
     8641 ttcactcgcc cgaccgccat tcaggctgcc gccattccgc ctgcgctcga tggccgtgat
     8701 gtactcggtt ctgcgccgac aggcaccggt aaaacggcgg cgtatctgct gccagcgttg
     8761 cagcacctgc tcgatttccc acgtaagaaa tccggtccgc cgcgtatttt gatcctcacc
     8821 ccaactcgcg agctggcgat gcaggtagcc gatcatgccc gcgaactggc gaaacatacg
     8881 catctggata ttgccaccat caccggcggc gtagcctata tgaaccacgc ggaagtgttc
     8941 agcgaaaatc aggacatcgt ggtcgccacg accggacgtc tgctgcaata cataaaagaa
     9001 gagaacttcg attgccgcgc ggttgaaacg ctgatcctcg acgaagcaga ccgtatgctg
     9061 gatatgggct tcgctcagga tatcgaacat attgctggcg aaacgcgctg gcgtaaacag
     9121 accctgctct tttcggcaac tctggaaggc gatgcgattc aggactttgc cgagcgtctg
     9181 ctggaagatc cggtggaggt ttctgccaat ccgtccaccc gtgagcgcaa aaaaattcat
     9241 cagtggtatt accgcgccga tgacctggaa cataaaaccg cattgctggt gcatctgtta
     9301 aaacagccgg aagcgacccg ctcaattgtg tttgtgcgta agcgtgagcg tgtgcatgag
     9361 ctggcaaact ggctgcgcga agcgggcatc aacaactgct atctcgaagg tgagatggta
     9421 caaggcaagc gtaacgaagc gatcaagcgt ttgaccgaag gccgcgtaaa cgtactggtt
     9481 gccaccgatg ttgctgcacg cggtatcgac attcctgatg tcagccacgt ctttaacttc
     9541 gatatgccgc gcagtggtga tacttatttg caccgtatcg gacgtaccgc gcgcgccggt
     9601 cgtaaaggca ccgcaatttc gctggtggaa gcccatgacc atctgctgct gggcaaagtt
     9661 ggccgttata ttgaagagcc aattaaagct cgcgtaattg atgagttacg cccgaaaacg
     9721 cgtgcaccaa gcgagaagca gaccggcaag ccatcgaaga aagtactggc taaacgtgcc
     9781 gagaagaaaa aagctaaaga gaaagagaag ccgcgggtga aaaaacgcca tcgcgacacc
     9841 aaaaatattg gtaagcgccg taaaccaagc ggaacgggcg tgcctccgca aacgacagaa
     9901 gagtaatttc aatgccgggt ttaagcccgg cattattttt atcccggcaa aaaacactct
     9961 tcaacacact gaataaaagt gtgcatcgcc gggctaaccg cttttccagc atggtgtgcg
    10021 cacattgctg taatcgtctg cgactgttcg ccaaaaggca attcgattaa ctcgccgctc
    10081 gccaattctt ttgctaccgc aaagcgcggc aggtagctga ccccgatatt cgccgcgaca
    10141 caacgtttga tactttcaat gcttaacagc tcaatggtgt tttccaccgt gatccgccgc
    10201 tggcgcagcg tgctctcaaa tatctgccgg aatacacatt gcggttcgtt gataataaag
    10261 ctacaggcgt tatgtcttcc cggctcagta aaatcgacat ctgcaatttg cggtgaagct
    10321 accagcacca gcgattgctc acccaattct cgtcggttta gagcatcatc attccctacg
    10381 cggtaaaaga cgccgacatc agcctcatca ttcagtagcg catcacggat cacgtaacag
    10441 ttcagagact gcaacgataa acgcactttt ggtgcgcgtt gccgaaaacg ctgcaacacc
    10501 tgcggcattc ggtaagaaag caacgtttcg cctgaaacaa cgcgtagttc cccgtccgga
    10561 tccgactctt ttttggcagc ctcacgaagg gtatccatca ctctggtgag ttcataaatg
    10621 tgcggcagca gcttttttcc ttcgcgggta aggcacattc gtcgaccaat tttctcaaat
    10681 aactggactg agaactcctg ctcaagctgc tgaatatgaa aagtcaccgt cgattgtgtg
    10741 cagcacaatt tttgcgaagc tcgcaaaaag gaaccctctt ccaccacggt tttaagcgta
    10801 ataaaacggc gcagatccat aaccccaaac ctatcgaaaa tatcgaattt agaatgtaaa
    10861 aacattcatt tttttaaatg ttccgtgtcg ggtactgtct accaaaacag aggagataac
    10921 aagtgacacc gaccctttta agtgcttttt ggacttacac cctgattacc gccatgacgc
    10981 caggaccgaa caatattctc gcccttagct cagctacaac gcacggattt cgtcagagta
    11041 ctcgcgtgct ggcagggatg agtctgggat ttttaatcgt catgttgcta tgcgtgggaa
    11101 tttcgttttc actggctgtt atcgacccgg cagcagtgca tctgttgagc tgggcaggag
    11161 cggcatatat tgtctggctg gcatggaaaa tcgctaccag tccaacaaag gaagacggac
    11221 ttcaggcaaa accaatcagc ttttgggcca gctttgcttt gcagtttgtg aacgtcaaaa
    11281 tcattttgta tggcgttacg gcactgtcga cgtttgtcct gccgcaaacg caggcgctaa
    11341 gctgggtagt tggcgtcagc gttttgctgg cgatgattgg gacgtttggc aatgtgtgct
    11401 gggcgctggc ggggcatctg tttcagcgat tgtttcgcca gtatggtcgc ccgttaaata
    11461 tcgtgctggc gctgttgctg gtctattgtg cggtacgcat tttctattga aagacaaaaa
    11521 gaaaagcgga agaggtcacc ctcttccgct taataacttg ctacttaagc cttacaggct
    11581 ttcagtaaag gtacgagcga taacgtcgcg ctgctgttcc ggagtcagag agttaaagcg
    11641 aactgcataa ccggatacac ggatggtcag ctgcggatat ttttccggat gcttaactgc
    11701 atcttccaga gtttcacgac gcagaacgtt aacgttcagg tgttgaccac cttcaacgcg
    11761 aacttctggt ttcacttcta ctggaacttc acggtattca atatcaccca gtttgcttac
    11821 tgcaaccact tcgtcttctg caaaacctgc tttcgcaacg atgcaacgcg cttcgccttt
    11881 ttcgctgtcc agcagccaga aagagttcag cagatcgtcg ttagcggctt tagtaatctg
    11941 gatacctgta atcatgtgat gcctccccgg caaaattatt tgatttgttc agcctgtcgc
    12001 ggccaattgg taaaaccatt gttgcttgag tgtatatata ctcctcaaac acccttgaat
    12061 ctttgattta aatcaataaa aaccacacat caagtatggt tgcaaatgga ttttattgtt
    12121 ttacatcaac ttatgcaggt gtgaaatttt accaatttac atttttttgc actcgtttaa
    12181 gtctaaaaaa tgagcatgat tttgttctgt agaaagaagc agttaagcta ggcggattga
    12241 agattcgcgg gagagcgaga tggcaaacga attaacctgg catgacgtgc tggctgaaga
    12301 gaagcaacaa ccctattttc ttaataccct acagaccgtc gccagcgagc ggcagtccgg
    12361 cgtcactatc tatccgccac aaaaggatgt ctttaacgcg ttccgcttta cagagttggg
    12421 tgacgttaaa gtggtgattc tcggccagga tccttatcac ggaccgggac aggcgcatgg
    12481 tctggcattt tccgttcgcc ccggcattgc gacaccgccg tcactgctga atatgtataa
    12541 agagctggaa aatactattc ccggtttcac ccgccctaat catggttatc ttgaaagctg
    12601 ggcgcgtcag ggcgttctgc tactcaatac tgtgttgacg gttcgcgcag gtcaggcgca
    12661 ttcccacgcc agcctcggct gggagacctt caccgataaa gtcatcagcc tgattaacca
    12721 gcatcgcgaa ggcgtggtgt ttttgttgtg gggctcacat gcgcaaaaga aaggggcgat
    12781 tattgataag caacgccatc acgtactgaa agcgccgcat ccatcaccgc tttctgcgca
    12841 tcgtgggttc tttggctgca accattttgt gctggcaaat cagtggctgg aacaacgtgg
    12901 cgagaaaccg attgactgga tgccagtatt accggcagag agtgagtaaa tttgcaggaa
    12961 aacgccggat ggcagcgttg ccacccggct gatttatcag gctttattct gacgccacca
    13021 ttcaccaagc aaaacgccag ttgcgacaga gatatttagc ccggcaacgt tgccagtacc
    13081 gtcaatcttc acgcgcagat cgtttggatc gcgtgcggca tccggtaacc cttcatattc
    13141 ctgacccagc accagtacca ttttcgctgg cagactggtt ttgaacagcg gtttcccctg
    13201 ctcgctggaa gtggtcacta cggtgtaacc tgcctgacgg aaatcatcca gcacgttaac
    13261 aatgttgtcg ccagtaatcg gctgaacgtg ctctgcgccg ccttctgcgg tacggatagc
    13321 cgcccccgac tccagcagtg cctcatcctg caccacaacg cctttcacgc cgaagtgcgc
    13381 acagctgcgc atcatgccac ccaggttatg cgggttagat tcgttttcca gtgccagaac
    13441 gcaatcctgc gcgcctgcct ggcttaccca ctgctgcacg gttgtaccgt tacgcttttt
    13501 gatcaggaag caaacgccgc cgtgatgttc cgtgcctgac gcttttgtca gttccgcttc
    13561 atctaccaca tggtacgctt tgcggtttgc tgccatccag cgcaaggctt ctttaaaacg
    13621 cggcgttaca ctctggataa accaggcgcg aacaatcgct tccggacggc tctggaacag
    13681 tgcctgacag gcattttcgc cgtagacgcg ggtttcttcc gcacgctgac gacgcaaaac
    13741 ttccggatca ataaaacttt taccactgat gccaccgtga tcggcctttt ccggcgtctc
    13801 atcacccggc gcgcgggaaa ccgtgcgcca cggcgaatct tcccacttgc ggtcacgggg
    13861 gtgactctgt ttgtcatcgc gggcggggcg acggccaccg tcagcacgag attttcctgg
    13921 acgcccgccc cctttcccgg tacgcgggtt gtgggtacgt ttatcagaat catcatcact
    13981 gcggacatac atcactttga ccttgccgct tttacctttc atttcatcgt tcatgctttt
    14041 ctccaccagc gctgcgcgaa gcgcgcagat tacccgaagt ccgcgcggtt cgccatgatt
    14101 tcgtaccaaa gcctgcgact atcataccta ttgaataaaa cagattgttg tctggaacaa
    14161 tgtccccgat aatatgtaac atattagaaa cataccggcg tcgttgccga taagtctcct
    14221 tactcatccc gaggttagtt atgaataccg tttgtaccca ttgtcaggcc atcaatcgca
    14281 ttcccgacga tcggatcgaa gatgcggcaa aatgcggacg ctgcggtcac gacttgtttg
    14341 acggagaggt gattaatgcg accggtgaaa cgctcgacaa attgctgaaa gatgatctac
    14401 ctgtggtgat cgacttctgg gcaccgtggt gcggcccctg ccgtaatttc gcaccaattt
    14461 ttgaagatgt cgcgcaagag cgtagcggta aagtgcgctt tgtgaaagtg aataccgaag
    14521 ctgaacgtga attgagcagt cgctttggaa ttcgtagtat accgacgatc atgattttca
    14581 aaaacggtca ggttgtcgac atgcttaatg gcgcagtacc gaaagcgccg tttgatagct
    14641 ggctgaacga atctctttaa tcttaccggg gcgcatcttg tgccccgttt tctcctctgc
    14701 gacaatggcg ttttttcgac gctctcttat gaccgaaaac gctgttctcc agttacgcgc
    14761 cgagcgtatt gcgcgcgcaa cacgtccttt tcttgcccgc ggtaatcgcg ttcgtcgctg
    14821 ccaacgctgt cttttgccag agaaattatg tctctgttcg acaattaccc cagcacaagc
    14881 aaaaagtcgc ttctgtttgc tgatgttcga caccgagcca atgaagccca gtaataccgg
    14941 gcgtctgatt gctgatattt tgcctgatac cgttgcgttt caatggtcgc gtaccgaacc
    15001 ctcgctagat ttgctggatc tggtacaaaa cccggactat cagccaatgg tggtctttcc
    15061 cgcctcgtat gctgatgagc aacgggaagt gatcttcaca ccacctgccg gtaagcctcc
    15121 gctatttatc atgctcgatg gtacctggcc tgaagctcgc aagatgtttc gtaaaagtcc
    15181 gtatctggat aatcttcccg tcatttccgt cgatctttcc cggctttctg cctatcgcct
    15241 gcgtgaagcc caggctgaag gccaatattg tactgccgag gtagccatcg cactgttaga
    15301 tatggccggc gataccgggg cggcggcagg gttaggcgag cattttaccc gctttaaaac
    15361 acgctatctg gcaggaaaaa cgcaacatct gggtagcatc acagcagaac agttagaaag
    15421 cgtttaaaat cattcggtca cttctgcggg agaccggtat gagtcagcga ggactggaag
    15481 cactactgcg accaaaatcg attgcggtaa ttggcgcatc gatgaaaccc aatcgcgcag
    15541 gttacctgat gatgcgtaac ctgctggcgg gaggctttaa cggaccggta ctcccggtga
    15601 cgcctgcctg gaaagcggtg ttgggtgtgt tggcctggcc ggatattgcc agcttgccct
    15661 ttacacccga ccttgcggtt ttatgtacca atgccagccg taatcttgct cttctggaag
    15721 agctcggcga gaaaggctgt aaaacctgca ttattctttc cgccccggca tcgcaacacg
    15781 aagatctccg cgcctgcgcc ctgcgccata acatgcgcct gctaggacca aacagtctgg
    15841 gtttactggc tccctggcaa ggtctaaatg ccagcttttc acctgtgccg attaaacgcg
    15901 gcaaactggc gtttatttcg caatcggctg ccgtctccaa caccatcctc gactgggcgc
    15961 aacagcgtga gatgggcttt tcctacttta ttgcgctcgg cgacagcctg gatatcgacg
    16021 ttgatgaatt gctcgactat ctggcgcgcg acagtaaaac cagcgccatc ctgctctatc
    16081 tcgaacagtt aagcgacgcg cgacgctttg tttcggcggc ccgtagtgcc tctcgcaata
    16141 aaccgattct ggtgattaaa agcggacgta gcccggcggc tcaacgtctg ttgaacacca
    16201 cggcaggaat ggatccggcg tgggatgcgg ctattcagcg tgccggtttg ttgcgggtac
    16261 aggatactca cgagctgttt tcggcagtgg aaaccctcag ccatatgcgc ccactgcgtg
    16321 gcgaccggct gatgattatc agcaacggtg ctgcgcctgc cgcgctggcg ctggatgcct
    16381 tatggtcacg caatggcaag ctggcaacgc taagcgaaga aacctgccag aaactgcgcg
    16441 atgcactgcc agaacatgtg gcaatctcta acccgctcga tctacgcgat gacgccagca
    16501 gcgagcatta tgtcaaaacg ctggacattc tgctccacag ccaggatttt gatgcgctga
    16561 tggttattca ttcgcccagc gccgctgctc ccgcaacaga aagcgcgcaa gtattaattg
    16621 aagcggtaaa gcatcatccc cgcagcaagt atgtttctct gctgacgaac tggtgtggcg
    16681 aacattcctc gcaagaggca cgacgattat tcagcgaagc cgggctgccg acctaccgta
    16741 ccccggaagg aaccatcact gcttttatgc atatggtgga gtaccggcgt aatcagaagc
    16801 aactacgcga aacgccggcg ttgcccagca atctgacctc caataccgca gaagcgcatc
    16861 ttctgttgca acaggcgatt gccgaaggag caacgtcact cgatacccat gaagttcagc
    16921 ccatcctgca agcatatggc atgaacacgc tccccacctg gattgccagc gatagcaccg
    16981 aagcggtgca tattgccgaa cagattggtt atccggtggc gctgaaattg cgttcgccgg
    17041 atattccaca taaatcggaa gttcagggcg taatgctcta cctgcgtaca gctaatgaag
    17101 tccagcaagc ggcaaacgct attttcgatc gcgtaaaaat ggcctggccg caggcgcgga
    17161 tccacggcct gttggtgcaa agtatggcta accgtgccgg cgctcaggag ttgcgggttg
    17221 tggttgagca cgatccggtt ttcgggccgt tgatcatgct gggcgaaggc ggtgtggagt
    17281 ggcgtcctga agatcaagcc gtcgtcgcac taccgccgct gaacatgaac ctggcccgct
    17341 atctggttat tcaggggatc aaaagtaaaa agattcgtgc gcgcagtgcg ctacgcccat
    17401 tagatgttgc aggcttgagc cagcttctgg tgcaggtttc taacttgatt gtcgattgcc
    17461 cggaaattca gcgtctggat attcatcctt tgctggcttc tggcagtgaa tttaccgcgc
    17521 tggatgtcac gctggatatc gcgccgtttg aaggcgataa cgagagtcgg ctggcagtgc
    17581 gcccttatcc gcatcagctg gaagaatggg tagaattgaa aaacggtgaa cgctgcttgt
    17641 tccgcccgat tttgccagaa gatgagccac aacttcagca attcatttcg cgagtcacca
    17701 aagaagatct ttattaccgc tactttagcg agatcaacga atttacccat gaagatttag
    17761 ccaacatgac gcagatcgac tacgatcggg aaatggcatt tgtagcggta cgacgtattg
    17821 atcaaacgga agagatcctt ggcgtcacgc gtgcgatctc cgaccctgat aacatcgatg
    17881 ccgaatttgc cgtgctggtt cgctcggatc tcaaagggtt aggcttaggt cgacgcttaa
    17941 tggaaaagtt gattacctat acgcgagatc acggactgca acgtctgaat ggtattacga
    18001 tgccaaacaa tcgtggcatg gtggcgctgg cccgcaagct cgggtttaac gttgatatcc
    18061 agctcgaaga ggggatcgtt gggcttacgc taaatcttgc ccagcgcgag gaatcatgag
    18121 taaggtactg gaaatgttga ccacttaatc gggactggtg ttattattgc ccgcttatgt
    18181 cgtctgcatt gcacagagga cccttcaatg aacagagaag aaatgcactg tgatgttgtc
    18241 aaaatttaag cgtaataaac atcaacaaca ccttgcccaa ctacccaaga tttctcaatc
    18301 agttgatgat gtcgatttct tttacgctcc cgccgacttc cgggagacgc tgctggaaaa
    18361 aatagccagc gcgaagcagc gcatttgcat tgtcgccctg tatctcgaac aggatgacgg
    18421 tggcaaaggc attctgaacg cgttgtatga ggctaaaagg cagcgtccgg aactggatgt
    18481 gcgggtgctg gtcgactggc atcgtgcaca acgtggacgc attggcgctg cggcatctaa
    18541 cactaacgct gactggtact gccgcatggc gcaggaaaat ccgggcgtag atgttccggt
    18601 ttatggcgtt ccaatcaata cccgtgaagc ccttggtgtt ctgcacttta aaggctttat
    18661 catcgacgat agcgtacttt atagcggtgc cagcctgaac gatgtttacc tgcatcagca
    18721 cgataaatat cgctacgacc gttatcatct tatccgtaac cgtaagatgt cagacattat
    18781 gtttgaatgg gttacacaga atattatgaa tggccgcggc gttaatcgtc tggatgatgt
    18841 taatcggcca aaaagcccgg aaatcaagaa cgatattcgt ctgttccgcc aggagctgcg
    18901 tgatgccgct tatcatttcc agggcgatgc cgacaacgat cagctttccg taacgccgtt
    18961 ggtaggtctg gggaaatcga gtctgttgaa caagaccatt ttccatctta tgccttgtgc
    19021 cgagcagaaa ctaaccatct gcacacctta cttcaacctg ccagcaatcc ttgtgcgcaa
    19081 tattatccag ttgctgcgcg aagggaaaaa ggtcgaaatt attgttggtg ataaaaccgc
    19141 gaatgacttc tacattccgg aagatgaacc tttcaagata attggcgcat tgccttatct
    19201 ctatgagatc aatctgcgtc gtttcctgag ccgtttgcag tattacgtca acactgacca
    19261 gttagtggtt cggttatgga aagatgacga caacacctat cacctgaaag ggatgtgggt
    19321 tgatgataag tggatgttga tcactggtaa taacctgaac ccgcgcgcct ggcgtctgga
    19381 tctggaaaac gctattttga tccacgatcc gcaactggag cttgcgccac agcgagagaa
    19441 agaactggag ctgatccgcg agcataccac catcgttaag cactatcgcg acctgcaaag
    19501 tattgccgat tatccggtga aggttcgtaa actcatccgt cgtttgcgcc gtatccgcat
    19561 cgaccgatta attagccgca tcctgtaatc acaaccccgt cctgtacggg gtttgttttt
    19621 tggaggccac gttttgcgta ttctttttgt ctgttcgcta ttattacttt ctggatgcag
    19681 ccatatggct aacgatagct ggagcgggca ggataaagct caacacttta tcgcctcggc
    19741 gatgctttcc gccgccggaa atgaatatgc acagcatcag gggatgagtc gcgatcgcag
    19801 tgccactttc gggctgatgt tctctgttgg gctgggggcg tcaaaagaac tttgggatag
    19861 ccgccccgag gggagcggct ggagctggaa agattttacc tgggatgtcg ccggtgcaac
    19921 tactggctat accgtctggc aactgacccg tcactaaaga cgcatcccct tccctttgcg
    19981 atgtagcatc aaagaaacca gaaacgccac cacggccatc aaggtcacat accagaagaa
    20041 ggctgtttcc attcctattg atttcagcga caacgctacg tactccgccg aaccaccaaa
    20101 tatagcattg gcgaccgcat atgacagacc aacacctaat gcacgaacct gtgccgggaa
    20161 catctcagcc ttcagtattc cactgattga agtataaaaa ctcacgatca gcagggcaca
    20221 catcaccaga ccaaaagcgg cataaggcga ggaaacgttt tgcaatgctg agagaatagg
    20281 aacggtaaaa atggctgcca gagaaccgaa acataacatt gaggtacgac gaccaatctt
    20341 atccgacagc gcgccaatga gtggttgaat aagcatgaat acaaacaatg cggcagtcat
    20401 aatgccactc gccacgttgg catgcattcc cgcagtattt accagatact tctgcatata
    20461 agtggtgaag gtatagaaac aaagggagcc cgcagcggta aaaccgagaa ccatgatgaa
    20521 tgcacggcga ttgcgccata atcctttcag agatccagct tcttttaaag cgcgtgtttc
    20581 ttgttgtgaa gtttcatcta actgacgacg taaccacaac gccacaacag ctaacacagc
    20641 tcctaacgcg aaaggaatac gccatcccca ctctctgagt gcagcgtctt ccatggtgtg
    20701 ttgtaaaacc acgacaacca gtagagctag cagttgcccg ccgatcaacg tcacatactg
    20761 aaatgatgcg taaaaacctt tgcgcccttc aacggcaact tcactcatat aggtggcgct
    20821 ggtgccatat tctccgccaa cggataatcc ctggaataaa cgagcaagaa gcaataatgc
    20881 cggagcccag gtaccgattg tttcgtagcc tggcaaacag gcgataacca gtgagccgaa
    20941 acacatcata cacaccgata acagcatcga ttttttgcga ccatgtttat cggctatgcg
    21001 gccaaatagc caaccgccta ttgggcgcat cagaaatccc gcagcaaaaa cacctgctgt
    21061 ttgcagtagt tgagtcgtcg tgttcccgga agggaagaag atgtgagcga agtagagtga
    21121 acagaacgag tagacataga aatcgaacca ctcgaccaga ttacctgaag aggcccccac
    21181 aattgcccaa atgcggcgac gagtatcact acttgtcagt ttgctgtctg ccgttacagt
    21241 actttcagcc atgccattat gtctcctgcc gtaatccgat gcttttgtcg gtcgcttttg
    21301 tttatttctt ttggtaaaga aaatgttata catttgttgc atatcatcat gcaaccttaa
    21361 ccacgaattt agttagcagg aaaatggtta ttgaggagct taaggataaa tttctggtaa
    21421 ggaggacacg tatggaggtg ggcaagttgg ggaagccgta ttcgctggcg tatgtggagg
    21481 aataagctgc cccctatcgt gcctgcgta
//
LOCUS       DAPOZO010000084        21456 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPOZO010000084 DAPOZO010000000
VERSION     DAPOZO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.084"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1417)
                     /gene="dhaM"
                     /locus_tag="ROI21_003446"
     CDS             complement(<1..1417)
                     /gene="dhaM"
                     /locus_tag="ROI21_003446"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDX5112328.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMGDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADTDHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVHPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            complement(1428..2060)
                     /gene="dhaL"
                     /locus_tag="ROI21_003447"
     CDS             complement(1428..2060)
                     /gene="dhaL"
                     /locus_tag="ROI21_003447"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDX5112329.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLSVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMKMLALAAKE"
     gene            complement(2071..3141)
                     /gene="dhaK"
                     /locus_tag="ROI21_003448"
     CDS             complement(2071..3141)
                     /gene="dhaK"
                     /locus_tag="ROI21_003448"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDX5112330.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLILHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVAITVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            3369..5288
                     /gene="dhaR"
                     /locus_tag="ROI21_003449"
     CDS             3369..5288
                     /gene="dhaR"
                     /locus_tag="ROI21_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDX5112331.1"
                     /translation="MSSAFNNDGRDISPLIATSWERCIKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQEDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLHSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(5388..8273)
                     /locus_tag="ROI21_003450"
     CDS             complement(5388..8273)
                     /locus_tag="ROI21_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin family autotransporter"
                     /protein_id="HDX5112332.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKYGAIGLNAIIFSPLL
                     IAADLGSQNGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIVIQGKNSSLTANRLTVDVVSQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGSAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSSGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HTSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPAPAPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVL
                     DSQNNGVNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLQGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(9117..10208)
                     /gene="ychF"
                     /locus_tag="ROI21_003451"
     CDS             complement(9117..10208)
                     /gene="ychF"
                     /locus_tag="ROI21_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDX5112333.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(10325..10909)
                     /gene="pth"
                     /locus_tag="ROI21_003452"
     CDS             complement(10325..10909)
                     /gene="pth"
                     /locus_tag="ROI21_003452"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDX5112334.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            11187..11465
                     /gene="ychH"
                     /locus_tag="ROI21_003453"
     CDS             11187..11465
                     /gene="ychH"
                     /locus_tag="ROI21_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDX5112335.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(11520..13199)
                     /gene="dauA"
                     /locus_tag="ROI21_003454"
     CDS             complement(11520..13199)
                     /gene="dauA"
                     /locus_tag="ROI21_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDX5112336.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLMSGVFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(13324..14271)
                     /gene="prs"
                     /locus_tag="ROI21_003455"
     CDS             complement(13324..14271)
                     /gene="prs"
                     /locus_tag="ROI21_003455"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDX5112337.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(14422..15273)
                     /gene="ispE"
                     /locus_tag="ROI21_003456"
     CDS             complement(14422..15273)
                     /gene="ispE"
                     /locus_tag="ROI21_003456"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDX5112338.1"
                     /translation="MRTQWPSPAKLNLFLYITCQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEAHQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(15273..15896)
                     /gene="lolB"
                     /locus_tag="ROI21_003457"
     CDS             complement(15273..15896)
                     /gene="lolB"
                     /locus_tag="ROI21_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDX5112339.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            16110..17366
                     /gene="hemA"
                     /locus_tag="ROI21_003458"
     CDS             16110..17366
                     /gene="hemA"
                     /locus_tag="ROI21_003458"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDX5112340.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            17408..18490
                     /gene="prfA"
                     /locus_tag="ROI21_003459"
     CDS             17408..18490
                     /gene="prfA"
                     /locus_tag="ROI21_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDX5112341.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            18490..19323
                     /gene="prmC"
                     /locus_tag="ROI21_003460"
     CDS             18490..19323
                     /gene="prmC"
                     /locus_tag="ROI21_003460"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDX5112342.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQNLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            19320..19712
                     /locus_tag="ROI21_003461"
     CDS             19320..19712
                     /locus_tag="ROI21_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDX5112343.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIAFDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            19716..20525
                     /locus_tag="ROI21_003462"
     CDS             19716..20525
                     /locus_tag="ROI21_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDX5112344.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            20561..21415
                     /gene="kdsA"
                     /locus_tag="ROI21_003463"
     CDS             20561..21415
                     /gene="kdsA"
                     /locus_tag="ROI21_003463"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDX5112345.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         5271 a         5354 c         5491 g         5340 t
ORIGIN      
        1 aaccctgacg gctgaaacgt tgcgttttaa cgtccagcgt tagcgtttct tctggttgta
       61 tcgcatacag tttctcaccc tgctggcaaa tccagccaat ccccagttca cgggcgatca
      121 gggcgctgtg ggatagcgga cttccggcgc taaggcagat accttttaca accgccggat
      181 ccagttgcag tattgtggag ggatagatgt tctccgccag tagaatagtt ggcgagttaa
      241 actgcgggag ttcttctttc gtttgggtca ggtggaccag ggtgcgatgc agaagatcgt
      301 ccacatcaat atagcgagcc tgtagatatt catcatccag ttgctggtat tgctggctaa
      361 gttctttaag aacttgctgc caggcatatt ctgccgtgca atgttcgtgc tgaaggagtt
      421 cgcttgccgc cgccagcagt tccggatcat ctaacagtgt atggtgacca gaaaagattg
      481 cggcaatatc gtcaagcccg ctggcttctg ctttcgctgt taacgtcatc agatctaata
      541 acgtgaagtc aatggcctgg cgtaatcgtt cttgttcttc atccacggtc agggttgatt
      601 ttgcctgtac cgtacataaa accggttgat aataaaaggc tttacccgaa acaggcggaa
      661 cgggacgcag aataggtgga gcaacttcct ccgtttcacc aaagttatct tcagccagct
      721 gacggaaagc gatcagtgcc tcttcagctt ctggcccttt cgcaatcagg cgcagcgtat
      781 cgttataacg aacttgtagt aacgcaatct ggttaatact ctccggtgtg acgcatttgc
      841 cgtttttctc cagcaacata tcggcattaa atgtcgataa tgtataaacc agccgggagg
      901 ccggatgtac atgcaggccg ttacggtttt ttatgaccac cgccagagaa cgggcttctt
      961 catcgtacgg aggacatgtg tcagagattt cagtgtcgga ggagggtaaa cccagttgtt
     1021 cacgtttggc ttccagcgca tgcatggcgt caaagataac tttgtcgatc tccgcccccg
     1081 aggccgcgct gaccgttgct gccagtgtac cttcgaccaa cggtgcagca cacaaacgta
     1141 cttttgcggc gatctcggga gccagcaatt ccagcgcagt ttcagcactc aataatgcgc
     1201 tacccatatc catcatgacc agcacatggt cggtatcggc aacagattcg atggcctcca
     1261 tcactttgac ggcatcggta ccaatgggat tttgtggatc gtcaattccc gcggcaatgg
     1321 cgattttaca actatcaccc attaacatct gacgggctaa ttcaccgaca ccttctccca
     1381 gtcggctgct atgtgaaact atgaccaggt ttaccatcac caattcctta ctcttttgcg
     1441 gctaacgcca acattttcat cataaacatc accgaggtcg cgccgggatc ctggtgacca
     1501 atactgcgtt caccgagata actggcgcgg cctttgcggg cttgcatcgt aatcgtactt
     1561 tgtgcagcgg attcggcgat gctactggca gcgtcgagcg ccgccggaac agagagattt
     1621 tgctcgcagg actgacgtaa cgattccacc accggcaccc acacatcaca catggtttta
     1681 tcgccaggtt cggctttccc gcgactgatt acgccgtccg cgccatcgcg gaacatctga
     1741 taaagctctt ccagcgtcag gctttgccgt gcctgggtcg cctgtgcggc gcggataaag
     1801 aaggtaccga acagcggacc actggcacct ccgacgctgg aaagtagcgt catgccggta
     1861 ttcttgagaa tgaaaccgat atctttatct gcgatagcag ggagtttttc caccactttg
     1921 ctaaagcctc gattcatatt aagcccgtgg tcagcgtcgc caatctcgcg atccagtccg
     1981 gtaagatact cgctctcggt gctgaaaata tcgccacaac gagtgagcca gttaacaatt
     2041 tgagttctgc tcagtgacat tgctttctcc ttatttaccc cagttaaggg ccggggtgtg
     2101 gaccggggcg tcccagagtg ccagcgtttc gtcatcaact ttcagtaagg tgattgagaa
     2161 accggtcata tccagtgagg tgcagtacgc gccaattaaa ttacgttcga tagtcaatcc
     2221 tgcttgctgg caacgtgtgg tcaggcggtt atagacgccg tatagctcag aaagcggagt
     2281 tgcgccaaga ttgttaacca gcgcaatcac ccgatcgcca gactggagcg gttgtttggt
     2341 ttgtggttct tcctgccagc tgccttgttg ataatcccag aaacgcaaag tgcgatggta
     2401 tgagccattt tccagcaggg tgtcgaacat ttcatcgacg gtttgatcaa gggaagagaa
     2461 gggacggcgg tcaatacctg gctcaccatg aatgccgacg ccaaactcca tctcattgtc
     2521 cgccagggta aaagaaggtt tgcccgcggc aggaacggta caggcaccga gagcaatacc
     2581 tattgagtgg ccttgattat tcaacttacg ccccagttcc gcacaggcgt ccagtgagtc
     2641 gccacgctcc gccgctgcgc ctacgagttt ttcaattaat acggtgatgg caacgccgcg
     2701 ccgcccggcg gtataaagac tgtcttttac tgcaacgtcg tcatcaatga ccacagtggt
     2761 cacttttacg ccgctatcgt gcagtaactc ggttgctgtt tcgaagttaa gaatatcgcc
     2821 ggtgtaattt ttgataatca acagtacacc ttcgccgcca tcaatttgca tggcgcattc
     2881 aaagatttta tcgggcgtcg gtgaggtgaa aatttcgccc ggacaggccc ccgaaagcat
     2941 cccctgaccg atataaccac aatgcatcgg ctcgtgtccg ctgccgccac ccgacagcag
     3001 ggcgacttta cctgcaacgg gggcatcagc tcgggtgaca tacaccggat cctggtgcag
     3061 tatcagcgat ggatgcgctt tcgctagtcc tgccagttgt tcgtccagta cgtcttgcac
     3121 atcattgatc aattttttca ttattttgct ccagcaatta cggtagggca tggatgatgt
     3181 tcaacgacac ggcgaccact gactgccgat gaatccattg tgcatcaggc aagggaaaag
     3241 aaaattagcc caaattatgt ttcatagtga aacatattct ttaatgaatg ttccatattg
     3301 aaacttttac gtgtattaat acttaaaatt gcgagccgga acaccttttg tcataaggga
     3361 tgcgggatat gagtagcgct tttaacaacg atggtcggga catatctccc ttaattgcaa
     3421 cctcctggga gcgatgcatt aagctgatga aacgggagac atggaacgta ccacaccagg
     3481 cccagggcgt gacatttgcg tctatttatc ggcgtaagaa agcgatgctg acgctcgggc
     3541 aggctgcgct ggaagatgcc tgggaatata tggcaccgcg agagtgtgcg ctgtttatcc
     3601 tcgatgaaac cgcctgcatt ctcagccgta atggcgatcc gcaaaccttg cagcagctaa
     3661 gtgcactggg attcaatgac ggcacgtatt gcgccgaggg aattattggt acttgtgcgc
     3721 tatcgttagc ggctatctct ggtcaggccg tgaaaacgat ggccgatcaa catttcaaac
     3781 aggcactctg gaactgggcc ttttgtgcaa cgccgttgtt tgacagcaag ggccgattga
     3841 cgggaacaat agcgctggcg tgtccggttg agcaaactac cgcagctgat ttgccgttga
     3901 cgttggcaat cgcccgagag gtcggtaatt tactgctgac ggacagtttg ctcgctgaaa
     3961 ctaaccgtca tttaaatcaa cttaatgccc tgttagaaag tatggatgat ggcgtgatta
     4021 gctgggacga gcagggtaat ttgcaattta tcaacgccca ggcggcgcgg gtcttgcgcc
     4081 ttgacgcgac ggcaagtcag ggaagggcaa tcactgaact cttaacgtta cccgccgtat
     4141 tgcaacaagc aataaaacag gcacatccgc tcaaacacgt agaagcaacc tttgaaagtc
     4201 agcatcagtt tattgatgcg gtgataaccc ttaaaccgat aatagaaacg cagggaacca
     4261 gctttatttt gttgctccat cctgtggaac agatgcggca gttaatgacc agtcaattag
     4321 gaaaagtcag ccatactttc gctcatatgc cacaagagga tccgcaaacc cgccgcttga
     4381 ttcattttgg tcgccaggcg gcgcgcagta gctttcctgt cctgctttgt ggagaggagg
     4441 gcgtgggcaa ggcactgcta agtcaggcaa ttcataatga aagcgagcgt gcagcggggc
     4501 cttatattgc cgtcaattgt gagttatatg gtgatgcggc gctggcggaa gaatttattg
     4561 gtggcgatcg cacggataat gaaaatggcc gtctgagtcg gctggaactg gcgcacggcg
     4621 gcacgctgtt tcttgaaaag attgaatatc tggcggtgga gttacagtct gctttgcttc
     4681 aggttatcaa gcagggtgtt atcacgcgac tggatgcgcg gcgtttaata cccattgatg
     4741 tcaaagtgat tgccacaacg accgcggacc tcgcaatgct ggtggaacaa aatcgcttta
     4801 gtcgccagct gtattacgcg ctgcatgcat ttgaaattac catcccgcct ctgcgtatgc
     4861 ggcgtggcag cattccggcg ctggtgaata acaaattaca cagtcttgaa aaacgcttct
     4921 ctacgcggct gaaaattgat gacgatgccc tcgctcgcct ggtttcctgt gcatggcctg
     4981 gcaacgattt tgaactttac agcgtcatcg agaatcttgc tctgagtagt gataacgggc
     5041 gcattcgcgt cagtgattta cctgaacatc tgtttaccga gcaggcgaca gatgatgtca
     5101 gcgccacccg cctttccacc agtctgtcat ttgcggaagt tgaaaaagag gcaattatta
     5161 acgcagccca ggtcacaggc ggtcgcattc aggaaatgtc ggctttactt ggaatcggcc
     5221 gcactacgct gtggcggaaa atgaagcaac atggcattga tgcagggcag tttaagcgcc
     5281 gggtatgaaa gacagaaacg atttatgata catcagagtg atctgtattt cattccggcg
     5341 cacgctaaca attttcagca tcgtttaagg gcttgtctat cccccactta aaagctgaag
     5401 cgatatcctc cgttgacttg cttctgatca aagaggttac cctgcgtgta atccgcttcg
     5461 agatagaatg tgtgctgttt gttatactgt gcactgactc ccacgccgtt attccagcca
     5521 ttacctttga aactgtactt ctcccgggag ttgttcaaca gatattcggt atcccctgaa
     5581 aactcgcgga tagcgccagt cttcacatag acattcagct ggctgttgcc tgcggtgatg
     5641 tcatatccca gtatcatgct ggcacgcccc tgcagcgatt cgtagtgatt cagatgtata
     5701 ttgaggccat tactcgcctt catagccatc tcattctggt ggctgtatgt gagctgggtt
     5761 tgcggctcta tatagaaccc ataaccagta ggggacaggt tgaacctctg cccggcttcc
     5821 agggagatac tcattccatt cgcagtgccg ttggcgttaa cgccgttgtt ctgactgtcc
     5881 agtacgtgga aactattttt ctggcgcgat gcttttataa cgagatcgct gtaaaaaccg
     5941 ttttgtgcca tgtaactggc gtacattccc atgtagtctg aacgtgcggt accgtcgcct
     6001 ccgctatagt ccggcgatgc atgtgttgag ccaatataca gaccgacata caacggcatt
     6061 acatcagaga gacgtttatc cccaccaaac tggataccgc tgtaacccat gtcaaagccg
     6121 ctcagtttgc cactggcgaa ggagtccaga cttcccccat aactgcgcag ccagatattt
     6181 ccgtctttac tctgatttcg caggtcaccc atccgttgca tcagcgtacg gttttcaaca
     6241 tagttcaata agtaaccgac attgagataa ttgccgccag catctgcggt cgtggtgggt
     6301 ttaggtgtgg gagcgggggc aggcgtagga tcgggttcag gcgtaggatc ggggttgact
     6361 atgggaggct gagcgggagc tggtgtgggt tcaggattag gagtcggttc cggaactgtc
     6421 ccggaagcgt aaagctccca gttagtgcca tttttacgca catcgtacag atatcccccc
     6481 aactcaacct gagaagacgc gctgaacgag gccgcgccgt cagtggtttt taccactgtc
     6541 agaacttcat ttcccgttgt ggcctcgctg ccctggttac ggatagccaa tacgtgatta
     6601 ccagcactac tcccgctgat attcaataaa tcccctttat tattaacgcc attaccctcg
     6661 ccaacaacat cagcacgcat aataaaggta ctgttaccgc tcaggttctc tacgtttaat
     6721 gtggtaaatg tgccggcagt tgacgtgtgg ctggcaaaat cgacagttga atggctcagc
     6781 gccagcgtgt cgagattaga attacttgtt acgttccaga cgctgttatt cattgcaacg
     6841 tccagtttcc cgccattgac attatcgctg agggaagaac ctgtccaaac cgacccaggg
     6901 tgcatatcca gattgattag cccacctttg gaaagaacgc taccgttgat aagcatacga
     6961 cccgaggcgt tgatgcggct ggcggcataa ccatcgtcat gttgcgttgc aatggccatc
     7021 tggtcgggtg tactcatatc aatgaccaga tcgctcgtga gatcgatctg gctgttggtc
     7081 atggcataaa tccccctggc tcctgccgcg ccggtgatag ccaaactgtc tccggttatt
     7141 ctaccgccgc tgagcgccca caaacccagc gccagactgc cattacgatc aaccgtaata
     7201 tcggtatttt gcatgttgac aacagccgtt gccgtctggg ctgaggcacc ataagaaccg
     7261 ctggaaaaga tgctgtttcg ttgcgctgcc gtgccagaaa aattgattgt cgcgtctgaa
     7321 ccactggtta cgaggccacc gccctgcgcg gaagaaatat ggctatcagc accgatagtg
     7381 gttgtaccac cacgaacttc gacgccattg gccgcggatc cagttacatc aacagtgagt
     7441 gcattcgcgc tcacctggcc aaagctccag aggccgtgcg cattatcgcc attggtttta
     7501 atggtactgt ttgttccgag atcgacaaca gagtttttct gtacgtttat tcccatggcg
     7561 ctgtagccct gaacatcgat tgtcaggtct gtcgccgtaa aacgcgcagc accattggcg
     7621 ttattgccgt tgagaccacc gatataaaca cctgtacttc catcggtctt gattttactt
     7681 ccgcttccaa gatcgacact ggtgccatag tcattgatgg ttaggcctat accgttcgag
     7741 ttttcaatgg tgaattgagt ggctgttagt gttgagctat gtccaataat gatgccgtca
     7801 tcgttactct taatggtgct gcctgtgcct aagtcagcat gggtatagtc accaattaaa
     7861 ttaatgccga tggcagaggt ttgactaaca acatctactg taagtcggtt agctgtcagg
     7921 gagctgttct tgccctgaat aacgattccc tttgcatatc cagaggcgtc gttgacattg
     7981 acggtgacat cattacccag attgatattg ccaggcgtgt ttcctgctgg ggtcattaca
     8041 ccatagaggt ttcctgatgg atcggcggta tctcctgtaa ttctgtcacc atcattaata
     8101 gtaatattgg tgccattttg acttcctaaa tcagcagcta tcagcagggg ggaaaagata
     8161 attgcattca aaccaatagc gccatatttt atcagttgaa tggagggcga acggattttt
     8221 aattcagcga gacgatcggc tttggtattc ccattgtgtt gtttgatgcc cataccactc
     8281 ctatatagta cccaggctat gggtctatgg attttgtcat tttgtttaag tcaatgaata
     8341 atatcctctt atcatcatga tatttgtatg gtgcaatcat gatgagctgc tctgagaata
     8401 attctctatt accgggcaaa atcaggaaca caggaattaa tatatattta acatccccct
     8461 tacaaaggtg agtttagatc gagttattcc ctaagttgtc gttacttatt ccaattcgtt
     8521 acaatgaata tgacaaaaac attacaacaa acagcggtta aataatttgg tgaatatttt
     8581 tggtaataat cctaattggt tatgggattt tcgcttgatg tatctgcata acaaaaacaa
     8641 tatgagccat ttttgcatta ctaataagaa aaagcaaaaa acatcgatat tcatttttga
     8701 tcaatggatt ttctggcata tcgttattat cgtgatactg gttatcattt tcatcttgcc
     8761 aggatcgcac gtaatcacgt tattcgggga ttttatctgc gggatattac ccttcaggta
     8821 agtccttttc tcgccagcat attggagcgt gatcaatatt gatcagctgt gaagggggct
     8881 gatgtgtagt ggaacaaaaa attacgttat atcactgcta atgagatatc cgtcatctga
     8941 acatttatgt ctttaattat tctcgtggtt cactgtaggc aataagcaca aaagtgtagg
     9001 atgttataag aatggttagg actcggtgaa atgaaaaatc cacgcaattg cgtggatttt
     9061 atatactttt gcgctcttca tgagatttag cgaaacctca tgagacaaca aattaattag
     9121 acgttgaaca ggaagttcat tacatcgcca tctttaacga tgtaatcttt gccttctgca
     9181 cgcattttgc ctgcttcttt cgcgccttgt tcacctttgt aagtgatgaa atcttcaaac
     9241 gagatggttt gtgcacggat aaagcctttt tcaaaatcag tatggatttt gccagcggcc
     9301 tgcggcgcgg ttgcaccaac cggaatggtc catgcacgta cttctttcac cccagcggtg
     9361 aagtaagttt gcaggttcag cagcttataa ccggcacgga tcacacggtt caggcccggc
     9421 tcttccagcc caagctcctg cataaactcg tcacgttctt cgtcgtccag ttcggcaatg
     9481 tctgcttcaa cagcagcaca aaccggaacc acaacagaac cttctttcgc cgcgatttca
     9541 cgcacctggt caagatacgg gttgttttca aaaccgtctt cgttgacgtt ggcgatgtac
     9601 attgttggtt tcagcgtcag gaagctcagg taacgaatag ccgctttctc ttcagcgctt
     9661 aaatccagcg cgcgcagcat acctgcgttt tccagctggg gcaggcattt ttccaggacc
     9721 gccagctcag ctttcgcgtc tttatcgcca cctttggctt tcttctgtac gcgatgaatc
     9781 gcacgttcgc aggtgtcgag gtccgccagc gccagttcgg tgttgataac ttcaatatca
     9841 tcagccgggt taactttgcc ggaaacgtga atgatgttgt cattttcaaa gcagcgaaca
     9901 acgtgaccga tcgcttcagt ttcacggatg ttggtcagga actggttacc cagaccttcg
     9961 cctttcgatg cgccttttac cagaccggcg atatcgacaa attccatggt cgtgggaagc
    10021 gtacgctgcg gttttacgat ttcagccagt tgatccaggc gagggtcagg cattggtacg
    10081 acgcctgtgt tcggctcgat ggtgcagaat ggaaagttgg ccgcttcaat accggctttg
    10141 gtcagcgcgt tgaacagggt agatttcccg acgttgggca aaccgacgat accgcatttg
    10201 aatcccatga tttaaatcac cttaatatct taataatcaa cctgttattg ctaacagatt
    10261 gcagaaatgg aaataacttt gcctattata cacggcactc ggcaaaaatg ccgcagacaa
    10321 cgacttattg tgctttaaag gcgtgcaatc ggttcgttgc cttggtcaag ccatctgtaa
    10381 accacatttc agtacaacgc gccgcttcgt cgatggcttc atcaattaac ttctgttcac
    10441 taacaggcgg tttgcctaat acaaaaccaa caactttatt tttatcgccc ggatgaccga
    10501 ttccgatgcg taaacggtga aagttagggt tattacccaa tttactgatg atgtctttca
    10561 gtccattgtg accaccatgg ccaccgccca acttaaattt ggcgacgcca ggaggcagat
    10621 ccagttcgtc gtgggccacc agaatttcgt ccggattaat gcggaaaaaa ctggccatcg
    10681 ccgcaacggc tttgccgctg agattcataa atgtagtcgg gactaacagg cggacatctt
    10741 cgcctccaag ggtgactcgc gaagtataac caaagaattt agcctcttcg cgcagcggag
    10801 cgcgcaatcg ctctgccagt aagtcaacga accaggcacc agcattatgt cgcgttgcgg
    10861 cgtattcagc gccggggttc gccaggccga caatcaattt aatcgtcacg tttttttgtc
    10921 ctgagtgtgt acataactgg cgcgtagttt actggttgcg gccccgcttg acaaaaaact
    10981 gcgtatcaaa tgcagataac gcaataattg cctgagtgga ctattagaaa gtcaaggtgt
    11041 tcaggcattt atttgtaaag ttttgttgaa ataagggttg taattgtgat cacgcccgca
    11101 cataacccac agggtgttgt ctatacttta cacataagga agaggggtat tccctgttac
    11161 aacccagaaa gttccggagg tgacatatga aacgcaaaaa cgcttcgtta ctcggtaacg
    11221 tgctcatggg gttgggtctg gtggtaatgg tggtcggcgt ggggtattca atcctcaacc
    11281 agttaccaca gtttaatatg ccccagtatt tcgcacatgg tgcagtgcta agtattttcg
    11341 tcggtgccat tctctggctg gcgggtgccc gtgttggcgg gcatgaacag gtgtgcgacc
    11401 gttactggtg ggttcgccac tatgacaaac gttgccgccg tagcgataat cgccgtcata
    11461 gctaacaaat aatgcagttt gctgaccagt cagtttgcgc tgactggtca atctcgtact
    11521 tataaatccg ccatcgccgc gcgacgattc gggaagaaag caaggcggcc cgggatcggt
    11581 tgaatgcctg cgcgagccat agtgcgcagt ggctggaatt ccaggttgca cacgcgcagt
    11641 tcacatcctt cgggcagacg cttcacaaaa cgctggaacg catcaagacc accagcatca
    11701 agtaccggaa cggcatccca cttcagaatc acaatccgtt tgccttcaag acgtgactcc
    11761 aggtccgtga acaagccttc agcggcagca aaaaacagcg gaccaataac gcgcagaacc
    11821 agaacatcgt ctggaacgtc tacgactacc ggtgccaggc gagtcatacg tgcgatacga
    11881 cgcataaaca gtagcgatgc cagcacgatc cccacgctga tggcaataac catatcaaac
    11941 agcacagtca gcgacatgca cagcagcatg acgatgatgt catctttcgg cgcatgacgc
    12001 agcaagtcga ccactttgtg cgcttcactc atgttccacg ccaccatcaa cagcagggca
    12061 gccatggcgg aaagcggcag ccaggagagc agcggtgcca gtaccagcag ggcaagaata
    12121 accagaatag agtggatcac cgccgagata ggggaagttg ccccggcacg gacgttagcg
    12181 gcagaacgcg cgatggcagc tgtagcggta ataccaccaa agaacggggc gatgatattc
    12241 cccagtccct gtccaaccag ttcgctgttc gctttgtgtt tcgttccggt cataccatcc
    12301 agtaccacgg cgcagagcag agattcgatt gcgccgagca ttgccattga gaatgctgca
    12361 ggcagcagtg tgcgaataga atcccaggtg agggtaaatt ctgagttggg caggtcccac
    12421 ggtagtacca gttgcggcag cagttgcgga ataccgttac cctgagaacc atctgccaga
    12481 acgtagtgga attgcgatcc gatggtagca acatgcccgc cgagcaggtt aacaatcccc
    12541 atcaccgcgc aaccagccag caaggccgga aggtgaccag gcaaacgaat gcccagacgc
    12601 ggccagaaaa ccagaatgcc aagcgtcaca atgccaatgg cagcatcacc cacattaatg
    12661 gtcggcaacg ccataaataa tgcgccgact ttttgtagat aatgctccgg gacatgggcc
    12721 atttgcagac cgagaaaatc tttaatctgc atggtaccga tggtgatccc gatacccgag
    12781 gtgaaaccta aggtcacgga aaccggaata tattcaatca agcgtccaaa acgtgccagc
    12841 cccatcagga tcaaaaagac acctgacatg agtgtcgcga ccagcagtcc tgccagtcca
    12901 aactgttgcg aaacgggata gagaattacc acaaatgccg cagtcggacc ggaaacgcta
    12961 aagcgtgacc cccccgtcag agcaatgaca atccccgcaa cagctgcggt atataaaccg
    13021 tactggggtg ccacaccact accaatagcc aacgccattg ccagcgggat agcaataatc
    13081 ccgacggtta tcccggcaat caggtcacgg gtaaaccgtg cggcagtata tttttctttc
    13141 cagcaagcgt cgatcagagc gcggaaaggc atcacatgtg aggaaaatat tttgttcaca
    13201 ataatgtttc atccgtgagc gcatcatctg tcaactaaat ggcaggtgaa ggaggcatag
    13261 gtcatacaaa tggatattac agacaaaaaa acccgccgca gcgggtcttt gagccgggtt
    13321 cgattagtgt tcgaacatgg cagagatcga ttcttcgttg ctgatacgac gaatcgcttc
    13381 ggccagcata cctgacaggg tcagagtacg cacgttcggc agtgatttga tttcatcgct
    13441 cagcggaatg gtatcgcaga caacgacttc atcaattaca gagttacgca ggttgttcgc
    13501 cgcgttgcca gagaagatcg ggtgagtcgc gtacgcaaat acacgtttag caccacgttc
    13561 tttcagagct tcagcagctt tacacagcgt accgccagta tcgatcatat catcgaccag
    13621 tacgcagtca cgacctgcaa cgtcaccgat gatatgcatc acctgggaaa cgttcgcacg
    13681 cggacgacgt ttgtcgatga ttgccatatc ggtatcgttc agcagcttag cgatagcgcg
    13741 ggcacgcaca acgccgccga tgtccggaga aaccacaata gggttatcca gattcagctg
    13801 cagcatgtct tccagcagga tcgggctacc aaatacgtta tcaaccggaa cgtcgaagaa
    13861 accctgaatc tgttcagcgt gcagatccac tgtcagcaca cggtcaacac cgacgctgga
    13921 gaggaagtct gcaaccactt tcgcagtgat tggtacacga gcggaacgga cgcgacggtc
    13981 ctggcgcgca tagccaaagt aggggataac agcggtgata cgacctgcgg aagcacgacg
    14041 cagggcatca accataacga ctaattccat caggttgtcg ttagtagggg cacaagtgga
    14101 ctggatgatg aaaatatcac caccgcgtac attttcgtta atttgtacgc tgacttcgcc
    14161 gtcgctaaag cgacctacag cggcgtcgcc gagtgaagtg tacaggcggt tggcaatacg
    14221 ttgtgctagt tccggggtgg cgttaccagc aaaaagcttc atatcaggca cgagaagaac
    14281 ctcaggcatg cgtccattgg tggaaagaat ctgccgaaaa ctgtgcgggc caggcatgat
    14341 tctttccagg cggtgtatta aagagcgcga tgcaacgtct ggaacaaggt gacgttgtca
    14401 ccgaaactca gcttgcccgg cttaaagcat ggctctgtgc aatggggaaa gattaacgcc
    14461 tttcgccaca aagccattga gccattccgg ggcttgctct agcacctggt gggcttcaga
    14521 ctctgtatca aattcagcaa agacacaggc ccctgtccca gtcaggcgcg acggggcgta
    14581 ttctaacagc caggaaagca ccgcatcaac ctcgcgaaaa cgttttcttg cgataacctc
    14641 gcaatcattg ctgaattcac attttagcaa cgtttctatt gacctttttg gcgtattgcg
    14701 cgggagttca ggatctttaa aaatcaccgg tgtcggaata cttacaccag ggtgcgccac
    14761 cagataccac ttctctggcg gatccaccgg cgttagtatt tcaccaacgc cttcggcaaa
    14821 cgcggcatgc ccccgaacaa agacaggaac atctgcgccc agcgtcagcc ccatttccgc
    14881 cagctcatcc atgcttagcc cgcattgcca gagatgattt aatgccacca ggaccgtcgc
    14941 ggcattggat gaaccaccgc cgagaccgcc gcccatcggc aaacgcttgt caatgctgat
    15001 atccgcaccg cttcccgtcg gaagacgccc gctgtctgcc gcagttttca tcaacaatcg
    15061 tgctgcgcga acgatcaggt tatcttcatg ttccacgcct tcaacgggcg ttaacagacg
    15121 aatatcccca tcgtcacgaa gctcaatgct gatggtgtcg ccgtaatcaa gaaactgaaa
    15181 cagcgtttgc agcgtgtggt aaccatccgc acgctgacag gtaatgtata aaaacagatt
    15241 aagttttgcc ggagagggcc actgtgtccg cattatttca ctatccagtt atccattttt
    15301 aacttgatgc gttgaccacc gtcggtgagt tccatattgg ctggcatcgc aggttgagtt
    15361 tttgtgtcat aaccaccgta aacaactttc cagtttttgc cattctggct gtaggtgatt
    15421 tcgctcaggc ggtactggtc gtccagtttg tagtcggttg catcacccgg taaacctaaa
    15481 atccactggc gcaagctgtt gagcggaatt ggcattccgg tcaatttgcc aatcatctct
    15541 tcggcgtcat cggcggtata acgctggcct ttattgtcga ctaactgcac gttacccggt
    15601 tgagcattca gctccagttc cgtgctgccc aatgggttag tcagcagcag acggtagcga
    15661 tcctgaccag tttgctgcca gaagaaacgg gcgtacactt tttgttggtc agaaatataa
    15721 gcgaacgcgc cgcgagtttg atactgatta agattgcgca cgtcttgctg atgctgacgc
    15781 cattgtggcg aatccgggct tttgccagga cctttggggg tggtaacgga acaggcagtg
    15841 agcacaagag cagccagcgg tagcaggcgg ataagacgaa aatcgggcag gggcatagtg
    15901 atgacaagtc cttgagatac gttacagtta taacccttaa tgctagcgtt accgcccgct
    15961 atcgtctatg ttcaagttgt cttaattgcc agaatctaac ggctttcggc aattactcca
    16021 aaagggggcg ctctctttta ttgatcttac gcatcctgta tgatgcaagc agactaaccc
    16081 tatcaacgtt ggtattattt cccgcagaca tgaccctttt agcgctcggt attaaccata
    16141 aaacggcacc tgtatcgctg cgagaacgtg tatcgttttc gccggataag ctcgatcagg
    16201 cgcttgacag cctgcttgcg cagccgatgg tgcagggcgg cgtggtgctg tcgacgtgca
    16261 atcgcacgga actttatctt agcgttgaag agcaggataa cctgcaagag gcgttaatcc
    16321 gctggctatg cgattatcat aatcttaatg aagaagatct gcgtaaaagc ctctactggc
    16381 atcaggataa cgacgcggtt agccatttaa tgcgtgttgc cagcggcctg gattcactgg
    16441 ttctggggga gccgcagatc ctcggtcagg taaaaaaagc ctttgctgat tcgcaaaaag
    16501 gtcatatgaa ggccagcgaa ctggaacgca tgttccagaa atctttctct gtagcgaaac
    16561 gcgttcgcac tgaaacagat atcggtgcca gtgctgtgtc tgtcgctttt gcggcttgta
    16621 cgctggcgcg gcagatcttt gaatcgctct ctacggtgac ggtactgctg gtgggtgcgg
    16681 gcgaaaccat cgagctggtg gcgcgtcatc tgcgcgaaca taaagtacag aagatgatta
    16741 tcgccaaccg cactcgcgaa cgtgcccaaa ttctggcaga tgaagtcggc gcggaagtta
    16801 ttgccctgag tgatatcgac gaacgtctgc gcgaagccga tatcatcatc agttccaccg
    16861 ccagcccgtt accgattatc gggaaaggca tggtggagcg cgcattaaaa agccgtcgca
    16921 accaaccgat gctgctggtg gatattgccg ttccgcgcga tgttgagccg gaagttggca
    16981 aactggcgaa tgcttatctt tatagcgtgg acgatctgca aagcatcatt tcgcacaacc
    17041 tggcgcagcg taaagccgca gcggttgagg cggaaactat tgtcgctcag gaaaccagcg
    17101 aatttatggc gtggctgcgt gcacaaagcg ccagcgaaac cattcgcgag tatcgcagcc
    17161 aggcagagca agttcgcgat gagttaaccg ccaaagcgtt agcggccctt gagcagggcg
    17221 gcgacgcaca agccattatg caggatctgg catggaaact gactaaccgc ttgatccatg
    17281 cgccaacgaa atcacttcaa caggccgccc gtgacgggga taacgaacgc ctgaatattc
    17341 tgcgcgacag cctcgggctg gagtagcagt acatcatttt ctttttttac agggtgcatt
    17401 tacgcctatg aagccttcta tcgttgccaa actggaagcc ctgcatgaac gccatgaaga
    17461 agttcaggcg ttgctgggtg acgcgcaaac tatcgccgac caggaacgtt ttcgcgcatt
    17521 atcacgcgaa tatgcgcagt taagtgatgt ttcgcgctgt tttaccgact ggcaacaggt
    17581 tcaggaagat atcgaaaccg cacagatgat gctcgacgat cctgaaatgc gtgagatggc
    17641 gcaggatgaa ctgcgcgaag ctaaagaaaa aagcgagcaa ctggaacagc aattacaggt
    17701 tctgttacta ccaaaagatc ctgatgacga acgtaacgcc ttcctcgaag tccgtgccgg
    17761 aaccggcggc gacgaagcgg cgctcttcgc tggcgatctg ttccgtatgt acagccgtta
    17821 cgccgaagcc cgccgctggc gtgtagaaat catgagcgcc agcgagggtg aacatggtgg
    17881 ttataaagag atcatcgcca aaattagcgg tgatggtgta tatggtcgtc tgaaatttga
    17941 atccggcggt catcgcgtgc agcgtgttcc tgctacggaa tcgcagggtc gtattcatac
    18001 ttctgcttgt accgttgcgg taatgccaga actgcctgac gcagaactgc cggacatcaa
    18061 cccagcagat ttgcgcattg atactttccg ttcgtcaggg gcaggtggtc agcacgttaa
    18121 caccaccgat tcggcaattc gtattaccca cttgccgacc gggattgttg ttgaatgtca
    18181 ggacgaacgt tcacagcata aaaacaaagc taaagcactt tctgtgcttg gtgctcgcat
    18241 ccacgctgca gaaatggcaa aacggcaaca ggccgaagcg tctacccgtc gtaacctgct
    18301 ggggagtggc gatcgcagcg accgtaaccg tacttacaat ttcccgcagg ggcgcgttac
    18361 cgatcaccgc atcaacctga cgctctaccg tctggatgaa gtgatggaag gtaagctgga
    18421 tatgctgatt gaaccgatta tccaggaaca tcaggccgac caactggcgg cgttgtccga
    18481 gcaggaataa tggaatatca atactggtta cgtgaagcaa taagccaact tcaggcgagc
    18541 gaaagcccgc ggcgtgatgc tgaaatcctg ctggagcatg ttaccggcag agggcgtact
    18601 tttattctcg cctttggtga aacgcagctg actgacgaac aatgtcagca acttgatgcg
    18661 ctactgacac gtcgtcgcga tggtgaaccc attgctcatt taaccggggt gcgagaattc
    18721 tggtcgttgc cgttatttgt ttcgccagcg accttaattc cgcgcccgga tacggagtgt
    18781 ctggtggagc aggcactggc gcggttgcct gaacaacctt gccgtattct cgatctcggg
    18841 acgggtaccg gggcgattgc gctggcgctg gctagcgagc gcccggactg cgaaattacc
    18901 gctgtagatc gtatgcccga tgctgtctct ctggcgcaac gtaatgccca gaatctggcg
    18961 atcaaaaaca tccatattct gcaaagcgac tggtttagcg cgctagccgg gcagcagttt
    19021 gcgatgattg tcagtaatcc gccgtatatt gacgagcagg acccacatct tcaacaaggc
    19081 gatgttcgct ttgagccgct cactgcgctg gttgcggcag acagtggaat ggcagacatc
    19141 gtgcatatca tcgaacagtc gcgtaacgcg ctggtatccg gcggctttct gcttctggaa
    19201 catggttggc agcagggcga agcggtgcga caggcattta tcctcgcggg gtatcatgac
    19261 gtcgaaacct gtcgggacta tggtgataac gagcgcgtaa cgctcggccg ctattatcaa
    19321 tgacaagttt ttctacactg cttagcgttc atctcattag cattgcgctt tctgtagggc
    19381 tattaacctt acgtttctgg ctacgttatc agaagcatcc acaggcattt gcgcgctgga
    19441 cgcgcattgt gccgccggtt gtcgatacgg tgttattgtt aagcggcatt gcgttgatgg
    19501 ctaaagcgca catcctgcca ttttccgggc aggcacagtg gctgactgaa aagctgtttg
    19561 gagttatcat ttatatcgtt ttgggtttta ttgcattcga ttatcgtcgt atgcacagtc
    19621 agcaggcgcg cattattgcc ttcccgctgg cgttggtggt gctgtacatc atcattaaac
    19681 tcgccaccac aaaagtaccg ttactggggt aagtcatgag atcgttagct gatttcgaat
    19741 ttaataaagc gccattgtgc gaaggcatga tcctggcttg cgaagcaatc cgccgcgatt
    19801 ttccctcgca agatgtttac gacgaactgg agcgtctcgt tagtctggcg aaggaagaaa
    19861 tcagccagct tctgccttta gaagagcagt tggaaaaact gatcgcgctg ttttacggcg
    19921 actggggatt taaagcctca cgcggcgttt atcgcctttc cgatgcatta tggctggacc
    19981 aggtgttaaa gaatcgacag ggcagtgcgg tatcattagg tgccgtttta ttatgggtcg
    20041 cgaatcgtct cgatttgccg ctattgccgg tgattttccc gacgcagctg atattgcgca
    20101 ttgaatgtcc ggatggcgaa atttggctga ttaatccttt taacggtgaa tcgttaagcg
    20161 aacatatgct ggatgtatgg ttaaagggaa atatcagccc gtcggcggaa ctgttttatg
    20221 aagaccttga tgaagctgat aacattgagg taatccgcaa attgctggat acactcaaag
    20281 cttcgttgat ggaagaaaat cagatggagc tggcgttacg caccagcgaa gctttattac
    20341 aattcaaccc ggaagatccc tatgaaattc gcgatcgcgg gttgatttat gcgcaactgg
    20401 attgcgaaca cgttgcgttg aacgatttaa gttatttcgt tgaacagtgt ccggaagacc
    20461 cgatcagcga aatgatccgt gcgcaaataa ataacatcgc gcataaacat attgtgctgc
    20521 attaattaat cgacatttta ctcaagatta aggcgatcct atgaaacaaa aagtggttag
    20581 cattggcgac atcaacgtag caaatgacct gccgttcgta ctgtttggcg gtatgaacgt
    20641 gctagaatct cgcgatctgg cgatgcgcat ttgcgagcac tacgtaaccg tgacccagaa
    20701 actgggtatc ccttacgtgt tcaaagcctc ttttgacaaa gccaaccgct cctccatcca
    20761 ctcttatcgt ggaccgggcc tggaagaagg gatgaaaatc ttccaggagt tgaagcagac
    20821 ttttggcgtg aaaattatca ccgacgttca cgaaccaagc caggctcagc ccgttgctga
    20881 tgttgtggat gtgattcagt tgccggcgtt tcttgctcgc cagactgacc tggttgaagc
    20941 catggcgaaa accggtgcgg taattaacgt caagaaaccg cagtttgtca gcccgggaca
    21001 gatgggtaat atcgttgata aattcaaaga aggcggcaac gaaaaagtga ttctttgcga
    21061 tcgcggtgct aacttcggct atgacaacct ggttgttgat atgctgggct tcagcatcat
    21121 gaagaaagtg tctggtaatt caccggtgat tttcgacgtg acccacgcac tgcaatgccg
    21181 cgatccgttt ggcgcagctt ccggcggtcg tcgtgctcag gtggctgagc tggcacgtgc
    21241 cggtatggcg gtaggtctgg cggggctgtt tattgaagcg catccggatc cggaacatgc
    21301 gaaatgtgat ggtccatccg cgctgccgct ggctaaactg gaaccgttcc tcaagcagat
    21361 gaaagcgatt gatgatctgg tgaaaggttt cgaagaactg gataccagca agtaatcttt
    21421 tttgcttgaa aactaaagcc ttagcgttct gcgtta
//
LOCUS       DAPOZO010000085        21161 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPOZO010000085 DAPOZO010000000
VERSION     DAPOZO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.085"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(124..1308)
                     /gene="araJ"
                     /locus_tag="ROI21_003464"
     CDS             complement(124..1308)
                     /gene="araJ"
                     /locus_tag="ROI21_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDX5112346.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(1434..4577)
                     /gene="sbcC"
                     /locus_tag="ROI21_003465"
     CDS             complement(1434..4577)
                     /gene="sbcC"
                     /locus_tag="ROI21_003465"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDX5112347.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEYDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRTLRQRLVALHGQIVPQQKRLAQLQVTIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRIQQLTPILETLPQSDELPHSEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAEALAQHQQHRPDGLTLTVTVKQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            complement(4574..5776)
                     /gene="sbcD"
                     /locus_tag="ROI21_003466"
     CDS             complement(4574..5776)
                     /gene="sbcD"
                     /locus_tag="ROI21_003466"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDX5112348.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQYLFTTTLH
                     TLAGEHEV"
     gene            5966..6655
                     /gene="phoB"
                     /locus_tag="ROI21_003467"
     CDS             5966..6655
                     /gene="phoB"
                     /locus_tag="ROI21_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDX5112349.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6713..8008
                     /gene="phoR"
                     /locus_tag="ROI21_003468"
     CDS             6713..8008
                     /gene="phoR"
                     /locus_tag="ROI21_003468"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDX5112350.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQIQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            8415..9734
                     /gene="brnQ"
                     /locus_tag="ROI21_003469"
     CDS             8415..9734
                     /gene="brnQ"
                     /locus_tag="ROI21_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDX5112351.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            9810..11183
                     /gene="proY"
                     /locus_tag="ROI21_003470"
     CDS             9810..11183
                     /gene="proY"
                     /locus_tag="ROI21_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDX5112352.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            11339..13156
                     /gene="malZ"
                     /locus_tag="ROI21_003471"
     CDS             11339..13156
                     /gene="malZ"
                     /locus_tag="ROI21_003471"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDX5112353.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EETSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            13342..14799
                     /locus_tag="ROI21_003472"
     CDS             13342..14799
                     /locus_tag="ROI21_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112354.1"
                     /translation="MTISNSNLLKPFITGSELPPFKEDVFSKTEEQFKKSPHGKLLFI
                     SGNENLVNISAEKIDEIKNSKDFFSNIVNIIILLIKHPDKILPILHGCPAESIELIKN
                     AKRTGQWSYEMTDKNGQSIEVSYIFTDTIDNNYDGKLCLKICNPCNQNDSSTHEYKID
                     HQQLKNHIRQLEEWALLEKFYDNNAYYSKRTKQLEKKIADAGFIIGKVNADNFNVKEF
                     KDDIASMLCEQPPMRTHQISSSGYLSADKVPDLYKTNLKDAQRDLKTTPVDIDGVSLK
                     PKTREKIAEKIAKKTEEENAKKIAKQNAKTIEEKNSEKNKKETNPEDNFVLKFSPEEI
                     NEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQGLYNVARNGSPRYAGLLRQIASKCA
                     ILQDFDQQLFWRTFSGGVFHRRISVNSEPEKTDVWRFSTQFDFPYINENGELSRTVLM
                     FEGVAESNFKDIANHSSECFSYSCYAAPLDANAKK"
     gene            complement(14820..15401)
                     /gene="acpH"
                     /locus_tag="ROI21_003473"
     CDS             complement(14820..15401)
                     /gene="acpH"
                     /locus_tag="ROI21_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDX5112355.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            15620..16690
                     /gene="queA"
                     /locus_tag="ROI21_003474"
     CDS             15620..16690
                     /gene="queA"
                     /locus_tag="ROI21_003474"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDX5112356.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKSAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            16746..17873
                     /gene="tgt"
                     /locus_tag="ROI21_003475"
     CDS             16746..17873
                     /gene="tgt"
                     /locus_tag="ROI21_003475"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDX5112357.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            17896..18228
                     /gene="yajC"
                     /locus_tag="ROI21_003476"
     CDS             17896..18228
                     /gene="yajC"
                     /locus_tag="ROI21_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDX5112358.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            18256..20103
                     /gene="secD"
                     /locus_tag="ROI21_003477"
     CDS             18256..20103
                     /gene="secD"
                     /locus_tag="ROI21_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDX5112359.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            20114..21085
                     /gene="secF"
                     /locus_tag="ROI21_003478"
     CDS             20114..21085
                     /gene="secF"
                     /locus_tag="ROI21_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDX5112360.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
BASE COUNT         4908 a         5259 c         5652 g         5342 t
ORIGIN      
        1 caacatttgg ggtgcagttc atgacgcgtc ttatcaggcc tacaaaatgt gccagaaccg
       61 cgtagggcgg ataaggcgtt cacgccgcat ccggcaataa gcaacgcgca ccgtgacagt
      121 aatctacccc agtggtttcg ccagcaccgg actatccgcc gcttgctggc gcttatagcg
      181 accatacagc agcaacgacg acatcgcagc aaacgaaagc agggcggcag gcagcgccac
      241 gtaattatat gccagcccca gcgtcagcat cataccaccg caatatgcgc cgacggcgct
      301 accgaggtta aacgctattt gcccacctgc ggcacctaat aactctccgc ctttggcatt
      361 ttgcagtaac aatatttgca gtggtgctga aagggcaaat aatcccgcgc aacaaataaa
      421 tgcaaaaata agcgacgttg ttttcatgcc gccgaagaaa aagagcatca gcagtgccag
      481 cacaataata aagtcagtta ctgccgcaat gcgcagtggt gaataacgtc ctgaaatccg
      541 gccacttagc acattcccca gcaccatccc tagcccaact aacatcataa taaaagtcat
      601 cgccgtttcc gaaaaaccgg aaataaacat catgtatggc tttacgtagc tgaaccaggc
      661 aaacacacct gcgttgccaa acatggtggc ggcgaaaatt aaccacgggg ccgggctgcg
      721 caaaaagtga aattgttcgc gtagcttccc tttcgcctcg tcgcgaatat ccggcaccca
      781 aaaatagacc gatgccatca ccgcaatatt aaaaacagcg atcaataaaa aggtgtaacg
      841 ccagctaaat tcctgactta aatacgttcc cagcggaatg cccagcaaat tggcgactgt
      901 catcccggaa accatccccg ccacggcggc ggtgactttg ccgggtttga taattttcga
      961 taacacgatc gcgccgacgc caaaaaatgc accatgcgga aagccggaaa ccagccgacc
     1021 aatggcgagc atcaggtaag acgaagagag cgtgaacatg gcgttgccaa tgacgcacaa
     1081 cgccaccaga aacaataaga tatgtttgag tgagtagcgg ctggaaaaga gtgcgatgat
     1141 tggcgcaccg accaccaccc ccagcgcata atacgagatc atatgcccgg cagcaggaat
     1201 cgaaattcct acgttatgcg ccagctccgt gagcacgccc ataatgccaa attcggccat
     1261 ccccaaacca aacgtgccca gagccaaaga taaaatgact tttttcatac caccatcaca
     1321 accaccagaa cggctaccaa tcaccgtagc caataaagtg atagcttgca ggataacgac
     1381 cagcaaggcc aagagataat tcgcccctct gtattcatta tcctgctgaa tagttatttc
     1441 actgcaaacg cactttccag tttgctgtag cccaggccgt tgatcttctt cactttgatc
     1501 tgcaccggaa tacgctcttt catcgcttca acgtggctaa tcacaccgat ggttttgcca
     1561 ctggcattca gggcatccag cgcatcaagg gcggtatcca gcgtttcgct atccagcgtg
     1621 ccaaaacctt catcaaggaa cagcgagtca atacgcgttt tatggctgac cagatccgaa
     1681 agcgccagcg ccagcgccag actgacgagg aaactttcgc cgccggaaag ggtacgggta
     1741 tcgcgtaccg catctgcctg ccaggtatca acaacctcga cttccagcgc ctcgctggct
     1801 ttgcgctgta acagatagcg cccgtgcagc cgggtaagtt gctgattagc gagatggact
     1861 aaattatcca gcgtcagccc ctgggcaaac ttgcggaatt tatcgccctc tttggaacct
     1921 attagcgaat tcagatatcc ccagtcctca acctgctgcg tcatttgagc aatttgctgc
     1981 attaaggttt gctgttgctg acggttgtct gcatcctgct tcagctgctg gcgaatctcg
     2041 ccctgactgg tggtgttttc acgcaacttt tggtgagttt gcgctaactc ttgctgaatc
     2101 tgcttcaccg tcacagtgag agtcaacccg tcaggtcggt gttgctgatg ctgtgccagc
     2161 gcttctgctg tctgagtgac cagcgtttgc gcctgacggc gctggttttc cagattctgc
     2221 ttgagctgtt ccagctgcgt tagtgtttgt tcatccatta gcgccgcaag gaacgcctgc
     2281 tgatcgtcaa agacgctggc ctgtagcgcg gtgtcaaact gcgcctgggc tttttgcaga
     2341 ctttgcgccg ccagaacgtc ctgttgctgt aacgtctgct gctggctgtg taatgcgaga
     2401 cattgttcat gaacctgccg ccagttttcc agcaccacag tttcttcgct gtgcgggagt
     2461 tcatcacttt gcggcaacgt ttccagaatc ggcgtcagtt gctgaatacg gttttgcagc
     2521 gcggttaatt cgttctggcg atgctgccag ctctgcgctt cttgctgacg cgtcgccaac
     2581 cagctctctt cttcatcttc ctgtggcaat gtcagtgcat aacccgccaa tgccgttaag
     2641 agctgctgct ggcgttgttc aatttgctgt tgatactgaa taatttgctg attatgcgcg
     2701 gcaatctgcc cttgtaattc atgccgttgg ctgagtaacc gcagctggcg ttcgtgctca
     2761 tcttgtgcat ccagccacgg ttgaatatcg tcctgtggct gcaaggtgat attgaggctg
     2821 gccgtgacgg cttgccattg ttgagtaagt gcttgctcat cttgtcggag gctttgcgct
     2881 tcgttttcat cgcgctgaag ctgcttcgtt aatgcatcca gttgcccacg tagcgccgca
     2941 ccttcttcgc cgagcttttt cacttctttt tccagcgtta atagccgggc ctgattaacg
     3001 cccggttcca gcgcctgata cgcctcgacc gccgggtggc tggtggaacc acaaagtgga
     3061 caaggctgac ccgcctgtaa ctgcgcgcgc tgggcttcca gcgttttgat gcgcgcttcc
     3121 tgctcgcaaa tggttttgac atcggcaagt tgctgcgtct tttctttata acgctggcgc
     3181 atttcgttaa gtgcggcgtt acgttgcgtc tgttcttgcg tgacattctg gatagtgacc
     3241 tgtaattgcg ccaggcgttt ttgttgggga acaatctgtc catgcaacgc gaccaggcgc
     3301 tgacgcagcg tgcgttgctc agcatgttgc gccagggcgg tagcaacttc atcggcggtt
     3361 aacgtcaacg tgatcgccgc aagcgcatta agtttttgct cagcatgggt taactgttgc
     3421 tgccattgcc gcagatgctc gcgatcgctg gtttgttggg agaactgcgc acgccaaccc
     3481 gccagttcgt tgttccactg acggaagcgg tcgtattcct gtaaccaggt attcaggctt
     3541 tgttgctgct gctgtaattc tgctgactgc ttcgccgcgt ggtggcgaat gctcgcgcga
     3601 agcgccattg tgctctgtaa gcgagtattt acttcttcaa tctgctggcg agtatgcgcc
     3661 agcgccgcgc tgtgttctgc gatgcgttcc cagtgcggac gaagatttcg tgccggttgt
     3721 gccagactga gcgccgccag ttgaggttgc gctttttctt cttcggctaa cgcctgttgc
     3781 aaggcctgct gacggcggct ggcttcttgc tgcaattcgt ccagacgcgt taaccagttt
     3841 agtgattgtt gttcttgctg ctgcgcggta agtaactgtt tttcttcgtc agtaagtacc
     3901 tgcaaactcg ctgtcagcga ttgcacttgt tccggcgtga gcaacgcgac gccgctggcc
     3961 tgcgcttgca gcttctccag ctctgtgcgg gctgatttgt gctgctcaaa aaccatcgcc
     4021 gagatttgcc cgtagatttc agtgccagtt aactcctcga gcaattccgc gcgttctttg
     4081 ggtttggcat tcaggaaggc agcaaattgc ccctgcgaaa gcagcatcga acgggtgaag
     4141 cgcccgtaat ccagtccggt taacgtcgcc gtcagttcca gcttatcttt cactttgtcg
     4201 gcgagaattt tgccatcggc gcagcgcgcc agctctacgc gtggcacctg caaattaccg
     4261 tccggctggt tacgcgcccg attctggctc cagaacgcgc gatacgcttc acctttcact
     4321 tcaaactcca cctccgccag acattcggcg gtatcgcggg tcatgagatc attttgcgat
     4381 tgtgaaacgt tagagaggcg cggagtttcg tgatacagcg ccagacaaat ggcgtccagc
     4441 agggtggttt tccccgcacc ggttgggccg gtaatggcaa acagcccgtt gctggcgaac
     4501 ggctcgcggg tgaaatcaat cttccattcg ccttttaatg agttcaggtt tttcaggcgc
     4561 aggctgagaa ttttcatact tcgtgttctc cggcgagggt atgcaacgtc gtggtgaaaa
     4621 gatactgcag acgttgctgc tgcgattcat ccagttcttc cagtgccaga cggcgattga
     4681 acacctcttc gacgctgagt tcgctgaggg tttcacgctg ttggctggct aacacacgct
     4741 cgcgctgttc acgactccgg cgtaccagca atacttcgac aggcaatgat tcggttaatg
     4801 cctggatttt gcgctgaata tcatgcagat actcatcagt agtgatttcg atatccagcc
     4861 agacaggtgg ctcctgtgat acatcgcgcc actgttccag ctgttcggta atcgacgcca
     4921 gatcgccttt cagcaccgcc atgggttgcg ttaccggtac gttcaggttt tccacgctct
     4981 ctaatttgcc gtttgaaaat gtcaccagat ggacatattt actcttaccg cattcatcaa
     5041 aactcagtgg aatgggggaa ccgcaatagc gaacatgttc catgccgcca ataatctgtg
     5101 cgcggtgaat atgcccgagc gcgatgtagt cggctggtgg aaagttttgt gccggaaacg
     5161 cgtccagcgt gccaatataa atgtcacgca cggcgtcact tttactggca cccacggtcg
     5221 ttaaatgtcc cgtggcgatg atgggcagag gctgatcgcc gcgcagtttg caggcatcgg
     5281 catagtgttg ttggtaataa tcggtaatcg ctgccagcaa atgctgctgt ttttcaatac
     5341 cgttaagccc cgcctggctg gtaataatgt cacgcggacg taaaaacgga atggggcaca
     5401 gcactgcgcc tggcgtcccg tcgcgacgag gcaagatttg cggcgcatgt ccggcgctgg
     5461 cgaccacggt cgtattgagg aacgccatga tatcgcgcga ttcattcagc gtggcgaccg
     5521 agtcatggtt tcctgccagt accaccagat gacagccagt ttgctgtaaa ttgacaacaa
     5581 aacggttgta taacgtgcgg gcgtaactgg gcggcgagcc ggtatcgaaa acatcaccag
     5641 caacaataat cgcatccacc tgatgggttt gtgctgtctc cagcagccag tcaagaaaag
     5701 cctgatgttc agcttcgcgg cttttactgt agaagttctg gccgagatgc cagtctgagg
     5761 tgtgaaggat gcgcataacg gttccctggc gaaaaagcat gggcgcgatt atacccaaac
     5821 agatgtgcca tttgcttttt tctgcgccac ggaaatcaat aacctgaaga tatgtgcgac
     5881 gagcttttca taaatctgtc ataaatctga cgcataatga cgtcgcatta atgatcgcaa
     5941 cctatttatt acaacagggc aaatcatggc gagacgtatt ctggtcgtag aagatgaagc
     6001 accaattcgc gaaatggtct gcttcgtgct cgaacaaaat ggctttcagc cggtcgaagc
     6061 ggaagattat gacagtgctg tgaatcaact gaatgaaccc tggccggatt taattctcct
     6121 tgactggatg ttacctggcg gctccggtat ccagttcatc aaacacctca agcgcgagtc
     6181 gatgacccgg gatattccag tggtgatgtt gaccgccaga ggggaagaag aagatcgcgt
     6241 gcgcggcctt gaaaccggcg cggatgacta tatcaccaag ccgttttcgc cgaaggagct
     6301 ggtggcgcga atcaaagcgg taatgcgccg tatttcgcca atggcggtgg aagaggtgat
     6361 cgagatgcag gggctaagcc tcgacccgac atctcaccgg gtgatggcgg gcgaagagcc
     6421 actggagatg gggccgacag aatttaaact gctgcacttt tttatgacgc atcctgagcg
     6481 cgtgtacagc cgcgagcagc tgttaaacca cgtctgggga accaacgtgt atgtggaaga
     6541 tcgtacggtc gatgtccaca ttcgtcgcct gcgtaaagca ctggagcccg gtgggcatga
     6601 ccgcatggtg cagaccgtgc gcggtacagg atatcgtttt tcaacccgct tttaacgcct
     6661 tgctcatcgg acgcagagca gggcttatga tttcttaact ggagtatctt acgtgctgga
     6721 acggctgtcg tggaaaaggc tggtgctgga gctgctactt tgttgcctcc cggctttcat
     6781 cctgggtgca ttttttggtt acctgccctg gtttttgctg gcatcggtaa cgggactgct
     6841 tatctggcat ttctggaatt tattgcgcct ttcatggtgg ctgtgggtgg atcgtagtat
     6901 gaccccgcca ccggggcgtg gtagctggga accgctacta tacggcttac accagatgca
     6961 gctgcgaaat aaaaaacgcc gccgtgaact gggcaatctg attaaacgct ttcgtagcgg
     7021 cgcggagtcg ctacccgacg cagtggtgct gaccacggaa gagggcggta ttttctggtg
     7081 taacggtctg gcgcaacaga ttcttggttt gcgctggccg gaagataacg ggcagaacat
     7141 ccttaacctg ctgcgttacc cggagtttac gcaatatctg aaaacgcgtg atttttctcg
     7201 cccgctcaat ctggtgctca acaccgggcg gcatctggaa attcgcgtca tgccttatac
     7261 ccacaaacag ttgctgatgg tggcgcgtga tgtcacgcaa atgcatcaac tggaaggggc
     7321 gcggcgtaac ttttttgcca acgtgagcca tgagttacgt acgccattga ccgtgttaca
     7381 gggttacctg gagatgatgg atgagcaacc gctggaaggc gcggtacgtg aaaaagcgtt
     7441 gcacaccatg cgcgagcaga tccagcggat ggaagggctg gtgaagcaat tgctgacgct
     7501 gtcgaaaata gaagccgcac cgacgcattt gctcaatgaa aaggttgatg tgccaatgat
     7561 gctgcgcgtt gttgagcgcg aggctcagac tctgagtcag aaaaaacaga catttacctt
     7621 tgagatagat aacggcctca aggtgtctgg caacgaagat cagctacgca gtgcgatttc
     7681 gaacctggtc tataacgccg taaatcatac gccggaaggc acgcatatca ccgtacgctg
     7741 gcagcgagtg ccgcacggtg ccgaatttag cgttgaagat aacggaccgg gcattgcacc
     7801 ggagcatatt ccgcgcctga ccgagcgttt ttatcgcgtt gataaagcgc gttcccggca
     7861 aaccggcggt agcggattag ggttagcgat cgtgaaacat gcggtgaatc atcacgaaag
     7921 tcgcctgaat attgagagta cagtaggaaa aggaacacgt ttcagttttg ttatcccgga
     7981 acgtttaatt gccaaaaaca gcgattaatc cgcctttgtc atcttttatt gccataagcc
     8041 agccgatgct ggcttatttt ctttgcagtc aaaatacggg cgttagattt tacaacggtt
     8101 ggtgattttt tgttcgcatg attagccatg tctttttcac ggaaatagtg ttttatactg
     8161 gttggtgatt tcttatcgct atatacctct ggtttttaga tcccttcttg ctttaaaacg
     8221 ttataagcgt ttaaattgcg cttcaggtgc tgtcatactg actgcattca cgcggtaaat
     8281 cgaaaaacta ttcttcgccg cgcctggttg ggagtatttc ccgctaaaat tgtttaaata
     8341 taccgctgta tcatccccag ggattggcac aaaaatttaa cgttacaaca ccacatccac
     8401 aggcagtatg atttatgacc catcaattaa gatcgcgcga tatcatcgct ctgggcttta
     8461 tgacatttgc gttgttcgtc ggcgcaggta acattatttt ccctccaatg gtcggcttac
     8521 aggcaggcga acacgtctgg actgcggcat tcggcttcct cattactgcc gttggcctgc
     8581 cggtattaac ggtagtggcg ctggcaaaag ttggcggcgg tgttgacagc ctcagcacgc
     8641 caatcggtaa agtcgctggc gtgctgctgg caacggtgtg ttacctggcg gtggggccgc
     8701 ttttcgctac gccgcgtaca gctaccgttt cttttgaagt gggcattgcg ccgctgacgg
     8761 gtgattccgc actgccgctg tttatctaca gcctggttta tttcgctatc gttattctgg
     8821 tttcgctcta tccgggcaaa ctgctggata ccgtgggcaa cttccttgcg ccgctgaaaa
     8881 ttatcgcgct ggtcatcctg tctgttgccg cgattgtctg gccggcgggt tctatcagca
     8941 cggcgactga ggcttatcaa aacgctgcgt tttctaacgg cttcgtcaac ggctatctga
     9001 ccatggatac gctgggcgcg atggtgtttg gtatcgttat tgttaacgcg gcgcgttctc
     9061 gtggcgttac cgaagcgcgt ctgctgaccc gttataccgt ctgggctggc ctgatggcgg
     9121 gtgttggtct gactctgctg tacctggcgc tgttccgtct gggttcagac agcgcgtcgc
     9181 tggtcgatca gtctgcaaac ggcgctgcta ttctgcatgc ttacgttcag catacctttg
     9241 gcggcggcgg tagcttcctg ctggcggcgt taatcttcat cgcctgcctg gtcacggcgg
     9301 ttggcctgac ctgtgcttgt gcagaattct tcgcccagta cgtaccgctc tcttatcgta
     9361 cgctggtgtt tatcctcggc ggcttctcga tggtggtgtc taacctcggc ttgagtcagc
     9421 tgattcagat ctctgtaccg gtgctgaccg ccatttatcc gccgtgtatc gcactggttg
     9481 tattaagttt tacacgctca tggtggcata attcgtcccg cgtgattgct ccgccgatgt
     9541 ttatcagcct gctttttggt attctcgacg ggatcaaagc atctgcattc agcgatatct
     9601 taccgtcctg ggcgcagcgt ttaccgctgg ccgaacaagg tctggcgtgg ttaatgccaa
     9661 cagtggtgat ggtggttctg gccattatct gggatcgcgc ggcaggtcgt caggtgacct
     9721 ccagcgctca ctaaatcact gaacatttgt tttaaccacg gggctgcgat gccccgtggt
     9781 tttttattgt gttgatgggt taggaattga tggaaagtaa gaacaagcta aagcgtgggc
     9841 taagtacccg ccacatacgc tttatggcac tgggttcagc aattggcacc gggttgtttt
     9901 acggttcggc agacgccatc aaaatggccg gtccgagcgt gttgttggcc tatattatcg
     9961 gtggtgtcgc ggcgtatatc attatgcgcg cgctggggga aatgtcggta cataacccgg
    10021 ccgccagctc tttctcgcgt tatgcgcagg aaaacctcgg accgctggca ggttacatta
    10081 ccggctggac ctactgcttt gaaatcctta ttgtcgccat cgccgatgtg accgcttttg
    10141 gtatctatat gggtgtctgg ttcccgacgg tgccgcactg gatttgggta ctgagcgtgg
    10201 tgctgatcat ttgcgccgta aacctgatga gcgtgaaggt attcggtgag ctggagttct
    10261 ggttctcgtt ctttaaagtc gccaccatca tcatcatgat tgtcgccggt ttcggcatca
    10321 tcatctgggg gattggcaac ggcgggcaac cgaccggtat tcataacctg tggagcaacg
    10381 gcggcttctt cagtaacggc tggcttggga tggtgatgtc gttgcaaatg gtgatgtttg
    10441 cttacggtgg gatcgaaatt atcgggatta ccgccggtga agcgaaagat cctgagaaat
    10501 cgataccgcg tgcgattaac tccgtgccga tgcgtattct ggtgttctac gtcggtacgc
    10561 tgttcgtcat tatgtctatc tacccgtgga atcaggttgg cactgccggt agcccgttcg
    10621 tgctgacgtt ccagcatatg ggcattacct ttgccgccag cattcttaac tttgttgtgc
    10681 tgactgcttc gctgtcggca attaacagtg acgtatttgg cgtaggccgt atgctccacg
    10741 gtatggcaga gcagggcagc gcgccgaaaa ttttcagcaa aacgtcgcgt cgcggtattc
    10801 cgtgggttac ggtgctggtg atgactaccg cgctgctgtt tgcggtgtat ctgaactaca
    10861 tcatgccgga aaacgtcttc ctggtgatcg cttcgctggc aaccttcgcc acggtgtggg
    10921 tgtggattat gatcctgctg tcgcaaattg ccttccgtcg ccgtttgccg ccagaagaag
    10981 ttaaggcgct gaaatttaaa gtgccgggtg gggtagcaac gaccatcggc gggctgattt
    11041 tcctgctctt tattatcggg ttgattggtt atcacccgga tacgcgtatc tcgctgtatg
    11101 tcggtttcgc gtggattgtt gtgctgttga ttggctggat gtttaaacgc cgccacgatc
    11161 gtcagctggc tgaaaaccag taatacttgc gttctgtaag ccggttcagg aggaaatcct
    11221 gatccggcta ttccgaaagt tatccgcccc cgtcctcctc ccccaaatat ccttcagatg
    11281 atgagtgatc ctgcattagg ctatggcaag gtgatcagat tttcatcaca ggggaattat
    11341 gatgttaaat gcatggcacc tgccggtgcc cccatttgtt aaacaaagca aagatcaact
    11401 gctcattaca ctgtggctga cgggcgaaga cccaccgcag cgcattatgc tgcgtacaga
    11461 acacgacaac gaagaaacgt cagtaccgat gcataagcag cgcagtcagc cgcagcctgg
    11521 cgtcaccgca tggcgggcgg cgatcgatct ctccagcggg caaccccggc ggcgttatag
    11581 tttcaaattg ctgtggcacg atcgccagcg ttggtttaca ccgcagggct tcagccgaat
    11641 gccgccagca cgactggagc agtttgccgt cgatgtcccg gatatcggcc cacaatgggc
    11701 tgcggatcag attttttatc agatcttccc tgatcgtttc gcccgtagtc ttcctcgtga
    11761 agctgaacag gatcatgttt attaccatca cgcagccgga caagagatca tattgcgtga
    11821 ctgggatgaa ccggtcacgg cgcaggcggg cggatcaacg ttctatggcg gcgatctgga
    11881 cggtatcagc gaaaaactgc cgtatctgaa aaaacttggc gtgacggcgc tgtatcttaa
    11941 tccggtgttt aaagctccca gcgtacataa atacgatacc gaggattatc gccatgtcga
    12001 tccgcagttt ggcggtgatg gggcattgct gcgtttgcga cacaatacgc agcagctggg
    12061 aatgcggctg gtgctggatg gcgtgtttaa ccacagtggc gattcccatg cctggtttga
    12121 caggcacaat cgtggcacgg ggggagcttg tcacaacccc gaatcgccct ggcgcgactg
    12181 gtactcgttt agtgatgatg gcacggcgct cgactggctg ggctatgcca gcttgccgaa
    12241 actggattat cagtcggaaa gtctggtgaa tgaaatttat cgcggggaag acagtattgt
    12301 ccgccactgg ctgaaagcgc cgtggaatat ggacggctgg cgactggatg tggtgcatat
    12361 gctgggggaa gccggtgggg cgcgcaataa tttgcagcac gttgccggga tcaccgaagc
    12421 ggcgaaagaa acccagccgg aagcgtatat agttggagag cattttggcg atgcacggca
    12481 atggttacag gccgatgtgg aagatgccgc catgaactat cgcggcttca cattcccgtt
    12541 gtggggattt ctcgccaata ccgatatctc ttatgatccg cagcaaattg acgcccaaac
    12601 ctgtatggcc tggatggata attaccgcgc aggactttct catcaacagc aattacgtat
    12661 gtttaatcag ctcgatagcc acgatactgc gcgatttaaa acgctgctcg gtcgggatat
    12721 tgcgcgcctg ccgctggcgg tggtctggct gttcacctgg cctggtgtac cgtgcattta
    12781 ttacggtgat gaagtaggac tggatggcaa aaacgatccg ttttgccgta aaccgttccc
    12841 ctggcaggtg gaaaagcagg atacggcgtt attcgcgttg taccagcgaa tgattgcgct
    12901 gcgtaagaaa agtcaggcgc tgcgtcgtgg cggctgtcag gtgctgtatg cggaagataa
    12961 cgtggtggta tttgtccgcg tgctgaatca gcagcgtgta ctggtggcaa tcaaccgtgg
    13021 cgaggcctgt gaagtggtgc tacccgcgtc accgtttctt aatgtcgtgc aatggcaacg
    13081 caaagaaggc catgggcaac tgacagacgg gattctggct ttgcctgcca tttcggctac
    13141 ggtgtggatg aactaaagca tctgttttat ttgtaacgtc gttagtcacc agccctgaca
    13201 ctacgcagcg aaaaagctgc tcaatgtcag ggctgaattt gttaaatatt tgtcctgatc
    13261 gatatgagcg atttttggta ttctttgccc atgatgttat gtacaacatg ctccaggata
    13321 accctgtagt ggagcaaaga tatgacaatt tcaaattcga atttattgaa accgtttatt
    13381 actggtagtg agttgccacc ttttaaagag gatgtctttt ctaaaacaga agaacaattt
    13441 aaaaaaagcc cccatggcaa attgcttttc atttccggaa atgaaaactt agtaaacatt
    13501 tcagcggaaa aaatcgatga aataaaaaat agcaaagatt tcttttcaaa cattgtaaat
    13561 ataataattt tactcattaa gcatcctgat aaaattctgc ccattcttca tggttgtcct
    13621 gcagagtcca tcgaactcat taagaatgcg aaacgaacgg ggcaatggtc atatgagatg
    13681 actgataaga acgggcagag tattgaagtg tcctatattt tcactgatac catagataat
    13741 aattatgatg gaaaattatg cttaaaaatc tgtaacccct gcaaccaaaa tgattctagt
    13801 acacatgaat ataaaataga tcatcaacag ttgaaaaatc acataaggca acttgaggag
    13861 tgggcccttc ttgaaaagtt ttatgataac aacgcttatt attctaaacg tacaaaacaa
    13921 ttagagaaga aaattgccga tgcggggttt ataataggga aggttaatgc cgataacttt
    13981 aatgtaaaag agtttaaaga cgatatagcc tcgatgcttt gtgaacagcc ccccatgcgt
    14041 acacaccaga tttcttcatc cggatactta tctgccgata aagtgcccga tctctataag
    14101 acaaatttga aagacgcgca gagagatctg aaaacaacac cggttgacat cgatggtgtt
    14161 agtctaaaac caaaaactcg ggaaaaaatt gcggaaaaaa ttgcgaaaaa aactgaggaa
    14221 gaaaatgcga aaaaaattgc gaaacaaaat gcgaagacca ttgaggagaa gaatagtgaa
    14281 aaaaacaaga aagaaacaaa tcctgaggat aattttgtct taaaattctc accggaagaa
    14341 atcaatgaga tgctcctgga ttacagcgat gaagaacaaa aaaaattaag accttttttc
    14401 actagtgatc atatctattg tttgtttgat gcttttagtc agggtttgta taatgttgct
    14461 cgtaatggca gtccacggta tgcaggttta ctacgtcaaa tagcatccaa gtgtgcgata
    14521 ttacaagatt ttgatcaaca attgttctgg cgcacgtttt caggaggagt cttccatcga
    14581 cgtatctctg tgaacagcga gccagaaaag acagatgtat ggcgattcag tacacaattt
    14641 gattttccat atataaatga aaacggagag ttgtccagaa cggtactcat gttcgagggg
    14701 gttgctgaaa gcaactttaa agatatcgcc aatcattcca gcgaatgttt ttcttacagc
    14761 tgttatgccg ccccgctgga tgcgaatgct aaaaaatagt gacgccggag cttgaagttt
    14821 cagagcgcct tgtgtgacgc ctgtgccatc atccgcggat aaaactgcca gaagcgggtt
    14881 tccagggcat cgtaatgggc gtctaaatcg taccaggagt cacgcagggc atccagacgt
    14941 gggcgacggc ttgccatgcc gtttaacacg ttctggatga aatccatatc tcgatagcgc
    15001 accagccact gctctgacca caagtaattg ttcagattga taaaacgtgg cggtgagtcc
    15061 ggcaaaatcg tcatcacttg ctcgcgggca taacagacaa attcctgtag cggaaagtcc
    15121 ggcgacagct gcgaccagtg gcgggaaaga aagtgatccc acatgacatc cagggtaata
    15181 ggcgcaacgc ggcgcgtttc actacgaaac cattcccgtg cttcgcggac ttccggcaga
    15241 ttgtcggtta gtacatcaat gcgccgatgc atatgaatgc cagccacgac gtcgggcgga
    15301 aaactttctt caggatttcc gcgtacgaaa tcagccagta aattgccaga aagcgagctt
    15361 tccgccaggt gagctagatg caggtgagct aaaaaattca tatacacttc atccttcaaa
    15421 ctgcctcttc gttggctgcc ttcactcacc ccagtcactt acttaatgta agctcctggg
    15481 gactcattca gttgccatct cgatgcatct tgaatgattt tgtgtataga ttatgtcatc
    15541 catcaaggag ggtaaaggtt gcagggagag caccccggca ctagactacc cgcctcttat
    15601 tttagtctga gtcagtgtca tgcgcgttac cgatttctcc tttgaattgc ccgaatccct
    15661 gattgcccac tatcccatgc ctgaacgcag tagctgtcgt ttactgtcgc tggacgggcc
    15721 gacgggcgcg ctgacgcacg gtactttcac cgatttactc gataagctca accccggcga
    15781 tcttctggtt tttaataata cccgcgtgat cccggcgcgc ctgtttgggc gtaaagccag
    15841 cggcggcaag attgaagtgc tggttgagcg gatgctcgac gacaaacgca ttcttgcgca
    15901 tattcgcgcc tcgaaagcgc caaaacctgg cgcagaactg ttactgggcg atgacgaaag
    15961 tatcaacgca acaatgaccg cgcgccacgg cgcactgttt gaagttgaat ttaatgatga
    16021 tcgttcggta ctggatatcc tcaacagcat cggccatatg ccgctgccgc cgtatatcga
    16081 ccgtccggac gaagacgctg accgcgaact ttatcaaacc gtttatagcg aaaaaccggg
    16141 cgcggttgca gccccgaccg ctggtctgca ttttgacgaa cctttgctgg aaaaattacg
    16201 cgccaaaggc gtggagatgg cgttcgtcac cctgcacgtt ggtgcgggca ccttccagcc
    16261 ggtgcgcgtc gacaccattg aagatcacat catgcactcg gaatacgctg aagtaccgca
    16321 ggatgtggta gacgcggtac tggcggcgaa agcgcgcggt aaccgggtga ttgcggttgg
    16381 caccacctca gtacgttcac tggaaagcgc ggctcaggcg gcgaaaaacg atctcatcga
    16441 accgttcttt gacgacaccc aaatctttat ctatccgggc tttcagtaca aagtggtcga
    16501 tgcgctggtg acgaacttcc acttgccaga gtcgacgctg attatgctgg tttcggcctt
    16561 tgccggttat caacacacca tgaacgccta taaatcagcg gtagaagaga aatatcgctt
    16621 ttttagttac ggtgatgcga tgtttatcac gtacaatccg caggcaatta atgagcgcgt
    16681 cggggagtaa ttccgcggcg ctggtttaaa acgttggact gtttttctga cgtagtggag
    16741 aaaaaatgaa atttgaactg gacaccaccg acggtcgcgc acgccgtggc cgcctggtct
    16801 ttgatcgtgg cgtagtggaa acgccttgtt ttatgcctgt tggcacctac ggcaccgtaa
    16861 aagggatgac gccggaagaa gttgaagcca ctggcgcgca aattatcctc ggcaacacct
    16921 tccacctgtg gctgcgcccg ggccaggaaa tcatgaaact gcacggcgat ctgcacgatt
    16981 ttatgcagtg gaaaggccca atccttaccg actccggcgg cttccaggtc ttcagccttg
    17041 gtgatattcg taaaatcacc gaacagggcg ttcacttccg taacccgatc aacggcgacc
    17101 cgattttcct cgacccggaa aagtcgatgg agattcagta cgatcttggt tcggatatcg
    17161 tcatgatctt tgatgagtgt acgccgtatc ctgctgactg ggattacgca aaacgctcca
    17221 tggagatgtc tctgcgttgg gcgaagcgta gccgtgagcg ttttgatagt ctcggaaaca
    17281 aaaatgcgct gtttggtatc attcagggca gcgtttacga agatttacgt gatatttctg
    17341 ttaaaggtct ggtagatatc ggttttgatg gctacgctgt cggcggtctg gctgtgggtg
    17401 agccgaaagc agatatgcac cgcattctgg agcatgtatg cccgcaaatt ccggcagaca
    17461 aaccgcgtta cctgatgggc gttggtaaac cagaagacct ggttgaaggc gtgcgtcgcg
    17521 gtatcgatat gtttgactgc gtaatgccaa cccgcaacgc ccgaaatggt catttgttcg
    17581 tgaccgatgg cgtggtgaaa atccgcaatg cgaagtataa gagcgatact ggcccactcg
    17641 atcctgagtg tgattgctac acctgtcgca attattcacg cgcttacttg catcatctcg
    17701 accgttgcaa cgaaatatta ggcgcgcgac tcaataccat tcataacctt cgttactacc
    17761 agcgtttgat ggcgggttta cgcaaggcta ttgaagaggg taaattagag agcttcgtaa
    17821 ctgattttta ccagcgtcag gggcgagaag taccaccttt gaacgttgat taatattaat
    17881 aatgagggaa atttaatgag cttttttatt tctgatgcgg tagcggcaac gggtgcaccg
    17941 gcgcaaggta gcccgatgtc tttgattttg atgctggtgg tattcggtct gattttctat
    18001 ttcatgatcc tgcgtccaca gcagaagcgc accaaagaac acaaaaagct gatggactcc
    18061 attgccaaag gtgatgaagt tctgacgaac ggtggcctgg ttggtcgcgt aaccaaagta
    18121 gcggaaaacg gctacattgc tatcgcgctg aatgacacca ctgaagtagt tattaaacgt
    18181 gacttcgtag ctgccgtcct gccgaaaggc accatgaagg cgctgtaatt aaaatttttc
    18241 cctaagggaa ttgccgtgtt aaaccgttat cctttgtgga agtacgtcat gctgatcgtg
    18301 gtgattgtca tcggtctgct gtatgcgctt cccaacctgt ttggtgagga tccggctgtt
    18361 cagatcactg gtgcgcgcgg agtcgccgcc agtgagcaaa cgctgatcca ggtccagaaa
    18421 acgttacaag aagaaaaaat aactgctaag tctgtggcac tggaagaggg cgctattctt
    18481 gcgcgcttcg actccactga cacccagctg cgcgctcgtg aagcattaat gggcgttatg
    18541 ggtgacaaat acgtcgtggc gcttaacctt gccccggcaa cgccgcgctg gctggcagct
    18601 attcacgctg agccgatgaa gctcggcctt gacctgcgtg gcggcgttca cttcctgatg
    18661 gaagtggata tggacaccgc gcttggcaaa ctccaggaac aaaatatcga tagcctgcgc
    18721 agcgacctgc gcgaaaaggg catcccgtat accaccgttc gtaaagaaaa caactacggc
    18781 ctgagcatca ctttccgcga tgctaaagct cgtgatgaag ccattgcgta tttgagcaag
    18841 cgtcatccgg acctggtgat tagtagccag ggcagcaacc agctgcgtgc ggtaatgagc
    18901 gatgctcgcc ttagtgaagc gcgtgaatat gcggtgcagc agaacattaa tatcctgcgt
    18961 aaccgtgtaa accaacttgg tgtggcggag ccggtggttc agcgtcaggg tgctgaccgt
    19021 atcgttgttg aactgccagg tattcaggac accgcgcgtg cgaaagagat tctgggtgcg
    19081 acggcaacgc tggaattccg tctggtaaac actaacgttg atcaggccgc agcggcatcc
    19141 ggtcgcgtac ctggcgactc tgaagtgaaa cagacccgcg aaggtcagcc agttgtgctg
    19201 tacaaacgcg taattctgac cggtgaccat atcaccgact ccacttccag ccaggacgaa
    19261 tacaaccagc cgcaggttaa catctcgctc gacagcgcag gtggcaacat tatgtctaac
    19321 ttcaccaagg acaacatcgg caaaccgatg gcgaccctgt ttgtggagta caaagacagc
    19381 ggcaagaaag atgctaacgg tcgtgcggtt ctggtgaaac aggaagaggt gattaacatc
    19441 gctaacatcc agtctcgtct gggtaacagc ttccgtatca ccggtattaa caacccgaac
    19501 gaagcccgtc agctttcact tttgctgcgt gccggtgcgt tgatcgcgcc gattcagatt
    19561 gttgaagaac gtaccattgg cccgaccctg ggtatgcaga acattgagca ggggctggag
    19621 gcttgccttg ccggtctgct ggtttctatt ctgttcatga tcatcttcta taagaagttt
    19681 ggtctgattg cgaccagtgc tctgattgcc aacttgatct tgattgtcgg cattatgtcg
    19741 ctgttgccag gcgcaacgct gagtatgcca ggtatcgcgg gtatcgtctt aacccttgcg
    19801 gtagcggtcg atgcgaacgt acttatcaac gaacgtatta aagaagagtt gagcaacgga
    19861 cgtactgttc aacaggcaat tgatgaaggt tatcgtggcg cattcagttc catcttcgat
    19921 gcgaacatca ccacgctgat taaagtcatc atcctgtacg cagtgggtac cggggcaatt
    19981 aaagggttcg cgattactac cggtatcggt gtggcgacgt cgatgtttac cgcgattgtc
    20041 ggtacgcgtg ccatcgtaaa cctgctatat ggcggcaagc gcgtcaagaa gctgtcaatc
    20101 tgaggagtgc gatgtggcac aggaatatac tgttgaacaa ctaaaccacg gccgtaaagt
    20161 ctatgacttt atgcgctggg actactgggc ttttggcatc tctggtctgc tgttaatcgc
    20221 cgctatcgtt attatgggcg tgcgcggctt taactggggg ctggatttca ccggtggtac
    20281 ggttatcgaa attacgctcg aaaaaccggc tgaaattgac gtaatgcgtg atgcattgca
    20341 aaaagccggt tttgaagagc cgatgctgca aaactttggt agcagccacg acatcatggt
    20401 ccgtatgccg cctgctgaag gcgaaaccgg cggtcaggtg ctgggcagcc aggttctgaa
    20461 ggtgattaac gaatccacca atcagaatgc ggcagtgaag cgtattgagt tcgtcggtcc
    20521 gagcgtgggg gcagaccttg cgcaaaccgg tgcgatggcg ttgatggcgg cgctgctatc
    20581 tatcctcgtg tacgtaggtt tccgctttga gtggcgactg gcggcagggg ttgttattgc
    20641 gctggcgcac gacgttatca ttacgctggg tattttgtcg ttattccata tcgagattga
    20701 cctgaccatt gtggcatcgt tgatgtcggt tatcggttac tcgcttaacg acagtatcgt
    20761 ggtatcagac cgtattcgcg aaaacttccg caagatccgt cgcggtacgc cttacgaaat
    20821 ctttaacgtg tccttgaccc agacgctgca ccgtaccttg atcacatccg gtactacctt
    20881 gatggttatc ctgatgctgt acctcttcgg tggtccggta ctggaaggct tctcgctgac
    20941 catgcttatc ggtgtttcca tcggtactgc atcttccatc tatgtggcat cggcgctggc
    21001 tctgaaactg ggtatgaagc gcgaacacat gctgcagcag aaagtggaaa aagaaggggc
    21061 ggatcagccg tcaattctgc cgtaatcaag ttcccgttga tgttgaaaat cccggtcaga
    21121 agatcgggat ttttttgtgc acagaaaacc cccagctagg c
//
LOCUS       DAPOZO010000086        20186 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPOZO010000086 DAPOZO010000000
VERSION     DAPOZO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.086"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            8..889
                     /locus_tag="ROI21_003479"
     CDS             8..889
                     /locus_tag="ROI21_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5112361.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAIEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1094..2563
                     /gene="lysP"
                     /locus_tag="ROI21_003480"
     CDS             1094..2563
                     /gene="lysP"
                     /locus_tag="ROI21_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDX5112362.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2855..4834
                     /gene="cirA"
                     /locus_tag="ROI21_003481"
     CDS             2855..4834
                     /gene="cirA"
                     /locus_tag="ROI21_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDX5112363.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEDGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4865..5701)
                     /gene="yeiG"
                     /locus_tag="ROI21_003482"
     CDS             complement(4865..5701)
                     /gene="yeiG"
                     /locus_tag="ROI21_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDX5112364.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDDNFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALIQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPRYDHSYYFIASFIEDHLRFHAQHL
                     LK"
     gene            5959..6627
                     /gene="folE"
                     /locus_tag="ROI21_003483"
     CDS             5959..6627
                     /gene="folE"
                     /locus_tag="ROI21_003483"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDX5112365.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6644..7801
                     /locus_tag="ROI21_003484"
     CDS             6644..7801
                     /locus_tag="ROI21_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDX5112366.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLMLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMLFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPMEWLWRQLTLRAAGPAISKTSR"
     gene            7943..8983
                     /gene="galS"
                     /locus_tag="ROI21_003485"
     CDS             7943..8983
                     /gene="galS"
                     /locus_tag="ROI21_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDX5112367.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVGLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMGNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNLAM"
     gene            9263..10261
                     /gene="mglB"
                     /locus_tag="ROI21_003486"
     CDS             9263..10261
                     /gene="mglB"
                     /locus_tag="ROI21_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDX5112368.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10322..11842
                     /gene="mglA"
                     /locus_tag="ROI21_003487"
     CDS             10322..11842
                     /gene="mglA"
                     /locus_tag="ROI21_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDX5112369.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11858..12868
                     /gene="mglC"
                     /locus_tag="ROI21_003488"
     CDS             11858..12868
                     /gene="mglC"
                     /locus_tag="ROI21_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDX5112370.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12939..14174)
                     /gene="preA"
                     /locus_tag="ROI21_003489"
     CDS             complement(12939..14174)
                     /gene="preA"
                     /locus_tag="ROI21_003489"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDX5112371.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQDMVGLANNNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(14168..15406)
                     /gene="preT"
                     /locus_tag="ROI21_003490"
     CDS             complement(14168..15406)
                     /gene="preT"
                     /locus_tag="ROI21_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDX5112372.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            complement(15725..15964)
                     /locus_tag="ROI21_003491"
     CDS             complement(15725..15964)
                     /locus_tag="ROI21_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDX5112373.1"
                     /translation="MDVQHFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(15967..16686)
                     /gene="sanA"
                     /locus_tag="ROI21_003492"
     CDS             complement(15967..16686)
                     /gene="sanA"
                     /locus_tag="ROI21_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDX5112374.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16836..17720)
                     /gene="cdd"
                     /locus_tag="ROI21_003493"
     CDS             complement(16836..17720)
                     /gene="cdd"
                     /locus_tag="ROI21_003493"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDX5112375.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(17850..18545)
                     /locus_tag="ROI21_003494"
     CDS             complement(17850..18545)
                     /locus_tag="ROI21_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDX5112376.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(18542..18940)
                     /locus_tag="ROI21_003495"
     CDS             complement(18542..18940)
                     /locus_tag="ROI21_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDX5112377.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19179..20126
                     /gene="dusC"
                     /locus_tag="ROI21_003496"
     CDS             19179..20126
                     /gene="dusC"
                     /locus_tag="ROI21_003496"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDX5112378.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPIK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSHDIARAIQAIDIEKL"
BASE COUNT         4966 a         4986 c         5252 g         4982 t
ORIGIN      
        1 agcgactatg cacatcaccc tccggcagtt ggaagttttt gcagaagtat tgaaaagtgg
       61 atcaaccacc caggcgtcgg tgatgctggc gttgtcgcaa tcagcagtga gcgcagcctt
      121 gaccgacctg gaagggcagc ttggcgtgca actgtttgat cgcgtgggga aaagactggt
      181 tgttaatgaa cacgggcggc tgctctatcc gcgcgcgttg gcactgcttg aacaggcgat
      241 cgaaatcgaa caactgtttc gcgaagacaa cggtgcgatt cgtatctatg ccagtagtac
      301 catcggtaac tacattctgc ctgcagttat cgcccgttat cgccatgatt atccgcagtt
      361 gccgattgaa cttagcgttg ggaacagcca ggacgtgatg caagcggtgc tggatttccg
      421 cgttgatatt ggctttattg aaggaccgtg ccacagcact gaaatcatct cagaaccgtg
      481 gctggaagac gagctggtgg ttttcgccgc gccgacttcg ccattggctc gtggacctgt
      541 caccttagaa cagctggccg ctgcgccgtg gatcctgcgt gaacgcggct ccggcacgcg
      601 ggagattgtc gattatctgt tgctgtcaca tttaccgaag tttgagatgg cgatggaatt
      661 aggtaactcc gaggcaatca aacatgcggt gcgtcatggg ttgggaatta gttgcctgtc
      721 gcgacgggtg attgaagatc aattgcaggc aggcacatta agtgaagttg cggtccctct
      781 gccgcgcctg atgcgtacgt tgtggcgtat acatcatcgg caaaaacacc tttccaacgc
      841 gctacggcgc tttctggact attgcgatcc cgcgaatgta ccgcgttaag ttgctgtaca
      901 agaacatgct ggtgctgtgt cgatttcgtg acgcagcgcc ctcagcatgc attcgccaga
      961 aaagagattg gctgctttac ttataatccc tgggcgatca tgaaggtgtc ttataaccgt
     1021 gtatttctgc cggaaggatt gccaatcgtc tgctacaatc gcgcctcatt tttaagatgg
     1081 atagcatttt tgtatggttt ccgaaactaa aaccacagaa gcgccgggct tacgccgtga
     1141 attaaaggcg cgtcacctga cgatgattgc cattggcggt tccatcggta caggtctttt
     1201 tgttgcctct ggcgcaacga tttctcaggc aggtccgggc ggggcattgc tctcgtatat
     1261 gttgattggc ctgatggttt acttcctgat gaccagtctc ggtgaactgg ctgcatatat
     1321 gccggtttcc ggttcgtttg ccacttatgg tcagaactat gttgaagaag gctttggctt
     1381 cgcgctgggc tggaactact ggtacaactg ggcggtgact atcgccgttg acctggttgc
     1441 agctcagctg gtcatgagct ggtggttccc ggatacaccg ggctggatct ggagtgcgct
     1501 gttcctcggc gttatcttcc tgctgaacta catctcagtt cgcggctttg gtgaagcgga
     1561 atactggttc tcactgatca aagtcacgac agttattgtc tttatcatcg ttggcgtgct
     1621 gatgattatc ggtatcttca aaggcgcgca gcctgcgggc tggagcaact ggacaatcgg
     1681 cgaagcgccg tttgctggtg gttttgcggc gatgatcggc gtagctatga ttgtcggctt
     1741 ctctttccag ggaaccgagc tgatcggtat tgctgcaggc gagtccgaag atccggcgaa
     1801 aaacattcca cgcgcggtac gtcaggtgtt ctggcgaatc ctgttgttct atgtgttcgc
     1861 gatcctgatt atcagcctga ttattccgta caccgatccg agcctgctgc gtaacgatgt
     1921 taaagacatc agcgtcagtc cgttcaccct ggtgttccag cacgcgggtc tgctctctgc
     1981 ggcggcggtg atgaacgcag ttattctgac ggcggtgctg tcagcgggta actccggtat
     2041 gtatgcgtct actcgtatgc tgtacaccct ggcgtgtgac ggtaaagcgc cgcgcatttt
     2101 cgctaaactg tcgcgtggtg gcgtgccgcg taatgcgctg tatgcgacga cggtgattgc
     2161 cggtctgtgc ttcctgacct ccatgtttgg caaccagacg gtatacctgt ggctgctgaa
     2221 cacctccggg atgaccggtt ttatcgcctg gctggggatt gccattagcc actatcgctt
     2281 ccgtcgcggt tacgtattgc agggacacga cattaacgat ctgccgtacc gttcaggttt
     2341 cttcccattg gggccgatct tcgcattcat tctgtgtctg attatcactt tgggccagaa
     2401 ctacgaagcg ttccttaaag ataccattga ctggggcggc gtagcggcaa cgtatattgg
     2461 tatcccgctg ttcctgatta tttggttcgg ctacaagctg attaaaggaa ctcacttcgt
     2521 acgctacagt gaaatgaagt tcccgcagaa cgataagaaa taagtttcct cccttccttg
     2581 ctaagccctc tcaaccgaga gggctttttc aattccattt ccctgacaaa tcatgcggat
     2641 ataaaattta acatttggat tgataattgt tatcgtttgc attatcgtta cgccgcaatc
     2701 aaaaaaggct gacaaatcag aggctgttcc ggctttctgg gatgatcacc tgcatacaaa
     2761 taagtccacc gcgatgctgc cgtacgcaag gggacgtaaa gaagatgtga gcgataccca
     2821 ttttattttc gtagttacct catggagata tggaatgttt aggttgaacc ctttcgtacg
     2881 ggtcgggctg tgtttgtccg ctatttcttg tgcatggcct gtgttagcgg tcgatgatga
     2941 tggcgaaacg atggttgtca ctgcatcttc cgttgagcaa aaccttaaag atgcacccgc
     3001 cagtatcagc gtcattaccc aggaagacct gcagcgaaaa ccggtacaga atctgaagga
     3061 tgtcctcaaa gaagtgcctg gcgtacaact gacgaacgaa ggggataacc gtaagggcgt
     3121 tagtattcgt ggtcttgaca gcagctacac cctgattctc gtcgacggta aacgcgttaa
     3181 ctcccgcaat gccgtcttcc gccacaatga tttcgatctg aactggatcc cggtcgattc
     3241 catcgaacgt attgaagtgg tccgtggccc gatgtcgtcg ctgtacggtt ccgatgcgct
     3301 cggcggtgta gtgaatatca tcaccaaaaa aatcggtcag aaatggtcgg gtaccgttac
     3361 cgtcgatacc accattcagg aacatcgcga tcgcggtgat acctataacg gtcagttctt
     3421 taccagcgga ccattaattg acggtgtgct gggaatgaaa gcttacggca gcctggcaaa
     3481 acgtgaaaag gatgacccgc aaaactcaac gaccaccgat accggagaaa cgccgcgtat
     3541 tgaaggattc tccagccgcg acggcaatgt cgaatttgcc tggacaccga atcaaaatca
     3601 cgattttact gccggatacg gtttcgaccg tcaggatcgt gattccgact cgctggacaa
     3661 aaaccgcctg gaacgccaga actactccgt cagccataat gggcgttggg attacggcac
     3721 cagcgaactg aaatactacg gtgagaaagt cgagaacaaa aaccctggca acagcagccc
     3781 gataacttcc gaaagcaata cggtcgacgg caaatacacg ttgccgctga cggcgattaa
     3841 tcagtttctc acggttggcg gtgaatggcg tcacgacaaa cttagcgatg cggtgaacct
     3901 gactggggga accagctcca aaacgtctgc cagccagtac gcgctgtttg tggaagatga
     3961 atggcggatc ttcgagccgc tggcgctgac gaccggcgta cgtatggacg atcatgaaac
     4021 ctacggtgaa cactggagtc cgcgtgccta cctggtttat aacgccaccg acaccgtaac
     4081 ggtaaaaggg ggctgggcga cggcatttaa agcaccttct ctgttgcaac ttagccctga
     4141 ctggacgagc aattcttgcc gtggcgcatg taagattgtg ggtagcccgg atctgaaacc
     4201 agaaaccagc gaaagttggg aactggggct ttactacatg ggcgaagacg gctggctgga
     4261 aggggtggag tccagcgtta ccgttttccg taacgatgtg aaagatcgta tcagcatcag
     4321 ccgtacgtct gacgtcaacg ctgcaccggg ctaccaaaac tttgttggtt ttacagcgaa
     4381 cggtgtgccg gtattcagct actacaacgt taacaaagca cgtattcagg gcgtggaaac
     4441 cgagctgaaa attccgttca acgatgaatg gaaactgtca ctcaactaca cctacaacga
     4501 tggtcgtgat gtcagcaacg gcgaaaataa acctttatcc gatctgccgt tccataccgc
     4561 taacggtacg ctggactgga aaccgctggc gctggaagac tggtcattct atgtttctgg
     4621 tcactatacc gggcagaaac gcgccgacag cgcgacggct aaaacaccgg gcggttatac
     4681 catctggaat accggtgcgg cctggcaggt gactaaagac gtcaaactgc gcgcaggcgt
     4741 gctgaacctt ggcgacaagg atctcagtcg tgacgattac agctataacg aagacggacg
     4801 tcgttacttt atggcagtgg attatcgctt ctgatgagaa gatgcccggc gaaccgggca
     4861 gactttactt cagtaaatgt tgcgcatgga agcgcaaatg atcctctata aaagaggcga
     4921 tgaagtagta actgtgatca tatctcggct gaatacgcag cgtcatcggc cacgctttct
     4981 ggcgcgcggc ttctgccagc actgcgggtt gcagctggtc ggcaagaaac tgatcattat
     5041 caccctgatc gataagcgtc gggatcgcat cctgcgcgtt actggcatac atcagcgcac
     5101 aactgtccca ttccagccat gcatttttgt cctcacctaa ataggtgcta aacgctttaa
     5161 tgccccaggg tacgctgcat ggattcacaa tcggcgcaaa ggcggaaacg ctggtgtatt
     5221 tgcccgggtt tttcagcgcc ataatcaatg caccgtgacc acccattgaa tgaccgctaa
     5281 tagcgcagcg gtcgctgaca ttaaattgcg actgaatcag cgccggtaat tcatcgcgca
     5341 gataatcata catccggtaa tgcgtcgccc aaggcggttg cgtggcgtta agataaaacc
     5401 ccgcgccctg gcctaaatcg tagccatcgt cgttggcaac ctgttcgccg cgcgggctgg
     5461 tgtctggcat taccagtaca atccccagtt ctgccgccac gcgctgggca cccgccttgg
     5521 tggtgaagtt atcgtcattg caggttaatc cggaaagcca gtacagcact ggtggcgggg
     5581 tgtgatcacg aggtggtggg agaaagatac tgaacgtcat cgggcagttt aaggtactgg
     5641 agtcgtgtcg ccagcgttgc tgccagcctt caaaacagcg gtgctcttcg agcatttcca
     5701 tgcgtggctc cttgttgtgt tgtttgcaat ttcttcatca taccgtttat tcaatgtgct
     5761 gtgagtaact ttcacttccg tatttgcata acgatgtttt aacatctgct gatgaaaggc
     5821 agcggcaatt acaataatta tcgctgtgaa tactggatta tgtgcgccgc ctcacgcaca
     5881 ataatcaggc tgtaaatcag cttaataact ttgcccccac gcagggcgga ggcgtcacac
     5941 ctgcaggaga aatcataaat gccatcactc agtaaagaag cggccctggt tcatgaagcg
     6001 ttagttgcgc gaggactgga aacaccgctg cgcccgcccg tgcatgaaat ggataacgaa
     6061 acgcgcaaaa gccttattgc tggtcatatg accgaaatca tgcagctgct gaatctcgac
     6121 ctggctgatg acagtttgat ggaaacgccg catcgcatcg ctaaaatgta tgtcgatgaa
     6181 attttctccg gtctggatta cgccaatttc ccgaaaatca ccctcattga aaacaaaatg
     6241 aaggtcgatg aaatggtcac cgtgcgcgat atcaccctga ccagcacctg tgaacaccat
     6301 tttgttacca tagatggcaa agcgacggtg gcttatatcc cgaaagattc ggtgatcggt
     6361 ctgtcaaaaa ttaaccgcat cgtgcagttc tttgcccagc gtccgcaggt gcaggaacgt
     6421 ctgacccagc aaattctcat tgcgctacaa acgctgctgg gcaccaataa cgtggctgtc
     6481 tctatcgacg cggtgcatta ctgcgtgaaa gcgcgtggca tccgcgacgc aaccagtgcc
     6541 acaacaacga cctctcttgg tggattgttc aaatccagtc agaatacgcg ccacgaattt
     6601 ctgcgcgctg tgcgtcatca caactgatta aaaggcagga accatggagc gcaacgtcac
     6661 gctcgatttt gttcgcggcg tcgccattct ggggatcctg ctattaaaca tcagcgcctt
     6721 tgggctacca aaggcggctt atctcaatcc cgcctggtac ggcgctatta cgccgcagga
     6781 tgcatggacc tgggcatttc tcgatctcat cggccaggtg aaattcctca cgctttttgc
     6841 gctgctgttt ggtgctggcc tgcaaatgtt gctgccccgt ggcagacgct ggatccagtc
     6901 gcggttaacg ctgttagtct tgctgggctt tattcacggt ttattgttct gggacggcga
     6961 tattctgctg gcttacgggc tggtgggctt aatctgctgg cggctggtgc gcgatgcgcc
     7021 atcagtaaaa agcctgttta atacaggcgt catgctttat ctggtggggc ttggcgtttt
     7081 gatgttattg gggctgattt ccgacagcca gaccagccgc gcctggacgc cggatgcatc
     7141 ggctattttg tatgaaaaat actggaagct tcacggcggc gttgaagcga tcagtaatcg
     7201 tgccgacggt gttggcaaca gtttactggc actgggcgca cagtatggct ggcaactggc
     7261 agggatgatg ctcattggtg cggcgctgat gcgcagcggc tggctgaaag ggcagttcag
     7321 cttacgtcac tatcgtcgta ctggttttgt gctggtggcg attggggtga ccattaacct
     7381 tcctgccatc gccctgcaat ggcagctgga ctgggcgtat cgctggtgtg ccttcttact
     7441 tcaaatgccg cgggaactga gtgcgccgtt tcaggcgatt ggctatgcgt cgctgtttta
     7501 tggcttctgg ccgcaattaa gccgctttaa gctggtactg gcaatcgcct gcgtcggacg
     7561 gatggcactg accaactatc tattgcaaac gctgatttgt accacgcttt tttaccacct
     7621 cggtttgttt atgctgtttg accgcctgga gctgctggcg tttgttattc cggtatggct
     7681 ggcgaatatc ctcttctctg ttatctggct gcgttacttc cgccaggggc cgatggaatg
     7741 gctctggcgt cagttaactt tgcgtgctgc cggaccggca atatctaaaa catcaagata
     7801 acgatctgga tcacattcgt taacaaaacg gctgtaaccg tttccattgc tgtgactcga
     7861 ttcacgaagt cctgtattca gtgctgacaa aatagccgcc agcaagcagt catttactgc
     7921 aatctcataa caggtagtga atatgatcac cattcgtgat gtagcgcgtc aggctggcgt
     7981 ctctgtggca acggtttccc gagtgctcaa taacagcacg ctggtcagtg ccgacacgcg
     8041 tgaagcagta atgaaagccg ttagtgagct ggattatcga ccaaatgcca atgcccaggc
     8101 gctggcaact caggttagcg acaccattgg cgtggtggtg atggacgttt ctgatgcgtt
     8161 tttcggcgcg ctggtaaaag cggtgggtct ggtcgcccag cagcatcaga aatacgtgct
     8221 aatcggcaat agctatcatg aagcggaaaa agagcgtcac gccattgagg tgttaattcg
     8281 ccagcgttgt aatgcgttga ttgttcactc aaaagcattg agtgacgatg aactggcaca
     8341 atttatgggt aacattcccg gtatggtgtt aatcaaccgc gttgtgccgg ggtacgccca
     8401 tcgttgcgtt tgcctggata atctcagcgg tgcccgaatg gcgacgcgca tgttgctgaa
     8461 taacggtcat caacgtattg gttatctttc ttccagtcac ggcattgaag atgacgccat
     8521 gcgtaaagca ggctggatga gtgcgttgaa agagcaggat attattccgc cggaaagctg
     8581 gattggcact ggcacgccgg acatgccggg cggtgaggcg gcgatggttg aactgctggg
     8641 gcgcaatcta caacttaccg ctgtatttgc ttataacgac aatatggccg ctggcgcact
     8701 gacagcatta aaagataatg gcattgcgat tccgttacat ctctcaatca tcggtttcga
     8761 tgatattccc atcgcccgtt acaccgaccc gcaattaacg actgtgcgtt atcccattgc
     8821 ttcaatggct aaattagcca ccgaactggc cttgcagggg gcagcaggca atattgatcc
     8881 gcgtgccagc cactgtttta tgccgacgtt agtgcgtcgc cattctgtcg caacgcgcca
     8941 gaatgcggcg gcgatcacta actcaacaaa tctggcgatg taaccgcttt caatctgtga
     9001 gtgatttcac agtatcttaa caatgtgata gctatgattg caccgtttta acgttgtaac
     9061 ccgtatgtaa cagtgaataa tcactttcac cgaggtaata gcgtcataac aacaattaaa
     9121 gccgttttct ggagcgttac cgggcatgga agaacgaatt ttaaaaagtg agcttcggcg
     9181 ttcagtaaca cttcattaac tctactgccc cgccgagcat ttatctcaag cactaccctg
     9241 cataagaaaa accggagata ccatgaataa gaaggtgtta accctatctg ctgtgatggc
     9301 cagcatgtta ttcggtgccg ctgcacacgc tgctgatact cgcattggtg taacaatcta
     9361 taagtacgac gataacttta tgtctgtagt gcgcaaggct attgagcaag atgcgaaagc
     9421 cgcgccagat gttcagctgc tgatgaatga ttctcagaat gaccagtcca agcagaacga
     9481 tcagatcgac gtattgctgg cgaaaggggt gaaggcactg gcaatcaacc tggttgaccc
     9541 ggcagctgcg ggtacggtga ttgagaaagc gcgtgggcaa aacgtgccgg tggttttctt
     9601 taacaaagaa ccatctcgta aggcgctgga tagctacgac aaagcctact acgttggcac
     9661 tgactccaaa gagtccggca ttattcaggg cgatttgatt gctaaacact gggcggcgaa
     9721 tcagggttgg gatctgaaca aagacggtca gattcagttc gtactgctga aaggtgagcc
     9781 gggccatccg gatgcagaag cacgtaccac ttacgtgatt aaagaattga acgacaaagg
     9841 catcaaaact gaacagttac agttagatac cgctatgtgg gataccgctc aggcgaaaga
     9901 taagatggac gcctggctgt ctggcccgaa cgccaacaaa atcgaagtgg ttatcgccaa
     9961 caacgatgcg atggcaatgg gcgcggtaga agcactgaaa gcacacaaca agtccagcat
    10021 tccggtgttt ggcgtcgatg cgctgccaga agcgctggcg ctggtgaaat ccggtgcact
    10081 ggcgggcacc gtactgaacg atgctaacaa ccaggcgaaa gcgacctttg atttggcgaa
    10141 aaacctggcc gatggcaaag gtgcggctga tggcaccaac tggaaaatcg acaacaaagt
    10201 ggtccgcgta ccttatgttg gcgtagataa agacaacctg gctgaattca gcaagaaata
    10261 agactgattg ggcgcagtcg tttactgcgc ccttattgta tggccaacaa ggtaaaatta
    10321 tatggtcagc tcaacgactc cgtcttccgg ggaatacttg ttggaaatga gcggtatcaa
    10381 caagtctttt cctggtgtta aggcacttga taacgttaat ttaaaagtcc ggccacattc
    10441 tatccatgca ttaatggggg aaaacggcgc aggaaaatca acattattaa aatgcctgtt
    10501 tggtatttat caaaaagact ccggcaccat tttattccag ggtaaagaga tcgatttcca
    10561 ttctgcaaaa gaagcgctgg aaaatggtat ttcgatggta caccaggagt taaacctggt
    10621 attacaacgt tcggtgatgg ataacatgtg gctggggcga tatcccacca aaggcatgtt
    10681 tgtcgatcag gacaaaatgt accgcgaaac caaagcgatt tttgatgaac tggatattga
    10741 tatcgatccg cgtgcgcgcg tcggcacatt atccgtttcg caaatgcaga tgatcgaaat
    10801 cgccaaagcg ttttcctata acgcgaaaat tgtgattatg gatgaaccga cttcttcgtt
    10861 aaccgaaaaa gaggtcaatc atctgttcac tattattcgt aaattaaaag agcgcggctg
    10921 cggtattgtt tatatctcgc ataaaatgga agaaatcttc cagttatgtg atgaagttac
    10981 cgtattgcgc gacggtcagt ggatcgctac cgaaccgctg gcagggctga cgatggacaa
    11041 gatcatcgcc atgatggttg ggcgttctct taaccagcgt ttccctgaca aagaaaacaa
    11101 gccgggcgaa gtgattcttg aagtacgtaa cctgacgtcg ctgcgccagc catcgattcg
    11161 cgatgtgtcg tttgacctgc ataaagggga gatcctcggt attgccgggc tggtgggggc
    11221 gaaacgtacc gatattgttg agacgttatt tggtattcgc gagaaatcgg ctggcaccat
    11281 tacgttgcac ggcaaaaaga tcaataacca taatgccaat gaagccataa accacggatt
    11341 tgctctggta actgaggagc gccgctcaac gggaatttat gcctatctgg atatcggttt
    11401 taactcgtta atttccaata ttcgcaacta caaaaataaa gttggtttac tggataactc
    11461 gcggatgaaa agcgataccc agtgggtgat tgattcgatg cgggtaaaaa cgccgggtca
    11521 tcggacgcaa attggttcgc tctccggtgg taatcagcaa aaggtgatta ttggtcgctg
    11581 gctactaacg caaccagaaa tattaatgct cgatgaaccg acgcgcggta ttgatgttgg
    11641 agcgaagttt gaaatttatc agttaattgc cgagctggcg aagaaaggca aggggattat
    11701 tattatctcc tccgaaatgc cagagctgtt agggataaca gaccgtattc tggtcatgag
    11761 caatggtctc gtttctggaa ttgtcgatac aaaaacaaca acgcaaaacg aaattctgcg
    11821 tcttgcgtct ttgcaccttt aagattaggg gcttcccatg agtgcgttaa ataagaaaag
    11881 ttttcttact tacctgaaag agggcggaat ttacgtcgtt cttttagttt tgctggccat
    11941 tattattttc caggatccaa catttttaag tttgttgaac ttaagtaata ttctcaccca
    12001 gtcatcggtg cgtattatta tcgcgctcgg cgtggcaggg ttaattgtca cccaggggac
    12061 tgacctttcc gcaggtcgcc aggtggggct ggcggcagta gtagcggcga cgttattgca
    12121 gtcgatggat aacgccaaca aagtgttccc ggaaatggcg acgatgccga ttgcgctggt
    12181 tattctgatt gtctgtgcca ttggtgcggt gatcggtttg attaacggcc tgattatcgc
    12241 ttatctcaac gtgacgccgt tcattaccac gctcggcacg atgatcatcg tctatggcat
    12301 caactcgctc tattacgact ttgtcggtgc gtcgccaatt tctggttttg acagtggctt
    12361 ctctaccttt gctcagggct ttgtcgcgct ggggagtttc cgtctctctt acatcacctt
    12421 ctacgcgttg attgcggtgg cgttcgtctg ggtgttgtgg aacaaaaccc gcttcggtaa
    12481 gaacattttt gccattggcg gtaacccgga agcggcaaaa gtatctggtg tcaacgtcgg
    12541 cctgaacctg ctgatgatct acgcgttgtc tggcgtgttc tatgcctttg gcgggatgtt
    12601 agaagccgga cgtatcggct ctgccaccaa caacctcggc tttatgtatg agctggatgc
    12661 tatcgcggcg tgcgtggtag gcggtgtatc gttcagcggc ggtgtgggga cggtgattgg
    12721 cgtggtgacc ggggtaatta tctttacggt cattaactat ggcctgacgt acatcggtgt
    12781 aaacccatac tggcagtaca tcatcaaagg ggcgattatt atcttcgccg tagcgctgga
    12841 ttcactgaaa tacgcgcgta agaagtgatg tgattgtggg tattgccgga tgcgtcgcat
    12901 ccggcaatcg gtgccaaatg ccggatgcgt cgctggtttt acaacgttac cgggtgttct
    12961 ttctctccct tcttaaactt cacttctccg agatcaatac aacccaccgg gcagacgtga
    13021 ccacaaagca ggcaaccaac acatttctcg gtattacaat gcggcgtgcg tgttttctcg
    13081 ctccattcca tcgcctggtg accgccgtcg taacaggaaa tataacagcg tccacagcca
    13141 acacatttat cgagattgat atggggatag acaatataac tgcggtctaa atcttccgcc
    13201 gggacaatat tgttattcgc cagacctacc atatcctgca acgaatcaaa tccctgatcg
    13261 gcgagataat gcgacaaccc gctcgccata tcttctacga tgcgatagcc gtactgcata
    13321 atgccggtgg tcacctgcaa cgtcgctgca ccgagcaata aaaactctgc cgcatcttcc
    13381 caggtttcga taccgccgat accgctgatc gggaaatcgc gcagctctgg atgggtgcgc
    13441 atttgctgaa taaagcgcag agcgatcggt tttaccgctt taccggaata tccggaaata
    13501 ctcgatttac cgttaacgat cggcatacca attttctgat taagatcgat attggtgatg
    13561 gatttaacgg tgttaatcgc cgcaatgcca tctgcgccgc cgcgcttcgc agccagcgcc
    13621 acttcgcaca tatcaccgat attcggcgtc atcttcgcca gcatcggcag cgtggaacca
    13681 cgttttactg cccgacaata tttttctacc agctccgggc tttgcccgac atcgctaccc
    13741 atcgcatgag aagtcatttg cggacaggag aagttacact cgatcatatc cgcgccagct
    13801 tcttgcacca gacgcgccag ctcttcccat tgctgctcat tttcgcccat gatcgaagcg
    13861 atcaatactt tgtccgggta atcttctttc agccgacgca gggcggccag attctcttcc
    13921 aacggatgtt cagcaatttg ctccatattt ttgaagccga taaaaccggt atcttctttc
    13981 accagatgat caaaacgcgg cgagacttcg ttggcgataa aaaaaccgat cgttttaaac
    14041 accacgccac cccagcctgt gtcgtaggct ttggcacaca tctcatagca gttgcctacc
    14101 ggcgaagaag agaggcagaa cgggttggga aacttcacgc cgcaaaaggt aatagaaaga
    14161 tctttcgtta acatgagcaa gctccctcta aataatgatg aatcgccccg gcggcttctt
    14221 tcccggtttt cactgcatag accacggttt tgtcaccctc aacaatatcg ccagcagcaa
    14281 agacttgcgg gtcgcgggtc tggtaatttt gtgtattgat ggtgttacgc tgcggctcta
    14341 actccgcaaa ggcatccagt ctggcatgct gaccgacggc gagaataatt ttatctgccg
    14401 ccatcgtcag ttcgcccggt aaccgtacat gcttaaacgt gactttattg ccttccacgg
    14461 ctactggcgt gaatccatca atgatcgaaa cgcccagctc ccgggcgctg gcaaactctt
    14521 tttcgcttgc cggaaactca tctaactctt cacgcgctac gcaagttact gcctgacagc
    14581 cgagaacttt cagcgtgctg gctacgtcca tcgcgacatc accgccgccg ataattaatg
    14641 cgctttgtgg aatgctgata tcgccttgcg cctgtcgtgc acgttgcaag aagtcgacgg
    14701 caatctcaac gtcgctatgc tcaaacagtg gtagcccgga accgctagat aaccccacgg
    14761 tgaccagtac cgcgcggttt tccgctttca gctgctccag ggtgagtgtt ttaccgattt
    14821 cgttattgca cttaatggtc acgcccattt tttcaatacg ggcgatttct gcatccagta
    14881 ctgattgcgg taaccggaat tgcggaatgc cgttacgcaa ccagccaccg ggttgtgctt
    14941 ctttctcata gatcgtgacg tcataaccct ggtttgtcag tgtcacactg gcctgcaatc
    15001 cggcaggacc cgcgccaata atggcgactt tgccgagcgt tttagtaccg ggctgataaa
    15061 tttccatccc agtttgttgt tcaaaatcag taacaaaacg ctgtaagcga ccgatatcaa
    15121 tgggagtatc aacaccggca cgggtacaac cgctttggca taatttctcc gtcgggcaca
    15181 ctctggcaca aacggcaccg agggcattat tttcacgaat tgtctcggca gcgcctttaa
    15241 aattacgaaa gtagattgag cgaataaatt tccccggatc ggtctgtgcc gggcaagcct
    15301 gactacaggg agcgtcgtga cataataagc agcgagaggc ttctttaatc gccagtaacg
    15361 gtgtaaaagc cggagtgagt tcatccagat aattttgttg cggcatgtga tcttccttaa
    15421 tgctatgagt aattaaggcg aacgctaaat atactcgtca tacttcaagt tgcatgtgct
    15481 gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga ctcactcact
    15541 tgtcgccttc ctgcaacttg aattatttag agtataaata ttgcgtgctg atattaaggc
    15601 tatgcgcgtg gggaaattta aactgtgaaa ggattcacaa atagctgcgg ttcagaatgt
    15661 tatatccgca gcttattgag tgaagtaata attattctca ataattaaca gaaaagagaa
    15721 aatattaacg atagaaaata agataaacaa tcatgccgat agataaaatt ccgaagccga
    15781 tatatgccac catataagcg aagaagagtc cgggattttt tgctcgccag acatacaccg
    15841 gttccggtgc atttttaacc cgtttatcaa tcgcgcctgc gcgccagcct ttccatgctt
    15901 cgcggaaaca aaatatcgga atgaggaaaa aaacggcaac gacaaaaaaa tgctgtacat
    15961 ccataactac tttccttgtt tcttttgtaa ttcaagtaac tgttcgggcg tgacggcggg
    16021 atacccctgc gcatcttccg gcacctggtg catagccgga atagggacca gcggccctaa
    16081 aaaacgcggt tcacgtttaa aaatataaag gtcagccagc gcaccgaaac gggcggcaaa
    16141 ttcgcgaata cgtactgaca gcatatcctt cggtgacggt acggcataac actgagcctg
    16201 aatccccata tgcagcgcaa taaataatgc ccgctcacag tggaaacgtt gggtgataat
    16261 aatgaaatca ttagtatcga aaactttgcg tgtacgcacg atggaatcca gcgtacgaaa
    16321 gcctgcgtaa tcgagaacaa tatctgatgg gtcgacacca gcagcgatta aatctttgcg
    16381 catggtcatc ggctcattat aactttgcaa tgcgttatcg ccgctcagta atagataatt
    16441 taccttaccg ctgttatagg cattaatcgc tccttgaata cggtagcgat aatactgatt
    16501 aattacgcca gtacgataat attttgctgt gccgagcacc acaccgacct ggcggtaggg
    16561 gagatcctgc aattcgtcgt agatataagg cgcggttttc cagctcatcc agcgatcgag
    16621 gccgagcaca gtcaacagca gcaagccgat caggactaac aggctgagga acacgcgctt
    16681 taacatgcag gagactcgat ctaaggaaaa tttccatcag gctactttac ccgctggttt
    16741 tgcgcaagaa acggcaaact gtttgccggg attttcgaca ccaggagagg gctggcaagc
    16801 cggacaaggc gttcacgccg catccggcac caggcttaag cgagaagcac gcggtcgata
    16861 ttgtgacagc cgagagcttt cagtgttgcg gaggtggcat cccactgaat caacggcgca
    16921 tcggcttttt ctgccagaac cgcgcgctgg atatccgggt aatcataacc cttgagattc
    16981 aatagaatta acgctccctg caacggtggc agagtcgggt tgaatgcggc gttttcagcg
    17041 taactgccgc tgaaaatacg accgtcttta cattccagcg cgacaccgct tggcgactta
    17101 ctgtaaggca tgtgcgaacg gtttgccgcc gcaatcgctg cctgagaaag cgcatcaccc
    17161 gtcagcgcat agccgtgatc ctgctcgtcc atcagcagcg ttttaatctc cagatctttc
    17221 ggcccaaagg catccggtag atagtcacgc agcgcgtgtg cctcgcggcc cggcagatga
    17281 atacgcagat ccagaccgct gtttagttca ttcataaact gacggcagtg accacaaggc
    17341 gtgtagttaa cggtgatggc tgcaagcgct ttttcaccac tcaaccaggc gtggctgatc
    17401 gcgctttgtt cggcatgaac ggtttgctgc attgtcgcac caataaattc catattggca
    17461 ccaaaatacc aggttccgct cacaccgcgc gcaattgcgc caacattaaa attcgacaat
    17521 ggcgtacgcg cacaggccgc cgccagcgga agtagtgcga atgccagcgc gtcttcgtcc
    17581 agccccgttg cgctctttag cgatgagact tgctccccgg tcaacaaagc ggggaagtac
    17641 ttgtctgcca gaataggttc cagtgcagat tgcaagttat ccgcaagttg ggcaaaagcg
    17701 gtttgaaaac gtggatgcat ggcgttgcct cataatcatg taatgggata acagtttacg
    17761 aacccgttat ggctttatat gtgatctgaa tctcattaat tatgcaacgc gtgaaactta
    17821 catcaaaaat gcgcgacgca tcgcaaattt tagcccatta ctgccagaat aatcgggaaa
    17881 aggaacggtg cgatcagcga agtaataatc ccgcataaca ccagtgccag cgaactaaac
    17941 gcaccttcct gataatccag ctcggcgcag cgcgccgtac cgagggcgtg cgaggcagtc
    18001 cccattgcca gaccgcgcgc agctttggta cggatacgca tcgcattaag caatgtatgg
    18061 ccaaacacag cgccgaggat gccgacgaaa atcacgcaaa ctgcgctgat tgccggaata
    18121 ccgccaatac tgccgccaac cgccattgca ataggcgtgg tgactgattt cggcaggatt
    18181 gacgcggcga tttccggtga agcgcccatc aacaatgcca cggaagtccc cgtcaccatc
    18241 gcaaccacgc tgccgataaa acagatggtg atgatcgatt tccagcgcgc gcggatctgg
    18301 tgtagctgct catataaagg ataggccagc gcaacgaccg ccggttgcag tagatcgttt
    18361 agcacttcgc tacctttaaa gtagctgtcg taagagatgc cggtcagcat taaaaaagga
    18421 atgatcacca ccattgctac cagcaacggg ttaagcaagg gaaacttata ccgtgccgcc
    18481 agtttgcggg cggcaaaaaa gacaatcaat gtcagcggta atgaccacca gatattcgcc
    18541 atcattcttc tgatcctttc tgacctacga ctttacgttc accatgcacc agttgcgaac
    18601 tccagctcac caccagaaaa accaccagcg tactgactgc acaggaaacc actaccgggc
    18661 caaactgtgc gcggagcaaa tcaaaatatt gcatgacgcc tacgccaatc ggcacaaaca
    18721 atagcgccat atagcgaatc agtacgtagc accccggatt gacccatttc gccggaagaa
    18781 tttgcaaggc cagcaggaca aacaggatca gcatcccgat gatgctgccc ggaatggtta
    18841 ccggtagcag tgaagcaatg aaaatgcctg catacaggca ggcataaatc aggacaaaag
    18901 cacgtaaata ttgccagata atgttcagtg tcttactcat aatcaacacc ccagatgaga
    18961 tggattcatc atacaattaa ttcgtaaaac gtgctaccga tcacattaag aaatcagcgc
    19021 ttattttttc gctcccgggc attacgcaat gagcagcgaa ctcaggcata catcaagaat
    19081 agagagggat tgcagtttgc agcaatccct ctttgcagac ctttaatccg ttgactatag
    19141 gcgctaacat agcgcctcat ttttttgcgg gtgatgatat gcgtgtgtta ctggcaccga
    19201 tggagggagt gcttgactct ctggtgcgtg aattgctgac cgaagttaac gactacgatc
    19261 tgtgcatcac cgagtttgtc cgcgtggtgg atcaactgct gccaataaaa gtctttcatc
    19321 gcatttgccc tgagctacaa aatgccagcc ggacaccttc cggtacgctg gtgcgcgtgc
    19381 agttgttagg tcagttccca caatggctgg cagagaacgc cgcccgtgcg gtggagttag
    19441 gttcctgggg cgtggatctc aattgcggct gcccgtcgaa aacggttaac ggtagcggcg
    19501 gtggggcgac gttactcaaa gatcctgaac tcatctacca gggggcaaaa gcgatgcgtg
    19561 aagctgtacc agcgcatttg cccgtcagcg taaaagtgcg tctgggctgg gacagcggtg
    19621 agaagaaatt tgaaatcgcc gacgcggtgc aacaggctgg cgctacggag ctggtggtgc
    19681 atgggcggac gaaagagcag ggttaccgcg cggagcatat tgactggcag gcgattggcg
    19741 atattcgcca gcggctgaat attccggtga ttgccaacgg tgaaatctgg gactggcaga
    19801 gcgcgcaaca atgcatggcg atcagcggct gcgacgcagt gatgattggt cgcggggcgc
    19861 tcaatattcc caacctgagc cgtgtggtaa aatataacga accgcgaatg ccgtggccgg
    19921 aggtggttgc tttgctgcaa aaatataccc gtctggaaaa gcagggcgat accgggttat
    19981 atcacgtagc gcggattaaa cagtggttga gttatttgcg taaagaatac gatgaagcaa
    20041 cggaattatt tcagcatgtt cgggtgttga ataattccca tgatattgca agggctattc
    20101 aggcaattga tatcgagaaa ctctaagaaa ccatagtgat gttttttgac aatgtgttag
    20161 ctctttatca ataggccgca cggttg
//
LOCUS       DAPOZO010000087        19954 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPOZO010000087 DAPOZO010000000
VERSION     DAPOZO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.087"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..441)
                     /gene="alaE"
                     /locus_tag="ROI21_003497"
     CDS             complement(<1..441)
                     /gene="alaE"
                     /locus_tag="ROI21_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDX5112379.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQV"
     gene            1108..1512
                     /gene="stpA"
                     /locus_tag="ROI21_003498"
     CDS             1108..1512
                     /gene="stpA"
                     /locus_tag="ROI21_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDX5112380.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(1559..2083)
                     /gene="ygaP"
                     /locus_tag="ROI21_003499"
     CDS             complement(1559..2083)
                     /gene="ygaP"
                     /locus_tag="ROI21_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDX5112381.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(2093..2392)
                     /locus_tag="ROI21_003500"
     CDS             complement(2093..2392)
                     /locus_tag="ROI21_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX5112382.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2575..2733
                     /locus_tag="ROI21_003501"
     CDS             2575..2733
                     /locus_tag="ROI21_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDX5112383.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2817..3266
                     /gene="kbp"
                     /locus_tag="ROI21_003502"
     CDS             2817..3266
                     /gene="kbp"
                     /locus_tag="ROI21_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDX5112384.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTVTPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(3267..3929)
                     /gene="csiR"
                     /locus_tag="ROI21_003503"
     CDS             complement(3267..3929)
                     /gene="csiR"
                     /locus_tag="ROI21_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDX5112385.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKRDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(3950..5350)
                     /gene="gabP"
                     /locus_tag="ROI21_003504"
     CDS             complement(3950..5350)
                     /gene="gabP"
                     /locus_tag="ROI21_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDX5112386.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            complement(5588..6868)
                     /gene="gabT"
                     /locus_tag="ROI21_003505"
     CDS             complement(5588..6868)
                     /gene="gabT"
                     /locus_tag="ROI21_003505"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDX5112387.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHTEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            complement(6882..8330)
                     /gene="gabD"
                     /locus_tag="ROI21_003506"
     CDS             complement(6882..8330)
                     /gene="gabD"
                     /locus_tag="ROI21_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDX5112388.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            complement(8353..9621)
                     /gene="lhgO"
                     /locus_tag="ROI21_003507"
     CDS             complement(8353..9621)
                     /gene="lhgO"
                     /locus_tag="ROI21_003507"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDX5112389.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIHNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLSLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            complement(9641..10618)
                     /gene="csiD"
                     /locus_tag="ROI21_003508"
     CDS             complement(9641..10618)
                     /gene="csiD"
                     /locus_tag="ROI21_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDX5112390.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLTPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSQGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATNHYQTHQ"
     gene            complement(10955..13207)
                     /locus_tag="ROI21_003509"
     CDS             complement(10955..13207)
                     /locus_tag="ROI21_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112391.1"
                     /translation="MFSIKPGPRNLPIGNPTLLSWNITDGDLNSKLNTLEYLNCITNI
                     INSCGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLFPDTTYRELNKAAQRSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPTGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRKNISEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIIDPVTKERDQIHPEDIHLTAKDFEASKDNISKDEWEN
                     LHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYQKKAVMNYMEYLVTCPLDEQQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLQTLASKPGSTISSIPSEKRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNDDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKAFTLRGGTLNINIPGRSALLLGK
                     TGEPPNYLYL"
     gene            13767..13842
                     /locus_tag="ROI21_003510"
     tRNA            13767..13842
                     /locus_tag="ROI21_003510"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13800..13802,aa:Ile,seq:cat)
     gene            14599..16053
                     /locus_tag="ROI21_003511"
     CDS             14599..16053
                     /locus_tag="ROI21_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HDX5112392.1"
                     /translation="MLVSKSNEINTSTFINSGSCNERKSSKSMELLAYSIIKLICKEA
                     ASGTYNGALEILQKMMSECKYHEGNAFVIMGAGEQLKRIKYDANENKLKVYNVHFDNN
                     QELVADGEPDVVFLKKTVWEDLLVKLKLENKENAVSEINLSSNKNNVDQFIECAKRNE
                     QTLFGNIRKSDFHVASLQPGRTRSVISETPPNDCMESHNLYENHTDTVSTVTKNSQQV
                     KGHYGDKLKEMQLFLNQMSNALQQDSSLLESKEHTIDIQEKTNKFVQHFQRVLFDKNG
                     RSSEFLLNFYECCYKFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNVGFNFKLFPE
                     VKLSGGELETVFKYKNGNFVWEIARIKITLPKEEGGLYNLRGLDFKGCFFSGQNFSNY
                     DIQYVNWGTSLFDLDTPCIFNAPAYNKSNEKSLERVSENGLSGVLSDRNKKIKLITGV
                     APFDDILFMDDDFDDNSPDDGPVENSPVVNSPLV"
     gene            16441..16650
                     /locus_tag="ROI21_003512"
     CDS             16441..16650
                     /locus_tag="ROI21_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDX5112393.1"
                     /translation="MLRTLSAGYALLVCKLNRLGRSMWHLVVLLEELCKRGINFRALA
                     QSIFPQQWGDECCKSKTICDLKVIV"
     gene            16804..16923
                     /locus_tag="ROI21_003513"
     CDS             16804..16923
                     /locus_tag="ROI21_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDX5112394.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            16988..>19954
                     /locus_tag="ROI21_003514"
     CDS             16988..>19954
                     /locus_tag="ROI21_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDX5112395.1"
                     /translation="MNRTSPYYFRRSVLSLLISALIYAQPGMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGRQEVYGGISNSSIIETGGEQLVSIHADINGQANNTTI
                     NGGRQSIEYGGISTGTIIESGNQYVYKGGTSNDTTIKGGTSRIEGGTANGTIIDGGGQ
                     SVSTQGHVDGTTINKSGYQDITQGSLATNTTINGGRQYVEQSTVETTAIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIVDNTSSSDVIEVYSGGVLDVRGGT
                     ATNITQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVAENVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISSGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVMAKTSAKNTTIDAGGK
                     LIVQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDA
                     FYIRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIY
                     VSATSEKANIKGGKQTVYGLATEANIESGEQIVDGGSTDKTHINGGTQTVQNYGKAIN
                     TDIVSGLQQIMVNGTAEGSIINGGSQVVNEGGLAENSVLNEGGTLDVREKGSATGIQQ
                     SSQGALVATTRATRVTGTRADGVAFSIEQDAANNILLADGGVLTVESDTTSAKTQVNA
                     GGREIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAVKTTINEGGTL
                     TVNDNGKATDIIQNSGAALQTSTANGIEISGTHQYGTFSIAGNLATNVLLENGGNLLV
                     LAGTEARDSTVGNGGAMQNLGQDFATKVNSGGQYTLGRSKDEFQALARAEDLLIAGGT
                     AIVYAGTLADASVSGAT"
BASE COUNT         5440 a         4560 c         4786 g         5168 t
ORIGIN      
        1 tacctgctgg taacggctga ctttaaacag tcggcggcaa taatcgagga agtagccata
       61 aaccgccccc atcaacatcg aaacaacgat gtttgaactg accgccgcca taatctgatg
      121 ccagtctgcg cccaccacta acaagatcgc cacatacacc ggtgactgga acgtcacata
      181 agccatgata tccgccagat tttttatcca gcccgacggg ctaactttgc gtgctgcgcg
      241 cataaacaga tcgcggtaca taccgtatgg ccatgcaatt aagatgttca ccggaatcgc
      301 taccaatctg gaataaaaag actgttcgaa gctcattccg gagaggaaaa cttcaataca
      361 catgttcacg acagaacagt aaacaaccat cgcgaacgta tctgcaactg catgacgcaa
      421 gcgtgactgc ggtgagaaca tgctaatgct ccttaataaa agcgtaatgg gatgtgaatg
      481 gagatgaaag cagtggatct cattgccatc tgcattttcg catagcgtat atcgctggat
      541 atgactaatc aattagtgaa aaatttttat ttaatctgtt tttgtagata aaatattccg
      601 taacggcgcg ttttttgtac attgtggatt tttacatgcc aatatgattt atttttaatt
      661 aaatcaattg gatatgtgtt tacctttatt ttcactcatg aaggtttggg gggaggtaac
      721 gctataagcg tagagaattc ggtgtatgtc gtgaccgggt cattttcagc attttttatg
      781 ccagataatg tacattgaca gcagaaagca ccagtgatag atgttttctt ttattctgtt
      841 gagttgcatt tttattcact ttggttttgt caggcttgcg gaattagcga gcagagagcg
      901 ccgctctgct cacaaataac ctaaattact catcaaaatg gctaatatat aatcttgaaa
      961 ttattctgag agtttcagaa aataacagct gaaataatct cgcgcaggac tgtaaataga
     1021 ttaaattttg tggaaatata ataagtgatc gcttacacta cgcgacgaaa tacttttttg
     1081 ttttggcgtt aaaaggtttt ctttattatg tccgtaatgt tacaaagttt aaataacatt
     1141 cgcactctcc gtgcgatggc tcgcgaattc tccattgacg ttcttgaaga aatgctcgaa
     1201 aaattcaggg ttgtcactaa agaaagacgt gaagaagaag aacagcagca gcgtgaactg
     1261 gcagagcgcc aggaaaaaat tagtacctgg ctggagctga tgaaagctga cggaattaac
     1321 ccggaagagt tattgggtaa tagctctgct gctgcaccac gcgctggtaa aaaacgccag
     1381 ccgcgtccgg caaaatataa attcaccgat gttaacggtg aaactaaaac ctggaccggt
     1441 cagggccgta caccgaagcc aatcgctcag gcgctggcag aaggtaaatc tctcgacgat
     1501 ttcctgatct aattactgag gccggatgtc gctgctatgg cgcgtccggc gtcgcttctc
     1561 aagctcgttg gttccaaggc atcttatcta acaaccttgc catcccacaa aaaccgctaa
     1621 ttcctgcaaa cagtaacccg gcaccaacaa agccgcttaa taagaagaaa ccgctattta
     1681 cggtataacc cagtacaacg ccgattaata ttaaaccacc tgcggcgatc tgcacctggc
     1741 gcattaacgg caagggttga gatttattta ccgctactgg caatcctgct tttttccagc
     1801 catcaatccc atcttcgagt aaaaagattt ctgcgggggc ggcaatcgct gctaatttat
     1861 cggcgttatt actggtgcgt ttacctgcct ggcagtggaa tataatttgc tcgcgacgta
     1921 atttagccgg aagacctgac tgttcgagca cggataatgg agccagatct gcttcaggaa
     1981 tatgttcacg aagatattca tcagcgtcac gaatatcgat taactttgcg ccgcgtgcga
     2041 ttaattcttg tgcatcatgc ggcgaaatgg ttgtcaaagc catagtgact ccttacggac
     2101 aatagacatt tttaagggtg gcgataatgg catttaccgc ctcattttta atggaatata
     2161 gaatgcgttg ggcatcccgt tggctgtcga taagcccttc gtcccgcata cgagcgagat
     2221 gctgtgatgt tgcagaggca ctcagtccgg taatgcgcgt cagctctccc gcgctcgtgc
     2281 cgggggaacc gctaagcatg cacagaatca gcaaccgttt agggtggctc attgctttca
     2341 ataaggccgc tgcctgttcg gcactggcct gtaattgcgc gagttcagtc atagttattt
     2401 aagtgttttc taaattaagt aaactctaaa ctaaaaatgc aacatatacc agcatcagca
     2461 gcgtaaatga gagtaaaagc gtaagctgaa actggcaggc tccgctaaaa ttactacgct
     2521 taagagataa aatctctttt taaacaatga gtaattttct tatagggagt acatatgggt
     2581 ttctggagaa tcgtcatcac catcattctg ccgccgctcg gcgtgctgct cggtaaaggg
     2641 ttcggttggg cgttcattat taatattctg ctgacactgc ttgggtatat ccccggtttg
     2701 attcacgcgt tctgggtgca aacccgcgat taaacctgtg ttcacccccg cggcgttaac
     2761 gcgccgcgtt cctctgctac actttctgag tgtttccgtt aacgaatgag gtagctatgg
     2821 gtctgttcaa ttttgtgaaa gatgccggag aaaaactctg ggacgcggtt acaggtcagc
     2881 acgataaaga cgatcaggcg aagaaggtgc aggagcatct gaacaaaacc ggtatacctg
     2941 atgccgataa agtgaacatt caaatcgccg acggcaaagc gacggtcacg ggtgatggcc
     3001 tgagtcagga ggcaaaggag aaaatccttg ttgcggtggg aaatatttcc ggtattgcca
     3061 gtgtcgatga tcaggtgaaa acggtgacac cagccactgc cagccagttt tataccgtta
     3121 agtctggcga cactttgagt gccatttcca aacaggtcta cggtaacgcc aatctgtaca
     3181 ataaaatctt cgaagcgaat aaaccgatgc tcaaaagccc ggataaaatt tacccggggc
     3241 aagtgttgcg tattcctgaa gagtagttaa ttgccagcca ttgcctgctg gataatggga
     3301 attggcgtca gtaaatgctg gcgcattaac tcactggcgc gcgcggtatc tcgcgccagt
     3361 accgccgcag tcagggtctg gtgctgatcg cgtttatctt ccagcatttc caccgaaagc
     3421 actgttcgcc gcagccagat aaatcgatag cgcgccgcca gatcaaacaa ccgttcacgc
     3481 atttgcagca aatagtgaga accacagcct gccacaattg cagtatgaaa tgcctgatga
     3541 cgcagatccc actcatcaag cattttctca ctggcgtcac aggcctccag cttactgagc
     3601 agatgcgctt ttgcgagaac gtctgcctcc cattcatcgc caccgcgggc aatcgccaga
     3661 ctgaccagca tcgcttccat attggcgcgg gcgtcgaaaa tatcgagcag ctcctgctct
     3721 gacatagacg ccacccgata ccctttttga ttcaccaccg tgaccagtcg ttctgctacc
     3781 agttgcgaaa gcgcttcccg caacggccca acgccaagtg cataacgcga tgtcagcaaa
     3841 ctcatccgta atttttcatc cggttgaaag tttccgcgaa taatatcgtt cttcagccag
     3901 cgatagccat ccagagacgt aatggtcatg gtgtgactcc tgatcattat tagcgcgtat
     3961 tatgaacggg tgttttttgc cacaatacca gctttttcca gcgagccata attggcacgg
     4021 tacagataat ccctatcgct aataagccgg tggagatcac ttctaactgt tgcgccgggc
     4081 ggaatagcat cactaccaac acaaaggtaa taaagccgat gaccagccag gtgagccacg
     4141 gataaagcca catccgcagg cgaatttcgc ttccttctgc tcgcagaatt ttgcgcatcc
     4201 gcaattgtga aacggcgatg actaaataaa ccagcagggc gatagcgccg gagctgtcga
     4261 tcagaaattt aaacactttc gcaggcgcgt aataattcac caccaccgtt aaaaatgccg
     4321 caccggtcga gagtaatacc gccacatacg gggttttact gcggttgatt ttacccatta
     4381 ccgcgggagc atcaccgcga cggcttaagg agtagagcat tctggaagcg gtatacagcg
     4441 ccgagttcag acagctggtt acggaaagta atatcacgca gtccatgatt aatttcgcat
     4501 ggggaatatt gagtaattcc agaaccgagc gataagatcc aacggctttc agccccggca
     4561 tattccacgg tattaatgcc acgacgacaa aaatagagca caaatagaag atagaaatac
     4621 gccagataac cgagttggta gcgcggacaa tatgtttttc cggcgtgtcg gattccgcgg
     4681 cggcaatggt gacaatttct gcgcccataa acgagaacat ggtgatcaac atcgcgctta
     4741 ataccgcgcc gaagccgttg ggcataaagc cgccgctatc ccacaatctt gagatcccgc
     4801 tcacttcggc ataagggtaa aaaccgctaa ttgcgactgc accaaggaaa ataaaggcca
     4861 ggatagcgat gactttgcac agcgccagcc agaactcaaa ttcgccgtag tttttaacgc
     4921 ttaataaatt actgccagtt aaggcgaggg taatgacgag ggaaaataac cagatgggaa
     4981 tgcctggaac ccacgagtgc aggatcatgg cggcgatgtt ggcttccagc gggataacca
     5041 gtacccaaaa ccaccagtac aaccagccga tggtatagcc cgcccaacga ccaatagctt
     5101 tatctgcata ggtggaaaac gaacccgtgt cgggcgtggc aaccgccatt tccgccaaca
     5161 tccgcataat cataaccacc agtaatccgg cgaacagata ggccagtaat accgccgggc
     5221 ccgcttcggc gatggcgacg ctggaaccga caaacagact tgcgccgata acaccggcaa
     5281 tagacaacat ggtgacgtgg cgtgacttca gcccgccgcc taactcatgt ggttgcgatg
     5341 attgccccat cctgaatcct ctcgaaagtg tgtcacttgg gagagcgccc cgacatttcc
     5401 cgcgtcgggt cacgcgtgta attgttattg gttcttttta ccggagagcg gctccgcctt
     5461 atccggccga catagattta ttcctgttca cgtacagatt gtcggatgca gcgtaaacgc
     5521 tttatccgac ctggattcgc gtaggccgga caagacgcgc agcgtcgcct ccggcatggg
     5581 tacggatcta ctgcttcgcc tctgcaaaac actggctgat gatctccaga ccctgacgga
     5641 tctgagcgtc ttcaatggtg agcggtacaa ggatgcgcag cacgttgtaa tacgggccgc
     5701 aggagagaag aatcaggccc ttatcgcggg cgcgcgccac gatctcggcg gtgagttttg
     5761 cgtccggttt gctgtgatcg ccatcttcaa acagctcgat ggcgatcatc gctcccagcc
     5821 cgcgtacgtc gccgatctca gtatgttttt cggctatcgc cagcaatccg tctttcagtt
     5881 tctgccccag atcgttggct ttttgtagca gattttcctg ctcaaacacc ttcaacactt
     5941 ccagcgcagc cacgcaggca atcgggttac ccgcataggt gccgcccaga ccgcctggag
     6001 cgacggcatc catcacttcc gcgcgcccgg tgacgcccgc cagcgggaag ccgcccgcga
     6061 tcgatttcgc aaaggtggta agatccggcg caacgcccat ctgctccatc gcaaacagcg
     6121 tgccggtacg ccccgcgccg ctctgcactt catcggcaat cagcatgatc ccgtgctcgt
     6181 cacacagcgc gcgtaaacgc tgcataaagg ctggcgtcgc ggcgtagaaa ccgccttcgc
     6241 cctgaaccgg ctcaatcacg atggcggcga tatcttccgg cgcagcgtca tttttaaaga
     6301 tccggtggat gctggcgata gcgtcatctt cactgatgcc gtgcagcgga caaggataaa
     6361 gcgcgcgata aacgtgccca ggcatcagtc ccatgcccgc agagtacgga ttcaccttgc
     6421 cggtcagcgc cagcgtgtaa tgcgtgcgcc cgtgatacgc gccgctaaag gcgatggtac
     6481 cgctacgttt ggtggcggca cgggcgattt tcaccgcgtt ttccaccgct tcggaaccgg
     6541 tcgtaaccag cagcgttttc ttggcaaaat cgcccggcac cttctgattc ataatctcgc
     6601 acagctccag atacggctcg taggccagca cctggaagca ggtgtgcgac agttttttca
     6661 actgcgcttc cactgccgca accactttcg gatgcaggtg cccggtattg agcaccgcaa
     6721 tcccgcccgc gaaatcaaga tactcacggc cttcaacgtc ccacacccgg cagttttccg
     6781 cgcggtcagc gaaaatcggg tgaatttgcc caacgccacg gggaatcgcc tgactgcggc
     6841 gctgcattaa ctctttattg ctgctcattc gcgttctcca gttaaagacc gatgcacata
     6901 tatttgattt ctaagtaatc ttcgatgcca tacttcgaac cttcacgacc cagacccgag
     6961 gctttaatgc cgccgaacgg ggccacttca ttggaaataa tgccggtatt gatgccgacg
     7021 atgccgtact ccagcgcttc gcccacgcgg aagacgcggc ttaaatcacg ggcgtagaaa
     7081 taggcggcaa gaccaaactc ggtgtcattg gcttgcgcaa tcacatcggc ttcatcttta
     7141 aaacggaaca gtggggcgag ggggccgaac gtctcttctt tcgacacttt ggcgttggct
     7201 ggaacgtcca ccagaatggt cggctggaag aagttgccgc cgcgttcatc cgctttaccg
     7261 ccgcaaacca cacgcgcacc tttctccagc gcatcggcaa tatgctcttc cacttttgct
     7321 accgcttttt catcaatcag cggcccgatg gtgacgccat tctccagccc gtcgccgatg
     7381 tgcagtttgc tcaccgcctg ctgtaatttt tcggcaaagc ggtcatacac gccgtcctgc
     7441 acgtataaac ggttggcgca gacgcaggtt tgcccggcgt tgcggaattt cgaggccagt
     7501 gcgccttcca cggctttgtc gaggtcggca tcgtcaaaga cgataaacgg cgcgttgccg
     7561 cccagctcca gcgacacttt cttgatgtct ttcgcgcact gttccattaa ctggcggcca
     7621 atttcggtcg agccggtaaa cgacagcttg cgcaccagcg gattgctggt cagttcgtta
     7681 ccgaccgcgc ccgccgaacc ggtgaccacg ttaaatacgc cagccggaac gcctgcgcga
     7741 atcgccagct ccgccagcgc cagcgcagag aacggcgtct gactggcggg cttcagcacc
     7801 atggtgcagc ctgccgccag cgccggaccg gctttgcggg taatcatcgc cgccgggaag
     7861 ttccacggcg tgatagccgc ggtgacgcca atcggctgct tgataacaat caggcgttta
     7921 tcggcctgat gaccaggaat ggtgtcgcca taaatgcgtt tgccttcttc ggcaaaccac
     7981 tcaataaagg aggcggcgta gctaatttcg cctttcgctt cagccagcgg tttgccctgt
     8041 tctagggtca tcaggcgcgc taaatcgtcc tgatgctcca tcatcaaatt gaaccagttg
     8101 cgcagaatgg tggcgcgttc tttggcggtg agcgcacgcc aggcgggcag agcgcggttg
     8161 gcggcgtcga tagcggcgcg ggtttcatca gcgcccattt tgggcacgct acccagcttg
     8221 tcgccgttcg ccggattggt gacgtcgatg acctcgccat tgttggcgtc cagccattcc
     8281 ccgttaatca acgcctgctg gcggaataag ttactgtcgt taagtttcat tgttacgtcc
     8341 tgttttcaaa ggttattgat taaatgcggc gtgtaaggca tccacactac gtgccgctcg
     8401 cagcgtgcgt ccggggttac tctggcttgc caacagcgtt tgtaccttgc tgacaatatg
     8461 cgcaccaata ggaattgctg atgtcgctgc cggggagggc gcattgcagg tgtggatcgt
     8521 gcgcggggtg gtgacaaaca gaaaatcgtc aatcagcttg ccgtccggcg ataccgcctg
     8581 cgcccgcaca ccggcgggcc agggctggag atcgcttaac gaaagccggg gacaatactt
     8641 ttgcaccagc cgcaaatagc cgcttttgca cagcgagttt ttcatctcgc ccagtcctga
     8701 gcgtagatgg ttttgcagca cccggcgaat ccccgacgag cctaaaatct ccagcgtgtc
     8761 actaaacgag aaatcgcgct tgcgatagcc ttcgcgttta aaagccagca ccgcgtttgg
     8821 cccgacggtc acgctgccat cgatcatgcg ggtgagatgc acgcccaaaa atggcatcgc
     8881 ggggtcggga atggggtaaa tcaggtggtt aacaatctgg ttatgctccg gcgcaaggcg
     8941 gaaatactcg ccacggaacg ggcagataat aaagcccggt tcgaggctga gcattttcac
     9001 cagccggtca gccatcagcc cggaacagct aatcagcgtc gatgcttcat attcaccgcc
     9061 ctgacgggta cgtatcacca cgccgttttt atgctcactg agggcgctga cttcggcgtt
     9121 atgaataatc tcgccgcctc tggcctggaa gatttttgcc atcgccgccg tcacttcgcg
     9181 atagctgaca atgccgctgg acggcacaaa aatgccgccg agcccggtga tattcggttc
     9241 gcgctcgcgc agctcctcgg cgtttaacca ctcgcgctcg ataccgttcg ccgccgtgcg
     9301 ttcccacaac gcgcgcatcc gttccatttc gagttcagag gtggcgacca gcatcttgcc
     9361 gcagttgtca tagcgaatgc cgttttgatc gcaaaaggct ttggtggcgc ggttccccgc
     9421 caggcaaaac tgcgccttta ggctgccggg cgtgtaatag accccggcat ggatcacgcc
     9481 gctgttgtga cccgtctggt gacaggccgg gccggactct ttttccagca acgcaatgcg
     9541 ggcatccgga tagacatcaa tcagttgcat ggcggtcgac atgccgatga tgccgccgcc
     9601 aataatcaca aaatcataca tccgctcaat tcctttgcgc ttactgatgc gtctggtagt
     9661 ggttagtggc gtaagcgaaa tagccacgct gacgcatcag ttcacggcgc agatccgggt
     9721 gcggagtaaa gcggtcgcga ccgtgcagcc agaacaggtt gtttatcaac aggaatttgc
     9781 caacgggaac gggtacagaa agaatgcctt ggctggtttc aatggcatcg gaaagctcgc
     9841 tcaaccacac gccttcttca aaatcttttg gctggacgaa ctggtcgata taacgcatca
     9901 ccgggcgacc ctgttgatca acgtcgaaca ctggatggaa aacatctttg ctgacgtttt
     9961 tgctcggcgg cgcggcaaag cgcatcgggc gacgcgccag cgggtggcgg aaatagtggt
    10021 ccagatgttc ccagtcatcg agatgcagca gcaacgaatt tccaccctgc atgttctgct
    10081 cgtcgatttt catcatcagc acatagtcgg tgatctcttc gacgtaggtg ccgtcgttgt
    10141 gcagctccat tacgcggtgc ggctgacgca gatagctgtc tgagttatcg acatttttca
    10201 ccacgaatcg cgcgtaatac tgaccgctca tcgcatcgaa atttgagcga ccaatcagat
    10261 gcgctaccgc cgttgccagc ttcaccatct catccgcttg cttcacatca tcgacaccca
    10321 ccgcattgat caacagcgca ccttcagcac ggtttagcag ggtcttcaac agcaatggtt
    10381 gcagctggtt cgcacacaaa tcgtcaagaa ttttgcctac ccgaaaacgc agaaaagatt
    10441 tgtactccag cgcctgcacg ggccattcgg caacctgttc gagaaactgt ttggtcgtct
    10501 gttcggtgaa ggtgagttcc agcaggcgcg gggattgcgc cgacggggtg agggtgaatc
    10561 cgctatagtc ctggcctgaa tcgacagcgt tgttttgtac ggcggtcagt gcattcatca
    10621 gaagcgatcc tcttatgaga tgtagggtga catggcgatg ctcatttcgt agccataatc
    10681 taaaaatatc tacatttctg aaaaatgcgc acaaatgcgc catattgttt tcttattgtg
    10741 atcaaaagca acaaattggt aacatttaac gtagggtatt aaggcaaagt gagacaggaa
    10801 atgaataact aaagcaacag gttagggggg gattaccatc agagaacaga ttatctggtc
    10861 gataaaaaac ggtaaatggc ttcactgcaa aatgaccgtt agtaaactaa acatattatc
    10921 tgccccgttt tagcggggca gatataacat taaattacaa atagagatag ttcggcggtt
    10981 ctccagtttt ccctaaaaga agagcgctac gcccaggaat attgatattt agagttccgc
    11041 ctctaagcgt aaatgcttta ccagggggga taatttttcg ggtattatta ataataatgt
    11101 taacgctatt actccccatg tttttatata ttccaggttg caatttgtta ggccaggtac
    11161 cttcaacttc ttcgtttcct ttatttatta taactagtcc atcatgagga gagtgattaa
    11221 tgttcacaga atccccacga ctaaaggcca gaacgttttt agttgcaaat accccctcaa
    11281 atgcgctcca tattcctgct ttatctatat ataatgcgtg gaattcattg tagagctgct
    11341 gataggtaga gcaaccttta ttaagccacg cctcaccgcc gacacgagtg gacccatcat
    11401 catttttaat ctcagtcgtt gattttaata cctgaccatc ccagtaaact aaaggcctgc
    11461 tgaacgggag agcggccata tatgccattg ctaattgttc atcttgctga tggttaaata
    11521 aaaagtcatt gaaaagatta ccattgttag ggaaatcgtg gttgatgtta atcaatatcc
    11581 gtttttctga tgggatgctt gagattgtgg agcccggttt tgatgcaaga gtttggagat
    11641 cgccaccgaa tgaaaaggct tttatcgtct tcatgagcaa tgaaaaatca atggcgctta
    11701 aatcatctct ttcataaagc agcgatgaca tttgttgttc atctaacgga caagttacca
    11761 ggtattccat gtaattcata acagcttttt tttgatattt tggattaaat aaatttgtat
    11821 tgtgcaaccg ctcattataa tttttaagcg gtggggttat cgacctctct atttgttcat
    11881 gttttgagtg ttttgctgca tcgtaacgta aacctcgtac gccatatttt cgcatttcac
    11941 gaacgtattg attttgcaac ataataacct ggtcactttt gggtgttgtt ttaggcattc
    12001 catttaaacg cttttctttt aatgcatgga ggttttccca ttcatcctta gaaatattat
    12061 ctttacttgc ttcgaagtct tttgcagtga gatgtatatc ttctggatgg atttggtcac
    12121 gttcttttgt tactggatct ataataaagg gaatcaagac ctgaccttgc caatagccag
    12181 ggataccact ttctatatct ttactgatct tcgctcgtat atcggcagag ggataatcca
    12241 gatcattttt accactaccc ccgattccca taaagttaaa tgtataatca gggatgatat
    12301 cgataccaaa ttttttggca tctttaacca atgcgataaa atcctgcaaa tctcccattg
    12361 gattatgaat agtacaaaga tcttcgggtt gataagcgtg aaaccagtaa cctttttctt
    12421 cttcgggagt ttctggtccg tcggcgcgga aagtggttac gggagggatt tgtatatacc
    12481 cataatgaca acgtgcaaac tcagatatat ttttcctgat ttgttgatag gtggcattaa
    12541 aagcatgaag aatcgcttcg ccaggttttt tgggggtgat gatttcacat tgtgatttct
    12601 gaacagcagt aagagattta cgctcaagtg tttgccgtag atgttctgga ttgattaata
    12661 ttttaaatgt ttcttgctgg ccggtaggtg acgttacgtt aatattatat acatcagctg
    12721 ccagttcata gtcaggaatg ttaatagcat atgcaacata cttatctttt ggggacggtt
    12781 tgtcagatat aatgtgagta aaatgttcag ctggaccata ctgatgaaca ccgctgttat
    12841 cacctctttc aaccattgta tctcgttgat aaacccatgt ttttccttct cctatcctgt
    12901 ctggcgctgt aatgctacgc tgtgctgctt tattcaactc tcggtaagtt gtatctggaa
    12961 aaagggaaat tttataatcg ttttttaaca gatcattaat aactttttct gcgtgaaaag
    13021 ttgatataat ttctctgtct tttaaatcct gaggataaac tccacaagaa ttaataatat
    13081 ttgttataca gtttagatat tctaatgtat ttaatttgga atttagatcc ccgtcagtaa
    13141 tgttccatga taacaatgtg gggttgccga taggtaaatt tcttggtcct ggttttatag
    13201 aaaacataat aaagttcctc taatggtaga gtaatatggt gtatacggaa acataaaaat
    13261 gtttaattgt tgtgaaagta attgcaaaca gatgtcctac ttttatccaa tatttatatg
    13321 gaaaaggata atgcttaaat ctgttttttt aattatcgat gtggtctgga gaggcaactg
    13381 tcaaaaatcg ctcatacgaa actaatgatt agcagaataa gatctatgaa agaatatatt
    13441 gctatatgaa gtttattttt atgacgtgaa aagtaacaga tatcataatg atgcgaaaac
    13501 tcttcagtta atgtaataaa gttatattat tagatacaaa atcattacaa actatcattg
    13561 aatcgatggt aactataaat agtgctaagt attagcattt gcttgttgac tgagagtatc
    13621 gactttgtga atggaagtgg tgtcgggttt tatgtcgcag gtaatgacga cccaggatga
    13681 atgagaacgc ataaaaatct gttatttaca tcataagatc atcatgttta ttgcgtggta
    13741 tataagtatt gttattgcat actcaaggcc ctttagctca gtggttagag cagacgactc
    13801 ataatcgcct ggtcgctggt tcaagtccag caagggccac caaatcatta ttcacgtatc
    13861 aggaagagtt aaggttacaa gggggactcg atggcggtca atatttcacg actaacgcca
    13921 ttttaaagaa gcagtgcggt aggtagtttc gaatgagggc tggtgttttg gtacgtggca
    13981 cgccactgca atgtatttat ccatcagtac tcattcaaaa gtacctggaa aaaatgcccg
    14041 ctatgccaag cgggcaaatt gcttacatgt ggaattaagt aattaacgat aaagccagag
    14101 tattatcatt tatcatgcct cttcatttaa tcaggttaat tactcaaatc actgaactat
    14161 tctctgttcg ctcactgatg ataagtgaag tgtcacaacc acgatttgta tgtggaccaa
    14221 tgccgtagag taaggatgaa atgtctctgc cacgccttaa acatctacta ctgactagaa
    14281 ccgccgtcga atgcagaaga gtcttgtgag cattacagga gtctggcttt ggtcattaat
    14341 caacgctatt tagatataaa tttctaacac tcaaaaaacg attctcacga ataaattata
    14401 acgggtggtt tttatcatac agtgaaataa ccttttgttt attaaattta acattcctgt
    14461 gctaaattaa ttaaaagtgc catgtatagc atatgaagct gttaatgcca gcaattgtct
    14521 caaatgagcg attttttata gtgacaaaca ataataacat taaaaatagc catgtagaca
    14581 ttaactgagg aatagacaat gttagttagt aaaagcaacg aaattaacac tagcacattt
    14641 ataaatagtg gaagttgtaa tgaaagaaaa tcttctaaat ccatggagtt actagcttat
    14701 agtattataa aattaatttg taaagaagct gcctcaggga cgtataacgg tgctcttgaa
    14761 attttacaaa aaatgatgtc tgaatgtaaa tatcatgaag gcaatgcttt tgtcattatg
    14821 ggagccggag aacaattaaa acgtattaaa tatgatgcta atgaaaataa attaaaagta
    14881 tacaacgtac actttgataa taatcaagag ttagttgctg atggtgagcc tgacgtagta
    14941 tttttaaaaa aaacggtgtg ggaagacctt ctcgttaaac taaagctgga gaacaaagaa
    15001 aatgcggttt ctgaaattaa cctgtcatct aataaaaata atgttgatca gtttattgaa
    15061 tgcgctaaga gaaatgaaca gaccctattc ggcaatataa gaaaaagtga ttttcatgtt
    15121 gcttcacttc agccaggtag aacgcgtagt gttatttcag aaacgccgcc aaatgactgt
    15181 atggaatcac ataatttata tgaaaaccac acagatacgg tttcaactgt aactaaaaat
    15241 tctcagcaag ttaaaggtca ctatggagat aagttgaaag aaatgcagtt gttcctcaac
    15301 cagatgagca atgcacttca acaggattca tctttgttag agtcaaagga acacaccata
    15361 gatattcaag aaaaaacgaa taagtttgtg cagcattttc agcgggtatt atttgataaa
    15421 aatggaaggt catcagagtt tctacttaat ttttatgagt gttgctataa gtttttacca
    15481 agagcgcagc ctcaagataa aatcgatagc tataattcag cactgcaagc tttttccatc
    15541 ttttgttcat ctacgttgac acataataat gtagggttta atttcaaatt atttccagaa
    15601 gtcaagctgt ctggaggaga gcttgaaacg gtattcaaat acaaaaatgg taattttgtc
    15661 tgggagatag ccagaattaa aattactctc ccaaaagaag agggtggttt atataattta
    15721 cgtggattgg attttaaggg atgcttcttt tctggacaga acttcagtaa ctatgatatt
    15781 caatatgtga actggggaac gtcattgttt gatcttgata ctccatgtat ttttaatgcg
    15841 cctgcttaca acaagagtaa tgaaaaatca ttagaacgcg tcagcgaaaa cggtttaagt
    15901 ggagtcttgt ctgatcgtaa taaaaaaata aaactaatca cgggtgtggc accattcgat
    15961 gatattttat ttatggatga tgactttgat gataactccc ctgatgatgg tcccgttgag
    16021 aatagtcctg ttgtgaatag tccccttgta tgaagttcta aaagtagttt tcaatgataa
    16081 ataaatggct atttttacga agagataata acgtagattg aaacaatatg tattattgat
    16141 taagtaaatg gggttattct tcacactaca tccattttag tgtcactacc aaagaaaaag
    16201 gggctacgtt ttcatgtaac cccttgattt atttggtggg ctagcagtga ctgaattggt
    16261 gctgtaattg gttgaaatat ttcatctaat gtctggttca actttcctta gctgcctgaa
    16321 agtttttttg atattaccca gcaataacag cgttacggtg taccacgcct gactgatgaa
    16381 ctgcgtgctc agagttacca cttcagcgca taaccgtggc agcagacccg gactgaaaaa
    16441 ctgctcagga cattatcggc agggtacgca ctgctagtct gtaaactgaa ccgactgggg
    16501 cgcagtatgt ggcatcttgt cgtgctgttg gaggagctgt gcaaacgtgg tattaacttt
    16561 cgtgctctgg cccaatctat atttccccaa caatgggggg acgaatgctg taaaagtaaa
    16621 acaatctgcg atctcaaagt tattgtgtga tttttatgtg agcagaagat attcatcaga
    16681 aacgattatg taaatcattt tatttttccg acggcttcaa tgtcgaaaga aaagaatacg
    16741 ccgtatttcc tgaaaagatt gactttttta tccaatcatc cttcagcaca atgtcttaaa
    16801 aaaatgcctc attcttatgc ggaatatcat catttcatca tgatgtcttt gatgagcggt
    16861 gaacacaata cacttgcgct gtctcttcag gatgaatccc ctggtctggt gcctgtgggc
    16921 tgatgttgca gcaaagctaa ggtcgcttaa acgctactca gtattcactt ttcagaggat
    16981 gaaatttatg aacaggacca gtccctatta ttttcgccgc tcagtacttt ccttattgat
    17041 atctgcctta atatatgccc agcccgggat ggcggccttc actactaatg ttattggtgt
    17101 ggtaaacgat gagactgtag atggcaacca acgagtagat gaacgaggta caacaaataa
    17161 cactcatatt atcaaccatg gccggcagga ggtctatggt gggatatcta atagtagtat
    17221 tattgaaact ggtggcgagc aactagtatc aatccatgcc gatattaacg ggcaagctaa
    17281 taatacaacc attaatggag gcagacagtc aattgagtac ggaggaattt ctactggcac
    17341 gataatcgaa agtggcaatc aatatgttta taaaggtggt accagcaatg acacgacaat
    17401 taagggcggt acttcacgca tagagggggg gactgcaaat ggaacaatca ttgatggtgg
    17461 aggccagtca gtatcaactc aggggcatgt tgacggtacg acgataaata agtctggtta
    17521 tcaggacatc acgcaaggaa gtctggcgac gaacacaacc ataaatggtg ggcgacagta
    17581 tgttgaacag agcacagtag aaacaaccgc cattaaaaat ggcggtgagc aaagagtata
    17641 tgagagccgt gcgctggaca cgacgattga aggcggaact cagtctctga atagtaagtc
    17701 aacggcaaaa aatacgcaga tctattctgg tggtacgcaa attgttgata acacgagctc
    17761 ctcggatgtt attgaagttt attccggcgg cgtgcttgat gttagaggtg gtacggcaac
    17821 taatattact cagcacgatg gagccgcttt aaaagtaacg acttacgatt tgacggtgag
    17881 cggtacgaat agtgaagggg cattctccat ccacaataac gtggcagaga atgtgttgct
    17941 ggaaaacggt ggtcatttag acataaacgc atatggttcg gcaaataaga cgattattaa
    18001 agataaagga acaatgtcag ttttaacgaa tgctaaagct gatgcgaccc gaatagataa
    18061 tggcggcgtt atggatgttg ccggaaacgc gacaaatacc ataattaatg gtggcacaca
    18121 gaatattaat aatcatggta ttgccacggg caccaatatc aacagtggaa cgcaaaatat
    18181 caagagcggc gggaaagctg acacgacaaa tatatcctct gggagccggc aggttgttga
    18241 gaaagatggt acggcaactg gcagcaatat tagcgccgga ggctcgctga ttgtctatac
    18301 cggcggtatt gcacatgggg ttaaccagga gacgggcagt gctttagttg ccaacacggg
    18361 cgcagggact gatatcgaag gatacaacaa gctctctcac ttcactatta ccggaggaga
    18421 ggctaattat gttgtgctgg aaaataccgg cgaactgacg gtaatggcta aaacctcggc
    18481 gaaaaatact accattgatg ctggcggtaa gctgattgtc cagaaggagg ctaaaacaga
    18541 tagcaccaga cttaataatg gtggcgttct ggaggttcag gacggtggtg aggctaagca
    18601 tgttgagcaa caatccggcg gcgcattaat tgcttccacg acctccggaa cacttatcga
    18661 aggaaccaac agttatggtg atgctttcta catcaggaat tcagaagcta aaaatgtagt
    18721 gctggaaaac gctggctcat taacagtcgt cactggttcc cgggcagttg acacgattat
    18781 taatgccaac ggcaaaatgg atgtttatgg aaaagatgtt ggtactgtac ttaatagtgc
    18841 tggtacccaa acaatatatg tcagtgccac ttctgaaaaa gcaaatatca aaggtggcaa
    18901 gcaaacggta tatggtttag ccactgaggc aaatatcgaa agtggtgaac aaattgttga
    18961 tggtgggtca acagacaaaa cgcacatcaa tggcggtacg caaaccgttc agaattatgg
    19021 taaggcgatc aataccgata tcgtctctgg cctacaacaa attatggtaa acgggacagc
    19081 ggaaggttcc attattaatg gcggttcaca ggtagttaat gagggcggtc tggctgaaaa
    19141 ctcggtgctt aatgaaggcg gcactctcga tgtgcgggag aaaggcagcg caacggggat
    19201 acagcaaagt agccagggcg cgttggttgc aaccaccaga gcgacgcggg tcacagggac
    19261 acgcgctgat ggcgtcgcgt tcagcattga gcaggatgcg gcgaacaata tcctgctggc
    19321 agatggcggc gtgttaaccg tggagtcaga caccacttcc gccaaaacac aggtcaatgc
    19381 gggcgggcgg gagatcgtca aaacaaaagc cactgcgaca ggcacgacgc tcaccggcgg
    19441 cgaacaaatc gtcgaggggg tggcgaatga gacaacgatt aacgacggcg gaatacaaac
    19501 agtttcggct aacggtgagg cagtaaaaac aacgattaat gaaggcggta cgctgacagt
    19561 caacgataat ggcaaagcga cagatatcat ccagaacagc ggtgccgctc tccagacgag
    19621 cacggctaac ggtattgaaa tcagcggtac tcaccagtac ggcacctttt ccattgccgg
    19681 aaatttagcg accaatgtgc tgctggaaaa tggcggtaat ttattggtat tagcaggtac
    19741 cgaagctcgc gattccacgg ttggcaacgg tggggcaatg caaaacctgg gtcaggactt
    19801 cgccacaaag gttaattccg gtgggcaata tacccttggt cggtcaaaag atgagtttca
    19861 ggctctggcc cgggcagaag atctactgat cgctggcggg acggcaatcg tctacgcagg
    19921 tacgctggcg gatgcatcgg tcagtggcgc gaca
//
LOCUS       DAPOZO010000088        19745 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPOZO010000088 DAPOZO010000000
VERSION     DAPOZO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.088"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(6..212)
                     /locus_tag="ROI21_003515"
     CDS             complement(6..212)
                     /locus_tag="ROI21_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDX5112396.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSEKPVWMDQAEKATGDFY"
     gene            524..613
                     /gene="kdpF"
                     /locus_tag="ROI21_003516"
     CDS             524..613
                     /gene="kdpF"
                     /locus_tag="ROI21_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDX5112397.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            613..2286
                     /gene="kdpA"
                     /locus_tag="ROI21_003517"
     CDS             613..2286
                     /gene="kdpA"
                     /locus_tag="ROI21_003517"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDX5112398.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     VLFRLPGVSDSEMNWKQYLSAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2309..4357
                     /gene="kdpB"
                     /locus_tag="ROI21_003518"
     CDS             2309..4357
                     /gene="kdpB"
                     /locus_tag="ROI21_003518"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDX5112399.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWITVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLCEPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASELIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARLGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4366..4938
                     /gene="kdpC"
                     /locus_tag="ROI21_003519"
     CDS             4366..4938
                     /gene="kdpC"
                     /locus_tag="ROI21_003519"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDX5112400.1"
                     /translation="MRGLRPTLSTFLFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASANVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4931..7615
                     /gene="kdpD"
                     /locus_tag="ROI21_003520"
     CDS             4931..7615
                     /gene="kdpD"
                     /locus_tag="ROI21_003520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDX5112401.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPTLHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSTINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7612..8289
                     /gene="kdpE"
                     /locus_tag="ROI21_003521"
     CDS             7612..8289
                     /gene="kdpE"
                     /locus_tag="ROI21_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDX5112402.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8564..8668
                     /locus_tag="ROI21_003522"
     CDS             8564..8668
                     /locus_tag="ROI21_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDX5112403.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            8978..11176
                     /gene="speF"
                     /locus_tag="ROI21_003523"
     CDS             8978..11176
                     /gene="speF"
                     /locus_tag="ROI21_003523"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HDX5112404.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATANEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYETFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11173..12492
                     /gene="potE"
                     /locus_tag="ROI21_003524"
     CDS             11173..12492
                     /gene="potE"
                     /locus_tag="ROI21_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDX5112405.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCVIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12549..14189)
                     /gene="pgm"
                     /locus_tag="ROI21_003525"
     CDS             complement(12549..14189)
                     /gene="pgm"
                     /locus_tag="ROI21_003525"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDX5112406.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14215..14760)
                     /gene="seqA"
                     /locus_tag="ROI21_003526"
     CDS             complement(14215..14760)
                     /gene="seqA"
                     /locus_tag="ROI21_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDX5112407.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            14945..15709
                     /gene="ybfF"
                     /locus_tag="ROI21_003527"
     CDS             14945..15709
                     /gene="ybfF"
                     /locus_tag="ROI21_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDX5112408.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15849..16142
                     /gene="ybfE"
                     /locus_tag="ROI21_003528"
     CDS             15849..16142
                     /gene="ybfE"
                     /locus_tag="ROI21_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDX5112409.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16282..16812
                     /gene="fldA"
                     /locus_tag="ROI21_003529"
     CDS             16282..16812
                     /gene="fldA"
                     /locus_tag="ROI21_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDX5112410.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17022..17108
                     /gene="uof"
                     /locus_tag="ROI21_003530"
     CDS             17022..17108
                     /gene="uof"
                     /locus_tag="ROI21_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDX5112411.1"
                     /translation="MIRIISRANSVTSPNEVNRLVTGQIPHD"
     gene            17101..17547
                     /gene="fur"
                     /locus_tag="ROI21_003531"
     CDS             17101..17547
                     /gene="fur"
                     /locus_tag="ROI21_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDX5112412.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17631..17957)
                     /locus_tag="ROI21_003532"
     CDS             complement(17631..17957)
                     /locus_tag="ROI21_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112413.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18007..19413)
                     /gene="chiP"
                     /locus_tag="ROI21_003533"
     CDS             complement(18007..19413)
                     /gene="chiP"
                     /locus_tag="ROI21_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDX5112414.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4538 a         4979 c         5252 g         4976 t
ORIGIN      
        1 agcaatcagt aaaaatcacc agttgccttt tctgcctgat ccatccacac cggtttctcg
       61 ctggttttcg accagacgcg gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa
      121 agcatgaaag gcagcgccag aacgcccgct gcaaccgcat aagtacggcg taaaaagata
      181 agccatgcag gatattctct gtagagttcc atacttttct cccccttttg tatctacccg
      241 gtgaatggca ccggaaaaat gaatttgttt atctgatgaa aatagtaccg ccttttgtgt
      301 aattttacta ctcatccgac cacttatttt tgcttattga tggtttattt acattcatcg
      361 tgtaattaag ttacacaaaa gttaaattaa tactaaccat cagttaaatc atggcttttg
      421 ccattttata ctttttttac atcccgcccg aagatttttg cgaaatcttt gcagccagaa
      481 ttctaccctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg caggcgtgat
      541 agccggcgtg ttgctggtgt ttttattact gggttatctg gtttatgccc tgatcaatgc
      601 ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt actggtgtta
      661 atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaacga catacctctt
      721 cccggtacag cgggtgttga acgcgtactt tttcgtttac ctggcgtctc tgacagtgag
      781 atgaactgga agcagtatct ctctgccatt ctcggcctga acatgctggg gctggcggtg
      841 ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca gttgccaggg
      901 ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcaccaatac caactggcaa
      961 tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcgggctt aacggtgcaa
     1021 aactttcttt ctgccgccag cgggattgcg gtgatttttg ccctcatccg tgcatttacc
     1081 cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat cacgttatgg
     1141 gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg tgcactgcaa
     1201 aactttctgc cctatcaggc ggtgaatacc gttgaaggag cgcaacagct gttacccatg
     1261 ggtccggtgg cttctcagga agcgatcaag atgctcggta ctaacggcgg tggcttcttt
     1321 aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt cgtgcagatg
     1381 ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt gacgggcgat
     1441 cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat ctgcgtaggc
     1501 gtggtgatgt gggcagaagt tcagggtaat cctcatctgc tggcgctggg tgcggacagc
     1561 agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag cctgtttgcg
     1621 gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc gtttaccgct
     1681 ctcggtggca tggtgccgat gtggctgatg caaattggtg aagtggtatt cggcggtgtc
     1741 ggttctggtc tttacggcat gatgctgttt gttctgctgg cggtgtttat tgccgggctg
     1801 atgattggtc gtacaccgga atatctgggt aaaaaaatcg acgtacgcga gatgaaactg
     1861 actgcactgg cgattctggt tactccaaca ctggtgctga tgggcgcggc gctggcaatg
     1921 atgaccgacg ccggacgtag cgccatgctc aaccctggcc cgcatggttt tagcgaagtg
     1981 ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg attaagcgcc
     2041 aactccccat tctggaactg tttactggcg ttctgcatgt ttgtcggtcg cttcggggtg
     2101 attatcccgg tgatggcaat tgccggttcg ttggtgagta aaaagagcca acccgccagc
     2161 tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg caccgtgttg
     2221 ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc ggaatatctc
     2281 tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc tattcgaacc
     2341 aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc aggcgcaatg
     2401 gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct gtattagcat
     2461 cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcca ttagcggctg
     2521 gctgtggatc accgtactgt tcgctaattt cgccgaggcg ctggcagaag gccgcagtaa
     2581 agcgcaggcc aacagcctga aaggggtgaa aaaaactgcc tttgcccgca agctgtgtga
     2641 gccgaaatat ggcgctgcgg cggacaaagt tcccgccgat caactgcgta aaggcgatat
     2701 cgtactggta gaagctggcg atattatccc ctgcgatggt gaagtcattg aagggggcgc
     2761 atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg aatccggcgg
     2821 cgattttgcc tccgtcactg gcggcacgcg tattctttct gactggctgg tgattgagtg
     2881 cagcgttaac cccggcgaga catttctgga taggatgatc gcgatggtgg aaggcgcaca
     2941 gcgacgcaaa acgccgaacg agattgccct gaccattctc ctgattgccc tgactatcgt
     3001 ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta atgcagtgag
     3061 tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg gcggcctgtt
     3121 gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga ttgccaccag
     3181 cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata aaaccggcac
     3241 catcacactc ggtaaccgtc aggcgtcgga gcttatcccc gcgcagggcg tggatgaaaa
     3301 aacgctggct gacgccgcac aactggcttc actggctgat gaaacgccgg aaggccgcag
     3361 tattgtgatc ctcgccaagc agcgttttaa cctgcgcgag cgcgatgtgc agtcgctcca
     3421 tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca tcgacaaccg
     3481 catgatccgt aaaggttctg tcgatgccat tcgccgccat gttgaggcta atggtggtca
     3541 cttccctgcc gacgtcgatc aaaaagtcga tcaagtagca cgcctggggg caacaccgct
     3601 ggtcgtggtg gaaggttccc gcgtgttggg cgttattgcg ctaaaagata tcgtcaaagg
     3661 cggtattaaa gagcgcttcg cccaactgcg caaaatgggc attaaaacgg tgatgattac
     3721 cggcgataac cgtctgactg ccgccgccat tgctgcggaa gcgggcgtcg atgatttcct
     3781 cgccgaagcg acaccagaag ccaagctggc attgattcgt cagtatcagg cagaaggccg
     3841 tttagtggcg atgaccggcg atggcaccaa cgatgctccg gcgctggcgc aggcagatgt
     3901 cgcggtggcg atgaactccg gtactcaggc ggcgaaagaa gcgggcaata tggtggatct
     3961 cgactctaac ccgaccaagt tgatcgaggt ggtacatatt ggcaaacaga tgctgatgac
     4021 ccgtggctcg ctgaccacct tcagcattgc caacgatgtg gcgaaatact tcgccattat
     4081 tccggcggcg ttcgcggcaa cgtatccaca gttaaatgcg ctgaacatca tgcgcctgca
     4141 ttcgcccgac tccgcaatcc tcagtgcggt gattttcaac gccttgatta tcgtcttttt
     4201 gattccccta gcgttaaaag gcgtgagtta taaaccgctt accgcttctg ccatgttgcg
     4261 ccgtaactta tggatttacg gtctgggtgg gctgctggtg ccgtttatcg gtatcaaagt
     4321 cattgattta ctgctgaccg tttgcggtct ggtgtgaggt ttaccatgcg tggattacgt
     4381 ccgacattat cgacatttct ctttctgtta ttgattactg gcggcgttta cccgctgcta
     4441 accaccgtgc tggggcaatg gtggtttccc tggcaggcca acggttcgtt gattcgtgaa
     4501 ggtgatacgg tgcgcggttc ggcattaatc gggcagaatt ttaccggcaa cggctatttt
     4561 cagggtcgcc cgtcggcaac ggcagaaatg ccctataatc cacaggcttc tggcgggagc
     4621 aacctggcgg tcagtaaccc tgagctggat aaactaatag ccgcacgcgt tgctgcatta
     4681 cgggccgcta acccggatgc cagcgcgaac gttccggttg aactggtgac ggcatcggca
     4741 agcgggctgg acaataacat caccccgcaa gcggcagcct ggcagatccc acgcgtggcg
     4801 aaagcgcgca atctcagcgt tgaacagctc acgcagctga tcgcaaaata cagccaacaa
     4861 ccgctggtga aatatatcgg ccagccggtc gtcaacattg tcgaactcaa tctggcgctg
     4921 gataaacttg atgaataacg aacctttacg tcccgacccc gatcgtctgc tggaacaaac
     4981 tgccgcgccg catcggggga agctgaaagt tttcttcggt gcctgcgcag gcgtcggaaa
     5041 gacctgggcg atgctggcag aggcccagcg actgcgggcg caagggctgg atattgtggt
     5101 tggcgtggtt gaaacccacg ggcggaaaga taccgccgca atgctggaag ggctggctgt
     5161 tctgccgccc aaacgccagg cgtaccgtgg gcggcatatc agcgagtttg atctcgatgc
     5221 cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa ctggcgcaca gtaatgcgcc
     5281 aggttctcgt catcctaaac gctggcagga catcgaagaa ctgctggaag cgggcattga
     5341 tgttttcact accgtcaacg ttcagcatct ggaaagcctg aatgatgtgg tcagtggcgt
     5401 caccggaatt caggtacgag aaaccgtgcc cgatcctttt ttcgatgccg ccgacgacgt
     5461 ggtgctggtg gacttgcccc cggacgatct gcgtcaacgg ctgaaagaag gtaaagtcta
     5521 tattgccggg caggcggaac gcgccattga acattttttt cgtaaaggta atctgatcgc
     5581 cctgcgcgaa ttggcactgc gccgtactgc cgatcgcgta gatgagcaaa tgcgcgcctg
     5641 gcgggggcat cctggcgaag agaaagtctg gcacacgcgc gacgcgatcc ttttatgcat
     5701 cggccataac actggtagtg agaaactggt ccgcgcggcg gcgcggctgg cgtcacggct
     5761 gggtagcgtc tggcacgcgg tgtatgttga aacccctacc ctgcatcgct taccggagaa
     5821 aaaacgtcgg gcaattctca gcgccttacg tctggcgcag gagctgggcg cagagacggc
     5881 aacactttct gatccagcgg aagagaaagc ggtagtgcgt tatgcccgtg aacataatct
     5941 cggcaagatt attctcggtc gcccagcctc gcgccgctgg tggcgtcggg aaacgtttgc
     6001 tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg ctggtcgcgc ttgatgaacc
     6061 acccgcccgc acgattaaca acgcgccgga tagccgctct tttaaagaca agtggcgggt
     6121 acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc gttatcacct taattgccat
     6181 gcagtggctg atggcgtttg atgccgccaa cctggtgatg ctgtatctgc ttggcgtggt
     6241 ggtggtggca ctgttttatg ggcgctggcc ttcggtggtc gccaccgtca ttaatgtggt
     6301 gagtttcgat ctctttttta tcgccccacg cggcacgctt gccgtctctg atgtgcaata
     6361 tctgctgacc ttcgcggtga tgttaaccgt cgggctggtg atcgggaacc ttactgcggg
     6421 cgtgcgttat caggcgcggg tagcccgtta ccgcgagcaa cgcacacggc acttatatga
     6481 aatgtcgaaa gctctggcgg tgggccgcag tccgcaggac atcgccgcca ccagcgaaca
     6541 gtttattgcc tccacgtttc atgcccgcag tcaggtgctg ttacccgatg acaacggtaa
     6601 attgcagccg ttaacacatc cgcaaggaat gaccccgtgg gacgatgcca tcgcgcagtg
     6661 gagttttgat aaaggtctgc ctgcgggcgc gggcaccgac acgttacccg gtgtaccgta
     6721 ccagattttg ccgctaaaaa gcggcgagaa aacctacgga ctggtggtgg tggaaccggg
     6781 gaatctgcgc cagttgatga tcccggaaca gcagcgcctg ctggagacat ttacgttgtt
     6841 agtcgccaat gcccttgagc ggctgacact gaccgccagc gaagaacagg cgcggatggc
     6901 aagcgaacgt gaacagatcc gcaacgccct gctggcggcg ctttcgcatg atttacgcac
     6961 tccgctcacg gtgctgttcg gtcaggcaga aatcttaacg ctcgatctgg cgagcgaagg
     7021 atcgccccac gcccgccagg ccagcgagat tcgtcagcat gtgctgaaca ccacccgact
     7081 ggtgaataat ctactggata tggcacgaat tcagtccggc ggctttaatt tgaagaaaga
     7141 gtggttaacg ctggaagaag tggtcggcag cgcgctgcaa atgctggaac cgggtttatc
     7201 gtcgaccatc aatctttctc tgccagaacc gctgacctta atccacgttg acgggccact
     7261 ctttgaacgg gtgctgatta atctgctgga gaacgcggtg aaatacgcgg gggcgcaggc
     7321 cgaaattggt atcaatgccc acgttgaggg cgaaaatcta caactggatg tctgggataa
     7381 cggccccggt cttccaccag gccaggagca gacgatattt gataagtttg ctcgcgggaa
     7441 taaagagtcg gcggtaccgg gtgtagggct tgggttggca atttgtcggg cgatagtgga
     7501 tgtacacggc ggcaccatta ccgcattcaa ccgaccggaa ggtggtgcct gttttcgtgt
     7561 tacacttccc cagcaaacag cccctgaact tgaagaattt catgaggata tgtgacaaac
     7621 gttctgattg ttgaagatga acaggctatt cgtcgctttc tgcgcacggc gctggagggc
     7681 gacgggatgc gcgtctatga ggccgaaacg ctgcaacgcg gcttgctgga agcggctacc
     7741 cgtaagccag atttgattat tctcgatctc ggcctgcccg atggtgatgg gattgagttt
     7801 atccgcgacc tgcgccagtg gagcgcagtg ccagtgattg tgctttccgc acgcagcgaa
     7861 gagagcgaca aaatcgccgc gctggatgcc ggagcggatg attacctgag taagccgttt
     7921 ggcattggcg aattgcaggc ccgtctgcgc gtcgctttac gtcgccactc cgccaccgct
     7981 gcgcccgatc cgctggtgaa attttccgat gttaccgtcg atttagccgc ccgcgtgatc
     8041 catcgcggtg aggaagaggt gcatctcaca ccaattgagt ttcgcctgct ggcggtgctg
     8101 ctcaacaatg ccggaaaagt gctcacccag cgccagctcc ttaaccaggt gtgggggcca
     8161 aacgcggtcg aacacagtca ttatttgcgt atttatatgg gacatctgcg acaaaaactg
     8221 gaacaggatc ccgctcgccc ccgccatttc attactgaaa ccggtattgg gtatcggttt
     8281 atgctttgaa ttgttattca ataaattcgc tttaatagtg attaatttat tattaaacct
     8341 gctccaaata ttattatttg gcaggtttaa tttcttaacg caaatataaa acacaaaatt
     8401 acaacattta agtaacaaaa cagcatctaa tatggcaata ttcataatta atgatttatt
     8461 atctggggta tgatcgtttt ttgttgatct tcttcacaga ttatagccat ttcatggata
     8521 gaataactct accttcaact gacacagcaa gaggtaaagg taaatggaaa ataataaccg
     8581 cttaatgcct catataaggc ggacaaccca tatcatgaag tttgcccatc gtaatagttt
     8641 tgactttcat ttctttaatg cccgctagtc ttctgactaa agggcacccc aacgtacagg
     8701 tctccctgac tttaagcatt acaggttaat acctgtattc ctcggtgctc atatactgct
     8761 aaccctttta aactctaaat aattcgagtc gcagcacttg caacttgagg tatgacgagt
     8821 atagccagtt accgggctgg tctgggttat tgcatctaca aaaagcaaac tactgattta
     8881 tttatcagcg gtggggtttt gcttttttcc ggcgtgatca atttctcctt tgagaaattg
     8941 aggacctgct attacctgaa ataaagagat gaacaaaatg tcagaattaa aaattgccgt
     9001 tagccgttct tgcccggatt gtttttccac tcatcgtgca tgcgtgaata ttgacgaaag
     9061 taattatatt gacgttgccg ccattatttt atcagtcaat gatgttgaac gtggaaaact
     9121 cgatgaaata gacgctactg gctatggcat tcctgttttt attgcaacgg caaatgaaga
     9181 acgtgtacca gcagaatatc tgccacgtat ttctggtgtc ttcgagcatt gcgaatcacg
     9241 gaaagagttt tatggacgtc agttagaaac cgctgctagc cattatgaaa cgcaactgcg
     9301 cccgcccttc ttccgcgcac tggtcgatta tgtcaatcag ggcaatagcg cttttgactg
     9361 cccgggtcat cagggaggtg agtttttccg tcgtcatccg gcagggaatc agttcgtgga
     9421 gtacttcgga gaaacgctgt tccgttccga cttatgcaac gcggatgtgg caatgggcga
     9481 tctgctgatt cacgaaggcg cgccatgcat tgcacagcaa catgcggcaa aagtgtttaa
     9541 tgccgataaa acatacttcg ttctaaatgg cacttcatct tctaacaaag tggttttaaa
     9601 cgccctgcta acaccgggtg atctggtgct gtttgatcgc aataaccaca aatctaacca
     9661 ccacggagcg ttgctacagg ctggtgcaac accggtttat ctggaaacgg cacgtaaccc
     9721 gtatggcttt atcggtggca ttgatgcgca ttgttttgaa gaaagttacc tgcgtgagct
     9781 gatcgcggaa gtcgcaccgc agcgggcaaa agaggctcgt cctttccgcc tcgctgtgat
     9841 tcagttaggc acctacgacg gtacgattta taacgcccgc caggtggtgg ataaaattgg
     9901 tcatctgtgt gactacattc tgtttgactc cgcatgggtc ggctatgagc aatttatccc
     9961 gatgatggct gactgttcgc cgctgttgct ggatcttaat gagaacgatc cgggtattct
    10021 ggttacgcaa tccgtacata aacagcaggc tggtttttct caaacttcac aaatccataa
    10081 aaaagacagc cacatcaaag ggcaacagcg ttatgtaccg cacaaacgca tgaacaacgc
    10141 ctttatgatg cacgcctcca ccagcccgtt ctatccgctg tttgccgcac tggatatcaa
    10201 cgccaaaatg catgaaggtg tcagcggtcg taatatgtgg atggattgtg tggtaaatgg
    10261 cattaatgcc cgcaaactga tcctcgataa ctgtcagcat attcgtccgt tcgtacctga
    10321 actggtggat ggtaaaccct ggcagtcgta tgaaacagcg caaattgcgg ttgatctgcg
    10381 cttcttccag tttgtaccag gggaacactg gcattctttt gaaggctatg cagagaatca
    10441 atactttgtc gatccttgca aactgttgct gacaacccca ggtattgatg cacgtaacgg
    10501 cgaatatgaa acattcggtg tacccgcgac gattcttgct aacttcctgc gcgaaaatgg
    10561 cgtagtgccg gaaaaatgcg atcttaactc catcctcttc ctgctgactc cggcggagga
    10621 tatggccaaa cttcagcaac ttgttgccct gctggtacgc ttcgaaaaac tgctggaatc
    10681 tgatgcgccg ctggcggaag tgctaccttc catctacaaa cagcatgaag agcgctacgc
    10741 cggttatacc cttcgtcagt tgtgccagga aatgcatgat ttgtatgccc gccacaacgt
    10801 gaaacaactg caaaaagaga tgttccgtaa ggaacacttc ccacgcgtca gcatgaatcc
    10861 gcaagaagct aactacgcct atttacgcgg tgaagtggag ctggttcgtc tgccggatgc
    10921 agaaggccgt atcgctgccg aaggtgcgct tccttatcct ccgggtgtgc tgtgtgttgt
    10981 tccgggtgaa atctggggtg gtgctgtttt gcgttacttc agcgctctgg aagaagggat
    11041 caacctgctg ccaggttttg caccggagct gcagggtgtc tatatcgaag aacatgatgg
    11101 tcgtaagcaa gtttggtgct atgtcatcaa gcctcgtgat gcgcaaagca ccctgttgaa
    11161 aggggaaaaa ttatgagtca ggctaaatcg aacaaaatgg gcgtcgttca gttaaccata
    11221 ctgacgatgg tcaacatgat gggctccggt atcatcatgc tgccgacaaa gcttgccgaa
    11281 gtcgggacaa tctcaattat ttcctggctg gtgacagccg taggctcaat ggcactggca
    11341 tgggcattcg caaaatgcgg tatgttcagc cgtaaatcag gcggtatggg cggttatgcg
    11401 gagtatgctt tcggtaaatc cgggaacttt atggcgaact atacctacgg cgtctcactg
    11461 ctaattgcta acgtcgcgat tgctatttcg gcggttggtt acggcaccga actgctcggc
    11521 gcgagtttgt cgccagtgca gattggtctt gcgaccatcg gggtgctgtg gatttgtacc
    11581 gtggctaact ttggtggtgc gcgcattacc gggcaaatca gtagcattac cgtgtggggg
    11641 gtcattattc cggtggtagg cctgtgcgtt atcggctggt tctggtttag cccgacgctt
    11701 tacgttgatt cctggaatcc gcatcatgca ccgttcttca gtgcggtagg ttcttccatc
    11761 gctatgacgc tgtgggcttt tcttggtctg gagtctgcgt gtgcgaatac tgatgtagtg
    11821 gaaaacccgg aacgtaatgt gccaatcgcg gtactcggcg gtacgttagg tgcggcggtg
    11881 atttatatcg tctccaccaa cgtgattgca gggattgtgc ctaatatgga gctggcaaat
    11941 tcaacggcac catttggcct ggccttcgca cagatgttca cgccagaagt gggtaaagtc
    12001 attatggcgc tgatggtgat gtcctgctgc ggttcgctgc ttggctggca gttcaccatt
    12061 gcccaggtgt ttaaatcttc atctgatgaa ggctacttcc ctaaaatttt ctcccgtgta
    12121 accaaagtgg atgcaccggt gcagggaatg ttgaccattg tgattatcca gagcgggttg
    12181 gcgctgatga cgattagccc gtcgctaaac agtcagttca acgtgctggt taacctggcc
    12241 gtggtgacca atatcattcc gtatattctg tcaatggcgg cattagtcat tattcagaag
    12301 gtcgctaatg tgcccccctc aaaagcgaaa gttgcaaact ttgttgcttt tgttggcgcg
    12361 atgtacagct tttatgcgct gtactcatcc ggggaagaag ccatgctata cggttccatc
    12421 gtgaccttcc tcggttggac actgtatggt ctggtctcac cacgctttga actgaaaaat
    12481 aaacacggtt aattaagaaa agcaaaaaag ggcgatcttc cgaccgccct ttttcttatt
    12541 taatgtgttt acgcgttttt cagaacttcg ctaacaatct caaccgcttc tttctcgatc
    12601 tgcttgcgat gctcttcacc gaggaagctt tcgcaataaa tcttgtacgc gtcttccgtg
    12661 cctgacggac gcgcggcgaa ccagccgttg tcagtcatca ctttcagacc gccgatagaa
    12721 gcaccattgc ccggtgccgc agtcaggcgc gcggtgatcg ggtcacctgc cagggtgctg
    12781 gcgctcacca tttccggaga cagcttagac aacgccgctt tttgtgcgga agtcgcagct
    12841 gcctgcaaac ggttgtagct cggcgcacca aagcgttttg ccagttcgtt gtagtgttcc
    12901 tgcgggttct taccggtgac agcggtgatt tccgccgcca gcagacacat gatgatgccg
    12961 tctttgtcgg tggaccacgg cgtgccgtcg aaacgcagga aggaagcccc tgcgctctct
    13021 tcgccgccaa agccgaagct gccgtcgaac agaccatcaa caaaccattt gaaacctacc
    13081 gggacttcta ccagcttacg gcccaagtca ttgaccacac ggtcgatcat cgcagatgaa
    13141 accagcgttt taccgacagc aacatctttg ccccactgcg gacgatgctg gaacaggtag
    13201 ttgatcgcca ccgccaggta gtggttcgga ttcatcaaac ctgccggagt gacgataccg
    13261 tgacggtcat aatccgggtc gttagcaaac gccagatcga acttatcacg cagtgccagc
    13321 aggcccgcca tcgcacactc ggaggagcag tccatacgga tcgcaccgtc tttatcgagg
    13381 tgcataaagc ggaaggtttg atcgacctga tcgttaacga tggtcaggtt gaggttgtaa
    13441 tactcgccaa tgcgcttcca gtattcgata ccggaaccgc ccaacggatc aacgcccagc
    13501 gtcaggcccg ctttctgaat cgccgccata tcaacgatat cggccagccc ttccacgaac
    13561 ggctgcacca gatcctgctc tttcacatga ccggatgcca tcgcttcgtc gagggagata
    13621 cgcttcacgc ctttcaggcc atcggccatc agtgcgttgg ccctgtcttc caccactttg
    13681 gtgacgttgg tatcagccgg gccaccattt ggcggattgt acttgatacc accatcttcc
    13741 ggcgggttat gggacggtgt aatcacgata ccgtctgcca gcgggccacc ttttttattg
    13801 tgaaccagga tggcattgga aacggcaggc gtcggagtga agccattgtt ttcctgcaca
    13861 ataacatcta cgccgttcgc tgccagcact tccagtacgg aaatgaacgc aggctcggac
    13921 agagcgtgag tatctttacc cacatagcaa gggccagtga tgccgttttt cgcacgttct
    13981 tcagcaattg cctgagcgat cgccagaatg tgcggctcgt taaagctgtg gcgcgctgca
    14041 ctgccacggt gaccagaagt accgaatttc accgcgtgct ccgcattccc tgcttctggt
    14101 ttcagtacat aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact ctgttgtgca
    14161 ggttggcctg cacgattgtg gattgccatt gctttgtcct ttgtctgcaa cgttttagat
    14221 agttccgcaa accttctcaa tcaattccgc cgggaattgc atcgactgca tgatgtgttc
    14281 gatcatgctg catttacggc cggtgttggt gttggtgatc acccaatacg gcgtgcctgg
    14341 cacatgtttc ggcttggtct gattaccatt tttcagcagc gtttgttcat ctgccgcaaa
    14401 gtaaacgcgt gtacgaccgt gcaacgattc cgttgcttcg gcaaacgcct gggcgtcaag
    14461 agaatatagt gtagacaaca gcagcataaa gcgattgacc gctcgctttt gctctgcgta
    14521 ttcatccgaa agcagaagtt cacgcattgc gcgaaccttg tctttaatcg ttttgaccgg
    14581 cttcgcttcg acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg gagcagcagg
    14641 ctgtgatgcg gcggaaaatt tcaacatacg ccgtaaaatg tcggatgcgc tctcgccgat
    14701 atgcttagtg tggctggcaa tatagctgta gagttcatca tcaacttcaa tcgttttcat
    14761 cttaatccag tgcagtgtct tagctgaata caagtcgcca ggagtatatg aataaatccc
    14821 ggcagcggat agcgtcaagc ctggcttgca gaaaaactcg gaataaacct tatttgttgc
    14881 agccctgtgg cacaatggtc aacataatac cctaacccga caacgcgaaa aaaagaactt
    14941 tgccatgaaa ttgaatatcc gcgcgcaaac tgcacaaaac cagcacaata attctcccat
    15001 cgttcttgtc catggtctgt ttggtagcct agacaacctt ggcgtactgg ctcgcgatct
    15061 ggtaaacgat cacaatatca tccaggttga tatgcgtaac cacggtcttt caccgagaga
    15121 tccggtaatg aattacccgg cgatggccca ggatcttgtt gataccctgg atgcactgca
    15181 gatcgacaaa gcaacattta tcggtcactc catgggcggt aaagcggtaa tggcacttac
    15241 tgcactggcc cccgatcgga ttgacaagct ggtggcaatt gatattgctc cggtcgatta
    15301 tcacgttcgc cggcatgatg agattttcgc ggccatcaac gcagtcagtg aatcggacgc
    15361 acaaactcgc cagcaagcag cggcaataat gcgccagcat cttaatgaag aaggggtgat
    15421 tcagtttctg ctgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc cggtattgtg
    15481 ggatcagtat ccgcatattg tgggttggga gaaaatcccg gcatgggatc accctgccct
    15541 gtttatccct ggcggcaatt ctccgtatgt tagcgagcag taccgcgatg atttactggc
    15601 acaatttcca caggcacggg cgcatgtgat tgcaggcgcg ggtcactggg tccatgctga
    15661 aaaaccggat gcggtattac gcgctatccg tcgctatctc aatgattaac gcaactgatt
    15721 aaaaacccag cgtccgcgtc gctttccgcg cgtgggcgtt ggcgtgactc atcgcgctga
    15781 tgtattatgg cgcgctatcc atccgggcag aggcctggct gattgtttcc cccgaagtca
    15841 ccaagatcat ggccaaagaa caaacggacc gtacgacatt agatctgttc gcgcacgagc
    15901 gtcgaccggg acgaccgaaa actaatccgc tttcgcgcga tgaacagctg cgtattaata
    15961 aacgcaacca gctaaaacgc gataaagtac gtggccttaa gcgtgtcgaa ctgaagctga
    16021 acgcggaagc tgtcgaggcg ctgaacgagc tggcggaatc gcgcaatatg agccgtagcg
    16081 aactgatcga agagatgtta atgcagcaac tggcggcgct gcgtagtcag ggcatcgttt
    16141 aaatttccac tttcatgtag cacagtgtgc agtcctgctc gtttgctgat tccgcaagac
    16201 tgcctgttct gctatgattg cctttatccg tgggcaattt tccaccccca tttcaataag
    16261 tttcaagagg ttatttcact catggctatc actggcatct ttttcggcag cgacaccggt
    16321 aataccgaaa atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt tgccgatgtc
    16381 catgacattg caaaaagcag caaagaagat ctggaagcat atgacattct gctgctgggc
    16441 atcccaacct ggtattacgg cgaagcgcag tgtgactggg atgacttctt tccgactctc
    16501 gaagagattg atttcaatgg caaactggtt gcgctgtttg gttgtggtga ccaggaagat
    16561 tacgctgaat acttctgtga cgcattgggc accatccgcg acatcattga accgcgcggt
    16621 gcaaccattg ttggtcactg gccaactgcg ggctatcatt tcgaagcatc aaaaggtctg
    16681 gcagatgacg accactttgt cggtctggct atcgacgaag accgtcagcc ggaactgacc
    16741 gctgaacgtg tagaaaaatg ggttaaacag atttctgaag agttgcatct cgacgaaatt
    16801 ctcaatgcct gatgtgatgc ggcgtagact catgtctacg ccgtattaat agataatgcc
    16861 aatcaaaata attgctacaa atttgtaact tttgctgttg tacctgtaca atgtcccggt
    16921 gttcaagtgg ccttgccgtt gtaaatgtaa gctgtgccac gtttttatta acaatatttg
    16981 ccagggactt gtggttttca tttaggcgtg gcaattctat aatgatacgc attatctcaa
    17041 gagcaaattc tgtcacttct cctaatgaag tgaaccgctt agtaacagga cagattccgc
    17101 atgactgata acaataccgc cctaaagaaa gctggcctga aagtaacgct tcctcgttta
    17161 aaaatcctgg aagttcttca ggagccggac aaccatcacg tcagtgcgga agatttatac
    17221 aaacgtctga tcgatatggg tgaagaaatt ggtctggcta cggtatatcg cgtactgaac
    17281 cagtttgacg acgctggtat cgtcacccgc cacaattttg aaggcggtaa atccgtattt
    17341 gaactgacac agcaacatca ccacgatcac ctgatctgcc tcgactgcgg caaggtaatc
    17401 gaatttagtg atgattccat cgaagcgcgt cagcgtgaaa ttgccgcaaa acatggcatt
    17461 cgcctgacta accacagtct ctatctttac ggtcactgtg ccgaaggcga ttgccgcgaa
    17521 gatgagcacg cgcacgaagg caaataagcc agccggaacg agaaaagcca acctgcgggt
    17581 tggctttttt atacacggga aataagaaaa gcgagagtta cagctctcac ttatttgtta
    17641 ttactgcgaa tttcctgcca gaccttatca cagtcggctt ttaccgcctg atcatttccg
    17701 gtttgcgttg cgcgcagaca ctgctgataa tccagtacgc gtacactttc ctggtcggca
    17761 aactgctgat gttgtttctc tttcttcagc acgtttaaca cgctctggca ggcttcaatt
    17821 ttttctggcg aaccttgtgc ggtgttgata caggcgctgt atgcctcttt cagacggctg
    17881 tcttcctgtg gcgcggttga ttgcgcacaa gccaccagcc ccgatgccat catggcgatg
    17941 agaatcagtt ttttcatagc aaattcctca aacgtgcggc aggcacaaaa cctgccgcgt
    18001 cgggcatcag aagatggtga atggtgcgat taccataaat ttcacgtcac gctcatcctg
    18061 gaagatgttg ccgtaaccac cgccccagct tgggatgtcg gagtggttgt cgtattcggt
    18121 gaagtgcagt ttgaacatcg tgcctttggc gcggccgtcc tgaatggtgt aaaccgcatc
    18181 caggctatat gcagactctt caatagtccg gttcttgtcg tagtacgcat ccggattgct
    18241 ctgccaggta gcgggtttag catcccatgc gtaaacgtag gaagcgccga tggcgaagcc
    18301 tggaagattc cagtttttca ggtcatacat cgcaccgaag aagaccgctt tttcgccgtt
    18361 agcgttgaag tcggaacggt tatcccacca gatatccagg cggccgttgg aggaagcgta
    18421 ggttggagtc atacgttgca ggaagtatcc ctgctgaccg tcagccttaa cccaggtgcc
    18481 ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca aaggtcaacg cttgcagcca
    18541 ggcggtgccg tcataaagat cgttgacgct gcgatcgtca actttatcgc gcgtaccata
    18601 gaactggtaa ctggtggtta acgggctacc ggcgatatca aatttgtagc tggctttggc
    18661 aaaatattga tcgatatacc cttccgcctg accaaatgcc gcttccagta cgaagttatt
    18721 tttgaagtcg tatttcgcac cgatggagtg aagataatca actttggtgg ttttatcgtt
    18781 ctgataaaac tcatccattt ccagatgcca cggtgctttg tattcgttgg tccacatgta
    18841 ggagaaactc aacgcaccag catcgccgta atcaaaattc gccccggctt ccgccccctg
    18901 ataagtaccc ggcataaagc tccagtgcgg cgctaacagc gtctgaccag ttggctgaat
    18961 gtaacctgcc ctcgcccaca ccggaccgta tttaaatttg gccgctgctt tatacaggct
    19021 tataccgctt ttgtcgccgg accagtcttc gtcataggct ttattgcttt ttgaaaacgc
    19081 gatttcgttc gggtgagagc tgtcaccgtt ttccgccatt tcaatcgccg taaacgcagc
    19141 aatatcaagg ccgaacatat cagcagcata accagactga aaatcgaggt tggcgttcca
    19201 ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt tacggtcgcg
    19261 ttcacgctgc cagtaataga taccgccggt taaggttgaa tcgtcgataa atccttcggc
    19321 gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcga tagccagcgc
    19381 cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga cgtataaatt
    19441 gctgcaccaa aggtgaacag cgaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    19501 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    19561 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    19621 gtttgcggtt gaaaggcttg cagaagaata aaagcgtgaa tttccagcaa gatgaaagcg
    19681 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    19741 gcaga
//
LOCUS       DAPOZO010000089        19374 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPOZO010000089 DAPOZO010000000
VERSION     DAPOZO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.089"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            274..426
                     /gene="hokA"
                     /locus_tag="ROI21_003534"
     CDS             274..426
                     /gene="hokA"
                     /locus_tag="ROI21_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HDX5112415.1"
                     /translation="MPQKYRLLSLIVICCTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            582..953
                     /gene="yidD"
                     /locus_tag="ROI21_003535"
     CDS             582..953
                     /gene="yidD"
                     /locus_tag="ROI21_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDX5112416.1"
                     /translation="MNKLLLLLIMLYQRHLSPRKGYRCAYSVLYQTQGCSGAVKEIIQ
                     QKGVIAGWGEIRQRFADCRLAAQTIRQKQHLNEVIRKKKNNNCDCSGCDGAVCRDCDI
                     PVPDLDCSWDCSSLHIKRFIQ"
     gene            complement(1007..1219)
                     /gene="cspA"
                     /locus_tag="ROI21_003536"
     CDS             complement(1007..1219)
                     /gene="cspA"
                     /locus_tag="ROI21_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDX5112417.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1500..1790)
                     /locus_tag="ROI21_003537"
     CDS             complement(1500..1790)
                     /locus_tag="ROI21_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDX5112418.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            2224..2934
                     /locus_tag="ROI21_003538"
     CDS             2224..2934
                     /locus_tag="ROI21_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDX5112419.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2984..3958)
                     /gene="ghrB"
                     /locus_tag="ROI21_003539"
     CDS             complement(2984..3958)
                     /gene="ghrB"
                     /locus_tag="ROI21_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDX5112420.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTIEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4062..4721)
                     /locus_tag="ROI21_003540"
     CDS             complement(4062..4721)
                     /locus_tag="ROI21_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDX5112421.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4874..7207
                     /gene="bisC"
                     /locus_tag="ROI21_003541"
     CDS             4874..7207
                     /gene="bisC"
                     /locus_tag="ROI21_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDX5112422.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVLSSRGALTTGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPEKPQGIRGQDEFVRVSWDEALELIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVNAEKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPKL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELSPFAEFWEANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYGYQDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDIVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7176..7616)
                     /locus_tag="ROI21_003542"
     CDS             complement(7176..7616)
                     /locus_tag="ROI21_003542"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDX5112423.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7613..8176)
                     /gene="tag"
                     /locus_tag="ROI21_003543"
     CDS             complement(7613..8176)
                     /gene="tag"
                     /locus_tag="ROI21_003543"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDX5112424.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEKNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            8334..9032
                     /locus_tag="ROI21_003544"
     CDS             8334..9032
                     /locus_tag="ROI21_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDX5112425.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            9261..10469
                     /locus_tag="ROI21_003545"
     CDS             9261..10469
                     /locus_tag="ROI21_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5112426.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10793..12484
                     /gene="eptB"
                     /locus_tag="ROI21_003546"
     CDS             10793..12484
                     /gene="eptB"
                     /locus_tag="ROI21_003546"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDX5112427.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            12576..12652
                     /locus_tag="ROI21_003547"
     tRNA            12576..12652
                     /locus_tag="ROI21_003547"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12610..12612,aa:Pro,seq:cgg)
     gene            12685..12820
                     /locus_tag="ROI21_003548"
     ncRNA           12685..12820
                     /ncRNA_class="other"
                     /locus_tag="ROI21_003548"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            13449..15056
                     /gene="dppA"
                     /locus_tag="ROI21_003549"
     CDS             13449..15056
                     /gene="dppA"
                     /locus_tag="ROI21_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDX5112428.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15207..16226
                     /gene="dppB"
                     /locus_tag="ROI21_003550"
     CDS             15207..16226
                     /gene="dppB"
                     /locus_tag="ROI21_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDX5112429.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16236..17138
                     /gene="dppC"
                     /locus_tag="ROI21_003551"
     CDS             16236..17138
                     /gene="dppC"
                     /locus_tag="ROI21_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDX5112430.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17149..18132
                     /gene="dppD"
                     /locus_tag="ROI21_003552"
     CDS             17149..18132
                     /gene="dppD"
                     /locus_tag="ROI21_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDX5112431.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLSDGRQSKCHYPLDDAGRPTL"
     gene            18129..19133
                     /gene="dppF"
                     /locus_tag="ROI21_003553"
     CDS             18129..19133
                     /gene="dppF"
                     /locus_tag="ROI21_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDX5112432.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19163..>19374)
                     /locus_tag="ROI21_003554"
     CDS             complement(19163..>19374)
                     /locus_tag="ROI21_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDX5112433.1"
                     /translation="TLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKVAQLEKL
                     RGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4556 a         4789 c         5109 g         4920 t
ORIGIN      
        1 aaataataca gcaaccttta ataatcttct gctgaataaa gattatctca tatattaatt
       61 ttatgagatt tttttaggat tatatcaagg agaagaaacg aacttattaa gctagaatag
      121 ccacgggtgc ttgagactgt ttgtctcagg tattcaccga aaggcagaca gagaaaagcc
      181 ccacctgact ataaatcaaa gtgaggctac cctatgcctg aacaccataa ggttagcctc
      241 ttactcgttg gaaatcaaca cagggggctg ggaatgccgc agaaatatag attactttct
      301 ttaatagtga tttgttgcac gcttttattt ttcacctgga tgataagaga ttcactgtgt
      361 gaattgcata ttaaacaggg gagttatgag ctggcggcat ttttagcctg caatttgaaa
      421 gagtaagagt ctttggcggg aagttattcc cgcctttctt acggcgttgc gcattctcat
      481 tgcacccaaa tttatttctt taatgaatgt ttttttaaat acgaatggat ttggctatta
      541 gataagtttt taatcgggaa caggaatggt tcggagcctt catgaataaa ctgcttttac
      601 tgttgataat gctctatcaa cgacaccttt ctcctcgcaa aggataccgc tgtgcttaca
      661 gtgtgttgta ccaaacgcag ggatgttcgg gagcagtcaa agagattatt caacagaaag
      721 gtgttattgc aggctggggc gagatacgtc agcgttttgc agattgccga cttgcggcac
      781 agacaatcag gcagaagcaa cacctgaacg aggtaatacg taaaaagaag aataataatt
      841 gtgattgttc cgggtgcgat ggtgcggtct gtcgtgattg cgacattccg gtgccggatc
      901 tggattgttc ctgggactgt tcttctctac atataaagcg gtttatccaa taaaaaaatc
      961 cccgccaaat ggcagggatc ttagattctg tgcttttaag cagagattac aggctggtta
     1021 cgttaccagc tgccgggcct ttagcaccgc tttcgatggt gaaggacact ttctgacctt
     1081 cgtccagaga tttgtaacca tcgttctgga tggcagagaa gtgtacgaac acgtctttag
     1141 agccatcgtc aggagtgatg aagccgaagc ctttgtcagc gttgaaccat tttacgatac
     1201 cagtcatttt accggacata gtgtattacc tttaataatt aagtgtgcct ttcggcgata
     1261 tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc actacgaggg gtatcaacga
     1321 taactcttga agggacttgc cttactacac tgctttaatg gtctgtacgt caaaccgttg
     1381 atgtgcatta agccacgcat tggcgggtga tgcaacaatt atttttcata tttatgatta
     1441 atcggttgga aaacggtcct gtcatcagga ccgtaaacag caataaagtg gataaaagtc
     1501 tattccatca actgcttact taatgccggg ttggcttgaa tcaaacgcat caattttagt
     1561 tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc gactgatacg
     1621 cccaaaaccc gggcgaaatc atcgattttt aaccctgttc cttttcgtaa ctgctcaatt
     1681 tcggtacagg atgttgttct gtgcgtcagg gtaattttct gcgtttcatc tttaaaaaca
     1741 atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat tgaaaactcc
     1801 tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag tcgggaaaag
     1861 cagacacagc ggggtgaagt cagggcatgc tctggctatg attcgattca gccagtcatt
     1921 tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct taccactccc
     1981 ggtttgtaaa gatttcgaaa atctaacgtt ctgagaaggc gtaaatctct ggcaacaaaa
     2041 aaagcaacgg cactatcatt ttttaacaat gaaacaacaa attaaccttg tttcattttc
     2101 tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg tcctggacta
     2161 tccttgtcac gtcagacacg tgtgtgtcgt tgtgcgcttt ttttgggtga aaggagtaag
     2221 aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc gttattaatg
     2281 gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc gtttatcgac
     2341 ttcctgcaga atacagtgat gcgtagcggt gaacgtctac caaccctgac tgccgatcag
     2401 aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta ttctcagcag
     2461 gtgaatcagg cgatggattc cggtctgcgc ccggtcgtag acagcgttaa cgcaattcgc
     2521 gtaccgcagg attacgttac gcaaagcggc ccactgcgtg aaatgaacgg ttctctgggc
     2581 gtactggcgc aacagctgca aaatgcgaag ttgcaggccg atgctgcgca ctctgcgcta
     2641 aaacagaccg atgatttgaa accggtcttt gatcaggcat tcaccaaagt ggtaacgacg
     2701 ccagctgatg cattgcaacc gttgattccg gcggcacaaa ctttcacgca acaactggtg
     2761 atggttggag actacattgc tcagcaaggt actcaggtga gctttgtcgc aaatggcatc
     2821 cagttcccga cgtcacagca ggcaagtgaa tataacaaac tgattgcgcc attaccggca
     2881 cagcatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca ataaagagta
     2941 aaagcccgag cgattatcgg gctttactcc acgcagtcgc ggcttagtcc gcgacgtgcg
     3001 gattcacaca gttcttctca acctttcctt gtaacgcatc aatcaaatta tccacggcac
     3061 aggcggccat gccataacgc gtctcatgag tggcagatcc aatatgcggt actgcgacga
     3121 cgttggccat tgagagcaac ggcgaatcta cagacagtgg ctcttgttcg aagacatcca
     3181 gcccggcagc gtgaatttcc cctttctgca atgcagcaat cagtgcattt tcatcaacca
     3241 ccgggccacg tccggcatta atgaatatgg cggaggattt cattttggcg aattgttctg
     3301 cgccaaacag atgatgcgtt tcatcagtta acggcaggat caggcaaacg aaatctgact
     3361 cttgtaacag tgtatccaaa tcgcagtagc gggcgttgaa gcgttcttct gcttctttat
     3421 ggtggcggcg cgcgttatag aggatgggca tgttgaaacc aaagtgcgca cgttgtgcca
     3481 gcgccatacc gatccgtccc atcccgacaa tgcccagtgt tttatggtga acgtcagtgc
     3541 cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt tctgctacct
     3601 ccacaacccg acgagcggta gacaacacca gcgccatcag cgtatcggcg acggtttctg
     3661 ttaatacggt tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca tcgacatcaa
     3721 aattgtcata gccgacggag atcgttgatg tggcacgcag tttcggcatt ttttccagca
     3781 atgcggcatc aacattctcg tttgaaccca gtaaaccttc agcttcggca aaaattgctg
     3841 cattttgttc gatggtttgt gggctgaggt ttgccacctg gtgaacggtg aaatgctctt
     3901 gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac ggcttcatgc
     3961 ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa ggaaaactga
     4021 agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg gcttaaggta
     4081 atttctacac ggcggttttg cgccttacct tctgcggtgc tgttgctggc gattgggtta
     4141 gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt gatcaacgcg
     4201 ctggcaacgg aatccgcacg ctgctgggag agacgcatgt tcaggtcgtg accacccgtg
     4261 ctgtcggtat aaccaatcac gttaaccgcc gttttcggat actctttcag caccattgcc
     4321 acgccggtca gggtgttagc gcccgccggt ttcagggtcg cgctgctgct gtcgaaggtc
     4381 acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct aacaccagtg
     4441 ccgcgcattt tgtcacgtag cttcgcttcc tgcacatcca tgtaataacc cacgccgccg
     4501 cccagagctg cgcctgctgc tgcgccaatg agcgcgcctt taccgcgatc tttcttcgaa
     4561 gaagagagcg caccaatacc cgcgcccacg agagagccca gacctgcgcc aatagcagat
     4621 ttacctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac cgccagagcg
     4681 ccactcacta ctgcggcaat aagataaaca cgtttcttca ttgttaatcc ttaataacct
     4741 ttttattctt tgccacgggt tccgtggcgg gagattatgc cgcgtgaaca tgaagattat
     4801 tcctgggaat actcggaaat ttgtaagtaa tatttaactg ctcaatacat ctaacctttc
     4861 aggagtcttc ggtttggcca actcatcctc acgatattcc gttctgactg ccgcccactg
     4921 ggggcccatg ctggttgaaa ctgacggcga aaccgtgctt agctcgcgtg gcgcgttaac
     4981 cacaggaatg gaaaactcct tgcagagcgc ggttcgcgac caggttcaca gcaatacgcg
     5041 ggtacgattt ccaatggtgc gcaaaggctt tcttgcgtca ccggaaaaac cgcaaggcat
     5101 tcgcgggcag gatgaatttg ttcgtgtgag ttgggatgag gcgctggagc ttattcacca
     5161 acaacataaa cgcattcgtg aggcttatgg tccggcatcg atttttgctg gttcctacgg
     5221 ctggcgttca aacggcgtgc tgcataaggc ctcgacatta ttacaacgct atatggcgct
     5281 ggcaggcggt tataccgggc atctggggga ttattcgacc ggtgcagcac aggcgatcat
     5341 gccgtatgtc gtggggggca gcgaagttta tcaacagcaa accagttggc ccctggtgct
     5401 ggaacatagc gatgtcgtgg tgctgtggag tgctaatcca ctcaatacgc tgaaaattgc
     5461 gtggaatgca tccgatgagc aggggctttc ttacttttcc gcactgcgtg acagcgggaa
     5521 aaagctgatc tgcattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat
     5581 ggagtgggtg gcaccgcaca tgggcaccga tgttgcgctg atgctgggga tcgcccatac
     5641 gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca ccggttatgc
     5701 cgtcttcgcc tcttatttgc tgggcgagag tgacggaata gccaaaaccg ccgaatgggc
     5761 ggcagggatt tgtggagtta acgcagagaa aatccgcgag ctggcggctc ttttccacca
     5821 aaacacgacc atgctgatgg ctggctgggg aatgcagcgt caacagtttg gtgagcaaaa
     5881 acactggatg atcgtcacgc tggcggcaat gttggggcaa atcggcacac ccggcggcgg
     5941 ttttggtctt tcttatcatt ttgccaatgg tggtaacccc acgcggcgtt ctgcggtgct
     6001 ctcttccatg cagggcagct tgcctggcgg caccgatgcg gtagataaaa tccctgttgc
     6061 ccgcattgtt gaagcactgg aaaaccctgg tggcgcatat caacacaacg gtatgaaccg
     6121 acatttcccg gatattcgtt ttatctggtg ggcgggcggt gccaacttta ctcatcatca
     6181 ggataccaat cgcctgatcc gtgcctggca aaaaccgaag ctggtggtga tctctgaatg
     6241 cttctggacg gctgcggcga aacacgcgga tatcgttctg cctgcgacta cctcttttga
     6301 gcgtaatgat ctcaccatga ccggtgatta cagtaatcag catctggtgc cgatgaaaca
     6361 agtggtgccg ccacgttatg aagcgcgtaa tgattttgat gtctttgccg agttgagtga
     6421 acgttgggag aagggcggct atgcgcggtt tacggaagga aaaagtgagc tgcaatggct
     6481 ggaaacgttt tataacgttg cccggcagcg cggggcaagc cagcaggttg aattgtcgcc
     6541 atttgctgag ttctgggaag ccaaccagtt aattgagatg ccggaaaacc cggacagtga
     6601 gcggtttatt cgcttcgccg attttcgccg cgatccgcag gcgcatccat taaaaaccgc
     6661 cagcggtaag attgaaatat tctcgcagcg tattgccgat tatggttacc aggattgccc
     6721 tgggcatccg atgtggctgg agccggatga gtggcagggc aatgccgaac cagaacagtt
     6781 gcaggtactt tctgctcatc cggcacatcg tctgcacagc cagctgaatt acagttctct
     6841 gcgcgaattg tacgcggtgg caaatcgtga gcctgtcacc attcatcctg acgatgccca
     6901 ggcgcgcggc atacaagatg gcgatattgt tcggttgtgg aacgcacgcg ggcaaattct
     6961 tgccggagcg gtcattagcg agggaattaa acctggcgtg atttgtattc atgaaggggc
     7021 atggccggat ctggatttaa ccgctgacgg tatttgtaaa aacggcgcgg tgaacgttct
     7081 gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata cggcactggc
     7141 atggctggaa aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag
     7201 ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcagtcgac aatgtgaaaa
     7261 ccctgcgcat ggtaaaaatc tatcgccggt tgattttttt gataaacctc cagcatcaga
     7321 tggggatggc gctgctgcac atactgcatt agcgccttac caataccgcg tctgacggcc
     7381 ttcggtgcga caaacattgc agccagaaat cggccttcca taatgctgac aaaaccgaga
     7441 agcttaccgt cttcttccca gacccagttt tgcgcgttgg caagataggc atcccgcacc
     7501 agcggaatgc aatcacgcca gtaattcgct tttataaagg gatgccccca ggttgtactt
     7561 tccagccaca gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg aatcatggtt
     7621 tatttcccgg atagcaacaa cagccaacca catgatcatt caccagccca catgcctgca
     7681 taaaggagta acagattgtg gtaccaacaa acttaaaacc acgctttttc agggctttag
     7741 atagagcgtc ggaggcgggc gtagatgtag ggatttcgct caacgttgtg gcttgtgtca
     7801 cctgtggttg atgattgaca aacgaccaga caaagtcggc aaacggttcg ccgttctttt
     7861 ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttcccg cgatggcgga
     7921 taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgca gccaccttca
     7981 ccggatcgaa ctgatgaaag ctggcgcgat agttttcgcg ttttttgagg acggtgatcc
     8041 acgataatcc agcctgctgc ccttcaaggc agatcatttc gaacagtttt ttactgtcag
     8101 tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggtcc tgactcaccc
     8161 agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt
     8221 ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct
     8281 tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca
     8341 taaaaaaaag cggtggtcgc tggcagctaa gcctgctggc gagtgtggta atcagtgcct
     8401 tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg caacccggac
     8461 attccacaga gcgttacacc tgggatagtg atcaccaacc tgattacaac gatattttgt
     8521 cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa
     8581 ctcctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgagcagg
     8641 ttacaaccgg cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg
     8701 agtttggcga cagtactacc acgctggccg atccgttatg gcatgccagc gtgagtacct
     8761 taggctggcg tgttgactcc cggcttggcg atctccgacc ctgggcgcaa atcagctata
     8821 accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga
     8881 caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa
     8941 atattgctgc ctatgctgcg ctatctcagg cagaaaatac cactaacaat agcgactatc
     9001 tgtatacgat gggggttagt gccagatttt aacgttatag atacaattga aaccattgga
     9061 taacaacagt tgtggcgata gtgggtgcta acttactaaa taataaattt ggtgaataat
     9121 tgtcgagtca ttcattcctg aactaatgca tttcattccg ttctgatggc atttcatgcc
     9181 gtttttcccc aggcataaag tgcacttcgt tatggttgtc agcagagatt tttccttttt
     9241 attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc tggttgacac
     9301 tcatcggtac tatcattacc cagtttgcgc tggggtcggt ttatacctgg agcctgttta
     9361 acggcgcgct ttctgccaag ctggatgcgc cggtaagcca ggtcgctttc tctttcggct
     9421 tgttaagtct ggggctggca atttcatctt ctgttgcggg gaaattacag gaacgttttg
     9481 gcgttaaacg cgtcactatg gcttccggca ttttgctggg attaggcttc ttcctgacag
     9541 cgcattctga caacctgatg atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg
     9601 atggcgcggg ttacctgctg acgctctcta actgcgtgaa gtggtttccg gagcgtaaag
     9661 gtctgatctc cgcgttcgct atcggttctt atggtctggg tagcctgggt ttcaaattta
     9721 tcgacacgca gctgctggaa acggtgggtc tggaaaaaac atttgtgatt tggggagcga
     9781 ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag
     9841 tgaaaaccag caatggtgtc gtggagaaag attacacgct ggcagagtcg atgcgtaaac
     9901 cgcagtactg gatgttagcg gtaatgttcc tgactgcctg catgagcggc ctgtacgtga
     9961 ttggggtagc gaaagatatc gcccaaagtc tggcacacct tgatgtggtt tccgcagcca
    10021 atgcagtcac tgttatttcc atcgccaacc tttcaggtcg tctggttctg gggatactgt
    10081 ctgacaaaat cgcccgaatc cgtgttatca ccattggtca ggtgatttcg ctggtgggta
    10141 tggcggccct gctgtttgca ccattgaatg cagtgacgtt ctttgcagcg attgcctgcg
    10201 tggcgtttaa ctttggcggc actatcacgg tgttcccgtc actggtcagt gagttcttcg
    10261 gcctcaataa cctggcgaaa aactacggtg tgatttatct cggtttcggt atcggcagca
    10321 tttgtgggtc gattatcgcc tcactgtttg gcggcttcta tgtgaccttc tacgtgattt
    10381 tcgccctgct gattctgtca ctggcgctct ctacgacgat tcgtcagcca gagcagaaaa
    10441 tgttgcgtga ggcgcatggc tccctttaat catctgccgc gagctactgg gctttagcct
    10501 tagtgtgaat caaacgcttc aacgcaatgt ataaacaatg acgcgtaaaa tttgggccag
    10561 ttaactggcc ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt
    10621 tgtaaatttg tgcttcgtgc acactctttc cccacacttt ttccctttgc tgtggtctac
    10681 ttattcgcgc gtgtagattt tacttatctg actacctccg cacttttccc ctgcctggcc
    10741 tgaaaagcca ttaagcaggg tgttatcacc tgtttgtcca gggtttgctt gcatgagata
    10801 catcaaatcg attacacagc agaagctgag ctttttgctt gcaatctata ttggcctttt
    10861 tatgaatggc gcggtttttt accgccgctt cggcagctat gcgcacgatt ttaccgtctg
    10921 gaaaggcatt tctgctgttg ttgaactggc cgccaccgta ctggtgacct tctttttact
    10981 acgtcttctt tcgctgtttg gccgccgcag ttggcgtatt ctggcatcgc tggtggtgct
    11041 cttttccgcc ggtgccagct attacatgac cttccttaat gtggtcattg gttatggcat
    11101 catcgcttcc gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt
    11161 tattctctgg ttaatcgccg ttagtgcatt gcctcttatc cttatctgga ataaccgctg
    11221 tcgctacacc ttgctccgac aactgcgaac cccagggcag cgtattcgca gcctggcggt
    11281 cgtcgtactg gcgggtatta tggtttgggc accgattcgt ttgctggata tccagcagaa
    11341 gaaagtggag agggcgaccg gcgttgattt gccgagttat ggcggtgtcg tagcgaactc
    11401 ttatctgcca tcaaactggc tttctgcgtt ggggctgtat gcctgggcgc gggtcgatga
    11461 atcttccgat aataattcat tgcttaatcc ggcgaagaaa ttcacttatc aggcaccgca
    11521 aaacgttgat gacacttatg tcgtgtttat cattggtgaa accacgcgtt gggatcatat
    11581 gggcattttc ggctatgaac gcaataccac accgaaactt gctcaggaga aaaatctggc
    11641 ggcgttccgt ggttactcct gtgatactgc aaccaaactc tcactgcgtt gcatgtttgt
    11701 acgtcagggt ggagcagaag ataatccgca gcgcacatta aaagaacaga acattttcgc
    11761 ggtattgaag cagttaggat tcagttctga tctctacgct atgcaaagcg aaatgtggtt
    11821 ctacagcaat acgatggcgg acaacattgc ttatcgtgag cagattggtg cggagccacg
    11881 taaccgtggc aagccggtag atgatatgtt gctggtagac gaaatgcagc aatcgctggg
    11941 gcgcaacccg gatggtaagc atctgatcat tctgcatacc aaaggctcac actttaacta
    12001 cacccagcgt tacccgcgca gctttgcgca gtggaagccg gaatgtattg gtgttgatag
    12061 cggctgtacc aaagcgcaga tgatcaactc atacgacaac tcggtgacct atgtggatca
    12121 ctttatctcc agcgtgattg atcaggttcg cgataagaaa gcgattgtgt tctacgcagc
    12181 tgaccacggt gagtcaatta atgaacgcga gcacctgcac ggcacgccgc gtgaactggc
    12241 accgccggag cagttccgcg tgccgatgat ggtctggatg tcagataaat atctggaaaa
    12301 tccggccaat gcgcaggcat ttgcgcagct gaaaaaagaa gccgacatga aagtgccacg
    12361 ccgtcatgta gagctgtacg acaccatcat gggttgtctt ggctatactt caccggatgg
    12421 tggaattaac gaaaacaaca actggtgcca catcccgcag gcaagagagg cggcggctaa
    12481 ctaagaggcc tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa gggattgacg
    12541 agggcgtatc tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag cctggtagcg
    12601 cacttcgttc gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa
    12661 atgcctgctc agcgagcagg cttttttgca tttgtagctc atgaggatga gaacctccgg
    12721 gggcggggag gttcgactcg agcgaagcga gagaacgttg cgccagcaac ggcccgcagg
    12781 gcgagccacg aagtggcgag taatcctctg gcaccgacca aattcggcac cgcactcatt
    12841 gtgggataca gcgtatccgt cttatccgat ctaaacggca acactccaca catttccctg
    12901 ccaaatttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
    12961 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
    13021 ctgtgcggtt tagcgttcat ggtattaatc gctcagatat ccaccttttc atcagatttt
    13081 tttcacaaaa tgcataaaac ttaatcacca attgttgcta attattaagc tgattgttct
    13141 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ttgtttgaag tttttttaat
    13201 ctttgtttgt ggattctcac cgttggtgta aatcccggtg ggctgtattg acgttttcac
    13261 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgtaacac
    13321 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    13381 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    13441 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    13501 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    13561 ctccggaagg gtttaacccg cagttgttta cctccggtac tacctatgac gcctcttccg
    13621 tgccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg attccgggcc
    13681 tcgctgaaaa gtgggaagtc agcgaagacg gaaaaaccta taccttccat ctgcgtaaag
    13741 gtgtgaagtg gcacgacaac aaagaattca aaccgacgcg cgaactgaac gccgatgacg
    13801 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
    13861 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13921 tggacgacaa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13981 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    14041 gtacaccgga aaaactggac ctcaacccaa tcggtaccgg tccgttccag ttacagcagt
    14101 atcaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    14161 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    14221 agaagaatga atgccaggtg atgccgtacc cgaacccggc agatatcgct cgcatgaagc
    14281 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctat ctttcatata
    14341 acgtgcagaa aaaaccactc gatgatgtga aagttcgcca ggctctgacc tacgcggtga
    14401 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg cgtggcagcg aaaaacctga
    14461 tcccgccaac catgtggggc tataacgacg acgttcagga ctacacctac gatcccgaaa
    14521 aagcgaaagc cttgctgaaa gaagcgggcc tggaaaaagg cttctccatc gacctgtggg
    14581 caatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    14641 cagactgggc gaaagtcggc gtgcaggcca aaattgtcac ctacgaatgg ggtgagtacc
    14701 tcaagcgtgc gaaagatggc gagcaccaga cggtaatgat gggctggact ggcgataatg
    14761 gggatccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggctcca
    14821 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14881 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14941 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagttaaag
    15001 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    15061 aagccatacc cggtggcgct gtgcctctgc gtgcagcgcc atccggcagc aatacttcat
    15121 tccctgcccg acctgggcgg gaattgattt gtgagcaata cagacacgca gttccaggct
    15181 gcgggtcact acagagaatc cgggttatgt tgcagtttat tctccgacgt ttgggactcg
    15241 tcatccccac gtttatcggt attacccttc tcacatttgc ctttgtccac atgatcccgg
    15301 gcgatccggt gatgatcatg gcgggcgaac gtgggatctc cccagagcgt cacgcgcagc
    15361 tgctggctga actcggctta gataaaccga tgtggcagca gtatctccat tacatttggg
    15421 gcgtaatgca cggcgattta ggcatttcga tgaaaagccg catcccggta tgggaagagt
    15481 tcgtgccgcg ctttcaggcc acgctggaac ttggcgtctg cgcgatgatt tttgctacgg
    15541 cagtcggtat tccggttggt gtgctggctg cggttaaacg cggttccatt ttcgatcaca
    15601 cggcggttgg cctggcgctg acaggttatt caatgcctat cttctggtgg ggcatgatgc
    15661 tgatcatgct ggtttcggtg cactggaacc tgacgcccgt ctccggtcgc gtgagcgaca
    15721 tggtgttcct cgatgattcc aatccgttaa ccggttttat gctaatcgac accgccatct
    15781 ggggtgaaga cggtaacttt atcgatgccg tcgcccatat gatcctgccc gccattgtgc
    15841 tgggtactat cccgctggcg gtcattgtac gtatgacacg ctcctcgatg ctggaagtgc
    15901 tgggtgaaga ttatatccgc accgcgcgcg ccaaagggtt gacccgcatg cgggtgatta
    15961 tcgtccatgc gctgcgtaac gcgatgctgc cggtagtgac cgttatcggc ctccaggtgg
    16021 gaacattgct ggcgggggcg attctgaccg aaaccatctt ctcgtggccc ggtctggggc
    16081 gctggttgat tgacgcactg caacgccgcg actatccggt agtgcagggc ggcgtattgc
    16141 tggtggcgac gatgattatc ctcgtcaact tgctggtcga tctgctgtac ggcgtggtga
    16201 acccgcgtat tcgtcataag aagtaagggg acatcatgtc acaggttact gaaaataaag
    16261 tgattagcgc accggtgccg atgaccccgt tacaggagtt ctggcactat tttaaacgca
    16321 acaaaggcgc ggtcgtcggg ctggtttacg tcgtcatcgt gctgtttatc gcgatctttg
    16381 ccaactggat tgcaccctat aacccggcgg aacagttccg cgatgcactg ctcgccccgc
    16441 cagcctggca ggaaggcggc agcatggcgc acttgctggg caccgatgac gtaggccgtg
    16501 atgtgctgtc gcgtctgatg tacggtgcgc gattgtcact gctggttggc tgtctggtag
    16561 ttgtgttatc gctgattatg ggcgttattc tcggcctgat cgccggttac ttcggaggcc
    16621 tggtcgataa catcatcatg cgcgtggtcg atatcatgct ggcgctgcca agtttgctgt
    16681 tggcactggt gctggtggca attttcggcc cgtcgattgg taacgccgcg ctggcattga
    16741 ccttcgttgc cttgccgcac tatgtgcgct taacccgcgc cgccgtgctg gtggaggtga
    16801 atcgcgatta cgttaccgcg tctcgcgtgg caggtgccgg ggcgatgcgt cagatgttta
    16861 ttaacatctt cccgaactgc cttgcgcctc tgattgttca ggcgtcgctc ggtttctcta
    16921 acgccattct cgatatggct gctcttggtt tcctcggcat gggggcgcag ccgccaacgc
    16981 cggagtgggg caccatgctc tccgacgtgt tgcagttcgc gcaaagcgcc tggtgggtcg
    17041 tgaccttccc gggtctggcg atcctgctga cggtgctggc atttaacctg atgggtgacg
    17101 gtctgcgtga cgcgctcgat cccaaactga agcagtaaga ggttcgagat ggcgttatta
    17161 aatgtagata aattatcggt gcatttcggc gacgaaagcg caccgttccg cgccgtagac
    17221 cgcatcagct acagcgtaaa acagggcgaa gtggtcggga ttgtgggtga gtccggctct
    17281 ggtaaatcgg tcagttcgct ggcgattatg gggctgattg attatccggg ccgcgtaatg
    17341 gcggaaaagc tggagtttaa cggtcaggat ttacagcgta tctcagaaaa agagcgccgc
    17401 aacctggtgg gtgccgaagt ggcgatgatc ttccaggacc cgatgaccag ccttaacccg
    17461 tgctacaccg tgggtttcca gattatggaa gcgattaagg tgcatcaggg cggcaacaaa
    17521 agtacccgcc gtcagcgggc gattgacctg ctgaatcagg tcggtattcc cgatccggca
    17581 tcgcgtctgg atgtttaccc gcatcagctt tccggcggca tgagccagcg cgtgatgatc
    17641 gccatggcga ttgcctgtcg accaaaactg ctgattgccg atgagccgac cactgcgctg
    17701 gacgtgacca ttcaggcgca aatcatcgaa ctgttgctgg agctacagca gaaagagaac
    17761 atggcgctgg tgttgattac ccatgacctg gcgctggtgg cggaagcggc acataaaatc
    17821 atcgtgatgt atgcaggcca ggtggtggaa acgggcgatg cccacgccat cttccatgcg
    17881 ccgcgtcatc catatactca ggcattgctg cgtgcgctgc cggaatttgc tcaggacaaa
    17941 gaacgtctgg cgtcgttgcc aggtgttgtt cccggcaagt atgaccgccc gaacggctgc
    18001 ctgcttaacc cgcgctgccc ctatgccact gacagatgtc gcgctgaaga accggcgctg
    18061 aatatgctct ctgacgggcg tcagtccaaa tgccattacc cacttgatga tgccgggagg
    18121 ccgacactat gagtacgcat gaggccacct cgcaacaacc gctgttgcag gctatcgacc
    18181 tgaaaaaaca ttatccggtg aagaaaggta tgttcgcgcc ggaacgtctg gtgaaggcgc
    18241 tggacggcgt ttcgtttaac cttgaacgtg gcaaaacgct ggcagtagta ggtgaatctg
    18301 gctgcggtaa atcgaccctc ggtcggttgc tgacgatgat cgaaatgccc accggtggtg
    18361 agctgtatta ccaggggcag gatctgctca agcacgatcc gcaggcgcag aagctgcgtc
    18421 ggcagaaaat ccagattgtc ttccagaacc cttacggttc gctgaatcca cgtaaaaaag
    18481 tcgggcaaat tcttgaagag ccgctgctta ttaataccag cttaagcaaa gaacagcgtc
    18541 gggaaaaagc cctgtcgatg atggcgaaag tcggcctgaa aaccgaacat tatgaccgct
    18601 atccgcatat gttctccggc ggtcagcgtc agcgtatcgc cattgcccgt ggtctgatgc
    18661 tcgacccgga tgtggtgatt gccgatgaac cggtttccgc gctggacgtt tcagtgcgtg
    18721 cgcaggtgct gaatctgatg atggatttgc agcaggagtt ggggctgtct tatgtcttta
    18781 tctcgcacga cctgtcagtg gttgagcaca ttgccgatga agtgatggtg atgtacctgg
    18841 gccgctgcgt ggagaaggga acgaaggacc aaatcttcaa taacccgcgt catccgtaca
    18901 ctcaggcgct actctccgcg acgccgcgcc tgaacccgga cgatcgccgc gagcgcatca
    18961 agctcaccgg tgaactgcca agcccgctca atccaccgcc gggctgcgcg tttaacgccc
    19021 gctgtcgtcg gcgcttcggc ccctgcactc agttgcagcc gcagctaaaa gactacggcg
    19081 gtcaactggt agcttgtttt gctgttgatc aggatgaaaa tccgcagcgt taactcacaa
    19141 aaccggggcc atgcgctccg gtttactcaa taagcttcaa cagtggcgac aagcataaca
    19201 aaatgccgta cagcagaatc agccaggctt taaacccgcg aagtttttcc agttgtgcga
    19261 ctttatagat caggaaaaat ggaatgaggc acgacacaat gccatataac gggcttccca
    19321 actgaaagaa caccagcacc gatacacgaa acgaaaccca aatcgtcaac gtaa
//
LOCUS       DAPOZO010000090        18603 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPOZO010000090 DAPOZO010000000
VERSION     DAPOZO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.090"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(8..352)
                     /locus_tag="ROI21_003555"
     CDS             complement(8..352)
                     /locus_tag="ROI21_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDX5112434.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            504..833
                     /locus_tag="ROI21_003556"
     CDS             504..833
                     /locus_tag="ROI21_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDX5112435.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARGKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1081..1326
                     /gene="nrdH"
                     /locus_tag="ROI21_003557"
     CDS             1081..1326
                     /gene="nrdH"
                     /locus_tag="ROI21_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDX5112436.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1323..1724
                     /gene="nrdI"
                     /locus_tag="ROI21_003558"
     CDS             1323..1724
                     /gene="nrdI"
                     /locus_tag="ROI21_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDX5112437.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNNEHNRVLLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQP"
     gene            1706..3850
                     /gene="nrdE"
                     /locus_tag="ROI21_003559"
     CDS             1706..3850
                     /gene="nrdE"
                     /locus_tag="ROI21_003559"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDX5112438.1"
                     /translation="MATTTVERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     ITTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFTDRVTMVALTLAQGDETLAAQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYAEL
                     VANERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERSETFAGFKQSRYANGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3860..4819
                     /gene="nrdF"
                     /locus_tag="ROI21_003560"
     CDS             3860..4819
                     /gene="nrdF"
                     /locus_tag="ROI21_003560"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDX5112439.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLLPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISQAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5176..6378
                     /gene="proV"
                     /locus_tag="ROI21_003561"
     CDS             5176..6378
                     /gene="proV"
                     /locus_tag="ROI21_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDX5112440.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6371..7435
                     /gene="proW"
                     /locus_tag="ROI21_003562"
     CDS             6371..7435
                     /gene="proW"
                     /locus_tag="ROI21_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDX5112441.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            7493..8485
                     /gene="proX"
                     /locus_tag="ROI21_003563"
     CDS             7493..8485
                     /gene="proX"
                     /locus_tag="ROI21_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDX5112442.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINVQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            8938..10122
                     /locus_tag="ROI21_003564"
     CDS             8938..10122
                     /locus_tag="ROI21_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5112443.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10246..10983
                     /gene="ygaZ"
                     /locus_tag="ROI21_003565"
     CDS             10246..10983
                     /gene="ygaZ"
                     /locus_tag="ROI21_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDX5112444.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            10973..11308
                     /gene="ygaH"
                     /locus_tag="ROI21_003566"
     CDS             10973..11308
                     /gene="ygaH"
                     /locus_tag="ROI21_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDX5112445.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAIGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11399..11929
                     /gene="emrR"
                     /locus_tag="ROI21_003567"
     CDS             11399..11929
                     /gene="emrR"
                     /locus_tag="ROI21_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDX5112446.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12056..13228
                     /gene="emrA"
                     /locus_tag="ROI21_003568"
     CDS             12056..13228
                     /gene="emrA"
                     /locus_tag="ROI21_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDX5112447.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13245..14783
                     /gene="emrB"
                     /locus_tag="ROI21_003569"
     CDS             13245..14783
                     /gene="emrB"
                     /locus_tag="ROI21_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDX5112448.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(14847..15362)
                     /gene="luxS"
                     /locus_tag="ROI21_003570"
     CDS             complement(14847..15362)
                     /gene="luxS"
                     /locus_tag="ROI21_003570"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDX5112449.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(15512..17068)
                     /gene="gshA"
                     /locus_tag="ROI21_003571"
     CDS             complement(15512..17068)
                     /gene="gshA"
                     /locus_tag="ROI21_003571"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDX5112450.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGRLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDIAGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSRELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(17141..17569)
                     /locus_tag="ROI21_003572"
     CDS             complement(17141..17569)
                     /locus_tag="ROI21_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDX5112451.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(17566..18132)
                     /gene="yqaB"
                     /locus_tag="ROI21_003573"
     CDS             complement(17566..18132)
                     /gene="yqaB"
                     /locus_tag="ROI21_003573"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDX5112452.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGILAARAAGMDAVDVRLL"
     gene            complement(18343..18419)
                     /locus_tag="ROI21_003574"
     tRNA            complement(18343..18419)
                     /locus_tag="ROI21_003574"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18383..18385),aa:Arg,seq:acg)
     gene            complement(18522..18598)
                     /locus_tag="ROI21_003575"
     tRNA            complement(18522..18598)
                     /locus_tag="ROI21_003575"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18562..18564),aa:Arg,seq:acg)
BASE COUNT         4448 a         4836 c         4734 g         4585 t
ORIGIN      
        1 cgcgtcttta actggcttcg ccaaataaac cattcaaata acgttcaagc gcaacacgcg
       61 aactaaagcc atgcgggata ccgtaatgct catgacgttc acccgctaaa tagagcggaa
      121 attgttgata atgatcgcaa gttttaatat cggaagcggg ttcagccagt gttgaactac
      181 gttcttttaa tgttggatga atcaccaggg cggtacgtcc cattcgcgct tcgcgattaa
      241 catagacata attttcccca cggcggtaac cataagcttt ctgggttacc acatcgacag
      301 taaaaccgac tttttcaagt acgcgcgcca cctcgtctgg tcgtaaatac atatttgatc
      361 ctcgttatta tcaaccgcgg gcctacctta cctgattgcg cattcacaac gctttcagaa
      421 aagtccataa agccgcgatg aacctcagtt aagaaaatta tggtctacac tgaaaattac
      481 atcgaattct aatgggggat catatgttta accgaccgaa ccgcaacgac gttgatgatg
      541 gcgtgcagga tatccagaat gatgtcaatc aattagctga cagtctcgaa tctgtattga
      601 agtcctgggg cagcgacgcc aaaggggaag ccgaagccgc tcgtggtaaa gcgcaggctc
      661 tactgaaaga aaccagggcg cgaatgcatg gtcgtactcg cgtccagcaa gccgcgcgcg
      721 atgccgttgg ctgcgcggat tcttttgttc gtgaaagacc ctggtgtagc gtgggtacag
      781 cagctgcggt aggtattttt atcggcgcac tgttaagcat gcgcaaatcg tagtgcaata
      841 atgataataa atacgcgtct ttgaccccga agcctggctt cggggtttct ttttgcctgg
      901 tgaatcacaa aaatccccct accccgtcac gctcataccc agggtaattt cgaccactat
      961 ttgctatata ttgtgtggtt gaatcttttt tcaactacat ctagtatctc tgtatcaaca
     1021 gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat tttaaacgga aatacgaatc
     1081 atgcgcatta ctatttacac tcgtaacgat tgcgttcagt gccacgccac caaacgggcg
     1141 atggaaaacc ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcggca
     1201 gaagcgctgc gtgctcaggg ctttcgtcag ttgccggttg tgattgcagg cgatcttagc
     1261 tggtctggct tccgcccgga catgatcaac cgtctgcatc cagcaccaca cgcggccagt
     1321 gcatgagcca gctcgtctac ttctccagta gctccgaaaa tacgcagcgt tttatcgaac
     1381 gtttaggact gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg
     1441 agccttacat cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcagtgccac
     1501 gacaggtcat tcgcttttta aacaacgagc acaaccgggt gttgctgcgc ggcgttattg
     1561 cttcgggtaa tcgcaacttt ggtgaggcgt atggccgcgc cggagatgtg attgcccgga
     1621 aatgcggcgt gccgtggctg taccgctttg aactcatggg tacgcaaagc gatatcgaaa
     1681 acgttcgtaa aggagtaacc gaattttggc aacgacaacc gtagaacgcc tgacgcagga
     1741 aacgatggat tatcacgctc tgaatgcgat gcttaacctt tacgatagcg caggtcgcat
     1801 tcagttcgat aaagatcgcc aggcagtaga cgcctttata acgacgcatg tgcgtccgaa
     1861 cagtgtggcc ttcagtagcc agcagcagcg cctgaactgg ctggttaacg aaggttacta
     1921 tgatgaaagc gtccttaatc gctactctcg cgattttgtc attacgctgt ttgcccacgc
     1981 gcacgccagc gggtttcgtt tccagacatt cctcggagca tggaagtttt acaccagcta
     2041 tacgttgaag acattcgacg gtaagcgtta tctggaagat tttaccgatc gcgtcacgat
     2101 ggtggcgctg acgctggcac aaggcgatga aacactggcg gcgcagctca ctgatgagat
     2161 gctgtcagga cgctttcagc cagccacgcc gacattcctc aactgcggta agcagcagcg
     2221 cggcgaactg gtttcctgtt ttctactgcg tattgaagac aatatggagt cgattggccg
     2281 ggcggtaaat tccgcactgc aactgtcgaa acgcggcggc ggcgtggcat ttttgctgtc
     2341 gaatctgcgg gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtgat
     2401 tccggtgatg aaaatgctgg aagacgcatt ttcctatgcc aaccagctcg gcgcgcgtca
     2461 gggtgctggc gcagtctatt tacatgccca tcatcccgat attctgcgtt ttctcgatac
     2521 gaaacgggaa aatgccgacg agaaaatccg cattaaaaca ctgtcgctgg gcgtggtgat
     2581 cccggatatc actttccatc tggcaaaaga gaatgcgcag atggcactgt tttcgcctta
     2641 tgacgttgag cgggtttatg gcaagccgtt tgctgatatc gccatcagtg aacactatgc
     2701 cgaactggtt gccaatgaac gcatccgcaa aaaatacctc aacgcccgtg atttcttcca
     2761 gcgactggca gaaatccagt ttgagtccgg ctatccctac atcatgtatg aagacacggt
     2821 taaccgtgct aaccctatcg ccgggcgcat aaatatgagc aatctctgct cagaaatttt
     2881 gcaggttaac agcgcctcag agtatgacga gaatctcgac tatgcccgca caggccatga
     2941 tatttcctgc aatttaggtt cgttgaatat tgcccacacc atggattccc ccgactttgc
     3001 ccgcacggta gagactgccg tgcgcggttt aacggcagta tcagatatga gtcatatccg
     3061 cagcgtgccg tccatcgaag ccggaaatgc cgcctcgcac gccatcggac tggggcagat
     3121 gaatttacac ggttatctgg cgcgagaagg catcgcttat ggatcgccgg aagcactgga
     3181 tttcaccaat ctctatttct ataccatcac ctggcacgca ctgcgtacct cgatgttgct
     3241 ggcgcgcgaa cgcagtgaaa ccttcgccgg gttcaaacag tcacgctatg ccaatggtga
     3301 atatttcagc caatatctgc aagggaactg gcagccgaaa acggcgaaag ttggcgaact
     3361 gtttgcccgt agcggtatta cgttacctac ccgtgagatg tgggcgcagc tgcgcgacga
     3421 cgtgatgcgc tatggcatat acaaccagaa tctccaggcg gtgccgccaa caggttccat
     3481 ctcttatatc aaccatgcca cgtcgagtat tcatccgatt gtggcgaaag tagagatacg
     3541 caaagagggc aaaacaggac gcgtttacta ccctgccccg tttatgacta acgaaaatct
     3601 ggcgctgtat caggacgctt acgaaatcgg cgcagaaaag atcatcgaca cctacgcgga
     3661 agcgactcgc catgtcgatc aggggctgtc gctgacgctg tttttccccg ataccgccac
     3721 cactcgcgat atcaacaaag cgcagattta cgcctggcgc aagggtatca aaacgctcta
     3781 ttacatccgc ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcgtg
     3841 tgcactttaa ggaatatcta tgaaactctc tcgtatcagc gccatcaact ggaacaagat
     3901 atctgacgat aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga
     3961 aaaggtgccg ctgtcgaacg atattcctgc atggcagaca ttaagtgtcg tagaacaaca
     4021 actgacgatg cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg
     4081 cgcgccttcg ctgttgcccg atgcactcac gcctcatgaa gaagcggtat tatcgaatat
     4141 cagctttatg gaagcggttc atgcccgctc ttacagttcg attttctcga cgctgtgcca
     4201 gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa
     4261 agctcagatt attcagcaac attatcgcgg ggatgatccg ctgaaaaaga aaatcgccag
     4321 tgtgtttctt gaatcttttt tgttctattc cggtttctgg ctgccgatgt atttttccag
     4381 ccgcggaaag ctaaccaata ccgcagacct gatccgtctg attatccgcg atgaagcagt
     4441 ccacggttat tacatcggct ataaatatca gaaaaacatg gaaaagatct cccaggcaca
     4501 acgtgaagag ttgaagagtt tcgccttcga tttgttgctg gaactttatg acaacgagtt
     4561 gcaatacacc gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct
     4621 ctgttacaac gccaataagg cgttgatgaa tctgggctac gaaccgttat ttcccgcaga
     4681 aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca
     4741 cgatttcttt tccggttcag gctcttcata tgtgatggga aaagcggtcg aaacagaaga
     4801 tgaagactgg aatttctgag ggtgttattt ttcaaaaata tcactacccg cagcagggaa
     4861 ataattcccg ccaaatagct ttttatcacg caaataattt gtggtgatct acactgacac
     4921 tctgttgcat tattcgcctg aaaccacaat attcaggcgt tttttcgcta tctttgacaa
     4981 aaaatatcaa cttttctcga tttgctctca gcccttatat catgggaaat tccggcgatt
     5041 tgctcacatc aatattcatg ccacatttgc catcaggggt tgcctcagat tctcagtatg
     5101 ttagggtaga aaaaagtgac tatttccatt gggtaatgta tcgacataaa caaataacag
     5161 gaatctttct attgcatggc aattaaatta gaaattaaaa atctttataa aatatttggt
     5221 gagcatccac agcgagcttt caaatatatc gaacaaggac tttcaaaaga acaaattctg
     5281 gaaaaaactg ggctatcgct tggcgtaaaa gacgccagtc tggccattga agaaggcgag
     5341 atatttgtca tcatgggatt atccggttcg ggtaaatcca caatggtacg ccttctcaat
     5401 cgcctgattg aacccacccg cgggcaagtg ctgattgatg gtgtggatat tgccaaaata
     5461 tccgacgccg aactccgtga ggtgcgcaga aaaaagattg cgatggtctt ccagtccttt
     5521 gccttaatgc cgcatatgac cgtgctggac aatactgcat tcggtatgga attggccgga
     5581 attaatgccg aagaacgccg ggaaaaagcc cttgatgcac tgcgtcaggt cgggctggaa
     5641 aattatgccc acagctaccc ggatgaactc tctggcggga tgcgtcaacg tgtgggatta
     5701 gcccgcgcgt tagcgattaa tccggatata ttattaatgg acgaagcctt ctcagcactc
     5761 gatccattaa ttcgcaccga gatgcaggat gagctggtaa aattacaggc gaaacatcag
     5821 cgcaccattg tctttatttc ccacgatctc gatgaagcta tgcgtattgg cgaccgaatt
     5881 gccattatgc aaaatggtga agtggtacag gtcggcacac cggatgaaat tctcaataat
     5941 ccggcgaatg attatgtccg taccttcttc cgtggcgtcg atattagtca ggtattcagt
     6001 gcgaaagata ttgctcgccg gacaccgaac ggcttaatcc gtaaaacccc cggcttcggc
     6061 ccgcgttcgg cactgaaatt attgcaggat gaagatcgcg aatatggcta cgtcatcgaa
     6121 cgcggtaata agtttgtcgg cgcagtgtct atcgattcgc ttaaaaccgc gttaacgcag
     6181 cagcaaggtc ttgatgcggc gctgattgat gcgccattag cggtcgatgc gcaaacgcct
     6241 cttagcgagt tgctctctca tgtcggacag gctccctgtg cggtgcccgt ggtcgacgag
     6301 gaccaacagt atgtcggcat catttcgaaa ggaatgctgc tgcgcgcttt agatcgtgag
     6361 ggggtaaata atggctgatc aaaataatcc gtgggatacc acgccagcgg cggacagtgc
     6421 cgcacagtcc gcagacgcct ggggtacacc ggctgctgca ccgactgacg gcggtggtgc
     6481 tgactggctg accagtacgc ctgcgccaaa cgtcgagcat tttaatattc tcgatccgtt
     6541 ccataaaacg ctgatcccgc tcgacagttg ggtcactgaa gggatcgact gggtcgttac
     6601 ccatttccgt cccgtcttcc agggcgtgcg cgttccggtt gattatatcc tcaacggttt
     6661 ccagcaattg ctgctgggta tgcccgctcc ggtggcgatt atcgttttcg ctctcatcgc
     6721 ctggcaaatt tccggtgtcg gaatgggcgt ggcgacgctg gtttcgctga ttgccatcgg
     6781 cgcaatcggt gcctggtcac aggcaatggt tactctggcg ctggtgctaa ccgccctgct
     6841 gttctgtatc gtcatcggtt tgccgttggg gatctggctg gcgagaagtc cacgagcggc
     6901 gaaaattatt cgtccactgc ttgatgccat gcagaccacg ccagcgtttg tttatctggt
     6961 accaatcgtc atgctgtttg gtatcggtaa cgtgccgggc gtagtggtga cgatcatctt
     7021 tgctctgccg ccgattatcc gtctgacaat tctggggatt aaccaggttc cggcggatct
     7081 gattgaagcc tcgcgctcat tcggtgccag cccgcgccag atgctgttca aagttcagtt
     7141 accgctggcg atgccgacca ttatggcggg cgttaaccag acgctgatgc tggccctttc
     7201 tatggtggtc atcgcctcga tgattgcagt cggcgggctg ggtcagatgg tacttcgcgg
     7261 tatcggtcgt ctggatatgg ggcttgccac cgttggcggc gtcgggattg tgatcctcgc
     7321 cattatcctc gaccgcctga cgcaggccgt tgggcgcgac tcacgcagtc gcggcaaccg
     7381 tcgctggtac accactggcc ctgttggttt gctgacccgc ccattcatta agtaactctg
     7441 cacttgcccg gtgacgccgg gcattatcac cctgccaaaa aaaggaataa caatgcgaca
     7501 tagcgtactt tttgcgacag cgtttgccac gcttatctct acacaaactt ttgctgccga
     7561 tctgccgggc aaaggcatta ctgttaatcc agttcagagc accatcactg aagaaacctt
     7621 ccagacgctg ctggtcagtc gtgcactgga gaaattaggt tataccgtca ataaacccag
     7681 cgaagtggat tacaacgttg gctacacctc gcttgcttcc ggcgatgcaa ccttcaccgc
     7741 cgtgaactgg acgccgctgc atgacaacat gtacgaagct gccggtggcg ataagaaatt
     7801 ttatcgtgaa ggagtatttg ttaacggcgc ggcacagggt tacctgatcg ataagaaaac
     7861 tgccgaccag tacaaaatca ccaacatcgc gcaactgaaa gatccgaaga tcgccaaact
     7921 gttcgatacc aacggcgacg gtaaagcgga tttgactggc tgtaaccctg gctggggctg
     7981 cgaaggtgcg atcaaccacc agcttgccgc gtatggactg accaataccg tgacgcataa
     8041 tcaggggaac tacgcagcga tgatggctga caccatcagt cgttacaaag agggtaagcc
     8101 ggtgttttac tacacctgga cgccgtactg ggtgagtaac gaactgaaac cggggaaaga
     8161 tgtggtctgg ttgcaggtgc cgttctccgc actgccgggc gataaaaatg ccgataccaa
     8221 actgccgaat ggcgcgaatt acggcttccc ggtcagtacc atgcatatcg ttgccaacaa
     8281 agcctgggcc gagaaaaacc cggcagcagc gaaactgttt gccattatgc agttaccagt
     8341 ggcagatatt aacgtccaga acgccattat gcatgacggc aaagcctcag aaggcgatat
     8401 tcagggccac gttgatggct ggatcaaagc ccaccagcag cagttcgatg gctgggtgaa
     8461 tgaggcgctg gcagcgcaga agtaaatatt gataaccgcg cagataatgc tcatgtgagt
     8521 aaaacaattg cagcgatggt tttaatccgg ctgaaaatcg atgagacgtt ttctgaaggc
     8581 cggggcgacg cgcagggatg cgcggcgagg gcgcgtctta cagggatgtt acctcgcgcc
     8641 cgtacccgat agccgtaaga aataagtcga atgtaccgcg tagcggcgat tttgtgccaa
     8701 aagtttgggt cgccagggtg gtggcgactg agccaccctg gcacgttcac cagcaaaata
     8761 gtgacaaagt agctataatc tggaagtgaa cgtaacattt gccttagtgg catatttcac
     8821 cttcaaatac cctctcccca ttacccgcac attttaacaa tctatctttc gccccatatt
     8881 catcaacatc cgctattatt gatttccagc ttaatcatca cctgatgaac aaaaataatg
     8941 actaaaccta atcatgagct tagcccggca ctgatcgtac tgatgtctat cgccaccggt
     9001 ctggcggtcg ccagcaacta ttacgcccag ccattgctcg acaccattgc gcgtaacttt
     9061 tccctttccg ccagttcggc gggctttatt gttaccgccg cgcagttggg ctatgccgca
     9121 ggtctgctgt ttcttgttcc cctcggtgat atgtttgaac gccgccgcct gattgtctcg
     9181 atgaccttac tggcagcggg cggtatgtta atcactgcca gcagtcagtc actggcgatg
     9241 atgatcctcg gtacggcatt aaccggttta ttctcagtcg tggcacaaat tctggttccg
     9301 ctggcagcga cgctggcttc accggataaa cgcggcaaag tggtcggcac cattatgagc
     9361 gggctgctgt tggggatctt gctggcacga accgttgccg ggttgctggc aaacctcggc
     9421 ggctggcgca ccgtcttttg ggttgcctcg gtgttaatgg cactgatggc gctggcatta
     9481 tggcgtggtc tgccacaaat gaaatcagaa acccacctca actacccaca gttgttgggt
     9541 tccgttttca gcatgtttat cagcgataag atcctgcgca cccgtgcgtt gctgggctgc
     9601 ctgacctttg ccaacttcag cattctctgg acctcaatgg cctttttgct tgccgctcca
     9661 ccttttaact acagcgatgg tgtaattggt ctgtttggac ttgcgggagc tgccggagcg
     9721 ttgggtgctc gtccggcggg cggttttgcc gataagggta aatcgcacca caccactact
     9781 ttcggtctgc tgctgctatt actttcatgg ctggcgatct ggtttggcca cacttccgta
     9841 ctggccttga ttatcggcat cctggtgctg gatctcaccg tgcagggcgt gcatatcact
     9901 aaccagacgg taatttatcg aatacaccct gatgcgcgta atcgcctgac cgcaggttac
     9961 atgaccagct actttattgg cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa
    10021 catggcggtt gggctggcgt ttgtctggct ggcgcgacga ttgccctggt taacttactg
    10081 gtctggtggc gaggttttca tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt
    10141 cgtggcttta cacttataag ggtgttaaga agcccatcag tctgataagg ttaagatatt
    10201 cattcagtct atttataata ttaacaatcg ttaagcgtac actctatgga aagccctact
    10261 ccacagcctg ctcctggttc ggcgaccttc atggaaggat gcaaagacag tttaccgatt
    10321 gttattagtt atattccggt ggcctttgcg ttcggtctga atgcgacccg tctgggattc
    10381 tctcctctcg aaagcgtttt tttctcctgc atcatttatg caggcgcgag ccagttcgtc
    10441 attaccgcga tgctggcagc cgggagtagt ttgtgggttg ctgcactgac cgtcatggca
    10501 atggatgttc gccatgtttt gtatggcccg tcactgcgta gccgtattat tcagcgtctg
    10561 caaaaatcga aaaccgccct gtgggcattt ggcctgacgg atgaggtttt tgccgccgca
    10621 accgcaaaac tggtacgcaa taatcgccgc tggagcgaga actggatgat cggcattgcc
    10681 ttcagttcat ggtcatcgtg ggtatttggt acggtaatag gggcattctc cggcagcggc
    10741 ttgctgcaag gatatcccgc cgttgaagca gcattaggtt ttatgcttcc ggcactcttt
    10801 atgagtttcc tgctcgcctc tttccagcgc aaacaatctc tttgcgttac cgcagcgtta
    10861 gttggtgccc ttgcaggcgt aacgctattt tctattcccg tcgccattct ggcaggcatt
    10921 gtctgtggct gcctcactgc gttaatccag gcattctggc aaggagcgcc cgatgagcta
    10981 tgaggttctg ctgcttgggt tactggttgg cgcggcgaat tattgcttcc gctatttgcc
    11041 gctgcgcctg cgtgtgggta atgcccgccc aaccaaacgt ggcgcgatag gcatattgct
    11101 cgacaccatt ggcatcgcct cgatatgcgc tctgctggtt gtctctaccg caccagaagt
    11161 gatgcacgat acacgccgtt tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag
    11221 tttctataaa acacgcagca ttatcatccc aacactgctt agtgcgctgg cctacgggct
    11281 cgcctggaaa gtgatggcga ttatataacc cacaagaatc atttttctaa aacaatacat
    11341 ttactttatt tgtcactgtc gttactatat cggctgaaat taatgaggtc atacccaaat
    11401 ggatagttcg tttacgccca ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga
    11461 ttttccttat caggagatcc ttctgacccg tctttgcatg cacatgcaaa gcaagctgct
    11521 ggagaaccgc aataaaatgc tgaaggctca ggggattaac gagacgttgt ttatggcgtt
    11581 gattacgctg gagtctcagg aaaaccacag tattcagcct tctgaattga gttgtgctct
    11641 tggatcatcc cgtaccaacg cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat
    11701 tgaacgtcgt gaaagcgata acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg
    11761 tcacgagttt ttgcgcgagg ttttaccacc gcagcataac tgcctgcatc aactctggtc
    11821 cgcgctcagc acaacagaaa aagatcagct cgagcaaatc acccgcaaat tgctctcccg
    11881 tctcgaccag atggaacaag acggtgtggt tctcgaagcg atgagctaac gcgtcatctc
    11941 gctcaaaaat ccagatttat aaaagaaaaa atgactggcc agcatcgcaa catgctggcc
    12001 tttttggcaa gcaggtcggc tcagccgatg agttaagaag atcgtggaga acaatatgag
    12061 cgcaaatgcg gagactcaaa ccccgcagca accggtaaag aagagcggca aacgtaagcg
    12121 tctgctcctc cttctcacct tgctctttat aattattgcc gtagcgatag ggatttattg
    12181 gtttttggta ctgcgtcatt tcgaagaaac cgatgacgca tacgtggcag ggaatcagat
    12241 tcaaattatg tctcaggtgt ctggcagcgt gacgaaagtc tgggccgata acaccgactt
    12301 tgtaaaagaa ggcgatgtgc tggtcaccct cgacccgaca gatgctcgcc aggcgtttga
    12361 aaaagccaaa actgcactgg cttccagcgt tcgccaaacc caccagctga tgattaacag
    12421 caaacagttg caggcgaata ttgaggtgca aaaaatcgca ctcgcgaaag cacaaagcga
    12481 ctacaaccgc cgtgtgccgc tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca
    12541 cgcccgcgac gccgttacca gtgcccaggc acaactggac gtcgcgattc aacaatacaa
    12601 tgccaatcag gcgatgattc tggggactaa actggaagat cagcctgccg tgcaacaggc
    12661 tgccaccgaa gtacgtaacg cctggctggc gctggagcgt actcgtattg tcagtccgat
    12721 gaccggttat gtctcccgcc gcgcggtaca gcctggggca caaattagcc caacgacgcc
    12781 gctgatggcg gtcgttccag ccaccaatat gtgggtggat gccaacttta aagagacgca
    12841 gattgccaat atgcgcatcg gtcagccggt caccatcacc accgatattt acggcgatga
    12901 tgtgaaatac accggtaaag tggttggtct ggatatgggg acaggtagcg cgttctcact
    12961 gcttccggcg caaaatgcga ctggtaactg gatcaaagtc gttcagcgtc tgcctgttcg
    13021 tattgaactg gaccagaaac agctcgagca atacccgctg cgtatcggtt tgtccacgct
    13081 ggtgagcgtc aataccacta accgtgacgg tcaggtactg gcaaataaag tacgttccac
    13141 accggtagcg gtaagcaccg cgcgtgaaat cagcctggca cctgtcaata aactgatcga
    13201 cgatatcgta aaagctaacg ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac
    13261 cgctggaagg cgcgcaactg gtcattatga cgattgcgct gtcactggcg acattcatgc
    13321 aggtgctgga ctccaccatt gctaacgtgg cgatccccac tatcgccggg aatctgggct
    13381 catcgctcag ccagggaacg tgggtaatca cttctttcgg ggtggcgaat gccatctcga
    13441 tcccgcttac cggctggctg gcaaagcgcg tcggggaagt gaaactgttc ctctggtcaa
    13501 ccatcgcctt tgctattgcg tcgtgggcgt gtggtgtctc cagcagcctg aatatgctga
    13561 tcttcttccg cgtgattcag gggattgtcg ccggaccgtt gatcccgctt tcgcaaagtc
    13621 tattgctgaa taactacccg ccagccaaac gctcgatcgc gctggcgttg tggtcaatga
    13681 cggtgattgt cgcgccaatt tgcggcccga tcctcggcgg ttatatcagc gataattacc
    13741 actggggctg gatattcttc atcaacgtgc caattggcgt ggcggtggtg ttgatgacac
    13801 tgcaaaccct gcgcggacgc gaaacccgca ccgaacggcg gcggattgat gccgtggggc
    13861 tggcactgct ggttattggt ataggcagcc tgcagattat gctcgaccgc ggtaaagagc
    13921 tggactggtt ctcatcacag gaaattatca tccttaccgt ggtggcagtg gtggctatct
    13981 gcttcctgat tgtctgggag ctgaccgacg ataacccgat agtcgatctg tcgttgttta
    14041 agtcgcgcaa cttcaccatc ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg
    14101 gcgcgattgt tctgctgccg cagttgttgc aggaggtcta cggttacacg gcaacgtggg
    14161 caggtctagc ctctgcgccg gtaggaatta ttccggtgat cctgtcgccg attatcggcc
    14221 gcttcgcgca taaactggat atgcggcggc tggtaacctt cagctttatt atgtatgccg
    14281 tctgcttcta ctggcgtgcc tatacctttg aaccaggtat ggattttggc gcgtcggcct
    14341 ggccgcagtt tatccagggt tttgcggtgg cctgcttctt tatgccgctg accaccatta
    14401 cgctgtctgg tttgccaccg gaacgactgg cggcagcatc gagcctctcc aactttacgc
    14461 gaacgctggc ggggtctatc ggcacgtcga taaccacaac catgtggacc aaccgcgagt
    14521 cgatgcacca tgcgcagttg actgagtcgg taaacccgtt caacccgaat gcccaggcga
    14581 tgtacagtca actggaaggg cttgggatga cgcaacagca ggcatcaggc tggattgccc
    14641 agcagatcac caatcagggg ctgattattt ccgccaatga gatcttctgg atgtcagccg
    14701 ggatattcct cgtcttgctg gggctggtgt ggtttgctaa accgccattt ggcgcaggtg
    14761 gcggcggagg tggtgcgcac taagtacaac taagccagtt catttgaact ggcttttttc
    14821 aattaattgt gaagatagtt tactgactag atgtgcagtt cctgcaactt ctctttcggc
    14881 agcgccagtt cttcgttgct gttgatgcgt acgtcacgtt ccagaatgct acgcgcaata
    14941 tcctgtgctt cctgcaacga gtgcatctgg taagtgccac actggtagac gttcagttcc
    15001 gggatctgat tctgatcctg cactttcagc acgtcttcca ttgccgcttt ccaggcatca
    15061 gcgacacgct gctcatctgg cgtaccaatc agactcatat aaaaaccggt gcggcagccc
    15121 attggcgaga tatcgataat ctctacacca ttaccgttaa gatggttacg cataaaacca
    15181 gcaaacaggt gctccagggt atggatccct ctttctggca tcacttcttt gttcggcacg
    15241 cagaagcgca gatcgaacac ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc
    15301 acccgaactg caggcgcttc catccgggta tgatcgactg tgaagctatc taacaacggc
    15361 atttagccac ctccggtaat ttttttaaaa attttctgaa ctctttcttc ccaggcgagt
    15421 ctgagtatat gaaagacgcg catttgttat catcatccct gaattcagag atgaaatttt
    15481 ggccactcac gagtggcctt tttcttttct gtcaggcgtg tttttccagc cacaccgcaa
    15541 acggttcggt atcagcggct tccatttcct gctgacggcg ttcagacgcc tcgcgctcgg
    15601 ctacaaaatc ctcttcgcgc agaatttcca acggctcttc acgcagcaga ttacggtacg
    15661 cttcagcaaa cgctttgcct gttccgccaa taccagtatc aatcatagac cttaagatac
    15721 gggcagagaa agtcagatcg ggattatcga agcaggcgac cagttcatca cacactttct
    15781 gatacgcttc gccgccgtta atgctatcca gcgtttgcgc aacgcgtttc agatcgcgga
    15841 acagatcttt acccacctgc ggtaacggga actgcgcggt ttcgcagccg atacccagcg
    15901 tcaggcccgg tttgcgacct tcgaggatca cccggttcca gttaacgcgt gtacaggcaa
    15961 gttcgcgact gctcatttcc ggtgcatcag ccagcgcaca ccagaccata aacaggtcga
    16021 ggaatcgcac ctgctgttca tctacaccaa tcggcgagaa tgggttgatg tccagcgaac
    16081 gcacttcaat atattcaatg ccgccacgta acagcgcatc agaaggcgac tcaccactgc
    16141 gggtaacgcg ttttggacga atcggcgcgt acagttcgtt ttcaatctgc agcacattgc
    16201 tgttgatttg cagccgctta ccgtctttct caataccaat cttcgcgtac tcttccgatg
    16261 gcgttttgat tgcctgttta aggcccgcta cgtactcgta aagatcgttg aaggtaatac
    16321 caagattgct ttgcgatttg ttggtatagc caagatcgct caaacgaaga gaggtcgcat
    16381 acggcaggta atacataccg cactcggttt tctcaaacgg cagcgacgtt ggttttcctt
    16441 gcaggaaaga agaacaaatc gccggagacg cgccaaacag ataaggaatg acccaaccga
    16501 aacgatagta attgcggata acgcggaaat agcccgcaga aattttctct ttggcatcag
    16561 cgcccgcgat atcaccacac ttcgcttgcc agaatgccat tggcaaagag aaattgtagt
    16621 gcacgccgga aatggtttgc atcagcgcgc cgtagcgatt tttcagccct tcacggtaca
    16681 gcgttttaaa gcgtccggtg ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt
    16741 ctgcgatgta gcatggcata cttaacggcc acatccgctc atcgcccata ttgcgcgccg
    16801 tataacgatg cagatcgcgc ataaaggtca gcatatgttc aatatcacca tccactggtg
    16861 taatgaattc cagtaatgct tccgcaaaat cggtagtaat ccatttgtgc gtcagtgcgg
    16921 aacctaatgc ttcaggatga cctgttgttg ccagtctgcc atcagcatta acacgcaaag
    16981 tttcgcgctc cagcccacgc tgtatcccct ttaacgcctg aggatgtttt tccagccagg
    17041 ccagcgcctg tgatacgtcc gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat
    17101 tagcataact gtaatggtga ccatatgtgc aggcctacaa ttagtgccac cacatcatgc
    17161 cctgaacggt cgctgctgca actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa
    17221 agattaccgg cccccaggag atgcgcatcc atcccgctaa cagacacagt aaatcgccaa
    17281 ccacgggcat ccagcttaat aatagcgtga ctgcaccata gcgtttcagc cagccggtag
    17341 ctttctcttg ccagcgcgat gttttacgca atggaaagaa acgcccaagg ataacgttag
    17401 ttaaccctcc aaggctatta cccattgttg ctgttaaaac taaaacccag ggatgactga
    17461 tcccggaaag caacattgcc accagcacga cttcggagtt gccgggtaat agtgtagcgc
    17521 tgagaaaact actggcaaac aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac
    17581 atccacagcg tccatgcctg ctgcacgggc cgccagaata ccgaaatcgg catcttcaaa
    17641 gaccacacac tgtgtcggtt gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg
    17701 cgcgggttta tggtgtttga cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa
    17761 tcccaggtgc gccagcaatg cctcagcgat ggcgctttca ctccccgttc ctacagccat
    17821 tgggcgacga ccatgccagc ttttcaccac ttcaacaaga ggaagcggct cgacactatc
    17881 cagcagcata cttcttactg cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc
    17941 ggcctgattc agctcaataa tcgcctgagc aatacgccag gtgggcgagc cattaagcgc
    18001 aatcatcgcc tgaacatcgt actgaagacc gtagtgccct aatacttcgc gccacgcttt
    18061 acggtgcgta ggctcggtat ccaggatggt gccatccata tcaaaaatta aacctgcata
    18121 acgctcgtac atggtcttct cgcaagtcga aaaatatgac gctactttag cgtaattgct
    18181 tgattttgtc gctgatggcg aaggagaaga aaattcagga aatggataaa gtagttattt
    18241 agaaatgaga tatttttgaa ggaaatttgt gggagaagat ggtgcctgcg gttagtaccg
    18301 ctacctcttc gataccttca ttgctgaaga ttacggagaa tatggtgcat ccgggaggat
    18361 tcgaacctcc gaccgctcgg ttcgtagccg agtactctat ccagctgagc tacggatgca
    18421 tcgggaaaac ttattttact gctgatattg ataccgctac taaagccata tcaaataaga
    18481 aaatccatct taaccatcat cactgatgat tacggagaat atggtgcatc cgggaggatt
    18541 cgaacctccg accgctcggt tcgtagccga gtactctatc cagctgagct acggatgcat
    18601 cgg
//
LOCUS       DAPOZO010000091        18513 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPOZO010000091 DAPOZO010000000
VERSION     DAPOZO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.091"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            161..700
                     /locus_tag="ROI21_003576"
     CDS             161..700
                     /locus_tag="ROI21_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HDX5112453.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARIHLKRTILALPES
                     NVIHAGYGSYAVIEVDLNGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(1110..2243)
                     /gene="thiH"
                     /locus_tag="ROI21_003577"
     CDS             complement(1110..2243)
                     /gene="thiH"
                     /locus_tag="ROI21_003577"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDX5112454.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDGRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(2240..3010)
                     /gene="thiG"
                     /locus_tag="ROI21_003578"
     CDS             complement(2240..3010)
                     /gene="thiG"
                     /locus_tag="ROI21_003578"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDX5112455.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAIADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(3012..3212)
                     /gene="thiS"
                     /locus_tag="ROI21_003579"
     CDS             complement(3012..3212)
                     /gene="thiS"
                     /locus_tag="ROI21_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDX5112456.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(3196..3951)
                     /gene="thiF"
                     /locus_tag="ROI21_003580"
     CDS             complement(3196..3951)
                     /gene="thiF"
                     /locus_tag="ROI21_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDX5112457.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPKRNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(3944..4579)
                     /gene="thiE"
                     /locus_tag="ROI21_003581"
     CDS             complement(3944..4579)
                     /gene="thiE"
                     /locus_tag="ROI21_003581"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDX5112458.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLPRAPAVMATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(4579..6474)
                     /gene="thiC"
                     /locus_tag="ROI21_003582"
     CDS             complement(4579..6474)
                     /gene="thiC"
                     /locus_tag="ROI21_003582"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDX5112459.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGLLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSVQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(6555..6655)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6707..7183)
                     /gene="rsd"
                     /locus_tag="ROI21_003583"
     CDS             complement(6707..7183)
                     /gene="rsd"
                     /locus_tag="ROI21_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDX5112460.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            7278..8051
                     /gene="nudC"
                     /locus_tag="ROI21_003584"
     CDS             7278..8051
                     /gene="nudC"
                     /locus_tag="ROI21_003584"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDX5112461.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGVLPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            8091..9155
                     /gene="hemE"
                     /locus_tag="ROI21_003585"
     CDS             8091..9155
                     /gene="hemE"
                     /locus_tag="ROI21_003585"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDX5112462.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            9312..9983
                     /gene="nfi"
                     /locus_tag="ROI21_003586"
     CDS             9312..9983
                     /gene="nfi"
                     /locus_tag="ROI21_003586"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDX5112463.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            10026..10616
                     /locus_tag="ROI21_003587"
     CDS             10026..10616
                     /locus_tag="ROI21_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDX5112464.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            10803..11075
                     /gene="hupA"
                     /locus_tag="ROI21_003588"
     CDS             10803..11075
                     /gene="hupA"
                     /locus_tag="ROI21_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDX5112465.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            11088..11783
                     /locus_tag="ROI21_003589"
     CDS             11088..11783
                     /locus_tag="ROI21_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDX5112466.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(11785..12210)
                     /gene="zraP"
                     /locus_tag="ROI21_003590"
     CDS             complement(11785..12210)
                     /gene="zraP"
                     /locus_tag="ROI21_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDX5112467.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     ETAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCSGGGHMGMGHW"
     gene            12448..13845
                     /gene="zraS"
                     /locus_tag="ROI21_003591"
     CDS             12448..13845
                     /gene="zraS"
                     /locus_tag="ROI21_003591"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDX5112468.1"
                     /translation="MRFMQRSKDTLAKWLSAILPVVIVGLVGLFAVTVIRDYGRASEA
                     DRQALLEKGNVLIRALESGSRVGMGMRMHHVQQQALLEEMAGQPGVLWFAVTDAQGII
                     ILHSNPEMVGRALYSPDEMQQLKPEENSRWRLLEDAEKTPALEVYRLFQPMSLPWRHG
                     MHNMPRCNGKAVPQAEAQQAIFIAVDASDLVATQSGEKRNTLIILFALATVLLASVLS
                     FFWYRRYLRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPA
                     GGEAHQLAQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANS
                     REIQLRFTANDMLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKIS
                     VTDSGKGIAADQLEAIFTPYFTTKADGTGLGLAVVHNIVEQHGGTIQVASQEGEGATF
                     TLWLPVNITRKDPQG"
     gene            13842..15167
                     /gene="zraR"
                     /locus_tag="ROI21_003592"
     CDS             13842..15167
                     /gene="zraR"
                     /locus_tag="ROI21_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDX5112469.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGQQALAQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHMHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LAQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(15164..16453)
                     /gene="purD"
                     /locus_tag="ROI21_003593"
     CDS             complement(15164..16453)
                     /gene="purD"
                     /locus_tag="ROI21_003593"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDX5112470.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPTLQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMKRIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRIKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVSDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(16465..18054)
                     /gene="purH"
                     /locus_tag="ROI21_003594"
     CDS             complement(16465..18054)
                     /gene="purH"
                     /locus_tag="ROI21_003594"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDX5112471.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEI
                     DANEGSLTLETRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         4209 a         4938 c         5067 g         4299 t
ORIGIN      
        1 ggtagtttag ggaaagagca tttgtcagaa tatttaatga atttctggat actcataatc
       61 aatgtagaga tgactaatat cctgaaactg actgaactaa ttgagtcaag ctcggcaaga
      121 attcgatact attcctgtgt gactttctta aggaacgata atgaaacagg aagtggagaa
      181 gtggcgacct tttggacatc cggatggtga tattcgtgat ttatcatttc ttgatgctca
      241 tcaggctgtc tacgttcagc atcatgaggg caaagagcct ttagagtatc gcttctgggt
      301 tacctactct cttcactgct tcacaaaaga ttatgaacat cagactaacg aagaaaaaca
      361 atcgttaatg taccacgcgc ctaaagaatc tcgtcccttc tgccagcacc gttataactt
      421 agcgcgcata cacttaaaaa gaaccatttt ggcgctgcca gaaagcaacg ttattcatgc
      481 tgggtatggt agctatgccg tgattgaagt ggatttaaac ggaggagaca aggcatttta
      541 ctttgttgcg ttcagggctt tcagggaaaa gaaaaaactt cgtttgcatg taactagcgc
      601 ttatcccatt tctgaaaaac agaaaggtaa atcagtgaaa tttttcacca ttgcctacaa
      661 cttattgaga aataagcagc ttcctcagcc ctcaaaataa caaaacccac cttaaggtgg
      721 gtttcgccag agaattatct ctggtattca gaacgccatt accggacttt gccttgacct
      781 tgcgataatc gcaggttgcg ggatgtctga atttcttcag tctgctgcat cctggaagat
      841 gagaacatgt gttcttattt tcgtctctat catagttgag tatttactct cttacaatca
      901 gatctctttc attgctcaac aggcgatggc ttcagacttt gcattacgga atttttaaga
      961 aaggcagggc gaaacgagga agaagctttt cgccctggac aacattcctg ctgacggcac
     1021 taccataaaa aaacacaacg ctttcaacaa ccttgcgttt ttcatcttcc gtagcggctc
     1081 ggaataaatt tacgttctta cattctgtct catggccttt gcgaggcgcg tcccagatag
     1141 ctgtcccagt ctttccatac cggctgcaaa ccctgagcag ttaacgcggc agcaaccgct
     1201 tccggtctgc gaccgtcgtg cggtgagaac tgttccagct cggggtgatt gtcggcatag
     1261 ccacctggct gcgttttcga aaaagcgctg acgttattaa ttgccagcgg aatcacgcga
     1321 tcgcgaaacc acggtgattc ccgcgtggag agtgacagtt caatctccgg tgcaagcagc
     1381 cggaaggcgc agatggtttg cactaactgg cgttcatcca taatcgacgc aggctcaatg
     1441 ccgccagtac atggacgcag gcgtgggaag gagacagagt agcggctttg ccagtaatgc
     1501 tgttgcagcc atagcaaatg ttctgcaacc atatagcagt caacgcgcca gctgtcggaa
     1561 aggccaatta gcgcgccgag gcctatctta tcaatccccg cacggcccag ccgatccggc
     1621 gtttccagcc gccagaagaa gtcctgtttt ttgcctttca gatggtggcg ggcataagtc
     1681 gcctcgtgat atgtctcctg ataaaccatc acgccatcca gaccaagttg ctttaactcg
     1741 gcgtattccg tctccgccag cggctgtact tccatctgta gtgaagagaa ctgttcacgc
     1801 agggcaggga gatgacgacg aaagtaatcc atccccactt tcgcctgatg ttcaccagtg
     1861 actaaaagca gatgttcaaa gcccatctcc cgtatagcgg cgctttccct ggcaatatcc
     1921 gcttcatcca gcgttttgcg cttgatgcgg ttgctcatag agaatccgca gtacgtgcag
     1981 tcgttagcgc aaagattgga aagataaagc gggacgtaga agctgacgac gttgccaaat
     2041 cgctgacggg tcagacgctg cgcccgttgg gccagtggtt ccagatagcc actggcggct
     2101 ggcgataaca gcgccatcat atcgtcgcgg gtgagttgcg aggcatttag cgcccgctct
     2161 acgtcagcag ccgttttgcc gttgatacgc aggcggatgt cgtcccagtc cagttgtcgc
     2221 cagcgatcgc tgaaggtttt catgccgatg cctccagaaa tccggtcagc gggctggtgg
     2281 catgagcaaa atgactgcgg ctacccggtc cggactgacg tgccagcagg cctgcttcta
     2341 ctgccagacg aaatgccttc gccatgttga cgggatcgtc cgcgatggca atcgccgtat
     2401 tcactaacac cgcgtcggcc cccatttcca gcgcctgcgc ggcatggctg ggaacgccga
     2461 tgccagcgtc gacaaccacc ggaaccgtgg cttgctggat aataatctcc agcatggcac
     2521 gggtttccag tccctgattc gagccaatcg gcgcgccgag cggcatcacc gctgcacagc
     2581 cgacttcttc cagacgttta cacaataccg gatcggcccc gcagtaaggc agcacgacaa
     2641 atccctgttg taccagcgtt tcggcggctt tcagggtttc gatgggatcg ggcaacagcc
     2701 agcgggcgtc agggtgaatc tctaatttta accagtttgt gcctaacgct tcacgtgcca
     2761 gatgggcggc gaaaatggct tcttccgctg ttttcgcccc ggatgtattc ggtagcaggg
     2821 tcacacccgc cgcgataagc ggttcgagga tagcgtcgtt gtgctggcgc aagtcgacac
     2881 gtttcatcgc cagcgtcacc agttggctgc cggaagcgcg aatagactcc accatcagtt
     2941 gtgatgaggc gaatttcccg gtgccggtaa acagatgtga atcaaacgtt ttgtccgcaa
     3001 tacgtaacat ctcaaccccc tgcaataacc tgaaaaagca ggatctggtc gccatcctgc
     3061 acgatatgtt gcgcccactg ctcacgcggg acgatttgct gattaatcgc cagagccgcg
     3121 cccgcttgtc gttggtcgag ttgctccagt agttcgtgaa ccgtttgccc ggcggcgcac
     3181 tgcatcggtt gatcgttaaa caggatctgc attgcttcct ccgcataccg ggcaaccact
     3241 ggcgcggcgc aacgccaggc tgcgccactg gctcgactta ccgtcgaaca gtcggagttc
     3301 tcccgcaggc gtctctatac cgctcaataa cttaatggct tccagtgcct gcaaagtgcc
     3361 cataaccccg accaccgggc caaccacgcc cgctgtgcgg cagttgcgtt taggctcctg
     3421 gttatctggc cacaggcagc ggtaacaccc ctgctcccag ggcggcgtca gtaccatcaa
     3481 ctgaccgcca aatccgaccg cgctggcggt gataagcggc gtgttgagtg ccacgcaggc
     3541 ggcattaatc tcctggcgag tcgccatatt gtcggtacag tcgagcacca catcggcccg
     3601 tgctaccgca tcttttaacg cctcacccgt taaccgttgt tgtagtgccg tcagttgaat
     3661 gtcgggattc aactgtgtca gtcgctgttg gctgacctgc gatttcgggc gatcgatatc
     3721 ttcagtggta aagaggattt gtcgttgcag attgcttaaa tgcacatcgt cgtcatctgc
     3781 cagtaccagc gtcccgacgc cagcgcccgc caggtacagc gcagcaggtg tccccagccc
     3841 gccaagacca ataatcagca cctggctgtc gagcagtttt tgctgcccgt caagagcgat
     3901 atcgtcgagc aggatttggc ggctataacg cataaagtca cggtcattca tcgccaactc
     3961 ctgcaatttc cagcaactgt gccgttgcca aacgccagtc tgcggcttga gtaatggcgc
     4021 tgacaacggc gatactgccg acccccgtcg ccattaccgc tggcgcgcgc ggcagactga
     4081 taccgccaat cgccacggtg ggataatccg ccaggcgctc aacatgccgt gccagctgtt
     4141 ccagcccctg cggtgcggaa ggcatctgtt tggtttgcgt cgggaacaca tgtcccagcg
     4201 cgatataaga aggacgtgct gccagcgcga cgtcgatttc catatcatca tgtgtcgaaa
     4261 cgcccagccg cagaccagcc gcgcggatgg cgctgagatc ggtggcttgc aaatcttcct
     4321 gccccagatg gacgccatat gcctgatgct tgatcgccag ccgccagtaa tcgttgataa
     4381 acaatcgcgc gttatagcgg cggcccagcg caattgccgc cacgacatcg gcttccacct
     4441 cttcatcgcg ccgatctttg atgcgtagct ggagagtacg cacgcctgca tccaacagac
     4501 gttcgatcca ctgtacgctg tccaccaccg ggtacagtcc taaacgaaaa ggtacaggag
     4561 gaaaatcagg ctgatacatc acgcttcctc cttacgcagg tagatttctc cgcctctggc
     4621 gcggaagttc tccgacatat ccgccattcc cacttcaatg gtttgcgcgg cggcgtaatc
     4681 acgcacttcc tggctaattt tcatcgagca gaatttcggc ccacacatgg agcaaaaatg
     4741 ggcgacttta ccggactctt gcggcagggt ttcatcgtga taagcgcggg cagtaaacgg
     4801 gtcgagggcc agattaaact ggtcttccca gcgaaattcg aagcgggctt tcgacatggc
     4861 gttatcgcga atttgcgcgc ccggatgccc tttcgccagg tcagcggcgt gggcggcaat
     4921 cttataggtg ataagcccct gcttaacatc ttctttatta ggcagaccca gatgctcttt
     4981 tggcgttacg taacagagca tcgcgcagcc aaaccagcca atcatcgccg caccaatccc
     5041 cgacgtgaag tggtcatagc ccggcgcaat atcggtagtt agcggcccca gagtgtaaaa
     5101 cggcgcttcg tggcagtgct ctaactcctc ggtcatattg cggcggatca tctgcatcgg
     5161 cacgtgacct gggccttcaa tcatcacctg tacgtcatat tcccaggcga ttttggtcag
     5221 ttcacccagc gtatgcagct cggcaaactg cgcttcatcg ttggcgtcct gaacagaacc
     5281 ggggcgcaga ccgtcgccca gcgacagcga aacgtcataa gcggcacaga tttcacaaat
     5341 ttcgcggaag tgttgataga ggaaattttc ctgatgatgg gagaggcacc atttcgccat
     5401 aatcgaaccg ccgcgagaga cgataccagt caggcgtttc gcggtcatcg gtacatagcg
     5461 cagcagtacg cccgcatgga tagtgaagta atccacacct tgctcggcct gttccagcag
     5521 cgtgtcgcgg aacgcttccc aggtaagatc ttcggcgatc ccgttaacct tctccagcgc
     5581 ctggtagatc ggcactgtac caatcggcac tgggctgtta cgcaaaatcc actcgcgggt
     5641 ttcgtgaata tagcgaccgg tggagagatc catcaccgta tccgctcccc agcgcgtgga
     5701 ccataccagc ttttccactt cttcttcgat ggaagaggtg accgccgagt tgccgatatt
     5761 ggcgttaact tttaccagga aattgcgacc aataatcatc ggctccgatt ccggatgatt
     5821 aatgttggcc gggataatcg cacgtccggc agcaacttca tcacggacaa attccgcggt
     5881 gatattttcc ggcagacgtg cgccaaagct cattcccgga tgctggtggc gcaaaacttc
     5941 gctacgtatg cgctcgcgac ccatattctc gcggatggcg atgaattcca tttccggtgt
     6001 gatgatgccc tggcgggcgt agtgcagttg ggtgacgcgg cgtcctgctt tggcgcgttt
     6061 tggcgttaat aagccgctaa aacgtagctc gtcgaggcca tcatctgcca gtcgcgcttt
     6121 agtgtaatcg gaactgcgca cggtaagttc ttcggtatcg ccgcgcgcat cgatccacgg
     6181 ctggcgtagt tttgccagtc cttgctgcac gttaatggcg atctgcggat caccatacgg
     6241 gccggaggtg tcgtagactg gaatcgcttc gttttcttca aactgcggct gttctttgct
     6301 accgccaatt agcgtcgggc taagctggat ctcacgcatc ggcacgcgca cgccgggttg
     6361 tgtgccagtg atataaatgc gttttgagtt gggaaaggcg gtgccttcca gggtgtcgat
     6421 aaaatgttgg gcctgggcgc gttgttcgcg gcgggtcagt tttgttgcag acatagctca
     6481 ttccaaaaag ttaaggacgt ggcttgtcag acgacggatg aagcaagaga cgatcgccgc
     6541 aaaggcgatg cgatagcaga ttaactcttg ttcccttcgc aggtattagc ctgatcaggt
     6601 tccgcggatc ccgaataaac ggtctcagcc agttaaggca ctccgacaag aaattagccc
     6661 cacaaacggg gcgttgaatg taaattacgc gttaacagcg cagaactcaa gcaggatgtt
     6721 tgacgcgggc ggcgtcaagt accagcagaa tcaacttatc ttccagcacg aagcgtgctt
     6781 ccagcgcctc gccgatgtca gataaaacct gttgaaactc aaggtaatta tcatgatcga
     6841 tggcggtttc caggctggaa tcgtagtaat ccataatctg ttgggtattg gcttcgagtt
     6901 gcggccaaat cttcgcggcg cgcgcgagtt gcccgttgcc ttccagctta tgaagaatgc
     6961 gctcataaat actgaaatgt ccggcagaca agtaatcgac caggctctga caaaaatcat
     7021 caagggcttt ttcgtttagc ctcatgtacg attctttgcc aggcttaatg ccaaccagat
     7081 tgtagtaagc cacgagcaga tgcttacgta catgtagcca gcgatcaacc agtttgttac
     7141 ttcctctgac gcgctccgtc aggttatcga gctggttaag catgattgac tccgcaagtt
     7201 tgtattcaaa aactgctcag tgagaaatgt aaaaaccatg ttaaacatgc cagtgatgca
     7261 aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt agatcacggc tggtgggtcg
     7321 tcagccatga acaaaaatta tggttgccga agggagtatt gccatatggc gaagcggcaa
     7381 atttcgatct tgtgggtcag cgcgcactac aaatcggcga atggcagggg gaacctgttt
     7441 ggttagtaca acagcagcgg cgtcacgata tggggtcggt acgtcaggtc attgatctcg
     7501 atgttgggct gtttcaactg gccggacgag gcgtacaact ggcggagttt taccgatcgc
     7561 ataaatactg tggttactgc gggcatgaaa tgtatccgag caaaaccgaa tgggcgatgc
     7621 tgtgcagcca ttgccgtgag cgttactatc cgcaaatcgc cccctgcatt attgttgcca
     7681 tccgtcgcga tgattcgatc ctcctcgccc agcatacccg ccatcgtaac ggtgtccata
     7741 cggtacttgc cggattcgtc gaagtgggcg aaaccctcga gcaggcagtc gcgcgggaag
     7801 tgatggaaga gagcggaatt aaagttaaaa acttgcgtta tgtgacttcc cagccgtggc
     7861 cgtttcctca gtctttaatg accgcgttta tggcggaata cgacagcggc gacatcgtga
     7921 tcgacccgaa agaattgctc gaggcgaact ggtatcgcta tgacgatttg ccgttactcc
     7981 cgccgcccgg caccgtagcg cgccgtctga tagaagatac ggtggcgatg tgtcgggcag
     8041 agtatgagtg atgatacact gaccgcctga cgcactaagg aacagccaaa atgaccgaac
     8101 ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc cgttgatgtc actccagtat
     8161 ggatgatgcg ccaggcgggt cgctatctac cggaatataa agccacgcgc gcccaggcgg
     8221 gcgattttat gtcgctgtgc aaaaacgccg agctggcgtg cgaagtgact ttgcaaccgc
     8281 tgcgtcgcta cccgctggat gcggcgatcc tcttttccga tatcctcacc gtcccggacg
     8341 cgatggggtt agggctctat tttgaagccg gagaaggtcc gcgttttacc tcgccagtca
     8401 cctgcaaagc tgacgtcgat aaactgccaa ttccggaccc ggaagatgaa ctggggtacg
     8461 tgatgaacgc ggtgcgtacc attcgtcgcg aactgaaagg cgaagtgccg ctgattggtt
     8521 tttccggcag cccgtggacg ctggcgacct acatggtgga aggcggcagc agcaaagcgt
     8581 tcaccgtgat caaaaaaatg atgtatgccg atccgcaggc gctgcacgct ctactcgata
     8641 aactggcgaa aagcgtcact ttgtatctga atgcgcagat taaagccggt gctcaggctg
     8701 tgatgatttt cgacacctgg ggcggcgtgc ttaccgggcg cgattatcaa cagttctcgc
     8761 tttattacat gcataaaatt gttgatggtt tactgcgtga aaacgacggt cgccgcgtac
     8821 cggtcacgct gtttaccaaa ggcggcggac agtggctgga agccatggca gaaaccggtt
     8881 gcgatgcgct gggcctcgac tggacaacgg acatcgccga tgcgcgccgt cgcgtgggca
     8941 ataaagtcgc gttgcagggt aatatggatc cgtcgatgct gtacgcgccg ccagcccgca
     9001 ttgaagaaga agtagcgact atacttgcag gtttcggtca cggcgaaggt catgtcttta
     9061 accttggtca cggcattcat caggatgtgc cgccagaaca tgctggcgtg tttgtggagg
     9121 cagtgcatcg gttatctgaa cagtatcacc gctaagagcc tggtccatca ggctatttta
     9181 tttgccattt tgaggccggg cagtgctcaa aatcctcacg tactacgtgt acgctccggt
     9241 ttttccgcgc tgtccggcct caaactgcct tcaacaatta cgcctggtgg accaggctct
     9301 aaggagcgat tatggatctc gcgtcattac gcgctcaaca aattgaactg gcttcttctg
     9361 tgatccgtga ggatcgactc gataaagatc cgccggatct gatcgccgga gccgatgtcg
     9421 ggtttgagca gggcggagaa gtgacgcgag cggcgatggt gctgctgaaa tacccctcgc
     9481 ttgagctggt ggagtataaa gtggcccgca tcgccaccac catgccttac attccaggtt
     9541 ttctttcctt ccgcgaatat cctgcgctgc tggcagcgtg ggagatgctg tcgcaaaagc
     9601 cggatttagt gtttgtcgat ggtcatggga tctcgcatcc tcgccgtctt ggtgtcgcca
     9661 gccacttcgg tttgctggtg gatgtgccga ccattggcgt ggcgaaaaaa cggctctgcg
     9721 gtaaattcga accgctctcc agcgaaccgg gcgcgctggc cccactgatg gataaaggcg
     9781 agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa cccgttgttt atcgctaccg
     9841 gtcatcgggt cagcgtggac agcgcgctgg cgtgggtaca acgctgcatg aaaggctatc
     9901 gtctgccgga gccaacgcgc tgggcggacg cggtggcctc ggaacgtccg gcgttcgtgc
     9961 gctatacagc aaatcagccc taattcaggt aaactgcgga taatttccgt atttgagaac
    10021 tcaacatgtt acaaaaccca attcatctgc gtctggagcg cctggaaagc tggcagcatg
    10081 tcactttcat ggcttgctta tgcgaacgca tgtaccccaa ttacgccatg ttctgccagc
    10141 aaaccggttt tggtgatggg caaatttacc gtcgtattct cgatctcatc tgggaaacgc
    10201 tgaccgttaa agacgcaaaa gtaaatttcg acagccaact ggagaaattt gaagaagcga
    10261 ttccttcagc cgacgatttc gatctgtacg gcgtttatcc ggcaatcgat gcctgcgtgg
    10321 cgttaagtga actggtccat tcgcgtttga gtggtgaaac gctcgaacac gcggtggaag
    10381 tgagtaagac ctccatcacg accgttgcga tgctggaaat gactcaggct ggtcgcgaaa
    10441 tgagcgatga agagctcaaa gaaaacccag ctgtagagca agaatgggac attcagtggg
    10501 aaatattccg acttttagct gagtgcgaag aacgcgatat cgagctgata aaaggcctta
    10561 gggcagacct gcgtgaggcg ggtgagagca atattggtat aatttttcag caataagacc
    10621 agaaaacgtg atttaacgcc tgatttgtcg tacctggagt cttccctttc gccccccgtc
    10681 tggtctacat ttggggggcg aaaaaaagtg gctatcggtg cgtgtatgca ggagagtgct
    10741 attctggcat ttccgtcgca ctcgatgctt agcaagcgat aaacacattg taaggataac
    10801 ttatgaacaa gactcaactg attgatgtaa ttgcagagaa agcagaactg tccaaaaccc
    10861 aggctaaagc tgctctggag tccactctgg ctgcaattac tgagtctctg aaagaaggcg
    10921 atgctgtaca actggttggt ttcggtacct tcaaagtgaa ccaccgcgct gagcgtactg
    10981 gccgcaaccc gcagaccggt aaagaaatca aaattgccgc agctaacgta ccggcatttg
    11041 tttctggcaa ggcactgaaa gacgcagtta agtaagattg cgtggcagtg aacagtttta
    11101 acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg atcattgatg ctggcaggtg
    11161 cgctgtttct cactgcctgt agccacaact cttcacttcc tccctttacc gccagtggat
    11221 ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga cagcggcgat aatgtgcatc
    11281 tgcttgccgt gtttagcccg tggcgcagtg gcgataccac cacgcgagag tatcgctggc
    11341 agggcgataa ccttacgctc atcaatatca atgtttacag caaaccgccg gtgaacattc
    11401 gcgcgcgttt tgacgatcgc ggcgatctga gctttatgca acgtgaatcc gatggggaaa
    11461 agcagcagct ttctaacgac caaatcgatt tataccgtta tcgtgctgat cagatccgcc
    11521 agattagcga tgccttacgt caggggagag tcgtgctgcg ccaggggcgc tggcatgcga
    11581 tggaacagac cgtgaccacc tgcgaagggc aaaccattaa acctgattta gattcgcagg
    11641 cgatagcgca tatcgagcgc cgccagagcc gctcttctgt tgatgtcagc gtggcatggc
    11701 tggaagcgcc cgaaggatcg caattactgt tagtggcaaa ctctgatttc tgtcgctggc
    11761 aacccaacga gaaaacgttc tgatttacca gtggcccata cccatatgac cgccaccact
    11821 gcagccgccg tagcccatgc ccattccggc tccgcgcgga atacccgctt cagccatcgc
    11881 aatatcccgt ttcacccgta actcatctaa cgactgacgc aaattctcca tctctttggc
    11941 gaccgcgtta attttgctgc tatccggtgg gttcgcggct aacagggcat tgtattcata
    12001 acgcttcgtc accagttgct gttgcagtgc gctgctttga gcgtaaaagt cattatggat
    12061 tttctgccac gctgtctctt gttcgctggt caaaggcgcg gcattttgct gccacatacc
    12121 gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc attgctgaaa gcgccatcat
    12181 caccagggca attttcgtgt tccgtttcat ggttaatcct ccagtggttg tcttacttcg
    12241 ggtattgcat cttccgtgcc aacgacgaaa cgctgatatg acgggtaatc tggcatgata
    12301 aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca tttttactca ttgaaactta
    12361 agtctttgtg ttacagcgca gggtaagcgc tgctaaaaga tggcatgatt tctgctgtca
    12421 aaaagggatg aacaggcaaa gaagaagata cgttttatgc aacgttcaaa agacacctta
    12481 gccaaatggt taagcgcgat cctccccgtg gtcattgttg ggctggtggg attgtttgcg
    12541 gtaacggtga ttcgtgatta tgggcgggca agtgaggctg accgtcaggc attactggaa
    12601 aaaggtaatg tgctcattcg cgctctggag tcaggaagcc gcgtagggat ggggatgcga
    12661 atgcaccatg tgcagcaaca ggcgcttctg gaagagatgg caggccagcc tggggtgttg
    12721 tggtttgcgg tcaccgatgc gcagggcatc atcattcttc acagtaaccc cgaaatggtc
    12781 gggcgtgcac tctattctcc tgatgaaatg cagcagttaa agccagagga aaactcccgc
    12841 tggagactgc ttgaggatgc agaaaaaacg cctgcgcttg aggtctatcg tttgttccag
    12901 ccaatgtcat tgccctggcg gcatggtatg cacaatatgc cgcgctgtaa cggcaaagct
    12961 gtgccacagg cagaagcaca acaggctatt ttcatcgccg ttgacgccag cgatctggtt
    13021 gcaacccaga gtggggaaaa gcgcaatacc ctgattatcc tcttcgccct ggcgacggtc
    13081 ttgctggcaa gcgtgttgtc attcttctgg tatcgccggt atctgcgctc gcgccagctt
    13141 ctacaagatg aaatgaagcg caaagagaag ctggtggcac tggggcatct ggcggcaggc
    13201 gttgcccacg aaatccgtaa cccactttcc tcgattaaag ggctggcgaa atactttgcc
    13261 gaacgcgcgc ctgcaggggg agaagcgcat caactggcgc aagtgatggc gaaagaggct
    13321 gaccgtttaa accgcgtggt aagtgagttg ctggaactgg ttaagccaac gcatctggct
    13381 ttgcaggcgg tggatctcaa cactctgatt aaccactcat tacagctggt aagccaggat
    13441 gcaaacagcc gggagatcca gttacgcttt accgccaacg acatgttgcc ggaaattcag
    13501 gccgatccgg acaggctgac tcaggtcctg ttgaatctct atctcaatgc tattcaggcg
    13561 attggtcagc atggcgtgat tagcgtgacg gccagcgaaa gcggcgcagg cgtgaaaatt
    13621 agcgttactg atagcggtaa gggaattgcg gcggatcagc ttgaagctat cttcaccccg
    13681 tacttcacca ccaaagccga cggcaccggg ctggggctag cggtcgtgca taatattgtt
    13741 gaacaacacg gtggtacaat tcaggtcgca agccaggagg gagaaggcgc aacgttcacc
    13801 ctctggcttc cggtcaatat tacgcgtaag gacccacaag gatgacgcac gataatatcg
    13861 atattctggt ggtagatgat gacattagcc actgcactat tttgcaggca ttgctgcgcg
    13921 gctggggcta taacgtcgcg ctggcgaata gtgggcaaca ggcgctggcg caggtgcggg
    13981 aacaggtttt tgatcttgtg ctttgcgatg tgcgaatggc agagatggac ggcatcgcca
    14041 cactaaaaga gatcaaagcg ttaaacccgg caattccggt actgattatg actgcgtact
    14101 ccagcgttga gacggcagta gaggcgctga aaaccggggc gctggattat ctcatcaagc
    14161 cgctggattt cgataacctg caggcgacgc tggaaaaggc gctcgcacat atgcacagta
    14221 ttgacgctga aacgcctgcg gtgtctgcca gccagttcgg gatggtcggt aaaagtccgg
    14281 cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc atcggaagcc acggtgctga
    14341 tccacggcga ttccggcacc ggtaaagagc tggtcgccag ggcgattcac gccagtagcg
    14401 cacggagtga aaaaccactg gtaacgctca actgtgcggc actcaacgaa tccttgctgg
    14461 aatctgaatt gttcggtcac gaaaaagggg cgtttaccgg agccgacaaa cggcgggaag
    14521 ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga tgaaattggc gatatctcgc
    14581 cgatgatgca ggttcgtctg ctgcgtgcga ttcaggagcg cgaagttcag cgtgtcggca
    14641 gcaaccagac catctcggtt gatgtccggc tgattgcggc gactcatcgc gatcttgccg
    14701 cagaggtgaa tgccggacgt tttcgccagg atctctacta tcgcctgaac gtggtggcga
    14761 ttgaagttcc gtcgctgcgt cagcggcggg aagatatccc tctgctggct ggccattttc
    14821 tgcagcgctt tgccgagcgt aatcgcaagg cggtaaaagg ttttacgccc caggcgatgg
    14881 atctgctgat tcactacgac tggccgggaa atattcgcga gctggaaaac gcggtggaac
    14941 gggctgtggt gctactgacc ggggaatata tttccgaacg cgagctgccg ctggcgattg
    15001 cgagtacgcc gatcccgctg gcgcaaagtc aggatattca gccgctggtg gaagtcgaaa
    15061 aagaagtgat tctggcggca ctggagaaaa cgggcggcaa caaaaccgaa gccgcccgtc
    15121 agttagggat cacgcgtaaa acgctattgg caaaactgtc gcgttagttc tgctcgcgtt
    15181 cgatagcgcg ccagccgata tctttccggc agaagcagtc gtcccaatgg atatcggtca
    15241 ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg acccagcgcg gtgacgcaca
    15301 gtacgcgccc gccgctggtc actacctgct cgtcatccgc cagtttcgtg cccgcgtgga
    15361 acactttgcc gtcagacact tcttccagcg gcaggccgtg gatcacatcg ccggtgcggt
    15421 aatctcccgg atatccaccc gcagccatca ccacgccgag agaagcgcgt tcatcccact
    15481 cggacgtttt ctcgtccagc ttgccttcac aggctgccag gcagagctca accagatcgg
    15541 acttaatgcg cagcataatc ggctgggttt ccggatcgcc aaagcggcag ttaaattcga
    15601 taaccttcgg attgccctgt ttgtcgatca tcaggcccgc gtagagaaaa ccggtgtagg
    15661 tgttgccttc cgctgccata cctttcacgg ttggccagat gatacgtttc atggtgcgct
    15721 gatgaacctc atcggtcacc actggcgcag gggagtaagc ccccatcccg ccggtgttcg
    15781 gtccggtatc tttatcaccc acgcgtttgt ggtcctgact ggtggccatc ggcagcacat
    15841 gctcgccgtc caccatcacg ataaagctcg cttcttcgcc gtcgaggaac tcttcgataa
    15901 caatgcgatg ccccgcgtcg ccaaaagcgt tgcccgccag catatcgtga acagccgctt
    15961 cggcttcttc cagcgtcatc gcgacgataa cgcctttccc ggcagccaga ccgtcagctt
    16021 taatgacgat tggcgcgccc ttctcacgca gatacgccag cgcaggttct acctcggtga
    16081 agttctggta ttccgccgta ggaatgttat ggcgggccag gaaatccttg gtaaacgctt
    16141 tcgagccttc cagctgcgcg gcacctgcgg ttgggccgaa gattttcagc ccggcggcgc
    16201 ggaaggtatc caccacgcct ttcaccagcg gcgcttccgg gccgacgatg gtcagatcga
    16261 tcttttcgtt ttgcgcgaaa tccagcagcg ccgggatatc ggttacgccg atagcgacgt
    16321 tttgcagtgt gggttccagc gcagtgcctg cattacccgg agcaacaaaa acagtttcaa
    16381 ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc gcgcccgccg ttaccaatca
    16441 ctaatacttt catctgtcgc tccattaatg gcggaaatgg cgcatgtcgg tgaagagcat
    16501 cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca tcacggatag aaccgccagg
    16561 ctggattacg caggtcacgc ccgcagcggc ggcggcatca ataccgtcgc ggaacgggaa
    16621 gaacgcgtca gaagccatcg aggaaccttt cacttccagg ccttcatcgg ccgctttaat
    16681 accggcgatt tttgcggagt acacgcggct catctggccc gcgccaatac cgatggtcat
    16741 attgtttttg gcatagacaa tggcgttgga tttcacgaac ttcgccactt tccagcagaa
    16801 cagcgcatca cgcagttcct gttcggtcgg ctgacgtttg gtgacgacgc gcagttcttc
    16861 tgcaccgacc atccccaggt ctcgatcctg aaccagcaga ccgccgttca cgcgtttgaa
    16921 atcaaggccc ggaacacgct cgccccactg accgcaggtc agaacgcgta cgttctgctt
    16981 ggcggcggtg attttcaggg cttcttcgct ggcggacggc gcaataatca cttcgacgaa
    17041 ctgacgagaa atgatggcct gtgcggtttc cgcatccagc tcgcggttaa aggcgataat
    17101 gccgccgaat gcggaggtcg ggtcggtttt gtacgcgcga tcgtaagcat caagaatgga
    17161 attgccgata gccacgccgc aagggttggc gtgcttcaca atcacacatg ccggctcggc
    17221 gaactctttc acgcactcca gcgccgcatc ggtatcggcg atgttgttat aagagagggc
    17281 tttaccctga acctgggttg cggtagcaac ggaggcttct ttcacattct cttctatata
    17341 gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata tcctgcttct taatgaagtt
    17401 caggttcagc gtgcgtggga agcgaccggc ggcttctttg ctttcaccgt ggtaagccgg
    17461 aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag gcggcggtgt gttcgaaggc
    17521 tttgatggca aggtcaaagc gagtttccag agtcagggag ccttcattgg catctatctc
    17581 tttaataatg gcgtcgtagt cgctgctctt caccacgatg gcgacatctt tatggttctt
    17641 ggcggcagag cgcaccatcg ttgggccgcc gatatcgatg ttctcaaccg catcttccag
    17701 cgagcaacct tcacgggcaa cggtctgggc gaacggatac aggttaacaa ccaccatatc
    17761 gataggctgg atctgatgtt cttccataat ggcatcgtcc tggccgcgac ggcccagaat
    17821 gccaccatgt actttcggat gcagggtctt cacgcgtcca tccatcatct ccgggaaacc
    17881 ggtgtaatcg gaaacttcgg ttaccggcag acctttttct gctaacagac gggcagtgcc
    17941 ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc tgggcgaatt cgacgatacc
    18001 ggctttgtca gaaacactga gcagagcgcg gcggactgga cgacgttgtt gcatggtaaa
    18061 tcccctggat ttgactatta cagagagcgt tagctgaatt tttcgcgaaa aactcagcta
    18121 acgcccctaa cggggcatcc ttatttttcg cccgcattgt aacgaaaacg tttgcgcaac
    18181 gctcgcgaat ttttctcttt caatggtgat cacaattttg acagtggttg ccgtgggaaa
    18241 aatacagaaa ttacattgat gattgtggat aactctgtgc gtaaaaaggt ataaagcggg
    18301 gttttgctgg ggaatgcagc agtcagtcat ttttctgcaa tttttctatt gcggcctgcg
    18361 gagaactccc tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca
    18421 gccggttcgg ttgaagagaa aaaatcctga aattcagggt tgactctgaa agaggaaagc
    18481 gtaatatacg ccacctcgcg acggtaagct gaa
//
LOCUS       DAPOZO010000092        18307 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPOZO010000092 DAPOZO010000000
VERSION     DAPOZO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.092"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            207..1742
                     /gene="gadC"
                     /locus_tag="ROI21_003595"
     CDS             207..1742
                     /gene="gadC"
                     /locus_tag="ROI21_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDX5112472.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAVVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKGNTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1873..3192
                     /locus_tag="ROI21_003596"
     CDS             1873..3192
                     /locus_tag="ROI21_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDX5112473.1"
                     /translation="MDICSRNEKLAIRRPAVLVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAIIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3490..3531
                     /locus_tag="ROI21_003597"
     CDS             3490..3531
                     /locus_tag="ROI21_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112474.1"
                     /translation="MVQLVDLARCVSF"
     gene            3569..4951
                     /gene="dosC"
                     /locus_tag="ROI21_003598"
     CDS             3569..4951
                     /gene="dosC"
                     /locus_tag="ROI21_003598"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDX5112475.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASLLSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4976..7375
                     /gene="dosP"
                     /locus_tag="ROI21_003599"
                     /gene_synonym="pdeO"
     CDS             4976..7375
                     /gene="dosP"
                     /locus_tag="ROI21_003599"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDX5112476.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKIYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNARSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7633..8214
                     /gene="ddpX"
                     /locus_tag="ROI21_003600"
     CDS             7633..8214
                     /gene="ddpX"
                     /locus_tag="ROI21_003600"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDX5112477.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGIQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAEFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHAS"
     gene            8228..9778
                     /locus_tag="ROI21_003601"
     CDS             8228..9778
                     /locus_tag="ROI21_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDX5112478.1"
                     /translation="MKRSISFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVRFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKMTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9780..10802
                     /locus_tag="ROI21_003602"
     CDS             9780..10802
                     /locus_tag="ROI21_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5112479.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVDNIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10799..11695
                     /locus_tag="ROI21_003603"
     CDS             10799..11695
                     /locus_tag="ROI21_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDX5112480.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATYNPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11692..12678
                     /locus_tag="ROI21_003604"
     CDS             11692..12678
                     /locus_tag="ROI21_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5112481.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGHISLLGEDALNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMP
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNHSCACWYPQQEVISV"
     gene            12671..13597
                     /locus_tag="ROI21_003605"
     CDS             12671..13597
                     /locus_tag="ROI21_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5112482.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYICSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDVQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     RERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(13653..14084)
                     /gene="osmC"
                     /locus_tag="ROI21_003606"
     CDS             complement(13653..14084)
                     /gene="osmC"
                     /locus_tag="ROI21_003606"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDX5112483.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14429..14644
                     /gene="bdm"
                     /locus_tag="ROI21_003607"
     CDS             14429..14644
                     /gene="bdm"
                     /locus_tag="ROI21_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDX5112484.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            14746..14883
                     /gene="sra"
                     /locus_tag="ROI21_003608"
     CDS             14746..14883
                     /gene="sra"
                     /locus_tag="ROI21_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDX5112485.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15040..16737
                     /gene="maeA"
                     /locus_tag="ROI21_003609"
     CDS             15040..16737
                     /gene="maeA"
                     /locus_tag="ROI21_003609"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDX5112486.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            16871..17881
                     /gene="adhP"
                     /locus_tag="ROI21_003610"
     CDS             16871..17881
                     /gene="adhP"
                     /locus_tag="ROI21_003610"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDX5112487.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            18027..>18307
                     /locus_tag="ROI21_003611"
     CDS             18027..>18307
                     /locus_tag="ROI21_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDX5112488.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVT"
BASE COUNT         4575 a         4509 c         4691 g         4532 t
ORIGIN      
        1 cacccgaaac tgcagggtat tgcccagcag aacagcttta aacatacctg ataacgttta
       61 acggtaacgg tgtcccgaaa cacacccgtt tcgggacaat ttccaaagtc tgttcactgg
      121 cattagcaac ggaaaatatt gttctgaata cgcttcagaa caaaacaggt gcggttccga
      181 caggaatacc gttttagggg gataatatgg ctacatcagt acagacaggt aaagctaagc
      241 agctcacatt actcggattc tttgccataa cggcatcgat ggtaatggct gtttatgaat
      301 accctacctt cgcaacatcg ggcttttcat tagtcttctt cctgctatta ggcgggattt
      361 tatggtttat tcccgtggga ctttgtgctg cggaaatggc caccgtcgac ggctgggaag
      421 aaggtggtgt cttcgcctgg gtatcaaata ctctggggcc gagatgggga tttgcagcga
      481 tctcatttgg ctatctgcaa atcgccattg gttttattcc gatgctctat ttcgtgttag
      541 gggcactctc ctacatcctg aaatggccag cgctgaatga agaccccatt accaaaacta
      601 ttgcagcact catcattctt tgggcgctgg cattaacgca gtttggtggc acgaaataca
      661 cggcgcgaat tgctaaagtt ggcttcttcg ccggtatcct gttacctgca tttattttga
      721 tcgcattagc ggctatttat ctgcactccg gtgcccccgt tgctatcgaa atggattcaa
      781 agactttctt ccctgacttc tctaaagtgg gcaccctggt tgtatttgtt gccttcattt
      841 tgagttatat gggcgtagaa gcatccgcaa cccacgtcaa tgaaatgagc aacccagggc
      901 gcgactatcc gttggctatg ttactgctga tggtggcggc aatctgctta agctctgttg
      961 gcggtttgtc tattgcgatg gtcattccgg gtaatgaaat caacctctcc gcaggggtaa
     1021 tgcaaacctt taccgttctg atgtcccatg tggcaccgga aattgagtgg acggttcgcg
     1081 tgatctccgc actgctgttg ctgggtgttt tggcggaaat cgcttcctgg attgttggtc
     1141 cttctcgcgg gatgtatgtc actgcgcaga aaaacctgct gccagcggca ttcgctaaaa
     1201 tgaacaaaaa tggcgtaccg gtaacgctgg tcatttcgca gctggtgatt acttctatcg
     1261 cgttgatcat cctcaccaat accggtggcg gtaacaacat gtccttcctg atcgcactgg
     1321 cgctgacggt ggtgatttat ctgtgtgctt atttcatgct gtttattggc tacattgtgt
     1381 tggttcttaa acatcctgac ttaaaacgca catttaatat ccctggtggt aaaggggtga
     1441 aactggtcgt ggctgttgtc ggtctgctga cttcaattat ggcgtttatt gtttccttcc
     1501 tgccgccgga taacatccag ggtgactcta ccgatatgta tgttgaatta ctggtcgtga
     1561 gtttcctggt ggtacttgcc ctgcccttta ttctctatgc tgttcatgat cgtaaaggca
     1621 aagggaatac cggcgtcact ctggagccaa tcaacagtca gaacgcacca aaaggtcact
     1681 tcttcctgca cccgcgtgca cgttcaccac actatattgt gatgaatgac aagaaacact
     1741 aaccatgtct gaatggttat aagataaggg agcgataatt catcgctccc tttttcgtgc
     1801 ttgctgtcgt attgacttaa ccgggataag tacgagaatg agcgcacatc tgtttaccgg
     1861 aaaccagcac atatggatat ctgctcccga aacgagaaat tagcgattag aagaccagcg
     1921 gtactggttg cactggcact tttactgtgt agttgcaaaa gcacgcctcc agagtcaatg
     1981 gtgacaccac cagcgggttc aaagccacca gccacgacgc aacaatcgtc acaaccgatg
     2041 cgtggcatct ggctggccac ggtgtctcgc ctcgactggc caccggtttc ctcggttaac
     2101 attagtaacc ccaccagccg ggcccgtgta caacaacagg cgataatcga caaactggat
     2161 catctgcaac gtctcggcat caacacggtc tttttccagg tcaagccaga cggtaccgcc
     2221 ctgtggccgt cgaaaatttt gccgtggtcc gatcttatga ccggtaagat tggtgaaaat
     2281 ccgggttacg atccgctgca attcatgctc gacgaagccc acaagcgtgg gatgaaagtt
     2341 cacgcctggt ttaaccccta tcgcgtatcg gttaatacaa agcccggtac tatcagggaa
     2401 ctgaatagca ccctgtctca acaaccggcg agcgtctatg tgcaacaccg cgactggatc
     2461 agaacgtccg gcgatcgctt tgtcctcgac ccgggcatac ctgaggttca ggactggatc
     2521 acatcaatag tcgcagaagt ggtttcccgc tatccggtag atggcgtgca gtttgacgac
     2581 tatttctata ctgagtcacc gggttcacgg ctaaatgata acgaaacgta ccgcaaatac
     2641 ggaggcgcat ttgcgtcaaa agcagactgg cggcgcaaca atactcagca gttaattgcg
     2701 aaagtatcgc acaccattaa aagcattaag ccggaagtcg aatttggcgt tagcccggca
     2761 ggcgtgtggc gtaaccgatc acacgatccg ctcggttccg atacccgagg cgcggcagcc
     2821 tatgacgaat cctacgctga cacccgtcga tgggtggaac aaggattgct ggattacatt
     2881 gctccccaaa tttactggcc gttctcacgg agtgccgcac gttatgacgt tttggcaaaa
     2941 tggtgggcgg atgtcgttaa accgaccagg acccgcctgt atatcggtat cgccttctat
     3001 aaagtgggtg aaccttcaaa gatagagcca gactggatga ttaacggcgg cgtaccggaa
     3061 ctgaaaaaac agctcgatct taacgatgcc gtgccagaaa ttagcggcac aatcttgttc
     3121 cgtgaggact atctgaataa accgcagact caacaagcgg ttagctatct gcaaagtcgt
     3181 tggggcagtt aaactcccat tcatactttg tgacaagccg gtggtttacc caccggcttg
     3241 ctaccctctt gaaatatccc actagtaatc atgcttactt aagtcaaatt aactacgcaa
     3301 ttaaccacac ttagttaaac agtcataaat cccctaataa aaatgtatga atatgaaaaa
     3361 acatacgata ttcgctgtca ccgaaaaata attacgatgg gctatactct tctgaagaac
     3421 agtagcgtag attgaaactg tcctaagtcg cttatttgtt ttcagtatat cttcatattt
     3481 caggagaata tggtgcagtt agtcgatctc gcacgttgcg tttcgttttg attccgtctg
     3541 ctttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa tgaaagatga
     3601 gtggaccggg cttgtcgaac aagcagatcc gctcattcgt gctaaagccg cggaaattgc
     3661 cgttgcgcat gcccattatc tgagtattga gttttaccga attgtccgta tcgacccgca
     3721 tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg cgatggaacg
     3781 ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa tacaaatcca
     3841 gcataccgtt gcggaagtgc atgcccgcat aggaattccg gtagaaattg tcgagatggg
     3901 gtttcgggtg ctgaaaaaaa tcctctatcc ggtcatcttc tcttcggatt attccgccgc
     3961 agaaaaactt caggtctacc atttctctat taacagtatt gatatcgcta tggaagtgat
     4021 gacccgcgcg tttaccttta gtgacagtag tgcctcaaaa gaagatgaaa actatcgtat
     4081 cttctcgtta ctggaaaatg ccgaagaaga aaaagagcgg caaatagcct cattactttc
     4141 atgggaaata gatattatct ataaagtcct gctggattct gatttaggca gtagtttgcc
     4201 tttaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt attttagcgg
     4261 catagccgaa gtgggccata tctcccgtct gattcaggat tttgacggta ttttcaatca
     4321 aaccatgcgt aacaccagga atttgaataa cagaagttta cgggtgaaat ttttattaca
     4381 gataagaaat accgtatcgc aaattattac cttgttgcgt gaattgtttg aagaagtatc
     4441 gcgccacgaa gtcggtatgg atgtactgac gaaattactt aaccgccgtt tcctgccgac
     4501 tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt cagtgctgat
     4561 tattgacgtt gataaattca aagagattaa cgatacgtgg ggccataaca ctggcgatga
     4621 aattctgcgt aaagtctctc aggcctttta tgacaacgtc cgcagtagtg attatgtttt
     4681 ccgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttcggaaa acgaaacgtt
     4741 acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag ccgcaaacgg
     4801 cgaagatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc atcctgacta
     4861 tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac gaggtagaaa
     4921 ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg taatcatgaa
     4981 actaaccgat gcggataatg ccgccgatgg catttttttc cccgcccttg agcaaaatat
     5041 gatgggcgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc ccgccgcaga
     5101 gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata tgctgattcc
     5161 gcgggatttg cgtcccgcgc atcctgaata catccgtcac aaccgtgaag gcggtaaagc
     5221 gcgcgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca gtaaaatctg
     5281 gacccgtttt gcgctatcga aagtgagcgc cgagggtaaa atttattacc tggcgctggt
     5341 acgggatgcc agcgtagaaa tggcgcaaaa agaacagacc cgacaattga ttattgccgt
     5401 tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg tgcagtgcaa
     5461 tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta tgcagcccga
     5521 tacactcctg aacattccag aattccctgc tgataaccgc attcgtttac aacagttgct
     5581 atggaaaacc gcccgcgatc aggacgaatt tctgctgttg acgcgcaccg gtgaaaaaat
     5641 ctggattaaa gcctctatca gcccggtcta tgatgtgctc gcgcatctgc agaacctggt
     5701 aatgactttc tcggatatca ccgaagaacg gcaaatccgc cagcttgaag gcaatattct
     5761 cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaattattt gtcgtaacat
     5821 cgaatctgta ctcaacgaat cgcatgtttc gctgttcgcg ctgcgcaacg ggatgccgat
     5881 acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcgaagct ggtcagcgac
     5941 cattcgtcag cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct cgtcaggagc
     6001 agaaaccagc gcctttatcg aacgcgtggc agatatcagc cagcatatgg ccgcgctggc
     6061 gctggaacag gaaaaaagcc gtcagcatat tgaacaactc atccaatttg atccgatgac
     6121 cggtctgcca aatcgcaata acctgcacaa ttacctcgat gacctggtcg acaaagccgt
     6181 ctctccggtg gtctatctca tcggtgttga ccatattcag gatgtgattg atagccttgg
     6241 ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg aaaaactcaa
     6301 accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga gcctcgaaaa
     6361 cgacgtcagt aacattaccc agatcgccga tgagctacgg aatgtggtca gcaagccgat
     6421 aatgattgac gataaaccat tcccgcttac cttgagtatt ggcatcagct acgacgtggg
     6481 taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata ttcgcaaaaa
     6541 tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa aagagcgttt
     6601 ggttttaggc gcggcgctga aagaagcaat tagcaataac caactaaaac tggtttacca
     6661 gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg ctcgctggca
     6721 cgatcccctg catggtcatg tgcccccttc acggtttatt cctctcgcag aagagatcgg
     6781 tgaaatcgaa aatattggac gctgggtcat cgcggaagct tgccgtcagt tagcagaatg
     6841 gcgtagccag aatattcata tcccggcgtt atccgttaac ttgtcggcgt tgcactttcg
     6901 cagtaatcag ctgcctaatc aggtgtctga tgcaatgcac gcctggggta ttgacggcca
     6961 ccagctgaca gtggaaatca cggaaagcat gatgatggaa cacgataccg aaatctttaa
     7021 gcgcattcag atcctgcgcg atatgggcgt gggtttatcg gtagatgatt ttggcacggg
     7081 cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa ttgacaaaag
     7141 ttttgtcgat cgttgtctga ccgaaaaacg catccttgcc ttacttgaag ccattaccag
     7201 cattgggcaa agccttaatt taaccgtcgt ggcggaaggc gtcgaaacca aagagcaatt
     7261 tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt cccgccccct
     7321 gcccgccgaa gaaattccag gctggatgag cagtgtgtta ccgctgaaaa tctgacaaat
     7381 tcctctcgcc cgcactcgcg ggttttcctt taacgtgaca ctgtcactta aatactgtga
     7441 tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc aaaactgcac
     7501 tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag cattctggcg
     7561 cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt ttgtcttata
     7621 caggaactgt ttatgtcgga taccaccgaa ctggttgatt tagccgtgat cttccctgat
     7681 ctggagatcg aactgaaata cgcctgcgct gataacatca caggtaaagc tatttatcag
     7741 caagcgcgtt gtctgttaca caaggatgcg attaccgcgc tggcgaaaag tatcagtatc
     7801 gcccagctgt caggcataca actggtgatt tacgatgcgt atcgcccaca acaagcacag
     7861 gcgatgttgt ggcaagcctg cccagacccg caatatgttg ttgatgtgac ggtcggttct
     7921 aatcacagcc gtggcacggc gatcgacctg acgcttcgtg atgagcacgg gaacatcctg
     7981 gatatgggtg cagagttcga tgaaatgcac gaacgttccc atgcttatca cccttccgtc
     8041 ccgccagccg ctcagcgcaa tcggctgttg ctgaatgcga ttatgactgg cggtggcttt
     8101 gtcggtatcc cgagcgaatg gtggcacttc gaattacccc aggcagcgag ttacccacta
     8161 ctcgctgatc aattcacctg ttttatatcc cctggcacac agcacgccag ttaatcagga
     8221 aactgtcatg aagagatcga tatcgtttcg tcctacattg ctcgcgatcg tccttgccac
     8281 aacaatgccg gttgcgcacg ccgccgtacc gaaagatatg ctggtgatcg gtaaagctgc
     8341 cgacccacaa accctcgacc cagcggtaac aattgataat aacgactgga cagtgaccta
     8401 cccgtcttat cagcgactgg ttcagtacaa aacggacggt gataaaggct caaccgacgt
     8461 tgaaggcgat ctggctagta gctggaaagc gtctgacgat caaaaagagt ggacgttcac
     8521 cctgaaggac aatgctaaat ttgccgatgg cacacctgtc actgccgaag cagtaaaact
     8581 ctcttttgag cggttactaa aaatcggcca ggggccagca gaagcatttc ccaaagattt
     8641 aaagattgat gctcccgacg aacatacagt gaggtttacc cttagccagc cattcgcacc
     8701 gttcctctac acgctggcga atgacggtgc atccattatc aatccggcgg tcttaaagga
     8761 gcatgcggcg gatgatgccc gtggtttcct cgcgcaaaat accgctggct ccggaccgtt
     8821 tatgctgaaa agctggcaaa aaggtcagca attagttctg gtgccaaatc cgcattaccc
     8881 cggcaataaa ccgaatttca aacgggtatc ggtaaaaatt atcggtgaaa gtgcttcccg
     8941 tcgcctgcag ctctcccgtg gcgacattga cattgccgat gcgctgccgg tggatcaact
     9001 caacgcactg aagcaggaaa acaaagtcaa cgtggcagag tatccgtcac tgcgcgtcac
     9061 ctatctgtat ctgaataaca gcaaagcgcc gcttaatcag gcggatctgc gtcgggccat
     9121 ttcctggtct accgattatc agggtatggt taacggcatt ctgagtggta acggaaaaca
     9181 aatgcgcggc ccgattccgg aaggcatgtg gggatacgat gcgacggcaa tgcaatacaa
     9241 ccatgacgaa acgaaagcca aagccgaatg ggataaaatg acgagcaaac ccaccagcct
     9301 gacgtttctc tattctgata atgatccgaa ctgggagcct attgctctgg cgacacaatc
     9361 cagtctcaac aagctgggca tcaatgtgaa gctggaaaag ctggcgaacg ccaccatgcg
     9421 cgacagagtg ggtaaaggtg attacgacat cgcgattggc aactggagtc cggattttgc
     9481 cgacccgtat atgtttatga attactggtt tgagtcagac aaaaaaggtc tgccgggtaa
     9541 ccgctcgttc tatgaaaaca gtgaggtcga taagctactg cgcaatgcgc tagcgaccac
     9601 cgaccagacg cagcgtaccc gggactacca gcaggcacag aaaatcgtca ttgatgacgc
     9661 tgcttatgtg tacctgttcc agaaaaacta ccaactggcg atgaacaaag aggtgaaagg
     9721 ctttgtgttc aatcccatgc tggaacaggt cttcaatatc aataccatga gtaaataacg
     9781 tgaccttctg gagtatttta cgccaacgct gctgggggct ggtgctcgtg gtggcgggcg
     9841 tctgtgtgat tacgtttatt atctcgcacc tgatccccgg cgatccggcg cggttactgg
     9901 cgggtgaccg cgccagcgat gctatcgtgg ataatattcg ccagcaactg ggactggacc
     9961 agccactgta tgtacagttt taccgctacg tcagcgacct gtttcagggt gacctgggaa
    10021 catccattcg taccgggcgt ccggtgctgg aagagttgcg catatttttc ccggcgacgc
    10081 tggaactggc tttttgtgcc ctgctgctgg cactcctgat tggcatcccg ctgggcatac
    10141 tctctgcagt ctggcgaaat cgctggctgg atcatctggt gcgaataatg gccattaccg
    10201 gaatctccac acctgcgttc tggcttgggc tgggcgtcat tgtgctgttt tatggtcatc
    10261 tgcaaattct tcccggcggc ggaaggcttg atgactggct ggatccacca acgcacgtta
    10321 ccggctttta tctgctcgat gcgctgcttg aaggcaacgg tgaagtcttc ttcaatgcgc
    10381 tgcaacatct catcttaccg gcattaacgc tggcgttcgt tcacctggga attgtcgctc
    10441 gccagatccg ctcagctatg ctggaacaat tgagtgaaga ttacattcgt accgcccggg
    10501 ccagcggctt gcccggctgg tatatcgttt tatgttatgc gctacccaat gcgttgatcc
    10561 cctcgattac cgtattgggt ctggcgctgg gcgatttgtt gtatggcgca gtgctcaccg
    10621 aaaccgtttt tgcctggccc ggaatgggtg catgggtagt aacatcaata caggcgctcg
    10681 acttcccggc agtgatgggc tttgccgtcg tggtttcatt tgcttatgtg ctggtcaacc
    10741 tggtggtgga tttgctctat ttgtggattg atccgcgtat cggacgtgga ggtggtgaat
    10801 gatgctaagc gaggaaacgt ccgccgtacg cccacaaaaa caaacgcgat ttaacggtgc
    10861 aaaactggta tggatgctga aaggcagtcc gcttaccgtg accggcgcag tcatcattgt
    10921 attaatgcta ctgatgatga ttttttcacc gtggctggcg acgtataatc ccaacgccat
    10981 tgatttaacc gcccgccttt tgccgccttc tgcggcgcac tggtttggca ccgatgaagt
    11041 gggacgcgat ctgtttagcc gcgtactggt cggcagtcag caatcaattc tcgccggatt
    11101 agtggtggtc gccattgcag gtatgatggg gtcgctactc gggtgtctat ccggtgtgct
    11161 tggcggacgc gcagacgcga ttatcatgcg catcatggac attatgctgt cgattccttc
    11221 gctggtgctg acaatggcac tggcagccgc tctcgggccg agtttgttta acgccatgct
    11281 ggcgattgct attgtgcgaa ttccctttta tgtgcgcctg gcgcgggggc aaacattagt
    11341 tgtccgccag tacacttacg ttcaggcggc gaaaaccttt ggtgcgtcac gctggcatct
    11401 gatcaactgg catattttac gtaactcgct gccgccgctg attgtgcagg catcgctgga
    11461 tatcggtagc gcgattttaa tggccgccac gttgggattt attggcctgg gtgctcaaca
    11521 accgagtgct gaatgggggg cgatggtggc gaatggtcgc aactatgtgc tcgatcaatg
    11581 gtggtattgc gcatttccgg gggcagcgat tttgcttacc gccgtcgggt ttaatctctt
    11641 tggcgatggt attcgcgatc tgcttgaccc gaaagcagga ggaaagcagt catgacccaa
    11701 cccgttctgg acattcaaca gctgcatttg agtttccccg gttttaacgg cgacgttcac
    11761 gcgctcaaca atgtgtcctt gcagattaac cgcggtgaaa ttgtcggtct ggtgggagaa
    11821 tccggctcgg gtaaatcagt cactgcaatg ttgattatgc gtctgctacc aacgggcagt
    11881 tattgcgtac atcggggaca tatttcactg ctgggagaag atgctcttaa cgctcgggaa
    11941 aagcagcttc gtcagtggcg cggcgcacga gtggcgatga tctttcagga accgatgacc
    12001 gcccttaatc cgacacgtcg aataggtctt cagatgatgg acgtgatccg ccatcatcaa
    12061 ccaataagtc gtcgggaagc cagagctaaa gcgattgccc tgctggaaga gatgcaaatc
    12121 ccggatgccg tggaagttat gtcgcgctat ccgtttgagc tttcaggtgg tatgcgccag
    12181 cgggtaatga ttgcgctggc attctcctgc gagccgcaat tgattattgc cgacgaaccg
    12241 actacggcgc tggacgtcac ggtacagttg caggtattgc gtctgcttaa acataaagcc
    12301 cgcgccagtg gaactgcggt actgttcatc agccatgata tgcccgtggt gtcgcaactg
    12361 tgcgatagcg tttacgtgat gtatgccgga agcgtgattg agagcggcgt gacggcagac
    12421 gttatccatc atccccggca tccgtatacc attggtttgc tgcaatgcgc accggaacat
    12481 ggaataccgc gccaaccatt acccgctatt ccggggacgg taccaaacct cacccatttg
    12541 cctgacggct gcgcttttcg cgatcgttgc tatgcggcag gtgcacagtg tgaaaacgtc
    12601 ccggcgctga cagcgtgtgg tgacaacaac cacagctgcg cctgttggta tcctcagcag
    12661 gaggtcatta gtgtctgaca cgttattaac gttacgcgac gtccatatca atttcccggc
    12721 ccgaaaaaac tggcttggta aaactacgga acatgttcat gccatcaatg gtattgattt
    12781 acagatccgt cgtggtgaaa ccttagggat cgtcggtgag tcaggctgcg gcaaaagcac
    12841 cctcgcacag cttttaatgg gtatgctgca accgagccac gggcagtaca tctgttcagg
    12901 atcacaacgc attatgcaga tggtgtttca ggacccgctc tcttcgctta atccgcgctt
    12961 accggtgtgg cgcatcatca cggaaccgct ctggattgct aagcgtagta gtgaacaaca
    13021 gcggcgagcg ttggcagagg agctggctgt gcaggtgggt attcgtccgg agtatctcga
    13081 ccgcttgcct catgcgttct caggcgggca gcggcaacgt atcgccattg ccagagcact
    13141 ctcttcgcag cctgacgtga ttgtgcttga tgaaccaacc tcggcgctgg atatctccgt
    13201 gcaggcgcag atcctcaatt tactggtaac gctacaggaa aatcgcgagc tgacttatgt
    13261 gctgatttca cacaatgtct ccgtgatacg tcatatgagc gatcgggtgg cggtgatgta
    13321 tctcggacag attgtggaac taggagacgt gcaacaggtg ctgacagctc cggcacatcc
    13381 atacacccga ttattgctgg attccctccc cgccattgat aaaccgctgg aggaagaatg
    13441 ggcattacgt aaaacggatc taccaggaaa ccgcacgttg ccacaaggct gttttttccg
    13501 cgaacgctgc cctctggcaa cccacggatg tgaagtccgg caatcattaa caataaggga
    13561 ggacggacgt gagatccggt gctggcgggc gctgtagaga cgctaacaac gcatcaggca
    13621 atcacacgcc tgacgcgtca tccggcaacg ctttacgatt tcaactggta atccagcgta
    13681 atttccgctt tcagtacctg tgaaaccgga catcctgctt ttgctttctg gatgatgccg
    13741 tcaaaggtag aggcatcaat acccggcacc gcaacttcgc tcttcagtgc gattttagta
    13801 atcgcaaaac cggcatccac tttatccagc gacacatcgg cggtggtatc aatcgatgtt
    13861 ggcgtgaatc ccgcttcccc cagcattaat gaaagcgcca ttgagaaaca tgcggcatgc
    13921 gctgcgccaa tcagttcttc agggttggtt cctttttcgc cttcaaaacg cgtgttaaat
    13981 ccatacggct gttggttcag cacgccgctc tcggtggata ctgttccctt cccgcgtttg
    14041 atatcgcctt cccagtgtgc ctgacctttc ttatggattg tcattgttgc tctcctgtgg
    14101 gcttaaaaag gaaattatag actacgtgag aaaccgcacg aggataagca ggatattccg
    14161 aataaaatcc ggcgaaaaca ttatctctgt acttaaatta aattcccaca atagataacg
    14221 gggaatatat aatccaaaag atattgtatc aatattaatc ggatcgcccc aattacaatc
    14281 cctatgaata tcatggtgtt ataatataaa aattatcaac caggactaat cttaacaacg
    14341 aagcggcaaa tatttgccat gctgaatgtg ctttataaat ctgcgatccg tagcagacac
    14401 caaaaataca cagacacgga gaatcgctat gtttacttat tatcaggcag aaaattcaac
    14461 agcagaaccc gctctggtta atgccattga gcaaggcctt cgggcagagc acggtgttgt
    14521 cactgaagat gacattctca tggagctgac caaatgggtg gaagcttctg ataacgacat
    14581 cctcagtgat atctaccagc aaacgatcaa ttatgtggtc agtggccagc accctacgct
    14641 ttaaggtgct atgcttgatc ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta
    14701 cggcgatgtt gtccttaaaa ctagcaacag gattgaggag ttaaaatgaa atcgaaccgt
    14761 caggcacgtc atattcttgg actggaccat aaaatttcta accagcgcaa aatagttacc
    14821 gaaggtgaca aatccagcgt ggtaaataac ccaaccggca gaaaacgccc cgctgaaaag
    14881 taattcataa ccatcagtcc tcaatgacga ttaaacacca ttgcctgcgc aatggtgttt
    14941 ttgtttttat ctgctttata cttggggccg acgccctggc ggtaaagcaa agacgataaa
    15001 agcgtgccag ggatggatat tcaaaaaaga gtgagtgaca tggaaccaaa aacaaaaaaa
    15061 cagcgttcgc tttatattcc ttacgctggc cctgtactgc tggaatttcc gttgttgaat
    15121 aaaggcagcg ccttcagcat ggaagaacgc cgtaacttca acctgctggg gttactgccg
    15181 gaagtggtcg aaaccatcga agagcaagcg gaacgagcat ggatccagta tcagggattc
    15241 aaaaccgaaa tcgacaaaca catctacctg cgtaacatcc aggacactaa cgaaaccctc
    15301 ttctaccgtc tggtaaacaa tcatcttgat gagatgatgc ctgttattta taccccaacc
    15361 gtcggcgcag cctgtgagcg tttttctgag atctaccgcc gttcacgcgg cgtgtttatc
    15421 tcttaccaga accgccacaa tatggacgat attctgcaaa acgtgccgaa ccataatatt
    15481 aaagtgattg tggtgactga cggcgaacgt attctggggc ttggtgacca gggcatcggc
    15541 gggatgggca ttccgatcgg taaactgtcg ctctataccg cctgtggcgg catcagtccg
    15601 gcctataccc ttccggtggt gctggatgtc gggacgaaca accaacagct gcttaacgat
    15661 ccgctgtata tgggctggcg taatccgcgt atcactgacg atgaatacta tgaattcgtt
    15721 gatgaattta tccaggctgt gaaacaacgc tggccggacg tactgttgca gtttgaagac
    15781 ttcgcacaaa aaaatgcgat gccgttactt aaccgctatc gcaatgaaat ttgttctttt
    15841 aacgatgaca ttcagggcac tgcggcggta acggtcggca cactgatcgc agccagccgc
    15901 gcagcaggtg gtcagttaag cgagaaaaaa atcgtcttcc ttggcgcagg ttcagcggga
    15961 tgcggcattg ccgaaatgat catcgcccag actcagcgtg aaggattaag cgaggaagcg
    16021 gcgcggcaga aagtctttat ggttgatcgc tttggcctgc tgaccgacaa gatgccgaac
    16081 ctgctgcctt tccagaccaa actggtgcag aaacgcgaaa acctcagtga ctgggatacc
    16141 gacagcgatg tgctgtcact gctggatgta gtgcgtaatg taaaaccaga tattctgatt
    16201 ggcgtctcag gacagaccgg gctgtttacg gaagagatca tccgtgagat gcataaacac
    16261 tgtccgcgtc cgatcgtgat gccgctgtct aacccgacgt cacgcgtgga agccacaccg
    16321 caggacatta tcgcctggac tgaaggtaac gcgctggtcg ccactggcag tccgtttaac
    16381 ccagtggtat ggaaagataa aatctaccct atcgcccagt gtaacaacgc ctttattttc
    16441 ccgggcatcg ggctgggcgt tattgcttcc ggcgcgtcac gtatcaccga tgagatgctg
    16501 atgtcggcaa gtgaaacgct tgctcagtat tcgccgctgg tcctgaacgg cgaaggtctg
    16561 gtactaccgg aactgaaaga tattcagaaa gtctcccgcg caattgcctt tgcggttggc
    16621 aaaatggcgc agcagcaagg cgtggcggtg aaaacctccg ccgaagctct gcaacaggcc
    16681 attgatgata atttctggca cgccgaatac cgcgactacc gccgtacctc catctaagcc
    16741 tgcgcccggt ggtgaaagct gccgggcaat tttcctctcc ctttttcaga tctcatccat
    16801 actgggtagt ggcgaataaa tctcatttgc ctcacctgct atgcagaaca tcatccgaaa
    16861 aggaggaact atgaaggctg cagttgttac gaaggatcat catgttgacg ttacggataa
    16921 aacactgcgc tcactgaaac atggcgaagc cctgctgaaa atggagtgtt gtggtgtatg
    16981 tcataccgat cttcatgtta agaatggcga ttttggtgac aaaaccggcg taattctggg
    17041 ccatgaaggc atcggtgtgg tggcagaagt tggcccaggt gtcacctcat taaaaccagg
    17101 cgatcgtgcc agcgtggcgt ggttttacga aggatgcggt cattgcgaat actgtaacag
    17161 tggtaacgaa acgctctgcc gttcagttaa aaatgccgga tacagcgttg atggcgggat
    17221 ggcggaagag tgcatcgtgg tggcggatta cgcggtaaaa gtgccagatg gcctggactc
    17281 ggcggcggcc agcagcatta cctgtgcagg ggtcaccacc tacaaagccg ttaagctgtc
    17341 aaaaattcgt ccagggcagt ggattgctat ctacggtctt ggcggtctgg gtaacctcgc
    17401 cctgcaatac gcgaagaatg tctttaacgc gaaagtgatc gccattgatg tcaatgatga
    17461 gcagttaaaa ctggcaaccg aaatgggcgc tgatttagcg attaactcac gcactgaaga
    17521 cgccgccaaa attgtgcaag aaaaaaccgg tggcgctcac gccgcggtgg tgacagcggt
    17581 agctaaagct gcgtttaact cggcggttga tgctgtacga gcaggcggtc gtgttgtggc
    17641 tgtcggtctg ccgccggagt ctatgagcct ggacatccca cgccttgtgc tggatggtat
    17701 tgaagtggtc ggttcgctgg tcggcacgcg ccaggatctc accgaagcct tccagtttgc
    17761 ggccgaaggc aaagtggtgc cgaaagtcgc cctgcgtccg ttagcggaca tcaacaccat
    17821 ctttactgag atggaagaag gcaaaatccg tggccgtatg gtgattgatt tccgccgcta
    17881 agaggccttt gctgcgactg ccatgttcgg gtcgcagcat cgcacactct ccaacatgaa
    17941 atggctaaat ggattattgt tgcgtaggaa ggaacaataa tgatagtgtt cctggtgaag
    18001 gaacgctact gagagtaaag acactaatga aaatggccaa tcatccccgc ccgggggaca
    18061 ttattcagga atcactggac gaacttaatg tcagcctgcg cgagtttgcc agagcaatgg
    18121 aaattgcgcc ctcaacggca agccgattgc tgaccggaaa agcagctttg acaccagaaa
    18181 tggcgattaa actttccgtg gtgatcggca gttcgccgca aatgtggctg aatctgcaaa
    18241 acgcctggag tctggcagag gcagaaaaaa cggtggatgt gtcgcgatta cgccgtctgg
    18301 ttacgca
//
LOCUS       DAPOZO010000093        18250 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPOZO010000093 DAPOZO010000000
VERSION     DAPOZO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.093"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..666)
                     /locus_tag="ROI21_003612"
     CDS             complement(<1..666)
                     /locus_tag="ROI21_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDX5112489.1"
                     /translation="MQDIRQETLNECTRAEQSACVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTTDITKDKCSKCLSGCKFRNNV
                     GNFG"
     gene            complement(666..995)
                     /locus_tag="ROI21_003613"
     CDS             complement(666..995)
                     /locus_tag="ROI21_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5112490.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(992..3553)
                     /locus_tag="ROI21_003614"
     CDS             complement(992..3553)
                     /locus_tag="ROI21_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDX5112491.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDNAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAASGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            complement(3546..3980)
                     /locus_tag="ROI21_003615"
     CDS             complement(3546..3980)
                     /locus_tag="ROI21_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDX5112492.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(3962..4384)
                     /locus_tag="ROI21_003616"
     CDS             complement(3962..4384)
                     /locus_tag="ROI21_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDX5112493.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            complement(4400..5140)
                     /locus_tag="ROI21_003617"
     CDS             complement(4400..5140)
                     /locus_tag="ROI21_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5112494.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            complement(5148..5543)
                     /locus_tag="ROI21_003618"
     CDS             complement(5148..5543)
                     /locus_tag="ROI21_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5112495.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(5540..6118)
                     /locus_tag="ROI21_003619"
     CDS             complement(5540..6118)
                     /locus_tag="ROI21_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5112496.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(6130..6483)
                     /locus_tag="ROI21_003620"
     CDS             complement(6130..6483)
                     /locus_tag="ROI21_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HDX5112497.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATITSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(6495..6893)
                     /locus_tag="ROI21_003621"
     CDS             complement(6495..6893)
                     /locus_tag="ROI21_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDX5112498.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     AVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(6935..7960)
                     /locus_tag="ROI21_003622"
     CDS             complement(6935..7960)
                     /locus_tag="ROI21_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDX5112499.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(8015..8347)
                     /locus_tag="ROI21_003623"
     CDS             complement(8015..8347)
                     /locus_tag="ROI21_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDX5112500.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGSFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(8357..9676)
                     /locus_tag="ROI21_003624"
     CDS             complement(8357..9676)
                     /locus_tag="ROI21_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDX5112501.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLIAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLYVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQACVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(9657..11258)
                     /locus_tag="ROI21_003625"
     CDS             complement(9657..11258)
                     /locus_tag="ROI21_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDX5112502.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            complement(11255..11461)
                     /locus_tag="ROI21_003626"
     CDS             complement(11255..11461)
                     /locus_tag="ROI21_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDX5112503.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            complement(11458..12099)
                     /locus_tag="ROI21_003627"
     CDS             complement(11458..12099)
                     /locus_tag="ROI21_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDX5112504.1"
                     /translation="MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPT
                     IVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFT
                     LTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEAL
                     DCFVYALAALRISISRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            complement(12117..13384)
                     /locus_tag="ROI21_003628"
                     /pseudo
     CDS             complement(12117..13384)
                     /locus_tag="ROI21_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            complement(13359..13904)
                     /locus_tag="ROI21_003629"
     CDS             complement(13359..13904)
                     /locus_tag="ROI21_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDX5112505.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            14293..14526
                     /locus_tag="ROI21_003630"
     CDS             14293..14526
                     /locus_tag="ROI21_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDX5112506.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLCSEKRVSSEANKEKSDITELLRKQVRPD"
     gene            14584..14994
                     /locus_tag="ROI21_003631"
     CDS             14584..14994
                     /locus_tag="ROI21_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDX5112507.1"
                     /translation="MDQVVIFKQIFDKVRNDLNYQWFYSELKRHNVSHYIYYLATENV
                     HIVLKNDNTVLLKGLKNIVSVKFSKDRHLIETTSNKLKSREITFQEYRRNLAKAGVFR
                     WVTNIHEQKRYYYTFDNSLLFTESIQKTTQILPR"
     gene            complement(15146..15319)
                     /locus_tag="ROI21_003632"
     CDS             complement(15146..15319)
                     /locus_tag="ROI21_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDX5112508.1"
                     /translation="MNIENLKTKAEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDVKINLI"
     gene            complement(15491..15646)
                     /locus_tag="ROI21_003633"
     CDS             complement(15491..15646)
                     /locus_tag="ROI21_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112509.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            complement(15726..15791)
                     /locus_tag="ROI21_003634"
     CDS             complement(15726..15791)
                     /locus_tag="ROI21_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112510.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
     gene            complement(15794..15982)
                     /locus_tag="ROI21_003635"
     CDS             complement(15794..15982)
                     /locus_tag="ROI21_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDX5112511.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(15993..16205)
                     /gene="cspI"
                     /locus_tag="ROI21_003636"
     CDS             complement(15993..16205)
                     /gene="cspI"
                     /locus_tag="ROI21_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HDX5112512.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
     gene            complement(16568..17065)
                     /locus_tag="ROI21_003637"
     CDS             complement(16568..17065)
                     /locus_tag="ROI21_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HDX5112513.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
     gene            complement(17062..17595)
                     /locus_tag="ROI21_003638"
     CDS             complement(17062..17595)
                     /locus_tag="ROI21_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDX5112514.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            complement(17592..17903)
                     /locus_tag="ROI21_003639"
     CDS             complement(17592..17903)
                     /locus_tag="ROI21_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HDX5112515.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            complement(17908..18123)
                     /gene="essD"
                     /locus_tag="ROI21_003640"
     CDS             complement(17908..18123)
                     /gene="essD"
                     /locus_tag="ROI21_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDX5112516.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT         4131 a         5359 c         4328 g         4432 t
ORIGIN      
        1 gccaaagttg ccgacgttat tgcgaaactt acagccactc aggcatttgc tgcatttatc
       61 cttcgtgata tcggttgtcg gctggtcata ttcatccgcg acagccggac cgtgataacc
      121 gcactcatca ccgcgatagg tccaggtgca ggtgttggcc agcatgatac gtcccggaaa
      181 aacagcgcca tccgtttccg tcggcgtgga cagtacaaag gaggcactga ccgcgctcag
      241 ttcgctgcac tgctcgatgc gccagcggct gatcacctcc tgctccggat cggcgtcgct
      301 gtttccgttg acgaagttca ccgcatccag aaaacgggca taaaccttac gcctgaccac
      361 cgttccgccg accagactct gcaggtcttc cgccatcccg gtgaccatac cgtacaggtt
      421 agaaaccgtc agcgtggggc gcgtactggt acctttgcca ttcagttcga aaccactccc
      481 ctgaatggga tacgcctgat actgtcgccc ctgccaggtg accggctcac ctttttcgtt
      541 ctgctcatta cagaaaaaat aacgttctcc accaacctct gtcagatcga tttcccagag
      601 caccacgcag gcagactgct ccgcacgggt gcattcattc agtgtttcct gtcggatatc
      661 ctgcatcagt tcaccacctg ttcaaactct gcgctgaact caacacgcag catactgacc
      721 cgcgacgacc attttgcgca ggtcaccttt atctgccgcc actcataagg cggcgtccac
      781 agaaaggctt tccagccccc gtgctcttcc agaaacgact ccagtaccgt ggcctcctca
      841 cgggggacag aaagcgtcac gctgtacgtt ttcaggttgg cattcagccc ggcaggcgct
      901 cgctgggaat agccatcacc aaagcgcacc tttcttacag aaggggccga agccacatcc
      961 ataccgggtt tcactttcca gcggaaggtt ttcatcgtcc acctccggag aacaggccac
     1021 catcacgcat ctgtgtctga atttcatcac gggcaccctt gcgggccatg tcatacactg
     1081 ccttcatcat ctgtggacct ggcagaccat tcgtaccgtc gttctgaatc accacgtgat
     1141 tgttctgatt aaaattaatg ccttcagccc gccgcatctg cgccggactt ccggcaccgc
     1201 ccacataacc accttccgca tagccccgca tcaggcggta caggttgccg acaccaatcc
     1261 ggctggttgc ctccttcgtg aagacaaatt caccacggtg aacaatcccc gctggctcat
     1321 atttgccgcc ggttcccgta aatcccccgg tcgcaaaatg gaatttcgcc gcagcggcct
     1381 gaatggctgt accgcctgac gcggatgctc caccactggc agcaccgcca atggcgctgc
     1441 cgatactccc gacaatcccc accattgcct gcttaagcag aatttctgtc atcatggaca
     1501 gcacggaacg ggtgaagctg cgccagttct gctcactgcc ggtcagcatc gccgccatat
     1561 tctgtgcaat accatcaaag gtctgcgtgg ctgcactttt aacctgcgac atactgtccg
     1621 tggcgctctc ttcccactca ctccagccgg acttcaggcc tgccatccag ttcccgcgaa
     1681 gctggtcttc agccgcccag gtctttttct gctctgacat gacgttattc agcgccagcg
     1741 gattatcgcc atactgttcc ttcaggcgct gttccgtggc ttcccgttct gcctgccggt
     1801 cagtcagccc ccggcttttc gcatcaatgg cggcccgttt tgcccgttgc tgctgtgcga
     1861 atttatccgc ctgctgcgcc agcgcgttca ggcgctcctg atacgtaacc ttgtcgccaa
     1921 gtgcagccag ctggcgtttg tactccagcg tctcatcttt atgcgccagc agggatttct
     1981 cctgtgcaga cagctggcga cgttgcgccg cctcctccag taccgcgaac tgactctccg
     2041 ccttccacaa atcccggcgc tgctggctga ttttctcatt tgctccggca tgcttctcca
     2101 gcgtccggag ttcagcctga agcgtcagca gggcagcatg agcattgtct tcctgacgat
     2161 cgcccgcaga caccttcacg ccggactgtt tcggcttttt cagcgtcgct tcataatcct
     2221 ttttcgccgc cgccatcagc gtgttgtaat ctgcctgcag aattttcccg tccttcagtg
     2281 ccttattcag ttcttcctga cgggcggtat atttctccag cggcgtctgc agccgttcgt
     2341 aagccttctg cgcctcttcg gtatatttca gccgtgacgc ttcggtatcg ctctgctgct
     2401 gcgcattttt gtcctgttga gtctgctgct cagccttctt tcgggcggct tcaagcgcaa
     2461 gacgggcctt ttcacgatca tcccagtaac gcgcccgcgc ttcatcgtta acaaaataat
     2521 catccttgcg cagattccag atgtcgtctg ctttcttaaa cgcagcctct gccttaatca
     2581 gcatctcctg cgcggtatca ggacgaccaa tatccagcac cgcatcccac atggatttga
     2641 atgcccgtgc agtcctgtct gcccaggtct ccagcgtgcc catgttctct ttcaggcggc
     2701 gggtctggtc atcaaaccct ttcgttgcgg cctcgttcgc tgcctgcaat gccccggcct
     2761 catcgccgga acgctgcaac tgagcaacat acgcaatctg ctccgccgtc acgttatgga
     2821 actggcgcgc catcgccgtc agccccgacg tcgggtctgt ggtcagcttc ccgaaggctt
     2881 cagcgacctt gtccacctcc acgccggatg cagaggagaa acgcgccaca ctctggctga
     2941 tggacgcaat ctgagcctca ccgcttactc ccgccttaac cagtgcgctg agtgactcgc
     3001 tggtctggtt aaacgtcagc cctgccgcct gcccggctct ggacaggacc agcatacgat
     3061 ctgccgtcag acccgactga ttgccggaaa ggaccagcgt tttgttgaaa tcggacaggg
     3121 ttgagttgcc ctgataccag gcatacgcca gcgcaccggt cgccaccgcc agcgaggtgg
     3181 ccccgaccat cggcagggtg atcgcaccgg caagcccccg gaacatgggg atcatcccgc
     3241 cgaaggagtc cttcacctgc cccccctgtt gcagcaggat cagccacgga ctttgcccgc
     3301 ctgcaagctg cgtggccacg tcggtgaact gtgcaggcag catacgcatg gcggctttat
     3361 actgcccgac ggaaatcccc gctttctgtg cagccagcgc ctgtcggctc agcgactgtt
     3421 caacgactgc cgctgttttt ttcgcatcac tttccgtacc ggaaaaatga cgcctgactc
     3481 tggccatctg ctcgtcaaat ctggccgcat ccagacttaa atcaacgacc agatcgccta
     3541 ccggttcagc cataccggac tcctcctgcg atcccttctg atactgtcat cagcattacg
     3601 tcatcctccg tcatgtccgc cacatccggg gaagcgggga taacttcatt cccgtccggg
     3661 ccaaagcgga cacctccggc aagccctgcc gctttctgca tcagcacatc atcttcaggc
     3721 tcttcgtcag cctcacgccg gttcagcaga ctgaaatcca gcggatgcat atccggatcg
     3781 ctgaaaaaca ggctgagcac ggtgtacgtc agcccggaaa agtgcatatc cagcagaaca
     3841 tcatgaaaat aatgggtact gtaaaagcgg tgccagtcgg catactccgt ggatgacatc
     3901 ccggcaagca tggcacgcca gtcgggtcgc cccatctcac gcgccagttt cagggcaaaa
     3961 ctcagctcac cgtcgaacac tttcccgcag aaacaggctc tgcgggcccg gcgtcctctg
     4021 tctgttcagg ggcattattc accacaaact catacatacc agacagccgg tacaccacgt
     4081 tttcagcatg agaaattgcc tccgtgggcc aggtggtaag cacttcctgc tcaatctgtt
     4141 taacggcttc attcatggac ggcatctgcg tcttctgcgg atggttatgc cacagggaca
     4201 tcgccaccag aaacgcgccg gttctgatgg cgtcttccac agtaaacttc cggttgctgt
     4261 ctgactccgc ctgttctgcc tgccgtttca tcagggcgag atgctcaatg cgctgcaggg
     4321 ctgacagttc agaaagcgtg acggtcacac cgttatgttc aaatgattcg gttttcagga
     4381 acatcgctga ctctccggat taactggcgg tgacggtaat ttctgcaacc gcagcaaact
     4441 caccattacc ggatacaacc ggaatgttga ccttgcctgc agcaacgccg ttcacggtga
     4501 tggtcatacc actgaccgac acggtggctt ttgttttatc cgcagacacc gcacgaaagc
     4561 tcttgtcggt tacgccctcc ggctggaagg ccacggtcag cgtggtgctc tgccctttca
     4621 ccaccgaggt gctggcaggc gtcacggtca tgccggttgc cgctgttacc gtgctgcgat
     4681 cttctgccat cgacggacgt cccacattgg tgactttcac cgtgcgggtg atcacttcct
     4741 tcgccgtcac cgccttaccg atactgctga cccagccacg gaacacatcg accgtgccgt
     4801 tcgggaagcg gattttatag gcacgggtat cgccttcatt aaaccacgcc agcagcgcct
     4861 gctgcccctg ctctccgggc atccacgcca gcgtgaagct ggtatctccg gcagatttct
     4921 gcccctgccc ggtcgcagtc cagtctgcat cttcatcatc gagatagctg tcgtcatagg
     4981 actcagcggt cagttcgccg ggcgtcaggt ctttaacttt tgccagacgc gaccagtcaa
     5041 cgtctgaaag cggattcgca taagggtcac cgctcccctt ataaacccac agtgtggtcc
     5101 cggcaccttt caccggcatt gttggatttg gtacaggcat agcgtcctca catttcatag
     5161 gtaatgacat aagtcagatc ggctgaactc cacaggcccg catcatcgtc gcgccggtag
     5221 tcatagccac tggccaccat actggtgatc aaatctgaca gtgccgggat atcgctcatc
     5281 accggataaa tccgggactc catccacgaa tccagctctg aatccggcac ctgagcaggc
     5341 aggaaaactt caatatgcag ctccgcctgc caggtatcgc tgtccagctc ttcgcccgtg
     5401 tattcagcgc cggtgagata aacggcaatt gccggaaaat ccgcctcatc aaaaacagcg
     5461 gggcgaccat caaaaagcgt cgccccggtg tcatgcttct ccagtgcatc cagtacggct
     5521 gcacggagtt cagtatgttt catcgcttta ttaccattct cagttgatgc tgcagcgcat
     5581 agcccagctc tttcggaaga cgttcacgcc gtatccgttc aatattctgt ttaaacgccg
     5641 tggtaagcgg caccgccatc gggattttca ccacatcaat ggggtaacgg tttttcccgg
     5701 ccacacgctg catgacatgc caccgcccat ttttcagttg ctgaataaac gcgccgggaa
     5761 tacgacggtt acccaccaca agcacgctgc cgccaccttt cagggatgaa cgctgcccct
     5821 ttttacgacg cctgcggcgg gacaggacaa cccgcgcatt acccagcttg attacgggca
     5881 aatccccccg gttaactttg attctggcct gcgggttttt gaccgtggcc cttttcagcc
     5941 tggccctttc ctttaccagt ttccggcgta cctttgtctc acgggcaacc tgtgacgccg
     6001 actgcgatat cgcggatgaa gcaacgcggt taatggccat tgcggcggca ccgggcaccg
     6061 ccgttttgct gatacggctg aggttttcaa cggcctgctc aagacctttt atggccatac
     6121 atcccccttt cagcggcgac ggttaacggc aggcggcacg ccccgtccaa gccagagatg
     6181 acagcttccg ccatcatccg gcgaaacccg atctacccag aaattttcct caccgatggt
     6241 cagcgtgtct ccacgccgca gctgccgcac ctcatcagtc cggacaaaca gggacgggct
     6301 ggagccttca acgcgcacgc cctgtccggc atagctgata ttttcagggt catcaaaaac
     6361 accacgtatc accgcaccgg actgctcacc ggatgtaatg gtggctgacg ttcccatgta
     6421 cccgcgtatc gtttcatcgg cgcgggcaat ggcagcatcg aacaggttat cgaaatcagc
     6481 cacagcgcct cccgttattg cattctggcc aggccgcgct ctgtcatttc ggctgccaca
     6541 ccggcagaga cacgaaacgc cgttcccggc agcacaaatg ccacagcctc atcccgcgtg
     6601 gcgtgaagtg catcagtatg cagcttcacc agtgccacga ccgtgaccag ttcagacgta
     6661 tccagaatca cggtatccgg ctgcgctgat cccacctcat tttcatgtcc ggtcagcaca
     6721 ttttcccggc tgagaggggt gtcctgaccg gcagtttcat ccgtgtcatc aagctcctct
     6781 tccagctctg ccacacggag cgccagttct tctttcgtcc ccgtcaggct gacatcacgg
     6841 ttcagttgtt cacccagcga gcggagacgg gcaatcagtt catctttcgt catggactcc
     6901 tccacagaga aacaatggcc ccgaagggcc atgattacgc cagttgtacg gacacgaact
     6961 catcagggtc agccagcagc atcagcggtg ctgactgaat catggtgaac tcacgcgccg
     7021 gatcgccggt ggtcacccag tttttcgggt aacgggcaga ggcgttaatg ccttcgcgct
     7081 gtgcgtccgc atcctgaatg cagccatagg tgcgcagacc gcgtgcctga gtgttcccca
     7141 gcaccatcgt gttgtccggc aggaagttct ttttgacgcc gttttccacg tactgtccgg
     7201 aatacacgac gatggccaca tcgccataca tccccttata ggacaccgct ttacctaggt
     7261 ctttcaccgc tgtctccagc tcggaatgag agccgcgacg agtatccagc ttctccttga
     7321 cggctttgaa ggaacggaac agcgcccagc ctttcggatc gaacacgatg atattcacca
     7381 caccgctggc gttcagcgcg taggcttcga tatcgtcggt cgggtcatac gtggacttgt
     7441 cacgcttgct ccactccgtg ccgccggact gcgtgatgtt attctcctca ctgcggccca
     7501 tatccacctc aaccggatcg aaggcttcac cggtcatggt gtatttgccc ttaagcacgg
     7561 cagaaactgc ctgcatctct tcgacctgag caatggccag ctcttcgtca cgcatgttct
     7621 gcatgatgat gcgacggcgg cggtaagccg ggtccgccag attctgcgga tcttcatccg
     7681 gcaggcgacg cagggtcatc tgcggattca cttcatgctt cggcttgaca tatcccggcg
     7741 taaattcaga ggtggagccg ccacgggaac ggataacctc accggaaaca atcggcgaaa
     7801 cgtacagcgc catgtttacc agtcccggaa tttgtgagag atagactttc tccgtagtga
     7861 agggatagct ctcacggaaa aagagacgca gaaacagcgg atcaaactta aatttctgct
     7921 catttgccgc cagcagctgg gcggttgtgt acatcgacat aaaaaaatcc cgtaaaaaag
     7981 ccgcacaggc ggcctttagt gatgaagggt aaagttaaac gatgctgatt gccgttccgg
     8041 caaacgcggt ccgttttttc gtctcgtcgc tggcagcctc cggccagagc acatcctcat
     8101 aacggaacga gccggacttg tagaacgtca gtgtggtgct ggtctggtca gcagcaactg
     8161 caagaatgcc aacggcagca ccgtcggtgg tgccatccca cgcaaccagc ttacgggtgg
     8221 aggtgtccag catcagcggg gtcattgcag gcgctttcgc actcaatccg ccgggcgcgg
     8281 ttgcggtatg agccgggtca ctgttgccca gcggctggta atgggtaaag gtttctttgc
     8341 tcgtcataaa catcccttac actggtgtgt tcagcaaatc gttaacggca tcagatgccg
     8401 ggttacctgc agccagcggt gccggtgccc cctgcatcag acgatccagc gcagtgtcac
     8461 tgcgcgtctg tgcactctgt ggtgctgcgg ccagaatacg gcgggccgtt tccacggtca
     8521 taccaggggt ttcggccagc acgcatgcct gttcttcgcg tccgtgagcc tcctcacagt
     8581 tgaggatccc cataatgcga ctgttttctg ccgcaaccgc tgcggtgatc tgcgcgttca
     8641 cgtccggctg cgcagcgctg gcgttctcgc cctccgtcgc ttgcaccacg ccagtaacgt
     8701 cagcctgcga agcagtggct gaaacagttg ttgattgagt ctctttggtc attcgccctc
     8761 ctgagagacg ggatttacgt gcatccagtg catcacgcat gacggtgatc gcatcggtac
     8821 tgttcacaag ttcatcagcc agtccggcat caatggcctc ctgaccgctg tacactgcag
     8881 cctcggtatc cagcacagcc tgcacgtaca ggccggtata tgccgacacc ttctgtgcaa
     8941 acatccggcg ggttgcatcc atccgggact gcagtgtttc ccggacatca tccggtagat
     9001 ggctgtaggg gttgccatcc accttatggc tgccgctgta aatcagcgtg atttccacgc
     9061 cctgtttctc cagcgcagca ccgtaattac tgtgagccat catgacgccg atggagcctg
     9121 tccgggcggt ctgcgtgacc agacgccggg aggcggcgct ggcaagcagc tgacctgcac
     9181 tgcagttcat gtcgttggcc agcgcccata ccggttttat gtctcgcaca cgggcgatga
     9241 tgtcagcaca gtcaaatgct cccgccacca tcccgcccgg tgtgtccata tccagcagaa
     9301 tgccgtccac catcggatcg ctggcagcct gttgcagacg ggcgataatg ccgttgtaac
     9361 cggtcattcc cgaatacggc tgcagcgccc gcgtccggct gaccagcgta ccggacaccg
     9421 gcagcacggc gatgccgttc atgacctgat aactgcgggc ctgtcgtggt ccgtcatcat
     9481 caccggataa cgccagcgcc gcgggtgcct ctccggcaat caggctgtcg ccggatactg
     9541 catccgtcag gcggctgatc ccaagctggc ctgcaagcgc acaaaagaaa acccgcgcat
     9601 aggcgggttc aagcatcagc ggctcattaa aagccatgct ggcaatatgc gggagattac
     9661 gcagctctgc tgtcactctt ctcctcctct gttgattgtc gcagtccgga ttcaaatgcc
     9721 gcagccgccc aggcgggcgg tttaagaccg gctgcacggc gctccatcgt ttcacggacc
     9781 tgctgggcaa aaatttcctg atagtcgtca ccgcgtttcg cgcactcttt ctcgtaggtg
     9841 ctcagtccgg cttctatcag catcaccgct tcctgaactt ctttcagacc atcgatggcc
     9901 atacgaccgg agcctatcca gtcgcagttc ccccaggcac tgcgggcttc ctgaaagctg
     9961 aagcgcgctt ttgaaggtaa cgtcaccacg cggcgaacga tggcctcttc cagccagcac
    10021 aaaaacatct ggctcgcctg acgggatgcg acgaattttc gccgccccat aaagtacgcc
    10081 cacgactcgt tcgcactggc ccgtgccgtg gagtagctca tctgggcgta attccgggaa
    10141 agctgctcat acgagacacc cagcccggca gcgatatacc gcaacagtga ctgctcaaac
    10201 acggagtagc cgttatccgt gtcctgagcc gtctgcaggt tcagtgagtc ccccggcatc
    10261 aggtgcggca cttttgcgcc tcccagacgg accggtgctg cagcgtaata cgcggcaatt
    10321 tcaccaatcc agcccgtcag cctttcccgc tgctcctgac tgttcgcgcc cagaataaaa
    10381 tccatcgctg actgcgtatc cagctcactt tcaatggtgg cggcatacat cgccttcaca
    10441 atggcgctct gcagctgcgt gttctgcagc gtgtcgagca tcttcatctg ctccattacg
    10501 ctgtaaaaca catttgcacc gcgggtctgc ccgtcctcca cgggttcaaa aacgtgaatg
    10561 aacgaggcgc gcccgccggg taattcacgg ggtatccatg tccatttctg cggcatccag
    10621 ccaggatacc cgtcctcgct gacgtaatat cccagcgccg caccgctgtc attaatctgc
    10681 acaccggcac ggcagttccg gctgtcgccg gtattgttcg ggttgctgat gcgcttcggg
    10741 ctgaccatcc ggaactgtgt ccggaaaagc cgcgacggac tggtatccca ggtggcctga
    10801 acgaacagtt caccgttaaa ggcgtgcatg gccacacctt cccgaatcat catggtaaac
    10861 gtgcgttttc gctcaacgtc aatgcagcag cagtcatcct cggcaaactc tttccatgcc
    10921 gcttcaacct cgcgggaaaa ggcacgggct tcttcctccc cgatgcccag atagcgccag
    10981 cttgggcgat gactgagccg gaaaaaagac ccgacgatat gatcctgatg caactggatg
    11041 gcgttggcgg catagccgtt attgcgtacc agatcgtctg cgcgggcatt gccacgggta
    11101 aagttgggca gcagggctgc atccacactt tcacccggtg ggttccacgc ccgcaactgc
    11161 ccaccaaatc cgctgccacc gccgtgataa ccggcatatt cacgcagcga tgtcatgccg
    11221 tccggcccca gaagggtggg aatggtggac gttttcatac ataaaatcct gcaggtcccc
    11281 tgcgtcgctg tgtcatgccg gtctgcactt ccagctccgc aatgtatttt ttcaggtcag
    11341 acacggaagt ggccgtaaac tccactcgcc gtccgtcttt ctgtaccgtt gccacccgtt
    11401 ttcctgtcat caggtcatgc agtgccgcac gggcagcggc aagttcttcc tgtcgcgtca
    11461 ttcatcctct ccggataagg cacgggcgta atctgccagt gttttcttgt tggttgctgc
    11521 accatcctct tcctgcaggc tcgccagcag tgcactgaga tccagctgcc agcgggaaat
    11581 actgatgcgc agcgccgcca gcgcataaac gaagcagtcg agcgcctcat tgcgtcgctt
    11641 tttgctgtcc cacagtattt ttttcctgcc atccacccat ttttcgacct gctcttcagc
    11701 agtcagctgc tgcgcttcgg tcagatcaaa aatatccggg ttattcggga agtgaacggc
    11761 accgggaagc ggttcatccc cttccggcgt cagtgtgaag cggttataaa tctgctcttt
    11821 cgcggtatcc gtaccgattt cggtaaggta aaccccgttt ttgtttcgct tacgtggcat
    11881 gctggccact ggctttccgt agacggatgc ccctttaatg gggatcaccc ggaacagccc
    11941 atgctttttc gagcgttcat acacaatggt cgggtcaatc ccgccagtat cccagcagat
    12001 acgggatacc gacatttctg caccattccg gcgggtatag gttttattga tggcctcatc
    12061 cacacgcagc agcgtctgtt catcgtcgtg gcggcccata ataatctgcc ggtcaatcag
    12121 ccagctttcc tcacccggcc ccccatcccc atacgcgcat ttcgtagcgg tccagctggg
    12181 agtcgatacc ggcggtcagg taagccacac ggtcaggaac gggcgctgaa taatgctctt
    12241 tccgctccgc catcacctca gcatccggac gttcgccaat tttcgcttcc catgtctcac
    12301 cgagcgtggt gttcacgaag gttttacgtt ttcccgtatc ccctttcgtc ttcatccagt
    12361 ctttgacaat ctgcacccag gtggtgaacg ggctgtacgc cgtccagatg tgaaaggtca
    12421 cactgtccgg cggctcaatc tcttcaccgg atgacgaaaa ccagagaatg ccatcacggg
    12481 tccagatccc ggtcttttcg cagatataac gggcatcagt aaagtccagc tcctgctggc
    12541 ggatgacgca ggcattatgc tcgcagagat aaaacacgct ggaggggtca tccggcgtcc
    12601 atttgaggcc aaacggcgtc tctttgtcgc caaatttaag atactgctcc tccccgcagt
    12661 gcgggcaggc aacatgaaaa cgcataaaat gcggggattc actggctgca cgctcaatct
    12721 ggcaggtgcc tctcactttg ggcgtggagc cacggatgga ctttggccag accgagcctt
    12781 caatacgttt gtcgccaagg aacgtcggag agccttcctg ttcaatatcc tcatcaaagg
    12841 cagcaagttc atcataaccc gccacatcca ccgacttttc acggtagttt tttgccgctt
    12901 taccgcccag gcaccagaag ccacgaccat tggaaaaacg cttcatagtg agcgtgttat
    12961 cccggtgctt tttgccatac cacggagcca gcgccagcag cgacggaata tcgcggatgg
    13021 tcggctcaac gtgggttttc ataaagttct cggcatcacc atccgtcggc aaccagataa
    13081 gggtgttgcg ctgcttatgc tctatgaagt aggcataaac acccagcagc attttggaat
    13141 aaccaacacg ggcagacttc accacattca cctcgcggat gtagtcactg cccatcgcat
    13201 tcatgatggc ccgctgaaag ggcagtgttt cccagcgccc ttcctggtat gcggattctt
    13261 tcgggagata gtaattggca tccgcccatt caacggcggt ctgtggctcc ggcctgaaca
    13321 gtgagcgaag cccggcgcgg acaaaatgcc gcagcctgtt aacctgactg ttcgatatat
    13381 tcactcagca accccggtat cagttcatcc agcgcggctg ctttgttcat ggctttgatg
    13441 atatcccgtt tcaggaaatc aacatgtcgg ttttccagtt ccggaaaacg ccgctgcacc
    13501 gacaggggga tcccgtcgag aatactggca atttcacctg cgatccgcga cagcacgaaa
    13561 gtacagaatg cggtttccac cacttcagcg gagtctctgg catttttcag ctcctgtgcg
    13621 tcggcctgtg cacgcgtaag tcggtggcgt tcgtactcaa tagtccctgg ctggagatct
    13681 gtctcgctgg cctgccgcag ttcttcaact tcccggcgca gcttttcgtt ctcaatttca
    13741 gcatcccttt cggcatacca ttttataacg gcggcagagt cataaagcac ctcattaccc
    13801 ttgccaccgc ctcgcagaac gggcattccc tgttcctgcc agttctgaat ggtacggata
    13861 ctcgcaccga aaatgtcagc cagctgcttt ttgttgactt ccattgttca ttccacggcc
    13921 aaaaacagag aaaggaaacg acagaggccc aaaagttcgt tttcagcacc tgtcgtttcc
    13981 tttcttttca gggggtattt taaataaaaa cattaagtta cgacgaagaa gaacggaaac
    14041 gccttaaacc ggaaaatttt cataaatagc gaaaacccgc gaggtcgccg ccccgtaacc
    14101 tgtcggatca ccggaaagga cccgtaaagt gataatgatt atcatctaca tatcacaacg
    14161 tgcgtggagg ccatcaaacc acgtcaaata atcaattatg acgcaggtat cgtattaatt
    14221 gatctgcatc aacttaacgt aaaaacaact tcagacaata caaatcagcg acactgaata
    14281 cggggcaacc tcatgtcaac gaagaacaga acccgcagaa caacaacccg caacatccgc
    14341 tttcctaacc aaatgattga acaaattaac atcgctcttg agcaaaaagg gtccgggaat
    14401 ttctcagcct gggtcattga agcctgccgc cggagactgt gctcagaaaa aagagtttct
    14461 tctgaagcaa acaaagaaaa gagtgacatt actgaattgc tcagaaaaca ggtcagacca
    14521 gattgaagca atttagataa tcgtgcagac tacgccccct catatcacat ggaaggttta
    14581 tctatggatc aggtagtcat ttttaaacaa atatttgata aagttcgaaa cgatttaaac
    14641 tatcaatggt tttattctga gctaaaacgt cacaatgtct cacattacat ttactattta
    14701 gccacagaga atgttcatat tgtattaaaa aatgataata cagtgttatt aaagggccta
    14761 aaaaacattg tgtctgtcaa attttcaaag gataggcatc ttatagaaac gacctctaat
    14821 aagctgaaat ccagagagat cacatttcag gaatacagaa gaaaccttgc taaagcagga
    14881 gtttttcggt gggttacaaa tatccacgaa caaaaaagat attactatac ctttgataat
    14941 tcattactat ttactgaaag catccagaaa actacacaga tcttaccacg ctaaaccata
    15001 acgtccggct tctctcactc ctgagccgga ctgcattggt ttaataaaaa ccatcaacaa
    15061 ttgtgattta gatattcgga accattcaaa tataacaaaa ccccgtaaaa acgaggttta
    15121 tggataaatt ttattattga atacatcaga ttaaattaat cttgacatca tagctttcaa
    15181 gacccgtcat tttttcccgt gcggtaaact gaatactggt aacttctttc ccggtctttt
    15241 tcttaagttc aataattttt tttgttatat attcagaaat atctgcttct gcttttgttt
    15301 ttaagttttc aatattcatc atttcctctt ttagtctgtt atgactttcc agttacacag
    15361 taagtcgatt atatggtgca aacgtgtaaa agataagatg aaacatcgca ataatcaaca
    15421 tacgatagtc taaattttac acaaacagac aaagagaatt ttcctgaatt atcaatgcaa
    15481 tagcatcaaa tcaactcaag agccttattg ctgcttccag aatttcttct gaagtaacat
    15541 gtcgatccgc ggctacataa atgactttat gatctccggt cagagatgga aaccctgcgg
    15601 ccattacagt aaggtgtgtt ttttcgccat ttggatattc acgcatgatg gtgttaactc
    15661 cagtcatcgc tggcactacc actgctggtt cagagttaaa aaaaactatg atttttttca
    15721 tgatgttacc gtagtatgtg agtatccatc gaatagacac caagcaaaaa agctcccgaa
    15781 ggagccttca ttttcacttt tttaaatcca acgacagacg gctggcattt aagtattgtg
    15841 aaatattatc aaatgtaatc atcattgatt tacaaaagat acattttgcc ccgaaaggat
    15901 tcatgtcaga aacatcaaaa gatgatgttc tatactggga accatgacaa cacgggcatc
    15961 taaagtgaat atggtttgta atattgtcta cctcaaagcg ccactacatg aacagcggca
    16021 ggacctttag gtccgttctc aataccaaat tcaacttcct gattctcagt taatgttttg
    16081 aaatcgttgc tctgaattgc tgagaaatgg acaaacacat ctttgctgcc atctttcggc
    16141 gtgatgaaac caaaaccttt ttcagggtta aaccatttca ctaaaccagt cattttgtta
    16201 gacataatta ttaccttttg aagaaattag cccttgggca gaatggtccg aaaaaaaata
    16261 tcagagagaa aaaccaacaa ggaaatctca agaggtacaa ataataaaat tataacaatg
    16321 actgcttcag ataaatttgt aacaaaccag aacaccatta acgcatgatt aaccacccat
    16381 agcaaggatt acttttgtaa agaaaaacac agcaatgaaa gaatagcttt atttattaat
    16441 aaaacgtgtc attctgatta agacctttta tcttaccctt aagatttcag gaattttggc
    16501 tcatggaaga gtccttttta tttaaatttt acattccgcg atgtaaatgt tccgatttaa
    16561 tattacccta catttgatgc tttttatctc ttaaagattc atagatctgt tgacaagtca
    16621 ctcctgcgat gtagcgttcg tcagcaattt cagcataaag ctgagcttct gctgcaatat
    16681 ctccgagcat gttggtgagc attccttcgg cggttttggt tgttttgcct ctgacggcag
    16741 cggcaagatc tgcggtatgc ttcgctgcgt caaggcgtat ggcatatttt tttgcttcgg
    16801 cacgcaactg gttaacacta tcagacagat aagcagccct ggcagaaatt tcagcagatt
    16861 tctgttgcgc atctttaaca gcctcatcac gggctatagt tcgcccctgt tcaattattc
    16921 gagcagcaaa ttgagcattt acctcttgtg ataatgcggc agcatcacgt tccgcccatt
    16981 ttttttgcca tcctcggtcg ctccagacat ttccgacgat aaatcctgac aacacgagaa
    17041 aaatcaccat gaatatctga ttcactgttc tatcccccag caggttaatg cgctctcctg
    17101 gtcacgacga ataacctgac cgtaacagtt atttgaacga atgcggcaat cgcgtccgcc
    17161 atccttaatc caccagcgaa tcgcttcgca tgcaccttta cgatcaccag cattcagccg
    17221 cttataaaac gtcgacggga aacacttacc ggggccaatg ttatagggac aaaatgacgc
    17281 gatacccgct ttttgtggtt cggtcagtgg tactttaata ttgcgctcca cccatgccag
    17341 cgccttatca cgctcaatgg cgttgacctg gtcgcatttt tccttcgaca gtttcatatt
    17401 gggaaaaacg gtttttccat ccaccactgt ggcaccccga cagatggtcc atatgccaga
    17461 accatcgcgg tatgccattg tgtggttacc ttctttttcg tccagaaact ggtcaagtat
    17521 ctgaggagca gatgcgccag caccaatcag cgccagaacg gcagccgaca ggccgtatct
    17581 gatttttgtg ttcatagata tttatgatga ggacgctcgt gcttattggc aggattttca
    17641 atcttaaagg agtactgatg ctgcagataa gactcaactt tttctgacaa tttttctgct
    17701 acttccagga agacttgccg gacgctcctt ctggctgctg cctcataaaa ctccagcgca
    17761 gctccttcaa cacggtccat ggcgacatcc aggtcaaaaa tttcaccgtc aaagcgttct
    17821 ttgtcctgta aggctacagt taccgtaact ttattctcaa aattacggac tcctttcaca
    17881 accagttcat agtcttgagt cattggatta ctctcctctc gcagccttac gcctgtcttc
    17941 tttaatcttg aaataaagat ttgtcagata cgtcagcagg ccaaaaacca ggctacccag
    18001 cacaccgatt gcagcccact gtgacggagt tactttatcg agtaactgca atgcccagaa
    18061 accagcatta cccgccgatg tgccataggc aacacctgtt gttaacttat ccattgattt
    18121 catatcctca ccccgatgta cacggatggt gcaatatgtt tgaaaagatc ggagtctacg
    18181 gggtagtttt gacagcacac gttgttctca acggcgctaa aaaaacatac acattaaaaa
    18241 tgtgggtaat
//
LOCUS       DAPOZO010000094        17403 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPOZO010000094 DAPOZO010000000
VERSION     DAPOZO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.094"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..452)
                     /locus_tag="ROI21_003641"
     CDS             complement(<1..452)
                     /locus_tag="ROI21_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDX5112517.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAAR"
     gene            complement(499..1218)
                     /gene="yehT"
                     /locus_tag="ROI21_003642"
     CDS             complement(499..1218)
                     /gene="yehT"
                     /locus_tag="ROI21_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDX5112518.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1215..2900)
                     /gene="btsS"
                     /locus_tag="ROI21_003643"
     CDS             complement(1215..2900)
                     /gene="btsS"
                     /locus_tag="ROI21_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDX5112519.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDCYTRITLRLPWRDEA"
     gene            3122..3853
                     /gene="mlrA"
                     /locus_tag="ROI21_003644"
     CDS             3122..3853
                     /gene="mlrA"
                     /locus_tag="ROI21_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDX5112520.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            3913..4020
                     /locus_tag="ROI21_003645"
     CDS             3913..4020
                     /locus_tag="ROI21_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDX5112521.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(4001..4732)
                     /gene="yehW"
                     /locus_tag="ROI21_003646"
     CDS             complement(4001..4732)
                     /gene="yehW"
                     /locus_tag="ROI21_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDX5112522.1"
                     /translation="MKMLRDPLFWLIALFVALLFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDVSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(4737..5663)
                     /gene="yehX"
                     /locus_tag="ROI21_003647"
     CDS             complement(4737..5663)
                     /gene="yehX"
                     /locus_tag="ROI21_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDX5112523.1"
                     /translation="MIEFSHVSKLFGIQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(5656..6813)
                     /gene="yehY"
                     /locus_tag="ROI21_003648"
     CDS             complement(5656..6813)
                     /gene="yehY"
                     /locus_tag="ROI21_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDX5112524.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWMTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGALNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGVWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(6820..7737)
                     /gene="osmF"
                     /locus_tag="ROI21_003649"
     CDS             complement(6820..7737)
                     /gene="osmF"
                     /locus_tag="ROI21_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDX5112525.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(7886..10183)
                     /gene="bglX"
                     /locus_tag="ROI21_003650"
     CDS             complement(7886..10183)
                     /gene="bglX"
                     /locus_tag="ROI21_003650"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDX5112526.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLEAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            10379..12094
                     /gene="dld"
                     /locus_tag="ROI21_003651"
     CDS             10379..12094
                     /gene="dld"
                     /locus_tag="ROI21_003651"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDX5112527.1"
                     /translation="MSSMTTTDNKAFLNELARLVGSSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12133..13065)
                     /gene="pbpG"
                     /locus_tag="ROI21_003652"
     CDS             complement(12133..13065)
                     /gene="pbpG"
                     /locus_tag="ROI21_003652"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDX5112528.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13239..13826)
                     /locus_tag="ROI21_003653"
     CDS             complement(13239..13826)
                     /locus_tag="ROI21_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDX5112529.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            13996..14574
                     /locus_tag="ROI21_003654"
     CDS             13996..14574
                     /locus_tag="ROI21_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDX5112530.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(14704..15465)
                     /locus_tag="ROI21_003655"
     CDS             complement(14704..15465)
                     /locus_tag="ROI21_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX5112531.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGALALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDGDVKPGAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(15518..16954)
                     /gene="mdtQ"
                     /locus_tag="ROI21_003656"
     CDS             complement(15518..16954)
                     /gene="mdtQ"
                     /locus_tag="ROI21_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDX5112532.1"
                     /translation="MNRDFFYPAIACFPLLLMLTGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQSAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVAQAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGLIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17178..17261
                     /gene="yohP"
                     /locus_tag="ROI21_003657"
     CDS             17178..17261
                     /gene="yohP"
                     /locus_tag="ROI21_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDX5112533.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         4219 a         4836 c         4324 g         4024 t
ORIGIN      
        1 tggcgcgctg ccagtccacg cagaaaataa cgtaaaaatt gatcgccgca ttcgcggaag
       61 tttttatgat ccggtgcacg catcatcgcc gtaatttccg gcatcgaaac gcggaactgc
      121 tgttcggtaa ggatcgccag aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt
      181 ttcagcacga tgttgttatt aatgcgacgt tccggctcca gtgccggagc agactcatcc
      241 ttgccgcgtt tttcataaat caggccattg aggaaggaag acaaaacaat gtccggacaa
      301 cgctgaaaac cctcttcgtc ttctttacgt agccagacgg cgatctgttc cgcggtggct
      361 tcgacattac ccagcgccag aatacgcacc aggtcattat tattggcttt caaaatgtag
      421 cgcacgctgc gcagaatatc gttacttagc atgaggcctt caggtgttga tgaggcaaaa
      481 agccatttta gcagtctttt aaaggccaat cgcctctttt aagcttttca gataacggcg
      541 gctgaccggc acggttaagc cattacgcaa aatcaactcg gcctggccgt tatcttccag
      601 acgaatctcc tgtaaatgcg cgaggttaac cagatactga cgatggcagc gcagtagtgg
      661 tgtacgactt tccagggtac gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct
      721 ggtaacgtag acaccgctca tccgactgct gacaaatgcc acatctttca tttgcagcaa
      781 ataaatccga ctatgccccg tacaagggat aaacttcagc gcctgttgat tttccggtaa
      841 cagcgaaaca tcctgcttgc tgcgctcctg acgcaatcgc gccagcgttt tttccagtcg
      901 cgcttcatca attggcttca gcagataatc aaaggcatgt tcttcaaagg ctttaatagc
      961 gtattcgtca aacgcggtga gaaaaacgat atacggtcga tgttcggggt cgagcatccc
     1021 caccatttcc agaccactga tgcgcggcat ctggatatcg agaaacagca catccgggcg
     1081 cagtttatgc accgcgccga tcccttctac ggcgtttgaa cactctccaa cgatttcaat
     1141 atcgctctgc tcctgcaaaa atacgcgcag gttctcccgt gcaagcggtt catcatcgac
     1201 aattaagact ttaatcatgc ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa
     1261 caatcaggct cacaggcgac gcttatccca tagtcatcgc caaaccgttc acgtaaacgc
     1321 ttatccacca gattcatccc cagcccactg gcattggtta ccggttgata caaaccggca
     1381 ttgtcttcga tctccagcat caaatgttgc ccctcacgtc gggcgctgat cgccactcgc
     1441 cctgtatcca gcagttgtga tgtcccatgt ttaatggcgt tttccactat cggttgcagg
     1501 gtaaacgcgg gcaattgctg ctgggataat tcttgcggaa tagcaatatt gacctgcaac
     1561 cgcgactgga agcgcgcctt ttcaatttgc agataagcgt tcacatgttc aatttcgtcg
     1621 gcgagagtaa caaactccga aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac
     1681 tgcaccagct ggctggcctg ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc
     1741 gcattaaaca aaaaatgggg gttcacctgg gcgtgaagca gtttgatctc tgactgggtg
     1801 agcatcgctt tttgccgctc atattgcccg gcgaggatct gcgccgaaag cagttgcgca
     1861 atcccctcac ccagcgtgcg attgattgaa ctgaataaac ggtttttggc ttcatacaat
     1921 ttgatggtac ccatcacccg ctgattttca ccacgcaacg gaattaccag cgtcgacccc
     1981 agtttgcatt gcggatgcaa agagcaacga taaggtactt cgttgccatc agcgtagacc
     2041 acttcaccgg tttcaatcgc ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa
     2101 tggtgatcgt caccaattcc ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact
     2161 gcaccaatat ccagctcctg atacagcacc tgtgccactt tcatgctgtt cacttcgtta
     2221 aacccctggc gcaaaatgcc ttccgtcgag gcagccactt ttagcgcagt ggcagaaaaa
     2281 gccgaagtgt atttttcaaa catcgcgcgt ttatcgagca atatacgcat aaacagcgcc
     2341 gcgccgacgg tattggtgac catcattggc gcagcaatat tactcaccag acgcaccgca
     2401 tcttcataag gtcgggcgat tgccaggatg atcaacattt gcaccatttc agcgacgaac
     2461 gtgacggcac cggcggtaat ggggttaaag actttatcag tgcgcccgcg acggatcagg
     2521 atgctgtgta ccaggccgcc gagcaaccct tcaacgatgg tcgagatcat gcaacttagc
     2581 gcggtcatgc cccccatcga atatcgatgt aacccgccgg tcagaccaac cagcccaccg
     2641 acgaccggac cgccgagtaa gccgcccatt accgcgccta tcgcacgggt attggcaata
     2701 gaatcgtcaa tgtgcaaccc aaaccaggtg cccatgatgc agaagatgga aaagacgatg
     2761 tagcagagaa atttatgcgg cagacgaacc gtgacctgca ttaacggtat gaataatggc
     2821 gttttactca ttaaccacgc aatgactaaa aaaacgcaca tctgctgaag cagcagcaac
     2881 accagattaa aatcgtacat acccgcaaac cacacttccc tttaaaacgc gtaacataca
     2941 ttgcctgcgt ttaactttct ttgaactctt gcagaaaaat gagaattcgt gagtacgatc
     3001 actcaaaatc gcctggcaaa aataaaatca ccctatagat gcacaaaaaa cgggcaaaac
     3061 tacctggttc gcaaaactgc gtctaaagtt aaaccgggac ctcgcgagca agggtgagac
     3121 gatggcgctt tacacaattg gtgaagtggc gttgctttgt gatattaacc ctgtcacgtt
     3181 acgcgcgtgg cagaggcgtt acggattgct gaaaccgcaa cggacagacg gcggccatcg
     3241 gctgttcaac gatgccgata ttgaccggat ccgcgagatc aaacgctgga tcgacaacgg
     3301 cgtgcaggtc agcaaagtta aaatgctgct cagtaatgaa aatgttgatg tgcagaacgg
     3361 ctggcgcgat cagcaagaaa cattactgac ttacctgcaa agcggcaatc tgcatagcct
     3421 gcgaacgtgg atcaaagagc gcggtcagga ttaccccgcc cagacgctca ccacacatct
     3481 gtttattcct ctgcgccgac gacttcagtg ccaacaaccg actctccagg cgctgctggc
     3541 gatcctcgac ggcgtactga tcaactacat cgccatttgc ctggcttcgg cacgtaaaaa
     3601 acagggtaaa gatgcgctgg tggttggctg gaatattcac gataccaccc gtctgtggct
     3661 ggagggctgg attgccagtc aacaaggatg gcgcattgat gtcctcgccc actcgctcaa
     3721 tcaactacgc cctgaactgt tcgaaggccg tacattgctg gtgtggtgcg gtgaaaatcg
     3781 aacctccgcc caacagcagc aactcaccag ttggcaggaa caaggccatg atattttccc
     3841 actcggcatt taatcattcg ttaacaaatg cgctttactg tacaatcctt tcgttaacat
     3901 aaggagtgca tcatgcgcat agctaaaatt ggggtcatcg ccctgttcct gtttatggcg
     3961 ttaggcggaa ttggtggcgt catgctcgca ggttatacct ttattttgcg tgctggctaa
     4021 gcgcctgcac cagcctttca aacaggcggt ctgcaatgat cgccgccagt gccaccagta
     4081 acgccccctg gatcacatac gcggtattaa atccgctaag cccgatgatg atgggcgtac
     4141 ccagcgtgct ggcccctacc gttgaggcga tcgtcgccgt accaatgttg ataatcaccg
     4201 aagttcgcac gcccgccaga atcaccggag ccgccagcgg tagctcgacc ttacgcagtc
     4261 gctgaccacg gctcattccc atacctttcg caacttctgt cacgctgaca tcaatcgctc
     4321 ccagcccggc aagtgtcgcc tgcaggacgg gcagcacgcc gtaaaggatc aaggcgataa
     4381 tcgcgggttg cagaccaaag ccgatcaccg gaacggcaat cgccagcacc gcaacgggcg
     4441 gaaaagtctg cccaacggcg gcaatagttt ccaccagtgg gcgaaattcc gcgccccacg
     4501 ggcgagtaac aataattccg gcaccagtac caatgatcac cgcaaacaaa ctcgaaattc
     4561 ccaccagcca gaaatgagcc agtgccagag ctgcaaagct ttcctgctga taaacgggtc
     4621 gtggcagttg tgggaacaag gcagcaaaca gcggctggct gtaaggcagc cagaaaagca
     4681 gtgccacaaa caaagcaatg agccagaaca gcggatcgcg caacatcttc atacgcttac
     4741 gcctccacca gcagatcctg aaaatgcagc gtgccgcaag gctggccctg cgtgttcacc
     4801 accggcagca cctcgcatcc ccgcgcgaca aacagggaga gcgcatcgcg tagcgtcatc
     4861 tcttctgcca gtgcctcacc ttctgcccgt tcttcgcgac gcacgtaatc cgccacacta
     4921 cgtaacgaaa gcaggcggac acccagttca ctatgcccaa aaaactggcg gacaaaatca
     4981 ttcgccggac gagtcagcat cgtcagcgga ttgccctgct gcaccacttc accgtgatcc
     5041 atcaacacca gatgttctgc cagtcgtagc gcctcatcaa tatcatgagt gaccagcaca
     5101 atggtacgcc ccagcaaacg gtgtatgcgc gtcatctctt gttgcaacgc gccgcgcgtt
     5161 accgggtcca gcgcgccaaa aggttcatcc atcagtaaga cttgcggatc ggcagccagt
     5221 gcgcgcgcca ctcccacacg ttgctgctga ccaccggaaa gctgatgcgg ataacgctca
     5281 cgcaaatttg gctccaaccc cagtagcgcc attaattcgt cgatacgatc gtcgatccgc
     5341 gcccgtgacc atttttgtaa ttgcggcacg gtggcaatat tttgcgccac gctccagtgg
     5401 ggaaacaggc caatagattg aatggcatag cccatccggc ggcgcaactc cagcactggc
     5461 agcgagcgaa tttcttctcc ggcaaagcgg atcacgccac tgtcatgctc caccaggcgg
     5521 ttaatcattt tcagagtggt ggatttaccg gagcctgatg taccaatcag caccgaaaaa
     5581 ctcccctcct gaaaattgag attgagatcg ttaacggctt tttgtatgcc gaacagtttg
     5641 ctgacatggc taaattcaat cattacgttt caccttcagc agtgcgataa gcaaatcgaa
     5701 cagcgcatcg atcagcaccg ccagaacaat taccgggatc acccctagca acactaaatc
     5761 aatggcactg cttagcagcc cctggaaaac cagcgcacca aaaccgcctg cgccgattaa
     5821 cgccgcaatc accgccatgc cgacggtttg caccatcacc acccgcaggc tacgcagaaa
     5881 taccggtaac gccagtggta actgaacatg caggaatcgc tgcgccccgc tcatgcccat
     5941 cgctctggcg ctctccagca catcgcgcgg gatctggttc aagccgacta ccacgccgcg
     6001 caccagcggc agcaaggcat agagcaccag cgcaatcagt gcgggtgtca ttccggttcc
     6061 tgctatgccg agcttaccca gccacggaaa ggccgtaacc agtgcggcaa gcggcgcaat
     6121 caacaggcca aagagcgcca ccgaaggcac ggtctgaatg acattcagca gagaaaaaat
     6181 cgccccctgc cgcgcagtgg aaaagtagca ccagacgccc agcggcacac caatcactaa
     6241 cgcaggcagc accgcaccaa acagcaacgt cagatgttgc gccagcgcgt catcaaacac
     6301 atcctgacgg ttggcgtatt ctttcattag cgagagatcg ttaagcgcgc cggaatacag
     6361 caaccacagc ggaatgatgg caatctgcat atgcaacaac cagcgccaca gcggatgcgt
     6421 ggagattcgg cggatggcat cgctacaggc cagcaatgcc agcgccgcag ccagccagaa
     6481 accactgccg aggctggtac gcgccagcgc actgccattt tgtgccagtt gtgtcgccgc
     6541 ctttcctgct ccccacacca gcaatacgaa gacgaattgc gccagaatga gtgcaaaaat
     6601 gctgcctttt ttagcgggaa taaaacaggc tgtcatccag gcgcaaccaa cgcccaccag
     6661 catccagatc gtttgcggcc acagctgcca gagatgacgc ccctcacccg aaactaaacg
     6721 attaggcgcg taactgataa acggcagcgc cgctgcgatt gccgtcagca acagcagcag
     6781 cgccagaaca ggattaatac ggaaataagt cacgggaaat tacttcgtcc accctttttg
     6841 tttcaggtag tcggcagcca cttttttggc atccagtcct tccacagcaa tgctggcatt
     6901 cagttgctgc aatgtttttt catcgaggct ggcgaagact ggctgtagcc actgtgccat
     6961 ttgtggatac tctttcaaca ccgactcacg caccactggt gcaggcgcgt agataggttg
     7021 cacgccttgc ggatcgctta aggtttgcag ccccagcgcc gcgaccgggc cgtcagtgcc
     7081 gtaagccatc gccgcattaa cgccagaggt ttgctgggca gcggctttga tcgtcaccgc
     7141 cgtgtcgcca ccagccagtg acagcaactg atcctgaccg agcttaaagc cataggcttt
     7201 ttcaaacgcg ggtaacgcat cggcgcgttc aataaattct gccgaggccg ccagtttgaa
     7261 ggtgccgccc tctttcagat aacgactcag gtcggcaagc gaagtgagtt tgtttttctc
     7321 tgccacatct tgacgcacgg cgatagtcca ggtgttattt gcaggcgcgg gcgtcagcca
     7381 gattaacttg ttttgctctg catcgagttt tttgactttc tcgtagcctt gcccggcatt
     7441 tttccatgcc gcatcgtttt catctttaaa gaaaaacgcg ccattgccgg tatattccgg
     7501 atagatatcc agttcaccgg aagtaatcgc cccgcgcacc acgggagtcg ttcccagttg
     7561 cactttattt accgtaggaa caccgtgact ttccagcacc tgcaaaatga tattgccgag
     7621 cagcgcgcct tcggtatcga tttttgaacc gaccttaacg ggggaagccg cctgtagcgg
     7681 caggctcacg gctgccaaca aaaccaatga acctgcccag agctttgaga gtggcatgat
     7741 gctttcctca ttcttttact gttgttttca gcgaattaag agaaaagcat agttgataat
     7801 gacagggtta gcctgtctgg gcggattcag ttgcagaatc agataaatac ttaaagggcg
     7861 cagacgtcgc gcccttacta aagcattaca gcaactcaaa ctcgcctttc ttaacgcgtg
     7921 cggaatcagt gccgataaag acattgaact tgccaggctc ggcgtcatat ttcatctgtt
     7981 gattccagaa cttcagcgcc tcgatatcaa tcgggaagct gacggtctgg gtttcgcccg
     8041 gtttcagggt gattttctca aagcctttca gctgcttcac cggacgactc atggaagccg
     8101 tcacatcctg caagtacatc tgcactaccg tcgccccttc gcgcttaccg gtgttcgtca
     8161 cctgcacact ggcggtcact ttgccgtcac gcttcatggt cggcgcagaa agtttcacat
     8221 cagagacggt gaaagtggta tagctcagac catagccgaa cggataaagc gccccgttag
     8281 cttcatcaaa ataacgcgaa gtgtatttgt tcggcttatc ggcgttatac gggcgaccgg
     8341 tattcagatg gctgtagtac acagggatct gcccgacaga acgcgggaag gacatcggca
     8401 gcttgccgga cgggttgtaa tcgccaaaca atacatcggc aatcgcattc cccccttcag
     8461 tccccgcaaa ccaggtttcc agaatcgcat cagcctgctg atcttctttc accagcgcca
     8521 gcggacgccc attcatcagc accagcacca gcggtttacc ggtggctttc agcgccgcaa
     8581 tcaagtcacg ttggctttgc ggaatagtga tatcggtacg gctggaagcc tcgtgcgcca
     8641 tcccctgtgc ttcaccgacc acagccacca ccacatcaga ttgtttcgcg gtttgcaccg
     8701 cttcatcaat catctcttgc ggcgaacgcg gatcgacttt gaccgcttct tcatactgat
     8761 tcaggaaatc gataatgcct ttgtcactgg taacgttagc ccctttggca tacagcactt
     8821 taccgttttc accgacggca tttttaatcc cggtcagcac ggtcacggat tgatcggcaa
     8881 cccctgccgc cgaccagctg cccatcacgt cacgcttgct gtctgccagc gggccaacca
     8941 ccgcaatggt tgcggatttt ttcagcggta acgtttcgag acggtttttc agcaacacca
     9001 ggctttcgcg cgccacttca cgcgcttctt tgcggtgcag gcggctttcg gcattggtat
     9061 ccaccgggtc agactctttc ggccccaaat ggctgtatgg gtcgttaaac aaccccatat
     9121 catatttaac gttcagtacg tggcgggctg catcgtccag ctctgccatg gtcactttgc
     9181 cggatttgat caacccaggc agatacttcg agtaatactc gtcgctcata ctcatgttga
     9241 tgccggattt cagcgccacg cgcaccgcgt cttccgggtc tgccgccgtg ccatgtttaa
     9301 tcagctcttt gattgcgccg tgatcggaaa cggtgatgcc tttaaagccc cactggtcgc
     9361 gcagaacatc tttcagcagc caggagtcgg aggtggctgg cgtaccgttc agcgagttca
     9421 gcgccaccat caccgcgccg ctgcctgcct ccagccccgc tttgtacggc ggcatataat
     9481 cattaaacag gcgctgcgga ctcatatcga cggtgttgta ctctttaccg ccttctaccg
     9541 cgccgtaagc ggcaaagtgt ttgacgctgg tcatcaccga gtagcgatct gccggacttt
     9601 taccctgcat cgcttccacc atggttttcc ccattattga ggtgagatac gtatcttcgc
     9661 caaaaccttc ggaagcacgt ccccagcgcg gatcgcgcga gacatcgacc attggtgccc
     9721 aggtcatatt caggccatca tctgccgctt cataagcaga gacacgcccg actgttttca
     9781 ccgcatcgag gttaaaagac gaggccagac cgaggctaat cgggaacacc gtgcgctgac
     9841 cgtgcagcac gtcgtaagca aagaaaagag gaattttcag gcggcttaat tccatcacct
     9901 gatcctgcat ggcgcggata tcctgacggg ttacggtgtt gaaaatcgcc ccaacctgcc
     9961 cgtctttgat catctcgcgg atcgcctctt tcgggttatc cgggccgacg ctgattaagc
    10021 gcagctgacc aattttctca tcaactgtca ttttcttaag cagttcggtg acgaacgcat
    10081 cccgcgcttc aggcgttaat ggatggttac cgaataaatc atccgccagt gctggctgca
    10141 gagccagact caccgcgatt cctactgaac atagccattt catatggatt tttcttcctt
    10201 tgttgcccga cgtggcagcg aaaatggtgc aaaaaccgta gtttgccata agcatgatgg
    10261 agagagaaaa agaatgctca gtttattgtc tgaattttca aaatattcac tcgctgaatt
    10321 gtcatacaag gcgctattct agtttgtgat attttttcgc caccacaagg agtggaaaat
    10381 gtcttccatg acaacaactg ataataaagc ctttttgaat gaacttgccc gactggtggg
    10441 ttcttcacac ctgctcaccg atcccgcaaa aacggcccgc tatcgcaagg gcttccgttc
    10501 tggtcagggc gatgcactgg ctgtcgtttt ccctggctca ctactggaat tgtggcgggt
    10561 gctgaaagcc tgcgtcaccg ccgacaaaat tattctgatg caggcagcca atacaggtct
    10621 gaccgaaggc tcgacgccaa acggtaacga ctatgatcgc gatatcgtga tcatcagcac
    10681 cctgcgtctc gacaagctgc acgttcttgg caagggcgaa caggtgctgg cctatccggg
    10741 caccacgctt tattcgctgg aaaaagccct caaaccgctg ggacgcgaac cgcactcggt
    10801 gattggctca tcgtgtatag gcgcatcggt catcggcggt atttgtaaca actccggcgg
    10861 ctcgctggtg cagcgtggcc cagcgtatac cgaaatgtcg ttattcgcgc gtataaatga
    10921 agacggcaaa ctgacgctgg tgaaccatct ggggattgat ctgggcgaaa cgccggagca
    10981 gatccttagc aagctggatg acgatcgcat caaagatgac gatgtgcgcc acgatggtcg
    11041 tcacgcccac gattatgact atgtccaccg cgttcgtgat attgaagccg acacgcccgc
    11101 acgttataac gccgatccgg atcggttatt tgaatcttct ggttgcgccg ggaagctggc
    11161 cgtctttgcg gtacgtcttg ataccttcga agcggaaaaa aatcagcagg tgttttatat
    11221 cggcaccaac cagccggaag tactgaccga aatccgccgt catattctgg ctaatttcga
    11281 aaatctgccg gttgccgggg aatatatgca ccgggatatc tacgatattg cggaaaaata
    11341 cggcaaagac accttcctga tgattgataa gttaggcacc gacaagatgc cgttcttctt
    11401 taatctcaag gggcgcaccg atgcgatgct ggagaaagtg aaattcttcc gtccgcattt
    11461 taccgaccgt gcgatgcaaa aattcggtca cctgttcccc agccatttac cgccgcgcat
    11521 gaaaaactgg cgcgataaat atgaacatca tctgctgtta aaaatggcgg gcgatggcgt
    11581 cggtgaagcc aaatcgtggc tggtggatta tttcaaacag gccgaaggcg atttctttgt
    11641 ctgtacgccg gaggaaggta gcaaagcgtt tttacaccgt ttcgccgctg cgggcgcagc
    11701 aattcgttat caggcggtgc attccgatga agtcgaagac attctggcgc tggatatcgc
    11761 tctgcggcgt aacgacaccg agtggtatga gcatttaccg ccggagatcg acagccagct
    11821 ggtgcacaag ctctattacg gccattttat gtgctatgtc ttccatcagg attacatcgt
    11881 taaaaaaggc gtggatgtgc atgcgttgaa agaacagatg ctggaactgc tacagcagcg
    11941 cggcgcgcaa taccctgccg agcataatgt cggtcatttg tataaagcac cggagacgtt
    12001 gcagaagttc tatcgcgaga acgatccgac caacagtatg aatccgggga tcggtaaaac
    12061 cagtaagcgg aaaaactggc aggaagtgga gtaataatta cggatggcag agtatcgcca
    12121 tccgtaattc atttaatcgt tctgtgccgt ctgccccgcc gccgccattt gggcggcttt
    12181 ttgtttttta tagctcaacg ctgctgccgg aacaggcatc actttaccgg tttcaatcca
    12241 ggtacgcagg cggctggcat cggcaaaatg ggtatatttg ccaaacgcgt ccatcactac
    12301 cagcgccacc ggtttattat tgataaccgt acgcatcacc agacaatggc ccgccgcatt
    12361 ggtaaagccg gttttggtta actgaatatt ccagttatcg cgatacacca gatggttagt
    12421 attgcggaac ggcagcgtat acgtcgggtt agagaaggtt gccatatctt cccgggtggt
    12481 acttaactgc ccgatcaacg gatattgttt gctggcaatg agcagtttgg ttaagtcacg
    12541 ggcggttgaa acgttatgca ccgacaatcc ggtcggttca acaaagcgcg tgttgttcat
    12601 tccgagcgat ttcgctttcg cattcatcgc cttaataaag gctttgtaac caccgggata
    12661 atggtgcgca aggcttgccg ccgcgcggtt ttctgaagac atcagcgcca gcaacagcat
    12721 atctttacgg ctgatttcgc tattcagtcg tacgcgcgaa tagacccctt tcatctccgg
    12781 cgtctggctg atatccactt ttagtttttc atccagcggc agtcgtgcat ccagcacaac
    12841 catcgcggtc attaatttgc tgatagacgc aatcgggcgc accagatccg ggtggttcga
    12901 atagatcact ttgttggtat tcagatcaac aatcatcgcg ctaccggagg caatttccgg
    12961 ctgtgaagcg gtggtagcgg ctgccgtttt cgcaacggcc tgcggtgcaa aaggcacagc
    13021 cagcatcagg gccaggctaa ataaagaaac tcgaaatttc ggcatgatga gcattcagat
    13081 agtggttcac gcgcacgggt tgcgcaccgc cggagtaagg atttactgag gctagcgacg
    13141 ccatcataac gagcaaaaag tgcgatcgtc aaaggagaat cgtgaggaaa tgctgcattg
    13201 ctgacattta cgccagcaat gcaacacaaa agaactttct agaacaaacg ataaccgtaa
    13261 ccccatagta taacggtaag ggcgagcagc acttccagca ccagcacgcc tatcgccagc
    13321 gtcgaactgg agaagctaag gccttcctct ttgttgatat tcaggaagct cggaataccg
    13381 agataaagca ggtagccggt gtaaaacagc gccaccgtgc cgaccagcgc acacaaccag
    13441 accagcggat aaagcgccac cagaccgctt aaaaacagcg gagttgcaac gtagcccgcg
    13501 aagaccatgc agtgcgcaag tgacggacgc tgtgggtaat tgcgcgccat ccaccagatg
    13561 acccgcccca tcaccgcgac cccagccagc ataacgccgt aaaacaagac agccagcgcc
    13621 agtccggtaa accaggataa cttcagaata gtgccatcgc caaaattcca gccaatctgt
    13681 gtagtgccaa tgaaggcgca aatcaccgga atcgccgcca tcagcaaaac gtggtgggtg
    13741 tagtgatgag aaatcgtttc gttttcacga ttaatcacct gcatttcacg atcgggatgg
    13801 gaaaacagtc cccagacatg gctcataccg cccccttgtt gtgagttcat gaacctgaca
    13861 gttcaagtat aagccagctt gtgattattt tttgttcgct catataattt gcaccgtctg
    13921 atgtacgtcc tttttcagcg tgaactattc acagggtgta tgcttacagg caactaaagg
    13981 gagacagact ggcctatgga tctcaataca ctcatctcac aatatggtta tgccgcgctg
    14041 gtgatcggta gcctggcgga aggtgaaacc gtgactttgc tgggaggcgt tgcggcacat
    14101 caggggctat taaagttccc gctggtggta ctttctgtgg cgcttggcgg catgattggc
    14161 gaccaggtgc tttatctgtg cgggcggcgg tttggcggca agctgttacg ccgtttctcg
    14221 aaacatcagg ataaaattga gcgggcgcag aaacttatcc aacgccatcc gtatctgttt
    14281 gtcattggta cgcgttttat gtatggcttt agggtgattg gcccgacgct gattggtgcc
    14341 agccagctgc cgccgaaaat ctttctgcca ctgaatatcc ttggcgcatt tgcctgggcg
    14401 ttgattttta ccactatcgg ttacgctggt ggtcaggtga ttgcgccgtg gttgcacaat
    14461 ctcgaccagc atttgaagca ctgggtctgg ttgattctgg tcgtggttct ggtggtaggc
    14521 gtgcgctggt ggctgaaacg acgcgggaag aaaaagccgg ataatcaggc gtaaaaccat
    14581 tgcctaggat aaggcgttcg cgccgcatcc gacatccggt gcaccatgcc tgatgcgacg
    14641 cttgtgcgtc ttatcatgcc tacccaaata ttacccattg aaataaccac gcacctcact
    14701 gcgctattct gggttgaact gcggattcgc cagcataaag ccgccatcca ctatcaacga
    14761 ctgcccagtg gtgtaatttg cgccctccga acaaagccac accaccaggc tggcaatctc
    14821 atgcgttgcg ccaaaacgcc gcaagggaat cgaaggctcc gcgccgggct tcacgtcgcc
    14881 atcatccatg ccgttcattg gcgtggcgat cgccccaggt gcaaccgcat tcaccaaaat
    14941 cttatgcctg accagctcca gcgccatcgc tttggttaac ccgccgagcg catgtttagc
    15001 ggctgtgtag gcgctggcat ccggcaacgg cgtatgttca tgcaccgacg taatgttgat
    15061 gatgcgacca ccctgccctt gtttcaccat ctggcgggca gcaatttgcg agcataaaaa
    15121 tgcaccatcg acatcaacgg taaaaatctt gcgccactca tcgaaagcca tatcaagaaa
    15181 cggcgctttg gtcattgcac ccgcattatt caccagcaca tcaatacgcc ccagccgttg
    15241 aatgagtttc tccagcgcca gtgccccttc tggtagattg ccgagatcca gctgcacgat
    15301 ctccgcacgt acgccgtggc taactacctc acgcgcggta tcttttgccc cttcttcatc
    15361 tgagtgccag gtaataccaa tatcaaaccc ctgctgcgcc agtaataacg cgcactcttt
    15421 gccgatcccc gaatcggaag cggtaataat cgcaacctgt gccatcgagt tctccactta
    15481 acgctgaata aacgttaagt atagaaggcg catatcatca gcgtttgtaa cccccgccca
    15541 acgcaccagt gagttgaatg gaggcatcca gccactgccc ttgcaataac aggccattgg
    15601 cccgctcacg cagcgcgggg attctggctt cgctgacccg ggaacccgca atgaggcccg
    15661 cgttaaagcg cgcctgcgca agacctacta cacgcaaggc atcgcgctca atttgcgcct
    15721 gatgctggtt tttctccgcc agtgtctgaa cctgactggc tgcctgcgcc acgtcattca
    15781 ccgcttcaac caccgctttg ttgtagctgg cgatagacaa gttgctttcg gcttttgcga
    15841 tatcgaggtt ggcgttaaga cgaccactat cgaaaatggg tagcgtcagg cctgccgtaa
    15901 cgcccatttg ctgcgcggaa tgacggaaca gatcgcttaa gtgcaacgca tcctgttgca
    15961 ggaaggccat caggttgatg tcaggataaa atgccgcttt tgccgcatca atagtgctta
    16021 acgatgactc aacgtaccag tgcgccgcct gcaaatctgc ccgccgggcc agtaaggagt
    16081 accccagttc atcaggaagc tggcttgcca ctttcggcaa cgcgaccgga ttaagcttca
    16141 atgcctttgt ctggtgattt gtaagtgcgc ttaaccgtgc ctcaataatt ttcattttcc
    16201 ccgcgacatc gttgagctgc tgccgggttt tgctggcatt aatatcggtt tccacacctt
    16261 caactgaaga agtaatcccg ttctgatata gctggcgatc ggtggcgata atagtgttct
    16321 gctctttttc gatttgctgt aagaccgtgt ttaacgccgc ctgggtttgc cactcccagt
    16381 acagacgggc tacgctgcca gccagcaatt ggcgggtttg ctcgcgttcc gccgcccgtg
    16441 ctttaaccgt acccaggcga gcagtaacct ccgcccgatt ctttccccag atatcgagat
    16501 gccagcccgc cgttaagcca aaagtaccgt tggtgtacca cggaccggtc gtacctgcgg
    16561 caggatcgtt cagagcaaac ggccccatta acccttctgc agacattttt tgccgctcca
    16621 tatccgcaga aaagtcgatc tgcggaccat cctgagtggc aattgccttc gcctgggctt
    16681 cagccagctg aatgcgctgt tcagccacct gcatatccgg tgcgctctgt agtgcgttat
    16741 taattaagga agtgagttga ttatcgtgat actccagcca ccattggctg tctggccaac
    16801 catttttcag cgccgtgggt aatgcggtgt caacttgtgc agcgggcgtt tgctggctta
    16861 acgcctggcg ggtttcatgc ataggcgcac acccggtcag catcagtaac agcggaaaac
    16921 aggcgatggc tggataaaaa aaatcacgat tcatggggga ataatcaggt aagaaaaggt
    16981 gcgcggagat taccgtgtgt tgcgatatat tttttagttt cgcgtggcaa tacatcagtg
    17041 gcaataaaac gacatatcca gaaaaatata cactaagtga atgatatctt ccgatttatc
    17101 ttaatcgttt atggatagcg gcaaagggct tcgttttttc ctatacttat tcagcactca
    17161 caaataaagg aacgccaatg aaaattatac tctgggctgt attgattatt ttcctgattg
    17221 ggctactggt ggtgactggc gtatttaaga tgatatttta aaattaatta atgtcatcag
    17281 atccgaaaat aacgagaata tttcagtctc tcatcctgtt gcgctcctgt catgtgcatt
    17341 gcttcatata atcactggcg caaggagcgc gcagggggcg accaatcgcc gccgccccct
    17401 gca
//
LOCUS       DAPOZO010000095        17305 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPOZO010000095 DAPOZO010000000
VERSION     DAPOZO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.095"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(229..552)
                     /locus_tag="ROI21_003658"
     CDS             complement(229..552)
                     /locus_tag="ROI21_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDX5112534.1"
                     /translation="MKRNLMLCFVINALFSTSAMALTLNDARTQGRVGETYNGYLVAL
                     KADTETQMLVKRINESRNKSYQQLAMKNNLPVAEVEKLAGQKLVAKAKPGEYIRGING
                     MWLQK"
     gene            complement(560..745)
                     /locus_tag="ROI21_003659"
     CDS             complement(560..745)
                     /locus_tag="ROI21_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDX5112535.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(742..3381)
                     /locus_tag="ROI21_003660"
     CDS             complement(742..3381)
                     /locus_tag="ROI21_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDX5112536.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALNSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWLENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNNQLPMQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGIDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTSFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3589..4578
                     /gene="ldhA"
                     /locus_tag="ROI21_003661"
     CDS             3589..4578
                     /gene="ldhA"
                     /locus_tag="ROI21_003661"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDX5112537.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4689..5111
                     /gene="hslJ"
                     /locus_tag="ROI21_003662"
     CDS             4689..5111
                     /gene="hslJ"
                     /locus_tag="ROI21_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDX5112538.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGKLTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5108..5374)
                     /locus_tag="ROI21_003663"
     CDS             complement(5108..5374)
                     /locus_tag="ROI21_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDX5112539.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5648..9172
                     /gene="nifJ"
                     /locus_tag="ROI21_003664"
     CDS             5648..9172
                     /gene="nifJ"
                     /locus_tag="ROI21_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDX5112540.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELIPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPCG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDTNGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9539..10672
                     /gene="ompN"
                     /locus_tag="ROI21_003665"
     CDS             9539..10672
                     /gene="ompN"
                     /locus_tag="ROI21_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDX5112541.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10813..11247
                     /gene="uspF"
                     /locus_tag="ROI21_003666"
     CDS             10813..11247
                     /gene="uspF"
                     /locus_tag="ROI21_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDX5112542.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11832..12746
                     /gene="sitA"
                     /locus_tag="ROI21_003667"
     CDS             11832..12746
                     /gene="sitA"
                     /locus_tag="ROI21_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HDX5112543.1"
                     /translation="MYSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQEAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDMVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12746..13573
                     /gene="sitB"
                     /locus_tag="ROI21_003668"
     CDS             12746..13573
                     /gene="sitB"
                     /locus_tag="ROI21_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HDX5112544.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNASEESIITDDEHPFVAHRPSAVQREAR"
     gene            13570..14427
                     /gene="sitC"
                     /locus_tag="ROI21_003669"
     CDS             13570..14427
                     /gene="sitC"
                     /locus_tag="ROI21_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HDX5112545.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14424..15281
                     /gene="sitD"
                     /locus_tag="ROI21_003670"
     CDS             14424..15281
                     /gene="sitD"
                     /locus_tag="ROI21_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDX5112546.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFIAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDIHLDHILFGDMLGVTIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVISFNARTKGNA
                     HRQDLLSPN"
     gene            15754..16548
                     /locus_tag="ROI21_003671"
     CDS             15754..16548
                     /locus_tag="ROI21_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112547.1"
                     /translation="MDIRKDLESVAQYISRLLSIGYEFSRFDKDWVHLKNEEDFRFIS
                     RIPFATRNKVEAVYAEGRDMALYMSDELLSINSDFSKFPTLTAIIERFKDTWVYGNYD
                     SEVPNIAKKTCEENAVQLWSVEQMCSLFKKQEQLLAAVRITLQMLQDSDLYKMENGLP
                     LMKQEANIHVSGISGSSINIHSSGATATTTTNYNEPTIFNEMIEAIKSKNFDGATESH
                     LIDNVQALAASHQSGCFKEAYKDFINNVSAHITVFTPFLAGISSLL"
     gene            16673..16824
                     /locus_tag="ROI21_003672"
                     /pseudo
     CDS             16673..16824
                     /locus_tag="ROI21_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            complement(17094..>17305)
                     /locus_tag="ROI21_003673"
     CDS             complement(17094..>17305)
                     /locus_tag="ROI21_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112548.1"
                     /translation="GAIAGCTFFIAREHTQAEYRWIEMFGHGKRMNMPWWGGFDPRVW
                     DVASLMDFSVPVVACLLVWLLVNRG"
BASE COUNT         4507 a         4267 c         4098 g         4433 t
ORIGIN      
        1 atttatttca acttaaaata tggcgcaatt gagattgaat ccgataacac cgatgaatta
       61 aataatgcaa ttaattcatg aaataaataa attatcatgt gaatgaagca ttcatcatcg
      121 gcatctatat gaaattaaat gaaaaataat tctcaatagc attgcagtta atattgaata
      181 tttcaggagg tacaaccagg gagatttttt tcgacgaata ccgacttatt atttttgcaa
      241 ccacatcccg tttatcccgc gaatgtattc tccaggctta gcttttgcta ccagtttctg
      301 ccccgccaat ttttccactt ccgcaaccgg cagattattt ttcatggcga gttgttgata
      361 gctcttatta cgcgattcat taatcctttt gactaacatc tgtgtttcgg tatccgcctt
      421 taatgcaacc agataaccat tatacgtttc gccaacacgc ccctgagttc gagcgtcatt
      481 cagcgtcaga gccatcgcac tggtgctaaa taaggcatta atgacaaaac acagcattaa
      541 atttctcttc atcatcgcct cagaaaagat cgctacgagt ctcaagcagc tcttcgacat
      601 ctttgtctgc cttgatgatg atctcatgct caattttaac gttcatattg atagtgatcg
      661 gttccttagg cgcagcgact tcaatgcgag gcgtacagcc aaccagcata aaagatgacg
      721 tcaacgcagc cagtaaaatt ttcattgttt ttcctcacac tcttttcctt gcggacagtc
      781 atttcccggc agacgtgcgt tctgctccaa ccatgcctgg agattatcgc caaagcgtaa
      841 actgcgccac agcgtaaaaa tattctcttc atgatggtaa ttaaggttta ccgtaccgct
      901 tttaccatca acgcgactgg tacctgtaat gttggcctgc atggttaata aaccgagatt
      961 atctaaatta atatttgtcg atgaacggct aatttccata tagcgcaacc agttaattgc
     1021 tgaacccgca gtcatattgt ctttcaccac cgcatctgcg gtgtctttat ccagccgcaa
     1081 ggtcattggc ccgctatttg ccagccaacc atctttcact atccattttt cgttgtttag
     1141 ccataacggc aatgcaccac taaatgcccc ggacatggcg aattgtttcg gattgacggc
     1201 gctaaccagt tcgctggatg agagattatt cagccgcaac agcgccgggt catgttgcgg
     1261 catacgtaat tgtttcatca gcacgttacc gcctaacaca tcgacgctaa catcagtcaa
     1321 cagcaatggt tcttcttcag tccacggata gcgcccttgc aaatcagccg taatattttt
     1381 cgctgtcacc agattaatca cttcggcaat tcgcaacgta acggggccgc gagtccccag
     1441 atgccaggct ccatcggcaa aacggaaagg caggacaaaa tcgacaccgt taacctgatt
     1501 atctggcatc caggcactac cgcctttcaa cacgccgtgc cctcccgcgc ggaatccttg
     1561 ttcaggcgca gcagaaaatg caacctgagc atacagttca ccatcgcgta agttcatctt
     1621 ccagtcgggt ggcaccagcg gctggaatac agtcagtgat tgtttaggcc accaggcgtt
     1681 accgcgcaga cgaataccgt cccagcgacc atttacccga accgggccaa tctcaccagc
     1741 atgtaaatcg cctttaaaga ggaaatacgt cggatcgcgc ccatcgacgc taaattttaa
     1801 ggttgatggc ggtaacacac tgccgccagt gaacagcgtt tgcccggcgt ccagtgacag
     1861 cgcgccactg aaggagggat gctgtgcgtc acgtacccag acgacgggct tgtcgagaat
     1921 taatcgcggc ttttctaccg tcatcgtacc gtattgtaac tggtcgaaac tggtggagag
     1981 atcggtcagc gtaatcgtgc tgtcatgcca ctcacccttt cctgcgacat cccaggtggc
     2041 attcatcggt gtaaaactgc cttttcccca gtagcgccac tgccagcggc cagcgtcagg
     2101 gagaaaatca ttcgccagtc catccatatg cagcacgaaa tcgcccagtt cattttcatg
     2161 cgcctgcaaa atggcctgca aacgtccgtc aataccacgt tgggtgactt ttacacccgc
     2221 taaaggccag cggatttcat cgatgtccag cgaatcaatg actcttccct ttgaacgaag
     2281 taacgcgcca ggctcaaagg ccagcgttgg gtcagtcaga cttccgctta gctgcgcagg
     2341 taaacgggca tataaaatga ggtccgcctg tttcgcttca ccggtcagtt gcataggcag
     2401 ctggttgtta tccatgctta attttcctgg gccaaaatta agcaccgcgt tgcctttacc
     2461 cgcttgcccc tgggtcagca cactcagtcg tccgctgacc agagcgttct cgagccctgc
     2521 ctgccagttg tcgactttga cacccagtcg gccactcaaa ggaaaacctg catacggcca
     2581 actccagcga ccatcgctta cggtcaattg ttgacgagta atttgccacg gcaaatcgag
     2641 caacggatcg ccgttatccc gtgccagcac aatcaattgc ccgctatttt ccaaccagtc
     2701 cagctcggca tccaccagtg acggttcctg cggcaagttt aacgtcgcag tagcatgacc
     2761 acttacagga agtccatccg gcactagcgg catagtaaat tcccccacca gttttaccgg
     2821 cggctgattt tcaaacgcga cgacatccag ttcgctgact gtaagttgtt gccctttcag
     2881 ctggccttga aatttaactt tttcgccctg ataacgcagt tgctggatat cggagtttaa
     2941 tgcgagagag agttttccct gccattcctg ccagggagaa aaaattagtt tatcgatatt
     3001 gatccaggtg ttaggcaaca tggcctgcca ctgcgcgagg gtttttggag cggcaggcga
     3061 ttgctccgtc tgcggcaatt tcgccagaca agcagaatca agttctaccg tgccgacgtt
     3121 caataaccag cggctcggat gtgaaaggct ggcgttggtg atatgcgcaa gctgacaatc
     3181 tcccaccaga taacggagat cgggaatgat taaaccttta cgcgtaatgc gtgggctttc
     3241 atctaatgca atacgtgtcc cgagcggtag ccagatgccc gccagtgtgg gaacccacaa
     3301 gccgagcgtc atcagcagcg tcaacggcac aagaataatc agtaataaca gcgcgagaac
     3361 ggctttatat ttacccagca tgggtagtta atatcctgat ttagcgaaaa attaagcatt
     3421 caatacgggt attgtggcat gtttaaccgt tcagttgaag gttgcgccta cactaagcat
     3481 agttgttgat gaatttttca atatcgccat agctttcaat tatatttgaa attttgtaaa
     3541 atatttttag tagcttaaat gtgattcaac atcactggag aaagtcttat gaaactcgcc
     3601 gtttatagca caaaacagta cgacaagaag tacctgcaac aggtgaacga gtcctttggc
     3661 tttgagctgg aattttttga ctttctgctg acggaaaaaa ccgctaaaac cgccaatggc
     3721 tgcgaagcgg tatgtatttt cgtaaacgat gacggcagcc gcccggtgct ggaagagctg
     3781 aaaaagcacg gcgttaaata tatcgccctg cgctgtgccg gtttcaataa cgtcgacctt
     3841 gacgcggcaa aagaactggg gctgaaagta gtccgtgttc cagcctatga tccagaagcc
     3901 gttgctgaac acgccatcgg tatgatgatg acgctgaacc gccgtattca tcgagcgtat
     3961 cagcgtactc gtgacgccaa cttctctctg gaaggtctga ccggctttac tatgtatggc
     4021 aaaacggcag gcgttatcgg taccggtaaa atcggtgtgg cgatgctgcg cattctgaaa
     4081 ggttttggta tgcgtctgct ggcgttcgat ccgtatccaa gtgcggcggc actggaactc
     4141 ggtgtggagt atgtcgatct gccaaccctg ttctctgaat cagacgttat ctctctgcac
     4201 tgcccgctga caccggaaaa ctaccatctg ttgaacgaag ccgccttcga tcagatgaaa
     4261 aatggcgtga tgatcgtcaa taccagtcgc ggtgcattga ttgattctca ggcggcaatt
     4321 gaagcgctga aaaatcagaa aattggttcg ttgggtatgg acgtgtatga gaacgaacgc
     4381 gatctgttct ttgaagacaa atccaacgac gtgatccagg atgacgtgtt ccgtcgtctg
     4441 tctgcctgcc acaacgtgct gtttaccggg caccaggcat tcctgacagc agaagcactg
     4501 accagtattt ctcagactac gctgcaaaac ttaagcaatc tggaaaaagg cgaaacctgc
     4561 ccgaacgaac tggtttaatc ttgccgctcc cctgcattcc aggggagctg attcagataa
     4621 tccccaatga cctttcatcc tctattctta aaatagtcct gagtcagaaa ctgtaattga
     4681 gaaccacaat gaagaaagta gccgcgtttg ttgcgctaag cctgctgatg gcgggatgtg
     4741 taagtaatga caaaattgct gtaaccccag aacagctaca gcatcatcgc tttgtgctgg
     4801 aaagcgtaaa cggtaagccc gtgaccagcg ataaaaatcc gccagaaatc agctttggtg
     4861 aaaaaatgat gatttccggc agcatgtgta accgctttag cggtgaaggc aaactgtcta
     4921 atggtaaact gacagccaaa gggctggcaa tgacccgtat gatgtgcgct aacccgcagc
     4981 ttaatgaact cgataacacc attagcgaaa tgctgaaaga aggtgcacaa gtggatctga
     5041 ccgcgaacca gttaacgctg gcgaccgcga aacagacatt aacttataag ctagcggatt
     5101 taatgaatta atagctgcca cagctcccgg cggcaagtga ctgttcgcta cagcgtttgc
     5161 cgttgggtaa tgcacacatc ccaatcgccg taccatccag ttgacgggca acagaaagcg
     5221 aaccgccgat cattgcacaa tttgcttctc cactactgga catcgacgct tttaaacctg
     5281 gcgctacgtg cgccgcagtc gcctgctgaa caggttcact actacacgcc gacaacaata
     5341 aagcggcaca ccctacccaa aacgctgctc gcatcttttc ttcctctgat cttcaagcca
     5401 aacgacaccg ccataaataa taggcagcac agagggcggc gtcgagagct gtcctgcgcg
     5461 ttgccccgca tttttacttt tttatggcta tttttttgcc ctctgtttga tcaaaacatt
     5521 cattacgctg atgaggggga cacaaaagcg aaaatgcaga agaaagccat ttgctaaaat
     5581 tgaaggatta ctactgggcg cgcagcaatt tcgtgcgccc ctcatttgcg caatgtaagg
     5641 gtgtcatatg attactattg acggtaatgg cgcggttgct tcggtggcgt ttcgcaccag
     5701 tgaagttatc gccatctacc ctattacccc cagttccacg atggcagaac aggctgatgc
     5761 atgggccgga aacggcttga agaacgtttg gggagacaca ccacgcgtgg ttgaaatgca
     5821 gtcggaagcg ggggctatcg ctaccgtgca tggcgctttg cagacgggtg ccctttcaac
     5881 atcgtttacg tcatcgcagg gtttgctgct gatgatcccg acgctgtaca aactggcagg
     5941 cgaactgacg ccatttgtcc tgcatgtagc ggcacgtacc gttgccacac atgcactctc
     6001 tatttttggc gatcattccg acgttatggc ggtgcgccag acgggttgcg cgatgttgtg
     6061 tgcggcaaac gtccaggaag cgcaagactt tgctcttatt tcgcacatcg cgacgctgaa
     6121 gagtcgcgtg ccatttattc atttctttga tggtttccgc acgtcccacg aaatcaataa
     6181 aattgtcccg ctggccgatg acacgattct tgaactgata ccgcaggccg aaattgatgc
     6241 ccaccgcgcc cgcgcgctga atccggagca tccggtgatt cgtggtacct cggctaaccc
     6301 ggacacctat ttccagtcac gtgaagccac caacccgtgg tacaacgcag tttacgacca
     6361 tgttgaacag gcgatgaatg atttctctgc cgcgacaggt cgtcagtatc agccgtttga
     6421 gtattacggg catccgcaag cggaacgggt gattatcctg atgggctctg ccattggcac
     6481 ctgtgaagaa gtggttgatg aattgctaac ccgtggcgaa aaagtcggcg tgctgaaagt
     6541 tcgcctgtac cgtcccttct ccgccaaaca cttgctacaa gctctgccgg gatccgtacg
     6601 cagcgtggcg gtactggaca gaaccaaaga acccggtgcc caggctgaac cgctctatct
     6661 ggatgtgatg accgcactgg cagaagcctt taataatggc gagcgcgaaa ccctgccccg
     6721 tgtcattggt gggcgctatg gtctttcatc caaagaattt ggcccggact gtgtactggc
     6781 ggtatttgcc gagctcaacg cggctaaacc gaaagcgcgc tttacggttg gtatttacga
     6841 tgatgtgacc aatctgtcac tgccgttgcc ggaaaacacc ctgccaaact cggcgaaact
     6901 tgaagccttg ttttatggcc ttggtagcga tggcagcgtt tccgcgacca aaaacaatat
     6961 caagattatc ggtaattcca cgccgtggta cgcacagggc tattttgttt acgactccaa
     7021 aaaggcgggc ggcctgacgg tttctcacct tcgcgtgagc gaacagccga ttcgttccgc
     7081 ttatctcatt tcccaggctg attttgttgg ctgccaccag ttgcagttta tcgataaata
     7141 tcagatggct gagcgtttaa aaccttgcgg cattttcctg ctcaacacgc cgtacagcgc
     7201 agatgaagtg tggtcgcgct tgccgcaaga agttcaggcc gtgttaaacc agaaaaaagc
     7261 gcgcttctat gtgattaacg cggcgaaaat cgcccgcgaa tgtggcctgg cggcccgtat
     7321 taataccgtc atgcagatgg cttttttcca tcttacgcaa attctgcctg gcgatagcgc
     7381 cctcgcagaa ttgcagggtg cgattgccaa aagttacagt agcaaaggtc aggatctggt
     7441 ggaacgcaac tggcaggctc tggcgctggc acgtgaatcc gtagaagaag ttccgctgca
     7501 gccggttaat ccgcacagcg ccaatcgacc gccagtggtt tccgatgccg cccctgattt
     7561 cgtgaaaacc gtaaccgcgg cgatgctcgc cgggcttggt gatgccctcc ccgtttcggc
     7621 gctgccgcca gacggcacct ggccgatggg caccacgcgc tgggaaaaac gcaatatcgc
     7681 cgaagagatc cccatctgga aagaggaact ctgtacccaa tgtaaccact gcgtcgccgc
     7741 ttgcccacac tcagctattc gcgcaaaagt ggtgccgcct gaagcgatgg aaaacgcccc
     7801 tgccagcctg cattcgctgg atgtgaaatc gcgtgatatg cgcgggcaga aatatgtctt
     7861 gcaggtggct ccggaagatt gcaccggttg taacctgtgc gtcgaagttt gcccggcgaa
     7921 agaccgtcag aatccagaga ttaaagccat caatatgatg tcgcggctgg aacatgtcga
     7981 agaagagaaa atcaattacg atttcttcct caacctgcca gaaatcgacc gtagcaaact
     8041 ggaacgtatt gatattcgta catcgcagct gattacaccg ctgtttgaat attccggtgc
     8101 ttgctccggt tgtggcgaga cgccgtatat taaattgctg actcagctct atggcgaccg
     8161 gatgttgatc gctaacgcca ccggctgttc ttcaatttat ggcggtaacc tgccctctac
     8221 accgtatacc accgatacca acggtcgcgg gccggcatgg gcgaactctc tatttgaaga
     8281 taatgccgaa tttggccttg gtttccgcct gacggtcgat caacaccgtg tccgcgtgct
     8341 gcgtctgctg gatcaatttg ccgataaaat cccggcggaa ttactgactg cgttgaaatc
     8401 agacgccacg ccagaggttc gtcgagaaca ggtcgcagct ttacgccagc aactcaacga
     8461 tgttgccgaa gcacatgaac tgctacgtga cgcagatgca ctggtggaaa aatcaatctg
     8521 gctgattggt ggcgacggct gggcttacga tatcggcttt ggcggtctgg atcatgtatt
     8581 gagtttgacg gaaaacgtca acattctggt gctggatacg caatgctatt ccaataccgg
     8641 tggtcaggcg tcaaaagcca cgccgcttgg cgcggtgact aagtttggcg agcacggcaa
     8701 acgtaaagcg cgtaaagatc ttggcgtcag tatgatgatg tacggtcatg tttatgtggc
     8761 gcagatttct ctcggcgcgc agctgaacca gacggtgaaa gcgattcagg aagcggaagc
     8821 gtatccgggg ccatcgctga tcattgctta tagcccgtgt gaagagcatg gttacgatct
     8881 ggcactcagc cacgaccaga tgcgccaact cactgccacc ggcttctggc cgctatatcg
     8941 cttcgatcca cgtcgagccg atgaaggcaa actgccgctg gcactggatt cacgcccgcc
     9001 gtcagaagca ctggaagaaa cgttacttca cgagcagcgt ttccgtcgtc tgaattcgca
     9061 gcagccagaa gtggcagaac agttatggaa agatgctgca gctgatttgc aaaaacgcta
     9121 tgacttcctg gcacaaatgg ccggaaaagc ggaaaaaagc aacaccgatt aaatgcactg
     9181 gataaggatt atccaattct aaaaaaaagc ccggacgact gttcgggctt gtctcattat
     9241 ttgttggaaa atcagtagca acgcaatggc ccaacagaaa ataaaatctg tgacaataaa
     9301 ggcatataac ccgcgcagaa taacgtatag caaattaaaa gtataatttt tattttgtat
     9361 gtattatctt caatacattc accagactta ttatcatttc agaagaatta tttgattaag
     9421 gttttactta aggcgtaaca aatgatattt atcggctaac tgaacttctc ctttaggatg
     9481 ctttcactcc ccaaaatggg gatgaaaagg caaataaaat aactaaagga tttattcaat
     9541 gaaaagcaaa gtactggcac ttttaattcc tgccctgctc gccgcaggtg ctgcacatgc
     9601 agccgaagtt tataataaag acggcaacaa attagatctg tatggcaaag ttgatggcct
     9661 gcattatttt tctgataatt cagcgaaaga tggcgaccag agctatgcgc gtctgggttt
     9721 taaaggcgaa acccaaatta acgatcaact tactggctac ggtcaatggg aatacaatat
     9781 tcaggcaaac aacactgaat cttcaaaaaa ccagtcatgg acccgtctag catttgccgg
     9841 actgaaattt gcagattacg gttctttcga ttacggacgt aattatggcg ttatgtacga
     9901 catcgaaggc tggaccgata tgctgcctga atttggcggt gactcttata ccaatgcaga
     9961 caactttatg actggtcgag ccaatggcgt cgcgacttat cgtaatactg atttcttcgg
    10021 tctggtaaat ggtctgaact tcgcggtgca gtatcaaggt aacaacgaag gtgccagtaa
    10081 tggtcaggaa ggcaccaaca acggacgtga tgttcgccat gaaaacggtg acggctgggg
    10141 tctttccaca acatatgatt taggcatggg ctttagcgct ggtgcggcat acacctcttc
    10201 tgaccgcacc aatgaccagg ttaaccatac tgcggcgggt ggtgataaag cagacgcgtg
    10261 gactgctggg ctaaaatacg atgctaacaa tatttacctg gcaaccatgt attcagaaac
    10321 gcgtaatatg accccgtttg gcgacagcga ttatgctgtt gcaaacaaaa cccagaactt
    10381 tgaagttact gcacagtacc agtttgattt tggtctgcgt ccggcagtct ctttcctgat
    10441 gtctaaaggc cgtgacctgc acgctgccgg tggtgcagac aacccagcag gtgttgatga
    10501 taaagatctg gttaaatacg ccgatgttgg cgcgacttac tatttcaaca aaaatatgtc
    10561 cacctacgtt gactataaaa tcaacctgtt ggatgaagat gacagcttct acgctgccaa
    10621 cggcatctct accgatgata ttgtcgcttt aggtctggtt tatcagttct aaatcctcct
    10681 gcccgctgtt atggcgggct ttttctgctt attcttcctc ctttgatcta aattaaaaat
    10741 gtgaactccg tcattacaca aaaagtgtca tctggcgtta cactttatgc ggatactaaa
    10801 acaggaggtt ttatgaacag aacgattctt gtccctatcg atatttccga ttcagaatta
    10861 actcaacgcg tgattagcca cgttgaggca gaggcaaaga ttgatgatgc agaggttcat
    10921 ttcctgacgg taataccttc actgccctac tatgcctctc tgggtttagc gtattccgca
    10981 gaattaccgg caatggatga cctgaaagcg gaagccaaat cgcaactgga agagatcatt
    11041 aaaaaattta aactgccaac cgacagagtg catgtccatg ttgaggaagg ctcgcccaaa
    11101 gaccgcattc tggaattggc gaagaagatc cccgctcata tgatcatcat tgcttcccat
    11161 cgaccggata tcaccactta tctgctcggt tccaacgccg cagctgtcgt gcgtcacgca
    11221 gagtgctccg tgctggttgt gcgctgacac taacgcccgc acattgctgc gggctttttg
    11281 attcatttcg caaatgtgct gacattttcc cctctaatcc gtaccataca cgccacagtt
    11341 tttatatcag atttcttatg ctgggcgttc cggcatgacg catactcttc tgatgccata
    11401 taacgaattg agtcgctttt aaatgtcgca aaatcaagaa attagtaaga aagaacaata
    11461 caacctgaac aagtaagggc aaaaatcaca actctctgac tcataagtat tttacttatt
    11521 tttcaatgtg ttaaatatca tcagcgacaa caataagcta cgataaccca ctcttttttt
    11581 gccccttttt tgcccctttt gcagcgcttt gccccatttt tgccatcaac aattttcctc
    11641 atcaagtcat ttcaaagcct aataggtgcc ctactttttt cctcagtacc acgtcgccag
    11701 caggaaacaa cctaatcccg gttcggatct aaaattgcta atgataatca ttttcatttt
    11761 gtattgctta tgctataaaa catagccgtt gctatgtttg ttctaatttt gctcactata
    11821 ggtactaaat tatgtactca ataaaaaaag taaccatgct cttggggtgc ctcgccctca
    11881 cctgctcgat cgcatttcag gcgagtgcaa ctgaaaaatt caaggtcatt acaacattta
    11941 ccatcatcgc agatatggcc aaaaacgtgg ctggagatgc tgcagaagtc tcatccataa
    12001 ccaagcccgg tgcagaaatt catgagtatc agcctacccc tggcgatatt aaacgtgcgc
    12061 aggaggcaca actgattctc gccaatggta tgaatctgga attgtggttc caacgctttt
    12121 accagcatct taatggggtt ccagaagtaa ttgtctcttc gggtgtgacg ccagtaggga
    12181 tcactgaagg cccctatgag ggcaaaccta acccccatgc ctggatgtcg ccagataatg
    12241 ctctgattta cgtcgataat attcgtgatg cgttgataaa atacgatccg gcaaatgcac
    12301 aaacctacca acgtaatgcc gatacttata aagccaagat tacccaaacc cttgcccccc
    12361 tgcgtaagca gattgctgaa ctccctgaga atcagcgatg gatggtcacc agtgaagggg
    12421 ctttttctta tctcgcacgc gatttggggc taaaagagct ttatctgtgg ccgattaatg
    12481 ccgatcaaca aggcacgccg cagcaggtac gtaaggttgt tgatatggtg aagaaaaatc
    12541 atatcccggc agtctttagc gagagtacga tttccgataa accagcgcgt caggttgcgc
    12601 gtgaaaccgg cgcgcactac ggcggtgtac tctatgtcga ttccctgagc acagaaaacg
    12661 gcccggtacc tacgtatatc gaccttctga aggttactac cagtacgctg gtacagggaa
    12721 ttaaagccgg gaaaagggag aaataatgat gcaatctgcg ggcattgtcg ttaatgatgt
    12781 tactgtcacc tggcgtaacg ggcacacagc actgcgcgat gcatccttca cagtaccaag
    12841 tggatctatt gccgctctgg ttggggtaaa cggttccgga aaatcgacgt tgtttaaagc
    12901 gattatggga tttgtgcgtc tgaccagcgg aaagatatct gttctgggta ttcccacgcg
    12961 acaggcgcta cagaaaaacc tggttgccta cgtccctcag tcagaagagg ttgactggtc
    13021 atttcctgta ctagtggaag atgtggtgat gatgggccgc tatgggcaca tgggtatgtt
    13081 gcgtatagcc aaaaagaggg atcgtcagat tgtcaccgat gcgctggaac gtgtcgatat
    13141 ggtggatttt cgccatcgac aaatcggcga gctttccggt ggacaaaaga aacgcgtctt
    13201 tctggcgaga gcgattgcac agcagggtga tgtgatcctg ctggatgaac cttttaccgg
    13261 cgttgatgtg aaaaccgaag cgaaaatcat cagcctgttg agggagttac gcgcggaagg
    13321 caagacgatg cttgtctcaa cgcacaatct cggctcagtc acgacatttt gcgattatac
    13381 ggtcatggtc aaaggcaccg tcctggcaag cgggccgaca gacaccactt ttacagccga
    13441 gaacctggag ctggctttta gcggcgtact gcgccatgtc acacttaacg cctcggaaga
    13501 aagcattatt actgacgatg aacacccttt tgtggcacat cgaccgtcag cggtgcagag
    13561 ggaagcaaga tgaacgtgct actggaaccc ttcagctatg aatatatgct caatgcgatg
    13621 tgggtctcgg cgatggtcgg cggtctctgc gctttcctgt catgctatct gatgctaaaa
    13681 ggctggtcgc tgattggcga tgcgctgtcg cactctattg tcccgggtgt tgcgggggca
    13741 tatatgctgg ggcttccgtt ttctctggga gccttctttt ccggcggact ggcagcgggc
    13801 agcatgctgt ttcttaacca acgcactcgc ttaaaagaag atgccatcat cggcctgatc
    13861 ttctcctcct tttttggcct ggggctgttt atggtgtcat tgaacccgac atccgtgaac
    13921 attcagacca tcgtcctagg caatattctg gcaatcgatc cggcggatat cctgcaactc
    13981 actatcattg gcattctctc gatcatcgtg ctttttttca aatggaaaga tcttatggtg
    14041 acctttttcg acgagaatca cgcccgtgct attggattgc atccgggcag attaaaaatc
    14101 ctcttcttca ccctgctgtc ggtttcgacc gttgcggcgc tgcaaacggt tggtgccttt
    14161 ctggtgatct gtctggtggt gactcccggt gcaacagcat ggttgttaac cgatcgtttt
    14221 ccccgcctgc tcataattgc tgtaactatt ggcagtgtaa ccagtttcct cggcgcgtgg
    14281 gtcagttact ttctggacgg cgcgacaggt ggaatcatcg tggtggcgca gacactgctg
    14341 tttcttttag catttgtatt tgcgcccacc catgggcttt tggcaaatcg tcggcgtgcg
    14401 cataaagcgc tggaggatcg ctcatgatgg cattattact ggagccactg cagttcacct
    14461 ttatgagtca cgcactgctg atttcactgg tagtttccat accctgtgcg ctgctgtcgg
    14521 tctttctggt cctgaaaggt tgggcactga tgggtgacgc gatgagtcat gcggtttttc
    14581 ctggcatcgt gcttgcctgg attctggggt tacctctggc tacaggggca tttatcgccg
    14641 gagtattctg cgcggtggcg acgggatacc tgaaagacaa cagccgaatc aagcaagata
    14701 cagtgatggg gattgttttt tctggcatgt ttgccgcagg tctgatcttg tatattgcag
    14761 tcaaaccaga catacatctt gaccatattc ttttcggcga catgctcggg gtaaccatcg
    14821 gcgatataat ccagacgatg attattgccg ggctggttac acttgttatt agcgtaaaat
    14881 ggcgggattt tttgctgttc agctttgatt atcaacaggc gcaggtaagc ggtttgcata
    14941 cgcgatggct gcattatggg ctgctgtgca tggtttccct gaccattgtg gcgacgctga
    15001 aagcagtagg tatcattctc tccatttctc tgctcattgc gcctggcgcc attgcggtac
    15061 tcctgacaca acgctttcat attgcactcc tactggcgac tggcatatcg gtaatagtct
    15121 caatgactgg tgtttggctg tcctttttta tcgacagcgc accagctccg acgattgtcg
    15181 tcttattcgc ggttgtgttc atcatgacgt ttaccgttat cagtttcaac gcacgcacaa
    15241 agggaaacgc tcatagacag gatctgttat cccccaatta gcacaacatc cgagggcagc
    15301 attgtccgct agtacactgc agtccagaaa gtcatgggcg gacgttggga ggaggcgcta
    15361 atcctttcat acaaaaaata tgtaaaatca ataacggctg taaatcattc aatactcgca
    15421 ctatcggaag ttcaccagcc agccgcagca cgttcttgca tacgacgtgt ctgcggtttc
    15481 aacttcacca gtccaccaat cacgatggac agtgccaaga cagcactagc aaaaacagga
    15541 aattagcagt ctcagcagaa caccgaccag acggtgaaga aacaaaaaag gatacgcaaa
    15601 ggggccgcag ttcccgattg ataaaaaagc cagcttatgc gctttttttt catgtattat
    15661 taagtattgg ttagcgtctt tttaatcaga actttaaaga caagccattt gtaagaaaac
    15721 cgacagattc ggttgacaag caggagtaat actttggaca taagaaaaga tttggaatca
    15781 gtagcccaat atataagtag actgttatcg atagggtacg aatttagccg ttttgataaa
    15841 gactgggtcc atttaaaaaa tgaagaggat tttcgtttta tttcaagaat accatttgca
    15901 acacggaata aagtggaagc agtatatgcg gaaggcagag atatggcact ttatatgtct
    15961 gatgagttac tatccattaa tagtgatttc agtaaatttc caacgttgac agctattatc
    16021 gaaagattca aggacacttg ggtttatggt aattatgatt cggaagtgcc aaatatagct
    16081 aaaaaaacat gcgaagaaaa tgcagttcag ctttggtctg tagaacagat gtgttcctta
    16141 ttcaaaaagc aagaacaact attagcagca gtacgtatca cattgcagat gctacaagat
    16201 agtgacctat acaagatgga gaatggttta cctcttatga aacaagaagc taatattcat
    16261 gtcagtggaa tttccggttc tagtatcaat atacattctt caggtgctac ggcaactact
    16321 acgacaaatt ataatgagcc cacaatattt aatgaaatga ttgaggcaat aaaatcgaaa
    16381 aactttgatg gcgcgacaga atcacacctt atagataatg tacaggcact ggcagccagt
    16441 caccaaagcg gctgttttaa agaagcatat aaggacttta ttaataatgt atctgcacac
    16501 attacagtat ttactccatt tcttgctggc atatcatctt tactataaaa taagtgacag
    16561 atacattaat acataacgaa aaatcttttt agctactcat tcatatcaat agtgccctac
    16621 catagggcac attattcgtg gaactcgttt atgacaaaag aattttgatg gtctgcctga
    16681 tgcaacagat ataattctgg gagtgttgag taagagtatt caccggcgct caacacaaat
    16741 gccagcaagc acgagaaaga gcaggtaagc ctgagtgttc aggaaatgtt tgaaaaggct
    16801 aatatgtggc tggtttcaga ttaaacgcct tgaaccgtca tattacttaa gtacaatccg
    16861 cctagactag caatcattca atagtagcac tatcgagcgc tcgccagtcg agcgcaatca
    16921 tgctccttgc atacggcgtg cagcatatca caaaacgatt acgccatgac ggactgcagg
    16981 ctactcaata tctggctcga ttagcgtatt ggcatgattt aacagacccc ggcgcttatt
    17041 cccagcaacc tgaagcggca cctcttcctg tgttgcgttt acaactccct ttttcaccca
    17101 cgattaacca acagccagac cagcagacaa gccaccaccg gcacagaaaa atccatcagg
    17161 cttgccacat cccacacgcg cggatcaaaa ccaccccacc acggcatatt catacgcttg
    17221 ccatgcccga acatttcaat ccagcgatat tctgcctggg tatgttcacg agcaatgaag
    17281 aacgtacaac cggctatcgc cccgt
//
LOCUS       DAPOZO010000096        17171 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPOZO010000096 DAPOZO010000000
VERSION     DAPOZO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.096"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..540)
                     /locus_tag="ROI21_003674"
     CDS             complement(<1..540)
                     /locus_tag="ROI21_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5112549.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAP"
     gene            676..2400
                     /gene="iucA"
                     /locus_tag="ROI21_003675"
     CDS             676..2400
                     /gene="iucA"
                     /locus_tag="ROI21_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HDX5112550.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            2401..3348
                     /gene="iucB"
                     /locus_tag="ROI21_003676"
     CDS             2401..3348
                     /gene="iucB"
                     /locus_tag="ROI21_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HDX5112551.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            3348..5090
                     /gene="iucC"
                     /locus_tag="ROI21_003677"
     CDS             3348..5090
                     /gene="iucC"
                     /locus_tag="ROI21_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HDX5112552.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            5087..6424
                     /gene="iucD"
                     /locus_tag="ROI21_003678"
     CDS             5087..6424
                     /gene="iucD"
                     /locus_tag="ROI21_003678"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HDX5112553.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            6430..8625
                     /gene="iutA"
                     /locus_tag="ROI21_003679"
     CDS             6430..8625
                     /gene="iutA"
                     /locus_tag="ROI21_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HDX5112554.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(9183..9326)
                     /locus_tag="ROI21_003680"
     CDS             complement(9183..9326)
                     /locus_tag="ROI21_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112555.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            9576..13463
                     /gene="sat"
                     /locus_tag="ROI21_003681"
     CDS             9576..13463
                     /gene="sat"
                     /locus_tag="ROI21_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HDX5112556.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSKLKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTANHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNVYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLDNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVNNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
     gene            13510..14091
                     /locus_tag="ROI21_003682"
     CDS             13510..14091
                     /locus_tag="ROI21_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112557.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILDHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYSIIKGKVSSVLLLLKMLQINHA"
     gene            14429..14863
                     /locus_tag="ROI21_003683"
     CDS             14429..14863
                     /locus_tag="ROI21_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDX5112558.1"
                     /translation="MDKPTDWRSGTRRIFSNEFKLHMVELASKPNANVAQLAREHGVD
                     NNLIFKWLRLWQREGRISRRMPPTIVGPTVSQSFPASPTLVPVELIDTPRCATDAPAP
                     EALSVACAASCHVEFHYGKMMLENPSPELLTVLIRELTGRGR"
     gene            14860..15210
                     /gene="tnpB"
                     /locus_tag="ROI21_003684"
     CDS             14860..15210
                     /gene="tnpB"
                     /locus_tag="ROI21_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDX5112559.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQIQHVLDDNPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            15241..16853
                     /locus_tag="ROI21_003685"
                     /pseudo
     CDS             15241..16853
                     /locus_tag="ROI21_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc43 family transposase"
     gene            complement(16884..17140)
                     /locus_tag="ROI21_003686"
                     /pseudo
     CDS             complement(16884..17140)
                     /locus_tag="ROI21_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4541 a         4144 c         4294 g         4192 t
ORIGIN      
        1 tggcgccaga ggatgaatgg ccagcattgc tgccgcgcac agcggcccga atagcctgcc
       61 gcaactcagg ccggagctga tggttgccag agcggccatg cgattccctt ctcctgctct
      121 ctgcaatgcc cagacctgac aggctggcac cattgccgac acggtcagcc cgtaggcgat
      181 ccgggcgatg attaatattc ccagtcccac cgctgttgtt atggcgctgg tagccatcag
      241 cacgcttccc agccctagca gggtaaagct tatgccatat cctcccagag cccacaacac
      301 cacaggttta ctgccagtac gggaaatttg ctttccccac cacggggacg atggcagaaa
      361 cagcattgag ccaagcatca gcagggcagc ccatacagac agactcagat ttgtctgtat
      421 gaccagaaca gggagcacaa ccagcagacc gttctgcccg ataccgagaa gcccggcact
      481 gaacgcaagt ggccagcagg acagtggttt ttggggcgta tcttcgatcc caggtgacat
      541 tttaatgttt caactccatg tattaattgt gtttatttgt aaaattaatt tatctgacaa
      601 taacatttct tattgataat gagaatcatt attgacataa ttgttattat tttactgtgt
      661 gggagctgtt tgactatgac cctgccctct gaaaaaccag ccacagatgt ggctgcgcag
      721 tgcttcctga atgcactgat tcgtgaaacc acagactgga aactgacaga atacccgcca
      781 gacgaattga tcatcccgct ggatgagcag aaatcgctcc atttcagagt ggcttatttc
      841 tccccaaccc agcatcaccg ctttgcattt cctgcacgtc tggtcacggc atcaggcagt
      901 tatcctgtcg actttaccac tctctcccgg cttattattg ataagctacg gcatcaactg
      961 tttctgcccg ttcccctctg cgaaactttc caccagcgcg tgctggaaag ccacgcccat
     1021 acgcaacagg caattgatgc ccgtcatgac tggaccgccc tgcgtgaaaa agcgttgaat
     1081 tttggcgaag ctgagcaggc gctgctgaca ggacacgctt tccaccctgc gcctaagtct
     1141 catgaaccgt ttaaccggcg ggaggctgaa cgctacctgc ctgatatggc accccacttc
     1201 ccactgcggt ggttttcggt ggataaaacg caaatcgccg gtgaaagttt gcatctcaac
     1261 cttcaacagc ggctgacgcg atttgccgca gagaatgcgc ctcagttact caacgaatta
     1321 agtgacaacc aatggctgtt cccgttgcac ccgtggcagg gggaatatct tttgcagcag
     1381 gggtggtgcc aggcacttgt tgctaaaggg ctgattaaag acttaggtga ggccggcacg
     1441 tcgtggctgc cgaccacctc ttcccgttcc ctctactgtg ccaccagccg cgatatgatc
     1501 aagttctccc tgagcgtacg gctgaccaac tccatccgta ctctgtccgt gaaagaagtg
     1561 aagcgtggga tgcgcctggc acgcctggct caaaccgacg gctggcagat gctacaggtc
     1621 cgcttcccga ctttccgggt aatgcaggag gatggctggg ccgggctgct cgatcttaac
     1681 ggcaacatca tgcaggaaag tctgtttgcc ctgcgtgaaa atctgctggt ggatcagccg
     1741 aaaagccaga ccaacgtact ggtctccctg actcaggccg cacctgatgg cggtgattcg
     1801 ctgctggttt cggcggtaaa acgcctgagc gatcgcctcg gtatcactgt gcaacaggcc
     1861 gcccatgcat gggtcgatgc atactgtcag caagtgctaa agccgctgtt tacggctgaa
     1921 gcggattacg gcctggtgct gctggcgcat cagcaaaata ttcttgtcca gatgcttggg
     1981 gatctgccgg tcggatttat ttaccgtgac tgtcagggca gcgcttttat gcctcacgcg
     2041 acagactggc tcgattctat tggcgaggcg caggcggaaa atattttcac ccatgagcag
     2101 ttgctgcgct atttccctta ttacctgctg gttaactcca cttttgctgt gaccgctgcg
     2161 ctgggggctg ccgggctgga cagcgaatcg aatctgatgg ctcgtgtacg agcatcgctg
     2221 gctgaagtgc gtgatcaggt gactcataaa acctgtctca actacgtgct ggaaagtccg
     2281 tactggaacg taaaaggtaa ctttttctgt tatctgaacg atcataacga gaacaccatt
     2341 gttgaccctt cggtgatcta tttcgatttc gctaacccgc tgcaggctca ggaggtctga
     2401 atgtctgagg caaacattat tcacagcaga tatggactgc gctgtgaaaa actcgacaag
     2461 cccctgaatc ttggctgggg actggacaat agcgcggtgt tgcactgtcc cggggagctg
     2521 ccgacagggt ggctgtgcga tgcgctggat cagatattta tcgccgcacc acaactgtca
     2581 gcagtggctc tcccctgggc cgaatggtgt gaggagccac aggcgctgac gctttttggg
     2641 caggtaaaaa gcgacattat ccatcgtacc gccttctggc agttaccgtt atggttgagt
     2701 tcaccggcaa accgggcctc cggtgaaatg gtttttgatg cagagcgtga gatttatttc
     2761 ccgcagcgcc caccccgtcc gcagggtgag gtttaccgcc gttacgaccc acgtattcgc
     2821 aggatgctga gtttccgcat tgccgatccc gtttctgatg cagaacgttt cacccgctgg
     2881 atgaatgatc cgcgcgttga gtatttctgg gagcaaagtg gctcactgga ggtacagacc
     2941 gcctatctgg agcgccagct caccggtaaa catgcattcc cgctgattgg gtgcttcgac
     3001 gatcggccat tcagctattt tgaaatctac tgggcggcgg aagaccgcat tggtcgtcac
     3061 tattcgtggc agccctttga ccgtggcctg catctgctgg ttggtgaaca gcaatggcgc
     3121 ggggcccact atgtgcaaag ctggctgcgc gggctgacac attacctgct gctggatgag
     3181 ccccgtacgc agcgcacagt actggagcca cgcaccgata accagcgcct gttccgccat
     3241 cttgagcctg cgggataccg gacaattaaa gagttcgact tcccacataa gcgctcgcgc
     3301 atggtgatgg cggatcgtca tcacttcttc acggaggtcg gtctgtaatg aatcacaagg
     3361 actgggattt tgttaaccgt cggctggtgg caaaaatgtt gtctgagatg gagtatgagc
     3421 aggttttcca cgcggaatct cagggcgatg accactactg cattaacctg ccgggagcac
     3481 aatggcgctt tatcgctgaa cgtggtatct ggggctggct gtggattgat gcccaaacgt
     3541 tgcgctgcac ggacgaacca gtactggcgc agacgctgct gatgcagcta aagccggtgc
     3601 tgtcaatgag tgatgccact gttgcagagc atatgcagga tttgtatgcc acgctactgg
     3661 gcgacctgca actgctgaag gcccgtcgcg gactgagtgc cagtgacctg attaatcttg
     3721 atgccgatcg tctgcaatgc ctgctgagcg gtcatcctaa attcgttttt aataaaggtc
     3781 gccgtggctg gggcaaagag gcgctggaac gatatgcgcc agagtatgcc aacaccttca
     3841 gactgcactg gctggcggta aaacgtgaac atatgatctg gcgctgcgac aacgatctgg
     3901 atattcagca gttgttgact gccgcaatgg atccgcagga gtttacacgc ttcagtcagg
     3961 tctggcagga aaacgggctg gatcacaact ggctgccgct gccggtacat ccgtggcagt
     4021 ggcagcaaaa aatcgccacc gacttcatcg ctgattttgc cgaaggcagg atggtgtctc
     4081 tcggcgagtt tggcgaccag tggctcgccc agcagtcgct gcgtaccctg accaacgcca
     4141 gccgccgggg agggctggat atcaagctgc cgctgaccat ctacaacacc tcatgctacc
     4201 gggggatccc tggcagatat atcgctgccg gaccactggc ttcacgctgg ctgcaacagg
     4261 tttttgcgac cgacgccacc ctggtgcaaa gcggcgcagt gatccttggt gaaccggctg
     4321 caggctatgt gtcccatgaa ggctatgccg cgcttgcccg ggctccctat cgctaccagg
     4381 aaatgcttgg tgttatctgg cgggagaatc cgtgccgctg gctgaaaccg gatgaaagtc
     4441 cggttctgat ggcaacactg atggagtgcg acgaaaacaa tcagccgctg gcaggcgcat
     4501 atatcgaccg ctccggactg gacgctgaaa cctggcttac gcaattgttc cgggtggttg
     4561 tggttccgct gtatcacctg ctttgccgct acggtgtcgc gcttattgca cacggacaaa
     4621 atataacgct cgccatgaaa gagggggtac cacagcgtgt tctgctgaaa gacttccagg
     4681 gcgatatgcg gctggtgaaa gaagcgtttc ccgaaatgga ctctttgcct caggaggtgc
     4741 gtgatgtcac atcccgtctg agtgcggact acttaatcca tgatttgcag acgggtcact
     4801 tcgtgacagt actgcgtttt atttcgccac tgatggttcg tcttggcgta cctgaaaggc
     4861 gattttatca actgctggca gcagtgttga gtgattacat gaacaaacat ccacaaatgt
     4921 cagagcgttt tgcgcttttc tcactcttca ggccacaaat cattcgcgtg gtgctgaacc
     4981 cggtaaaact gacctggccg gaccaggatg gcggcagtcg catgctgccg aattaccttg
     5041 agaatctgca aaatccgctg tggctggtaa ctcaggaata tgaatcatga aaaaaagtgt
     5101 cgattttatt ggtgtcggga cagggccatt taatctcagt attgctgcat tgtcacatca
     5161 gatcgaagaa ctgaactgtc tcttctttga cgaacatcct catttttcct ggcatccggg
     5221 tatgctggta ccggattgtc atatgcagac tgtctttctg aaagatctgg tcagtgcagt
     5281 tgcacctaca aatccctaca gctttgttaa ctatctggtg aagcacaaaa agttctatcg
     5341 cttccttaca agcagactac gtacagtatc ccgtgaagag ttttctgact atctccgctg
     5401 ggctgctgaa gatatgaata acctgtattt cagtcatacc gttgaaaata ttgatttcga
     5461 caaaaaaagt cgattgtttc tggttcagac cagtcgggga gaatattttg cccgcaatat
     5521 ctgccttggt acaggaaaac aaccttattt accaccctgt gtgaagcatg tgacacaatc
     5581 ctgtttccat gccagtgaaa tgaatcttcg tcggccggac ctgagtggaa aacggataac
     5641 tgtggttggt ggaggacaga gtggtgcaga cctgttcctt aatgcattac gcggggaatg
     5701 gggagaagcg gcggaaataa actgggtctc acggcgtaat aattttaacg cactggatga
     5761 ggctgctttt gctgatgagt attttacacc tgaatatatt tcaggtttct ccggactgaa
     5821 ggaagatatt cgccatcagt tactggatga gcagaaaatg acatcggatg gcatcactgc
     5881 cgattcttta ctgaccattt atcgtgagtt gtaccaccgt tttgaagttc tgagaaaacc
     5941 aagaaatatc cgtctgctac ccagccgctc ggtaacaact ctggaaagta gtggtccggg
     6001 ctggaagtta ttgatggaac atcatctgga tcggggcaga gagagcctgg aaagtgatgt
     6061 ggtgattttc gccacaggtt accgttctgc attgccacaa atacttccct cactgatgcc
     6121 cttgatcacc atgcacgata agaacacctt taaagtgcgt gatgacttca ctctggaatg
     6181 gagtggcccg aaagaaaata acatctttgc ggtcaacgcc agcatgcaaa ctcatggcat
     6241 cgccgaaccc cagctcagcc tgatggcctg gagatctgca cgtattctta atcgcgtact
     6301 ggggcgtgat ttattcgatc tcagtatgcc gcccgcactg attcagtggc gcagcggcag
     6361 ccggaaaaaa ccgcagccgg aggctgcttc tttaactcac tatacagcaa atattcagga
     6421 ataacgatga tgcgcaaaaa gtatatgccc cgggctcttg gtccgctgct tcttgtcgtg
     6481 ctgtcaccag ctgtcgccca gcaaaacgat gataatgaga tcatagtgtc tgccagccgc
     6541 agcaatcgaa ctgtagcgga gatggcgcaa accacctggg ttatcgaaaa tgccgaactg
     6601 gagcagcaga ttcagggcgg taaagagctg aaagacgcac tggctcagtt aatccccggc
     6661 cttgatgtca gcagccagag tcgaaccaac tacggtatga acatgcgtgg ccgtccactg
     6721 gttgtcctga ttgacggtgt gcgcctcaac tcttcacgtt ccgacagccg acaactggac
     6781 tctgtcgatc cttttaatat cgaccatatt gaagtgatct ccggcgcgac ggccctgtac
     6841 ggtggcggga gtaccggagg gttgatcaac atcgtgacca aaaaaggtca gccggaaacc
     6901 atgatggagt ttgaggctgg cacaaaaagt ggctttaaca gcagtaaaga tcacgatgag
     6961 cgcattgccg gtgctgtctc cggcggaaat gaccatatct ccgggcgtct ttccgtggca
     7021 tatcagaaat ttggcggctg gtttgacggt aacggcgatg ccaccctgct tgataacacc
     7081 cagaccggcc tgcagcactc caatcggctg gacatcatgg gaaccggtac gctgaacatc
     7141 gatgaatccc ggcagcttca actgataacg cagtactata aaagtcaggg ggacgacaat
     7201 tacgggctta atctcgggaa aggcttttcc gccatcagcg ggagcagcac accatacgtc
     7261 agtaaggggc tgaattctga ccgcattccc ggcactgagc ggcatttgat cagcctgcag
     7321 tactctgaca gtgatttcct gggacaggaa ctggtcggtc aggtttacta ccgcgatgag
     7381 tcgttgcggt actacccgtt cccgacggta aatgcgaata aacaggcgac ggctttctcc
     7441 tcgtcacagc aggataccga ccagtacggc atgaaactga ctctgaacag ccaactgatg
     7501 gacggctggc aaatcacctg ggggctggat gctgagcatg agcgctttac ctccaaccag
     7561 atgttcttcg atctggctca ggcaagtgct tccggagggc tgaacaacca taagatttac
     7621 accaccgggc gctatccgtc atatgacatc accaatctgg cggccttcct gcaatccagc
     7681 tatgacatta atgatatttt taccgttagc ggtggcgtac gctatcagta tactgagaac
     7741 agggtagatg atttcatcga ctacacgcag caacagaaga ttgctgccgg gaaggcgata
     7801 tctgccgacg ccattcctgg tggttcggta gattacgata actttctgtt caatgctggt
     7861 ctgctgatgc acatcaccga acgtcagcag gcatggttca atttttccca gggggtggca
     7921 ttgccggatc cggggaaata ttatggtcgc ggcatctatg gtgcagcagt gaacggccat
     7981 cttcccctga caaagagcgt gaacgtcagc gacagtaagc tggaaggcgt gaaagtcgat
     8041 tcttatgaac tgggctggcg ctttaccggt gacaacctgc ggactcaaat cgcggcatat
     8101 tactcgcttt ccaataagag cgtggaaagg aataaagatc tgaccatcag tgtgaaggac
     8161 gacaggcgcc gtatttacgg cgtggaaggt gcggtggact acctgatccc ggatactgac
     8221 tggagtaccg gtgtgaactt caatgtgctg aaaaccgagt cgaaagtgaa cggtcaatgg
     8281 caaaaatatg acgtgaagga atcaagtcca tcgaaagcga cagcttacat taactgggcg
     8341 ccggaaccgt ggagtctgcg tgtacagagc accacttctt tcgacgtaag cgatgcagag
     8401 ggtaacgata ttaatggtta cactaccgtc gattttatca gtagttggca gcttccggtg
     8461 ggaacactca gcttcagcgt tgagaacctc ttcgaccgtg actataccac tgtctgggga
     8521 cagcgtgcac ctctgtacta cagcccgggt tacggccctg cttcactgta cgactacaaa
     8581 ggccggggcc gaacctttgg tctgaactac tcagtgctgt tctgacgggg atgcttttgc
     8641 aacaagggta tgctgtaaac aacatggtcc ttggtggcca tgttgtttac agccagtatt
     8701 gtttacagcc agtataatat acagcctaac aagatattcc gtctccggaa tggactgttc
     8761 accactgaat aacattatgt ctgttttatg gatctttgac tgaaatttat cgcatatcct
     8821 catttcattg tccgtctgaa aactaacctt acaagtaagc gtcgtctcag caccgtctgg
     8881 caaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg ctgcaatgaa
     8941 ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa aaagaggtac
     9001 ctgtcgctgg ttctccgttt agtttaaccg ggagattttc tcctgcgcct gtcataaagt
     9061 ccggtaatgg agattgttaa tacgatggtg cacgatgcat tgccagtgcc cggcccggag
     9121 gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg ctataggcaa
     9181 agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt ctgaataacg
     9241 cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat cgctaattcg
     9301 tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg aaatattaaa
     9361 cattacccct ccccatattg ggcaaaaaaa aacaaaaaac gatcaattaa cgatcaatta
     9421 gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt tacaatatag
     9481 aagtgttact tttagcaagc tgttagtaac caactgttac taataatttt aattcttaat
     9541 actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac tcccttaaat
     9601 atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga gtttctggta
     9661 aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt acagtaaatg
     9721 cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt gcacaaaata
     9781 aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac ggagataaat
     9841 tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt ggcgcagcta
     9901 ccgcaatagg aggttcttat agtgttactg ttgcacataa caaaaagaac cctcaggccg
     9961 cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga aattccaatg
    10021 attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc accccggcag
    10081 agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc acttctgacg
    10141 gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt attaatggtg
    10201 aatccaaaat ctcaacaaat tcagcatata gccatgatct gttaagtgct agtctatttg
    10261 aggtcaccca atgggactca tacggcatga tgatttataa aaatgataaa acatttcgta
    10321 atcttgaaat attcggagac agcggctctg gagcatactt atatgataac aaactagaaa
    10381 aatgggtatt agtcggaaca acccatggta ttgccagcgt taatggtgac caactgacat
    10441 ggataacaaa atacaatgat aaactggtta gtaagttaaa agatacctat agccataaaa
    10501 taaatctgaa tggcaataat gtaaccatta aaaacacaga tataacatta caccaaaaca
    10561 atgcagatac cactggtact caagaaaaaa taactaaaga caaagatatt gtgttcacaa
    10621 atgggggaaa tgtcctgttt aaggataatt tggattttgg tagcggtggt attatctttg
    10681 acgaaggcca tgaatataac ataaacggtc agggatttac atttaaagga gcaggaattg
    10741 atatcggaaa agaaagcatt gtaaactgga atgcattgta ttccagtgat gatgttttac
    10801 acaaaatagg ccccggtact ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag
    10861 gtgaaggaaa tgttattctt aatgaagaag gaacatttaa caatatatac cttgcaagcg
    10921 gaaatggtaa ggtaatacta aataaagata attcccttgg caatgatcaa tatgcgggga
    10981 tattttttac taaacgtggt ggcacgctag atttaaatgg acacaatcag acttttacta
    11041 gaattgccgc cactgacgat ggaacaacaa taactaactc agatacaacg aaagaagccg
    11101 ttctggcaat caataacgaa gactcctaca tatatcatgg gaacataaat ggcaatataa
    11161 aactaacgca caatattaat tctcaggata agaaaactaa tgcaaaatta attctggatg
    11221 gtagtgtcaa cacaaaaaat gatgttgaag tcagtaatgc cagtcttacc atgcaaggcc
    11281 atgcaacaga gcatgcaata ttcagaagca cagcgaatca ttgctccctg gtatttcttt
    11341 gtggaacgga ctgggtcacc gttttgaaag aaacagagag ttcatataat aaaaagttca
    11401 attctgatta caaaagtaat aatcagcaga cctcatttga tcagcctgac tggaaaaccg
    11461 gggtgtttaa atttgataca ttacacctga acaatgctga cttttcaata tcacgcaatg
    11521 ccaatgttga aggaaatata tcagcaaata aatcagctat cacaatcggc gataaaaatg
    11581 tttacattga taatcttgca gggaaaaata ttactaataa tggttttgac ttcaaacaaa
    11641 ctatcagtac taatctatcc ataggagaaa ctaaatttac aggtggcatc actgcacata
    11701 acagccaaat agccataggt gatcaagctg tagttacact taatggtgca acctttctgg
    11761 ataatactcc tataagtata gataaaggag caaaagttat agcacaaaat tccatgttca
    11821 caacaaaagg tattgatatc tccggtgaac tgactatgat gggaatccct gaacagaata
    11881 gtaaaactgt aacgccgggt ctccactacg ctgctgatgg attcaggctg agtggtggaa
    11941 atgcaaattt cattgccaga aatatggcat ctgtcaccgg aaatatttat gctgatgatg
    12001 cagcaaccat tactctggga cagcctgaaa ctgaaacacc gactatatcg tctgcttatc
    12061 aggcatgggc agagactctt ttgtatggct ttgataccgc ctatcgaggc gcaataacag
    12121 cccccaaagc tacagttagc atgaataatg cgatctggca tctaaatagc cagtcatcaa
    12181 ttaatcgtct agaaacaaaa gacagtatgg tgcgttttac tggtgataat gggaagttta
    12241 caacccttac agtgaacaac cttactatag atgacagtgc atttgtgctg cgtgcaaatc
    12301 tggcccaagc agatcagctt gttgtcaata aatcgttgtc tggtaaaaac aaccttctgt
    12361 tagtcgactt cattgagaaa aatggaaaca gcaacggact gaatatcgat ctggtcagcg
    12421 caccaaaagg aactgcagta gatgtcttta aagctacgac tcggagtatt ggcttcagtg
    12481 atgtaacacc ggttatcgag caaaagaacg atacagacaa agcaacatgg actctgatcg
    12541 gctataaatc tgtggccaac gccgatgcgg ctaaaaaggc aacattactg atgtcaggcg
    12601 gctataaagc cttccttgct gaggtcaaca accttaacaa acgtatgggt gatctgcgtg
    12661 acattaacgg tgagtccggt gcatgggccc gaatcatgag cggaaccggg tctgccggcg
    12721 gtggattcag tgacaactac acccacgttc aggtcggtgc ggataacaaa catgaactcg
    12781 atggccttga cctcttcacc ggggtgacca tgacctatac cgacagccat gcaggcagtg
    12841 atgccttcag tggtgaaacg aagtctgtgg gtgccggtct ctatgcctct gccatgtttg
    12901 agtccggagc atatatcgac ctcatcggta agtacgttca ccatgacaac gagtataccg
    12961 caactttcgc cggccttggc accagagact acagctccca ctcctggtat gccggtgcgg
    13021 aagtcggtta ccgttaccat gtaactgact ctgcatggat tgagccgcag gcggaacttg
    13081 tttacggtgc tgtatccggg aaacagttct cctggaagga ccagggaatg aacctcacca
    13141 tgaaggataa ggactttaat ccgctgattg ggcgtaccgg tgttgatgtg ggtaaatcct
    13201 tctccggtaa ggactggaaa gtcacagccc gcgccggcct tggctaccag tttgacctgt
    13261 ttgccaacgg tgaaaccgta ctgcgtgatg cgtccggtga gaaacgtatc aaaggtgaaa
    13321 aagacggtcg tatgctcatg aatgttggtc tcaacgccga aattcgcgat aatcttcgct
    13381 tcggtcttga gtttgagaaa tcggcatttg gtaaatacaa cgtggataac gcgatcaacg
    13441 ccaacttccg ttactctttc tgataacagc ccgggccgcg tttgcggccc ttcttctacc
    13501 ggagagaata tgtattaccc tgtgacagac tatatcgctc ttgctctcat tattagcttt
    13561 ctttttctga cattatttat ctgcctgtta tgccttaaac atgagcgaat aaaaaaagaa
    13621 actatcaggc aaaaaaatgc acatattctg gatcatggct ggaatgcaac tgagttctca
    13681 tggttccgat acggacagta taacgaaacg ggtatttaca tctcgatcga gaagaccatt
    13741 atcattacaa tcaccgtttc tggtgggtgc tttaaaaaag aatacagcat tgtctcccac
    13801 atgctggtca ctgacaccat aacagaggcc accctgtacg aaaacgggct gtatacgcgc
    13861 cacatcagac tcagtcgccc agtatctgac agcaaatacc ccttgcctcc cggatctcag
    13921 ctcattaaaa atatgacctt acgactgcgc ctgcaggatc agcaggaaga aacatccgtg
    13981 acattatttc agggaaaaat gagtaccgac ggaaataact attccatcat caaaggtaaa
    14041 gtaagctcag tacttctgtt attaaaaatg ctgcagatta atcacgcctg atctctgtcc
    14101 cggtatttta gcccgaggta tattctgaaa tatcattcat catgaccggc gtttatacaa
    14161 acgccggtca ttcgctaaca gcttcaccgt tccttccccc atccaggttc cgcatgagcg
    14221 tgttgtaatg ttcataagcg tattatcagg attgtcgtaa gcgtaaactg accgccgtat
    14281 gtagccatta agccagtatt ggtaacgtag gtgcccacct tttcatcaac tagtggacac
    14341 ctggtaagcg taaagccaat gccgtctgta acacctgctc cttgcagact aaattagagc
    14401 tccttctaaa ttagacggag ttctattgat ggataaacct acagactggc gctccggaac
    14461 ccgccggata ttttctaatg aatttaaact tcatatggtt gaactggctt cgaaaccaaa
    14521 tgccaatgtt gcacaactgg cccgggaaca tggcgttgat aacaacctga tttttaaatg
    14581 gctacgcctc tggcaaagag aaggacgtat ttctcgtaga atgcctccaa ctattgtagg
    14641 ccctacagta tcacaatctt ttccggcctc tccgactctg gttccagtgg aacttatcga
    14701 caccccgcgc tgtgctacag atgctcctgc tccggaggca ttatcagttg cttgtgcagc
    14761 ttcctgccat gtggaattcc attacggtaa aatgatgctg gaaaatcctt caccagagct
    14821 gctcacggtg ttgatccgtg agctgaccgg gaggggacga tgatttcact cccatcaggt
    14881 acccgtatct ggctcgttgc cggcgttact gatatgcgta aatccttcaa cggtctgggg
    14941 gagcagatac agcatgtgct ggatgataac cccttctccg gtcacctgtt tatcttccgt
    15001 ggccgacggg gagacacgat taaaatcctg tgggctgatg ctgatggtct gtgcctgttc
    15061 accaaacgcc ttgaggaagg tcagtttatc tggcctgcgg tgcgtgacgg taagatatcc
    15121 attacccgct cgcaactggc aatgctcctc gataagctgg actggcgtca gccaaaaaca
    15181 tcccgcctta acgcactgac aatgttgtaa aaaactcctg accgcattat aaaaacggcc
    15241 atgagtcaga aatacctcat tcgcatcgct gagctggaaa ggctgctctc tgagcaggct
    15301 gaagccctcc gtcagaaaga ccagcaactg agtctggttg aagagacgga ggccttcctg
    15361 cgctctgcac tggcacgtgc cgaagaaaag atcgaagaag atgaacggga aatagagcat
    15421 ctgcgggctc agatagaaaa actgcgccgg atgctgttcg gaacccgttc tgaaaaactg
    15481 cgtcgtgagg ttgaacaggc tgaagccctg ctgaaacaac gcgagcagga aagcgatcgt
    15541 tacagtgggc gtgaggatga cccgctggtt ccccgccagt tgcgacagtc acgccatcgt
    15601 cgtccgttac cggcatatct cccccgtgaa atataccgcc tggagcctga agaaagctgt
    15661 tgcccggagt gtggcggtga gctggattat ctgggggaag tcagcgcaga acaactggaa
    15721 ctggtgagca gcgccctgaa agtgatccgc acagaacggg taaaaaaagc ctgtacaaaa
    15781 tgtgactgca tcgttgaagc accggcacca tcccgtccga tagagcgtgg tatcgcgggc
    15841 ccggggttac ttgcccgcgt gttaacggga aaatactgcg aacacctgcc actgtatcgt
    15901 cagagtgaaa tctttgcccg ccagggtgtc gaactgagcc gtgccttact ctccaactgg
    15961 gttgatgcgt gctgccagtt aatgactccg ctgaatgatg ccctgtacag ttatgtgatg
    16021 aacacccgca aggttcacac tgatgacaca ccagtaaaag tactggcacc gggcaggaag
    16081 aaggcgaaaa caggatatat ctggacgtat gtccgggatg accggaatgc cggttcgcca
    16141 gaacctccgg cggtctggtt cgcctactca ccggaccatc agggtaaaca tccggagcaa
    16201 caccttcgtc ccttccgggg tatcctgcag gcagatgcgt tcgcaggtta cgatcggctg
    16261 ttcagtgccg aacgtgaagg cggcgcgttg acggaagcag gatgctgggc tcatgcgcgg
    16321 cgcaaaatcc acgatgtata tatcagtact aaaagcgcga cggcggaaga agcactgaaa
    16381 ctaatcggcg aactgtacgc cattgagcac gaaatacgcg ggttgccggt gtctgaacgc
    16441 ctggcggtca ggcaaatgca gagtaaaccg ctactgactt ccctgtataa gctgatgcag
    16501 gagaaagaac acacgttatc gaaaaaatgc cgtctgagag atgcgttccg gtatatcagg
    16561 aagcactggg ttgcgttgtg caacttctgt gatgacggtc tggcggaggc ggacaataac
    16621 acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga aaaattcgtg ttcttcggta
    16681 gcgatcacgg cggcgagcgt ggtgcactgt tgtacgggct gatcggcacc tgccggttga
    16741 atggtatcga tccggaagcg tatctgcgcc atatcctgag cgtactgccg gaatggcctt
    16801 ccaaccgagt tgacgaactc ctgccatgga acgtagtact cactaataaa taagcgtcaa
    16861 tacggtgctc cgttgacgct tacacgcacc tgcttttcca gctcacgaat acattgctgc
    16921 tctgatgtga atggtgctgc agaaggcgtg atctcctgac gttcccgtct gagctgacat
    16981 acccatgcct ccagagtaga gaaaccaaca ttcatagctt cagtagcttc ccggtatgag
    17041 taacctttat caacgataag ttgggcgcat tccagattaa actcaggaga gaaggtacgc
    17101 tttgttttct tgttgtttta tgttgtggtg agaatatcac tagactggcc ccctgaatct
    17161 ccagacaacc a
//
LOCUS       DAPOZO010000097        17104 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPOZO010000097 DAPOZO010000000
VERSION     DAPOZO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.097"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..455)
                     /locus_tag="ROI21_003687"
     CDS             complement(<1..455)
                     /locus_tag="ROI21_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HDX5112560.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSS"
     gene            complement(585..1328)
                     /gene="traF"
                     /locus_tag="ROI21_003688"
     CDS             complement(585..1328)
                     /gene="traF"
                     /locus_tag="ROI21_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HDX5112561.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKISPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEYYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPKF"
     gene            complement(1321..1578)
                     /gene="trbE"
                     /locus_tag="ROI21_003689"
     CDS             complement(1321..1578)
                     /gene="trbE"
                     /locus_tag="ROI21_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HDX5112562.1"
                     /translation="MKVIFTSNRLVDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTGLQKADERKNDERNEDE"
     gene            complement(1605..3455)
                     /gene="traN"
                     /locus_tag="ROI21_003690"
     CDS             complement(1605..3455)
                     /gene="traN"
                     /locus_tag="ROI21_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HDX5112563.1"
                     /translation="MKRILPLILALVTGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCSRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPVAGTVESASLSVHAGLYFL
                     NSRYDFMNTTFNVQLVTGQSGTFTIPVPSGFSVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLKGTECSEPGGTKT
                     GVMEGKPWSITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESKTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(3452..4090)
                     /gene="trbC"
                     /locus_tag="ROI21_003691"
     CDS             complement(3452..4090)
                     /gene="trbC"
                     /locus_tag="ROI21_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HDX5112564.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTTENRQFLKQQETLSQQL
                     REKPDIQLQAWAEQQVRANPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(4099..4404)
                     /locus_tag="ROI21_003692"
     CDS             complement(4099..4404)
                     /locus_tag="ROI21_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112565.1"
                     /translation="MATMDSKYIQHLRKIGAVLLRLISRTLNLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCVFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(4434..5426)
                     /locus_tag="ROI21_003693"
     CDS             complement(4434..5426)
                     /locus_tag="ROI21_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HDX5112566.1"
                     /translation="MKRRLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(5423..6055)
                     /gene="traW"
                     /locus_tag="ROI21_003694"
     CDS             complement(5423..6055)
                     /gene="traW"
                     /locus_tag="ROI21_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HDX5112567.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRIYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(6052..6438)
                     /gene="trbI"
                     /locus_tag="ROI21_003695"
     CDS             complement(6052..6438)
                     /gene="trbI"
                     /locus_tag="ROI21_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HDX5112568.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(6435..9062)
                     /gene="traC"
                     /locus_tag="ROI21_003696"
     CDS             complement(6435..9062)
                     /gene="traC"
                     /locus_tag="ROI21_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HDX5112569.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLKS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYRV
                     FFSYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNYKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAARDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(9222..9443)
                     /gene="traR"
                     /locus_tag="ROI21_003697"
     CDS             complement(9222..9443)
                     /gene="traR"
                     /locus_tag="ROI21_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HDX5112570.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQEKQRKHYA"
     gene            complement(9578..10093)
                     /gene="traV"
                     /locus_tag="ROI21_003698"
     CDS             complement(9578..10093)
                     /gene="traV"
                     /locus_tag="ROI21_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HDX5112571.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKELEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATPPSGSRPAVTALPEQKLLAPR
                     PLFTAAREVKTIVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(10093..10452)
                     /locus_tag="ROI21_003699"
     CDS             complement(10093..10452)
                     /locus_tag="ROI21_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HDX5112572.1"
                     /translation="MFISLMTGQTETTEIKMNMRNIITARSVPDKSVSDDFMNAVLSN
                     CTTRIVLPALKEFGSESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGQPCAGKTNL
                     TVELISAVVESNKKGEG"
     gene            complement(10391..10978)
                     /locus_tag="ROI21_003700"
     CDS             complement(10391..10978)
                     /locus_tag="ROI21_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HDX5112573.1"
                     /translation="MPARRAEMAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMAL
                     FVLWKDNTTLGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAAD
                     AADYVRETDRSLMQLVTAFWATLALMYVSLAAITGKYPVRPGKMKCVRVGTADEHLKE
                     VYTEDTSLSEKIRKCHVYFPDDRANRNNGDKNEHA"
     gene            complement(10947..12398)
                     /locus_tag="ROI21_003701"
     CDS             complement(10947..12398)
                     /locus_tag="ROI21_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HDX5112574.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVMYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKNGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGDKNPQGGDAGKEGGNGG"
     gene            complement(12398..13102)
                     /gene="traK"
                     /locus_tag="ROI21_003702"
     CDS             complement(12398..13102)
                     /gene="traK"
                     /locus_tag="ROI21_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDX5112575.1"
                     /translation="MFGSLFFSCSVMAANGTLSPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGACMDVYVIRDGEGN"
     gene            complement(13113..13679)
                     /gene="traE"
                     /locus_tag="ROI21_003703"
     CDS             complement(13113..13679)
                     /gene="traE"
                     /locus_tag="ROI21_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HDX5112576.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLKYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(13701..14012)
                     /gene="traL"
                     /locus_tag="ROI21_003704"
     CDS             complement(13701..14012)
                     /gene="traL"
                     /locus_tag="ROI21_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HDX5112577.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKRRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWV
                     K"
     gene            complement(14017..14379)
                     /gene="traA"
                     /locus_tag="ROI21_003705"
     CDS             complement(14017..14379)
                     /gene="traA"
                     /locus_tag="ROI21_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HDX5112578.1"
                     /translation="MDAFLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGLK"
     gene            complement(14413..14640)
                     /gene="traY"
                     /locus_tag="ROI21_003706"
     CDS             complement(14413..14640)
                     /gene="traY"
                     /locus_tag="ROI21_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HDX5112579.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVIEENESTFKEL"
     gene            complement(14728..15405)
                     /locus_tag="ROI21_003707"
     CDS             complement(14728..15405)
                     /locus_tag="ROI21_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDX5112580.1"
                     /translation="MRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKKRKILYANGA
                     FIELFSKEDKPFSGESYVRLQVEIFLSSLELECQSLGHGSAFCRRFNFHGEIYQIRME
                     NVSFYNEESVVLWQINIFPDYPFFRVEKENYYHRDSYVQSVISNMTAKSLVVFCFYAL
                     GYKHINIAKELKITEVASKKRIKKINADINKRLGSFDLFRNKCIKTGVMYEILNVLSE
                     YMGVKKM"
     gene            complement(15539..15922)
                     /locus_tag="ROI21_003708"
     CDS             complement(15539..15922)
                     /locus_tag="ROI21_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HDX5112581.1"
                     /translation="MAKVQAYVSDEIVYKINKIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVYETQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPENDEG"
     gene            16253..16855
                     /locus_tag="ROI21_003709"
     CDS             16253..16855
                     /locus_tag="ROI21_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDX5112582.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
BASE COUNT         4152 a         4626 c         3974 g         4352 t
ORIGIN      
        1 gagctgggtg cctcaatgat ggtggcatcc accaaagtgc cttgggtcat catgacgcct
       61 gcttcggcca gccagcgatt gatggtcttg aacaattgac gggccagttg atgctgctcg
      121 agcaggtggc ggaaattcat gatggtggtg cgatccggca gggcgctatc cagggataat
      181 cgggcaaaca ggcgcatgga ggcgatttcg tacagggcat cttccatggc accgtcgctc
      241 aggttgtacc aatgctgcat gcagtgaata cgcagcatgg tctccagcgg atagggccgt
      301 cggccattgc ccgccttggg ataaaacggc tcgatgacag cggtcatatt ctgccatggc
      361 aaaatctgct ccatgcggga gaggaaaatc tcttttcggg tctgacggcg cttagtgctg
      421 aattcactat cggcgaaggt gagttgatgg ctcatgatgt ccctctggga tgcgctccgg
      481 atgaatatga tgatctcata tcaggaactt gttcgcacct tccttaacag aacaacacct
      541 gtaaacattt tcaaataatc ttcactcata aatcaaatcc gcgattaaaa ttttggttta
      601 aaatcttcag aaacgttcag gaattgtttt gccaggtcgt cctgcgaaat aaagccgtat
      661 gataacgggc gaacactgcc ctgtttcggg tcaaccagca tcatggccgg gaaatatttc
      721 acgccgaggc gctgcgcctg cccctggtca gtccgggaat ccggcaacag cggattaatc
      781 acgccatcca cggaaacggg aataacactc agaccatacg tatcccggaa gccattaatg
      841 acctgcgcca gctgcccgtc gatggggtcc tgcccccggt aaaaaaacat gatgccgtag
      901 tattcagcca gtttcgcaat ggcctgtcgc tgctgcgcct ggtctgccgc cagctggttc
      961 cgtaccgtgc cgttgtaatg gctgtactgc aggttatagt ccagttcggg atgtgccagc
     1021 atggcctttt tagcactcat ggtgaaaagc ccggcctgct gagtccagta attctgcagc
     1081 cggaaatatt tcacaaaatt atccacgccg ggatacagaa tggcttcgta cagcgcccgc
     1141 tttgttgccg tctgcagcgc ggccagtttc tgcataatat ccggctcctg acggggggct
     1201 gcaggtacag gtttattttc tttttccttt ggacttattt tctcgttata ccactgccag
     1261 cctgcatctt tccctgacgc cggaaaaata aagcagcaga gtaacagtgg cagtaatgct
     1321 ttattcatct tcattccttt catcattttt tctttcatca gctttctgca ggccagtcag
     1381 ggcagaaaaa caaaaataca gaataaacag gagcacaccg gagtaaagga taacgaaagc
     1441 agcggcaagc atcccgtctg ccgtggtttc ttttaccgta tcccacgacc agataattac
     1501 cggagaaatg acaatcgctg taatcaaaag ccggataaga aaatcaacaa gacgattaga
     1561 agtgaatatg actttcatca tttcccccgg ttattttcca tttcttactg tcctgcctgt
     1621 ttcagctggt cagcaatctg ctctttcact ttctgcgtca gaacgccact gtccgggatt
     1681 ttctggttat tcatcaggtc ttcgtagaag ttagtgaaat ccagtctgtc gaactgaatt
     1741 ttctgcagct catcaaccgt aatcccccga cagtcaggat gcttcgcact gccaaacccg
     1801 atacgcaact gcccgttacg cccctgctgc tggactatct gcgccagctt cgaatcaaac
     1861 tggcagtagc tgcgcttttt ctgcaggcag acccccagca cctttttcga acagaactca
     1921 cccacactca ccgtcagttt attgtccttt gcctttccca gcgccttttc ttcactgttg
     1981 cacttcgcca gccccacatc ctgaccccag ccgccatcct tacagcagtt actgaagccg
     2041 gctgcggcct tacggcagaa tttcgcctca cccgtgaaag ccctgacatc cacaccattc
     2101 agggcggcaa catccttacc tgcggcggca agcgcagcca gctgtgatac tgcttcacca
     2161 aaatcactgc tttttccgct ctgtgcctta tcgcattcac cgtcaaggca gaagacatca
     2221 ccaccacaga ccatcacttt tcctgatgtt tttgactcgc aggagtacgt ggcgtactca
     2281 tgcagacagg ttccctcatc ggaataaaac gcgcactgac gtgaggccag cgtacaggcc
     2341 ggattatcga catatttctg acaggtgccg ttatctgcgg actgagtgac atatttatcc
     2401 cggtatgccc agcatgcctg tgtgatgctc cagggtttac cttccatcac acccgttttc
     2461 gtaccacccg gctctgaaca ctcagtgcct ttcagcacgc catccgcttt attaaacgga
     2521 caactctcca cccactccac ccggggaacc cattctttat cacggacctt catcctgagt
     2581 ttcagcgtaa aggtggaagc accactgaca agcgattcat agactttcct gtcaccattc
     2641 ccgtgcggga ggcaattacc gtttgcagta cagccactac cggtcagaac ctgcccctgt
     2701 gtgacagaaa acccggaggg cacaggtatg gtgaaagtcc cgctctgtcc tgtaaccagt
     2761 tgcacattaa aggttgtgtt cataaagtcg taacgggagt taaggaaata tagccctgca
     2821 tgaaccgata gcgaggcact ttccactgtc ccggcaacag gtacggtaac gctgaacacc
     2881 agtttcagac cgttcatact gaaccggaac tgtgagtgcg gtatcgtcac ctcacgatat
     2941 tcatccgtgt acttccagtc tccggtgata ctggcagtcc gggagcagta ttcctccacc
     3001 atcgtgtccc gctcacaggt atagtttgtg aactcactgc ggtttatctg ctgcgcactg
     3061 cactgctgcc cggtgtttcc cacaatgctg tcagcccggt tcaccacatc cctgccggtc
     3121 tggataaacg gcgcatccgg tgaaagaatg tctttcggct tgttcatgaa tgactctgtg
     3181 atagtttttc cggtttcacc ggtcgcccat tccgtggtgc cgtcattttt caggccgcca
     3241 tccccgccgg cagtcacgcc accgtaatat tttgtctcgt ccggattcgc gttatagccg
     3301 gggatactct cctgtggctt aaagccctga atactgctgc ttccctgccc tttgatctgg
     3361 tgagcaaaat cagaaccggc ccggtaatca ctgttgctgt cagcctgtgc cataccggta
     3421 accagagcca gtatcagagg taaaatacgt ttcatttccc ggaatctcct ttccctgcca
     3481 gtaaatcatg agccacctgc cggcagtcac ccgtggcggc cactttctcc agcgcctggc
     3541 cgacccgcag atttccccgg atgatgtcgt atccctgact gcagaacacc accagtgccg
     3601 gtacgctgcg aatgccgtac tgtgaaaaca gcgtcgggtc gatctgaaca ccatcagttg
     3661 cgccgtcttt caccagggac aacacggctt cagcggtggt cttcaggtca ttgttcacca
     3721 tgccccgcag tgtggccgga ataccgtagt gccgggtttc gcccagcatc cgtttcagcc
     3781 cctcttcggg aatggagaag gacacaaagt acacggcgcc ctgctgtggt ttatccttct
     3841 gagcagcctg ctgtttacgg accagttcat cgagaaactg gttatcggaa tgctgaaggg
     3901 gattcgccct gacctgctgc tctgcccatg cctgcaactg tatgtccggc ttttcacgta
     3961 actgctgact cagtgtttcc tgctgcttca ggaactggcg gttttcagtt gtgttcacat
     4021 tttctgaagc atgaacagca agatgcatcc ctgctgtcag agcaaaaagt gcaaatattt
     4081 tccggttcat gatatttctc actttctgct atttacaggc atgaactcat cagaaaaaag
     4141 ataattgaga aaaacagcca taaacacaca tgtaaatgtc accttcagat ataatctgac
     4201 aagtgtcact tgttctgata tatctgcctt cttccattca tgaataattt cagcgagaaa
     4261 actgtcaggt gcaaaataaa ataatataac aggcaccatt acaagaccaa aaggaaataa
     4321 atttaatgtt ctgcttatca gcctgagcag cacagcacct attttcctga ggtgctgaat
     4381 atatttactg tccattgttg ccattttcat ttcctcttat tcattaaaat gatttacagg
     4441 aatacacagt tacgtttacg ccacatcagg tagccgaagt ttttctttgt gttgggcgga
     4501 ttttttcccg tctcccagcg catcacactg cgcccgaacg gatggcactg tccgctgtcc
     4561 ggatacatat tcaccatctg ataacgccag cgttctttgg gcaggattgg ggacggatat
     4621 tcattacaga cggcgttatt tttcccgatg gtttccataa tcatgccctg acggtgcagc
     4681 ttgaatgcca tgcgttcact gaccagcagg gaggactgca acggactgga ctcattactc
     4741 acccagccat tgaacgggta catactgccc tgcgaaccgg cacaccagaa cagaacatcg
     4801 agaggcatat taaaggcact ggcaatggca tctgctgcgc aggccccctg tgctatcgga
     4861 ttggcaaaaa tgacggcttc cgggttgaga atggtggtca ggctgctgtc cgtccaggtg
     4921 gggtcgattt cagaaagata agcgatatcc aggtcaccac cttccagaca gcccagcgat
     4981 gtgatgatgt tcagccagta cgtcagcggg tatttgtacc agtgaacgtg atagaacgcc
     5041 ccgttcacct gcttctcatc ctttttcgcc gttccctgtg ccgttttacc aaaagccggc
     5101 aggctgaagc ccaggttcac catgcatccc ggtgaccggg tgacgtccgt cagcgccatc
     5161 ggctcccagt aaccaatggc cagcccgata cgcctgaaca gcggcggcgg tgccggacaa
     5221 atctgaatag gcatcgacgg gttcgccgtg tcggggacct taccctggct gactttgata
     5281 ctgcccagcg agagcgggaa aatacagctc cagcagatat ccgtgatcgg gtttacaaaa
     5341 cgcccctcac aggcagaatc cgcagacgcc gcagggacat gaccggcgag aaggaataac
     5401 atcagcagcc acagtcttcg cttcattttc tgccctcctc tgccggaata aattccacct
     5461 tcaggaagcg atcgccggga acggcgctca cccgtgccgg gacctgatca atgccgaggc
     5521 gctggcagag cacaccgttc tggtcaaagt aaatacggct gtccagcgct ttctgcatct
     5581 ccgggatact gccctgcacg aggataattt tgctctccag tgtgggcggc gtctggcgtt
     5641 tcatccaggc cacctgcgcc ggatcatcgc cattgatgaa atacagcgtc tggttaaaag
     5701 gaacatactg cagcggattc atcacttcac cctgacgggc aaacacccgc ccttcgttat
     5761 cccggatatc cgcagccagt ctgacggacg ggtcaaacaa ccggctgctg tatttctccg
     5821 tgcgtccgat accgggcacg gcagggggcc gcagactgtt acggatcacc cgctccttaa
     5881 aggcatccat tttccggccc atctcaccgg actgctccag tgccgtcagg cgctgcatga
     5941 tcaccgtcag catgtccggc tcctttaccg gccacagatc gccccaggtg ccaagatcgg
     6001 cggcggccac actctgcccc catatcagca gggcaatcag cccccggcat ctcatggttc
     6061 cgctctcatt cgccgggcaa tatcctgctg gatttcccgg gtgatatccg gagccccctg
     6121 taccacggca ggcgacacca gaatgaccgc gtggtgtttc tgctgccacg cctgcaggct
     6181 ggcttccagt gccgtgttaa aacgcgctga aagggctttt gactgtgctt ctgacagttg
     6241 tttctgactg gcgctgtcaa aaaatgcatc cacagtctgt ttcatgttga aggcggcagt
     6301 caccggtgcg ttcagtctga caatgccgta actgacggct gcgttcagta acaccattgc
     6361 caggcatccc ggcaccgtcc accaccagtg gctgcgtcgg ggggctgtaa catcagtcgt
     6421 tttttgcgtt gtggtcatgc aacactcctg tatttctcat gttcttccag ccaggcttcc
     6481 agcgaagcca tctccggccc tgacttcttc cacgccagct gccacaccgc ctcatgaata
     6541 ctcagcccct ctttacgttt ctgctgcaca aactcaaaat ccgggccgtc agagctgtac
     6601 atggcgcgac ttaacgggtc cacaaacagg cggtgccagg aggaatggtt ttccacctgc
     6661 agcaggaagg aactgaacca ctggtctctg gccgcaccaa acttaccaat catgtcgcgc
     6721 tgcagtggct ggaactggtc cggatacagc tggttgtatt ttgcgaactc cttcgcactc
     6781 tgtttgagga taattttgta ggaggagtta ccccatgccg cacgggccgc actggaggcc
     6841 ttgtcagagt caaagtcgac gatgttctgt gtgatggtga tataagcacc ggtatgacga
     6901 cgggcggtac ggtaaccttt ctcaataaac tcaccgacct tgtaattttt gaagtccagc
     6961 agacgccagc cttcatcaat gacgttcagt ttcttgaggt tacgcggcgt gcggtacatc
     7021 ctgttctcga tgtagataat cagggagaac atcaccgcaa ccagcagtga cggacggtct
     7081 tccagtccgc ccagctccag caccaccatt tttgcgtcat cccgcaggga cggctcatca
     7141 gagttaaaat actgaccgta agtgccgttg gcagtgtact ggtcaagcag cacaatcatt
     7201 tcgtccagac ggctgcggat agtcggtgac ccggcatact gctcgctgtc actggcgttt
     7261 ttcaggaaat ccaccacgtc atcaatacgt gccctgttct ctttggccag ccaggaggcc
     7321 ctgaccgcct gcagcagcag accttcatgc acttcatcca ggttaccgtt ggggctggcc
     7381 atcactgaca actggtcacg gacacgctcc gctgactggt cgatatcggt gatattcgca
     7441 aacgggttaa aacgcagggt ttcaccgtcc agatatacgc cacccatgtt ctcacacagg
     7501 gacttgtagc catcccccat gtcgaacacc acggcaaagc ccccggagtc cagcacgctg
     7561 cggataaggg gctgtatcag tccggtttta ccggccccgg aggtgccaca gaccgccatg
     7621 ttgtagttgg tgttattcat cccccggaag aaaatatcga taaacgccag ctggttacgg
     7681 taggtgggtg ccagcagacc tgccggtgtc agggggttat ccgccactaa cggcatcagg
     7741 ttggcaacat taaagctctc tgcgcgctga accacaccgg cctctttcag ctgtttaaac
     7801 agccctttcc cggccataaa gggcaggcag gtcaggaaat tgcgcatgtg gttaaagcgc
     7861 ggtgaaatca gctcaaaacc gtttttacgg aagctgttca ggatgtcctg ctccacttcc
     7921 agtgccgttt cattattgtc cttgcagaag gctgtgatgt tgaggaagta ggacacgaca
     7981 gaagactgac cggagcccag ccgctgacgc agttcccccc actccttcgc ctctttctcc
     8041 acggacggaa accatttggc ataggaggtc ttcgactttt tctccaggtc catgtacttc
     8101 aggttggctt cgctgtgggt tttcacctgg tcttccacca ccagagtcag cgtcaggatg
     8161 aacggacagg agatggacag ttccgggttc agcaggttgc tgtagttgtc ggccatattc
     8221 cacaggaagg cgatttccgg gttacgggcc agatggaaat tcaggatgcg ggccgtgctg
     8281 ttcctgccgt tctcacgcag gcccagcgtc aggtaatcag cccggacttt caggtcaaaa
     8341 ctgtcctcca cacactgata attcagatca gaatacgggt ccagctgacg tcttttcggg
     8401 tacagggagt ccgggttatg attaatcatc tccccgacaa tatcgataaa ggcctgtgca
     8461 tccaccgtct gtgtggcgat actggccccc tgtaacgacg cccggatgat tttcaccagg
     8521 ttttccattt ccagaatgtc agcccggctt tttttcttcg agggagaaca gtacgagaag
     8581 aatacccggt aatggcgcag ggtcaggggc agattcatcc cctccggcag cggaaactgt
     8641 gtcgctgccg cgttcatata ataggcacgg gtaatggcgt taaaccgttc agcctgttca
     8701 ccggaccagg agaactcacg cagcccgtat tcaattctgt caccaacaag ctggctggac
     8761 ttcagatgga tgcagaacgg cactccgcgt ggcagtttgg tgcgcagcat gtggtcgagc
     8821 gcctccacaa tggactcatt cgccccattg atgggaatgg cttccagcat aaagcccatc
     8881 gtggtgtcat tcatgaacag accggactcc tggttgtaat cacggtacgg cagcagacgg
     8941 ctgaactgcg ggaagctcat ttcgcccaga acttcattgg cctttgcgga ttcgtcaggc
     9001 agtttcagtg ctgtgacgag ggagttaacc gcctgagtga cggcctcaag tgggttattc
     9061 acatgaattc cttagttgaa aaataaagcg agaaatgcgg tagccatgaa gggaatacct
     9121 gtaattaacg cgccatttcc cggcgcatat acaaagattt cggttttctg aatatcaact
     9181 catgttcatc cggaattaaa tcaatatgtt ctgcgactga cttatgcata atgttttctc
     9241 tgcttttcct gatacgcctg acattcaacg cacaacgtca caccgggaaa tattttccgc
     9301 cgggcttccg gaataggatt tccgcatgct tcacagagat aaacaggaat accatgaaca
     9361 tttattttct ggcgtatccg gtttattcct gtcatggtca gttgttctgt cactgaatat
     9421 gcttcatcgg cttcatcact cacggaatat tcctcccgac atgttctgac acgctaccgc
     9481 ccatcccttt tcacgcacag ggcactcgtt ttacaggcag attttgaata cagaaagccc
     9541 gccggcattg ccggctgata tacaacttca gggccagtta attaatacgt ggttttcccc
     9601 acgcagacgg tttaataaca aaaaacacac tggatggctg atgataaaca tcctgattat
     9661 caatataagg cgctatccat aatgcagccg tctgctcacc cgttcttagc gggcggggag
     9721 gtgttaccgg cgcaactgaa ctgaccggaa caatcgtttt cacttcccgg gcagccgtaa
     9781 acaacggacg tggtgccagc agtttctgct ccggaagtgc cgtcaccgca ggtctgctgc
     9841 cggacggagg ggtcgcggtg acggtctgca ccggcattgt ccggaagttc ccttcagcca
     9901 ggcgcggcag tgatgccgca accggctttg cttctgagga ctgctccagt tctttggcct
     9961 tctcattggc ctgctccatc gtcatacagg tatcggatgt ggtggcgtta cactcaaatt
    10021 ccgtactggt tccggcacag ccggaaagta ataacgttcc cagcagagga ataaataaag
    10081 aaatctgttt cattaccctt ctcctttttt attactctcc acaacagcag aaattaactc
    10141 tactgtcaga tttgtttttc ctgcacaggg ttgtccggag actactgtat ttcccggaga
    10201 agaaaataag ctcatcgaca tacccttctt catcacaccg acagctgcca tattaaaatt
    10261 gtgcggcaat gattcagaac cgaattcctt cagtgcagga agtacgattc ttgtggtgca
    10321 gttactgaga acagcattca taaaatcatc tgatacgctt ttatccggta cagagcgtgc
    10381 agtaataata ttacgcatgt tcatttttat ctccgttgtt tctgtttgcc ctgtcatcag
    10441 ggaaataaac atggcatttc ctgatttttt ccgacagaga agtatcttcc gtataaactt
    10501 ccttcagatg ttcatctgcg gttcccaccc ggacacattt catttttccg ggtcgcaccg
    10561 gatatttccc ggtgatcgct gccagcgaga catacatcag tgccagtgtt gcccagaatg
    10621 cggtgaccag ttgcatcagt gaccggtcag tctccctgac ataatctgca gcatcagccg
    10681 cgcggtaatg gcagatatcc tgtatttctg atctgcctga agaatcagag gaagaaaacc
    10741 agcattcact gaccgggaac tgtccggcag gtgctgtttg tcttacgaag ttaatctcct
    10801 taaccagtaa tttccccagc gtggtgttgt ccttccataa aacaaacaga gccatcaggg
    10861 ccattgtggc cagcggccag ataaccacaa acctgacgac ccacagcagc cagcgaacga
    10921 caaacgccac acggaatgtc agtgcatcac ccgccatttc cgccctcctt gccggcatcc
    10981 ccgccctgcg ggttcttgtc tccggacata aactgctgcg gattcagatt gcccagctgc
    11041 ttcagcatct ggtcggtgtt aaaaccgttc aggtggcttt ctgccgaggg aggattcggc
    11101 acagggcttt ccgggttacc ctcttccgct gtactctgtg actgctccag cgccatttcc
    11161 tccacggttt tcagctggaa gccgtcctgg aacaccactg tcacttcgtt gcccgcacca
    11221 atcggtatca ccgggtgata ctgttcggca cgtttgatgt agtagtcact gagcgtctgt
    11281 gcggctttcg atgcgccgcc accgataccc attttaagga catcgccggc cccgtaagcg
    11341 gctgtggcac ccagcccgac agccggctgg gaggcgcgct ccattccctg accgataccg
    11401 tcaacaaatc ccgcgcccca tgcccagccg aggattttgc cgttacgcat caccacttcg
    11461 cccttgatac cgtttttacc ccggaagctg acatgcccct taatcggcat atcaatggtt
    11521 ttgccattct tcaggcagct gatattacgg gtgcgaacaa tagcacgttc actggagaca
    11581 tccccccagg cttccagccc cacaaaacat cccgttgcgt catacgtctt gctgttcggc
    11641 atttccacca gaccggtgat acgcagctgc atcggcaccg tggattcatt accggtgact
    11701 gaggcattgg catccgcccc ttcaatcagc atggctttcg caaaacttcc tgacggaatg
    11761 tacggcagcg atggtccctg ttttccctca ttacgggtaa acaccttacg ctgtatccgg
    11821 ttaggcaccg gcacagactg gtacatcacc tgtggtggtg gcgtgacacc attcccggga
    11881 taaaacgccg tcggcggtgg aacagcaacg ctgccctgcg gcgggaagga tacgggggtg
    11941 tttcccggct gtggttcgcc ttccggcccg ggcggtgagg ctggcgtctg cggtacaggc
    12001 tcaccggtcg ccgtgacggg attagcaccc agggctttta cctgctctgc cagggcggca
    12061 ttgtcctgtc ccagtttttc aatacgacgc tggtcatcac cgcgctgttt gttcaggaca
    12121 tccagttcac gacggatttc ctcatactgc ttctgcatct gcgctgccgt cacctgcatc
    12181 tctgtggtgg catgctgacg caccttgtca tcaaaggtcg tatccaccac accggtcata
    12241 tccggcacag gctcctgttc agccacggtt tccccgttac cggacatgtc cacatcagac
    12301 agatacagtg ccccaccaat cgcggaggct gtaccgacaa ccacaatccc cagccacagg
    12361 tactgcttgc gtttcacaat ggtgttgata ctggccatca gttgccctcc ccgtcacgga
    12421 tgacatacac atccatgcac gcacctgcca gtaactggct ggcaggctgg ctgaacatca
    12481 cggcacgggt tcccggctgc cagaagtcac tttcccggat attcagggca gacagcgttt
    12541 tattttccac ggcaaagcgg accatcttca ggtgattccc cgtccatacg gcatctgcca
    12601 ctgaagacag ccccgccggg gcctgcaggg tttcctttgt cacaggaacc tgataccagc
    12661 ctgcaggtaa ttttccgcca cggacagcct ggctgatggt cacaagcagg gattcgtaag
    12721 gcgtggacgt ttcccacgca ccggcttctt ctccggttcc gcgcaggtca ctgaccagct
    12781 gaatggtacg ccccgcgcct tcacggggaa cggcctgaat ggaaagattc agaccacgtt
    12841 ctgtttccag aatgaacgta aagggctttt tgttgacggt ggcaaccacc acaccgccgg
    12901 aggcggtctg ctcattattg gtcagggcac catccagact gttcacggcg ataatccggt
    12961 caccgggaac ggtaaacaga ttcgggctgg tattgctgat ggcaatactg gcctgaccac
    13021 cgttcaccat tggcaccacg gtgggggaca gcgtaccgtt tgcggccatc acgctgcagg
    13081 aaaaaaacag gctgccgaat attattgccg tattattttt tctcatcgtc tgtttccccg
    13141 aaattatcca gccaggtcac cccgttttcc cgcttcagaa taaggatgta atgtttgata
    13201 tcagtgaaag gttttgaatc accaatccag gttttaagca caccacgaat ttccacacgg
    13261 ccatactgag gccagacacg aacactggtc tggaaaaagg ctgagttcac gttatcgttt
    13321 ttaatacgct tcgcttcttc agccagaata actttcatct ggttctgtgc gcccgggcgg
    13381 atatatttca gaagcgcctg atgtgaggca tcgacggttt ctgatgaaac attcagacgg
    13441 agggcaataa atgacagcgc catctgctgt aaataagagg cgtcggcact gttctgtgac
    13501 acggcaaagg gggcattaaa tgccattggc gtcacggcag tgcgttgctc attctgaaga
    13561 cggtagttat tcaccccctg aatgacgtta acagagaggc tgagaacaat aagcactgac
    13621 ataaatataa aggcgatggc cattacacga ctggtactta aacgggcacc gtgttccata
    13681 taacaatcct ggtatcagtt ctatttaacc cactgccgga aacacgaatc gggaacatta
    13741 tgaaaaatac cgcgcagcag ggctgttggc atataccagt aaatcaggtc tcgtaaccag
    13801 gaactgcccc gccttttttt cagtttttta atcccgaaat aaaccagaac cgctgcacca
    13861 ataccgaaca gatattttga tgttgtgata ccccagccaa tacagattgc ggcggggatc
    13921 agttcatcca gtggcaggcc aaaccagcgg ctctggttgg tcagtgtttc cgggaaacga
    13981 tatttcttaa gtttattctc gtctcccgac atcgttttat ttcaggccaa cgacggccat
    14041 acccacagca ataaatacag agatgatggc aaaaccggcc aggaacttga cgtttttggt
    14101 catcatgtac atgaccgcac cgaccagaac ttcagccaga acaacccatt taacaacact
    14161 ggagtcctta ccgaaggtcg ccttaaccgt ggtgttaccg cttgccatca ggtcctggcc
    14221 ctgtgctgcc attgccagtt gcgggaagaa catcatcaga acagcagcag ggatcacagc
    14281 gcgagccagg cgaagcatat tcagacgagt gaatttagaa aaaaacgatt tctttttgac
    14341 gggcgcagaa gcaccctgaa cacttaaaaa ggcgtccata tgattcctct gtttaatatt
    14401 gatagataaa aactaaagtt ctttaaatgt tgactcgttc tcttcgatca cctccctgaa
    14461 tatttcttca ttatagaaat caggaaaccg tttcagatga tctctgagac ggatctgaac
    14521 ctctatagtt ttacttctac cgctacgatc tttggcccga ataagtcggt tatttgtatc
    14581 ttcatcgaga tacactgaga ctgttctgct tatcccgcct ctcgcgttac gcctcctcac
    14641 tcaattcacc tctgtttcct gaccaataaa atcatgattg cacagaaatg gcctgtcgaa
    14701 aatgggccca aaacgagccc tgctttatta cattttttta acacccatat attcactcaa
    14761 aacatttaat atttcgtaca ttacacctgt cttaatacat ttattcctga acaaatcgaa
    14821 ggaacccaat cttttattga tatccgcatt aatcttttta atcctttttt tagaagccac
    14881 ttctgtgatt tttaattctt tagctatatt tatatgttta taccctaatg catagaaaca
    14941 aaaaacaacc aatgattttg ctgtcatatt cgatataaca gactgtacat agctatcacg
    15001 atgataataa ttttcttttt ccacacgaaa gaatggatag tcaggaaata tattaatttg
    15061 ccatagaaca acagattctt cattataaaa tgaaacattt tccatcctta tttgatagat
    15121 ttctccatga aaattaaaac gacgacaaaa tgcggaacca tgcccaagag actggcattc
    15181 aagctcaagt gatgaaagaa aaatttcaac ctgcagacgt acataacttt caccagagaa
    15241 gggtttatct tctttggaaa agagttcaat aaaggcccca ttggcataaa ggattttcct
    15301 ttttttgtta cggacacaaa caggaacagg agaattcctg tagatatttt gaacgttaag
    15361 aatgtattta tttacagatt gactgttaag tggcctttct ctcatcggcg atttttaata
    15421 tcaagtttta acgtggcatt aaccacataa ttaatataac ctttatgcgg ttaattgtca
    15481 tcaaattgaa ccagatcaaa atcctgttat ttgaagcaaa tcaactttta attctttttt
    15541 aaccctcatc attttctgga aagaacctct ccatctcaga tgacaccttc tccctgatat
    15601 cttcgaccat attcgcatat tcaaactttg gatttccgga gacatgagga ctgagagact
    15661 caatacccaa aattttcgct actgatgatt gtgtttttac aacgcattca agaagcaatt
    15721 tattaaactc agtctgatta aatgcagact ctttacgctc catctgagtc tcatatacac
    15781 gaagccctaa ttcgagaagc atagtggata tgcttgagaa acttacatct gtacttttag
    15841 ctccttctgc acgtcttctt tccacaattt tatttatttt atagacaatt tcgtcactga
    15901 cataagcctg cactttcgcc atatgtatac ctctttattt tcttatcgca tcaaatttat
    15961 ttcacatata aaaaaaagac aattcatcga tgaatcgaat cgtgacgcaa gttacgaaaa
    16021 ttacgattca tgcagcaaat cacatcacga attgaatcta gagtcaattc gctttaactc
    16081 gttgttttat tattactgat tcatctatga gttgtgttaa attcattggt gaatcatatg
    16141 cgattcacca atgaatcctt tttataatgt aaaataaatt aaaatacatt atttaaaaca
    16201 taagttaatg attcaagtag caaatcagca aaaacttgtt tttgcgtagt gtgtggtgct
    16261 tttggtggtg agaaccacca acctgttgag cctttttgtg gagtgggtta aattatttac
    16321 ggataaagtc accagaggtg gaaaaatgaa aaaatggatg ttagccatct gcctgatgtt
    16381 tataaatgag atctgccaag ccactgattg ctttgatctt gcaggccggg attacaaaat
    16441 agatcctgat ttactgagag caatatcatg gcaagaatcc cgttaccggg ttaatgccat
    16501 cggtattaat ccggtaacgg gatatggcag cggactgatg caggtagatt cccagcattt
    16561 taacgaactg gcccgctacg gaattaagcc agaacatctg acaacagatc cctgcatgaa
    16621 catttatacc ggtgcttatt atctggcaat agcatttaaa aaatggggcg tcacctggga
    16681 ggccgttggt gcatacaatg ccggattcag gaaaaccgaa cgccagaacc agagacgtct
    16741 tgcctacgca tcagatgttt accggattta taccgggata aaaagcagta aaggtatccg
    16801 gcttccggcc acgaagaaat cacttccgga aattaacagc gtgcagaaca attaatctta
    16861 cagacattgt cagataccgg ttatgccgca aaagcggcat ggaaggccac acacaaaaat
    16921 atcatatgag tgacaatact gactggccga agccccgcga agcgggaacc catcttatat
    16981 atacccggta actgcgcagc ctcctgccag accatttttc gcaggcacaa tatttacaga
    17041 caaccggaaa gccggttgat gaaattgagg tatatcagac gacacagcaa tgccgaaaaa
    17101 tgat
//
LOCUS       DAPOZO010000098        17064 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPOZO010000098 DAPOZO010000000
VERSION     DAPOZO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.098"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..867
                     /gene="ldtC"
                     /locus_tag="ROI21_003710"
     CDS             <1..867
                     /gene="ldtC"
                     /locus_tag="ROI21_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDX5112583.1"
                     /translation="LPPAGSRLVGENKFHVVENDGGSLEAIAKKYNVGFLALLQANPG
                     VDPYVPRAGSVLTIPLQTLLPDAPREGIVINIAELRLYYYPPGKNSVTVYPIGIGQLG
                     GDTLTPTMVTTVSDKRANPTWTPTANIRARYKAQGIELPAVVPAGPDNPMGHHAIRLA
                     AYGGVYLLHGTNADFGIGMRVSSGCIRLRDDDIKTLFSQVTPGTKVNIINTPIKVSAE
                     PNGARLVEVHQPLSEKIDDDPQLLPITLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPV
                     DVRRHQVSPQTL"
     gene            complement(950..1207)
                     /gene="bhsA"
                     /locus_tag="ROI21_003711"
     CDS             complement(950..1207)
                     /gene="bhsA"
                     /locus_tag="ROI21_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDX5112584.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            1448..2080
                     /gene="comR"
                     /locus_tag="ROI21_003712"
     CDS             1448..2080
                     /gene="comR"
                     /locus_tag="ROI21_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDX5112585.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AVANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(2142..2681)
                     /locus_tag="ROI21_003713"
     CDS             complement(2142..2681)
                     /locus_tag="ROI21_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDX5112586.1"
                     /translation="MNKSMLAGMGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(2908..4212)
                     /gene="ndh"
                     /locus_tag="ROI21_003714"
     CDS             complement(2908..4212)
                     /gene="ndh"
                     /locus_tag="ROI21_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDX5112587.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(4612..5154)
                     /locus_tag="ROI21_003715"
     CDS             complement(4612..5154)
                     /locus_tag="ROI21_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX5112588.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(5177..6202)
                     /gene="nagZ"
                     /locus_tag="ROI21_003716"
     CDS             complement(5177..6202)
                     /gene="nagZ"
                     /locus_tag="ROI21_003716"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDX5112589.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(6213..7037)
                     /gene="thiK"
                     /locus_tag="ROI21_003717"
     CDS             complement(6213..7037)
                     /gene="thiK"
                     /locus_tag="ROI21_003717"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDX5112590.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(7018..7659)
                     /gene="lpoB"
                     /locus_tag="ROI21_003718"
     CDS             complement(7018..7659)
                     /gene="lpoB"
                     /locus_tag="ROI21_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDX5112591.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(7673..8050)
                     /locus_tag="ROI21_003719"
     CDS             complement(7673..8050)
                     /locus_tag="ROI21_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDX5112592.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(8053..8412)
                     /gene="hinT"
                     /locus_tag="ROI21_003720"
     CDS             complement(8053..8412)
                     /gene="hinT"
                     /locus_tag="ROI21_003720"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDX5112593.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            8746..10935
                     /gene="fhuE"
                     /locus_tag="ROI21_003721"
     CDS             8746..10935
                     /gene="fhuE"
                     /locus_tag="ROI21_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDX5112594.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIVLALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KFRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVINPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLITTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(10995..12428)
                     /gene="ptsG"
                     /locus_tag="ROI21_003722"
     CDS             complement(10995..12428)
                     /gene="ptsG"
                     /locus_tag="ROI21_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDX5112595.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(12723..13520)
                     /locus_tag="ROI21_003723"
     CDS             complement(12723..13520)
                     /locus_tag="ROI21_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDX5112596.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(13531..14535)
                     /gene="holB"
                     /locus_tag="ROI21_003724"
     CDS             complement(13531..14535)
                     /gene="holB"
                     /locus_tag="ROI21_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDX5112597.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(14532..15173)
                     /locus_tag="ROI21_003725"
     CDS             complement(14532..15173)
                     /locus_tag="ROI21_003725"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDX5112598.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLESVMDAIRTTVTNWVKELDA"
     gene            complement(15163..16185)
                     /gene="yceG"
                     /locus_tag="ROI21_003726"
     CDS             complement(15163..16185)
                     /gene="yceG"
                     /locus_tag="ROI21_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDX5112599.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(16188..16997)
                     /gene="pabC"
                     /locus_tag="ROI21_003727"
     CDS             complement(16188..16997)
                     /gene="pabC"
                     /locus_tag="ROI21_003727"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDX5112600.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVIPVRACGDVSFSSATLYEYLAPLCERPN"
BASE COUNT         4284 a         4513 c         4161 g         4106 t
ORIGIN      
        1 ctgccgccag cgggcagtcg tctggttggc gaaaacaaat ttcatgtggt ggaaaatgac
       61 ggtggttctc tggaagccat cgccaaaaaa tataacgtcg gctttctcgc tctgttacag
      121 gctaaccccg gcgttgatcc ttacgtaccg cgcgcgggca gcgtgttaac gatcccgttg
      181 caaaccctac ttccagatgc gccgcgcgaa ggcattgtga tcaatattgc ggagctgcgt
      241 ctctattact acccgccggg taaaaattcg gtaaccgtgt atccaatagg tattggtcag
      301 ttgggtggtg acacgctgac accgacaatg gtgaccaccg tttcagacaa acgagcaaac
      361 ccaacctgga cgccaacggc aaacatccgc gcccgttata aagcacaggg aattgagttg
      421 cctgcggtag tgccggctgg accggataac ccaatgggcc atcatgcgat tcgtctggcg
      481 gcctatggcg gcgtttattt gcttcatggt acgaacgccg atttcggcat tggcatgcgg
      541 gtaagttctg gctgtattcg tctgcgggat gacgatatca aaacactctt tagccaggtc
      601 accccaggca ccaaagtgaa tatcatcaac actccgataa aagtctctgc cgaaccaaac
      661 ggtgcgcgtc tggttgaagt acatcagccg ctgtcagaga agattgatga cgatccgcag
      721 ctgctgccaa ttacgctgaa tagcgcaatg caatcattta aagatgcagc acaaactgac
      781 gctgaagtga tgcaacatgt gatggatgtc cgttccggga tgccggtgga tgtccgccgt
      841 catcaagtga gcccacaaac tctgtaaatc ctggataaaa aaaatccccg cgacacggca
      901 tgtcgcgggg aaaaaatcag cagtagcagg cattaatgag ggttaatgct tatttataaa
      961 ttactgctgt tccatggagg gtatttggac cggttacaga agtaatacgg aatgattttg
     1021 cgcccatctc atccgctttt tgcgccagct gctcttccag cgatcccaga tttgtccccg
     1081 cgttagcact gatagtaccg actttttgtt ggccttctgg cgttgactga acttcgacag
     1141 ccgcaaagct ggcaaatgac atggagctta aaatcgccgc agcgatgagg gtttttacgt
     1201 ttttcataat agtggcctta tgcagatgaa tgacgtcgca aacaattact taacgatcga
     1261 taactaaatg atagatgtga tctggatcac atacaagata ttttttataa caatcattaa
     1321 ttaatttaaa aatctttaaa tttcagttaa atagaaagca attattttta tggtttgtgg
     1381 actggtgaac ggaggtgatt atttttataa taacctttca atcacagtca ccaggtacaa
     1441 cggcatcatg gcaactgact caacacaatg tgtaaaaaaa agccgtggcc gcccaaaagt
     1501 gttcgacagg gatgccgcgc ttgataaggc catgaaattg ttctggcagc acggttatga
     1561 agcgacttct cttgcggacc tcgtcgaagc gaccggagcc aaagcgccca cgctatacgc
     1621 ggaatttacc aacaaagagg ggttatttcg cgccgtactc gaccgctata tcgatcgttt
     1681 tgccgctaag catgaagcac agctgttttg tgaagagaaa agcgtggagt ctgcgctggc
     1741 tgactatttt gctgccgtcg ccaactgctt taccagcaaa gacaccccgg ctggctgctt
     1801 tatgatcaac aactgcacca ccctctcccc ggattccgga gatatcgcca atacgctgaa
     1861 atcacgccat gcgatgcaag agcgcacttt gcagcagttt ttatgtcaac gacaagcgcg
     1921 cggggaaatc ccgacccact gtgacgtgac gcatctggct gaattcctta attgtattat
     1981 tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg gaaaaactga tgcagattgc
     2041 cggaacgact ttgcgtttat ggcccgaact ggtgaaataa gtcgcaaaaa aagcccctca
     2101 gcctgagcga atgaggggcc aaattgcaga gtacagcctt gttatacttt gttattcaaa
     2161 atcagttgcc cgttactatc tagcgggatc tgcgtacccg gatcgcgatc catgcggatt
     2221 ttgccctgct gatcgccaat cttataggtc acatcataac cgagcatttt ttctgacttg
     2281 tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag tatcgctttc ctggagagag
     2341 ccctggatct ggttaccggc atagccacct cccagcgccc ccacaacagt ggcgacatct
     2401 ttaccgcgac caccgccaaa ctgatgccct atcacgccgc cagcaacagc gccgagcacc
     2461 gacccggtaa tgcgattttc atcctgcacc ggtcgacgat gggtcactgt gacgttgcga
     2521 cactcctgac gcggtgtttt aaccgtttcc ttgattgggg ttgcagaaac aacctgagcg
     2581 tattgcgggc cacgttcaaa cacgttcaga ctggccactg ccgctacgcc caacgcagct
     2641 gcgacaccaa tccccatacc cgccaacatt gatttattca cgggaacacc tccttctttg
     2701 ctgttatgcc aatcatgcaa gcgaaggaga aaagcagcta tcagacagaa gatgaaaagc
     2761 gcgggaatgg ctggaaatac ttagggattc ggaggagtct gaaggaatgc ctgatgcgac
     2821 gcttaacgcg tcttatcagg cctaccgctc gtaacatttg tccagggcgg ataaggcgtt
     2881 tacgccgcat ccgccagtac aagccgatta atgcaacttc aaacgtggac ggataacgcg
     2941 gttaatactc cccaccagca tcattaatcc ggttttaaag taaccatgca gcgcaatctg
     3001 atgcattctg tatagcgaga tgtatacaaa gcgcgcaatt cgtccttcaa tcatcattga
     3061 gccgcgcgtc aggttaccca tcaggctacc gacggtggag aagttcgaca gcgataccag
     3121 agaaccgtga tctttatact gataattttt cagcggctta ccattcatct gcgccagaat
     3181 gttgttcatt gcgcaagttg ccatctggtg tgcagcctga gcgcgcggcg gaacaaagcc
     3241 cccttccgga cgcgggcatg acgcgcaatc gccaatagcg taaatgtctg gatcgcgagt
     3301 ggtttgcagc gtcggttcca ccaccagctg gttgatacgg ttggtttcaa gaccaccgat
     3361 atctttcagg aagtctggcg ctttgatccc ggctgcccac accatcagat cagcctcaat
     3421 atattcgcca tctttagtat gcaggccgcc ttcatcagca ctggtgacca tggtttgcgt
     3481 cagcacgcga acgccaagtt tcgttaactc attatgggcc gcagcagaga tacgtggcgg
     3541 taatgcaggc aaaatacgtt cgcccgcttc taccagcgtt acgttcaggg cttcgttggt
     3601 cagaccttta tagccgtagc tgtgcagttg cttgaccgcg ttgtgcaatt cagcggagag
     3661 ttctacaccc gtcgcgccgc caccgacaat cgcaatgttc actttgccat tcgcgcccag
     3721 gttggcggag tatttcagga acagattcag catctcctgg tggaagcgac gcgcctggtg
     3781 cgggttatcg aggaaaatgc agttctcttt gacacctggt gtattgaaat cgttagaggt
     3841 gctacccagc gccattacca gagtgtcata ggcgatttta cgttccggaa ccagcagttc
     3901 acctttctcg tcgcgcagtt ctgcaatagt gattgttttc gcttcacgat caatatcaat
     3961 gacggaaccc agctggaact ggaaaccatg attgcgcgca tgggccagat agctcaacgc
     4021 atcgacgcct tcatcaagcg agccagtcgc tacttcgtgc agcagcggtt tccacaggtg
     4081 gctgtggtta cgatcgacca gcgtaatttt ggcttttttc ttgcgtccca gcttatgccc
     4141 cagctgtgtt gccatttcca gcccaccagc accgccgccg acaatcacaa tttttttcaa
     4201 tggcgtagtc aacgtgaccc ccttaaattt attaaccaat tgttaattaa tggttattaa
     4261 catggctctt aattaacaac aggttacagc aatgaaaatg ttcttcgcta atgagaataa
     4321 catgaatggt gcattggtca taccaaaatt gatgttaatc aagttttgtt gctgaaaaga
     4381 taggcgactt taccgggtga gatcaaaaaa cagagtttgt tgtttattat ttgagcgaat
     4441 ttgtgagagt gatatagagt gaggtgtgta tggagggcgt tcggtaggcc tgataagaag
     4501 cgcatcaggc attgatgctc gactgtcgga tgcggcgtga acgccttatc cgacttacag
     4561 aattcggagc gcatcaggta ccggcaaggt tacctgatgc gacgagggca tttacccgag
     4621 ggttttgaac gctttaatgc gctgcaaatg cggggagata ttcttaaatt tgtgcgtctg
     4681 ctcttcgtcc cagacaatct cgtaataatg atgcaactct tcagatgtcc gctggctgtt
     4741 aagcgcttca tcattacgcg acaaaatcac caggcaacga tcgcgattct tttcgcggaa
     4801 gttggtcaca cacttagtgg caatatcggc atactcttcc gggcgatcaa tcttgccttc
     4861 catgttctca taagggaaca aattagggtt gaagatcacc tggcggatgt cgcaaagaaa
     4921 accaatccgt tctgcccagt atccgcccag accaacgccg caaattagcg gacgctcgtc
     4981 aacgttcagt tgcaacattt tgtccacttc tttaagcaga tgctgcatat catgtttcgg
     5041 atgccgcgta ctgtagctta tcaagcgtac atccgggtca ataaactgca attgtaagac
     5101 tttctcgtgg ttacccggac tgttagagtc aaaaccgtgt aaatagataa tcatcgcttc
     5161 ctcacataag ccagggttag tgacctgctt tctcttcctg ccagcgttca tgtaactgat
     5221 tcagacgggt gctgatcgct ttccagcgag ccgagtccat cagttcctgt cgcgaaaatg
     5281 aacctttatg atacaaacgt gtaacacgtt ctgccttgat cggtgacaga ttatctaaca
     5341 cgctgacggc ccctttacga ttattgcaga ccaggatcat atcgcaacct gcgtccagtg
     5401 atgcctgccc gcgttcggca taactgccca taatcgcggc tccttccatc gataaatcgt
     5461 cagagaaaat cacgccatca aaacccagtt cctgacgcaa aacggttttc agccaatagg
     5521 gagaaccgct cgccggacgc ggatcgacat cactgtagat cacatgcgca ggcataattg
     5581 cgtcgagttt attttcgcgg attaaggaac tgaagaccga catatcttta gcgcgaatct
     5641 ccgcttgcgg acgtgggtca cacggtgttt ctttgtgtga gtctgccgtt actgcaccgt
     5701 gtcctgggaa gtgtttcccg gtagttttca ttccggcttc atgcatacca tcaataaacc
     5761 ggctggcaat tgccagggct ttttcgggat cggcatgata agaacgctcg ccaatcgccg
     5821 cgctgatatg cccgacgtcc agcactggcg caaagctgat atcaatatcc atagcaatca
     5881 tttcgctggc catcaaccaa cctgcctcct gcgccagttt gccaccctct tccattccca
     5941 acagcgcagc aaatgattgt gccgctggca agcgggtaaa accttcacga aaacgctgca
     6001 cgcgtccacc ttcctgatca accgccacca ccagatgatt gcgcgaagct gcgcggatct
     6061 ggcgcaccag ttcacgtaac tgggcaggat catgatagtt acgcgtaaag agaatcagcc
     6121 ctcccaccag cggatgcgcc agtatttcac gctcttccgc gtccagttcg taaccttcga
     6181 catccaacat tactggaccc acactgctct ccttattgtt ttattaatag ctgccgccag
     6241 gtgtcatcgg ccagcctgat aaattgttga tcgccggttt gtcgccagcg gtactcaaac
     6301 caccctgctt tgagcatcag cagccaggga aaccatcgcc tgacctgacg ccataattgc
     6361 gccggataaa tcttcgcgcg agtggcatag tcattgacca attgccggtg ctggtcagta
     6421 ttttccaccc acaccgccgc cagttccagc gcgatatcac catctccggc atactcccag
     6481 tcgatgagtt ttaaccctga cgcgctatgc actaaatttc cggcgtggac atccatatgc
     6541 aatggactta agcgtaaagg ccgtggttcc cgcgctttgc gcagacgttt taaccttcgc
     6601 agccaaccca ctgtccgccg agccggatcg ctttgctgcc agtacagttc cagtaacggc
     6661 aacagcgtta ttcgccagcc aaaacgcggt tgttgatgta gataatacag caagcctgcc
     6721 agttcgttgg tatccggcaa atacgttttt acctcgccgg gcagatagtc gaccaccatc
     6781 cagtcacgga gatataaatg cggcttcggt gcaatgcatg cgggtagttg tgataaagcc
     6841 cgatactggc gcaagaacgc gaaccgcggc gcatcaggat cgtgcggctg acgcacaaca
     6901 aaacgctggc cctgatgttc aatgagaaaa ctcccgccac taagcccact attaaacgtc
     6961 gtgacgggat gaaactgcgg gaaaaagcgc gacagcaatt cgtcgcgcgt gatgggatta
     7021 ttgctgcgaa acggcacctt tacctgacca gataatttcg cccgtctgca ccagcatcag
     7081 ctgcatttgt agggtcggag cgttaacgtt gccagaggcg ctggagtaca gcacgtaatg
     7141 agcgccgaca ttgcgggcaa tgcctatggc tttactacgg gtgcctaaac tgtcctgcgg
     7201 cgacaaacct aactgttgct tagccatcga cagctgctgg gcggaaacca gggtaaattt
     7261 accgttatta gccagcgcat ttcgcagcgt ttcggtcgct tctgcggcat tcagcgaacc
     7321 gttagtacgg ttgttaacgc tatcaaccag caggacgcta cccgcagtca cgccgtcagc
     7381 cccaagcatc ttactgacca tcggctgcat tgcgccattc cagtcataat ggcgaatatg
     7441 cggcgcaggc ggtgcagttt gatcttcgtg ctcaattggg cctggctgct gcggtattgt
     7501 cggcaccgat ggcactgtgg ggacaggctg ttgtggctcg gctggttgtt ccggcgctgg
     7561 tttcacttct tctaccggcg caggttcacg ttgccccaca cacccggcga gaaacatcgc
     7621 cagcgcggta atcaaggcgt agcgactcat ttttgtcatc aagattcacc ccttacaaat
     7681 atagataaag tctgactttg tgcgccccca gaaaattggc gctgccgtac agcgttaccg
     7741 ccgaatgtgc gggaatggta acgctgcgtg gcctttccag aggatgcatc tccagccctc
     7801 tggcgtcata ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt tcgttataaa
     7861 gcgttgagga tgctgaaggt tgaatatcag acgtcgaaag gacgggcttt tctgcactga
     7921 tgcccgcagc caataaagat gactccatca ccagcgattg ctcatcattc accggaattt
     7981 ccggatgtga acgacagccc accagcacta acaacgccag agacaccagc ccaaagcatc
     8041 cttttctcat cgttacagac ctttatgcgc cagcattggt cccagcggac ggccacccaa
     8101 caagtgcata tggatgtggt aaacctcttg tccgccgtgg cggttggtgt tcatgatcag
     8161 acgatagcca tcttcggcaa taccttcttg ctcagcaatt tttgccgcta cggtgatcat
     8221 gcgtcccagc gcctgctcat gctcagcaga cacgtcgttc acagtcggaa tgaggatatt
     8281 cggaatgatc agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag
     8341 atcatcctgg tagacgatat cggaggggat ctcacgacga ataattttgc tgaatatagt
     8401 ttcttctgcc acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc
     8461 gccacactgc gagtatagag tatggtcgag ttaaacctgc tctttcaacc ttaactcacg
     8521 atttcttaag caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc
     8581 tgcgaatgcg ctgaaaagac attctctgtt tggtttacac gtcaaatatg aatgcgtata
     8641 tttctcattt gcatttacat gcaaaattat tcgcacacta aatataaccg ctcatcaccc
     8701 tcctccggca tgagcctgac gacaacataa acctagagat ttcaaatgct ttcaacacaa
     8761 tttaacaggg ataatcaata tcaagccatc accaaaccgt cactacttgc cggttgcatt
     8821 gtactggcac tattaccttc tgccgctttt gctgcaccag ccactgaaga aacggtgatt
     8881 gttgagggtt cagccacagc tccagatgat ggcgaaaatg attacagcgt aacgtctact
     8941 tctgcaggaa ccaaaatgca gatgactcaa cgtgatattc ctcagtcggt cactattgtt
     9001 agccagcagc ggatggaaga tcagcagttg caaacgctgg gcgaagtgat ggaaaacacg
     9061 ctggggatca gcaaaagtca ggcggattcc gatcgtgctc tttattattc ccgcggattc
     9121 cagatcgata actatatggt tgatggtatc cccacctatt ttgaatcgcg ctggaatctg
     9181 ggcgacgcac tttctgatat ggcactgttt gaacgcgtag aagtagtgcg tggcgcgaca
     9241 ggactcatga ccgggacggg taatccatct gcggcaatta atatggttcg aaaacacgcg
     9301 accagtcgtg aatttaaagg cgatgtctcg gcggaatacg gtagctggaa caaagaacgg
     9361 tatgtggcgg atttacaaag cccactcacc gaagatggta aattccgcgc gcgaattgtc
     9421 ggtggctacc agaataacga ctcatggctg gaccgctaca acagtgaaaa gaccttcttc
     9481 tccggcattg tcgatgctga tttaggcgat cttacgacgc tttcagccgg ttacgaatat
     9541 cagcgcattg atgttattaa cccaacctgg ggcggtttac cgcgctggaa tactgatggc
     9601 agcagcaaca gttacgatcg cgcacgcagt accgcacctg actgggcgta taacgataaa
     9661 gagatcaaca aggtctttat gaccctcaag cagcggtttg ctgatacctg gcaagcgaca
     9721 ctgaatgcca cccactccga agtcgaattt gacagtaaaa tgatgtatgt cgatgcctat
     9781 gtaaataaag cggatggtat gctggttggg ccatacagta attatggacc tggctttgat
     9841 tatgtcggcg gcaccggttg gaacagcggc aaacgtaaag ttgatgcgct ggatttgttt
     9901 gctgacggta gttatgaatt gtttggtcgt cagcacaatc taatgtttgg tggcagttac
     9961 agcaaacaaa acaatcgtta cttcagttca tgggcaaaca tcttcccgga tgaaattggc
    10021 agtttctaca actttaatgg caatttccca caaaccgact ggtcaccaca gagcctggcg
    10081 caggacgata ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat
    10141 ccgctgcatc tgatcctcgg cgcgcgatat accaactggc gggttgatac gctgacttac
    10201 agcatggaga aaaaccacac cacgccttat gctggtctgg tgtttgacat caatgacaac
    10261 tggtcgacct acgccagcta tacctctatt ttccagccgc aaaatgatcg tgacagttca
    10321 ggcaaatatc tggctccaat caccggtaac aactacgagc tgggtctgaa atcggactgg
    10381 atgaatagcc gtctgatcac cacgttagcc atcttccgta ttgagcagga taatgtcgct
    10441 cagtccaccg gtacacctat ccccggcagc aacggcgaaa ccgcctataa agcggtggat
    10501 gggacagtca gtaaaggtgt ggaatttgaa ctcaacggcg caattaccga caactggcag
    10561 ctgacgtttg gcgcaacacg ctatattgca gaggataacg aaggaaacgc cgttaatcct
    10621 aatctgccac gcaccacggt taaaatgttc accagctacc ggttgcctgt catgccagag
    10681 ttgacggtcg gcggtggtgt taactggcaa aatcgcgtgt ataccgacac cgtgacaccg
    10741 tatggcacct tccgcgccga gcaaggtagc tacgcgctgg tggatctctt cacccgctac
    10801 caggtgacga aaaacttctc gttacagggg aacgtcaata acctgttcga caaaacctac
    10861 gataccaacg tggaaggttc tatcgtctac ggcgcaccgc gtaatttcag cattaccggc
    10921 acgtatcaat tctgaataac acctgtaaaa aaggcagcca tccggctgcc ttagtctccc
    10981 caacgtctta cggattagtg gttacggatg tactcatcca tctcggtttt caggttatcg
    11041 gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc
    11101 agtttcttca ggccagcctg atccacttta gacacatcag caacgctgac gcgcagacgg
    11161 gtaatacatg cgtcgaggtt agtaatgttt tctttaccac caaatgcagc aaccagagcc
    11221 ggtgccattt cgctggtacc tgtcgctttt gcatcttcag tcgcgtcttc acgacccggc
    11281 gttttcagat ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac aatcgcataa
    11341 ccgataccga cgatcgggaa cagccacagt ttgctgctgt taccagacag aacgatgaag
    11401 tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc ccagaagaat acagattggg
    11461 aatgccaggc ctgccagaat cgcgtggatg atgtacagga tcggcgcaac gaacatgaag
    11521 gagaactcga tcggctcggt gataccggtc aggaacgagg tcagcgccgc ggagatcata
    11581 ataccgccca ctttcgcgcg gttttctggt ttagcagagt gccagatagc aattgcggca
    11641 gctggcagac cgtacatttt gaacaggaag ccaccagaca gtttacccgc agtcgggtca
    11701 cccgccatat aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca
    11761 ccaatttgca tctggaaagg tacgttccag atgtggtgca gaccaaacgg taccaggcaa
    11821 cgttcgatga aaccgtaaat gccaaacgca actaccgggt tctggtaagc agcccactga
    11881 gagaaggtct ggattgcaga accaatcggc ggccaaatga aggacagcac aacgccagta
    11941 aagatggcag ccaggccaga aatgatcggc acaaagcgtt taccggcaaa gaagccaaga
    12001 tactcaggca gcttaatacg gtagaaacgg ttaaacatat acgctgcaat cgcaccggag
    12061 ataatccctc cgagtacgcc agtatccgcc aggtgtttag aggcgatttc ttcagcaggt
    12121 aaatgcagta ccagtggcgc aaccacggcc atggttttaa ccatgatgcc ataggcaaca
    12181 actgcggcca gcgcggatac gccatcgtta ttggtaaagc cgagggcgac accgatcgca
    12241 aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt ctgccataac atgcgataca
    12301 acggcgggca gccagctgaa attcgcggaa ccgacgccca gcagaatacc tgcgataggc
    12361 agtacggata ccggcagcat cagcgattta ccgacctttt gcaggttagc aaatgcattc
    12421 ttaaacataa ttgagagtgc tcctgagtat gggtgctttt tttacgttct cacgcgtggc
    12481 aaggggggag agcctcgccg tgtacagggc atctaagcgc cctttattta ttacacagag
    12541 taaaataatt cagtgccaat ttgtttgacg gctatcacgt ttcaacttca gatcattgga
    12601 gaagttgcac attttgacaa aacatatgtc acaatatttc aaacgtggtt attcaccgcc
    12661 caattttcgg cggtgaactt tactcgtttt agccattaat tacgagcttt aaaaaaactc
    12721 attcaacgga tggattgaag gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg
    12781 gtggttacct gcgccagttc ttcaacggca acacctttca acacagccat gtattctgca
    12841 acgtcacgaa ccatcgcagg ttgattctct tttcctcgat gcggaaccgg tgcaaggtaa
    12901 ggtgagtcag tttctaccag taaccgatcc agggggacat aacgcgcagc atcgcgcagt
    12961 tgctccgcat tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt
    13021 aatttacccg ccgtttctct gtcctctgta aaacagtgta gtacgccacc gcaatccgtc
    13081 actttttctt cgcgcagaat tgccagcgta tcggcgcggg cgtcacgggt atggacgata
    13141 accggcttgt tcagttcacg accaatctgg atatgatgaa tgaaggactc ttgctgacgt
    13201 actttagttt ccggtgtgta ataataatcc agaccggttt cacccagcgc tacaacaccc
    13261 tcttctgccg ccagacggcg taaatcttct acatcgtagg gatcattctg gttgagtgga
    13321 tgcacgccac aagaaaatac gacgttgtca cgttcgccta ccagatcccg catatgtaaa
    13381 taacctggta atgttgtggc gactgccaga caaaatttca catcgcgtgc ggcggctttc
    13441 gccagaacgt catccacgtc cttatgcaaa gattcataat ccagaccatc gagatggcag
    13501 tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga ggaaccggta gcacaacgcc
    13561 cggttgcagg taatgctcaa tacgcagtaa aagatcggtg atgagaagct cgcggttgat
    13621 gcctgtgaca gacattaact gttcacgaat gtggcaaaca tcccccagta tagcctgcag
    13681 gcgcgaggga gaaagatggt ttgccagttc gacgaccagt cccggcacat caacattggt
    13741 cacctgcgca gcgccatgat ggcgttttag cgcatccatc agcaacgttg ccagccagtg
    13801 taaacgcgcc ggagcttgtt catgattaag ggccgctagc agcgaatacc agtcgcccga
    13861 tggcacgcta tatgccaacg cctgacacaa tgtttcacga gcctgccagt tatctccctg
    13921 aaacaacgcc agtgccgcgc caggcgaacc ggcgcttaag cgcaatgcgg caagtaatgc
    13981 atcctgtgac attgtcactt cgcgtgaaag ccaggtcacg gcgtactgtt ccggcggcgg
    14041 cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc agtaaacgtt caggctcgcg
    14101 ggtagccagg aaaaaccagg tttctgctgg tggctcttca agcgttttca gcaatgcgtt
    14161 agccgcggcg tcggtcagta aggcagcatc ggttacccag acaactttcg caccacctaa
    14221 gcgggcgtgc tcattcagtt tttcagtgac ctcacgtacc gcatcaatgc ccagcgtatt
    14281 tttccctttt tcgggagcca gggtgtaata atcgggatgc gtgccagcct gcatcaactg
    14341 acatccacga cagtgaccgc aacttttgtg gccctgcggt tgttggcaga gtaaataacg
    14401 gctcagggcg tagattaaag catcatcgcc catgcccggt aacgcctgaa tgagcagcgc
    14461 atggtgacct cttccggcct gatagctggc taccagtttt tcgaaatcag gtcgtaacca
    14521 tggataccat ctcatgcgtc caactccttc acccaattgg tcacggtagt gcggattgca
    14581 tccatcacgg actccagcgg ctgggtggca tcaatggtat gaatgctttt atcttgtgct
    14641 gccagttcca gatagcgggc gcgggtgcga ttaaagaaat cgaaagactc ttgctcaata
    14701 cgatccagct cgccgcgcgc acgcgcgcgt tttaagccaa cttccggggt aacatcgaga
    14761 tagagcgtta agtcggggcg aaaatccccg agaacagcat cacgcagtgt tgccagcata
    14821 tgttggtcaa taccacgtcc gccgccctga tacgcctgag tggagagatc gtggcgatcg
    14881 ccaatcaccc aggtgccgtt agccagcgca ggtttgatga ccgtttctac cagttgaacg
    14941 cgcgcggcat aaaacatcag aacttcggct ttatcggtaa tgacttcatc gcctaccgat
    15001 ttgatatcca gtaccaggct tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga
    15061 gtgaaaacca tgtcgcggat acccagttgc tcgagcgtct caaccaccac gttacgcgcg
    15121 gtagttttgc ctgcgccttc cagcccctca atgacgatat acttactgcg catttttttc
    15181 cttaagcact ttcagataat cctgcacaga cttgttatga ctggcaagat tggtattaaa
    15241 cgtgtgacca cctttaccat cggccacaaa atagagatac ggcgtttttg ccggatgcgc
    15301 agcagccttc agcgaatccg cccccggcgt agctattgca cctggcggca aaccggtaat
    15361 ggtataggtg ttatacgctg tcggcgtttc caggtctgca cgagaaagtt tgccattata
    15421 acgctctccc atcccgtaaa tcacggtcgg gtcggtctgc aggcgcatac caatgcgtaa
    15481 ccggttgata aataccgagg caacctgatc gcgttcactg gcaacggcgg tttctttttc
    15541 gataattgat gccatcgtca ccagctggtt tttatcttta tagggcagac cgtccgcacg
    15601 cccttcccag gcgctatcga ccgctttcac cattttcttg tgcgctcgct tgagtaacgc
    15661 gacatcggtg gtattggcgg tatacatcca ggtgtctggc cagaaccaac cttcaatcca
    15721 ctccgggttt tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt cgctcagcgt
    15781 gtgcttgata tacggggcct cacgcaattg cttgaggtaa tcgctcagac gcatcccttc
    15841 taccagtcgc agagggaact gtgcttcttt accgctttcc agcaatttca gcatctcgcg
    15901 cacggtcatc tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa gatccggttc
    15961 gatacgcagc agccattgga aaacccgtgg gcgattgatg atcttatcgg cataaagctg
    16021 ttcaccgagc gccagacgtc cggtccctgg cttcagggta aatatcgtct cttctttgat
    16081 aagcaatttg ctgtcggcaa gatggcgaac cttccagacg cccacaccag cggcgatacc
    16141 cagtaccacc agcaataaca agataatcaa taacactttt ttcatgacta attcgggcgc
    16201 tcacaaagtg gggctaaata ttcatataac gttgctgacg aaaaggagac atcgccacag
    16261 gcacgtacgg gtatcactgg cattaacgca ttacaaataa ccatctcatc tgcctgcaac
    16321 gcctcctcca gcgaggcttg cacttcgaca agctgataag aggattgtgc cagtaaacgg
    16381 atacagaatt gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg cgtataaact
    16441 acgttgccct tccgccagaa caaattagcc gcacagcatt ccgtaaccca cccttcgctg
    16501 tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat gagagcggat caatacttgc
    16561 tcaagccgat taagatgttt aatacctgca agatgaggat tgcgccccag ccgcaccggg
    16621 cttagcgcca acgtcatccc ctcgttacgc aaacggtcgt aatgtgcggg ataaaccgta
    16681 acggagagaa tccgcgttgc cggtccgctg ttcaatgtgc tgtatcctcg cccgccacta
    16741 ccgcgactga tcaccacttt cagcacacca ttttgctgtt ctgctgccag cgttttcatc
    16801 tctagttcaa gctgaggcca gaagtcacag gaaatcatca accgctgaca agcatcctgt
    16861 agtcgctgga tatgcgccga taacaaactg actttaccgt cgataactct ggcggtggta
    16921 aaacaaccat caccaaactg cgttgcccga tcgcttactg ccagcgattc ctgcttatga
    16981 ccgttaatta agaacatacc ggctccttat cagagaattg ttagtgtggc agcatgttca
    17041 ctacggaaca agtcagaata aaag
//
LOCUS       DAPOZO010000099        16710 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPOZO010000099 DAPOZO010000000
VERSION     DAPOZO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.099"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(46..534)
                     /gene="eco"
                     /locus_tag="ROI21_003728"
     CDS             complement(46..534)
                     /gene="eco"
                     /locus_tag="ROI21_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDX5112601.1"
                     /translation="MKTILPAVLFAAFATTSAWAADSVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            940..1434
                     /gene="napF"
                     /locus_tag="ROI21_003729"
     CDS             940..1434
                     /gene="napF"
                     /locus_tag="ROI21_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDX5112602.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            1424..1687
                     /gene="napD"
                     /locus_tag="ROI21_003730"
     CDS             1424..1687
                     /gene="napD"
                     /locus_tag="ROI21_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDX5112603.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1684..4170
                     /gene="napA"
                     /locus_tag="ROI21_003731"
     CDS             1684..4170
                     /gene="napA"
                     /locus_tag="ROI21_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDX5112604.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            4177..4872
                     /gene="napG"
                     /locus_tag="ROI21_003732"
     CDS             4177..4872
                     /gene="napG"
                     /locus_tag="ROI21_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDX5112605.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4859..5722
                     /gene="napH"
                     /locus_tag="ROI21_003733"
     CDS             4859..5722
                     /gene="napH"
                     /locus_tag="ROI21_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDX5112606.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAAKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5719..6168
                     /gene="napB"
                     /locus_tag="ROI21_003734"
     CDS             5719..6168
                     /gene="napB"
                     /locus_tag="ROI21_003734"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDX5112607.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            6178..6780
                     /gene="napC"
                     /locus_tag="ROI21_003735"
     CDS             6178..6780
                     /gene="napC"
                     /locus_tag="ROI21_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDX5112608.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6799..7416
                     /gene="ccmA"
                     /locus_tag="ROI21_003736"
     CDS             6799..7416
                     /gene="ccmA"
                     /locus_tag="ROI21_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDX5112609.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            7413..8075
                     /gene="ccmB"
                     /locus_tag="ROI21_003737"
     CDS             7413..8075
                     /gene="ccmB"
                     /locus_tag="ROI21_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDX5112610.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGVIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            8117..8854
                     /gene="ccmC"
                     /locus_tag="ROI21_003738"
     CDS             8117..8854
                     /gene="ccmC"
                     /locus_tag="ROI21_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDX5112611.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8851..9060
                     /gene="ccmD"
                     /locus_tag="ROI21_003739"
     CDS             8851..9060
                     /gene="ccmD"
                     /locus_tag="ROI21_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDX5112612.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            9057..9536
                     /gene="ccmE"
                     /locus_tag="ROI21_003740"
     CDS             9057..9536
                     /gene="ccmE"
                     /locus_tag="ROI21_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDX5112613.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            9533..11476
                     /gene="ccmF"
                     /locus_tag="ROI21_003741"
     CDS             9533..11476
                     /gene="ccmF"
                     /locus_tag="ROI21_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDX5112614.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIFAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            11473..12030
                     /gene="dsbE"
                     /locus_tag="ROI21_003742"
     CDS             11473..12030
                     /gene="dsbE"
                     /locus_tag="ROI21_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDX5112615.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            12027..13079
                     /gene="ccmH"
                     /locus_tag="ROI21_003743"
     CDS             12027..13079
                     /gene="ccmH"
                     /locus_tag="ROI21_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDX5112616.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTM
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(13114..13761)
                     /gene="narP"
                     /locus_tag="ROI21_003744"
     CDS             complement(13114..13761)
                     /gene="narP"
                     /locus_tag="ROI21_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDX5112617.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            14081..16672
                     /gene="yejO"
                     /locus_tag="ROI21_003745"
     CDS             14081..16672
                     /gene="yejO"
                     /locus_tag="ROI21_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HDX5112618.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDTTIKGGRLIIEKDGITVFAAIEKGGLLEVKEGG
                     LAFAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKDGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGCITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLLGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFTTLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVNYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWSF"
BASE COUNT         3704 a         4111 c         4822 g         4073 t
ORIGIN      
        1 gtgaccgcct tatccgacct acggggtgcc gcacttcaca gcagtttagc gaactaccgc
       61 gttgtcaatt ttctcttccg ccttccagac gcggtacttc acatccacat tgtctggcgt
      121 atacaccacg atcggcagct tgctgttgta acgcagcatt ccagcatcgc ccagatacgc
      181 ggtgacaaat ttcttctctt ttttgccatc cgggcaggcc atcatcgtcg aaaccgggga
      241 actgacttta tcaaagacat aataatcata gccccagcct tccagcgttt tgctttccag
      301 cttcccgccg agacgatgca aattgcaatc gacttccagc gtctgaccga ttaacagttc
      361 tactttcagg gtagattcat cttcttgcgg ggttaactga atcacctgac gcttcatgcc
      421 tttttcagct tgcggataag gcgcgatttt ttccagtggc tggacgctat ctgccgccca
      481 ggcggaggtg gtggcgaaag cggcaaacaa tactgcaggt agaatggtct tcatttttgc
      541 tttccttctt catttatagg ttttcaccga tgatgtcgct gaaggttaac atattcatca
      601 gatttattaa tttgctttat gaataactta aagaacattc agatgatttt acctgctctt
      661 ttagtgttaa attctgatga gagagcaaac caacagaaat atttctggtg aattaatcgc
      721 tatataaata tgtttataac catttgaaat gtgagcaaaa gcccgttttt gccacgcttc
      781 ccgccactct tttgatcctg ctacaggttt taccccgatc ggggtatgca tctttgacac
      841 atcctttaat atcttagcgg ctataaaaat ggcttattaa ttatgcggtt tatttggtcg
      901 ctctcaattt tcagagcgcg ttaatgatgg aaggtcaatg tgaagattga tgcatcccgt
      961 cggggcatac tcactggtcg ctggcgcaaa gccagtaacg gtatccgtcc gccctggtcc
     1021 ggtgatgaat ctcattttct gacccattgc acccgctgcg acgcctgtat taatgcttgt
     1081 gaaaacaaca ttctgcaacg cggcgcgggc ggctatccga gcgttaactt caaaaataac
     1141 gagtgcagct tctgttacgc ctgcgcgcag gcttgccccg aatcgctatt ttctccgcgc
     1201 cacaccaggg cctgggattt gcagttcacc attggggacg cgtgcctggc gtatcagtca
     1261 gttgaatgcc gccgctgtca ggatagctgt gagccaatgg caattatctt tcgcccgacg
     1321 ctctccggga tctaccagcc gcaacttaat agccaacttt gcaacggatg tggagcatgt
     1381 gcagccagct gtccggtatc agccataact gcggagtatc tccatgcaca ctaactggca
     1441 agtttgcagc ctggtcgtgc aggccaaaag cgaacgaatt tcagacatca gcacccaact
     1501 gaacgccttt cccggctgtg aagttgctgt cagcgacgcg ccgagcggtc agttgattgt
     1561 ggtggtggaa gcagaagaca gcgaaacgct gatccaaacc attgagtcag tacgcaacgt
     1621 agagggcgtg ctggcggtgt cgctggttta tcaccagcag gaagagcaag gtgaggaaac
     1681 accatgaaac tcagtcgtcg tagctttatg aaagctaacg ccgttgcggc cgctgcggcg
     1741 gctgccggtc tcagcgtgcc gggcgttgcc cgcgccgttg ttggtcagca ggaagccatt
     1801 aaatgggata aagcgccgtg ccgtttctgc ggtactggtt gcggcgttct ggtcggaacg
     1861 cagcaggggc gtgtggtggc ctgtcagggc gacccggacg caccggttaa ccgtggtctg
     1921 aactgcatta agggctattt cctgcccaag atcatgtacg gtaaagaccg tttgacgcag
     1981 ccgctgctgc gtatgaaaaa cggtaaatat gacaaagaag gcgaatttac cccaatcacc
     2041 tgggatcagg ccttcgatgt gatggaagag aaattcaaaa ccgccctgaa agaaaaaggg
     2101 ccggaatcga tcggtatgtt cggttctggt cagtggacta tctgggaagg ttatgccgcg
     2161 tccaagctgt tcaaagcggg cttccgttcg aacaacatcg acccgaacgc gcgtcactgt
     2221 atggcgtcgg cggtagttgg ctttatgcgt acctttggta tggatgagcc gatgggctgc
     2281 tatgacgaca tcgagcaggc tgacgcgttt gtgctgtggg gcgcaaacat ggcggagatg
     2341 cacccgatcc tctggtcgcg catcactaac cgtcgtctct ctaaccagaa cgttaccgtg
     2401 gcggtgcttt ctacctacca gcatcgtagc ttcgagctgg cagataacgg catcatcttt
     2461 acgccgcaat ctgacctggt gatcctgaac tatatcgcca actatatcat tcaaaacaat
     2521 gcgataaatc aggacttctt cagcaaacac gttaacctgc gcaaaggggc gacggacatc
     2581 ggctacggtt tacgtccgac ccatccgctg gaaaaagcag cgaagaatcc aggttctgac
     2641 gcctccgaac cgatgagctt tgaagattac aaagccttcg ttgccgagta tacgctggaa
     2701 aaaaccgccg aaatgactgg cgtaccaaaa gatcagctgg aacaactggc gcagctgtat
     2761 gccgatccga acaagaaagt catctcctac tggacgatgg gcttcaacca gcatactcgt
     2821 ggcgtgtggg ccaacaacct ggtctacaac ctgcacctgc tgaccggcaa aatttcccag
     2881 ccgggttgcg gtccgttctc cctgaccggg cagccttccg cgtgtggtac tgcgcgtgaa
     2941 gtgggcacct ttgctcaccg tctgcctgcg gacatggtgg tgactaacga gaaacatcgt
     3001 gatatctgcg agaagaagtg gaatatcccg agcggcacca ttccggcaaa aatcggtctg
     3061 catgcggtag cacaagaccg tgcactgaaa gacggcaagc tgaatgttta ctggaccatg
     3121 tgtaccaaca acatgcaggc cgggccgaac atcaatgaag agcgtatgcc gggctggcgc
     3181 gatccgcgca atttcatcat cgtctccgat ccgtatccga cagtcagtgc gctggcagcg
     3241 gacttgatcc tgccgaccgc aatgtgggta gaaaaagagg gcgcttacgg taacgccgaa
     3301 cgccgtactc agttctggcg tcagcaggta caggcaccgg gcgaagcgaa atcggatctc
     3361 tggcagttag tccagttctc ccgccgcttc aaaactgaag aagtatggcc ggaagagctg
     3421 ctggcgaaga aaccggaact gcgtggcaaa acgctgtacg aagttctgta tgccaccccg
     3481 gaagtgagca aataccctgt atccgaactg gcggaagatc agctgaacga tgaatcccgc
     3541 gagctgggct tctatctgca aaaagggctg ttcgaagagt acgcatggtt tggtcgcggt
     3601 cacggtcacg atctggcacc gttcgatgac taccacaaag cgcgcggtct gcgctggccg
     3661 gtggtgaacg gtaaagaaac acagtggcgt tacagcgaag gtaacgaccc gtacgtgaaa
     3721 gcgggcgaag gctataagtt ctacggtaaa ccggatggca aagcggtgat cttcgcactg
     3781 ccgttcgaac cggcggcgga agcaccggat gaagagtacg acctgtggct ctctaccgga
     3841 cgcgtcctgg agcactggca caccggcagt atgactcgcc gtgtgccgga actgcatcgc
     3901 gccttcccgg aagcggtcct gtttattcac ccgctggatg cgaaagcgcg cgatctgcgc
     3961 cgtggcgaca aagtgaaagt ggtttctcgc cgtggcgaag tgatctcgat tgtcgaaacg
     4021 cgcggtcgta accgtccgcc gcagggcctg gtgtacatgc cgttcttcga cgccgcacag
     4081 ctggttaaca aactgacgct ggacgcgacc gatccgctct cgaaagagac ggacttcaag
     4141 aagtgcgcgg tcaaactgga gaaggtgtaa gccattatgt cccggtcagc gaaacctcaa
     4201 aatggtcgcc gccgctttct gcgcgatgtc gttcgcacag ctggcgggct ggctgccgtg
     4261 ggtgtggcgc tggggttaca acagcaaacc gcacgcgcat ctggcgtgcg gttgcgcccg
     4321 cccggagcca taaacgagaa cgcctttgcc agtgcctgtg tgcgttgcgg tcagtgtgtt
     4381 caggcttgcc cttatgacac cttaaaactg gcgacgctgg cttctggcct gtcggcgggc
     4441 acgccatatt ttgtcgcacg ggatattcct tgcgaaatgt gtgaggacat tccgtgcgcc
     4501 aaagtgtgcc cgagcggtgc gctggatcgt gagatcgaat cgatcgacga cgcgcggatg
     4561 gggctggcgg tactggtgga ccaggaaaac tgtctcaact ttcaggggct gcgctgcgat
     4621 gtttgttatc gcgaatgccc gaaaattgat gaggcaatca ccctggagct ggagcgcaac
     4681 acgcgtaccg gtaagcacgc ccgctttctg ccgacggttc acagcgacgc ctgtaccggc
     4741 tgcggtaagt gcgaaaaagt gtgcgtgctg gaacaaccgg caatcaaggt gttaccgctg
     4801 tcactggcga aaggggagtt aggtcaccat taccgcttcg gctggctgga ggggaacaat
     4861 ggcaaatcgt aaacgtgacg ccgggcgcga ggcgctggag aaaaaaggct ggtggcgcag
     4921 ccaccgttgg ctggtgttac gtcgcctttg tcagttcttc gtgctgggga tgtttttgag
     4981 cggtccgtgg tttggtgtgt ggatcttgca cggcaactac agcagtagcc tgttattcga
     5041 caccgttccg ctgaccgatc cgctgatgac gctgcaaagt cttgccagcg gtcatctgcc
     5101 tgccacggtg gcgttgaccg gtgcggtaat tatcaccgtg ctctacgccc tggcagcaaa
     5161 gcgattattt tgcagctggg tctgcccgct gaacccgatt accgacttag cgaactggtt
     5221 acgcaggcgc tttgacctca atcagtctgc gacgatccct cgccacattc gctacgtgct
     5281 gttggtggtc attctgctgg gatcggcctt aaccggcaca ctcatctggg aatggatcaa
     5341 ccccgtttcc ctgatggggc gcagtctggt aatgggattc ggcagcggcg cgctgcttat
     5401 tctcgcgctg tttttatttg atctactggt cgttgaacac ggctggtgcg ggcacatttg
     5461 tccggttggc gcgctgtatg gcgtgctggg tagcaaaggt gtcattaccg ttgcggccac
     5521 cgatcggcag aaatgtaacc gctgtatgga ttgttttcat gtttgcccgg aaccgcatgt
     5581 gctacgtgcc ccggtgctgg atgaacaaag cccggtgcag gtcaccagcc gcgattgcat
     5641 gacgtgcggt cgctgcgtgg atgtctgttc tgaggatgta tttacaataa ctacacgatg
     5701 gagttcggga gcgaaatcat gaaaagccat gacctgaaga aagcgctgtg tcaatggacg
     5761 gcgatgctgg ccctggtggt gagcggcgcg gtttgggcgg ctaatggcgt cgattttagc
     5821 cagtcgccgg aagtatccgg gacgcaggaa ggggccattc ggatgccaaa agagcaggat
     5881 cggatgccgc tgaactatgt gaatcagccg ccaatgatcc cgcatagcgt tgaaggttat
     5941 caggtaacga ccaataccaa ccgctgcttg cagtgccacg gtgtcgaaag ctatcgcacc
     6001 actggcgcgc cgcgcatcag tcctactcac tttatggaca gcgacggcaa agtgggcgcg
     6061 gaagtggctc cgcgtcgtta tttctgtctg caatgtcacg taccgcaggc cgataccgcg
     6121 ccaatcgtgg ggaatacctt taccccatca aaaggttacg ggaaataaga ggttattatg
     6181 ggaaattctg accgtaagcc tggtctgatt aagcgcctgt ggaaatggtg gcgtaccccc
     6241 agccgtctgg cgctggggac gctgctgttg atcggctttg ttggcggcat cgtcttctgg
     6301 ggcggcttta acaccgggat ggaaaaagcc aataccgaag agttctgcat tagctgccac
     6361 gaaatgcgta acacggtgta tcaggaatac atggattccg tgcactacaa caaccgtagc
     6421 ggcgttcgtg cgacctgtcc ggattgtcac gttccgcacg agtttgtgcc gaagatgata
     6481 cgcaagctca aagcaagtaa agagctgtat ggtaaaattt ttggcgttat tgacacgccg
     6541 cagaaatttg aagctcatcg cctgacgatg gcacagaatg agtggcggcg catgaaggac
     6601 aataactcgc aggagtgccg taactgtcac aacttcgagt atatggatac aaccgcccag
     6661 aaatcggttg ccgcgaagat gcatgaccag gcggtgaaag atgggcaaac ctgtattgat
     6721 tgccataaag ggatagcgca caagctgccc gatatgcgcg aagtcgagcc aggtttttaa
     6781 cagggttatt gcgtgggtat gcttgaagcc agagagttac tttgcgagcg ggatgaacga
     6841 accttattta gtggcttgtc atttacgctg aacgcaggag agtgggtaca aatcaccggt
     6901 agcaacggcg cggggaagac aacgcttctc cgtttgctga cggggttgtc tcgccctgat
     6961 gcgggtgacg ttctctggca agggcagccc ttgcatcagg tacgcgacag ctaccatcaa
     7021 aacctgttat ggataggcca tcagccgggg atcaaaaccc ggctgacggc gttagaaaat
     7081 ctgcactttt atcatcgcga tggcgatacc gcacaatgtc tggaagccct ggcgcaggct
     7141 gggcttgccg gattcgaaga tattccggta aatcaacttt ctgccgggca acaacgccgc
     7201 gtcgctttag cgcgtctgtg gctgacccgt gccacgttat ggatcctcga cgagcctttt
     7261 accgcgattg acgttaacgg cgtcgatcgt ctgacccagc gtatggcgca gcatacggag
     7321 caggggggga ttgtgattct gactacccac cagccgctca acgttgctga aagtaaaatt
     7381 cgccgcattt cactgacgca aacgagggcc gcatgatgtt ctggcgcatt ttccgtcttg
     7441 agctgcgcgt agcgtttcgc cacagcgccg aaatcgccaa cccgctgtgg ttcttcctga
     7501 ttgtcattac cctttttccg ctcagtatcg gtccggagcc gcaactgctg gcgcgtattg
     7561 caccgggcgt tatctgggtt gctgcgctgc tttcatcctt gctggcactg gagcgtctgt
     7621 tccgtgacga tttgcaggac ggcagtcttg aacaattgat gttgttgccg ttacccttgc
     7681 ccgccgttgt gctggcgaag gtgatggcgc actggatggt aaccggtctg ccgttactca
     7741 tcctttcgcc actggtagca atgctactgg gaatggatgt ttatggctgg caagtgatgg
     7801 cgctgacgct gctgctggga acgcctacgc ttggctttct cggtgcaccg ggcgtggcgc
     7861 tgacagtggg acttaagcgc ggtggtgtgc tgctcagcat actggtgtta ccgctgacta
     7921 tcccattact catctttgcc accgccgcga tggacgcggc ttccatgcat ttgcccgttg
     7981 acgggtatct ggcaatttta ggcgcgttgc tggcaggcac cgcgacatta agtccttttg
     8041 cgacggcggc agcgttacga atcagcattc aataacgctg gattactgat ttttgttcga
     8101 gtctggtatc gaaactatgt ggaaaacact gcatcaactg gcgatcccac cacggctgta
     8161 tcaaatctgt ggctggttta taccgtggct ggcaattgcc agtgtggtcg tgcttaccgt
     8221 tggctggatc tggggattcg gctttgctcc ggctgattat cagcagggaa atagctaccg
     8281 cattatctac ctgcatgtgc ctgcggcgat ctggtcgatg ggcatttatg catcaatggc
     8341 ggtggcggct tttattggcc ttgtctggca gatgaaaatg gccaacctgg cggtagcggc
     8401 gatggccccc attggtgccg tgtttacctt tattgccctg gttaccggct ctgcatgggg
     8461 aaaaccgatg tggggcacct ggtgggtatg ggatgcacgt ctgacttctg aactggtgct
     8521 gctgtttctg tatgttggtg taattgccct gtggcacgcc ttcgacgacc gccgtctggc
     8581 gggccgtgcg gcaggtattc tggtgctaat tggcgtggtg aatctgccga ttattcatta
     8641 ctccgtggag tggtggaaca ccctgcatca gggatcaacg cggatgcagc aaagtatcga
     8701 tccggcgatg cgttcgccgc tgcgctggtc gatttttggc ttcctgctgc tgtctgccac
     8761 gctgacgctg atgcggatgc gtaatttgat tttgctgatg gaaaaacgcc gtccgtgggt
     8821 gagtgaactg atactgaaaa gaggccgtaa atgacccctg catttgcttc ctggaatgaa
     8881 tttttcgcaa tgggcggtta cgcctttttt gtctggctgg cggtggtgat gaccgttatt
     8941 ccgctggtgg ttttggtcgt gcactcggtg atgcagcatc gcgcaattct gcgtggcgtg
     9001 gcgcaacagc gggcgcgtga ggcgcgttta cgtgctgcgc aacagcagga ggctgcatga
     9061 atattcgccg taaaaaccgc ttgtggattg cctgtgccgt gttggcgggg ctggcgctga
     9121 ctatcggtct ggtgctgtat gcgctgcgct cgaatatcga tctcttttat acgccggggg
     9181 aaattctcta cggcaagcgt gaaactcagc aaatgccgga agtcggtcag cgtctgcgcg
     9241 ttggcgggat ggtgatgccg gggagtgtgc agcgcgatcc caattcgcta aaagtaactt
     9301 tcaccattta cgacgctgaa ggttcagtag atgtctctta cgaaggcatt ttgccggatc
     9361 tgttccgtga agggcagggc gttgtagtgc agggcgagct ggaaaaaggc aatcatatcc
     9421 tcgcgaaaga agtgctggcg aaacatgatg aaaactatac gccgccagaa gttgagaaag
     9481 cgatggaagc taaccaccgt cgcccggcga gtgtttataa ggacccagca tcatgatgcc
     9541 ggaaattggt aacgggctgc tgtgcctggc gttagggatt gcgctgctgc tgtccgtgta
     9601 tccgctatgg ggcgtagcgc gcggagatgc gcgcatgatg gcgtcttccc gcttgtttgc
     9661 ctggctgctg tttatgtctg tggctggcgc atttctggta ctggtcaatg ccttcgtggt
     9721 caacgacttt accgtcacct atgttgccag caactccaat acccagcttc cggtgtggta
     9781 tcgcgtggcg gctacctggg gcgcacatga aggctcactg ctgctgtggg tgctgctgat
     9841 gagcggctgg acctttgcgg tggcgatttt tagtcagcgt attccgctgg atattgtggc
     9901 ccgcgtactg gcgataatgg ggatggtcag tgtcggcttt ttgctgttca ttctatttac
     9961 ctctaaccca ttctcacgca cgctgccgaa tttcccgatt gaagggcgcg atcttaaccc
    10021 gctgttgcag gatccggggc tgatcttcca tccgcctctg ctctatatgg ggtacgtcgg
    10081 tttctcggtg gcgtttgctt tcgccattgc ttctttgttg agtgggcgtc tggacagcac
    10141 ttatgcgcgt tttactcgtc cgtggacgct ggcagcgtgg attttcctga cgctcggcat
    10201 tgtgcttggt tccgcatggg cctattacga actcggctgg ggtggctggt ggttctggga
    10261 tccggtagaa aacgcctcgt ttatgccgtg gctggtgggg actgcgctga tgcactcact
    10321 ggcggtcact gaacaacgcg ccagcttcaa agcgtggaca ttactgctgg caatcagtgc
    10381 cttctcgttg tgtctgctgg ggactttcct ggtgcgttcc ggcgtactgg tatcggtaca
    10441 cgcgtttgcg tctgatccgg cacgcggtat gtttatcctc gcctttatgg tgctggtgat
    10501 tggcggttcg ctgctgcttt ttgccgcgcg tggacacaaa gttcgctcac gcgtaaacaa
    10561 tgcactgtgg tcgcgggaat ctttgctgtt agcgaacaac gttttgctgg tcgccgcgat
    10621 gctggtggtg ttgctgggga cgttgctgcc gctggtgcac aagcaactgg gactgggcag
    10681 tatttcgatt ggcgaaccgt tcttcaacac catgtttacc tggctgatgg tgccgtttgc
    10741 gctactgctt ggtgtcggtc ctctggtgcg ctgggggcgc gatcgcccac gtaagatccg
    10801 caatttattg attttcgcct ttatctctac gctggtgctg tctctgctgt tgccgtggct
    10861 gttcgaaagc aaagttgtgg cgatgacggt gctcggcctg gcaatggcct gctggattgc
    10921 ggtgctggca attgcggaag ctgcgctgcg tatttcacgc ggtacgaaaa ccaccttcag
    10981 ttattgggga atggtggcgg ctcacctggg gctggcagtg acaattgttg gcattgcttt
    11041 tagtcagaac tatagcgttg aacgtgatgt gcgcatgaag tccggcgata gcgtcgatat
    11101 tcatgaatat cgcttcactt tccgtgatgt caaagaggtg actggtccga actggcgtgg
    11161 cggtgtggcg actatcggcg taacgcgcga tggcaagccg gaaacagtgc tgtatgcgga
    11221 aaaacgttat tacaacactg ccgggtcgat gatgaccgaa gcggcgattg acggtggcat
    11281 cacgcgtgac ctgtacgccg cgctcggtga agagctggaa aacggcgcgt gggctgtgcg
    11341 tctttactac aaaccgtttg ttcgctggat ttgggcgggc gggctgatga tggcgttggg
    11401 cggactgctg tgtctgtttg atcctcgcta tcgtaagcgc gtgagtccgc aaaaaactgc
    11461 gccggaggct gtatgaagcg caaagtattg ttaattccgt tgattatctt cctggcgatt
    11521 gccgcggcgc tactgtggca gctggcgcgt aatgccgaag gggatgatcc gaccaatctg
    11581 gaatcggcgc tcattggcaa gccggtgccg aagtttcgcc tcgaatcact ggacaatccg
    11641 gggcagtttt atcaggcgga tgtgctgact cagggcaaac cagtgctgct taacgtctgg
    11701 gcgacctggt gtccgacctg ccgtgcggaa catcaatatc tgaatcagct ttctgcgcag
    11761 ggcatccgcg tggtcggcat gaactataaa gacgatcgcc agaaggcaat cagctggctg
    11821 aaagagctgg gcaatcctta cgcactaagc ctgtttgatg gcgacggcat gttaggactg
    11881 gatctcggtg tctatggcgc gccagaaacg ttcctcatcg acggcaacgg catcattcgc
    11941 tatcgccatg cgggcgatct caatcctcgc gtctgggaag aagagatcaa gccgctgtgg
    12001 gagaaataca gtaaggaggc cgcacaatga ggtttttatt gggcgtgctg atgctgatga
    12061 tctccggctc agcgctggcg accatcgacg tgttgcagtt taaggatgaa gcgcaggagc
    12121 agcagttccg gcaactcact gaagaactac gctgcccgaa atgccagaac aacagcattg
    12181 ccgattccaa ctcgatgatt gccaccgacc tgcgccagaa agtgtatgaa ctgatgcagg
    12241 aaggtaaaag taaaaaagag attgtcgatt atatggtggc gcgttacggc aacttcgtca
    12301 cttacgatcc gccgttaacg ccgctgacca tgctgctgtg ggtgcttccg gtagtggcta
    12361 ttggcattgg cggttgggtc atatacgccc gctcgcggcg tcgggtacgc gtggtgccgg
    12421 acgcgtttcc tgaacaaagc gtgccggaag gtaagcgtgc cggatatatt gtttatctgc
    12481 cgggtattgt ggtggcgtta attgtggctg gcgtcagcta ctaccagacg ggcaattatc
    12541 agcaggtgaa aatctggcag caggccacgg cacaggctcc ggcgttactg gacagggcgc
    12601 tggatccgaa agccgatccg ctcaacgaag aagagatgtc gcgcctggcg ctggggatgc
    12661 gtactcaact gcaaaaaaat ccgggagata tagaaggctg gattatgttg ggccgcgttg
    12721 gcatggcgct gggtaacgcc agtattgcca ccgatgcata cgctactgca tatcgcctcg
    12781 atccgaagaa cagtgatgcg gcgctcggat acgctgaagc gttgacacgt tcatctgatc
    12841 ccaacgacaa ccgcctcggt ggtgaactgc tacgccagtt ggtgagaacg gaccatagca
    12901 atatccgtgt gttaagcatg tatgcgttta atgcctttga gcagcagcga tttggcgaag
    12961 cggttgccgc gtgggagatg atgttgaaac tcttacctgc caacgatact cgccgtgcag
    13021 tgattgaacg tagtatcgcg caggcgatgc aacatttgtc gccgcaggag agtaaataaa
    13081 aagaggcccg atggtgatgc catcgggcta tttttattgt gccccgcgtt gttgcaggaa
    13141 cagaatggtg gccgccacgc gtgagcggac attgagttta cgcagcagat tgcgaatgtg
    13201 tacttttact gtctgctcgg aaatattcaa caccgaggca atctgtttat ttgacagccc
    13261 ctgtgccagc tcgtgcagaa catccagctc gcgctccgtc agcacgctga agggatcttc
    13321 ttccgcgcca aacatttcac gttcacgtaa gtactgattg acgcgttcgc taaagacttt
    13381 gctgcctttc gctccggcac gaatcgcttc cagcaacact tccgggtcgc tatctttcaa
    13441 cagataaccg tctgcgcctg cgtctatcag cgcaaagaca tcgctggagg catctgatac
    13501 agtcaggata ataatttgcg cggtaacgcc atccctgcgc aaggcattga gggtatccag
    13561 gccactcata cctttcatat tgagatccag caagatcacg tcgatatcca gtctattcgc
    13621 cagatcgatc gcgctcgcgc cgtcgcccgc ttcggcgacc acttcaaagc caggatcaag
    13681 ctccagtaac tgacgaacac cgcgtcgcat aagtggatga tcatccacaa tcatcacctg
    13741 aaaaggcgtt gcttcaggca tagtagtctc ctgaggtttt attagaatca ttatgtttga
    13801 gcgtagcgtt ggtaacgcta ccaggcgcgt gaatgttacc ccattttatg tatggacatg
    13861 tagtgaaaac tacaaccttg tcgttcgctt tggcatcatt ttcattataa agacggaatt
    13921 ttgcatcagc ttctcatcaa gaaaaacaac taaaaaatca atcacaatac ttgtgcattt
    13981 ctttaaacag tttttaaaaa ctgactccgg gtatggagct atgggtattt tctttaccca
    14041 atgcttttaa cagcaattaa tttcatagga tgaaagctca atgcatcaat ctggttctgt
    14101 ttctctttgt cgttccgcaa tatctgttct ggtggctaca gcgttatatt cacccatagc
    14161 attggcatca acagttgagt atggtgagac agttgatggt gttgtcctgg aaaaagatat
    14221 ccagctggtt tatgggaccg ccaataatac gaaaatcaat cctggcggag aacagcatat
    14281 taaagaattt ggtataagta gtaatactga aattaacggc gggtatcagt acattgaaat
    14341 gaatggcacc gcagaatact cagtattaaa tgatggttat caaattgttc aaatgggtgg
    14401 cgcggcaaac cagactaccc tcaataatgg tgtgctacag gtttatggcg cagcgaatga
    14461 tactaccatt aaaggcgggc gcttaattat tgaaaaagat gggattaccg tctttgccgc
    14521 tatcgaaaag ggaggattac tggaggttaa agagggggga ttagcatttg cggtagatca
    14581 gaaagcaggc ggtgcgatta aaacaaccac ccgggccatg gaggtattcg gaacaaaccg
    14641 tctcggtcag ttcgatatca aggatggtat tgctaataat atgctgttgg aaaacggcgg
    14701 aagtttgcga gttgaagaaa atgacttcgc ttataatacc actgtggata gtggcggctt
    14761 actggaggtt atggatggcg ggactgcaac tggcgttgat aaaaaagcag gcggaaaatt
    14821 aattgtctca acgaatgcgc tggaagtgag tggtacaaac agtaaaggcc aatttagtat
    14881 aaaagatggc gtgtcaaaaa attatgagct ggatgatggt tccgggctta ttgttatgga
    14941 ggacacgcag gccattgaca ctatccttga tgagcatgcc actatgcaat cgctgggaaa
    15001 ggatactggt acgagagtgc aggcaaatgc ggtatatgat ctcggtcgat cagatcagaa
    15061 tggatgtata acgtattcct ctaaagccat ctctgaaaat atggttatca acaatggccg
    15121 cgctaatgtc tgggctggca cgatggttaa cgtttcagtc agagggaatg atggcattct
    15181 tgaggtcatg aagccgcaaa taaattatgc acccgctatg ttgttgggta aggtagtggt
    15241 ttctgagggc gcttctttta gaacgcatgg agccgtggat accagcaaag cggatgtttc
    15301 gctcgaaaat agcgcatgga ccatcattgc cgatatcact acgacgaacc aaaacactcg
    15361 cctcaactta gccaaccttg cgatgtctgg cgcaaatgtg attatgatgg ctgagccagt
    15421 gactcgttca tctgtgacgg caagtgcgga aaatttcact acgttgacca ccaataccct
    15481 atcgggaaac ggcaattttt atatgcgtac cgatatggct aatcatcaga gcgatcagct
    15541 caacgtcacc ggtcaggcaa caggtgattt caaaatattc gttacggaca ccggtgccag
    15601 cccggcagca ggtgatagcc ttacactggt aacaacgggc ggcggtgatg ctgcatttac
    15661 gttgggcaat gccggaggcg ttgttgatat cggtacgtat gaatatacct tgctggataa
    15721 tggcaaccat agctggagcc tcgcagagaa tcgcgcgcaa attacccctt caaccactga
    15781 tgtgctgaat atggcggcag cacaaccgct ggtatttgat gcagaactgg acaccgtgcg
    15841 tgagcgtctt ggtagcgtaa aaggcgttaa ttacgatacg gcgatgtgga gttcggcaat
    15901 taacacccgc aacaacgtga ccactgatgc gggagctggc tttgagcaaa cattgacggg
    15961 gctgacgctc ggtatcgata gccgtttctc ccgtgaagaa agcagtacaa ttcgcggctt
    16021 gttctttggt tactctcatt ctgatattgg ttttgatcgc ggcggcaaag gcaatgtcga
    16081 tagctacacc ctgggggctt atgccggttg ggagcatcag aacggtgcct atgttgatgg
    16141 ggtggtgaaa gttgaccgtt ttgccaacac catccatggc aagatgagta atggggcaac
    16201 agcgtttggc gattacaata gtaacggcgc gggtgctcat gttgagagcg ggttccgttg
    16261 ggttgacgga ttgtggagtg ttagacccta tctggccttt accggcttta ccacagatgg
    16321 tcaggactac acgttatcaa acggcatgcg cgcggatgtg ggaaataccc ggatattacg
    16381 cgctgaagcg ggaacggcgg taagctatca catggacctg caaaacggta cgacgctgga
    16441 accctggctg aaagccgccg tgcgtcagga atacgccgat tctaaccagg tgaaagttaa
    16501 tgacgatggc aaatttaata atgatgtggc tggaacccgt ggcgtttatc aggctgggat
    16561 aaggtcatcg tttaccccga cgttaagcgg tcatttgtca gtcagttatg gcaatggcgc
    16621 aggggtagaa tcaccgtgga atactcaggc gggtgtggtc tggtcgttct gataacagaa
    16681 aataaacagg ccgtgatgtg tcgcggtctg
//
LOCUS       DAPOZO010000100        16510 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPOZO010000100 DAPOZO010000000
VERSION     DAPOZO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.100"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(100..1263)
                     /gene="pgk"
                     /locus_tag="ROI21_003746"
     CDS             complement(100..1263)
                     /gene="pgk"
                     /locus_tag="ROI21_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDX5112619.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(1313..2332)
                     /gene="epd"
                     /locus_tag="ROI21_003747"
     CDS             complement(1313..2332)
                     /gene="epd"
                     /locus_tag="ROI21_003747"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDX5112620.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            complement(2617..3330)
                     /locus_tag="ROI21_003748"
     CDS             complement(2617..3330)
                     /locus_tag="ROI21_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDX5112621.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLTENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WLELASFCDFSIFIHAPAQILRERLISRKIAGGLTRQVAEAFYARTDGPNVERVLMNS
                     RQANLIVEMTEEGRYHFTS"
     gene            complement(3327..3836)
                     /gene="fumE"
                     /locus_tag="ROI21_003749"
     CDS             complement(3327..3836)
                     /gene="fumE"
                     /locus_tag="ROI21_003749"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDX5112622.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHTILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFSVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(3858..4823)
                     /gene="yggF"
                     /locus_tag="ROI21_003750"
     CDS             complement(3858..4823)
                     /gene="yggF"
                     /locus_tag="ROI21_003750"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDX5112623.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLTDNLRNVAKALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(4820..6097)
                     /locus_tag="ROI21_003751"
     CDS             complement(4820..6097)
                     /locus_tag="ROI21_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDX5112624.1"
                     /translation="MKTKVAAIYGKRDVRLRVFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(6112..7500)
                     /gene="cmtA"
                     /locus_tag="ROI21_003752"
     CDS             complement(6112..7500)
                     /gene="cmtA"
                     /locus_tag="ROI21_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDX5112625.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIDTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(7555..7971)
                     /gene="cmtB"
                     /locus_tag="ROI21_003753"
     CDS             complement(7555..7971)
                     /gene="cmtB"
                     /locus_tag="ROI21_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDX5112626.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEK"
     gene            complement(8285..10276)
                     /gene="tkt"
                     /locus_tag="ROI21_003754"
     CDS             complement(8285..10276)
                     /gene="tkt"
                     /locus_tag="ROI21_003754"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDX5112627.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            10554..11312
                     /gene="loiP"
                     /locus_tag="ROI21_003755"
                     /gene_synonym="yggG"
     CDS             10554..11312
                     /gene="loiP"
                     /locus_tag="ROI21_003755"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDX5112628.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKALSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGNLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(11518..12438)
                     /gene="speB"
                     /locus_tag="ROI21_003756"
     CDS             complement(11518..12438)
                     /gene="speB"
                     /locus_tag="ROI21_003756"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDX5112629.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(12576..14552)
                     /gene="speA"
                     /locus_tag="ROI21_003757"
     CDS             complement(12576..14552)
                     /gene="speA"
                     /locus_tag="ROI21_003757"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDX5112630.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGIFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(14561..14692)
                     /gene="yqgB"
                     /locus_tag="ROI21_003758"
     CDS             complement(14561..14692)
                     /gene="yqgB"
                     /locus_tag="ROI21_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDX5112631.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            14828..15043
                     /locus_tag="ROI21_003759"
     CDS             14828..15043
                     /locus_tag="ROI21_003759"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112632.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            15347..16501
                     /gene="metK"
                     /locus_tag="ROI21_003760"
     CDS             15347..16501
                     /gene="metK"
                     /locus_tag="ROI21_003760"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDX5112633.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         4097 a         4443 c         4043 g         3927 t
ORIGIN      
        1 cttgtcgcct tcctgcaact cgaattattt agagtatgaa aaattgcgtg ctctaaaagc
       61 gcgctgaaac aagggcaggt ttccctgccc tgtgattttt tacttcttag cgcgctcttc
      121 gagcatcgct actgcaggca gtactttacc ttccacgaat tcgaggaatg cgccgccgcc
      181 agtggagatg taggagattt tgtcagcaat gccgaacagg tcgattgctg ccagagtgtc
      241 gccgccgcca gcgatggaga acgcttcgct gtctgcgata gcgttagcca cgatttcagt
      301 acctttgcgg aagttcggga attcgaacac gccaaccgga ccgttccaca gaatggtttt
      361 cgcattcttc aggatttcag ccagttcctg agcggaagca tcaccgatat ccaggatctg
      421 ctcgtcagct ttcacatcgt taacagattt cagggtagcc ggtgcagttt cagagaactc
      481 ggttgctacg cgaacatcag acggaaccgg gatgttgcag gtggtcagca gacgtttagc
      541 ttcgtcaacc aggtcagctt cgtacaggga tttacccaca tcgtggcctt gtgccgcgat
      601 aaaggtgtta gcgataccac caccaacaat cagctggtca gcgattttag acagggagtc
      661 cagaacggtc agtttggtag atactttaga accaccaacg atagccacca tcgggcgagc
      721 aggttctttc agtgctttac ccagcgcgtc cagttcagct gccagcagcg ggcctgcgca
      781 cgcaacgtca gcgaatttac cgataccgtg agtagaagcc tgcgcgcggt gagcagtacc
      841 gaatgcgtcc attacgaaca cgtcacacag tgcagcgtat tttttggaca gggtttcgtc
      901 gtctttcttc tcgcctttgt tgaagcgaac gttttccaga acaaccagtt caccttcagc
      961 aacgtcaacg ccgtcgaggt aatctttaac cagacgaacc gggttagaca gtttgtcttt
     1021 caggtagtta acaaccggca gcagagagaa ttcttcgttg tactcgcctt cggtaggacg
     1081 acccaggtgg gaagttacca tcacttttgc gccttgtttc agggccagtt cgatggtcgg
     1141 cagagaagca cggatacgcg cgtcgctggt tactttcccg tcttttactg gtacgttcag
     1201 atccgcacgg ataaatacac gtttcccagc aagatccaga tcggtcatct taattacaga
     1261 catggtgaat cctctcgttg attctaaaag ttttgcagac gctgcttgcg tcttacctga
     1321 aagcaacagt agccatagct aacgtcgtgt cgagcattcg gttagcaaag ccccattcgt
     1381 tatcgcacca gaccaacgtt ttgatcaggt gtgcgccact gacccgggtt tgggtgccat
     1441 cgacaatggc actgtgcgga tcgtggttaa aatctacaga gaccaacggc aattccgtat
     1501 agtcaactat accatgaaat gcaccttgtg ctgctttttg cagcaacagg ttgacttcat
     1561 tggcttttac aggtttcttc accgtcacgc ttaaatcgat tgccgtcaca tttatggttg
     1621 gcacacgtac cgcaatcgct tcaaagcgat cgttaaattg cggaaaaaat cgtgtgatac
     1681 cggcggccag tttagtatcg accggaatga tcgactggct ggctgcccgg gtgcgacgca
     1741 ggtcaggatg gtatgcatca ataacctgtt gatcgtgcat ggcggagtga attgtggtca
     1801 cagtgccgga ctcaataccg tacgcatcat ctaacaattt gatgacggga attatgcaat
     1861 tcgtggtaca ggaagcgtta gaaacgatgc ggtgttccgc acgaagttga tcctgattga
     1921 cgccgtaaac aacggtcgcg tcgagatcgt tactgccagg atgtgaaaag agcacttttt
     1981 tggccccggc ggcaatatgc gcttcgccat gctcgcggga gccatatacg ccggtgcagt
     2041 cgaggactac atcaacgcca agttcacgcc aggggagcga ttgcagtgaa cgttcatgca
     2101 atacgcggat ggcgtcatca ccaacaaaaa gttgatcgcg ttcctgtcgt acttcccatg
     2161 caaaacggcc atggctggtg tcatatttca acaaatgcgc catgcccgca gcatccgcca
     2221 gttcgttgat tgccaccacg gtaatttccg cccggcgtcc ggattcatac aaagcacgaa
     2281 ccacattacg cccgatgcga ccgaagccat ttatcgctac gcgtacggtc atagatctcc
     2341 tgcaaggttt tccctgagca aatttgccag acagagtaat ccagcaaatc gtccggggaa
     2401 accttacctg tcgcaaactg cgactgattg gttaattgtc gaacatttaa tcgactgaaa
     2461 cgcttcagct aggataagcg aaacgtggaa taaaaggaat gtttgtccag ccgaagaaga
     2521 catttatctg actcacatca cacttttatc cccttttgtg ggaagcttta ttccaggctg
     2581 gcgtaataat aaccctacaa taactggaat aaattgtcag cttgtgaagt gataccggcc
     2641 ttcttcagtc atctctacta tcagattggc ctgacggcta ttcatcagca cacgctcgac
     2701 attggggcca tcggtgcggg cataaaatgc ttcggctact tgccgcgtta atcctcccgc
     2761 aattttacga ctaatcaagc gctcccgcag aatttgagca ggtgcatgaa taaaaataga
     2821 aaaatcgcag aaagatgcca actccagcca cttttcatca tccagtaaca accagtttcc
     2881 ttcgacgatg acgagtggtg cggtaacgtg caacgcatct tcaacaggat catgcttttg
     2941 tcgatcgtac tgcggccacg tacaatcccc ttccacgacc tggcgcagat tttccgtcag
     3001 tttcgcaacg tcaaatgtct ctggtgcgcc tttgaagggg cgcaattgat gcgcatccag
     3061 ccagctattg taatggtgaa aaccatccat cgggagcgtc tgaatagcgg gcagttccgg
     3121 gtcttgttgc gcgagatatt cccagaaggt ggttagggtt gatttacccg tccccggcgg
     3181 agcgcaaaga aaaaccaccg tacgccgctg cggatttacc gtctgtaagg ccgccaacat
     3241 gtgcagcaac ggtttatgta cattttcaat ctcttcgttc tggtactgtg cctgtatctt
     3301 cagcccattc accgttaatt caattttcac ggcgttaatt cctttaaggt ggtcgttact
     3361 gccagcgtca gcgccgtttt gaccattccg ctaaaacgcg cctctgacca gacggagaaa
     3421 tcagcgaact tcatcgcttt taacgcgtca tagagcatcg cattgcgggt tatgctattg
     3481 acgttgctga taaaatccca ggtcatgtca tcggcaaatc cgggcgtttc atcctgttcg
     3541 ttcaggtaat gccagaactg ggaaaaatgc gtgatatcgg cgtacaacca tttatccatt
     3601 ttccccaacg cataaatcaa acgcagtgca acatcaatat cgtcaagcgg gccgctttgg
     3661 gcgagtaacg gcttcactgc atattccacg atctcttcat cgttatcgac aaacagcgac
     3721 ggcagaaact ggcgtatccc ctggagcaaa atggtatgcg cagttttcat aaatgaaaat
     3781 aaattgtcct gtgcatccag ttgctcaagc acatcatctt ctgtcagcgt cgccattggt
     3841 cttccgatag atcaccatca atgcagggag tctattatat tacacgtttg gtccgcgccg
     3901 ccgatcagta atgtctgcgt ccgcacccca ttcgccgtct gttggatacc gttcaccagc
     3961 tcaccgcccg tcacgcccgt agcgctaaag aggatatcgt tccccctcac cagttcatcg
     4021 agcgagtaca cacggttaac gtcaacgccc attgccttgc aacgcttgcg ctcctgctct
     4081 gcaatctgcc gattttccgt gtaatcccct ttagcctggc aaaagtcaat cagctcggcc
     4141 tgcatatccc cgcctaatgc tttaacggca caggcagaaa tcacgccttc tggcgcaccg
     4201 ccgatggtgt acatcacatc gtaaggatta tcctgccagc aggtcagcac gctggcggcg
     4261 acatcgccat ccggcagggc aaaaaccttc acgcccagct gtgttgcttc ctcaatcgcg
     4321 gcactcaggc gcggtttatc cagcgtaacc atgcgcagct tatccagcgg tttgcctaac
     4381 gctttcgcca cgttgcgcag gttgtccgtc agaggcaaag acagatcaat cgcgcccgcc
     4441 gccagccgat taacaaccag ctttttcata tacatatcgg gcgcatgtaa caggctatct
     4501 cgaggggcga aagccatgac cgccagcgca ttgctctgcc ccatcgccac catccgcgtg
     4561 ccttcaatgg gatcaaccgc gatatcgact tctggcccat cgcctttgcc cacctcttcg
     4621 ccaatccaca gcatcggcgc atggtcaatt tctccttcgc cgataaccac tcgtccacga
     4681 aaagcgacgt cgtttaatgc ctggcgcatc gcggtgaccg ccaggccatc aattttattt
     4741 ttgtcgccac atccggtttg cggccaggca gccaacgctg cctgttccgt aacgcgaaat
     4801 aatggccacg ccagggacat cattgcgcgg cctccccgat atcaacgcca aattgtgcca
     4861 gcagatattt ctcggcctgt tcattccaga tgccgtgcgt ttcctcaacc agccgcgcca
     4921 actctttaaa cagcggatcg gttttgcctt tttcggcaaa atcggcaatg gcagtgagcg
     4981 gcatggtcac gccgttataa atgagttttt taccgccagg gatatccggc agattgagca
     5041 cggtttctgg caccgcatcc aggccaccga tatgggtcac cataaacgac ggctgtaact
     5101 gcccagtggc gctaagggca atcgcctctt tcatgtcgtc cgttgaaccg ccagatgtac
     5161 cgacgacgtg cgtgctgttg taatggacgt tgtagaaatt aaacggcact ttgaagtttt
     5221 tatccgtcgg cccggcaaag aagttcagac agccatcttc cgccagtaat tcatcagcca
     5281 tctcaacgac agcaggcacc gccgcataaa caaaaatgtc atcgaacccg gcatctcctg
     5341 tcagcgcccg cagcatctgg acaggatcgc tcatcccttt ggtattcaca tacaccagct
     5401 caatgccttt actggccgcc agttccaccg gcagcagctt ctgtacctgc gccagacgtt
     5461 tgtcgtcgat atcgaccacc accacccgcg acggttgtat gccgccgtta atggcgtaat
     5521 cgatagcgcc aatgcccatc ggacctgcac acgccagcag tgcaatattg ccgccaggtt
     5581 tgacgcccat gcgatgctca taaacatatt gcgtggtgtg ataattggca tgataagcac
     5641 caataatgca gcacataggc tctgccagcg acgccgcagc aaaataagag ccgtgatacg
     5701 gtaatacgca gcccaaatta atggcgattt cgggaataat catataagtg gcattgccgc
     5761 caaaatattc gtagctgtag cccgctgaat atccgcttgg taaccccatc gccggttgca
     5821 atacaaaacg ctggcctttt ttatatttgc cagtgagatt tttacccact tcgacaataa
     5881 ccccggcaca ttcatgcccg gtaatgaccg gatgattttc taaatcgtcg ggtacgcgtt
     5941 tatgttcact accgagtaac gccgctttcc aggtcgataa acagacgctg tcagaaatta
     6001 cactcaccag taattcatta tcggtaattt ctggcagttc aaatacgcgc agacggacat
     6061 cccgcttgcc ataaatagca gcaactttgg ttttcatttt tacctctgta atcagtgttt
     6121 atgttcggcg gttaattgat taaataaagt gtcgagtttt ggatcgccaa tatagttatt
     6181 aatcaatatc agtggtttat ccgtcacacg tttcacgcgc ccttccagac tggcatgagt
     6241 aacgacgata tccgcatccg caggcacgtt ttctatggcg taatgtttta cttcaattgc
     6301 cagccccgct ttttccaggc gtttacggaa ggtggtcgcg cccatcgcac tggagcccat
     6361 ccccgcatcg caaacaaagg cgatacgctt aaccctgctt aacgagaatg caccctcttg
     6421 tttcatcgcc ttaaccgcat tggctgactg agcaaactca tcttcgctct ccgtttccac
     6481 cgttttttcc atcttcagta tcagcgaagt aatagcaaag gacaccaggg tacctaccgt
     6541 aacaccggca attgtcgcca ggaacgagcc tttcggcgtc agtgccaggt aagcaaagat
     6601 agaccccgga cttgggccag ccaccagacc accgtccagt aagttaaaca tccaggtgcc
     6661 agacataccg cccgcaatca tggcaataat ggtcagcggc ttcatcagca catacgggaa
     6721 atacagttcg tggatcccac cgaggaagtg aataatcatc gccccgggcg cagaacgttt
     6781 actcatccct ttaccaaaca aggtaaacgc cagcagcagc cccaggcccg gacctgggtt
     6841 agaggccacc ataaagaaga tggatttacc gttaaccgaa gcctgttgca ttcccagcgg
     6901 ataatagacg ccctgatcga tcgcattatt gaggaaaagc actttcgccg gttcattgat
     6961 gactgacaac aatggcagat aacccgcatg taccagcgcc tcaatacact ctttgacgaa
     7021 agtattggca attaacaccg ccgggccgat aacttcaaaa cccagcagac aaaggagcat
     7081 ccccgcgata cctaatgaga agttattgat aaccatctca aaaccggcag gtatgcgttt
     7141 ttccagtgcc ttatcgacat attttatgac caacccaccg agcggcccca tgatcattga
     7201 gccaaggaac atcgggatct ctgcgccaac gatcacacca atagtatcta ttccgcccat
     7261 gaccgccccg cgtttaccgc cgaccagatg accacctgta gaaccaatca tcacgggcaa
     7321 taaataggta atcatcgggc cgacaatttt ggcgaaatgt tcgttaggca gccaaccggt
     7381 gggaataaat aacgcagtaa taaaacccca ggcaataaaa gcaccaatat tggggatgac
     7441 cattgcagtc aaaaagcccc caaaagcctg gacctttgca cgagcagact tgttttccat
     7501 aatattatcc tgtagaggag agagtaatta tccgcggctg ataatgtccg ctaattattt
     7561 ttctgatgat gctgttaaga gttgttcgag tatttcttct tcgcacagta attcacttaa
     7621 cgcctgaata gcgccaatgt gcgaatcggc atcggcagca gagagtccga tgagtaattt
     7681 aattggttcg tcattccccg gaaaataaac accttgttca agtaatgtca aagacatccc
     7741 ggttttaagc gccccacatt ccggtcgcgc atgaggcatt gccacgcctg gtgcgagaat
     7801 ataataaggg ccattgttaa tggtggaatc tttaattgcc tgaatgtaat tctcgctgat
     7861 atagttttta tccagcaatg ataccatcga gaaatcgata gcttcctgcc aatcttttgc
     7921 tgaatgtata actgagattg atgattctgg aaaataatca ctaagccgca taaattatcc
     7981 ttattttatt tttagggtac cgtccggcgg taaaaataaa atactcgtgc ggtttaatga
     8041 aagcaattga gcagggaaac cggtgttttg aaaacagtga attattaatt tcttatataa
     8101 cattgagtta tagatataac aacaaatgtc aatacgcata tcgtgatgca tattacataa
     8161 ttgatattta ttgcatataa atatttgtga tctacaacac gccttatcta ttgcttgtcc
     8221 gcaaacggac atatcaaggt aataaaaaag gtcgccgaag cgaccttttt acccgaaatg
     8281 ctaattacag cagttctttt gctttcgcaa caacgttatc aacagtgaag ccgaactctt
     8341 caaacagcag ctctgccgga gcagattcac cgaaggtggt cataccgacg atagcaccgt
     8401 tcaggccaac atacttgtac cagtagtcag caatacccgc ttctacagca acgcgtgcag
     8461 taaccgcttt cggcagtacg gattcacggt aagcagcatc ctgcttgtca aatgcgtcgg
     8521 tagacggcat ggacaccacg cgcgctttca cgccttcggc agtcagtttt tcgtaggcag
     8581 caacagccag ttcaacttct gaaccggtag cgatgaaaat cagttccggc tgaccggcgc
     8641 agtctttcag cacataacca ccgcgcgcga tgtttgccag ttgctcttca gttcgttcct
     8701 gctgcgccag gttctgacgg gagaggatca gtgcggtcgg gccgtcctga cgctcaacac
     8761 cgtatttcca cgcgaccgcg gattcaacct ggtcacacgg acgccatgta gacatgttcg
     8821 gggttacgcg cagagaagcg acctgctcaa ccggctggtg agtcgggccg tcttcgccca
     8881 gaccgatgga gtcgtgggtg taaaccatca cctgacgctg tttcatcagc gcagccatac
     8941 gtacggcgtt acgtgcgtat tccacgaaca tcaggaaggt ggaggtgtac ggcaggaagc
     9001 caccgtgcag ggagataccg ttagcaatcg cggtcatacc gaactcgcga acaccgtagt
     9061 ggatgtagtt acccgcagca tcttcgttga ttgctttaga accagaccac agggtcaggt
     9121 tagacggcgc caggtcagca gaaccgccga ggaattccgg caacagcgga ccgaacgctt
     9181 cgatagcatt ctgagacgct ttacggctgg cgattttcgc cggattagcc tgcagtttag
     9241 cgatgaactc tttcgcttta gcgtcgaagt cagacggcat ttcgcctttc atacggcggg
     9301 taaattcagc ggcttcctgc ggataagctt tcgcgtaagc agcgaatttc tcgttccatg
     9361 cggattcttt cgcctggcct gcttctttcg catcccactg agcatagatt tcagacggga
     9421 tttcgaacgg cgcatatttc cagcccagtt gttcgcgggt cagggcaatt tcagcgtcgc
     9481 ccagcggcgc accgtgggag tcgtgggtac cggctttgtt cggggaaccg aaaccgatga
     9541 tggttttgca catcagcagg gaaggtttgt cagtcactgc gcgcgcttct tctactgcgc
     9601 gtttgataga tgccgcgtca tgaccgtcga tgtcgcgaat aacgtgccag ccgtaagctt
     9661 cgaaacgcat tgcggtgtcg tcggtgaacc agccttcaac gtgaccatcg atagaaatac
     9721 cgttgtcatc gtagaatgca atcagtttac ccagcttcag cgtacccgcc agagagcaaa
     9781 cttcgtggga gatgccttcc atcatgcagc cgtcgcccat gaaggcgtag gtgtagtggt
     9841 cgacaatgtc gtggcccgga cggttaaact gcgccgccag cgttttttct gcaatcgcca
     9901 taccgactgc gttggcaata ccctgaccca gcggaccggt ggtggtttcc acaccagcgg
     9961 tgtaacccac ttccgggtga cccggagttt tagagtgcag ctgacggaag tttttcagtt
    10021 cttccatcgg cagatcgtaa ccggtgaggt gcagcaggct gtagatcagc atggagccgt
    10081 ggccgttgga cagcacgaag cggtcacggt cagcccagga cggattctgc gggttgtgtt
    10141 tcaggaaatc acgccacagg acttcggcaa tgtcagccat acccataggg gcacccgggt
    10201 gaccggattt ggctttctgt actgcgtcca tgctcagcgc acgaatagca ttggcaagct
    10261 ctttacgtga ggacattttg actccagatc ggatgatgaa gggcacgccc ttaacgactt
    10321 gacgacagcg cgttttgggc tacgccggaa aatttgccaa caatttaccg caagccgcgc
    10381 gtcatgtaca tggaacatcc ttttgccgct tcagaaatct ctggatcatg ctcgcatgtt
    10441 gcgcaatcta ctcgcccgtc cgctgcgctt ttccttatac tgagactgag cgtcgattca
    10501 cctgcaaacg gcgcattttt agaataatcc tgaccttgtg cggaagagaa aacatgaaaa
    10561 ttcgcgcctt attggtagca atgagcgtgg caacggtact gaccggttgc cagaatatgg
    10621 actccaacgg actgctctca tcaggagcgg aagcttttca ggcttacagt ttgagtgatg
    10681 cgcaggtgaa agccctgagc gatcaggcat gtcaggagat ggacagcaag gcgacgattg
    10741 cgccagccaa tagcgaatac gctaaacgtc tgacaactat tgccaatgcg ctaggcaaca
    10801 atatcaacgg tcagccggta aattacaaag tgtatatggc gaaggatgtg aacgcctttg
    10861 caatggctaa cggctgtatc cgtgtctata gcgggctgat ggatatgatg acggataacg
    10921 aagtcgaagc ggtgatcggt cacgaaatgg ggcacgtggc gttaggccat gtgaaaaaag
    10981 gaatgcaggt ggcacttggt acaaatgccg tgcgagtagc tgcggcctct gcgggcggga
    11041 ttgtcggaag tttatctcaa tcacaacttg gtaatctggg cgagaaatta gtcaattcgc
    11101 aattctccca gcgccaggaa gcagaagccg atgattattc ttacgatctt ctgcgccaac
    11161 gcggcatcag cccggcaggt cttgccacca gctttgaaaa actggcaaaa ctggaagaag
    11221 gtcgccaaag ctcaatgttt gacgaccatc ctgcatccgc cgaacgcgcc cagcatattc
    11281 gcgatcgcat gagcgcggat gggattaagt aaagcctggt ggtgtcgaac gtaggtcaga
    11341 taaggcgttc acgccgcatc tgacatgaat ggcacatttg ttaccttgtg cgcattgccg
    11401 gatgcgatgc tggcgcatct tatccggcct acgggtgccg aacgtaggtc ggataaggcg
    11461 ttcacgccgc atccgacatt aatggcacgt tttacccgtg cgcatcgcat ctggtgctta
    11521 ctcgcccttt ttcgccgcct gaatatacag catttccagc gccagcgttg ccgctgccag
    11581 agcagtgatt tccgactgat cgtatgccgg agccacttcc actacgtcca tcccaacaat
    11641 gttgagatct ttcaggccgc gtaccagttt aatagcgcga tcggaggtca ggccgccaat
    11701 cactggcgta ccggtgcctg gtgcaaaagc aggatccagg cagtcgatat caaaagtcag
    11761 gtaaaccggc atatcaccca caatctgttt cacttgggcg ataacgtcat ccacgctgcg
    11821 atcgttcacc tggcaggcgt ccagcacggt aaagccgttg tctttatcaa actcggtacg
    11881 aataccaatc tgcacggaat gattcgggtc gatcagacct tctttcggcg cggtatagaa
    11941 catagtgccg tggtcaaatt cacaaccgtt cgcataggta tcggtgtggg cgtcaaagtg
    12001 taccagcgcc attttgccga aatgcttcgc atgagcacgc agcagcggca gcgtaacaaa
    12061 gtggtcacca ccgaaagaga gcatacgctt accggcagcc agcagcttct cggcgtgcgc
    12121 ctgcagcttt tcgctcatct cacgggcatc gccaaaggca tataccagat cgccgcagtc
    12181 cacgacgttc agacgctcac gcatgtcgaa attccacggg aagcggttgt gttcccaggc
    12241 cagattcgtc gaaacctgac ggatcgctgc cggaccgtgg cgaccacccg cacgaccaga
    12301 agtggccata tcgaacggca cgccagtaat cacccagtct gcatcgctgt catacggctg
    12361 gaagttcatc ggcaggcgta aaaaaccaaa ggcattggaa accagtgagt tatcgtattg
    12421 atgacctaag gtgctcatgg atggactcct attacaaagt cgatataaaa aaacccttcc
    12481 gcgtcgttag gcccgacgag gaagggttgg atttgtcaca ataaattgtg gcggattatc
    12541 accgctaaat taagcggatt caagtaacac aggacttact catcttcaag ataagtataa
    12601 ccgtacaaac ctgcctcgaa ctcttcaagg aactgttgtt gcagttcagc atcaagatcg
    12661 gttttcttca cttgatcgcg gaactgggtt aacagcgttt tcggatcgag ctgtacatat
    12721 tgcagcatgt ccgccacggt atcgccttcg tcagacagtt ctacttctac gctaccgtca
    12781 gggaagacga acacgtcaac cgcttcggta tcaccgaaca ggttgtgcat gttgccgagg
    12841 atctcctgat atgcgccgac cataaagaaa ccgagcatcg gcggattctc tggatcgtac
    12901 tccggcattg gcattgtcgt ggcaataccg tcaccatcaa tatagtggtc gatagcaccg
    12961 tcagagtcac aggtaatatc cagcagcaca gcgcgacgtt ccggcacttg atccagccct
    13021 tccagcggca gaaccgggaa caactggtcg atcccccatg cgtccggcat cgactggaac
    13081 agcgagaagt tgacgtacat tttgtccgcc atacgttcct gcagctcgtc gataatcgga
    13141 cgatgagcac ggttttgcgg atccagctgc ttttgcactt catggcacat gctcaaataa
    13201 agctgctcag cccatgcacg ttcttgcagg ctaaagatgc cggaagagta gccgatatga
    13261 atgtcgtgca gatccatctg actgtcgtgt aaccattcac gcagagaacg gcgagttccc
    13321 ggttcgtgca tctcctgcca ggtttcccac atgctttgca gcgcgcgcgg cgcatcttct
    13381 gcaggcgcgg tcggcaccgt gtattcgtta cgttccacgc cgatgatatt agacaccagc
    13441 acggtgtgat gcgcagtcac cgcacgaccc gattcggtga ttaccgtcgg atgcggcaga
    13501 ccgttttctt cacacgcatc gccaatcgcc cagataatgt tgttggcgta ttcattgagg
    13561 ccgtagttca ccgaacagtc ggactgcgaa cgagtacctt cataatccac gcccagaccg
    13621 ccgccgacgt cgaagcactg aatattgacg cccagcttgt gcagttccac atagaaacgc
    13681 gcggattcac gaacgcctgt cgcgatatcg cgaatattcg ccatctgcga accgaggtgg
    13741 aagtgcagta gttgcaggct gtcgagacgc ccggcttcac gcagggtttc aaccagttgc
    13801 agtacctgag tcgcagccag gccgaacttc gatttttccc cgccggagga ctgccattta
    13861 cccgaaccct gcgaagccag acgtgcacgc acgcccagac gaggaacgac attcagacgt
    13921 tctgcttcat ccagcacaat ggcgatttct gacatcttct caatgaccag atagaccttg
    13981 tgccccatct tctcgccaat taatgccagg cggatatatt cgcggtcttt ataaccgttg
    14041 cagacgatga cgctacgggt catgccagca tgtgccagta ctgccatcaa ctcggctttg
    14101 gaaccggctt ccagacccag cggttcgccc gaatgaatca gggactcaat cacgcggcgg
    14161 tgctggttaa ctttgatcgg ataaacaagg aagtaatcgc cgttatagcc gtaggattcc
    14221 ctcgcacgtt tgaacgcggc gttaatggaa cgcaaacggt gctgcaggat ctgtgggaaa
    14281 cagaacagtg caggcagacg ctggccctgt gcttcacgag ttttcactaa ctgcgcgaga
    14341 tcgacgcgag cttccgggac gtccgggtcc gggcacacgc taatgtggcc cagctcgtta
    14401 acgtcatagt agttattgcc ccaccaggca atattgtaag tacgcagcat cttgctggct
    14461 tcctgggagc tcattgcaac ctcctgcatg gagcgtagta caccgtgttc gcccgctgac
    14521 gaaggcaaac ccatagacat gtcgtcagac atagcgaacc tcaaattatt ttattaagtg
    14581 taaaacagtt aacgactatc gcagcctgga actgcgataa caacccataa gcacatggat
    14641 tttccagcag tgaatgctga cgctccaact gcgcaaccgg tttctttttc ataacattat
    14701 taagcacata accgaacgta agtgtgaaag ttcggcgaaa ccacgagaaa actcttgttt
    14761 ttacaagagc gcccttgttc agtcctcagt aactgtaacc agctcttgaa tcctgagaag
    14821 cgccgagatg ggtataacat cggcaggtat gcaaagcaga gatgcagagt gcggggaacg
    14881 aatcttcacc agaacggtga gacaggttaa gcagcagaca acggttcatt atttcgtatc
    14941 acctccacgg ccgcctgtta agacgaaccc acaagccaaa actctgattt caacccggct
    15001 ggaagtggca acacgaaaga aacgtcgtgt gctttttatt taagccgcgc gccgcgtttt
    15061 ataccccaca atggcagaaa attgcaaaag ataaatacgc agaatgccgg cattgtcagg
    15121 aaaaatttcc agccacgttt ttaacagaat gagacacgat tcaaaaaaaa gtggaaatag
    15181 ggtgaagaat tgacctaaaa tagccatcca gatgttaatc catccatacc gattaacact
    15241 cagactgcca gtgtttttaa cctgcagagt cgtggtagga tccgctacca cagaaaatcc
    15301 acacaacagt ttgagctaac caaattctct ttaggtgata ttaaatatgg caaaacacct
    15361 ttttacgtcc gagtccgtct ctgaagggca tcctgacaaa attgctgacc aaatttctga
    15421 tgccgtttta gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt gcgaaaccta
    15481 cgtaaaaacc ggcatggttt tagttggcgg cgaaatcacc accagcgcct gggtagacat
    15541 cgaagagatc acccgtaaca ccgttcgcga aattggctat gtgcattccg acatgggctt
    15601 tgacgctaac tcctgtgcgg ttctgagcgc tatcggcaaa cagtctcctg acatcaacca
    15661 gggcgttgac cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc tgatgtttgg
    15721 ctacgcaact aatgaaaccg acgtgctgat gccagcacct atcacctatg cacaccgtct
    15781 ggtacagcgt caggctgaag tgcgtaaaaa cggcactctg ccgtggctgc gcccggacgc
    15841 gaaaagccag gtgacttttc agtatgacga cggcaaaatc gttggtatcg atgctgtcgt
    15901 gctttccact cagcactctg aagagatcga ccagaaatcg ctgcaagaag cggtaatgga
    15961 agagatcatc aagccaattc tgcccgctga atggctgact tctgccacca aattcttcat
    16021 caacccgacc ggtcgtttcg ttatcggtgg cccaatgggt gactgcggtc tgactggtcg
    16081 taaaattatc gttgatacct acggcggcat ggcgcgtcac ggtggcggtg cattctctgg
    16141 taaagatcca tcaaaagtgg accgttccgc agcctacgca gcacgttatg tcgcgaaaaa
    16201 catcgttgct gctggcctgg ccgatcgttg tgaaattcag gtttcctacg caatcggcgt
    16261 ggctgaaccg acctccatca tggtagaaac tttcggtact gagaaagtgc cttctgaaca
    16321 actgaccctg ctggtacgtg agttcttcga cctgcgccca tacggtctga ttcagatgct
    16381 ggatctgctg cacccgatct acaaagaaac cgcagcatac ggtcactttg gtcgtgaaca
    16441 tttcccgtgg gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg gtctgaagta
    16501 atctttcttc
//
LOCUS       DAPOZO010000101        16504 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPOZO010000101 DAPOZO010000000
VERSION     DAPOZO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.101"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..54
                     /locus_tag="ROI21_003761"
     ncRNA           <1..54
                     /ncRNA_class="other"
                     /locus_tag="ROI21_003761"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            101..232
                     /locus_tag="ROI21_003762"
     ncRNA           101..232
                     /ncRNA_class="other"
                     /locus_tag="ROI21_003762"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(417..1094)
                     /gene="narI"
                     /locus_tag="ROI21_003763"
     CDS             complement(417..1094)
                     /gene="narI"
                     /locus_tag="ROI21_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDX5112634.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1094..1804)
                     /gene="narJ"
                     /locus_tag="ROI21_003764"
     CDS             complement(1094..1804)
                     /gene="narJ"
                     /locus_tag="ROI21_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDX5112635.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLIDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1801..3339)
                     /gene="narH"
                     /locus_tag="ROI21_003765"
     CDS             complement(1801..3339)
                     /gene="narH"
                     /locus_tag="ROI21_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDX5112636.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3336..7079)
                     /locus_tag="ROI21_003766"
     CDS             complement(3336..7079)
                     /locus_tag="ROI21_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDX5112637.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLLGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     ILLHKLPVKRLQLADGRTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TADELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVEWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPVGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7474..8865)
                     /gene="narK"
                     /locus_tag="ROI21_003767"
     CDS             complement(7474..8865)
                     /gene="narK"
                     /locus_tag="ROI21_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDX5112638.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGSELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9204..11000
                     /gene="narX"
                     /locus_tag="ROI21_003768"
     CDS             9204..11000
                     /gene="narX"
                     /locus_tag="ROI21_003768"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDX5112639.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLNYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10993..11643
                     /gene="narL"
                     /locus_tag="ROI21_003769"
     CDS             10993..11643
                     /gene="narL"
                     /locus_tag="ROI21_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDX5112640.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11644..13038)
                     /gene="ychO"
                     /locus_tag="ROI21_003770"
     CDS             complement(11644..13038)
                     /gene="ychO"
                     /locus_tag="ROI21_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDX5112641.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKLDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDYLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSVEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            13224..13577
                     /gene="ychN"
                     /locus_tag="ROI21_003771"
     CDS             13224..13577
                     /gene="ychN"
                     /locus_tag="ROI21_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDX5112642.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13621..14316)
                     /gene="chaC"
                     /locus_tag="ROI21_003772"
     CDS             complement(13621..14316)
                     /gene="chaC"
                     /locus_tag="ROI21_003772"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDX5112643.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14474..14704)
                     /gene="chaB"
                     /locus_tag="ROI21_003773"
     CDS             complement(14474..14704)
                     /gene="chaB"
                     /locus_tag="ROI21_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDX5112644.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14974..16074
                     /gene="chaA"
                     /locus_tag="ROI21_003774"
     CDS             14974..16074
                     /gene="chaA"
                     /locus_tag="ROI21_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDX5112645.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIGINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         3994 a         4452 c         4132 g         3926 t
ORIGIN      
        1 caacgacccg aagggcgaag cgtgcagcgc tgagtaatcc ttcccccacc accatcactt
       61 tcaaaagtcc gtgaactctc aaacgaatcc gcaattagat attctgccca tgcggggaag
      121 gatgagaagc ttcgaccaag gtgcgactcg agcgccagcg agaaagcgtt gccgcaggca
      181 acgacccgaa gggtgaagcg cgcagcgctg agtaatcctt cccccaccac catcacttcc
      241 tgtaaatact caaaacctgt tactccgagc taattctacc caatggcacc gcgtttaccg
      301 gacctacaat atcctcccct gaagttcgga taagcgaagc gcatccgaca actcaccccg
      361 taccgtgccc ataaaaaaac cccgccgaag cggggtctat gcaaactaaa attcgcttag
      421 tgacgagcgc gcaccagctg atacttacgt gtcaaatact ccaccggtac gctccagatg
      481 tgcaccagac gtgagaacgg gaacagcaag aataacgtca tccccagcac caggtgcaga
      541 cggaagataa acgccacacc atcaaggtgt tgagaagcgc caccgtggaa ggtcactacc
      601 gactgcgccc agccaaccag tttcatcatc tcgctaccgt ccatatgctg ggcggagaac
      661 ggaatggtca gcaagcccag cgcgcactgg ataacgagca gcgacaggat caggatatcc
      721 gctccggtag tggttgcacg cacgcgtggg ctgaacagac gacgcttcag caacagcacg
      781 ccgccaatca gacacagcac accgctggca ccaccagcaa acatcgccat tttctgtttc
      841 acttcaatcg gcagccaagc ttcatacatc cagtgcggcg tcagcatacc gaagaagtga
      901 ccaacaaaaa tccccagaat accgatatgg aacaggttcg acgccaggtt catccctttg
      961 cgatccagca tctggctgga cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc
     1021 cagctaccaa tcaggaagac cgccccggct atgtacgggt agatatcaaa gaagaacata
     1081 ttcaggaatt gcattagtgc tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg
     1141 caccagcaaa gcgacgctga tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa
     1201 agaatttaac ctgctcttct tcccagacag catccagcgc ctgcggcgta tcgtcgcgcg
     1261 cttcgtcggc aattttttcc gccactttgt cgctgtcgat tgcggtattc gccagtttca
     1321 gcagcagatc aaacagcacg gcataacggc tttcacgctg ttgcagacgc gcactcagca
     1381 atgccagaat cggcgcaata tctatcaaac cctccacggc ttcgctttgc ggcagctgcg
     1441 ccaggtactc cagatacagc ggcagatggt cgggcaattc gcggctgttt aactgcaaac
     1501 cgtgctgctc atactgcgcc agcaggtcta ccatcgcctg accgcggtcg cgggattcac
     1561 cgtgcacatg ttcaaacagc aacagtgacg tggcacgacc acggtcgaac agttcgctgt
     1621 actgggcctg agcatcgagc ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg
     1681 catgggcatc ttctttcgac agatttttcg acgcggcaat cgcctcaaac atctcttgtt
     1741 gatgctgcca taaggcagca tccggatatt caaggagacg cgatacaatc acgagttcga
     1801 tcatgggtgc ggctccgttt tgctggttac gtcgatggca tcgatacggc ggctgttgaa
     1861 cagattgaat ttggtatctg aaccgtggca gccatcacca aaggtaaagc cgcagccatt
     1921 tttctccggg aaggcttccc gcgccagttc acgatgacta ctcggcacca caaagcgatc
     1981 ttcgtagtta gcaatcgcca gataacggta catctcctgt gcctgggctt cggtcagacc
     2041 gacctcttcc agcgcacggg tatccacttt cccgtcaacg gtttcagcac gtttataatg
     2101 acgcatcgcc agcatacgtt tcagcgcgcg cagtaccggt ttggtatcac cggcggtcag
     2161 cagattcgcc agatactgca ccggaatgcg caggctttcg acgtctggca gaatgccgtt
     2221 gctacccagc tcacccgcgt ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca
     2281 gaccatcggc agtgtgcgat attctggatg cagcggcagc gccagtttcc attccattgc
     2341 cattttataa accggcgact gctgtgcagc ttcaataacg ctcagcggaa taccgtcttt
     2401 aatcgcctgc tcgatgactt tcggatcgtt cggatcgagg aacacctcca gctgacgctg
     2461 gtaaagatct ttctcgttct cggtgctggc tgcacgttca atggcgtccg catcgtacaa
     2521 cagcacgccg agataacgga tacgaccgac acaggtttca gagcacacgg tcggctgacc
     2581 cgcttcaata cgcggatagc agaagatgca cttctcagac ttaccgctct tccagttgaa
     2641 atagattttt ttgtacgggc atccggtgat gcacatacgc cagccacggc atttatcctg
     2701 gtcgatcagg acgatgccat cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc
     2761 cacacatgcc gggttcaggc agtgttcgca caggcgcggc aaatacatca tgaaggtgtt
     2821 ttcgaactgg ctatacatcg ccttctggat gttgtcgaag ttcttgtctt tcgccagttt
     2881 gtcaaactca ccgcccaggt catcttccca gttcggccct ttttcgattt tcgccatccg
     2941 ttccccggta atcagcgaac gcggacgggc aatcggctgc gatttgctgc cttccggcgc
     3001 ggtatgcagg ttctgatagt caaaatcgaa cggctcgtaa taatcgtcga tccccggcag
     3061 atgcgggtta gcgaagattt tacccagcag catggcacgg ttacccatgc gcggctgcag
     3121 tttgccgttg attttacgaa tccagccgcc tttgtatttt tcctggtttt cccagtcagt
     3181 cgggaagccc tggcccggct tggtttccac gttgttgaac cacgcgtatt ccacgccttc
     3241 gcggctggtc cagacgtttt tacaggtaac tgaacaggtg tggcagccga tgcacttatc
     3301 gagattcagc accatgccga cttgtgaacg aattttcatt ttacgctctc ctgtacctgg
     3361 tcattgcctt cgccatctaa ccagtcaatg ttcttcatct tacgcactac aacaaactca
     3421 tcgcggttag aacctacggt gccgtagtag ttaaagccgt atgccagatg ggcatagccg
     3481 ccaatcatat gcgtcggttt cggcgtgata cgggtgaccg agttatggat accaccacgc
     3541 tgttgggtaa tttccgaacc cggcaggtta acgatacgtt cctgcgcgtg gtacatcatg
     3601 gtcatcccta ccggaacacg ctggctgaca accgcacggg cagtcagagc accgttgctg
     3661 ttgaagactt caatccagtc gttatcggca atgcccagat ctttggcatc ggcttcactc
     3721 agccagacca ccggaccacc gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg
     3781 gagtggatac cccacttctg gtgcggcgtc aggaagttga gcgctttttc cgggttgccg
     3841 ttggatttct ggcctatcac ttctttcacc gaacgggtgt cgatcggcgg acgataaacc
     3901 agcaggcttt caccgaaatc acgcatccac tggtgatcct gatacagctg ctgacggcca
     3961 gagagcgtac gccacgggat cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg
     4021 tgttcatctt caaggccaga ccaggtcggg ctggagatga ttttgcgcgg ctgtgcctga
     4081 atatcgcgga agcgaatctt ctcgtcttct ttattcagcg ccagatgcgt atggtcacga
     4141 ccggtaaact cgctcagggc agcccaggct ttcacggcta cctgaccgtt ggtttccggt
     4201 gccagcgtca ggatcatctc tgccgcatca atggcggtgt tcagcatcgg ctgacctttc
     4261 gccggacctt ccgctttggt gtagttgagc ttacgcagca gatccatctc gctctgggtg
     4321 ttccaggcaa tccctttacc gccattaccg attttctcca tcagtgggcc gatagaggta
     4381 aagcgttcgt aagttgccgg ataatcgcgc tctacgacca taatgtgcgg tgcagtttta
     4441 cctgggatca ggtcgcattc gccttttttc cagtctttca catccagcgg ttgcgccagt
     4501 tcagcggcag agtcgtgctg gataggcagc gtgacgatgt cagtttcttt acccagatga
     4561 ccaacgcaca cttcggagaa tttcttcgcg atggctttgt agatttccca gtcgcttttc
     4621 gcttcccagg ccggatcgac cgccgcagac agcgggtgaa taaacggatg catatccgaa
     4681 gtattcatgt cgtctttttc gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga
     4741 caggtgctcg acagacggaa gtccagcgtc accaccagat ccagcttgcc ttccagaccg
     4801 ttgtcctgcc attccacttc ttccggcttc acgccgccct gctgccccag gtctttgccc
     4861 tggataccgt gctccgtccc cagcaggtac ttgagcataa actcatgacc tttaccggaa
     4921 gaaccgagca ggttagaacg ccagatgaac aggttacgcg ggtggttttt accgttttct
     4981 ggttgttctg ccgcaaaacg gatagaaccc tctttcaggg atttcacggt gtaatccacc
     5041 ggattcatgc ccgctttttc cgcttcgcga gcgatagtta gcgggttagt gcctaactgc
     5101 ggcgcagacg gcagccagcc catacgttct gcacggacgt taaagtcgat cagatgtccg
     5161 gtataacggg atttatccgc catcggcgac agcaactcgt ctgcggtcac ggtttcataa
     5221 cgccactggc tggagtggtt ataaaaataa gaggtgctgt tcatgtgacg cgccggacgc
     5281 tgccagtcga gggcaaacgc cagcggctgc cagccggttt gcggacgcag tttttcctga
     5341 cctacatagt gcgcccagcc acccccgctc tgaccgacac agccgcagaa aatcagcatg
     5401 ttgatcagac cacgatagtt catatcgagg tgataccagt ggttcagccc cgcaccgacg
     5461 ataatcatcg aacgaccgtg cgttttatcc gcgttatcgg caaattcacg ggcgatgcga
     5521 ataatctggc tgcgagaaac gccggtaatc tgctcggccc acgcaggggt gtaagctttc
     5581 acatcgtcgt agctggttgc gcagttaacg tcgttcaggc cacgttccag accatagttt
     5641 gccagcgtca gatcgtaaac ggtggtcacc agagcggtgc ggccatcagc cagttgcagg
     5701 cgtttcaccg gcagcttgtg cagcagaatg ttttccagtt ccactttgtt gaagtgttca
     5761 gtgccgtcgc caccaaagta cgggaagccc acctcagcga tctcatcctg gctacccagc
     5821 aggctcagtt gcagctcggt ttcttcgcca gttttgccgt cgcgctgctc aagattccat
     5881 ttgcccttct cgccccagcg gaagccaata gaaccgttcg gcgcaaccat ttcgccattg
     5941 gtattaaagg cgacagtttt ccattccgga ttgttttcct ggcccagcgc atccaccaga
     6001 tcggcagcgc gcagcatacg acccgcagcg tagtaaccgt cgcgttcttc cagcatcacc
     6061 agcatcggca tgtcggtgta gcgacgcaca tagtcggtga aatactggct tgggttgtcg
     6121 aggtggaatt cacgcagcat tacgtggccc atcgccagcg ccattgctgc gtcggtgccc
     6181 tgtttcggtg ccagccacag atcgcacagt ttggcgattt cagcgtagtc cggtgtgacg
     6241 gcaacagttt tggtgccttt gtaacgcact tcagtaaaga agtgagcatc cggagtacgc
     6301 gtctgcggca cgtttgaccc ccaggcgatg atgtagctgg agttgtacca gtcagcagat
     6361 tccggtacgt cagtctgctc gccccaggtt tgcggagacg caggaggcag gtcgcagtac
     6421 cagtcgtaga agcttaagca agtaccgcca agcagcgaga gatagcgcgc acccgacgcg
     6481 taagaaacca tcgacattgc cggaattggc gagaagccag cgacacggtc cgggccgtag
     6541 tttttgatgg tgtaaacgtt agaggcggcg atcagttcgt tcacttcctg ccaggaagaa
     6601 cgaacaaaac caccgcgtcc acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca
     6661 atgatagaag cccatgcctc aaccggatcg ctatgcagcg ccttcgcttc acgccacatt
     6721 ttcatcaggc gtttgcgcat cattgggtat ttcaggcggt tggcgctgta aagataccag
     6781 gagtagctgg caccgcgcgg gcagccgcga ggttcatggt ttggcaggtc cgggcgagta
     6841 cgcggatagt cagtctgctg ggtttcccag gtcaccagac cgtttttgac gtagattttc
     6901 cagctgcagg agccggtgca gtttaccccg tgggtagagc ggacgatttt gtcatgctgc
     6961 caacgctggc gatatccatc ctcccagtca cggttggtat tgagaagctg gccatgccca
     7021 tcggcaaagg tttcaccctt ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc
     7081 ggttttctcc tgtgggagcc tgtcggccct ctgataaatc gacattgcta atgtttaagg
     7141 gcaaggtaac gctctgaaac agcgtgggaa ttgataacga tcaagagtga tggggatgaa
     7201 aaataaagta aattcctaag ataccccttt aggataatta acagccacca cataacagac
     7261 tgtaaattaa gacgttttat acctgtagtt atttaaacta ttaagatttt tatgcagatg
     7321 ggtattaagg agtattcccc atgggtaaca tattaatggc tcttaatgaa atccggcgaa
     7381 ataagccact cacacataag tatgttgtaa ctaaacgaca gataaaaaaa agcgcgacat
     7441 catgccgcgc aaaggataat caaatattaa gatttatttt ttagaatgcc gaccatatac
     7501 cgcccaggta atcactacgc aggcgatata gaaaatcaaa aatactttca ttgcgccgac
     7561 tggagaaccc gttaatgcca gcgagctacc gaacgctttc gggataaaga agccaccaat
     7621 cgcgccaatc gcagagatga aacccagcgc cgccgccgtg tcggttgccg cttcacgcat
     7681 cgcacgttcg tcagaacccc cttctgcttt cacgcgatcc attgtcagtt tacggaagat
     7741 cacggaaatc atctggaaag tggaaccact acccagccca gctgtcagga acagcgccag
     7801 gaagaccgcg aagaacgcca tgaagcttcc gccctgtccg tcagtcggta aggtcaggaa
     7861 cagcaggccg ctgaaaatcg ccatcaggat aaagttcacc agtgtgacac gagttccgcc
     7921 cagacggtca gataatgcac cacctgcaga acgcgccagc gcaccaataa acggcccgaa
     7981 gaaagcgtat tgcagaatct gaacatccgg gaactgcgtt tttgacagca tcgcaaagcc
     8041 cgcggagaag ccgatgaagg agccgaaggt tgccagatac agcaggctca taatccacag
     8101 atgaccccgt ttgagtaccg gcaactgctc cttgatggag gctttcgagg tagcaagatc
     8161 gttcatgcca aaccacgccg caatggtgaa gatggcaagg aacggcaccc atacccagga
     8221 cgcattcgcc agatacagct cactcccatc cggctgtttg acaccctggc taccaaatac
     8281 tgcgaaaatc gacagtgata ccaccagcgg agcaaccaac tgcatgacgc tgacgcccat
     8341 gttgcccaga ccaccattca gacccagcgc gccaccctgc ttctgtttcg gaaagaagaa
     8401 gctgatgttt gccatactgg aagcgaagtt cgcgccagca aagccgcaca gcagagagat
     8461 gatgatgaag acgctataag gcgtggaggt atcctgcacg gcaaaaccca gccagacgca
     8521 aggaataatc agaataccgg tgctgaacgc cgtccagcga cgaccaccga agataggaac
     8581 cataaaggag tatggaacac gtaataacgc gccagaaacc gaaggcagcg cagtcaacat
     8641 aaatagctga tcggtcgtaa aattaaaacc gactttcggt aagttcaccg caacagcgct
     8701 gaacaacatc catacgcaaa acgccagcag cagacaggga acggaaatcc acaggttgcg
     8761 gctggcaata cgttgaccgc gttgttgcca gaacgcagga tcttccggtc gccaatctgt
     8821 aatgacagct ccagtagccc tttcgggggc ggatgagtga ctcatagaca cctctgatac
     8881 tcgtttcgat gtctgccacc ttagtgtctg tagctaaagg caatttgatg taaatcaaac
     8941 gataagataa ctttatctat cattgatatt tatcattacc catagtgagt acagtgattt
     9001 cataaaaatt atgagatttt tcacggtgct gtaaaatccc tacccttacc gatgtaaagc
     9061 gactaaccac gcggcaaata aggagtaact ctttccgggt atgggtatac ttcagccaat
     9121 agccgagaat actgccattc cagaatgtat cgtcacattc attaaggtta ttgctcattt
     9181 aaagcctgaa ggaagaggtt tacatgctta aacgttgtct ctctccgctc accctggtta
     9241 atcaggttgc gcttattgtg ttgctttcta ctgctattgg actggcaggg atggcggttt
     9301 ctggctggct ggtgcaaggc gttcagggta gcgcccatgc gatcaacaaa gcgggatcgc
     9361 tgcgcatgca aagttaccgt ctgttggcag cagtgccatt aagcgagaaa gacaagccct
     9421 taattaaaga gatggaacaa acagcattta gcgccgaatt aacccgagca gcagaacgag
     9481 acggacaact ggcgcaatta cagggtttac aagattactg gcgtaatgaa ctgatccctg
     9541 cgctgatgcg tgcacaaaac cgcgaaacgg tgtcagcgga tgtcagccag tttgttgccg
     9601 ggcttgatca gctggtatct ggttttgacc gcaccacgga aatgcgcatt gagacagtgg
     9661 tactggtcca tcgggtaatg gcggtattta tggcactttt actggtgttc actattatct
     9721 ggttgcgggc gcgactgcta caaccgtggc ggcaattgct ggcaatggcg agtgccgtca
     9781 gtcatcgcga ttttacccaa cgcgcgaaca tcagcgggcg caacgaaatg gcgatgcttg
     9841 gaactgcgtt gaacaatatg tctgcagaac tggccgaaag ttatgccgta cttgagcagc
     9901 gggttcagga gaaaaccgcc gggctggagc ataaaaatca gatcctctct tttttatggc
     9961 aggccaaccg ccgattgcat tcccgcgccc cgctgtgtga acgcctgtca cctgtactca
    10021 acggcttaca gaatttgacc ctgctacgtg atattgaact gcgggtgtat gacactgatg
    10081 atgaagagaa tcatcaggag tttacctgcc agccagatat gacttgtgat gataaaggct
    10141 gccagctctg cccgcgcggc gtattacccg ttggcgatcg cggcacaacc ctgaagtggc
    10201 ggctggctga ctctcatacg cagtacggca ttttgctggc gaccctgccg caggggcgtc
    10261 atcttagcca tgatcaacaa caactggtgg ataccctggt tgaacaactc accgccacgc
    10321 tggcgttgga tcgccatcag gaacgtcagc aacagttgat cgtgatggaa gagcgtgcca
    10381 ccattgcgcg cgaactgcat gattctattg cccaatctct ctcttgcatg aagatgcagg
    10441 tgagttgttt acagatgcag ggcgatgcgc tgccagaaag cagtcgcgaa ctgttaagtc
    10501 agatccgtaa cgaactgaat gcatcctggg cgcagttgcg tgaattgctc accacattcc
    10561 gtttgcagct caccgagcct ggattacgtc cggcgctgga ggcgagttgc gaagagtaca
    10621 gcgccaaatt tggcttcccg gtgaagctga attatcaatt gccgcctcgt ctggtgcctt
    10681 cgcatcaggc aatccacttg ttgcaaattg cccgtgaggc attaagtaac gccctcaaac
    10741 attcgcaagc gagtgaagtc gtggtgacgg tggcgcaaaa cgataatcag gtcaaactga
    10801 ccgtccagga taacggctgc ggcgtgcctg aaaatgccat ccgcagcaat cactacggca
    10861 tgataataat gcgcgaccgt gcgcaaagtt tacgaggcga ttgccgcgtc cgccgtcgtg
    10921 aatcaggtgg caccgaagtg gtggtcacct ttattcccga aaaaactttc acagacgtcc
    10981 aaggagatac ccatgagtaa tcaggaaccg gctactatcc tgctgatcga cgatcacccg
    11041 atgctgcgaa ctggcgtaaa acagcttatc agtatggcac cagatatcac cgtggttggc
    11101 gaagcgagta atggcgaaca gggtattgaa ctggcggaat ctcttgatcc cgatttgatc
    11161 ctgttagatc tcaatatgcc cggcatgaac ggtctggaaa cgctggataa actgcgcgaa
    11221 aagtccctct ccgggcgcat tgtggtattc agcgtttcta accatgaaga agatgtggtc
    11281 accgcactga aacgcggcgc ggatggctat ctgttaaaag atatggaacc ggaagatctg
    11341 ctgaaagcgt tacatcaggc agctgctggc gaaatggtat taagcgaagc attaacgcct
    11401 gttctggccg ccagcttgcg tgctaaccgt gccactactg agcgcgatgt taaccagtta
    11461 accccacgcg agcgcgatat tctcaagctg attgcccagg gtttgccgaa caagatgatt
    11521 gcccgccgcc tggacatcac cgaaagcaca gtaaaagtgc acgtcaagca catgctgaag
    11581 aaaatgaagc tcaagtctcg cgtggaagca gcggtatggg tgcatcagga gcgcattttc
    11641 tgattacggt tcccagcgca gttcgtcgtt tgacaatgca tcgaacggtt cgacaagcgt
    11701 tagcgtgatc tcattggagg agacacgctg cccctggtta tcttccacca ccaccgacaa
    11761 tcgccagtga ttgctcgccc cttccccgtt ctgccagtca ggcatgatca gcgtccagcc
    11821 ctccacgctg ttggcttgtg cgcccggcgt caaacttaat atctgcgtat cgccctgcca
    11881 aatcagttgc cgaataccgt aacgactgcg gatttgtaat ttcagcggca ctgtttcgcc
    11941 aggttttaga tcccacggag gtgtcgccag aaacaccgtt aacgtttttc gctgtcggta
    12001 ctcaagagtc ggtagattat ttcgctgcgg attgtcatag cgactaccac gtaacgactg
    12061 actttcggca acctcgcccg cagaaagttg ttttttgagc ggtacaccaa agcggtaatt
    12121 aagattcagc ccgaggttat tttgattttc gccactttca ccctgtttat gctgggccgt
    12181 cacagtgact aatggcactg gggtgtaatt taatcccaga ctcaacgcga cgggattgtg
    12241 ataacccgta ccagagttaa acaaatcaac acgatcacca aaatactgtt ctacgctgac
    12301 actggtattg aggtgttgat agaacggcat gcgcatccgg gctgtcaggt cgtacccgcg
    12361 cgccatccgt tgttcctgcg tggctgtctg ttcgtgccat gcagcaaacg gctggtaaaa
    12421 gtttgccgat agtcgcaaat attcgcccca cgcttcggca ccaaagcccg ctcgctgaag
    12481 attttcgtcc agcaagtagt cataaaaagt gttataaccc accagccagc tgccgcgcgc
    12541 ccagcgttgc ccaacgccca cattgctcac caacccatca tcctgctgag taagacccag
    12601 ctggctccag gtgagataac gatcattatc ttgtaacggc acaaaccagc ttccacgact
    12661 gccggtgaaa tgtccttcgt tatccagttt gacgtcaaca ctggcatttc cccacggtga
    12721 tagccaggac tctacgtgct gattaacctg ttgactaagc gcatcgcgga cttttcccaa
    12781 tgcgaaagct tttgcctgct cgccagtatc cagcccgtta tcattcatac tggtttcgcc
    12841 gaaatctttc acaatttcag caaagtgttt ttccccatca tgattttcag gtgccatgcc
    12901 taaatccggc agaccatcat tgttattatc aaagggattt gccgcttttt gctcgaaggt
    12961 agattgtgcg ttagctgtac cgcctgccgc cagcaagagt aaataaaacg gaataatgcg
    13021 aggaacgaaa cggctcaaaa cgtcggtaac ttccggtaaa aaatgaaaaa ccatagccct
    13081 aaagaataac gattactggc aacttttgtc gtattttctc gcgctttcag gaaaatcctt
    13141 aaaaatgtga ccgattggtc agacgctgca gaagcagtaa gatagaagat gagtgtctaa
    13201 ttccctgaat acaggagtaa ataatgcaaa aaatcgtgat cgttgccaat ggcgcacctt
    13261 acgggagcga atccttgttt aacagcttgc ggctggccat tgcgttacga gagcaggaga
    13321 acaatctgga tctgcgtctg ttcctgatgt ctgatgcggt cacagccggg ttgcgcgggc
    13381 aaaaaccagg ggaaggctac aacattcagc aaatgctgga gatccttacc gctcagaatg
    13441 taccggtgaa attgtgcaaa acctgtacag acgggcgcgg gattagcaca cttcctctga
    13501 ttgatggggt ggaaatcggt actcttgtgg aactggcgca atggacgctg tcagccgata
    13561 aagtgctcac attttaatgt ttgagggggc gtctgacgcc ccactatgag ggaagtttac
    13621 tcaggcgaat cccggccgta acacaccatc cggataattc tccgccagca gtttttttac
    13681 cgataccagc aaatcattca gcccatcgtc ctgcattccc agtttgataa gctcctgttc
    13741 cagtgaaaac aggtattgtg cgttggttcc cagtggaccg ctcgccgccg caatcaacgg
    13801 cgcgatgacc tgagcgcgag tgtcagattc atattctgga tgtcgcgggt ccataataaa
    13861 cacaatggcg tttactgtgc gtccatcatc aagatcaagc tgacaccagg ttggcagata
    13921 acagccggta atcatctcgc gcttccacaa cagggttagt tcctgctcca gcgtctcttc
    13981 tggcagtcga taggcgacgc ctgtggtgcg tccgccctct ttcagtgcaa gcattcgtcc
    14041 cggctggtgc gcagtcccac gcccggcggt cagacgcagg caaaacgcac gatgccatcc
    14101 aaccagtgta ccggtgcacg actcggtaaa ctccagtgcc ggattccaca tcaacgatcc
    14161 ataaccgaag atccacaccg gtccctcatc aggtcgacaa gccagcgtcg ccgccagcga
    14221 agccgcccgt tgctctgctg accataagag tgattcttca atggcaccaa atgccgtttt
    14281 acaatcggca ttcatcaaga aatcacgcgt tatcacctta cacctccgcc actgcatact
    14341 tccctgcgac aactttttgg tgtcttccgg acatttttac tgctcatttt gaaaccacta
    14401 acgggacaat atgcaatcca cggaccactt gcaaggcaac accgcacgaa ttgctttaac
    14461 taagctaccg gttttacgat tttttatgcc atttatcatc atcccctttg gcatattcat
    14521 gcttcacagc agcccaggcc actttatgcg cggtttcttc gcgactggcg tcatcacgcc
    14581 gatcttcttt atctttatat tgatcccatg cgctgttgaa cgcttcttta tagatatcct
    14641 gagcatgaga cggtagcacg tgctttacgc tttccggcag atcgcttttc gttttatacg
    14701 gcatcgttga ctccttttac aaagttgaac attaagtgtg gtcaacaata tccactcttg
    14761 ccaaaacata agaattttca tgtcagactc ttcttaagaa acgctaaaac aaacattgtc
    14821 taaacgcatt gcttattttt atggcccgat gagtaaaaaa tcgacagcgg ggtaaaaatt
    14881 cgtaaaatgt taccgatatc cagacacttt ctgttatttt tcctacctat tttttacatt
    14941 ttcgataaat tcatctgcaa agggataacc ataatgtcaa atgctcaaga ggcggtaaaa
    15001 acccgccaca aggagacttc gcttattttc ccggttctgg cgctggtagt gctgttcctg
    15061 tggggaagca gccagacact accagtggtc attggcatca atcttcttgc tcttattggt
    15121 attttaagta gcgcctttag tgttgtccgt catgcggacg tattagccca tcgcctggga
    15181 gaaccttacg gttcgcttat tcttagcctt tcagtggtta ttcttgaagt cagtttgatt
    15241 tcagctttaa tggcaaccgg cgacgccgcg ccaacgctaa tgcgtgatac gctctattca
    15301 atcattatga ttgttaccgg tgggctggtt ggcttttcat tattgttggg cggacgcaag
    15361 tttgccaccc aatatatgaa tctgtttggt atcaagcagt atttaattgc gctgttcccc
    15421 ctggcgataa tcgtactggt atttccaatg gctctgcctg cggcgaattt ttcaaccggt
    15481 caggcgttac tggtagcatt aatttctgcg gcaatgtatg gcgtattttt gctgatccag
    15541 accaaaacgc atcaaagttt atttgtctac gagcacgaag atgacagtga tgatgacgac
    15601 ccgcaccacg gtaaaccgtc tgcccatagc agcctttggc atgctatctg gttgattgtc
    15661 catctgattg ccgttattgc agtcaccaaa atgaacgcca gcccgctgga gacattgctc
    15721 gacagcatga atgcccctgt cgcctttact ggcttcctgg tggctctgtt gattctgtcg
    15781 ccggaaggtt taggtgcgtt aaaagcagtg ttgaacaacc aggtccagcg cgcgatgaat
    15841 ctgttctttg gttcagtgtt agcaaccatt tcgcttaccg tacctgtcgt cacgctaatt
    15901 gcctttatga cgggtaacga attgcagttt gcacttggtg cgccagaaat ggtagtgatg
    15961 gtggcctctt tagtgctgtg ccatatctcc ttctccaccg gacgtactaa cgtgctcaat
    16021 ggcgcagcgc atctggcact gtttgccgcc tatttgatga cgatatttgc ctgacagtta
    16081 tatctcctcc gatgacagaa atcgggggag ataatataaa ttcctgctta ttgtatttct
    16141 tcatactacc tgaacaggtt gttaatcaga attgcgagcc tgtaccagaa cctgcacgaa
    16201 accactgcaa cagctgttga aaagccggaa tgtactcttt attgcaaaca tttcagggat
    16261 gaagtgaaga tgaattgaga cgtagtatcc acaccaagcc ggcgttatcc ggtgaggcgc
    16321 aatgttgcgg gggctttatc cctggtggca ttggttgctg gaaagagaaa acccccgcac
    16381 gttgagaggt aaaacctgac aacagaacgg gggctaatct tgaaccatca acaattcaag
    16441 aatagccgca aaatgttgtc attacaacaa ggcggctata tgacgctcgc gcagtttgcc
    16501 atga
//
LOCUS       DAPOZO010000102        16411 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPOZO010000102 DAPOZO010000000
VERSION     DAPOZO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.102"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(3..1283)
                     /gene="hemL"
                     /locus_tag="ROI21_003775"
     CDS             complement(3..1283)
                     /gene="hemL"
                     /locus_tag="ROI21_003775"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDX5112646.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQAGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAGSVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            1508..2929
                     /gene="clcA"
                     /locus_tag="ROI21_003776"
     CDS             1508..2929
                     /gene="clcA"
                     /locus_tag="ROI21_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDX5112647.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            2953..3018
                     /locus_tag="ROI21_003777"
     CDS             2953..3018
                     /locus_tag="ROI21_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112648.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            3011..3355
                     /gene="erpA"
                     /locus_tag="ROI21_003778"
     CDS             3011..3355
                     /gene="erpA"
                     /locus_tag="ROI21_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDX5112649.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3402..4025)
                     /locus_tag="ROI21_003779"
     CDS             complement(3402..4025)
                     /locus_tag="ROI21_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDX5112650.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(4063..4863)
                     /gene="btuF"
                     /locus_tag="ROI21_003780"
     CDS             complement(4063..4863)
                     /gene="btuF"
                     /locus_tag="ROI21_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDX5112651.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(4856..5554)
                     /gene="mtnN"
                     /locus_tag="ROI21_003781"
     CDS             complement(4856..5554)
                     /gene="mtnN"
                     /locus_tag="ROI21_003781"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDX5112652.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            5638..7155
                     /gene="dgt"
                     /locus_tag="ROI21_003782"
     CDS             5638..7155
                     /gene="dgt"
                     /locus_tag="ROI21_003782"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDX5112653.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLEEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHYYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            7285..8709
                     /gene="degP"
                     /locus_tag="ROI21_003783"
     CDS             7285..8709
                     /gene="degP"
                     /locus_tag="ROI21_003783"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDX5112654.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKI
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            8864..10021
                     /gene="cdaR"
                     /locus_tag="ROI21_003784"
     CDS             8864..10021
                     /gene="cdaR"
                     /locus_tag="ROI21_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDX5112655.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(10075..10461)
                     /locus_tag="ROI21_003785"
     CDS             complement(10075..10461)
                     /locus_tag="ROI21_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDX5112656.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(10775..11599)
                     /gene="dapD"
                     /locus_tag="ROI21_003786"
     CDS             complement(10775..11599)
                     /gene="dapD"
                     /locus_tag="ROI21_003786"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDX5112657.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(11630..14302)
                     /gene="glnD"
                     /locus_tag="ROI21_003787"
     CDS             complement(11630..14302)
                     /gene="glnD"
                     /locus_tag="ROI21_003787"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDX5112658.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRHLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQSAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(14364..15158)
                     /gene="map"
                     /locus_tag="ROI21_003788"
     CDS             complement(14364..15158)
                     /gene="map"
                     /locus_tag="ROI21_003788"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDX5112659.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            15526..16251
                     /gene="rpsB"
                     /locus_tag="ROI21_003789"
     CDS             15526..16251
                     /gene="rpsB"
                     /locus_tag="ROI21_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDX5112660.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
BASE COUNT         3937 a         4141 c         4344 g         3989 t
ORIGIN      
        1 catcacaact tcgcaaacac ccgacgtgca gcatcgatgg tgttattgat atcttccatg
       61 ctgtgcgcca cagacataaa gcccgcttca aacgctgacg gtgccagata aacaccttcg
      121 tccagcatca tatggaagaa acgcttaaag cgttccacgt cacaggccat cacatcctga
      181 tagcacgtca cggacccggc gtcggtaaag aaaataccga acatgccgcc aacgtggttt
      241 accaccagcg gaattccggc ttcttctgcc gcttcccgca gaccttctgc cagacgtgtt
      301 gtcagctcat ccagcgtttc gtgaacgccc gcctgcgcga cttcattcag acaggcgaaa
      361 ccagcagcca tcgcaatcgg gttaccggaa agcgtacccg cctgatagac cggaccggtc
      421 ggggccagcg catccattac atcacgacga ccaccgaatg cgcctaccgg cattccaccg
      481 ccgatgattt tgccgaggca ggtgagatcc ggttccacac cgtaataatc ctgtgcgcca
      541 gctagcgcta cgcggaaacc ggtcatcact tcatcgatga tcagcaacgc gccaaattcg
      601 tcgcacagcg cgcgcagacc tggcaggaac tctggcagcg gtggaacgca gttcatattg
      661 cctgccaccg gctcgacgat aatacaggca atctcttgcg ggtattgctc aaacgcggcg
      721 cgtacagagg ccagatcgtt ataagtacag gttaaggtat gtttggcgaa atctgccgga
      781 acgcccggcg agtttggctg gcctaacgtg agtgcgccag aaccggcttt caccagcagg
      841 cagtcagcgt gaccgtggta acaaccttca aatttaataa ttttgtcgcg accggtaaaa
      901 ccacgggcca ggcggatggc gctcattgtc gcttcagtgc cggagttcac catgcgcacc
      961 atatccatgg tcgggaccag ttcggtcacc agttgcgcca ttttcacttc catttcggtt
     1021 ggtgcaccaa agcttaaacc acgctcggcg gcttcaatca cggcattgcg gatcgccgga
     1081 tggttatggc ccagcaccat cggcccccag gaaccgacat aatcgatata ggctttgcca
     1141 tcaacatcgt acagataagc gccgtccgct ttttcgataa acagtggagt gccgcccacg
     1201 ccagtaaagg cgcgaacagg ggagttcaca ccgccaggga tcagctcgcg cgctgcgctg
     1261 taaagatttt cagacttact catggagggt tcctgattcg tagaaaaagt gaatggctgc
     1321 tattctatgt tattcataac aagttaaata ctcgtcaaac atcaggctgc ttgtactggt
     1381 gcaatcctga tttcttagag tataaaagtt ttgtgcattt gaaacattac gctttgcaaa
     1441 ggattttcat ggaacgtgcg agtaaaatgc cgtcatctta tttgtatgac caataagtga
     1501 tcattggatg aaaactgata ctccctcttt agaaacaccg caggccgcgc gcctgcgacg
     1561 cagacaactg attcgccaac ttcttgagcg cgataaaacc ccgttagcca ttttgtttat
     1621 ggcggcagtc gtcggcacgc ttgtcgggct ggctgcggtt gcttttgaca aaggtgtcgc
     1681 ctggttgcag aaccaacgta tgggggcgct ggtacatact gctgataatt atccgcttct
     1741 gttaaccgtc gcttttctct gttcggcggt gctggcgatg ttcggctact ttttggtgcg
     1801 caaatacgcg ccggaagcag gtggttcggg gatcccggaa attgaagggg cgctggaaga
     1861 tcaacgtccc gttcgctggt ggcgtgtgtt gccggtgaaa ttctttggcg ggctgggaac
     1921 actcggcgga ggcatggtgc tgggccgcga agggccaacc gtacagatcg gcggcaatat
     1981 cggtcgcatg gtgcttgata ttttccgcct gaaaggtgat gaagcgcgcc atacgctgct
     2041 ggcaaccggt gctgcggcgg ggctggctgc ggcctttaac gccccgctgg cgggtatttt
     2101 gtttattatc gaagagatgc gtccgcagtt tcgctatacg ttaatttcga ttaaagcggt
     2161 atttattggc gtcattatgt cgaccattat gtaccggatt tttaatcatg aagttgcgtt
     2221 gattgacgtc ggtaaacttt ctgacgcgcc gcttaatacg ctgtggcttt atctgatcct
     2281 cggtattatt tttggcattt tcggccctat ttttaataaa tgggtgctgg gaatgcagga
     2341 tttgctgcac cgtgtgcacg gcggcaatat taccaaatgg gtactgatgg gcggtgcgat
     2401 tggcggtctg tgtggattgc tggggtttgt ggcaccagca acgtcgggcg gcggttttaa
     2461 cctgattcct atcgctaccg cggggaattt cagcatggga atgctggtgt ttatcttcgt
     2521 cgcgcgggtc attaccacct tgctctgctt ctcttccggc gcgccgggcg gtatttttgc
     2581 cccgatgctg gcgctgggta ctgtgctggg aaccgctttc ggaatggttg ccgttgagct
     2641 gtttccgcaa tatcaccttg aggcggggac atttgctatt gccggaatgg gggcattact
     2701 ggcggcatct attcgcgcgc cgttaacggg gatcattctg gttctggaga tgaccgataa
     2761 ctaccagctc attttgccaa tgattattac cggtcttggc gcaacactat tagcgcaatt
     2821 taccggcggg aaaccgctat actcggcgat tcttgcgcgc acgctggcaa aacaggaagc
     2881 tgagcaactg gcgcgaagca aggccgcatc agccagcgag aatacttgaa cgaaatacca
     2941 gggtattaga taatggcgat tattattggg ttagaatttg cccaattgcc gatgtcgttt
     3001 ggagcaaaat atgagtgatg acgtagcact gccgctggag tttaccgacg cagcagccaa
     3061 caaagttaaa agcctgatcg ctgacgaaga taacccgaat ctgaaattac gcgtgtatat
     3121 caccggtggc ggttgcagcg gcttccagta tggtttcacc tttgatgatc aggtgaacga
     3181 aggcgatatg accatcgaaa aacagggcgt tggcctggtg gttgatccga tgagcctgca
     3241 atatctggtc ggcggttccg ttgactatac cgaaggactg gaaggttctc gtttcatcgt
     3301 gaccaacccg aacgcgaaaa gcacctgcgg ttgcggttct tcctttagca tctaatctgt
     3361 tgttcatgtg ccggataaac tatccggcac atcttctctc ttcaacgccc attctcatcc
     3421 agcgcaaacg tcggtagctt aagatgccaa cgaatagccg ccagccgaat caatagcgtc
     3481 acgaccatgc ccatcatact ggctgtttcc agtggtacgg aaaatgtgta ataagccgta
     3541 gcgtggacaa taccgccgat aatacaggct gttgcgtaga tttctgtacg taaaatcatg
     3601 gggatttcgc gggccagtac atcacgaatg atcccgccgc caacgccagt aatgacgccc
     3661 atacaaaccg cgattaacgg accggcttcc gcattaaagg ctttattcac gccaatgccg
     3721 acaaacaccg ccagaccaac ggcgtccagc accggcaaca tccattttgg taagcgtctt
     3781 ggctggcgca ccagcacgat ggtcagcatg ctggtgacca ttgcaacgac cagatcggtg
     3841 ggatctttca cccaaaatac cgggccgtga tccagcgcca tgtcgcgaat tgtcccgccg
     3901 cctactgcgg taaccacgcc cagtaccaga acaccaaaag ggtccatacg caattttccg
     3961 gctaacaaaa cgccagagat agcaaatacc gctgtgccga ctatatccag ccaatagacg
     4021 agcattgttc aatccccact gagcacctga aaaggtcagg cgctaatcta cctgtgaaag
     4081 cgcattacag agctgttgtg cagcgaggat aatacgtggg cttgcacgtt caaaccagtc
     4141 actcgtgaga ggaataacgg gaattttgag ctgttcaccc cagtattgtt tgattttagg
     4201 aatttggtcc ggtccgcctg taatgactat cgcctgtggc gcgcgtgcta acacctgttc
     4261 gcggctaact tgcggccagg gaacccggct atctttaaag atgttttctc cgccacaaac
     4321 ttcgagtacc tggttctgaa tcgactcttt tccactggta aatggcggat taatgccgaa
     4381 ttgcagaaaa acacgttttt taggtttatc agcatattgc gctttcaatt gcgcgtactg
     4441 gtccagcagg gattgcgcgg cttgttcggc cttgtctggt tgcggactcc agggggccag
     4501 ttgacgtaac gcattggcaa tttgttcaat gcttgtcgca tcgacccaca tcacttttat
     4561 tcccagcgaa gccagctggt caacctgccg ctcggcatta cctccacgcc aggcaatcac
     4621 cagatcgggt ttcagcgcga caatgcgttc cagattcatc ccctgccagg tggaaacctg
     4681 ctcaatcttt tgcgcttgtg gaggatagtc ggaatagctg ctgaccccaa ccggcgtgat
     4741 cccggcggca aaggcgagtt cagtgttggc gggagaaagc gtgatgacgc gcggcgcggc
     4801 gttgagccac agcggcgcga gaaaagacag ggcgaccagc gccctgaaca gtgacttagc
     4861 catgtgccag tttctgtacc agtgactcaa ccatcaggct ggactgttta gcggcaacag
     4921 ccaggaactc atcgaagcta agatgagact gttgatcggc cacgtcggag atggcgcgta
     4981 ctacgacaaa cgggacgttg aaattgtggc agacatgggc gattgccgtc gcttccatct
     5041 ctacagcaat ggcctgtggg aagttgtggc ggattttcgc cagaccaaca gaaccgttga
     5101 tgaatgcgtc gccgctaaca atcaggccac gtacagcgtt aagattcagt tcggcaatgc
     5161 aggcctcagc ggcagcgatc agtttatcgt cagctttgaa gcctgccggg cagcctggta
     5221 actgaccgta ttcataacca aatgccgtga catccgcgtc gtgataacgt gcttcgtccg
     5281 agacaacgat atcgcccact ttcagcgttg gtgccaggcc accggcagaa ccggtgttaa
     5341 taatcacatc tggcttgcag tgttccaaca gcaaagtggc acccagcgcc gcagcgactt
     5401 taccgatgcc cgatttcaga agcgcaacct cggttccatt cagttggccg gtatagattt
     5461 cgcaaccgcc gagactgata gtttgacggt tttcgatttt gtcacgcagc agcgtaactt
     5521 cttcttccat tgcaccaatg atgccgattt tcatagattt actcgcgata agcccgattt
     5581 gaaggcatag tttaccatgc gcttacgggg aagcgtattt ctcacgcggg agaggacatg
     5641 gcacagattg atttccgaaa aaaaataaac tggcatcgtc gttaccgttc accgcagggc
     5701 gttaaaaccg aacatgagat tctgcggatc ttcgagagcg atcgcgggcg tatcatcaac
     5761 tctccggcaa ttcgtcgtct gcaacaaaag acccaggttt ttccactgga gcgcaatgcc
     5821 gccgtgcgca cgcgtcttac ccactcgatg gaagtccagc aggtggggcg ctacatcgcc
     5881 aaagaaattt taagccgtct gaaagagctt aaattgctgg aagcatacgg cctggaagaa
     5941 ctgaccggcc cctttgaaag cattgttgag atgtcatgcc ttatgcacga tatcggcaat
     6001 ccgccgtttg gtcattttgg cgaagcggcg ataaatgact ggtttcgcca gcgtttgcac
     6061 ccggaagatg ccgaaagcca gcctctgact gacgatcgct gcagcgtggc agcactacgt
     6121 ttacgggacg gggaagaacc gcttaacgag ctgcggcgca aaatacgtca ggacttatgt
     6181 cattttgagg ggaatgcaca aggcattcgt ctggtgcata cattgatgcg gatgaatctc
     6241 acctgggcac aggttggcgg tattttaaaa tatactcgtc cggcgtggtg gcgtggtgaa
     6301 acgcctgaga cacattacta tttaatgaaa aagccgggtt attatctttc tgaggaagcc
     6361 tatattgccc ggttgcgtaa agaacttaat ttggcgcttt acagtcgttt tccattaacg
     6421 tggattatgg aagctgccga cgacatctcc tattgtgtgg cagatcttga agatgcggta
     6481 gagaaaagaa tatttaccgt tgagcagctt tatcatcatt tgcacgaggc gtggggccag
     6541 catgagaaag gttcgctctt ttcgctggtg gttgaaaatg cctgggaaaa atcacgctca
     6601 aatagtttaa gccgcagtac ggaagatcag ttttttatgt atttacgggt aaacacccta
     6661 aataaactgg taccctacgc ggcacaacga tttattgata atctgcctgc gattttcgcc
     6721 ggaacgttta atcatgcatt attggaagat gccagcgaat gcagcgatct tctaaagtta
     6781 tataaaaatg tcgctgtaaa acatgtgttt agccatccag atgtcgagca gcttgaattg
     6841 cagggctatc gggtcattag cggattatta gagatttatc gtcctttatt aagcctgtcg
     6901 ttatcagact ttactgaact ggtagaaaaa gaacgggtga aacgtttccc tattgaatcg
     6961 cgcttattcc acaaactctc gacgcgccat cggctggcct atgtcgaggc tgtcagtaaa
     7021 ttaccgtcag attctcctga gtttccgcta tgggaatatt attaccgttg ccgcctgctg
     7081 caggattata tcagcggtat gaccgacctc tatgcctggg atgaataccg acgtctgatg
     7141 gccgtagaac aataaccagg cttttgtaaa gacgaacaat aaatttttac cttttgcaga
     7201 aactttagtt cggaacttcg cgctataaaa cgaatctgaa gaacacagca attttgcgtt
     7261 atctgttaat cgagactgaa atacatgaaa aaaaccacat tagcactgag tgcactggct
     7321 ctgagtttag gtttggcgtt atctccgctc tctgcaacgg cggctgagac ttcttcagca
     7381 acgacagccc agcagatgcc aagccttgca ccgatgctcg aaaaggtgat gccttcagtg
     7441 gtcagcatta acgtagaagg tagcacaacc gttaatacgc cgcgtatgcc gcgtaatttc
     7501 cagcagttct tcggtgatga ttctccgttc tgccaggaag gttctccgtt ccagagctct
     7561 ccgttctgcc aggggggcca gggcggtaat ggcggcggcc agcaacagaa attcatggcg
     7621 ctgggttccg gcgtcatcat tgatgccgat aaaggctatg tcgtcaccaa caaccacgtt
     7681 gttgataacg cgacggtgat taaagtccag ctgagcgatg gtcgtaagtt cgacgcgaag
     7741 atggttggca aagatccgcg ctctgatatc gcgctgatcc agatccagaa cccgaaaaat
     7801 ctgaccgcaa ttaagatggc ggattctgat gcactgcgcg tgggtgatta caccgtagca
     7861 attggtaacc cgtttggtct gggcgagacg gtaacttccg ggattgtctc tgcgctgggg
     7921 cgtagcggcc tgaatgctga aaactacgaa aacttcatcc agaccgatgc agcgatcaac
     7981 cgtggtaact ccggcggtgc gttggttaac ctgaacggcg aactgatcgg tatcaacacc
     8041 gcgatcctcg caccggacgg cggaaacatc ggtatcggtt ttgctatccc gagcaacatg
     8101 gtgaaaaacc tgacctcgca gatggtggaa tacggccagg tgaaacgtgg tgagttgggt
     8161 attatgggca ctgagctgaa ctccgatctg gcgaaagcga tgaaagttga cgcccagcgc
     8221 ggtgctttcg taagccaggt tctgccgaat tcttccgccg cgaaagcggg cattaaagcg
     8281 ggtgatgtga tcacctccct gaacggtaag ccaatcagca gctttgccgc actgcgtgct
     8341 caggtgggca ctatgccggt gggtagcaaa ctgaccctgg gcttactgcg cgacgggaag
     8401 caggttaacg tgaacctgga actgcagcag agcagccaga atcaggttga ttccagcacc
     8461 atcttcaacg gcattgaagg cgctgagatg agtaacaaag gcaaagatca gggcgtggta
     8521 gtgaacaacg tgaaaacggg cactccggct gcgcagatcg gcctgaaaaa aggtgatgtg
     8581 attattggcg cgaaccagca ggcagtgaaa aacatcgctg aactgcgtaa aattctcgat
     8641 agcaaaccat ctgttctggc gctgaacatt cagcgtggcg acagcaccat ctacctgtta
     8701 atgcagtaat ctccctcaac cccttcctga aaacgggaag gggttctcct tacaatctgt
     8761 gaacttcacc acaactccat acatcttcat catcctttgg gcatttgcac aatgccgtac
     8821 gttacgtact tccttatgct aagccgtgca taacggagga cttatggctg gctggcatct
     8881 tgataccaaa atggcgcagg atatcgtggc acgtaccatg cgcatcatcg ataccaatat
     8941 caacgtaatg gatgcccgcg ggcgaattat cggcagcggc gatcgtgagc gtattggtga
     9001 attgcacgaa ggtgcattgc tggtactttc acagggacga gtcgtcgata tcgatgacgc
     9061 ggtggcacgt catctgcacg gtgtgcggca agggattaat ctaccgttac ggctggaagg
     9121 tgaaattgtc ggcgtaattg gcctgactgg tgaaccagag aacctgcgta aatatggcga
     9181 actggtctgc atgacggctg aaatgatgct ggaacagtcg cggttgatgc acctgttggc
     9241 gcaggatagc cgtttgcggg aagaactggt gatgaacctg attcaggctg aggagaatac
     9301 ccccgcactt actgaatggg cgcaacggct ggggatcgat ctcaatcaac cgcgagtggt
     9361 ggctattgtt gaggtcgaca gcggtcagct tggcgtggac agcgcaatgg cggagttaca
     9421 gcaactgcaa aacgcgctga ctacgcctga gcgtaataat ctggtggcaa ttgtttcgct
     9481 gaccgaaatg gtggtgttga aaccggcgtt gaactctttt gggcgctggg atgcagaaga
     9541 tcatcgtaag cgagttgaac aactgattac ccgcatgaaa gagtacggcc agctgcgttt
     9601 tcgcgtttcg ctgggcaact attttaccgg accgggcagt attgcccgct cttatcgtac
     9661 ggcgaaaacg acgatggtgg tgggtaaaca gcgaatgcca gaaagtcgct gctattttta
     9721 tcaggatctg atgttgcctg tgttactcga cagtttgcgt ggcgactggc aggccaacga
     9781 actggcgcga ccgctggcgc ggctgaaagc gatggataat aacggcttgc tgcgacgtac
     9841 gctggcggcg tggtttcgcc ataacgtgca gccgctggca acgtcaaagg cgctgtttat
     9901 tcatcgtaat accctggagt atcggcttaa tcgcatatcg gaactgaccg ggctggattt
     9961 gggcaatttt gatgacaggt tgctgctgta tgtcgcgttg caactggatg aagagcggta
    10021 ggtcgatgac aaaattacag ccgggtgaga ccttacccgg cccacagaat ggcattattt
    10081 acgcgtcagt ttttccagat cggcttcaat ctcgctgatc ttattggtta ctactgactc
    10141 caggtgacgt aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg
    10201 gcagatttga tctaactcat caatgatata gcgaagatta gggctaatct cctgcacttc
    10261 tttataaccc tgacccacac catcggcgac gaccgtttta cgctgacgcg gatatttaaa
    10321 tttcacgctc ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc
    10381 gttgttggct tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag
    10441 acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt
    10501 tagcatttgg cctggcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga
    10561 cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggcgat attccatggg
    10621 atggattagc acatttatct tcgttttgcg aattacatga cagtccgaaa caagatgaaa
    10681 aagtatttaa aaaaggggga ggaagaatga gtaggacgaa ttattctgaa ccagaaagca
    10741 atcgggcagc gtcgtgctgc ccgtgtgcat acttttagtc gatggtacgc aacagttcgt
    10801 taatgccgac tttgccgcga gttttcgcgt caactttctt aacgataacc gcacagtaga
    10861 ggctgtattt gccatctttc gacggcagat tgcctgaaac aaccacagat cccgccggaa
    10921 cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat acgggtgctc tgaccaatgt
    10981 atacgcccat agagatgacg gaaccttctt cgacaatcac cccttcaacc acttcagagc
    11041 gcgcgccgat gaagcagtta tcttcaataa tggtcgggtt agcctgcagc ggctccagca
    11101 cgccgccgat gcccacacca ccggaaaggt ggacgttttt accaatctgc gcacaggaac
    11161 cgacggtggc ccaggtatca accatcgtgc cttcatcaac atatgcgccg atgttgacgt
    11221 aagacggcat cagcacggtg ttacgggcaa tgaacgcgcc ctgacgcacc gccgctggtg
    11281 gcacaacgcg gaagccttct ttctgaaaac gcgcttcgtc gtagttagcg aatttcatcg
    11341 gcactttatc gaagtagcgg ctttctgccc cttcgattac ctgattatca ttaatacgga
    11401 aagagagcag caccgctttt ttcaaccact gatgcgtcac ccactgaccg tcaatttttt
    11461 ccgctacacg cagtgcgccg gaatccagca gggcgatcac ctgatttacc gcttcgcggg
    11521 taacggtgtc tgcgttggct ggcgtgatct cggcacggcg ttcaaaagcg gtttcaataa
    11581 tgttctgtaa ctgctgcatt gttaaactct tttcatatca gtaaacacat caccctttat
    11641 cgtttggatt gagggcctct gtcaaccgct gatgcacttc ctgctgcaac tcgttattaa
    11701 gcgcacgccg gtcagcggtg gcaattatga ataaatcttc tactcgctcg ccaatggttg
    11761 taattcgggc gccatgaagc gaaattccca gatcggcaaa aattttccca actcgcgcca
    11821 gcagacctgg ttggtcgagg gcgatcagtt cgaggaacga tttgcggtcg gtatgggtcg
    11881 gcaaaaacgt tacttcggtt tcaacagtaa aatggcgcaa tttggcagat tgacgacggg
    11941 gctgcggtgg ctgccagcta ctttgtgtca gtacttgctc cagaccaaac cgaataacct
    12001 catgacgatc tgcggacagc gggctgccgt cgggttccag cacgatgaag gtatccatcg
    12061 ccataccgtc gcgagtggtg aaaatttgtg cgtcgtggac acttaaattg cggcggtcta
    12121 attcggcaca gacggcggca aacagatagg ggcggtccgg gctccagata aaaatctcgg
    12181 tgcctccacg cgtagcctgc ggactaagca ataccagcgg tttgcttaaa tcatgctgta
    12241 ataaatggcg cgcatgccag gccagttgat ttgggctatg gcggacaaaa tagttagcac
    12301 gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat gttatccatg cgcagtagtg
    12361 ccagcgcctg gagttggtga tggcgaaccc gttcgcgcat atccggcgtg ttttgcattc
    12421 cgcgtcgtag ctgcttttcg gtggcgaaat agagttcgcg caacagactt tgcttccagc
    12481 tattccacag cgtttcgttg gtggcgcaaa tgtcagccac tgtcaggcat accagatagc
    12541 gcagacgatt ttccgtttga acttcttcgg caaactgctt gatcacttcc gggtcctgaa
    12601 tatcgcggcg ctgtgctgtc accgacatca gcaagtgctg gcgaaccagc caggcgacca
    12661 gctgtgtttc gcgtgagttc agcccgtgga gttcggcaaa atgcactaca tcctgagcac
    12721 cgagaataga gtggtcgccg ccgcgtcctt tggcgatatc gtgaaacagc gcggcgatga
    12781 aaatcagctc agttgacggc aggcgtggcc agacgtccac acacaacgga tggcgctggc
    12841 gcgtttcttc actggcaaaa ctctccagtt tcagcatcac gcggatagta tgttcatcca
    12901 ccgtgtaggc gtggaacaga tcaaactgca tttgcccgac gatatgcgac cattgcggca
    12961 tatacgcgcc gagtacgcta tggcgatgca ttggcaatag cccgcgccgc accgctccgg
    13021 ggtgacgcag aatgctcaaa aacagttttc gtgcttccgg aatattacac agcggttgtt
    13081 gcagatggcg acgggcatgg cgcagatggc gcagcgtggt ggagtaaatg ccggtgatcg
    13141 cactgttgcg caccatggtg tagaacatac gcaagatggc ttccggctgg cgcataaata
    13201 gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg aaactcatcg tcgattggac
    13261 gtggtttttc gtcggcggga agggcgagga tggcttcatc gaacagttgc agcagcatct
    13321 ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc cttcatcatc cgctcgaccg
    13381 gttcgttacc ttcaccactg taattcaaac gctgggcgac gctaagctgg cgatcaaaca
    13441 gcaggcgatt atcgtaacgg ctgacgacca gatgcagggc aaagcgaata cgccacaata
    13501 tatgcagaca ttcgtttaat tccgctcgct ccgctgaggt taagaagcca aacccgacca
    13561 tttcatccag cgatgttgcg ccaaaatgac ggcgggccac ccattgcagc gtgtggatat
    13621 cgcgcaagcc gccagggcta cttttgatgt ctggctcaag gttgtagctg gtgccgtggt
    13681 aacgctgatg gcgctggttc tgttcttcaa ctttcgctgc gtagaacttg tcggaaggcc
    13741 agaatccttc gctaaaaata tgtttttgca gttcgaggaa gagcgctaca tcgccaatta
    13801 ataagcggga ttcgattaaa ttggtggcga cggttaaatc cgataatcct tccagcatgc
    13861 actcttcaag cgtgcgcacg ctatgaccga cttccagctt cacatcccag agcagcgtta
    13921 acagctcgcc cactttttgc gcctgatcat ccgggagctt tttacggctt aaaatcagta
    13981 aatcgatgtc ggaaagtgga tgcagctcgc cacgaccata accaccgacg gcaaccaatg
    14041 ccaggtcggc aatctggctg aatcccgctt caatccataa tcgttgcagg agctggtcta
    14101 tgaactcggt acgtgcctca atcaactgtt ctgcggagat cccattgtca aaggcatcac
    14161 ccagccaacg ctggaaagta tcgatatggg cttttatccc acagacggtt aattcatcac
    14221 ggggccaggc acatggattt tgcggttgac cgggcagggt ggggagagcg gtgtttgcgt
    14281 actgttctgg aagggtattc attgtgcgcc acccataaga ttaaattatc gcattaaaaa
    14341 agccggcatt cgccggcttc atcttattcg tcgtgcgaga ttatcgccgg gatggtgtca
    14401 tccttgcgta gcgtcagaat ttcgcagccg ttatcagtca ccacaatagt atgctcgtat
    14461 tgtgcagata agctgcgatc tttggttttt accgtccagc catctttcat ggtgcggatc
    14521 tcttttttac ccgcgttgac cattggctcg atggtgaagg tcatccccgg tttcagcacg
    14581 acgttggttt cacgggagtc atagtgcagc acctgcggtt cttcatggaa gccgcgacca
    14641 ataccgtgtc cacaatattc acgaacgacg gagaagcctt ctgcttcgac aaacttctga
    14701 attgccgcac cgatttcgcg cagattaatg ccaggtttta ccatgcgcag cgccaggtac
    14761 aggctttctt gcgtgatgcg gcacagacgt tcgcccatga tggtcggctt accgacgata
    14821 aacattttcg aggtatcgcc gtggaaacca tctttgatta cggtgacatc aatgttaacg
    14881 atatcgccat ctttcagcag cttggcgtcg tccgggatac cgtggcacac cacttcatta
    14941 atagaaatgc aaacggattt cggatagccg tgatagccga ggcaggcaga aaccgcgtgt
    15001 tgttcattaa caatgtaatc attacagatg cgatccagct cgccggtgct gacgcccggt
    15061 ttaacatacg gttcgatcat ctccagcact tcggcagcca gtcggccagc gacgcgcatt
    15121 ttttcgatat cttctggggt cttgattgag atagccatta attctgtcca tcagcgtcgg
    15181 tgataccgac aatatatatg taagtgccgt caatggtatc acacccgggc aaattgagaa
    15241 tcattctgaa tttcgccaaa cgtgccactg aaggttttct ataatagaaa attcaacgtc
    15301 tgatgctgta cacagcgcca acaattattg gtgtccgcga cgtatttgtg gtataaagcg
    15361 cgccggactt ccgatccatt tcgtatacac agactggacg gaagcgacaa tctcactttg
    15421 tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc ctttggggtc ggtaatatgg
    15481 gatacgtgga ggcataaccc caacttttat atagaggttt taatcatggc aactgtttcc
    15541 atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc agacccgtta ctggaacccg
    15601 aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc acatcatcaa ccttgagaaa
    15661 actgtaccga tgttcaacga agctctggct gaactgaaca agattgcttc tcgcaaaggt
    15721 aaaatccttt tcgttggtac taaacgcgct gcaagcgaag cggtgaaaga cgctgctctg
    15781 agctgcgacc agttcttcgt gaaccatcgc tggctgggcg gtatgctgac taactggaaa
    15841 accgttcgtc agtccatcaa acgtctgaaa gacctggaaa ctcagtctca ggacggtact
    15901 ttcgacaagc tgaccaagaa agaagcgctg atgcgcactc gtgagctgga gaaactggaa
    15961 aacagcctgg gcggtatcaa agacatgggc ggtctgccgg acgctctgtt tgtaatcgat
    16021 gctgaccacg aacacattgc tatcaaagaa gcaaacaacc tgggtattcc ggtatttgct
    16081 atcgttgata ccaactctga tccggacggt gttgacttcg ttatcccggg taacgacgac
    16141 gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg caaccgtacg tgaaggccgt
    16201 tctcaggatc tggcttccca ggcggaagaa agcttcgtag aagctgagta ataaggcttg
    16261 ataactcccc caaaatagtt cgagttgcag aaaggcggca agctcgagaa ttcccgggag
    16321 cttacatcag taagtgaccg ggatgagcga gcgaagacaa cgcatctgcg gcgcgaaata
    16381 tgaaggggga gagcccttat agaccaggta g
//
LOCUS       DAPOZO010000103        16355 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPOZO010000103 DAPOZO010000000
VERSION     DAPOZO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.103"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            234..1412
                     /locus_tag="ROI21_003790"
     CDS             234..1412
                     /locus_tag="ROI21_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDX5112661.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLSLGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLATPKAAHFPHLRADELPVFLRR
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWPEIDFDNALWTIPESRMKMRRKHVVP
                     LSQQATDILLQLRTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSSWIEMQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSSQ
                     "
     gene            complement(1803..1991)
                     /locus_tag="ROI21_003791"
                     /pseudo
     CDS             complement(1803..1991)
                     /locus_tag="ROI21_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2034..2415)
                     /gene="tnpB"
                     /locus_tag="ROI21_003792"
                     /pseudo
     CDS             complement(2034..2415)
                     /gene="tnpB"
                     /locus_tag="ROI21_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            2414..2557
                     /locus_tag="ROI21_003793"
     CDS             2414..2557
                     /locus_tag="ROI21_003793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112662.1"
                     /translation="MAKRPEYISQLRCPAAQSDTTGQDTTLPVRQRGQSQLSPDSPYV
                     VRR"
     gene            complement(3858..4343)
                     /locus_tag="ROI21_003794"
     CDS             complement(3858..4343)
                     /locus_tag="ROI21_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dr family adhesin structural subunit"
                     /protein_id="HDX5112663.1"
                     /translation="MKKLAIIGATSVMMMTGTAQANFTSSGTNGKVDLTITEECRVTV
                     ESKSESFLRSGLIANRHITNLGIQSTGCGTGQRVALKLGAGSYDDTNGAHMTHENGTD
                     KLLVNMGSATGDGTQDGGVYYINRDGNWNGQMVFIVRNDQQHLPTGKYTLNLEGGFWT
                     K"
     gene            complement(4545..4718)
                     /locus_tag="ROI21_003795"
     CDS             complement(4545..4718)
                     /locus_tag="ROI21_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DraP"
                     /protein_id="HDX5112664.1"
                     /translation="MGNLPVGSRGLYKGKKRSAGMNELRHPGSTQMTRTGQAHRGGEH
                     RLNTGPPDWQTVE"
     gene            complement(5097..5540)
                     /gene="draD"
                     /locus_tag="ROI21_003796"
     CDS             complement(5097..5540)
                     /gene="draD"
                     /locus_tag="ROI21_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dr fimbrial tip invasin DraD"
                     /protein_id="HDX5112665.1"
                     /translation="MNGSIRKMMRVTCGMLLMVMSGVSQAAELHLESRGGSGTQLRDG
                     AKLATGRIICREAHTGFHVWMNERQVDGRAERYVVQSKDGRHELRVRTGGDGWSPVKG
                     EGGKGVSRPGQEEQVFFDVMADGNQDIAPGEYRFSVGGACVVPQE"
     gene            complement(5556..8138)
                     /gene="afaC"
                     /locus_tag="ROI21_003797"
     CDS             complement(5556..8138)
                     /gene="afaC"
                     /locus_tag="ROI21_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153002854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfaC-I/III family usher protein"
                     /protein_id="HDX5112666.1"
                     /translation="MRDTSSGRMKTGVTGLALAVMVACVMFRAESGIARTYSFDAAML
                     KGGGKGVDLTLFEEGGQLPGIYPVDIILNGSRVDSQEMAFHAERDAEGRPYLKTCLTR
                     EMLARYGVRIEEYPALFRASGEGRGASVAEEACADLTAIPQATESYQFAAQQLVLGIP
                     QVALRPQLRGIAPEALWDDGIPAFLLNWQANAGRSEYRGYGKRVTDSYWVSLQPGINI
                     GPWRVRNLTTWNRSSGQSGKWESSYIRAERGLNGIKSRLTLGEDYTPSDIFDSVPFRG
                     AMMSSDESMVPYNLREFAPVVRGIARTQARIEVRQNGYLIQSQTVAPGAFALTDLPVT
                     GSGSDLQVTVLESDGTAQVFTVPFTTPAIALREGYLKYNVTAGQYRSSDDAVEHTSLG
                     QVTAMYGLPWGLTVYGGLQGAEHYQSAALGLGWSLGRLGAVSLDTTHSRGQQKGHDYE
                     TGDTWRIRYNKSFELTGTSFTAASYQYSSDGYHTLPDVLDTWRDDRYAYRHTENRSRR
                     TTLSLSQSLGQWGYVGLNGSRDEYRDRPHRDYFGASYSTSWNNISLSVNWSRNRNSGG
                     YYGGWSRTEDSVSMWMSVPLGRWFGGTDNDISATAQMQRSTGQDTRYEAGLNGRAFDR
                     RLYWDVREQMVPGSESHADTSRLNLTWYGTYGELTGMYSYSSTMRQLNAGMSGSMVAH
                     SEGVTFGQRTGDTVALIAAPGVSGASVGGWPGVRTDFRGYTLAGYASPYQENVLTLDP
                     TTFPEDAEVPQTDSRVVPTKGAVVRAGFRTRVGGRALVSLARQDGTPLPFGAVVTVEG
                     EAGQSAGSAGVVGDRGEVYLSGLKESGKLKAQWGENSLCHADYRLPEEKGPAGIFLTR
                     TVCM"
     gene            complement(8178..8975)
                     /gene="draB"
                     /locus_tag="ROI21_003798"
     CDS             complement(8178..8975)
                     /gene="draB"
                     /locus_tag="ROI21_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021517431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dr fimbrial biogenesis chaperone DraB"
                     /protein_id="HDX5112667.1"
                     /translation="MKMRAVAVFTGMLTGVLSVTGLLSAGAYAAGGEGNMSASATETN
                     ARVFSLHLGATRVVYNPASSGETLTVINDQDYPMLVQSEVLSEDQKSPAPFVVTPPLF
                     RLDGQQSSRLRIVRTYGEFPSDRESLQWICVKGIPPKEDDRWAEGKDGEKKADKVSLN
                     VQLSVSSCIKLFVRPPAVKGRPDDVAGKVEWQRAGNRLKGVNPTPFYINLSTLTVGGK
                     EVKEREYIAPFSSREYPLPAGASGKVQWKVITDYGGTSKQFEAELKG"
     gene            complement(9245..9550)
                     /gene="afaA"
                     /locus_tag="ROI21_003799"
     CDS             complement(9245..9550)
                     /gene="afaA"
                     /locus_tag="ROI21_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFA-III adhesin operon transcriptional regulator
                     AfaA"
                     /protein_id="HDX5112668.1"
                     /translation="MRERYLYLADTPQGILMSGQVPEYQFWLLAEISPVHSEKVINAL
                     RDYLVMGYNRMEACGRHGVSPGYFSGALKRFQRVSQTVYRLVPFYFPEAGHEVHRGE"
     gene            10468..10695
                     /gene="daaF"
                     /locus_tag="ROI21_003800"
     CDS             10468..10695
                     /gene="daaF"
                     /locus_tag="ROI21_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F1845 fimbrial adhesin operon transcriptional
                     regulator DaaF"
                     /protein_id="HDX5112669.1"
                     /translation="MKINKLTLNERKNDILSYFGEINAPCRTSEVAEHLGVSAYQARH
                     YLQCLEKEGKIKRSPVRRGASTLWEISSIPP"
     gene            complement(10958..11899)
                     /locus_tag="ROI21_003801"
     CDS             complement(10958..11899)
                     /locus_tag="ROI21_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDX5112670.1"
                     /translation="MSRKKDKNTSTPHDAAFRAFMASPEVARDFVSLHLPEEYRQLCD
                     LSTLRLEPCTFVDQDLKQYACDALFSMKTVSGEDGYVYCLVEHQSTENAYMAFRMLRY
                     AVAAMQRHVEAHKVLPLVIPVLFYHGERSPYPYSMNWLDGFSDPDIAAKIYTRAFHLV
                     DVTVMDDDEIMHHRRLGALTLLMKHSRSRDLMLQMDRLVQLIQDSLNEELAQVLFHYL
                     LNGSEHVTVTFLQTLAQRLPQHEDNIMTLAERIEQKGIAKGIERGLAQGIEQGKLEGK
                     LEGKLETACNMLNAGMDRQTVLQMTGLSNDQLNMLKH"
     gene            12031..12555
                     /locus_tag="ROI21_003802"
     CDS             12031..12555
                     /locus_tag="ROI21_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HDX5112671.1"
                     /translation="MGTLSIDRKPEGIYNRSHKTVQTTPQPGKTATEYKVKPAYLTRQ
                     NDNQKAADKTPWQNMTKRQRKNRKRINRLVELWPELFSREKPKPLKVGIFDELIQDIA
                     IRELAFGAGTLRAAVASYVQSPRYYRALMAGGARYDLKGQPCGEVTPQEQKEAETRLM
                     ALNDRRKRPRSATE"
     gene            complement(12666..13199)
                     /locus_tag="ROI21_003803"
     CDS             complement(12666..13199)
                     /locus_tag="ROI21_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112672.1"
                     /translation="MQITEALLAEPGDIRRFIQQAVDHWPRLLAVHFTLHSAEGINGQ
                     QIHAFCTFFYERITERNHTASPSSPVVLRWLREQHGGATIRCLLLLSQTSICHPRASA
                     TVDEQCSQVVDLLQQTWGEISAGGQCRVERCYQVARDDTSGQYVALKTAALSFALPVV
                     SGITKHPVQSAALVSSR"
     gene            complement(13477..13710)
                     /locus_tag="ROI21_003804"
     CDS             complement(13477..13710)
                     /locus_tag="ROI21_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDX5112673.1"
                     /translation="MTSITGHNSVLLRADDPLIDMNYITSFTGMTDKWFYRLISEGHF
                     PEPIKPGRSSRGYRSEVEQWMQQRTEESRGGVA"
     gene            13993..15170
                     /locus_tag="ROI21_003805"
     CDS             join(13993..14251,14251..15170)
                     /locus_tag="ROI21_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDX5112674.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGIAPSATPITPEQQRIRELEKQVRRLEEQNTIFKKGYRALDVRLTERF
                     TIVSRLSDSHSVVSLCSALGIHRSSYRYWRKRRDTVNSARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNACQEHTCLPNLLERQF
                     AVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAY
                     EVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTDGYVGKDEARQQISGYILNYYNSVRPHHYNGGLTPEESENRYRFYCKTVANIT
                     "
     gene            complement(15422..15832)
                     /locus_tag="ROI21_003806"
     CDS             complement(15422..15832)
                     /locus_tag="ROI21_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112675.1"
                     /translation="MNGIKTGLGITPGEHIISADAALSRKIRHCFCLSCRGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCDKFF
                     PGKKFCEACGTGIYCRAVCTKYVYRYRSDLLLDP"
     gene            complement(15829..16212)
                     /locus_tag="ROI21_003807"
     CDS             complement(15829..16212)
                     /locus_tag="ROI21_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112676.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYAYYTCHLCGSVLRYHPQH
                     DTERPWFEHTDDGLTEHGHECPYVQPEHREVQLIKRLQQFVPDALPVVRKACWYCRQC
                     RHAYYGEQYCTHCQTGGFSEEAVAG"
BASE COUNT         3914 a         4721 c         3912 g         3808 t
ORIGIN      
        1 tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat tccgagtccg ggcaccactt
       61 tcacctccag attcatctcc cgaactccac gaaactcaag tataatcaca tctttacaag
      121 caaactgaac attctgaact ccaccagact ccactgaatt ctctctcaat caagcgttca
      181 ttgggggcac ctgtgggggc ccattgtgtt cagtcagttt caggagcccc catatggcac
      241 ttacagccag acaggtcgaa acagccagac ctaaagaaaa agactataaa ctctctgacg
      301 aacgtggttt atatctgctg gttaaaacca cgggagcccg ctactggcgg cttaaatacc
      361 ggatagcagg aaaagagaaa aaactgtccc tcggcgtcta tcccgatgtt tcccttgctg
      421 aagccagaat caaacgcgac gatgcccgaa aaattatctc cgaagggggt gacccgggcg
      481 aaaagaagcg aaaggaaaaa ctcactcaga aaatctctgc caccaatacg ttccatgccc
      541 tcgctacgga atggcaccag cataaatctt tatcatggtc tgaaagttac gccagaagcg
      601 tactggaggc gctggataaa gatattttcc cgtatctggg caaacgaagc gttactgata
      661 tcctcccgct ggaaatgctg gaaattctgc gtcgcataga aaaacgtggc tcactggaaa
      721 aactgcgtaa ggtccgccag tactgtaatc agatttttcg ttatgccatc gccaccggac
      781 gagccactgt caatccggca tctgaactga ccagtacgct ggcgacgcca aaagctgcgc
      841 atttccccca cctgagagca gatgagctcc ctgtttttct ccggaggctc gctgaatatc
      901 atggcagtcc tgttacccgc atggcgacaa atctgctgct tctgacaggc ctcagaacga
      961 ttgaactacg gtccgctgaa tggccagaaa ttgattttga taatgccctg tggacaatcc
     1021 ctgaaagccg catgaaaatg cgacgtaaac atgtcgtacc actgtcacaa caggccactg
     1081 acattctgct gcagctcaga accttctccg gacaataccg gctggttttt cccgggcgtt
     1141 gtgatatcaa caagccgatg agcgaagcca gcatcaatat ggtgctcaaa cgtatcggtt
     1201 acgatggcag ggcaaccggt catggttttc gtcacaccat gagcaccatt ctgcacgaac
     1261 agggctttaa ttcttcctgg attgaaatgc agttagctca tgtggataaa aacgccatca
     1321 gaggcaccta taaccacgca cagtatctgg atggtcgccg tgaaatgatg cagtggtacg
     1381 ccgactacat tgattcactc tccagccagt aaagttaagt cagaaatact tttccatgat
     1441 actttttcag ggttaagtat tttaccctcc ggcctgcagg gccggagggg ttttgttatt
     1501 aattatattt tttcatctgc aaaattatca caaaaaacat gattgcagac aaattgacat
     1561 tttcaggctg gtgattacag aatatggcct tgcacttatt ccggcacctt cagcaaagga
     1621 aaaattgatt cagatattat ttagcatttc agtcatctga catcattata aaaaatgaaa
     1681 ttcatataaa gataagatta ttgttatcaa tgccaggcag gcactggctt cgtaagtaat
     1741 gatcgttcgg ggccaggaca gagcctgatg cgtattccgc tctattcgat cacggattgg
     1801 catcgaagtc cgatcagcga ttcgcatttt ttaccaaaac gctgctggag tgagagcttc
     1861 atctgctctt ccagaatacg catacggtcc agaaggccag cattctcagc cttgactttc
     1921 tgcaccatgg caatcgccag tgtgcgaagc tgttccgggt cacgggtggt ggtgagaagt
     1981 gaaatatcca tgcggtggat tatacaccat tgggcatgga tattcattac atttcatgtg
     2041 gttactgtga tatgctgcgc atccggaggc ggttcccggt atacccattt tgccaggtca
     2101 tgccctttca cctgtggcca gtcaacgcca cagacaagcc agtgaaagcc atccggtgtg
     2161 agatgctatg tggcatcacc tttccggggc cagcggttag catccccggt gcagtcgtcg
     2221 ggtacacagc catacgccat gtttgtccca ccggagaact tttatccggg agcgggcttt
     2281 gttggtgaag acaaaagcag ccccgtcatg ccatgctgcg ttaagttcgt tctgaacgta
     2341 ctggggcagg gtgtcgatac cggcacgcat atcgacgggt ttcacggcca gccagacgtt
     2401 atcaggtgtc agcatggcga agcgccctga atacatcagt cagttgcgct gcccggcagc
     2461 acagtcggat accaccgggc aggatacaac gttaccggtt cgtcagcggg ggcagtcgca
     2521 gttgtccccc gactctccgt atgtcgtaag acgctgaccg ggatgaaagc gggtccatgc
     2581 gccttcctgt cgccccggtt attacctttt cggccatgta gcgcatggat ttatggggga
     2641 tattgtgctg ttcgcaggac gccatccagg aaggtccact ggcccgccat gggtcaacat
     2701 gttgtctttt ttgctcaagc gtccatcgtg atctggcatc gtcattttcc ctccaggttg
     2761 cagatatgca gcaatcctaa agcagcgata tttactgtga agaggagttc gccagacata
     2821 acacaaacac ggggctatcg taaaggagcc aaattttctg tgaatatgag ttacccgtat
     2881 catgacgagt tgggcccatg acttctcatc gtttgtttgt tggtttgacc tttttggcgt
     2941 gatcgtgcgc cgtcctggcc gcctcggcga tcaggagcgg ccacttatgg tcgttgatgg
     3001 ggatgtagtg ccgtttcagt gcgggatccg tgaattccat tgaacggcca agggtctatt
     3061 gggttatagg tttattttta gctcgagcgc ccggggggat ctcgcaaggt cgtttttcct
     3121 gatgacgttg tgaattcgaa agtcgtggtg ttagcgatca tgattaggtt ttctagaatc
     3181 cttaagcgca tgcatggctc agtgataata tccgctgtgc tgttagtcgc catttttgta
     3241 gcgcaacttg tttcgaattg ccttgtcgta gcacgatcga ccagtaagtt gccgccgtta
     3301 cgaggcgtat tgcctccgtg taatcggccg taacaggcac caggggcgct ccacggcgcg
     3361 cggcttcctt ggttgggcag cctacccgga tactcgcgct ggcgccatac cattaacctg
     3421 ccgcgtgcaa taggtcggtt tcgtgatagc aggacacttt ttcggacaac tgtcggttag
     3481 gaggcggcga tgccttcccg gtagttgccc cattagcgcg ttgacatcca cgtttgcacc
     3541 gtcgcgtata gcgcgtggtc ttgccaggtt gtacattgtg gcctgcgcct atatcccggc
     3601 gtttcccagt cgttcggacg gcacgggatc ggctgtcacc gtgcagggta ttcatttttt
     3661 gttctattcc acgtagcgca gctgataatg ttggggtctg atcgcggcag gcttgccccg
     3721 ggcggctgcg gccccgtcgc gatcactgtg ctgcggctcg cattatccca gctggtgcgg
     3781 gggcgttgcg gtgctcgccc ccttgttctg gtagcgtgcg gtcaggcttc cgtgaataca
     3841 accagaccgc ggtcagatta ttttgtccag aacccgcctt cgaggttcag ggtgtacttg
     3901 ccggttggta ggtgctgttg gtcatttcgt acgatgaaca ccatctgccc gttccagttc
     3961 ccgtcccggt tgatataata tacaccgccg tcttgggtcc catcgcccgt cgcagagccc
     4021 atattcacca gaagcttgtc agtgccgttt tcgtgcgtca tgtgcgcccc gttcgtgtcg
     4081 tcgtacgagc ccgcgccaag cttgagcgcg acacgttgtc ctgtcccaca ccccgtggat
     4141 tggatcccga ggttagtgat gtgcctgttg gcgatcaggc cgcttcgcaa gaacgactcg
     4201 cttttgctct cgactgtcac gcggcattct tcggttatag tcaggtcgac cttcccgttg
     4261 gtgccgctgc tggtaaaatt ggcttgagcg gtgccggtca tcatcattac gctggttgcg
     4321 cctatgatcg ctaatttttt catatggatt acctattcag ttttgtgagg gggtaacaca
     4381 acagtacggt ctaattcatc ccagaatcag taagctgtct taccccacat taaccaccgt
     4441 tcacagatgt ctgcacccgc agcagagact gttcattgca tataagtaag tttctgactg
     4501 cagggcttgc tcataaagcc gcagacaacg ttcacggtga tgccttattc cacggtctgc
     4561 cagtccggtg gccccgtgtt cagccggtgt tcgccaccac gatgcgcctg accagtgcgc
     4621 gtcatctgtg tgcttcccgg atgacgtaat tcattcatac cggcgctgcg ttttttacct
     4681 ttatataaac cacgacttcc cactggcaga ttacccataa taaaacctcc accttatttc
     4741 tgatttaaat gattctgact gtttaaatta aatccggatg gccgatttat atttatgtaa
     4801 taaaccgcca gcacggatta actgcctgtg ttaatcagct ttgtgctttc cttctctggt
     4861 cttttatttt tcctttattc tttattcttt cctgtattcc ctgcgtgccc cctccgggtt
     4921 gcaggtatcc ctccacctct tctgcggaat acaagtcacg gtccatgcgt gaatcagtgt
     4981 ttatttcatt tcagttaatt tatccggtga aataaatact ctctgtgttt tctgctttgt
     5041 ttttgtttta tgctgtgttg tgcttttgtc tttttcttct gtttttcttc tgcgctttat
     5101 tcctgtggca ccacacaggc tccgccaacc gaaaaccggt attcaccagg agcaatgtcc
     5161 tgatttccgt ccgccatcac atcaaaaaaa acctgctcct cctgaccggg cctcgacacc
     5221 cccttcccgc cttctccctt caccggcgac cagccatctc ctcctgtcct gacacgaagc
     5281 tcatgacgac catccttact ctgcaccaca tagcgctccg ccctgccgtc cacctgacgc
     5341 tcattcatcc atacatgaaa acccgtgtgc gcctcccggc agataatccg ccccgtcgcc
     5401 agcttcgcac catcgcgcag ctgcgttcct gaacctcccc ggctctccag gtggagctca
     5461 gccgcctgcg acacaccact catgaccatc agtaacatcc cgcaggtgac acgcatcatc
     5521 ttccttatac tcccgttcat ctccggctcc tcccgtcaca tacacaccgt acgggtcaga
     5581 aatatccccg caggcccctt ctcttccgga agacggtaat ccgcatggca cagactgttc
     5641 tctccccact gcgccttcag cttaccgctt tccttcagcc cgctcaggta cacctcacca
     5701 cggtctccca ccacaccggc tgagcccgca gactgacccg cttcgccctc aactgtcacc
     5761 accgcaccaa acggcagcgg cgttccgtcc tgacgggcca gactcaccag cgcacgacca
     5821 cccacacggg tcctgaatcc ggcccggacc actgccccct tcgtcggcac cacacgactg
     5881 tccgtctgcg gcacttccgc atcctccgga aacgtcgtcg ggtccagtgt cagcacgttc
     5941 tcctgatacg gtgacgcata accggccagc gtataccccc ggaaatccgt tctcacaccc
     6001 ggccagccac ccacagacgc accactcacg cccggtgccg caatcagtgc caccgtatcc
     6061 ccggtccgct gaccaaaggt gaccccctca ctgtgggcca ccatgctgcc ggacatcccg
     6121 gcgttcagct ggcgcatcgt gctgctgtaa ctgtacatcc ccgtcagttc accatatgtc
     6181 ccgtaccacg tcaggttcag acgactggtg tcagcatggc tctcactgcc cggcaccatc
     6241 tgctcacgga catcccagta cagccggcgg tcaaatgccc gtccgttcag cccggcctca
     6301 taccgggtat cctgtcccgt ggaacgctgc atctgcgccg tggcactgat gtcgttatcc
     6361 gtccccccaa accagcgtcc cagcggcaca ctcatccaca tactgacact gtcttccgta
     6421 cgcgaccagc caccgtaata gccgccgctg ttgcggttgc gtgaccagtt aaccgacagc
     6481 gagatattgt tccaggacgt actgtatgac gcgccaaaat aatcacggtg cggtctgtca
     6541 cggtactcat cccggctgcc gttcagtccc acatagcccc actgacccag ggactgactc
     6601 agactcagcg tggtacggcg actccggttc tccgtgtgac ggtatgcgta ccggtcatca
     6661 cgccaggtgt ccagcacgtc cggcagcgta tggtaaccat ccgatgagta ctgataactc
     6721 gctgccgtaa aactcgtccc cgtcagctca aacgacttgt tataacggat acgccaggtg
     6781 tcaccggtct cataatcatg tcccttctgc tgcccccggg agtgcgtcgt gtccagcgac
     6841 accgccccca gacgccccag tgaccagcca agccccagag ccgcagactg gtaatgctcc
     6901 gctccctgaa gccccccgta caccgtcagc ccccacggca gaccgtacat ggccgtcacc
     6961 tgtcccagcg acgtgtgctc aaccgcatca tccgatgaac ggtactgacc cgccgtgacg
     7021 ttgtacttca ggtacccctc acgcagcgca atggccggcg tggtgaacgg caccgtgaaa
     7081 acctgcgccg tcccgtctga ttccagcacc gtcacctgca ggtcactgcc ggaccccgtc
     7141 accggcaggt ccgtcagggc aaatgccccc ggcgccaccg tctgactctg tatcagatac
     7201 ccgttctgac gcacctctat cctggcctgc gtgcgggcaa tgccacgtac aaccggcgca
     7261 aattcacgca ggttataagg caccatactc tcatcagaac tcatcatcgc cccccggaaa
     7321 ggcacactgt caaaaatgtc tgacggcgtg taatcctcac ccagcgtcag gcgactcttt
     7381 atcccgttca gcccccgctc agcacgtatg tatgaactct cccattttcc cgactgaccg
     7441 gatgacctgt tccaggtggt caggttccta acacgccagg gtccaatgtt gattcccggc
     7501 tgcagactga cccagtaact gtccgtgaca cgcttcccgt aaccccggta ctcactgcgc
     7561 cccgcattcg cctgccagtt cagcagaaaa gccggaatgc catcatccca taacgcctcc
     7621 ggcgcaatcc ccctcagctg cggacgcagt gccacctgag ggatacccag aaccagctgc
     7681 tgggcagcaa actgataact ctccgtggcc tgcggtatcg ccgtcaggtc agcacaggcc
     7741 tcctccgcca cggaggcacc acgaccctct ccggatgcac ggaacaacgc cggatattcc
     7801 tcaatcctga ccccgtaacg cgccagcatc tcacgggtca gacaggtctt cagataaggc
     7861 ctgccctccg cgtccctctc cgcgtgaaag gccatctcct gtgaatccac acgggaacca
     7921 ttcaggataa tgtcaaccgg ataaatgccg ggtaactgcc caccttcctc aaacagggtc
     7981 aggtccaccc ccttaccgcc acctttcagc atggccgcat caaaggagta ggtgcgcgca
     8041 ataccactct ccgcacgaaa catcacacag gccaccatca cagccagcgc cagccctgtc
     8101 acccccgttt tcatccgccc tgaagaagta tcacgcatct gtcagcccgc catttaccct
     8161 gttatcacct tatgtattca acccttcagc tctgcctcaa actgcttact ggtcccgccg
     8221 taatccgtta tcaccttcca ctgaacctta cccgatgccc ccgcaggcag cggatattca
     8281 cgggaggaaa acggcgcaat atattcacgc tccttcactt ccttaccccc caccgtcagc
     8341 gtggacaggt tgatgtaaaa cggcgtcggg ttaaccccct tcagcctgtt gccggccctc
     8401 tgccactcca ccttgccggc cacatcatcc ggtcgcccct tcaccgccgg cggacgaaca
     8461 aacagcttga tgcagctgct cactgaaagc tgtacattca gggagacttt gtcagccttc
     8521 ttctccccgt ccttcccttc cgcccacctg tcatcctcct tcggcggaat gcctttcacg
     8581 caaatccact gcagactctc acggtctgac ggaaactccc cgtacgtcct gacaatacgc
     8641 agacgactcg actgctgacc atcaagacgg aacaacggtg gtgtcaccac aaaaggcgcc
     8701 ggactcttct ggtcctcact cagcacctcc gactgcacca gcatcggata gtcctggtca
     8761 ttaatcaccg tcagcgtctc ccccgacgag gccgggttgt aaaccacccg cgtggccccc
     8821 agatgcagcg agaatactct ggcgtttgtc tccgtcgcgg atgcagacat attcccttct
     8881 cccccggcgg catatgcccc cgctgacagc aaacctgtca ctgataacac tcccgtcagc
     8941 atgccggtga acacagccac agcccgcatt ttcatactcc ctccggaaca gacagataca
     9001 cgttgtcttt tatatatatt cattcaccat ccccacccgg aactgactct gaccggactg
     9061 catgcagtca ccgcattcgt gccgggtggc gaaacggatg gtgaaactga tgttaatatt
     9121 cagtgcttaa tgcttaatgc ttaatgctta atgcttaatg cttaatgctt aatgcttaac
     9181 agaaatagct ttaattagcg taataatatc aaccgcactt attaaacccg ctcacgccct
     9241 ttcatcactc tcccctgtga acttcatgac ccgcctccgg gaaataaaaa ggcaccagcc
     9301 tgtataccgt ctgactgacc cgctgaaacc gcttcagtgc accagaaaaa tatcccggcg
     9361 acacaccatg acgcccgcag gcctccatac ggttatatcc cattaccaga taatccctca
     9421 gcgcattaat aaccttctca ctgtgtaccg gcgatatctc agccagtaac cagaactgat
     9481 attccggcac ctgaccggac atcagtatcc cctgaggggt gtcagcaaga tacagatatc
     9541 gctccctcat ctctgattca ctccatacac acgaataaca tgcagggaat gaaacctttt
     9601 cacttcagat tcaacaggat acctgtctcc ccctgcgtca acggcacaaa tactatcatg
     9661 gtgtttctta cttaaaaata tacattgcac gatcttttta tctacaaaat cacaaaatac
     9721 cagaatcata taacacaaaa aagaatacaa tcagaatata tgaccggaat attattcata
     9781 tgcagaataa aagatcgtta caatcaagtt aaatggaatg agtgactgtt attgatttat
     9841 gacaaaattt ggatacaaat caaaaaacaa gttatatttt tcatgaaaag taaaaacaaa
     9901 ataaataatg taatattaat ttgtgaaatt aagatcacag atataaaaca ctgatttata
     9961 ttttcatcgt gaatatataa gacacagaaa cacaacaggg taaataaaac acttgcagga
    10021 tatgacatat cccgatgagt cattaaaaat aataagatga acacagcaca aatcattaaa
    10081 tcttctgaat atacgggaca cagaaaggga aatccggtac atcacctgtt ttcgtattgt
    10141 atgaataccc gtggcgcgac agcggacttt attgctgcaa accgtcatgc ttcccggact
    10201 cagaggagag tcacatcagc atgagcgacc aggaccacac cggtgagtgt ttttaagatg
    10261 aaaggtatgg tggcagtcac agttgcgaga accggcatac acccgtgacg ataaacaact
    10321 gtgcagaatg acaacaggag aaatgaacag taatgagggg catcattcgc agaacgccgg
    10381 atatcataca tcatccccgg tcagctggac gcatcaccgc cagaggaact ataatcatcc
    10441 ggaggctgac catctgttaa taacggcatg aaaataaaca agttaacact gaatgaacgt
    10501 aagaatgaca ttctttctta tttcggagaa atcaatgccc cctgcagaac ctcagaagtt
    10561 gctgaacatt taggggtaag tgcttatcag gccagacatt acctgcagtg tctggagaag
    10621 gaagggaaaa ttaaacgctc accggtacga cggggagcca gtacgctgtg ggaaatcagc
    10681 agtattccgc cataacccgt caggacagat gtcgttacgg acaaaaagcg gttccggaaa
    10741 acattttacc actgtgaatg accatcatcc ctgttcagac agcggctgtt tccgtgaaaa
    10801 aaatcaactg gttgctggcg atataatgat aataccttca cggagtgggt gagggacagg
    10861 gctcacccgt ccagtgcaat aaaatatgta agaatgacac aatataaata accgcctccg
    10921 ggtgcgaatg tcccggaagc gctccggact aatattgtca gtgtttcagc atattcagct
    10981 ggtcatttga aagcccggtc atctgcagca ccgtctgacg gtccattccg gcattcagca
    11041 tattgcaggc agtttccagc ttcccttcca gcttcccttc cagtttaccc tgctcaatcc
    11101 cctgtgccag tcctcgctca atgcccttgg caataccttt ctgctcaatg cgttcagcca
    11161 gtgtcataat gttgtcctcg tgctggggca gacgctgcgc cagcgtctgc aaaaacgtca
    11221 ccgtcacatg ttcactgcca ttcagcagat agtgaaacag tacctgtgcc agttcttcat
    11281 tcagggagtc ctgtatcagc tgaaccagcc tgtccatctg taacataagg tcacggctgc
    11341 ggctgtgctt catcagcagc gtcagggccc ccagacggcg gtgatgcata atttcatcgt
    11401 catccatcac cgtcacatcc accagatgga acgctctggt gtagatttta gccgcaatat
    11461 ccgggtcaga aaagccatcc agccagttca tgctgtacgg atacggactg cgctcaccgt
    11521 ggtagaacag caccggtatc accagcggaa gtaccttatg cgcctcaacg tgtcgctgca
    11581 tcgcagccac cgcatacctg agcattctga acgccatata cgcgttttcg gtactctggt
    11641 gctccacaag gcagtagaca taaccatcct caccgctgac ggtcttcata ctgaacagtg
    11701 catcacacgc atactgcttc aggtcctggt ccacaaacgt acagggctcc agccgcagcg
    11761 tcgataaatc acacaactga cggtattcct ccggaagatg cagcgagaca aaatcccggg
    11821 ccacctccgg cgacgccata aacgccctga aggcggcatc atgcggcgtg gacgtgtttt
    11881 tatccttttt ccggctcacg gtaacctgcc tgactctccc gggttatttt tcaccggaat
    11941 tattttactg atatcatgcc ccggcacaac agcacaaaat cacatgccgg aacaggatac
    12001 cgtctccggt gagcatgagg gacaatacca gtgggaacac tgagcattga ccgtaaacca
    12061 gagggaattt acaacaggtc acataaaacc gtgcagacaa caccacagcc gggtaaaaca
    12121 gccacggaat ataaagttaa acccgcatac ctcacccggc agaatgataa tcagaaggcc
    12181 gctgataaaa caccgtggca gaatatgaca aaacgccagc gtaaaaaccg caagcgtata
    12241 aatcgtcttg tggaactctg gcctgagtta ttcagccggg aaaaaccaaa accacttaaa
    12301 gtggggatat tcgacgagct gatacaggat atcgccatca gagaactggc gtttggcgct
    12361 ggtacattac gcgcggcagt ggcgtcttat gtgcagtctc cgcgctatta tcgtgcgtta
    12421 atggcaggtg gcgcccgtta cgacctgaag gggcagccgt gcggggaggt gacgccacag
    12481 gagcagaaag aggctgaaac ccggctgatg gcgctgaatg accggcgtaa acgtccgcgt
    12541 agtgcaacag aatgattcac aactacagaa tcaggttaac aacctctgat gtaacaaaaa
    12601 agcaacagca ccatcagaag atacgttttc tggtggtacg ataatactcc ggataatgcg
    12661 cggattcacc gggaggaaac cagagcagca ctctgcaccg ggtgcttcgt gattccgctc
    12721 acaaccggca gcgcaaatga taacgcagct gtttttaacg caacgtactg gccggatgta
    12781 tcatcccggg caacctgata acacctttcc acccggcatt gtccgcctgc acttatctcc
    12841 ccccaggtct gttgcagtaa atccaccact tgcgaacatt gttcatcaac tgtggcactg
    12901 gcccgcggat ggcaaatgct cgtctggctg agcagcaaca ggcatcgtat tgttgctcct
    12961 ccatgttgtt cccgcaacca gcgtaacacc accggtgagg atggactggc cgtgtgatta
    13021 cgctcagtaa tacgttcata aaagaaagta cagaatgcat gaatctgttg cccgttgatt
    13081 ccttctgccg aatggagtgt gaagtggact gccagcagac gcggccagtg gtccaccgcc
    13141 tgctgaataa aacgccggat atctcccggc tctgcaagta aggcttcggt tatctgcacg
    13201 aaatgttcct tttcaaaccg ttgcattttg ttaagccggt gcagacacac gggaaagcac
    13261 acatcaacac cagcacagta cgatacaaac acccgctact ggtgctatgg atggaatgac
    13321 gaatcagtca ggaaagcgct acaacctgct ggctgaccgg agaatgtctg caggaaggtt
    13381 cacggtaatg tcgccagcac cagggacagg gccgggctcc acaataccag cgccggcatt
    13441 ttgcacactg ccagcgcacc ggcatcacaa cacgtttcat gcaactcccc ctcgtgattc
    13501 ctctgttcgc tgctgcatcc actgctccac ttcgcttctg tacccgcggc tgctacgacc
    13561 cggttttatg ggttcaggaa aatggccttc actgatcagc ctgtaaaacc atttatcggt
    13621 cataccggtg aagctggtga tgtagttcat gtcgatcagg ggatcgtcgg cacgcagcaa
    13681 tacgctgttg tggcctgtta ttgaggtcag aatatcctct gtgagattga tgaaggtata
    13741 cgggggaact gctgaacggt gctttcagag ctggtctgct acgggagacc agggattacc
    13801 ggcaaggtaa cgggcctccg gaacgcgcac accacgacga tcgcaggcga aatcactgaa
    13861 cacccatatc acttttgagt ctctcttcgc actccggtga acgactgtac ggcaccacat
    13921 tgtagtggca cagtaaattt ggccacctga ttaaaggtga tattctcacc tcaacacaaa
    13981 acaggtgact taatgaacaa gaaaaccaaa cgtactttca cccctgagtt caggctggaa
    14041 tgtgcacagc tgattgttga taagggctat tcgtatcgac aagccagtga ggcgatgaat
    14101 gtcggttcta ccacgcttga gagctgggta cgtcagctca ggcgagagcg ccaaggtatt
    14161 gcgccctctg caacacccat tacccctgaa cagcaacgta ttcgcgagct ggaaaagcag
    14221 gttcgccgac tggaggaaca aaatacgata ttaaaaaagg ctaccgcgct cttgatgtcc
    14281 gactcactga acggtttacg atagtttcca gactgagtga cagccactca gttgtcagcc
    14341 tctgttccgc actgggaata caccgtagca gttaccggta ctggcgaaaa cgacgcgata
    14401 cggttaattc ggctcgcgtc aggttgtgca gcgaaatacg ccgggcatgg aaccagagcc
    14461 ggggctctgc aggcgcgcgc acgctggctg aaatgctgac ccaaaacggc gtcccgatga
    14521 gccgttatcg tgccggacgg ctgatgaaat acctgaacct gagcagttgt cagcccggaa
    14581 aacatcaata caaaaatgcc tgtcaggagc atacgtgttt gccgaatctg ctcgagcgtc
    14641 agttcgctgt accggagcca gacagggtat ggtgcggaga tattacgtat atctgggcag
    14701 gaaatcgctg gtgctatctg gcggtcgtca tggatctttt tgcccgcagg gttatcggct
    14761 ggagcctgtc agcgaatgcc gatactgcac tgataagcag tgccttgcgg atggcgtatg
    14821 aggtgcgtgg tcagccgcgg gacgtcatgt tccatagcga ccagggaagt cagtatacag
    14881 gactgaaata tcaacagctt ctctggcgtt acaggataaa gcaaagtgtc agccgacggg
    14941 gaaactgctg ggataacagt cctatggaac gcttcttccg cagtctgaaa acagaatggg
    15001 tgccgacgga tggttacgta ggcaaggacg aggcccggca acaaattagc ggttatatcc
    15061 tgaattatta caatagcgtc aggcctcacc attacaacgg tgggttgacg ccggaagaat
    15121 cagagaacag ataccgtttt tactgtaaaa ccgtggccaa tattacttga ccactacaca
    15181 tcccgggggg agcggcttct ggtacggtgc tgcttcaccc ggtgcggatt caacgattca
    15241 cggtgcgctt tcgcatcccg gcacactgaa tcctccggga ttctggtaag cagaactgca
    15301 gatatccgct gtcgcgtcgt tgcacggtgt gggtgcgaaa caccggataa tatgcaggct
    15361 ggtgatacct ctgaacgtcc tgagcagcgc ggactttgtc ggatgacgtg gaaacaccac
    15421 gtcaggggtc cagcagcaaa tctgaccggt acctgtaaac atactttgta cataccgctc
    15481 tgcaataaat ccccgtacca caggcctcac agaacttctt cccgggaaaa aacttgtcgc
    15541 acatcacaca atgccactcc agcacgactg gtaatggcga taaaaacatc gccatatcct
    15601 caatgtaagg gtgggacttt tccggattca ggaccacaca gtccgccttc tgttgctcgc
    15661 tcagggcatg taaatcgtgc tcaaaccacg ccccctgagc atctgtctgc aaaatcaagc
    15721 ggccacgaca ggaaaggcag aaacaatgcc ggatctttct gctgagcgca gcgtcggcac
    15781 tgataatgtg ctcacccggc gtgatcccca gccccgtttt tataccgttc atcctgccac
    15841 tgcctcctca ctgaatcctc cggtctggca gtgtgtacag tactgctccc cataataagc
    15901 gtggcgacat tgtctgcagt accagcaggc tttacgcacc acgggtaagg catccggtac
    15961 gaattgctgc agacgtttaa tcagctgtac ttccctgtgt tccggttgta cgtaagggca
    16021 ctcatgaccg tgttccgtca gcccgtcgtc agtgtgttca aaccagggac gttccgtgtc
    16081 gtgctgcggg tggtatctga gtacactgcc gcaaaggtgg caggtgtagt aagcataagg
    16141 tgcggtctgt gcggtacggg caccggtcag acgtccgttg ccatcaagtg cgagaaaaga
    16201 ttttgcgtac atcgtcaaac tcctcagccg ccagacgaca cgcagacaac agccgtcccg
    16261 gtacgggcag cgtggtcagg gtgtgaatgg cggttttcag ttcagtctca gatgggaaaa
    16321 tcagtgtggt cgctgtgaac gcgtcatcac actga
//
LOCUS       DAPOZO010000104        15696 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPOZO010000104 DAPOZO010000000
VERSION     DAPOZO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.104"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(3..584)
                     /gene="lptE"
                     /locus_tag="ROI21_003808"
     CDS             complement(3..584)
                     /gene="lptE"
                     /locus_tag="ROI21_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDX5112677.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            complement(599..3181)
                     /gene="leuS"
                     /locus_tag="ROI21_003809"
     CDS             complement(599..3181)
                     /gene="leuS"
                     /locus_tag="ROI21_003809"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDX5112678.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            3417..3899
                     /locus_tag="ROI21_003810"
     CDS             3417..3899
                     /locus_tag="ROI21_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDX5112679.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            complement(3944..4879)
                     /gene="rihA"
                     /locus_tag="ROI21_003811"
     CDS             complement(3944..4879)
                     /gene="rihA"
                     /locus_tag="ROI21_003811"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDX5112680.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAKPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMGGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(4997..5722)
                     /gene="gltL"
                     /locus_tag="ROI21_003812"
     CDS             complement(4997..5722)
                     /gene="gltL"
                     /locus_tag="ROI21_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDX5112681.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5722..6396)
                     /gene="gltK"
                     /locus_tag="ROI21_003813"
     CDS             complement(5722..6396)
                     /gene="gltK"
                     /locus_tag="ROI21_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDX5112682.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6396..7136)
                     /gene="gltJ"
                     /locus_tag="ROI21_003814"
     CDS             complement(6396..7136)
                     /gene="gltJ"
                     /locus_tag="ROI21_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDX5112683.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7306..8214)
                     /gene="gltI"
                     /locus_tag="ROI21_003815"
     CDS             complement(7306..8214)
                     /gene="gltI"
                     /locus_tag="ROI21_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDX5112684.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            complement(8611..10149)
                     /gene="lnt"
                     /locus_tag="ROI21_003816"
     CDS             complement(8611..10149)
                     /gene="lnt"
                     /locus_tag="ROI21_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDX5112685.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDESQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSTLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(10174..11052)
                     /gene="corC"
                     /locus_tag="ROI21_003817"
     CDS             complement(10174..11052)
                     /gene="corC"
                     /locus_tag="ROI21_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDX5112686.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11142..11609)
                     /gene="ybeY"
                     /locus_tag="ROI21_003818"
     CDS             complement(11142..11609)
                     /gene="ybeY"
                     /locus_tag="ROI21_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDX5112687.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11606..12646)
                     /locus_tag="ROI21_003819"
     CDS             complement(11606..12646)
                     /locus_tag="ROI21_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDX5112688.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(12799..14223)
                     /gene="miaB"
                     /locus_tag="ROI21_003820"
     CDS             complement(12799..14223)
                     /gene="miaB"
                     /locus_tag="ROI21_003820"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDX5112689.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14369..15544
                     /gene="ubiF"
                     /locus_tag="ROI21_003821"
     CDS             14369..15544
                     /gene="ubiF"
                     /locus_tag="ROI21_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDX5112690.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         3665 a         4130 c         4114 g         3787 t
ORIGIN      
        1 catcagttac ccagcgtggt agagacgcgt gcaggcgttg ccggagtatc cgttgtggtc
       61 gacgtctgtt cttcgtcgga acgaatatcc gcagcacgga tgcttggcag cttacgaatc
      121 agctgttcgg cagcacggtc gtacatctct tttacgatca tgtcttgttc gttatctttc
      181 gctaacgcca tttgcgggtt atcgaagaac gaacggaaga ctttggcgct aatcgggtag
      241 atgtcacggc cggggatcaa cacggtcgca ttgaccgtca tgatcatctg atactctgct
      301 gtttgaccgt tacggaatac cgatgcggta tctttcgcga tgctcacttt acccaaacgc
      361 agggatggaa cgtccttacg cgtggtttct ttatcaagca actcgacacc attcagacgt
      421 aactggttac gcaccgcacg gcttaatggc ccgttcggat cgcctgagtc caggatcatg
      481 accttcatag tggaaggaac ctgcgtggta tcacgcagat gccagccaca cccggcggtg
      541 attaacaccg ccagagataa caacaatgtt gccagatatc gcacgcttcc tcccgcgctt
      601 agccaacgac cagattgagg agtttaccgg gtacgtaaat cactttacgc acggtaacgc
      661 catcaagata ttttgctacc agatgttcct ggccagcacg ttcgcgaacc tgttcttccg
      721 ttgcgtccac cggaacggtg atttttgcac ggactttacc gttaacctgc accacgacca
      781 gcgtggagtc ttccaccatc gctttttcgt cagcaaccgg ccacggtgcg ttgtcgatat
      841 cgccttcgcc tttcagttcc tgccacagcg tgaagcagat gtgcggggtg aacgggttaa
      901 gcatacggac taccgccagc agcgcttcct gcattagggc gcggtcctgc tcgccatcgg
      961 tcggtgcttt cgccagtttg ttcatcagct ccataatcgc cgcaattgcg gtgttgaagg
     1021 tctgacgacg gccgatatca tcggtcactt tagcgatcgt tttatgcaca tcgcgacgca
     1081 gcgctttctg atcttcagtc agcgcatcaa cgttcagtgc cgcaacatca cctttcgctg
     1141 tgtgctcgta aaccagtttc cagacacgtt tcaggaagcg gttagcccct tccacgccgg
     1201 attcctgcca ttcgagagtc atatcagccg gagaagcaaa catcataaac agacgaacgg
     1261 tgtccgcgcc gtaacgttca accatcacct gcgggtcgat accgttgttc ttcgacttgg
     1321 acattttgct catgccggta taaaccagtt catggcctgc cgcatctttc gctttcacga
     1381 tacggccttt ctcgtcacgt tcaacgatag catcaaccgg ggaaacccag ttacgttcgc
     1441 cgttttcgcc aacatagtag aaggcatctg ccagcaccat accctgacac agtaactgtt
     1501 tcgctggttc gtcagagttc accatgcctg catcacgcat cagtttgtgg aagaagcgga
     1561 agtagagcag gtgcataatg gcgtgttcga taccaccaat gtagatatcc accggcagcc
     1621 agtagttagc cgctttggaa tccagcatac cttctttgta ctccgggcaa gtgtagcgcg
     1681 catagtacca ggaggactcc ataaaggtgt cgaaagtgtc ggtttcacgc agcgctggca
     1741 taccgttaac ggtagttttt gcccactccg gatctgcttt aatcgggctg gtaataccgt
     1801 ccatcaccac atcttccggc aggatcaccg gcagctggtc gtccggggtc ggcattacgg
     1861 taccgtcttc cagcgtcacc atcggaatcg gcgcgcccca gtaacgttga cgggaaacac
     1921 cccagtcgcg caggcggtag ttcactttac gctcgccaac gcccatcgca gtcagtttat
     1981 cggcgatagc gttgaaggcc gcttcatggt caagaccgtt gaactcgcca gagttgaaca
     2041 gaacgccttt ttcagtcagg gcttgctgag aaagatctgg ctcagagccg tcagctgcca
     2101 ggataaccgg tttgatgttc aggccgtatt tagaggcaaa ctcgtagtcg cgctggtcgt
     2161 gacccggaac cgccataact gcgcccgtgc cgtactccat caatacgaag tttgctgccc
     2221 aaacgggaat ttcttcgccc gttaatgggt gaaccgcttt aaagccagta tcgacgcctt
     2281 ttttctccat cgttgccatt tcagcttcgg caactttggt gttacggcat tcgtcaataa
     2341 aggccgccag ttcaggatta ttttccgccg ctttctgcgc cagcggatga cccgccgcta
     2401 ccgccaggta ggtacaaccc ataaaggtgt ccgggcgggt agtgtaaacg gtcagcgtgt
     2461 tgtcatagtc gttaacgttg aaggtgattt ccacgccttc ggaacgaccg atccagttac
     2521 gctgcattgt tttaacggtg tctggccagt gatccagttt atccagatcg ttgagcagct
     2581 cgtcagcgta agcagtgatt ttgataaacc attgcgggat ctctttacgc tcaactttgg
     2641 tatcgcagcg ccagcagcag ccgtcgataa cttgttcgtt cgccagtacg gtctggtcgt
     2701 tcgggcacca gttgactgca gaagtcttct tatataccag gcctttttta tacagctcgg
     2761 tgaagaattt ctgttcccaa cggtagtatt ccggtgtaca ggttgccagc tcgcggctcc
     2821 agtcataacc aaagcccagc attttgagct ggtttttcat gtacgcgatg ttgtcgtacg
     2881 tccacggtgc cggtgcggtg ttgtttttca ccgccgcgcc ttccgcagga agaccaaacg
     2941 cgtcccagcc gatcggctgc aggacgtttt tgcccagcat acgctggtag cgggcgatca
     3001 cgtcaccaat ggtgtagtta cgtacgtggc ccatgtgtag tcgaccagaa ggatagggaa
     3061 gcatagacag gcagtaatac ttctctttgc tctcgtcttc ggttacttca aatgtgcgct
     3121 tctcatccca atgaagctgt actttggatt ctatctcttc cgggcggtat tgctcttgca
     3181 tggcagccag tggtcctgtt ttcattacgg ctacaaaagt agcgtttgag gtggtttttc
     3241 agatccgcat agcatagccc aaacgtccgc gtcaaaacag cctttcgcgc actcgacgtt
     3301 gaaatgatgc ccggattatt catacattaa tttacagagt ttgtgggcgt attagcaaag
     3361 caaggaacaa agaacgtcta ttattatagt cagttaacga cccgggagat gaaacgatga
     3421 acaaggttgc tcaatattac cgtgaactgg tcgcgtcact gagcgaacgc ctgcgcaatg
     3481 gcgaacgtga tatcgacgca ctggtggaac aggcgcgcga gcgcgtaata aaaacagggg
     3541 agttaacgcg aaccgaagtc gatgagctga cgcgagctgt cagacgtgac ctggaagagt
     3601 tcgccatgag ctatgaagag agcctgaaag aagaatctga cagcgtcttt atgcgggtga
     3661 ttaaagaaag cttgtggcag gagttggcag acatcaccga taaaacgcag cttgaatggc
     3721 gcgaagtttt ccaggacctc aatcatcacg gggtttatca cagcggagaa gtggtagggc
     3781 tgggtaatct ggtctgcgag aaatgtcatt tccatctccc gatctataca ccggaagtgc
     3841 tgacgctatg cccgaaatgt ggtcatgacc agttccagag acgcccgttc gagccgtaat
     3901 gaaatgagtg gtgccggatg gcgtgatgtc atccggcgag tgattaagcg taaaatttca
     3961 gacgatcagc cagtaaatca acaaagccct gacgatcaac atcgaccatt acagtggcat
     4021 tcggtttatt gccggtcaga taataataat caacaaccgt cataccctgg gtatatttcc
     4081 cttgtgtttc cacgccaacc cagcgctcaa cagtggtaaa caactccggt tttaacagcc
     4141 aggcgatggt acatgggtca tgcagcggtg cgccgacaaa gccccatttt tcgtctttat
     4201 gatattcgag gaagaaatcc agcagttcgg caacaatggt tgaaacaggg ttgccaatcg
     4261 cgcggaaacg ctcggtgtct tcaacgtgga tttgcgcttt atgagtaaca tccagaccgc
     4321 ccatcaccac cgggatccct gactggaaaa caatttctgc cgcttccggg tcaacgtaaa
     4381 tattaaattc agccgcaggc gtccagttac caagccccat tgcgccgccc ataatcacga
     4441 tacgagcaat tttgctatgc agttccggat ggctattgag cagcaaggca acgttagttt
     4501 gcggcccggt agaaacaatc gtgacaggtt tcgcactttc acgcagcgtt ttcgccatca
     4561 gctccaccgc cgtacagttt tgcggtgcga acgtcggttc cggtaatgcc gggccgtcga
     4621 gaccgctttc gccgtgcaca ttgtccgcga taatcaactc acgcattaac ggttttaccg
     4681 cgccgcctgc taccggaata tcggtgcgat taagcaaggt cagcatacgc agaacattgc
     4741 gtaaggtttt ttctggagtc tggtttccgg cggaagacgt aattgctttg acatcaagct
     4801 ctggtgaggc gagggcaaga actattgcga tagcgtcgtc atgacctggg tcgcaatcta
     4861 acagaattgg cagtgccatt gttgctcctt gttgtgtgct tctttgcgac aagggtaacg
     4921 ccaggatgta acagatacga ggagcgaaac gataaagcgt gagatggcgc gcacttgggt
     4981 atgcgcgcca gagtgattaa tgcaggattt tcgcgaggaa gtcttttgcg cggtccgatt
     5041 tcggatcatc gaagaaagcg tctttcggcg agtcttcgac aattttaccc tcgtccataa
     5101 agatcacccg attcgccact ttacgggcaa agcccatttc gtgggtcacc accatcatgg
     5161 tcattccttc gttcgccagt tcgaccatta cgtccagcac ttcgttgatc atctccggat
     5221 ccagcgccga tgtcggttcg tcgaacagca tggcaatagg atccatacac aatgcgcgag
     5281 cgatcgccac acgctgttgc tgaccgccgg aaagctgcgc cggaaattta ttggcatgtg
     5341 cggaaagtcc cacgcgctcc agcaatttca atgctttttc acgagcgggt gctttatcgc
     5401 gtttaagcac tttcacctgc gccagggtca ggttttcaat aatcgacaaa tgggggaaca
     5461 actcgaaatg ctggaacacc atcccgacgc gggaacgcag ctttgccaga tcggttttct
     5521 tatcgttaac cacgatgcca tcgacggtaa tttcaccttg ctgcaccggt tcgaggccgt
     5581 tgacggtttt aatcagcgtt gatttgccgg aaccagacgg gccgcaaacc accaccactt
     5641 cgcctttttt cacttcggtt gagcagtcgg tcagcacctg aaagtgacca taccattttg
     5701 aaacattttt cagggtaatc attatgctgt ccttcttttc aagtagctga ccaacaacga
     5761 cgcgctaagg ctaataacga aataaacaaa tccggcaaac aggatcatct caacctgcgt
     5821 accatcacgc tcaccaatgg ttgaggcggt acggaagaaa tcggccagag ataacacata
     5881 caccagtgag gtatcctgga acagtacgat gccctgagtg agcagcagcg gcaccatcgc
     5941 gcggaaagcc tgcggcagaa taatcagttt catcgactgc cagtgagtca tccccaacgc
     6001 cagcgcggcg ctcgactgac cacgggaaat actttgaata ccggcacgga taatctctga
     6061 atagtaggcc gcttcaaaca tcgaaaacgc caccatcgcc gaaattaaac ggatatcatt
     6121 ttttggcgat aatcccagca cgttttgcag aaaacccggc acaatcaggt aaaaccacag
     6181 caaaaccata actaaaggaa tcgagcggaa tacgttaacg taggctttgg caaaccacgc
     6241 tacgggcgca aagctggaca agcgcatcac cgccagcatg gtgccccaca aaataccaat
     6301 cacgactgcc gtgacggtga ttttcagggt gatcaccagc ccatccagca gatatggcag
     6361 ggaagggaca atggaactcc agtcaaactc gtacattatt tgccccccat gttgccgggc
     6421 aggcgaactt tacgttcaac cagcgtcatc accagcatga taaaagcgtt aatcaacaca
     6481 tacgcaagcg taatggcggt aaacgactcc caggcgtggg ctgagtaatc aagcaattta
     6541 ccggcctgcg ctgccatatc caccagaccg atggtcgagg cgatggcgga gtttttcacc
     6601 aggttcatca tctcggaggt catcggcggg acgataacgc gataagcatt aggcaacagt
     6661 acatagcggt aagcctgcgg cagcgtcaga cccatcgcca gcgcggcatt cttctgccca
     6721 cgcggcagcg actggatcgc ggcgcgtacc tgttcgcaaa cacgggcggc ggtaaacagc
     6781 cccaggcaaa gcatggatga aaggaaaaac tgaatattgg gatccagctc ggccttaaac
     6841 cacatgccga ttttctccgg caacagctcc gggatcacca gataccaggt aaagaattgc
     6901 acaatcaacg gcacgttgcg gaacagttcg acatacaacg tacccagacc ggagagaaaa
     6961 cggtttggaa cggtacgtaa aatgccgaaa aatgaaccga cgaggaaagc gataatccag
     7021 gcgcagatcg acaaagcgat cgtcacctga aaaccactcc agatccaacc aagataggtg
     7081 gtgttgccga acggggcttg ttgtaaaaaa ataccccagt tccagtctat agacataaat
     7141 ctactccaga aaaaagaggg tagcagcgtt aactgctacc ctcgaagatt gttacccagc
     7201 gtattgcggt tttcaggccc gatggggaac gaccatcagg cgtatagtct gtccgtgcta
     7261 cgtaacaatc gagagggctg gaatttccgc ccctggttct tgtaattagt tcagtgcctt
     7321 gtcattcggt tctttgaaca gtgctttcat ttcgtctgac agttcgaaat tcatgttcag
     7381 gtttttcggc ggaattgggt ttttgaacca tttatcaaac catttttccg cttcaccgga
     7441 ggtctgcacc tgagcgatag tgtcatccat cagctttttg aactgcggat cgtctttacg
     7501 caacatgcaa ccgtaggctt cctgagactg cggcttgccg acgatttccc agttgtctgg
     7561 tttcttcgct ttcgcacgtt caccagccag cagagcatca tccatcataa aggcaacggc
     7621 acgaccgctt tccagggtgc ggaaagagtc accgtgatct ttggcgctga taattcgcat
     7681 attcattttt tgctcttcat tcagtttgtt gagcaaaact tcagaggtag tgccggaagt
     7741 gacgactacg gctttgcctt tcagatcggc gaaatctttg atatcgccac cctttttggt
     7801 caacaggcgc gtaccgacca cgaaaatagt gtcagagaaa gccgcctgtt tttggcgttc
     7861 gacgttgttg gtggtagaac cacattcaaa atcgaaagtg ccgttttgca gcagtggaat
     7921 acggttttgt gaggtgatcg gaatcagttt cacctgcaag tccggtttgt tgagtttctt
     7981 tttcactgct tcaacaatgg cgttggagta atcctgcgag taacccacca ctttttgctg
     8041 attgtcgtaa taagagaaag gtactgaaga ttcacggtga ccgacgacaa tcacaccgtt
     8101 tttggcgatt ttgtccagcg tactgcccgc tgccggggcg gcgtcatctg cctgtgccag
     8161 tcctgcggaa agcgccaggg cgaggattgc tgtggcaggt ttacgtaatt gcatatccaa
     8221 ctcctttatc ttctgcgtta agaacgcatg gatacccgtt gtgagtgttt gtgttgttat
     8281 cgtctgcaac tttattgtgc agtgttgtgc ctgttaggta acatttagtt tggctaaatg
     8341 taaagatatt gctgttttat tgtttgtttt tgcgagatgc gccgcaccat tccgaagcaa
     8401 aattcttaaa atgcactctt ttggtgctac ccgtggattg ctgtggtgca accgggttgt
     8461 actgatgctg gttcaagctt tgccttgtat aacaaagcaa tagatgtgcc aaagttggat
     8521 aggggaattt tgttatccgg ataacgcact gatgccgcat ccggtgagcg tgccgaaata
     8581 cgggatgtat tccggcacga taagaaggga ttatttacgt cgttgacgca ggctcatcag
     8641 cacagcagca aaaccaaaca atgccgtcag tacccacagc ggccagttgc cggtacgtgc
     8701 gtacggtgta agtccggtag tcggcgtcac gttagtggtc aacacttcgc gggtgaactg
     8761 cgggatcatt gcctgaatct caccctgcgg gccaatcacc gccgtaatac cgttgttggt
     8821 gctacgcagc aatgggcgcg ccagctccag cgcacgcatt cgcgccatct ggaagtgttg
     8881 ccatggacca atagatttac caaaccacgc atcgttggag atagtcagca ggtagtcggt
     8941 atccgggcgg aagttatcgc gcacttgctc gccgagaatg atctcatagc aaatggccgc
     9001 agtaagctca ataccattca ccgacagcgg cggctggata tatggcccac ggctgaacga
     9061 cgacatcggc agatcaaaga acggtgctaa cggacgcaga atcgactcca gcgggacaaa
     9121 ctcgccaaac ggcaccagat ggtttttgtt atagcgatcg gctgattcgt agctgtacgg
     9181 tgcaccttta cccagcgtga tgatggtgtt gtaggtatcg tagcggttct gcttattgag
     9241 acgcgcgtca acaattccgg ttacaagcgt actcccttta tcgcgcaact caccgtccag
     9301 tgctttgagg aacggttgct gattaatttc cagatcggtt atcgccgact ccggccagat
     9361 aatcaacgat gatttgccca tcagcggtgc cgtcgcgttg taataaattt tcagcgtatt
     9421 aagaagctgg ctttcgtccc acttcagcga ttgcggaata tcgccctgaa ccatcgaaac
     9481 ctgaatggtt ttctccggtt gcggggtaaa ccactggatg taacgcagcg ggaagggaag
     9541 ggcaaacagc acgacggcca ctaccagcgg acgccagttg cgtttgacca acgccagtgc
     9601 cagcaggcca ctaaccatca tcagcaggaa gttaatggct tccacgccca ttatcggtgc
     9661 cagccctttt aacgggccat caatctggct atagccgaac tgtaaccacg ggaagccggt
     9721 cagcacccaa ccgcgcagaa actcggtcac ttgccagaga gcaggggcgg caatcgccac
     9781 gcgcagccag gtggttttcg gccatagacg cgacagcacg ccagcaaaca gtccggtata
     9841 cagcgacaaa tacgccgcca gcagcaccac caggaagatg ttaaccgggc caggcattcc
     9901 gccaaaagtc gcgatgctga catagaccca gttaataccg ctgccaaaga ggccaaatcc
     9961 ccagcaaaag ccaatagcgg cagactggag tggacggcgg ttaaaggtca gcgcctgaag
    10021 ccccatcagc gaaataatcg ccgcaggcca gacgtcgtaa ggagagaagg ccagcgttcc
    10081 gcaggcaccg aataataacg ccagcagcag gcgaatgcgc tggcgttcaa ttaatgaggc
    10141 aaaagccatg tagttatcta tccagtttcg gttttattca tccagcttcg gctggggtga
    10201 gtcatccggg attttgacat gaacctgaat aatacgccga ctgtcggcca tcgccacttt
    10261 gaactggtaa ccgtcgatgt cgatggtttc gccacgcgcc ggaagatgcc caaatgcctg
    10321 catcaccaga ccaccgatag tgtcaacctc ttcatcgcta aagtgggtgc cgaacgcttc
    10381 gttgaaatct tcgatggaag ccagtgcgcg cacggtccag gtgtggcggc taagttgacg
    10441 gaagtcgata tcatcttctt cgtcatactc gtcttcaatc tcaccaacaa tcagttccag
    10501 gatgtcttca atggtcacca gaccggaaac tccaccgaat tcgtcaatga cgatcgccat
    10561 gtggtaacgc tgagagcgaa actctttcag catccggtct acgcgcttac tttcaggaac
    10621 gacaactgcc tgacgtaaca ctttgtccat gctgaaggct tcagcatcgc tgcgcataaa
    10681 cggcagcaag tctttcgcca tcagaatccc ttcaatgtga tctttgtctt cgctaatcac
    10741 cgggaaacgt gagtgggcgg actcgatgat gacatcaagg cattcgtcca gcgtctggtt
    10801 gcgtttcagg gtaatcatct gggagcgggg gatcatgatg tcgcggacgc gttggtctgc
    10861 gatgtccatc accccttcga gcatatcgcg cgtatcttcg tcgataaggt cgttctgccc
    10921 ggaatcacgg atcagcgcca gcagttcgtc acggtttttc ggttcaccgt ggaaaagttg
    10981 gctgagtaac agggagaaaa atcccttctt gttgcttatc gtgtcactac tgtgtgaatt
    11041 gtcgtcgctc atggcgtcgt atgggttctc atgttagtta atcaaaacgc cgtcgttaat
    11101 caccaacggc ggggacgtct gccagtcaaa tgcctggcaa attattcttt ctcggcaatg
    11161 tacggatcct catagcccag agcaagcata atctctgttt cgagggcttc catttcttct
    11221 gcttcgtcat cttcgatgtg atcgtaacct aacaaatgca gactgccgtg caccaccata
    11281 tgcgcccagt gcgcctccag tggtttgcct tgctcctgag cttccttctc aaccacctga
    11341 cggcagataa ccagatcgcc cagtagcgac atttccatgc caggcggcac ttcaaacggg
    11401 aaggagagca cgttggtcgg cttatcctta ccgcgatagg tcagattcag actgtggctt
    11461 tcggcggtat cgaccacgcg aatagtcact tccgactctt cctgaaactg cgggatcacc
    11521 gcattcagcc atgtttgaaa ctggctctct tccggtaacc cggaattatc ttcacatgcc
    11581 agttgtaaat cgaggatcac ctgactcatt tttgttcctg ttcttcgcgc ttgcgttctg
    11641 ctgccagcgc cgctttacgt ttttgttctg cttcttccca ggcttcataa gcgttaacga
    11701 tacgcgccac caccgggtga cgaaccacgt cttcgctgtg gaagaagtta aagctgatct
    11761 cttcaacatc ggccagcact tcgatggcgt gacgcaagcc tgacttggta ttacgcggca
    11821 ggtcgatctg tgtgacgtcg ccggtgataa ccgcttttga gttaaaaccg atacgggtca
    11881 ggaacatctt catctgttcg atggtggtgt tctggctctc atcgagaatg ataaacgcgt
    11941 cgttcagcgt acgaccacgc atataggcca gcggcgcgac ttcaataacg ttgcgctcaa
    12001 tcagtttctc gactttctca aagcccagca tttcaaacag cgcgtcgtac agtgggcgca
    12061 gatacgggtc tactttctgg cttaaatcgc caggcaggaa gcccagtttc tcaccggctt
    12121 cgaccgccgg acgagtcagc agaatacggc gaatttcctg acgctccagg gcatcaactg
    12181 ccgcagccac tgccaggtag gttttacccg tacccgccgg gccaacgccg aaggtgatgt
    12241 catggtcgag aatattggca atgtactgcg cctggtttgg cgtgcgcggc ttgattacgc
    12301 cgcgtttggt tttgatattg accgctttgc cgtactccgg cacgctctcc gcgctttgct
    12361 ccagtacccg cgcttcttta atcgcaaggt ggatctgttc cggttcgata tcctgaatct
    12421 ggccgcgcat cggggcggta tcgacataca ggctacgcag aatgtctgcc gcagcggtga
    12481 cgcaaatcgg acggccggtc agtttaaagt ggttatcgcg gcgattgatc tcgatgccga
    12541 gacggcgttc gagctgcttg atgttgtcat caaacgggcc gcacaggctc aacagacgcg
    12601 cattgtctgc tggctccagg gtgatttcgc gagtgtctat gttcaaaccg ttcctcttat
    12661 ctgtatgccg ccggaagctg aacattcacc ggcctataag gaaattattc acgccacagg
    12721 aaaaaggcgc aagcattgcg ataaagatgg ggataaagag agaaaaaaca aggcccaccg
    12781 gaacggcggg cctgagaatt acggctgata ataacccacg ccaaggtcgt tttctttgcg
    12841 ggtacgggca atcaccgatt ccggtgtttc tgccacgcgc agacccattt catcttcagt
    12901 tcgcaccact ttaccgcgca gagagttcgg gtagacgtcg gtaatttcta catcgacgaa
    12961 tttaccgatc atatccggtg taccttcgaa gttaaccact cggttatttt cagtgcgacc
    13021 ggaaagctcc atgatgctct tacgcgatgt accttctacc agaatacgct gggtggtgcc
    13081 gagcattcgg cggctccatg ccatcgcttg ctgattaatg cgttcttgca gaatatacag
    13141 acgctgcttc ttctcttctt ccggaacatc atcaaccata tcggcggctg gtgtacctgg
    13201 acgagcggag aagataaagc tgtagctcat gtcgaaattg acgtcggcaa tcagcttcat
    13261 cgttttctcg aagtcttcgg tggtttcgcc agggaagcca acgatgaagt cagaactgat
    13321 ctgaatatcc ggacgcgccg cacgcagctt acggatgatc gctttgtact ccagcgctgt
    13381 atgggtacgt cccatcaggt tcagaatgcg atcggaaccg ctctgtaccg gcagatgcag
    13441 gaagctcacc agctccggcg tgtcgcggta cacttcgatg atatcgtcgg tgaattcgat
    13501 cggatggctg gtggtaaagc gaatacgatc gatcccgtcg atcgcagcaa ccagacgcag
    13561 cagatcggca aacgatccgg tggtgccgtc gtagttttca ccacgccagg cgttcacgtt
    13621 ctgaccgagc aggttgactt cacgcacgcc ctgagccgca agctgggcga tctcaaacag
    13681 aatatcgtcg gacggacggc ttacctcttc accacgggtg taaggcacta cgcagtaggt
    13741 gcaatattta ttgcagcctt ccatgatgga gacaaacgcg gtcggccctt cggcacgcgg
    13801 ttccggcaga cggtcaaact tctcgatttc cgggaagctg atatctacaa ccgggctgcg
    13861 gtcgccgcgc acggagttga tcatctccgg cagacggtgc agcgtttgcg gcccaaaaat
    13921 aatatcgaca tagtgggcgc gctggcgaat gtgctcgcct tcttgcgatg ccacgcagcc
    13981 gccgacgccg ataatcaggt ctggattctt ctcttttaac agtttccagc gacccaactg
    14041 atggaagact ttttcctgag ccttctcgcg gattgagcag gtgttcagca gcagcacatc
    14101 cgcttcttcc gccacgtcgg tcagttgata gccgtgggtg gcatccagca gatcggccat
    14161 cttcgatgaa tcgtactcgt tcatctgaca gccccaggtt ttaatatgga gttttttggt
    14221 catcgacttg ctcttgcgaa atagtagcca ggaatgcagg gcgcatagtg taatgctttg
    14281 ctgccgttgt gaccagtatg agcgttatca gcccttgggg gtaaaaatcc tgtaaactta
    14341 aagcatattg ctaacaggat gattgaccat gacaaatcaa ccaacggaaa ttgccattgt
    14401 cggcggagga atggtcggcg gcgcactggc gctgggactg gctcagcacg gatttgcggt
    14461 aacggtgatc gagcacgcag aacctgcacc gtttgtcgct gatagccaac cggacgtgcg
    14521 gatctcggcg atcagcgcgg cttcggtatc attgcttaaa ggattagggg tctgggatgc
    14581 agtacaggct atgcgttgcc atccttaccg cagactggaa acgtgggagt gggaaacggc
    14641 gcatgtggta tttgacgcag ccgaattaaa gttgccgttg cttggctata tggtagaaaa
    14701 cactgtcctg caacaggcat tgtggcaggc gctggaagcg catccgaaag taacgttacg
    14761 tgtgccaggc tcgctgattg cgctgcatcg ccataatgat cttcaggagc tggagctgaa
    14821 aggcggtgaa gtgattcgcg cgaagctggt gattggtgcc gacggcgcaa attcgcaggt
    14881 gcggcagatg gcgggaattg gcgttcatgc atggcagtat gcgcagtcgt gcatgttgat
    14941 tagcgtccag tgcgagaacg atcccggcga tagcacctgg cagcaattta ccccggatgg
    15001 accgcgtgcg tttctgccgt tgtttgataa ctgggcatcg ctggtgtggt atgactctcc
    15061 ggcgcgcatt cgccagttgc agaatatgaa tatggcgcag ttacaggcgg aaatcgcgaa
    15121 gcatttcccg tcgcgtctgg gttacgtgac accgcttgcc gctggcgcgt ttccgctgac
    15181 gcgtcgccat gcgttgcagt atgtgcagcc ggggcttgcg ctggtgggcg atgccgcgca
    15241 taccatccat ccgctggcgg ggcagggggt gaatcttggt tatcgtgatg tcgatgccct
    15301 gattgacgtt ctggtcaacg ctcgcagcta tggcgaagcg tgggccagtt atccgatact
    15361 caagcgttac cagatgcggc gcatggcgga taacttcatt atgcagagcg gtatggatct
    15421 gttttatgcc ggattcagca ataatctgcc accactgcgt tttgtgcgta atcttggttt
    15481 gatggcggcg gagcgtgctg gcgtgttgaa acgtcaggcg ctgaaatatg cgttagggtt
    15541 gtagccttac aacattgccg gatgcatgcc aaccgtaggt cggataagac gcgtcagcgt
    15601 cgcatccgac tttgaaggat aagacgtgtc aacatcgcat tcgacattga atgaacgcag
    15661 aaaagcaaaa agctcgccga agcgagcttt tttaat
//
LOCUS       DAPOZO010000105        15631 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPOZO010000105 DAPOZO010000000
VERSION     DAPOZO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.105"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(33..1664)
                     /locus_tag="ROI21_003822"
     CDS             complement(33..1664)
                     /locus_tag="ROI21_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDX5112691.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(1884..5567)
                     /gene="metH"
                     /locus_tag="ROI21_003823"
     CDS             complement(1884..5567)
                     /gene="metH"
                     /locus_tag="ROI21_003823"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDX5112692.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5767..6591
                     /gene="iclR"
                     /locus_tag="ROI21_003824"
     CDS             5767..6591
                     /gene="iclR"
                     /locus_tag="ROI21_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDX5112693.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            6908..9094
                     /gene="espL4"
                     /locus_tag="ROI21_003825"
     CDS             6908..9094
                     /gene="espL4"
                     /locus_tag="ROI21_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HDX5112694.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQTDKKEITIINKNENTTLTQTIAPI
                     FEEYLMEILPQRSDALDKQKLNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAA
                     KYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGL
                     VMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKI
                     RIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDE
                     ITPPLSPSTMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSINLPGLYLAINY
                     GNADIVETIFNSLSEPRYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSIL
                     NALPKLAATHHLDNEQVYKFLRAKNRSSSHVLYHVMANGDADMLKIVLDALPLLIRTC
                     HLTKEQVLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPCLAQEINISASDIVDL
                     LTAKSLARDTGLFMAMQRGHMNVIKTIFNALPTLFNTYKFDKKNMKPLLLANNSNEYP
                     GLFSAIQHKQQNIVETVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELAFELGH
                     RVIAELILNTLNKMAESYGFTDNPRYIAEKNKMETLLKKPSPHTAR"
     gene            complement(9063..10799)
                     /gene="aceK"
                     /locus_tag="ROI21_003826"
     CDS             complement(9063..10799)
                     /gene="aceK"
                     /locus_tag="ROI21_003826"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDX5112695.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFYPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIRDGHVEDVYAYRRR
                     QRFSVRYGEMAF"
     gene            complement(10848..12152)
                     /gene="aceA"
                     /locus_tag="ROI21_003827"
     CDS             complement(10848..12152)
                     /gene="aceA"
                     /locus_tag="ROI21_003827"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDX5112696.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(12182..13783)
                     /gene="aceB"
                     /locus_tag="ROI21_003828"
     CDS             complement(12182..13783)
                     /gene="aceB"
                     /locus_tag="ROI21_003828"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDX5112697.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKHILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSRGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(14052..14981)
                     /gene="metA"
                     /locus_tag="ROI21_003829"
     CDS             complement(14052..14981)
                     /gene="metA"
                     /locus_tag="ROI21_003829"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDX5112698.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            15138..15581
                     /locus_tag="ROI21_003830"
     CDS             15138..15581
                     /locus_tag="ROI21_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDX5112699.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFVDPDARGCGVGRMLVEHALSMAPEL
                     TTNVNEQNKQAVGFYKKMGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         3873 a         4030 c         3890 g         3838 t
ORIGIN      
        1 acgcgtctta tcaggccttc aggggcgcag tattaatact cgtcccgtcc ttcatcttca
       61 tcaggctgtt ccagcacact gtaagcgact gaacaaaaca gcgagttcag acgttgcata
      121 tctcccaata agcctaaatg tagcgaactg gtttcaatac tctgcacgtt ttgctgatgc
      181 aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa tgcgaaaacg atgtttgctg
      241 cggcgcaaac gacgggcgct ggtaacatcg ccagagaaga aaaccgacat tgccagtttt
      301 aaattactga gcaattgctc atagagcgca tccagttcct tcaagccatc aagcgaaaat
      361 gctcgccttg cagccagcga tttgtcggca atttcactac ccatgcgctc gacgatatcg
      421 gaggcctgtt caaggttgag cgacatttcg atgatctccg cccagcggcg cgactcttct
      481 tctgccagtt cctcttttgg cattcgcgcc agataaagtt taatcgcggt atagagcacg
      541 ttgatatcat ccgccagctt gcgcagctct ttctcctgcc gtggctcgcc gtgcatcact
      601 ttattcaacc cttccatcat ctgttccatg gcgtcgccaa tgcgcagggt ttcgcgcgcg
      661 gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga catccagatg tttgggccgt
      721 agctgggtat ccagttccgg ttcatcgcga ataatcgttt tgcaaaaccg tgccatcggg
      781 tcaacaaatg gcagcatgac caggcaacgt acaaggttgt agaagacatg gaaatagatc
      841 accagttccg cttttggcaa tgacaacttc cccattgtct ctgccagcaa atggacaaac
      901 ggcaggataa tcaggctacc caccagctta aacagcagac tacccagcgc gacacggcgg
      961 gctgcggcat tggcggcact gttgttgagc atcgccagca ggccggaacc gaggttagca
     1021 ccaatcacca gacagagcgc cacggggaag gagataatgc ctgcagcggt cagagtcgcg
     1081 gtcagcagta cagcagcaag gctggagtag ctgataatgg cgaacatcgc gccaatcagc
     1141 gcatccagca aaatgtcgcc agtcagcgag gcaaagatca cctgaacgcc gtttgcctgg
     1201 gtgatcggcg ttacggcctg cacaatcaac tccaacgcca gcaaaatcaa cccaagacca
     1261 ataccgacgc ggcccagttg cccggcgcgt gactgtttgc gtccgaggaa aaaaatcacg
     1321 ccgataaaaa taagtaatgg tgacagccag gataaatcga aggtgagaat acgcgccatt
     1381 agcgccgtcc cgacatctgc acccagcaca atcaccagag ccggtgcgag ggctaccaga
     1441 tcctgagcga cgaacgaggt caccagcatg gtggtggcat tactgctctg taccagtgcg
     1501 gtaacgccga tccccgcgca aaaggcgagc ggcttttttt cgacgctccg gctaaggaca
     1561 gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc gaacaatatg agtcccccag
     1621 accagcaggg cgacggcaga aagcaggtga agcagcgtta acacgagttc aggttctcct
     1681 tatcgttata tgttcctgaa gcaattgaat gaccaaagga ccggaaacac gctgactggc
     1741 atctttactg tgccttttga ccttcgtttt tccggctaag attattccag cctgattcca
     1801 gtataagtgt ttcatattaa taaagagaca gggcactcat tgagcgccct gtttgttgta
     1861 aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc ccagattcgg tgccagccag
     1921 cgctcaacat ctgagacgct cataccttta cggcgggcat agtcttcaac ctgatcgcgc
     1981 tggatttgcg ccaccgcgta atacttgctg tccgggtggc tgaagtacca acccgaaacc
     2041 gatgcaccag gccacatggc gaaagattct gtgagtttca tgccagtgtg tttttccact
     2101 tccagcagct cccagatggt ggctttttcc gtatgttccg ggcaggccgg atagcccggt
     2161 gccggacgga tgccctggta gttttcgcgg atcagttctt cgttgctgag gttctcgttc
     2221 ggcgcatagc cccagtagac tttacgcaca cgctcatgga gatactccgc aaaggcttcg
     2281 gctaaacggt cggcaagcgc tttcaccatg attttgttgt aatcatcgtg ctgcgcttca
     2341 aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca cggcaaatgc gccgatgtaa
     2401 tctgctttac cagaaagctt cggcgcaacg aagtcagcga gacagtagtt agcgaagccg
     2461 gttttttcgg tctgttgacg cagatggtgg ctgacgttga tcacatgggt acgcgtttcg
     2521 tcacggtaga tttcaatgtc atcgcccaca cggtttgccg ggaacaggcc caccacgcca
     2581 cgcggattca gcgttttttc ggcgcttaat ttatccagca tgtcgttggc gtctttaaac
     2641 agccgctgcg cctcaacgcc taccacttca tcttccagaa tgcgcggata cttcccggcc
     2701 agcgaccagg tcataaagaa cggtgtccag tcgatgtaat tacgcagcgt ttcgatgctg
     2761 gcttcgactt cctgcacgcc gagacggtgc gccaccggcg gcgtataagc ctgccagtca
     2821 aaagcgaagt cgttatcacg cgccgcttcc agcgtgaccg gtggtgtgcg cggtttcttg
     2881 cgcccgtgct gaatacgtac ggtttcgtac tccttgcggg tacgagcgac aaaatcatca
     2941 cgctgagtat cggaaagcag cgccgccacc acaccaacgg tgcgcgatgc gttctgtaca
     3001 tacaccgtcg ggccgctgta gttctgctca attttcaccg ccgtgtgcgc ttttgaggtg
     3061 gtcgcgccgc caatcagtaa cggaatggtg aagccctgac gctccatctc tttcgccacg
     3121 ttaaccatct cgtccagcga cggcgtgatc aatcctgaaa ggccaatcaa atcagcattc
     3181 acttctttgg cggtacggag aattttttcc gcaggcacca tcacgccgag atcgacaatt
     3241 tcgtagttgt tacattgcag caccacacca acgatatttt taccgatgtc gtggacgtcg
     3301 cccttcacgg tggcgatcac catcttgccg ttggttttgc cctgctcttt gctggcttca
     3361 ataaacggtt cgaggtaggc caccgcctgc ttcatgacgc gcgccgattt gaccacctgt
     3421 ggcaggaaca ttttcccttc gccaaacaga tcgccgacca cgttcatgcc gtccatcaac
     3481 gggccttcaa tcacttcaat cgggcgcgta gcctgctggc gggcttcttc ggtatcctgc
     3541 tctataaact cggtaatgcc tttgaccagc gagtattcca gacgtttatt cacttcccac
     3601 gagcgccact ccgcctgctg ggcgttagcg gtgtcgtcgg ttttgctgcc gcgatatttc
     3661 tcggcaagct ccagtaaacg ctcagtgcca tcgtcgcgac ggttaagaat cacatcttcc
     3721 accgcgtcgc gcagttcagc gggcaagtcg tcgtaaatcg ccagctgccc ggcgttgacg
     3781 atccccatat ccatgccatt gcgaatagcg tagtagagga acaccgcgtg aatggcttcg
     3841 cgcaccggat cgttaccacg gaatgagaaa gaaacgttag atacgccgcc ggaaatcagc
     3901 gcgtgcggca gttcgcgttt gatgtcttca cacgcgccga taaagtcctg tgcgtagttg
     3961 ttgtgctctt caatgccagt tgcgaccgcg aagatgttcg ggtcgaagat gatatcttct
     4021 ggcgggaagc caacttcttc ggtgaggatt ttgtacgccc gacggcaaat ctcgattttc
     4081 cgtgcgcggg tatcggcctg cccctgttcg tcaaaggcca ttaccaccac tgccgcaccg
     4141 taacgacgca acaatttcgc gtgatggata aaagcgtcga cgccctcttt catcgagata
     4201 gagttaacaa tgcctttgcc ctggatacac ttcagacctt tttcaatgac gtcccatttt
     4261 gaggagtcga tcataatcgg cacgcgagcg atatccggtt caccggcaat cagattgaga
     4321 aaacgcacca tcgccgcttc ggcgtcgagc atcccttcat ccatattgat atcgataatc
     4381 tgcgcaccgt tttccacctg ctgacgcgcc acatccagtg cctcgctgta tttctcttct
     4441 ttgatcaggc gcttgaactt agcggaaccg gtgacgttgg tgcgttcacc cacgttcaca
     4501 aacaggctat cgtcgccaat gttcagcggc tccaggccgg acaaacggca ggctacgggg
     4561 atttccggca gtttgcgcgg cgctaatcct tctacagcac gactcatcgc ggcaatatgt
     4621 tgtggcgtgg tgccacagca gccgccgaca atattgagaa agcccgcttg tgcccattca
     4681 cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtatt caccaaaggc gttgggcagc
     4741 ccggcgttcg ggtgcgcggt gacatagcat tccgcaatcc gtgataattc ctgcacgtac
     4801 tggcgcagtt cgtctggccc cagcgcacag ttaaggccga aagtcagcgc ttcggcgtgg
     4861 cgcaaagagt tataaaacgc ttcggtggtc tgcccggaga gcgtgcgccc ggaggcgtcg
     4921 gtgatggtgc cggagatcat aatcggcagc tcaacgccca gcgcttcaaa ctccgttttc
     4981 accgcaaaga ccgccgcttt ggcgttaagg gtgtcgaaaa cggtttcaat caggatcaga
     5041 tccgcaccac cttccaccag cgctttggtg gattcgcgat aggctgccac cagctggtca
     5101 aaagtgatgt tgcggaatgc cggatcgttg acgtccggag aaatagacgc cgtgcggttg
     5161 gtcgggccga gaacaccggc aacgtagcgc ggtttctccg gcgtgcgcgc ggtccactca
     5221 tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga tttccgccga cagggattcc
     5281 atctggtaat ccgccatcgc aatggttgtg gagttgaagg tgttggtttc gatgatatcc
     5341 gcgcccgctt caaagtaggc gttgtggata gcggcgatca cttccggttt actgagtacc
     5401 agcaggtcgt tgttgccttt gaggtcgcat ggccagtcgg caaagcgttc accacgaaaa
     5461 tcggcttcgt tcagtcgata gctctggatc atggtgccca taccgccgtc cagcaccaga
     5521 atacgttcat ttaactgcgc acgcagttgt tccactttgc tgctcacact tgctcccgac
     5581 acgctcaacc cagatttaat aaaaattcaa caaaccatac tggcataaac gcatctgtgg
     5641 taaaagcgac caccacgcaa catgagattt gttcaacatt aactcatcgg atcagttcag
     5701 caactattgc attagctaac aataaaaatg aaaatgattt ccacgataca gaaaaaggag
     5761 actgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa aacccgccgt tgccaccgca
     5821 ccagcgactg gacaggttca gtctttaacg cgtggcctga aattactgga gtggattgcc
     5881 gaatccaatg gcagtgtggc actcacggag ctggcgcaac aagccgggtt acccaattcc
     5941 acgacccacc gcctgctaac cacgatgcaa cagcagggct ttgtgcgtca ggttggcgaa
     6001 cttggacact gggcgatcgg cgcgcatgct tttatggtcg gtagcagctt tcttcagagt
     6061 cgtaatctgt tagccattgt ccatcctatt ctgcgcaatc tgatggaaga gtccggcgaa
     6121 accgtgaata tggcggtgct ggatcaaagc gatcatgaag cgattattat cgatcaggta
     6181 caatgtactc acctgatgcg tatgtccgcg cctatcggcg gtaaattacc gatgcatgct
     6241 tccggtgcgg gtaaagcctt tttagcccaa ttaagtgaag agcaggtgac gaagttactg
     6301 catcgcaaag gcttacatgc ctatacccac gcgacgctgg tgtctcctgt gcatttaaaa
     6361 gaagatctcg cccaaacgcg caaacggggt tattcatttg atgatgaaga acatgccctg
     6421 ggactgcgtt gtctcgcggc atgtattttc gatgagcacc gagaaccgtt tgccgcaatt
     6481 tcaatttccg gaccgatttc acgtattacc gatgaccgcg tgaccgagtt tggcgcgatg
     6541 gtgattaaag cggcgaagga agtgacgctg gcgtacggtg gaatgcgctg actttttctg
     6601 gcgggcagag gcaatattct gcccgtcata cccgactggc aatagaataa gggtgtctgt
     6661 taatcgcatt gacgccaaaa taacttattg tcatacactt cacacgcttt tattacctca
     6721 gcattatttt taaacatcaa accacttaat tattaacaaa atcgcaagat ctcattccac
     6781 cgaaactttt acggctgcat tatgagattg agcgatttat gatatttctt tacttgtaat
     6841 catattaaat tttttcactg ttctgatagt taaaattcaa gacatcaata aacaatgaga
     6901 tatttaaatg attactcgta ttcctcgtag ttctttctct gcaaatatta ataatacagc
     6961 ccagacaaat gaacaccaaa ccctgagtga attgttttat aaagaactcg aggataaatt
     7021 ttctggcaag gagctggcga cgcctctatt aaaaagcttc tcagagaact gtagacaaaa
     7081 tggtcgtcat atctttagta acaaggattt tgtcattaag ttttccacgt ccgtcttaca
     7141 gactgataaa aaagaaatta cgataattaa taaaaatgaa aacacgacac tcactcaaac
     7201 cattgctcca atatttgaag aatacctgat ggaaatttta cctcaacgct cagacgctct
     7261 tgataaacaa aaattaaatc taaaatcaga tagaaaagaa aaagaatttc caagaattaa
     7321 acttaatggt caatgttatt ttccggggcg accccaaaac cgtatagtat gccgacacat
     7381 tgctgcaaaa tatattaatg atatttatca gaatgttgat tacaaacccc atcaagatga
     7441 ttactcttca gctgaaaaat ttctcactca cttcaacaaa aaatgcaaaa accagacttt
     7501 ggcgttgatt tccagccgtc ctgaggggcg ttgcgttgct gcctgcggtg atttcgggct
     7561 agttatgaaa gcatattttg acaagatgga atcaaatggc atcagtgtta tggcagccat
     7621 attattggtg gataaccatg ctttgacggt ccggctaaga ataaagaaca caactgaagg
     7681 atgtacccat tacgtggtct cggtttatga tccaaatgta actaacgata aaataagaat
     7741 catgagcgaa agtaaagagg atattaaaca ctattcactg atggacttta tgaatgtaga
     7801 ttatagcctc ctgaaatggt caaatgatca tgttattaac caatctgttg caataattcc
     7861 agcacttccg aaagaacagc tattgatgtt aaaaggatct gtggatgaaa taacccctcc
     7921 attatcacct tcaacgatga atttgctcat ggcaattggt cagaatcacc aacttacgca
     7981 actgatgatt cagctccaga aaatgccaga actacataga acagaaatgt tgactgccta
     8041 taatagtatt aacctgccag gcttatattt ggctataaat tatggtaatg cggatatcgt
     8101 tgagactatt ttcaattcac tgtcagagcc tagatatgaa ggattactct cgaaaaaaaa
     8161 tctcatgcat attctggagg caaaagataa aaatggtttt tctggattat ttttagcgat
     8221 atcacgtaag gataaaaatg ttgtaacctc gattctgaac gccttaccta aactggccgc
     8281 aacacatcat ttagataatg aacaggtgta taaattcctg agagccaaaa atagatcatc
     8341 cagccatgtt ttataccatg ttatggcgaa tggtgatgcc gacatgctga aaattgtttt
     8401 ggacgcatta cctttgttaa ttcgcacatg tcatttgact aaagaacagg tactcgatct
     8461 cctgaaggca aaggattttt atggttgccc aggactatac ctggcgatgc aaaatggaca
     8521 tagcgatatc gtgaaagtta ttctcgaagc attaccctgt ctggcccagg aaattaacat
     8581 ttcagcttca gatattgtcg atcttctgac cgctaaaagt cttgcgcgcg acacgggttt
     8641 atttatggcc atgcagcgcg gacatatgaa cgttattaag actattttta acgcattacc
     8701 cactctgttt aatacgtata aattcgataa aaaaaatatg aagcccctcc tcctggcaaa
     8761 taattctaat gaatatcccg gtttgttttc agcgatacag cataaacaac aaaacattgt
     8821 agagacggtt tatcttgctt tatctgacca tgcgcgcctg tttggattta ccgctgaaga
     8881 tattatggat ttttggcaac ataaagcgcc acaaaaatac tctgcctttg agttggcttt
     8941 tgaattgggt caccgggtta ttgctgaatt aatccttaat acgttaaata agatggctga
     9001 aagctatggc tttacggata accctcgata cattgcggag aaaaataaga tggaaacttt
     9061 actcaaaaag ccatctcccc ataccgcacg ctaaacctct gcctgcgccg atacgcatat
     9121 acatcttcca catgcccgtc acggatacgg ttttgtagcg cgcgccagta gtcggcgcgg
     9181 aacaggtcgg cgtgcatctc ttcaaacagt ggcccaatac gcgggtcggc acatagccag
     9241 tggcgaaact cttccgggaa aacatcgccc ggcgagacgc tgtaccacgg ttcgctggca
     9301 agttcgtcct ccgggtagcg cggcggcggg atgtcgcgga aattcacttc cgtcatgtag
     9361 caaatttcat cgtaatcata aaacaccaca cgcccgtgac gggtgacacc gaagttttta
     9421 aacagcatgt cgccagggaa aatgttagca gcggcaagct ggcgaatagc gttaccgtat
     9481 tcttcaatgg catcgcgcaa ctgctgacct tccacttgtt ccagccagat attgagcggc
     9541 accatccgcc gctcaatata aagatggcga atcacaattt gttcgccgag atcggtgatt
     9601 ttttccgctg cttcctggag cagtaatgcc attaatgccg gggaaatatg ccgcttctcc
     9661 agcacaaagt tttcaaactc ctgggtgtcc gccattcggc ccacgcgatc atgctctttc
     9721 accagttgat agcaggcgcg aacgtgagcg gcagacatct ctttctgcgg cgcgaacttg
     9781 tctttgatca ctttgaatac ccgatcaaag cccggcagcg taaacaccag catcaccatg
     9841 ccacgaatac ccggcgcttc aatgaactgc tcattacagc cctggagata aacgagatat
     9901 tcgcggtagc tttcggtttt ggcgtgcttc tggcagccga tagccatata caattcagcg
     9961 gtggttttac ctggcagaat ttcccgtagc cactcgacca gtgctgcggg cagcggcgcg
    10021 taaaccataa aataagaacg cgcaaagcca aaaacaatgc tcgcttcggc ggtcgtcgtc
    10081 aggcaggtat caataaataa ctcgccgtcg tccgtctggt ggatcggcag caaaaatggc
    10141 aatgtgccgg aaggcgtgat cagtttgcct accagccagg cggctttatt gcggtaaaac
    10201 agttcgttcg ccacctgtaa atgactttcc gcgaggttgt ctgtccccag cgtttccgtc
    10261 agatggcgaa cgatgtaatg aatgtcacgg cttttattct gccagcgcag gcgcagcggc
    10321 aggtcgctga taacgcgcat cagcagagat tcccagccgt gatcggggta aaagtctttc
    10381 gccagcgggc ggggaatggt acgaaagcgg cgctctggct gggagctaaa gataaaaagc
    10441 cgctcgggag taagcgagcg gtggtcaaat aaccgacagt acacggagtt aaaaaagctc
    10501 tccgcaatct cgaagcgcgg gtaatccggc aacagccggg tgtaatgctc tttgacgcgc
    10561 agtaaaaatg ccgcgtccgt gctttggccg ttggtaatgc agcgcagttg ctccacgacc
    10621 agacccacgt gatgatcgta aagatggata cggtttttca tcgcctgctg gacagcgtgc
    10681 cagtcggctt gttcgaaacg ctgctgcgcc ccggaagtca cttcgaggaa tcgaccatac
    10741 tgagcgtcga aaccttgcaa aatggtttgt gcaatcagta attccaggcc acgcggcatc
    10801 gcctctcctt aattcgcccc tcgccttacg gaaaggggcg ttggttgtta gaactgcgat
    10861 tcttcggtgg agccggtcag cgcggtgact gaagacgtgc cgccctgaat aatggtcgtc
    10921 actttatcga aataacctgt acccacttcc tgctggtgag aaacgaaggt gtaaccatct
    10981 ttcgcggcgg cgaattccgg ttgctgcact ttctcaacgt agtgcttcat accttcgccc
    11041 tgggcatagg cgtttgccag gtcaaacatg ttgaaccaca tgctgtggat acctgccagg
    11101 gtgatgaact ggaacttgta gcccatatcc gacagctgct gctggaagct ggcaatagtt
    11161 ttgtcgtcga ggtttttctg ccagttgaac gacggcgagc agttataagc cagcagtttg
    11221 cccggatatt tcgcgtggat ggcttgtgca aagcgacgcg ccagttccag atccggcgtg
    11281 gaggtttcac accataccag gtcagcatat ggtgcatacg ccaggccacg gctgatcgct
    11341 tgttcaatac ctgcatgagt acggaagaag ccttcgctgg tacgctcgcc ggtaataaat
    11401 tcgctgtcat atgggtcgca atcggaggta atcagatccg ccgcatcggc atcggtacga
    11461 gcaaccagca gggttggaac gcccgtcacg tcagctgcca gacgcgccgc gaccagtttc
    11521 tgaatagctt cctgagttgg caccagcact ttgccgccca tatgaccgca tttcttcact
    11581 gatgccagct gatcttcgaa gtgaactgcc gctgcaccgg cttcaatcat cgctttcatc
    11641 agttcaaagg cattcaggac accgccaaaa ccggcttccg catcagcaac gatcggcagg
    11701 aagtaatcga catagcgcgg atcgcccggc tcaatgcccg cggaccattg gatctgatcc
    11761 gcacggcgga aagtattgtt gatccgctcc accacagctg gcaccgagtt tgccggatag
    11821 agcgactgat ccggatacat gctggccgcc aggttagcgt ccgccgctac ctgccatccc
    11881 gacagataga ctgcttcaat acccgctttc gcctgttgca gcgcctgacc gccagtcagt
    11941 gcaccgaggc tgttgatgta gccttttttc gactcgccgt gcagcagacg ccacattttc
    12001 gccgcgccca gttgcgccag ggtgcattca ggattgactg aaccgcgtaa tttcaccaca
    12061 tcttccgcgc tgtacgggcg agtaatgcct tcccaacgcg gttgagtcca ctctttctgt
    12121 aattcttcaa tttgttgtgt acgggttttc atgtgcagat gctccatagt tatgtggtgg
    12181 tttacgctaa caggcggtag cctggcaggg tcaggaaatc tattaactca tcggaagtgg
    12241 tgatctgttc catcaagcgt gcggcatcgt caaaacgccc ccgggagaaa cgttcttcgc
    12301 ccagttcgct ggcaatgact ttcatctctt cgcccagcat ctggcggaac aaggctttgg
    12361 tcaccggttt gccattgctc aacgtttttt gatgatggat ccactgccag atcgaggtac
    12421 gggaaatttc agccgtcgcc gcatcttcca tcaggccata aatcggcaca cagccgttgc
    12481 cagagatcca tgcttcgatg tactgcacag ccacgcgaat gttggcgcgc ataccttctt
    12541 cggtgcgttc accatcacaa ggtgccagca gctgatcggc agtaatcggc gcgtcttgtt
    12601 cgcgcatcac ttcaagctga tttttacggg agccgagaat gtcgttgaat accgccattg
    12661 ccgtatccgc aaggcctggg tgggcaatcc atgtgccatc gtgaccgtta ttagcttcca
    12721 gcgatttatc cgcttttact ttgttgagca cctggttatt gcgctcttca tctttgctcg
    12781 gaataaatgc cgccataccg cccatcgcaa aagcaccgcg tttatggcag gttttaatca
    12841 acaggcgtga gtaagcattc aggaagggtt tatccatcgt cactgcctgt ctatctggca
    12901 ggacgcgatc gggatagttt ttcaatgttt tgatatagct gaagatgtaa tcccaacgac
    12961 cgcagttcag accaacaata tggtcacgca gcgcgtgaag gatttcatcc atctggaaca
    13021 cggcgggcag cgtttcaatc agcaacgtcg ccttgatggt gccgcgcggc agattaaagc
    13081 gatcttctgc atagctgaag acttcgctcc accaggccgc ttcctgccag gactgggttt
    13141 tcggcagata gaaataggga ccactgccct ttgccaacag tgcctgatag ttgtggaaga
    13201 aatagagcgc aaaatcaaac aggctaccgg ggattgcctc accacgccag gtgacatgtt
    13261 tttccggcaa gtgcagaccg cgtacccgac aaatcaacac cgctggattg ggcttgagct
    13321 gataaatttt gcctgcttca ttggtatagc tgatggtgcc gttaaccgca tcgcgcaggt
    13381 taatttgccc gtcgatcact ttgttccagt ctggtgccag tgaatcttcg aaatcggcca
    13441 taaagacttt cacattggcg ttgagcgcgt tgatcaccat cttgcgctca accgggccgg
    13501 taatctctac ccgacgatct tctaagtccg caggaatccc gcgaattttc cagtcagcat
    13561 cgcgaatgga agctgtttcc gaaataaaat caggcaacgt tccgttatcg atatcctgct
    13621 gctgctgaat gcgcgctgcc agaagtttat tgcgttgtgg cgtaaaatgc gtcaccagct
    13681 cagtcagaaa ttctaccgct tcggcagtaa gaatatgctt ctcctgctcg ccatacggcc
    13741 ttgtgaaagc caattcatcg gttgttgttg cctgttcagt catcgtgcag ctcctcgtca
    13801 tggatccgaa aacttcccca ctgtgaacga aggatcgttg tgcgcttttc gttcagcaca
    13861 actaagacta ctcatttaaa atgcaaaatc aaaaacaatt tccattttaa ttaaaaatac
    13921 ttgataactt attgataaca atatgaataa aatttattca cctgctgagg tgtgtttcgg
    13981 aaataaaaaa ggcacccgaa ggtgcctgag gtaaggtgct gaatcgctta acgatcaact
    14041 atcacagaag attaatccag cgttggattc atgtgccgta gatcatatgg cgtgatctgg
    14101 tagacgtaat agttgagcca gttggtaaac agcaaattac cgtgactacg ccagctcgct
    14161 cgcggtgtat tttgcggatc attatgtgga aaatagttat acggtacatc cgggtccagt
    14221 ccggcttcca catcgcggaa aaattcctgc gccagcgttt gcgcatcata ttcgggatgg
    14281 cccgtcacaa aggcaatgcg cttatctttg ctggcaaaca gataggcatc cccttcttct
    14341 gtctccgcca gaatttccag atcggtataa tcacgaatca acgctgccgg aaagtcagca
    14401 tagcgcgaat gtggtgccag gaatgaatca tcaaagccac gcgtcagaag cgcatgagga
    14461 tggagaatat gatgctcgta aacgccagag agtttgtcgg tgcgggtttg cttaggaatg
    14521 ccgtagagga tattgagcgc ggcctggacc gcccaacaga caaacagcgt cgaggtgacg
    14581 tgatctttcg accactccag cacctgtttg atctgcggcc agtaagcgac atcattaaac
    14641 tccaccaggc ccagcggcgc gccggtaact atcagaccgt caaagttttg ttcctgaata
    14701 tcttcaaagt tacagtagaa gttattcaga tgctctgcgg gcgtgttgcg cgattcacgg
    14761 gaatcgatgc gcaacagctg aatatcgacc tgcaaaggtg agtttgaaag caggcgcaga
    14821 aactgatttt cagtttcaat cttcttcggc atcaggttta ggatcagaac cttaagtgga
    14881 cgaatttcct gaccagacgc acgagaagtt gtcatcacaa aaacgttttc ttcacgcaag
    14941 aaattgacgg cgggtagctc gtccggcaca cgaatcggca taacctgatt acctcactac
    15001 atacgcttat acgtttagac atccagatag ccgaagataa ccagaaaatt tgccaatgtc
    15061 gagcctgcat gttgaaggtg agaaagtttc aaggaaaagg ctacgttaga atataagaat
    15121 gacgaaaagg agagaaaatg gttattagta ttcgccgctc acggcatgag gaaggggagg
    15181 aactcgttgc gatttggtgt cgctctgtcg atgccactca cgatttttta tcagcagagt
    15241 atcgggccga gctggaagag ctggtgcgtt ccttcctgcc ggaagcgccg ttgtgggtcg
    15301 cggttaatga gcgggatcgg ccggttgggt ttatgttgtt aagtgggcag catatggatg
    15361 cactttttgt cgatcctgat gcgcgcggtt gtggcgtggg ccggatgctg gtggaacatg
    15421 cgctctcgat ggcaccggaa ctgacaacca atgttaatga gcaaaataag caggctgttg
    15481 ggttctataa gaagatgggt tttaaggtta cgggacgctc tgaggtggac gatttgggga
    15541 aaccgtatcc gttgctgaat ctggcgtatg tgggggaata agctgcccct gccgtgcctg
    15601 cccaggctgg agaagacgcg ccagcgtcgc a
//
LOCUS       DAPOZO010000106        15511 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPOZO010000106 DAPOZO010000000
VERSION     DAPOZO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.106"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            61..585
                     /gene="tnpA"
                     /locus_tag="ROI21_003831"
     CDS             61..585
                     /gene="tnpA"
                     /locus_tag="ROI21_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDX5112700.1"
                     /translation="MKKETDIRRGRHCVFLKHVHLVFVTKYRRQIFDHDATEKLRTYF
                     SNVCADFEAERVEMDGGPDHVHLLINYPPKLAISSLVNSLKGVSSRLLRRDRPDIAVR
                     YYDKGVLWSPGYFVSSCILTYQLDLITTISVPNRISNVISIPLIIPSTSCNFPTLELH
                     IVQTPDAKKSHVHF"
     gene            complement(742..1539)
                     /locus_tag="ROI21_003832"
     CDS             complement(742..1539)
                     /locus_tag="ROI21_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX5112701.1"
                     /translation="MLAKDKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1549..2100)
                     /gene="ybcL"
                     /locus_tag="ROI21_003833"
     CDS             complement(1549..2100)
                     /gene="ybcL"
                     /locus_tag="ROI21_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDX5112702.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(2269..2601)
                     /gene="emrE"
                     /locus_tag="ROI21_003834"
     CDS             complement(2269..2601)
                     /gene="emrE"
                     /locus_tag="ROI21_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDX5112703.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2945..3259)
                     /gene="fliE"
                     /locus_tag="ROI21_003835"
     CDS             complement(2945..3259)
                     /gene="fliE"
                     /locus_tag="ROI21_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDX5112704.1"
                     /translation="MSAIQGIEGVISQLQTTAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3474..5132
                     /gene="fliF"
                     /locus_tag="ROI21_003836"
     CDS             3474..5132
                     /gene="fliF"
                     /locus_tag="ROI21_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDX5112705.1"
                     /translation="MNAAAAQTKSLEWLNRLRANPKIPLIVTGSAAVAVMVALILWAK
                     TPDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQALAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            5125..6120
                     /gene="fliG"
                     /locus_tag="ROI21_003837"
     CDS             5125..6120
                     /gene="fliG"
                     /locus_tag="ROI21_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDX5112706.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            6113..6799
                     /gene="fliH"
                     /locus_tag="ROI21_003838"
     CDS             6113..6799
                     /gene="fliH"
                     /locus_tag="ROI21_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDX5112707.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6799..8172
                     /gene="fliI"
                     /locus_tag="ROI21_003839"
     CDS             6799..8172
                     /gene="fliI"
                     /locus_tag="ROI21_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDX5112708.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            8191..8634
                     /gene="fliJ"
                     /locus_tag="ROI21_003840"
     CDS             8191..8634
                     /gene="fliJ"
                     /locus_tag="ROI21_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDX5112709.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8631..9758
                     /gene="fliK"
                     /locus_tag="ROI21_003841"
     CDS             8631..9758
                     /gene="fliK"
                     /locus_tag="ROI21_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDX5112710.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALTGEATTD
                     KATPQLLVATDKPTAKGEPLVSDILSDAQQADLLIPVDETPPVINDEQSTLTPLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDGPGTPAQPLTALVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLQPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9863..10327
                     /gene="fliL"
                     /locus_tag="ROI21_003842"
     CDS             9863..10327
                     /gene="fliL"
                     /locus_tag="ROI21_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDX5112711.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            10332..11336
                     /gene="fliM"
                     /locus_tag="ROI21_003843"
     CDS             10332..11336
                     /gene="fliM"
                     /locus_tag="ROI21_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDX5112712.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11333..11746
                     /gene="fliN"
                     /locus_tag="ROI21_003844"
     CDS             11333..11746
                     /gene="fliN"
                     /locus_tag="ROI21_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDX5112713.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11749..12114
                     /gene="fliO"
                     /locus_tag="ROI21_003845"
     CDS             11749..12114
                     /gene="fliO"
                     /locus_tag="ROI21_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDX5112714.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            12114..12851
                     /gene="fliP"
                     /locus_tag="ROI21_003846"
     CDS             12114..12851
                     /gene="fliP"
                     /locus_tag="ROI21_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDX5112715.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12861..13130
                     /gene="fliQ"
                     /locus_tag="ROI21_003847"
     CDS             12861..13130
                     /gene="fliQ"
                     /locus_tag="ROI21_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDX5112716.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            13139..13924
                     /gene="fliR"
                     /locus_tag="ROI21_003848"
     CDS             13139..13924
                     /gene="fliR"
                     /locus_tag="ROI21_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDX5112717.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            14214..14837
                     /gene="rcsA"
                     /locus_tag="ROI21_003849"
     CDS             14214..14837
                     /gene="rcsA"
                     /locus_tag="ROI21_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDX5112718.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKFIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14881..15069)
                     /gene="dsrB"
                     /locus_tag="ROI21_003850"
     CDS             complement(14881..15069)
                     /gene="dsrB"
                     /locus_tag="ROI21_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDX5112719.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            15232..15459
                     /gene="yodD"
                     /locus_tag="ROI21_003851"
     CDS             15232..15459
                     /gene="yodD"
                     /locus_tag="ROI21_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDX5112720.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
BASE COUNT         3924 a         3913 c         3938 g         3736 t
ORIGIN      
        1 ggtcttaacc ggaatttgaa tgcctgtagt cgtttcatgg gccaaatcat actttggcct
       61 atgaaaaaag aaaccgatat ccgacgcgga aggcattgtg ttttcctgaa gcatgttcac
      121 ctagtcttcg ttactaaata ccgacgtcag atttttgacc atgacgcgac agaaaaacta
      181 cgcacttact tttcaaatgt atgtgctgat tttgaagctg aacgggttga aatggatggc
      241 ggaccagatc acgtccattt gttaattaac tatcctccca aactggcgat atccagtctg
      301 gtaaacagcc tcaaaggcgt atcgagtagg ttactgcgac gagatcgacc agatattgca
      361 gtcaggtatt acgacaaagg cgttttgtgg agtcctggct attttgtcag tagctgcatc
      421 ttaacgtatc aactagattt aataacaacg atatccgtac ctaaccggat atctaatgtc
      481 ataagcatcc ctttaattat cccctcaacc tcttgtaact ttcccactct tgagctccac
      541 atagtccaaa cgccggatgc aaaaaaatca cacgtgcatt tctgaaattg tgatggagga
      601 agatagtatc aattatatgt gcttagaaac gtccccgatt agcgcagaag tcaccggatt
      661 gttcagactc aaataggttg agtatgccgt atttattttc caaaataaaa atcgatgctg
      721 ttgctccttc ataaaatact tttactctct ggacgctttt cttagctctc ttggtgttat
      781 cttaaaacga ttatggaacc tttcagtaaa acgagaagga cacttgtaac cattttccct
      841 ggcaatctcg ctgatgggtt ttactgtcgt ttgtatagca gacaacgcat tatttaacct
      901 cacgtcgtcc aatatacttt gaaaacttac tccctcgctt gccagacggc gatgcaatgt
      961 ggaaacagaa atgtagagat atctcgctac cttgtttgct gtccattttg ttccaggttc
     1021 ggatagcatt aggttataac aacgacttat taatgattgt ttactatctg ataaaagtaa
     1081 acaattaaca tgatgtgctc ctaatgaaag tagaacgccc attgctaagt gttcctgaat
     1141 ttgcgttgag aagcctcggg aaatagatgt ttttagttgc tcccaacaat atattaactc
     1201 ggggttctga ggtaagcaga aacctgtcgt gttacgaatt tgatcagtta tcgcataaaa
     1261 tttttgaaac ttttcaatta gatcaatggg ataataaagc atttctgcaa gataaagacc
     1321 tgcttctgga taattcgcaa tataaaattc atatccacag ggaaataata ttatctgatt
     1381 attatcaaca gttagactat gcgtctccca attgataact ttttttccct gacggatacg
     1441 acataaagct ggcataagag gcttaaccct gtgaatctca tgatgtttat gcatccgtat
     1501 ttcttcaatc tttaggttag tcttatcttt tgccagcata ctcttaccct actttatctc
     1561 ataaactggt gttatctcag cggttgcaat tttattggca ttaagcatat aaccaactaa
     1621 cgctccgctg gaattagaat ccacaggaat tttatcagtt tttagagccc atactttaaa
     1681 ctggtaatga tggggtttat ctcctttggg aggacatgct ccaccaaatc ctgcataacc
     1741 aaaatcattt cggccttgaa cagcaccagt cggaagtttt gtaccatcac gtcttcctgc
     1801 atcagcgggc aaatatgtta cagttgctgg aatattagca acagtccagt gccaccatcc
     1861 actgcctgta ggagcatctg gatcatacac agttacggca aagcttttgg tgccttcagg
     1921 agcaccagac caggttaatg agggcgatgt attaccacct tcacacccaa atccagaaaa
     1981 tacatgagac gttgtaagtt gctctcctgt ttttatttca ttactagtga cctgaaatgc
     2041 tgcggcctgc gcagaaaatg ttatgaatgc caatacactt gaaacgataa gttttttcat
     2101 aaaaacctca ttgttgtgac ctatcgttat tttatttgat gtgccttgat ctcattatgc
     2161 ataaaggcgc aatgatcgcg caaatgcaat cataattata aaggtgcccc gaacattagc
     2221 cataatatgc acaaatcaga ttcctgttat ctcaaggata attatttttt aatgcggtgc
     2281 acttcgtgac aacaagttaa tgactaacac accggcacaa atcaacatca tgcctatgat
     2341 agctggcagg tccagtcgtt gaccaaaaaa tccccaggaa agcaaactaa tcagaacgat
     2401 accgacgcct gaccagatag cataagcaat ccctgtaggt atataagcca gcgtctgagc
     2461 taataaccag aatgatgcac aatagcaaat aattgtgcca acagagggcc ataaccgtgt
     2521 aaacccctct gaaaacttca ttaaggttgt accaatgacc tctgcaagta ttgcgccacc
     2581 aagataaata taagggttca tagcatattc tttcctgttc atactggaga gaattgtact
     2641 acagtttgaa cacaactcac cgttttcatc ctggtgtatg tgtatccagg gggctctggc
     2701 gtggcaagag ataaaggcag gaacaataat gatcagaagg aaccccaggc taacgaatga
     2761 gttcactctt cctgggagca aagcgaagac aaaacatgtt atcgcgttta cggatcgtgg
     2821 atggcagaaa actttcagga tcaggtatcc attttaaacc agaaattgag tgagtttgcc
     2881 ccatccatgc cccacgctgt gggaccggat ataataaaca agcatagtca acaggttaca
     2941 aaacctacac ctgcatggac atcacttcct gatacgccgc caccagctta ttacgcacct
     3001 gaatccccat ttgcatagaa actgaggctt tttgcatatc ggtcatcaca tcgtttagcg
     3061 ccacgccggg ctcaccgagg gtgaattttt ctgcctgcgt gcgggcggct gtttgtgtat
     3121 cgcttatgcg atcgagcgcg gcgtgcagct gcccggcaaa actaattgtc ggttgcggca
     3181 gtgattcctg cgcacgcgca ctcatcgccg tggtctgtaa ctggctgata accccttcaa
     3241 tcccctgtat cgctgacatt ttcatctccc ggataatttc tggtagcaaa gcctaccagt
     3301 aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac gggtttattg gcggatagaa
     3361 aaaaacgaaa gcacaaataa tgggagcgtc aatttttcga gtttgctgac ccgggagtga
     3421 gtcttgttcc actttgccaa taacgccgtc cataatcagc cacgaggtgc gcgatgaatg
     3481 cggctgcagc ccagacaaaa tctcttgagt ggcttaatcg cctgcgtgcg aatccgaaaa
     3541 ttccattgat tgttaccggt tccgcggcag tggcggtcat ggtcgcactg atcctgtggg
     3601 cgaaaacccc cgactaccgt acattattca gcaatctttc cgatcaggat ggtggcgcaa
     3661 ttgtcagcca actgacgcaa atgaatattc cttaccgctt cagcgaagcc agcggcgcta
     3721 ttgaggttcc ggcagataaa gttcacgaac tgcgcctgcg cctggcgcaa caaggtttgc
     3781 ccaaaggcgg cgcggtcggt ttcgaactgc tcgatcagga aaagtttggt atcagccagt
     3841 tcagcgaaca ggtgaattat cagcgggcgc tggaaggcga gctttctcgt accatcgaaa
     3901 ctataggccc ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg tctttattcg
     3961 tccgtgaaca aaaatcccct tctgcatcgg tgacggtaaa tctgttaccc ggccgcgcac
     4021 tcgatgaagg gcaaattagc gctattgtac atctggtttc cagcgcggtt gctggtctgc
     4081 cgccaggaaa cgtcacgctg gtggatcagg gcgggcatct gttaacccag tccaatacca
     4141 gcgggcgcga tcttaatgac gctcagttga aatatgccag cgatgtcgaa ggccgtattc
     4201 agcggcgtat tgaagcgatc ctgtcgccta ttgttggtaa cggtaatatt cacgcccagg
     4261 tcacggcgca actggacttc gccagtaaag aacaaacgga agaacagtat cgccctaacg
     4321 gtgatgaatc tcatgcggcg cttcgttcac gccagcttaa tgagagcgag caaagcggtt
     4381 ccggttatcc gggcggcgta ccgggggcgt tgtcgaatca accggcacct gcgaataacg
     4441 caccaatcag cacgcctccg gcaaatcaaa ataaccgcca gcagcaggcg agcaccacca
     4501 gcaatagtgg gccgcgtagc acacagcgga atgaaaccag taactacgaa gtcgatcgca
     4561 ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg tctgtcagtc gcggttgtgg
     4621 tgaattacaa aaccttgcca gacggtaaac cattgcctct cagcaacgaa cagatgaagc
     4681 aaattgaagc tctgacccgc gaggcgatgg gcttttctga aaaacgcggt gattcgctca
     4741 atgttgttaa ctcgccgttc aatagcagtg acgaaagcgg cggagcactg ccgttctggc
     4801 aacagcaagc gtttatcgat cagttgctgg ctgccggtcg ctggttgctg gtactgctgg
     4861 tggcgtggtt gctgtggcgg aaagcggtac gcccgcagct aacacgtcgc gccgaggcgg
     4921 tgaagactgt gcagcaacag gcgctggccc gcgaggaagt ggaagatgcg gtggaagtcc
     4981 gtctgagcaa agacgaacaa ctccaacaac ggcgcgctaa ccaacgactg ggggcagaag
     5041 tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg gcgctggtca
     5101 ttcgccagtg gataaataac gatcatgagt aacctgacag gcaccgataa aagcgtcatc
     5161 ctgctgatga ccattggcga agaccgggcg gcagaggtgt tcaagcacct ctcccagcgc
     5221 gaagtgcaaa cccttagcgc tgcaatggcg aacgtcacgc agatctccaa caagcagcta
     5281 accgatgtgc tggcggagtt tgagcaagaa gctgaacagt ttgccgcact gaatatcaac
     5341 gccaacgatt atctgcgttc ggtattggtc aaagctctgg gtgaagaacg tgccgccagc
     5401 ctgctggaag atattctcga aacccgcgat accgccagcg gtattgaaac gctcaacttt
     5461 atggagccgc agagcgccgc cgatctgatt cgcgatgagc atccgcaaat tatcgccacc
     5521 attctggtgc atctgaagcg cgcccaggcc gccgatattc tggcgttgtt cgatgaacgt
     5581 ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc agccgcgctg
     5641 gcggaactga ccgaagtact gaatggcttg ctcgacggtc agaatctcaa gcgcagcaaa
     5701 atgggcggcg tgagaacggc agccgaaatt atcaacctga tgaaaacgca gcaggaagaa
     5761 gccgttatta ccgccgtacg tgaattcgac ggcgagctgg cgcagaaaat catcgacgag
     5821 atgttcctat tcgagaatct ggtggatgtc gatgatcgca gcattcagcg tctgttgcag
     5881 gaagtggatt ccgaatcgct gctgatcgcg ctgaaagggg ccgagcagcc actgcgcgag
     5941 aaattcctgc gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga tctcgccaac
     6001 cgtggtccgg tgcgtctgtc gcaggtcgaa aacgaacaga aagcgattct gctgattgtg
     6061 cgccgtcttg ccgaaactgg cgagatggtg attggcagcg gcgaggatac ctatgtctga
     6121 taatctgccg tggaaaacct ggacgccgga cgatctcgcg ccaccaccgg cagagtttgt
     6181 gccaatggtc gagtcggaag aaaccattat tgaagaggcc gaacccagcc ttgagcagca
     6241 actggcgcaa ctgcaaatgc aggcccacga gcagggttat caggcaggga ttgccgaagg
     6301 tcgccagcaa ggtcatgaac agggctatca ggaaggactg gcccaggggc tggaacaagg
     6361 tctggcagag gcgaagtctc aacaagcgcc aattcatgcc cggatgcagc aactggtcag
     6421 cgaatttcaa actacccttg atgcacttga tagtgtgata gcgtcgcgcc tgatgcagat
     6481 ggcgctggag gcggcacgtc aggtgatcgg tcagacgcca acagtggata actcggcact
     6541 gatcaaacag atccaacagt tgttgcagca agaaccgtta ttcagcggta aaccacagct
     6601 gcgcgtacac ccggatgatc tgcaacgtgt tgatgatatg ctcggcgcaa ctttaagttt
     6661 gcatggctgg cgcttgcggg gcgatcccac cctccatcct ggcggttgca aagtctccgc
     6721 cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg caagaactct gccgtctggc
     6781 agcaccagga gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac gctggataac
     6841 tttgaagcca aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt aacccgcgct
     6901 accgggctgg tgctggaagc caccggatta caattgccgc tcggcgcaac ctgtgtcatt
     6961 gagcgccaga acggcagcga aacgcacgaa gtagaaagcg aagtcgtcgg ctttaacggt
     7021 caacggctgt ttttaatgcc gctggaggaa gtggaaggtg tcctgcccgg cgcgcgtgta
     7081 tatgccaaaa acatttcggc agaagggctg caaagcggca agcagttgcc gctcggtccg
     7141 gcgttattag gtcgtgttct ggacggcagc ggtaaaccgc tcgatggcct cccctccccc
     7201 gatacgacgg aaaccggtgc gctgattacc ccgccattta acccattgca acgtacaccg
     7261 attgaacatg tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac cgtcgggcgc
     7321 gggcagcgta tggggctgtt tgccgggtcc ggcgttggta aaagtgtgct gctggggatg
     7381 atggcccgtt acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga acgagggcgc
     7441 gaagtaaaag attttattga gaatattctc ggtgccgaag gacgtgcacg ctccgtggtg
     7501 attgccgctc cggcggatgt ttctccgctc ctgcgaatgc agggtgccgc ctatgccacg
     7561 cgcattgccg aagattttcg cgatcgtggt cagcatgtgt tgctgattat ggactccctc
     7621 acccgctacg cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc acccgccacc
     7681 aaaggttatc caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg tgccggaaat
     7741 ggcattagcg gcggcggctc gattaccgcg ttttataccg tgctcaccga gggcgatgac
     7801 cagcaggacc ccattgccga ctccgcgcgg gccatcctcg acggtcacat tgtgctgtct
     7861 cgccgactgg cggaagccgg gcactatccg gctatcgata ttgaagcgtc gatcagccgc
     7921 gcaatgacgg cgttgatcag cgagcaacat tatgcgcgag tgcgcacctt caaacagctg
     7981 ttgtcgagtt ttcagcgtaa ccgcgatctg gttagcgtcg gtgcgtatgc caaaggcagc
     8041 gatccgatgc tcgataaagc catcgccctg tggccgcagc tggagggcta tttacaacaa
     8101 ggcatttttg aacgcgcgga ctgggaagcg tctctccagg ggctggagcg tattttcccg
     8161 acagtgtcat aacccaggag ataacggcag atggcagaac atggtgcgct ggcgaccctg
     8221 aaagatctgg cagaaaaaga ggtagaggat gccgcgcgcc tgctgggtga aatgcgtcgc
     8281 ggatgtcagc aggcggaaga acagctcaaa atgctgatcg attatcagaa tgaatatcgc
     8341 aataacctca atagcgatat gagtgccggg atgaccagca accgctggat caactatcag
     8401 cagtttatcc agacgctgga aaaagccatt actcagcatc gccagcaact taatcagtgg
     8461 acgcagaaag ttgacattgc cctgaacagc tggcgagaaa agaaacaacg tttgcaggcc
     8521 tggcagacac tgcaggaacg gcaatccacg gcggcactgc ttgcagaaaa ccgcctcgat
     8581 cagaaaaaga tggatgagtt cgcccagcgc gccgccatga ggaaacctga atgattcgca
     8641 tagcgccctt gattaccgct gacgttgata acgccacatt gcctggcggc aaagccagcg
     8701 atgctgcaca agattttctc gcgttgttga gcgaagcatt aacaggtgag gcaacaaccg
     8761 acaaagcgac tccccagttg ctggtggcaa cagataagcc cacggcaaaa ggcgagccgc
     8821 tggtcagcga tattctttca gacgcgcaac aagcagattt actgatccct gtggatgaaa
     8881 caccgcctgt catcaacgac gaacaatcca cattaacacc gttaaccacc gctcagacga
     8941 taacgttggc tgcggcggct gacaacaata cggcaaaaga cgaaaaagcg gatgatctga
     9001 atgaagacgt caccgcaagc ctgagtgccc tttttgcgat gttgccgggt tttgacaata
     9061 cgcccaaagt gactgatgca ccgtcaaccg tgttaccgac agagaaacca acgcttttca
     9121 caaaactgac ttctgcgcaa ctcactacag cacagcctga tgacggcccc ggcacgccag
     9181 ctcagccatt aacagcgctg gtagcagaag cccagagtaa agcggaagtc atcagcacac
     9241 cttcaccggt gaccgctgcc gccagcccgc taatcactcc acaccagaca cagccactgc
     9301 ccaccgtcgc cgcgcctgtc ttgagtgcac cgctgggttc tcacgaatgg caacaatcat
     9361 taagccagca tatttcgctg ttcacccgcc aggggcaaca aagtgcagag ttgcgtctgc
     9421 agccacagga tttaggtgaa gtgcaaatct ccctcaaagt ggatgataac caggcgcaaa
     9481 tccagatggt ttcgccgcat cagcacgtac gcgccgccct ggaagcagcg ctgccggtac
     9541 ttcgtacgca gctggccgaa agtggcattc agttagggca aagcaacatc agtggcgaaa
     9601 gctttagtgg ccagcagcag gccgcttccc agcaacagca aagccaacgc acagcaaacc
     9661 atgaacctct ggcgggggaa gacgacgata cgcttccggt tcccgtctct ttacaagggc
     9721 gtgtaacagg caacagcggc gttgatattt tcgcctaacg tcagaggtag caccgtaatc
     9781 cgcgtctttt ccccgctttg ttgcgctcaa gacgcaggat aattagccga taagcagtag
     9841 cgacacagga agaccgcaac acatgactga ttacgcgata agcaagaaaa gcaagcgatc
     9901 gctttggatc ccgattctgg tattcattac cctcgcggcc tgtgccagcg caggttacag
     9961 ctactggcat tcgcatcagg ttgccgctga cgacaaagcg cagcaacgcg tcgtgccctc
    10021 cccggtcttc tatgcgctgg atacctttac ggtcaatctg ggcgatgcgg atcgcgtgct
    10081 ttatatcggc ataaccctgc gactgaaaga tgaagcaacc cgctcgcggc tgagtgagta
    10141 tttgccggaa gtccgtagtc gcttgctgtt actgttttcg cgtcaggatg ctgccgtact
    10201 ggcgacagaa gaaggcaaga aaaacctgat tgccgagatt aaaaccacac tttccacccc
    10261 gcttgttgcc gggcaaccga aacaggatgt caccgacgtg ctgtataccg cttttattct
    10321 gcgataacga catgggcgat agtattcttt ctcaagctga aattgatgcg ctgttgaatg
    10381 gtgacagcga agtcaaagac gaaccgacag ccagtgttag cggcgaaagt gacattcgtc
    10441 cgtacgatcc gaatacccaa cgacgggttg tgcgcgaacg tttgcaggcg ctggaaatca
    10501 ttaatgagcg ctttgcccgc cattttcgta tggggctgtt caacctgctg cgccgtagcc
    10561 cggatattac cgtcggggcc atccgcattc agccgtacca tgaatttgcc cgcaacctgc
    10621 cggtgccgac caacctgaac cttatccatc tgaaaccgct gcgcggcaca gggctggtgg
    10681 tgttctcacc gagtctggtg tttatcgccg tggataacct gtttggcggc gatggacgct
    10741 tcccgaccaa agtggaaggc cgcgagttta cccatacaga acagcgcgtc atcaatcgca
    10801 tgttaaagct ggcgcttgaa ggctatagcg acgcctggaa ggcgattaat ccgctggaag
    10861 ttgagtacgt gcgttcggaa atgcaggtga aatttaccaa tatcaccacc tcgccgaacg
    10921 acattgtggt taacacgccg ttccatgtgg agattggcaa cctgaccggc gaatttaata
    10981 tctgcctgcc attcagcatg atcgagccgc tacgggaatt gttggttaac ccgccgctgg
    11041 aaaactcgcg taatgaagat cagaactggc gcgataacct ggtgcgccag gtgcagcatt
    11101 cacagctcga gctggtcgcc aactttgccg atatatcgct acgcctgtcg cagattttaa
    11161 aactgaaacc cggcgacgtc ctgccgatag aaaaacccga ccgcatcatc gcccatgttg
    11221 acggcgtccc ggtgctgacc agtcagtacg gcacactcaa cggtcaatat gcgttacgga
    11281 tagaacattt gattaacccg attttaaatt ctctgaacga ggaacagccc aaatgagtga
    11341 catgaataat ccggccgatg acaacaacgg cgcaatggac gatctgtggg ctgaagcgtt
    11401 gagcgaacaa aaatcaacca gtagcaaaag cgctgccgac gcggtgttcc agcaatttgg
    11461 cggtggtgat gtcagcggaa cgttgcagga tatcgacctg attatggata ttccggtcaa
    11521 gctgaccgtc gaactgggcc gcacacgaat gaccatcaaa gagttattgc gtctgacgca
    11581 agggtccgtc gtggcgctgg acggtctggc gggcgaaccg ctggatattc tgatcaacgg
    11641 ttatttaatt gctcagggcg aagttgtcgt agtcgctgat aaatatggcg tgcggatcac
    11701 cgatatcatt actccgtctg agcggatgcg ccgcctgagc cgttagtgat gaataaccac
    11761 gctactgtgc aatcttctgc gccggtttct gctgcgccac tgctgcaggt gagcggcgca
    11821 ctcatcgcca ttattgccct gatcctcgct gctgcctggc tggtaaaacg gctgggattt
    11881 gcccctaaac gcactggcgt taacggtctg aaaattagcg ccagtgcttc actgggcgcg
    11941 cgtgaacggg ttgtggtggt cgatgtggaa gatgcacggc tggtgctcgg cgttaccgca
    12001 gggcaaatca atctgctgca taaacttccc ccttcagcac caacggaaga gataccgcag
    12061 accgattttc agtcggttat gaaaaacttg cttaagcgta gcgggagatc ctgatgcgtc
    12121 gtttgttgtc tgtcgcacct gtccttctct ggctggttac gcccctcgcc ttcgcgcaac
    12181 tgccgggtat caccagccag ccgctgcctg gcggtggaca aagctggtcg ctcccggtgc
    12241 aaacgctggt gttcatcacc tcgttgacgt ttattccggc aattttactg atgatgacca
    12301 gtttcacccg catcatcatt gtttttggtt tattgcgtaa cgcgttggga acaccctccg
    12361 cgccacctaa ccaggtattg ctgggactgg cgctgttttt gacctttttt attatgtcgc
    12421 cggtgatcga taaaatttat gtcgatgcgt accagccatt cagcgaagag aaaatctcaa
    12481 tgcaggaggc gctggaaaaa ggggcgcagc cgctgcgtga gtttatgctg cgtcagaccc
    12541 gcgaggcgga tttagggttg tttgccagac tggcgaatac tggcccgttg cagggacctg
    12601 aagctgtgcc gatgcgcatt ttgctcccgg cctacgtgac cagcgagttg aaaaccgcat
    12661 ttcagatagg cttcacgatt ttcattcctt ttttgattat cgacctggtg attgccagcg
    12721 tgttgatggc attggggatg atgatggttc ccccagccac aattgctctg ccctttaaac
    12781 tgatgctgtt tgtactggtg gatggctggc aattgctggt cggttcgctg gcgcagagct
    12841 tttacagcta gagaggcaaa atgacacctg aatcggtcat gatgatgggg actgaagcga
    12901 tgaaagtcgc gctggcactg gctgccccac tgttgctggt ggcgctggtc acgggtctta
    12961 tcatcagtat tttgcaggcc gccacccaga ttaacgaaat gacgctgtcg tttattccga
    13021 aaatcatcgc cgtatttatc gccattatta ttgccggacc gtggatgctc aatctgttgc
    13081 tggattacgt ccgcaccttg ttcactaacc tgccgtatat catcgggtag gccgtacaat
    13141 gatgcaggta acaagcgatc aatggctttc ctggttaagc ctgtacttct ggccgttact
    13201 gcgcgtgctg gcgctcattt ctaccgcacc aattctgagc gaacgcagcg tgccgaaacg
    13261 ggtaaaactg ggtctggcaa tgatgatcac gttcgctatt gccccatcat tacctgccaa
    13321 cgatgttcct gttttttcgt tctttgctct gtggctggcc gtgcagcaga tcctgatcgg
    13381 cattgcgctt ggttttacca tgcaatttgc ctttgccgct gtgcgaaccg ctggtgaaat
    13441 tatcggtctg caaatggggc tgtcatttgc gacgtttgtc gatccggcca gccatctcaa
    13501 tatgcccgtt ttagcgcgta tcatggatat gctggcgtta ctgctgttcc tgacatttaa
    13561 cggtcattta tggttgattt cactgctggc cgataccttt cacaccctgc caattggtgg
    13621 cgaaccgttg aacagcaatg cgtttctggc actcaccaaa gcagggagtt tgattttcct
    13681 taacgggctg atgctggcat taccgctcat tactctgctg ctgaccctga atctggcatt
    13741 aggcttactt aatcgtatgg ccccgcaatt atccattttt gttattggat ttccattaac
    13801 tctgactgtc ggtatctctt taatggcggc attaatgccg ttaattgcgc ctttttgcga
    13861 acatttattc agtgagatgt ttaatttgct ggctgatatt attagtgaat tgccattaat
    13921 ataattccgt aacgtttatc atgttatcct aaggattatc cgaaaaataa tacctacgaa
    13981 catcttccag gatactcctg cggcgaaata tttgttttaa gctcactcac atatcgcaac
    14041 atttacttta ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt
    14101 ccgcctgaaa aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg
    14161 atgaaatatt caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa
    14221 cgattattat ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta
    14281 gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt
    14341 gtgattcaca gcgcccttca gtggtgttta ttaatgagga ctgtttcatc cacgatgctt
    14401 ctaacagtca gcgtatcaag ttcatcatta atcaacatcc caatacgtta tttatcgttt
    14461 ttatggcaat tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aatttattga
    14521 tcagttctaa atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa
    14581 aagagacaac gataacctcg tttttaaata tgccgacgtt atcattgagc cgaaccgaat
    14641 cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga
    14701 atatcaaagc caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacgc
    14761 ataataaaca ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt
    14821 ttgtcaacat gcgctaacac attctgactg gtggtttccc accagtcagg ccgaattaga
    14881 ttactctgct ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat
    14941 gccgagcggg tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac
    15001 tgccagtacc acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt
    15061 caccttcatc gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg
    15121 cagcgccagg cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg
    15181 cggacttaac gactggccgc aacaacagag taacggttgc gaggaaagat gatgaaaacc
    15241 gcaaaagagt acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg
    15301 gcggcgatca atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac
    15361 gttagcgtcg atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg
    15421 gatattcatg acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg
    15481 accctgaggg ggtcgggatg aaacttgctt a
//
LOCUS       DAPOZO010000107        15363 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPOZO010000107 DAPOZO010000000
VERSION     DAPOZO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.107"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            121..315
                     /gene="bfd"
                     /locus_tag="ROI21_003852"
     CDS             121..315
                     /gene="bfd"
                     /locus_tag="ROI21_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDX5112721.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            388..864
                     /gene="bfr"
                     /locus_tag="ROI21_003853"
     CDS             388..864
                     /gene="bfr"
                     /locus_tag="ROI21_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDX5112722.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(861..1328)
                     /locus_tag="ROI21_003854"
     CDS             complement(861..1328)
                     /locus_tag="ROI21_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDX5112723.1"
                     /translation="MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1707..2018
                     /gene="rpsJ"
                     /locus_tag="ROI21_003855"
     CDS             1707..2018
                     /gene="rpsJ"
                     /locus_tag="ROI21_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDX5112724.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2051..2680
                     /gene="rplC"
                     /locus_tag="ROI21_003856"
     CDS             2051..2680
                     /gene="rplC"
                     /locus_tag="ROI21_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDX5112725.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2691..3296
                     /gene="rplD"
                     /locus_tag="ROI21_003857"
     CDS             2691..3296
                     /gene="rplD"
                     /locus_tag="ROI21_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDX5112726.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3293..3595
                     /gene="rplW"
                     /locus_tag="ROI21_003858"
     CDS             3293..3595
                     /gene="rplW"
                     /locus_tag="ROI21_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDX5112727.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3613..4434
                     /gene="rplB"
                     /locus_tag="ROI21_003859"
     CDS             3613..4434
                     /gene="rplB"
                     /locus_tag="ROI21_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDX5112728.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4451..4729
                     /gene="rpsS"
                     /locus_tag="ROI21_003860"
     CDS             4451..4729
                     /gene="rpsS"
                     /locus_tag="ROI21_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDX5112729.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4744..5076
                     /gene="rplV"
                     /locus_tag="ROI21_003861"
     CDS             4744..5076
                     /gene="rplV"
                     /locus_tag="ROI21_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDX5112730.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5094..5795
                     /gene="rpsC"
                     /locus_tag="ROI21_003862"
     CDS             5094..5795
                     /gene="rpsC"
                     /locus_tag="ROI21_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDX5112731.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5808..6218
                     /gene="rplP"
                     /locus_tag="ROI21_003863"
     CDS             5808..6218
                     /gene="rplP"
                     /locus_tag="ROI21_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDX5112732.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6218..6409
                     /gene="rpmC"
                     /locus_tag="ROI21_003864"
     CDS             6218..6409
                     /gene="rpmC"
                     /locus_tag="ROI21_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDX5112733.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6409..6663
                     /gene="rpsQ"
                     /locus_tag="ROI21_003865"
     CDS             6409..6663
                     /gene="rpsQ"
                     /locus_tag="ROI21_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDX5112734.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6828..7199
                     /gene="rplN"
                     /locus_tag="ROI21_003866"
     CDS             6828..7199
                     /gene="rplN"
                     /locus_tag="ROI21_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDX5112735.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7210..7524
                     /gene="rplX"
                     /locus_tag="ROI21_003867"
     CDS             7210..7524
                     /gene="rplX"
                     /locus_tag="ROI21_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDX5112736.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7539..8078
                     /gene="rplE"
                     /locus_tag="ROI21_003868"
     CDS             7539..8078
                     /gene="rplE"
                     /locus_tag="ROI21_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDX5112737.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8093..8398
                     /gene="rpsN"
                     /locus_tag="ROI21_003869"
     CDS             8093..8398
                     /gene="rpsN"
                     /locus_tag="ROI21_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDX5112738.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8432..8824
                     /gene="rpsH"
                     /locus_tag="ROI21_003870"
     CDS             8432..8824
                     /gene="rpsH"
                     /locus_tag="ROI21_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDX5112739.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8837..9370
                     /gene="rplF"
                     /locus_tag="ROI21_003871"
     CDS             8837..9370
                     /gene="rplF"
                     /locus_tag="ROI21_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDX5112740.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9380..9733
                     /gene="rplR"
                     /locus_tag="ROI21_003872"
     CDS             9380..9733
                     /gene="rplR"
                     /locus_tag="ROI21_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDX5112741.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9748..10251
                     /gene="rpsE"
                     /locus_tag="ROI21_003873"
     CDS             9748..10251
                     /gene="rpsE"
                     /locus_tag="ROI21_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDX5112742.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10255..10434
                     /gene="rpmD"
                     /locus_tag="ROI21_003874"
     CDS             10255..10434
                     /gene="rpmD"
                     /locus_tag="ROI21_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDX5112743.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10438..10872
                     /gene="rplO"
                     /locus_tag="ROI21_003875"
     CDS             10438..10872
                     /gene="rplO"
                     /locus_tag="ROI21_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDX5112744.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10880..12211
                     /gene="secY"
                     /locus_tag="ROI21_003876"
     CDS             10880..12211
                     /gene="secY"
                     /locus_tag="ROI21_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDX5112745.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12243..12359
                     /gene="rpmJ"
                     /locus_tag="ROI21_003877"
     CDS             12243..12359
                     /gene="rpmJ"
                     /locus_tag="ROI21_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDX5112746.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12506..12862
                     /gene="rpsM"
                     /locus_tag="ROI21_003878"
     CDS             12506..12862
                     /gene="rpsM"
                     /locus_tag="ROI21_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDX5112747.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12879..13268
                     /gene="rpsK"
                     /locus_tag="ROI21_003879"
     CDS             12879..13268
                     /gene="rpsK"
                     /locus_tag="ROI21_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDX5112748.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13302..13922
                     /gene="rpsD"
                     /locus_tag="ROI21_003880"
     CDS             13302..13922
                     /gene="rpsD"
                     /locus_tag="ROI21_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDX5112749.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13948..14937
                     /gene="rpoA"
                     /locus_tag="ROI21_003881"
     CDS             13948..14937
                     /gene="rpoA"
                     /locus_tag="ROI21_003881"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDX5112750.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14978..15361
                     /gene="rplQ"
                     /locus_tag="ROI21_003882"
     CDS             14978..15361
                     /gene="rplQ"
                     /locus_tag="ROI21_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDX5112751.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         3961 a         3614 c         4093 g         3695 t
ORIGIN      
        1 ttgagctcga aatatttagt cgtaatttca ctcgctgaac tatggcgcaa ataaaactgt
       61 gctattgtaa tcataaccat tctcatttac actttgcgca gaatttgaac gggagcaatc
      121 atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag
      181 tttcatccgc aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt
      241 aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt
      301 aaagagattg cctgaagccc acgcttattt tttgacatcc cgctagcccc atctacgctc
      361 taatagtgga agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc
      421 aataaactat tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg
      481 tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat accatgaatc tatcgatgag
      541 atgaaacacg ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta
      601 caggatttag gcaaactggg gattggtgaa gatgtcgagg agatgctgca atccgacctg
      661 cgattagaac tggaaggcgc taaggatctc cgtgaggcta tcgcgtatgc cgacagcgtt
      721 catgactatg ttagccgtga tatgatgatc gagatccttg ctgatgaaga aggtcatatt
      781 gactggctcg aaactgagct ggatctcatc ggtaaaatcg gtctacaaaa ttacctgcaa
      841 tcacaaataa aagttaaaga ttaacggcct gccagcaaac tatcccagcc tacaacgaaa
      901 cccgcagccg ccagaaatgg cccaaacggc agcgggtttt ttaatgactc ttttcctctc
      961 atgagcagac caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt
     1021 ggcaagaagg tccatgtatg ccaggcacca agggcggcga gaaacttcac atcaccatag
     1081 cctaatcctt ctttatgacg cagtatgcga tacccccagt aaatgacagc gaatgtgccg
     1141 taaccaataa tcgcccccca taacgcatcg gctaaaccgt caggattaca aacttgatag
     1201 aacaaaagac ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt
     1261 gcatcccaga aaaaaagtag ggctgataaa caagcgtaca aaatcagaaa gggaagggta
     1321 gcgtccataa aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag
     1381 caacagagaa atcgctgatt tgttaacctg gttccggtga aaaacgctat cttcacaact
     1441 ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg cgattaattt ctacccgatg
     1501 cttgcgtcag gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc
     1561 aatctgaacc tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac
     1621 gtaagaacgg ttacactctc ccatcaattg taatgggtct gaggagtaat cattttcgtt
     1681 tataaaataa ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag
     1741 cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca
     1801 ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc
     1861 tgatctcccc gcacgtcaac aaagatgcgc gcgatcagta cgaaatccgt actcacttgc
     1921 gtctggttga catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc
     1981 tggctgccgg tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga
     2041 ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga
     2101 agacggcgtt tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt
     2161 taaagacctg gctaacgatg gctaccgtgc tattcaggta accaccggtg ctaaaaaagc
     2221 taaccgtgtg accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg
     2281 tggtctgtgg gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag
     2341 cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg
     2401 tttcgcaggt accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc
     2461 cttgtctcac cgcgttccgg gttctattgg tcagaaccag actccgggca aagtgttcaa
     2521 aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt
     2581 agtacgcgtt gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac
     2641 cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag
     2701 tattgaaaga cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca
     2761 acgaagcgct ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc
     2821 gtgctcagaa gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag
     2881 gcaccggccg tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga
     2941 cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg
     3001 cgctgaaaag catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt
     3061 tctctgtaga agcgccgaaa accaagctgc tggcacagaa actgaaagac atggctctgg
     3121 aagatgtgct gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc
     3181 tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg
     3241 acaaagtcgt aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg
     3301 tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc
     3361 gatggaaaaa tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat
     3421 caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt
     3481 taaagggaaa gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc
     3541 ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg
     3601 aggagtaata caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgtt
     3661 gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa
     3721 aacagcaaat ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt
     3781 ggccacaagc aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca
     3841 gttgttgaac gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac
     3901 aaagacggtg aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt
     3961 cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg
     4021 gttggttcta ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt
     4081 tccgctggta cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg
     4141 cgttctggtg aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc
     4201 aatgctgagc atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt
     4261 cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa
     4321 ggtcgtaact ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag
     4381 acccgcagca acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag
     4441 aggataagcc atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa
     4501 gaaggtagag aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg
     4561 ttcaacgatc tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca
     4621 cgttccggta tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac
     4681 tcgtacttat cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa
     4741 gagatggaaa ctatcgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt
     4801 gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc
     4861 aacaagaaag cggctgtact ggtcaagaaa gttctggaat ctgccattgc taacgctgaa
     4921 cacaacgatg gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc
     4981 ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc
     5041 accagccaca tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc
     5101 agaaagtaca tcctaatggt attcgcctgg gtattgtaaa accatggaac tctacctggt
     5161 ttgcgaacac caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc
     5221 tgactaagga actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga
     5281 gcatccgtgt aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag
     5341 acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca
     5401 tcgccgaagt tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc
     5461 agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc
     5521 gtctgggcgc taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg
     5581 cacgtaccga atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg
     5641 actacaacac ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct
     5701 tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc
     5761 agcctaaaaa gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa
     5821 agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg
     5881 atgttagctt cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc
     5941 agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga
     6001 tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag
     6061 gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa
     6121 tggacggtgt tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc
     6181 cgattaaaac cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga
     6241 gaagagtgtt gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct
     6301 gcgtatgcag gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg
     6361 cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa
     6421 atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct
     6481 atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca tcaagcgtac gaccaaactg
     6541 cacgtacatg acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc
     6601 cgtccgctgt ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg
     6661 taatacagta cgctctctcg atacgaataa acggctcaga aatgagccgt ttattttttc
     6721 tacccatatc cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga
     6781 cctgattttc gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac
     6841 agactatgct gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc
     6901 tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag
     6961 aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca
     7021 ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg
     7081 ttcttctgaa caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc
     7141 gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag
     7201 gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa
     7261 gataaaggta aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattgttgaa
     7321 ggtatcaacc tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc
     7381 atcgttgaaa aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc
     7441 ggcaaggctg accgtgtagg ctttagattc gaagacggta aaaaagtccg tttcttcaag
     7501 tctaacagcg aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact
     7561 acaaagacga agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag
     7621 tccctcgggt cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa
     7681 aactgctgga taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca
     7741 ccaaagcacg caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta
     7801 aagtaactct gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg
     7861 tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact
     7921 acagcatggg tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc
     7981 gcgttcgtgg tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg
     8041 ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa
     8101 gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa
     8161 acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa
     8221 cgctgttctc aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg
     8281 ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa
     8341 ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt
     8401 gtcaccaatt gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat
     8461 gctgacccgt atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc
     8521 caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa
     8581 agttgaaggc gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc
     8641 tgttgtagaa agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga
     8701 tgagctgccg aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt
     8761 tatgactgat cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc
     8821 ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt
     8881 tgatgtaaaa atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg
     8941 tactctcaac gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg
     9001 tgatggttac gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat
     9061 ggttatcggt gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg
     9121 tgcagcggtt aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca
     9181 tcagctgcct gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg
     9241 cgctgataag caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga
     9301 gccttacaaa ggcaagggtg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa
     9361 gaagaagtaa ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc
     9421 acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca
     9481 catttacgca caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt
     9541 agaaaaagct atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt
     9601 gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg
     9661 ttccgggttc caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg
     9721 ccttcagttc taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg
     9781 caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc
     9841 tccttcacag ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt
     9901 aaagcgcgtg aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg
     9961 attaacgtcg cgctgaataa cggcactctg caacaccctg ttaaaggtgt tcatacgggt
    10021 tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg
    10081 cgcgccgttc tggaagtcgc tggggttcat aacgtactgg ctaaagcgta tggttccacc
    10141 aacccgatta acgtggttcg tgcaactatc gatggcctgg aaaatatgaa ttctccagaa
    10201 atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca
    10261 aagactatta aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca
    10321 acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct
    10381 gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg
    10441 cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt
    10501 ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaagtctcgt
    10561 tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg
    10621 ccgaaattcg gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctgtctgac
    10681 ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc
    10741 ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg aagtaactac tccggtaact
    10801 gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa
    10861 atcgaggaat aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg
    10921 tggcttaggc gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg
    10981 tattggctct tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga
    11041 gcaacagcga ggcaccatca ttgaaatgtt taacatgttc tctggtggtg ctctcagccg
    11101 tgcttctatc tttgctctgg ggatcatgcc gtatatttcg gcatcgatca ttatccagct
    11161 gctgacggtg gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg
    11221 taagatcagc cagtacaccc gctacggtac tctggtgctg gcaatattcc agtcgatcgg
    11281 tattgctacc ggtctgccga atatgcctgg tatgcaaggc ctggtcatta acccgggctt
    11341 tgcattctac ttcaccgctg ttgtaagtct ggtcacagga accatgttcc tgatgtggtt
    11401 gggcgaacag attactgaac gaggtatcgg caacggtatt tcaattatta tcttcgccgg
    11461 tattgtcgcg ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga
    11521 cctgcacttc ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt
    11581 tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg
    11641 tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt
    11701 aatcccggca atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt
    11761 cgggggcggt actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca
    11821 accgctttat gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc
    11881 gttggttttc aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc
    11941 aggaattcgt ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac
    12001 cttggttggt gcgctgtaca ttacctttat ctgcctgatc ccggagttca tgcgtgatgc
    12061 aatgaaagta ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat
    12121 ggactttatg gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa
    12181 gaaggcgaac ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa
    12241 aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg
    12301 atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat
    12361 tttttcgcat atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt
    12421 ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt
    12481 acatattaaa tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca
    12541 taagcatgcc gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc
    12601 cattctggct gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca
    12661 aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga
    12721 aatcagcatg agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg
    12781 tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc
    12841 gcgcaaaccg atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg
    12901 cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca
    12961 acaacaccat cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg
    13021 gtggttccgg tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag
    13081 agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag
    13141 gtccgggtcc aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca
    13201 ctaacattac tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc
    13261 gcgtataacg cttcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc
    13321 ctaagctcaa gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg
    13381 cgatcgatac caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc
    13441 gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg
    13501 tgctggagcg tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg
    13561 gtgaaaacct gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct
    13621 tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg
    13681 gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg
    13741 agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa
    13801 agccaacctg gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg
    13861 agcgttctga tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt
    13921 aaagcttagt accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg
    13981 cgcctggttg atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta
    14041 gagcgtggct ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg
    14101 ccgggttgcg cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa
    14161 gaaggcgttc aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga
    14221 gttcagggca aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact
    14281 gcagccgata tcacccacga cggtgatgtc gaaatcgtca agccgcagca cgtgatctgc
    14341 cacctgaccg atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt
    14401 tatgtgccgg cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg
    14461 ctggtcgacg catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt
    14521 gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc
    14581 gatcctgaag aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc
    14641 gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg
    14701 atcctgctgc gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa
    14761 gcagaagcta tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa
    14821 acgcctaacc ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga
    14881 ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg
    14941 gatcacaggt taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg
    15001 tcaactgaac cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact
    15061 ggttcgtcat gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt
    15121 tgagccgctg attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc
    15181 ccgtactcgt gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc
    15241 gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc
    15301 gccgatggct tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta
    15361 atc
//
LOCUS       DAPOZO010000108        15259 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPOZO010000108 DAPOZO010000000
VERSION     DAPOZO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.108"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..972
                     /gene="holA"
                     /locus_tag="ROI21_003883"
     CDS             <1..972
                     /gene="holA"
                     /locus_tag="ROI21_003883"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDX5112752.1"
                     /translation="YLLLGNDPLLLQESQDAIRQVAAAQGFEEHHTFSIDPNTDWNAI
                     FSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTGLLHDDLLLIVRGNKLSKAQE
                     NAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNLELDDAANQVLCYCYEGNLLA
                     LAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWVDALLMGKSKRALHILQQLRL
                     EGSEAVILLRTLQRELLLLVNLKRQSAHTPLRALFDKHRVWQNRRGMMGEALNRLSQP
                     QLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHKPLADVFIDG"
     gene            974..1615
                     /gene="nadD"
                     /locus_tag="ROI21_003884"
     CDS             974..1615
                     /gene="nadD"
                     /locus_tag="ROI21_003884"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDX5112753.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            1639..2250
                     /locus_tag="ROI21_003885"
     CDS             1639..2250
                     /locus_tag="ROI21_003885"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDX5112754.1"
                     /translation="MRLWLIRHDETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRIDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            2510..2827
                     /gene="rsfS"
                     /locus_tag="ROI21_003886"
                     /gene_synonym="rsfA"
     CDS             2510..2827
                     /gene="rsfS"
                     /locus_tag="ROI21_003886"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDX5112755.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            2831..3298
                     /gene="rlmH"
                     /locus_tag="ROI21_003887"
     CDS             2831..3298
                     /gene="rlmH"
                     /locus_tag="ROI21_003887"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDX5112756.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            3329..5230
                     /gene="mrdA"
                     /locus_tag="ROI21_003888"
     CDS             3329..5230
                     /gene="mrdA"
                     /locus_tag="ROI21_003888"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDX5112757.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            5233..6345
                     /gene="mrdB"
                     /locus_tag="ROI21_003889"
                     /gene_synonym="rodA"
     CDS             5233..6345
                     /gene="mrdB"
                     /locus_tag="ROI21_003889"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDX5112758.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            6356..7444
                     /gene="rlpA"
                     /locus_tag="ROI21_003890"
     CDS             6356..7444
                     /gene="rlpA"
                     /locus_tag="ROI21_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDX5112759.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTNSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPATTPATTPA
                     MVTPQATSQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            7584..8795
                     /gene="dacA"
                     /locus_tag="ROI21_003891"
     CDS             7584..8795
                     /gene="dacA"
                     /locus_tag="ROI21_003891"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDX5112760.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            8905..9168
                     /locus_tag="ROI21_003892"
     CDS             8905..9168
                     /locus_tag="ROI21_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDX5112761.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            9269..9910
                     /gene="lipB"
                     /locus_tag="ROI21_003893"
     CDS             9269..9910
                     /gene="lipB"
                     /locus_tag="ROI21_003893"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDX5112762.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDESTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            10170..11123
                     /locus_tag="ROI21_003894"
     CDS             10170..11123
                     /locus_tag="ROI21_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDX5112763.1"
                     /translation="MDSNNQIETCLSRKSSEDKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKSHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHILLL
                     PEGQNFSGLRQRVQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            11332..12297
                     /gene="lipA"
                     /locus_tag="ROI21_003895"
     CDS             11332..12297
                     /gene="lipA"
                     /locus_tag="ROI21_003895"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDX5112764.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(12398..12601)
                     /gene="tatE"
                     /locus_tag="ROI21_003896"
     CDS             complement(12398..12601)
                     /gene="tatE"
                     /locus_tag="ROI21_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDX5112765.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(12730..13518)
                     /locus_tag="ROI21_003897"
     CDS             complement(12730..13518)
                     /locus_tag="ROI21_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDX5112766.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            13614..13997
                     /gene="crcB"
                     /locus_tag="ROI21_003898"
     CDS             13614..13997
                     /gene="crcB"
                     /locus_tag="ROI21_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDX5112767.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(14051..14260)
                     /gene="cspE"
                     /locus_tag="ROI21_003899"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(14051..14260)
                     /gene="cspE"
                     /locus_tag="ROI21_003899"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDX5112768.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(14435..14995)
                     /gene="pagP"
                     /locus_tag="ROI21_003900"
     CDS             complement(14435..14995)
                     /gene="pagP"
                     /locus_tag="ROI21_003900"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDX5112769.1"
                     /translation="MIVSKYVAIFSFVFIQLISVGKVFANADEWMTTFGENITQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         3846 a         3879 c         3737 g         3797 t
ORIGIN      
        1 tatcttttac ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tattcgtcag
       61 gtcgctgcgg cgcaagggtt cgaagaacac cacacttttt ccattgatcc caacaccgac
      121 tggaatgcga ttttttcgtt atgccaggcc atgagcctgt ttgccagtcg acaaacgcta
      181 ttgctgttgt tacccgaaaa cggaccgaat gcggcgatca atgaacaact tctcacactc
      241 accggacttc tgcatgacga cctgctgttg atcgtccgcg gtaataaatt aagcaaagcg
      301 caagaaaatg ccgcctggtt tactgctctt gcgaatcgca gcgtgcaggt gacctgtcag
      361 acaccggagc aggctcagct tccccgctgg gttgctgcgc gtgcaaaaca gctcaactta
      421 gaactggatg acgcggcaaa ccaagtgctc tgctactgtt atgaaggtaa cctgctggcg
      481 ctggcccagg cactggagcg tttatcgcta ctctggccag acggcaaact gacattacct
      541 cgcgttgaac aggcggtgaa tgatgccgcg catttcaccc cttttcattg ggttgatgct
      601 ttgttgatgg gaaaaagtaa gcgcgcgttg catattcttc agcaactgcg tctggaaggc
      661 agcgaagcgg ttattttgtt gcgcacatta caacgtgaac tgttgttact ggttaacctg
      721 aaacgccagt ctgcccatac gccactgcgt gcattgtttg ataagcatcg ggtatggcag
      781 aaccgccggg gcatgatggg cgaggcgtta aatcgcttaa gccagccgca gttacgtcag
      841 gccgtgcaac tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg
      901 tgggcagagc tggaagggtt atctcttctg ttgtgccata aacccctggc ggacgtattt
      961 atcgacggtt gatatgaaat ctttacaggc tctgtttggc ggcacctttg atccggtgca
     1021 ctatggtcat cttaaacccg tggaaacgct ggcgaatttg attggtctga cgcgggtcac
     1081 aatcatccct aataatgttc ctccgcatcg tccccagccg gaagcgaaca gcgtgcagcg
     1141 taaacacatg cttgaactgg cgattgccga caagccatta tttactcttg atgaacgcga
     1201 gctaaagcgc aatgccccct cttacactgc gcaaacactg aaagagtggc ggcaggaaca
     1261 agggccggac gtgccgctgg cgtttattat aggtcaggat tcactgctga cctttccgac
     1321 ctggtacgaa tacgaaacga tactcgacaa tgcacatttg atcgtctgtc ggcgtccagg
     1381 ttacccactt gaaatggcgc aaccgcaata ccagcaatgg ctggaagatc atttgacaca
     1441 taacccggaa gatcttcacc ttcagcctgc cggtaaaatt tatctggctg aaacgccgtg
     1501 gtttaacatc tcggcgacca tcatccgcga acgtttgcaa aacggtgaat cgtgtgagga
     1561 tttattgccg gaaccggtac tgacttacat taaccaacaa ggcttgtatc gctgatacct
     1621 gtcgttacgg agggcatgat gcgactgtgg ttaattcgtc atgatgaaac gcaagcgaat
     1681 atcgatggtc tttacagcgg tcatgcgccc acccccctga ctgcgcgcgg tattgagcaa
     1741 gcgcaaaatc tgcatacgct gctacatggt gtttcctttg acctggtttt atgcagtgaa
     1801 ctggaacggg cacagcatac cgcgcgacgg gttctcagtg atcgccagct ccccgtgcaa
     1861 atcatccctg aactcaacga aatgtttttt ggcgactggg agatgcgaca tcatcgcgac
     1921 ctcatgcaag aagatgccga aaactatagc gcgtggtgca atgactggca gcatgctatc
     1981 cccacgaacg gtgaaggatt tcaggcattt tcacaacgtg tggaacggtt tatcgcaagg
     2041 cttagtgaat ttcagcacta tcagaatatt ttagtcgtca gccatcaggg tgtactgagt
     2101 ctgttaatcg cccgtttaat tggtatgcct gccgaagcta tgtggcattt tcgcattgac
     2161 caggggtgtt ggagcgccat tgatatcaac caaaaattcg cgacgctacg cgtcctcaat
     2221 agccgtgcca tcggggtcga gaatgcatga cttttctgtt tttttacggg taagccgcaa
     2281 cgaccattga cagccccggg caggctgata ttctccgcag ccagactttt ttcgccagac
     2341 acgactttgt agaaattgtt ttacaaaaat gacgatgcaa tctgcggcgc ggggtgggat
     2401 gatagcccac tttcgaaagc cgattcggcg acaattgtcc cgaaatcgcc tctggttcag
     2461 gtatactgac agaccatttt tatctatttg attcacccag ggggaaaact tgcagggtaa
     2521 agcactccag gattttgtta tcgacaaaat tgatgacctc aaaggtcagg acatcatcgc
     2581 cttagacgtt cagggcaaat ccagcatcac cgactgcatg atcatctgta cgggtacgtc
     2641 cagccgtcat gttatgtcca ttgctgacca cgttgtgcag gagtctcgcg cagcgggcct
     2701 gttaccgctc ggcgtagaag gtgaaaacag cgccgactgg attgtcgtgg atttgggcga
     2761 tgtgattgtc catgtcatgc aggaagagag ccgtcgcctg tatgaactgg aaaaactctg
     2821 gagttaatgc gtgaagctgc aacttgtcgc cgtgggaacg aaaatgccgg actgggtaca
     2881 aaccggtttt accgagtacc tgcgtcgttt tccgaaagat atgcccttcg agctgattga
     2941 aattccggcc ggaaaacgcg gcaagaatgc ggacatcaag cgcatactcg acaaagaggg
     3001 tgagcagatg ttggcggccg caggcaaaaa ccgcattgtc accctcgata ttccaggcaa
     3061 gccctgggat acgccgcagt tagccgctga gctggaacgc tggaagctgg atggtcgcga
     3121 cgtcagtcta ctgattggcg ggcctgaagg gttgtcgcct gcctgtaaag cggcggcgga
     3181 gcagagctgg tcgctgtcgg cgcttaccct cccccatccg ttggttcgcg tgctggtcgc
     3241 agagagtctg taccgggcgt ggagcatcac caccaaccat ccttatcacc gtgagtgata
     3301 agggagcttt gagtagaaaa cgcagcggat gaaactacag aactcttttc gcgactatac
     3361 ggcagagtcc gcgctgtttg tgcgccgggc gctggtcgcc tttttgggga ttttgctgct
     3421 gaccggcgtg cttatcgcca acctgtataa tctgcaaatt gttcgcttta ccgactacca
     3481 gacccgctct aatgaaaacc gcattaagct ggtgcctatc gcgcccagcc gcggcattat
     3541 ctacgaccgt aacggtatcc ctctggccct caaccgcact atctaccaga tagaaatgat
     3601 gccggagaaa gtcgataacg tgcagcaaac gctggacgct ttgcgcagcg tggttgatct
     3661 gaccgatgac gatattgctg cattccgaaa agagcgcgca cgttcacacc gtttcacctc
     3721 cattccggtg aaaactaacc tgaccgaagt acaagtagct cgctttgccg tcaatcagta
     3781 ccgttttccg ggtgttgaag ttaaaggcta taaacgtcgt tactatcctt acggttcggc
     3841 gttgacccac gtcatcggct atgtgtcgaa aatcaacgat aaagacgtcg aacgcctgaa
     3901 taatgacggc aaactggcca actatgcggc aacgcatgat atcggtaagc tgggcattga
     3961 gcgttactat gaagacgtgc tgcacggtca gaccggttat gaagaggttg aagttaacaa
     4021 ccgtgggcgt gttatccgcc agttaaaaga agtcccaccg caagccggac acgatattta
     4081 cctgacgctg gatctcaaac tccagcaata tattgaaacg ctgctggcgg gtagccgcgc
     4141 agcggtggta gtcaccgatc cgcgtacagg tggggtgctg gcgctggttt ccacgcctag
     4201 ttatgaccca aacctgtttg ttgacggtat ctccagcaaa gattattccg ccttgctgaa
     4261 cgacccgaat acaccgctgg tgaaccgcgc tacacagggg gtttatcctc ccgcgtctac
     4321 agttaaaccc tatgtggcgg tttcggcatt gagcgccgga gtgatcacgc gcaatacgac
     4381 gctgtttgac ccaggctggt ggcaactgcc aggttcggaa aaacgttatc gtgactggaa
     4441 aaaatggggc cacgggcgtc tgaatgtcac aagatcgctg gaagaatctg cggatacctt
     4501 cttctatcag gtggcctacg atatgggaat cgatcgcctc tccgaatgga tgggtaaatt
     4561 cggttatggt cattacaccg gtatcgacct ggcggaagaa cgttccggca acatgcctac
     4621 ccgcgaatgg aaacagaaac gctttaaaaa accgtggtat cagggtgaca ccattccggt
     4681 tggtatcggt cagggttact ggacagcgac cccaatccag atgagtaagg cactgatgat
     4741 cctgattaat gacggtatcg tgaaggttcc gcatttgctg atgagcaccg ccgaagacgg
     4801 caaacaggtg ccatgggtac aaccgcatga accgcccgtc ggcgatattc attccggtta
     4861 ctgggagctg gcgaaagacg gtatgtacgg tgttgctaac cgccctaacg gtacggcgca
     4921 taaatacttt gccagcgcac cgtacaaaat tgcggcgaaa tccggtaccg ctcaggtctt
     4981 cggtctgaaa gcgaacgaaa cctataatgc gcacaaaatt gccgagcgtt tacgtgacca
     5041 caaactgatg accgcctttg cgccatacaa caatccgcaa gtcgctgtcg ccatgattct
     5101 ggagaacggc ggtgcgggtc cggcggttgg tacgctgatg cgccagatcc tcgaccacat
     5161 tatgctgggt gataacaaca ccgatctgcc tgcggaaaat ccagcggttg ccgcagcgga
     5221 ggaccattaa tcatgacgga taatccgaat aaaaaaacat tctgggataa agtccatctc
     5281 gatcccacaa tgctgctgat cttactggca ttgctggttt acagcgccct ggttatctgg
     5341 agcgccagcg gtcaggatat tggcatgatg gagcgtaaaa tcggccaaat cgcgatgggt
     5401 ctggtcatca tggtggtgat ggcgcaaatt cctccacgcg tttatgaagg ctgggccccc
     5461 tatctctata tcatctgtat tattttgctg gtggcggtag atgctttcgg tgccatctct
     5521 aaaggcgctc aacgctggct ggacctcggt attgttcgtt ttcagccgtc ggaaattgcc
     5581 aaaatagccg taccactgat ggttgcgcgc tttatcaacc gcgacgtttg cccgccatcg
     5641 ttgaagaaca ctggcatcgc gctagtgctg atatttatgc ccacgctgct ggtggctgca
     5701 cagcctgacc tggggacatc aatcctcgtt gcgctttccg gtctgtttgt actgttcctc
     5761 tctggcctta gctggcgtct gattggcgtc gcagtagtgc tggtagcggc gttcattccg
     5821 attctgtggt tcttcctgat gcatgattac cagcgccagc gcgtaatgat gctcctggac
     5881 ccggaatcag acccactcgg cgcgggctat cacattattc agtctaaaat tgctattggc
     5941 tccggcggat tacgcggcaa aggctggctg catggcactc agtcacaact tgaatttctc
     6001 cccgaacgcc ataccgactt tatcttcgcg gtactggcgg aagagctggg attagtgggc
     6061 attttgattc tgctcgctct ctacattctg ctgatcatgc gcgggctgtg gatagccgcc
     6121 agagcgcaaa ccacctttgg tcgcgtcatg gctggcggct taatgctgat attattcgtt
     6181 tatgtcttcg taaatattgg tatggtaagc ggtattctgc cggttgtagg ggttccgctc
     6241 ccactggtca gttatggagg atcggcgcta attgtgctga tggctgggtt cgggattgta
     6301 atgtcaatcc acacccacag gaaaatgttg tcgaaaagcg tgtaagaggt gcgcaatgcg
     6361 taagcagtgg ctcgggatct gcatcgcggc aggaatgctc gcggcatgta caagcgatga
     6421 tggtcagcaa cagacggtaa gtgtaccgca gcctgcggta tgtaacggcc ccatagttga
     6481 aattagcggg gcggacccgc gtttcgaacc actgaacgcg acggcaaatc aggattacca
     6541 gcgcgacggt aaaagctaca aaatcgtgca ggatccgtct cgatttagcc aggcgggact
     6601 ggcggcaatc tatgatgccg aacccggcag taacctgacg gcctctggcg aagcattcga
     6661 tccgacacag ctgacagcgg cccatccaac gcttccgatc cccagctacg ccagaatcac
     6721 taacctggct aacgggcgaa tgatcgtggt gcgcattaat gatcgcggtc cttacggcaa
     6781 cgaccgcgtt atttcgcttt ctcgcgcggc agctgaccgt cttaacacgt caaacaacac
     6841 caaagttcgt atcgatccga ttattgtcgc ccaggatggt tcgctttctg gtcctggtat
     6901 ggcctgtacc acagttgcca aacagactta cgccctgcct gcacctcccg atttaagcgg
     6961 cggcgcggga acaaattcag tgtctggccc gcagggtgac attcttccgg tcagtaattc
     7021 gacgctaaaa agcgaagatc cgaccggcgc gccggtaacc agcagcggtt tcctcggcgc
     7081 accaacgacc ttagcgcctg gtgttctgga aggcagcgaa ccaacacctg caccacagcc
     7141 cgttgttaca gctccggcga caacgcctgc aaccacgcct gcaatggtga caccgcaagc
     7201 cacctcgcaa agcgccagcg gcaactttat ggtgcaagtc ggagccgtta gcgatcaggc
     7261 tcgtgcgcaa cagtaccaac agcaactggg acagaagttc ggcgtccccg gtcgcgtaac
     7321 tcaaaatggc gcagtctggc ggatccagct tggcccattc gccagcaaag ccgaagccag
     7381 taccttgcag caacgtttgc aaaccgaagc ccaattacag tcatttatta ccaccgcgca
     7441 gtagcgtaaa gcaggcatct gatgtgtcaa tttctgtaaa tgacgttaac aaagtcacgc
     7501 ggaaagtcag atgcctgccg gtagtgcatt tgctatagta gggcactttt tttaattcca
     7561 tcacggatgt cgtagttcag accatgaata ccattttttc cgctcgtatc atgaagcgcc
     7621 tggcgctcac cacggctctt tgcacagcct ttatctctgc tgcacatgcc gatgacctga
     7681 atatcaaaac tatgatcccg ggtgtaccgc agatcgatgc ggagtcctac atcctgattg
     7741 actataactc cggcaaagtg ctcgccgaac agaacgcaga tgtccgccgc gatcctgcca
     7801 gcctgaccaa aatgatgacc agttacgtta tcggccaggc aatgaaagcc ggtaaattta
     7861 aagaaactga tttagtcacc atcggtaacg acgcatgggc taccggtaac ccggtgttta
     7921 aaggttcttc gctgatgttc ctcaaaccgg gcatgcaggt tccggtttct cagctgatcc
     7981 gcggtattaa cctgcaatcg ggtaacgatg cttgtgtcgc catggccgat tttgccgctg
     8041 gtagccagga cgcttttgtt ggcttgatga acagctacgt taacgcactg ggactgaaaa
     8101 atacccactt ccagacggta catggtctgg atgctgatgg tcagtacagc tccgcgcgag
     8161 atatggcgct gatcggccag gcgttgatcc gtgacgtacc gaatgaatac tcgatctata
     8221 aagaaaaaga atttacgttt aacggtattc gccagctgaa ccgtaacggt ctgttatggg
     8281 ataacagcct gaatgtcgac ggcatcaaaa ccggacacac tgacaaagca ggttacaacc
     8341 ttgttgcttc tgcgactgaa ggccagatgc gcttgatctc tgcggtgatg ggcggacgta
     8401 cttttaaagg ccgtgaagcc gaaagtaaaa aactgctgac ctggggcttc cgtttcttcg
     8461 aaaccgttaa cccactgaaa gtaggtaaag agttcgcctc tgaaccggtt tggtttggtg
     8521 attctgatcg cgcttcgtta ggtgttgata aagacgtgta tctgaccatt ccgcgtggcc
     8581 gcatgaaaga tctgaaagcc agctatgtgc tgaacagcag tgaattgcat gcgccgctgc
     8641 aaaagaatca ggtcgtcggt actatcaact tccagcttga tggcaaaacg atcgaacaac
     8701 gcccgctggt tgtgctgcaa gaaatcccgg aaggcaactt cttcggcaaa atcattgatt
     8761 acattaaatt aatgttccat cactggtttg gttaaaaatc gaacacttga aagtgtaatt
     8821 tccgtcccca tatactaagc atcagtaaaa aactcccgcc ttctggcggg agttgctatt
     8881 taattacgtt acgccggagc tgacatgaaa accaaactta acgaactgct tgaattccct
     8941 actcctttta cttacaaagt tatggggcag gcgttacctg agctggttga tcaggtggtt
     9001 gaagtggtac agcgccatgc gccaggtgac tacaccccaa cggtaaaacc aagcagcaaa
     9061 ggcaactacc actcggtatc tatcactatc aacgctactc atatcgagca ggttgaaaca
     9121 ctgtatgaag aactgggcaa aatcgatatt gtccgcatgg ttctgtaact cgcttctccg
     9181 ttacccggct cctggtcggg taactcccct ctcctcgctg tgatatactt ttcccccact
     9241 tttactcatt ctccacggag atgccgtttt gtatcaggat aaaattcttg tccgccagct
     9301 cggtcttcag ccttacgagc caatctccca ggctatgcat gaattcaccg atacccgcga
     9361 tgaaagtacc cttgatgaaa tctggctggt cgagcactat ccggtattta cccaagggca
     9421 ggcaggaaaa gcggagcaca ttttaatgcc gggtgatatt ccggtgattc agagcgatcg
     9481 cggtgggcag gtgacttatc acgggccggg gcaacaggtg atgtatgtgt tgcttaacct
     9541 gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg cttgagcaaa cagtggtgaa
     9601 taccctggct gaactgggta tagaagcgca tcctcgggct gacgcgcctg gtgtctatgt
     9661 cggggaaaag aaaatttgct cactgggttt acgtattcga cgcggttgtt cattccacgg
     9721 tctggcatta aacgtcaata tggatctttc accattttta cgtattaatc cttgtgggta
     9781 tgccggaatg gaaatggcta aaatatcaca atggaaaccc gaagcgacga ctaataatat
     9841 tgcaccgcgt ttactggaaa atattttagc gctactaaac aatcctgact tcgaatatat
     9901 taccgcttaa ttgcatacat caatggccca atttacactg ggtcattact cataatacct
     9961 gctaatgctt tacatctcct tcgcgtttgt atattttctt tatcacttgc agaaatgcga
    10021 tgctacgcct tgctccgtaa ctcaccccat tgattaactt attgaaatag ttgattatgg
    10081 tgagcaaaag cgttccgttc caattagaca taagattatt gtaatttttt ttaatattat
    10141 ttttccatga ataaaattgg agtaagtgcg tggatagtaa taatcaaatt gaaacctgtt
    10201 taagcagaaa gtcgtcagaa gataaaccac aaatatttac aaccttacga aatattgatc
    10261 ttaaccttct gactattttt gaagctgtat atgtacataa agggatcgtt aatgcagcga
    10321 aagtgcttaa tctgacaccc tcggcaatca gtcagtctat tcagaaactg cgcgttatat
    10381 tccctgaccc attgtttatt cgcaaaggcc agggtgtcac tcctaccgca tttgcgatgc
    10441 atctacatga gtatatcagt cagggccttg agtccattct tggcgcgctg gatatcgaag
    10501 gaagctatga taagcaacga acgataacca ttgcgaccac cccctcggtc ggagccctgg
    10561 tccttcctgt catctatcgg gcgattaaat ctcactatcc gcagctttta ctgcgcaacc
    10621 cccccatcag cgacgcggaa aaccaactca gtcagtttca aaccgatctc atcatcgata
    10681 acatgttttg caccaatcgt acggtgcaac atcatgttct gttcaccgac aatatggtgt
    10741 taatttgccg cgagggaaat ccactactct ctttagaaga tgacagggag actatcgaca
    10801 acgctgcgca tatactcctg ttaccggaag ggcaaaattt cagcggtctg cggcagagag
    10861 ttcaagagat gtttccggac cggcaaatca gtttcaccag ctacaacatt ttgacaatcg
    10921 ctgcactggt tgccaacagt gacatgttag cgattattcc cagccgtttt tataacctgt
    10981 ttagccgttg ctggccgctg gaaaaactgc cttttccgtc cttaaatgag gagcaaatag
    11041 atttctctat ccactacaac aaattcagcc tgcgtgatcc catcctgcac ggggtaatag
    11101 atgttatccg taatgcattt tgaaaaagta ttgttacagc cagatcacag cgaagaacga
    11161 aaaaatggca caatccaaca acaattttac attttcgcga ccgctttggc tgctttcagg
    11221 tccgtttcaa tgatatactg ccagtcgtta attcaaaaat agttgataat tacaacaatc
    11281 tattgaattg aaacgctttc cttcgtaatt cgcaactgga acacgcacgc tatgagtaaa
    11341 cccattgtga tggaacgcgg tgttaaatac cgcgatgccg ataagatggc ccttatcccg
    11401 gttaaaaacg tggcaacaga gcgcgaagcc ctgctgcgca agccggaatg gatgaaaatc
    11461 aagcttccag cggactctac acgtatccag ggcatcaaag ccgcaatgcg caaaaatggc
    11521 ctgcattctg tctgcgagga agcctcctgc cctaacctgg cggaatgctt caaccacggc
    11581 acagcaacgt ttatgatcct cggcgctatt tgtacccgcc gttgtccgtt ctgtgacgtt
    11641 gcccacggtc gcccggtagc tccggatgcc aatgaaccag tgaaactggc gcagaccatt
    11701 gccgatatgg cgctgcgtta tgtggttatc acctccgttg accgtgatga cctgcgcgat
    11761 ggcggtgccc agcactttgc ggattgcatt actgccattc gggaaaaaag cccgcaaatc
    11821 aaaattgaaa ctctggtgcc ggatttccgc ggtcgtatgg atcgtgctct ggatattctg
    11881 accgccacac caccagatgt gttcaaccat aacctggaaa acgtaccgcg tatttaccgt
    11941 caggtacggc ctggtgcaga ttacaactgg tcgctgaagc tgctggaacg ctttaaagaa
    12001 gcgcatccgg aaatcccgac caagtctggt ctgatggtgg gtctgggtga aaccaatgaa
    12061 gaaattattg aggtaatgcg cgacctgcgc cgtcatggtg tgacgatgtt aacgctgggg
    12121 caatatttgc agccaagccg ccatcacctg ccggttcaac gttacgttag cccggatgag
    12181 ttcgacgaaa tgaaagccga agcgctggcg atgggcttta cccatgctgc atgcggtccg
    12241 tttgtccgct cgtcttacca cgccgatttg caggcgaaag ggatggaagt taagtaatcc
    12301 tgtaataatt acttacatct gtctgataaa aaacccgctt tttgaagcgg gttttttgta
    12361 tcgaacaaga tattgaggga gcgtcctgct cgccacgtca ctctttatga gagagctttt
    12421 cagcctgaag atcaacgtct gcgccttttt tcgccgcagc atcgtcatca ttcatcgcct
    12481 tcttgaaccc tttaatggcc gctccaaggt ctccgcccag cgtacgtaac ttcttagtcc
    12541 caaacagcag aacgaccagc gccgcaacta ccagcagttt ggtaatacta atctcaccca
    12601 tagatacctt cttgacatat aaccggcaac agaacgcgcc ttaaaaacca gagaatatta
    12661 acggtcattc gacgcgcgca cacaatagca atctgtaaca aggtgaatca agcgccgagt
    12721 aaaaaaacat cataataatt gcggcggcgc aaagcgacgg ttgtttaaaa cgggaagttg
    12781 cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc ataatgagtg caggcatttc
    12841 tgacgctgcc gcaatggtga cgccaaaggg atctataatc cggctttgcc cgatattttt
    12901 gttcccgcac tcccccgccg ccaccatata acaggtggta tccagcgcac gagcggcaag
    12961 caacgttgac cagtgatgct ctttgagcgg cccacgaacc caggcggcag gaagtaccag
    13021 gatttcagct ccctgtaatg cctgtgccag cgccagctct ggaaagcgta agtcataaca
    13081 ggtcatcaga ccgaccttca tcccttccac ctccagtaac ggagctattt cattaccagc
    13141 atcaacatgg cgtgattcct gaatggcaaa tgcatcatag agatgcagtt tggcataacg
    13201 ggcgacgatg tttcctgcct gtagcgccac cagcatattc catgcgcgcc ccggcgttga
    13261 aggaacatga atcgtcagaa ttgtcgtcat catgttacgt ttactttctc gccgtaaacg
    13321 tccgaggaat tcgccttcca gcagctgtgc tgatttaacc gatagatctg catcatgatc
    13381 atcgcgcgcc agcaatgctt ccggcaggac aaacagcgat acgtcgtttt ccgccgcctg
    13441 cgccatcaac gaggcacaaa tctcagcgtt cttttcccac acagatgtaa cagcaaactg
    13501 tcctgcggca accagcattg ccacctcctc ctgtcagagt cgtaaaattc gccaacggcg
    13561 attattcggc gttacactta tcactcatac aaatcaaata gcaggatttt gcagtgttac
    13621 aacttctttt agcagttttt attggcggtg gtacgggaag cgtggcgaga tggctgttaa
    13681 gtatgcgatt taacccgctg catcaagcga ttccgttggg gacgctggct gcaaacctga
    13741 ttggggcatt catcatagga atgggattcg cctggtttag caggatgacg aacattgatc
    13801 cagtgtggaa agtattaatc accaccggat tttgtggcgg tctaacaacc ttctcaacat
    13861 tttcggcaga agtggtgttt ttgttacaag agggccgctt tggctgggcg ttactgaacg
    13921 ttttcgtcaa ccttttgggg tcttttgcca tgaccgcact ggctttctgg ctgttttccg
    13981 cctcaaccgc acactaaaga ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg
    14041 ctgacgtatc ttacagagcg attacgtttg cagcagaagg gcctttggca ccgttagtga
    14101 tttcgaactc tacgcgctga ccttcagcaa gagttttaaa accattagtc tggattgcag
    14161 agaagtgtac gaacacgtct ttgctgccgt cttccggagt aatgaaaccg aatcctttgg
    14221 actcattaaa ccacttaacg ttacctttaa tcttagacat caaaattacc tttacatgaa
    14281 aaatagacac aaatgctgtg tcggttacca gtacaccaat tgtggtacgc tttgtccagt
    14341 gcaactttac taaaaagtga taaaagtcgc gttatttttg atgttaatta ttttacccat
    14401 tgattttaaa gagtttacta aaatttcatt tgtctcaaaa ctgaaagcgc atccaggcaa
    14461 agtacacatt gccattgtta taggtacccg gaatgtaggt catctgaaaa gtcactggac
    14521 cataacccac agaggccaat ggcagtagaa ccgggagagg gatgtaattc cagttatcgc
    14581 gtgccgttac gccagcggtg aatcccagac ctaaatgaaa attttcatcc gccagcggtc
    14641 gccaggtact ttcccatccg tatccggcaa tcggttccca tttgttccac gagtccttaa
    14701 atgccatggc atacaggcca tgccagtttc ctttttcatc ccaacgcgac aggccaaaac
    14761 cgccacccca cggtcgctcg ttatagcgat cggttttttc tttgtcgtaa gcgaaacgag
    14821 catgccaggt gatggcagga atatataaat cataatgttc tggctgttgc caggtttgtg
    14881 taatattttc tccaaacgtt gtcatccact catctgcgtt agcaaaaact ttaccaacgc
    14941 tgattaactg aataaaaaca aaggaaaaga tagcgacata tttactcacg atcatttgtg
    15001 accataaaac atttatcaaa aatctactac tagcatagca aagctacaat taacataacc
    15061 ttaatagccc caacataaag aacaatctga atagctgctc tatttatttc cgcttgttta
    15121 agttaaatat ttccgcgcat cgattaaaga tgatgtatcg ttttctgcga tgggaatagt
    15181 cataaaaaaa ccaagtcttt tttgatgaca aaatacaacc aagaaaaaat tactttattt
    15241 tttaatagga tacaaaaaa
//
LOCUS       DAPOZO010000109        15154 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPOZO010000109 DAPOZO010000000
VERSION     DAPOZO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.109"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..1038)
                     /gene="curA"
                     /locus_tag="ROI21_003901"
     CDS             complement(1..1038)
                     /gene="curA"
                     /locus_tag="ROI21_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDX5112770.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPSEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDELVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLTKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVFGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1219..1737
                     /gene="mddA"
                     /locus_tag="ROI21_003902"
     CDS             1219..1737
                     /gene="mddA"
                     /locus_tag="ROI21_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDX5112771.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTLAGYPVLVSEENGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1734..2183
                     /locus_tag="ROI21_003903"
     CDS             1734..2183
                     /locus_tag="ROI21_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDX5112772.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQSSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMEGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(2184..2417)
                     /locus_tag="ROI21_003904"
     CDS             complement(2184..2417)
                     /locus_tag="ROI21_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDX5112773.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREERYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2503..2676)
                     /gene="ortT"
                     /locus_tag="ROI21_003905"
     CDS             complement(2503..2676)
                     /gene="ortT"
                     /locus_tag="ROI21_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDX5112774.1"
                     /translation="MSLYQHMLVFYAVMAAISFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2871..2966
                     /gene="yncL"
                     /locus_tag="ROI21_003906"
     CDS             2871..2966
                     /gene="yncL"
                     /locus_tag="ROI21_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDX5112775.1"
                     /translation="MNVSSRTVVLINVFAAVSLFTLISMRFGWFI"
     gene            complement(3063..4487)
                     /gene="patD"
                     /locus_tag="ROI21_003907"
     CDS             complement(3063..4487)
                     /gene="patD"
                     /locus_tag="ROI21_003907"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDX5112776.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDDSTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4509..5303)
                     /locus_tag="ROI21_003908"
     CDS             complement(4509..5303)
                     /locus_tag="ROI21_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5112777.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5293..6234)
                     /locus_tag="ROI21_003909"
     CDS             complement(5293..6234)
                     /locus_tag="ROI21_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5112778.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLFGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(6235..7248)
                     /locus_tag="ROI21_003910"
     CDS             complement(6235..7248)
                     /locus_tag="ROI21_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5112779.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEKLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(7266..8411)
                     /locus_tag="ROI21_003911"
     CDS             complement(7266..8411)
                     /locus_tag="ROI21_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDX5112780.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTSLIPNWKTLDPRVVKGDWFNVSGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGKKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(8656..10062)
                     /locus_tag="ROI21_003912"
     CDS             complement(8656..10062)
                     /locus_tag="ROI21_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDX5112781.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGHIIARPQSGYYVATQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKAMELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLSQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDHHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALAHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(10141..10557)
                     /gene="hicB"
                     /locus_tag="ROI21_003913"
     CDS             complement(10141..10557)
                     /gene="hicB"
                     /locus_tag="ROI21_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDX5112782.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(10603..10779)
                     /gene="hicA"
                     /locus_tag="ROI21_003914"
     CDS             complement(10603..10779)
                     /gene="hicA"
                     /locus_tag="ROI21_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HDX5112783.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            11001..11231
                     /locus_tag="ROI21_003915"
     CDS             11001..11231
                     /locus_tag="ROI21_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDX5112784.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(11323..13284)
                     /gene="rlhA"
                     /locus_tag="ROI21_003916"
     CDS             complement(11323..13284)
                     /gene="rlhA"
                     /locus_tag="ROI21_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDX5112785.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERVDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVSEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLAGY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(13357..13893)
                     /gene="sutR"
                     /locus_tag="ROI21_003917"
     CDS             complement(13357..13893)
                     /gene="sutR"
                     /locus_tag="ROI21_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDX5112786.1"
                     /translation="MENLARFLSTTLKQLRQHRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGGIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            13946..>15154
                     /locus_tag="ROI21_003918"
     CDS             13946..>15154
                     /locus_tag="ROI21_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDX5112787.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDDQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTNDVVFKPVLPTYITPD
                     FAFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNR"
BASE COUNT         3692 a         4026 c         3706 g         3730 t
ORIGIN      
        1 ttaatcatca cccgccacgc ggatcaccac tttgccgaag tttttaccct tcagcaggcc
       61 gataaatgtc tgtggcgcat tctctaaacc gtcagtaatt tcttcgcggt agtggatttt
      121 atcctctttc acccattgcc ccatctcctt ctgaaactca tggatgcggt gaccataatc
      181 ctgagcgata ataaatcctt gcaagcgaat acgttttttc agcaccgtag ccatcagcag
      241 aggtaaacga tccggaccgg gtggtagctc tgtagcgtta tagctgctca ctaagccgaa
      301 gacgggaatg cgcgcagatg tattaagtaa cggtagcacc gcatcaaata ccttaccgcc
      361 cacgttttca taatagatat caataccttt tgggcacgct ttcgtcagtt gttcggcaaa
      421 atcatccgca tggtgatcaa ggcaaacatc aaaacctaac acctcggtag catggcggca
      481 tttttccgcg ccaccggcca cccccaccac cctgcaacct ttaagtttgc cgatttgacc
      541 caccgtcgcc cccacaggac ctgtcgccgc agcaacaacc aatgtttcgc cctctttggg
      601 ctgaccgata tccagcagcc ccatataagc ggtaaagcct ggcatcccca gcacacccag
      661 cgaccacgat ggattttgcg gatgatcgcc aagtttcacc agttcatcac cactggatat
      721 gtcatagtcc tgccatccac tgtagcccag cacccagtcg ccaggctgat aatcaggatg
      781 attcgactcc acgacacggc tcaccgtacc gccgaccatc acgccgccaa tatcaacagg
      841 cggtgaataa gatggctcat cgctcatacg tccacgcata tacgggtcca gggacaaata
      901 aactgtgcgc agtaacacct gtccttcact cggtgtggcg acatcatctt cttcaagacg
      961 aaagttctcc ggaaccggcg cgccatgcgg acgcgaggcc agaacccaac gtcgattacg
     1021 ctgcttttgt tgccccataa tgctctcctg tgcttttggc atgaatccat caatagtagc
     1081 aggctcgcag gaatggtttt gtttgcagcc gtcacatccc ctctgcaaca cgcaataatt
     1141 tcatcgagga atccgttgct tcgggctgtt ttcgatagta ttgataacgt catcatcatt
     1201 atttaatcga ggccaaacat gtccatccgt tttgcccgca aagccgactg tgctgccatt
     1261 gcggaaattt ataaccacgc cgtgttgtat acggcggcta tctggaatga ccaaacggtg
     1321 gatgcagata accgcattgc ctggttcgaa gcgcggactt tagcaggtta tccagtgctg
     1381 gtgagcgagg aaaacggcgt agtgacggga tatgcctcgt ttggcgactg gcgtagtttt
     1441 gatggttttc gccataccgt ggaacattcg gtttatgtcc atcccgatca tcaggggaaa
     1501 ggtctggggc gtaaattgtt aagccgattg attgatgaag cgcgggattg cgggaagcat
     1561 gtcatggtcg ccgggatcga atcgcaaaat caggcctcgc tgcatctgca ccagtcgctg
     1621 ggatttgtcg tcaccgcaca aatgccgcag gtaggcacta aatttggtcg ttggctggat
     1681 ctgacattta tgcagttgca actcgacgag cgcactgaac cggacgcgat tggatgaatc
     1741 agtcgcttac ccttgctttt ttgattgccg ccggaattgg tctggtggtg caaaacacgc
     1801 tgatggtgcg catcacccag agctcctcta ccattctcat cgccatgttg ctgaactctt
     1861 tggtaggaat tgtgctgttc gtttctattt tgtggtttaa acaaggtatg gaggggtttg
     1921 gtgaactggt gtccagcgtg cgctggtgga cacttatacc tggcttgctg ggatcgtttt
     1981 ttgtcttcgc cagtatcagc ggatatcaga atgtgggagc ggcgacaact attgcggtac
     2041 tggtcgccag ccagctgatt ggcggtctgg tgctggatat cttccgcagc catggcgtac
     2101 cactacgagc gttgatcggc ccaatttgtg gcgcaatttt acttgtggtt ggcgcctggc
     2161 tggtggccag acgctcattt taatcagagg atggtgccgc ctttggttaa ctgttcatgg
     2221 cgcgcttcca tatcttcttt atgtttgcga ccgtgatggg cgatcgctgt gcgcagacgc
     2281 tgctgctggg tatagcgttc ttcccgactt aactccgcat catcgctcag ggcgattaat
     2341 aattcgttca tatgggcaat gacgctctct tcaatcgcgg catccacgcg cgcgatgact
     2401 tcatccagat gcgacatact ttctccttag ccacttgtga aatttatcga ttcatccaaa
     2461 tgatataagt ctgcgtggtg ccgtcaagcc agtgagtaag atttaaaaca gtgaaaacaa
     2521 cagcgccacc gggaaactca tcggccatgt tgcccccacc agaaaggcgc ttaagaaacg
     2581 gatgcgtttc ttatcgtgag aaagaaacca ggtgataaga aatgagattg ctgccataac
     2641 cgcataaaaa acaagcatat gttgatagag agacatgtac gggcgtccag ccagatgaat
     2701 aaaacgcgcg caaatatgcg tcattttttg acgtacatca agttttttaa ccggtttgag
     2761 aaaaaaacat caaacagcaa cttgccctta tccatcttca gaatgagtat tattgcggat
     2821 tttcttaacc cgtactatac acataggggc aatgataaaa ggtggcaaaa atgaatgttt
     2881 ccagtagaac tgtagtactg ataaatgtct ttgctgctgt tagtttgttt actcttatct
     2941 ctatgagatt tggctggttt atttgatgta gtgattttgc acgcagcacg tactgtcaga
     3001 gaaatcagtg ctctgacagt acgcaagatc ttctcatact cttacgatct gcaatattat
     3061 ccttaatgtt taaccatgac gtggcggacg acggtgtaat cctccagccc ataaagtgac
     3121 atatccttgc cgtaaccaga aagtttctgc ccaccgtgcg gcatttcact taccagcatg
     3181 aaatgggtat tgacccaggt acaaccatat tgcagccgcg cgctgacgcg atgcgccctg
     3241 cccacatctt tcgtccatac cgaggatgca agtccatact ggctgtcatt tgcccagttt
     3301 accacctgtt cttcattgtc gaagagcgta acactcacta ctggaccaaa tacctctttt
     3361 tgcacgatgg catcgtcctg taatgcgcca gccagtagcg tcggcgcata gtaatagcca
     3421 ttacccttgc gcttttcacc gccagtgatc actttgatgt gccctgtcgc tttcgcctct
     3481 tctaccgcct tgctgacgcg ttcgagatgc gccagcgaac ttaaaggtcc aagctccgta
     3541 gagtcgtcat ccggcgcacc cgattttaac gttgccactg cagcaccaag tttttccacc
     3601 agcgtatcgt aaatgccttt ttgcgcatag atccgacaag ccgctgtaca atcctgtccg
     3661 gcattgtaat agccaaaagt acgtacacct tcgaccaccg cttcaatatc cgcatcatca
     3721 aaaactatca ctggcgcttt gccaccaagt tccatatgag tacgctttat ggacggcgcg
     3781 gtatggctga tgatgtgctc gccggtggcg atagagcccg tcagcgacac catccgcact
     3841 ttgggatgac cggtcagcgg atcgcccacc gttttgcctc tgccaaacag tacgttaatc
     3901 acgcctgccg ggaagatatc tttcgccagc tctgccaact tcaacgcggt cagcggggta
     3961 atttctgatg gtttaagcac tacacagttc cctgccgcca gcgccggagc cagtttccac
     4021 gcggccatca tcagcggata attccacggt gcgatcgaag ccacgacccc caacggatcg
     4081 cgacggatca tcgaagtatg accttcaaga tattcacctg ccgccagacc attcagacag
     4141 cgcgccgcac cagcgaaaaa gcgaaaaaca tcgacaatcg ccgggatttc atcattgaac
     4201 gcactatgca gcggtttgcc acaattacgg gactccagtt cggcaaaaac ctgaccattt
     4261 tcttcgataa catcagccag tttcagcaga cattccgcac gcactttcgg cgtggtttgc
     4321 ccccattcgg caaatgctgc atctgccgcg cgcacagcag catcgacctg ctctgcggat
     4381 gcctcggcaa tctccagtaa aacgtccccc gttgccggat tatagacagg ctgtttttcc
     4441 ccttcgccgc taaccagttc tccgttaatc agtaacttat gttgcatagc atattcctgt
     4501 atcagtggtt attgaccatt gtcgccttcg cgggttagcc accaggcccc caggatcggc
     4561 aaggttgtta ccaacataac cagcagtgcc accacgttgg ttaccggtac atcacgcggt
     4621 cgcccaagct gattgagcaa ccacaacggt aacgttcgtt catgacctgc cgtaaaggtc
     4681 gtcacgatga tttcatcgaa cgacaaggca aacgccagca ttcctcctgc cagtaacgcc
     4741 gaactgagat tcggcaacac tacgtagcgg aaggtttgcc agccattggc cccaagatcc
     4801 attgacgcct caaccagact ccacgaggtg cggcgaaaac gggcgatgac attgttaaac
     4861 accacaacta cacaaaaagt cgcatgaccg accacgatgg tgaaaaatcc cggctccaga
     4921 ttgatggttt taaaggcggt taacaacgcc agaccagtga caatgcccgg cagcgcaatg
     4981 ggcagcagca gtaacagcga aatggcgttt ttgccgaaaa agtctcgtcg ccacagcgcg
     5041 gcagcggcca gcgtccctaa caccagcgcc attaatgtcg ccagcgccgc cactttaagt
     5101 gacagtgtca cggcatcaag aatatcacta cgctgtgctg ccacgctaaa ccagcgcagc
     5161 gtcaggccct gcggtggaaa actaaacgcc gcatcttcag tgttaaacgc ataggcggcg
     5221 ataatcagga tgggaaaatg taggaaaacc acgccgcccc aggccgccag tttgaggaaa
     5281 aacggtgcgc gttcagagtg catcgaacgc tcccagacgt ttcacgaacg ccaggtacag
     5341 tgcgatgaga acaatcggca ccagggtgaa tgccgccgcc atcggcatat tgccaatcgc
     5401 tccctgctgg gaataaacca tattgccgat aaaatatcct ggagggccaa ccagctgcgg
     5461 aacaataaaa tcgcccagtg tgagtgaaaa ggtaaagata gagccagcgg caataccagg
     5521 gattgccagc ggcagcacca catagcgaaa ggtttgccgt ggacgcgcgc caagatcagc
     5581 cgacgcctgc aacaatgacg gcggcaaacg ctcaagcgcc gcctgcacgg gcaggatcat
     5641 gaacggcagc cagatataga gaaacaccag aaagcgcccc agcccggaag ttgacagcgt
     5701 attgccgccc accgcaggta atgtaaggaa cgcagtcagc agtggttcca gcccaagatg
     5761 ttgtaaaaac cactgagcca cgccatcttt tgccagcaat aacgtccagg cataggcttt
     5821 aacaatgtag ctcgcccaca tcggcaacat taccgcaata taaaaaaacg ctttcatttt
     5881 cccgctggta tagcgcgcca tataccacgc cattggaaaa gccagaatgg cgctggcgat
     5941 agtgactgcc acagccatag tcagcgtgcg gagaatgatg tcgtaattcg ccggattaaa
     6001 cagcgcacgg atattcgcca gcgtcagttc cggcgttacc gacatggtga aatcgtcaaa
     6061 ggtataaaat ccctgccata acagtgtcag cagcgagccg aaatagacaa tgccaaacca
     6121 cattagcggg ccaaacagca gtaaaaacag ccccagcccc ggattacgcc agaagaaacc
     6181 ggacacctta cccagacctg gacgtgaagg tgattgcaat acattcatcg ccattcacct
     6241 ctcctcaacc agcggcacca tcacatcacg cgaccaggaa accatcacct gttgtccggg
     6301 cgtgagcgtg gcaggcagtt cttcgcctgt catattggcc tgactcacaa gcagtttttc
     6361 accgccgttc aatttcagtt caaaacgagt tgccgcgccc tgatattgca ccgcctggat
     6421 cgtgccattg gcctgcagtt caccaggggt gttgaggcgg atatgttccg gtcgcagggc
     6481 gaagcttccc gtcatgccac aaagtttctc tgccatcagt ccatcaaaaa cattcgatgt
     6541 accaacgaac ccggcaacaa acggcgtgcg cgggcgcata tagagatcgc gcggggaatc
     6601 gacctgctca atgcgtccat tattgaaaac cgcaacacga tcggacatcg ataacgcttc
     6661 gccctgatcg tgggtaacga agataaaagt gataccgaga gactgttgca gctttttcag
     6721 ttccagttgc atctgctcac gcaatttgag atccagtgcg ccgagcggtt catccaacag
     6781 caatacgcgc ggttcattca ccaatgctct ggcgatagca acccgctggc gctgaccacc
     6841 agaaagttgt gacggtttac gttgctgtac aaaccccaac gccactttct ccagcgcctc
     6901 ttgcgccatt gcgtgccgct gctttttatt cacgcctttg accatcagcc cataggcgac
     6961 attgtcaaga atcgacatat gcggaaatag cgcgtagtcc tggaagacag tattcacgtc
     7021 ccgctcccac ggtggcagat tgctggcggg tttaccaaag atagagatag ccccgccgga
     7081 aagcttttcg aagccagcaa tcaggcgcag gcaggtggtt ttgccggagc cggacggccc
     7141 cagcatagag aagaactcac catcttttat cgcaatactg acgccatcta ctgcgcgcac
     7201 gtcaccgtac aaccgcgaga cgttgtcaaa ctccactgcg tacgtcataa aacaccccag
     7261 cgaagttagc gaccgcccat aatggcaatg taatcctgcg tccagcgact gtagggaaca
     7321 aacttgcccc cttctgctat aggtgttttc cagaaggcga ttttatcgaa atagttaaaa
     7381 ccgttggttt cacaaccttt tttgcctaat aacggactgg ctttacaccc ttccggcact
     7441 accggtaacg agccaaacca ggccgccaca tcgccctgca cttttggcgt taatgaccag
     7501 ttcatccatt tgtaggcgca aaccggatgt ttcgcttcac tatgtagcat ggtggtgtca
     7561 gcccaaccgg taacgccctc tttcgggaat acggtcgcga caggctggcc ttcggctttc
     7621 agggcgttgg cctgataggg ccatgcactg gaagcaacca caccctcgtt tttgaaatcg
     7681 ctcatttgta cggtagtgtc atgccagtag cgatggatca aactatgttg atcgcgcagc
     7741 actttcagca ccgcctggta ctgttcttcg gtgagttgat acggatcgct gatgcccaac
     7801 tgaggctgag tggctttaac gaacaacgca gcgtccgcaa tgtagatagg gccatcataa
     7861 gcctgaacgc ggcctttatt gctcttgccg tccggcagat tctgctcaac aaaaaccact
     7921 tgccagctat ccggcggcgt cgggaaggtt ttagtgttgt acatcaacag gttcggaccc
     7981 cattggtaag gtgtgccata aactttgcca ctaacgttaa accagtcgcc tttaaccacg
     8041 cgcggatcga gcgttttcca gttgggaatc aatgaggtat taatcggctg cacgcgtttg
     8101 cccataatca ggcgcagcga ggcatcgccg gatgccgtaa ccagatcgta accccctttg
     8161 gtcatcagac tgaccatttc atcggaagtc gcggcggttt tcacattcac cgcgcagcca
     8221 gtctcttttt cgaattgcgt tacccagtcg tattgtttat cagtttgtcc gcgttcgatg
     8281 tatcccggcc aggcgataat atccagtcgc ccttccggtt tatctaaatt ggtaggtggt
     8341 tcggcggcgt gagcggtcat tattgtcatg gtgagcgcac acaggctgct gcgggcaaat
     8401 gtcttgctca taaagtctta ctcctgtcgt aattaattgt tcagccgccg tgccgtaaaa
     8461 atatctcctt gtttaaccat agacggcggc ttgcaggcac accttcgccc ctgagaaaat
     8521 ttaatcaggt tgtgagctac cgcgcattat gccgtctgta aggttatctt ctggagtata
     8581 gacaaggagt taggaaatat gactgctatt cgaaattcat attacccaac ctgagaggaa
     8641 tttaattaaa cgctattaca gccgttcttg aataagtttg cctaattgtt taaccgcctg
     8701 ttcttcacgt tctccccact gccacgcggt attaaaacgg aaaaaacgtg accagttttc
     8761 accggtagaa aacattttac ccggcgcaat actgatatga tgcgccagtg ccgccaggct
     8821 taattcgccc gcatctaacg gttcgggaag ctccagccag agaaaataac cgctgtcatt
     8881 atgatgaatt ttcacttccg caggcagata acgcagcagt gcttgccagg cacgttgttt
     8941 acgttccgca agctggcgac gcaggcgacg aagatgggcg tcgtatcggc gcgtggaaag
     9001 gtaatccacc agagcaagtt gcatcggtga gctggtggaa agcgtactca tcaactgcaa
     9061 gcgttgaatt ttacgtgcat gttttccggc ggcgacccaa ccaatacgaa aaccaggcac
     9121 cagacatttc gaaaacgaag agcaatgcaa aacaccatcg tggtgatccc acgctttcgc
     9181 aggcagcggt ttttcccgtc caaaataaag ttcgctgtaa acgtcatctt caatcagcgt
     9241 tacgttgtac tgactgagca acgctaccag ccgcgctttt ttctgcggcg ttaaggtaaa
     9301 tccgagtgga ttctggctat tagtcatcag ccagcacgct ttcaccggat actcctgcaa
     9361 cgccagttcc atcgccttga gatcaatccc ttctttaacg tccgtcgcca ccgataacgc
     9421 cttcagccgt aaccgctcca gcgcctgcaa cgcgccgtag aaacaaggat tctctactat
     9481 cacccaatcg cccggttcag tgaccgcttg taaactgagg tttaatgcct ctaacgcccc
     9541 ggcggtaatg acaatttcat caggagaaat agtgatgccc tgtaaggcat agcgacgagc
     9601 aatagcctga cgcagttctg cgtttcctgg cggtaagttt tcaatcacgc tcatcgccgt
     9661 ggcggttttg cttacctgcg ccagcgagcg gttgagttgt tggaggggga aaagtcgcgg
     9721 gtcgggaaag gccgaggcaa acggaactac cgacggatcg cgactggcct gcaacatatc
     9781 aaaaatatag gtgttgatat cgactgcttc atctcgagtg actggaatga ctggcgcttt
     9841 cggcattttt attgcctgtg tcgcaacgta ataacccgac tgcggtcgcg cgataatatg
     9901 tccctgactt tcgagcaact gataggcatg gctcacggtc ataaagctca tgccagaaag
     9961 cgccacctgg tcacgcaacg aaggcaaacg atctccgggt tgccagatac ccgacgcaat
    10021 ctgctcgcgt aattgttctg caagctgctg gtattttttc attatcgaat cgtaattatg
    10081 tgcagatgat tcggcagtct atatcagttg ttaaaaatga caacttttca ttaactgtaa
    10141 ttaaaccatc accagcgata actctttgcc aagcgccttt gccgcgagct ggacggcgtc
    10201 gatttttgtc gcatggtgca aattaaatag gcgagtaatc tcctgtttag gtttgccaat
    10261 tcgtctggct aactcttgct gagtgatttc tgactgtaaa aaagcattta acagcaatac
    10321 cttagaggcg acgctcaaag gcacttcaat aaagtgatcg tgactattta atggcgaagg
    10381 taacgggata agctcgttat cttcaaaata aaaatcaaat gcggtcagta aagcatcttt
    10441 tgccgcttcc atcgcttcag caactgtttc gccctgggtc aacgcttcag ggatatccac
    10501 aaaagaaacc atataaccgc cttccggcgc gggtgtaaga gtgacgggat aacgcataat
    10561 ttcgactgtc tctcgcatgt cattaacctt tttaattggc gattaactca aaccgagttg
    10621 tttcaggatt gctttacgca atggttcttt aatctcatcg caggggtgac gcggcatgac
    10681 actgcgcctc ccacgaaacc tgagtttcaa atggttgctg ccattcgcta catcgacgcc
    10741 ctgagattcg agccaacgtc tgaactcgct ttgtttcaca tcgcctccct gccgctgagt
    10801 atatcaacaa agtaaacatt agtgtttacc tccgtgtgta atttgctcga aaatagcact
    10861 aaatcgccgc aacggcagaa tgcaatgcaa aatcaggaag gaagattcca gaatttcttt
    10921 gcgctaattt gcaaaactga ataataacct caagaaacta tcggcatcat agcggcaaaa
    10981 ggtgagaaaa aggtggcggg atgtttacga aggcgttatc ggttgtctta ttaacgtgtg
    11041 cgctgttctc aggacaactc atggcagggc acaaaggaca tgaatttatg tgggtaaaga
    11101 atgtggatca tcagctacgt catgaagcgg acagcgatga attgcgtgcc gtggcggaag
    11161 agtcggcgga aggtttgcgc gagcattttt actggcaaaa atcgcgcaaa ccagaagcgg
    11221 gacaacgttg agtcatttat tgccggatgc ggcgtgacgc cttacccggc caacaatatc
    11281 atgcagattc aataagttgt gagtaaccag aaaactggcg ttttaccctt ttcgcttcgg
    11341 caatgttttc agcagctcat ccggacttac ggaggcgaca acacttcccg gtaacggcat
    11401 tttcagaatg tgatttttaa ttttgccgat gacgtgcatc tcgcatgggc ggcaatcaaa
    11461 ctttagcgtt aatacttcat cgccgttaac cagttgcatt ggcgtcgcct tccagctttt
    11521 gatattgcct ttcgcctgtt tcgggcacag attgaaggca aagcgcaaac aatgcttagt
    11581 gatcatcacc gggacttcgc ccttctcttc atgtgcctca tacgccgcgt caatcagctg
    11641 cacaccgtag cgatgataaa attcacgcgc tttctggttg tatacgttcg cgaggaaact
    11701 cagatgcgtt tgcggataaa ccggcgcagg atcagcaacc ggtttacggc tgccccgctg
    11761 gtaaccggca agacgtgcag catccagcat gtcggcggct tcacggcgga actggtttaa
    11821 cagactgttt ggtacaaaca gcgcacccgg caaattaatt tgcacatcgc gggcgtaata
    11881 gatggtttgc cccagttttg ccagaccatc ctttagattg ttcattgctt tttcggcgtt
    11941 attggcttcg tcgaactgcc catccagcgt atgcgtgatg ctgacaccct cttcactggt
    12001 gagggtcaga atcagttgtt cctgccagcc gcccagttca atgtctaccg ccacccgacg
    12061 ttcgctggag gtttttgtca gtgcctgctg ccagttatga tcaagattac ggtttagtgg
    12121 gtgatgcggg cgaattttgt gcaactctgc tggcatttca ttgggccaga cgcggtactg
    12181 attttctccg gttttctcga ccgtattggc acgaaagccg acgacttcac gtttaatcat
    12241 cacgttcagg ccatcgccat ttgccagtgg ttcggaaacg gcaacatcga gatgatcttt
    12301 cgccactttc aatacttcac ccaccggcag gccgataaat ttcggcgaat cgaacgcgcc
    12361 aatatcgcct ttacgggcat tcacaaaata atctgtgcta ccacggtgga aagtcttttc
    12421 cgtcgatgga acaaagaaat gttcagtacg acctgatgaa gcacgcgtca gatcgacacg
    12481 ttcttcaata atggcatcca gcatctggcg ataatgggcg gtgatattct tcacgtagct
    12541 catatctttg taacgccctt caatcttgaa ggagcgtacg ccagcatcaa tcagcgcgcc
    12601 gaggttggca gtctgatcgt tatctttcat cgacagcaga tgtttttcat aggaaaccac
    12661 ccgcccctga tcgtctttca atgtgtatgg caaacggcac gcctgcgagc aatcgccacg
    12721 gttggcgcta cgccctgttt gcgcatgaga aatgtagcac tgacccgaat aggccacgca
    12781 cagtgcgcca tgaataaaga attcaatggt cgcgtccgtg gcctggtgaa tcgcgcggat
    12841 ctgctcaaga ttcagttctc gcgccagcac aatctgcgtg aagccaacat cagagaggaa
    12901 cttcgctttt tctactgtac gaatgtcgca ctgcgtactg gcgtgcagtt caatcggcgg
    12961 aatatcaagt tccagaatcc ccatatcctg aacaatcagc gcatcgacgc cggtctggta
    13021 gaggtcagta atcagccgtt gcgcaggttc cagctcatca tcatgcaaaa tggtgttaag
    13081 cgtgacgaaa atttttgcac cataacgatg ggcaaacggc accagctcgg caatatcttt
    13141 caggctatta ctggcattat gacgggcacc aaaaccaggg ccgccgatat aaacagcatc
    13201 ggcaccgtgc aaaatagctt cgcgggcaat ggcggcatcg cgtgccggac ttaacagttc
    13261 aagacgatga gaagatacgg tcactttctt atccagtagc tgaaaaatgg cggctatttt
    13321 agccacaagc gcgacgttgc gaaatagttt tcccgcttaa ctgcgcgggt aatggatgag
    13381 ggaatgaaaa tgtacggttt gctcccctcc attacgatag acatgcgtga cgtctgcagc
    13441 aaatcgagcg ccttccccac aattaaggct ctgccattca ccatcaacgc acagatcaag
    13501 ttgtccatca atgacgacaa catgttcgat tccccccttc tcatggggcg tcgattcgct
    13561 tattgcacca ggtgccatct gaatcgagaa gtgttcaaag cagagttgtg gatcataagg
    13621 aaacagtgaa gtgatcacca tcgcctgctg ttgtggatca taaactgaag gcgtagcgga
    13681 ctgcggcgga gaaataaatg ttgaaaatgg cacgttcaac ccggtggcaa ttttccataa
    13741 tgtcgcgacc gtcgggctgg attcattacg ctcaatctgc cctaacatcg ctttagaaac
    13801 gcccgtcgct tctgccagtc gcgaaagact ccagccgcga tgctgacgta gttgtttaag
    13861 tgtggtggat aaaaagcgag cgagattctc catgcgactc ctccggcaaa acggaagttt
    13921 atcacttgtg cgttataacg gacaaatgct acggtacctg tacgttataa cgcacgaggt
    13981 gactatgcgt ctgttttcta ttcctccacc cacgctactg gcggggtttc tggcggtatt
    14041 aattggctac gccagttcag cggcaataat ctggcaagca gcgattgtcg ccggagccac
    14101 cactgcacaa atctctggct ggatgacggc gctggggctg gcaatgggcg tcagtacgct
    14161 gactctgaca ttatggtatc gcgtacctgt tctcaccgca tggtcaacgc ctggcgcggc
    14221 tttgttggtc accggattac agggactaac acttaacgaa gccatcggcg tttttattgt
    14281 caccaacgcg ttaatagtcc tctgcggcat aacgggactc tttgctcgtc tgatgcgcat
    14341 tattccgcac tcgcttgcgg cggcaatgct tgccgggatt ttattacgct ttggtttaca
    14401 ggcgtttgcc agtctggatg atcaatttac gttgtgtggc agtatgttgc tggtatggct
    14461 ggcaaccaag gccgttgcgc cgcgctatgc ggtaattgcc gcgatgatta ttgggatcgt
    14521 catcgttatc gcgcaaggtg acgttgtcac aaatgatgtt gtctttaaac ccgttctccc
    14581 cacttatatt acccctgatt ttgcgtttgc tcacagcctg agcgttgcac tccccctttt
    14641 tctggtgacg atggcatcgc aaaacgcacc gggtatcgca gcaatgaaag ccgccggata
    14701 ttcagctcct gtttcgccat taattgtatt tactggattg ctggcactgg ttttttcccc
    14761 tttcggcgtt tattccgtcg gtattgcggc aatcaccgcg gctatttgtc aaagcccgga
    14821 agcgcatccg gataaagatc aacgttggct ggcagctgcc gttgcaggca ttttttattt
    14881 gctcgcaggt ctttttggta gtgctattac cgggatgatg gctgccctgc ccgtaagttg
    14941 gatccagatg ctggcaggtc tggcgctgtt gagtaccatt ggcggcagtt tgtatcaggc
    15001 gctgcataat gagcgtgagc gcgacgcggc ggtggtggca tttctggtaa cggcaagtgg
    15061 attgacgctg gtcgggattg gttctgcgtt ttggggatta attgccggag gcgtttgtta
    15121 cgtggtgttg aatttaatcg ctgacagaaa ccga
//
LOCUS       DAPOZO010000110        14900 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPOZO010000110 DAPOZO010000000
VERSION     DAPOZO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.110"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(84..2756)
                     /gene="infB"
                     /locus_tag="ROI21_003919"
     CDS             complement(84..2756)
                     /gene="infB"
                     /locus_tag="ROI21_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDX5112788.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(2781..4268)
                     /gene="nusA"
                     /locus_tag="ROI21_003920"
     CDS             complement(2781..4268)
                     /gene="nusA"
                     /locus_tag="ROI21_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDX5112789.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(4296..4748)
                     /gene="rimP"
                     /locus_tag="ROI21_003921"
     CDS             complement(4296..4748)
                     /gene="rimP"
                     /locus_tag="ROI21_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDX5112790.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(4955..5031)
                     /locus_tag="ROI21_003922"
     tRNA            complement(4955..5031)
                     /locus_tag="ROI21_003922"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4995..4997),aa:Met,seq:cat)
     gene            5379..6722
                     /gene="argG"
                     /locus_tag="ROI21_003923"
     CDS             5379..6722
                     /gene="argG"
                     /locus_tag="ROI21_003923"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDX5112791.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(6730..8355)
                     /locus_tag="ROI21_003924"
     CDS             complement(6730..8355)
                     /locus_tag="ROI21_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDX5112792.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQIFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENDIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(8812..8898)
                     /locus_tag="ROI21_003925"
     tRNA            complement(8812..8898)
                     /locus_tag="ROI21_003925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8862..8864),aa:Leu,seq:gag)
     gene            complement(8913..9245)
                     /gene="secG"
                     /locus_tag="ROI21_003926"
     CDS             complement(8913..9245)
                     /gene="secG"
                     /locus_tag="ROI21_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDX5112793.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(9473..10810)
                     /gene="glmM"
                     /locus_tag="ROI21_003927"
     CDS             complement(9473..10810)
                     /gene="glmM"
                     /locus_tag="ROI21_003927"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDX5112794.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(10803..11651)
                     /gene="folP"
                     /locus_tag="ROI21_003928"
     CDS             complement(10803..11651)
                     /gene="folP"
                     /locus_tag="ROI21_003928"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDX5112795.1"
                     /translation="MKLYAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(11741..13675)
                     /gene="ftsH"
                     /locus_tag="ROI21_003929"
     CDS             complement(11741..13675)
                     /gene="ftsH"
                     /locus_tag="ROI21_003929"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDX5112796.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(13775..14404)
                     /gene="rlmE"
                     /locus_tag="ROI21_003930"
     CDS             complement(13775..14404)
                     /gene="rlmE"
                     /locus_tag="ROI21_003930"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDX5112797.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            14530..14823
                     /gene="yhbY"
                     /locus_tag="ROI21_003931"
     CDS             14530..14823
                     /gene="yhbY"
                     /locus_tag="ROI21_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDX5112798.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
BASE COUNT         3644 a         4024 c         3485 g         3747 t
ORIGIN      
        1 tgataagacg cgcgagcgcc gcatcaggca acggctgccg gatgcggcgt aaaagcctta
       61 tccggcctac aacccagaca accttaagcg atggtacgtt ggatttcgat gatttcgaat
      121 acttcgatca catcgccagt gcggacgtcg ttgtagttct taacgccgat accacattcc
      181 ataccgttac ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct
      241 tcgtagataa ccacgttgtc gcgcagaacg cggatcgggt tgtgacgttt aaccacacct
      301 tcggtaacca tacaacctgc gatggcacca aatttcggtg atttgaatac gtcacgaact
      361 tccgccagac cgataatctg ttgtttcagt tccggagaca gcataccgct cattgccgct
      421 ttcacttcgt cgatcaggtt atagatgacg gagtagtaac gcagatccag gctttccgct
      481 tcaatcactt tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg
      541 gacgccgcag ccagggtagc gtcggtttcg gtgataccac ctacgccaga accgatgatc
      601 ttcactttaa cttcgtcagt agacagtttc agcaaggagt cggagatagc ttccacagaa
      661 ccctgtacgt ctgccttcag gacgatattc acttcgtgaa cttcgccttc ggtcatgttg
      721 gcgaacatgt tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta
      781 ccctgacgat agagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca
      841 tcacccgcag ccggaacgcc ggacaggccg aggatttcca ccggaatgga cggacccgct
      901 tccagcactt cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca
      961 cacagaacga tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga
     1021 ccacgacctt tatcgaggaa ggattcgata accgcaccgc tcgccatgcc tttacgtacc
     1081 gctttcagtt ccagaacttc cgcctgcagc aggatagcgt ccagcagctc atcgatacca
     1141 gtacccgctt tcgcagacac atgtacgaac tggctttcac cgccccactc ttccggcagg
     1201 atgccgtact gggagagttc gttcttaacg cgatccggat cggcttctgg tttatcgatc
     1261 ttgttcactg caaccaccac cggaacctgc gccgctttcg cgtgctggat tgcttcgatg
     1321 gtctgcggca ttacgccgtc gtcggcagca acaaccagga ctacgatgtc cgttgcctgc
     1381 gcaccacgag cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc
     1441 atgccgtttt cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg
     1501 ccagaggcca ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg
     1561 tgacccatga tggtcacaac cggcgcgcgc ggttccgccg ctgcccccgt gtcacggtcg
     1621 ctcattaccg cttcttccag ctcgttttca cgacgcagga taactttgtg gcccatctct
     1681 tcagcaacca gctgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc
     1741 agtttcatca tcgctttgat gacctgagag cctttaacag ccatcttgtt cgccagttcg
     1801 ccaacggtga tggtttcgcc gatcacaacg tcacggttaa cggcctgagc aggcttctgg
     1861 aagccttgct gcagcgaaga acctttacgt tttccgcctt taccgccacg tactgctgcg
     1921 cgtgcttctt cacgatcagc ttttgattca gcgtgtttgt tgcctttctt cggacgcgca
     1981 gctttcgcgt tacgaccacg gccacggccg ccttcgactt cacgatcgct ttcgtcttct
     2041 gcctggcgag catgttgaga agtagtgacg tgataatcgc tggaatcttc agtcggttcc
     2101 gcgttatcag tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt
     2161 gcttcttctt cgagtttacg acgcgcttct tcttcggctt tacgcttgag ctctgcagct
     2221 tcctgctcac ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg
     2281 ctcactttgt ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct
     2341 tgttctgcgg cctcacgttc agctttttgt tgcgcctcac gtttggccga ttcttctgcc
     2401 tcacgacggg cttgctcttc cgcttcacgc tgcgcttgct cttccgcagc aaggcgttca
     2461 gcctcttgcg gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat
     2521 ttgctttttc caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc
     2581 aatttgtccg ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt
     2641 gcggacacag agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg
     2701 cgttccacgg aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg
     2761 ttccttcctg ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc
     2821 ataatcagtg ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg
     2881 ccctgttcgg cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa
     2941 tcacgatcta tcccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt
     3001 tcttcctggg cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca
     3061 acggtcggct catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt
     3121 tcttccagcg tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg
     3181 agatatttgg tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg
     3241 tcgtcaacgg tcatcacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc
     3301 tgaccattac ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg
     3361 tgtttatctt catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcacg
     3421 aactgcgccg ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta
     3481 gaaaccgcct gaacacgtgc gccacgcata cctacgcaag cacctaccgg atcgatacgt
     3541 ttatcgttgg ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc cgctgcttta
     3601 atttcaatca cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt
     3661 tccggcttgg aacgagtgac gaacaattgc gcaccacgcg cttccgggcg aacggaatag
     3721 agcacgccac gaacacggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc
     3781 aggatcacgg cttcagcgtt gttgcccaga tccagagaga tgttgtcgcg gtttactttt
     3841 ttcaccacgc cggtgatgat ttcaccttca tgttcacgga actgatcaac caccatcgca
     3901 cgttcggctt cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg
     3961 tcaaaggtaa cagactcaat ctgatcttca acgtaatcgc ccagattcag gctttcatct
     4021 tcataacgtg cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact
     4081 aaccagcgac ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg
     4141 atctcttgtt catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa
     4201 atcttctcgc gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct
     4261 ttgttcatcg cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc
     4321 ttctggatat tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt
     4381 tcaccgtcta ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatgcgg
     4441 agaaccagag tcacctcttc tccgacgaaa cgggcgtagt gttcagccgt gaacagtggg
     4501 cgatcgagac ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca
     4561 tccagcacag cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct
     4621 tcactatcaa tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgccaacc
     4681 agttcaaaac ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat
     4741 gtggacaagc ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc
     4801 agataacaaa aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact
     4861 gcggtctgga gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt
     4921 cacagtatat ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg
     4981 acgaccttcg ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg
     5041 tggcaaattc tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga
     5101 gacgggacgt aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat
     5161 tgctaatcac gtgaatgaat atccggttca ctttcatttg ttgaatactt ttgccttctc
     5221 ctgctctccc ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat
     5281 aaaagatgat taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc
     5341 caggctcatc atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc
     5401 tcccggtagg tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac
     5461 tgctgtggat gcgacaaaag ggagcggttc cttatgcata tactgcaaat ctgggccagc
     5521 cagacgaaga ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg
     5581 cacgtctgat cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg
     5641 gcgcatttca taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg
     5701 ccgtgactgg taccatgctg gttgctgcca tgaaagaaga tggcgtgaat atctggggtg
     5761 acggtagcac ctataaagga aacgatatcg aacgtttcta tcgttacggt ctgctgacca
     5821 atgctgaact gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg
     5881 gccgtcatga gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg
     5941 aaaaagccta ctccacggac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg
     6001 aatacctcaa ctccagcgtc aaaatcgtca atccaattat gggcgtgaag ttttgggatg
     6061 agagcgtgaa aatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtgg
     6121 cgctgaacgg taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg
     6181 gcggtcgtca cggcctgggc atgagcgacc agattgaaaa ccgtatcatc gaagcaaaaa
     6241 gccgcggcat ttatgaagcc cctgggatgg cactgctgca cattgcgtat gaacgtttgt
     6301 tgaccggtat tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg
     6361 gccgtctgct gtaccaggga cgttggtttg actcccaggc gctgatgctg cgggactctc
     6421 tgcaacgctg ggttgccagc cagatcactg gtgaagttac tctggagctg cgccgcggga
     6481 acgattattc aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga
     6541 cgatggaaaa aggcgactcg gtgttctcgc cagatgatcg tatcggtcaa ttgaccatgc
     6601 gtaacctgga tatcaccgat acccgcgaga aacttttcgg ttatgccaaa actggcctcc
     6661 tctcctcctc tgccgcttca ggcgtgccgc aggtggagaa tctggaaaat aaaggccagt
     6721 aattcgacat caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc
     6781 ctttctgagc atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc
     6841 tttatagtgt gcaatcactt cctccagcgt tttcggctgt tccggctttg ttacccccat
     6901 ccacgcatta ataaggtaat tattgtacgc agtggaaaag atatcgtttt ccgttgtacg
     6961 atcaacgcca tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg
     7021 ctggctggcc tccctgcctc catgaaaata gacgttcttc ttcgtagggt cgcgttccag
     7081 gccgtggtcg gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaatctg
     7141 acctagcaaa ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg
     7201 cggttgaaac acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt
     7261 taaatgaaga acaatcagtt tcttttgctg cgtcttttgc tgtaacgctt gcgataaatg
     7321 cggcaacaac aattcatcaa atcctctgac atagactgtt tccatggcgc gcatggcgat
     7381 actggtaact gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg
     7441 aaatcctgct tgattagcca tattaataat gttgtccggg taattatgaa tgtcatgact
     7501 caaaacagaa tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcaccgct
     7561 tattgcttga ttaaacagtt tgatctgttt tctttgtgct tcgacttgcg gtgtcgtaga
     7621 gcgtgtgtat ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac
     7681 gtaggtatca atacctgtat ccctgactga taattgaaaa tatggcaccg tgtttgcaat
     7741 tgagagtaat ctttgatgct ctttcgctgc taaagcaaaa taattgagat tgaaaaacgg
     7801 cgtataggta gcaaatcgcg acgctagtat atatggactg aacgcttttt tattttttgc
     7861 atctttataa gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa
     7921 tattcctgtc acttttttcg tcggcaagga aacatcatat tttattatta ctgccaaaaa
     7981 aagaagggat aaaaaggcaa aagcacatag ataagggata tacatcccca gcattttgac
     8041 aacttcatcc ggatcactct gcagcacact aatcgcaaat ccatcattga aagttgtacc
     8101 aaaggtacac catgaataga ggctaatact catgtctgcc gctgtcagta acacaaatgg
     8161 tgcggcaaca agcaggcgaa gcggaaggga tgatatacgt ttaacaacca ggatggtcag
     8221 aacgaagaac agcaatcgct gaaccatatg cgctccggaa gaggcgacta agttcgtaat
     8281 accaaaaaca ataacacaaa gatacaacaa ccaatgagat ttaaaacttc taacaaattt
     8341 gttgaatact gtcatccaga aatccaagtt cacaaccaat ataaacaatt atcaacaatg
     8401 ttcaatttta tatcagacat gtttttcaac tctgatccat atcagaccga aacactgtta
     8461 gttttttctt tttaaatgat gcggccattg ttctcagtaa ttaaataaca ttatcaatgc
     8521 gtctctaaac attacgcaga acgtagccat cagtagttat actgacagga gcaggatggt
     8581 ttatcgagaa gtaaacatat ccgtattcgt accggaactg gagttgacac aataaagtgc
     8641 caattatgtc agcagaaggg aaaagtttat aactaaggcg taccggcacc atcgtttcaa
     8701 agtaccagct acgagtaaag caactggacg agatacagat acctgacaac cattcctcag
     8761 atcaggacca aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga
     8821 ggacgggact tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac
     8881 caattccacc acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta
     8941 gccggagcag ctggctgagt ttgttcggtt ttggccggcg cactcaggtt ttcccattcg
     9001 ctacctttat tggttttgtt gctattgatg ttacccagta ccagactgat gatgaagaat
     9061 aacgttgcca gcagcgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac
     9121 agcgtagcgg aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc
     9181 agcatgatca gaccaacaag gccaattgcc acaataagga aaactaccaa aagagcttcg
     9241 tacataatca acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg
     9301 ggagttttta atctttccca ctgaagcggg tgtgaatact aaccaaagcg aatgaccttc
     9361 gcaagggcaa ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt
     9421 cgccgagaaa aaaggcgaca atcgccgcct ttttagccag tcatctaacg ctttaaacgg
     9481 cttttactgc atcggcgata cggtgtgcaa attcagttac ctgcgcttcg tcttcacctt
     9541 ctaccatcac gcgaattaac ggctcggtgc cagatttacg cagcaaaacg cgtccacggc
     9601 tacccagcgc cgcttcaact tctgcggtca cagctttgac tgattcatgc tccagcggat
     9661 cgccgctacc tgcggtgtaa cgcacgttaa ccagaatctg cgggaacatt ttcatgccgc
     9721 tgcaaaggtc gtgcaggctc atatgattac gtgccatcgc cgccagcacc tgcaagccag
     9781 caacgatgcc gtcaccggta gtggttttat ccagcaggat cacatgaccg gagttctctg
     9841 caccgatacg ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg
     9901 cgcgcgcaaa tggaatacct aactgtttca gcgccagttc aagccccatg ttgctcatca
     9961 atgtacccac cgcgccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat
    10021 acatgatctg atcgccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc
    10081 cgtcgccgtc gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg
    10141 cgcgaacgtc ggtagccccc acttcggcat tgatgtttac accgttcggc tcacaaccga
    10201 tagcgataac gttcgccccc agttcgcgca gcacgttcgg cgcgatgtga taagtcgcac
    10261 cgtttgcgca atccaccaca atcttcagtt cactgaggct aagttcgttc gggaacgtgg
    10321 ctttgcaaaa ctcgatatag cgacccgcag catcaacgat acggctggct ttacccagtt
    10381 ctgccgaatc aacacagctg atctcttttt ccatttccgc ttcgatggct tcttcaactg
    10441 catccggcag cttagtaccg tcaatagaaa agaatttaat gccgttatcg tagaatgggt
    10501 tatgcgatgc agaaattaca atcccggcct ctgcgcggaa ggtacgcgtc agataagcca
    10561 cggcaggtgt aggcatcggg cctgtaaaga gtgcggaaag gcccgcagcc gccagacccg
    10621 cttccagtgc tgactccagc atatagccag aaatacgcgt gtctttacca ataatgatct
    10681 tacgggagcc gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat
    10741 caggggtgat cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac
    10801 gattactcat agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct
    10861 tctacggttt ctttgacgtc atgaacacga atgatatgcg cgccttgcat agcggcaatg
    10921 accgcacaag ccagactacc gctcagacgc tcagatggcc ccacgttcag caattgccca
    10981 atcatcgatt ttcgtgacat acccaccaac agcggcaggt tgaaatgatg aaattcagcc
    11041 aggcgcgcca gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg
    11101 agcaacaatt tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag
    11161 tagcgattca cttctgcaaa gacatcgtca tacttcgggg cttcctgcat ggtttttgga
    11221 tttccctgca tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg
    11281 ccaggttcgg aaagggagcg gatatcgtta ataatgtgag cgccaacttt cgctgactca
    11341 cggatgactt ctggtttgga tgtatctacc gagatccaga cttcgaagcg ttgagcaatt
    11401 gcctcaacca caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct
    11461 gggcgcgtgg actcaccacc aacgtcaatg atcgtcgcgc cagcgttgat catcagattc
    11521 gcatgtttca ccgcatctat cagcgagtta tgcgtgccgc catccgaaaa ggaatcaggc
    11581 gtgacgttga ggatccccat tacgtgagga tggctaaggt ccagtgaagt gccctgggca
    11641 tagagtttca tggtgttatc cctagtatga attgataaga aaaaaccccg gagcacgccc
    11701 cggggttttc ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct
    11761 ctgacatggt gttacccggg ttcggcgtac gcggttcatc aaccggacgt ggagcctttg
    11821 gactaccatt gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac
    11881 gtacatcgcg acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca
    11941 tgagagcatc tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac
    12001 gattatagtt acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat
    12061 cggacatatg tttcgctttc gctacgctac ggccgaggaa cacttcacct tcttcttccg
    12121 cgtacagcag tggacccaat ttctcagaga agccccactg agtcaccatg ttacgtgcca
    12181 ggttggtcgc aactttaata tcgttggacg caccggtaga aacatgttcc ggcccgtaga
    12241 tgatctcttc tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac
    12301 ggctggcgct gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc
    12361 gtgggataat cgtcactttg tgcaccggat cgtgttccgg caccaggcga ccgataatcg
    12421 cgtgacccgc ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc accatggagc
    12481 gacgttccgc acccatcatg attttgtctt tcgctttctc gaactcaacc atcgacacaa
    12541 cgcgtttgtt gccacgagca gcgaacagtg ccgcttcgtt caccaggttc gccaggtcag
    12601 caccggagaa acctggagta ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg
    12661 gtacacgacg catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccaa
    12721 ccacaacctg acggtcgaaa cggccaggac gcagcagcgc cgggtcgaga acgtccggac
    12781 ggttagttgc ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca
    12841 gcatctggtt cagagtctgt tcacgttcat cgtgaccacc gcccaggcct gcgccacgct
    12901 ggcggcctac ggcgtcgatt tcatcgataa agatgataca cggtgccgct ttcttcgcct
    12961 gttcgaacat gtcacgaaca cgggatgcac ccacaccgac gaacatttcg acgaagtcag
    13021 aaccggagat agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg
    13081 ttttaccggt acccggagga ccgaccatca gaacgccttt cgggatctta ccgccgagtt
    13141 tctggaagcg gctcggctcg cgcagatact caaccagttc agcaacttct tcttttgctt
    13201 cgtcgcagcc cgcaacgtca gcaaaggtcg ttttgatctg atcttcagtc agcatgcgcg
    13261 ctttgctctt accaaacgac atggcacctt tgccaccgcc gccttgcatt tgacgcatga
    13321 agaagatcca gacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca
    13381 gcaggcttgg ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat
    13441 ccagtaattt cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg
    13501 taacgttgat ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt
    13561 gtaggaaggt agagtaatcc accttacggc cattagactc gctgggccca aagctctgga
    13621 atactgacat cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac
    13681 tcaagggatt aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc
    13741 agcatctttc aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa
    13801 tatacacttc ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa
    13861 acagggagcg aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta
    13921 caaaactgcc acctggcgct aatacatcac gacacatttc tagcgccagt tccaccagat
    13981 acatggcacg ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca
    14041 tgacaacctg gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat
    14101 cacgaaaatc gccctgaaga aagtccacac caacgatagg atccataggt aaaagatcgc
    14161 aagcgatgat gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg
    14221 gagcagcacc aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta
    14281 tttcatcaag tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat
    14341 atttatcgct aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg
    14401 tcatttaact ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc
    14461 tatttggtga tatatgggag atggcggtag aatgacccgt tttcaatccc aacgtaagca
    14521 aaaaatacga tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg
    14581 ctcaagccag ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt
    14641 gaacaagcgt tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa
    14701 actaaaacct tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc
    14761 atcggtaaaa cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc
    14821 taagattatc ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag
    14881 gagagcaaaa tgccacgctc
//
LOCUS       DAPOZO010000111        14878 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPOZO010000111 DAPOZO010000000
VERSION     DAPOZO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.111"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(312..530)
                     /gene="infA"
                     /locus_tag="ROI21_003932"
     CDS             complement(312..530)
                     /gene="infA"
                     /locus_tag="ROI21_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDX5112799.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(815..1519)
                     /gene="aat"
                     /locus_tag="ROI21_003933"
     CDS             complement(815..1519)
                     /gene="aat"
                     /locus_tag="ROI21_003933"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDX5112800.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1561..3282)
                     /gene="cydC"
                     /locus_tag="ROI21_003934"
     CDS             complement(1561..3282)
                     /gene="cydC"
                     /locus_tag="ROI21_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDX5112801.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTILGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAKLLARQGRY
                     YQFKQGL"
     gene            complement(3283..5049)
                     /gene="cydD"
                     /locus_tag="ROI21_003935"
     CDS             complement(3283..5049)
                     /gene="cydD"
                     /locus_tag="ROI21_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDX5112802.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEELFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5172..6137)
                     /gene="trxB"
                     /locus_tag="ROI21_003936"
     CDS             complement(5172..6137)
                     /gene="trxB"
                     /locus_tag="ROI21_003936"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDX5112803.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFIAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6682..7176
                     /gene="lrp"
                     /locus_tag="ROI21_003937"
     CDS             6682..7176
                     /gene="lrp"
                     /locus_tag="ROI21_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDX5112804.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7311..11378
                     /gene="ftsK"
                     /locus_tag="ROI21_003938"
     CDS             7311..11378
                     /gene="ftsK"
                     /locus_tag="ROI21_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDX5112805.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQPTVAWQPVPGPQTGEPVIAPAPEGYPQQLQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAAEQSAQQPYYAPA
                     PEQSAAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDHYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPANAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQQQYQQPQQPVAQQPQYQQPQQPVAPQPHDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11537..12148
                     /gene="lolA"
                     /locus_tag="ROI21_003939"
     CDS             11537..12148
                     /gene="lolA"
                     /locus_tag="ROI21_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDX5112806.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGTVDAAKFTFTPPQGVTVDDQRK"
     gene            12159..13502
                     /gene="rarA"
                     /locus_tag="ROI21_003940"
                     /gene_synonym="mgsA"
     CDS             12159..13502
                     /gene="rarA"
                     /locus_tag="ROI21_003940"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDX5112807.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTCYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13593..>14878
                     /gene="serS"
                     /locus_tag="ROI21_003941"
     CDS             13593..>14878
                     /gene="serS"
                     /locus_tag="ROI21_003941"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDX5112808.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEY"
BASE COUNT         3752 a         3824 c         3927 g         3375 t
ORIGIN      
        1 tacgttgccg tatacacact ttccaggcgt gctccttcag ccactcggac acctcaccaa
       61 attgttttgc taccaaacct catgggtggc aacggggcgc tactataggg agttggagta
      121 aaacggtcaa gaagaatttt aatgataatt attgtttgct catactgtaa acaagttgtg
      181 cagtatatct acatcgagac aagttatgga cttatacttc caaagtactt catacatatc
      241 acaaaataaa aaggccggtt aaaccgacct tttactcgtt ctttctcttc gcccatcagg
      301 cggtaaaaca atcagcgact acggaagaca atgcggcctt tgctcaggtc gtacggggtc
      361 agttcaacag tcactttgtc gcccgtcagg atgcggatgt agtttttgcg cattttaccg
      421 gagatgtgtg cagtaaccac gtgaccgttt tctaactcta cgcggaacat ggtattaggc
      481 aacgtttcaa gaacggtacc ttgcatttca atattgtctt ctttggccat ctaatcctct
      541 ggggtatcac taccgtaatt tgaaccggca agataatgcc gaagttctgt aaataagtaa
      601 agatttgcgc gctaaatcgc aacaaacagg ttcggcacat tactccgaaa acacacggct
      661 aagccgcacc aaaagcgcaa cgtataaggg agcggtgaga taaacgatgg gcgttacctg
      721 acgcgaaaaa ttccttatcg gcagcggggt aatgagcgta accaactctg cgaccgcaat
      781 tataacactc tggggagaaa tgtgccgaaa acattcattc ttgtggtgaa aacaagcacc
      841 gtggtaccca gaaattattc ggcaatcgtc cgaggcgcat ttgattgaga taattgaggt
      901 aatcccggcg gggaatttcg caggcaccaa gcgacgctgt gtgatcgtta aggacctggc
      961 agtcgataag tttaccgcca tgaccaataa attcctcaca gaataccaga agcgccgttt
     1021 tagacgcatt ttccatccgg ctgaacatgg actcgccaca aaatagcgtt ccctgggcca
     1081 cgccgtacat accgccgaca agctcatctt cacgccagac ttcaatagag tgggcatgac
     1141 cgagttcgtg aaggcgatgg taggcttcga ccacgccacg cgtgatccag gttccttctt
     1201 cgcgatcgct ggcacagcct tcaatgacct gaccaaaagc gtaattcatc gtgactcgat
     1261 agggcgagcg tttatgaaat cgcttcatac tacggctgat atgcagtgat tcaggccaaa
     1321 gcaccgcgcg aggatcgggc gaccaccaga ggatggggtc acctggagaa aaccacggaa
     1381 aaataccacg ctggtaagcc attaacaggc gcgcagggct aagatcgccc cccagtgcca
     1441 gcaggccgtt aggctcacgt aatgcgcctt ccggggaagg gaaggctatt gaatggcgag
     1501 aaagctgaac caggcgcatg accgcaaaac tccacgcaag tcggatcgtt caataatagc
     1561 ttacaaaccc tgcttaaact ggtaataacg cccctgtctg gcaagcagtt ttgcgtgagt
     1621 accttgctca ataatttgcc cgttgtccat cactattatt tgttggaaac gagagagtcc
     1681 acgaagtcga tgggtgacca ttaacaccgt tttctcacgc atcatttctg caagcaattc
     1741 taggatctga ctttcggttg tggcatctaa gccttctgta ggttcatcca gcaataccag
     1801 cggcgcatca tgtaacagcg cacgggcgat agccagacgg cgtagttcac caccggagag
     1861 ctggcgtccg ccttcaccca accaactgtt gagacctgca tcctcgagca gcttctccag
     1921 accaacgcga cgtaagatct ccgccagagc ctcatcacta ctgccaggcg aggcgagtaa
     1981 cagattatca cgcagcgtgg cgctaaacag atgcactcgc tgtggcacaa cgctgatggt
     2041 ctgtcgtaga gtcgcttcat tcaggctggc tatggggcta tcgttaagca aaatctcgcc
     2101 ctgttgcggg tcccatgcgc gggtcagcag ttgtaacagt gttgatttgc cgcatccggt
     2161 tcgcccgaga atcgctatat gttctccggc gtttacctga agagaaatcc ctttaagtgc
     2221 ctgttgggat tgctccggat aagtgaactg aacatcccgt aacgtcagcg aaacgcgatc
     2281 ggcaacacga gtttgggtat cagggaaggt gacctccggt ttttgatccg ttaagtcagt
     2341 gatccgtacg gcagaggcaa tgacttgccc cagatgctga aatgcacccg ttactggtgc
     2401 cagtgcttca aacgcggcta acgcgcagaa gacaaacagg gcaattaacg cgccgggttg
     2461 agcattgccg ccaacgccgc cagacgccat ccacagcatc aggatcaccg ctaacgcgcc
     2521 aatgagcagc attatcgctt gcgacaatgc ggtgagttca gattgacgtc gttgcgcttc
     2581 cagccactga atttcggtat tctctaactg cgtgcgataa cgatcgctgg cacccaaaat
     2641 ggtcagctca gcttgccctt gcagccaggc cgtcagttgt tggcgatact gcccgcgaag
     2701 atgagtcaga ttttgcccgg tgctttttcc cgcacgataa aacagcggtg gcatcaggaa
     2761 aagcgtcagt aacataatgc cgcccagcgt aaaggcgagg gtgaaatcaa ggaaacttaa
     2821 cccgattgtc accaccataa tcaccacaaa agcgcccacc agcggcgaga taacgcgcag
     2881 gtaaagatga tcgagcgtat caacatccgc caccacgcgg ttgagcaatt cgccctgacg
     2941 atagcgcgcc agtccggcag gggagagggg cagcaatttg ctgaaggtat aaatgcgcag
     3001 atgctgcaac acgcggaaag tcgcgtcgtg acttaccaga cgttcaaaat agcgcccggc
     3061 agtacgggtg attgctgcac cacgcacgcc cgcagcgggt agcatatagt tgaagctgta
     3121 cagtccggca acccccgcta ccgctgaggc cgagaggaac cagccggaaa gtgtcaacag
     3181 accgatactg gcgagcagcg tgacaattgc cagcacaata ccaagactta acatccattt
     3241 atgacgttta tacagtgcca gatagggtag caaagcgcgc atttaaatct cctcctgacg
     3301 atgggccagt aatgtggcga atgggccacc agccacactt aattccgcgt aacgtccttg
     3361 ctcaataatc tgaccatcct gcattaccca aatgacatcc cagtcagcaa gatcttctaa
     3421 ctggtgggtg accattaacg ttgtctggcg cagagaggcg gcattaagcg cctccattac
     3481 gcgctgttca ctgtgagcat caaggctggc agcgggttca tccaacagta atagcgaaca
     3541 gggatttagt aatgctcggg ccaccgccac gcgctgtgcc tgccccacgg aaaggcgggc
     3601 agcctggtcg ccaacaggcg tatcaacgcc ttgtggcagg agcggtagaa actcactgac
     3661 ccaggcgtta tccagcgctg tttgtaactc gtgttcgctg gcatcaggtc gtgccagtag
     3721 tacgttatcc cgtagtgttg ctgctggtaa ttgtgggttt tgcccaaccc aggagagatg
     3781 tttacgccat gattccggtg ataaatcgcg taattctatc ccgttgattc gtagcgatcc
     3841 ctgatatgag agaaaaccag aaagcgcgtt cagcagtgag cttttacctg aaccgctgcg
     3901 accaaccaac acggcacgtt ggcctgctgg caaagtaaag ttcagcggtc cggccagcgt
     3961 tttaccttct ggtgatgtaa taaacagctc ctcggcttca atggtcaccg gatcggtcga
     4021 tgctaattcc gcctcaccac gctgcggatg ggcaagcggg gtttccataa acgttttcaa
     4081 actgtcagct gcaccaacag cctgggcttt agcatgataa aacgtaccga gatcgcgtaa
     4141 tggctggaaa aactccggcg caaggatcag ggccagaaaa cccgcagcca gcgtcacgcc
     4201 agtatcgtag tgaccaaaat ccagctcgcc gagataggaa aaaccaaagt agaccgccac
     4261 cagagcaatt gacagcgagg taaaaaattc gagaatgccg gaggataaaa acgccagccg
     4321 tagcacttcc attgtccgtt ggcggaaatc ttccgaagca gaacgaatac tttcaatttc
     4381 agcttcacca cgaccaaaaa tacgcaatgt ttccatgccg cgcaggcgat cgagaaaatg
     4441 cccacttaag cgagcaagag cgagaaagtt acgtcggtta gcatcggcag cccccattcc
     4501 aaccagcgcc ataaacaacg gaattaacgg agcagtgccc agcagaatca gcgccgcagc
     4561 ccagttagag gggaagatag ccaccacaat cagcaacggc accgacactg ccagcgccat
     4621 ttgcggcagg tagcgtgcat agtaatcatg catatcgtca atttgctcga gtaccagcgt
     4681 cgcccagctc cccgccggtt taccctgaat ccacgctggc cctgcttgtt gcagacggtc
     4741 gagaacctga cggcggatgg caaagcggat atgctgcccg gcgtgataac ccacccgttc
     4801 gcgtaaccag accacccatg cgcgcagtac aaaggtcaga accagtaacg taaagggaag
     4861 cagcagggct tcacggggaa tattctccat aatcatatgt tgcagaattc gcgccatgaa
     4921 ccaggcctgg gcaatgatca atatgccgct cacaaagccc agcagacgag aaatattcag
     4981 ccaacgttgg gagatgacgc tttgctgttt taaccagcgg gttaactctt tttgacggga
     5041 tttattcatt gcacgcttag caggtgagtt atcagaatta tttgcagagc aatgttacaa
     5101 cgaggaaaaa ataaaggcga cccatagtcg catggtgtcg ccttctttac ttttgttact
     5161 gatttgtaaa attattttgc gtcagctaaa ccatcgaggt agcgttccgc atcaagtgct
     5221 gccatgcagc ctgtaccagc agatgtaata gcctggcgat aaatgtgatc catcacgtcg
     5281 cctgcggcaa agacgccagg gatgctggtc tgggtggcat taccatgaat acccgactgt
     5341 actttgatgt agccgttttc cagttccagc tgcccttcga aaatagccgt attcgggctg
     5401 tgaccgatag caataaacag accggcaacg tcgagtgact cgatgttatc gctgttttgc
     5461 gtatcgcgca gacgaacgcc agtgacgccc atctgatcgc cggtcacctc ttccagcgta
     5521 cggttggtgt gtagaatgat gttgccgttc tccactttat ccatcagacg cttaataagg
     5581 attttttccg cgcggaaacc gtcacggcgg tgaatcagat gcacttccga agcgatgtta
     5641 gacagataca gcgcctcttc aaccgcggta ttgccgccgc cgatgaccgc aactttctgg
     5701 ttgcgataga agaaaccgtc gcaggtagca caagcagaaa ctccacggcc tttaaaggct
     5761 tcttcagatg gcaggccgag atagcgtgca gaagctccgg tggcaataat cagcgcgtcg
     5821 caagtgtatt cgccgctatc gccagtcaga cggaacggac gattctgcaa atcaactttg
     5881 ttgatgtgat caaagatgat ttcagtttca aatttggtgg catgttcgtg catgcgctcc
     5941 attaataacg gaccggtcag atcgtttgga tcgccaggcc agttttccac ttccgtggtg
     6001 gtggtcagtt ggccgccttt ttccatgccg gtaatcagca caggttgcag gttggcgcgc
     6061 gccgcgtaga cagcagcggt atatcccgcc gggcctgaac ccaggataag cagtttactg
     6121 tgtttggtcg tgcccatgag atccccatag ttgttggcag acaatgggca ggattgtagg
     6181 gaatttacag acgtaaaaaa agagtatgac gattttgtta acaatttgtg caatcggcag
     6241 catcgataag caggtcaaat tctcccgtca ttatcacctc tgctacttaa atttcccgct
     6301 ttataagccg attaaatgat gaataaacgc ccctgttaat gaatatctgg catgttgtac
     6361 taaaaatcga tgttttgctt tgacaatccc ctggtgtttt gcgaaaacat tcgaggaaga
     6421 aaaaaaacag tattcttata tgcgcataac catgcatgta aataccatgt ttaccgtgct
     6481 agtgaaatct acgtatggcg tggacagacg ccattcgtga tgtcgatagc tgccacaagg
     6541 caacggtctt ctcaccgtag acccaggcat tgcgcgccgt gaatcttcat gatttcggtc
     6601 tatcgtgacg ggtagcgact ctgaacagtg atgtttcagg gtcagacagg agtagggaag
     6661 gaatacagag agacaataat aatggtagat agcaagaagc gccctggcaa agatctcgac
     6721 cgtatcgatc gtaacattct taatgagttg caaaaggatg ggcgtatttc taacgtcgag
     6781 ctttctaaac gtgtgggact ttccccaacg ccgtgccttg agcgtgtgcg tcggctggaa
     6841 agacaagggt ttattcaggg ctatacggcg ctgctgaacc cccattatct ggatgcatca
     6901 cttctggtat tcgttgagat tactctgaat cgtggcgcac cggatgtgtt tgaacaattc
     6961 aataccgctg tacaaaaact tgaagaaatt caggagtgtc atttagtatc cggtgatttc
     7021 gactacctgt tgaaaacacg cgtgccggat atgtcagctt accgtaagtt gctgggggaa
     7081 accctgctgc gtctgcctgg cgtcaatgac acacggacat acgttgttat ggaagaagtc
     7141 aagcagagta atcgtctggt tattaagacg cgctaacacg gaacaggtgc aaaatcggcg
     7201 tattttgatt acactcctgt taatccatac agcaacagta ctggggtaac ctggtactgt
     7261 tgtccgtttt agcatcgggc aggaaaagcc tgtaacctgg agagcctttc ttgagccagg
     7321 aatacactga agacaaagaa gtcacattga caaagttaag tagcggccgc cgccttctgg
     7381 aggcgttgct gatccttatt gtcctgtttg ccgtctggtt gatggctgcc ttactaagct
     7441 ttaacccttc ggaccccagc tggtcgcaaa cggcctggca tgaacctatc cataatttag
     7501 gtgggatgcc cggtgcatgg ctggcagata cgctgttctt tatttttggc gtgatggctt
     7561 acaccattcc cgtcattatt gtcggcggtt gttggtttgc ctggcgtcat cagtccagcg
     7621 acgaatacat tgattatttt gccgtttcgc tacgcatcat tggcgttttg gcgctcatcc
     7681 ttacctcctg tggtctggcg gcaatcaacg ctgacgatat ctggtatttt gcctccggtg
     7741 gcgtcattgg cagcttacta agcactacgc tacaaccact gctacacagt agcgggggaa
     7801 ctattgcgct gctctgcgtt tgggctgcgg gtctgacgct gttcaccggt tggtcatggg
     7861 tgaccattgc tgaaaaactc ggcggctgga ttttaaatat tctcaccttc gccagtaatc
     7921 gtacccgtcg cgatgatacc tgggtcgatg aagatgagta tgaagacgac gaagagtatg
     7981 aagatgaaaa tcacggcaaa cagcatgaat cacgccgtgc ccgtattctt cgcggcgcgc
     8041 tagcgcgtcg taaacggttg gcggaaaaat tcattaatcc gatggggcgg caaacagatg
     8101 ctgcgttgtt ctccggtaag cggatggatg atgaagaaga gattacctac actgcacgcg
     8161 gtgtggctgc cgaccctgac gacgtcctct tttcgggcaa tcgtgcaacg cagccagaat
     8221 atgacgaata cgatccatta ttaaacggtg cgccaattac cgaacctgtt gctgtagcag
     8281 ctgctgctac cactgcgaca caaagctggg ctgcgccggt tgaacctgtg actcagacgc
     8341 cgcctgttgc ctctgttgat gttccacctg cacaacctac agtagcctgg cagcctgtac
     8401 cggggccaca aacgggagag ccggttattg ctcctgcacc ggaaggttat ccacagcagc
     8461 tacaatatgc gcagcctgca gtgcaatata atgagccgct gcaacaacct gtacagccac
     8521 agcagccgta ttatgcgcca gcagcggaac aacctgtgca acaaccttac tacgctcctg
     8581 cggcggaaca acctgttcag cagccgtatt acgcaactgc ggcggaacag tctgcgcaac
     8641 agccgtatta cgcccctgcg ccagaacaat cggcggcagg taacgcctgg caagccgaag
     8701 agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact tatcagcagc
     8761 cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag cctgttgtgg
     8821 agcctgaacc cgttgtagaa gaaacaaaac ccgcgcgtcc gccgctttac tattttgaag
     8881 aagtggaaga gaagcgtgcc cgtgaacgtg aacaacttgc ggcctggtat caaccgattc
     8941 ctgaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct tctgttgcag
     9001 cagtacctcc agtggaagcc gctgccgctg tttcccctct ggcatctggc gtgaaaaaag
     9061 cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt ctggcaaata
     9121 gcgctggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca cgaccgaaac
     9181 gtatccgcgt gccaactcgt cgtgaactgg cgtcttacgg tattaagctg ccctcacagc
     9241 gtgcggcaga agaaaaagcc cgtgaagccc agcgcaatca gtacgattcc ggcgatcatt
     9301 ataacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag ttcgcccaga
     9361 cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgcgaac gcagaagatg
     9421 cagatgctgc ggcagaggct gaactggctc gtcagtttgc tcaaactcaa caacaacgtt
     9481 attccggcga acaaccggct ggggcgaatc cgttcacgct ggatgatttt gaattttcgc
     9541 caatgaaagc gttgctggat gatggtccac acgaaccgct gtttacgcca attgttgaac
     9601 ctgtacagca gccgcaacaa ccgattgcac cgcagcaaca gtatcagcag ccgcagcaac
     9661 cagttgcgcc gcagccgcag tatcagcagc cgcaacagcc ggttgcaccg cagcagcaat
     9721 atcagcagcc acaacaaccg gttgcgccgc agcagcaata tcagcagccg caacagccgg
     9781 ttgcacagca gccgcaatat cagcagccgc aacagccagt tgcgccgcag ccgcatgata
     9841 ccctgcttca tccgctgttg atgcgcaatg gtgacagccg tccgttgcat aaaccgacga
     9901 cgccgctgcc ttctctggat ttgctgacac cgccgccgag cgaagtggag ccggtagata
     9961 cctttgcgct tgaacaaatg gcgcgtctgg tggaagcgcg tctggctgat ttccgtatta
    10021 aagccgatgt cgtcaattac tctccggggc cggttatcac tcgctttgaa cttaacctgg
    10081 caccgggcgt aaaagcggcg cgcatttcta acttgtcacg ggaccttgcc cgttcacttt
    10141 cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa accctatgta ggtctggagt
    10201 taccgaataa aaaacgacaa accgtttatc tgcgcgaagt attggataac gccaaattcc
    10261 gcgataatcc gtcgccatta accgtggtgc tgggtaaaga tatcgccggt gagccggtgg
    10321 ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg gactaccggt tccggtaagt
    10381 ctgtcggggt gaacgcgatg atcctgagca tgctttataa agcacagcca gaagatgtgc
    10441 gtttcatcat gatcgacccg aaaatgctgg agctttcggt ttatgaaggc attccgcatc
    10501 tgttaacgga agtcgttacc gacatgaaag atgctgccaa cgctttgcgc tggtgcgtaa
    10561 atgaaatgga acgtcgttat aagctgatgt ccgccctagg tgtgcgtaat ctggcgggtt
    10621 ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc gattccagac ccgtactgga
    10681 agccgggtga cagtatggat gcccagcatc cggtgctgaa aaaagaaccg tacattgtgg
    10741 tgttggtgga cgaatttgcc gacctgatga tgacggtagg taaaaaagtg gaagagctta
    10801 tagcacgtct ggcgcaaaaa gcccgtgccg cgggtatcca cctcgtactg gcaacgcagc
    10861 gtccatcggt tgatgttatt actggtctga ttaaagcgaa tattccgacc cgtatcgcct
    10921 ttaccgtatc cagtaagatt gactcacgta ccattcttga tcaggctggt gcggaatcac
    10981 tgctgggtat gggggatatg ctctactctg ggccgaactc cacgttgccg gtacgtgtcc
    11041 atggtgcttt tgttcgcgat caggaagttc atgccgtggt gcaggactgg aaagcgcgtg
    11101 gtcgcccaca gtatgttgat ggcatcacct ccgacagcga aagcgaaggt ggcgcgggcg
    11161 gtttcgatgg cgctgaagag ctggatccgt tgttcgatca ggcggtgcag tttgtcactg
    11221 aaaaacgcaa agcatcaatt tctggcgtac agcgtcagtt ccgcattggt tataaccgtg
    11281 cagcgcgtat tatcgaacag atggaagcgc aggggattgt cagcgaacag gggcacaacg
    11341 gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt aatgcatcgt aggccggata
    11401 aggctcggta gcgtcgcatc cggcactcta acaactgaaa attcagtatt ttcttctttc
    11461 ctcaagctga ttattagcct ggaatagaga gtagagggaa ctcccgatcg ggagtgacgt
    11521 aatttgagga ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt
    11581 agcaagcagc gtttgggctg atgccgcaag cgatcttaaa agccgcctgg ataaagtcag
    11641 cagcttccac gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga
    11701 aggccagggc gatctgtggg tgaaacgtcc aaacttattc aactggcata tgactcaacc
    11761 tgatgaaagc attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga
    11821 gcaagctacg gcaacctggc tgaaagatgc caccggtaat acgccgttta tgctgattgc
    11881 ccgcaaccag tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt
    11941 cctgacgccg aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga
    12001 tggcacaatc catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcagct
    12061 gaaatcccag caaaatggga ctgtggatgc agcgaaattt accttcaccc cgccgcaagg
    12121 cgtaacggta gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt
    12181 tttcggataa tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt
    12241 atatcggcca gcaacatttg ttggctgcgg ggaagccgtt gccgcgtgct atcgaagccg
    12301 ggcatttgca ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg
    12361 aagtgattgc ccgctatgcg aacgctgatg tggaacgtat ttctgccgtt acttctggcg
    12421 tgaaagagat tcgcgaggcg atcgagcgcg ctcggcaaaa ccgcaatgct ggtcgccgca
    12481 ctattctttt tgttgacgaa gttcaccgat tcaacaaaag ccagcaggat gcatttctgc
    12541 cacatattga agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg
    12601 aacttaactc ggcactgctt tcccgtgccc gtgtctatct gctgaaatcc ctgagtacag
    12661 aggatattga gcaggtgctt actcaggcga tggaagacaa aacccgtggc tatggtggtc
    12721 aggatatagt tctgccagat gaaacgcgac gcgccattgc tgaactggtg aatggcgacg
    12781 cgcgccgggc gttaaatacg ctggaaatga tggcggatat ggccgaagtc gatgatagcg
    12841 gtaagcgggt cctgaagcct gaattactga ccgaaatcgc cggtgaacgt agcgcccgct
    12901 ttgataacaa aggcgatcgc ttttacgatc tcatttccgc actgcataag tcggtacgtg
    12961 gtagcgcacc cgatgcggcg ctgtactggt atgcgcgaat tattactgcc ggtggcgatc
    13021 cgttatatgt cgcgcgtcgc tgtctggcga tagcgtcgga agatgtgggt aatgctgatc
    13081 cacgagcgat gcaggtggca attgcggcct gggattgctt tacccgagtt ggcccggcgg
    13141 aaggagaacg cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg
    13201 ctgtctacac tgcgtttaaa gccgcgctgg cggatgctcg cgaacgtccg gattatgacg
    13261 tgccggttca tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg
    13321 aatatcgtta cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc
    13381 cggaaatagc acaaacatgc tattatttcc cgacaaacag gggccttgaa ggcaagattg
    13441 gcgaaaagct cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt
    13501 aatgttatcg ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat
    13561 ctcccattta attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg
    13621 agccagacgc agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc
    13681 tgggcgctct tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg
    13741 agcgtaactc ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc
    13801 ctttacgtct ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg
    13861 atgctttaca ggctgaaatt cgcgatatcg cgctaaccat ccctaacctg cctgcagatg
    13921 aagtgccggt aggtaaagac gaaaatgaca acgttgaagt cagccgctgg ggcaccccgc
    13981 gtgagtttga ctttgaagtt cgtgaccatg tgacgctggg tgaaatgcac tctggcctcg
    14041 actttgcagc cgcagttaag ttgaccggtt cccgctttgt ggtaatgaaa gggcagattg
    14101 ctcgcatgca ccgcgcacta tcgcagttta tgctggatct gcataccgaa cagcatggct
    14161 acagtgagaa ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc
    14221 tgccgaaatt tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca
    14281 gtaactatgc gctgatccca acggcagaag ttccgctgac caacctggtg cgcggtgaaa
    14341 tcatcgatga agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg
    14401 aagccggttc atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag
    14461 ttgaaatggt gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgactg
    14521 gtcatgcaga aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc atcctttgta
    14581 ccggcgacat gggctttggt gcttgcaaaa cttacgacct ggaagtatgg atcccggcac
    14641 agaacaccta ccgtgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta
    14701 tgcaggcacg ttgccgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg
    14761 gttctggtct ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat cagcaggctg
    14821 atggtcgtat tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatat
//
LOCUS       DAPOZO010000112        14827 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPOZO010000112 DAPOZO010000000
VERSION     DAPOZO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.112"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(60..944)
                     /gene="xapR"
                     /locus_tag="ROI21_003942"
     CDS             complement(60..944)
                     /gene="xapR"
                     /locus_tag="ROI21_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HDX5112809.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVDVLFREKMPAMQMALLERRELDAGIWRMATEPPA
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(985..1143)
                     /locus_tag="ROI21_003943"
     CDS             complement(985..1143)
                     /locus_tag="ROI21_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HDX5112810.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(1196..2452)
                     /gene="xapB"
                     /locus_tag="ROI21_003944"
     CDS             complement(1196..2452)
                     /gene="xapB"
                     /locus_tag="ROI21_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HDX5112811.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDSEKITHRSVTH"
     gene            complement(2512..3345)
                     /gene="xapA"
                     /locus_tag="ROI21_003945"
     CDS             complement(2512..3345)
                     /gene="xapA"
                     /locus_tag="ROI21_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HDX5112812.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFSTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            3594..4358
                     /locus_tag="ROI21_003946"
     CDS             3594..4358
                     /locus_tag="ROI21_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112813.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNFYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(4397..5323)
                     /locus_tag="ROI21_003947"
     CDS             complement(4397..5323)
                     /locus_tag="ROI21_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5112814.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWDVVRGQGRALMAGREGDPLYQI"
     gene            5413..6411
                     /locus_tag="ROI21_003948"
     CDS             5413..6411
                     /locus_tag="ROI21_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDX5112815.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKHQTEQLQAQQESRANKA"
     gene            complement(6408..6626)
                     /locus_tag="ROI21_003949"
     CDS             complement(6408..6626)
                     /locus_tag="ROI21_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDX5112816.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(6628..8643)
                     /gene="ligA"
                     /locus_tag="ROI21_003950"
     CDS             complement(6628..8643)
                     /gene="ligA"
                     /locus_tag="ROI21_003950"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDX5112817.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLTQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIIINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(8714..9712)
                     /gene="zipA"
                     /locus_tag="ROI21_003951"
     CDS             complement(8714..9712)
                     /gene="zipA"
                     /locus_tag="ROI21_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDX5112818.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQSAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            9942..10703
                     /gene="cysZ"
                     /locus_tag="ROI21_003952"
     CDS             9942..10703
                     /gene="cysZ"
                     /locus_tag="ROI21_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDX5112819.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            10888..11859
                     /gene="cysK"
                     /locus_tag="ROI21_003953"
     CDS             10888..11859
                     /gene="cysK"
                     /locus_tag="ROI21_003953"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDX5112820.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            12243..12500
                     /gene="ptsH"
                     /locus_tag="ROI21_003954"
     CDS             12243..12500
                     /gene="ptsH"
                     /locus_tag="ROI21_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDX5112821.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            12545..14272
                     /gene="ptsI"
                     /locus_tag="ROI21_003955"
     CDS             12545..14272
                     /gene="ptsI"
                     /locus_tag="ROI21_003955"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDX5112822.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            14313..14822
                     /gene="crr"
                     /locus_tag="ROI21_003956"
     CDS             14313..14822
                     /gene="crr"
                     /locus_tag="ROI21_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDX5112823.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         3762 a         3841 c         3552 g         3672 t
ORIGIN      
        1 ttgcctgatg cgcttcgctt gtcagaccaa ctatgtccgg cagttcccgt gcctctcatc
       61 tactgcgcca acgccgccag cagtttaacc agcgcgggcg tcacctgctg cgtttcataa
      121 acaatatata agtctgcagg gatgcgttct ttgagcggac ggaatatgac accgggccag
      181 ttcatctgcg cgtagctgtc cgctatcaat gtgataccaa tacccatact gaccatggcg
      241 agtaccgttt gcggttcatt aacttcgcga ataacaaccg gtgaaaatcc cacctgctgg
      301 caaactcgct gcaaaaaatc ccagtcagtg tacacgggcg gcattgtaac aaaatactcg
      361 tcacgtagcg cttccagcgg aacggtggaa aatgatgaga gatgatgctc ttcaggcatc
      421 gccaccagaa acgccgattc atgcaaccgt aagctggtaa aacctgccgg tggttctgtc
      481 gccattctcc agattccggc atcgagttca cgtcgttcca gcagggccat ttgcatcgcg
      541 ggcatctttt cgcgaaaaag aacgtcaacg ttaggatttt ccctgaggaa tcgccgcata
      601 accggacgca tccgtcccca cattgccgtt cccacaaccc cgagttcaat ccgccctgct
      661 tctccccgac ctatttgttc aacccgagcc aatacattat ttgcattcac cagcaatcga
      721 cgcgattctt ccatcaagat tttgcccgcg tgtgtcagta cgacgctgcg cgaatggcga
      781 ataaaaagct gcgtgccgag ttgattttcc agctctttaa tatgaatact gagcggaggc
      841 tgagacatat ttaaacgcgc tgctgcgcga ccaaaatgca actcttccgc tacggcaaga
      901 aaataacgga gcaacttaag atctgttcta tatacgcgtt ccataattaa gtgctccata
      961 taataaatac atcgtattaa attatcatgg cataaagtat ttcaccacca gatatccgac
     1021 atacataaac acgatggata atatacccgg caatacattt ggaacaggaa taggtaatcg
     1081 cacgattgta aacaaagcac caatgccaaa accaacgcca gaagaaaata aaatatcttt
     1141 cactaattaa cctttatcat aaaagcagcc ccaaggaaca gagctgcgaa tccttttaat
     1201 gtgtaactga acgatgcgtt atcttttcag agtcatggtt atatttaaac ccaaagaaaa
     1261 atatcactgc gaggaaaaga gcataccctg caaacatcag ccaaatggta tgccagtctt
     1321 ttacgccatc caccgagaag taatccactg ccataccgct cagaatcgag ccaacccatg
     1381 cgccgacgcc atttaccatc gtcataaaaa ggccctgcgc gctggcacga atgctggaat
     1441 caacttcctg ttcgacaaat accgaaccag aaatattgaa gaaatcgaat gcacagccat
     1501 aaacaatcat cgacagcagc agcaaaataa atccggttgt tgacggatcg ccataggcga
     1561 agaagccaaa gcgcagcgtc caggccacca tactcatcag catgacggtt ttaatgccaa
     1621 atcgctttaa aaagaatggg atagtcagta taaagcccac ttctgccatc tgtgaaactg
     1681 acagtaaaat ggagggatat ttcaccacaa aactgtcagc aaactcagga ttacgggcga
     1741 aatcatgcag gaacgggttg ccaaaaacgt tggtaatttg cagcaccgcg cccagcatca
     1801 tggcgaagag gaaaaagata gccatgcgtg gatttttaaa cagcacgaag gcatccagac
     1861 ccagcttgct ggcaagcgat gtagtcgctt ttttctccgc aaccggaatc ttcggcaaag
     1921 tcagcgcata agccgacagc agcaatgacg caccggacgc gatatacagc tgcagactac
     1981 tcaattccag atgcagcagg cttactgccc acatggcgac aatgaacccc accgtaccaa
     2041 aaacgcgaat gggcgggaaa gcggtcaccg ggtcaagccc tgcctgggca agacaggaat
     2101 aagagacgct gttcgataac gcaatagtcg gcataaacgc catcgcattg actaacatca
     2161 cccaaaacat catatccgga tcagttacgg atgccgcata aaaaagtacg cccgcacaca
     2221 ccaggtgaca cagcatgtat gcacgttctg cacgcagcca tttgtctgcg atgatcccca
     2281 ttataccagg cataataatc gcggcgatcc ctttggaact gtaaaccatg ccaacattag
     2341 cgccggtgaa atgaagggta ttaatcatgt aagaaccgag ggtaaccagc cagctccccc
     2401 agataaaata ttgcaaaaag gacattacct ttaagcgcaa agcgatgctc ataatcgttt
     2461 ccttgtcgca tcatttggcc tcacccgcga ggccgtttct tattgttttt ctcaggcaat
     2521 tttgcgcaga aagccacaaa taaggttgat gaagttctgc ttagagagct ccgcagccgc
     2581 cagcgtttgc gcatgtgaca atttcacatc gcttaaacct tccgccatgt tggtaatcgc
     2641 agagaccgca acgactttaa gttcgcaatg gcgagcagaa ataacctcag gcaccacaga
     2701 cataccaaca acatccccac caataatttg catcatgcga atttccgccg cagtctcgaa
     2761 attcggaccc ggatacgaga cgaacacgcc ctccgtcaga gggaacccct cttctttcgc
     2821 cactttttgt aacagtgcgc ggtattccgc atcgtaggca ttcgccagcg agaagaagcg
     2881 ctcaccaaaa cgttcatcat taagacccac catcggcgtt cccggcatgg tgttgatgtg
     2941 atctttcaat gcgaccagac tgccggcccc cacttcagga cgcagtgagc cagccgcatt
     3001 ggtgctgaac agcaactcgc agcccagcaa cttaaaggta cggattgcat ccgtcatgat
     3061 ggtcatccca cgaccttcgt agaaatgtcc gcgacctttc atgcacacca ctggcacccc
     3121 ctggagataa cccagcacca gctcacccgc atggccatgt acggtactta ccgggaaccc
     3181 aggcagcttt tcgtaggaaa ttgcgaccgc gttctcaatc tgatcggcca gcgcgcccag
     3241 cccggaacct aaaataaagg ccactcgtgg cgtgaaatca ggtttataag tcttgataat
     3301 atctacgcaa tacagtgggt tatgagaaaa ctgagcctga tacatatata tccttttttt
     3361 gtagggtgga atctaacgcc tttttataac gagattgccc taactcatac caatacactt
     3421 ttttcatgta tccgatagtt attcgatatc gatatattta tattttttca ctattaatag
     3481 gcggaaaaaa ctgtattggt actctccttt ccattgcatt aacaatatgc cgcttttaat
     3541 caaccttttt attaagcaat caactaataa cgatcggcat agggaattat gctatgaaaa
     3601 aacatctttt tactctgaca ctctcttctg tattggcgat accagcagta tctcatgcag
     3661 aatttaaagg cggttttgca gatatcggcg tgcattatct ggactggacc agccggacta
     3721 ccgaaaaatc gtcgaccaaa tcacacaaag atgattttgg ttatcttgaa tttgaaggtg
     3781 gcgctaactt tagttgggga gaaatgtacg ggttcttcga ctgggaaaac ttctataacg
     3841 gtcgccataa taaaccaggt agcgagcagc gttatacctt taaaaatacc aaccgtattt
     3901 acctgggtga caccggattt aatttctact tgcatgcgta tggcacctac ggttctgcga
     3961 atcgcgtgaa tttccacgac gatatgttcc tgtacggtat cggctacaat ttcaccggca
     4021 gcggttggtg gttcaaaccg ttctttgcaa aacgctatac agaccaaacc tattataccg
     4081 gcgataacgg ttatgtcgcc ggttgggttg caggttacag ctttatgctg ggcagtgaga
     4141 aattcaccct caccaactgg aacgagtacg agtttgatcg tgatgccacc tacgcggcag
     4201 gtaatggcgg caaagaaggt ctgaacggtg ccgtcgcact ttggtggaat gcaacttcac
     4261 acatcaccac agggattcag tatcgttatg cggatgacaa actgggcgaa gatttttacc
     4321 aggatgcgat aatctactcc atcaaattta acttctgatt gattactgcc ggggctcccg
     4381 gcagtatgaa ttcactctat atctgataca aaggatcccc ttcccgccca gccattagcg
     4441 cacggccctg gcctcgtacc acgtcccata acgcttgtgc ggcggtggaa agtgaacggt
     4501 tcttcctgcg caccagcatc aactggcgtt ccaccaccgg cgtaatgcgc tttaccacca
     4561 gcgggctacc ttcaggcaat ggtagcgcca gtgcgggaag aatactgatg ccgatgcctg
     4621 cggctaccat cgggaacagc gtcgccggat gaccaatctc ctgcacaata ttcgcctgaa
     4681 taccgttgcg cgccagagct gcgtcaatca gcggtcggct gcctgacgcg taatcctgca
     4741 acaccaattt cgctccctgt aatgcctgcc agggtacata atcttccgcg gccaaagcgc
     4801 tatcgcgatg gcaaagcaag aagaaaggct cggaaagaat cgcttcacat tgcagatcgc
     4861 tcactgggcc tggatcgatg acgatgccaa aatcgacatc tccctggcga atactttcca
     4921 tcacccactg ctgcggacga tcgtgcagga caaactgaat atctggatag cggcgatggc
     4981 tttcagcaat gcattgcgga ataagatgcg cggaaatggt ctggctggca gcgacccgaa
     5041 cttttccgct cagttgttgc cccatacgcc cggtatcgcg caacgtgctg ttaagttcat
     5101 ccagcagtcg ctcaagacgc aaagccaact gctgccctgc atctgtaagc accacttcac
     5161 gcgtggttct gtccagcagg cgaacaccag tatgattttc cagctccttc acactgtgac
     5221 ttactgccga ctgactcagg ccgatacgct ctcctgcacg actaaaactt ttctcctgcg
     5281 ctactgtgac gaaaaccttt aattgcttta aagaataatt catctgtttt cttcatgaat
     5341 ggatgcaata aatcaatttt atttctcaaa ctggaagaag cacaatagaa ccatcgatca
     5401 tctggagtct ttatgaaact ttttcgtatc ctcgatcctt tcaccttaac cctgatcacg
     5461 gtggtgttgc tggcctcttt ctttccggcc agaggcgatt tcgtcccctt ctttgaaaat
     5521 ctgaccaccg cagctattgc cctgctgttc tttatgcacg gcgcgaagtt gtcacgtgaa
     5581 gcgattattg ctggcggtgg tcactggcga ctgcatttgt gggtaatgtg cagcaccttt
     5641 gtactgtttc cgattctggg cgtgctgttt gcctggtgga aaccggtaaa tgtcgacccg
     5701 atgctctact ccggttttct ctacttgtgc attctccccg ctaccgtgca gtctgcaatc
     5761 gcctttactt caatggcggg cggtaacgtc gcggcagcgg tttgttctgc gtcagcatcc
     5821 agcctgttgg ggattttcct ttcaccattg ctggttggtc tggtgatgaa tgttcacggt
     5881 gcgggaggca gccttgagca ggtcggtaaa attatgctgc aattgctgct gccgtttgta
     5941 ttggggcatc tttcccggcc gtggattggt gactgggtgt cgcgcaataa aaaatggatt
     6001 gcgaaaactg accagacgtc cattctgttg gtggtttata cagcgttcag cgaagccgtc
     6061 gttaatggta tctggcataa agttggctgg ggatcattgc tgtttatcgt ggtggtcagc
     6121 tgcgttcttc tggctatcgt gattgtagtt aacgtcttta tggcacgccg actgggcttc
     6181 aataaggcag atgaaattac tatcgtcttt tgtggttcga aaaagagtct ggcaaatggc
     6241 atcccgatgg caaacattct gttccccaca tcggtgatcg gtatgatggt gctgcctctg
     6301 atgattttcc atcagatcca attgatggtc tgtgcggtgc tggcgcgtcg atacaaacac
     6361 cagaccgaac agttacaggc gcagcaggaa agccgcgcca ataaagctta aagaggacgc
     6421 ttcaggggct ggaccagttg cgtcagcccc tcagttttaa tcagcagcgt gatttgcatt
     6481 agctcaccga gcttccctgc cgggaactca tctttgcggg caaaccacag aagatactcc
     6541 tccggcaggt cgattaagcg acgccctttg tatttgccaa acggcattat cgtattggct
     6601 atttcaatca gctgctcttt ttccatctca gctacccagc aaacgcagca tttccgcttc
     6661 gtcgataact tctatgccca gttcctgcgc tttcgccagt ttagatcctg cagcttcacc
     6721 cgctatcacc agatcggttt tcttcgacac gctgcccgcg actttcgcgc ccagttcgac
     6781 cagtcgagct ttagcgtcat cacgcgacat ctggcttaag ctgcccgtaa gcaccacggt
     6841 tttaccagca aacgggctgt caatctcttc cgcgttgata atgatcggct caggccagtg
     6901 aacaccttcc gccaacagct cgctgatgac attgcggttg ctttcttcgg caaagaagtt
     6961 gtgaacgtgg gaggcaacga caattccaac atcaggcacc ttttgcagct cttcaatcga
     7021 agcggcttcc agcgcttcca gcgtgccgaa atatgccgcc agacctgctg cggtggcctc
     7081 gccgacttca cggatgccaa gtgcatagag gaagcgagca aaggtggttt ctttcgcttt
     7141 ttccagcgcg ttaaccacgt tttgtgccga ttttggcccc atacgctcca gtccggtaag
     7201 tttgcctgcg gtgagtttga acagatccgc cggagtgtgg acatattctt tttcaaccag
     7261 ctgatcgatg attttgtcgc ccataccgtc aacgtccatc gcacggcggg aaacaaagtg
     7321 tttcagcgac tctttacgct gcgcaccgca aatcaggcca ccggtacagc gggcaaccgc
     7381 ttcaccttcc acacgctcga cgtcagaacc acataccgga caatgcgtcg ggaatacaac
     7441 ctcacgggta tcttccgggc gttcagaaag cacgacgttc accacctgcg ggatcacgtc
     7501 gcctgcgcgg cgaatcacca ctttatcgcc aatgcgtaaa ccaagacgtt cgatttcatc
     7561 cgcattgtgt aaggttgcgt tactcaccag cacgccagca acatggacag gttccagacg
     7621 cgcaacaggc gtaatcgcgc cagtacgccc aacctgaaac tcgacgtcac gcacaaaggt
     7681 catctgttcc tgcgccggga atttaaacgc taccgcccaa cgcggggcac gcgcgacaaa
     7741 gccaagctgc tcctgctgtg tcagtgagtt gaccttaatc accacgccgt cgatatcaaa
     7801 gcccagcgtc gggcggtctt cttccacttt gtgatagaac gccagcactt cttccgccga
     7861 ttcacaaagc gttacccggt cgctgaccgg caaaccccac tttttaaatt gcagtaaacg
     7921 tccaagatga gtatccggca gctcaccacc ttccagaaca ccgacgccat agcagaaaaa
     7981 agtgagcggt cgcttcgctg taatacgcgg atcaagctga cgcagtgaac cagctgccgc
     8041 attacgtggg ttagcaaaca ctttcccgcc cgtacgtcgc gcatcttcgt taatcttttc
     8101 gaagcccgcc tgtggcagga acacttcacc acgtacttcc agacgcgccg ggatattctc
     8161 tccgtgcagc ttcagcggaa tggcgcgaat agtacgcaca ttagacgtga tatcttcccc
     8221 ggtggtgcca tcgccacgag tcgcggcact gactaaaacg ccattttcat acagaatact
     8281 gacagcaagg ccatccagct tcagctcaca gcaccaggtg actttctcgt tgcttttcag
     8341 acggtcctgc acacgtttgt tgaaagcaag aaagctttct tcatcaaaaa cgttatccag
     8401 cgacaacatt ggtacttcat ggcggatctg gctgaaagcc gccagcggcg cagcacctac
     8461 acgttgggta ggcgaatcag gcgtaatcag ttctggatgt ttggtttcca gctcgcgcag
     8521 ttcgcgcatc agcctgtcat attcagcgtc gggaatttcc ggcgcatcca tcacatgata
     8581 aaggtattca tgatggcgaa gcgtcgttcg cagttctgtc agttgttgtt cgattgattc
     8641 catatcgcac catcaatgct aaaaaccccc gacaagcggg ggttcgaaga ggagttaatt
     8701 tgccttaagt gtatcaggcg ttggcgtctt tgacttcgcg gatgatgtcc tggtactcgc
     8761 gcaatttctg cggagtcatc atacggcgct gatcgtcaag cactacaccg cccacttcat
     8821 cggcaatatg ctgcgcagat tgcagcatca gcttgaagtt ctgcagctcg tcaccgtaag
     8881 acggtacctg cataaagata gtgacacccg gagtagtgaa atccttcatt tcaggatcaa
     8941 aggttcccgg tttcaccata ttcgccaggc tgaataacgc cgggccgctg ccatccgggc
     9001 taagatgacg atggtaaata ttcatatcgc caaaaatgaa gcccgcttgt tgaatgctgt
     9061 taagaagcag ttcaccgttt agctcgctac cgtgatgcgc cgcgacgttc atgataatca
     9121 ccgcttcttt gcgcttcggt ttatccataa ctggagccgg ttccgctaca ggctcaggct
     9181 gtggtgccgc tacgggttct gcagactgga aagcctgttg tgccggttgc ggtgctgaat
     9241 gcactggctg cggcgctgac gcgacctgtg gcgaaactgg ctgctgcaac ggctgttcag
     9301 gctgcggctg ataggcaggc tgctgcacgg gttgctgcac cggctgcgct ggacgcggag
     9361 catgttgcgg cggtacctgc gcttcaggcg gctgctggac cggttgacgc ggctgcgcag
     9421 acgcataagg cggttggtac tggtgttgcg gcgacggacg agcagcctca tgctcctggg
     9481 cgttagccgg ggcatgattc acgcggtgaa cacgaacctc accaacgccc tcatcatctt
     9541 cgacatcctc gtcataagaa tcgtcgtcac gttttgactt cattcgtttt aatggccgat
     9601 cgcggaacat agaagatcgt tctttacggc tggtccagaa accatgtacc agtaaagcga
     9661 ttatggcgat cgcgccaaca atgattaata tcagacgcaa atcctgcatc attatattct
     9721 ctgttgttct aacaccttgc caccacggca aacatttact cactaagagt atttgccgat
     9781 tacctcaagt gcaagtgcac tattaacttt cacagcacaa agatagatga aatcgtgctt
     9841 tttgctgttt tttcgaacat atcctaactg tccattgcgc aattacccgg taaaatacgc
     9901 agaattttcc tgggattggt caaaaggagc tcatcctgat tatggtttca tcattcacat
     9961 ctgccccacg cagcggtttt tactattttg cgcaaggctg gaagctcgtc tcgcaacctg
    10021 ggattcggcg tttcgttatt ttaccgctgc tggtcaatat tttgttgatg gggggcgcat
    10081 tctggtggct atttacgcaa ctcgatgtct ggatcccgac tctcatgagt tatgttccgg
    10141 actggttaca atggctgagc tatctgttgt ggcctctggc agtcatctct gtgctgttag
    10201 tgtttggcta tttcttctca acgattgcta actggattgc cgctccgttc aatggtttat
    10261 tggctgaaca actggaagca cgattgactg gcgctacacc gccagatacc gggattttcg
    10321 gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg gcaaaaattt gcctggtatc
    10381 tgccgcgcgc aattgtatta ctaattcttt acttcatccc tggtattggg caaaccgtcg
    10441 cgccggtact gtggttcctg tttagcgcct ggatgttagc catccagtat tgcgattacc
    10501 ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa
    10561 tcaccaatat gcagtttggt gctttaacca gcctgtttac gatgatcccg ctgcttaatc
    10621 tgttcatcat gcccgttgcc gtttgtggtg cgacggcgat gtgggtcgat tgctatcgcg
    10681 ataaacacgc gatgtggcgg taacaatcta ccggttattt tgtaaaccgc ttgtgtgaaa
    10741 caggggtggc ttatgccgcc ccttattcca tcttgcatgt cattatttcc cttctgcata
    10801 tagatatgct aaatccttac ttccgcatat tctctgagcg ggtatgctac ctgttgtatc
    10861 ccaatttcat acagttaagg acaggccatg agtaagattt ttgaagataa ctcgctgact
    10921 atcggtcaca cgccgctggt tcgcctgaat cgcatcggta acggacgcat tctggcgaag
    10981 gtggaatctc gtaaccccag cttcagcgtt aagtgccgta tcggtgctaa catgatttgg
    11041 gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac tggttgaacc aaccagcggt
    11101 aataccggga ttgcactggc ctatgtcgct gccgctcgcg gttacaaact taccctgacc
    11161 atgccagaaa ccatgagtat tgaacgccgc aagctgctga aagcgttagg tgcaaacctg
    11221 gtgctgaccg aaggtgctaa aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc
    11281 gccagcaatc cagagaaata cctgctgctg caacaattca gcaatccggc aaaccctgaa
    11341 attcacgaaa aaaccaccgg tccggagatc tgggaagata ccgacggtca ggttgatgta
    11401 tttattgctg gcgttgggac tggcggtacg ctgactggcg tcagccgcta cattaaaggc
    11461 accaaaggca agaccgatct tatctctgtc gccgttgagc caaccgattc tccagttatc
    11521 gcccaggcac tggctggtga agagattaaa cctggcccgc ataaaattca gggtattggc
    11581 gcaggtttta tcccggctaa cctcgatctc aagctggtcg ataaagtcat tggcatcacc
    11641 aatgaagaag ccatttctac cgcccgtcgt ctgatggaag aagaaggtat tcttgcaggt
    11701 atctcttctg gagcagctgt tgccgcggcg ttgaaactac aagaagatga aagctttacc
    11761 aacaagaata ttgtggttat tctaccatca tcgggtgagc gttatttaag caccgcattg
    11821 tttgccgatc tcttcactga gaaagaattg caacagtaat gccagcttgt taaaaatgcg
    11881 taaaaaagca ccttttcagg tgcttttttg tggcctgctt caaactttcg cccctcctgg
    11941 cattgattca gcctgtcgga actggtattt aaccagacta attattttga tgcgcgaaat
    12001 taatcgttac aggaaaagcc aaagctgaat cgattttatg atttggttca attcttcctt
    12061 tagcggcata atgtttaatg acgtacgaaa cgtcagcggt caacacccgc cagcaatgga
    12121 ctgtattgcg ctcttcgtgc gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa
    12181 agtcgaaccg ccaggctaga ctttagttcc acaacactaa acctataagt tggggaaata
    12241 caatgttcca gcaagaagtt accattaccg ctccgaacgg tctgcacacc cgccctgctg
    12301 cccagtttgt aaaagaagct aagggcttca cttctgaaat tactgtgact tccaacggca
    12361 aaagcgccag cgcgaaaagc ctgtttaaac tgcagactct gggcctgact caaggtaccg
    12421 ttgtgactat ctccgcagaa ggcgaagacg agcagaaagc ggttgaacat ctggttaaac
    12481 tgatggcgga actcgagtaa tttcccgggt tcttttaaaa atcagtcaca agtaaggtag
    12541 ggttatgatt tcaggcattt tagcatcccc gggtatcgct ttcggtaaag ctctgcttct
    12601 gaaagaagac gaaattgtca ttgaccggaa aaaaatttct gccgaccagg ttgatcagga
    12661 agttgaacgt tttctgagcg gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac
    12721 gaaagctggt gaaacgttcg gtgaagaaaa agaagccatc tttgaagggc atattatgct
    12781 gctcgaagat gaggagctgg agcaggaaat catagccctg attaaagata agcacatgac
    12841 agctgacgca gctgctcatg aagttatcga aggtcaggct tctgccctgg aagagctgga
    12901 tgatgaatac ctgaaagaac gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg
    12961 caacatcctg ggcctgaaga ttatcgacct gagcgccatt caggatgaag tcattctggt
    13021 tgccgctgac ctgacgccgt ccgaaactgc acagctgaac ctgaagaagg tgctgggttt
    13081 catcaccgac gcgggtggcc gtacttccca cacctctatc atggcgcgtt ctctggaact
    13141 gcctgctatc gtgggtaccg gtagcgtcac ctctcaggtg aaaaatgacg actatctgat
    13201 tctggatgcc gtaaataatc aggtttacgt caatccaacc aacgaagtta ttgataaaat
    13261 gcgcgctgtt caggagcaag tggcttctga aaaagcagag cttgctaaac tgaaagatct
    13321 gccagctatt acgctggacg gtcaccaggt agaagtgtgc gctaacattg gtacggttcg
    13381 tgacgttgaa ggtgcagagc gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt
    13441 tctgttcatg gaccgcgacg cgctgcccac tgaagaagaa cagtttgctg cttacaaagc
    13501 agtggctgaa gcgtgtggct cgcaggcggt tatcgttcgt accatggaca tcggcggcga
    13561 caaagagctg ccatacatga acttcccgaa agaagagaac ccgttcctcg gctggcgcgc
    13621 tatccgtatc gcgatggatc gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg
    13681 tgcctcggct ttcggtaaat tgcgcattat gttcccgatg atcatctctg ttgaagaagt
    13741 gcgtgcactg cgcaaagaga tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc
    13801 gtttgacgag tcaattgaaa tcggcgtaat ggtggaaaca ccggctgccg caacaattgc
    13861 acgtcattta gccaaagaag ttgatttctt tagtatcggc accaatgatt taacgcagta
    13921 cactctggca gttgaccgtg gtaatgatat gatttcacac ctttaccagc caatgtcacc
    13981 gtccgtgctg aacttgatca agcaagttat tgatgcttct catgctgaag gcaaatggac
    14041 tggcatgtgt ggtgagcttg caggcgacga acgtgctaca cttctgttgc tggggatggg
    14101 tctggacgaa ttctctatga gcgccatttc tatcccgcgc attaagaaga ttatccgtaa
    14161 cacgaacttc gaagatgcga aggtgttagc agagcaggct cttgctcaac cgacaacgga
    14221 cgagttaatg acgctggtta acaagttcat tgaagaaaaa acaatctgct aatccacgag
    14281 atgcggccca atttactgct taggagaaga tcatgggttt gttcgataaa ctgaaatctc
    14341 tggtttccga cgacaagaag gataccggaa ctattgagat cattgctccg ctctctggcg
    14401 agatcgtcaa tatcgaagac gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg
    14461 gtattgctat caaaccaact ggaaacaaaa tggtcgcgcc tgtagacggc accattggta
    14521 aaatctttga aaccaaccac gcgttctcta tcgaatctga tagcggcgtt gaactgttcg
    14581 tccacttcgg tatcgacacc gttgaactga aaggcgaagg cttcaagcgt attgctgaag
    14641 aaggtcagcg cgtgaaagtt ggcgatactg tcattgaatt tgatctgccg ctgctggaag
    14701 agaaagccaa gtctaccctg actccggttg ttatctccaa catggacgaa atcaaagaac
    14761 tgatcaaact gtccggtagc gtgactgtgg gtgaaacccc ggttatccgc atcaagaagt
    14821 aattctt
//
LOCUS       DAPOZO010000113        14547 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPOZO010000113 DAPOZO010000000
VERSION     DAPOZO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.113"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            144..1484
                     /gene="wcaJ"
                     /locus_tag="ROI21_003957"
     CDS             144..1484
                     /gene="wcaJ"
                     /locus_tag="ROI21_003957"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDX5112824.1"
                     /translation="MSHLFPVIYLKSNQLWLVCEVSGLSFLYMHLLVALITLVVFQML
                     GGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTS
                     IGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLMESFRNQPWLGFEVVGVYH
                     DPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGARVKKLVHQLADTTCSVLL
                     IPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLIKRAEDIVLATLILLLISPVLC
                     CIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTKV
                     GNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKVKPGITG
                     WAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY"
     gene            1486..2964
                     /gene="wzxC"
                     /locus_tag="ROI21_003958"
     CDS             1486..2964
                     /gene="wzxC"
                     /locus_tag="ROI21_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDX5112825.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGASVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            3240..4520
                     /gene="wcaK"
                     /locus_tag="ROI21_003959"
     CDS             3240..4520
                     /gene="wcaK"
                     /locus_tag="ROI21_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDX5112826.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCNALILRESVSLELMKRSNITTAKVEDGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEAK"
     gene            4517..5737
                     /gene="wcaL"
                     /locus_tag="ROI21_003960"
     CDS             4517..5737
                     /gene="wcaL"
                     /locus_tag="ROI21_003960"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDX5112827.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDKLAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            5748..7142
                     /gene="wcaM"
                     /locus_tag="ROI21_003961"
     CDS             5748..7142
                     /gene="wcaM"
                     /locus_tag="ROI21_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDX5112828.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEKGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHIIERINCT
                     NGKINWGIGIGLAGSTYGNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVVDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAASFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGAALRMNFDMRKDVRGVFMAKRETLLSLANIHAVNETGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            7300..8295
                     /locus_tag="ROI21_003962"
     CDS             7300..8295
                     /locus_tag="ROI21_003962"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HDX5112829.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAIYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            8527..9420
                     /gene="galF"
                     /locus_tag="ROI21_003963"
     CDS             8527..9420
                     /gene="galF"
                     /locus_tag="ROI21_003963"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDX5112830.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDCEGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     VDIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            9792..10877
                     /gene="rfbB"
                     /locus_tag="ROI21_003964"
     CDS             9792..10877
                     /gene="rfbB"
                     /locus_tag="ROI21_003964"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDX5112831.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            10877..11776
                     /gene="rfbD"
                     /locus_tag="ROI21_003965"
     CDS             10877..11776
                     /gene="rfbD"
                     /locus_tag="ROI21_003965"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDX5112832.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIALALNKLNAVPTTAYPTPARRPHNSRLDTEKFQQNFA
                     LVLPDWQIGVKRMLNELFTTTAI"
     gene            11834..12712
                     /gene="rfbA"
                     /locus_tag="ROI21_003966"
     CDS             11834..12712
                     /gene="rfbA"
                     /locus_tag="ROI21_003966"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDX5112833.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQLSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLQPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            12717..13265
                     /gene="rfbC"
                     /locus_tag="ROI21_003967"
     CDS             12717..13265
                     /gene="rfbC"
                     /locus_tag="ROI21_003967"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDX5112834.1"
                     /translation="MNVIKTEIPDVLIFEPKVFSDERGFFFESFNQKVFEEAVGRKVK
                     FVQDNHSKSSKGVLRGLHYQLEPYAQAKLVRCMVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKHQLWIPEGFAHGFLVLSNTAEVLYKTNNYYNKENERNIIWNDNTIDIDWPEL
                     NINVILSTKDKCASHINKIDYF"
     gene            13272..14522
                     /gene="wzx"
                     /locus_tag="ROI21_003968"
     CDS             13272..14522
                     /gene="wzx"
                     /locus_tag="ROI21_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021523154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O102 family O-antigen flippase"
                     /protein_id="HDX5112835.1"
                     /translation="MSLLKNSIWNFGGYIIPTIVALPALGYIARELGAERFGLFALCT
                     AIVGYASIFDAGLTRAVIREIAFNRTNKSELNKIIATSNFAIIILGLVGAALLFMNID
                     NLVSLLKVSPRYKDDVVSSFKILTITIPLFLLNQIFVSYLEGMEDFFNINIQRCISSS
                     LVAGLPAFFIILHPTLTCAMIGLVCGRMVSLIISYYCAKEIVLTAGIRFHKQVLFRLL
                     KFGGWITVSNIISPVMSYFDRFILSNLLGAKSVGFYIAPSEFINRLINIPWALARALF
                     PKLSAAKTLSERKKNETIAYIMLSMVCIPAVIIGVGLSYTILNIWLGKEYAENSTYIL
                     QVLLIGFLFNSYAQIPFATIQAAGKAKITALIHCIEIIPYLVGLYFFIIHFGVIGAAY
                     AWTIRVSLDFVILLVASRGIYKSW"
BASE COUNT         3810 a         3212 c         3681 g         3844 t
ORIGIN      
        1 agatacggag aaaaaagagt aatatctgca atcaggctgg cgcgagttct gtttttgttc
       61 gggccggtct gattttcaca tactgtaagg ttattgcata ttcctcctgg tttcttccca
      121 ttttgaccca gttcttccca taaatatctc acttattccc tgtcatttat ctcaagtcta
      181 atcagctatg gctggtttgc gaagtcagcg gactgtcatt cctctacatg cacctgttgg
      241 tggcgctgat tacgctggtg gtgttccaga tgctgggcgg catcaccgat ttttatcgct
      301 catggcgcgg tgttcgggcc gcgacagaat ttgccctgct gctacaaaac tggaccttaa
      361 gcgtgatttt cagcgccgga ctggtggcgt tcaacaatga tttcgacacg caactgaaaa
      421 tctggctggc gtggtatggg ctgaccagca tcggactggt ggtttgccgt tcatgtattc
      481 gcattggggc gggctggctg cgtaatcatg gctataacaa gcggatggtc gcggtggcgg
      541 gggatttagc cgccggacaa atgctgatgg agagcttccg taatcagccg tggttagggt
      601 ttgaagtggt gggcgtatac cacgacccaa aaccgggcgg cgtttctaac gactgggcgg
      661 gcaatctgca acaactggtc gaggatgcga aagcgggcaa gattcataac gtctatatcg
      721 cgatgcagat gtgcgacggc gcgcgagtga aaaaactggt ccatcaactg gcggacacca
      781 cctgttcggt gctgctgatc cccgatgtct ttaccttcaa cattctccat tcccgtatcg
      841 aagagatgaa cggcgtaccg gtggtgccgc tgtatgacac gccgctttcc ggggttaacc
      901 gtctgataaa acgtgcagaa gacattgtgc tggcgacgct tatcctgctg ctgatctccc
      961 cggtgctgtg ctgcattgcg ctggcggtga aactgagctc accggggccg gttattttcc
     1021 gccagactcg ctacggcatg gatggcaagc cgatcaaagt gtggaagttc cgttctatga
     1081 aagtgatgga gaacgacaaa gtggtgactc aggcgacgca gaacgatccg cgcgtcacca
     1141 aagtggggaa ctttctgcgc cgcacctcgc tggatgaact gccgcagttt atcaatgtgc
     1201 tgaccggggg gatgtcgatt gtgggtccac gtccgcacgc ggtggcgcat aacgaacagt
     1261 atcgccagct cattgaaggc tacatgctgc gccataaggt gaaaccgggc attaccggct
     1321 gggcgcagat taatggctgg cgcggcgaaa ccgacacgct ggagaaaatg gaaaaacgcg
     1381 tcgagttcga ccttgagtac atccgcgaat ggagcgtctg gttcgatatc aaaatcgttt
     1441 tcctgacggt attcaaaggc ttcgttaaca aagcggcata ttgatatgag cttacgtgaa
     1501 aaaaccatca gcggcgcgaa gtggtcggcg attgccacgg tgatcatcat cggcctcggg
     1561 ctggtgcaga tgaccgtgct ggcgcggatt atcgacaacc accagttcgg tttgcttacc
     1621 gtgtcgctgg tgatcatcgc gctggcagat acgctttctg actttggtat cgctaactcg
     1681 attattcagc gaaaagaaat cagccacctt gaactcacca cgttgtactg gctgaacgtc
     1741 gggctggggc ttgtggtgtg cgtggcggtg tttttgttga gtgatctcat cggcgacgtg
     1801 ctgaacaacc cggacctggc accgttgatt aaaacattgt cgctggcgtt tgtggtgatc
     1861 ccccacgggc agcagttccg cgcgttgatg cagaaggaac tggagttcaa caaaatcggc
     1921 atgatcgaaa ccagcgcggt gctggcgggc ttcactttta cggtggttag cgcccatttc
     1981 tggccgctgg cgatgaccgc gatcctcggt tatctggtca atagtgcggt aagaacgctg
     2041 ctgtttggct actttggtcg caaaatttat cgccccggtc tgcatttctc gctggcgtcg
     2101 gtggcaccga acttacgctt tggtgcctgg ctgacggcgg acagcatcat caactatctc
     2161 aataccaacc tttcaacgct ggtgctggcg cgtattctcg gcgcgagcgt ggcagggggg
     2221 tacaacctgg cgtacaacgt ggccgttgtg ccaccgatga agctgaaccc catcatcacc
     2281 cgcgtgttgt ttccggcatt cgccaaaatt caggacgata ccgaaaagct gcgtgttaac
     2341 ttctacaagc tgctgtcggt agtggggatt atcaactttc cggcgctgct ggggctgatg
     2401 gtggtgtcga ataacttcgt accgctggta ttcggcgaaa agtggaacag cattattccg
     2461 gtgctgcaat tgctgtgtat tgtgggtctg ctgcgctccg tgggtaaccc gattggttcg
     2521 ctgctgatgg cgaaagcgcg ggtcgacatc agctttaaat tcaacgtatt caaaaccttt
     2581 ctgtttattc cggcgattgt tattggtggg cagatggcgg gcgcaatcgg cgtcacgctc
     2641 ggtttcctgc tggtgcagat tatcaacacc attctgagtt acttcgtgat gattaaaccg
     2701 gttcttggtt ccagttatcg ccagtacatc ctgagtttgt ggctgccgtt ttacctctcg
     2761 ctgccgacgc tggtggttag ttatgcgctg ggcattgtgc tgaaagggca actggcgctg
     2821 gggatgctgc tggcggtgca aatagccgcg ggcgtgctgg cgtttgtggt gatgattgtg
     2881 ctgtcgcgcc atccgctggt ggtggaagtg aagcgtcagt tttgtcgcag cgaaaaaatg
     2941 aaaatgcttt tacgggcggg gtgagttgct gttttcggtt ccgacaacgg ttgccggatg
     3001 cggcgtaaaa cgccttatcc atcctacggt tctgtgctcg ggacctgtag gcctgataag
     3061 acgcgtcagc gtcgcatcag gcactgaatg ccggatgcgg cgtgaacgcc ttatccggcc
     3121 tacggttctg tgctcgggtc ttgtaggcct gataagacgc gtcagcgtcg catcaggcaa
     3181 aaagcggcaa cgaacaaaac aaacgcagcc tcacctggca tcaaatttat gaggaaaaaa
     3241 tgaaattact tattctgggc aaccacactt gcggcaatcg tggcgacagc gccatcttgc
     3301 gcggcttact tgatgccatc aacatcctca atccacaagc cgaagtggac gtgatgagcc
     3361 gctatccggt cagttcttcc tggctgctca accgcccggt aatgggcgat ccgctgttcc
     3421 tgcaaatgaa acaacacaac agcgcggcgg gcgttgtcgg gcgcgttaaa aaagtcctcc
     3481 gtcgccgcta tcaacaccag gtattgctct cacgcgtcac tgacactggc aagctgcgta
     3541 atatcgccat cgcccaggga ttcaccgact tcgtgcgcct gctgtcaggt tacgacgcca
     3601 ttattcaggt cggcggatcg ttttttgtcg atctctacgg cgtgccgcag tttgaacatg
     3661 cgctttgcac atttatggcg aaaaagccgc tgtttatgat tggtcacagc gtcggtccct
     3721 tccaggatga gcaatttaac caactggcga actacgtttt tggtcactgt aacgcgctga
     3781 tcctgcgcga atcggtcagt ctcgaactga tgaaacgcag caatatcacc actgcaaaag
     3841 tggaagatgg cgtcgatacc gcgtggctgg tcgatcacca cacagaagat ttcaccgcca
     3901 gctatgccgt ccaacactgg ctggacgttg ccgcacaaca gaaaacggtg gcaattaccc
     3961 tgcgcgaact ggcaccgttc gacaaacgtc tcggcaccac tcaacaagcg tatgaaaaag
     4021 cctttgccgg ggtggtcaat cgcattctcg acgaagggta tcaggtgatt gcgctatcca
     4081 cctgtacggg catcgacagc tataacaaag acgaccgcat ggtggcgctc aacctacgcc
     4141 agcacatcag cgatcctgcc cgttatcacg tagtgatgga tgaactcaac gatctggaaa
     4201 tgggcaaaat tctgggggcc tgtgaactca ccgtcggtac gcgcctgcac tccgccatta
     4261 tctcaatgaa ctttgctact ccggcgattg ccatcaacta tgaacataaa tccgccggga
     4321 ttatgcagca gttgggacta ccggagatgg cgattgatat ccgtcattta ctggacggca
     4381 gcctgcaagc gatggttgcg gataccttag gccagcttcc ggcgctgaac gcacgactta
     4441 gcgaagccgt cagtcgtgag cgtcagacag gaatgcagat ggtgcagtcc gtactggaac
     4501 gcattgggga ggcgaaatga aggttggctt ctttttactg aaatttccgc tgtcgtcgga
     4561 aaccttcgtc ctcaaccaaa ttaccgcgtt tattgatatg ggctttgagg tggagattgt
     4621 cgcgctgcaa aaaggcgaca cacaaaacac ccacgcggca tggacgaaat acaaccttgc
     4681 cgccagaacc cgatggttac aggacgaacc tacgggcaaa gtggcgaagc tgcgccaccg
     4741 cgccagtcag accttgcgcg gcattcatcg taaaaatacc tggcaggcgc tcaacctcaa
     4801 acgctatggt gccgagtcgc ggaacctgat tttgtctgcc atttgcggcc aggtcgcaac
     4861 accgtttcgc gccgatgtgt tcatcgctca ttttggcccg gcgggggtaa ccgcagcaaa
     4921 actacgcgaa ctgggtgtca ttcgcggcaa aattgccact attttccacg gcattgatat
     4981 ttccagtcgg gaagtgctca accattacac tcccgaatat cagcaactgt ttcgccgtgg
     5041 cgacctgatg ttaccgataa gcgatttgtg ggccggaagg ctgcaaaaaa tgggctgccc
     5101 cagagaaaaa atcgccgtat cgcgtatggg cgtggacatg acgcgcttta gcccgcgtcc
     5161 ggtgaaagcg cccgcaacgc cgctggaaat catctccgtc gcacgcttaa ctgaaaaaaa
     5221 aggcctgcat gtggcgatcg aagcctgccg tcagttgaaa gagcagggca tggcatttcg
     5281 ctatcgcatc ctcggcattg gcccgtggga acgacgcctg cgtaccctca tcgaacaata
     5341 tcaactggaa gatgtggtag agatgccggg ctttaaaccg agccacgaag tgaaagcgat
     5401 gctcgacgac gcggatgtct tcctgttgcc atcggtaacg ggcgcggatg gcgatatgga
     5461 aggcattccg gtagcgctga tggaagcgat ggcggtcggc attccggtgg tttctactct
     5521 gcatagcgga ataccggaat tggtggaggc cgataaatcc ggctggctgg tgcctgagaa
     5581 cgatgctcgc gcgctggcgc aacgcctggc ggcatttagc caactggaca ccgacaaatt
     5641 ggctccggtc gtcaaacgcg cgcgcgaaaa agttgaacac gattttaacc agcaggtgat
     5701 caatcgagaa ctcgccagct tgctgcaagc tttatagagg agaacgtatg ccatttaaaa
     5761 aactctcccg acgcaccttc ctgacggcaa gctcggcgct tgccttcctc catacccctt
     5821 tcgcccgtgc acttcccgcc cgacaaagcg ttaacattaa cgactacaac ccacacgact
     5881 ggatcgcctc atttaaacaa gccttcagcg aagggcaaac agtcgtcgtg cctgccggat
     5941 tggtttgtga caatatcaac accggcatct tcattcctgc aggtaaaacg ttacacatcc
     6001 ttggaagcct gcgcggcaat ggcagagggc gatttgtctt acaggacggc agccaggtga
     6061 caggggagaa gggcggcagt atgcataaca tcaccctgga tgtgcgtggt tctgactgca
     6121 ccatcaaagg gctgacgatg agcggctttg gcccggtgac gcagatctat atcggcggca
     6181 aaaacaaacg ggtcatgcgt aacctgacca tcgataacct cactgtcagc cacgctaatt
     6241 acgccatctt acgccaagga tttcataacc agataatcgg tgccaacatc accaattgta
     6301 agttcagcga cttacagggc gatgccattg aatggaacgt ggcgattaac gaccgcgata
     6361 ttttgatatc cgatcacatc atcgagcgca tcaactgtac caacggcaaa atcaactggg
     6421 gcatcggcat aggccttgcg ggaagcactt acggtaataa ttacccagaa gatcaggcag
     6481 tgaaaaactt tgtcgtggcg aatatcacgg gatcggattg tcggcagttg atccatgttg
     6541 aaaatggtaa acattttgtt attcgtaata tcaaagcccg caatatcacg ccggatttca
     6601 gtaagaaagc aggcattgat aacgcgacag ttgctattta cggttgtgac aatttcgtgg
     6661 ttgataatat tgaaatgatt aatagcgccg ggatgttaat cggctatggg gtaattaaag
     6721 gcaaatatct ctcgataccg caaaatttcc gagtgaataa tattcaactg gataacaccc
     6781 atcttgctta taaattgcgc ggcatccaaa tctccgccgg gaatgctgcc tcctttgtgg
     6841 cactgactaa cattgagatg aagcgtgcct cgctggagct tcacaataaa ccgcagcatc
     6901 tttttatgcg taatatcaaa gtgatgcagg aatcctcagt tggagccgca ttgcgcatga
     6961 acttcgacat gcgcaaagac gttcgcggcg tctttatggc aaagcgtgag acgctgcttt
     7021 cacttgcgaa tattcatgca gtgaatgaaa cggggcaaag ctccgtcgat atcgacagga
     7081 ttaatcacca tattgttaat gtggaaaaga ttaactttag attgccggaa cggagagagt
     7141 agatttgcga ccattcataa acttactgga tataatgttc taaaagatta taatcctcac
     7201 actaactgtt aaagctgggg ctgggcagca ttgactactc agggaaggta ctcatcttta
     7261 tcttgtgttt cttacgtaac aaccagacta aagtgattta tgaacgataa cgttttgctc
     7321 ataggagctt ccggattcgt aggaacccga ctacttgaaa cggcaattgc tgactttaat
     7381 atcaagaacc tggacaaaca gcagagccac ttttatccag aaatcacaca gattggcgat
     7441 gttcgtgatc aacaggcact cgaccaggcg ttagccggtt ttgacactgt tgtactactg
     7501 gcagcggaac accgcgatga cgtcagccct acttctctct attatgatgt caacgttcag
     7561 ggtacccgca atgtgctggc ggccatggaa aaaaatggcg ttaaaaatat catctttacc
     7621 agttccgttg ctatttatgg tttgaacaaa cacaaccctg acgaaaacca tccacacgat
     7681 cctttcaacc actacggcaa aagtaagtgg caggcagagg aagtgctacg tgaatggtat
     7741 aacaaagcac caacagaacg ttcattaact atcatccgtc ctaccgttat cttcggtgaa
     7801 cgcaaccgcg gtaacgtcta taacttgctg aaacagatcg ctggcggcaa gtttatgatg
     7861 gtgggcgcag ggactaacta taagtccatg gcttatgttg gaaacattgt tgagtttatc
     7921 aagtacaaac tgaagaatgt tgccgcaggt tatgaggttt ataactatgt tgataagcca
     7981 gacctgaaca tgaaccagtt ggttgctgaa gttgaacaaa gcctgaacaa aaagatccct
     8041 tctatgcact tgccttaccc actaggaatg ctgggtggat attgctttga tatcctgagc
     8101 aaaattacgg gcaaaaaata cgctgtcagc tctgtgcgcg tgaaaaaatt ctgcgcaaca
     8161 acacagtttg acgcaacgaa agtgcattct tcaggttttg tggcaccgta tacgctgtcg
     8221 caaggtctgg atcgaactct gcagtatgaa ttcgtccatg ccaaaaaaga cgacataacg
     8281 tttgtttctg agtaatgcag acgagtcaga gaaaaaagga ccaggttgga ctacgattat
     8341 tcggaatgag gtactgatta aattatcagt atgtggattt ccgagtaatc cgagtaaccc
     8401 agatgctgat ggtgtgtagt gttatcgtaa ggtttaatca aagttatgct tggtatatag
     8461 ccataccgtt tggctaacct atgacaataa tttacgcact atcagccact tagaggtagg
     8521 aaaataatga cgaatttaaa agcagttata ccggtagcgg gtcttgggat gcatatgttg
     8581 cctgccacta aggcgatacc caaagagatg ctaccgatcg tcgacaagcc aatgattcag
     8641 tacattgttg acgagattgt ggctgcaggg atcaaagaaa tcctcctggt gactcacgcg
     8701 tccaagaacg cggtcgaaaa ccacttcgac acctcttatg aattagaatc tctccttgag
     8761 cagcgcgtga agcgccaact gctggcggaa gtacagtcca tttgcccgcc gggcgtgaca
     8821 attatgaacg tgcgtcaggg cgaaccttta ggtttaggtc actccatttt gtgtgcacga
     8881 cccgccattg gtgacaatcc atttgtcgtg gtgctgccag acgttgtgat cgacgacgcc
     8941 agcgccgacc cgctgcgcta caaccttgct gccatgattg cacgtttcaa cgaaacgggt
     9001 cgtagccagg tgctggcaaa acgtatgccg ggtgacctct ctgaatactc tgttattcag
     9061 accaaagaac cgctggattg cgaaggtaaa gtcagccgca ttgttgaatt catcgaaaaa
     9121 ccggatcagc cgcaaacact ggactcagac atcatggccg ttggtcgcta tgtgctttct
     9181 gtcgatattt ggccggaact tgaacgcact cagcctggtg catggggacg tattcaactg
     9241 actgatgcca ttgctgaatt ggcgaaaaaa cagtccgttg atgccatgct gatgacaggt
     9301 gacagctacg actgcggtaa aaaaatgggt tatatgcagg cgtttgtgaa gtatggactg
     9361 cgcaacctga aagaaggggc gaagttccgt aaaggtattg agaagctgtt aagcgaataa
     9421 tgaaaatctg accgaatgta acggttgata agaaaattat aacggcagtg aagattcgtg
     9481 gcgaaagtaa tttgctgcga atattcctgc cgttgtttta tataaacaat cagaataaca
     9541 acgagttagc aataggattt tagtcaaagt tttccaggat tttccttgtt tccagagcgg
     9601 attggtaaga caattagcgt ttgaattttt cgagctaagc gcgagtgggt aacgctcgtc
     9661 acatcgtaga catgcatgca gtgcactggt agcagtaaag ccaggggcgg tagcgtgcat
     9721 taatacctct attaatcaaa ctaagagccg cttatttcac tgcatgctct gaagtaatat
     9781 ggaataaaag agtgaagata cttgttactg gtggcgcagg atttattggt tctgctgtag
     9841 ttcgtcacat tataaataat acgcaggata gtgttgttaa tgtcgataaa ttaacgtacg
     9901 ccggaaacct ggaatcactt gctgatgttt ctgattctga acgctatatt tttgaacatg
     9961 cggatatttg cgatgctgct gcaatggcac ggatttttgc tcagcatcag ccggatgcag
    10021 tgatgcacct ggctgctgaa agccatgttg accgttcaat tacaggtcct gcggcattta
    10081 ttgaaaccaa tattgttggt acttatgtcc ttttggaagc cgctcgcaat tactggtctg
    10141 ctcttgatag cgacaagaaa aatagcttcc gttttcatca tatttctact gacgaagtct
    10201 atggtgattt gcctcatcca gatgaagtaa ataataacga agaattaccc ttatttactg
    10261 agacgacagc ttacgcgcca agcagccctt attccgcatc aaaagcatcc agcgatcatt
    10321 tagtccgcgc gtggaaacgt acctatggtt taccgaccat tgtgactaac tgttcgaata
    10381 actacggtcc ttatcacttt ccggaaaaat tgattccact agtaattctt aatgctctgg
    10441 aaggtaaggc attacctatt tatggcaaag gggatcaaat tcgtgactgg ctgtatgttg
    10501 aagatcatgc gcgtgcgtta tataccgtcg taaccgaagg taaagcgggt gaaacttata
    10561 acattggtgg acacaacgaa aagaaaaaca tcgatgtagt gctcactatt tgtgatttgt
    10621 tggatgagat tgtaccgaaa gagaaatctt atcgtgagca aattacttat gttgccgacc
    10681 gcccgggaca tgatcgccgt tatgcgattg atgctgagaa gattagccgc gaattgggtt
    10741 ggaaaccaca ggaaacgttt gagagcggga ttcggaagac agtggaatgg tacctgtcca
    10801 atacaaaatg ggttgataat gtgaaaagtg gtgcctatca atcgtggatt gaacagaact
    10861 atgagggccg ccagtaatga atatcctcct tttcggcaaa acagggcagg taggttggga
    10921 actacagcgt gcgctggcac ctttgggtaa tttgattgct cttgatgttc actctactga
    10981 ttattgtggt gattttagta acccagaagg tgtggctgaa accgtcaaaa aaattcgtcc
    11041 tgacgttatt gttaatgctg ctgctcatac cgcggtagat aaggctgagt cagaacccga
    11101 atttgcacaa ttactcaatg cgacaagtgt tgaagcgatt gcaaaagcgg ctaatgaagt
    11161 tggggcttgg gtaattcatt actcaactga ctacgtattc cctggaaatg gcgacacgcc
    11221 atggctggag acggatgcaa ccgcaccgct aaatgtttac ggtgaaacca agttagccgg
    11281 agaaaaagcg ttacaggaac attgcgcgaa gcatcttatt ttccggacca gctgggtata
    11341 tgcaggtaaa ggaaacaatt ttgccaagac aatgttacgt ctggcaaaag agcgtgaaga
    11401 attagccgtt attaatgatc agtttggtgc gccaactggc gcagagttgc tggctgattg
    11461 tacggcacat gccattcgtg tagcactgaa taaaccggaa gtcgcaggct tgtaccattt
    11521 ggtagccagt ggtaccacaa cctggcacga ttatgctgcg ctcgtttttg aagaggcgcg
    11581 caaagcaggc attgcccttg ctctcaacaa gctcaacgca gtaccaacaa cagcctatcc
    11641 tacaccagct cgtcgtccgc ataactctcg ccttgataca gaaaaatttc agcagaactt
    11701 tgcgcttgtc ttgcctgact ggcagattgg cgtgaaacgc atgctcaacg aattatttac
    11761 gactacagct atttaatagt ttttgcatct tgttcgtgat ggtggagcaa gatgaattaa
    11821 aaggaatgat gaaatgaaaa cgcgtaaagg tattatttta gcgggcggtt ctggtactcg
    11881 tctttatcct gtgactatgg ctgtcagtaa acagctatta cctatttatg ataaaccgat
    11941 gatctattac ccgctctcta cactgatgtt ggcgggtatt cgcgatattt tgattatcag
    12001 tacgccacag gatactcctc gttttcaaca actgctgggt gacgggagcc agtggggcct
    12061 gaatcttcag tacaaagtgc aactgagtcc ggatggtctt gcgcaggcat ttatcatcgg
    12121 tgaagagttt atcggtggtg atgattgtgc tttggtactt ggtgataata tcttctacgg
    12181 tcacgacctg ccgaagttaa tggatgccgc tgttaacaaa gagagtggtg caacggtatt
    12241 tgcctatcac gttaatgatc ctgaacgcta tggtgtcgtt gagtttgata aaaacggtac
    12301 ggcaattagc cttgaagaaa aaccgctaca accaaaaagt aattatgcgg taaccgggct
    12361 ttatttctat gataacgacg ttgtggaaat ggcgaaaaac cttcagcctt ctgcccgtgg
    12421 tgaactggaa attaccgata ttaaccgtat ttatatggaa caggggcgtt tatccgttgc
    12481 catgatgggg cgtggttatg catggctgga cacggggaca catcaaagtc ttattgaagc
    12541 aagcaacttc attgctacaa ttgaagagcg tcaggggttg aaagtttcct gcccggaaga
    12601 aattgcttac cgtaaagggt ttattgatgc tgagcaggtg aaagtattag ctgaaccgct
    12661 gaaaaaaaat gcttatggtc agtatctgct caaaatgatt aaaggttatt aataaaatga
    12721 acgtaattaa aacagaaatt ccagatgtac tgatttttga accaaaagtt tttagcgacg
    12781 agcgcggttt cttttttgag agctttaacc agaaggtttt cgaagaagcg gtaggccgta
    12841 aagttaagtt tgttcaggat aaccattcga agtcaagtaa aggtgtttta cgtggtcttc
    12901 attaccagtt agaaccctat gctcaagcaa aactggtgcg ctgtatggtc ggtgaagttt
    12961 ttgatgttgc ggttgatatt cgtaaatctt cgcctacctt tggaaaatgg gttggggtga
    13021 acttatctgc tgagaataag catcaattat ggattccaga agggtttgct catggatttt
    13081 tagtattatc taacactgct gaggttttat ataagacgaa taattattac aataaagaaa
    13141 atgaaagaaa tattatttgg aatgataata caattgacat tgattggccc gaattgaata
    13201 taaatgttat cctttccact aaagataaat gtgcatctca cattaacaaa attgattatt
    13261 tttaggaaaa tatgagttta ttaaaaaata gtatatggaa ctttggtgga tacattattc
    13321 caaccattgt cgctttgcct gcattaggat atatagctcg tgaattggga gcagaaaggt
    13381 ttggcttgtt tgctctttgt acagctattg ttggttatgc cagtattttt gacgcaggat
    13441 taacaagagc tgttattaga gaaattgcgt ttaatagaac taataaatct gaattaaata
    13501 aaattattgc aacatctaat tttgctataa ttatactggg tttagttggt gcagctcttt
    13561 tgtttatgaa cattgataac ctagtgtctt tgttaaaggt tagccctaga tataaggatg
    13621 atgttgtatc ttcgtttaag atactgacta taactatacc tttatttttg ctaaatcaga
    13681 tatttgtatc atatttggag ggcatggaag atttttttaa tataaatatt caacgatgta
    13741 ttagtagttc attagtagcg gggcttccag cttttttcat cattctacat ccaaccctga
    13801 catgtgcaat gattggatta gtttgtggtc gaatggtttc gttaattatt tcatattatt
    13861 gtgcaaaaga aatagtatta acagcgggta ttagatttca taagcaagtt ttgtttcgac
    13921 tgttaaagtt tggtggctgg attactgtaa gtaatattat tagtccggtc atgagttatt
    13981 ttgatcggtt tatattatct aatcttctgg gggcaaaaag cgttgggttt tatatagcgc
    14041 cttcagaatt tattaataga ttgattaata ttccctgggc tttggcaaga gcattatttc
    14101 caaaattaag tgctgcaaag acactttcgg aaagaaaaaa aaatgaaaca atagcctata
    14161 ttatgctcag catggtttgc atacctgctg taattattgg agtgggatta tcgtatacaa
    14221 tcctaaatat atggttaggt aaagaatatg ctgaaaattc aacttatatt ttgcaagttc
    14281 ttctgatagg atttttattt aattcatatg ctcagattcc atttgctaca atccaggctg
    14341 caggtaaagc gaaaatcacg gcattaatac attgcattga aataattcct taccttgttg
    14401 gattgtattt ctttattata cattttggtg taataggggc tgcatatgcg tggacaatta
    14461 gagtcagttt agatttcgtt attttacttg tagcatctcg gggcatatat aaatcatggt
    14521 aaaaaaaata acacatttgg tcaaaaa
//
LOCUS       DAPOZO010000114        14141 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPOZO010000114 DAPOZO010000000
VERSION     DAPOZO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.114"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(5..1849)
                     /gene="selB"
                     /locus_tag="ROI21_003969"
     CDS             complement(5..1849)
                     /gene="selB"
                     /locus_tag="ROI21_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDX5112836.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQVIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(1846..3237)
                     /gene="selA"
                     /locus_tag="ROI21_003970"
     CDS             complement(1846..3237)
                     /gene="selA"
                     /locus_tag="ROI21_003970"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDX5112837.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDIPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3335..3943)
                     /locus_tag="ROI21_003971"
     CDS             complement(3335..3943)
                     /locus_tag="ROI21_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDX5112838.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(4014..5150)
                     /locus_tag="ROI21_003972"
     CDS             complement(4014..5150)
                     /locus_tag="ROI21_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDX5112839.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQVAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5153..5515)
                     /locus_tag="ROI21_003973"
     CDS             complement(5153..5515)
                     /locus_tag="ROI21_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDX5112840.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6052..7965
                     /gene="mtlA"
                     /locus_tag="ROI21_003974"
     CDS             6052..7965
                     /gene="mtlA"
                     /locus_tag="ROI21_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDX5112841.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVEPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8060..9208
                     /gene="mtlD"
                     /locus_tag="ROI21_003975"
     CDS             8060..9208
                     /gene="mtlD"
                     /locus_tag="ROI21_003975"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDX5112842.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMSALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIRK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPLAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9208..9795
                     /gene="mtlR"
                     /locus_tag="ROI21_003976"
     CDS             9208..9795
                     /gene="mtlR"
                     /locus_tag="ROI21_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDX5112843.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9807..10016)
                     /locus_tag="ROI21_003977"
     CDS             complement(9807..10016)
                     /locus_tag="ROI21_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112844.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10300..10662
                     /locus_tag="ROI21_003978"
     CDS             10300..10662
                     /locus_tag="ROI21_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDX5112845.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            11223..11906
                     /locus_tag="ROI21_003979"
     CDS             11223..11906
                     /locus_tag="ROI21_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDX5112846.1"
                     /translation="MNLKKTLLSALIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVQAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTMNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMDNLQFDTVQPA
                     ETRQRKNKK"
     gene            11950..>14141
                     /locus_tag="ROI21_003980"
     CDS             11950..>14141
                     /locus_tag="ROI21_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112847.1"
                     /translation="MNKIFKVIWNPATGSYTVASETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGVQAQAGRDNGQGVNYGQGTGTGTGWVAIGEDAKANSFTDTGGGSSTAVGYHA
                     TADGRWSTALGAKTHSLGEASVALGINTTSAGERSLAIGASATSTGGFSIALGRYANS
                     TGEFSIAQGDYAETGADDAIAFGRESKALGIMSIALGATANASKEYAMALGASSAASA
                     ANAIAVGRNSAAAGVDSLAFGRQSAANAANAIAMGAESKAAENATAVGTNAEANGLNS
                     IALGSGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIA
                     LGQGSYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAG
                     DISTTSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALT
                     GLDNNTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVAT
                     NTANITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTDATSKITNVKDGDLTAGS
                     TDAVNGSQLKTTNDAVAANTTNIATNTTNITNLTDAVDSLGDDSLLWNATAGAFSAAH
                     GTDATSKITNVKDGDLTAGSTDAVNGSQLKTTNDAVAANTTNIATNTTNITNLTDAVD
                     SLGDDSLLWNATAGAFSAAHGTDATSKITNVTAGDLTAGSTDAVNGSQL"
BASE COUNT         3582 a         3718 c         3571 g         3270 t
ORIGIN      
        1 ttccttattt ttccggaaat aataatgcgt cgcgcaataa atgatcattt ccccgacgac
       61 gcgtaaaacc aatgcggtca aaatattcca gaatttgaat tgccagtttt cgaccaacac
      121 ctaaacgatc gcggaaatcc gccgcgcagg ttgaaccaca ttcctgatcg agatcgcgga
      181 tcatattggc aaattcgaca atccgatcgt tacggtaata acgatcttta acgatcgccg
      241 taattatccc ctgctgggcc gcctggcgta gcgtcaggcg cattgcctgc tcgtcggttc
      301 ccgtctcttt tgccaggtca cgcacccacc acggttcgtc accaaacagc ggctctgctt
      361 tttgccagat gacctgctgc tcttcgctga agcccgcttt atgatccggc agatgcagcc
      421 aaccgtgatg actgaggatg tcgccgcttt cgcgcatctt ttcaatcagc aacagtacca
      481 gcgcttcatc ttccattggc aacgccatac gtcgcagacg ttcgcgccca ggaccaggtt
      541 catcgcgatg ttgctcatga taagtcgcta atgtgtcgag aattttccgc tgccagcggg
      601 cggcaaccgg cgcattcaac aagctataac cagcctgaat atagccaggc tgttgcagca
      661 gttcgcgcat cccttcgccg ttgagctgcc gtgcccaggc gaaatccgca aggttaaccg
      721 ctccgcgttc cagatgaaca gataacgcat cggcatcgct ctgcgcccgt gcaagagacg
      781 ccagccattg cagatattcc ggcttacgct taccgcgacg cggcgggtta agcatcacca
      841 cacgcgcccc cgccagcgtg ttgcgggccg agatatcgcg cagcaccaag cgatcgttat
      901 ccgccagcca tagcggcgtg tcgaacacca gctcggcaag gttatcttcc agcagcgaaa
      961 cgcgtcccgt gacgtggctg gcggcgtggt gaatatgcag cggctgccac tgggtcagcg
     1021 gtgtatgggt ttgaagctcg acaatcaccc gtgtgaaagg ctccggcggc gcatcggcaa
     1081 gcagccagtc gccacggtta atctgctctt tttccgcatc acccgcgatg ttaagcgcga
     1141 tacgctgccc ggcatgggca gtttctgttg gctgattttg cgcatgtagc gcacgcacgc
     1201 gcatcggttt atttacacca gtcagccaga gtgaatcgcc taccttcact tccccgctta
     1261 acgccgtacc ggtgacgacc agcccggcac cttttacggt aaatgcacgg tcgatcgcga
     1321 ggcggaaact atgttggctg gcgtgttcac gttctggtaa ctgaagcaaa tgctcgcgca
     1381 gggcatcaat tccccgacct tcggttgctg cggtgataaa cagttttgcc tcagcaaaac
     1441 cgtattcccg cagcacctcc tttacctggc gttcaacctc attaacacgc gcttcgtcca
     1501 cacgatcggc tttggtcagc gccactgtca gcatcgggtt accggtcagc tgcaaaatcg
     1561 ccagatgctc acgagtctgt gccatcacgc cgtcatcgca cgccaccacc aacagcgcgt
     1621 gatcaatacc accaacgccc gccagcatgt tggaaagaaa cttttcatga ccgggtacgt
     1681 cgataaaacc aggcacacgg ccatccggct gcggccagta ggcatagccg agatcgatgg
     1741 tcatgccgcg ctttttttct tccggcagac ggtcggcatt tacgcccgta atcgcctgca
     1801 ataaggttgt tttgccgtgg tcaacgtgtc cggcagtcgc aataatcatt tcaacaacat
     1861 ctccaaaaac cgttgctcat cttctaggca gcgtaaatcc agccacaatc gtccgtcata
     1921 aatacgacca atcaccggca ctggcaattc acgccagcgg gcggctaatg actcaaggtg
     1981 gctaccgcgt ccatcatggg gtgtaaacgt taatgccgcg ctcggcaggc gatcaaccgg
     2041 cagcgaacca ctgccaatct gcgaaagaca tggcataacc tgtaccgcaa actccgcgcc
     2101 gtaatgtgcg gcaaggggag cctgtaaacg ttgtgcctgg atttgaatga cctctgcgct
     2161 gcgggtaagc aggcgcaggg tcggtaattt ttcactcaga gcttcagggt gtaaataaag
     2221 acgcaacgtg gcttccagcg ccgcgagggt cattttatcc gcgcgtaatg cacgcttcag
     2281 cgggtggctt tgcaggcggg cgatcatctc ttttttacca acgataatcc ctgcctgcgg
     2341 cccgcctaac aacttgtcgc cggagaagct caccagactg acgcccgccg caatcaactc
     2401 ctgcggcatt ggctctttcg gcaaaccgta ctggctaaga tcaaccagcg agccactgcc
     2461 taaatcagtc accacgggaa tatccagttc tttgccaagc gccaccagtt cagcttcgtc
     2521 tatcgctttg gtgaacccct gaatgctgta gttactggta tgtactttca tcaacagtgc
     2581 ggtattttca ttcaccgcct gacgataatc attcgcgtgc gtgcggttgg tggtccctac
     2641 ttcgtgtagt gtgcagcctg cctgacgcat aacatcggga atacgaaacg cgccgccaat
     2701 ctccaccagt tcgccgcgag ataccaccac ctctttccca ctggcagtgg ccgccaacat
     2761 caacaatacc gccgccgcat tgttattgac gatacaggca tcttccgctc ccgtaatgcg
     2821 gcacagcagc tgcgccagcg cccgatcgcg atgtccgcgt ccggcgtcgt ccagatcgta
     2881 ctcgagggtc actggcgaac gcatagcctg cgcaacggct tccaccgcgg cttctgcctg
     2941 taaagctcgc ccaagattgg tatgcagcac ggttcccgtc aggttgatca ccggacgcag
     3001 cgcgctctgc gcctctttcg tcaaccgggc atcgacttct tgcgcccagt tttcacacca
     3061 tgcgggcagc gtctggctat cacgaatcac ttctcgcgct tcgtcaagca tctgacgcag
     3121 caattccacc acgcgggtgt gaccataagt atcacgcaaa gaaaggaagg agctatcgcg
     3181 caataagcga tcaatagccg gaagttgact atagagggaa cgcgtttcgg ttgtcatagg
     3241 aaacctggct gatcaaggcc ctctcacacg gagaagggcg tttaacataa ccacggattg
     3301 taacgtgaga tgggtcagga ggacatatcg cgcatcaagc ctttggcggt tcggtgcgag
     3361 caaaactttc gcggctaaac aggttttcga ccagtttgac taagtgtggg cggtcgacac
     3421 accaacctgg cgcaacgcgg cggaaattga gatatccgac ggcacaggca atggcgatag
     3481 tcgccagatt gaccgtatcg gttttgagtg tgccatcgac cagatatcct tccagcacat
     3541 ccagactgcg gttgattttc tcccgctggc gtaacaattc atcttcagac tgctgcgccg
     3601 ctggacgcgc ctgttcacgc accgatacca gcccggcatc cataatgcca tccgccagtg
     3661 cctcaatttt gcgcacccgc aacgactcca gcggatcgcg cggcaacatc gccggagcga
     3721 cattcattaa ttcaatatat tcagcgatga tcggcgaatc aaaccagcat tcgccctctt
     3781 cggtcaccag caccggcact ttccctaacg ggttaaattg cgccacgccg ttgtccgcgt
     3841 tatagggcag ttcattaata aattcgaaag ttatgccctt ttctaacaac agaatagaaa
     3901 gtttgcgtac aaacgggctg gtgtagctac cgacgagttt catgccgagt cctttgtgcg
     3961 aggaaaaata tcagtatggc tcaggctcac gcaaaacaca ggtgatataa gtctcaatga
     4021 tccaaataaa gataatgcat ccagctggtc attcttagca gcacttttcg catcaccgaa
     4081 acatggctga aatggtcgga gtaaaccgcc acctgggcgt agatgccgtc gggtaaggca
     4141 tcgatatcat cgttggggtc cagttcaatg gttcccagca caccgtccgt gccgggcacg
     4201 accgttaatg attgcaatac cccttgcgcc tgatacgaac cgcctggcac gacaggtaaa
     4261 atactggtca gtttgccgtg gaacacctgc ccaggtagcg cgttaaacac cacttccgca
     4321 tcatcgccgg gtttcagacg taacagcgag ttctgccgaa attgggcgac aatttgccgt
     4381 ttttgctcgg ggatgaaaac catcaccgga cgtagcggca aggcagctgc gtatgtacct
     4441 gggcggatca gtacctgagt aacgtagcca ttgcttggtg cgcgaatgac agtctgctca
     4501 aggttatatt ttgcttcagt aagttgcgct cttaagctca caatctgcga ttgctcgccg
     4561 ttaaccatac tgtcgagctg gctctggatc tgcgcctgct ccgccaccga gcctttcacc
     4621 agcgcgtcct gcgcgaggaa gttttgccgc gcatcgtcga tgtcacgttc cgagaacgga
     4681 ttcaccgcca cctggctgcc tttcaggtaa cgttgataat ttttaaacag acggtcgcgc
     4741 tccgctgaaa cctgggtggt gttggcctgc gcttcagtga gctgcgcgcg cagcgtcttt
     4801 atattatgcg tcgccgtcat caggtcagcc tgaagtctgt caactcgcgc ctggtaacga
     4861 accgggtcga gcttaaaaag cacctcgccc ttctgaataa gctgattatt tttgtcggtg
     4921 acttcagtaa caattcccgt cacctgtggc gtgatgggga tcgctatcac tgccttttgc
     4981 gcggtaaaag tgtaagggtg gttgtagttc atcaacaaaa tcaaaccact caccagaaac
     5041 acgcctccca gcgccgccgt cgccagcgtc cactgattca ccggaatgcg gaagatttta
     5101 aagaccgccc acgccagcgc cacgtaagtt aaaacaatca atagatccat atttagatct
     5161 ccgggaacgt ggaatagtca gtctttttct cggcggcgag ttggtgctcc agccgggaaa
     5221 ttcggtcaga aagtgcggcg atttccgggt cagtcgcttt ctcttgcgac gcaacatgtg
     5281 actgcatgcc ccagccgcgc tccggttgat agagcgtcgc ccagatccac agaaacggcc
     5341 agataacatg cagagtaaac aggctcaccc agcccgctgt atgaatagcg tcggcatggg
     5401 ggtggttgcg ctttttggca atcagataag ggatgtcatg tatcgcgatg attccataaa
     5461 aaatcaccag aaagacgaag atcagcactc ccaacgcgaa atagtttaga aacatatctg
     5521 cctcggattc acgtttatca gtgttgtttt ttggctggca gccagaaggg agtcaggctg
     5581 atattttgac aataattcgg gttcgcgatt ctcgccataa caccaaagaa taatttttag
     5641 aggtgatgag ttgcttagtt acataacgat tttatgacga gggcataaca tgctgtagat
     5701 cacatcaggt gaacgccgta agaaaatatc ttgtgattca gatcacaaag attcaacaaa
     5761 ccaacaaaac aaaaatgtga cattactcac atttaaatgc catttttagc gaaaatcgcc
     5821 gccttgttgc ttttttacac aagagttttg tgatgagcat cacgtcaatt acctctctac
     5881 cccctatatt tatgtgaccg atatcacaca aaaggccgtc gactggacag ttaaccgatt
     5941 cagtgccaga tttcgcagta tctacaaggt ccggctacct ctgccgccac attaacaaaa
     6001 aacctcgggc ttccagcctg cgcgacagca aacataagaa ggggtgtttt tatgtcatcc
     6061 gatattaaga tcaaagtgca aagctttggt cgtttcctca gcaacatggt gatgccaaat
     6121 atcggcgcgt ttatcgcgtg gggtatcatc accgcgttat ttattccaac agggtggtta
     6181 ccgaacgaaa cgctggcgaa gctggtcgag ccgatgatca cttatctcct gccgctgctg
     6241 atcggttata ccggtggtaa gctggtaggc ggcgaacgtg gcggcgtagt cggtgccatt
     6301 accaccatgg gcgttatcgt cggcgcagac atgccgatgt tcctcggctc tatgattgca
     6361 ggtccgctgg gcggctggtg cattaagcac ttcgaccgct gggtagacgg taagatcaaa
     6421 tccggttttg agatgctggt gaataacttc tccgcaggca tcatcgggat gatcctcgct
     6481 attctggcat tcctcggcat tggcccgatt gttgaagccc tgtccaaaat gctggctgcg
     6541 ggcgttaact tcatggttgt ccatgacatg ctgccgctgg cgtctatctt tgttgaaccg
     6601 gcgaaaatcc tgttcctcaa caacgccatt aaccacggta tcttctcgcc gctgggcatt
     6661 cagcagtccc atgaactggg taaatccatc ttcttcctga ttgaagctaa cccaggtccg
     6721 ggtatgggcg tgctgctggc gtacatgttc tttggtcgtg gtagcgctaa acagtctgcg
     6781 ggcggtgcgg caatcatcca cttcctgggc ggtatccacg aaatctactt cccgtatgtg
     6841 ctgatgaatc cgcgtctgat tctggccgtt atcctcggtg gtatgactgg cgtgttcacg
     6901 ctgactatcc tgggcggtgg tctggtttct ccggcatctc cgggttctat ccttgctgta
     6961 ctggcgatga caccaaaagg tgcatacttc gctaacatcg cgggtgtgtg tgcggcgatg
     7021 gctgtctcct tcgttgtctc tgccattctg ctgaaaacca gcaaagtgaa agaagaagat
     7081 gatattgaag cagcaactcg tcgtatgcag gacatgaaag ctgagtctaa aggcgcgtct
     7141 ccgctgtctg cgggcgatgt gactaacgac ctgagccacg tacgtaaaat catcgttgcc
     7201 tgtgatgccg gtatgggttc cagtgcgatg ggcgcaggtg tgctgcgtaa gaaaattcag
     7261 gatgcaggtc tgtcgcagat ttcggtcact aacagcgcga tcaacaacct gccgccagat
     7321 gtggacctgg tcatcactca ccgtgacctg accgaacgcg ctatgcgcca ggttccgcag
     7381 gcacagcata tttcgctgac caacttcctc gacagcggcc tgtacaccag cctgaccgaa
     7441 cgtctggttg ctgcccagcg ccatactgaa aacgaagtga aagtgaaaga cagtctgaaa
     7501 gacagctttg acgattccag tgctaacctg ttcaaactgg gcgcggagaa catcttcctc
     7561 ggtcgcaaag cggcaaccaa agaagaagcg attcgttttg ctggcgagca gctggtgaaa
     7621 ggcggttacg ttgagccgga atacgttcag gcgatgctgg atcgtgaaaa actgaccccg
     7681 acttatctgg gtgagtctat cgcggtgcca cacggtacgg ttgaagcaaa agatcgcgta
     7741 ctgaaaacgg gcgtggtgtt ctgccagtac ccggaaggcg tgcgcttcgg tgaagaagaa
     7801 gatgacattg cccgtctggt gattggtatt gctgcccgta acaacgagca cattcaggtt
     7861 atcaccagcc tgaccaatgc actggatgat gaatccgtca tcgagcgtct ggcacacacc
     7921 accagcgtgg atgaagtgct ggaactgctg gcaggtcgta agtaatccaa tcctaccctc
     7981 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     8041 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     8101 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgctg atgtcaatca
     8161 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg tcggtgaaac
     8221 cgagcaggta gataccgttt ctggtgtcga tgccgtcagc agcattggtg atgatgtcgt
     8281 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggtccggttg tgctggaacg
     8341 tattgccccg gcaatcgcca aagggctggt gaaacgtaaa gaacaaggta atgaatcccc
     8401 gctgaacatc atcgcttgtg aaaacatggt ccgcggcacc acacagctga aaggccatgt
     8461 gatgagcgcc ctgccggaag atgccaaagc gtgggtagaa gaacacgttg gctttgttga
     8521 ttccgccgtt gaccgcatcg tgccgccttc ggcttctgca accaacgatc cgctggaagt
     8581 gacggtagaa acattcagcg aatggattgt tgataaaacc cagttcaaag gcgcgctgcc
     8641 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     8701 caccctgaat acgggtcatg ctataaccgc gtacctcgga aaactggcgg gtcatcagac
     8761 cattcgtgat gcgattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     8821 aagtggtgca gtattgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     8881 ccggaaaatc ctcggtcgtt tcgagaaccc gtacctgaaa gatgatgtag agcgcgtagg
     8941 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccac tgctcggaac
     9001 gctggaatat ggtctgccac ataaaaacct gattgaaggt attgccgctg caatgcactt
     9061 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     9121 gctggcggcg ctggcacaga tttccggtct tgatgccaac agcgaggttg tatccgaggc
     9181 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggatac cctgcgcccg
     9241 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     9301 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     9361 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     9421 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     9481 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggaa
     9541 ctgctgatgg cattgcgtga agagctaaat cacgacggca acgagtacgc gtttaccgac
     9601 gacgaaatcc ttggaccctt tggtgaactg cattgcgtgg cggcgttacc accgccgcca
     9661 cagtttgaac ctgcagactc cagtttgtat gcaatgcaaa ttcagcgtta ccaacaggct
     9721 gtgcgatcaa caatggtcct ttcactgaca gagctgattt ccaaaatcag cttaaaaaaa
     9781 gccttccaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     9841 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcagg tattccggac
     9901 ggaatcgcgt tacggggagg tagttttttc agatactccc ggaacgcctg gctcgatgcc
     9961 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
    10021 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt tagatcccgg
    10081 tataaatgac attaaggaaa acgtgctcaa tcctcaataa tcatccgctt ctgttaacta
    10141 tcttttttct ttaaatttct taactaatca atggattggg tgaattttaa acgcattaat
    10201 agttacaatc tggaactatt acaatgccat cagttgtgaa caaagcacct ggtcgcgcat
    10261 actagaggct ataaatttat ctctatcaga agtcatcaca tgaaagaagt cgaaaaaaac
    10321 gaaatcaaac gtctcagcga tcgtctggac gccatccgtc accagcaggc cgatctgtcg
    10381 ctggttgaag ccgcagacaa atatgccgaa ctggaaaaag agaaagccac tctggaagca
    10441 gaaattgctc gcctgcgcga agttcacagc cagaaactca gtaaggaagc acagaagctg
    10501 atgaagatgc cgttccagcg cgcgattact aaaaaagagc aagctgatat gggcaaactg
    10561 aagaagagcg ttcgcggact ggtcgttgtg cacccaatga ccgcgctggg ccgcgaaatg
    10621 ggcctacaag agatgacagg gttctctaag actgcgtttt aagttccttt ctcgtcgctg
    10681 taattacagc cactcacgat ttactcgctc ctgctggcac atgcaggagc ctgttgtaga
    10741 gatcgaatat ttataacgtt cttaacatta tatatttaac tccacctaca cttctgagcg
    10801 cccggagcag gctaaattct gaaaatcagg tacaaattaa agaatgatgt atcaatatta
    10861 acaatgaatt gccgcatatc aatgcttgaa acataattaa ttatttaaat ttttctatca
    10921 gcatcgcaaa attcgcaata aaacatcact caactgaata tttatctttc cctgccccgg
    10981 gcctttatcc ggtgaaacat ttgacattga ttgacaccga tcattaaaat ccgcgcgata
    11041 aatttattta ttggctacaa actaaaattg tttgacactt aaaaacatat tcgcacaacc
    11101 cattaataca aaaattctct aacaaattta tttggcggat atgccccact ctaatcattc
    11161 ataaaaaaaa tgattgtact gtaattatca acgtatcggg attatgaaaa ggaattcatc
    11221 ctatgaacct aaagaaaaca ctgttaagcg cgttaataat agtgcctctc tgcttattgg
    11281 cagggtgtga ctatattgaa aaagcgagta aggtcgacga cctcgttaca cagcaagagt
    11341 tgcaaaaaag caaaattgag gcgcttgaga aacaacagga actcgataag cgaaagatag
    11401 aacattttga aaaacaacaa actactatca taaatagtac caagacgctc gctggcgtgg
    11461 tgcaagcggt taaagacaag caggacgaat ttgtctttac agaatttaac ccagcacaaa
    11521 cacaatattt cattttaaat aacggatcgg tcggtttggc aggccgggtg ctggctattg
    11581 atgcggttga aaatggcagt gttattcgta tttcgctggt taacctttta agcgttcccg
    11641 tatcaaatat tggctttcat gcgacatggg gcaatgaaag acccaccgat gccaaagctc
    11701 tggcaaagtg gcaacaatta cttttcaaca ccaccatgaa ttccaccctg caattaatgc
    11761 ctggtcaatg gcaagacatt aatctgacat taaaaggcgt ttcgcctaat aacctgaagt
    11821 atctgaagtt gtccatcaat atggacaatc tccagttcga cactgttcaa cctgctgaaa
    11881 ctcggcagcg aaaaaacaaa aaataaatgc agcttctgcg cttatatcaa ggaatagaga
    11941 gcatcaataa tgaacaaaat atttaaagtt atctggaatc cggcgacggg cagttacacc
    12001 gttgccagcg aaacggcgaa gagccgtggc aagaaatccg ggcgcagtaa gctgttaatt
    12061 tctgctctgg ttgctggggg aatgttgtcg tcgtttgggg tacaggctca ggcaggaagg
    12121 gacaacggtc agggcgtcaa ttatggccag ggaacaggaa caggaacagg ctgggttgct
    12181 atcggtgaag atgcaaaagc aaatagtttt acagacaccg ggggtggctc cagcaccgca
    12241 gtaggttatc acgcaactgc tgacggaagg tggtcaacag ccctcggcgc taagacccat
    12301 tcgcttggcg aagcatcagt ggcgttaggg attaacacta cctcagcagg tgagcgaagc
    12361 ctcgctatag gtgcatcggc aacgtcaacg ggtggatttt caatcgcact tggtcgttat
    12421 gcaaactcaa caggtgaatt ttctattgca caaggggatt acgcagagac tggtgctgat
    12481 gacgccatcg catttggacg tgaaagcaag gccttaggaa ttatgagtat cgccctcgga
    12541 gccacggcta atgcgtcgaa agaatatgca atggcgctgg gtgcaagtag cgccgcatct
    12601 gcagctaacg cgattgcagt aggccgcaac agtgctgcag ctggcgtaga cagcctcgca
    12661 ttcggcagac aatccgccgc caacgccgca aacgcgattg ctatgggtgc agagagcaaa
    12721 gctgctgaaa atgcgacggc agttggtact aacgcggagg caaatggact taatagtatc
    12781 gcgttgggct ccggcagtat tgcagatgtc gacaacacga ttgctctggg caatcaaagt
    12841 caggcagtag cggctggcgc aattgccatc ggtcaaggga ataaagctga cggcgcaaat
    12901 gctatcgcgc tgggtaatgg tagtattaca ggtggtgtaa atgctattgc tcttggacaa
    12961 ggcagttatg ccggtttaga aaatggcact gcaattggtg ctcaagccag tgctcagggg
    13021 aaaaattcag ttgctctggg tgctggttct gtagcgactg acgcggatac tgtttctgtg
    13081 ggtaacacaa cagctcagcg acaaattgtc aatatggcag caggtgatat cagcactacc
    13141 agtactgatg ccatcaatgg atcacagctt tatgctatca gtaagtcagt agcggacaat
    13201 cttggtgggg gggctaccgt caatgcgcaa ggcgtcgtta cttccccaaa ttacaggctg
    13261 aaaagtggta ttttcggcac tgttggcgac gccttaacgg gcctggacaa taatacgtta
    13321 caatgggact cccttaaaaa ggcatatagt gcggcacatg gtacagatac taccagtacc
    13381 atcaccaacg ttaaagacgg cgctatttct gataccagta aggatgcggt taacggttct
    13441 cagctgaaaa ccaccaacga taacgtagcg accaatactg ccaatatcac caccaacact
    13501 aacagtatca ataccctgac ggattccgtt ggcgacctta aagacgatgc cctgctgtgg
    13561 aatggcaccg cgttcagcgc cgcgcacggc accgatgcca ccagcaaaat caccaacgtc
    13621 aaagacggtg acctgacggc tggcagcacc gacgcggtta acggctctca gctgaaaacc
    13681 actaacgatg ccgtggcagc caacaccacc aatatcgcca cgaataccac caacatcacc
    13741 aacctgactg acgcagttga cagcctcggt gacgattccc tgctgtggaa cgctacagcg
    13801 ggggcattca gcgccgcaca cggtactgat gccaccagca aaatcactaa cgtcaaagac
    13861 ggtgacctga cggctggcag cactgacgcg gttaacggct ctcagctcaa aaccactaac
    13921 gatgccgtgg cagccaacac caccaatatc gccacgaaca ccactaacat caccaacctg
    13981 actgacgctg ttgacagcct cggtgacgat tccctgctgt ggaacgctac agcgggggca
    14041 ttcagcgccg cacacggtac tgatgccacc agcaaaatca ccaacgtcac cgctggcgac
    14101 ctgacggctg gcagcaccga cgcggttaac ggctctcagc t
//
LOCUS       DAPOZO010000115        13237 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPOZO010000115 DAPOZO010000000
VERSION     DAPOZO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.115"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            18..2477
                     /locus_tag="ROI21_003981"
     CDS             18..2477
                     /locus_tag="ROI21_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDX5112848.1"
                     /translation="MDTVNIYRLSFISCLVMAIPSALAVEFNLNVLDKSMRDRIDISL
                     LKEKGGIAPGEYFVSVAVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDNDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVADNVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQLPPLGADIRQEDSGISVGMVGEEGHAWLSGVAENQQFTVIWGDSQR
                     CSIHLPEHMEDTANRLILPCH"
     gene            2493..3224
                     /locus_tag="ROI21_003982"
     CDS             2493..3224
                     /locus_tag="ROI21_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDX5112849.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKNDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLNLKNPTGYYLTIAYLGRNEQGVLPGFKTVMVAPFSTVITNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCNGHCRLQAVEAKK"
     gene            3221..4282
                     /locus_tag="ROI21_003983"
     CDS             3221..4282
                     /locus_tag="ROI21_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDX5112850.1"
                     /translation="MSTGKGLLLVICLLFLPLKSALALNCYFGTSGGTVEKSEAIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREIPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLIEEDKALLIGNGLKLRLLDENSSPYTFNKYSEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            4435..5481
                     /locus_tag="ROI21_003984"
     CDS             4435..5481
                     /locus_tag="ROI21_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDX5112851.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLDDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(5478..6269)
                     /gene="nei"
                     /locus_tag="ROI21_003985"
     CDS             complement(5478..6269)
                     /gene="nei"
                     /locus_tag="ROI21_003985"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDX5112852.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVETGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLSAAQLDALADALLDIPRLSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6305..7039)
                     /gene="pxpA"
                     /locus_tag="ROI21_003986"
     CDS             complement(6305..7039)
                     /gene="pxpA"
                     /locus_tag="ROI21_003986"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDX5112853.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGNAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALEQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(7029..7961)
                     /gene="pxpC"
                     /locus_tag="ROI21_003987"
     CDS             complement(7029..7961)
                     /gene="pxpC"
                     /locus_tag="ROI21_003987"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDX5112854.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPMGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(7955..8611)
                     /gene="pxpB"
                     /locus_tag="ROI21_003988"
     CDS             complement(7955..8611)
                     /gene="pxpB"
                     /locus_tag="ROI21_003988"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDX5112855.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGIYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(8634..9377)
                     /gene="ybgI"
                     /locus_tag="ROI21_003989"
     CDS             complement(8634..9377)
                     /gene="ybgI"
                     /locus_tag="ROI21_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDX5112856.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            9648..11129
                     /gene="dtpD"
                     /locus_tag="ROI21_003990"
     CDS             9648..11129
                     /gene="dtpD"
                     /locus_tag="ROI21_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDX5112857.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRLAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILILSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            complement(11172..12590)
                     /gene="phrB"
                     /locus_tag="ROI21_003991"
     CDS             complement(11172..12590)
                     /gene="phrB"
                     /locus_tag="ROI21_003991"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDX5112858.1"
                     /translation="MTTHLVWFRQDLRLHDNFALAAACRYSSARVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLQIALAEKGIPLLFHEVDDFAASVEIVEQVCAENRVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFQNAWLK
                     RLREGMPECVAAPKVRSSGMIEPAPSITLNYPRQPFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(12587..13096)
                     /locus_tag="ROI21_003992"
     CDS             complement(12587..13096)
                     /locus_tag="ROI21_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDX5112859.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALNDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMAFYPDAWLSEQRYFELWPRV
                     INLRHAGVL"
BASE COUNT         3163 a         3564 c         3251 g         3259 t
ORIGIN      
        1 tcaaatattt atcaggtatg gacaccgtga atatttatcg actctctttt atatcctgcc
       61 tggtcatggc gataccttcc gcattggcgg tcgaattcaa cctgaatgtc ctcgataaat
      121 cgatgcgaga tcgcattgat atttcattat taaaggaaaa agggggcatt gctcccggtg
      181 aatattttgt tagcgttgca gtaaataaca accaaatcag taatggacaa aaaattgact
      241 ggaaaaagaa cggtgaccaa accattccgt gtattaatga tttactggta gataaatttg
      301 gtttaaaacc tgaagttcgt cagtcgttac cacggttaaa ccagtgcgtc gattttagct
      361 cccggcctga aattctcttc atttttgatc aggctagcca gcaactgaat atcactattc
      421 cacaagcctg gctggcgtgg cactcagata actggactcc cccctcaaca tggaaagaag
      481 gtgtcgccgg cgtcctgatg gactacaacc tgtttgccag cagctaccgc ccacaggatg
      541 gtagcaacag cactaacctg aacgcctatg gaacagccgg aattaacgcg ggagcatggc
      601 gtttacgcag tgattaccaa ttgaatcaga ccgatagcga tgataaccat gaacagtcag
      661 gcgaaatatc acgcacctat ctttttcgtc cattaccgca attaggttca aaattaaccc
      721 ttggtgaaac ggatttcagt tccaatattt tcgacggctt ttcttatacc ggcgcggcac
      781 tggcaagtga cgatcggatg ttaccgtggg agctacgcgg ctacgcccca caaatcagcg
      841 gcattgcaca aaccaacgcc accgtcaccg tgagtcactc agggcgagtt atttaccaga
      901 aaaaagtccc accaggtccg tttatcattg atgaccttaa tcagtccgtc cagggtacgt
      961 tggatgtcaa agtgacggaa gaagatggtc gggtgaacaa tttccaggtt tcggcagcat
     1021 cgactccctt cctgactcgc cagggacagg ttcgctataa attggccgcg ggtcagccac
     1081 ggccctccat gtctcatcaa actgaaaatg aaaccttttt tagcaatgaa gtttcctggg
     1141 ggatgctgtc aaatacctca ctgtacggcg gcctgctgat ttcgggtgac gactaccatt
     1201 cagccgcaat gggtatcggg caaaatatgc tctggcttgg tgcattgtcg tttgatgtca
     1261 cctgggccag tagtcagttt gatactcagc aggacgagcg gggcttaagt taccgcttta
     1321 attacagcaa acaagtggat gccactaaca gcacgatttc gctcgccgct tatcgcttct
     1381 cagatcgtca ttttcacagc tacgccaact atctggatca caaatacaac gacaacgatg
     1441 cgcaggacga aaaacagacg atcagcttat ccgtgggcca accgattacc ccactaaacc
     1501 tcaatcttta cgccaacctg ctgcatcaaa cctggtggaa tgcagacgcc tccacgaccg
     1561 ccaacatcac ggcgggtttt aatgttgata ttggtaactg gagagatatc tcgatctcga
     1621 catcattcaa tacaacccat tacgaagata aagatcgcga caatcagatt tacctgtcga
     1681 tttcgctccc cttcggtaat ggggggcggg tcggttatga catgcaaaac agtagtcaca
     1741 gcaccacgca ccgcatgtcg tggaatgata cgttagatga acgtaatagc tggggcatgt
     1801 ctaccggact gcaatccgac cgtccggata atggagccca ggtgagcggt aactatcagc
     1861 acctgagttc agcgggtgag tgggatattt ctggtaccta tgccgccaat gattacagtt
     1921 ccgtcagcag cagctggagc ggttctttca cagcaaccca atatggtgcg gcgttccatc
     1981 gtcgcagctc caccaatgaa ccacgcctga tggtcagcac cgatggcgtg gcagatattc
     2041 cggttcaggg caatctcgac tacaccaacc attttggcat tgcggtggtg ccgttgattt
     2101 ccagttacca gccttccacc gtggcggtga acatgaatga tttacccgac ggcgtaacag
     2161 tggcagataa cgttatcaaa gaaacgtgga ttgaaggcgc gataggttac aaatcgctgg
     2221 cttcccgttc cggtaaagac gttaacgtca tcattcgcaa cgccagcggt cagctccccc
     2281 ccctcggtgc ggatatccgc caggaggata gcggcattag cgtgggaatg gttggcgagg
     2341 aaggacacgc ctggttaagt ggagtcgctg aaaatcaaca atttaccgtg atctggggtg
     2401 atagccagcg ttgctcgatc catcttcctg aacatatgga agacaccgca aatcgcctga
     2461 ttttaccttg tcattaatta aggaaatgaa aaatgacatt tatgaaagga ctaccccttt
     2521 tgttgttggt tgccagtctg tgcagccacg ctgcactaca acccgatcgc actcgtattg
     2581 tgtttaacgc taatgataaa gctaccagtc tgcgcgtgga taatcgtagc gataaactcc
     2641 cctacctcgc ttattcctgg ctcgaaaatg aaaaagggga gaaaaacgat gatcttctgg
     2701 ttgccctgcc accgatacaa cgtctggaac cgaaagcaac aacgcaggta cggatagtaa
     2761 agcaagcctc gaccacaaaa ttaccgggcg atcgcgaaac gctatttttc tacaatatgc
     2821 gtgaaattcc tcccgccccg gaaaaaaata gcgaccatgc cgttctgcaa gtcgccattc
     2881 agagtcgtat caaggtattc tggcgaccgg ctgcattacg caagaaagca ggagaaaagg
     2941 tcgaactgca gttacaggtc agccagcagg gcaatcaact taacttaaaa aacccgaccg
     3001 gctattacct gacaatcgcc tacctgggac gaaatgagca aggtgttctc ccgggcttca
     3061 aaaccgtgat ggttgcgcct tttagtacag taattacaaa caccggaagt tatagcggca
     3121 gtcagtttta tctcggttat atggatgatt acggcgcgtt acgcatgacg acgctcaatt
     3181 gcaacggaca ttgccgttta caagcagtgg aggcgaagaa atgagtactg gcaaaggatt
     3241 gttgctcgtc atctgtctgt tatttctgcc attgaagtct gcgctagcgc tgaactgcta
     3301 ttttggtact tccggcggaa cggtagaaaa atcggaagcg attcaaccct ttgctgtgcc
     3361 aggtaatgcc aaacccggcg ataagatctg ggaatctgac gatattaaaa ttcccgtcta
     3421 ttgtgacaac aataccaacg gcaactttga aagcgaacat gtctacgcat gggtaaaccc
     3481 ctacccgggg gtacaggatc gttattatca actcggcgtg acatacaatg gtgtcgatta
     3541 cgatgccagt ctggggaaaa gccgcatcga caccaaccag tgtatcgaca gtaagaacat
     3601 taacatttat acccctgagc agattatcgc gatgggctgg cagaacaaaa tatgctcggg
     3661 tgaccccgct aatattcaca tgtcgcgaac ctttcttgct cgtatgcggt tatacgttaa
     3721 gattcgcgaa ataccgccgc atgattatca aagtacgctt agcgactaca tcgtcgtgca
     3781 atttgatggt gccggtagcg ttaacgaaga ccctaccgcc caaaacctga aataccatat
     3841 tactggtctg gaaaacattc gtgtactgga ttgcagcgtc aatttttcta tttcaccaga
     3901 aacacaggtg attgattttg gtaaatttaa cctgctggat attcgccgcc acacaatgtc
     3961 gaaaacattc agtattaaga cgacaaaatc acaaaatgac cagtgcaccg acggatttaa
     4021 ggtcagctct tcgttttata ccgaagaaac attgattgaa gaggataaag cactgctgat
     4081 tggtaatgga ctgaagctac gtttgctgga tgaaaattct tcaccctata ctttcaacaa
     4141 atacagcgaa tatgctgatt tcaccagcga tatgctgatc tacgaaaaaa cctatacggc
     4201 tgaactttcg tccattcccg gcacccccat cgaagcgggt cccttcgata cagtggtgct
     4261 ttttaagatt aactataact aatttatcat gatgcccttt gcagtcagta aagggcctct
     4321 ctttcagaaa attgctgatt ttcccctttt ccttgattgc tgttattttt tcctggcccg
     4381 gctttcttta tgctgtatcg atacaccaaa gaaaaaataa tgagggagcg aaggatgcca
     4441 gttttgcagt ggggaatgtt atgtgtgctc tcacttctcc tttctattgg cttcctcgga
     4501 ttacatctcc cggcggcgct attacttgga ccgatgatcg ccgggatcat ctttagtatg
     4561 cgcggaatta ctttacaact cccccgctcc gcttttctcg ccgcccaggc cattcttggc
     4621 tgcatgattg cgcaaaacct cactggttcg attctcacca ccctggccgt caactggccg
     4681 atcgtgcttg caattttact ggtaacgctg ctttccagcg ccatcgtggg ttggttattg
     4741 gtgcgctata gctcattgcc tggaaatact ggtgcctggg gttcttctcc tggcggcgcg
     4801 gcggcaatgg tcgctatggc gcaagattac ggcgcagata ttcgcctggt ggcgtttatg
     4861 caatatctgc gagtgctgtt cgtcgcgggg gccgctgttc tggtcacccg catgatgctg
     4921 gatgataacg ctgaagcggt taaccagcag atcgtctggt tcccgccagt gagcattaat
     4981 ctcctgctta cgattttgct cgcagtcgtt gccggtacgg cgggatgctt gctacgtctt
     5041 ccttccggca cgatgctcat cccgatgctg gcgggcgcag tgctccagtc tggtcagctc
     5101 atcaccatcg aactaccgga atggctgctg gcgatggcgt atatggcaat tggctggcgg
     5161 attggtcttg gtttcgataa acaaatctta ctgcgggcat tgcgcccgct accgcaaatc
     5221 ctgctgtcga tttttgctct gctggctatt tgtgcgggta tggcgtgggg actgacccga
     5281 tttatgcaca ttgattttat gactgcctac ctcgccacca gccccggcgg gcttgatacc
     5341 gtggcggtca tcgccgcagg gagcaatgcc gatatggcgc tcattatggc gatgcaaacc
     5401 ctgcgcctgt tcagtatttt gctgacgggg ccagccattg cgcggtttat ttcaacctat
     5461 gcgccgaaac ggtcggccta gtgctggcag cccggacacc agtaaaacgg gcgagatgac
     5521 agcgtggttt tctcaatgat gctgccacag cgttcacacg gttcgccatc acgatgaaaa
     5581 accttaaagc gaaacaacgc cccatgatgt ttattctcat ccacctgccc ccgcgtagcg
     5641 taagaaagtc gaggaatatc cagtaacgcg tctgcgagtg catccagttg cgccgcactg
     5701 agatctttcg ctttatgatt gccggttaac cccacctgcc agaggatctc cacccgcaaa
     5761 taattgccaa gtcctgccag aaacgcctga tcgagcagta atccagcaaa ctgacggtta
     5821 cgaaagcgcg gcgacaataa tcgttccttt accacctctg gcgtcagatt cggatccagc
     5881 acatctggac caacgcgttg taaaaacgga tgcgtggtca gttgttccgg ggtcaacatc
     5941 tcaatatccg aggcgctata aagcagaatg gttttgtcag ccgtttgcag ttttacccgc
     6001 aatactcgcg tggtctgcgg ctcttcgccg gtttcaacca cgcgccagac gccgtaaagc
     6061 tgattatggc tgtagagcgt taagtcgttg gaaaaatgag ttaacaacgc cttaccacgc
     6121 gtttccacat gggtaacgtg ttgaccgata agtcgtgatt gataagattt taactgcggg
     6181 aaggcaaacc agacatcagt tagtggtttg cctttgatcg ccgcctccag gttatccgct
     6241 gcacggcgga tctccgggcc ttcaggcatg gtgatatcct tttttcattc tttattaagc
     6301 ggggttatgc tgcgacaaca atcccctttt cggcaaatgt agagcgtagt cggcgggcga
     6361 acgccagtgc gtgctcgcca tctccatgca ggcagaccgt ttgcgccgtg accgttgccc
     6421 attcgccggt gatgcttttg actctgccgt gttgcaccat ctccaacgtt tgctccagcg
     6481 cctgttcttc gttttcaatc aacgcgcctg gctggcttcg cggcaccagc gaaccgtctg
     6541 cctgataacc gcgatcggca aacacttcct cgcgcgttgt caggccatat tgcttgcctg
     6601 cacgaatcag ctcgcttcct gccagcccga cgagaatcaa tgccggatcg caagcgtata
     6661 ccgctctggc gatggcgtct gccagttgtg cctctttcgc cgcctggttg tataacatgc
     6721 cgtgcggttt gacatgacac attacgccgc cttgcgcacg ggtaatcgct gccagcgcgc
     6781 caatttgata cagcgtctgg gcgtaaacgg tttctggcgg cagctgcatg gcgctgcgac
     6841 caaaattttc cctgtcggga aaactcgggt gagcgccaat cgcgacacca ttttttattg
     6901 cttcacgcac gcaagcctgc atggtttgcg cattgcctgc atgaaatcca caagcaatat
     6961 tggcagagga aaccagcgtt aatagctctg cgtcgctggc gcagccttcg cccagatcgg
     7021 cgttcaggtc aattttcatt gtgcagccgc cacgctaatt gttcgaaata acgttgctga
     7081 tcttgccgtg ctttcagtgc ctcttccagt gaacactgga caaaatgaat cggctgaccg
     7141 agcggaattt gcgccagatg gtacatatca gcctcaatga tacaggcaat acgcggataa
     7201 ccaccggtgg tctgtgcgtc gttcatcaat acaatcggct ggccgttatg cggcacttgc
     7261 accacacccg gcaacaaacc gtgagataac agttcgcgat cggtggtgcg ttttaaaatt
     7321 tgcccctgta agcgatagcc catgcggtta ctttgcggac taagctgcca gggcgaacgc
     7381 cagaatgcat cctgcgaggc gcgatcgaac tcatgatatt ccggccccgg caaggcgcga
     7441 atgcggttgc cccacagcaa ctgtttaacg ccctgcgctt ccataaaatc acgcttcgat
     7501 ttacccatcg ggagtcggtc accatccttc agtaaacggc cttccagccc accaatcccc
     7561 actttgagat cggtactgca tgaccccatt accggcggaa catcaatacc acccgcgacc
     7621 gccagataac tgcgcatccc gtgctgcggg cgtttaagcg ttaaacgctg gcctgctttc
     7681 atcggcaatc gccagccggt ccagacggcg ttatcatcca gccgcgcttc gcaaccggcc
     7741 cccgtcagag caaaccaccc atcagtttcg aactcaaccg ttaactgacc gagcgtgatc
     7801 tccagcgcgg gggcattggc gtcattaccc accagtaggt tagcaatgcg taacgcgggc
     7861 atatccagtg cgccgcagtg gctgataccc gactggcgaa aaccgtgacg accgccatcc
     7921 tgcacagtgg tgtacatgcc cgcacgaata atcttcagca tactccctcc ttctgcggta
     7981 caaagcgcac gctgtctccc ggtcgtaata agatgggttc gtcacgcgcc ggatcaaaca
     8041 ggctgagtga ggtatgacca atcaactgcc agccacccgg cgttgccagc ggataaatac
     8101 cagtctgcgg cccgccgatc ccgacagaac ctgccggaac gagcaagcgc ggttcagcgc
     8161 gccgtggcgt atgtagttgt tccggcaaac tcccgagata cgggaagccc ggttgaaaac
     8221 ctaaaaacca gaccacgtat tccacggagg agtgcaattc aacaacctgt ttttcgctca
     8281 atccacaatg cgccgcgacc accgccaaat ccggtccggc tgcgccaccg taaaccaccg
     8341 gaatttcaat aaagcgagac tccggctcca gcgcctcact ctcctcccac cagcgttgca
     8401 aacgctctat ggcatccagc gccagcgact caggattacg caaaatcacc gtgatattgt
     8461 tcatgccggg aatggcttca accacattcg gcatatccac cagacgctgt gccagtcgcc
     8521 agatccgttt ctggctagcc agcgtcaccg gcggttccag ctccagcact accgccgttt
     8581 cacctatcag ataacaacgc gctcgttgca ctttcgatcc ctctgattat tcgttatgca
     8641 ggattaggaa tatcaataaa ggtcacatca agatcggtat tttcattcag ccactcgctc
     8701 aatgcgcgaa taccaccgcg ttcagtggca tggtgacccg cagcataaaa atgcaatccc
     8761 tgctcgcggg ctgaatgaat ggtctgttca gaaacttcgc cagtaataaa agcatccacg
     8821 ccaaaacgcg cggcgctatc gataaaactc tgcccgccgc ccgtgcacca ggcaacgcgc
     8881 tggaccacct caggtccggt atcgccacac cataacggct tacgtcccag acgcgcttca
     8941 atccaggaag ccagctccag tcccggcaca ggcatggtca gttcgcccca cggcaccaac
     9001 ggctcaattt cgcccatgac cgtgatcccc agtaacgccg ccagctgtgc gttattgccc
     9061 agctcaggat gtgcgtcaag cggcaaatgc cagccataca ggttgatatc atttgccagc
     9121 aacgttttta aacggttacg cttcattccg cgaatgaccg gagattcgcc tttccagaag
     9181 tagccatgat gcacaatgac cgcatccgcg cccagacgca ctgcctcatc gagcaaagcc
     9241 tggctggcag tgacaccggt aacaattttt tgcaccgtct ctttgccttc cacctgcaaa
     9301 ccattcggcg cgtaatcact aatcgccgcg ctgttcagtt tttcgttaat cagttgttcc
     9361 agttcggtgt ttttcatctt cgcgccttcc gtattctgat agctacaccg ttaacatagc
     9421 gttacctcgc ctgttcgtcg atagccaatc ttttgacata ttaagaaaca aacccgacgc
     9481 caaaagacgc aaattgttta tatcatcacg cttcagctaa tgtatattta tgcaatcaga
     9541 cagcatattg cataaataat cagatctgta tcaaagtttt aactttaagg aaacacaagc
     9601 acgccgcgca actattacgt gacgttgcag tcaattgtgg aattattatg aataaacacg
     9661 catcacagcc gcgcgctatt tactatgtcg ttgcgctgca aatctgggaa tattttagct
     9721 tttacggcat gcgtgccctg ctgattctct atctcaccaa tcaactaaaa tacaacgata
     9781 ctcacgccta cgagttattt agcgcctact gttcgctggt gtatgtcacg ccaatcctcg
     9841 gtggcttttt ggcggataaa gttctcggca atcgcctggc ggtgatgctg ggggcgttat
     9901 tgatggcgat cggtcatgtg gtgctgggtg ccagtgagat acatccgtca ttcctctatc
     9961 tgtccctggc gattatcgtt tgtggctatg gtctgtttaa atccaacgtc agttgcctac
    10021 tcggcgagct gtatgagcca accgatccgc gtcgtgatgg cggtttctcg ctgatgtatg
    10081 cggcgggtaa cgtggggtct attatcgcac ctatcgcctg tggctacgcc caggaagagt
    10141 acagttgggc gatgggcttt ggcctggcgg cggttggcat gatcgcgggt ctggtcattt
    10201 tcttatgtgg caatcgtcat ttcactcata cccgcggcgt taacaaaaaa gtactgcgtg
    10261 cgacaaactt tctcctgccg aactggggat ggctgctggt tctgctggtg gcaacgcctg
    10321 cgctgatcac cgtactgttc tggaaagagt ggtcggtata cgccttgatt gtcgcgacta
    10381 ttattggcct gggtgtatta gcaaaaattt atcgcaaagc agaaaaccag aaacagcgta
    10441 aggagctggg gctgattgtt accctcacct tcttcagcat gttgttctgg gccttcgcac
    10501 aacagggcgg cagctcgatt agcctttata tcgaccgctt cgttaaccgc gatatgtttg
    10561 gttataccgt tccgactgct atgttccagt cgattaatgc cttcgcagtt atgctgtgcg
    10621 gagtgttcct ggcgtgggtg gttaaagaga gcgtcgcagg taatcgtacc gtgcgcatct
    10681 ggggaaaatt tgctcttggc cttggcctga tgagcgccgg attctgcatt ctgatcttaa
    10741 gcgcccgctg gtccgcaatg tatggtcact cttctctgcc actaatggta ttaggcctgg
    10801 cggtgatggg ctttgcggaa ctgtttatcg acccggttgc tatgtcgcag attacgcgca
    10861 ttgaaatccc tggcgtgact ggcgtattaa ctggcattta tatgctgctt tccggcgcga
    10921 ttgcgaacta tctggctggc gtgattgccg atcagacatc gcaggcttcg tttgatgctt
    10981 ccggggcgat caactattcc atcaatgcat atattgaagt atttgatcaa attacctggg
    11041 gcgcactggc gtgtgtagga gtggtgttga tgatttggct gtatcaggcg ctgaaattca
    11101 gaaaccgcgc gctggcgctg gagtcttaac aggccagcac cgatgcctga taaaacgctc
    11161 gctgcgtttt atcaggctcc tttcctcgct tcttcgtacg ctgccagcgt tcgcagcctt
    11221 gcctctttgt gatcaactat cggttgcgga taatccagcg tcacacctgc tttctgcgcc
    11281 cacttccacg gctcatgcac cactttccct ggcacatcgc gcagttccgg tagccactgg
    11341 cggataaact cgccttcgcg gtcaaatttc tcgccctggg ttgtcgggtt aaaaatacga
    11401 aaatacggcg ctgcatcggt tccggttgaa gcggcccact gccagccacc gttattagct
    11461 gccaaatcac catcaatcag ctgcgacatg aaatatcgct cgccttcgcg ccagtcgatc
    11521 aacaaatctt tcaccagaaa actggctgta atcatccgta gcctgttatg catccagcca
    11581 gtgctgttaa gctgacgcat ggcagcatcg acaatcgggt atcccgtttt gccttcctgc
    11641 caggcctgta aatgtgcggg attgctctgc cactgcacac gatccgtcca ggcaataaac
    11701 ggacaatgtt tacacaacga ggggtaatac gtcatcagat gacggtagaa ctcgcgccag
    11761 atcagctcac taagccagac actaccggcc ccaccgtcca gcgcctgcgg ctgttccgcc
    11821 aacaggcgat gcagacactg gcgaggcgat aacccgcccg ttgccaggct ggcggacaaa
    11881 cggctggtac cttcaacagc cggaaaatct ctttgttgct catactctcc ggcaccgttc
    11941 tggcaaaact ggcgtaattg cgcaatcgcc gctttttctt ccaccgggaa atgcgcagta
    12001 tcgaaaggct gacgaggata attcagcgta atggatggcg cgggttctat cattccgcta
    12061 ctacgtactt ttggcgcagc gacgcactcc ggcatccctt ctcgcagccg tttcagccag
    12121 gcattttgaa aaggcgtaaa gactttgtac atctcatgat tgccagtcat caccgcgcca
    12181 ggcggcagga tcacgctgtc atcaaatcct tcacacacca cgttacgcag cgttctctca
    12241 acctgcacat cccgcgcccg ctcattcacc tcatactgat agttataaaa cagatgggta
    12301 acacgatttt ccgcgcacac ctgttcaact atttcgacgc tggcggcaaa gtcatccact
    12361 tcatgaaaca gcaaaggaat gcctttttcc gccagcgcga tttgtagccc gttcagttgt
    12421 gcattgataa attcagcctg acgcggcgac atattatgcg ccgcccactg gcgtggtgta
    12481 gcgatataca acgccagcac gcgtgcagac gaatagcggc aagcggcagc cagtgcgaaa
    12541 ttatcgtgca gacgtaaatc ctggcgaaac cagaccagat gggtagtcat aaaactcctg
    12601 catggcgcaa gttaatcaca cgcggccaaa gttcgaaata acgctgctct gaaagccagg
    12661 cgtcaggata aaacgccata tagtgtttga tacgcatcag cggcgcaaga agcggttgct
    12721 cgccacggcg ataactgtcg ataagcgccg ccagctgctg gcgttctgtg gaatcaatat
    12781 gtgggcgaaa atatccctga acgtgcatca aaacgttagt atgatcacgc cggttggcag
    12841 ggtgagaaag caaaacaatt acgcgttggc ggtactggtt gtaatagtca tccagatttt
    12901 gccactggtg aatatcagcc acaaacgggc caagtttgcg gtactcgggc tgggaatggg
    12961 ccagaaagac gagtttatag cggctgtgat aatcgttgag tgccccgcgc gttaagccat
    13021 gttctttcag tcgatgtaac tcatgaaggg cgaagatacg gattagggtg ctgtcatcaa
    13081 atcgttgctg attcattact gctcctgaac ggtttacagg agtaagtgta gacgttggga
    13141 aatgatattt gtgaagttaa tcctgttgta gcagggcaag attacgaaag cccgctcccc
    13201 gcaaggactg acgtcaggta gtttctgtcc atggctg
//
LOCUS       DAPOZO010000116        13148 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPOZO010000116 DAPOZO010000000
VERSION     DAPOZO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.116"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            89..321
                     /locus_tag="ROI21_003993"
                     /pseudo
     CDS             89..321
                     /locus_tag="ROI21_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(401..1873)
                     /locus_tag="ROI21_003994"
     CDS             complement(401..1873)
                     /locus_tag="ROI21_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDX5112860.1"
                     /translation="MNCSLTLSQRLSLVFTVVLLFCAAVTCGVHIYSSNLYGNAMVQR
                     LSAGLAQQIVITEPLLDNRGQVNHRTLKSLFERLMTLNPSVELYIVSPEGRLLVEAAP
                     LGHIKRRYINIAPLKKFLSGAVWPVYGDDPRSVNKKKVFSTAPLYLRDDLKGYLYIIL
                     QGEELNALTDAAWTKALWNALYWSLFLVVICGLLSGMLVWYWVTRPIQQLTENVSGIE
                     QDSISAIKQLAIQRPATPPSNEVEILHNAFIELARKISCQWDQLSESDQQRREFIANI
                     SHDLRTPLTSLLGYLETLSMKSDSLSSEDCHKYLTTALRQGHKVRHLSCQLFELARLE
                     HGAIKPQLEQFSVCELIQDVAQKFELSIETRRLQLRIMMSHSLPLIRADISMIERVIT
                     NLLDNAVRHTPPEGSIRLKVWQEDNRLHVEVADSGPGLTEDMRTHLFRRASVLCHEPS
                     EEPRGGLGLLIVRRMLVLHGGDIRLTDSTTGACFRFFLPL"
     gene            complement(1874..2593)
                     /locus_tag="ROI21_003995"
     CDS             complement(1874..2593)
                     /locus_tag="ROI21_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDX5112861.1"
                     /translation="MNNVKKILLMEDDYDIAALLRLNLQDEGYQIVHEADGARARLLL
                     DKQTWDAVILDLMLPNVNGLEICRYIRQMTRYLPVIIISARTSETHRVLGLEMGADDY
                     LPKPFSIPELIARIKALFRRQEAMGQNILLAGGLICCHDLCINPFSREVHLHNKQVDL
                     TPREFDLLLWFARHPGEVFSRLSLLDNVWGYQHEGYEHTVNTHINRLRAKIEQDAAEP
                     KMIQTVWGKGYRFSVDNAGMR"
     gene            2734..3332
                     /locus_tag="ROI21_003996"
                     /pseudo
     CDS             2734..3332
                     /locus_tag="ROI21_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
     gene            3332..4075
                     /locus_tag="ROI21_003997"
     CDS             3332..4075
                     /locus_tag="ROI21_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDX5112862.1"
                     /translation="MKKKHFLSDSDAHYVVKDAEKILAPDRRRLLQQGLTLGGIMMLT
                     GCDISNNDDIEKALFRISRFNDRIQSFLFSKNSVAPEYLPEDITTPFPFNAFYGEEEI
                     PVIDGQDYKLEIRGLVRDNRNWFLSELYNMQHVSQITRHICVEGWSAIGKWGGIPFRH
                     FLTLIGADMRAKYVSFRCVDGYYTSIDMATALHSQTLLTLTWNNRVLPKEYGYPIKIR
                     IPTKLGYKNPKHVQVIEVTNHFPGGYWED"
     gene            4130..4372
                     /locus_tag="ROI21_003998"
     CDS             4130..4372
                     /locus_tag="ROI21_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112863.1"
                     /translation="MKNIISVTVLSALFMFNAQAADTMDKTHEVKGSMSSAHDKMCKD
                     GMCKNAMDKEKMEKEHMSKTDGMKNDHMGKKDNMSQ"
     gene            5555..6067
                     /gene="hlyC"
                     /locus_tag="ROI21_003999"
     CDS             5555..6067
                     /gene="hlyC"
                     /locus_tag="ROI21_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin-activating
                     lysine-acyltransferase HlyC"
                     /protein_id="HDX5112864.1"
                     /translation="MNMNNPLEVLGHVSWLWASSPLHRNWPVSLFAINVLPAIRANQY
                     ALLTRDNYPVAYCSWANLSLENEIKYLNDVTSLVAEDWTSGDRKWFIDWIAPFGDNGA
                     LYKYMRKKFPDELFRAIRVDPKTHVGKVSEFHGGKIDKQLANKIFKQYYHELITEVKN
                     KTDFNFSLTG"
     gene            6079..9153
                     /gene="hlyA"
                     /locus_tag="ROI21_004000"
     CDS             6079..9153
                     /gene="hlyA"
                     /locus_tag="ROI21_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin hemolysin HlyA"
                     /protein_id="HDX5112865.1"
                     /translation="MPTITTAQIKSTLQSAKQSSANKLHSAGQSTKDALKKAAEQTRN
                     AGNRLILLIPKDYKGQGSSLNDLVRTADELGIEVQYDEKNGTAITKQVFGTAEKLIGL
                     TERGVTIFAPQLDKLLQKYQKAGNKLGGSAENIGDNLGKAGSVLSTFQNFLGTALSSM
                     KIDELIKRQKSGSNVSSSELAKASIELINQLVDTAASINNNVNSFSQQLNKLGSVLSN
                     TKHLNGVGNKLQNLPNLDNIGAGLDTVSGILSAISASFILSNADADTGTKAAAGVELT
                     TKVLGNVGKGISQYIIAQRAAQGLSTSAAAAGLIASVVTLAISPLSFLSIADKFKRAN
                     KIEEYSQRFKKLGYDGDSLLAAFHKETGAIDASLTTISTVLASVSSGISAAATTSLVG
                     APVSALVGAVTGIISGILEASKQAMFEHVASKMADVIAEWEKKHGKNYFENGYDARHA
                     AFLEDNFKILSQYNKEYSVERSVLITQQHWDTLIGELAGVTRNGDKTLSGKSYIDYYE
                     EGKRLEKKPDEFQKQVFDPLKGNIDLSDSKSSTLLKFVTPLLTPGEEIRERRQSGKYE
                     YITELLVKGVDKWTVKGVQDKGSVYDYSNLIQHASVGNNQYREIRIESHLGDGDDKVF
                     LSAGSANIYAGKGHDVVYYDKTDTGYLTIDGTKATEAGNYTVTRVLGGDVKILQEVVK
                     EQEVSVGKRTEKTQYRSYEFTHINGKNLTETDNLYSVEELIGTTRADKFFGSKFTDIF
                     HGADGDDHIEGNDGNDRLYGDKGNDTLRGGNGDDQLYGGDGNDKLIGGTGNNYLNGGD
                     GDDELQVQGNSLAKNVLSGGKGNDKLYGSEGADLLDGGEGNDLLKGGYGNDIYRYLSG
                     YGHHIIDDDGGKDDKLSLADIDFRDVAFRREDNDLIMYKAEGNVLSIGHKNGITFRNW
                     FEKESGDISNHQIEQIFDKDGRVITPDSLKKALEYQQSNNKASYVYGNDALAYGSQDN
                     LNPLINEISKIISAAGNFDVKEERAAASLLQLSGNASDFSYGRNSITLTASA"
     gene            9224..11347
                     /locus_tag="ROI21_004001"
     CDS             9224..11347
                     /locus_tag="ROI21_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDX5112866.1"
                     /translation="MDSCHKIDYGLYALEILAQYHNVSVNPEEIKHRFDTDGTGLGLT
                     SWLLAAKSLELKVKQVKKTIDRLNFISLPALVWREDGRHFILTKVSKEANRYLIFDLE
                     QRNPRVLEQSEFEALYQGHIILIASRSSVTGKLAKFDFTWFIPAIIKYRKIFIETLVV
                     SVFLQLFALITPLFFQVVMDKVLVHRGFSTLNVITVALSVVVVFEIILSGLRTYIFAH
                     STSRIDVELGAKLFRHLLALPISYFESRRVGDTVARVRELDQIRNFLTGQALTSVLDL
                     LFSFIFFAVMWYYSPKLTLVILFSLPCYAAWSVFISPILRRRLDDKFSRNADNQSFLV
                     ESVTAINTIKAMAVSPQMTNIWDKQLAGYVAAGFKVTVLATIGQQGIQLIQKTVMIIN
                     LWLGAHLVISGDLSIGQLIAFNMLAGQIVAPVIRLAQIWQDFQQVGISVTRLGDVLNS
                     PTESYHGKLALPEINGNITFRNIRFRYKPDSPVILDNINLSIKQGEVIGIVGRSGSGK
                     STLTKLIQRFYIPENGQVLIDGHDLALADPNWLRRQVGVVLQDNVLLNRSIIDNISLA
                     NPGMSVEKVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNNP
                     KILIFDEATSALDYESEHIIMRNMHKICKGRTVIIIAHRLSTVKNADRIIVMEKGKIV
                     EQGKHKELLSEPESLYSYLYQLQSD"
     gene            11366..12802
                     /gene="hlyD"
                     /locus_tag="ROI21_004002"
     CDS             11366..12802
                     /gene="hlyD"
                     /locus_tag="ROI21_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin T1SS ABC transporter subunit
                     HlyD"
                     /protein_id="HDX5112867.1"
                     /translation="MKTWLMGFSEFLLRYKLVWSETWKIRKQLDTPVREKDENEFLPA
                     HLELIETPVSRRPRLVAYFIMGFLVIAVILSVLGQVEIVATANGKLTLSGRSKEIKPI
                     ENSIVKEIIVKEGESVRKGDVLLKLTALGAEADTLKTQSSLLQTRLEQTRYQILSRSI
                     ELNKLPELKLPDEPYFQNVSEEEVLRLTSLIKEQFSTWQNQKYQKELNLDKKRAERLT
                     ILARINRYENLSRVEKSRLDDFRSLLHKQAIAKHAVLEQENKYVEAANELRVYKSQLE
                     QIESEILSAKEEYQLVTQLFKNEILDKLRQTTDNIELLTLELEKNEERQQASVIRAPV
                     SGKVQQLKVHTEGGVVTTAETLMVIVPEDDTLEVTALVQNKDIGFINVGQNAIIKVEA
                     FPYTRYGYLVGKVKNINLDAIEDQKLGLVFNVIVSVEENDLSTGNKHIPLSSGMAVTA
                     EIKTGMRSVISYLLSPLEESVTESLHER"
BASE COUNT         3967 a         2466 c         2922 g         3793 t
ORIGIN      
        1 gtcaatacgg tttttgctgc acgcttaccc atggaacgta gtactcacca ataaataagc
       61 gtcaatacgg tgctccgttg acgcttacag tcgcagaggg aattgcttgg ataattcgcc
      121 gatggaaaga gtctttcgca gcctgaaaag tgaatggctt ccgaaaggtg gttatggtga
      181 ttttagccat gcggtacgcg atataaacca gtggattaat ggttgttata ttataacgtt
      241 tatcgacctc acacgaacaa cgatggttta ccgccttgct tacatgaaga aaagtgggaa
      301 caggttatcc cggtgtcctg attttgtgct ccactacaga acttgtatcc cttttacaaa
      361 ggcagataca cataaccacc ccaaaatatg ccgcctgatg ttataatgga agaaaaaaac
      421 gaaagcaggc tccagtcgtt gaatcagtca acctgatatc accaccgtgt agtaccagca
      481 tcctgcgtac aatcagcaat cccagtcctc cccggggctc ttctgacggt tcatgacata
      541 acactgatgc ccgccggaaa agatgagttc gcatatcttc agttagtcca gggccgctgt
      601 cagccacttc gacgtgcaac cgattatctt cctgccagac tttcagcctg atcgagcctt
      661 ccggaggtgt gtggcgtaca gcattatcca gtaaatttgt tatcacacgc tctatcattg
      721 aaatatctgc cctgataaga ggcagggaat gtgacatcat aattcttagt tgcaatcgac
      781 gggtttctat gctgagctca aatttttgag ctacatcctg aataagttca cagacagaaa
      841 attgctccag ttgaggtttt atagcaccat gctcaagacg tgccagctca aaaagctgac
      901 aggatagatg cctcaccttg tgtccctgcc ggagagctgt tgtcagatat ttatgacagt
      961 cctctgatga tagcgaatcc gacttcattg acagggtttc cagatatccc agaagtgatg
     1021 ttaatggcgt ccgtaaatca tgggagatat tggcaataaa ttcacggcgc tgttgatcac
     1081 tttctgaaag ttgatcccac tgacaggata ttttacgggc cagttcaatg aaggcattgt
     1141 gtaatatctc gacctcgttg ctaggggggg tggcagggcg ctgaattgcc agttgtttaa
     1201 tggcactaat actgtcctgc tctatcccgc tgacattttc agttagttgc tgtatgggac
     1261 gggttaccca gtaccagacc agcatacccg acagcagacc acatatcact accagaaaca
     1321 gcgaccagta cagtgcattc catagtgcct ttgtccaggc tgcatcagta agagcattaa
     1381 gttcctctcc ctgtaaaata atatacagat atcctttcag atcatccctc aggtaaagcg
     1441 gtgcggtact gaaaactttt ttcttattta cacttcgggg atcatcacca tatacgggcc
     1501 agacagcacc ggagagaaat tttttcaagg gcgctatatt gatataccga cgtttgatat
     1561 gacctagagg ggcggcctcc acaagcagcc gaccttccgg cgagacaata tacagctcca
     1621 cactgggatt aagcgtcatc agacgctcaa acagactctt taatgtccgg tgattcacct
     1681 gcccacgatt atccagcaga ggctccgtga tgacaatctg ttgcgccagc cctgcagata
     1741 aacgctgtac cattgcattg ccatacagat tactgctgta aatatgaacg ccacatgtca
     1801 ctgctgcgca aaacagcaaa acgactgtaa atactaggct taacctctgg cttaatgtca
     1861 ggctacaatt catttatcgc attcctgcat tgtcaactga aaacctatac ccttttcccc
     1921 agacggtctg gatcatcttt ggctctgctg catcctgttc aattttggca cgaagacggt
     1981 tgatatgcgt gttgactgta tgctcatatc cttcatgctg atacccccag acattatcca
     2041 gcagtgaaag acgggaaaaa acttcgccag gatgacgtgc aaaccagagc agcagatcaa
     2101 actcgcgtgg ggtaagatca acctgtttat tatgcaaatg aacttcacgt gaaaatggat
     2161 tgatgcacag atcgtgacag caaatcagtc cacctgccag gagaatattt tgccccatgg
     2221 cttcctgacg acgaaacaac gctttgatgc gggcaatcag ctccggaatg gaaaagggtt
     2281 tcggtagata gtcatcagcc cccatttcca gtcccaggac gcggtgggtt tcgctggtac
     2341 gggcactgat gataatcaca ggcagataac gggtcatctg acggatataa cggcaaatct
     2401 ccagcccatt aacattaggc agcataagat caagtattac ggcatcccag gtctgcttgt
     2461 ctagtaataa acgagctctg gcgccatccg cttcatgaac tatctgatac ccttcatcct
     2521 gcagattaag ccgcaacaga gctgcaatat cataatcatc ttccatcagc agtatttttt
     2581 ttacgttatt catcaaaagt tatccacata ttctttatct gaattttacc ccagcatttg
     2641 acgatgaatt atcacatttt ttttaaactc ttgtgaggat gtgatgaaca ttcctttcca
     2701 taatatactt agtttacaga cagagatcac ataatgcagc cattaccgtt aaaacaaata
     2761 catcctctgt ggttacgctg ttgccaccgg attaatgctg ttactgtcgc aggcatgata
     2821 tgtagtggat ggcgcattta taatgcttct cctttgtttt cttttaacta tccggcagca
     2881 ttaactctcg gtggctggct tggtggtgcg ttactgtggc acttcgccct gatgtggatt
     2941 ctgctgatta atgccacgat ctatcttact ctgggaattt gtagcggacg tttccggagc
     3001 aagtttttcc cgctgtcatg gacctcactg aaagaatccc ttacgcaggc actgtatgga
     3061 aagctgcatc atacgaatat gcaggaatac aatatggtgc aaaaagtcgc ctatattctg
     3121 ttattttaga tggcgctgct ctgctattgt ctggtctggt tttatggaaa cccgttcagt
     3181 tttcccttct gactatacta ttcggtggtt atgatacagt gcgttatatc cactttctgt
     3241 gcatgaacat tatggtactg tttattatta ttcacctggt tatggtgtta ttggttccgc
     3301 gtgcattgtt atctatgata cggggtcact aatgaagaaa aaacattttt tgtctgacag
     3361 tgatgcccat tacgtggtga aagatgcaga aaaaatcctt gcgccggaca gacgtcgttt
     3421 actccaacag ggactgacgc ttgggggaat tatgatgctg actggctgtg atataagcaa
     3481 taatgatgat attgaaaaag cattgttccg tatatccaga tttaatgacc ggattcaaag
     3541 ttttctgttc agcaaaaaca gtgtggcacc agaatatctt ccagaggaca ttaccactcc
     3601 attccctttt aatgccttct atggagaaga agagatccca gtaattgatg gccaggatta
     3661 taaattggaa atacgggggc tggtacgtga taaccgtaac tggtttttgt ctgaattgta
     3721 taacatgcag catgtgagtc agataacccg tcatatctgt gttgaaggat ggagtgcaat
     3781 aggaaaatgg ggagggatac cattccggca tttcctgacg ctgattggtg ccgatatgcg
     3841 ggcaaaatac gtcagttttc gttgcgtcga tggttactac accagtattg atatggcgac
     3901 agcactacat tcacaaacat tgctgacatt gacatggaat aacagggtat tgccaaaaga
     3961 atatggctat cccataaaga tacgtatccc tactaagctg ggatataaaa accccaagca
     4021 tgttcaggtt atagaggtca ctaatcattt tccggggggg tactgggaag actagggtta
     4081 taactggttt ggtggttgtt gatacaattg ttaaaaatga ggaggaacca tgaaaaatat
     4141 tatttctgta actgtactat ccgcgctgtt catgttcaat gctcaggctg cagacacaat
     4201 ggataaaaca catgaagtaa aaggcagcat gtccagcgcg catgataaaa tgtgcaagga
     4261 tggtatgtgc aaaaatgcaa tggataaaga gaaaatggag aaagaacaca tgagcaaaac
     4321 ggatgggatg aaaaatgacc acatgggtaa aaaagacaat atgtctcaat aagttgagaa
     4381 cttaaaatta cgttacgata aaacatcata ttctgtcgtt tttcagataa atagctcatt
     4441 atggcaatgt caggctctgt atcggaaatg acattgcctt tactgtgtat tcgtaagcat
     4501 catttctgcg cagtctggca gatcctgaaa tccttatgaa acaaagtata gtgagtgaaa
     4561 tcataaaacc gactgactgg ttaactggtc tgatttgttt cagtcagttt tcgttaaaat
     4621 tctccatgtt ataaatttaa taacctgaaa attattaccg cgctaattaa atgtatttct
     4681 ttttgttatt ttttttctgg agttttatac tgatttaagt aatatatttc aaataaactc
     4741 gtttttacta tgtcttatgg tgtaatgcgc taacctgttt cgatacggta atagatatac
     4801 ccaccatatt gaacatttgt agatcattat tctatagtgc tcagagttaa aatcgtctct
     4861 tgttgaattg atttgttaca caggattctg gtcccggttg atacgggtaa tttccggaag
     4921 ggcggtagca tgcttattca aaatacaata tgagacacgg tcgaagtcgg atggaattaa
     4981 atcatggcta cagcattttt gtgtgtatga tataattttg cctcatattt tagagtatac
     5041 ttgcgcaccc gccaatagag tcgccttctt ttgcgtgctg ggatcgtact gtatgagata
     5101 cactttgaat cattatccga agtcagaaga agcgcattga ctggtatgac ctgattaatt
     5161 caacccctta tctgacaggg gtagaaaaag aagagaggtt cacatgacaa ctcacaggtc
     5221 atcattcggc agctgattgg tactgggtaa ggaagttata tatataagca actatacatc
     5281 ttagatgtat gataaagaaa aagataacag ttctttagaa tgtgcatatt gaagagaata
     5341 caatagcatg gtttatataa attacgcata aaaataagca tatgtaagca ttttggtttg
     5401 ctttttttta cctgccaccg caatgaatgc tttttttatg ttaatgtgcg ttatgaaact
     5461 aaatgcaaga aacatattta aaggattaat atcgttctct cacagactcc gtttacttat
     5521 tcaagaatat aatttaattt atagtgagct tattatgaat atgaacaatc cattagaggt
     5581 tcttgggcat gtatcctggc tctgggccag ttccccatta cacagaaact ggccagtctc
     5641 tttgtttgca ataaatgtat tacctgcaat acgggctaac caatatgctt tattaacccg
     5701 ggataattac cctgttgcat attgtagttg ggctaattta agtttagaaa atgaaattaa
     5761 atatcttaat gatgttacct cattagtcgc agaagactgg acttcaggtg atcgtaaatg
     5821 gttcattgac tggattgctc ctttcgggga taacggtgcc ctgtacaaat atatgcgaaa
     5881 aaaattccct gatgaactat tccgagccat cagggtggat cccaaaactc atgttggtaa
     5941 agtatcagaa tttcacggag gtaaaattga taaacagtta gcgaataaaa tttttaaaca
     6001 atattaccac gagttaataa ctgaagtaaa aaacaagaca gatttcaatt tttcattaac
     6061 aggttaagag ataattaaat gccaacaata accactgcac aaattaaaag cacactacag
     6121 tctgcaaagc aatcctctgc aaataaattg cactcagcag gacaaagcac gaaagatgca
     6181 ttaaaaaaag cagcagagca aacccgcaat gcgggaaaca gactcatttt acttatccct
     6241 aaagattata aaggacaggg ttcaagcctt aatgaccttg tcaggacggc agatgaactg
     6301 ggaattgaag tccagtatga tgaaaagaat ggcacggcga ttactaaaca ggtattcggc
     6361 acagcagaga aactcattgg cctcaccgaa cggggagtga ctatctttgc accacaatta
     6421 gacaaattac tgcaaaagta tcaaaaagcg ggtaataaat taggcggcag tgctgaaaat
     6481 ataggtgata acttaggaaa ggcaggcagt gtactgtcaa cgtttcaaaa ttttctgggt
     6541 actgcacttt cctcaatgaa aatagacgaa ctgataaaga gacaaaaatc tggtagcaat
     6601 gtcagttctt ctgaactggc aaaagcgagt attgagctaa tcaaccaact cgtggacaca
     6661 gctgccagca ttaataataa tgttaactca ttttctcaac aactcaataa gctgggaagt
     6721 gtattatcca atacaaagca cctgaacggt gttggtaata agttacagaa tttacctaac
     6781 cttgataata tcggtgcagg gttagatact gtatcgggta ttttatctgc gatttcagca
     6841 agcttcattc tgagcaatgc agatgcagat accggaacta aagctgcagc aggtgttgaa
     6901 ttaacaacga aagtactggg taatgttgga aaaggtattt ctcaatatat tatcgcacag
     6961 cgcgctgcac agggattatc tacatctgct gctgctgccg gtttaattgc ttctgtagtg
     7021 acattagcaa ttagtcccct ctcattcctg tccattgccg ataagtttaa acgtgccaat
     7081 aaaatagagg agtattcaca acgattcaaa aaacttggat acgatggtga cagtttactt
     7141 gctgcttttc acaaagaaac aggagctatt gatgcatcgt taacaacgat aagcactgtt
     7201 ctggcttcag tatcttcagg tattagtgct gctgcaacga catctctggt tggtgcaccg
     7261 gtaagcgcgc tggtaggggc tgttacgggg ataatttcag gcatccttga ggcttcaaaa
     7321 caggcaatgt ttgaacatgt cgccagtaaa atggccgatg ttattgctga atgggagaaa
     7381 aaacacggca aaaattactt tgaaaatgga tatgatgccc gccatgctgc atttttagaa
     7441 gataacttta aaatattatc tcagtataat aaagagtatt ctgttgaaag atcagtcctc
     7501 attacccagc aacattggga tacgctgata ggtgagttag cgggtgtcac cagaaatgga
     7561 gacaaaacac tcagtggtaa aagttatatt gactattatg aagaaggaaa acgtctggag
     7621 aaaaaaccgg atgaattcca gaagcaagtc tttgacccat tgaaaggaaa tattgacctt
     7681 tctgacagca aatcttctac gttattgaaa tttgttacgc cattgttaac tcccggtgag
     7741 gaaattcgtg aaaggaggca gtccggaaaa tatgaatata ttaccgagtt attagtcaag
     7801 ggtgttgata aatggacggt gaagggggtt caggacaagg ggtctgtata tgattactct
     7861 aacctgattc agcatgcatc agtcggtaat aaccagtatc gggaaattcg tattgagtca
     7921 cacctgggag acggggatga taaggtcttt ttatctgccg gctcagccaa tatctacgca
     7981 ggtaaaggac atgatgttgt ttattatgat aaaacagaca ccggttatct gaccattgat
     8041 ggcacaaaag caaccgaagc gggtaattac acggtaacac gtgtacttgg tggtgatgtt
     8101 aagattttac aggaagttgt gaaggagcag gaggtttcag ttggaaaaag aactgaaaaa
     8161 acgcaatatc ggagttatga attcactcat atcaatggta aaaatttaac agagactgat
     8221 aacttatatt ccgtggaaga acttattggg accacgcgtg ccgacaagtt ttttggcagt
     8281 aaatttactg atatcttcca tggcgcggat ggtgatgacc atatagaagg aaatgatggg
     8341 aatgaccgct tatatggtga taaaggtaat gatacgctga ggggcggaaa cggggatgac
     8401 cagctctatg gcggtgatgg caatgataag ttaattgggg ggacaggtaa taattacctt
     8461 aacggcggtg acggagatga tgagcttcag gttcagggga attctcttgc taaaaatgta
     8521 ttatccggtg gaaaaggtaa tgacaagttg tacggcagtg agggagcaga tctgcttgat
     8581 ggcggagaag ggaatgatct tctgaaaggt ggatatggta atgatattta tcgttatctt
     8641 tcaggatatg gccatcatat tattgacgat gatgggggga aagacgataa actcagtttg
     8701 gctgatattg atttccggga tgtggccttc aggcgagaag ataatgacct catcatgtat
     8761 aaagctgaag gtaatgttct ttccattggt cataaaaatg gtattacatt caggaactgg
     8821 tttgaaaaag agtcaggtga tatctctaat caccagatag agcagatttt tgataaagac
     8881 ggcagggtaa tcacaccaga ttcccttaaa aaggcacttg agtatcaaca gagtaataat
     8941 aaggcaagtt atgtgtatgg gaatgatgca ttagcctatg gaagtcagga taatcttaat
     9001 ccattaatta atgaaatcag caaaatcatt tcagctgcag gtaattttga tgttaaagag
     9061 gaaagagctg cagcttcttt attgcagttg tccggtaatg ccagtgattt ttcatatgga
     9121 cggaactcaa taactttgac agcatcagca taatttatta atttaaataa tagcaatctt
     9181 actgggctgt gccacataag attgctattt ttttggagtc ataatggatt cttgtcataa
     9241 aattgattat gggttatacg ccctggagat tttagcccaa taccataacg tctctgttaa
     9301 cccggaagaa attaaacata gatttgacac agacgggact ggtctgggat taacgtcatg
     9361 gttgcttgct gcgaaatctt tagaactaaa ggtaaaacag gtaaaaaaaa caattgaccg
     9421 attaaacttt atttctttgc ccgcattagt ctggagagag gatggacgtc attttattct
     9481 gactaaagtc agtaaagaag caaacagata tcttattttt gatctggagc aacgaaatcc
     9541 ccgtgttctc gaacagtctg agtttgaggc gttatatcag gggcatatta ttcttattgc
     9601 ttcccgttct tctgttaccg ggaaactggc aaaatttgac tttacctggt ttatccctgc
     9661 cattataaaa tacagaaaaa tatttattga aacccttgtt gtatctgttt ttttacaatt
     9721 atttgcatta ataacccccc ttttttttca ggtggttatg gacaaagtat tagtacacag
     9781 ggggttttca acccttaatg ttattactgt cgcattatct gttgtggtgg tgtttgagat
     9841 tatactcagc ggtttaagaa cttacatttt tgcacatagt acaagtcgga ttgatgttga
     9901 gttgggtgcc aaactcttcc ggcatttact ggcgctaccg atctcttatt ttgagagtcg
     9961 tcgtgttggt gatactgttg ccagggtaag agaattagac cagatccgta attttctgac
    10021 aggacaggca ttaacatctg ttctggactt attattttca ttcatatttt ttgcggtaat
    10081 gtggtattac agcccaaagc ttactctggt gatcttattt tcgctgccct gttatgctgc
    10141 atggtctgtt tttattagcc ccattttgcg acgtcgcctt gatgataagt tttcacggaa
    10201 tgcggataat caatctttcc tggtggaatc agtcacggcg attaacacta taaaagctat
    10261 ggcagtctca cctcagatga cgaacatatg ggacaaacaa ttggcaggat atgttgctgc
    10321 aggctttaaa gtgacagtat tagccaccat tggtcaacaa ggaatacagt taatacaaaa
    10381 gactgttatg atcatcaacc tgtggttggg agcacacctg gttatttccg gggatttaag
    10441 tattggtcag ttaattgctt ttaatatgct tgctggtcag attgttgcac cggttattcg
    10501 ccttgcacaa atctggcagg atttccagca ggttggtata tcagttaccc gccttggtga
    10561 tgtgcttaac tctccaactg aaagttatca tgggaaactg gcattaccgg aaattaatgg
    10621 taatatcact tttcgtaata tccggtttcg ctataagcct gactctccgg ttattttaga
    10681 taatatcaat ctcagtatta agcaggggga ggttattggt attgtcggac gttctggttc
    10741 aggaaaaagc acattaacta aattaattca acgtttttat attcctgaaa atggccaggt
    10801 cttaattgat ggacatgatc ttgcgttggc cgatcctaac tggttacgtc gtcaggtggg
    10861 ggttgtgttg caggacaatg tgctgcttaa tcgcagtatt attgataata tctcactggc
    10921 taatcctggt atgtccgtcg aaaaagttat ttatgcagcg aaattagcag gcgctcatga
    10981 ttttatttct gaattgcgtg aggggtataa caccattgtc ggggaacagg gggcaggatt
    11041 atccggaggt caacgtcaac gcatcgcaat tgcaagggcg ctggtgaaca accctaaaat
    11101 acttattttt gatgaagcaa ccagtgctct ggattatgag tcggagcata tcatcatgcg
    11161 caatatgcac aaaatatgta agggcagaac ggttataatc attgctcatc gtctgtctac
    11221 agtaaaaaat gcagaccgca ttattgtcat ggaaaaaggg aaaattgttg aacagggtaa
    11281 acataaggaa ctgctttctg aaccggaaag tttatacagt tacttatatc agttacagtc
    11341 agactaacag aaagaacaga agaatatgaa aacatggtta atggggttca gcgagttcct
    11401 gttgcgctat aaacttgtct ggagtgaaac atggaaaatc cggaagcaat tagatactcc
    11461 ggtacgtgaa aaggacgaaa atgaattctt acccgctcat ctggaattaa ttgaaacgcc
    11521 ggtatccaga cggccgcgtc tggttgctta ttttattatg gggtttctgg ttattgctgt
    11581 cattttatct gttttaggtc aggtggaaat tgttgccact gcaaatggga aattaacact
    11641 aagtgggcgc agcaaagaaa ttaaacctat tgaaaactca atagttaaag aaattatcgt
    11701 aaaagaagga gagtcagtcc ggaaagggga tgtgttatta aagcttacag cactgggagc
    11761 tgaagctgat acgttaaaaa cacagtcatc actgttacag accaggctgg aacaaactcg
    11821 gtatcaaatt ctgagcaggt caattgaatt aaataaacta cctgaactga agcttcctga
    11881 tgagccttat tttcagaatg tatctgaaga ggaagtactg cgtttaactt ctttgataaa
    11941 agaacagttt tccacatggc aaaatcagaa gtatcaaaaa gaactgaatc tggataagaa
    12001 aagagcagag cgattaacaa tacttgcccg tataaaccgt tatgaaaatt tatcgagagt
    12061 tgaaaaaagc cgtctggatg atttcaggag tttattgcat aaacaggcaa ttgcaaaaca
    12121 tgctgtactt gagcaggaga ataaatatgt cgaggcagca aatgaattac gggtttataa
    12181 atcgcaactg gagcaaattg agagtgagat attgtctgca aaagaagaat atcagcttgt
    12241 cacgcagctt tttaaaaatg aaattttaga caagctaaga caaacaacag acaacattga
    12301 gttattaact ctggagttag agaaaaatga agagcgtcaa caggcttcag taatcagggc
    12361 ccctgtttcg ggaaaagttc agcaactgaa ggttcatact gaaggtgggg ttgttacaac
    12421 agcggaaaca ctgatggtca tcgttccgga agatgacacg ctggaggtta ctgctctggt
    12481 acaaaataaa gatattggtt ttattaacgt cgggcagaat gccatcatta aagtggaggc
    12541 ctttccttac acccgatatg gttatctggt gggtaaggtg aaaaatataa atttagatgc
    12601 aatagaagac cagaaactgg gactcgtttt taatgtcatt gtttctgttg aagagaatga
    12661 tttgtcaacc gggaataagc acattccatt aagctcgggt atggctgtca ctgcagaaat
    12721 aaagactgga atgcgaagcg taatcagcta tcttcttagt cctctggaag agtctgtaac
    12781 agaaagttta catgagcgtt aagtctcaga gccgcggtat ccggctcata tcttctcctg
    12841 tcatgtgtga ctgcactgtt ttgtggcaaa ccagtactga ctatccactg gaattcggta
    12901 gtcattaact atggcattga tgtcctttat aactgtctta cagggcgtat aacacatccc
    12961 gcgacggaag cttatcacag aggaagaggt aagcttcaga atggaaagta tatgagaaga
    13021 ctgtgattgt ttaaaaatac acaatccgct gtgaggtgga gtcaggggag tgaggtgtac
    13081 tggcaatagc ggatactacc atttgttctt tttttaagca gccatctgat gatatttttc
    13141 cctgaagg
//
LOCUS       DAPOZO010000117        13114 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPOZO010000117 DAPOZO010000000
VERSION     DAPOZO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.117"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            214..555
                     /gene="raiA"
                     /locus_tag="ROI21_004003"
     CDS             214..555
                     /gene="raiA"
                     /locus_tag="ROI21_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDX5112868.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    658..783
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            659..706
                     /gene="pheL"
                     /locus_tag="ROI21_004004"
     CDS             659..706
                     /gene="pheL"
                     /locus_tag="ROI21_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDX5112869.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            805..1965
                     /gene="pheA"
                     /locus_tag="ROI21_004005"
     CDS             805..1965
                     /gene="pheA"
                     /locus_tag="ROI21_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDX5112870.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     QVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPY
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGDITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2008..3129)
                     /gene="tyrA"
                     /locus_tag="ROI21_004006"
     CDS             complement(2008..3129)
                     /gene="tyrA"
                     /locus_tag="ROI21_004006"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDX5112871.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRH"
     gene            complement(3140..4210)
                     /gene="aroF"
                     /locus_tag="ROI21_004007"
     CDS             complement(3140..4210)
                     /gene="aroF"
                     /locus_tag="ROI21_004007"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDX5112872.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4420..4785
                     /locus_tag="ROI21_004008"
     CDS             4420..4785
                     /locus_tag="ROI21_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDX5112873.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     STIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4935..5453
                     /locus_tag="ROI21_004009"
     CDS             4935..5453
                     /locus_tag="ROI21_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDX5112874.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSRALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5443..6669
                     /gene="dgcN"
                     /locus_tag="ROI21_004010"
     CDS             5443..6669
                     /gene="dgcN"
                     /locus_tag="ROI21_004010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDX5112875.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGSAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIVQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTACILLASGIAITLTRHLHKGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNSSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLQHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNRPFDLHNGHQTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            6685..7167
                     /locus_tag="ROI21_004011"
     CDS             6685..7167
                     /locus_tag="ROI21_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDX5112876.1"
                     /translation="MIKHLVAPLIFTSLVLTGCQSPQGKFTPEQVVAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGKIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7244..7591)
                     /gene="rplS"
                     /locus_tag="ROI21_004012"
     CDS             complement(7244..7591)
                     /gene="rplS"
                     /locus_tag="ROI21_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDX5112877.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7633..8400)
                     /gene="trmD"
                     /locus_tag="ROI21_004013"
     CDS             complement(7633..8400)
                     /gene="trmD"
                     /locus_tag="ROI21_004013"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDX5112878.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPSVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8431..8979)
                     /gene="rimM"
                     /locus_tag="ROI21_004014"
     CDS             complement(8431..8979)
                     /gene="rimM"
                     /locus_tag="ROI21_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDX5112879.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8998..9246)
                     /gene="rpsP"
                     /locus_tag="ROI21_004015"
     CDS             complement(8998..9246)
                     /gene="rpsP"
                     /locus_tag="ROI21_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDX5112880.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9495..10856)
                     /gene="ffh"
                     /locus_tag="ROI21_004016"
     CDS             complement(9495..10856)
                     /gene="ffh"
                     /locus_tag="ROI21_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDX5112881.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            11023..11814
                     /locus_tag="ROI21_004017"
     CDS             11023..11814
                     /locus_tag="ROI21_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDX5112882.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11835..>13114
                     /locus_tag="ROI21_004018"
     CDS             11835..>13114
                     /locus_tag="ROI21_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDX5112883.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVA"
BASE COUNT         3317 a         3442 c         3083 g         3272 t
ORIGIN      
        1 ttgtgaaact gaagcgattt agtcactatc gatctcatca aatatggctc gctttgagat
       61 attcctcaag taaaaaaaca cctcttcctg cgatttctca caaaaaaact tcgttgacaa
      121 aaagtgacaa aattatgaga tttccatcac acattttgac atcaggaacg gtatgctgaa
      181 ttcaccaaga cgggaagaca agaggtaaaa tttatgacaa tgaacattac cagcaaacaa
      241 atggaaatta ctccggccat ccgccaacat gtcgcagacc gtctcgccaa actggaaaaa
      301 tggcaaacac atctgattaa tccacatatc attctgtcca aagagccaca agggtttgtt
      361 gctgacgcca caatcaatac acctaacggc gttctggttg ccagtggtaa acatgaagat
      421 atgtacaccg caattaacga attgatcaac aagctggaac ggcagctcaa taaactgcag
      481 cacaaaggcg aagcacgtcg tgccgcaaca tcggtgaaag acgccaactt cgtcgaagaa
      541 gttgaagaag agtagtcctt tatattgagt gtatcgccaa cgcgccttcg ggcgcgtttt
      601 ttgttgacag cgtgaaaaca gtacgggtac tgtactgaag tcacttaagg aaacaaacat
      661 gaaacacaca ccgtttttct tcgcattctt ttttaccttc ccctgaatgg gaggcgtttc
      721 gtcgtgtgaa acagaatgcg aagacgaaca ataaggcctc ccaaatcggg gggtcttttt
      781 tattgataac aaaaaggcaa cactatgaca tcggaaaatc cattactggc gctgcgagag
      841 aaaatcagcg cgctggatga aaaattatta gcattactgg cagagcggcg cgaactggcc
      901 gtcgaggtgg gaaaagccaa actgctctcg catcgccagg tacgtgatat tgatcgtgaa
      961 cgcgatttac tggaaagatt aattacgctc ggtaaagcgc accatctgga cgcccattac
     1021 attactcgcc tgttccagct catcattgaa gattccgtat taactcagca ggctttgctc
     1081 cagcaacatc tcaataaaat taatccgtac tcagcacgca tcgcttttct cggccccaaa
     1141 ggttcttatt cccatcttgc ggcgcgccag tatgctgccc gtcactttga gcaattcatt
     1201 gaaagtggct gcgccaaatt tgccgatatt tttaatcagg tggaaaccgg ccaggccgac
     1261 tatgccgtcg taccgattga aaataccagc tccggtgcca taaatgacgt ttacgatctg
     1321 ctgcaacata ccagtttgtc gattgttggc gagatgacgt taactatcga ccattgtttg
     1381 ttggtctccg gcactactga tttatccacc atcaatacgg tctacagcca tccgcagcca
     1441 ttccagcaat gcagcaaatt ccttaatcgt tatccgcact ggaagattga atataccgaa
     1501 agtacgtctg cggcaatgga aaaggttgca caggcaaaat caccgcatgt tgctgcgttg
     1561 ggaagcgaag ctggcggcac tttgtacggt ttgcaggtac tggagcgtat tgaagcgaat
     1621 cagcgacaaa acttcacccg atttgtggtg ttggcacgta aagccattaa cgtgtctgac
     1681 caggttccag cgaaaacgac gttgttaatg gcgaccgggc aacaagctgg tgcgctggtt
     1741 gaagcgttgc tggtactgcg caaccacaat ctgattatga cccgtctgga gtcacgcccg
     1801 attcacggta atccatggga agagatgttt tatctggata ttcaggccaa tcttgaatca
     1861 gcggaaatgc aaaaagcatt gaaagagtta ggggacatca cccgttcgat gaaggtattg
     1921 ggctgttacc caagtgagaa cgtagtgcct gttgatccaa cctgatgaaa aggtgccgga
     1981 tgatgtgaat cacccggcac tggattatta gtggcggttg tcattcgcct gacgcaataa
     2041 cacgcggctt tcactctgaa aacgctgtgc gtaatcgccg aaccagtgct ccaccttgcg
     2101 gaaactgtca ataaacgcct gcttatcgcc ctgctccagc aactcaatcg cctcgccgaa
     2161 acgcttatag taacgtttga ttaacgccag attacgctct gacgacataa taatgtcggc
     2221 ataaagctgc ggatcctgag caaacagtcg cccaaccatc gccagttcaa ggcggtaaat
     2281 cggcgaagag agcgccagaa gttgctcaag ctgaacattt tcttctgcca ggtgcagccc
     2341 ataagcaaaa gtagcaaagt ggcgcagagc ctgaataaac gccatattct gatcgtgctc
     2401 gacagcgcta atacgatgca gccgagcgcc ccagacctga atttgctcca gaaaccattg
     2461 gtatgcttcc ggcttacgtc catcacacca gaccacaact tgctttgcca ggctaccgct
     2521 gtccggaccg aacatcgggt gtagccccag caccggacca tcatgcgccg ccagcatggc
     2581 ctgtaatggc ccatttttca ctgatgccag atcgaccaga atacaatctt tcggtaaagg
     2641 cggtaatttg ccgataactt gctcagtaac gtggattggc acactaacaa tcaccattcc
     2701 ggcatcggca acaatatcag ccgctcgatc ccagtcatgt tgctccagaa tccgcacctg
     2761 ataacccgat agtgtcagca tcttctcgaa caggcgtccc atctgaccgc caccgccgac
     2821 gataaccacc ggacgcagtg acggacaaag tgttttaaat cctttgtcat tttcactgga
     2881 gtaagattca cgcatcaccc gacgcaaaac atcctcaatc agatctggcg gtacacccag
     2941 agcttccgcc tctgcacgac gcgaggccaa catagatgcc tcgcgctccg gcacataaat
     3001 aggcagtcca aagcggcttt tcacctcgcc cacttcagca accagttcca gacgcttcgc
     3061 taataaattc agcagcgctt tatcgacttc atcaatttga tcgcgtaatg cggtcaactc
     3121 agcaaccata ataaacctct taagccacgc gagccgtcag ctgcccgttc agatcctgat
     3181 gaatttcacg cagcaaggca tcggtcattt cccaactaat gcaggcatcg gttacggata
     3241 caccgtattt catttcactg cgcggttgct cggaagactg attgccctcg tggatattac
     3301 tttcgatcat cagaccaata attgagcgat tgccatcttt gatttgagca accacggatt
     3361 ctgccaccgc aggctgacgg cgataatctt tattggaatt accgtggctg caatctacca
     3421 tcagagacgg gcgcagtccc gcctgttcca tctctttttc acattgcgca acatccgcag
     3481 ggctatagtt cggcgcttta ccaccgcgca ggatcacatg cccgtccgga ttcccctgag
     3541 tttgtagcaa cgccacctgc cctgcctggt taatgccaac aaaacggtgc ggctgggcgg
     3601 cggcgcgcat agcgttaatt gctgttgcca gactgccgtc ggtgccgttt ttaaaaccaa
     3661 ccggcatgga aagcccggag gccatttcac ggtgagtttg cgattccgtt gtacgagcac
     3721 caattgctga ccagctaaac agatcgccca ggtattgcgg gctatttgga tctaacgctt
     3781 ccgtcgccag tggcagtccc atattcacca gctcaagcag caatttacgc gcgatctgca
     3841 gcccggcttc tacatcaaaa gagccatcca tatggggatc gttaatcaac cctttccaac
     3901 cgacagtggt acggggtttt tcaaaataga cgcgcattac cagatagagg ctatcgctga
     3961 cctctgcggc aagggcttta aatcgacgag catattccag agcagtttcc ggatcatgaa
     4021 tggaacaagg accacatact accaacagac gaggatcgcg cccggcgata atatctgaaa
     4081 tggttttacg cgagtcagca atctgggctt cttgttgcag gcttaatgga aaagcggcct
     4141 tcagttgttc cggagtcatt aaaacctgtt cgtcggtaat atgtacgtta ttcagcgcgt
     4201 ctttttgcat gatggcgatc ctgtttatgc tcgtttgcga tagttgatcc tcagcgagga
     4261 tgacgtaacg ataacacata aagtaaagtt ttcaatccat atttcgtaca tttttattta
     4321 cacaagcaat ttattcgcgc ttttgacgct tattttcgta caaataaatt tacacctcct
     4381 ttaaaaacaa tccgctatgc tttgaaaaag gagaaagaaa tgatgaaaaa gtttattgcc
     4441 cccttgttgg ctttactggt tagcggatgt cagattgatc cttatactca cgcgccaacc
     4501 ttgaccagca ccgactggta tgatgtcggt atggaagatg cgatatcggg cagcaccata
     4561 aaagatgacg atgcatttag cgattcacag gcggatcgcg gtctatacct taaaggatat
     4621 gccgaaggac aaaagaaaac ttgtcagacc gattttactt atgcccgagg actttccggt
     4681 aaaagctttc ctgccagctg caacaatgtt gaaaatgcca gccaactgca tgaagtatgg
     4741 caaaaagggg ctgatgaaaa tgccagcaca atacgattaa attaattaaa cccccgcaaa
     4801 taaattaagc atgagatttt gctcattgtg ttttatttct cacattgatg acgaccgcat
     4861 gaaagaatga tattttaatg agcattctga aaaaatatcg atgcatttcg agcgaagatg
     4921 gtgaggatcc ctgaatgcgt ttttctcacc gactgttctt actgctgatt ctcctgctga
     4981 cgggagcacc aatactcgct caggaacctt ctgacgttgc gaaaaacgtg cgtatgatgg
     5041 tttccggtat tgtcagttac acccgctggc ctgcgctatc agggccacca aagttatgta
     5101 tcttttcatc ttcacgtttt agtaccgccc tacaagagaa tgccgcgaca tctttgcctt
     5161 atctgcctgt catcattcac actcaacaag aggcgatgat ttcaggctgc aatggttttt
     5221 attttggcaa cgagtcaccg acattccaga tggaattaac cgaacaatat ccgtcaaggg
     5281 cgttattatt aattgccgaa caaaataccg agtgcattat tggtagcgcc ttttgtctga
     5341 tcatccacaa taatgacgtc agatttgccg taaacctgga tgccttatcg cgtagcggag
     5401 taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa aaatgatgga taacgataat
     5461 tctcttaata agcgccccac gtttaaaaga gcattacgca acatcagtat gaccagcata
     5521 tttatcacta tgatgctgat ctggttgctg ctttccgtga cctcggtgct gaccctgaaa
     5581 cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa tgacttacag tctggaagca
     5641 gcggtcgttt ttgccgatgg ctctgcagca actgaaacac tggcagcgct ggggcagcaa
     5701 ggccaatttt caactgcaga agtgcgtgat aagcagcaaa atattctggc atcctggcat
     5761 tacacccgta aggatccagg cgatactttc agtaatttca taagccactg gctcttccct
     5821 gcccccatcg ttcagccgat tcgtcacaat ggtgaaacca ttggcgaagt tcgcttaacc
     5881 gctcgcgaca gttcaatcag ccatttcatc tggttttcgc tcgctgtcct gaccgcctgc
     5941 attctgctgg catcaggcat cgcaattacc ctcacccgcc atttgcacaa gggtctggtt
     6001 gaagcgttaa aaaacatcac cgatgtcgta catgatgtgc gttccaaccg caatttttcc
     6061 cgacgagttt cggaagaacg tatcgcagag tttcaccgct tcgctctcga cttcaacagt
     6121 ctgctggatg aaatggaaga gtggcagctt cgtttacagg ccaaaaatgc gcagcttcta
     6181 cgtaccgcac tacatgaccc attaaccggg ctggctaacc gcgcagcgtt tcgtagcggt
     6241 atcaacacgt tgatgaacag ttccgatgcc cgaaaaacgt cggcgttact atttcttgat
     6301 ggcgataatt tcaaatatat caatgatacc tggggtcatg cgacgggcga tagagtcttg
     6361 attgaaatcg caaaacggtt agctgaattt ggcgggctgc aacataaagc ataccgcctg
     6421 ggcggcgatg aattcgcgat ggtgctctat gatgtacagt cggaatctga agtgcagcag
     6481 atatgctcag cactgacaca aatctttaat cgcccgtttg atcttcataa tggccatcag
     6541 accaccatga cattaagcat tggttacgca atgaccattg cgcacgcttc tgcggaaaaa
     6601 ttacaagagc tggccgatca caatatgtat caggccaaac accagcgtgc cgaaaagctg
     6661 gtgagataac aaggatatat cgatatgata aagcacctgg tagcacccct gattttcacc
     6721 tcgctagtac tgactggctg ccagtcccct cagggaaagt ttactcctga gcaagtcgtc
     6781 gctatgcaat cttatggatt tactgaatcc gccggcgact ggtcgctggg cttatcagat
     6841 gccattctgt tcgcaaaaaa tgactacaaa ttgctcccgg aaagccagca acagatccaa
     6901 accatggcag ccaaattggc ctcgacaggg ctaacacatg cccgtatgga tggacacacc
     6961 gataactatg gtgaagacag ttacaacgaa ggcttatcat tgaaacgggc gaatgtcgtg
     7021 gccgatgcat gggctatggg tggaaaaatt ccacgcagca atctcaccac acagggttta
     7081 ggaaaaaaat atcccatagc cagtaacaag accgcccagg gccgcgccga gaaccgccgc
     7141 gtcgcagtgg tgattacaac cccttaaaac acgagcaaac agacaaaaaa agggccagcc
     7201 aattggccag cccttcttaa caggatgtcg cttaagcgaa atcttagtta agacgctctt
     7261 tgatacgagc agccttacca gtacgctcac gcaggtagta cagtttagct ttacgaacag
     7321 caccacgacg tttgacagaa atgctgtcaa ctaccggaga gtgagtctgg aagacacgct
     7381 caacgccttc gccgttggaa attttacgaa cagtgaatgc agagtgcaga ccgcggttac
     7441 gaatagcgat aaccacgccc tcgaatgcct gcagacgttt tttggaacct tcaacaaccc
     7501 atactttcac ttccacggta tcacccggac ggaaggaagg tacgtcctgc ttcatctgct
     7561 cttgttcaag ttgcttaata atgttgctca taatttaatc tcttatcctg ggtaaactga
     7621 tatctcgggg gcttacgcca tcccatcatg tttatgttgc tgttgtgcgt gttccgtttt
     7681 gaactccgcc agcaaccttg cttgctcttc agtcagagcc aggttttcca gaagttcagg
     7741 tcttctaagc caggtacggc ccagcgactg tttcaaacgc cagcgacgta tctcggcatg
     7801 gttgcccgac agtaacactg acggaacttc catcccttct aacacctcag gccgcgtata
     7861 gtgcgggcaa tccagcaatc cttcagcaaa ggaatcttcc gttgccgagg cttcatgtcc
     7921 cagtactccc ggaataaacc gggaaacgga gtcaatcagc gtcattgctg gtaactcacc
     7981 accactgaga acgtaatcgc cgattgacca ttcttcgtca atttcggttt ggatcacgcg
     8041 ctcatctata ccttcgtagc gaccgcacac cagaatcaat ttttgattcg ttgccagttc
     8101 gctgacgccc gcttgatcaa gcttgcgtcc ctgtggtgac agataaatca cctttgcgcc
     8161 ttcacccgcc gcggcttttg ctgcatgaat ggcgtcccgc aaaggttgca ccatcattaa
     8221 catccccggt ccgccgccgt aaggacgatc gtccacggta cggtgccggt catgcgtgaa
     8281 gtcgcgagga ctccagctct ggatgctcag caggccattt ttaactgccc ggccagttac
     8341 cccgtaatcg gtaattgcgc ggaacatttc aggaaacagg ctaattatgc caatccacat
     8401 agcgccgtct tttaccgttt atccggtggt ttaaaaacca ggatcccaat ctacttcgat
     8461 tgaacgagta gtgagatcga ctttcttgat aacctgccca tcgaggaacg gtacgagacg
     8521 ttccttgata ccaaacgcat ctttcaggtt tgccttaatg acgagaacat cattagatcc
     8581 ggtttccatc atatcgacga ctttaccgag atcgtagcct tcagtggtta ctacctggca
     8641 gcccatcagg tctttccagt agtagtcacc ctcttcaagc tgaggcagct gcgatgaatc
     8701 cacgacaatt tcacaattcg tcagtaggtt cgccgcatca cgatcgtcaa cgcctttcag
     8761 cttgatgatc atgtcctgat tgtggtgctt ccagctttcc agctggactt gctgccactg
     8821 acccgccttc tggataaacc agggctgata gtcaaaaatg ctttcggcgt cttcggtgga
     8881 agaaaacact ctgagccacc cacgaatacc gtaagacgaa cccatttttc ccaaaacgat
     8941 gggatcaaca ggtgcttgcg cggtgagttg tttgctcatc atgaccaccg tgacagatta
     9001 agctgctttg tttacttctt tgatcagcgc agcaacgcga tcagaaatag ttgcgccctg
     9061 gccaacccag tgagcgatgc gatccagatc caggcgagtg ccttcttctt tttcgctagc
     9121 gattgggttg aagaaaccaa cgcgctcgat gaagcgaccg ttgcgtgcat tacggctgtc
     9181 agcgacaaca acctggtaga acggacgctt tttagcgccg tgacgtgcta aacgaatagt
     9241 taccataaca tcctcttgtg tgaataaaac aaccggaccc catcgaggaa cggagtccgg
     9301 tgtcatatta aaagcccgaa aattttactc atttttgcgg aaattgcaat caacagttgc
     9361 taactctgct gtaaaaggcc gtcggcggtg cagccagttt ggtgccggag tgcgcgcagt
     9421 caccggagcg tacacgcagt acgtgaggat gacgagcaca ccccggtgcc aaaatggcaa
     9481 acaagccagg ccgattagcg accaggaaat cccggcggca tcatcccttt catgcttctc
     9541 atcatcttcg ccattccgcc cttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac
     9601 tgcttcagaa ggcggttaac gtcctgtacc tgcatcccgc aaccggcggc gatacgacgc
     9661 ttacgtgaac ctttgatgat ttctggctta gcgcgctctt tcatcgtcat tgagttgatg
     9721 atggcttcca tacgcaccag cactttatcg tccatctgcg acttgacgtt atccgggatc
     9781 tgccccatgc ccggcagttt gcccatcaag ctggccatgc cgcccatatt tttcatctgc
     9841 cgcagctgct caaggaagtc gttgagatcg aagccgtcgc cttttttcag cttgctggct
     9901 aatttctctg cctgcgcgcg gtcaactttg ctttcgatat cttcgatcag cgacagtacg
     9961 tcgcccatgc cgagaatacg cgacgcgatg cggtccggat ggaacggctc cagcgcctca
    10021 gttttctcgc caacaccgag gaacttgatc ggtttgccag tgatgtgacg aatagagagc
    10081 gccgcaccgc cgcgggcatc gccgtccact ttggtcaaca ctacgccggt aagcggtaac
    10141 gcttcattaa atgcttttgc cgtattggcc gcatcctgac cggtcatggc gtcaaccaca
    10201 aacagggttt caaccgggtt aatcgacgca tggacttgtt tgatctcgtc catcatcgct
    10261 tcgtcaacgt gcagacgacc agcggtatcc accagcagca cgtcgtagaa tttcagtttg
    10321 gcttctttca acgccgcgtt aacaatatca accggcttct gaccaacatc agaagggaag
    10381 aaatcaacgc ccacctgctc agccagcgtc tcaagctgtt tgattgccgc cgggcgatag
    10441 acgtcggcag aaaccaccag cactttcttc ttgtgcttct cgcgcaggaa cttaccgagt
    10501 ttaccaacgc tggttgtttt accggcacct tgcaggcctg ccatcagcac gaccgcaggc
    10561 ggttgcgcag ccaggttcag ggtctggttc tcttcgccca tcgccgcaac cagttcgtta
    10621 cggacgattt tgacgaactc ctgccccggc gtcaggctct tattaacttc atgaccaacc
    10681 gctttctctt ttacgcgatt gataaactca cgcactaccg gcagagcgac gtccgcctcc
    10741 agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt gaggcgtcca
    10801 cggccactga tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc
    10861 tctcgcctgg ggtggaaacg gttggccgca atcgcgacac atcatcagta ttttgccgca
    10921 gtataacatg aaggcgtctt tgttgttatg caacggttgg agcagcgttc acctgacgct
    10981 atactgcttc tctttcttat tgctcaaact gtcgacatca ctatgcccgt ttttgctctg
    11041 ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa
    11101 aacggcggct ggcggcgcat ggctattatt tctgcggtca ttgcgctggt ctgccacgca
    11161 atcgctctgg aagcccgcat cctgcccgac ggtgatagcg gacaaaacct cagcctgctg
    11221 aacgttggtt cattggtcag tttgatgatc tgtacggtaa tgaccattgt ggcttctcgc
    11281 aatcgtggct ggttgctgct acccattgtc tatgcctttg cgcttatcaa cctggcgctg
    11341 gcgaccttca tgcccaatga atacatcact catctggaag caacgcctgg gatgctggtg
    11401 cacattggct tatcgctctt ttcctatgcc acgctgatta tcgccgccct gtacgcgctg
    11461 caactggcgt ggattgatta ccaactgaag aacaagaagc tggcgtttaa ccaggaaatg
    11521 ccgccattaa tgagtatcga gcgtaaaatg ttccacatca cgcagattgg cgtggtgctg
    11581 ctaacgctca cgctttgcac tggcctgttc tacatgcaca acttatttag catggaaaat
    11641 atcgacaagg ccgtgctctc tatcgtggcg tggtttgtct atattgtgct gctgtgggga
    11701 cattatcatg aaggatggcg tggacgtcgc gtcgtctggt ttaacgttgc gggtgcagtc
    11761 attctgacac tggcctactt cggcagccga attgtccagc agttaatcag ctaaacccag
    11821 aaaaggagtt tcccctggaa cacatttcta ctactacgtt gatcattatt ctgatcatca
    11881 tggtggtcat ttcagcctat ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct
    11941 atcgtctgcg acatatggcg aaacagggta atcgctcggc caagcgcgtc gaaaaattgc
    12001 tgcgtaagcc agaccgcctg ataagcctgg tattaatcgg caataacctg gtcaatattc
    12061 ttgcctccgc gctcggcact attgttggga tgcgtttgta cggcgatgcg ggcgtggcaa
    12121 ttgcgactgg tgtgctgact tttgtcgtac tggtttttgc tgaggtattg ccgaaaacca
    12181 ttgccgcgct gtacccggaa aaagtcgctt atccgagtag ttttctgctg gctccgctgc
    12241 aaattttgat gatgccgctg gtctggttgc tgaatgctat cacccgtatg ctgatgcgca
    12301 tgatgggtat caaaaccgat atcgtggtta gcggctcttt gagcaaagaa gagttgcgca
    12361 ctatcgtgca tgagtcgcgc tcacaaattt cccgtcgcaa tcaggatatg ctgctgtctg
    12421 tgctcgatct ggaaaagatg accgttgatg acatcatggt gccgcgcagt gaaattatcg
    12481 gtattgatat caacgatgac tggaaatcga ttctgcgcca actatcccac tcacctcacg
    12541 ggcgcatcgt gctctaccgt gattcgctgg acgacgccat cagtatgctg cgagtacgtg
    12601 aagcctggcg gttgatgtcg gagaaaaaag agttcaccaa agaaaccatg ctgcgcgccg
    12661 cggacgagat ctattttgtc ccggaaggta cgcccctcag cacgcagttg gtgaagtttc
    12721 agcgcaacaa aaagaaagtc ggcctggtcg tcaacgaata tggagacatt caggggctgg
    12781 tgacggttga agatattctg gaagagatag ttggtgattt caccacgtcg atgtcgccaa
    12841 cacttgccga agaagtcact ccgcaaaacg acggttcggt gattattgat ggcactgcca
    12901 acgtgcggga aatcaacaaa gcctttaact ggcatctacc ggaagatgat gcccgcacgg
    12961 ttaatggcgt cattcttgag gcactggaag agatccctgt cgcaggcacc cgcgtgcgta
    13021 ttggcgagta cgatattgat attctcgacg tacaggacaa tatgattaag caggtaaaag
    13081 tttttcctgt gaaaccgctg cgcgagagtg tggc
//
LOCUS       DAPOZO010000118        13075 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPOZO010000118 DAPOZO010000000
VERSION     DAPOZO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.118"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            118..468
                     /locus_tag="ROI21_004019"
     CDS             118..468
                     /locus_tag="ROI21_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HDX5112884.1"
                     /translation="MKNLKKFIPPVKKPRLSGWLLTSVLLLGTIALVSPQQLPVVIYK
                     LALITLAAVLGYWLDRSLFPKARPGQYLKHDDRLMAEGRFPVQTGLHLVFSAALIRRA
                     LIVAAVCLAVAMGL"
     gene            471..1211
                     /locus_tag="ROI21_004020"
     CDS             471..1211
                     /locus_tag="ROI21_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDX5112885.1"
                     /translation="MNWPQITWIVCMSLKLAFEAFRVFIRTERLSVRAGTFLVHMAWV
                     FFVAMLLWCGGFFSQACAAQPPQAALQYRDDVIRNARLEWGLSAPVADFAAQLHQESG
                     WRPDAISPAGAQGLAQFMPATADWISQLIPMLSSREPFNPAWAIRALVSYDRWLWQRV
                     SAASDCERMAMTLSGYNGGLGWVQRDRRLASQKGLDSTRWFGHVATVNAGRNAASWRE
                     NRHYPQRILRELAPRYLTWGGSSCVASG"
     gene            1195..1845
                     /locus_tag="ROI21_004021"
     CDS             1195..1845
                     /locus_tag="ROI21_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDX5112886.1"
                     /translation="MWHLVKKLPWRGILLAILINAFLVGLYAMGYRSGHDSAKRDGDT
                     ALSQLQSAFDAYKTEQATLENAALRAWARRYQEQVAAGQRAEAGYLEQIAQLESRNKQ
                     LQGQINDVTQRWIDEKGKSHPIECVFTRGFVRQYNAALGYDNASVDTGHSDSVAAAGT
                     RSGTATGQPETTDTRLRDSGVSQRDVLANIIDNAGQCRRWRNQINALLDEREGLQK"
     gene            1842..2168
                     /locus_tag="ROI21_004022"
     CDS             1842..2168
                     /locus_tag="ROI21_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112887.1"
                     /translation="MTLQVEFWTVVSFLLTFMGFVGGLAKWLFSKTEERQAARFASLE
                     QALQQSASNWGELEKEFMRFKADLPLNYVRREDYIRGQTVIEAKLDALYNKLEVVQQY
                     RNTGGQ"
     gene            2168..2479
                     /locus_tag="ROI21_004023"
     CDS             2168..2479
                     /locus_tag="ROI21_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112888.1"
                     /translation="MVDITRVRRESLRWSLLVALNKTRPYTASETLLLDVSRAIYPDT
                     TPLELRRELDYLADRKMVDLEKKPSGDWFADLTRLGVDLVEYTVECGPGIARPEKYWS
                     E"
     gene            2479..3024
                     /locus_tag="ROI21_004024"
     CDS             2479..3024
                     /locus_tag="ROI21_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HDX5112889.1"
                     /translation="MARRSTIEKLPEDVRRWLERALTESGFSGYNELESLLRERGYVI
                     SKSAIHRYGQKIERRYGAIRAATEAARMLTEGAADDQDARSEAVIALIQTELFESIVQ
                     LQEAEEGEVDPKERVALLSKVAKNVATLSRASVNLKKFQSEVRARAQQAASNAEKIAR
                     KGGLSSDAVQALRREILGIAT"
     gene            3075..4616
                     /locus_tag="ROI21_004025"
     CDS             3075..4616
                     /locus_tag="ROI21_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112890.1"
                     /translation="MSPMDGRNNLAPVLPDTSALDAPPVLLPYQQRWVADTSPLKVIE
                     KSRRTGITWAEASDNVLTAASSAPAGGMNVYYIAYNQDMTVEYIQACAMWARAFNYAA
                     SEIEEGFWEEDDDDKHIRTYTIKFPDSGFRIVALSSRPSNLRGRQGIIVIDEAAFHEQ
                     LDELLKAALAMLIWGGKVRVISTHDGDDNPFNTLIGDIRAGRQGGSIHRITFREAVSE
                     GLFRRVCLRTGKEWSEASEQAWMASVYKFYGAGASEELDCIPANGGGAWLSRALIESR
                     MSADTPVLRLTCKEGYELLSDEVRFRETQDWLDEYLKPLLEALPTDARSFLGRDFGRS
                     GDLSVDYPLLQEKNLVRRVPFVLELRNVPFRQQEQITWYLMDGLPGLLGAAFDARGNG
                     AYLAEYAMQRYGSGRVQQVMPTEGWYREHMPPVKAALEDGNLVDLPKDEDTLDDLRAV
                     QVVNGVPRVPEQRSKAKADGGKRHGDSAIALALAYFASREINKGPVKASSRRRRQAAR
                     MLEGF"
     gene            4616..6112
                     /locus_tag="ROI21_004026"
     CDS             4616..6112
                     /locus_tag="ROI21_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HDX5112891.1"
                     /translation="MARGIWVSPDEFVAFSEPQKSLTAQIASRSRAIDFYGLGMYLPN
                     PDPILKAQGRDIRIYRELRTDPLVGGCIRRRKAALKSLERGLERGHASARVFRFIRDM
                     LDDLDLSRIIGEMSDAVLYGYQPCEIMWGRSVRAWAVTDIVGKPPEWFQFDTDNCLRF
                     RARDAGVEGELLSPSKFVVPAQDASYDNPYGFPDLSMCFWPVAFKKGGMKFWLRFAEK
                     FGSPWVIGKHPRGANDAEIEKLLDSMEQMVEDAVATIPDDSSIELKAADGKADSSEVF
                     RELITLSRSEISIALLGQNQTTEANSNKASAQAGLEVTADIRDADADIIQAAVNQVIR
                     TVVTLNFGDVPCPVWAMWEQETIDDTRATRDEKLTRAGLRLTPQYFKREYQLQDGDID
                     ETSPSERQNNMLPLSFAEAIDADIQAQQQLDDALDILMNGGVLNGTLEPVLAPLFKRV
                     ENGVNPTELLGELAELYPQMNTDDLQERLARILFVANIRGRLHERDNG"
     gene            6093..6914
                     /locus_tag="ROI21_004027"
     CDS             6093..6914
                     /locus_tag="ROI21_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDX5112892.1"
                     /translation="MSVTTAELAYCMTLPPKRAVSYLKSKGYQITWDWEEMWQEAHAR
                     AFTVAKVTRLDILEDIRGALQQAVDEGKTDRWFRQELEPVLKRKGWWGPRDTTDPVTG
                     KPVTIQQGSPWRLDTIFRTNMSVLYSAGRWAEQMENVDDRPYWMYTGINDSHTRRSHL
                     ALHGLVLRWDDPFWQAFYPPNGWRCRCSVIALSAADVRARGLKVISSGSAMGQELKLV
                     SEKTGEMRNVATFNTGTTKVTTDVGWSYAPGAAYRPDLARYQGTLQPLAQQELRG"
     gene            6917..7375
                     /locus_tag="ROI21_004028"
     CDS             6917..7375
                     /locus_tag="ROI21_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDX5112893.1"
                     /translation="MASDNLVSITINDKSLRRSLRALDLAATDLEPAMRKIAGTLLAE
                     TQFNFLDEGRPGWIPSLAAEERDGQTLQDTGRLMGSVSTDHDDRQAVVGTNVVYGAIH
                     QFGGKTGRNESVELPARPFLPVTGDGELQPEVVIPILDTIVRHLESAARR"
     gene            7590..8705
                     /locus_tag="ROI21_004029"
     CDS             7590..8705
                     /locus_tag="ROI21_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDX5112894.1"
                     /translation="MSAIHIFKAGTHTDMHGKKLPFTPDDLAACVKAYDPSVHEAPLV
                     IGHPRTEDPAWGWVKALSLSGVDLMAEPAQLDPQFAEMVTDGRFKKVSASFYLPDSPS
                     NPKPGVLYLRHVGFLGAQPPSVKGLKQVSFSEQEEGVVEFADWQAITNASLWGKLRDF
                     LIARFSLDEAEKVLPEWQLNSLREEAYRDTLSQDAAGAQFSETGPGPSSASNEESSMT
                     KEEIEALQEENRRLKQQAADRDARDAQVRQEQLHKDNVAFAEKLVAEGRLAPRASSVV
                     VALLDAVAGGDKPVEFAEGESRTPLATAFRSLLSDGEPVMNFAEQATKERVGDTVKVD
                     VAEFAEADPERLALHQKAVALSKKEGISYEAAVARCL"
     gene            8720..9673
                     /locus_tag="ROI21_004030"
     CDS             8720..9673
                     /locus_tag="ROI21_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112895.1"
                     /translation="MSDYLKGKRVVDPVLTSIARGYKNAAFIGERIFPVVLTDKEGVR
                     VPTFGKTAFVEYDTERAVGADSNVLVREKTGTLDLVLGEHDLAAPVDYREQAESMFNE
                     ESKAIRRATNGVNLRRELIAARLAQDEKVYRTGHVKKLTASDRWAGGKGDPIGVIEAG
                     MEAVRTATGLRPNLMTMGAGVMALLKFHPAIQAAIGANERKRITTEILQDLFQIEEIV
                     IGAPVSLPSMKAAMDKNSVPADIWGDNLMLHYVGKPQPGADSADENEPSFGYTLRRKG
                     MPVADKYDGAGGKVKYCRYTDIYKVAVVGGDAGYLITGISK"
     gene            9683..10021
                     /locus_tag="ROI21_004031"
     CDS             9683..10021
                     /locus_tag="ROI21_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDX5112896.1"
                     /translation="MGTTQQVILITTVTAGAALAQQRFVGADNTPCKAGAAALGVAEV
                     DAVTGDSTPVSVLGIIAVEAGAAVSRGVAVQSDAQARAVPQSGDGKSCGIALDEAGGE
                     GDVIRILRGV"
     gene            10023..10469
                     /locus_tag="ROI21_004032"
     CDS             10023..10469
                     /locus_tag="ROI21_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDX5112897.1"
                     /translation="MYCTLEDLLAQVPERTLIELTSEEMDFDSPATVNTRVVDSCIRY
                     ADELIDAHLRGRYILPLAEIPTVLRDIAITLVRYRLYARRPEGDLPDTVKDDHKEALR
                     QLRELRDNRLTLGLPSTQKDVPEPGEFRVRSRPATFGGRDGLLEKY"
     gene            10469..10933
                     /locus_tag="ROI21_004033"
     CDS             10469..10933
                     /locus_tag="ROI21_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HDX5112898.1"
                     /translation="MNVLPVLDAVLARLREKLPQLQVEYFPEKPAEYRLNHPVGALLL
                     SYAGSRFDRPDDTGAVIQSQTIQLCVTVVFRQLNGKKGAINVLDAVRRILGGHTPPGC
                     RRRIWLTREVFIGEVRGLWQYALDFATESVFIEDSDLPSGPLLTEVNYEESE"
     gene            10933..11184
                     /locus_tag="ROI21_004034"
     CDS             10933..11184
                     /locus_tag="ROI21_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112899.1"
                     /translation="MKEYRYSGPASGVTLSDGTEILLWPGKTVSLPEEHDYVKVLVAL
                     KHLTPVPEETKPAGTPAVQSPKRRSSSDSEVKTEDTHGS"
     gene            11174..12601
                     /locus_tag="ROI21_004035"
     CDS             11174..12601
                     /locus_tag="ROI21_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDX5112900.1"
                     /translation="MAANYLHGPETIEVENGARPVKTVKSAVIGLIGTAPMGDVNTLV
                     QCLSEKDAAAFGSQFTGFTIPQALDAIYDHGAGTVLVINVLDPAKHKTAIEDEVVTFD
                     KSTGQARLAHPVVANVVVKNSEGSTTHTANTDYRVDAQAGVLTNLGKAIEAGGSVKVS
                     YEYADPSKVTAADIIGGVNSAGNRTGMKLLNDSFNLYGYFAKILIAPVFCTQKSVAVE
                     LIAMAEKLGAVTYIDAPVGTTFAQALAGRGPEGTINFNTSSDRVRLCYPHVKVYDPVT
                     NTERLEPLSQRAAGLRARVDLDKGYWWSSSNQEILGITGVERQLSAMIDDPQSEVNLL
                     NEQGITTVFSSYGSGLRLWGNRTAAWPTVTHMRNFENVRRTGDVINESIRYFSQQYID
                     MPITQALIDALTESVNAYGRKMTGDGAVLGFRCWFDPARNPETELAAGHLLLSYKYTP
                     PPPLERLTFETEITSEYLLTLKGGN"
     gene            12598..>13075
                     /locus_tag="ROI21_004036"
     CDS             12598..>13075
                     /locus_tag="ROI21_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDX5112901.1"
                     /translation="MMSKIEINRITNANIYLDGTNLLGRAEEVKLPDVSMIMQEHKAL
                     GMVGKVELPAGFDKLEGEIKWNSFYRDAMLSAANPYRSLALQCRSSVQRYSSQGLIDE
                     IPLVTFLTIMFKKNPLGTFKQHENAEFSSSFTCTYIRQVLDGEELLQLDYLANIFRV"
BASE COUNT         2847 a         3569 c         3965 g         2694 t
ORIGIN      
        1 ctgtgaataa ttcatcgttt atcctgcaca tcccgtatcc ccggctctct taacgcgctt
       61 taaaatcctt tgcgccctgt atttgtgatg ctgtctccac caaacgagga gacacacatg
      121 aaaaacctga aaaaattcat tccccctgtt aaaaaacctc gcctcagcgg ctggctgctg
      181 acctcagtgc tgttgctggg caccatcgct ctggtctcac cacagcagct gcctgttgtg
      241 atctacaagc tggcactcat cacgctggca gcagtgctgg gttactggct tgaccgttcg
      301 ctcttcccca aagcccgtcc cggtcagtac ctgaaacatg acgacaggct gatggctgaa
      361 gggcgtttcc cggtacagac cggccttcac ctggtgtttt ctgctgcgtt aatccgccgt
      421 gcactgattg ttgcagcggt ctgcctggct gttgcgatgg ggctttaatc atgaactggc
      481 cgcagatcac ctggattgta tgcatgtccc tgaaactggc tttcgaagcc tttcgggtct
      541 tcatcagaac cgaacgcctg tcagtccgcg ccgggacctt tctggttcat atggcatggg
      601 tattctttgt cgcaatgttg ctctggtgtg gcggcttttt cagccaggca tgcgcagcac
      661 aacctccgca ggctgcgctg cagtatcgcg atgatgtgat ccgtaatgcc cggcttgaat
      721 ggggactgtc tgcgccggtg gccgatttcg ccgcgcaact gcatcaggaa agcggctggc
      781 gacctgatgc gatctcgccg gctggcgctc agggactggc gcagttcatg cctgccactg
      841 ccgactggat aagccagttg ataccgatgc tgagcagtcg tgagccgttt aatccggcat
      901 gggctatccg ggcgctggtc agctatgacc gctggctgtg gcagcgtgtc agcgccgcca
      961 gtgactgcga gcgtatggcc atgacactgt cgggctataa cggtggtctg ggctgggtac
     1021 aacgggacag gcggcttgca tcacagaaag gtctggacag cacccgctgg ttcggacatg
     1081 tcgccacggt gaatgccgga cgcaatgcgg ccagctggcg ggagaaccgc cattatccgc
     1141 agcgcatcct gcgcgaactg gcaccgcgat atctcacatg gggaggcagc agttgtgtgg
     1201 catctggtta aaaagctgcc gtggcgcggc attctgctgg ccattcttat caatgccttt
     1261 ctggtcggcc tgtatgccat gggatacaga agtggtcatg actctgcaaa gcgtgacggt
     1321 gataccgcgc tcagtcagtt gcagtcagca tttgacgcgt acaaaacgga gcaggcaacg
     1381 cttgagaatg ctgcgctgcg ggcctgggcc agacggtatc aggagcaggt ggccgccggg
     1441 cagcgggctg aagccggtta tcttgagcag attgctcaac tggagagccg gaataaacaa
     1501 ctacaggggc aaattaacga tgtcacacag cgctggattg atgaaaaagg taagagccat
     1561 cccattgagt gcgtgtttac tcgcggtttc gtgcgccagt acaacgccgc actcggatat
     1621 gacaacgcat ccgtcgacac cggtcattca gactcagttg ccgccgctgg cacccgctct
     1681 ggcacagcga ccgggcaacc tgaaaccact gacacccggt tacgcgattc gggtgtctcc
     1741 cagcgtgacg ttctcgccaa catcatcgac aacgcaggac aatgtcgtcg ctggcggaac
     1801 cagataaacg cgttactgga tgaacgggaa ggattacaga aatgacactg caggttgaat
     1861 tctggacggt ggtgagtttt ctgctcacct tcatggggtt tgtgggaggg ctcgccaaat
     1921 ggttgttcag taaaacagaa gaacgccagg cggcacgatt cgcctccctt gaacaggccc
     1981 tgcaacaatc cgcctccaac tggggcgagc tggaaaaaga atttatgcga tttaaagcgg
     2041 atttaccgct gaattatgtc cgtcgagagg attatatccg tggccagaca gtcattgagg
     2101 ccaaactgga cgcgctttat aacaaactgg aagtggtaca gcagtaccgc aataccggag
     2161 gtcaataatg gtcgatatta cccgggtacg ccgcgaatcc ctgcgctgga gtctgctggt
     2221 tgccctgaac aagacccgcc cttacaccgc cagcgagacg ctgctgctgg atgtgtcccg
     2281 tgccatctac ccggacacca caccgctgga actgcgccgc gaactggatt atctggctga
     2341 ccgtaaaatg gttgatctgg agaaaaaacc ttctggcgac tggtttgctg acctgacccg
     2401 cctcggcgta gacctggtgg aatacaccgt ggaatgtggt ccgggtattg cccgcccgga
     2461 aaagtactgg agtgaataat ggccagacgc agcacaatag aaaagctgcc ggaagatgtg
     2521 cgccgctggc ttgaacgggc gctgactgaa tccggcttca gcgggtataa cgagctggag
     2581 tccctgctgc gtgagcgggg gtacgtcatc agcaaatccg ctatccatcg ctatggacag
     2641 aagattgagc gccgctatgg tgctatccgt gcggcaacag aagcggcccg catgctgacc
     2701 gaaggcgcag cagacgatca ggatgcgcgt tcggaggctg tgatagccct tattcagacc
     2761 gagctgttcg agagtattgt ccagttgcag gaggcggaag aaggcgaagt cgatcctaaa
     2821 gaacgcgtgg ccctgctgtc gaaggtggcg aagaatgtgg ctacgctgtc ccgcgcgtcc
     2881 gtcaacctca aaaagttcca gtctgaagta cgggccagag cgcagcaggc agccagcaac
     2941 gccgagaaaa ttgcccgtaa ggggggactg tcaagcgacg cagtacaggc gcttcgtcgc
     3001 gagattctgg ggattgccac atgacaaaat catccggagt gattttaaac gccgctggcg
     3061 gtggccccga agggatgagt cccatggacg ggaggaataa ccttgctccc gttttgcctg
     3121 atacctcggc gctggatgct cctcccgttc tgttgcctta ccagcagcgc tgggtggcag
     3181 acacctctcc gcttaaggtg atagaaaaga gccgtcgtac cggtattaca tgggctgagg
     3241 catccgataa cgtactgacc gccgcctctt ctgcgccagc aggcgggatg aatgtgtatt
     3301 acatcgctta taaccaggac atgaccgtcg aatacattca ggcgtgtgcg atgtgggcac
     3361 gggcattcaa ctatgcggcc agtgaaattg aagaaggatt ctgggaagag gacgacgacg
     3421 acaaacacat caggacttac accatcaaat ttcctgactc cggctttcgt attgttgcgc
     3481 tctccagccg cccgtctaac ctgcgtggcc gtcagggtat tattgttatc gacgaagcgg
     3541 cgttccatga gcaactggac gaactgctga aagcggcgct ggcgatgctt atctgggggg
     3601 gaaaggtacg cgttatctcc acccatgacg gtgacgacaa tccgttcaat acgcttatcg
     3661 gggatatccg tgccggacgt cagggaggca gcatacatcg catcactttc cgggaagccg
     3721 tatctgaggg gctgttccgg cgcgtctgtc tgcgcaccgg gaaggaatgg tcggaggcat
     3781 ccgagcaggc ctggatggca tcggtgtaca aattctacgg tgccggcgca tccgaagagc
     3841 ttgactgtat tccggccaac ggtggcggtg cctggctgtc ccgtgccctg atagagtccc
     3901 gcatgtccgc tgatacgccg gtattgcgtc tgacctgcaa ggaaggttat gaactgctgt
     3961 ctgatgaggt tcgcttccgc gagacgcagg actggcttga tgagtatctg aaacccttgc
     4021 tggaggcact ccccactgat gcccgctctt tcctggggcg cgactttggc cgtagcggtg
     4081 atttgtcggt ggactatccc ctgctgcagg agaagaacct ggtacgacgc gtgccattcg
     4141 tactggagct gcgtaacgtg ccgttcagac agcaggagca aatcacctgg tatctgatgg
     4201 atggcctgcc cggtctgctg ggtgcagcgt ttgatgcccg tggtaatggt gcctatctgg
     4261 ctgaatacgc catgcagcgc tacggctccg gccgggttca gcaggtgatg ccaaccgaag
     4321 gctggtaccg ggagcatatg cctccggtca aagctgcact ggaagacggt aacctggtgg
     4381 acttaccaaa ggatgaagac acactggatg acctgcgggc cgttcaggtg gtgaacggtg
     4441 tcccccgcgt accggagcaa cgctcaaaag caaaggctga cggtggtaaa cgccacgggg
     4501 attcagccat cgcactggcg ctggcgtatt tcgccagccg tgaaattaac aaagggccgg
     4561 tgaaggcaag ctcacgccgt cgtcgtcagg cggcccgtat gctggaggga ttctgatggc
     4621 gaggggtatc tgggtttcac ccgatgaatt tgttgctttt tctgagcctc agaaatcact
     4681 gaccgcgcag attgcctccc gcagccgcgc gatcgacttt tacggactgg gcatgtatct
     4741 gcccaatcct gatcccattc tcaaggctca gggacgggat atccgtatct accgcgaact
     4801 gcgcaccgac ccgctggttg ggggctgtat ccgcagacgt aaagcagcgc tcaaatcact
     4861 ggagcgtgga ctggagcgcg gtcacgcttc tgcccgggtc ttccgtttca tccgcgacat
     4921 gctcgacgat ctggatctgt cccgcatcat cggtgagatg agtgatgccg tgctctacgg
     4981 gtatcagccc tgtgaaatca tgtggggccg ttcggtcagg gcgtgggcag tgacggatat
     5041 tgtcggcaaa ccgcctgagt ggtttcagtt tgatacggac aactgcctgc gcttccgggc
     5101 gcgtgatgcg ggtgtggagg gtgagctgct gtcaccgtca aaattcgtgg tgccggcaca
     5161 ggatgcctcg tatgacaatc cttacggttt cccggacctg tccatgtgct tctggccggt
     5221 cgccttcaag aaaggcggga tgaaattctg gctccgcttt gccgaaaagt ttggctcccc
     5281 gtgggtgatc ggtaagcacc cgaggggtgc aaatgatgca gagattgaaa aactgctgga
     5341 ctccatggag cagatggtgg aggacgcggt ggccaccatc cccgatgaca gcagcatcga
     5401 actcaaagcc gcggatggta aggcggacag cagcgaggta ttccgcgagc tgatcacact
     5461 gtcacgcagt gagatctcca ttgcattact tggtcagaat cagaccacgg aagcgaacag
     5521 taacaaggcc tctgcacagg ccgggctgga ggtaacggct gatatccgcg atgcggatgc
     5581 ggacatcatt caggcagcag tgaatcaggt tatcagaacg gtggtcaccc tgaacttcgg
     5641 cgatgtgccg tgtccggtct gggccatgtg ggaacaggag accattgatg acacccgcgc
     5701 cacccgcgac gaaaaactca cccgggcggg tctgcgtctg accccgcaat actttaagcg
     5761 tgagtaccaa ctgcaggacg gcgatattga cgagacatca ccgtcggaac gccagaataa
     5821 catgctgccg ctgtcatttg ccgaggcgat tgatgccgat attcaggctc agcagcagct
     5881 tgacgacgcg ctggacattc tgatgaacgg aggtgtgtta aatggcacgc tggaacccgt
     5941 cctggcacct ctgtttaagc gggtcgaaaa cggggttaac ccgactgagc tgctgggcga
     6001 actggcggag ctctaccctc agatgaacac ggacgatctg caggaacggc tggcccgtat
     6061 tctctttgtg gcaaatatcc ggggacgtct gcatgagcgt gacaacggct gaactggcgt
     6121 actgcatgac gcttcccccg aagcgggcag tcagttacct gaagtccaaa gggtatcaga
     6181 ttacctggga ctgggaagaa atgtggcagg aggcccatgc ccgcgccttt accgttgcta
     6241 aagtgacccg cctggatatc ctggaagata ttcgcggggc actgcagcag gctgtcgatg
     6301 aaggaaaaac cgatcgctgg ttccggcagg agctggagcc ggtgctgaag cgtaagggat
     6361 ggtggggacc acgtgacacg actgacccgg taacgggtaa gccggtcacc attcagcagg
     6421 gcagtccgtg gcggctcgat accatctttc gcaccaatat gtccgtactc tacagcgccg
     6481 gtcgttgggc ggaacagatg gaaaacgtcg acgacaggcc gtactggatg tataccggca
     6541 tcaacgacag ccatacccgc aggagccatc tggcgctgca tggtctggtg ctgcgctggg
     6601 atgacccgtt ctggcaggca ttttacccgc cgaacggctg gcgctgccgc tgtagtgtga
     6661 ttgccctgag tgcggcggat gtacgtgccc gtggcctgaa ggttatcagc tccggctctg
     6721 ccatgggcca ggaactgaaa ctggtctcag agaaaaccgg cgaaatgcgg aacgtggcca
     6781 cctttaatac cggcaccacg aaggtgacca ccgacgtcgg ctggtcttat gcaccggggg
     6841 cagcataccg tcccgacctg gcccgctatc agggtacgct tcagccactg gcacaacagg
     6901 aactgagagg ataacaatgg cttccgataa cctggtcagt atcaccatta acgataaatc
     6961 cctgcgccgg agcctccgtg cgctggatct tgctgccaca gacctggagc ccgcgatgcg
     7021 caaaatcgcc ggaaccctgc tggcggaaac acagtttaac tttcttgatg aggggcgtcc
     7081 ggggtggatc ccctcgctgg cagcggaaga acgtgacggg cagacactgc aggataccgg
     7141 gcgtctgatg gggtcagtat caaccgacca tgacgaccgg caggctgttg tggggaccaa
     7201 cgttgtttac ggtgccattc accagttcgg gggtaaaacg gggcgtaatg agtctgttga
     7261 acttccggcc cgcccgttcc tgccggtgac gggggatgga gaactacagc ctgaagtggt
     7321 aatccccatc ctcgatacca ttgtccgcca tcttgaatca gcggcccgtc gctgagtttt
     7381 ctctcttcag gcgggtgatt tatcattgcc agcgaatgag gggctgtatt acctttataa
     7441 aggctttaca gcctctgttt tataaccgcc tccggttcac cgcattgctt tccctgtcct
     7501 tctcccctga tgttttctaa agcagattaa aatcgccggg cctgcatttc tcacaaactg
     7561 tctccgacaa cataacgcgg gacagcaaaa tgtcagccat tcacattttt aaagccggta
     7621 ctcataccga tatgcacggc aaaaaactgc cgttcacgcc agacgatctt gccgcctgcg
     7681 tgaaagccta tgacccgtcc gtccatgaag caccactcgt gattggtcat cccagaacgg
     7741 aagacccggc gtggggctgg gtgaaagccc tgtcgctcag cggcgtcgat ctgatggcag
     7801 agcctgccca gctggacccg cagtttgctg agatggtcac cgacggacga ttcaaaaaag
     7861 tgtccgcctc tttctacctc ccggattcac cgtccaatcc gaagcccggc gtgctctacc
     7921 ttcgccatgt gggctttctc ggggcacagc caccttccgt caaggggctg aaacaggtgt
     7981 ccttcagtga gcaggaagaa ggtgtggtgg agttcgccga ctggcaggcc atcacgaatg
     8041 cctccctgtg gggaaagctg cgcgattttc tgatcgcccg cttcagtctg gacgaggcag
     8101 aaaaagtcct gccggaatgg cagctcaaca gtctgcgcga agaggcgtac cgcgacacac
     8161 tgtcgcagga tgcagcaggt gcacaattca gtgagacagg cccggggccg tcttccgcaa
     8221 gtaacgagga atcatcgatg acaaaagaag agattgaagc ccttcaggag gagaaccgcc
     8281 gcctgaagca gcaggctgct gatcgcgatg cgcgtgatgc acaggtcaga caggagcaac
     8341 tgcataagga caatgtggcc tttgcagaaa aactggtcgc agagggccgt ctggctcccc
     8401 gcgcctcctc cgtggtggtt gccctgctgg atgccgtcgc cggtggcgac aagccggtgg
     8461 agtttgctga gggggaaagc cgcacaccgc tggccaccgc ctttcgttca ttgctctccg
     8521 acggggagcc ggtgatgaat ttcgccgaac aggccacaaa agagcgtgtc ggcgacacgg
     8581 tgaaggtgga tgtggcagag tttgcggaag ccgatcctga gcgtctggcc ctgcatcaga
     8641 aagcagtggc cctgtccaaa aaagaaggca tcagctatga ggctgctgtc gcacgctgcc
     8701 tgtaatttaa ggagagagca tgtctgatta cttaaaaggt aaacgtgtcg ttgatccggt
     8761 actgaccagt atcgcccgtg gctataaaaa tgccgcattc atcggcgaac gtattttccc
     8821 cgtcgtgctg acggacaagg aaggcgtgcg tgtaccgacc ttcgggaaaa ccgcctttgt
     8881 ggaatatgac accgagcgtg ccgtcggggc ggacagcaat gttctggtcc gtgaaaaaac
     8941 aggcacgctg gacctggtgc tgggtgaaca cgatctggct gcgccggtgg actatcgcga
     9001 gcaggcggag tccatgttta acgaagagag caaggccatc cgtcgcgcca cgaatggcgt
     9061 gaacctgcgc cgtgaactta tcgctgcccg tctggctcag gatgaaaagg tctaccgtac
     9121 cgggcacgtc aaaaaactga cagccagtga tcgctgggcc ggtggtaagg gggaccccat
     9181 cggggtgatt gaagccggta tggaagcggt ccgtacggcc acggggctgc gtcctaacct
     9241 gatgaccatg ggggccggcg tgatggcgct gctgaagttc cacccggcga ttcaggccgc
     9301 tatcggagcc aacgaacgca agcgcatcac cacagaaatc ctgcaggacc tctttcagat
     9361 cgaagagatc gtcatcggtg ccccggtctc cctgccgtcc atgaaagcgg caatggataa
     9421 gaacagcgtg ccggcggata tctggggaga caatctgatg ctgcactatg tcggcaaacc
     9481 gcagccgggg gcggacagcg cggacgagaa cgagccgtcc ttcggctaca ccctgcgtcg
     9541 taaggggatg cctgttgccg acaaatacga cggagccggt ggcaaggtga agtactgccg
     9601 ttataccgat atctacaaag tcgccgtggt tggtggcgat gccgggtatc tcattaccgg
     9661 tatcagtaaa taaggaggcg ttatgggaac cactcagcag gtcattctga tcacaaccgt
     9721 aacggcaggg gcagcactgg cacagcagcg ttttgtcggg gcagataata ccccctgtaa
     9781 agccggtgcc gcagcgctcg gggttgccga agtggatgct gttaccggcg acagcacgcc
     9841 ggtgagcgtt ctgggcatta ttgctgtcga ggccggggcc gctgtcagcc gtggtgtggc
     9901 tgttcagtca gatgctcagg ccagagccgt gccgcagtcc ggcgacggta aatcctgtgg
     9961 tattgcactt gatgaagccg ggggtgaagg cgacgtcatt cgtatcctgc gcggggtgtg
    10021 acatgtactg caccctggag gatttgcttg cgcaggtgcc ggagcggacg cttatcgagc
    10081 tcaccagtga agagatggac ttcgactcgc ctgcaacagt gaatacccgt gtggtggaca
    10141 gctgtatccg ctatgccgac gagctgattg atgcccatct gcgcggacgc tatatcctgc
    10201 cactggcaga gataccgact gttctgcggg acattgccat cacgctggtc cgttaccggc
    10261 tctacgcccg ccgcccggaa ggtgacctcc cggatacggt gaaggatgac cacaaagaag
    10321 cgctgcggca actcagggag ttacgtgata acaggctcac gctggggctg ccgtccactc
    10381 agaaagatgt gcctgagcct ggcgagtttc gtgtacgcag tcgcccggcc actttcggcg
    10441 gtcgtgacgg tttactggag aaatactgat gaacgttctg cccgtccttg atgcggtact
    10501 ggcccggtta cgcgagaagc tgccgcaact gcaggtggag tacttcccgg agaaaccggc
    10561 tgaatatcgc ctgaaccatc cggttggcgc gttgctgttg agctatgccg gttcgcgctt
    10621 tgacaggccg gatgataccg gtgcggtgat ccagtctcag actatccagc tctgcgtcac
    10681 ggtggtcttc cgccagctca acggtaaaaa aggggcgatt aatgtcctgg atgctgtccg
    10741 ccgcattctc ggtggccaca ccccgcccgg ctgccgccgc cgtatctggc tgacccgcga
    10801 ggtgtttatc ggtgaagtca gggggctgtg gcagtacgcc ctcgacttcg cgactgaaag
    10861 cgtctttatc gaagacagcg atttaccgtc cggcccgctg ttaaccgaag tgaactatga
    10921 ggaaagcgag tgatgaaaga ataccgctat tccggcccgg ccagcggcgt cacgctgtcg
    10981 gacggaaccg aaatcctgct ctggccgggg aagacggttt ccctgccgga ggagcatgac
    11041 tacgtgaagg tactggtggc gctgaaacat ctgacgccgg tacctgaaga gactaaaccc
    11101 gccggcacac cggctgtgca gtcaccaaag cgcagaagca gcagtgacag cgaagtgaaa
    11161 acggaggaca cccatggcag ctaactatct gcatggtccc gaaaccattg aggtggaaaa
    11221 cggagcccgc ccggttaaaa cggtgaaatc tgccgttatt ggcctgattg gtaccgcccc
    11281 gatgggggat gtcaatacgc tggtacagtg cctgtctgag aaagacgcag cggcatttgg
    11341 cagccagttc accggcttta ccattccgca ggcgctggat gcgatttatg accatggtgc
    11401 aggtaccgtt ctggtcatta acgtcctcga cccggcgaaa cataaaacgg cgatcgagga
    11461 tgaggtggtt acctttgaca aatcgacagg gcaggccaga ctggcgcatc cggttgtcgc
    11521 taatgtggtg gtgaaaaaca gtgaaggcag caccacccac acggcgaaca cggactaccg
    11581 tgttgatgcg caggcgggtg tgctcacgaa cctgggcaag gctattgagg ccggtggcag
    11641 cgtgaaggtg agctatgagt acgcggaccc gtcgaaggtg actgcggcgg acatcatcgg
    11701 cggggtgaat agcgccggaa accgaactgg catgaagctg cttaacgaca gcttcaacct
    11761 gtacggctat ttcgccaaaa ttctgattgc gccggtgttc tgcacccaga agagtgtcgc
    11821 agttgagctt atcgccatgg cagagaagct gggcgcggta acctacattg atgcgcctgt
    11881 cggtaccacc tttgcacagg ctctggcagg tcgtggcccg gaaggcacca tcaacttcaa
    11941 taccagctcc gaccgcgtcc gtctgtgcta tccgcatgtg aaggtatatg acccggtgac
    12001 aaacacagag cgtctggagc cgctcagcca gcgtgcagca ggtctgcgtg ccagagtcga
    12061 tctggacaag ggctactggt ggtcatcctc caatcaggag attctgggga tcaccggcgt
    12121 ggagcgccag ctgtccgcga tgattgatga cccgcagagt gaggtgaacc tgcttaacga
    12181 acaggggatc accacggtat tcagcagtta cggcagcggc cttcgtctgt ggggtaaccg
    12241 gacggcagca tggccaacgg tcacccatat gcgtaacttt gagaacgttc gccgcaccgg
    12301 tgatgtgatc aacgagtcca ttcgttattt cagccagcag tacatcgaca tgccgattac
    12361 tcaggcgctg attgatgcac tgacggagtc ggtcaacgcc tacggtcgca aaatgactgg
    12421 tgatggtgcg gtactgggct tccgttgctg gtttgatccg gcccgcaatc cggagacgga
    12481 gctggccgcc gggcacctgt tgctgagcta caaatatacg ccaccaccgc cgctggagcg
    12541 actgacgttt gagactgaga tcacctcgga atacctgtta accctgaaag ggggcaactg
    12601 atgtcaaaga ttgagataaa ccgcatcacg aatgccaaca tctatctgga tggtactaac
    12661 ctgctgggac gggctgagga agttaaactc cccgatgtct ccatgattat gcaggaacac
    12721 aaggcgctgg ggatggtggg taaggtggaa ctcccggctg gttttgacaa actggaaggc
    12781 gaaatcaaat ggaacagctt ttaccgcgat gcgatgctgt ctgccgcgaa cccgtacagg
    12841 tcgctggcac tgcagtgtcg ttccagcgtc cagcgctaca gctcgcaggg gctgattgac
    12901 gaaatcccgc tggtcacctt cctgacgatt atgttcaaga agaacccgct ggggacgttc
    12961 aaacagcacg agaacgccga gttctccagt agcttcacct gcacgtatat cagacaggta
    13021 ctggatggtg aagagctgct gcaactggac tatctggcca acatcttccg ggtcg
//
LOCUS       DAPOZO010000119        13030 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPOZO010000119 DAPOZO010000000
VERSION     DAPOZO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.119"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..570
                     /gene="tuf"
                     /locus_tag="ROI21_004037"
     CDS             <1..570
                     /gene="tuf"
                     /locus_tag="ROI21_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDX5112902.1"
                     /translation="AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIK
                     ETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFES
                     EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIH
                     PIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            800..1183
                     /gene="secE"
                     /locus_tag="ROI21_004038"
     CDS             800..1183
                     /gene="secE"
                     /locus_tag="ROI21_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDX5112903.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1185..1730
                     /gene="nusG"
                     /locus_tag="ROI21_004039"
     CDS             1185..1730
                     /gene="nusG"
                     /locus_tag="ROI21_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDX5112904.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1889..2317
                     /gene="rplK"
                     /locus_tag="ROI21_004040"
     CDS             1889..2317
                     /gene="rplK"
                     /locus_tag="ROI21_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDX5112905.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2321..3025
                     /gene="rplA"
                     /locus_tag="ROI21_004041"
     CDS             2321..3025
                     /gene="rplA"
                     /locus_tag="ROI21_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDX5112906.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3438..3935
                     /gene="rplJ"
                     /locus_tag="ROI21_004042"
     CDS             3438..3935
                     /gene="rplJ"
                     /locus_tag="ROI21_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDX5112907.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4002..4367
                     /gene="rplL"
                     /locus_tag="ROI21_004043"
     CDS             4002..4367
                     /gene="rplL"
                     /locus_tag="ROI21_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDX5112908.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4687..8715
                     /gene="rpoB"
                     /locus_tag="ROI21_004044"
     CDS             4687..8715
                     /gene="rpoB"
                     /locus_tag="ROI21_004044"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDX5112909.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8792..13015
                     /gene="rpoC"
                     /locus_tag="ROI21_004045"
     CDS             8792..13015
                     /gene="rpoC"
                     /locus_tag="ROI21_004045"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDX5112910.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
BASE COUNT         3232 a         3230 c         3561 g         3007 t
ORIGIN      
        1 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
       61 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      121 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      181 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      241 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      301 gaatctgaag tgtacattct gtccaaagat gaaggcggcc gtcatactcc gttcttcaaa
      361 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
      421 gaaggcgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      481 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc
      541 gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact
      601 aaaagggcat catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt
      661 gattttcttt gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa
      721 gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaat
      781 acttctgaca ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct
      841 ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg gtagcgattg tcggcaacta
      901 cctttatcgc gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc
      961 agcgggtggt gtcgcgctgt taacgacaaa aggtaaagct accgttgctt ttgcccgtga
     1021 agcgcgtacc gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac
     1081 gctgattgtg gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat
     1141 tctggttcgc ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta
     1201 aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc
     1261 tgcgtgagca tatcaaatta cacaacatgg aagatttgtt tggtgaagtc atggtaccaa
     1321 ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc
     1381 ctggctacgt cctcgttcag atggtgatga acgacgcgag ctggcacctg gtgcgcagcg
     1441 taccgcgtgt gatgggcttc atcggcggta cttccgatcg tcctgcacca atcagcgata
     1501 aagaagtcga tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa
     1561 cgctgtttga accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg
     1621 gtgttgttga agaagtggat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg
     1681 gtcgtgcgac cccggtagag ctggacttca gccaggttga aaaagcctaa cccagcgatc
     1741 aaaaaagcgg cgatttaatc gttgcacaag gcgcgagatt ggaatacaat ttcgcgcctt
     1801 ttgtttttat gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg
     1861 cgttattacc caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct
     1921 gcaggttgca gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca
     1981 gggcgtaaac atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa
     2041 aggtctgccg attccggtag taatcaccgt ttacgctgac cgttctttca ctttcgttac
     2101 caagactccg ccggcagcag ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg
     2161 taagccgaac aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca
     2221 gaccaaagct gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg
     2281 tactgcacgt tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg
     2341 catgcgtgtt atccgcgaga aagttgatgc aaccaaacag tacgacatca acgaagctat
     2401 cgctctgctg aaagaactgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa
     2461 cctcggcatc gacgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca
     2521 cggtactggc cgttccgttc gcgtagccgt atttacccag ggtgcaaacg ctgaagctgc
     2581 taaagctgca ggtgctgaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg
     2641 cgaaatgaac tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct
     2701 gggccaggtt ctgggtccgc gcggcctgat gccaaacccg aaagtgggta ctgtaacgcc
     2761 gaacgttgct gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa
     2821 aaacggcatc atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga
     2881 aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt
     2941 gtacatcaag aaagttagca tctccaccac catgggtgca ggtgttgcag ttgaccaggc
     3001 tggcctgagc gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct
     3061 tacccccacg tataatgctt aatgcagacg tatatccgag atattcgggt tgtggcaagg
     3121 cggcaactga gtgagtcggc aggagcatag ctaactatgt gactggtgcg aatgaaggaa
     3181 gccaacgccg ccacaagctg aatagcgacg gatagaaaag atttgttcgt tggagcctgg
     3241 cctatccagg cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta
     3301 gacggtgaca gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa
     3361 ttaagacgct ctctccgttt ggaggagtga agtgagttcc agagattttc tctggcaaac
     3421 atccaggagc aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag
     3481 tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg
     3541 tagataaaat gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg
     3601 ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg
     3661 cgtttgttgg tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc
     3721 tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag
     3781 gtgagctgat cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag
     3841 caattgcacg cctgatggca accatgaaag aagcttcggc tggcaaactg gttcgtactc
     3901 tggctgctgt acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc
     3961 gcttacgtat aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc
     4021 aaatcattga agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa
     4081 tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc tgtagctgct ggcccggttg
     4141 aagctgctga agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag
     4201 ttgctgttat caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc
     4261 tggtagaatc tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac
     4321 tgaaaaaagc tctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg
     4381 gttgcagcct gagaaatcag gctgatggct ggtgactttt tagtcaccag cctttttgcg
     4441 ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt
     4501 ttctatcgac gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa
     4561 tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa
     4621 tagtgttgca caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg
     4681 aaccctatgg tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt
     4741 ccacaagttc tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt
     4801 atcgagcaag atcctgaagg gcagtatggt ctggaagctg ctttccgttc cgtattcccg
     4861 attcagagct acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg
     4921 gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt
     4981 aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa
     5041 gaacaagaag tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc
     5101 aacggtactg agcgtgttat cgtttcccag ctgcaccgta gtccgggcgt cttctttgac
     5161 tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg tatcatccct
     5221 taccgtggtt cctggctgga cttcgaattc gatccgaagg acaacctgtt cgtacgtatt
     5281 gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgtg ccctgaacta caccacagag
     5341 cagatcctcg acctgttctt tgaaaaagtt atctttgaaa tccgtgataa caagctgcag
     5401 atggaactgg tgccggaacg cctgcgtggt gaaaccgcat cttttgacat cgaagctaac
     5461 ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc gccacattcg ccagctggaa
     5521 aaagacgacg tcaaactgat cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct
     5581 aaagactata ttgatgagtc taccggcgag ctgatctgcg cagcgaacat ggagctgagc
     5641 ctggatctgc tggctaagct gagccagtct ggtcacaagc gtatcgaaac gctgttcacc
     5701 aacgacctgg atcacggccc gtatatctct gaaaccttac gtgtcgaccc aactaacgac
     5761 cgtctgagcg cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt
     5821 gaagcagctg aaagcctgtt cgagaacctg ttcttctccg aagaccgcta tgacctgtct
     5881 gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt
     5941 atcctgagca aagacgacat cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt
     6001 aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc cgttggcgaa
     6061 atggcggaaa accagttccg cgttggcctg gtacgtgtag aacgtgcggt gaaagagcgt
     6121 ctgtctctgg gcgatctgga taccctgatg cctcaggata tgatcaacgc caagccgatt
     6181 tctgcagcag tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac
     6241 aacccgctgt ctgagattac gcacaaacgt cgtatctccg cactcggccc aggtggtctg
     6301 acccgtgaac gtgcaggctt cgaagttcga gacgtacacc cgactcacta cggtcgcgta
     6361 tgtccaatcg aaacccctga aggtccgaac atcggtctga tcaactccct gtctgtatac
     6421 gcgcagacta acgaatacgg cttccttgag actccgtatc gtaaagtgac cgacggtgtt
     6481 gtaactgacg aaattcacta cctgtctgct atcgaagaag gcaactacgt tatcgcccag
     6541 gcgaactcca acctggatga agaaggccac ttcgtagaag acctagtaac ttgccgtagc
     6601 aaaggcgaat ccagcttgtt cagccgcgac caggttgact acatggacgt atccacccag
     6661 caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt
     6721 gcattgatgg gtgcgaacat gcaacgtcag gccgttccga ctctgcgtgc tgataagccg
     6781 ctggttggta ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggttgct
     6841 aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa
     6901 gacgagatgt atccgggtga agcaggtatc gacatctaca acctgaccaa atacacccgt
     6961 tctaaccaga acacctgtat taaccagatg ccgtgtgtgt ctctgggtga accggttgaa
     7021 cgtggcgacg tgctggcaga cggtccgtcc accgacctcg gtgaactggc gcttggtcag
     7081 aacatgcgcg tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta
     7141 tccgagcgtg ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt
     7201 gtgtcccgtg acaccaagct ggggccagaa gagatcaccg ctgacatccc gaacgtgggt
     7261 gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt acattggtgc ggaagtaacc
     7321 ggtggcgaca ttctggttgg taaggtaacg ccgaaaggtg aaactcagct gaccccagaa
     7381 gaaaaattgc tgcgtgcgat cttcggtgag aaagcgtctg acgttaaaga ctcttctctg
     7441 cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc
     7501 gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa
     7561 gacctgtctg aagaactgca gatcctcgaa gcgggtctgt tcagccgtat ccgtgctgtg
     7621 ctggtagccg gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg
     7681 gagctgggcc tgaccgacga agagaaacaa aatcagctgg aacagctggc tgagcagtat
     7741 gacgaactga aacacgagtt cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag
     7801 ggcgacgatc tggcaccggg cgtgctgaag attgttaagg tatatctggc ggttaaacgc
     7861 cgtatccagc ctggtgacaa gatggcaggt cgtcacggta acaagggtgt aatttctaag
     7921 atcaacccga tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg
     7981 aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt
     8041 atggctgcga aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaagaagtc
     8101 gcgaaactgc gcgaattcat ccagcgtgct tacgatctgg gcgctgacgt tcgtcagaaa
     8161 gttgacctga gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa
     8221 ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg
     8281 ctgaaacttg gcgacctgcc gacttctggt cagatccgcc tgtacgacgg ccgcactggt
     8341 gaacagttcg aacgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg
     8401 gtcgacgaca agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg
     8461 ctgggtggta aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg
     8521 gaagcatacg gcgcagcata caccctgcag gaaatgctca ccgttaagtc tgatgacgtg
     8581 aacggtcgta ctaagatgta taaaaacatc gtggacggca accatcagat ggagccgggc
     8641 atgccagaat ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa
     8701 ctggaagacg agtaattctc gctcaaacag gtcactgctg tcggggtaaa acccggcagc
     8761 ggattgtgct aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc
     8821 gcagactaaa accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat
     8881 ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa
     8941 accagaacgt gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg
     9001 cctgtgcggt aagtacaagc gcctgaaaca ccgtggtgtc atctgtgaga agtgcggcgt
     9061 tgaagtgacc cagactaaag tacgccgtga gcgtatgggt cacatcgaac tggcttcccc
     9121 gactgcgcac atctggttcc tgaaatcgct gccgtcccgt atcggcctgc tgctcgatat
     9181 gccgctgcgc gatatcgaac gcgtactgta cttcgaatcc tatgtggtta ttgaaggcgg
     9241 tatgaccaac ctggaacgcc agcagatcct gactgaagag cagtatctgg acgcgctgga
     9301 agagttcggt gacgaattcg acgcgaaaat gggtgcggaa gcaatccagg ccctgctgaa
     9361 gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc
     9421 cgaaactaag cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc
     9481 tggtaacaaa ccagagtgga tgatcttgac cgttctgccg gtactgccgc cagatctgcg
     9541 tccgctggtt ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg
     9601 tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat
     9661 catcgtacgt aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg
     9721 tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgatat
     9781 gatcaaaggt aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc
     9841 cggtcgttct gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa
     9901 gaaaatggcg ctggagctgt tcaaaccgtt catctacggc aagctggaac tgcgtggtct
     9961 tgctaccacc attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga
    10021 tatcctggac gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca
    10081 ccgtctgggt atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca
    10141 cccgctggtt tgtgcggcat ataacgccga cttcgatggt gaccagatgg ctgttcacgt
    10201 accgctgacg ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat
    10261 cctgtccccg gcgaacggcg aaccaatcat cgttccgtct caggacgttg tactgggtct
    10321 gtactacatg acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc
    10381 gaaagaagca gaacgtctgt atcgctctgg tctggcttct ctgcatgcgc gcgttaaagt
    10441 gcgtatcacc gagtatgaaa aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa
    10501 agacacgact gttggccgtg ccattctgtg gatgattgta ccgaaaggcc tgccttactc
    10561 catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg
    10621 cattctcggt ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggcttcgc
    10681 ctatgcagcg cgttctggtg catctgttgg tatcgatgac atggtcatcc cggagaagaa
    10741 acacgaaatc atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc
    10801 tggtctggta actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa
    10861 cgatcgtgta tccaaagcga tgatggataa cctgcaaact gaaaccgtta ttaaccgtga
    10921 cggccaggaa gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc
    10981 gcgtggttct gcggcacaga ttcgtcagct tgcaggtatg cgtggtctga tggcgaagcc
    11041 ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact
    11101 ccagtacttc atctccaccc acggtgcgcg taaaggtctg gcggataccg cactgaaaac
    11161 tgcgaactcc ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac
    11221 cgaagacgat tgtggtaccc atgaaggtat catgatgact ccggttatcg aaggtggtga
    11281 cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa
    11341 gccgggtact gctgatatcc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga
    11401 cctgctggaa gagaactctg tcgacgcggt taaagtacgt tctgttgtat cttgtgacac
    11461 cgactttggt gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa
    11521 caaaggtgaa gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct
    11581 gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt gcggctgctg aatccagcat
    11641 ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag
    11701 cggtaaactg gttatcactt cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg
    11761 taccaaagaa agctacaaag taccttacgg tgcggtactg gcgaaaggcg atggcgaaca
    11821 ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga
    11881 agtaagcggt tttgtacgct ttactgacat gatcgacggt cagaccatta cgcgtcagac
    11941 cgacgaattg accggtctgt cttcgctggt ggttctggat tccgcagaac gtactgcagg
    12001 tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg acgttctgat
    12061 cccaggtacc gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga
    12121 agatggcgta cagatcagct ctggtgacac cctggcgcgt attccgcagg aatccggcgg
    12181 taccaaggac atcaccggtg gtctgccgcg cgttgcggac ctgttcgaag cacgtcgtcc
    12241 gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa
    12301 aggtaaacgt cgtctggtta tcaccccggt agacggtagc gatccgtacg aagagatgat
    12361 tccgaaatgg cgtcagctca acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat
    12421 ttccgacggt ccggaagcgc cgcacgacat tctgcgtctg cgtggtgttc atgctgttac
    12481 tcgttacatc gttaacgaag tacaggacgt ataccgtctg cagggcgtta agattaacga
    12541 taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg
    12601 cagctccgac ttcctggaag gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg
    12661 cgaactggaa gcgaacggca aagtgggtgc aacttactcc cgcgatctgc tgggtatcac
    12721 caaagcgtct ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg
    12781 cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa ttgcgcggcc tgaaagagaa
    12841 cgttatcgtg ggtcgtctga tcccggcagg tactggttac gcgtaccacc aggatcgtat
    12901 gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc
    12961 tgccagcctg gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt
    13021 aatccgcaaa
//
LOCUS       DAPOZO010000120        12918 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPOZO010000120 DAPOZO010000000
VERSION     DAPOZO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.120"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            55..327
                     /locus_tag="ROI21_004046"
     CDS             55..327
                     /locus_tag="ROI21_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDX5112911.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(383..646)
                     /gene="yhcN"
                     /locus_tag="ROI21_004047"
     CDS             complement(383..646)
                     /gene="yhcN"
                     /locus_tag="ROI21_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDX5112912.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(1011..1481)
                     /gene="argR"
                     /locus_tag="ROI21_004048"
     CDS             complement(1011..1481)
                     /gene="argR"
                     /locus_tag="ROI21_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDX5112913.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            1916..2854
                     /gene="mdh"
                     /locus_tag="ROI21_004049"
     CDS             1916..2854
                     /gene="mdh"
                     /locus_tag="ROI21_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDX5112914.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGANVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(2917..3984)
                     /gene="degS"
                     /locus_tag="ROI21_004050"
     CDS             complement(2917..3984)
                     /gene="degS"
                     /locus_tag="ROI21_004050"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDX5112915.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(4074..5441)
                     /gene="degQ"
                     /locus_tag="ROI21_004051"
     CDS             complement(4074..5441)
                     /gene="degQ"
                     /locus_tag="ROI21_004051"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDX5112916.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(5595..5993)
                     /locus_tag="ROI21_004052"
     CDS             complement(5595..5993)
                     /locus_tag="ROI21_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDX5112917.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6187..7314
                     /gene="zapE"
                     /locus_tag="ROI21_004053"
     CDS             6187..7314
                     /gene="zapE"
                     /locus_tag="ROI21_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDX5112918.1"
                     /translation="MQSITPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            7533..7961
                     /gene="rplM"
                     /locus_tag="ROI21_004054"
     CDS             7533..7961
                     /gene="rplM"
                     /locus_tag="ROI21_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDX5112919.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            7977..8369
                     /gene="rpsI"
                     /locus_tag="ROI21_004055"
     CDS             7977..8369
                     /gene="rpsI"
                     /locus_tag="ROI21_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDX5112920.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            8764..9402
                     /gene="sspA"
                     /locus_tag="ROI21_004056"
     CDS             8764..9402
                     /gene="sspA"
                     /locus_tag="ROI21_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDX5112921.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            9408..9905
                     /gene="sspB"
                     /locus_tag="ROI21_004057"
     CDS             9408..9905
                     /gene="sspB"
                     /locus_tag="ROI21_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDX5112922.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(9949..11316)
                     /gene="dcuC"
                     /locus_tag="ROI21_004058"
     CDS             complement(9949..11316)
                     /gene="dcuC"
                     /locus_tag="ROI21_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDX5112923.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            11696..>12918
                     /locus_tag="ROI21_004059"
     CDS             11696..>12918
                     /locus_tag="ROI21_004059"
                     /inference="COORDINATES: protein motif:HMM:NF007912.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDX5112924.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFETETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGI
                     RPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSL
                     LDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSF
                     EVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKA
                     VAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVL
                     AEIEAVHQVYTYPAP"
BASE COUNT         3273 a         3180 c         3250 g         3215 t
ORIGIN      
        1 ctatacttag cgatgaacgg cagaactcgc cgcgaaacgt gacggtggca acagatgaat
       61 atttatacct ttgattttga tgagattgag agtcaggagg atttttatcg cgacttcagc
      121 caagcctttg gtctggcgaa agataaggta cgcgatctcg actcactatg ggatgtgtta
      181 atgaacgatg tcctgccgct accacttgag attgaatttg ttcatctggg agagaaaacg
      241 cgtcgccgtt ttggcgcgtt aatattgctg tttgatgagg cagaggaaga gctggaaggg
      301 catttgcgtt ttaatgttcg tcattagcgc aaaaaaaagc ccccgaaccg ggggcaatat
      361 cgtcggacaa gacgatgagg gtttatttgt acagttcagc cgtagcgtgc caggtgtcac
      421 cgctacgagc ttccgtaatc tggtaggccg ttgcgccttt ctcttctgct tttttgttca
      481 gcatttcacg catatccatt ggcgaagacg ccacaccact tacggatacg gtcccgattg
      541 cttcacgatt ttgtgcttgt gcagcatcaa tggagtcggc agcgaatgca ccgaaagaga
      601 gaacagaaag tacgcttaat gcagcaacag tggttttgat tttcatgatt tttacctcga
      661 cataatcttt tagctgggcc tttgtttcgt gacccatatc acaaaatcaa gtatacacta
      721 attattgaac taattaatac catgctaact gtttcagttg atactaagtg ttaaaaatgt
      781 gagttttaat aacaaaaaag aattaaaaac acggttattt atttttaatt ctagtatttt
      841 caattagata atgaattttg cataatgtgc tttttttacc gtatgtattc attatgtgaa
      901 tgacatgtcg cagtaaaacg cactattcgt taaattaatc gcaggggaaa gcagggggtt
      961 gagagggata agcaacattt tccccgccgc cagaagcgac ggggcagaga ttaaagctcc
     1021 tggtcgaaca gctctaaaat cgcttcgtac aggtctttga cggtgaaacc gttagcaggg
     1081 gtggtaaaga tggtgtcatc gccagcgatg gtgcccagaa taccttctgc tttgcccagt
     1141 gagtccagca ggcgagcaat taactgtgcc gcgccagggc tggtatgaat cacgacaact
     1201 gcatcgttgt agtcgatatc cagcaccaga ttcttcaatg gactggaggt ggttggtaca
     1261 cccagttcag ctggcaggca gtaaaccatt tccattttgg cattgcgtgt acgtacagca
     1321 ccaaacttgg tcaacatccg cgagacttta gactgattaa tattgtcaaa gccttgctcc
     1381 tgcaacgcgg cgacgatttc gccctgggag ctaaatttct cttctttaag taatgcttta
     1441 aatgctttaa ctagttcttc ttgcttagcc gagcttcgca taagtcaccc gatatggtgg
     1501 ttgatacaac attattgtgc atacagatga atttttatgc aaacagtcag ccctgaagaa
     1561 ggctgaaata atgttatgaa agagcgggat tttatcaaat ttcgttattg agaaacatgc
     1621 ctgcgtcacg gcatgcaaat tctgcttaaa agtaaattaa ttgttatcaa attgatgttg
     1681 ttttggctga acggtagggt atattgtcac cacctgttgg aatgttgcgc taatgcataa
     1741 gcgactgtta attacgtaag ttaggttcct gattacggca attaaatgca taaacgctaa
     1801 acttgcgtga ctacacattc ttgagatgtg gtcattgtaa acggcaattt tgtggattaa
     1861 ggtcgcggca gcggagcaac atatcttagt ttatcaatat aataaggagt ttaggatgaa
     1921 agtcgcagtc ctcggcgctg ctggcggtat tggccaggcg cttgcactac tgttaaaaac
     1981 ccaactgcct tcaggttcag aactctctct gtatgatatc gctccagtga ctcccggtgt
     2041 ggctgtcgat ctgagccata tccctactgc tgtgaaaatc aaaggttttt ctggtgaaga
     2101 tgcgactccg gcgctggaag gcgcaaatgt cgttcttatc tctgcaggtg tagcgcgtaa
     2161 accgggtatg gatcgttccg acctgtttaa cgttaacgcc ggcatcgtga aaaacctggt
     2221 acagcaagtt gcgaaaacct gcccgaaagc gtgcattggt attatcacta acccggttaa
     2281 caccacagtt gcgattgctg ctgaagtgct gaaaaaagcc ggtgtttatg acaaaaacaa
     2341 actgttcggc gttaccacgc tggatatcat tcgttccaac acctttgttg cggaactgaa
     2401 aggcaaacag ccaggcgaag ttgaagtgcc ggttattggt ggtcactctg gtgttaccat
     2461 tctgccgctg ctgtcacagg ttcctggcgt tagctttacc gagcaggaag tggctgatct
     2521 gaccaaacgt atccagaacg cgggtactga ggtggttgaa gcgaaagccg gtggcgggtc
     2581 tgcaaccctg tctatgggcc aggcagctgc acgttttggt ctgtctctgg ttcgcgcact
     2641 gcagggcgaa caaggcgttg tcgaatgtgc ctacgttgaa ggcgacggtc agtacgcacg
     2701 tttcttctct caaccgctgc tgctgggtaa aaacggcgtg gaagagcgta aatctatcgg
     2761 caccctgagc gcatttgaac agaacgcgct ggaaggtatg ctggatacgc tgaagaaaga
     2821 tatcgctctg ggcgaagagt tcgttaataa gtaattgatt agcggataat aaaaaaccgg
     2881 agcgcagact ccggtttttt gttttgagcg cacgacttaa ttggttgccg gatattcctg
     2941 aatggtgacc tgcagcgtta actgcttatc atcacgcatc actacaaccg ggatcaccga
     3001 accagggcga atttccgcca cctgatccat cgtctccaga gcagagatgg ccggtttgtt
     3061 atccaccgaa ataatcaaat cattgacctg aatacccgca ttcgccgccg ggccgtcagg
     3121 tgacacttca ttaaccacga tcccttgcag ttgatctata ccaccgccct gcgcgtgcag
     3181 tggtgcgatc tcacgtccgc cgataccaat gtagccgcgg atcacgcgac catcgcggat
     3241 cagcttatcc ataattttgg ttgctaactg gaaaggaatc gcaaagccga tgccttccgg
     3301 cgtttcgcca tcgttactct tatcaaacga tagcgtgtta atgcccatca gttcgcccag
     3361 cgagttcacc agcgcgccgc cagagttacc gtggttaatg gaagcatcag tttgcaggaa
     3421 gttttgccgc ccggttgggt tcagaccaat tcgacccgtg gcactaataa tcccctgggt
     3481 aatggtctgc ccgaggttgt acgggttacc gatcgccagt accacgtcgc caatgtgcgg
     3541 tacgcgacgt gcattgattg gaatggtagg taaaccgcca gtggcattaa ttttaagtac
     3601 cgccagatcg gttagagagt cagatcccac cagcaatgct tcaaatacac gcccatcctg
     3661 taaggcgacg atgatctgat cggcgtcgtt gatgacgtgt ttattggtga tgatataacc
     3721 gcgttgatcc atgattacac cggatcccag ggtgcggatc tcaagctggt tgtgagagtt
     3781 ggtgttcaaa ccacggttgt aaacgttaac caccgctggt gcggcgcggc gaaccgccag
     3841 attatagctg gcaggcgtct catcggtact gtcaaattgc ggagtggaaa gcgggttaag
     3901 gctgcgcagc gaaggcatgg caaccagcag aatagcgccg acaattaatc caatcgcaac
     3961 ggaacgtaag agcttcacaa acatgatgga ggcgtcatta aaaaagggaa cggcagcagc
     4021 ataccacgag ttaaccggac atcacacgtc agcctgatgc ccggtttacg acattaacgc
     4081 atcagcagat agatgctttc attgccgcgt acaatttgca gggcgatgat ggccggtttt
     4141 gccgccagca ctttacgcat ttcagcaatc gagttcaccc gatcgcggtt gacgccaatg
     4201 atcacatcgt ctttttgcaa gcctgcctga gcagcagggc ttcctttgac aacctcatcg
     4261 atcttaatac ctttgccgcc atcttttagc tgaccatcgc tcaacgttgc accttcaagc
     4321 gctggcgtga tcatttcagc gctggccgac gaagaggtgc tggtatcgag cgtcacttct
     4381 acttccagtg gtttgccgtt acgcagcagg ccaagcttca ctttcgtgcc cggctcggtg
     4441 gttgcgatac gagagcgcaa ctcagcaaag ctattcagcg gtttgccgtt gaggctggta
     4501 ataatatcgc ccgctttgac gcccgctttt gccgagccag aacctggcaa cacttcgctg
     4561 acaaacgcgc cacgctgcac gtcaaggttg aaggctttgg cgatatcggc actcatctct
     4621 gtgcctttga tgcctaacag accgcgtttg atttcaccaa agtcgataag ctgctgcgcc
     4681 agtgttcgcg ccatattact ggggatggca aatccaatcc cgacgctccc gccaccaggc
     4741 gcaaggattg ccgtgttgat gccaattaac tcaccgttaa gatttaacag tgcaccgccg
     4801 gagttaccgc ggttaatgga agcatctgtc tggataaagt tttccagacc ttcaagattc
     4861 aacccactgc ggcctaatgc tgacacaatg ccagaggtgg cggtttgccc aaggccaaat
     4921 gggttaccga ccgccacggc aaaatcaccg acgcgcaatt tatcggagtc ggcaatagcg
     4981 atttgcgtta atttgctcgg gttttgaatt tgcaacaggg cgatatcgct ctggtcatcg
     5041 ctaccaatca gttttgcatc aaactcgcgc ccatcattga gctgaatact gattttctgt
     5101 gcctgattaa tcacatggtt gttagtcagc acatagcctt tactggcgtt gatgatgaca
     5161 ccggagccta aaccttcgaa gggttgtgca ggttgatccg gtaaatcatc accaaaaaac
     5221 tttttgaatt cttccgggat tttctgtccc tgactggccg ttccttccac ccgtacgctc
     5281 accactgccg gaagcacttt ttccagcatt ggggccagac tggggagagg ggcctgatcg
     5341 gcaacctggc ctggaatcga cgcaacggcc tgaaatgacg ccgagagagt taacccgaca
     5401 cttaacgcta atgcactcaa cagctgggtt tgttttttca ttattcctgc tctcgtacct
     5461 gaatgataag aaaagagatt caaaacagtt tgatgttatt gaattttcag acggctaaca
     5521 atgagacacc agattaaatg atagacgggg agggaaagaa gaggagggcg caatggctgc
     5581 gcccgaaaaa taaattagtc gcgcttcgcg ccagtacgca gcaggcctga cgcgccttca
     5641 gaatagtcgc gaggcatctg caccggtgcc tgatcgttgc tggcttcaga ctctgccaga
     5701 cgattacgga acgggtttgc ttcagcagac agttccggca gcaggctgct ggagcttttc
     5761 gccatgtgtt gatacagctg gcgatagtcg tgcgccatgg tatccagtaa ttccgcgctg
     5821 cgggcaaagt ggctaaccag ctcttcgcgg tactcgtcca gttcagcttt attcttttcc
     5881 agttcgtact gcaacgcctg ttgctggcgt agtttacgat taccaaaacg catggccaca
     5941 gcaccaataa tgatgccgac gactaaccca attagcgcat attcccaggt catgaacatc
     6001 tcccgttgtc ttttgtttcc gtagggtgtt ggcttcaggc tccatgcctg cggctgatta
     6061 tgccactata accgttaatt ccacagaagt ggaatcccga ctgcatatcg cgtagtgtag
     6121 aacggccttt ttttcgtcaa ccgtgaacaa cggcgcaccg attattcaag gaataacaat
     6181 aagatcatgc aaagcattac cccaacatcg caatacctga aggcgctcaa tgaaggcagc
     6241 catcaacccg acgacgttca aaaagaggcc gtcagccgcc tggaaattat ttatcaggaa
     6301 ctcatcaata gcacgcctcc agcccccagg acgagtgggc taatggcgcg ggtcggtaag
     6361 ctgtggggta aacgcgaaga cacaaagcat acgccagtgc gtggcttata tatgtggggc
     6421 ggtgtaggac gcgggaaaac ctggctgatg gaccttttct atcaaagcct gccgggagag
     6481 cggaaacagc gtctgcactt tcaccgtttt atgctgcggg tacatgaaga gctaactgct
     6541 ttgcaggggc agaccgatcc gctggaaatt attgccgatc gctttaaagc agaaactgac
     6601 gtgctctgtt ttgacgaatt ttttgtttct gatattaccg acgccatgct acttggcggc
     6661 ctgatgaaag ccctgttcgc tcgcggaata acgctggtag cgacgtcaaa tattccgccg
     6721 gacgaacttt atcgaaatgg cctgcaacgt gcgcgttttt tgcctgcaat cgatgctatt
     6781 aaacagcatt gtgatgtaat gaacgtggac gctggtgttg attatcgact gcgtacactc
     6841 actcaggcgc atctgtggct ttcgcccctc aacgatgaaa cccgggcgca aatggataaa
     6901 ctatggttgg cgctggcggg ggcgaaacga gaaaattcac cgacgttaga aatcaaccat
     6961 cggccattgg cgacaatggg cgtcgagaac cagacgctgg cggtctcttt tactacgctg
     7021 tgcgtcgacg cccgcagtca gcatgactat attgcgctct cacgcctctt tcacacggtc
     7081 atgttgtttg atgtaccagt tatgacgcgg ttgatggaga gcgaagcgcg gcgctttatt
     7141 gcgctggtgg atgagtttta cgagcgccat gtcaaattag tggtgagtgc agaagtgccg
     7201 ctgtatgaaa tttatcaggg cgatcggctg aagtttgagt tccagcgttg cctgtcacgt
     7261 ctgcaagaga tgcaaagcga agagtatctg aagcgcgagc atttagcggg ttaaaacctg
     7321 ccacaaatca caaaaagggg tcgatctttg accccgactt ctctataatc ctgcgacccc
     7381 acgttacaag aaagtttttt tcccaaaact ttttgtgtgc tggcataggc tattcgaagg
     7441 ggtaggtttg ccggactttg tcgtgtgaac ctcaacaatt gaagacgttt gggtgttcac
     7501 caacgtgtaa ctatttattg ggtaagcttt taatgaaaac ttttacagct aaaccagaaa
     7561 ccgtaaaacg cgactggtat gttgttgacg cgaccggtaa aactctgggc cgtctggcta
     7621 ctgaactggc tcgtcgcctg cgcggtaagc acaaagcgga atacactccg cacgtagata
     7681 ccggtgatta catcatcgtt ctgaacgctg acaaagttgc tgtaaccggc aacaagcgta
     7741 ctgacaaagt gtactatcac cacaccggcc acatcggtgg tatcaaacaa gcgacctttg
     7801 aagagatgat tgctcgccgt cctgagcgtg tgattgaaat cgcggttaaa ggcatgttgc
     7861 caaaaggccc gctgggtcgt gctatgttcc gtaaactgaa agtttacgcg ggtaacgagc
     7921 acaaccacgc ggcacagcaa ccgcaagttc ttgacatcta atcgggatta taggcaatgg
     7981 ctgaaaatca atactacggc actggtcgcc gcaaaagttc cgcagctcgc gttttcatca
     8041 aaccgggcaa cggtaaaatc gtaatcaacc aacgttctct ggaacagtac ttcggtcgtg
     8101 aaactgcccg catggtagtt cgtcagccgc tggaactggt cgacatggtt gagaaactgg
     8161 acctgtacat caccgttaaa ggtggtggta tctctggtca ggctggtgcg atccgtcacg
     8221 gtatcacccg cgctctgatg gaatacgacg agtccctgcg ttctgaactg cgtaaagctg
     8281 gcttcgttac tcgtgacgct cgtcaggttg aacgtaagaa agtcggtctg cgtaaagcac
     8341 gtcgtcgtcc gcagttctcc aaacgttaat tggcttctgc tccggcagaa aacaatttgc
     8401 gaaaaaaccc gcttcggcgg gtttttttat ggctaaattc tgaatcagcg taaaaaatgg
     8461 aaagttgctt tttgctgccg tctgacagac aggcaaaaca aaaaccacat cgccaataag
     8521 ggactaagtc aactatttca gactaaagcg catctctttt tccccatttc cggcatcgac
     8581 tcaccacaaa ggtcgcaaaa tctggtaaac tatcatccaa ttttctgccc aaatgtcggg
     8641 tattgctcat tttttgtttg attttcgaac aaagagagtc gttccttact gggtaacaca
     8701 acttctgact ggccacctgg tggctggtag cagtaaaaat tctgactata cctggaggtt
     8761 ttcatggctg tcgctgccaa caaacgttcg gtaatgacgc tgttttccgg tcctactgac
     8821 atctatagcc atcaggtccg cattgtgctg gctgagaaag gtgtaagttt cgagatcgaa
     8881 cacgtggaaa aggacaatcc gcctcaggat ctgattgacc tcaacccgaa tcagagcgtt
     8941 ccgaccctgg tggatcgtga gctgaccctg tgggaatctc gcatcattat ggaatatctg
     9001 gatgagcgtt tcccgcatcc gccactgatg cctgtttacc cggtagctcg cggtgaaagc
     9061 cgtctgtaca tgcatcgcat cgaaaaagac tggtacacgc tgatgaacac catcatcaac
     9121 ggttcagctt ctgaagcaga tgccgcacgt aagcaactgc gcgaagaact gctggcgatt
     9181 gcgccggtct tcggtcagaa gccgtacttc ctgagcgatg agttcagcct ggtcgattgc
     9241 taccttgctc cgctgctgtg gcgtctgccg caactgggta tcgagttcag cggcccgggt
     9301 gcgaaagagc tgaaaggcta tatgacccgc gtctttgagc gtgactcttt ccttgcttct
     9361 ttaactgaag ctgaacgtga aatgcgtctg ggccggagtt aatctgtatg gatttgtcac
     9421 agctaacacc acgtcgtccc tatctgctgc gtgcattcta tgagtggttg ctggataacc
     9481 agctcacgcc gcacctggtg gtggatgtga cgctccctgg cgtgcaggtt cctatggaat
     9541 atgcgcgtga cgggcaaatc gtactcaata ttgcgccgcg tgctgtcggc aatctggaac
     9601 tggcgaatga tgaggtgcgc tttaacgcgc gctttggtgg cattccgcgt caggtttctg
     9661 tgccgctggc tgccgtgctg gctatctacg cccgtgaaaa tggcgcaggc acgatgtttg
     9721 aacctgaagc tgcctacgat gaagatgcca gcatcatgaa tgatgaagag gcatcggcag
     9781 acaacgaaac cgttatgtcg gttattgatg gcgacaagcc agatcacgat gatgacactc
     9841 atcctgacga tgaaccaccg cagccaccac gtggtggtcg accagcatta cgcgttgtga
     9901 agtaatacta aaacaggccc tggcggcctg ttttgtcttt ttaatgactt aataaaatag
     9961 cgtgattgtg gcaatcatat tcaccacgaa accgactgcc atggggatcg ctgtccgctt
    10021 cactaccgca aaaggcgaaa cgcctgcaat tcctgacgta acgaccacca ccgcagtaat
    10081 cggcgaaaca gcgcgcgcta gcgtcgtggc aaaatgcatc ggcataatca ttacaaccgc
    10141 tggcacatgt agtccggctg cgatattcgg aataagactg gcaaatgaca taaacggcgc
    10201 attgcctgag cccatcacaa tggcacaaat ggcaatgacc agcgccataa taatcatcac
    10261 gccaataccg cccagaccag aatgctccgc acccctgata accgcatcga cagtgccaat
    10321 cgtcgttaag cctttcgcaa atatttcacc cgcgaccacc agcgttacca cgttggcaaa
    10381 ctgcgtaccc atgccgtcaa aaaacgcctg cacatcatcc attgtttcgc gcaagttatg
    10441 cttgcggaag aactcaacaa acatcgtcac cgtcaaactc agtaacatca ccaccaccag
    10501 atgcagttct gactgcatca gcccgacgtg ggcaaggaac agcgagccga gcatcaggat
    10561 taacggcatc accggtaaaa tggcgtaata gagcggcggg acattatcga gagctttttg
    10621 ctctgcctgt tcgtgattga tatttttatc ttttttgtcg aaagcgcgtt gcacgaaaaa
    10681 gtgggagatc gccaccgaga taatgacgca agaggcgacc ggaagctggt agtggaagaa
    10741 gtaagtggca attttcattc ctgcgacctg ggcagcaaaa atggagttcg tttccagaat
    10801 cccccactca atggacatcg tggttgcgat aaccgccacc gcagagaggc gacttactcc
    10861 cagactcacc agcgtcggaa ataaggtgac catcagcaac atacccagac cggaggcgct
    10921 ggtaataaac tgcgccatga tttggccgat gacgtaagtt gccgacagaa taatatacgg
    10981 cgagcgaatg agttttaacg ggcggcttaa caagctcacc atcgcgcgac ttgcccctat
    11041 gcgctccata tagcgggcat aaccgcccac tgccataatc gacagcccca atccggcaat
    11101 gcggttggac agcatgcgca ggatttcatt ataaatatcg acgatcaagt agccgctgct
    11161 tttggcggga tcgagcacac caccgaaccc taaccagaca ccgcacatca tcaggaagat
    11221 acctgcggca gccagcacca cctgaggttt gtagtttttc aggatcaaat agcccatcgt
    11281 aattaatacg atgacagata taattatgcc gaacattgtt gaactccgtg tcaaaagaaa
    11341 acggtcaatc ccataaacgg cagattgaaa acaatgatgt tatatttttt gcaaggccat
    11401 ttatggtgcg gatgtcgtgt ttttaattgt aggtgaggtg atttttcatt aaaaaatatg
    11461 cgcttatgat tattttgtaa gaacatattc ataatattca taatacttgt taatagtctt
    11521 atgaataatt caatcgggat gtttttatct gcgttacatt aatttttcgc aatagttaat
    11581 tattccgtta attatggtaa tgatgaggca caaagagaaa accccgccat ttccccctac
    11641 tttcaatcct gtgataggat gtcactgatg atgttaatca cactgacctt acagaatggg
    11701 ccttatgaac gcatttgatt cgcaaaccga agattcttca cctgcaattg gccgcaactt
    11761 acgcagccgc ccgctggcgc gtaaaaaact ctccgaaatg gtggaagaag agctggaaca
    11821 gatgatccgc cgtcgtgaat ttggcgaagg tgaacaatta ccgtctgaac gcgaactgat
    11881 ggcgttcttt aacgtcgggc gtccttcggt gcgcgaagcg ctggcagcgt taaaacgcaa
    11941 aggtctggtg cagataaaca acggcgaacg cgctcgcgtc tcacgtcctt ctgcggacac
    12001 catcatcggc gagctttccg gcatggcgaa agatttcctt tctcatcctg gcgggattgc
    12061 ccatttcgaa accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    12121 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    12181 ggatcaggcg ctggatcgga agaatatccg cgaagcgttg catgacagcc tcaagcgtct
    12241 gcagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccaa ccaactgctt
    12301 tggcaaactc ggttatagct ggacggattc tgcgcctgca gtttcgctgc tggatacgct
    12361 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    12421 aactgcattt ggcgtaatgc gctacctgca tctggcagac aaacacgatc tgccgcgtat
    12481 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    12541 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    12601 gaccgggaaa tatctcaacg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    12661 cttcacacgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatagc
    12721 cagacgtcat ggcctggatc ctgcacagat ggcgctcgct tttgtacgcc gtcaaccgtt
    12781 tgttgccagc actctgctgg gcgcaaccac gatggagcag ctgaaaacta acgtcgaaag
    12841 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta
    12901 tacttatccg gcaccata
//
LOCUS       DAPOZO010000121        12581 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPOZO010000121 DAPOZO010000000
VERSION     DAPOZO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.121"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(2..568)
                     /locus_tag="ROI21_004060"
     CDS             complement(2..568)
                     /locus_tag="ROI21_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX5112925.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            complement(958..2241)
                     /gene="gltA"
                     /locus_tag="ROI21_004061"
     CDS             complement(958..2241)
                     /gene="gltA"
                     /locus_tag="ROI21_004061"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDX5112926.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(2447..2554)
                     /locus_tag="ROI21_004062"
     CDS             complement(2447..2554)
                     /locus_tag="ROI21_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112927.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2935..3339
                     /gene="sdhC"
                     /locus_tag="ROI21_004063"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2935..3339
                     /gene="sdhC"
                     /locus_tag="ROI21_004063"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDX5112928.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            3333..3680
                     /gene="sdhD"
                     /locus_tag="ROI21_004064"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             3333..3680
                     /gene="sdhD"
                     /locus_tag="ROI21_004064"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDX5112929.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFAISGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            3680..5446
                     /gene="sdhA"
                     /locus_tag="ROI21_004065"
     CDS             3680..5446
                     /gene="sdhA"
                     /locus_tag="ROI21_004065"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDX5112930.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            5462..6178
                     /gene="sdhB"
                     /locus_tag="ROI21_004066"
     CDS             5462..6178
                     /gene="sdhB"
                     /locus_tag="ROI21_004066"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDX5112931.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEADEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            6378..9179
                     /gene="sucA"
                     /locus_tag="ROI21_004067"
     CDS             6378..9179
                     /gene="sucA"
                     /locus_tag="ROI21_004067"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDX5112932.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLLQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            9194..10411
                     /gene="odhB"
                     /locus_tag="ROI21_004068"
     CDS             9194..10411
                     /gene="odhB"
                     /locus_tag="ROI21_004068"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDX5112933.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            10505..11671
                     /gene="sucC"
                     /locus_tag="ROI21_004069"
     CDS             10505..11671
                     /gene="sucC"
                     /locus_tag="ROI21_004069"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDX5112934.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11671..12540
                     /gene="sucD"
                     /locus_tag="ROI21_004070"
     CDS             11671..12540
                     /gene="sucD"
                     /locus_tag="ROI21_004070"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDX5112935.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         3041 a         3147 c         3413 g         2980 t
ORIGIN      
        1 cttatttata atccagtacg taggtcgcat tcgccgtaac tgcgcctggt gttacgggcg
       61 tagcctgatc aatttgttcc atccacgcaa agaaatttaa cgtttgttcg ctggaggttg
      121 gtgccaggtc gatatctgga gtagcggtgc ctaaaacgat attgctttga tctttattca
      181 ttaaacgcac gccgacaccg gtcgcttcac ccgtgctggt gttgttgaga atcggatctg
      241 cgccggtggt tttcgctgag ctgtcaaagg tgactgcaac tttagaaatc ttgccgccgg
      301 aaccgttgtt ggagttttcc agatcgcagt tgactaagcg caggtcgaca gcaacggcat
      361 cgctgtgatg cgactgatta atgtggctgg tggtcacctg accgagttca acctctttat
      421 caatatcatc ctgatgtatt tcacacggtg cttcaataac ttcaccttta aagtgaattt
      481 caccggatcc ttcatcagcc gcataaacag gtgcagtgaa cagcagagat acggccagtg
      541 cggccaatgt tttttgtcct ttaaacatag ctgagtcctt taaataatga gatagagtgt
      601 tgcgaggcaa gaatatatta actgacagtt gaatgtattt ggttttattt aacaatatta
      661 aaaagcttgt tatagaaaga atggaataat tgagtgcata aatttattgt ttaatgagat
      721 gaaagatggg gtgggatgga tatgcataat tttcattggg ttaacaatgc taccggcagg
      781 aaagtataaa aataaactta tcattctgct cagtatgtga cttgtcacat tttttcatct
      841 tattaatggg tatttgcatc tggtttaatt tttacaattt attatgttca gccatataaa
      901 aaagaacccg ctctattaaa ggcgggtccg gaaagtaaac ggcttagcaa tcaaccatta
      961 acgcttaatg tcgcttttaa agtcgcgttt ttcatatcct gtatacagtt gacgcggacg
     1021 ggcaatcttc ataccgtcac tgtgcatttc gctccagtgg gcgatccagc caacggtacg
     1081 tgccatagcg aagatcacgg tgaacataga agacggaata cccatcgctt tcaggatgat
     1141 accagagtag aaatcgacgt tcgggtagag tttcttctcg ataaagtacg ggtcgttcag
     1201 cgcgatgttt tccagctcca tagccacttc cagcaggtca tctttggtac ccagctcttt
     1261 cagcacttca tggcaggttt cacgcattac ggtggcgcgc gggtcgtaat ttttgtacac
     1321 gcggtgcccg aagcccatca ggcggaaaga gtcatttttg tctttcgcac gacgaacaaa
     1381 ttccggaatg tgtttaacgg agctgatttc ttccagcatt ttcagcgccg cttcgttagc
     1441 accaccgtgc gcaggtcccc acagtgaagc aatacctgct gcgatacagg caaacgggtt
     1501 cgcccccgaa gagccagcgg tacgcacggt ggaggtggag gcgttctgtt catggtcagc
     1561 gtgcaggatc aaaatacggt ccatagcacg ttccagaatc ggattaactt catacggttc
     1621 gcacggcgtg gagaacatca tattcaggaa gttaccagca taggagagat cgttgcgcgg
     1681 gtaaacaaat ggctgaccaa tggaatactt gtaacacatc gcggccatgg tcggcatttt
     1741 cgacagcagg cggaacgcgg caatttcacg atgacgagga ttgttaacat ccagcgagtc
     1801 gtgatagaac gccgccagcg cgccggtaat accacacatg actgccattg gatgcgagtc
     1861 gcgacggaaa gcatggaaca gacgggtaat ctgctcgtgg atcatggtat gacgggtcac
     1921 cgtagtttta aattcgtcat actgttcctg cgtcggtttt tcaccattca gcaggatgta
     1981 acaaacttcc aggtagttag aatcggtcgc cagctgatcg atcgggaaac cgcggtgcag
     2041 caaaatacct tcatcaccat caataaaagt aattttagat tcgcaggatg cggttgaagt
     2101 gaagcctggg tcaaaggtga acacaccttt tgaaccgaga gtacggatat caataacatc
     2161 ttgacccagc gtgcctttca gcacatccag ttcaacagct gtgtccccgt tgagggtgag
     2221 ttttgctttt gtatcagcca tttaaggtct ccttagcgcc ttattgcgta agactgccgg
     2281 aacttaaatt tgccttcgca catcaacctg gctttacccg tttgttattt ggctcgccgc
     2341 tctgtgaaag aggggaaaac ctgggtacag agctctgggc gcttgcaggt aaaggatcca
     2401 ttgatgacga ataaatggcg aatcaagtac ttagcaatcc gaattattaa acttgtctac
     2461 cactaataac tgtcccgaat gaattggtca atacttccac actgttacat aagttaatct
     2521 taggtgaaat accgacttca taacttttac gcattatatg cttttcctgg taatgtttgt
     2581 aacaactttg ttgaatgatt gtcaaattag atgattaaaa attaaataaa tgttgttatc
     2641 gtgacctgga tcactgttca ggataaaacc cgacaaacta tatgtaggtt aattgtaatg
     2701 attttgtgaa cggcctatac tgccgccagg tctccggaac accctgcaat cccgagccac
     2761 ccagcgttgt aacgtgtcgt tttcgcatct ggaagcagtg ttttgcatga cgcgcagtta
     2821 tagaaaggac gctgtctgac ccgcaagcag accggaggaa ggaaatcccg acgtctccag
     2881 gtaacagaaa gttaacctct gtgcccgtag tccccaggga ataataagaa cagcatgtgg
     2941 gcgttattca tgataagaaa tgtgaaaaaa caaagacctg ttaatctgga cctacagacc
     3001 atccggttcc ccatcacggc gatagcgtcc attctccatc gcgtttccgg tgtgatcacc
     3061 tttgttgcag tgggcatcct gctgtggctt ctgggtacca gcctctcttc ccctgaaggt
     3121 ttcgagcaag cttccgcgat tatgggcagc ttcttcgtca aatttatcat gtggggcatc
     3181 cttaccgctc tggcgtatca cgtcgtcgta ggtattcgcc acatgatgat ggattttggc
     3241 tatctggaag aaacattcga agcgggtaaa cgctccgcca aaatctcctt tgttattact
     3301 gtcgtgcttt cacttctcgc aggagtcctc gtatggtaag caacgcctcc gcattaggac
     3361 gcaatggcgt acatgatttc atcctcgttc gcgctaccgc tatcgtcctg acgctctaca
     3421 tcatttatat ggtcggtttt ttcgctatca gtggcgagct gacatatgaa gtctggatcg
     3481 gtttcttcgc ctctgcgttc accaaagtgt tcaccctgct ggcgctgttt tctatcttga
     3541 tccatgcctg gatcggcatg tggcaggtgt tgaccgacta cgttaaaccg ctggccttgc
     3601 gcctgatgct gcaactggtg attgtcgttg ctctggtggt ttacgtgatt tatggattcg
     3661 ttgtggtgtg gggtgtgtga tgaaattgcc agtcagagaa tttgatgcag ttgtgattgg
     3721 tgccggtggc gcaggtatgc gcgcggcgct gcaaatttcc cagagcggtc agacctgtgc
     3781 gctgctctct aaagtcttcc cgacccgttc ccataccgtt tctgcgcaag gcggcattac
     3841 cgttgcgctg ggtaataccc atgaagataa ctgggaatgg catatgtacg acaccgtaaa
     3901 aggttcggac tatatcggtg accaggacgc gattgaatat atgtgtaaaa ctgggccgga
     3961 agcgattctg gaactggaac atatgggcct gccgttctcg cgtcttgatg atggtcgtat
     4021 ctatcaacgt ccgtttggcg gtcagtcgaa aaacttcggc ggcgagcagg cggcacgcac
     4081 tgcggcggct gctgaccgta ccggtcacgc actgttgcac acgctttatc agcagaacct
     4141 gaaaaaccac accactattt tctccgagtg gtatgcgctg gatctggtga aaaaccagga
     4201 tggcgcggtg gtgggttgta ctgcactgtg catcgaaact ggtgaagtgg tttatttcaa
     4261 agcccgcgct accgtgctgg cgactggcgg ggcagggcgt atttatcagt ccaccactaa
     4321 cgcccacatt aacactggcg acggtgtcgg catggctatc cgtgcaggcg taccggtgca
     4381 ggatatggaa atgtggcagt tccacccgac cggtattgca ggtgcgggcg tcctggtcac
     4441 cgaaggttgc cgtggtgaag gcggttatct gctgaacaaa catggcgaac gctttatgga
     4501 gcgttatgcg ccgaacgcca aagacctggc gggccgtgac gtggtggcgc gttccatcat
     4561 gatcgaaatc cgtgaaggtc gcggctgtga tggtccgtgg gggccacacg caaaactgaa
     4621 acttgaccat ctgggtaaag aagttcttga atcccgtctg ccgggtatcc tggaactctc
     4681 ccgcaccttc gctcacgttg atccggtgaa agagccgatt ccggttatcc caacctgtca
     4741 ctacatgatg ggcggaattc cgactaaagt gaccggtcag gcgctgacgg tgaacgagaa
     4801 aggcgaagat gtggtggttc cggggctgtt cgcggtaggt gaaattgcct gcgtatcggt
     4861 acacggcgct aaccgtcttg gcggtaactc gctgctggac ctggtggtct ttggtcgcgc
     4921 agcgggtctg catctgcaag agtctattgc cgagcagggc gcactgcgcg atgccagcga
     4981 gtctgatgta gaagcgtctc tggatcgcct gaatcgctgg aacaacaacc gtaacggtga
     5041 agatccggtg gcgatccgta aagcactgca agaatgtatg cagcataact tctcggtctt
     5101 ccgtgaaggt gatgcgatgg cgaaagggct tgagcagttg aaagttatcc gcgagcgtct
     5161 gaaaaatgcc cgtctggatg acacttccag cgagttcaac acccagcgtg ttgagtgcct
     5221 ggaactggat aacctgatgg aaacggcgta tgcaacagct gtttctgcca acttccgtac
     5281 cgaaagccgt ggcgcgcata gccgcttcga tttcccggat cgtgatgatg aaaactggct
     5341 gtgccactcc ctgtatctgc cagagtcgga atccatgacg cgccgaagcg tcaacatgga
     5401 accgaaactg cgcccggcat tcccgccgaa gattcgtact tactaatgcg gagacaggaa
     5461 aatgagactc gagttttcaa tttatcgcta taacccggat gttgatgatg ctccgcgtat
     5521 gcaggattac accctggaag cggatgaagg tcgcgacatg atgctgctgg atgcgcttat
     5581 ccagctgaaa gagaaagatc ccagcctgtc gttccgccgt tcctgccgtg aaggtgtgtg
     5641 cggttccgac ggtctgaaca tgaacggcaa gaatggtctg gcctgtatta ccccgatttc
     5701 ggcactcaac cagccgggca agaagattgt gattcgcccg ctgccaggtt taccggtgat
     5761 ccgcgatttg gtggtagaca tgggacaatt ctatgcgcaa tatgagaaaa ttaagcctta
     5821 cctgttgaat aatggacaaa atccgccagc tcgcgagcat ttacagatgc cagagcagcg
     5881 cgaaaaactc gacgggctgt atgaatgtat tctctgcgca tgttgttcaa cctcttgtcc
     5941 gtctttctgg tggaatcccg ataagtttat cggcccggca ggtttgttag cggcatatcg
     6001 tttcctgatt gatagccgtg ataccgagac tgacagtcgc cttgacggtt tgagtgatgc
     6061 attcagcgta ttccgctgtc acagcatcat gaactgcgtc agtgtatgtc cgaaggggct
     6121 gaacccgacg cgcgccatcg ggcatatcaa gtcgatgttg ttgcagcgta atgcgtaatt
     6181 aaatgttgat gccggggaaa tccccggcta ttcaggaaat ctttaaaaac tgcccctgac
     6241 actaagacag tttttaaagg ttccttcgcg agccactacg tagacaagag ctcgcaagtg
     6301 aaccccggca cgcacatcac tgtgcgtggt agtatccacg gcgaagtaag cataaaaaag
     6361 atgcttaagg gatcacgatg cagaacagcg ctttgaaagc ctggttggac tcttcttacc
     6421 tctctggcgc aaaccagagc tggatagaac agctctatga agacttctta accgatcctg
     6481 actcggttga cgctaactgg cgttcgacgt tccagcagtt acctggtacg ggagtcaaac
     6541 cggatcaatt ccactctcaa acgcgtgaat atttccgccg cctggcgaaa gacgcttcac
     6601 gttactcttc aacgatctcc gaccctgaca ccaatgtgaa gcaggttaaa gtcctgcagc
     6661 tcattaacgc ataccgcttc cgtggtcacc agcatgcgaa tctcgatccg ctgggactgt
     6721 ggcagcaaga taaagtggcc gatctagatc cgtctttcca cgatctgacc gaagcagact
     6781 tccaggagac cttcaacgtc ggttcatttg ccagcggcaa agaaaccatg aaactcggcg
     6841 agctgctgga agccctcaag caaacctact gcggcccgat tggtgccgag tatatgcaca
     6901 ttaccagcac cgaagaaaaa cgctggatcc aacagcgtat cgagtctggt cgcgcgactt
     6961 tcaatagcga agagaaaaaa cgcttcttaa gcgaactgac cgccgctgaa ggccttgaac
     7021 gttacctcgg tgcaaaattc cctggcgcaa aacgcttctc gctggaaggc ggtgacgcgt
     7081 taatcccgat gcttaaagag atgatccgcc acgctggcaa cagcggcacc cgtgaagtgg
     7141 ttctcgggat ggcgcaccgt ggtcgtctga acgtgctggt gaacgtgctg ggtaaaaaac
     7201 cgcaagactt gttcgacgag tttgccggta aacataaaga acacctcggc acgggtgacg
     7261 tgaaatacca catgggcttc tcgtctgact tccagaccga tggcggcctg gtgcatctgg
     7321 cgctggcgtt taacccgtct caccttgaga ttgtcagccc ggtagttatc ggttctgttc
     7381 gtgcccgtct ggacagactc gatgagccga gcagcaacaa agtgctgcca atcaccatcc
     7441 acggtgacgc cgcagtgacc gggcagggcg tagttcagga aaccctgaac atgtcgaaag
     7501 cgcgtggtta tgaagttggc ggtacggtac gtatcgttat caacaaccag gttggcttca
     7561 ccacctctaa tccgctggat gctcgttcta caccgtactg tactgatatc ggtaagatgg
     7621 ttcaggcacc gattttccac gtaaacgcgg atgatccgga agccgttgct tttgtgaccc
     7681 gtctggcgct cgatttccgt aacaccttta aacgtgatgt ctttatcgac ctggtatgct
     7741 accgccgtca cggccacaac gaagccgacg agccgagcgc aacccagccg ctgatgtatc
     7801 agaaaatcaa aaaacatccg acgccgcgca aaatctacgc tgacaagctg gagcaggaaa
     7861 aagtcgcgac gctggaagat gccaccgaga tggttaacct gtaccgcgat gcgctggatg
     7921 ctggcgattg cgtagtggca gagtggcgtc cgatgaacat gcactctttc acctggtcgc
     7981 cgtacctcaa ccacgaatgg gacgaagagt acccgaacaa agttgagatg aagcgcctgc
     8041 aggaactggc taaacgcatc agcacggtgc cggaagcagt tgaaatgcag tctcgcgttg
     8101 ccaagattta tggcgatcgc caggcgatgg cagccggtga gaaactgttc gactggggcg
     8161 gcgcggaaaa ccttgcttac gccacgctgg ttgacgaagg cattccggtt cgcctgtcgg
     8221 gtgaagactc cggtcgcggt accttcttcc accgccacgc ggtgatccac aaccagtcta
     8281 acggttccac ttacacgccg ctgcaacaca tccataacgg ccagggcgcg ttccgtgtct
     8341 gggactccgt actgtctgaa gaagcagtgc tggcgtttga atacggttat gccaccgcag
     8401 aaccacgcac tctgactatc tgggaagcac agttcggtga cttcgctaac ggtgctcagg
     8461 tggttatcga ccagttcatc tcctctggcg agcagaaatg gggccggatg tgtggcctgg
     8521 tgatgttgct gccgcacggt tacgaagggc agggaccgga gcactcctcc gcgcgtctgg
     8581 aacgttatct gcaactttgc gctgagcaaa acatgcaggt gtgcgtaccg tctaccccgg
     8641 cacaggttta ccacatgctg cgtcgtcagg cgttgcgcgg gatgcgtcgt ccactggtcg
     8701 tgatgtcgcc gaaatccctg ctgcgtcatc cgctggcggt atccagcctc gaagaactgg
     8761 cgaacggcac cttcctgcca gccatcggtg aaatcgacga gcttgatccg aagggcgtga
     8821 agcgcgtagt gatgtgttct ggtaaggttt attacgacct gctggaacaa cgtcgtaaga
     8881 acaatcaaca cgatgtcgcc attgtgcgta tcgagcaact ctacccgttc ccgcataaag
     8941 cgatgcagga agtgttgctg cagtttgctc acgtcaagga ttttgtctgg tgccaggaag
     9001 agccgctcaa ccagggcgca tggtactgca gccagcatca tttccgtgaa gtgattccgt
     9061 ttggggcttc tctgcgttat gcaggccgcc cggcctccgc ctctccggcg gtagggtata
     9121 tgtccgttca ccagaaacag caacaagatc tggttaatga cgcgctgaac gtcgaataaa
     9181 taaaggatac acaatgagta gcgtagatat tctggtccct gacctgcctg aatccgtagc
     9241 cgatgccacc gtcgcaacct ggcataaaaa acccggcgac gcagtcgtac gtgatgaagt
     9301 gctggtagaa atcgaaactg acaaagtggt actggaagta ccggcatcag cagacggcat
     9361 tctggatgcg gttctggaag atgaaggtac aacggtaacg tctcgtcaga tccttggtcg
     9421 cctgcgtgaa ggcaacagcg ccggtaaaga aaccagcgcc aaatctgaag agaaagcgtc
     9481 cactccggcg caacgccagc aggcgtctct ggaagagcaa aacaacgatg cgttaagccc
     9541 ggcgatccgt cgcctgctgg ctgaacacaa cctcgacgcc agcgccatta aaggcaccgg
     9601 tgtgggtggt cgtctgactc gtgaagatgt ggaaaaacat ctggcgaaag ccccggcgaa
     9661 agagtctgct ccggcagcgg ctgctccggc ggcgcaaccg gctctggctg cacgtagcga
     9721 aaaacgtgtg ccgatgactc gcctgcgtaa gcgtgtggca gagcgtctgc tggaagcgaa
     9781 aaactccacc gctatgctga ccacgttcaa cgaagtcaac atgaagccga ttatggatct
     9841 gcgtaagcag tacggtgaag cgtttgaaaa acgtcacggt attcgtctgg gctttatgtc
     9901 cttctacgtg aaagcggtgg ttgaagccct gaaacgttac ccggaagtga acgcgtctat
     9961 cgacggcgat gatgtggttt accacaacta tttcgacgtc agcatggcgg tttctacgcc
    10021 gcgcggcctg gtgacgccgg tactgcgtga tgtcgatacc ctaggcatgg cagacatcga
    10081 gaagaaaatc aaagagctgg cagttaaagg ccgtgacggc aagctgaccg ttgaagatct
    10141 gaccggtggt aacttcacca tcaccaacgg tggtgtgttc ggttccctga tgtctacgcc
    10201 gatcatcaac ccgccgcaga gcgcaattct gggtatgcac gctatcaaag atcgtccgat
    10261 ggcggtgaat ggtcaggttg agatcctgcc gatgatgtac ctggcgctgt cctacgatca
    10321 ccgtctgatc gatggtcgcg aatccgtggg cttcctggta acgatcaaag agttgctgga
    10381 agatccgacg cgtctgctgc tggacgtgta gtagtttaag cttcacctgc actgtagacc
    10441 gacgtggata gggtgtcggt ctacggttta aaagataacg attactgaag gatggacaga
    10501 acacatgaac ttacatgaat atcaggcaaa acaacttttt gcccgctatg gcttaccagc
    10561 accggtgggt tatgcctgta ctactccgcg cgaagcagaa gaagccgctt caaaaatcgg
    10621 tgccggtccg tgggtagtga aatgtcaggt tcacgctggt ggccgcggta aagcgggcgg
    10681 tgtgaaagtt gtaaacagca aagaagacat ccgtgctttt gcagaaaact ggctgggcaa
    10741 gcgtctggta acgtatcaaa cagatgccaa tggccaaccg gttaaccaga ttctggttga
    10801 agcagcgacc gatatcgcta aagagctgta tctcggtgcc gttgttgacc gtagttcccg
    10861 tcgtgtggtc tttatggcct ccaccgaagg cggcgtggaa atcgaaaaag tggcggaaga
    10921 aactccgcac ctgatccata aagttgcgct tgatccgctg actggcccga tgccgtatca
    10981 gggacgcgag ctggcgttca aactgggtct ggaaggtaaa ctggttcagc agttcaccaa
    11041 aatcttcatg ggcctggcga ccattttcct ggagcgcgac ctggcgctga tcgaaatcaa
    11101 cccactggtc atcaccaaac agggcgatct gatttgcctc gacggcaaac tgggcgctga
    11161 tggcaacgca ctgttccgcc agcctgatct gcgcgaaatg cgtgaccagt cgcaggaaga
    11221 tccgcgtgaa gcacaggctg cacagtggga actgaactac gttgcgctgg acggtaacat
    11281 cggttgtatg gttaacggcg caggtctggc gatgggtacg atggacatcg ttaaactgca
    11341 cggcggcgaa ccagctaact tcctggacgt tggcggcggc gcaaccaaag aacgtgtaac
    11401 cgaagcgttc aaaatcatcc tctctgacga caaagtgaaa gccgttctgg ttaacatctt
    11461 cggcggtatc gttcgttgcg acctgatcgc tgacggtatc atcggcgcgg tagcggaagt
    11521 gggtgttaac gtaccggtcg tggtacgtct ggaaggtaac aacgccgaac tcggcgcgaa
    11581 gaaactggct gacagcggcc tgaatattat tgcagcaaaa ggtctgacgg atgcagctca
    11641 gcaggttgtt gccgcagtgg aggggaaata atgtccattt taattgataa aaacaccaag
    11701 gttatctgcc agggctttac cggtagccag gggactttcc actcagaaca ggccattgca
    11761 tacggcacta aaatggttgg cggcgtaacc ccaggtaaag gcggcaccac ccacctcggc
    11821 ctgccggtgt tcaacaccgt gcgtgaagct gttgctgcta ctggcgctac cgcttctgtt
    11881 atctacgtac cagcaccgtt ctgcaaagac tccattctgg aagccatcga tgcaggcatc
    11941 aaactgatta tcaccatcac tgaaggcatc ccgacgctgg atatgctgac tgtgaaagtg
    12001 aaactggacg aagctggcgt tcgtatgatc ggcccgaact gcccaggcgt tatcactccg
    12061 ggtgaatgca aaatcggtat ccagcctggt cacattcaca aaccgggtaa agtgggtatc
    12121 gtttcccgtt ccggtacact gacctatgaa gcggttaaac agaccacgga ttacggtttc
    12181 ggtcagtcga cctgtgttgg tatcggcggc gatccgatcc ctggctctaa ctttatcgac
    12241 attctcgaaa tgttcgaaaa agatccgcag accgaagcga tcgtgatgat cggtgagatc
    12301 ggcggtagcg ctgaagaaga agcggctgcg tacatcaaag agcacgttac caagccagtt
    12361 gtaggttaca tcgctggtgt gactgcgccg aaaggcaaac gtatgggcca cgcaggtgcc
    12421 atcattgccg gtggtaaagg gactgcggat gagaaattcg ctgctctgga agccgcaggc
    12481 gtgaaaaccg ttcgcagcct ggcggatatc ggtgaagcac tgaaaactgt tctgaaataa
    12541 atatctgtaa taagaaatag ccctcgccgc ttccctctac a
//
LOCUS       DAPOZO010000122        12553 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPOZO010000122 DAPOZO010000000
VERSION     DAPOZO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.122"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..86
                     /locus_tag="ROI21_004071"
     CDS             <1..86
                     /locus_tag="ROI21_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDX5112936.1"
                     /translation="WSYHALFWLAIGMLGIAMICLLFIKDI"
     gene            197..1120
                     /locus_tag="ROI21_004072"
     CDS             197..1120
                     /locus_tag="ROI21_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDX5112937.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1124..1942)
                     /gene="nlpA"
                     /locus_tag="ROI21_004073"
     CDS             complement(1124..1942)
                     /gene="nlpA"
                     /locus_tag="ROI21_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDX5112938.1"
                     /translation="MKLTTHHLRAGGALLLAGILLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITENPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2152..2445
                     /locus_tag="ROI21_004074"
     CDS             2152..2445
                     /locus_tag="ROI21_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112939.1"
                     /translation="MKPTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
     gene            2747..3244
                     /locus_tag="ROI21_004075"
     CDS             2747..3244
                     /locus_tag="ROI21_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HDX5112940.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFLCIYDFQNYNYLKGYAAVCQLKKDYQKAFDMYHICLML
                     SPDNDFSLIYYMGQCQMGLKNTKMATELFNTVVTYSQNEKVKEMATAYLELLTANTEE
                     EVQTE"
     gene            3492..5273
                     /locus_tag="ROI21_004076"
     CDS             3492..5273
                     /locus_tag="ROI21_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopB/SseC family type III secretion system
                     translocon subunit"
                     /protein_id="HDX5112941.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSNKTQVDPMRLN
                     KNPKSLDNDIRTRLENKPMLAPPEMQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     TTRLHNNLDSTLLRHEMAHNKFRELSDAYSSSLDSAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVVTQKSAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CESIKNQSEVMKEINHVRENKLNETARSYTTWTKVLNIINEVVTVVTFAVSAALIVIG
                     IVAAVPSAGASFAGALALIGGIAGAVVLGVDIICQITLGTTATGWILGKLTEGLAGII
                     SKYDPTLLAICALLDALGVQQTTIDLVQDIYSSAAASIVMVTLMIGATLICSVAISEL
                     VKALSKTAAEEITKEITDSIKSTVESMIKSVSKKIMSALNFLGDKIKASSQFIMIFAK
                     ITILLEKIASGVCTIITSSMNQYAASKSADIEILQQDISNLSKTREQMLTVMQRIDKT
                     VEQEVAQMVRVLQHRTEALKFASHSIV"
     gene            5286..6416
                     /locus_tag="ROI21_004077"
     CDS             5286..6416
                     /locus_tag="ROI21_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112942.1"
                     /translation="MTNNISNINNTAHNKNLDKTTFDEQPAYETRVSQAKKILQSFAP
                     NDIKVDTNTKFISLIESVRALSPELQCKVLDGLNYAKNTLHNINQNSAIATKSSCQST
                     ESIEKTITFNILDMLDNLTMRQQNIMEAFLRVFTSMYNASAKADASMSIVFEQLVHLQ
                     SSETKNASWANFAGALAGSAINATGAVFMAGAELAKPAGKIDDIAENQEQNTDKPDSQ
                     TRIKSKEDMANAKARLAEIENQQKDLSNNKNIEDNDKQEQSSKLQKEKESITNKIKDK
                     EKINSHNLRYGITSSISHTNNLFQSIANLSQYGGTVAAEHTRAEKDLAEQAKNVMHSV
                     MSSYLDQINRDVQGRDQALNLLQQIIQTIIDTDSAIVQGIRS"
     gene            6460..7809
                     /locus_tag="ROI21_004078"
     CDS             6460..7809
                     /locus_tag="ROI21_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HDX5112943.1"
                     /translation="MINKDNNLNIDILRQQNANIPLKEASSQSEEPHQLENSKSEGNL
                     YNSASKDNNIGAMLKKDVEKLSTLVSERSPRNEDWKCQHDIQLNFVKNMLRNFPTENL
                     SEKGSALLHESYSLIKNVLQLTENVNTIDGKLKDPAKILTLHNSVRKTLANVADNTTG
                     SSAMASTKGTLNHAIASLDSSAPEVRSSATSSTLTASSSNEFKSDKYICSDIADLMSV
                     LGSDYLEIYANSVEIMSAYWQDFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHF
                     YYIDKYPNGDFHYHYNPDYVLYPPAPADKIGVPLEEAEKWCAALGLPVIPPDPKHRTP
                     SPIVEVEPQGSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAE
                     YQAWLAGYNSQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDYLRF"
     gene            8012..9709
                     /gene="eilA"
                     /locus_tag="ROI21_004079"
     CDS             8012..9709
                     /gene="eilA"
                     /locus_tag="ROI21_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HDX5112944.1"
                     /translation="MQLQHGKNNTQQYIFGDFVLKNNGILLFKNKEYHIPPKELGVII
                     LLLNADGEIVSKEEIIDKVWSASVASDESLTRCIYALRKLLHENKQCKYIETVYGRGY
                     RFTVPIVIVTDNEPVKSTTTLAVFPFRTEGSINVLKLHYELVQGLSKYAFCGLDILPA
                     SVTNEASDFSTIHQFINKTGPEYYIMGQVVHYGKNWHLFIELIHARTHKLIDHQSIDF
                     NPDNPLSVLLSQVINILIEKIPNINFKSINTQQMPSLDSAVMYMNGRMEMYRYTPESL
                     KRALVIFRDCVSTQPQNTLPYCCLAECHISLALLGLSEQKQAITTAVTSIEKALEINP
                     SNSQALGLLGLISGLKDEHSVSNVLFKQAHLLKPNSPDVYYYQSLLCFLNGDLARAFN
                     LIEKSIALEPNKMGISILKLIILYYTSPLDNAISFALNLNSQNTCNNPIITSILAMFM
                     ALKGHNDKAKSLLLKLEPEHGLDYTCVNSLYTKFLIYGASIKNDIMKLLANINTNKIN
                     GVILPLIYTVYGKKEYEKRWQQLIKENDLWSNVLLHDPRLLSVKNEFNTIGMMRTSA"
     gene            complement(9761..>12553)
                     /locus_tag="ROI21_004080"
     CDS             complement(9761..>12553)
                     /locus_tag="ROI21_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDX5112945.1"
                     /translation="ISAVTASINNSSLSRNVTFIADVSTAKIADLVVIKDGSEADGST
                     ANTLQVKVTDANGNTLAGQTVSVLAGNSATVTPTVTTKPDGTVEISVTSQTAGISAVT
                     ASINSSSQSRNVTFIADVRTAKIADLVVIKDDSVADGAMANMLRARVTDAFGNALAGQ
                     TVSVLAGNSATVAPTMTTKPDGTVEISVTSQTAGTSTVTASINNSSQSQNVTFVPGDA
                     SQLTSIVETNKSNYTVGETITITVTLRDAFDNLVTGAASQLAADGVLTVAGTDPSEMG
                     SWVESGGVYTTTRMATIASTNQHANLQLQTWSDGVTSDRYDIQSGSPAQATSTIATDK
                     NAYTAGDTITVAVTLKDAHGNLVEGGESLLSGDNVTVEGAVRSGGWSETAGVYTATWS
                     AQMAGDSHHATLKLSEWGSSKQSESYSIHSGAPVQANSAIRTDKSAYIAGEPLTVTIT
                     LRDEFGNPALGLTSEVIESYIDSFAVGGATHDAMRWVEQNNGEYTIVWTAWVAEENLV
                     ASLKLKTWATEIKSSLYGIQPGAAAKTQSTIVADKTIYIAGDSITVTVVLKDAQGNFI
                     TDGVVQLNEENVQVRNADPIQGNNWVYNGNGQYQRQYMAHFAEANLNAQLKMAGWSDA
                     NYSNNYTIKPGEVSPLGSQLRIREVLVVEGADLPVSALLVDDFGNPVDNGLDLLDDAV
                     YLQNVEKKEGEKWRYVGDGIYERTYMAYQEGENLTSFMEIKGWRIYGQPSYTILPFVE
                     VELLSVNGVKFRATDGFPETGFDGAKFTLLLTHNMKNTDYNWTAGIYGINVDSNGEVT
                     LSVLIRSEVTITGKPKNGKGNDVVFKFKIKKWFTSLGATSSNTWDIINTSCSYGQMPS
                     SLELAQRPSGGVVPRKVGTLWGEYGNLKTYGNAFSSTDYWTSTQLMGVHEKFNPETGI
                     SELGTGKSSGLCVEYY"
BASE COUNT         3803 a         2752 c         2490 g         3508 t
ORIGIN      
        1 tctggagcta ccacgcgttg ttctggctgg cgatagggat gttgggtatt gcgatgattt
       61 gcctgctgtt tattaaagat atttagctca ccagtctggg ctaagaaaaa tcggcgtggc
      121 gcaaacagag ccatcctcgc catttttgct atcatgcctg catacataaa cgacaaaaca
      181 gtatgcagag ggaaaaatgg gttccaccag aaaggggatg ctgaacgttc tgattgccgc
      241 cgtgttgtgg ggaagttcag gggtctgcgc gcaatacatc atggagcaaa gccagatgtc
      301 gtcgcagttt ttgactatga cgcgtttgat attcgccggt ttgattctgc tgacgctgtc
      361 atttgttcat ggcgataaaa tcttttctat tattaacaat cacaaagatg ccattagtct
      421 gctgattttt tccgtggttg gcgcgctaac cgtacagctc acctttttac tgaccattga
      481 aaaatcgaat gctgccacgg caacggtgct gcaattcctc tcaccgacga ttatcgtcgc
      541 ctggttctca ctggtgcgta aatcccgccc gggcattctg gttttttgcg ctattttgac
      601 atcgctggtc gggacttttt tactggtgac acacggtaat ccgacgtcat tatcaatctc
      661 tccggccgcg ttattctggg gcattgcctc ggcatttgct gcggcattct ataccaccta
      721 tccctcaacg ctaattgccc gctatggcac gttgcctgtc gtcggctgga gtatgctgat
      781 cggcggtctg attctgttgc ctttttatgc cagacaaggg acaaactttg tcgttaacgg
      841 cagtttgatt ctggcgtttt tttatttggt ggtcattggt acgtccctga catttagtct
      901 gtacctgaaa ggagcacaat taattggcgg gccaaaagcc agcattttga gctgtgcaga
      961 gccattaagt agcgcgctgc tctctttgct gttgctgggg atcacattca cattaccgga
     1021 ctggctgggc acactgctga ttctgtcatc ggtgattttg atttcaatgg attcccgtcg
     1081 ccgcgccaga aaaataaatc gtccggcgcg ggatgagtga gaattaccag ccaggcaccg
     1141 cgccaccgtt aaagatggtt tctgccgctt tagcgacttc ggatgattga taagattgca
     1201 gaaattcctt cacgttttct gcattcttat tatcttcccg tgctaccaga atattcacat
     1261 acggcgaatt cttatcttca ataaatacgc tgtcgtgcac cggagaaagc ccggtctgct
     1321 gaatgtaagt ggtgctgata atcgccacat caactttggg atcatccagt acgcgcggga
     1381 gctgcgcccc ctcgagttcc ataatctgca aatgacgagg attctcggtt atatccagcg
     1441 cggtaggtag taaccctttt ccctctttca gagtaattag cttctctttt tgcaacagta
     1501 aaagcgcgcg accaaggttg gtcgggtcgt tggggatcgc cactgtcgcg ccttctttta
     1561 tttgcgccac ggttttgatt tttttggaat aacccgccat ggggaagaca aacgtatttc
     1621 ccaccgctac cagtttataa ccatgcgcct gattatcttg ttcaagaaag ggtcgatgct
     1681 ggaagacgtt cgcatcaagc tcgccatgat ttgtcgcatc gttcggtagc aacgaaccgc
     1741 taaatcctac caattcaaca tcgaggccat atttctcttt tgccaccttt ttagcgactt
     1801 ccgcgacatc ttgttctgcg ccattaataa cgcccacttt aatgtgcttt tcatcgctgc
     1861 tgctctggtc gcaacctgcc agcagaattc ctgccagtaa taatgcgccc cccgcccgta
     1921 gatgatgtgt tgtcagtttc acttttttat ccttttttta atcgttaact gactataatg
     1981 gcaagatcac tacgatttaa aaaacgaaaa gctatcgaat agaagaaaaa ggtatataaa
     2041 actggcactt cttactgacc acgcaaacac ctttcgtaca gcatgatgag agcggcaaaa
     2101 gcaaactaca cttatgcttc attcgatgca catttgcaga aggttgcagc tatgaagcca
     2161 acgatgctac tcatgattac cgtattcctg actttcccag ccatttctca ggccgaatcg
     2221 ccattcagtt ctctacagtc ggcaaaagaa aagaccacgg tattgcaaga tttacgtaaa
     2281 atttgcacgc cacaggcgtc attatcagac gaagcgtggg aaaaattaat gttgtctgat
     2341 gagaataaca aacaacatat ccgcgaagct attgtggcga tggagcgtaa taatcagaat
     2401 aactactggg aagcgctggg taaagtggaa tgcccggata tgtgaatttc gcacacctcc
     2461 gcgctcaggc cagcagccgc tgacgtacgc tcgcacatca gtaaatcacc tgctttttca
     2521 aattccaatc aaaattgcac ccgttcatca ggattgtgga ggaatttccc tctacattgt
     2581 tttcatttca tcacatagaa aatcttatct cattgaaaca atacttgttt ttaatgcaga
     2641 aaaggaagcc caacggatgg gcataaaaat gagcgaattt tgatatcacc accctctaaa
     2701 aaatggtgaa gtctgaacaa agaatgttcg aaaggagaga gtagagatgg aaagcgcaaa
     2761 tctggattta gaagaaaata aagaaattgc cagtaagttc gaacgtgcat taggtatggg
     2821 agcaacactt gcagagctgc atggcattac tccggatacc cttgaaggcg tttatgctta
     2881 cgcttataac ttttatgaaa aaggaagact tgacgaagct gagctctttt ttaaatttct
     2941 ctgtatttat gatttccaaa attataacta tttaaaagga tatgccgccg tctgtcagtt
     3001 gaagaaagat tatcaaaagg cattcgacat gtatcatatc tgtttaatgc tatctcccga
     3061 taacgatttc tctttgattt attatatggg ccagtgccag atgggcttaa aaaacactaa
     3121 aatggccacg gagttattta atacagtcgt aacctacagc caaaatgaaa aagttaaaga
     3181 gatggctact gcttatcttg aactcttgac agccaatacc gaagaggaag ttcaaacaga
     3241 gtgagtcggc aaaatgaaca taaacggaaa aaactcttct tttacgcatg aagagttaat
     3301 caggaaatat catgaggaag ccccggttga tttaaaaaat aaccatggcg ataaaataaa
     3361 tgagaagaat aacaataaac acattactta ctttcccgtt gatgccattt tagatgggaa
     3421 taaacatctg ccatgagtta aataaaaaag aattaattac acccgatgat agcttaaaaa
     3481 ggaggtgaat tatgtcggca ccgattacgg gacaaacgat cacgtttgaa caaatcagcg
     3541 aaacattgcg cacacagtat agcgatgcgg aaaaaagact gcaggacagc aacaaaacgc
     3601 aagttgatcc catgcggctt aataaaaacc cgaagtccct tgataacgat attcgcactc
     3661 gtcttgaaaa caaacctatg cttgctccgc ctgaaatgca ggtttcagat tccgacaacg
     3721 ccactacagc aaagacgaac gatgcccgcc tgacaatgat tttgggtaat ctaacaggca
     3781 ttgccgatca ggatattaca acacgtttgc ataataacct tgacagcacc ctgcttcgac
     3841 acgaaatggc acataataag tttcgtgaat taagtgacgc ttactcatcg tcattagatt
     3901 ccgcgcaaaa agccgacgac atcatgcatc aggccaataa taattacaat gcggtcgata
     3961 aaaaggtgca atcgctggag aaaaaagtca acacattgaa tcaagagctc tcgcaactgc
     4021 aacctggcga tccgcaatat aataaagtag tgacgcaaaa aagtgcggct gaaaaaacac
     4081 tcacgctgtc attacaaaaa aaatcgttag ctgagaaatc gttaaataca gccattatgg
     4141 atgctgatgc tgcgatcggc caaagtatgg aaatttttga cgaaattcaa cagcaagaac
     4201 agattaataa cttcaccacc aatatttgcc tgacccagga aaaccagaaa aacagaaacg
     4261 caacagccac atttatcctt ttgattacct cagtaatgga agttattggt gataccaatt
     4321 gcgaatcgat caaaaatcaa tctgaggtaa tgaaagagat taaccatgtc agggaaaata
     4381 aactcaatga aacagcgcgt agctatacta catggacaaa agttttaaac attattaatg
     4441 aggtcgttac tgtagttaca tttgccgtaa gtgcagcctt aattgtgatt ggtattgtcg
     4501 cagcagttcc aagtgctggg gcaagctttg ctggagcgct ggcactaatt ggaggaatcg
     4561 caggggcagt agtattaggt gttgatatta tttgtcaaat cactctgggt accacggcta
     4621 ccggttggat tttgggaaaa ctaactgaag gtcttgcggg tattatttct aaatatgacc
     4681 ccacactact cgcaatctgc gctcttctgg acgccttggg tgtccaacaa acgactattg
     4741 atttagtaca agatatttac tcatctgcag ccgcttctat tgttatggta acattaatga
     4801 ttggtgcaac attaatttgt tcagtagcaa taagtgaact tgtaaaggca ctctcaaaaa
     4861 ctgccgccga agaaatcaca aaagaaataa ctgacagtat aaaatccacc gtggaatcta
     4921 tgatcaagtc agtatcaaaa aaaatcatgt cagcattaaa ttttctcggt gacaaaataa
     4981 aagcatcatc acaatttata atgatatttg caaaaataac tattcttctt gaaaaaattg
     5041 cctcgggtgt ttgtacaata attacatcga gcatgaatca atacgctgcc agtaagtctg
     5101 cagatatcga aattctacaa caagatataa gcaatctatc aaaaacgcgc gaacaaatgc
     5161 ttacagtgat gcaaaggata gataaaaccg tcgaacaaga ggtagctcag atggtgcgtg
     5221 tattacaaca ccgaactgaa gctttgaaat ttgcttctca ttctatcgta taagaggatt
     5281 caaaaatgac caataatatt agcaatataa acaatacagc tcataataag aatctcgata
     5341 aaacaacatt cgatgaacaa cctgcttatg aaacaagagt atcacaagct aaaaaaatac
     5401 tgcaaagttt tgcgccaaat gatattaaag ttgatacaaa tactaaattt attagtctga
     5461 tagagtctgt ccgtgctctc tctcctgaat tacaatgtaa ggtactagat ggtcttaatt
     5521 atgctaagaa tacactacat aatataaatc aaaattctgc catagcaaca aaatcatcct
     5581 gtcagtctac cgagtcaata gagaaaacca taacatttaa tattctcgat atgttagata
     5641 atttgacgat gagacaacag aacattatgg aggcctttct aagagtcttt acatcaatgt
     5701 acaatgcctc tgccaaggct gatgctagca tgagcatcgt ttttgaacag cttgtccatc
     5761 ttcagagtag cgaaacaaaa aatgcttctt gggcaaattt tgcaggtgca ttagcaggct
     5821 cagctattaa tgctacggga gctgtattta tggcaggtgc agaacttgcg aagcccgcgg
     5881 gaaagataga tgatatcgca gaaaaccagg aacaaaatac agacaaacca gatagccaaa
     5941 ctcggataaa atcaaaagaa gatatggcaa atgcgaaagc cagacttgcc gaaatagaaa
     6001 atcaacaaaa agatttatca aataacaaga atattgagga taatgacaaa caagagcaat
     6061 caagtaagtt acaaaaagaa aaagaaagta tcactaataa aattaaagac aaagaaaaaa
     6121 ttaatagtca caatctaaga tatggtatta ccagttctat ttctcatacc aataatctat
     6181 ttcagtctat tgcaaatcta tctcaatacg gcggaacagt ggcagcagaa catactcgtg
     6241 cagaaaaaga cttggctgag caagctaaaa atgttatgca ttcagttatg agtagctatc
     6301 tggatcaaat taatcgtgat gttcaaggaa gagatcaagc actcaattta ttacagcaaa
     6361 taatacaaac cattattgat actgatagtg caatcgttca aggcattcgc tcataaatta
     6421 ttgcaataat agttagttat atttaaggag gaatggcaca tgattaataa agataataat
     6481 ctaaatatag atattttacg tcagcagaat gcaaacattc ctcttaaaga ggcatcctca
     6541 caaagtgagg agcctcatca actggaaaac agcaaaagcg agggtaattt gtataacagc
     6601 gccagtaagg ataataatat tggcgctatg ctcaagaaag atgttgaaaa actatcaact
     6661 ttagtcagtg aacgtagccc gcgaaatgaa gactggaaat gccagcatga catacaactc
     6721 aacttcgtta aaaacatgct cagaaatttc ccaactgaga acctgtcaga gaagggatct
     6781 gcgctcttac acgaaagtta ttcgctgata aaaaacgttc tgcagcttac agaaaatgtt
     6841 aatactattg acggaaaatt aaaggatccc gctaaaatat tgaccttgca taactctgtg
     6901 agaaaaaccc tggctaacgt tgctgacaac acaaccgggt caagtgcaat ggcatcgacc
     6961 aaaggtacct taaatcacgc tatcgcttct ttagatagtt ctgcacctga agtgagaagt
     7021 tcagcaacca gcagtacttt aacggccagt agttcaaacg aatttaaaag tgataaatat
     7081 atatgcagtg atattgctga tttgatgagt gtgctcggta gcgactacct cgaaatctat
     7141 gcaaatagcg ttgagataat gagcgcatac tggcaggact tcagtgaaca tatccaaagc
     7201 aatatgggaa aatggactca ctctaataaa aaaggcgatg cgatagtttt tgacgtcaat
     7261 gcattccaaa aagccttgat gcatttttat tatatcgata aatatcctaa tggtgatttc
     7321 cactatcatt acaatccaga ctatgtttta taccctccgg cacctgctga caaaattggt
     7381 gtaccactag aagaagcaga aaaatggtgc gccgctttag gacttccggt aattccaccc
     7441 gatcccaaac accgcacacc ttctccgatt gtcgaagttg aaccgcaagg tagcggtctg
     7501 tatgttatta ttcccaaccc tcagattata gacagtatga gtcagtcttc agactcaatg
     7561 gtgcataggg acgataaagg gaaggaaaaa aatatatcta aagaattcac cggctacgaa
     7621 ataagcacgg ctgaatacca ggcatggctg gcgggttata acagccaggc agaaaatatg
     7681 aaaaccgacg tgcaggttat cacgactaaa tatagtacag ccaactccac ctacgataca
     7741 attatcaaat tattatcttc aaccattact gcgttgtttg attcagcaaa agattattta
     7801 cgtttctgat taagtaacac ccacaattat cgcccactat ctggtcccga aaaatgggac
     7861 cagcgtttca tattattaag catcagcttt cattcatttc ttcatcacaa tcttacaaaa
     7921 tgagcgtttt ttgatagagc aattctgcca atactttatt ttatattgat caaattaata
     7981 ctatgaatag agaaaacgga aggagcgttc tatgcaattg caacatggaa aaaataatac
     8041 acaacaatat atatttggcg actttgtatt aaaaaacaat ggaatattat tattcaaaaa
     8101 caaagaatat catattcccc cgaaagaact tggcgttatt attctattgc ttaatgctga
     8161 cggtgaaatt gttagcaaag aagagattat cgataaggtc tggagcgcga gtgttgccag
     8221 tgatgaatct ctaacacgat gtatttatgc attgcgcaag cttttgcatg agaataaaca
     8281 atgtaagtat atcgaaacgg tctatggtcg tggataccgt tttactgtac ctattgtgat
     8341 tgtgaccgat aatgaaccgg taaagtcgac cactactctt gccgtttttc cctttcgtac
     8401 tgaaggttcg atcaatgttc taaaactcca ttacgaactt gtccagggcc tttcaaaata
     8461 tgctttttgt ggtttagata ttttaccagc ttcggtaaca aatgaggcca gtgatttttc
     8521 cactatccat cagtttatca ataaaacagg tccagaatat tatattatgg gtcaggtcgt
     8581 tcactatggt aaaaactggc atttatttat tgagttaatt catgccagaa cacataaatt
     8641 gattgatcat caaagtattg atttcaaccc cgataatcct ctttccgtat tattatcaca
     8701 agtaattaat attcttattg aaaaaatacc caatattaat tttaaatcta ttaatacaca
     8761 acaaatgcca tctctggatt ctgctgttat gtatatgaat ggcagaatgg aaatgtatcg
     8821 ttatacacca gagagtttga aacgagcatt agtaatattt agagattgtg tgagtacgca
     8881 accgcaaaat accttgccct actgttgcct ggcggaatgc catatatcat tagccctcct
     8941 tggactcagt gaacaaaaac aagcaataac tacggcagta acctccattg aaaaggctct
     9001 tgaaattaac ccttcaaatt ctcaggcatt gggtttactg ggtttaatca gtgggctaaa
     9061 agatgaacat tcagtgtcta atgtgctatt taagcaagca catttactca aacctaattc
     9121 gccagacgtt tattattatc agtctctgct atgttttttg aacggtgacc tagcaagagc
     9181 atttaattta atagaaaaaa gcattgctct tgaacccaat aaaatgggca taagcattct
     9241 taaattaatt atactttatt acacatcccc cctcgataac gcgatatctt ttgcattaaa
     9301 tcttaacagt caaaacacct gtaataatcc aattattacc tctattctgg ctatgttcat
     9361 ggcactgaaa ggacataatg acaaagccaa aagtttattg cttaaacttg agcctgaaca
     9421 tggtcttgat tacacctgtg ttaactcgct ttatacgaag tttctgatct atggcgcatc
     9481 tataaaaaat gacattatga agttactggc aaatattaac actaacaaaa tcaatggtgt
     9541 gattttgccg ttaatttaca ctgtttatgg taagaaagaa tatgaaaagc gctggcaaca
     9601 attaattaaa gagaatgatt tatggtcaaa tgttttgctt catgaccccc gtttgctcag
     9661 tgtgaaaaat gaatttaata ctattggtat gatgcgtact tctgcgtaat aacgaatata
     9721 aattgccctg ctgatgcagg gcaatttagt gattacttcg ttaataatac tctacgcaca
     9781 agccagagga ctttcctgtt ccaagctctg atataccagt ttcaggatta aatttctcgt
     9841 gtactcccat gagctgagtg cttgtccaat aatccgtact gctaaaagcg ttcccataag
     9901 tttttaaatt accatattcg ccccacaacg tacctacttt tcgcggtact accccaccgc
     9961 ttggccgctg cgctaattct aaagatgacg gcatttgacc atagctacat gaagtattta
    10021 tgatatccca ggtattacta ctggtagcac ctaaactagt aaaccatttt ttaatcttga
    10081 atttaaatac aacatcattc cctttaccat tcttgggttt cccagtaata gtaacttcac
    10141 ttcttatcag cacagataat gttacttctc cattactgtc aacattaatt ccataaatcc
    10201 cagccgtcca attataatcc gtatttttca tattatgggt tagtaacagc gtgaattttg
    10261 caccatcaaa ccctgtttct ggaaaaccat ctgtcgccct gaattttaca ccattaacac
    10321 tcagtaactc gacttccaca aaaggaagga tggtatagga tggttgtccg tatatacgcc
    10381 aacctttaat ctccataaat gaagttaaat tttctccttc ttggtaggcc atatatgtac
    10441 gttcatatat tccatcaccc acatatctcc atttttcccc ttcttttttt tctacatttt
    10501 gtaagtacac tgcatcatcc agcaaatcca gaccgttatc caccggattt ccaaaatcat
    10561 caactaataa agcactgaca ggtaaatccg ccccttccac tactaaaact tctcgtatac
    10621 gaagctgtga accaagcgga ctcacttcac ctggtttaat agtgtaattg tttgaataat
    10681 tggcatcgct ccaaccagcc atcttcagtt gcgcattcaa attcgcttcc gcaaaatgcg
    10741 ccatatactg tctttgatat tgcccattgc cgttgtaaac ccaattattg ccctggatag
    10801 gatccgcatt cctgacttgt acgttttcct cattaagctg aacaacacca tcagtaataa
    10861 agttaccctg ggcatcttta agaacaacgg tcaccgttat gctatcccca gcgatatata
    10921 tcgttttgtc cgcgacaatc gttgattgag tttttgccgc cgcacctggt tgtattccgt
    10981 ataacgagga cttaatctcc gttgcccatg tttttaattt cagactggca actagatttt
    11041 cctcggcaac ccatgctgtc cagacaatgg tatattcacc attattctgc tcgacccacc
    11101 gcatagcatc gtgagttgcg ccgcctaccg cgaaactatc aatataagac tcgatgactt
    11161 ctgatgtcag ccctaaagca gggttaccaa actcatcccg caacgttatt gtgacggtta
    11221 atggctcccc ggcgatatat gccgatttat ctgtcctgat agcagaattt gcctgcaccg
    11281 gagcaccgct gtggatggaa taactctctg attgtttact gctgccccat tcagacaact
    11341 tcagcgtcgc gtggtgagag tctcccgcca tttgcgccga ccatgtagca gtataaacac
    11401 cggcggtttc agaccatcct cctgaacgta cggctccttc tactgttacg ttatcaccag
    11461 acaataagga ctcgccccct tcaaccaggt taccgtgcgc gtctttcagc gtcacggcga
    11521 cggttatagt gtcaccagcg gtgtaggcgt ttttatccgt agcgatagtt gatgtggctt
    11581 gcgccggaga accggactgg atgtcatagc gatctgacgt tacgccatca ctccacgtct
    11641 gcaattgcag gttggcgtgc tgattggtac tggcaatcgt cgccattcgg gttgtggtat
    11701 acacgccacc tgattcaacc cagcttccca tttctgacgg gtcagtgcca gccaccgtca
    11761 gcacaccatc agcggctaac tgtgaagctg caccggttac cagattatcg aacgcatccc
    11821 ggagcgttac cgtgatggtg attgtctctc caaccgtata gttcgactta tttgtttcaa
    11881 cgattgaagt gagttgcgat gcatcaccag gtacaaaggt gacgttctgg ctctggctgc
    11941 tgttgttgat gctggcggtg actgtactgg tgcccgccgt ctggctggtg acactgatct
    12001 ccaccgtgcc atccggcttt gtggtcatcg tcggagcaac cgttgcgctg ttgcccgcca
    12061 acacgctgac cgtctgcccg gcaagcgcat taccgaacgc atcggtgact ctcgcccgca
    12121 gcatattcgc cattgcaccg tctgccactg agtcgtcctt aatgaccacc aggtccgcga
    12181 tcttcgctgt cctgacatcg gcgataaacg tcacgttccg gctctgactg ctgctgttga
    12241 tgctggcagt gactgcactg attcccgccg tctggctggt tacagatatc tccaccgtgc
    12301 cgtccggctt ggtggtcacc gtcggagtga ccgttgcgct gttgcctgcc aacacgctga
    12361 ccgtctgtcc ggcaagcgta ttaccgttag catcggtgac cttcacctgc agcgtattcg
    12421 ccgtcgaacc gtctgcctct gaaccatcct taatgaccac cagatcagcg atcttcgccg
    12481 tgctgacatc ggcgataaat gtcacgttcc ggctcagact gctgttgttg atgctggctg
    12541 tgaccgcact gat
//
LOCUS       DAPOZO010000123        12434 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPOZO010000123 DAPOZO010000000
VERSION     DAPOZO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.123"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..417
                     /locus_tag="ROI21_004081"
     CDS             <1..417
                     /locus_tag="ROI21_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspK"
                     /protein_id="HDX5112946.1"
                     /translation="RVVQGMDAGLYQKLKPLVCALPMARQQININTLDVTQSVILEAL
                     FDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQLKTVLSVDSNYFWL
                     RSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            414..1591
                     /gene="gspL"
                     /locus_tag="ROI21_004082"
                     /pseudo
     CDS             414..1591
                     /gene="gspL"
                     /locus_tag="ROI21_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
     gene            1593..2129
                     /gene="yghD"
                     /locus_tag="ROI21_004083"
     CDS             1593..2129
                     /gene="yghD"
                     /locus_tag="ROI21_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDX5112947.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSAGYYVLIWQPLSERIEQQETMLQQLVAMNARLKSAAPNIIAARKSGTTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLNWLKALDEKYALRVAQIDVS
                     AGEKPGMVNVQRLEFGRG"
     gene            3303..4079
                     /locus_tag="ROI21_004084"
     CDS             3303..4079
                     /locus_tag="ROI21_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5112948.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLL
                     ILLGIFGYIMHRTMPDISFPVFLLNGLIPFFIFSSISKRSVSAIEANQGLFNYRPVKP
                     IDTIIARALLETLIYVSVYILLMLIVRMAGEYFEITNFLQLVATWSLLIILSCSVGLI
                     FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLITLFIGLALYRTREEAMLTS"
     gene            4076..4747
                     /locus_tag="ROI21_004085"
     CDS             4076..4747
                     /locus_tag="ROI21_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5112949.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKDLNLEIPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDSGKIITNKTVSWPVGLAGGFQGSLTGRENVKFVARLYAKQEELK
                     EKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAQLFKERHKESSFLMVSHSLNSLKEFCDVALFIPRDHQTCLYQNINDAIKLYQND
                     ENLNI"
     gene            4759..5154
                     /locus_tag="ROI21_004086"
     CDS             4759..5154
                     /locus_tag="ROI21_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDX5112950.1"
                     /translation="MRKVITFGTFDVLHIGHINILKRAKKMGDYLIVGVSSDYLNFSK
                     KQRYPVYPETERLEIIRSLKFVDEVFIEESLELKGEYIKKFKADILVMGDDWLGRFDE
                     YKELCEVSYLPRTVGISTTELISQIKRYG"
     gene            5170..6405
                     /locus_tag="ROI21_004087"
     CDS             5170..6405
                     /locus_tag="ROI21_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112951.1"
                     /translation="MKFHSEIKNSIKNAKKFKGILFVNIISYLLLTIAIRKGHSKEIL
                     KFKWLILLFGGSVAKYFVARASFLNGDYIFAKKLYQEIIKVNPIHTDSYYGLADVYRL
                     TGNKLEAKDVLIQVFNFSDRLKTWLLLANLVDTKKDYDELLNHWERECSYRNILKYHY
                     DVNDYLIIAALRAKLYEEALNRVKVLLGSINSEAFILPKTSNKNFSVNAAKIALLGLK
                     KILDANKIQFFLVSGTLLGCIRDKKLLKHDKDIDIGVWSETNLSVLSTKIACSGLFDI
                     APMRSPYTLRIKHVNGVAIDIFFHYRDHDSYWHAGSKLRWNNTPFNLISYGFLGNVFL
                     IPENYDLYLTENYGNWMQEKMKFDSAFDTPNHEIVNMYELKIHAYKKLIINMKIGSVE
                     GVRYYEDKLNELGCIISKP"
     gene            6459..10259
                     /locus_tag="ROI21_004088"
     CDS             6459..10259
                     /locus_tag="ROI21_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDX5112952.1"
                     /translation="MLLTKLAKKICQSKEYINGISLYKKKEWEKALLFFEKSIIKKTK
                     HAESYFKAGICNLKLHRYEEAFKYISKALELEPSNIQWKEQLEQCARHLDKLNNHMVS
                     KSSTEEDLLREKLNTDFNNPKLHDRLAEVLHKKGRWWQEVDTLKNAIKLDPSIPERYF
                     KLGTALEKMGRFNEASIYYKKGLEFNKKHDAIWYYALGYSLESDETNNVENVRSSKIA
                     YATAISLDKKLNSNKFGIGAFHQYKGRWAKAIDAYEKHTSINPLCAELYYRLGLSYDR
                     CYQWDKAAENYRKALSLDENHPYWHYRLGFVLERSQKYLDAAVAYQFAAQSNIKHISI
                     WYYRSGYAYAKANKYRQSCEMYIKAYDSSQEIYASQIKVIDNNDTHLNSYRKKLKYES
                     RINTLESAISNSCINYGLWYELGKTYESSCNWNDAVIAYHEGIMRNNTYTKDYYYRIG
                     YSLFRDGNYQQACNFFRKIEIFGTPYGAPDDILTKNLTFRRNATYTEFYETLSIEKNT
                     ILYESFHGASISCNPYALFLDIIDDQRFDNFRHIWVINNEKKIPEQLKNKKNVYFVSR
                     QSDLYMQCLASCEFLINNVSFPEYFIRKKGQRYLNTWHGTPIKFLGKDIKDEFLAHKN
                     VARNFLHTTHLLSPNTHTTNILLDRYDISNIFSGEIKELGYPRIDRTINLSSERKEYI
                     RRKINANVYDKVVLYAPTWRGIHGKATLDIEKLKNDLEKLADQDCHIVFRGHHMIEKL
                     VSEQNISGITIVPSEIDTNELLGAIDILITDYSSIAFDFFVMNRPVIYYAYDIEQYNN
                     ERGLYFPLNELPGTVCFNDVELLNTLSGYLRNEIYFDASKGIDKFCKNDDGSVCGKVI
                     EWFFFEEKSILLNKNKNKNILFYIGPFIPNGILSSWLNLISVIDRDKYNISLVVDPKS
                     IHGFQERFEQFKRVSPDIQVIGTCGNMLYNIEEKWLNDKLNNQFTLASKEMYDILDHA
                     YQREFLRLFGYSHIDHLIHFEGYNQSWVIRFANAPKDTVRNKIIFQHNDKLSEWRERF
                     PYLRVVFDFYKSYNKIVSVSEKTMELNRDNLSEFFNIEHDKFIYCDNVQNPDEVIKKS
                     DDIDTSGFIFENDKIYFITLGRLSVEKDQQKLINAFCRLQKLYPNIELLILGDGPLKI
                     DLQRQIITLGLEKSVHLLGRISNPFPLLKRADCFVLSSNHEGQPMVLFEAMILDKPII
                     STDITGSRSALEGRSGVLVENSVDGLFNGMRDFILGRLEFKHFDIESYQKNALSMFYE
                     KCLH"
     gene            10362..11390
                     /locus_tag="ROI21_004089"
     CDS             10362..11390
                     /locus_tag="ROI21_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XcbB/CpsF family capsular polysaccharide
                     biosynthesis protein"
                     /protein_id="HDX5112953.1"
                     /translation="MNKTVLHINSNITTDEISLLLQASKYKYIHFEIIGKGDSCKNLI
                     EFSYHDYNLKKQLIALTNAGFMSYVHRGNVTSLVHFDEIKNLWIPVKEKKFSINSDGI
                     VYTLQRAACKINEKLLIVFSQMPIEPYSASLYRYFAKNFSTIDKYIGKNVSILRVADI
                     GGITGSFYLNTNALPTNADKIKSLILEVIEQCQIKSDDVVLYGCSKGGTAAVYHGLTN
                     NYKIVAVDPILNDEHYINNKNDLHLIEGVFPQPKEELFKKVIDDYLINYKGNMSYFIV
                     SQNSEQYKYIMDIINPIITYSTVANSINDKIKSHPDVGPQSIHMIVAMINMALSGIKL
                     SNGIYDFV"
     gene            11396..12391
                     /locus_tag="ROI21_004090"
     CDS             11396..12391
                     /locus_tag="ROI21_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112954.1"
                     /translation="MALRLNSVFILAVICSIQICSAAILSKSYSHNDIHITLPLLFNE
                     YDDVLSQYDGAKYLYPSSFALDDDNNIYIQYIFEPKQKDNILVCYNKKGNYLGYYKIP
                     SGGRGGAGEGLAIIQDYNGFNVVYVVSNTGTLQRYNMVSSLYGDRLFKERDFSINAFN
                     QFSMSQDYWLIEGYESERSIKYSRNRLELYSVSGDLLRVIYLQNNYSGYIVSDASPDA
                     KNYNKRQGIALGDGFFVASYGAFYYSKMESTYNTYQGIRVFDFSGKLLSEKIFHPNEM
                     MNVLKKMGLTVSRIENEGVIVKNNTIYSMYIYSDRYHHKTSRSEGIIILLDEFTQ"
BASE COUNT         4061 a         1973 c         2577 g         3823 t
ORIGIN      
        1 cgcgtggtac agggaatgga cgccgggctt tatcaaaaac tgaaaccgtt ggtctgtgcg
       61 ctgccgatgg cccgccagca aatcaacatc aatacattag atgtcacgca aagtgtgatt
      121 cttgaggcgc tgtttgaccc gtggttaagc cctgttcagg cgcgggcgtt attacaacaa
      181 cgtccggcga agggctggga agatgtcgat cagtttcttg cacagccgct acttgcagac
      241 gtcgatgagc gtactaaaaa acagctaaaa accgtcctga gcgtggacag caattacttc
      301 tggctgcgtt cagatatcac cgtgaatgag attgaactga cgatgaattc gttaattgtc
      361 cgcatgggcc cacaacactt ttctgttctc tggcatcaga caggagaaag tgagtgagtt
      421 tcatgcttga gatttttttc ccgctttgcg ccgctgatcc catccgttgg cagcgccata
      481 cacccgacgt ggagcacggt atctggcctg acgttgctga tgaatgtctc cagcaatggc
      541 tgcaaactga tgcgattcga ctctacattc ccggcgaatg gatcagcgtc tggcaggttg
      601 aactgcctga tgtgccccgc aagcagatac cgacgattct gcccgccttg ctggaagaag
      661 agctgaacca ggatatcgat gaactgcatt ttgccccgtt gaaaatcgac cagcaactgg
      721 caaccgtagc tgtgattcac cagcagcata tgcgcaacat tgcgcagtgg ttgcaggcaa
      781 acggcatcac ccgcgctacc gtcgcgccgg actggatgtc cattccttgt gggtttatgg
      841 ctggcgatgc gcaacgggtt atctgccgca tcgatgaatg ccggggatgg agcgccgggc
      901 gggcgctggc tccggtcatg ttccgcgcac agctcaatga gcagaattta ccgatttcac
      961 taaccgtggt cggcattgca ccggaagaac tgtctgcatg ggctggtgcg gacgcgaacg
     1021 cctgaccgtt acggctctgc ccgccattac cacttatggc gaaccggaag ggaacctgct
     1081 aacagggccg tggcagcctc gtgtcagcta ccgaaaacag tgggcgcgct ggcgggtgat
     1141 gattctgccg atattgctga ttctggttgc gctggtagtg gaacggggcg tgacgttatg
     1201 gagcgtcagc gaacaggtgg cgcaaagccg cacccaggcg gagaaacagt tcttaacgct
     1261 attcctggag cagaagcgga ttgtgaattt acgctctcag atgacgatgg cgctgaaaaa
     1321 atatcgccca caggccgacg atacctggct gctcgcagaa ttgtcagtga tcgccagtac
     1381 cctgaaatca gcgtcacttt ccgacatcga aatgcgtggt ttcacctttg atcaaaaacg
     1441 ccagacgctt caccttcagc tacgggctgc gaactttgcc agcttcgaca aactgcgtag
     1501 cgcactggcg gcagattatg ttgtgcaaca ggacgcgtta cagaaagagg gtgatgcggt
     1561 ttccggcggc gtaacgttgc ggaggaaata acatgttacg cgataaattt attcactatt
     1621 ttcagcaatg gcgtgaacgc cagttaagcc gtggcgaaca ctggctgaca caacacctgg
     1681 cggggcgttc tccgcgtgaa aaaggcatgt tactggcagc ggtagtgttc ctgtttagcg
     1741 ccggatatta cgtcctcatc tggcagccgt tgagcgaacg gatagaacaa caggagacga
     1801 tgttgcagca gctggtggcg atgaacgcgc gactgaagag cgccgcgccg aatattattg
     1861 cggcgcgaaa atccggcacg acaacgccag cacaggtatc gcgggtcatc agcgacagcg
     1921 cttccgcgca ctctgtggtc atcaaacgga ttgccgagcg tggggagaat attcaggtct
     1981 ggatagaacc tgtggtgttt aatgacctgc tgaactggtt aaaggcgctg gatgaaaagt
     2041 acgcgctgcg ggtggcacaa attgatgtca gtgcgggtga gaagcctggg atggtgaatg
     2101 tgcagcgact ggagtttggg cgggggtagg tattgccatt tccttaaccc cacctgataa
     2161 cccttagccc aatggtgagt gcggaactgt caaacccgct caccgggctg agtttcgatg
     2221 tagcaagaat tatctccctc cagaccacca cggaatattc ggtaacactg aaggaacctg
     2281 tttttcacca tgtagccttc gttaatcgac acctttctca gactgtaaag ttgcttaagt
     2341 gttttgacac attatgatta ttaatagttg taataaatca ttgagtaaca attgataggc
     2401 caaaacatat aggataattc tagtgtgatc tgtattttgt gtaacctgga agttagtaaa
     2461 attcctggag ataatcagaa agggaatttc aaataagcat aataatttcc agtgaaacta
     2521 ttcgtcgatt ttaagaaatg tctgctcagg tatatctact gagggatggt gttggttgta
     2581 acactggtta aaataaacac acaaaaataa tcaagatgta tattttaatc gacaaaaata
     2641 attactcgcg gaattattga tgggtgttaa ataacaccgt ttaaatgtga tgtaaatcac
     2701 aaatgtgact gtaaagaggg tgctgtagat ataaataaga agtacatgag tgaattttaa
     2761 atagggaaat agtttcttgc taaacaattt attggtaatc aatcgcatgc gttctggttt
     2821 gagatagatt acaggtgtta ctacgcataa aattcatggg tggtattata atcaagtagt
     2881 taacagtaat agtgaaaaat aatttcttgc gaaattagcg gcccgttttg aataagtgtg
     2941 ttccggagta atccagaacg catgctgctt tacatctttt tcaagccata ttggttaagc
     3001 aaaaagaagt agaaataatt tcctgagaaa ttaactcaac attctgaaac ggccagtttt
     3061 ctgaaattac cagaagaccg aatacattca tgcctgaaga atggattaga agaaacgaga
     3121 cggaaataga tctatttatc cctgcggaaa taatttctgc tgaaattgtt tgcggctatt
     3181 aaaaaggtca aaccgtctga ctaaatttta tccagttaca aacaagtatt acctccagtg
     3241 tattggtagc tgttaagcca ggggcggtag catacctgaa gagaatagga tcacatcacc
     3301 aaatggcaag aagtggattt gaagtccaga aagtcaccgt agaggcatta tttctacgag
     3361 aaatacgaac acgctttggt aagttccgtc tgggatatct gtgggcgatt ctcgaaccct
     3421 ctgcgcattt gctgatactg ttgggcattt ttggttacat tatgcaccgc acgatgccag
     3481 acatctcgtt cccggtgttt ttacttaatg gcctgattcc cttttttatc tttagcagta
     3541 tcagcaaacg ttctgtaagt gctattgaag cgaaccaggg gctgtttaat tatcgcccag
     3601 taaaacccat cgatacgatc attgcgcgcg ccctgcttga gacgctgatt tacgtttctg
     3661 tttatattct gcttatgctc attgtcagga tggcaggcga atatttcgag ataacaaatt
     3721 ttttacaact tgtggctacc tggagtctgc tgatcattct ttcatgcagc gtcggcttaa
     3781 tattcatggt cgttggtaaa accttccccg aaatgcaaaa ggttctgccg atacttctta
     3841 agccccttta tttcatctcc tgcatcatgt tccctctgca ctcaattcca aagcaatact
     3901 ggtcatatct actctggaac ccactagtgc atgtcgtgga gttaagccgt gaggccgtta
     3961 tgcctggcta tattagcgaa ggtgtgagtc tgaactacct ggcaatgttc acattgataa
     4021 ctctgttcat tggtctggcg ttatatcgaa ctcgtgagga ggcaatgctg acatcatgat
     4081 taagattgag aatttgacga agtcatatcg cacgccaacg ggtcgacatt atgtgtttaa
     4141 ggatttaaac ctcgaaatcc cttcaggaaa aagtgttgcc tttattggtc gtaatggtgc
     4201 gggtaaatca acgttactga gaatgattgg tggcattgac cgccccgaca gtggaaagat
     4261 catcactaat aaaacagtat catggccagt cggccttgca ggtggatttc agggaagttt
     4321 aaccggacgc gaaaacgtaa aatttgttgc gcggttatac gcgaagcaag aagaactgaa
     4381 agagaaaatt gagtttgttg aagaatttgc cgaacttggc aagtacttcg atatgccgat
     4441 taaaacttac tcatcaggta tgcgatcgcg tttaggcttt ggtttaagca tggcatttaa
     4501 gtttgattat tatatcgtcg atgaagtaac ggcggtcggt gatgccaggt ttaaagaaaa
     4561 atgcgctcaa ttgtttaaag aaaggcataa agaatctagc tttttaatgg tttcacatag
     4621 tttgaattca ttgaaagagt tttgtgatgt ggcgctattc atacctagag atcatcagac
     4681 ttgtctttat cagaatatta atgacgcaat taagttatat caaaatgatg aaaatttgaa
     4741 catttagtgg agctaaaaat gcgtaaagtt atcacctttg gtacatttga tgtattacat
     4801 ataggtcata taaatatctt aaaaagggca aaaaaaatgg gggactatct aatagttggc
     4861 gtctcatccg attatttgaa tttctcaaaa aaacagaggt atcctgttta tccggaaact
     4921 gagcgtcttg aaataattag gtccttaaaa tttgtagatg aagtttttat tgaagagtca
     4981 ttggaattaa agggcgagta tattaaaaaa tttaaggcag atatcttagt gatgggagat
     5041 gattggctag ggcgatttga tgaatataag gaattatgtg aagtatcata tctgccaaga
     5101 actgttggta tttcaacgac agaactaatt tctcaaatca aaagatatgg ttaattttgg
     5161 agagggattt tgaaatttca ttcagagata aaaaactcta taaaaaatgc gaagaaattc
     5221 aaaggtattt tatttgttaa tataatatct tatctgctgc taacaattgc gataagaaaa
     5281 ggtcattcga aagagatttt aaaatttaag tggttgattt tactttttgg cgggagtgtc
     5341 gctaaatatt ttgttgctag ggcttcattt cttaatggtg attatatatt tgctaagaaa
     5401 ttatatcaag agataattaa ggtaaaccca atacatactg atagctatta tgggttggct
     5461 gatgtttata gactaaccgg aaataaatta gaagcgaagg atgtactcat tcaagttttt
     5521 aatttttctg atcgtttgaa aacttggcta ttattggcga acttggttga tacaaaaaaa
     5581 gattatgatg aattattgaa tcattgggaa agggaatgtt cttataggaa tatacttaag
     5641 tatcattatg atgtgaacga ttaccttata attgctgcgt taagggctaa actatatgaa
     5701 gaagcattaa atcgggttaa agtattgttg ggaagtataa attctgaggc ttttatctta
     5761 ccaaaaacta gcaataaaaa cttttctgtt aatgctgcta aaattgcatt attaggtcta
     5821 aaaaaaatat tggatgctaa taaaatacag ttttttttag ttagcggaac attacttggt
     5881 tgtattcgtg ataaaaaatt actaaaacat gacaaagata ttgatatagg agtatggagc
     5941 gaaacaaatc tatccgtatt atcaacgaaa attgcttgct ctggcttgtt tgatattgca
     6001 cctatgcgct caccgtatac gttgagaata aaacatgtta atggtgtggc aatcgacata
     6061 ttttttcatt atcgagatca tgacagctat tggcatgcgg gttcaaagct gagatggaac
     6121 aatactccat ttaatttgat ttcttatggt tttcttggga acgtgttttt aataccagaa
     6181 aattatgatt tatatttgac tgagaattat ggaaactgga tgcaagaaaa aatgaaattt
     6241 gactctgcat tcgatacacc taatcatgaa atagttaata tgtatgaatt aaaaatacac
     6301 gcgtataaaa agctaataat aaacatgaaa attggtagtg ttgaaggcgt tagatattat
     6361 gaagataaat taaatgaact tgggtgtatt atatctaagc cttgaggtct atataactga
     6421 atatattaaa tattaaaaaa taatcatggg gacatagtat gttgttaact aagttagcta
     6481 aaaaaatatg ccaatccaaa gaatatatta acggaatttc tctatacaaa aaaaaagaat
     6541 gggagaaggc attattgttc tttgagaaat caatcataaa aaaaacaaaa catgcggaga
     6601 gctattttaa agcaggaata tgcaatctta aattgcatag atatgaagag gcatttaaat
     6661 atataagtaa agcattagaa ttggaaccct caaatataca gtggaaagaa caactcgaac
     6721 agtgtgcaag gcatcttgat aagttaaata atcatatggt gagcaaatca tctactgaag
     6781 aagatttatt aagggaaaaa cttaatacag actttaataa tccaaaatta cacgatagac
     6841 ttgctgaggt tcttcataaa aaagggcgtt ggtggcagga agttgatacg ctgaaaaacg
     6901 caataaaatt agatccttca atacctgaac gttatttcaa acttggcacc gcattagaaa
     6961 agatggggcg ttttaatgag gctagtatat attataaaaa aggacttgaa tttaataaaa
     7021 aacatgatgc tatttggtat tatgcactcg gatattcgct tgaatctgat gaaacaaaca
     7081 atgtagaaaa tgtccgtagt tctaaaatag catatgcgac agctatatcg ttagacaaaa
     7141 aattaaatag taataaattt ggaataggag catttcatca atataaggga cgctgggcta
     7201 aggctattga tgcgtatgaa aaacatacat ccataaatcc attatgtgca gagctatatt
     7261 ataggctcgg attatcttat gatagatgtt atcaatggga taaagccgca gagaattatc
     7321 gcaaggcatt atcactcgat gaaaatcatc cttattggca ttataggcta gggtttgttt
     7381 tagagagaag tcaaaagtat cttgatgctg ctgttgctta tcaatttgca gcacaaagca
     7441 atatcaaaca tatctcgatt tggtactata gatctgggta cgcatatgct aaggctaata
     7501 aatacaggca atcctgtgaa atgtatataa aagcatatga ctcttctcag gaaatttatg
     7561 catcacaaat aaaagttatt gataacaatg atacccattt aaactcttac agaaaaaaac
     7621 ttaaatatga aagcaggatt aatactttag agtcagcaat ttctaattca tgcataaatt
     7681 atggattatg gtatgaatta ggtaaaacat atgaatcttc atgtaattgg aatgatgctg
     7741 tcattgcata tcatgaaggt attatgagaa acaatacata taccaaagac tattattata
     7801 gaatcggata ttcattattc cgcgatggga attatcaaca ggcatgtaat ttttttcgta
     7861 aaatagagat ttttggaaca ccgtatgggg ctcctgatga tattctaaca aaaaatttaa
     7921 cttttaggag gaatgctaca tacactgaat tttatgaaac attatcaatt gaaaaaaaca
     7981 ctatacttta tgaaagcttt catggtgcaa gtatttcttg taacccatat gcattatttt
     8041 tagatattat tgatgatcaa cgatttgata atttcagaca tatttgggtt atcaataatg
     8101 aaaaaaaaat ccctgagcag ctaaaaaata aaaaaaatgt atattttgtt tcaaggcaaa
     8161 gtgatttata catgcaatgt ttggcatcat gtgaattttt gataaataat gttagttttc
     8221 ctgagtattt tataagaaag aaagggcagc gatatttaaa tacttggcat ggaactccta
     8281 taaaatttct tggaaaagat attaaagatg aatttttagc acataaaaat gtggctagaa
     8341 atttccttca cacaacacat ttacttagtc ctaatacaca cacaactaat attttattag
     8401 ataggtatga tatatctaat atatttagtg gggaaattaa agagttaggt tatcctcgca
     8461 ttgaccggac tattaatctt agctctgaac ggaaggaata tatacgtagg aaaattaatg
     8521 cgaacgttta cgataaagta gtcttgtatg ctccaacttg gagaggaata catggaaagg
     8581 caactttaga tatagaaaaa ctaaagaacg atcttgaaaa gttggctgac caggattgtc
     8641 atatcgtttt ccgtggtcat catatgatcg aaaaattagt aagtgagcag aatatttctg
     8701 gtataacaat tgttccttct gagattgaca cgaatgagtt actaggggcg attgatattc
     8761 ttatcacaga ttattcaagt attgctttcg atttttttgt aatgaataga ccagtaattt
     8821 actatgcgta tgatattgag caatataata atgagagagg gttgtatttt cctctaaatg
     8881 aattacctgg aaccgtttgt tttaatgatg tagagttact taacacgtta agtggatatc
     8941 ttcgtaatga aatatatttc gatgcatcta aaggtattga taagttttgt aaaaatgatg
     9001 atggaagtgt atgtggaaaa gtaattgaat ggttcttctt tgaagaaaaa tccatattgc
     9061 ttaataaaaa caaaaataaa aatatacttt tttatattgg tccatttatt ccaaatggta
     9121 tattgtcatc atggctaaat cttatatcgg taattgatcg tgataaatat aatatttctt
     9181 tggttgttga cccaaaatca attcatggtt ttcaagaacg gttcgagcag tttaagagag
     9241 tctctccaga tattcaagtc attggaactt gtggaaacat gctttataat attgaagaga
     9301 aatggcttaa tgataaatta aataatcaat tcaccctggc aagtaaggaa atgtatgata
     9361 ttcttgatca tgcttaccaa cgagagtttt taaggctctt tggctatagt catattgatc
     9421 atctaatcca ttttgaaggt tataatcaat cttgggttat ccggtttgct aatgctccga
     9481 aagatacagt tagaaataaa ataatatttc aacataatga taagttgagc gagtggagag
     9541 aaagatttcc atatcttaga gttgtttttg atttttataa atcttacaat aaaattgttt
     9601 ctgtttctga aaaaacgatg gagttgaaca gagataactt aagcgaattt tttaatattg
     9661 agcatgataa gtttatttat tgtgataatg ttcagaatcc agatgaggtg attaaaaaat
     9721 ctgatgatat tgatacaagt ggatttattt ttgaaaatga taaaatctac tttataactt
     9781 tgggcagact ttcagtcgaa aaagatcaac aaaagttgat taatgccttt tgcagattac
     9841 aaaaattata tcctaatatt gagttattaa ttcttggaga cggccccctc aagattgatt
     9901 tacaacgcca gattataact ttaggacttg aaaaatccgt acatcttctg ggacgcatat
     9961 caaatccttt tccactgcta aagagagctg actgttttgt cctctcttca aatcatgaag
    10021 ggcaacctat ggttcttttc gaagcaatga ttctagataa acctataata tcaactgaca
    10081 ttactggttc tagaagtgct cttgaagggc gttctggtgt attagttgaa aatagtgtag
    10141 acggtctctt caacggaatg cgggacttta tcttgggaag gttggagttt aaacattttg
    10201 acattgaatc ttatcaaaaa aatgctttgt cgatgtttta tgaaaaatgc cttcattaaa
    10261 gcattaatct atatcatgat cgtcaagaac cattgattga tttgtaagga ggtatacctc
    10321 cttacaaatt aaagtaaatt tagttaagtt aataaataat tatgaataag acagttttac
    10381 atattaacag taatataacc acagatgaaa ttagtttatt gttgcaggca tccaagtata
    10441 aatatattca ttttgaaata attgggaaag gtgattcatg taaaaattta attgagtttt
    10501 catatcatga ttataattta aaaaaacaat taattgcttt aactaatgct ggttttatgt
    10561 cgtacgttca tcgtggaaat gtaacttcat tggttcactt tgatgagata aaaaacctct
    10621 ggattccagt aaaagaaaaa aagttttcta ttaattctga tgggattgta tatacgttac
    10681 aacgggctgc atgcaaaata aatgaaaagc tactgattgt tttctctcag atgccaattg
    10741 aaccatattc tgcaagcttg tacagatatt ttgcaaaaaa cttttctaca attgataaat
    10801 atattggaaa aaatgtttct atattaagag tggctgatat cggaggaatt actggctcat
    10861 tttacttaaa tacaaatgca ttaccaacca atgctgataa aattaaaagc cttatattag
    10921 aagttattga acaatgtcaa ataaaatcag atgatgtcgt actttatgga tgctcaaaag
    10981 gaggtacggc agccgtttat catggattaa ctaataatta taaaatagta gcggtggatc
    11041 ctatattaaa tgatgaacat tatataaata ataaaaatga tctacattta atagagggag
    11101 tttttcctca gccaaaggaa gagttattca aaaaagtcat tgatgactat ttaataaact
    11161 ataaggggaa tatgtcttat tttattgttt cacaaaattc ggaacagtac aaatatatta
    11221 tggatattat aaatccaatc atcacttatt caacagttgc aaatagcatc aatgataaaa
    11281 ttaaaagcca tcctgatgtt gggccacaat caattcatat gattgtagca atgattaata
    11341 tggctctttc tggaataaaa ctatctaacg gcatctatga ttttgtttaa ataatatggc
    11401 tttaagatta aattctgttt ttatacttgc ggtgatttgc agtatccaaa tttgtagcgc
    11461 tgctatttta agtaaaagct attcacataa tgatattcat atcactctgc cattattatt
    11521 taatgagtac gatgatgtac tcagtcagta tgacggggca aaatatttat accctagttc
    11581 atttgctctt gatgatgata ataatattta tattcagtat atttttgagc caaaacaaaa
    11641 ggataatata ttagtttgtt ataataaaaa aggaaattac cttgggtact ataagatacc
    11701 aagtggtggg cgaggcgggg ctggcgaagg tttggctatt atacaagact ataatgggtt
    11761 taatgttgta tatgtcgttt cgaatacggg aaccttgcaa agatataata tggtgagtag
    11821 cttgtatggt gacagattat ttaaggaaag agatttttca attaatgctt ttaaccaatt
    11881 ttcgatgagt caagactatt ggcttattga aggttatgaa agcgaaagaa gtataaagta
    11941 tagtagaaat agacttgaac tatattcagt aagtggagat ttattaagag taatttattt
    12001 acagaataat tattctggat atattgtatc agatgcgagt cctgatgcaa agaattataa
    12061 taaaagacaa gggatagctt taggtgatgg tttttttgtt gctagttatg gtgcctttta
    12121 ctattccaaa atggaaagta cttataatac atatcaaggc attagagttt ttgatttttc
    12181 cggaaagctt ttatctgaaa aaattttcca tccaaatgaa atgatgaatg ttctcaaaaa
    12241 aatgggctta acagtttcta gaattgaaaa tgagggagtg atagtcaaga ataatactat
    12301 ttacagtatg tatatttata gtgatagata ccatcataaa acctccagaa gtgaagggat
    12361 cattattcta ctggatgaat ttactcaata agaaatcaga cgagttttca ttatataaaa
    12421 gtgatatgtt tata
//
LOCUS       DAPOZO010000124        12391 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPOZO010000124 DAPOZO010000000
VERSION     DAPOZO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.124"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..515)
                     /locus_tag="ROI21_004091"
     CDS             complement(<1..515)
                     /locus_tag="ROI21_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDX5112955.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1212..1712
                     /gene="tssB"
                     /locus_tag="ROI21_004092"
     CDS             1212..1712
                     /gene="tssB"
                     /locus_tag="ROI21_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDX5112956.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSGK"
     gene            1747..1971
                     /locus_tag="ROI21_004093"
     CDS             1747..1971
                     /locus_tag="ROI21_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112957.1"
                     /translation="MAAHKNISGKHPLSDEDILQAVYRVCFSPSGEINRDGVDALITT
                     LDRRISDLLDEIIHLCEFQQSAPYWRRVLH"
     gene            2022..3497
                     /gene="tssC"
                     /locus_tag="ROI21_004094"
     CDS             2022..3497
                     /gene="tssC"
                     /locus_tag="ROI21_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDX5112958.1"
                     /translation="MSLQEEELVSSHAGQTDQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMELNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            3504..3917
                     /gene="tssE"
                     /locus_tag="ROI21_004095"
     CDS             3504..3917
                     /gene="tssE"
                     /locus_tag="ROI21_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDX5112959.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIAQCIERYEPRISRAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            3921..5771
                     /gene="tssF"
                     /locus_tag="ROI21_004096"
     CDS             3921..5771
                     /gene="tssF"
                     /locus_tag="ROI21_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HDX5112960.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAESFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            5735..6817
                     /gene="tssG"
                     /locus_tag="ROI21_004097"
     CDS             5735..6817
                     /gene="tssG"
                     /locus_tag="ROI21_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HDX5112961.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAQGEKGTPELSLRDDPAQETLRFSADASLAFPLSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSDVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            6842..8122
                     /gene="tagH"
                     /locus_tag="ROI21_004098"
     CDS             6842..8122
                     /gene="tagH"
                     /locus_tag="ROI21_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HDX5112962.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSTDPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDFPVAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQIYDRVLREKQREPEA"
     gene            8119..8643
                     /gene="tssJ"
                     /locus_tag="ROI21_004099"
     CDS             8119..8643
                     /gene="tssJ"
                     /locus_tag="ROI21_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HDX5112963.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            8646..9977
                     /gene="tssK"
                     /locus_tag="ROI21_004100"
     CDS             8646..9977
                     /gene="tssK"
                     /locus_tag="ROI21_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HDX5112964.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9982..10743
                     /gene="icmH"
                     /locus_tag="ROI21_004101"
     CDS             9982..10743
                     /gene="icmH"
                     /locus_tag="ROI21_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HDX5112965.1"
                     /translation="MDEGSLSLPPFTGHHEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            10752..12391
                     /locus_tag="ROI21_004102"
                     /pseudo
     CDS             10752..12391
                     /locus_tag="ROI21_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT         2954 a         3317 c         3302 g         2818 t
ORIGIN      
        1 gcttccagcg gtgcgcgcca gtcgtcggca ccggaggtgc cagacttgac gtgttcccac
       61 tctattttgc ggtaggccag tgaaacttcg agcaactggg tgaactcgcg tttcgccgga
      121 tcctggcaat gcggcatatg cagtttcata tcgacaatcg tcgaatcggt caggcgggtg
      181 gtgaaataat gctcctgttt gccttccaca gaggtacgcc accagtgaag ttcagtggtt
      241 ggcagcattt cgccggaggc cagtgcgttg tacatcagcg gaacagcttt gttcagcgcc
      301 acggtaaaaa tgaacggctt gtgagcacgc tgacccgcag gctggccgga ctgaggatcg
      361 gtcgggacag taacgttatg caggaattcc tgcaccagca tctcgtcttc gtgcccctgc
      421 acatagatgt tgccgacaga atcagcggta aatgcacctg cagtaatatt tccctgggtc
      481 tgaccagtga tggagatata acacggtgtt ggcatttgta aactccttgt taacgtggtt
      541 aattactact tacaatgacc tgccgttaat cccagacagg cagatgcagc tcacttcacg
      601 gaaccgcatc atttttcagt attttttaca tacaaaccga gctagaattt acggttgtcg
      661 taaaaactat ttttaaaatc attaaatctc ttattcagca ataagttaat aatatatatc
      721 tggataacca tacccaacta ttcaaagtcc gtattgcctc cataaaatgg ccaaagctga
      781 atcatgaata aatattgtat ttatcctttc tgaattatca ttttgataat ttttgagata
      841 ttccttaaaa atacccactt aatcaatcac aaaacaaata tataaacaag caatcatcat
      901 attagcttat gtttaatgaa attattatat aattcaataa gttatataaa aactctatat
      961 ttaaattatt gccataatac acctgacgcg aatttcgcag gttcacacaa tctatttaac
     1021 gcttgcagaa tacctctctt tacgatcgat aaaaagtcct tttcttattt attttgaagc
     1081 gtagaatgcg gggtgatgaa ggagtggtga ttttattttc accgccatta gggcggcatt
     1141 gccgatattt ttttgcggta caaatatgcc gtgttcatgg cctgtacgag ataacatgac
     1201 ggatcacttt tatgagcaaa aaatttgaag gcagcgtcgc gcctcgcgag cgtataaata
     1261 tatcctatgt cccgaagacg gacggccaga cggctgaagt ggagcttccg cttaacatgc
     1321 tcgttgtcgg cgataccgga aatacgcagg agacatcccc actggatgaa cgtcaggcag
     1381 tttccgttaa caagcacaat ttcggtgcgg tgatggctga agcagccatt ggcctcaatt
     1441 ttaccgtccc cgccacgctg aagggaagca caacggacga tgaactgaat gtggcgctga
     1501 atatcaagtc actggatgat ttttcaccgg acagcgtggc ccggcaggtt ccggaagtta
     1561 ataaattgct ggaattgcgt gaagcactga ctgcgctgaa ggggccgatg ggcaaccttc
     1621 cggcattccg tacccagctc caggcattgc tggaaaatga ggagtcccgc gagcagttgc
     1681 tcaaagaaat cggccaagtg agtgggaaat aaccaccact atttcattaa acagggttat
     1741 ccagacatgg cggcacacaa aaatatctcc gggaaacacc ccctctctga cgaagacatt
     1801 ttgcaggcgg tttaccgggt atgtttcagc ccgtccggtg aaatcaaccg ggatggcgtg
     1861 gatgcgctga ttacaacact ggatcgccgt atcagtgatc tattggatga gattatccat
     1921 ctttgtgagt ttcagcagag cgcgccgtac tggcggcgcg tacttcactg acggcgaaaa
     1981 cccggtttca gaaacacagc taaaacagaa ggattttcag tatgtcttta caggaagagg
     2041 aactcgtttc cagtcatgcc gggcagactg atcaggcatc gtcgctgctt gatcaaatca
     2101 tggctcagac ccgtattcag cccggttcag aaggttatga cgttgcccgc cagggtgtga
     2161 cagcctttat tgccagcatc ctgcaatcca ctgcatctgc cgaaccggtc aataaactgg
     2221 ccgtggacag catgattgca gatattgatg aacgcatcag ccgccagatg gatgtcatca
     2281 tccatgcgcc tgcctttcag caggtcgagt cgttctggcg ctcactgaaa accatggtgg
     2341 atcgcgttga tttccgtgaa aacatcaagg tcaacgtgct tcatgtcacc aagcaggaac
     2401 ttctggaaga ctttgagttt gcgccagaaa ttattcagtc cggattttat aagcacgttt
     2461 attcgtccgg tttcggacag tttggcggtg aacccatcgc tgccgtgctg ggcgcttatg
     2521 agttcaaaaa taccgcgcct gacatgaaac tgctgcaata cgtcagtgcc gtgggcgcaa
     2581 tggcgcacgc accgttcctg tcctccgttt ccccggagtt tatggagctg aactcgtgga
     2641 ccgaactgcc taatatcaaa gatctttatg ccatctttga aggtccggcc tacaccaaat
     2701 ggcgcgccct gcgtgactcg gaagattcgc gctatttagg gcttacggca ccccgcttcc
     2761 tgcttcgcca gccctattca ccaacagata accctgttaa gaactttaac tattacgaag
     2821 atgtcagcca gaaccacgaa gattatctgt ggggtaatac ggcctggatg ctggcatgca
     2881 atatcgcaga cagttttgcc aaataccgct ggtgtccaaa cattatcggt ccgcaaagcg
     2941 gcggcgcagt gaaagatctg ccggtgcatc tgttcgaaac gatggggcag attcaggcca
     3001 aaatcccaac cgaggtgctg gtcaccgatc gccgcgaatt tgaactggct gaagagggtt
     3061 ttatcaccct taccatgcgt aaagactctg ataatgcagc ctttttctct gcaaactcgg
     3121 tacaaaaacc gaagcacttt ccgggaaaag acgcagaaac caactataag ctgggtacgc
     3181 agcttcctta tctcttcatc atcaatcggt tagcgcacta catcaaggtg ttgcagcgtg
     3241 aacaactggg gtcatggaaa gaaaggagcg acttagaaag agagcttaat acctggatcc
     3301 gtcagtatgt tgccgaccag gaaaacccgc ctgcagacgt gcgcagccgc aaacctctgc
     3361 gcgctgccaa agttgaggtt atggatgtag aaggcgaacc cggctggtac caggttgcgc
     3421 taagcgtgag gcctcatttt aaatttatgg gggcaaattt tgagctttcc ctggttggcc
     3481 ggttagacag ggagtaacac accatgatac cggatcggga aagaaccaca ggttctcttt
     3541 ttgagcgtat ggaagcctct tcagccagaa accggcaggg aggcagtatt cattcgctca
     3601 ggcaatccat tcgccagaac ctacgaaaca ttcttaatac ccggtccggt agttgccggg
     3661 gtgcgcctga gctgggcatt gatgaaccgg aaggggctga aaacttccgc gaatcaatga
     3721 gtcgggccat cgcgcaatgt atagaacgct atgaaccccg gatctcacgc gctgaagtgc
     3781 aggcagtggt ttcatccgcc tccagcccgc tggatatgac gtttcatatt acagcctggg
     3841 tgacatttaa cgaaacgcat gaggtgcttg aatttgatat ggcaccgaat ggcagccagc
     3901 attaccgggt ggactgacaa atggaatttg aagaacgcta tttccgggaa gaactcgatt
     3961 acctgcgcca gcttagcaag ctgctggcaa cggaaaaacc ccatcttgcc cgcttcctgg
     4021 ccgaaaaaga tgcagatccg gatattgaac gcctgctgga aggggtggct tttcttaccg
     4081 gcaatctccg ccagaaaatt gaggatgaat tcccagaact gacgcacggg cttattaaga
     4141 tgctatggcc taattacctg cgtccggttc cggcaatgac ccttattgaa tatacgccgg
     4201 atatggataa gtcttctgtg ccggtgttaa tcccccgtaa tgagcagttt acaaccaacg
     4261 ccggggaaat tagagtcgat gaagtgctgc cctccgatgc taaaaaggag gagccgcctc
     4321 cctgtacctt caccctctgc cgggatatct ggctgctgcc cgttcgcctg gagcagattg
     4381 aaaaccgcag tacgacccgt aatggtgtta tcaacatcac cttttcggtc gcaccgggaa
     4441 cggacttccg cacgctggat ctgaacaaac ttcgcttctg gctcggcaat gacgacaact
     4501 atacccgtga ccagctttat ttatggttct gtgaatactt gcagggtgcc gacctgactg
     4561 tgggtgaaca gcatattcgc ctgcctgagt ttatgctgaa agctgtcggt tttgagccac
     4621 aggatgccat gctgccctgg ccgaaaaacg tccacagcgg ctaccggatc cttcaggagt
     4681 atttctgtta cccggatgcg tttctctttt ttgatctttg tggttgtccg gcgttgcctg
     4741 acggattgca ggctgaatcc tttaccctgc aactgcgttt ttcgcgccca ttgcccgtgg
     4801 acatccggtt gcgccgcgac tccctgcgcc tgtattgcgc acctgccatt aatttattta
     4861 tccaccatgc agaagccatc acgctggaca accggcgggc agactatccg ctggttccca
     4921 gccgccatta tccggaacat tacgatgtct tttccgttaa cggcgtgata agccaggtgc
     4981 aggatatgtt caggaaaaaa gatctggggc gtcctgtttc gacgcaggcc gcgcgccagt
     5041 ggcctgcctt tgaaagtttc agccatcaga tggaatacag ccggaagcgg gaagtggtgt
     5101 actggcatca ccggaccaaa acatccctgt tccatcgcgg ctttgatcat acccttgctt
     5161 ttatacatgc tgatggcagt tatccgtcag acgaatcgct gctcagtaat gaagtggttt
     5221 cggtatcgct gacctgtacc aaccgtgagc ttccgtcaca aattcgttcc ggcgatatca
     5281 ccggcacaac cggtaaaaat gcggctgttg cttcatttcg caacattacc cgcccgacgc
     5341 aaccgctctg gccggtcatt gatggcagcc tgcactggtc cctgctctcc gccatgaacc
     5401 tgaattatct gtcattactg gatacggacg cgctgaagca ggtcatcgcc aactttgatc
     5461 gccacgcaat ccatcatccg cagacggcgc ggctgtcaca acaaaagctg gatgccattg
     5521 agcgtctgga gactcgccct gtcgaccgcc tgtttacggg cattcccgtc cgggggctgg
     5581 cctccacgct gtatctgcac ccggagccgt ttgtctgtga aggggaaatg tatctgctcg
     5641 gtacggtgct ttcgcatttt ctgtcgctgt acgccagcgt taactcattc cacatgctga
     5701 ccgttgtgaa cacagaaagc caggagacat ggaaatggac ggaaagaacc gggcagcatc
     5761 ctcttatctg agtccgggaa atcccccggc agataaagag cagaatgatc cgctggcaca
     5821 ggtttttcat aacgcctgct cttacaattt ttttgcgatg gcggagctgt tgcaccgcct
     5881 ggcacagggt gaaaagggga cgccggaatt atccctgcgc gacgatccgg cacaggaaac
     5941 cctgcgtttc agcgccgatg ccagccttgc gtttcccctg agtgatatca gtgcgctgaa
     6001 acgggatacg tcaggggcat tcaggatgac cactaccttt atggggttac aggggagcca
     6061 gtcgcctctc cccggttatt acctcgatca cctggcctgg aaagcggtac atgaacaaag
     6121 tccggtaggt gattttctgg atatgttcag ccaccgcctg acccagtttg tctggcatat
     6181 ctggcgtaag taccgctatc acatcagttt tcgtaacggc ggtgtggatg cgttctcgca
     6241 gcgcatgtac tcccttgttg ggctgggcca ccgccagctt cgcgataagc tggcgattaa
     6301 tcacagcaaa atgctggcgt attccgggat cctggcaaat ccgggacgat ccccggaaat
     6361 aatctgcggt ctggtgtccc actgttttga tttgtcagac gtgacgctgc aaaactggca
     6421 acgtcgcaag gtggatattg aaccggatca gcaaaatagc ctggggagtt attcacttaa
     6481 aaatggcgaa aaactggcag gaagatcggt actgggcaac tttgttctgg gtacacgggt
     6541 gcccgatctc agcggaaaat tccagctcag tattaccagc ctgacccgaa aacagttcct
     6601 ctcttttctg ccgtccggcg aaaacttcct gccactgacg atgtttgtgt ccttcatcct
     6661 gcgcgatcag cttgcctggg acttacatct ggggctggcg ccggagcagg tgggcgcaat
     6721 gcgcctgggc gataacaaaa gtgcgctgct gggctggacc agcttcctcg gcacaccgga
     6781 agaacgacct tcagtcacca tcagggtacg gtcgtaaatt atgataatca cggagcagaa
     6841 tatgccggaa gagaaactac agacgctttc attgcaggtc attaacggca gtgagctgga
     6901 aagcgggcgg gcggcgcgct gtctgttcac acagcaggga aatgtcggcc atggccccga
     6961 atgccactgg tcggtacagg atcgtcagca gagcattccg gctcaggctt ttaccgttat
     7021 cctgcacgat ggcacatttt gtctgcgccc gcagacggca caactgtggc tgaaccaggc
     7081 aaaagtcaca gcaacatcag acctgataca gctgcgccag ggcgatgaga tccagatagg
     7141 gcgtctgatg gtgagggttc atctgaaccg gggagatatc ccccattacg atgaggaaat
     7201 ggccactccc gaaaccatcg tcaccaatcg cgatatgctc acggataccc tgctgtcaac
     7261 ggagggtgcg ccacactatc cgggaatgac tcaccggcat cagcttgcag acaccgtggt
     7321 aaacggtttt tctaccgatc cactccaggc acttcagtcc gaaagcctga ttaccacggg
     7381 cgatccgctt tcaggcattg cggctgtccg gccatcggca ccgctgtccg atccggcaag
     7441 taatggtggg atcaatactc cgtttatgga tctaccgccc atttatgcca gccccggcga
     7501 tcgtaatgat gacttccctg tggcagaaat ggcgcaacgc caccttgcgg tcaccccctt
     7561 actgcgcggt ctcggcggct cgcttaccgt gagcaattct gacgatgcgg atgattttct
     7621 ggaagaggcc ggacgcacgt tacaggccgc aataaaaggt ctgctcgatt tgcagcaaca
     7681 gcgtaacagc ctctcagaca aacatttgcg cccgctggaa gataacccgc tgcgcctgaa
     7741 catggattac gccaccgcgc tcgacgtgat gtttgccgaa ggtaaaagtc cggtacatct
     7801 ggcggctccc gccgctgtca gtgaaagcct gcgtaatgtc cgccaccacg aagaagctaa
     7861 ccgggcagcg attgtggagt cacttcgtgt cctgcttgac gctttctcac cacaaaatct
     7921 gctgcgccgc tttgtgcagt accgccgcag ccatgaactg cgccagccgc tggatgatgc
     7981 cggagcatgg caaatgtaca gccattatta cgaagaactg gcctccgatc gccagcaggg
     8041 gtttgagatg ctgtttaacg aggtctacgc ccagatctat gaccgggtgc ttcgtgaaaa
     8101 acagcgggag ccggaagcat gaacaggaag gcgtttctgg cctgcgtgct gatgtgcgtc
     8161 atattaaccg gctgcgaaac ggcgaaaaaa atcagcgagg tgatcaaaaa cccggatatc
     8221 caggtcggga gcctgaagga gcaaccttcg gaaataaccg tcaccctgct gaccgaaccg
     8281 gacaccaata ccaatgcgga gggtgaatca gccgccgtgg atgtgcaact ggtgtacctg
     8341 actgacgact caaaattgct ggcagccgac tatgaccaga tagccagtac gccgctaccg
     8401 gatgtgttgg gtaaaaacta tatcgaccac caggatttca acctgttgcc ggacaccatc
     8461 aaaaccctgc cgccggtaaa gctggatgag aaaacgcagt ttattggtgt cgtggcctat
     8521 ttctccgatg accaggccac tgaatggaaa caaatcgaga cggtggaagg aaccggccac
     8581 cattaccgcc tgctggtgca tgtacgtcaa agcagcatcg aaatgaaaaa agaggatgaa
     8641 taattatggc aaccacccgc aacaaggtga tgtggcagga agggatgctg atgcgcccac
     8701 accatttcca gcaacagcag cgttacaacg actacctgga taaccagcgt ttccgggcca
     8761 tgaatgattt atcctgggga tttaccgaac tcaccctcaa caatgaactg ctggcgcagg
     8821 gtaagatcat gattgacagc gcgtcaggca cactgcccga cggcaccgtc ttttctatcc
     8881 ccgaccagga cgcactgccc gatccgctgc acccgcaaca ttttccggac gaaagaagcc
     8941 gcaatattta cctcgctctg ccggtcgcca gtgatgtgag aaatgaaatc agcgacgggc
     9001 ggcgaatcgg gcgttaccgg ctgaattatg ccgatgtccg ggatctgcat tcagaagaag
     9061 gtgacgcgcg aacactgacg ctgggtcaac tgacgccgcg cattatgagc ggtgcagaag
     9121 atatgagcgc ctatattacg ctcccgcttt gccgtatcag cgatcgccat gccgacggct
     9181 ccctgacgct ggatgacgat tttatcccct cctgccagaa tattcaggtc agtaagaaac
     9241 tgcgtgttta tctcaaagag gtacaggggg ccattggcgg acgggcaagc gatctggcaa
     9301 accgcattgg ctctccggcg cagagcggca tcgcggatgt ggcggaattt atgatgttgc
     9361 agttacttaa ccgtaaccag acccggttta cccatcgcgc tcgtcgatcc cagctccacc
     9421 cggaagattt ctaccttgat cttgccgggt tgctgggtga actgatgacc tttaccgagc
     9481 cgtcgcgcct gccctgcccg cttgatgtgt atgatcatca tgacctgacc aaaacattta
     9541 aaacgctgtt accggaagtc aaacgggcgc tgcataccgt actgtcgcca agagccgtta
     9601 atctgccgct gcatctgcgt gacggtatct ggcaggccga tatccatgac acagaactgc
     9661 tgcaatctgc cacctttgtg ctggccgtgg cggcaaacat gccggtcgat cagatccagc
     9721 gccagtttat ccagcagtcg aaaatttcct cgccggaaaa aatccgcaat atggtcagtg
     9781 tgcagatacc cggcattcca ttgcgtgccc tgatggtggc tccccgccag cttccttacc
     9841 attccgggtt cagctatttc gaactcgaca agagcggcca ggcctggaca gaaatggctg
     9901 ccgccggggc cgttgcactg catgtatccg gcagtttccc ggatctgaac atgcaactgt
     9961 gggcgataag agggtaagac gatggatgag gggtcactgt ctctgccacc atttaccgga
    10021 caccacgaaa aaagccagcg cgactatcat ctcgcgcttc gtggcaacag cctgaatccg
    10081 atgatcgacg cagccacgcc gctcctcggt atggtgatgc gcctttcaac catgaacagt
    10141 cagacgatgc cggagcatct ttttgctcag gtggtgacag atgttcaggc cgtggagcaa
    10201 ctgcttcagg agcagggcta cgaaccgggc gtgattatct cgtttcgcta catcctctgc
    10261 acgtttattg atgaagcggc gctcggtaac ggctggtcga acaagaacga gtggatcaag
    10321 cagtcattgc tggtccattt ccacaacgaa gcctggggtg gagagaaagt tttcatcctg
    10381 ctggagcgcc tgatccgtga acccaaacgt taccaggatc tgctggaatt tctctggatc
    10441 tgtttttctc tgggatttcg cgggcgctac aaagtggcag cacaggatca gggggaattt
    10501 gagcagatct accgccgtct gtaccacgtt ctgcataaac tgcgcggcga tgcccctttc
    10561 ccgctgttac accaggacaa aaaaacccag ggcgggcgtt atcagctcat cagtcgcctg
    10621 actgttaaac atattttttg tggtggcgtt gtcgtgctgg cgttgtttta cctgttctat
    10681 ctgttgcggc tggacagcca gacgcaggac atcctgcacc agctaaacaa attacttcga
    10741 taaggacgtt tatgatccag attgatcttc ccacgctggt aaaacggctg aacctgttct
    10801 cccgccaggc gctggagatg gccgcctctg aatgtatgag tcagcaggca gcggaaatta
    10861 ccgtcagcca tgtgcttatt cagatgctcg ccatgccacg cagcgacctg cgggttatta
    10921 cccgccaggg cgatattggc atggaagagt tgcgccaggc gctgacggtg gagaactaca
    10981 caactgcccg ttctgcggac agctacccgg cattttcccc gatgctggtt gagtggctga
    11041 aagagggctg gctgctggcg tcggctgaga tgcagcacag cgaattgcgt ggcggcgtgt
    11101 tgctgctggc cctgctgcat tcgccgctgc gttatatacc gcctgctgcc gcccggttgt
    11161 tgaccggcat taaccgtgac cgtctgcaac aggactttgt acagtggaca caggagtcgg
    11221 cggaatcagt cgtgccggat gcagacggta aagtcgcagg cacactgacg gacgctgctg
    11281 acaccctgct tgcccgctac gccaaaaaca tgaccgcaga cgcccgtaac ggcaggcttg
    11341 acccggtact gtgccgcgac cacgaaatcg acctgatgat cgacattctc tgccgccgcc
    11401 gcaagaacaa cccggtggtg ggcgaagcag gcgtgggtaa aagtgcctta atcgaagggc
    11461 tggcgctgcg catcgtggca ggccaggtgc cggacaaact gaaaaacacc gatatcatga
    11521 cccttgacct aggcgcattg caggccgggg cgtcggtgaa gggcgaattc gaaaaacgct
    11581 tcaaggggct gatggcggag gtcatttcct ctccggtgcc ggtcattctg tttattgacg
    11641 aagcacatac cctgattggc gcgggcaacc agcagggcgg gctggatatc tccaacctgc
    11701 tcaaaccggc gctggcgcgc ggcgaactga aaaccatcgc cgccaccacc tggagcgaat
    11761 acaaaaaata cttcgagaaa gatgccgccc tgtcgcgccg cttccagctg gtgaaggtca
    11821 gcgaacccaa cgccgccgaa gccaccatta ttctgcgtgg tctgtcggcg gtctatgaac
    11881 agtctcacgg cgtgctgatt gatgatgacg ccttgcaggc cgcagcaacg ctgagcgaac
    11941 gttatctctc cgggcgtcag ttgccggaca aagccattga tgtgctggat accgcctgcg
    12001 cccgcgtcgc cattaacctt tcctcgccgc cgaagcaaat ctcggcgctg accactctga
    12061 gccaccagca ggaggcggaa attcgccagc ttgagcgcga gcttcgcatc gggctgcgta
    12121 ccaacacctc acggatgacc gaggtgctgg tgcagtacga tgaaacgctg acggcgctgg
    12181 atgaactgga agcggcctgg catcagcagc aggcgctggt ccgggagatt atcgcgctgc
    12241 gccagtagtt gctgggcgtg gcagaagacg atgcggcgtc gttgccggac gcagatgccg
    12301 cggaggatac gccgccagag tcagagtcag agtcagagtc agagtcagag tcagagtcag
    12361 agtcagagtc agagtcagag tcagagtcag a
//
LOCUS       DAPOZO010000125        12369 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPOZO010000125 DAPOZO010000000
VERSION     DAPOZO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.125"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..1182
                     /locus_tag="ROI21_004103"
     CDS             <1..1182
                     /locus_tag="ROI21_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5112966.1"
                     /translation="ASTATYINKSGVFSVAKIDEPRFEKEGLLIEGQRTNYFVKSNTP
                     AEWTSTSNIDKTNNGVDEFGFSYAKMRTKDNMTGQSSALSLHTCSASRGIDVSGDNKY
                     CTVSCRVKAPDGLRCRLRFEKYDGSVYTFLGDAYLTFGTLIIEKTGGAANRIAATATK
                     DPVTGWIFYEATIEAVEGETLIGAMIQYAPKKGGITEAGDYIYLATPQFENGGCASSF
                     VITTTAPATRSSDMVTIPTENNIYNRPLTCLVEVNRNWGDIPPNVAPRIFDFSGVPPI
                     ESITYAFNTTEKYYGQLYMQTYKASTSTYVSSVFAGRADVRKFIGGFNIYSDGTKRVV
                     SNGEATKTMKTEWTGVKTRTFIRIGGQATSGTRHLFGHLRNLRLWHKELTDAQMGESI
                     K"
     gene            1179..1460
                     /locus_tag="ROI21_004104"
     CDS             1179..1460
                     /locus_tag="ROI21_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112967.1"
                     /translation="MKDLTLKFADRADFSAFMESIGYYDDESMQDDILIDVIGNVYKE
                     TGELTEDGEPVCVKEDGYFVNVRIINDSQISSLFDEHAVAVEHQLRSWM"
     gene            1470..2174
                     /locus_tag="ROI21_004105"
     CDS             1470..2174
                     /locus_tag="ROI21_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HDX5112968.1"
                     /translation="MATSTVIPDDIKTLKGDVSKAKEDISSINVKVSTLQTDMDSAKQ
                     DISTRYTKTEVDNKLKNKVEVNDLESGRYGGDFYPLTGREAFYLWGLGTTTAAANLYL
                     NPDPAISSVLRSTSSIRYKHSVETIDSEHADLIFRMRPVWYRSQCENDRRDWGFYGLI
                     AEEVGEIAPQFVHWRPANEDDAPETISSNGLVAEGVMYERLVVPLIHHIQKLTERVDE
                     LESELKLLSVSRSDIG"
     gene            2185..2478
                     /locus_tag="ROI21_004106"
     CDS             2185..2478
                     /locus_tag="ROI21_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112969.1"
                     /translation="MDITPFLHALCAVAAQVLVGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
     gene            2498..2608
                     /locus_tag="ROI21_004107"
                     /pseudo
     CDS             2498..2608
                     /locus_tag="ROI21_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(2706..3296)
                     /locus_tag="ROI21_004108"
     CDS             complement(2706..3296)
                     /locus_tag="ROI21_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDX5112970.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(3613..3846)
                     /gene="ydfK"
                     /locus_tag="ROI21_004109"
     CDS             complement(3613..3846)
                     /gene="ydfK"
                     /locus_tag="ROI21_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HDX5112971.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            complement(3915..4028)
                     /locus_tag="ROI21_004110"
     CDS             complement(3915..4028)
                     /locus_tag="ROI21_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112972.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            4632..5915
                     /gene="ydfJ"
                     /locus_tag="ROI21_004111"
                     /pseudo
     CDS             4632..5915
                     /gene="ydfJ"
                     /locus_tag="ROI21_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            6004..7464
                     /locus_tag="ROI21_004112"
     CDS             6004..7464
                     /locus_tag="ROI21_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDX5112973.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(7500..7703)
                     /gene="ydfZ"
                     /locus_tag="ROI21_004113"
     CDS             complement(7500..7703)
                     /gene="ydfZ"
                     /locus_tag="ROI21_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDX5112974.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(7881..8567)
                     /gene="ydfH"
                     /locus_tag="ROI21_004114"
     CDS             complement(7881..8567)
                     /gene="ydfH"
                     /locus_tag="ROI21_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDX5112975.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(8656..9402)
                     /gene="ydfG"
                     /locus_tag="ROI21_004115"
     CDS             complement(8656..9402)
                     /gene="ydfG"
                     /locus_tag="ROI21_004115"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDX5112976.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            9539..11584
                     /gene="dcp"
                     /locus_tag="ROI21_004116"
     CDS             9539..11584
                     /gene="dcp"
                     /locus_tag="ROI21_004116"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDX5112977.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQISNHHYRPAFDEGMLQKRAEIT
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEVQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLKRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            11949..12341
                     /gene="ydeI"
                     /locus_tag="ROI21_004117"
     CDS             11949..12341
                     /gene="ydeI"
                     /locus_tag="ROI21_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDX5112978.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         3270 a         2619 c         3058 g         3422 t
ORIGIN      
        1 gcatcaactg ccacatatat caataaatca ggcgtattta gtgttgctaa aattgatgag
       61 ccacgatttg aaaaagaagg tttattgatt gaaggacagc gcactaacta ttttgttaaa
      121 tccaatactc ccgctgaatg gacgagtacc agcaatatcg ataaaactaa taatggtgtt
      181 gatgaatttg gtttttcata tgccaaaatg cgaacaaaag ataatatgac aggacaatca
      241 tctgcactta gtctgcatac atgcagtgca tcccggggga ttgatgttag tggcgataat
      301 aagtattgca ctgtttcatg cagggttaaa gctcctgatg gtcttcgttg tcgtttgcgt
      361 tttgaaaaat acgatgggtc ggtttataca tttttaggag atgcttattt aactttcgga
      421 actctgataa tagaaaaaac tggcggggca gccaatagaa tagcagctac tgcaactaaa
      481 gatccggtta cagggtggat tttctatgag gcaactatag aagctgttga aggtgaaacc
      541 ttaattggcg caatgattca gtatgcgccg aaaaaaggtg gtataactga agcgggagat
      601 tatatttacc ttgcaacacc acaatttgaa aacggcggat gtgcttcatc ttttgttatt
      661 acgacaactg cacccgcaac ccgctccagt gatatggtga cgatcccaac tgaaaataat
      721 atctataata gaccgcttac gtgtcttgtc gaggttaata gaaattgggg cgatattcct
      781 cctaatgtag caccgcgtat ttttgatttt tctggtgtgc cacctattga gtcaattaca
      841 tacgctttta acacaactga gaaatattac ggtcagcttt atatgcaaac ttataaagcg
      901 tcgacaagta cttacgtttc tagtgtgttt gctggtcgag ctgatgttcg aaaattcatt
      961 ggtggtttta atatttattc tgatggtact aaacgagtag tttctaacgg tgaggctact
     1021 aaaactatga aaacggagtg gacgggcgta aaaacacgga cctttattcg aattggaggt
     1081 caagccacat cgggaactcg tcatctattc ggccatttga gaaatcttcg tctctggcat
     1141 aaagaattaa ctgatgcgca aatgggggag agtattaaat gaaagattta acactcaaat
     1201 ttgcagacag ggccgacttt tcggccttta tggagagcat tggctattat gatgacgagt
     1261 cgatgcagga tgatattctt attgacgtga taggtaatgt gtacaaagaa accggagaac
     1321 ttactgaaga tggcgagccg gtatgtgtta aggaggacgg atattttgta aatgtgcgca
     1381 tcattaatga ttcgcaaata tcgtcattat tcgatgaaca cgcggttgct gttgagcatc
     1441 aactccgtag ctggatgtga ggaagaaaaa tggctacatc gacagtaatt cctgatgaca
     1501 tcaaaacgct aaagggagat gtcagtaagg caaaggaaga tatttcctca attaacgtaa
     1561 aagtatcaac gcttcagact gatatggaca gtgcaaagca ggatatcagt accagataca
     1621 caaaaacaga agtggataat aagctgaaaa acaaagtgga agtgaacgat ctggaaagtg
     1681 gtcgttatgg cggagatttt tacccgctga ctggccgtga agcgttttat ttatggggat
     1741 tgggcacaac tacagcggcg gcaaatcttt atcttaatcc tgaccctgca atttcgtctg
     1801 tgctgcggtc aacatcgtct atccgctata aacattcagt agagacgata gattcagagc
     1861 acgccgatct cattttcagg atgcgccctg tgtggtacag gtcgcaatgc gaaaatgaca
     1921 ggcgtgactg gggattctat ggattgattg ccgaggaagt aggagaaatt gcccctcagt
     1981 ttgttcactg gcgaccagcc aacgaagatg atgcaccgga aaccatttcc agcaatggcc
     2041 ttgttgccga aggtgtaatg tacgaacgtc tggttgttcc actgattcac catatccaga
     2101 agctgactga aagagttgat gaacttgagt cagaattaaa gttgttatcc gtttcccgaa
     2161 gcgatatcgg ataaaggagg cgtaatggat ataacacctt tccttcatgc actttgtgct
     2221 gtggctgcgc aggtactggt tggtcttttt accggaaact gggcttacgg ggcgatagct
     2281 ggttgtacgt tcttcattgc gcgtgaacat acccaggcag aatatcgctg gattgaaatg
     2341 ttcgggcatg gcaagcgaat gaatatgccg tggtggggcg gttttgatcc gcgtgcgtgg
     2401 gatgtggcaa gcctgatgga ttttgctgtg ccggtggtgg cgtgtctgct ggtctggctg
     2461 ttggttaatc gtgggtgaaa aaaggtgagc tgtatatgca acggaggaag aaacctcgtt
     2521 gcgggaagcc tggaagaagt atcgggtgtt gctgaaccgt gttgatacgt caactgcaca
     2581 ggatattgaa tggccagcac tgccgtaggg taaaacatat aaattctata attagatgta
     2641 tctttccatt tacggcaagg aagggggctt ggaagacgta aggcatctca caccgagatt
     2701 attttttata tgtcaggtgt ctgaagtttt gctttggctc ttaaaatggt ttgccgcgag
     2761 gttttgaatt cccgggcaat ggcacttata cttacacctg acttaatccg ttcgaatacc
     2821 acctgtttct gttcttcatt taacacaggt ggtcgaccaa aacgtttccc tgcgccccgg
     2881 gctcttacta tcccggaatg agtgcgttca agtaaaaggt ctcgttcaaa ttcagcgact
     2941 gctgaaatta cttgcatcat catttttcct gttggactgg tcaggtcaat gccccccaat
     3001 gctaagcaat gcactctgat acctgtttcg gtcagttgtt ccactgtttt cctgatatcc
     3061 attgcattac aaccaaggcg atccagtttt gtcacaatca attgatcacc acatttcagg
     3121 cgagcaagca accggttaaa accaggacgc tcactggttg ctgctgagcc gctaatgtgt
     3181 tcttcgatta tttgctgagg tttgatttta aaacctgcac tttcgatttc ccggcgttga
     3241 ttttcggtgg tctgatccag cgttgatatc cgacagtaag caaaaattcg agacatagtg
     3301 agactctata cgaaattggt gctcatatca taatgcatct cagaaaataa ttttgattat
     3361 ttttgtgcat gtttgtatgt acacgttcga aaataaacga atgcgtatgc aaccccgtaa
     3421 ttttggtgag acccaaaatc gattttgtga aaaatggctt taactcggtt tgtttttcga
     3481 gttccgggcg gactcaagga agaagaatag tgttgcgtgt tattttaacc agatttcaag
     3541 ttgtttggtc gtggaaaagt ggagcaaaat gttgttaaag tggaaaaatg ataaaaaagt
     3601 aagtttatta tattacattt taccatttaa attttggttg tctttaagaa ctgatatcgc
     3661 tgtttgtaat aattctttgt tatccagcca tgattttttc tttatgtttc cttcaatgta
     3721 atcaagcaat gttctggtat tgataggtct tccctgtttt gctacttcca ctacagcatc
     3781 ccctaggata attctgactt caggaagctg cgcagggaac cactttaggg tgtcttttga
     3841 tttcatgaat atattcctta aaatattatt gattttcatt gcgatattgt atgtctgatt
     3901 caggatatgt tgacttatac atcggttttg tctgggttat tggatatgcc aatccctaat
     3961 tttattagag catgactaaa aatgctgaat atgataagga gcgaagtgat tatcagtatg
     4021 ctgttcatat agcctcgaat tagtaatgtg ttatatatga tatagttgac aatttttatc
     4081 ctgggtgttc ttaaagttcg tagataaaca ttgtcgtttc aggtatacag gaatgctaac
     4141 agatggcggc aaaaatcagg cggtttatgg cgcaagctga agtggcaact gcaaactatc
     4201 ttatgcagag actctacacg gattgggttt aaaagtatac atagataaca gtttttatct
     4261 gaagaagaaa aatatcaagg tgatatagcc tatatgcctt tgatgcggag gaatgaatgt
     4321 gatgggagtg atgtatctga atagttgaaa aaccgcagac acgccttatg caagaacgtg
     4381 ctgcggttgg ctggtaaatt tttcgatagt gcgagtattg aatgatttcc agccgttatt
     4441 gattttacgc gtaactccat gaaaaaacta cttatctgtt gaggagtttt tttggggcat
     4501 atatgggaca gaaataggcc ccagatagac actgagatcg aacttaggat gctttttaaa
     4561 aaatgcaact atctgaaaaa acctagaaaa cgccaaggaa accacaggat gggaaaaaac
     4621 acctgtgaat tatggatttc cagttatatt cgctcggcgc agcgttagtg tttcatgaaa
     4681 tattttttcc tgaatcatca acggcaatgg cgttaattct ggcaatggga acctacggtg
     4741 caggttatgt ggcgcgtatt gtcggagcat ttattttcgg caaaatgggc gacagaatcg
     4801 ggcgtaaaaa agtgctcttt attaccatca ccatgatggg gatctgtacc accttaattg
     4861 gtgtgctgcc gacctatgca caaattggtg tgtttgcgcc aattttgctg gtgacgctgc
     4921 gtattattca ggggctgggg gcaggcgcgg aaatttccgg tgccggcacg atgctggcgg
     4981 aatatgcacc aaaaggtaag cgcggaatta tctcctcatt tgttgctatg gggactaact
     5041 gcggaacctt gagcgcaacg gcaatctggg cctttatgtt cttcattctc agtaaagagg
     5101 aactgctggc gtggggatgg cgtataccgt tcctggcgag cgttgtcgtg atggtctttg
     5161 ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt tgagaaggtt aacgacagca
     5221 accaaccgac agcaaaacct gcacctgctg gtagcatgtt ccagagcaaa tccttctggc
     5281 tggcaacagg gctgcgtttt ggtcaggctg gtaactccgg tttaattcag actttccttg
     5341 caggctattt agtgcagacg ttattgttta acaaagcaat tccaacaggt gcattgatga
     5401 tcagttcgat tctcggcttt atgaccattc cgttccttgg ttggttatcc gataaaattg
     5461 gtcgccggat cccgtatatt attatgaata cctctgcgat tgtgctggcg tggccaatgc
     5521 tttctatcat cgtagataaa agctatgccc cgagcaccat tatggttgca ctgattgtga
     5581 ttcataactg tgcggtgctg ggattatttg ctctggaaaa cattaccatg gcagaaatgt
     5641 tcggctgtaa aaaccgcttt acccggatgg ctatttctaa agaaattggt ggtcttatcg
     5701 cttccggttt tggtcctatc ctggcgggta ttttctgcac catgacggaa tcctggtatc
     5761 cgatcgccat tatgatcatg gcatattcag tgattggttt aatctctgcg ctgaaaatgc
     5821 cagaagtgaa agaccgtgat ttaagtgcgc tggaagacgc tgcggaagat caaccgcgtg
     5881 ttgtcagagc tgcgcaacct tccataagtc tttaaatcct caatcccttc tcttaccgga
     5941 gaggggattt ttattcatat aacaaaacat gtagcttgcc atatttatat ttaaggaatt
     6001 gttatgggaa ataatttgtt atcagcaaaa gcgacactcc ctgtttatga tcgtaataac
     6061 ctggccccaa gaattgttca tttaggcttt ggtgcatttc accgtgcgca tcagggcgtg
     6121 tatgccgata ttcttgctac ggaacatttc agtgactggg gatattatga ggtcaactta
     6181 atcggcggcg aacagcaaat tgccgattta caacagcaag ataatcttta taccgttgcg
     6241 gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg tcgttaaaaa agccttgcac
     6301 gtacagattg atggcttaga aaccgtgttg gctgcgatgt gtgaaccgca aatcgcgatt
     6361 gtctctctga caatcaccga aaaagggtat ttccactctc cggcgaccgg acagttaatg
     6421 ctcgatcacc cgatggtcgt tgccgacgta caaaatcccc accagccgaa aactgcaaca
     6481 ggggtgattg tcgaggcgct ggctcgccgt aaagcggcag gacttcccgc atttaccgtc
     6541 atgtcatgtg acaacatgcc agaaaacggt catgttatgc gtgacgttgt cacttcctac
     6601 gcgcaagctg ttgatgtaaa actggcacaa tggatcgaag aaaacgtgac tttcccatca
     6661 acaatggtgg accgtattgt gcccgcagtg acagaggata cgctggcgaa aatcgaacaa
     6721 cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac ctttccgcca gtgggtaata
     6781 gaagataact ttgttgccgg acgtccggaa tgggaaaaag cgggagccga actggttagt
     6841 gatgtgctgc cttatgaaga gatgaagttg cgcatgctca acggcagtca ttcattcctg
     6901 gcgtatctgg ggtatcttgc cggatatcag cacattaatg actgcatgga agatgaacat
     6961 tatcgtcatg cagcgtatgc cttgatgttg caggaacaag cgccgacgtt gaaagtgcag
     7021 ggcgttgatt tgcaagatta cgctaaccga ttaattgaac gctatagcaa cccggcgcta
     7081 cgtcatcgaa cctggcagat tgcgatggat ggcagccaga aattgccaca gcggatgttg
     7141 gattctgttc gctggcatct ggcgcatgac agcaagttcg atctgctggc gctgggcgtc
     7201 gcaggttgga tgcgttatgt cggtggtgtt gatgaacagg gaaatccgat agaaatcagt
     7261 gacccactgt tacctgttat tcagaaggct gtacaaagta gtgccgaagg gaaagcgcgc
     7321 gtccagtcat tgctggcgat taaggcgatc tttggtgatg atttgccaga caatagcttg
     7381 tttactgcaa aagtgacgga agcgtacttg tctttattag cgcatggtgc gaaagcgacc
     7441 gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc atcggcggca ttcacacgct
     7501 tagttcatgc cgagacgaat caggtccaga ggtgccagtt tctcttcaca accttcaaca
     7561 actacggttt ttccgcggcg gatttgctca gcggtcagac cttcagtatc aatcgacagg
     7621 atcttgccag tgtgaccggt gccgctaacc ataacacggc tgccagtggt gattgcgtta
     7681 cggttacgat cgtaggtcgt cataggtgtt ttctcctttc tgatttacag tcgaagccca
     7741 cgtaacgggc gcaatataaa tactcctgtc gagtgaaata ttttttgttt ttgatcaagc
     7801 tcacatcttt tttgatgcat gcatgagcca taaggcgggg cgaatagact ctcaccccgg
     7861 atgggatgag aggaaatgaa ttactcttcg ctaaaccagt cgctgttttc ctgacggatc
     7921 tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca ttgcacgttc taccgcatcg
     7981 ccatcacgtt tttgcagggc agagaaaatc tcaagatgct ggcgtaacag catttctggt
     8041 ggagaaacgt ggtcgaaact catatagcgc acgcgatcaa cggtcgcttt caggttctca
     8101 atggtatccc acgccagttg acagtcggca atctgggtca ggagttgatg gaagttgtca
     8161 tcaagttcaa aaaaatcatc cagttgcttg cgctctatgg caatgcgttg ctggtgaaga
     8221 ttttgttcca gttggtagca ctggctttcg gtaatcatgc tcgccgcccg acgcgccacc
     8281 gcgcactcaa tggcctgacg gataaaactg ccgttgcgca cctgggccat ggaaattttg
     8341 ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc cgttttccgc cagtttaata
     8401 aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa cagaaacttc tttttccgac
     8461 aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc gacgaagaat acgataaatc
     8521 tgctgattga caggctgtgt gggattaagt tgcgtttcga cggtcattcg ttcttgcttg
     8581 tgagtgagtt aactgcatga gtctaccact tttttgtgac aagcaataac ccggcagtta
     8641 cgccgggtat aaaaattact gacggtggac attcagtccg gcatagcttt gggtaaccgg
     8701 catcatttcc agggtattga tattgacgtg agcaggcagc gttgacaccc accagacggc
     8761 ttcgctgaca tcttctggcg tcaatgcaac ggtattttga taggtttttt ccgctttacc
     8821 gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca cccaccagac ccggctcgat
     8881 gtcggtgacg cgcaccgccg taccatgcag gtccgtacgc agattcaggc taaactgacg
     8941 aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca tacggccagc tgcctgccgt
     9001 tgagccaatg ttaataatat gaccatgatt acgttcaacc atacccggta agacggcgcg
     9061 cgtcatatat actaggcctt tgttgttggt atcaatcatc gtttcccagt cttcaacgct
     9121 ggctttatgc gcaggctcca tgcccaacgc cagaccggca ttatttacca ggatatcaat
     9181 attgcaccac tcggcaggaa gcgatgccag catctcttca atagcggcgc ggttgcgaac
     9241 gtccagttgg gcgatataca gattatctcc cagttcgtct tttaactcct gcaaccgctc
     9301 ctggcgacgg ccagtggcga taactttatg cccttgttga ataaaacgac gagtaatgca
     9361 ttcaccaaaa cctgccgttg ctccagttac taaaacgatc atctcactgt tcctcaacgc
     9421 ttttgtgtgt caataacata gcacgtgatg cttaagagcg ggtaatactg gtttgcaaga
     9481 ttgcactccg ttatcagtaa gcctacgcta tggaacacct cgcgttcagg agaagaaaat
     9541 gacaacaatg aatcctttcc ttgtgcaaag cacactgccg tatctggctc cccattttga
     9601 tcaaatttcc aatcatcact atcgcccggc attcgatgag ggaatgctgc aaaagcgggc
     9661 agaaattact gccatcgcgc ttaacccgca aacgcctgat ttcaacaata ctattctggc
     9721 actggaacaa agcggagaat tacttacccg cgttaccagc gtcttttttg cgatgactgc
     9781 ggcgcatacc aatgatgaat tacagcgtct tgatgaacag ttttccgctg aactggcgga
     9841 actggctaat gatatctatc tgaacggtga attattcgcg cgggtagatg ctgtctggca
     9901 acgccgtgaa tccctggggc ttgatagtga atccatccgc ctggtggagg tgattcatca
     9961 acgttttgtc cttgccggag ccaaacttgc gcaagctgat aaagcaaaat taaaagtact
    10021 gaatacagaa gctgcgaccc tgaccagcca gtttaaccag cggttactgg cagcaaataa
    10081 atccggcggt ctggttgtga acgatatcgc gcagctggca ggaatgagtg aacaagagat
    10141 agcgctggcg gcagaggcgg ctcgcgagaa aggtctggat aacaaatggc tgattccgct
    10201 gctgaatacc acccagcaac cagcgcttgc tgaactgcgc gatcgcgcga cgcgtgaaaa
    10261 actgttcact gcgggctgga cgcgagcgga aaaaaatgat gccaatgata cccgcgctat
    10321 cattcaacgt ctggtggaga tccgtgcgca gcaggcgaaa ctgcttggtt ttcctcatta
    10381 tgccgcatgg aaaatcgccg atcagatggc aaaaacacct gaagcagcac tcaactttat
    10441 gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat gaattagcct ccatacaggc
    10501 ggttatcgat aagcagcaag gcgggtttag cgcgcagccg tgggactggg cattttatgc
    10561 cgaacaggta cggcgggaaa aatttgatct tgatgaggtg cagctcaagc catattttga
    10621 attaaacacg gtgttgaatg aaggtgtatt ctggaccgcg aatcagctct tcggtattaa
    10681 gtttgtcgaa cgttttgata ttcctgtcta ccatcctgac gttcgtgtgt gggaaatttt
    10741 tgatcataat ggcgtggggc tggcgttatt ttacggtgat ttcttcgccc gtgattcaaa
    10801 aagcggcggt gcatggatgg gcaattttgt tgagcaatca acgcttaatg aaacgcaccc
    10861 ggtaatttat aacgtttgca attatcagaa acccgctgcc ggtgagcctg cgttgttact
    10921 ctgggatgat gtaataacct tattccatga atttggtcat acgctgcacg gcctctttgc
    10981 ccgccagcgt tatgccacgc tttccggcac caacacgccg cgtgattttg tcgaatttcc
    11041 gtcgcaaatc aacgaacact gggcaacgca tccgcaggta ttcgctcgct acgcccggca
    11101 ttatcagagc ggggcagcaa tgcctgacga actgcaacag aaaatgcgta atgccagcct
    11161 gttcaataaa gggtatgaga tgagcgaact gcttagcgcc gcacttctcg atatgcgctg
    11221 gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat gatttcgaat tgcgggcgct
    11281 ggtggcagaa aatatggatc ttcccgctat accgccacgc tatcgcagca gttatttcgc
    11341 ccatattttt ggtggcggat atgccgcagg ttattacgct tatctgtgga cgcaaatgtt
    11401 ggccgatgat ggttaccagt ggtttgttga gcagggcgga ttaaagcgtg aaaatgggct
    11461 gcgttttcgc gaggcgatcc tttccagagg taacagcgaa gatctggaac gcctgtatcg
    11521 acaatggcgc ggtaaggcac ctcagattat gccgatgctg caacatcgtg gcttgaacgt
    11581 ataaacacca ccaatggctg gcctggagaa atcgccaggc cagcctggtt agagtagtaa
    11641 ttgcagcagc tgggcgaggg caaacctcac cgctaatgcg aatgccgttt cgcagtcgcc
    11701 agcaaaatta ctttttttgt atacccttcc gttataataa ttttcaaatt tataaatctg
    11761 attgttctga aatatgttta agactcttta atcttcttct tactttagtg agtaaaattc
    11821 tctgaatatt tgacagctag ttgcctgatt ttaaatgcct tataacaatt ggcagtattc
    11881 ttatttggca attataaaaa tagcaaccag acttaataag tcaaaccaaa cgcaattaag
    11941 gagttagtat gaaatttcag gccattgtat tagcaagttt tcttgtcatg ccttatgcgt
    12001 tggcagatga ccagggcggt ttaaaacagg atgcagcgcc accgccgcct catgcaatag
    12061 aagatggcta tcgcggaacc gatgatgcaa aaaaaatgac cgttgatttc gcaaaaacca
    12121 tgcacgatgg tgcttcggtt tcactacgcg gtaatttgat ttctcacaaa ggagaggacc
    12181 gttacgtttt tcgcgataag agcggtgaaa ttaatgttgt tattcctgcg gccgtttttg
    12241 atggacgaga agttcagccg gaccagatga tcaacataag cggcagtctg gataagaaaa
    12301 gcgcgccggc cgtcgttcgg gtcactcatt tgcagaaata aaaataaatt agcctgatgg
    12361 cctgaacta
//
LOCUS       DAPOZO010000126        12359 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPOZO010000126 DAPOZO010000000
VERSION     DAPOZO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.126"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..983)
                     /locus_tag="ROI21_004118"
     CDS             complement(<1..983)
                     /locus_tag="ROI21_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDX5112979.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVVKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1053..1445)
                     /locus_tag="ROI21_004119"
     CDS             complement(1053..1445)
                     /locus_tag="ROI21_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDX5112980.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(1632..1931)
                     /locus_tag="ROI21_004120"
     CDS             complement(1632..1931)
                     /locus_tag="ROI21_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDX5112981.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGLWSAWRLVKTTLKQQQLRG"
     gene            complement(1921..2325)
                     /locus_tag="ROI21_004121"
     CDS             complement(1921..2325)
                     /locus_tag="ROI21_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDX5112982.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(2328..2633)
                     /locus_tag="ROI21_004122"
     CDS             complement(2328..2633)
                     /locus_tag="ROI21_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDX5112983.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(2671..3039)
                     /locus_tag="ROI21_004123"
     CDS             complement(2671..3039)
                     /locus_tag="ROI21_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDX5112984.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLTEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3186..3569)
                     /gene="mzrA"
                     /locus_tag="ROI21_004124"
     CDS             complement(3186..3569)
                     /gene="mzrA"
                     /locus_tag="ROI21_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDX5112985.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(3573..4235)
                     /locus_tag="ROI21_004125"
     CDS             complement(3573..4235)
                     /locus_tag="ROI21_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDX5112986.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4580..5356)
                     /gene="exuR"
                     /locus_tag="ROI21_004126"
     CDS             complement(4580..5356)
                     /gene="exuR"
                     /locus_tag="ROI21_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDX5112987.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(5486..6784)
                     /gene="exuT"
                     /locus_tag="ROI21_004127"
     CDS             complement(5486..6784)
                     /gene="exuT"
                     /locus_tag="ROI21_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDX5112988.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            7267..8679
                     /gene="uxaC"
                     /locus_tag="ROI21_004128"
     CDS             7267..8679
                     /gene="uxaC"
                     /locus_tag="ROI21_004128"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDX5112989.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8694..10181
                     /gene="uxaA"
                     /locus_tag="ROI21_004129"
     CDS             8694..10181
                     /gene="uxaA"
                     /locus_tag="ROI21_004129"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDX5112990.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10264..10815
                     /locus_tag="ROI21_004130"
     CDS             10264..10815
                     /locus_tag="ROI21_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDX5112991.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPSAVDERG"
     gene            complement(10820..12064)
                     /gene="sstT"
                     /locus_tag="ROI21_004131"
     CDS             complement(10820..12064)
                     /gene="sstT"
                     /locus_tag="ROI21_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDX5112992.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         3115 a         3186 c         3182 g         2876 t
ORIGIN      
        1 ccgaagcgaa catctcgacc atgcggttca tcgagatcct gccacggacc aattgaaata
       61 atccccgtag ggttgatggt cttatggctg cggaagtagt gattacggat gtgatcgaaa
      121 ttgactgttt cggcgatccc cggcatctgg tagatatcgc gcaggaagcc atacagattc
      181 aggtaatcgc tgatgcggtg cttatcacac ttgaagtggg tgacatacac cggatcaaaa
      241 cgcaccagcg tggtccacag gcgaatatcg gcttcggtta gctggttgcc ggtcaggtaa
      301 cgatgctggc ctaaaatctg ctccagtcgc gccagcgatt caaacacttt cactaccgcc
      361 tcgtcgtagg cttgctggct ggtggcaaaa ccggctttat acacgccatt gttaacggtg
      421 tcataaatcc agccgttaag ttcgtcaatt ttcgtttgca gggctggtgg gtagtaatca
      481 cccgctttcg cgcccagcgc atcaaacgcg gtattgaaca tgcggatgat ttccgctgat
      541 tcgttgctga cgatggtgtg gttctttttg tcccagagta cgggaacagt aactcgtccg
      601 ctgtagtgtg gatcggcgtg gagataaagc tgatacagaa attcatgttg atagagtgta
      661 tcgccggttg cacccggaaa actgtcatca aaggtccagc cgttttccag catcagcggg
      721 ttcactacgg aaacggaaat aaacggttcc agtcctttga gtttacgcat gatcagcgtg
      781 cggtgcgccc acgggcaggc gagtgaaaca tagagatgat aacgatcttt ctctgcgata
      841 aaaccgcctg tgccagtggg gcctggcgcg ccatcggcag tgagccagtt acgaaatgcg
      901 gaagctgaac gttgaaattt accgccggta gatttggtat cgtaccaggt gtcatgccag
      961 acgccgtcaa tcagttgacc catatttctc tcctccaata gcaaaaacga ggagcttcct
     1021 cctcgttttt tatcgttaag tatagcgcgt gcttaccact ttttattcag caggcggtcg
     1081 atgctatacg cgcccggacc ggtaattgcc agcagcagga atccgccaga aattgtcagg
     1141 tttttcatga acatcagcga gttgacgcct tcagcaaagt tgctgtgaaa taaaaatgcc
     1201 gtcagcagcg taaagcccgc agtaaacagg gctgtggtgc gagtcaggaa accgaacagg
     1261 attgccagac caccaccaaa ctcaagcaga atcaccagcg gcagcataaa gcctgggaca
     1321 cccatcgctt ccatatattg ttgggtaccc gcgtagccag taatttttcc ccagcctgcg
     1381 gtaataaaca gaatcggcat taaaatgcgc gctaccagta caccaacatc ttctaatttt
     1441 ttcatcatac tctccactgc gccactcagg ctgctgattg tttttttgtg caagggcgcg
     1501 gtattagcgt cgttgctgtc gatggagaga atcataaacg tggtgaatga tgattgttag
     1561 caaggaaaac tgtcaaaaat cttcaaaaaa tttgagggat aaggccggaa tggctccggc
     1621 cagagaggtt attaaccgcg aagctgttgc tgcttgaggg tcgttttaac cagacgccag
     1681 gcgctccata agccaaaacc gcgtctggcc cagcggacca gcatattagg atggcgaatc
     1741 gtccagatcg ccatcacgct actgccaacc agcgcccagg agcgcagact tagcagcata
     1801 ttccagcgac gatcgtaagc gcctgttgcc tccagccatt cacgacgact ggcagaaaga
     1861 tccagccgtt gttgctggat ctggctaagc agttgcgcct tacgtcgttc acgttcgact
     1921 ttactgctca cgggactcct cctcgagcag ctgccgatcg tttgccagct catggcgtgt
     1981 atggcgcagc aaggtggatt tacgcgattt acgtaacgtc cagataccgc caatcagtgc
     2041 cagtagcagc aacaccacgg tggtggcaat catcgcattc aggcgatatt gcggatcaac
     2101 cgcccaaata atcagcacca tcaggctcat aagaccaaat gcagcgaaaa gcatcgtcag
     2161 gcccagcatc agtaaaagtt gaaagagatt cgctttttcc tcttccagct ccaccaccgc
     2221 cagccgcaga cgtgtttcca ccatttcaac catgatggaa acaattcgct gcccgatgcc
     2281 cagaacgctt ttaccgggcc cttgcgcgtg atgagtgtcc gccataatta acgacgcgat
     2341 agcagaacgc cgagcactac accgattgca gcgccaatgc ccacgcccgt ccatggattt
     2401 tcacgcacat actcatcggc acgcgccgcc gcgacacggg tttgtttggc gatggcatca
     2461 ccggtttcac ccaggcgata acggctctgt ttcagtgcct gctccgcttt gctacgaatc
     2521 ttactcaact cttctttcga cttctcgcca gatgagctaa gcacctcttc cagcgtatcg
     2581 gaaagggatt tcaactcagc acgcagatgt tccgtagtgt gttctttcga catagttttc
     2641 tccaggtgag taagtagtga ctattgtgag ttagtagtcg cgcgcttcca gctttttcag
     2701 ctcttcctgc gcttctgcca gtttccgttc gcgtttggca atcttatcgg catcgccttt
     2761 ttgcttcgcc tcggttaaat cttgctggcg ttccgccacc tcatctttct gctttgcgat
     2821 tttcttctga tgatcggcac gcagctggct atctgaacag ttggcccgga cttcactcag
     2881 ggctttattc agaccgtcaa tacgattctg gttttggtgt ttttcggcat agctgatctc
     2941 cttaaggata ttttgctcct tttcctgaca cagggtagtg gcataactac cggcactaag
     3001 agcaaagaga gaaacagcta aagcgatgcg gtatttcatt cttgaacctt ccattgtgat
     3061 tcggacctat tacaaaggaa cgattgtcca gacaacgaag cggtagtgtt aaccaccgcc
     3121 tcattaatca tagacagtta cggtgaaatc accaaagtca gtgatatgtt tcggataaca
     3181 ggaagttatc cgaagcgatg agagttatcc cgcaaccggg tcagccactg catagcctga
     3241 ctattattgt cctgctgcgc gatgatgtag ccatggggca atgttctgtc gaggaccgct
     3301 tttgcggcgg cactctggtc gctggagtca aaggtaatta acagagtgtc atttttgggg
     3361 gtgatacttt tgaaaggaat gccgtgagcg tcaaggtgat gccagattga aaaaccgtct
     3421 ggcatcgttg tcccttgatg aacggcacga atcgccagcg tagactcttg ctggcgaacc
     3481 gcggaccagg ccagcaacag tgtgccgacc agaagtaaaa cagcgccgga ccaggctagc
     3541 tggcgaagcg acatgcgagg tatttgcatc ccttaccccc gatttccata tttctttttc
     3601 cataacacga ccagagaacc tgccaggcca aacaccagca gcaccaccgg gagcagcatc
     3661 aggcatgaca tcagctggtc ctcgtacttt aaaaataccg gcgttttgcc gagcatgtaa
     3721 cccagagttg tcaggatcaa tacccacagc agaccgctca tccagttgaa gaactgaaag
     3781 cgcgcgttat tcagccctga taatccggca atcgtcggca gtagtgttct gacaaatgca
     3841 ataaagcgac caattaacag cgccgataaa ccgtgtttat gaaaaagatg gtgtgcgcgt
     3901 tgatgataat gcgcgggtaa atgagatagc cagttttgta ctgtgcgggt attgcccagc
     3961 catcgcccct gaatatagct gacccagcaa ccgaggctgg cggcaacggt cagcagcaga
     4021 atcgtttgcg gatagcccat cgcgcctttc gcaatcaata cgccgaccaa caccagtaaa
     4081 ctgtcgcccg gtaaaaaggc cgccggaagc aagccgtttt caaggaacaa aattacaaac
     4141 aagacaaaat acaacatgcc aatcatcgac ggattggcca gggtttcaaa atcctgcgcc
     4201 cacagggctt gcagcaattg ggtcaaaagt tccattcatt attcctggtg aatcggttaa
     4261 cgccacgctg gttttaaaag atgcactacc gcaatgttat tgctgttttg gtatgttttg
     4321 cgggtttgat acatcaacac agcctgaact attccctgtt aaatcgtgag ataagaaagc
     4381 attcaaaagc ataaattcaa attgaatcta attgtaacaa aggtcaacgt tgttcgttat
     4441 caaaattaca tgtccgtaga gtctgatttt gctgattgca ggggagggtg gcgagaggat
     4501 ttacacaggc tgacacttta tacttttcgc ttaccgacga agcaaacagg cgcattgcag
     4561 cgcctgtcgg atggaaattt catttgctgc cgctggtggc cgtatcgaga tgcacaaccg
     4621 ggttttctgc gaacagatag cggtcggcat tgaactcgaa atcgtcgctg gtttcgttaa
     4681 ataacatgat tttggtgttt tccagatgct gccacattgc cagcttagca gcatgaggat
     4741 ctttgcgaat cagcgccttg aggatttgat cgtggtcatc acaccagtta tcgacggtac
     4801 gggcatcaat gtgttcgtgc agttttttcc agtacgggtt atgactacgc tgggtccaca
     4861 ttttttcaac gatagccgcc agggcggagt tctgcgtcgc cagggctacc tgaatgtgga
     4921 actgcaaatc ccactcggaa tcacggaagc attgttcgcc gcgcgcctgc tcctggatgg
     4981 ccatcagttt catgatgtcc tgtttcgtta cctgagttgc cgcgaattcg gcaatattac
     5041 tttcgatgag ctggcgagcc tgaagcaact caaacggacc gtaattggcg aattccatat
     5101 tattgtcagc cgcctgctga tggcgcggct ggttggaaac cacatgaata cctgaacctt
     5161 tacgcacttc gacatagcct tcaacttcca gcatgatgat ggcttcacga accacggtgc
     5221 ggctgacgtt tttttcatcg gcaataaagc gctcagcagg cagtttatca cccaccagat
     5281 agacgccctg ttcgatgcgc tctttcagat cagcggcaag ttgttgatac aaacggcgtg
     5341 gttcagtgat ttccatatgc gctccaggca tgatacggca gactatattt gttataccac
     5401 ttttgcgagt ttagctctac tcacagtgat gaaaaagccg cctgagaagg cggctttgaa
     5461 gggaaactaa aacttacacg aagccttaat gttgcggtgc gggatcgtta tgtgattcct
     5521 gcgccacctc aatcgccggt ttgttctgca acacggtcca gataaccagt gcacctaaca
     5581 ggtcgaacac tgccagaact gcgaacagcg ggctgaagcc gatggtgtca gccagtgcac
     5641 cgactaccag tgcaaacagc gtacttgcca gccatgcgga catcccggtt aaaccgttcg
     5701 ccgtcgccac ttcgttacga ccgaacacgt cagaagagag cgtaatcagc gcgccagaca
     5761 gtgcctggtg agcaaaacca ccgatacaca gcagcataat cgcgacatac gggttggtga
     5821 acagaccgat catacccggg ccaatcatca gcacagcacc cagcgttacg accatcttac
     5881 gggaaacgat caggttcaca ccaaaccaac gctggaacag cggcggcagg taaccaccga
     5941 ggatacaacc gaggtcagca aatagcatcg gcatccaggc aaacatcgcg atctctttca
     6001 ggttaaagcc gtaaacttta aacatgaaca gcgggatcca cgcgttaaaa gtaccccatg
     6061 ccggttctgc caggaagcgc ggcagcgcga taccccagaa ctgacggtta cgcaggatct
     6121 gaccaacgga cattttcttc gctgtgtcaa cctggtgctg ggcttcctga ccattaataa
     6181 tgtagtcgcg ttcttcatcg gtcagatgct tctggtcgcg cggatgttta tagaaaatca
     6241 gccatgccat tgcccagata aagctcaacg caccggagat gataaatgcc atctgccagc
     6301 tgtgcattac gattgcccat accaccagcg gcggtgcaat catcgcacca atagaagaac
     6361 ctacgttaaa gtaacctacc gcgatggaac gctctttcgc cgggaaccat tcggagctgg
     6421 ctttcagacc cgccgggatc atcgctgctt ccgcggcacc gaccgcacca cgagcaacag
     6481 ccaggccgcc ccagctacct gccagcgcgg ttgcaccaca gaacacggcc cacagcacag
     6541 caaacattgc ataaccgatt ttcgtaccca gcacatccag tacataacct gctaccggtt
     6601 gcatgaccgt ataagcagca gaataggctg cgatgatata ggaatactgt tgggtggaga
     6661 tgtttaactc ttccatcaga gttggcgcag ctgccgccac agtgttacgc gtcaggtaac
     6721 caagcacggt gccgagcgtc accagtgcga tcatatacca acgtaaccct ttaattttac
     6781 gcatgtaaaa cctcatcgtt ttgttatatc cgcaggaagc gcggccatca tcccgccaga
     6841 ggctgaagat gttagttacg accgggtaac cgaaaaaaaa ccggcacgcc ccgaacgttg
     6901 ccatgaaagt ttcgtgctaa ttcggcttcc gtaccggtta acccgattca tctaacaaat
     6961 gaatgaatcg gcaatttgca ttccgtcgac ttatgatttg cgacggcaga aagataactt
     7021 gtcatacaac tttaaaaggt gagagccatc acaaatgtgg gaatatttgt agggacatta
     7081 cctgacgaca gcgaggccag tactggcgcg gcctgcagcg agatttacca ctttgagagt
     7141 aattttttta actacgttta ttgatctatc tcacgaaaat atcttcggac tctggaaatt
     7201 ggtgtgataa ctttgtcagc atcgcaccat aagcaagcta gctcactcgt tgagaggaag
     7261 acgaaaatga ctccgtttat gactgaagat ttcctgttag ataccgaatt tgcccgccgt
     7321 ctgtatcacg actacgcaaa agaccagccg attttcgatt accattgcca tttgccgccg
     7381 cagcagattg cggaagacta tcgttttaaa aacctgtatg acatctggct gaaaggcgat
     7441 cactacaaat ggcgtgctat gcgtaccaac ggtgtggccg agcgtctgtg taccggtgat
     7501 gcgtctgacc gtgaaaaatt tgacgcctgg gcggcgactg ttccgcacac tatcggcaac
     7561 ccgttatacc actggacgca tcttgaactg cgtcgtccat tcggtatcac tggcaaattg
     7621 ctttctccgt caactgccga tgaaatctgg aacgaatgta acgaactgtt ggcgcaggat
     7681 aacttctccg cgcgcggcat catgcagcag atgaacgtga aaatggtcgg cacgaccgat
     7741 gacccgatcg attctctgga gcatcacgca gagatcgcca aagacggctc tttcaccatc
     7801 aaagtgctgc cgagctggcg tcctgataaa gccttcaaca tcgaacaggc gacctttaac
     7861 gactacatgg cgaagctggg cgaagtttcc gataccgaca tccgccgctt tgctgatctg
     7921 caaactgccc tgaccaaacg tctggatcac ttcgccgctc acggctgtaa agtgtctgac
     7981 cacgcgctgg atgtggtgat gtttgctgaa gcgaacgaag cggaactgga cagcattctg
     8041 gcgcgtcgtc tggctggcga aaccctgagc gagcacgaag tggcacagtt caaaactgcg
     8101 gtgctggtgt tccttggtgc cgaatatgca cgtcgcggct gggtacagca gtaccacatt
     8161 ggcgcactgc gtaataacaa cctgcgtcag tttaaactgc tggggccgga tgtcggcttt
     8221 gactccatca acgaccgtcc gatggcggaa gagctgtcta agctgctgag caagcagaac
     8281 gaagaaaacc tgctaccgaa aaccattctc tactgcctga acccgcgcga taacgaagtg
     8341 ctgggcacca tgatcggtaa cttccagggc gaaggtatgc cgggcaaaat gcagttcggt
     8401 tccggctggt ggtttaacga tcagaaagac ggtatggaac gtcagatgac ccaactggcg
     8461 cagctcggtc tgttgagccg ctttgtcggt atgctgactg acagccgtag cttcctgtca
     8521 tacacccgtc acgaatactt ccgccgcatt ctgtgccaga tgatcggtcg ctgggtggaa
     8581 gcgggcgaag caccggcgga tatcaacctg ctgggcgaga tggtgaaaaa tatttgcttt
     8641 aacaatgcgc gtgactactt cgccattgaa ctgaactaag gtctggggtt gatatgcaat
     8701 acatcaagat ccatgcgctg gataacgtcg cggtcgcttt agcggatttg gctgaaggca
     8761 cagaagtcag tgtcgataac cagactgtta cgctgcgcca ggatgttgct cgtggacata
     8821 aatttgcgtt aacggatatc gcaaaagggg ccaacgtcat caaatatggc ctgccgattg
     8881 gttatgcatt ggcggatatt gcggcggggg aacacgtcca cgcccacaat acgcgcacga
     8941 atctgagcga tttggatcag tatcgctatc aacctgattt tcaggatctg cctgcgcaag
     9001 cggcagatcg tgaagtgcag atctatcgtc gcgctaacgg cgatgtcggg gtgcgcaatg
     9061 agctctggat cctgccaacc gtgggctgtg ttaacggcat cgcgcggcag atccagaacc
     9121 gtttcctgaa agagaccaac aacgccgaag gtactgacgg cgtattcctc ttcagccaca
     9181 cctacggctg ctcgcagctg ggcgacgatc acatcaatac ccgcaccatg ctgcaaaata
     9241 tggtgcgcca cccgaacgcg ggcgcagtgc tggtgattgg cctgggttgt gaaaacaacc
     9301 aggttgccgc attccgtgaa acgctgggcg atatcgatcc tgaacgcgtt catttcatga
     9361 tctgccaaca gcaggatgat gagatcgaag ccgggatcga gcatctgcat cagctgtata
     9421 acgtgatgcg caacgacaag cgcgagccag gcaaactcag cgaactgaag tttggtctgg
     9481 agtgcggtgg ttctgacggt ctttctggta ttactgctaa cccgatgctg gggcgtttct
     9541 ctgactacgt gattgctaac ggcggtacta ccgtactgac cgaagtgccg gagatgtttg
     9601 gcgcagagca gttgctgatg gaccattgtc gtgacgaagc aacgtttgaa aaactggtca
     9661 ccatggtcaa tgacttcaag cagtacttta ttgcccatga ccagccgatt tacgaaaacc
     9721 catcaccggg gaacaaagcg ggtggtatca ccacgctgga agacaaatca cttggctgta
     9781 cccagaaagc cggttccagt gtcgtggttg acgtgctgcg ttacggcgag cgtctgaaaa
     9841 cgccggggct gaacttgtta agtgcgccag gtaacgatgc cgtagcgacc agcgccctgg
     9901 cgggtgcggg ctgccatatg gtgctgttca gtactggtcg cggcacgcca tacggcggct
     9961 ttgtaccgac ggtgaaaatc gccaccaaca gcgaactggc ggcgaagaaa aaacactgga
    10021 tcgactttga cgcgggtcag ttgatccacg gtaaagcgat gccgcagttg ctggaagaat
    10081 tcatcgatac catcgttgag tttgccaacg gtaagcaaac ctgtaacgag cgtaacgact
    10141 tccgcgaact ggcgatcttc aaaagcggcg taacgctata aagtcgaaat gagttgttaa
    10201 taaaaaacgg cgtttcataa tgtgaagcgc cgtttgcttt tccatacctg ctaaggattt
    10261 atcatgaccg cgtattggct ggcccagggc gtgggtgtca tcgcctttct gattggtatc
    10321 acaacatttt tcaatcgtga cgaacgtcgc ttcaaaaagc agctttcggt ctatagcgcc
    10381 gttattggcg tacatttttt tcttctgggc acctatcccg ctggtgccag tgccatcctt
    10441 aatgccattc gtacattgat taccttacgc acgcgcagct tatgggtaat ggcgattttt
    10501 attgtgctga ctggcggaat tggcctcgcg aagttccatc atcctgtcga actattgccg
    10561 gttatcggca cgattgtcag tacctgggcg ctgtttcgct gtaaagggct gaccatgcgc
    10621 tgcgtgatgt ggttttcaac gtgttgctgg gtgattcaca acttctgggc ggggtcgata
    10681 ggcggcacga tgattgaggg gagttttctg cttatgaatg gcctgaatat cattcgtttc
    10741 tggcggatgc agaaaagggg gattgatccg tttaaagtag agaaaacccc ttccgccgta
    10801 gacgaaaggg gttaaacaat taattacgca gggcgctatt tgccagacga tcgtcttccg
    10861 cctggcaagc tgccgcagtg aacagcacgt cagttgaaga gttcagcgcg gtttcgcaag
    10921 agtcctgcaa tacgccgatg ataaagccga cggcaaccac ctgcatggcg atatcgttcg
    10981 aaataccgaa catattacag gccagcggga tcagcagcag agaacccccc gccacgccgg
    11041 atgcgccaca ggcacacaga gaagccacaa cgctcaacag cagcgctgtg ggcagatcga
    11101 ccggaatacc cagcgtatta actgcagcca gcgtcaacac ggtaatggtg attgctgcgc
    11161 ccgccatatt gatggtggca cccagcggaa tagaaacgga ataggtatcg cgatccagat
    11221 tcagcttttc acacagcgcc atattcaccg gaatgttcgc tgcagagctg cgggtgaaga
    11281 aggcatacac accgctttca cgcaggcaca gcagcaccag cgggaacggg ttacgacgaa
    11341 ttttccacca caccagcaat gggttaacca ccagcgccac cagtaacata cagccaacca
    11401 gcacgaccag cagttgcgcg tagccccaca gtgtggagaa accggtggtt gccagggtag
    11461 aagaaaccag accaaaaata ccgatcggtg cgaagcgaat gaccagtttc accataaagg
    11521 tgacggcatt cgacatatcg ttgaccaggt ttttggtggt ctcgttaccg tgacgcagtg
    11581 cgaaaccgag gccaattgcc cacaccagaa tcccgatgta gttacctttc agcagcgcgt
    11641 cgatggggtt ggaaaccatg ctcattacca gcccgcgcat cacttcgaca atgcctgacg
    11701 gcggcgaaat atcacccgcg ctactggaca agtgcagggt agaagggaag gcaaagctga
    11761 agactactgc ggccagagca gcagagaagg tgcccagtag atagaggaac aaaataggac
    11821 ggatattggt tttctgcccg tgctggtggt tagcaataga cgccattacc agcatcaaca
    11881 ccaggatggg ggcaacggct ttcagtgcgc cgacgaacaa agtacctaac agaccaacag
    11941 cttccgccgc gggttttgag atccatgcca gaagaatccc cagaacaagg ccgaccagga
    12001 tttgttttac caggctgcca tgagccagac gccggaatag ccccggtgaa cgttgcgtag
    12061 tcatttttcg atcctttcat tgtgttgtcg cacatccctg gtgcgttctg tcgacgcatc
    12121 tttcaggatg taacaaagtg tttgcaaggt cgagtataag gaaagttaca gcttcgggaa
    12181 gcgaaaaatg ctggatttta aagagaggtc atattttgca acttaatggt tacatttatt
    12241 tgacataaac agagggaagg actggctact aaggctgatt agataacata tgggcaaaac
    12301 tatttggaat atgagaacgg ttgcgctcct ttaatgtgct atgccatata aagtcaatg
//
LOCUS       DAPOZO010000127        12269 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPOZO010000127 DAPOZO010000000
VERSION     DAPOZO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.127"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..622
                     /locus_tag="ROI21_004132"
     CDS             <1..622
                     /locus_tag="ROI21_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5112993.1"
                     /translation="LLMLLRLPGTPPQPTPEYKSVSLKRDCLPYLLCAILLAAAVSMM
                     QLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGVLRPQRLTPVALLLSAGV
                     LMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLNDKLADGAGAGWLATSHTL
                     GYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWHYRTIK"
     gene            complement(781..1767)
                     /locus_tag="ROI21_004133"
     CDS             complement(781..1767)
                     /locus_tag="ROI21_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5112994.1"
                     /translation="MYHHDFNEKIGFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            1867..2601
                     /gene="nanR"
                     /locus_tag="ROI21_004134"
     CDS             1867..2601
                     /gene="nanR"
                     /locus_tag="ROI21_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDX5112995.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(2637..3572)
                     /locus_tag="ROI21_004135"
     CDS             complement(2637..3572)
                     /locus_tag="ROI21_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX5112996.1"
                     /translation="MGFIMNKDRSLNEMMLSLFNKSTIVDLSSETISESKSLSGRIYQ
                     DPEHNGEFELDRVLTDIYAPQLIIFLPDKPNGTGILIIPGGGYQAVMLDKEGTALAPE
                     FLSQGYTLFVLSYRLPSPVEGENINKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFS
                     AGGHIASTFVNQICDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPGSRKQLLG
                     DNSTSENIIQYSADLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHL
                     YEKGGHGFSIQWATDLPVGGWIKLFMDWMYSHGFK"
     gene            complement(3622..4732)
                     /locus_tag="ROI21_004136"
                     /pseudo
     CDS             complement(3622..4732)
                     /locus_tag="ROI21_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
     gene            complement(4745..5455)
                     /locus_tag="ROI21_004137"
     CDS             complement(4745..5455)
                     /locus_tag="ROI21_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HDX5112997.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKIN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYK
                     GKDNKHENSYRVGVLINF"
     gene            complement(5501..6331)
                     /locus_tag="ROI21_004138"
     CDS             complement(5501..6331)
                     /locus_tag="ROI21_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDX5112998.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTGDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFY
                     "
     gene            complement(6335..7807)
                     /locus_tag="ROI21_004139"
     CDS             complement(6335..7807)
                     /locus_tag="ROI21_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5112999.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAEE"
     gene            complement(7856..8731)
                     /locus_tag="ROI21_004140"
     CDS             complement(7856..8731)
                     /locus_tag="ROI21_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDX5113000.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNKLAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYVQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            complement(8765..9682)
                     /locus_tag="ROI21_004141"
     CDS             complement(8765..9682)
                     /locus_tag="ROI21_004141"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDX5113001.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPR
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLFEEHDRNKKMKII"
     gene            9800..10198
                     /locus_tag="ROI21_004142"
                     /pseudo
     CDS             9800..10198
                     /locus_tag="ROI21_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547016.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            10344..10769
                     /locus_tag="ROI21_004143"
     CDS             10344..10769
                     /locus_tag="ROI21_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDX5113002.1"
                     /translation="MEQKILSAEPRRTFSNEFKLQMVKLASQLGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSDAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            10766..11116
                     /gene="tnpB"
                     /locus_tag="ROI21_004144"
     CDS             10766..11116
                     /gene="tnpB"
                     /locus_tag="ROI21_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDX5113003.1"
                     /translation="MISLPSDTRISLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRSDMIKILWADADGLCLFTKRLEEGLFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            11147..12244
                     /locus_tag="ROI21_004145"
                     /pseudo
     CDS             11147..12244
                     /locus_tag="ROI21_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         3610 a         2968 c         2312 g         3379 t
ORIGIN      
        1 gctgctgatg ctgctgcggt tgcccggcac accaccacag cccacaccgg agtacaagag
       61 cgtcagtctg aagcgggatt gtctgcctta tctgctttgc gcaatattac tggctgcggc
      121 ggtgagcatg atgcagcttg gactttcgcc tgcccttact cgccagttcg ccacggatac
      181 caccgccatt agccaacagg tggcctggct gttgggtctg tccgcagtag ctgcgcttat
      241 cgcgcagttc ggggtactcc gtccgcagcg cctgactccg gtggccctgc tcctgagtgc
      301 cggagtgttg atgagtggtg gtctggctat catgctctcc gaacagctat ggttgtttta
      361 cccgggctgt gcagtgctgt catttggagc agctctggca acacccgctt atcaacttct
      421 actgaatgat aagctggctg atggcgcagg cgcgggctgg ctcgctacca gtcacacact
      481 tggctatgga ctgtgcgctt tgctggtgcc gctggtatcg aaaacaggtg tcgcaatcgc
      541 gctgattatg gcagcattat ttgcaactat attatttacc attgtgtctg tatttatctg
      601 gcattaccgt actatcaaat aaagatatga tcatcacctt actggagtaa atcagaggat
      661 atgagagagg acctgcgtct gatgaaagca tgtcaggcag tgttctggct tgctgccccc
      721 gccactccgt catgtctctc agagaatcat gtcagagctg tagatatatc ctgaacttgt
      781 ttacaaataa tttcttattt tttgcgcata acattcagca ataactcccc ttctggccat
      841 tgaattaaaa tgtgtacttc tctgtgacgt tgtccagctt aattctgtcc tccatgatga
      901 ccccagatac ccgacagata aatcatcggc atcttcttct ggctcattag ttaattcatg
      961 tcggttacta tcctgaaagc tgagaaaatg tatttcatca tcagtccgtt gtctgtaatg
     1021 tccatatata aaatcatatt ctttttgata agattccttc cagtaccagg ttgtatcacc
     1081 acacaaccat ggaactcttt ctgacgaatt accaacacac tgagatgagt attcagataa
     1141 ctcagacctg aaagaagtta ccatctgata aaacaagtca ggatgctttt cataatctgg
     1201 tgacataaga tcaaactccc cctgcatcca gcagacagat agcagtttat tctttctgtt
     1261 atattccaga gctaccttaa cccgggctac taagtcctca tagagtgctg ttccggtgcc
     1321 ccaccgcatt gcatcagctg ttgcccctgt atcaggtaca tacatacctt cagctccaac
     1381 agtaaaggcg gcacctcctc tgcaacaagg aaccagtaat atccctgctt gctcaggtat
     1441 gtatggcagt aactttttag ctatatgcaa tccctgtccg acacaaccat attccccttt
     1501 atgcaaatca gcagccggat ggtgatatcc actcatatcc tgaacatcat gcaggcagtg
     1561 atctgccgga ataatctcat tgaatttaca ctcttttcct cctggtgtga ttgtttttcg
     1621 acgagccagt tgttttacac gggactcggg tttgtcgagt gtatctggca ggggaatacc
     1681 ttcaccgtag gccataccat tagactgtcc tgcgagggct atcacatacc agaaacctat
     1741 tttttcatta aaatcatgat gatacatcat tcccccttta cataatagat ttaattaaat
     1801 attttcatta ttaaaagaaa gtgttaaatt aaaaactcat ttgctctaat ccatataaat
     1861 caatatatgt ccgacaacaa aaaaaccgtg ttacccgggc taacaacgac cagaaagccc
     1921 attgttacaa aaaaactttc agaaatcgtt gccgaagagc ttgaaagaat gatccgccag
     1981 gaggagataa gggaaggtga atacctgcct tctgaacgtg aacttatggc attctttggc
     2041 gttggaagac catctgtcag agaggcttta tcatctctca gacataaagg gctaatccgg
     2101 tataacaagg gagaaaaagc cagtgtctgc cgtccatcac cagaaaccat cattaatggg
     2161 ttatcaggca ttgttaaaga ttttttatct acagctgatg gcatcaagga ttttgaaaga
     2221 ctacgcatct tttttgaaac caaccttgta agatatgcag caaaaaatgc aacgcctgag
     2281 gatattcaca ggcttgagaa ggtgctcagt atccatcata actcactgag aacccccgaa
     2341 gtgtatgctc aatatgatat tgaatttcat agtatacttg ctgatatacc tgacaataaa
     2401 gtattaaaaa caatacactc agcattttct gaatggctga taatggccaa acctctggaa
     2461 aagaataaaa tgacatatga aaataacaaa aaaacccatc agcagcatat tgaaatactt
     2521 acggccataa aagagcatga ttgtgataaa gctgaacagc taatgtataa tcacctttat
     2581 aaacgaattg aagcatcata gttactcgac gcttcaccag gtagtcacca taaatattat
     2641 ttaaatccat gagaatacat ccagtccata aacagtttta tccatcctcc aacaggaaga
     2701 tctgtggccc actgaatact aaaaccatgc cctcctttct catagagatg catttcagca
     2761 gaaacacctt tctctattag gccacggtac atgagcagac tgttttcaat tgacacaaga
     2821 tcatcatcac aacaatggag taaaaacaca ggaggaaaac atgatgtgac attaagatcc
     2881 gcactatatt gtattatatt ttctgaggta ctattgtccc ccagtaattg cttcctggag
     2941 cctggatgtg tcacatcatc tttcattgat attacaggat aaattaaccc gacaaaatcg
     3001 ggaatagcga aaatctcttg ttctttactg tcagaagaac gtttgaaatc acaaatctga
     3061 ttcacaaacg ttgaggcgat atgcccccca gcagaaaatc ctaatacacc aatacgatga
     3121 cgctgtagat gccatttatc agcattctta cgaatataaa gcattgcaga tattgcatca
     3181 ataaaagttt tgtttatatt ttctccctca actggcgatg gtaatctgta actaagaaca
     3241 aataatgtat agccctgaga taaaaactct ggagcaagag ctgtaccttc cttatctaac
     3301 ataacagcct ggtagccccc cccgggaata atcaatattc ctgttccatt tggtttatct
     3361 ggtaaaaaaa taattaactg aggagcataa atatctgtca atacacgatc aagttcaaat
     3421 tccccgttat gttcaggatc ctgataaata cggcctgaaa gagatttact ctcagatatt
     3481 gtctcacttg acaagtcaac aatagttgat ttattaaaca agcttaacat catttcattt
     3541 aaagatctat ctttattcat tataaacccc attaaaataa ctcaggtgac tgaacaacta
     3601 atacagtcac ctgcattaca gttatttaac aatctttatt tgtttgtcat tttttttcag
     3661 ataaataaca tctgaaacag ctttaccatc ttttttctcc ccccccagaa tgagcatccc
     3721 ccccctgcca gggcaggctt acaccataag caagtccttc cggcaaagaa ccaacttttt
     3781 tccagtcatt ttttattagc tgatatatct cttttgaata ggctttatcc agtcctttat
     3841 gagaccagta ttcaccattg ctatactttt ctgccgctcc cgggaaatat gcaccaccgg
     3901 ccagaaagat atttccatca ataacagagg catatgctcc tgacaccccc tcgggtgaag
     3961 ctactggtgg aagttcagac catttaattc gatcatgcga aagctccccc gtccaggatc
     4021 taacagaacg aagccccggt tttctctccc ctcccagtat ataaatttgc tgttctgcaa
     4081 aaatcaccga tgaaccagca gttcccggaa atggagtcgt tcccaggttc ttccactggt
     4141 tttttgacgg gtcataagaa attatatgat tatttaaaaa ataatcatca gcaggtttac
     4201 taaaataatc cctgttgact ttctctgtca gcgcggaatt cccttttgat aactcgacat
     4261 ctgaaaagta tccattaaaa atattctcat ttacaccacc agtagatacc gcatgaccat
     4321 tatggataaa agaaacatga ccggcaagag atacaggggg acgggtcatc aatttttccc
     4381 atttatcttt agcaatatca taggaataga catctttttg taaggttatg actccacttg
     4441 tatcttttcc aattcctcca aatacataga tcttaccatc aataatagta gctgttgcct
     4501 gctctctggg aactgtcggc cattgcgcta tcttagtcca ttttttttca ctctggtttg
     4561 tatctatcat ataccagtta tttcccagac ttcccagtcc tatatatatt ttaccattct
     4621 cgatccctcc tacaccatat ttaaatggtt caggaatttc cggtaaagct ccggctaccg
     4681 acgttccaat aaacaagcag aagggaagat atataaagtt tttcactttc attgtaatca
     4741 ccttttaaaa attaatcaga acaccgacac ggtaactatt ttcatgttta ttatctttcc
     4801 ccttatacac cccttgttta tccaagtaat catattctat atatggggtg aacaccggtg
     4861 acattttata ttgcagaaca aatgcatttt cagttgctgt ttttttatga ttcccatatt
     4921 tgaaattatt aaccttcgta tataccgtgg tctgccatgc aaacaaccat gaatcattaa
     4981 ttttgtatgt cacataacca tcaaactgat gctgatcatt acgcgccctg ttgccatcta
     5041 aatccgtctg tcggaaagct ttccagtcat agcgatagcg taaggccaga ttaagatctg
     5101 gcgttgcctg atacgaaatt ttcagataag ggttatactg acttcctgct gacatccatt
     5161 gtgtctgaat ccctgggatt agagcccatt tgtcatctat tttatatgtt ttatttatat
     5221 ctattttatt ataattgagt gcaaacaatt cattttcatt gttgtgaatt gtattccagg
     5281 tgtttgactc aaaacttgcc caccaaccat tattccaccc ctgactaacc tgaatcaacg
     5341 tttcataagc gtgactggcg cttctgtatc ccccacgtag attaagagtt aatgcctgag
     5401 agcacaaagg taaaccaaga agaattacag ttaatagtgg ataatatttt ttcataaatg
     5461 cctcatcatt aaatatattt atcacttgta atttgatttc tcaataaaac ttatgttccg
     5521 caagccattc cagaagcagg ctttgccatt tttctatcgg atatccattt tttccggata
     5581 caccaaatcc atgacctccc ttatcaaaaa aatgagcttc tgcatgatgt cccttatccc
     5641 gaatagagtt aaaatatctc agagaatgaa taactggaac atccttatca tctgttgcat
     5701 gcacaataaa gactggtggc atatactctg taacactatt ttcaagactg gctgaatatt
     5761 cactttcacc tgcatccaga agacgtttcc ggcatccctg atgagcaata ctcctgtcaa
     5821 gagatataac tggatatatc agagccatcc agtcaggacg cacagaagtc tcttcctcat
     5881 aatggagatg agtaatatat ccagcaagat gaccacctgc cgaaaacccc atacatccaa
     5941 cactcttaaa tgttctctcc gacattccgg cccagtaaga aataaagttc agggcattct
     6001 tcgcatctgc aaaggaataa taaccattaa cggagggcat tctgtaagaa agaataaatg
     6061 ccgtatatcc ctgacctgct atccatctgc caacatcaag tccctctctt tcataaacaa
     6121 tcctcctgta tccacctcca ggtataatca gcacactatg ttgattagat acaacaggct
     6181 caacaactgt aataaggggc acaatacctg tatatattat gccatcgcca gtattctcaa
     6241 atagcgaact gtcagttata taacgctcat aattatcaag agaatatata ttctccctgt
     6301 aatgaatcat ttttttattc cctgatagca tccgttattc ttctgcaaca ttcttttttc
     6361 ttgctccata aaaaggtttt ccatcgacag tatcatattc aagcgcagct tctggatgga
     6421 tccatctctg tacacgtgat ggcatatcaa aaccaataag gacaagaaca acaaatgtta
     6481 agataaaagc aagagaacac attgctgtac caagacttgt atgagacgca acaactgcac
     6541 caagaatagg cgcacatgct ccaccaagtg aacctacatt ataaataaac cccagtcctg
     6601 ccgcacgctg ctccacatta aaataaccac tgattatttt cggcaccaga gcaccaatcc
     6661 cctgaccaaa aacctgctga gtaaagatta gaaggccaag taaccataca tagtcccggt
     6721 ccacaagaaa aacggggata ataacaagct gaccgataag aagtgatata acatatgctt
     6781 ttcgggttcc aagccagtcc cccataaaac cacctataca acttccagct gcagagccaa
     6841 aacctgccag cataaatatg agagcaacag tttctggtga atactgcata tcaaccttaa
     6901 gccatgtagg aagaaaagcc tgcagtggcc atccgtagag gaaacagaac ataaccacaa
     6961 gcatcaacat aatgcctgtt ggccatcttt tccccatacc ctgatatata aaactgataa
     7021 aaacagccgc acagcacaat gccataactg tgattaaagc agcgtttgca gtgacgcctg
     7081 aaaaacaaac atatagacat gcaaatgcaa tacaagacag aagaatattt atgtatttat
     7141 tcttttctct gtacagaata tccaccattg ttctgacggg tttattctct tgttgccttt
     7201 tttgccagtc atcagactcc ggaagattct tgcgaagata aaaagcaaat aatattggaa
     7261 gcatgccgac aaagaaaaga gaacgccagc cccatagtgg tactacaatg ctataaacct
     7321 gtgcagctaa acctccccca atagaaaatc cagaaattaa aaaaccactt gccttgtttc
     7381 tgagatgaac aggccagctt tcgataacat atgttacact tgagccatat tcacctgcca
     7441 taccaagccc aataacaatg cgagcaataa acatcactgc atatccagga gccagaccac
     7501 aggcaagggt tccgagagag aatagcacta tacttaaaac cattgccatc ctgcgcccca
     7561 tcttgtccga taaagcgcca atagcaagcc ccccaaacca tcgagaaata aaagcagcgg
     7621 aaataaggct tgccgcttct attgtcgtca agccgaattc atgctgaacc tctgtaagaa
     7681 ccagagatat cagaacgaaa tcaaaaccat caagtaaata acctgtccat gccgcaccga
     7741 atatcttcca ctgtctgggt gaaagatgct tataccaaac ctgctttttc tgaatacagg
     7801 agtccatatt aaacctcaca ataatagcgg atttataata cactggagta ttactttaag
     7861 taatacaatc ccggtcccaa agagttgcac caataagacc agagtcactt ctgaaatatg
     7921 ccggaaacag cattacatta caaatttcag aatatatgga aagatgtttc gacactttct
     7981 gaacataccc atcagcaagt ccaacacttc ctccgacaac aacaacctga caatcaagca
     8041 gtaattttaa acttactatt aattcagcaa tagtactggc agaacgctct gtcaaaaatt
     8101 ctgcttcctt atatccctgt ctcgctaatt caaaaatata tttggtgcta tatcctgcaa
     8161 gtttattttt tgctgccccc atgatggccc gaccagatga aaccgcttca acgcatcccc
     8221 gacgaccaca tccacattct gtatccgtta ctccggacaa tatatgccca acatgtccgg
     8281 ctaatccaga aacacctgtt aataattttc tgttaactac aattccccct ccaacccccg
     8341 tagaaacggt tataaaaacc atatcacaaa tttctttcgg caaaactgta tattctgccc
     8401 atgcggctgc ctgggcatca tttatcacac ttccatttaa accagttata tcttccatta
     8461 tatttttcag aggatactct ttcagccctc caagattgtc tggattcaga gctgtcaata
     8521 ctccattatt aataatccct gttgatgcta ctgcaaatcg ctccgctgtt cccttcattt
     8581 cagaaaccag tagacgtaaa gcccctgtca tttcgtcagg acatctttca tgaggtgttg
     8641 atatctgttt tttcagcaaa aatgaaccgt catcagatat caacgccgct gaaattttcg
     8701 ttcctcctat atcaacagcc agtgttatca ttcattttca cctatataac aatcttgcaa
     8761 ttattcagat tattttcatt tttttgtttc tgtcatgttc ttcgaacagt cgctctgcaa
     8821 gttttgataa tacatccaga ttcttttcct ttaccggaga aaaaggactt ctgcatacag
     8881 gagtatttat aatccccata tagtaaagaa gagtttttat acctgccagg acaccactct
     8941 gaataagagt gtcaataacc tggttacagg ctacttgcat ttccttcgcc ttaatcacat
     9001 cattgttttt gactgcttca aatatttcaa gataacgcca ccccattata ttatatgtac
     9061 taccgattcc cccatctgca cctgcaatta accctgatgc aaatatttca tcgtaaccat
     9121 tatataacac aagctctgga tgattccttt tgatttgttc catctggaat aaatcaccgg
     9181 atgtctgttt taatgcacaa accctgggta ttgtgacaag ctcatttatc tgatctaaag
     9241 aaaaacgcac tccgctaaga gccggtatat tataaactac catcggaagt ccatctgcag
     9301 aatcaataat cttcctgtaa tggatacaat gctcttcaaa tgaaaaagga taataaaatg
     9361 gagtaacagc agatatagca tgatatccgt atttttttgc agcttttgca agtacctcgc
     9421 tttctgccgt actgatgccg cccacatgcg ctataagtgt cagtcttcca tctgattcat
     9481 cggctactgt ttcaagaatt ttttctcgct ctgcaacatt ctgaagaaat gcttctccag
     9541 tagagcctcc aacatacaat cccttaatat tttgttcgat attaaaacgg atcagttttc
     9601 gaagactctc catatcgatt tgctgggatt ggtcataagg agtgggtaat gctgatatga
     9661 cccctttaaa ttcacattgc atttttaatc accttcactt aactgcataa tgggatacct
     9721 agtacaccat caagtacaaa gattatccaa cagaaaagaa gcacaatgtg aagattatca
     9781 aaaaaaaacc atcagaaagc tgtcatgccg ctcagttaag gatcggttga tcgatccagt
     9841 gtccagttac tattctctgc gtaaccctct gcgactatct tgccccggaa ctgattaccc
     9901 acagccctga agagtccgac actgtcacgc tacgcaaacg gcgtcttcct ctggaaatga
     9961 tggcctggtg aattatcagt atggcccatg agcgtaagga accttcacca gattgtgaac
    10021 cggctggaca catgctgcca ggtagtcgcc cctttgtcgc gccgtgatcc atgcccagca
    10081 gcggccagga agcggggccg tacgccacgt gttcacgtaa acagcgcagc cctgacataa
    10141 caccacgctg tatccgctct ggtgcaatat gaacctgctg accatcgatg gcgtgttcta
    10201 actaacacag gacacgccag aacacgacat aaagcttcac tcgccagata catggtaagc
    10261 gtaaactgac cgccgtatgt agccattaag ccagtattgg taacgtaggt gcccaccttt
    10321 tcatcaacta gtggacacct gttatggaac agaaaatatt atccgcagaa ccccgcagaa
    10381 cattttcaaa tgagtttaaa cttcaaatgg ttaaactggc ttcacaacta ggagcctctg
    10441 ttgcccgtat tgccagggaa cacgatatca atgataacct cctgttcaaa tggctcaggc
    10501 tctggcagaa cgaagggcgt atatcgcggc gtcttccggt aacaacctct tctgacgctg
    10561 gcgttgaatt attacctgtt gagataacgc ctgatgagca gaaagagcct atggcggctc
    10621 ttactccgct tttatccact ccttcacaaa gcacagtcag tgccagttcc tgtaaagttg
    10681 agttccgcca cggtaacatg acgctggaaa atccttcacc agagctgctc acggtgttga
    10741 tccgtgaact gaccgggagg gggcgatgat ctcgctccca tcagacactc gaatctcgct
    10801 ggttgccggc gttactgata tgcgtaaatc cttcaacggt ctgggtgaac aggtacaaca
    10861 tgtgctggat gagaacccct tctccggtca cctgttcatc ttccgtggcc gacggagtga
    10921 catgattaaa atcctctggg ctgatgctga tggtctgtgc ctgttcacca aacgcctgga
    10981 ggaaggcctg tttatctggc ctgcggtgcg tgacggtaag gtatccatta cccgctcgca
    11041 gctggcaatg ctcctcgata agctagactg gcgtcagcca aaaacatccc gtcttaacgc
    11101 actgacaatg ttgtaaaaat gtcatggccg gattataaaa acggtcatga gtcggaaata
    11161 cctcattcgt atcactgagc tggaaaggct gctctctgag caggctgaag ccctccgtca
    11221 gagagatctg caactgagtc tggttgaaga gacggaggcc ttcctgcgct ctgcactggc
    11281 acgtgccgaa gaaaagatcg aagaggagga gcgggaaata gaatatctga gggcccagat
    11341 agaaaaactg cgccggatgc tgttcggtac ccgttctgaa aaactgcagc gtgaggttga
    11401 acaggctgag gcccaactga aacaacgcga acaggaaagc gatcgttaca gtgggcgtga
    11461 ggatgacccg caggttcccc gccagttgcg acagtcacgt catcgtcgtc cgttaccagc
    11521 acatcttccc cgtgaaatac accgcctgga gcctgaagaa agttgttgcc cggagtgtgg
    11581 cagtgagctg gattatctgg gtgaagtcag tgctgagcag ctggaactgg tgagtagcgc
    11641 cctgaaagtg atccgcactg ttcgggtaaa aaaagcctgt acaaaatgtg actgtatagt
    11701 tgaagcgcca gcgccgtccc gcccgatagc gcgcggcatc gcggactccg gattacttgc
    11761 ccgcgtgtta acgggaaaat actgcgaaca cctgccactg tatcgtcaga gtgaaatctt
    11821 tgcccgacag ggtgccgaac tgagccgtgc attaatctcc aactgggttg acgcgtgctg
    11881 ccagttaatg actccgctga atgatgccct gtaccgttac gtgatgaaca cccgcaaggt
    11941 tcacactgac gacacaccgg taaaagtgct gacaccgggc agaaaaaagg caaaaacagg
    12001 acgcatctgg acgtatgtcc gggatgaccg gaatgcgggc tcatcagagc caccggcggt
    12061 ctggttcgcc tactcaccag acaggcaggg aaaacatccg gtacaacacc ttcgtccctt
    12121 ccggggtatc ctgcaggcgg atgcattcag cggttacgat cggctgttca gtgccaaacg
    12181 tgaaggtggt gcacagacag aagttgcgtg ctgggtccat gcccggcgaa aaatacacga
    12241 tgtatgaacg acatctcttc tgacgacat
//
LOCUS       DAPOZO010000128        12195 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPOZO010000128 DAPOZO010000000
VERSION     DAPOZO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.128"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(57..746)
                     /gene="pepE"
                     /locus_tag="ROI21_004146"
     CDS             complement(57..746)
                     /gene="pepE"
                     /locus_tag="ROI21_004146"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDX5113004.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLASLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(1128..2357)
                     /gene="sorE"
                     /locus_tag="ROI21_004147"
     CDS             complement(1128..2357)
                     /gene="sorE"
                     /locus_tag="ROI21_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HDX5113005.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            complement(2409..3233)
                     /locus_tag="ROI21_004148"
     CDS             complement(2409..3233)
                     /locus_tag="ROI21_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDX5113006.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            complement(3244..4041)
                     /locus_tag="ROI21_004149"
     CDS             complement(3244..4041)
                     /locus_tag="ROI21_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDX5113007.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            complement(4107..4601)
                     /locus_tag="ROI21_004150"
     CDS             complement(4107..4601)
                     /locus_tag="ROI21_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDX5113008.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(4601..5008)
                     /locus_tag="ROI21_004151"
     CDS             complement(4601..5008)
                     /locus_tag="ROI21_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HDX5113009.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDAHVEAVEFVPGENANDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMGNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQSCVVWRQVATAEEDF"
     gene            complement(5018..5824)
                     /locus_tag="ROI21_004152"
     CDS             complement(5018..5824)
                     /locus_tag="ROI21_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX5113010.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLIDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNSIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(5894..6841)
                     /locus_tag="ROI21_004153"
     CDS             complement(5894..6841)
                     /locus_tag="ROI21_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDX5113011.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFYDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            7189..8061
                     /gene="rluF"
                     /locus_tag="ROI21_004154"
     CDS             7189..8061
                     /gene="rluF"
                     /locus_tag="ROI21_004154"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDX5113012.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKSKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(8062..8334)
                     /locus_tag="ROI21_004155"
     CDS             complement(8062..8334)
                     /locus_tag="ROI21_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDX5113013.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(8587..9936)
                     /gene="lysC"
                     /locus_tag="ROI21_004156"
     CDS             complement(8587..9936)
                     /gene="lysC"
                     /locus_tag="ROI21_004156"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDX5113014.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            10461..12110
                     /gene="pgi"
                     /locus_tag="ROI21_004157"
     CDS             10461..12110
                     /gene="pgi"
                     /locus_tag="ROI21_004157"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDX5113015.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSQAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVNYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         3007 a         3216 c         3033 g         2939 t
ORIGIN      
        1 aagcgtcgca tcaggcacta tgtacaagat gccggatcat gcatccggca attcacttaa
       61 aaacggtgac ccgcttccag cggaaccgct tcttcaccag ccttaaacac ataagtggtg
      121 ttcgggccac ccagcgtagc gtgaccttta ctcaccgtga tccagttacc ttccggtaga
      181 ccaataattg tgagttctgg cgcgacgacc agcagttcgc gaatacgctg ctcacgcgtt
      241 tcacctttat ggccttccgg cagcgcgttg gtgaagtgcg ggttgatttg caacgggaac
      301 agattcagcg catcgaaacc ttgtggatcg acaatcggca tatcgttggt ggtacgaatg
      361 gttgggcaag caaggttagc gccagcgctc cagccaatat acagcgcgcc acgtttaacc
      421 acgtcagtaa ttggtgccag cagcccgcgc tcgcggcact gtttcagcaa ctggaaagta
      481 ttaccgccgc cgacaatcac gatctcagca ttttcaatcg cggcaacggg atcgacaacg
      541 ctatgaatac cggtgacaga aacacccagc gaagcgagaa ccgcagccgt tttcgctgtg
      601 taatcatccc aggtctgcgt tacgccagcg aaagggataa acaccgctga gcggcgaccc
      661 tgcaactgtt cagcaattag cggcagtgca tgttccagcc aggctttacc cggcagcgtc
      721 gagttactca ataaaagcag ttccattact tctccggtta gtaagaaatc acgggaggca
      781 tgctaccact gcgaatttaa ccacttactg acatggctca cgatgtcacg tattgcaccg
      841 ggcgctgaga ctgatgacaa acacagcatt gcctgatgcg ctacgcttac caggcctacc
      901 agataactgc aatatattga attttcgaga ttatgtatgc cgaataaggc ttcattccgc
      961 atccggcata aacaatgtgt actttgccac caatttgagc gccccttaag gcgctgatat
     1021 tttttatcct tctcatgcaa attgtgctgc ttcagcaatc agccgcacat ccggtcgcga
     1081 agttgccgcg gcgaccagcc agcgacatgc agagttaagt agcgcgatta atcatgggaa
     1141 attgcctctg catgagtgag cagatattgc tccgcctcac cggaccagat cccctgatga
     1201 cgcgccagaa ttgccgccag tgcttgatcc tgaatctgcg tgagtgacat aagcggcagg
     1261 tatttcccgg tatacaccag ctttttgccg ccgccgacgg caggcaattc aagcgtggtt
     1321 tcgcccgcgg cattcagccc aagaatatgt gttaccactt ttgcggcctg cacttttttc
     1381 tcttcaatca atttgactgc tgcgcgcatg tcatcggtat tgccgcctga cgtgcccacg
     1441 tagtgggtaa atgcataatg cacatcgtag aaattaattg gcgcgctgaa atgtttatcc
     1501 tgcggtccgg cgaagaaatt caggcaacca tctgtcgcca gcaaggaaga ggcgagagtc
     1561 accagtcctt cattaggcac aaagacgaaa atatcatcga agccgtgacc gccactcagt
     1621 gccattaacg tatcaaatgc tgcatcggca gcattgagat aatgaatcag tgtttgcggt
     1681 tctgatggat agtgcttgcg cgcataactc aatttgtcgt tgtcggtatc ggtaatgacg
     1741 agcagcgacg ggttaacggg tccatgtagc gcataatcaa tcgccaacag tcccattggt
     1801 ccggtgccgc cgaggatcag cgtgcgccct tgcgggcgaa tccccatcgt gtggttataa
     1861 ctaccttcct gaagatgata gttggcgttg aacgcgccaa tcacgcagga aagcggttca
     1921 accagcgagc cttcaaaata ggtttcgccg tcgtatgcca gcaggcaatc ttgttccatg
     1981 acctcgttgg gaataaccac atgcgtggct tcgccgccta cccacgggaa ggagtagccg
     2041 gggcagtccg ggcggtcggg gagttgcagg ttggcctgaa tcacataacg ctgacccggc
     2101 tggaatttgt gctgccactt tttacccacg gccagaatat cgccgcaaaa ctcgtggccg
     2161 atgatgatgg ggttggtcgc cacatcgtcg ggtacttttt tatggttttc acccagattg
     2221 gcctctttcc aggaagagag gcacaggctg tcggtgacca ccgtcgcgag gatttcatcc
     2281 tcctgcattt caggaagatc aaaggtttcc aggcgtaaat cacgtttacc ataaagacgc
     2341 agagctgttg ttttcataaa ccacctcatg ttgagcgatg gccccggcag tcgtgccggg
     2401 gcgaagaatc aggacaggaa gcccagcgca ttgccgataa tccctaagcc aaacagggcg
     2461 aaaatcagcc ataccgggtt aatttttttg ttgagcagac gaaccatcag tagcgtcaga
     2521 ccgagcgcca gcaaaccagg gcaaagttgg tcgagaatgt tctgcacggt catggtgacg
     2581 gtggagccat cggcggcatg cgtttgtgaa accaccaacg gtacgttgat tgacgtccat
     2641 ttggtcacca gcacgcccat cacaaacagt ccgagaatcg acgcgccttc ggtgagtttt
     2701 tgcagcacat tcccgcccat atcgctgacg atattcaccc ctttgcgaaa gccgagctgt
     2761 aggccatacc acttcatcgc cagacgcacc gcattgaaaa taaagaaaaa cagcagcggg
     2821 ccgagaatgt tgcccgaaag tgccagagat gcgccgagcg cggcggtaat cgggcgcagc
     2881 gttccccaga ccagtggatc gccaactcct gccaatggtc ccatcagacc gactttgatg
     2941 ccgttaatgg taccgtcatc aatttccgcg ccgttggccc gcgcctcttc catggcggca
     3001 gtgacgccga tgaccgggcc acatacggct ggcgtggtat tgaagaacac caggtgtcgc
     3061 ctgagcgccg caacctgatc ctcttttaat gggtaaagtc gcttgatggc ggggatcatg
     3121 tcgtagcaaa aacccagccc gtgaatacgt tcaaagttaa aggacgcctg ttgcaggttg
     3181 gagcgcagaa acatgctcac cagatcgctg cgtgtaattt ttctctgttc catgatggct
     3241 ccgttaatcg tcaagttggt caagggcggt ggaggcggtg gcctggagtt gaggttcagc
     3301 tttacgccac tgtggattta gctggatgta gagcagggcc atgatcacgc cgacgccacc
     3361 gaacgccagc agactgagat cgaggtagcc acctgcgagg aaaccgagga aaaagaaagg
     3421 catcaaatat ttcacgccca tcatacgtag caccatggcg taaccgacca ccacgataaa
     3481 accgccagca atctgtagtc ctcgggtcac aaattcggga atggcgttta acatattgct
     3541 gaccatatcg gcgctgacga acagcgagac aatcagtgcc ggaatagcaa cgcgcagcgc
     3601 ctgcacgcca agcgcggaga catgcagaat atcgagagtg cgaaaccgcg cctcttctgc
     3661 tgctttatcc gccgcgtgct ggaacactac tgtgatggta cgggcaaaca ccgtcagcac
     3721 ctggcctgcc gcagccacag gcaacgcgat ggcgattccg gtggcgatgc tttgctgacc
     3781 aacgataacc agaatggcgg agattatgct ggcgagtgca gaatctggag attgcgccgc
     3841 gccgacgttc atccagccga gagctatcaa ttccagcgta ccaccgagta ttattccggt
     3901 ttttaaatca ccgagaatta aaccaatcac cgtacaggca attaacgggc gatgagtctg
     3961 aaattcatcc agcacgctgc ccattccggc aatacaggaa aaaagaaata tagcaatgat
     4021 ttgtagggta cttatttcca tgatttgtca ccttttaagt gtcgtcatcc ctgcctgtta
     4081 aagcatatgt acacaggcgc tattatttaa tttgcgatta actgttcgtt tattttgtcg
     4141 ataatattga ttgagggatc tgacgctacg acgcgtaaat ccagtatgac gccaagatta
     4201 tttaactcat gaaatgcgtt aatatcatca tcatctaatg aaaccgcttt ggttaactgt
     4261 tttttaccgg gccgccaggc catgccgcca atattcaggg tgcctatttt taccccctgg
     4321 cgaaccatcg ctaaggcatc ctgtgggcga gtgaaaagat aaaaaacggt ttcgtcctga
     4381 tattgcgggt tgtgataaac cgcgacggct ttttcaatat tgaccacgtt aaccttcatt
     4441 cccggtggcg cagcctgacg caataatgtc cggcgaactt catcgttata aacttcgtca
     4501 ttgcagataa taatgcgctg ggcgttagcc actttcgacc agacggtagt gacctgaccg
     4561 tggatcaggc ggtcatcaat gcgggcgagc gtaatgttca tcagaaatcc tcctctgccg
     4621 ttgccacctg tctccagacg acgcaggatt gctttgcgat ttgcgtcagg tgttcacaca
     4681 gttcatcaac attcatgctg tcctggttat caaccagttc gagcgccagc ggcagagaaa
     4741 ggccgctgat tacccgcaga cgaggattgc ccatcgcaag catcgctgcc gcattccacg
     4801 ggctgccgca ctgtaagtcc acggcaatca gccattcgtc ttgattgtga atgctcacta
     4861 acttttccag ctttgccaca atgtcattgg cgttctcacc gggtacaaat tccactgcct
     4921 caacatgggc gtcgccgtaa accatctgaa ccgattccag catggcgcaa gctaatttgc
     4981 cgtgggcaca aaaaatggcg ttgaccataa agcctcctta gccgcgcgtt ttgccgcctg
     5041 caatgttagt ggttactccg gtgatatagc tggcgcgttc agacagcaga taacaaacaa
     5101 aatcagccac ttctgctaat cttccggcgc gcccaatagg aatggaattt ttggtatagc
     5161 cttcacgcag ctgctcgacg gtgatattgc gcgtccacgc cagcgcttct tcatattccg
     5221 gcgtgcgcag tcctgttttt tccagaatcc ctggcgcgat accgaccaca cggataccgt
     5281 gcttacccag ctctttcgac caggagcgcg tgaagctatt gagcgcggct ttggtcgcgg
     5341 cgtaacagct ttggccttct gagccttcca gcccactttc tgaggaaaca ttcacaatca
     5401 cgccatcatg ttgtttgacc atctgtcgcg ccaccgcctg cgacatcaga aaaacgcctt
     5461 tctggttgat attgaccatt ttttcgaatg cagcttcgtt gagttcatac tgcccggcag
     5521 gcgctttctc atcgatcagc agacgcggga aattgacccc ggcgttattg accagaccgt
     5581 cgatgcgacc aaaacgctgg ataatttccg ctaccgtatg atttacctct ttggcgctgg
     5641 aaatatcggt cggccaaaac tgatagcctt tatgaccttc atattgacca tcgccaccgt
     5701 gaatatcgac catctgtacg tttgcgcctt gtgctaataa ttcctctaca atcgccagac
     5761 caataccgga tgcgccgccg gtgacgataa tgattttatc ctgcaaattt aaccacgttt
     5821 gcataacaga ctcctgattt tagataatag ggacccgccg taatttttat tacggtaaac
     5881 atcgggaaga tttttatttc agtaatagtt cagctgtgct gctattcgtt accagacaat
     5941 taatatattt tccacgcagt gcaccaacaa ttccgctgta tttttcttca ctcatggcaa
     6001 tgccaatgga ataccgcgcc tgctttaatt tattcatttc gatagagagt gttttttcgc
     6061 tcatattcgt ttcaaccatt gcgccgtgaa tatcataaaa gcgcgagcaa atatcgccag
     6121 caacctggcg ggcattcagg tcgtcactct cttcaccacc ataaaacgca tgccagttag
     6181 cgccgtcgcg aatggccggt gagccaattc ccaccagggc gacatccaga ttatcccagt
     6241 aggcagagat ggttttaaag tgctgagact gcatgatccc attacgaatt aatgggttat
     6301 ccagcagagc cggaaaatcc gcgagatgcg attccccttt cagcttcgct gccgcgctgt
     6361 aggttaatgt gtttacgtga tagcggcttt cgagtttacc ggacggtccg ccaataattg
     6421 gtacgcagat taactgccgc gattgccccg cctgcggcaa gttttcaacc agtgcgctca
     6481 ccgcgcgacc ccaggaaaaa ccgacaatat cgccaggctc cagcaagcga tccagcagtt
     6541 gcgcgccgtg taaccccatc atcgccagtt gagtctcttc atcctcatca tttcccgaca
     6601 ccaccacaac gtctttcagg ccaaactttt gcttcagttg ctgctccagc cagagatttt
     6661 cgttgtagtc atagttgatg gcgatggtga caattccctg atcgcggcct cgtttaagca
     6721 agcggctgat ggtggtgcgg taaataccca gttcgcgcgc gatttgcgcc tgcgtcatat
     6781 cctgttcgta atagagttgg gcaatcttca caatcaaacg gatatcgtca ctgttttcca
     6841 ttgccgcctc ctggttctgc acatttgtgc aaatacgctt tctcaccgct tgatctgata
     6901 taaccccatc atgatggcct ttcaaagcga tttttcactc tttgcatggg gagaaatagc
     6961 gctttctctt gcacatttgt ttctctgcct agtagtcatc agcacagttg tgcagcgatt
     7021 cgtaatatgt tgattatcct tattttgcca ggcaaatttg catcgtactt ccggagacac
     7081 ttttgtgata aaaggcgtgg cagatcacat ttaatttcga cgttgccgca taaccctgtt
     7141 tttccccgct aagaggtacg gtatattccg cgttttacag gagaatttat gctgcccgac
     7201 tcatcagtcc gtttaaataa atacatcagc gaaagcggaa tttgctcacg ccgcgaagcg
     7261 gatcgctata tcgagcaagg caatgtgttc cttaatggca agcgagccac cattggcgat
     7321 caggtgaaac ccggcgacgt tgtgaaagta aacggtcagt tgattgagcc tcgggaagcc
     7381 gaagatttgg tacttatcgc cctgaacaag cccgtcggta ttgtcagcac caccgaagat
     7441 ggcgagcgcg ataacattgt cgatttcgtt aaccacagca aacgcgtgtt cccgattggc
     7501 cgcctggata aagactccca ggggctgatt ttcctcacca atcacggcga tctggtgaat
     7561 aagatcctcc gtgcgggtaa cgatcatgag aaagagtatc tggtgacggt cgataaaccg
     7621 attaccgatg aatttattcg tggtatgggg gcgggggtgc cgatcctcgg gacggtgacg
     7681 aaaaagtgca aagtcaaaaa agaagcgccg tttgtcttcc gcattaccct ggtgcagggg
     7741 ctgaaccgtc agatccgtcg catgtgcgag cactttggtt acgaagtgaa aaagctggaa
     7801 cgtacgcgca tcatgaacgt tagtttaagc ggcattccgc tgggggaatg gcgtgattta
     7861 actgacgatg agttaatcga cctctttaag ctcattgaaa attcctcttc cgaggcaaaa
     7921 tctaaagcga aggccaaacc gaaaactgta ggcattaaac gtccagtcgt taagatggaa
     7981 aaaacggcgg aaaaaggcgg tcgcccggcg tccaacggta agcgttttac ctcgccgggg
     8041 cgtaaaaaga aggggcgcta atcagcgcct gccgcgcgtc gacgtatttg ctgaccagct
     8101 aaatgacgct tcaggatccg gtttataagc ctgctttttc tttaactggc gggctttttt
     8161 cgcttcagcc tcacgctcga ccattaattt atcgatgtac tctttcttaa tgctgttggt
     8221 ttcggagttg gtcagtacgc gaccatgcgc aatccgcgcc cgatccagca gtgttttaag
     8281 ttcacgctgc tcgcgttcag tcatctcttt ttgggtaata cgggggagtg ccatgctggt
     8341 gccctcagtg agccagagag ttcagtgtac gggaattcgt ttgcgagcag aactgataat
     8401 attcattttg ccttctctta actatttatc tctttcgata attaattaga gatgcagata
     8461 aaaaaattaa aggcaattat tctccgataa agacgttggt taacagagta caggctcgtt
     8521 tattgatgag catagtgaca agaaaatcaa tacggcccga aatatagctt ccaggccata
     8581 cagtatttac tcaaacaaat tactatgcag tttttgcacc acctgctcgg catcttcgcc
     8641 gggcaccagg aagcacaggt tatggctgga tgcgccgtaa caaatcatgc gaatgttgaa
     8701 cggttccagt acgccgaata cctctttgcc aacgccgcag gcttttgaca ggtcattgcc
     8761 aatcaacgcg accagcgcca gaccttcctc cacttctacc cggcatagtg cagacagctc
     8821 catcagcaga gattgcgtca gcaaggtatc gccagtggag gttgaaccgg tggtatcaag
     8881 ggttaatgcc acgctcactt ctgacgtggt gattaagtct accgaaatat tatgccgcgc
     8941 gaggatgccg aaaacttccg cgaggaaacc gcgagaatgc agcatattca ggctgtgcaa
     9001 agtgagcaga gtctgattgc gacgaagcgc cagcgcgcgg aatagcggcg gattttcagt
     9061 tttattgcac accagcgtac cacctgcgcg tgggtcttta ctggagccga caaagaccgg
     9121 gatatcgctg cgtactgcgg gtagcaacgt tgccggatgc agtacttttg caccaaaagt
     9181 tgccatctct gccgcttcgg caaacgcgat ttcatcaatg cgttttgctg cggaaaccac
     9241 gcgtggatcg gtggtgtaga tgcccgggac gtcggtccag atatcaacac gagatgcgtg
     9301 taaagcctcc gccagcaagg ctgccgtata atcgctgcct ccacggccaa gcgtcgttgt
     9361 ccgaccttta ttttcgctac cgataaatcc ctgggtgatc actaagcctt cattgaggcg
     9421 tgggagcagc tgcagcgcgg ccagttccgc cagcgcggct acatctggct ctgcacgacc
     9481 aaatcggtcg ttggtacgca tcactttacg tacatcaaac cactgtgcct gaacatcgcg
     9541 ttcgcgcagg atttcgacaa acagcagggt cgacatcagc tcgccgtggc tgaccagttc
     9601 atctgtcagc gccggagacg ttgccagcgc cgccgcttct gccagaacag taatgttctc
     9661 cagcagacgt tcaatctctt cacggataac gttcgggtaa cgcagacgtt ccagaatggc
     9721 aaactggata ttgcggattg cgtcgagttt ttcgaatcgc tcgccaggtt ccagtccttc
     9781 agctaaagcg accagcagat tagtgatacc agcagaagcc gagaggacaa ctaaacgtac
     9841 gttggcatca gaaagcacaa tatcagcgct gcggttcatg gcgtcaaaat cagctacgct
     9901 ggtaccgcca aatttggaga caacaatttc agacataact acctcgtgtc aggggatcca
     9961 ttttcagcct tggcacaagg gaagagcgga agacgggtgg gcgcagagcg atacttcgct
    10021 actattttca cccagaagtg ctccaccact tgcgaaacgc ccgactgcga acgcttctgg
    10081 tgacaaccca ggggattcag cccctgtagc cgatgatgaa cgtggccagc cgttcaatca
    10141 cctcggcgat gcaccccctc aggtgttatc acaggactgg ctcctccaac accgttactt
    10201 gggcaacgcg cctcttctgg cctgcgctag cgcaggtagt acatttataa ataaagggtg
    10261 agtggggcgg ttgtcaacga tggggtcatg cggatttttc atccactcct ggcggtcagt
    10321 agttcagcta ataaatgctt cactgcgcta agggtttaca ctcaacatta cgctaacggc
    10381 actaaaacca tcacattttt ctgtgactgg cgctacaatc ttccaaagtc acaattctca
    10441 aaatcagaag agtattgcta atgaaaaaca tcaatccaac gcagaccgct gcctggcagg
    10501 cactacagaa acacttcgat gaaatgaaag acgttacgat cgccgatctt tttgctaaag
    10561 acggcgatcg tttttctaag ttctccgcaa ccttcgacga tcagatgctg gtggattact
    10621 ccaaaaaccg catcactgaa gagacgctgg cgaaattaca agatctggcg aaagagtgcg
    10681 atctggcggg cgcgattaag tcgatgttct ctggtgagaa gatcaaccgc accgaaaacc
    10741 gcgccgtgct gcacgtagcg ctgcgtaacc gtagcaatac cccgattctg gttgatggca
    10801 aagacgttat gccggaagtc aacgcggtgc tggagaagat gaaaaccttc tcgcaagcga
    10861 ttatttccgg tgagtggaag ggttataccg gcaaagcaat cactgacgta gtgaacatcg
    10921 ggatcggcgg ttctgacctc ggcccataca tggtgaccga agcactgcgt ccgtacaaaa
    10981 accacctgaa catgcacttt gtttctaacg tcgatgggac tcacatcgcg gaagtgctga
    11041 aaaaagtaaa cccggaaacc acgctgttcc tggtagcatc taaaaccttc accactcagg
    11101 aaaccatgac caacgcccat agtgcgcgtg actggttcct gaaagcggct ggtgatgaaa
    11161 aacacgttgc gaaacacttt gcggcgctct caaccaacgc caaagccgtt ggcgagtttg
    11221 gtattgatac cgccaacatg ttcgagttct gggactgggt cggtggtcgt tactctttat
    11281 ggtcagcgat tggcctgtcg attgttctct ccatcggctt tgataacttc gttgaactgc
    11341 tttctggcgc acacgcgatg gacaagcatt tctccaccac gcctgccgag aaaaacctgc
    11401 ctgtactact ggcgctgatt ggcatctggt acaacaattt ctttggcgcg gaaactgaag
    11461 cgattctgcc gtatgaccag tatatgcacc gtttcgcggc gtacttccag cagggcaata
    11521 tggagtccaa cggtaagtac gttgaccgta acggtaacgt cgtgaattac cagactggcc
    11581 cgattatctg gggtgaacca ggcactaacg gtcagcacgc gttctaccag ctgatccacc
    11641 agggaaccaa aatggtaccg tgcgatttca tcgccccggc tatcacccat aacccgctct
    11701 ctgatcatca ccagaaactg ctgtctaact tctttgctca gaccgaagcg ctggcgtttg
    11761 gtaaatcccg cgaagtggtt gagcaggaat atcgtgatca gggtaaagat ccggcaacgc
    11821 ttgactacgt ggtgccgttc aaagtgttcg aaggtaaccg cccgaccaac tccatcctgc
    11881 tgcgcgaaat cactccgttc agcctgggtg cgttgattgc gctgtatgag cacaaaatct
    11941 ttactcaggg cgtgattctg aacatcttca ccttcgacca gtggggcgtg gagctgggta
    12001 aacaactggc gaaccgtatt ctgccagagc tgaaagatga taaagaaatc agcagccacg
    12061 atagctcgac caatggtctg attaaccgct ataaagcgtg gcgcggttaa ttaaataacc
    12121 ttaataaaaa tgccgacgtt atgtcggcat tttttatcag ataaatcccc ttgtctgtga
    12181 tttaacggaa atcat
//
LOCUS       DAPOZO010000129        12075 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPOZO010000129 DAPOZO010000000
VERSION     DAPOZO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.129"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(5..1009)
                     /gene="oppF"
                     /locus_tag="ROI21_004158"
     CDS             complement(5..1009)
                     /gene="oppF"
                     /locus_tag="ROI21_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDX5113016.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(1006..2019)
                     /gene="oppD"
                     /locus_tag="ROI21_004159"
     CDS             complement(1006..2019)
                     /gene="oppD"
                     /locus_tag="ROI21_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDX5113017.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(2031..2939)
                     /gene="oppC"
                     /locus_tag="ROI21_004160"
     CDS             complement(2031..2939)
                     /gene="oppC"
                     /locus_tag="ROI21_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDX5113018.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(2954..3874)
                     /gene="oppB"
                     /locus_tag="ROI21_004161"
     CDS             complement(2954..3874)
                     /gene="oppB"
                     /locus_tag="ROI21_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDX5113019.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(3960..5591)
                     /gene="oppA"
                     /locus_tag="ROI21_004162"
     CDS             complement(3960..5591)
                     /gene="oppA"
                     /locus_tag="ROI21_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDX5113020.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTVDKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            5875..6171
                     /locus_tag="ROI21_004163"
     CDS             5875..6171
                     /locus_tag="ROI21_004163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113021.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(6329..6976)
                     /gene="ychE"
                     /locus_tag="ROI21_004164"
     CDS             complement(6329..6976)
                     /gene="ychE"
                     /locus_tag="ROI21_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDX5113022.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            7453..10128
                     /gene="adhE"
                     /locus_tag="ROI21_004165"
                     /gene_synonym="adhC"
     CDS             7453..10128
                     /gene="adhE"
                     /locus_tag="ROI21_004165"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDX5113023.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(10430..11047)
                     /gene="tdk"
                     /locus_tag="ROI21_004166"
     CDS             complement(10430..11047)
                     /gene="tdk"
                     /locus_tag="ROI21_004166"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDX5113024.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            11651..12064
                     /gene="hns"
                     /locus_tag="ROI21_004167"
     CDS             11651..12064
                     /gene="hns"
                     /locus_tag="ROI21_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDX5113025.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
BASE COUNT         3299 a         3027 c         2734 g         3015 t
ORIGIN      
        1 gtgtttaaag cggatcgact ttcaggcaag aaacggcgtg tctgaaactc ccctccagaa
       61 caggacgtgt tttggcgcac tccggaccgg caatcgggca acgggtacgg aaaacacaac
      121 cggaaggcgg gttgatcggc gacggtaatt ccccttccag taactggatg gttttgttct
      181 tctccagatc cggatcaggt atggggactg ccgacatcaa tgccctggtg taaggatgta
      241 gtggattgtg gtagacctca tcataggtcc ccagttctac cgcatggccg agatacatca
      301 ccagcacacg atcggaaatg tgttttacca cggccaggtc gtgagcgata aaaattaatg
      361 acaatcccat ctcgcgttgc agctgctgga gcaggttgac cacctgcgcc tgaattgaca
      421 cgtccagcgc cgacaccggc tcatcacaga taatcagctt cggttcaaga ataagagcac
      481 gagcaatccc gatacgctgg cactgcccac cagagaactc atgcggatag cggttaatca
      541 ggttaggcaa taacccgact ttcagcatca tcgccttcac gcgctcgcga acttcctggc
      601 gtgacatttt cggatgataa gtacgcagtg gttcagcgat gatctcgccg atggtcatac
      661 gcgggttaag cgatgccaac ggatcctgga aaatcatctg aatatcactg cgaacggcac
      721 gccattcatc gggcttcatg cccagcaact ctttacctaa ccaggcaaca tgaccgtcgg
      781 tcgccttgac caaaccgatg atggcgcgag caaaggtgga cttaccgcat cccgattccc
      841 ctaccacacc taaagtttcc ccttcataca ggcgaagagt gacaccatcg acggctttga
      901 gcgttttcgg cggttgccag aaccactgtt tgccatcttt gatttcaaag tgcactttca
      961 gatcggcgat ttcaaggagg acttttcttc cttcagttac agcattcata acagttcctc
     1021 caccggttta aagcaagcac gcagacggcc aggcgtaaac tcttccagcg gcggtgcgct
     1081 actacaaatt tccatcgcat gcggacaacg tggctggaac gggcaacctt tcggtaatcg
     1141 cagcaagttt ggcggattac ccgggatggt caacattgtt tcaccttccg catcaagacg
     1201 cggcaccgcg ttgagcaaac cgatagaata aggatgaacg ggttgataaa agacatcgcg
     1261 cgcattgcca tattccatcg tgcgcccggc gtacattacc agcactttgt cgcagatccc
     1321 cgcaaccacg ccaagatcgt gggtgatcat aatgatggcg gtattaaatt cccgcttcag
     1381 ttcatttaat agcgtcatga tctgcgcctg tacggtgacg tccagcgcag tagtgggttc
     1441 atcagcaatc agcagcttag gtcgacatag caatgccatc gcaatcatga ctcgctgacg
     1501 catgccgcca gaaaattcgt gcgggtacat tttcatgcgt ttacgcgctt ccggcatttt
     1561 taccgcatcg agcatccgca ccgactcttc aaacgcctca gctttgctca tgttcttatg
     1621 cagcatcagc acttccatca actgctcacc gacgcgcata tagggattca acgaagtcat
     1681 tgggtcctga aaaatcattg agatttgttc agcgcgcagt ttattgagtt cacgctctgg
     1741 caaattgagg atttcacgcc cattgaaggt tgccgatccg ccaatacgtc cgttggcagc
     1801 caacaggccc atcaacgcaa atgcagtttg cgatttaccc gaaccagact cacctacaat
     1861 gcccagcgtt tctccggcac gtagggaaaa atttaaatca ttgaccgccg tgacgtcgcc
     1921 gtccggggta ctaaaggtaa cacgcaaatc tttcacgttc agcagtgcgt cagcctgttg
     1981 ttgtgcgagc ggcacagttg cagtttcaat tacgctcatg gctgcactcc ttaacgatct
     2041 ttcgggtcga gggcatcacg caagccatcg ccgataaagt tgaaacaaaa cagtgtcacc
     2101 acgaggaatc ccgctgggaa caacaataac catggagaga cttccatcga gttcgcgcca
     2161 tcactcagca atgcacccca gctgcttaac ggctcttgcg tacccaaccc caggaagcta
     2221 aggaaagatt caaagaggat catgctgggc accagaagtg atgcgtagac caccaccaca
     2281 ccgagtacat tcggcacaat gtggcgaata acaatgcccg acgtcgatac accgccaact
     2341 tgtgccgcct caataaactc tttacgcttc agactcaggg tttgcccacg cacaatacga
     2401 gccatatcca gccaggaaac catgccaatc gccacgaaaa tcagcaggat gttttgaccg
     2461 aaaaaggtca ccagcaaaat gacgaagaac atgaatggga aggagttgag gatttccagc
     2521 agacgcatca ttaccgaatc cactttaccg cccagataac cggaaagcga accgtaaagt
     2581 gtccccacga ccactgccac cagtgccgca gcaacaccga ccatgagtga gatacgcccg
     2641 ccaatcgcaa cgcgcacaag caggtcgcga ccggatgagt cagtaccaaa gtagtgaccg
     2701 gactccatat ccggggcgct ggacatcatc gcccagtcag tatcgtcata ggcaaactgc
     2761 gaaagcatcg gtgccaggat tacaaataac gcgatcagca ccagcactat cagactggca
     2821 accgccgcac ggttatgcat aaaacgtcga cgtgcgtcct gccacaagct gcgcccttcg
     2881 acctccagct tttcactgaa attttccagc gtctcgctgt ttttcttact taacatcatc
     2941 gcgagctcca gtatcagtaa cggattttcg ggtcgataac cgcatatagc acatcgacaa
     3001 tggcattaaa caaaatggtt aacgcaccaa ccaggatagt caggcttaac actaaggaat
     3061 agtcacggtt caatgcgcca ttaacgaaca gttgcccaat ccccggcaaa ccataaatgg
     3121 tttcgataac catagaaccg gtaataatgc cgacaaatgc ggggcccata taggagagca
     3181 cgggtaacag agcaggtttt aatgcgtggc gtaaaatgat ccgccgcata ggtaaccctt
     3241 tcgcccgggc agtacgaata aagttggagt gtaaaacctc aatcatagag ccacgggtaa
     3301 tacgcgcaat actggcgata taggcgagtg acaacgccac catcggcaat atcatgaatt
     3361 taagcgcccc accattccag ccaccgcccg gcagccaatg caaaatgatc gcaaatatca
     3421 ttactaataa tggcgcaacc acaaaactgg ggataacaac cccggtcatt gccagcccca
     3481 tcacggtata gtcccatttg gtgttttgtt ttaatgcggc aataacgcca gcactaacac
     3541 ccagtattac cgcaaggaaa aatgctgcgg ctcccagttt ggcagaaacg ggaaaactgg
     3601 atgcaaccag gtcgttgacc gaataatctt tatatttaaa cgatggaccg aaatcgccat
     3661 gcgccagttg tttcaggtag ctgaaatact gtgtcatgat cggatcatta agatgatatt
     3721 tcgcttcgat attggccatc acttctggcg gtaaagtacg ttcgccggta aaagggcttc
     3781 ccggcgcgag gcgcatcatg aagaacgaaa tagtaataag aataaatagc gtcggaatcg
     3841 cttccagaca gcgacgtaga ataaatttta acattgccct accttctggc ctgtgccttc
     3901 tgtaatgcga taaaaatcag acaccgtgga gcaggacact cctgccccac gtattgccat
     3961 tagtgcttca caatgtacat attccgggtg taggtattat ccagcggatc tttgccggta
     4021 tagccaccaa cccacggttt caccagacgc gcattcacgt agtaataaac aggaacaatg
     4081 gccgaatcct tatccagctg ttgttctgct ttagtgtaca gagctgtgcg ctgcgcctcg
     4141 tcagtcgctt tcagcgtttc cgccataatg ctgtcaaagg ccgggctctt ataatgcgcg
     4201 gtattcatcg agctgttcga cagcatggtg ttcaggaagg aagttggttc gttgtagtct
     4261 gcacaccagc ctgcacgggc cacatcaaaa gtgccctggt gacgggtgtc gaggaacgtt
     4321 ttccactcct ggttaaccag tttgacgttt acaccaatgt ttttcttcca caatgaagag
     4381 gcagcaatcg ccagcttttt atgcagatcg gaggtgttat acaacaggtt gatggtcaac
     4441 ggtttgtcta cggtataacc cgcttcagcc agcagttttt tcgcttcttc gttacgtttt
     4501 tcctggctcc agccaaacca ttccggctga gtcaattttg cgccatcagt atacggtgga
     4561 gtgtaaccat aggcgggcat gtcgccctgc gctttcactt tattaacaat gatatcgcgg
     4621 tccataccca gtttcagtgc ggtacgcaca cgtacatcgt taaatggcgg tttctggttg
     4681 ttaatttcgt aataataagt gcacaggtat ggatcaacgt gaacttcgtc cgggatctct
     4741 tttttcagct tctggaacaa ttcgatcggc atgttgttat aagtcatgtc gatttcacca
     4801 ctacggtagc gattgacatc ggtaacttca gaagcaatag gcaaataggt tacctggtta
     4861 ataacggttt tcgcgttgtt ccagtaggtc gggctgcgtt caagaacgat tcgttcgttt
     4921 acgacccaat cttttaaggt ataggcaccg ttggtgacga tattaccagg ctgggtccat
     4981 ttttctccga atttctcgat agcggctttt ggcaccggtg aagttgatgg gtgaacaagt
     5041 aatttataga agtacggaac aggttcactt aaggtgactt ctaatgtgtg atcatcaata
     5101 gctttcacgc cgagatcggt aatcggtttt ttcccttcaa gaatttcatc aataccggcg
     5161 atatgcccat attgcagata actggcatac ggagaagcgg tgttcggatc aacagaacgt
     5221 tgccagctat acacaaagtc ttgggctgtg actggcgtgc catcagacca tttcgcatct
     5281 ttacgcaaat ggaaggtcca gactttcgcg tctttattat cccaggattc agcgacgcca
     5341 ggtgccggat ggccgtcaag atcgctgacc agtaagcctt caaacaggtc tcggctgata
     5401 ttagactccg gaacaccttc aattttgtgc ggatctaatg actgaacttc tgaaccattg
     5461 ttacgtacca gcgtttgttt ttccgccagt gtgacgcctg cgggtacatc agctgccagc
     5521 gcgacattcc ctgccattag cgcagccaga acgccagctg ctactaaact tctcttggtg
     5581 atgttggtca ttgttttttg gactccctca ttataattac tggtattaac cagcatgtgt
     5641 aatcccggat tgggaccctg cacaggctcg gagactttct gctgtcaggt tactttatcg
     5701 ttacgtatta ctatcaccga ctttatttat tctggtgatt cagacgatca ccttatgtcg
     5761 attcgtactg tgtctcccct gtcgagacac attattcttt cgcgacgctc tttaattgtt
     5821 aattattctc attaccaata aagaatcgtc tggcggttgc ccggaaagta ccaaatgcgc
     5881 ttcttactcg ccaatacatt ttgcaaaatt gttaagcaat tctcttttat tgaccgtatc
     5941 agaatctata gcttgcatcc atgtcttaac aaacatcttt caattcaatc aaatagagat
     6001 tggttaagca tgtctatctt ttctgattac agttccagta gtgaaatgca caataattta
     6061 acaattgatt attatttagc actttcctct accaaaggca gtggaattac taatatcatt
     6121 tcaattattc ttcagcaagc ccaggattat gatgtggcga aaataacatg atggtgagga
     6181 gaagcagtta aggaaatcct tataaccaac tgtaattcaa gaagaatatt tttacccatt
     6241 ccctttgctt ttgataactg caccggaaca aagtaaaaat aatgacgaag taaacggagc
     6301 atgacagctc cgtttcattt gaaaggaatt aattaagcaa gccggggaaa atccccttaa
     6361 taccagtaac gataaattca atccccaatg ccatcagcaa tagccccata atacgcgtga
     6421 tcacgttgat gccggtttgg cgtaaaaccc gtaccagcca cggtgccatg cggaacaatc
     6481 cccagcaaca taaagcgaac aatgcaatag ccacaaagaa accaaacaga tagctaatgc
     6541 tgtgataacg cgtaccccag acgatggtag aactgatcgc ccctggcccc gccatcaacg
     6601 gcaacgccag tggcaccaca ccaatgcttt cacgtaccgc ggtttctgat ttttcttgct
     6661 tgttctgttt atcctcgcca agcttgccgc tgatcatcga catcgctatc gtcaccacca
     6721 ggatgccccc ggcgatacgg aacgaatcaa ttgatatacc aaaaagttgc agaatcgtgt
     6781 cgccgagaaa aagcgagatc cacaagataa tggccacaga caggttggct gtaaggttag
     6841 ttttgtttcg cgctgccgct gtctgataac tggtcatgct gataaagacg ggaataatcc
     6901 ctaccgggtt gaccagcgca aataacccga tgaaaaattt gaagtaaacg ggaaaatcaa
     6961 aaagggtctg aatcacggtt agctccgaag caaaagccgg ataatgttag ccataaataa
     7021 ggttgaaaag acgcgctgac aatacgccct ttgacagcat ttttcacctc ctaactactt
     7081 aaaattgcta tcattcgtta ttgttatctg gttgtgcaaa acatgctaat gtagccacca
     7141 aatcatacta caatttatta actgttagct ataatggcga aaagcgatgc tgaaaggtgt
     7201 cagctttgca aaaatttgat ttggatcacg taatcagtac ccagaagtga gtaatcttgc
     7261 ttacgccacc tggaagtgac gcattagaga taataactct aatgtttaaa ctcttttagt
     7321 aaatcacagt gagtgtgagc gcgagtaagc ttttgatttt catcggttaa gcaaatcatc
     7381 accgcactga ctatactctc gtattcgagc agatgattta ctaaaaaagt ttaacattat
     7441 caggagagca ttatggctgt tactaatgtc gctgaactta acgcactcgt agagcgtgta
     7501 aaaaaagccc agcgtgaata tgccagtttc actcaagagc aagtagacaa aatcttccgc
     7561 gccgccgctc tggctgccgc agatgctcga atcccactcg cgaaaatggc cgttgccgaa
     7621 tccggcatgg gtatcgtcga agataaagtg atcaaaaacc actttgcttc tgaatatatc
     7681 tacaacgcct ataaagatga aaaaacctgt ggtgttctgt ctgaagacga cacttttggt
     7741 accatcacta tcgctgaacc aatcggtatt atttgcggta tcgttccgac cactaacccg
     7801 acttcaactg ctatcttcaa atcgctgatc agcctgaaga cccgtaacgc cattatcttc
     7861 tccccgcacc cacgtgcaaa agatgcaacc aacaaagcag ctgatatcgt tctgcaggct
     7921 gctatcgctg ccggtgctcc gaaagatctg atcggctgga tcgatcaacc ttcggttgaa
     7981 ctgtctaacg cactgatgca ccacccagac atcaacctga tcctcgcgac tggtggtccg
     8041 ggcatggtta aagccgcata cagctccggt aaaccagcta tcggtgtagg cgcgggtaac
     8101 acgcctgttg ttatcgatga aactgctgat atcaaacgtg cagttgcatc tgtgctgatg
     8161 tccaaaacct tcgacaacgg cgtaatctgt gcttctgaac agtctgttgt tgttgttgac
     8221 tctgtttatg acgcagtacg tgaacgtttc gcaacccatg gcggctatct gttgcagggt
     8281 aaagagctga aagctgttca ggacgttatc ctgaaaaacg gtgcgctgaa cgcggctatc
     8341 gttggtcagc cagcctataa aattgctgaa ctggcaggct tctctgtacc agaaaacacc
     8401 aagattctga tcggtgaagt gaccgttgtt gatgaaagcg aaccgttcgc acatgaaaaa
     8461 ctgtccccga ctctggcaat gtaccgcgct aaagatttcg aagacgcggt agaaaaagca
     8521 gagaaactgg tagctatggg cggtatcggt catacctctt gcctgtacac tgaccaggat
     8581 aaccaaccgg ctcgcgtttc ttacttcggt cagaaaatga aaacggctcg tatcctgatt
     8641 aacaccccgg cttctcaggg tggtatcggt gacctgtata acttcaaact cgcaccttcc
     8701 ctgactctgg gttgtggttc ctggggtggt aactccatct ctgaaaacgt tggtccgaaa
     8761 cacctgatca acaagaaaac cgttgctaag cgagctgaaa acatgttgtg gcacaaactt
     8821 ccgaaatcta tctacttccg ccgtggctcc ctgccaatcg cgctggatga agtgattact
     8881 gatggccaca aacgtgcgct catcgtgact gaccgcttcc tgttcaacaa tggttatgct
     8941 gatcagatca cttccgtact gaaagcagca ggcgttgaaa ctgaagtctt cttcgaagta
     9001 gaagctgacc cgaccctgag catcgttcgt aaaggtgcag aactggcaaa ctccttcaaa
     9061 ccagacgtga ttatcgcgct gggtggtggt tccccgatgg atgccgcgaa gatcatgtgg
     9121 gtcatgtacg aacatccgga aactcacttc gaagaactgg cgctgcgctt tatggatatc
     9181 cgtaaacgta tctacaagtt cccgaaaatg ggcgtgaaag cgaaaatgat cgctgtcacc
     9241 accacttctg gtacaggttc tgaagtcact ccgtttgcgg ttgtaactga cgacgctact
     9301 ggtcagaaat atccgctggc agactatgcg ctgaccccgg atatggcgat tgtcgatgcc
     9361 aacctggtta tggacatgcc gaagtccctg tgtgctttcg gtggtctgga cgcagtaact
     9421 cacgccatgg aagcttatgt ttctgtactg gcatctgagt tctctgatgg tcaggctctg
     9481 caggcgctga aactgctgaa agaatatctg ccagcgtcct accacgaagg atctaaaaat
     9541 ccggtagcgc gtgaacgtgt tcacagtgca gcaactatcg cgggtatcgc gtttgcgaac
     9601 gccttcctgg gtgtatgtca ctcaatggcg cacaaactgg gttcccagtt ccatattccg
     9661 cacggtctgg caaacgccct gctgatttgt aacgttattc gctacaacgc gaacgacaac
     9721 ccgaccaagc agactgcatt cagccagtat gaccgtccgc aagctcgccg tcgttatgct
     9781 gaaattgctg accacctggg tctgagcgca ccgggcgacc gtactgctgc taagatcgag
     9841 aaactgctgg catggctgga aacgctgaaa gctgaactgg gtattccgaa atctatccgt
     9901 gaagctggcg ttcaggaagc agacttcctg gcgaatgtgg ataaactgtc tgaagatgca
     9961 ttcgatgacc agtgcaccgg cgctaacccg cgttacccac tgatctccga gctgaaacag
    10021 attctgctgg atacctacta cggtcgtgat tatgtagaag gtgaaactgc agcgaaaaaa
    10081 gaagccgctc cggctaaagc tgagaaaaaa gcgaagaaat ccgcttaatc agtagcgctg
    10141 tctggcaaca taaacggtcc cttctggggc cgttttttgt tttacccaaa gcaactttcc
    10201 cataaaccga cagcatttgc cttcatcata tttacggcga tgtataacgc ctacacacag
    10261 cgatattgta ctttacaggt cacaagtcaa cgtcactgta aaagcgctct gtgaggggta
    10321 tagtggcgtt aaagaactgt cgagaagggt atatacaccg gatgaagtga gtaaaacgaa
    10381 ctgacaggat aaaagcgccc tgctcaccct gtcagtaaag aaattcttat taatcgtggc
    10441 gatgcctttc ctgaatagcc gttaatgagc cgacttctaa cgcttcttta tagtgtttac
    10501 ggcatacaga aacgtagcgt tcattaccac caataaccac ctgctcacct tcgttataag
    10561 gtctgcctgc ttgatcaaga cgcagcacca tgcttgcttt gcggccacaa aaacagatgg
    10621 tttttaattc aaccagtttg tcggaccatg ccagtaagta ttggctgcca ataaataact
    10681 cacctcgaaa atcggtacgt aaaccataac aaagtacggg tatatcgagt tgatcgacaa
    10741 cctccgataa ttcatatact tgttgtctgg ttaaaaactg gcattcatca accagtacgc
    10801 aatgaattgc ctgctgttca tgttccgcac gaatctcatc aaataatgat gaattttggt
    10861 taaataattt tgcaggcgat gacaaaccta tacgcgaact gactttcccg gcaccaaagc
    10921 gatcgtcaat ttctgccgta tatacgacag tgcgcatgcc gcgttcctgg taattgtatg
    10981 aagattgcaa caatgctgta gacttacccg cattcattgc ggaatagtag aaatatagct
    11041 gtgccatcag ccacaggccc tcaatgatat gaattaaagt tggctggagt ttatcataat
    11101 tcgtcagttt ttcagctcaa cagtatgcac agaagtggta tatgcgttct cccttacgaa
    11161 gccttgcata atccttctga gctatcatta caactgcctc gcttgttata agcgggtaaa
    11221 tgactgctgg taactattca caatctttaa cccgttgcgc aagtaatagc cctctgttga
    11281 cctccaggag atagtgcaat actaagtccc tgctcttatt gcgactgttc tacttttcat
    11341 cattcgctta atagggaatt ctcgtaaaca cgactaatac agaagactga aaggtcgtca
    11401 gcctacgata atctccccat aaaatgtgac atgaatcagg aagttttaac ctcacgtgct
    11461 gcgaaatcat cggcgcaaat agggctatat gccgcgtctt ttctggctaa ttttatgaaa
    11521 agatatttat tggaggcaca aaataaagaa caattttgaa ttccttacat tcctggctat
    11581 tgcacaactg aatttaaggc tctattatta cctcaacaaa ccaccccaat ataagtttga
    11641 gattactaca atgagcgaag cacttaaaat tctgaacaac atccgtactc ttcgtgcgca
    11701 ggcaagagaa tgtacacttg aaacgctgga agaaatgctg gaaaaattag aagttgttgt
    11761 taacgaacgt cgcgaagaag aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa
    11821 actgcagcaa tatcgcgaaa tgctgatcgc tgacggtatt gacccgaacg aactgctgaa
    11881 tagccttgct gccgttaaat ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata
    11941 tagctacgtt gacgaaaacg gcgaaactaa aacctggact ggccagggcc gtactccagc
    12001 tgtaatcaaa aaagcaatgg atgagcaagg taaatccctc gacgatttcc tgatcaagca
    12061 ataatctttt gtaga
//
LOCUS       DAPOZO010000130        12016 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPOZO010000130 DAPOZO010000000
VERSION     DAPOZO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.130"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..222
                     /locus_tag="ROI21_004168"
     CDS             <1..222
                     /locus_tag="ROI21_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113026.1"
                     /translation="KILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVMCDEN
                     YEGEKCCPSCGTGVYSREGGQEGNWKDRN"
     gene            506..2125
                     /locus_tag="ROI21_004169"
                     /pseudo
     CDS             506..2125
                     /locus_tag="ROI21_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2205..2497
                     /locus_tag="ROI21_004170"
                     /pseudo
     CDS             2205..2497
                     /locus_tag="ROI21_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(2537..3571)
                     /locus_tag="ROI21_004171"
     CDS             complement(2537..3571)
                     /locus_tag="ROI21_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HDX5113027.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQQ
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(3574..4539)
                     /locus_tag="ROI21_004172"
                     /pseudo
     CDS             complement(3574..4539)
                     /locus_tag="ROI21_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(4596..5219)
                     /locus_tag="ROI21_004173"
     CDS             complement(4596..5219)
                     /locus_tag="ROI21_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113028.1"
                     /translation="MKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFL
                     LQNDTFYQLRIKPNALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPV
                     TMLDGDDRVEKPEGVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNAP
                     LLVFGNYHNGKIACFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(5216..5353)
                     /locus_tag="ROI21_004174"
     CDS             complement(5216..5353)
                     /locus_tag="ROI21_004174"
                     /inference="COORDINATES: protein motif:HMM:NF018757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113029.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGAPGYSS
                     V"
     gene            complement(5367..6580)
                     /locus_tag="ROI21_004175"
                     /pseudo
     CDS             complement(5367..6580)
                     /locus_tag="ROI21_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
     gene            complement(6776..7114)
                     /locus_tag="ROI21_004176"
                     /pseudo
     CDS             complement(6776..7114)
                     /locus_tag="ROI21_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8631..9176
                     /gene="cobU"
                     /locus_tag="ROI21_004177"
     CDS             8631..9176
                     /gene="cobU"
                     /locus_tag="ROI21_004177"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDX5113030.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            9173..9916
                     /gene="cobS"
                     /locus_tag="ROI21_004178"
     CDS             9173..9916
                     /gene="cobS"
                     /locus_tag="ROI21_004178"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDX5113031.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGESILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            9928..11007
                     /gene="cobT"
                     /locus_tag="ROI21_004179"
     CDS             9928..11007
                     /gene="cobT"
                     /locus_tag="ROI21_004179"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDX5113032.1"
                     /translation="MQILADLLNTIPAIDSTAMSRAQRHIDGLLKPVGSLGKLEVLAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AEQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     QELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            11069..12004
                     /gene="ldtA"
                     /locus_tag="ROI21_004180"
     CDS             11069..12004
                     /gene="ldtA"
                     /locus_tag="ROI21_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDX5113033.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPDTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
BASE COUNT         3131 a         2647 c         3027 g         3211 t
ORIGIN      
        1 aaaatcctgc tgcgatgcac ctggcttgac ccggcagaga aagaggcccg tcgtttgcat
       61 ctgcagggta tgacggttcc ggattatacg gtgaaggtga gaaagtggtt ttgtgtgatg
      121 tgtgacgaaa attatgaggg ggaaaagtgc tgcccgagtt gtggtaccgg ggtatacagc
      181 agggaggggg ggcaggaggg caactggaag gataggaact gagcggttca gtcgcggaaa
      241 tcagttgcag agctgtaatg atatctccgg gagtgctgcg ttggtagggg catcccggga
      301 tagagaaatt ttagcacttt gccctgaatg attgtagacg tcccgagtac tcgtaccggt
      361 ctgagttatt acagttagat attcactttt tctcccgcat acttcacatc tgtcaacgac
      421 ggatgaaaag tggttcttcc gcaacctggg tggaagaaaa tggatgaaac tttctggcga
      481 acaaggatct aagctgtttt aagttatggg caacgcaatg cactctctta agacacttct
      541 acagttacct tgcggatggc gatgcagtcg acaaattatt agctctgacg gtataaccct
      601 ccatctccac ggaaaacgca aaacagcaca atgtcctgaa tgctttaagc gtagcgactc
      661 tgttcatagt tgtcgtcggc gccggataca gcatctaccc tgctcagggc agacgctatg
      721 gcttgtattt tccgtccgcc acgggtactg ccgtaacccg gcttgttcac gtaaaatttt
      781 tgccgagtcg cttgctccct tcgccagttc acaccagcag tcttcacagg cgttacaaaa
      841 ttaacaacgt caactgggat taatagctgt aggtgaggct ggaaaacggg ctgcaacggc
      901 agtgggtctc cgttgcagtg ctgatactct tcttcgcagg cttatcaata ccccggagac
      961 gaaacagtca ggcgcgcctc atgtcggtat tgatgagtgg gcgtggcatc ggggccactg
     1021 ctgcggtatg ttaatagtca atcctgatac tcaccgtccc ctcgtcctgc ttcccggccg
     1081 tgatcagcgt acgctggcga cctggttcag aaaatatccg gaaatacagg ttgtctcgcg
     1141 tgatcgcagt ggagtctatg cgacagcagc acgtgaaggt gcacctcagg ccagacaggt
     1201 ggccgatcga tggcacctgc taaaaaatat tggtgatgag cctgaacgaa tgatgtacag
     1261 atatatgcct ctgatacgtc ttgttgtcag agagttatca ctgaagaaat cacctgagcc
     1321 agaaatatct gtgcctgtag catcgctccg tcgtctggaa cgccttaaac agcacatccg
     1381 caaaaaacgg catcagcgtt ggactgaggt tatggccctg cataacaagg gatgtagttt
     1441 cagggaaata tcccgtatta caggcctgtc gcgagtgaca gtcagtcgct gggtgggttc
     1501 aggaacattc cctgaaatgt caactaggcc tccaaagcga gggcttctgg acccatggag
     1561 ggagtggtta aaagagcaac gagaatgtgg taattataac tccggccgga tatggcggga
     1621 aatggtggcc aggggggtta caggcagtga aaccatcgtc agggatgctg ttgccaaatg
     1681 gcataaaggc tggatcccac cggttactac tgccgcaaga cttccttcag tgtcccgggt
     1741 aagccgctgg ttgatgccct ggagaataat caggggtgaa gaaaattatg ctttccgatt
     1801 tattagtctg atgtgtgaaa aagaaccgga gttgaaaata gcgcagcaac tggtactcga
     1861 gttctaccgt attctgaaaa cctaaaataa atcacagcta agcagctggt tcactcgggt
     1921 ccacgaaagc ggctcagcag aacttcggtg cgtagctgcg gggatggaag ctgatgctgc
     1981 ggctatatgt gaggcaatca gcagtcgctg gagtactggt gttgtcgaag gtcatgtaaa
     2041 tcgcctgaag gtgctgatac gccagatgta tggtcgagcc ggacttgaac tacttaggcg
     2101 gagggtaatg agtccactgg catgaaaatc ttcacccagg ttgaggaaga gccacttttg
     2161 cactgttcgt gacagtatgg cctttatgta ctcactttgt taccggtagc tggatcatca
     2221 ctaccgtgcc gcaaatggtg gtgaacagca tcaattgtgg aatgaatgtt gccgaagcga
     2281 ccaatgcgcc tcgtttccac catcagtggt tgccggacga gctgcgtgtt gaaaagggtt
     2341 tagtccggat acgctcaagc tgctggaagc aaaaggtcag aaagtggcgc tgaatgaggc
     2401 gatgggcagc acgcaaagca ttatggttgg gtcggatggt gagttgtacg gcgcatccga
     2461 ctcgcgcttg gtggatgatt taacggcggg gtattaattg acatcccccg gcgcaatgtg
     2521 ccgggggata atggttttat gcggtcaata atcgtccggg attatcgata cagagttttt
     2581 tgaggatcgt tttatcgacg ccgcgctccg ccagataggc cagaaaaaca tctggaacaa
     2641 atccccagcc attaccgcca tttttagccc acatttgttt aaggaagaca tcgtggctga
     2701 gaacaagctg atcggcgtat cccaactcaa tgagatgagc gacggcatcg gcagtctcct
     2761 gcacccctgg cgctattccc tctttcggga aggtaatgtc taggccaatc atgtcgaatt
     2821 ccagccagac acctttatca agcattttcc gctgatacgc cacgtctttt cctgacggat
     2881 ccgagtgtgc gagagagact ttatttggcg agacgcccat ttcacctaac acaatgtcga
     2941 gtacttcatc accgcgacga agccagcccg gcatgtgaat attcatcgcc acatgaggat
     3001 tgttaatctg tgccagcgag gcagcccgca agctgttatg ctcggcttcg gtaaatgtcg
     3061 gtgagacacc tatttcaccg atcattccgg cacgaatatc cgtatcgcca atcccttgat
     3121 tcaattcttt atcgatcgtt gtcgctagtt catcaaccgt tttatgaatt ctctggcttt
     3181 caaatttttc caggtaggga ccggaggaag caacaatatt cagacctgtt tttaatgcga
     3241 cttctcgtaa tgcctgcgca tctcgtccga tagactcaga ccctgtggca tcaacaatgg
     3301 tgcgaccacc gagcgagata aagttattaa tttcgaagat cacatcttca ataggtttgc
     3361 ggtccatatt atcagcgcag caataaggat cgtgttttaa tgcccactgg atttctgccg
     3421 tgactttttt atcaacaagc tgttgtgaaa aaggatagca aggagcatcc acaacagagg
     3481 aaagatcgtt gaacagatgt tcatgaggaa gtgttagccc catatcttca cgggcgacgg
     3541 ggcccgtcac agtttgtaga taatctttca tgattatacc tccattttct tcattgtttt
     3601 cgaggctctg tattccggaa tagtggtaac aattgcaccg accagtgcaa acagtgttcc
     3661 gatgatcgtg accagataaa cggtattgcc cagcgatgga attaatacgt caatcaatac
     3721 tgatcccagt aactggcccg ccgttgaagc aacccccagc atcagtaacc ccagacctct
     3781 gaccaaaatg gccattaaac cgatagacat tagtccaagc gggccgccaa gatacatcca
     3841 ccagacatct ggtaattgaa cagtgacgtg ccctaatgcg acacgaatag ctagcgctgt
     3901 gcctaaaaca caaaatccaa cgatgaaatt ctaggtgatt gaaaccagca tggatcccgt
     3961 agcttcagct acttttgcat ttccagcggg ctgccatcct gcaagcaaac cagctaagaa
     4021 cggaagaatg gcaagtaaga tgaaagaggt agaatgccac tgaggtgaca caacaaaaat
     4081 cgtagcaata accgcaaata atgcaccgat gatgcgataa ggggtaaagt attttttctc
     4141 gtcgacaccg ataccaaagc gatcgcaaag taaaccagac agcaatagcg cggagatcag
     4201 tgcggtttga aacgttgcga ccccgagtgc gctggctgat gcgccttcag aaaataccac
     4261 catcgctcca cagagaccgg caaaccagtt ccagagagga acttttcggt ttttaatcag
     4321 agaaggaatt gccgcaaatt gttgccgggt ttcttttcgc gccataataa taaaaaacat
     4381 cacgactaaa ccactggcaa aggagataac tgcgcatgca ttaccatcct gtaaccagtg
     4441 acctaattga ccattcaccg ctgattgcat aggggagagc atacccgcaa gaatcgtggc
     4501 aagcatccat aagggagttg agtacttatt ctttttcata catcaatcct tctattatag
     4561 taaatggagt aactggagtt accccattat tattattatc ttgcaatatg agtcagtata
     4621 tttacccaca acgcggtata gaacggccag gacataaact gctgtgtacc ccagtgcggt
     4681 gaacagtcac tcataaagca ggctattttc ccgttgtgat agttaccaaa gaccagtaat
     4741 ggagcgttgt ttattgttaa taccacctca gcattttctt tagcgatcgc ccggttataa
     4801 cctaggaaaa aaggatattc agagaaccct ttaataactg gatgttcagg ttgtgatggc
     4861 tgtgcaataa caccctctgg tttttcgaca cgatcgtcac catctaacat cgtaaccggg
     4921 agcacatcag ccagaactgt atttttataa ttggcttttg cttcaattcc catgaatgag
     4981 agatagccgc caatcatcaa taacccacca ccgttattca catattcttt aatcagttcg
     5041 agagcgtttg gttttatgcg aagctgataa aaagtatcat tttgtagcag gaaagtatta
     5101 ctaccaatat cactaataac gatggcatca tattgttcta actgtgccac atcttcaggg
     5161 aaggcaattt gtaccgtatg tgctggcata taggtgacat ctacctgact atttttcaga
     5221 cactggagta accaggtgca ccttcttcat atttacttga ggtaaaactg tcatatcctt
     5281 ttgaatgaat catatgaata tgccaggatt ccccaataaa gagcactttt agtgattttt
     5341 tggtgttatt cattctctgt cctttcttaa tgcgtaaaaa tagtttgctg ataatgtata
     5401 ctctgaattc gatgtatcac agattcatgt tcaggcatgt cggaagcatt gcttgtctcc
     5461 accgctaggg atgcaaaggc gctggcataa cacagtgctg attccagtga ttttcctttc
     5521 gcaagtcctg atgccagaga gccgttaaac gcatccccgg ctcctgccgt atttttaacg
     5581 accgctggaa acgctggcga atgaatgaat tttttgccat caaatgcgag cgagcccttg
     5641 ctaccgagcg tgataaccgt gttttttacc cctttattgt ggatagcttc tgcagctcgt
     5701 ttagctgatt caagatcgtg tacgtctatg ccggacagta aaccagcttc tgtttcattg
     5761 ggggtgatat aatcaacatc ctgaataagt tcgttgacga tatcattata gggtgccggg
     5821 ttaataatga ccgggatact gtttttttgc gccaatgtga tcgcttgttg cagagcggta
     5881 taatttgttt cgagctgcag aagtatgaca tcagaattaa taatcgcttc tttctgaata
     5941 gttatttcat cggacgacat tgtcatgttg gcaccgggat agatcgcaat aacattatcg
     6001 ccagcacctt cattaacaag gatcgtggca gttccggttt gcgtttcttt cgtttgataa
     6061 attatacttt tataaatctt agacgaattc ataaagttga tcgcataatc actaaagtga
     6121 tctgtaccta cttttgttat gaaataaaca tcagtatcgg caaagcttgc tgccagcgcc
     6181 tggttacagc cttttccccc ggtgaaaata taaaattact tgccagaagc gattctccga
     6241 tagtgggaag cctcggcaaa taactgatga tatcaacatt aaacgaacca ataacgcata
     6301 cttgactttt catagttgag tggccttttt ttattccctg ggctgtatca taattttcaa
     6361 gcgcataagc gatttcattt ttcgcaatag tatcaagcga gtctaggacg ataaaagcac
     6421 cgccatgaca tcgagtcaga tagccaagcc tggcaagttc attaagatct gaacgaatag
     6481 tttctttcgt tacattgagg ctattggaca gatcggatac ttttacagaa ccgaatttac
     6541 tgagtcgttt caatatttca tgatggcgtt caaatttcat gcttcacctc acttcagtta
     6601 cgttaatatc tgtcgatgta tattgatgtg ataatcacca taaagtggcg gggaaaagca
     6661 aaaaaaagca aaaatagcct gagaaaaggt aagcgttaat ggagaatcat attgatgctt
     6721 atttattggt gagaactacg ttccatggca ggagttcatc aatacggttg gaaggttatt
     6781 ccggcagtac gctcaggata tggcgcagat acgcttccgg atcaatgtcg ttcagaaggc
     6841 aagtgccgat aagcccatac aacagtgcac tgcgctcgcc gccgaagaac ataaagtttt
     6901 tatttccgag acagatggca caaagggctt tttccgcggc attatgtctg cctccgcctg
     6961 accgtcaaca ctgtaataac agagtgcgcc ccactgattc agtacacagc tgaaccattc
     7021 tcccagtctg gatttttgga tagcgtggca tttttctcca ccatgcatgc agtgagtcca
     7081 gtaacagttt gctcttcttc tgcctgacca caagatgtgc tggactggta aacgatgggg
     7141 atacctgctt acgcgagagc aataagtggc tggccacaat tctcggtaca aaactctgct
     7201 gaatagcagc gattttccca gcgtggtgcg tcgtttgcat tttactggtt ctaagttaac
     7261 aattatctgt tcactaatga ctactctaaa aacttgtatc aggactatca cttatttaag
     7321 tgatattggt tgtctggaga ttcagggggc cagtctataa actttttaca aaaggatgaa
     7381 tgcagaaggt tactctaatg gccagagtga tgacaatgat ggtcagacgt tgacctgcag
     7441 aaactgcatt ttaattggtc tataaagtct gcaagaggaa tcagggtcag atcagtgagt
     7501 cttatcgcgg cccgaagcta tgactgaaag aaggctggat acagaattgc aacaatttgg
     7561 caatgtcaga acacagaagc tttgcaagca ggtgaaaggt tatgtctagc aaaaggagta
     7621 tatcagaata catctactga tgtacaaaac acaacaatag cttcatgata tataaatacg
     7681 tgatgacgag tggtactccg catggttatt taacttctca gcaccactaa tgatcctgag
     7741 caacttcgta tttgggccat tgccatggag tagaaagctg tgtctgagaa tgcagaattt
     7801 gagaaaagaa ttcgctttct tggagagctt ctgaaaatgg cacgctaaca acgttttgtt
     7861 aaaaaatgtg aatcacttgc cggggctgca ttttctattc gaggagaatg ttgacgtaga
     7921 tattacagaa atctcagcgc atcaggataa actgctacct taggtcaacc accaaaagaa
     7981 aatacaatac gtccagtttg tatgccttta ataacacatc ttttaaatgt tgaagaatta
     8041 tcccgcctgc tgaagaacgt tgctctgagt gtgataaacc tttttattct tgatgatgtc
     8101 agcgagaaga tggaatatat ccccgactgg gtcgtggtta cccggttaaa catctggtgg
     8161 cgctaataaa tctggcaagt cacattctgt tgaatgctgt gacagtgcct taccgagaga
     8221 gcgaaattgt ggtgttctag tgcatagtcg ccaccattcc ggataattct attgcgttgt
     8281 tttacaagtt cttttacagc gcagatctgt cgctgacgtt gaatcaatag cctggatttc
     8341 agaacacaat gcccgtttgc tggttggtcc ggcagtagca ttaattcatt atctgaatga
     8401 aatgtgtcaa tccacagaaa ccgctgtcaa tgacacattt tgtcggacgt taacacgaga
     8461 aagtctggct attccgtaat gccatcttat ttcgtaatgg attgagaatg ctggattttt
     8521 aattatatta cgcgcagtat taatcgcgta aatgttttgc ggtcagcaac ggtgggggat
     8581 ccattgttct gttcaatgaa taccatcata catttgaatc aggagtcggt atgatgattt
     8641 tggtgacggg gggcgcacgg agcgggaaga gtcgccacgc agaggcgctt attggggact
     8701 cttcacaggt tctgtatatc gccacctcgc aaatccttga tgatgagatg gctgcacgga
     8761 tagaacatca tcggcaaggc cgcccggagc actggcgcac agtggagcgc tggcaacatc
     8821 ttgatgaatt aattcatgca gacattaacc cgaatgaggt tgtgttgctg gaatgcgtta
     8881 ccacaatggt gactaatctg ttgtttgatt atggcggcga taaagaccct gatgaatggg
     8941 attatcaggc gatggaacag gcgattaatg ctgagattca gtcgttgatt gctgcctgcc
     9001 aacgttgccc cgcaaaggtt gtattagtga ctaacgaagt gggaatgggg attgtgccgg
     9061 agagtcgtct tgcacgacat tttcgtgata ttgccgggcg ggtaaatcag cagttggccg
     9121 ctgcggcaaa tgaagtatgg ctggtggttt cgggtattgg agtaaaaatc aaatgagtaa
     9181 attattttgg gcgatgctct catttattac gcgtctgcct gtaccgcgtc gctggtccca
     9241 gggactggat ttcgagcatt attctcgcgg tattattact tttcctttga ttggattatt
     9301 gcttggcgcg attagcgggc tggtcttcat ggtgctgcag gcatggtgtg gcgcaccact
     9361 ggcggcactg tttagcgtac ttgtgctggt gctgatgacc gggggattcc atctggacgg
     9421 cctcgctgat acctgtgatg gcgtattttc tgcacgtagc cgcgatcgca tgttggaaat
     9481 catgcgtgat agccgtttag gcacccacgg cggtctggca ttgatttttg tggtactggc
     9541 aaagattctg gtgttaagcg agttggctct gcgtggcgaa tcgatccttg catcgctggc
     9601 ggcagcatgt gcggtcagcc gcgggactgc cgcattattg atgtatcgtc atcgttatgc
     9661 gcgggaagaa gggcttggca atgtgttcat tggcaaaatt gatgggcgac aaacctgcgt
     9721 cacactcggc ttagccgcta tttttgctgc cgtattattg ccgggtatgc atggtgtggc
     9781 tgctatggtg gtgacgatgg tggcgatttt catcctcggt cagcttctca aacgtacgct
     9841 gggcgggcaa acgggcgata cgctgggagc agccatagag cttggtgaac tggtcttcct
     9901 gctggcactg ctataaagag acgtgagatg caaatacttg ccgatttact gaatacgatc
     9961 ccagccatcg attctaccgc tatgtcgcgt gcacaacggc atattgacgg gttactcaaa
    10021 cctgttggta gcctgggaaa gctggaggtg cttgccatac aactggcagg aatgccgggg
    10081 ttgaatggca taccgcatgt gggcaaaaaa gcggtactgg ttatgtgtgc cgatcacggc
    10141 gtctgggagg aaggggtcgc tatttctcca aaagaagtga cagccataca ggctgaaaat
    10201 atgacccgtg gaacaaccgg cgtgtgtgtg ctggcagaac aagcgggcgc taacgtccac
    10261 gtaattgatg ttggtattga tactgctgag cctatccccg ggcttatcaa catgcgtgtc
    10321 gcacgaggta gcggcaatat tgcttcagct ccggcaatga gtcgccgtca ggctgaaaag
    10381 ttgcttttgg acgtcatatg ttatacgcag gagctggcaa aaaacggtgt cacgctgttt
    10441 ggtgtaggtg aactggggat ggcaaacacg acaccggcag cagcaatagt cagcacaatc
    10501 actggccggg atcctgaaga agtggttggg attggcgcaa acctgccgac agataaactg
    10561 gctaataaaa ttgatgttgt gcgtcgggcg attacgttga atcaaccaaa tccgcaggat
    10621 ggtgttgatg tcctggcaaa agtgggtgga tttgatttgg tcggaatagc tggagtgatg
    10681 ttaggtgctg cttcctgcgg tttacccgtg ttgctggatg gatttctttc ttatgctgct
    10741 gcgctcgcag cctgccagat gtctcctgcg atcaaaccgt atctcattcc ttctcacctg
    10801 tcggcagaaa aaggcgcgcg tatagcgctc tcgcatttgg ggctggagcc ttatctcaat
    10861 atggagatgc gtttaggtga ggggagtggc gcagctctgg cgatgcccat catcgaagct
    10921 gcctgtgcga tatacaacaa catgggcgaa cttgctgcca gtaatattgt tctaccgggg
    10981 aatacgactt ctgatttgaa cagttaaaaa cgaaaagtct gataaaagct atacttaacg
    11041 gatagctttc gcgacatagg aaagggacat gatgcgtcgt gtaaatattc tttgctcatt
    11101 tgctctgctt tttgccagcc atactagcct ggcggtaact tatccattac ctccagaggg
    11161 tagccgttta gtggggcagt cgtttactgt aactgttcct gatcacaata cccagccgct
    11221 ggagactttt gccgcacaat acgggcaagg gttaagtaac atgctggaag cgaacccggg
    11281 cgctgatgtt tttttgccga agtctggctc gcaactcacc attccgcagc aactgatttt
    11341 gcccgacact gttcgtaaag ggattgttgt taacgtcgct gagatgcgtc tttattacta
    11401 cccaccagac agtaatactg tggaagtctt tcctattggt atcggccagg ctgggcgaga
    11461 aaccccgcgt aactgggtga ctaccgttga acgtaaacaa gaagctccaa cctggacgcc
    11521 aacgccgaac actcggcgcg aatatgcgaa acgaggggag agtttgcccg catttgttcc
    11581 tgcgggcccc gataatccca tggggctgta cgcgatttat attggcaggt tgtatgccat
    11641 ccatggtacc aatgccaatt ttggtattgg gctccgggta agtcagggct gtattcgtct
    11701 gcgcaatgac gatatcaaat atctgtttga taatgttcct gttgggacgc gtgtgcaaat
    11761 tatcgaccag ccagtaaaat acaccactga accagatggc tcgaactggc tggaagttca
    11821 tgagccgttg tcgcgcaatc gtgcagaata tgagtctgac cgaaaagtgc cattgccggt
    11881 aaccccatct ttgcgggcgt ttatcaacgg gcaagaagtt gatgtaaatc gcgcaaatgc
    11941 tgcgttgcaa cgtcgatcgg gaatgcctgt gcaaattagt tctggttcaa gacagatgtt
    12001 ttaagagcgt tggtaa
//
LOCUS       DAPOZO010000131        11908 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPOZO010000131 DAPOZO010000000
VERSION     DAPOZO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.131"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..836)
                     /gene="pdxK"
                     /locus_tag="ROI21_004181"
                     /gene_synonym="thiJ"
     CDS             complement(<1..836)
                     /gene="pdxK"
                     /locus_tag="ROI21_004181"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDX5113034.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPP"
     gene            941..1309
                     /locus_tag="ROI21_004182"
     CDS             941..1309
                     /locus_tag="ROI21_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDX5113035.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKFGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(1312..2223)
                     /gene="cysM"
                     /locus_tag="ROI21_004183"
     CDS             complement(1312..2223)
                     /gene="cysM"
                     /locus_tag="ROI21_004183"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDX5113036.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEIWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQREAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2357..3454)
                     /gene="cysA"
                     /locus_tag="ROI21_004184"
     CDS             complement(2357..3454)
                     /gene="cysA"
                     /locus_tag="ROI21_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDX5113037.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3444..4319)
                     /gene="cysW"
                     /locus_tag="ROI21_004185"
     CDS             complement(3444..4319)
                     /gene="cysW"
                     /locus_tag="ROI21_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDX5113038.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4319..5152)
                     /gene="cysT"
                     /locus_tag="ROI21_004186"
     CDS             complement(4319..5152)
                     /gene="cysT"
                     /locus_tag="ROI21_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDX5113039.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5152..6168)
                     /gene="cysP"
                     /locus_tag="ROI21_004187"
     CDS             complement(5152..6168)
                     /gene="cysP"
                     /locus_tag="ROI21_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDX5113040.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6339..7130)
                     /gene="ucpA"
                     /locus_tag="ROI21_004188"
     CDS             complement(6339..7130)
                     /gene="ucpA"
                     /locus_tag="ROI21_004188"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDX5113041.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7259..8116)
                     /gene="murR"
                     /locus_tag="ROI21_004189"
     CDS             complement(7259..8116)
                     /gene="murR"
                     /locus_tag="ROI21_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDX5113042.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKLL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            8280..9176
                     /gene="murQ"
                     /locus_tag="ROI21_004190"
     CDS             8280..9176
                     /gene="murQ"
                     /locus_tag="ROI21_004190"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDX5113043.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALVACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            9180..10604
                     /gene="murP"
                     /locus_tag="ROI21_004191"
     CDS             9180..10604
                     /gene="murP"
                     /locus_tag="ROI21_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDX5113044.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            10609..>11908
                     /gene="pbp4b"
                     /locus_tag="ROI21_004192"
     CDS             10609..>11908
                     /gene="pbp4b"
                     /locus_tag="ROI21_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDX5113045.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPDIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
BASE COUNT         2752 a         3340 c         3076 g         2740 t
ORIGIN      
        1 ggcggcaaaa tcaattcatc gctctcatgc tgctgggtgt agcgcatcac ttccagtacg
       61 cgcaaccccg cacggtgcac cgcatcgttt aacgccttcc ctttcagcaa accactgata
      121 agctgagcac aaaacaggtc gccagtccct ttcaggtcgg tttttacccg tgaatgggaa
      181 atgacattca cgctgtcggc actgaccacc accacctgca tctcctgatt ttcttcatta
      241 ccggaggcgc tggtaatcac cacccatttt aatgtgtctg aaagcagact ttttgcggca
      301 gcaatggcac tgtcgagatc gcggcaattt ttaccggtca ggatttccag ctcaaagata
      361 ttgggggtaa ttccctgcgc cagcggcagt aaatattgtc gatacgcctc gggaaggtca
      421 ggtttgacat aaattccgct atcaatatcg ccaatcactg gatcgaccat gatcaatagg
      481 tcaggatgat ctttgcgtag cgcagtcagc cactcggcaa ggattttgat ttgcgatgcc
      541 gttcccatat agcccgtggt tacagcacga agttggcgca acgcatcacg ctcctgaagc
      601 gcacgcaaat agccgctaaa ccattcgtcc ggaatcgcac caccgtagaa agtgtcataa
      661 tgcggtgtat tgctcagcaa taccgtcggc acggcaaaga cattcaggcc gttctgtttg
      721 atagcaggca cggcaatgct gttgcctacg ctgccgtaca ctacctgcga ctgcacggcg
      781 acgatatctg cctgcaatgc cctgctctta tcgttaaaca acaacaaact actcatttaa
      841 ttttttctcc ttgccgatga tcctcatcgt attccaaccg aaactttacc tgattctggc
      901 agtcaaatcg gctatcacaa aacaaggata aggtaattca atgaagaaaa tcatttgtct
      961 ggtcattacg ctgctaatga cactccccgt ttacgcgaag ttaactgccc atgaagaagc
     1021 ccgtatcaac gccatgctgg agggattagc acagaaaaag gatttgatat ttgtgcgtaa
     1081 cggcgatgaa cacacctgtg atgaagcggt ttcccacctg cgtctgaagt tcggcaacac
     1141 gcgcaaccgc attgataccg ccgagcagtt tattgataag gtggcctcgt cgtcatcgat
     1201 taccgggaag ccgtatattg tgaagatgcc cggtaagagc gatgaaaatg cgcaaccttt
     1261 tttacatgcg ctgattgcgc agacggataa aacggtgcct gcgcaataat cttaaatccc
     1321 cgccccctgg ctaaaatgct cttccccaaa caccccggta gaaaggtagc gatcgccgcg
     1381 atcgcagatg atcgcgacca ccaccgcgcc ggggttagct tttgccacgc gcagtgctcc
     1441 ggcaaccgcg ccgccggagc tgacgccaca gaatattcct tcccgcactg ccagttcgcg
     1501 catggtgttt tccgcttctc gctgatgaat atccagcacc tcatccacca gagaagcgtt
     1561 gaaaatcccc ggcagatatt ccgcaggcca gcggcgaatg ccgggaatgc tactgccctc
     1621 ttccggctgc aggccaacaa tggtcaccgg tttggattgt tcgcgcataa agcgtgaaac
     1681 gccggtgata gtgccggttg ttcccatgct ggagacaaaa tgggtaatgc gcccgccagt
     1741 ttgctgccag atttccggcc cggtggtggt gtaatgggcg tagggattat caggattatt
     1801 aaactgatcg agcagctttc cttcaccacg attcgccatt tcaagcgcca gatcgcgcgc
     1861 accttccatg ccctgctctt tggtgacaag aatcagttcc gcaccataag cacgcatcgc
     1921 cgcacggcgt tcctggctca tgttgtcggg catcagcaat ttcatgcgat agcctttaag
     1981 cgcggcaatc attgccagcg caatgccggt gttaccactg gtggcttcga tcagcacgtc
     2041 ccccggttta atttccccgc gcttttccgc ctcgacgatc atcgaaagtg ccgcacgatc
     2101 ttttaccgaa cctgccgggt tattgccttc cagtttcaac cagatttcac tgccgttatt
     2161 aggccccatt cgctgcaact tcaccagagg cgtattgcca attgtttgtt ctaatgtact
     2221 cacgatctct gtccatacgt ggtgttgcct gatgcgacgc ttgcgcgtct tatcaggcct
     2281 acaggttaca aaccttgcca ttaaaaagcc cggatcgcgg gaagcgcctc cgggcgttta
     2341 acattcactc aacctatcag gcgctttgtg cgagagcaag ttcctcatcg cgggtttcaa
     2401 tacgctcgtc gccgttatac agccgcgcat gttgcagacc aacgaataaa cgctcgccac
     2461 gttgcggggc gtcgtcgccg tgcatcacga ccgtcagcgg ttcgttgtac caccccagcg
     2521 gctgcacgac taattgggtg tagtggcctt tcgggctggc ttccagtacc tgtaccggca
     2581 gcggcgaatc gaggctggta cggcggctga tatccacttc ccacggacgc aggaagagat
     2641 ccactggccc ctgatacgca ggtgtatagc ccagcggcca gcgatgtgcg ccaacatgga
     2701 actgcccgcc gcgaatggtt ccctgcaggc ggttcacttc gcccataaat tcgagtacaa
     2761 aacgggtcgc tggttcgcgc catacctggt ccggtgcatc agcctgctcg atattgccct
     2821 ggctcatcac cactacacga tcggctactt cggtcgcctc ttcctggtca tgggtcacga
     2881 acacgctggt gaatttcagc tcttcatgca actgacgcag ccagcgacgc agctctttac
     2941 gcacctgagc atccaacgcg ccaaacggtt catcaagcag cagaatttgc ggttctacag
     3001 ccagcgcacg cgccagtgcc acacgctgtt tctggccgcc ggaaagctgc gccggataac
     3061 gatccgccag atgggcaagc tggaccatct cgagcaattt tgtcactttc gctttgatag
     3121 ctgcggcatt cgggcgctcg cgacgcggca gcaccgtcag gccgaaagcg atattgtcga
     3181 acaccgtcat atggcggaac agcgcatagt gctggaacac gaaaccaact ttacgatcgc
     3241 gcgcatgcag gcggctgacg tcggtgccgt ggaagcgaat atgcccgctg gtttgatgct
     3301 ccagcccggc gataatgcgc agcagcgtgg ttttcccgga accggacggc cccagcaacg
     3361 cgaccatctg accagaagga atatccagtg agatatcgtt cagcacctgg gtgcgaccaa
     3421 aagacttctt aatattggca atctcaatgc tcatgatgtt cctcctgctg cgcgcgtttt
     3481 tcctgattct ccagacgcca ctgcaacata ctctttaaaa acagggtgat aatcgccatc
     3541 agcgtcaaca acgcggcagc ggtaaaggag ccgacggtgt tgtagtcttg ctccagcaat
     3601 tcaatctgta acggcagcga aagtgtttcg ccgcgaatcg agccggaaac caccgacacc
     3661 gcaccaaact cgccaatcgc gcgggcgttg gtcagcacca cgccataaag cagcgcccag
     3721 cggatgttcg gcagcgtgac gcggcgaaac atctgccagc cagatgcgcc cagcaaaatc
     3781 gccgcttcgt cttcctggct gccctggctt aacatcaccg gcaccagctc acgcaccaca
     3841 aacggacacg tcacgaagat ggtgaccagc accattcccg gccaggagaa cataatttgc
     3901 agattatgtt catccagcca gccgccgagc gggccgttag agccgtaaaa cagcagatac
     3961 accagaccgg caaccaccgg cgacacggca aacggaatgt ccagcagcgt cagcagcaac
     4021 tggcgtccgg ggaagttaaa gcgcgtcacc agccatgcca gcagaatgcc gaacaccagg
     4081 ttcaccggca cggcaatcag cgcaatcatc accgtcagcc agatggcgtg cagcatgtcc
     4141 ggatcggcca gattctgtaa aactggcatc agccctttac tgaacgcctg cacaaagatg
     4201 taaatcatcg gcaccagcag gatgaacgcc gaaaccagca tcccgatgcc aatcagaaac
     4261 catttgcccc agttaatcgg gcgcgcgtca taacgcttca actgtgtcac ttccgccatt
     4321 aatgacctac cacacgccga ccaaagcgac tttgcagagt gttaattgag aacagcagca
     4381 gcagggatgc cgcgaggatc accgaagcaa tcgcgctcgc cgccgggtaa tcaaactcct
     4441 gtaagcgaac aaaaatcatc agcgacgtca cttctgtctt ccaggcgatg ttcccggcga
     4501 taaaaatcac cgcgccaaat tcaccaagac tacgggtaaa cgacagcgcc acgcccgcca
     4561 ccagcgccgg agatagctcc ggcagcacca ctttgcagaa actctgccag cgcgttgcgc
     4621 cgagcgtttc cgccgcttct tcatattccg ggcctaactc ttccagcacc ggctgcacgg
     4681 tacgcaccac aaacggaatg ctggtaaagg ccatcgccac cgcaatcccc agccaggtat
     4741 aggtgacttt gatatcaaac ttcgccagcc attcaccgta aaaaccgttc acggaaaaga
     4801 gcgaggccag cgtcaaaccg gctaccgccg tcggtagcgc aaagggtaaa tccatcagcg
     4861 catcaagcag cgtgcggccc ggaaagcgat aacgggttaa aatccacgcc atcagcaggc
     4921 cgaaaacgcc gttaaaaatt gatgccacaa acgccgacag cagtgtcact ttgtaggccg
     4981 cgactacctg cgggttggtg atcacctccc agtactgcgc ccagctcatc tcagagagct
     5041 gcatcaccag cgcggagagc ggcagcagca aaatcaggca cacaaacagc agactggtgc
     5101 cgaggcttaa ggtaaagccc ggcagcacgc gtcttgatga aactgcaaac attaattacg
     5161 ccccgccgct aacagcttgt ccaactcgcc gccgctggtg aagtgggttt tcatcacttc
     5221 cggccaggag ccaaatttgt cttccacgcg gaacagttcg gtctgcggga atttgtcttt
     5281 cagtttgtcc atgacttccg ggttatttac gcggtagtaa tagtcggtga tgatggtttg
     5341 cgcctgcggg ctgtagagcc agttcaggta agcttttgct gctttttccg taccgttggc
     5401 ctgcacgttt ttatcgaccc acgccaccgg gaattccgcc agaatattgg ttttcggaat
     5461 caccacttca aagccctgcg cttcatactg tttacgaatg ttgtttactt ccgattcaaa
     5521 gctaatcagt acatcgccca gcccgcgctc ggcaaaggtg gtggtcgcgc cacgaccgcc
     5581 ggtatcgaac acttcaacgt ttttcaggaa ctgggtcata aactgttcgg ttttggcttt
     5641 gtcgccaccg tcagctttat ccgctgcacc ccatgccgcc agataggtgt aacgtgcgtt
     5701 acccgacgtt ttcgggttcg ggaaaatcag ctttacgtcg gagcgcacca gatcgttcca
     5761 gtcgtggata ttcttcgggt tacccttacg caccaggaag cccatggtgg agtagaacgg
     5821 cgagctgtta ttcggcaggc gcgtttgcca gtcggccggg atcagcttgc ctttgtcgtg
     5881 caggatttgt acgtcagtca cctggttata agtgacaacg tcggctttta agccctgcaa
     5941 aatcgccagc gcctgttttg atgacccggc atgagattgt tttatcgtca gtttgtcgcc
     6001 gccgttatct ttggcccatt gttgctcaaa cggcggattc agggcggcaa acagctcgcg
     6061 ggagacgtca taagaactgt tcagcagttc cgttgcctgt acatggcccg ccagcagcag
     6121 agaagcgacc agcgcgagtg agttcttttt cagtaagtta acggccattg cgcaccctta
     6181 taaatttaat gactttctaa tagccatcat atttataacg gacgtgaaag gagtaacggt
     6241 tttatatacc gtttggtgat ttggaagttg aaaagggaat aagaaccggg ttcaagacga
     6301 aatcctcccc acaaatgcag ggaggaaaca gaggtgaatc agatgccgac gctaaccgtc
     6361 tccggcagtg tgctgccgcc atcaatcaca ttctgtgtac cggttaaata acttgattca
     6421 tccgatgcga ggaaggctgc cagttcgccg acttccagcg gatcggcgag gcgacgcatc
     6481 gggattgctt ttgccatttc agtcagcacc gattctggat cttccgggtt cgactggcgg
     6541 gcaatgcttt ccgccattgg cgtgcgtacg tatcccgggc agatggcgtt aacgcgaata
     6601 ccagactgcg cgtactccac cgccagcgat ttagtcaggc caacaatcgc cgctttcgtt
     6661 aaggcatacg ccgtttcacc cggatcggcc accatatcgc cagtgactga agacatcatc
     6721 acaatgcgac catctttgcg ggcaatcatc tccggcagca ccgccttcgt gacgttccat
     6781 acgcctttaa tattgatatc aatatggaaa tcgcgatctt catcgctcat atcgaggaaa
     6841 ctgcccagac gacaaacgcc tgcgttattc accaggatat caatgcgccc ttctttttcc
     6901 ttcgcgcgct tgatagctgc ggcgaccgac gccgggtcac gcacatcggc gacaaccgcc
     6961 gtacagcgat gaccacgacc acacagttcg tccgccagct tttcgatctc aggggagata
     7021 tccagcaaga ttaggtttgc gccatgacgc gcaaaagttc tggcaattcc ttcgccaatt
     7081 ccctgcaatg cgcccgtaat cagtgctgtc ttgcccgtga gtttacccat tttaatgctc
     7141 tccttgttaa tacagagcgt tacactccat tccctctgaa aatacaggat aaacaaaata
     7201 ttgctgcgga tcagctcact aattcaggaa aatctcaatc cagtcgctgc tttatgcatc
     7261 atttcaggcg ttgcgttagc tcactgctgc gctcaatcat tttcaatgac tccacatcat
     7321 tgagttgcac aaggccaaca aacagtaaat cggtaacaga attttgcgcc gtgcgggtgg
     7381 acatcgacga gctgcgccat tcggtttcgc cagaaacggt atcgagagtg taatgcgcca
     7441 gccgccgcag cggggaatcg gttaatgagg taatggcaat caccgttgcc ccctgctttc
     7501 gcgctgcctc ggcgcacaag acaatctctt ttttactgcc gctgtaagag atagcgattt
     7561 gtacatcgcc ttttttcagc gcctgggaaa cggtggcttg tacatgcgtg tcagcttcac
     7621 aggccacacg ataaccaatt ttcatcagtt tgaatgaaag atcgcgcccc accagcgccg
     7681 acccgcccag gcccgttatc tggataaatg gcgctttact gatgacctca atgatttttt
     7741 gcagccgcgc gtagtcgaac aacgcgcagg tttgctccag cgccagctct ttttcgcgat
     7801 tcagtttgcg ggcaatcact tccagcgaat catcactggt aatcgaactg tgcaggtgca
     7861 gcgccgtggc attcgttttc tcccggctgg cactgtattc gccaattaac gccatacgca
     7921 attcagtaaa tccttgcgcg ccgagcttct gggcaaactt cacaatactc gactggctga
     7981 tgccgagcag tttcgccatc ttgcgcgaag agacagattt cagctcactc acattagccc
     8041 gcagaaagtc agcaattttt tgctcgttcc cggtaaattc actttccgca ttgcgtattt
     8101 tcgtcaggta caacatcttc cactctcctg ggctggcagc aaaaattccg cttaagttct
     8161 tatgacgctc ttcacactct gcgagtgtag actcaatgat tcctttaagt ctgtcaattc
     8221 gggatatatg attccataca tctgcaatta tttgatcgta aatagtaagg tcaccaccga
     8281 tgcaacttga aaagatgatt accgaaggct cgaacgccgc ctcagctgaa attgaccgcg
     8341 tatcaacgct ggaaatgtgc cggattatca acgatgaaga taaaaccgtg ccgcttgccg
     8401 ttgagcgcgt actgccggat atcgccgcgg cgatcgatgt tatccacgcc caggtaagcg
     8461 gcggcgggcg tctgatttat ctcggtgcgg gaacatccgg tcgtctgggt attcttgatg
     8521 ccagtgaatg tccgccgacc tacggcgtga aaccgggcct ggtagttggt ttgattgctg
     8581 gcggcgaata tgccattcag cacgcggtgg aaggcgcgga agatagccgg gaaggcggcg
     8641 ttaacgatct gaaaaatatt aatttaacgg cacaggatgt ggtggtcggt attgccgcca
     8701 gcggtcgtac gccgtatgtg attgccggac tggaatacgc ccgccagctc ggttgccgca
     8761 cagtgggaat ttcctgtaat cccgggagcg ccgtttcaac caccgctgag tttgccatta
     8821 cgccagttgt tggggccgaa gtggtcacag gttcttcgcg aatgaaagca ggtacggcgc
     8881 agaaactggt gctcaatatg ctctccaccg ggctgatgat taaatccggc aaagtgttcg
     8941 gcaacctgat ggtcgatgtg gtcgccacca acgaaaaatt gcatgtgcgc caggtcaata
     9001 tcgtcaaaaa cgctaccgga tgtagcgcag agcaagcgga agcggcattg gttgcctgcg
     9061 agcgcaactg taaaacggcc attgtgatgg tgctgaaaaa tctcgatgcc gcagaagcta
     9121 aaaaacgcct tgatcaacac ggcggcttta ttcgtcaggt tttagacaag gaataaccca
     9181 tggccaaaga gatcagcagt gaacttctga acaccattct tacccgtgtc ggcggaccgg
     9241 gaaatatcgc cagttgtggt aactgtatga cgcgcctgcg tctgggtgta catgacagtt
     9301 cactggttga tcccaatatc aaaacgctgg aaggcgtgaa gggcgtcatt ttgaccagcg
     9361 atcaggtgca ggtggttttt gggccgggca aagcgcatcg tgccgcaaaa gccatgagcg
     9421 agctgctggg agaagcaccg gtacaggatg ccgcagaaat cgccgcccag aacaaacgtc
     9481 agttaaaagc caaacaaacc tccggcgtgc aacaatttct cgccaaattc gccaccatct
     9541 tcacgccgct gatccccggt tttattgccg ccggtctgct gctggggata gcgacgttaa
     9601 ttgccacggt gatgcacgtt ccggcagatg ctcagggcac gctacccgac gcgctgaatt
     9661 ttatgaaggt gttcagcaaa ggtttgttca ccttcctggt gattctggtg ggctataacg
     9721 ccgcccaggc atttggcggc acgggcgtaa atggcgcgat tatcgccgcg ctctttttgc
     9781 tcggttataa cccaactgca accaccggtt actacgccgg ttttcacgat ttctttggtc
     9841 tgcccatcga tccgcgcggc aatattatcg gcgtgttgat tgccgcgtgg gcctgcgccc
     9901 gcatcgaagg catggtgcgc cgctttatgc cggacgatct cgacatgctg ttgacctcgt
     9961 taatcaccct gctgatcacc gctacgctgg cctacctgat cattatgccg ctgggcggct
    10021 ggctgtttga aggcatgtcg tggctgttta tgcacctgaa cagtaatccg ctcggttgtg
    10081 cggttttagc cgggctgttc ctgatcgccg tggtgtttgg cgtgcatcag ggctttattc
    10141 ctgtttacct cgcgttaatg gacagtcagg gattcaacag cttatttccc atcctttcga
    10201 tggcaggcgc gggccaggtg ggcgcggcgc tggcactcta ctggcgggcg caaccgcaca
    10261 gtgcattacg cagtcaggta cgcggggcga ttattcccgg cctgctgggc gttggcgaac
    10321 cgttgattta tggcgtcacc ctgccccgca tgaaaccgtt tattaccgcc tgtttaggcg
    10381 gcgcggcggg cggtttgttt atcggattga tagcctggtg gggtctgccg atgggcttaa
    10441 acagcgcctt tgggccgtct ggtctggtgg cgctgccgct gatgacttcc gcacaaggca
    10501 tccttccggc aatggcggtt tatgcaggcg ggattctggt ggcatgggtt tgcgggttta
    10561 ttttcaccac gctctttggc tgccgtaacg tcaatctgga ctgataatat gaaacggaca
    10621 atgctctatc tttctctgct ggctgtcagc tgtagcgtca gcgccgcaaa ataccctgtt
    10681 ctgacagaaa gttcgccaga gaaagcaggg ttcaacgtcg aacggcttaa ccagatggat
    10741 cgctggataa gccagcaggt ggatgccggt tatcccggcg taaacctgct gattattaaa
    10801 gataatcaga ttgtgtatcg caaagcctgg ggagcggcga aaaagtacga tggcagcgta
    10861 ttgatggaac agcctgtcaa agccaccacc gggacgctgt atgacctggc ttccaatacc
    10921 aagatgtacg ccactaactt tgctctgcaa aagctaatga gcgaaggcaa gctgcatcct
    10981 gacgatcgga ttgcgaaata tattccggga tttgccgata gtccaaatga caccatcaaa
    11041 ggcaaaaaca cgctgcggat ttctgacctg ctgcatcaca gcggcggttt cccggcggac
    11101 ccacaatatc cgaataaagc ggtcgcgggc gcgttatatt cccaggataa aggccagacg
    11161 ctggagatga tcaagcgcac gccgctggag tatcagcccg gcagcaagca tatctacagc
    11221 gatgtcgatt atatgctgct tggatttatc gtcgagtcgg ttaccggtca gccacttgac
    11281 cgctatgttg aagagtcgat ttatcgcccg ctcggcctga cgcatacggt gtttaatccg
    11341 ctactgaaag gctttaaacc gcaacaaatt gccgccacgg aactcaacgg caatacccgc
    11401 gatggcgtga tccatttccc ggatatccgc acctccactc tctggggtca ggtgcacgat
    11461 gaaaaagcct tttattcgat gggcggcgtt tccgggcatg cagggttgtt ttccaatacc
    11521 ggcgatattg cggtgttaat gcaaacgatg ctgaacggcg gcggctatgg cgatgtgcag
    11581 ttgttcaatg cagaaacggt gaagatgttc accaccagct ctaaggaaga tgccactttt
    11641 ggccttggct ggcgcgtgaa tggtaatgcc accatgacgc cgacgtttgg cacgctggca
    11701 agcccgcaaa cctacggtca cactggctgg accgggacgg tgaccgttat cgatccggtg
    11761 aatcatatgg cgattgtgat gttaagcaac aagccacatt cgccagttgc cgatccacaa
    11821 aaaaatccca atatgttcga aagcggtcag ctaccgattg caacttatgg ttgggtagtt
    11881 gatcaggtgt atgcggcgtt aaagcagt
//
LOCUS       DAPOZO010000132        11876 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPOZO010000132 DAPOZO010000000
VERSION     DAPOZO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.132"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(50..1390)
                     /gene="lamB"
                     /locus_tag="ROI21_004193"
     CDS             complement(50..1390)
                     /gene="lamB"
                     /locus_tag="ROI21_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDX5113046.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(1462..2577)
                     /gene="malK"
                     /locus_tag="ROI21_004194"
     CDS             complement(1462..2577)
                     /gene="malK"
                     /locus_tag="ROI21_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDX5113047.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            2942..4132
                     /gene="malE"
                     /locus_tag="ROI21_004195"
     CDS             2942..4132
                     /gene="malE"
                     /locus_tag="ROI21_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDX5113048.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEIPPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGHPSKPFVGVLSAGINAASPNKELAKEFLENYLLTNEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4286..5830
                     /gene="malF"
                     /locus_tag="ROI21_004196"
     CDS             4286..5830
                     /gene="malF"
                     /locus_tag="ROI21_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDX5113049.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            5845..6735
                     /gene="malG"
                     /locus_tag="ROI21_004197"
     CDS             5845..6735
                     /gene="malG"
                     /locus_tag="ROI21_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDX5113050.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(6829..7239)
                     /gene="psiE"
                     /locus_tag="ROI21_004198"
     CDS             complement(6829..7239)
                     /gene="psiE"
                     /locus_tag="ROI21_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDX5113051.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            7454..7732
                     /locus_tag="ROI21_004199"
     CDS             7454..7732
                     /locus_tag="ROI21_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113052.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(7779..9875)
                     /locus_tag="ROI21_004200"
     CDS             complement(7779..9875)
                     /locus_tag="ROI21_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDX5113053.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGVDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(9875..10612)
                     /locus_tag="ROI21_004201"
     CDS             complement(9875..10612)
                     /locus_tag="ROI21_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDX5113054.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSKTPDALNADILQTLTQRIPQ"
     gene            complement(10609..11247)
                     /locus_tag="ROI21_004202"
     CDS             complement(10609..11247)
                     /locus_tag="ROI21_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDX5113055.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDES
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(11434..11676)
                     /locus_tag="ROI21_004203"
     CDS             complement(11434..11676)
                     /locus_tag="ROI21_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113056.1"
                     /translation="MKKVLYGIFAISVLAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
BASE COUNT         2774 a         3190 c         3067 g         2845 t
ORIGIN      
        1 ttggttgccg gatgcggcgc aagcacctta tccggcccag gttttgctat taccaccaga
       61 tttccatctg ggcaccgaag gtccactcgt cgctgtcacc acggccgaag ctgccgccgt
      121 tgaaatcagc aggaacggct ttgccgaagt tagtgttggt attggcgtta ccggtgtagt
      181 cataacccca tttctcatcc cacttggcgt aggttgcgaa gacacgaata gccgggcgtg
      241 accagatact gtcgccagcc tgccattgtt gtgcgagggt aattttgtac tgattgttct
      301 tgtcgccggt gcgctgggat tcgacgttgt cgtagccgat ttccatcacg gtgctcatga
      361 ttggcgtcca cttgtacatc gggcgaatac cgacggtcca ccacttggtg ccgttgtcgt
      421 tatcccagtt gatatcctgg tacataccca cgtacatcat gtcccagtta tcgcccatgg
      481 agatcgcacc gtggtcgagg atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa
      541 atttttcgtt atcaaacgcg acgccagaac cctgcgacag acctttaccc tgcgaggtca
      601 tcgagtcagt agcgtactga acaacaaact tgttaaagcc cttcaggaca ctctgagtat
      661 gttcagcagt gaataaccaa ccgtctttcg atgcgccatc aaccagacga tagttatcac
      721 gcaggttggc acgaccgtag tcgacaccca gttctaatgt gccgcccggg ttgatttcca
      781 tctgcgctaa acgcacgtcg aaaacgtcgt tcgcggtttc gttggtatag tcataaatat
      841 tgttgctggc gaaagaggaa gaaccacctg cttcagagga gcgggttgct gccagagaga
      901 gtttaccgaa gccaacatcg atgttttcca gaccggcacc aggaccagaa atatcccagt
      961 agtagaagtc gatcatatga acgtcatgac gttggtagaa gcgcttacct gcccagatgg
     1021 tggaacctgg cagccattcg atcaggtttt taccctgtac gtttgcttca cggaaggccg
     1081 gatcggtagc ttcccagtca ttctgttgtg cgacggaata ggccacgtta gtgtcgaaat
     1141 agaagctctt atcgccctct ttccacactt cctgacccaa ttttaattca gcataagttt
     1201 cacattcgtt gccaagacgg tatttacttt gagcaccggt agtctggaaa cactgttgtt
     1261 caccgccgct acctgtccag ccaataccgg aacgtgcata gccgtggaaa tcaacagcca
     1321 ttgcctgagc agacattacg cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag
     1381 taatcatcat tctatctcct gagtcattgc ttttcttttt tcacatcacc tgtgacaggc
     1441 tttgtgtgtt ttgtggggtg cttaaacgcc cggctcctta tgcagtcgac gacatgcagt
     1501 gccatcctca cggaacagat ggcaacgctc tggcggcagg ccgatagcga atgtggcacc
     1561 ttcttctacc aacaccacgt cgttctggcg gtacaccagg ttttgacgaa tggaagggat
     1621 ctggatatgg atttgggttt cgttgccgag ttgctcgacg acctgaactt caccctcaag
     1681 gatgacgtca gcgatatcac tcggcagtag atgttccggg cgaataccca gtgacatatt
     1741 ggctccaacc tggacatcac ggctttcaac aggcagccag acttgctgac gatttggcat
     1801 cggcagctcc acctgcactt gatcgattgc ggtggcagtc acttttaccg gcaggaagtt
     1861 catctttggc gaaccgataa atccggcgac aaaacggtct gccggatagt ggtacagctc
     1921 cagcggtttc ccaacctgcg ccacgcgacc ggcgtccagc accacgattt tgtcggctag
     1981 tgtcatcgct tcgacctgat cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg
     2041 cagacgggag atttcgatac gcatttgcac acgcagtgca gcatcaaggt tggagagcgg
     2101 ttcatcgagc aaaaatacgc ttggctcggc caccagcgta cggccaatcg ccacacgctg
     2161 acgctgacca ccggagagcg ctttcggttt gcgatccagc aaatgcgcca gttgtagcac
     2221 ttccgccacc tggttgacgc gttggttaat cacctctttt tttgcgcctg ccagtttcag
     2281 gccaaatgac atgttttctg ctactgacag gtggggatag agcgcgtaag actgaaacac
     2341 cataccaacg ccgcgttctg ctggcggagt gtcattcatc cgtttctcac cgatgaacag
     2401 gtcgccgctg gtgatcgtct caagcccggc aatcatgcgc agtaaagtcg atttaccgca
     2461 gccagacggt ccgacaaaca ccacgaattc accttcatgg atatcgagat tgatatcttt
     2521 cgataccacg acctcgcccc aggctttcgt tacattttgc agctgtacgc tcgccatgcc
     2581 cttctccctt tgtaacaacc tgtcatcgac agcaacattc atgatgggct gactatgcgt
     2641 catcaggaga tggcttaaat cctccacccc ctggcttttt tatgggggag gaggcgggag
     2701 gatgagaaca cggcttctgt gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc
     2761 ttgcaaaaat cgtggcgatt ttatgtgcgc atctccacat taccgccaat tctgtaacag
     2821 agatcacaca aagcaacggt ggggcgtagg ggcaaggagg atggaaagag gttgctgtat
     2881 aaagaaacta gagtccgttt aggtgttttc acgagcactt caccaacaag gaccatagat
     2941 tatgaaaata aaaacaggtg cacgcatcct cgcattatcc gcattaacga cgatgatgtt
     3001 ttccgcctcg gctctcgcca aaatcgaaga aggtaaactg gtaatctgga ttaacggcga
     3061 taaaggctat aacggtctcg ctgaagtcgg taagaaattc gagaaagata ccggaattaa
     3121 agtcaccgtt gagcatccgg ataaactgga agagaaattc cctcaggttg cggcaactgg
     3181 cgatggccct gacattatct tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg
     3241 cctgttggct gaaatccccc cggacaaagc gttccaggac aagctgtatc cgtttacctg
     3301 ggatgccgta cgttacaacg gcaagctgat tgcttacccg atcgctgttg aagcgttatc
     3361 gctgatttat aacaaagatc tgctgccgaa cccgccaaaa acctgggaag agatcccggc
     3421 gctggataaa gaactgaaag cgaaaggcaa gagcgcgctg atgttcaacc tgcaagaacc
     3481 gtacttcacc tggccgctga ttgctgctga cgggggttat gcgttcaagt atgaaaacgg
     3541 caagtacgac attaaagacg tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt
     3601 cctggttgac ctgattaaaa acaaacacat gaatgcagac accgattact ccatcgcaga
     3661 agctgccttt aataaaggcg aaacagcgat gaccatcaac ggcccgtggg catggtccaa
     3721 catcgacacc agcaaagtga attatggtgt aacggtactg ccgaccttca agggtcatcc
     3781 atccaaaccg ttcgttggcg tgctgagcgc aggtattaac gccgccagtc cgaacaaaga
     3841 gctggcgaaa gagttccttg aaaactatct gctgactaac gaaggtctgg aagcggttaa
     3901 taaagataaa ccgctgggtg ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga
     3961 tccacgcatt gctgccacca tggaaaacgc ccagaaaggt gaaatcatgc caaacatccc
     4021 gcagatgtcc gctttctggt atgccgtacg tactgcggtg atcaacgccg ccagcggtcg
     4081 tcagactgtc gatgaagccc tgaaagacgc gcagactcgt atcaccaagt aatgctgtga
     4141 aatgccggat gcggcgtgaa cgccttgtcc ggcctacaaa accgaaacgt atgtaggcct
     4201 ggtaaggcgc gtcagcgtcg catcaggcag ttgttgtcgg ataaggcgtg aaagccttat
     4261 ccgtcctgga atgaggaaga accccatgga tgtcattaaa aagaaacatt ggtggcaaag
     4321 cgacgcgctg aaatggtcag tgctaggtct gctcggcctg ctggtgggtt accttgttgt
     4381 tttaatgtac gcacaagggg aatacctgtt cgccattacc acgctgatat tgagttcagc
     4441 ggggctgtat attttcgcca atcgtaaagc ctacgcctgg cgctatgttt acccgggaat
     4501 ggctggaatg ggattattcg tcctcttccc tttggtctgc accatcgcca ttgccttcac
     4561 caactacagc agcactaacc agctgacttt tgaacgtgcg caggaagtgt tgttagatcg
     4621 ctcctggcaa gcaggcaaaa cctataattt tggtctttac ccggcgggcg atgagtggca
     4681 actggcgctc agcgacggcg aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg
     4741 cggcgagcaa aaactgcaac tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa
     4801 tctgcgcgtg attacccaga atcgtcaggc gctgagtgac attaccgcca ttctgccaga
     4861 tggcaacaaa gtgatgatga gctccctgcg ccagttttct ggcacgcagc cgctctacac
     4921 actcgacggt gacggcacgt tgacgaataa tcagagcggc gtgaaatatc gtccgaataa
     4981 ccaaattggc ttttaccagt ccataaccgc cgacggcaac tggggcgatg aaaagctaag
     5041 ccccggttac accgtgacca ctggctggaa aaactttacc cgcgtcttca ccgacgaagg
     5101 cattcagaaa ccgttcctcg ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt
     5161 ctttttaacg gtggcggtcg gcatggttct ggcgtgtctg gtgcagtggg aagcgttgcg
     5221 cggtaaagcg gtctatcgcg tcctgctgat tctgccctac gcggtgccat cgttcatttc
     5281 aatcttgatt ttcaaagggt tgtttaacca gagcttcggt gagatcaaca tgatgttgag
     5341 cgcgctgttt ggcgtgaagc ccgcctggtt cagcgatccg accaccgccc gcacgatgct
     5401 gattatcgtc aacacctggc tgggttatcc gtacatgatg atcctctgca tgggcttgct
     5461 gaaagcgatt ccggacgatt tgtatgaagc ctcagcaatg gatggcgcag gtccattcca
     5521 gaacttcttt aagattacgc tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc
     5581 cagcttcgcc tttaacttta acaacttcgt gctgattcaa ctgttaacca acggcggtcc
     5641 ggatcgtctt ggcacgacca cgccagccgg ttataccgac ctgcttgtta actacaccta
     5701 ccgcatcgct tttgaaggcg gcgggggtca ggacttcggt ctggcagcag caattgccac
     5761 gctgatcttc ctgctggtgg gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa
     5821 gtttgattaa gggagataac aaaaatggca atggtccaac cgaaatcgca aaaagcacgt
     5881 ttgtttatta ctcacctgct actgctactt tttatcgcgg cgattatgtt cccgctgctg
     5941 atggtcgtcg ctatctcgct gcgtcaggga aacttcgcga ctggcagcct gatcccggag
     6001 caaatctcct gggatcactg gaaactggcg ttaggtttta gcgtggaaca ggctgatggt
     6061 cgcattacgc caccgccatt cccggtactg ctgtggctgt ggaactcggt aaaggtcgcc
     6121 gggatttccg cgattggcat tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt
     6181 atgcgctttc caggcaaagc gacgctgctg aaaggaatgc tgattttcca gatgttcccg
     6241 gcagtacttt cactggtcgc gttgtatgcg ttgtttgatc gtctgggtga gtacattcca
     6301 ttcattggcc tgaatactca cggcggcgta attttcgcgt atctgggtgg gattgcgctg
     6361 catgtctgga ccatcaaagg ctatttcgaa accatcgaca gttcgctgga agaagcggcg
     6421 gcgctggatg gtgcgacacc gtggcaagcc ttccgccttg tcttgttacc gctgtcagta
     6481 ccgattctgg cggtggtgtt catcctgtcg tttatcgctg ccattactga agttccggtc
     6541 gcgtcgctgt tactgcgtga cgtaaatagc tacaccctgg ccgtggggat gcagcaatac
     6601 ctcaacccgc aaaactacct gtggggtgac tttgctgccg ctgccgtgat gtctgcatta
     6661 ccgatcacca tcgtcttctt gctggctcaa cgctggctgg tcaacggcct gacggcaggt
     6721 ggtgtgaaag gttaaagatg ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg
     6781 ttatcccaac ttgtgactgt tattcggcgc tccacggagc gccttttttt actcccgctt
     6841 cagccgtttc gaattacaca gccacagggt gatcaccagc aacaggatcg ctgccgagta
     6901 gatcaacaca tccagtggcg atttatgatc gacgatgatc aagcgcacaa tggcggtgat
     6961 cccaatatag acaaagtaac gtagcgggaa gtgaaaaccg gactgaaagt acttcacaat
     7021 cagcgcgata aattcgaaat agagaaagta aaccaccagt ccttctacca gctcatattt
     7081 gctggtttgt tctggcgcga acagcacatc agccagatgc accgtttctt tgccgaggaa
     7141 gacaaccaaa atcaggccga ggcacaacag gccaagattg agtacggtct gcaaaatggt
     7201 ggagataaac tccacgcgcg gacgagagag tgacgtcata gcagtacctc cttcacagtg
     7261 gcgagcttcc tgtataacgc aaaaatgtga cggagatcta tattttttgt gtatgttttg
     7321 ttcagctcga ttaagtgggc atctcccatc aacgtctcat ctgacaaaac acacagccca
     7381 aaaaaatcac ccattcaggt gattcccagc caccagcaat gcgcgaaaaa tcgacaatgt
     7441 tcctctggaa caaatgaaac gtaacctgat aaaggtggta aaaatgaagc cttattttgc
     7501 tgctttgatg ttatcagtct ctgttcttcc tgcttatgcc ggtccgctcg gtactgctga
     7561 taaagccgac ctaccgcaaa gcaatgtctc cagcccaatg atggcccagt cgctcaggca
     7621 accagacctg caaccaatat cgacggacag gaagacagaa tgttttcgcc tttacacacc
     7681 agatcgcaaa ccgggggtga actgcgttcc tgatggctcg acaggccatt aagaatgagg
     7741 gtgccagtga gtgcgaaaac tggcaccttt tccatgactt agcggaaatt gacgctacgg
     7801 tcgctggtca tgtcatacaa ctggaactta cgcccaagtt gctgaccacc gtcacgcgtc
     7861 agcggcgtcc agccaattgc cgcacggctg cgtgtcgggc cagacgagaa gagatccagc
     7921 ggtacagaga catacacgcc tttggtgaag tccccttcgc cgtactcctc tttcgaaaca
     7981 ttcgtgatcg tggcatagcc acccaccacc acgccgctat caaagcgttt ggcgatctcc
     8041 agcgtgccgc ctttatcccc agccagatac tgcccgacgc tcgctttcac taacacatcc
     8101 tgagcgaaag atggcgtcca gtaggcggtc agatgtccgg ttttcacgct gtagtcggtg
     8161 aatttcatca tatcttttgc actacgccag tcgcgctgtt taacgtagtt ggcatccaca
     8221 ccaaacgccc agttgctgtc cagcgggcga tacaacactt ctgcccccgc accgccaaac
     8281 atggtttcga gataacctcc gtagacctgg ccgtagaagc cgttgcccag atgctggaag
     8341 tagttggctt gcaggttatt cacatagaca tcgttctgca catactcgcg cacatgggta
     8401 cgaacgcgcg gtaagtgcga gtcctgcggt ggattagtgt agttaaactt gtcgtagttg
     8461 ttggcaaggt tagcgaacag gctgccggtg gtcagcagat ggtccgtcag ccacaaatct
     8521 gccgttccca tcacgcccag ctgatacatg taaaagtttt ccgggccacc gaccgactgg
     8581 ttcaacaccg gatcgatatg gaaatcaaag cgcgatttgt cgatatacca gccctgctcg
     8641 gtggactgcg gaaccactgg ctcgacgcgt ttttgcgcca gcgtcgtttc gtggcccaac
     8701 ggctctccgg caagatgatt tttcaggctg gcgacatcgg tttccgtcgt cacctgcggc
     8761 atgttaaggc gattctccgt aatgcggatc gttttgatcc catccggcag atcgttcatc
     8821 acgatccgat tagcacggat gatcccttcg cgcgaatcac gatatttcac ctgctcgccg
     8881 gtaacataca gagtatcgcc tttcgcctgg atctgtggat cggcaagtcc ggcattgtat
     8941 ttcaacagcg ttaactgatt cgccaccacc gaatgctgca aaatggcatc ctgcggctgc
     9001 ggttgatatt gcgggcgggc gttatcgttg taagacgggc gcagatcgtt aaagttggtg
     9061 cgcaacgtaa cgccaaacat aaaggtgttg ccacgttcat agctaaggtt aacgtcggcc
     9121 caatcggtaa cgcgataaat cgcaccgacg ttaaacttac ttttttgctc cagcttcccg
     9181 gcaaaatcct gctgataatt attgccttca tactccagtt tcaggcgcag cggttgccag
     9241 ggcgtctggt attccacgcc gccaaacagt gaggcaggac cgtggaacat ctggctaccg
     9301 tcgatagatc ccgcctgttt gtagctgtta tcgcgatagc aatatttgtc actggctgaa
     9361 cagagcggat ttttcacgtt accgctggtg cccagatatc cccatcccag gccgagcgtg
     9421 aaatcgaacg gcccccaggc tttgctggca acaagatatt ccgcatcaaa cagccccgta
     9481 ccgccgatat cccgcgcacc aaccgccact tgcggcagcc aataactctc ttcccacaaa
     9541 cgcagtttga gatcgaaggc tttatcttta tacgtttgat caccagagaa cgcttcgacg
     9601 ctgctgtact gccgggtgcg cacgtcggtg tagcgcagcg ttgtttccag ccacgggaag
     9661 agttgcactg aagctgagta ataacggtac tgatcgttat cgcgatagtt gaggctcaac
     9721 tccccttccc gcgccatgcg cgcggtgggc gtttgtaata atcctacgcc accgaaatcc
     9781 gactgcgacg gaccaatggg cgcaggatat gtttcgccgt agcaagctgt gctaatgccc
     9841 agcgccagta agctaagcag atgtcttttt ttcattattg aggtatccgc tgcgtcagag
     9901 tctgaagaat gtcggcgttc agggcatcag gcgtcttact ccagacggag tccgcgaggc
     9961 caacataaat aatgctgccg ggcatcggct ctacgtgacg cttgttccag taagccaccg
    10021 gcgctttttg cgtgcgtccg tccgggtaaa ccacccacgc gtagctgcga tccgcaccgc
    10081 taagcaggtt ctgcccggag agatagctcg ccacgtcgcg acctggagtg aaaggctgct
    10141 tgccaggacg gctgataagc ccgaacaacg tgaccgtgga cggcggtggt ccgacccaca
    10201 gcgtgtagtt gccctgcaac ggtgggttag cgcgttcggc aacgcggacg atatcgggat
    10261 caagattgat tttttgcctg cccgtcacct tcaacgcctg aatttgctgg cgtaaggcgt
    10321 taatggcggc ggcatcgtca gcgctggaat ctgcggctag ttctgccagc cgtgtcagca
    10381 acgcctgctg ctgacgcaat gctgccgccg ttgccagctc ttcgctaatc accgcaccgg
    10441 gccaccaact gtttgccagc cgtggttggc ctaccagatc gattaaatgc tctgcccccg
    10501 tcagcgtttt ggcctcactg ctgccattgc tgaatacctt gacggttccc gccgcgaaga
    10561 ccgatgacgc ccccacactc aaaatcaatg cgacaatagt ttgtttaatc attgttttgc
    10621 cgccttgatg agagtggttt tcaccgggaa ataatccgcg ccgagatact gttccgactg
    10681 gcgaatttgc ccttcgctgt cgatccaata gcggttatgc cagcgcgcct ggtcggtgga
    10741 gacttcttcg tccagcacgc gaaccgggga ttcatcgctg ccgactttga cggtatcggt
    10801 gccatcccat ttgaagactg agcgtgcggt ggcgtagcgt acctgctggt actcggtcca
    10861 gcccatcgtg cgcgtccagg ttgcaccatc aacaatttgt gcgggtttaa tcagcgggtc
    10921 ggcagcaagg ttattcactt caatcaggtt gtcgccgcca agcaaagtct tcaccagacg
    10981 accatgttgt gtgacgagag tggcctgatc ctgagtaacc catttttgtt gcccatcctc
    11041 ggcaaaggcc agcactacaa acaactgcgg cccgccatta agctgcatat actggctggc
    11101 gtagggcata ttttgaatat catcatccgt cagctgtacg cctggtgtac cgaacagact
    11161 gtcccacagt gaattgccca gctctttagt cgtggctgaa caggcctgta atagcaggca
    11221 gataagaatg agtgcaggtc gcttcacgac tcttctccag aggggcagaa taaccacacc
    11281 ggagtgtggt tatggttaat ccccttcata cttcaagctg cagatgcggt gaccgcactc
    11341 gttcaccccg gtcacatagt tatctatgct cccggggact cactcgcttg tcgccttcct
    11401 gcaactcgaa ttattttggg gatacatact ttattactgg gtactggtgg tcgtggttgt
    11461 tgtggtcccg gtattagaac catcaccgcc accggttgcc gcgagcgcga cacccaccgc
    11521 cgagcttacg gtgctgacgc tggtcgcgga ggaactcccc gccgaaaccg acgttgctgc
    11581 cgaccctgct gcttcgccca cctgtacagg tgcagcccac gcagaagtcg ccgcaagcac
    11641 agatatggca aaaatgccat acagaacttt tttcataaca atttcccttc attgaatgaa
    11701 tggagattta cccaaaaaca tttcgggcgg gatcgagtat acacaactaa agcatgcgaa
    11761 ttacggtgtg gggaattagc gatggggttt taggataatt ggaatgaata tcaaatataa
    11821 gtttcaactt atatataaat aagattaaaa atcaaaaagt taaaatgaaa tcaaaa
//
LOCUS       DAPOZO010000133        11625 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPOZO010000133 DAPOZO010000000
VERSION     DAPOZO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.133"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            161..334
                     /locus_tag="ROI21_004204"
     CDS             161..334
                     /locus_tag="ROI21_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113057.1"
                     /translation="MKKLALILVIGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(406..555)
                     /gene="hokB"
                     /locus_tag="ROI21_004205"
     CDS             complement(406..555)
                     /gene="hokB"
                     /locus_tag="ROI21_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDX5113058.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            954..2594
                     /gene="trg"
                     /locus_tag="ROI21_004206"
     CDS             954..2594
                     /gene="trg"
                     /locus_tag="ROI21_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDX5113059.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAIKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FIVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITSVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVAMAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2632..3555)
                     /locus_tag="ROI21_004207"
     CDS             complement(2632..3555)
                     /locus_tag="ROI21_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5113060.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3772..5115
                     /locus_tag="ROI21_004208"
     CDS             3772..5115
                     /locus_tag="ROI21_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDX5113061.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAEEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHSGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5340..6995
                     /gene="opgD"
                     /locus_tag="ROI21_004209"
                     /gene_synonym="mdoD"
     CDS             5340..6995
                     /gene="opgD"
                     /locus_tag="ROI21_004209"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDX5113062.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVDGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            7135..7359
                     /locus_tag="ROI21_004210"
     CDS             7135..7359
                     /locus_tag="ROI21_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDX5113063.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            7422..7961
                     /gene="rimL"
                     /locus_tag="ROI21_004211"
     CDS             7422..7961
                     /gene="rimL"
                     /locus_tag="ROI21_004211"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDX5113064.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNVQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(7953..8933)
                     /locus_tag="ROI21_004212"
     CDS             complement(7953..8933)
                     /locus_tag="ROI21_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDX5113065.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQDAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIEKHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            9057..10049
                     /gene="tehA"
                     /locus_tag="ROI21_004213"
     CDS             9057..10049
                     /gene="tehA"
                     /locus_tag="ROI21_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDX5113066.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            10046..10639
                     /gene="tehB"
                     /locus_tag="ROI21_004214"
     CDS             10046..10639
                     /gene="tehB"
                     /locus_tag="ROI21_004214"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDX5113067.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10942..11610
                     /locus_tag="ROI21_004215"
     CDS             10942..11610
                     /locus_tag="ROI21_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDX5113068.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPHSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT         3008 a         2705 c         2912 g         3000 t
ORIGIN      
        1 atttatttgt atgacaaata aaaccataat ccttatccat acgtccatct ggcttatctt
       61 taatcaattc acccgatctt tgatctcatc aacggtatca aaataaacca gcgtataacg
      121 ttcaaatcga acataagatt cgataaacca tggaggttat atgaaaaaat tggcactcat
      181 cttggttata ggagcgcttg tttcctttta tgccgatgcc gggcgcaaac cctgttctgg
      241 ttcgaaaggg gggatctcac actgtacggc aggcggcaaa tttgtctgta atgatggttc
      301 tattagtgca tcgaaaaaaa catgcactaa ctgaagtgta aaaggggtgc catgagaaaa
      361 tgaatctgct agtcagatgc gcggggaaaa tccccgcgct tgcccttacc tggacgtgca
      421 ggccatgagc gcagcaacct ccttatcacc gtcccggaac cgcagttcgt agagcgtttg
      481 tcgggtcagg agtgtgaatg tcagaatcgt aatgcagata atgagcagac acaccaccag
      541 agggttgcgc ttcatagcct ttctccttgc cggatggcgg gtaagaggct aacatctgaa
      601 ttgcttggtt cattcgttgg cctcggttga tagaaatatc ggtcggggcc ttcgtctttc
      661 tgattcccgg ttagcctgaa aacagaaagt ctcaggcacc cgtgagcatc ctatgtggtt
      721 tccttaggga cgaaaataat cacctgacga aattgcgtgc tgttttccag aatttttcgt
      781 cattcgggtt agccagttta gccattcgtt actctcttca ttccaatagc attaattttc
      841 tctgcaataa ttgttgtaaa aatgtgacgc aaagaggttt ttggtcataa gtaattatcg
      901 tcaagtgccg atgactttct atcaggagta aacctggacg agagacaacg gtaatgaata
      961 caactccctc acagcgatta ggttttttgc atcacatcag gctggttccg ttatttgcct
     1021 gcattcttgg cggtatctta gttctattcg cattaagttc agctctggct ggctatttcc
     1081 tctggcaggc cgatcgcgat cagcgtgatg ttactgcgga gattgagatt cggaccgggt
     1141 tagcgaacag ttcagatttt ttgcgttcag cccggatcaa tatgattcag gctggtgctg
     1201 cgagtcgtat tgctgaaatg gaagcaatga agcgaaatat tgcgcaagcc gaatcggaga
     1261 ttaaacagtc gcagcaaggt tatcgtgctt atcagaatcg gtcggtgaaa acacctgctg
     1321 atgaagccct tgacactgaa ttaaatcaac gcttccaggc ttatatcacg ggtatgcaac
     1381 cgatgttgaa atatgccaaa aatggcatgt ttgaagcgat tatcaatcat gaaagtgagc
     1441 agatccgacc gctggataat gcttataccg atattttgag caaagccatt aagatacgta
     1501 gcaccagagc caaccaactg gcggaactgg cccatcagcg cacccgcctg ggtgggatgt
     1561 tcatgattgg cgcgtttgtg cttgccctgg tcatgacgct gataacattt atagtgctac
     1621 gtcggatcgt cattcgtcca ctgcaacatg ccgcacaacg aattgaaaaa atcgccagtg
     1681 gcgatctgac gatgaatgat gaaccggcgg gtcgtaatga aatcggtcgc ttaagtcgtc
     1741 atttacagca aatgcagcat tcactgggga tgacagtagg gaccgttcga cagggcgcgg
     1801 aagagattta tcgtggcacc agcgaaattt ccgctggcaa tgcggacctt tcatctcgca
     1861 ctgaagaaca agcggcggca attgaacaaa ctgccgccag catggagcaa ctcactgcga
     1921 cggtgaaaca gaatgcggat aacgcgcatc atgccagcaa actggcgcag gaggcttcta
     1981 ttaaagccag cgatggcggg cagacggttt ccggtgtagt aaaaacgatg ggcgctatct
     2041 ccacgagttc gaagaaaatt tccgagatca cttccgtcat caacagtatt gctttccaga
     2101 cgaatattct ggcactgaat gctgccgttg aagccgcgcg agcgggtgag caaggacgtg
     2161 gatttgccgt tgtcgccagc gaagtacgga cactcgcaag ccgcagcgcc caggcggcga
     2221 aagagattga aggcttgatc agtgaatctg tcaggttaat tgacctgggg tcggatgagg
     2281 tggcaatggc agggaaaacc atgagcacta ttgttgatgc cgtcgcgagt gtcacacata
     2341 tcatgcagga aatcgccgcc gcctcggatg aacaaagtag aggcataact caggttagcc
     2401 aggcgatttc tgaaatggat aaggtgacgc aacagaatgc ttctctggta gaagaggcct
     2461 cagcggcggc ggtgtccctt gaagaacagg ctgcacgatt aactgaggcg gtggacgtat
     2521 tccgtctgaa caaacattct gtgtcggcag aacctcgcgg agcgggtgaa ccagttagtt
     2581 tcgctacagt gtgaaaatgt tcaaggaggg atcgacagat cccttcacct ttcagaacgg
     2641 cattgatttt cgaatagcgt taatcatcaa ctggcaacca gaagagaacg tcgcatctac
     2701 gcgggtcagt attccaatcg gttcgcctgc accatgccct ggaacaggca gggccaccag
     2761 cgtggcatga cgcaggtcgt cttttacagc gccagaaggg acaaaccaca cgtagtcgta
     2821 ttcaaccgta agttgacgag atagcgaagc agacagcgtt tcgatacaac ccgaaggaat
     2881 tttacagccc tggctctgca ctaatgcatc tgaatgctgg cgtggcgcag tgccttctgg
     2941 tgatacaacg accggccatt ccagcacccg gcttagcgtt acgttctcct gaagtagcgg
     3001 gtgattaggg cggacgacca gcttcagcga ttcaagaaac agcagttcgt aattaagtcc
     3061 ggtcatcagt tcaggatctg acatccgacc aatgccgata tcgatttccc cggtttttaa
     3121 acccgcaaga atcatagggt tactcattgt cgcaacttgc aaggtcgtct ctttttgttg
     3181 ctgatgaaac tgacctataa ccgaaggtaa tatccccagt gccgcagtag gtagtgcacc
     3241 aaccctgacg acatcattat taagaccttc tttacgatga agcgactgtc cggcagtgtt
     3301 gatggcgtca agaactctga ctgcatgcgt taaaaattgt tcgccgggta aggtaagttg
     3361 cgccccctga cgaccacgct caaacaagcg agcgcccgtc agttgctcca gttcattcag
     3421 tgttttagag agcgcaggtt gactcaaatt aagggtttca gccgcgcgcc ccaaagttcc
     3481 ttgttgtgcg acagctacga atgtatgaag gtggcgcaaa cggatgcgct gactaaacag
     3541 actatttttt tccataagcg atgttaaaaa cgaagcggtg tcgctgacaa gtgaagttgt
     3601 ttgattatga taacttgatt gcaaaatatt attaacaatt aaagcaatta tgttacagca
     3661 aaatggataa tattgatgtt ttcgcggcga gatcacagtt tgtaaattct tcccgccgga
     3721 gtgaatgcgg ttacctacac tccagattac tgaccactgg aggcagacac tatggcgaac
     3781 agcatcacgg cggatgagat tcgggaacag ttttcgcagg caatgtcagc catgtaccag
     3841 caagaagttc cgcagtacgg cacgctgctg gaactggtag ctgatgtgaa tctggctgtg
     3901 ctggaaaaca atcctcaact gcacgaaaaa atggtaaatg cagacgagct ggcgcgactg
     3961 aatgttgaac gtcatggggc aattcgcgtt gggactgcac aagagcttgc tactcttcgg
     4021 cggatgtttg ccattatggg gatgtacccg gtgagctatt acgatctctc gcaggcggga
     4081 gtgccggtac attccacagc atttcggccc attgatgatg cttctctggc gcgtaatccc
     4141 ttccgcgttt ttacttcatt actccgcctt gagcttatcg agaacgaaat tttgcgccag
     4201 aaagcggagg agattctgcg tcagcgcgat atcttcaccc cacgttgtcg acaactgtta
     4261 gaggaatatg agcagcgggg cggttttaac gaaacacagg cacaggagtt tgtgcaggaa
     4321 gccctggaaa cgtttcgttg gcaccagtca gcaacggtag atgaagaaac ctatcgcgcc
     4381 ttgcacaacg aacatcggtt gattgctgat gtggtctgtt ttcctggatg ccatatcaac
     4441 cacctgacgc cacgtacgct ggatattgac cgggtgcagt cgatgatgcc tgaatgcggg
     4501 attgaaccca aaattctgat cgaagggccg ccgcgccgcg aggtaccgat tttactacgc
     4561 cagaccagct ttaaagcact ggaagagacg gtgttgtttg cagggcagaa acagggtacg
     4621 cataccgcgc gctttggtga aatagagcag cgtggtgtgg cattaacgcc gaaagggcga
     4681 caactgtatg atgatcttct gcgtaacgct ggaaccgggc aggataatct cactcaccaa
     4741 atgcatttac aggaaacctt ccgcactttt cctgacagtg agtttttaat gcgtcagcaa
     4801 gggttggcat ggttccggta ccgtctgacg ccttcaggtg aggcgcatcg tcaggcgatt
     4861 cattccggag acgatccaca gcccttaatt gaacgtggtt gggtagtggc gcaacccatt
     4921 acctatgaag atttcttgcc tgttagcgcg gcggggatct tccagtcaaa tctgggtaat
     4981 gaaacgcagg cacgcagtca cggtaatgcc agtcgcgaag catttgagca ggcgttgggc
     5041 tgcccggttt tggatgagtt ccagctttat caggaagcgg aagaacgcag taaacgtcgc
     5101 tgtggtttgc tttaaattct gaccatccgc ctttgcaaaa atttgcctga ttttacaaac
     5161 gaatcaggct catcccatcg acataaaaaa attgccgatt tatgcatatt ctctcagttc
     5221 aacaattgga ttattaataa atattgtcta gagtgagcgg tcataaataa gcactttctt
     5281 gccgctgaaa acgaccagcg cgggaccatt cacaacacca gaaggactca ctttcaggta
     5341 tggatcgtag acgatttatt aaaggttcaa tggctatggc cgccgtgtgc ggtaccagcg
     5401 gcattgcttc tcttttttct caggcggcat tcgcggcaga ttctgatatt gccgacgggc
     5461 aaacccaacg ttttgacttc tccattctac agtcaatggc gcacgactta gcgcaaacag
     5521 cgtggcgtgg tgcgcctcgt ccgttacctg acacgctggc gacaatgacg ccgcaggctt
     5581 ataacagtat tcaatacgac gccgaaaaat cgctctggca taacgttgag aaccgtcaac
     5641 tggacgctca gttcttccat atgggaatgg gattccgtcg ccgcgttcgt atgttttctg
     5701 ttgatcccgc aacacatctg gcgcgtgaaa ttcactttcg cccggagttg ttcaaataca
     5761 acgatgcggg tgttgatacc aaacaattag aagggcaaag cgatctcggt tttgccggtt
     5821 ttcgcgtgtt taaagccccc gaactggcgc gccgtgatgt cgtatcattc ctcggcgcga
     5881 gttatttccg cgcggttgat gatacatatc aatacggtct atcggctcgc ggcctggcga
     5941 tcgacactta caccgacagt aaagaagagt tccccgactt tactgccttc tggtttgata
     6001 cggtaaaacc gggggcaacc acctttaccg tttatgcgtt gctcgatagc gccagcatta
     6061 ctggtgccta taagttcact atccattgtg agaaaagtca ggtgattatg gatgtggaaa
     6121 atcacctgta tgcgcgcaaa gacattaaac agctgggcat tgcgccgatg accagcatgt
     6181 tcagctgcgg tactaatgaa cgtcggatgt gcgacaccat tcatccgcaa atccatgact
     6241 cagatcgtct gtccatgtgg cggggcaatg gcgagtggat ttgccgtccg ctgaataatc
     6301 cgcaaaaatt gcagttcaat gcttacaccg acaacaaccc gaaagggttt ggtctattgc
     6361 aactggatcg tgatttctct cattatcagg acattatggg ctggtataac aaacgcccaa
     6421 gtctgtgggt ggaaccgcgt aacaagtggg gtaagggcac catcggcctg atggaaatcc
     6481 caacaacggg cgaaacgctg gataacattg tctgcttctg gcagccagaa aaagctgtaa
     6541 aagcgggtga tgagtttgca ttccagtatc gtctgtactg gagtgcgcaa ccgcctgttc
     6601 attgcccatt agcgcgcgtt atggcgacgc gtaccggcat gggtggtttc ccggaaggtt
     6661 gggcgccagg tgaacactat cccgaaaaat gggcgcgtcg ttttgccgtc gatttcgttg
     6721 atggtgatct gaaagctgcc gcaccaaaag gcattgagcc ggtgattacg ctttccagtg
     6781 gggaagcgaa gcaaatcgaa attctctata ttgaacccat tgatggttat cgtattcagt
     6841 ttgactggta tccgacttcg gactccactg atccggtcga tatgcggatg tatctgcgtt
     6901 gtcagggcga cgctatcagt gaaacatggc tctatcagta tttcccgcca gcgccggata
     6961 aacgtcagta tgttgacgac cgcgtgatga gttaattgtt ttttcttcgg caccttcttc
     7021 gggaggtgcc gtctggttaa acacgatccc gctcgcattt ttccctaagt taaatgagta
     7081 atctgatggt gtgtatttca gatacacctt gtcagccact aacagggagt gcgtatgttt
     7141 ccagaatatc gagatttaat atcccgtctg aaaaacgaaa atcctcgctt tatgtccttg
     7201 ttcgataaac acaataaact tgatcatgaa attgccagaa aggaaggttc cgacggtcga
     7261 gggtataatg cggaagtggt ccgcatgaaa aaacaaaagt tacagttaaa agatgagatg
     7321 ctcaaaatcc tgcagcagga gagcgtcaaa gaggtgtaaa ctttcctaag ccgccagcca
     7381 ggcggctttt ttaacaactg catggattga ctggagataa gatgaccgaa acgataaaag
     7441 taagcgaatc acttgaatta catgctgttg cagaaaatca cgtcacacct ctttatcagt
     7501 taatctgtaa aaataaaacc tggttacagc agtcgctaaa ctggccgcag tttgttcaaa
     7561 gtgaagagga cacgcgaaaa acggtgcagg gcaatgtaat gttgcatcaa cgcggatatg
     7621 ccaaaatgtt catgattttc aaagaagatg aacttatcgg cgttatctcg tttaatcgta
     7681 ttgaaccact gaataaaacc gctgaaatcg gctactggct ggacgaatct catcaggggc
     7741 aggggatcat ttctcaggcg ctgcaggcat tgattcatca ttacgcccag tctggtgaac
     7801 ttagacgctt cgtgatcaaa tgtcgggtgg acaatgtgca aagcaaccag gtcgccttgc
     7861 gcaatggttt tatccttgaa ggttgtctga aacaggctga gttcctgaat gatgcctatg
     7921 atgatgtgaa cttatacgcg cgtattatcg attcacaata accctgccag cggcgttcgc
     7981 gtaatgcgct cctcgccgtt aatgactttt ggaccacgga catgaaccgt gtcgccatca
     8041 aaggcttcaa cgacggcgtg gtcggttaac tccacatgtt tttcaattat caccgcacct
     8101 gtaatacggc tctcaccttg tattacaacg tgctcgtcga gcagaatcgg tccgccacgt
     8161 accacggcat ttccgccaat caagacatga tgttttaaca cacaattacc ttccacaatg
     8221 gcatattccg ccacctgcga actgtaatga atcgtcggaa tggcatcttc ttctatgcca
     8281 gctttcacct gcgcgtgacc gtagacttta gcgcaatcgc atagccagac gttgttctct
     8341 tcattacctt caacgctggc aaaatcaaaa acttcggcgc gatgttcaat aaaagcatac
     8401 cggacgacgg catcgccata aatttgtgcc tggtgaacaa tacgcgaggc gctaaccctt
     8461 gcgcgatcat aaatttgtag caggagttga tggtcgggcg ttaagccttg tgcggcgacg
     8521 atcatagaat gttgatcaat taaggcgtga ccaaatattc tacattgacc acaaactaat
     8581 gagtcacgaa tggtgacgct gtcacttata tatgcatcct ggctgatttc actattatcg
     8641 atccagacgt tatccgttgc gtaaacctct ccccaaagaa cgctggtgcc ggtgatacgc
     8701 gtattaccgg aaatcaccgt tccgccgaac gcaatagcat tctgatcgta aatccagcaa
     8761 tttccttcct gtgctaacac cgtttcgcga tcgatccagc cgccagcggt tcctgctatc
     8821 acatcgttaa aatcgctaat ggcgataatc tgccgtaata acacactttt tttagtgcca
     8881 tcttcctgat aactaaaggc tcgctgttct tcacttagac gatatttgcg catagcgttt
     8941 tcccacaggt gacttactat aaccgtagca aattctgcgg ctctggctat gctcgagaaa
     9001 attccataaa atgcatttca aatatacttt ataaattaaa caaaatgagt aagaagatgc
     9061 agagcgataa agtgctcaat ttgccggcag gctactttgg tattgtgttg gggacgatag
     9121 ggatgggatt tgcctggcgc tatgccagcc aggtttggca ggtcagccac tggttagggg
     9181 atgggctggt gattctggcg atgatcatct ggggattatt aactagcgca tttattaccc
     9241 gactcatccg ctttccgcat agcgtgctgg cggaagtgcg ccatccagtg ctgagcagtt
     9301 ttgtgagttt gtttccggca acgacgatgc tggtggcgat tggttttgtt ccgtggtttc
     9361 gcccactggc ggtgtgcctg ttcagttttg gtgtcgtggt gcaactggct tatgccgcat
     9421 ggcaaactgc gggattatgg cgcggatctc accctgaaga agctaccacg cctggactgt
     9481 atctgccgac agttgccaac aactttatca gcgcaatggc ctgtggtgcg ttgggctaca
     9541 ccgacgccgg tctggtgttt ttaggcgcag gcgttttctc atggctaagc cttgaaccgg
     9601 tgatcttgca gcgtctgcgc agttcgggag aattacccac ggcgctgcgg acatcactcg
     9661 gcattcagct cgctcctgcg ctggtggcct gtagtgcctg gctgagcgtc aacggcggcg
     9721 agggtgacac gctggcgaaa atgcttttcg gttatggact gctgcaactg ctgtttatgc
     9781 tacgtctgat gccatggtat ctctcccagc catttaatgc ttcattctgg agtttctcgt
     9841 tcggcgtatc tgcactggca accaccggtt tgcatctggg gagtggcagc gataatggat
     9901 ttttccatac gctggcggtg ccgctgttta tctttaccaa ttttattatt gcaatactgc
     9961 tcatccgtac ttttgcgctt ctgatgcagg gaaaattgtt agtcagaacc gagcgcgccg
    10021 ttttaatgaa agcagaggac aaagaatgat cattcgtgac gaaaactatt ttactgataa
    10081 atatgaatta acccgcacac actctgaagt actggaagcg gtgaaagtgg ttaaaccggg
    10141 taaaacgctg gatctgggct gtggcaatgg tcgtaacagt ctttacctgg cagccaatgg
    10201 ttatgatgtt gacgcatggg ataaaaatgc catgagtatc gccaacgtcg agcgcattaa
    10261 gtccattgaa aatctggata atttacacac ccgggtcgtt gatctgaata acctcacatt
    10321 tgatggacag tacgatttta ttctttcgac tgtggtgctg atgttccttg aggctaaaac
    10381 catcccaggg ctgattgcca atatgcaacg ttgcactaaa cctggcggtt acaacctgat
    10441 tgtggcggcg atggataccg ctgattatcc atgtaccgtc ggcttcccgt ttgccttcaa
    10501 agagggggaa ttacgtcgat attacgaagg ctgggagatg gtgaaataca atgaagacgt
    10561 cggcgagctg caccgcaccg acgccaacgg taatcgtatt aaactgcgtt tcgccacgat
    10621 gctggcacgt aaaaaatgat ccggtaagaa caaagcgcgt gaaaattccc cacgctgaga
    10681 tggtttactg ttcttctttt cggtaagcat attttttatc gaagggatgt gaaattaatc
    10741 acagtagtcg aagtttttta gccgcttaac ttactgaaat ttaactactg atgattgact
    10801 tagccccttt ttcggcattg actatgtcgt ctgaaaaggg gctaaaaaat ttattttcac
    10861 caacactttt tttgccacaa cacgaagcgg cgctttttgc tataacttag aaagtaatat
    10921 aatcatctca ggaaactatt catgcgtacc acatcatttg cgaaagttgc agctttatgc
    10981 ggcttgttgg ctctgtctgg ttgtgcctct aaaatcaccc agccagataa atattctggt
    11041 tttttaaaca attactctga tttaaaagaa acaacctcgg caacaggtaa acctgtttta
    11101 cgttgggtag acccaagttt tgatcaaagc aaatatgaca gcatcgtctg gaacccaatc
    11161 acttattatc cggtaccgaa accttctacc caggtagggc agaaagttct ggataaaatt
    11221 ttgaactata ccaacaccga aatgaaagaa gcgatagcgc agcgtaaacc actggttacc
    11281 accgctgggc cgcatagtct gattttccgt ggggccatta ccggtgtaga taccagcaaa
    11341 gaagggctgc aattctatga agtggttcct gttgcattag tggttgcggg gacgcaaatg
    11401 gctacaggcc accgtaccat ggatactcgc ctctattttg aaggtgagct gattgatgca
    11461 gcgactaata aaccggttat caaagtcgtt cgtcagggcg aaggtaaaga cctgaataac
    11521 gaaagtacgc caatggcttt cgaaaatatt aaacaagtta ttgatgacat ggcgaccgat
    11581 gccaccatgt ttgacgttaa caaaaagtag ttccagacgc gccat
//
LOCUS       DAPOZO010000134        11435 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPOZO010000134 DAPOZO010000000
VERSION     DAPOZO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.134"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(66..617)
                     /gene="papX"
                     /locus_tag="ROI21_004216"
     CDS             complement(66..617)
                     /gene="papX"
                     /locus_tag="ROI21_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PapX"
                     /protein_id="HDX5113069.1"
                     /translation="MRACTQTVCFSNMENNEMNNTDTLEKIIRHQKNKDPAYPFREHL
                     LMQLCIRTNKRMQDNISEFLGVYGINHSVYMVLTTLFAAESHCLSPSEISQKLQFTRT
                     NITRITDFLEKAGYVKRMDSREDRRAKKISLTSEGMFFIQRLTLAQSMYLKEIWGYLT
                     HDEQELFEVINKKLLAHLDDVSS"
     gene            complement(880..1890)
                     /gene="papG-II"
                     /locus_tag="ROI21_004217"
     CDS             complement(880..1890)
                     /gene="papG-II"
                     /locus_tag="ROI21_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151505562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria tip G-adhesin PapG-II"
                     /protein_id="HDX5113070.1"
                     /translation="MKKWFPALLFSLCVSGESSAWNNIVFYSLGDVNSYQGGNVVITQ
                     RPQFITSWRPGIATVTWNQCNGPGFADGSWAYYREYIAWVVFPKKVMTKNGYPLFIEV
                     HNKGSWSEENTGDNDSYFFLKGYKWDERAFDTANLCQKPGEKTRLTEKFDDIIFKVAL
                     PADLPLGDYSVTIPYTSGMQRHFASYLGARFKIPYNVAKTLPRENEMLFLFKNIGGCR
                     PSAQSLEIKHGDLSINSANNHYAAQTLSVSCDVPANIRFMLLRNTTPTYSHGKKFSVG
                     LGHGWDSIVSVNGVDTGETTMRWYKAGTQNLTIGSRLYGESSKIQPGVLSGSATLLMI
                     LP"
     gene            complement(1934..2434)
                     /gene="papF"
                     /locus_tag="ROI21_004218"
     CDS             complement(1934..2434)
                     /gene="papF"
                     /locus_tag="ROI21_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042092232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial tip protein PapF"
                     /protein_id="HDX5113071.1"
                     /translation="MARLSLFISLLLTSVAVLADVQINIRGNVYIPPCTINNGQNIVV
                     DFGNINPEHVDNSRGEITKTISISCTYKSGSPWIKVTGNAMAGQTNVLATNIANFGIA
                     LYQGKGMSTPLTLGNGSGNGYRVTAGLDTARSTFTFTSVPFRNGSRTLNGGDFRTTAS
                     MSMIYN"
     gene            complement(2509..3036)
                     /locus_tag="ROI21_004219"
     CDS             complement(2509..3036)
                     /locus_tag="ROI21_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX5113072.1"
                     /translation="MKKIRGLCLPVILGAVLMSQHAHAADNLTFKGKLIIPACTVTKA
                     EVDWGNVEIQTLSQNGNHEKEFTVNMQCPYNLGTMKVTITATNTQNNNSILVPNTSTT
                     TGNGLLIYLYNRNQNGFIGSPIVFNSPFTPGYIKEVGATRNISLYAKLGYKGNMQNLQ
                     AGAFAATATLVASYS"
     gene            complement(3063..3599)
                     /locus_tag="ROI21_004220"
     CDS             complement(3063..3599)
                     /locus_tag="ROI21_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX5113073.1"
                     /translation="MIKSTGALLLFAALSAGQAIASDVAFRGNLLDRPCHVSGDSLNK
                     HVVFKTRASRDFWYPPGRSPTESFVIRLENCHATAVGKIVTLTFKGTEEAALPGHLKV
                     TGVNSGRLGIALLDTDGSSLLKPGASHNKGQGEKVTGNSLELPFGAYVVATPEALRTK
                     SVVPGDYEATATFELTYR"
     gene            complement(3609..4190)
                     /locus_tag="ROI21_004221"
     CDS             complement(3609..4190)
                     /locus_tag="ROI21_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein papJ"
                     /protein_id="HDX5113074.1"
                     /translation="MVVNKTTAVLYLIALSLSGFIHTFLRAEERGIYDDVFTADELHH
                     YRINERGGRTGSLAVSGALLSSPCTLVSNEVPLSLRPENHSASAGAPLMLRLAGCGDG
                     GALQPGKRGVAMTVSGSLVTGPGTGSALLPDRKLSGCDHLVIHDGDTFLLCRPDRRQE
                     EMLAAWRKRATQEGEYSDARSNPAMLRLSIKYE"
     gene            complement(4227..4961)
                     /locus_tag="ROI21_004222"
     CDS             complement(4227..4961)
                     /locus_tag="ROI21_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDX5113075.1"
                     /translation="MWKLSMIRKKILMAAIPLFVISGADAAVSLDRTRAVFDGSEKSM
                     TLDISNDNKQLPYLAQAWIENENQEKIIAGPVIATPPVQRLEPGAKSMVRLSTTPDIS
                     KLPQDRESLFYFNLREIPPRSEKANVLQIALQTKIKLFYRPAAIKTRPNEVWQDQLIL
                     NKVSDGYRIENPTPYYVTVIGLGGSEKQAEEGEFETVMLSPRSEQTVKSANYNTPYLS
                     YINDYGGRPVLSFICNGSRCSVKKEK"
     gene            complement(5032..7542)
                     /gene="papC"
                     /locus_tag="ROI21_004223"
     CDS             complement(5032..7542)
                     /gene="papC"
                     /locus_tag="ROI21_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial usher protein PapC"
                     /protein_id="HDX5113076.1"
                     /translation="MKDRIPFAVNNITCVILLSLFCNAASAVEFNTDVLDAADKKNID
                     FTRFSEAGYVLPGQYLLDVIVNGQSISPASLQISFVEPQSSGDKAEKKLPQACLTSDM
                     VRLMGLTAESLDKVVYWHDGQCADFHGLPGVDIRPDTGAGVLRINMPQAWLEYSDATW
                     LPPSRWDDGIPGLMLDYNLNGTVSRNYQGGDSHQFSYNGTVGGNLGPWRLRADYQGSQ
                     EQSRYNGEKTTNRNFTWSRFYLFRAIPRWRANLTLGENNINSDIFRSWSYTGASLESD
                     DRMLPPRLRGYAPQITGIAETNARVVVSQQGRVLYDSMVPAGPFSIQDLDSSVRGRLD
                     VEVIEQNGRKKTFQVDTASVPYLTRPGQVRYKLVSGRSRGYGHETEGPVFATGEASWG
                     LSNQWSLYGGAVLAGDYNALAAGAGWDLGVPGTLSADITQSVARIEGERTFQGKSWRL
                     SYSKRFDNADADITFAGYRFSERNYMTMEQYLNARYRNDYSSREKEMYTVTLNKNVAD
                     WNTSFNLQYSRQTYWDIRKTDYYTVSVNRYFNVFGLQGVAVGLSASRSKYLGRDNDSA
                     YLRISVPLGTGTASYSGSMSNDRYVNMAGYTDTFNDGLDSYSLNAGLNSGGGLTSQRQ
                     INAYYSHRSPLANLSANIASLQKGYTSFGVSASGGATITGKGAALHAGGMSGGTRLLV
                     DTDGVGGVPVDGGQVVTNRWGTGVVTDISSYYRNTTSVDLKRLPDDVEATRSVVESAL
                     TEGAIGYRKFSVLKGKRLFAILRLADGSQPPFGASVTSEKGRELGMVADEGLAWLSGV
                     TPGETLSVNWDGKIQCQVNVPETAISDQQLLLPCTPQK"
     gene            complement(7601..8188)
                     /gene="papH"
                     /locus_tag="ROI21_004224"
     CDS             complement(7601..8188)
                     /gene="papH"
                     /locus_tag="ROI21_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial minor subunit PapH"
                     /protein_id="HDX5113077.1"
                     /translation="MRLRFSVPLFFFCCVFVHGVFAGPFPPPGMSLPEYWGEEHVWWD
                     GRAAFHGEVVRPACTLAMEDAWQIIDMGETPVRDLQNGFSGPERKFSLRLRNCEFNSQ
                     GGNLFSDSRIRVTFDGVRGETPDKFNLSGQAKGINLQIADARGNIARAGKVMPAIPLT
                     GNEEALDYTLRIVRNGKKLEAGNYFAVLGFRVDYE"
     gene            complement(8258..8824)
                     /locus_tag="ROI21_004225"
     CDS             complement(8258..8824)
                     /locus_tag="ROI21_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDX5113078.1"
                     /translation="MIKSVIAGAVAMAVVSFGAYAAPTIPQGQGKVTFNGTVVDAPCG
                     IDAQSADQSIDFGQVSKLFLENDGESQPKSFDIKLINCDITNFKKAAGGGGAKTGTVS
                     LTFSGVPSGPQSDMLQTVGATNTAIVVTDPHGKRVKFDGATATGVSYLVDGDNTIHFT
                     AAVRKDGSGNPVTEGAFSAVANFNLTYQ"
     gene            complement(9031..9345)
                     /locus_tag="ROI21_004226"
     CDS             complement(9031..9345)
                     /locus_tag="ROI21_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon regulatory protein
                     PapB"
                     /protein_id="HDX5113079.1"
                     /translation="MAHHEVISRSGNAFLLNIRESVLLPGSMSEMHFFLLIGISSIHS
                     DRVILAMKDYLVGGHSRKEVCEKYQMNNGYFSTTLGRLIRLNALAARLAPYYTDESSA
                     FD"
     gene            9750..9983
                     /gene="papI"
                     /locus_tag="ROI21_004227"
     CDS             9750..9983
                     /gene="papI"
                     /locus_tag="ROI21_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major pilus subunit operon transcriptional
                     regulator PapI"
                     /protein_id="HDX5113080.1"
                     /translation="MSEYMKNEILEFLNRHDGGKTAEIAEALAVTDYQARYYLLLLEK
                     AGMVQRSPLRRGMATYWFLKGEKQAGQSCSSTT"
     gene            complement(10547..11435)
                     /locus_tag="ROI21_004228"
                     /pseudo
     CDS             complement(10547..11435)
                     /locus_tag="ROI21_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(11362..>11435)
                     /locus_tag="ROI21_004228"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         3055 a         2975 c         2361 g         3044 t
ORIGIN      
        1 acacatctgc aagaatcatt attcttttgc ctgaagctat ccggcatact caggcatttc
       61 acgctttatg agctgacatc atcaagatgc gccagtaatt ttttattaat gacttcaaac
      121 agttcctgtt catcatgggt cagataaccc cagatttctt tcagatacat gctttgtgca
      181 agagtgagcc tctgaataaa aaacatacct tcagatgtca gactgatttt tttagcacgg
      241 cgatcctccc tgctatccat cctttttaca tatccggctt tttctaaaaa atctgtaatg
      301 cgggtaatat tagttctggt aaactgaagt ttctggctta tctctgaagg tgacagacaa
      361 tgactctccg ctgcgaataa tgtggtgaga accatatata ctgagtgatt tattccatac
      421 acccccagaa attcagatat attatcctgc attcttttat ttgtgcgaat acagagttgc
      481 atcaacaaat gctcccgaaa gggatatgcg gggtctttgt ttttttggtg tctgattatt
      541 ttttctaatg tgtctgtgtt attcatttca ttattctcca tattactgaa acacactgtc
      601 tgtgtacaag cgcgcatcag tcatccggta aacatcgact ttccactaca atttattcct
      661 gcctgatttc tgtataactg agatattcct acacagcaga cagataaatt ccctcagata
      721 aaagaacata aatgctggct gctgacaagt ctgtgaaaaa ggattatcca tactgctcag
      781 taatcgcgga tcattcacca gccctccgcc cgtctgaaag gtgacttccc ggggcatccc
      841 agatatccac aacactctat ccggctccgg ataaaccatt tatggcaata tcatgagcag
      901 cgttgctgaa ccagatagta ctcctggttg tatctttgaa gattcaccat agaggcgact
      961 gccgatggtc aggttttgtg tacctgcttt gtaccatctc atcgttgtct ctcctgtgtc
     1021 caccccgtta accgaaacaa tggagtccca gccatgcccc agaccaaccg aaaatttctt
     1081 accatggctg tatgtcggag ttgtatttct taacagcata aaacgaatat ttgcaggcac
     1141 atcgcaagac acagaaagag tctgagccgc ataatgatta ttagcgctat taatagacag
     1201 atcaccatgc tttatttcca gagactgtgc agaaggacgg catccgccga tattcttaaa
     1261 taagaataac atttcattct ctcttgggag ggttttggcc acattgtatg ggattttaaa
     1321 acgggccccc aagtaactcg cgaaatgacg ctgcatgccg gaagtgtatg gaattgtaac
     1381 agaataatcc cctaaaggaa gatctgcagg tagggcgact ttaaaaataa tatcgtcaaa
     1441 tttctcagtc agacgggttt tttctcctgg tttctgacac aaattagctg tatcaaaggc
     1501 ccgctcatcc cacttatacc ccttgagaaa aaaatagctg tcattgtcac cagtattctc
     1561 ctcactccag ctacctttat tatgaacctc aataaataag ggatatccat ttttggtcat
     1621 aacctttttg gggaatacta cccacgcaat atactccctg tagtaagccc aggaaccatc
     1681 agcaaaccca ggaccattac actgattcca ggttaccgta gcaatgcccg ggcgccacga
     1741 agttataaat tgtggccttt gagtaatcac cacattccct ccctgataag agttaacgtc
     1801 tccaagggag taaaagacaa tattattcca tgcagaggac tcaccagaca cacacaagga
     1861 aaataacaaa gctgggaacc attttttcat cagaaatcac ttccgtaatt acagttattc
     1921 atttaggtat gactcagtta taaatcatgc tcatactggc cgtggtccgg aaatccccgc
     1981 cattcagtgt cctgctgcca ttacgaaagg gcactgaagt aaaggtgaac gttgaacgtg
     2041 ctgtgtccag acctgctgtc actctgtaac catttcctga accattacct aatgtaagag
     2101 gtgttgacat tccttttccc tgatacaacg ctataccaaa attggctata tttgttgcca
     2161 gtacattagt ctgcccagcc attgcattac ctgtgacctt tatccagggt gagccactct
     2221 tatacgtaca ggatatgctt atggtttttg tgatttcacc acgtgagttg tccacgtgct
     2281 caggattaat attcccaaaa tcaacaacaa tattctgccc gttattaatg gtgcatgggg
     2341 ggatataaac atttcccctg atgttaatct gcacatcagc cagtacagcg accgatgtca
     2401 gaagcaacga tataaataat gataaacgag ccattccccc tccggagagc ggtacagaaa
     2461 atattttatt ttatgagata taaaatcggc gtattttaac tgatagcatt acgaatatga
     2521 tgcaaccagc gttgctgttg cagcgaatgc ccctgcctgc aagttctgca tatttccttt
     2581 atatccaagt tttgcataga gagaaatatt tctggtcgcc cccacctctt taatatagcc
     2641 aggggtaaat ggcgaattaa aaactatagg tgaaccaata aaaccatttt gatttctgtt
     2701 gtaaagatag ataagcaatc cattacctgt cgtagttgat gtattaggca ccagtataga
     2761 attgttattc tgagtattcg tggctgttat cgtaaccttc attgttccca gattataagg
     2821 acactgcata ttcacagtaa actctttttc gtgatttcca ttttgactca gggtctgaat
     2881 ctctacattt ccccagtcaa cctctgcctt tgttacagta caggctggaa taatcagttt
     2941 tcctttaaag gtcagattat cagctgcatg tgcatgctga gacattaaca ctgcccccag
     3001 tattacggga agacacaaac ctcttatctt tttcatctga aatatcctgt acaaaatttt
     3061 ttctaacgat atgtcaattc aaacgtggct gttgcttcat aatcaccggg taccacactc
     3121 ttcgtccgca gggcttccgg cgttgccaca acatacgcgc cgaaaggaag ctcaagactg
     3181 tttccggtaa ccttttcccc ctggcctttg ttatgggaag cgccgggttt cagcagactg
     3241 ctgccatcgg tgtccagcag tgcaatgcct aaccggccgg aattcactcc ggttaccttc
     3301 agatggcccg ggagggccgc ctcttccgtt cccttaaagg tcagggtcac aattttgcca
     3361 actgctgttg catggcagtt ttccagcctg atgacaaacg actctgtcgg cgaacgtccg
     3421 ggcggatacc agaaatccct ggacgcccgg gttttgaaga cgacatgttt attcagactg
     3481 tcaccggaca catggcaggg tctgtcaagc agattacccc tgaatgccac atctgaggct
     3541 attgcctgtc cggcagacag tgcggcaaac agtaaaagag cgcctgtgct ttttatcatc
     3601 acattccctt actcatattt tatgctcaga cgcagcatgg ccggattgct cctggcatca
     3661 gaatactcac cctcctgtgt cgcccttttc ctccaggcgg ccagcatctc ctcctgccgc
     3721 cggtcaggcc ggcacagtaa aaaggtatca ccatcatgta taacaagatg gtcacagccg
     3781 gatagcttac ggtcaggaag taaagcactt ccggttccgg gaccggttac cagtgagccg
     3841 gagactgtca tcgcaacgcc ccgttttccg ggctgaagtg caccaccgtc cccacatcct
     3901 gccagcctca gcatcagagg tgctccggct gacgcagagt gattttccgg ccggaggctt
     3961 aacggcacct cattactcac cagcgtgcag ggtgaggaca gcagtgcacc actgactgcc
     4021 aggcttccgg tgcgtccccc ccgttcattt atccggtaat gatgcaactc atctgcagta
     4081 aagacgtcat cgtatatacc ccgctcttca gcccgcagga aagtatggat gaaaccactc
     4141 agcgacagtg caataagata cagtactgct gttgttttat tcacaaccat aatatcccac
     4201 ccgcatttaa ccgttattgc ggtacattat ttctcttttt tcacagagca acggctgcca
     4261 ttacagataa acgacagtac cgggcgacca ccatagtcgt taatataaga cagataaggg
     4321 gtattataat ttgccgattt tactgtctgc tctgaacggg gagacagcat cacggtttca
     4381 aactcacctt cctctgcctg cttttcactt cctcccagac caataacagt gacataatag
     4441 ggggtcgggt tttcaatacg atacccatcg ctgactttgt tcagaattaa ctggtcctgc
     4501 catacttcat ttggtctggt tttaattgct gccgggcgat aaaaaagctt tattttggtc
     4561 tgtaaggcta tctgcagtac attggccttt tcactcctcg gcggtatttc ccggagatta
     4621 aaataaaaca gtgattccct gtcctgagga agtttactga tatccggtgt ggtactcagc
     4681 ctgaccatgc ttttcgcacc cggctcaagg cgctgaaccg gaggggtggc aataaccggc
     4741 cctgcaataa ttttttcctg attttcattt tctatccatg cctgagcaag atagggcagt
     4801 tgtttgttat cattggagat atcaagcgtc attgacttct cactcccgtc aaacaccgcg
     4861 cgggttctgt ccagcgaaac agcagcgtct gccccggata taacaaacag ggggatggca
     4921 gccatcagaa tcttttttcg aatcatactt aatttccaca ttctgtaatt tcacctggtc
     4981 cggaaaatgg cataaccgca ttcatcagcc gttaatattc cggactttca tttatttctg
     5041 aggcgtacag ggaagcaata actgctggtc agatattgct gtctccggta catttacctg
     5101 acactgtatt tttccatccc agtttaccga cagggtttcc cccggcgtca cgccactcag
     5161 ccaggcaagg ccttcgtcgg ccaccatgcc cagctcccgg cctttttcac tggttacact
     5221 ggcaccaaac gggggctgag agccatcagc aagacgcagt attgcaaaca gacgtttccc
     5281 tttaagcacg ctgaatttcc ggtaaccaat ggcaccttct gtcagcgccg attccacaac
     5341 agaacgggtt gcttccacat catccggtaa gcgcttcagg tcaacagagg ttgtattccg
     5401 gtaataactg ctgatgtcag tcaccacgcc cgttccccag cgatttgtca ccacctgccc
     5461 gccatcaacc ggtacacctc ccacaccatc cgtgtcaaca agaagacgtg ttccaccgga
     5521 cattccccct gcatgtaacg ccgcaccttt tccggtaatt gttgccccac cggaagcact
     5581 gacgccgaaa gacgtatatc ctttctgcag ggatgcaata ttcgcggaca aatttgccag
     5641 cggactacga tgactgtaat aggcattaat ctgacgttgc gatgtcagtc caccgccact
     5701 gttaaggccg gcgttcaggc tgtagctgtc cagaccgtca ttgaacgtgt cagtgtagcc
     5761 ggccatattc acataacggt cattactcat actgccactg tagctcgcag tccccgtccc
     5821 cagcggcacg gatatacgca ggtaagcaga atcgttatca cgccccagat atttagacct
     5881 tgaggctgac aatccaaccg ccacaccctg cagtccgaaa acattaaagt agcggttgac
     5941 gctcaccgta taatagtccg ttttccgtat gtcccagtat gtctgacggc tgtactgcag
     6001 gttaaaagag gtgttccagt ccgccacgtt tttattcagc gtaacggtat acatctcttt
     6061 ttcccgactg ctgtaatcat tacggtagcg ggcgttcagg tactgctcca tggtcatata
     6121 gtttcgctct gagaaacgat acccggcgaa cgtaatgtcg gcatccgcat tatcaaaccg
     6181 tttggagtag ctaagacgcc aggattttcc ctgaaacgtt ctctctccct caatacgggc
     6241 tactgactgc gtgatatcag cggaaagggt ccccggcaca cccaggtccc agccggcacc
     6301 ggctgccagt gcattataat caccggcaag cacagccccg ccatacagcg accactggtt
     6361 actgagcccc caggacgcct ctccggtcgc aaatacaggc ccttcggtct catgcccgta
     6421 tccgcgggaa cgaccggaga caagtttata ccggacctgt cccggacgcg tcagataagg
     6481 aaccgaggcc gtatcgacct gaaaggtttt cttccgtccg ttctgttcaa taacctcaac
     6541 atcaagacgt ccgcgaactg aactgtccag gtcctgaata ctgaatggcc ctgcggggac
     6601 catcgagtcg tacagcaccc gtccctgctg cgacaccaca acacgggcat tagtctccgc
     6661 aatcccggta atctgcggtg catagcctcg cagtctgggc ggcagcatcc ggtcatcgct
     6721 ttccaggctg gctcccgtat aactccatga ccggaatata tctgagttga tattattctc
     6781 gcccagcgtc aggtttgccc gccaccgggg aatggcacgg aacagataaa agcgactcca
     6841 tgtgaaattt ctgtttgtcg ttttttcccc gttgtagcgg ctctgctcct ggcttccctg
     6901 atagtcagcc cgcaggcgcc agggccccag attccccccc acagtcccgt tataactgaa
     6961 ctgatgagag tctcctccct gataattacg ggaaaccgtc ccgttgaggt tataatccag
     7021 catcagtccg ggaatgccgt cgtcccagcg tgagggaggc agccaggtgg catcagaata
     7081 ctcaagccag gcctgcggca tattgatgcg taatacgccc gctccggtat caggacgaat
     7141 atccactccc ggcaacccat gaaaatccgc acactgacca tcatgccagt aaacaacttt
     7201 atccagagat tctgctgtta accccatcag tctgaccata tctgatgtca ggcaggcctg
     7261 tggcaatttt ttttctgcct tatctcctga cgactgaggt tcaacaaatg aaatctgtaa
     7321 cgatgcggga gaaatacttt gcccgttaac aatcacatcc agaagatatt gccccggcag
     7381 aacataaccg gcttctgaaa aacgggtgaa gtcaatattt ttcttgtccg ccgcgtcaag
     7441 tacatctgta ttaaactcaa cggcactggc tgcgttacaa aacagagaca acaatatcac
     7501 acaggtaata ttgttgactg caaaaggtat tctgtctttc attccacgca tcaccagatt
     7561 cacaaaaaag ataaataacc ggacatctca ccggagtgac tcactcataa tcgacccgga
     7621 atcccagcac ggcaaaataa tttccggctt caagtttttt tccgtttcgc acaattctga
     7681 gggtgtaatc cagcgcttct tcattacccg tcaatggtat tgcaggcatt actttccctg
     7741 cccgggcaat atttcccctg gcatcagcta tctgcagatt aatgcctttt gcctgaccgg
     7801 ataaattaaa cttatccggc gtttcacccc ggacgccatc gaaagtcacc cttatccggg
     7861 aatcagagaa aaggttccca ccctgactgt taaattcaca gttcctgagc cggaggctga
     7921 attttctttc aggtccggaa aaaccattct gtaaatcccg aaccggggtt tcccccatat
     7981 cgataatctg ccaggcgtct tccatcgcca gagtacaggc aggtctgaca acctcaccat
     8041 gaaaagcagc cctgccgtcc caccatacgt gttcttctcc ccagtattca ggaagggaca
     8101 tgccgggcgg aggaaacgga ccggcaaaaa caccatgaac aaacacacag caaaaaaaga
     8161 aaagtggaac agagaatcgc agtctcatga taaataccct gagaaataaa cagcggaata
     8221 tttccgctgt ttaccggacc agattatcgt attactgtta ctgataagtc aggttgaaat
     8281 tcgcaactgc tgagaaagca ccttctgtta cagggttgcc actaccatct tttctgacgg
     8341 cagcagtaaa atgaattgtg ttatcaccat caactaaata ggaaacacct gttgctgtcg
     8401 caccatcaaa ttttacgcgt tttccatgtg gatcggtgac aacaatagct gtatttgttg
     8461 caccgacggt ctgtaacatg tcactctgcg gaccacttgg gacacccgaa aaagtcagag
     8521 atactgtgcc agtcttcgcc ccaccaccgc cagcagcttt tttaaagttt gtaatatcac
     8581 aatttataag tttaatatca aaagatttgg gttgactttc cccatcattc tccagaaata
     8641 attttgatac ttgtccaaaa tcaatagatt gatcagcaga ctgagcatca ataccacatg
     8701 gtgcatctac tacagttccg ttaaaagtta ctttaccctg cccctgagga atagttggag
     8761 cagcatatgc accaaaagac accactgcca tagctaccgc accggcaata accgacttaa
     8821 tcataaataa caacctcttt ttcattactg aacacaccag agatatctgg caatttaaaa
     8881 tgacccgatt acctttaata accagatcaa tacaaataat attctcgtat ttcagggcag
     8941 gttatttatc gatagaatac tgatataacc tgtctgcttt ctgataaggg cttctttttt
     9001 tatcttccag aaactccgga atgccataat ttagtcaaat gccgaggact catctgtata
     9061 ataaggtgca agccttgctg caagagcatt cagccgtata agtctcccca gtgttgtact
     9121 gaaataccca ttattcatct ggtatttctc gcagacctcc ttacgggagt gcccacctac
     9181 cagatagtcc ttcatagcca gaatgaccct gtcactgtga atagaagaaa tacctatcag
     9241 taaaaaaaaa tgcatttcag acatagaccc gggcaacagt acgctctcgc gtatattcag
     9301 caaaaacgca tttcctgacc gactgatgac ttcatgatgc gccatgtttc ccccttctgt
     9361 cgggcccctg gatatatgct tccggcccgg aaatactctt cacgcaataa gttaaattta
     9421 gttttttatg ttgtaaatat tgatttaaac gatcttttaa cccacaaaac aaaattaaac
     9481 tagaacaaaa cactaatagc aagagggtac tcagataaaa acatcatggc gaattgaatt
     9541 tacagcataa aagatcgtct aaatgaattt ttacggactt tcttttcgca gaaaaataaa
     9601 acgcaaaatg tgatacacat caaataaatc caataaaaag caaattcact aaattgaaca
     9661 aataaagcaa acatagagaa taaaaatacc tttccgttga taacagagag catgattttt
     9721 attattcgaa gaaactttgt tacagtgaga tgagtgaata tatgaagaat gaaatactgg
     9781 aatttctgaa caggcatgat ggagggaaaa cggcagaaat tgcagaggcg ctggcggtaa
     9841 cggattacca ggcccgctat tacctgttat tactggaaaa agcaggtatg gttcagcgct
     9901 caccattaag acggggcatg gcaacatact ggtttctgaa aggagagaag caggcggggc
     9961 agagctgttc ttccacaact taataactca atctgtgggc atgcttcact gaaacagata
    10021 aaagttacat cagagtacag attcaatttt gcagcattca tcacaaaata gaaatttagt
    10081 attttatggc agaatacttc acatgataat aaaaacctgt ctggatttac tgcatggcca
    10141 gacattacaa aatacaaacg ccataaactt cattcagcct tcttgctgtc cggtaacgga
    10201 ggctgctttt tcgttacacc tttccccggc agcatcagta gcagtaattt atagattcta
    10261 cgaactgaga ctgtctgtcc ggggagaacg tacacagccc gacacaatgt accagtcaat
    10321 aatatccggt ttctgattcc ctgcctgaca gaccactctc tggcaacttt gtattcagtt
    10381 catctactac tggagacaga tttataaaat ccaactccac gatctttgac tgtccgcatc
    10441 ccctactctc tgtgctgcgg tcatcggtta attccagcgt ccgtccgtta cgatcagata
    10501 ttactgaacc gccccgggtt tcctggagag tgttttatct gtgaactcag gctgccagat
    10561 cattgtttcc gatggaagca taataagctt tttctgcttc tgccggagga gtatggccca
    10621 gccttcccag caatcgtcga ttgttatacc agtccaccca cgtgagtgtg gccagttcca
    10681 cttctgcacg gtttttccag atcttacggt gtattacctc cgctttgtaa agaccattga
    10741 tgctctccgc catcgcgttg tcatacgagt cgcctgtact ccctgttgat gccagcagtt
    10801 ttgcttcttt taatcgctcc atataggcca gtgacacata ctgagagcct ttatcgctgt
    10861 gatggatggt gccggacgga cggcgggccc acaacgcctg ctccagcgca tccagcatga
    10921 atgtcgtttc catagacgat gagacccacc accccacgat gtatccggca aacacatcaa
    10981 tgatgaacgc cacatagacg aagccctgcc atgtgctgac gtaattaaaa tcagccaccc
    11041 acagctggtc aggacgttct gccacgaact gacggtttac gcggtcacct gcagcaacgg
    11101 ttttccggtt gacgtagtgc ggaccttttt accccggaga acaccggcaa gtcccataac
    11161 cgccatgagg cgcgccactg tacatctggc caccctgatt ccttcccgta acaactgacg
    11221 ccagacttta cgcacaccgt acacctgatg attttcatcg tatacgcgct gtatctcttt
    11281 cttcagccag tcatcgcgct gcgcacgggc actgcgttta tcaggatgat gtctctgctg
    11341 ctgacaatgg taatacgttg acggggcaat atgcagttca ctgcataccg gtccgacccc
    11401 gtactgctca cgcagcttat ccagcagtgg catta
//
LOCUS       DAPOZO010000135        11184 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPOZO010000135 DAPOZO010000000
VERSION     DAPOZO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.135"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..1182
                     /locus_tag="ROI21_004229"
     CDS             <1..1182
                     /locus_tag="ROI21_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDX5113081.1"
                     /translation="QDNTGAVPADEADREQPEETAETVSPVQRLAQLTAELDAQHNDR
                     LLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLAIASLHK
                     HLLTARADLRLPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQEK
                     HTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQSFDKGE
                     MADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEELYPVLADFFKPALLARME
                     VVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVTRAENGARM
                     LESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDDAFTADVEDAQNDESVTKDETV
                     L"
     gene            1179..1922
                     /gene="tagO"
                     /locus_tag="ROI21_004230"
     CDS             1179..1922
                     /gene="tagO"
                     /locus_tag="ROI21_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HDX5113082.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAETSSMAGVAVAVATTT
                     PPDATATSQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRVACHWAGE"
     gene            1927..3339
                     /gene="tssA"
                     /locus_tag="ROI21_004231"
     CDS             1927..3339
                     /gene="tssA"
                     /locus_tag="ROI21_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HDX5113083.1"
                     /translation="MAMDLRDPNVWITHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELALATDDLFALYQRAFNRAAPAPAPTPAASDSSRQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            3448..6882
                     /gene="tssM"
                     /locus_tag="ROI21_004232"
     CDS             3448..6882
                     /gene="tssM"
                     /locus_tag="ROI21_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HDX5113084.1"
                     /translation="MLILAWFFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLAEMGLYQGRR
                     IGPYVERTYLQLLEQRYLPSLFNGLVKEMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGAFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLTVQKDKESTMQAVYLQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWAGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPHSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLVGARGGAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            6893..8245
                     /locus_tag="ROI21_004233"
     CDS             6893..8245
                     /locus_tag="ROI21_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HDX5113085.1"
                     /translation="MNSNGLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAEPDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQPKSNVENAVQTTVRTLIYAPETTASSTPEAVVPPLPDLPEMK
                     VEVRSLSENPPQASVMKQGSTVRGFITGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLGVSPVQPLETGMQMMRVADSRWPESLQQQQASTH
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMRREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNIDALEKQINERLEGVLIRWLLLKNNVMPEAATGTTAEK
                     "
     gene            8269..8751
                     /locus_tag="ROI21_004234"
     CDS             8269..8751
                     /locus_tag="ROI21_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDX5113086.1"
                     /translation="MANMSYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMVTCEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            8795..9709
                     /locus_tag="ROI21_004235"
     CDS             8795..9709
                     /locus_tag="ROI21_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113087.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRRQNTHHVILLTLDVAKKILSSITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDGNGKIIFNFNTLKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACLSFGLPVILVGGAILLTGIVCTVVLNEIDAQYHLSEKLKDAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            9719..10186
                     /locus_tag="ROI21_004236"
     CDS             9719..10186
                     /locus_tag="ROI21_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113088.1"
                     /translation="MRLHVKLKEFFSMFFIAILFFPAFNASLFFTGIKPLYSIIKGST
                     EIFYDWRMLILCFGFISFSLLNIHVILLTIIKSFLIKKTKVINFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNSIKCNNEHISHKED"
     gene            complement(10335..11120)
                     /locus_tag="ROI21_004237"
     CDS             complement(10335..11120)
                     /locus_tag="ROI21_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113089.1"
                     /translation="MNSKKVCCIYVLFSLLAGCVSESLIDEKKKKAQTTQSNFNKNAP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDTALKSTVWSDYKNEKLTNTISLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPFPGKNEASTTDMTEQQIIQLKQKTYKAMVKDLVNRYQ"
BASE COUNT         2775 a         2895 c         2946 g         2568 t
ORIGIN      
        1 caggataata ccggtgccgt accggctgat gaggccgaca gagaacagcc ggaagagacc
       61 gctgaaacag tttccccggt acagcggctg gcccagctca ctgccgaact ggacgcccag
      121 cataacgacc ggttgctggt ctccccgcac gtcgataaaa aacagattgc ggcggtgatt
      181 gccgaatgga ccggcgtgcc gctcaaccgc ctgtcgcaga atgaaatgtc ggtcatcacc
      241 gacctgccga aatggctggg cgacaccatc aaaggccaag acctggcgat tgccagcctg
      301 cataagcatc tactgaccgc acgcgccgac ctgcgtcttc cgggacgccc gctcggtgcg
      361 ttcctgctgg ctggccccag cggtgtgggt aaaaccgaaa ccgtcctgca actggcagag
      421 ctgctctacg gcggtcgcca gtacctgacc accatcaata tgtccgaatt ccaggagaaa
      481 cacaccgtct cgcggctgat tggttcgcct ccgggctacg ttggctacgg tgaaggcggc
      541 gtactgaccg aagcgattcg ccagaaaccc tactcggtag tactgctcga tgaagtggaa
      601 aaagcgcacc cggatgtgct caacctgttc taccagtcgt tcgataaggg cgaaatggca
      661 gacggtgaag gccgcctgat tgactgcaaa aatatcgtct tcttcctgac gtccaacctc
      721 ggctaccagg taatagtcga gcacgccgat gacccggaaa ccatgcagga agaactgtat
      781 ccggtgctgg ccgacttctt caaacctgcc ctgctggcgc gtatggaagt ggtgccgtat
      841 ctgccgctgt cgaaagagac gctcgccacc attatcgccg gaaaactggc ccgtctggat
      901 aacgtgctgc gcagtcgctt tggtgcagaa gtggtcattg aaccggaagt gacggacgaa
      961 atcatgagcc gcgtcacccg cgcggaaaac ggcgcgagga tgctggaatc ggtcatcgat
     1021 ggcgacatgc taccgccgct ctcgctgctg ctgttgcaga aaatggcggc taacacggcg
     1081 attgcccgga ttcggttgtc ggcggtggac gacgcattta cggcagacgt ggaagatgct
     1141 cagaacgacg agtccgtcac aaaggatgaa acggttttat gagcaggttg cgcgttttcc
     1201 ggcagacagg atggtttatc gcgggcctga tgacgggctt acccgccact gccgcgcccg
     1261 cagaaacatc gtcaatggcc ggtgtggcgg tcgccgtcgc caccacgacc ccgccggatg
     1321 cgacagccac gtcacaggcc atgcagtcct gtcggcggga gtccgcggcg ctggagcggc
     1381 ttgactgcta tgaccgcctg ctggcaccgc tgtccccgtc agggtttgac ggggcgctgg
     1441 tcaaagccgg ttttgtgggc gaggcctgga cccgcgccac agagcaggaa aaacgccgcg
     1501 agggcaacac cacagaactg ctggtgacgc aggtgccggg ggagcgcccg acggtggtga
     1561 taaccacccc ggctatcggc catgttccgc cgcgcccggt gctgatgttc agttgcgtgg
     1621 acaatatcac ccggatgcag gtggcgctga tgcaccctct ggatgtccat gatatcgccg
     1681 tcactctgaa tgccgacagc cgggcattgc gtagccactg gtttgtgcgc gagaacggca
     1741 ccctgctgga gtccagccgg gggctgtccg ggattgatga aattaagcaa ctgttcggcg
     1801 caaaaacgct gacagtggat accggcgcag ataacgccgc aggaaagctg acctttaaca
     1861 ttgacgggct ggcaagggcg attgcccccc tgcgcgttgc ctgccactgg gcgggagaat
     1921 aaaaatatgg caatggactt acgcgatccg aatgtctgga taacgcacct gctggaaaac
     1981 ctgccggaag aaaaactggc atcggcgctg aaagatgaca acccggactg ggagtatatc
     2041 gacggcgaaa tcgtcaaact gggatctctg gcccacagtc aactggatat cccggaactc
     2101 cagcgccggg ggctggtcat tctggcttcg gagagtaagg actttcgtct gctggcgcat
     2161 ctgctgagaa ccctgcaaca cgccggtgat cctcatctcg cattgcgcct gctggcgctg
     2221 tatgtggagc attactggtc ggtggctgcg ccgcagaata tggcacataa aaaacgcttt
     2281 gccagccagg tcattaaacg cttcgaaaca ggtattgaag gcttttcaca gaacgccgcc
     2341 acagcgcagc gcgacgcgct gtcaggggaa ctggcgaaac tggcgcagtg ctggcagtca
     2401 cataacgccc cggaactggc actggctacc gatgatcttt ttgccctgta ccagcgtgcg
     2461 tttaatcgtg cggcaccggc tcctgccccc actccggcgg cctccgatag ttcacgacag
     2521 accaccgcca cgtctgaaag cggcgtgacg caacccagtg ctccggctcc ccaaatcgcc
     2581 atcgacagtc acgacgacaa agcctggcgc gacacgctgt taaaagtggc ggctatttta
     2641 tgtgaacgcc agccggactc gccgcagggc tatcgcctgc gccgccatgc cctgtggcaa
     2701 tccatcacca gtacacccca ggcggaaagc gatggacgta ccccattggc tgcggtctct
     2761 gccgatatgg tggcggatta ccagtcccgg cttgccagcg cggatatggc gctgtggcag
     2821 caggttgaga aaagcgtatt gctggctcct tactggctgg acggtcactg tctttctgca
     2881 cagacggcac tgcgtctggg ttacaaacag gtggcagaca ccatccgcga tgaggtcatc
     2941 cgcttccttg agcgtctgcc ccagcttacc gggctgctgt ttaatgaccg cacaccgttt
     3001 ctcagtgagc agacgaaaca atggctggca gcttcgcccg acggcaaagt tgcaccggtt
     3061 gcgcaaatcg gtgaggaatc gcaggcagcc agagcctgtt ttgctgggca gggtctggag
     3121 gcggcgctgc gatatctgga catgctaccc gaaggcgatc cccgcgatca gtttcaccgc
     3181 cagtaccttg ccgcacagtt gacggaggag gcggggctga tacagcttgc gcagcaacag
     3241 taccggatgt tgttgatgat agggagtcag atgatggtgt ctgactggga gccatcatta
     3301 cttacgcagc ttgaacaaaa attcacggca gaacaataaa agagaacagg agtttttgtg
     3361 ttcagattac caacaccccg actactcagc ggactcaaat cagccctacg cccggcgatg
     3421 ccccggttta aagtctccgc tttctggctg ctgatactgg cgtggttttt tctgctggta
     3481 tggatctggt ggaaaggccc gacgtggacg ctgtatgaag aacagtggct caaaccactg
     3541 gcgaaccgct ggctggcaac ggcggcatgg gggattatcg ccctagtgtg gcttaccgtc
     3601 cgggtgatga agcgcctgca acagcttgaa aaaatgcaaa agcagcagcg cgaggaggcc
     3661 attgatccgc tcagtgtgga gctgaacgcc cagcagcgtt accttgaccg ctggctgctg
     3721 cgcctgcaac gccatctcga caaccgccgt ttcctgtggc agttgccgtg gtatatggtc
     3781 atcggcccgg cgggcagtgg caaaacgaca ctgctgcgcg agggttttcc gtccgacatt
     3841 atttatgccc cggagggcgc acgcggcgca gaacaacgcc tgtacctcac gccccatgtc
     3901 ggtaaacagg cggtgatctt tgatatcgac ggcacactct gcgctcccgc tgatgcggat
     3961 atcctgcatc gccgtctgtg ggaacatgcc ctcggctggc tgaaagaaaa gcgcgcgcgc
     4021 cagccgctga acgggattat tctgacactc gatttacccg atctgcttac cgcagacaaa
     4081 cgccgccgcg aacatctgtt acagacgctg cgcagccgct tgcaggatat acgccagcat
     4141 cttcactgcc agttaccggt ttacgtggta ctcacccggc ttgatttatt gcagggtttt
     4201 gccgccctgt tccagtcact gaacagacag gatcgcgatg caattctagg ggtcacgttc
     4261 acccgccgcg cccatgaaaa tgatgactgg cgaacagagt tgaatgcttt ctggcagaca
     4321 tgggtggatc gaatgaacct agcgttgccg gatctgatgg tcgctcagac tcacacccgc
     4381 gcgtctttat ttagtttttc ccgccagatg cagggaagcc gtgaaccact ggtgtcactg
     4441 cttgagggtc tgcttgacgg cgaaaatatg aacgtgatgc tgcgtggcgt ctatctcacc
     4501 tcttcgcttc agcgcggaca gatggatgat attttcaccc agtctgccgc ccgccaatac
     4561 cggctgggca ataacccact ggcgtcctgg cctctggtgg acaccgcacc ctatttcacc
     4621 cgtagcctgt tcccgcaggc gttactcgca gagcctaatc tggcaacgga gagccgcgcc
     4681 tggctgatgc gttcccgtcg ccgcctgacg gttttctctg ccactggcgg cgtggcggca
     4741 ctgctgctca tcaccggctg gcatcactat tacaatggta actatcagtc cggcatcacc
     4801 gtgcttaagc aggccaaagc ctttatggat gtgccgcctc cgcaggggga agatgactat
     4861 ggcaacctgc aactgccgct gctgaacccg gtacgcgatg ccactctggc ctacggtgac
     4921 tggggcgacc gcagccgcct tgcggagatg ggattatatc aggggcgacg catcgggcct
     4981 tatgtggaac ggacctacct gcaactgctg gagcaacgct atcttccgtc gctgtttaac
     5041 gggctggtca aagagatgaa cgccgcgcca ccggagagtg aggaaaaact cgccgtgctg
     5101 cgcgtgatgc gaatgctgga ggataagagc ggacgtaaca atgaggtggt gaagcagtat
     5161 atggcaaaac gctggagcga aaagttccac ggccagcgcg atatccaggc gcaactgatg
     5221 tcccatcttg actacgcgct ggctcacaca gactggcatg cagagcgtca ggcgggcgac
     5281 ggtgacgcta tcagccgctg gacgccgtat gacaagcccg ttgtgtcagc acagaaagaa
     5341 ctaagcaaac tacctgtcta ccagcgagtt taccagagcc tgaaaacgcg ggcgctgggc
     5401 gttcttcctg ccgacctcaa tctgcgtgac caggtagggc caacctttga ccaggtgttt
     5461 acatctgccg atgacaataa actggttgtt ccacagtttc ttacccgtta cggcctgcaa
     5521 agctattttg taaaacagcg cgatgaactg gttgaactga cggcgatgga ttcctgggta
     5581 ctcaacctca cccgcagcgt gaaatacagt gacgccgacc gggcggaaat ccagcgccag
     5641 ttgaccgagc agtatatcag cgactacacc gccacctggc gggccgggat ggacaatctg
     5701 aatatccgca attttgagtc catcggacaa ctgaccgggg cgctggagca ggttatcagc
     5761 ggtgaccagc ctttgcagcg ggcgctgacc gtgctgcgtg acaacacaca accaggcgcc
     5821 ttttctgaaa agctatctgc taaagaacgg gatgaggccc tggctgaacc agactaccag
     5881 ttactcaccc gccttgggca tgagttcgcc ccggaaaaca gtaccctgac agtccagaaa
     5941 gataaagaaa gcacgatgca ggccgtgtac ctgcagctca ccgagttaca ccgctacctg
     6001 ctggcgatcc agaacgcgcc tgtacctggg aaatcggcac tgaaagccgt gcagttacgg
     6061 cttgatcaga acagcagcga tcccatattc gccacccgcc agatggcaaa aacactgcct
     6121 gctccgctca accgctgggc aggcaggctg gctgaccagg cctggcatgt ggtgatggtg
     6181 gaagccgttc actatatgga agtggactgg cgcgacagcg tggtgaaacc gtttaacgag
     6241 caactggcaa ataactatcc gtttaatcca cattctgcac aggatgcctc actggatgcc
     6301 ttcgaacgat tctttaaacc agatggcata ctggatacct tctaccagca gaacctaaag
     6361 ctgtttatcg acaatgacct gagtctggag gatggcgata acaacgtcat tattcgcgaa
     6421 gatattattg cgcaactgga aaccgcgcag aaaatccgtg acatattctt cagcaaacag
     6481 aacggtctgg gaacatcctt tgccgtggaa acggtatcgc tttccggcaa caaacgccgc
     6541 agcgtactga accttgacgg tcagttagtc gattacagcc agggccgtaa ctataccgcc
     6601 catctggtct ggcctaacaa tatgcgcgaa ggcaacgaaa gcaaactgac gctggttggt
     6661 gcccgcggcg gcgcaccgcg cagtatcagc ttcagcgggc cgtgggctca gttccgcctg
     6721 ttcggggccg gacagctgac cggagtacag gatggcaact ttaccgtgcg ctttagtgtg
     6781 gacggtggcg caatgaccta ccgcgtgcat accgacacgg aagataaccc gttcagcggt
     6841 gggttattca gccagtttgg cctgtcagac acactgtact gaggaggcag ggatgaatag
     6901 taacggactg acacaaacta tcgttaccgg cagtgacccg cgcgggctgc cggaattcag
     6961 cgccatccgc gaggaaataa acaaagccag ccatccgtca cagcctgagc tgaactggaa
     7021 actggtggag tcgctggcgc tggcgatttt taaagccaac ggtgtggatt tacacaccgc
     7081 cacctactat acgcttgccc ggacacggac acagggactg gcgggattct gcgaaggtgc
     7141 ggaactgctg gcggcaatgg taagccacga ctgggataaa ttctggccgc agggcggtcc
     7201 ggcgcgtact gaaatgctgg actggtttaa ctcccgaacc ggcaatattc tgcgtcagca
     7261 aatctccttt gcggaacccg atctgccact gatctaccgc acagagcggg cattgcagct
     7321 tatctgcgac aagcttcagc aggtggagct gaagcgcgtt ccgcgcgtgg agaatctgct
     7381 ctattttatg cagaacacgc gtaagcggct tgaaccccag ccgaagagta acgttgaaaa
     7441 cgccgtacag accacggtta gaacactgat ttatgccccg gaaacaacgg cttcctccac
     7501 gccagaagcg gtagtgcctc ccctgcccga cctgcctgag atgaaagtag aagtgcgcag
     7561 tctgtcagag aatcccccac aggccagtgt gatgaagcaa ggcagtacgg taagagggtt
     7621 tatcacgggg atcgcctgtt cagtggctgt cgcctcagca ttgtggtggt ggcaggtcta
     7681 tccggtgcaa cagcaactgt tacaggttaa cgacaccgcg cagggcgcag caacggtgtg
     7741 gatggcctca cctgaactag aaaactatga gcgcaggctg caacagcttc ttggcgtctc
     7801 cccggtacaa ccgctggaaa ccgggatgca gatgatgcgt gttgccgaca gtcgctggcc
     7861 ggaaagcctg cagcagcagc aggcctcgac acattggaat gaagcactca aaacccgcgc
     7921 acagagtagc ccgcagttgc gtggctggtt gcagacccgt caggacttac atgcttttgc
     7981 agatctcgtg atgcggcgcg agaaagaggg actaaccctt tcctatatca aaaatgtcat
     8041 ctggcaggcg gagcggggac tggggcagga aacacccgtt gagtctctgt tgacgcagta
     8101 ccaggatgcc cgtgcgcaga agcagaatat agatgcgctg gaaaaacaaa ttaatgagcg
     8161 actcgaaggc gtgttaatcc gctggctgct gctgaagaat aacgtcatgc cagaggcggc
     8221 aaccgggacc acggccgaaa aataattatt taattaaagg agattatcat ggcaaatatg
     8281 agttatttat ctttgagtgg agagacacaa gggctgattt cagcaggttg ctcaacgctt
     8341 gattctgtcg gaaacaaagc acagccagaa cataaagatc agatcatggt ttatgcgtta
     8401 atgcattcca tctcaagaag tcaaaatgtc aatcatcatg aattaattat caccaaaccg
     8461 gttgataaat cctcaccatt gctggcaaaa gcacttagtg acaatgaaaa aatggtaaca
     8521 tgtgaattta tactctaccg aaccagtaaa gcgggtattt accagcctta ttataaaata
     8581 aacctgtcaa aagcccgaat atcaagtatt gattttgtca caccgcatgc tgtcctggaa
     8641 aaagaactgg agccacagga gagaattgcc tttatttatg aagatatttc ctgggagcat
     8701 acgttagccg gtacgaatgc aatgagtaaa tggcaggatc gtgtacagta atttactgta
     8761 ataacataat aaattactat tgcggagaga tataatggct ggtttaagga ataataacaa
     8821 cacacagaat gcacagtggg ctgattatgt gggtgacatt cttcgggggg ctcaaccgat
     8881 aaaccagcta gtacctcagc acccttatct taacgacgtc ccactcattg atgaattaag
     8941 gcgtcagaac actcatcatg ttattctctt gacacttgat gttgcaaaaa aaatactcag
     9001 ctcaataaca tcatttgatt acattcattt tatcactaca catccttcag gtataaaaga
     9061 tacactcgct tggttagtta acgcgggtaa attaatgact gagtttgatg gaaatgggaa
     9121 aattatattt aactttaata ccttaaagta tacaaaagca agttactttg aaatacttgg
     9181 agagaaatat ataaaaatta caacatccag cccctggctt ttagaaaagc tcggtaaata
     9241 tattttctct tcacgagcgc ctcaagttct ggaactcgct attggctggc gaggagcttt
     9301 atctgaatca ataaaaggag ttaagttctg tatctggttt tcagtagcct ggagaaccat
     9361 agaatttatt atgagttcag agcgagatct ggttaatttt cttggtgatt tttcaatgga
     9421 tgtggccaaa gcagtgattg caggcggtgt tgcaactgct attggaagcc tggcttcttt
     9481 tgcctgtctt agttttggcc ttccagtaat tcttgtagga ggagcaattt tacttacagg
     9541 gatagtgtgt acagttgttt taaatgaaat cgatgctcaa taccatttat cagaaaaatt
     9601 aaaagatgca attagagatg gactaaaacg gcaacaggaa cttgataaat ggaaaaggga
     9661 aaacatgact ccatttatgt atgttcttaa cactcccccc gtgatatgag gattaactat
     9721 gcgtttacat gttaaattga aagagttttt ttcgatgttt tttatagcca tattattttt
     9781 ccctgcattc aatgcttctt tattttttac cggaattaaa cctctatatt caataataaa
     9841 aggctccacg gaaatatttt atgattggag aatgttgatt ctatgcttcg gtttcatatc
     9901 gttttccttg ttaaatattc atgtaatttt actaacaata ataaaatcat tcttaataaa
     9961 aaaaacaaag gtaatcaatt tcgccacaga cataacaata cagctaactc ttatattttt
    10021 actaatagct atcgtaatag cacctttaat agctcctttt gtcacaggat atgtcaatgc
    10081 taattatcac ccctgcggta ataacacggg gattttccca ggagcaatct atattaaaaa
    10141 tagcataaaa tgtaataatg aacatatttc tcacaaggaa gattaagcag ttaaataatt
    10201 aaatattcta aaacaaacag tttttaacgc cggattccat gtcctttctt gcactgtctt
    10261 tgtcaggaac aatttcttat gcaaagaatt ttgtattatt gccgccagaa attcctggcg
    10321 gcagatataa tttattattg atagcggtta accaaatctt ttaccatcgc tttataggtc
    10381 ttttgcttaa gttgtatgat ttgctgctct gtcatatcag tcgtactggc ttcattcttg
    10441 ccgggaaatg gtggaagcac tttatattca taattcaccg gcgaccaggc agcccactca
    10501 cctgtagcta catccaccag cgtgaatcgc accagatagc gcagtgagat tgtgttagtc
    10561 agtttttcat ttttataatc ggaccatact gttgatttca gtgcagtgtc atattttcct
    10621 gtctgcaacg tatcctggta aacaataatc gctttctgat ggccttttgc cgccacaaaa
    10681 cgtaatgcct gcatccagtt catattttca gcactggcaa catcctcgtt ctcattttta
    10741 tctttgtttt tgttacaagt cagaggcttt tgtctgtccg ggataccaga gaatgtggaa
    10801 acagtataat attttcgcat ctcctcctgc ataatggttt ccggtgcgcg gttgccggat
    10861 tgaacaagta ttacaggtga attaagggga acgcggaact catctccatc aagcgcagct
    10921 tgaatatcct cctcggatac ggctagtgtg gtttcattac caaataagtc tttgtctgtt
    10981 agttgctgcg gagcgttttt attaaaatta ctttgtgtgg tttgagcttt tttcttcttt
    11041 tcatcaataa gagattcaga gacacatcct gcaagcagcg agaataatac atatatacaa
    11101 caaacctttt ttgaattcaa aatcggacac tccctcgtct gtcataatca tggtaatgtt
    11161 tagctaacga tttttaatta tata
//
LOCUS       DAPOZO010000136        11075 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPOZO010000136 DAPOZO010000000
VERSION     DAPOZO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.136"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..394)
                     /locus_tag="ROI21_004238"
     CDS             complement(<1..394)
                     /locus_tag="ROI21_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     ubiquinone; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDX5113090.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLV"
     gene            complement(391..693)
                     /gene="nuoK"
                     /locus_tag="ROI21_004239"
     CDS             complement(391..693)
                     /gene="nuoK"
                     /locus_tag="ROI21_004239"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDX5113091.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(690..1244)
                     /gene="nuoJ"
                     /locus_tag="ROI21_004240"
     CDS             complement(690..1244)
                     /gene="nuoJ"
                     /locus_tag="ROI21_004240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDX5113092.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(1256..1798)
                     /gene="nuoI"
                     /locus_tag="ROI21_004241"
     CDS             complement(1256..1798)
                     /gene="nuoI"
                     /locus_tag="ROI21_004241"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDX5113093.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(1813..2790)
                     /gene="nuoH"
                     /locus_tag="ROI21_004242"
     CDS             complement(1813..2790)
                     /gene="nuoH"
                     /locus_tag="ROI21_004242"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDX5113094.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(2787..5513)
                     /gene="nuoG"
                     /locus_tag="ROI21_004243"
     CDS             complement(2787..5513)
                     /gene="nuoG"
                     /locus_tag="ROI21_004243"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDX5113095.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(5566..6903)
                     /gene="nuoF"
                     /locus_tag="ROI21_004244"
     CDS             complement(5566..6903)
                     /gene="nuoF"
                     /locus_tag="ROI21_004244"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDX5113096.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(6900..7400)
                     /gene="nuoE"
                     /locus_tag="ROI21_004245"
     CDS             complement(6900..7400)
                     /gene="nuoE"
                     /locus_tag="ROI21_004245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDX5113097.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(7403..9205)
                     /gene="nuoC"
                     /locus_tag="ROI21_004246"
     CDS             complement(7403..9205)
                     /gene="nuoC"
                     /locus_tag="ROI21_004246"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDX5113098.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(9299..9961)
                     /gene="nuoB"
                     /locus_tag="ROI21_004247"
     CDS             complement(9299..9961)
                     /gene="nuoB"
                     /locus_tag="ROI21_004247"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDX5113099.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(9977..10420)
                     /gene="nuoA"
                     /locus_tag="ROI21_004248"
     CDS             complement(9977..10420)
                     /gene="nuoA"
                     /locus_tag="ROI21_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDX5113100.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
BASE COUNT         2609 a         3154 c         2857 g         2455 t
ORIGIN      
        1 gcaccagaac taccatgctg gcgatgaaca ggttggtgta agcgaagaag cgagagtagc
       61 cctcttcacc gcgcatatac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
      121 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
      181 cacctaccga catccacgtc cacagcggct ggctatatgc ctgctcgccg ttagcgaaga
      241 agtcaacgcc gataaaagcg gttaccagcg ccgccaagcc cacagagcca acgcccacga
      301 tcgccgagac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
      361 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
      421 gttcaggttc tggcgacgac ggtgaagttg cagcagcagt gcaaggccga tacttgcttc
      481 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct ggccccagta
      541 gcttccggcg accacaaagg ccagcgcaga ggcgttaatc atgatttcca gaccaatcag
      601 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
      661 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
      721 ctgtcgtctt tacgattgct cagcacttca cccgctcgct cttcacgacc aacgtggaag
      781 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
      841 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
      901 ttaacaccaa gaatggcgta aacaatcacc accagcatga tggccgacaa aattgccgga
      961 ccaatccaca cctgcggttt cagccactgg cgttcctgtt caatttctga accgcccagg
     1021 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacaataatt
     1081 tccagcgcac cggcaaagta agcgcctagc gagaagaaca ccccggaaat cgccagcagc
     1141 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     1201 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     1261 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     1321 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     1381 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     1441 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     1501 tgcagcgtga gaagttgatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     1561 ctttttgcag cgagatacag ccgaccgggc aggctaccgc gcagaggtta caggctacgc
     1621 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     1681 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgca tgcaggccga
     1741 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     1801 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     1861 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acctggtcat aacgcggacg
     1921 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     1981 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcag
     2041 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     2101 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     2161 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     2221 gatgacgttc cacacatgcg cctggctgtt aacgatgtcg gtcatgttga atgaaccggc
     2281 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gagtctgcgc
     2341 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     2401 caccgcgtag accgccagac cggccatcat caggaagaac aaaatcccga tgttcaggtc
     2461 ggcaaccacc caacctgggc tgactggcac aatcgcaaag gccagcagca gcgaggtaaa
     2521 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     2581 tttaaagaac atcttgatca tgtccgcgac cagctggagc gaaccgcccc agccaacacg
     2641 gttaggtccg taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     2701 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     2761 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     2821 gcgcccgcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     2881 gtcagccctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     2941 acgcgtgtac ccgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     3001 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     3061 aggtggtaat acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     3121 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     3181 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     3241 cccggtgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     3301 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgatg
     3361 ttggcgcgca tggcggtacg accgctgtaa cggtgtggtt cacgggccag tttctgtcca
     3421 cggatacgga atgtcgcatc cggcgcagca tctttgatac ctgccagctc cgggattttc
     3481 gccacaacag cgtcaatcac atggtcgagc tgcgtccagt ccacttcacg gctcagcagg
     3541 gtgctgtgca gcgagtgtaa ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     3601 taggccggat cgtaaacctg gaagaaacgt tgggcgcggc cttcgttgtt gatcaccgta
     3661 ccgtcgcttt cagcaaagct ggcagcagaa agcaccagat gggcgttttc cataatcgcc
     3721 gtgcgttgat ggtcaaccac catcaccagc ggcgctttcg ccagcgcagc attcacgcgg
     3781 gtagcggaag cgtgacgatg cagatcgttt tccagcacca ccaccgcgtc ggcgcgtccg
     3841 gtttccagtt cggttaacgc ttcttcaagt gatccaccgc ccataatgcc cagccccata
     3901 ctgtttacag agcgagcaat catggtgata ccgacgtcag cgccgcgacc tttcagggct
     3961 ttcgccacgt tagccgcagc ctgaatcact tcagcgctac cagcgttcgt cccggagata
     4021 atcaacggtt tcttcgcacc cgccagagcc tgcacgatga cgtcgatttt gctttgcagc
     4081 tcaggttcga ttccgtcaac cgctggcgcg gagttatcca gcgcatgggc gatggcaaaa
     4141 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
     4201 cgggtatcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
     4261 attgccgcaa tctgccagtc agccactttc tgtgctgccg ccatttcgcg cgctttacct
     4321 ttcaccgcct gacgcactgc cagcgcgacg cgcgcaccgg tctgggtaac gtcttcgccc
     4381 agcaccagta ccgcatcgta agattcgatt tcgcgcagtg ccggcgtata aatgccgcct
     4441 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
     4501 tagaagtttt cttcgcccac cagctcacgc agcgcaaagt tgctttccac gctggcacgc
     4561 ggagaaccga tgccgatcac tttcttcgac tgacgcagaa tatctgctgc gccctgcatt
     4621 gcctgttcgg cattgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
     4681 cgatctttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
     4741 tttactgtac cgttgtaacg gttttcgatt cgacgcagtt cgccataacg ctcgccgggg
     4801 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
     4861 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
     4921 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
     4981 ttgtcgtgtg caccgtaaac gcccaggtcc gtgccgtcag cgtaatcttt gtagtaacgc
     5041 acgcagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
     5101 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
     5161 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacaatc gtgcgggtgg
     5221 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc atcgtcaatg
     5281 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
     5341 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
     5401 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
     5461 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
     5521 cttccagttt tctcagtcag agactgagcg ttaatcgaaa ttcggttacc agcgctcttt
     5581 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
     5641 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
     5701 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
     5761 gatatcgccc ggctgacctt cgccacgctc cagcgcgcgc agaattttca cgctccacgg
     5821 cagaccgtcg cggcacggcg tacaccagcc acaggactca cgggcgaaaa actcttccag
     5881 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
     5941 cagacggctt cccgctttac cgatactttc gaattccatc ggcaggtcaa ggtgcgcttc
     6001 agtcaggaag tcagtacccg caccgcctgg ctgccaggct ttgaatttca gaccgtcacg
     6061 cataccaccg gcgtaatctt cgaggatctc gcgagcggtg gtgccgaacg gcagttccca
     6121 caggcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
     6181 tttcgagatg ttctgatacc actccacgcc gttagcaagg atcgccggaa cgttacacag
     6241 ggtttcgacg ttattgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
     6301 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
     6361 cccgcagatg tagcgccctg caccggtatg gacgaacagt tcgaagtcga aacctgttcc
     6421 cataatgttt ttgccaagca agcccgcttc agtcgcttcg gcaatggcgc ggcgcagatt
     6481 cactgccgct tcaatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
     6541 aaacgcggag ataagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
     6601 gtctttatag gtgcccggtt ccatttcatc ggcgttacac agcaggtaac ggatgttcat
     6661 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
     6721 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
     6781 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
     6841 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
     6901 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
     6961 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
     7021 gtaaagcgac cgtcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
     7081 gcctgaatac cctgataacc gttgatatga cagaccacgc tgtcgcaata acggatcaca
     7141 tggcgaccaa ccggctggcg gaagatctgg ctgtagaacg ttgccacgcc ttctacgtcg
     7201 ctcgccggaa tacccagcac atcggcgatc gcgtggatcg cgccatccgg cacccagcca
     7261 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
     7321 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcagtttgt
     7381 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
     7441 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
     7501 aatgcgcata actcggcgta cggatacggg tgcgataact catggtgcta ccgtcgctgg
     7561 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggctg
     7621 gcatcaccgg accccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
     7681 acgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
     7741 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
     7801 gcattacgcg ggtgtagcaa tcagaaacac caccaccaac cgggatttca aagtcgaagt
     7861 tttcatagcc agaatacgga cgcgccttac gcacgtcgaa gtcgatcccg gtggcgcgca
     7921 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
     7981 gggaacgacc tttcagaata gtgttttgca gcgccgcttt ctcgtaagac gccagacgtt
     8041 tcggcatcca gtcgaggaac tcacgcagca ggcgatccca gccacgcggc aggtcgtgcg
     8101 ctacgccgcc aatacggaac cacgccgggt gcatacggaa accagtgatt gcttccacca
     8161 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac tggcgtcatt gcgccgacgt
     8221 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat gcggaacagc tcagaaagca
     8281 ttacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
     8341 gcacgtaagg catttcgtta acgcagccac cgaggtattc gatacggtcg gtatacggaa
     8401 tgtagctatg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
     8461 tatctggtac gcagtcgaca atctcttcgc catcgagctg caaaacgata cggaaagcac
     8521 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc
     8581 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
     8641 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca
     8701 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
     8761 aagtaatgcc aaacagatcc caggtctcac gctcatacca gttagcgttc gggaacagtt
     8821 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcaac atgatgtcgc
     8881 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
     8941 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
     9001 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
     9061 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
     9121 gttcgccaat caccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
     9181 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
     9241 cttcgcgaaa tagatttagg aataagcgaa gaaaaattcc tttgccgaca ggcgcaaatt
     9301 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
     9361 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
     9421 gacgttcttt accgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
     9481 gcggagggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
     9541 cggaataaat atcgtacata ccgccagagt tggcacaggc acccattgag ataacccatt
     9601 ttggctccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
     9661 ttcctgccac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
     9721 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
     9781 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
     9841 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
     9901 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
     9961 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    10021 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    10081 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    10141 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    10201 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    10261 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    10321 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    10381 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    10441 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    10501 aaaaaaaacc ttacaatcac tgtagaaatt cttttataca gctaattgat gtggtctttt
    10561 actcctttct ataacctttt gtcaacttta acaaaagttt cttcacatta gtttacataa
    10621 tatcaacacc attagcattt aatgcccttt caccccagat ccttgacgac tccaggataa
    10681 ttagatgttg ttgaatcgtg tccgttgtga agcaatggaa aaaatacggg cctattttga
    10741 caggaatttg tgtcgattcc tccccccaaa agagagtatt ttcttgatct gtgacacgct
    10801 tttgtcattc cataacaaaa acgcagcaac aaatttacgt attttttaac atcattgtag
    10861 caggtgattt ttttcaggcg attatttgtg cgttcgggac ttgaatctct ggtgattgaa
    10921 aaatgaacag ttttgtacgt tctgcacgat acgaggaagg ctttttacta aaaaaaagcc
    10981 gctggggttt aaaacacccc cagcggctcg tttttacact attgtctcag aaattatcta
    11041 tggtccgtcg attactcgat atccctttca atcaa
//
LOCUS       DAPOZO010000137        11009 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPOZO010000137 DAPOZO010000000
VERSION     DAPOZO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.137"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            96..254
                     /locus_tag="ROI21_004249"
     CDS             96..254
                     /locus_tag="ROI21_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDX5113101.1"
                     /translation="METLSTNLQLARLVGVQGTPATIIGDEMIPGAVSWETLEAVVKE
                     KLAVAHAQ"
     gene            244..750
                     /locus_tag="ROI21_004250"
     CDS             244..750
                     /locus_tag="ROI21_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003846856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDX5113102.1"
                     /translation="MLSKLRRWLREGAILLVVLAGVIILLDVWRSPQMPAMFDSTPLH
                     TLDGETVTLASISEERPVLLYFWASWCGICRFTTPDVARLQSEGESVMTIALRSGNDG
                     EVSRWLSRKRVTFPVVNDSGGEISRNWEISVTPTLVVVSKGQVVTTTSGWTSYWGMKL
                     RLWRAAMF"
     gene            933..1748
                     /locus_tag="ROI21_004251"
     CDS             933..1748
                     /locus_tag="ROI21_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDX5113103.1"
                     /translation="MIRSGDFFDFHFDRDNNSMRKGKIIVASMMVLCVLIFVAAAAWF
                     MLFQGEMTEPTEEEKQAAILHAAGLMKSETQDKKSVETLYHRYIIQRHVNLDSGELVA
                     GSSADTARQKCEKLAPERDSAQVRQRCTVADVFFVKDKNNEIQQVIIPVTGKGAKSMM
                     HAFLALGLDGRTVRNLYYYQQRETPFLGARVEDANWRKQWPGKRLLDNSGHPALKIVQ
                     DKPEHADEYTVDGISGATLTSTGVEKSINYWMGPQGYGQFLQRLASDRNNLNL"
     gene            2095..3981
                     /locus_tag="ROI21_004252"
     CDS             2095..3981
                     /locus_tag="ROI21_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HDX5113104.1"
                     /translation="MSVWASTNYAPLIEDIEQRLDKTAELYQQQHADEARRTVQMAYF
                     EVFENLEGPIRINISARKSYEMESAFGEIRRMIGEKKPLADVQARIDWLKAALREVEP
                     VLDGGHRLVAEEQHNALSRDDIAVHWQESFRTIDDLLAQAVTEYQAGNYSVASQHVQQ
                     AHYQGFKNSEMEMSLRQNRSAKDAASINQQFSSLIALTAQPDHLNDVSYQVTTLLQDI
                     EDILPGLPTTRDDQQVAAAQSADNIPAADEGNNRTDWAEVTTGINQSIQDAIARYQGG
                     DAKNAILDIQDTYFDRFEASGMENKIGSRDSAFKTTLEAYFTRLVSLMKAGQPVERLN
                     AEASALTQDLQKAVTMLGEGEETQWSLLLYSLMIIVREGLEALLIVAAIVAYMVKNNH
                     QDKLPLIRQSVIVALIASVITAAIFQMLFTNSGASRELLEGITMLIAVVMLFFMSYWL
                     LSKVEARHWKAWLEGKLSHSLSRGSLVGLWLTSFLAVYREGAETVLFYYALIGDANTV
                     SGHMAIGAGFVIGCVVLLLAWLVMRYSVVRLPLKPFFMFTGSFMYLMAFVFAGKGVLE
                     LVEGKLFQPTLINGFPEISWLGIYPYVETLLPQVVLLLAALVALWVMRRKSAVPGETI
                     KNNP"
     gene            4022..4549
                     /locus_tag="ROI21_004253"
     CDS             4022..4549
                     /locus_tag="ROI21_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016674077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDX5113105.1"
                     /translation="MTMKKTLIASAVMASIFIAPAAFAFKEYPAGEPVTMNEMELAAV
                     YLQPIDMEPRGMGLPAAKADVHLEADIHAVEGNKNGFGAGEWIPYLTISYTLVNNDTG
                     EKQEGTFMPMVASDGPHYGANIKMMGVGNYKVTYHIEPPSKAGMHRHTDSETGVGRWW
                     KPFDVSYEFKYVGLN"
     gene            4653..6032
                     /locus_tag="ROI21_004254"
     CDS             4653..6032
                     /locus_tag="ROI21_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HDX5113106.1"
                     /translation="MSYFFVTTLQVFFCIALLSGVLWSRNDPPSLRPLTWTLLTGLIA
                     GVLAGLFIHGSQPVQLLLVGAEVMVSLLFVLSFWWASTRIRYLWQGILIFGAARHWAL
                     DPNLGGLTSTHVLNTDLLLNLTAVVLAFAILCLAGVLCAMLLRRIRGLYWPLTLILLV
                     MIWLPLSGNLLLLLMKLQVVPLGKSLLSFVAKVTNNTALYNWAGAALLLALALCWLPA
                     LLRAFRQTRETEEPIAHRLALAQRRNALRLWLVTIGCAVVVIAGQLWWDKVASQPPQL
                     SEAVPVTLGSDGMVRLPVEQLRDGKLHRFVWVADDGKAVRFFVINRYPDKLRFGVVFD
                     ACLLCGDQGYVMEGNQVICVACGVHIFIPSIGKAGGCNPVPIENWHNDEKELVIPGKE
                     LATGVNYFSTVMTIKVTDPVDGSTLTNTSADYKYSYGGKTWFFSSEANYERFRETPEQ
                     FVPADMREE"
     gene            6035..7318
                     /locus_tag="ROI21_004255"
     CDS             6035..7318
                     /locus_tag="ROI21_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5113107.1"
                     /translation="MLWRMLRQSWGRNLRRKVLAIITVFLASSLISALLAVSIDIGDK
                     MSRELKSYGANILIEPAGQAALPALFSESSNPLSGQDFLDEAELPNIKDIFWRNNIVG
                     FAPMLGGEASVEGEPVRILGTFFSQPVDIPDEEGYETGQKTVSPYWQVTGDWPQEPAG
                     AEPQTLVGHALARQMGWKPGDKLTLRTEGEAVQVTVSGILSSGGDEDNQLVMPLSTVQ
                     HLLGLPGKVQAIRVSALTVPENELSRRARENLDALNAEEYDLWYCTAYVSSIAHQLEE
                     AISGAEVRPVWQVAASEGVVIDKIQLLMAVVTVAALVASAMGIASLMTSTIMERAKEI
                     GLMKALGARQWQIMLLFYLEAASSGLAGGALGCIAGWGLAKAIGVMLFDAPLNFAWIV
                     VPCVLVIAVLIALIGTWFPARRIARLYPVEVLYGR"
     gene            7308..8438
                     /locus_tag="ROI21_004256"
     CDS             7308..8438
                     /locus_tag="ROI21_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDX5113108.1"
                     /translation="MAAKRTMLWLLVWRALRLRFQRVSVVFAALMVGATIVTALFAVW
                     FDINTKMSEELRTFGANFYIGPGHGSSMPQQELQSILDQAPQGLVHGASPWLYGMART
                     ELEKVVMVGVWFESLQKLVPYWQVTGSWIGVSFDDRNAMIGVKLAERLNVQPGDSITL
                     VDHNQRKNLQIKGIVESGDATDNMLIVSLDVAQAWLHQPGKISHGLLSVSNDVGQVEN
                     YASRLQAQYPDLEIRPVRKVSASEGQVLDKIKGLMGLVSIVILALSSLCVNTTLMAIV
                     GERAREFALQKALGSSNGDIVRQILLETSIIALAAVACGWVLGYLLAQLLGLTVFNAA
                     ISLRLPVLPITLVLSLLVAILAAIVPVRRAVSVEPAKVLKGE"
     gene            8443..9138
                     /locus_tag="ROI21_004257"
     CDS             8443..9138
                     /locus_tag="ROI21_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004863043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5113109.1"
                     /translation="MSVTHIPQVAIETRHLYKRFGDVTALDDINLRIAQGEFVAIMGA
                     SGSGKTTLMNILTCLDTATEGQVFLDGTDAAALDEEGRRRFRAEKIGLVFQQFHLIPF
                     LTALENIMLAQHYHSVVDEAAARKVLEQVGLGHRVTHLPSQLSGGEQQRVCIARALVN
                     EPPVIFADEPTGNLDEENEQRVLDLLTDLHRQGRTIVMVTHNPALGQFADRILRLQHG
                     KYLGEEANQHALA"
     gene            9125..9610
                     /locus_tag="ROI21_004258"
     CDS             9125..9610
                     /locus_tag="ROI21_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDX5113110.1"
                     /translation="MRWHNVLLLAAAILISGCKEEKLAVGAPAPALAAFDLQGQESGL
                     ERWQGKAVYLNFWSAGCGGCLAEMDTLEALSKKWGDKVVVVAVNTDPETVRINDLLAK
                     HQITYPVVRDQLNITQERYQVIGTPTSVLIDSEGRVLELHQGMRKPSELEATFARLAA
                     R"
     gene            9635..10120
                     /locus_tag="ROI21_004259"
     CDS             9635..10120
                     /locus_tag="ROI21_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113111.1"
                     /translation="MAFIWNDESLAILRENAGILTTEQIAQLLHTNITAVRNMAYRLK
                     LSLRVTAYNHRRIAQVQALYASETLSLKEIAAKTGLTASTVQYIVYVKSKNKPYATTE
                     YVSFETENAVHYRVQKEFVDTERSLLDNISDNTRFRELYLTDGTFYCARNIKYEVFIS
                     E"
     gene            10242..10875
                     /locus_tag="ROI21_004260"
                     /pseudo
     CDS             10242..10875
                     /locus_tag="ROI21_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
BASE COUNT         2432 a         2736 c         3315 g         2526 t
ORIGIN      
        1 gcagacagaa accgtaaaca ccggcattga catgccgggg aagcccttat agtacaataa
       61 ttctctatat ccaaactgac cccgatgaga aaagcatgga gacgttgagc accaatctgc
      121 aactcgcccg gctggttggc gttcagggga cgccggcaac catcattggc gacgagatga
      181 tccccggcgc ggtatcgtgg gagacccttg aggcggtggt gaaagaaaaa ctggcggtgg
      241 cccatgctca gtaaactgcg ccgctggctg cgtgaaggcg cgatcttact ggttgtgctg
      301 gctggcgtga taatcctgct ggatgtctgg cgttctccgc agatgccggc catgtttgac
      361 agcacgccgc tgcatacgct ggacggggaa accgtaacgc tggcgtcgat cagtgaagaa
      421 cgcccggtgc tgctctactt ctgggcgagc tggtgcggta tctgccgttt caccacgccg
      481 gatgtcgccc gtttgcagtc ggaaggggag agcgtcatga ccatcgcgct gcgttccggt
      541 aatgacgggg aggtttcccg ctggttatcg cgtaaaaggg tgacgttccc cgtcgtgaat
      601 gatagcggcg gtgagatatc acgaaactgg gaaatcagcg tcacgccaac gctggtggtg
      661 gtgtcgaagg ggcaggtggt aactacgacc agcggctgga ccagctactg gggcatgaag
      721 ttacgtctgt ggcgggcagc catgttttga tagcgtgccg gggaaacccg gcatttttta
      781 acccattgta aggatgtgta aaacgagcgg agagcgtgtt ttctgttatt tttgctgctt
      841 ttttaccgac gccagttatt attttttacc gctgcctgac gttgcaaacc tgaattgata
      901 atggcatatc tgttttaatt gaatgaaagt ctgtgatacg ttcaggcgat tttttcgatt
      961 tccattttga cagggataac aattcaatgc gcaagggtaa aattattgtt gcatcaatga
     1021 tggtgctttg tgtccttatt tttgttgcgg cggcggcatg gtttatgctg ttccagggag
     1081 aaatgacgga gccgacagag gaggaaaaac aggcggcaat tttacacgct gcgggcctga
     1141 tgaaatcaga gacccaggat aaaaaatcag tcgaaacgct ctatcatcgt tatattattc
     1201 aacgccatgt taatttagat tccggcgaac tggtggctgg cagcagcgcg gacaccgcgc
     1261 ggcagaagtg tgaaaaactt gcccctgaac gcgactcagc gcaggttcgt cagcgctgta
     1321 cggtggcgga tgtgttcttt gtgaaagata aaaataatga aattcagcag gttatcattc
     1381 cagttaccgg taagggtgcg aaatccatga tgcatgcgtt tcttgctctg gggctggacg
     1441 gtcgtacggt gagaaatctg tattattatc agcaacggga aacgccgttt ctgggggcta
     1501 gggtggaaga cgccaactgg cgcaaacagt ggccagggaa acgattatta gataacagcg
     1561 gacatcctgc attgaaaata gtacaggata aacctgaaca tgccgatgaa tataccgttg
     1621 acggtatttc cggtgcgacg ttgacatcaa ccggcgttga gaaaagtatt aactactgga
     1681 tggggccgca gggatatggt caattcttac agcgtcttgc cagcgatcga aataacctta
     1741 atctttgaat attgctgtac gcaataacgc gttttatttc tgcctgccga taatgaataa
     1801 tatcactgtc ggcaggggga tttattcctc tgttaccgcg cctgccttgc cgttaattcc
     1861 atgagaaaaa cagggagata acgtcccttt tatcaacgca taatgtgtgt gttcatcacg
     1921 atttgatgaa ataagtttac ataacttgtc gtctgctgac atttttatga tccaggtcat
     1981 gtataacgcg acaaaaagat atgataataa ttatcatttg tattctttgg cggtgtgcac
     2041 gtgcgtaact tccgtaactc tttgttggtt actctctttg gcttgtttat aagcatgagt
     2101 gtctgggcgt cgacgaatta tgcgcctttg attgaagata tcgagcaacg tcttgataaa
     2161 acagcggaac tttatcagca acagcatgcc gacgaggcgc gccgcacggt acaaatggcc
     2221 tattttgagg tgttcgagaa cctggaaggg ccgatccgca tcaacatttc cgcgcgcaaa
     2281 agttacgaga tggagagcgc attcggcgag atccgtcgaa tgattggcga gaaaaaaccg
     2341 ctcgctgacg tccaggcgcg cattgactgg ctgaaagccg cactgcgcga agtggaaccg
     2401 gtgctggatg gcggacatcg tctggtggcc gaagagcagc acaacgcgct gtccagggac
     2461 gacattgccg ttcactggca ggagagtttc aggacgatcg acgatctgct ggcgcaggcg
     2521 gtcactgaat atcaggccgg gaattacagc gttgccagtc agcacgttca acaggcgcac
     2581 tatcagggct ttaaaaactc tgagatggag atgtccctca gacaaaaccg ttctgcaaaa
     2641 gatgccgcct ccattaacca acaattctca tccctgattg cgctgaccgc gcaacctgac
     2701 catctcaacg atgtctccta tcaggtcact acgctgctac aggatatcga ggatatctta
     2761 cccggcttgc caaccacccg tgacgatcag caggttgccg cagcgcaaag cgcagacaac
     2821 attccggcgg cggacgaggg aaataaccgc accgactggg ctgaggtgac gacgggaatt
     2881 aaccagagca ttcaggacgc gatagcgcgc tatcagggcg gcgatgcgaa gaatgcgatt
     2941 ctggatatcc aggataccta cttcgaccgc ttcgaagcca gcggcatgga aaacaaaatc
     3001 ggctctcgcg attcggcatt taaaaccacg ctggaggcgt attttacccg cctggtcagt
     3061 ctgatgaagg ccggtcagcc ggttgagcgc ctgaatgcag aggccagcgc gctgacgcag
     3121 gatctgcaaa aagccgtgac gatgctgggc gaaggcgaag aaacccagtg gagtctgctg
     3181 ctctatagtc tgatgatcat cgtgcgcgag gggctggaag cgctgctgat tgttgcggca
     3241 atcgtggcct acatggtgaa aaacaaccat caggataagc tgccgcttat tcgtcagtcg
     3301 gtaattgtgg cgctgatcgc cagcgtcatc accgccgcta tcttccagat gctgtttact
     3361 aactccggcg ccagccgtga actgctggaa ggcataacga tgctgattgc cgtcgtgatg
     3421 ctgttcttta tgagttactg gctgctgtcc aaagtggaag cgcgccactg gaaagcctgg
     3481 ctggaaggca aactgtctca ttccctgtcg cgaggctccc tggtcggact atggctgacc
     3541 agcttcctgg cggtgtaccg cgaaggggcg gaaacggtgc tgttctatta cgctctgatt
     3601 ggcgatgcaa acacggtatc cggccatatg gcgattggcg ccggttttgt gattggctgc
     3661 gtggtgctgc tgctcgcctg gctggtgatg cgttactcag tggtgcgcct gccgctgaaa
     3721 cccttcttta tgtttaccgg cagtttcatg tatctgatgg ccttcgtctt tgcgggcaaa
     3781 ggcgttctgg aactggtgga aggcaaactg ttccagccta cgttgattaa cggcttccct
     3841 gaaatcagct ggctggggat ttatccctac gtggaaacgt tgttaccgca ggtcgtattg
     3901 ctgctcgcgg cgctggtggc gctgtgggtg atgcggcgca aaagcgcggt tcccggggag
     3961 acgataaaaa ataacccata atcgttgtat agccgttgtt ttagacgaga ggatacgttt
     4021 gatgaccatg aagaaaaccc tgattgccag cgcagtgatg gccagcattt tcattgcgcc
     4081 agccgcgttt gcttttaaag agtacccggc aggcgagcct gtcaccatga atgaaatgga
     4141 gctggcggcc gtttacctgc aaccgattga tatggaaccg cgcggcatgg gcttaccggc
     4201 agcaaaagcc gatgttcacc ttgaagcgga tatccacgct gtagaaggca ataaaaacgg
     4261 ctttggcgca ggggaatgga tcccgtacct gaccatcagc tacaccctgg tcaacaacga
     4321 cacgggcgaa aaacaggaag gcaccttcat gccgatggtt gccagcgatg gcccgcacta
     4381 tggcgcgaac atcaaaatga tgggcgtggg caactataaa gtgacctacc acattgaacc
     4441 gccgtcaaaa gccgggatgc atcgccatac tgacagcgaa acaggcgtag gccgctggtg
     4501 gaaaccgttt gatgtgagct acgagtttaa atatgtcggt ctgaactaaa accgactaac
     4561 caacgcgcca ggaggattcc cccttctggc gcgttggtca gaccgatcat atgcccgtcg
     4621 cttaattcaa cccgccgtac agagtcgaag ttatgagtta ctttttcgtc acgacgctac
     4681 aggtcttttt ctgcattgcg cttctttccg gtgttctctg gagtcgaaac gatcctccct
     4741 ctttacgtcc gctgacctgg acgctgctga ccgggctgat tgccggcgtg ctggcgggac
     4801 tgtttattca cggcagccaa ccggtacaac tgctgctggt tggggcggag gtcatggtat
     4861 cgctgctgtt tgtcctcagt ttctggtggg cttcaacgcg tatccgctat ctgtggcagg
     4921 ggattctgat tttcggcgct gcccgccatt gggcgctgga ccctaatctg ggcgggctga
     4981 ccagtacgca tgtgctgaat actgacctgc tgttaaacct gacggccgtc gttctcgctt
     5041 ttgcgatcct ctgtctggcg ggcgtactct gcgcgatgct gctgcggcgt attcgcggcc
     5101 tgtactggcc gttaacgctg attctgctgg tgatgatctg gctgccgctg agcggcaacc
     5161 tgctgttgtt gctgatgaaa ttacaggttg ttccgctggg aaaatcactg ctgagcttcg
     5221 tggcgaaagt cacaaataac acggcgctgt ataactgggc gggagccgcg ctgctgctgg
     5281 ccctggcgct gtgctggctt ccggcgctgt tgcgggcttt tcgacagacg cgggaaaccg
     5341 aagaaccgat tgctcaccgt ctggcgctgg cgcagcgtcg caacgctttg cggctgtggt
     5401 tggtcacaat cggctgcgcg gtggtcgtta tcgcaggcca actgtggtgg gataaagtgg
     5461 cctcgcagcc gccgcaactc tctgaagccg tgcccgtaac gctggggagc gatggcatgg
     5521 tgcgcctgcc ggttgaacag cttcgtgacg gtaaactgca tcgtttcgtc tgggtggcgg
     5581 acgatggcaa agcggtgcgc ttcttcgtga ttaaccgcta tcccgacaag ctgcgttttg
     5641 gcgtggtgtt cgacgcctgc ctgctgtgcg gcgatcaggg ttatgtcatg gagggcaatc
     5701 aggttatctg cgtcgcctgc ggagtgcata tttttattcc gtctatcggc aaggccggtg
     5761 gctgtaaccc ggtgccgatt gaaaactggc acaatgatga aaaagagctg gtgatccccg
     5821 gtaaagaact ggcgacaggg gtgaactact tctcgacggt catgactatt aaagtgaccg
     5881 atccggtcga tggttcaacg ttgaccaata cctcagccga ttacaaatac agctatggcg
     5941 gcaagacctg gttcttctcg tcagaggcta actatgaacg cttccgtgaa acgccggagc
     6001 agtttgttcc tgccgacatg agggaggaat aatcatgctg tggcggatgt tacgacaatc
     6061 ctgggggcgc aatttacgcc gcaaagtgct ggcgataatc accgtttttc tggcctccag
     6121 cctgatctcg gcgctgctgg cggtgtccat tgatatcggc gacaaaatgt ccagagagct
     6181 gaagtcttat ggtgcgaaca ttttgattga acccgccgga caggctgcgc tgcccgcgct
     6241 gtttagtgag agcagtaatc cgctttccgg tcaggatttt cttgatgaag ccgagctgcc
     6301 gaacatcaaa gatatcttct ggcgaaacaa cattgtcggg tttgcgccga tgctcggcgg
     6361 cgaagccagc gtggagggag aaccagtgcg gattctgggc actttcttca gtcagccagt
     6421 ggatattcct gatgaagagg gatatgagac ggggcagaaa acggtcagcc catactggca
     6481 ggtgaccggc gactggccgc aggaacccgc aggcgcagag ccgcagacct tagtcgggca
     6541 cgcgctggcg cggcaaatgg gctggaaacc cggtgataag ctgacattac gtaccgaagg
     6601 cgaagcagtg caggtcaccg tcagcggtat cctctccagc ggcggcgatg aagataacca
     6661 actggtgatg ccgctaagca cggtacagca cctgctgggg ctccccggaa aagtgcaggc
     6721 gattcgcgtc tcggcgctga cggtgccgga aaacgaactg tcccgccgcg cgcgggaaaa
     6781 cctggacgcg ctcaatgccg aagagtacga cctctggtat tgcacggcct atgtgtcgtc
     6841 gatcgcgcat cagctggaag aggcgatttc cggtgcggaa gtgcgcccgg tatggcaggt
     6901 cgcggcgtcc gaaggggtgg tgatcgataa aattcagttg ctgatggcgg tcgttaccgt
     6961 cgcggcgctg gtggcttccg caatggggat cgcctcgctg atgaccagca ccattatgga
     7021 acgcgccaaa gagatcggtt tgatgaaagc gctgggtgcg cgacagtggc aaattatgct
     7081 gctgttctac ctggaagcgg cgtccagcgg gctggctggc ggtgcgctgg gatgtattgc
     7141 cgggtggggg ctggcgaaag cgattggcgt catgctcttc gacgcgccgc ttaattttgc
     7201 ctggattgtg gtgccgtgcg tactggtgat tgcggtactg attgcgttga tcggcacctg
     7261 gttcccggcc cggcggattg cccggctgta tcctgtggag gtgctgtatg gccgctaaac
     7321 gtaccatgct ctggctgctg gtgtggcggg cgctgcgtct gcgttttcag cgggtcagcg
     7381 tggtgtttgc cgcgctgatg gtgggggcga ccatcgtgac ggcgcttttt gccgtctggt
     7441 tcgacattaa caccaaaatg agcgaagaac tgcgcacctt cggcgcgaat ttttacatcg
     7501 ggccggggca cggttccagt atgccgcagc aggagttgca gagcattctc gatcaggcgc
     7561 cgcaggggct ggtgcatggc gccagtccgt ggttgtacgg catggcgcgt accgagctgg
     7621 aaaaagtggt gatggttggc gtgtggttcg agtcgctgca aaaactggtg ccgtactggc
     7681 aggtcaccgg tagctggatt ggcgttagtt ttgacgaccg taatgcgatg atcggcgtca
     7741 aactggcgga acgcctcaac gtgcagccgg gcgacagcat cacgctggtg gatcacaatc
     7801 agcgtaagaa tttgcagatt aaaggcattg tggagtctgg cgatgcgacg gacaacatgc
     7861 tgatcgtcag cctcgacgtg gcgcaggcct ggctgcatca gccgggcaaa atcagccacg
     7921 gcttgctgag cgtcagtaac gatgtcggcc aggtggagaa ctatgccagc cggttgcagg
     7981 cgcagtatcc tgacctggag attcgtccgg tgcgtaaggt ctctgcttcc gaagggcagg
     8041 tgctggataa aattaaaggt ctgatgggac tggtctcgat cgtgattctg gcgctctctt
     8101 cgctgtgcgt gaataccacg ctgatggcga ttgtcggcga gcgggcgcgc gagtttgcgc
     8161 tgcaaaaagc gcttggttcc agcaacggcg atatcgtgcg gcaaattctg ctggaaacca
     8221 gcatcatcgc gctggcggcg gtggcgtgcg gctgggtgct gggatatttg ctggcgcaac
     8281 tgttgggatt aacggtgttt aatgccgcca tttcactgcg tctgccggtg ttgcccatta
     8341 ctctggtgtt gtctttactg gtcgccattc tggcggcgat tgttccggtg cgacgcgcgg
     8401 taagcgtcga gcctgctaaa gtgctgaaag gagagtagcg ccatgtccgt tacgcatatc
     8461 cctcaggtgg cgatcgaaac ccgccactta tacaaacggt tcggtgacgt taccgccctg
     8521 gatgacatta atctgcgcat tgcgcagggt gaatttgtcg ccattatggg cgcttctggt
     8581 tctggtaaaa ccacgctgat gaatattctg acgtgccttg atacggcgac ggaagggcag
     8641 gtgtttcttg atggcaccga tgccgccgcg ctggatgaag aggggcgccg tcgctttcgc
     8701 gccgagaaaa tcggcctggt atttcagcag ttccatttga tcccgttctt aactgcgctg
     8761 gaaaacatca tgctcgccca gcattaccac agcgtggtgg atgaagcggc ggcgaggaag
     8821 gtgctggaac aggtagggct tgggcatcgt gtgacgcacc tgccgagcca gctttccggt
     8881 ggtgagcagc agcgtgtgtg tatcgcccgg gcgctggtca acgaaccgcc ggttattttc
     8941 gcggatgaac cgacgggcaa cctggatgaa gagaatgagc agcgggtgct ggatctgctc
     9001 accgatctgc atcgtcaggg gcgcacgatt gtgatggtga cgcacaaccc tgcgcttggg
     9061 cagtttgccg accgtattct gcggctgcaa cacggtaaat atctgggtga ggaggccaat
     9121 caacatgcgc tggcataatg tgctgttact cgcggcggcg atcctgataa gcgggtgtaa
     9181 agaagaaaag ctggcggtcg gggcaccggc cccggcgctg gcggcgtttg atctgcaagg
     9241 ccaggagtca ggccttgaac gctggcaagg aaaggcggtg tacctgaatt tttggtctgc
     9301 gggatgcggg gggtgcctag cggaaatgga cacgctggaa gcgctgagca aaaaatgggg
     9361 cgataaagtg gtggttgtgg cggtcaatac cgaccctgaa acggtcagga tcaacgatct
     9421 gctggcaaaa catcagataa cctatccggt ggtgcgcgat cagctcaaca ttacccagga
     9481 acgttatcag gtgatcggca cgccgacctc cgtactgatt gactccgaag gacgcgttct
     9541 ggagctacac cagggtatgc gtaagccgtc tgagttagag gccacgtttg cgcgcctggc
     9601 ggcgcgatga acgacgcgta cggagagata cccaatggca tttatctgga acgacgaaag
     9661 ccttgcgatt ctgcgggaaa acgcgggcat cctcacgaca gagcagatag cgcaactgct
     9721 tcacactaat attaccgccg tgcggaatat ggcctatcgg ctgaaactga gccttcgggt
     9781 gacggcgtat aatcaccgac gcatagcgca ggttcaggcg ctttatgcct ctgagacgtt
     9841 gagtctcaaa gagatcgcgg cgaaaacggg gctgacggcc agtaccgtgc aatatatcgt
     9901 gtacgtgaaa tcaaaaaata agccctacgc caccacggaa tatgtctctt ttgaaacgga
     9961 gaacgccgtt cattaccgcg tgcaaaaaga atttgtcgat acggaacgtt ccctgctgga
    10021 caacatctct gataacacgc gatttcgtga actgtatctg acggacggca ctttttattg
    10081 cgcccgcaat attaagtatg aggtttttat ctcggagtaa ccggaacacg ctgacctgcc
    10141 ggttatcttc tggaatctcc cgttttgcga aaccgttccc ggaaaaatgt catatttctg
    10201 tcacactcta tgaggattga taacaacaag aggttgtagg gatgaaaaaa ttgatactcg
    10261 ccgccgctgt ggcaggggct gttttattgt cgtccgccgc gcaagcgcag acaacgccgg
    10321 aaggttatca actgcaacaa gtgttgatga tgagccgaca caacttgcgc gcgccgttgg
    10381 ccaataatgg cagcgtgctg gagcagtcca cgccgaatca gtggccggag tgggatgtgc
    10441 ccggcgggca gctcaccacg aaaggcggtg tgctggaaat ttatatgggc cactacatgc
    10501 gagaatggct ggcggagctg gggatggtca cgtcaggcga atgcccgacg ccggacacgg
    10561 tatataccta tgccaacagt ctgcaacgca ccgttgcgac ggcacaattc tttatcaccg
    10621 gcgctttccc tgggtgcgac attccggtac atcatcagga aaaaaatggg cacgatggac
    10681 ccaacgttta atccggtgat caccgatgac tctgcggcgt ttagccagaa agcggtgcag
    10741 gcgatggaaa aagagcgcag ccagatgcag cttgacgaca gctatcagct gctggcgcag
    10801 atgacggact acaaagattc accgtcctgc aaagaaaaac agcagtgttc gctgaccgaa
    10861 gccaaagacg cctttaggga aggtgcgaat aagcaggtca tgtcttccca agctgactcg
    10921 ttgattaaaa tttcgcggat ctgggccgat ttttgtcccg caaacacatc gaatcagcct
    10981 atttaggcta ttttttccac catttctgg
//
LOCUS       DAPOZO010000138        10633 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPOZO010000138 DAPOZO010000000
VERSION     DAPOZO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.138"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(95..790)
                     /gene="aqpZ"
                     /locus_tag="ROI21_004261"
     CDS             complement(95..790)
                     /gene="aqpZ"
                     /locus_tag="ROI21_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDX5113112.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGCFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRPLLEKRN"
     gene            1216..2874
                     /locus_tag="ROI21_004262"
     CDS             1216..2874
                     /locus_tag="ROI21_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDX5113113.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPELDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2871..3827)
                     /locus_tag="ROI21_004263"
     CDS             complement(2871..3827)
                     /locus_tag="ROI21_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDX5113114.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGERTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3978..5093
                     /gene="macA"
                     /locus_tag="ROI21_004264"
     CDS             3978..5093
                     /gene="macA"
                     /locus_tag="ROI21_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDX5113115.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5090..7036
                     /gene="macB"
                     /locus_tag="ROI21_004265"
     CDS             5090..7036
                     /gene="macB"
                     /locus_tag="ROI21_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDX5113116.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAVEKVNVAGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7109..7333)
                     /gene="cspD"
                     /locus_tag="ROI21_004266"
     CDS             complement(7109..7333)
                     /gene="cspD"
                     /locus_tag="ROI21_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDX5113117.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7656..7976
                     /gene="clpS"
                     /locus_tag="ROI21_004267"
     CDS             7656..7976
                     /gene="clpS"
                     /locus_tag="ROI21_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDX5113118.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8007..10283
                     /gene="clpA"
                     /locus_tag="ROI21_004268"
     CDS             8007..10283
                     /gene="clpA"
                     /locus_tag="ROI21_004268"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDX5113119.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT         2672 a         2510 c         2913 g         2538 t
ORIGIN      
        1 actatatgtg gcttaacaca tcaaggagcg caacaggaag atgaatcaat atatttaaaa
       61 aaggcctgac attgcgccag gccttctgcg ttaattaatt acgtttttcc agcaggggcc
      121 ggtaaatcag accaccgata ataccgccga caattggcac cacccagaag aaccacagtt
      181 gttctaacgc ccagccgccc tggaagatag caaccgcggt gctgcgcgcc gggttaacag
      241 aagtgttagt caccggaata ctgattaagt gaatcagggt taaggccaga ccaatagcga
      301 tcggcgcaaa accagcagga gcgaatttgt cggttgcgcc gtggatcacc agcaggaaac
      361 ctgcactcaa taccagttca actaccagcg cggaaagcat ggaataaccg cctggcgaat
      421 gctcgccata accgttagaa gcaaaaccgc tggctgccgc gtcaaaaccc gttttaccac
      481 tggcaattaa atacagcagc gccgccgcaa caataccgcc gacaacctgg gcaattacgt
      541 agccaacgac ttcttttgcc gggaaacatc cgccagccca taaaccaata gtgaccgccg
      601 ggttaaaatg accaccagaa atatgaccaa cagcaaaggc catcgtcaga acggtcagac
      661 cgaatgccag cgccacgccg gcaaaaccaa tgcctaattc cgggaagcct gcggccagta
      721 cggcactacc acagccacca aaaacaagcc agaaagtacc aaaacattca gctgctaatt
      781 ttctgaacat atccacctca attaaaaatt gaccctgtga aaaatatggt cgttttatag
      841 ggtcgtcgta aaaaagtgac gacggaaata atgcgcggct attttaaaaa cgaaggcaaa
      901 tcattcgcca gataaataaa tccagtaaat ttgatttagg gcaacagcgg gttgccccat
      961 atagtcattt gtctgattga cagtgtagtg caagcgaaag atttaatcct ttaggcgtaa
     1021 taaaaaataa tttatcatgc taattatttg attttgttgt ttttgcaggc ttatcagcaa
     1081 gagggagtat aacgcgatta ttcgctcatt tttcagacat ttgccatgct taaatgtgat
     1141 gtcatcacgt attagcaagg cctttcccgt tatactgcca gcgtaaagga taagtcacat
     1201 atttctggag gggatatgat tcttgagcgc gttgaaattg tgggttttcg cggtatcaac
     1261 cgtttgtcgt tgatgctgga acaaaacaac gtcctgattg gggagaacgc gtggggtaaa
     1321 tccagcttgc tggacgcctt aaccctgctg ctatcgccag aattagatct ctaccatttt
     1381 gagcgcgacg atttctggtt cccgccggga gatatcaacg ggcgagaaca tcatctgcat
     1441 attattttga ccttccgcga atcgctgcca ggccgacatc gggttcgccg ctatcggccg
     1501 ctggaagcgt gctggacgcc atgcaccgat ggctatcacc gtatttttta tcgtctggaa
     1561 ggggagagtg cggaagacgg cagcgtgatg acactgcgca gttttctcga taaagacgga
     1621 catccgattg atgtcgagga tattaacgat caggcacgcc atctggtgcg tttaatgccg
     1681 gtgctgcgct tgcgtgatgc ccgttttatg cgccgtattc gtaacggcac ggtgccaaat
     1741 gtccccaatg tggaagtcac cgcgcgccag ctcgatttcc tcgcccgtga gttgtcctca
     1801 catccgcaaa atctctctga tggacagatt cgtcagggac tttccgcaat ggtacagctg
     1861 cttgagcatt atttctctga gcagggggcc ggacaggcgc gatatcgttt aatgcggcgg
     1921 cgagccagca atgagcaacg aagctggcgc tatctggata tcatcaaccg gatgattgac
     1981 cgaccaggag ggcgctcgta tcgggttatt ttgctcggcc tgtttgctac tttgttgcag
     2041 gcaaaaggca cattgcgact ggataaagac gcccgtccat tgttgctgat cgaagatcca
     2101 gaaacccgtt tacaccccat tatgctttca gttgcctggc atctgttgaa tcttctgcca
     2161 ttgcagcgca ttgccaccac caactctggc gagctgcttt cgttaacgcc ggtagaacat
     2221 gtttgccgac tggtacgtga gtcctcgcgc gttgccgcct ggcgtctggg gccgagtggt
     2281 ttgagtaccg aagacagccg acgcatctct ttccacattc gttttaatcg tccgtcatcg
     2341 ctgtttgcac gctgctggct gctggtggaa ggcgaaacgg aaacctgggt tatcaatgaa
     2401 ctggcgcgtc agtgcggaca tcattttgat gccgaaggga tcaaggtcat tgagtttgcc
     2461 cagtccgggc taaagccact ggttaaattt gcccggcgaa tggggattga atggcatgta
     2521 ctggtcgatg gcgatgaggc agggaagaaa tatgccgcta cggtacgcag cctgttgaat
     2581 aacgatcggg aagccgaacg agaacattta acggcgttac cggcgctgga tatggaacat
     2641 tttatgtatc gccagggatt ttccgatgtg ttccaccgcg tggcgcaaat cccggaaaat
     2701 gtaccgatga atctgcgcaa aattatctcg aaagcgatcc atcgctcttc caaacccgat
     2761 cttgccattg aagtggcaat ggaggcagga cgtcgtggtg tggattccgt accgacgctg
     2821 ctgaaaaaaa tgttctcacg cgtgctgtgg ctggcgcgcg gtcgcgcgga ttaaccgcga
     2881 aacatcgtgg ccatttgtgg ctgaatagcg tcgagcatct catagcgccg acggtattca
     2941 gcccgttttt tactggcgat ttcggcaatc tcttttcgtg ctatctgtgc cggaaggcgg
     3001 taatggcgtt cagcatcaca tacgccgcct accgactccc agaacgcgtt gtaatcagca
     3061 tggatcttgc cttctttatc gcgataacgc aggctgcggt aaatatgtgt ttcattgctg
     3121 acggcaatga tctgctctac ctgcaaacgt tgggcaaaca gacaggccgc ttccatcaca
     3181 aggcgtttgg gaaatagccc gtggcaggct ttcgtcgcat tctggatttc ctgatgtgga
     3241 atttcccatt ttgcgccttg cagtccgcca ataaacattg ttctttcccc ctgatattca
     3301 cacagggtaa acgtgatctc tgccagagga ataccttcgc tgttgcggaa caggattgtg
     3361 ctgtcacctt ctttatccat tgagatcatc atggtcagct caagtgtgaa ctgctcgccg
     3421 tttttgcctt ccagcttcgc cagttgcagc ccgggggtat tcaaatataa gctgaattct
     3481 tccgccgaca tacatccgcg gagtaacgca taatggtaac gtaacgcctc cagcaattgc
     3541 ttacggctaa gattcgccgc aaggtaaggg cgatgcagac gcacaggcag tcgcggctgg
     3601 cgcgttaaca acacattgag attaggccag tgggaaagtt cgttcatcca ctcaacgctt
     3661 aaacgcggca taatcaacga gcgcagcaaa aatttctggc gaaaactacg gcgatgccag
     3721 aatttacccg gccgacactg tccacgtgcc agactaagaa aaagtgacag gctgctgaga
     3781 gattcagatg gcgtaaaggt ccgttcagtt agctgcgaca tattcatgaa atcaatggtt
     3841 atacatgacg tcgatttcac cattgcgtat cttaaccaaa catcaatagt gtgattacta
     3901 acgtaaattt tagggttttg ttgatatttc gttgaagtta atgacccgga ttggcatatg
     3961 gagtattcag aatatttatg aaaaagcgga aaaccgtgaa gaagcgttac gttattgcgc
     4021 tggtgatagt cattgccggg ctgattacgt tatggagaat tcttaacgca cccgtgccga
     4081 cttatcagac gctgattgtg cgccccggtg atttacagca aagcgtgctg gcgaccggaa
     4141 agctggacgc gctgcgtaag gttgatgtgg gcgcgcaggt cagcggtcag ttgaaaacgc
     4201 tgtcggtggc gattggcgat aaagtaaaaa aagaccagct tttaggggtt attgatcctg
     4261 aacaggctga aaaccagatt aaggaggtgg aagcaacgct gatggagcta cgcgcgcagc
     4321 ggcagcaggc ggaagcggag ctgaaactgg cgcgggtgac gtattcccgt cagcaacgtc
     4381 ttgcacaaac gcaagcggtt tcacagcagg atctcgatac cgccgcgacg gagatggctg
     4441 tgaaacaggc gcaaattggc accattgacg cgcaaatcaa gcgcaatcag gcctcactcg
     4501 atacggctaa aaccaatctc gattacaccc gcatcgttgc cccgatggcc ggggaagtca
     4561 cgcaaataac cactctgcaa ggccagacgg tgattgccgc acaacaagca ccgaacattc
     4621 tgacgctggc agatatgagc accatgctgg taaaagcgca ggtttctgaa gcggatgtaa
     4681 tccacctgaa gccggggcaa aaagcctggt ttacggtgct tggcgatcca ctgacgcgct
     4741 acgaggggca aatcaaggat gtgctaccga cgccggaaaa ggttaacgac gctattttct
     4801 attacgcccg ttttgaagtc cccaacccca atggtttgct gcggctggat atgaccgcgc
     4861 aagtgcatat tcagctcacc gatgtgaaaa atgtgctgac gatccctctg tcggcgttag
     4921 gcgatccggt tggcgataat cgttataaag tcaaattgtt gcgtaatggt gaaacacgcg
     4981 agcgtgaagt gacgattggc gcacgtaacg ataccgatgt tgagattgtc aaagggctgg
     5041 aagcgggcga tgaagtggtg attggtgagg ccaaaccagg agctgcacaa tgacgccctt
     5101 gctcgaatta aaggatattc gtcgcagcta tcctgccggt gatgagcagg ttgaggtgct
     5161 gaagggcatc accctcgata tttatgctgg tgagatggtg gcgattgtcg gcgcttcggg
     5221 ttccggtaaa tcgaccctga tgaatattct cggctgcctg gataaggcca ccagcggcac
     5281 ctatcgcgta gccggtcagg atgttgccac tctggacgcc gatgcgctgg cgcaactgcg
     5341 ccgcgagcat ttcggcttta ttttccagcg ttaccatttg ctttcgcatt taaccgccga
     5401 gcagaacgtt gaagtacccg ccgtctatgc tggtcttgag cggaaacagc ggctgcttcg
     5461 cgcccaggag ttgctgcaac gactgggact ggaagaccgt acagagtatt acccggcaca
     5521 gctttcgggg gggcagcaac agcgcgtcag catcgcgcgg gcattgatga acggcggtca
     5581 ggtaattctt gccgatgaac caaccggcgc actggacagc cattctggcg aagaggtgat
     5641 ggcgatcctg catcagctgc gcgatcgcgg gcatacggtg attatcgtta cccacgatcc
     5701 gcaggtcgct gctcaggccg agcgggtgat cgaaattcgc gacggcgaaa ttgtgcgcaa
     5761 tcctcccgcc gttgaaaaag tgaatgttgc tggcgggacg gaacctgttg tcaacacggc
     5821 ctctggctgg cggcaatttg tcagcggttt taacgaagcg ctgacgatgg catggagggc
     5881 gctggcagcg aataaaatgc gtactttact gaccatgctg gggattatta tcggtattgc
     5941 gtcggtggtt tccattgtcg tggtgggcga cgccgccaaa caaatggtgc tggcagatat
     6001 tcgttctatt ggtacgaata ctattgatgt ctatcccggg aaagattttg gcgatgacga
     6061 tccgcaatat cagcaggcgc tgaagtacga cgacttaatc gccatccaaa aacaaccgtg
     6121 ggtcgcctca gctacacctg ccgtctcgca aaacctgcgc ctgcgttata acaatgttga
     6181 tgttgctgcc agtgccaatg gcgtgagcgg cgattatttt aatgtctatg gcatgacctt
     6241 cagtgaagga aacaccttta atcaggagca gctgaacggt cgtgcgcagg tggtggttct
     6301 cgacagtaat actcgccgcc agcttttccc ccataaagca gatgtggttg gcgaggtgat
     6361 tctggtcggc aatatgcccg ccagagtcat tggtgtggcg gaagaaaaac agtcgatgtt
     6421 tggtagcagt aaagtgctgc gtgtctggct accttacagc acgatgtccg ggcgagttat
     6481 gggccagtcg tggctgaact ccattactgt cagggtgaaa gaaggatttg acagcgccga
     6541 ggcggaacag caactcacgc gtttactttc actgcgccac ggtaagaagg atttctttac
     6601 ctggaacatg gacggcgtct tgaaaaccgt tgaaaagacc acacgtactt tacaactgtt
     6661 tctgacgctg gtggcagtga tttcgctagt ggtgggcggt attggtgtaa tgaatattat
     6721 gctggtgtca gtgaccgagc ggacgcggga aattggcatt cgcatggctg taggtgcgcg
     6781 agcaagtgat gtgttgcaac agttcctgat cgaagccgtg ctggtttgcc tggtcggtgg
     6841 cgcgttggga ataacactgt cactgttaat tgctttcacc ttgcagcttt tcttacccgg
     6901 ctgggagatt ggtttttcac cgttggcgct gctgctggcg tttctctgct cgacggtcac
     6961 cgggatttta tttggctggt tacccgcacg aaatgcggca cgactggatc cagtagatgc
     7021 tctggcacga gagtaatttt tgagataaaa atgccagccg atcgggctgg cattttgcct
     7081 ttaggatgta cacaatgaga cagaagagct atgcgactgc cgcttctact tcgacgggca
     7141 caataacact ggcgtgattg ccttttggcc cctggtggac atcaaactga acggattgtc
     7201 cagcttttag cgttctgtaa ccatccatct gaatggtgga ataatgagcg aaaatatctt
     7261 cgccgccgcc ttcagggcag atgaaaccaa accctttggc attgttgaac cacttaacag
     7321 tacccttttc catgcttcga catccttcgc aaatcttata caagtaagat ggaataaacc
     7381 ggggtcagag agggggctgt tcaaaacctc gccaactcta gaaatacaat ttagagaatt
     7441 agggcgagcc gtcaagcatt tgacaggggc cagggggcag gtatgaatca aaaatttgaa
     7501 gcagttaacg ctattgacag gaatgtgaca gatgtcgctg atgccaacga tagatgatag
     7561 ttatctatca tgtggagtag attggtcagg caaataagct cttgtcagcg gcagggtgtt
     7621 ctgccgataa ccgtaaccga agatgataac tgacaatggg taaaacgaac gactggctgg
     7681 actttgatca actggcggaa gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata
     7741 aagtgatatt agtcaatgat gattacactc cgatggagtt tgttattgac gtgttacaaa
     7801 aattcttttc ttatgatgta gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg
     7861 ggaaggccat ttgcggagtc tttaccgccg aggttgcaga aaccaaagtg gcgatggtga
     7921 acaagtacgc gagggagaat gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc
     7981 aggcataaaa attgggggag gtgcctatgc tcaatcaaga actggaactc agtttaaata
     8041 tggctttcgc cagagcgcgc gagcaccgtc atgagtttat gaccgtcgag cacttgttac
     8101 tggcgctgct cagtaaccca tctgcccggg aggcgctgga agcgtgttct gtggatttgg
     8161 ttgcgctccg tcaggaactg gaagccttta ttgaacaaac cacacccgtt ctgcctgcca
     8221 gtgaagagga gcgcgacaca cagccaacgc tgagttttca gcgtgtactg caacgtgcgg
     8281 tcttccatgt ccagtcctcc ggtcgcaatg aggttaccgg tgcaaacgtt ctggtcgcta
     8341 tctttagcga acaggagtcg caggcggcat atctgttgcg taaacatgaa gtcagccgtc
     8401 tcgatgtggt gaactttatc tctcatggca cgcgtaaaga cgagccgaca cagtcttctg
     8461 atcctggcag ccagccaaac agcgaagaac aagctggtgg ggaggaacgt atggagaatt
     8521 tcacgacgaa cctgaatcag cttgcgcgcg tgggcggaat cgacccactg attggtcgtg
     8581 agaaggagct ggagcgtgct attcaggttc tctgccgtcg ccgtaaaaac aacccgctgc
     8641 tggtggggga atctggtgtc ggtaaaaccg cgattgcaga aggtcttgcc tggcgaattg
     8701 ttcagggcga tgtgccggaa gtgatggctg actgtacgat ttactctctc gatatcggtt
     8761 ctctgttagc gggcacaaaa tatcgcggcg actttgaaaa acgttttaaa gcgttgctca
     8821 agcagctgga gcaggacact aacagcatcc tgtttattga tgagatccac accattatcg
     8881 gtgcgggtgc agcgtctggt ggtcaggtcg atgcggctaa cctaatcaaa ccgttgctct
     8941 ccagcggtaa aattcgcgta attggttcga caacctatca ggagttcagc aacattttcg
     9001 agaaagaccg tgctctggcg cgtcgcttcc agaaaattga tattactgaa ccgtcgatcg
     9061 aagaaactgt tcaaatcatc aatggcctga aaccgaagta tgaagcgcac cacgacgtgc
     9121 gttataccgc aaaagcggtg cgtgcagcgg tagagctggc ggtgaaatac attaacgatc
     9181 gtcatctgcc ggataaagcc attgacgtta tcgacgaagc gggcgctcgc gcacgcctga
     9241 tgccggtaag caaacgcaag aaaaccgtta atgtggcgga tattgagtcc gtggtggccc
     9301 gtattgcgcg cattccagag aagagtgttt ctcagagtga ccgcgatacc ctgaaaaacc
     9361 tcggcgatcg cctgaaaatg ctggtcttcg gtcaggataa agccatcgag gcgctgactg
     9421 aagccattaa gatggcgcgt gcaggtttag gtcacgaaca taaaccggtc ggttcgttcc
     9481 tgtttgccgg ccctaccggg gtcgggaaaa cagaggtgac ggtacagctt tcgaaagctt
     9541 tgggcattga gcttctgcgc tttgatatgt ccgagtatat ggaacgccat accgtcagcc
     9601 gtctgattgg tgcgcctccg ggatacgttg gttttgatca gggcggtttg ctgactgatg
     9661 cggtcatcaa gcatccacat gcggtgctgc tgctggacga aatcgagaaa gcgcacccgg
     9721 acgtgttcaa tattctgttg caggtgatgg acaacggtac gctgaccgat aacaacggac
     9781 gcaaagcgga cttccgtaac gtggtattgg tgatgaccac caacgctggg gtacgagaaa
     9841 ctgagcgtaa atcgattggt cttatccacc aggacaacag caccgatgcg atggaagaga
     9901 tcaagaagat ctttacgccg gagtttcgta accgtctcga caacattatc tggttcgatc
     9961 atctgtcaac cgacgtgatc catcaggtag tggataaatt catcgtcgag ttgcaggttc
    10021 agttggatca gaaaggtgtt tctctggaag tgagccagga agcgcgtaac tggctagccg
    10081 agaaaggtta cgaccgggca atgggcgcac gtccgatggc gcgtgtcatc caggacaacc
    10141 tgaaaaaacc gctcgccaac gaactgttgt ttggttcgct ggtggacggc ggtcaggtga
    10201 cggttgcgct ggataaagag aaaaatgagc tgacttatgg attccagagt gcacaaaagc
    10261 acaaggcgga agcagcgcat taatctaatt gttaggtagg ttggtgaagt ccgtaatctc
    10321 gaaagaggtt acggactttt tatttatggg gtggaggagg ttcagaccct ttttttgatg
    10381 atgatggtaa gttgttgata attagtgctg cgggaaggta aggataaaaa agggtgctgc
    10441 aggagaatgg gatggttttg ctttattaac aacgggctaa acgtgtagta tttgagttca
    10501 ttgccgtaca ggcagcttag aaagccaacc gaaagaaaaa ctcgattttt ataccgttca
    10561 ctgccgtaca ggcagataaa atgcgaaaaa aaactcgtac tttcgtacga gctcttcttt
    10621 gaatatggcg gtg
//
LOCUS       DAPOZO010000139        10580 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPOZO010000139 DAPOZO010000000
VERSION     DAPOZO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.139"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(21..1688)
                     /gene="ettA"
                     /locus_tag="ROI21_004269"
     CDS             complement(21..1688)
                     /gene="ettA"
                     /locus_tag="ROI21_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDX5113120.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            1899..3836
                     /gene="sltY"
                     /locus_tag="ROI21_004270"
     CDS             1899..3836
                     /gene="sltY"
                     /locus_tag="ROI21_004270"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDX5113121.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPVVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALIQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            3926..4252
                     /gene="trpR"
                     /locus_tag="ROI21_004271"
     CDS             3926..4252
                     /gene="trpR"
                     /locus_tag="ROI21_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDX5113122.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(4246..4761)
                     /gene="yjjX"
                     /locus_tag="ROI21_004272"
     CDS             complement(4246..4761)
                     /gene="yjjX"
                     /locus_tag="ROI21_004272"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDX5113123.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            4813..5460
                     /gene="gpmB"
                     /locus_tag="ROI21_004273"
     CDS             4813..5460
                     /gene="gpmB"
                     /locus_tag="ROI21_004273"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDX5113124.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWGVETAGDISHLDAPALDELQR"
     gene            complement(5457..6326)
                     /gene="robA"
                     /locus_tag="ROI21_004274"
     CDS             complement(5457..6326)
                     /gene="robA"
                     /locus_tag="ROI21_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDX5113125.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            6537..7010
                     /gene="creA"
                     /locus_tag="ROI21_004275"
     CDS             6537..7010
                     /gene="creA"
                     /locus_tag="ROI21_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDX5113126.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            7023..7712
                     /gene="creB"
                     /locus_tag="ROI21_004276"
     CDS             7023..7712
                     /gene="creB"
                     /locus_tag="ROI21_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDX5113127.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            7712..9136
                     /gene="creC"
                     /locus_tag="ROI21_004277"
     CDS             7712..9136
                     /gene="creC"
                     /locus_tag="ROI21_004277"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDX5113128.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYIYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKKITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVEQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            9194..10546
                     /gene="creD"
                     /locus_tag="ROI21_004278"
     CDS             9194..10546
                     /gene="creD"
                     /locus_tag="ROI21_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDX5113129.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLDMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
BASE COUNT         2465 a         2669 c         2943 g         2503 t
ORIGIN      
        1 cgcatccggc attttacgca ttacttcgca atacgcttgt acttgatacg cttcggctcc
       61 agcgcgtctg cgcccagcgt gcgtttcttg tactcttcgt actcggtaaa gttaccttcg
      121 aagaactcaa ctttaccttc atcctggtaa tccaggatgt gcgtggcgat acggtcgagg
      181 aaccaacggt cgtgcgagat aaccatcgca cagcccggga actccagcag ggcgttttcc
      241 agcgcgcgca gggtttcgat atccaggtcg ttggttggtt cgtcgagcag cagcatgttg
      301 ccgccaactt gcagcagctt cgccagatgc agacgaccgc gctcaccgcc ggagagttca
      361 ccaacgcgtt taccctgatc aaccccttta aagttaaagc ggccaacgta ggcgcggctt
      421 ggcatctcgg tgttgccgat tttcatgata tccagcccgc cggaaacttc ttcccaaacg
      481 gttttgctgt tatccattga atcacggaac tgatcaaccg atgccagttt caccgtttca
      541 cccaaagtga tggtgccgct gtccggctgt tcctgaccgg agatcatacg gaacagggtc
      601 gatttacccg caccgttcgg accgatgatc ccgacgatcg cccctttcgg gatcgagaaa
      661 ctcagatcat caatcagcag acgatcgcca taggatttac gcagattgct gacttccagc
      721 actttgtcgc ccagacgcgg tccaggtgga ataaacagtt cgttggtttc gttacgtttc
      781 tgatattcgg tgctgttcag ctcttcaaag cgtgccagac gtgctttacc tttcgactga
      841 cggcctttag tcccctgacg tacccactcc agctctttct cgatcgactt acgacgcgcc
      901 gcttcttgtg aagcttcctg cgccaggcgc tgatctttct gctccagcca agaggagtag
      961 ttaccttccc acggaatacc ttcaccgcgg tccagttcga ggatccagcc cgccacgtta
     1021 tcgaggaagt aacggtcgtg ggtaatcgcc acaacggtgc cttcgaagtc gtgcaggaag
     1081 cgttccagcc aggccacgga ttcggcatcc aggtggttgg tcggttcgtc gagcagcagc
     1141 atgtctggtt tttccagcag caggcggcac aacgctacgc gacgacgctc accaccggag
     1201 aggttagcga ttttcgcgtc ccagtccggc agacgcagcg catccgccgc acgctccagc
     1261 tgaacgttca ggttgtgacc gtcgtgagcc tgaatgatct cttccagacg gccttgttca
     1321 gcggccagct tgtcaaaatc ggcatccgga tcggcgtaca gcgcatacac ttcatccagg
     1381 cgtttcaggg cgttaaccac ttcagaaacc gcttcttcaa tggactcacg cacggtgtgt
     1441 tccgggttca gctgcggttc ctgcggcaga taaccaatct tgatatccgg ttgcggacgc
     1501 gcttcgcctt cgatgtcttt atcaatgccc gccataatgc gcagcagggt ggacttaccc
     1561 gcgccgttca gacccaggac accaattttt gccccaggga agaaactcag agagatgttt
     1621 ttcaaaatat gacgtttcgg cggaacaact ttgccgacac gatgcatggt ataaacgaat
     1681 tgagccacgt tggacttcgc ctctatgttt atcgtgatga tgagttttca aaggcgaagt
     1741 gtagcctttt tccccgccta atcccagccg gtcgatcacc ctcgcagtaa aagtaaaaaa
     1801 gtgtccgtaa cgtggcgtaa acggcaatga ctggttagca taaatctatt acgcggcatg
     1861 acgctgcatt gatgtattta cacttagagg atgcgcttgt ggaaaaagcc aaacaagtta
     1921 cctggcggct gttggctgcc ggtgtctgtc tgctgacggt cagcagcgtg gcgcgagccg
     1981 actcactgga tgagcagcgt agccgctacg cccaaatcaa gcaggcctgg gataatcgac
     2041 aaatggatgt ggtcgaacaa atgatgcctg gactgaagga ttatccgctt tatccctacc
     2101 tggaataccg tcagatcacc gatgacctga tgaatcaacc ggtggtgacg gtcactaact
     2161 ttgttcgcgc taaccccacg cttcctcccg ctcgcacgct gcaatctcgt ttcgtcaatg
     2221 aactggcgcg gcgtgaagac tggcgtggct tgttagcctt tagcccggaa aagcccggaa
     2281 ctaccgaagc gcaatgtaat tactactatg cgaaatggaa caccgggcag agtgaagaag
     2341 cctggcaagg ggcgaaagag ctgtggctaa ccggcaagag ccagcctaac gcctgtgaca
     2401 agttatttag cgtctggcgt gcgtcaggta aacaagatcc gctggcgtat ttagagcgta
     2461 tccgtctggc gatgaaagcg ggtaacactg gcctggtaac agtgctggca gggcagatgc
     2521 ctgccgatta ccagactatc gcttcggcaa tcatttcact ggcgaacaac cctaatacgg
     2581 tactgacctt cgcgcgtaca accggagcga ccgattttac ccgtcaaatg gcggcggtgg
     2641 cgtttgccag tgtggcgcgg caggatgctg aaaatgcacg gctgatgatc ccgtcgcttg
     2701 cccaggcgca gcagcttaat gaagatcaga ttcaggagct gcgcgatatc gtcgcctggc
     2761 gtttgatggg caatgatgtc accgatgagc aggcgaaatg gcgcgatgac gccattatgc
     2821 gttcgcaatc tacttcgctt attgaacggc gcgtgcgaat ggcgcttggc accggcgatc
     2881 gtcgcggcct gaatacctgg ctggcgcgtt tgccgatgga ggcgaaagag aaagatgaat
     2941 ggcgttactg gcaggcggat ttactgctgg aacgcggacg tgaagctgaa gcaaaagaga
     3001 ttttgcatca actcatgcaa cagcgtggtt tctacccgat ggttgcagca caacgcatcg
     3061 gcgaagagta tgagctgaag attgataaag cgccgcagaa tgttgacagc gccctgattc
     3121 aggggccgga gatggcgcgc gtgcgcgagt tgatgtactg gaatctcgat aacaccgcgc
     3181 gtagcgagtg ggccaatctg gtgaagagca agtctaaaac agagcaagct caactggcgc
     3241 ggtatgcttt caacaaccaa tggtgggatc ttagcgttca ggcaacgatc gccgggaagc
     3301 tgtgggatca tctggaagaa cgattcccgc tggcctacaa cgatcttttc aaacgttaca
     3361 ccagtgggaa ggagatcccg caaagctatg cgatggcgat tgcccgtcag gagagtgcct
     3421 ggaatccgaa agtgaaatcg ccggtagggg ccagcggcct gatgcagatt atgccgggca
     3481 cggcgaccca tacggtgaag atgttctcta ttcctggtta tagcagccca ggacaattgc
     3541 tggatccgga aacgaacatc aacattggca ccagttacct gcaatatgtt tatcagcagt
     3601 ttggtaataa ccgtattttc tcctcagcag cttataacgc cggaccaggg cgggtgcgaa
     3661 cctggcttgg caacagcgcc gggcgtatcg acgcagtggc atttgtcgag agtattccgt
     3721 tctccgagac gcgcggttat gtgaagaacg tgctggctta tgacgcttac taccgctatt
     3781 tcatggggga taaaccgaca ttgatgagcg ccacggaatg gggacgtcgt tactgatccg
     3841 cacgtttatg atatgctatc gtactcttta gcgagtacaa ccgggggagg cattttgctt
     3901 cccccgctaa caatggcgac atattatggc ccaacaatca ccctattcag cagcgatggc
     3961 agaacagcgt caccaggagt ggttacgttt tgtcgacctg cttaagaatg cctaccagaa
     4021 cgatctccat ttaccgttgt taaacctgat gctgacgcca gatgagcgcg aagcgttggg
     4081 gactcgcgtg cgtattgtcg aagagctgtt gcgcggcgaa atgagccagc gtgagttaaa
     4141 aaatgaactc ggcgcgggca tcgcgacgat tacgcgtgga tctaacagcc tgaaagccgc
     4201 gcccgttgag ctgcgccagt ggctggaaga ggtgttgctg aaagatcact gatacaccgc
     4261 attatgaaac ggactcagcg ccaggatcac cgcctggtga tagacgctgg cgcgagtgag
     4321 tttcccggcg gtaaacacgc cgatcgcccc ttccttacga ccaatctcat caataccggt
     4381 atagcgcgac atcacgggac cgagcgcctc gccttcacgc actttttcca gaatcaccgc
     4441 aggtaacggc aaagtagccg aacgcgcttc gccgcgctgg ctggcgtttt caatcaccac
     4501 ccaactgaag gtgctgtcgc catcaatgcc agcttcaatc gccacccaaa aatcagcctc
     4561 tggatgtaaa cggcgggcat ttgctacccg atttcgtgcg ccagcgcgcg tttcctcact
     4621 gccaaagggc tgttccggta caccgctctc gacggcaacg gatgcaatat ggcaggatcc
     4681 ttcgccgaag atctcgtgaa atgcctgcag aatggcctga attttagcgg gattggtggt
     4741 cgcacagaca acttggtgca taatcagcat tactcagaaa attaacgtta cagcagtata
     4801 cggaaaaaaa gcatgttaca ggtataccta gtccgccacg gtgaaacgca gtggaacgcc
     4861 gagcgacgta ttcagggcca gtctgacagc ccgctgaccg ccaaaggtga gcaacaggcg
     4921 atgcaggtgg caacccgtgc caaagagctt ggcattacgc atatcatcag tagcgattta
     4981 ggacgcaccc ggcgtacggc ggaaatcatc gcccaggcct gcggctgtga catcatcttt
     5041 gattctcgcc tgcgtgaatt aaacatgggt gtgctggaaa aaagacatat cgattctctg
     5101 accgaagaag aagagaactg gcgtcggcag ctggtcaatg gcaccgttga cgggcgtatt
     5161 cctgaaggcg agtcaatgca ggagctcagc gatcgcgtca atgcggcact ggaatcttgc
     5221 cgggacttac cgcagggaag ccgaccgctg ctggtgagcc acggtattgc actgggatgc
     5281 ctggtgagta cgattctcgg attaccagca tgggcagagc gtcgcttacg tctgcgtaac
     5341 tgttctattt cgcgcgtgga ttatcaggaa agcctgtggc tggcgtctgg ttggggcgtt
     5401 gaaaccgcag gggacatctc gcatctggac gcccctgcat tagatgagct gcagcgttaa
     5461 cgacggatcg gaatcagcag ttcacagcgc agattaattg ggcgatctcc cgctttggca
     5521 tcttctgccg ggtagtatcg ctcaatatcc tgacctttac ggcgcgtcag gttgagcatt
     5581 ggcatacacg ttccgtatac cgtcaggata aactcctgca cgccggttcc cagaccttca
     5641 taggtaaaca tcacatattc gccgccctgc agcatcaccg gatgccccgt cagtacatag
     5701 ccatctgcct gatcctgggc taacgcggtg gtatagaata cttcttgttc gtcgtcttta
     5761 tcctgactcg gacgcgtttc attcaggccg tagagcaccg gcggaatggt cggcgcgttg
     5821 ccgagaaaat cgtgccagaa ctgataacgc atttcatggc ggaaatcaga gatttgctcc
     5881 agcgaacagg agtagctctg ggtaacacca atcagcggcg tatcttccag ggtgacaaat
     5941 ttgtgctctg gcatagtgaa ttcacccaga cgtagcggcg ggcgaatacc aaaggcgctc
     6001 cattcaggag aacggcggta aagtgcagga gtctgggcaa actgcttctt gaatgcgcgg
     6061 gtaaatgtct gttgagagtc gaagcggtat tgcagcgcga tgtccagaat cggacgcgca
     6121 gtcaggcgta gtgcgaccgc cgatttcgac aaacgacgag cacgaatata cgcgccaata
     6181 gcatggccag tgacatcttt aaacattctc tgtaagtgcc acttggaata acctgctttc
     6241 gccgctacat tgtcgagcga caggggctga tccagatgac cttccagcca gattaaaagg
     6301 tcgcgaataa tgccggcctg atccataaaa tatcctcatc ctttcaacaa cgagcacctg
     6361 acatcaggta attggataat agcatttttt gctgttttag cattcagtgt ttttttctta
     6421 gtagagtatg ttttaggact tgacggaaat aaaagtattg agattttgtt cttaatcaat
     6481 atgttattta ccgtgacgaa ctaattgctc gtgtaataga taaaaatggt aacaatatga
     6541 aatacaagca tttgatcctg tctttaagcc tgataatgct ggggccattg gctcatgcag
     6601 aagagattgg ttcggtcgac accgtattta aaatgatcgg cccggatcac aaaattgttg
     6661 tggaagcctt tgacgatccc gatgtgaaaa atgtcacctg ttatgtgagc cgggcgaaaa
     6721 ccggtggtat taaaggggga ttgggcctgg cagaagatac ctccgatgcg gccatttctt
     6781 gtcagcaagt cgggccgatt gaactgtcgg atcgtattaa aaacggcaaa gctcagggcg
     6841 aggtagtatt caaaaaacgc acgtccctgg tctttaagtc gttacaggtc gtgcgctttt
     6901 atgatgccaa acgcaacgcg ctcgcttatc tggcttactc cgacaaagtt gtagaaggtt
     6961 cgccgaaaaa cgcgattagc gcggttcctg tcatgccgtg gcggcaataa cagaggcgat
     7021 ttatgcaacg ggaaacggtc tggttagtgg aagatgagca agggatagcc gacacgctgg
     7081 tctacatgtt gcagcaggaa ggttttgccg tcgaggtctt tgagcgaggc ttgccggtgc
     7141 tggataaagc tcgccagcag gtacccgacg tcatgattct cgatgttggt ctgccggata
     7201 ttagcggctt tgaattgtgc cgccagttac tggcgctcca tccggcgtta cctgtactgt
     7261 tcctgacggc ccgaagtgaa gaggtcgatc gcctgcttgg gctggaaatt ggcgctgacg
     7321 actatgtggc taaaccgttt tcaccccgcg aagtgtgcgc cagggtgcgc accttactgc
     7381 gtcgggtgaa gaagttctcg acgccgtctc ccgtcatccg tattggacat tttgaattga
     7441 atgaacccgc ggcgcagatc agctggtttg acacgccatt aacgctgacg cggtatgagt
     7501 ttttattgct gaagacgtta ctcaagtcgc caggccgcgt ctggtcccgc cagcaactga
     7561 tggatagcgt gtgggaagat gcgcaggaca catacgatcg caccgtcgat acccacatta
     7621 aaacgttgcg tgccaagctg cgcgccatca accccgatct ttcaccgatt aatactcatc
     7681 gcggcatggg atatagcctg aggggcctgt aatgcgtatc ggcatgcggt tgctgctggg
     7741 ctatttttta ctggtggcgg tggcagcctg gttcgtactg gccatttttg tcaaagaagt
     7801 taaaccgggc gtgcgaagag caacggaggg gacgttgatc gacaccgcaa cgttgctggc
     7861 agagctggcg cgtcccgatt tgctctctgg ggacccaacg catgggcaac tggcgcaggc
     7921 gtttaatcag ctacagcatc gcccgtttcg cgccaatatc ggtggcatta acaaagtgcg
     7981 caatgaatat catgtctata tgaccgatgc gcagggcaaa gtattgttcg attcggcaaa
     8041 taaagccgtt ggacaggatt attcgcgctg gaatgacgtc tggctaacgt tgcgtggtca
     8101 gtatggtgcg cgcagcacgt tgcaaaatcc tgccgatccc gaaagttctg tgatgtatgt
     8161 tgccgcgccg attatggacg gctcgcggct tattggcgtt ttgagcgtag gcaaaccgaa
     8221 cgcggcgatg gctccggtca ttaagcgcag cgagcggcga attttatggg ccagcgccat
     8281 tttgttgggg attgcactgg tgattggcgc aggcatggtt tggtggatca accgctccat
     8341 tgcccggctc actcgctatg ctgattccgt cactgacaat aagcccgttc ctcttcctga
     8401 actcggcagt agcgaactgc gtaagcttgc gcaggcgctg gaaagtatgc gcgtgaagct
     8461 ggaggggaaa aactatattg agcaatatat ttatgcgtta actcatgagc taaaaagccc
     8521 actggcggcg attcgtggcg cggcggaaat tttacgcgaa ggtccgccgc ctgaagtagt
     8581 ggctcgtttt actgacaaca ttctgacaca aaatgcgcgt atgcaggcat tggtagaaac
     8641 gttactacgc caggcaagac tggagaatcg tcaggaagtc gttctgactg ctgttgatgt
     8701 ggcggcatta ttccgccgcg tcagcgaagc gcgcaccgtg cagttggcag aaaaaaaaat
     8761 cactctgcat gttacgccca ccgaggttaa cgttgctgct gaaccggcgt tactggagca
     8821 ggctctgggg aatttactgg ataacgccat cgattttacc cccgagagcg gttgcatcac
     8881 gctaagcgcc gaagtggagc aggaacacgt cacgcttaag gtgctggata ccggtagtgg
     8941 tattcctgac tacgcgcttt cacgtatttt tgaacgcttt tactctttgc ctcgtgcaaa
     9001 tgggcaaaaa agcagcggtc tggggttggc gttcgtcagt gaggtcgccc gtttgtttaa
     9061 cggcgaagtc acgctgcgca acgtgcagga aggtggcgtg ctggcttcgc ttcgacttca
     9121 ccgtcacttc acatagcttc aaattcttcc cacatagcct tcgtatcctg ctgccattgc
     9181 aaaggagaag actatgttga aatcccccct gttctggaaa atgaccaccc tgtttggtgc
     9241 agtattgctg ttgttgattc cgataatgct gattcggcag gtgattgtcg aacgtgctga
     9301 ttaccgtagc gatgtggaag atgcgattcg ccaaagtacc agcgggccgc aaaaactcgt
     9361 tgggccgctc atcgctattc ctgtgaccga gctttatacg gtgcaggaag aggataaaac
     9421 cgtggagcgg aaacgaagtt ttatccattt ttggttacct gagtcattga tggttgatgg
     9481 caatcagaac gtggaagaac gcaagatagg gatttatacc ggtcaggtct ggcacagtga
     9541 tttaacgcta aaagccgatt tcgatgtttc gcgtcttagc gaactcaacg cgccaaatat
     9601 caccttaggc aagccattta ttgtgattag cgtcggggat gcgcgtggta ttggtgtggt
     9661 gaaagcgcct gaagttaacg gaacggcgct gaccattgaa cccggcaccg ggttagagca
     9721 aggcgggcag ggcgtgcata tccctttacc cgaaggggac tggcgcaagc agaacctgaa
     9781 gctggatatg gcactgaatt taagcggtac cggcgatctt tctgtggtgc ctgccgggcg
     9841 taatagcgaa atgaccttaa ccagcaactg gccgcatccc agttttttag gtgattttct
     9901 accagccaaa cgggaagtta gcgagtcagg ttttcaggcg caatggcaaa gcagctggtt
     9961 tgctaataat ctcggtgagc gttttgcttc aggcaatgat accggctggg aaaacttccc
    10021 cgcgtttagc gtcgcagtga cgacgccagc cgatcaatac caattaacgg accgggcgac
    10081 taagtacgcc attctgctga ttgcactgac ttttatggcg ttctttgttt ttgaaacgct
    10141 caccgcgcaa cgtttacacc caatgcaata tttgctggtg gggctttcat tggtgatgtt
    10201 ttatttgctc ttgctggcgc tttctgaaca tatcggtttt accgtggcat ggataatagc
    10261 cagtctgatt ggggcgttaa tgaacggtat ttatttgcag gcggtattga aaggctggcg
    10321 caatagcatg ttgtttaccc tcgcgctgtt gttgctggat ggtgtgatgt ggggactgct
    10381 caactctgcc gatagcgcgc tgttgttggg aaccagtgtg ctggtggtgg cgctggccgg
    10441 catgatgttt gtgacccgta atatcgactg gtatgcgttt tcactgccga aaatgaaagc
    10501 cagtaaagaa gttacaacgg acgatgaatt acgtatctgg aaataaggtt gaaaaataaa
    10561 aacggcgcta aaaagcgccg
//
LOCUS       DAPOZO010000140        10300 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPOZO010000140 DAPOZO010000000
VERSION     DAPOZO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.140"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(227..303)
                     /locus_tag="ROI21_004279"
     tRNA            complement(227..303)
                     /locus_tag="ROI21_004279"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(267..269),aa:Arg,seq:acg)
     gene            complement(307..399)
                     /locus_tag="ROI21_004280"
     tRNA            complement(307..399)
                     /locus_tag="ROI21_004280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(363..365),aa:Ser,seq:gct)
     gene            complement(715..900)
                     /gene="csrA"
                     /locus_tag="ROI21_004281"
     CDS             complement(715..900)
                     /gene="csrA"
                     /locus_tag="ROI21_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDX5113130.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1135..3765)
                     /gene="alaS"
                     /locus_tag="ROI21_004282"
     CDS             complement(1135..3765)
                     /gene="alaS"
                     /locus_tag="ROI21_004282"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDX5113131.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3894..4394)
                     /gene="recX"
                     /locus_tag="ROI21_004283"
     CDS             complement(3894..4394)
                     /gene="recX"
                     /locus_tag="ROI21_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDX5113132.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4463..5524)
                     /gene="recA"
                     /locus_tag="ROI21_004284"
     CDS             complement(4463..5524)
                     /gene="recA"
                     /locus_tag="ROI21_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDX5113133.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5604..6101)
                     /gene="pncC"
                     /locus_tag="ROI21_004285"
     CDS             complement(5604..6101)
                     /gene="pncC"
                     /locus_tag="ROI21_004285"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDX5113134.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6246..7331)
                     /gene="mltB"
                     /locus_tag="ROI21_004286"
     CDS             complement(6246..7331)
                     /gene="mltB"
                     /locus_tag="ROI21_004286"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDX5113135.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLTAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7587..8150
                     /gene="srlA"
                     /locus_tag="ROI21_004287"
                     /gene_synonym="gutA"
     CDS             7587..8150
                     /gene="srlA"
                     /locus_tag="ROI21_004287"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDX5113136.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8147..9106
                     /gene="srlE"
                     /locus_tag="ROI21_004288"
                     /gene_synonym="gutE"
     CDS             8147..9106
                     /gene="srlE"
                     /locus_tag="ROI21_004288"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDX5113137.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLEQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9117..9488
                     /gene="srlB"
                     /locus_tag="ROI21_004289"
                     /gene_synonym="gutB"
     CDS             9117..9488
                     /gene="srlB"
                     /locus_tag="ROI21_004289"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDX5113138.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9492..10271
                     /gene="srlD"
                     /locus_tag="ROI21_004290"
     CDS             9492..10271
                     /gene="srlD"
                     /locus_tag="ROI21_004290"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDX5113139.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
BASE COUNT         2356 a         2821 c         2551 g         2572 t
ORIGIN      
        1 gtactctatc cagctgagct acggatgcat cgggaaactt actttactgc agattttttg
       61 ataccgctac taaagccgta tcaagtaaga gatggtgcat ccgggaggat tactcgcctt
      121 gcggctcgcc cttcgggccg ttgctaaagc aacgttatcc tccctggtgc ctgcggttat
      181 taccgccacc tcttccatac cttcatcgct gaaagtacgt agaatatggt gcatccggga
      241 ggattcgaac ctccgaccgc tcggttcgta gccgagtact ctatccagct gagctacgga
      301 tgcaaatggc ggtgaggcgg ggattcgaac cccggatgca gcttttgacc gcatactccc
      361 ttagcagggg agcgccttca gcctctcggc cacctcacca cacgcctctt acgagtgctt
      421 cgaagaactt gtttatcgct catcgtcgct gcgtggcgca catattactt tctgagactt
      481 ataagtcaaa caatttttcc cacactttta tcgtttgcac acttcacgct caattagtct
      541 gtaaaaacgt caaaaagagt gttttatcaa cagaaaaacg gaggtctgac agatagcagt
      601 aatgcaaaaa aatggagact taagttgaat gaacgggagt aaagcgaaaa gactatagag
      661 tgaaggagag attttgaggg tgcgtctcac cgataaagat gagacgcgga aagattagta
      721 actggactgc tgggattttt cagcctggat acgctggtag atctcttcac ggtgaacaga
      781 aacttccttc ggggcattta cgccaatacg tacctggttg ccctttaccc ctaaaactgt
      841 cacggtgacc tcatccccaa tcatgagggt ctcaccaact cgacgagtca gaatcagcat
      901 tctttgctcc ttgaaagatt aaaagagtcg ggtctctctg tatcccggca ttatccatca
      961 tataacgcca aaaagtaagc aatgacaaac acattacatg taagcagtca tggcattaca
     1021 ttctgttaaa cctaagttta gccgatatac acaacttcaa cctgacttta tcgttgtcga
     1081 tagcgttaat gcgaatgccg tgaagcgagt ccacggcatt gcctgacgct tatattattg
     1141 caatttcgcg ctgacccagc ctttcacact ggctaacgct gcaggtaagg ccgcagcatc
     1201 cgtaccaccg gcttgcgcca tgtcaggacg tccaccaccc ttgccgccca cctgctgagc
     1261 gaccatacca atcagttccc ctgctttcac acgatctgtg acgtccttag atacgcctgc
     1321 aatcagagaa accttacctt cggctaccgt tgccagcacg ataattgtcg accccagctg
     1381 attttttaaa tcgtcaacca tggtacgcaa cattttcggc tcaacaccgc taagctcgct
     1441 aaccaacagc ttaacaccat taacatcaat tgccttactg gaaagatttg cgctctcctg
     1501 tgcggcagct tgttctttaa gctgttgtag ttctttttct aactgacgcg tacgttccag
     1561 tactgagcgc actttatcag ccagattatt gctatcgcct ttcagcagat gcgcgacttc
     1621 gcttaagcga tcgctgtctg catgaacggt ggcgatagcg ccttctccgg ttaccgcttc
     1681 gatacgacga acgcctgcag cagtacccga ttcagagatg atgcggaaca gaccaatatc
     1741 accggtgcgg ctggcgtgag taccgccaca cagctcggtg gagaaatcgc ccatgctcag
     1801 cacgcgtacg cgctcatcat acttctcgcc gaacagcgcc atcgcacctt tcgctttcgc
     1861 cgcttcgaga tccatgatgt tggtttcgat cggcaagtta cggcgaatct gtgcgttcac
     1921 caggtcttcg accgcacgaa tctcttctgg tttcatcgct tcgttgtgtg agaagtcgaa
     1981 gcgcagcact ttgtcgttaa ccagtgaacc tttctgcgat acatgagtac ccagaacctg
     2041 gcgcagcgca gcgtgcatca ggtgcgttgc ggagtgattc agacgaatac gggcgcgacg
     2101 agcttcatca acatcagcct gcaccgcgtc gcccactttc agagaacccg cagcaagttt
     2161 accgatgtga ccaatcgcct ggccgtattt ctgcgtatct tccaccgcaa aggagaagtt
     2221 agcgcctttc agttcgcctt tatcaccaac ctgaccgccg gattccgcat agaatggcgt
     2281 ttgatccagc acgaccacag cttcctggcc tgcattgatg gcatcaaccg ctttaccatc
     2341 aacaaacagc gcggtcactt tgccgttcag ttccagatgg tcatagcctt taaattcaga
     2401 tgcgctgtca acacggatca ttgcgttgta atcggcacca aagccgctgg cttcgcgcgc
     2461 gcgacgacgc tgctcttcca ttgctgcttc gaaaccagct tcgtcaactt tgatgttgcg
     2521 ctcacgacaa acatcagccg tcaggtcaac cgggaagcca taggtgtcgt acagacggaa
     2581 agccgtttcg ccatccagcg tatcaccaga aagttttgcc agctcttcat ccagcagcgc
     2641 cagaccgcgc tccagagtac gggcaaactg ctcttcttca gtcttcagca cctgctcaac
     2701 ctgcgcctgc tggcgtttca ggtcttcacc cgcagagccc ataacgtcga tcagcggacc
     2761 aaccagtttg tagaagaagg tttctttcgc gccgagcata ttaccgtgac gcactgcgcg
     2821 acgaatgata cgacgcagta cataaccacg gttttcattg gacggcatta cgccatccgc
     2881 gatcaggaac gcacaagaac gaatgtggtc agcgattaca cgcagcgatt tgttgctcaa
     2941 atcggtcgcg ccagtgactt tcgctaccgc ctggatcagc gtacggaaca ggtcgatgtc
     3001 atagttagag ttaacgtgtt gcagcaccgc agcaatacgc tccagaccca taccggtatc
     3061 tacagacggc ttcggcagcg gttccatcgt gccatctgcc tggcggttga actgcatgaa
     3121 gacgatgttc cagatctcaa tgtagcggtc gccatcttct tccggacttc ccggagggcc
     3181 accccaaatg tggtcgccgt gatcgtagaa gatttcggtg cacgggccgc acggaccagt
     3241 gtcacccatc tgccagaagt tgtcagatgc gtatggcgca cctttgttat cgccgatgcg
     3301 aataatacgt tcgcgcggga tccctacttc tttttcccag atttcgtagg cttcgtcgtc
     3361 gctttcatag acggtaaccc acagacgctc tttcggcagg gcaaaccatt tttcactggt
     3421 cagcagttcc catgcaaact gaatggcatc gtgtttgaaa tagtcgccga agctgaagtt
     3481 gcccagcatt tcgaagaagg tatggtgacg cgcggtgtaa ccgacgtttt ccaggtcgtt
     3541 gtgtttacca cccgcacgca cgcagcgttg ggaagtggta gcgcgggaat aattacgctt
     3601 gtcgagccca aggaacacat ccttgaactg gttcatcccg gcgttggtaa acaacaaagt
     3661 tgggtcgtta tggggtacca gggagctgct ggcaactacc tgatgtccct tactatggaa
     3721 aaagtcgaga aacgcctgac ggatctcagc ggtgctcttg ctcataatta tcctgaaatc
     3781 aagctaacga aatatcgcta ccagccgttg cgtgtcgtta accgccgggc tggtaactga
     3841 aaagtgggaa taagataagt tttcttgact gggaagtaaa atcccgtatg cgttcagtcg
     3901 gcaaaatttc gccaaatatc ctggatatct tccatcagat agccacgata gagcagaaaa
     3961 cgctggatct taactttttc tgaaaagaca gttggcaaag gttcgccata ttttcgcgtc
     4021 gcctgatcgc gcgccagtgc gcaccagtcg atgtcacatt cacgcatcgc tttttctgtc
     4081 gcttcgcggg aaataccttt ctgattcagt tcctgacgaa tacgcgcagg tccataacct
     4141 ttgcggctac ggctggcgat aaagcgcgca acaaatcggc tgtcatcgag atagccatgt
     4201 tcatggcacc aggcgataac gcgctcgtaa tcttctgccg tggcatcaat ctcttccggg
     4261 ccatttttgc ccatgatcgg tgccgcgagt ttacgtcgca attcttgctc actgtgatcg
     4321 cgcaccgcca gaatgcgtac cgcacgatcc aacaggcgag catatgccgg gcgacgggac
     4381 gttgattctg tcatggcata tccttacaac ttaaaaaagc aaaagggccg cagatgcgac
     4441 ccttgtgtat caaacaagac gattaaaaat cttcgttagt ttctgctacg ccttcgctgt
     4501 catctacaga gaaatccggc gttgagttcg ggttgctcag cagcaactca cgtactttct
     4561 tctcgatctc tttcgcggtt tccgggttat ctttcagcca ggcagtcgca ttcgctttac
     4621 cctgaccgat cttctcacct ttgtagctgt accacgcacc tgctttctcg atcagcttct
     4681 ctttcacgcc cagatcaacc agttcgccat agaagttgat accttcgccg tagaggatct
     4741 ggaattcagc ctgtttaaac ggagcagcga ttttgttctt caccactttc acacgggttt
     4801 cgctacccac cacgttttcg ccctctttca ccgcgccgat acgacggatg tcgagacgaa
     4861 cagaggcgta gaatttcagc gcgttaccac cggtagtggt ttccgggtta ccgaacatca
     4921 caccaatttt catacggatc tggttgatga agatcagcag cgtgttggac tgcttcaggt
     4981 tacccgccag cttacgcatc gcctggctca tcatacgtgc cgcaaggccc atgtgagagt
     5041 cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc cgccacggag tcaacgacga
     5101 taacgtctac tgcaccagaa cgcgccaggg cgtcacagat ttccagtgcc tgctcgccag
     5161 tgtccggctg ggagcacagc aggttgtcga tatcgacgcc cagtttacgt gcatagattg
     5221 ggtccagcgc gtgttcagca tcgataaacg cacaggtttt accttcacgc tgcgctgcgg
     5281 cgatcacctg caacgtcagc gtggttttac cggaagattc cggtccgtag atttcgacga
     5341 tacggcccat cggcagacca cctgccccaa gcgcgatatc cagtgaaagc gaaccggtag
     5401 agatggtttc cacatccatg gaacggtctt cacccaggcg catgatggag cctttaccaa
     5461 attgtttctc aatctggccc agtgctgccg ccaacgcttt ctgtttgttt tcgtcgatag
     5521 ccatttttac tcctgtcatg ccgggtaata ccggatagtc aatatgttct gttgaagcaa
     5581 ttatactgta tgctcataca gtatcaagtg ttttgtagaa attgttgcca caaggtctgc
     5641 aatgcatacg cagtagcctg acgacgcacc gcatcacggt caccgctgaa gcattcccgc
     5701 cgggtaatac cttcgccgcg ggcggtggca aaagcaaacc agacggtgcc gacaggcttc
     5761 tcttcactgc cgccatccgg cccggcgata ccactaatag acacagcata atcagcacga
     5821 gccgctttca gtgcgcctat cgccatttcc accacgacag gttcgctcac cgcgccatgc
     5881 tgcgccagcg tctcttcgcg tacaccgatc atttgcgctt tcgcttcgtt actgtaggtg
     5941 acaaatccgc gttcaaacca ggcggaactg ccggcaatat cggtaatcac tttcgctacc
     6001 caaccaccgg tacaagactc agcagttgtt acggttgcgc cacgggcttt cagcgcctgc
     6061 ccaacctgtt cgcttaactg catcagttca ctgtcagtca tcacaacctc tgtcagtcaa
     6121 aaatttatgc cggacaagat agcactttcg tcaggaagat ggtgttggcg ttgcaatttt
     6181 acgaggagga ttcagaaaaa agctgattag ccagagggaa gctcacgctc ccctcttgca
     6241 aatagttact gcacgcgcgc cagcgccacg gcttgtccta actgccagac cgccattgcg
     6301 taatgggtgc tgtggttata acgggtaatg gtgtagaagt tcggcagacc gtaccagtac
     6361 tggtaacctg ttccaacatc cagacgcagc aggctggctt gttgatggtt gcccagcggc
     6421 tgctgtggcg ttaaacctgc cgcggtaagc tgcgaaatgc tgtacttggt tttgaagcca
     6481 tttggcaagc ctggagcctg accgtttgcc attaccgcga cctgatcgcc tttcacccag
     6541 ccgtgcgctt tgaaatagtt cgccacgcta ccgatcgcat caaccggatc ccacaggttg
     6601 atatgcccgt cgccgctgaa atctaccgca tattgtttgt aagacgacgg cataaactgt
     6661 ccgtagccca tcgccccggc aaaggaacct ttcagattga gcggatcgtc ctgctcatcg
     6721 cgcgccatca gcaggaaagt ttccagctcg ccagagaaat actccgcgcg gcgtgggtag
     6781 ttaaatgaca gcgttgccag cgcatcgagg atacgggttt tccccatcac gcgcccccag
     6841 cgggtttcaa cgccgataat cccgacgata atttccggcg gtacgccata cacctgccac
     6901 gcgcgattca acgcatcttc atactgattc cagaaaacca caccattctg cacgttgtcc
     6961 ggcgtaataa attttttgcg ataacgcagc catgcgccgt tcggacctga tggcggtttc
     7021 accgatgtgg ttggtgcctg gttatccatc agccgcagta ccgaatccag acgtttcgcc
     7081 tgagagagaa tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac cattttgtcg
     7141 atgaactgct gggcattcgg gttattggcg aagtcaccgc ccatctgcat cacattgtgc
     7201 tgcggctcaa gcaggaagcc gccagacggc gttccggtgg tcgtttcagt ctcagtaggt
     7261 tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt tacataacga
     7321 cgcttgaaca tgaggggtcc atttaacaga ttcaaccaag ggcaagtatg gtaaagcatc
     7381 acgccccgca caaggaagcg gtagtcactg cccgatacgg actttacata actcaactca
     7441 ttctcctcgc tatcctttta ttcaaacttt caaattaaaa tatttatctt tcattttgcg
     7501 atcaaaataa cacttttaaa tctttcaatc tgattagatt aggttgccgt ttggtaataa
     7561 aacaataaat cctgaaggag agaacaatga tagaaaccat tactcatggt gcagagtggt
     7621 ttatcgggct gttccaaaag ggcggagagg tgtttaccgg gatggtgacc ggcattcttc
     7681 cgctgttgat tagtctgctg gttatcatga acgcactgat taattttatc ggtcagcatc
     7741 gtattgaacg ttttgctcaa cgttgcgccg gtaaccctgt ttcccgttac ctgctgctac
     7801 cgtgcattgg tacgtttgtc ttttgtaatc caatgaccct aagcctgggt cgctttatgc
     7861 cggaaaaata caaacccagc tactacgcag cggcctctta tagctgccac tccatgaatg
     7921 gcctcttccc tcatatcaac cctggcgaac tgtttgttta tcttggcatt gccagcggtc
     7981 tgacgacgct gaatctgcca cttggcccac tggcagtgag ttatctgctg gttggtttgg
     8041 tcaccaactt cttccgcggc tgggtgaccg atctgaccac cgccattttt gagaaaaaga
     8101 tgggtattca acttgaacaa aaagttcacc tggcaggagc aacatcatga cgcgtattcg
     8161 gatcgaaaaa ggaacgggtg gctggggcgg cccgcttgag ctggaagcca cgccgggcaa
     8221 aaaaatcgtc tatatcaccg ccggtacccg gcctgcgatt gttgacaaac tggcacagct
     8281 tactggctgg caggctattg acggatttaa agaaggtgaa cccgcggagg cggaaattgg
     8341 tgtcgcggta atcgactgtg gcggcacatt acgctgcggc atctatccga aacgacgtat
     8401 tcccaccatt aatatccact cgacgggcaa gtccggtcca ctggagcagt acattgtaga
     8461 agatatttat gtctctggcg taaaagaaga aaacatcact gtagtgggtg atgcgacacc
     8521 acaaccctct tccgtgggcc gtgactatga caccagcaag aaaatcaccg aacaaagcga
     8581 tggtttactg gcgaaggtgg gaatgggtat gggttctgcc gttgccgtgt tgtttcaatc
     8641 tggtcgtgac accatcgaca ctgtattaaa aaccattctg ccgtttatgg cgttcgtctc
     8701 ggcgcttatc ggcatcatta tggcttccgg tcttggcgac tggattgccc acggtcttgc
     8761 tccgctggct agccatcctc tggggctggt catgctggcg ctcatctgct ccttcccgct
     8821 gctttcacct ttcctcggcc caggcgcagt tatcgcacag gttatcggcg tattgattgg
     8881 cgtgcagatt ggtctcggca atattccgcc gcatctggct ttacctgcac tgtttgccat
     8941 caacgcgcag gcggcctgcg acttcatccc ggtcggtttg tcgctggcgg aagcccgtca
     9001 ggacacggtt cgcgtcggtg tcccttctgt tctggtgagc cgctttttaa ccggcgcgcc
     9061 aactgtactg atcgcctggt ttgtctccgg ttttatctat caatagaggc tgaaacatga
     9121 ccgttattta tcagaccacc atcacccgta tcggcgcgag tgccactgac gccctcagcg
     9181 accagatgct catcaccttt cgtgaaggcg cgcctgcgga tctcgaagag tattgcttca
     9241 ttcattgcca cggcgagttg aaaggtgcac tccatcccgg tttgcaattt tcgctcgggc
     9301 agcatcgcta tccggtgacc gccgttggca gcgtggcgga agacaacctt cgcgaactgg
     9361 gtcatgtcac cctgcgcttc gatggtttaa acgaagcgga attcccgggc actgtccatg
     9421 tggcaggccc tgtacccgac gatatcgcgc cgggatcggt tttgaagttt gaatctgtta
     9481 aggagtaaaa aatgaatcag gttgccgttg ttatcggtgg tgggcaaacc ttaggcgcgt
     9541 tcctgtgcca cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg
     9601 acaaagccgc aaatgtggca caagaaatta acgccgaata tggtgaaggt atggcgtacg
     9661 gttttggtgc tgacgccacc agtgagcaaa gcgttctggc gctctctcgt ggggtagatg
     9721 aaatctttgg tcgcgtggat ttgctggtct acagcgccgg aattgcaaaa gcagccttta
     9781 tcagcgactt ccagctcggc gattttgacc gttcgctaca agtgaatctg gtgggttatt
     9841 tcctgtgtgc ccgtgaattt tcgcgtttga tgatccgcga cgggattcag gggcgcatta
     9901 ttcagatcaa ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggt tatagcgccg
     9961 cgaaatttgg tggcgtcggg ctgactcaat cactggcgct ggatctggcg gagtacggca
    10021 ttacggtgca ttcactgatg ctcggtaatc tgctgaaatc gccgatgttc cagtcgctgt
    10081 tgccacaata cgcgaccaag ctgggtatca aaccggatca agtcgagcag tattacatcg
    10141 ataaagtacc gctcaaacgc ggctgcgatt atcaggatgt actgaatatg ctgctgttct
    10201 acgccagtcc taaggcgtcg tactgcactg gtcagtcgat caatgtcacc ggcggtcagg
    10261 tgatgttctg atcaacagcg gagatccgtt aaggatctcc
//
LOCUS       DAPOZO010000141         9940 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPOZO010000141 DAPOZO010000000
VERSION     DAPOZO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.141"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            53..2653
                     /gene="recE"
                     /locus_tag="ROI21_004291"
     CDS             53..2653
                     /gene="recE"
                     /locus_tag="ROI21_004291"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HDX5113140.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNSGKEEE
                     KAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETA
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            2646..3455
                     /gene="recT"
                     /locus_tag="ROI21_004292"
     CDS             2646..3455
                     /gene="recT"
                     /locus_tag="ROI21_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HDX5113141.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            3511..3660
                     /gene="ralR"
                     /locus_tag="ROI21_004293"
     CDS             3511..3660
                     /gene="ralR"
                     /locus_tag="ROI21_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HDX5113142.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
     gene            3698..3886
                     /locus_tag="ROI21_004294"
     CDS             3698..3886
                     /locus_tag="ROI21_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HDX5113143.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            3986..4201
                     /gene="xisR"
                     /locus_tag="ROI21_004295"
     CDS             3986..4201
                     /gene="xisR"
                     /locus_tag="ROI21_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HDX5113144.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            4203..5438
                     /locus_tag="ROI21_004296"
     CDS             4203..5438
                     /locus_tag="ROI21_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDX5113145.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEVTNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGVVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            5490..6425
                     /gene="ttcA"
                     /locus_tag="ROI21_004297"
     CDS             5490..6425
                     /gene="ttcA"
                     /locus_tag="ROI21_004297"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDX5113146.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREELPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(6554..7927)
                     /gene="dbpA"
                     /locus_tag="ROI21_004298"
     CDS             complement(6554..7927)
                     /gene="dbpA"
                     /locus_tag="ROI21_004298"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDX5113147.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(8405..9388)
                     /gene="zntB"
                     /locus_tag="ROI21_004299"
     CDS             complement(8405..9388)
                     /gene="zntB"
                     /locus_tag="ROI21_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDX5113148.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            9643..>9940
                     /locus_tag="ROI21_004300"
     CDS             9643..>9940
                     /locus_tag="ROI21_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HDX5113149.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAE"
BASE COUNT         2887 a         2379 c         2373 g         2301 t
ORIGIN      
        1 agatcattca ctgaacaaaa cgaattttaa tctgagttga ggttaaaaaa caatgagcac
       61 aaaaccactc ttcctgttac ggaaagcgaa aaaatcatcc ggtgaacctg acgtcgtcct
      121 gtgggcaagc aacgattttg aatcgacctg tgccactctg gactacctga tcgttaagtc
      181 aggtaaaaaa ctgagcagct attttaaagc tgttgccacg aattttcctg tcgttaatga
      241 cctgcccgct gaaggtgaga tcgattttac ctggagtgaa cgctatcaac tcagcaaaga
      301 ctccatgaca tgggaactaa aaccgggagc agcaccagac aacgctcact atcaaggcaa
      361 taccaacgtc aacggcgaag acatgactga gattgaggag aatatgctac tcccaatttc
      421 tggccaggaa ctgcccattc gttggcttgc tcaacacggc agcgaaaaac cggtaacgca
      481 cgtttcacgc gacggactcc aggcattaca cattgctcgg gctgaagaac taccggctgt
      541 tactgccctg gctgtttccc acaaaaccag cctgctcgac ccgctggaaa ttcgcgaact
      601 ccacaaactg gttcgtgaca ctgacaaagt tttccctaat cctggtaatt caaacctggg
      661 actgataact gcttttttcg aagcatacct gaacgctgac tacaccgatc gaggactgct
      721 gacaaaagag tggatgaagg gtaatcgtgt ttcacacatc actcgcacgg cttccggtgc
      781 taatgctggc ggcggaaacc tcaccgatcg cggcgaaggt ttcgtacacg atctgacgtc
      841 actggcgcgc gacgtagcca ctggcgtact ggcccgttca atggatctgg acatctataa
      901 ccttcatccg gcacacgcta aacgcattga ggaaattatc gctgaaaata aaccgccctt
      961 ttctgttttc cgcgacaaat tcatcaccat gcctggcggg ctggattatt cccgcgccat
     1021 cgtggttgcg tccgtaaaag aagcaccaat tgggatcgag gtcatccccg cgcacgtcac
     1081 tgaatatctg aacaaagtac tgactgaaac cgatcatgcc aaccctgatc cggaaatcgt
     1141 ggatattgcc tgcggtcgct cctctgcccc gatgccgcag cgagtaacag aagaaggaaa
     1201 acaggatgat gaagaaaaac cgcaaccatc tggaacaacg gcagttgaac agggagaggc
     1261 tgaaacaatg gaaccggacg caactgaaca tcatcaggac acgcagccgc tggatgctca
     1321 gtcacaggta aattctgttg atgcgaaata tcaggaactg cgggcagaac tccatgaagc
     1381 ccggaaaaac attccatcaa aaaatcctgt cgatgccgat aaattgcttg ctgcatcacg
     1441 tggtgaattt gttgacggaa ttagcgaccc gaacgatccg aaatgggtaa aggggatcca
     1501 gactcgcgat tgtgtgtacc agaaccagcc agaaacggaa aaaaccagcc cggatatgaa
     1561 tcaacctgag ccagtagtgc aacaggaacc ggaaatagcc tgcaatgcct gcggccagac
     1621 tggcggggat aactgccctg actgtggtgc ggtgatgggc gacgcaacat accaggaaac
     1681 attcgatgaa gagagtcagg ttgaagctaa ggaaaatgat ccggaggaaa tggaaggcgc
     1741 tgaacatccg cacaatgaga atgctggcag cgatccgcat cgcgattgca gtgatgaaac
     1801 tggcgaagtc gcagatcccg taatcgtaga agacatagag ccaggtattt attacggaat
     1861 ttcgaatgag aattaccacg cgggtcccgg tgtcagtaag tctcagctcg atgacattgc
     1921 tgatactccg gcactgtatt tgtggcgtaa aaatgccccc gtggacacca caaagacaaa
     1981 aacgctcgat ttaggaaccg ctttccactg ccgggtactt gagccggaag aattcagtaa
     2041 ccgctttatc gtagcacctg aatttaaccg ccggacaaac tccggaaaag aagaagagaa
     2101 agcgtttctg agggaatgcg caagcacagg aaaaacggtt atcactgccg aagaaggccg
     2161 gaaaattgaa ctcatgtatc agagcgttat ggctttgccg ctggggcaat ggcttgttga
     2221 aagcgccgga cacgctgaat catcaattta ctgggaagat cctgaaacag caattttgtg
     2281 tcggtgccgt ccggacaaaa ttatccctga atttcactgg atcatggacg tgaaaactac
     2341 ggcggatatt caacgattca aaaccgctta ttacgactac cgctatcacg ttcaggatgc
     2401 attctacagt gacggttatg aagcacagtt tggagtgcag ccaactttcg tttttctggt
     2461 tgccagcaca actattgaat gcggacgtta tccggttgaa attttcatga tgggcgaaga
     2521 agcaaaactg gcaggtcagc tggaatatca ccgcaatctg cgaaccctgg ctgactgcct
     2581 caataccgat gaatggccag ctattaagac gttatcactg ccccgctggg ctaaggaata
     2641 tgcaaatgac taagcaacca ccaatcgcaa aagccgatct gcaaaaaact cagggaaacc
     2701 gtgcaccagc agcaattaaa aataacgacg tgattagttt tattaaccag ccatcaatga
     2761 aagagcaact ggcagcagct cttccacgcc atatgacggc tgaacgtatg atccgtatcg
     2821 ccaccacaga aattcgtaaa gttccggcgt taggaaactg tgacactatg agttttgtca
     2881 gtgcaatcgt acagtgttca cagctcggac ttgagcccgg tagcgccctc ggtcatgcat
     2941 atttactgcc ttttggtaat aaaaacgaaa agagcggtaa aaaaaacgtt cagctaatca
     3001 ttggctatcg cggcatgatt gatctggctc gccgttcagg tcaaatcgcc agcttgtcag
     3061 cccgtgttgt ccgtgaaggt gacgagttta atttcgaatt tggccttgat gaaaagttaa
     3121 tacaccgccc aggagaaaac gaagatgccc ctgttaccca cgtctatgct gtcgcaagac
     3181 tgaaagacgg aggtactcag tttgaagtta tgacgcgcaa acagattgag ctggtgcgca
     3241 gccagagtaa agctggtaat aacgggccgt gggtaactca ctgggaagaa atggcaaaga
     3301 aaacggctat tcgtcgcctg ttcaaatatc tgcccgtatc aattgagatc cagcgtgcag
     3361 tatcaatgga tgaaaaggaa ccactgacaa tcgatcctgc agattcctct gtattaaccg
     3421 gggaatacag tgtaatcgat aattcagagg aataattcag cctggcggtg taatgcaccg
     3481 ccaacttgaa atatttttat gagaaaaatt atgagatatg acaatgttaa accatgtcca
     3541 ttttgtggtt gtccatcagt aacggtgaaa gccatttcag gatattaccg agcgaagtgt
     3601 aacggatgcg aatcccgaac cggttatggt ggaagtgaaa aagaagcact cgaacgatga
     3661 aataaacgaa ccactggaaa taataatgga ggtgttcatg tataaaatta ccgccactat
     3721 tgaaaaggaa ggtggcactc ctactaactg gacaagatat tcaaaatcta aactaacgaa
     3781 atcagaatgc gaaaaaatgc tctcaggtaa aaaagaagca ggcgtttcca gagagcagaa
     3841 agtaaaactg ataaatttta attgcgagaa acttcagtcc tcgtgaattg cattgtattc
     3901 aaattaaaac ttcatagctg attattaata atcaacatcg ggcgtcaatt taagtctaac
     3961 attggcgcct gccagaggtg atgcgatggc acaagtaatc tttaatgaag agtggatggt
     4021 tgaatacggc ctgatgcttc gcactggtct gggggccaga caaattgaag cataccgcca
     4081 gaactgttgg gtggaaggct tccacttcaa acgagtatct cctttaggga agccagacag
     4141 taagcgaggg attatctggt ataactatcc aaagataaat cagtttatca aagactcatg
     4201 atatgtctaa attaccaaca ggtgtcgaga ttcgaggtaa atacattcgc atctggttca
     4261 tgtttcgagg aaaacgatgt cgggaaacat tgaaaggctg ggaggttact aacagtaaca
     4321 ttaaaaaagc cgggaattta agagcgttga tagttcatga aatcaattcc ggtgagtttg
     4381 agtatttaag acgttttccc cagtccagca ctggggcaaa aatggtgaca acgagggtca
     4441 taaaaacgtt cggggagctt tgtgatatct ggacaaaaat taaagaaaca gagttaacaa
     4501 caaacacaat gaagaaaacg aaatcacaat taaaaacact caggataata atttgtgaga
     4561 gtactccgat atcgcatatt cgttatagcg atatcttaaa ctaccggaat gaactgctgc
     4621 atggagaaac gctttacctg gataatccaa gatccaacaa aaaaggaaga accgtgcgca
     4681 cagttgataa ctatatcgcc ctgctctgtt cgttgttacg ttttgcgtat cagtcgggat
     4741 ttatatcaac caaaccattt gaaggagtaa agaaattaca gagaaacaga ataaagcctg
     4801 acccgttatc taaaacagaa ttcaatgcat taatggaaag tgaaaaagga cagagccaga
     4861 acttgtggaa atttgccgta tactccgggc ttcgtcacgg ggaactggct gctctggcgt
     4921 gggaggatgt ggatttcgag aagggagttg tgaatgtcag aagaaacctg acgatactgg
     4981 atatgttcgg tcccccaaaa acaaatgcgg ggatccggac ggtaacacta ctgcagcctg
     5041 ctcttgaagc actgaaggag caatacaaac tgaccgggca tcatcgcaaa agcgaaatca
     5101 ccttttatca tcgggagtac ggcagaaccg aaaagcaaaa actgcatttt gttttcatgc
     5161 ccagggtgtg taacggaaaa caaaaacctt attactcggt aagcagtttg ggggcaaggt
     5221 ggaatgcagc agtaaaacgt gctggtattc gccgccgtaa tccgtaccat acgcggcata
     5281 cttttgcctg ctggctgttg acggcaggag cgaacccggc atttatagcc agccaaatgg
     5341 ggcatgaaac tgcgcagatg gtgtatgaaa tttacggtat gtggattgat gacatgaacg
     5401 acgaacaggt agccatgttg aatgcgcggt tatcatagtt gcaaagtttg cccccaattt
     5461 gccccattta gtaccagaga actgaaataa tgcaagaaaa tcaacaaatt acaaagaaag
     5521 aacaatacaa cctgaacaaa ttacaaaaac gtctgcgtcg taacgtgggc gaagccattg
     5581 ctgacttcaa tatgattgaa gaaggcgatc gcatcatggt ttgcctctcc gggggtaaag
     5641 acagctatac catgctggaa attctgcgca atttgcagca aagcgcgcca atcaattttt
     5701 cgctggtggc tgtcaacctc gatcaaaaac aaccgggctt cccggaacac gttctgcccg
     5761 agtatcttga aaagctgggc gttgagtaca agattgtgga agagaacact tacggtatcg
     5821 tgaaagagaa gattccggag ggcaaaacca cttgctcact gtgttctcgt cttcgtcgcg
     5881 gaattcttta ccgtaccgca acggaactgg gagcgacgaa gatcgcgctg ggtcaccatc
     5941 gtgacgatat cctgcaaacg ttgttcttaa atatgttcta cggcggcaag atgaaaggta
     6001 tgcctccgaa actgatgagc gatgatggca aacatattgt gattcgtccg ctggcctact
     6061 gccgcgagaa agatattcag cgctttgccg atgcaaaagc attcccgatt attccgtgca
     6121 atctgtgcgg ttcacagcct aacctgcaac gtcaggtgat tgctgacatg ttgcgtgact
     6181 gggataaacg ttatccaggg cgtatcgaga cgatgttcag cgcgatgcag aatgtggtgc
     6241 cgtcgcatct gtgcgatacc aacctgttcg atttcaaagg cattacccac ggttctgaag
     6301 tggttaacgg gggtgatctg gcgtttgatc gcgaagagct ccctctacaa ccggcgggtt
     6361 ggcagcctga agaagatgaa aatcagttgg atgagttacg actgaatgtg gttgaagtga
     6421 aataacccgg atagcgcccg atgcgcaagc ttatcgggct actcttatgg aggccggata
     6481 agacgcggcc agcgtcgcat ccggcaatcc cgaataagat gtttactctt gcaccctgcg
     6541 attcaacatt tcattatttt aataaccgca cccggcacgt ttttccttta atcttcccgc
     6601 cctgtaactg tttccatgct ttatgagcaa cagcctgacg caccgcgaca tagacatgcg
     6661 ccggatgcac ggcgattttg ccaatatctg cgccatcaag cccgatatct cctgtcagcg
     6721 cacctaatac atcacccggg cgcattttgg cttttttccc gccatcgata cataacgttg
     6781 ccatttcggc ttccagcggc acaatggaac tattaactgg cggcgtttgc cagttaagtt
     6841 ttatctgcaa catgtcagaa atgatattgg cccgctgtgc ttcttccggg gcacagaaac
     6901 tgatcgccag accgctattt cctgcacgcg ctgtacgacc gatgcgatgt acatgaactt
     6961 caggatccca cgccagctca aagttcacca ccagctcaag cgatttaata tccagacctc
     7021 gcgcagcaac atcagtcgca accagtacac gggcgctacc gttagcaaaa cgtaccaggg
     7081 tctgatcgcg atcacgttgc tccagatcgc cgtgtaacga caatgcactt tgccctactt
     7141 cattcagcgc gtcgcagaca gcctggcaat cttttttggt attgcaaaac accacgcaag
     7201 aggatggctg atgtaaactt aataaccgtt gcaacaacgg aattttgcct ttgctggatg
     7261 tctcataaaa ttgttgttca ataggtggca gagcatctgt tgagtcaatt tcaatcgcta
     7321 aaggatcgcg ttgcactcgt ccgctgattg cggcgatggc ttccggccag gttgccgaaa
     7381 acagaagcgt ctgtcgagat gctggcgcaa aacggatgac atcatcaatg gcgtcgctaa
     7441 atcccatatc cagcatgcgg tcggcctcat ccatcaccag cgtattcaac gcatccagtg
     7501 ataccgtgcc tttttgcaga tgatccaaca aacggcccgg cgttgccacg ataatatgcg
     7561 gcgcatgttg caacgaatca cgctgcatac cgaacggttg accaccgcac aacgtcaaaa
     7621 ttttggtatt tggtagaaaa cgcgccagcc gacgcaattc acctgccacc tgatccgcca
     7681 gttcacgcgt aggacacagc actaaagcct gggtttgaaa tagcgacgca tcaatttgct
     7741 gtaacaagcc gaggccaaaa gccgccgttt tgccgctgcc ggttttcgcc tgcacgcgaa
     7801 catcttttcc ggcaaggatc gccggaagcg cggcggcctg caccggcgtc atggttaaat
     7861 aacccaactc attaaggttc gtgagttggg cgggaggcaa aacattcagg gtagaaaaag
     7921 cggtcacaat ctattctcgt ggtcatcgac gcaaagttag caggcgcgta tcctcgcaga
     7981 tctacgctca cgatgcgaca atttaatcgg ttcttcatcg ggtggtgggt caggcatggg
     8041 ttgcgggcga gggatcggat cgggcactgg aacaggatcg ccaggaatcg gttcagggac
     8101 aggaatttgc aaataaataa gtgtcgtcat atttccctcc ggtcattggg tggactctta
     8161 aagggtagac gctgataaat aacaggcaaa aaaaagccga ctcatcaaag tcggcgtcgt
     8221 acgaatcaat tgtgctatgc agtaattcaa aaaaggaagt aagacaatat ggagcgcaac
     8281 gcccatcgct tgacgttgca ttcacctgca agagagatat tgccctgaat gggtagagag
     8341 tttattgact tcgctcaaac tttgcggcgt ttttgtatac agatagccgg aaaaattgct
     8401 tttgttacaa ccatttacta cgatgcaacc ataaagcaac accaccaata agaacaacta
     8461 acagaataca aaaaattgaa aatccgaatt gccacccgcc gccagggatc ccaccaaggt
     8521 tgacgccaaa taacccggtc agaaaggtac tgggtaaaaa gaccattgcc atcaacgaca
     8581 ttgtataggt acgacgcgct aaattttcct gcatcacctg agcgatttca tcagccatca
     8641 cgccagtccg tgctatacag gcgtcgattt cgtcaagccc gcgcccaagg cgatcggcaa
     8701 tatcctgcat acgacggcgt tggtcatcgc tcatccacgg caaacgttca ctggcaagac
     8761 gagcataaac atcacgttgc ggtgccatat agcgacgcat cacaattaat tgtttgcgca
     8821 gcagagccag gaatccacgc ggtggaattt gctgatcaag gagattatct tcaaggtcga
     8881 taattttatc gtgcagctgc tcaataaatt cgctggaatg atcggtcaac gcatcacata
     8941 catccaccag ccatcccccg caatcggtcg gacccgtgcc ctcttccaga tcgcttacca
     9001 catcgtccag cgccagcact ttgcgttgtc gggtcgaaac aattaaccgc ccgtccatat
     9061 atacacgcat ggcgaccagt tgatcggggc gttcatcggt gctgccgttt atacagcgca
     9121 atgtaatcag cgtgccttca ccgagacggc tgactcgggg ccgtgtgctc tcgcccgcca
     9181 gcgcatcacg tacgttattg ggaagcagcg gtgttgtcgc cagccattgg gcgctatcat
     9241 ggtgtacata attaaggtgg agccagcagg gatgcgcttc atcaatcaca tctgtatttt
     9301 ccagcggttt aacgccgcct ctaccatcca gcatccaggc aaatactgca tccgggacat
     9361 taacgtccga tcccttaatc gcttccacag tgcctccatc atcaacgcat tattttgtag
     9421 tctagccttc tggccctgtt acgcaacatc tcatcacccc attaccctga aatgattaat
     9481 aaaattctgt ctaaattgaa tacaaaaagc aaaatgcttt tccatataca aaccgtgtga
     9541 agtgttaaat agcgtctatc attatcagaa ttatctgatc atatgacgtg gcttttttgc
     9601 gatcggatag caacaaaaat tgataaaaat aacgggatct caatgattac gcacaacttc
     9661 aataccctgg acttactcac cagtcctgtc tggatcgttt cgccctttga ggaacagtta
     9721 atttatgcca acagcgcggc gcgactgttg atgcaagacc tcacgtttag tcagctacga
     9781 accggtccct attccgtctc ctcacaaaaa gaactgccga aatacctctc cgatctgcaa
     9841 aaccaacacg atattatcga aatcctcacc gttcagcgca atgaagagga aacagcattg
     9901 agctgtcggc ttgttttgcg aaagctgaca gaagcagaac
//
LOCUS       DAPOZO010000142         9784 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPOZO010000142 DAPOZO010000000
VERSION     DAPOZO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.142"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            1..1111
                     /locus_tag="ROI21_004301"
                     /pseudo
     CDS             1..1111
                     /locus_tag="ROI21_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1611..3701
                     /gene="iha"
                     /locus_tag="ROI21_004302"
     CDS             1611..3701
                     /gene="iha"
                     /locus_tag="ROI21_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HDX5113150.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPNLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(3919..4078)
                     /locus_tag="ROI21_004303"
                     /pseudo
     CDS             complement(3919..4078)
                     /locus_tag="ROI21_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            4563..4805
                     /locus_tag="ROI21_004304"
     CDS             4563..4805
                     /locus_tag="ROI21_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113151.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            5096..5464
                     /locus_tag="ROI21_004305"
     CDS             5096..5464
                     /locus_tag="ROI21_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113152.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTEDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQYSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            5468..5683
                     /locus_tag="ROI21_004306"
     CDS             5468..5683
                     /locus_tag="ROI21_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113153.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            6011..6094
                     /locus_tag="ROI21_004307"
                     /pseudo
     CDS             6011..6094
                     /locus_tag="ROI21_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
     gene            complement(6237..6665)
                     /locus_tag="ROI21_004308"
     CDS             complement(6237..6665)
                     /locus_tag="ROI21_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HDX5113154.1"
                     /translation="MKKFINVYIKSDYDIVILRLSVIIIFMLFGTYKWFDFEVMALKP
                     LISPTWLNILYIMFGVHGGSYFLGIVETITFLALSIGFFRPAAGILGDIIVIVTGLVT
                     LSLLPQLGKVDSFVIKDVLLLGAGSVLLKYDLKNHLNIKN"
     gene            complement(6705..7265)
                     /locus_tag="ROI21_004309"
     CDS             complement(6705..7265)
                     /locus_tag="ROI21_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDX5113155.1"
                     /translation="MSTYENILRITDTLIQQRGFLGFSYADLEKEIGIRKASIHHHFP
                     RKTDLGIAYCQYKTEVFDRLNITLHNVSSGVQRLRTYLDAFAGCAERGEMCGIYAMLS
                     DSHQFSPELQEAVSQLAHQEIQMIKDIITSGQNSGEFKTVLLPDELAVIVCSTLKGAL
                     MLNRLPPHDTYSGTVNALIKMLDTRT"
     gene            complement(7307..7567)
                     /locus_tag="ROI21_004310"
     CDS             complement(7307..7567)
                     /locus_tag="ROI21_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDX5113156.1"
                     /translation="MNYVICTTIVALGLILTGYATAADEINRQDVVGLMPSDYISVGN
                     LYTPEEVATALKDKAEQAGGSHFRITSLGGKNKLDGTAIIYR"
     gene            8116..8857
                     /locus_tag="ROI21_004311"
                     /pseudo
     CDS             8116..8857
                     /locus_tag="ROI21_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9393..9506
                     /locus_tag="ROI21_004312"
     CDS             9393..9506
                     /locus_tag="ROI21_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDX5113157.1"
                     /translation="MKMKHLRDDVAAGRRRLYIRAICPSLREAPLLYSYPL"
     gene            9597..>9784
                     /locus_tag="ROI21_004313"
     CDS             9597..>9784
                     /locus_tag="ROI21_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113158.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQDEYDSQRAAICSIAPKTGCTP
                     ETLRVWIRQHERDTGGGDG"
BASE COUNT         2643 a         2219 c         2473 g         2449 t
ORIGIN      
        1 ggtgtggcag ccagccagtt atttctctgg cgcaagcaat accaggaggg aagtcttagt
       61 gctgtggccg caggagaaca ggtcgttcct gcctctgaac ttgctgctgc catgaagcag
      121 attaaagaac tccagcgcct gctcggtaaa aaacgatgga aaatgaactc cttaaagaag
      181 ccgttgaata tgggcgtgca aaaaagtgga tagcgcacgt gcccttattg cccggggatg
      241 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      301 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      361 gcttctccgt atataccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      421 ggcgctgctt cgcagacagg ccgaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      481 ttaccggatc atgcgccaga atgcgctgtt gcttgagcga aaaaccgctg taccgccatc
      541 gaaacgggca catactggca aagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      601 cgggttcgag ttccgctgtg ataacggaga aaaactgcga gtcacgttcg cgctggactg
      661 ctgtgaccgt gaggcactgc actgggcggt cactacgggc ggcttcgaca gtgaaacagt
      721 acaggacgtc atgctgggag cggtggaacg ccgcttcggc aacgagcttc cggcgtctcc
      781 agtagagtgg ctgacggata atggttcatg ctaccgggct aatgaaacac gccagttcgc
      841 ccggatgttg ggacttgaac cgaagaacac ggcggtgcgg agtccggaga gtaacggcat
      901 agcagagagc ttcgtgaaaa cgataaagcg tgactacata agtatcatac ccaaaccaga
      961 cgggttaacg gcagcaaaga accttgcaga ggcgttcgag cattataacg aatggcatcc
     1021 gcatagtgcg ttgggttatc gctcgccacg ggaatatctg cggcagcggg cctgtaatgg
     1081 gttaagtgat aacagatgtc tggaaatata ggggcaaatc cattgtcagc gccaacaatg
     1141 tgggctgaca tgataaagct gtctaggaaa tcgttcgcct cctcagcgga caatccaaat
     1201 ggtgattgtc tctgttaaac gtttattttg aaggtcgact gaataaggtg atgacgctgt
     1261 agaatttttc acgtgccaca gaattttgaa cgctttctct tacaatattt caatgtttct
     1321 atcagtattc gccggaagaa gtcatcgacc aaatcatccc agtcgtctcg caccactgac
     1381 cattcatggt tgacatgtgg tggaaatccg cttctacagt aaccattttt tattcgcaaa
     1441 accgaacacg ccatacggat agctgttaac tggcatgccg aggcagtcgt tatttatatt
     1501 tagttttgtc aataatcttt attttttgta aaaggtaaat ataaattatt ctcattattg
     1561 tttgtatttg tgtattgtct tgccggttaa catgatcgga gattagtaat atgcgaataa
     1621 ccactctggc ttccgtagtc attccctgtc tcggattttc agccagcagc atagctgctg
     1681 cagaggatgt gatgattgtc tcggcatccg gctatgagaa aaagctgact aacgcagccg
     1741 ccagtgtttc tgtgattagc caggaggaat tgcagtccag ccagtaccac gatctggcgg
     1801 aggctctgag atcagtagag ggtgtggatg ttgaaagtgg tacgggtaaa accggtgggc
     1861 tggaaatcag catccgagga atgccagcca gttacacgct gatactgatt gatggtgttc
     1921 gtcagggcgg aagcagtgac gtgactccca acggtttttc tgccatgaat accgggttca
     1981 tgccccctct ggccgccatt gagcgtattg aggttatcag ggggccgatg tccacactgt
     2041 atggctctga tgcgatgggc ggtgtggtga atatcattac cagaaagaat gcagacaaat
     2101 ggctctcttc cgtcaatgca gggctgaatc tgcaggaaag caacaaatgg ggtaacagca
     2161 gccagtttaa tttctggagc agtggtcccc ttgtggatga ttctgtcagc ctgcaggtac
     2221 gcggtagcac acaacagcgt cagggctcat cggtcacatc actgagcgat acagcagcca
     2281 cgcgtattcc ttatcccacg gagtcacaga attataatct tggtgcacgt cttgactgga
     2341 aggcgtcgga gcaggatgtg ctctggtttg atatggatac cacccggcag cgttatgata
     2401 accgggatgg gcaactgggg agtctgacgg ggggatatga ccggaccctg cgctatgagc
     2461 gaaacaaaat ttcagctggc tatgatcata ctttcacctt cggaacatgg aaatcgtatc
     2521 tgaactggaa cgagacagaa aataaaggtc gtgagctcgt acgcagtgta ctgaagcgcg
     2581 acaaatgggg gcttgccggt cagccgcggg agcttaagga atcgaacctt atcctgaatt
     2641 cattactgct tacccctctg ggagaatctc atctggttac ggtggggggc gagtttcaga
     2701 gctcgtccat gaaagacgga gttgtccttg ccagcacagg tgaaactttc cggcagaaaa
     2761 gctggtcggt atttgctgag gatgagtggc atctcacgga tgcacttgcg ctgactgcgg
     2821 gcagccgcta tgaacatcat gagcaattcg ggggacactt cagtccgcgt gcatatctgg
     2881 tctgggatgt ggcagatgcc tggacgctga aaggcggtgt gaccacggga tataaggcac
     2941 ccagaatggg gcagctacat aaagggatta gtggtgtatc cgggcaggga aaaacaaatc
     3001 tacttggtaa ccccaacctg aagccggaag agagcgtcag ttatgaggct ggggtgtatt
     3061 acgataaccc cgccggtctg aatgccaatg tcacaggttt tatgactgac ttctccaaca
     3121 agattgtctc ttattccata aatgataaca ccaatagcta tgtaaacagc ggaaaggccc
     3181 ggttgcatgg tgtggaattt gccggcacat tgccgctgtg gtcagaggat gtcacgctgt
     3241 cactgaatta cacctggacc cgaagtgaac aacgtgatgg tgataacaaa ggtgcgccgc
     3301 tgagttatac ccctgaacac atggtgaatg cgaaactgaa ctggcagatc accgaagagg
     3361 tggcatcatg gctgggtgcc cgttatcgcg ggaaaacacc acgtttcacc cagaattatt
     3421 cgtcactgag cgctgtacag aagaaagtgt atgatgagaa aggagaatac ctgaaagcct
     3481 ggacggtggt ggatgcaggt ctgtcgtgga agatgacgga tgccctgacg ctgaatgctg
     3541 cggtgaataa cctgctcaac aaggattaca gtgacgtgag cctgtacagt gccggtaaga
     3601 gtacgctgta tgccggtgat tacttccaga cgggatcatc aacaacagga tatgtgatac
     3661 ctgagcgaaa ttactggatg tcgctgaact atcagttctg ataataacaa aacgctatca
     3721 ctgacggtag aatacgttgc cactgcaact cctggcggaa cagtggcaac gtattaggtt
     3781 aagtgcattt ccgatccgct aatgagattt cgttaccaac aactaatatc gtcacaggaa
     3841 atgcacggat tatttttaac ttatcattta catacttgtc cagagtgtaa gcgcaccgtg
     3901 aaggacgtgg ggtaaaaatt agtttacaga gagagtgacg ttccagggca acaactcttt
     3961 catgcggttg gcaggccagg tgttgattac actgatcacg tggcgtagcc acgtttccgg
     4021 ctcgattccg ttaagttttg cagctaccga tcaggctgta catcactgcc gcactatcgc
     4081 tcgtcatctc aaagtccggt ctcgtcagca ggaaggtatc attctctccc gccatttttc
     4141 cgggggcccg gtcagataag tccctttgtc tatcgctgac tcctggctca taacccggtt
     4201 agcagaatgc agggtcacca cctcgccacg accgaatccg gataagtcaa ttgcaccttc
     4261 tcaatcgcca ttttgtcaca attaagtttg ataaagcagg tcacctctgc actgaatctg
     4321 ctccagtgcc tcattggtct ccgtccaccc ctttaaactg ccggataaga ctgctgcaca
     4381 gcgccttccc cggaatcaaa tcactttccc tgtccgccac aatccgcttc atgccacctc
     4441 gtattttgtt tcagagaccg gaatctgcca gaaagtgact acgcgtgcac atatcggttt
     4501 tctgggttac atgcatcgtg ttattttctg aaatgcaaaa taataaaaaa caaggacaca
     4561 tcatgaagga tgaaatacac agtaccagca ttaacccgga tgttgttgag cagtttctgg
     4621 cccttatgaa cacacattgt aaatcctcag aagaggcact cgccatgctg agtacgcttt
     4681 gttacaacct gatgatgaca tgcggtatcc attccatgga cagccagatg ctcaatgggc
     4741 atgggatatc catgcaactt caggggattg atattgccga aaggccaggc aagaatattc
     4801 actaacctga acagggcctg aaaacgctgt cttttcaggc ctctgcaggc aaaaactgat
     4861 gacaacaaaa tttctgcgtt tgtaccccac cgatgaaaaa gacaaaaact cacaccgggc
     4921 ttctagccag taaagataaa acccgacgtg tcagcctcta cgaaacgcca acggcatggt
     4981 gtatcagggg gcaggagtgc tacagcaaaa gtacaggacg ccgctgtggc agccacgaca
     5041 gtctgagcag actgcgcctg gacagcatta agcctgttga atgagtggtg gcaccatgaa
     5101 agataacaac acaaccgcca ccccagaagc ctggaaaatg cgttttctgg ccaacgggac
     5161 atccggcaca tggttttaca ctgaagacat tgcagtcact gacgcgtggc ttcgcgcgca
     5221 cccggatgat gtggaggtta tccctctctg ccggatacag ccccgccagt attcccatga
     5281 acaacatatt gaagaactcc gcgcagccct ggcccggatg ctgcatatgc atcatatgat
     5341 gctggaaacc acgcattaca gggaaaacat caccgatgcc cgttgtatcc gtgaaatgca
     5401 tgaagctcca ttacaggcgc tcaatattct ggccagatac ccgcataaaa aggacgaggt
     5461 gtgacgcatg tggctactga ttttctggct ttcatctccc tttaatgtct cttccggtga
     5521 tgcccggcag cccacactgg cggtgcacag ccaggcattt cataccgaac gggcctgtcg
     5581 cgatgcattg cgcgccatta aaaaggagaa tgttggcgaa ttttctctgc ggggcgtttg
     5641 tgtttctgac ggaaatacga aggaggaggc tgagagctca tgacggatat tactgacaga
     5701 catacacttc gccgtatgtc ctggtcggaa ctcttcacgg cagcacagga agcggaattt
     5761 cagcgtgatt acgagcgtgc cagaatagtg tggtcattcg ccttacatgt tgcaacaacc
     5821 acgattaaca aaaatctggc catagctcat attcgacgct gcgatacttt gttgcataaa
     5881 tcaaaaacag tacccggaaa caacacgggg ggccggtcag tgtgcctgag accccaacat
     5941 ccccggagat gactgacatt tccggactga cgcagttgtt gcatatctgt ggcaatacgt
     6001 tgtatccagc caagttgcgt tatttcgtcg gttgtttcgt gggcgtagtg atgtatgggg
     6061 ccttcgatgg gaaagtaaaa ctagcggaaa ataaaagtac tcgcctgcct acggaccttt
     6121 ttactcaggg tatttttgtt ttccataacg ttgcgtatta acatgaagga gaacgagggg
     6181 gattaggtaa tctctttcca tgaaaatacg caaacgttca tcaccgaagt gaaaaatcag
     6241 tttttgatat ttaaatgatt tttcaaatca tatttcagca ggacactacc agcacctaac
     6301 agcagcacat ctttgatgac aaaactgtca acttttccca gttgtggtaa cagacttagt
     6361 gtgactaaac ctgtaacaat aacgataata tctcccagta tgccagcagc aggcctgaag
     6421 aagccgatac tcagtgctag aaaagtgata gtttccacta tgccgaggaa atagctcccc
     6481 ccatgaacac caaacataat gtacaggata ttcagccacg tgggagaaat caggggcttg
     6541 agggccatca cttcaaaatc aaaccattta taggtcccga agagcataaa tattataata
     6601 acagacaggc gaagaataac tatgtcataa tctgatttaa tgtaaacatt aataaatttt
     6661 ttcatttcat tctccgggtg gaaaattata tgcactaaaa aggattaagt acgggtatca
     6721 agcattttaa ttaaagcgtt aacggttcct gaataggtat catgtggtgg aagtcgattt
     6781 aacatcagtg ccccctttag tgtagaacag actataacgg cgagctcatc cggaagaaga
     6841 actgttttga actctccact attctgcccg gaggtgataa tatctttaat catctgtatc
     6901 tcctgatgtg ccagttgact tacagcctcc tgtaattcag gagaaaactg atggctgtcg
     6961 gatagcatgg cataaatacc gcacatttcc cctctttcag cacaaccagc aaatgcatca
     7021 agatatgtcc tgagacgttg tacaccagaa gaaacattat ggagagttat attaagtcta
     7081 tcaaatactt ctgttttgta ttggcagtag gctataccaa gatcagtttt cctcgggaaa
     7141 tggtgatgaa tactggcttt tctgatacca atttcttttt ccagatctgc gtaactgaac
     7201 cccaaaaatc cgcgttgctg gatcagagta tccgttatgc gaagtatatt ctcataagta
     7261 gacacggctt tacctgttat gtattaaagg gactattata catattttaa cgatagataa
     7321 tggcagttcc atcaagttta ttttttcctc ccaatgaagt gattcggaaa tgagaacctc
     7381 ctgcctgctc cgctttgtct ttaagcgcag ttgccacctc ttctggtgta tatagattac
     7441 caacagaaat atagtctgac ggcatcaaac caacaacgtc ctgacgatta atttcatctg
     7501 cagcagttgc gtagcctgtc agaatgagac caagggcgac aatggtagtg cagataacat
     7561 aattcatgag cagtttctct ttatgtttgc gatactacac atgctacctt ctgattggta
     7621 ggcttgcaag atggatttat ttaaaataag agggatgatt tgtatcaaat tgttttgtat
     7681 ttttgtgttt tgtatcactg aggtgaagtt gtaagatctg gccctctgcg aggttggctc
     7741 tgtcgaaagg aacaggtgac gagagttaac atcgttgcct tggctgcatg ggggatatgt
     7801 agctaccccc tgatatttcc gccacggaag ccaatatgaa caacaataac aatacgcctc
     7861 gtttccagta tgcttcatgt cggatatttt gtaagcgcta tgtacttccc gggacgctta
     7921 tcttttagcc gtagctggta gtgcaataaa agcctggcag caatccaggc ctctacgttc
     7981 taagaatcaa ctgtcagtgc agaggaatgc gcacgacgct ctcgttgtta ttggcttttg
     8041 ccgtcgcacc gtcgtttttc acggtgacaa acagcgcgtg cgctttgcta tccgtcttat
     8101 atcactggtg ctgacaatgt ggatgaggtc gcctttaaaa aaggccatcg ttatataacg
     8161 gtgatctccg atcgcgatgg tcgtgcgctg gccttaacgg acgattgtgg cacagagagc
     8221 ctcgccggct atcttcgcac gcttactgac gagcagttgc tggctatcaa aacgctctca
     8281 atggacatga atgcaggcta tataagagca gcgcgtatcc acttaccctg tgcggttgag
     8341 aaaatcgcct tcgaccgctt ccatgtagca aagcaacttg gcgaagtggt tgataaaacc
     8401 cgtcagaatg aacatccgca cctccctgtt gaaagttggc gtcaggcaaa gggggcccgc
     8461 ttcctgtggc agtacagcga taaatggatg accaaatcca ggcaggaaaa gctgatatgg
     8521 ctgcgtgcac agatgaagct gacaagccag tgctgggcgc tgaaagagct ggcaaaggat
     8581 atctggaaca ggccatggag tgaggaaaga cgtaatgact gggagagatg gctggctctg
     8641 gcggccaaca gtgacgttcc tatgatgaaa aacgccgcga aaacgatagg aaaaaggctt
     8701 tacgggatcc tgaatgccat gtgtctcaaa cggaaacgcg gaggcgctta acagtaagat
     8761 caggctactg aggataaaag ccaggggata ccgaaatcgg gagcgcttta agctgggcgt
     8821 gatgttccac tacgggaaac tgaatatggc gttctgagcc ttcccatcat gatcagggaa
     8881 aacccatgta tcatcaggtt attatgtgag ctggtaaatc agattttgca tggactgaaa
     8941 tcatgctgtt atttagggat aaagaaccgg ctttttgtcc cgccccctcg ccattaatga
     9001 gaaaaagaat ggaatgctcc ctctgaggag aagggcagta gaaaaaagaa cccgccagca
     9061 aaaatattgg ggatgaacag ctttcgctac tcagattgct ggcgggtata gttcctcatg
     9121 aaataaaaat actacgtagt ctttttaatg caaatgataa tcattgtcaa ttgtttgtgc
     9181 gtgttttctt catacatgat tggtagtaaa ggtgattcag gctatcagtt tttgctgatg
     9241 atctttttat ctatagaaaa catcagccaa gctggttggt tcagtaacgc tttcatcttt
     9301 gagcatttta taaaaaattc ttgaattgtt aaaatagatt aaagtctggt aacagcaaaa
     9361 gttaaacgag aaatcaaaag gaggtcccaa atctgaaaat gaagcatctg cgtgatgatg
     9421 ttgcagcggg acgtcgtcgg ctgtatatca gagcaatctg tccatccctg cgtgaagccc
     9481 cgttacttta ttcgtacccc ttataatggg gtgttagcca gccagacccg gcatgattac
     9541 tgtgaaccgc cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga
     9601 ctaaaaatac acgtttttcc cccgaagtcc gtcaacgggc agttcgtatg gttctggaaa
     9661 gtcaggacga atatgactca caaagggcgg caatttgttc cattgctcca aagactggct
     9721 gtacgccaga gactctgcgt gtctggattc gccagcatga gcgggatacc gggggcggtg
     9781 atgg
//
LOCUS       DAPOZO010000143         9741 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPOZO010000143 DAPOZO010000000
VERSION     DAPOZO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.143"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(13..2256)
                     /locus_tag="ROI21_004314"
     CDS             complement(13..2256)
                     /locus_tag="ROI21_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDX5113159.1"
                     /translation="MNGDNSVETRGAYSAGLLSQVNDSGIVENNTRLETTDKTNIVTY
                     GENAVGVLACSSPGESRTCVDAVDDEVSDSNSYEVISRADLKMNGGSITTNGTNSYGA
                     YANGEKAYINLDYVALETGEHGSYAVAIRQGNIDIKNSSITTKGTKAPIAKIYNGGEL
                     FFSNVTAVSEQDKGISIDASNIDSQAKIALSSVELSSALDSIDVNKTTTDVSILNRSI
                     ITPGNNILVNNTGGGLNIISSDSTLNGATKLVSGTTTLKLSENTIWNMKDDSVVTHLT
                     NSDSIINLSYDDGQTFTQGKTLTVKGNYVGNNGQLNIRTVLGDDKSATDRLIVEGNTS
                     GSTTVYVKNAGGSGAATLNGIELITVNGDESPADAFRQGDARIAAGAFEYQLKQQGKN
                     WYLTSYQSVNEEDNSSEGNSESTETPTPVLRPEAGSYVANLAAANTLFVMRLNDRAGE
                     TRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSHRYVSQLGADLLTGGFTDSD
                     SWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYSAGLYATWFADDISKKGAYIDAWAQ
                     YSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKYTWIFQPQAQAIW
                     MGVDHNAHTEANGSRIENDANNNIQTRLGFRSFIRTQEKNSGPHGDDFEPFVEMNWIH
                     NSKDFAVSMNGVKVEQDGARNLGEIKLGVNGNLNPAASVWGNVGVQLGDNGYNDTAVM
                     VGLKYKF"
     gene            complement(3076..3294)
                     /locus_tag="ROI21_004315"
     CDS             complement(3076..3294)
                     /locus_tag="ROI21_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113160.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(3426..4949)
                     /locus_tag="ROI21_004316"
     CDS             complement(3426..4949)
                     /locus_tag="ROI21_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDX5113161.1"
                     /translation="MRNTLIPIFIAICLFITGVTILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSRLIVPLTRSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(5285..5533)
                     /locus_tag="ROI21_004317"
     CDS             complement(5285..5533)
                     /locus_tag="ROI21_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113162.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVLMTHGYVDSHIIDKALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(5646..5912)
                     /gene="ariR"
                     /locus_tag="ROI21_004318"
     CDS             complement(5646..5912)
                     /gene="ariR"
                     /locus_tag="ROI21_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HDX5113163.1"
                     /translation="MLEDTTIHNAITDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(5941..6213)
                     /locus_tag="ROI21_004319"
     CDS             complement(5941..6213)
                     /locus_tag="ROI21_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDX5113164.1"
                     /translation="MKTSDNESTKYEITGQAVLHILRMKINFSLQTLIKQLLVMKSVE
                     ENAFRRDLIDSIIRDFSNSDTGEPNRRTAIADNKSMFNGKKINRIH"
     gene            complement(6256..6492)
                     /locus_tag="ROI21_004320"
     CDS             complement(6256..6492)
                     /locus_tag="ROI21_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDX5113165.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            6806..8017
                     /gene="bluF"
                     /locus_tag="ROI21_004321"
     CDS             6806..8017
                     /gene="bluF"
                     /locus_tag="ROI21_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDX5113166.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQAAYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALDLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
     gene            8222..8953
                     /gene="bluR"
                     /locus_tag="ROI21_004322"
     CDS             8222..8953
                     /gene="bluR"
                     /locus_tag="ROI21_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDX5113167.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIEAPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            9174..9578
                     /locus_tag="ROI21_004323"
     CDS             9174..9578
                     /locus_tag="ROI21_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDX5113168.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHYRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            9631..9741
                     /locus_tag="ROI21_004324"
     CDS             9631..9741
                     /locus_tag="ROI21_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113169.1"
                     /translation="MEIKVQRLFIWMSNTIFLLPPINNHQINTINLIFEM"
BASE COUNT         2847 a         2131 c         1907 g         2856 t
ORIGIN      
        1 agctaacggg gatcagaact tatatttcag gcccaccatc actgcggtgt cattgtagcc
       61 attatcaccc agctgcacgc ccacattacc ccagacgctg gccgccggat tcagattgcc
      121 atttacgcca agtttaattt cccccagatt acgcgccccg tcttgttcga ctttcacacc
      181 gttcattgag acagcaaaat ctttactgtt atggatccag ttcatttcaa caaaaggttc
      241 aaagtcgtca ccgtgcggac cgctgttttt ctcctgagta cgaataaagg agcggaaacc
      301 aagtcgggtc tggatgttgt tatttgcgtc attctcaata cgtgagccat tggcttccgt
      361 gtgcgcatta tgatcgacgc ccatccagat agcctgtgcc tgtggctgga agatccacgt
      421 atatttcgcc gcttccagac caaaggattt attcagggca aaaccgtaac ccgcttccag
      481 cgagacggtt gcacctttcg cgctatagga ttcataggct aattcatctc cttttaccga
      541 gtttttaaac cagctatatt gcgcccaggc gtcaatgtat gcgccttttt tactgatgtc
      601 atcggcaaac caggtggcat acagccctgc gctatagcct ctgacactcc ctttcgaacg
      661 ataatccgac acgctggaat gagttgagtt gtagtcgcgg gcataaccag ccatcactcc
      721 caaacgccaa ctgtcactgt cggtaaaacc accggttaac agatccgccc ctaactgcga
      781 gacgtagcga tgcgaggtcg ttctcaactg tccgttgctg tcacgccagg cattatgccc
      841 gccaatttga cgtagccaaa gtcggcttga acgctcctgt tcagttacag gatcgatgta
      901 gcgcgtttca cctgcacgat cgttcagacg cataacaaaa agagtgttag cggctgccag
      961 gttggcgacg taacttccag cttcggggcg cagaacgggg gtaggcgttt ctgttgattc
     1021 tgaatttccc tctgagctgt tatcctcctc attcactgac tgataacttg tcaaatacca
     1081 gtttttgcct tgttgtttta gttgatattc gaaagctcca gctgcaatcc ttgcatcacc
     1141 ttgtctgaag gcatctgctg gagattcatc gccattcaca gttatgagtt cgatcccatt
     1201 tagcgtggcc gcgccgcttc ctccagcatt tttcacatag acggtagttg aacccgaagt
     1261 attaccctca acaataagtc tgtcagtagc cgatttatca tcacctaata cggtgcggat
     1321 attaagctga ccattattac cgacataatt accttttacg gttaacgttt ttccctgggt
     1381 aaatgtttga ccatcatcat acgaaagatt gataatactg tctgaattag tcagatgggt
     1441 aacaacggag tcatctttca tattccagat tgtgttttct gaaagcttca gtgtagttgt
     1501 gccgctaacg agtttagtcg ctccatttag agtagagtcg gacgaaatta tgtttaagcc
     1561 acctccagta ttattaacca gaatattatt accaggcgtg ataatacttc gattaaggat
     1621 gcttacatcc gttgtagttt tgttaacatc aatactatcc aaagcacttg aaagttcgac
     1681 acttgatagt gctattttgg cttgagaatc gatatttgat gcatcaattg atattccttt
     1741 atcttgttct gataccgcgg tgacattgga aaaaaataac tctccaccat tgtatatttt
     1801 tgcaatgggg gctttagtgc cttttgttgt aatagaacta ttttttatat caatgttacc
     1861 ttgtcgaata gcaactgcat aacttccatg ctccccggtt tcaagtgcca cataatctaa
     1921 attaatatat gctttttccc cattagcata agcaccatag ctattagtgc cattagttgt
     1981 tatggaacca ccattcattt ttaaatcagc acggctaata acttcgtaac tgttagaatc
     2041 actaacttca tcatctacag catcgacaca tgttcgagac tctccaggac ttgaacatgc
     2101 aagaacacct actgcatttt ccccataggt aacaatgttc gttttatctg tggtttcaag
     2161 acgggtgtta ttttccacaa tcccagaatc attaacctga cttaaaagtc ccgctgaata
     2221 tgcccccctc gtctcgactg aattatcacc attcataacg atggtgccac caaattcact
     2281 gactactgcg ctggctgtta tccctccatt aacatcttgg ttatttcctt tgtcagatac
     2341 gcttccagag gtcttaatat cgctatcgct tatatataat actttgttat gactatcaac
     2401 agaaatacca taagatccat caccactagt catcacactg gcattattta tatatccagt
     2461 tacaccgtca tccactttca cagcatggct ttcgtcttta gtggttagat actctccatt
     2521 acttatcgaa ttattatctt tgatttcaat cggcgattct tcatcagtta tataggaaag
     2581 tatactataa tcatccaaca attgtgttgc atatgcatta cccaccgcca aagcaataag
     2641 tcctaaacta aacccgggga gttttttcag tttcatagat taaccatcca ttcattggat
     2701 taacaaacat tcctggtcaa ataacctatg agttcagatt accaaaacgg agggtaaaga
     2761 caaaaacaaa agcacaaaaa atttaaatag ataaacagcg acaatatata aatgataaat
     2821 aaggcggcat tatcattttt caccttattt tcatgacatt gatcactttg gggtgacgat
     2881 tttgtttaac aaataactct tcgacatcaa aattattaat agttgccata gaaattattt
     2941 cgatgatata taaaaccaac tgtgataaca ccagggttga caactatttg cgcaataatt
     3001 cctttccttc aatgatccag gcgttcactg tcagcacccc attcagccgc atactgacag
     3061 tgagaaactg aaagattatt gctgatccct gtggctgacg agtctttccc ttaatcgatt
     3121 aaccagattg atcgccagcc aaacaagggc attaagcaaa tttatcgtca caagcacttc
     3181 tcccagcccg gtactgggtt ttgcaataag gatgaccagg agagtgatca gcataaatgc
     3241 aatggaaaag atgatataga gagtgaaata atccagatta ttactattgt tcatgctact
     3301 aatggcaatc ctgtcaaaac tttcaaacaa ttatagcaaa caaaataatg aacaatagta
     3361 acaagaggca acgagctaat tagctgtcta taattacacc gcaccatggc cttactgatc
     3421 gattttcact caaccacaac cttcaccttc ggtttagaaa ggataatttt gaccaggtcg
     3481 atgtatgtta ccggtttata gaaataataa ccctgctgaa atgtgatatt attttgattc
     3541 agatagtcaa gttgttcttt cgtttcgaca ccttcagcga cgatactgat cgaaagttta
     3601 cgcgccagat ccaatacaca atccagaatc cgagttgatt ctgggtctgc gtttacgcgg
     3661 ccaacgaaac tgtgatcaat tttgatataa tcaatatgca ggtcatgcag ataagaaagc
     3721 cctgagtagc cagtaccgaa atcatccagc gcaatgacaa aaccattttc atgcagaatg
     3781 ttcaaccgct gaaccagact ttcatcaaca tttaatggct cacgctcggt aacttccaga
     3841 acgaggttta aatcgcgacg ggtaaaactg tcacggtaat ttaagcactc gtcgacaaat
     3901 gtcggcgaaa taatatgcga ggcgctaaaa ttaatcccaa tatgaaaacc ttccggcagt
     3961 ttactcgcga tagcgttcat ctgtctggca acctgattca tcaggcttcg cgtaagcgga
     4021 acgattaatc tggatttttc agcaagtgga ataaatgccg cgggtgatat atatccaccg
     4081 tgaggttgtt tccagcgggc taacacctca actccccgca atgtcccttc ccgaccattt
     4141 accacaggtt ggtaaaatgg gacgatctcc ccattattta tagcccgtcg caggatctct
     4201 tcaggcgttg cgcttttatt gaaataccta tcaagcagga aggcagcggc gcaggcgatt
     4261 aacagaataa atatcaaaat cccaaagcct ttatcgataa gtctgtagaa gctaaagagt
     4321 ggcggcgggt taaagataat agtgaaatga tattttgtgg agtgaacctt ctcggtatag
     4381 ggatagtgtc cattaagcgt catcacatca cccgtaggtc caataatgtc atctgccacg
     4441 cgtaatacat accttacccc tttcaagggt acattaagcg ccccgcgaat atgctgatca
     4501 cttatcgtaa ccaaaatgcg cgtatctgca aattggctct gatagagcag gataggtaat
     4561 ctattaacgg tgtcgattgc cggagccagc agtaaattac tgtccgggaa aacaggaata
     4621 cgagacagta ggacccgatt ccccggtaag gatgtacacc agaccgttcc ctgtttgaga
     4681 atgatgattg tgcgcaggtg aggtttcaga gctgcttcag tgccaagctg atattgctcc
     4741 tcgaggtcgc attccttccc ggcaatgtta accgccgttt gagtcgcctg tgacgcttct
     4801 tcaagtatat gattgatatt gttggcggca tatcttgctc ccgccaggta ctctgctttt
     4861 gcggaatacc agagctggat gttcaaaata gtgacaccgg ttataaacaa gcatatcgca
     4921 atgaagatgg gtatgagtgt attgcgcatt tgcttgtccc ggccaaaggg tttaatacat
     4981 gaaaatgcca ttcattcgct cgcactggca tctacagaga gcatggtaga gagcataaga
     5041 aatatccacg ctgaagataa aattttctcc atcttcatct aacctattaa ttattgagca
     5101 caatgtagtc agaattaaac aaattaggtg ggatatcagg cgtcaagaat ggaaaacgag
     5161 cgctctccat tcctgtttgc cccctgatat tgcggatata ttaagaaagc ataacgcctg
     5221 aaatgctcac tttgcaccag catgatgata cagctgatgt ttattctata accttactca
     5281 agttctaaga gagcactgat tccctgtcat taataatgat atccgttagt gtagtcttat
     5341 gcatttcaat gtactgcaag atttgcagat agagatcaga aagaatcaca ctagtttcat
     5401 cttttaaacg caagcgtaaa gccttatcaa taatatgact atcaacatac ccgtgcgtca
     5461 tgagcacctc cctctcgaga tttttaaaca aagcacattg aaaaataaat ctctcgggga
     5521 ttgaattatt catgtcagcc tcgtaggaat attgacccaa taaaattatg ctgtgtatag
     5581 tctaaagcga cagacaaaaa taagatgact gagttttcta taaacttata cttaataatt
     5641 aaaagttaca tatcatcagc tgtgtatcgc aacacgattt ccagtgtttt tctaatcaca
     5701 tcagctttga gaatatcact tgttgtttcc agagagtgta tcagacttaa gataatattt
     5761 ttattattaa cattatcgcc tgaaagcatt aaattggtca cagcctgccc taacacagct
     5821 gactcttctt ctaacaaatt acctgaacta cgaaagtagc ttgataacgc tttatcagtt
     5881 attgcattat gaattgtagt atcttcaagc atggtaacct ctcatcttac ttatgaaatt
     5941 ttaatgtatt ctgtttattt tcttaccatt gaacatactt ttattgtcag ctatcgctgt
     6001 tcttctgttt ggctctcctg tatcactgtt gctaaaatca cgaattatgc tgtcaattaa
     6061 atctcgtcgg aaagcatttt cctcaaccga tttcattacg agtaattgct taataagagt
     6121 ctgtaacgaa aaatttatct tcatgcgcag tatatggagc accgcctggc cagtaatttc
     6181 atattttgta ctttcattat cagatgtctt cataacgact tcatagctaa cctgttaaat
     6241 aatcaggctg ttgcattatt caaaaagcaa cccaattagt gcattataat gtttctgttc
     6301 ctcttcccct gtcgcttgtt ccagtcggca tagaagtttg gcacaaagcg actttcgact
     6361 cagattacgt ccatctttca aaatctcagt cacaatttcc ccgagagttt cctgctgagt
     6421 aggcaagtta gcttttgcaa agtaacgagt gattgctccc gcagaatcta aagtcactga
     6481 attttgatgc atgctacgcc tctgttaaaa atgttaatca atgttaagtg aggtaacaat
     6541 agcaaacttg tacgaaatat gtgtacagaa tgataaatta tttttttgca tttagtgcta
     6601 atacatataa aacaacaggt tatgataaaa aatattgcat catttaaatg acaacctata
     6661 caagaatttt cgccaactca tcttcaactt tttccatttt ttatcaatgt aaagaaacag
     6721 ttatgttttg gtattaaaag aaagacaccg gaagatatgt ctgttaccgt cttactctcg
     6781 cctctcccat taccctggac agttaatgct taccaccctt atttatcgta gccatatacg
     6841 tgacgacgaa cctgtcaaaa aaatcgaaga aatggtttcg atagcaaatc gcaggaacat
     6901 gcagtctgac gtaacaggga tcttactgtt taatggatct cattttttcc agcttctgga
     6961 aggtccggaa gaacaggtta aaatgatata tcgggctata tgccaggatc cacggcacta
     7021 taatattgtt gagctgctgt gcgattacgc gcctgctcgc cgttttggca aagcgggaat
     7081 ggaattattt gatttgcgcc tgcacgagcg agatgacgtt ttacaggccg tattcgacaa
     7141 aggcacatca aaatttcagc taacttatga tgacagagcg ctacaatttt ttcgtacttt
     7201 tgtccttgca accgaacaag cagcctattt cgagatccct gccgaagact cctggctttt
     7261 tatcgctgac ggatctgata aagaacttga ttcctgtacc ctttcaccaa ctataaacga
     7321 ccactttgcc tttcatccta ttgtcgatcc cttatcgcgg cggataatcg cttttgaagc
     7381 cattgtgcaa aaaaatgaag acagcccgtc agctatagcg gttgggcagc gtaaagaagg
     7441 ggaaatctat acagcggatc tcaaaagtaa ggcgcttgca ttcgcgatgg cacacgcact
     7501 tgacctcggt gataaaatga tttcaatcaa tctattacct atgaccctgg ttaacgaacc
     7561 tgacgcagtc tcttttttac ttgatgaaat aaaggccaat gctctggtgc ctgaacaaat
     7621 catcgttgaa tttactgaaa gtgaagtcat atctcggttt gatgagtttg cagaagcgat
     7681 taaatcgctt aaggctgccg gtatcagtgt agcaattgat cattttggcg caggttttgc
     7741 cggtttgtta ctcctgtcac gcttccagcc tgacagaatt aaaatcagtc aggaattgat
     7801 taccaatgtt cataaaagcg ggccacggca ggcaattatt caagcgatca taaaatgctg
     7861 tacatcactt gaaattcaag tcagtgctat gggcgtggca acaccagaag agtggatgtg
     7921 gcttgaatcc gcaggaattg agatgtttca gggagatctg tttgcgaaag ctaaattgaa
     7981 tggtatccct tcagttgcgt ggccagagaa aaaataattt tcagcatatt atttcacatg
     8041 atttcagtaa atcatcgtga aaatgaatac gttatgttta aatttaatcc acattaaaaa
     8101 taatgtttca ttcaatttag ctgaaatctt atttatactt tgtacaattt tagcgtatat
     8161 tgtacaaagt attattgggt cgtgtacagg cgacggagat ttgtgaccgc aaggaggaat
     8221 tgtggcttat tacagcattg gtgatgttgc tgaacgttgc gggattaatc ctgtcactct
     8281 ccgggcctgg caacgccgct acggtttgtt aaaaccacag cgcagtgaag gcggacaccg
     8341 actctttgat gaagaagaca tacaacgcat cgaagagatc aagcgttgga taagtaatgg
     8401 cgtccctgta ggcaaagtta aagcattact ggaaaccacc agccaggata cggaagatga
     8461 ctggagccgc ctgcaagaag agatgatgtc aattcttcgt atggctaatc ctgccaaact
     8521 acgcgcgaga attatttcac tgggtcgaga gtacccagta gatcaattga ttaatcatgt
     8581 ttaccttcct gttcgccagc gtctcgtgct tgatcacaac acctcccgca ttatgagcag
     8641 tatgtttgac ggcgcattaa ttgaatacgc agcagcctcg cttttcgaaa tgcgccgtaa
     8701 gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa gagagagcac gattgtggct
     8761 ggaagcatgg cgtttatcat tgtcaggatg gcacatttct gttcttgctg atcccattga
     8821 agcgccgcgc ccggaactgt tcccaacgca aacattgatt gtctggacag gcatggcacc
     8881 aacgagaagg caaaacgaac ttttacaaca ctggggtgag caaggttata aagtcatctt
     8941 tcatgccccc taatttcacc tttatcccgc cccttagaca gaatcttata atctgaaccc
     9001 acaacagcgt ttgtaccgga gctggtccgt tgcaaaatgc tcaccgggag aattcaggta
     9061 atcccccttt cacactttgt acatatcaca gccaaaggac agaccagatt gaagtggtaa
     9121 aaccgatcgt gcacactacg cagtctcaca actaacaaga ggttattatc ctgatggatc
     9181 aggttgttgt ttttcaaaag atgtttgagc aagtgagaaa agagcaaaac ttctcctggt
     9241 tttattcaga attaaaacat taccgtattg cacattacat ttattatctg gctacggata
     9301 acatcagaat tattactcac gatgacacgg ttttgttatt aagaggaacc aggaacctgt
     9361 taaaagttag tacgaccaag aaccctgcta aaataaaaga ggccgcattg cttcatattt
     9421 gcggaaaatc aacatttcgg gaatactgtt caacactggc agacgcgggc gttttccggt
     9481 gggttactga tgttaatcat aacaaacgca gttactacgc cattgataat acgcttttat
     9541 atattgaaga tgtagaaaat aataaaccat taatctagct aaagttggat gcttaaaaaa
     9601 tgcttcataa ttcagtaagg cattagcata atggaaataa aagtgcagag actattcata
     9661 tggatgagta atactatctt tttattgcca cccataaata atcaccagat caatactatc
     9721 aacttgatat ttgaaatgtg a
//
LOCUS       DAPOZO010000144         9536 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPOZO010000144 DAPOZO010000000
VERSION     DAPOZO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.144"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(31..177)
                     /gene="ecnB"
                     /locus_tag="ROI21_004325"
     CDS             complement(31..177)
                     /gene="ecnB"
                     /locus_tag="ROI21_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDX5113170.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(288..413)
                     /gene="ecnA"
                     /locus_tag="ROI21_004326"
     CDS             complement(288..413)
                     /gene="ecnA"
                     /locus_tag="ROI21_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDX5113171.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(465..1031)
                     /gene="efp"
                     /locus_tag="ROI21_004327"
     CDS             complement(465..1031)
                     /gene="efp"
                     /locus_tag="ROI21_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDX5113172.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1073..2101
                     /gene="epmB"
                     /locus_tag="ROI21_004328"
     CDS             1073..2101
                     /gene="epmB"
                     /locus_tag="ROI21_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDX5113173.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     CSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            2336..3205
                     /locus_tag="ROI21_004329"
     CDS             2336..3205
                     /locus_tag="ROI21_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDX5113174.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDSSKCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(3255..3608)
                     /locus_tag="ROI21_004330"
     CDS             complement(3255..3608)
                     /locus_tag="ROI21_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDX5113175.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(3746..5392)
                     /gene="groL"
                     /locus_tag="ROI21_004331"
     CDS             complement(3746..5392)
                     /gene="groL"
                     /locus_tag="ROI21_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDX5113176.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(5436..5729)
                     /locus_tag="ROI21_004332"
     CDS             complement(5436..5729)
                     /locus_tag="ROI21_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDX5113177.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            6005..7261
                     /gene="yjeH"
                     /locus_tag="ROI21_004333"
     CDS             6005..7261
                     /gene="yjeH"
                     /locus_tag="ROI21_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDX5113178.1"
                     /translation="MSRLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(7277..7753)
                     /gene="fxsA"
                     /locus_tag="ROI21_004334"
     CDS             complement(7277..7753)
                     /gene="fxsA"
                     /locus_tag="ROI21_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDX5113179.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            8090..9526
                     /gene="aspA"
                     /locus_tag="ROI21_004335"
     CDS             8090..9526
                     /gene="aspA"
                     /locus_tag="ROI21_004335"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDX5113180.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
BASE COUNT         2394 a         2414 c         2293 g         2435 t
ORIGIN      
        1 ggcacgacat aactgtcgta ccgttattgc ttactgctgc gctttcgttg ctgcgccaga
       61 aatcgcgtta ccgccatcag aaatgtcttc accaacgcca cgcgtggtgt tgcaggcagt
      121 taatactgtt gaaagcacca gaacagaaaa gatcgctgca attgtcttct tcaccataac
      181 gtcttccttt taggtaatgt tatttatgtt tgcctatagc aaattaataa tagacaacat
      241 caccaaaaat gatggaaatc tgatgatttt tcggaagaga agaaaaatta gctggcagcg
      301 cgggagatgg aattaccgag atgtttaatg tcttcaccaa aaccgcgagc ggtgttacag
      361 cccgtgagca gcgcactggc aagcaaaaca agaacgataa ggcgtttcat catctctgac
      421 ctgaataagt gatggtgcag cctgcaggcc gcaccacaac cgcattactt cacgcgagag
      481 acgtattcac cagagcgggt atccactttg atgacttcgc cgatttgtac aaacagcgga
      541 actttaacca cagcgccagt agacagggta gccggtttgc cgccagtacc tgcggtatca
      601 cctttcaggc ccggatcggt atccacgatt tccagttcaa cgaagttcgg cggagtaacg
      661 gagatcggct gaccattcca cagagttacg atacactctg cctgatccag caaccatttg
      721 gcgttgtcgc caattgcttt tgcatcagca gacagctgct cgaaagtttc gttgttcatg
      781 aagtgccaga actcaccgtc gttgtacagg taagtcaggt tcatatcgac aacatcagca
      841 ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc gagtaccggt cagcagacga
      901 cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt ttacgaattc actcgcttca
      961 accgcgtaag gttcgccgtc taacatgatt ttaagaccag cacgaaaatc gttgctatag
     1021 tacgttgcca taaggccctc tgaaatttgt taattggtag ctaagccaca aaatggcgca
     1081 tattgtaacc ctaaataccc catccagaga agattggtta acgcaacttg ccgatgttgt
     1141 gaccgatcct gatgaacttc tgcgtctttt gaatatagac gcggacgaaa aactgttggc
     1201 cggatgcagc gccaaaaagc tgtttgccct gcgtgtgccc cgctcattta tcgatcgcat
     1261 ggagaaaggc aatccgaacg atccgctttt gcgtcaggta attacctcgc aagatgagtt
     1321 tgtcgtcgcg cccggattct ccaccgaccc gctggaagaa cagcacagcg tagtgccagg
     1381 tttgttgcat aaataccaca accgggcgct tttgctggtc aaaggcggct gcgcggtaaa
     1441 ttgccgctat tgcttccgtc gccacttccc ctatgccgaa aatcagggca acaagcgtaa
     1501 ctggcagact gcacttgagt atgttgctgc gcatccggaa ctggacgaga tgattttctc
     1561 cggcggcgat ccgctgatgg cgaaagatca cgagctggac tggttgctca cacaactgga
     1621 agccatcccg catatcaaac gtctgcggat tcacagccgt ctgccgattg tgatcccggc
     1681 acgcatcaca gatgcgctgg ttgaacgctt ttcacattct acgctgcaaa tcttgctggt
     1741 gaatcacatc aaccatgcca atgagataga tgaaacattc cgtcaggcga tggctaagtt
     1801 gcgccgggta ggcgttactt tgctcaacca gagcgttctg ttacgtggcg tgaacgataa
     1861 cgcacaaacg ctggcaaacc tgagtaatgc gttgttcgat gccggcgtga tgccgtatta
     1921 cctgcatgtg ctcgataaag tacagggcgc ggcgcatttt atggtgagtg atgacgaagc
     1981 acggcagatt atgcgtgagt tgctgacact ggtgtcggga tatctggtgc ctaaactggc
     2041 gcgagaaatt ggcggcgaac ccagcaaaac gccgctggat ctccagctac gccagcagta
     2101 actgattaaa cagggaagtt taatctttgc acgttgcata acctttaatt attcatatac
     2161 attgctgtta ttacttttta tataaccaac accaataatt aacctttctt ttctgtttat
     2221 atagagtgag cacggctatt tatgttattt taacaactat tactaccaag cccatttatc
     2281 tggcttataa attaaaatta attattgaat tgagaaatgg ttagggagaa cctacatggc
     2341 cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt tcattgcact
     2401 ggcgctgaaa attaaagagc ctcgcaataa agaatcgttg ttctttctgt ctgtcatgga
     2461 actgcgcgac cttctgattg cgctggaaag ccgtctgcat caaaagcata aactggatgc
     2521 tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa tggcagaaaa
     2581 tataccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc gggttaacac
     2641 gctggaactt accgataatc agggtgaaaa tcttaccttc gtattaacgc tacatgacag
     2701 tagcaaatgt gagttagtca tcaatgaatt gcagattgaa atgctggcgc gagcaatcat
     2761 tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattactt cactgttaga
     2821 tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat atgacaccta
     2881 ttcccaaccg gaatggaaac ataacttgtt caaccattat ctggcggtgc tttaccgctt
     2941 taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc gtgaagcaac
     3001 gccgggtaaa gaagtggagg cgatcactcg ccgaatgctt gatttcagcc cacgactgaa
     3061 aaaactcgcg ggtgtaccat gtcaggtcta tgttcgcacc gtagcggcaa ataacgccca
     3121 accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgttc aatccaccag
     3181 caaaactgca ccacaggcga aataatccca tctgttttac tacgaggctg gaagtatcca
     3241 gcctctggat cccatcagtt cgggcactta tatacctggc cgataatctg gctatccgtc
     3301 gggacaaaac tggacaacat cccctgcgac gggctgctga tgccataaat cacgttaccg
     3361 cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc cgcgcataga accgccctct
     3421 tcaccgtgtt gcccggaaag ccagttgctt tgcttacctg tcgcagtacc aatcagctgg
     3481 cactctgcgc ctggctgctc gtccacaatg cgtacactct gaccggcagc actcaattcg
     3541 ttgctggagc tacaacccgc catcagtagc gcggcaccga caatccctgc taagtatttt
     3601 acgtgcatgt tattccccat aatcaatgag ctggacgcca ctcgcgtcgt ccgtgtctga
     3661 atcttatact agaaagatga ccaaaagaaa aacccccaga catttctgcc cgggggtttg
     3721 tttatttctg cgaggtgcag ggcaattaca tcatgccgcc catgccaccc atgccgccca
     3781 taccgccagc agcgcctaag tcagctgcat cgtttttcgg caggtcggta accatgcact
     3841 cggtggtgat catcaggcca gccacagaag ccgcgtactg cagagcagaa cgggttactt
     3901 tggttggatc caggataccc atgtcgatca tgttgccgta ttcttcggtt gctgcgttgt
     3961 aaccgtagtt accgtcgccg cctttaacgg tgttagcaac aacagacggt tcttcgccgc
     4021 agttcaggac gatctgacgc agcggagctt ccattgcacg cagtgcaact ttgataccca
     4081 cgttctggtc ttcgttctga ccacgcaggt cagccagttt agacgctacg cggatcagcg
     4141 caacaccacc accagcaacc acgccttctt ctaccgcagc acgggtcgcg tgcagggcgt
     4201 cttcaacgcg tgcttttttc tctttcattt caacttcggt agcagcacct actttgataa
     4261 ctgcaacgcc gcctgccagt ttcgctacgc gctcctgcag tttttcacgg tcgtagtcag
     4321 aagttgcttc ttcaatctgc tgacggatct gagcaacacg gccctggatt gcagcttctt
     4381 cacccacgcc atcgatgatg gtggtagtgt ctttgttgat cacaacacgt ttagcctgac
     4441 ccaggtcttc cagggttgct ttttccagct ccataccgat ctcttcagag attacggtac
     4501 cgccagtcag ggttgcgata tcctgcagca tagctttacg acgatcgccg aagcccggag
     4561 ctttaaccgc agcaactttc acgatgccac gcatggtgtt aacaaccaga gttgccagcg
     4621 cttcgccttc tacatcttca gcgatgatca gcagcggttt gcctgctttg gcaacagctt
     4681 ccagaaccgg cagcatttcg cggatgttgg agattttctt gtcagccagc aggatgaacg
     4741 ggctttccag ttctactgcg ccagtttccg gcttgttgat gaagtaagga gacaggtagc
     4801 cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc ctgcagaccg gtaccgtctt
     4861 caacggtgat aacgccttct ttaccgactt tgtccatcgc ttcagcgatc agtttaccta
     4921 cggtttcgtc ggagttagcg gagatggtac caacctgagc aatcgcttta gagtcagagc
     4981 acggtacgga cagcgctttc agttcttcaa ctgcagcggt aacagctttg tcgataccac
     5041 gtttcaggtc catcgggttc atgcccgcag caacagcttt cagaccttca gtgatgatag
     5101 cctgagccag tacggttgca gtggtggtac cgtcgcctgc agcgtcgttc gctttagagg
     5161 caacttcttt caccatctgc gcacccatgt tttcgaactt atcttccagt tcgatttcac
     5221 gagcaacgga aacaccatct ttggtgatgg tcggtgcacc gaaagattta tccagaacta
     5281 cgttacggcc tttcggaccg agggtaactt tcactgcatc tgccagtacg tttacgccgc
     5341 gcagcatttt cacacgagcg tcgttaccga attttacgtc tttagctgcc attatcttta
     5401 ttccttaaat tcgtatgttc agtgtcgtgc gcggattacg cttcaacaat tgccagaatg
     5461 tcgctttcgg acatgatcaa cacttcttca ttgtcgatct tctcagattt cacaccgtag
     5521 ccatcgttga aaataacgat gtcgccaact ttcacatcca gcggcttcac ttcgccattt
     5581 tcaaggatac ggccattgcc gacagccagc acttcgccgc gggtggattt agccgctgca
     5641 gagccggtca gaacgatgcc gccagcagat ttagtttcaa cttctttacg cttgacgatc
     5701 acgcgatcat gcaatggacg aatattcatt gataactctc ctttgagaaa gtccgtatct
     5761 gttatgggtg acgccggagc ttacgtggtt tcccggctgg tgaccagaga aatggggatg
     5821 aggcttcgcc ccttcaaggg ggaaaaagaa aaaaaattct gatcagcaca aaatcgggtg
     5881 aaaaccctga ttcacctcac atttcatcgc aatttcttca tcaccgtcat aagcgaatct
     5941 gattgtgcta ccatcgaaaa tctacgcagt tgcccataat ttgggcgcaa tcggacatca
     6001 acccatgagt agactcaaac aagaactggg gctggcccag ggcattggcc tgctatcgac
     6061 gtcattatta ggcactggcg tgtttgccgt tcctgcgtta gctgcgctgg tagcgggcaa
     6121 taacagcctg tgggcgtggc ccgttttgat tatcttagtg ttcccgattg cgattgtgtt
     6181 tgcgattctg ggtcgccact atcccagcgc aggcggtgtc gcgcacttcg tcggtatggc
     6241 gtttggttcg cggcttgagc gagtcaccgg ctggttgttt ttatcggtca ttcccgtggg
     6301 tttgcctgcc gcgctacaaa ttgccgccgg gttcggccag gcaatgtttg gctggcatag
     6361 ctggcaactg ttgttggcag aactcggtac gctggcgctg gtgtggtata tcggtactcg
     6421 cggtgctagt tccagtgcta atctacaaac agttattgcc ggacttatcg tcgcgctgat
     6481 tgtcgctatc tggtgggcgg gcgatatcaa acctgcgaat atccccttcc ctgcacctgg
     6541 taatatcgaa cttaccgggt tatttgctgc gttatcagtg atgttctggt gctttgtcgg
     6601 tctggaagca tttgcccatc ttgcctcgga atttaaaaat ccagagcgtg attttcctcg
     6661 tgctttgatg attggtctgc tgctggcagg attagtctac tggggctgta cggtagtcgt
     6721 cttacacttc gacgcctatg gtgaacaaat ggcggcagca gcatcgcttc caaaaattgt
     6781 agtgcagttg ttcggtgtag gagcgttatg gattgcctgc gtgattggct atctggcctg
     6841 ctttgccagc ctcaacattt atatacagag cttcgcccgc ctggtctggt cgcaggcgca
     6901 acataatcct gaccactacc tggcacgcct ctcttctcgt catatcccga ataatgccct
     6961 caatgcggtg ctcggctgct gcgtggtgag cacgttggtg attcatgctt tagagatcaa
     7021 tctggacgca cttatcatct atgccaatgg catctttatt atgatttatc tgttatgcat
     7081 gctggcaggc tgtaaattat tgcaaggacg ttatcgacta ctggcggtgg ttggcgggct
     7141 gttatgcgtt ctgttactgg caatggtcgg ctggaaaagt ctctatgcgc tgatcatgct
     7201 ggcggggtta tggctgtttc tgccaaaacg aaaaacgccg gaaaatggca taaccacata
     7261 atccggcgtt tcgacattaa tcctggcgat cgtctttatg atcaaggcgg tcgcggtcat
     7321 catcctttcg ctggtactca ccatcaaaag tattaccgcc accggtcccg gcgctaaaac
     7381 cgccgcctgg catgcgagaa aagcgcaaat gtggcatcaa cttcactgtc agatgctttt
     7441 gcaccggcgg taataaaagc agaagaccaa ggaagtcggt gaaaaagccc ggtaataaaa
     7501 gcagcaaacc ggcaatgatc agcgaaacac ttttaatcat ctccgccgct gggttttcac
     7561 ccgccgccat tttttgctgc atcagcacga aattcttaaa gccctggtta cgtaccagtg
     7621 acataccgat aactgacgtg aatataacca gcacgagggt cagcaatacc cccaatacat
     7681 gggcaacctg aataaagatt gaaatctcaa tatagacata aaggaaaatg gcaataaaag
     7741 gtaaccagcg caaaggtttc tcctgtaata gcagccggtt aaccccggct acctgaatgg
     7801 gttgcgaatc gcgttcagct tatattgtgg tcattagcaa aatttcaaga tgtttgcgca
     7861 actatttttg gtagtaatcc caaagcggtg atctatttca caaattaata attaaggggt
     7921 aaaaaccgac acttaaagtg atccagatta cggtagaaat cctcaagcag catatgatct
     7981 cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa aacattcgaa accacatata
     8041 ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag aaggttcaca tgtcaaacaa
     8101 cattcgtatc gaagaagatc tgttgggtac cagggaagtt ccagctgatg cctactatgg
     8161 tgttcacact ctgagagcga ttgaaaactt ctatatcagc aacaacaaaa tcagtgatat
     8221 tcctgaattt gttcgcggta tggtaatggt taaaaaagcc gcagctatgg caaacaaaga
     8281 gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt gccgcatgtg atgaagtcct
     8341 gaacaacgga aaatgcatgg atcagttccc ggtagacgtc taccagggcg gcgcaggtac
     8401 ttccgtaaac atgaacacca acgaagtgct ggccaatatc ggtctggaac tgatgggtca
     8461 ccagaaaggt gaatatcagt acctgaaccc gaacgaccat gttaacaaat gtcagtccac
     8521 taacgacgcc tacccgaccg gtttccgtat cgcagtttac tcttccctga ttaagctggt
     8581 agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa gctgtcgaat tccaagacat
     8641 cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg atgaccctcg gtcaggaatt
     8701 ccgcgctttc agcatcctgc tgaaagaaga agtgaaaaat atccaacgta ccgctgaact
     8761 gctgctggaa gttaaccttg gcgcaacagc aatcggtact ggtctgaaca cgccgaaaga
     8821 gtactctccg ctggcagtga aaaaactggc tgaagtcact ggcttcccat gcgtaccggc
     8881 tgaagacctg atcgaagcga cctctgactg cggcgcttat gtaatggttc acggcgcgct
     8941 gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac ctgcgcttgc tctcttctgg
     9001 tccacgtgcc ggcctgaacg agatcaacct gccggaactg caggcgggct cttccatcat
     9061 gccagctaaa gtaaacccgg ttgttccgga agtggttaac caggtatgct tcaaagtcat
     9121 cggtaacgac accactgtta ccatggcagc agaagcaggt cagctgcagt tgaacgttat
     9181 ggagccggtc attggccagg ctatgttcga atccgttcac attctgacca acgcttgcta
     9241 caacctgctg gaaaaatgca ttaacggcat cactgctaac aaagaagtgt gcgaaggtta
     9301 cgtttacaac tctatcggta tcgttactta cctgaacccg ttcatcggtc accacaacgg
     9361 tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt gtacgtgaag tcgttctgga
     9421 acgcggtctg ttgactgaag cggaacttga cgatattttc tccgtacaga atctgatgca
     9481 cccggcttac aaagcaaaac gctatactga tgaaagcgaa cagtaatcgt acaggg
//
LOCUS       DAPOZO010000145         9273 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPOZO010000145 DAPOZO010000000
VERSION     DAPOZO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.145"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(116..192)
                     /locus_tag="ROI21_004336"
     tRNA            complement(116..192)
                     /locus_tag="ROI21_004336"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Met,seq:cat)
     gene            complement(208..282)
                     /locus_tag="ROI21_004337"
     tRNA            complement(208..282)
                     /locus_tag="ROI21_004337"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(248..250),aa:Gln,seq:ttg)
     gene            complement(317..391)
                     /locus_tag="ROI21_004338"
     tRNA            complement(317..391)
                     /locus_tag="ROI21_004338"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(357..359),aa:Gln,seq:ttg)
     gene            complement(415..499)
                     /locus_tag="ROI21_004339"
     tRNA            complement(415..499)
                     /locus_tag="ROI21_004339"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(463..465),aa:Leu,seq:tag)
     gene            complement(508..584)
                     /locus_tag="ROI21_004340"
     tRNA            complement(508..584)
                     /locus_tag="ROI21_004340"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(548..550),aa:Met,seq:cat)
     gene            complement(964..2628)
                     /gene="asnB"
                     /locus_tag="ROI21_004341"
     CDS             complement(964..2628)
                     /gene="asnB"
                     /locus_tag="ROI21_004341"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDX5113181.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(2885..3637)
                     /gene="nagD"
                     /locus_tag="ROI21_004342"
     CDS             complement(2885..3637)
                     /gene="nagD"
                     /locus_tag="ROI21_004342"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDX5113182.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(3685..4905)
                     /gene="nagC"
                     /locus_tag="ROI21_004343"
                     /gene_synonym="nagR"
     CDS             complement(3685..4905)
                     /gene="nagC"
                     /locus_tag="ROI21_004343"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDX5113183.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            complement(4914..6062)
                     /gene="nagA"
                     /locus_tag="ROI21_004344"
     CDS             complement(4914..6062)
                     /gene="nagA"
                     /locus_tag="ROI21_004344"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDX5113184.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANVGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            complement(6122..6922)
                     /gene="nagB"
                     /locus_tag="ROI21_004345"
     CDS             complement(6122..6922)
                     /gene="nagB"
                     /locus_tag="ROI21_004345"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDX5113185.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            7255..9201
                     /gene="nagE"
                     /locus_tag="ROI21_004346"
     CDS             7255..9201
                     /gene="nagE"
                     /locus_tag="ROI21_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDX5113186.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAIPNAVSVAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
BASE COUNT         2189 a         2355 c         2418 g         2311 t
ORIGIN      
        1 gtacgaggat tcgaacctcg gaatgccgga atcagaatcc ggtgccttac cgcttggcga
       61 taccccaaca aattggtttt tgaatttgcc gaacatattc gacacattca gaatttggtg
      121 gctacgacgg gattcgaacc tgtgacccca tcattatgag tgatgtgctc taaccaactg
      181 agctacgtag ccagattgtt tcttcgatgg ctggggtacc tggattcgaa ccagggaatg
      241 ccggtatcaa aaaccggtgc cttaccgctt ggcgataccc caataaccgg gcggtgaacc
      301 gcttactcga agaagatggc tggggtacct ggattcgaac cagggaatgc cggtatcaaa
      361 aaccggtgcc ttaccgcttg gcgatacccc atccgtacaa cgctttctgg tgaatggtgc
      421 gggaggcgag acttgaactc gcacaccttg cggcgccaga acctaaatct ggtgcgtcta
      481 ccaatttcgc cactcccgca aaaaagatgg tggctacgac gggattcgaa cctgtgaccc
      541 catcattatg agtgatgtgc tctaaccaac tgagctacgt agccatcttt ttttcgcgat
      601 accttatcgg cgttgcgggg cgcattatgc gtatagagcc ttgcagcgtc aacctctttt
      661 tcaaggaaaa ttgctcgaaa gtgactgttt ggttaggttg cgaacagcgt ggcgctatat
      721 tcgtcaatta ttgtttactt tgtgtttttt ccccacccta cagccattct tttgtcatac
      781 aggatgaaat tcggaattca acaatagtgg tgggaaatta atcaatgaaa tactggccta
      841 cagtgatgag ttgtcaaaca gtgatgtggc aaacccggaa catttcctta ctgcatatca
      901 gaatcaacaa gcagctcaat aactgaaact gccccggatt tctccggggc agtaccgcat
      961 ttcttactta tacgccgact ggtgaacacc aaccgcacga ccagacggat cgtccatttt
     1021 cttgaacgcc tcatcccact cgatcgcttt agcggaagaa cacgcgacgg aagggccgcc
     1081 cggcacgcac tcagcggcgc tcggaagcgg gaacagttct tcaaagatct cccggtacag
     1141 atacgcttct ttggacgtcg gcgtgttgta cgggaagcgg aagcgggcag tttccagctg
     1201 ttgatcagaa acctgctgcg ccgctacttc tttcagggtg tcgatccaac tgtaaccgac
     1261 gccatcggag aactgctctt tctgccgcca ggcaacactt gcaggcagat acgcttcaaa
     1321 acattcacgc aggatgtgtt tttccatttt gccgttaccg cacattttat cctgtgggtt
     1381 aatacgcatc gccacatcaa ggaatttttt gtcgaggaac ggaacgcgtg cttccacgcc
     1441 ccaggctgac atcgctttgt tggcacgcgc gcagtcatac atatgcaggg ccagcagttt
     1501 acgcaccgtc tcttcatgca gttctttggc attcggtgct ttgtggaagt aaagataacc
     1561 gccaaacact tcatcagaac cttcaccgga cagcaccatt ttaatgccca tcgccttgat
     1621 cttacgcgac attaaataca tcggtgttga agcgcgaata gtggtcacat cataggtttc
     1681 gatgtggtaa atcacgtcgc ggatggcgtc cagaccttcc tgtacagtga agtgaatttc
     1741 gtgatgcaca gtgcccagat ggtttgccac ttcctgggct gccttcagat ccggtgaacc
     1801 cggcagacct acagcaaagg agtgtaactg cggccaccag gcttcagaac gttcctgatc
     1861 ttccacgcga cgggcggcgt atttcttggt gatcgcggaa ataattgagg aatccagacc
     1921 accagaaagc agcacaccgt aaggcacatc agacatcaaa tggcttttaa cggaatcttc
     1981 cagtgcctga cgcagctcgt ttttgtcggt cacgttatct ttcactgcat caaagtcgaa
     2041 ccagtcgcgg tgataataag aacggatttc gccgtcctga ctccacaaat agctccccgc
     2101 cgggaactct ttaatcgtgc ggcataccgg caccagggct ttcatttctg aggccacata
     2161 cagctgacca tgttcgtcat agcccatata cagtgggatg atccccagat ggtcgcgacc
     2221 aatcagatac gcatcttttt cgctgtcgta cagggcaaag gcaaacatgc cctgcaagtc
     2281 gtcgagaaat tccggccctt tttcctgata cagcgcgagg ataacttcac agtcagaccc
     2341 ggtctggaac tggtaacgat cgccatattc agcgcgcaat gcctggtggt tgtagatttc
     2401 accgtttact gccagcacgt gggttttttg ttggttgtag agaggttgcg cccccgcgtt
     2461 aacgtcaaca atggacagac gttcgtgggc gagaatggcg ttatcgctgg cataaatacc
     2521 ggaccagtcc gggccacgat gacgcatcag gcgtgacagc tcgagtgctt tcttgcgcag
     2581 ctcaactgcg tctgttttga tatcgaatac gccaaaaatt gaacacataa ccttctccgt
     2641 taacctggta tttgttgctt gttgtgtttg cttgtttaaa aaaatgccgc aaagcagcac
     2701 tgtgcgcaag cgatttggca gggggtaaat aaaaaacgta atggtgattg ctgattaatg
     2761 aaaaagggta gggtgtggag gtgtatttat tgatgaatcg ataatttttg gcgggtttta
     2821 ttgaatgggc gaattttagt gatgggtaaa aataagaaac cacgccctga ggcgtggttg
     2881 ctattcagat aacgtcgatt tcggcgaccg atgggtaaat ccagctgggg cggaatggca
     2941 tactgtcgat atcgtcgagc gacgaaacac cggagagtac cagaatcgtc tccagaccgg
     3001 cctggaagcc cgccagaata tcggtacgca ggttatcgcc aacaatcacc gtttcttccg
     3061 aatgcgcctg cattttgttt aatgctgcgc ggatgatcca cggactcggc ttaccaacat
     3121 agaacggttt acgtccggag attttttcga tccccgcgca tagcgcgcca caggccggat
     3181 aaaaaccgcg cccgtgggtg tccgggttgg tggcgataaa gcgtgcaccg ttagcgacga
     3241 aataggctgc tttatgcatc atgtcccagt tgtaggaacg cgtttcgcca acaatcacaa
     3301 aatcagggtt cacatcggta atagtgaaac cggctttgta cagttcatga atcagtgcgc
     3361 cttcgcccac cacatatgct ttcttgcctt cctggcgacg caggaaatcg gcggttgcca
     3421 tcgcggaggt ataaaacacg ctgtccggca catcgacacc cgcagtggca aagcgattcg
     3481 ccagatcttg cccggtctgt gaaggatagt tggtcagcaa caccagcggc aggcctttat
     3541 ccataatccc gtgcaaaaat tccgctgcac ccggtacggc gacgttatcg tgcatcagca
     3601 cgccgtcgat atcgcaaatt acatttttaa tggtcatgga ctacccagaa tattgacaac
     3661 aataagcgcc actataaaag cacattaatt ttccagcaaa tgctggagca aaataccgtt
     3721 gagcatggcg cgttttacca gcgcaaaagc gccgattgcc gagcggtgat ccagctcaga
     3781 acgtaccacc ggcagattag tgcgaaacgc cttcagcgcc tgggtattaa tgcagctttc
     3841 aatagcaggg agcagcactt tatcggcttc ggtgatttct ccggcaataa caattttttg
     3901 cgggttaaac aggttgatgg caatggcgat ggttttgccc agatgacgac cgacatactc
     3961 aatcacttcc gacgccaggc tatcgccttt gttcgcagct ttgcagatag tttttatggt
     4021 gcagtcgtcc agcggcacgc ggctctggta gccctgcttt aacagattca acacccgttg
     4081 ttcaatagcg gcgttggcag cgatagtttc caggcagcca aagttgccgc agtggcagcg
     4141 ttcacccagc ggttcgacct gaatatggcc aatttcaccg acgttaccgt tgcggccaat
     4201 aaaaatgcgc ccgttagaga taatcccggc cccggttccg cgatgtacac gcaccagaat
     4261 ggagtcttcg caatcctgac ttgcaccgaa gtagtgctcc gccagcgcca gactgcggat
     4321 atcgtggccg acaaaacagg tcactttaaa acgctcttcc agtgcttcta caagccccca
     4381 gttttccacc tgaatatgcg gcatgtaatg aattttgccg ctgtccgggt caacaagccc
     4441 cggcaggatc acggaaatgg cgatcagctc gcgcagcttg cgctggtagc tatcaataaa
     4501 ctgagcaatg gcattcagca gggcatgttc cagcgtctgc tgggtacgtt ccggcaacgg
     4561 gtaatgttct tctgccagca ctttgctgct gagatcaaac agggtgatgg tggcgtcgtg
     4621 acgaccaagc cgtacgccga ttgcgtggaa attgcgggtt tcggtgacga tagagatagc
     4681 gcggcggccc ccggtggagg cctgctgatc aacttcttta atcagcccgc gttcgataag
     4741 ctgacgcgta attttggtta cgctggcggg ggcaagctgg ctttgctcgg caatctgaat
     4801 ccgcgagatt ggcccatact ggtcaatcag gcgataaacc gccgcgctgt taagctgttt
     4861 tacgagatca acattaccta tctgagcttg tccgcctggt gtcatacttt ctcttattga
     4921 gttacgacct cgttaccgtt aacgatggtc ctggtgattt taaaatcagg tgtgaatgcg
     4981 gtcaggttgg ctactttacc tgcggcgagt gtgccgagac gtttctcaac gccaatcgca
     5041 cgcgccggat agagcgtcgc catacgcagc acttcatcca gtgcgatacc gcaatgttcg
     5101 accagattac gcacgccttc aatcatggtt aaggatgaac cgcttaacgt accgttctca
     5161 tccacacaaa gtccattacg gtagtatatt gttttacccg caaaaatgaa ctgttcaatg
     5221 ttggcacctg ctggcgcggt ggcgtcagta accagacaca gtttgtcgcc tttcagacgt
     5281 ttagcgttgc gaatgttggc gtaatcaaca tgcaggccat cagcgataat accgcaataa
     5341 atgtcagctt cgtcgaggat cgcgcccgcc aggcccggct cacgaccggt aatatacggc
     5401 attgcgttgt acagatgggt ggcaaaggta atccccgcgc ggaaaccggc tttcgcttct
     5461 ttcaacgtcg cgttggagtg accggcagaa accacaatcc cgacatttgc cagtttgctg
     5521 atgacttctg caggaaccat ttccggtgcc agggtcactt tggtaatgac atcggcattt
     5581 tcgcacagga aatcgaccag cgcggcatca ggcttacgca caaaattcgg attatgggtg
     5641 ccttttttta ccagattcag ccacgggcct tccagatgca gacctaacgc ctgattcgga
     5701 tgttttgcca ggtactcgcg cataacgcgc acgccctgtt tcatcagctc gtcgctggtg
     5761 gtgataagcg tcggcaaata gttagtacag cctgatttct cattggcttt ctgcatgatt
     5821 tccagcgttt ctacgctgac tgcttcagca gtatcgttaa actgcacgcc gccgcagccg
     5881 tttaactgca catcgataaa accgggggag agaatggccc cgttcagtga acgttgttcg
     5941 atctctggcg gcagttccgc taccggacag acgcttttaa tcaggccatc agcgataaca
     6001 accgcgtggt catcaagaaa ttcgtggccg gtaaagatcc ggccctgggt taatgcatac
     6061 attctgaccc ccgattttaa aaaatattgc cctgagcaag gtgtcagggc agggataaca
     6121 attacagacc tttgatattt tctgcttcta attcattgaa atatcttaaa gtcttaactt
     6181 tcagctccat ggtggaaggt tcatcgcaca ccatgatcgc ttttggatgc agttgcagac
     6241 agctgatggt ccacatgtgg tttacgcaac cttcaacggc ggcctgcagt gccagtgctt
     6301 tctggctacc cagcaccaga atcatcactt cttcggcatc cagcaatgta ccaacaccga
     6361 cagtcagggc atattttggc acctgattga catcgttatc aaagaaacga gagttcgcga
     6421 cgcgagtgtc atgagtcagg gttttgatac gagtacgaga agccagagaa gacgccggtt
     6481 cgttaaatgc aatatgaccg tcgttaccta caccgcccat aaacagatga atttttccgt
     6541 aagaacggat tttttcttca tactggcggc actcggcgtc gatatccggg gcgttgccgt
     6601 tgagaaggtt gatgttttct gctggaatat caacgtgatc gaagaaatta cggtgcataa
     6661 agctgtagta gctttccgga tgctctttcg gcagaccgac atattcgtcc atgttgaagg
     6721 tgacaacgtg cttaaagctg acctggcctg ctttatgcat ttcgactaac gctttatagg
     6781 tggtcatcgg cgtgccgcca gtcggcaggc ccagaacaaa cggacgatcg gcagtcggtt
     6841 tgaacgcatt gatacgattg acgatatggc gagcagccca tttgccgacc tgttcagcgg
     6901 tagtcagggg gatcagtctc attattcacc tcaataagta aaatgtaagc cgttggcgga
     6961 ttaggcatct ttaagcgtaa cctggatttg cgcagacagg cgtcaatccg acctgatttt
     7021 ttgaatgata aaataagttt tctggtttag ccagtaaaag ggagtgatga taacgatatt
     7081 tggtgacaaa actcacaaaa gacacgcgtt taatttgcga tacgaattaa attttcacac
     7141 actctgtagc agatgatcta acaatctgat tacagaacat cggcagtaca atttgcagca
     7201 aaataaaaat acggctttaa acgagccaaa tagggttctc gtagggggaa taagatgaat
     7261 attttaggtt ttttccagcg actcggtagg gcgttacagc tccctatcgc ggtgctgccg
     7321 gtggcggcgc tgttgctgcg attcggtcag ccagatttac ttaacgttgc gtttattgcc
     7381 caggcgggcg gtgcgatttt tgataacctc gcattaatct tcgccatcgg cgtggcatcc
     7441 agctggtcga aagacagcgc aggtgcggcg gcactggcgg gtgcggtagg ttactttgtg
     7501 ttaaccaaag cgatggtgac catcaacccg gaaattaaca tgggtgtact ggcgggtatc
     7561 attaccggtc tggtcggtgg tgcagcctat aaccgttggt ccgatattaa actgccggac
     7621 ttcctgagct tcttcggcgg caaacgcttt gtgccgatcg ccaccggctt cttctgtctg
     7681 gtgctggcgg ccatttttgg ttacgtctgg ccgccggtac agcacgctat ccatgcaggc
     7741 ggcgagtgga tcgtttctgc gggcgcgctg ggttccggta tctttggttt catcaaccgt
     7801 ctgctgatcc caaccggtct gcatcaggtg ctgaacacca tcgcctggtt ccagattggt
     7861 gaattcacca acgcggcggg aacggttttc cacggtgata tcaaccgctt ctatgccggt
     7921 gacggcaccg cggggatgtt catgtccggc ttcttcccga ttatgatgtt cggtctgcca
     7981 ggtgcggcgc tggcgatgta cttcgcagca ccgaaagagc gtcgtccgat ggttggcggg
     8041 atgctgcttt ctgttgctgt tactgcgttc ctgaccggtg tgactgagcc gctggaattc
     8101 ctgttcatgt tccttgcgcc gctgctgtac ctcctgcacg cactgctgac cggtatcagc
     8161 ctgtttgtgg caacgctgct gggtatccac gcgggcttct ccttctctgc gggggctatc
     8221 gactacgcgt tgatgtataa cctgccggcc gccagccaga acgtctggat gctgctggtg
     8281 atgggtgttg tcttcttcgc tatctacttc gtggtgttca gtttggttat ccgcatgttc
     8341 aacctgaaaa cgccgggtcg tgaagataaa gaagatgaga tcgttactga agaagccaac
     8401 agcaacactg aagaaggcct caatcaactg gcgaccaact atattgctgc ggttggcggc
     8461 actgacaacc tgaaagcaat tgacgcctgt attactcgtc tgcgcctgac cgtggttgac
     8521 tcggctcgcg tcaacgatgc gatgtgtaaa cggctgggtg cttctggggt agtgaaactg
     8581 aacaaacaga ctattcaggt gattgttggc gcgaaagcgg aatccattgg cgatgcaatg
     8641 aagaaagttg ttgcccgtgg tccggtagcc gctgcgtcag ctgaaactgc gcctgcaact
     8701 gccgcgcctg tagcaaaacc gcaggctata ccaaacgcgg tgtctgtcgc ggagctggta
     8761 tcgccgatta ccggtgatgt tgtggcactg gatcaggttc ctgacgaagc attcgccagc
     8821 aaagcggtgg gtgacggtgt ggcggtgaaa ccgacagata aaatcgtcgt atcaccagcc
     8881 gcagggacaa tcgtgaaaat cttcaacacc aaccacgcgt tctgtctgga aaccgaaaaa
     8941 ggcgcggaga tcgtcgtcca tatgggtatc gacaccgtag cgctggaagg taaaggcttt
     9001 aaacgtctgg tggaagaggg cgcgcaagta agcgcagggc aaccgattct ggaaatggat
     9061 ctggattacc tgaacgctaa cgcccgttcg atgattagcc cggtggtttg cagcaatatc
     9121 gacgatttca gcggcttgat cattaaagct cagggccatg ttgtggcggg tcaaacaccg
     9181 ctgtatgaaa tcaaaaagta atctgcttta tacctgatgc gacacggcag cgtcgcatcc
     9241 aacaatgaca agcggtggag atcttctctg ccg
//
LOCUS       DAPOZO010000146         9196 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPOZO010000146 DAPOZO010000000
VERSION     DAPOZO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.146"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            117..1091
                     /gene="cysB"
                     /locus_tag="ROI21_004347"
     CDS             117..1091
                     /gene="cysB"
                     /locus_tag="ROI21_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDX5113187.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1386..1550
                     /locus_tag="ROI21_004348"
     CDS             1386..1550
                     /locus_tag="ROI21_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDX5113188.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1553..1720
                     /locus_tag="ROI21_004349"
     CDS             1553..1720
                     /locus_tag="ROI21_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113189.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1834..1929
                     /locus_tag="ROI21_004350"
     CDS             1834..1929
                     /locus_tag="ROI21_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113190.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2093..4768
                     /gene="acnA"
                     /locus_tag="ROI21_004351"
     CDS             2093..4768
                     /gene="acnA"
                     /locus_tag="ROI21_004351"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDX5113191.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIYALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDIYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLIGEEKIDIVDLQSLQPGATVPVTFTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4832..5422)
                     /gene="ribA"
                     /locus_tag="ROI21_004352"
     CDS             complement(4832..5422)
                     /gene="ribA"
                     /locus_tag="ROI21_004352"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDX5113192.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5592..6356
                     /gene="pgpB"
                     /locus_tag="ROI21_004353"
     CDS             5592..6356
                     /gene="pgpB"
                     /locus_tag="ROI21_004353"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDX5113193.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6505..6813
                     /gene="lapA"
                     /locus_tag="ROI21_004354"
     CDS             6505..6813
                     /gene="lapA"
                     /locus_tag="ROI21_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDX5113194.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            6820..7989
                     /gene="lapB"
                     /locus_tag="ROI21_004355"
     CDS             6820..7989
                     /gene="lapB"
                     /locus_tag="ROI21_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDX5113195.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8180..8917
                     /gene="pyrF"
                     /locus_tag="ROI21_004356"
     CDS             8180..8917
                     /gene="pyrF"
                     /locus_tag="ROI21_004356"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDX5113196.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8917..>9196
                     /gene="yciH"
                     /locus_tag="ROI21_004357"
     CDS             8917..>9196
                     /gene="yciH"
                     /locus_tag="ROI21_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDX5113197.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIHRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLK"
BASE COUNT         2261 a         2144 c         2426 g         2365 t
ORIGIN      
        1 ctataaatga tatagtggtt atagttagca ccttttttat tattaaatcg tattagtcac
       61 ccgccaggtg tgacgaaaaa acgatgttct gatggcgtct aagtggatgg tttaacatga
      121 aattacaaca acttcgctat attgttgagg tggtcaatca taacctgaat gtctcatcaa
      181 cggcggaagg actttacaca tcacaacccg ggatcagtaa acaagtcaga atgctggaag
      241 acgagctagg cattcaaatt ttttcccgaa gcggcaagca cctgacgcag gtaacgccag
      301 caggacaaga aataattcgt attgctcgtg aagtcctgtc gaaagtcgat gccataaaat
      361 cggttgccgg agagcacacc tggccggata aaggctcgct gtatatcgcc accacgcata
      421 cccaggcacg ctatgcgtta ccaaacgtca tcaaaggctt tattgagcgt tatccccgcg
      481 tttctttgca tatgcaccag ggctcgccga cacaaattgc tgatgctgtc tctaaaggca
      541 atgccgattt cgctatcgcc acagaagcgc tgcatctgta tgaagattta gtgatgttac
      601 cgtgctacca ctggaatcgg gctattgtag tcactccgga tcacccgctg gcaggcaaaa
      661 aagccattac cattgaagaa ctggcgcaat atccgttggt gacatatacc ttcggcttta
      721 ccggacgttc agaattggat actgccttta accgcgcagg gttaacgccg cgtatcgtct
      781 tcacggcaac ggatgctgac gtcattaaaa cttacgtccg gttggggctg ggggtagggg
      841 tcattgccag catggcggtg gatccggtcg ccgatcccga ccttgtgcgt gttgatgctc
      901 acgatatctt cagccacagt acaaccaaaa ttggttttcg ccgtagtact tttttgcgca
      961 gttatatgta tgatttcatt cagcgttttg caccgcattt aacgcgtgat gtcgttgatg
     1021 cggctgtcgc attgcgctcc aatgaagaaa ttgaggtcat gtttaaagat ataaaactgc
     1081 cggaaaaata atttcttgcg ttattttcgg caccttttat gtagcgaagg tgccggaata
     1141 tattctcttt tgttacttat ttgtataccg tgcacaatat ttttatccct catatttata
     1201 gggtaaatta cctgtaaagt agtgtctaat tacccgcctc gcaaattcca gttttcaaat
     1261 atctatttca ggtcaaaaga ttgaattatt cactggagac gattcgtaaa ttcgctggat
     1321 ttttgactaa agtttcttta ggatttgtct catcgtatga ttaattacac caatcgttca
     1381 acgtcttgct gataagtgat ggcgattata tgaggttagc aatgccttct ggaaatcagg
     1441 aaccccgccg ggaccctgaa ttaaaacgca aagcctggct ggcggtattt cttggttctg
     1501 ctcttttctg ggtggttgtc gcactgctga tttggaaagt gtggggataa ctatggtcgg
     1561 tcaggagcaa ctggagtcgt caccattatg tcagcatagt gataatgaac cagaaacgaa
     1621 acgggaatgt tccgtcgtta ttccagacga ctggcaacta acatcgcagc agcaagcctt
     1681 tatagaactg tttgctgaag atgatcagcc gaaacaataa ttatcatcat tcttattacc
     1741 catttttaat gaattaaatg gcttttaata caccgcagca ataacaggtt gagttatctc
     1801 aacacaaagt aataaccgtt aagggtgtag cctatgatca acacaaatat gaaatattgg
     1861 tcctggatgg gcgcattttc tctgtcgatg ctcttctggg ccgaactcct ctggatcatt
     1921 actcactgat ccctgacccc gctgcggcgg ggttttcatt tgttttgcca caagctttct
     1981 cctcttttat caatttgggt tgttatcaaa tcgttacgcg atgtttgtgt tatctttaat
     2041 attcaccctg aagagaatca gggcttcgca accctgtcat taaggaggag ctatgtcgtc
     2101 aaccctacga gaagccagta aggacacgtt gcaggccaaa gataaaactt accactacta
     2161 cagcctgccg cttgctgcca aatcactggg cgatatcacc cgtttgccta agtcactcaa
     2221 agttttgctc gaaaacctgc tgcgctggca ggatggtaac tcggttaccg aagaggatat
     2281 ctacgcgctg gcgggatggc tgaaaaatgc ccatgctgac cgtgaaattg cctaccgccc
     2341 ggcaagggtg ctgatgcagg actttaccgg cgtacctgcc gttgttgatc tggcggcaat
     2401 gcgcgaagcg gttaaacgcc tcggcggcga tactgcaaag gttaacccgc tctcaccggt
     2461 cgacctggtt attgaccact cggtgaccgt cgatcgtttt ggtgatgatg aggcatttga
     2521 agaaaacgta cgcctggaaa tggagcgcaa ccacgaacgt tatgtgttcc tgaaatgggg
     2581 aaagcaagcg ttcagtcggt ttagcgtcgt gccgccaggc acaggcattt gccatcaggt
     2641 taacctcgaa tatctcggca aagcagtgtg gagcgaattg caggacggtg aatggattgc
     2701 ttatccggat acactcgttg gtactgactc gcacaccacc atgatcaacg gccttggcgt
     2761 gctggggtgg ggcgttggtg gcatcgaagc cgaagccgca atgttaggcc agccggtttc
     2821 catgcttatc ccggacgtag tgggctttaa actcaccgga aaattacgtg aaggtattac
     2881 cgccacagac ctggttctca ccgttaccca aatgctgcgc aaacatggtg tggtggggaa
     2941 attcgtcgaa ttttatggtg atgggctgga ttcactaccg ctggcggatc gcgccaccat
     3001 tgccaatatg tcgccagaat atggtgccac ctgtggcttc ttcccaatcg atgctgtcac
     3061 cctcgattac atgcgtttaa gcgggcgcag cgaagatcag gtcgagttgg tcgaaaaata
     3121 cgccaaagcg cagggcatgt ggcgtaaccc gggcgatgaa ccgattttta ccagtacgtt
     3181 agaactggat atgaatgacg ttgaagcgag cctggcaggg ccaaaacgcc cacaggatcg
     3241 cgttgcactg cccgatgtac caaaagcatt tgccgccagt aacgaactgg aagtaaatgc
     3301 cacgcataaa gatcgccagc cggtcgatta tgttatgaac ggacatcagt atcagttacc
     3361 tgatggcgcg gtggtcattg ctgcgataac gtcgtgcacc aacacctcta acccaagtgt
     3421 gctgatggcc gcaggtttgc tggcgaaaaa agctgtaaca ctgggcctca agcggcaacc
     3481 gtgggtcaaa gcgtcgctgg caccgggttc gaaagtcgtt tctgattatc tggcaaaagc
     3541 gaaattgaca ccgtatctcg atgaactggg gtttaacctt gtgggatatg gttgtaccac
     3601 ctgtattggt aactctgggc cgctgcccga tcctatcgaa acggcaatca aaaaaggcga
     3661 tttaaccgtc ggtgcagtgc tgtccggcaa ccgtaacttt gaaggccgta tccatccgct
     3721 ggttaaaacc aactggctgg cctcgccgcc gctggtggtt gcctatgcgc tggcgggaaa
     3781 tatgaatatc aacctcgcat ctgagcctat cggccatgat cgcaaaggcg atccggtata
     3841 tctgaaagat atctggccat cggcacaaga aattgcccgt gcggtagaac aagtctccac
     3901 agaaatgttc cgtaaagagt acgcagaagt ttttgaaggc acagcagagt ggaaggaaat
     3961 taacgtcgca cgatccgata tctacggttg gcaggaagat tcaacctata ttcgcttatc
     4021 gcctttcttt gatgaaatgc aggcaacacc agcaccagtg gaagatattc acggtgcgcg
     4081 gatcctcgca atgctggggg attcagtcac cactgaccat atctctccgg cgggcagtat
     4141 taagcccgac agcccggcgg gtcgatatct acaaggtcgg ggtgttgagc gaaaagactt
     4201 taactcctac ggttcgcggc gtggtaacca tgaagtgatg atgcgcggca ccttcgccaa
     4261 tattcgcatc cgtaatgaaa tggtgcctgg cgttgaaggg gggatgacgc ggcatttacc
     4321 tgacagcgac gtagtctcta tttatgatgc tgcgatgcgc tataagcagg agcaaacgcc
     4381 gctggcggtg attgccggga aagagtatgg atcaggctcc agccgtgact gggcggcaaa
     4441 aggtcctcgt ctgcttggca ttcgtgtggt gattgccgaa tcgtttgaac gaattcaccg
     4501 ttcgaattta attggcatgg gcatcctgcc gctggaattt ccgcaaggcg tgacgcgtaa
     4561 aacgttaggg ctaatcgggg aagagaagat tgatattgtc gatctgcaaa gtctacaacc
     4621 cggcgcgacg gttccggtga cgtttacgcg cgcggatggt agccaggaag tcgtaccctg
     4681 ccgttgtcgt atcgacaccg cgacggagtt gacctactac cagaacgacg gcattttgca
     4741 ttatgtcatt cgtaatatgt tgaagtaacg agtatttgct tgccggttat tttgcttccg
     4801 gcaagcaaat gaattacaca atgcaagagg gttatttgtt cagcaaatga cccattttct
     4861 cggctttggt atcgagataa tgttcgttat tggggttacg acctacaatc aacggtacgc
     4921 gttcaacaat attaatccct gcttcggtca gaatttcgac ttttttcggg ttattggtta
     4981 acaagcggac ttcattgacg ccaaggagtt tgaacatatc agcgcaaaga gtgaagtcgc
     5041 gctcatcagc ggcgaagcct aactggtggt tagcctctac ggtatcgtaa ccttgatcct
     5101 gcagtgcata agcgcggatt ttattcagca gaccaatgtt acgaccttcc tgacggtgat
     5161 acagcaaaat accacggcct tcttcggcaa tttgcgtcag tgccgcttcg agctggaagc
     5221 cacaatcgca gcgcaagctg aacagggcgt caccggtcag acattcggaa tggacgcgcg
     5281 caagtaccgg ggtatgcccg gatatatcgc catagactag cgcgacatga tcgtgtccgg
     5341 ttgccagttc ttcaaatccc accatcagga aatcgcccca tggggttggc agtttggctt
     5401 ctgccacacg tttaagctgc atgaatttct ccagataatg ctggttctgt attggcttat
     5461 tttgccataa cgagaagggg ttcacctaat cacgacgcgt cgatcgttca cggaatggca
     5521 caaatctgtc aatttttcct ggaactggca ttttcagtta tgattgtggg acttatcaaa
     5581 aaggagaggc catgcgttcg attgccagac gtaccgcagt gggagctgca ctattgcttg
     5641 ttatgccagt agccgtatgg atttctggct ggcgttggca acctggagaa caaagttggc
     5701 tactaaaagc ggctttttgg gttactgaaa ccgtcaccca gccctggggc gtcattacac
     5761 atttgatttt attcggttgg tttctctggt gtctgcgttt tcgcattaag gctgccatta
     5821 tgttatttgc cattctggcg gcggcaattc ttgtgggaca aggcgttaaa tcctggatca
     5881 aagacaaagt ccaggaaccg cgaccttttg ttatctggct ggaaaaaaca catcatattc
     5941 cggttgatga gttctacact ttaaagcgag cagaacgcgg aaatctagtg aaagaacagt
     6001 tggctgaaga gaaaaacatc ccacaatatt tgcgttcaca ctggcagaaa gagacggggt
     6061 ttgcctttcc ttccggtcac acgatgtttg ctgccagttg ggcactgctg gccgttggtt
     6121 tgctgtggcc gcgtcggcga acgctaacca ttgctatctt gttggtctgg gcaacgggag
     6181 tcatgggaag ccgcctgctg ctcgggatgc attggccacg cgatctggtg gtagctacgt
     6241 tgatttcgtg ggcgctggtg gcggtggcaa cgtggcttgc gcaacgaatt tgtgggccat
     6301 taacaccacc tgcggaagaa aatcgcgaaa ttgcacaacg agaacaagaa agttaacgct
     6361 ggttgatttt ccgaatttag cccttaaatc atcaacaatg cgcgtggatg ccatttcgca
     6421 gacggcgcga aaatggtact ttaaagggct attgcggtaa gttgaccata atttattcgc
     6481 tctaaccaca taacgggaag taatgtgaaa tatttactca ttttcttact ggtgttagcg
     6541 atcttcgtga tttcggtcac gttaggtgcg cagaacgatc aacaggtgac gtttaattat
     6601 ctgttagcgc aaggggagta ccgtatttcc acattgctgg cggtattgtt tgctgcgggg
     6661 tttgctatcg gttggctgat ttgcggtctg ttctggctgc gagttcgtgt ttccctggcg
     6721 cgcgctgaac gtaaaataaa gcgactggaa aaccagcttt cacccgcgac tgacgtggct
     6781 gtagcgccgc attcgtcagc ggcgaaggaa taactttcta tgctggagtt gttgtttctg
     6841 cttttgcctg tagccgctgc ctatggctgg tatatgggcc gcagaagtgc gcaacaaaac
     6901 aagcaagatg aagccaaccg cttgtcgcgt gattacgtag cgggggttaa cttcctgctt
     6961 agtaatcaac aggataaagc ggtagacctg tttctcgata tgcttaaaga ggatacgggt
     7021 accgttgaag cccaccttac gctcggaaac ctgttccgtt cgcgtggcga agttgatcgc
     7081 gctattcgca tccatcagac cctaatggaa agcgcctcgc tgacctatga acagcgtctg
     7141 ttggcgattc aacaattggg gcgtgattac atggccgccg ggttatacga ccgcgcggaa
     7201 gacatgttca atcagctgac cgatgaaact gacttccgca ttggcgcgct gcaacagttg
     7261 ctacaaatct accaggctac cagcgagtgg cagaaagcaa ttgatgttgc cgaacgcctg
     7321 gtgaagctgg gtaaagataa acagcgcgtc gaaattgccc atttctactg tgagttagcc
     7381 ctgcagcata tggccagcga cgatctcgat cgtgccatga ccttgctaaa aaaaggggct
     7441 gcggcagata aaaatagcgc ccgcgtatcc atcatgatgg gacgcgtgtt tatggcgaaa
     7501 ggagaatacg ccaaagccgt cgaaagtttg caacgcgtga tatcccagga cagagaactg
     7561 gtcagcgaaa cgctggaaat gttgcaaacc tgctatcagc agttgggtaa aactgccgaa
     7621 tgggcagaat tcctgcagcg cgcggtggaa gagaacaccg gtgccgatgc tgaattgatg
     7681 cttgcggata ttatcgaagc gcgcgacggt agtgaggccg cacaggtcta tattacgcgt
     7741 cagcttcagc gtcatccgac catgcgtgtg ttccataagc taatggatta ccacttaaat
     7801 gaagcggaag aagggcgtgc caaagagagt ctgatggtgc tgcgtgacat ggttggcgag
     7861 aaggtgcgta gtaagcctcg ttatcgctgc cagaaatgtg gttttaccgc atacaccctc
     7921 tactggcatt gtccgtcttg tcgggcctgg tcaaccatta aaccgattcg cggtcttgat
     7981 ggcctgtaat ttttaaaaaa atccgacttt agttacaaca tactaataat taatgttcat
     8041 tgtgctccgg taactcaggc gcagaaaagc ctgccatggg agaaatcgca actgttaatt
     8101 ttttatttcc acgggtagaa tgctcgccgt ttacctgttt cgcgccactt ccggtgccca
     8161 tcatcaagaa ggtctggtca tgacgttaac tgcttcatct tcttcccgcg ctgttacgaa
     8221 ttctcctgtg gttgttgccc ttgattatca taatcgtgat gacgcgctgg cctttgtcga
     8281 caagatcgac ccacgcgatt gtcgtctgaa ggtcggcaaa gagatgttta cattgtttgg
     8341 gccacagttt gtgcgcgaac ttcaacagcg tggttttgat atctttcttg acctgaaatt
     8401 ccacgatatt cccaacactg cagcgcacgc tgtcgctgct gcagctgact taggcgtgtg
     8461 gatggtgaat gttcacgcct ccggtggggc gcgtatgatg accgcagcgc gtgaggcact
     8521 ggttccgttt ggcaaagatg caccgctttt gattgcggtg acagtattga ccagcatgga
     8581 agccagcgac ctggccgatc ttggcgtgac actgtcacct gcagattatg cagaacgtct
     8641 ggcggcactg acgcaaaaat gtggccttga tggtgtggtg tgttctgctc aggaagctgt
     8701 gcgctttaaa caggtattcg gtcaggagtt caaactggtt acaccgggca ttcgtccgca
     8761 ggggagtgaa gcaggtgacc agcgccgcat tatgacgcca gaacaggcgt tggcggctgg
     8821 tgttgattat atggtgattg gtcgcccggt aacgcaatcg gtagatccag cgcagacgct
     8881 gaaagcgatc aacgcctctt tacagcggag tgcatgatga gtgattccaa cagccgtctg
     8941 gtctactcaa cggaaaccgg acgtattgat gagccaaaag cggcccctgt acgccctaaa
     9001 ggcgacggtg tggtgcgtat tcatcgtcag accagtggac gtaaaggtaa gggcgtttgc
     9061 ctgattaccg gtgtcgatct cgatgatgcc gaactgacga aactggcagc ggaactgaag
     9121 aaaaaatgcg gctgcggcgg agcagttaaa gatggaatta ttgaaatcca gggcgataag
     9181 cgtgatttat taaaat
//
LOCUS       DAPOZO010000147         9182 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPOZO010000147 DAPOZO010000000
VERSION     DAPOZO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.147"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            295..450
                     /locus_tag="ROI21_004358"
     CDS             295..450
                     /locus_tag="ROI21_004358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113198.1"
                     /translation="MAALVVTWFNPVKEAFYMHLPAPGKTKKVALVDCMRKLLTILNA
                     MLRKNEE"
     gene            1261..2244
                     /locus_tag="ROI21_004359"
     CDS             1261..2244
                     /locus_tag="ROI21_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDX5113199.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQSAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKISATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEGSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            2316..3464
                     /locus_tag="ROI21_004360"
     CDS             2316..3464
                     /locus_tag="ROI21_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HDX5113200.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            3488..5164
                     /locus_tag="ROI21_004361"
     CDS             3488..5164
                     /locus_tag="ROI21_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HDX5113201.1"
                     /translation="MKLFKSILLIAACHAAQASAVIDINADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            5174..5914
                     /gene="kdsB"
                     /locus_tag="ROI21_004362"
     CDS             5174..5914
                     /gene="kdsB"
                     /locus_tag="ROI21_004362"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDX5113202.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVVGV
                     AEVWVATDDQRVEKAVQAFGGKSIMTRNDHESGTDRLVEVMHKVEADININLQGDEPM
                     IRPRDVGTLLQGMRDDPALPVATLCHAISAEEATEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMNAGINIRTFEI
                     AATGPGVDTPACLEKVRALRAQELAENA"
     gene            5911..7938
                     /locus_tag="ROI21_004363"
     CDS             5911..7938
                     /locus_tag="ROI21_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDX5113203.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQNVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNAALADQAREAMHTIVTGDLSKYNLAPAFVADESERADIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMEENPQAEIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLQRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLCTATNRLSYSRRCTAATACVVWGVKGEQQWLAEAQQKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKIILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGNIPTELIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAGWGLTVDEHHCPRRERRLTIADLIYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            7973..>9182
                     /locus_tag="ROI21_004364"
     CDS             7973..>9182
                     /locus_tag="ROI21_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDX5113204.1"
                     /translation="MQGNALTILLSGKKYLLLQGPMGPFFNDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRALHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLDERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLVKTQVNGGYYGGV
                     KFENALGMNKD"
BASE COUNT         2438 a         2175 c         2288 g         2281 t
ORIGIN      
        1 aacaaataca aaataatcta tataaaacaa tttgttatac aatcagtttc attccgattc
       61 cgggcaccac taattcttaa gaacccgccc acaaggcggg tttttgcttt tggatcttta
      121 tataaaataa acttgggtgc ataacgccat caggtcttaa acactcccga atcatcaata
      181 aacatccccc gctagcaaaa cacagttcag ttcacataac atccctgtta tcccacacat
      241 atctgctggc cgaacgacgc cagtgcgcgt acggaggcaa gagcagccct gcacatggca
      301 gcgcttgtgg taacctggtt taatccggtg aaagaggcgt tttacatgca tctgccagca
      361 cccggaaaaa ccaaaaaagt ggcgctggtt gactgtatgc gtaaattgct gaccatcctg
      421 aacgcaatgc tcagaaagaa cgaagagtga aatgaatcgt agcatcatgt tgctccataa
      481 tttttacgtt caagacagtt gattatcggc gcatatgact caaaaagagc ctgatgattg
      541 cctcttttcc ataggctctt tctctgaagt gaaggaaggc ccggaagaaa gcactcgctg
      601 gcatacgtga ctccagctca accggagata atcaccattg ttatttttct atttaataat
      661 aatctcctga aaaatagata tagcaaaaat acaatccggc cagatttaat tccgcattca
      721 ttaccagaaa taatcaacac cacaactatc atatcactca atagtcaccg ctaaaatttt
      781 attattttcc tcttctttac atagaaattg tcaacctgtt tattatgaat cacttaaata
      841 attaatcaca aaacaacatc accattattt gcgtgtttta tttattaata acatagtcat
      901 acgttctttt tttacaagga aataatcaat tgataaattg tttttggcat taatagtcaa
      961 gtaggaaaca ttttaataaa tgataaaaat cctaaattcc ttgttcataa tgtaggagtg
     1021 tgttaaatag gtgggcaaaa tttggttccc tttctcgcct gtaatattgc agcgtcttcg
     1081 cacctccatg agacattgcg acttaataag aaggtgataa gtcctgcata taagcatgga
     1141 ctgaccatgg ttttatattc attaaaaatt ttgttacaac ccattgattt agcataaata
     1201 aattatagtg ggttcaggtt tgttgtgact gtggcattat ttccgtgcaa aggagctgat
     1261 atgtctgaaa gacatttacc tgatgaccag agcagtacta tcgatccata tctaattacc
     1321 tctgttcgcc agactctggc agaacaaagc gcagcattac aaaacttgtc taaacaactg
     1381 gattccgggc agtaccagcg tgtccttaat ttgataatga actgtaaagg gcacgttatt
     1441 ctttcgggaa tgggtaaatc agggcatgtc ggtcgtaaaa tatcagcgac gctggcctct
     1501 acgggtacgc ctagtttctt tattcatcct gcagaagctt tccatggcga tctgggcatg
     1561 attacgcctt acgatcttct gatccttatt tctgccagcg gtgaaacgga tgaaatcctc
     1621 aagctagttc cttcactgaa aaatttcggc aatcgaatta tcgccattac caataatgga
     1681 aattccacgc tggcgaaaaa tgctgatgcc gtgttggaac tccacatggc gaatgaaacc
     1741 tgcccgaata atcttgcacc aacaacgtct accacgctga cgatggcgat cggcgatgcg
     1801 ctggcgattg ccatgatcca ccaacgcaaa tttatgccga atgattttgc gcgctatcac
     1861 ccgggcggtt cattaggtcg tcgcctgctg acccgcgttg ctgatgtcat gcagcatgat
     1921 gttcctgcgg tacagctgga tgcgtcattt aaaaccgtga ttcaacgtat caccagcgga
     1981 tgccagggaa tggtgatggt agaagacgca gaaggtgggc tagcgggcat tatcaccgac
     2041 ggtgacctgc gtcgttttat ggaaaaagag ggttctctga catccgctac ggctgcgcag
     2101 atgatgacac gtgaaccgct gacgctaccg gaagacacca tgatcattga agcggaagaa
     2161 aaaatgcaaa agcaccgcgt ctcaacgtta ttggtgacca acaaggcaaa taaagtcact
     2221 ggccttgtgc gcattttcga ctaattaagc aacggggttc gagaggttag tctctcattg
     2281 agtttgcgcc ccactgaaac tttgataatc gttacatgtt gataaaagtg aagtctgccg
     2341 tatcctggat gcgtgctcgt ctgtctgcca tctcactggc agatatccaa aagcacctgg
     2401 caaaaatcat cattctggca ccgatggcgg tgctgctgat ctatctggct atcttcagcc
     2461 agcctcgcta tatgagcgag tcgaaagtcg ccattaaacg ctcggatgat ttaaacagcg
     2521 gcagcctgaa ttttggtctg cttctgggtg cctctaaccc cagttccgca gaagatgcgt
     2581 tgtatctgaa agagtacatc aactcgccgg atatgctggc ggcgctggat aagcaactaa
     2641 attttcgtga agcgtttagc cacagcgggc tcgattttct taatcatctt agcaaggatg
     2701 aaaccgcaga aggcttcctg aagtactaca aggaccgtat caacgtctcg tatgacgata
     2761 aaaccggatt actgaatatt cagacgcagg gctttagccc ggagtttgcg cttaagttta
     2821 accaaaccgt gctgaaagag tcagagcgct ttatcaatga gatgtcacat cgcatcgcgc
     2881 gtgaccagct tgcctttgca gaaacggaga tggaaaaggc acgccagcgt ctggacgcca
     2941 gcaaagcgga attgctctct tatcaggaca acaacaacgt tctggatcca caggcacagg
     3001 cacaggcggc gagcacgtta gtgaatacgc tgatgggcca gaagatccag atggaagcgg
     3061 acctgcggaa cttgctgacg tatctgcgtg aggacgcccc gcaagttgtg agtgcgcgta
     3121 atgcgattca gtcattgcag gcacaaattg acgaagaaaa aagcaaaatc actgcgccac
     3181 agggtgacaa gctaaaccgt atggcagtgg attttgaaga aatcaaatca aaagtagagt
     3241 tcaacaccga gctgtacaaa ctgaccctga cctccattga aaagacccgt gtagaagcgg
     3301 ctcgtaagct caaggtgctg tcagtgatca gttcgccaca gttgccgcag gaatcgtctt
     3361 ttccaaatat cccttatttg atcgcctgct ggttactggt gtgctgctta ctgttcggca
     3421 ccctgaaact gttgctggct gttattgaag atcaccgaga ctaacgctgt cgctgaatga
     3481 gtttgtgatg aaattattta aatcaatttt actgattgcc gcctgtcacg cggcgcaggc
     3541 cagcgcggtc attgatatta acgctgaccc aaacctgaca ggagccgcgc cgcttaccgg
     3601 tattctgaac ggacagaagt cggatacgca aaacatgagc ggcttcgaca ataccccgcc
     3661 gcccgcaccg ccggtggtaa tgagccgtat gtttggtgct caacttttca acggcaccag
     3721 cgcggatagc ggtgcgacgg taggattcaa ccctgactat attctgaatc cgggtgatag
     3781 cattcaggtt cgcttgtggg gtgcgttcac ctttgatggt gcgttacagg ttgatcccaa
     3841 aggtaatatt ttcctgccga acgttggtcc ggtgaaagtt gctggcgtca gtaatagtca
     3901 gctaaatgcc ctggtcacat ccaaagtgaa ggaagtatac cagtccaacg tcaacgtcta
     3961 cgcctcctta ttacaggcgc agccagtaaa agtgtacgtg accggatttg tgcgtaatcc
     4021 tggtctgtat ggcggtgtga cgtctgattc gttactcaat tatctgatca aggctggcgg
     4081 cgttgatcca gagcgcggaa gttacgttga tattgtggtc aagcgcggta accgcgtgcg
     4141 ctccaacgtt aacctgtacg acttcctgct gaacggcaaa ctggggcttt cgcagttcgc
     4201 cgatggtgac accatcatcg tcgggccgcg tcagcatact ttcagcgttc agggcgatgt
     4261 ctttaacagc tacgactttg agttccgcga aagcagcatt cccgtaacgg aagcgttgag
     4321 ctgggcgcgc cctaagcctg gcgcgactca cattacgatt atgcgtaaac aggggctgca
     4381 aaaacgcagc gaatactatc cgatcagttc tgcgccaggc cgtatgttgc agaacggcga
     4441 taccttaatc gtgagcactg accgctatgc tggcaccatt caggtgcggg ttgaaggcgc
     4501 acactccggt gaacatgcca tggtactgcc ttatggttcc actatgcgtg cagttctgga
     4561 aaaagtccgc ccgaacagca tgtcgcagat gaacgcagtt cagctttatc gcccatcagt
     4621 ggctcagcgt cagaaagaga tgctgaatct ctcgctgcaa aagctggagg aagcatcact
     4681 ttctgcccag tcatccacca aagaagaagc cagcctgcga atgcaggaag cacaactgat
     4741 cagccgcttt gtggcgaaag cacgcaccgt ggttccgaaa ggtgaagtga tcctcaacga
     4801 atccaatatt gattctgttc tgcttgaaga tggcgacgtc atcaatattc cggagaaaac
     4861 atcgctggtt atggttcatg gcgaagtgct gttcccgaac gcggtgagct ggcagaaggg
     4921 tatgaccacc gaggattaca tcgagaaatg tggtggcctg acgcaaaaat cgggtaacgc
     4981 cagaattatc gtcattcgtc agaacggtgc agccgtcaac gcagaagatg tggattcact
     5041 caaaccgggc gatgagatta tggttctgcc gaaatatgaa tcgaaaaata ttgaagttac
     5101 ccgtggtatt tccaccatcc tctatcagct ggcggtgggt gcaaaagtga ttctgtcttt
     5161 gtaaggagtt gaaatgagca aagctgttat tgtcatcccg gctcgttatg gctcatcgcg
     5221 cctgccgggt aagccactgc tcgatattgt tggtaaaccg atgatccagc atgtttacga
     5281 gcgtgcgtta caggtggtgg gcgttgcgga agtttgggtg gcaacagacg atcagcgtgt
     5341 tgaaaaggcc gtgcaggcat ttggcgggaa atccatcatg acgcgcaacg atcatgaatc
     5401 cggcaccgat cggctggtcg aggtgatgca taaagtcgag gcagatatca acattaacct
     5461 gcagggcgac gaaccaatga ttcgcccacg ggatgtagga acgctgttac aaggaatgcg
     5521 tgatgacccc gcgttgccag tggcaacgct atgccacgcg atttctgccg aagaagcgac
     5581 cgagccaagc acggtaaaag tggtggtaaa tacccgccag gatgcgcttt attttagccg
     5641 ttcgccgatt ccctatccgc gtaatgctga aaaagcgcgc tacctgaaac acgttggtat
     5701 ttacgcttat cgtcgcgatg tgctgcaaaa ctacagccag ttaccggagt ccatgccaga
     5761 gcaggcagaa tcactggagc agctacggtt aatgaacgcg gggatcaaca ttcgcacgtt
     5821 tgagattgcc gcaaccggtc cgggcgtcga caccccagca tgcctggaaa aagtgcgcgc
     5881 cctgagggca caggaactgg ctgaaaacgc atgattggca tttactcgcc tggcatctgg
     5941 cgtattccgc atctggagaa atttctggcg caaccatgcc agaaactttc tctgctgcgc
     6001 cctgttccgc aaaacgtcga tgctatcgcc gtgtggggac atcgtcccag cgcggcgaaa
     6061 ccagtcgcca tcgccaaagc agcgggaaaa cccgtcattc gtctggaaga tggatttgtg
     6121 cgttcgctgg atcttggcgt caatggcgag ccgccgcttt ctctggtggt ggatgattgt
     6181 ggcatttact acgatgccag caagccttcg gcgctggaga aactggtaca ggataaagcc
     6241 ggaaatgcgg cattggctga tcaggccaga gaagcgatgc acaccatcgt gaccggggat
     6301 ttgtcgaaat ataacctggc gcctgcattt gtggctgatg agtcagaacg tgcagacatc
     6361 gttctggttg tcgatcagac atttaatgac atgtccgtga cgtatggcaa tgctggcccg
     6421 catgagtttg ctgccatgct ggaagctgcg atggaggaaa atcctcaagc cgaaatttgg
     6481 gtgaaggtgc atccggatgt cctggaagga aagaaaacag gttatttcgc tgatctgcgt
     6541 gccacgcaac gagtacgttt gattgccgag aatgtcagcc cgcagtcgct attgcgacac
     6601 gtttcccggg tttacgtcgt gacatcccaa tacggctttg aagccttgct ggcaggaaaa
     6661 ccagtaacat gtttcggcca gccctggtat gcaggctggg gcttaaccga cgatcgccat
     6721 ccgcagtccg ctttgttatc tgcccgacgc ggttctgcca cgctggaaga actttttgcc
     6781 gctgcatacc tgcgttactg tcgctatatc gacccgctca cgggggccgc aagcgatctg
     6841 tttaccgtac tgcaatggct gcaattacag cgcaaccatc agcaacagcg taatggctat
     6901 ttatgggcgc caggcttaac gctgtggaag tcggcaatcc tgaaaccttt cttgtgtacc
     6961 gcaacaaaca gactgagtta ttcacgccgt tgtactgcgg cgacggcttg cgtggtatgg
     7021 ggtgtaaagg gagaacagca atggctagcc gaagcgcaac aaaaatcact gccgttatgg
     7081 cgaatggaag atggttttct gcgttcatcc ggacttggct ctgacctttt gccgccgcta
     7141 tcgttggtac tggataaacg cgggatctac tatgacgcca cgcgccccag cgacctggaa
     7201 gtcctgctta atcacagcca gttaacgctg gcgcagcaga tgcgagctga aaaattacgc
     7261 cagcgactgg ttgaaagcaa actgagcaaa tacaacctgg gggccgattt ctctctgcct
     7321 gccgaggcca aagataaaaa aatcatcctg gtgccgggtc aggtagaaga cgatgcctca
     7381 attaaaacag gcactgtgtc gattaagagc aaccttgagt tattacgcac agtacgcgag
     7441 cgcaatccgc acgcttacat tatttataaa ccgcacccgg atgtactggt ggggaatcgc
     7501 aagggcaata ttccgacaga actaattgct gaactcgctg attatcaggc actggacgca
     7561 gatattattc aatgcatcca gcgcgcagat gaagtgcaca ccatgacatc attgtccggg
     7621 tttgaagcgt tattacatgg caagcaagtt cattgttacg gcctgccctt ctatgccggt
     7681 tggggtttaa ccgttgatga acatcattgc ccgcgccgcg agcgcagatt aacgatagca
     7741 gacttgatct atcaggcgtt gattgtttac ccaacatata ttcacccaac acggctacag
     7801 cctattacgg ttgaggaagc tgcggaatat ttaatccaga cgccgcgtaa gccgatgttt
     7861 attacccgaa aaaaagcggg gcgggtaata cgctattacc gcaaattaat tatgttctgc
     7921 aaggtcagat ttggctaaac aatttcatag ttgactatta cacagattat ttatgcaagg
     7981 caacgcacta accattttat tatcgggtaa aaaatatctg ctattgcagg gaccgatggg
     8041 accttttttc aatgacgtcg ccgaatggtt agagtcatta ggccgtaacg ctgtgaatgt
     8101 tgtcttcaac ggcggggatc gtttttactg ccgccatcga caatacctgg cttactacca
     8161 aacgccgaaa gagtttcccg gatggttacg ggctctccac cggcaatatg actttgatac
     8221 catcctctgc tttggtgact gccgcccatt gcacaaagaa gcaaaacgct gggcaaagtc
     8281 gaaagggatc cgctttctgg catttgagga aggatattta cgcccgcaat ttataaccgt
     8341 tgaagaaggc ggagtgaacg catattcctc gctaccgcgc gatccggatt tttatcgtaa
     8401 gttgccagat atgcctgcgc cgcacgttga gaacttaaaa ccttcaacga tgaaacgcat
     8461 tggccatgcg atgtggtatt acctgatggg ctggcattac cgtcatgagt tccctcgcta
     8521 ccgccaccac aaatcatttt ccccctggta tgaagcacgt tgctgggttc gtgcgtactg
     8581 gcgcaagcaa ctttacaagg taacacagcg caaggtattg ccgaggttaa tgaatgagct
     8641 ggatcagcgt tattatcttg ccgttttgca ggtgtataac gatagccaga ttcgtaacca
     8701 cagtaattat aacgatgtgc gtgactatat taatgaagtc atgtactcat tttcacgtaa
     8761 agcgccgaaa gaaagttatt tggtgatcaa acatcatccg atggatcgtg gtcacagact
     8821 ctatcgacca ttaattaagc gattgagtaa ggaatatggc ttagatgagc gagtcattta
     8881 tgtgcacgat ctcccgatgc cggaattatt acgccatgca aaagcggtag tgacgattaa
     8941 cagtacggcg gggatctctg cgctgattca taacaaacca ctcaaagtga tgggcaatgc
     9001 cctgtacgac atcaaaggct tgacgtatca agggcatttg caccagttct ggcaggccga
     9061 ttttaaaccg gacatgaaac tgtttaagaa gtttcggggg tatttattgg tgaagacgca
     9121 ggttaatggg ggttattatg gaggagtaaa atttgagaat gcattgggta tgaataaaga
     9181 tt
//
LOCUS       DAPOZO010000148         9070 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPOZO010000148 DAPOZO010000000
VERSION     DAPOZO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.148"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..920
                     /locus_tag="ROI21_004365"
     CDS             <1..920
                     /locus_tag="ROI21_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDX5113205.1"
                     /translation="WVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAVMPYGPKC
                     IYVLEQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQLNAALVND
                     ATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSHQCPTETV
                     PYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAAVLNAEVG
                     CSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVIVAINKDK
                     NAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            976..2265
                     /locus_tag="ROI21_004366"
     CDS             976..2265
                     /locus_tag="ROI21_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDX5113206.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNNDSVSLGLVCGLGDIAQAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            2262..2555
                     /locus_tag="ROI21_004367"
     CDS             2262..2555
                     /locus_tag="ROI21_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDX5113207.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2612..4258
                     /gene="fadK"
                     /locus_tag="ROI21_004368"
     CDS             2612..4258
                     /gene="fadK"
                     /locus_tag="ROI21_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDX5113208.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4315..6693)
                     /gene="ppsA"
                     /locus_tag="ROI21_004369"
     CDS             complement(4315..6693)
                     /gene="ppsA"
                     /locus_tag="ROI21_004369"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDX5113209.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            7026..7859
                     /gene="ppsR"
                     /locus_tag="ROI21_004370"
     CDS             7026..7859
                     /gene="ppsR"
                     /locus_tag="ROI21_004370"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDX5113210.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            8016..9062
                     /gene="aroH"
                     /locus_tag="ROI21_004371"
     CDS             8016..9062
                     /gene="aroH"
                     /locus_tag="ROI21_004371"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDX5113211.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIAGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
BASE COUNT         2178 a         2351 c         2353 g         2188 t
ORIGIN      
        1 tctgggtctt tagcgataat cctgaacgtt atgctgaact gtttggcggc gcgcagcaat
       61 ggggccaaca ggtgtatgcc attgtacaaa ataccgacca ggcgcaggca gttatgcctt
      121 atggtccaaa atgtatttat gttcttgagc aaaatgatgc gctgcaacgt actgaaaatt
      181 acgccgaaag cattgctgcc ctgctgaaag ataaacaccc ttctatgttg ctgctggccg
      241 cgacgaaacg cggtaaagct ctggcagcac ggttaagtgt gcaactgaat gcggcgctgg
      301 tgaacgatgc cactgcggtg gatattgtcg atggtcacat ttgcgccgaa caccggatgt
      361 atggcgggtt agcgttcgca caggaaaaga tcaatagccc gctggcgatc attacccttg
      421 cgcccggtgt tcaggaaccg tgcaccagtg atacctctca tcagtgtccg acagaaacgg
      481 taccttatgt tgccccgcgt catgaaattc tctgtcgcga acgccgtgcg aaagcggcaa
      541 gcagcgtgga cctgagcaaa gcaaaacgtg tggttggcgt cggtcgtggt ctggcagcgc
      601 aggatgacct gaaaatggtc cacgaactgg cggcggtgct gaatgctgaa gtcggctgtt
      661 cacgtccaat tgccgaaggc gagaactgga tggagcgtga acgttatatc ggtgtctccg
      721 gcgtgttgct gaaatccgat ctctacctga cgctggggat ctccgggcag atccaacata
      781 tggttggcgg caacggcgca aaagtgattg tcgccatcaa taaagataaa aatgcgccaa
      841 tcttcaacta tgccgactac ggtctggtgg gcgatatcta caaagtcgtc cctgccctga
      901 ttagccagtt gagccgctaa ttcccctttc aaccacatct cccgctgtaa acgcagcggg
      961 aagggagcat aacgcatgtc ggatgacaaa tttgatgcca ttgtggtcgg tgcgggcgtt
     1021 gctggtagcg ttgccgcact ggtcatggcg cgagccggac tggatgtcct ggtgatagaa
     1081 cgcggcgaca gtgccggatg taaaaacatg accggcgggc gtctttatgc ccacacactt
     1141 gaagcaatca ttccaggctt tgcagcatca gcgccggtag aacgcaaggt cacacgcgag
     1201 aaaatctcct tcttaaccga agaaagcgcc gttaccctcg attttcaccg cgagcagcca
     1261 gatgttccgc aacacgcatc ttataccgta ttgcgtaatc gtctggaccc gtggttgatg
     1321 gaacaagccg agcaggcggg cgcgcagttt atcccaggcg ttcgcgtcga tgcgctggtt
     1381 cgtgaaggaa acaaggtaac tggcgtgcag gctggggatg atattctcga agccaatgtg
     1441 gtgattctgg cagatggcgt taattcaatg cttggccgtt cgctgggaat ggttcccgct
     1501 tccgatccgc atcattacgc tgttggtgtt aaagaggtta ttggcctgac accagaacag
     1561 atcaacgatc gctttaatat tacgggcgag gaaggtgccg cctggctgtt tgccggttcc
     1621 ccttctgacg gcctgatggg tggcggattc ctctatacca acaacgattc cgtatcgttg
     1681 gggctggttt gtggattggg tgatatcgcc caagcgcaaa aaagcgtgcc gcaaatgctg
     1741 gaagatttta aacaacaccc cgccattcgc ccgctgatta gcggcggcaa gttgcttgaa
     1801 tattccgcgc atatggtgcc agaaggcggt ctggcaatgg tgcctcagat ggttaacgat
     1861 ggcgtgatga tcgttggtga cgccgcaggc ttctgcctga atttgggttt tacagttcgt
     1921 ggcatggatt tagccattgc atcggctcag gctgccgcca caacggtgat cgccgccaaa
     1981 gaacgcgagg atttctccgc cagcagtctt gcgcaataca aacgtgagct ggaacaaagc
     2041 tgcgtcatgc gtgatatgca gcattttcgc aagatcccgg cgctgatgga aaacccgcgc
     2101 ctgtttagcc aatacccacg aatggtagcc gacatcatga acgagatgtt caccattgac
     2161 ggcaaaccaa accagccggt acgcaaaatg atcatgggac acgcgaagaa aattgggctg
     2221 atcaacttgc tgaaagatgg cattaaggga gcaaccgcgc tatgagccag aacgctacgg
     2281 ttaacgtcga catcaaatta ggcgtcaata aattccatgt tgatgagggc cacccgcata
     2341 tcattttggc ggcaaatccc gatatcaatg aattccgtaa attaatgaaa gcctgccctg
     2401 ccggacttta taagcaggat gatgccggca acattcattt tgattctgcc ggttgtctgg
     2461 agtgcggcac ctgtcgggtg ctgtgcggta acactattct cgaacagtgg caatatcccg
     2521 caggcacctt cggtattgat tttcgctacg gctaatcatg catcccacag gcccgcatct
     2581 cgggcctgat gctctgtttc gagagtccaa aatgaaagtg acattaacgt ttaacgaaca
     2641 acgtcgtgcg gcgtatcgtc agcaagggct atggggcgat gcttcgctgg ccgattactg
     2701 gcagcagacc gctcgtgcga tgccagacaa aattgccgtg gtcgataatc atggtgcatc
     2761 gtacacctat agcgcgctcg atcacgccgc gagctgtctg gcaaactgga tgttagcgaa
     2821 gggtattgaa tcaggcgatc gcatcgcatt tcaactgcct ggctggtgtg aatttaccgt
     2881 tatctatctt gcctgcctga aaatcggtgc ggtttccgtg ccgctgttgc cttcctggcg
     2941 ggaagcagaa ctggtatggg tgctcaataa gtgtcaggca aaaatgttct ttgctccgac
     3001 gttgtttaaa caaacgcgtc cggtagattt aatcctgccg ctgcaaaatc agcttccaca
     3061 actacaacaa attgtcggcg tggacaaact ggctcccgcc acctctgctc tctcattaag
     3121 tcagattatc gccgacaata ctcctctgac cacggcgata acggtccacg gcgatgaatt
     3181 agccgcggtg ctgtttacct ccggaaccga gggtctgcca aagggcgtga tgctaacgca
     3241 taacaatatt ctcgccagtg agcgggctta ttgcgcgcgg ctgaatctga cctggcagga
     3301 tgtctttatg atgcctgcgc cacttggtca cgcaacgggc tttctgcatg gcgtaacagc
     3361 accattttta attggtgctc gcagcgtgtt gttagatatt ttcactcctg atgcgtgtct
     3421 cgcgctgctt gagcagcagc gttgcacctg tatgctaggc gcaacgccgt ttgtctatga
     3481 tcttttgaat gtactagaga aacaacccgc ggacctgtca gcgctgcgtt tctttctttg
     3541 cggcggaacc acaatcccca aaaaagtggc gcgtgaatgc cagcagctcg gcattaaatt
     3601 attaagtgtt tatggttcca cagaaagttc gccgcatgcg gtggtgaatc tcgatgatcc
     3661 tttgtcgcgc tttatgcaca ccgatggtta cgctgccgca ggtgtagaga ttaaagtggt
     3721 cgatgacgca cgcaagacct taccgccagg ttgcgaaggt gaagaagcct cgcgtggccc
     3781 caatgtgttt atgggatatt ttgatgaacc tgaattaacc gcccgtgcgc tggatgaaga
     3841 aggctggtat tacagcggcg atctctgccg tatggatgag gctggctata taaaaattac
     3901 cggacgcaaa aaagatatta ttgtccgcgg cggcgaaaat attagcagcc gtgaagtgga
     3961 agatatttta ttgcagcatc ctaaaattca cgatgcctgt gtggttgcaa tgcccgatga
     4021 acgtttaggt gaacgatcat gcgcttatgt cgtgctgaaa gcgccgcatc attcattatc
     4081 gctggaagag gtggtggctt tttttagccg taaacgggtc gcaaaatata aatatcctga
     4141 acatatcgtg gtaatcgaaa aactaccgcg cactgcctca ggtaaaatac aaaagttttt
     4201 gttaagaaaa gatattatgc ggcgattaac gcaggatgtc tgtgaagaga ttgaataagt
     4261 ttcatcttcg gggatcacat aaacccggcg actgaacgcc gccggggatt tattttattt
     4321 cttcagttca gccaggctta accaggtttg caccacggtg tccgggttca gagacaggct
     4381 atcgatcccc tcttccatca accatgcggc aaagtcttca tggtcggacg gaccctgacc
     4441 gcaaatcccg acatatttgc cctgtttctt cgcggcacgg atagccatcg acagcaatgc
     4501 tttcaccgca tcgttgcgct catcgaacaa ttcagacacc acgccggagt cacgatccag
     4561 accgagcgcc agctgtgtca tatcgtttga gccaattgag aagccgtcga aatattcgag
     4621 gaactgctct gccagcaagg cgttggacgg aatttcacac atcatgatga ttttcagccc
     4681 gttctcgcca cgtttcagcc cctgacgcgc cagttcttca accaccgctt tcgcctgatc
     4741 tacggtacgc acgaacggga tcatgatctc aacgttggtc agccccatgt cgttgcgcac
     4801 tcgtttcact gcttcacact ccagcgcgaa gcagtcgcgg aagctgtcgg aaacatagcg
     4861 gcccgcgcca cggaagccga gcatcgggtt ctcttcatct ggctcgtaac gctcaccacc
     4921 gaccaggttc gcatattcgt tcgatttaaa atcagagaga cggacaatga cgcgcttcgg
     4981 ataaaacgcg gcacccagcg tcgcgatccc ttcagtcaga cgaccaacgt aaaattcacg
     5041 cggagaatca aaacctttca tcatctcgcg gatttcgttt tgcaactgcg gttcctgatc
     5101 gtcaaactca agcagtgcgc gtgggtggac gccaatcata cggttgatga taaattccag
     5161 acgcgcaagg cccacgcctt cgttcggcag gcaggcgaag tcgaaagcac ggtccgggtt
     5221 accgacgttc atcatcactt tcaacggcag atccggcatc gtttctacgc tggagctttt
     5281 cacgctaaat tccagcaact ccgcatagac gtaaccggta tcaccttcgg cacaagaaac
     5341 agtgacgttc tcaccgtctt tcatccgttc tgttgcatct ccacagccca ctaccgccgg
     5401 aatgcccagt tcacgagcga tgatcgccgc gtgacaggta cgaccgccac ggttggtgac
     5461 gatggcagat gctttcttca tgatcggttc ccagtccggg tcggtcatgt cagtaaccag
     5521 cacgtcgcca ggttcgatgc ggttcatttc gctgatgtca tggatgactt tcaccggacc
     5581 cgcgccgatg cgatgaccga tagcacggcc ttcggcgata atcttaccct gtgaatgcag
     5641 cgtataacgc tccatgacct gaccgcgtga gcgcacggtt tccggacgcg cctgcacaat
     5701 gaacagtttg ccggtgtggc catctttcgc ccactcaata tccattgggc gaccgtagtg
     5761 tttctcaatt tgtacggcct gttttgccag ttcctgcact tcttcgttgg tcagcgagaa
     5821 gatgtcacgc tgttcctgcg gtacgtcttc gattttaacc tgcttgccgt gctcctgggt
     5881 cggcgcgtaa accatgcgga tttttttcga ccccatggtg cggcgcacga tagccgggcg
     5941 attcgccgcc agtgtcggtt tatggacgta aaactcatcc gggttaaccg caccctgcac
     6001 gaccatttca ccaaggcccc atgcggaagt aataaacacc acctggtcaa agccggattc
     6061 ggtatcaatg gagaacatca cgccagatga tgcgaggtca gagcgcacca tccgctgaac
     6121 accggcggag agcgccacac cacggtgatc gtaaccctgg tgcacacgat aagagatggc
     6181 gcgatcgtta aacagagaag caaatacatg cttcactgcc acgagaacgg cgtcaaaacc
     6241 ctgaacgttg aggaaggttt cctgctgacc ggcaaaagaa gcgtccggca tatcttctgc
     6301 ggtggcggag gagcgcaccg caaaagaggc gttttcgtca tcggcggaaa gctgtgcata
     6361 ggcttcgcgg atggcgtttt ccagctcagg ctggaaggga gtgtcgataa tccactggcg
     6421 gatttgcgcg cccgctttcg caagctgggt aacatcgtca atatccgttt tatccagcag
     6481 ttcataaatg cgctggttta cgccgctttg gtccagaaac tggttaaacg cgtcggcggt
     6541 tgtggcgaaa ccattcggaa cggaaacacc cattccggaa aggttagtaa tcatttcacc
     6601 cagggaggca tttttgcccc caaccctgtc tacatcattc atgccgagtt ggttatacca
     6661 aagcaccagc ggtgacgagc cattgttgga catcgaacaa tccttttgtg ataaatgaac
     6721 ggtttgagaa acacatttct gcgcatttat ctttgcatat ttaaccggat gaaaaaaacg
     6781 gtgaatcgtt caagcaaata tattttttta ctttttaaga ctgatcccag cgttgcgcaa
     6841 atctgctctc ttcgacaatt tccacataaa ccatcggtat aaacgaaacc ataaaattga
     6901 aatgctgttt tcataaaaaa taaaattgaa ggttcatttt ataaaccaga cataacgttc
     6961 tacgcttctg tgcgttttta atttatgctt tcatagaatt atgtctgcat cacgggaaga
     7021 acaaaatgga taatgctgtt gatcgccacg ttttttatat ttctgatggt acggcaataa
     7081 ctgcggaggt attaggacac gcagtaatgt cacaatttcc cgtcactatc agcagcatta
     7141 cgctgccgtt tgtcgaaaat gagagccgtg cacgggcagt gaaggatcag attgacgcaa
     7201 tttatcacca gacaggtgtg cgcccgctgg tcttctactc catcgtgttg ccggagattc
     7261 gcgccatcat cttgcaaagt gaaggctttt gccaggatat cgttcaggcg ctggttgccc
     7321 cgctacaaca agagatgaaa ctggatccca cgccgattgc tcatcgtacc catggcctga
     7381 accctaataa cctcaataaa tatgacgcgc gtattgcggc gattgattac accctcgccc
     7441 acgatgatgg catttcgttg cgcaatctgg accaggctca ggtgatcctg ctcggtgttt
     7501 ctcgctgtgg taaaaccccc accagtctgt atctggcaat gcaatttggt atccgcgcgg
     7561 caaactaccc ctttattgcc gacgatatgg ataatcttgt gctacccgcg tcgctcaaac
     7621 cgcttcagca taaattgttc ggcctgacta tcgacccgga acgtctggcg gcgattcgcg
     7681 aggaacgtcg ggagaacagt cgctatgcct cgcttcgtca gtgcaggatg gaagtcgcgg
     7741 aagtggaagc gttgtaccgt aaaaatcaga tcccgtggat taacagtacc aattattcgg
     7801 tagaagagat tgccaccaag atcctcgata tcatgggcct tagccgccga atgtactaga
     7861 gaactagtgc attagcttat ttttttgtta tcatgctaac tacccggcga ggtgtgacac
     7921 acctcgcact tgaaatcagc tgcgattggt ttatcgtgat gcgcatcact tcccggtagt
     7981 cctgccgtag aagcaacaaa tttctgagac ttgtaatgaa cagaactgac gaactccgta
     8041 ctgcgcgtat tgagagcctg gtaacgcccg ccgaactcgc gctacggtat cccgtaacgc
     8101 ctggcgtcgc cacccatgtc accgactccc gccgcagaat tgaaaaaata ctgaatggtg
     8161 aagataagcg actgttggtc attattggcc cctgctcgat ccacgatctc accgctgcaa
     8221 tggagtacgc cacccgtctg cagtcgctgc gcaaccagta ccagtcacgg ctggaaatcg
     8281 taatgcgcac ctattttgaa aaaccacgaa ctgttgtcgg ctggaaaggg ctaatctccg
     8341 atccggattt aaacggcagc tatcgggtaa atcacggtct ggagctggcg cgcaaattac
     8401 ttttacaggt aaatgagctg ggtgttccta ccgcgactga gttcctcgat atggtgaccg
     8461 gtcagtttat tgctgattta atcagttggg gcgcgattgg cgcacgtact accgaaagtc
     8521 agatccaccg tgaaatggct tcggcactct cctgtccggt aggttttaaa aatggtaccg
     8581 atggcaacac gcggattgcc gtggatgcta tccgcgcagc ccgcgccagc catatgttcc
     8641 tctcgccaga caaaaatggt cagatgacca tctatcagac cagcggcaac ccgtatggcc
     8701 acattattat gcgtggcggc aaaaaaccga attatcatgc cgatgatatc gccgcagcct
     8761 gcgatacgct gcacgagttt gatttacctg aacatctggt ggtggatttc agccacggta
     8821 actgccagaa gcagcaccgt cgccagttag aagtttgtga ggatatttgt cagcaaatcc
     8881 gcaatggctc tacggcgatt gctggaatta tggcggaaag tttcctgcgc gaaggaacgc
     8941 aaaaaatcgc cggcggtcag ccgctcactt acggtcaatc gattaccgac ccgtgtctgg
     9001 gctgggaaga taccgaacgc ctggtcgaaa aactcgcctc tgcagttgat acccgtttct
     9061 gaatgcgtgc
//
LOCUS       DAPOZO010000149         8979 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPOZO010000149 DAPOZO010000000
VERSION     DAPOZO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.149"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(7..1383)
                     /gene="trkA"
                     /locus_tag="ROI21_004372"
     CDS             complement(7..1383)
                     /gene="trkA"
                     /locus_tag="ROI21_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDX5113212.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1405..2694)
                     /gene="rsmB"
                     /locus_tag="ROI21_004373"
     CDS             complement(1405..2694)
                     /gene="rsmB"
                     /locus_tag="ROI21_004373"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDX5113213.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMSWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(2740..3687)
                     /gene="fmt"
                     /locus_tag="ROI21_004374"
     CDS             complement(2740..3687)
                     /gene="fmt"
                     /locus_tag="ROI21_004374"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDX5113214.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3702..4211)
                     /gene="def"
                     /locus_tag="ROI21_004375"
     CDS             complement(3702..4211)
                     /gene="def"
                     /locus_tag="ROI21_004375"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDX5113215.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4341..5465
                     /gene="dprA"
                     /locus_tag="ROI21_004376"
     CDS             4341..5465
                     /gene="dprA"
                     /locus_tag="ROI21_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDX5113216.1"
                     /translation="MVDTEIWLRLMSVSSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5437..5910
                     /locus_tag="ROI21_004377"
     CDS             5437..5910
                     /locus_tag="ROI21_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDX5113217.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5939..6481
                     /locus_tag="ROI21_004378"
     CDS             5939..6481
                     /locus_tag="ROI21_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDX5113218.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6486..7058
                     /gene="tsaC"
                     /locus_tag="ROI21_004379"
     CDS             6486..7058
                     /gene="tsaC"
                     /locus_tag="ROI21_004379"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDX5113219.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7063..7881
                     /gene="aroE"
                     /locus_tag="ROI21_004380"
     CDS             7063..7881
                     /gene="aroE"
                     /locus_tag="ROI21_004380"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDX5113220.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALVGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCQQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            7878..8135
                     /locus_tag="ROI21_004381"
     CDS             7878..8135
                     /locus_tag="ROI21_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDX5113221.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLL"
     gene            complement(8111..8665)
                     /locus_tag="ROI21_004382"
     CDS             complement(8111..8665)
                     /locus_tag="ROI21_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDX5113222.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQSNQTQP"
BASE COUNT         2244 a         2187 c         2239 g         2309 t
ORIGIN      
        1 tattaattac aagaagaaag gacttggctg gaagagtctt tcgacgtcgg taataaactt
       61 tttatctgtg aggaacataa ttacgtgatc gccttgctca atgcgcagat tgtcattggc
      121 aatcatcacg tcgtttccac gtaccaccgc tccaataatc gttcctggcg gtagcttgat
      181 ttcatcaatg actctgccga caacgcgtga ggtactttca tcaccgtgag caacggcttc
      241 aatagcttct gctacgccgc ggcgcaatga ggaaacacca acaatatctg cttttcgcac
      301 atggctaagc aacgcggaaa tcgttgcttg ttgcggtgaa atcgcaatat cgatcacgct
      361 cccctgaacc agatccacat aagcgcgacg ctggatcaat accatcacct ttttcgcacc
      421 catacgtttg gcgagcatgg ctgacatgat attggcctcg tcatcgttgg tgacggcaat
      481 aaacagatca acttgatcga tatgttcttc ggccagcagt tcttgatccg atgcatcacc
      541 aaaaaagacg atcgtattct gcaacttttc cgccagttcg gcagcgcgtt gctgattacg
      601 ttcgatgagt ttaacgctgt aatctttttc cagacgacgc gccagccctg caccgatatt
      661 accgccacca accagcatga tccgcttata cggtttttcc agtcgctgta attcactcat
      721 caccgcgcgg atatgctgtg aagcggcaat aaagaacact tcatcaccag cttcaacaat
      781 ggtcgaacct tgcggacgaa tggggcgatc gtggcggaaa attgctgcca cacgagtatc
      841 gatatgtggc atatgttcgc gcatggtcga aagtgcatta ccaatcagcg ggccgccata
      901 ataggcttta accacagcga ggctgacttt accctcagcg aagttcacca cctgcaatgc
      961 gccgggatac tcaatcagtc ggtaaatatt atcgataacc aactgctctg gtgcgatcag
     1021 atgatcaatc ggtacagcat ctgaatgaaa tagtttatcg gcatcgcgca cgtagtctgg
     1081 tgagcggata cgagcgatgc gattaggggt gttgaaaagt gagtaggcta cctggcaggc
     1141 aaccatattg gtttcatctg aactagttac agcaaccagc atatcggcgt cgtcggcacc
     1201 tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc acgactcgca ggtcaaattt
     1261 atcctgcaag gtccgcagac gctcaccgtt ggtatcgaca acagtaatat cgttgttctc
     1321 gccaaccagg ttttccgcca gtgtgccgcc aacctggccg gcacccagaa tgataatttt
     1381 catcagtcgc gacccgttat ctcatcactt tttgattagc ttagcgtaaa agaagccgtc
     1441 gccctcttcg gcaccaggca gattttgctt acccggttgt tctggtgttc ctgtttcgca
     1501 aagttcggca tcagcggtac gttgcaaaaa ggctttaatc tgcaggctat tctcttccgg
     1561 taacaccgaa caggtggcat agaccagggt tccaccggat tttaaatgcg gccaaatggc
     1621 gtcgagaatt tcagactgca attgcgcgag ttccgggata tcgcgatcgc gacgtaacca
     1681 tttaatatcc ggatggcgac gaatcacacc ggttgctgaa caaggcgcat ctaataaaat
     1741 gcgatcaaac tgttgctcgc cacaccattg ggaagggtag cggccatcac cttgtttcac
     1801 ggtcgccttc ataccaaggc gttttaaatt gtcgtaaacg cgagagaggc gctgttcgtc
     1861 aatatcaacc gcaacaacct gcgcttctgg tgccacctca aggatatgcg ttgttttacc
     1921 gccgggggcg gcacaaagat ccaaaatgtg ttcaccgttt tgtggcgcaa gccagctcat
     1981 gcaaccttgt gctgatgcat cctgaacggt aacccatccg tcttcaaaac caggtagcgc
     2041 atgaacaggt gcaggtgttt ccagccgtac agcatcaggg taatccgcat gcgggaaacc
     2101 tttcattcct gcttcatcca gcaatgcaag ccagctgtcg cgggaatgat gcgtacgatt
     2161 aacgcgcagc cacattggcg gacgctggtt attggcttcg acgatggatt gccactgctc
     2221 tggatacgct ttttgcagac gcttcagcaa ccaggaagga tgcagataac gtgcatcact
     2281 ggcattaaac tcggctaata actcttcttg ctgacgctgg aactggcgta atacaccgtt
     2341 tatcaaccct ttaagttgcg ggcgcttaat tgcgatagcg ccttcaaccg tttcagccag
     2401 cgcagcatga ggtggtatgc gggtataaag cagttgatac aaaccaacca taatcaggta
     2461 atgcacagtg cgctgtttgc cggtcatcgg acgggccatt aacttattaa tcagccagtc
     2521 taactgtgaa agcgtacgca gtacgccaaa gcacaactct tgaagaagtg ctttgtcttt
     2581 atcggaaact ttttgctgga gcggtggcag aatgttgctt aatgattgcc cttgctcgac
     2641 gacttgttca acggcctggg ccgccatgct acgtaaatta cgttgttttt tcataagtat
     2701 aaaaatgccc ggcaagaccg ggcttagaag agtggactat cagaccagac ggttgcccgg
     2761 aacaaaccat tcccgacgag agttcaggag gtcttgcgcg ctcatcgctt tcttacccgc
     2821 aggttgtaac gagagcaggt tcaggatgcc atcaccagtc gcaacctgaa tgccttgttt
     2881 gttggcttca aggatcgttc ctggtgcagc gttggttgtc gtatcaatga ccgatgcttt
     2941 ccagacttta accggctgtc cttcaatttc cagccagctc attggccatg gattgaaagc
     3001 gcgaatgcag cgttcaagct gtgctgccga aagtgaccag tcaatacgcg cttcttcttt
     3061 actcaacttt tcggcgtaag tgacaagagt ttcgtcctga acttctggtt tcgccgtgcc
     3121 gtctgccaat tgtttcaacg tggtgataag cccttgtggg ccaagctctg ccagcttgtc
     3181 gtacagcgta ccactggtat cttctgcagt aatcgggcag gagagcttat agagcatgtc
     3241 accggtgtct aaaccaacat ccatttgcat aatggtcaca ccagtttctg catcacccgc
     3301 ccatagtgag cgttggattg gtgcagcacc gcgccagcgt ggcagcagtg aaccatgaac
     3361 gttgatacag ccaagacgcg gcatctccag cactgcttta ggcagaatta agccataggc
     3421 gacgacaacc ataacatcag cctgtagatc ggcgaccagt tgctggtttt cttgtggacg
     3481 cagggaaacc ggttgaaaaa cgggcagacc tttttcctca gccagaactt taaccgggct
     3541 gggcatcagt tttttaccgc gtcctgctgg tcggtctggc tgggtgaaca cgccaacgac
     3601 gttatgacca gaagacaaca gcgcgtcgag atgacgcgct gcaaagtcag gtgtacccgc
     3661 aaaaataata cgtagtgatt ctgacacgtt agttcttatc cttaagcccg ggctttcaga
     3721 cgatccagtt tttcaacttt ctgacgaata cgttgttgtt tcagcggtga cagataatcc
     3781 ataaacagtt tgccgaccag gtgatccatc tcatgctgaa tacagatggc taacagaccg
     3841 tctgcttcca gttcaaatgg tttaccgtcg cggtccaggg cgcgaatttt aactttctct
     3901 gcgcgtggca ctaaagcacg ttgttcaggg atcgacaggc aaccttcttc aatgcctgtt
     3961 tcgccgcttt tttctaaaag ctccggattg attaacacca gccgttcgtc acggttttcc
     4021 gaaacatcaa taacaatgat acgttgatgg atatcaacct gggttgccgc caggccaata
     4081 ccttcttctg cgtacatcgt ctcgaacata tcatcgacga tacgctgaat ttctgcattc
     4141 acttcttcta ccggtttagc aactttgcga agccgctcgt ccggaatatg taacacttgc
     4201 aaaactgaca taaatctcca gagatgtgtt caggagttag aaagattatt tcttctattc
     4261 tagacaaatc cccctctgat tgacagcatc actgaccaat cgcaaagatt gctaaggctg
     4321 cttatggcag ggagataagg atggtcgata cagaaatttg gctgcgttta atgagcgtca
     4381 gcagcttgta cggcgatgat atggtccgta tagctcactg gctggcaaaa cagtcgcata
     4441 ttgatgcggt tgtattgcag caaacagggc ttacattgcg gcaggcacaa cgctttcttt
     4501 catttccgcg aaagagtatc gaaagctcac tttgttggtt ggagcaaccc aaccatcatt
     4561 taatccctgc ggacagcgaa ttttatcctc ctcaacttct tgcgacgaca gattaccccg
     4621 gcgcactgtt tgttgaagga gaactgcacg cgctgcattc atttcagctt gccgtagtgg
     4681 ggagtcgggc gcattcatgg tatggcgagc gatggggacg attattttgc gaaactctgg
     4741 cgacgcgtgg agtgacaatt acgagtggac tggcgcgtgg aatcgatggt gtggcgcata
     4801 aagcggcctt acaggtaaat ggcgtcagca ttgctgtatt agggaatgga cttaatacca
     4861 ttcatccgcg tcgccatgcc cgactggcta ccagtttgct tgaacatggt ggggctcttg
     4921 tctcggaatt tcccctcgat gttcctcccc ttgcttacaa tttcccacga agaaatcgca
     4981 ttatcagtgg tctaagtaaa ggtgtactgg tggtggaagc ggctttgcgc agtggttcgc
     5041 tggtgacagc acgttgtgcg cttgagcagg ggcgtgaagt ttttgccttg ccgggtccat
     5101 tagggaatcc gggaagcgaa gggcctcact ggttaataaa acaaggtgcg attcttgtga
     5161 cggaaccgga agaaattctg gaaaacttgc aatttggatt gcactggttg ccagacgccc
     5221 ctgaaaattc attttattca ccagatcagg aagacgtggc attgccattt cctgagctcc
     5281 tggctaacgt aggagatgag gtaacacctg ttgacgtcgt cgctgaacgt gccggccaac
     5341 ctgtgccaga ggtagtaact caactactcg aactggagtt agcaggatgg atcgcagctg
     5401 tacccggcgg ctatgtccga ttgaggaggg catgccatgt tcgacgtact aatgtatttg
     5461 tttgaaacct atattcacac agaagctgag ttgcgtgtgg atcaagacaa acttgaacag
     5521 gatcttaccg acgcaggatt tgatcgagaa gatatctaca atgccctgct ttggctggaa
     5581 aagcttgcgg attatcagga aggactggca gaaccgatgc aactggcctc tgatccactc
     5641 tccatgcgta tttatacccc tgaagagtgt gaaagactgg atgccagctg tcgtggtttt
     5701 ttgcttttcc ttgagcaaat tcaggtgctt aaccttgaaa cccgtgaaat ggtgatagag
     5761 cgagtgctcg cgctggatac cgcagagttc gacctggaag acctgaaatg ggtaatcctg
     5821 atggtgttgt tcaatattcc gggctgcgaa aatgcgtacc agcaaatgga agaattactc
     5881 tttgaagtga atgaaggtat gctgcattaa tcatttcttt aattcagcat aagttgttat
     5941 ggcgaaatca gcactgttca cggtgcgtaa taatgagtcc tgcccaaagt gcggggctga
     6001 actggtaatt cgatccggga aacacggtcc gtttcttgga tgctcacagt atccggcgtg
     6061 tgactacgtc cgtcctctga aatcttcagc ggatggacat atcgtcaaag ttctggaggg
     6121 gcaggtttgc cctgcatgtg gcgcaaatct ggtattacgc cagggacgct ttggtatgtt
     6181 tattggttgc agtaactacc ctgaatgcga acataccgaa cttatcgata aaccggacga
     6241 aacagcaatt acatgccccc aatgccggac gggccatctg gtccagcgcc gctcccgtta
     6301 tggcaaaacg tttcactcct gtgatcgcta cccggagtgt caatttgcca ttaacttcaa
     6361 acccatagct ggagaatgcc ctgagtgtca ttatccgcta ctcatcgaaa agaaaaccgc
     6421 gcagggtgta aaacactttt gtgccagtaa acaatgtgga aagccggttt cggcggaata
     6481 ataacgtgaa taataacctg caaggagacg ctatcgcagc tgcgatagat gtcctcaatg
     6541 aagaacgtgt catcgcctat ccaacggaag ccgttttcgg tgtcgggtgc gatcctgata
     6601 gcgaaacagc agtgatgcga ctgttggagt taaaacagcg tccggttgat aaagggctga
     6661 ttctaatcgc cgcaaattac gagcagctta aaccctatat tgatgacacc atgctgactg
     6721 acgcgcagcg tgaaactata ttttctcgct ggccaggtcc tgtcaccttt gtttttcccg
     6781 cgcctgcgac aacaccgcgc tggttgacgg gccgctttga ttcgcttgct gtacgagtca
     6841 ccgaccatcc gttggtggtt gctttgtgcc aggcttatgg taaaccgctg gtttctacca
     6901 gtgccaactt gagtggattg ccaccttgtc gaacagtaga cgaagttcgc gcacaatttg
     6961 gcgcggcgtt cccggttgtg cctggtgaaa cgggggggcg tttaaatcct tcagaaatcc
     7021 gcgatgccct gacgggtgaa ctgtttcgac aggggtaaca taatggaaac ctatgctgtt
     7081 tttggtaatc cgatagccca cagcaaatcg ccattcattc atcagcaatt tgctcagcaa
     7141 ctgaatattg aacatcccta tgggcgcgtg ttggcaccca tcaatgattt catcaacaca
     7201 ctgaacgctt tctttagtgc tggtggtaaa ggtgcgaatg tgacggtacc ttttaaagaa
     7261 gaggcttttg ccagagcgga tgaacttact gaacgggcag cgttggttgg tgctgttaat
     7321 accctcaagc ggttagaaga tggacgcctg ctgggtgaca ataccgatgg tataggtttg
     7381 ttaagcgatc tggaacgtct gtcttttatc cgtcctggtt tacgtattct gctcattggc
     7441 gctggtggag catctcgcgg cgtactactg ccactccttt ccctggactg tgcggtgaca
     7501 atcactaatc ggacggtatc ccgcgcggaa gagttggcta aattgtttgc gcacactggc
     7561 agtattcagg cgttgggtat ggacgaactg gaaggccatg agtttgatct cattattaat
     7621 gcaacatcca gtggcatcag tggtgatatt ccggcgatcc cgtcatcgct cattcatcca
     7681 ggcatttatt gctatgacat gttctatcag aaaggaaaaa ctccttttct ggcatggtgt
     7741 cagcagcgag gctcaaagcg taatgctgat ggtttaggaa tgctggtggc acaggcggct
     7801 catgccttcc ttctttggca tggcgttctg cctgatgtag aaccggtcat caaactattg
     7861 caacaggaat tgtcagcgtg aatcaggcca tccagtttcc ggatagggaa gagtgggacg
     7921 agaataaaag atgtgtatgt tttcccgctc tcgtgaatgg tatgcaactg acatgtgcga
     7981 tctctggcga gagtctggcg tatcgcttta ctggagatac gccagaacag tggttagcga
     8041 gttttcgtca gcatcgctgg gacctggaag aagaagcgga aaacttaatt caggaacaaa
     8101 gtgaagatga tcaaggttgg gtctggttac tttgatccag atattcgtcc ttccatttca
     8161 cgtagttatt tgcggaatag cgtaacccag ccttctcttc atcacttaac gggcggatct
     8221 gtttgacggg gctaccgaga tacagatatc cgctctccag ccgtttattt tgtgggacca
     8281 ggctacccgc accaatcatc acatcatctt ctactattgc gccatcaagt aaaattgagc
     8341 ccatcccaac caaaactcga ttgccaatgg tgcagccgtg gagcatcacc ttgtgaccaa
     8401 cagtgacatc ttcgccaatg gttaatgggt tgccatctgg gttgtacgag gatttatgag
     8461 tgacatgcaa catactgcca tcctggatat tggtgcgtgc tccgatctgt acataatgta
     8521 catctccacg aatcacaacg agcggccaga tccccacatc atcagccaga cgaacgtcac
     8581 caatcacgac gctgctatcg tcgatcatta cgcgctgacc gatttgtgga aaaagatcgc
     8641 ggtatgggcg taaaacatca gacataatta cctcaacaat aaatgatttc ctaatgactt
     8701 tgggtgcatt attggccttg tgcaagtctt ttagcatgca aaaaagcacc gttttgtgtg
     8761 cgaatgcagc aaaaaggatg aaaaagcaac aaacagaaaa aaagatcaaa aaagtacttg
     8821 tgcaaaaaat tgggatccct ataatgcgcc tccgttgaga cgacaacgtg aaacacttca
     8881 caagatggtc gtaacaacaa agagaaaaaa tcctgaaatt cagggttgac tctgaaagag
     8941 gaaagcgtaa tatacgccac ctcgcgacag tgagctgaa
//
LOCUS       DAPOZO010000150         8815 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPOZO010000150 DAPOZO010000000
VERSION     DAPOZO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.150"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            7..288
                     /locus_tag="ROI21_004383"
     CDS             7..288
                     /locus_tag="ROI21_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113223.1"
                     /translation="MSVELTDKGGRCAALGMSNGTWFTLLDIPGVETLFNTRKTNDPI
                     DCTRSKARKLADLIEAWEPPDHWFSGIGKSEGKTLLIAFLRNCKGFRTC"
     gene            386..721
                     /locus_tag="ROI21_004384"
     CDS             386..721
                     /locus_tag="ROI21_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDX5113224.1"
                     /translation="MSQTPSDTFKLSYPFTTAAGTRIELVELKRLTVKDLKQVRKISK
                     NPADWDEPLIARSTGLLPEDLDNMDLADYLQLQKRFQLITGMGESNQGADAGAGAAGE
                     MVPVSAGGD"
     gene            645..803
                     /locus_tag="ROI21_004385"
     CDS             645..803
                     /locus_tag="ROI21_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDX5113225.1"
                     /translation="MRATRALTQAQGLLARWFRFQPGEIDALDTDDLEMWLEQAEEQI
                     KSEYGDKS"
     gene            879..3830
                     /locus_tag="ROI21_004386"
     CDS             879..3830
                     /locus_tag="ROI21_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDX5113226.1"
                     /translation="MSGQFSVGVVIGGMIGSTFRSAMSGTRRALDSLSDTSRRLQERQ
                     NALTRATERYGQLGSSRMQHLNSELLRVSRTMEQIERQQRRLSAASATSDALKANRMA
                     LYGQGIEAYGMAQTVYHTVSPAVQQSMSFQDKMIDMSITAKYDNKTRDALAGQIKGWA
                     LKYNQYQDELQEAVGSLISDNIDNVSDIGFLMPDIARAATATRTSAQDWAKVAAVWQN
                     SLKGAARDFGAVQNIMAYAGDQGSFEIPDQVKWMQSLAPMMAGIASGKEAVAEIGASL
                     QIAKIGAGSTDEAANNFKNFLTKIFARDTQKQFADLGIDLQGSIASYKAAGISPIEGM
                     LSVIERYLNAKSPEALAGFKSAMKIKNDTARDEALQALAKNFGLGDMFADMQVMAFIR
                     PMLANMDRYREIRAGALRAADNDLLASAYDQRLKSPLEATKTLMVSSRDLAITLGDQL
                     APSFISLTQELLPLIQGAKHWVATHPQFVSGAFKLISALLAIKIATVGLKLGLNLLIS
                     PFVSVWKNAVLLRGNWLRLSLALGQGGKLRWLVTGFSAVARGARTLSGVLSGGLVRGI
                     MLAGRAVLWIGRALMMNPIGLVITAVAAAAYLIYRNWGAVSGWFKQRWADIQEAFNGG
                     IVGIGKLLINWSPAGLLYKAFAAALKYFGVALPAKFTDFGGHLIDGLINGIKNKWGSL
                     KSSVTGMGDSISSWFKQCWADIQEAFNGGIAGTGKLLINWSPAGLLYKAFAAALKYLG
                     VDLPAKFTDFGGHLVDGLINGIKNKWESLKSSVTGMGDSISGWFSEKLGIHSPSRVFM
                     GFGDNIAQGAAIGLQRTTPLAALAGQRMASELLPKMPVSIQGPEIRDNTSGVRFSMPL
                     PDINGFMSSAKNAIGAVINSLSSMPAIPLSTRASSLPGQRLANEMTPDVPRIPSPEIL
                     AAGYSGRGATATGGGTSGGIQVSFNPQFFLNGRETTAPAGLTGALNMSLHELEKMLER
                     LLAQKQRKEYR"
     gene            3830..4714
                     /locus_tag="ROI21_004387"
     CDS             3830..4714
                     /locus_tag="ROI21_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5113227.1"
                     /translation="MFAVLGDIEFELITYWDGFEATFGVDYAEHARIGGKPGLQFVGD
                     RLDEIQISLVFHQHYCVPDVELARLRTAMKAHQALALVFGNGDYRGWFVITDVTATSE
                     QTDSTGNVLAVSATVSLREYTGDPKNPLQPPAIRTKLPGVGAVSGAIPSPSGVAQFIR
                     NGVNYAKQAQSVLQTTISAVRVTQKMKDNPVVALTRVPGLMSGLGNISGALGKSVPAF
                     NALSESMPDAISLARTASEAATYVQQAQSALSGVDKRNIAGALDTVSGQLNAAGTAFN
                     RMSPGLSAMAARILTRSV"
     gene            4714..4926
                     /locus_tag="ROI21_004388"
     CDS             4714..4926
                     /locus_tag="ROI21_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDX5113228.1"
                     /translation="MFLEHVTRDGERWDSLAWQYYGDPLGYPRIIAANPHVAITPVLP
                     SGLLLLIPVIEAEEARTEEDIAPWLR"
     gene            4914..6068
                     /locus_tag="ROI21_004389"
     CDS             4914..6068
                     /locus_tag="ROI21_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDX5113229.1"
                     /translation="MAEINSTAQVTSALTGVSDVLTPVFTLWYLQKNITSDIAPYVTR
                     VTWSDNIKNESDTIEVELDDTDGRWLDKWYPGKGDTLTLKMGYQGEKLLSCGTFSIDE
                     IEVSSPASVVSIRGVATSVNSALRTKTSRGFENTTLAAVAGRIARKHRLKLVGSIESI
                     RIDRVTQYAETDVGFLRRLASEYGYAVKVVSDQLIFSHLATLRSQEPVRQLKPQDVAR
                     FSLRDTINRVYKSAKVKHQKSSSKKLIVYEADGGTRESDKKLKGGKVTSADSLKVNSR
                     VSDPDSARIKADSALARHNEYQQNGSLTLTGTPQLTAGNKIELVGFGQLSGPWLITTA
                     RHAFDRNSGYTTELEVARGPVTRGKKQKTQKLTVYHPDGSTSTVIKEKKK"
     gene            6065..6661
                     /locus_tag="ROI21_004390"
     CDS             6065..6661
                     /locus_tag="ROI21_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDX5113230.1"
                     /translation="MTGVTRQVGTVSAVDADRVQARVRLPECDNLRTNWLNVLQRNTQ
                     DNKDYWLPDVGEQVEVLLDANGEDGVILGAVYSDVDKPPFSDKNIRGTRFADGAEYSY
                     NRKTHTLIIRGGIEHIVIECGADVVLKTQKATIDAPETELTGDLRVRGKLIYEGGMAG
                     SGGKGVTATIHGNIEIKGNAHATGSMLSDGENSSHHSH"
     gene            6716..7063
                     /locus_tag="ROI21_004391"
     CDS             6716..7063
                     /locus_tag="ROI21_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDX5113231.1"
                     /translation="MKTTSVFWQPALQAPGEIVRGLDDIRQAIQIILRTPRGSDPHRP
                     EFGSNLHLYIDWPVDRAIPHVVRESVDAIRRWEPRCQLMSVKPAVDGEHLTLRVSWKG
                     SDGQTRTQELLWR"
     gene            7054..8157
                     /locus_tag="ROI21_004392"
     CDS             7054..8157
                     /locus_tag="ROI21_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDX5113232.1"
                     /translation="MALTEPDFIERDADKITAEMIAKYEADTGKTLYPAQAERLLIDL
                     WAYREMLVRVAVQEAAKQNLVAFAREPMIDYLGELVGVYRLAAQPATTTLQFSVDEAL
                     AIDVLIPAGTRVSASDSVIFATDTDVVLKAGLLLVNVTSTCTEPGTAGNGWQPAQVSQ
                     LLDEIDNVDLLVSNLTASSGGSEQEDDDRLRERIRLAPESFTNAGSRGAYRFHAMQAH
                     PNIVDVAVLSPVPGTVDLYPLLSTGLPDGGVLTLVESFCSDEKVRPLTDTVRAKTPVK
                     VDYTIEARITIYRDQDARSVKDAANSAIQNWVASRAATLGRDIVPSQIISALSVSGVY
                     QVELVTPALRVVAENEWANCTAITLNMTGVSDD"
     gene            8150..8728
                     /locus_tag="ROI21_004393"
     CDS             8150..8728
                     /locus_tag="ROI21_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDX5113233.1"
                     /translation="MTEPLQLPPPLEGDISLRTLGRLAGRLDNIDLSVLMVFLVDIVD
                     SSALPWLGEQFSLSGDGWELAESDDVRRMLIKAAIELHRYKGTPWSIREVIRRFGFGE
                     VDLIEGTGRLSYDGNRSYNGLFVHGDAAAWAVYRVILKQPITNDQAAMLRQTLAAFAP
                     ARCHLASLEYQSVAIRYNNTAIHDGSYNHGSS"
BASE COUNT         2060 a         2274 c         2512 g         1969 t
ORIGIN      
        1 ctgacggtga gtgttgaact gacggataaa ggaggacgat gtgcggcact gggcatgtca
       61 aatggtacgt ggtttaccct ccttgatatt ccgggggtgg aaaccctttt taatacccgt
      121 aaaaccaatg acccgattga ctgcacacgt tcaaaggccc gcaaactggc ggatttgatt
      181 gaagcatggg agcctcccga ccactggttc tccggcatcg gcaaatctga gggaaagacg
      241 cttctcatcg ctttcctgcg taactgcaag gggtttcgca cttgctgaca tcacaggggc
      301 tccggcccct tcttcttaat ctcctttaat atccgtcacg cgcttctccc gacatactgc
      361 cctgaactta cacaggagca caatcatgtc acagacccca tccgatactt ttaaattgtc
      421 ttatcccttc accactgctg caggcaccag aattgagctg gttgaactga aacgcctgac
      481 ggtaaaagac ctgaagcagg tgcgcaaaat cagcaaaaac ccggcagact gggacgaacc
      541 gctgattgcc cgcagtactg gtcttctccc ggaagatctc gacaatatgg atctggctga
      601 ttacctgcag ttacagaaac gatttcagct catcacgggg atgggtgaga gcaaccaggg
      661 cgctgacgca ggcgcagggg ctgctggcga gatggttccg gtttcagccg ggggagattg
      721 atgccctcga tactgacgat ctggagatgt ggctggagca ggctgaagag caaataaaaa
      781 gcgagtacgg cgacaaatca tagtacagac agccgccagt agcggctgtt ctgcgttatc
      841 ccctcacgtc ttttcacctt ccccggaggt taaccactat gtcgggacag ttttcagtcg
      901 gcgttgttat cggcgggatg attggtagca cattccgttc tgcaatgagc ggtacccgcc
      961 gtgcgcttga ttccctgagc gatacctcac gccgcctgca ggaacgtcag aacgctttaa
     1021 cccgtgcaac agaacgttat ggtcaactgg gttcttcccg gatgcagcat ctcaacagcg
     1081 agctgctgcg ggtaagccgc accatggagc aaattgagcg ccagcagcgc cgtctgtcag
     1141 cggcatccgc taccagtgat gcgctgaaag ctaaccgcat ggcgctgtat ggtcagggga
     1201 ttgaagcgta tggcatggca cagactgttt atcatacggt ttcccctgcc gttcagcagt
     1261 ccatgtcttt tcaggacaaa atgattgata tgtcgatcac cgcaaaatat gacaataaaa
     1321 cgcgggatgc acttgccgga cagataaaag gctgggcgct taaatacaat cagtatcagg
     1381 atgagctgca ggaggcggtg ggttcactca tcagcgacaa tattgataat gtgtcagata
     1441 tcggttttct gatgccggat attgcccgcg cggcaacggc aacacgcacg tctgctcagg
     1501 actgggcaaa agtggccgca gtctggcaaa actccctgaa aggtgcggcc agagattttg
     1561 gtgccgttca gaatattatg gcttatgccg gtgaccaggg gtcatttgaa atcccggatc
     1621 aggtcaagtg gatgcagtcc ctggccccaa tgatggcggg tattgccagt ggaaaagagg
     1681 ctgttgctga aatcggggcc agtctccaga tagcaaaaat cggtgcaggt tccaccgacg
     1741 aagcagccaa taattttaaa aactttctta ccaaaatttt tgcccgcgat actcagaaac
     1801 agtttgctga tctgggtatt gatttgcagg gatctattgc gagttataaa gctgcgggga
     1861 tctctccgat tgaagggatg ttgagtgtta tagaacgtta cctcaatgcc aaaagccccg
     1921 aagcgctggc cggcttcaaa tcagccatga aaataaagaa tgatacggca agagatgagg
     1981 cacttcaggc tctggcgaaa aactttggtc tgggcgatat gttcgcggat atgcaggtca
     2041 tggcatttat ccgcccgatg ctggccaaca tggacagata tcgagagatc cgtgccggtg
     2101 ctctcagggc tgcggataac gatttgcttg ccagtgctta tgatcagcgg ctgaaatctc
     2161 cccttgaagc cactaaaaca cttatggtca gcagtcgcga tctggcaatt acgctgggcg
     2221 atcaattagc tccatctttt atttctttga ctcaggaact gcttccactc attcaggggg
     2281 caaaacactg ggtagcgact cacccgcaat ttgtcagtgg ggcttttaag ctcatcagtg
     2341 cgctccttgc gattaagata gcgactgttg gtctcaaact ggggctgaat ctccttattt
     2401 ccccctttgt aagtgtctgg aaaaatgctg ttttacttcg gggcaactgg cttcgtctgt
     2461 cgctcgcact ggggcaaggc ggtaagctcc gctggctggt gaccggattt agcgccgtcg
     2521 ccagaggagc cagaacactg agtggcgtgc tctccggggg gctggttcgc ggaattatgc
     2581 tcgccggacg tgctgttctc tggattggac gtgcgctgat gatgaatccc atcggtctcg
     2641 ttatcaccgc tgtcgcggca gcagcttacc ttatctaccg caactggggg gcagtcagtg
     2701 gctggtttaa acagcgctgg gcagacattc aggaagcctt taacggcggc attgtgggaa
     2761 ttggtaagtt gctgattaac tggtcgccgg caggcctgct ctataaagcc tttgcggctg
     2821 cgctgaaata tttcggcgtt gctctgccgg caaagttcac cgacttcggt ggccatctta
     2881 tcgacggtct gattaacggt atcaaaaaca aatgggggtc gctcaaatcc agtgtaaccg
     2941 gaatgggtga cagcatcagt agctggttta aacagtgctg ggctgacatt caggaagcct
     3001 ttaacggcgg tatcgcggga actggtaagc tgctgattaa ctggtcgccg gcaggtctgc
     3061 tctataaagc ctttgcagct gcgctgaaat atcttggtgt tgatctgccg gcaaagttta
     3121 ccgacttcgg tggccatctt gtcgatggtc tgattaacgg tatcaaaaac aaatgggagt
     3181 cgctcaaatc cagtgtaacc ggaatgggtg acagcatcag tggctggttc agcgaaaagc
     3241 tgggcattca ttcgccgagc cgcgtgttta tgggctttgg tgacaatatc gcgcaggggg
     3301 ccgccattgg cctgcagcgg accactccgc ttgcagctct ggccgggcag cgaatggcca
     3361 gtgaactgct ccccaaaatg cccgtgagca ttcaggggcc agaaatacgg gataacactt
     3421 caggtgttcg cttcagtatg ccgttgcccg atatcaatgg gtttatgtcg tctgctaaaa
     3481 atgcgatcgg agccgtaatc aatagtttgt cttccatgcc cgctattccg ctctccaccc
     3541 gggcatcgag tttaccaggg caacgtctgg caaatgagat gacaccggat gttccccgta
     3601 tcccctcgcc tgaaatcctg gctgccggat attcaggccg tggtgcaact gcaaccggcg
     3661 gtggaacgtc tggtggtatc caggtcagct ttaatcctca gtttttcctc aatggcaggg
     3721 aaaccacagc gcctgcagga ctgactggtg ccctgaatat gagtctgcat gagctggaaa
     3781 aaatgctgga gcgtctgctg gctcagaaac aacgtaagga gtaccgctga tgtttgccgt
     3841 actgggtgat attgagtttg agctgattac ctactgggac ggcttcgagg ccacgttcgg
     3901 cgtcgattat gcggagcatg cccgcatcgg gggtaagcct ggcctgcagt tcgtcggcga
     3961 caggctggac gaaatccaga taagtctggt cttccatcag cattattgtg tacccgatgt
     4021 ggagctggcg agactgcgaa cagccatgaa agcccatcag gcactggcgc tggtcttcgg
     4081 caacggtgac tatcgtggct ggttcgtgat taccgatgtg accgcaacca gcgagcagac
     4141 agacagcacc ggtaacgtgc tggctgtcag tgccaccgtg tctctccggg aatacaccgg
     4201 tgatccgaaa aatcctctgc aaccaccggc aatacgcacg aagctcccgg gtgtcggggc
     4261 ggtctccggt gccattcctt caccttcagg ggtggcgcag ttcatccgca acggcgtcaa
     4321 ctatgcgaaa caggcgcagt ctgtactcca gaccactatc agcgccgttc gggtgacaca
     4381 gaaaatgaag gataaccccg ttgtcgcact gacccgtgtg ccggggctga tgagcggact
     4441 gggtaatatc tccggggctc tggggaaaag tgttccggcg tttaacgcac tctctgaatc
     4501 catgcccgat gccatcagtc tggccagaac agccagcgaa gcagccacgt atgtacagca
     4561 ggcacagtct gcgctgagtg gtgtggacaa aagaaatatt gcaggtgctc tggataccgt
     4621 ttccgggcag cttaacgccg ccggcacagc attcaaccgc atgtctccgg gattaagtgc
     4681 aatggccgcc agaatactga cgaggagtgt gtgatgtttc ttgaacatgt tacccgtgac
     4741 ggagagcgct gggattcgct ggcatggcag tactacggtg acccgctggg ctatccccgg
     4801 attattgccg ccaatccgca cgtggccatc acgccggtgc tgccctccgg gctgttgtta
     4861 ctgatcccgg tgattgaggc tgaagaagcc cgtacagaag aggatattgc cccatggctg
     4921 agataaacag cactgcgcaa gtcacatcag cgttaaccgg cgtcagcgat gtgctgacac
     4981 cggtgttcac tctgtggtat ctgcagaaaa acatcacctc tgatatcgcg ccttatgtca
     5041 cccgtgtgac ctggagcgat aacatcaaaa atgagtccga taccattgag gtggagctgg
     5101 acgacaccga tggccgctgg ctggataagt ggtatccggg caagggtgac acgctgacgc
     5161 tgaaaatggg ctatcagggc gagaagctgc tgtcctgcgg tacgttctct atagacgaga
     5221 tcgaagtgag ttcgcccgct tccgttgttt ctatccgtgg ggtggccacc tcggttaaca
     5281 gtgccctgcg gactaaaacc agtcgtggtt ttgagaacac cacgctggca gctgttgcgg
     5341 ggcggattgc cagaaagcac cgactgaaac tggtgggcag cattgagtcc atcagaatcg
     5401 accgggtgac ccagtatgct gaaaccgacg tgggttttct gcgccggctg gccagcgagt
     5461 atggttatgc agtgaaagtg gtcagtgacc agctgatttt ttctcatctg gccacactgc
     5521 gcagtcagga gccggtcagg cagttaaaac cgcaggatgt ggcccgcttt tccctgcgtg
     5581 acaccatcaa ccgggtctat aaatctgcaa aggtaaaaca ccagaaaagc agcagtaaaa
     5641 aactgatcgt ctacgaagct gatggtggta cccgtgaaag cgacaaaaag ctcaaaggtg
     5701 gtaaggttac cagcgctgac tcacttaaag ttaacagccg cgtcagcgac ccggacagtg
     5761 cccggattaa agcggattca gcactggcca gacataacga ataccagcag aacggctccc
     5821 tgacgctgac gggaacacct caactgacag caggcaacaa aattgaactg gtgggttttg
     5881 ggcagttatc cgggccatgg ctcataacca ctgcccgcca tgcgtttgac cgtaacagcg
     5941 gctacaccac agagctggaa gtggcacggg ggccagtcac aagagggaaa aaacaaaaaa
     6001 ctcagaaact cacggtttat cacccggatg gcagtacatc gacggtgatt aaggagaaga
     6061 aaaaatgact ggtgtcactc gtcaggtcgg tacggtcagt gccgttgatg ccgacagggt
     6121 tcaggcccgc gttcgtctgc ctgaatgcga taacctgcgc acaaactggc ttaacgtgct
     6181 gcagcgcaat acccaggata acaaagatta ctggctccct gacgtggggg agcaggttga
     6241 ggtgctgctc gatgccaacg gcgaggatgg tgttattctg ggcgcggtgt attcagacgt
     6301 cgataaacca ccgttcagtg acaaaaatat ccggggaacc cggtttgctg atggtgcaga
     6361 gtacagctac aaccggaaga cgcacactct gatcatccgg ggcggcattg agcatattgt
     6421 cattgagtgt ggtgctgatg tggtattgaa aacacagaaa gccacgattg acgcaccgga
     6481 aaccgaactt accggagatc tgcgtgtcag gggaaagctg atttacgaag gaggcatggc
     6541 gggttctggt ggtaaaggtg ttaccgcgac catccatggc aatatcgaga ttaaagggaa
     6601 tgcccatgcc acgggcagta tgctgtctga tggcgaaaac tccagccacc actcccactg
     6661 agcttcttaa acgcctttaa tatcagcgtt cccgcacggg ggcaatactg cccccatgaa
     6721 aacgacctca gtattctggc aaccagccct gcaggctccc ggcgaaattg tccgggggct
     6781 ggatgatatc cggcaggcga ttcaaattat cctgcggact ccccgcggca gcgacccgca
     6841 tcgcccggag ttcggcagca atctgcatct ttatatcgac tggcctgtag accgggccat
     6901 tccgcatgtg gtgcgcgaat ccgtcgatgc catccggcgc tgggagcccc gctgccagct
     6961 tatgtcagtt aaacccgccg tcgacggcga acatcttacg ctccgggtga gctggaaagg
     7021 ctcagacgga cagacccgga ctcaggagct gctatggcgc tgacagaacc cgattttatt
     7081 gaacgcgatg ccgacaaaat cacggcagaa atgattgcga agtatgaagc ggataccggc
     7141 aaaacgctgt acccggcaca ggcagaacgt ctgctgattg atctgtgggc ctatcgcgaa
     7201 atgctggtca gggttgcggt acaggaggca gcaaagcaga atctggtcgc ctttgcccgt
     7261 gagccgatga ttgattacct cggtgaactg gttggtgtat accgtcttgc cgcgcagcct
     7321 gccaccacca cgctccagtt ctccgtagat gaggcactgg ccattgatgt gctgatcccg
     7381 gcaggcaccc gcgtcagcgc ttccgacagc gttatttttg ccaccgatac agatgtggta
     7441 ctgaaggccg gattgctgct ggtcaatgtc acgtccacct gtaccgaacc cggtaccgct
     7501 ggtaacggct ggcaacctgc gcaggtcagt cagttactcg atgagattga taacgtcgac
     7561 ctgctggtga gcaatctgac ggccagttcc ggcggttcag aacaggaaga cgatgacagg
     7621 ctccgggagc gtatcaggct ggccccggag tcattcacca atgccggaag ccgtggcgca
     7681 taccgctttc atgccatgca ggcccatccc aacattgtcg atgttgctgt gctttccccg
     7741 gttcccggga ccgtagatct gtatccgctg ctcagtaccg gtctgccgga cggcggtgtt
     7801 ctcacgctgg tagagagttt ctgctctgat gagaaagtca ggccactcac tgatacagtg
     7861 cgggctaaaa caccagtgaa ggtggattac accattgaag ccaggattac gatctatcgt
     7921 gatcaggatg ccaggtctgt aaaagatgcc gctaacagcg ccatacagaa ctgggtggca
     7981 tcacgtgccg ccacgctggg gcgtgatatt gtccccagcc agattatcag tgcattgtcc
     8041 gtttccgggg tgtaccaggt tgaactggtg acaccggcac tgagggtggt ggcagaaaac
     8101 gaatgggcaa actgtacggc aatcactctt aacatgactg gagtgtctga tgactgagcc
     8161 attacaactc ccgccaccgc ttgagggtga tatcagtctc aggacgctgg gaagactggc
     8221 aggacggctg gataacatcg acctgagcgt actgatggtc tttctcgtcg atatcgtcga
     8281 cagttctgcg ctgccatggc tgggcgagca gttctcactg tctggcgatg gctgggagct
     8341 ggcggaatcg gacgatgttc gccgcatgct tataaaagca gccatcgaac tgcaccggta
     8401 taaagggacg ccgtggtcaa tccgggaagt tatccgccgt tttggctttg gtgaagtgga
     8461 tctgattgaa ggcacaggtc gtctcagtta cgacggcaat cgcagctata acggactgtt
     8521 tgttcatgga gatgcagccg cctgggcagt ttatcgcgtt attctgaaac agcccattac
     8581 taacgatcag gccgcgatgc tgcgtcagac gctggctgca tttgcaccgg cccgctgcca
     8641 tctggccagc ctggagtatc agtctgtggc cattcgctat aacaataccg ccatccatga
     8701 tggcagttat aaccacggga gcagttgatt atgggaaacc tgaatgaaac agaaaagtgg
     8761 gaagaaaata tctatcaact ggagacatca gatccggttc tgggtggtgc agacg
//
LOCUS       DAPOZO010000151         8747 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPOZO010000151 DAPOZO010000000
VERSION     DAPOZO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.151"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(66..500)
                     /gene="intI1"
                     /locus_tag="ROI21_004394"
                     /pseudo
     CDS             complement(66..500)
                     /gene="intI1"
                     /locus_tag="ROI21_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
     gene            658..1131
                     /locus_tag="ROI21_004395"
     CDS             658..1131
                     /locus_tag="ROI21_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HDX5113234.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            1262..2050
                     /locus_tag="ROI21_004396"
     CDS             1262..2050
                     /locus_tag="ROI21_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HDX5113235.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            2256..2603
                     /locus_tag="ROI21_004397"
     CDS             2256..2603
                     /locus_tag="ROI21_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDX5113236.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            2597..3436
                     /gene="sul1"
                     /locus_tag="ROI21_004398"
     CDS             2597..3436
                     /gene="sul1"
                     /locus_tag="ROI21_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDX5113237.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            3564..3767
                     /locus_tag="ROI21_004399"
     CDS             3564..3767
                     /locus_tag="ROI21_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113238.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            3923..5128
                     /gene="chrA"
                     /locus_tag="ROI21_004400"
     CDS             3923..5128
                     /gene="chrA"
                     /locus_tag="ROI21_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HDX5113239.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            5139..5444
                     /locus_tag="ROI21_004401"
     CDS             5139..5444
                     /locus_tag="ROI21_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX5113240.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            5488..5553
                     /locus_tag="ROI21_004402"
                     /pseudo
     CDS             5488..5553
                     /locus_tag="ROI21_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5671..6435
                     /locus_tag="ROI21_004403"
     CDS             5671..6435
                     /locus_tag="ROI21_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HDX5113241.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(6928..7512)
                     /locus_tag="ROI21_004404"
     CDS             complement(6928..7512)
                     /locus_tag="ROI21_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDX5113242.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(7512..>8747)
                     /locus_tag="ROI21_004405"
     CDS             complement(7512..>8747)
                     /locus_tag="ROI21_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5113243.1"
                     /translation="SERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWAG
                     ATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTGL
                     IACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGAA
                     IGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLLR
                     PLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVGA
                     RISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRTP
                     PELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQF
                     ATMAAAATASAPTHTEGEH"
BASE COUNT         1853 a         2472 c         2551 g         1871 t
ORIGIN      
        1 atgtgcagct ccatcagcaa aaggggatga taagtttatc accaccgact atttgcaaca
       61 gtgcccatgc ccgttccata cagaagctgg gcgaacaaac gatgctcgcc ttccagaaaa
      121 ccgaggatgc gaaccacttc atccggggtc agcaccaccg gcaagcgccg cgacggccga
      181 ggtcttccga tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc gtagaagaac
      241 agcaaggccg ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc
      301 caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg
      361 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa actgtaatgc
      421 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
      481 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc tatgcctcgg
      541 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
      601 gatgttacgc agcagggcag tcgccctaaa acaaagttag ccattaaggg agttaaattg
      661 aaaatatcat tgatttctgc agtgtcagaa aatggcgtaa tcggtagtgg tcctgatatc
      721 ccgtggtcag taaaaggtga gcaactactc tttaaagcgc tcacatataa tcaatggctc
      781 cttgtcggaa gaaaaacatt tgactctatg ggtgttcttc caaatcgcaa atatgcagta
      841 gtgtcaaaga acggaatttc aagctcaaat gaaaacgtcc tagtttttcc ttcaatagaa
      901 aatgctttga aagagctatc aaaagttaca gatcatgtat atgtctctgg cgggggtcaa
      961 atctataata gccttattga aaaagcagat ataattcatt tgtctactgt tcacgttgaa
     1021 gtcgaaggtg atatcaaatt ccctataatg cctgagaatt tcaatttggt ttttgaacag
     1081 ttttttatgt ctaatataaa ttatacatac cagatttgga aaaaaggcta acaatgcgtt
     1141 gcagcaccag tcgcttcgct ccttggacag cttttaagtc gcgtctttgt ggttttgctg
     1201 cgcaaaagta ttccacaaag ccgcaactta aaagctgccg ctgaacttaa cgttaggcat
     1261 catgggtgaa tttttccctg cacaagtttt caagcagctg tcccacgctc gcgcggtgat
     1321 cgagcgccat ctggctgcga cactggacac aatccacctg ttcggatctg cgatcgatgg
     1381 agggctgaag ccggacagcg acatagactt gctcgtgacc gtcagcgccg cacctaacga
     1441 ttcgctccgg caggcgctaa tgctcgattt gctgaaagtc tcatcaccgc caggcgatgg
     1501 cggaacatgg cgaccgctgg agctaactgt tgtcgctcga agcgaagtag tgccttggcg
     1561 ctatccggcg cggcgtgagc ttcagttcgg tgagtggctc cgccacgaca tcctttccgg
     1621 aacgttcgag cctgccgttc tggatcacga tcttgcgatt ttgctgacca aggcgaggca
     1681 acacagcctt gcgcttctag gcccatccgc agccacgttt ttcgagccgg tgccgaagga
     1741 gcatttctcc aaggcgcttt tcgacactat tgcccagtgg aatgcagagt cggattggaa
     1801 gggtgacgag cggaacgtcg ttcttgctct tgctcgcatt tggtacagcg cttcaactgg
     1861 tctcattgct cctaaggacg ttgctgccgc atgggtatcg gagcgtttgc ctgccgagca
     1921 tcggcccctc atctgcaagg cacgcgcggc gtacctgggt agcgaggacg acgacctagc
     1981 aatgcgcgtc gaagagacgg ccgcgttcgt tcgatatgcc aaagcaacga ttgagagaat
     2041 cttgcgttga gcggcatgtg cgaaaagtgc atcgacccgc gccgagggca tctgatgcct
     2101 aactcggcgt tcaagcggac gggctgcgcc cgccgctcaa ctatgcgtta gatgcactaa
     2161 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     2221 gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta
     2281 tcgcaatagt tggcgaagta atcgcaacat ccgcattaaa atctagcgag ggctttacta
     2341 agcttgcccc ttccgccgtt gtcataatcg gttatggcat cgcattttat tttctttctc
     2401 tggttctgaa atccatccct gtcggtgttg cttatgcagt ctggtcggga ctcggcgtcg
     2461 tcataattac agccattgcc tggttgcttc atgggcaaaa gcttgatgcg tggggctttg
     2521 taggtatggg gctcataatt gctgcctttt tgctcgcccg atccccatcg tggaagtcgc
     2581 tgcggaggcc gacgccatgg tgacggtgtt cggcattctg aatctcaccg aggactcctt
     2641 cttcgatgag agccggcggc tagaccccgc cggcgctgtc accgcggcga tcgaaatgct
     2701 gcgagtcgga tcagacgtcg tggatgtcgg accggccgcc agccatccgg acgcgaggcc
     2761 tgtatcgccg gccgatgaga tcagacgtat tgcgccgctc ttagacgccc tgtccgatca
     2821 gatgcaccgt gtttcaatcg acagcttcca accggaaacc cagcgctatg cgctcaagcg
     2881 cggcgtgggc tacctgaacg atatccaagg atttcctgac cctgcgctct atcccgatat
     2941 tgctgaggcg gactgcaggc tggtggttat gcactcagcg cagcgggatg gcatcgccac
     3001 ccgcaccggt caccttcgac ccgaagacgc gctcgacgag attgtgcggt tcttcgaggc
     3061 gcgggtttcc gccttgcgac ggagcggggt cgctgccgac cggctcatcc tcgatccggg
     3121 gatgggattt ttcttgagcc ccgcaccgga aacatcgctg cacgtgctgt cgaaccttca
     3181 aaagctgaag tcggcgttgg ggcttccgct attggtctcg gtgtcgcgga aatccttctt
     3241 gggcgccacc gttggccttc ctgtaaagga tctgggtcca gcgagccttg cggcggaact
     3301 tcacgcgatc ggcaatggcg ctgactacgt ccgcacccac gcgcctggag atctgcgaag
     3361 cgcaatcacc ttctcggaaa ccctcgcgaa atttcgcagt cgcgacgcca gagaccgagg
     3421 gttagatcat gcctagcatt caccttccgg ccgcccgcta gcggaccctg gtcaggttcc
     3481 gcgaaggtgg gcgcagacat gctgggctcg tcaggatcaa actgcactat gaggcggcgg
     3541 ttcataccgc gccaggggag cgaatggaca gcgaggagcc tccgaacgtt cgggtcgcct
     3601 gctcgggtga tatcgacgag gttgtgcggc tgatgcacga cgctgcggcg tggatgtccg
     3661 ccaagggaac gcccgcctgg gacgtcgcgc ggatcgaccg gacattcgcg gagaccttcg
     3721 tcctgagatc cgagctccta gggatcgcct cagaaaacgg aaaataaagc acgctaagcc
     3781 gtaagtaagc gtgctcctgt gaaagccaca gctaaaactg cgtagtacac atagaggtat
     3841 ttttttctag atttatatcg aatttcgtta tcgaatttcg cttattattg atcaactcgt
     3901 tgcagatcat gaaggcgaaa gtatgacgaa taaccctacc gatgacagca gaccatggtc
     3961 ggtctttctt atttttctgc ggcttggatt gacatctttt ggcggcccca ttgcgcactt
     4021 gggctacttc cgcgccgaat ttgtcacacg gcggcgctgg ctctccgaac ggagctatgc
     4081 tgacttggtc gcgctttgtc agttcttgcc agggcctgca agcagccagg tcggcatagc
     4141 ggtaggactg tctcgggctg gatacagcgg ggcgctggct gcttgggctg gcttcacgct
     4201 gccgtctgcc atagccttga tcctttttgc gctcggcatc tccagctatg gcgattacgt
     4261 ctcgcagggc gcgttgcatg gcttaaaagt ggtggctgtg gccgtggtcg ctcaagcagt
     4321 atggggcatg gcgcgtaacc tatgcacgga tgggctgcga gtcaccatca tggcaattgc
     4381 tacctgcgtc gttttacttg tgccgtccgc gtggggacag gttggcgtga ttgctatcgc
     4441 aggcatcgca ggccggttat tgttcaagcc agcgaaagtt gttgagcatg accccctacc
     4501 tatcacggtc agtcaccggg ccggcgtgct ttggctctcg ctgttctttg tcttgctgat
     4561 tggcctgccg gtgttggccg aactgatgcc aagtcaaacc atggcaatgg tggattcctt
     4621 ctatcgtgtc ggatcactgg tgttcggcgg tggtcacgtt gtgctgccat tactgcaagc
     4681 cgaagtggtg ccctccggct gggtcaacaa tgaatccttt ctcgcggggt acggggcagc
     4741 tcaagcggtg cccggccctt tgttcacgtt cgccgcgttt cttggtgcct cgatgaacac
     4801 cgccccgtcg ggctggatcg gcggcattgt gtgtctgctg gctatcttcg cgccctcgtt
     4861 cttgctggtc gtcggatcaa tgccattttg ggagcgtttg cgccgcaata caggcatcca
     4921 agctgcgctg gccgggatca atgccgctgt agtcggcttg ctgctggccg cgctgtatca
     4981 gcctgtatgg actagcgcca tctttcagcc gcaagacttc ggcttggcat tagttgccct
     5041 tgtcgcactt atgttctgga agctcccgcc gtggctggtt gtcctcggta gcggtgcagc
     5101 tgggtggctg ttgagcgtcg ctctgtgagg cacaaaaaat gactgacaaa gacctctacg
     5161 gcggtttgat ccgcctgcac atccttcacc atgcagccga ggaacctgtc tttgggctgg
     5221 ggatcatcga agagctacgc cgacacggct acgagatgag cgctggcacc gtgtacccga
     5281 tgctgcacgg cctggaaaaa aaaggctatc tgacctcacg ccacgaacgc accgggcgac
     5341 gtgaacggcg tgtttatgac atcactgaac aaggcagaac tgcacttgct gatgcgaaaa
     5401 caaaggtcaa ggagctgttc ggagagttgg tagaaggtgg ttgagttttt gcttctatgt
     5461 caggttgagc tataccctaa ctggattgtc attttcagaa gacgactgca ccagttgatt
     5521 gggcgtaatg gctgttgtgc agccagctcc tgacagttca atatcagaag tgatctgcac
     5581 caatctcgac tatgctcaat actcgtgtgg gctctgttgc aaaaatcgtg aagcttgagc
     5641 atgcttggcg gagattggac ggacggaacg atgacggatt tcaagtggcg ccatttccag
     5701 ggtgatgtga tcctgtgggc ggtgcgctgg tattgtcgct atccgatcag ctatcgcgac
     5761 cttgaggaaa tgctggcgga acgcggcatt tcggtcgacc atacgacgat ctatcgctgg
     5821 gtccagtgct acgccccgga gatggagaag cggctgcgct ggttctggcg gcgtggcttt
     5881 gatccgagct ggcgcctgga tgaaacctac gtcaaggtgc ggggcaagtg gacctacctg
     5941 taccgggcag tcgacaagcg gggcgacacg atcgatttct acctgtcgcc gacccgcagc
     6001 gccaaggcag cgaagcggtt cctgggcaag gccctgcgag gcctgaagca ctgggaaaag
     6061 cctgccacgc tcaataccga caaagcgccg agctatggtg cagcgatcac cgaattgaag
     6121 cgcgaaggaa agctggaccg ggagacggcc caccggcagg tgaagtatct caataacgtg
     6181 atcgaggccg atcacggaaa gctcaagata ctgatcaagc cggtgcgcgg tttcaaatcg
     6241 atccccacgg cctatgccac gatcaaggga ttcgaagtca tgcgagccct gcgcaaagga
     6301 caggctcgcc cctggtgcct gcagcccggc atcaggggcg aggtgcgcct tgtggagaga
     6361 gcttttggca ttgggccctc ggcgctgacg gaggccatgg gcatgctcaa ccaccatttc
     6421 gcagcagccg cctgatcggc gcagagcgac agcctacctc tgactgccgc caatctttgc
     6481 aacagagccc gccgtgctag tctgctcggt gatggtggag tgaagccaac ccgcaatcgg
     6541 gttatgaatc tgcatcgcga ttcgcaatca gctgtctctt gagcatgtcg aactcctgcg
     6601 atgttatctc gccgtgctcg tgctgctaca ccaacttgtc caactcatcc gccacaccgc
     6661 tccccaccac ctgcgatggc caggttgttt gctgggcgca ttggcggagt atcgtctcga
     6721 cccgggacac ccacaccggc acaaccttac gggagtgatt cactgtcaaa gaatcggccc
     6781 ggtgctctga cgcaagtatc gggatggtca ccatttgtaa gccgtagacc tgagtggtga
     6841 tcaagacttc gataccaccg accgtaccgg tactaatcga cgacggtcgt gttcgtcgcc
     6901 tgccgcaggg actctgcaca cctccgttta cgcatgtgcc tggaggagtt ggaaatcgtc
     6961 gtgttcggga aacattaaac acaggatggc agcgatctga gccagcacat gatcagctag
     7021 ctcaccatcc ggatcgacgg cccactgcat cgtcgcgcca gcgatgaccg agtgcaggag
     7081 caactcagct gccgcaggag cacctggggg cagtcgcttg cggatcccct ccaccaccgc
     7141 gcggttccgc tggatcgcaa gcgtgcgtag ctccggcacc tggagctcgt accaggagat
     7201 gagatagttc accgagaagt cgttgcgagt gttcatgctc cgaacgagca cctgcaaaaa
     7261 ttcccagagc ccttgcggcc ctgcgcctat cggtatcgca ttcaggtaat gccgcacctg
     7321 ctcgacgccg cgctccatca tcctcaccag cagcgtatcg cggttggtga agcgctggat
     7381 taacgctgcg cgggagagcc ccacctcctt tgctactccg ctgagcgtga actctatggg
     7441 accgcaacgc ttcagcacta cggtggcggc ctcgagtacc tcgtcatcgg acttgagctt
     7501 ggggcggggc atcagtgttc accttctgta tgggttgggg cggaggctgt ggctgccgcc
     7561 gccattgtag caaattgaag acggagcgag agtagagcca cgagccccgc aaacacggcc
     7621 gatacaacga ggccagggag cggaccagca aggtcgacaa acgcgccggc cgcaagcata
     7681 accatgggcg aggctgacag catcaccgcc gagaccgtgc cgagtacccg gccgagaagt
     7741 tctggcggcg tgcggttgta gatggcagcg ttgagaatgg gagagactga gccggtcagc
     7801 agtcccacga gcgcgcccaa caacatcagc accggcacgc ctggcaactg tgaaagcaga
     7861 agcgagccca ccgcagagcc acaaaatgcc accgccagcc agttctgcgc tgatatccgg
     7921 gcgccgaccg acgcatgaat ggcaatgcca aggagaccac cagcccccat cattgaggag
     7981 aacagcccga gctctgctac ttggcgtcct gcatctacaa acagcgcagg catgatgacg
     8041 ctgccgttgg cgccaacgat gcccacgaag atcatcacta taccaaagag agggcgcagc
     8101 aggggttcgc tccagagaaa agcgacgccg gcgcgcatgg agagagtcgc cgtcgtggtc
     8161 atcgtccgag cggcacgcgc gggaagcacc cacgcgccga gcagacctgc aaggacggag
     8221 cagaacgccg tcagcccgag cgttggcgca gcgccaagca ggccgattgc ggccccccca
     8281 agggccgggc cacctagaat cgcgacgttc ccgatcaccg ctttcagtga cgagacgcgc
     8341 tcaacggaga gcccggcgac gtggccgagt ttgggcagct cactgtcctg cgcggccata
     8401 ccgggtgcgt cgaacgcggc accgagcacc acgcaagcga tcagcccagt gttcgagagg
     8461 gcgccaacgg catcgagcag tgggatgctc gccatggcca cgccgcccac cacacccgag
     8521 atcaatgcga cgggcgcgcg cccgaaccga tcgacgaggc caccaccaac ccacgcgccg
     8581 atgatggtcg cgatgacgct gctagcggcc gtggcgcccg cccaggccgc gctctttgta
     8641 tgagacagga cgaaccatgg aagcgcgagg gccgccaccg cgttgccgat ccggaagaga
     8701 aaggtcgccg cgaacagcgt cgcgagcggg ctatatcgac gttcgct
//
LOCUS       DAPOZO010000152         8690 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPOZO010000152 DAPOZO010000000
VERSION     DAPOZO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.152"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            233..754
                     /gene="fecI"
                     /locus_tag="ROI21_004406"
     CDS             233..754
                     /gene="fecI"
                     /locus_tag="ROI21_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HDX5113244.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            751..1704
                     /gene="fecR"
                     /locus_tag="ROI21_004407"
     CDS             751..1704
                     /gene="fecR"
                     /locus_tag="ROI21_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HDX5113245.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSTSEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1791..4115
                     /gene="fecA"
                     /locus_tag="ROI21_004408"
     CDS             1791..4115
                     /gene="fecA"
                     /locus_tag="ROI21_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HDX5113246.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLTSRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4160..5062
                     /gene="fecB"
                     /locus_tag="ROI21_004409"
     CDS             4160..5062
                     /gene="fecB"
                     /locus_tag="ROI21_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HDX5113247.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5059..6057
                     /gene="fecC"
                     /locus_tag="ROI21_004410"
     CDS             5059..6057
                     /gene="fecC"
                     /locus_tag="ROI21_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HDX5113248.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6054..7010
                     /gene="fecD"
                     /locus_tag="ROI21_004411"
     CDS             6054..7010
                     /gene="fecD"
                     /locus_tag="ROI21_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HDX5113249.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHHRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7011..7778
                     /gene="fecE"
                     /locus_tag="ROI21_004412"
     CDS             7011..7778
                     /gene="fecE"
                     /locus_tag="ROI21_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HDX5113250.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8335..8592)
                     /locus_tag="ROI21_004413"
     CDS             complement(8335..8592)
                     /locus_tag="ROI21_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113251.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
BASE COUNT         1894 a         2485 c         2419 g         1892 t
ORIGIN      
        1 tccccatcct ttagggcagt gaggatgtca ataatagcag accacatcat gagtcattct
       61 gaccgacaca tgccagaagc agaggctcca tcccatttta tacctacctt ataacactta
      121 gaaaaacaac atgttaaaaa tgtctattgg aaacaatttt atttccaatt gtaatgataa
      181 ccattctcat attaatatga ctacgtgata attaactttt gatgcactcc gcatgtctga
      241 ccgcgccact accacagctt ccttaacgtt cgagtcgctt tatggcacac atcacggctg
      301 gttgaaaagc tggctgacgc gcaaactcca gtctgctttt gatgcagatg acattgccca
      361 ggacactttt ttgcgggtaa tggtcagcga aacgctctcg acgatccgcg atcctcgctc
      421 cttcctctgc actatcgcca aacgcgtgat ggtggacctg tttcgccgaa acgcgctgga
      481 aaaagcgtat ctggagatgc tggcgcttat gccggagggg ggagcgcctt cacctgagga
      541 acgcgaaagc caactcgaga ccctacaact cctcgacagc atgctggacg ggctaaacgg
      601 caaaacacgt gaagcgtttc tgctttcgca actggatggt ctgacataca gcgagattgc
      661 gcacaaactc ggtgtttcca tcagctccgt gaaaaaatac gtggcgaaag ccgtcgagca
      721 ctgcctgctg ttccgtctgg agtatgggtt atgaatcctt tgttaaccga ttcccgccgt
      781 caggcgctgc gttcagcttc ccactggtat gccgtgctaa gcggcgagcg cgtcagccca
      841 caacaggaag cgcgctggca acagtggtat gaacaggatc aggataacca gtgggcctgg
      901 cagcaggttg aaaacctgcg caaccagctt ggcggtgtgc ctggcgacgt tgccagccgg
      961 gcgttgcacg atacccgcct cacccgccgt cacgtgatga aaggattact gctgttgctc
     1021 ggcgctggcg gaggctggca gctctggcag tcggaaaccg gcgaaggtct gcgggcagat
     1081 taccgcaccg ccaaaggcac agtcagccgt cagcaactgg aagatggctc cctgctcacg
     1141 ctgaataccc aaagcgcggc ggatgtgcgt tttgatgcgc atcagcgcac cgtccggctc
     1201 tggtacggtg aaatcgccat tactaccgcg aaagatgccc tgcaacgccc cttccgtgtc
     1261 ctgacccgtc agggccagct cactgcttta gggacagaat ttaccgtccg ccagcaggat
     1321 aatttcacgc agcttgacgt tcagcagcac gctgtggaag tgcttctcgc cagtgccccc
     1381 gcgcaaaaac gcatcgtgaa cgctggtgaa agcctgcagt tcagcacctc tgagtttggc
     1441 gcagtgaaac cgctggatga cgagagtaca agctggacga aggacatcct gagcttcagc
     1501 gataaaccac tgggtgaggt gatagccacg ctaacccgtt accgcaacgg cgtgctgcgc
     1561 tgcgatcccg ccgttgccgg gctgcgcctg agcgggacgt tcccgctgaa aaataccgat
     1621 gcgatcctga acgttatcgc gcaaacgctt cccgttaaaa ttcagtctat tacgcggtac
     1681 tggataaaca tttcaccact gtaaggaaaa taattcttat ttcgattgtc ctttttaccc
     1741 ttctcgttcg actcatagct gaacacaaca aaaatgatga tggggaaggt atgacgccgt
     1801 tacgcgtttt tcgtaaaaca acacctttgg ttaacgccat tcgcctgagc ctgctgccgc
     1861 tggccggtct ctcgttttcc gcttttgctg cacaggttaa tatcgcaccg ggatcgctcg
     1921 ataaagcgct caatcagtat gccgcacaca gcggatttac cctctcggtt gacgccagcc
     1981 tgacgcgcgg caagcagagc aacggcctgc acggcgatta cgacgtcgag agcggcctgc
     2041 aacaactgct ggacggcagc ggactgcagg taaaaccgct gggaaataat agctggacgc
     2101 tggagcccgc gcccgcacca aaagaagatg ccctgaccgt ggtcggcgac tggctgggtg
     2161 atgcgcgtga aaacgacgta tttgaacatg ctggcgcgcg tgacgtgatc cgccgtgagg
     2221 atttcgccaa aaccggcgca accaccatgc gtgaggtact taaccgcatc cctggcgtca
     2281 gcgcgccgga aaacaacggc accggcagcc acgacctggc gatgaacttt ggcatccggg
     2341 gcctgaaccc gcgcctcacc agccgctcga ccgtcctgat ggacggcatc cccgtcccct
     2401 tcgcccctta cggtcagccg cagctttcac tggctcccgt ttcgctcggc aacatggatg
     2461 ccattgacgt ggtacgcggt ggtggtgcgg tgcgttacgg accgcagagc gtgggcggcg
     2521 tggtgaactt tgttacccgt gccattccgc aggactttgg tatcgaggcg ggcgtggaag
     2581 gtcagctcag cccaacctct tcacaaaaca acccgaaaga gacgcacaac ctgatggtgg
     2641 gcggcacagc ggacaacggt tttggcaccg cgctgctcta ctccggcacg cgcggcagtg
     2701 actggcgcga gcacagcgcc acccgcatcg acgacctgat gctgaaaagc aaatatgcgc
     2761 cggatgaggt gcacaccttc aacagcctgc tgcaatatta cgacggtgaa gccgacatgc
     2821 ccggtggcct gtctcgcgcg gattacgacg ccgatcgctg gcaatccacc cgcccgtatg
     2881 accgcttctg gggtcgtcgc aagctggcga gcctgggcta ccagttccag ccagacagcc
     2941 agcataaatt caacattcag gggttctaca cccaaaccct gcgcagcggc tacctggagc
     3001 aaggcaaacg catcaccctc tcgccgcgta actactgggt gcgcggtatt gagccacgct
     3061 acagccagat ctttatgatc ggcccttccg cgcacgaagt gggcgtgggc tatcgctatg
     3121 tgaatgaatc aacgcatgaa atgcgttact acaccgccac cagcagcggg cagttgccgt
     3181 ccggctcaag cccttacgac cgcgatacgc gttccggcac cgaggcgcac gcctggtatc
     3241 tggatgacaa aatcgacatc ggcaactgga ccatcacgcc gggtatgcgt ttcgaacata
     3301 tcgagtcata ccagaacaac gccatcacag gcacgcacga agaagtgagc tataacgcac
     3361 cgcttccggc gttgaacgtg ctctatcacc tgactgacag ctggaatctt tatgcaaaca
     3421 ctgaaggctc gttcggcacc gtacagtaca gccagattgg caaggctgtg caaagcggca
     3481 atgtggaacc ggaaaaagcg cgaacctggg aactcggtac ccgctacgac gacggcgcgc
     3541 tgacggcgga aatggggctg ttcctgatta actttaacaa tcagtacgac tccaaccaga
     3601 ccaacgacac cgtcactgca cgtggcaaaa cgcgccatac agggctggaa acgcaggcac
     3661 gttacgatct gggtacgcta acgccaacgc ttgataacgt ttccatctac gccagctatg
     3721 cgtatgtgaa cgcggaaatc cgcgagaaag gcgacaccta cggcaatctg gtaccattct
     3781 ccccgaaaca taaaggcacg ctgggcgtgg actacaagcc aggaaactgg acgttcaatc
     3841 tgaacagcga tttccagtcc agccagtttg cggataacgc caatacggtg aaagagagcg
     3901 ccgacggcag taccggccgc attcccggct tcatgctctg gggcgcacgc gtggcgtatg
     3961 actttggccc gcagatggca gatctgaacc tggcattcgg tgtgaaaaac atcttcgacc
     4021 aggactactt catccgctct tatgacgaca acaacaaagg catctatgca ggccagccgc
     4081 gcacgctgta tatgcagggg tcgttgaagt tctgatgtgt taacgcccgg cttgccgggc
     4141 ttttagctgg aatgtgatta tgttggcatt tatccgtttt gtttttgcag gcctgctgct
     4201 ggtgatcagc cacgcctttg ccgccacggt tcaggacgaa cacggcacgt ttacactcga
     4261 aaaaacgcca caacggattg tggtgctgga actctcgttc gccgatgcgc tggccgccgt
     4321 ggacgtcagc ccgatcggta ttgccgacga taacgatgca aaacgcatcc tgcccgaagt
     4381 gcgtgcgcac ctgaaaccgt ggcagtccgt cggaacgcgc gcgcagccga gcctggaagc
     4441 cattgccgct ctgaaaccag acctgatcat tgccgacagc agtcgccatg cgggggttta
     4501 catcgccttg cagcaaatcg cgccggtact gctgcttaag tcccgcaacg aaacctacgc
     4561 tgaaaatttg caatctgcgg ctatcatcgg cgaaatggtg ggtaaaaagc gagagatgca
     4621 ggcacgcctg gaacaacata aagagaggat ggcgcagtgg gccagccagc ttcccaaagg
     4681 gacacgcgtg gcctttggca catcacgcga acagcaattc aacctgcata ctcaggagac
     4741 ctggaccggc agcgtgctgg cctctctggg gctgaacgtt cccgctgcga tggcgggtgc
     4801 gtccatgccg tccatcggcc tggagcaact gctggcggtc aatcctgcct ggctgctggt
     4861 tgcccactat cgcgaagaga gcattgttaa acgctggcaa caagatccgc tctggcagat
     4921 gttaaccgcc gcgcagaagc agcaggttgc ttcggtcgac agtaacacct gggcgcggat
     4981 gcgcggtatt tttgctgcag agcgtattgc cgctgacacg gtaaaaatct tccaccatca
     5041 gccgcttacc gttgtgaaat gaccgcgata aaacacccgg tgctgctgtg ggggcttccc
     5101 gttgcagcac ttattattat tttctggctg agtctgtttt gctactcggc cattcctgtt
     5161 tccggagcag atgcaacccg cgccctgctg cctggacaca cgccaacgct accagaagcg
     5221 ctggtgcaaa accttcgttt gccacgaagc ctggtcgccg ttctgatcgg cgcaagcctg
     5281 gcgctcgcgg gcacgctgct gcaaaccctg acccacaacc caatggcctc tccttcactg
     5341 ctcggcatta acagcggcgc ggcgctggct atggcgctta ccagcgcgct gagtccgacg
     5401 ccgattgcag gctattctct gtcgttcatc gcggcatgcg ggggcggcgt gagctggctg
     5461 ctggtcatga ccgcaggagg cggatttcgc catacccatg acagaaacaa actgatcctc
     5521 gcgggtatcg cgctgtcggc cttttgtatg ggccttaccc gcatcaccct gctgctggcc
     5581 gaagatcatg cttacggcat cttttactgg ctggcaggcg gagtgtccca cgcccgctgg
     5641 caggatgtct ggcagctctt gccggtggtg gtcactgcag tccctgtcgt gttgctgctg
     5701 gcgaatcaac tgaacctgct caacctcagc gacagcaccg cccatacgct gggagtgaac
     5761 ctgacgaggc tacgtttggt catcaatatg ttagtgctgc ttctggttgg tgcgtgcgtc
     5821 agcgtggcag gtccggtggc gtttatcggt ctgctggtgc cacatctggc gcgcttctgg
     5881 gcaggcttcg atcagcgcaa cgtactgcca gtgagcatgc tgctgggggc cacgctgatg
     5941 ctgctggcag atgtactcgc acgcgcactg gccttccccg gagatctgcc cgcaggcgca
     6001 gtgctggcgc tgattggcag cccttgcttt gtctggcttg tgaggaggcg aggatgaaaa
     6061 ttgcgctggt tattttcatc acccttgccc tggcgggctg tgcgctgtta tcactccata
     6121 tgggagtgat ccccgtgccg tggcgcgcgc tgctgaccga ctggcaggcc ggacgcgagc
     6181 attattatgt attgatggag taccgactgc cgcgcttgct gctggcactg tttgtcggtg
     6241 cagccctcgc cgtggcgggc gtgctgatac aggggattgt gcgcaaccct ctggcatcac
     6301 cggatattct cggtgttaac catgccgcca gcctggcctc tgtgggggct ctacttctta
     6361 tgccgtcact gcccgtgatg gtgctgccgc tgctggcctt tgcgggcggc atggcggggt
     6421 tgatattgct gaagatgctg gcaaagaccc accagccgat gaagctggcg ctcaccggcg
     6481 tggcgctttc tgcatgctgg gccagcctga cggattatct gatgctctcg cgcccgcagg
     6541 atgtgaacaa cgccctgctg tggctgaccg gcagcttatg gggccgtgac tggagctttg
     6601 tgaagattgc catcccgctg atgattttat ttctgccgct gagcctgagt ttttgccgcg
     6661 atctcgacct ccttgcactc ggcgatgcgc gcgccaccac gctcggtgtg tcggtgcccc
     6721 atacccgatt ctgggctttg ttactagctg tcgccatgac atctaccggc gtggccgcct
     6781 gcggcccgat tagctttatt ggtctcgtgg tgccgcatat gatgcgtagc atcaccggtg
     6841 gacgtcacca cagactgctg cctgtttcgg ccctgacagg tgcgttgctg ttggtggttg
     6901 ccgatctgct ggcgagaatt attcatcccc cactggagct cccggttggc gtgctgaccg
     6961 ccattatcgg tgcgccgtgg tttgtctggt tgcttgtgag aatgcgataa atgactttac
     7021 gaactgaaaa tctgacggtc agttacggga cagacaaggt acttaacgac gtttcactct
     7081 cactgccaac ggggaagatc accgccctga tcggtcctaa cggttgcggg aaatcgacgc
     7141 tgttaaactg tttttcgcgg cttttaatgc cgcagtctgg caccgtattt ctcggcgata
     7201 atcccataaa tatgctctca tcgcgccagt tggcccgcag gctttcgctg ctgcctcagc
     7261 accatttaac gccagagggg atcacagtcc aggagctggt ttcgtatggt cgtaatccct
     7321 ggctgtcact ctgggggcgt ctctccgctg aagacaatgc acgagttaat gtcgccatga
     7381 accagacccg gatcaatcat cttgccgttc gtcggttaac cgagctttcc ggcggtcagc
     7441 gccagcgcgc atttctggca atggtcctgg cccagaatac gcccgttgta ttacttgatg
     7501 agccaaccac ctatcttgat atcaatcacc aggtggacct gatgcggttg atgggcgaac
     7561 tccggactca ggggaaaacg gtggtcgctg tgctgcacga ccttaatcag gctagccggt
     7621 actgcgatca actggtggta atggcaaacg gacatgttat ggcgcaaggc acaccagaag
     7681 aggtgatgac cccaggattg ctgagaacag tattcagcgt ggaagcggaa atacaccccg
     7741 agccggtatc tggcaggccg atgtgcctaa tgaggtagat tgcacaggcc gtaagaacca
     7801 aaccacgact gaatgaaact ggactggcgc cagcaagcct gttcagactg gggctgaact
     7861 tttccggact ctgaaagatt accaatactc atcgtccatc cgcttgcttt aggctgacag
     7921 gttcataatc aacgcaaacc agagctgtac aggcttgggc gcggctttca aaccagtcgt
     7981 gatcacggca atcaattttg aactctgctt aacggacatt tctgtataac ccttacggca
     8041 acgaaaaacg cgaagttaaa attttagaaa cccaaaaacg tgacatgact aagtttagat
     8101 ttcagggggg agatcaaaaa atttcgctct gtgccagagc ggacattcac ggagctggtt
     8161 cattaccaat gaggttgggc ttttgaggat aaatcaatga tcagacgcca acgtaaatca
     8221 aaagcaaccc tggaacggaa ttgctaatcc agtttctgac catcgatttt tctaaaaagt
     8281 gtgcgtttgc tactacttag gtaggtgcag ctttcttaat caccggcagc cacgttatac
     8341 aggccagttg atggatcgat tgttatcaat gatatcttca tgagtcggtg tctcacccag
     8401 cttaccgaag ctggcataaa gtgaaggcag acgggcccgt ccttctccct ttttcgccag
     8461 agggaaagcg cgaagcatgg tggcagcctc ccagtcacaa caataggatg gtgtgcacgg
     8521 ctgctgacgc catgattcag cgatagagcc ggaaaatacg gggtcaaagc cggtatcatt
     8581 aaccagagtc atcgtgacct gtactgcggc tggatgatct cctgctactg caactgccag
     8641 gcgaccgcgg ctcccttcag gtagtctgtt gtggtcaact aaaactggcc
//
LOCUS       DAPOZO010000153         8544 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPOZO010000153 DAPOZO010000000
VERSION     DAPOZO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.153"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(254..1225)
                     /locus_tag="ROI21_004414"
     CDS             complement(254..1225)
                     /locus_tag="ROI21_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HDX5113252.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDSLDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(1225..2391)
                     /gene="sopA"
                     /locus_tag="ROI21_004415"
     CDS             complement(1225..2391)
                     /gene="sopA"
                     /locus_tag="ROI21_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HDX5113253.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(2979..3734)
                     /gene="repE"
                     /locus_tag="ROI21_004416"
     CDS             complement(2979..3734)
                     /gene="repE"
                     /locus_tag="ROI21_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HDX5113254.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(4454..5260)
                     /locus_tag="ROI21_004417"
     CDS             complement(4454..5260)
                     /locus_tag="ROI21_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDX5113255.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(5261..5566)
                     /gene="ccdB"
                     /locus_tag="ROI21_004418"
     CDS             complement(5261..5566)
                     /gene="ccdB"
                     /locus_tag="ROI21_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HDX5113256.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(5568..5786)
                     /gene="ccdA"
                     /locus_tag="ROI21_004419"
     CDS             complement(5568..5786)
                     /gene="ccdA"
                     /locus_tag="ROI21_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HDX5113257.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            6494..7489
                     /locus_tag="ROI21_004420"
     CDS             6494..7489
                     /locus_tag="ROI21_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDX5113258.1"
                     /translation="MATTVIAAFNEFMKDTVNLKKADTDDARASRDWLIGKMNDFEKD
                     DKFPVSFPAIHIAFGSFARRTKIRPLDDIDLMFGLTGQGATYTILSDRITVTSSGEGS
                     RLHSYRHSGADTVCSVRILNAFKNRLQDIAQYAQADIRRNQEAVTLKLVSKDWNFDIV
                     PCFITSEDAFGRTYYLIPDGNGHWKFTDPRKDRDRVTTINVQNNGNVLNVIRAVKYWQ
                     RRPTMPSMSSYLLETLILDYYAGRTSCSSFVDMELEALFRHLGQSVRYSVNDPKGIQG
                     DINSLSAEARKAISDRCYLDAQKVSEARWFENNKEYEKSINKWRDVFGPFFPVYG"
     gene            7493..8425
                     /locus_tag="ROI21_004421"
     CDS             7493..8425
                     /locus_tag="ROI21_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113259.1"
                     /translation="MNAIYTNQSTRENMDLLYAQARVYDRVKNWNRLNFLFSIIVPLL
                     LSFVTVYNRSREFVDSELLSSLLGLYGLLVLTFNIVISGHISALRRKAASIQEMYDCR
                     VLGIRRNELKVEEIPRDDIIREAAYFRDSPEKARKRFGEEGWYVSKVYDAPQAVMALL
                     CHGKNLGWDKSLREVLHVFYLSAFIVSPVAMLVYGIVMKSGLNEILFYVVFTLPVIRY
                     FLLQFLDNRSSMKRSEKLKKYVEKELSGIRVSGRAEEEQLAYTLRNIQDEMFTYRASC
                     PPVPNGIQLIMKPKNERIYVDYFETNLKELHLQK"
BASE COUNT         2343 a         2093 c         1951 g         2157 t
ORIGIN      
        1 atcagaccga cgatacgagt gggaccatgg tcccagacta ataatcagac cgacgatacg
       61 agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc
      121 agtctgatta tcagaccgac gatacaagtg gaacagtggg cccagagaga atattcaggc
      181 cagttatgct ttctggcctg taacaaagga cattaagtaa agacagataa acgtagacta
      241 aaacgtggtc gcatcagggt gctggctttt caagttcctt aagaatggcc tcaattttct
      301 ctatacactc agttggaaca cgagacctgt ccaggttaag caccatttta tcgcccttat
      361 acaatactgt cgctccagga gcaaactgat gtcgtgagct taaactagtt cttgatgcag
      421 atgacgtttt aagcacagaa gttaaaagag tgataacttc ttcagcttca aatatcaccc
      481 cagctttttt ctgctcatga aggttagatg cctgctgctt aagtaattcc tctttatctg
      541 taaaggcttt ttgaagtgca tcacctgacc gggcagatag ttcaccgggg tgagaaaaaa
      601 gagcaacaac tgatttaggc aatttggctg tgttgataca gcgggtaata atcttacgtg
      661 aaatattttc cgcatcagcc agcgcagaaa tatttccagc aaattcattc tgcaatcggc
      721 ttgcataacg ctgaccacgt tcataagcac ttgttgggcg ataatcgtta cccaatctgg
      781 ataatgcagc catctgctca tcatccagct cgccaaccag aacacgataa tcactttcgg
      841 taagtgcagc agctttacga cggcgactcc catcggcaat ttctatgaca ccagatactc
      901 ttcgaccgaa cgccggtgtc tgttgaccag tcagtagaaa agaagggatg agatcatcca
      961 gtgagtcctc agtaagcagc tcctggtcac gttcattacc tgaccatacc cgagaggtct
     1021 tctcaacact atcaccccgg agcacttcaa gagtaaactt cacatcccga ccacatacag
     1081 gcaaagtaat ggcattaccg cgagccatta ctcctacgcg cgcaattaac gaatccacca
     1141 tcggggcagc tggtgtcgat aacgaagtat ctccaaccgg ttgagtattg agcgtatgtt
     1201 ttggaataac aggcgcacgc ttcattatct aatctcccag cgtggtttaa tcagacgatc
     1261 gaaaatttca ttgcagacag gttcccaaat agaaagagca tttctccagg caccagttga
     1321 agagcgttga tcaatggcct gttcaaaaac agttctcatc cggatctgac ctttaccaac
     1381 ttcatccgtt tcacgtacaa cattttttag aaccatgctt ccccaggcat cccgaatttg
     1441 ctcctccatc cacggggact gagagccatt actattgctg tatttggtaa gcaaaatacg
     1501 tacatcaggc tcgaaccctt taagatcaac gttcttgagc agatcacgaa gcatatcgaa
     1561 aaactgcagt gcggaggtgt agtcaaacaa ctcagcaggc gtgggaacaa tcagcacatc
     1621 agcagcacat acgacattaa tcgtgccgat acccaggtta ggcgcgctgt caataactat
     1681 gacatcatag tcatgagcaa cagtttcaat ggccagtcgg agcatcaggt gtggatcggt
     1741 gggcagttta ccttcatcaa atttgcccat taactcagtt tcaatacggt gcagagccag
     1801 acaggaagga ataatgtcaa gcccaggcca gcaagtgggc tttattgcat aagtgacatc
     1861 gtccttttcc ccaagataga aaggcaggag agtgtcttct gcatgaatat gaagatctgg
     1921 tacccatccg tgatacattg aggctgttcc ctgggggtcg ttaccttcca cgagcaaaac
     1981 acgtagcccc ttcagagcca gatcctgagc aagatgaaca gaaactgagg ttttgtaaac
     2041 gccaccttta tgggcagcaa ccccgatcac cggtggaaat acgtcttcag cacgtcgcaa
     2101 tcgcgtacca aacacatcac gcatatgatt aatttgttca attgtataac caacacgttg
     2161 ctcaacccgt cctcgaattt ccatatccgg gtgcggtagt cgccctgctt tctcggcatc
     2221 tctgatagcc tgagaagaaa ccccaactaa atccgctgct tcacctattc tccagcgccg
     2281 ggttattttc ctcgcttccg ggctgtcatc attaaactgt gcaatggcga tagccttcgt
     2341 catttcatga ccagcgttta tgcactggtt aagtgtttcc atgagtttca ttctgaacat
     2401 cctttaatca ttgctttgcg tttttttatt aaatcttgca atttactgca aagcaacaac
     2461 aaaatcgcaa agtcatcaaa aaaccgcaaa gttgtttaaa ataagagcaa cactacaaaa
     2521 ggagataaga agagcacata cctcagtcac ttattatcac tagcgctcgc cgcagccgtg
     2581 taaccgagca tagcgagcga actggcgagg aagcaaagaa gaactgttct gtcagatagc
     2641 tcttacgctc agcgcaagaa gaaatatcca ccgtgggaaa aactccaggt agaggtacac
     2701 acgcggatag ccaattcaga gtaataaact gtgataatca accctcatca atgatgacga
     2761 actaaccccc gatatcaggt cacatgacga agggaaagag aaggaaatca actgtgacaa
     2821 actgccctca aatttggctt ccttaaaaat tacagttcaa aaagtatgag aaaatccatg
     2881 caggctgaag gaaacagcaa aactgtgaca aattaccctc agtaggtcag aacaaatgtg
     2941 acgaaccacc ctcaaatctg tgacagataa ccctcagact atcctgtcgt catggaagtg
     3001 atatcgcgga aggaaaatac gatatgagtc gtctggcggc ctttcttttt ctcaatgtat
     3061 gagaggcgca ttggagttct gctgttgatc tcattaacac agacctgcag gaagcggcgg
     3121 cggaagtcag gcatacgctg gtaactttga ggcagctggt aacgctctat gatccagtcg
     3181 attttcagag agacgatgcc tgagccatcc ggcttacgat actgacacag ggattcgtat
     3241 aaacgcatgg catacggatt ggtgatttct tttgtttcac taagccgaaa ctgcgtaaac
     3301 cggttctgta acccgataaa gaagggaatg agatatgggt tgatatgtac actgtaaagc
     3361 cctctggatg gactgtgcgc acgtttgata aaccagggaa aagattcata gcctttttca
     3421 tcgccggcat cctcttcagg gcgataaaaa accacttcct tccccgcgaa actcttcaat
     3481 gcctgccgta tatccttact ggcttccgca gaggtcaatc cgaatatttc agcatattta
     3541 gcaacatgga tctcgcagat accgtcatgt tcctgtaggg tgccatcaga ttttctgatc
     3601 tggtcaacga acagatacag catacgtttt tgatcccggg agagactata tgccgcctca
     3661 gtgaggtcgt ttgactggac gattcgcggg ctatttttac gtttcttgtg attgataacc
     3721 gctgtttccg ccatgacaga tccatgtgaa gtgtgacaag tttttagatt gtcacactaa
     3781 ataaaaaaga gtcaataagc agggataact ttgtgaaaaa acagcttctt ctgagggcaa
     3841 tttgtcacag ggttaagggc aatttgtcac aacaccttct ctagaaccag catggataaa
     3901 ggcctacaag gcgctctaaa aaagaagatc taaaaactat aaaaaaaata attataaaaa
     3961 tatccccgtg gataagtgga taaccccaag ggaagttttt tcaggcatcg tgtgtaagca
     4021 gaatatataa gtgctgttcc ctggtgcttc ctcgctcact cgagggcttc gccctgtcgc
     4081 tcgactgcgg cgagcactac tggctgtaaa aggacagacc acatcatggt tctgtgttca
     4141 ttaggttgtt ctgtccattg ctgacataat ccgctccact tcaacgtaac accacacgaa
     4201 gatttctatt gttcctgaag gcatattcaa atcgttttcg ttaccgcttg caggcatcat
     4261 gacagaacac tacttcctat aaacgctaca caggctcctg agattaataa tgcggatctc
     4321 tacgataatg ggagattttc ccgactgttt cgttcgcttc tcagtggata acagccagct
     4381 tctctgttta acagacaaaa acagcatatc cactcagttc cacatttcca tataaaggcc
     4441 aaggcattta ttctcaggat aattgtttca gcatcgcaac cgcatcagac tccggcatcg
     4501 caaactgcac ccggtgccgg gcagccacat ccagcgcaaa aaccttcgtg tagacttccg
     4561 ttgaactgat ggacttatgt cccatcaggc tttgcagaac tttcagcggt ataccggcat
     4621 acagcatgtg catcgcatag gaatggcgga acgtatgtgg tgtgaccgga acagagaacg
     4681 tcacaccgtc agcagcagcg gcggcaaccg cctccccaat ccaggtcctg accgttctgt
     4741 ccgtcacttc ccagatccgc gctttctctg tccttcctgt gcgacggtta cgccgctcca
     4801 tgggtatttt cagtgttgcc accatcgtct gcagctggct gacgtaccag gagtcagaga
     4861 gcggaaccag ccggtgagtc tgctgaccgg cgggcattct ccccgccgtc ctggcagctt
     4921 tttcggtccg ttgtttcagg gtcgcaagct gcacaaacgg atacggaggc gcaagcgaaa
     4981 aatccccccg cgtcagcgcc agtgcttcat taatgcgtgc tccggtgttc cacagtgtgg
     5041 ccagcagcat cttgcggtgc agatccggga cgtaatggag cagggcactc acttccggag
     5101 ccagcagata ttttggcagt tcatcatgga ccatcgacat ctggcgaagt gccagagctg
     5161 ccggataatc aatggcaaca ggcagcgatg caggctgccc ggcagaatac actgccggta
     5221 ccatgactgc agactggctg tgtataacgg agcctgacat ttatattccc cagaacatca
     5281 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
     5341 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
     5401 tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca
     5461 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
     5521 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagtccctg
     5581 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
     5641 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg
     5701 cttaccagac cggagatatt gacatcatat gccttgagca actgatagct gtcgctgtca
     5761 actgtcactg taatacgctg cttcatagca cacctctttt tgacatactt cgggtataca
     5821 tatcagtata tattcttatg ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg
     5881 cttttagtaa gccttatgta ttttaccttt cgttatgtta accaataaaa attaaaatct
     5941 gacttataaa aacaaagcgt aattaccgca ttcccgtttc gtatggatcc gactttattt
     6001 gaataaacga ctttccggaa gtgtacaacg ctgagaaaat catcatatcg ctgttttccg
     6061 cccctgcgcg gcgcatcaca gtccagagat acaacacaac atatagtgcc attggcaact
     6121 gtatgaggca ctacatgctg tgctgacggg atgtaacgct gtgcggaggg agaggaagat
     6181 ccgtaactta cttttttcgg atctctggag aggggaagaa ggatttgtgc tgatgatgca
     6241 atactaatga tatattttga acttaattgc attattgctt atctggcatt aataactgaa
     6301 atactttgca taattatcaa tgaaagaacg aggctcatat ttcccatgta aattatatgc
     6361 ccttgcagag agcgagacgg tagtgaactt gcggcactaa taataaactg taccgactct
     6421 tcccgggcac tggaatgttc agatgtacga cagagccccg cgccttttgc aataaataat
     6481 taaaggactg aacatggcga ccacagtaat agctgccttt aacgaattta tgaaagatac
     6541 cgtgaatctc aaaaaggcag ataccgatga cgcgcgtgca agtcgcgact ggcttatcgg
     6601 taagatgaat gattttgaga aggacgataa atttccggtg agttttccag cgatccatat
     6661 tgcctttggc tcctttgcca gaagaaccaa aatccgtccg cttgatgata tcgatctgat
     6721 gtttggctta accgggcaag gtgccaccta taccatcctg agtgaccgga taactgtaac
     6781 ctcctccgga gaggggtcac gtctgcacag ttaccgccat tccggagctg acaccgtctg
     6841 ctccgtcaga attctgaatg cctttaaaaa ccgcctgcag gacattgccc agtacgccca
     6901 ggctgatatc aggcgtaacc aggaagctgt cacgctgaaa ctggtcagca aggactggaa
     6961 ttttgacatc gtcccctgct ttattaccag cgaagatgcc tttggacgga cttactacct
     7021 gatccccgat ggcaacggcc actggaagtt taccgacccc cgtaaagaca gggacagagt
     7081 cactactatc aatgtgcaga ataacggcaa tgtgctcaat gtcatcagag cagtcaaata
     7141 ctggcagcgg cgaccgacga tgccatccat gagttcctac ctgctggaaa cgctgattct
     7201 ggactattac gctggcagga catcctgctc atcgtttgtt gacatggagc tggaagcgct
     7261 tttccgccac cttggtcaat ccgtgcggta ctctgtcaac gatcccaaag gcatacaggg
     7321 tgatatcaac tcactttcag ccgaggcacg taaggcgata tcggacagat gttatctcga
     7381 tgcgcaaaag gtatcggagg cgagatggtt tgaaaataat aaagagtatg aaaaatccat
     7441 caataaatgg cgcgatgtgt tcgggccatt ctttcctgtt tacgggtgaa tcatgaatgc
     7501 catttacaca aaccagtcga ccagagaaaa catggattta ctgtatgcgc aggcccgtgt
     7561 ctacgacagg gtaaaaaact ggaacagact gaattttctc ttcagcatca tcgtaccgct
     7621 cctgctttct ttcgttaccg tatacaaccg gagccgggaa tttgtcgatt cagagttgtt
     7681 gtcttcgctg ttggggttat atggactcct ggtgcttacc tttaacattg ttatcagtgg
     7741 acacatctcg gctctgcgca ggaaggcagc ctcaatccag gaaatgtacg actgccgggt
     7801 acttggcatc cggcgtaatg aactgaaggt ggaggagatc ccacgggatg acatcatccg
     7861 ggaagctgca tattttagag acagcccgga aaaagcacga aagcgatttg gcgaagaggg
     7921 gtggtacgtc agtaaggtgt atgacgcgcc gcaggccgta atggcgctcc tttgccacgg
     7981 aaaaaatctc ggctgggaca aatcgctcag ggaggttctg cacgtatttt atctttccgc
     8041 ttttattgtc tcgcctgtcg cgatgcttgt ttacggcatc gtcatgaaat ccgggctgaa
     8101 tgagatcctc ttctatgtgg tgttcacact gccggtcatc cgctatttct tgcttcagtt
     8161 tctggataat cgcagcagta tgaaaagaag cgagaagctg aaaaagtacg ttgagaagga
     8221 attatccggc ataagggtgt ccggaagggc agaagaagag caactggctt atacgctccg
     8281 gaatatccaa gatgagatgt ttacctaccg ggcctcatgt ccgcccgtac ccaacggtat
     8341 tcagctgata atgaagccca aaaatgaacg aatttatgtt gattactttg aaacaaatct
     8401 gaaggagctg caccttcaga agtaatccct ccttagctcc ggtgtaggat ttacgtatgc
     8461 cggagcacat ccgataaaca attcttggtt gcctgagtgt cagcgtaaac ccatagtcac
     8521 cttcgcctca gacggttagt aaca
//
LOCUS       DAPOZO010000154         8497 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPOZO010000154 DAPOZO010000000
VERSION     DAPOZO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.154"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..669
                     /locus_tag="ROI21_004422"
     CDS             <1..669
                     /locus_tag="ROI21_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HDX5113260.1"
                     /translation="ELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQG
                     RLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLK
                     KLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWRVENKLHWRLDVV
                     MNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAG
                     SGLS"
     gene            867..3104
                     /gene="nrfB"
                     /locus_tag="ROI21_004423"
                     /pseudo
     CDS             867..3104
                     /gene="nrfB"
                     /locus_tag="ROI21_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            3091..6063
                     /gene="nfrA"
                     /locus_tag="ROI21_004424"
     CDS             3091..6063
                     /gene="nfrA"
                     /locus_tag="ROI21_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDX5113261.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQVDTLYNEARQQNTLS
                     AAERRQWFDVLLAGHLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNHQYVVGATLPVLLKKGQYDA
                     AQKLLATLPANEMLEERYTVSVATHNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIVFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYVRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIRTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6064..6954
                     /locus_tag="ROI21_004425"
     CDS             6064..6954
                     /locus_tag="ROI21_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDX5113262.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKTTGVNVWVQDGSGVNKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7137..7898
                     /gene="envY"
                     /locus_tag="ROI21_004426"
     CDS             7137..7898
                     /gene="envY"
                     /locus_tag="ROI21_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDX5113263.1"
                     /translation="MQLSSSEPCVVIQTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7944..8020)
                     /locus_tag="ROI21_004427"
     tRNA            complement(7944..8020)
                     /locus_tag="ROI21_004427"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7984..7986),aa:Arg,seq:tct)
     gene            8263..>8497
                     /locus_tag="ROI21_004428"
     CDS             8263..>8497
                     /locus_tag="ROI21_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDX5113264.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTV"
BASE COUNT         2172 a         2153 c         2179 g         1993 t
ORIGIN      
        1 gaacttctta acatgctgga tattaaagga aaaatcatca caactgatgc gatgggttgc
       61 cagaaagata ttgcagagaa gatacaaaaa cagggcggtg attatttatt cgctgtaaaa
      121 ggaaaccagg ggcggctaaa taaagccttc gaggaaaaat ttccgctgaa agaattaaat
      181 aatccagagc atgacagtta cgccattagt gaaaagagtc acggcagaga agaaatccgt
      241 cttcatattg tttgcgatgt ccctgatgaa cttattgatt tcacgtttga atggaaagga
      301 ctgaagaaat tatgcgtggc agtctctttt cggtcaataa tagcagaaca aaagaaagag
      361 ccagaaatga cggtcagata ttatatcagt tctgctgatt taaccgcaga gaagttcgcc
      421 acagcaatcc gaaaccactg gcgcgtggag aataagctgc actggcgtct ggacgtggta
      481 atgaatgaag acgactgcaa aataagaaga ggaaacgccg cagaattatt ttcagggata
      541 cggcacatcg ctattaatat tttaacgaat gataaggtat tcaaggcagg gttaagacgt
      601 aagatgcgaa aagcagccat ggacagaaac tatctcgcgt cagtccttgc ggggagcggg
      661 ctttcgtaat cttgccctgc gttaaccgcc cctccttgac accacgcttt tgtctacatt
      721 ctacacaaac ggcaactttt ataaccagtg taaaaataac gtgccgcaat agtcatacct
      781 attaatggta aaaagctgtc acaattcata aaaaacctta atatacgcca ccctaaacat
      841 aaccagcgtt aatgtaaggt ttttgtgtgg actggcttct tgatgttttt gctacctggc
      901 tctacggctt aaaagtaatc gcgataacgt tagcggtcat catgttcatc agcgggctgg
      961 acgatttttt tattgatgtc gtctactggg tacgccgcat taaacgcaag ttgagtgttt
     1021 atcgccgcta cccgcgaatg agttaccgcg aactgtataa accagatgaa aaaccgttag
     1081 cgattatggt tccggcgtgg aatgaaacgg gcgtcatcgg caatatggcc gagctggcgg
     1141 cgaccacgct cgactatgaa aactatcata tctttgttgg cacctacccc aacgaccccg
     1201 atacacagcg tgatgttgac gaagtgtgcg ctcgcttccc gaacgtgcat aaggtagtct
     1261 gcgcgcgtcc tggccccacc agcaaagccg actgcctgaa caacgtgctg gacgccatca
     1321 cccagtttga gcgtagcgcc aatttcgctt ttgctggttt tattctgcat gacgccgaag
     1381 acgtaatttc accgatggaa ttgcgtctgt tcaactatct ggtcgagcgt aaagatctga
     1441 ttcagatccc ggtgtatccg ttcgaacgcg aatggacgca cttcaccagc atgacttaca
     1501 ttgatgagtt ttcagaactg catggcaaag atgttccggt gcgtgaagcc ctcgccggac
     1561 aagtgcccag cgcaggcgtc ggtacctgtt tcagccgccg cgccgtgacc gcactgttag
     1621 ctgacggtga cggtattgct ttcgacgtgc agagtcttac tgaagattac gacattggtt
     1681 tccgcctgaa agaaaaaggt atgacggaaa tttttgtccg ttttccggtg gtggacgaag
     1741 ccaaagaacg cgagcagcgt aaatttttac agcacgcgcg gacgtcaaac atgatctgcg
     1801 tgcgcgaata tttccctgat accttttcga ctgcggttcg acaaaaatct cgctggatca
     1861 tcggcattgt tttccagggc tttaaaaccc acaaatggac ctccagtctg acgctgaact
     1921 actttctctg gcgcgaccgc aaaggggcaa tcagtaactt tgtcagcttc ctcgcgatgc
     1981 tggtgatgat ctagcttttg ctgttgctgg cgtatgaaag tttgtggccc gatgcctggc
     2041 atttcctttc tattttcagc ggcagcacat ggttaatgac tctgctgtgg ctaaactttg
     2101 gcttgatgat taaccgcatc gtgcagcggg tgattttcgt tactggctac tacggcctga
     2161 cgcaggggct gctatctgtc ctgcgtcttt tctggggcaa cctgattaac ttcatggcca
     2221 actggcgcgc gttaaaacag gtacttcaac acggcgatcc acgtcgtgtc gcgtgggata
     2281 aaacaacgca tgacttcccc agcgtgactg gcgatacccg ctcgttgcgt ccgttaggtc
     2341 aaatcctgct ggaaaatcag gtcatcactg aagaacaact cgatacagca ctgcgtaatc
     2401 gcgtcgaagg tctacgcctg ggcggttcaa tgctgatgca ggggctgatt agcgccgaac
     2461 agctggcaca ggcgctggca gagcaaaacg gcgtggcgtg ggagtcgatc gacgcctggc
     2521 agatcccttc atcactgatt gccgaaatgc cggcttccgt ggcgctgcat tatgcagtac
     2581 tgccgctgcg tctggaaaat gatgagttaa ttgtcggcag tgaagatggt attgacccgg
     2641 tttcgctggc agccctgacg cgtaaagtcg gacgcaaagt gcgttacgtc attgttctgc
     2701 ggggacaaat tgtcacgggg ttacgtcact ggtatgcgcg ccgacgcggt cacgatccgc
     2761 gggcaatgtt gtacaatgcg gttcagcatc agtggctcac ggaacagcag gccggtgaaa
     2821 tctggcggca atatgtgccg catcagttcc tgttcgccga aatactgacc acgctcggtc
     2881 atattaatcg ttcagcaatt aacgtgttgt tattgcgcca tgaacgcagt tctctgccgc
     2941 tcggcaagtt tttggtcacc gaaggcgtta tcagccagga aacgttggat cgcgtcctga
     3001 caattcaacg cgaattacaa gtttcgatgc aatcactatt actcaaagca ggtttaaaca
     3061 cagaacaggt tgcgcaactg gagtccgaaa atgaaggaga ataaccttaa tcgcgtcatc
     3121 ggatggtctg gtttactgct gacgtcttta ttgagtacca gcgcactcgc agacaatatc
     3181 ggcaccagcg cagaagagct ggggctgagc gattatcgcc attttgttat ttatccccgt
     3241 ctcgataagg cgctgaaggc acagaaaaat aacgacgaag caaccgccat ccgcgaattt
     3301 gaatatatac accaacaggt gccggataat attccgctaa ctttatacct tgcggaagcc
     3361 tatcgccatt ttggtcatga tgaccgggcg cggctgttgc ttgaggatca actgaaacgt
     3421 cacccaggag atgcccgact tgagcgcagt ctggcggcta ttccagttga agtgaaaagc
     3481 gttacgactg ttgaagaact gcttgcccag caaaaagcgt gcgacgctgc gccgaccctg
     3541 cgttgtcgca gtgaagtcgg gcagaatgcc ctgcggctgg cacaattacc tgtcgccaga
     3601 gcgcaactga acgatgcgac gtttgctgca tcgccggaag gaaaaacgct gcgaaccgat
     3661 ctgctgcaac gcgcaatcta cctgaaacaa tggtcccagg tagatacgct atacaatgaa
     3721 gcacgccagc agaacacatt aagcgcggca gaacgccgcc agtggtttga tgtgcttctt
     3781 gccgggcatc tggacgatcg gatcctggcg ctgcaatcac aggggatctt caccgatcct
     3841 cagtcatata ttacttacgc gaccgcgctg gcttatcgtg gcgaaaaagc acgcctccag
     3901 cattatctca ttgaaaatac gccgctattt accacggacg cacaagagaa aagttggctc
     3961 tatctgttat ctaaatacag cgccaacccc gttcaggcgt tggcgaatta tacggtacag
     4021 tttgccgaca accaccagta tgttgttggc gcgacgctac cggtgctgtt aaaaaaaggt
     4081 cagtacgacg cagcgcaaaa actgctcgcc accctccccg ccaatgaaat gcttgaggag
     4141 cgttataccg ttagcgtagc gacccataac aaggctgaag ctctgcgtct ggcacgattg
     4201 ctgtatcagc aagaaccggc aaatcttacc cgcctggatc aactgacctg gcaactgatg
     4261 cagaacgagc agtcacgcga agctgccgat ttattgctgc aacgctatcc tttccagggc
     4321 gatgcgcgtg tcagccagac tttaatggcg cgactggcgt ctctgctgga aagtcatcct
     4381 tacctggcaa cgccggcgaa ggtggcgatt ttatcgaaac ccttaccgct ggcggagcaa
     4441 cgtcagtggc aaagtcagtt gccgggtatt gcagataatt gcccggcaat agttcgcttg
     4501 ctgggcgata tgtcgccttc ctacgatgcc gccgcctgga accgtctggc aaagtgttat
     4561 cgggacacgc tacccggtgt ggcgttgtat gcatggcttc aggccgaaca acgacaaccg
     4621 aacgcctggc aacatcgtgc ggtagcctat caggcgtatc aggttgagga ctacgccacc
     4681 gcactggcgg cctggcagaa aatcagtctt cacgacatga gcaatgagga tctgcttgct
     4741 gctgccaata ccgcccaggc ggcaggaaat ggtgcggctc gcgatcgctg gcttcagcag
     4801 gcagaacaac gtgggctggg aaacaatgcc ctctactggt ggctgcatgc gcaacgttac
     4861 attcctggtc agccggaact cgcactgaac gatctcacgc gctcaatcaa tattgcgcct
     4921 tctgccaacg cttacgttgc gcgggcgaca atttatcgcc aacgtcataa tgtcccggcg
     4981 gcagtgagtg atttgcgcgc cgcgctggaa ctggagccga ataatagcaa cacccaggca
     5041 gcgcttggtt acgccttgtg ggatagcggt gatatcgcac agtcgcggga aatgctcgaa
     5101 caggcgcata aagggctacc ggacgatccg gcacttatcc gacaactggc ctacgtgaac
     5161 cagcgtctgg atgacatgcc tgcgacgcag cactacgccc ggctggtgat tgatgacatt
     5221 gataatcagg cgctgataac cccactgacc ccagagcaaa atcagcaacg cttcaatttc
     5281 cgccgtctgc atgaggaggt cggtcgccgc tggacgttca gtttcgattc ttccatcggc
     5341 ttgcgttccg gcgcgatgag taccgctaac aataatgtcg gcggcgcagc gccagggaaa
     5401 agctatcgta gctacgggca actggaagcc gagtaccgca tcggacgcaa tatgctgctg
     5461 gaaggcgacc tgctctcagt ttatagccgc gtctttgccg ataccggaga aaacggggtg
     5521 atgatgccgg tgaaaaatcc gatgtccggc accggtctgc gctggaagcc gctgcgcgat
     5581 cagatcgttt tcctcgccgt cgaacagcag ttgccgctga acgggcaaaa tggcgcatcc
     5641 gataccatgc tgcgcgccag cgcctcattc tttaatggcg gcaaatacag cgacgaatgg
     5701 cacccgaacg gttcaggctg gtttgcccaa aacctgtacc tcgatgcggc gcaatatgtc
     5761 cgccaggata ttcaggcgtg gacggcagat tatcgcgtaa gctggcatca gaaggtggcg
     5821 aacggacaga ctattgagcc ttacgctcac gttcaggaca acggttatcg tgataaaggc
     5881 actcagggcg cgcagcttgg cggtgtcggg gtccgctgga atatcaggac cggcgagacg
     5941 cactacgacg cctggccgca caaagtcagt ctcggcgtcg agtatcaaca tacctttaag
     6001 gcgattaatc aacgtaacgg agagcgcaac aacgcgtttc tcaccattgg agtgcactgg
     6061 taaatgcgta agttcatttt cgtattgctg acactgcttt tggtcagccc tttttccttt
     6121 gcgatgaaag gtattatctg gcaaccacaa aaccgtgata gtcaggttac cgatacccag
     6181 tggcaggggc tgatgagtca gttacgtttg caaggcttcg ataccctggt tttgcaatgg
     6241 actcgttacg gcgatgcatt cacccagcca gaacagcgcg cgttattgtt taagcgtgca
     6301 gcagctgcgc aacaggctgg cctgaagctg attgtcgggc tgaatgccga tccggaattt
     6361 tttatgcacc agaaacagtc atccgcggcg ctggaaagct atcttaatcg cctgctggct
     6421 gccgatctcc aacaagccag attatggagc gccgcgcctg gcgtaacgcc ggatggctgg
     6481 tacatcagcg cggaaattga cgacctgaac tggcgcagcg aagccgcccg tcagcctttg
     6541 ctgacatggt taaacaacgc gcagcggttg attagcgatg tttcagcaaa accggtttat
     6601 atcagtagtt ttttcgccgg aaacatgtcg cccgatggct atcgccaact gctggaacac
     6661 gttaaaacaa ccggcgttaa tgtctgggta caggatggca gcggcgtgaa taaacttaca
     6721 gctgaacagc gtgaacgtta tctacaggcc agcgccgatt gccaaagttc cgcccctgcc
     6781 agcggcattg tttatgaact gtttgtcgcc ggcaaaggca aaacctttac agcgaaaccg
     6841 aaaccggatg cagagatcgc ctcgctgtta gcgaaacgtt cctcttgcgg taaagacacg
     6901 ctctatttct ctctgcgcta tttgcccgtc gcgcacggca ttctcgagta ttaaatcccc
     6961 tccaggtaag tcgggtacga cctgacttac cactttcgct ctcgtcagaa ttccatcaag
     7021 attaattgct aaaaagcgtg ctaaagaaag aagatcgtta tcggcgaacg ggccgatcac
     7081 tcatgcagga agcatagatc ctttgagcgg agtttctgat atctgaggag tgcgaaatgc
     7141 aattgagcag cagtgaacct tgcgtggtga tccagaccga aaaagaggta gaggtaagcg
     7201 tcaataacca tgctacgttt acccttccga aaaactacct ggccgccttc gcgtgcaaca
     7261 ataacgtcat tgaactctca acgttaaatc acgtattaat cacccacatc aaccgcaaca
     7321 tcatcaacga ttatctgttg tttttaaata agaacttaac ctgcgtaaag ccctggtcgc
     7381 ggctggctac cccggttatc gcttgtcata gccgtacacc ggaagtgttc cggctagccg
     7441 ccaaccacag caagcagcaa cccagcaaac cctgcgaagc ggagttgacg cgcgcattgc
     7501 tttttaccgt attgtctaac tttcttgagc aatcgcggtt tattgcccta ctgatgtata
     7561 tcttacgcag cagcgtccgc gacagcgtct gccgcattat tcaaagcgat attcagcatt
     7621 actggaatct gcgaattgtc gccagttcgc tatgtttaag ccccagcctg ctcaaaaaga
     7681 aattaaaaaa cgaaaatacc agctatagcc agattgtcac agagtgtcgt atgcgttacg
     7741 ccgtacagat gttattgatg gataacaaaa atattactca ggtggcgcaa ttatgtggct
     7801 atagcagcac gtcgtacttt atctctgttt ttaaggcatt ttacggcctg acaccgttga
     7861 attatctcgc caaacagcga caaaaagtga tgtggtgaag ggcaaagcgg aaacggataa
     7921 gacggggata aatgaagaag aaatggcgcg ccctgcagga ttcgaacctg cggcccacga
     7981 cttagaaggt cgttgctcta tccaactgag ctaagggcgc gttgataccg caatgcggtg
     8041 taatcgcgtg aattatacgg tcaacccttg ctgagtcaat ggcttttgat ctggttgctg
     8101 aacaagtgaa cgaccgcgtc tgattttctg atttatttcg ctatagcggc aaacaaacgt
     8161 acaccgctgc gcgtctgaat caagaaaacc cgtattttca tgtatcaaag tacaatttcc
     8221 cgccctaacg gaaaattgtc cgatccaatg agactggtaa ctatgaaacc aacgtcggtg
     8281 atcattatgg atactcatcc tatcatcaga atgtctattg aagttctgtt gcaaaaaaac
     8341 agtgaattgc aaattgtcct gaaaacggat gattatcgca taaccatcga ttatctccga
     8401 acccgtcctg ttgatttaat cattatggat atagacttgc ccggaacaga cggttttacc
     8461 ttcctgaaaa ggatcaaaca aatccagagc acagtga
//
LOCUS       DAPOZO010000155         8403 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPOZO010000155 DAPOZO010000000
VERSION     DAPOZO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.155"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(24..236)
                     /gene="rpmE"
                     /locus_tag="ROI21_004429"
     CDS             complement(24..236)
                     /gene="rpmE"
                     /locus_tag="ROI21_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDX5113265.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            439..2637
                     /gene="priA"
                     /locus_tag="ROI21_004430"
     CDS             439..2637
                     /gene="priA"
                     /locus_tag="ROI21_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDX5113266.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2793..3818
                     /gene="cytR"
                     /locus_tag="ROI21_004431"
     CDS             2793..3818
                     /gene="cytR"
                     /locus_tag="ROI21_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDX5113267.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            3910..4869
                     /gene="ftsN"
                     /locus_tag="ROI21_004432"
     CDS             3910..4869
                     /gene="ftsN"
                     /locus_tag="ROI21_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDX5113268.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            4962..5492
                     /gene="hslV"
                     /locus_tag="ROI21_004433"
     CDS             4962..5492
                     /gene="hslV"
                     /locus_tag="ROI21_004433"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDX5113269.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5502..6833
                     /gene="hslU"
                     /locus_tag="ROI21_004434"
     CDS             5502..6833
                     /gene="hslU"
                     /locus_tag="ROI21_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDX5113270.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            6900..7826
                     /gene="menA"
                     /locus_tag="ROI21_004435"
     CDS             6900..7826
                     /gene="menA"
                     /locus_tag="ROI21_004435"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDX5113271.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            7919..>8403
                     /gene="rraA"
                     /locus_tag="ROI21_004436"
     CDS             7919..>8403
                     /gene="rraA"
                     /locus_tag="ROI21_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDX5113272.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         1994 a         2202 c         2327 g         1880 t
ORIGIN      
        1 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
       61 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      121 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      181 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      241 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      301 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      361 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      421 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttccccgt
      481 acctttgact atctgctgcc agaaggcatg acggtcaaag ccgggtgtcg cgtgcgcgtg
      541 ccgtttggca aacagcagga gcgcatcggg gttgtggtgt cagttagcga tgtcagcgag
      601 ctgccgctca acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtgtttact
      661 cactccgtct ggcgattact gctatgggcg gcggattact atcatcatcc gattggcgat
      721 gtgctgtttc atgccttgcc gattttacta cgccaggggc ggcctgcggc gaacgcgccg
      781 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgt
      841 tcgcccaagc aacaacaggc gctggcagcg ttacggcaag gcaaaatctg gcgtgatcag
      901 gtcgcggaac ttgaatttaa tgatgccgcg ttgcaggcgc tgcgcaaaaa aggtctgtgt
      961 gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1021 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1081 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1141 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1201 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac gttttaatgc ccccgtggaa
     1261 gttctgcatt ccggcctgaa cgacagcgag cgtctttcgg cgtggttgaa agcgaaaaat
     1321 ggtgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1381 ggcgtgattg tcattgatga agagcacgac agttcctaca agcagcagga aggctggcgc
     1441 tatcatgccc gcgacctggc ggtttatcgt gcgcacagtg agcaaatccc gattattctt
     1501 ggctccgcaa cacccgcact ggaaacgtta tgcaacgtcc agcagaaaaa ataccgcctg
     1561 ctgcgcctga cgcgtcgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1621 aaaggtcaga aggtgcaggc ggggctggct ccggcgttaa tcacccgtat gcgccagcat
     1681 ttacaggccg acaaccaggt tatcctcttt cttaaccgcc gtggctttgc gcctgccttg
     1741 ctgtgccacg actgcggctg gattgccgaa tgtccacgtt gcgatcacta ctacacgctg
     1801 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gccaacgccc ggtgccgcgc
     1861 cagtgccctt cctgcggttc cacacacctg gtacccgtgg ggctgggcac cgaacagctt
     1921 gaacagacgc tcgcgccgct gttccccggc gtgcccattt ctcgtatcga ccgcgatacc
     1981 accagccgca aaggggcgct ggaacagcaa ctggcggaag tgcatcgcgg cggcgcgagg
     2041 atcttgattg gcacgcaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggta
     2101 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2161 gcccagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2221 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2281 gacgcctttg ccgaacaggc gctggctgag cgacggatga tgcagctgcc gccgtggacc
     2341 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2401 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaactgtg ggttctcggt
     2461 ccggttccgg cgctggcacc taaacgtggc ggtcgctggc gctggcaaat tttgttgcag
     2521 cacccttccc gcgtgcgctt acagcacatc atcaatggta cgctggcact tattaataca
     2581 ataccggatt cccgtaaggt gaaatgggtg ctggatgtcg atcccatcga gggctaaacc
     2641 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     2701 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     2761 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     2821 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     2881 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     2941 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3001 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3061 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3121 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgttgc
     3181 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3241 tgatggcgaa cgaatttgca ccggaactgg agctgcctac cgttcatatc gacaatctga
     3301 ccgccgcatt tgatgcagta aattatttat atgagcaagg gcataaacgg attggctgta
     3361 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggctac gttcaggcgc
     3421 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3481 aagccggaag caaagcaatg caacagctgc ttgatcttcc acaaccgcct actgctgtct
     3541 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3601 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3661 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     3721 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     3781 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     3841 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     3901 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     3961 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4021 gctattgctg ccgccgttct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4081 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggaaacgg actaccacca
     4141 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc aggtgtgcgt
     4201 gcgcctacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact aacaccagag
     4261 caacgccagc tccttgagca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4321 gaagttccgt ggaatgagca gacgccggaa cagcgtcagc aaacgctaca gcgccagcgt
     4381 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4441 agctggcagc agcagacgcg tacgtcgcaa gccgcaccgg tacaggcgca accgcgccag
     4501 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaactcc tgcacacacg
     4561 actgcgcagt cgaagccgca gcaagctgcg ccagttgctc gtgccgctga cgcgccaaaa
     4621 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4681 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggctttga ctcgaaaatc
     4741 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     4801 gacagcaccc tcaatcgctt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     4861 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     4921 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     4981 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5041 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5101 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5161 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc gaaagactgg cgtaccgatc
     5221 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5281 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5341 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5401 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5461 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5521 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5581 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5641 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     5701 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     5761 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     5821 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     5881 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     5941 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caagaaactg cgtgaaggcc
     6001 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6061 tggctcctcc gggaatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6121 gcggccagaa gcaaaaagca cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6181 aagaagaagc ggcgaaactg gtgaatccgg aagagctgaa gcaagacgct atcgacgctg
     6241 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6301 cttccggtcc ggatgtttct cgtgaaggtg ttcagcgtga cctgctgccg ctggtagaag
     6361 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6421 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcgtctgc
     6481 caatccgcgt tgagctgcag gcactgacca ccagcgactt cgaacgtatt ctgaccgagc
     6541 cgaatgcctc tattaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6601 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6661 ccgaaaacat cggtgctcgt cgtttacata ccgttctgga gcgtttaatg gaagagattt
     6721 cctacgacgc cagcgattta agcggtcaaa ccatcactat tgacgcagat tatgtgagca
     6781 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     6841 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     6901 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     6961 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7021 tcgatccgct ggtcgccctg ctggcactga ttaccgccgg gctattacag atcctttcta
     7081 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgtatcggac
     7141 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7201 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggcg tgccatacgc
     7261 tggccgattt tgtcggcttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7321 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7381 tctttggctg gttgagcgtc atgggcagct ggtatttaca ggctcataca ttgattccgg
     7441 cgctgatcct tccagcgacc gcatgcggcc tgctggcaac ggcagtactg aacattaata
     7501 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7561 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtgtgcctgg
     7621 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcgc
     7681 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     7741 cgatgctgga acgcactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     7801 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     7861 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     7921 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     7981 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8041 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8101 ggtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8161 ggcagtacaa aatgaatggg aaggtctggt catttacggc gcggtgcgtc aggtagatga
     8221 cctggaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgctgctgg
     8281 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtcacct tcttctccgg
     8341 cgaccatctt tatgccgaca ataccgggat tattctttct gaagatccgt tggatattga
     8401 atg
//
LOCUS       DAPOZO010000156         8213 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPOZO010000156 DAPOZO010000000
VERSION     DAPOZO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.157"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            124..543
                     /gene="rbsD"
                     /locus_tag="ROI21_004437"
     CDS             124..543
                     /gene="rbsD"
                     /locus_tag="ROI21_004437"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDX5113273.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            551..2056
                     /gene="rbsA"
                     /locus_tag="ROI21_004438"
     CDS             551..2056
                     /gene="rbsA"
                     /locus_tag="ROI21_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDX5113274.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            2061..3026
                     /gene="rbsC"
                     /locus_tag="ROI21_004439"
     CDS             2061..3026
                     /gene="rbsC"
                     /locus_tag="ROI21_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDX5113275.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            3051..3938
                     /gene="rbsB"
                     /locus_tag="ROI21_004440"
     CDS             3051..3938
                     /gene="rbsB"
                     /locus_tag="ROI21_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDX5113276.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVK"
     gene            4067..4996
                     /gene="rbsK"
                     /locus_tag="ROI21_004441"
     CDS             4067..4996
                     /gene="rbsK"
                     /locus_tag="ROI21_004441"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDX5113277.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVINGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIH
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            5000..5992
                     /gene="rbsR"
                     /locus_tag="ROI21_004442"
     CDS             5000..5992
                     /gene="rbsR"
                     /locus_tag="ROI21_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDX5113278.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5958..7385)
                     /gene="mdtD"
                     /locus_tag="ROI21_004443"
     CDS             complement(5958..7385)
                     /gene="mdtD"
                     /locus_tag="ROI21_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDX5113279.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(7408..8100)
                     /locus_tag="ROI21_004444"
     CDS             complement(7408..8100)
                     /locus_tag="ROI21_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDX5113280.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2089 a         1985 c         2162 g         1977 t
ORIGIN      
        1 atggttaatc accatgtaaa acgtttcgag gttgatcaca tttccgtaac ttcacgatgg
       61 ttttcccaac tcagtcagga ttaaactgcg ggtcagcgaa acgtttcgct gatggagaaa
      121 aaaatgaaaa aaggcaccgt tcttaattct gatatttcat cggtgatctc ccgactggga
      181 cataccgata cgctggtggt gtgtgatgca ggtttaccca tccccaaaag tacaacgcgt
      241 atcgatatgg cattaaccca gggtgtacct tcttttatgc aggtgctggg cgtcgtcaca
      301 aatgaaatgc aggtcgaggc agccattatc gcggaagaga tcaaacaaca taatccgcaa
      361 ctccacgaaa cgttgctcac tcaccttgag cagctgcaaa aacaccaggg aaataccatt
      421 gaaattcgtt acaccacgca tgaacaattc aaacaacaaa ccgcagaaag tcaggcggta
      481 attcgcagcg gagaatgttc tccgtatgcg aatatcattc tctgtgctgg cgtgacgttc
      541 tgaggccgtc atggaagcat tacttcagct taaaggcatc gataaagcct tcccgggcgt
      601 aaaagccctc tcgggtgcag cgttaaatgt ctatcctggc cgcgtgatgg cgctggtggg
      661 cgaaaacggt gcgggtaaat ccaccatgat gaaagtgctt acgggtatct atactcgcga
      721 tgccggtacg cttttatggc tggggaaaga aacgacgttt accgggccga aatcttccca
      781 ggaagccggg attgggatta tccatcagga actgaacctg atcccgcagt tgaccattgc
      841 cgaaaacatt ttcctcggtc gtgagtttgt taatcgcttt ggcaaaattg actggaaaac
      901 catgtatgcc gaagcggata aattgctggc taaacttaac ctgcgcttta aaagcgacaa
      961 gttagtgggc gatctttcca tcggtgacca gcaaatggtt gaaatagcca aagtgctgag
     1021 ctttgagtcg aaagtcatca ttatggatga accgaccgat gcgctgaccg ataccgaaac
     1081 cgaatccttg ttccgcgtca tccgcgagct gaaatcgcaa ggccgcggta ttgtctatat
     1141 ttcccaccgc atgaaagaaa tcttcgagat ttgcgatgac gttaccgttt ttcgtgatgg
     1201 gcaatttatt gctgagcgtg aagtagcatc actgaccgaa gattcgctga ttgagatgat
     1261 ggtgggccgc aagctggaag atcaatatcc gcacctggac aaagcgccag gagatattcg
     1321 cctgaaagtc gataatctct gcggacctgg cgttaacgat gtctctttta ctttacgcaa
     1381 aggcgaaatt cttggcgtct ctggtttgat gggcgcaggt cgtaccgaac tgatgaaagt
     1441 gctctacggc gcactgccgc gcaccagcgg ttacgtcacc ctggatgggc atgaagttgt
     1501 tacccgttca ccgcaggatg gcctggcaaa cggcattgtg tatatctccg aagaccgtaa
     1561 acgtgacggt ttagtgctgg gcatgtcagt aaaagagaat atgtcgctga ccgcgctgcg
     1621 ctacttcagc cgcgctggcg gcagtttgaa gcatgccgat gaacagcagg cagtaagtga
     1681 tttcattcgt ctgtttaatg tgaaaacgcc gtcgatggaa caggcaattg gtctgctttc
     1741 cggtggcaat cagcaaaaag tggcgattgc ccgtggtctg atgacacgcc ccaaagtgtt
     1801 gatcctcgat gagcctaccc gtggcgtaga tgtcggcgcg aaaaaagaga tctatcaact
     1861 gattaaccag ttcaaagccg atggcttgag catcattctg gtgtcatcgg agatgccaga
     1921 agtattaggc atgagcgatc gcatcatcgt catgcatgaa gggcatctca gcggggaatt
     1981 tactcgtgag caggccaccc aggaagtgtt aatggctgcc gctgtgggca agcttaatcg
     2041 cgtgaatcag gagtaaaaaa atgacaaccc agactgtctc tggtcgccgt tatttcacga
     2101 aagcgtggct gatggagcag aaatcgctta tcgctctgct ggtgctgatc gcgattgtct
     2161 cgacgttaag cccgaacttt ttcaccatca ataacttatt caatattctc cagcaaacct
     2221 cggtgaacgc cattatggca gtcgggatga cgctggtgat cctgacgtcg ggcatcgact
     2281 tgtcggtagg ttctctattg gcgctgaccg gcgcagttgc tgcatctatc gttggcattg
     2341 aagtcaatgc gctggtggct gtcgctgccg ctctcgcgtt aggtgccgca attggtgcgg
     2401 taaccggggt gattgtagcg aaaggtcgcg tccaggcgtt tatcgctacg ctggttatga
     2461 tgcttttact gcgcggcgtg accatggttt ataccaacgg tagcccagtg aataccggct
     2521 ttactgagaa cgccgatctg tttggctggt ttgggatcgg tcgtccgctg ggcgtaccga
     2581 cgccagtctg gatcatgggg attgtgttcc tcgcggcctg gtacatgctg catcacacgc
     2641 gtttggggcg ttacatctac gcgctgggcg gcaacgaagc ggcaacgcgt ctttctggta
     2701 tcaacgtcaa taaaatcaaa atcatcgtct attctctttg tggtctgctg gcatcgctgg
     2761 ccgggatcat tgaagtggcg cgtctctcct ccgcacaacc cacggcgggg actggctatg
     2821 agctggatgc tattgctgcg gtggttctgg gcggtacgag tctggcgggc ggaaaaggtc
     2881 gcattgttgg gacgttgatc ggcgcattaa ttcttggctt ccttaataat ggattgaatt
     2941 tgttaggtgt ttcctcctat taccagatga tcgtcaaagc ggtggtgatt ttgctggcgg
     3001 tgctggtaga caacaaaaag cagtaataac gactacagga catcttgaat atgaacatga
     3061 aaaaactggc taccctggtt tccgctgttg cgctaagcgc caccgtcagt gcgaatgcga
     3121 tggcaaaaga caccatcgcg ctggtggtct ccacgcttaa caacccgttc tttgtatcgc
     3181 tgaaagatgg cgcgcagaaa gaggcggata aacttggcta taacctggtg gtgctggact
     3241 cccagaacaa cccggcgaaa gagctggcga acgtgcagga cttaaccgtt cgcggcacca
     3301 aaattctgct gattaacccg accgactccg acgcagtagg taatgctgtg aagatggcta
     3361 accaggcgaa catcccggtt atcactcttg accgccaggc aacgaaaggt gaagtggtga
     3421 gccacattgc atctgataac gtactgggcg gcaaaatcgc cggtgattac atcgcgaaga
     3481 aagcgggtga aggtgccaaa gttatcgagc tgcaaggcat tgccggtaca tccgcagccc
     3541 gtgaacgtgg cgaaggtttc cagcaggccg ttgctgctca caagtttaat gttcttgcca
     3601 gccagcctgc agattttgat cgcaccaaag gtttgaacgt aatgcagaac ctgttgaccg
     3661 ctcatccgga tgttcaggct gtattcgcgc agaatgatga aatggcgctt ggcgcgctgc
     3721 gcgcactgca aactgccggt aaatcggatg tgatggtcgt cggatttgac ggtacaccgg
     3781 atggcgaaaa agcggtgaat gatggcaaac tagcagcgac tatcgctcag ctacccgatc
     3841 agattggcgc gaaaggcgtc gaaaccgcag ataaagtgct gaaaggcgag aaagttcagg
     3901 ctaagtatcc ggttgatctg aaactggttg ttaagtagta gttttaatca ggttgtatga
     3961 cctgatggtg acataaatac gtcatcgaca gatgaacgtg taatataaag aaaagcaggg
     4021 cacgcgccac cctaacacgg tggcgcattt tatggacatc ccgaatatgc aaaacgcagg
     4081 cagcctcgtt gttcttggca gcattaatgc tgaccacatt cttaatcttc aatcttttcc
     4141 tactccaggc gaaaccgtaa ccggtaacca ctatcaggtt gcatttggcg gcaaaggcgc
     4201 taatcaggct gtggctgctg ggcgtagcgg tgcgaatatc gcgtttattg cctgtacggg
     4261 tgatgacagt attggtgaga gcgttcgcca gcagctcgcc actgataaca tcgatattac
     4321 tccggtcagc gtgatcaacg gcgaatcaac aggtgtggcg ctgatttttg ttaatggcga
     4381 aggtgagaat gtcatcggta ttcatgccgg cgctaatgct gccctttccc cggcgctggt
     4441 ggaagcgcaa cgtgagcgta ttgccaacgc gtcagcatta ttaatgcagc tggaatcacc
     4501 actcgaaagt gtgatggcag cggcgaaaat cgcccatcaa aataagacta tcgttgcgct
     4561 taacccggct ccggctcgcg aacttcctga cgaactgctg gcgctggtgg acattattac
     4621 gccaaacgaa acggaagcag aaaagctcac cggtattcgt gttgaaaatg atgaagatgc
     4681 agcgaaggcg gcgcaggtac tgcatgaaaa aggtatccat actgtactga ttactttagg
     4741 aagtcgtggt gtatgggcta gcgtgaatgg tgaaggtcag cgcgttcctg gattccgggt
     4801 gcaggctgtc gataccattg ctgccggaga tacctttaac ggcgcgttaa tcacggcgtt
     4861 gctggaagaa aaaccattgc cagaggcgat tcggtttgcc catgctgccg ctgcgattgc
     4921 cgtaacacgt aaaggcgcac aaccttccgt accgtggcgt gaagagatcg acgcattttt
     4981 agacaggcag aggtgatgct tggctacaat gaaagatgtt gcccgcctgg cgggcgtttc
     5041 tacctcaaca gtttctcacg ttatcaataa agatcgcttc gtcagtgaag cgattaccgc
     5101 caaagttgaa gcggcgatta aagaactcaa ttacgcgcca tcagccctgg cgcgtagcct
     5161 caaactcaat caaacacata ccattggcat gttgatcact gccagtacca atcctttcta
     5221 ttcagaactg gtgcgtggcg ttgaacgcag ctgcttcgaa cgcggttata gtctcgtcct
     5281 ttgcaatact gaaggcgatg aacagcggat gaatcgcaat ctggaaacgc tgatgcaaaa
     5341 acgcgttgat ggcttgctgt tactgtgcac cgaaacgcat caaccttcgc gtgaaatcat
     5401 gcaacgttat ccgacagtgc ctactgtgat gatggactgg gctccgttcg atggcgacag
     5461 cgatcttatt caggataact cgttgctggg cggagactta gcaacgcaat atctgatcga
     5521 taaaggtcat acccgtatcg cctgtattac cggtccgctg gataaaactc cggcgcgcct
     5581 gcggttggaa ggttatcggg cggcgatgaa acgtgcgggt ctcaacattc ctgatggcta
     5641 tgaagtcact ggtgattttg aatttaacgg cgggtttgac gccatgcgcc aactgttatc
     5701 acatccgctg cgtcctcagg ccgtctttac cggaaatgac gctatggctg ttggcgttta
     5761 ccaggcgctg tatcaggcag agttacaggt tccgcaggat atcgcggtga ttggctatga
     5821 cgatatcgaa ctggcaagct ttatgacgcc accattaacc actatccacc aaccgaaaga
     5881 tgaactgggg gagctggcga ttgatgtact catccatcgg ataacccagc cgacccttca
     5941 gcaacaacga ttacaactta ctccgattct gatggaacgc ggttcggctt agatttacgc
     6001 tgtcttttga tcaaattatt accatcggtt gttttcagaa gcatgaacat tgctgctgaa
     6061 gcaacagtaa taatgcccat cgtgataaac gtatagtgga attgttcgac tgtcgttgtg
     6121 ccttccatcc cttcataaac gcgaaggacg gccgcactta cagcaacgcc taaactgata
     6181 gacagttgct gcgtgaccgc cagaacactg ttaccgctgc tggcgttgtc atcggtcaga
     6241 tcggcaagtg tgatggtatt cattgcggta aattgcgtcg acatagccat ccctaatata
     6301 aacaacggca agatcagcat ccatatcgcc attgctggtg attgcaaaga gaactgagcg
     6361 atcattagcc caataatcac cgtgatcccc actaacgtat ggcgatagcc cagacgacgt
     6421 aagacttggg taaccatcga ttttgcaata atggaaccta acgctgtcgg tgccatcata
     6481 cagccggcaa taaacgcctg ataaccaaat cctacctgta acatcaatgg cataaggaac
     6541 ggtacacagc cggtccccag acgggttgca atattgccta cgataccgat cgagaaagtg
     6601 cgggttttaa ataaatctaa tgaaattaat gggtttggcg tgcgtcgtgc atggagaata
     6661 tagagaagca gtaacccgat gctggtgaca attaccgtca aggcaatcca actggcgaca
     6721 atcttttccc cgaatagctc tattccgctt gagaagagaa caaggctaag gccaaacagc
     6781 aaaaagccag tgatatcgaa tctgcgtcgt gcggtggtga aattgggcat atgtttgcgc
     6841 gcgtaaagaa ggcccgcaat acctatgggg atattgatta aaaatatcca gtgccaggtt
     6901 gcccaggtga ccagcacgcc gccaagaacg gggcctaaaa ttggccccac cagacccggc
     6961 atggcgacaa aattcaatac aggaagaagc tcattacgag gataagcgcg cagtaaggcc
     7021 agccgagcaa caggcatcat cattgcgccg cctatcccct gaataacccg gaagacaacc
     7081 agctgtggta gcgagttaga aagtgcgcag gccagagaac ccaacgtgaa cagactcacg
     7141 gcaagggtaa atatgcgacg cgtaccgaag cgatcggcta gccatccgct taccggaata
     7201 agcatcgcca ccgtcagcgt ataactgatg atggctgatt gcatcgcgag aggagaacga
     7261 ttaaggctat gagcgattgc gggtaaggcg gtattcagaa tagtggcatc aagtgcctgc
     7321 atgaagaagg ccatcgccgc gatccacggc aaacccgcca tactgcgctt ctttttatcg
     7381 ctcattcaat gtcctgttat cggggtgtca cttatcaggt gagcgtagca gcgcctgaca
     7441 agctttaaat gccgcgtcgc catcgctttg gataatcgcg tcaacaatcg cctggtgcag
     7501 atccagcttt atcactgtgt cgctggtaat tgacgtgaag taagtgtgat aaaccgaatg
     7561 gaatagcgag gcgaatgagg tcaaaaacgg attggcgctc atttcataga tatgctcatg
     7621 ccaagccata tcgacctcaa tccagcgttc acggcgaaag ttctctttta atgccaccat
     7681 ttcggccatt aacgtattga gatgcgcctt ctgttccgcg gtgccaaccg ttgcagccag
     7741 taggcaagct tgcggctcca gacagatacg cataaccaga aagtgatcga tgacctgatg
     7801 aaagttctct tctgtcatcc accaggtaag caattcctga tcaagaaaat tccagtttga
     7861 ttgtggcatg acccgggtac caattcgcgg tcgcggtaaa accatccctt ttgccgttaa
     7921 cgttttgacc gcttcgcgta ccgctgtacg actcactcca aattgctcgc ccagctcaat
     7981 ctcaccaggc aaaatggtgc cgggttcata ttcacctttt aagatgcgtt gcgccagctt
     8041 ctcagccaga acatacgaaa ggtttttctg tgcagctaac tgttgtgcgc ttaaaggcat
     8101 tactaatctt cctttttctt tttattcctc cttagtatgc caccaggaag tgtgattacg
     8161 gttgcaaaaa cggcaaattg cttgttttat ggcacattaa cggggctttt gct
//
LOCUS       DAPOZO010000157         8196 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPOZO010000157 DAPOZO010000000
VERSION     DAPOZO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.158"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            411..1235
                     /gene="gadX"
                     /locus_tag="ROI21_004445"
     CDS             411..1235
                     /gene="gadX"
                     /locus_tag="ROI21_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDX5113281.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHAHKKVPALLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDMQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDPAASIVAQGNFYGTDRSAEGIRL"
     gene            1604..2332
                     /gene="gadW"
                     /locus_tag="ROI21_004446"
     CDS             1604..2332
                     /gene="gadW"
                     /locus_tag="ROI21_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDX5113282.1"
                     /translation="MTHVCSVILVRRSFDIYHEQQKISLHNESILLLDKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQIPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2695..5808)
                     /locus_tag="ROI21_004447"
     CDS             complement(2695..5808)
                     /locus_tag="ROI21_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDX5113283.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5833..6990)
                     /gene="mdtE"
                     /locus_tag="ROI21_004448"
     CDS             complement(5833..6990)
                     /gene="mdtE"
                     /locus_tag="ROI21_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDX5113284.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGTLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTDSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKAAALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENVSTESKQ"
     gene            7050..7328
                     /locus_tag="ROI21_004449"
     CDS             7050..7328
                     /locus_tag="ROI21_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113285.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYIYQGNKVVFASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7329..7856)
                     /gene="gadE"
                     /locus_tag="ROI21_004450"
     CDS             complement(7329..7856)
                     /gene="gadE"
                     /locus_tag="ROI21_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDX5113286.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         2132 a         1897 c         1925 g         2242 t
ORIGIN      
        1 cacccgaaac tgcagggtat tgcccagcag aacagcttta aacacacctg ataacataac
       61 gttgtaaaaa ccgaatgccc aacctttaaa aaacagttgg gcattcggtt gcttattaat
      121 cgcaataata tattggctgt ctattcatcg tgttgataag atatatgtta tatgaatgtt
      181 tattcaatgt aacgggtgtt tttaatgtca ctacaaggga ttatttgctt actattaatt
      241 tcactgcgtt cttaagcacg ccttttgtat tataaatcca gtcatcctgc ccgtagatta
      301 tcatcctgtt ttcccgcttc gtttaaattt atttatcaat caatttgact taagagggcg
      361 gcgtgctaca ttaataaaca gtaatatgtt tatgtaatat taagtcaact atgcaaccac
      421 tacatggtaa ttgtctaatt gcgtacgcaa gacataaata tattctcacc atggttaatg
      481 gtgaatatcg ctattttaat ggcggtgatc tggtttttgc ggatgcaagc caaattcaag
      541 tggataagtg tgttgagaat ttcgtattag tttcaagaga cacgctttca ttatttctcc
      601 cgatgctcaa ggaggaggca ttaaaacttc atgcacataa aaaagttcct gcattactcg
      661 ttcatcactg taccagagat attcctgttt ttcaggaagt tgcgcaacta tcgcagaata
      721 agaatcttcg ctatgcagaa atgctacgta aaagggcatt aatctttgcg ttgttatctg
      781 tttttcttga ggatatgcag tttataccgt tactactgaa cgtgttgcaa ccgaatatgc
      841 gaacacgagt ttgtacggtc atcaataata atatcgccca tgagtggaca ttagcccgaa
      901 tcgccagcga gctgttgatg agtccaagcc tgttaaagaa aaaattgcgc gaagaaggta
      961 cgtcatattc acagttgctt actgagtgta ggatgcagcg tgctttacaa cttattgtta
     1021 tatatggtgt ttcaattaag cgagttgcag tatcctgtgg atatcacagc gtgtcgtatt
     1081 ttatttacgt ttttcgaaat tattatggga tgacgcccac agagtatcag gagcgatcgg
     1141 cgcagggatt gccgaaccgt gacccggcgg caagtattgt tgcacaaggg aatttttacg
     1201 gcactgaccg ttctgcggaa ggaataagat tatagagttt tactcagaca taaaaaaaac
     1261 ccggcatagg ggaccgggaa gaggatagtc tgccgtctcc agactaataa accgttataa
     1321 cactccctgt tggcacggga aactttgtgc tctcagtaag ttaaatataa cttttactgg
     1381 aaataagatc agccattttt ttataaacat aagctatacg ctgtgcgaaa atatattcgt
     1441 gctgcattta cttattatca attaactgtt atgcaaaact actttgtgga taaattttgg
     1501 tcctaccaaa tctggcagtt tttgcgctaa gaaacagtct ggcatcattt cattagtata
     1561 ctgaaattga aataatcgca gtaatgaaat ataagggata gtcatgactc atgtctgctc
     1621 ggtgatcctc gttcgtcgtt cattcgatat ttatcatgaa cagcaaaaaa tatcgttgca
     1681 caacgagagt atcctgttac tggataaaaa tttggcagac gattttgcgt tttgttcacc
     1741 ggatacgcga cgactggata tcgatgagct gacagtttgc cattacttac aaaatattcg
     1801 tcagatacca cgcaatttag ggttacatag caaagaccgt ctgttaatta accagtcacc
     1861 ccccatgccg ctggtgacgg cgatttttga tagtttcaat gaatcagggg taaattcacc
     1921 gatactgagc aaaatgctct atctttcctg tttatcgatg ttttctcata agaaagaact
     1981 gatcccctta cttttcaata gtatcagtac tgtttcagga aaagttgaac gccttattag
     2041 ctttgatatc gccaaacgtt ggtatctgcg cgatattgcg gaaagaatgt ataccagcga
     2101 gagtctcatc aaaaaaaagt tgcaggatga aaatacctgt ttcagtaaaa tattactcgc
     2161 ctccaggatg tcgatggcca gacgattact cgagttacgt caaattcctc tgcatactat
     2221 tgcggaaaaa tgtggctata gcagtacgtc gtactttata aacacatttc gacaatatta
     2281 tggtgtaacg ccacatcagt ttgcgcaaca ttcgccaggt accttttcct gacatatttt
     2341 gcatttgaat attggtcagg atctcacacc tgcttcatgt gaaactcttc cctgatgatt
     2401 tctgccgggc taccggctag ttctctttcg cagtttgcca ggcggtatag gtattttcag
     2461 gaggaaaaga ctcggcatgt ttgggattat taagctgaca attcatacga ttacgggaat
     2521 gtgggtcagt attgtgttat tcaaattgat gacgaatggc tggagtggtt tctactttca
     2581 gtgctgtgta ctaagcctgg tattcctgac ggttagctgg ttgttgagtg gtgaatggtt
     2641 ggcaggaaag agtaaggctg aaccctcacg ttcaacctta ctctcattta cacgttacgc
     2701 ttttttaaag cgggcaaaga gatgttccac tacaacgaaa aagaccggaa cgaagtaaat
     2761 tgccagcact gttgcggcaa acatcccgcc catcacgccg gtacctaccg cgttttgcgc
     2821 gccagaaccg gcaccatgac tgataaccag cggcagcacg ccgagaataa aggccagaga
     2881 ggtcatcagg attgggcgta aacgcatccg cgccgcttcg atgattgcct ctatcggcgt
     2941 tttcccttct ttctgcatca tctcaacggc aaattcgaca atcaggatgg cgttcttggc
     3001 ggaaagcccg atggtggtca gcaaaccaac ctggaagtag acgtcattac ttaagccgcg
     3061 tagatcggtg gccagtaatg cgccaacgac gcctaacggt acaaccaaca tcaccgagaa
     3121 cggaattgac cagctctcat aaagtgcggc gagggcgagg aacaccacga ccagtgaaat
     3181 ggcatacagc gcgggagcct gatttgagga taacgcttcc tgatacgaca gtccggtcca
     3241 tgagtagccg acgcctgccg gaagtttagc gaccaggtct gccataaatt tcatggcgtc
     3301 accggtactt ttcccggccg ccgcttcacc taaaatctcc attgacggga tgccgttgta
     3361 gcgttccagt cgcggtgaac cataggtcca ttcggtagac gaataggcag aaagcggtgc
     3421 catcgtgcca gaggcgttgc gtacatacca ttggttgatg ttatccggca acatacggaa
     3481 cggcgtgcct gcctggacat acactttttt cacccgcccc tggttgagga agtcgttcac
     3541 gtagctgctg ccgaaggcgg tggaaattgt ctggttgata tcagacagcg ccacacccat
     3601 tgcctcagct ttcgcggcgt tgacgttcac tttgaacatc ggcgtatctt ccagtccgtt
     3661 cggacgtacc ccggtgactt ggttcggtga ttgcgctgcc agtgataata gctcgtttcg
     3721 cgcctgggtc agtttttcgt gccccaggtt gccgttgtcc agcagttcca tatcaaaacc
     3781 tgacgcggta cccagttcag ccaccgcggg taagttgaac gggaagacga cggctttatt
     3841 gatactgctt aacgcaatca ttgcccgctg aatgatcgcg gtaaccgagt tttcctcgcc
     3901 gacacgttca gaccacggtt tgaggctgat aaacgccaga ccgttgtttt gcccctggcc
     3961 gctgaagcca aagccgccaa cggtaaacac cgactgaaca ttatctttct ctttagtcag
     4021 ataataatcc gtcacctgtt gcagcacttt cgtggtgtta accatggtgg caccggaagg
     4081 taactgcgcg gtggtcataa ataccccctg atcctcttct ggtaagaaag aggtcggcgt
     4141 gcgcaggaac agcaccgcca tcccggcgca aatcagcagg tagaccacca tgtagcgacc
     4201 ggtacaacgc aacagcgagc gggtactgtc ggtatagtgc tgagttgatt tttcaaacag
     4261 cgtgttgaag cgtgcgaaca gggcgttagg tttgtgaccg ccttccggcg cggctttcag
     4321 aatggtggcg cacagggcag gggtcaggct cattgccaca aatactgaaa gcagcatgga
     4381 ggagatcagc gtgatggaga actggcggta gatctccccg gttgcaccgc tcataaaggc
     4441 catcggcata aacactgcgg aaagaacaac ggcaatcccg accagcgcac gttggatctg
     4501 ccccatcgat ttatgcgtcg cttccttcgg cggtagctta tcttccgcaa tgacacgctc
     4561 gacgttctcc accaccacga tggcgtcatc caccagtaac cctatcgcca gcaccatccc
     4621 gaacatagtt aacgtgttga tggtgaaacc gaccgccgac aagatcgcaa acgtcccgag
     4681 aataaccacc ggtacggcaa tcgtcgggat gattgtggca cggaaattct gcaaaaacag
     4741 atacatgacc aggaagacga ggatgatagc ctcaaccagt gttttaaaaa cttcctgaat
     4801 agaaatttcg ataaacggcg tggtgtcgta aggataaacc gtcttcagac ttgcggggaa
     4861 ataggctgat aagcggttca gctcctcttt gactgcccgc gaggtatcca gggcgtttgc
     4921 tccggtagcc agtttgatgg cgatcccggc agcaggtttg ccgttatagc gcgccacggt
     4981 ggaataatct tccgctccaa gttcgacgcg ggcgacatca cgcagcagca cttgcgaacc
     5041 atcttgctga actttcaaca ggattttgcc aaattcttcc ggcgtttgca gacgcgtctg
     5101 cacaatgatc gaggcgttta gttgctggtc tgccgcctgt ggcatgccac ccagttgacc
     5161 accagaaatc tggttgttct gcaccttaat ctgggaaata acatcggaag gtaccagatt
     5221 gtatttattg agtttttgcg ggtccagcca gatacgcatg gcgtactcgg aaccaaagag
     5281 ctgtacgcta ccaacgcccg cggtacggct tagcgggtct ttgatattag acgctacata
     5341 gtccgcgata tcgtactggt tgaggctgcc gttatcagaa ataaacgccg ctaccatcag
     5401 gatattactg ctcgacttat cgacgctaat cccctgctgc tgcaccgctt caggtaatga
     5461 aggcatagcg agttgcagtt tattttgcac ctgaacctgt gcgatatcag gagatgtccc
     5521 agtctcgaag gtcagagtaa tagaggcatt acccgccgca tcactggttg aagacatgta
     5581 catcaggcca tcaagcccat tcatattttg ctcaatcacc tgagtgaccg agtcttctac
     5641 cgtttgcgca tcggcacctg gataggtagc gctgacggta atggtcggtg gcgcaatctg
     5701 cggatactgc gcaaccggta agttcatgat cgccagaccg cctgcaagca tcataataat
     5761 ggcaagtacc caggcaaaaa ccgggcgatc aataaaatag ttagccatga aagccccctt
     5821 aagcctgcaa cgttattgtt tcgattcggt gctgacgttt tcctggctgg aggaaattgc
     5881 tcgtgctttg atacccggac gaatgcgttg caaaccggaa acgatcaccc gatcgccagc
     5941 ctgcaagcca gaggtgacaa cccactggtc gccgatggct ttgctggctt caatttcgcg
     6001 tagctgcacg acatcgtctt tatccagaat gagcgccgct gctttaccct gggcgttgtg
     6061 ggtgacgcct tcctgcggca ccagtaatac attctggcgg ctaccttcat ccactaatgc
     6121 cgtgacatac atgccaggca gcaagtcacc atttgggttg gggaaaatcg cccgtaacgt
     6181 cacggagtcc gtggtttcat ccaccgtcgg gtcggagaat ttcagcgtgc cggtctggct
     6241 gtaacgttta ccattttcca gattgagctg tactggcgta ctgccctgaa cctgtttgat
     6301 ttgcccactg gcgacctctt ctttcatgcg caagaaatcc tgcaccgact gcgtgagatc
     6361 gacataaatc gggtccagac gttgtacggt aaccagcgaa tctgcctgat tagcggtaac
     6421 gagtgcgccg acggtcaccg acgatttccc gctgacgccc gtaatcggcg aggtgacatt
     6481 cgcgtattgc aggttgatag tcgcctgttc aacagccgct ttggcgacgg tgacattggc
     6541 ttctgcttca ttcaactggg tgcgcgcggt gtcgtaatcc tgacggctaa cgtagttggt
     6601 cttcagcaac gatgcctggc ggttaaaggt gatgcgggca ttgctggcgg tagagagcgc
     6661 tttcgccagc gtgcctttgg cggagttcag ctcggcctgt aaaggtgcag gatcaatctg
     6721 atacagcgaa tcgccctggt tcactttatc gccttcgata aagttgcgtt taatgataat
     6781 accgcccacc tggggacgta tctcggcaac ttcataagga acggttctac cgggtaattc
     6841 gctcaacaca ttgaccgaac cgggggagag cgtgacgaca ccgacctcag gcgtcatggc
     6901 ggcggcgttt tccgccgatt tgtcatcgca ggcggtgagc atcgcgccgc agaataacaa
     6961 cggtattaac agctttcttc ttctgttcat tttagtccct gaaaattctt gagaaataac
     7021 ggttctttaa attgcgcgcg aggagagata tgagggattt acagaccagc ggcatagtgg
     7081 ggttgtcagc cagtaaggtg ggatacaggc atagtgatcg acatggtgag gtcaacgaca
     7141 aaaaaaatgt tgccgttctg ccaacagttc ctgccagcat tcgggcaacg aagggttcta
     7201 ctggtggata catataccag gggaataaag tcgttttcgc ctctccttta gtaatcaccg
     7261 ggggtaatga aatcagcatc tgcatccctc gtcatgccag ccatcaattt cagttgctta
     7321 tgtcctgact aaaaataaga tgtgataccc agggtgacga tgtcgctcat acgtcttagt
     7381 ttcagcttgt acataatgtt gaatttctga atctttaatg tttgttgcga aatattgagc
     7441 gtcgacgtga tattgctttc atttttattt ttcagaatca gttgcataat tttgaactga
     7501 ttatgtgaaa aacataattg tgaattctta tggggcaagt gtttaccata agtaatctca
     7561 agaagatttt tgatactttc tttgcgacta acaaaataaa taggcgcttt agcttttagt
     7621 ttactgatgt gataaggtgc caaaacaata attctctcgg catctaattt ctccagaaat
     7681 ttaatcgctt cttcatcaag gatatgattg tgataggtat caatgataac cttgaagggt
     7741 ttattgccta cttttttgat ctctgacagg gaattgattt ttatcatttc gtgattatct
     7801 ttcaactgcc aaaagccctg taaaagaaaa gaatctttcg tcatgagaaa aatcataact
     7861 tgctccttag ccgttatcgt ttcgaacatg tcatcctttt cattatttac atccttgtcc
     7921 gaatcgttgt tcaatatagt aaacgcctat aagaactttg cccttcgtga actaatacgt
     7981 ctattaacac taatcaatac ttcagattta tccatcacgc actccatata ttgatcctaa
     8041 gtaagattcc tggttgttat caatttgtaa atggtaaaca aaaagttaac gtttgcggat
     8101 tactgcccaa gaataagtta tcaataacca gtttgtgatc tctgaagaat attactaaag
     8161 ttaaaatgta atccgattta aatatcgagt ctcctt
//
LOCUS       DAPOZO010000158         8173 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPOZO010000158 DAPOZO010000000
VERSION     DAPOZO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.159"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(162..893)
                     /gene="dnaQ"
                     /locus_tag="ROI21_004451"
     CDS             complement(162..893)
                     /gene="dnaQ"
                     /locus_tag="ROI21_004451"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDX5113287.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            958..1425
                     /gene="rnhA"
                     /locus_tag="ROI21_004452"
     CDS             958..1425
                     /gene="rnhA"
                     /locus_tag="ROI21_004452"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDX5113288.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1422..2144)
                     /locus_tag="ROI21_004453"
     CDS             complement(1422..2144)
                     /locus_tag="ROI21_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDX5113289.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2178..2933
                     /gene="gloB"
                     /locus_tag="ROI21_004454"
     CDS             2178..2933
                     /gene="gloB"
                     /locus_tag="ROI21_004454"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDX5113290.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGITQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3005..4363
                     /gene="mltD"
                     /locus_tag="ROI21_004455"
     CDS             3005..4363
                     /gene="mltD"
                     /locus_tag="ROI21_004455"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDX5113291.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4410..5180)
                     /locus_tag="ROI21_004456"
     CDS             complement(4410..5180)
                     /locus_tag="ROI21_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDX5113292.1"
                     /translation="MTSQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIIVEAHKAA"
     gene            complement(5258..6058)
                     /locus_tag="ROI21_004457"
     CDS             complement(5258..6058)
                     /locus_tag="ROI21_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDX5113293.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6299..7213
                     /locus_tag="ROI21_004458"
     CDS             6299..7213
                     /locus_tag="ROI21_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5113294.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7210..8013)
                     /gene="dkgB"
                     /locus_tag="ROI21_004459"
     CDS             complement(7210..8013)
                     /gene="dkgB"
                     /locus_tag="ROI21_004459"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDX5113295.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLQESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2166 a         1960 c         1993 g         2054 t
ORIGIN      
        1 ggtattctaa ccgactgaac taccgctccg cgttgtgttc cgttgggaac gaggcgaata
       61 ttacggattg cctcaccctt cgtcaacggt ttttctcatc ttttgaatcg tttgctgcaa
      121 aaatcgccca agtcgctatt tttagcgcct tttacaggta tttatgcccg ccagaggcag
      181 cttccgccct ttttctgcac cagatcgaga cgggcttcat gtgccgcaag ctcttcatct
      241 gtcgcaaaaa caacgcgtaa cttacttgcc tgacgcacaa tgcgctgaat tgttgtttca
      301 ccttgttgct gttgtgtctc tccttccatc gcaaaagcca tcgacgtttg accaccagtc
      361 atcgccagat aaacttccgc aaggatctgg gcatcgagta atgccccgtg caacgttcgt
      421 ttactgttat ctatttcgta gcgggcacat aacgcatcga ggctgttgcg cttaccggga
      481 aacattttcc tcgccaccgc aaggctatcg gtgaccttac agaaggtatt ggtcttcgga
      541 atatcgcgct taagcaacga aaactcgtag tccataaagc cgatatcgaa cgctgcgtta
      601 tggatcacca actccgcgcc gcgaatatag tccatgaact catcggctac ttcggcaaac
      661 gtgggcttat cgagcaaaaa ttcatcggca ataccatgta cgccaaaggc ttccggatcc
      721 accagccgat cgggtttgag ataaacatgg aagttattgc ccgtcaggcg acggttcacc
      781 acttcaacgg caccaatctc aatgatcttg tggccttcat agtgcgcgcc aatctggttc
      841 ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa ttgcagtgct catagcggtc
      901 atttatgtca gacttatcgt tttacagttc gattcaatta caggaagtct accagagatg
      961 cttaaacagg tagaaatttt caccgatggt tcgtgtctgg gtaatccagg acctgggggt
     1021 tacggcgcta ttttacgcta tcgcggacgc gagaaaacct ttagcgcagg ctacacccgc
     1081 accaccaaca accgtatgga gttgatggcc gctattgtcg cgctggaggc gttaaaagaa
     1141 cattgcgaag tcattttgag taccgacagc cagtatgtcc gccagggtat cacccagtgg
     1201 atccataact ggaaaaaacg tggctggaaa accgcagaca aaaaaccagt aaaaaatgtc
     1261 gatctctggc aacgtcttga tgctgcgctg gggcagcatc aaatcaaatg ggaatgggtt
     1321 aaaggccatg ccggtcatcc agaaaacgaa cgttgcgatg aactggctcg tgccgcagcg
     1381 atgaatccca cactggaaga tacgggctat caagtcgaag tttaagcctg tggtttacga
     1441 cattgccggg tggctccaac cgcctggcga attcgtggct tgtttttact ctgtttcatc
     1501 ggatttagcg ttaaaggaat agtccgtttc cgggcgacaa taagttgtaa gcaaccaagc
     1561 gcaggaatat gcgcattcaa tagttttcca ccgtgtttgt tccacgggag tacgtggaaa
     1621 cggctggcgt gtagcacttc aaaattcaac aaagagagcc agtccagctg ccgcatcaga
     1681 gtaaacatcc ggctattata gggcgaggtt ttgcgcaata ccggcacaag ttttcgtaat
     1741 cccattaaac tgatgggatt gaagccacta atcaccagcc agccatcatc aatcaatacc
     1801 cgatcggctt cacgcaataa acgatgcgga tcggtgcacc acggcaatgt atgtgccagt
     1861 agacaaacat caacggattt atcggcaaaa ggaagatgaa gtgggtccgc ctgcacctgg
     1921 acgggcattc cttgcgcaga aacattcact tgatgagaaa ccgcgcacgc ttcgcaattg
     1981 atttctgcgc ttaaattgcc aatcttaagc agatgaaaac catacatttt agtgaaccac
     2041 gggttgagct ggcgctccag cgccttgcga taaagctctc cccagggcaa atcgccccag
     2101 caatcaggag ccacgacagt ttgagggact cttgccggtt tcatcacaac cttccgtttc
     2161 acactgagag gtaatctatg aatcttaaca gtattcccgc ctttgatgac aattacatct
     2221 gggttttgaa tgatgaagca ggtcgctgcc tgattgtcga tcccggagac gcagagccag
     2281 tattaaacgc cattgccgcc aataactggc aaccggaggc catatttctc acccaccatc
     2341 accacgatca cgttggcggc gtaaaagaac tggtgaaaaa gtttccgcaa attgtggtgt
     2401 atggtccaca agagacacaa gataagggaa taacacaggt agtcaaagat ggcgaaactg
     2461 ccttcgtttt ggggcatgaa tttagtgtaa ttgccacacc gggtcacact ttaggacata
     2521 tctgttactt cagtaaacct tatctatttt gcggcgacac gctgttttct ggtgggtgtg
     2581 gtcggttgtt tgaagggaca ccatcacaaa tgtatcaatc acttaaaaag ttaagtgcgt
     2641 tacctgacga tacattggta tgttgtgctc atgaatatac cttatcaaat atgaagtttg
     2701 ctttgagtat tcttccgcac gatttgtcca taaatgatta ttatcgtaaa gttaaggagt
     2761 tacgggcaaa aaatcaaata acactacccg taattctgaa aaatgagcgg caaattaatg
     2821 tttttttaag aacagaagat attgatttaa ttaatgtaat taatgaagaa acattattgc
     2881 aacaacctga agaacgtttt gcatggttaa ggtcaaagaa agataggttc tgataaaact
     2941 ttcttgtcat cggctccgtt cgccgttatg atcggtcgtc ttttaagcaa ctattgacac
     3001 acacatgaag gcaaaagcga tattactcgc ctctgtcctg ctcgtgggtt gccagagtac
     3061 cggcaacgtt caacagcacg cacagagcct ttctgcagct ggtcaagggg aagcagcaaa
     3121 gtttacaagt caggcacgat ggatggacga tgggacgtct atcgcgccag atggtgactt
     3181 gtgggctttc attggcgacg agctaaagat gggaattccg gaaaatgacc ggattcgcga
     3241 acagaaacag aaatatttac gcaataagag ctatctccac gatgtaactt tacgggcaga
     3301 gccgtatatg tactggatag ccgggcaagt taaaaaacgt aacatgccta tggaactggt
     3361 actactaccc atagtggaga gcgcttttga tcctcacgca acgtctggcg ccaatgcagc
     3421 aggcatctgg cagatcattc cgagcacggg gcgcaattat ggtttgaaac agacccgcaa
     3481 ttatgacgcg cgtcgcgatg ttgttgcttc aacaactgcc gcgctgaaca tgatgcagcg
     3541 tctgaacaag atgtttgacg gcgactggct tctgaccgta gcggcttata acagcggcga
     3601 aggtcgagtc atgaaggcaa ttaaaacgaa caaagcgcgt gggaaatcca cggacttctg
     3661 gtcgttaccg ttgccgcagg aaacgaagca gtacgtgcct aaaatgctgg cattgagtga
     3721 tattctcaaa aacagcaagc gttatggcgt acgtctgcca acgaccgatg aaagccgtgc
     3781 tctggcgcgt gtgcacctga gcagcccagt tgaaatggcg aaggttgcag atatggcggg
     3841 gatttccgtc agcaagctta agacattcaa cgctggcgtg aaaggctcca cgctgggcgc
     3901 aagtggtccg cagtacgtga tggtgccaaa gaagcatgca gatcaactgc gtgaatctct
     3961 ggcttcgggc gaaattgctg ctgtacagtc gacgctggtt gccgacaata cgccgcttaa
     4021 cagccgtgtt tacaccgtac gctctggcga cacgctttca agtatcgctt cacgtctcgg
     4081 cgttagcacc aaagatttgc agcagtggaa caaactgcgc ggatctaagc tgaagccagg
     4141 ccaaagcttg acgattggcg caggtagtag cgcacagcgg ttggcaaaca acagcgatag
     4201 cattacatat cgtgtgcgca aaggcgattc gctttcaagc attgctaaac gccacggcgt
     4261 gaacatcaaa gatgtgatgc gctggaacag cgatactgcg aatctgcaac caggcgataa
     4321 gctgacgttg tttgtgaaaa acaacaacat gccagattcc tgacaaatca gataatgaaa
     4381 ggcaccgatt cccccggtgc cttttttatt tatgctgctt tatgtgcttc tacaatgatg
     4441 atatcactgg tgaaagagcc atcattctgc aaggcaaaat acgttttcac ctctgtcgat
     4501 gcgctctgtt ggtaaatgcg aatagcgtct actaacgctt ctggcgtacg cattctcgcg
     4561 acccatgaag aaaattccag cggtaactta tctgtaatta aattatcaac tatcagattg
     4621 gcttcattga ttaacgtcaa ccactcaccg ctggcgtagt ttcgtacgtg agaggtatcg
     4681 cgtaatgctt ctaccgtctg taaccagacg tcgcgcactg ggtgccccgg agacattacg
     4741 tccatcacaa tcagcctacc gccaggtttc aatatcctat tcacttctcg cagtgctgca
     4801 ccaacatcat gccagtgatg ggcagaataa cggctgataa caatatcaaa tgcgttatcg
     4861 gcaaatggca gactttcgac ataaccctgg cgggtggtga tatttttcag ttgccgggct
     4921 tcggcagctt gtgccacgac atccagcatg tgggcagata agtcatacgc caccaccgcg
     4981 ctcacgttct gcgcagcgac aaagctggca tgccctgctc cgcagcccat atcaagtaca
     5041 cttgcatcag gataatcagc cagacgcacc gccaggcgct gtaaatcccg tccggatgca
     5101 tgtacggtac tagtcagata ttcgctagct tgagagctaa actgcttttc tacgtggtca
     5161 tggtgggatt gtgatgtcat tgcgctgtcc ttttgttgtt tgagtataaa agggcagcgc
     5221 caaacgttgg tctgccctgt ggcagacctg acatacctta tttatcaggc ttgccgggac
     5281 tgaattcaac gagtagcgga ttgtgatcgg aagcgcgcgt aaccagtaca gaagcttcgc
     5341 tgacgttcag accacggtaa aaaacaaaat cgagcgggcg accaaacgcc cgacggcgct
     5401 gatcgtcggt aaaacgcacc tggcgcagcg acatttcccg cgcaaagcga tataacgcgt
     5461 tcatccttct acggctccag gcattgaaat ctcccgccat aatgaccggg ccgctgtgat
     5521 gagctatctg atcgccaata ggaagtaact gcttactata aacatccacg cccagactga
     5581 agttgacggc gtgtatatta accaccatca acaggcgggt gtcaggtaat ggatagaccg
     5641 tcaccagtgc cgacttcgcc agacgcaaaa tgggttctcg ttcacgtaac gggcagcaat
     5701 acactggatg tgccgccgaa agggtcatta cgccagaagg atgttgtggc agcacgaaag
     5761 cgggtacctg atcggcggca agatagttag cggtcgcaaa ctgtactaac tctggcgttg
     5821 tctgcgcttc ctgcaataac accagatgtg catctttgcc gtagttcttt aataccgaca
     5881 accattcagc gcgttgctgt ttgtatatgt tccacaccag gatccgaata cgctcttcgg
     5941 tacttaacgg ttctccgggt ggtaatgcct ggccgatgct cgcaaaagac cccggcggta
     6001 agatcctttc cgcaggttgt ccggcaacat agcgcatggc ataggtgttt tttcgcactt
     6061 ttgactttga aacctctatt acgtcaaccc ttccgcactc gggagggttc tactgttata
     6121 gggattttag ctcactcttt caacgagcaa ttgtatattg ttatgtaagc aagtgcttac
     6181 accggtcaga aatttgctca ttattgagaa cattaaatcg gatgacatat cacgcatcct
     6241 gatggtttaa tgacaaataa attcaaaata tctcatctat tttgcaacag gagcaaaaat
     6301 gaaagccacg tcggaagaac tcgccatttt tgtttcggtc gtcgaaagcg gcagctttag
     6361 ccgggcagcg gaacaattag ggcaagcaaa ctcagcggta agccgggcgg tgaagaagct
     6421 ggagatgaaa cttggcgtta gcctgcttaa tcggaccacg cggcaactta gcctgacgga
     6481 agaaggcgag cgttattttc gtcgcgtaca gtcaattttg caggagatgg cagcggcaga
     6541 atcagaaatt atggagacgc gtaatacacc gcgtggactg ttgcgcatcg atgccgcaac
     6601 tccagtagtg ctgcactttc tgatgccgtt gattaagcct ttccgtgaac gctatccgga
     6661 agtcacttta tcactagtct cctccgaaac gattattaat ttgatcgaaa gaaaagtgga
     6721 tgtcgcgata cgcgccggta cgttaacgga ttccagctta cgtgccaggc cgttatttaa
     6781 cagttatcga aaaattatag cctcccccga ttatatttcc cgctatggga agccagaaac
     6841 gatcgacgat ttaaagcaac atgtttgcct gggattcact gaacccgctt ccctcaatac
     6901 ctggccgata gcctgtagcg atggacaatt acatgaggtg aagtacggtt tgtcatccaa
     6961 tagtggggaa acactgaaac agctttgcct gagtggcaac gggattgcgt gtttgtccga
     7021 ctacatgatc gacagagaaa tcgctcgcgg agaattggtg gagctaatgg cagataaagt
     7081 gttgccagtg gaaatgccat tcagtgcagt ctattacagc gaccgtgcgg taagtacgcg
     7141 catccgggct tttatcgatt tccttagcga gcatgtaaaa acagctcccg gaggagctgt
     7201 cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc ggtcgttgca
     7261 atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt gtgccttaag
     7321 attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac cttcccccat
     7381 agcccacgcc agaatcactt gtgccggagt cgcattgtgt ttagctgcga tacgagcaat
     7441 aacctcatct ttcagggcct taccatacgc cagcgtcata taggaagtaa tatggatgcc
     7501 gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga gttcaatctg
     7561 gttagtagcg atgttttcag caccaacagc agcaatcgct ttttccatca atgggatcgt
     7621 gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca gcagcgcctg
     7681 cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta gcgtcagatc
     7741 aacataatca gtacgcaatt tttgcaggct ctcttgcaga ctcgggatca atttgtcttt
     7801 gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg gcacgccact
     7861 ttctgcaatc gcctgaccta cggcagcttc gttatcatag atttgtgcgg tatcaattgc
     7921 gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt ctttgagacg
     7981 gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg
     8041 ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa atgcagagga
     8101 tttttgcgat tctggcaata atagatatac agacgaaaaa aaacctcgag cgttaactcg
     8161 aggcttctta ata
//
LOCUS       DAPOZO010000159         8056 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPOZO010000159 DAPOZO010000000
VERSION     DAPOZO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.160"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(148..1578)
                     /locus_tag="ROI21_004460"
     CDS             complement(148..1578)
                     /locus_tag="ROI21_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDX5113296.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANGK"
     gene            complement(1788..2936)
                     /locus_tag="ROI21_004461"
     CDS             complement(1788..2936)
                     /locus_tag="ROI21_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5113297.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDATSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            3251..3877
                     /locus_tag="ROI21_004462"
     CDS             3251..3877
                     /locus_tag="ROI21_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDX5113298.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKTQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(3913..4776)
                     /gene="dmsC"
                     /locus_tag="ROI21_004463"
     CDS             complement(3913..4776)
                     /gene="dmsC"
                     /locus_tag="ROI21_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDX5113299.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(4778..5395)
                     /gene="dmsB"
                     /locus_tag="ROI21_004464"
     CDS             complement(4778..5395)
                     /gene="dmsB"
                     /locus_tag="ROI21_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDX5113300.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(5406..7850)
                     /gene="dmsA"
                     /locus_tag="ROI21_004465"
     CDS             complement(5406..7850)
                     /gene="dmsA"
                     /locus_tag="ROI21_004465"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDX5113301.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
BASE COUNT         2185 a         2142 c         1860 g         1869 t
ORIGIN      
        1 ttcagagctc tatttctaac gcccttacag cacagcttcc aaatgtatta cccaaaccgc
       61 cagtatatgt cctgcggctt acgcgcattt ctcgattatg taaaaacggg tcagatctgc
      121 cagagtcagc gtcaccgacc acaataatta tttaccattc gctttacgcg ccagcagcgg
      181 gaatatcagc cctaatccta ccagcacaaa cggcgttgca acattcagcg ccaactggaa
      241 tgtccactct gcggtgaagg cttccatttt cgggaaaata ccagtcaggc aggcaaaagc
      301 ggtaaaggca aaacaccaaa ttccgacggt cattgccagc ggcttattac gaataaagac
      361 atattctggt ttatatttct gcgccaggcg aacgactgca ataaatgcaa caaataccca
      421 taaataacgc agcggcatca ctaccgaatt aaggttcaac agccatttat agagattgtt
      481 catatcacca atgccgagag tcggtagcat tatcagaatc gccaccagca ccagggtcag
      541 aaaatagcca ttaacgggcg taccagaagc gttggtacga cataaactgg ctggaatata
      601 tttgctgtca gcatcgccta ataacacttt aagcggcgca tcaatcgaga ataccagcgc
      661 agctacttgt cccagggtat tcgcaatggc ataaatcacc attaaagtat tacccatgtt
      721 gtaatactcg cccagcttct gaaaggcgta atactgaccg ttagtcatta agtcatccgg
      781 gatattacgc gagtcaaaca tcatccccat cgccagtgag cccagaatgg cacaaaccgc
      841 aaccatcacc gccaggcata acatcccttt tggaaattct tttcctgggt tgcgcgtttg
      901 attaacgtaa ggagaaatct tctctgctcc gccaaccgcg aaaaccagca ttgaaatagt
      961 ggtaatgtag gtaaagtcga tatgaggaat gaacgtttcc caggtaatgt ttgtggtcgc
     1021 aatatgcact tcagtaattg caggcgcggt taccgccatc gccacataca ggagcgacat
     1081 aacaaacatc gcaatccctg ccacagaacc gacgattttc agcgatttca taccgcgtga
     1141 agcaacccac ataaagaaga taaacagcac cagcgttaac ccctgtaacg ctacgactga
     1201 atattctttg attaacgaac cgtcgccttt catcgcccaa ccgagcgcaa tcagtattgc
     1261 ctggggtttt tgtgccagat agggaatatg cactacccag taggtccacg cggcgaggta
     1321 agccagtccg ggtcccatcg tgtgtttaat ccaggtactg accccgccct tcccatcttt
     1381 gaaggtcgag cctaactgac caacaattag cgcataaggt gtgaaataga gcgcaaagat
     1441 aaacacccat gaaaaaacaa ccaccagccc ctggttggcg tagttattaa caacgttgcc
     1501 aaaaccccag acagtgataa aagacatcag cgcaatgttg taccatcgca gctgtttttc
     1561 ctgaacattc ccagccatag acaaatcctt cgccaaaatt aaaggggcat ctgtaaaacg
     1621 agggattatg cgcggcaaat aagaagtgcg gaaatagcga aatgcaaaat tataatctgc
     1681 tgcacatttt atttttaaac gaataaaatt tggaataaag caagtcagat agaagaaaca
     1741 aaaaagcaga gtgtgaaaca gtaagttcac actctgcttg aattcattta cacgtgagca
     1801 acgggtttcg gcgtatgacc ggcgttgcga agcagcatca gcaaatagat aaacgatacg
     1861 ctggcgatca tgataaacaa taaattatcg gagaaattct gcattagcat ggcagtaaat
     1921 gacgggccaa gcagacttcc cacggtatag ctcaacagta aggcctggtt catcgccacc
     1981 agttgatgat gttcaacttt ctcgcaagcc catgccatcg ccaccggata tagcgtaaag
     2041 ccagcggcac cgaggatgaa taacgctggg gccatcgccg cctggctaag catcgcgata
     2101 ctgccgagaa tgacgacaaa gacctgaaca cgcaacacca gcagtcgacc aaacttatcc
     2161 gccagacgtc caatcggcca ttgtccaagg atacccgcac tgaccagtac cgccatccag
     2221 aaaccaatgc tggcattgct cacccctttg tgattgaggt acagcggcat caggccatat
     2281 agagatccca gaacgattcc tgagataatg cagccattca cgccaagccg cgcctgacgg
     2341 agttttagca ttgacgtaat tgacgtcgca tcatggtttt cagcctgctg attcagcaca
     2401 cgcgtaaaca acaacggtaa gatccctgcc aacgtcaaac ctgtaaccca cggcaatacg
     2461 gacatcagct cggttgaaac tttgctgacc agtaactggc ctaaaaacgt tcccacgtaa
     2521 taaaccatca tatacgcagc aagcaaacgc ccacggttac gtgacgtccc actgcacatc
     2581 agcgcgctct caacaaccac ccaaatcatg gcacagccga cgccggcgac aaaacgccaa
     2641 gccaaccagc tccagaatcc aatcattaga ccaaggccgg cacagccagc ggcaaaaatg
     2701 aaggaggcca gataatagct gcggttaaag ccaatgcgct taatgacata ccctgtcagc
     2761 aatgtaccga ccaggttgcc ggtaaaatag gatgagctga caacgcctac ctgccatgtg
     2821 gacatgtgtt cctgggcgag ccaaagcggt acgagtgtat ttaacactgc gatcgccaga
     2881 gtcaacaaaa gcaggccaga cagcaaaagc atgacaggcc gggtatacgt ggacatgaat
     2941 taaaaccgtg aggaagttca aatttcatgc gcatcatgcc accgccaaaa acattgtcaa
     3001 tgggtgaaaa tacgcgcata tcgcagcgga ggtattatcg atttatatta ctcattcata
     3061 acgctaatgc tggagagacg attaatctca tctgattgtt tttattgatc taatacaaat
     3121 ttcatacgct tcgttcagtc gaaaaatttc atggtcacag caaactcata tttcccgtct
     3181 atgctttcag agcaatgcat cgcgtctgac cgatttcaga cgtaccactg actctcgaaa
     3241 ggagaagagg atgaccaaac cgtatgttcg tcttgataaa aatgatgctg ccgttttgct
     3301 tgttgatcac caggctggtt tactttccct tgtacgggat atcgaacccg ataagtttaa
     3361 aaataacgtg ctggcgctgg gcgacctggc taagtatttc aacttaccta ctattcttac
     3421 caccagtttt gaaaccggtc ctaacggccc attagttcca gaactgaaaa cacagttccc
     3481 cgatgcacct tacattgctc gcccgggaaa tattaacgcc tgggataacg aagattttgt
     3541 aaaagctgtc aaagcgacag gtaaaaaaca gttaattatt gccggtgtgg taaccgaagt
     3601 ttgcgtggca ttcccggcgc tttcagccat tgaagaggga tttgatgtat ttgtcgtgac
     3661 tgatgcatca gggaccttta atgaaattac ccggcattcg gcgtgggatc gcatgtcgca
     3721 agctggtgcg caattgatga catggttcgg cgtggcttgc gagctgcacc gtgactggcg
     3781 taatgatatt gaaggactgg cgacgttgtt ctctaaccac attcctgatt atcgtaattt
     3841 gatgaccagt tatgacacat taacgaagca gaaataaaag agaaccgggg tcgaaacccc
     3901 ggttgttact gattagcttg cgacggccat ccccacggtc atatgcaaac catagaatac
     3961 gccgcggccg attaattctc ctgccagaag cagaatgaat gagacggata gtagcggaac
     4021 cgcaggctga taacctttta gctgtggtgc aatccacaaa cacagggcaa cagccaaaag
     4081 cacgatccgc caggacatca gcgcaccata gtccggcacc agcgctgcgg cctgctgcac
     4141 agaactatga atagtcgcca gctctgcgcc ctgcatcact gacatcacgc cactcactac
     4201 cagtgccagt acagaaatgg ctggcagcag acgcatcgcc cagccatcga cgccagccag
     4261 actcagcaac aggtaaccca gtaacgggcc gcccataaac atcgtcagga agaagcccat
     4321 cggcgtccag atgctgtacc aggtcggaac ggtatcaatg ctgttataca cacgcaccat
     4381 catccagaca aagatgacgc caagaaccat cgtcactatc agccagagcg tacgcaatgc
     4441 cggtgacagc tttttcagca ttgccagcaa ccagccgatg ccaccaacag caaaaaagat
     4501 cgaaccgctg gcgatttcgt tactgagtgc tgaagcccct acccggttga gcgagttaaa
     4561 ggcgcgcatc ggtgaaccaa gatgaagcat agaggcgata aagccaatgc ccatcagcac
     4621 ccataaacca aacatgcagg caataacacg ctgctgggct tctgctcgca ggtcgccttt
     4681 gagcagcgcc aaagccagaa cgataaaacc acccgctaca cattgcccga agaccgtgaa
     4741 gatcatcagc ggccattcat gccatccact tcccatctca cacctccttc gggtttgcca
     4801 gatagccagt ggtatccccg gtcgggcggc tgttggcatt gggtttgatc acaatattcg
     4861 gtttggtaaa gtgagctcgc ggcaacggcg caacggccgc cagatcgcca tgttttttac
     4921 gcagctcgtc gatagggccg aaatccagcg cccgcagcgg acaagattca acacagatcg
     4981 gctttttacc ctccgcaaca cggtcataac aaccatcgca tttggtcata tggcctttcg
     5041 tttcgttata ttgcggtgcg ccatacgggc aagccatatg gcagtagcgg cagccaatgc
     5101 acacatcttc atcgaccaca acaaaaccat cttcacgttt atgcatcgca ccgctcgggc
     5161 agactttagt acaagccgga tcttcgcagt ggttgcacga aatcgacaga tagtaggcaa
     5221 acacgttctg gtgccagaca ccgttatctt cctgccagtc gccaccagca tattcataaa
     5281 tgcggcggaa gctgacttcc ggcgtcagat ctttatagtc tttacaggcc agctcgcagg
     5341 ttttgcaacc ggtgcaacgg ctggaatcaa taaaaaatcc atactgggtt gtcatcggtt
     5401 actccttaca ccttttcaac ctgaacaagg tttgtatgtg acggattccc cttagcgaga
     5461 ggagacggac gttgagtggt cagtacgttg atacaacctc ccttatcgac acgttttgca
     5521 tccgggtcat accaggcacc ttcacccagt gcgaccacac ccggcatcat tcgtggcgtc
     5581 actttcgcct caatatgaac ctcaccacga tcgttaaaga tcctgacttt atcgccgttg
     5641 ttgataccgc gtttttgggc atcaagcggg ttgatccaca tttcctgacg gcaagccgct
     5701 ttcagcacat caacgttgcc gtaagttgag tgaacgcgag atttatagtg gaaacccgta
     5761 agctgcagcg gatactgttt gttcagcgga tcctgataac tttcaaagcc cggcgtgtag
     5821 atcggcagtg gatcgatcac atcgccttct ggcaattccc aggtagcggc aatgtcagcc
     5881 agcgcctgcg aataaatctc aattttaccc gatggcgtgg tcagtggatt tgcctgcgga
     5941 tcttcacgga aggctttata agcaacgtga tgcccttgtg ggtcgcgctt tttaaagatc
     6001 ccctgcttgc ggaactcttc aaacgttggc agttcaggaa tcgcttcccg cgactgggca
     6061 tacagatgcc gcatccactc ttcctgggta cggccttcag taaactgttg ctcaacgcca
     6121 agacgttttg ccagttcgct ggtcatttca tagatggtct tacattcaaa gcgcggttta
     6181 atcacctgat cgttgaaaat cacgtaagac atattcccgc aggatgcatc cagtgcaaag
     6241 tccatctgtt cggaagcggt gcagtcaggc agcaggatgt cagcatattt cgccgatgag
     6301 gtcatgtggc agtcgataac cacaatcagc tcgcacttct tatcatcctg aaggatttca
     6361 tgggtacggt tgatttcaga atgctggtta atcaggcagt taccggcata gttccagatc
     6421 attttgatcg gcacatccag cttatctttc ccgcgcacac catcacgcag cgctgtcatt
     6481 tccgggccac gttcaatggc atcggtccac ataaacatag aaatgctggt ctggatcggg
     6541 ttttcgaggg tcggcatacg cacaaacggt aagctgtatg aaccttcacg cgcgccgctg
     6601 ttccctccat taataccaac gttaccggtc agaatcgcca gcatcgagat agcacgggtt
     6661 gcgatttcac cgttagcgtg acgctgcggg ccccatccct ggctgataaa cgccggtttg
     6721 gtactaccga tttcacgagc caatttgatg attttgtctg ccggaacacc ggtgatttgc
     6781 gaggcccatt ccggcgtttt agctacgcca tctggccctt cacccagaat ataagcttta
     6841 tagtgaccat ttttcggcgc actggcaggc agtgttttct cgtcgtagcc aacgcaatat
     6901 ttatcgagga atgcctgatc caccaggttt tcggtgatca tgacatacgc cagaccgtta
     6961 accagtgcgg catctgttcc cggacgaata gggatccact catcttcgcg cccggctccg
     7021 gtgtcggtat agcgcggatc gatgatgatc atgcgagcat tagatttctg acgcgcctgt
     7081 tcaagatagt aagtcacccc accgccactc attcgcgttt cgccagggtt attaccaaac
     7141 agcactacca gtttactgtt ttcgatatcc gacgggctgt taccatctgc ccagccgccg
     7201 taggtatagt tcaaaccttc cgcaatttgc gcggaagagt agtcaccgta atggttcaga
     7261 taaccgccgc agcagttcat caggcgagcg accagggtat ttcccggcgg ccaggagcgg
     7321 gtcatggtgc cacccagcgt accggtgcca tagttcagat agatggattc gttgccgtac
     7381 tctttgatca ggcgctgcat attggtcgcg atgatgtcgt aggcttcttc ccagctaatg
     7441 cgctcgaatt tgccttcacc gcgcgccccg actcgtttca tcggatattt caggcggtcc
     7501 ggattgtaga cacggcgacg catggaacga ccacgcagac aggcgcgcac ctggtgcagg
     7561 ccgtcatagt tgtcatcgcc agtattatcc gtttcaacat atttgatttc accgtccacg
     7621 acgtgcatac gtagcgggca gcgactacca cagttaactg tacaggcgct ccagataacc
     7681 ttttcgtctg attttgttgg aatggcgcta tcgacagcgt gcgcaatccg actaaaaggt
     7741 aatgttaacg cgctgctggc cattgccagg ccgccgatcg ctgtcgtttt taccaaacca
     7801 cggcgactca cctcagcagc caataccgca tcagggattt tcgttttcat aatggctcac
     7861 tcaagcttgc tcacgattga ttccagtaac aaaccgtatt gtgtaattat ttctatagca
     7921 ggtcgctata taggcttgta tacatcgaat aatttgaaaa tcaaagagat aggtagattt
     7981 tcactcgaaa gtagtattac gtgtaagtga ggagtaatta ttgttcggta tcaaagggag
     8041 acaaaaaaag cgcccg
//
LOCUS       DAPOZO010000160         7939 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPOZO010000160 DAPOZO010000000
VERSION     DAPOZO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.161"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..649
                     /locus_tag="ROI21_004466"
     CDS             <1..649
                     /locus_tag="ROI21_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDX5113302.1"
                     /translation="KKTNYTDKQYLHVKDNICNIEIYNKSFNHKLILTKGVVTFITGE
                     NGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNVLYVPQDLDLMPGTIEENI
                     LHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQKQKLGVSLTSGKNVDLII
                     FDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNIPKAQIIDITKHEV"
     gene            652..1545
                     /locus_tag="ROI21_004467"
     CDS             652..1545
                     /locus_tag="ROI21_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HDX5113303.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLE
                     KDILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVD
                     RCQSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKW
                     KVYNPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHI
                     SFTVIRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFA
                     ENMLKAQSRTYRYKKIKLEHI"
     gene            complement(1570..1932)
                     /locus_tag="ROI21_004468"
     CDS             complement(1570..1932)
                     /locus_tag="ROI21_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HDX5113304.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVI
                     YKKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIEN
                     WIKVNGGWKMQFISYEKI"
     gene            complement(1929..2168)
                     /locus_tag="ROI21_004469"
     CDS             complement(1929..2168)
                     /locus_tag="ROI21_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="HDX5113305.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILK
                     RVIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
     gene            complement(2879..3112)
                     /locus_tag="ROI21_004470"
     CDS             complement(2879..3112)
                     /locus_tag="ROI21_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HDX5113306.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3119..3637)
                     /locus_tag="ROI21_004471"
     CDS             complement(3119..3637)
                     /locus_tag="ROI21_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HDX5113307.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTV
                     SGGTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVI
                     PYQGKDWVQLTE"
     gene            complement(3615..4325)
                     /locus_tag="ROI21_004472"
                     /pseudo
     CDS             complement(3615..4325)
                     /locus_tag="ROI21_004472"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4315..4638)
                     /locus_tag="ROI21_004473"
     CDS             complement(4315..4638)
                     /locus_tag="ROI21_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDX5113308.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQG
                     ARDDC"
     gene            4703..4894
                     /locus_tag="ROI21_004474"
     CDS             4703..4894
                     /locus_tag="ROI21_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HDX5113309.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            6259..6957
                     /locus_tag="ROI21_004475"
     CDS             6259..6957
                     /locus_tag="ROI21_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113310.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPE
                     EADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLG
                     AYLHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDY
                     AVRYSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGC
                     SGKGCGAGCSCNSR"
     gene            6996..>7939
                     /locus_tag="ROI21_004476"
     CDS             6996..>7939
                     /locus_tag="ROI21_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDX5113311.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNK
                     DSIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQ
                     EHFLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIE
                     FIIIMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRK
                     RVIQAVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIM
                     LATFLFQVFLPLNRIGYLFRQIKMARTEIDLYCAEIND"
BASE COUNT         2408 a         1844 c         1461 g         2226 t
ORIGIN      
        1 aaaaaaaaca aattacacag acaagcaata tcttcatgta aaagacaata tttgcaatat
       61 tgaaatatac aacaaatcat tcaaccataa attaatatta acaaaaggcg ttgttacctt
      121 tataaccggt gagaatggtt cgggaaaaac aactattgca aaaattctct ctggcaatat
      181 aacaacagaa gaaaacacaa taaaaatcaa tgggatacaa caagaaaaag caaattctcc
      241 ttttgttaat gtcttatacg ttcctcaaga tctagatctc atgcctggaa ctatagagga
      301 aaatatatta cattattccg gcattaataa tttaaataca attaaaaagc tgctacacag
      361 atttaagttc gacaaaccac tagattatga gatcaaaggt tacggacata atttatctgg
      421 tggacaaaaa caaaaattag gagtatctct tacatctgga aaaaacgtag acttgattat
      481 tttcgatgaa ccgacaaaag ggtttgattc acttggtata aaaataatca gtgattatat
      541 aaaagaggaa aacaaggaaa agcatatcat tgttatatct catgatgaac aacttataaa
      601 taatatacct aaagcacaga ttatagatat cacaaaacat gaggtttaaa aatgaataat
      661 cttataaaaa aggaaatcat agaaaaattt aagaaatata atttccagaa atatccgttt
      721 gtattcacgg atgttaatta taaaaactta atcaactgga atgatcttaa taaattgctt
      781 gaaaaagata tattgcacta tcctagagtt agaatggcaa atgacaactt tcccgaaatt
      841 agggggtata aaggatttat aagatacaca tatagccaaa caggcgatag aacaccacat
      901 ataaatcgcc atcagctata taaatgctta cgtgatggcg caacccttat agtagatcga
      961 tgccagtcat tctttgaatc tgtagatgaa agcagactat ggttatctaa agagttagaa
     1021 tgtacatgta gtgctaatct atatgctgca tttacagcaa caccaagctt tgggcttcat
     1081 tttgacaatc atgatgtaat agctgttcaa attgagggaa taaaaaaatg gaaagtttac
     1141 aaccctacct attcataccc tctcgaagat gaaagaagct tcgattatct accacctaat
     1201 acctccccag attatgagtt tgatataacc ccaggtcagg ccatatatct tcctgctgga
     1261 tactggcaca atgttacgac tcagagcaaa cactctcttc atatatcttt tacagttata
     1321 agacctcgtc gattagaact atttaaaaca ttatttgatg aattaaaaaa caatccatat
     1381 atgcgggaac ctatagagca tggtgattca ttatctgata aagaaaaaat aaaaactata
     1441 ataactaatg ctataaatag ttttgatatt cagtttgcag aaaacatgct taaagctcaa
     1501 agcagaactt atcgctataa aaaaataaaa cttgaacata tataaccatt aatctaaggg
     1561 ggcttcccct tagatttttt catacgatat aaactgcatt ttccatccac cattaacctt
     1621 tatccaattc tcaataaaag ccattaatat atttccatca ttttgcttta tttcagcttt
     1681 ccctgaaaca caagcaatgc ctttaaactc acgcattacg acatcatatt catatctttc
     1741 tatgctaggt aaaatcctcg ctggacgcat atcaatcttt cgtaactgat gttttttata
     1801 gataactttt tgaccatttg ttgaaataaa ccaatcagct tccaagtaat ctagcatttc
     1861 agtattacct gaataaaatg cattatacca ttcttttctt attagattta agtctgattt
     1921 aaaattagtc acactacctt cctcatatct aattcctcct ctaatctatc cccaacattc
     1981 cttactgatt ttatagcatc atcaatagcc acattatata tgactgggat tactctttta
     2041 agaatctcat caagtaattc ctctgattca aactgtccaa ggcatatatc atgttcatct
     2101 ctgatttttt gtgaaataaa ttcagctagt cctttatata tgtttttttc aatatcagaa
     2161 attttcattc tttatgaagc ctcacaaata aattaataat ataacatttt tcctatcata
     2221 ggaaaattac aactcaacca tactgcaacc tggaatttcc caagcaagca tatttaaata
     2281 agtgcaggat accacctcaa atcattctct aaggaacaag ataatttacc ataatccctt
     2341 attgtacgca ccgctgaaac gcgttcggcg cgatcacggc agcagacagg taaaaatggc
     2401 aacaaaccac ccgaaaaact gccgcgatcg cacccggtaa attttaacca catgcatagc
     2461 tatgcagcca tgtgaatcac gcaggaattg ccagccagag acagctgaaa cggatttttt
     2521 tcatgctttc ggaagcgaag aagacgggga ccggagcggg aaaacagaag ttcaggggaa
     2581 tgagcgcgat ctggcaatag aggcaaaaca cagcaacaaa agacacacca gaatcgcgcc
     2641 cgtatgcgtt ttaacgcgtt cctgtgtttt cagcaaggtc tgactgaagc gcgttcccgg
     2701 tgcccccgaa ccaaccatta tcccaaaaaa tgcaccggaa acgcgctgac aacattttag
     2761 cctgtctctg attaccatcc cagcgaaggg ccatcctgtg tccgttcctg tatttccagc
     2821 tggcgctcac gctccaggga taactcatgt tcgcgttcct gtatcagccg ttcatccctt
     2881 atctcctgtt ctcgctgtat aactggctca agcgttctgt ctgctcgctc aagtgctgca
     2941 cctgctgact caactgcatg acccgctcgt tcagcatcgc gttgtcccgt tgcgtaagcg
     3001 aaaacatttt ctgcaattcc acgaaggcgc tctcccattc gttcagccgc tgcatatagt
     3061 cctgttgcag ttgctctaag gcgttcagca aatgtctttc cagctccgtc actctgtgtc
     3121 actccgtcag ttgtacccag tccttcccct gatagggaat caccgttgcc gatttcccct
     3181 gagggataac cagaaatttc cttctcccgt cctgcacaaa ctccacgccc catgtcttcg
     3241 cgttcagttt ctgcaatgtt tcttcctgta tcctgatttc ttccaggttc gcctgtatca
     3301 tcccgccaag ataccagagc gtcccgccac tcacggtaaa caggaaaagg accatcccca
     3361 gtaacatcat gcccgtattc cctgccagct ttaacacgtc cttcctgtgc tgcatcatcg
     3421 cctctttcac cccttcccgg tgttttttca gtgattcctc tgtcgaagct gtgaacaggg
     3481 ctatagcgtc tttgattttc gtctcgtttg atgtcacagc cttgcttaca gatttttcga
     3541 gcttgctgaa ctcgtcgttc agcattgtct ctgtagattc ggctctctct ttcagctttt
     3601 tctcgaactc cgcgcccgtc tgtaaaagat tgctcataaa atgctccttt cagcctgata
     3661 ttcttcccac cgttcgggtc tgcaatgctg atactgcttc gcatcaccct gaccacttca
     3721 agccctgcct ctgtgagcgc ctgaatcaca tcctgacgac cttttatctc cccgacatga
     3781 taaagagcgt ctatcccgcg tgtgacgctc tctgcaagcg cctgtttcgt ttccggcagg
     3841 ttatcaggga gagtcagtat ccggcggttc tccggggcgt tggggtcatg cagcccgtaa
     3901 tggtgattca ccagcgtctg ccaggcatca attctcggcc tgtctgcccg gtcgtagtac
     3961 ggctggaggc gttttccggt ctgtagctcc atgttcggaa tgacaaaatt cagctcaagc
     4021 cgtcccttgt cctggtgctc cacccacagg atgctgtact gattcttttc gagaccgggc
     4081 atcagtacac gctcaaagct cgccatcacc ttttcacgtc ctcccggcgg cagctccttc
     4141 tccgcgaacg acagaacacc tgacgtgtat ttcttcgcaa atggcgtggc atcgatgagt
     4201 tcccggactt cttccggatt accctgaagc accgttgccc cttcccggtt tcgctccctt
     4261 cccagcaggt aatcaaccgg accactgcca ccgccttttc ccctggcatg aaatttaaca
     4321 atcatcccgc gcgccctgtt ccctgacggc cagacgcagc cgacgcagct cctccccgat
     4381 ggccatcagc gcagccaaca cctgaacccg atcaccggaa gaccactgcc cgctgttcac
     4441 cttcctcgct gtctgattca ggttattccc gatggcggcc agctgacgca gtaacggcgg
     4501 tgccagtgtc ggcagcttcc cggagcgtgc aaccggctca cccaggcaga cccgccgcat
     4561 ccataccgcc agctgtttac cctcacagcg ttccagtaac cgggcatgtt catcatcagt
     4621 aacccgtatg gtgagcatcc tctcgcgttt catcggtatc attaccccat aaaacagaaa
     4681 tcccccttac acggaggcat cagtgactaa acaggaaaaa accgccctta acatggcccg
     4741 ttttatcaga agccagacat taacgctgct ggagaaactc aacgaactgg acgcagatga
     4801 acaggccgat atttgtgaat cacttcacga ccacgccgac gagctttacc gcagctgcct
     4861 cgcacgcttc ggggatgacg gtgaaaacct ctgacacata cagctcccgg agacggtaac
     4921 agcttgtctg tgagcggatg ccgggagcag acaagcccct cagggcgcgt cagggggttt
     4981 cggggcgaag ccctgaacca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     5041 cggcatcagt acgaattgta tggaaagcgc accatgtccg atgtgaaatg ccgcacagat
     5101 gcgtaaggag aaaatgcacg ttccggcgct cttccgcttc ctcgctcact gactcgctac
     5161 actcggtcgt tcggctgcgg cgagcggtgt ctgctcactc aaaagcggag atgcggttat
     5221 ccacagaatc aggggataac gccaaaagaa acatgtgagc aaaaaacgag aaccaggaaa
     5281 aggctgcgcc gttggcgttt ttccataggc tccgcccccc tgacgagcat cataaaaatc
     5341 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5401 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5461 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcggct
     5521 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5581 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacaaagcgc
     5641 cactggcagc agccactggt aactggacaa ggtggattga gatattaaga gttcttgaag
     5701 tggtggccta actgcggcta cactagaagg acagtatttg gtatctgtgc tccaccaagc
     5761 cagttacccg gttaagcagt ccccaactga cttaaccttc gactaaaccg cctccccagg
     5821 cggttttttc gtttactggc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5881 tttgatcttt ttgagatccg cccactcctg gaacctacaa cacatgtgta aaacgtcaat
     5941 ggagggggct attatcggac tgcaacacat ttcgtacagc ctttacactc ggtgaattag
     6001 cggccctaga tgcatccact cattcaacca actcaattct cttctcttag agtgggaaaa
     6061 ttttgttttc cttgcagccc ctacccgtaa aaactggctg caaaaacctt gtattactaa
     6121 tggatcctat aaccattaaa aaacttgatt actatctcat ttatagaatt aggcatattg
     6181 acacaagcac aaatttaaca ataccataac aaatgataac tattctcatc tacattcaaa
     6241 tatataattg ggggtgttat gctacaacat aagatgaaca gcagttctta tgcaaaagtt
     6301 cataatgtta gctcattaga agatatcatg agttatcaca atgatgatgt tcttctaaaa
     6361 tttcgtaaag aatggaatgt aacaccagaa gaagctgatg atatctttaa tgaaacaaag
     6421 aaattcatct ggctagcctc aacatgccta acggaatgct acaatataaa agttcacgag
     6481 caattacaaa ttatagatga aatgtggcat acgtttattc aatttacaga tgcttatacc
     6541 agcttttgtg aaaaatatct tggtgcttac ctccaccatt atccaaatac aaatgacatg
     6601 ctaaaaaatg agataaggca tgttaatgag catggtataa cattccaaga atatcgtttt
     6661 aacgaatata aaaatcaaat tgaaaaaatc gctttttacc taggtcatga aactgtcgca
     6721 aagtggtatg gtgattatgc tgtaagatac agcataaaga acattaatac tataagaatt
     6781 ccaaaagaat ccatatccag tgattcttac atcgaaaaag taaagagtat cactcacctt
     6841 ccagccgcag aatttgtaaa aataataatg cgaaaagatg tttggaatga taatggttct
     6901 gtttgtggtt gcagcggtaa aggatgtggc gctgggtgct catgtaattc tagataacat
     6961 aaagcccgta atatacgggc tttaaggatt ataaattgaa ttttattgaa aagtacatca
     7021 tcacatttaa taaatgtaat ttgtttttta ttatattatt atcaactata agtagttttc
     7081 ttttcgttct attcggatat ttaataaaaa cagttattga caataaagat tcaataggag
     7141 aaataaactc attattaata tttatagcat gtttcttatc aattagattc cttatgcctg
     7201 ctggatatag catatcggaa tacttgactc aaaaaacaaa tatagaatta tctgtaaagc
     7261 ttagagagca agttattgat aatatacaaa attctcacca agagcatttt ttaaagaaaa
     7321 ataaaggaga actaaataag gttatagaaa gcatgctttc ttctgcatca tcattatttt
     7381 atacaatatg ttctgatgta gtaccattat taatacaaat gattggaata atcataacaa
     7441 tttgtataag tgttaataca ttaatagcca ttgagtttat aataataatg gcaatctata
     7501 taatatttgt cataaaaatg acacaacgta gatttccaat gatgaaatca gttgcactta
     7561 gctccaaatt cgcatctgga agaatgtttg atatgatgca catgtatcct atggataaag
     7621 ctttccatac cacggataaa agtcgtaaac gagtaattca agctgtaaat acacattcag
     7681 aaaaacaaag aaaagtaaat aatgaatttt tcctgtttgg tatcagctcc gcttttcttt
     7741 cagtcatttt tagtagtctg attatcctat cagcatactg gatgtttctt catggtagag
     7801 caagccgtgg tagtattatt atgcttgcca cttttttatt ccaagttttt ctcccattaa
     7861 atcgcattgg ttatctattt agacaaataa aaatggcaag aacagaaatt gatttatact
     7921 gtgccgaaat aaacgacat
//
LOCUS       DAPOZO010000161         7936 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPOZO010000161 DAPOZO010000000
VERSION     DAPOZO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.162"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            1..323
                     /locus_tag="ROI21_004477"
                     /pseudo
     CDS             1..323
                     /locus_tag="ROI21_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            845..1117
                     /locus_tag="ROI21_004478"
     CDS             845..1117
                     /locus_tag="ROI21_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HDX5113312.1"
                     /translation="MTPSPYPRNNFNTELIQSCMNGEHFSLLIEISPIRSKKNIMALK
                     EYLVDGYSKQESCERNNVSISYFCLCLKKLNYTHSIVSALKYFYRE"
     gene            1563..1738
                     /locus_tag="ROI21_004479"
                     /pseudo
     CDS             1563..1738
                     /locus_tag="ROI21_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1964..3133)
                     /locus_tag="ROI21_004480"
     CDS             complement(1964..3133)
                     /locus_tag="ROI21_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113313.1"
                     /translation="MNQKWKTLIVSVLTPSSIISGIALIVLWGYFSRLDRLDIFFDVM
                     NIKSILVLVCCTTILSLIMIIFIFFITSFFIPIVIPYDIKNLPAYNKIQGNFLSVMML
                     SGLFPMAFIYVLYCAFDFDQSVKDNSGWLSILSIGVLIVVISAIINTRYLEYDLSFKN
                     NRMKLLRRVQIYLIIPLSIALLVHLQVIPLEMVFRNIETSDKSINFKVIAELAFMSYF
                     IFILTILPGLIYLKMNPQHKLSKRISSAFVASLMILLVISTQITVLPVIFTHSVIKLS
                     GISDFKIHSYIIKTSEYPEEFFSNAVWGKKNIKPGEYYSVQAVSMFTTNQFILLCPKD
                     IIRFYRESWKFDLLNVDFDTNTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPSN"
     gene            3500..3679
                     /locus_tag="ROI21_004481"
     CDS             3500..3679
                     /locus_tag="ROI21_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDX5113314.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPKNGKPALSPRHDWLNTG
                     SLLQNIPLRIWFYPQ"
     gene            3800..4540
                     /locus_tag="ROI21_004482"
     CDS             3800..4540
                     /locus_tag="ROI21_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDX5113315.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(4825..5802)
                     /locus_tag="ROI21_004483"
     CDS             complement(4825..5802)
                     /locus_tag="ROI21_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDX5113316.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            6588..7319
                     /locus_tag="ROI21_004484"
     CDS             6588..7319
                     /locus_tag="ROI21_004484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113317.1"
                     /translation="MHRLDEVVDTLLVLQKKHRIRFDVWQVVKRDHAIISFFDQGMNP
                     AVPRVAYWTPFRYPLLLNLASLFDNELAEKAWCARLEAHDGRSSSLFSEVCSELLARV
                     HTLGDRRYIELITDALSWAMTHFDELGYNCKTGKQKLQIMPNMVGFQFVLRGICSRLG
                     APNRKADIVVDQQSQFNTTQRELREFYYQIREMPWVHGPGLPVMDVTNMPAERWFSSR
                     VQKVQGWSWLIYISGSSNALWKGRS"
     gene            complement(7688..>7936)
                     /locus_tag="ROI21_004485"
     CDS             complement(7688..>7936)
                     /locus_tag="ROI21_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDX5113318.1"
                     /translation="TNDNVPGLLSLITAHLKDLPDDGRNEDVFKMLRSSAAILHGINN
                     LRNNYSMAHPTETLLNEADARFAINLVRSIMTYVDELL"
BASE COUNT         2235 a         1746 c         1791 g         2164 t
ORIGIN      
        1 ggtgtggcag ccagccagtt atttctctgg cgcaagcaat accaggaggg aagtcttact
       61 gctgtggccg caggagaaca ggtcgttcct gcctctgaac ttgctgctgc catgaagcag
      121 attaaagaac tccagcgcct gctcggcaaa aaaacgatgg aaaatgaact ccttaaagaa
      181 gccgttgaat atgggcgtgc aaaaaagtgg atagcgcacg cgcccttatt gcccggggat
      241 ggggagtaag cttagtcagc cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc
      301 tcagacgaac cgatgactgg aaatataggg gcaaatccac atccttactt ttccgtaagg
      361 attttagcgg gtcaaaacag cgttccatgc cggactgatg atctacatac ttagagtcac
      421 ataccaaagt aaaactatgg atgtatgcaa gcctgcatcg tcaggaggtt aaatttcaga
      481 cctgccagtt accgcgtaaa tggcttgata tatgactaat ttatttgcac aggattttta
      541 cgacaacgct ctgaaccagc agttgacggg atatatagtt gagctcatta cagataaagc
      601 ttttctgtat ctctggcggt ggtcatagac cagtaatcgt tattggttgt tctatattat
      661 cccgtatgac ggtacaacgt ttttaacaac tggcgctgtc acaacataag ccaccccaaa
      721 atattatatg agtaacaata gtctatatag gagcgtaaat tacctactat caaatactta
      781 ctccaatagc atttctgtct attctatttc aattctttaa tagaaaaata aggttagata
      841 atatatgact ccctccccct atccaagaaa taactttaat acagagctaa ttcaatcatg
      901 tatgaatgga gaacactttt cacttttaat tgaaatatca ccaatcagaa gtaaaaaaaa
      961 catcatggct cttaaggagt atttggttga tggttattca aagcaagaaa gttgtgaaag
     1021 gaacaatgtt tccattagtt atttttgcct atgcctaaaa aaactaaact atacacacag
     1081 tattgttagt gctcttaaat atttctaccg tgagtaaact gtaactgaag tcttgattct
     1141 tacgcttaga cactgctaag ccgttatggc atagctgata atttatattg cactatgtca
     1201 gctactgtaa gataatataa gctattttaa agaaaactct gtcattcatc attttgttta
     1261 atgataggtt ttgttattaa tataaaagaa catgctcatt cattaaaata aaaacaagat
     1321 gttcttttat tattttcatt ccctacgggg ttagtccttg attcattttc agtatcttct
     1381 aactcattca tttgccgatt actttaattc acagtcttgt tttttatcct gcctattgga
     1441 gataaaagtg gtatcgatgg atgccgagtg tgttgcacat gcagtattct caggaataag
     1501 aactgtcact tccaaaacac gcgatatatg attgaatctc tgtgggggga taaaaacttg
     1561 cctttttccg cagtctgaaa acaggatggg ggcgacggat ggttacgcag gcaagggcga
     1621 ggttcggaaa taaatcagag attatattct gaattattac aatagcacca gacctcacca
     1681 ttacaacggt agtttgacgc tggagggatc agaaaacaga taccgttttt actgtaaacg
     1741 gtggtcatat atggactctc ctggtgatat aagtactggt tcgaaacggt ttacgactat
     1801 atatccggcg tcatcagtag gttcaatgaa ccgcgcaata atgttatccg agccctgttt
     1861 cctaatcact acgccggtat ttgattagcc aagtcaagtt catcttgcca tcactatttt
     1921 tccataccgc attactatct aatacagtcg cacgagcggc cttctaattt gagggtttcg
     1981 agccctgaag aggcatatcc catcgtttta cagatattgc agaaattggt acgcaatatg
     2041 ctgcttcttc ctggagtttc ttcctggtat ttgtatcgaa gtctacatta agcaggtcga
     2101 acttccagct ttcccgataa aaccttataa tatccttggg acaaaggaga ataaactggt
     2161 ttgtcgtaaa catagaaaca gcctgcacag agtaatattc ccccggtttt atattttttt
     2221 taccccatac tgcattggaa aaaaactcct ccggatattc acttgtttta ataatgtagc
     2281 tatgtatttt gaagtcactt atacctgaga gcttaataac tgaatgggta aaaattactg
     2341 gtaatacggt tatttgagtc gatataacta ataatatcat caaggatgcg acaaatgcag
     2401 aactaattct tttagaaagt ttatgctgtg gattcatttt tagataaatg aggccgggaa
     2461 gtattgtcag aataaaaata aaatatgaca tgaaagccag ttcagctatc actttgaaat
     2521 taatactctt atctgaagtt tcaatattcc taaaaaccat ctccagtgga attacctgaa
     2581 gatgcaccag taaagcaata gacaacggaa tgataagata gatctgaaca cgtctcaaca
     2641 gtttcattct attattttta aacgataagt catattcaag atacctcgtg tttattatgg
     2701 ctgaaataac aactattagt actcctatac ttaaaataga taaccagcca gagttatctt
     2761 taacactctg gtcaaaatca aatgcacagt ataacacgta tataaaggcc atggggaaga
     2821 ggccagataa catcattaca gataaaaaat taccctgaat cttattgtaa gcaggaagat
     2881 tttttatatc ataaggaata actattggta taaaaaaaga tgttataaaa aaaataaaaa
     2941 tgatcataat caatgataga atagttgtac aacaaaccaa taccaagatg cttttaatat
     3001 tcatcacatc aaagaaaata tcaagtcgat ccagacgact gaaatatccc cagagaacta
     3061 tcaacgcaat ccctgatata atacttgatg gtgttaatac tgaaactatt agcgtcttcc
     3121 acttctgatt cattttaact tcctggtttt gctataaaga gagcataaaa aagagtgaat
     3181 aagttattac acttcattgt ttgactcctc gcagtaacct ttcaggaagg ttgtcggata
     3241 tttttgataa attatgtcat aaacatatta ttgtaagtaa tgatttcact aaatttattt
     3301 gatcgtcgtc ataatatttt taatctgaat atagaagatg attgtgtcat atgcagtatg
     3361 atataaaacg ttaaaagtga ctgaatagat aacagttcat gcatgaacaa gaccataaca
     3421 taacataata aaatatgctt attatgcata accttatgtt gtgactaagc cgccttaaaa
     3481 ctgagaagta gctaagaata tggcgtcaga caccttcagt tgtcgtagaa ggttatctgc
     3541 aggatttttc tccgtatctg cctgttcctg gcattacccc aaaaacggca aaccagccct
     3601 cagcccgcgc cacgactggc ttaatacggg atccctgctg caaaacatac ctttacgtat
     3661 ttggttttac ccgcaataat gcgtgataag caaacaaaag gatccgcgcg ctgcgattta
     3721 ttgtcgtgga aggatccggg cgttcaggtc agaatacctg tatccataaa cagtgcgtgt
     3781 gctacgtgaa aaataactca tgaacaatgt cattcccctg cagaattcac cagaacgcgt
     3841 ctccctgtta cccattgcgc cgggggtgga ttttgcaaca gcgctctccc tgagaagaat
     3901 ggccacttcc acgggggcca caccggccta cctgctggcc ccggaagtga gtgcccttct
     3961 cttctatatg ccggatcagc gtcaccatat gctgttcgcc accctctgga ataccggaat
     4021 gcgtattggc gaagcccgga tgctgacgcc ggaatcattt gacctggatg gagtaagacc
     4081 gtttgtgcgg atcctgtccg aaaaagtgcg tgcgcgacgc ggacgcccgc caaaagatga
     4141 agtgcgcctg gttccgctga cagatataag ctatgtcagg cagatggaaa gctggatgat
     4201 caccacccgg ccccgtcgtc gtgaaccatt atgggccgtg accgacgaaa ccatgcgcaa
     4261 ctggctgaag caggctgtca gacgggccga agctgacggg gtacactttt cgattccggt
     4321 cacaccacac accttccggc acagctatat catgcacatg ctctatcacc gccagccccg
     4381 gaaagtcatc caggcactgg ctggtcacag ggatccacgt tcgatggagg tttataccag
     4441 agtgtttgca ctggatatgg ctgccacgct ggcagtgcct ttcacaggtg acggtcggga
     4501 tgctgcggag atcctgcgta cactgcctcc cctgaagtga cggcatacgc gcttccttat
     4561 ataagctgtg gtcagcagaa cagacataac ataagctgga gcaggtagat aagctgtagt
     4621 gagtaaacca tgttttatga agggagcaat gcctcagcat caggttacgg ggtgactcac
     4681 gtaagggaca ggcagatggc agctcagcca caggcagcac tgcaggaaac tgaatataaa
     4741 cagcagtgag cagaccactc actgcacctg ataatataag ctgtagtcag taaaggagca
     4801 ctcttcactg actacagttt atgttcagcg ggatttgaag agtttttcca ggtcatccag
     4861 tgtgatgccc agtttttcaa gcagggcaag tttctccgcc atctccggac tgactttttc
     4921 cgcaggtgaa ggtggcaacg gattttcctt actctcatca ttcggcgctt ttaaccgggg
     4981 acgccggtag tgaatacaga agaattttgt ccgcccccgc tggatctccg tgtaatcaag
     5041 atatccaatc tcgcgcaact gctccattgc ccgtctgacc gtctggttct gggaaaatac
     5101 aggagacttc agattgaggc gtgcacgcag ccgcgccagc gatatcggtg ccggatcccg
     5161 gggcaggctc tctataaagg tgtagagtgc ctgggcggac tcccgtcgct tcagggcatt
     5221 aatcgcctta agctggagaa ggacttttct gtcaaactgg tacagttcaa acaggcgggg
     5281 atcagcctgt aactgaacaa tatcccgttc agtatcgtag taggctgact gtaccagatg
     5341 ggtgatgtat tcccgggtgt gcttctcatc ggtacgggaa aacgagatca cggtaccggc
     5401 aatgcgcttc agggaagggc tgatgcgctc acgcagcctg cgggatgact ggcttgaagg
     5461 tataccacac agttttgcaa actcaacaaa aggcagttca actttgtcac caatcacgtt
     5521 atggcgggca aaggaatgaa tgatccccac ccaggtcttg aaatcgttat ccatatccag
     5581 ccgggggccg gtgatctcaa ccttatcgaa tccttctgca cgggccagag aaagacgcgt
     5641 cagttcttcc gtggcatcgg tgcgtgacag tgtatttttt ttactgttct tcagtgactt
     5701 aagggtcggt acaaagacgc ccagacgcat cagcgccaca ggttgtacgg tgttgttatt
     5761 gtttggtgtc agtgtcacca gctcaccgga cgacttatcc acctcgccga acagtttttt
     5821 gatgtctgaa ttttcgtttt ccagaaagcc tccagcctgt ggacagtaaa agaataaaca
     5881 gaaaatcgtg tgtcagaact ccgctaactg actgaagaaa aacctgcagc ctgtatttaa
     5941 caactgagtc tggcatactg accatagttt atgtcattaa cttacttcag tatatggatt
     6001 actgaataca gcttatcatt agcatgttac agtttatcag taacatgcca cagcttatgt
     6061 gatcgtagtc ccggcccatg agtgacaagg tttgcagaga tatggggatc tttaatggat
     6121 cctttgttga tcattcatgg atctgtttac tggatcgggc ctccggatat gtggataaat
     6181 cattccagaa aaacagcata cactaccgat tatgaaggct tcagggcgta cctgctcact
     6241 gtaactacag atccattgcg cttcctcgcc ggttcgcggg cttcgcccgg tcaccggtct
     6301 gcggcgagca ctgtttttct gatgtctgtc tttacaggta atttttgacg gaatctgccg
     6361 tacttcctga gcgtacggtc atatccgaag tacacttcat atgtgtgttt caatgccagg
     6421 gattgtgtat acaaactaca ggcaaagtct ggttatcacc ggtggtaggc tgctccgatg
     6481 gaaaaattgt gaacggattg acaccatgcc ggataactct gtcgatataa gacggtcaaa
     6541 gatcgtggcg ttgacaagta cagcgtctgc acgccgcaga gcttggcatg caccgccttg
     6601 atgaggtggt tgatacgctg cttgtcctgc agaagaagca ccggatacgg tttgatgtct
     6661 ggcaggtcgt taaacgggat cacgccatta tttcgttctt cgatcagggg atgaatcctg
     6721 cagtgcccag ggttgcctac tggaccccct ttcgttaccc gctgctactg aatctggcaa
     6781 gcctgttcga taatgaactg gctgaaaaag cgtggtgtgc ccgcctcgaa gcccatgatg
     6841 gaaggtcctc atctctgttc agtgaggtct gcagtgagct gcttgcgcgc gttcataccc
     6901 tgggggaccg ccgttacatt gaattgatta ccgatgcgtt gtcctgggcg atgacccact
     6961 ttgacgaact gggatataac tgcaaaaccg gtaaacaaaa gctgcagatc atgcccaata
     7021 tggttggatt tcagttcgta ttgcgcggta tctgttcccg cctgggagca ccaaatcgca
     7081 aagccgacat cgttgtggac cagcagtcac agttcaacac cacccagcgg gagctgaggg
     7141 agttctatta ccagatacgt gagatgccgt gggtacacgg tcccggttta ccggtcatgg
     7201 atgtgacgaa catgccggct gaacgctggt tttccagtcg ggtacaaaaa gtgcagggct
     7261 ggagctggtt gatatatatc tctggatctt caaacgcttt atggaaggga aggagttaac
     7321 cagaccccct tacccgcctg gtttacacca atcgcaatac cggcaggacg gacagtgtct
     7381 cccttcagtc ggtggctaaa cgatctaagg agtttctcga caaactgcag gagccaacgg
     7441 cagaaatgat gaaaaaggcg agggaatacc gggatcagga agaggcaagg cggctggaac
     7501 atcgggtgca aatcctgccg ccatcctgat ccggcaggtt gagtacacac aaaaatggaa
     7561 gcggccacga cgggatgatg cgtctgtccg gcatcttccg gagggccaga actcgaaatt
     7621 ctggccgtac ttcacttctt cgttgaagtt ctatctgttc gctgtgagtc tcatattttt
     7681 attaaaacta aagcaactca tcaacataag tcatgatgga acgaaccaga tttatggcaa
     7741 aacgggcatc ggcttcattc agcaaagttt cagtaggatg ggccatgctg taattgttac
     7801 gcaggttatt gatcccatga agtattgcgg cagatgatct caacatttta aacacatctt
     7861 catttcttcc atcatcaggc aggtctttga gatgtgcagt tatcagggaa agcaagcctg
     7921 gaacattatc atttgt
//
LOCUS       DAPOZO010000162         7878 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPOZO010000162 DAPOZO010000000
VERSION     DAPOZO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.163"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(897..1103)
                     /locus_tag="ROI21_004486"
     CDS             complement(897..1103)
                     /locus_tag="ROI21_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HDX5113319.1"
                     /translation="MFLIYITLPFIQLVYFISSEKKLTIHIVQMFQLLSQAFYNLKMF
                     LMMDMLGVGDAININTNKNIRQVC"
     gene            complement(1184..1801)
                     /locus_tag="ROI21_004487"
     CDS             complement(1184..1801)
                     /locus_tag="ROI21_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDX5113320.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKNNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFHDNAQNISAIAEAVCQLPKLQEVLKRNEII"
     gene            2068..3567
                     /gene="ansP"
                     /locus_tag="ROI21_004488"
     CDS             2068..3567
                     /gene="ansP"
                     /locus_tag="ROI21_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDX5113321.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEQQEIVFKPETAS"
     gene            complement(3680..4741)
                     /locus_tag="ROI21_004489"
     CDS             complement(3680..4741)
                     /locus_tag="ROI21_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDX5113322.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILSKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            4983..7085
                     /locus_tag="ROI21_004490"
     CDS             4983..7085
                     /locus_tag="ROI21_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDX5113323.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSATPQVVSELDTP
                     AAVSVVNGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGAMGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPLTFTTGLNYENMSENRKGYNNFRLNSGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAEMAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            complement(7121..7786)
                     /gene="mcbR"
                     /locus_tag="ROI21_004491"
     CDS             complement(7121..7786)
                     /gene="mcbR"
                     /locus_tag="ROI21_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDX5113324.1"
                     /translation="MPGTEKIPHISLTMKVENDLKHQLSIGALKPGVRLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPQDLAELEELLEKLQQAQEKGDMEQIVNANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT         1998 a         1845 c         1888 g         2147 t
ORIGIN      
        1 tgactggttc agatgaaagg agaccaccgc aaacgcgtcc ggtggcaggc cgtccacttc
       61 cagcgtgaaa cgtaatccgg ctgacatagc aacctccgtg aaaatgaata ataatccgca
      121 catattgcat gtacaacatg tcgggcggat gagataagcg agtcgacatc tggcgttacc
      181 ggggaatatc aaaaaaatgt catggttatg tgatgattta gtctgggatt ttacttcaga
      241 gaaacgggtg tctgtttagt caacaaaata atcacaaacg caacggatgg gttctcgacg
      301 ttattcacga caggctcgcg agtaataaaa acctgatatg caaagtcaaa ccaatattca
      361 taatgtcttt ttattcacaa caataaataa ttcaataaca atcattgaaa tgaatgatgg
      421 tttgtataag gaatatacgc taccgcccca ccctgaaaac atgaactgca cattgccatc
      481 ttaacgtcgt taaacactgg cttaatagtg ggtatttaaa attaggaatt tattaaattc
      541 acctgatgaa tatcataagc aaatagcata gaaaataatg acaactcccc cgaggggacc
      601 atttccaaag cactgcacca ccacctggtg cccggatgat cttgcttcat tggtaagagt
      661 acctgattca ttgtttccaa aatggtgttt tccccggccc ctccaataat gtgcagctct
      721 cacgtgataa tctattgatt aggttatatt aagaatcaat atttgaaata catccatttt
      781 cttgaataaa tcaacatgtt tatttatcaa ttgctgtttt agctctgact catcaagctc
      841 tccatatttg tcctacagat aatcgcgaat aaatacagag aacgccattt atccttttag
      901 catacctgcc ggatattttt atttgtatta atattaattg catctccaac tccgagcata
      961 tccatcatta aaaacatttt gagattatag aatgcctgtg ataacaactg aaacatctgc
     1021 acaatgtgaa tagttagttt cttttcagag gaaatgaaat agacaagctg aatgaaaggg
     1081 agagtaatat aaattaaaaa aataacatga atctaattaa ttacgtccat cattgtggat
     1141 aaagttactg aataataaat aacataatct tctggcatcc cactcaaata atttcattcc
     1201 tttttaatac ttcttgtaat ttcggtaact gacatacagc ttcagcaatc gcactgatat
     1261 tctgcgcatt atcgtgaaac cattcatgcc ccggccccca tgtgcgcatg gtgcagagat
     1321 aacagtccac cagcgttagt tgctcgccaa aggcataagg ttcagcggtc aattgtgaat
     1381 tcagccagat ataaagcgat ttacgatatt caataacatt attctttaac tgttcgggtg
     1441 cgtcaggagc ccaacgctca gggtaatcgg cgaaagtgaa tgttggatag acattagcaa
     1501 ccagccagac caatagccgt tgaaactgct gacgttcggc gcgcccaacc ggtggcgcaa
     1561 ggtccgggcg acgatcgaga accatcaatg caatcgccgc tgtctccgtc ataatctcgt
     1621 cattttccag cgccagtgtc ggaatctgac acaacgggtt cagggttttc aataactcgc
     1681 gtgaggttcc ctcgtggtca aaaccactga catcaacgaa ctgataagga atatcagcca
     1741 gtgttagcat cagctcactg attgtagagc cccatccggg tacgccatac accttaatca
     1801 tatgcccctc acaatctttt aaagattaag tgtaggacag tacacaggtt aaccagcaaa
     1861 aggatttgtc attttttcgt cagttgtatt attccccttc aggattgtaa ggggatgttt
     1921 tgcatcgttt atcgataacg ggtaggatgc tgcgccgata gttttatacc cataaagaat
     1981 aatggtgata actatcatcg ccaggatgaa taaacattgt tcatggcaac ttatatgact
     2041 ttttcattaa agcaatcagg gagagcaatg agtaaacacg acaccgacac ttcagatcaa
     2101 cacgccgcga aacgccgctg gcttaatgcc cacgaagagg ggtatcacaa agcgatgggc
     2161 aatcgccagg tacagatgat cgccattggc ggcgcgattg gtacgggttt gtttttaggc
     2221 gcgggagccc gactgcaaat ggcgggtcca gcactggcac tggtctattt aatttgtggc
     2281 ttgttttcgt tttttattct gcgtgcattg ggtgagctgg tgttacaccg cccttccagt
     2341 ggcagttttg tctcttatgc ccgtgagttt ttaggagaga aagccgctta cgttgcaggc
     2401 tggatgtact tcatcaactg ggcgatgacg gggattgtcg atattaccgc cgttgcactg
     2461 tatatgcatt actggggcgc atttggtggt gtaccgcagt gggtctttgc gctcgctgca
     2521 cttaccatcg ttggcaccat gaatatgatc ggtgtgaaat ggtttgcgga gatggagttc
     2581 tggtttgcgc ttattaaagt gctcgccatt gtgacctttt tggtcgtggg tacagtgttc
     2641 ctcggtagtg gtcagccgct ggatggcaac accactggct ttcatttaat caccgataat
     2701 ggcggcttct tcccccacgg tttgctgcct gcgctggtgt tgattcaggg cgtagtgttt
     2761 gcttttgcct ccattgaaat ggtgggtaca gctgccggag aatgtaaaga tccgcagacc
     2821 atggtgccta aagcgattaa cagtgtgatt tggcgtattg gcctgtttta cgtcggctcc
     2881 gtggtgttgc tggttatgtt attgccgtgg agcgcgtatc aggcggggca aagtccgttc
     2941 gtgacgtttt tctctaaact gggtgtgcca tatatcggca gcattatgaa cattgtggtg
     3001 ctgaccgctg ccctctccag cctgaattca ggtctgtact gcaccggacg tattctgcgc
     3061 tcaatggcga tgggcggttc cgcaccgagt tttatggcga aaatgagtcg tcagcatgtg
     3121 ccgtatgccg ggattctggc gacactggtt gtgtatgtcg tcggcgtatt cctcaactat
     3181 ctggtgccgt cgcgcgtatt tgagattgtg ttgaacttcg cgtcgctggg aatcatcgct
     3241 tcatgggcgt ttatcatcgt gtgccagatg cgcctgcgta aagcgattaa agaaggcaaa
     3301 gccgcggatg tcagttttaa actgcctggc gcgcccttca cttcctggct gacattactg
     3361 tttttgctga gcgtcctggt gctgatggcg ttcgattacc cgaacgggac ttataccatc
     3421 gcggcgctgc cgattatcgg tattctgctg gttataggct ggtttggtgt gcgcaaacgc
     3481 gttgctgaaa ttcacagcac tgcgccagtc gtcgaggaag atgaagaaca acaggaaatt
     3541 gtgtttaagc ctgaaacggc gagttaattg caaaattgcc cggctacgtg ccgggcaaat
     3601 tcaaattact gacgaaggtg ccggatacgg cgtgataaaa gtcaggcaag tctctccgtt
     3661 ttcaccctcg ccatatctat tacagcgcaa tacgaatcac atcgtccggc tgggtagctt
     3721 cctgctgttt agtggatttt tgtttcacac tgacatacag cgttttgcca tcggcagaca
     3781 gcgccaggct gttcggatga gtcggcgtat cgaacgtttt caccacttta tagcttttcg
     3841 cgtcaatcac actgacttta cctgcctgac gatgcgttac gtaggcttca ttacgcgccg
     3901 ggttaaacag cacagccagt gattccggtg ccgcaacctt cgacagaata ttgccattac
     3961 gggtatcgac cactaacact tctgcggctt tagaatcggt gataaatgca cgctgcctgg
     4021 cggtatcaag gctaatgttg ataaagaagt gctctttgcc gtcatccagc agctttttac
     4081 ggctgaggat tttattgtcg gcggtgtcga tggtaatcaa ttcgccgtca gcgttagtgg
     4141 tgtaaagacg tttgcctttg ctatccagcg ccagaccggt actcatttta ccggtgttct
     4201 ggatggcggt tttcagtttg atattctcgc catcaacgac ccaaatcacg ctctctttac
     4261 caataccact gatgtaaacg gtgttcgtgg catcatcagc taccagctca cgcggttgca
     4321 gcgggcgcac ctcttccgtg cgcttacgat catccagcac cagacggcct ttcacttcgc
     4381 ccgttttggc atctatcgcc gtgaccgcgc tgtttacagt gttaccaaac cacaacgtct
     4441 gagtcgtgtt attgatggta gcaccaaacg gcttgagatc gttatggatc gcctgcgtca
     4501 cttccagagt aaccggatca agacgataaa ccacgccgcc tttatccagt ttgcggcttt
     4561 gcgaagtggc gagccacagc gcgttttctt gctggctata agccatttcg taggcacctt
     4621 tacctaccgc tttacgcagc atttcttctg cggcctgcgt actgaatgat gaagcaacaa
     4681 gcaatgaacc taacagtaat gaaccacgca ggcgcgatga aaacagatga cgtaaatgca
     4741 tgacgactcc ctttgatgaa ccgatatttt tgctgcttca caatgcttcg ctaagcgtaa
     4801 tcatcgttac gctcttgtta taaatgggaa tggtaatcat tattttctca aatgtggagc
     4861 atttcttgcc gttacgacta caattttctc aattacttaa cgtatcgtgc cgtttcgttt
     4921 tccataaatt tacaatttta ttaccatagc ggcgttttcc cattcttaac ctggcttttt
     4981 tcatgaagat tttttccgtc cgacagaccg ttttgcccgc actgcttgtc ctttcccctg
     5041 ttgtttttgc cgctgatgaa cagaccatga ttgtcagtgc cacgccgcag gtggtttcag
     5101 aactggatac cccagcagcc gtgagcgtgg tgaatggcga ggagatgcgt ctggcaacac
     5161 cgcgcattaa cttgtctgaa tcactgaccg gcgtgcctgg tttgcaggta caaaaccggc
     5221 agaactatgc gcaagattta cagctgtcga ttcgcggatt tggctcccgc tccacttacg
     5281 gaattcgtgg tattcgcctg tatgtggacg gtattcccgc caccatgccc gacggacaag
     5341 ggcaaacatc caacatcgat ttaagcagtg tacaaaatgt ggaagtgctg cgcggcccct
     5401 tctctgctct gtatggcaac gcgtctggcg gtgtaatgaa tgtcatcacc cagaccggac
     5461 aacagccacc aaccattgaa gccagtagtt actacggcag ttttggcagc tggcgctatg
     5521 ggctgaaagc aacaggcgca atgggcgacg gcactcagcc tggcgatgtc gattacaccg
     5581 tctcaacaac gcgctttacg acccacggct atcgtgatca tagtggcgca cagaaaaatt
     5641 tagccaatgc caaactgggc gtacgcattg atgacgccag caaattaagc ctgattttca
     5701 atagtgtgga tatcaaagca gatgacccag gtgggctaac cgaagcagaa tggaaggcga
     5761 atccgcaaca agcgccacgc gctgaacagt acgacacgcg aaaaaccatc aagcaaactc
     5821 aggctgggtt gcgctatgaa cgtagcctga gcgcacaaga tgatatgagt gtgatgatgt
     5881 atgccgggga gcgagaaacg acccagtacc agtcaatccc gatggcacca caacttaacc
     5941 cgtcgcatgc gggcggcgtg attactctgc aacgccatta tcaggggata gacagccgct
     6001 ggacacaccg tggcgagctg ggcgttccgc tcacgttcac taccggcctg aactacgaaa
     6061 acatgagtga aaatcgcaaa ggctacaata acttccgcct gaatagcggc gtgccggaat
     6121 acgggcaaaa aggtgagttg cgccgcgacg aacgcaatct gatgtggaac gtcgatccct
     6181 atttacagac gcagtggcag ctgagcgaaa aactgtcgct ggatgctggc gtgcgctaca
     6241 gctccgtgtg gtttgattcc aacgaccatt acgttactcc gggtaacggc gatgacagcg
     6301 gtgatgccag ttatcacaaa tggctacctg ccggatcgtt aaaatatgca atgaccgacg
     6361 cctggaatat ctatctggca gccgggcgtg gttttgaaac gccgacgatt aatgagctct
     6421 cttatcgcgc tgatgggcaa agcggtatga actttggttt aaaaccatcc accaacgata
     6481 caattgaaat cggcagtaaa acgcgtattg gtgatgggct gcttagtctc gcattgtttc
     6541 agaccgacac tgatgatgaa attgttgtcg atagcagtag cggtgggcgt acgacctaca
     6601 aaaatgccgg aaagacccgt cgtcaaggag ctgaaatggc atgggatcaa cgtttcgcgg
     6661 gagattttcg cgtaaaagcg tcctggacct ggcttgatgc gacctatcgc agcaatgtgt
     6721 gcaatgaaca ggattgtaac ggtaaccgga tgccagggat cgcccgtaat atgggctttg
     6781 cgtcgatagg ttatgtaccg gaagatggct ggtatgcagg cacggaagcg cgttatatgg
     6841 gtgatattat ggcagatgat gaaaatacgg ccaaagcgcc gtcttatact ctcgtcggct
     6901 tattcaccgg gtataaatac aattaccaca atttaactgt ggatttattt ggtcgtgtcg
     6961 ataatttatt cgataaagaa tacgttggtt ctgtcattgt caatgagtca aacgggcgat
     7021 attacgaacc tgcacccggg cgaaattatg gtgtcggcat gaatattgcg tggcgatttg
     7081 agtaattacc ttgatgcccg gtatttgccg ggcatttaat ttaacgattg tattgctggt
     7141 ataaaatagc aatattttgt tgcataattt cagcaagaca atgcctgcat ccctctttgt
     7201 cttttgcttt taacgcggcg agtaatagac gatagttttc tatacgctcc cgtaattccg
     7261 ctggattaat cgcttcatag agataatgta aaccaggtcc catcctgacc cacagttgct
     7321 caatcatctc acacaggatg ggcatatttg aacgatgata aatcgctaag cgaaatagcc
     7381 tgttcgcatt aacgatttgt tccatgtcgc ccttttcttg cgcctgctgt aatttctcga
     7441 gcaattcctc gagttccgcc aggtcttgcg gggtgaggtt ttcaacagcc agagtaactg
     7501 ccattaattc cagctcgtag cggatccgat tgatttcgtc taattgacgt ttccccactt
     7561 ccggaactgt aaatgcttgc gctggtgcga cagaaagcgc attcaccgaa accagacgta
     7621 ataatgcttc acgcacgggt gtaatactca tacctaattg ctccgccaga tttttagtaa
     7681 tcaggcgtac gccaggtttt agtgcgccaa tactaagctg atgtttcagg tcgttctcaa
     7741 ccttcatagt taaactgata tgcggtattt tttccgttcc aggcatgtta tttcctttag
     7801 gcgttttcgt tccaactctg ccagcatagc agaagtcgtt tacctggcgt atgaagctga
     7861 ataatcatta tccactca
//
LOCUS       DAPOZO010000163         7789 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPOZO010000163 DAPOZO010000000
VERSION     DAPOZO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.164"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(99..251)
                     /locus_tag="ROI21_004492"
     CDS             complement(99..251)
                     /locus_tag="ROI21_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDX5113325.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(516..1250)
                     /gene="cysH"
                     /locus_tag="ROI21_004493"
     CDS             complement(516..1250)
                     /gene="cysH"
                     /locus_tag="ROI21_004493"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDX5113326.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRTNLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1324..3036)
                     /gene="cysI"
                     /locus_tag="ROI21_004494"
     CDS             complement(1324..3036)
                     /gene="cysI"
                     /locus_tag="ROI21_004494"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDX5113327.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3036..4835)
                     /gene="cysJ"
                     /locus_tag="ROI21_004495"
     CDS             complement(3036..4835)
                     /gene="cysJ"
                     /locus_tag="ROI21_004495"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDX5113328.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVNVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5154..5516
                     /gene="queD"
                     /locus_tag="ROI21_004496"
     CDS             5154..5516
                     /gene="queD"
                     /locus_tag="ROI21_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDX5113329.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5594..6865
                     /locus_tag="ROI21_004497"
     CDS             5594..6865
                     /locus_tag="ROI21_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDX5113330.1"
                     /translation="MEDNCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6856..7116
                     /locus_tag="ROI21_004498"
     CDS             6856..7116
                     /locus_tag="ROI21_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDX5113331.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7133..7708
                     /locus_tag="ROI21_004499"
     CDS             7133..7708
                     /locus_tag="ROI21_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDX5113332.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
BASE COUNT         1718 a         2090 c         2046 g         1935 t
ORIGIN      
        1 ataggcataa tattcacata aacaatataa attaaaaaag ggtgcaggag accaaaccat
       61 cacaattcaa tacgcggggt tttacccccg cgtggtggct acttcttcga ttcgtaagcg
      121 agaactgcct taaactccat accacgttct ctgatactca actcacacaa cgagtcgcct
      181 accatcagcg tgaatcccag tactgtacaa cacagtacga tgattgccac aagggcatat
      241 tttgtcagca tgtcttgcct ccctgcaaag aagagactaa cattcgaatt gcttaggttc
      301 gaacgctggc ctcaggttga tagaaatatc gcctggggct tttgtccatc tggaacctcg
      361 cgaatgctta acgccagaca gcctcaagca cccaacgcca ttctatacct gataattctt
      421 ccagcggcgt tcacgtttaa aatcaggaat gctgctcgca aaataaaaac gcccccatca
      481 tttctgacag aggcgtttaa tttatccggc aatatttacc cttcgtgtaa cccacattct
      541 ctttttaagc caaagaaacg cgtttcttct tccgccatgc cgggttccca tttacgggtt
      601 gtatgggtat caccgaccga taaatatcct tcatcccata atgggtgata tttcaggcca
      661 tgtttttgca ggtactgata aatagttcgg ttatcccagt cgataatcgg cagcacttta
      721 aatacgccac gctgaattgc cagcaccggt aaattggtgc ggctgccgga ttgttcacgg
      781 cgcaggccag caaaccaggt ttgcgcattc agttctttca gcgcccggtt catcggttcg
      841 actttgttga tgtcattgta cttttcaatg ccttcaacac cctgctccca cagttttccg
      901 taacgtgctt cctgccaggc ggcgctttcg gtagcgcggt acactttcag gttgagcttg
      961 agtttgtccg ttaactcgtc aataaagcgg taggtttccg ggaacaagta acccgtatcg
     1021 gtgaggatca ctggaatatc cgggcgaatc tgattcacca gatgcaggct caccgccgcc
     1081 tgaatgccga aactggaaga aagtacatat tcaccgggca gattatccag cgcccaggca
     1141 acgcggcctt cagcgtccag tttttccagt tcggcgttag tttctgccag cgccagaatg
     1201 cgatctacct tcggcagttc gttcagggcg tttagatcga gtttggacat agattcctca
     1261 ctgtttgcct tgcctgatgc gacgctcacg cgtcttatca ggcctacaag accgggctga
     1321 tggttaatcc cacaaatcgc gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac
     1381 cgtaaagtcg ccgaagcctt cacccgcttc gcgctctttc gcccagcgcc ctatcagttc
     1441 atcaagcgac gccaggattt ccggttcggt gatgttttct ttatacatcc gtgggatacg
     1501 tgtcccaatg cggttgccgc caagatgcag gttgtagcga cccggcgctt tacccaccag
     1561 gcccacttcc gccagcatcg cgcgaccaca accgttcggg cagcctgtta cacgcatcac
     1621 gatatgctca tcgctgacac catgtttcgc cattaaatta tcgatgttgt cgataaaaga
     1681 cggcaggaaa cgctcggctt cggccatcgc cagcgggcaa gtcgggaatg acacgcaggc
     1741 cattgagttt tcacgctgcg gcgtgacggc attcattaac ccgctctctt tggcaatctt
     1801 ctcgatcttc gctttctcgc tttccggcac accggcgatg atcagattct ggttcgccgt
     1861 aatgcggaaa tcgcctttgt ggatcttcgc gatctccagc aggccggttt tcagcggacg
     1921 cccaggataa tcaaggatgc gaccattttc gataaacagc gtcaggtgcc agttatcatc
     1981 aatgccttta acccagccaa tacgatcgcc gcgtccggtg aactcatatg gacggatcgg
     2041 ttcaaatttg atccccgcgc gacgctccac ttccgcttta aacgtctcaa cccccacgcg
     2101 ctccagcgtg tatttggttt tggcattttt acgatcggta cggttacccc agtcgcgctg
     2161 agtcgtcacg acggcttctg ccaccgccag cgtatgctcc agcggcagat agccaaactc
     2221 actcgccgtg cgggcgtagg ttttcttgtt gccgtgttcg atagaaagcc caccgcccac
     2281 caacaggtta aagcccacca gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt
     2341 ggcatgcaga tcgatatcgt tctgcggcgg gatcactacc gtagttttga atttacgcgg
     2401 caaataagtc tgaccgagga tcggttcttc atcggtggtg gcgacttttt cctgatcgag
     2461 ccagatctcc gcatacgcgc gggtacgcgg cagcagatgt tcagagattt tcttcgccca
     2521 ttcatacgct tccgcgtgta gctgcgactc gtaagggttc gaggtgcaga gtacgttacg
     2581 gttcatgtcg ttagcagtcg ccagcgcatc aagaccgacc gagtgcagca tctggtgcac
     2641 cggtttgacg ttctttttca gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg
     2701 aatgctgcca tagatggtgt tttcaccggc aaatttatcg atcgcctgcc actgtttggt
     2761 ggtaatcacg ccacccggca gacggcagcg caacagcatt gcatgacgtg gctccagctt
     2821 ctgttcagca cgttcggcgc ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg
     2881 gatcagcagg aagttatcgc ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc
     2941 aatggtgccg cgcaggtagt tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc
     3001 ttcgaccact aaaggccctg gatgtttttc gctcattagt agacatctcg ctgataacgg
     3061 cgctctacgc gcagctcact taaaaattca tccgccgctt cggtgtccat gccaccaaat
     3121 tcggcaatca cttccagaag tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg
     3181 cagacataaa tgtgggcacc gtcattgatc cagcgccaca gctctgcgcc ctgttcgcgc
     3241 agtttgtctt gtacgtaaat tttttctttt tgatcgcgcg accaggcaag atcgatacgc
     3301 gtcagcacgc cctctttgac gtagcgctgc cattccacct ggtagaggaa atcttccgta
     3361 aagtgcgggt tgccaaagaa cagccagttt ttccccggcg cttcgtcggc ggcgcgttgc
     3421 tgcataaagg cgcggaacgg cgcgatgccg gtgcctgggc caatcataat caccggcgtt
     3481 tccggattgg ctggcaggcg gaagttatcg ttatgttcga taaagacgcg gacttcaccc
     3541 tcttcttcca cgcgatccgc gaggaagctg gaggcaccac cggcacgggc gcggccttcc
     3601 acgtcgtaac gcaccacgcc aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag
     3661 gaggcgatgg aatacaggcg cggggtcagc gggcgcagca ggttaatcag cgcttcggca
     3721 tccagttgcg ccggagagaa acgcaccatg tcgacaatcg gcgtcgtcgc ggcgtaatgc
     3781 tgtaacttcg ctttatcgcc caccagcggc agcagcgttt cgctgcgggt aagcgtggca
     3841 tagttctcaa caatgttggc ggtgttgacg gtcagttcga agtgccactg tagcgcttcg
     3901 ttcagcggca gggttttgcc ctcaacgttg acaggttcat cacctttcag ccacagcagt
     3961 tcgacaagtt ctttcaccag tgccggatcg ttctgatacc agacgcccag cgcgtcaccc
     4021 ggctggtaac gcaggcccga gtcacctaag tcaatttcga tatggcgaac gtctttttca
     4081 gagttacgcc cggtaatttt ctggttaaca gagaggctcg ccaccagcgg cgcgtctttg
     4141 ctgtacgggc tggtgtggat ttcatttacc gcgccagtag cgacggattg cgaaggtgcc
     4201 gcgacaggcg cacgcgattt aagcgcatca accacgcggg cgcgccactc gatggcagca
     4261 gcctggtatt caacgtcggc atcgacacgg tcgagcaggc gttcaccgcc cagttccgcc
     4321 agcttgctgt caaaatcttt cccggactgg cagaaaaatt cataagagct atcgccgagg
     4381 ctaaacacgg caaatgcggt gttttccagc ttcggcgctt ttttggagaa caggaactta
     4441 tgcagcgcga cggcttcttc cggcggttcc ccttctcctt gcgttgacgt caccacgata
     4501 agcagttttt cgctggcgat ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg
     4561 ttcagttttg ccgccaataa atcatcgcgt aatgcttcag caacccggcg cgcattgccg
     4621 gtttgcgaag cggagataat agttataccc ggcatttctg cggcgggcgc tggcgtcgct
     4681 gcaagcgcag ctggctgctg gttgagcacg ccccagaaat agccagaaac ccaggcaagc
     4741 tgggtgggag ttaaatcggt cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac
     4801 ggaagcaacg cggaaggtgg gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa
     4861 gctgtttctg cgccctgtca gcgcccataa aacagaagag aagataaggt taacggggtg
     4921 aacggtgtgg attaaagacg ggatagcgat aactaataac caaatcgact aacctgtttt
     4981 agcaatagtc tttaacaaca aaatagatta accaacccaa tgaaaaacaa atgaatattg
     5041 cccatcaata agataaatca gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa
     5101 aacagccttt ccggtactct acggcggttt tatcgtcctg tagagaaatt atgatgtcca
     5161 ccacgttatt taaagatttc accttcgaag ccgctcaccg cttaccacac gtcccggaag
     5221 ggcataaatg tggtcgcctg cacgggcatt cctttatggt gcgactggaa attaccgggg
     5281 aagtcgatcc gcatacgggc tggattatcg atttcgctga actgaaagcg gcgtttaagc
     5341 caacctacga gcgcctcgat caccattatc tcaatgatat tccaggtctg gaaaacccaa
     5401 ccagcgaagt tttagcaaaa tggatttggg atcaggttaa acccgttgtg ccgctgttaa
     5461 gtgcggtgat ggtaaaagaa acctgtactg caggctgtat ctatcgcggc gaatgataag
     5521 agtgtgtcgg cggtcaattt cccttaagta acgccatgtt agggtgttgt gttctggata
     5581 tctggggcat gacatggaag acaactgcga cattattatt attggtgccg gtatagcagg
     5641 taccgcttgt gctttacgct gcgcgcgggc aggtttgtcc gttctgttac tggaacgcgc
     5701 tgaaatcccc ggcagcaaaa atctttccgg cgggcgttta tacacccatg cgctcgcgga
     5761 actcctcccg caatttcatc tgaccgcgcc tcttgaacga cgcatcactc acgaaagcct
     5821 ttctctgcta acgccggatg gcgcaacgac gttttccagc ttacagcccg gcggtgaatc
     5881 ctggagtgta ttacgtgcac ggttcgatcc gtggctggtt gccgaagccg aaaaagaagg
     5941 tgtcgaatgc atccccggtg cgacggtgga tgcgctgtat gaagaaaacg gcagggtgtg
     6001 tggtgtcatt tgtggtgacg atattctccg cgcccgttat gtggtgctgg cagaaggtgc
     6061 caacagcgtc ctggctgaac gtcacgggtt agtgactcgt cctgctggcg aagcgatggc
     6121 gttggggatc aaagaagtgc tgtcgctgga aacatccgct attgaagaac gttttcatct
     6181 ggagaataac gaaggcgcag cgttgctgtt cagcggcggg atctgtgatg acttacccgg
     6241 cggcgcattt ctttatacta atcaacaaac gctctcgtta gggattgttt gcccgctctc
     6301 ttcccttacg caaagtcgtg ttccggcaag cgagctgctg gctcgcttta aaacgcatcc
     6361 ggcagtgcgc ccgcttatca aaaacacgga atcactggag tatggtgcgc atctggtgcc
     6421 agaaggtggc ttgcacagta tgccggtgca atacgccggt aacggctggc tgctgctggg
     6481 cgatgcgttg cgcagttgcg tcaataccgg aatttccgtg cgcggcatgg atatggcgct
     6541 aactggcgcg caggcggcgg cacaaacgct gataagcgcc tgccagcacc gcgagccgca
     6601 aaatctgttt ccgctttatc atcacaacgt cgagcgcagc ctgctatggg atgttctaca
     6661 acgttatcag catgttccgg cgcttttgca acgccctggc tggtaccgtg cgtggcctgc
     6721 gttaatgcag gatatttccc gcgatttatg ggatcagggt gataaacctg ttccaccgct
     6781 gcgccagtta ttctggcgtc atttacgtcg tcacggcctg tggcatctgg cgggcgatgt
     6841 tatcaggagt ctgcgatgtc tgtagcccgt aatctctggc gcgttgccga tgcgccgcac
     6901 attgttccgg ctgactccgt tgagcgccag acggtacaac ggttgattaa cgcttgtccg
     6961 gctggtcttt tttcgctcac accggaaggt gacttacgcg ttgattatcg cggctgcctg
     7021 gagtgtggca cctgccgttt gctgtgcgac gaatcaacac tacaacagtg gcgctatccg
     7081 ccttccggat tcggcatcac ctaccgcttt ggataaataa taaggataat ttatgcccct
     7141 cttacacctg ctccgccaga atccggtgat tgctgccgtt aaagataatg ccagcctgca
     7201 actggcaatc gattctgaat gccagtttat ttcagtgttg tacggcaata tctgcaccat
     7261 cagtaatatc gttaaaaaga ttaaaaacgc cggaaaatat gcctttattc atgttgattt
     7321 gctggaaggc gcatcgaaca aagaagtggt gattcagttt ctgaaactgg tgaccgaggc
     7381 ggacggcatt atcagcacga aagcatcgat gctgaaagcg gcaagagcgg aaggtttttt
     7441 ctgtattcat cgcctgttta ttgttgattc gatttcgttt cacaacattg ataagcaagt
     7501 tgcgcaatcg aatccggatt gtattgagat cctgccaggc tgtatgccta aagtgctggg
     7561 ctgggtgacg gagaaaatcc gccaaccgct gattgccggc gggctggtgt gcgatgaaga
     7621 agatgcgcgt aatgcgatta acgcgggtgt tgtggcgctt tccaccacga ataccggggt
     7681 ctggacgtta gcgaaaaaat tgctttgacg ggatactcgt aacgagttga atttgtgtga
     7741 tttgtaggct gcacgccaca tccgacattc agcgcctgat gcgacgctt
//
LOCUS       DAPOZO010000164         7607 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPOZO010000164 DAPOZO010000000
VERSION     DAPOZO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.165"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(157..3273)
                     /locus_tag="ROI21_004500"
     CDS             complement(157..3273)
                     /locus_tag="ROI21_004500"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HDX5113333.1"
                     /translation="MTHQTHTIAESNNFIVLDKYIKAEPTGDSYQSESDLERELIQDL
                     RNQGYEFISVKSQSAMLANVREQLQNLNGVVFNDSEWRRFTEQYLDNPSDGILDKTRK
                     IHIDYICDFIFDDERLENIYLIDKKNLMRNKVQIIQQFEQTGSHANRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDSSQTLLVMRPYQ
                     IAATERILWKINSSYKAKNWSTPESGGFIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFKRYYQFGFTGTPIFPENALGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAAN
                     KSATYKPLRIATIFSFAANEEQNAIGEISDETFDTSAMDSSAKEFLDAAIREYNSHFK
                     TNFSTDSNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVAELEQRFPDPASIESEKEKKDFVKLFGEYLRTENILQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKLAELQTIRLPADRKIQDYRSAYNDIRDW
                     QRREKEAEKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHNRQNKGKGEMIEEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIDAFFTFAQHEQLREAEALIKEE
                     NLNEEAAKRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQAVFQKIVSFIEK
                     FKGVGGKI"
     gene            complement(3395..4678)
                     /locus_tag="ROI21_004501"
     CDS             complement(3395..4678)
                     /locus_tag="ROI21_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDX5113334.1"
                     /translation="MSELSYLEKLLDGVEVEWKTLEDISIKISSGGTPKTGVSEFYDG
                     DIPWLRTQEVNFCDIWDTEVKITESGVKNSSAKWIPKNCVIVAMYGATVGKIGINKIP
                     MTTNQACANIQLNEEVAHYRYVFHFLCSQYTYIKSLGTGSQTNINAQIVKNIKIPIPC
                     PDNPEKSLAIQSEIVRILDKFTALTAELTAELTAELNMRKKQYNYYRDQLLTFKEGEV
                     EWKALGEIGEFIRGKRFTKADYVEDGGISVIHYGEIYTRYGVYTTHSLSQVRADMAAS
                     LRYAKHGDVVITDVGETVEDVGKAVAWLGDDDIAIHDHCYAFRHSLNPKFISYYMQTD
                     SFISEKAKYVARTKVNTLLINGFSKIMIPVPYPKDHEKSLKEQARIVEILDKFDTLTN
                     SITEGLPREIELRQKQYEYYRDLLFSFPKPETVSN"
     gene            complement(4675..6231)
                     /locus_tag="ROI21_004502"
     CDS             complement(4675..6231)
                     /locus_tag="ROI21_004502"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDX5113335.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTSV
                     NKIYDPAAGSGSLLLQAKKQFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTKVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSCYVEAKDNREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQK"
     gene            6414..6635
                     /locus_tag="ROI21_004503"
     CDS             6414..6635
                     /locus_tag="ROI21_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDX5113336.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            6635..7015
                     /locus_tag="ROI21_004504"
     CDS             6635..7015
                     /locus_tag="ROI21_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDX5113337.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            7020..7199
                     /locus_tag="ROI21_004505"
     CDS             7020..7199
                     /locus_tag="ROI21_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HDX5113338.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLAGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            7227..7505
                     /locus_tag="ROI21_004506"
                     /pseudo
     CDS             7227..7505
                     /locus_tag="ROI21_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pdcB"
     gene            7519..>7607
                     /locus_tag="ROI21_004507"
     CDS             7519..>7607
                     /locus_tag="ROI21_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113339.1"
                     /translation="MSFSPDSRLTMKALEMAWETRGKPVGVMF"
BASE COUNT         1963 a         1689 c         1718 g         2237 t
ORIGIN      
        1 cattcaaaat cattatggcc gcgatctatt tctggctgtt ctctatcact tttggcggca
       61 tcgtcgctca tgggtaaggg gagtatatta gccatttgga accccacggt ctcttgcggg
      121 tttaattaag aacccgcaag aaaaacacga attgggctat atttttccgc ctacgccttt
      181 aaacttctca ataaacgaga cgattttctg gaaaactgcc tgttttttcg ttttatattg
      241 cgggtttaac ggactaagtt ttggtaatgt ctcgtttaat tctgtgccat tttcggtggc
      301 gtattcgcgt tttaaagacg tgcgaatata gcgtttcgcc gcctcttcat tgagattttc
      361 ttctttaatc aacgcttctg cttcacgtag ctgttcgtgt tgagcaaagg taaagaacgc
      421 gtcaatgatg ctggctttgt ctggtaaatc atccaggttc gtttgctgaa taaaatcgac
      481 caccaggccc tctttcgccc ggttccccag gcttgaacga attaagcgtt tgacctcttc
      541 gatcatttcg cccttgcctt tattttgtct gttatgttcg aaaatcagtc caaggatata
      601 atccaggttt atttcctgag acttcagcaa atcgacctca aaaaccacgt catcccagtc
      661 agtggttgat ttctcttttt tctcagcttc tttctcacgg cgctgccagt cgcgaatatc
      721 gttataggca gaacgataat cctgaatctt gcgatcagca gggagacgaa ttgtttgcaa
      781 ttcagcgagc ttttcatcat ccacataatg ttctgctttg aatttttcta ctgcaacagg
      841 atcgctaaga tcgatttgtt gcagggcttt cagcgtggca aattcatcat agttttgcag
      901 gatgttctcg gtacgcaggt attcaccaaa cagttttacg aagtctttct tctctttttc
      961 actttcaata ctggcagggt cagggaaccg ttgttccagt tctgcaacta ctgccataaa
     1021 gccgcgctta gcttcaccag tagcagcatc agtaaagcct tccatatact ctgcataact
     1081 cttttctaac actacatttt tggtgttttt gtcaccaaac agcgttatgg catcaatagt
     1141 tgagcgttcc agatcccgga aagtgacgat attaccgaag gttttagtgg cgtcataaat
     1201 gcggttggtg cgggaaaatg cctgcatcag gccgtgaaaa cgtaagtttt tatcgacgaa
     1261 tagcgtgttc aatgttggag catcgaagcc ggttaagaac atccccacga caattagcag
     1321 atcgatatcc tgatttttaa cccgctgggc taaatcacga tagtagttct gaaaaccgtt
     1381 actgtcggtg ctaaagttag ttttaaaatg gctgttatat tcacgaattg cagcgtccag
     1441 aaactcttta gcactgctgt ccattgcgct ggtatcaaaa gtttcatcgg aaatctcacc
     1501 aatggcattt tgttcttcat tggcggcaaa ggagaagatt gtcgcaatac gcagcggttt
     1561 ataggtagcc gatttattag cggcttcttc ttgtaaccgt ttaaacgtcg cataataggc
     1621 tttcgcggca tccacgctgc tcactgccaa catagcatta aaaccttttg agccagggaa
     1681 ggtacggtga gttttctggc ggaaattatt cagaatatat tgcgtaattt cctgtatacg
     1741 catgggatga agaaacgcct gctgattttc agccgcactc agttttttct cgtcggtttc
     1801 tgtctctaaa gacttaaact gtggccgcac atcgttgtag tccaccttga atttaagcac
     1861 tttttcatct cgaatcgcat cggtaataac atatgaatgc aattcacgac caaatacgct
     1921 ggcggttgtt tctgagccta aggcgttttc cgggaaaata ggggtaccgg taaaaccaaa
     1981 ctgataatag cgtttgaatt tcttcttcag gtttttctgc gcttctccaa actggctgcg
     2041 gtggcattca tcaaatataa acaccacttg ctgattgtat acaggcaggt cgctttctgc
     2101 tttcatcagg ttattgagtt tctgaatagt ggtgacgata attttgttat catccttatc
     2161 cagatttcgt ttaagacctg cggtattttc cgatccattg acactgtctg gcgaaaaacg
     2221 ctgatattcc ttcatggtct ggtaatcgag gtctttcctg tcgaccacaa agaagacttt
     2281 atcaataaag tccagttctg ttgccagacg cgcggcttta aagctggtca gggttttacc
     2341 agaaccggta gtgtgccaga taaagccacc actttcgggg gtagaccagt ttttcgcttt
     2401 ataggagctg ttgattttcc ataagatacg ctcagtggcg gcaatctggt acggtcgcat
     2461 caccagtagc gtctggctac tgtcaaaaac gctgtagttc accagaacat tcagcagagt
     2521 atgtttctgg aaaaaggtag cggtaaagtc tttgaggtct ttaatcagcg tgttgtctga
     2581 ttttgcccaa ttcatggtga agtcaaaact gtttttatcg cgctttgtcg tgttggcaaa
     2641 ataacgggta tcggtgccgt tagaaatgac aaacagttgc agatacttaa acagggaatt
     2701 ttcgctgtta aaactctctt tactgtaacg atgtatctgg ttgaaagcct cacgaatcgc
     2761 caccccgcgt ttttttagtt cgatttgcac cagcggtaaa ccattaacca ggatcgtgac
     2821 gtcataacgg ttagcatgag aacccgtctg ttcaaactgc tggataatct gcaccttatt
     2881 gcgcatgaga ttctttttat ctatcaaata gatgttctca agacgctcgt catcaaaaat
     2941 aaagtcgcaa atatagtcga tatggatttt acgggtctta tccagaatgc catcgctcgg
     3001 gttatccaga tactgctccg tgaaacgccg ccactcgctg tcattaaaca ccacaccatt
     3061 gaggttctga agctgttccc gaacattggc cagcatcgcc gactgtgatt ttacggatat
     3121 aaattcatag ccctgattcc gcaggtcctg aatcagttct cgttccaggt ccgattcgct
     3181 ctggtagctg tcgcctgttg gctcagcttt gatgtactta tcaaggacga taaagttatt
     3241 ggattcagca atggtgtgtg tctgatgagt catagcgcat cctttgtgcc gtctggcaag
     3301 ggccggaagg gagttaaggg tgacttccgg cacgtaaaaa atagtctata tacagaccgg
     3361 atgttaaggt ggcccggtcg gtagcaacgg tcaattaatt actgacagtt tcaggttttg
     3421 ggaaactgaa cagtaaatca cggtagtatt cgtattgttt ctggcgcaac tcgatttcac
     3481 gcggaagacc ttcggtgatg gagttagtca gtgtgtcgaa tttgtcgagt atttcgacaa
     3541 tgcgagcttg ttccttaagt gatttttcgt gatctttagg atatggaacc ggaatcataa
     3601 tttttgaaaa accattaatg agtagtgtat taacttttgt tctggctaca tactttgctt
     3661 tttcagaaat aaacgaatcg gtttgcatgt aataggaaat aaattttgga ttcaaagaat
     3721 gtcgaaaagc ataacagtga tcatgaatag cgatatcgtc atccccaagc catgccactg
     3781 ctttaccaac gtcttctaca gtctccccca cgtcagttat cacgacatct ccatgtttgg
     3841 catagcgtaa tgacgctgcc atatcagctc taacctgtga taacgaatga gttgtgtaaa
     3901 caccatatcg tgtatatatc tcaccataat ggattacact gataccacca tcttctacat
     3961 aatctgcttt agtaaaacgt tttccacgaa taaactcacc aatttccccc aaagctttcc
     4021 actcaacctc accttcttta aaagtcagca attggtcgcg atagtagttg tactgttttt
     4081 tacgcatgtt aagctcagcg gtaagctcag cggtaagctc agcggtaagt gcagtaaact
     4141 tatccagaat ccgaacgatt tcagactgga tggcaaggga cttttccgga ttatccgggc
     4201 aggggatggg gatcttaata tttttgacaa tctgcgcatt tatgtttgtc tgggaccctg
     4261 ttccaaggga tttgatatat gtgtattggc tacacaagaa gtgaaataca tatctataat
     4321 gagcaacttc ttcattaagt tgaatatttg cgcacgcttg atttgttgtc attggaattt
     4381 tgtttatgcc gattttcccc acagttgccc catacatagc aacaatgaca caattctttg
     4441 gtatccattt tgcactagag tttttaactc cagactcagt tatttttacc tcggtatccc
     4501 atatatcaca aaagtttact tcttgagttc tcaaccaagg aatgtcgcca tcataaaatt
     4561 ctgatacgcc agttttaggg gttcctccag atgatatctt tatagaaata tcctcaaggg
     4621 ttttccactc aacctcaacc ccatccagca atttttccag ataactcaac tcgctcattt
     4681 ctgcacctcg cagccttcaa tttcagccac aatcgcatca atatctttac gcaactggtc
     4741 gattttgctg accgtagttt taagctctgc atttagctca gcaatattga taatttcgcg
     4801 gttatctttc gcttctacat agcagctcac cgacaggtta tagtcattag cgacaacggt
     4861 ctcaaacgcg acagatttcg ccagatgagc aacatcttcc ttgctggcaa atacctgcat
     4921 aatctgttcg atatgggcat cggtcaggat attgttgttg gtctcttttt tgaatagttc
     4981 gctggcatca ataaactgaa ctttggtatc cgttttatgt ttagacaaca ccagaatatt
     5041 gacggcaatg gtggtgccaa agaacaggtt cggtgcgagt gaaatcacgg tttcgacata
     5101 gttattgtca accagatact gacggatttt ctgctccgcg ccgccacggt aaaaaatgcc
     5161 cgggaagcag acaatcgcag cacgaccttt ggcagaaaga tagttcagcg catgtaatac
     5221 aaacgcaaag tcagctttgg atttgggggc cagaacgcca gccggggcaa aacgttcatc
     5281 gttaatcagc gtcgggtcat cgctgccaat ccatttcacc gaatacggcg ggttagaaac
     5341 gatggcatca aacggttttt catctctgaa gtgcggttca gtcagcgtat tgcccagctt
     5401 gatatcaaac ttgtcgtagt tgatgttgtg caaaaacatg ttcatacgcg ccaggttata
     5461 ggtcgtatgg ttgatttcct gaccaaaaaa accttcttcg atgatatggt tatcaaactg
     5521 ttttttcgcc tgcaacaaca gtgagccgga acccgctgcc gggtcgtaga ttttgttaac
     5581 gctggtctgc ccgtgcatag ccagttgtgc aatcagcttg gagacgtgct gcggtgtaaa
     5641 gaactcaccg cctgacttac cggcatttgc cgcatagtta gaaatcagga actcataggc
     5701 atcaccgaac aggtcaatct gatgttcgtt gaagtcacca agttttaacc cttcaacccc
     5761 tttcagaacc gcagccaggc gggcattttt atctttaacg gtgttaccca ggcggttact
     5821 ggtggtatcg aaatcagcaa acaaaccttt gatgtcagct tctgaaggat aaccgtaagc
     5881 agaactttcg atagcaacga agatgctgtt taaatctgca ttcaatctgt cattggtatt
     5941 tgctttcgca gctacgttgc agaaaagctg gcttgggtag atgaagtagc ctttggtttt
     6001 gatggcatcg tctttaatgt catcagtaat tacgctgtca tccagtttcg cataacagat
     6061 actgtcatca ccggcttcaa tataactgga aaaattttcg ctgataaaac ggtagaaaag
     6121 tgcgcccaga acgtattgct taaaatccca tccatcgacc gaacccctga catcgttagc
     6181 aatttgccag atttgacgat gaagctctgc acgttgttga atacttgtca ttttcatcca
     6241 cttatttcag gcttatgtaa ttggcggtga ttctacagca acttggatgc tttagcagtt
     6301 cggacattag gctacgaatg acctgcctag aggtttgtta agccgcaaag tgctggtgct
     6361 ttatgcctgt gaagtttata attgtgtaca cataacgagt acacgaggtg tttatgcaat
     6421 ccattaactt ccgtaccgcg cgcggcaacc tttctgaagt gctcaacaat gttgaggccg
     6481 gggaagaggt tgaaatcacc cgcagaggcc gtgagccagc agtaattgtc agcaaggcta
     6541 ctttcgaagc ctacaaaaaa gcggcgctgg atgctgaatt tgcatccctg tttgacaccc
     6601 tggactccac caacaaggaa ctggttaacc gataatgagg catatatcac cggaagaact
     6661 tattgcgctt catgatgcga atataaaccg ctacggcggc ctgccgggaa tgtctgatcc
     6721 gggcagggca gaggccatta tcgggagagt tcaggccaga gttgcctacg aagagatcac
     6781 cgaccttttc gaagtctccg ccacctacct ggtggctaca gcgagagggc atatattcaa
     6841 tgatgccaat aagcgtaccg cgctaaacag tgcgctgtta tttctacgcc gtaacggggt
     6901 gcaggtattt gattcacctg aactggcaga ccttaccgta ggggctgcga ccggagagat
     6961 atctgtatct tctgtcgccg acacgttacg tagattgtat ggttctgcgg agtagattaa
     7021 tggcacgcaa atacaacaaa ttgtcccgtg aagcgttaaa gatgcttctt gatggcgtga
     7081 gtcgccgcga ggtaaagcaa tacctggctg gtaagcaaat tggtgccagg accgctattg
     7141 ctgtgttatg ccgtcaggaa atggttgtgc ttaaacagag aatgccgggc agcagataaa
     7201 gcccaatcag tgatgaaagg tgtgatgtga aagccgtaat tactcccttt gtacaaaaag
     7261 agcttggcgt cgccacattc aaagtggatc aggaagtcag aaagctggtg gaggctggcc
     7321 gtaaatttat tatggagccg gtaccgcgtg agttaatcga gcacatggac gacggcctcg
     7381 ttgtttccga gcaaactatg gcaacaaatg aggcgttgca gccgtttttt aacagcgatg
     7441 aactgtttcg ccgtattggt ggaattgact cgctggtagc gtggttgcgc aggaaagagg
     7501 ggcaggtcag gctgggccat gtcgttctcg ccggacagca ggcttaccat gaaagcactg
     7561 gaaatggcat gggaaacccg tggtaagccc gtcggggtga tgttcca
//
LOCUS       DAPOZO010000165         7576 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPOZO010000165 DAPOZO010000000
VERSION     DAPOZO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.166"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..434
                     /locus_tag="ROI21_004508"
     CDS             <1..434
                     /locus_tag="ROI21_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDX5113340.1"
                     /translation="FVQFPAEKIEAVCHAIAAHSFSAQIAPLTTEAKIVQDADRLEAL
                     GAIGLARVFAVSGALGLALFDGEDPFAQHRPLDDKRYALDHFQTKLLKLPQTMQTARG
                     KQLAQHNAQFLVEFMAKLGAELAGENEGIDHKVIDAFSPAG"
     gene            501..1919
                     /gene="dcm"
                     /locus_tag="ROI21_004509"
     CDS             501..1919
                     /gene="dcm"
                     /locus_tag="ROI21_004509"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDX5113341.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            1900..2370
                     /gene="vsr"
                     /locus_tag="ROI21_004510"
     CDS             1900..2370
                     /gene="vsr"
                     /locus_tag="ROI21_004510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDX5113342.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2359..3279)
                     /gene="yedA"
                     /locus_tag="ROI21_004511"
     CDS             complement(2359..3279)
                     /gene="yedA"
                     /locus_tag="ROI21_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDX5113343.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            3452..4369
                     /locus_tag="ROI21_004512"
     CDS             3452..4369
                     /locus_tag="ROI21_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDX5113344.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            4448..4630
                     /locus_tag="ROI21_004513"
     CDS             4448..4630
                     /locus_tag="ROI21_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDX5113345.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            4868..6562
                     /gene="yedQ"
                     /locus_tag="ROI21_004514"
     CDS             4868..6562
                     /gene="yedQ"
                     /locus_tag="ROI21_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDX5113346.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRSDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(6559..7374)
                     /locus_tag="ROI21_004515"
     CDS             complement(6559..7374)
                     /locus_tag="ROI21_004515"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDX5113347.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
BASE COUNT         1846 a         1881 c         2082 g         1767 t
ORIGIN      
        1 agttcgtgca atttccggcg gagaaaattg aggccgtttg tcatgccatt gccgctcaca
       61 gtttcagcgc gcaaatcgcc cccttaacaa cggaagctaa aatcgtccag gatgcggatc
      121 ggctggaagc cctgggggcg attggcctgg cgcgcgtatt tgccgtttca ggggcattgg
      181 gcttggcgct gtttgatggc gaagatccgt tcgcacagca tcgcccgctt gatgataaac
      241 gctacgcgct ggatcatttc cagacaaagt tactgaaatt accgcaaacc atgcaaaccg
      301 caagaggcaa gcagctggca cagcacaacg cgcaattttt agtcgagttt atggcgaagc
      361 tcggtgccga actggcgggg gagaatgaag gtatcgatca caaggtgata gatgcgtttt
      421 cacctgctgg ctgagcgcgt ggccctaaac ggcagtaatt atgttaacct gtcggccatc
      481 tcagatggcc ggttaaatct atgcaggaaa atatatcagt aaccgattca tacagcaccg
      541 ggaatgccgc acaggcaatg ctggagaaac tgctgcaaat ttatgatgtt aaaacgctgg
      601 tggcgcagct taatggtgtg ggtgagaatc actggagcgc ggcaatttta aaacgtgcgc
      661 tggcgaatga ctcggcatgg caccgtttaa gtgagaaaga gttcgcccat ctgcaaacgt
      721 tgttacccaa accaccggca catcatccgc attatgcgtt tcgctttatc gatctatttg
      781 ccggaattgg cggcatccgt cgcggttttg aatcgattgg tggacagtgc gtgtttacca
      841 gcgaatggaa caaacatgcg gtacgcactt ataaagccaa ccattattgc gacccggcga
      901 cgcatcattt taatgaggat atccgcgaca tcaccctcag ccatcaagaa ggcgtgagtg
      961 atgaggcggc ggcggaacat attcgtcaac acattcctga acacgatgtt ttactggccg
     1021 gtttcccttg tcagccattt tcgctggctg gcgtatcgaa aaagaactcg ctcgggcggg
     1081 cgcacggttt tgcctgcgat acccagggca cgctgttctt tgatgtggtg cgcattatcg
     1141 acgcgcgtcg tccggcgatg tttgtgctcg aaaacgtcaa aaacctgaaa agtcacgacc
     1201 agggtaaaac gttccgcatc atcatgcaga cgctggacga actgggctat gacgtggctg
     1261 atgcagaaga taacgggccg gacgatccga aaatcatcga cggcaaacat tttctgccgc
     1321 agcaccgtga acgcatcgtg ctggtgggtt ttcgtcgcga tctgaatctg aaagccgatt
     1381 ttactctgcg tgatatcagc aaatgtttcc ctgcacagcg agtgacgctg gcgcagctgc
     1441 tggacccgat ggtcgaggcg aaatatatcc tgacgccggt actgtggaag tacctctatc
     1501 gatatgcgaa aaaacatcag gcgcgcggta acggcttcgg ttatggaatg gtttatccga
     1561 acaatccgca aagcgtcacg cgtacgctgt ctgcgcgtta ttacaaagat ggcgcggaaa
     1621 ttttaatcga tcgcggctgg gatatggcaa cgggtgagaa agactttgac gatccgctga
     1681 atcagcaaca tcgcccacgt cggttaacgc ctcgggaatg tgcgcgctta atgggttttg
     1741 aagcgccggg agaagcgaaa ttccgcattc cggtttcgga cactcaggct tatcgccagt
     1801 tcggtaactc ggtggtcgtg ccggtctttg ccgcggtggc aaaactgctt gagccaaaaa
     1861 tcaaacaggc ggtggcgttg cgtcagcaag aggcacaaca tggtcgacgt tcacgataag
     1921 gccacccgca gcaaaaatat gcgcgcgatt gccacgcgtg atacggcgat tgagaagcgc
     1981 ctcgccagtt tgttaactgg gcagggcctg gcgtttcgcg ttcaggacgc cagtctgccc
     2041 ggacgcccgg attttgtcct tgatgaattt cgctgtgtga tatttgccca cggttgcttc
     2101 tggcaccatc accattgcta cctgtttaaa gtccccgcca cgcgaacaga tttttggctg
     2161 gagaagatag gtaaaaatgt cgagcgcgat cgtcgtgata tcagccattt gcaggaactc
     2221 ggttggcgcg tattgattgt ctgggaatgt gcgttacgcg ggcgcgagaa gctgacggat
     2281 gcggcgctta ccgagcgtct ggaagagtgg atctgcggcg aaggtgccag cgcgcagatc
     2341 gacacgcagg ggattcattt actcgcttga tgcatccgga ataactggcg cgacaacggg
     2401 tttggccggg aagagatatt ttcccaacgt gaccagtacc accgcgaaaa caattacgcc
     2461 gagcgccagc cattcaatct tcgacagcgt ttcgccaccc agccccgtac ccagcaagac
     2521 cgcgaccact gggttaacgt aggcgtagct ggtggcgaga gccggactga cgttgcggat
     2581 taaatacata taagcgttaa tggcgataat cgaaccaaac agcgccagat agccgaccgc
     2641 aaggaagcct gaaagggaag ggagcgccgt cagtttttca cccgcaatca tcgacgcaat
     2701 cattaacacc acgccagccg ccagcatctc aatcgcaccc gccatcatcc ctacaggtaa
     2761 ggtaatgcgc gagccataaa ccgagccaaa cgcccagcta atcgagccga ttaaaatcag
     2821 aatcgcgccc cacggattgc cgcttaaatt tccaccgcta ttgagcatga tgattccggc
     2881 aagcccaatg gcgataccca cccattccag tttgcgcgtt ttaatgccaa acaggcggct
     2941 gaagcacagg gtaaagaggg gcacggttgc aaccacaacg gcggcgatgc cggaaggaac
     3001 attttgatgt tcggcaaccg tcaccatgcc attaccgaca gccagcaata acaggccaat
     3061 cagcgcggca ttgagcagcg gacgtagcgg gggaagtttg tgtccgcgca gtagcaaaaa
     3121 tgccagcaat aaaataccgg ctgccaggaa tcgaacgccc gccatcatta acggaggcca
     3181 gctttccacg ccaatccgaa tgacaaaata ggttgagccc caaatgatat acaacgcaaa
     3241 cagcgcgcca aaaagcggta acaactggcg gaaacgcata atccctcacg gctgaaataa
     3301 aaaggtggtt catagtaaac gtgaaaatca ttctgctggc gagagatata attgcacttg
     3361 attgttaaat aaatgttgac ctgtgaagca agtatcagag ctgccgtttt tgcttcatac
     3421 ttacaccctt caacaataaa aatgagaggg aatgcttttg gccgggagta gtttactgac
     3481 gttgctcgat gatatcgcca cactgctgga cgatatctcc gtgatgggca aactggcggc
     3541 gaagaaaacc gccggtgtat taggggatga cttatcgctc aatgcgcaac aagtttcagg
     3601 cgtgcgcgcc aaccgggaac ttcccgtggt ctggggcgtg gcgaaaggat cgctgatcaa
     3661 taaagtgatt ctggtgccgc tggcgctgat catcagtgcg tttatcccgt gggcgattac
     3721 gccactgttg atgattggcg gcgcgtttct ctgctttgaa ggggtagaga aagtgctgca
     3781 tatgctggag gcgcgtaaac ataaagaaga tccggcgcag agtcagcagc gtctggagaa
     3841 gctggcggcg caggatccgc tgaagtttga aaaagacaaa ataaaagggg cgattcgtac
     3901 cgattttata ttgtctgcgg aaatcgtcgc catcacgctg gggattgtgg ccgaagcgcc
     3961 gttgcttaat caggtgctgg tgctttcagg catcgcgctg gtagtgaccg tgggcgtcta
     4021 cggtctggta ggggttatcg ttaagattga tgacctgggg tattggctgg cggaaaaatc
     4081 cagcgcgctg atgcaggcat taggtaaagg gttattgatc attgcgccct ggttgatgaa
     4141 ggcgttatcg attgtcggca cgctggcgat gttcctcgtc ggcggtggga ttgtggtaca
     4201 tggaattgcg ccgctgcatc acgccattga acatttcgcc gggcagcaaa gtgcagtggt
     4261 ggcgatgata ttaccgactg ttttaaatct gattcttgga tttatcatcg gcggcatcgt
     4321 ggtgctggga gtgaaagccg tagcgaaaat acgcggtcag gcacattaat actcgcacag
     4381 gctattatgc aaaattcgcc atcttcaact aaggtgaaca agtttcactc gaaaaaagga
     4441 ggcgagtatg agctttatgg ttagtgagga agttacggtt aaagagggcg gcccgcggat
     4501 gattgtcacc ggatactcca gcggtatggt tgagtgtcgt tggtatgacg gttacggggt
     4561 caagcgggaa gcttttcatg aaaccgagct tgttccgggg gaggggagtc gttctgcgga
     4621 agaagtttga aagggtatca ttaacacccg accggatgtg gcgctcacgt cgcatccggt
     4681 cgggtgtgcc agtgtctgca ctgaattgcc tgatgcgacg ctatcgcgtc ttatcatgcc
     4741 tacgttataa aactatcgat gtctgtcgcg tcttatcatg ccaccattat aaaattatcg
     4801 ctgttttgcg gtacgctatc gccaggttat gccagaatca taaaaaagca ggttgggagt
     4861 cgtcagggtg cagcacgaga caaaaatgga aaaccagagc tggttgaaaa aactcgcacg
     4921 ccgcctgggg cctggtcatg tcgttaatct ctgctttatc gtggtattgc ttttttccac
     4981 cttgctcacc tggcgtgaag tggtggtgct ggaagatgcc tatatctcca gccagcgtaa
     5041 tcatctggaa aacgtagcca acgcgctcga taagcatttg cagtataacg tcgacaaact
     5101 gatctttttg cgtaatggca tgcgcgaagc tctcgtagcg ccactggatt tcacctctct
     5161 gcgtaatgcc gtaaccgagt tcgaacagca tcgcgacgag cacgcctggc aaatcgaact
     5221 caaccgacga cgcaccctgc cagtcaatgg tgtgtcggat gcattagtca gcgaggggaa
     5281 tctcctgtct cgcgaaaatg aaagcctcga caatgaaatt accgctgcac tggaagttgg
     5341 ttacttgctg cgactggcac acaactcctc gtcgatggtt gaacaggcga tgtatgtctc
     5401 gcgtgccgga ttttacgttt cgacgcagcc gaccttgttt acgcgcaatg taccaacgcg
     5461 ttattacggc tatgtcaccc aaccctggtt tgtcggccat tcgcaacgag aaaatcgtca
     5521 ccgcgcggta cgctggttca cttcgcaacc ggaacacgcg agcaatactg aaccgcaggt
     5581 taccgtcagt gttccggtag acagtgaaaa ctactggtat ggcgtgctgg ggatgagtat
     5641 tcccgtgcgt accatgcagc aatttttaag aaacgccatc gataaaaacc tcgatggtga
     5701 gtatcagctc tatgacagta agctgagatt attgacctct tccaatcctg accatccaac
     5761 agggaatatt tttgatcctc gtgaactggc cttgctggcg caggcaatgg aacatgacac
     5821 gcggggcggc attcgtatga acagtcgcta tgttagctgg gaacgtctgg accatttcga
     5881 cggtgtgctg gtgcgcgtcc atacgctaag cgaaggcgtg cgcagcgatt tcggcagtat
     5941 cagcattgca ttaaccctgc tgtgggcgct ctttaccacc atgttactca tctcctggta
     6001 tgtgattcgc cggatggtca gcaacatgta tgttttgcaa agctcgttgc agtggcaggc
     6061 gtggcacgac accttaacgc gtttatataa ccgtggcgca ctgttcgaaa aagcccgtcc
     6121 gctcgctaaa ttgtgtcaga cgcaccaaca tcctttttct gtcattcagg tcgaccttga
     6181 ccattttaaa gcgattaatg accgctttgg tcatcaggcg ggcgaccgtg ttctttctca
     6241 tgctgccgga ttaattagca gttccttgcg agcgcaggat gttgccgggc gggtcggtgg
     6301 tgaggagttt tgtgtgattc tgccaggcgc gagcctgacg caggctgcgg aggttgcaga
     6361 acgtattcgc ctgaagctaa atgaaaaaga gatgttgatc gccaagagta cgacgatacg
     6421 catcagtgcc tcgctggggg taagcagcag cgaggaaacc ggtgattatg attttgaaca
     6481 actccagtca ctggccgacc gtcgacttta tctcgctaaa caggccgggc gcaatcgggt
     6541 attcgcgagc gataacgctt aatgggcgga gaaaaaatgg tccagccctt cacgccagcc
     6601 ttccggtcct tcacgctgcg ttcgccagac gcgggccgga tcctcatcat gcagatgcac
     6661 cccttcacgg tttaaccctt tcacaatcac cgcgtaatcc attacctcca gtaagggcgc
     6721 atcgtttggc ccatcgccca ggccaagtgt ggttgggcgt ttgcctgacg attgttgata
     6781 ggtcgcgata atccagttgg cagcctgatc ttttccggca gaggcatcca ggacgtgcca
     6841 gaagcgcgca ccctgcataa actgcaagcc cagttcgttc agacgagcgg taaattgtgc
     6901 catacgctcg tcactgtcgc gccagattag cgttaccgac gcctcatgta gctgcgtcag
     6961 cgccgcctgg ctacggctta atcccgtcca ttcggcgatg gttgcatcgt cgacatcatc
     7021 aaaagttgtg aatttaaaat gttctttctc gcgtagcgta tttaaaacct ggctgatttc
     7081 gccatggcta atacctgaga tgatgcgtgg aaaaccgtct atatcctgcc attgctcagc
     7141 aagctggatc actgcgccat tttcggcaat cagcggtaaa ccttgtagcc ccaacgtttt
     7201 ttgcaagtac agcatttcgg ctgatgtctt actgctacag agaatgacgg gaacattcgc
     7261 ttcgcgtaaa cggctgagcc agggggcagc tggttgccag tcataactat gactgtccag
     7321 cagggtgcca tcaagatcgc taaaaaccag tagtggttgt tgaattgaaa gcatggttgc
     7381 gcagtactcc ccttaccagg atgattctca aaacagcaca aattatcaaa gatgattttt
     7441 tcgggagcga tcccgttgac gccagagcca ccaaggggtg acgcgcgtca catttctatt
     7501 cataagtagc gttaatcatt catatcacga atattttctt gtcagcgaaa aaaattgcgg
     7561 ataaggtgat gaacac
//
LOCUS       DAPOZO010000166         7540 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPOZO010000166 DAPOZO010000000
VERSION     DAPOZO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.167"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..887
                     /locus_tag="ROI21_004516"
     CDS             <1..887
                     /locus_tag="ROI21_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDX5113348.1"
                     /translation="SDDLLFKKLFSPSKLMTIIGEDIPLISEKQSLSKVLLNDKNNEL
                     SDGTNFWDKNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPNSNSTYLEVALNDNI
                     RSDPLWPKAVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFNSLRALGHEKENSL
                     KRIINSFNSELMGEMSNNNITDHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQ
                     RESNSDSALYTLLKNYADRFKKYSAEEQYEIDFQHRQVNRNCYLDKYGDANINDYYRD
                     LEIKHSQPQNRASGKRVS"
     gene            1268..2239
                     /locus_tag="ROI21_004517"
     CDS             1268..2239
                     /locus_tag="ROI21_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HDX5113349.1"
                     /translation="MKKITPLCFISLLLSLPFIIFYQPWVNALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLKRSAEYIKEVFVSSGARVTAQDVPITGGPYKNIVADYGP
                     ADGPLIIIGAHYDSASSYENDQLTYTPGADDNASGVAGLLELARLLQQQVPKTGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVRLMIALEMIGYYDSAPDSQNYPYPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQLKAALLSSQDLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDTADRLNYQKMAQVVDGVITLLYNSK"
     gene            2342..2845
                     /locus_tag="ROI21_004518"
     CDS             2342..2845
                     /locus_tag="ROI21_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDX5113350.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(2912..4369)
                     /gene="nuoN"
                     /locus_tag="ROI21_004519"
     CDS             complement(2912..4369)
                     /gene="nuoN"
                     /locus_tag="ROI21_004519"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDX5113351.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(4376..5905)
                     /gene="nuoM"
                     /locus_tag="ROI21_004520"
     CDS             complement(4376..5905)
                     /gene="nuoM"
                     /locus_tag="ROI21_004520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDX5113352.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(6136..>7540)
                     /gene="nuoL"
                     /locus_tag="ROI21_004521"
     CDS             complement(6136..>7540)
                     /gene="nuoL"
                     /locus_tag="ROI21_004521"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDX5113353.1"
                     /translation="GLCSYLLIGFYYTDPKNGAAAMKAFVVTRVGDVFLAFALFILYN
                     ELGTLNFREMVELALAHFADGNNMLMWATLMLLGGAVGKSAQLPLQTWLADAMAGPTP
                     VSALIHAATMVTAGVYLIARTHGLFLMTPEVLHLVGIVGAVTLLLAGFAALVQTDIKR
                     VLAYSTMSQIGYMFLALGVQAWDAAIFHLMTHAFFKALLFLASGSVILACHHEQNIFK
                     MGGLRKSIPLVYLCFLVGGAALSALPLVTAGFFSKDEILAGAMANGHINLMVAGLVGA
                     FMTSLYTFRMIFIVFHGKEQIHAHAVKGVTHSLPLIVLLILSTFVGALIVPPLQGVLP
                     QTTELAHGSMLTLEITSGVVAVVGILLAAWLWLGKRTLVTSIANSAPGRLLGTWWYNA
                     WGFDWLYDKVFVKPFLGIAWLLKRDPLNSMMNIPAVLSRFAGKGLLLSENGYLRWYVA
                     SMSIGAVVVLALLMVLR"
BASE COUNT         2148 a         1964 c         1834 g         1594 t
ORIGIN      
        1 attcagatga tctcctgttc aaaaaactat tctccccttc gaaattaatg acaattatcg
       61 gtgaggacat tcctcttata tcagaaaaac agtcgctttc aaaggtactt ttaaatgata
      121 agaataatga actgagcgat gggacaaact tctgggataa aaatcgtcaa ttaaccacag
      181 atgaaatagc ttgctatctt aaaaagatag ccgcaaatgc aaaaaatact caagtcaatt
      241 atcctactga tttctacctc cccaattcca acagcactta cctggaagtc gctctcaatg
      301 ataatattag gagcgatcca ttatggccga aagcagtcca gttattcccc ataaatactg
      361 gtggacactg gatattagtt tcgctacaaa aaatagttaa tgaaaaaaat aacacacaac
      421 aaataaaatg catcatattc aattcattac gtgcactagg ccatgaaaaa gaaaattcac
      481 ttaagcgtat cattaatagt tttaattctg aactcatggg agaaatgtcg aataataata
      541 taacagatca tttaactgaa cccgagataa tatttttaca tgccgatctt cagcaatact
      601 taagccaaag ttgcggtgca tttgtgtgca tggcagccca ggaagtgatt gaacaaaggg
      661 aaagcaattc cgacagcgcc ctctatacgt tattaaaaaa ctatgctgac agatttaaaa
      721 aatattcagc agaagagcag tacgaaattg attttcaaca tcgacaggta aacagaaatt
      781 gttatttaga taaatatggc gatgcaaata tcaatgatta ttatagagac ttagaaataa
      841 aacactcaca accccaaaat agagcatcag gcaaaagagt aagttaacat cattgttaat
      901 taattcgacc agtcagcaaa tatgctgact ggtcgaacat agtttgctaa aaagtcatca
      961 ctgactgatg tcagtcacac cacccgccag ttcaatctgc gggtatttcc agcgtatcgc
     1021 ggcacgcgat tgctggtctg ttgatgtagt ggattgtagt tgctgatttt tcttacccta
     1081 agatttcaat tcaatctgcc aataaataac ataattatat tttttcctca tatgaattcc
     1141 tttttaatat agcgaactca ctgtaaaaaa aacacttttt gtatatctct accaaccgta
     1201 ataaaataaa cactcacagc tcattataaa taataatgaa ataacgcctt ttattgataa
     1261 tttacatatg aaaaaaataa cacctctttg ttttatatca ttactactat cacttccttt
     1321 tattattttt taccaaccct gggtcaatgc attaccgcca acgccccgac acgcaagtcc
     1381 tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatcccc gtagtgccga
     1441 caatattgat aatctgaaaa ggtccgcaga gtatataaaa gaggtctttg tcagtagcgg
     1501 tgccagagtt accgcgcagg acgtccccat tacgggtggc ccctacaaaa acattgttgc
     1561 tgattatggt cctgccgatg gaccgctgat tattattggt gcgcactatg actctgccag
     1621 cagttatgaa aacgatcaat tgacctatac gccgggcgcg gatgataacg ccagcggcgt
     1681 ggcaggatta ctcgaactgg cacgtttgtt acagcagcaa gtaccgaaaa ctggcgtgca
     1741 gctggtcgcc tatgcgtcgg aagaaccgcc cttctttcgt agcgatgaaa tggggagcgc
     1801 ggtacatgca gcttcgcttg agcgtccagt gagattaatg atagcgctgg agatgattgg
     1861 ctattacgac tctgcgcctg atagtcagaa ttacccttac ccggcaatgt cctggcttta
     1921 tcccgatcgg ggagacttta ttgccgtggt aggcagaatg caggatatca acgccgttcg
     1981 tcagctaaaa gcggcattgt tgtcatctca ggatttatct gtttattcta tgaatgcccc
     2041 agggtttatt cccggtattg atttctctga tcaccttaat tattggcaac acgatattcc
     2101 cgccgtaatg attaccgaca ccgcttttta tcgtaataaa caataccact tgcccggtga
     2161 taccgcagac agattgaatt atcagaaaat ggctcaggta gtggatgggg ttataacttt
     2221 gttatacaac agtaaataat aaaaatttct cgaggcgggt ccagaaaaag cgacatgtac
     2281 attttatttt ccctcgtctg ttttaagatg cgaaacatga aattcaacgg aggaaaataa
     2341 aatgggaatg attggctatt ttgcagaaat tgactcagaa aagattaacc aactgcttga
     2401 gtctactgaa aaacccttaa tggacaatat tcatgacacg ctttcaggat tacgccgact
     2461 ggatattgat aaacgctggg attttttaca ttttggcctg acaggcacct ctgcttttga
     2521 tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc tcgaagatgg
     2581 catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa tcgatcgcct
     2641 ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaaacgac tcaatgaaat
     2701 ggagatttac cctggtgtga ctttttcaga agagttagag gggcaactct ttgcttcgat
     2761 catgcttgat atggaaaagc tgattagtgc gtatcgacgt atgctgcgac aagggaatca
     2821 tgcgctgacg gttattgttg ggtgatggtg tcagggcacc aaaaaaaacc gccgaacatg
     2881 tcggcggctt tctgacttac aaactgacag cttacatcag cggcatggct aaccgcacaa
     2941 tgctaatcag cggttgtggc catacaccca gcaccagtac caacagtgca gagatcagca
     3001 ccacgatacc acccgcgctg tactgccagt ttgatggtgc atcgcgaccc ggttgttccg
     3061 gggcgtgcag gtataagctc accgccacgc gcaggtagta gtagaggccg attgccgaac
     3121 cgacaaccac ggcacccacc agccaccaca agtgcgcctg gacaccgacc gccagcacgt
     3181 agaacttacc aataaagccc agcgtcatcg ggataccggc cagagacagc atcatcaccg
     3241 tcatcaccgc cgcgaggatc ggacgatgcc agaacagtcc gcggtaagag aacagggaat
     3301 cagcatccgg gccacgatac gggctggaca tcaggctgac cacgccgaac gcgccaaggc
     3361 tgctgaacag ataaccggcc aggtaaaccc ctaccgcttc catcgacatc tcgccggttt
     3421 gcagcgcaat cagcgccacc agcagatagc cgaggtgaga gatagatgag taaccgagca
     3481 gacgtttgat attggtctgg ctcaacgcca tcaggttacc gaagatgata gaggcaaagg
     3541 cgataatcgc cagcaccacg cgaatcgctt cgctgtcacc caccggtgcg tagaggaaca
     3601 gacgcatcac cacgccgaag atagcgattt tgctcgccgt cgccaggaaa gtggaaaccg
     3661 gcgcaggcgc gccctggtat acgtctggcg tccacaggtg gaacggcacc agagagagtt
     3721 tgaagccgag gccaacaatc atcagtccga aacctgccag caacagcggc tcgttgagca
     3781 taccgtcgcc gaggttttta cccagcgcga caaacgacag gtcgccagac tgcgcataca
     3841 ccagcgccat accaaacagc aggaaagaag acgctgcggc agagagaatg gtgtatttga
     3901 tactggcttc cagcgaacgt ttctggcgga aagcgtaacc gaccaggcca aacagcggca
     3961 aagagatcag ttcgataccg aggaacagag acgccagatg gttggcattc gccagcagga
     4021 tcccgcccag cgcggcaatt aacaccaaca ggtagaactc atccttgttg tcgttatagc
     4081 cttcaagcca cgggtaggcg aaagtacagg tggcgaggct cgccaacaat accagcccgg
     4141 tgtaaagcat ggcgaaacca tcaacgcgca tcagcggcgt aacgtccata gcgcccgcct
     4201 ggccgacaaa ccagagcgaa accagcgccg cgttaagccc aataaccgag agcgtagcgt
     4261 tgaggaaatg attgcgtcgc cacgcaatgg agagcatcac aaccaccacc gtcaagccga
     4321 cgatcagcaa cggtagcagt gcgatcaggt tttgtggagt tattgtcatg gcgatttacg
     4381 gccttgtagt agtaacggaa ttaacaaacc actgctggat attgccaatc gcggaatgcg
     4441 aggtatccag aatcggctgc ggatagaagc ccagcagtac cagcagcacc accagcaaca
     4501 ggatcataaa cagctcacgc agcgacatcc ctggcagttc ctggctggca atctggcttt
     4561 tcgctttacc gaagtaagcg cgatgcaaca tcgccagcga ataaacagat gcaaagacca
     4621 gcccaaaggt agagataacg gtaatcaccg ggacaacctg gaagctgccg aacagaatca
     4681 taaattcgcc gacgaagtta ccggtgccag gcatcccaag cgttgccacc gcaaagaaca
     4741 gcgacagtgc tggcagccat ttcatcttgc tccacagacc gcccatcatg cgcatgtcgc
     4801 gggtatggat acgttcataa agctgaccgc agagaataaa cagacccgcc gccgacaagc
     4861 cgtgcgcaat catctggatt accgcgccct ggtaggccaa ctggctgccg gtatagatag
     4921 caatcagcac gaagcccatg tgggaaaccg aggtgtaggc gatcagacgt ttgatatcgg
     4981 tctgggcgaa ggccatccac gcaccgtaga agatgccgat aacacccagc cacatagcga
     5041 ttggcgcgaa ctctgccgac gcgttcggga acagcggtag ggagaaacgc agcagaccgt
     5101 aagcggcggt tttcagcaag atccccgcga ggtcaacgga accggcggtt ggagcctgtg
     5161 agtgcgcatc cggcagccag ccatgcagcg gaaccaccgg cattttcacc gcaaatgcga
     5221 tgaagaagcc cagcatcagc aaatattcca cgccgttaga cattggcgta ttcagcagct
     5281 cttcatagtt gaaggtccag acgccggtcg cattgtagtg aacaaaaacc agcgccagga
     5341 tggcgatcaa catcaccaga ccactcgcct gggtgtaaat gaagaacttg gttgccgccg
     5401 tgatacgcgt tttaccgtca gaggctttat gcccccacag tgcgatcagg aagtacatcg
     5461 gcaccagcat catttcccag aagaagaaga acaggaacat gtcgatggca aggaacacgc
     5521 cgataacgcc gcccaggatc cacatcaggt tgaggtggaa gaagccctgg tatttttcga
     5581 tctctttcca cgaacagagt accgccagca caccgagcag accggtcagc acgaccatca
     5641 gcagcgatag cccgtcaatg gcgagatgga tagagatacc aaaacgcggg atccacggca
     5701 tgtcgaattc agactgccac tgcggaattc cggcggattg cgtcagtgaa taaccgccct
     5761 gcaaccacag ttgcagcgac agcgccagcg tcaatcccat ggtgatcagc gcgatccagc
     5821 gcggcacctt gacgccaaag cgttcggtct gccagcacag gaagccgccg ataaagggga
     5881 ttagtattaa ccagggtaat aacatagcta agtatgtccc ttatatttac tcctgctcag
     5941 gctatctcac tcgccagccc gaatcccggc agtgctcgca atcctcacgt acttaagtac
     6001 gctccggttg ctgtgcgctg gcggtatccg gtctgacttc gccgatgacg cctgtacttc
     6061 aggaacagtt acctaaaatt tattcttagt tcacagaccc ggcgcaacgc cgggtatatt
     6121 cactcagcca acaactcaac gcagtaccat caacagtgcc agcaccacga ccgcaccgat
     6181 gctcatggat gccacatacc agcgcagata accgttctcg cttaacagca gacctttacc
     6241 tgcaaagcgg gaaaggacag ccgggatgtt catcattgag ttcagcggat cgcgtttcag
     6301 caaccaggca atacccagga acggcttgac gaacactttg tcatacagcc agtcaaatcc
     6361 ccaggcgttg taccaccagg tgcccagcag acggcccggc gcactgttgg cgatggaggt
     6421 caccagagta cgtttaccca gccacagcca ggctgccagc agaatgccga ccaccgcgac
     6481 cacgccagag gtaatttcca gggtcaacat gctgccgtgc gccagctccg tcgtttgcgg
     6541 aagcacgccc tgcagcggcg gtacaatcag tgcgccaacg aaggtggaaa ggatcagcag
     6601 cacaatcagc ggcaggctgt gagttacccc tttcacggcg tgagcgtgaa tttgttcttt
     6661 tccgtggaag acgatgaaaa tcatacggaa ggtgtagagc gaggtcataa acgcaccgac
     6721 cagacctgcc accatcagat tgatatgacc attcgccatc gcacccgcga ggatctcatc
     6781 cttactgaag aagcccgcag tgaccagcgg cagtgccgac agggctgcgc cgcccaccag
     6841 gaagcagaga taaaccagcg gaatagattt acgcagaccg cccatcttga agatgttctg
     6901 ttcgtgatgg caggccagaa tgacggaacc ggatgccagg aacagcagcg ctttaaagaa
     6961 cgcgtgagtc atcaagtgga aaatcgccgc atcccatgcc tgtacgccaa gcgcgaggaa
     7021 catgtagcca atctggctca tggtagagta agcgagaaca cgtttgatat cggtctgtac
     7081 cagcgcggca aaaccggcca gcagcagcgt aaccgcccca acaatgccca ccagatgcag
     7141 aacttccggc gtcatcagga acaggccgtg ggtacgggcg atcaggtaga cacccgcggt
     7201 taccatggtt gcggcgtgga tcagcgcgga gacaggcgtc gggcccgcca tcgcgtcggc
     7261 aagccatgtc tgcaacggca actgcgcaga tttaccgacc gcaccgccca gcagcatcag
     7321 cgtcgcccac atcagcatgt tattgccgtc agcaaagtgc gctagtgcca gttccaccat
     7381 ttcgcggaag ttcagggtgc ccagttcgtt gtaaagaatg aacagcgcga aagcgaggaa
     7441 cacgtcaccc acacgggtca cgacgaatgc cttcattgcc gctgcgccat tcttcggatc
     7501 ggtgtaatag aaaccgatca gcagatagga gcacaggccc
//
LOCUS       DAPOZO010000167         7131 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPOZO010000167 DAPOZO010000000
VERSION     DAPOZO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.168"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            82..1512
                     /locus_tag="ROI21_004522"
     CDS             82..1512
                     /locus_tag="ROI21_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HDX5113354.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQITFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMRSNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AKYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQKLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1553..2485)
                     /locus_tag="ROI21_004523"
     CDS             complement(1553..2485)
                     /locus_tag="ROI21_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDX5113355.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCECLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            2849..5635
                     /gene="polA"
                     /locus_tag="ROI21_004524"
     CDS             2849..5635
                     /gene="polA"
                     /locus_tag="ROI21_004524"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDX5113356.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(6017..6649)
                     /locus_tag="ROI21_004525"
     CDS             complement(6017..6649)
                     /locus_tag="ROI21_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDX5113357.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
BASE COUNT         2050 a         1609 c         1745 g         1727 t
ORIGIN      
        1 tgataatgcg tcctgccgct ggacattatt tcagcataag tggatattca aagttttgct
       61 gttttatcag ggaatattta tatgatacgt aagtcagcta caggtgttat tgttgcgtta
      121 gccgtaatct gggggggtgg cacatggtac acaggtacgc aaattcagcc tggtgtcgaa
      181 aagtttatta aagattttaa cgatgctaaa aagaaaggtg aacatgccta cgatatgacg
      241 ttaagttata aaaactttga caaaggcttt tttaattctc attttcaaat gcaaataaca
      301 tttgataacg gtgcacccga tctcaatatc aagccaggcc agaaagttgc atttgatgtg
      361 gatgttgagc acggtccgtt gcccatcaca atgttaatgc gtagtaatgt catccccgca
      421 ctggcagcgg caaaagtgaa cttagtgaat aatgaactga cacaaccgct atttatcgcc
      481 gcaaaaaata aatcgcccgt ggaagcgaca ttgcgattcg cgtttggtgg ctcattctcc
      541 acgacattag atgttgcccc tgcaaaatat ggaaagtttt cttttggtga gggccagttt
      601 acttttaatg gtgatggtag ttcattgtct aacctggata ttgaaggcaa agtcgaagat
      661 attgttctgc aattatcacc aatgaacaaa gtaacggcga aaagttttac cattgattct
      721 ctggcgcgat tagaagaaaa gaaatttccg gttggtgaaa gcgagtcgaa atttaatcag
      781 attaacatta tcaatcacgg ggaagacgtt gcccaaatcg atgctttcgt tgcaaaaacc
      841 aggctggatc gcgttaaaga caaagattat atcaatgtca atctgaccta cgaacttgat
      901 aagttaacaa aagggaatca gaaactcggt agtggtgagt ggtcattgat tgctgaatct
      961 attgatccct cagcggtgcg ccaatttatc atccagtata acattgcgat gcagaagcag
     1021 cttgctgcac accctgagtt agcaaacgat gaagttgctc tgcaagaagt gaatgctgca
     1081 ttgttcaaag agtatttacc gttattacaa aaaagtgagc cgaccattaa acaaccggta
     1141 agatggaaga acgcactcgg cgaactaaat gccaatctgg atatcagtat tgccgaccca
     1201 gccaaatcct catcatccac aaacaaagat attaaatcgc tcaattttga tgtgaagtta
     1261 ccgcttaatg tcgtcacaga aaccgcaaaa cagcttaatt tatctgaagg gatggatgct
     1321 gaaaaagcgc aaaagcaggc tgataaacaa atcagcggga tgatgacctt aggtcagatg
     1381 tttcagttaa tcacgattga caacaatacc gcctcgctgc aactgcgtta tacaccgggt
     1441 aaagttgttt ttaacggaca ggagatgagc gaagaagaat ttatgtctcg tgccggacgt
     1501 tttgttcatt aaaatactca ctcgcctctg ctcattcaga ggcgagcaaa atttacttgt
     1561 cctgccgctg tgatgacatc aacgacgtca gaagccggtc tttttcctgc cacagtgagt
     1621 tcagccattg ctgaaaatgg cgcttaaagc ttttgtcatt gatgtagtcg ccatgtaact
     1681 catcggcaat aggctgtaaa tccacatgta cgacaatccg cgtcagttta ccgcttaaca
     1741 tatcgaagaa cggctgacgg ttattgtccg gataacacag cgtaacatta agcagtttat
     1801 cgaattgttt acccagtaca tttagcgcca tcgcaatgcc tgccgccttt ggtggcaaca
     1861 agttttgaaa agtggagtgg gtttgctgat gtttttcttg cgtgaaacgg gagccttcaa
     1921 cgaaattgac aatggtggtg ggatgcaggc gaaacttttc acaagaacgc cgagtggttt
     1981 caacatcttt accacgtcgc tccggatgac gtaataaata agcgcgggaa taacgcttca
     2041 taaacggcat atccagcgcc cagcatgcca ggccaagaaa aggcacccag gccagctgct
     2101 gcttgaggaa atatttattc atcggaatgt gcttacgaaa cagcacgcac agtacgacaa
     2161 tatctgccca actacggtgg ttacaaataa gcagatacca gttcttctta cttaaccctt
     2221 ccagcccgtg aacttcccat tgcaggtgtg ggttcagatg cagtaatacc gcaaggcatt
     2281 cacaccagca atacatcata aaatcacaaa aacgcgaaac ctttcgccaa atgactggca
     2341 caggtagcaa tagttttaca atcccggcaa tgatgatcgg gacggaacaa aaaatagtga
     2401 ccaaaatggt caatacgata ctcagcaaaa gggttatcgc agcgagtatt ctcgtcataa
     2461 tgaatttatt caaaaggtta gccatagaca gtgcgctaaa gaagcgcaag gcgcagattt
     2521 tagcagaaaa cgacccaaat aacggatgat ccttaaggag aaaaataatt catatctatc
     2581 cacattagaa aaaatcccat tatcttaatt actagggatg gtcttatttt taactgcatg
     2641 aaaaacaaag acaaacatca tgctgtaaaa agcatgataa taaattaaaa gcgatgtaaa
     2701 taatttatgc acaaagttat ccacatgacg atttgcgagc gatccagaag atctacaaaa
     2761 gattttcacg aaaagcggtg aaaaactcat gttttcatcc tgtctgtggc atcctttacc
     2821 cataatctga taaacaggca cggacattat ggttcagatc ccccaaaatc cacttatcct
     2881 tgtagatggt tcatcttatc tttatcgcgc atatcacgcg tttcccccgc tgactaacag
     2941 cgcaggcgag ccgaccggtg cgatgtatgg tgtcctcaac atgctgcgca gtctgatcat
     3001 gcaatataaa ccgacgcatg cagcggtggt ctttgacgcc aagggaaaaa cctttcgtga
     3061 tgaactgttt gaacattaca aatcacatcg cccgccaatg ccggacgatc tgcgagcaca
     3121 aatcgaacct ctgcacgcga tggttaaagc gatgggactg ccgctgctgg cggtttctgg
     3181 cgtagaagcg gacgacgtta tcggtactct ggcgcgcgaa gccgaaaaag ccgggcgtcc
     3241 ggtgctgatc agcactggcg ataaagatat ggcgcagctg gtgacgccaa atattacgct
     3301 tatcaatacc atgacgaata ccatcctcgg accggaagag gtggtgaata agtacggcgt
     3361 gccgccagaa ctgatcatcg acttcctggc gctgatgggt gactcctccg ataacatccc
     3421 tggcgtaccg ggcgtcggtg aaaaaaccgc gcaggcattg ctgcaaggtc ttggcggcct
     3481 ggatacgctg tatgccgagc cagaaaaaat tgccgggttg agcttccgtg gcgcgaaaac
     3541 aatggcagcg aagctcgagc aaaacaaaga agttgcttat ctctcatacc agctggcgac
     3601 gattaaaacc gacgttgaac tggagctgac ctgtgaacaa ctggaagtgc agcaaccggc
     3661 agcggaagag ttgttggggc tgttcaaaaa gtatgagttc aaacgctgga ctgctgatgt
     3721 cgaagcgggc aaatggttac aggccaaagg ggcaaaacca gccgcgaagc cacaggaaac
     3781 cagtgttgca gacgaagcac cagaagtgac ggcaacggtg atttcttatg acaactacgt
     3841 caccatcctt gatgaagaaa cactgaaagc gtggattgcg aagctggaaa aagcgccggt
     3901 atttgcattt gataccgaaa ccgacagcct tgataacatc tctgctaacc tggtcgggct
     3961 ttcttttgct atcgagccag gcgtagcggc atatattccg gttgctcatg attatcttga
     4021 tgcgcccgat caaatctctc gcgagcgtgc actcgagttg ctaaaaccgc tgctggaaga
     4081 tgaaaaggcg ctgaaggttg ggcaaaacct gaaatacgat cgcggtattc tggcgaatta
     4141 cggcattgag ctgcgtggga ttgcgtttga taccatgctg gagtcctaca ttctcaatag
     4201 cgttgccggg cgtcacgata tggacagcct cgcggaacgt tggttgaagc acaaaaccat
     4261 cacttttgaa gagattgcgg gtaaaggcaa aaatcaactg acctttaacc agattgccct
     4321 cgaagaggcc ggacgttacg ccgccgaaga tgctgatgtc accttgcagt tgcatctgaa
     4381 aatgtggccg gatctgcaaa aacacaaagg gccgttgaac gtcttcgaga atatcgaaat
     4441 gccgctggtg ccagtgcttt cacgcattga acgtaacggt gtgaagatcg atccgaaagt
     4501 gctgcacaat cattctgaag agctcaccct tcgtctggct gagctggaaa agaaagcgca
     4561 tgaaattgca ggtgaggaat ttaacctttc ttccaccaag cagttacaaa ccattctgtt
     4621 tgaaaaacag ggcattaaac cgctgaagaa aacgccgggt ggcgcgccgt caacgtcgga
     4681 agaggtactg gaagaactgg cgctggacta tccgttgcca aaagtgattc tggagtatcg
     4741 tggtctggcg aagctgaaat cgacctacac cgacaagcta ccgctgatga tcaacccgaa
     4801 aaccgggcgt gtacatacct cttatcacca ggcagtaacc gcaacgggac gtttatcgtc
     4861 aaccgatcct aacctgcaaa acattccggt gcgtaacgaa gaaggtcgtc gtatccgcca
     4921 ggcgtttatt gcgccagagg attatgtgat tgtctcggcg gactactcgc agattgaact
     4981 gcgcattatg gcgcatctct cgcgtgacaa aggcttgctg accgcattcg cggaaggaaa
     5041 agatatccac cgggctacgg cggcagaagt gtttggtttg ccactggaaa ccgtaaccag
     5101 cgagcaacgc cgtagcgcga aagcgatcaa ctttggtctg atttatggca tgagtgcttt
     5161 cggtctggcg cggcaattga acattccacg taaagaagcg cagaagtaca tggaccttta
     5221 cttcgaacgc taccctggcg tgctggagta tatggaacgc acccgtgctc aggcgaaaga
     5281 gcagggctac gttgaaacgc tggacggacg ccgtctgtat ctgccggata tcaaatccag
     5341 caatggggct cgtcgtgcag cggctgaacg tgcagccatt aacgcgccaa tgcagggaac
     5401 cgccgccgac attatcaaac gggcgatgat tgccgttgat gcgtggctac aggctgagca
     5461 accgcgtgta cgtatgatca tgcaggtaca cgatgaactg gtatttgaag ttcataaaga
     5521 tgatgtcgat gccgtcgcga agcagattca tcaactgatg gaaaactgta cccgtctgga
     5581 tgtgccgttg ctggtggaag tggggagtgg cgaaaactgg gatcaggcgc actaagattc
     5641 gcctgaacat gccctttttt cgtaagtaag caacataagc tgtcacgttt tgtgatggct
     5701 attagaaatt cctatgcaac aactgaaaaa aaattacaaa aagtgctttc tgaactgaac
     5761 aaaaaagagt aaagttagtc gcgtagggta cagaggtaag atgttctatc tttcagacct
     5821 tttacttcac gtaatcggat ttggctgaat attttagccg ccccagtcag taatgactgg
     5881 ggcgtttttt attgggcgaa agaaaagatc cgtaatgcct gatacgccat gtttatcagg
     5941 ccaacggtaa aatcgtaatc tattgaattt acgggccgga tacgccacat ccggcacaag
     6001 cattaaggca agaaaatcat tcgccgtcct gcgtttcttc tacaggctgc atctcgctaa
     6061 accaggtatc cagtttctgc cgcagcttgt ccacgccttg tttcttcaac gaagaaaacg
     6121 tttcaacctg cacatcaccg ttaaacgcca gcaccgcttc acgcaccata ttcaattgcg
     6181 ctttacgtgc gccgcttgcc agtttgtccg ctttggtcag cagcaccagc acggcgatat
     6241 tgctgtctac cgcccactca atcatctgct gatccaaatc tttcagcgga tggcgaatat
     6301 ccattagcac caccagacct tgcaggctct gacgtttttc gaggtattcg ccgagcgcgc
     6361 gttgccattt gcgcttcatc tcttccggga cttccgcgta gccatagccg ggcaggtcaa
     6421 ccagacgctt gccgtcagcc acttcaaaca ggttgataag ctgggtgcgc cctggggttt
     6481 ttgaggtacg agccaggctt ttctggttag tcagcgtgtt cagcgcgctg gatttacctg
     6541 cgttggaacg gcctgcaaaa gccacttcaa ttccggtatc ggaaggtagg tggcgaatat
     6601 caggcgcact catcacaaaa tgcgtctgtt gataattcaa attagtcaaa gcggtcgtct
     6661 ccgtcagtca aagctttgcg gcgattatac ctgaaccata ataaaaggcg gatttttcgg
     6721 cgtgagcgtt gtaagtaaaa gccatacgct ttgtgagaca ttgccgatag tctttatgcg
     6781 aaatagcaga gaaaattctg cgatgcatgt caaataagct atataaatca gttaattgct
     6841 tttatataaa actctgaaaa tgtgcaaata ctgatggcgg ttgattgttt gtttaaagca
     6901 aaggcgtaaa gtagcaccca tagagcgagg acgctaacag gaacaatgac tcaggatgag
     6961 ggtcaggagc gccaggaggc gaagacagag gattgtcagg aagacaaacg tccggagacg
     7021 taattaaacg gaaatggaat caacacggat tgttccggct aatggaaaaa cagggtgtgt
     7081 tggcggcctg caaggattgt aagacccgtt aagggttatg agtcaggaaa a
//
LOCUS       DAPOZO010000168         6990 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPOZO010000168 DAPOZO010000000
VERSION     DAPOZO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.169"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            1..379
                     /locus_tag="ROI21_004526"
                     /pseudo
     CDS             1..379
                     /locus_tag="ROI21_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238190.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
     gene            711..1586
                     /locus_tag="ROI21_004527"
     CDS             711..1586
                     /locus_tag="ROI21_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HDX5113358.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATAQHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQILALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1594..2370
                     /locus_tag="ROI21_004528"
     CDS             1594..2370
                     /locus_tag="ROI21_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDX5113359.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTFS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2539..4800
                     /locus_tag="ROI21_004529"
     CDS             2539..4800
                     /locus_tag="ROI21_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDX5113360.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSLPEVIGNGW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTH
                     FGAGDITELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4869..6044
                     /gene="senB"
                     /locus_tag="ROI21_004530"
     CDS             4869..6044
                     /gene="senB"
                     /locus_tag="ROI21_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HDX5113361.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6567..6872)
                     /locus_tag="ROI21_004531"
                     /pseudo
     CDS             complement(6567..6872)
                     /locus_tag="ROI21_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT         1965 a         1656 c         1633 g         1736 t
ORIGIN      
        1 tggaatggca ggtgatggag tggcgagagg aagcctatac cctcgatgaa ctggctgatt
       61 atctcgattt gagcgcctca gcgcgccgta gcatcgacga aactgccacc tgttcgaaat
      121 gacgcgcaaa tgggcttaca gggcgattcg tcagggctgg cccgaattct cacagtggct
      181 tgatgccgtg attcagcgtg tcgaaatgta caacgcatcg cttcccgttc cgctttcacc
      241 gcctgaatgt cgggctattg gcaagagcat tgcgaaatac acgcacagga acttcacgcc
      301 ggaaactttc gcacagtatg tggctgatac gcacacatta acgtacgttt tcgttccatt
      361 ggccctcacc ccccgataac gacccaggta gcgcgccgta ttccccccgc cggccgtttt
      421 cttcgacatg tacatcaggc gcggaactca tcggggcgca ggaatgaaat cccattcaga
      481 ctccggatag attcaagcaa gcccacttat cgtcaaaatc ggtgttgcaa aaacgggagt
      541 gaccatagat ccgttttcca agcggaccca taatatcggc tggtaaacat cactcgctag
      601 tgcattcttt ttgttggtaa tgataatcat ttacattcat tctttgatgt tttatcataa
      661 ttaatgtttt gtcaataaat atatggaaac atattgtttc agatgggaat atgagaaagt
      721 tcatccttat ttctatgata acgttagcta gtgttagtct gggagcctgc agacagaatg
      781 tcaccataaa gcaggatgcg ccagggcaga aagctttcct gactgacggg cagatttacg
      841 atttgcacag tggcaaaatt atttctagca gcgagctttt ggcggatctt gctaccgctc
      901 aacatcttat tattggtgaa aaacacgata atgccgagca tcatcagatt gagctttggc
      961 ttattcaaaa tttgctcatc cagcgaccac aggggagtgt gttgcttgag atgttgacgt
     1021 cagagcaaca gccccgagtt aaccaagtta aatgttggct gaaagataat cctgtggttc
     1081 gagatagccg tgttcaggaa ctacttaact ggcaaaaagg gtggtcctgg gagatgtatg
     1141 gcgacatcgt catgcagcta ttacgtgggc cttacccgct gctcaatgcc aacattggcc
     1201 gggagcagat attggcactg tacaaaaaaa acgaatttcc taaaggaaaa aaatccactg
     1261 cacctgtggt acaagaagct ctgcgtgaaa ccatcatatc tatgcatgaa ggcaaccttg
     1321 aaagccagca actgacgtca atgctctcta ttcagcagca gcgggatcgc tacatggccc
     1381 gccagcttct gagtgccccg gtaccgtcgt tgttgattgc aggtggttat cacgcgtcaa
     1441 aaagcatggg cgtaccgtta cacatggaag atcttgccac cggcacccat ccagtggtgt
     1501 tgatgcttgc agaaaaagga atgaacatca cagtagatca tgctgactac gtgtggtttg
     1561 tagcgccaga tacaacaaaa aggtaaacca tggatgatga atattctcca cttctcacag
     1621 tcggtaaaat ggagcagctg gtttatctgt tctctactgc ttcatggact gattttcctt
     1681 gcgtttatct ggcgtttttc cgaagttcag cccgctatgt ctcctgcacc ggcgatcatg
     1741 ctgcaatggg cagaaattat agaggctcct tcttctccac tttcgcttcc ggtgggaatc
     1801 gctcaacagg agtccgcagt gacagaagaa aagcaacaaa ctgaggacag acaacaacgt
     1861 cctgtcactg aagacagtga tgcaaccatt gaaataacta ggaagaaaaa gtcatccgat
     1921 ggagaaaaga aaaagacacg tccacctcgt aagataaagg ctcaaaccag cgacagcaac
     1981 cccacagccg tttccagtaa tgctgcacct caggctctgg tcgaatcgtc ccgtattgct
     2041 gcgcctttta acagtgactc aacaaagcga gataattctg aggcatcttg ggaaagcaga
     2101 gtaaaaggcc acctaaaccg ttataaacgt taccctggcg atgccagaaa acgtgcacga
     2161 accggcacag ccgtcgtgac ttttaccgta aacacggaag gcactattgt cagtagtttc
     2221 ttggaaatct cttcaggaac cttttctctt gaccgcgaag ctattgctgt tttagaacgc
     2281 gctcaaccac ttcccaaacc accgcctgaa atattggagg gaggattatt taaagtaaaa
     2341 atgccgatta cctttaagtt gaaggagtaa agggataatt ggccgttcaa gttcatttaa
     2401 ctgaaattta attttcattt ttatgctgaa cattttatca aacttttcca ctgtcaaaaa
     2461 aatcaaaaag acagtagaac ataattaata aacaaatagt tatacttatc tttattaata
     2521 ttatacccaa ggaaatttat gaacgttata aaactggcta tcggctcagg catattattg
     2581 ctcagctgcg gtgcttactc acaatccatc agtgaaaaaa ctaattccga caaaaaagga
     2641 gcggcagaat tcagtccgct cagcgtttct gtcgggaaga cgaccagtga gcaggaagct
     2701 ctcgagaaaa caggcgcgac cagttcccgg acaacggaca aaaacctgca atcacttgac
     2761 gcaacagtgc gtagtatgcc tggtacttat actcaaatag atcctggtca gggagcaatc
     2821 agtgtgaata ttcgaggcat gagcggattt ggtcgtgtaa acactatggt cgatggtatt
     2881 acccagagtt tttacggaac ctctacctcc ggaacaacga cgcatggttc aactaacaat
     2941 atggctggcg tacttataga tcctaactta ctggtagcag ttgatgttac acgcggtgac
     3001 agcagtggct ctgaagggat caacgccctt gccggtagtg caaatatgcg tactattggc
     3061 gttgacgatg taatatttaa cggtaataca tatggccttc gttcacgttt ctctgtcggt
     3121 agtaatgggc tgggacgcag cggaatgatc gcccttggtg gaaaaagcga cgcttttacg
     3181 gatacgggaa gcattggcgt tatggctgct gtgagcggca gttctgtgta ctctaatttc
     3241 tcaaatggtt ctggaattaa cagcaaagag tttggttatg ataaatatat gaagcagaac
     3301 cccaaatccc aactgtataa aatggatatc agaccagacg aatttaacag cttcgaactt
     3361 tccgctcgaa cctatgaaaa taaatttaca cgtcgtgata taaccagtga cgactattac
     3421 attaaatatc attacacccc tttttctgaa ttaattgact ttaacgtaac ggccagtacc
     3481 agtcgcggta atcaaaagta tcgtgatggc tcgctgtata ctttctacaa aacctcagcg
     3541 caaaatcgtt ctgacgcgct ggatatcaac aataccagcc ggttcactgt cgcggacaat
     3601 gacctggagt ttatgctggg cagcaaactg atgcgtaccc gctatgaccg gaccattcac
     3661 tcagcggcgg gcgacccgaa agcgaatcag gaatcgatcg agaacaatcc gttcgcaccc
     3721 tccggccagc aggatatttc agcgctgtat accgggctga aggttacgcg cggcatctgg
     3781 gaggcagatt tcaatctcaa ctacacacgt aacaggatca cagggtacaa gcccgcctgc
     3841 gattcacgcg ttatctgcgt gccacagggt agctacgata ttgacgataa agagggtggc
     3901 ttcaaccctt cagttcagct ttctgctcag gtaacaccat ggcttcagcc gttcattggc
     3961 tacagcaaat ccatgcgcgc cccgaacatc caggagatgt tcttctctaa ttcaggaggc
     4021 gcatccatga acccattcct gaagcctgaa cgtgcagaaa cctggcaggc gggttttaac
     4081 attgatacca gagatttact ggtcgaacag gatgccctgc gctttaaggc tctggcgtac
     4141 cgcagcagga tccagaacta catctacagc gagtcttatc tggtttgttc tggaggtcgt
     4201 aaatgcagtc tgcctgaggt gattggcaat ggctgggagg gcattagcga tgaatacagc
     4261 gacaatatgt acatctacgt taactcggca agcgacgtta tcgcaaaggg cttcgaactg
     4321 gagatggatt atgatgcagg ttttgctttt ggccgactct ctttcagcca gcagcaaaca
     4381 gaccagccaa cctccatcgc cagcacccac tttggcgcag gggatataac cgaactgccc
     4441 agaaaataca tgacgctgga tactggtgtt cgcttcttcg ataacgcgtt gaccctgggc
     4501 actatcataa aatacacagg caaggctcgt cgcctgtcgc ctgattttga gcaggacgaa
     4561 cataccggcg caataatcaa acaggatttg ccgcagatcc caacgattat cgatctctat
     4621 ggtacttacg agtacaaccg caacctgaca ctgaaacttt cggtacaaaa cctgatgaac
     4681 agagattatt cggaggcgct gaataagctc aacatgatgc caggtcttgg tgacgagacc
     4741 cacccagcca attccgcgcg tggcagaaca tggatatttg gcggggacat tcgtttctga
     4801 tgacaatttc cgctaggttc agacaatacg ttttctctct tatgtcaatt ttattgcagg
     4861 aacgaaaaat gaatattttc actttatcca aagcaccgct atacctgtta atttcactat
     4921 ttttacccac gatggccatg gctatcgatc cacctgaacg cgaactttcg cgatttgccc
     4981 tgaaaacgaa ttaccttcag tcccctgatg aaggcgtcta tgaactggcg tttgataatg
     5041 ccagtaaaaa ggtgtttgca gcagtcactg atcgtgtaaa tcgtgaagcc aataaaggct
     5101 atctgtattc gtttaattca gattcgctga aagtcgaaaa taaatacacg atgccatacc
     5161 gggcattttc gctggcgata aatcaggata aacatcagct ctatatcgga cacacccagt
     5221 cagcgtccct gcgtatcagt atgtttgaca ccccaaccgg caaactggta agaaccagcg
     5281 acaggttaag ttttaaagcg gcaaacgctg cagattcgcg ttttgagcat tttcgccata
     5341 tggtttacag ccaggattcc gataccctgt ttgtgagtta tagcaatatg ctgaaaacgg
     5401 ccgagggcat gaagcctctg cataagctgt taatgctcga cgggacgacg cttgccttaa
     5461 aaggcgaggt taaggatgct tacaaaggta cagcgtatgg tctgacgatg gatgaaaaaa
     5521 cacagaaaat ctacgttggc ggaagagatt acatcaacga aattgatgcg aaaaatcaga
     5581 cgctgctgcg taccatcccg ttgaaagatc cgagaccaca aatcacaagt gtgcagaatc
     5641 tggcggtgga ctccgcttct gaccgtgcct ttgtggtggt attcgaccat gacgatcgtt
     5701 ccggtacaaa agatggactc tatatttttg acttacgcga cggtaaacag cttggctatg
     5761 tgcacacagg agccggagct aacgcggtga aatacaatcc gaaatataac gaactgtatg
     5821 tcaccaactt cactagcggc accatcagcg tagtggatgc caccaaatac agcatcaccc
     5881 gtgaatttaa catgccggtc tacccaaacc agatggtgtt gtcggacgat atggataccc
     5941 tttacattgg catcaaagaa ggctttaacc gcgattggga tcctgatgtg tttgtggaag
     6001 gagctaaaga acgtattctg agcattgatt tgaaaaagtc gtgatcgggg ggaaaaaaca
     6061 tgggataaaa actccgttat tccactaaac tggccggact aggaaaagca aacatctctg
     6121 tcctgccggt acgcctgtta cgccgctcca tgaggctgag gaatctccag aaaacgagac
     6181 aggcacagca ttgtacgatc aactgatttg tgccaataat taaatgaaat cacagctatg
     6241 cctgttcatg gggttctagg ccggaacccc agatttttcc gattgtttgt tttctaactg
     6301 attattcagt agttttagag ggctgacgct cagtggaacg aaaactcacg ttaagaggtt
     6361 ttgactatga taaaagtcaa agtaggggtg agtttggata tggaaattat tgtactataa
     6421 ggagagcatg aaaaaccctg gggcagtcac tgaatgaggg accagcagcg gtaaaataac
     6481 cagcataatt tctgcatggt cgatttttat accgtgacag gctgtttttt atacttgcac
     6541 cgattcccgg gcacacttag cctgtgtcag gccggcatat acctcagcag actgatatcc
     6601 cgcgcgtttt tcttcagccc tgatacattc agccccgcaa tggcccggcg gtataccatc
     6661 cggccgccgc acagcacgca ttcgaacggg tcacgactca ggaactgttt caccatttgt
     6721 gcatagcaca ctttcgccac tgcttccggt ttatccatcc ccagagcacg gtacacctgc
     6781 ggcagcttct ctccacacac ccggttggca aggaacccga agtacctcac catcttaaaa
     6841 aactgaaccg ccccgggaat cctggagact aaactccctg agaaagaggt aaacaggatg
     6901 actaaaaata ctcgtttttc ccccgaagtc cgtcaacggg cagtccgtat ggttctggaa
     6961 agtcagggcg aatatgactc acaatgggcg
//
LOCUS       DAPOZO010000169         6972 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPOZO010000169 DAPOZO010000000
VERSION     DAPOZO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.170"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..758
                     /locus_tag="ROI21_004532"
     CDS             <1..758
                     /locus_tag="ROI21_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDX5113362.1"
                     /translation="GIMGKSGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLNGHG
                     HSMVITDLPGVGESRDRDAEYEALYRDILLELDLVLWLIKADDRALSVDEYFWRHILH
                     RGHQRVLFVVMQADKTEPCHEWDMAGIQPSPAEAQNIREKTEAVFRLFRPVHRVVAVS
                     ARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTA
                     ESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            976..3372
                     /locus_tag="ROI21_004533"
     CDS             976..3372
                     /locus_tag="ROI21_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDX5113363.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR
                     EALELMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTS
                     DLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCA
                     FREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML
                     "
     gene            3369..4274
                     /locus_tag="ROI21_004534"
     CDS             3369..4274
                     /locus_tag="ROI21_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HDX5113364.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQSETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            4271..5341
                     /locus_tag="ROI21_004535"
     CDS             4271..5341
                     /locus_tag="ROI21_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDX5113365.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            5477..6160
                     /locus_tag="ROI21_004536"
     CDS             5477..6160
                     /locus_tag="ROI21_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113366.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            6176..6586
                     /locus_tag="ROI21_004537"
     CDS             6176..6586
                     /locus_tag="ROI21_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113367.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            6807..>6972
                     /locus_tag="ROI21_004538"
     CDS             6807..>6972
                     /locus_tag="ROI21_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDX5113368.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLE"
BASE COUNT         1802 a         1685 c         1902 g         1583 t
ORIGIN      
        1 ctggcattat gggtaaaagc ggggccggta aatcctcact ctgtaatgcg ctgtttcagg
       61 gggaggtcac cccggtcagt gatgttcacg ccggcacccg ggaagtgcag cgcttccgtc
      121 tgaatggcca tggtcacagc atggttatca ctgacctgcc cggggtgggc gagagccggg
      181 acagggatgc agagtatgaa gccctgtacc gtgacattct gctcgaactg gacctggtac
      241 tgtggctgat taaagccgat gaccgtgccc tgtctgtgga tgagtatttc tggcgacaca
      301 tcctgcaccg gggacatcag cgggtactgt ttgtggtgat gcaggccgat aaaacggagc
      361 cctgccatga atgggatatg gccggtattc agccttctcc cgcagaggca cagaacattc
      421 gcgaaaaaac ggaggcggta ttccgtctgt tccggcccgt acatcgggtt gttgccgtat
      481 cggcccgcac cggctgggaa ctggatacgc tggtcagtgc gctcatgaca gcacttcccg
      541 accatgccgc cagtcccctg atgacccgcc tgcaggacga gctgcgcacg gagtctgtcc
      601 ggtctcaggc ccgtgaacag tttaccggtg cggtggaccg gatatttgac acggcggaga
      661 gcgtctgcat tgcctctgtt gcacgcacgg ttctgcgcgc cgtccgtgac tcggtggtct
      721 ctgttgcccg cgcggtatgg aactggattt tcttctgaac ccggtggtga tgtaccaggc
      781 tgttgttaca gatttttaac tccatgaaaa cactatgcaa acgcagccat acccgggttg
      841 tccccttgcg ttgtgtttct caagttaata gaatacatcg atcgttacta tcatatcgat
      901 cgttataaac gatcgtttgt agtaaatgac ctcctccttg gttgtcatga aaatgcgtct
      961 taggaaggtt aaataatgca cgaaaagaac atcgccctgc tttgtgatga agccgaccga
     1021 cttttgcaac tgaacattaa tctgctccgg caaatggttg aggagccaga tgtgttatct
     1081 gacagtaaga acgaaaacag actgcttttt gataaacaga aagcactgaa aagaattgag
     1141 gagctggagg gcgaacaaat caaaaccgcc cgcagggaga tggtgctggc tgttgtcggc
     1201 acgatgaaag caggcaaatc aaccaccatc aacgccattg tggggcagga aattctgcct
     1261 aaccgtaacc gccccatgac ctctgtaccg acgctcatcc gccacgttcc cggaaaaact
     1321 gagccggttc tccatctgga acatattcag cctgtccgca atttattaat cacactgcag
     1381 gaaaaactcg ccaccccggc aggacagcag gtcgcacaga ccctgcagca aaccggggat
     1441 acccgcgaac tgctggatat tctgacggat gatggctggc tcaaaaatga ataccacggg
     1501 gaggaggaaa tctttaccgg actggcatcg ttaaacgatc tggttcgtct tgctgcggca
     1561 atggggactg aatttccttt tgatgaatac gccgaagtgc agaaactgcc ggtgatcgac
     1621 gtggaattca gccatctggt ggggatggat gcatgccagg gaacactcac actgctggat
     1681 acccccggcc ctaatgaggc cggacaaccg cagatggaag tgatgatgcg ggatcaactg
     1741 cagaaagcct ctgcggttct ggctgtgatg gattacaccc agatgaactc aaaagcggat
     1801 gaagacgtcc gtaaagagct taatgccatt gctgacgtat cagccggccg cctgtttgta
     1861 ctggtcaata aatttgatga gaaagaccgc aatggcgatg gggcagatgc cgtacgccag
     1921 aaagttccgg caatgctgaa cagcgatgtg ctgcccgcct cccgtgttta tcccggatcc
     1981 tcacgccagg catacctggc taaccgtgcg cttcatgagt tacggaaaaa cggaaccctt
     2041 cctgttgatg aagcctgggt cgatgatttt atcagaggtg ccttcggttg catgaaaaaa
     2101 gaatacgtct gtaaagacag tgaactggca actgaagggg cgacagacct gtgggaaggc
     2161 tcacttatcg atcaactgat aacggaagtc atacagagct cacattccag agctgcggca
     2221 ctggcggttg actctgccgc cgcaaaactg atgcagaatg cagaaaatat cagtgaatac
     2281 ctgttgttac gccatcaggg gctacagcaa agcattcagt cactgcagtc gcatatcacc
     2341 agcctgcttg cggatatccg ggaaatcgcg gactgtcagg agcagatgac cactgatgtc
     2401 agaatggcca tggaggagat cgatactaaa acccgggaat tactgacggg ggtctgcacc
     2461 tcactggaag aggagctgaa tgactatttc agaagcggta aacgcaaaga acagcaaatg
     2521 ctggaggaag aagacgcaga acaacgaagg tctcagtccg gattatgggg gaaaatttct
     2581 caatggtccg gtatcaacaa ccagggccgg gaggattaca ggaaacgaga ttttgccccg
     2641 gacagccccg aaataaaatt cagtgatcgc agggaagccc ttgaactgat gacgcaaatc
     2701 gaatcgaccg tgaccagcct gcaccgtgag gctgaagcac agttccggcc tgagctggag
     2761 aaaatcgtca gcgggattga aacaggtttt cgtggcacgg ccctgtacgc cacagaaaac
     2821 attgccggtc gcatcaatgc ccgcctggag gatgagggct ttaccgtaaa aatcagtttt
     2881 ccggcagtca gccagttaca gacccggctc gcggtaaaaa taaatctgag tgcgcttatg
     2941 gaggaaagaa cggagaccgt cacccgtcgc cgtcggcaga gtggcgtatg gggaaccgtt
     3001 tgtcgatggt ttggcaccag tgacctgggc tgggaaaact atgacgagga tgtgagtcgc
     3061 agcgtgatca atatcaacaa ggtcagagag gaagttatgt cactgacccg ggcatatttc
     3121 ggggagctgc aggcatccat tgagcaggat attaaccagc ccgtccgcca ggaaatcgat
     3181 gcctttttct gcgcattcag ggagaaagtt gaacaactgc gtaacacgct gattcagagc
     3241 tctgaagatc ataaacgcga tcagcaggcg caggaacggc ttaccgggcg acttcaggca
     3301 ttaaatgaaa gggttcctga gctcattact gacagtaagg cgctgaggga agagctggag
     3361 acaatgctgt gacttcacca tttattcaac aaattgctga taaccgggta tgtcaggtac
     3421 tcacctgtct tcctgaaaaa tttgtggttg atttcgcaaa cggaatcgat gttgcacagg
     3481 agcatatccg cacggccgga gagcgcacgt tcttccggcg tttaaaagag gggctgaccg
     3541 gcgaaggcgc tgcacggcag aatgccatca atgcgtcgct tgcacagggc gttgaggcgt
     3601 ccctgcgctg gctgacggag atgacaacgt cacttgccac cacaaactac gcgattaccc
     3661 gggtaaacga cagggtcagt tcactggtca gtgatacagc caggctggcg cattattccg
     3721 cagatacgcg ggagcagtta ctcaccctgg ccgatcaggt tcaccataaa ctgaatcatc
     3781 ttgaagaaaa actccaccgt gttgaccagg ttcagcgggc gcagttacat cttgagcaga
     3841 tattctcatg gtggagcgcc gggcgatatg cgtcattttc ccctgccgga cgttgttatg
     3901 tggcacttga agagcttcgc tggggcgcgt ttggtgatgt gatacgtcag agcgaaacag
     3961 gccaggttaa ccagctactg gatattctca gacataaagc attaacgcag atggcacagg
     4021 agagtggcgg tagtgcaacc gtgcgtctga acactctgga ctggcttggt ggtcagggcc
     4081 gggagcaggc agataacgag tggcatgacg cgattaactg gctgggagac tggtgcagtg
     4141 aagagcagca tccggtgatc tggtcaacca cgcaggctgc agaacactta ccggttcgca
     4201 tgcctcgcct ctgctctgca gaacgcctct ctgaaagtat ggtcgatgaa atatttcaga
     4261 agggggctgc atgagtacag aaatgaaaac ggggctggtg ctgtccggtg gcggggcggt
     4321 gggcgcttat caggcgggag tggttaaggc actggcagag tgtggtacac agatcagcat
     4381 ggtttcaggg gccagcattg gcgcattcaa tggtgccatt atcgcggcct ctcccgatct
     4441 gtcagaagct gccgtacgcc tggaggcgct ctgggatcat ctggggaata atcaggtgct
     4501 gtcggtaaac agattggttt acttttcatt gctgaaaaaa ttgttccagg caatgaacct
     4561 ctgccagatc cccggacgtg caggagcact gcttacgacg cttcttcgcc atatatcgac
     4621 aatcaacggg tttgacaatc tgatggctca gccgttgttg tcagatgagc ccctgacagc
     4681 gctgatggat cattatcttg atactgatgc tctggcagac gggctaccgc tgtatgtgtc
     4741 gctgtacccc acagaagggg gcatgcagga tattattgac tgcattcgtg ctgaactggg
     4801 tgtcggaacc acgaaaaacg ccgtttttca gcatatccag agcctgcccc gcggacagca
     4861 gaaagaggct ctgcttgcgt cagccgcgct gcccctgctg ttccgtcccc gtgaggttca
     4921 ggggacaatg ttcggtgatg gtggtatggg aggatggcga aatatgcagg gaaatacccc
     4981 tgtgacgcct ctggtcgatg ccggatgcaa tatggtgatt gtgacgcatc tgagtgacgg
     5041 ttctttatgg gatcgccagg cttttccgga caccacaatc cttgagatcc gtccccggaa
     5101 aaggctgaaa tatgcaggtg atggtggcaa cagcggcggt ctgctcagtt ttacatcggc
     5161 acataccgac gcctggcgtc agcagggcta tgaagacacg atgctggcga tggagcatat
     5221 ccggaaaccg ctggcagcac gtcaggcact gacccggtca gaggcggtat tgcagaaaag
     5281 cctggatata acggaagagg cagatttggc actgagaaac gcgatggccc ggattaaata
     5341 aagacgcccc aaagaaacgc caccgcctgt ggtggtgttt ttcctgtctg gtgttattga
     5401 aaatattttc agatatatat tacctgagag aaagttttac ccctcacctg tcaattcact
     5461 cactggaaat ttaattatga aattacctgt aaagttatta atgagcctta tatctctggt
     5521 cagcgttatt gcacgtgccg gggaatataa aaattacagc cgggatgaaa taaaatactg
     5581 gcgatataca tcatacaagg gggggaaact tccggaaggt ttcactgatg agaaattttc
     5641 cagcgccatt tacaacggaa gaatatttac aatgaaacgt ttacataccc ttatgttatt
     5701 tctggcggtt ctgttcacgg gctttaactt taacgcagaa gcggctaccg taaaacaagc
     5761 tcttagttgt aacccagaag cctgggctga acaacctgga gcgtgtccat caacgtacga
     5821 gttgtacgaa ggtgacgcta cctacaaagc cgctatcgac aaagcattaa aaccggtcgg
     5881 actgagcggc atgttcggta aaggcggcta tatggatggc cctggtggag gtatcacgcc
     5941 agtaaacatt aacggtacag tctggttcca gggcgacggt tgcaaagcca atacctgcgg
     6001 ctgggacttt atcgtaacac tctataaccc aaaaacccat gaagttgttg gctaccgtta
     6061 ctttggttta gatgacccgg cctacctggt ttggttcggt gaaattggcg tgcatgaatt
     6121 cgcgtatctg gtgaaaaact acgtagctgc ggttaactaa tttaaggagt ctatcatgaa
     6181 aacttttatc aaaactttac tcgttgctgt aactattctg ttctctgtct tcgctactgc
     6241 gaaacaagta aaactgccaa ataacatcaa atacgttaat actacagaag cgttttcctg
     6301 taccgaaatt gacggtatga attgccagac aaagaatcag ttcaactata aagataacag
     6361 ctatgttttc gtgcttgaac gtggtggtgc ctggtgctac gactacactg tctcggtagt
     6421 taacctgaaa accgggaaag cacaaatgat tgaatacggg gacaatcaac tgtgctcagg
     6481 tagcaacaaa ccgttcttcg aaatcaaaaa tggcgtaccg acggtaggag tcatcgacac
     6541 atccggaaaa cctgtcgttg tagcccagga caaacttaaa atctaaggag taaaccatgc
     6601 gtttctctgc acattattaa tatcaaaggt aaatacagcc ccagccattg ttaccggaaa
     6661 gccgcttaat gcgtggctcg ccagtgaaag gcctctcaga caataaacct tattcatttc
     6721 acccgtcagg ccgcgtcttc tccgggagac gcggcttttt tcatttattg caccagtaaa
     6781 tcttaaccac cgataaggag caaagtatgc gattagcaag tcgttttggt tatgctgcaa
     6841 accagatacg ccgtgaccgt ccgctgacac atgaagaact gatacgccat gtacccagta
     6901 tttttgggga agaccggcac acctcccgca gtgaacggta tgcgtacatt cccaccatta
     6961 ccgtcctgga aa
//
LOCUS       DAPOZO010000170         6695 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPOZO010000170 DAPOZO010000000
VERSION     DAPOZO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.171"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(52..1728)
                     /locus_tag="ROI21_004539"
     CDS             complement(52..1728)
                     /locus_tag="ROI21_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDX5113369.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(1966..3186)
                     /gene="fsr"
                     /locus_tag="ROI21_004540"
     CDS             complement(1966..3186)
                     /gene="fsr"
                     /locus_tag="ROI21_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDX5113370.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            3404..5056
                     /gene="ushA"
                     /locus_tag="ROI21_004541"
     CDS             3404..5056
                     /gene="ushA"
                     /locus_tag="ROI21_004541"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDX5113371.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKGGKLDDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(5093..5572)
                     /gene="ybaK"
                     /locus_tag="ROI21_004542"
     CDS             complement(5093..5572)
                     /gene="ybaK"
                     /locus_tag="ROI21_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDX5113372.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(5776..6570)
                     /locus_tag="ROI21_004543"
     CDS             complement(5776..6570)
                     /locus_tag="ROI21_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDX5113373.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLCAMPPGRYVVAVGALHLYGEGNLPQMLR"
BASE COUNT         1774 a         1725 c         1728 g         1468 t
ORIGIN      
        1 gcgcgtctta tcaggcctac atatccgcgc ctattatccg gcaaccaccg tttaccccgt
       61 caccacctca cccgccggtg gcgtttcaag cagttccagc atagtacggg cgatttcacg
      121 ctcacccatc actacctgat ttgccccacg ttcggtgata tacgccactt catcgtcata
      181 atgggcgcgg gcaataatct caatatccgg atttttcgcg cgggcagatg ccacaatctc
      241 gcccgcttca taaccgttgg gaatcgtcag gatcagccat ttagcacatt ccagatgcgc
      301 cagttgcata atttcttcgt tcgccgcatt gcccaatact gcgcggaccc cgcgctctcg
      361 cagttcatca acacgggttc gtgacgtctc aatcaccact aaaggaatat cagaggcgag
      421 caatttctcc cccagcaggc tgcctacacg accgtaacct accagtagcg catggttgca
      481 aatatccact gggatctgct tctcttcttc gatagcctct tccagcgtct gctcttccag
      541 cgtttcggtc ttcgccagat atttctccag cagcgcaaac aggaccgggt tgagcataat
      601 cgacaaaatc gcccctgcca gtaccaggtt ctgcccggct tgcggcaaca gatccagcgc
      661 catgcccagt cccgccagga taaacgcgaa ctcaccaatt tgcgccaggc tggcggcgat
      721 agttaatgcc gtacgttggg agtgaccaaa cagtcgcacc aggaaaaacg cggctaacga
      781 cttaccaaac agaataatcg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat
      841 taacggatca aacaacatcc cgacggagac aaaaaacagc accgcaaacg cgtcgcgcaa
      901 tggcagcgta tcgtgggcgg cacggtgact cagttcagac tcgttcagta ccatcccggc
      961 aaagaacgca ccgagtgcaa aggagacatc aaacagctcc accgcaccaa aggcaatccc
     1021 taacgccagc gccagcaccg acagggtaaa cagctcacgg gaaccggttg ctgcgctgcg
     1081 tgccataatc cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat
     1141 caccttgccg atggtgatcc ccatatcgac agcaagagtg gcaaagccca catcgccttg
     1201 ttccatcatt cctgccactg cgggcagcaa caccagcgtc agaaccatta ccaggtcttc
     1261 cacaatcaac caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc
     1321 aagtgcgcgc agtaacacca cggtactggc ggtggaaagg cataagccga acacgatacc
     1381 ggtcattaac gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc
     1441 tatctgggcg atcgcaccgg gaatggcgat ggcctttacc gccatcaaat ccttgagcga
     1501 aaagtgcaga cccacgccaa acatcaacag aatgacgccc agttcagcca gttccggcgc
     1561 aagcttggta tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag
     1621 atatcccacc agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag
     1681 cacaaggccg ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt
     1741 tcctggtggt tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg
     1801 acaatttcat tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc
     1861 tgcaaaaaaa gcacggatcg gaaaacagaa ggcgatttaa cagaggaaag gcttatggcg
     1921 actgtaaaac gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg
     1981 gttatcaggc aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata
     2041 gactaactcg atgctggtgt ggtcggcgat aagccccaga actgccgctc ccagacctcc
     2101 catgccgaaa gcaaaaccga aaaagagtcc agaaaccata ccgatacgtc ccggaagcag
     2161 ctcctgagcg tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac
     2221 tgttaaaacc cccgtccagt gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac
     2281 gccgaggata gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc
     2341 gatcaccgta ccagccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg
     2401 gatagataat ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat
     2461 atagaaatat ttcgagaaaa tgaggattaa cagaatgctg accgccagta ccactttgtt
     2521 gcgcggcagt ggattgataa tcgtcgcttt tggttttcct ttattcattc ggtgctgtgc
     2581 cgagtaccaa cggctgattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa
     2641 ccaggctacg ttgcctttgc cataaggcgc gataattacc gctgccagca agggccccag
     2701 ggaactgcca aagttgccgc cgacctgaaa gatagattgc gccagaccat gccgcccgcc
     2761 ggaagccata cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac
     2821 cagcgccgcc gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc
     2881 acttaaggta aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca
     2941 gtaaccgacc accggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc
     3001 aatctgcata aatgtcagag aaaactctga ctgaagcagc ggataaatcg ccagaatcag
     3061 cgattggatc atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga
     3121 tgttcgggcc ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat
     3181 tgccatagga aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta
     3241 ttacccgact aacatacctg tatgcgtcgc ctgaaggaag tctcaacgct gaatacagaa
     3301 tttctaatct ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc
     3361 atactatttt tcaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg
     3421 gggcgtggcg ttagcactgt taaccacatt tacactggcg agtgaagctg ctctggcgta
     3481 tgagcaggat aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt
     3541 ttggcgcaat gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat
     3601 ccgcaaagag gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa
     3661 cactggcgtg cccgagtctg acttacagga tgccgaacct gattttcgcg gcatgaatct
     3721 ggtgggctac gatgcgatgg caatcggtaa tcatgaattt gataacccgc tcaccgtatt
     3781 acgccagcag gaaaagtggg caaagttccc gttactttct gccaatatct accagaaaag
     3841 taccggcgag cgcctgttta aaccatgggc gctgtttaag cgtcaggatc tgaaaattgc
     3901 cgttattggc ctgacgacgg atgacacagc gaagttaggc aacccagaaa acttcaccga
     3961 tattgagttc cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac
     4021 agaaaagcca gacattatta tcgcggcgac ccatatgggg cattacgaca atggtgatca
     4081 cggctctaac gcaccgggcg atgtggagat ggcgcgcgcg ctgcctgccg gatcgctggc
     4141 gatgatcgtc ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca
     4201 ggtcgattac gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt
     4261 gcaggcgcat gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga
     4321 aatgaaaatg gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga
     4381 agacgggaaa agcgagcgcg tgctgtacac ccctgaaatc gctgaaaacc agcaaatgat
     4441 ctcgctgtta tcgccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcga
     4501 aaccaatggt cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg
     4561 gcggttaatt ctggcagcac aaatggatcg cactggtgcc gactttgcgg tgatgagtgg
     4621 aggcggaatt cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt
     4681 gcagccattc ggcaatgtgg tggtgtatgc cgacatgacc gggaaagagg tgattgatta
     4741 cctgaccgcc gttgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgctaacgt
     4801 tagctttgtg gcgaaaggcg gcaaactgga cgaccttaaa atcaaaggcg aaccggtcga
     4861 tccggcgaaa acttatcgta tggcgacatt aaacttcaat gccaccggcg gtgatggcta
     4921 tccgcgcctt gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct
     4981 gaaagcgtat atccagaaga gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga
     5041 ggtgagctgg cagtaatccg aaagtgccgg atatttgcat ccggcacaat gcttaatcgc
     5101 ggcgggcgat atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt
     5161 cgatatccag cccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg
     5221 cgtcgataat cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccagat
     5281 atcccgtcga acgctgcgca accatcggat cggccatctc aactttcttg gcacccagcg
     5341 cttttgctac ttttttaaga tccagttgac ctgcaaccgg cgtaacggcc acggcaaggt
     5401 gtttcatatc accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac
     5461 ctaatttttt gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat
     5521 ggatttgaaa cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt
     5581 tcctgcctta aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag
     5641 tcatcttgcc taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt
     5701 cgcttaaagt gacggcataa taataaaaat atgaaattcc tctttgacgg gccaatagcg
     5761 atattggcca tttttttagc gcaacatctg cggcaaattc ccttctccat acaggtgtag
     5821 tgcaccgact gcgaccacat agcgccccgg cggcatggcg cataatttgt cccgccaggc
     5881 gagatttcgc tgatgcatca gcacatcgta cagcgattga ctgaacgtat taggcagcgt
     5941 tatttcatta ttttgcggcg gcgcattcag ccaccagctc atcatttgtt gcagcaaccg
     6001 tgcattggtg tgccagtggg tcagcgtatc gtccagcagt gccagtccgt tgtcagggag
     6061 ctggagcaac atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg
     6121 ttgcttcgcc gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt
     6181 ttgtgcctgc gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag
     6241 tgagggagaa atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct
     6301 aatgcgctct tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga
     6361 aacatccgcc tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg
     6421 caggggagcc atatcgtggc tgcccatatg aatactgcca atcagatgaa aatggcgatt
     6481 gccgggaaga gagatatcga tagcaggcca ggtgtaatga ttaccgcgca gcgcggccca
     6541 aagcgttttt acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag
     6601 cgcgcgggga gggatggcgc aaatggagtt atgaggcttg ttcagtggta gcttaaatat
     6661 gatcgaaaaa ttgtttttgc tttgaaacga aagac
//
LOCUS       DAPOZO010000171         6358 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPOZO010000171 DAPOZO010000000
VERSION     DAPOZO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.172"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(92..514)
                     /locus_tag="ROI21_004544"
     CDS             complement(92..514)
                     /locus_tag="ROI21_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDX5113374.1"
                     /translation="MAKVFTPEEREKIKGQVVELVRLSGRETLRALEAKTGASRYYIS
                     TLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEVMQRVLAFYQGNFQDVLL"
     gene            complement(530..1291)
                     /locus_tag="ROI21_004545"
     CDS             complement(530..1291)
                     /locus_tag="ROI21_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HDX5113375.1"
                     /translation="METVFDVLKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPAPAELPEETTTATVAKISESDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLLRVNQGGKFRYCIPGDNLPAEPKAASVAETAGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQITDSSRHVASEK"
     gene            complement(1314..2060)
                     /locus_tag="ROI21_004546"
     CDS             complement(1314..2060)
                     /locus_tag="ROI21_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDX5113376.1"
                     /translation="MKNIATGDVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNHSGIQPLHRKCSFSNYQVQNEGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIAK"
     gene            complement(2067..2855)
                     /locus_tag="ROI21_004547"
     CDS             complement(2067..2855)
                     /locus_tag="ROI21_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113377.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNAECNARGVTKSAIDRITFMAGFADALIQVGWLVETNGVLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTKTDQKALPEEEKEEDINTYLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFSPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            complement(2933..3355)
                     /locus_tag="ROI21_004548"
     CDS             complement(2933..3355)
                     /locus_tag="ROI21_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113378.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETNAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQDALSTAIDAHDDAVQAVYRKAHFSGGGSPSDSVVVH"
     gene            complement(3352..3606)
                     /locus_tag="ROI21_004549"
     CDS             complement(3352..3606)
                     /locus_tag="ROI21_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDX5113379.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            3686..4105
                     /locus_tag="ROI21_004550"
     CDS             3686..4105
                     /locus_tag="ROI21_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5113380.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQIRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            4348..4527
                     /locus_tag="ROI21_004551"
     CDS             4348..4527
                     /locus_tag="ROI21_004551"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113381.1"
                     /translation="MQQLVPPPGVKGIDKMVHYEVVQYLMDCCGITYNQAVQALRSND
                     WDLWQAEVAIRSNKM"
     gene            4538..4693
                     /locus_tag="ROI21_004552"
     CDS             4538..4693
                     /locus_tag="ROI21_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaF family protein"
                     /protein_id="HDX5113382.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LEKHTVS"
     gene            complement(4690..5178)
                     /locus_tag="ROI21_004553"
     CDS             complement(4690..5178)
                     /locus_tag="ROI21_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein B"
                     /protein_id="HDX5113383.1"
                     /translation="MLDVFTPLLKLFANEPLERLMYTIIIFGLTLWLIPKEFTVAFNA
                     YTEIPWLFQIIVFAFSFVVAISFSRLRAHIQKHYSLLPEQRVLLRLSEKEIAVFKDFL
                     KTGNLIITSPCRNPVMKKLERKGIIQHQSDSANCSYYLVTEKYSHFMKLFWNSRSRRF
                     NR"
     gene            5620..5841
                     /gene="kilR"
                     /locus_tag="ROI21_004554"
     CDS             5620..5841
                     /gene="kilR"
                     /locus_tag="ROI21_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HDX5113384.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            5841..6011
                     /locus_tag="ROI21_004555"
     CDS             5841..6011
                     /locus_tag="ROI21_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113385.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            6086..>6358
                     /locus_tag="ROI21_004556"
     CDS             6086..>6358
                     /locus_tag="ROI21_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113386.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPTEKKAEFLATLIAMKLKGEI"
BASE COUNT         1704 a         1526 c         1399 g         1729 t
ORIGIN      
        1 taaattttat ttacagtgct aaatttaatt attcagattt ggattatgat ttctctttca
       61 gttcacgcag ttccctgatt gttaatttgg ctcacaacag cacatcctga aaattaccct
      121 gatagaacgc cagtacacgc tgcatgactt cgctcttccg gcactcgcga cagattatat
      181 tcagacgcct gtcatagcgg cgtatttctc cgtctggtaa tgaccagata aggtccggat
      241 caactacagc aggtttcttc acctttgccc tcgatagttt tttgcgggcg ttttgccagt
      301 ctttacgcgc ctgctcagac gggaataatc cgtagcctga attgtaaaca tcaccactgg
      361 cgaccagttc tctggcgaga gtgcttatgt aataccttga tgcaccggtt ttagcctcca
      421 gagcccgtaa cgtctcgcga ccgctcagac gtacaagttc aacaacctgc cctttaattt
      481 tttcccgctc ttctggtgta aatacttttg ccataggtgc ctccggcaat cacttttccg
      541 atgcaacatg gcgggaagaa tcagtaatct gtcgtacaat atccctgtgc ttgttcagct
      601 cccgcagcgc ggcgcagaca cgctcccact tctggacatg atttttcgcc cgacgcagtt
      661 cgcggtttgc catatgcagt gatggtaaaa ccaggtcatc cgctcgcgtt tcagtaaacg
      721 atggcagcga ctgcacaatg tccgccacag tttctgtttt aatatcttcc tgtgttgcag
      781 cctcctgtac tggtaacgca acacctgcgg gctgaggaaa ggccttacca gcagtttccg
      841 ctaccgatgc tgctttcggc tctgctggta aattatcgcc cggtatgcag taacgaaatt
      901 taccgccctg atttacgcga agcagacgac ctttgctgat tgccattgcc agcgttgaag
      961 ccactttgcg tgatgtggta ccaaacaatg tagccagctc atccgccgtt tgtggtccac
     1021 gttgttcaat cgtcgcagtt aaatcgctct ctgaaatttt cgcgactgtt gccgtggtag
     1081 tttcttccgg cagttctgcc ggcgctggct gttcctgctg aacgttgtta tcagccacac
     1141 gccaggtgta cgcgctttta tcaacgaaac cagccttttt cagttcccat agttcgttca
     1201 gcacttcttc acgactgata tcaagtcgcg cagcaagttc tatggatgtg gcttttccca
     1261 ttgctttcag tacgtcaaaa acagtctcca ttaaattttt ctcccggtaa aaattacttc
     1321 gcaattcctg gctggacgac attcggacgc cagctctccc agttgaaatt cacccatcgc
     1381 ccgccgttca tggtcatgcg atccataatc cgctcgccaa gcaatgtttt catggcctca
     1441 tagttcaggt ttgtcagcat ccccacgctg cgcatcgacg ctgtccggcg atcaacaatc
     1501 tggtgcagta ccacctgctc gtttttcgtc tcgcgctgaa tgccaatttc atcaagaacc
     1561 agcagatcca cttcgcacag ttcccgcaaa aatttttcgc ctgactgccc gtcgtcatag
     1621 ctggcgtgca gggcactcat aacatcagcc acggtaacca caatcactgt ctgaccgtct
     1681 ttcagcaggc gattcccgat agctgccgct aagtggttct tcccggtacc aggttttccg
     1741 ctgaacgcaa aatttgtaca cccggtcatc agttcatcag cgatggattt cgcctgactc
     1801 aacgcgtatc gctgcccttc gttctgcacc tggtaattcg aaaacgagca tttgcggtgc
     1861 aatggctgga tgccagagtg attcagaatt ttttccaccc gcaactgacg attctgacgg
     1921 ttgatctcct cacaacgttt ctggccttcg gaaagttgcc actcgcgcca ctccgctacc
     1981 gtcttgaatg gcgcggttac atgtgacggg gccagtctgc ggatacgttc aagaacgtcg
     2041 cctgtcgcaa tatttttcat ggtcagttac cccctgaagc ctggcgggat cgcactatcc
     2101 ggtaacgaga cggtgttaac ctgtcggagt aacgtctcag gtcgaacacc tttcggcgcg
     2161 aacaagccct ggtattcgtt ggcgatgctg tgtcgaatca cctgctcagg tgaaaaaccc
     2221 tgctggcgga atttttccag ctcccgtatc gccccgttag cgccctgctc cgttcgaatc
     2281 ggttttcgca atgcctgcct gaactgaacc cactcatgcc agagtgtttc cggcaaccag
     2341 tcaggcagct cgatagcctc cggttcgaat tttttagacg ctcgtttttg gcgaggggga
     2401 tttaggggga gataagtatt tatatcttcc tctttctctt cctctggtaa cgctttttga
     2461 tccgtttttg taacgctggc agcgttacct tttcgtttca gttctcgtat ttttgttact
     2521 ctctcgtttg taaccgcccg ttttttagag cttttcccgt tatggcgctc aaagttagga
     2581 agcgacaaca caccattagt ttcgaccagc catccaacct gaattaacgc atcagcaaaa
     2641 ccagccataa aagtgatgcg atctattgca ctttttgtaa cgccgcgagc gttacactct
     2701 gcgttaccgt ctatcatttg ttgatccgcc catgcccaga agcgaatgac tttccctaat
     2761 gcggcatctg gatcaatatt cagaatctca gcaagcctga atatttccgg cttatccggc
     2821 gtaataactt cgagctttat ccagtttgaa gccatttgtt ttcaccttgt aacgctcgca
     2881 gcgttacatt taactgatac cgaacaaaac agttcggtac gattaatttc aatcaatgca
     2941 ctacgacaga atcgctagga gaaccgccgc cgctgaaatg tgctttacgg taaacggcct
     3001 ggactgcatc atcatgcgca tcaattgccg tactcagtgc atcctgtgcc gccagtaatg
     3061 cacggcgttc cagggtatcg aagatgcaga gtcggtgacg cagctcgcga ggaagaattg
     3121 ccagaaccgc agggatcagt ttctgaattt tttccctttg cgcattcgtt tcacctttca
     3181 accaacgatg atagatattc tgctgattgt tccagtcctt gcctggtacc aggggcaatt
     3241 cgccgccccc ctggcgcaga tattcttcag taattgcgtt agcgacccac gcctgccctt
     3301 tttcggctgc cagggctaac aacactgatt cgatgtgctc atgcttgatt ttcatgaatc
     3361 aactcccatc agctttttcg tagtagtttt atttctgcca atagttaaaa ttgcatcggc
     3421 agaaaataat ccgtttgatg catgagcgat tttttcagcg taatttgttt cgccggtata
     3481 ttctgtgcga ggcaattttc cgttatccat ccatttgtag attgctcttt ggctgacacc
     3541 acaaacgtcg gccacaacag aaacgcgaac agttttgatt acatcttcaa gtgttttctg
     3601 gttcatatca ccctcacaat gtgaactttg agtacatgct ataacagaac tgacagtaca
     3661 ttcaagagcg aatatcattg aacttatggt tcatgaagat aaagcgcgta aagagttcgc
     3721 cagtaggctt gcgctagcct gtgaaaacgc tggttatgaa caacatggaa ggcaggcaga
     3781 aattgcccgt cgaatgaaat taacaccaaa agcggttagc aaatggttta atggtgaaac
     3841 aattcctcgc cgagagaaat taagggaatt agcaacactc attggaacaa caccaaccta
     3901 tcttttggga gaggatacag aagaaagtgg acagatacgt ttctatcagg agttaaatcc
     3961 aagacaaaaa atcatcattg accttctgga cgagctccct gacagtgaga cagatgaact
     4021 tttaaaaact cttgaggaga aaaaacagaa gtacaatgca atttacgaag agttagcacg
     4081 aaagaaaaaa caaaaagcct cttaaaccag cataaatccg gtagcgtccc cctccgggtt
     4141 tgtgcttcac tttatcccgt ctcatttttt tatacataaa atgtacttaa agtactttac
     4201 aatgatgaac acaaagtaca ttatatacct accaacccac cccgccccac agaacgccgg
     4261 gcaatacttc gagttaccag gcagtggtca ggggttaagt agccagcccg aggcgtatga
     4321 acatgacggc gggattcaaa ttttgcagtg cagcagttag ttccgccacc cggcgttaag
     4381 gggatagata agatggtgca ttacgaagta gttcagtatt tgatggattg ttgcggtatc
     4441 acttacaacc aggctgtgca ggctttacgc agcaacgact gggatctctg gcaggcagaa
     4501 gtcgctatac gtagcaacaa gatgtgagat tcgcaaaatg caaaaaatcg acctcggcaa
     4561 caacgaatcc ctggtgtgcg gcgtgttccc caaccaggat ggaacgttca ctgccatgac
     4621 gtataccaaa agcaaaacat ttaaaaccga aactggtgcg cgccgatggt tggagaagca
     4681 cacagtaagc taacgattaa aacgtctact cctgctgttc cagaataact tcataaaatg
     4741 ggagtatttt tcggtgacga gataataaga acagtttgcg ctatcactct gatgttgaat
     4801 gatgcccttc cgttctaatt ttttcataac cgggttacgg caaggagaag tgataataag
     4861 atttcctgtt ttaaggaaat ctttaaatac agcgatttct ttctcagata aacgaagcaa
     4921 tactcgttgc tctggtagta atgaataatg cttttgaata tgtgctcgca atcttgagaa
     4981 ggaaatggcg accacgaaag aaaaggcaaa aacgataatc tgaaagagcc aaggtatttc
     5041 agtataagca ttgaatgcga cagtaaactc tttcggtatc agccagagag tgagaccaaa
     5101 aatgataatc gtatacataa gtctttcgag tggctcgtta gcaaaaagtt tcaacaatgg
     5161 agtaaataca tccaacatat caataactct caactgtaag ggtattgaaa tgttaacaca
     5221 agctctcgct gtaggggtat agccgagacc accgaagccc ggaggtggtg aaataaaacc
     5281 gggcacaaca cgaaggcgca tttccgatat ccataaagag tcggtcttgt ctgttaaatt
     5341 taaatggtgg gagtgcgcct ccggttgtaa ataacgacat tgctgtgtgt agtcctggcg
     5401 gcatcagttt ttttcttgaa gttcggctga tgtccgccct ttttaaagtg aattttgtga
     5461 tgcggtgaat gcggctaagc gcacgtggca cagttaaaag tcatgttagt ccttattggt
     5521 ttgggtggga aagccgactg taattgttaa ctggttgcag tcacctggag gcaccaggca
     5581 ccgcatcaac aaagttcatt tgtaaaaatg gagataatta tgattgcaca tcacttcgga
     5641 actgatgaaa taccacgtca gtgtgtgact cctggcgatt atgttcttca tgaaggccgg
     5701 acatatattg cctcggcaaa caatattaaa aagcgaaaac tatatattcg taacctgacc
     5761 acaaaaacat gcattactga ccgcatgatt aaagtcttcc tcggtcgtga tggtttacct
     5821 gtaaaggcgg agtcatggtg atgactaaga aaataaaatg tgcttaccac ctttgcaaaa
     5881 aagacgttga agaaagcaaa gctattgaaa gaatgcttca cttcatgcac gggattttat
     5941 caaaagacga accgagaaaa tattgcagtg aagcttgtgc cgaaaaagac cagatggcac
     6001 atgaacttta attaattgac tattcgaaac tgaatttatg ccagaaatgg caggtattcg
     6061 ctcaacctta attaaggaga aaaacatgat taccaattat gaagccactg ttgtaactac
     6121 cgatgacatt gttcacgagg tgaatctgga aggaaagcgc attggctacg taattaaaac
     6181 agaaaataaa gaaaccccat tcactgtggt tgatatcgat ggtccatcag gcaacgtaaa
     6241 aacacttgat gaaggtgtca aaaaaatgtg cctggtgcat atcggaaaga atctgcccac
     6301 agaaaaaaaa gccgaatttc tggcaactct aattgcaatg aaattaaaag gtgaaatc
//
LOCUS       DAPOZO010000172         6262 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPOZO010000172 DAPOZO010000000
VERSION     DAPOZO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.173"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            29..700
                     /locus_tag="ROI21_004557"
     CDS             29..700
                     /locus_tag="ROI21_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDX5113387.1"
                     /translation="MSEDNPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQ
                     DPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIA
                     KQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDPQYPGMET
                     KTASDYLPQTTWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTS
                     RGIRK"
     gene            697..1266
                     /locus_tag="ROI21_004558"
     CDS             697..1266
                     /locus_tag="ROI21_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113388.1"
                     /translation="MKRALFFLLMIFVSFGVIANCETQAKDQDCFTIFTKGTIFSAFP
                     VLNNKTMWRWYQNEDIGEYYWQTELGICKNNKFTPSGARLLIRVGSLRLNENHATKGT
                     LQELLNTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKDESYECLTKVQHELLRSEEK"
     gene            1537..1782
                     /locus_tag="ROI21_004559"
     CDS             1537..1782
                     /locus_tag="ROI21_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113389.1"
                     /translation="MALELSYVYIKYIYGKEKAEFQKPYSITDDNNCWKIEGKQPKTL
                     GGNFTILIAKKDGQALDVIHKIIHCSDDSNILPRCVS"
     gene            2365..2649
                     /locus_tag="ROI21_004560"
     CDS             2365..2649
                     /locus_tag="ROI21_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113390.1"
                     /translation="MNLISRIAYGTTEWNFYNNEDLENGYLVFTDVTFFNIIPNGSIP
                     DDYIVSIITDKVNVECFESTIVAVGQIRNTNVGDIDNIGECQILIRYKDR"
     gene            2934..3299
                     /locus_tag="ROI21_004561"
     CDS             2934..3299
                     /locus_tag="ROI21_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDX5113391.1"
                     /translation="MIRIAFLITNKFLEIDSTTLAGYDFSGMNLHRATLNGYCLDGAK
                     FEKTHLRNVMIQHTSTRNAVFHNAALMNAILNNSDFTGSDCSNSRSIGENFKAIDNHG
                     KDIINGKGLSNVCKIHDNV"
     gene            3271..3647
                     /locus_tag="ROI21_004562"
                     /pseudo
     CDS             3271..3647
                     /locus_tag="ROI21_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3752..4594
                     /locus_tag="ROI21_004563"
     CDS             3752..4594
                     /locus_tag="ROI21_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDX5113392.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCKDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            4622..5091
                     /locus_tag="ROI21_004564"
                     /pseudo
     CDS             4622..5091
                     /locus_tag="ROI21_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         2105 a         1074 c         1353 g         1730 t
ORIGIN      
        1 cgcagaatac gatgaatggg gtaaccagct gagtgaggat aacccgcacc agctgcagca
       61 gctcatccgc ctgccgggtc agcagtatga tgaggagtca ggcctgtatt acaaccgcca
      121 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      181 gggatggaat ttttatcagt atccgttaaa tccggttcag tatatagatt caatgggact
      241 ggcatctaaa tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc
      301 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      361 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagtcctta
      421 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta cgccgcagga
      481 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaac
      541 ttggccaaca actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga
      601 cattaataaa cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc
      661 taaaatgaaa cagagaacat cgagaggaat aagaaaatga aaagagcttt gttctttttg
      721 ctgatgatat tcgttagttt tggtgttata gctaattgcg aaacacaagc taaagatcaa
      781 gattgcttta ccattttcac taagggtact attttttccg cgtttcctgt cttaaataat
      841 aaaaccatgt ggagatggta tcagaatgag gacataggtg agtattattg gcaaacagaa
      901 cttggtatat gtaaaaataa caaattcaca ccaagcgggg cgagactctt aattcgcgtc
      961 ggatcactac gccttaatga aaatcatgca accaaaggaa cgttgcagga gttgctaaat
     1021 acagcagaaa aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc
     1081 tatcaaaaaa agagcagtga tccagctcag ttgttggcag tccttgataa ttcaatcatg
     1141 gttaaatatt ttaaagatga gaagccaact tatgctcgaa tgacagcaca tctcccgaac
     1201 aaagatgagt cttacgaatg tttaacaaaa gtacagcatg aactgcttcg tagtgaagaa
     1261 aaataaaatc tcttcctgag aaaataaatt aactattcga aaaaatatgt tcttaatggg
     1321 tatccaggaa caccagaagg tcgatttgga aaaatggatt ccgatggtaa gaatatcata
     1381 tattctgaat gtaatgtttt acccacaact tttgagattc gataggtgta gagcatgaga
     1441 aactcactaa tattacttat tatgatattg tttacaatat cacactcagt atttggaaaa
     1501 gaaaattcag tggaaatttt agtaaatagt cgtgaaatgg ctttggaatt atcatatgtt
     1561 tatattaaat atatatacgg gaaagaaaaa gcagaatttc agaaaccata tagtattaca
     1621 gatgacaata attgttggaa aatcgaggga aaacaaccga agacgttagg aggaaatttt
     1681 acaatactaa tagcgaaaaa agatgggcaa gcgttggatg taatacacaa aataatccac
     1741 tgttcagacg attcaaatat tttaccaagg tgtgtttcat aactattttg tgtacaatat
     1801 gactctacat atcgacgaaa aaaccacttg atttgacagg aacacattca aaaaaatttc
     1861 tttttcattt caggagcgat tttcatctgt tacttgaagt atctgtgaga ttaagtgact
     1921 ccccctaacg tcgtactaaa tagtaattac tggccatacc gggagcagta ttcggcgctt
     1981 cccacatgca gcagaataac cgcatcagcg aagatgtatt gtatttttac tatcacaatg
     2041 tgtacggacg gtttatggaa aagaatgaac attagatacg cgatgcggcg gcagattaca
     2101 cggttatcac tacgatagcc aatactggct ggtgcattac cgccgtgaac aataggacgc
     2161 cgtactgctg gaaatttctt acctttatga ctccccgcac cgccgtatcg ggaaacggga
     2221 agcagtcaag atgaggctga tataatgtgt ggaatgcctg agtattttta gaatagaggg
     2281 taatatgttt gatagaatat atcttggtga tcgaccaata aaaaaattga atttgattta
     2341 tggagcaaac aaattagaat tcaggtgaat ttaatctcaa ggatagccta tggtacaaca
     2401 gaatggaatt tttataataa tgaagactta gaaaatggtt atttagtatt taccgatgtt
     2461 actttcttta atataatacc taatggctcc attccagatg attatatagt ttcaataata
     2521 acagataaag tcaatgttga atgttttgaa tcaactatag ttgctgttgg tcaaattcga
     2581 aacacaaatg ttggtgacat tgataatatt ggtgaatgtc aaattctcat caggtataaa
     2641 gaccgctaga ttgagaataa actcaaagaa aaaataattg aataaccatg tcagttatag
     2701 acattttaga tggaacgaac cagagaaggt catgcctccg agctgggtta tgtggacacg
     2761 gagctttagc gtagtgaact caacgaagca agccggaaac ctgccagccc ttatcagcac
     2821 gtaagggaca acagcgtagt acgcagaata atcctaccca cgtagtatgg acacaggcct
     2881 aagcgaggtt ctggttttca aatttttcag gagttagatg attggaggaa cagatgataa
     2941 gaatcgcatt tttaataacc aataaatttc ttgagataga tagtaccact cttgctggat
     3001 atgattttag cgggatgaat ctacatcgtg ccaccctcaa tggttattgt cttgatgggg
     3061 cgaaatttga aaaaactcat ttaagaaatg taatgattca acatacatca actagaaatg
     3121 ctgtctttca caatgcggct ctgatgaatg ctattctgaa taactcagat ttcactggaa
     3181 gtgattgtag taattcaagg tcaataggtg aaaattttaa agcaatagat aatcatggga
     3241 aagatataat aaatggaaaa ggattgtcta atgtttgcaa aatacatgac aacgtataag
     3301 aaaatattat tcagaaccta tgtgattgta tctttgtttt tacatacaac acatgggcgc
     3361 agtgtaataa tattattata atgcgcaagt atgaatgtga ggggaaatac accgttagaa
     3421 atttggtaaa aaataaagct atagcattgg aattagctga gatgtatgtt aaaaatcgtt
     3481 atgggcatga tgccgcagaa gaagaaaaac catacaaaat catggaatta ccaacaagtt
     3541 ggattgttga aggcaccatt gaatcagacc aaattgctgg tagggttttt attatagaaa
     3601 taggcaaaaa tgacgggaga attctaaatt ttggccacgg aaaataagta tgtaaggata
     3661 aacaaacatc gtgtgtgctg atggcagagt aggtggagga ctccagacag tcaaacgata
     3721 gaaaaagata gtctttatgg aggttcctgc aatgtcaaat acataccaga aaagaaaggc
     3781 aagtaaagag tatggtttat ataatcaatg taagaaacta aatgatgatg aattatttcg
     3841 cttacttgat gatcacaatt ccttgaaaag gatttcatct gccagagtat tacagttaag
     3901 aggtggtcaa gacgctgtta gattggcaat tgagttctgc tctgataaaa attatatccg
     3961 tagagatatc ggagcattta tactcgggca aataaaaatt tgcaaaaaat gcaaagataa
     4021 tgtgtttaat attttgaaca atatggcatt gaatgataag agcgcttgcg ttcgagctac
     4081 ggcaatcgag tcaacggctc agcgatgcaa gaaaaaccca atttattcac ctaaaatagt
     4141 agaacaatct caaattactg cttttgataa atcgactaat gtcagacgtg ctacagcatt
     4201 tgctatttct gttatcaatg ataaagcaac aattccacta ttgattaatc tgttaaaaga
     4261 tccaaatgga gatgtcagaa actgggccgc atttgcaata aatatcaata aatatgataa
     4321 tagtgatatt agggattgtt ttgtggagat gcttcaggat aaaaatgagg aagtccgtat
     4381 tgaagcaata atcggacttt cctacagaaa agataaaagg gttttatctg ttttatgcga
     4441 tgagttaaaa aaaaatactg tttatgatga tatcattgaa gctgcgggtg aattaggtga
     4501 taaaacgcta cttcctgttt tagatactat gttgtacaag tttgatgaca atgaaattat
     4561 aacttccgct attgataagc tgaagcgttc atagcgtgag ttgcctatgc acagtggggg
     4621 attcccgccg gcacggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
     4681 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgaa gcagatgcaa
     4741 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
     4801 cgcggcctgc cgctggtgct tatcagcacg gaaggggcaa cagaatggtg cgcagaatac
     4861 gatgaatggg gcaacctgct gaatgaagag aacccgcaac agctgcagca actcatccgc
     4921 ctgccgggtc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
     4981 gatccgctgc aggggaggta tatcactcag gattcgattg ggctgaaggg gggatggaat
     5041 ttttatgagt atccgttgaa tccggttcag tatatagacc cttttggatt agctgatatg
     5101 aatttctatg atgaggggat acgcagaaat gtatcactgc atgctgcaac agataaaata
     5161 aatattcctg gcgtttttac tattggagga catggatatc ctggttttat tacagatgaa
     5221 aatggaaact atgaaactgc tgagggagta gccaagagaa tcagagctaa tccacgctat
     5281 aaaaagggaa tgtctgtttg attattctca tgtggtacag ccagagaggg agataaatct
     5341 tttgccagca agttagccag agcacttcat gcaaatgtca ctggagctga tacacaatgg
     5401 caagtatgga tggcgtggga taataatact cctcttttta atacgatcca aacaggactt
     5461 acggataata tctcggcaat tgacacaagt aataatgtca taactggtaa taatatgggc
     5521 cgctggataa cgtttggacc ttcaggtcaa cccatttctt ccactcctgg caaagttatg
     5581 agaccaagtg atataaaata acaaaggtgg caacatgaag tgctttttat ttattctgat
     5641 tgcaaatgtt ttctttttct cttgttgttc atatggaggt gaaatccccc ccctattaag
     5701 ctccataaca ttgaaataag ctcaacatct gaatcgttta atttaagtga aaatcaaaga
     5761 tatgaacttg atattaaatc tgagggcggg gatggtgatg cgtcatatcg attacatcaa
     5821 tattatacat atgtttgctt taatataccc aagcaaatta aatatctggc aaggtctgca
     5881 tctcaaggaa atatcatagc gcaatataat tatgctatga ttctatcctc agaggaggat
     5941 gattcctatt taaaatatta taatttagat aatgcaatta attggctgaa aatggctgcc
     6001 tcacatggct atattaaagc cgaggttgaa ttgctaaggc ttgaaaggct aaaagaaact
     6061 ataaggttag ggaaagatca tccaatgccg taggcacaaa gatgcaaggc aagatatttt
     6121 tcatattata agcataatgt tgtgaaaagc ggggctgcga ttgtattaaa gccatttttc
     6181 tggcttctga aggctgaaat tcagtcatga ttactcaggc tctgcgcctc catcagacca
     6241 ccgttgatca ccatatccat gt
//
LOCUS       DAPOZO010000173         6243 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPOZO010000173 DAPOZO010000000
VERSION     DAPOZO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.174"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..593)
                     /gene="grpE"
                     /locus_tag="ROI21_004565"
     CDS             complement(<1..593)
                     /gene="grpE"
                     /locus_tag="ROI21_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDX5113393.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            716..1594
                     /gene="nadK"
                     /locus_tag="ROI21_004566"
     CDS             716..1594
                     /gene="nadK"
                     /locus_tag="ROI21_004566"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDX5113394.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLYTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            1680..3341
                     /gene="recN"
                     /locus_tag="ROI21_004567"
     CDS             1680..3341
                     /gene="recN"
                     /locus_tag="ROI21_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDX5113395.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETAHALHLQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            3490..3831
                     /gene="bamE"
                     /locus_tag="ROI21_004568"
     CDS             3490..3831
                     /gene="bamE"
                     /locus_tag="ROI21_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDX5113396.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(3893..4183)
                     /locus_tag="ROI21_004569"
     CDS             complement(3893..4183)
                     /locus_tag="ROI21_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDX5113397.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(4173..4610)
                     /gene="ratA"
                     /locus_tag="ROI21_004570"
     CDS             complement(4173..4610)
                     /gene="ratA"
                     /locus_tag="ROI21_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDX5113398.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            4781..5263
                     /gene="smpB"
                     /locus_tag="ROI21_004571"
     CDS             4781..5263
                     /gene="smpB"
                     /locus_tag="ROI21_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDX5113399.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALALYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            5478..5840
                     /gene="ssrA"
                     /locus_tag="ROI21_004572"
     tmRNA           5478..5840
                     /gene="ssrA"
                     /locus_tag="ROI21_004572"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(6001..>6243)
                     /locus_tag="ROI21_004573"
     CDS             complement(6001..>6243)
                     /locus_tag="ROI21_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDX5113400.1"
                     /translation="MEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGI
                     KASFSSTLSGHLGVGYSHGAGVESPWNAVAGANWSF"
BASE COUNT         1642 a         1653 c         1468 g         1480 t
ORIGIN      
        1 tacgcttttg ctttcgctac agttaccatc gccgcacgaa tcgtacgacc attcagcgta
       61 taacccttct gcataatgcc cagtacgtta cctggcgcaa cgtcatcaga ttccaccatt
      121 gcgatagcct gatgcacatt cgggtccagc ggcacgttag tttcggcgat cacttcgaca
      181 ccaaacttac gcacaacatc cagcatcgac ttcagcgtca gctcaatgcc ttcaaccatc
      241 gcagacatat ccgggttagc tttatcagcc acttccagcg cacgatccag gctatcaatc
      301 accggcagca attcgttgat gaatttctcc agcgcgaatt tgtgggcttt ttcaatatcc
      361 agttcagtac gacgacgcag gttttccatt tcggctttta cacgcaaaat gccgtcacgt
      421 tcacgggtct gggcttcagc cagctgagct tcgagattcg caattttttc atcgcgcgga
      481 tccacctgct cagcagaagc ttctggctca actgcctcaa tctcttcgtg ctgatccatg
      541 ataatttctt ccggggcttg cccctcaggc gttttctgtt ctttactact catgaatttc
      601 tccgcgtttt tttcgcattc atctcgctaa cttcgcttat tatggggatc agtttcaggg
      661 tttcaaggga agcactcaca ttgtcatcaa tcttcgcaac aaggacctcg gaaaaatgaa
      721 taatcatttc aagtgtattg gcattgtggg acacccacgg caccccactg cactgacaac
      781 acatgaaatg ctctaccgct ggctgtacac aaaaggttac gaggtcatcg ttgagcagca
      841 aatcgctcac gaactgcaac tgaagaatgt gaaaactggc acgctcgcgg agattgggca
      901 actggctgat ctcgcggtag tcgttggtgg cgacggtaat atgctgggcg cggcacgcac
      961 actcgcccgt tacgatatta aagttattgg aatcaaccgt ggcaacctgg gtttcctgac
     1021 cgaccttgat cccgataacg cccagcaaca attagccgat gtgctggaag gccactacat
     1081 cagcgagaaa cgttttttgc tggaagcgca agtttgtcag caagattgcc agaaacgcat
     1141 cagcacggcg attaacgaag tggtacttca tcctggcaaa gtggcgcata tgattgagtt
     1201 cgaagtgtat atcgacgaga tctttgcgtt ttctcagcgt tctgatggcc tgattatttc
     1261 gacgccaaca ggctccaccg cctattccct ctctgcgggc ggtcctattc tgacaccctc
     1321 tctggatgcg attaccctgg tgcccatgtt cccgcatacg ttgtcagcgc gaccgctggt
     1381 gataaacagc agcagcacga tccgtctgcg tttttcgcat cgccgtaacg acctggaaat
     1441 cagttgcgac agccagatag cactgccgat tcaggaaggt gaagatgtcc tgattcgtcg
     1501 ctgtgattac catctgaatc tgattcatcc gaaagattac agttatttca atacattaag
     1561 caccaagctc ggctggtcaa aaaaattatt ctaattttac gccagcctct ttactgtata
     1621 taaaaccagt ttatactgta cacaataaca gtaatggttt ttcatacagg aaaacaacta
     1681 tgttggcaca actgaccatc agcaactttg ctatcgttcg tgagcttgag attgattttc
     1741 atagcggcat gaccgtaata actggcgaga ccggcgcggg taaatctatt gcaatagatg
     1801 ccctcggcct ttgtctcggt ggtcgcgctg aagccgacat ggtgcgtacc ggcgctgctc
     1861 gtgccgacct gtgcgcccgt ttttctctga aagatacgcc agcggccctg cgctggctgg
     1921 aagaaaacca gcttgaagac ggtcatgaat gtttgcttcg tcgcgtaatc agtagcgatg
     1981 gtcgctcacg aggttttatc aacggtacag ctgttcctct gtcacaactt cgcgaactgg
     2041 gccagttgtt gattcagatc catggtcagc acgcccatca gttactcacc aaacccgagc
     2101 accaaaaatt cctgcttgat ggctatgcca atgaaacctc tctgctccag gaaatgaccg
     2161 cacgttatca gttgtggcat caaagctgcc gtgacctcgc acatcatcaa cagttaagtc
     2221 aggaacgcgc cgcccgtgcg gaactgctgc aataccaatt aaaagaactt aacgaattta
     2281 atccgcagcc cggagagttt gagcaaatcg acgaagagta caaacgtctg gcgaacagcg
     2341 gtcaattgct gactaccagc cagaatgcat tggcagtaat ggccgacggt gaagacgcaa
     2401 acctgcaaag tcagctttac acggctaaac aactggtgag cgaattgatt ggcatggaca
     2461 gcaaactgtc cggcgtactt gatatgctgg aagaagctac catccagatt gctgaagcca
     2521 gcgatgaact gcgccactac tgcgatcgtc tggatctcga tcccaaccga ctgtttgaac
     2581 ttgaacagcg catctcaaaa cagatttcgc tggcacgtaa acatcacgtc agccctgagg
     2641 cattgccaca gtattaccag tcgctactgg aagaacagca gcaactggac gatcaggccg
     2701 actcacaaga aacgctcgcg ctggcggtaa cgaaacatca tcagcaggca ctggaaaccg
     2761 cgcacgcatt acacctgcaa cgccagcatt atgcaaatga gttggcacag ctgatcaccg
     2821 acagtatgca tgcgctctca atgccgcatg ggcagtttac gatcgatgtt aaatttgacg
     2881 agcatcacct gggcgctgac ggtgccgatc gtattgagtt tcgggtaacc accaacccag
     2941 gtcagccaat gcagcctatt gccaaagtcg catccggtgg tgaattgtca cgcatcgcat
     3001 tggcaattca ggtcatcacg gcgcgtaaaa tggaaacccc ggcactgatt tttgatgaag
     3061 tggatgtagg gattagcggt ccaacagcgg cagttgtcgg caaactgctg cgtcaacttg
     3121 gcgaatcaac tcaggtgatg tgtgttaccc acctgccaca agtcgcggga tgtggtcatc
     3181 aacactattt tgtcagcaaa gaaaccgatg gtgcgatgac agaaacacac atgcaatccc
     3241 tggataaaaa agcgcggtta caagagctgg cgcgcctgct tggtggcagt gaagtcacac
     3301 gtaacacact ggcgaatgcg aaagaactgc ttgcggcgta aacttttttc ctgcttcacg
     3361 gtcagagtaa acagcaaaac gccgtaggac cggaaagcaa aaggttttaa agtgatgaaa
     3421 ggtctattat catcggcata ttacagatga gccacgtact gctcgggccc gaaaaggaat
     3481 caaatcacta tgcgctgtaa aacgctgact gctgcagcag cagtactatt gatgttgacc
     3541 gcaggctgtt ccactctgga gcgagtggtt taccgtcctg acatcaacca ggggaattat
     3601 ctgaccgcta acgacgtatc caaaatacgc gttggcatga cgcaacaaca agttgcgtac
     3661 gcattgggta caccgttgat gtccgatcca tttggtacga atacctggtt ctatgtcttc
     3721 cgccagcaac caggtcatga aggtgtaacg cagcaaacgc tgacgctgac ctttaacagt
     3781 agcggtgtgt tgaccaacat tgataacaaa cctgcgctga gtggtaacta ataataaagt
     3841 tgctctcaaa gacgttaaaa aaggtgctca atgagcacct tttttctgtc tgttatttat
     3901 tcgctgattt ttctgctcgt tgcctgcgca gctctttcgg atcggcaatg agagggcgat
     3961 aaatctccac ccgatcgcca tcatgcacac tatcgctcag ttttgccgga cggctataaa
     4021 tgccgacttt gtttttagtt aaatcgatat cggtacgcaa ttccagcaag ccactggcgc
     4081 gaatagcttc ttcaaccgtc gctccctcct gcagcgtcac gcgctgcagg tactgcttct
     4141 caggtagcgc ataagccacc tcaacggcaa ttttacctgg cactgtaaac ctctttcgca
     4201 cgaaccgtaa aagcctggac catattagcc gccagctctt taaacacgcg accaaaggcg
     4261 agttcaatca acttattggt aaactcaaaa tcgagatgaa actcgatacg acacgcctcc
     4321 tggctcagcg gcgtaaactt ccatccaccg atcaatttct tgaacggccc atccaccaga
     4381 ttcataagaa tactttggtt actggtcaac tggttgcggg tagtaaacgt tttgctgatc
     4441 ccagccttag agacatctac cgccgcagtc atctgcccag gagtggactc cagaatccga
     4501 cttccggtac aacccggcaa aaactgagga taagactgaa cgtcattcac taactgatac
     4561 atttgctccg cgctgtaggg taccagtgcg gtccggctaa tctgaggcat aacaatttcc
     4621 atcaacaaaa atccaacaaa taatatcatt tatcctgctg taaaaaaaac gctatcccgg
     4681 cgctgggtaa catcgggttc atgctaagat agagccttgt cccccgcagg attgatatgg
     4741 ggtgttttcg atttcagatt accgatgatt cacgacgctt atgacgaaga aaaaagcaca
     4801 taaacctggt tcagcgacca tcgcgcttaa caagcgcgcc cggcacgaat actttatcga
     4861 agaagagttc gaggcgggac ttgccctgca aggctgggag gttaaatccc tgcgcgcagg
     4921 aaaagccaat atcagcgaca gctacgtcct tctgcgtgac ggagaggcat ttctgtttgg
     4981 cgctaacatc acgccaatgg ctgtggcctc cacgcatgtg gtgtgcgatc ctacccgtac
     5041 ccgcaagtta cttctcaacc agcgcgaact ggactcattg tacggtcgcg tcaatcgaga
     5101 aggctatacc gtagtggcgc tcgccctgta ctggaaaaat gcctggtgca aagtgaaaat
     5161 cggcgtcgcc aaaggtaaga aacagcacga taaacgttca gatatcaaag agcgcgaatg
     5221 gcaggtggat aaagcgcgta tcatgaaaaa cgcccaccgt taaacctgca ctccaattat
     5281 tgaccagttc ctcaccgcgc ctccctctcc ggcggcgcga atgaacatct tattggctat
     5341 cacatccgac acaaatgttg ccatcccatt gcttaatcga ataaaaatca ggctacatgg
     5401 gtgctaaatc tttaacgata acgccattga ggctggtcat ggcactcatg aatctggtat
     5461 acttaccgtt acacattggg gctgattctg gattcgacgg gatttgcgaa acccaaggtg
     5521 catgccgagg ggcggttggc ctcgtaaaaa gccgcaaaaa atagtcgcaa acgacgaaaa
     5581 ctacgcttta gcagcttaat aacctgctta gagccctctc tccctagcct ccgctcttag
     5641 gacggggatc aagagaggtc aaacccaaaa gagatcgcgt ggaagccctg cctggggttg
     5701 aagcgttaaa acttaatcag gctagtttgt tagtggcgtg tccgtccgca gctggcaagc
     5761 gaatgtaaag actgactaag catgtagtac cgaggacgta ggaatttcgg acgcgggttc
     5821 aactcccgcc agctccacca atcatgattg gacggtgcaa ggacaacacc aacaaaaaca
     5881 ggaagttaga agtctcagca agacaccgac cagacggtga ggagacataa aaggatacgc
     5941 aaaggagccg cggctcttgg tgatatgaaa gcccacagat gtgggctttt tcgttgatgg
     6001 tcagaacgac cagttcgcac cagccaccgc gttccacggg gattccacac cggcaccatg
     6061 gctatacccc accccgagat gtccgcttaa cgtactgctg aatgaggctt taatacctgc
     6121 ctggtatatt ccacgtctgc ccgacaaatc attgacgaaa ttaccgtcat tattcacctt
     6181 cacccggtta tcatcgacaa attctttgcg cacagccgcc ttcagccacg gctcaatttc
     6241 cat
//
LOCUS       DAPOZO010000174         6027 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPOZO010000174 DAPOZO010000000
VERSION     DAPOZO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.175"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..266)
                     /locus_tag="ROI21_004574"
                     /pseudo
     CDS             complement(1..266)
                     /locus_tag="ROI21_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(487..864)
                     /locus_tag="ROI21_004575"
     CDS             complement(487..864)
                     /locus_tag="ROI21_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113401.1"
                     /translation="MNQYIANYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQI
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSIDDELATVVATGLIEAIVTATDSNSLLWK
                     DVSKFLGDKSKSHALVWKDFGQE"
     gene            complement(864..5364)
                     /locus_tag="ROI21_004576"
                     /pseudo
     CDS             complement(864..5364)
                     /locus_tag="ROI21_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(5440..>6027)
                     /locus_tag="ROI21_004577"
     CDS             complement(5440..>6027)
                     /locus_tag="ROI21_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113402.1"
                     /translation="EKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIVKVG
                     SNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNN
                     VTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVN
                     GDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
BASE COUNT         1161 a         1965 c         1416 g         1485 t
ORIGIN      
        1 cactcgtgaa attttgcagg actgatacag gatacaactc tggcgatggt atcgtgaaca
       61 ggaataccat tttaaaaatc accgtattgt tttaaaaaat cgagatgtgt ttccccaaaa
      121 tcctctatat cttcccaacc ttccgcaccc gaaataacag cacaaatagt caacagtaga
      181 atatccgata atttatgttc cactttccag gcttgtctgt aatcggggat aatagaaata
      241 tgccccatca attttttaag ttctattttg ttctccttaa ttatgtaagg agtatttgat
      301 catgtataag caataaaaaa cagctttagg taatgaggaa tatctcaatt tttaagcata
      361 aaatgcgaat tgtttagtac aaaaaagcag cgttacgtga tctttccctg cacttcaccc
      421 taactttaat ttaacttcaa gaagtattaa ggcctcaata gcgaacttaa tacttcttaa
      481 agaatatcac tcctgaccaa aatctttcca cactaatgca tgagactttg atttatcacc
      541 caaaaattta cttacatctt tccaaagtaa agaattgcta tcggtcgcag tgactattgc
      601 ctctataagc cctgtagcta caactgtggc caattcatca tcaatcgaga ccataccatc
      661 ttcgacatgt ttaaatattt tttctctttc aatatcaccc atttcataaa aattttttac
      721 aatcgttttt ccaatctgag agaacaacat aaccaccggt ggagtttctg gatgccagta
      781 ttcattagct aatgagactg tctcagacaa aacagtggaa aacgatgcca ttttttctat
      841 ataattagca atatattggt tcattacttg cctcccaaag cattctgtaa atcctgaaga
      901 acgttttctg ctgctgctcg atcccctgga gatgctgttg gattcttatc caaccaatcc
      961 tgtaaagccc ttgtataatc ctgtgctttc aatacatgtc ctctaccacc aactttttca
     1021 cctgtggcca attcataacg aacagcatct gctgtgcttc cacttcctac ttttgcattt
     1081 ggccgataaa gatcgtctat aatatttttc aaatttgaat ctgtaacgtc aggttttccc
     1141 atcttctgtc gcaattcatc tttataccgt tcatgctgca tacgatctct tgcgctattc
     1201 tcatcttctg gtccccatcc tcccgggctt cccgtactgc caccacccag gcagttcttc
     1261 tcttcctccg tcaaatcctt cccgacattt ggattctctt ttgatgaatc atcatttcca
     1321 aacacgctgg aaaaagcacc atagggatct cgccagaatt ccatttgact ttgtgcattt
     1381 aataattcgg cttcaccctg catctccata cttttctcaa tcgcgtcccc aacagcctcg
     1441 cgattgttgt atattattat accaccacca ataaccaccg taccgactgc cgcaccgact
     1501 aacatccccg gagggcctcc taccgaaccg atctctgtac ctacttcaga accagttccc
     1561 atggcagtta atgctcctgc gcccaacgcc gcacttaatc ccagcggatc cagccgttgc
     1621 accgggttca gtggatacgc atacaggttc catcccccct tcagcccaat cggatcctgg
     1681 gtgatatacc tcccctgtag cgggtcatag taccggtgac ggttgtagta cagccccgac
     1741 tcctcatcat actgctgccc cggcagacgg agtgactgtt gaaggtgctg cgtgctttcc
     1801 tctcccagca ggtttcccca ctcgtcatac tccccgcgcc acgccgtttc cccttccggg
     1861 ctgataagcg cctgcggcag cccacggtgg tcacagtggt aaagatgaag tttcctctcc
     1921 gggatgtatt ccgcctccag ttgcgccgcc atctgttccg ccgtcaaccc acactgtgca
     1981 agccactgct ggctttcttc actcacgctg ccctgccgca gttcccgctc cagcctcccc
     2041 agcatcaccg ccagctccgc cggtagcgtc acacccgtgt cctcctgcaa cacctccgcc
     2101 aggctacggt gccgcgcctt cgcctgttcg ccattctctg tttcgattct caggagcggc
     2161 gtgaagctcc ccggctggta taccgtctgg atacgcgtgg tgtcggtctg taccgtcgtc
     2221 agcctgtcgc cgtcccaccc gtaccaggtc acctccggtt tacgcgacag cgacatccac
     2281 cccgtcaggt cacgctcccg ccgccacacc cgttttcccg tccggcggcc cagcgggtca
     2341 tagaggtagc ggctctccac ctgtggctcg ccgtgctgta tccgcgtgta gaacaccagg
     2401 cggtgctggc tgtcgtaatg gtagtggtgc gtccgctcgt cgtgcatccg tatcacccct
     2461 tccgggatac ggtccgtctt ctccgccagc ctgccgtatt catcgtagcg gtagacatag
     2521 tgcgcatctt ccgtgatacg gttatccggc catgccgtga gggtgctgtc cgggtgcagt
     2581 tccgggtccg gcagccggtt tcctgccggg tccgtcgcat acgggatatc aatgtccagg
     2641 ttcgctgccg tggtgtggac gcccgtcagc cttcccgtgt cgctgtaacg gtactcccgg
     2701 ctctcctgcg ggccgctgat gcgaattagc tgtccgttgt cgttccagtc gtagtcacgg
     2761 tcaagctgcg gaaggttcag gtgcctgctc cggagctggc cgctggtatt ccaggcggtg
     2821 gcaagctcat atgcctcccc gccgaagctg cgggccgtct cacggtgcag ccggtcccgc
     2881 gtgtactcga ccagtggcgt accacccagc ttcattcccg caagataacc gctgccatac
     2941 gtcagccact ccaccggcgg cagaccgtcc ggctcctgac gggtcgccag cccctgttcg
     3001 ctgtacgcgt gcttcgtctc atgctcccac agcatctccc ccgtctccgg gttctccacc
     3061 gtctgccgtt cgcccgtcag gcggccttta tcgtcatagc cgtagtggac cgacacgcgg
     3121 tggtcttcac tggtatggct gatggtggtt aaccacccgt actcatcata ctgccactgc
     3181 tccgccgggt cgccgttcac cgtccggtgc gtgatgcggt ccgacgcatc gtagtgccag
     3241 agggtgataa gcccctcgtc ttcactgtgg acaagctgcc ctgtggcgct gtaccggtaa
     3301 cgctgcgtcc tgccgtcaaa gccgcgctgc tccgtcagcc tgtccaccgg gtcataccgg
     3361 aacgtggact ggctgccgtt ctcgttggtc agcacggtga tgcgcccggc agcgtcatac
     3421 cccatgctgc gcgtcagacc gccttccgtc acactcaccg gacgcccgcg tttatcatat
     3481 tcgatggtgc tgcgtttacc gtccggtgat atcgttgctg tcaggtcgcc tgcggcgctg
     3541 tactcataac gggtctcccg cccctgcgca tccttctgac tgaccagttg gccacgcggg
     3601 ttataactgc tgtaagtgct gatgccttcc tcccggtgaa cggcgatttg ttgaccgcac
     3661 cggtcatact cataccgcgt cgtgtacccc gagcagtccg taaacgcctg aagctgaccg
     3721 taccggtttc gcgttattct ccgcgtcacc ccggttccgt cttccaccgc gccgggcagg
     3781 ctactgcggg ggggagtcat aaaaccagcg cgtaatgtta ccgttgcgcg agatctcttc
     3841 cgccagcctg ccctgacggt catatttccg gctgctgcgc agcccgtccg ggtacgtcac
     3901 tgacgtcacc tgccgctggc tgttgtagcc ataccgcacc gtcctgccgt ccggcagtat
     3961 cactgaagtc agctttccag acgccatatg cagccggtac tccgtccgcc gtcccgccgc
     4021 atccgtctgc gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc
     4081 cgcatgttcc ttcttcacca cccgcttcag gccaccttcg ccttccgtgt acagcacctc
     4141 ccgcctgttc aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc
     4201 cagcccctcc gggttgacct gctccgtcac ccggccggta tcatcatacc ggtagcggct
     4261 ctccggcctc cccgcataat ggtgcgccac catccgcccg gcgtgcgcca catcataagt
     4321 aaacccgcgc acctgcgtcc cgctgcggtc atacaccgcc cgcagttctc cgctggccgt
     4381 gtacgtgtag cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt
     4441 cagccacacc gcctccagcc ggataccgtt atcggcaccg tattctgtac cggcgggcag
     4501 cgtatcgggg aaaacctgag aagaggaaac agaacgggga ccggcagggg aagataagga
     4561 agactcgcgc tgtttacgga aggcttccgc ccgctgcgcc tgcgtgctca gcgccaggtg
     4621 aaaacgacgg cccgcaccgt ccgtcacccc cgtcaccgcg cccgccacgt caccctccgc
     4681 cgcccggtgg aaggccagcg tccgcccgaa gccgtccacc acgcccgtca gcacccggta
     4741 tgcgggcggc tccggcggca gcacctcgtc cacttccggc acccgctccg gccagccgag
     4801 tatccaccac ggcccctgaa ggctgtttgt cgccaggtac acatgcggac tcaggcgaac
     4861 atcttccggc agcacctgcc agagcgcgct tagcggctgc gaactgtgct gcgccgccac
     4921 cccgccccgc gccagccaga gtgactcgct gcggctgtag cttatctcgc cgggaaacag
     4981 cggctcaaag tgaatgctcc tgccgccgtt gtcgttgagt atcagttccc gctcatgaac
     5041 ctgtaagcgg atatcgaagg gggctttcca gccgggacca aacaccccca ccggcgcgga
     5101 cgtcctggtc cggtagctgc tgtaggcgcg ggaaagaata aacggcaacg ggccgggcag
     5161 cgcaaggtcc gtctcgcccg gcagcacctt cgctcccagc agcgggttta ccggattgcc
     5221 atatttcggc gtggcttccc ctgtgggaca caccgagcag gccacaccgg ttggcgcacc
     5281 tatcagcacc cctgctgaac cctgcacaat atccagtccc ttgcgggtca tgtcaccctg
     5341 ccgcgccgcc ggttttccac ccatgaatca attccttctg cttttaatat ggtttatctg
     5401 gtcactgcac cagttccgtt ttaatttttt gcacctgact caaaattgcc gcatatccgg
     5461 cattccgggg ggtaaatctt tcggcacagg cgcatccggt acaggttgtg tggcaccaca
     5521 gttccagtta atcaccggcg catcaccgtt cacatccgac tctccttcca gatgaatgtg
     5581 cgtaccattg agaaatatgc ttccgtcctt cgtcagcacc aaccttgcct taccgcagca
     5641 gagttccaga tgctcaccgg cagaataaac tgctgtttgt ccggtgtttt ccttcatcgt
     5701 cttgcccacc gagaaggtga cgttattccc tacattgacg ctgtagccca ttcccaccat
     5761 cagtgattta tgcagcccta cctgtgagga ctgcaacaac gccaccgagg tattcatgat
     5821 gccgcctacc gtcgtctggt aagccacgcc caccgtcagc gcacgaatga tccccacctt
     5881 ttcaacctgg tttgaaccca ctttgacaat ctggttgctg cctaccgttt ccacctggtt
     5941 gacaccaacc gtctgtatct gattaacgcc aatgttctgg atctggttat tggtaacggt
     6001 gatcgcctgg ttgttaccta ttttttc
//
LOCUS       DAPOZO010000175         5956 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPOZO010000175 DAPOZO010000000
VERSION     DAPOZO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.176"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..970
                     /locus_tag="ROI21_004578"
     CDS             <1..970
                     /locus_tag="ROI21_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDX5113403.1"
                     /translation="KLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFES
                     KVGLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPT
                     GIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAQPKDESL
                     VASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKE
                     VRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLD
                     LVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQTAESED"
     gene            complement(967..2103)
                     /locus_tag="ROI21_004579"
     CDS             complement(967..2103)
                     /locus_tag="ROI21_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDX5113404.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKVAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(2109..2432)
                     /locus_tag="ROI21_004580"
     CDS             complement(2109..2432)
                     /locus_tag="ROI21_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDX5113405.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2614..2757
                     /locus_tag="ROI21_004581"
     CDS             2614..2757
                     /locus_tag="ROI21_004581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113406.1"
                     /translation="MNKNRKTSTMQIKRIKPSIIYRLLLIGLGTPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2976..4514)
                     /gene="aldB"
                     /locus_tag="ROI21_004582"
     CDS             complement(2976..4514)
                     /gene="aldB"
                     /locus_tag="ROI21_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDX5113407.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMELVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4715..5866)
                     /gene="yiaY"
                     /locus_tag="ROI21_004583"
     CDS             complement(4715..5866)
                     /gene="yiaY"
                     /locus_tag="ROI21_004583"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDX5113408.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMVGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     NIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1530 a         1547 c         1404 g         1475 t
ORIGIN      
        1 aaaattacaa cttaagtacc gggagttaaa aattatctcg gtaatcgctg ccagtgaaaa
       61 tatcagccat gccgcgactg tacttggcat cgcacaggcc aacgtcagca aatatcttgc
      121 tgattttgaa tcaaaagtgg gtttaaaagt ctttgaccga accacccgac agctgatgct
      181 cacacctttt ggcaccgtgc tgttaccgta catcaacgac atgctggaca gaaacgagca
      241 gcttaataat tttattgcag attataagca tgagaaacgt ggtcgggtca ctatctatgc
      301 accaaccggc ataattacct atttatccaa acatgtaatc gataaaatta aagatatcgg
      361 tgatatcacc ttatcgctaa aaacctgcaa tcttgagcgt aatgcctttt atgaaggcgt
      421 cgaatttccc gatgattgtg atgttttaat cagctacgcc caaccaaaag atgaatcgct
      481 ggttgccagt tttattaccc aatatgccgt aaccgcttat gccagccagc gctatcttga
      541 gaaacatccc attagccgtc ctgacgaact cgaacatcac tcctgtattt tgatcgactc
      601 gatgatgatc gacgacgcga atatctggcg cttcaacgtc gccgggagta aagaggtgcg
      661 ggattatcgc gtgaagggaa attacgtttg cgacaacacg caatctgcgc tggagctggc
      721 acgaaatcac ctggggattg tgtttgcgcc agacaaaagt gtgcagagcg acctgcaaga
      781 cggcacgctg gtgccctgct ttcagcaccc gtatgaatgg tggctggatc tggtggctat
      841 cttccgtaaa cgggaatacc agccctggcg ggtccagtat gtgctggatg aaatgctgcg
      901 tgaaattcgc caccagcttg ctcagtcgca gcaactgcgg cctgaacaaa cggcggaaag
      961 cgaagattaa tgatcgagat aaaggtaatg cacccagctg gtcatgcgta acagtacttt
     1021 gcgcatcacc gagacatggc tgaaatgatc agagtagacc gccacctggg cgtaaatacc
     1081 gtctggtaat gcgctcaaat cagtatgctc atccagttca atggtcgcga taacgccatc
     1141 tgaacccggc gctgtgttta acgtctgtaa ggtgccggtc gactgataag ctccgccggg
     1201 aacggctgga ctaatggctg ccaccttacc gctgaatacc ttacctggca gagcattaaa
     1261 caccacttcc gcatcgtcgc caggagccag tcgcagcaag gagttctgac ggaactgcgc
     1321 cacgatttgt cgtttctgat cgggtataaa caccatcacc ggacgtagcg gcagcgacgc
     1381 ggcataggta cccggacgaa tcagtacctg ggtcacataa ccatcgctcg gcgcacgcac
     1441 tatcgtctgc tcaaggttat attttgcttc cgcgagctgt gctttcaggc tggcgatttg
     1501 agaatgttca cccaacacca ggctatccag ctggctctgg atctgttttt gttccgccgc
     1561 cgatgacttc acggaggctt cctgcgccag ataattttgc cgcgccacat cgatatcgcg
     1621 ttctgaaaac gggtttactt tcgcctggct gccacgtgcg taacgctgat actctttagc
     1681 gaacttatcc cgcgtggcct ttgcctgctg agtattcgcc gccatctcat ctaactctgc
     1741 gcccaacgcc cgctgtttat gttctgcggt aacgatatcc gccatcagcc gatccacccg
     1801 agcctgataa cgcgtcgggt ccagtcgaaa tagcacctca ccttttttaa tcagcgtatt
     1861 tttcttgtcc gtcacttcga tcaccacgcc tgtcacctgt gggacaacag gaatagaaat
     1921 aaccgctttt tgcgctttaa aggtatacgg atggttatag ttcattaaca gaattagacc
     1981 gctgacaata aatatgcctc ccagggccgc tgtgggaatg gtccatttat ttacaggaat
     2041 tttaaagatc ttaaacattg cccatgcgaa agccacatag gtcaaaataa tcagtaaatc
     2101 cataagtatt actcggcaga agaggatttg atgtcggcga gctgtttctc cagcccggca
     2161 atacgctgtt gtagttgcac aacggaggag tcatggtttt gcattcccca tccccgctcc
     2221 gggcggtaaa gcgtggccca aatccacaaa aatggccaga taacatgtaa cgtaaaaaga
     2281 cttacccaac cagcaacatg aatggcgtcg gcatgtggat gattacgttt tttagcaatc
     2341 aggtagggaa tatcatgtaa aataataatc ccatagaaga tcaccagaaa tacaaaaata
     2401 agcactccca gtgcaaaata atccaggaac atatttccct cagagaatat aaaaaagaac
     2461 aattaacgca tattattaaa tgccatgatg cagtgcaatt tccttattta tataacgaca
     2521 tatggttttt tatatagttt ctgaagtgtg tgcctggctt ttcaacaggt cgaagcttat
     2581 catattgaaa taatgtaact attgaccaaa tttatgaata aaaaccgtaa aacatccaca
     2641 atgcagatta agcgtattaa accttcaatt atctaccgtt tattgctgat tggcctcgga
     2701 acgccaatgg tgatttatgg cctggttcgc ccgctcacca tcgaaacgcg agattaaact
     2761 cctggcaaaa cacgccccag aaacgcactt ctgttggcgt agtctgggtt attgcgcacg
     2821 taggtttaaa acgtaaattg taacaacgtg cgctttgttt atgccggatg cggcgtgtac
     2881 gccttatccg gcctacggtc aggtccccgt aggcatgata aaacgcgtaa gcgtcgcatc
     2941 aggcaatgaa tacccaatgc gaccagcttc ttatatcaga acagccccaa cggtttatcc
     3001 gaatagctca ccagcaggca cttggtttgc tggtaatgct caagcatcat cttatgggtt
     3061 tcgcgtccga tgcccgactg tttgtagccg ccaaacgccg catgtgccgg gtaagcgtga
     3121 taacagttgg tccacacgcg cccagcctga atgccgcgcc ccatcttata ggccagatta
     3181 ccgttgcggc tccagacacc cgcgcccagg ccatattgcg tgtcgttcgc cagctccagc
     3241 gcttcttcca tcgttttgaa ggtggtcacc gccagcaccg gaccaaaaat ttcttcctgg
     3301 aacacgcgca tattattctg accaaacaga atcgtcggtt cgaggtagta gccgtctttc
     3361 agttcgcctt ccagcagctt gcgccgcccg ccggtgagca cgtctgcgcc ctctttttta
     3421 ccgatatcaa tgtagttgag gatggtttcc agttgcccgt gagaaacctg cgcccccatt
     3481 tgcgtcacgc tgtcgagcgg gttaccgctg cgaatgcttt cgacacggcg gatggcgcgt
     3541 tccataaagc gttcgtagat agactcctgc actaaagcac ggcttggaca ggtgcaaact
     3601 tcgccctggt taaaggcaaa cagtgcaaag ccttccagcg ctttatcgaa aaaggcatct
     3661 tcttcatcca tcacatcagc aaagaagata tttggggatt tgccgcccag ctccagcgtc
     3721 accggaataa tgttttgcgt ggcgtactgc ataatttgtt ggcccacttc cgttgagccg
     3781 gtaaacgcca ctttggcgat gcgtttcgag gtcgccagat attcgccaat ttccccacca
     3841 gcgccgttaa ccacgttcac cacgcccggc ggcagtaaat caccgacgag ttccattagc
     3901 agcagtacag aaagcggggt aagacgtgcg ggtttcagca ccacacagtt gcccgccgcc
     3961 agcgcgggag ccattttcca gctcgccatc agcagcggga agttccacgg aataatctgc
     4021 cccaccacgc ctaacggttc gtggaagtga taagccacag tttcgctatc cacttcacta
     4081 ataccgcctt cctgcgcccg aatacacgag gcaaaatagc gaaaatggtc aatcgccagc
     4141 ggcacatcag cagcactggt ttcgcgaatg ggtttgccgt tatcccaggt ttcagcggtc
     4201 gctaacagtt cgaggttctg ctccatccga tcggcaatct taaacagaat cgccgcgcga
     4261 tcctgcaccg aagtatgcgc ccatttatct ttcactttgt gcgcagcatc cagcgccaga
     4321 tcgatgtctc gtttgcccga agacgccact tcgcacagca gctgcccggt caccggcgtc
     4381 aggttctggt aatactcacc atcggcaggg gctacccatt cgccgccaat aaagttgtcg
     4441 tagcgggctt ttaacttgag ggggaaacca tactcgccgg gcttaatctg tgctgaaggg
     4501 ggattattgg tcatgatcgt ctccttgcgg tgtgaggtat aacaagggta gacgtgactg
     4561 gcgaaatctt cgccagtcgg taacagcttt acgacggcta tcacgaattt acgggcaagg
     4621 attacagaga taactaaaac tctctcaaaa atcgcaaatt ttgagatagt taaaaaaatg
     4681 gctgacaatt agccagccat tgggggaatg atgattacat cgcagcgcga taaattgcca
     4741 caatctcttc gtgagttgcc tgaatcgggt tagtaaagcc acacgcatct ttcagggcat
     4801 tggtggccag taccgcgaaa tcctcttctt tcacgttcag gtcacgcagg cctgccggga
     4861 tattcacttt cttcgccagt tcacggatgg cattgatgca cgcttctgcg ccttccgcgt
     4921 cgtttttacc agtcacgttc acgcccattg cagcggcaca gtcacgcaga cgtgctgcag
     4981 cgactttgct attgaatacc tgaacgtgtg gcagcagaac agcattacat acaccgtgtg
     5041 gcaggttgta gaaaccgccc agctggtgcg ccatcgcatg aacataaccc agagaagcat
     5101 tattgaacgc cataccggcg aggaactggg cataagccat tgcttcacgc gctttagcat
     5161 tgctgccatc ttcaacggct aacggcaggt tttcggcaat catggtcacg gctttcagtg
     5221 cgcaagcgtc agtgatcggc gtggcggcaa tagaaacata cgcttcgata gcgtgcgtta
     5281 aggcatccat acccgttgcg gcggtcagtg acttcggcat accgaccatc agagatgagt
     5341 cattgacaga aagcaacgga gttacatgtt tatcaacaat cgccattttg atatgacgcg
     5401 cttcgtcagt gatgatgcag aaacgggtca tttcagaggc cgtacccgcc gtggtattga
     5461 tggcgatcat cggcagctgc ggttttgcag agcggtcaac gccttcgtaa tcgcgaatat
     5521 cgccgccatt ggctgccact agcgcaatac cttttgcgca gtcgtgcgga gaaccaccgc
     5581 ctaaggagat cacgctatcg caattatttt ctttaagtaa tttcaaacct gcggcgacgt
     5641 tttccgtggt tgggttaggt tgggtgccat cataaataac gctaaaaata ttgcgttctt
     5701 ccagtgcttt ttgcacatca cccgccatac ctaatttcgt taacatattg tcagtgacaa
     5761 ttaaggtacg ggtaaatcca taatctgcca tcatattcat tgcatcattc aatgaatcag
     5821 caccaataac attcacggaa ggaataaaaa aggttgaagc tgccataaca cactcctgaa
     5881 agtgtaaaaa agggcgggca gcttactgag gattttcatt cgaaaatatg atcagtatta
     5941 ttgtttatca aaatta
//
LOCUS       DAPOZO010000176         5840 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPOZO010000176 DAPOZO010000000
VERSION     DAPOZO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.177"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..373
                     /locus_tag="ROI21_004584"
     CDS             <1..373
                     /locus_tag="ROI21_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDX5113409.1"
                     /translation="VPRLAGAKSGGVFQAVLGAAVMAVAIWMPGVGIMASNLLFSLGA
                     SMTLGGVAQMLAPKPRTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRV
                     VSQEISTADEGDGGQVVVIGR"
     gene            434..3913
                     /locus_tag="ROI21_004585"
     CDS             434..3913
                     /locus_tag="ROI21_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDX5113410.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMLVWNGQTLTFVQDRPSDKVWTYNHSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPVAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRITDIRQVETTASYLGTGLYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGRLTAKNADISGSVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVG
                     KAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGARHERENSDTYSSCRLI
                     VKKNGAEIYNRTALDNTLIYTGVIDMPAGSGVMTLEFSVSAWWVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            3981..4580
                     /locus_tag="ROI21_004586"
     CDS             3981..4580
                     /locus_tag="ROI21_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDX5113411.1"
                     /translation="MRKLCAVILSTVVWLVAAGTPASAAEHQSTLSAGYLQTHTDMPG
                     NDDLKGINVKYRYEFTDTLGLVTSFSYANAKDEQKTHYSDTRWHEDSVRNRWFSMMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDSKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            4645..>5840
                     /locus_tag="ROI21_004587"
     CDS             4645..>5840
                     /locus_tag="ROI21_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDX5113412.1"
                     /translation="MAAVKISGVLKDCAGKPIQNCTIQLKAKRNSTTVLVNTVASENP
                     DEAGRYSMDVEHGQYSVILLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEEDVMPE
                     ALRRFEAMVEEVARNAEAASQSAAAAKKSETAAASSKNAAKTSETNAANSAQAAATSK
                     TASANSATAAKKSETNAKNSETAAKTSETNAKSSQTAAKTSETNAKASETAAKNSQAA
                     AAESESAAAGSATSAAGSATAAANSQKAAKTSETNAKSSQTAAKTSETNAKASETAAK
                     SSQDAAAQSESAAASSASAAAASATASANSQKAAKTSETNAKVSETAAANSAKASAAS
                     QTAAKASEDAAREYASQAAEPYKYVLQPLPDVWIPFNDSLDMITGFSPSYKKIVIGDD
                     EITMPG"
BASE COUNT         1459 a         1439 c         1772 g         1170 t
ORIGIN      
        1 cgtgccgcgt ctggcaggag ccaaaagtgg cggtgtgttt caggctgtgc tgggggcggc
       61 tgttatggcg gttgctatat ggatgccggg ggtaggaatt atggcgagta atctgctgtt
      121 ttctctcggt gccagtatga cgcttggcgg tgttgcacag atgctggccc ctaaacccag
      181 aactccccgc acacagacaa cggataacgg caaacagaac acctattttt cttcactgga
      241 taacatggtt gcccagggca atgttctgcc ggttctgtac ggtgaaatgc gcgtggggtc
      301 acgcgtggtt tctcaggaga tcagcacggc agacgaaggg gatggtggtc aggttgtggt
      361 gattggtcgc tgatgcaaaa tgttttatgt gaaaccgcct ccgggcggtt ttatcgttta
      421 tggagcatga cgaatgggta aaggcagcag taaggggcat accccgcgcg aagcgaagga
      481 caacctgaag tccacgcagc tgctgagtgt gatcgatgcc atcagcgaag ggccggttga
      541 aggtccggtg gatggattaa aaagcgtgct gctgaacagt acgccggtgc tggacagtga
      601 ggggaatacc aatatatccg gcgtcacggt ggtgttccgg gccggtgagc aggagcagac
      661 accgccggag ggatttgaat cctccggctc cgagacggtg ctcggtacag aagtgaaata
      721 tgacacgccg ataacccgga ccatcacgtc ggcaaacatt gaccgtctgc gttttacttt
      781 cggcgtgcag gcactggtgg aaaccacctc aaagggggac aggaatccat cggaagtccg
      841 cctgctggtt cagatacaac gtaacggtgg ctgggtgacg gaaaaagaca tcaccattaa
      901 gggtaaaacc acttcacagt atctggcctc ggttgtggtg gataacctgc cgccgcgccc
      961 gtttaatatc cggatgcgca ggatgacgcc ggacagcacc acagaccagc tgcagaacaa
     1021 aacgctctgg tcgtcataca ctgaaatcat cgatgtgaaa cagtgctacc cgaacacggc
     1081 actggtcggc gtgcaggtgg actcggaaca gttcggcagc cagcaggtga gccgtaatta
     1141 tcatcttcgc gggcgcattc tgcaggtgcc atcgaactat aacccgcaga ctcggcaata
     1201 cagcggtatc tgggacggaa cgtttaagcc agcatacagc aataacatgg cctggtgtct
     1261 gtgggatatg ctgacccatc cacgctacgg catggggaaa cgtcttggtg cggcggatgt
     1321 ggacaaatgg gcgctgtatg tcatcggcca gtactgcgac cagtcagtgc cggacggctt
     1381 tggcggcacg gagccgcgca tcacctgtaa tgcgtacctg accacacagc gcaaggcgtg
     1441 ggatgtgctc agtgatttct gttcggcgat gcgctgtatg ctggtatgga acgggcagac
     1501 gctgacgttc gtgcaggacc gaccgtcgga taaggtgtgg acctataacc acagtaatgt
     1561 ggtgatgccg gatgatggcg cgccgttccg ctacagcttc agcgccctga aggaccgcca
     1621 taatgccgtt gaggtgaact ggattgaccc ggataacggc tgggagacgg cgacagagct
     1681 tgttgaagat acgcaggcca ttctccgtta cggtcgtaac gtcacgaaga tggatgcctt
     1741 tggctgtacc agccgggggc aggcgcaccg cgccgggctg tggctgatta aaacggaact
     1801 gctggaaacg cagaccgtgg acttcagcgt gggtgccgaa gggcttcgcc atgtaccggg
     1861 cgatgtcatt gaaatctgtg atgatgacta tgccggtatc agcaccggtg gtcgcgtgct
     1921 ggcggtgaac agccagaccc ggacgctgac gctcgaccgt gaaatcacgc tgccatcctc
     1981 cggtaccacg ctgataagcc tggttgacgg aagtggtaat ccggtcagcg tggaggttca
     2041 gtccgtcacc gacggactta aggtgaaagt gaaccgggtt cctgacggcg ttgcagaata
     2101 cagtgtgtgg gggctgaagc tgccgacgct gcgccagcgc ctgttccgct gtgtgagtat
     2161 ccgtgagaac gatgacggca cgtatgccat cactgccgtg cagcatgtac cggaaaaaga
     2221 agccatcgtg gataacgggg cgcactttga cggcgaccag agcggcacgg tgaatggtgt
     2281 cacgccgcca gcggtgcagc acctgaccgc cgaagtcacc gcagacagcg gggaatatca
     2341 ggtgctggcg cgctgggaca cgccgaaggt ggtgaagggc gtgagcttcc tgcttcgcct
     2401 gaccgtggca gcggatgacg gcagtgagcg gctggtcagc acggcccgga cgacggaaac
     2461 cacataccgc ttcacgcaac tggcgctggg gcggtacacg ctgacagtcc gggcggtaaa
     2521 tgcgtgggga cagcagggcg atccggcgtc ggtatcgttc cggattgccg caccggtagc
     2581 accgtcgcgg attgagctga cgccggggta ttttcagata accgccacgc cgcatcttgc
     2641 cgtttatgac ccgacggtac agtttgagtt ctggttctcg gaaaagcgga ttaccgatat
     2701 caggcaggtt gaaaccacag ccagctatct tggcacgggg ctgtactgga tagccgccag
     2761 tatcaatatc aaaccgggcc atgattatta tttttacgtt cgcagtgtga acaccgttgg
     2821 caaatcggca ttcgtggagg ctgttggtca gccgagtgat gatgcatccg gctatctgga
     2881 ttttttcaaa ggcgagatag ggaaaaccca tctggctcag gagctgtgga cgcagattga
     2941 taacggtcag cttgcgcctg acctggctga aatcaggacg tccattacgg atgtcagcaa
     3001 tgaaatcacg cagaccgtca ataagaaact ggaagaccag agtgcagcga tccagcagat
     3061 acagaaggtt caggttgata caaataataa cctgaacagc atgtgggcag tgaagctgca
     3121 gcagatgcag gacggacgcc tttatattgc gggtatcggt gccggtattg agaacacccc
     3181 cgacggcatg cagagtcagg tgctgctggc ggcggacagg attgcgatga ttaatcctgc
     3241 gaatggcaac acaaagccga tgtttgttgg tcagggcgat cagatattca tgaatgaagt
     3301 gttcctgaaa tatctgacgg ctcccaccat taccagcggc ggtaatcctc cggcattttc
     3361 cctgacaccg gacgggcgac tgacggcgaa aaatgccgat atcagcggta gcgtgaatgc
     3421 gaactccggg acgctcaaca acgtcacgat taacgagaac tgtcgggttc tgggaaaact
     3481 gtccgcgaac cagattgaag gcgatctcgt taaaacagtg ggcaaagctt tcccccggga
     3541 ctcccgtgca ccggagcggt ggccatcagg gaccattacc gtcagggttt atgacgatca
     3601 gccgtttgac cggcaaattg ttattccagc ggtggctttc agcggtgcca gacatgaacg
     3661 ggagaatagc gatacttatt cgtcatgccg cctgatagtg aagaaaaacg gggctgaaat
     3721 ttataaccga acggctctgg ataatactct gatttacacg ggtgttattg atatgcctgc
     3781 aggcagtggc gtaatgacac tggagttttc tgtatcagca tggtgggtaa atggctggta
     3841 tcccacagca agtatcagcg atttgctggt tgttgtgatg aagaaagcca ctgcaggcat
     3901 cacgattagc tgaattttat aacccagata cgggcgccag aaatggcgcc ttttttattg
     3961 cagaaaagcg agaggtaatt atgcgtaaat tatgtgctgt tattctgtcc acagtagtct
     4021 ggctggtcgc cgctggtacg ccagcgagcg cagcagagca tcagtccaca ctaagcgccg
     4081 ggtatcttca gacccatact gatatgcccg gcaacgatga cctgaagggc attaacgtga
     4141 aataccgtta tgaatttacg gacacgctgg ggctggtgac gtcattcagt tatgccaatg
     4201 ccaaagatga gcaaaaaacg cattacagcg atacccgctg gcatgaagat tcagtgcgta
     4261 accgctggtt cagcatgatg gcggggccgt ctgtacgcgt gaatgaatgg ttcagtgctt
     4321 atgcgatggc aggtgtggct tacagccgtg tttcaacttt ttccggggat tatctccgcg
     4381 taactgacag caaggggaaa acgcacgatg tgctgaccgg aagtgatgac ggtcgccaca
     4441 gcaacacgtc tctggcgtgg ggggctggcg tgcagtttaa cccgaccgaa tccgtggcca
     4501 ttgatattgc ttatgaaggc tccggcagtg gtgactggcg cactgacggt ttcatcgtgg
     4561 gtgtcggtta taaattctga ttagccaggt aacacagtgt tatgacagcc cgccggttca
     4621 ggcgggcttt tttgtggagt ggatatggca gcagtaaaaa tctcaggtgt gctgaaagat
     4681 tgtgcgggaa aaccaataca gaactgcact attcaactga aggcaaagcg taacagcacc
     4741 acggtactgg tgaacacggt ggcctctgaa aatccggatg aagccgggcg ttacagcatg
     4801 gatgttgagc atggccagta cagcgtcatc ctgctggttg aaggttttcc gccttcacat
     4861 gccgggacca ttaccgtcta tgaaggctcc agaccaggta cgctgaatga ttttctcggt
     4921 gccatgacgg aagaggatgt catgccggag gcattgcgtc gttttgaggc aatggtggaa
     4981 gaagtggcac gcaacgccga agccgcctct cagagcgcag cggcggcaaa gaaatccgaa
     5041 actgcagcgg catcatcgaa gaacgcggcg aaaacctcag aaacgaatgc agctaatagt
     5101 gcacaggcgg cagcgacctc aaagactgca tcggcaaact ctgcgacagc agccaaaaaa
     5161 tcagaaacca acgcgaaaaa cagtgagaca gccgcaaaga cgagcgaaac caacgcaaag
     5221 tccagccaga cggcagcgaa aaccagcgaa acgaatgcca aagccagtga aactgcggca
     5281 aaaaacagcc aggctgcagc agctgaaagc gagagcgcgg cagccggttc tgcgacttca
     5341 gcagctggat cagcaactgc tgcggctaac agccagaaag ctgcgaagac gagtgaaact
     5401 aacgcaaagt ccagccagac ggcagcgaag accagcgaaa cgaatgccaa agccagtgaa
     5461 actgcggcga aaagcagtca ggatgcagcg gcccaaagcg agagtgccgc agccagttct
     5521 gcaagcgcgg cggctgcttc tgccactgca tcagctaaca gtcaaaaagc agcaaaaacc
     5581 agtgaaacca acgcaaaggt gagcgaaaca gcggctgcga actcagcgaa agcatcggca
     5641 gcaagccaga cggcagctaa agcaagcgaa gatgcagcca gagagtacgc aagccaggct
     5701 gcggagccgt ataaatatgt cttacagccg ctgcctgatg tgtggatacc atttaacgat
     5761 tcgctggata tgattacggg cttttcgcca tcatataaaa aaattgttat tggtgatgat
     5821 gaaataacga tgcctggcga
//
LOCUS       DAPOZO010000177         5811 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPOZO010000177 DAPOZO010000000
VERSION     DAPOZO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.178"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..345
                     /locus_tag="ROI21_004588"
     CDS             <1..345
                     /locus_tag="ROI21_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDX5113413.1"
                     /translation="MLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSALADRGLVQSMS
                     RKGNCLDNAVMENFFGHLKEEMYYRRDYRNVEELENAVNEYITYWNQKRIKLSLGGLS
                     PVEYRTEHQKAG"
     gene            complement(445..1305)
                     /locus_tag="ROI21_004589"
     CDS             complement(445..1305)
                     /locus_tag="ROI21_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDX5113414.1"
                     /translation="MNIAIVTINQENAATASWQAAQDFSGCTLAHWKIEPQPAVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPSGMQQLNIVPGALPDWLVSIENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHAECQ"
     gene            complement(1302..2081)
                     /locus_tag="ROI21_004590"
     CDS             complement(1302..2081)
                     /locus_tag="ROI21_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDX5113415.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTSMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQYQNPLELIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTENGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAVGKAAIIRKTVAAESPA
                     MQCLQLARAEQQRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(2059..3468)
                     /locus_tag="ROI21_004591"
     CDS             complement(2059..3468)
                     /locus_tag="ROI21_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5113416.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGIKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(3490..4944)
                     /locus_tag="ROI21_004592"
     CDS             complement(3490..4944)
                     /locus_tag="ROI21_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDX5113417.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWTLLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(5014..5799)
                     /locus_tag="ROI21_004593"
     CDS             complement(5014..5799)
                     /locus_tag="ROI21_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDX5113418.1"
                     /translation="MSIESLNALSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQSVEVDFMQVDITAEGAPQKIIAACCERFGTVDILINNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
BASE COUNT         1458 a         1597 c         1410 g         1346 t
ORIGIN      
        1 atgttaaata aggggctgga aagccttgct gaaggggaga aaccgctgct ccacagtgat
       61 caaggatggc attaccggat aaaaagttat cagtccgccc tggcggacag agggttagtg
      121 cagagcatgt cgcgcaaggg caactgcctt gataatgcgg taatggagaa cttctttggt
      181 cacctgaaag aagaaatgta ctatcgccgg gactacagaa atgtagaaga gctggaaaat
      241 gccgttaacg aatacataac ttactggaat caaaaaagaa taaaactcag tcttggggga
      301 ctcagtccgg tagaataccg gactgagcat caaaaagccg gttaactgaa actgtccagg
      361 ttttggggtt cagttcattg acgcgtctta tcaggcttac aagtccccag cccgaaaccg
      421 taggcaggat catccggctg cacgttattg gcattctgcg tgaatgtttg tcaccagtgc
      481 ctccagcacc actttccagt catcaaccac gccaacatct gcctgtgaaa ataccgctgc
      541 gctggcgtca tgattaatcg ccaccacaaa cttgctattg cgtacgcccg ccattaacgc
      601 tgctgcgccg gaagcgccaa caacaataca cacctcaggt gccagcagat gtccagaaat
      661 accaatcact ttttcggcat caacgccacc gttcatcact cgtgcccgac tgtagcccac
      721 ttcggctccc agcttttctg ccagcatagc gatttcctgg ttatctgcct ctccgccctg
      781 ccccaccacc agcacccgtc tggcctcagc taatgggtcg cgggtaacat ttttcaggtt
      841 ttctatgcta accagccagt cgggaagcgc acctgggaca atgttcagtt gctgcatacc
      901 agagggcaac gtggcgtttt tatctgctcc cgcctgacgt gccagcgaaa ggcatagcgg
      961 gcgtttttct gtttgcagcg tcgccgttag cgcatttccc cagtgggatt tgcgcacgct
     1021 tacggttgga atatctaacg aagtcacctg gcagatgctt gcaccatgta aacgccaggc
     1081 cagccgggtc gacagttcat cgccaaacgt tcccggcgga aagagaacaa cgtccgctgg
     1141 cgttcgctgc cactgttcca ccagcgcatc cagcacctgt tccgccacag cgggctgagg
     1201 ttcaatcttc cagtgcgcca gggtacaacc gctgaaatcc tgcgccgcct gccagctcgc
     1261 tgttgcggca ttctcctgat ttattgtcac aattgcgata ttcatggctg catcctttgg
     1321 cgtaaatagt cctgccatag cttttgcgct ttttctgcta ccgtttgccc atcaatcagc
     1381 gtcgctccgc gttgttgctc tgctcttgcc agttgcagac actgcatcgc tggcgattcg
     1441 gcagcaacag ttttacggat aattgctgct ttccccaccg ccattcgctg acgcattccc
     1501 ggtactggca aggccacttc accgcactga cgcacggcaa tcaccgcagg caagcgaacc
     1561 cggcaacagc gcagcccgtt ttcagtacgc tgttcaaggg tgataaacgg cgcgtcgaga
     1621 gtaaaacgct ccacctgggt gaagcagggc cagcccagca tttccgccag caaaaatggc
     1681 gtttgcccat tctgcccttc gctgctttgg cagccagtaa ttatcagctc cagcggattc
     1741 tggtactgcc attcggcaat atggcgagcg acaaattccg gcgcaaagcg cagatctgcc
     1801 gccgtctcca gcaatacagc ttcctcaaac ccaagagcca taagatagcg cagccagtgc
     1861 aacgcccgtt catcccccat gctcaacgcg gttaaagaca tcgacgtacc gttttttctc
     1921 tgcgccaaca gcagcgcggc ggcagcctgt tcatcagcac cgagcaaact tcgcagaagc
     1981 gaaacatccg gtccgctatt accctgagcc gccgcctgcc actctttttc cgccagcatt
     2041 ccggcatccg gttcggcttt aaacgctaac agaatgttca ttcgcacctc caacatttcc
     2101 cgccgccacc agcgggagtg ctttggtttc tggtgcccat agccaggtca cgacaaaacc
     2161 aaccaacaac actgccgcca ggagcaataa ggtgacttgc attccccact gcgccagcac
     2221 ccacggcagc aggccagtac ttaccgccgc gcccaaccga ctcatggcgg tggcaaaacc
     2281 gacgcccagc gagcgaatgt cagtaggaaa actttccgca ggcaaaatgc ccaccagatt
     2341 actgactgcc gaaatggtgg tgctgaagag aacaaaaagc agcagcgtta atgaactgcc
     2401 ggaaggcaaa caagccatca ctaccagcgt tgccgccagc agcaaaaaac ttcccagcaa
     2461 aaatttgcga tgtgccagca ggtgcgtcag aactaatccc agcagcgcac ccacaattaa
     2521 caacgcatta agcatcaggc tagcggtcaa cgcatcttcc agaccaatag tctgggcgat
     2581 agttggcagc caggtataaa tcacaaacca tgggattacg aggcagacaa agaagacgct
     2641 gttaaacgct gtgcgccgcc agtagcgcga agagaacaag gttttgatgt gtttatgggt
     2701 cgccgttgcc acttcatcgc ccagtaaaac atggggacca aaatagcgat gcacgatagc
     2761 gtgagcttct gcaaaacgcc cctggcgcag tagccagcgt ggcgattctg gcgttcccca
     2821 gcgtaataac gtaatcaaca atgcgggcag agctgccgat gccagcagcc agcgccaggc
     2881 ctccgggctt tcgctaataa agtgatgtcc ggcaatactt gccagcacat agcctacagt
     2941 ccacaccacg ctgaacgcgc ccagcaaaat accgcggtgg cggcgcgggg aaaattcagc
     3001 cagcaaggtg tgaccgactg agtaatcgcc tcccagacca atgccaatca aaatgcgcag
     3061 tccaataaga tgctctggcg tggtggcaaa aaattgtaaa aacgaagcaa gcgtaatcag
     3121 caaaaagctg aaggtgaaga ttttttgtcg cccaatatgg tcggagatcc agcccagaac
     3181 caggctgcca agaaacaaac caaggagtgc tgagccgcca atcattcccg ccataaacgg
     3241 cgtcagttgc atggcgggcg taagctgaat aatggcgtaa ccaatgacgc cgaggacata
     3301 accgtcggtc aggtgtgcgc cgaaagtgag cgcagcaatg cggcagtgaa aacggttaag
     3361 tggtaaatca tccattcgca ccggtgaagt gttcatcttt atcccttcat tgtccggtaa
     3421 taagaacggc ttctcgcctg agaagccgaa aaggcaacgc cccgtcatcg cggaacgctg
     3481 cctgacacat tatttttcaa tcggatagat agtacccgta ttcataatgc cgttaggatc
     3541 gaactgcttt ttcagacctt ccagcaacgt ccacgcgctg ccgtgttcca gcttgctcca
     3601 gtgaacgcga tgtttaccaa tgccgtggtg gtgcaccatc gaaccaccga ggcgaatggt
     3661 ttcttcgcag atgattttgt tgagcggatt gtggtacttg tcgatttcct cttccggctt
     3721 acagtcaaca acgttgtagt cgtaaacgaa gtacatgttg gtgccgttct ggtagctgtg
     3781 agaggagtga ccgcccagca tggtgatgtc gtcggcgtgc gggaactcgg tacgaatacg
     3841 gttaataacg ctttcgtaga tttcgtgaat gcagctccag cagccggaca cttcggtggt
     3901 aaagcccatg ttgccggttt tgaggatctg cacacgttcg gcagctactt tatccggtcc
     3961 ccagttcagg ttgttaaacc aggtttcgat cagcttgctg tccactcgct ggcattgcgg
     4021 gtaacgtgcc acgatttcag caatcccttc gcccgtcgcc ttcgcaatgc gagggttacc
     4081 ttcagccata aagatcagca cgcattttcc gtcagcaaaa tgggtgaagt gttgggtgcc
     4141 atcttcagcg tcatacaaac gagcgatcga cggacgatac ccttccacca tgatttcacg
     4201 caggatgttg aagccggttt tcatgtcttc caggatatag ccgtagaaga ggttgttttc
     4261 cggggtgaac ttaaagattt tcacagttac ttcagtgata tagcacaatg caccttcgtt
     4321 gccgatgatg atgtgacgaa tgtccgggcc tgccgcgcgg cgtggtacgt ttttaatgcg
     4381 tgttacggtg ccatcagcca atactgcttc cagaccaacg accatatctt cgattgcgcc
     4441 gtagagtgtg gagaactgcc cgatactgcg ggttgctacc aggccgccca tctgcgccag
     4501 cggctttgac tgcggagaat gccccgtggt gtaacctttt tcacgcaacg cgttttccag
     4561 cacttccagc ggaactccgc attgcgccgt ggcctgcata ttctcaatat caatattaat
     4621 gatttgattc atggcagagc cgtcgagcac caccgagttt tccacaacgg tttccagccc
     4681 accttcggta gcggaagcac cggtacgcgg cacaccgtta attttgtgcg cattcataaa
     4741 attcagcaca cgggatactt gttctgtgga accgagtttt acgaccgctg ccggaatcgg
     4801 caaagtataa atgccatgaa tatccggaaa tttgcgaaaa cggtcaatac tgttcttctt
     4861 taatactgtt tcatcggtaa ttacgcgatc tgcaccaaca atttccttta gctggtcgac
     4921 aatcgctgcg cgagataaag acatagtaat tccttcctga taataaaaat ggaataatca
     4981 ttaataagag aaaacagaaa ccaccgttat ggattagcgc actaaataac caccatcaac
     5041 cactaataaa tgtccgttga cataattcga tgccggactt gcgaggaata cggctgcgcc
     5101 cattaaatcc tgagtatcgc cccaacggtt tgccggaata tgatcaagaa cgcgctgatt
     5161 ggtttctgga ttactgcgtg tcgccagcgt aatatctgtt gcataatagc caggggcaat
     5221 accatttacc tgaatattat attgacctag ttcatcacag taagctttgg tgaatccggc
     5281 aagagcatgt ttagtggcag aatatgcagg tgaccattgt ccacctaagt aagagaataa
     5341 tgaacagata ttaatgattt taccgctttt ctgcgggatc ataatttttg cagcttcata
     5401 gcttaactcg aatgcggcgg tcaggttcac atcaatcatc gggtcccagt cggcacgacc
     5461 gaagtccagc accttattca gcttacaaat accggcattg ttaatcagaa tatcgacggt
     5521 accgaaacgt tcgcaacagg cggcgattat cttctgcggc gctccttctg cggtgatatc
     5581 cacctgcatg aagtccacct caacactctg tttttcaatc atttcctttg tttcgccgtt
     5641 atctttaacg aagcttggaa taaagatatt tgcgcctgct ttggccagcg ccatggcaaa
     5701 tgcctggcct aaaccgctat tcccgccggt aacaattgcg gttttacctt tcagggagaa
     5761 aaaatccatt gagagcgcat tgagagattc gattgacata gttagctcca a
//
LOCUS       DAPOZO010000178         5736 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPOZO010000178 DAPOZO010000000
VERSION     DAPOZO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.179"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            215..544
                     /locus_tag="ROI21_004594"
     CDS             215..544
                     /locus_tag="ROI21_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDX5113419.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            531..923
                     /gene="tnpB"
                     /locus_tag="ROI21_004595"
     CDS             531..923
                     /gene="tnpB"
                     /locus_tag="ROI21_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDX5113420.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKARSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTQNTLLTQ"
     gene            930..2531
                     /locus_tag="ROI21_004596"
     CDS             930..2531
                     /locus_tag="ROI21_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HDX5113421.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCGTLAGMQRSLFEEDVDADIAALTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKIIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEELWSWLEEQEPQCSPGRALHKAIAYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDV
                     LMRLPEWPEERLAELLPLEGFTFSG"
     gene            2894..3571
                     /locus_tag="ROI21_004597"
     CDS             2894..3571
                     /locus_tag="ROI21_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDX5113422.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            3571..3918
                     /gene="tnpB"
                     /locus_tag="ROI21_004598"
     CDS             3571..3918
                     /gene="tnpB"
                     /locus_tag="ROI21_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDX5113423.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            3938..5504
                     /locus_tag="ROI21_004599"
                     /pseudo
     CDS             3938..5504
                     /locus_tag="ROI21_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         1384 a         1570 c         1563 g         1219 t
ORIGIN      
        1 tataacggtc aggccacact gaatgtgacc ttctgacaga accatcgcct ctctgtggtc
       61 ccggtcatca tgaccgggac ccggaccggc gcaacggatc ttcaacgcca cattcgctgg
      121 cattaacgtg tcgttacggg gaagtcatct tcacagggat cttcggtcag ttagtattac
      181 cgacatccat tcaccaactt accggagata atcgatgtca aaaccccgat ggaccattga
      241 acagaaaagg cagcacgttg ctgcctggcg cgccagtggt cttacacgtc agcaatactg
      301 tgaactcaac gatattccct tcacatcact tcgcgaatgg ccgcaggatg tcgcaaaagc
      361 tgaacgccgg gcaaatgaac cgaccgttct tcccgttcat attgcgccac cactgcatgc
      421 tgatgtacca cagcccgtca cgaatgagcc agtcacgctc ttccttcccg gtggagtacg
      481 gatgtgctgt cagccgtcac aacttaccga tgtcttcagg gcactcaaat atgctcagtc
      541 ctgataatgt cttcatcgca attaaacctg ttgacatgcg ccggggcatc gactcactga
      601 cgcagtatat acaggatgaa ctccggtcga catggcatga gggagccgcc ttcgtctttg
      661 ttaacaaagc ccgttcgcgt atcaaagttc tccggtggga taaacacggg gtgtggttgt
      721 gtacccgccg tctgcacaaa ggcagcttcc gctggccacg tgcaaatgac gctgcctggc
      781 acctcactcc cgacgagttt aactggctga ttgccggtgt tgactggcag caggttaagg
      841 gacatgacct gacgaaatgg gtctggcaga atgaacctga actgcgccct gaaaacacgc
      901 aaaatacact gctaactcag tgaaaaataa tgaatatccg tatctggagt ggtatactcc
      961 cctgtatgga tatctccgct ctcaacacca cgaatgacat cgaaaaactg cgtgctatgg
     1021 cacttgccat ggtacaagaa gtcatgtcgg agaatgccga aaaagagcgg gaattactgg
     1081 agaaaagccg gcgcatccag cttctggaag aaatgctgaa actggttcgt caacagcgct
     1141 tcggaaaaaa atgtggaacg ctggctggta tgcaacgctc cctgttcgaa gaggatgttg
     1201 atgccgatat cgccgcgctt accgcacatc tggataaact gctcccgcaa tcccctgaag
     1261 aagacgaaaa agcgtcccgt tcacgcccga tacgcaaacc cttaccggtt catcttccac
     1321 gggtggaaaa aattatccag ccggacactg accattgccc tgaatgtgac gagccgctgc
     1381 actatatccg cgatgcggtg agtgaaaagc tggagtatat tcccgctcac tttgtggtga
     1441 accgttatgt ccgtccacaa tacagttgtc cctgttgcca gaaggtgttc agcggtgaaa
     1501 tgccggcaca tatcctcccg aaaagtgccg ttgagccatc agtcatcgca caggtgatca
     1561 tcaataaata cggtgaccac ctgcctctgt atcgccagca acaggtcttt gcccgttcag
     1621 atgtcgggct gcccgtcagt tcgatggctg acatggttgg cgcggcgggt gccgcattat
     1681 ctcccctggc ggcgttactc catcgcgagt tgataaaccg tccggtggtg catgcagatg
     1741 agactaccct gaagatcctg aacacgaaga aaggcggtaa atcctgctcc ggttatctgt
     1801 gggcatacgt cagtggagaa aggacgggac cgtcagttgt gtgcttcgac tgccggaccg
     1861 gacgtagcca tgagtatcct gaaaactggc ttcagggctg gggcgggacg ctggttgtcg
     1921 acggacataa agcttaccgg actctggcaa acaaagtgcc ggagatcacg ctggccggat
     1981 gctgggccca tgcccgcagg ggcttcgccg acctgtataa aatcagtaaa gatccacggg
     2041 ctgccatagc cgtgaagaaa atcgcggggt tgtaccgtct tgagaagaag atcagtagcc
     2101 gccccgtgga aaaaatccgc cagtggcgac agcgttatgc ccgtccgata ctggaagaac
     2161 tgtggtcatg gcttgaagag caggaaccgc aatgttctcc gggaagggca ttacacaaag
     2221 ccattgccta tgcgctgtct catcgcgtgg aactgagccg cttcctggaa gatggtgcgg
     2281 tgccgctgga taataatgtg tgtgaacggg ccatcaaaaa cgtggttctg ggcagaaaat
     2341 cgtggctgtt cgccggttcg cagatggcgg gagaacgcgc cgcgcaaata atgagcttgc
     2401 tggaaaccgc gaaacgcaac ggtctggagc cgcatgcctg gttgacagac gtcctgatgc
     2461 gtctgccgga gtggccggag gagcgactgg cagagttgct gcctcttgag ggatttacct
     2521 tctccgggtg agtgatacct gccgtcaggt gttcgtgcac cgggccataa cctgcagtcg
     2581 ggagttgaac tcctgacggc aggaaatgag ccagaccagc aaccacgcgg gctgacagcg
     2641 aataagaacc tgaatcagcc cggagtcatt cccgggtcag tacatcatca cctgctatta
     2701 atccccgagt cagtacttcg cggcctgacg cgatcaccct actcagtact tcagagcccg
     2761 cctccggtac ctgcctggcg atcgcccctg tgcaacaaaa taaatcaacc gttatcaaac
     2821 cacgatcgtt cgtatgcgcc gtaaccggct catttagacc gtctggtctg tttcctccgg
     2881 ctctacaaaa ataatgtcca tcatttttaa tggacactat cgtatgaaac accggacctg
     2941 gatcactgaa gctttacgtc ttcactttga agaacattta ccccgggttg tggccgggcg
     3001 tcgcctgggt gtaccaaaat caacagtttg tagtatgttc gtgcgctttc ggagagctgg
     3061 cctttcgtgg cctttgcccg caggcatgtc ggagcaggaa cttgatgcct gcctttacgg
     3121 acaattttcc acggtaccag tcgtacgtcc tgaaagcacc gttatatccg aagcccccgt
     3181 ggtaaaaaaa cgtccccggc ggcccaactt cccttatgag tttaaaatcg ccttagtgga
     3241 gcagtcactg cagcccggag cctgtgtggc gcagatcgcc cgggaaaacg gaatcaacga
     3301 taacctgctc ttcaactggc gccatcaata ccggaaaggt ggcctgctgc cttccggaaa
     3361 aaatatgccg gcactgcttc ccgtgacgtt aacgccggag ccggataata aaatcccggc
     3421 ccccgcacag gaaccagagc agataaatac accgtccgac agtctgtgtt gtgagctggt
     3481 tctgccggcc ggaactctca ggcttaaagg taaactgacg ccggcgttat tacagacact
     3541 tatccgcgaa ataaaaggga gcagccactg atgatatctc tccctgcagg ttcgcgtatc
     3601 tggctggttg caggtatcac cgatatgcga aatggcttta acggcctggc atcaaaagtt
     3661 cagaacgtcc tgaaggatga cccgttctcc ggacacctgt tcatcttccg cggacgccgg
     3721 ggtgaccaga taaaagtgtt gtgggctgac agtgacggac tgtgcctctt caccaaacgc
     3781 ctggagcggg gccgcttcgt ctggccagtc acccgtgacg gcaaggtgca ccttactccg
     3841 gctcagttat ccatgcttct tgaaggtatc aactggaagc acccgaaacg aacggaacgc
     3901 gctggaatcc gcatataacc cgttgtaaag tgaggatatg gacacctcac ttgctcatga
     3961 gaacgcccgc ctgcgggcac tgttgcagac gcaacaggac accatccgcc agatggctga
     4021 atacaaccgc ctgctctcac agcgggtggc ggcttatgct tccgaaatca accggctgaa
     4081 ggcgctggtt gcgaaactgc aacgtatgca gttcggtaaa agctcagaaa aacttcgtgc
     4141 aaaaaccgaa cggcagatac aggaagcaca ggagcgaatc agcgcacttc aggaagaaat
     4201 ggcggaaacg ctgggtgagc aatatgaccc ggtactgcca tccgccctgc gccagtcttc
     4261 agcccgtaaa ccgttaccgg cctcacttcc ccgtgaaacc cgggttatcc ggccggaaga
     4321 ggaatgctgt cctgcctgtg gtggtgaact cagttctctg ggatgtgatg tgtcagagca
     4381 actggagctt atcagcagcg cctttaaggt tatcgaaaca caacgtccga aacaggcctg
     4441 ttgccggtgc gaccatatcg tgcaggcacc agtaccttca aaacccattg cacgcagtta
     4501 tgccggagcg gggcttctgg cccatgttgt caccgggaaa tatgcagacc atctgccgtt
     4561 ataccgccag tcagaaatat accgtcgtca gggagtggag ctgagccgtg ccacactggg
     4621 gcgctggaca ggtgctgttg ctgaactgct ggagccgctg tatgacgtcc tgcgccagta
     4681 tgtgctgatg cccggtaaag tccatgctga tgatatcccc gtcccggtcc aggagccggg
     4741 cagcggtaaa acccggacag cccggctgtg ggtctacgtc cgtgatgacc gtaacgccgg
     4801 ttcacagatg cccccggcgg tctggttcgc gtacagtccg gaccggaaag gtatccatcc
     4861 acaaaatcac ctggccggtt acagcggtgt gcttcaggcc gatgcttacg gtggttacct
     4921 tatacgaatc cggcagaata acggaagccg cgtgtatggc tcatgcccgg agaaaaatcc
     4981 acgatgtgca tgcaagagcg cccacctaca tcaccacgga agccctgcag cgtatcggtg
     5041 aactgtatgc catcgaggca gaggtccggg gctgttcagc agaacagcgt ctggcggcaa
     5101 gaaaagccag agccgcgcca ctgatgcagt cactgtatga ctggatacag caacagatga
     5161 aaacactgtc gcgtcactca gatacggcaa aagcgttcgc atacctgctg aaacagtggg
     5221 atgcactgaa cgtgtactgc agtaatggct gggtggaaat cgacaacaac atcgcagaga
     5281 acgccttacg gggagtggcc gtaggccgga aaaactggat gttcgcgggt tccgacagcg
     5341 gtggtgaaca tgcggcggtg ttgtactcgc tgatcggcac atgccgtctg aacaatgtgg
     5401 agccagaaaa gtggctgcgt tacgtcattg aacatatcca ggactggccg gcaaaccggg
     5461 tacgcgatct gttgccctgg aaagttgatc tgagctctca gtaaatatca atacggttct
     5521 gacgagtcgc ttactatgcg ccgccgatga cgcgcgcctg aaaaacgcgt caatatgagt
     5581 tccccggaag cttacgcatt aacaataaca tgatattcat cacggagtga ctatgttaca
     5641 gatagtcggc gcgctgatcc tgctgatcgc aggatttgcc attcttcgcc ttttgttcag
     5701 agcattaatc agcacggcgt ctgcgctggc agggct
//
LOCUS       DAPOZO010000179         5712 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPOZO010000179 DAPOZO010000000
VERSION     DAPOZO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.180"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..963
                     /gene="ompC"
                     /locus_tag="ROI21_004600"
     CDS             <1..963
                     /gene="ompC"
                     /locus_tag="ROI21_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDX5113424.1"
                     /translation="SDNDSKDGDKTYMRLGFKGETQVTDQLTGYGQWEYQIQGNEPES
                     DNSSWTRVAFAGLKFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYDSDNFMQQRG
                     NGFATYRNTDFFGLVDGLDFAVQYQGKNGSAHGEGMTTNGRDDVFEQNGDGVGGSITY
                     NYEGFGIGAAVSSSKRTWDQNNTGLIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNA
                     TRVGSLGWANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGA
                     TYYFNKNMSTYVDYKINLLDDNRFTRDAGINTDDIVALGLVYQF"
     gene            1075..2130
                     /gene="apbE"
                     /locus_tag="ROI21_004601"
     CDS             1075..2130
                     /gene="apbE"
                     /locus_tag="ROI21_004601"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDX5113425.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKTQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LSVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEDDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2204..3268
                     /gene="ada"
                     /locus_tag="ROI21_004602"
     CDS             2204..3268
                     /gene="ada"
                     /locus_tag="ROI21_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDX5113426.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3268..3918
                     /gene="alkB"
                     /locus_tag="ROI21_004603"
     CDS             3268..3918
                     /gene="alkB"
                     /locus_tag="ROI21_004603"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDX5113427.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            3994..5637
                     /gene="yojI"
                     /locus_tag="ROI21_004604"
     CDS             3994..5637
                     /gene="yojI"
                     /locus_tag="ROI21_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDX5113428.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYTPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLIEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELIGEERDAASRDAVARTA"
BASE COUNT         1326 a         1448 c         1559 g         1379 t
ORIGIN      
        1 tccgataacg actctaaaga tggcgacaag acctacatgc gtcttggctt caaaggtgaa
       61 actcaggtta ctgatcaact gaccggttat ggtcagtggg aatatcagat tcaaggtaac
      121 gaacctgaaa gtgacaatag ttcctggacc cgtgtggcat tcgctggtct gaaattccag
      181 gacgttggtt cttttgacta tggtcgtaac tacggcgttg tttacgatgt tacttcctgg
      241 accgacgtac tgccagaatt cggtggtgat acctacgatt ctgataactt catgcagcag
      301 cgtggtaacg gtttcgcgac ctaccgtaac accgacttct tcggtctggt tgacggtctt
      361 gattttgctg ttcagtatca gggtaaaaac ggtagtgctc atggcgaagg tatgaccact
      421 aatggccgtg atgatgtatt tgagcagaac ggtgacggtg ttggtggttc tattacttac
      481 aattatgaag gctttggtat cggtgctgca gtttccagct ccaaacgtac ctgggatcag
      541 aacaacactg gcctgatcgg tactggcgac cgtgctgaaa cctacactgg tggtctgaaa
      601 tacgacgcta acaacatcta cctggctgct cagtacaccc agacctacaa cgcaactcgc
      661 gtaggttccc tgggttgggc gaacaaagca cagaacttcg aagctgttgc tcagtaccag
      721 ttcgacttcg gtctgcgtcc gtccctggct tacctgcagt ctaaaggtaa aaacctgggt
      781 cgtggctacg acgacgaaga tatcctgaaa tatgttgatg ttggtgcgac ctactacttc
      841 aacaaaaaca tgtccaccta cgttgactac aaaatcaacc tgctggacga caaccgcttc
      901 acccgtgacg ctggcatcaa cactgatgac atcgtagctc tgggtctggt ttaccagttc
      961 taatctcgat tgatatcgaa caaagggcct gcgggccctt ttttcattgt tttcagcgta
     1021 caaacacagt tttttggtgt actcttgcga ccgttcgcat gaggataatc acgtatggaa
     1081 ataagcttta cccgcgtggc actgctggct gccgcgctct tctttgttgg ttgcgatcaa
     1141 aaaacacaac ccgccaaaac ccacgctact gaagttaccg ttcttgaagg caaaaccatg
     1201 ggtaccttct ggcgtgccag cattccgggc attgacgcca aacgcagtgc cgaacttaaa
     1261 gaaaagattc agacccagct ggacgccgac gatcagctgc tttcgaccta taaaaaagat
     1321 tccgcgctga tgcgatttaa cgactcgcaa agtttatcgc cgtggccggt aagtgaagcg
     1381 atggccgata tcgtcaccac ctcgctgcgc attggcgcaa ggaccgatgg tgcgatggat
     1441 ataaccgtcg ggccgctggt gaatctgtgg ggctttggcc cggaacaaca gccggttcaa
     1501 attccgagcc aggaacagat cgatgcgatg aaagccaaaa ccggcttaca gcacctgtcg
     1561 gtcattaatc agtcgcatca gcaatatctg caaaaagacc tgccggattt atatgtcgat
     1621 ctctccactg tcggtgaagg ttatgcggcg gatcacctgg cacgcttgat ggagcaggaa
     1681 gggatttccc gctatctggt atcggtgggc ggcgcgctga acagccgtgg tatgaacggt
     1741 gaaggccagc cgtggcgggt agcgattcaa aaaccaactg ataaagaaaa tgcggttcag
     1801 gccgtggtgg atatcaacgg tcatggcatt agcacctctg gcagctaccg taactattac
     1861 gaactggacg gcaaacgtct ttcccatgtt atcgatccgc aaaccgggcg tcccatcgaa
     1921 cacaatctgg tatccgtgac ggtgattgct ccgacggcgc tggaagccga tgcctgggat
     1981 actggcttga tggtactcgg gccggagaaa gccaaagagg ttgttcgccg ggaagggctg
     2041 gcggtttata tgatcaccaa agaagacgat agctttaaaa cctggatgtc accacagttt
     2101 aaaagcttcc ttgtcagcga aaaaaattaa agcgcaagat tgttggtttt tgcgtgatgg
     2161 tgaccgggca gactaaaggc tatccttaac cagggagctg attatgaaaa acgccacatg
     2221 cttaactgac gatcaacgct ggcaatctgt cttagcccgc gacccgaatg ccgacggcga
     2281 attcgttttc gccgtgcgca ccacgggtat cttttgccgt ccgtcttgcc gcgccagaca
     2341 tgccttgcgg gaaaatgtct ccttctacgc aaatgccagc gaggcactcg ccgccggatt
     2401 tcgcccctgc aaacgttgtc agccagacaa agccaatgcc cagcaacatc ggttggataa
     2461 aattacccac gcctgccgac ttctggaaca ggaaacgcct gtaacgctgg aagccttagc
     2521 cgatcaggtg gcgatgagtc cgttccatct gcatcgattg tttaaagcca ccaccgggat
     2581 gacaccgaaa gcctggcaac aggcctggcg cgctcgccgt ttgcgcgagt cgttagcgaa
     2641 aggggagagc gtgactacat ctattcttaa cgccggattc cccgacagca gcagttacta
     2701 ccgcaaagcc gacgaaacgc tgggcatgac ggctaaacaa ttccgtcacg gtggcgaaaa
     2761 tctggcggtg cgttacgcgc tggctgattg tgagctgggc cgttgcctgg tggcagaaag
     2821 cgagcggggg atttgcgcga tattgctggg cgatgatgac gcgacactaa tcagcgagtt
     2881 gcagcagatg tttcccgctg ccgacaacgc gcctgccgat ttgatgtttc agcaacatgt
     2941 gcgtgaagtg atcgccagcc tcaatcaacg cgatacgccg ctgacgttac cgctggatat
     3001 tcgcggcact gcttttcagc aacaagtctg gcaggcactg cgcacgatac cttgtggtga
     3061 aaccgtcagt tatcagcaac tggcgaacgc catcggcaaa ccgaaagcgg tacgtgccgt
     3121 tgccagcgcc tgtgccgcca acaagctggc tatcataata ccctgtcatc gggtggtccg
     3181 tggtgatggc acactttccg gttaccgctg gggcgtgtcg cgtaaagcgc aactgctgcg
     3241 ccgcgaagct gagaatgagg agaggtaatg ttggatctgt ttgccgatgc tgaaccgtgg
     3301 caagagccac tggcggctgg tgcggtaatt ttacggcgtt ttgcttttaa cgctgcggag
     3361 caactgatcc gcgatattaa tgatgttgcc agccagtcgc cgtttcgcca gatggtcact
     3421 ccaggcggat ataccatgtc ggtggcgatg accaactgtg ggcatctggg ctggacgacc
     3481 catcggcaag gttatctcta ttcgcccatt gatccgcaaa caaataaacc gtggcccgcc
     3541 atgccgcaga gttttcataa tttatgtcaa cgtgcggcta cggcggcggg ctatccagat
     3601 ttccagccag atgcttgtct tatcaaccgc tacgctcctg gcgcgaaact gtcgctgcat
     3661 caggataaag acgaaccgga tctgcgcgcg ccgattgttt ctgtttctct gggcttaccc
     3721 gcgatttttc aatttggcgg cctgaaacga aatgatccgc taaaacgttt gttgttggaa
     3781 catggcgatg tggtggtatg gggcggtgaa tcgcggctgt tttatcatgg cattcaaccg
     3841 ttgaaagcgg ggtttcatcc actcaccacc gactgccgct acaacctgac attccgtcag
     3901 gcaggtaaaa aagaataaaa ataagaatta ttattgctgt gtgcgcgaag atgtttaaac
     3961 tgcgggctgc aattcattgt ccgggttttc tgcatggaac ttcttgtact tgtctggcgg
     4021 cagtatcgct ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc ggcattaggc
     4081 attggcttaa ttgcttttat caatcagcgc cttatcgaaa cggcggatac cagtctgctg
     4141 gtgttgccgg agtttctggg attattgctg ctgttgatgg cagtcactct cggatcgcaa
     4201 ctggcgctca ccactttggg gcatcacttc gtttaccgac tgcgtagtga atttatcaag
     4261 cggattctgg atacccatgt tgagcgcatt gaacaacttg gtagcgcctc gttgttggcg
     4321 gggttaacca gcgatgtgcg caatatcacc attgcttttg tgcgtctgcc ggaactggtg
     4381 caggggatca ttctcactat cggttcagcg gcgtatctgt ggatgctgtc gggcaaaatg
     4441 ttgctggtaa cggctatctg gatggcgatc accatctggg gcggttttgt gctggtggcg
     4501 cgggtgtaca aacatatggc gaccctgcgt gaaaccgaag ataagctgta cacggatttt
     4561 caaaccgtac tcgaagggcg caaagagctg actctgaacc gtgaacgcgc cgagtatgtg
     4621 tttaacaacc tctacactcc tgatgcgcaa gagtatcgcc atcatattat ccgcgcagac
     4681 accttccatc ttagtgccgt gaactggtca aacatcatga tgctgggcgc aatcggcctg
     4741 gtgttctgga tggcgaacag tctcggttgg gctgatacca acgttgccgc gacctattcg
     4801 ttgacgctgt tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt accgacgctg
     4861 ctgacggcgc aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc tttcaaagca
     4921 gagtttccgc gcccgcaggc gtttcccaac tggcaaacgc tggagctgcg taacgtgacg
     4981 tttgcttatc aggataacgc gttttccgtt ggtccgatta atctcaccat caaacgtggc
     5041 gagctgctgt ttctgattgg cggcaacggt agcggaaaat cgacgctggc gatgttgctg
     5101 acgggcttgt atcagccaca aagcggcgaa atcttgctgg atggcaaacc tgtcagcggc
     5161 gaacaaccgg aagattatcg caaactgttt tcggcagtgt ttaccgatgt ctggctgttc
     5221 gatcaactgc tggggccgga gggtcaaccc gctaacccgc aactgattga gaagtggctg
     5281 gcgcaactga aaatggctca taagcttgag ttaagcaacg ggcgtattgt taacctgaag
     5341 ttatcgaaag ggcagaaaaa acgcgtcgcg ctgttgctgg cgctggcaga agaacgcgat
     5401 attatcctgc tggatgaatg ggcggcggat caggacccac acttccgtcg tgagttttat
     5461 caggtgttgc tgccgctgat gcaggagatg ggtaaaacta ttttcgccat cagtcatgat
     5521 gatcattact ttatccacgc cgaccgcctg ctggaaatgc gcaacgggca acttagcgag
     5581 ctgataggcg aagagcgcga tgctgcttcg cgtgatgccg ttgcccggac ggcataatat
     5641 tacgacgctg acctggcgga ctgctgtcgc caggtcaata tgctaaaaat cccttcattt
     5701 ctaataaccc ta
//
LOCUS       DAPOZO010000180         5686 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPOZO010000180 DAPOZO010000000
VERSION     DAPOZO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.181"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            500..1402
                     /locus_tag="ROI21_004605"
     CDS             500..1402
                     /locus_tag="ROI21_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDX5113429.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTDAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISRQGENFIQVDF
                     DTPWCQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            1406..1710
                     /locus_tag="ROI21_004606"
                     /pseudo
     CDS             1406..1710
                     /locus_tag="ROI21_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1787..2470
                     /locus_tag="ROI21_004607"
     CDS             1787..2470
                     /locus_tag="ROI21_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDX5113430.1"
                     /translation="MSRFVLGNCIDVMTRIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            2471..2692
                     /locus_tag="ROI21_004608"
     CDS             2471..2692
                     /locus_tag="ROI21_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113431.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            2706..3140
                     /locus_tag="ROI21_004609"
     CDS             2706..3140
                     /locus_tag="ROI21_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDX5113432.1"
                     /translation="MYGTCETLCRELAAKYPGDAPLMLVIWSPEEIQALADGMEISLT
                     DHEIRTVLARMEDIPEDQRTESGISSAAVMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARTLLKNNTHENN"
     gene            3191..3967
                     /locus_tag="ROI21_004610"
     CDS             3191..3967
                     /locus_tag="ROI21_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113433.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIY
                     VRLDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSECIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            4507..4809
                     /locus_tag="ROI21_004611"
     CDS             4507..4809
                     /locus_tag="ROI21_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDX5113434.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            4856..5278
                     /locus_tag="ROI21_004612"
     CDS             4856..5278
                     /locus_tag="ROI21_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDX5113435.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            5275..5466
                     /locus_tag="ROI21_004613"
     CDS             5275..5466
                     /locus_tag="ROI21_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113436.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
BASE COUNT         1159 a         1542 c         1736 g         1249 t
ORIGIN      
        1 tatcgtcggt ctgattatta gtctgggacc acgatcccac tcgtgttgtc ggtctgatta
       61 tcggtctggg accacggtcc cacttgtatt gtcgatcaga ctatcagcgt gagactacga
      121 ttccatcaat gcctgtcaag ggcaagtatt gacatgtcgt cgtaacctgt agaacggagt
      181 aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc ttatccacaa
      241 cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc cggcacgtta
      301 accgggctgc atccgatgca agtgtgtcgc tgtcgccggc ctcctcaccc ggtcacgttt
      361 cgtcgttcct cctccacgcg ctgcggcttc ggggccgcac ctgcattcgt atgcggtcgc
      421 ccggttacag gtgcggcacg gcctgatgga ggccgcatgt gagaggagaa ttcccatgcc
      481 aaactggtgc tcgaatcgta tgcatttttc tggtgaaccg gcacagattg ctgagattaa
      541 acgactggcc agcggtgccg tcacaccgct ttatcgccgc gccacaaatg aaggtattca
      601 gctgtttctg gccggaagtg ccggacttct gcagaccact gaagatgtgc agtttgaacc
      661 gtgccccgga ctgacggatg ccggacgtgg tgttgtatcg ccggagaata tcgcgttcac
      721 ccgctggctg acacacctgc agaacggtgt actgctggat gagcaaaact gcctgatgct
      781 gcatgaactc tggctgcaga gtggtactgg ccagcgtcgc tgggaaggat taccggatga
      841 ggtcagggaa accatcaccg tgcatttcac cgcaaaaaga ggtgactggt gtggcttctg
      901 gagtaacgag gatgtatcgg tgtggtggaa ccgtctgtgt gacaatgtac tgccggaaaa
      961 aaccatgccg tttgacctgc tgacggttct gcccacccgc ctggatgttg aagtgaatgg
     1021 ctttaacggt ggtgttctga acggtgttcc ttctgcgtat cactggtata cggaacagta
     1081 tggcgtgaag tggcctgtgg ggtatgaggt gaatatcagc cgtcagggag aaaacttcat
     1141 tcaagtggat ttcgacacgc cgtggtgtca gccggaaagc aacgttgttg cagaattaag
     1201 ccgccgtttc ggctgcacgc tggagcactg gtacgccgaa cagggctgca atttctgtgg
     1261 ctggcagcga tatgagcgtg gagagcttgt tgatgtgctg tggggggaac tggaatggtc
     1321 ttccccgaca gatgatgatg agctgccgga agtcaccgcg ccggagtgga tagtcgacaa
     1381 ggtggcgcat tacgggggat gaagtatgac ggagacgggc gggcaaccgc ccgtttcttt
     1441 tccgacaaag gatgtcgccg tgctcctctt tttactgcgc cggctgacgc ggcgcggcag
     1501 gaacgctgcc tgtggtcagt gtcccgcgtc cggtgggcac gggacgggcg cttttactgc
     1561 tcccggctgg caccggtgac agtgtacgcc agcctgtcgc ccttttctga tgaacgccct
     1621 tcaagccgct ttcgcggcat attctcgccg tcaaaaagac gacggctgcg atattccacg
     1681 gtcggcctga cccgttaccg gacgcggtga acagcccaca ggcagcgggg aacgggcacc
     1741 acaggggggc cctcccggtg ccctctgtaa gagaaggagc gttgttatgt cccgttttgt
     1801 ccttggtaac tgcatcgatg ttatgacgcg tatccctgat aacgccattg atttcatatt
     1861 aactgacccg ccatatctcg tcggttttcg tgaccgttcc gggcgcacca tcgccggcga
     1921 taaaaccgat gaatggctgc aaccggcctg taatgaaatg taccgcgtac tcaaaaaaga
     1981 cgcgctgatg gtgagcttct acggctggaa ccgcgtcgat cgctttatgg ccgcctggaa
     2041 aaatgcggga ttcagcgttg ttggtcacct ggtcttcacc aaaaactaca catcgaaggc
     2101 cgcatatgtg ggctatcgcc acgaatgcgc ctacatcctg gcaaaaggcc gtccacgtct
     2161 gccacaaaac ccgctgccgg acgtgctggg ctggaaatat tctggcaatc gtcatcaccc
     2221 gacggaaaag cccgttacca gcctgcaacc gctgattgag agcttcacac acccgaacgc
     2281 aattgtgctg gacccgtttg caggcagcgg ctcaacctgc gtcgccgccc tccagtccgg
     2341 acgccggtat atcggtatcg agctgcttga gcagtatcac cgtgccgggc agcaacgcct
     2401 tgccgccgtg caacgggcca tgcagcaggg ggccgcgaat gatgactggt ttatgccgga
     2461 ggctgcgtaa atgaactatg ccggacacga aaaacttcgc gccgacgtgg cggaggtggc
     2521 caacaccatg tgtgaccttc gggccaggct gaacgatatg gagcatcgct gtcgctttga
     2581 ttccgatgtg cttgttgaac gcctgacacg ccagacgctg tatcgcgcca atcgcctgtt
     2641 tatggaggcg tataccgaaa ttctggaact ggattcctgc ttcaaagatt gagaaggaga
     2701 cgagaatgta cggaacatgc gaaacgctat gccgggagct ggcagcaaag tatccgggag
     2761 acgcgccgct gatgctggtt atctggtccc cggaagagat tcaggcactg gctgacggta
     2821 tggaaatttc tctgaccgat catgaaatca ggaccgtcct ggcgcgcatg gaggacatcc
     2881 cggaagacca gcggactgaa tccggtattt cttccgccgc ggtgatggag attatcagca
     2941 acgtgagcga aaaccgccag gtgaccgtcc ctgctgaact gctggcgtcc ctgattcaga
     3001 ccgctgaaca ggcactgtgg aaacgtgagt gggccgcccg tgataacggc ctcgccgtcc
     3061 cggaatgcgt tacccgccgt caggcggtgg ttaatcaggc ccgcaccctg ctgaaaaaca
     3121 acacacacga aaacaactga tgttatcgcc gcctgccctg cgggcggcga tacagggaga
     3181 acgcttaatc atgaacgaaa acagcacgct gaacgcactg atttgtcgtc acgcccgtaa
     3241 cctgctgctg gcgcagggct ggccggaaga gacggatgtt gaccagcgaa atccgaacta
     3301 tccgggctgg atcagcattt acgtgcggct ggatgcgccc cggctggcga cgttacttat
     3361 caaccgtcac ggcggcgtac tgccgccgct cctggcctcc gccattcaga gactgaccgg
     3421 aaccggggcg gaactggtac tgtccggcag tcagtggcag tcgctgccgg tacttccggc
     3481 agacggaacg caggtgtctt tcccgtatgc cggagaatgg ctgaccgaag acgaaatcag
     3541 ggctgttctt gatgcggtgc acgatgcggt acgaagcatc tgttaccagg tggcagaaga
     3601 tgcgcggcgt atccgtgcgg cgctgaccac caccggtcag acgttgctga cccgccagac
     3661 gcgccgcttt cgcctggtcg tgaaggaaag cgatcacccc tgctggctcg atgaagatga
     3721 cgaaaacctg cccgtggtgc tcgatgccat cgtgaaccgg ggagcacgtt tttcgtcggt
     3781 ggaaatgtat ctggtcagtg agtgtattga gcatatcctg tccagtgggc tggcctgcga
     3841 tgtgctgcgt ataccggatg aaccgccccg ccgctggttt gaccgtggtg ttctgcggga
     3901 ggtggtccgg gaagcccgga ccgaaatccg cagcatggcg gatgccctgg caaaaatccg
     3961 gaaatgatga cactggcggg gtaaatcccc gcctttttcc tgccggtccc cggacagagc
     4021 acaaaggaag tcgccgccgg ctccgcttta cccggccatg cagggcatgg ccttgtggtt
     4081 tttcagttat gtggcctcag cgtcgtgtgc gggctgtgcc gtgcctccat cttagccggg
     4141 ctggcgttga tgcaagggta cgcttcgccg ctgcggtcac ccggtccctc cttcccgtct
     4201 gccgtgattt tccggtcgtt cacccgctca gatttcgcgg tctcaccctg caactcccgt
     4261 cagccgtgtg cgtcaggctg cggcttccct tgcgcccctg catccccgcc tgttcgcccg
     4321 gcttttatgg aggcacggca ccgcccggtg tcgcggaata tgtgaacaac ggaggaaaac
     4381 atcatgcaat atgcgaaacc tgtcactctg aatgttgaag agtgcgaccg tctctccttt
     4441 ctgccgtatc tgtttggcaa cgattttctg tatgccgagg cgtatgtgta cgcgctggcg
     4501 caaaaaatga tgccggaata tcagggaggc ttctggcact tcatccgcct gccggacggt
     4561 ggcggttaca tgatgccgga tggcgaccgt ttccacatgg tgaacggtgc aaactggttt
     4621 gaccgtaccg tgagtgctga tgccgcaggc atcatcctga cctcccttgt gattaaccgc
     4681 cagttgtggc tgtaccacga cagcggggat gcaggactga cccagcttta ccggatgcgc
     4741 gatgcgcagt tgtggcgtca catcgaattt caccccgaat gcaacgcgat ttacgcggct
     4801 ctggactgat taacggacgg ggcggcaatg ccgcccctgt aaaggagacg agaacatgta
     4861 ctgtactgtt aaagaaatta tccgcgaagt gctggataca gacgtgccgg acagtgaatg
     4921 cgtttttgcc gtagtgctga cccgtgggga tgtgcgtcac atagcccagg actggagtct
     4981 gacagacgat gagctggaaa ccgtcatgca gcggctggac gatgcctttg aatatggtgc
     5041 ggatgtcagc gttgttcacg gcgttgttcg tgaactgatg gaagaaaagc gcgccagccg
     5101 tcaggtgaca gtcccggcgg tgatgctgga aaaagtgatg gcgctggcag gcagtgaaat
     5161 gaagcgcctg tatgccgtcg ggagtgagaa cgggggcgac ggtgacgcat tcgtcaggga
     5221 ggaacgcgaa gcaatggacg ttgtgttaca ggcgctggac ggggagacga tgtcatgaat
     5281 atcagcacag aaacccgcga aattctgcgt aattacaggg ccgtgattaa tgcgcggcgt
     5341 cgtgaaatgg ggcagaaacc gctcaccacc gcgcagattg ttgatgaaat ctgcgatttt
     5401 gtggcgaatc agcaggcggt tttcctgggc ggtcactaca tcttacaggg tagcagaaac
     5461 aggtgataca ggggcaggcg gtggaggccg cctgtcagtc cgcgcgaaaa cccctggcag
     5521 gctgacgcct gccggaaggg aagcgggcca cgctggcgcg tcgcccgtta cccttcccgt
     5581 ctgtgatgtg tctgactcag gagtgtttct ttcttatgtt atggaactgt tcctgatgtt
     5641 gttgttcctt tatctgctgc tctgattctg actactgcag tgttgt
//
LOCUS       DAPOZO010000181         5678 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPOZO010000181 DAPOZO010000000
VERSION     DAPOZO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.182"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            92..283
                     /gene="hemP"
                     /locus_tag="ROI21_004614"
     CDS             92..283
                     /gene="hemP"
                     /locus_tag="ROI21_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDX5113437.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(287..1723)
                     /locus_tag="ROI21_004615"
     CDS             complement(287..1723)
                     /locus_tag="ROI21_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDX5113438.1"
                     /translation="MTLSFVTRWRDELPATYTTLSPTPLNNARLIWHNAELANTLGIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVALSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRVRLQQDEVTDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1786..2499)
                     /gene="ydiV"
                     /locus_tag="ROI21_004616"
     CDS             complement(1786..2499)
                     /gene="ydiV"
                     /locus_tag="ROI21_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDX5113439.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPVNQITTLVQR"
     gene            complement(2746..3210)
                     /locus_tag="ROI21_004617"
     CDS             complement(2746..3210)
                     /locus_tag="ROI21_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDX5113440.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTKIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(3288..4037)
                     /gene="btuD"
                     /locus_tag="ROI21_004618"
     CDS             complement(3288..4037)
                     /gene="btuD"
                     /locus_tag="ROI21_004618"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDX5113441.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(4037..4588)
                     /gene="btuE"
                     /locus_tag="ROI21_004619"
     CDS             complement(4037..4588)
                     /gene="btuE"
                     /locus_tag="ROI21_004619"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDX5113442.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(4651..5631)
                     /gene="btuC"
                     /locus_tag="ROI21_004620"
     CDS             complement(4651..5631)
                     /gene="btuC"
                     /locus_tag="ROI21_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDX5113443.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWS
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
BASE COUNT         1490 a         1521 c         1315 g         1352 t
ORIGIN      
        1 cttgttgtct tctcaacaaa ttactgcttg ctctggcccg ccataatatt gataataaga
       61 atcattgtta tatcaattat tattaatctt tatgcgttat acggatagca gaaaactcac
      121 gcctgaaacg gatgccaatc acaagaccgc ttccccgcag cccattcggc gaattaccag
      181 ccagacactg ttaggtccgg atggcaaact gattatcgat catgacgggc aagaatatct
      241 gctccgtaaa actcaggctg gcaagctgct gttgaccaag tagcctttaa ctcgagcagc
      301 tgacttccag ccgtttaccc cagtcaggtg gacgactgac atagtcatca tctctgtcgc
      361 tgaaaggatt tcgcaacgcc tcatgcaggc ggtgcaattc cgtcatatca cccttttctg
      421 ccgcttcaat cgcccgttgc gccaaccagt tgcgcaacac cagagctggg ttaacgcttt
      481 gcatcagctg ctgacgctca ctatcggtaa cctcgtcttg ttgcaaacgt acccgataac
      541 gggcaaacca gtcatcaaat gccgcacgat cgataaactc atcacgtagc ggtgacgccg
      601 cgctgtgctg ctcggtcaga ctcagcatgc ggaatgtgcg ggtataatcg ctgcgctctc
      661 gcgccatcag actgaataat tcattcagta gcgcgttatc ctctttttgc tccgtcatga
      721 agcccagttt ctgccgcatc cgttgtccat aatgcgtcaa caaaacctgc tgatagctgt
      781 ccagggcctc attcagggca tctacggcaa caaatggtga caatgtctgc gccagacgct
      841 gtaaattcca caacgcgaca gcaggttgat tatcaaagct ataacgccct tgatgatccg
      901 agtgattaca aataaaaccg ggttcgtaat catcaagaaa accaaacggc ccgtaatcaa
      961 gcgtcagccc cagcagcgac atgttgtcgg tattcatcac cccatgagca aagccgaccg
     1021 tctgccattg ggcaattaac gatgcggtac gtgcgacaac atcggtaaac cagagacggt
     1081 atttgtcctc atcatctgca agatgtgacc agtaatgacg aatggcaaag tcagctaact
     1141 gacgaacctt ttccggctcg cggcgatagt aaaaatgttc gaaatgacca aagcgcagat
     1201 gacttagtgc cacacgcatc agcatcgcgc ctggctccac cgtttcccga tacactggcg
     1261 aatcgctggt gacgatactt aaagcgcggg tcgtcggaat gcccagataa tgcatcgcct
     1321 cactggcgag actttctcgt atcgtcgaac gtaaaaccgc ccgtccatca cccattcgcg
     1381 aataaggcgt caggccagca cctttcagat gccagtccat tgtagtgcca tcagcaagcc
     1441 gttgttcgcc gagtaaaatg ccgcgcccat cacccagttg gcccgcccag acgccgaact
     1501 gatgaccact gtaaacctgc gccagtggtg acataccagg cagtagggtt tcgccacccc
     1561 agacacctgc gccattttta aacagtgacg atggaatacc cagcgtgtta gccagttcgg
     1621 cattatgcca aatcagccgg gcattattta aaggcgtagg ggaaagtgtt gtataggttg
     1681 ctggcaattc atcgcgccag cgggtaacaa aagacagggt catagatcct cctgtttgat
     1741 agtgtagacg gttactctcg tcttaaacac cagcaaacag gagggttatc gctgaaccaa
     1801 cgtcgttatc tgatttacag gaacggcagg ccacaggcat ccctggagag catggaaatc
     1861 aaagggggta atctgtgcca ttatttctgg cgtatcaatg ccccccgcga tgatgcaatt
     1921 gcaacaaggg gaaatttgcg cctggatcgc acgaataaac ggttcgaagg agcgatgcgt
     1981 gatttgttgc tgaataaaac ttttatccag catgacgcgg gtgaaaagac catcaaaaac
     2041 cgctttcatt gtgctattgc ctgcgcccaa attacctaac acaaggggat acatttgcga
     2101 taaggacaaa agatcgcgat tatcttttcc ttcattgaga tgcgggtaat tctcattaat
     2161 caacagttca ataaacggat attttaatag ttcgcccgcg taattatccc tgtctaataa
     2221 taaagtagcc acttgtgggg ttaaattaag ccaggcaaag agtttatgtt gaataaaaaa
     2281 gtgttggcat gattttagca attccaactg ctcagaaaat aattgccaat gttgctcctc
     2341 ggtaagttgc gctataaccc gactcgtcgg gatacgaacc gtaccatctt cactggagaa
     2401 atgggtaatt aactcaacgc cgacaaggtc ttgctggtta tctcttatcg gcaggaaata
     2461 gcaatcagaa tgataaagat tctccaaaaa aatcttcatg gtaaccgtcc ctctcgaagg
     2521 atgtttttca gtatccggtt aaaagggatg aggccataag acataacaat tacagaagga
     2581 gtaactttca tttgttccat gttaaccact ttttcagggg tccttttatt agattatcct
     2641 gattataaac gaataatcca tttacggaat ttttgtctgt aaaatactac tgtattcagg
     2701 agtaaatgga cggagatgta tcatcgccgt ccatttttcg acatactaga ttcgtctcgc
     2761 ctgccagaaa tttttctgcc aatagacatt atcgagtgag gaacgcatca ctcccttgct
     2821 ggtagaggcg tggataaatt ggttgttggt atcgtaaata cctacatgca aaccattttg
     2881 tccggagccc gttttgaaaa agaccaggtc accaggcagc aactcgtctt tatcaatttt
     2941 cgtgccgata gaggcttgtt gtttggtttc acggggcagc tgcaaatcga aacgatcgcg
     3001 catcgtcaca accacaaacc ccgaacagtc cacaccgcgc cgcgtcatgc caccataacg
     3061 atacggcgtg ccatgccagc tctgtagctg gtcgttcaaa ccggcaataa cggtaatcga
     3121 atcagaaagt ctggcatttg gcggcggtgc tttatggtgg ctacacccgg ccagaaacag
     3181 tgctgtgatc aaaataaggc agaaacgcat tccgtacggt tcctctgttt tttattcttg
     3241 cattaattta gcgtcttaat tacccgattt tcaagatact aatgaaatca gatggtcgaa
     3301 atcagcattc ggtgaccttc gatatccaga cggcgaaaat tcatcccata ggcctgcgcc
     3361 agatttggcg gcgtgagcac ctcttctctg cgtccactgg ccagcatttt tccacctttt
     3421 agcaaccacg cccgatgcgc atgacgcaat gtgtggttga gatcgtgact gctcatcaca
     3481 atcgccagtc cttgctgaca cagcgcgctc agaattttgt ctaacgcact ttgttgcgca
     3541 acatcaagac tgttcatcgg ctcatcaaga agcagcaatt gacctgcggg attggcttgt
     3601 ggtgtgattt gcagcaccac cgcagcaaga cgtacgcgtt gccattcacc gccggaaagt
     3661 tgattggtgc tacgtccgag tttgtcatca agagccagcg cccctgcgac atcattcagt
     3721 agttcggtac gcgttttatc gtgctgatgc agtgtcaggt agtgccagac cggcattgca
     3781 aacggtggcg tctgttgttg tgaaagatag gcgcgatgca gcgcgagttt tgttgcggac
     3841 catgcttcca gtggttgccc cgcgaactga atgcttccct taccgctggt cattccggcc
     3901 attcgcgcca gtaaggtact cttacccgcg ccattcggcc ccaccagatg caggatctcc
     3961 cctgcccgaa cctcgccaga aagcggcccc aggcgggtag attccgcaac atcctgtaac
     4021 tgcatcacaa tagacattat tttgccaacg ccagtttaat gctttccatc acaatgggat
     4081 cttccggcgt catatccggg gaaaaacgct ggatgaccaa tccgtccctg ccaactaaaa
     4141 atttctcaaa attccataaa atatcatcag ggtacagcgg tgcacgacct ttgctgacca
     4201 tacgggcata gaatccgctc tcttccggcg cgactgcggt cggcgctgcg gcaatcaatt
     4261 tttgatacag cggatggcgt ccttcgccat taacttcaat cttactgaac atcgggaacg
     4321 tcacccccca tgtggtggta cagtaagttt taatctcttc atcgctgccc ggttcttgtt
     4381 ccagaaactg gttgcacggg aatcccagca ccacaaaacc tcgatcggcc caggctttct
     4441 gaatattctc caactgctca tattgcggcg ttaagccaca ctttgaggcg acattgacaa
     4501 tcaacagcac attaccggcg tacttctcca gcgtggtcac ttcaccgtcg atatctttca
     4561 ctacggtcgt cagaatggaa tcttgcatcg tttctcctgg gtgtggtcag taaaaatctt
     4621 agcttttaat catagaccgt ctttttgcgg ctaacgtcct gcttttaaca ataaccagat
     4681 aaacaccggc gcacccaacg ttgcggtgac cacgccaata ggcagctctg cggcagctaa
     4741 tgccaggcgc gctacaatat cggccagcag caatgcgctc gcccctgcca gcgcgcagcc
     4801 gggaagtaat acgcgatgat cggttaaacc acacagccgg agaatatgtg ggataaccag
     4861 accaataaag ccgatagcac ccgccagcgc cacactgacg ccaaccatcc agccggtcgc
     4921 tgccaccagc acattgcgcc agaaccacag gggtaaaccc agttgccgcg ccgagatctc
     4981 gccaagtgct aacatattca tcggcctgga ctgacaacag atccacaaca acacggggat
     5041 caatgccagc atcagccagc tttgccgcca gtctacgccg ccaaaaccgc ccatcatcca
     5101 gtacatcagc tgacgtaaat caacagaggt ggaaaagtag atagcccacg tcattagtgc
     5161 gctacagata atccctaatg caacgccagc cagcaataac cgactggtcg aaagatgacg
     5221 acgggcgaaa cgtaagagta ttaaagtgat gataagcgcg ccagcaatcg cacacagccc
     5281 tagtgcccag tttgggagtt gcccttgccc aagcaacacc gcggcgataa gccctacgcc
     5341 tgcgccgtta gagacgccaa gtagtccagg ttctgccaga ggattttcaa acaacgcctg
     5401 cattactgcg ccggatatag ccagcgccgc accaaccagc aatacagcca gcgtacgtgg
     5461 caggcgaatt tgccagacga acagttcgcc acgaggagta gaccagtcac cgggcgaaat
     5521 ccattgttca ccggcgcaaa ggcttaagag aagcgccagc agcatcaaaa ctgacaggca
     5581 taataaccag cgaatatttt gtcgctgttg ttggcgggca agtgtcagca tggtatccgt
     5641 tctgctgaag tgtcatggcg ttgattttac ggtgactc
//
LOCUS       DAPOZO010000182         5637 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPOZO010000182 DAPOZO010000000
VERSION     DAPOZO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.183"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(581..2962)
                     /locus_tag="ROI21_004621"
     CDS             complement(581..2962)
                     /locus_tag="ROI21_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HDX5113444.1"
                     /translation="MKTLKNWKLQNQSAHHIELLVDGQHSLCLYILEENMFRVLLKRK
                     GVLSLDRTWSIAPEKDVPWEGRHREDISGFSLPTWNMEQNDELLTITTSSLRVIIHKP
                     LWLEWHYKDNAGQWQELVNDRPTSAYLINAHGDGVAHYQSRRNDERFYGLGDKSGDLQ
                     RTGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITHRSDISFGLFYDNLSNSWLDLGNEI
                     DNYHTTYRRWQAEAGDIDYYLFTGRCVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMQFIRLCKEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPHPKMMSQ
                     EFHNAGIHLAANIKPCLLQDHPRYNEVAEQELFIRDSEYNVPERSSFWDDEGSHLDFT
                     NPQTVAWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGKEIAIKHIRPVMPL
                     LMCRASMEAQQKFAPNKRPYLISRSGCAGLQRYVQTWSGDNRTNWDTLRYNTRMGLGM
                     SLSGLYNIGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPEV
                     TPAIRSAIELRYRLMPYLYTLLWQAHADDEPILRPTFLDHEHDVQTFEECDDFMLGRD
                     ILVASVVEAGQRQRRVWLPDNKTGWYDFYNGEWFCGGQWITIDAPLEKLPLLVRAGAG
                     IPLSERITYVSEAEDNHRKLKLFPIKGTGKSTGLLFEDDGETWGYTEGNALWLEWELD
                     CTATTIELRINTHGDYRPAWETLKVIIPQGESRQLLINGIEAYEWNMNLSCND"
     gene            complement(2977..4278)
                     /locus_tag="ROI21_004622"
     CDS             complement(2977..4278)
                     /locus_tag="ROI21_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5113445.1"
                     /translation="MSQDINNNIAVSKHRRIIRQLRWWMLILFLMGVTVNYITRNSLG
                     ILAPELKDSLGITTEQYSWIVGGFQLAYTLFQPLCGWLIDVIGLKIGFMICASLWGIA
                     CLLHAGAGSWIQLALLRFFMGGAEASATPANAKIIGEWFPKSERPVAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGALAILWVFLWWACYNTPEEHPNLSKNELNFIRQ
                     DNEAPPVKMPFLTALKTVSKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLAKAYGMDLK
                     QIALFAWLPFLCADLGSIASGYLTKLYTRLFGLSRVNSVIASSVSGAFLMVSLAVIAF
                     IDNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRKKRT"
     gene            4540..5610
                     /locus_tag="ROI21_004623"
     CDS             4540..5610
                     /locus_tag="ROI21_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDX5113446.1"
                     /translation="MEKKLKIADIAMRTGLSSSTVSRVLAGKANTSYRAREKVLACAR
                     ELGVMDGMASGRMLLNNLVIFAPQRAFDERTDIFYFRVIQSISKALSHYEVRLRYCAL
                     DEFDSTPSKFLARMNEAETQAAILLGIDDPHIHDLAADFSKPCVMINCHDRRMRLPTV
                     APDHKNIGAFASHFLFEMGHRRVMNIMCLRRYTMELRLAGIKEAWERQNQNFKDDRDL
                     LTINSFSAKEAEEKVGAWLDMTEKSMLPTAFLASGDFIAAGIINALKKRNIRVPQDVS
                     VMSIDGFNLAAIEDVPLTAVHVPRDELGTEAVHMLQQRLVRPDATVGALLLYGKLVIR
                     ESVRRIRPGKEPTPIKGDGLYD"
BASE COUNT         1675 a         1209 c         1139 g         1614 t
ORIGIN      
        1 cggtaaatat ccataagtgt gggcttgcgg aataaatagt ttggttaaag ataaggtggt
       61 ttaatactgc tgagtatttt ataggagata tgatgttgtt ttataaacaa actaaaaaac
      121 atgaagttta attgataggg gacaatgaaa tctccttttt aaactcaatt gctatgttgg
      181 gtttatgggg ttgctataaa cggttttgaa taaacgcttt actattctca tgaaaacagt
      241 gttatgttgt tattttaaca accccgcatt gttttttgat gggatgggta ttaagacttt
      301 agagaaactt tataaataaa tatatgtatt caatagtggc gaaaataata acccatctta
      361 ttattacatt gtggaagtgc tcttttttat tatcgctcat gttttacctt tctgttaaaa
      421 catattcttt cggatatgta aaatggtaag agatagttaa tctacgtatg catgggggcg
      481 tttcattgca tggcgttata atctggcttg tatataaagg cactataagt tttactctta
      541 cagtgccttt atttggccat gatttaccct ctacaataaa ttaatcatta caggataggt
      601 tcatattcca ttcataagcc tcaatgccgt taatcagtag ttgacgactt tctccctgag
      661 gaattatcac tttcaatgtt tcccatgcag ggcgataatc tccatgagtg tttattctta
      721 actcaattgt agtggctgtg cagtccagct cccattcaag ccacagagca ttaccttcag
      781 tatatcccca agtttcgcca tcatcttcaa acagaagtcc ggtggattta cccgttcctt
      841 taattgggaa taatttcaat ttacgatgat tatcttccgc ttcactcacg tatgttatac
      901 gttcactaag aggaatacct gcacctgcac gtactaataa tggtagtttt tccagtggcg
      961 cgtcgattgt tatccattga ccaccacaga accattcgcc attgtaaaag tcgtaccatc
     1021 ctgttttgtt gtcgggtaac catactcgtc gttgtcgttg ccctgcttcc acgacacttg
     1081 caaccagaat atcgcgtccc agcataaaat catcacattc ttcaaatgtc tgaacatcgt
     1141 gctcatgatc aaggaaggtt ggacgtaata ttggctcatc atcggcatgt gcctgccaca
     1201 ataatgtata taaataaggc ataagacgat aacgtaactc aattgcacta cgaatcgcgg
     1261 gtgttacttc cggatacatc catggttcat tgacagtatg atcatcattc cacgagtgga
     1321 tcgtaaatcg ggggtgcata acaccgttct gaacccaacg aacaaataat tctggatcag
     1381 gcttatcacc agaaaaacca ccgacatcat gaccaatgtt atacaatcct gagaggctca
     1441 ttcccagccc catgcgggtg ttgtatcgaa gggtatccca gttagttcgg ttgtccccac
     1501 tccatgtctg aacataacgc tgcaatccgg cgcatccaga gcgggaaatc aaatatggtc
     1561 gtttgttcgg tgcaaatttt tgctgtgctt ccatagatgc tctgcacatt aaaagaggca
     1621 ttactggccg aatgtgttta atggcgattt ctttgccaaa accatgacag cgcgcttccc
     1681 catcccatac ttcaaactca ttgttatcat tccaggtgga atcaattccc atttcaagta
     1741 gttgtgtggt tacaccttcc tgccaccatg cgactgtttg tgggttcgta aagtccagat
     1801 gagaaccctc atcatcccag aagctggagc gttccgggac gttgtattca gagtcacgaa
     1861 taaagagctc ttgttctgct acttcgttat agcgaggatg atcctgcaat aaacatggtt
     1921 ttatattagc ggccaggtgt atccctgcat tatgaaactc ctgactcatc attttagggt
     1981 gtggaacttt gtcgtaattc cagttgaata cgtagcgttt accattaata gaagtataac
     2041 cagaagatag ctggaaagaa tcacatggaa tagcatgttc cttacaaaga cgaataaact
     2101 gcatcaattg attctgagca tccggtgcat ctgtatagtg catcgtggaa ccgctgtacc
     2161 caagactcca ttttggaccg aagagtgttt tccccgtcaa acgaacaaag gctttggtga
     2221 tatcaagtac acatctgcca gtgaaaagat aataatcaat atctcccgcc tcagcctgcc
     2281 aacggcggta agttgtatga taattatcta tttcattacc taaatccagc caactattgc
     2341 tgaggttatc ataaaataat ccaaagctaa tatcgctacg gtgagtaatt gtaaatggaa
     2401 tatgtttata aagagggtct gtacttactg cattatatcc cattgcgtcc aggttccgca
     2461 tttcatagcg ttttcctgtg cgttgcagat ccccggattt atcaccaagt ccataaaaac
     2521 gctcgtcatt acgtcgactt tgatagtgtg caacaccatc accatgagcg tttatcagat
     2581 aagcactggt agggcgatca ttaacaagtt cctgccactg accagcatta tccttataat
     2641 gccattcaag ccataaaggc ttgtgaataa ttacgcgtaa tgaactggtc gtgattgtca
     2701 gtaattcatc attctgctcc atgttccagg tcggcagtga aaaaccgcta atatcttcgc
     2761 gatgacgccc ttcccacggg acatcctttt ccggagcaat gctccaggtt cggtccagcg
     2821 acaaaacccc cttccgtttt aatagaaccc ggaacatatt ttcttcgagt atatacaggc
     2881 aaagagaatg ctgaccatca actaatagct ctatatgatg ggctgactga ttttgtagtt
     2941 tccagttttt taatgttttc atctccacat actccctcag gtgcgttttt tgcgacgttc
     3001 tgcaataaaa gtaaccagaa aaaaagcacc aataagatca aaaaatccca tggcgataaa
     3061 tagcgggtta aagcctattt tgtcagcagt aacgccaata atcagtgaga acaaaaagct
     3121 ggcaatccat gcggctgaac cacgcattcc attaacagtg gccatctgcc ccttatcaaa
     3181 agattcaacg accagggcac ttaacatgca ggaaattatc tgatgaccaa aaccaccaat
     3241 ggagataagt acaatagcaa tatatggatt gtcgataaaa gcaatcacag caagcgaaac
     3301 cattagaaat gctccgctta cagaggacgc aataacagaa tttactctgc ttaacccaaa
     3361 cagacgggta tagagttttg tcagatatcc gctggcaata cttcctaaat cagcgcaaag
     3421 aaatggtaac catgcaaaca gagctatttg tttaaggtcc atgccatatg cttttgcaag
     3481 atataatggc acccaaaagc tcattacagc ccatgctggc tctgccataa atgcagggat
     3541 agcgattcca taaaagcgtt tatttttact aactgttttt aacgcggtca gaaaaggcat
     3601 ttttaccgga ggagcttcgt tatcctgtcg gataaagttg agctcatttt tacttaagtt
     3661 tgggtgctct tctggtgtgt tataacacgc ccaccaaagg aaaacccaaa gaattgcaag
     3721 tgcacctgta aacataaatg caccctgcca accaaatgac gcatgtgcaa agtaaataat
     3781 tggtggtgca agcattgcac cgatagaaaa accgacaccc gcccagccag cagcaacagg
     3841 acgttcagat tttggaaacc actcacctat aatttttgca tttgcaggtg tcgcagaagc
     3901 ctcagcacct cccatgaaaa aacgaagcaa cgcaagctgt atccagcttc ctgctcctgc
     3961 atgcagaaga caagcaattc cccataatga agcacaaatc ataaatccaa tcttgagccc
     4021 aataacatca attagccatc cacaaagtgg ctgaaaaagg gtatatgcaa gctgaaatcc
     4081 accaacaatc caggagtatt gttcagttgt aattccaagg ctgtctttta actctggtgc
     4141 caaaatacct aatgagtttc tggtaatata attgacagtg acacccatta gaaaaaggat
     4201 taacatccac caccgtagtt gtcttattat tcgacggtgt ttgctgacag ctatattatt
     4261 atttatatcc tgactcataa tcaccccacc ggatgtaata aatgatcgag cgacaacgca
     4321 gctcggggtt gccacatccc actttacctt tcggcttaat acttaaaccg gaactactca
     4381 ttcacaataa aagagggggg cgatgaaaaa aagttggtat tgtgcacgtt ttttcataag
     4441 tagttcatat aaattctaaa aatagtcaga agtccttgca gtcaggctat tccatcccat
     4501 gagaattttt tcattttgaa attagagata gatcacaata tggaaaaaaa actaaagatt
     4561 gctgatattg ccatgcgaac aggcctttca tccagcaccg tgtcacgggt tttagcgggt
     4621 aaagctaata ccagttatcg tgcccgcgag aaagtattgg cgtgtgccag agagcttggg
     4681 gttatggatg gaatggcatc cggccgtatg ttgttaaaca accttgtcat ttttgctcct
     4741 caacgggctt ttgatgagcg aacagacatt ttttatttcc gcgtaatcca aagcatcagc
     4801 aaggcattat cgcattatga ggttcggttg cgttactgtg cccttgatga gttcgatagt
     4861 acaccttcaa aattcctggc gcgcatgaat gaggcagaaa cacaggctgc aatattgctt
     4921 ggtattgatg atcctcatat acatgatctt gcagcagatt tttctaagcc gtgcgtaatg
     4981 attaactgcc atgatcgacg tatgcgtctt cccacagttg ctccagatca caaaaacatt
     5041 ggtgcttttg cgtctcactt tctgtttgaa atggggcatc gcagagtaat gaacattatg
     5101 tgtttacgtc gatacacaat ggaactgcga cttgccggaa ttaaagaagc atgggagaga
     5161 cagaatcaga actttaaaga tgacagagat ttactcacta ttaatagttt cagcgccaaa
     5221 gaagccgaag aaaaagtcgg agcatggctg gatatgacag agaaaagtat gctaccgact
     5281 gcttttttgg caagtgggga cttcattgct gcaggtataa ttaacgcttt aaaaaaacgt
     5341 aatatacgag tgccacaaga tgtgtccgta atgagtattg atgggtttaa tctggctgca
     5401 atagaagatg tgccacttac ggcagtacat gtacctcgcg atgaactggg tactgaagcg
     5461 gtacatatgt tacaacaaag acttgtacgt cctgatgcaa ctgttggtgc attacttttg
     5521 tatgggaaac tggtaatacg agaatctgtt cgccgtatac ggccagggaa agagccaact
     5581 ccgattaaag gagatggact gtatgactga aaccacagcc tctgattctt ccggtca
//
LOCUS       DAPOZO010000183         5450 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPOZO010000183 DAPOZO010000000
VERSION     DAPOZO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.184"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(75..1358)
                     /gene="aroA"
                     /locus_tag="ROI21_004624"
     CDS             complement(75..1358)
                     /gene="aroA"
                     /locus_tag="ROI21_004624"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDX5113447.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTLLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQEHYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATVALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTAVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            complement(1429..2517)
                     /gene="serC"
                     /locus_tag="ROI21_004625"
     CDS             complement(1429..2517)
                     /gene="serC"
                     /locus_tag="ROI21_004625"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDX5113448.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2716..3408)
                     /locus_tag="ROI21_004626"
     CDS             complement(2716..3408)
                     /locus_tag="ROI21_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDX5113449.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3538..5298
                     /locus_tag="ROI21_004627"
     CDS             3538..5298
                     /locus_tag="ROI21_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HDX5113450.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1411 a         1381 c         1284 g         1374 t
ORIGIN      
        1 ccgaaaaaaa acgccggcaa tgtgccgacg tctttgaaaa gaaaggattg tctatgttat
       61 cgcccgttat tcattcagcc gggttgactt atccgcgcca attgctcgaa ataatctgga
      121 aaagttttag cggtacattt tggatcaaga atcgtcacag ccgtatctga taatgcgacc
      181 agcgagaaac acatcgccat ccggtgatca ttataagtgg cgatctcagc aaatttcagt
      241 ttttccggtg gcgtaatgcg aataaaatcg tgcccctctt cgacttccgc accgacttta
      301 cgaagttctg ttgccatcgc aaacaggcga tcggtctctt taacacgcca gttatagata
      361 ttgcgcagcg tggtggtgcc ttttgcaaat aacgccacgg tggcaatggt catcgccgca
      421 tcgggaatat ggttcatatc catatcaatg gcgttcagtt caccacgcgt gcaggaaata
      481 taatcatcgc cccagcaaat ggtcgccccc attttttcca gcacatcagc aaagcgaata
      541 tcaccctgca tactgttgcg tccaatgccg gtcactttta cagtgccgcc tctgattgct
      601 gctgctgcca ggaaataaga agccgaggat gcatcgcctt cgaccaaata agtacccgga
      661 gactgataag actgcccccc ttttacgaca aattgttgat agtgctgatt ttcaatttca
      721 acaccaaacg tcttcatcag attgagggtg atgtcgatat aaggtttaga aaccagatcg
      781 cctttaatac gaatcaccgt atcttccggc gcaagaggcg cagtcattaa tagtgcggtg
      841 aggaactggc tggaaacaga gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt
      901 aaacgcaatg gcggataatg ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc
      961 agcgcatcca ccagatgacc aatcgggcgc tctttcatac gcggttcacc ggtcagcaca
     1021 atatcattgc tacccagaca aagagctgcc gccagcgggc gcattgccgt tccggcgttt
     1081 ccgaggaaca actccagggc accttctgcg tgtaatggac cgccgttacc gataatttcg
     1141 caacgcgtac gatcagctga aagcgtatag cttaccccta acgctgttaa tgcattcagc
     1201 atatggcgca cgtcatcgct atccagcaga ttggttaata atgttttgcc atgtgctaat
     1261 gccgccagca ataaagcgcg gttagaaacg ctcttggaac cgggcagatt aatagtgcca
     1321 tcgacacgag cgatgggttg taacgttagg gattccatga actcaactct ctacaacaga
     1381 aataaaaacc ccacaggctg gctgtgggga ttatgcaaaa tttcgacatt agccgtgacg
     1441 gcgttcgaac tcaaccatga agtctgtcag tgctttaaca ccttccaacg gcatggcgtt
     1501 ataaatagac gcacgcattc cgccgaccac tcggtgacct ttcagcgcat gtaagcccgc
     1561 agcaaaagac tcttcaagga acaatttgtc aagcgcactg tccgccaact ggaacggcac
     1621 gttcatccgc gaacggttag ctttcgccac gtcattacgg tagaaatcgc tgttatcaat
     1681 caccccatac agcagttctg ctttttgctg attgatttta tccatttcag ctacaccgcc
     1741 gttcgctttc agccatttaa agaccagacc agacagatac caggcaaatg tcggcggcgt
     1801 gttaaacatg gagccgttat cgttgaggat ggaataatcc agaatcgacg gacacgcgat
     1861 attcgctttg cccagcaaat cttcacgaac gatgacgatt gtcaggcctg ccgggccgat
     1921 atttttctgc gcgccagcgt aaatcacgcc ataacggctg acgtcaatcg gacgggaaag
     1981 aatggttgaa gagaagtcgg cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc
     2041 gatggcgata ccgtcgatag tttcattcgg gcaataatgc atataagcag cattatcaga
     2101 gagttgccat tcacgcattg gtttaaccgc gcgcagacca tcaacagtca ctttggcgtc
     2161 aaagacatta ggcgtgcagt attttttcgc ttctttaatg gcacttgccg cccagtaacc
     2221 ggcatcaaca taatctgcag tggttttatc accgagaata ttcagcggaa cagcagcaaa
     2281 ctgaccgcga ccgccgccat ggcagaacaa taccttgtag ttggagggga cattaagaag
     2341 atcgcgaaaa tccttctcgg cttcctctgc aacctgaatg aactctttgc cacggtgact
     2401 cacttccatc accgacgtac caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt
     2461 aagcacctct gccggtagca ttgccggacc agaactaaaa ttgaagattt gagccatttc
     2521 ccctcaccac gttgcgttgc tattaacccg cggtcaatcg attgcgacca caggtaatac
     2581 attattcctg tggctatcgg ttttatcatt cagtgacaca catcgcaatg agtaaaaccg
     2641 ttgagggcaa aaatgtggct tgcatcacac aaaagaatct ctcggattat tagcaaaaag
     2701 ccgatgtttt tactgtcacg tcacccgttt tgcccgactt gccttcgtcc attctggatt
     2761 tgcacagcgc ggacataatt gtttttcccc cgcgttcata tgaatgattt cactgcaacg
     2821 aatgcaggca tagtccgccg actcgggtac cactttgtaa cgctgagtac agtcactggg
     2881 taaaataagt aagcccggtt tcaccttgtc atcttcaaag aaatagacac tgatttgccc
     2941 gttggtttcg agaatcgcca gacgtacttg ccccaactgc tctacaccgc gcaatcgtag
     3001 ctccataaag aactcaaatt ccgtcatgtt ggagttattg agttttgacc aggccagctc
     3061 gccatcttca ataatgacaa ccggcttgcc ttccagaaga tcttccagtt tttcactgtg
     3121 cgccatcaac cacattacca ggcggtataa caacgccaga gtgataaaga cgataagtac
     3181 cgggaccatc ggcacatcat cataaaacgc cacatctccc gccgctgacc ccagcgtcag
     3241 aatgattaac acttcaaaca gcgacatctg ccgcacaccg cgtcttccgg tcattttgag
     3301 gaacaaaaaa accagtacaa aggtataaag actacgtagt gccacttcgc caaggaaatc
     3361 aaaaggcact ttatcaaatg ccatacggtg gagatcgaac gccttcattt ttatgctctt
     3421 atcgtggttt atccactaaa agcatagtca acagttcatc ggcatggcgg cggtgatagc
     3481 gcctttgaga taaaacccgt attattgcgc gctttccgta cgactaaagt gattttcatg
     3541 acgcaaacat ttatccccgg caaagatgcc gcactggaag attccatcgc tcgcttccag
     3601 caaaaacttt cagacctcgg ctttcagatt gaagaggcct cctggctgaa tccggtgcct
     3661 aacgtctggt ctgtacatat tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa
     3721 ggcgcaacca aaaaagcggc gctggcttct gcactcggtg aatatttcga gcgtctctca
     3781 accaactact tttttgctga tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg
     3841 cattatccca acgaaaaatg gttcccactg agcgaaaatg acgatgtgcc agaagggctg
     3901 ctcgatgacc gtctgcgcgc attttacgat ccggagaatg aattgaccgg cagtatgctg
     3961 attgacctgc aatccggtaa cgaagatcgt ggtatttgcg gtctgccgtt tacgcgccag
     4021 tccgacaatc agaccgttta tattccgatg aatatcattg gtaacctgta cgtttctaac
     4081 ggtatgtccg ctggcaatac ccgcaacgaa gcacgcgttc aggggttgtc cgaagttttc
     4141 gaacgctacg tgaaaaaccg cattattgct gaaagcatca gcttgccgga aatcccggca
     4201 gacgtgctgg cgcgttaccc agcggtggtt gaagccatcg aaacgctgga agcagaaggt
     4261 ttcccaatct ttgcttatga tggttcgctt ggcggccagt atccggtgat ttgcgtggta
     4321 ctgtttaatc ctgctaacgg cacctgcttt gcctctttcg gtgcgcatcc tgattttggc
     4381 gtagcactgg aacgtaccgt gaccgagctg ctgcaaggtc gtggcctgaa agatttggat
     4441 gtgtttactc cgccaacctt cgatgatgaa gaagttgctg aacataccaa cctcgaaacg
     4501 cactttatcg attccagcgg tttaatctcc tgggacctgt tcaagcagga tgccgattat
     4561 ccgtttgtgg actggaattt ctccggcacc acggaggaag agtttgctac gctgatggct
     4621 atcttcaaca aagaagataa agaagtttat attgccgatt acgagcatct gggcgtttat
     4681 gcttgccgaa ttatcgtgcc tggcatgtcc gatatttatc cggctgaaga tctgtggctg
     4741 gcgaataaca gtatgggcag ccatttacgt gaaacgattc tttcgttacc aggcagcgag
     4801 tgggaaaaag aagattacct gaacctcatc gagcaactgg atgaagaagg ttttgatgac
     4861 tttacccgcg tgcgtgagct gttgggtctg gcgaccgggt cggataacgg ttggtacacc
     4921 ctgcgtatcg gtgaattaaa agccatgctg gcgctggctg gtggcgatct ggaacaggct
     4981 ctggtctgga ccgaatggac gatggagttt aactcatcag tatttagtcc ggaacgcgcc
     5041 aactattatc gctgcctgca aacgttgtta ttactggcgc aggaagaaga tcgccagccg
     5101 ctgcaatatt tgaatgcgtt tgttcgcatg tacggcgcag atgccgtgga agccgccagt
     5161 gcggcaatga gcggcgaagc ggcgttttat ggcttgcaac cagtagatag cgatctgcac
     5221 gcgtttgcag cacatcagtc gttgttgaag gcctacgaaa agctgcagcg cgccaaagca
     5281 gcattctggg caaaataaaa tcaaatagcc tacgcaatgt aggcttaatg attcgtctga
     5341 gttatattac ggggcgtttt tttaatgccc cgctttacat atatttgcat taataaaata
     5401 attgtaatta taaagttaaa tgtcggtaat ttgtatttaa taaatacgat
//
LOCUS       DAPOZO010000184         5408 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPOZO010000184 DAPOZO010000000
VERSION     DAPOZO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.185"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="ROI21_004628"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="ROI21_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDX5113451.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ROI21_004629"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ROI21_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDX5113452.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEEEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ROI21_004630"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ROI21_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDX5113453.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ROI21_004631"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ROI21_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDX5113454.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ROI21_004632"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ROI21_004632"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDX5113455.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ROI21_004633"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ROI21_004633"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDX5113456.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ROI21_004634"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ROI21_004634"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDX5113457.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQSGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ROI21_004635"
     CDS             complement(4598..5320)
                     /locus_tag="ROI21_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDX5113458.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
BASE COUNT         1349 a         1387 c         1307 g         1365 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tggtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcatat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcgatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt aatctcttct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gactcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacacca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagtagac gcaacagcgc agcaaaatcc gtcagccagg gtgaacgatg taatgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccgactga agatttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc atcgcttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat aaaaaaagac gctgcttaac
     4501 tcatggcctt ctgcaatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcgga
//
LOCUS       DAPOZO010000185         5279 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPOZO010000185 DAPOZO010000000
VERSION     DAPOZO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.186"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..738)
                     /gene="bamD"
                     /locus_tag="ROI21_004636"
     CDS             complement(1..738)
                     /gene="bamD"
                     /locus_tag="ROI21_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDX5113459.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAKYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            873..1853
                     /gene="rluD"
                     /locus_tag="ROI21_004637"
     CDS             873..1853
                     /gene="rluD"
                     /locus_tag="ROI21_004637"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDX5113460.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1850..2581
                     /gene="pgeF"
                     /locus_tag="ROI21_004638"
     CDS             1850..2581
                     /gene="pgeF"
                     /locus_tag="ROI21_004638"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDX5113461.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGHLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYLLARQRLANVGVEQIFGGNRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2711..>5279
                     /gene="clpB"
                     /locus_tag="ROI21_004639"
     CDS             2711..>5279
                     /gene="clpB"
                     /locus_tag="ROI21_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDX5113462.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     "
BASE COUNT         1294 a         1278 c         1443 g         1264 t
ORIGIN      
        1 ttatgtattg ctgctgtttg cggcgatgat tttcgctact ttttcagctt gcgcattcat
       61 ctgcatctga cggtatgcat tttccattag cggcagcgca tcacgcgtag cctgggtatc
      121 cgggtagtcg cgcaacatgc cttctacgcg gttaacgacg gcaacccatg cgccacgttc
      181 tgtatagtac ttggccacgg agtattcata tttcgccaga cgatctttca ggaataccag
      241 acgtttggtg gcatcggtgg tgtactgact gttcggatag ccgcgcacca gtttggaaaa
      301 gtcactaaac gcagctcgag catgttgagg atcgcgatcg ctacggtcaa cgccaaagaa
      361 cccttgcagc gcactgtcat ccagcgccat attggtcagg ccacgcatgt acatgacata
      421 atcgatattc ggatgggtcg ggttaaggcg aataaaacga tcgatggcag cctgcgctaa
      481 cggcaaatcg gcgtttttat agtaggcgta gatgagatcc agctgcacct gctgcgaata
      541 tggaccaaac ggatagcgat tatctaacgc ttccagttgc gttattgcct gtctccagtt
      601 accgtcctgc agcttttgtt gtgcagtcgc gtaaatttca tttggcggat tatcaggtac
      661 ttcttccttt gaccccgagc aacccgccaa aaacaggctt agtgtggcgg ctgccaccag
      721 atatttcatg cgcgtcatga cgttttgact ttcctcaaaa tgtaatacgg gagattctct
      781 gttcctgctc ccggttaaga ccagctacaa tagcacacta tattaaacgg caaagccgta
      841 aaaccccaac gataaacgaa gaagcagtat atatggcaca acgagtacag ctcactgcaa
      901 cggtgtccga aaaccaactc ggtcaacgct tagatcaggc tttggccgaa atgttcccgg
      961 attattcacg ttcgcgaata aaagaatgga tcctcgacca gcgcgtgctg gttaacggca
     1021 aagtttgtga taagccgaaa gaaaaagtat tgggtggcga gcaggtggcc atcaacgctg
     1081 agattgaaga agaagcgcgt tttgaaccgc aggatatccc gctggatatc gtctatgaag
     1141 atgaagatat catcgtcatt aataaaccac gcgacctggt ggtacatcct ggcgcgggta
     1201 acccggatgg tacggtattg aatgcgttgc ttcattacta tccgcccatt gccgatgtac
     1261 cgcgtgcggg catcgtccat cgtctggata aagacaccac cggcctgatg gttgtggcaa
     1321 aaaccgttcc ggctcagacg cgtttagtcg aatctttgca acggcgtgaa attactcgtg
     1381 agtatgaagc ggtggcaatt ggtcatatga ccgctggcgg tacagtagat gaaccaatca
     1441 gccgccaccc gaccaaacgt actcacatgg cggtgcatcc gatgggtaaa ccggcggtca
     1501 ctcactatcg catcatggaa cacttccgtg tgcacacgcg tctgcggttg cgtctggaaa
     1561 ccggacgtac gcaccagatc cgcgtgcata tggcccatat cactcatccg ctggtgggcg
     1621 atccggttta tggtggccgt ccgcgtccgc caaaaggtgc ttcggaagcg tttatctcca
     1681 cgctgcgtaa gtttgaccgc caggcgcttc atgcaaccat gctgcgtctt tatcacccga
     1741 tctccggcat cgaaatggaa tggcatgcgc ctattccaca agatatggtg gagctgattg
     1801 aggtgatgcg cgccgatttc gaagagcata aggatgaagt ggactggtta tgagtaagct
     1861 gattgtcccg cagtggccgc tgccaaaagg tgttgcggcc tgtagctcca ctcgtatcgg
     1921 cggcgtgagc ttgcccccgt atgactcgct caacctcggt gcccattgtg gcgataaccc
     1981 ggatcacgtt gaggagaatc gcaagcgact ttttgctgcg ggccatttgc cttcaaaacc
     2041 ggtctggctt gagcaggtac acggcaaaga tgtgcttaag cttactggcg aaccttatgc
     2101 ctcaaaacga gcagatgcct cttatagcaa cacgccgggc acggtttgcg cagtgatgac
     2161 tgccgactgc ctccccgtgc tgttttgcaa tcgagcggga acggaagtcg ccgccgctca
     2221 tgctggctgg cgtggactgt gcgcaggcgt gctggaagag acggtttcct gttttgctga
     2281 taatccggaa aatattctcg cctggttagg gccggcaatt ggtccacgcg cgtttgaagt
     2341 gggggcggag gttcgcgagg cgtttatggc agtagacgcc aaagcgagta cagctttcat
     2401 tcagcatggt gataagtatc tggcggatat ttatctcctt gcccggcagc gtctggcgaa
     2461 cgtcggtgtc gaacaaattt tcggcggcaa ccgttgtaca tatacggaaa atgagacttt
     2521 cttctcttat cgtcgcgaca agaccactgg tcgtatggca agtttcattt ggctgatata
     2581 acctaaagaa tcaagacgat ccggtacgcg tgattttctt ttcacattaa tctggtcaat
     2641 aaccttgaat aattgaggga tgacctcatt taatctccag tagcaacttt gatccgttat
     2701 gggaggagtt atgcgtctgg atcgtcttac taataaattc cagcttgctc ttgccgatgc
     2761 ccaatcactt gcactcgggc acgacaacca atttatcgaa ccacttcatt taatgagcgc
     2821 cctgctgaat caggaagggg gttcggttag tcctttatta acatccgctg gcataaatgc
     2881 tggccagttg cgcacagata tcaatcaggc attaaatcgt ttaccgcagg ttgaaggtac
     2941 tggtggtgat gtccagccat cacaggatct ggtgcgcgtt cttaatcttt gcgacaagct
     3001 ggcgcaaaaa cgtggtgata actttatctc gtcagaactg ttcgttctgg cggcacttga
     3061 gtctcgcggc acgctggccg acatcctgaa agcagcaggg gcgaccaccg ccaacattac
     3121 tcaagcgatt gaacaaatgc gtggaggtga aagcgtgaac gatcaaggtg ctgaagacca
     3181 acgtcaggct ttgaaaaaat ataccatcga ccttaccgaa cgagccgaac agggcaaact
     3241 cgatccggtg attggtcgtg atgaagaaat tcgccgtacc attcaggtgc tgcaacgtcg
     3301 tactaaaaat aacccagtac tgattggtga acccggcgtc ggtaaaactg ccatcgttga
     3361 aggtctggcg cagcgtatta tcaacggcga agtgccggaa gggttgaaag gccgccgcgt
     3421 actggcgctg gatatgggcg cgctggtggc tggggcgaaa tatcgcggtg agtttgaaga
     3481 acgtttaaaa ggcgtgctta acgatcttgc caaacaggaa ggcaacgtca tcctgtttat
     3541 cgacgaatta cataccatgg tcggcgcggg taaagccgat ggcgcaatgg acgccggaaa
     3601 catgctgaaa ccggcgctgg cgcgtggtga attgcactgc gtaggtgcca cgacgcttga
     3661 cgaatatcgc cagtacatcg aaaaagatgc tgcgctggaa cgtcgtttcc agaaagtgtt
     3721 tgttgccgag ccttctgttg aagacaccat tgctattctg cgtggcttaa aagaacgtta
     3781 cgaactgcac caccatgtgc aaattactga cccggcaatt gttgcagcgg cgacgttgtc
     3841 tcatcgctac attgctgacc gtcagctgcc ggataaagcc atcgacctga tcgatgaagc
     3901 agcatccagc attcgtatgc agattgactc aaaaccagaa gaactcgacc gactcgatcg
     3961 tcgtattatc cagctcaaac tggaacaaca ggcgttaatg aaagagtctg atgaagccag
     4021 taaaaaacgc ctggatatgc tcaacgaaga actgagcgac aaagaacgtc agtactccga
     4081 gttagaagaa gagtggaaag cagagaaagc atcgctttct ggtacacaga ccattaaagc
     4141 ggaactggaa caggcgaaaa tcgctattga acaggctcgc cgtgtgggtg acctggcgcg
     4201 gatgtctgaa ctgcaatacg gcaaaatccc ggaactggaa aagcaactgg aagccgcaac
     4261 gcagctcgaa ggcaaaacta tgcgtctgtt gcgtaataaa gtgaccgacg ccgaaattgc
     4321 tgaagtgctg gcgcgttgga cggggattcc ggtttctcgc atgatggaaa gcgagcgcga
     4381 aaaactgctg cgtatggagc aagagctgca ccatcgcgtt attggtcaga acgaagcggt
     4441 tgatgcggta tctaacgcta ttcgtcgtag ccgtgcgggg ctggcggatc caaatcgccc
     4501 gattggttca ttcctgttcc tcggcccaac gggtgtgggg aaaactgagc tttgtaaggc
     4561 gctggcgaac tttatgtttg atagcgacga ggcgatggtc cgtatcgata tgtccgagtt
     4621 tatggagaaa cattcggtgt ctcgtctggt tggtgcgcct ccgggatatg ttggttatga
     4681 agaaggtggc tacctgaccg aagctgtgcg tcgtcgtccg tattccgtta tcctgctgga
     4741 tgaagtggaa aaagcgcatc cggatgtctt caacattctg ttgcaggtac tggatgatgg
     4801 gcgtctgact gacgggcagg ggagaacggt cgacttccgt aatacggtcg tcattatgac
     4861 ctctaacctc ggttccgatc tgattcagga acgcttcggt gaactggatt atgcgcacat
     4921 gaaagagctg gtgctcggtg tggttagcca taacttccgt ccggaattca ttaaccgtat
     4981 cgatgaagtg gtggtcttcc atccgctggg tgaacagcac attgcgtcga ttgcgcagat
     5041 tcagttgaaa cgtctgtata aacgtctgga agaacgtggt tatgaaatcc acatttctga
     5101 cgaggcgctg aaactgctga gcgagaacgg ttacgatccg gtctatggcg cacgtcctct
     5161 gaaacgtgca attcagcagc agattgaaaa cccgctggca cagcaaatac tgtctggtga
     5221 attggttccg ggtaaagtga ttcgcctgga agttaatgaa gaccggattg tcgccgtcc
//
LOCUS       DAPOZO010000186         5134 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPOZO010000186 DAPOZO010000000
VERSION     DAPOZO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.187"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..428
                     /locus_tag="ROI21_004640"
     CDS             <1..428
                     /locus_tag="ROI21_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113463.1"
                     /translation="LSLFGIVGLTIFLKNIKRRCATSGRTVRVIDIENKNNESISYLF
                     TYIIPFVFQDLSTLTNVIPIAILLTVTALIYINSSMILINPTISINYTLYQVTYLDLE
                     SDKKRTGMVLTKSKYLEEDDLLDVEDVGPKLFYAESHKE"
     gene            425..1453
                     /locus_tag="ROI21_004641"
     CDS             425..1453
                     /locus_tag="ROI21_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HDX5113464.1"
                     /translation="MKMLKLEELLEYAEQLKDDDAAKISLYFITRHLKAGMSRTARVV
                     DKFDFKIIKAPIAPDIAKFFKYTLSNQIISHASKDDIVMKKYTVIDDDIDNKIYAYAM
                     NNAISFSKVINNDIKNDKPVVLTSLAEVQNDLWAYCIKVQKGADVTYSFRKISRGKVT
                     TNEPQNMTQRVFALFDKTDKELRSFDGSAVNFDDKIDCIYIKDQFYVFHKKSFEAIVG
                     LEVEFTEAAQKTLNTIKELDLIEGLDVIEQAILHKPSLRKILTHIAEKGNHTVLEKND
                     VQAMNDVLKMFQNEEFKTNEHGKLVIEDERQGRNFLKLLNDYYKQGMTTKKYYGTDSG
                     NVINPIKA"
     gene            1937..3250
                     /locus_tag="ROI21_004642"
     CDS             1937..3250
                     /locus_tag="ROI21_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDX5113465.1"
                     /translation="MIKETVTMSHKELHRLQIIQEQAAARIGISIRQVKRLVQRYRNE
                     GPSGLVSHRRGKRPNNSFSTEFRATVISLLKGRYADFGPTFACEKLREIHGLSLSVET
                     LRKWMIEEGLWRERRRKIARIYQRRMRRPSYGELIQIDGSPHDWFENRGPRCTLIVFI
                     DDATSALMALRFVPAETTRAYMETLRGYLNDHGVPLALYSDRHSIFRVNNPEREGELT
                     QFTRAIKTLGIEPIHANSPQAKGRVERANQTLQDRLVKEMRLQNISDIETANAWLPTF
                     IEAYNNRFATSPRTTDNAHLDVHHSEEELGYIFSLQAKRVLSKNLTFQYKSSAFQVRS
                     EGRGYRLRHSVVTVCENFDGEINVLYDGKALGWEKYVDGPEPIPLDDEKSVHERVDNA
                     RIDLRSKYYVKPKADHPWLTRRTQSHQQVKPPKLPKKKPDPDKKD"
     gene            complement(3687..4019)
                     /locus_tag="ROI21_004643"
     CDS             complement(3687..4019)
                     /locus_tag="ROI21_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein FlxA"
                     /protein_id="HDX5113466.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(4222..4527)
                     /locus_tag="ROI21_004644"
     CDS             complement(4222..4527)
                     /locus_tag="ROI21_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113467.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            4552..4791
                     /gene="relB"
                     /locus_tag="ROI21_004645"
     CDS             4552..4791
                     /gene="relB"
                     /locus_tag="ROI21_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HDX5113468.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            4791..5078
                     /gene="relE"
                     /locus_tag="ROI21_004646"
     CDS             4791..5078
                     /gene="relE"
                     /locus_tag="ROI21_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HDX5113469.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT         1539 a          971 c         1144 g         1480 t
ORIGIN      
        1 tattatcatt atttggtatt gtaggattaa caattttttt aaaaaatata aaacgtcgat
       61 gcgccactag tggtagaact gtaagagtta tagatattga aaataaaaac aatgaatcaa
      121 taagctatct ttttacatat ataattccgt ttgtatttca ggatctatcc actttaacta
      181 atgttatacc tatagcaata ttgttaacgg taacggcttt aatatatatt aattcaagca
      241 tgatacttat aaatcctaca ataagtatta attatacttt atatcaagtt acatatttgg
      301 atttagaaag cgacaaaaaa cgcactggta tggttttgac taaatcaaag tatttggagg
      361 aagatgatct tcttgatgtt gaggacgtgg gcccaaaact tttttatgct gaatcccata
      421 aggaatgaaa atgttaaagc ttgaagaatt attagagtat gctgagcaac ttaaggatga
      481 tgatgcggcg aaaatatcgt tgtatttcat tactagacat cttaaagcgg gtatgagtag
      541 aaccgcaaga gttgttgata aattcgattt taaaatcatt aaagctccaa tcgctccaga
      601 tattgctaaa ttttttaagt atactttatc aaaccagatt atttctcatg cttcgaaaga
      661 tgatattgtg atgaaaaagt atactgttat tgatgatgat attgataata aaatttatgc
      721 ctatgctatg aataacgcga tatcgttttc aaaagttatt aataacgata taaagaatga
      781 taaaccagtt gtacttacct ctcttgctga ggttcagaat gatttgtggg cctattgtat
      841 taaagtccaa aagggggctg acgtcactta ttctttccga aaaatcagca gggggaaagt
      901 cacaacaaat gaaccacaaa atatgactca gcgcgtattc gctttgtttg ataaaacaga
      961 taaagagtta agatcatttg atggcagcgc agtcaatttt gatgacaaaa tcgattgtat
     1021 ttatataaaa gatcaatttt atgtatttca taagaaaagt tttgaggcta ttgtaggctt
     1081 ggaggttgag tttacagaag ctgcacaaaa aacattaaat acaattaaag aacttgatct
     1141 tattgaaggt ttagatgtta ttgaacaagc tattcttcat aagccatcac tgagaaaaat
     1201 tctcactcat atcgctgaga aaggtaacca tacggttctg gaaaagaatg atgttcaagc
     1261 tatgaatgat gtgctaaaaa tgtttcagaa cgaagagttt aagaccaatg agcatggtaa
     1321 attagtcata gaagatgaga ggcaaggaag aaactttctc aaactgttaa atgattatta
     1381 taaacaaggc atgactacaa aaaaatacta tggtacggat agcggaaatg tgattaaccc
     1441 tattaaggcg taaagcatat actatatctg gaactgaaat accagccgcc atcagaaaga
     1501 actgatgggg cttacaaata gtaagtaaat ataaaatgtt aaggtatttt tcatgcaaaa
     1561 atgaaaatca ttggcacaaa ttagaaacta agtaaattgc taatactaat aacttattat
     1621 tctacttact atttgcatgg agttgttgtg aattgctgga aaggtgggta atgtttttgt
     1681 agaatcacgg cgggtgcttg aggctatctg cctcgggcac gaacaccaaa ggcagataga
     1741 gaaaagcccc agttaacatt acgcgtcctg caagacgctt aacattaatc tgaggccaat
     1801 ttcatgctag acacatgtag gttagcctct tacgtgccga aaggcacgga gaagcaggct
     1861 attgttaaca ccaagctgta atgtcccctt tgaaccattc taaaatgtcc ccagacaatt
     1921 ctctggggga tttttcatga tcaaagagac tgttacgatg agtcataagg aactccaccg
     1981 acttcagatt attcaggaac aagctgcggc acgcattggc atttctattc ggcaggttaa
     2041 acgtctggtg caacggtata gaaatgaagg gccttctggt ctggtttccc accgacgtgg
     2101 aaagcgtcct aataattcct tttctactga attcagagca acagtaattt cactcctcaa
     2161 aggccgttac gctgattttg gacctacgtt tgcgtgcgaa aaattgcgcg agatacacgg
     2221 tttatcttta tccgttgaaa ctctcagaaa gtggatgata gaagaggggt tatggcgtga
     2281 acgccgtcgt aaaattgccc gtatatatca acgccgcatg cgacgaccat cttacggtga
     2341 actgatccag attgatggct cacctcatga ctggtttgaa aatcgaggcc ccagatgtac
     2401 actgatcgtt ttcattgatg atgccaccag tgcgttgatg gcgttgcgtt ttgtgcctgc
     2461 tgaaacaacc cgggcttaca tggaaaccct ccggggttac cttaatgatc atggcgtacc
     2521 gctcgctctc tactctgata gacacagtat attcagggta aataacccag agcgggaagg
     2581 tgagctgacc cagttcactc gtgcgataaa gacactgggc atcgagccaa tccatgccaa
     2641 cagcccgcag gcaaaagggc gggtagagcg cgccaatcag acactacagg acaggctggt
     2701 caaagaaatg cggcttcaga atatcagtga tattgaaaca gcaaatgcat ggttgccgac
     2761 ctttattgaa gcctataaca accggttcgc tacgtcgcct cgtactactg ataatgctca
     2821 tcttgatgtg caccattctg aagaggaact gggttatatc ttcagcctac aggcgaagcg
     2881 cgttctgtct aaaaatctca ctttccagta caaaagcagt gcgtttcagg tacgcagtga
     2941 gggccgggga tatcgactta ggcattcggt tgttactgta tgcgagaact ttgacggtga
     3001 aattaacgtt ctgtatgacg ggaaagcgct gggctgggaa aagtatgttg atggcccgga
     3061 gcctatacca ctggatgatg aaaagagtgt ccatgaacga gtggataatg cccgtattga
     3121 tttacgctca aaatactatg ttaaacctaa agctgaccat ccctggctta cgcgccgaac
     3181 gcaaagtcat cagcaagtta agcccccgaa gttacctaaa aagaagcctg atcccgataa
     3241 aaaagattga aaccaagatc gattcggttg agtgcatatc cattcatagg gtagattctt
     3301 aagtcgcgtt tctggtgttc attttcgggt ggtttgttac ttgttttacc ggggatatgc
     3361 cagaaacgcg ctgagtcagt ctgggcggtg cgcgtaatga ggcgttatgg taaatagcct
     3421 atgctaatgt ccgctaagag caagaagcgg aagttggcag ttttgtggac tgtccccaca
     3481 aaagtgacta cagaaatagt tgcaattcat aattgatcat gggttgtcag ttaaactcgt
     3541 ggcgatttaa atagactaat tgggagtgcg tccattactt atatcttgta atgttaacta
     3601 tcagaaatga tacaaagata atatgtcttt aaagaaaagg ctgatggcga aaagtggccc
     3661 gatgagggcc acaatacggc tgtcacttag acgtaaatat caatggtgcc agcggtattt
     3721 gtatcgtctt ttttctcttc ttttttatca ggctgaactg tcgcgtcttc attctttttc
     3781 tctgcctgct gccttaacaa ctgctccagt tgagcccaga ggctttcaat ttgcttctgt
     3841 accaatgcag ccatttcttt tttctgctgt gtcgtcatcc cctcttccga tgagattttc
     3901 ccaagctttt cagtcagcac ctgaatttgt cttgtgattt tggctatttc tgatgttcct
     3961 tccggggcgg agttgtttga aataacggtt gaggtatttc cctgaattgt gacagacata
     4021 gatttctcct tttaaaaaag cactatcggc atgcacaaaa aaatctttaa tcgtatttct
     4081 tgtgtcatta attgtttgat gttcagattg ttttcctcgc gggctggcgc gcctcagaaa
     4141 gtaaagcttg ttgacagggg taaacgttcg gcaataattt tctgccgcat gcgggtgttg
     4201 cataaaacgt gttacgttcc tttatcgaca ggtcaggtca ccgctcaccc gccgacgaga
     4261 aagcaacact gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag
     4321 cctgaccgtc acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa
     4381 aacgagctga cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg
     4441 gacagtgatc accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat
     4501 gttgtagtgc gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc
     4561 attaacctgc gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg
     4621 ggtgtaactc cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc
     4681 ttgccgttca aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa
     4741 gaacggcttc gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt
     4801 ttctggattt tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg
     4861 aacagttgaa aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc
     4921 tccgtggtat gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat
     4981 accaggttat agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct
     5041 cggaagtata tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt
     5101 tcgcaccgaa ggtgacactt ctgctttgcg ttga
//
LOCUS       DAPOZO010000187         5001 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPOZO010000187 DAPOZO010000000
VERSION     DAPOZO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.188"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            229..768
                     /locus_tag="ROI21_004647"
     CDS             229..768
                     /locus_tag="ROI21_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDX5113470.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQSEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGEDYGFSDDIPF"
     gene            831..1064
                     /locus_tag="ROI21_004648"
     CDS             831..1064
                     /locus_tag="ROI21_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDX5113471.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1130..3088
                     /locus_tag="ROI21_004649"
     CDS             1130..3088
                     /locus_tag="ROI21_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDX5113472.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVILQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWGWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVGGISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGVAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NCWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3143..3577
                     /gene="psiB"
                     /locus_tag="ROI21_004650"
     CDS             3143..3577
                     /gene="psiB"
                     /locus_tag="ROI21_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDX5113473.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3574..4293
                     /locus_tag="ROI21_004651"
     CDS             3574..4293
                     /locus_tag="ROI21_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDX5113474.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            complement(4305..4493)
                     /locus_tag="ROI21_004652"
     CDS             complement(4305..4493)
                     /locus_tag="ROI21_004652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HDX5113475.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            4573..4731
                     /locus_tag="ROI21_004653"
     CDS             4573..4731
                     /locus_tag="ROI21_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDX5113476.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
BASE COUNT         1129 a         1296 c         1568 g         1008 t
ORIGIN      
        1 acatcccgca aaactttttg cccagaagca ggcaaaaact tttgcggtct gcccccggac
       61 agtcgtttcc gtccgtggca gtgatgcccc atgcggcgaa ggttctcaaa aaggtgatgg
      121 tgaataccgg agggaacaaa aggcaggtcc ccgcaaaacg tcaaaaccgc ggctcccgaa
      181 actcaaaaga tgatgggggc tgaaaacatc aacagaagga ggcacatcat ggcagttcgt
      241 ggcattaaca aggtcattct cgtaggacgc ctgggaaaag atccggaagt ccgttacatc
      301 cccaacgggg gcgcagtggc aaacctgcag gtcgccacgt cggaaacctg gcgtgacaaa
      361 cagacagggg agatgcggga gcagacagaa tggcatcgcg tggtgctgtt cggcaagctc
      421 gcggaagtgg ctggcgaata tctgcgcaag ggcgcgcagg tctacatcga aggtcagctt
      481 cgcacccgta gctgggaaga taacggtatc acccgttacg tcaccgaaat tcttgttaag
      541 accacaggca ccatgcagat gctgggacgt gccgcaggtg ctcagactca gccggaagag
      601 gggcaacagt tcagcggtca gcctcagccg gaaccacagt cggaggccgg tacgaaaaaa
      661 ggtggcgcaa aaacgaaagg ccgtggacgt aaggccgcgc agcccgagcc tcagccgcaa
      721 ccgccggagg gggaggatta cgggttttca gacgatatcc cgttctgatc gggctgactg
      781 tgataaccgc cccgccccgt tctgtgcggg gcatcaccgg agagatgaga atgagcgaat
      841 atttcagaat acttcagggg ctgcctgacg gtccttttac ccgcaaacat gccgaagctg
      901 ttgccgcaca gtaccggaac gtctttatcg aggatgacca cggcgaacag tttcgcctgg
      961 ttgtccgtaa taacggtgca atggtctggc gtacctggaa ttttgaggac ggtgccgggt
     1021 actggatgaa ccatgtcatc agggatttcg ggattcttaa gtaagagagg tgccggacgg
     1081 ggacacgtcc ggcagacaat cattattacc gttttgggag gtactaacta tgtcagttac
     1141 agagtctaag gcaaaaacgg agcgtaaatc cagccgtaaa cctgcaaaaa cgcaggaaac
     1201 agtcctgtct gccctgctgg cgcagacgga ggaagtgagc gtgccgctgg cctcactgat
     1261 taaatcaccg ctgaatgtgc gcacggtgcc gtattctgtg gagtccgtca gcgaactggc
     1321 tgagtccatt aagggcgttg gcctgttgca gaatctggtc gtgcataccc tgcctggtga
     1381 ccgttacggt gtcgccgcag gtggtcgccg actggctgca ctcaacatgc tggcagagcg
     1441 caacatcctt ccggctgact ggcctgtacg cgtgaaggtt attctgcagg aactggcgac
     1501 tgccgcatca atgactgaaa acggtcatcg tcgggatatg caccctgccg aacagattgc
     1561 cggattccgc gcaatggcgc aggaaggcaa aacacctgcg cagactggcg acctgctggg
     1621 ctattcaccc cgccacgtcc agcgaatgct gaaactggca gaccttgcac ccgtcatcct
     1681 tgatgcgctg gcagaagacc gcatcaccac cgaacactgt caggcgctgg cgctggagaa
     1741 cgacaccgcg cgtcaggtgc aggtgtttga agccgcctgc cagtcaggat ggggcggtaa
     1801 accggatgtg cgggttatcc gcaacctgat taccgaaagt gaagtggcgg tggctgggaa
     1861 cagtaaattc cgcttcgtgg gggctgatgc cttctcgcca gacgaactgc gcaccgattt
     1921 gttcagcgac gacgagggtg gctatgtgga ctgcgtggcg ctcgatgccg ccctgctgga
     1981 aaaactccag gctgtcgcag agttccttcg ggaagccgaa ggctggggat ggtgtgccgg
     2041 acgcatggag cctgtaggtg agtgccgtga ggatgccgga acataccgct gtctgccgga
     2101 gccggaagcg gtgctgacgg aggcggaaga agaacgtctg aacgaactga tgacgcgtta
     2161 cgacgcgctg gaaaaccagt gtgaggaatc tgacctgctg gaagcagaaa tgaagctgat
     2221 gcgctgcatg gcgaaggtca gagcgtggac gccggagatg cgtgccggag gtggagtggt
     2281 ggtgtcctgg cgttatggca acgtgtgtgt ccagcgaggt gtgcagttgc gcagtgagga
     2341 tgacgtggct gacgacgctg accgcacgga acaggtgcag gagaaagcgt cagtgggggg
     2401 aatcagtctg ccactgctga cgaaaatgtc ttccgaacgc acgctggcag tccaggcggc
     2461 gcttatgcag caaccggaca aatctctggc actgctggca tggacgctct gcctgaatgt
     2521 gtttggcagc ggagcgtaca gtaaaccggc acaaatcagc ctggaatgta aacattattc
     2581 gctgaccagc gatgcgccgt cagggaagga aggtgtcgca ttcatggcgc tgatggcaga
     2641 aaaagcccgt cttgctgccc tgttaccgga gggatggtca cgagacatga cgacattcct
     2701 gtcactcagt caggaggtgc tgttgtccct gctcagtttc tgcaccgcat gcagccttaa
     2761 cggtgtccag acccgtgagt gtggtcacac gtcacgcagt ccgcttgact cgctggaaac
     2821 cgctatcgga ttccacatgc gcgactggtg gcagccgaca aaagcaaact tcttcggaca
     2881 cctgaaaaag ccgcagatta tcgcagccct gaatgaggca ggactgtccg gtgctgcacg
     2941 ggatgcagag aagatgaaga aaggtgatgc ggctgaacat gcagagcacc atatgaaaga
     3001 caactgctgg gtgcctggct ggatgtgtgc accacatcca cagacggata ccactgaacg
     3061 caccgataac ctggctgatg ccgcctgata aacaaccaca ccgccccgcc ggagacgggg
     3121 cggcagcaag ggagataccg tgatgaaaac tgaactgacc ctgaatgtat tacagaccat
     3181 gagcgcacag gaatatgaag atatccgggc agccggaagt gatgaacgtc gtgagctgac
     3241 acacgccgtg atgcgggaac tggatgcgcc ggacaactgg atgatgaatg gcgagtatgg
     3301 cagtgagttc gggggctttt tccccgtcca ggttcgtttc acgccagccc acgaacgttt
     3361 ccacctggcg ttatgttcac cgggcgatgt ctctcaggtc tgggtgctgg ttctggtgaa
     3421 tgccggtggt gagccgtttg cggtggttca ggtacagcgg cggtttgcac cggaagccgt
     3481 cagccattca ctggcactgg cggcgtcact ggacgcgcag gggtacagcg tcagtgacat
     3541 catccatatc ctgatggcgg aaggaggtca ggcatgagcg cacgttcaca ggcactggtt
     3601 cctctcagca cagagcaaca ggccgcatgg cgggcggtgg cggagaccga aaaacgccgc
     3661 catcagggta acacgctggc tgaatatccg tatgcggggg cgtttttccg ctgtctgaac
     3721 ggaagccgca ggatatcgct gtctgacctg cgatttttca tgccgtccct gaccgcagaa
     3781 gaactgcacg gcaaccgtct tcagtggctg tatgccatcg atgtgctgat tgaaacacag
     3841 ggcgaggtct gcctccttcc ccttcccggt gatgcagcag agcgcttgtt cccgtcggtg
     3901 cggtttcgtg tcagggaacg cagtcgccat aaatccgcgc tggtcatgca gaagtacagc
     3961 cgccagcagg cgagggaggc agagcagaaa gcccgtgcgt accaggcgct ggtcgctcag
     4021 gctgaaatcg aactggcatt ccattcgccg gaaacagtcg gaagctggca tgcacgctgg
     4081 agtgaccggg tggcagagca tgacctggaa accctgttct ggcagtgggg cgagcgtttt
     4141 ccgtcactgg ccggaatgga acgctggcag tggcaggata tgccattctg gcaggtcatc
     4201 gcagaggctg gtatggcggc aagggtggtc ggtcatgctg ttcgtgagat ggagcggtgg
     4261 atggtgccga acaaactccg ggaggcagcg tgatgcggca acaatcacac ggatttcccg
     4321 tgaacggtct gaatgagcgg attattttca gggaaagtga gtgtggtcag cgtgcaggta
     4381 tatgggctat gatgtgcccg gcgcttgagg ctttctgcct catgacgtga aggtggtttg
     4441 ttgccgtgtt gtgtggcaga aagaagatag ccccgtagta agttaatttt cattaaccac
     4501 cacgaggcat ccctatgtct agtccacatc aggatagcct cttaccgcgc tttgcgcaag
     4561 gagaagaagg ccatgaaact accacgaagt tcccttgtct ggtgtgtgtt gatcgtgtgt
     4621 ctcacactgt tgatattcac ttatctgaca cgaaaatcgc tgtgcgagat tcgttacaga
     4681 gacggacaca gggaggtggc ggctttcatg gcttacgaat ccggtaagta gcaacctgga
     4741 ggcgggcgca ggcccgcctt ttcaggactg atgctggtct gactactgaa gcgcctttat
     4801 aaaggggctg ctggttcgcc ggtagcccct ttctccttgc tgatgttgta cgggcatgaa
     4861 caacctgact tcaggaaggt cgtttccctt caggaacggg gatgaacgcg cgcctgcggc
     4921 gcccgttctt ttcccccgcc ttctctggtt atggcctgtc agaaatcaca tcaccttccc
     4981 tgtgattatt ctctttttct t
//
LOCUS       DAPOZO010000188         4941 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPOZO010000188 DAPOZO010000000
VERSION     DAPOZO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.189"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..815)
                     /locus_tag="ROI21_004654"
     CDS             complement(<1..815)
                     /locus_tag="ROI21_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HDX5113477.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWP"
     gene            1424..2641
                     /locus_tag="ROI21_004655"
     CDS             1424..2641
                     /locus_tag="ROI21_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDX5113478.1"
                     /translation="MATAWYKQVNPPQRKAIFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIFIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWPINGLLPSYLADNGVNTVVISTLMTIAGLGTLTGTIFFGFVGD
                     KIGVKKAFVVGLITSFIFLCPLFFISVKNSSLIGLCLFGLMFTNLGIAGLVPKFIYDY
                     FPTKLRGLGTGLIYNLGATGGMAAPVLATYISGYYGLGVSLFIVTVAFSALLILLVGF
                     DIPGKIYKLSVAK"
     gene            2653..3771
                     /locus_tag="ROI21_004656"
     CDS             2653..3771
                     /locus_tag="ROI21_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDX5113479.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            3814..3939
                     /locus_tag="ROI21_004657"
     CDS             3814..3939
                     /locus_tag="ROI21_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113480.1"
                     /translation="MIKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPHMAS"
     gene            complement(3992..4249)
                     /locus_tag="ROI21_004658"
     CDS             complement(3992..4249)
                     /locus_tag="ROI21_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113481.1"
                     /translation="MVSSSASNVVNCETKQRTQFECIYFSQYWAKGDFIAKRAPIGQW
                     EPYSEESLLGIIVTSVCRIKVAMLKPEPPRDPHIPLMGDFN"
     gene            4239..4475
                     /locus_tag="ROI21_004659"
                     /pseudo
     CDS             4239..4475
                     /locus_tag="ROI21_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4563..4941
                     /locus_tag="ROI21_004660"
                     /pseudo
     CDS             4563..4941
                     /locus_tag="ROI21_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1408 a          940 c         1133 g         1460 t
ORIGIN      
        1 ggccactttt ttcgtgcctg cgggctggtt ttcagcttca ccagatgatc gcctttaccc
       61 agttttctga tctcttcata ttgcgctccc tttctgagag gtatcatcca gtggcggtgt
      121 tctcccgcca ggctccaggc atttaacagt cccagtgagt aataaccttt atccattaac
      181 gtcagagtgt tatcgccggt ttgttctata agttgctcag caagctcatt ttcgctgttc
      241 ttcatcgtgc cgaaggctgc agccgtcagc agatggctgg tcagttccat ctggcagacc
      301 attttgacct gcgggtagag cgccgggttc ccggcatgtg tctggcgggg gaaggctgca
      361 tcgttctctg gtgtatccgg tgtgcgccag aacacaccat cgatggccag cagggtcagg
      421 ccgcaccagt gcggatgcgg cgtggcgtta tgccagagct gcgctgtttt cgtgaacacg
      481 cggcggacag cctcacttcc caggcgctgg cgggcctgaa taacggcact gggggcaacg
      541 aaggggcgat tgcccggcag catgatgtcc aggcgattca caatctggtg aagaggttct
      601 ttacgctcaa gcgccatgcc aacaatacac cagaccatca tttcgagggg aagacggcgc
      661 ttgcgtagcg ttacagtacc tgattcggca aggcaacgag agatgagttc ggggtcgagg
      721 taatccccca gagaagtcag tgggttacgc agagaatcgt aacgggatac cagatcaaga
      781 gcctgtccaa tgtgcataaa aaaatccgga aacaagtgag cgtttccgga ttcttacaca
      841 gccactggat cggtcaactg atccttaact gatcggcatt aatcttggtt ctggtgtttg
      901 taacaaacta tcagctacaa aaatatgctc aatttgtgac atcagtaaca aaacgcgttt
      961 tgttatgtgg attgcttgtt ttttgatctg gtataacagg tataaaggta tacagaaaag
     1021 caaagaaata ctgcaaagga aaacagctat aacgtaagct aaagtaataa cctctcagtc
     1081 tttcctcatt tgacgaaggg agttttattc aacctgaacg gactacgaaa atgagcacaa
     1141 tgaataagtc aatttttgaa gcagggttga actgtgccag tggtggaata tctggcgaac
     1201 attgtttttt gttggtgacc caaaactgta ggtgggtatc agttttatct ttcatagagt
     1261 gaaatatgtt aagaagaaat ggaggaaaag attgtactga ttaggtattg ataacaatca
     1321 atagtactgg cgtatttgaa gacaatataa ttatttctgg atattgttga ggctccctaa
     1381 tatttacttt aagggctata ttagaataac acaggaaaca aatatggcaa cagcatggta
     1441 taaacaagtt aatccaccac aacggaaagc tattttttcc gcatggcttg gatatgtatt
     1501 tgatggcttt gattttatga tgatatttta cattcttcat attataaaag cagatcttgg
     1561 cattacggat attcaggcta ctttaatagg gacagtggcc ttcatagcca gacctattgg
     1621 aggtggtttt tttggtgcca tggctgataa atatggtcgt aagccaatga tgatgtgggc
     1681 aattttcatt tactcagtcg gaacaggcct tagcggtatt gctacaaact tatatatgct
     1741 cgcagtttgc cgttttattg ttggcttagg gatgtctggt gaatatgcat gtgcttcaac
     1801 ttatgcggta gaaagttggc ctaaaaatct tcaatctaaa gctagtgctt ttttggtaag
     1861 tggtttttct gttggaaata ttattgcggc acaaataatc cctcagtttg ctgaagtata
     1921 tggatggaga aactcttttt ttataggcct gttaccagtt ttactagttc tttggatcag
     1981 aaaaagtgct ccagaaagtc aggagtggat tgaagataaa tataaggata aatcaacatt
     2041 tttgtctgtc ttcagaaaac cacatctttc aatctctatg atcgttttcc tcgtctgttt
     2101 ttgtctattt ggtgcaaact ggccgataaa cggactactt ccttcctacc tggcagataa
     2161 tggagttaat acagtggtca tttcaactct gatgacaata gcaggtttag gaacactgac
     2221 aggtacaata ttttttggtt ttgttggtga taagattggt gtaaaaaaag cctttgtagt
     2281 cggtctaata acttcattta ttttcctttg tcctcttttt tttatttctg tgaaaaactc
     2341 ttctcttata ggattatgtc tttttggatt aatgtttaca aatttaggta ttgcagggtt
     2401 ggttccaaaa tttatatatg attactttcc aacaaaatta agaggattag ggaccggtct
     2461 tatttataac ttaggggcaa ctggaggaat ggccgcacct gtattagcta catacatttc
     2521 aggatattat ggcttaggtg tttcattatt cattgttacg gttgcattct ctgccttatt
     2581 aattttgtta gttggttttg atattccagg taaaatttat aaactatccg tggctaaatg
     2641 attggaggct ttatgattaa ttatggcgtt gttggtgttg gatactttgg cgctgaatta
     2701 gctcgtttta tgaatatgca tgataatgca aaaattacat gtgtatacga tcctgaaaat
     2761 ggagaaaata ttgcccgtga attgcagtgt atcaatatgt caagcttgga tgctttagtc
     2821 tcaagtaaat tagtcgattg cgtgatcgta gccaccccaa attatctgca taaagaacca
     2881 gtaattaaag cagcaaagaa taagaagcat gttttttgtg aaaaaccaat tgcattaagt
     2941 tatgaagatt gtgtggatat ggtcaaagcg tgtaaagaag ctggtgtgac ctttatggcc
     3001 gggcatatta tgaatttttt caatggggtt caatatgcac ggaagttaat taaagaaggt
     3061 gttatcggcg aaatattatc atgtcatact aagagaaatg gctgggaaaa caaacaagag
     3121 agactttcct ggaaaaagat gaaagaacaa tctggtggac atctatatca tcatatacat
     3181 gagttagatt gtgttcagca tttacttgga gaaataccag agacggttac tatgattggt
     3241 ggaaatttgg cccattctgg tccaggattt ggcaatgaag atgatatgtt atttatgacc
     3301 ttggaattcc cgtcaggaaa actagcaacc ttagagtggg ggagtgcatt taactggccg
     3361 gaacattatg tcatcatcaa tggaactaaa ggctctatta aaattgatat gcaagaaaca
     3421 gcagggtcac ttaggattgg cggtcagaca aagcattttt tggtccatga aacacaagaa
     3481 gaagatgatg atcgtcggaa aggcaatatg acctcagaaa tggatggcgc tatagcatat
     3541 ggtcatccag gaaaaaaaac accattatgg cttgccagtt taataagaaa ggagacgtta
     3601 ttcctccata atatcctctg tggtgcaaaa cctgaagaag attatattga ccttctcaat
     3661 ggtgaggcgg ccatgtcggc gattgctact gctgatgctg ccactctttc aagatcgcag
     3721 gacaggaaag tgaaaatcag tgagatcatt aaacatacat cagtaatgta aaaactatac
     3781 gcctcttgat ttaattcagg aggcctttta tgtatgatca agaattttat cttcgataat
     3841 ctcataattt tagcagtgcc atttatgata aaaacatccc ttaaaaccaa tctaatcttt
     3901 ttcttcctct gtgtatttgt tccccatatg gcgtcataat ttacgctacg taatacggga
     3961 tagttacgat acgcagcgat agcgctaagt tttagttaaa atcccccatc agcgggatat
     4021 ggggatctct gggaggttca ggttttagca tcgcgacttt aatacgacag acgctggtga
     4081 cgatgatgcc aagtagtgac tcttccgaat aaggttccca ctgaccaatt ggcgcgcgct
     4141 tcgcaataaa atctcccttc gcccagtatt gggaaaagta gatacattca aactgtgtac
     4201 gctgtttcgt ctcacagttc acgacattgc tggccgatga gctgaccatt gggcctatcc
     4261 gggctgtccc gatggatatt acgccgaagt atgtgggaat tgccagcgga ttgatgaacg
     4321 ccggttccgc tgtcgccgac attatttcac ctatcgcctt tggcattatt atcgacaaaa
     4381 caggcaactg gagtttacca ttctacggtt ccgtcgcatt gcttgttatt ggtatattcc
     4441 tgacgttctt catgcgtcca gataagtcat tgtaaaagtc gaatcagggc tgaagtggca
     4501 cactgaattt ggccacctga acagaggtga tatgctcacc tcagaacaac acaggtgctc
     4561 caatgaaaaa aagaaatttc agcgcagagt ttaaacgcga atccgctcaa ctggttgttg
     4621 accagaaata cacggtggca gatgccgcga aagctatgga tgttggcctt tccacaatga
     4681 caagatgggt caaacaactg cgtgatgagc gtcagggcaa aacaccaaaa gcctctccga
     4741 taacaccaga acaaatcgaa atacgtaagc tgaggaaaaa gctacaacgc attgaaatgg
     4801 agaatgaaat attaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg
     4861 ataatcggga aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt
     4921 catcgcagca gctacagata c
//
LOCUS       DAPOZO010000189         4788 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPOZO010000189 DAPOZO010000000
VERSION     DAPOZO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.190"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(158..1000)
                     /gene="purU"
                     /locus_tag="ROI21_004661"
     CDS             complement(158..1000)
                     /gene="purU"
                     /locus_tag="ROI21_004661"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDX5113482.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1050..1508)
                     /locus_tag="ROI21_004662"
     CDS             complement(1050..1508)
                     /locus_tag="ROI21_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDX5113483.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARYTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1621..2526
                     /gene="rssA"
                     /locus_tag="ROI21_004663"
     CDS             1621..2526
                     /gene="rssA"
                     /locus_tag="ROI21_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDX5113484.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            2618..3631
                     /gene="rssB"
                     /locus_tag="ROI21_004664"
     CDS             2618..3631
                     /gene="rssB"
                     /locus_tag="ROI21_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDX5113485.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3833..4741
                     /gene="galU"
                     /locus_tag="ROI21_004665"
     CDS             3833..4741
                     /gene="galU"
                     /locus_tag="ROI21_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDX5113486.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
BASE COUNT         1248 a         1076 c         1214 g         1250 t
ORIGIN      
        1 ggggtaatgc tttttactgg cctgctccct tatcgggaag cggggcgcat catatcaaat
       61 gacgcgccgc tgtaaagtgt tacgttgaga aaaatgaact ggttgcgtaa ttttcacccg
      121 taacggatta aaggtaacca gttatttttg ctggcgatta aagaataatc gtccgattac
      181 cgtaaacaaa gacgcgctgc gccagtactt tgtagagtgc gcgacttaag acgtttttct
      241 cgacgtcacg acctgcgcgc atcatatctt cagctgtgta ggtatgatcg acatgaataa
      301 cgtcctgcat gatgattgga ccttcgtcca gattgtcatt cacatagtga gcggttgcgc
      361 caataatctt cacgccacgt tcataggcct ggtgataagg acgtgcgccg ataaacgctg
      421 gcaggaatga atggtgaata ttgatgatct tattcgggaa gcgtgccaca aattctggcg
      481 ttaatacccg catatacttc gccagcacca cgtagtcagg ctgataagca tcaatggcat
      541 ccgccatctt ttgatcgtgc tcgttgcggc ttaacccttc atggcttacc agctcaaacg
      601 gaatatcaaa acgctcaacc agagaacgta aagtatcgtg gttaccaata actgccgcga
      661 tttcgacatc caggccgccg taattggctt tcatcaacaa atcgccaagg caatgcgcct
      721 ctttagtgac cagaatcact atccggcgac gaccagcagg attcagctca cgtacggagc
      781 cttctggcaa tgcgctgtcg agatccgcca gcagggtgga atcattaaaa attccttcca
      841 gttccgtgcg cataaaaaag cgcccggtac ggtgatcaac aaattcattg ttctgtacga
      901 tatttaactc gtgcttgtag caaatattgg taatacgtgc gatcagacct ttttggtccg
      961 gacaaatagt acgcagaact ttacgttgga gtgaatgcat tgctggaaaa accttgttga
     1021 gagtgtttgc taaaccgtac cgtcaaccat tattggccgc agcacttttt aaatttttta
     1081 cctgaaccac aagggcaggg atcgttgcgg ccaaactgcg gacgtgtacc gtcaatatag
     1141 taccattgac cgttttcttt caaaaatcgc gaacgttcaa tgattgcacc tgttttgcca
     1201 ccttcggtat atcgcgcaac aaaactgaca aatccgatat tgtcgacgtc ctgccaacaa
     1261 tgttcaaata cggtcaatcc taaccattca gtatgcgcaa atccggcaat caattcggca
     1321 cgtaacgccg ccgctccaca agatggatgc caggtcttta ttaaataatc tgcgtctttc
     1381 atcacaaaag cgcagtaacg cgaacgcatg agatgttctg gatcaggtgc aaccttttca
     1441 ccagacacat aagggtggca acataggcta tactcgacag cactaccaca gggacaaagc
     1501 tgagacacaa ataatctccc tggaaacaat aacggcgtat taaccgcctg agtagcacta
     1561 tgttaaccga gcagtagcga tgttgctacg attgcattcc aggggaatct tgcgggaata
     1621 atgagaaaga taaaaatagg gctggcgctg ggatctggcg cggcgagagg ttggtcgcat
     1681 attggcgtta ttaatgcgct aaaaaaagtg ggtattgaaa ttgatatcgt tgcaggatgt
     1741 tcaattggtt cgctggtggg cgctgcctat gcatgcgatc gactatctgc gctggaagat
     1801 tgggtgacct ctttcagtta ttgggatgtt ttacgcctga tggatctctc ctggcagcgc
     1861 ggtgggttac tgcgcggcga gcgtgtcttc aatcaatatc gcgaaataat gccggaaaca
     1921 gagatcgaaa attgttcccg tcgcttcgcg gctgttgcca ccaatttaag tactgggcgc
     1981 gaattatggt ttactgaagg cgatctccat cttgctattc gtgcatcatg cagtattcca
     2041 ggactaatgg cacccgttgc gcataacggc tactggctgg ttgatggcgc tgtagttaac
     2101 ccaattccta tttcgctcac gcgtgcattg ggtgctgata ttgtgatagc ggtcgacctg
     2161 cagcacgatg ctcatttgat gcaacaagat ctgctctcct ttaatgtcag tgaagaaaat
     2221 agcgagaatg gtgattctct gccgtggcat gcgcgtctga aagaaaggtt aggcagcata
     2281 acgacacgtc gggcggtgac agcgccaacg gcaacagaga ttatgaccac ttctatccag
     2341 gtgctggaga accgccttaa aaggaaccgc atggcaggtg atccgcccga tattctgatt
     2401 caacctgttt gcccgcaaat atctacgctt gatttccatc gcgcgcacgc tgccattgcg
     2461 gccgggcagc tggcagtgga aaagaaaatg gacgaacttt tgccgttggt acgcaccaac
     2521 atttgaccag aatttttatc tacacttaag ttaattctga caggcgcagg tggcaatagc
     2581 atgccactat tgagtaaagc cagtcagggg agagaacatg acgcagccat tggtcggaaa
     2641 acagattctc attgttgaag atgagcaggt atttcgctcg cttctggatt cgtggttttc
     2701 ctcattggga gcgacaacgg tactagcggc tgatggggtg gatgcccttg agttgctggg
     2761 aggtttcact ccagacctga tgatatgtga tatcgctatg ccacgaatga acgggcttaa
     2821 actgctggaa catatacgca acagaggtga ccagacacct gttctcgtga tatctgccac
     2881 tgaaaatatg gcagatattg ccaaagcgtt acgtctgggc gttgaagatg ttttgctgaa
     2941 accagttaaa gatctgaatc gtttgcgcga gatggttttt gcctgtctct atcccagcat
     3001 gtttaattcc cgcgttgagg aagaggagag gctttttcgc gactgggatg caatggttga
     3061 taaccctgcc gcagcggcga aattattaca ggaattgcaa ccgccggttc agcaggtgat
     3121 ttcccattgc cgggttaatt atcgtcaatt ggttgccgcg gacaaacccg gcctggtgct
     3181 tgatattgcc gcactttcgg aaaacgatct ggcattttat tgccttgatg tcacccgagc
     3241 tggacataat ggcgtacttg ctgccttgtt attacgcgca ttgtttaacg gattattaca
     3301 ggaacagctt gcacatcaaa atcaacggtt gccagagttg ggcgcgttat tgaagcaggt
     3361 aaaccattta ctccgtcagg ccaatctgcc ggggcagttt ccactattag ttggctatta
     3421 tcatcgcgaa ctgaaaaatc tcattctggt ttctgcgggt ctgaatgcga cgttaaatac
     3481 cggcgaacac caggtgcaaa tcagtaatgg tgttccgtta ggcactttag gtaacgctta
     3541 tttgaatcaa ttgagccagc gatgcgatgc ctggcaatgc caaatttggg gaaccggtgg
     3601 tcggcttcgc ttgatgttgt ctgcagaatg agcaaacgat aacgcgggct aaatttgcat
     3661 taccagctaa tgtcggctgg tggtactatc gtcgccattc gtataagtaa ttgtcttaat
     3721 tatgctaact cgcctccttt tcagaactta gccccttcgg ggtgctgata tactgggatg
     3781 cgatacagaa atatgaacac gttcaaaaca cgaacagtcc aggagaattt aaatggctgc
     3841 cattaatacg aaagtcaaaa aagccgttat ccccgttgcg ggattaggaa ccaggatgtt
     3901 gccggcgacg aaagccatcc cgaaagagat gctgccactt gtcgataagc cattaattca
     3961 atacgtcgtg aatgaatgta ttgcggctgg cattactgaa attgtgctgg ttactcactc
     4021 atctaaaaac tcgattgaaa accactttga taccagtttt gaactggaag caatgctgga
     4081 aaaacgtgta aaacgtcaac tgcttgatga agtgcagtct atttgtccgc cgcacgtgac
     4141 tattatgcaa gttcgtcagg ggctggcgaa aggcctggga catgcggtat tgtgtgctca
     4201 cccggtagtg ggtgatgaac ctgtggctgt tattttgcct gacgttattc tggatgaata
     4261 tgaatccgat ttgtcacagg ataacctggc agagatgatc cgccgctttg atgaaacggg
     4321 tcatagccag atcatggttg aaccggttgc tgatgttacc gcatatggcg ttgtggattg
     4381 caaaggcgtt gaattagcgc cgggtgaaag cgtaccgatg gttggtgtgg tagaaaaacc
     4441 gaaagcggat gttgcgccgt ctaatctcgc tattgtgggt cgttacgtac ttagcgcgga
     4501 tatttggccg ttgctggcaa aaacccctcc gggagctggt gatgaaattc agctcaccga
     4561 cgcaattgat atgctgatcg aaaaagaaac ggtggaagcc tatcatatga aagggaagag
     4621 ccatgactgc ggtaataaat taggttacat gcaggccttc gttgaatacg gtattcgtca
     4681 taacaccctt ggcacggaat ttaaagcctg gcttgaagaa gagatgggca ttaagaagta
     4741 acatccgtat cggtgttatc cacgaaacgg cgttgagcaa tcgacgcc
//
LOCUS       DAPOZO010000190         4450 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPOZO010000190 DAPOZO010000000
VERSION     DAPOZO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.191"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(33..509)
                     /gene="greA"
                     /locus_tag="ROI21_004666"
     CDS             complement(33..509)
                     /gene="greA"
                     /locus_tag="ROI21_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDX5113487.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            757..2190
                     /gene="dacB"
                     /locus_tag="ROI21_004667"
     CDS             757..2190
                     /gene="dacB"
                     /locus_tag="ROI21_004667"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDX5113488.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(2230..3402)
                     /gene="cgtA"
                     /locus_tag="ROI21_004668"
     CDS             complement(2230..3402)
                     /gene="cgtA"
                     /locus_tag="ROI21_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDX5113489.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(3418..4383)
                     /locus_tag="ROI21_004669"
     CDS             complement(3418..4383)
                     /locus_tag="ROI21_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDX5113490.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
BASE COUNT         1067 a         1264 c         1053 g         1066 t
ORIGIN      
        1 aatacatcaa catcttgagt attgggtaat tcttacaggt attccacctt aattacttca
       61 aattctactt cgccgcccgg cgttttgatg accacaacat catcttcttc tttgccgatc
      121 aggccacgag caataggcga gtttacagaa atcaggtttt gtttaaagtc agcttcgtca
      181 tcgccaacga tgcgataagt ctgttcttcg tcagaatcga gattcagcac cgttacggta
      241 gcaccaaaaa taacgcgccc attgttgggc attttggtga catcaatcac ctgcgcgttc
      301 gacagcttgg cttcgatgtc tttgatacgg ccttcgcaga agccctgctg ttcacgagct
      361 gcgtggtatt cggcgttttc tttcaggtcg ccatgctcac gcgcttccgc gatagcagca
      421 atgatttcag gacggcgcac agatttcaga aaatccagct cttcgcgtaa tttttcagcg
      481 ccgcgtaagg tcatcggaat agcttgcatt tgttatacct cttgaatatt cctgatgggg
      541 caagtcttca cccaccccgg ctgttcggcc tgcccggcat aactcctcac cgggaccaga
      601 agcaaaaaaa taccgacccg ggtacaagtc ccaggtcagc tacaattcac attttgatag
      661 tcattttacc ctgaagttcc tgaagggtca tcgtttactt tatagggcgt tgcgccgtag
      721 tatgacggct cgattccagg ttgttagcgc gagattatgc gattttccag atttatcatc
      781 ggattgacca gctgtatagc gttcagtgtt caggccgcaa atgttgatga gtatattact
      841 caactccccg ctggtgccaa ccttgccctg atggtgcaaa aagtcggcgc gtcggccccc
      901 gctattgatt accacagtca gcagatggcg ctgcccgcca gtacgcagaa agtgattaca
      961 gcgctagcgg cgttgattca actcggcccc gattttcgtt ttaccacgac gcttgaaacc
     1021 aaaggtaatg tggaaaacgg cgtacttaag ggtgacttag tggcgcgatt tggtgccgat
     1081 ccgacgttaa aacgtcagga tattcgcaat atggtcgcga ctttgaaaaa atctggcgtc
     1141 aaccaaatcg atggcaatgt gttgatagat acctccattt tcgccagcca cgataaagcc
     1201 cccggctggc catggaatga catgacacaa tgctttagcg ctccgcctgc cgccgccata
     1261 gttgaccgca actgtttctc cgtctcgctc tacagtgccc caaaacctgg tgatatggct
     1321 tttatacgcg tggcatctta ttaccccgtt acgatgttca gccaggtacg caccctcccc
     1381 cgtggttctg ccgaagcgca atactgcgaa ctggatgtcg taccaggcga cctgaaccgc
     1441 tttacgctga cgggatgcct gccgcaacgt tctgagccgc tcccgttggc ttttgccgtg
     1501 caggatggag ccagctatgc cggggcaatt ctgaaagatg agttaaaaca ggcgggtatc
     1561 acctggagcg gaacactgct gcgccagact caggttaacg aacctggaac ggtagttgcc
     1621 agtaaacagt cggccccgct gcacgatctg cttaagatta tgctgaaaaa gtcggacaac
     1681 atgatcgccg atacggtttt ccgcatgata ggccatgcgc gcttcaatgt gcctggaaca
     1741 tggcgggccg ggtcggacgc cgtgcgtcag atcctgcgcc agcaagccgg tgtcgatatt
     1801 ggaaacacca ttattgccga tggttcaggg ctttcgcggc ataacctgat tgcccccgcc
     1861 accatgatgc aggtgctgca atacattgcc caacacgaca atgaacttaa ctttatctcc
     1921 atgctgccgc tggcgggcta tgacggctct ttgcagtacc gtgcaggtct gcatcaggcg
     1981 ggcgtggatg gcaaagtctc agcgaaaacc ggttcgttgc agggggtata taacctggcg
     2041 ggattcatta ccacagcgag cgggcaacga atggcatttg tacaatatct ttctggctat
     2101 gcagtagaac ccgcggatca gcgtaatcgc cgtattccgt tagtgcgttt tgaaagccgt
     2161 ttgtacaaag atatttatca gaacaattag tcaaaaagaa accccggcgc atggccgggg
     2221 ttttgctgat taacgcttgt aaatgaactc aacgccttct tcgtcgtctt cgtcccaatc
     2281 gtcatcccag tcttcatcat cctcttcagc aatctcttca agctgctggc ggtgataatc
     2341 atcccacatg aattcgactt tctctggctg tttcgcttct tcagcctgca cgaccgggtt
     2401 ttcaatgata aaggtcatca catcccagca gagatctttc acgcccagtc cgctcgccgc
     2461 agagatcaga taatatttat cttcccagcc caacgcttca gcgatcgctt tcgctttctc
     2521 ttcggcttct gccttatcca gcagatcgat cttgttgaag actaaccaac gcggtttcgc
     2581 cgccagatcc tggctgtatt tttccagctc gctgataata atacgcgcgt tttcaaccgg
     2641 atcggtgccg tcaatcggat cgatatcgat gaggtgcaac aggacgcggc aacgttccag
     2701 gtgcttcagg aagcgaatgc ccagacctgc gccttccgca gcgccttcaa tcagtcctgg
     2761 aatatcggca acaacgaagc tcttttcgtt gtccattcgt accacaccca gacttggcac
     2821 cagagtggta aacggataat ccgccacttt cggtttagcc gccgataccg cgcgaataaa
     2881 ggtcgattta cccgcgtttg gcatccccaa cataccgacg tcagccagca gcatcagctc
     2941 cagcagcagc tcacgcttat cgcccggcgt accgttagtt ttctgccgtg gtgtacggtt
     3001 aactgacgat ttgaaacggg tattgcccag accgtgccag ccgcccttgg caaccagcag
     3061 acgctgaccg tgtttggtca tatcgcccat ggtttcaccg gtaccctggt cgataacacg
     3121 cgtacctacc ggcactttaa tcgtcacgtc tttaccgcgc ttaccggtac agtcgcggct
     3181 tgcgccattc tgaccacgct ctgcgcggaa agatttttca aaacgataat cgataagcgt
     3241 gttcaggttc tcgtcggctt ccatccatac gtcaccgccg tcaccgccgt cgccgccatc
     3301 cgggccgcct ttcggaatat acttttcgcg gcggaagctc acgcaaccat taccgccatc
     3361 acctgcaacg accagaatcg atgcttcatc aacaaacttc attttattct ccgtaaatca
     3421 ttcgcctgcg cggggttgcg ataccaccgt tgtatgctta cgtaatccgc cccaaatacg
     3481 atgaccaatg gcggaataca tcgcgcccgc aaccacgaca aacgcaccga gataacctaa
     3541 aaggtttaac atcggtctgg cgaagaaatc gggccaggcc agtgataaaa gatctgaaaa
     3601 aaacagcgta aacagtgggg tgagcgtaat gatcgcgctc acctgcgctg cctgccagcg
     3661 agccatcgct tccgccaggg cgccatatcc taccaatgta ttcagtccgc aaaaaattaa
     3721 acatgcgagc tgccagtggc taagctgcgc tatcactcca ggctttgcca gagggaagag
     3781 cgcaattgta cataaagtgt acagtaaaaa cagaatctgc ggtgaggcca gccgacgcaa
     3841 taaaaccttt tgcgccacgc cataactcac ccaaaccgtc gcagcaccga ccccaaagat
     3901 aactccccag gtgtaatcgg tgagctttgt aaatatctcg accagactgg tgttaaaaaa
     3961 catcaccagg ccgctcagga gcatcaatgc ccctacaacc tgagtgctgc gcattttctc
     4021 tttcaggata aatacgctgg caaccatcat gccaactggc gagagttgcc caatcacctg
     4081 cgaagcggtc ggactcaggt attgcaagga tgagctgaac aggatgaagt tcccaaacag
     4141 cccggcggtc gccactgcca acaaaatcaa ccagcgtggc ttacgaaaca cgcgtaatgg
     4201 cggcaacctc ttcttcaccg caagaatggc accaaggcca atactcgcca tcaagaaacg
     4261 gtaaaacacg attgtcggag gttccatcac ctccagcacc tgcttcattg cgattggcaa
     4321 cgccccccag caaattgctg tggtgagcgc caaaagaatg ccaatgcctg cctgctgctt
     4381 cataccgtat tccctgcaaa gcgcatttgc gaattaacgt cgcaccatgc gcatttttac
     4441 cggttatcga
//
LOCUS       DAPOZO010000191         4347 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPOZO010000191 DAPOZO010000000
VERSION     DAPOZO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.192"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            211..1068
                     /gene="focA"
                     /locus_tag="ROI21_004670"
     CDS             211..1068
                     /gene="focA"
                     /locus_tag="ROI21_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDX5113491.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1123..3405
                     /gene="pflB"
                     /locus_tag="ROI21_004671"
     CDS             1123..3405
                     /gene="pflB"
                     /locus_tag="ROI21_004671"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDX5113492.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3597..4337
                     /gene="pflA"
                     /locus_tag="ROI21_004672"
     CDS             3597..4337
                     /gene="pflA"
                     /locus_tag="ROI21_004672"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDX5113493.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         1111 a         1072 c         1066 g         1098 t
ORIGIN      
        1 tgttttagcg gaggatgcgg aaaaaattca actcatttgt taatttttaa aatttatttt
       61 tatttggata atcaaatatt tactccgtat ttgcataaaa accatgcgag ttacgggcct
      121 ataagccagg cgagatatga tctatatcaa tttctcatct ataatgcttt gttagtatct
      181 cgtcgccgac ttaataaaga gagagttagt gtgaaagctg acaacccttt tgatctttta
      241 cttcctgcag caatggccaa agtggccgaa gaggcaggtg tctataaagc aacgaaacat
      301 ccgcttaaga ctttctatct ggcgattacc gccggtgttt tcatctcaat cgcattcgtc
      361 ttctatatca cagcaaccac tggcacagga acaatgccct tcggcatggc aaaactggtt
      421 ggcggcattt gcttctctct ggggctgatt ctttgtgttg tctgcggagc cgatctcttt
      481 acttccacag tgttgattgt tgttgctaag gcgagtgggc gaatcacctg gggtcagttg
      541 gcgaaaaact ggctaaatgt ctattttggc aacctggtcg gcgcactgct gtttgtactt
      601 ttaatgtggc tttccggcga gtatatgacc gcgaatggtc aatggggact aaacgtccta
      661 caaaccgccg accacaaagt gcaccatact tttattgagg ccgtctgcct tggtatcctg
      721 gcaaacctga tggtatgtct ggcagtatgg atgagttatt ctggccgcag cctgatggat
      781 aaagcgttca ttatggtgct gccggtcgcg atgtttgttg ccagcggttt tgagcacagt
      841 atcgcaaaca tgtttatgat cccgatgggt attgtaatcc gcgacttcgc atctccggaa
      901 ttctggaccg ctgtcggttc tgcaccggaa aatttttctc acctgaccgt gatgaacttc
      961 atcactgata acctgattcc ggttacgatc ggtaatatta tcggcggtgg tttgttggtt
     1021 gggttgacat actgggtcat ttacctgcgt gaaaacgatc accattaatg gttgtcgaag
     1081 tacgcagtaa ataaaaaatc cacttaagaa ggtaggtgtt acatgtccga gcttaatgaa
     1141 aagttagcca cagcctggga aggttttacc aaaggtgact ggcagaatga agtaaacgtc
     1201 cgtgacttca ttcagaaaaa ctacactccg tacgagggtg acgagtcctt cctggctggc
     1261 gctactgaag cgaccaccac cctgtgggac aaagtaatgg aaggcgttaa actggaaaac
     1321 cgcactcacg cgccagttga ctttgacacc gctgttgctt ccaccatcac ctctcacgac
     1381 gctggctaca tcaacaaagc gttggaaaaa gttgttggtc tgcagactga agctccgctg
     1441 aaacgtgctc ttatcccgtt cggtggtatc aaaatgatcg aaggttcctg caaagcgtac
     1501 aaccgcgaac tggacccgat gatcaaaaaa atcttcactg aataccgtaa aactcacaac
     1561 cagggcgtgt tcgacgttta cactccggac atcctgcgtt gccgtaaatc cggtgttctg
     1621 accggtctgc cagatgctta tggccgtggt cgtatcatcg gtgactaccg tcgcgttgcg
     1681 ctgtacggta tcgactacct gatgaaagat aaacttgcac agttcacctc tctgcaggct
     1741 gatctggaaa acggcgtaaa cctggaacag actatccgtc tgcgcgaaga aatcgctgaa
     1801 cagcaccgcg ctctgggtca gatgaaagaa atggctgcga aatacggcta cgacatctct
     1861 ggtccggcta ccaacgctca ggaagctatc cagtggactt acttcggcta cctggctgct
     1921 gttaagtctc agaacggtgc tgcaatgtcc ttcggtcgta cctccacctt cctggatgtg
     1981 tacatcgaac gtgacctgaa agctggcaag atcaccgaac aagaagcgca ggaaatggtt
     2041 gaccacctgg tcatgaaact gcgtatggtt cgcttcctgc gtactccgga atacgatgaa
     2101 ctgttctctg gcgacccgat ctgggcaacc gaatctatcg gtggtatggg cctcgacggt
     2161 cgtaccctgg ttaccaaaaa cagcttccgt ttcctgaaca ccctgtacac catgggtccg
     2221 tctccggaac cgaacatgac cattctgtgg tccgaaaaac tgccgctgaa cttcaagaaa
     2281 ttcgctgcta aagtgtccat cgacacctct tctctgcagt atgagaacga tgacctgatg
     2341 cgtccggact tcaacaacga tgactacgct atcgcttgct gcgtaagccc gatgatcgtt
     2401 ggtaaacaaa tgcagttctt cggtgcgcgt gcaaacctgg cgaaaaccat gctgtacgca
     2461 atcaacggtg gcgttgacga aaaactgaaa atgcaggttg gtccgaagtc tgagccgatc
     2521 aaaggcgatc tcctgaacta cgatgaagtg atggagcgca tggatcactt catggactgg
     2581 ctggctaaac agtacatcac tgcactgaac atcatccact acatgcacga caagtacagc
     2641 tacgaagcct ctctgatggc gctgcacgac cgtgacgtta tccgcaccat ggcgtgtggt
     2701 atcgctggtc tgtccgttgc tgctgactcc ctgtctgcaa tcaaatatgc gaaagttaaa
     2761 ccgattcgtg acgaagacgg tctggctatc gacttcgaaa tcgaaggcga atacccgcag
     2821 tttggtaaca acgatccgcg tgtagatgac ctggctgttg acctggtaga acgtttcatg
     2881 aagaaaattc agaaactgca cacctaccgt gacgctatcc cgactcagtc tgttctgacc
     2941 atcacttcta acgttgtgta tggtaagaaa actggtaaca ccccagacgg tcgtcgtgct
     3001 ggcgcgccgt tcggaccggg tgctaacccg atgcacggtc gtgaccagaa aggtgcagta
     3061 gcctctctga cttccgttgc taaactgccg tttgcttacg ctaaagatgg tatctcctac
     3121 accttctcta tcgttccgaa cgcactgggt aaagacgacg aagttcgtaa gaccaacctg
     3181 gctggtctga tggatggtta cttccaccac gaagcatcca tcgaaggtgg tcagcacctg
     3241 aacgttaacg tgatgaaccg tgaaatgctg ctcgacgcga tggaaaaccc ggaaaaatat
     3301 ccgcagctga ccatccgtgt atctggctac gcagtacgtt tcaactcgct gactaaagaa
     3361 cagcagcagg acgttattac tcgtaccttc actcaatcta tgtaattaga tttgactgaa
     3421 atcgtacagt aaaaagcgta caataaaggc tccacgaaag tggggccttt tttagcacga
     3481 gagccttttt tgtcagctat ctatacttta aggtgactgc caaaacagac tcgacgtagc
     3541 cttcgagctg cgcaccaaca cggcctcaga tgggccacat ctggagaaac accgcaatgt
     3601 cagttattgg tcgcattcac tcctttgaat cctgtggaac cgtagacggc ccgggtattc
     3661 gctttatcac ctttttccag ggctgcctga tgcgctgcct gtattgtcat aaccgcgaca
     3721 cctgggacac gcatggcggt aaagaagtta ccgttgaaga tttaatgaaa gaggtggtga
     3781 cctatcgcca ctttatgaac gcttccggcg gcggcgttac cgcatccggc ggtgaagcaa
     3841 tcctgcaagc tgagtttgtt cgtgactggt tccgcgcctg caaaaaagaa ggcattcata
     3901 cctgtctgga caccaacggt tttgttcgtc gttacgatcc ggtgattgat gaactgctgg
     3961 aagtaaccga cctggtaatg ctcgatctca aacagatgaa cgacgagatc caccaaaatc
     4021 tggttggagt ttcaaaccac cgcacgctgg agttcgccaa atatctggcg aacaaaaatg
     4081 tgaaggtgtg gatccgctac gttgttgtcc caggctggtc tgacgatgac gattcagcgc
     4141 atcgcctcgg tgaatttacc cgtgatatgg gcaacgttga gaaaatcgag cttctcccct
     4201 accacgaact tggcaaacac aaatgggtgg cgatgggtga agaatacaaa cttgatggtg
     4261 ttaaaccacc gaagaaagag accatggaac gcgtgaaagg cattcttgag cagtacggtc
     4321 ataaggtaat gttctaattt ccccgta
//
LOCUS       DAPOZO010000192         4274 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPOZO010000192 DAPOZO010000000
VERSION     DAPOZO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.193"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..157)
                     /locus_tag="ROI21_004673"
                     /pseudo
     CDS             complement(1..157)
                     /locus_tag="ROI21_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(169..357)
                     /locus_tag="ROI21_004674"
     CDS             complement(169..357)
                     /locus_tag="ROI21_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDX5113494.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            629..2200
                     /gene="bcsE"
                     /locus_tag="ROI21_004675"
     CDS             629..2200
                     /gene="bcsE"
                     /locus_tag="ROI21_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDX5113495.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEGITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVGLRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQNSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            2197..2388
                     /gene="bcsF"
                     /locus_tag="ROI21_004676"
     CDS             2197..2388
                     /gene="bcsF"
                     /locus_tag="ROI21_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDX5113496.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            2385..4064
                     /gene="bcsG"
                     /locus_tag="ROI21_004677"
     CDS             2385..4064
                     /gene="bcsG"
                     /locus_tag="ROI21_004677"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDX5113497.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(4179..>4274)
                     /locus_tag="ROI21_004678"
     CDS             complement(4179..>4274)
                     /locus_tag="ROI21_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDX5113498.1"
                     /translation="IQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT         1040 a         1089 c         1033 g         1112 t
ORIGIN      
        1 aaaatcaacg ttaaatgaca ggcgcaacaa gttgtccggg caggcatcga ccaccaggac
       61 attttctccc agcatttgta atgaccaggc taatgcggcg gtgatggttg ttgtccccac
      121 gcctcccccg caccccctgc aatcccagta cggccattca ccaatcccct acttttgttg
      181 cgcaaactct gccagcaacg gccagcgttt taatgccgcg gccaactgtt cgcgttggga
      241 aatatcggca taatcaatat caggcagtga aaatgcctgc tttaacgcca aaatatcatt
      301 ctggaagata tagcctatcg cgggatcagg cagagtatct ggttcattgt tattcatttg
      361 cttgatccct atgaaataac cgtaccgttc atgagacaat tctaaccgtg agcatttcgc
      421 cggtttattg tttacggcga gacgggaatt tgctcacttt ttgactttcc gttacgcaac
      481 aaaaaaagtt aaaaacgaaa agactaaaat cacacgtctt gtgacctgct aaattgattc
      541 acatgctaaa tctgataagt tttaatttca atggtaggtt tatttcttag ctttcgctag
      601 taaactgata atcagacaaa atgatgacat gagggacatt gtggaccctg tattctctat
      661 cggtatctca tcattatggg atgagctgcg acatatgcca gcaggcggcg tctggtggtt
      721 taacgtcgat cgccatgaag atgctatcag tctggcgaat caaacaattg catcccaggc
      781 tgaaaccgca cacgtcgcgg tcattagcat ggacagcgat ccggcgaaaa tctttcaatt
      841 agatgattct caaggaccgg aaaaaataaa attattttca atgctaaatc atgaaaaagg
      901 tctatactat ttggcccgtg atttgcagtg ttctattgat ccccataatt acctttttat
      961 tcttgtttgc gcaaataacg catggcaaaa cattcctgcc gagcggcttc gctcatggtt
     1021 ggataaaatg aataaatgga gcaggttaaa ccattgttcg cttttggtaa ttaatcccgg
     1081 aaataataac gataaacaat tttcattgtt gcttgaggaa taccgttcac tttttggtct
     1141 tgccagtttg cgttttcagg gcgaccaaca tttgctggat attgccttct ggtgcaacga
     1201 aaaaggggtt agcgcccgtc agcagcttag cgttcagcaa caaaatggta tctggacatt
     1261 agttcaaagc gaagaggcgg agatccaacc acgcagcgac gaaaaacgca ttctgagtaa
     1321 tgttgctgta ctggaaggtg cgccgccgct atcagaacac tggcaactgt tcaacaataa
     1381 cgaagtcctg ttcaatgaag cccgtaccgc tcaggcggcg acggtggtct tttctttaca
     1441 acaaaatgcg caaatcgagc cactggcccg cagcattcat actctgcgtc gccagcgcgg
     1501 tagtgcgatg aaaatcctcg tacgggaaaa taccgctagc ctgcgcgcca ccgatgaacg
     1561 tttgttattg gcctgcggtg caaatatggt tatcccatgg aatgccccac tctcccgctg
     1621 tctgacgatg atcgaaagcg tgcaagggca gaagtttagt cgctatgtgc cggaaggtat
     1681 cactaccttg ctgtcaatga cccagccgct caaactgcgt ggtttccaga agtgggatgt
     1741 gttctgtaat gccgtcaaca acatgatgaa taaccctcta ttacctgccc acggtaaagg
     1801 cgttctggtt ggcctacgtc cggtaccggg tatccgcgtt gagcaagccc tgacgctgtg
     1861 tcgccctaac cgcaccggcg atatcatgac tattggcggt aatcggctgg tgctgtttct
     1921 ctcattctgt cggattaacg atctggatac cgcgttgaat catattttcc cattgccgac
     1981 tggcgacatt ttctcaaacc gtatggtctg gtttgaagat gatcaaatca gtgccgagct
     2041 ggtgcagatg cgcctgcttg ccccagaaca atggggcatg ccgctgcctt taacgcaaaa
     2101 ttctaaaccg gtcattaatg ccgagcacga tggtcgccac tggcgacgaa taccagaacc
     2161 aatgcgactg ttagatgatg ctgtggagcg ctcatcatga tgaccatcag cgatatcatt
     2221 gaaattattg tcgtttgcgc actgatattt ttcccgctgg gctatctggc gcggcactct
     2281 ttgcgacgca ttcgcgacac cttacgtttg ttctttgcta aacctcgtta tgttaaaccg
     2341 gccgggacgt tacgccgcac ggaaaaagcc agggcaacca aaaaatgact caatttacgc
     2401 aaaataccgc catgccttct tccctctggc aatactggcg cggcctttcc ggctggaact
     2461 tctattttct ggttaagttc ggcctgttgt gggcgggata tcttaacttc catccgctcc
     2521 tcaatctggt gtttgccgcg tttctgctga tgcccattcc gcgctacagc ctgcatcgct
     2581 tacgccactg gattgccctg ccgatcggct ttgctttgtt ctggcatgac acctggttgc
     2641 ctggcccgga aagcataatg agccagggtt cgcaggtggc ggggttcagt accgattatt
     2701 taatcgacct tgtcacacgc tttattaact ggcagatgat tggggccatt tttgttttat
     2761 tagtggcctg gttattcctg tcacaatgga ttcgcattac cgtttttgtg gttgccattc
     2821 tgctatggct gaacgtactt accctggcgg gaccaagttt ctccttgtgg ccagccggtc
     2881 aaccgacgac cactgtaaca acgacgggtg gcaacgcagc ggcaaccgtt gcggcgacgg
     2941 gtggcgcacc ggtagtgggt gatatgcccg cacaaactgc accgccaaca acggcgaacc
     3001 ttaacgcctg gctgaataat ttctataacg cggaggcgaa acgtaaatcg accttcccgt
     3061 cttcgctgcc cgctgatgct caaccatttg aactactggt gattaatatc tgttcgcttt
     3121 cctggtcgga tattgaagcc gccgggttga tgtcgcatcc gctgtggtcg catttcgata
     3181 ttgagttcaa gaactttaac tccgccacct cctacagtgg cccggcggcg atccgtttac
     3241 tgcgcgccag ctgcgggcag acttcgcaca ctaatctgta tcaaccggca aataacgact
     3301 gctatctgtt tgataacctg tcgaaactgg gctttaccca gcacctgatg atgggacata
     3361 acggccagtt cggcggtttt ttgaaagaag ttcgcgaaaa tggcggcatg cagaccgaat
     3421 tgatggatca aacaaatctg ccggttattt tgctgggctt tgatggttcg ccggtttatg
     3481 acgataccgc cgtgcttaac cgctggctgg acgttaccga aaaagataaa aacagccgta
     3541 gtgccacgtt ctacaacacg cttccactgc atgacggcaa ccattatccg ggcgtcagca
     3601 aaacagcgga ttacaaagcg cgggcgcaga aattctttga tgaactggac gccttcttta
     3661 ctgaactgga gaaatcgggt cgtaaagtga tggtggtcgt ggtgccggaa cacggcggcg
     3721 cgctgaaggg cgacagaatg caggtatctg gcctacgtga tatccctagc ccgtctatca
     3781 cagacgtccc cgttggggtg aaattcttcg gcatgaaggc accacatcag ggggcaccga
     3841 ttgtcatcga ccaaccgagc agcttcctgg ctatctccga tctggtggtt cgcgttcttg
     3901 atggcaagat tttcaccgaa gacaatgttg actggaaaaa actcaccagt gggttgccac
     3961 aaacagcacc ggtctcagag aactcaaatg cagtagttat tcaataccag gataaaccgt
     4021 acgttcgcct gaacggcggc gactgggtgc cttacccgca gtaagcacga ataaatatcc
     4081 tccggcatag ccggaggttt ttcaaatgcg cctataaggc tctcttacca gccgcgccct
     4141 aacaggcgca cacgatctga catttgcatc caacttcgtt acttacggcc cgtaaacggg
     4201 ctgcccggat agggaatcga taactgctct cccattttat cctcttcaag ctggtgcttt
     4261 atgtaatcct gtat
//
LOCUS       DAPOZO010000193         4217 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPOZO010000193 DAPOZO010000000
VERSION     DAPOZO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.194"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="ROI21_004679"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="ROI21_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDX5113499.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ROI21_004680"
     tRNA            complement(324..399)
                     /locus_tag="ROI21_004680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ROI21_004681"
     tRNA            complement(406..480)
                     /locus_tag="ROI21_004681"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ROI21_004682"
     tRNA            complement(597..681)
                     /locus_tag="ROI21_004682"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ROI21_004683"
     tRNA            complement(690..765)
                     /locus_tag="ROI21_004683"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ROI21_004684"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ROI21_004684"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDX5113500.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAINTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2109..3071)
                     /gene="birA"
                     /locus_tag="ROI21_004685"
     CDS             complement(2109..3071)
                     /gene="birA"
                     /locus_tag="ROI21_004685"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDX5113501.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHMQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRTALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAE"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ROI21_004686"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ROI21_004686"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDX5113502.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQSVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPITVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VGAVETIS"
BASE COUNT         1088 a         1044 c          951 g         1134 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca gtgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 gactgatggc ttccttcacc ctaatcaggt actgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cacctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta atactgccat gacgttgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcaggctacg caaataattt tcaggggagc taatgctccc
     2101 ctatcttatt attccgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agcgccctgt ttgtctattc cgcgcgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgc tcgaagagtt ccagcgccgt gcgtaattca
     2341 cgtatgagca tggccgccaa cgtattacga tcgagattga tcccggcttc ctgcagcgtg
     2401 atccaccctt gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggca
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtta gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttaa cacgaacttt atcagcaccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataacgggc
     2821 agcacagtta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gcatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aatagggcga tcaatttcag tggcacggtg
     3061 ttatccttca tgaaatcgtc tccactgcgc ccacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcagcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttgggtca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt ataggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccaattca acacagtcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtaccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac ggattgtcct tttgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagtgtttg tagaaacgca aaaaggccca
     4201 gtcttccgac tgggcct
//
LOCUS       DAPOZO010000194         4173 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPOZO010000194 DAPOZO010000000
VERSION     DAPOZO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.195"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            47..1348
                     /gene="dcuA"
                     /locus_tag="ROI21_004687"
     CDS             47..1348
                     /gene="dcuA"
                     /locus_tag="ROI21_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDX5113503.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            1463..1801
                     /gene="cutA"
                     /locus_tag="ROI21_004688"
     CDS             1463..1801
                     /gene="cutA"
                     /locus_tag="ROI21_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDX5113504.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            1777..3474
                     /gene="dsbD"
                     /locus_tag="ROI21_004689"
     CDS             1777..3474
                     /gene="dsbD"
                     /locus_tag="ROI21_004689"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDX5113505.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTITYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            3511..4086
                     /locus_tag="ROI21_004690"
     CDS             3511..4086
                     /locus_tag="ROI21_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDX5113506.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT          924 a         1117 c         1076 g         1056 t
ORIGIN      
        1 aaataacaat ctaatatcaa cttgttaaaa aacaaggaag gctaatatgc tagttgtaga
       61 actcatcata gttttgctgg cgatcttctt gggcgccaga ttggggggaa taggtattgg
      121 ttttgcaggc ggattggggg tgctggttct tgccgctatt ggcgttaaac ccggtaacat
      181 cccgttcgat gtcatttcca ttatcatggc ggttatcgcc gctatttctg ccatgcaggt
      241 tgctggcggt ctggactatc tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa
      301 atacatcacg atcctcgcac cgatcgtgac ctatttcctg actatctttg ctggtactgg
      361 caacatctct ctggcgacac tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa
      421 accttgccgt ccgctgtcta ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc
      481 aatctcagcg gcagtggttt acatgtcttc cgtaatggaa ggtcatggca tcagctacct
      541 ccatctgctc tccgtggtca tcccgtccac cctgctggcg gttctggtga tgtccttcct
      601 ggtcactatg ctgttcaact ccaaactctc tgacgatccg atttatcgca agcgtctgga
      661 agaaggcctg gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac
      721 gtccgtctgg ctgttcctgc tgggcgtagt tggcgttgtt atctatgcaa tcatcaacag
      781 cccaagcatg ggtctggttg aaaaaccgct gatgaacacc accaacgcaa tcctgatcat
      841 catgctcagc gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg acaacatcct
      901 caactccagc accttcaaag caggtatgag cgcctgtatt tgtatcctgg gtgttgcatg
      961 gctgggcgat actttcgttt ccaacaacat cgactggatc aaagataccg ctggtgaagt
     1021 gattcagggt catccgtggc tgctggccgt catcttcttc tttgcttctg ctctgctgta
     1081 ctctcaggct gcaaccgcaa aagcactgat gccgatggct ctggcactga acgtttctcc
     1141 gctgaccgct gttgcttctt tcgctgcggt gtctggtctg ttcattctgc cgacctaccc
     1201 gacgctggtt gctgcggtac agatggatga cacgggtact acccgtatcg gtaaattcgt
     1261 cttcaaccat ccgttcttca tcccgggtac tctgggtgtt gcccttgccg tttgcttcgg
     1321 cttcgtgctg ggtagcttca tgctgtaatg accaatcgcg gggcttcagg ccccgctttc
     1381 tttcccgccg actaacatcc ttcccccgtc cgttgtatag tgacctctct cttgcggttc
     1441 catctgttct tgcgaggtgt ttatgcttga tgaaaaaagt tcgaataccg cgtctgtcgt
     1501 ggtgctatgt acggcaccgg atgaagcgac agcccaggat ttagccgcca aagtgctggc
     1561 ggaaaaactg gcggcctgcg cgaccttgat ccccggcgct acctctctct attactggga
     1621 aggtaagctg gagcaagaat acgaagtgca gatgatttta aaaactaccg tatctcacca
     1681 gcaggcactg ctggaatgcc tgaagtctca tcatccatat caaaccccgg aacttctggt
     1741 tttacctgtt acacacggag acacagatta cctctcatgg ctcaacgcat ctttacgctg
     1801 atcctgctac tttgcagcac ttccgttttt gccggattat tcgacgcgcc gggacgttca
     1861 caatttgtcc ccgcggatca agcctttgct tttgattttc agcaaaacca acatgacctg
     1921 aatctgacct ggcagatcaa agacggttac tacctctacc gtaaacagat ccgcattacg
     1981 ccggaacacg cgaaaattgc cgacgtgcag ctgccgcaag gcgtctggca tgaagatgag
     2041 ttttacggca aaagcgagat ttaccgcgat cggctgacgc ttcccgtcac catcaaccag
     2101 gcgagtgcgg gagcgacgtt aactatcacc taccagggct gtgctgatgc cggtttctgt
     2161 tatccgccag aaaccaaaac cgttccgtta agcgaagtgg tcgccaacaa cgcagcgtca
     2221 cagcctgtgt ctgttccgca gcaagagcag cccaccgcgc aattgccctt ttccgcgctc
     2281 tgggcgttgt tgatcggtat tggtatcgcc tttacgccat gcgtgctgcc aatgtaccca
     2341 ctgatttctg gtatcgtgct gggcggtaaa cagcggctct ccactgccag agcattgttg
     2401 ctgaccttta tttatgtgca ggggatggcg ctgacttaca cggcactggg tctggtggtt
     2461 gccgccgcag ggttacagtt ccaggcggcg ctacagcacc catacgtgct cattggcctc
     2521 gctatcgtct ttaccttgct ggcgatgtca atgtttggct tgtttactct gcaactcccc
     2581 tcttcgctgc aaacacgtct cacgctgatg agcaatcgcc aacagggcgg ctcacctggc
     2641 ggtgtgtttg ttatgggggc gattgccgga ctgatctgtt caccatgcac caccgcaccg
     2701 cttagcgcga ttctgctgta tatcgcccaa agcgggaaca tgtggctggg gggcggcacg
     2761 ctttatctct atgcgttggg catgggcctg ccgctgatgc taattaccgt ctttggtaac
     2821 cgcttgctgc cgaaaagcgg cccgtggatg gaacaagtca aaaccgcgtt tggttttgtg
     2881 atcctcgcac tgccggtctt cctgctggag cgagtgattg gtgatatatg gggattacgc
     2941 ttgtggtcgg cgcttggtgt cgcattcttt ggctgggcct ttatcaccag cctacaggcc
     3001 aaacgcggct ggatgcgcgt ggtgcaaata atcctgctgg cagcggcatt ggttagcgtg
     3061 cgcccacttc aggattgggc atttggtgcg acgcataccg cgcaaactca gacgcatctc
     3121 aactttacac aaatcaaaac ggtagatgag ttaaatcagg cgctcgttga agccaaaggc
     3181 aaaccagtga tgttagatct ctatgccgac tggtgcgtcg cctgtaaaga gtttgagaaa
     3241 tacaccttca gcgacccgca ggtgcaaaaa gcgttagcag acacggtatt acttcaggcc
     3301 aacgtcactg ccaacgacgc acaagatgtg gcgctgttaa agcatcttaa tgtccttggc
     3361 ctaccgacaa ttctcttttt tgacggacaa ggccaggagc atccacaagc acgcgtcacg
     3421 ggctttatgg atgctgaaac cttcagcgca catttgcgcg atcgccaacc gtgaacgaca
     3481 cttgcagtgg gaaagacgga ggaaaatagc gtgcaacgtg aagatgtact gggagaagcc
     3541 ctgaaattat tagaattaca agggattgcc aacaccacgc tggagatggt tgctgaacgt
     3601 gtggattatc cactggacga gctacgccgc ttctggccag acaaagaggc aatcctctac
     3661 gatgcgctgc gttatcttag ccaacagata gatgtctggc gtcgtcagct gatgctggac
     3721 gaaacgcaaa ccgccgaaca aaagttgctg gcacgttatc aggcgttatc ggagtgcgtt
     3781 aaaaacaacc gctatccggg ctgtctgttt atcgctgcct gtacgtttta tcccgatcct
     3841 ggccacccta ttcatcaact ggccgatcag caaaaaagcg cggcctacga tttcacccac
     3901 gaactgttaa ccacactgga agttgacgat ccggcgatgg tagcaaagca gatggaactg
     3961 gtgctggaag gctgtttaag ccgaatgctg gtgaatcgta gccaggcgga tgtcgacacc
     4021 gcacatcggc tggcggaaga tatcctgcgc ttcgcccgct gccgccaggg tggtgctttg
     4081 acctgacaga aacacagaaa agaagcgatt tgccgcaatc ttaagcagtt gaatcgcttt
     4141 tactgaaatt aggttgacga gatgtgcaga tta
//
LOCUS       DAPOZO010000195         4154 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPOZO010000195 DAPOZO010000000
VERSION     DAPOZO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.196"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..83
                     /gene="hokD"
                     /locus_tag="ROI21_004691"
     CDS             <1..83
                     /gene="hokD"
                     /locus_tag="ROI21_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDX5113507.1"
                     /translation="RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            300..551
                     /locus_tag="ROI21_004692"
     CDS             300..551
                     /locus_tag="ROI21_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113508.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            618..896
                     /locus_tag="ROI21_004693"
     CDS             618..896
                     /locus_tag="ROI21_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113509.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            898..1947
                     /locus_tag="ROI21_004694"
     CDS             898..1947
                     /locus_tag="ROI21_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDX5113510.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALTIGVLA"
     gene            1961..2713
                     /locus_tag="ROI21_004695"
     CDS             1961..2713
                     /locus_tag="ROI21_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDX5113511.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEASTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(2991..3080)
                     /locus_tag="ROI21_004696"
     CDS             complement(2991..3080)
                     /locus_tag="ROI21_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113512.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(3135..3347)
                     /gene="cspF"
                     /locus_tag="ROI21_004697"
     CDS             complement(3135..3347)
                     /gene="cspF"
                     /locus_tag="ROI21_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HDX5113513.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            3648..3863
                     /gene="cspB"
                     /locus_tag="ROI21_004698"
     CDS             3648..3863
                     /gene="cspB"
                     /locus_tag="ROI21_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HDX5113514.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT         1141 a          877 c         1052 g         1084 t
ORIGIN      
        1 cgaggaaaga cctctgcgag gtacgaatcc gaaccggcca gacggaggtc gctgtcttca
       61 cagcttacga acctgaggag taagagaccc ggcgggggag aaatccctcg ccacctctga
      121 tgtggcaggc atcctcaacg cacccgcact taacccgctt cggcgggttt ttgtttttat
      181 tttcaacgcg tttgaagttc tggacggtgc cggaatagaa tcaaaaatac ttaagtagcg
      241 cgcagggata agagggatgg tcccttaaag gggagagcta attatccgga aggattctga
      301 tgatgaacat cgaagaactg cgtaaaattt tttgtgaaga tggcctctat gctgtgtgcg
      361 ttgaaaatgg aaatcttgtt agtcattacc gcattatgtg tttgcgaaag aatggggctg
      421 cgttaattaa ttttgtggat gctcgggtca cggacggatt tatcttgcgc gaaggtgagt
      481 ttgtcacttc attacaggca ttgaaagaga tcggaataaa agctggcttt tctgcttttt
      541 caggagaata aactcatcta caatcttgcg cggggctgaa ctcccgctga gtaacaccgt
      601 gccaccggag aaaaccgatg gcacgcaacg caaaatatta caattctgat aattcgcccg
      661 ttcttgcctg cacgcacggg cggtattctc acgcattcaa gtctgaatgg ttccagcacc
      721 ctccatgcac tgcagaacag gccgaatggc tgattcattc ttaccgcagg cgcgggttcg
      781 aggttaagaa agctctcagt ctcgactatc ggcactggat aatctctgtc aggctgcctt
      841 attccgaacg cccaccacgt gcgtcccgca ctttccagca acggatctgg aggtaacgtg
      901 cgggtattac ttagacctgt tctggtgcct gagcttgggc tggtggtcct taagccgggc
      961 cgtgaatcca tacagatatt tcataatcct cgagtgctgg tggaaccgga accaaaaagc
     1021 atgcgtaatc tgccatccgg agtcgttcct gccgttcgcc agccgctggc ggaagacaaa
     1081 acattgctgc cgttttttag taacgaacgg gtgattcgtg ctgctggcgg cgttggcgca
     1141 ttgtccgact ggctattacg tcatgttaca tcctgccagt ggcctaatgg cgattaccat
     1201 cacactgaaa cagtcattca ccgttatggt accggcgcaa tggtgttgtg ctggcactgc
     1261 gacaaccaac tgcgtgacca gacatcggaa tcactggagc tgcttgctca acaaaatctg
     1321 acagcatggg tgattgacgt catccgtcac gcaataagcg gtacgcagga gcgggaatta
     1381 tctttggctg aattatcctg gtgggcggtc tgcaatcagg tggtggatgc actacctgag
     1441 gctgtatcgc gtcgttcgct gggattacca gcggaaaaaa tctgctcggt gtaccgcgaa
     1501 agcgacatcg taccgggaga gcagaccgcc accagcatat tgaaacaacg cacaaaaaat
     1561 cttgcaccgt tgccttacgc ccaccagcaa caaaaatcac cacaggaaaa gacggtggta
     1621 agcatcaccg ttgatccaga gtctccggaa tctttcatga agctgcctaa acgtcgccgc
     1681 tgggttaagg agaaatacac acgttgggtt aagacacagc cgtgtgcttg ctgcggtatg
     1741 ccagccgacg atccgcatca tctgattggt cacgggcagg gcggaatggg aacaaaagca
     1801 catgatctct ttgtgttgcc tttgtgcaga aagcatcaca acgagctgca tacggataca
     1861 gtggcatttg aagataagta tggctcccaa ctggagctga tatttcgttt tatcgatcgc
     1921 gcgctgacaa ttggcgtact ggcgtaagtg gagaacgagc atgaaccttg aagccttacc
     1981 aaaatattac tccccaaaat ctccaaaatt gagcgatgac gctccagcga caggcaccgg
     2041 ttgtttaaca attacggatg taatggcagc gcaggggatg gtgcagtcga aagcaccact
     2101 tgggttggcc ttatttctgg caaaagttgg tgttcaggac cctcagtttg cgattgaagg
     2161 cctgctaaat tacgcgatgg cactggataa cccgacattg aacaaattga gtgaagaaat
     2221 ccggttacag attattcctt acctcgtgag ttttgccttt gctgattact ccaggtctgc
     2281 ggcaagtaag gctcgctgtg agcattgttc aggtacggga ttttataatg tattgcgcga
     2341 agtggtgaaa cactacagac gcggggaatc tgtaatcaag gaagaatggg tgaaggaact
     2401 atgtcagcat tgccatggta agggcgaagc cagcacagcg tgcagagggt gtaagggtaa
     2461 agggattgtt ctggatgaaa aaagaacccg gtttcatggc gtaccggtat ataagatttg
     2521 tgggcgttgt aatggaaacc ggtttagtcg tttaccgacc acgctggcac gacgtcatgt
     2581 ccagaagctg gtaccagacc tgaccgatta tcagtggtat aaggggtatg cggacgtcat
     2641 tggtaaactg gtaacaaagt gctggcagga agaagcatac gcggaagcgc aattgaggaa
     2701 ggtgacgaga taaatgattt ttgctgaaga tggcgacatg atgtttgcat ttttcaaaaa
     2761 atatggataa aattttttca acgatgggct ttgtataccc gacgttaaga aaaagtagaa
     2821 aacccgctga tgagcgggtt ttgtgcttta aatggggcaa tggtaatgtt gaatctcatc
     2881 ccgggactca tgtctgttaa cttattattt agctggtgac ttggttattt gcctgatgtt
     2941 taaaatgttt tcttccagta caatgtccct aaacacaatg agtctgctta ttatattatt
     3001 agcagagcta ttacggccaa agtacagcat aagcttttaa agccaatcaa ccagtcatca
     3061 agacagacgg ggttattcat aaaaactctc catgtgtgat ccgatggggc ctgaaattaa
     3121 agctttaata tagctcatga aaggtaaaca ttggcagctg aagggccacg cagaccattt
     3181 atccggcaaa attccacgcg taatccggtg gtaatttctt ctgcatcgcg gagattgagc
     3241 gctgaaacat gaagctggac atcgatacga ccatcggatg gggtgataag acccttgccg
     3301 cttttgccgt caaaggtttt gacaattcct gtcattttac gggacaaaaa aattccttaa
     3361 tactgataac ttggcgcact atacacacgt tcctgaagaa agctatagtt ttttgatggg
     3421 gttgaagatg gctggatgtc taaaataaac attgcttcat atgttcaact atgcgttaat
     3481 gattgcgtcg gtttgaagaa cagacgatat acgaagtagt ttactaaagc agttctcatt
     3541 tcaggtgtta ttcacttatt ccttctttga gtctctccaa ttaagtacga agtcgtttct
     3601 gttatgcaaa ccatttatgc cgaaaggctc aagttaagga atgtagaatg tcaaataaaa
     3661 tgactggttt agtaaaatgg tttaacgctg ataaaggttt cggctttatt tctcctgttg
     3721 atggtagtaa agatgtgttt gtgcattttt ctgcgattca gaatgataat tatcgaacct
     3781 tatttgaagg tcaaaaggtt accttctcta tagagagtgg tgctaaaggt cctgcagcag
     3841 caaatgtcat cattactgat taaaattcat cgctcgtctg tatacgataa cgaagaaggc
     3901 tgatgcctga gtagagatac ggacagagta gtgaatattg gatctcttta ataaaaagta
     3961 aggaggtcca atacatgaaa caatggctag catatttggc aaaatcttaa tcaggaaaag
     4021 tatgctaacc attgtggtga agtgcaggtt tgctgcatga atagttttac agcagaagct
     4081 aactgctggc atggcaaaac aaagtgcgta agtggatgac tcccacaaaa agcaccacaa
     4141 tctcaaaccc gctc
//
LOCUS       DAPOZO010000196         4107 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPOZO010000196 DAPOZO010000000
VERSION     DAPOZO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.197"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..2834)
                     /locus_tag="ROI21_004699"
     CDS             complement(<1..2834)
                     /locus_tag="ROI21_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDX5113515.1"
                     /translation="MNRISRYYFHRSVLSLLIAAMIYAPPGMTAFTSNVIGVVNDETV
                     DGSQRVDERGTTNNAHIINHGNQEVYGGISNGSVIDTGGHQEVSGHGSYQGQANNTVI
                     NGGSQTISEGGISTGTIINDKGTMSVLTNAKADATRIDNGGAMDVAGNATNTIINGGT
                     QNIYNHGIATGTNINSGTQNIKSGGKADTTNISSGSKQVVEKGGTATGSNIRAGGTLI
                     VDTGGIAHGVYLDTGSALVANTGAGTDIDGYQRSSHFTITGGRAEHVVLENTGQLTVV
                     AQTSAVDTIVDAGGKLIVHEEAVAYTTRLNNGGILDVREKGSATGIQQSSQGALVATT
                     RATRVTGTRADGVAFSIEQGAANNILLTNGGVLTVESDTTSAKTQVNAGGREIVKTKA
                     TATGTTLTGGEQIIEGVANETTINDGGIQTVSANGEAIKTTINEGGTLTVNDNGKATD
                     IVQNSGAALQTSTANGIEISGTHQYGTFSIASNLATNMLLENGGNLLVLAGTEARDST
                     VDKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGTAIVYAGTLAD
                     ASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATFTIGNGVDTTLADLTAASRGSVW
                     LNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAPATTNGIYNTLTTSELSGSGNFYLHTN
                     IAGSRGDQLVVNNNATGNFKIFVQDTGISPQSDDAMTLVKTGGGDASFTLGNTGGFVD
                     LGTYEYVLKSDGNSNWNLTNDVNPNPNPNPNPNPNPNPNPNPNPNPNPNPNPNPNPNP
                     NPNPNPNPNPNPNPNPNPNPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAEL
                     NSIRERLNIMKASPHNNNVWGATYNTRNNVTTDAGAGFEQTLIGMTVGIDSRNDIPEG
                     IATLGAFMGYSHSHIGFDRGGHGSVGSYSLG"
     gene            complement(2899..3018)
                     /locus_tag="ROI21_004700"
     CDS             complement(2899..3018)
                     /locus_tag="ROI21_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDX5113516.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2979..3164)
                     /locus_tag="ROI21_004701"
     CDS             complement(2979..3164)
                     /locus_tag="ROI21_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113517.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            3265..3438
                     /locus_tag="ROI21_004702"
     CDS             3265..3438
                     /locus_tag="ROI21_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113518.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3440..3784
                     /locus_tag="ROI21_004703"
     CDS             3440..3784
                     /locus_tag="ROI21_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDX5113519.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3788..>4107
                     /locus_tag="ROI21_004704"
     CDS             3788..>4107
                     /locus_tag="ROI21_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDX5113520.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRA"
BASE COUNT          974 a         1012 c          984 g         1137 t
ORIGIN      
        1 cccagagaat aactgcccac actgccatga cctccgcgat caaaaccgat atgtgaatgg
       61 gaataaccca taaaagcgcc cagcgtggca attccttctg gaatatcatt acggctgtcg
      121 atccccactg tcattccaat cagcgtctgc tcaaacccgg cccccgcatc ggtggtgaca
      181 ttattacggg tgttatacgt cgccccccag acattattgt tgtgtggact cgctttcatt
      241 atgttcaacc gctcgcgaat actgtttagc tcagcatcaa ataccaacgg taatgttgct
      301 gccatattga gtacagctgc cgtagaaggc gtgatgcgtt tctccggaac gggtgtcggc
      361 gttggatcgg gagtcgggtc tggatttgga tttggatttg gatttggatt tggatttgga
      421 tttggatttg ggttcgggtt cgggtttggg ttcgggttcg ggttcgggtt cgggttcggg
      481 ttcgggttcg ggtttggatt tggattcgga tttgggtttg gatttgggtt tgggttcgga
      541 tttgggttga catcattagt caggttccag ttgctgttgc cgtcgctttt caggacatac
      601 tcataggtcc caagatcaac gaaaccgccg gtattgccca gcgtaaacga cgcatcccct
      661 ccccctgttt tcaccagcgt catcgcatcg tcagactgag ggctgatgcc ggtatcctga
      721 acaaagattt taaaattacc agtggcattg ttgttgacga ccagttgatc gccccgggag
      781 cctgcaatgt tggtatgcag gtagaaatta ccgctgccgg aaagttcact ggtggtcagc
      841 gtattgtaga taccgttagt tgtggcaggc gctgataaat aaacatcacc gtcgttgagt
      901 agcaaactgt ttactctata ttcgcagttg ctggtgcctg cacaggaatt attgctgtta
      961 agccagacac tgccccggct ggcagccgtc aggtcggcaa gcgtcgtatc aacgccattg
     1021 ccgatagtga atgtcgcgct atcggtaatc cggatcgccc cttcgagttt aactggcgta
     1081 acattatccc gtggcgtcat taacgacagg cttcctgtcg cgccactgac cgatgcatcc
     1141 gccagcgtac ctgcgtagac gattgctgtc ccgccagcaa cctggagatc ttctgcccgg
     1201 gccagagcct gaaactcatc ttttgaccgc ccaagggtat attgcccacc agagttaacc
     1261 tttgtggcgg agtcctgacc caggttttgc attgcaccac ccttgtcaac cgtggagtca
     1321 cgggcttcgg tacctgctaa taccaataaa ttaccgccat tttccagcaa catattggtc
     1381 gctaaattgc tggcaatgga aaaggtgccg tactggtgcg taccgctgat ttcaataccg
     1441 ttagccgtgc tcgtctggag agcggcaccg ctgttctgga cgatatctgt cgctttgcca
     1501 ttatcgttga ctgtcagcgt tccgccttca ttgatcgttg tttttattgc ctctccgtta
     1561 gctgaaactg tttgtattcc gccgtcgtta attgttgtct catttgccac accctcgatg
     1621 atttgttcac cgccagtgag cgtcgtgcct gtcgcagtgg cttttgtttt gacgatctcc
     1681 cgaccgcccg cattaacctg tgttttggcg gaagtggtgt ctgactccac ggttaacacg
     1741 ccgccatttg tcagcaggat attgttcgcc gcaccctgct cgatgctgaa cgcgacgcca
     1801 tcagcgcgtg tccctgtgac ccgcgtcgcc ctggtggttg caaccaacgc gccctgacta
     1861 ctctgctgta tccccgttgc gctgcctttc tcccgcacat cgagtatgcc gccattgtta
     1921 agtctggtgg tatatgctac ggcctcctcg tggacaatca gcttaccgcc agcatcaacg
     1981 atggtatcta ccgccgaggt ttgagctacg accgtcagtt ggccggtatt ttccagcaca
     2041 acatgctcag ccctgcctcc ggtaatagtg aagtgagatg acctctggta tccatcaata
     2101 tcagtccccg cgcccgtatt ggcaactaaa gcgctccccg tgtccaggta aaccccatgt
     2161 gcgataccgc cggtatcgac aatcagcgtg cctccggcgc gaatattgct gccagttgcc
     2221 gtacctcctt tctcaacaac ctgcttgctc ccggaagaga tatttgttgt gtcagctttc
     2281 ccgccgctct tgatattttg cgtgccactg ttgatattgg ttcccgtagc aataccatga
     2341 ttataaatat tctgtgtacc gccattaatt atggtattag tcgcgttccc ggcaacatcc
     2401 atggccccgc cgttatctat tcgggtcgca tcagctttag cattcgttaa aactgacatt
     2461 gttcctttat cgttaattat cgtccctgta gaaattcctc cctcactaat tgtctggctg
     2521 ccaccgttaa tgactgtatt attagcctgc ccctgataag agccatgacc agatacctct
     2581 tgatgtccac cagtatcaat aacacttcca ttagaaatcc cgccatatac ctcctgattg
     2641 ccgtgattga tgatatgagc attatttgtt gtacctcgtt catctactct ttggctgccg
     2701 tctacagtct catcatttac aacgccaata acattagaag tgaaggccgt catcccaggc
     2761 ggggcatata tcatggcagc tatcaataag gaaagtactg agcggtgaaa ataatagcga
     2821 ctgattctgt tcataaattt catcctctga aaaatgaata ctgagtagcg tctaagtaac
     2881 ctaagctcgg ctgcaacatc agcccgcagg caccagacca ggggattcat cctgaagaga
     2941 cagcgcaggt gtattgtgtt caccgctcac caaagacatc atgatgaaat gatgatatcc
     3001 cgcataagaa agaggcattt tttaaacgca gtgcgctgaa gtgtagttgg ataaaaaatc
     3061 actcttttca gataatactg tcgtacgctt atctttcgac aatgaggccg tcggcaaaat
     3121 aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc tcacataaaa atcacacaat
     3181 aatattgata aatcagatta tcttaccttt acaacattgc gttaacagtc aatgacactt
     3241 cttgaataaa aatattgtca atcaatgagt tattcctcat ttaagattat tctcattcac
     3301 gtaaaaaaca taattcctat catcacagcc acgctcattt tgaattatct gaataatagc
     3361 gagcgttctt tagttaagca gatcttaatt gaagacgaaa ttattgtgtg tgcaacttac
     3421 ttaataccgg acatctaagt tgaagaaaaa aatacttgcc ttcgggctta tctcagcatt
     3481 gttctgctcc acgccagcga tggcggatat gaaccgcacc acgaaaggtg cattgctagg
     3541 cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc gtactgaaag gtgcagctgt
     3601 tggcgctggt gttggtgcag taacagaaaa aggccgcgac ggtaaaaatg cgcgtaaagg
     3661 tgcaaaagtg ggtgccgccg ttggtgctgt cactggcgtt ctgaccggca atggcctcga
     3721 aggggcgatt aaaggtgccg tgattggcgg tacgggtggt gcaattctgg gtaaaatgaa
     3781 ataaggaatg atgatgaaaa aatttgcttt actggcaggt ctgtttgttt tcgcaccaat
     3841 gacctgggcg caggactaca atattaaaaa cggtttgccg tcagaaacct atattacttg
     3901 cgcagaagcc aacgaaatgg caaaaactga cagcgcacag gttgcagaaa ttgttgcggt
     3961 aatgggtaat gccagcgttg ccagccgtga tttaaaaatt gagcaatctc cggaactgag
     4021 cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa gacccgcaaa tgctgttgat
     4081 cacggcaatt gatgacacca tgcgtgc
//
LOCUS       DAPOZO010000197         3905 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPOZO010000197 DAPOZO010000000
VERSION     DAPOZO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.198"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(40..144)
                     /locus_tag="ROI21_004705"
     CDS             complement(40..144)
                     /locus_tag="ROI21_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113521.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            198..2183
                     /gene="pdeR"
                     /locus_tag="ROI21_004706"
     CDS             198..2183
                     /gene="pdeR"
                     /locus_tag="ROI21_004706"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDX5113522.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESKKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            2330..2503
                     /locus_tag="ROI21_004707"
     CDS             2330..2503
                     /locus_tag="ROI21_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113523.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            2593..3342
                     /locus_tag="ROI21_004708"
     CDS             2593..3342
                     /locus_tag="ROI21_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDX5113524.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            3611..3829
                     /gene="osmB"
                     /locus_tag="ROI21_004709"
     CDS             3611..3829
                     /gene="osmB"
                     /locus_tag="ROI21_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDX5113525.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         1019 a          881 c          933 g         1072 t
ORIGIN      
        1 tatttccccg ctccaccttc gtcatatccc ggcagatttt taactgactt tcgtttgaaa
       61 actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaaacaa tgcgtttagc
      121 cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac tccgctgtta
      181 accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac aactttctcg
      241 gatcgcacaa tccatactgg cggttaacgg aaagcagcga tgttttgcgc ttttctacaa
      301 ccgaaaccac agaacctgaa cgtacgttgc agttatctgc cgaacaggct gctcgcatca
      361 gggaaatgac agtcatcact tccagtctga tgatgagtct gaccgtcgat gaaagcgatc
      421 tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc aacgcgtcgg
      481 catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc tttgctgagc
      541 aggtagtttc tgaagcacat tccgcaatag tgattctcga cagtcggggg aatatccagc
      601 gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc attgggcaaa
      661 gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc aataaccgtg
      721 tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca cgtaaaggcc
      781 agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa aacgagattt
      841 ttttaatctg ttccggcacc gacattaccg aagagcgccg cgctcaggag cgactgcgca
      901 ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg caggagttaa
      961 tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat cttgatttgg
     1021 ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag ctattacgcg
     1081 acgtgtcatt ggccatttta agctgtctgg agcatgacca ggttttggcg cgtccaggtg
     1141 gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa gcaatggcat
     1201 cacgaatttt gactcgctta cgactcccct tccgcattgg tttaattgaa gtttatacca
     1261 gctgttcagt gggtatttca ctctctcccg aacatggttc ggacagcgcg gctattattc
     1321 gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa ttttgcgttt
     1381 ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc aacttgcgta
     1441 aagcactgga aaacgatcag ttggttattc actatcaacc gaaaatcacc tggcgtggcg
     1501 aagtgcgcag tctggaagct ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
     1561 cgttggactt catttcctac gccgaagagt cagggctcat tgtgccttta ggccgttggg
     1621 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggtatt aacctgcgag
     1681 tggcggtaaa catttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
     1741 aggttctcca ggaactcaat tttgaatact gccctattga tgttgaactg acggagagct
     1801 gcctgattga gaatgatgaa ctggcacttt ctgttattca acaatttagc caactaggtg
     1861 cgcaagtgca tctggacgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
     1921 ttccgatcga tgcgattaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
     1981 tatcgcagtc gctagtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
     2041 tcgctgaagg tgtggagagt aaaaaggaag atgctttttt aaccaagaac gggatcaatg
     2101 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgttggtata
     2161 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgata tattccgtca
     2221 ttatatatcg tattactgga tgataattag ccgttgctgt taaacacttc tcttttagcg
     2281 gcaatttttg cattattcct tttccacttt tgctataagg atgtccttga tgtctgaatt
     2341 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
     2401 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
     2461 tgaatctacg ccagacatcc cgaaggcacc gtgggagatt taacaatcgt gccttcaggt
     2521 tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta taacgttata
     2581 agaattacag cgatgaattc ccgacaacaa acaattctac agatggtcat tgaccagggt
     2641 caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag
     2701 gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtatcg
     2761 cttgatagtg atgacgtcga aactcgtatg atgagcaact atacgctgaa gcgtgaactc
     2821 gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga aaatggcagc
     2881 agcaatgccc tgctcgcccg gaccctgggc gagcagaaga aaaatgtcac tatcatcacg
     2941 gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagttat tttgctcggt
     3001 ggcgtgtacc agaaaaaaag cgaaagtatg gttggtcctt tgacacgcca gtgcatccaa
     3061 caggtgcatt tcagcaaagc atttattggt attgatggct ggcaacctga aactgggttt
     3121 accggtcgcg atatgatgcg taccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa
     3181 gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggcccc
     3241 gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat
     3301 cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg
     3361 gatgatttgc tgttttgcaa atcatccgct ctaagatgat tcctggttga taattaagac
     3421 tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata taacaggaag
     3481 tcattatcac ctgcgtgata taaccctgcg cgcgagcgga tttcacggaa taatttcacc
     3541 aggcttattc ttagctatta tagttatagt gagcttactt ccgtgaatca taaattcagg
     3601 agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg caattaccct
     3661 ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gatcgcaaca ccgcaatcgg
     3721 cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt tgggtacatt
     3781 aggtggcgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat cgtaaataaa
     3841 tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga aataactgaa
     3901 taata
//
LOCUS       DAPOZO010000198         3892 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPOZO010000198 DAPOZO010000000
VERSION     DAPOZO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.199"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(39..950)
                     /locus_tag="ROI21_004710"
     CDS             complement(39..950)
                     /locus_tag="ROI21_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HDX5113526.1"
                     /translation="MGRTKSPMSTDLNAEVPLSDDLNISLNTMAQHRVEIMQQFGDGL
                     PYERDRIVHEARFYMAQSAEAMLEAGKRLIILKENEPHGDFINIVESELSMSKRTAQV
                     MMQASIKYLSPQLESKAQTFALLGKAKLFELMTEDDENLAELADGGTVAGLTLDDVDR
                     MSVRELRQALREARETNAAQQRVLADKNEKIDSLSTRLEKKSRIQPPEPDEEVKKLRA
                     EVTALAVEAESAIAVRLSSAFETLCAYCAENMIDTPRDFMAGLVCQLESTARSLRSTF
                     DLPDEPTGNAAPSWLTEPTPQINGLEA"
     gene            complement(961..1269)
                     /locus_tag="ROI21_004711"
     CDS             complement(961..1269)
                     /locus_tag="ROI21_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5113527.1"
                     /translation="MNKPNTSSAGTRILRVLKALRGHALNGISNGELAAALHESPANI
                     NRALNTLIEEGLALKLENGRFAPGIQLLQIAMAHSNEMARAQDRINEINQRVISGSRL
                     "
     gene            1579..1995
                     /locus_tag="ROI21_004712"
     CDS             1579..1995
                     /locus_tag="ROI21_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5113528.1"
                     /translation="MNNKDVNAVLSRLMSLFQAENDSELARALNVNRQTLASWRKRDS
                     VPYAICINIAEEKGVSLDWLLTGRKQGLSKEVDVTPTQVQFSQADLTMLELLKQLDPE
                     VRNDLLRGAAEKQRVIEMEKQLKELSATIERLKNVG"
     gene            2118..3011
                     /locus_tag="ROI21_004713"
     CDS             2118..3011
                     /locus_tag="ROI21_004713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113529.1"
                     /translation="MSGDVRNYRIEFHQLSFKATDQLANLGDAFQSVIDKQSPSSSNH
                     EGFTREIWGLKKRADGSFSGELRKFRNSNIPDKGAPGKQSTPIPLAKDEGIIERNFFV
                     FYPRINVIGWHYNHMACGISRFSAFLSTIFGSKVKPAPLLEKDAIARLMRGDVKMKKI
                     QLTIPRPRNPEFYPDTDFSQKTLQLLGDAGADTMQIVMGINTRRGDSDGRLKQNIKFA
                     MQELVEFGAKKAVIDVFDADGVVHPVDLIAERISSYQSVVTDARFPPAETMYRLIDTA
                     YAENRESINAYIGEDELGPIA"
     gene            2971..3408
                     /locus_tag="ROI21_004714"
     CDS             2971..3408
                     /locus_tag="ROI21_004714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113530.1"
                     /translation="MHILEKMNSALLLSLLIGCGIGFLYWFKLPHMPVAAIPNFMTTL
                     SSVFATLLGFIITAIALLASLLDKPLLKNMQKTGHYKRLMTDAFYTCLVLLVLVLTCL
                     IGLMLQSKHQEIVAAILIGLVVMSMLCLLQTGRRLFNIIKVIS"
     gene            complement(3461..>3892)
                     /locus_tag="ROI21_004715"
     CDS             complement(3461..>3892)
                     /locus_tag="ROI21_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDX5113531.1"
                     /translation="QKQAFGGKVAEHSFGTSTTSPPRFNLLRIEEELSAAHLRLSRTV
                     IEHMDWQQCIERYDRPHTLFYCDPPYLGTEGYGVDFPEGNYSRLAELARCIRGKMIIS
                     VNDIPEMRQVFNGLNIHCVKVNYSLAGKGTLRRELIVSNLE"
BASE COUNT          997 a          870 c          911 g         1114 t
ORIGIN      
        1 acataaacca gtcgttcagt cagggcagca ttcattggtt atgcctccag cccgttaatc
       61 tgtggcgtcg gctcagtcag ccatgaaggc gctgcattgc ctgttggctc gtccggcagg
      121 tcaaatgtgg agcgcaggct acgcgctgtg ctttccagtt gacagaccag gcctgccatg
      181 aagtctctgg gggtatcaat catgttttca gcacaatatg cgcacagtgt ctcaaaagcg
      241 ctggacagtc ggacggcgat agcagattcc gcctcaaccg ctaatgctgt cacttccgcc
      301 cgcagcttct taacctcctc atcaggctca ggcggctgaa tacgggattt cttttccagt
      361 ctggtagaga gagaatcaat tttttcattt ttatcggcga gtacgcgctg ttgtgctgcg
      421 ttggtttcgc gcgcttcgcg cagggcctgg cgaagttcgc gtactgacat gcgatcaacg
      481 tcgtcaagcg tcaggccggc aacagtgcca ccatcagcca gttcggcaag gttttcatcg
      541 tcttccgtca ttaactcgaa taatttggct tttcctaaaa gcgcaaacgt ctgcgctttt
      601 gactcaagtt gtggtgataa atattttatt gatgcctgca tcattacctg tgcagtacgc
      661 tttgacattg ataattcaga ctcgacaata tttatgaaat caccatgtgg ttcattttct
      721 ttcagtatta tcagtcgttt acctgcctct agcatggcct cagcactttg tgccatataa
      781 aagcgtgctt cgtgaacaat gcgatctcgt tcataaggca gaccatcgcc gaactgctgc
      841 atgatctcca cgcggtgttg tgccatggta ttaagactga tgttgagatc atcagacagc
      901 ggcacctcag cattcagatc agtggacatt ggtgatttgg tacgtcccat tgattactcc
      961 ttataaacga ctacctgaaa tgacacgttg gttgatttcg ttaatacgat cctgcgctcg
     1021 tgccatctcg ttactgtgag ccatggcgat ctgcagtaac tggatgcccg gtgcaaaacg
     1081 tccattctcc agtttcaggg ccagcccttc ttcaatgagg gtgttgagtg cccgattaat
     1141 gtttgctggt gactcgtgca gtgctgccgc cagttcaccg ttggagatac cgttcagagc
     1201 gtggccacgt aacgccttaa gaacacgcaa aatacgggta cctgcgcttg atgtatttgg
     1261 tttattcatg acacctcctt aaggataccg ggggtaactt ctttacctat tgcaaccgat
     1321 agatctcgca aaatccgata agtcaaacga ccacgtggta gttcactttt accagcccag
     1381 cgactgactg cctgtgtgac tgttcgtggc tcatagcctg cgttaagcgc gaactggcgc
     1441 aagctactgc ctcgttcaac tagtcgtgcc cgaacttgtt gtttattcat atgcggatag
     1501 ttccagttgg gttatcatgt actcaatatg aataaatgta cttatccaaa ataaataaat
     1561 caagtggaat tacgcaaaat gaataacaaa gatgttaacg ctgtgctttc ccggttaatg
     1621 tcactattcc aagcagagaa tgatagtgag ttggcaagag cgcttaacgt caataggcag
     1681 acacttgcaa gttggaggaa gcgtgattct gtgccttatg caatttgcat aaacatcgca
     1741 gaagaaaagg gggtttcttt agattggttg ctaacaggta gaaaacaagg tttatcaaaa
     1801 gaagtagacg taactccgac gcaagtgcag tttagccaag ctgaccttac tatgcttgag
     1861 cttttaaagc agctagaccc cgaggttcgt aacgatcttc tgcgaggcgc tgcggaaaaa
     1921 cagcgagtga tcgagatgga aaaacaactg aaggaattat ccgcaacgat tgagcggtta
     1981 aaaaatgtgg gttaatctat tcccaatgag aacaattgac aaggttatca aacctcttcg
     2041 ttatatgcgc tgatttaaac agtaggatgt gctgtttgca ctaaccatct gaactttccg
     2101 cgttaaggat taatcgtatg agtggtgatg tgagaaatta tcgtattgag tttcatcagc
     2161 tatcgtttaa ggccacagat cagctggcaa atctcggaga tgcttttcaa tctgtaattg
     2221 ataaacagag cccttcctct tcaaaccatg agggttttac tagagaaatt tgggggttga
     2281 aaaaacgcgc ggatggttct tttagtggag agttgagaaa atttcgcaac tcgaacattc
     2341 cggataaagg tgcaccaggg aaacagtcta cccctattcc acttgccaag gatgaaggga
     2401 tcattgagcg caatttcttt gtattctatc ctcgaatcaa tgttataggt tggcactata
     2461 atcatatggc ttgtggtata agccgtttta gtgcatttct ttcaactatc tttggttcaa
     2521 aggttaagcc cgcacctttg ctggaaaaag atgcaatcgc taggcttatg agaggagatg
     2581 tcaaaatgaa aaaaatccag ttgacgattc ctcgcccccg taatcctgag ttttaccctg
     2641 atactgattt cagtcaaaag acactacaac tgcttggtga tgcaggtgcc gatacaatgc
     2701 aaattgtaat ggggattaat actcgccgtg gcgatagtga tggtaggctt aagcagaata
     2761 taaaatttgc tatgcaagag ttagtcgagt ttggggcgaa aaaggctgta attgatgtgt
     2821 tcgatgctga tggtgttgtc catcctgtgg atttgattgc cgagcgtatt tcgtcctatc
     2881 aatcggtagt tactgacgct cgtttccctc cagccgaaac catgtaccgt ctaatcgata
     2941 ctgcgtatgc tgaaaaccgg gagagtatca atgcatatat tggagaagat gaactcggcc
     3001 ctattgctta gtttattgat tggatgtggg attggttttc tctactggtt caagttgccc
     3061 catatgccag tagccgcaat cccaaacttc atgactacat tgtccagtgt ctttgcaacg
     3121 ttgttaggtt ttattattac agcaattgca ctacttgcat cattgcttga taaaccgtta
     3181 cttaaaaaca tgcaaaaaac cggacattac aagcgtctga tgaccgatgc attctacact
     3241 tgcttagttt tgctagtgtt ggtgttgaca tgtttgattg ggttgatgct gcaaagtaag
     3301 catcaagaga ttgtagccgc tatcctcatt gggcttgttg ttatgtctat gttgtgttta
     3361 ttacaaacag gtaggcgatt attcaatata atcaaagtga tatcataaca atgcaccatc
     3421 aggtgcattg ttatgcaaga ctacttacct ccttctgagc ctactctaaa ttacttacaa
     3481 taagttcgcg ccgtagtgtt ccctttcctg caagactgta gttgaccttc acacagtgaa
     3541 tgttcagacc gttaaaaacc tgccgcattt ccgggatatc gttgaccgat ataatcatct
     3601 ttcctctgat gcaacgggcc agctctgcca accgcgagta gttcccttca ggaaaatcca
     3661 caccatagcc ttccgtaccc aggtacggtg ggtcgcagta gaacagcgtg tgcggacggt
     3721 cataacgctc aatgcactgt tgccagtcca tgtgttctat caccgttctg gaaagtcgca
     3781 ggtgtgccgc tgagagctct tcctcaatac gcagcaggtt gaagcgtggc ggggatgtgg
     3841 tggaggtgcc gaaactatgc tctgccacct tgccaccaaa cgcctgcttc tg
//
LOCUS       DAPOZO010000199         3797 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPOZO010000199 DAPOZO010000000
VERSION     DAPOZO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.201"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            393..599
                     /locus_tag="ROI21_004716"
     CDS             393..599
                     /locus_tag="ROI21_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDX5113532.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            685..1257
                     /locus_tag="ROI21_004717"
     CDS             685..1257
                     /locus_tag="ROI21_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113533.1"
                     /translation="MQKLENRCDLLLTQHQKWMASVTRFIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPATPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGESRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELESWAVARKREKRELELMIDEYLLYAC"
     gene            1466..1606
                     /locus_tag="ROI21_004718"
                     /pseudo
     CDS             1466..1606
                     /locus_tag="ROI21_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2751..3770
                     /locus_tag="ROI21_004719"
     CDS             2751..3770
                     /locus_tag="ROI21_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDX5113534.1"
                     /translation="MNNEQMLIKCKDPRKCRFYAEYLNHKYPNEFPQFAPDNPQNCIR
                     NVNSFLKEKYYKASNNSFGVFGSTAEEALSKEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
BASE COUNT         1236 a          846 c          698 g         1017 t
ORIGIN      
        1 tgagtttgag ctcatgtatt tgatctctct tatatattta taataccaat cagacagtct
       61 tatcccacca gtgatatctg tcgcctgccc acaggtcaca ggctgactca ccatcaccgc
      121 cttcaaaaac aactctgcaa ttatcagaac gccagttttc cccttgtctc acgaaagtcc
      181 tacctccttt atcgcgtgcg gaagcatcgt ttcgcaacaa tcacccgaca taccggtacg
      241 gctcagccac cgcaatcccc ccgtttatct tctgacggtc acattcccga ttatttttta
      301 cgtttttccc cggttgttat gccggggaag gtggtgcgtt gttttcatca ccaacgccgg
      361 ttgagattaa caacatccgg aggaacattt tcatgaatac cccagtttcg ctgatggatg
      421 accagatggt cgacatggcg tttatcactc aactgaccgg cttaaccgat aagtggtttt
      481 acaagctcat caaggatggt gcctttccgg cccccatcaa actcggccgc agctcccgat
      541 ggctgaaaag tgaagtggaa gcctggttgc aggcgcgtat tgcacaatcc cgtccgtaat
      601 tcctgaccat tacccgttca accgcagcga atacactatc cggcctccgg aggcattttg
      661 ctgcgtgcta aaggagagaa tattatgcaa aaacttgaaa accggtgtga tctgctgctc
      721 acccagcatc agaaatggat ggcaagtgta acccggttta ttgtcgctca cggaatgggc
      781 tctccgcatc ttcatggtta tcatcgcctg accctggccc acttttttct gccggaaaaa
      841 ggcaccatca tctcggtggc accacaggga ctctatcagg tagtcaaccc ggcaacgcca
      901 ccgttcattc ctgccattca ggaagggtta atgaccagca ttcagactca tgaaatcatg
      961 ttacttacac attttaacct gggtggtgtt ttactcagtg aactacatcg tctgggagaa
     1021 agccgactgg caaacaggct gaacagtctg ctccggcgtt ttgacgatcg cgatctctac
     1081 cacaccctga tatggctgtg ctggtatgat ctgatgtgcg cccactccat gcagccctgg
     1141 actgaagagt taaagcataa atcacacgca gaacttgaaa gctgggctgt ggcgcgcaaa
     1201 cgggaaaagc gcgagcttga actgatgatt gacgagtacc tgttatacgc ctgttgattc
     1261 ataatcactc ccgggcggta cgtagtcagt gataccggaa tttccggaga gggtcgcagg
     1321 tatattgttt ctgtgcaatt catggttatt cacgttggat gtagtgattg ctgcatccaa
     1381 tgctatcgga aagagattaa ccagcaaatg gttcctcaaa tcgcattgat atttacccgg
     1441 cgcattgagc accaccatct gcccccgttc ccacatcaaa cggctggacc ggaaaacaat
     1501 ttgcttttca tgctccgtgg aaattcacga aaaagttatt gggtcattca tcgaaaaaac
     1561 atattctgct gactggagtc atcacccaga aataaacgtc aactgaacaa taacgatttt
     1621 ttctttgtgg gattccatac acttttccat ccccctctga aaattagtca tcaactcacc
     1681 aacatatctt ctgaaatttg tattaaactt attgcttaaa tagatgatat aatatttctt
     1741 ccattgcacg gaatcagcaa aaactgtcat tctggcaatc ttaacaagaa catttatcgg
     1801 ttaattttat cagtaaacaa aggaatatca tactgataaa atgaacattc agggtaagat
     1861 gatgaaattc acatgaaaaa tgctgaagat tatttatttc atgcagataa acgataaaaa
     1921 acaagagagt atatttttca gtgtaagagt caattgttca tctccactga cgatcacggt
     1981 cagcatacaa acaaagtgag tcctcctgct tagtcattgc gtatcaaaaa ctaaaaaaca
     2041 aaagattctg caaacaacgt gacatttttt atgacatttc ccgaatacat gtatatcaat
     2101 aaacggtagt catcgttgaa ttaataagtc ttacagagct atacgtagct tataaaacca
     2161 ctcaacgacc atcatcacct gactatttag cgggagtatc gtctgtataa atccataaat
     2221 aataacacct gttattaaaa taagaatgct gtcagccgta aactattaac acaacacctg
     2281 tccactctat ctgttctttt aactcaaccg gcgtcgaaaa tatttcctga ttagtgcatc
     2341 atagtcttct tttttctttc tcaaccatca aaccaaacac aacattcaca accacacaat
     2401 aaaacaaaac caaaaacata agattaacaa tgccagtcaa caggaaaaca ctgaatattg
     2461 aggtgcgctg taacctggac agcgcatacg gcgcatgtga tcaacacagt ccattctttt
     2521 ctgtcatccc ccaaatgcag aacaaacaat cataaccaaa tgaatataaa agaaaatatg
     2581 atttttcacc atcataaaaa catcaatgta tctttatata aaaacactga taacatgaca
     2641 tagacattta aaaacataaa caaatcacaa caaaaacatt tgataatcac ttagtgacaa
     2701 aataaaattg agacgatttt gttataagag aggcgacatg gagtgagata atgaataacg
     2761 aacagatgct tatcaaatgc aaagatcccc gtaaatgtcg gttttatgcc gaatatctga
     2821 atcacaaata ccccaatgaa ttccctcagt ttgcgcctga caacccacaa aactgtatca
     2881 gaaatgttaa ttcgttttta aaagaaaagt attataaagc ttcaaacaat tcttttggtg
     2941 tttttggttc gactgcagaa gaagctctca gcaaagaaat taatgaactt gaaattattg
     3001 cagggcaggc aatactgcct gaagagagtt ttctctggtt gaaaactgac gaacttgcga
     3061 ctttctttac ctggactact atttatctgt cgaaggaaga ccatacaaaa ttcccgaaaa
     3121 taaaccataa ttcatcagga ataaaaaacg gaattgaagt caggaaaata cgaacagtta
     3181 ccgatgaaac gttaaatact tacaaaaaaa gtaaccttcc ggataatcca tcatcccata
     3241 acgaacgaat aaaagtaatc atttcatatt ttgactcctg ccattcttta attaataaaa
     3301 atgcaaaaag catatacctc gaggaactca gacaggcgtg gcttaaaaag aaaagcgctt
     3361 gtcaaaagtt taagtggctc cttcctgacg atgatgaaat gtgtcagtgg ttctggacaa
     3421 gactgaaaca aaaacaggat gaaaaacatc cagtacataa cccagtgacc cggtggttca
     3481 taccatcttc aacgaaagaa tattatcttg cctctcttgt cgcctttgct ttatggaaag
     3541 aagcgcctga taccattgaa ctgttcagaa cacgaataaa tcatgcctgg catcagaaaa
     3601 aacaaaagga caaatatcgg agtgatggta aaaaggccat taatatacat ataagaagca
     3661 atgtgaaaga aatgcttgat gagctcagaa ctgtttacgg tctgagaaca ggagagtttt
     3721 ttgaaatgct gattacagag aaatatcttg aacacaagcg aaggaagtaa ttcattacct
     3781 gttacattct aaataat
//
LOCUS       DAPOZO010000200         3745 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPOZO010000200 DAPOZO010000000
VERSION     DAPOZO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.202"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            105..326
                     /locus_tag="ROI21_004720"
     CDS             105..326
                     /locus_tag="ROI21_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDX5113535.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPCVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            406..774
                     /locus_tag="ROI21_004721"
     CDS             406..774
                     /locus_tag="ROI21_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDX5113536.1"
                     /translation="MSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSNELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            864..1241
                     /locus_tag="ROI21_004722"
     CDS             864..1241
                     /locus_tag="ROI21_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDX5113537.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            1238..1726
                     /locus_tag="ROI21_004723"
     CDS             1238..1726
                     /locus_tag="ROI21_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113538.1"
                     /translation="MKLSLTLEADSVNVQALNMGRIVVDVDGVNLAELINVACDNGYS
                     LRIVDESDRTSAERTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            1738..1935
                     /locus_tag="ROI21_004724"
     CDS             1738..1935
                     /locus_tag="ROI21_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113539.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESEIIFDNDEDKTDSAALLPC
                     IEQARQDIRTLRHLQLQHPNR"
     gene            2028..2894
                     /locus_tag="ROI21_004725"
     CDS             2028..2894
                     /locus_tag="ROI21_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDX5113540.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTGFRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGVHLITGQQADRVADLERMLHLFSGRPIPDNR
                     ENIIIRLDDHIQSVQGQECYEDEMFSIRYFKKGSAHITFRKPQLVDRLNDIIARHYPE
                     ALASTVNKSRKT"
     gene            2966..3229
                     /locus_tag="ROI21_004726"
     CDS             2966..3229
                     /locus_tag="ROI21_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDX5113541.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            3226..3552
                     /locus_tag="ROI21_004727"
     CDS             3226..3552
                     /locus_tag="ROI21_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDX5113542.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQL"
BASE COUNT          999 a          963 c          913 g          870 t
ORIGIN      
        1 gaacacggtc tgctttaacc cgtcaccgtc acaatcacct tcatatcact tcagtttctc
       61 tttctcagct gtttcttact ttcacattca ggaggactat tctcatgaaa atcatcaccc
      121 gtggtgaagc catgcgtatt caccgtcagc atcctgcatc ccgtcttttt ccgttctgta
      181 ccggtaaata ccgctggcac ggtagcacgg atacatatac cggccgtgaa gtacaggata
      241 ttccctgtgt gctggccgtg tttgcagaac gccggaagga cagttttggt ccgtatgtcc
      301 ggctgatgag cgtcaccctg aactgagtct ggaagggcat tcagaagagc agaattatcg
      361 ccaccaccgg accattctta accaattttc tgtgaggatt ttatcgtgtc agacacactc
      421 cccgggacaa cgcttcccga cgacaataac gaccgtacct ggtggggact gccctgcacc
      481 gtgacgccct gttttggtgc ccgtctggta caggaaggca accggttgca ttaccttgcc
      541 gaccgcgccg gtatcagagg gcggttcagc gacgcggatt cgtaccatct ggaccaggcc
      601 tttccgctgc tgatgaaaca actggaactc atgctcacca gcaatgaact gaatccccgc
      661 catcagcata ccgtgacact gtatgcaaaa gggctgacct gcgaagccga caccctcggc
      721 agttgtggct acgtttatat ggctgtttat ccgacgcccg aaacgaaaaa gtaactctcc
      781 agaataacct tctgctccgg cctggtgttt tcaccacgcc acttttccat ttttcatctc
      841 tgcatatcag gaaaatcttc agtatgaaaa cattacccga cacgcatgta cgggaggcat
      901 cgcgctgccc gtctcccgtc accatctggc agatactgct gtcccgactg ctggaccagc
      961 actacggcct cacactgaat gacacaccgt ttgccgatga acgtgtgatt gagcagcata
     1021 ttgaggcagg catttcactg tgcgatgcgg tgaactttct cgtggaaaaa tacgcactgg
     1081 tacgaaccga ccagccggga ttcagcgcag gaacttcgtc gcagttaatc aacagcattg
     1141 atattctccg ggctcgcagg gcgaccggcc tgatgacccg cgacaattac agaacggtaa
     1201 ataacattac ccgggggaag catccggagg cgaaacaatg aaactttccc tgacgctgga
     1261 agcagacagc gttaacgtgc aggcgctgaa catgggacga attgtcgttg acgtcgatgg
     1321 tgttaatctc gctgaactga ttaacgtggc ctgcgacaat ggctactccc ttcgtattgt
     1381 tgatgaatct gaccggacat cagcagagcg cacgccacca tttgctgccc tgaccggcat
     1441 acgctgcagt accgctcata tcacggcaaa ggacaacgcc tggttgtatt cgctgtcaca
     1501 tcagacaaat gacaccggcg aatcagaatg gattcatttc acaggtagcg gatatctgtt
     1561 acgtaccgat gcgtggtcat acccggtttt gcggcttaag cgcctgggcc tgtcaaaaac
     1621 gttccgccgt ctggttgtca cacttacccg acgttatggc gtcagtctca ttcatctgga
     1681 tgccagcgct gaatgtctgc cggacctgcc cactttcgac tggtaaccag gaacaacatg
     1741 aaatcattaa caacggaaac cgcactggat attctgattg cgtggctgca ggacaatatt
     1801 gactgcgaat cggaaattat ctttgacaac gatgaggata aaacggattc ggcagcactg
     1861 ctgccctgta tcgaacaggc gcgacaggat atccgtaccc tgcgtcatct gcagcttcag
     1921 cacccgaacc ggtgagtctg actcaatcat ctcaccacac ttcattcccc caacgtcagc
     1981 ctgaacaggg ctggcgtttt catttatctg caaaagggat ttcgattatg tctgaaatca
     2041 cagcctctcg tccggaagtg gtcaacgggc atacggacgt tatctgctca accagtatcc
     2101 gccacattct ggctgtacga aagagtgcgc tgctgcaaat cgacacactt atccggcaac
     2161 tggcagaaat ctcagcaatg acagaaagca tcggaggaaa aaccgcactg gactgggcga
     2221 tgaaacagga ttttcgctgc ggctgctggc tcatggagaa accggaaacc gcaatgaaag
     2281 ccatcacccg caatctcgat cgcgaaatct ggcgtgacct gatgcaacgt tccgggatgc
     2341 tgtcattaat ggatgcgcag gcccgtgata cagggttcag gtcactggag tacgataact
     2401 ttccggaaat cagtgaagcg aacattctga gcacatttga acaactgcac cagaataagg
     2461 atgaggtgtt tgagcgaggg gtgatcaacg tcttcagggg actgagctgg aattacaaaa
     2521 ccaattgccc ctgcaaattt ggcagtaaaa ttatcgtcaa caacctggtg aggtgggacc
     2581 ggtggggggt tcatcttatc accgggcaac aggctgaccg ggttgctgac ctggaaagga
     2641 tgctgcacct gttcagcggc aggcccatcc ccgacaaccg ggaaaatatc atcatccgtc
     2701 tggatgacca cattcagtcc gttcagggtc aggagtgcta tgaagatgag atgttcagca
     2761 tcagatactt taagaagggc tccgcacaca tcacgttcag gaagccacaa ctggttgaca
     2821 ggcttaatga tattattgcc agacactacc cggaagccct ggcaagcaca gtgaacaaaa
     2881 gccgcaagac ataaaagagg tattttacaa ttcgcgaata ttaactatta ttaattgata
     2941 gtaatcaatc ggcgagaaaa tcaccatgcc taccagtgta tccctcagtc cttactttga
     3001 aacgtttatc cgcgaacaaa tagaaagtgg tcggtataac aatacaagtg aagtcattcg
     3061 tgccggactt cgtgctctgg aagaacggga acagcagata aagcttgaat cactccagag
     3121 cgccgttacc gcaggcatca acagcggtga aagtaaaagt gctgaagagg tattcgggcg
     3181 actgacgcac aaatacaaaa agatggcaga aggtgaacag ccaatatgaa actgactgtt
     3241 tcaccattag cagaacacga tcttgaagct ataggtgact ggatcgctca ggataatcct
     3301 gtccgtgcaa tcagttttac cgaagagctt taccaacaat gtctgttgat agccgaatcg
     3361 ccagtactct acagagaaag acccgagtta ggggctggga tcagaggttg ttcgtatggt
     3421 cgttaccttc tgctgttcag ggtactggat actgaagttc gtatcgaacg tatcgttcat
     3481 ggttcccggg atatcaacag ggtacttgca gagccagacg acaaaccatc agatcatttc
     3541 tggcagctat aaacaaaatc tgtgcgttaa cgctccctaa cacaggtagc caggtatttt
     3601 ataaatctct gtattttgtt gttacttcga ttccgcgatt cagcatatcg aaccagacaa
     3661 aatgattaca acaaagcaca aaaaagaggg attatagcgt caaaagaatt cgaattgcac
     3721 atgggggcct cattggtggc ctcaa
//
LOCUS       DAPOZO010000201         3458 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPOZO010000201 DAPOZO010000000
VERSION     DAPOZO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.203"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..329
                     /locus_tag="ROI21_004728"
     CDS             <1..329
                     /locus_tag="ROI21_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HDX5113543.1"
                     /translation="NLFPATFSDGYVDIYGYHLPRPLHFGTQGEGMVGVRPEAITLSD
                     RGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDVGEQYYLEIHPYGMF
                     VLADAA"
     gene            complement(438..1370)
                     /gene="mmuM"
                     /locus_tag="ROI21_004729"
     CDS             complement(438..1370)
                     /gene="mmuM"
                     /locus_tag="ROI21_004729"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HDX5113544.1"
                     /translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
                     VENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
                     REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
                     GADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAG
                     YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
                     LADYLPQWQAAGARLIGGCCRTTPADIAALKARS"
     gene            complement(1357..2760)
                     /gene="mmuP"
                     /locus_tag="ROI21_004730"
     CDS             complement(1357..2760)
                     /gene="mmuP"
                     /locus_tag="ROI21_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methylmethionine permease"
                     /protein_id="HDX5113545.1"
                     /translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
                     AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
                     WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
                     VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
                     GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQAGVEKSPFV
                     LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
                     VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
                     QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDPAQRIALWCGLPFVALCYGAY
                     FLTQPRNAKQEPEHVAE"
     gene            2968..>3458
                     /locus_tag="ROI21_004731"
     CDS             2968..>3458
                     /locus_tag="ROI21_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5B family transposase"
                     /protein_id="HDX5113546.1"
                     /translation="MFVIWSHRTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTISRWLAEAGVMMTQGTLVDATIIE
                     APSS"
BASE COUNT          856 a         1050 c          894 g          658 t
ORIGIN      
        1 ccaacctgtt cccggcaacc ttcagcgacg gatacgttga tatctacggc tatcatctgc
       61 cgcgcccgct gcactttggt acacagggtg aagggatggt cggtgtgcgc ccggaagcga
      121 tcacgctcag cgatcgcggc gaagagagcc agcgctgcgt gatccgccat gtcgcctata
      181 tggggccgca gtatgaagtg acggtggaat ggcacgggca ggagatatta ttgcaggtca
      241 acgctacgcg tctgcaaccg gacgtcggcg agcagtatta tcttgaaatc catccgtacg
      301 gcatgtttgt tctggcggat gcggcataac gttttgtggt tttgtaggcc ggataaggcg
      361 cttgtgccgc catccggcaa tgcctgatgc gctgcgctta tcaggcctac aatgaacgat
      421 gtagacccga taaaccctca gcttcgcgct tttaacgcgg cgatatccgc aggcgtggtg
      481 cgacagcacc cgccaatcaa ccgtgcgcca gcggcctgcc actgcggcag ataatccgcc
      541 agctgcgcgc aatgttcacc gtgatgatgc caggttttgc tcacggcatc gtaatgctcg
      601 cccgagttcg gatacaccac cagcggcagc accgttaaac cgtgtaaatg ctgcaacgca
      661 gcggtggtgt tttccagcgc aatacagtta atgcctagcg ccaccacctg cggataaccc
      721 gccagcaacg caaccacgtc acgcagcggc gtaccgtcgc tcaggtgttc gctgtcgcgc
      781 agggtaaatg agaaccacgc ccgcgcacgc ggatatgcgg tcaacagctc ggccaacgcc
      841 tcaatctcgg aaaaattcgg cagggtttcg caggccagca gatcggcccc ggcatccagc
      901 aaggcttcca cgcgcgggcg atgaaacgcc tgaaatgcct caacgctaca atgataatcg
      961 ccacggtatt cagagccatc cgccagatac gcgccgtaag gcccgacgga tcccgccacc
     1021 agaagcgttc ccgcctgcgg gttctctgcc agatacgctt cacgggcttt acgcgccagc
     1081 tccacgcttt tgccaatcag cgctttcgac tgcgcttcat ccagacctcg cgcggcgaag
     1141 cccgccggcg tcgcctgata gctggcagtg atcgcgcatt gcgcccccgc ccggtagtaa
     1201 tcaagatgca cttcgcggat aagctccggg ttttctacca gcactttggc tgaccacagg
     1261 ctgtcggcta agttacaccc tcgcgcttcc agctccgtcg ccatcgcgcc atccagcagc
     1321 aggatatcct gtttatcaag aagagcgcgt aacggattat tctgcgacat gttctggctc
     1381 ctgttttgcg tttcggggtt gagtaaggaa ataagcacca tagcacaacg caacaaacgg
     1441 taacccgcac cacaacgcaa ttctctgcgc tggatcgaat gccagcccaa cacaggccac
     1501 caggcacagc acaaaaccta atactggcac cagcggatac cacggcgcgc gataatgtaa
     1561 ttcactcaat gccttacctt gttgcagatg acggcgacga aaaacaaaat gcgaggcgca
     1621 gatactcagc cacaccgcta ccaccgcaaa cccggagatt gccgacagcg caacaaatac
     1681 cgtgtccggg gccaccacgc tggaaaacag cgccagcaca ccaccgagca tactgaccga
     1741 cagcgccgtc agtggcacgc cgtttttcgt tactcgcgca aaacaggccg gtagcgtacg
     1801 ttcattcgac aacgaccaca gcatgcgccc ggaggcatat aacccggagt tcgctgcaga
     1861 aagaatagcc gtcaggatca cgaagttaaa aatatcagcg gcgtacggga tccctacttt
     1921 ctcaaatacc agcacaaacg ggcttttctc cacgcccgcc tgctgcatcg ggatcagcgc
     1981 tgccagcaca aacacggtgc cgataaagaa aataatcagt cgcgcgatgg tagtacgaat
     2041 cgctaccggg ataactttgc gcgggttttc cgtttcaccg gcggcaatgc cgataagctc
     2101 ggtacccgaa aaagcaaagt tcactgccac catagtcatc aaaatcggta agccaccgtg
     2161 cgggaaccag ccttctgccg tgatattact cagccccggc gcgggcgagc catcctgcat
     2221 cggaataaag ccgaaaatcg ccgccccacc gaggatgata aaggcgatga tagtgaccac
     2281 tttgaccagc gagaaccaga actccccttc ggcaaaaaag cgcgtggaga taacattcag
     2341 accaaaaata atcgcgcaga acaccacgca ccagacccat accggcacct gtggaaacca
     2401 gtactgcata cagaatccag cggcggtaaa gctcgaaccc agcgccacgg tccaggtcag
     2461 ccagtaaagc caggccacgg tatacccggt agccggacca agatagcgcg cggcataaac
     2521 gtgaaacgct ccggtctccg gcatcgcgac cgacagctcg cccagacact gcataaccag
     2581 ccagaccacc agcgcaccaa tcagataggc cagcagcgtt cccgccgctc cagtggtgga
     2641 aatgatgtac ccggtattga agaataatcc tgtgccaatc acgccgccca aggaaagcat
     2701 aatcaggtga cgcgttttca ttgtgcgctt cagtggcgca ttttgttgtg ttgtttgcat
     2761 ataaccttct aaacgtatgg acatctaaac atccaaagaa ggtttgttat accgcgtttg
     2821 ttaacagaat gcaaagtcaa ccctgcacca gacgcagatg cccgcgccgg gcgcttaata
     2881 gcgggtgggg attaataatc agatacaaaa agggccgcca gctcataacg cctccggaag
     2941 gtgcgaacaa gtccctgata tgagatcatg tttgtcatct ggagccatag aacagggttc
     3001 atcatgagtc atcaacttac cttcgccgac agtgaattca gcagtaagcg ccgtcagacc
     3061 agaaaagaga ttttcttgtc ccgcatggag cagattctgc catggcaaaa catggtggaa
     3121 gtcatcgagc cgttttaccc caaggctggt aatggccggc gaccttatcc gctggaaacc
     3181 atgctacgca ttcactgcat gcagcattgg tacaacctga gcgatggcgc gatggaagat
     3241 gctctgtacg aaatcgcctc catgcgtctg tttgcccggt tatccctgga tagcgccttg
     3301 ccggaccgca ccaccatcat gaatttccgc cacctgctgg agcagcatca actggcccgc
     3361 caattgttca agaccatcag tcgctggctg gccgaagcag gcgtcatgat gactcaaggc
     3421 accttggtcg atgccaccat cattgaggca cccagctc
//
LOCUS       DAPOZO010000202         3403 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPOZO010000202 DAPOZO010000000
VERSION     DAPOZO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.204"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..76
                     /locus_tag="ROI21_004732"
     CDS             <1..76
                     /locus_tag="ROI21_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDX5113547.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(96..161)
                     /locus_tag="ROI21_004733"
                     /pseudo
     CDS             complement(96..161)
                     /locus_tag="ROI21_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
     gene            394..498
                     /locus_tag="ROI21_004734"
                     /pseudo
     CDS             394..498
                     /locus_tag="ROI21_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            837..1094
                     /locus_tag="ROI21_004735"
     CDS             837..1094
                     /locus_tag="ROI21_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HDX5113548.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(1432..1563)
                     /locus_tag="ROI21_004736"
                     /pseudo
     CDS             complement(1432..1563)
                     /locus_tag="ROI21_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1845..3203
                     /locus_tag="ROI21_004737"
     CDS             1845..3203
                     /locus_tag="ROI21_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HDX5113549.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT          972 a          758 c          806 g          867 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac ctcctcccga actcaaagct atcatacaga
      121 taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac ccagcaatag
      181 tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga caaaacagtt
      241 tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag atctgaagct
      301 tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg aatcaccaca
      361 gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta tatcgagtgc
      421 gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga acaatttgaa
      481 aatcagaatc tcacttaaaa cggcatccat tttacaaaag atggccgtct gtgtatttat
      541 aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata tcctaaaaat
      601 tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga gttgactgaa
      661 tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaac cattagtctg
      721 cttcctgttc ttacaataat cagtattatg tattttattt atatagcaaa atatgcttgt
      781 tacacctgtc ataattaggc atggtaaaat tattgactga accctggggg agaaagatgc
      841 acagtgatta ttataatatg gtttttggtg aaaaattggc caatatttta tatgaagcta
      901 actctcaatt tttttacgag agaaatgtca tagaagaggc agtcaacgct ttattctgtg
      961 agcgtgaaat aataaacaac aagaacataa taaagaagct gatgttcttt ttgtcagatg
     1021 ttaatcacac caagaaagat gttgttcaga gcgcacttaa tattatcatt gacattacat
     1081 caggtgatat ttgagatttt acataaaaga taatctctca aggagtctta tctgaaaacg
     1141 aaatatatag tccctctcgt tttgtaatat cgattggctt tgttgaatcg gttacgtacc
     1201 ccggagccgg tcatattttc aggtaataca cacgctttca gacatttcgg ctttcgtcat
     1261 tttggtatca tgggcatagc ttctgctacc tgaccatcat aatcacgcag cttcagtgaa
     1321 gcagtgacca cctgtttaac ccggtgtatc gttatgatct tatccagcaa taatggacac
     1381 gcggctaagt gaaccgcccc gggtttcctg gagagtgttt tatctgtgaa ctcaggctgc
     1441 cagatcatcg tttccgatgg aagcataata agctttttct gcttctgccg gtgggatatg
     1501 gccctgcctt tccagcaatc gtcgattgtt ataccagtcc acccacgtga gtgtggtcag
     1561 ctcatcatac tgacacgtgc attaccccta ccgcgataat gcaaccgtac aatatagggc
     1621 gtttcctaaa cgcagccctc atcactccaa tccaacaaga cacagcattt ttgatcacga
     1681 agaaagacaa ataatttccg taaatgtttc cgttgttaca aataacaaca gtgttttaca
     1741 tttctttggc ctcaggcccg cgcatgttgc cattcgatgt cattttcctg actcgtttct
     1801 tctttatggt gtagtgtgat cttcacttta tggatgctaa caatatgaaa agaaaaatta
     1861 ttcccgtgct aatcggttgt acgctctctt tctctgccct ggcggcacaa cctacggctg
     1921 agcgctatgt cgtcagtttt ccagaaggca cgcacgtgaa ctacgccggc gcatttgcca
     1981 gtgcattccc gaacggcctt ccggttggga taggttctgg cctgctgttc accggtaaac
     2041 agggcgatgc cctgacattc gcaaccatta ccgatcgcgg tccgaacgcg gattcaccga
     2101 aagaggggaa aaacgaaact aaaattttcg ttacgccaga tttcgcgccg ctgctgatga
     2161 cgattcgtgt acaaaacggc aaagcggagg ccatcgatcc ccgtcctctg catgacgata
     2221 aaggcgcgat caacggtctg ccgctggcaa gcgacgtgat tggctccacc aatgaagtgg
     2281 ccttcagcga cacgttacat agactgaagg gggataatcg cgggctggat acggaaggga
     2341 tcactccgga tggtaaagga ggttactggc tgtgcgatga gtatggcccg ttcctgatta
     2401 acatcgacag taaagggaaa atcctggcaa tccacggtcc gcaggcggcg gaaggggaaa
     2461 aagcgattgc gggcggtttg ccaaatatcc tcaaatggcg tcaggcgaac cgcggctttg
     2521 aaggcctcac ccgtatgccg gacgggcgta ttattgtcgc cgtgcaaagt acgctggata
     2581 tcgacgctaa gagcaagaaa aaggcgctgt ttacgcgtct ggtgagcttc gatccggcaa
     2641 gcggaaaaac cgcgatgtac ggttatccta tcgacagcgc ggcctacagc aaaaacagcg
     2701 acgctaaaat cggagacatc gtggcgctcg ataaccagca catcctgctg atcgaacagg
     2761 gaagagataa aaacaacaga atgcgtaacc tgatttacga ggtcgatctc aataaggcga
     2821 gcgacctgtc aggcttcgac aagccgggtg agtatccgga gtttgatgat gagaaaaccc
     2881 tgtcacagcg cggcattacc cttgcgcaga aaacgcaggt tgttgatctg cgctccttgg
     2941 gctggcagca ggagaaggct gaagggttgg cgctgatcga cagcaaaacg ctggcggtgg
     3001 cgaacgataa cgactttggc gtgaaggtgg cgatgcaaca tccggtcgag ggcaagacat
     3061 tcaaggatta tcgggtgaac gcggaaggaa agctgacgct tgacgacaag caggttgaaa
     3121 cgaccctcag agtgaagcca ctggagaaac cggagtctga cagtgaactg tggattgtga
     3181 cgctgccgga agcgttgaaa taagtttata aggtacggac agcagtatcg cttagttttt
     3241 caaattcatg tatgtgaaca atattggggt tccggcgtag acccccaatg aaagcgtcac
     3301 cacgtgaaaa catgcctgca cggtttacac gtcatctcat ggtggcagcc ggttaacttt
     3361 cctgctacac cagtggaaaa aggccataag cagtccgaac aaa
//
LOCUS       DAPOZO010000203         3203 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPOZO010000203 DAPOZO010000000
VERSION     DAPOZO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.205"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(208..1044)
                     /locus_tag="ROI21_004738"
     CDS             complement(208..1044)
                     /locus_tag="ROI21_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDX5113550.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1044..1847)
                     /locus_tag="ROI21_004739"
                     /note="aph(3'')-Ib"
     CDS             complement(1044..1847)
                     /locus_tag="ROI21_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDX5113551.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(1908..2723)
                     /gene="sul2"
                     /locus_tag="ROI21_004740"
     CDS             complement(1908..2723)
                     /gene="sul2"
                     /locus_tag="ROI21_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDX5113552.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(3031..>3203)
                     /locus_tag="ROI21_004741"
     CDS             complement(3031..>3203)
                     /locus_tag="ROI21_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="HDX5113553.1"
                     /translation="IDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAA
                     GKYDITRPKAAG"
BASE COUNT          733 a          919 c          908 g          643 t
ORIGIN      
        1 tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc ctctctggcg gccttctggc
       61 gctcctgctg cggcgtccgc tcgtgggccg tggcgcgggt cggggtcgtt tgcgggaagg
      121 ggcggaatcc tacgctaagg ctttggccag cgatattctc cggtgagatt gatgtgttcc
      181 caggggatag gagaagtcgc ttgatatcta gtatgacgtc tgtcgcacct gcttgatcgc
      241 ggccgcgata gctagatcgc gttgctcctc ttctccatcc gcgttccaag ctgcggaaag
      301 gcacccataa gcgtacgcct ggtcgagcag gcgacgcgga tcgacgtcca gcgcacgaga
      361 gaatgcgtcc gccatctgtg caatgcgtct aggatcgaga caaaggtcgt ctctgtcagc
      421 cggatcgtag aacatattgg cggcgccaaa gcccacttca ccgaccagac cgacgggatc
      481 tatcaccagc cagccgcgac tggagaacat gatgttttca tgatgcagat cgccatgtag
      541 cccacgcagt tccgaggcat tgctcatcat ttgatcggct ataatcgccg cgtggacgta
      601 gtcagtttga caacctgcgt tttgatcatc gcgcgcccgc tgaaacaaag ctgcaaagcg
      661 atcccggatc gggagaaggg cagaaggcag gggttcctca gatgcggcat acagcttcgc
      721 cattagttcc gctgcaattt cggtcgcctg gtagtcgccg tgctcggcaa cgatgtgaga
      781 gagcattcgc tccccggcat attcgagcaa catcagattg ttctcacgac cgagcaaccg
      841 gactgctccc ctcccattgc gccataccag atagtcggcc ccgcgcagtt catcagcaat
      901 gtcttttata ggtttcaatc ccttgacgat tgcaggagtc ccgtctggca atgaaacttt
      961 ccaaacgagg ctggaaaagg tgtccgcaat gagaacaggt tgcgaaacgt gccaatgagc
     1021 aggaaaaaca ggcggcatga acatcaaccc caagtcagag ggtccaatcg cagatagaag
     1081 gcaaggcgtt cgcggtcggg ggcttcgatc cccaatacat tgaataggac agcgaaggcg
     1141 cgctctgctt catctggcgc tgcccagttc tcttcggcgt tagcaatcat gagtgccaaa
     1201 tcggcatagc gatctgctgt tccgagccgc ccaaggtcga tcagacccgt gcattgaaga
     1261 gttttagggt ccaccatgaa gttcggcatg cagggatcac catggcaaac aaccatatcg
     1321 gtgcgctctt ggtcgagccg caccggtagc tctcgttcga cacgagccaa aagatcgagc
     1381 tgcggcgtac tcttgtcctc gtccggtaag aagtcgggat tgacggcatt gcgggacacc
     1441 acatcaacgg cgcgtccgaa cattcgcgac agcctgcgct caaacggaca ttgatcaacc
     1501 gataggctgt gaacagcgcc aagttgctgc cccattgacg gccacgcttt gagcaaatcc
     1561 gctccagaca gatcagccgc cggtactccc ggaattgccg ttatcaccaa gcatgcaccc
     1621 tcctgttcct cctgccagtt gatgacctcg gggcaagcca cacctcgacc tttgagccaa
     1681 atgaggcggt cacgctctcc agcgagctca ccgcggcggg aagcaggtgc gattttcgcg
     1741 aaggcatgcc cgtcaccacg tcgaaaaaca aaatcaccag attctccgcc tctgacaggc
     1801 aaccagtcag aatgcgattc accaaaaaaa atattagttc gattcaatgg aggttccttc
     1861 agttttctga tgaagcgcga atatagagaa atatcccgaa tgtgcagtta acgaattctt
     1921 gcggtttctt tcagcgccgc caataccgcc agcccgtcgc gcaaggggcg cggctcgtgt
     1981 gtgcggatga agtcagctcc acctgcggcg gcggcaagct ctgcagcgag tgtcgcggcc
     2041 ccgacatccc ccggaccacg gcctgtgagc gcgcgcagaa aggatttgcg cgaaacagac
     2101 agaagcaccg gcaaatcgaa gcgcagccgc aattcatcga accgcgccag caccgagagc
     2161 gaggtttcgg gagcagcccc cagaaaaaac cccatgccgg gatcaaggac aaggcggttg
     2221 cgtttgatac cggcacccgt cagcgccgcg atgcgcgcgt caaagaacgc cgcaatgtga
     2281 tccatgatgt cgccagcggg tgcctcgcgc cgatctgcct gcccgtcttg caccgaatgc
     2341 ataacgacga gtttggcaga tgatttcgcc aattgcggat agaacgcagc gtctggaaaa
     2401 ccgcgaatat cattgagata ggccacacca cgcgacaagg cataggcttg cgtcgcgggt
     2461 tgataactgt cgagcgagac gggaatgcca tctgccttga gcgcgtccag caccggcgcg
     2521 atacgcgcga tttctgtgtc ggacgaaaca ggcgcggcgt cgggattgct ggatgccgga
     2581 ccgaggtcga tcacatctgc cccctcggcc atcagcttac gcgcctgcgc aatggctgcg
     2641 tctggcgcca gataccggcc tccatcggag aaactgtccg aggttatgtt gacgatgccg
     2701 aaaatgatga gcgatttatt catgggggct tctataataa taataatcga gcatgagtat
     2761 catacggatg ctcgggtcga aagggaatcc ccaggcgagt aacctgtttg cggtgatcca
     2821 ttagctgcag gagcagaaga gcatacatct ggaagcaaag ccaggaaagc ggcctatgga
     2881 gctgtgcggc agcgctcagt aggcaatttt tcaaaatatt gttaagcctt ttctgagcat
     2941 ggtatttttc atggtattac caattagcag gaaaataagc cattgaatat aaaagataaa
     3001 aatgtcttgt ttacaataga gtgggggggg tcagcctgcc gccttgggcc gggtgatgtc
     3061 gtacttgccc gccgcgaact cggttaccgt ccagcccagc gcgaccagct ccggcaacgc
     3121 ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca ctggcctctg acggccagac
     3181 atagccgcac aaggtatcta tgg
//
LOCUS       DAPOZO010000204         3189 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPOZO010000204 DAPOZO010000000
VERSION     DAPOZO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.206"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..243)
                     /locus_tag="ROI21_004742"
     CDS             complement(<1..243)
                     /locus_tag="ROI21_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDX5113554.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHL"
     gene            623..>3189
                     /gene="topA"
                     /locus_tag="ROI21_004743"
     CDS             623..>3189
                     /gene="topA"
                     /locus_tag="ROI21_004743"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDX5113555.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     "
BASE COUNT          834 a          761 c          864 g          730 t
ORIGIN      
        1 caggtgatat ttttctgaaa gcacatgggc caggtgtttg aacatattaa acaccgcggt
       61 gcttttggct gttggcaatc cttgttcatc taaaaagtag tcgccggtaa ataccagcac
      121 gccattacgc tgcgtgacgc cggtggcttc aatccctgcg agcgtatcct catgctcacg
      181 aatgattttg ttggcctctt tcaacagcgt ctcgcggtcg atgggttgtg tctctttgtt
      241 cattatttac tccttaaaca aggacattag tctacgccag gcatggcttg cagacaaata
      301 taccacgctg gtggcaagag cgccttactg gcaactttgg attttgcatg ctaataaagt
      361 tgcgtatcgg attttatcag gtacagtgtg acgctttcgt caatctggca atagatttgc
      421 ttgacattcg accaaaattc cgtcgtgcta tagcgcctgt aggccaagac ctgttaactc
      481 agtcacctga attttcgtga acagagtcac gacaaggggt tgatatccgc agagagcgag
      541 tccatatcgg taactcgttg ccagtggaag gtttatcaac gtgcgacgca ttcctggaag
      601 aatcaaatta ggtaaggtga atatgggtaa agctcttgtc atcgttgagt ccccggcaaa
      661 agccaaaacg atcaacaagt atctgggtag tgactacgtg gtgaaatcca gcgtcggtca
      721 catccgcgat ttgccgacca gtggctcagc tgccaaaaag agtgccgact ctacctccac
      781 caagacggct aaaaagccta aaaaggatga acgtggcgct ctcgtcaacc gtatgggggt
      841 tgacccgtgg cacaattggg aggcgcacta tgaagtgttg cctggtaaag agaaggtcgt
      901 ctctgaactg aaacaactgg ctgaaaaagc cgaccacatc tatctcgcaa ccgaccttga
      961 ccgcgaaggg gaagccattg catggcacct gcgggaagtg attggtggtg atgatgcgcg
     1021 ctatagccga gtggtgttta acgaaattac taaaaacgcg atccgccagg catttaacaa
     1081 accgggtgag ctgaatattg atcgtgttaa tgcccagcag gcgcgccgct ttatggaccg
     1141 cgtggtgggg tatatggttt cgccgctgct atggaaaaag atcgctcgtg gtctgtctgc
     1201 cggtcgtgtg cagtcggtgg cggttcgcct ggtggtcgag cgtgagcgtg aaattaaagc
     1261 gttcgtgccg gaagagttct gggaagtcga tgccagcacg accacgccat ctggtgaagc
     1321 gttggcgtta caggtcactc atcagaacga caaaccgttc cgtccggtca acaaagaaca
     1381 aactcaggct gcggtaagtc tgctggaaaa agcgcgctac agcgtgctgg aacgtgaaga
     1441 caaaccgaca accagtaaac ctggcgctcc ttttattacc tctacgctgc aacaagctgc
     1501 cagcacccgt cttggatttg gcgtgaaaaa aaccatgatg atggctcagc gtttgtatga
     1561 agcaggctat atcacttaca tgcgtaccga ctccactaac ctgagtcagg acgcggtaaa
     1621 tatggttcgc ggttatatca gcgataattt tggtaagaaa tatctgccgg aaagtccgaa
     1681 tcagtacgcc agcaaagaaa actcacagga agcgcacgaa gcgattcgtc cttctgacgt
     1741 caatgtgatg gcggaatcgc tgaaggatat ggaagcagat gcgcagaaac tgtaccagtt
     1801 aatctggcgt cagttcgttg cctgccagat gactccagcg aaatatgact ccacgacact
     1861 gaccgttggt gcgggcgatt tccgcctgaa agcacgcggt cgtattttgc gctttgatgg
     1921 ctggacaaaa gtgatgcctg cactgcgtaa aggcgatgaa gatcgtatct taccggcagt
     1981 cgataaaggt gatgctttga cgctcgttga actgacacca gcccagcact ttaccaagcc
     2041 gccagcgcgt ttcagtgaag catcgttggt taaagagctg gaaaaacgcg gcatcggtcg
     2101 tccgtctacc tatgcgtcga tcatttcgac cattcaggat cgtggctatg tgcgagtaga
     2161 aaatcgtcgt ttctacgcgg aaaaaatggg tgaaattgtc accgatcgcc ttgaagaaaa
     2221 tttccgtgag ttaatgaact acgacttcac cgcacagatg gaaaacagcc tcgaccaggt
     2281 ggcaaatcac gaagcagagt ggaaagctgt actggatcac ttcttctcgg atttcaccca
     2341 gcagttagat aaagctgaaa aagatccgga agaggggggg atgcgcccga accagatggt
     2401 tctgaccagc attgactgcc cgacttgtgg tcgcaaaatg gggattcgca cagcgagcac
     2461 cggggtattc cttggctgtt ctggctatgc gctgccgccg aaagagcgtt gcaaaaccac
     2521 cattaacctg gtgccggaaa acgaagtgct gaacgtgctg gaaggcgaag acgctgaaac
     2581 caacgcgctg cgcgcaaaac gtcgttgccc gaaatgcggc acggcgatgg acagctatct
     2641 catcgatccg aaacgtaagt tgcatgtctg tggtaataac ccaacctgcg acggttacga
     2701 gatcgaagag ggcgaattcc gcattaaagg ttatgacggc ccgatcgttg agtgtgaaaa
     2761 atgtggctct gaaatgcacc tgaaaatggg gcgtttcggt aaatacatgg cctgcaccaa
     2821 cgaagagtgt aaaaacacac gtaagatttt acgtaacggc gaagtggcac cgccgaaaga
     2881 agatccggtg ccattacctg agctgccgtg cgaaaaatca gatgcttatt tcgtgctgcg
     2941 tgacggtgct gccggtgtgt tcctggcggc caataccttc ccgaaatcgc gtgaaacacg
     3001 tgcgccactg gtggaagagc tttatcgctt ccgcgaccgt ctgccggaaa aactgcgtta
     3061 tctggccgat gcgccacagc aggatccgga aggtaataag accatggttc gctttagccg
     3121 taaaaccaaa cagcaatatg tctcttcgga aaaagacgga aaggcgactg gctggtcagc
     3181 attttatgt
//
LOCUS       DAPOZO010000205         3165 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPOZO010000205 DAPOZO010000000
VERSION     DAPOZO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.207"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(18..644)
                     /gene="dsbA"
                     /locus_tag="ROI21_004744"
     CDS             complement(18..644)
                     /gene="dsbA"
                     /locus_tag="ROI21_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDX5113556.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(661..1647)
                     /gene="srkA"
                     /locus_tag="ROI21_004745"
     CDS             complement(661..1647)
                     /gene="srkA"
                     /locus_tag="ROI21_004745"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDX5113557.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1724..1993)
                     /locus_tag="ROI21_004746"
     CDS             complement(1724..1993)
                     /locus_tag="ROI21_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDX5113558.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2063..2647
                     /gene="mobA"
                     /locus_tag="ROI21_004747"
     CDS             2063..2647
                     /gene="mobA"
                     /locus_tag="ROI21_004747"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDX5113559.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     AQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2629..3156
                     /gene="mobB"
                     /locus_tag="ROI21_004748"
     CDS             2629..3156
                     /gene="mobB"
                     /locus_tag="ROI21_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDX5113560.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          831 a          767 c          767 g          800 t
ORIGIN      
        1 atgtaattta cattgagtta ttttttctcg gacagatatt tcacagtatc agcatactgc
       61 tgaacaaaaa catccatatt gctggtatcc ataccctgcg gattcagctg atatttaccg
      121 ttaacaaaca tcgccggaac gccacgcaat tgcacgtcag ctgcagcttt ttcctgctga
      181 gcgaccaggg atttcaccac gaagctgttc cacgccgcgt cgtactcttc acctttaata
      241 cctgcgttga taaatacatc gcggatatca gatgctgaac gaatggtctg ggttttctgt
      301 acgccttcaa acagcggaac agtgactttg tcttccacgc ccagtgccat cgccactgcc
      361 catgcctgag tcaaatcttt gcccaggtca ccacccatga agttgacgtg gtatttagtc
      421 atcttcacgc cttccggcag ttttttcttc acgttatcag aaatatgcag aacttcttca
      481 aactgatagc agtgcgggca gaagaaagag aaaaactcca gcacttgcgg cgcgccagct
      541 accggttttt ccagggtagt atactgttta ccatcttcat actgcgccgc cgatgcgcta
      601 aacgctaaaa ctaaaccagc cagcgccagc caaatctttt tcatgatcta ctctctccga
      661 ttaatacata ggtgttaatt gcaaaggggg ttcttgtaga acttttgcct gttctataaa
      721 agtcgccgtc tgtcgcagcc agtaatcttc cccggttaac cacgggaaat ttttcgggaa
      781 cgcgggatca gcccaacgcc gcattagcca ggcaagataa taaaccaaac gcatggcgcg
      841 taaaggttca atcagtccga tttcagcggt gtcgaactcg ctaaattctt cataagcttc
      901 aataatagtt tccagttgca tccgctgctc ggctttatcg ccattgagca acatccacaa
      961 atcctgaacg gctggaccat tacgtgcatc atccagatca acaaacattg gaccatcgcg
     1021 ccagagaata ttcccggcgt ggcagtctcc atgtagccgc agaacggtga aatcttcccg
     1081 ccagtgtgct gtaacggcgg cgatcagctc atctgtcgct ttcaggaatg ccgctttcaa
     1141 cccggaaggt atcagtgtag cgtcctcaaa cagcttgcgt ggctcaatga gatattcatt
     1201 cagaccgatg gtcgggcgat ggataaaaag ctgtttgcgc cccgtctggt gcatacgccc
     1261 taaataacgc ccaaccgctt ccatctgatc gatattatca gcttcgaact ggcgaccacc
     1321 gacgcttgga aaaacagcga aataaaatcc ctgatgattc aataaagtct gaccgttaaa
     1381 ggccacaggc gctgcgaccg gaacttcatc gttaaccagc tgcaacgcaa attgatgttc
     1441 ttcgaggatt tgatcggcag tccaacgttc agggcgataa aatttgacga caaaacgtcg
     1501 acgatcttcg tcctgaaatt gatagacacg gttttcatag ctgttaagcg gggtaagacc
     1561 ggaatccacc cggatcccat gctcaaacag agcgtccatg atggtatccg ggtgtagtgt
     1621 ctggaaagta aaagcgctgt tgttcatccc atcatccgga aaacattacg aatgatgaag
     1681 gatatcattt cacgatgatt ttggtggcgc cgcttacaag cttttactct ttaattacgc
     1741 cgcgagcgcg taacagcgcc gttttgaagt cttcttcata atctttctgc aaacccggga
     1801 tcaccgcatc tttcgcggaa tcacgcattt tcaggtgata gatcagaatg tcatccgtca
     1861 aatccgccag ttcgccgtca aaacctgact ctttcgccag tttctgcaaa aattgcaaca
     1921 ggttaaggtc tggctctttt tgccaggctg gctggaggag ttcaataact tcattcagac
     1981 gtttacattt catagtaatg ctccttactc ttgagacaga cacgttagca gggtcaatcc
     2041 cacaataaaa gaggcgatat cggtgaatct gatgacgacg ataacaggcg ttgtgctggc
     2101 aggcggtaaa gccagacgaa tgggcggcgt agataaagga ttgcttgaat taaacggcaa
     2161 accattatgg caacatgtcg ctgacgcgct tatggcgcag ctctctcacg tcgtggttaa
     2221 tgctaatcgt catcaggaaa tctatcaggc aagcggtctg aaagtgattg aagattcact
     2281 ggcggattac ccaggccctc tggcaggaat gctttcagta atgcagcagg aagcaggcga
     2341 gtggtttttg ttttgtccgt gcgatactcc ttatattccc gaggatttag cagcccggct
     2401 taatcatcag cgcaaagatg cgcctgtcgt gtgggtccat gacggtgaac gcgatcaccc
     2461 gactattgct ctggtaaacc gcgctattga gcctttatta ctggaatatc tgcaagcagg
     2521 agaacgccgg gtaatggcat ttatgcgtct ggctggcggg catgcggttg atttcagcga
     2581 tcataaagat gcatttgtta acgtgaatac gccagaggag cttgcccgat ggcaggaaaa
     2641 acgatgatac cgttactcgc ctttgccgcg tggagtggca ctggaaaaac tacgctgctg
     2701 aaaaaactga tcccggcatt atgcgccaga gggatccgtc cagggctgat taagcatacg
     2761 caccatgata tggatgttga taagccaggc aaagatagct atgagctgcg caaggctggc
     2821 gcggcacaaa ccatcgttgc cagccagcaa cgatgggcct tgatgacgga aacaccagac
     2881 gaagaagagc tggatctaca ttttctcgca agtcgaatgg atacctcaaa gctggatttg
     2941 attctggtcg aagggtttaa gcatgaagag atcgcaaaga ttgtgctgtt tcgcgatgga
     3001 gccggacatc gaccggaaga attagtgata gacaggcatg ttattgctgt agccagtgat
     3061 gtgccgctta atcttgatgt cgcattactg gatattaatg atgttgaggg gctggctgat
     3121 tttgtggtgg agtggatgca aaagcagaac ggataagcaa gagta
//
LOCUS       DAPOZO010000206         3129 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPOZO010000206 DAPOZO010000000
VERSION     DAPOZO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.208"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            629..859
                     /locus_tag="ROI21_004749"
     CDS             629..859
                     /locus_tag="ROI21_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113561.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            911..2272
                     /locus_tag="ROI21_004750"
     CDS             911..2272
                     /locus_tag="ROI21_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDX5113562.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYISDMKITEI
                     PQK"
     gene            2320..2883
                     /locus_tag="ROI21_004751"
     CDS             2320..2883
                     /locus_tag="ROI21_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDX5113563.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(2968..>3129)
                     /locus_tag="ROI21_004752"
     CDS             complement(2968..>3129)
                     /locus_tag="ROI21_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
                     /protein_id="HDX5113564.1"
                     /translation="RKKKNSSSSSSSSSSSSSSSSSSRNNNISNRQMTGMKKQKPPSE
                     RPRNERGGA"
BASE COUNT          718 a          870 c          910 g          631 t
ORIGIN      
        1 cccttgtcgc gccgtggcct gcgctcccgg catcccggca aaaggccgcc gggatgtaag
       61 gggcaacgcc gtttcgcgca cgcgctctcc gtcgttgcgg cgggtccctg ctcattgcct
      121 gccggtgcca cacacccgta tgagtccctg tcgcctgggg ctgatacagc cacaacctgc
      181 cgcactgact gtccagggta aatgaacagc ccacaaaatt ttttacccaa aacccggtaa
      241 aaaattttgc gttctgccca ggacaggtgc gtcaggccgt ggcagtgatg ccccttgcgg
      301 cgaaggttct caaacgggtg aagttcatac cacagggtat acagcagcag gttcccgcga
      361 aacgtcaaaa ccgcggctcc cgaaactcaa cgggtgatgt tctttaaaaa tcaggattta
      421 tttcaggctc cggcaatacg catttatgtg ttctgtcagt catccggctg gcaggtaatg
      481 agaacgcagg aacagaagga ggaaaacacc ggagaacgtc agacgcagat atgaaaaaag
      541 caggtcaggg acctgcttaa tccacaccgc agagactgca cgaacagtct ttacggtttc
      601 attaattaac gtcataagag gctaaattat gacagtaagt attgtttcac cttccgcagc
      661 ggctgtaaag ccccgccgtc atccccgttt cagacgcgaa gacatccccg cgccggaaat
      721 tgaccccgtg ctgaaagcct tcgggcgaca cattgcccgg agttttcacc gggggcgtgg
      781 tgtacacatc cccgccatga aaaacacggc cttcgggcag gtgctccgca cactcgaact
      841 gaaacgcgct tttaactgat gaggtgagcc gccccgcgag gggcggcaac agacggaagg
      901 agaacatttt atgacattag cagcaatcac catgaccgcc ccggaagccg ccagccctgt
      961 gcagatgtac cgcgcgacct actcaccgga tgacaacaaa ctgcgcctgt atgccgtgtc
     1021 acgtcttgac ccggagacgt ataaaaaagt gcatgatgcc ggtttccgct gggcaccaaa
     1081 acaggcgctg tttgtcgcgc cagcctggac accgggccgg gaagacgtgc tcctctcact
     1141 tgccggagag attgaggatg aagacagcac gctcgctgaa cgtcaggaag cacgggcgga
     1201 gcggtttacc ggatacagcg gaaagcgggc cagtgaatcc gcacaggcac ttgatgaagt
     1261 ggaaagactg gccgcgatga tcccgcccgg tcagcccatt cttgtggggc atcacagcga
     1321 acgccgcgcc cgtcgtgatg cgcagcgtat tgaaaacggc atgaaacgag ccgtgatgct
     1381 ctttgaacgt gcggaatact gggaagagcg ggcgcggtcg gcactgcttc acgcgaagta
     1441 taaagaacgt ccggacgttc gctggcgtcg tatcaaaaaa atcgaagctg atttgcgcaa
     1501 ggctgaaaag accatcgcgc agtcgcagaa atatctgacg atgtggcggg ctgaatcgct
     1561 ggatctgaat atggcaaaac tcatcagcag tcatgaccat atcagcgcct gtttcccgct
     1621 ggatacgtat ccgcgcccgg cagaaaaaag ccagtatgaa ggaagtcgat cgttatggtc
     1681 ggccctggat gatgacatca tcaccacgga gcaggcccgc gaaattgcga tccgctgtca
     1741 tgaacggcag attcagcatc aacaacgctg ggttaaccac tatcagaacc gcctgatcta
     1801 tgagcgtgcc atgctggacg aaagcggcgg cgtggttacc cggacacagg attttgagcc
     1861 gggcggacag gttttcagcc ggggcgagtg gctgaccatc atccgtgtga acaaaagcaa
     1921 cggggcggtg agttcagtca caacgccaaa ttacagtttt ctcgggtaca gcggcacgat
     1981 gaaagtgacg cccgatcgca tcacggacta caaagcacca tcggcagaag aggctgccgt
     2041 cgccagtcag gccgcgaagc gtccgccggt agtcaactat ccgggggaag gtttccggga
     2101 aatgacaaag gcacagtggg ccgccctgcc ccgggactgt aaggccgtgc gcagtgtggc
     2161 agaagcagaa gaccacgggg cataccggta ccgccgcaca atggacaata atttccgcct
     2221 ggtgaatgtg tatatctccg acatgaaaat cacggaaatc ccacagaaat aaggtatatc
     2281 cccccgggaa aatccccggg ggatgacgga gataatgata tgcacagtca gttaagagaa
     2341 cgtatccggc tgatgcgcgc aaggctggat aacgccgcgc cggttgctga aatccgggct
     2401 gaatctcagc tttttgtgac tcctgccccg gtctgtgatc gcctggtgat gctggcggag
     2461 atcagcaacc gtgatcacat tctggaaccc tctgccggca ccggggccat actgcgggcg
     2521 attcgggata ccgcgccggg ggccatgtgt gatgcggtgg aaatcaacag cgggctggtc
     2581 cggtatctgc gggaaaattt taacggtgtc agggttcagt gtggcgactt catggaatgg
     2641 ctgccggtgc agtattacag ccgggttatc atgaatccgc cgttcagcca cgggcaggat
     2701 atccggcata tcctgcgggc cttttccctg ttgcgtccgg gtggcgtgct ggtcgccgtc
     2761 tgtctcaacg gaccacgcca gcaggagaag ctgttaccgt tttctgacgt ccgcgaggag
     2821 ctgccgcgcg gcacgtttgc ttataccgat gtcccgacga tgattattcg tctgcgtgcc
     2881 tgatgacaca gggctggcgg gaggataaat gcctgtctgc ccgcgccggg aaaaaacgcg
     2941 cgccggtggc gctgaaaggg gccgcgctca cgcgccgccc ctttcattac gcggacgttc
     3001 tgatgggggc ttctgtttct tcatgcctgt catttgtctg ttactgatgt tgttgttcct
     3061 gctactgcta ctgctactgc tactgctact gctactgcta ctgctactgc tactgttctt
     3121 ctttttcct
//
LOCUS       DAPOZO010000207         3087 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPOZO010000207 DAPOZO010000000
VERSION     DAPOZO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.209"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            222..2192
                     /locus_tag="ROI21_004753"
     CDS             222..2192
                     /locus_tag="ROI21_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDX5113565.1"
                     /translation="MYMNVIRNVICTLIILPIGLQAETSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGIRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDV
                     QFWYQYAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTGNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            2199..2990
                     /locus_tag="ROI21_004754"
     CDS             2199..2990
                     /locus_tag="ROI21_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HDX5113566.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNITAEIGYGHN
                     FPSKGTIPDRRNFFENPRIYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGHELEIIPLS
                     DISRFSEDVKLKVLYKTSPLAGAIMELDSVSYLKSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
BASE COUNT          878 a          702 c          749 g          758 t
ORIGIN      
        1 tggttgtctg gagattcagg gggccagtct aaattccgta ttgctggttc tttatcggta
       61 gaaaaatcaa tatccatagc atcggtcgca ggcgctgata cattgcagag cgaccgggta
      121 ttagagtcgc tattcactgg ttatcactga acgctgcgtg atcagggttg cattaataaa
      181 atgatatgtt ataacatatc aatattaaca tatattttcg catgtatatg aacgtaatca
      241 gaaacgtcat ttgtacatta atcatactcc cgatagggtt acaggcagag accagtcatt
      301 cttctatggc taaagataca atcaccgttg tcgcgacagg aaatcagaac acggtatttg
      361 aaacgccgtc aatggtcagt gtcgtcacga atgacacgcc atggagtcag aatgcggtca
      421 catcagccgg catgctgaaa ggtgttgccg gtctcagcca gactggtgca ggacggacca
      481 atgggcagac ctttaatcta cgcggctatg acaaaagcgg ggtacttgtt cttgttgacg
      541 gcatccgtca actcagtgac atggcaaaaa gcagtggcac ttatctggat ccggcactcg
      601 tcaaacgtat cgaagttgtc cgcggaccaa actccagtct gtacggcagt ggcgggctgg
      661 gaggtgttgt ggacttcaga actgccgatg cagcagattt ccttcccccc ggagagacaa
      721 acggtttaag tctgtgggga aatatcgcca gtggtgacca cagcacaggc tcggggctca
      781 cctggttcgg taaaaccgga aaaacagatg cgctcctttc tgtcattatg cgtaaaagag
      841 gtaatatcta tcaaagtgat ggtgagcgcg cacctaacaa ggaaaaacct gcagccctgt
      901 ttgcgaaagg ctctgtcggt ataacagaca gtaacaaagc aggtgccagc ttgcgtctct
      961 accggaataa caccactgaa ccgggcaatc ccactcagac acatggtgac agcggcctgc
     1021 gtgacagaaa aacagtacaa aatgacgtac agttctggta ccagtacgct cctgtggata
     1081 acagcctcat caatgtaaag tcaactttat atctcagtga tatcactatc aagacaaacg
     1141 gtcacaacaa aacggcagaa tggagaaaca acagaacctc cggtgttaat gttgtcaaca
     1201 ggagtcatac tctgattttt ccgggagccc atcagttaag ttatggcgct gaatattacc
     1261 gtcagcagca gaagccagaa ggctctgcca cactatatcc ggaaggaaac attgacttta
     1321 catcgttgta tttccaggat gaaatgacaa tgaaaagcta cccggttaac attatcgtcg
     1381 gttcccgcta tgaccggtac aagagcttca atccccgtgc cggagaactg aaagccgaac
     1441 gcctgtcccc aagggcggcg atttcagtct caccgacaga ctggctgatg atgtacggct
     1501 ccatatcctc tgcattccgt gcccccacaa tggcagaaat gtacagggat gatgtacatt
     1561 tttaccgcaa gggtaaaccc aattactggg tacctaacct taatctgaaa ccagaaaata
     1621 acattacccg tgagattggc gcaggtattc aactggacgg cctgcttaca ggcaatgatc
     1681 ggctgcagtt gaaaggcgga tatttcggaa cggatgccag aaactatatt gccacgcgcg
     1741 tggatatgaa acggatgcgt tcttattctt ataatgtatc ccgggcccgt atctggggat
     1801 gggatatgca gggaaattac cagtctgatt attttgactg gatgctttct tataaccgca
     1861 cggaaagtat ggatgccagc agcagggaat ggctgggctc cggcaatcct gacacactca
     1921 tcagtgacat aagtatacct gttggtcatg gaggagtttc tgccggatgg cgtgctgaac
     1981 tttcggcagc agccacgcat gtgaaaaaag gcgatcccca tcaggctggc tatgccgtac
     2041 attccttttc actgtcttat aagcctgtaa gtgttaaagg ctttgaggca tcagtgactc
     2101 tggataatgc cttcaacaag cttgccatga atggcaaagg agtgccgctt tcaggcagaa
     2161 ctgtcagtct ttatacccgt tatcagtggt aacaaatcat gatgaaaaat acaggttata
     2221 tcttagctct ttgtctgaca gcatcggggc atgtcctggc ccacgatgtc tggattacag
     2281 gtaaacaggc agagaacaac attaccgcag agattggtta tggtcataat ttcccctcaa
     2341 agggcacaat tcctgacaga aggaatttct ttgaaaatcc ccggatttat aacgggaaag
     2401 agacaataac actgaagcca gcgtccacgg attatgtcta taaaactgag tctgcaagca
     2461 aagataatgg ctacgttttg tcaacatata tgaaaccggg atactggtcg agaacctctt
     2521 caggatggaa accggtcagc cgggagggca gaaatgatgt ggcttactgt gagtttgtca
     2581 ctaaatatgc gaaatctttt attcctggtg aacagcagat gccagcacaa ctctatcagt
     2641 ctccaacggg gcatgagctt gaaatcattc cgttatccga tataagtcgt ttcagtgaag
     2701 atgtgaagct gaaagtcctg tataaaacgt ccccgctcgc cggagctatc atggagcttg
     2761 actcggtcag ttatctgaaa tcatcccgtc atactcatgc agttgagcac aaacatcctg
     2821 ttcataaagc agaactcacc tttgtaacta atgaggatgg tatcgtcaca gtaccttctc
     2881 ttcatattgg acagtggctg gcgaaagtcc aaaataagaa aagttttcag gacaaaagcc
     2941 tgtgtgatga aactgtcgat gtggcaactt taagtttctc ccgaaattaa caatactatt
     3001 ccctgccctg aaattgtaag cgtgcagcaa gaaccgtatt gacggggatg tgttattcag
     3061 tcggcagtgc tacgcgccag gggagca
//
LOCUS       DAPOZO010000208         2974 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPOZO010000208 DAPOZO010000000
VERSION     DAPOZO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.211"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            189..2186
                     /gene="betT"
                     /locus_tag="ROI21_004755"
     CDS             189..2186
                     /gene="betT"
                     /locus_tag="ROI21_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDX5113567.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVSLALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2249..2662
                     /locus_tag="ROI21_004756"
     CDS             2249..2662
                     /locus_tag="ROI21_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HDX5113568.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLLKWSTKTGHRVRVFPVSIFRFVWG"
     gene            complement(2597..>2974)
                     /locus_tag="ROI21_004757"
     CDS             complement(2597..>2974)
                     /locus_tag="ROI21_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDX5113569.1"
                     /translation="TMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQ
                     SMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYN
                     GGLPPNESENRYWKNSNAVASFC"
BASE COUNT          671 a          722 c          746 g          835 t
ORIGIN      
        1 ccttcaggga aaaatatcat cagatggctg cttaaaaaaa gaacaaatgg tagtgtccgc
       61 tattgccagt acacctcaac attccaccat gcattccgat taacgccgca tagccagttg
      121 aactttgcta ctttgtgaga ggtagtacct tctatccagt gcgaatttaa ttaatggaat
      181 aaatgattat gagtgaaaat gatacaatcc caaagaagtc tacaagtcag attaacaaag
      241 cggtattctt tacatctgct ttgctaattt tccttcttgt cgcctttgcc gccgtattcc
      301 cggatgtcgc cgacaaaaat tttaaactac ttcagcaaca aatcttcacg aatgccagct
      361 ggttctacat ccttgctgtg gccctgattt tactgagtgt cacgttcctt ggactctcac
      421 gctacggtga tatcaagctg ggcccggacc atgcgcagcc tgatttcagc taccactcct
      481 ggtttgcgat gcttttttcg gcagggatgg ggatcggcct gatgttcttt ggcgttgccg
      541 aacctgtaat gcattatctt tcgccacccg ttggcactcc agaaaccgtt gcggcagcca
      601 aggaagcaat gcgtctgacc tttttccact ggggactgca cgcatgggca atttatgcca
      661 ttgtggcgct gattctggcg ttcttcagtt accgtcacgg tctgccttta actctgcgct
      721 ccgcactcta tcccattatt ggcgatcgca tatacggacc tgtaggacat gcggttgata
      781 ttttcgctgt tataggcacg gtctttggcg ttgcgacatc actgggttac ggtgttttgc
      841 aggtgaatgc cggtttgaac catcttttcg gggtgcccat caatgaaacg gtgcaggtaa
      901 ttctgatcgt ggtcatcacg gggttagcga cgatttcagt ggtgtccggt ctggataagg
      961 gaatacgtat cctgtctgaa ctcaatctgg gtctggcttt gttgctcctg gcgctggtcc
     1021 tgtgtctggg accaaccgtg cttctgctga agtcatttgt ggaaaatacg ggcggttatc
     1081 tttcggaact ggtgagtaaa acgttcaacc tttacgcgta tgagcccaag tcgagcaact
     1141 ggctgggggg ctggacatta ctgtactggg gatggtggct ttcatggtcg ccgtttgtgg
     1201 ggatgttcat cgcacgggtc tcccgcgggc gaaccattcg cgagtttgtc accggcgtgc
     1261 tgtttgttcc cgcgggtttt acgctaatgt ggatgacggt gtttggtaac agcgcgatct
     1321 atctcattat gaaccagggg gccacagacc tcgccaatac tgttcagcag gatgtgtcgc
     1381 tggccctgtt taatttcctg gagcatttcc cgttctcttc tgtgctgtca ttcattgcaa
     1441 tggcgatggt catcgtcttc tttgtaacgt ctgctgattc gggggcaatg gttgtggata
     1501 ctctggcatc aggtggagtg gcaaacacac ccgtctggca gcgaatattc tgggcctcgc
     1561 tcatgggcat tgttgcaatt gcgcttctcc ttgccggagg gctaagtgcg ctgcaaacgg
     1621 tgacaatagc gagtgcattg cccttctcag tgatcttact aatatccata tacggacttt
     1681 taaaagcttt gcgccgggat ttgaccaagc gtgaaagcct gagcatggcg acaattgctc
     1741 ctacggctgc acgtaaccca attccttggc agagaaggtt acgcaatatc gcgtatctgc
     1801 cgaagcgatc tcttgtgaaa cgttttatgg acgacgttat ccagcccgcc atgacgctgg
     1861 ttcaggagga actgaacaag caggggacga taagccacat tagtgatgca gtcgacgatc
     1921 gtattcgtct tgaagtcgat ttgggcaacg agctgaattt tatatatgaa gtgaggcttc
     1981 gcgggtatat ctcaccgacc ttcgcgctcg ccgcaatgga taatgatgag cagcagactg
     2041 aacaacatcg atattatcgc gctgaggttt atctcaaaga aggcggtcaa aattatgatg
     2101 tgatgggctg gaaccaggaa cagctgatta atgacatact ggaccagtac gaaaaacacc
     2161 tgcacttcct gcacctggtt cgttaatagc aacatgccgt cctgggggcg gcaattatta
     2221 tctcggccgc aatatgaggg aatgcagaat gatttcacgc tggaaatgga tgctgaagca
     2281 gacaattaaa aaactatggt tcagggcaac gttattcgca attgtcgcga taataacggc
     2341 ccttttatca attcttttta aatcaatgat acctgagtcg gtttccgtga aggttggtgc
     2401 ggaagcagtc gataacattc tgaacatact ggcatcgagt atgctggcag tgaccacatt
     2461 ttcgctgagt atcatggtca cagcctacgg ttcagccact actaatgtga ctcccagagc
     2521 tacgcgttta gttgttgaag acgtcaccac acaaaatgta ctggccacct tcatcggttc
     2581 ttttctcttg aagtggtcaa caaaaactgg ccaccgcgtt agagtttttc cagtatcgat
     2641 tttccgattc gtttgggggt aacccaccgt tatattcgtg cggtcttagt gcgctgtaat
     2701 atccaacgat atagtccgtt atggcgtgag ctgcctcgct gaagcttacg taacccacca
     2761 ccggcatcca ttcgttcttc agactcctga agaagcgttc cattgggctg ttatcccagc
     2821 agtttccgcg ccggctcata ctctgtctga tctggtatcg ccacaataac tgccggaact
     2881 gcctgctcgt ataatgactg ccctgatcgc tgtggaacat caccccgccg ggcttaccac
     2941 gggtttccca tgccatttcc agcgctttca tggt
//
LOCUS       DAPOZO010000209         2843 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPOZO010000209 DAPOZO010000000
VERSION     DAPOZO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.212"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..429
                     /locus_tag="ROI21_004758"
     CDS             <1..429
                     /locus_tag="ROI21_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDX5113570.1"
                     /translation="QKQAFGGKVAEHSFGTSTTSPPRFNLLRIEEELSAAHLRLSRTV
                     IEHMDWQQCIERYDRPHTLFYCDPPYLGTEGYGVDFPEGNYSRLAELARCIRGKMIIS
                     VNDIPQMREVFTGLNIQTVNINYSLAGKPTPRRELLICNF"
     gene            complement(546..902)
                     /locus_tag="ROI21_004759"
     CDS             complement(546..902)
                     /locus_tag="ROI21_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDX5113571.1"
                     /translation="MSKDIGERVISVRKKAGLNQREFAARLGISNGGISQIENGKAMP
                     GGEFLLKMHQEFNVDVNWLLTGMSTDGMTRNQNQISPEKQKLIDTFDDMTPDQQRSFL
                     EIGRFYTQPKPGKIAG"
     gene            996..1184
                     /locus_tag="ROI21_004760"
     CDS             996..1184
                     /locus_tag="ROI21_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDX5113572.1"
                     /translation="MTAEQVKALFRQRGITFTRWAEENGYNRNEVYRVLNGQTKARYG
                     KSHEIAVKLGLKSTAQAA"
     gene            1237..1545
                     /locus_tag="ROI21_004761"
     CDS             1237..1545
                     /locus_tag="ROI21_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5113573.1"
                     /translation="MSKTNVSSSGSRILRVLKALRGHALNGISNGELATALHESPANI
                     NRALNTLIEEGLALKLENGRFAPGIQLLQIAMAHSNEMARAQDRINEINQRVISGSRL
                     "
     gene            1556..2476
                     /locus_tag="ROI21_004762"
     CDS             1556..2476
                     /locus_tag="ROI21_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HDX5113574.1"
                     /translation="MGRTKSPINTELNADVPLSDDLNVSLNAMTQHRMEIMQQFGDGL
                     PYERERIVHEARFYMAQSAESMLEAGKRLIILKENEPHGDFTNILENDLGLAPQVARR
                     MMQASVKFLGNGDEQPKRSALSVLGKTKLYELMVLDDEELDALADGGTVAGATLDDID
                     RMTSRELKAALREARETNAAQQQVLAGKDEKINELATKLEKKSRLQPPPPDEELKKLR
                     AEVTALAVEAESAIAVRLSSVFETLCEYCTKNMIDTPRDFMAGLVCQIERSVHALRET
                     FDLEAAPSGNDAPAWLTEPEPEINRPEDMQ"
     gene            2476..2793
                     /locus_tag="ROI21_004763"
     CDS             2476..2793
                     /locus_tag="ROI21_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113575.1"
                     /translation="MNTNNTQNTLMDSPEALGHALCNLLPEMVQGFRVVTPSGEICVP
                     AQETHPFVLTMEVMLMQQIRRLQNQSALRPVVAPQPVNTVVKTCDGETLCDLARKIAA
                     RIG"
BASE COUNT          794 a          721 c          701 g          627 t
ORIGIN      
        1 cagaagcagg cgtttggcgg caaggtggca gagcacagct tcggtacctc caccacatcc
       61 ccgccacgct tcaacctgct acgtattgaa gaagaactgt cagcggcaca cctgcggctt
      121 tccagaacag tcatagaaca catggactgg caacagtgca ttgagcgtta tgatcgtccg
      181 cacacgctgt tctactgcga cccaccgtac ctgggtacgg aaggctatgg tgtggatttt
      241 cctgaaggga actactcgcg gctggcagag ctggcccggt gcatcagagg aaaaatgata
      301 atttcggtga acgacatccc gcagatgcgc gaggtgttca ctggactgaa catacagaca
      361 gtgaatatca actacagtct ggcgggtaaa cccacgccgc gccgtgaact gctaatctgc
      421 aacttctgaa acaaaaccgg aaagcacaat gctttccggt aaaccaaaca acttccacta
      481 tgtcactggc gatgttttct gaaagcatcc agatcaacca cctgtccttt gtgtccgggt
      541 cagtatcagc ctgcgatttt tcctggtttt ggctgagtat agaatcgccc aatctcaaga
      601 aatgagcgtt gttggtctgg tgtcatgtcg tcaaaagtat ctattagttt ttgtttttct
      661 ggggaaattt gattctggtt tctagtcata ccatctgtgc tcattccagt gagcaaccaa
      721 tttacatcaa cattaaactc ttgatgcatc tttaacagaa actcccctcc aggcatcgcc
      781 tttccatttt caatctgact gataccacca ttagaaatac ctaaacgagc tgcaaattct
      841 ctctggttca accctgcttt cttcctcaca gaaataacgc gctctcctat atctttgctc
      901 ataaaagtga atctctatct tgatatgctc ataattatga gcaataatct aacacatata
      961 aggcaaacat cattgcatca acaaaggaga caacgatgac tgcagaacaa gtcaaagctc
     1021 tcttccgcca gcgcgggatc actttcaccc gctgggcaga agaaaacggc tacaaccgca
     1081 atgaggtata ccgcgttctc aacgggcaga ccaaagcccg ttacggtaaa tcccatgaaa
     1141 tcgccgtgaa gttgggactg aaatcaacag ctcaggcggc ctaaatattt tgagtctttg
     1201 taaaaggtta tcacatatcg caaaagaggt atgcagatga gtaagacgaa tgtttcaagc
     1261 tccggcagtc gcatcctccg ggtactcaaa gcgctgcgcg gtcatgccct gaacggtatt
     1321 tctaacggtg aactggcgac agcactgcac gagtcaccgg caaacatcaa ccgggcactc
     1381 aacaccctca ttgaagaagg gctggctctg aaactggaga acggacgttt tgcaccgggc
     1441 atccagttac tgcagatcgc catggcccac agtaacgaga tggcacgtgc acaggatcgt
     1501 attaacgaaa ttaaccaacg tgtcatttca ggtagtcgtt tgtaaggagt aatcaatggg
     1561 acgtaccaaa tcaccgatta acactgaact gaacgccgat gtaccgctgt cggatgatct
     1621 caacgtcagc ctgaatgcca tgacacagca tcgcatggag atcatgcagc agtttggtga
     1681 tggactgcct tatgaacgtg aacgcatcgt tcacgaagca cgtttttata tggcgcagag
     1741 tgctgaatct atgttggaag cgggtaagcg gctgatcatc ttaaaagaaa atgaaccgca
     1801 tggtgatttc acaaatattc tggaaaacga cctcggatta gcaccacagg tagctcgtcg
     1861 catgatgcag gccagcgtga aatttctcgg caatggagat gaacagccaa aacgctcagc
     1921 gctgagcgtt ttgggaaaaa ccaagttata tgaactgatg gttctggatg acgaagaact
     1981 cgacgcatta gctgatggtg gaaccgtcgc cggtgcaacg cttgatgata ttgatcgcat
     2041 gaccagccgc gaactgaagg ccgccctgcg tgaagcgcgc gaaaccaacg cagctcagca
     2101 acaggtgctt gccggaaagg acgaaaaaat caacgaactg gccacaaaac tggagaagaa
     2161 atcgcgcctg caacctccgc cgccagacga agaactgaaa aaattgcggg cggaagtgac
     2221 ggcattagcg gttgaggccg aatcggctat cgccgtccgg ctgtccagtg tttttgagac
     2281 cctgtgtgaa tactgcacca aaaacatgat cgataccccc agagatttca tggccggtct
     2341 ggtctgccag attgaacgca gtgttcatgc attacgtgag acgtttgacc ttgaggccgc
     2401 tccatcgggt aacgatgccc ccgcctggct gaccgaaccg gaacctgaaa tcaaccgacc
     2461 ggaggatatg cagtaatgaa tacgaataac acacaaaata ccctgatgga cagcccggag
     2521 gcactggggc acgcactgtg taatctgctg ccggagatgg tgcagggttt ccgcgtggtg
     2581 accccttccg gtgaaatctg cgtacccgca caggagacgc acccgtttgt actgacgatg
     2641 gaggtgatgc tgatgcagca gataagacgt ctgcagaatc agtcagcatt acgcccggta
     2701 gttgcaccgc aaccggtaaa taccgtggtg aaaacctgcg atggcgaaac actgtgcgac
     2761 ctcgccagaa aaattgccgc cagaatcgga taacagggaa gacaagctat gaccccggca
     2821 ctgactgaaa aactggttga aac
//
LOCUS       DAPOZO010000210         2788 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPOZO010000210 DAPOZO010000000
VERSION     DAPOZO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.213"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            83..352
                     /locus_tag="ROI21_004764"
                     /pseudo
     CDS             83..352
                     /locus_tag="ROI21_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1620..2771
                     /locus_tag="ROI21_004765"
     CDS             1620..2771
                     /locus_tag="ROI21_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113576.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
BASE COUNT          820 a          653 c          682 g          633 t
ORIGIN      
        1 ctctcctgaa tcaggagagc agattacccc ctggatctga tttcaggcgt tgggtgtgga
       61 tcactattgc accgttcgtg acaacaaaag gggtaagctg aaatcgttta tggattacat
      121 cgacatatgc ctggcagaga atgtccggaa tgacgaggtt atctttgcgg agattaaggc
      181 aatggtatac gggccgacgt tccatgccgc gttactacat tcagcccgga cgtaaaatgc
      241 gcccgtcaaa aagaacggtt cgcttcgaaa ctcagccagg ataccacctc tccgccacac
      301 ccgggaagaa gttgatatgg tggttgccgg acaacagcgc tcctggattg actgctgcat
      361 cactcaacca cgctgaatat caaaggagag cgttaccggt taaaagagaa acgtaaagct
      421 gtggtgatga cccaaaacgc aacgcctgtc agtgaggatg aaatggtgaa aagcggacag
      481 catcagtaac ggaagtatct tagcgggtac gaaaatggca ggatcgtgac attgacagaa
      541 tatctcctga tcatcctgaa tgactgcagt tgctgtagaa aagtaagctg tctccaccca
      601 cggcaccact taacaggcat tccgtaactt cccccagact tactatgacg cattatcggt
      661 gcactgaaaa cgggagagtg cgcttaaaac cgcagaccgc atgcgggtgt tactgcatga
      721 cagtctggcc gtattgtgag atgtcggcag gaaccgatgt ccgcgcaggg gggaacttcc
      781 tgcagtacct gacgcttcaa atggtattaa ccgtagggtg ttgttggttc cggatcgcgg
      841 aaatgggagc acaggtctgc tacggggtgt acctctgaca gtatatccgg ctcgctcatc
      901 tgacagcctc tctcccggaa aagaccaaaa atgagatcgt ctcctcaaca gcagagaaga
      961 ggtcttgaca gacaggctac attgcatacg atttatgaag aaaaagacag caaatatgga
     1021 ggttatagcg gagaataccc tacgaaattc ctatgaaaaa cgattgaaaa aaatatcaaa
     1081 ttcgattcgt ttttatatgc tttttgggag cgtggcgatg acgaactgtt gcatgtgcag
     1141 aggagaatgc acccggaagc accagaatca tccagaaagg cagaacgatt acgcatcaaa
     1201 ccaggagaca gcaatatcaa ggtaatatac tgatttaaaa tgaatttaca gcaatacgta
     1261 aaaacatttt ttacttaaag gagacagatg cgctgaagag agtgcaggta tttttatata
     1321 aaaatgagaa gtttacggga tgtatatccg gcacactatg accggagaat atcagtcaag
     1381 cttaaaaaga caataaatac agtgagttaa atacacagag aaaacgtagt atcagagggt
     1441 acatcttaca acagagactg aatttccggt ttttcataac cggcgcattc cggaaagtgc
     1501 aggcggcccg gaggttttct gacgccacta cctggtagat tgttctgtac atcatgtacg
     1561 gaggtttttg tttgcttttt taaaatctgc aaatgtgaga aaagcaggtg aggtgtattg
     1621 tgggaaagga actggatgat atctgcacca gctgtccgta tatcgatgct gtaaaacggc
     1681 acaaacaaca gctcggtgcc atagaggaat atacacagtg gttaaaaaaa gaaccacggg
     1741 catcttattt ttttctgttc cagctgtata cccgcattca caatacattt ttctcccaaa
     1801 agcaacaact cccgttcact cccggtggca ctcactgccc ggaacctgat gtcacccttc
     1861 gggacttaac gctttcccct ggctaccaca gtgattatgc ccctcaaccc attccggaga
     1921 tggacagttc tgctgttgtt cccccaacta atgaaaacac ctcgcctccg gaagatactc
     1981 ccgacaacac tccggccgga ggcaataccg gacaagcgga aaagaccaga aacgcaggac
     2041 tcacacccat tcctgaaaaa cgttcaggaa tgcccccgga acatctgcgg tttgccacgg
     2101 ggtttccatc gcaaccgaag attgccggtc ccagagaaaa gccgatgcgc accgtgcatc
     2161 cggataaaat ctaccgtgaa attatatggt tctgctccag ttacctgctg aaatccggac
     2221 cagaggctac gaggacgatt atcaactcag tattctctga atgggccagc ataaacaatg
     2281 attacccctc ccccttttcg tgggtggaca gcagggacag tgaacagtgt gactggttat
     2341 ggaacgccat gcaggtccgg tgtgtgggaa ccccgctgaa tccccttacc ccggagcaga
     2401 aatactggtt tgcctgcgcc acgtttgata actgggaggg ctggaatgag caacaggtac
     2461 agtttttact ggaaagtaat cccagacgga acagagcgaa gtttacgcag gtctccttcc
     2521 aggcccccag gattcagcat aaagcgattc ttcttgatga gctgaaaagt gcccgggagc
     2581 aacaaaaaag gcgtgatgaa cgtgctgatg gttccgtccc tctgaaactg tccggaaaaa
     2641 tccacaaaca gcttgaaagt attgcccgaa gtcgtggtgt cctcccaaaa aaactgctga
     2701 atgaaatgat tgagcaggcg taccatgatt tggtggcaac caggcagaac agccaaatcg
     2761 attccagata atttcagaca gatattat
//
LOCUS       DAPOZO010000211         2698 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPOZO010000211 DAPOZO010000000
VERSION     DAPOZO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.215"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            1..86
                     /locus_tag="ROI21_004766"
                     /pseudo
     CDS             1..86
                     /locus_tag="ROI21_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(87..725)
                     /locus_tag="ROI21_004767"
                     /pseudo
     CDS             complement(87..725)
                     /locus_tag="ROI21_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(782..1750)
                     /locus_tag="ROI21_004768"
     CDS             complement(782..1750)
                     /locus_tag="ROI21_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="HDX5113577.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNISFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
     gene            complement(1785..2660)
                     /locus_tag="ROI21_004769"
     CDS             complement(1785..2660)
                     /locus_tag="ROI21_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-14"
                     /protein_id="HDX5113578.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGDYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESR
                     RDVLASAARIIAEGL"
BASE COUNT          534 a          783 c          726 g          655 t
ORIGIN      
        1 gagctatgct ggatgaccag gactggcgtg acgccgggtt gcgctatacc tcgcagctcg
       61 atttgctgcc ttttggtcag ttatcccccg cggcggcgcg tcttgcccgc gttctgatag
      121 gacgtacggc cattgtcgct ggcggcaacg acaatttcat ttttcgtgtc cgtattgaag
      181 acgctcaggc tcgccatccc ggtgcccacc tgccatttac tgcctgcttc aacggtggtg
      241 ttagtggccg gttgcagagc aaagttaagc ccggactgac catcgggacg ataggacagc
      301 tcgttaatgg tcggcgtttc aaatcccctc ccggcagaga ggtaaacgtt ccactgcggc
      361 gtcatggcat acttcagcgc tgccgccgga agccacttgt gatagctcgc ttcgccgcta
      421 tcatcaccat ttttacctac aacataacga tcgttggaat caaaccatac cgagctatag
      481 cgcgccccgg cgtccagccc cagggctgag gtgagctgcc agtgcgtttg aatgtaggga
      541 tcgaggttcc acatcaggtt acgttcgtta cgtcgcatat cacctttggt gccgagatcg
      601 taaacgccgt tctgataaac aaagttctca tagcctttgc gctgttcggt catcgcctcg
      661 taatcaaggc cggtggtgat ggcgtaaggg ataaaaccgc tgtcatcgtg gtgtgtccag
      721 cgcgtggctt tgttgaataa atcagatttc gggtaagtct cccccgtagc gggttgtgtt
      781 ttcaggcaat acgcacgctt tcaggcatac ctgctttcgt cattttgttc agcgctcgta
      841 ccagggccat agcctccgca acctgaccat cgtagtcacg cagcgtcagt gaacccccga
      901 acagctgttt tacccggtac atcgccgttt ccgctatcga gcgacggttg taatctgttg
      961 tccatttcca ccgcgcatta ctcccggtca ttcgctgatt agccactgca cggttacggt
     1021 ctgcatattc accgggccag taacccgcac cttttcgggg cgggataagc gcgctgattt
     1081 tcttacgccg cagttcatcg tgacagagcc gggtatcgta agctccatcg gcggcggctg
     1141 acctgatttt ccggtgggtt tgccggatta acccggggaa ggcctctgag tccgtaacgt
     1201 tgttcagcga caggtcagcg cagatgattt catgtgtttt actgtcaact gccagatgca
     1261 gcttacgcca tatacgacgg cgttcctggc catgcttttt gactttccac tcgccttcac
     1321 cgaagacctt cagcccggtg gaatcaatta ccaggtgtgc gatttcaccc cgggtgggcg
     1381 ttttgaaact gatattaacc gactttgccc gcctgctgac acagctgtaa tccgggcagc
     1441 gtagcggaac gttcatcaga gaaaaaatgg aatcaataaa gccctgcgca gcgcgcaggg
     1501 tcagcctgaa tacgcgttta atgaccagca cagtcgtgat ggcaaggtca gaatagcgct
     1561 gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaata gcttcatcat
     1621 ccagccagaa agttatggag ccacggttga tgagggcttt attgtaggtg ggccagttgg
     1681 tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tacgtgatct
     1741 gatccttcaa ctcagcaaaa gttcgattta ttcaacaaaa ccagttacag cccttcggcg
     1801 atgattctcg ccgctgaagc cagcacatcg cggcggctct ctgcgttctg ttgcggctgg
     1861 gtaaaatagg tcaccagaac cagcggcgca cgaccctgcg gccagatcac cgcaatatca
     1921 ttggtggtgc cgtagtcgcc gctgccggtc ttatcaccca cagtccacga cgtcggtaag
     1981 ccggcccgaa tgctggctgc gccggtcgta ttgcctttga gccacgtcac caactgcgcc
     2041 cgctgggttt cgcccagcgc atgacccagc gtaagctgac gcaacgtctg cgccatcgcc
     2101 cgcggcgtgg tggtgtctct cgggtcgccg ggaatggcgg tattcagcgt aggttcagtg
     2161 cgatccagac gaaacgtctc atcgccgatc gcgcgggcaa aagccgtcac gcctcccggg
     2221 ccaccgagct gggcaatcaa tttgttcatg gcggtattgt cgctgtactg caacgcggcc
     2281 gcgctcagtt ctgccagcgt cattgtgccg ttgacgtgtt tttcggcaat cggattgtag
     2341 ttaaccagat cggcaggctt gatctcgaca ggctgattaa gcagctgctt ttgcgtttca
     2401 ctctgcttaa gcaccgccgc ggccgccata actttactgg tactgcacat tggaaagcgt
     2461 tcatcaccgc gataaagcac ctgcgtatta tctgcggtat cgatgagcgc gacgcccagc
     2521 cgccctccgc tgcttttctc cagcgccgcc agcttttgct gcaccgcact cgtctgcgca
     2581 taaagcggcg cgctgcccag cagcagcgga atgcacgccg ccgccgcgaa catcatccgt
     2641 tgcactctct ttgtcaccat ctcaaactcc caatacggtc aatccgtgtt acatcagt
//
LOCUS       DAPOZO010000212         2576 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPOZO010000212 DAPOZO010000000
VERSION     DAPOZO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.216"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..542
                     /gene="tolA"
                     /locus_tag="ROI21_004770"
     CDS             <1..542
                     /gene="tolA"
                     /locus_tag="ROI21_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDX5113579.1"
                     /translation="AAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAA
                     AAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQI
                     KSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPK
                     PPSQAVYEVFKNAPLDFKP"
     gene            675..1967
                     /gene="tolB"
                     /locus_tag="ROI21_004771"
     CDS             675..1967
                     /gene="tolB"
                     /locus_tag="ROI21_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDX5113580.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            2002..2523
                     /gene="pal"
                     /locus_tag="ROI21_004772"
     CDS             2002..2523
                     /gene="pal"
                     /locus_tag="ROI21_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDX5113581.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
BASE COUNT          663 a          623 c          696 g          594 t
ORIGIN      
        1 aagctgcagc cgacaaaaaa gcagcagaaa aagcggctgc tgaaaaggca gcagctgata
       61 agaaagcagc ggcagaaaaa gccgccgcag acaaaaaagc ggcagctgca aaagcagcag
      121 ctgaaaaagc cgctgcagca aaagctgccg cagaggcaga tgatattttc ggtgagctaa
      181 gctctggtaa gaatgcaccg aaaacggggg gaggggcgaa agggaacaat gcttcgccag
      241 ccgggagtgg taatactaaa aacaatggcg catcaggggc cgatatcaat aactatgccg
      301 ggcagattaa atctgctatc gaaagtaagt tctatgacgc atcgtcctat gcaggcaaaa
      361 cctgtacgct gcgcataaaa ctggcacccg atggtatgtt actggatatc aaacctgaag
      421 gtggcgatcc cgcactttgt caggctgcgt tggcagcagc taaacttgcg aagatcccga
      481 aaccaccaag ccaggcagta tatgaagtgt tcaaaaacgc gccattggac ttcaaaccgt
      541 aatcgcgatg ttgactgttc cgacggtcaa catcaggcac cggttgccac ggggttctgg
      601 tagttttgtg tattttagtt tgttaacatt ctgctaaatt atcgtgggcc atcggtccag
      661 ataagggaga tatgatgaag caggcattac gagtagcatt tggttttctc atactgtggg
      721 catcagttct gcatgctgaa gtccgcattg tgatcgacag cggtgtagat tccggtcgtc
      781 ctattggagt tgttcctttc cagtgggcgg gtcctggtgc ggcacctgaa gatattggcg
      841 gcatcgttgc tgctgacttg cgtaacagcg gtaaatttaa tccgttagat cgcgctcgtc
      901 tgccacagca gccgggtagt gcgcaggaag tacaaccagc tgcatggtcc gcactgggca
      961 ttgacgctgt agttgtcggt caggtcactc cgaatccgga tggttcttac aatgttgctt
     1021 atcaacttgt tgacactggc ggcgcaccgg gtactgtact tgctcagaac tcgtacaaag
     1081 tgaacaagca gtggctgcgt tatgctggtc ataccgccag tgatgaagtg tttgaaaaac
     1141 tgaccggcat taaaggtgcg ttccgtaccc gtattgccta cgttgttcag accaacggcg
     1201 gtcagttccc gtatgaactg cgcgtatctg actatgacgg ttacaaccag tttgtcgttc
     1261 accgttcacc gcagccgctg atgtctccgg cgtggtcacc agacggttct aaactggctt
     1321 atgtgacctt cgaaagcggt cgttccgcgc tggtcatcca aacgctggca aatggcgctg
     1381 tacgtcaggt ggcttcattc ccgcgtcaca acggtgcacc tgcattctcg ccagatggca
     1441 gcaaactggc attcgccttg tcgaaaaccg gtagtctgaa cctgtacgta atggatttgg
     1501 cttctggtca gatccgccag gtgacggatg gtcgcagtaa caataccgaa ccgacctggt
     1561 tcccggacag ccagaacctg gcatttactt ctgaccaggc cggtcgtcca caggtttata
     1621 aagtgaatat caacggcggt gcgccacaac gtattacctg ggaaggttcg cagaaccagg
     1681 atgcggatgt cagcagcgac ggtaaattta tggtaatggt cagctccaat ggtgggcagc
     1741 agcacattgc caaacaagat ctggcaacgg gaggcgtaca agttctgtcg tccacgttcc
     1801 tggatgaaac gccaagtctg gcacctaacg gcactatggt aatctacagc tcttctcagg
     1861 ggatgggatc cgtgctgaat ttggtttcta cagatgggcg tttcaaagcg cgtcttccgg
     1921 caactgatgg acaggtcaaa ttccctgcct ggtcgccgta tctgtgataa taattaattg
     1981 aatagtaaag gaatcattga aatgcaactg aacaaagtgc tgaaagggct gatgattgct
     2041 ctgcctgtta tggcaattgc ggcatgttct tccaacaaga acgccagcaa tgacggcagc
     2101 gaaggcatgc tgggtgccgg cactggtatg gatgcgaacg gcggcaatgg caacatgtct
     2161 tccgaagagc aggctcgtct gcaaatgcaa cagctgcagc agaacaacat cgtttacttc
     2221 gatctggaca agtacgatat ccgttctgac ttcgctcaaa tgctggatgc acacgcaaac
     2281 ttcctgcgta gcaacccgtc ttacaaagtc accgtagaag gtcacgcgga cgaacgtggt
     2341 actccggaat acaacatctc cctgggtgaa cgtcgtgcga acgccgttaa gatgtacctg
     2401 cagggtaaag gcgtttctgc agaccagatc tccatcgttt cttacggtaa agaaaaacct
     2461 gcagtactgg gtcatgacga agcggcatac tccaaaaacc gtcgtgcggt actggtttac
     2521 taagagaatt gcatgagcag taacttcaga catcaactat tgagtctgtc gttact
//
LOCUS       DAPOZO010000213         2529 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPOZO010000213 DAPOZO010000000
VERSION     DAPOZO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.218"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..1158
                     /locus_tag="ROI21_004773"
     CDS             <1..1158
                     /locus_tag="ROI21_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5113582.1"
                     /translation="ASKATYINKSGVLTEAAIDEPRFERDGLLIEGQRTNYMLNSESP
                     ASWGRSSNMDVPETGTDNFGFTYGKFVCNDSLIGQTSAINMASIAATKSVDVSGDNKH
                     VTTSCRFKTELQVRLRIRFDKYDGSATTFLGDAYIDTQTLEINMTGGAASRITARVRK
                     DEATGWIFAEATIQAIDGELKIGSQIQYSPKQSGATVSGDYIYLATPQVEDGPCVSSF
                     IISGATAATRASDIVTVPIKNNLYNLPFTVLCEVHKNWYKTPNAAPRVFDTGGHQTGA
                     AIILGFGRSTDYDGFPYCDIGGANRRVNENASLEKMVMGMRVKSEQSTCSVSNGHISS
                     ETKTTWSCIQNTAIIRIGGQTTAGLRHLFGHVRNFRIWHKALTDAQVGESI"
     gene            1158..1439
                     /locus_tag="ROI21_004774"
     CDS             1158..1439
                     /locus_tag="ROI21_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113583.1"
                     /translation="MKDLTLKFADRADFSAFMESIGYYDDESMQDDILIDVIGNVYKE
                     TGELTEDGEPVCVKEDGYFVNVRIINDSQISSLFDEYVVAVEHQLRGWM"
     gene            1449..2489
                     /locus_tag="ROI21_004775"
     CDS             1449..2489
                     /locus_tag="ROI21_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HDX5113584.1"
                     /translation="MATSTVIPDDIKTLKSDVSKLKNDQGSYATKLYVDSKDEIVGDW
                     SASWYQQVLPTSGAIFGRKLRSTHRTAGVEDAYCELYLKKWIDSPGNAMARLNLNDNG
                     TNICWDFTNLYGGTMIFPGDSGYLKMGNCLMSYSKRGSNALIKFDYTDTLQIKYANHG
                     STMTLNTQGTAYAGVTAQLWGNSSRPVVYEVGVDGGAYMFYAQKNTDNTYMLSVNGAC
                     HATAFNQHSDRDLKDNIQVIDNATDRIRKMNGYTYTLKENGMPYAGVIAQEALEAIPE
                     VVGSAMKYQDGASGSEGEEGERYYTVDYSGVTGLLVQVARESDDRITALEEENAELKQ
                     RLSAIEATLASK"
BASE COUNT          768 a          498 c          622 g          641 t
ORIGIN      
        1 gcatcgaaag caacctatat taacaaatct ggtgtgctga cagaggctgc cattgacgag
       61 ccacgatttg aacgtgatgg cctgcttatt gaggggcaaa gaacaaacta catgctcaat
      121 tcggaaagcc ctgccagttg ggggcgatcg tcaaatatgg atgtgcccga aacagggacg
      181 gataattttg gttttaccta tggaaagttt gtctgcaacg attctctgat tgggcaaacc
      241 tcagccatta atatggcatc aattgctgca acaaagtcag ttgatgtctc aggcgataat
      301 aaacacgtga caacctcatg tcgttttaaa acagaactgc aggtaaggtt gcgtatccgg
      361 tttgataaat atgacggtag cgcaacaact tttcttggtg atgcgtatat tgatacacaa
      421 acgcttgaaa ttaatatgac aggcggtgct gcctcaagga ttacagcgag agtcagaaag
      481 gacgaagcta ccggatggat ttttgcagag gcaacaattc aggcaattga tggggagtta
      541 aaaataggct ctcagataca gtattctcct aagcagagcg gggcaaccgt atctggtgac
      601 tatatttatc tggccacccc acaagtagaa gatgggcctt gtgtatcatc ttttattata
      661 tcaggagcga cggcggcgac ccgcgcaagc gatatagtta cagttccaat taagaataat
      721 ctttataatc ttccttttac ggttctttgt gaggtacata agaactggta taaaacgcca
      781 aatgcagcgc cacgtgtttt tgataccggc ggtcatcaaa ccggagcggc tattattctt
      841 ggcttcggtc gttcaacaga ttacgacgga tttccttatt gcgatattgg aggagctaac
      901 agacgggtaa acgaaaacgc atcgcttgaa aaaatggtta tggggatgcg tgtaaagtca
      961 gagcagtcta cgtgctcagt aagtaacggg catatatcca gcgaaacaaa aaccacatgg
     1021 tcctgtattc agaacaccgc aattatccgt attggaggcc agactacagc cgggttacgt
     1081 catttatttg gtcatgtcag gaatttcaga atatggcaca aggcattgac tgatgctcag
     1141 gtgggggagt caatctaatg aaagatttaa cactcaaatt tgccgacagg gccgactttt
     1201 cggcctttat ggagagtatt ggctattatg atgacgagtc gatgcaggat gatattctta
     1261 ttgacgtgat aggtaacgtg tacaaagaaa ccggagaact gactgaagat ggcgaaccgg
     1321 tatgtgttaa ggaagacgga tattttgtaa acgtgcgcat cattaatgat tcgcaaatat
     1381 cgtcattatt cgatgaatac gtggttgctg ttgagcatca acttcgtggc tggatgtgag
     1441 gaagaaaaat ggctacatcg acagtaattc ctgatgacat caaaacgcta aaatccgacg
     1501 ttagcaaatt aaaaaacgat caaggaagct acgcaacaaa attatatgta gacagcaaag
     1561 atgaaatcgt tggtgactgg tctgcttcat ggtatcagca ggtattgcca actagcggag
     1621 ctatatttgg gagaaaactc cgctcaactc acaggacggc aggtgttgag gatgcgtatt
     1681 gcgaactata cctcaaaaaa tggatagaca gtccaggtaa cgcaatggcg cgccttaacc
     1741 tgaacgataa cgggacaaac atttgctggg actttaccaa cctttatggc ggtacgatga
     1801 tttttcccgg tgacagcgga tacctcaaaa tgggtaactg ccttatgtca tacagcaagc
     1861 gtggaagtaa cgcgcttatt aaatttgatt acaccgacac attacagatc aaatatgcca
     1921 atcatgggtc aaccatgaca ttaaacacac agggaaccgc ttatgctggt gttactgctc
     1981 aattgtgggg caactccagc cgtcctgttg tttatgaagt cggtgttgat ggtggcgctt
     2041 atatgttcta tgcgcagaaa aataccgata acacctatat gttaagcgtt aatggtgcat
     2101 gtcatgccac cgcatttaac cagcattccg accgggatct gaaagacaac attcaggtga
     2161 tcgataatgc aaccgaccgc atccgtaaaa tgaacggcta tacatacacg cttaaagaaa
     2221 acggtatgcc ctatgctggt gtcattgcac aggaagctct ggaagcaatc ccagaagttg
     2281 taggttccgc aatgaaatat caggacggtg caagcggatc ggaaggtgaa gaaggtgaac
     2341 gttattacac agtagattat tctggtgtta ctggcttgct tgttcaggta gccagagagt
     2401 cagacgacag aataacagca ctggaagaag aaaatgcaga attaaaacaa agattatctg
     2461 caattgaggc gacgcttgcg tctaaataat attaaggggc cgagcgcccc gttttattgg
     2521 gtaggatga
//
LOCUS       DAPOZO010000214         2482 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPOZO010000214 DAPOZO010000000
VERSION     DAPOZO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.219"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..207
                     /locus_tag="ROI21_004776"
     CDS             <1..207
                     /locus_tag="ROI21_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDX5113585.1"
                     /translation="RGEAMRIHQQHPTSRLFPFCTGKYRWHGSTEAYTGREVQDIPGV
                     LAVFAERRKDSFGPYVRLMSVTLN"
     gene            226..870
                     /locus_tag="ROI21_004777"
     CDS             226..870
                     /locus_tag="ROI21_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HDX5113586.1"
                     /translation="MSSIITITVYTLDELSCPARGKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQSATARRIR
                     EHAPQERELHRIADTLQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQAMT
                     TDAEAAVSEALRDLAFWLYRQLENKYDWLTSDTAVDEALLINEYTFTEAGLRAG"
     gene            920..1300
                     /locus_tag="ROI21_004778"
     CDS             920..1300
                     /locus_tag="ROI21_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDX5113587.1"
                     /translation="MSDTLPGTTLPDDNKDLPWWGLPCAVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCDAD
                     TLGSCGYVYLAVYPATETESNPPE"
     gene            1377..1754
                     /locus_tag="ROI21_004779"
     CDS             1377..1754
                     /locus_tag="ROI21_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDX5113588.1"
                     /translation="MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKHPEAKR"
     gene            1751..2239
                     /locus_tag="ROI21_004780"
     CDS             1751..2239
                     /locus_tag="ROI21_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113589.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVELAELINVVCDNGYS
                     LRVVDESDRTSTDSTPPSAALSGIRCSTAHITETDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            2256..2453
                     /locus_tag="ROI21_004781"
     CDS             2256..2453
                     /locus_tag="ROI21_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDX5113590.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IKQAREDIRTLRQQQLLQQNR"
BASE COUNT          579 a          696 c          650 g          557 t
ORIGIN      
        1 cgtggtgaag ccatgcgtat tcaccaacaa catccgacat cccgtctttt cccgttctgt
       61 accggcaaat accgctggca cggcagcact gaagcgtata ccggtcgtga agtgcaggat
      121 attcccggtg tgctggccgt gtttgctgaa cgtcgtaagg acagttttgg cccgtatgtc
      181 cggctgatga gcgtcactct gaactgagtc agtaaaggca ttctgatgag cagcattatc
      241 accatcacag tgtatacgct ggatgaactt tcctgcccgg cacggggaaa agcccgcgac
      301 tggtaccgtc agcatcatgc tgacagcaac tggtatgaaa acgtgtatga agacttcagg
      361 gaggtctgtg gcattttcgg gattgacctc cgccagcgcg ttttccggct cagcaatggc
      421 aggtttatgg aagagccgtg catctggttc agcggattct gcagccaggg ggacggagcc
      481 tgttttgaag gacgctggca ctggcagtct gcaacagccc ggagaatcag ggagcatgca
      541 ccgcaggagc gtgagcttca tcgtattgct gataccctgc aggcggtgca gaaacgtaac
      601 ttctggcagc ttcaggctga aatcagtcac cggggacgtt actgccatcc gtacagcatg
      661 gatatcactg ttacccggaa cagtccgact ggtcaggcaa tgacaaccga tgcggaagcc
      721 gctgtcagcg aagccctgcg cgacctggcc ttctggctgt accgccagct ggaaaacaag
      781 tacgactggc tgacctcaga caccgctgtg gatgaggccc ttctcatcaa cgaatatacc
      841 ttcaccgaag ccggtctgcg cgccggctga cccgtcatcg ttaaactatc attcaccgat
      901 tttctgtgag gattttatca tgtcggacac actccccgga acaacgcttc ccgacgacaa
      961 caaagacctc ccctggtggg ggctgccctg cgccgtgaca ccttgttttg gcgcccgtct
     1021 ggtgcaggag ggtaaccggt tgcattacct tgccgaccgc gccggtatca ggggccggtt
     1081 cagcaatgcg gatgcatacc atctggacca ggcttttcca ctgctgatga aacaactgga
     1141 actcatgctc accagcggtg aactgaatcc ccgccatcag cataccgtca cgctgtatgc
     1201 gaaagggctg acctgcgatg ccgacaccct cgggtcctgt ggttacgttt atctggctgt
     1261 ttatcctgcg accgaaacgg aaagtaaccc tccggaatag tcttctgctc cggcctggtg
     1321 ctttctccac gccacttttc catttcttat atctgcatat caggaaaatc ttcagtatga
     1381 aaacattacc cgacacacac gtacgggcgg catcgcgctg cccgtctccc gtcaccatct
     1441 ggcagacact gctcacccaa ctgctggacc agcactatgg cctcacgctg aatgacacac
     1501 cgttcgctga tgaacgtgtg attgagcagc atattgaggc agggatttca ctgtgtgacg
     1561 cggtgaactt tctcgttgaa aaatacgcgc tggtacgtac cgaccagccg ggattcagcg
     1621 caggagcccc gtcgcagtta atcaacagca ttgatattct ccgggctcgc agggcgaccg
     1681 gcctgatgac ccggaacaat tacagaatgg taaataacat tacccagggc aagcatccgg
     1741 aggcaaaacg atgaaactgg ccctgacgct ggaagctgac agcgttaacg tacaggcact
     1801 gaacatgggg cgaattgtcg ttgacgtcga tggtgttgag cttgccgaac tgattaacgt
     1861 ggtctgcgat aatggctact cccttcgtgt tgttgatgaa tctgaccgga cctcaacaga
     1921 cagcaccccc ccctctgctg ccctgagcgg tatacgctgt agtaccgcgc atatcacgga
     1981 aacagacaac gcctggctgt actcgctgtc gcatcagacg aatgacaccg gcgaatcaga
     2041 atggattcat tttacaggta gcggatatct gttacgtacc gatgcgtggt catacccggt
     2101 tctgcggctt aaacgcctgg ggctgtcaaa aacgttccgc cgtctggttg tcacacttac
     2161 ccgacgttat ggcgtcagtc tcattcatct ggatgccagc gctgaatgcc tgccggattt
     2221 acccactttc gactggtaac ccggaaattc acaacatgaa atcattaacc acggaaacag
     2281 cgctggatat tctgattgcg tggctgcagg acaatattga ctgcgaatca gggattatct
     2341 tcgacaacga tgaggataaa acggattcag cagcactgct gccctgcatt aaacaggcca
     2401 gggaagatat ccgtaccctg cgccaacagc agcttctgca acagaacagg tgagtcttac
     2461 tcaccagact ttattccact ga
//
LOCUS       DAPOZO010000215         2419 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPOZO010000215 DAPOZO010000000
VERSION     DAPOZO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.220"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            144..1409
                     /locus_tag="ROI21_004782"
     CDS             144..1409
                     /locus_tag="ROI21_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDX5113591.1"
                     /translation="MALTDAKIRAAKHTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRNITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTSVKRQHRPALDLSRLPELLSRINS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAERKPLPGVKFSHRGS
                     KMRTPHLVPLSQQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLIL
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            1665..>2419
                     /locus_tag="ROI21_004783"
     CDS             1665..>2419
                     /locus_tag="ROI21_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDX5113592.1"
                     /translation="MTGKLRFAVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAYLAYVFLEQNAPRKALNAALKGLAVGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLQRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWG"
BASE COUNT          663 a          600 c          549 g          607 t
ORIGIN      
        1 atttaagcgt cctgccgcca aatcaattct accgaactca accagactcc ccccacatca
       61 accccattgt gtgggtataa ttgcgggtat accccagttc gacagaattt gtaccctctt
      121 taacgcattc agaaggctga actatggcac tgactgacgc aaaaatccgg gctgcaaagc
      181 acactgacaa ggcttataaa ctcactgacg gggctggcat gttcctgctg gtacatccta
      241 atggttcccg ttactggcgt ctccgttatc gcattctggg taaggagaaa actctggcac
      301 ttggtgtata cccggaggtt tctctctccg aagctcgtac aaaacgggat gaggcccgaa
      361 aactgatttc ggaggggatt gacccttgcg aacagaaaag agctaaaaaa gtagtccctg
      421 atttacaact ctcttttgaa catattgcac gacgctggca tgccagtaat aaacaatggg
      481 cacaatcaca cagcgataag gtactcaaaa gcctcgaaac acacgttttc ccctttatcg
      541 gcaaccggaa catcacaaca ctcaataccc cggacctgct tatccctgtt cgtgctgcag
      601 aagctaaaca aatttatgaa atcgccagtc gtctgcagca aagaatatct gctgtaatgc
      661 gttatgccgt acagtctggc atcatcagat ataatcctgc tctggatatg gctggcgcat
      721 tgacctctgt aaaacgacag catcgccccg ctcttgatct ttcacgtctg cctgaacttc
      781 tgtcgcgtat taacagttat aaaggtcagc ctgtcacccg gcttgctgtt atgctgaatt
      841 tactggtttt tattcgctcc agtgaactca gatatgcccg ctggtctgaa attgatattg
      901 acaatgccat gtggactatt ccagccgaac gcaaacctct gccaggcgta aaattctcac
      961 accggggctc caagatgcga acaccacatc ttgtgccact cagccaacag gctgtagcca
     1021 tactggcaga acttcagaca tgggcaggtg aaaatggtct gatctttacg ggagcacacg
     1081 acccacgtaa accaatcagt gaaaataccg taaataaggc cctcagagtg atggggtatg
     1141 acacaaccaa ggaagtctgt ggccatggat tccgggcgat ggcgtgcagt gcattgattg
     1201 aatccggctt atggtcaaga gatgcggttg aacgtcagat gagtcatcag gagcgcaacg
     1261 gagtacgtgc cgcgtatatt cataaagcag aacatctgga agaacgtcgg cttatactgc
     1321 aatggtgggc agattttctg gatgcaaaca gggaggagtg tatcagtccg tttgaatatg
     1381 caaaagttaa caatccacta aaaaggtgac tgaacgccat ttacacacat gctcttcctc
     1441 ttaaggggaa gagcacccgc ctcaatcaat ttctttttta tgccggcgga accatcagcc
     1501 aggctgaggt tctcattcaa taactcaacg tcttaccaca gaacagaatt cactgtatta
     1561 tcatgggcaa cgttaatccc ggacaataaa aggacaccga tgccaggcat cacaattttt
     1621 atagcctgca ggttatggtc catccttttt tacaggttca gaatatgact ggcaaattac
     1681 gctttgcagt gaacgataac cagggctgtt tcatatttcc ggaaacctgg tttggctcgt
     1741 tgctggatga gtttgaagaa ctgattgatg catacgatgc ggatgaaatc agcgaaacaa
     1801 gttacatcaa caaactcagg cgccttgccc gacaggagaa tgactttatt gatgtacacg
     1861 cttacctggc atacgtattt ctggagcaga atgccccgcg taaggcgctg aatgcagccc
     1921 ttaaagggct ggcagttggc aatcgtctta tccctgaagg tttcagtggc agaatcatct
     1981 ggatacatcc tgacaaccga ccttttcttc gtgccctcta tgcggcaatc ctggcaaatg
     2041 cacatttaca gcgtcatcag gatgccatca tgctcattga aaaaatactg gattacaatc
     2101 ccgaagataa tcatggtgca cgctggctac tgggaccgga actgttacgc actggcgctc
     2161 atgaacaggc acgccatata ctgcaggaac atgcagatga attttcacct tactggtatg
     2221 aactcggcct tctgcatttt cttaatgggg aactggtaaa agctgcaact gcatttcgtc
     2281 gcggctttgc ggcaaatact tacatcgcag aaatactgtg cggaaatctg catccatttc
     2341 cacttgctgt atggcataac ttttcaggcg gtccggatac tgccgaagat tattatgcca
     2401 cataccaccc gttatgggg
//
LOCUS       DAPOZO010000216         2362 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPOZO010000216 DAPOZO010000000
VERSION     DAPOZO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.221"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            9..854
                     /locus_tag="ROI21_004784"
     CDS             9..854
                     /locus_tag="ROI21_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDX5113593.1"
                     /translation="MSDITASRPEVVNGHTEVICSTSIHHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTTRDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     RMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFKQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIINNLVRWDRWRFHLITGQQTDRLADLERMLHLFSDKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPN
                     VLADR"
     gene            923..1318
                     /locus_tag="ROI21_004785"
     CDS             923..1318
                     /locus_tag="ROI21_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDX5113594.1"
                     /translation="MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITEALERLNIRYRLAGATECYNKGQ
                     STQIPVSLGIKTPSRFKRVLIVGKSKINA"
     gene            1311..2245
                     /locus_tag="ROI21_004786"
                     /pseudo
     CDS             1311..2245
                     /locus_tag="ROI21_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
BASE COUNT          736 a          516 c          526 g          584 t
ORIGIN      
        1 tatcgattat gtctgatatc acagcctccc gtccggaagt ggtcaacggg catacggaag
       61 ttatctgctc aaccagtatc caccacattc tggctgtacg aaaaagtaca ctgctgcaaa
      121 tcgacacact tatccggcaa ctggctgaaa tctcagttct gacggaaagc attggaggta
      181 aaaccacccg ggactgggcc atgaaacagg attttcgctg cggctgctgg ctcatggaga
      241 aacctgaaac ggcaatgaaa gccatcaccc gcaaccttga ccgtgaaatc tggcgtgacc
      301 tgatgcaacg ttccaggatg ctttccttaa tggatgcgca ggcccgtgat acatggtacc
      361 ggtcactgga gtacgataat tttccggaaa tcagcgaagc gaacattctg agcacattta
      421 aacaactgca ccagaacaag gatgaggtgt ttgagcgggg agtgatcaac gtcttcaggg
      481 gactgagctg gaattataaa accaactctc cctgtaaatt tggaagtaaa atcattatca
      541 acaatctggt gagatgggac cggtggagat ttcatcttat caccggacaa cagactgacc
      601 gactcgccga tctggaaaga atgctacatc tgttcagcga caaaccgatt cccgacaacc
      661 gggaaaacat cactattcgt ctggatggtc acatccagtc cgttcagggt aaagagcgct
      721 atgaagacga aatgttcagc atcagatact ttaagaaggg ctccgcacac atcacgttca
      781 ggaaaccaga actggttgac aggctaaatg acataattgc caggcactat ccaaatgtat
      841 tagcagacag gtaatgtggc cttgccccgc tattaaacat gtcatatttt tgctatcatt
      901 gtgaaaattt ttcggaggaa acatgactac aaaagaccgt attcagtccc gactgaaccg
      961 ttcaaaacgt tatgtgttca cccgggatga tttcagggat atcgctggct atgatcaggt
     1021 tggcagagct cttcgtacac tggtaaacga aggtaagttg atgaaagtgg gctatggtgt
     1081 ttacaccaaa gcccgtcgta acatcatcac tgggaaaatc atgcccgcag cgcctggggg
     1141 ctctgctgca gtaattacag aggccctgga gcgcctgaat atcagatacc gtctggcagg
     1201 agcaacggaa tgctacaata aaggtcagtc cacacaaatt cctgtatctc tggggataaa
     1261 aacaccttct cgatttaaaa gagtgttaat agtagggaaa agtaaaatca atgcctgaat
     1321 taacatcatt atttcctgac gttgccgatg cactggatat tgaatctgtc gctatcgtcg
     1381 aaaaagatta ttttgtggtt gatcttttaa gattattaaa agaaataaaa ccagaaacac
     1441 atactctcgt gtttgcagga ggtaccgcat tatcaaaagc aggtatctcg ctaaacagaa
     1501 tgtcagaaga tattgatata aagctcgtgc caacagagaa ctttatgcaa aatggcagag
     1561 acaagagaag aaaaattcgc aaggaaatcg tgcaaataat cacggatgtt attcataatt
     1621 ctgacatttt cagcttggat aatgaaaacg cccgtataac acgtgatgag tatcgttata
     1681 acgaaatctc ggtacgttat ccccagacat ttgcacaagt cccctgtctt cgtccattta
     1741 ttaagctgga gttgatggaa agcactctgc tggaacatcc ggaaagcaga gatatttatt
     1801 cccttgtcac ggagttaacg ggtaaaggta cacctgtaac agcattcccc tgtgccacca
     1861 ttttatccac tcaggctgaa aagctcatct ccatgatgcg aagaacagca gcacatcttc
     1921 gtaatcccga acaacaggat gatgagtttc tggtacgtca catttacgac aactactgca
     1981 ttgtcaggga aaaaggtgtg aatgttcctg tattgaaaaa ttttgttcag atatgtattc
     2041 acgctatggt aaccagtacc cacaattttg tagctcacca gttgatgaac ttaagaaagg
     2101 gctggatgca ctccgggatt atcctgtaca caaattgcgc tttcaacgat ttgttaaacc
     2161 gatggtattt ggtaatacac agattaactg ggaagaagcc tattccagtt tcagacaaac
     2221 agcgctaagt gttctgacca gctaacaaac gcgttaagta cgattccaca attcagcata
     2281 tcgaaccaga ccaaatgatt acagcaaagc acaaaaaaga gggattatag cgtcaaaaga
     2341 attcgaattg cacatggggg cc
//
LOCUS       DAPOZO010000217         2347 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPOZO010000217 DAPOZO010000000
VERSION     DAPOZO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.222"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            90..494
                     /gene="ybgC"
                     /locus_tag="ROI21_004787"
     CDS             90..494
                     /gene="ybgC"
                     /locus_tag="ROI21_004787"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDX5113595.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            491..1183
                     /gene="tolQ"
                     /locus_tag="ROI21_004788"
     CDS             491..1183
                     /gene="tolQ"
                     /locus_tag="ROI21_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDX5113596.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSCLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            1187..1615
                     /gene="tolR"
                     /locus_tag="ROI21_004789"
     CDS             1187..1615
                     /gene="tolR"
                     /locus_tag="ROI21_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDX5113597.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            1680..>2347
                     /gene="tolA"
                     /locus_tag="ROI21_004790"
     CDS             1680..>2347
                     /gene="tolA"
                     /locus_tag="ROI21_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDX5113598.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKVEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEA"
BASE COUNT          634 a          535 c          664 g          514 t
ORIGIN      
        1 taacttgccc tcattcccaa acctcaatcg cgcgcgtata gtagcagcgt ttaaaagctc
       61 taacttttgt tgcattaccg ggatgtaaag tgaatacaac gctgtttcga tggccggttc
      121 gtgtctacta tgaagatacc gatgccggtg gtgtggtgta ccacgccagt tacgtcgctt
      181 tttatgaaag agcacgcaca gagatgctgc gtcatcatca cttcagtcaa caggcgctga
      241 tggctgaacg cgttgccttt gtggtacgta aaatgacggt ggaatattac gcacctgcgc
      301 ggctcgacga tatgctcgaa atacagactg aaataacatc aatgcgtggc acctctttgg
      361 ttttcacgca acgtattgtc aacgccgaga atactttgct gaatgaagca gaggttctgg
      421 ttgtttgcgt tgacccactc aaaatgaagc ctcgtgcgct tcccaagtct attgtcgcgg
      481 agtttaagca gtgactgaca tgaatatcct tgatttgttc ctgaaggcta gccttctggt
      541 taaacttatc atgttgattt tgattggttt ttcaatcgca tcctgggcca ttattatcca
      601 gcggacccgt attcttaacg cagcggcgcg cgaagccgaa gcgtttgaag ataaattctg
      661 gtctggaatc gaactctctt gcctctatca agagagccag gggaaacggg ataatctgac
      721 tggttcggaa caaatctttt acagcgggtt caaagagttt gtgcgcctgc atcgtgccaa
      781 tagccatgcg ccggaagccg tagtggaagg ggcgtcgcgt gctatgcgta tctccatgaa
      841 ccgtgaactt gaaaatctgg aaacgcacat tccgttcctc ggtacggttg gctccatcag
      901 cccgtatatt ggtctgtttg gtacggtctg ggggatcatg cacgccttta tcgccctcgg
      961 ggcggtaaaa caagcaacac tgcaaatggt tgcgcccggt atcgcagaag cgttgattgc
     1021 gacggcaatt ggtctgttcg ccgcaattcc ggcggtaatg gcttataacc gtctcaacca
     1081 gcgcgtaaac aaactggaac tgaattacga caactttatg gaagagttta ccgcgattct
     1141 gcaccgccag gcgtttaccg ttagcgagag caacaagggg taagccatgg ccagagcgcg
     1201 tggacgaggt cgtcgcgatc tcaagtccga aatcaacatt gtaccgttgc tggacgtact
     1261 gctggtgctg ttgctgatct ttatggcgac agcgcccatc atcacccaga gcgtggaggt
     1321 cgatctgcca gacgctactg aatcacaggc ggtgagcagt aacgataatc cgccagtgat
     1381 tgttgaagtg tctggtattg gtcagtacac cgtggtggtt gagaaagatc gcctggagcg
     1441 tttgccacca gagcaggtgg tggcggaagt gtccagccgt ttcaaggcca acccgaaaac
     1501 ggtctttctg atcggtggcg caaaagatgt gccttacgat gaaataatta aagcactgaa
     1561 cttgttacat agtgcgggtg tgaaatcggt tggtttaatg acgcagccta tctaaacatc
     1621 tgcgtttccc ttgcttgaaa gagagcgggt aacaggcgaa cagtttttgg aaaccgagag
     1681 tgtcaaaggc aaccgaacaa aacgacaagc tcaagcgggc gataattatt tcagcagtgc
     1741 tgcatgtcat cttatttgcg gcgctgatct ggagttcgtt cgatgagaat atagaagctt
     1801 cagccggagg cggcggtggt tcatccatcg acgctgtcat ggttgattca ggtgcggtag
     1861 ttgagcagta caaacgcatg caaagccagg aatcaagcgc gaagcgttct gatgagcagc
     1921 gcaagatgaa ggaacagcag gctgctgaag aactgcgtga gaaacaagcg gctgaacagg
     1981 aacgcctgaa gcaacttgag aaagagcggt tagctgctca ggaacagaaa aagcaggctg
     2041 aagaagccgc aaaacaggcc gagttaaagc agaagcaagc ggaagaagcg gcagcgaaag
     2101 cggcggcaga tgctaaagcg aaggccgaag cggatgctaa agctgcggaa gaagctgcga
     2161 agaaagcggc tgcagacgcg aagaaaaaag cagaagcaga agccgccaaa gccgcagccg
     2221 aagcgcagaa aaaagtcgag gcagccgcgg cggcactgaa gaagaaagcg gaagcggcag
     2281 aagcagctgc agctgaagca agaaagaaag cggcaactga agctgctgaa aaagccaaag
     2341 cagaagc
//
LOCUS       DAPOZO010000218         2336 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPOZO010000218 DAPOZO010000000
VERSION     DAPOZO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.223"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(15..731)
                     /gene="arcA"
                     /locus_tag="ROI21_004791"
     CDS             complement(15..731)
                     /gene="arcA"
                     /locus_tag="ROI21_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDX5113599.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            827..967
                     /locus_tag="ROI21_004792"
     CDS             827..967
                     /locus_tag="ROI21_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113600.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            1367..2053
                     /locus_tag="ROI21_004793"
     CDS             1367..2053
                     /locus_tag="ROI21_004793"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDX5113601.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            2267..2332
                     /gene="thrL"
                     /locus_tag="ROI21_004794"
     CDS             2267..2332
                     /gene="thrL"
                     /locus_tag="ROI21_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDX5113602.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     misc_feature    2268..>2336
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
BASE COUNT          649 a          534 c          539 g          614 t
ORIGIN      
        1 ggtggtaaag ccgattaatc ttccagatca ccgcagaagc gataaccttc accgtgaatg
       61 gtggcgatga tttccggcgt atccggcgta gattcgaaat gtttacgaat acggcggatc
      121 gtcacgtcta cagtacggtc gtgcggtttc agctcacggc cggtcatttt cttcagcagt
      181 tcagcacggg actgaatttt gcctgggttt tcacagaagt gaagcatggc gcggaactcg
      241 ctgcgcggca gcttgtactg ctcgccatca gggccgatca acgaacggct gttgatgtcc
      301 agttcccaac cattgaactt gtagctttca acgctacgac gttcttcgct gacagtaccc
      361 agattcatgg tacgggacag caggttgcgt gcacgaatag tcagttcacg cgggttgaac
      421 ggtttggtga tgtagtcatc tgcaccgatt tcgaggccga gaattttatc gacttcgttg
      481 tcacggccag tcaggaacat caacgcaaca ttcgcctgct cgcgcagttc acgcgctaac
      541 agaagaccgt tcttacccgg cagattgata tccatgatca ccaggttgat gtcatattca
      601 gagaggatct gatgcatttc cgcgccatct gtcgcttcga aaacatcata gccttccgct
      661 tcgaaaatac ttttcaacgt gttgcgtgtt accaactcgt cttcaacgat aagaatgtgc
      721 ggggtctgca tgtttgctac ctaaattgcc aactaaatcg aaacaggaag tacaaaagtc
      781 cctgacctgc ctgatgcatg ctgcaaatta acatgatcgg cgtaacatga ctaaagtacg
      841 taattgcgtt cttgatgcac tttccatcaa cgtcaacaac atcattagct tggtcgtggg
      901 tactttccct caggacccga cagtgtcaaa aacggctgtc atcctaacca ttttaacagc
      961 aacataacag gctaagagga gccggacacc caataaaact acgcttcgtt gacatatatc
     1021 aagttcaatt gtagcacgtt aacagtttga tgaaatcatc gtatctaaat gctagctttc
     1081 gtcacattat tttaataatc caactagttg catcatacaa ctaataaacg tggtgaatcc
     1141 aattgtcgag atttattttt tataaaatta tcctaagtaa acagaaggat atgtagcatt
     1201 ttttaacaat tcaaccgtta gtacagtcag gaaatagttt agcttttttt aagctaagta
     1261 aagggctttt tctgcgactt acgttaagaa tttgtaaatt cgcaccgcgt aataagttga
     1321 cagtggtcac ccggttcgcg gttatttgag caagaagagt ggcaatatgc gtataacgat
     1381 tattctggtc gcacccgcca gagcagaaaa tattggggca gctgcgcggg caatgaaaac
     1441 gatggggttt agcgagctgc ggattgtcga tagtcaggca cacctggagc cagccacccg
     1501 ctgggtcgca catggatctg gtgatattat tgataatatt aaagttttcc cgacactggc
     1561 tgaatcgtta cacgatgtcg atttcactgt tgccaccact gcgcgcagtc gggcgaaata
     1621 tcattactac gctacgccag tagaactggt gccgctgtta gaggaaaaat cttcatggat
     1681 gagccatgcc gcgctggtgt ttggtcgcga agattccggg ctgactaacg aagagttagc
     1741 gttggctgac gttcttactg gtgtgccgat ggtggcggat tatccttcgc tcaatctggg
     1801 gcaggcagtg atggtctatt gctatcaatt agcaacatta atacaacaac cgacgaaaag
     1861 tgacacaacg gcagaccaac atcaactgca agctttacgc gaacgagtca tggcattgct
     1921 gacgactctg gcagtggcag atgacataaa actggtcgac tggttacagc aacgcctggg
     1981 gcttttagag caacgagaca cggcaatgtt gcaccgtttg ctgcatgata ttgaaaaaaa
     2041 tatcaccaaa taaaaaacgc cttagtaagt atttttcagc ttttcattct gactgcaacg
     2101 ggcaatatgt ctctgtgtgg attaaaaaaa gagtgtctga tagcagcttc tgaactggtt
     2161 acctgccgtg agtaaattaa aattttattg acttaggtca ctaaatactt taaccaatat
     2221 aggcatagcg cacagacaga taaaaattac agagtacaca acatccatga aacgcattag
     2281 caccaccatt accaccacca tcaccattac cacaggtaac ggtgcgggct gacgcg
//
LOCUS       DAPOZO010000219         2326 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPOZO010000219 DAPOZO010000000
VERSION     DAPOZO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.224"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(13..1203)
                     /gene="aspC"
                     /locus_tag="ROI21_004795"
     CDS             complement(13..1203)
                     /gene="aspC"
                     /locus_tag="ROI21_004795"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDX5113603.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(1388..>2326)
                     /gene="ompF"
                     /locus_tag="ROI21_004796"
     CDS             complement(1388..>2326)
                     /gene="ompF"
                     /locus_tag="ROI21_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDX5113604.1"
                     /translation="SKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQG
                     NNSEGADAQKGNKTRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYS
                     DDFFVGRVGGVATYRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEF
                     EGFGIVGAYGAADRTDAQEAEFRGQGKKAEQWATGLKYDANNIYLAANYGETRNATPI
                     EGGFANKTQDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFN
                     KNMSTYVDYIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          566 a          594 c          580 g          586 t
ORIGIN      
        1 tgtttttaac gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg
       61 tgtcatcccg gctacgttca cacgaccaga agcaacagca tacacgccaa actcttcgcg
      121 cagacgcagc acttgttctt ttgtcagacc actgaaggag aacatgccgt tctgtttgat
      181 gataaagctg aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg
      241 catacgctga atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc
      301 atcgttgctc aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt
      361 agcgcgaatc gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcagcagc
      421 aaccagagta caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact
      481 ggcaacaatc agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc
      541 cagaccacgg gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac
      601 ggaaagttgt gccagtgtct gccactgttc cagcgtaggg tcgataccgg ttgggttatg
      661 gcagcagcca tggaacagca ctacgtcgcc agcctgagct tcgttcaggc tgttaatcag
      721 tgcatcgaag tcaagggtgt gattttccgc atcataataa gcgtattcac gaacttccag
      781 acctgcagag ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacacacg
      841 cttaacgctg gtattttttg ccaggaaatc ggcagccacg cgtagtgcgc cagtgccacc
      901 cggagtctgt gccgtgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag
      961 cagttcctga gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt
     1021 ggtttcattt tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt
     1081 gcccgtctca tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc
     1141 acgaaacaga tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa
     1201 catgacgagg ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt
     1261 tacaaaattc caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc
     1321 tctggaaggt ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg
     1381 tgtgctatta gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca
     1441 gtttgttgtc agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt
     1501 tgaagtagta ggttgcgccc acttcaaagt agttcaccag atcaacatca ccgatacctt
     1561 ctacgtcttt cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact
     1621 ggtattgcgc aactaacaga acgtcttgcg ttttgttggc gaagccgcct tcaattggcg
     1681 tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg tcatacttca
     1741 gaccagttgc ccactgttca gcttttttac cctgaccgcg gaattcggct tcctgggcgt
     1801 cggtacggtc tgctgcaccg taagcaccta cgataccaaa gccttcgaat tcgtagctga
     1861 tagagccacc gacgccgtcg ccgttggaac gctcaggaat accagcacgt tcgtttttgc
     1921 ccaggtactg aacggcaaag ttcaggccgt caaccagacc gaagaagttg gagttacgat
     1981 aggtagcaac gccgcctaca cgaccaacga agaagtcatc gctgtatgcc gtgtcaccgc
     2041 caaactcagg cagcatatcg gtgtaaccca gtgcgtcgta aaccacaccg tagttacgac
     2101 cgtagtcgat agaacccgca tcagcgaatt tcagacctgc gaatgccaga cgagttttgt
     2161 tacctttctg ggcatcagcg ccttcagagt tgttaccctg gaagttatat tcccactgac
     2221 cgtagccggt cagatcggaa ttgatttgtg tttccccttt aaaaccaaga cgggcataag
     2281 ttttgtcgcc gtttccgccg taactgttta caccattgtc tttaga
//
LOCUS       DAPOZO010000220         2317 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPOZO010000220 DAPOZO010000000
VERSION     DAPOZO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.225"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(36..986)
                     /gene="cbl"
                     /locus_tag="ROI21_004797"
     CDS             complement(36..986)
                     /gene="cbl"
                     /locus_tag="ROI21_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDX5113605.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1088..2005)
                     /gene="nac"
                     /locus_tag="ROI21_004798"
     CDS             complement(1088..2005)
                     /gene="nac"
                     /locus_tag="ROI21_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDX5113606.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
BASE COUNT          622 a          590 c          499 g          606 t
ORIGIN      
        1 tttctgaaaa gcccgctttt atagcgggct ttttgctata tctgataatc aatttcctct
       61 tcactgttct ccatcacctg gcgcttgata tcctcaactg acagtcctgc attacaaagt
      121 tccagaaagc gccagacata gttacgttga agttgccctc gcttcagtcc caaccataca
      181 gtattggcat cgaaaagatg ccgcgtatcc agacggatta aattctcttc ctcttgttcg
      241 ccgctggatt gttcggcaac taatccaatc cccagcccaa gagcaacata ggttttaatg
      301 acatcagaat cctgcgcact taatacaata tctgccagca aacctttgcg ggcaaatgcg
      361 tcatcaatac gtgagcgccc cgtaatcccc tgtcggtaag tgattaacgg ccacttcgct
      421 attgattcca gcgtcaacgg tgtaatttgc gtcaagggat gatcgagtgg aacaagcaaa
      481 ctatggtgcc aacgaaacca cgggaaggcg acgagctgcg ggtcattact caaacgctcg
      541 ctggcgatac caatatcagc ttcgccattt tgcaacaacg tcgcaatttc ctgtggcgta
      601 ccctggatta gctccagccg aacctccggg aaaagttcgc gaaaagcttt aatgacctca
      661 ggcaagctat aacgtgcctg agtatgcgtc gttgcaatag tgagaacgcc agacgtatcg
      721 ttggtaaaca ggtctgcaag ccgacgaaca ttactggctt cattcagaat acgttctgca
      781 atgaccagta atgctttgcc cggttcagtc atgcccagca gtcgcttacc tcgtcgaaca
      841 aatatttcga tgccaagttc atcctccagt tcccgaatat gacggctgac gccggactgt
      901 gaggtaaaaa gcatattcgc aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg
      961 attatcttta gttgttggaa attcacggta aactccgggc agttcagatt tcccgttatt
     1021 gttaaagtct aatgcccggc ataacaaata ataaaaaccc gcatcttatt ccatcccgat
     1081 ataacactta gctcaccaat tgccactgcc ttttttccat cactggagaa ctaatcactg
     1141 acattaacaa ctctttcact gcctgtgcct gtggcgataa gttcgctctg gcgggtaaat
     1201 ttaatgacaa agagagactc atggaaggag tggtaatgcg tgacatccac ccatttactg
     1261 cgccacataa cgaacgcgcg gccgattcgg gtaatactgc aacgcccatg ccgctggcaa
     1321 tcgctgcggt aagcgtggca atagactcaa tttcaccaat aacttttgcc gtgagtcgcc
     1381 gtagggaaaa agcctcatca acacgaagtc taatagcact gtaatcactg gggagaaaga
     1441 ggttcatttg cgcaatagca ttcacatcaa cgctttgccc cgggcaatct tgagttccta
     1501 ccagaaaaag atcttctttc agcaaagcct gactggatac accagccaca ggggaatgct
     1561 cataaatcac cgccatatcg agttggtgat ttatcaattt ttcgttaagc actgcaccac
     1621 tattttcatg aagatagata acgatctccg gaaattcagc gcgaaccgcc tgtaataagg
     1681 gcatggtgat ggatgacgca gcggttcctg gtgcaaagcc aatcgagact tgccccgata
     1741 atgcctgacc aacgttatgc accgccagtt gggcctgttc acactgacgt aaaatggccc
     1801 gcgcatgggt atagagaatt tttccggcgt ctgttggtgt aacgccccgc tttgtacgga
     1861 tcaaaagttg ttgatttaac tcaccttcca gtgtggcaac ctgctggctg agcgctggtt
     1921 gtgcgatatg caatacttca gcagcctggg tcaggctacc aatatctaca atttttacga
     1981 agtatttcag gcgtctgaag ttcatgttgc ctccggtttt taagaatcgg cccaagtgcc
     2041 gccattactt acaaccagat tgcaagatgc ttgccagttt tattttggtg ttgatgtaca
     2101 agctaaccaa ctgtcaaata agagattatg atagattcgt catttgctcc tttaatcagc
     2161 tgtcgcgttc ccctgcccta taaaaggagg gtatgcacca cgatggttca ttacccaata
     2221 agattgaaag ctcaccactt tgttgaaatt gacagcaaac aaacaaaaaa atgcatttca
     2281 ccctttgaca tcaccatgca ctgccattaa tatgcgc
//
LOCUS       DAPOZO010000221         2216 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPOZO010000221 DAPOZO010000000
VERSION     DAPOZO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.226"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..205
                     /locus_tag="ROI21_004799"
     CDS             <1..205
                     /locus_tag="ROI21_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113607.1"
                     /translation="YYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPGQVENRALYP
                     RPEGRGFTAHRIKKQSKSLSKTG"
     gene            166..576
                     /locus_tag="ROI21_004800"
                     /pseudo
     CDS             166..576
                     /locus_tag="ROI21_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            874..1758
                     /gene="tsx"
                     /locus_tag="ROI21_004801"
     CDS             874..1758
                     /gene="tsx"
                     /locus_tag="ROI21_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDX5113608.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(1796..2143)
                     /locus_tag="ROI21_004802"
     CDS             complement(1796..2143)
                     /locus_tag="ROI21_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDX5113609.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
BASE COUNT          581 a          534 c          519 g          582 t
ORIGIN      
        1 ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc agtagctgcg gaggtgcgcc
       61 aatatccgtc atccgccaat acattgaaca acagcaaaca cctggtcagg tggaaaaccg
      121 cgccttatat ccccgccctg aaggacgggg ttttacggcg caccggataa agaaacagtc
      181 aaaatcactc agcaaaacag gctgaatgca aaatccagca gtggggttta ccttctgcct
      241 ggagcgaaaa caccagcaag actggaaagc cagatcggta ctttacgtat gtcgctggtg
      301 aatattacgc ctgatacaga tggcaccacc ctgacgctac gtattcaggg cgagtcgaat
      361 gatcccttgc ccgcattcag cggaactgtt gaatatgggc aaattcaggg aacaatagac
      421 aactttcagg aaatcaatgt gcagaatcaa ttgattaacg cccctgccag cgtcctcgcc
      481 cccagcgatg ttgatattcc gttacagtta aagggtattt ctgtggatca gttaggcttt
      541 gtacgtatcc acgacattca acctgttatg cagtaaacgt ttcgcggggc gttttgtgcg
      601 tcccgcaaca tctttccccg tcattttgtt actctgctta catcacccag attgatagta
      661 aaagtttgca acaagggtga aagtcagtac aatccccgcc cgaatgtgtg taaacgtgaa
      721 cgcaatcgat tacgtaaatg atagaactgt gaaacgaaac atatttttgt gagcaatgat
      781 ttttataata ggctcctctg tatacgaaat atttagaaac gcaatttgcg cctttttcac
      841 tcccgcaagg gattttcaaa cagtggcata catatgaaaa aaacattact ggcagccggt
      901 gcggtactgg cgctctcttc gtcttttact gtcaacgcag ctgaaaacga caaaccgcag
      961 tatctttccg actggtggca ccagagcgtt aacgttgtcg gaagctatca cacccgtttc
     1021 ggaccgcaga tccgcaacga tacctacctt gagtacgaag cattcgctaa aaaagactgg
     1081 ttcgacttct atggttatgc ggatgcgccg gtattcttcg gcggtaactc cgatgcaaaa
     1141 ggtatctgga accacggttc tccgctgttt atggaaatcg aaccacgttt ctccatcgac
     1201 aagctgacca atactgacct tagctttggt ccgttcaaag agtggtactt cgcgaacaac
     1261 tacatttacg acatgggtcg taataaagat ggtcgccaga gcacctggta catgggtctg
     1321 ggtaccgata tcgatactgg cctgccgatg agcctgtcca tgaacgtcta tgcgaaatac
     1381 cagtggcaga actatggcgc agcgaatgaa aacgagtggg acggttaccg tttcaaaatt
     1441 aaatactttg tgccgattac cgatctgtgg ggcggtcagc tgagctacat cggctttacc
     1501 aacttcgact ggggttccga tttaggggat gacagcggta atgcaatcaa cggtattaag
     1561 acccgtacca ataactccat cgcttccagc catattctgg ctctgaacta cgatcactgg
     1621 cactactctg tcgtagctcg ttactggcac gacggtggtc agtggaacga cgatgcagaa
     1681 ctgaacttcg gcaacggcaa cttcaacgtt cgctctaccg gctggggtgg ttacctggta
     1741 gtaggttaca acttctaatt gtgagaatgc cggattccat atccggcatt ttttatcact
     1801 tcttcttatt catcatcgct tttaaatcag cgaatgggtt gtacttcgct tcaccgacat
     1861 ctttctgcgc atcttcccct gcgataacgg tcgtaccata ctgatctgct tcggtatatt
     1921 ttgaatgctc gtgatcgtgg caataaagac acaataactc ccagttactg ccgtcttccg
     1981 ggttattggt atggtcgtga tcaatgtggt gaacggtaag ttcacgcagg ttggaataaa
     2041 caaactcgcg agaacagcga ccgcagaccc acggatagat ttttaatgct ttttcgcgat
     2101 agccgctttc taaccgcgca tagtttttag ggatgatagc catgaaaagt tacctggatg
     2161 aaatgtaggg ttacgcgcag tttcccaaga tgcggggatt ttcgcaatct ccatac
//
LOCUS       DAPOZO010000222         2188 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPOZO010000222 DAPOZO010000000
VERSION     DAPOZO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.227"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..369
                     /locus_tag="ROI21_004803"
     CDS             <1..369
                     /locus_tag="ROI21_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDX5113610.1"
                     /translation="LKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIR
                     NHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMDRNYLASVLAGSGLS"
     gene            388..699
                     /locus_tag="ROI21_004804"
                     /pseudo
     CDS             388..699
                     /locus_tag="ROI21_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(794..1564)
                     /gene="yafV"
                     /locus_tag="ROI21_004805"
     CDS             complement(794..1564)
                     /gene="yafV"
                     /locus_tag="ROI21_004805"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDX5113611.1"
                     /translation="MSGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1745..>2188
                     /gene="ivy"
                     /locus_tag="ROI21_004806"
     CDS             1745..>2188
                     /gene="ivy"
                     /locus_tag="ROI21_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDX5113612.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
BASE COUNT          599 a          548 c          554 g          487 t
ORIGIN      
        1 ctgaagaaat tatgcgtggc agtctccttt cggtcaataa tagcagaaca aaagaaagag
       61 ccagaaatga cggtcagata ttatattagt tctgctgatt taaccgcaga gaagttcgcc
      121 acagcaatcc gaaaccactg gcacgtggag aataagctgc actggcgtct ggacgtggta
      181 atgaatgaag acgactgcaa aataagaaga ggaaatgcag cagaattatt ttcagggata
      241 cggcacatcg ctattaatat tttgacgaat gataaggtat tcaaggcagg gttaagacgt
      301 aagatgcgaa aagcagccat ggacagaaac tatctggcgt cagtccttgc ggggagcggg
      361 ctttcgtaat ctttccctgg gaaggaatgg gatgcacggt acccgcagat aagtcgcagc
      421 tggcgggcaa actggccgaa tgttgccaca ttcttcgtgt acccggaggc gatccgcaag
      481 gttatctaca ccactaacgc aatcgaatcg ctgaatagca tcatcacaca tgcgataaag
      541 aaacggaaag tgttcccgac agatgagtcg gtgaaaaaag tggtgtggct ggctttccag
      601 tcagcgtcgc aaaaatggac gatgccactt caggactggc ggatagggat gagtcgcttt
      661 attatcgagt tcggtgaccg ccttgacggt cacttctgag aaaggcattt acacagaatg
      721 atggacaggc tccgggcttt cgtaatcttg ccctgacccg tagtcttacc ccgcccctaa
      781 acacaacacc cactcaccac aacctaaact catccgcatc ccgccatgcc ggaaactttt
      841 ctctatattc ccgcaatgcc accatcgaca gctccgcatc aatgcgcgtt gcctgatgcg
      901 cgtcggcagt agcgataatc tcgccctgtg gattaatcac ccggctgtca ccgcgataat
      961 ggcagccgtt gccatcgctg ccgacgcgat tgcatcccgc cacatacgcc tgattctcaa
     1021 tcgcgcgggc cgtcagcaat gcctgccagt gcagagagcg cggagcaggc cagttggcaa
     1081 cgtagatggc gaggtcataa tcgttgagat tgcgcgacca caccggaaaa cgtaagtcgt
     1141 agcacaccag cggcaaaata cgccagccgc gccattccac aatcactcgc gcattgcccg
     1201 ctttataatg tagatgctca tctgccatgc ggaacagatg acgcttatca taaaaatgta
     1261 ccgtgccgcc cggctcaacc agcaaaaagc ggttaaccga accagactcc gtttgtaatg
     1321 caacactgcc tgcaatcagc gcattgcact gctgcgcttt ggctgtcatc cagttcacta
     1381 cgtcatcttg tgctagcgac gaagctgccg cttccatggc aaagccgctg gtaaacatct
     1441 ccggtagaac gatcacatcg cgtccggtaa taccttccag ttgacgatca aaatgacgca
     1501 ggttggcagg accatccatc cacaccagtg gttgctgtaa aagcgtaatc ttcaaaccag
     1561 acacggtgta caactccttt atgcgaaggg ttttataact ctaacacctt atcaggcagt
     1621 tgccttagcg cagaataaat tgataacaaa tgctgatatt ggaaatatct gatttgcaaa
     1681 ttatcgtgtt atcgccaggc tttaggaggt taataacatg ggcaggataa gctcgggagg
     1741 aatgatgttt aaggcaataa cgacagtcgc cgctctggtc atcgccacca gtgcaatggc
     1801 gcaggatgat ttaaccatta gcagccttgc aaagggcgaa accaccaaag ctgcgtttaa
     1861 tcagatggtg caagggcata agctgcctgc ctgggtgatg aaaggcggta cttatacccc
     1921 tgcacaaacc gtaacgttgg gagatgagac gtatcaggtg atgagcgcgt gcaaaccgca
     1981 tgactgtggc tcgcaacgta tcgctgtgat gtggtccgag aaatctaatc agatgacggg
     2041 gctgttctcg accattgatg agaaaacgtc gcaagagaaa ctcacctggt tgaatgtgaa
     2101 cgatgcgctt tcgattgatg gtaaaacggt gctgttcgcg gcgttgaccg gtagcctgga
     2161 aaaccatccg gatggcttta attttaaa
//
LOCUS       DAPOZO010000223         2142 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPOZO010000223 DAPOZO010000000
VERSION     DAPOZO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.228"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(106..732)
                     /gene="tetR(A)"
                     /locus_tag="ROI21_004807"
     CDS             complement(106..732)
                     /gene="tetR(A)"
                     /locus_tag="ROI21_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HDX5113613.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLS
                     PLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            838..2037
                     /gene="tet(A)"
                     /locus_tag="ROI21_004808"
     CDS             838..2037
                     /gene="tet(A)"
                     /locus_tag="ROI21_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HDX5113614.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2069..>2142)
                     /locus_tag="ROI21_004809"
     CDS             complement(2069..>2142)
                     /locus_tag="ROI21_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PecM"
                     /protein_id="HDX5113615.1"
                     /translation="GSIWLGQRSNRTPRARIACRKSP"
BASE COUNT          343 a          674 c          669 g          456 t
ORIGIN      
        1 ccgtagcggg ctgctacggg cgtctgacgc ggtggaaagg gggaggggat gttgtctaca
       61 tggctctgct gtagtgagtg ggttgcgctc cggcagcggt cctgatcaat cgtcaccctt
      121 tctcggtcct tcaacgttcc tgacaacgag cctccttttc gccaatccat cgacaatcac
      181 cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt ctatcgcggc
      241 ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg ccggcctgct cctcaagcac
      301 ggccccaaca gtgaagtagc tgattgtcat cagcgcattg acggcgtccc cggccgaaaa
      361 acccgcctcg cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg
      421 tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct
      481 gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt
      541 atgattctcc gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa
      601 gtgccagtaa agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag
      661 accgtctacg ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg
      721 caactttgtc atgcttgaca ctttatcact gataaacata atatgtccac caacttatca
      781 gtgataaaga atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg
      841 aaacccaaca gacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc
      901 ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc
      961 accgcccact atggcattct gctggcgctg tatgcgttga tgcaatttgc ctgcgcacct
     1021 gtgctgggcg cgctgtcgga tcgtttcggg cggcggccgg tcttgctcgt ctcgctggcc
     1081 ggcgctgctg tcgactacgc catcatggcg acggcgcctt tcctttgggt tctctatatc
     1141 gggcggatcg tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc
     1201 gatatcactg atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg
     1261 ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct
     1321 ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg
     1381 ccggagtcgc acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct
     1441 tcgttccggt gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc
     1501 atgcaacttg tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt
     1561 cactgggacg cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc
     1621 gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg
     1681 ctcggaatga ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg
     1741 atggcgttcc cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa
     1801 gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg
     1861 gcgctcacca gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct
     1921 tctataacaa cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc
     1981 ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga tcgctgatcg
     2041 tggaaacgat aggcctatgc catgcgggtc aaggcgactt ccggcaagct atacgcgccc
     2101 taggagtgcg gttggaacgt tggcccagcc agatactccc ga
//
LOCUS       DAPOZO010000224         1941 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPOZO010000224 DAPOZO010000000
VERSION     DAPOZO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.229"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            37..540
                     /locus_tag="ROI21_004810"
     CDS             37..540
                     /locus_tag="ROI21_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113616.1"
                     /translation="MTELTKKQLIEEAKLKIAIEKCHPNSGMARVEGELFKIALASLE
                     AEPVGDFYEYKPDDWYQRSAGDKAPKWTPPYAVFPAPVVPEEMDLLTCHLDGVTETYA
                     EGWNACRAALLQGKGEPGKQVRELTMLVKQLVSQLKKPKPGCKLPDKVMDYLERSGLI
                     SVEDVLR"
     gene            complement(661..1434)
                     /locus_tag="ROI21_004811"
     CDS             complement(661..1434)
                     /locus_tag="ROI21_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDX5113617.1"
                     /translation="MTSFQLSLISREIDGEIIHLRAKDGYINATSMCRTAGKLLSDYT
                     RLKTTQEFFDELSRDMGIPISELIQSFKGGRPENQGTWVHPDIAINLAQWLSPKFAVQ
                     VSRWVREWMSGERTTAEMPVHLKRYMVNRSRIPHTHFSILNELTFNLVAPLEQAGYTL
                     PEKMVPDISQGRVFSQWLRDNRNVEPKTFPTYDHEYPDGRVYPARLYPNEYLADFKEH
                     FNNIWLPQYAPKYFADRDKKALALIEKIMLPNLDGNEQF"
BASE COUNT          551 a          365 c          474 g          551 t
ORIGIN      
        1 atattaggag gcggcccgaa agggcggtaa tgaaaaatga ctgaattaac caaaaagcaa
       61 ttaatcgaag aagctaaatt aaaaatagcg attgagaaat gccaccccaa ttcagggatg
      121 gcacgggtag agggcgaatt attcaaaatt gctctggcat cgctggaagc agagccagtt
      181 ggtgattttt atgaatacaa accggatgac tggtatcagc gttcggctgg agataaagcg
      241 ccaaaatgga cgccacccta tgccgttttt ccagcgcccg tagtgccgga agaaatggat
      301 ttgcttacat gccatctcga tggtgtaact gaaacatatg ctgagggatg gaacgcttgc
      361 cgcgctgccc tgcttcaggg caaaggggag cccgggaaac aagttcgcga attgacaatg
      421 ctggttaaac aattggttag tcaactgaag aaaccgaaac cgggctgcaa attgccggat
      481 aaagtgatgg actacctgga gcgaagcgga cttataagcg tggaggatgt tttacgatga
      541 cctggcctga agcattcaca acggtaggaa ttgcgatggc ggtggcgctg gtggtgtatt
      601 cgatttgccg ctggggttga taatactaaa taaccagccc ttatggggct ggctaatcat
      661 ttagaactgt tcattgccat cgaggttagg tagcataatt ttttcaatca aggcgagagc
      721 ctttttgtcc cggtcagcga agtatttcgg ggcatattgc ggcaaccaaa tattgttaaa
      781 atgttctttg aaatctgcta aatattcatt tgggtacagc cttgcagggt atacgcgacc
      841 atcgggatat tcatggtcat aggtagggaa tgttttgggt tctacgtttc tattatctct
      901 taaccattgt gaaaatactc ttccttgtga aatgtcagga accatttttt ctgggagtgt
      961 gtaccctgct tgttcaagag gtgcaaccaa gttgaatgtc agttcattaa gaatagaaaa
     1021 gtgcgtgtga ggaattctac tacgattaac catgtaccgt tttaaatgta caggcatttc
     1081 tgctgtagtt ctttcccctg acatccattc acgaacccat cttgaaacct ggactgcaaa
     1141 tttgggcgat agccactgcg ctagattaat agcgatgtca ggatgcaccc atgtcccttg
     1201 gttttcaggt cttccacctt taaatgattg aattaactcc gatatgggaa ttcccatatc
     1261 gcgtgataat tcgtcaaaaa actcttgagt agttttgagt ctagtgtaat cagagagcag
     1321 tttgcccgca gttctacaca ttgatgtggc gtttatgtaa ccatctttgg cacgtaaatg
     1381 tattatttcg ccgtcaattt cacgagaaat caaagataat tgaaaggatg tcattgaaaa
     1441 ttcctcaagt tggagagttc tggaacatta aggcgaggtg gcagtaatgc accatctgta
     1501 atcttttgat actgacgtaa tcgggcctga tgtgcaacgc cttgcaagta tgtaactcat
     1561 tgaaaacgat catttttatc tattgacatc aaatggcaaa ctgtcattgt gcggctatgg
     1621 caagtggcaa atgcttataa atcatatgga tataaatagt acagaaagag ttgttttctc
     1681 taggatatga gtatacgccc aatgctgatt ggttgaattt tgttcttgtt ttaaagggta
     1741 agagtttaaa gtgttggcag tgatgtattg gatagttaga attgctgtgg gtgcttgagg
     1801 ctatctgcct cgggcatgaa caccaaaggt agatagagaa aagccccagt taacattacg
     1861 cgtcctgcaa gacgtttaac attaatctga ggccatatct atgcgacaca tagagattag
     1921 cctcttacgg accgaaaggt c
//
LOCUS       DAPOZO010000225         1830 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPOZO010000225 DAPOZO010000000
VERSION     DAPOZO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.230"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            67..273
                     /locus_tag="ROI21_004812"
     CDS             67..273
                     /locus_tag="ROI21_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDX5113618.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            367..969
                     /locus_tag="ROI21_004813"
     CDS             367..969
                     /locus_tag="ROI21_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113619.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
BASE COUNT          487 a          500 c          444 g          399 t
ORIGIN      
        1 gtgaaggtgg cgcgctgttt tcatgaccac accggctggt ttaacaacat ccggaggaac
       61 attctcatgg ctaccccagt ttcgctgatg gatgaccaga tggtcgacat ggcgtttatc
      121 actcaactga ccggcctaac cgataagtgg tttgacaaac tcatcaaaga tgggggcttt
      181 cctgcgccca tcaaaatggg gcgcagctcc cgctggctga aaagtgaagt ggaagcctgg
      241 ctgcaggcgc gtattgcaca gtcccgtccg taatttttaa ccactaaccc ttcgcccaca
      301 gcagacgcaa tctccggcct gccggcggcg ctctgctgcc tgttatcccc cgtgaggaat
      361 atgaaaatga aacaacagta tcagacccgt tacgagctgc tccatgaaaa ctaccagaaa
      421 tggctgaccg gcttcacccg gcacgccgta tcatggggcg tgtgtcatcc gaatatctac
      481 tatttccata atctgacgcc cgggtgggtg tcattcaatg gcgaaaaacc ggagattgcc
      541 atcgttcccc agagtctgca ccggctgatt tatggtccgg acaaacggag ctccccgtcc
      601 ctggatgatg atctggtggt gaatttatgc accagcgaac acctgctggt tcatcatccg
      661 atgctggaag ggatcctgct gtctgaatgt gagcgcctga agcaacattc tctggcaaac
      721 aaactgatca gcctcttccg tcagtttggc ggcacggagt tacgtctcaa actggtctgg
      781 ctctgctggc ttgacttaat gaccggaaac agccttgacg actggacgaa aaacctgaaa
      841 cacaaatcag aaaaagatct ggagcaatgg atcattgcac gtcagggtca gagtgaacca
      901 ctgacaaatc tgatggatca gtacgtgctt atggcatacc gtacatcggt tgatgccgct
      961 cacagttgat gtctgagaac cacagggctg gcagcagaaa accagccctg tggtaaaata
     1021 cagtaatagc ggcgtacatc aggcacgccg ctcactcgtc cacagatcat atacgtcatt
     1081 atactgcgcg ataacggagg gcttcagcac ccggtcattt ctgagcgggt atgaatacca
     1141 ttgatgatgc gaagcagcca cgggcagaaa cgttcttcac aaccaggtgc ctccatcttc
     1201 actgcagtct gaatgtgccg gcttgttatc caccagaaat ggtatgctcc ggtacatgca
     1261 taccccggag actataaacg cactgaatca tagcctccca cctcagcagg aggcagtcag
     1321 ctttcaggcg gaaaaacgtt gcagttaaag atcgtgagaa tcgctgtttc gttgcatagc
     1381 aacccgaaca gttgtagtga aacattttac ctgcatcagc acatggtcag gcgccgtctg
     1441 tatggcgtgg aatactgatg tttcggctga tggcaagcct cattctctgt ggaaacaggg
     1501 gcgcgcatta gcttctcaac cagagagtgt cacaaagaca aatattgctg aaaatcgcat
     1561 tcatcgttca ggcgtgaatg cccggagaca aaagccgctg tccacacagc ccccctctga
     1621 cctgaacaaa aagcatacac acaacaggct cacctgaccc cgaaaaacca taacttcagc
     1681 aataccagtg acaccagcac gttacctcag tgccacatct tcagaccgcg atcaatctga
     1741 aatgtgacta tcggggcaat atccctgctt tgtggtacac gataaccaga aaacatcccc
     1801 tgaaccgcca gccagaaaga ctggccacaa
//
LOCUS       DAPOZO010000226         1811 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPOZO010000226 DAPOZO010000000
VERSION     DAPOZO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.231"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            94..1740
                     /gene="mqo"
                     /locus_tag="ROI21_004814"
     CDS             94..1740
                     /gene="mqo"
                     /locus_tag="ROI21_004814"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDX5113620.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          446 a          432 c          529 g          404 t
ORIGIN      
        1 aaacattatt taaatattgt tacttaagtg ttaccgttga tgccgcgcaa atctcacctg
       61 caataaagcg actaaaagta aggcattaac aagatgaaaa aagtgactgc catgctcttc
      121 tcgatggccg tggggcttaa tgccgtttcg atggcggcaa aagcgaaagc gtccgaggag
      181 caggaaactg atgtactgtt gattggcggc ggcattatga gcgccacgtt ggggacctat
      241 ttacgcgagc tggagcctga atggtcgatg accatggtgg agcgcctgga gggtgtcgcg
      301 caggagagtt cgaacggctg gaacaacgcc ggaaccgggc attctgcact gatggaactc
      361 aattacactc cgcaaaacgc cgatggcagc atcagtattg aaaaagcagt cgccattaac
      421 gaagcatttc agatttcccg ccagttttgg gcacaccagg tcgagcgcgg cgtgctgcgt
      481 actccgcgtt catttatcaa taccgttccg catatgagct ttgtctgggg cgaggataac
      541 gtcaatttcc tgcgcgcccg ttacgccgcg ttacaacaaa gctcgctgtt tcgtggtatg
      601 cgttactctg aagatcacgc gcagatcaaa gagtgggcac cgttagtgat ggaagggcgc
      661 gatccgcaac agaaagtggc agccacgcgt acggaaatcg gtactgatgt gaactatggc
      721 gagatcaccc gccagttaat tgcttccttg cagaagaaat ctaatttttc gctgcaactc
      781 agcagcgaag tccgcgccct aaagcgtaat gacgataaca cctggaccgt cactgttgcc
      841 gatctgaaaa acggcactgc acagaacatc cgcgccaaat ttgtctttat cggcgcgggc
      901 ggtgcggcgc tgaagttgtt acaggaatcg gggattccgg aagcgaaaga ctacgccggt
      961 ttcccggtgg gcggtcagtt ccttgtttcg gaaaacccgg acgtggttaa tcaccatctg
     1021 gcgaaggttt acggtaaagc atccgttggc gcaccaccga tgtcggttcc gcatatcgat
     1081 acccgtgttc tggacggtaa acgcgtcgtg ctgtttgggc catttgccac cttctcaacc
     1141 aaattcctca aaaacggttc attgtgggat ctgatgagtt ccaccaccac ctctaacgtg
     1201 atgccgatga tgcacgtcgg gctggataat ttcgatctgg ttaaatatct ggtgagtcag
     1261 gtgatgttga gtgaagagga tcgttttgaa gcgttgaaag agtactatcc gcaggcgaaa
     1321 aaagaggact ggcgtttgtg gcaagcgggg cagcgcgtgc agattatcaa gcgtgatgca
     1381 gagaaaggtg gcgtactgcg tctgggtacc gaagtcgtca gtgaccagca agggactatt
     1441 gccgcgctgc tgggggcatc gccaggggcg tcaaccgccg cgccgattat gttggatctg
     1501 ctggaaaaag tatttggtga tcgcgtttcc agcccgcaat ggcaggctac gttgaaagcg
     1561 atcgttccgt cttatggacg caagctgaac ggtgatgtag cggcaacgga gcgcgagttg
     1621 cagtacacca gcgaagtgct ggggctgaaa tacgacaagc cgcaggcagc agatagtacg
     1681 ccgaaaccgc agttgaaacc gcaacccgtt caaaaagaag tggcggatat tgcgttgtga
     1741 tgatgtgcca catccggcat ggtatgccgg atgtggcgta tgcagataag acgcgtcagc
     1801 gtcgcatcag t
//
LOCUS       DAPOZO010000227         1811 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPOZO010000227 DAPOZO010000000
VERSION     DAPOZO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.232"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            120..1784
                     /gene="glnS"
                     /locus_tag="ROI21_004815"
     CDS             120..1784
                     /gene="glnS"
                     /locus_tag="ROI21_004815"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDX5113621.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYHGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
BASE COUNT          454 a          468 c          475 g          414 t
ORIGIN      
        1 atcaatcatc cccataatct cagtgagatt ttcgattttc actaactaac agttgtcagc
       61 ctgtcccgct tataagatca tacgccgtta tacgttgttt acgctttgag gaatccacga
      121 tgagtgaggc agaagcccgc ccgactaact ttattcgtca gatcatcgat gaagatctgg
      181 ccagtggtaa gcacaccaca gtacacaccc gtttcccgcc ggagccgaat ggctatctgc
      241 atattggcca tgcgaaatct atctgcctga acttcgggat cgcccaggac tataaaggcc
      301 agtgcaacct gcgtttcgac gacactaacc cggtaaaaga agatatcgag tatgttgagt
      361 cgatcaaaaa cgacgtagag tggttaggtt ttcactggtc tggtaacgtc cgttactcct
      421 ccgattattt tgatcagctc cacgcctatg cgatcgaact gatcaataaa ggcctggcgt
      481 acgttgatga actgacaccg gaacagatcc gcgaataccg cggcaccctg acgcagccgg
      541 gtaaaaacag cccgtaccgc gaccgcagcg ttgaagagaa cctggcgctg ttcgaaaaaa
      601 tgcgtaccgg tggttttgaa gaaggtaaag cctgcctgcg tgcgaaaatc gacatggcgt
      661 cgccatttat cgtaatgcgc gatccggtgc tgtaccgcat taaatttgct gaacaccacc
      721 agactggcaa caagtggtgc atctacccga tgtacgactt cacccattgc atcagcgatg
      781 cgctggaagg tattacgcac tctctgtgta cgcttgagtt ccaggacaac cgtcgcctgt
      841 acgactgggt gctggacaac atcaccattc ctgttcaccc gcgccagtac gaattctcgc
      901 gcctgaatct ggaatacact gtgatgtcca agcgtaagct gaacctgctg gtgaccgaca
      961 agcacgttga aggctgggat gacccgcgta tgccgaccat ttccggtctg cgtcgtcgtg
     1021 gttacactgc ggcttctatt cgtgagttct gcaaacgcat cggtgtgacc aagcaggaca
     1081 acaccattga gatggcgtca ctggaatcct gcatccgtga agatctcaac gaaaatgcgc
     1141 cgcgcgcaat ggcggttatc gatccggtga aactggttat cgaaaactac catggcgaag
     1201 gcgaaatggt caccatgccg aaccatccga acaaaccgga aatggggagc cgtcaggtgc
     1261 cgtttagcgg tgagatttgg atcgatcgcg ccgatttccg cgaagaagct aacaagcagt
     1321 acaaacgtct ggtgctgggt aaagaagtgc gtctgcgtaa tgcttacgtc attaaggctg
     1381 aacgcgtgga gaaagatgcc gaaggcaata tcaccaccat cttctgtact tatgacgccg
     1441 acaccttaag caaagatccg gcagatggtc gtaaagtcaa aggtgttatt cactgggtga
     1501 gcgcggcaca tgcgctgccg gttgaaatcc gcctgtacga tcgtctgttc agcgtgccga
     1561 acccaggtgc tgcggatgat ttcctgtcgg tgattaaccc ggaatcgctg gtgatcaaac
     1621 agggctttgc tgaaccgtct ctgaaagacg cggttgcggg taaagcattc cagtttgaac
     1681 gtgaaggtta tttctgcctc gacagccgcc attccactgc cgaaaaaccg gtatttaacc
     1741 gcaccgtagg gctgcgtgat acctgggcga aagtaggcga gtagttttaa gttctaccag
     1801 gccgggtgag g
//
LOCUS       DAPOZO010000228         1786 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPOZO010000228 DAPOZO010000000
VERSION     DAPOZO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.233"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..710
                     /gene="cpoB"
                     /locus_tag="ROI21_004816"
     CDS             <1..710
                     /gene="cpoB"
                     /locus_tag="ROI21_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDX5113622.1"
                     /translation="PISSVGSGSVEDRVTQLERISNAHSQLLTQLQQQLSDNQSDIDS
                     LRGQIQENQYQLNQVVERQKQILLQIDSLSSGGAAAQSTSGDQSGAAASTTPTADAGT
                     ANAGAPVKSGDANTDYNAAIALVQDKSRQDDAMVAFQNFIKNYPDSTYLPNANYWLGQ
                     LNYNKGKKDDAAYYFASVVKNYPKSPKAADAMFKVGVIMQDKGDTAKAKAVYQQVISK
                     YPGTDGAKQAQKRLNAM"
     gene            875..950
                     /locus_tag="ROI21_004817"
     tRNA            875..950
                     /locus_tag="ROI21_004817"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:908..910,aa:Lys,seq:ttt)
     gene            1086..1161
                     /locus_tag="ROI21_004818"
     tRNA            1086..1161
                     /locus_tag="ROI21_004818"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1119..1121,aa:Val,seq:tac)
     gene            1164..1239
                     /locus_tag="ROI21_004819"
     tRNA            1164..1239
                     /locus_tag="ROI21_004819"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1197..1199,aa:Lys,seq:ttt)
     gene            1291..1366
                     /locus_tag="ROI21_004820"
     tRNA            1291..1366
                     /locus_tag="ROI21_004820"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1324..1326,aa:Val,seq:tac)
     gene            1370..1445
                     /locus_tag="ROI21_004821"
     tRNA            1370..1445
                     /locus_tag="ROI21_004821"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1403..1405,aa:Lys,seq:ttt)
     gene            1592..1667
                     /locus_tag="ROI21_004822"
     tRNA            1592..1667
                     /locus_tag="ROI21_004822"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1625..1627,aa:Lys,seq:ttt)
BASE COUNT          493 a          431 c          463 g          399 t
ORIGIN      
        1 caccaatcag tagtgtcggc tcaggctcgg tcgaagaccg cgtcactcaa cttgagcgta
       61 tttcaaatgc tcacagccag cttttaaccc aactccagca acaactctct gataatcaat
      121 ccgatattga ttccctgcgt ggccagattc aggaaaatca gtatcaactg aatcaggtcg
      181 tggagcggca aaagcagatc ctgttgcaga tcgacagcct cagcagcggt ggtgcagcgg
      241 cgcaatcaac cagcggtgat caaagcggtg cggcggcatc aacgacgccg acagctgatg
      301 ctggtactgc gaatgctggc gcgccggtga aaagcggcga tgcaaacacg gattacaatg
      361 cagcaattgc gctggtgcag gataaatccc gccaggatga cgcaatggtg gcatttcaga
      421 atttcatcaa aaattaccct gattcaactt acctgccaaa cgccaattat tggctgggtc
      481 agttaaacta caacaagggt aaaaaagatg atgcggcgta ctattttgct tcggtagtaa
      541 aaaactatcc gaagtcacca aaggctgcag atgcgatgtt taaagtcggc gtcatcatgc
      601 aggacaaagg tgacaccgca aaagcgaaag ccgtatacca gcaggttatc agtaaatacc
      661 ctggtactga tggcgctaaa caggcacaaa aacgtctgaa cgcgatgtaa tgcataacac
      721 acgaccagaa gtcgcattat ttctggtcgt gtcgtgcgaa tcataagcag ttgagtgatc
      781 tacatcgaaa tttttgttgc gctcaagtct gaaatcagta atatatgccg ccgttgccac
      841 gggatatcaa acaaaccgaa agcaacgaaa aagtgggtcg ttagctcagt tggtagagca
      901 gttgactttt aatcaattgg tcgcaggttc gaatcctgca cgacccacca atcgctaagg
      961 tggaagcggt agtaaaacgt gaaggataac gttgcgtcag caacggcccg aagggcgaga
     1021 cgaagtcgag tcatcctgca cgacccacca ctaacctagt tagttgtagt atccagcgta
     1081 gtatcgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
     1141 cgatcccgtc atcacccacc accgggtcgt tagctcagtt ggtagagcag ttgactttta
     1201 atcaattggt cgcaggttcg aatcctgcac gacccaccac taattacggt gggttcggtg
     1261 gaagtagttt gtagtatcca gcgcagtatc gggtgattag ctcagctggg agagcacctc
     1321 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccactcg ggtcgttagc
     1381 tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc
     1441 caccaatttt aacatcaaac tcagatgtta agcgtgaagg ataacgttgc gccggcaacg
     1501 gcccgtaggg cgagcgaagc gagtcatcct gcacgaccta ccaattttaa agattggttc
     1561 cgagtagaaa tctttcaggt aacacccgta tgggtcgtta gctcagttgg tagagcagtt
     1621 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatt taaaggtggt
     1681 tactggtaga gaacgtgaag gataacgttg cgttagcaac ggcccgaagg gcgagacgaa
     1741 gtcgagtcat cttgcacgac ccaccatcct gaatgattaa agcagc
//
LOCUS       DAPOZO010000229         1728 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPOZO010000229 DAPOZO010000000
VERSION     DAPOZO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.234"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..512
                     /locus_tag="ROI21_004823"
     CDS             <1..512
                     /locus_tag="ROI21_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HDX5113623.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPISEIDPQGLNPLILSVVALM
                     SCGLFSSGGVIQSGQQRRDQYGRKVNPEADKLTDEHKSGVNPGGNCSPQNLSDLQNEK
                     NRLCNQSRACAPQMLRKEILRRYEINLACASVRKQINNQCFGGGDKAHMEEENKAYKT
                     AADCSGLIK"
     gene            509..1012
                     /locus_tag="ROI21_004824"
     CDS             509..1012
                     /locus_tag="ROI21_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113624.1"
                     /translation="MKKIIFSNESAVYDELMIHFPCQPLPHISNDITGLEELDIVYNF
                     FQKKQWNEIANNFKIKDDSYALELGITFLPEKVFCYYIPLYIYASLFNKNDFWVFESD
                     FIQQYLCPEYRDHDDFLNFVFNFSDIQLSIIAQFMSYESDAGFFYASKACMDFWEDYS
                     PLLHKKI"
     gene            1077..1682
                     /locus_tag="ROI21_004825"
     CDS             1077..1682
                     /locus_tag="ROI21_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HDX5113625.1"
                     /translation="MTLAYRSLRIIHLYHCDYRGLPLTLISPDGATEWCAEYDEWGNL
                     LTEENPQHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRCITQDPIGLEGGWNQYVY
                     ASIHPTYSIDPLGLIDKPAPVFNRELNSDAYYLAVNNCYSYALNRYGNPGSRIFGGGG
                     LQPGELSGKEFSKLTCSSIFEASKNDGAKDRERSSEVHFLY"
BASE COUNT          537 a          302 c          377 g          512 t
ORIGIN      
        1 aggggcggta tatcactcag gatccgattg gactgaaggg gggatggaat ttttatcagt
       61 atccgttaaa tccgatctca gagatagacc cacaagggtt aaatccgtta attcttagtg
      121 ttgtagcact gatgagttgc ggtttgtttt catcaggtgg tgttatccaa agtgggcaac
      181 aacgcagaga tcaatatggc cgtaaagtta atccagaagc cgacaaatta acagatgaac
      241 ataagtcagg tgtaaatcct ggtggtaact gtagtcccca gaacctgagt gacttacaaa
      301 atgaaaaaaa cagattatgt aaccagtcta gagcttgcgc tcctcagatg ctgagaaaag
      361 aaatattgag acggtacgag ataaatttgg cgtgcgcttc ggtcagaaag cagattaata
      421 atcagtgctt tggtggtgga gataaggccc atatggaaga agagaataaa gcatataaaa
      481 cagcagctga ttgcagtggc ttaataaaat gaaaaaaata attttctcta acgagagtgc
      541 agtctatgat gaacttatga ttcatttccc atgccagcca ttacctcata tttcaaatga
      601 tattacaggg ctggaggaac tggatattgt atataatttt ttccaaaaga aacaatggaa
      661 tgaaatagcc aacaatttta aaataaagga tgactcttat gctttagagt taggaattac
      721 attcctccct gagaaagtat tctgctatta catccctctg tatatttatg cctcgttgtt
      781 taataagaat gatttttggg tttttgaatc cgattttatt caacaatatt tatgtcctga
      841 gtatcgggat cacgatgatt ttttgaattt tgtttttaat ttttcagata tacaactatc
      901 tattattgca cagtttatgt cttatgaatc agacgctgga ttcttttatg caagtaaagc
      961 ttgtatggat ttttgggaag attattcacc tctattacat aagaaaattt aatcgtaaaa
     1021 ggtataaatg gtttaataaa aaacttgtct actcgttgga gtagatgaaa aataagatga
     1081 cgttggcata tagatcactg cgaataatcc acctctacca ctgcgactat cgcggcctgc
     1141 cgctgacgct tatcagccct gacggggcaa cagaatggtg cgcagaatat gatgaatggg
     1201 gcaacctgct gactgaagag aacccgcagc acctgcagca gcttatccgt ctgccggggc
     1261 agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat gacccgctgc
     1321 aggggcggtg tatcactcag gatccgattg ggctggaagg cgggtggaat cagtatgtat
     1381 atgcttccat acatccaacc tattctattg atccgctggg cttaattgat aaacctgctc
     1441 cagtattcaa tcgagaacta aattcagatg cgtattatct tgccgtaaat aattgctatt
     1501 catatgctct aaataggtat ggaaatcctg ggagtaggat ttttggcggc ggaggattac
     1561 aacctggaga attatctgga aaagagtttt ctaaactcac ttgtagtagt atttttgaag
     1621 cgtcaaaaaa tgatggtgcc aaggacaggg aaagatcatc agaagtacac tttttgtact
     1681 aaataattcg cattttatgt ttaaaaattg agatattcct cattacct
//
LOCUS       DAPOZO010000230         1654 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPOZO010000230 DAPOZO010000000
VERSION     DAPOZO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.235"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1057)
                     /gene="ag43"
                     /locus_tag="ROI21_004826"
     CDS             complement(<1..1057)
                     /gene="ag43"
                     /locus_tag="ROI21_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDX5113626.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVL"
     gene            complement(1430..>1654)
                     /locus_tag="ROI21_004827"
     CDS             complement(1430..>1654)
                     /locus_tag="ROI21_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDX5113627.1"
                     /translation="LPDHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESV
                     CIASVACTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          351 a          492 c          410 g          401 t
ORIGIN      
        1 tcagcacatc caggcgtccg ccattttcca gtacgacatt atcagcttta ccctccacaa
       61 cagagaatgc tcccaggcgg tttatgccgg taacggttgc agccgtactg gtaaccagtg
      121 cgccgccctg cttcagggtg acattcgtgg ctgtaccacc ggcgtccacc tgcagtctgc
      181 ccttctgatt aacggtggta ttcccggcca cacccccgtt tttgacaatc tgccagccgt
      241 cactgtttac aacagtgcca gacgccgtac cgccgttgtg cacatactgg taccccccat
      301 tcagcgtggt atccagtgcg tgaccatgta cagtctggtc gccgccggca taaaccacag
      361 tggtatttgc cgttccttca gcagccacaa tctgacgacc atttttattg atggtggtgc
      421 gtacggcatc tccgtagacg gtctgcccgg tatcaccatt ttccgcatcc ggtccccctt
      481 ccgcgccggt attcacaacc gtgtcggttg ccactgcgcc ggatttgatg gcctgccagc
      541 ccttctcatt aatgacggta cccgttgcaa tcccgccttc atgtacccac tgctcaccgc
      601 cgttcagagt ggtgttcact gcctgcccct gaaggctctg tccgcctccg gcactgataa
      661 ccgtgtctga aacgcttcct ccggcattca ctctctgaag accaccaccg gtgacagtag
      721 tgttgttggc gataccgcca ttttgtatcc attgtccgcc ggtattggcc tcgttatccg
      781 gcccatactc cagaccggta ctgatggtca ttccgttggc cgtaccgagg acaatctggt
      841 tgtcatgatt tgtcagtgtt ccgtcgttca cggtttctcc cgcctgtaca accttgtcag
      901 cagccagtgc cgggactgat gtgacagcag caagagacag cgcaaccgcc acaccagcgc
      961 gttttccccg tgagcgggcc agttcggagg ccaccaccag ggtgcccgta atgtgattcc
     1021 ataccagcct gtagctggtg ttcagatgtc gtttcatcag cttttcctta cagagggtga
     1081 ataaaaaagc cggtacccac aaatgtggat accggcaggt acaaaacagc atgctcagat
     1141 aaagtggttt ttcccggaca taaatgcttc ccctctctca tccgccagag aagtatcagt
     1201 ccgttgtgtg caattccgtt ctgcatgatg aagagattca gctgcctttt cgcagaccat
     1261 taaccactat aaacgatcga tagtaacgat caatacctgt tttatcgatc gttttattct
     1321 attaataaca aggcggccac gcaagagaaa caggaggggt atgaggtgtt tgtgtcatgt
     1381 tttcagttgg ttaacgcaat gtaatcaggc atttcccctg gcagagtgtt cagaagaaaa
     1441 tccagttcca tacagcgcgg gcaacagaga ccaccgtgtc acggacggca cgcagaaccg
     1501 tgcatgcaac agaggcaata cagacgctct ccgccgtgtc aaatatccgg tccaccgcac
     1561 cggtaaactg ttcacgggcc tgagcgcgga cagattccgt gcgcagctcg tcctgcaggc
     1621 gggtcatcag gggactggcg gcatggtcgg gaag
//
LOCUS       DAPOZO010000231         1641 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPOZO010000231 DAPOZO010000000
VERSION     DAPOZO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.236"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..292
                     /locus_tag="ROI21_004828"
     CDS             <1..292
                     /locus_tag="ROI21_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDX5113628.1"
                     /translation="QDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVL
                     LNILASQLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(413..985)
                     /locus_tag="ROI21_004829"
     CDS             complement(413..985)
                     /locus_tag="ROI21_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113629.1"
                     /translation="MQKLENRCELLLIQHQKWMTNVTRLIVAHGMGAPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(1071..1277)
                     /locus_tag="ROI21_004830"
     CDS             complement(1071..1277)
                     /locus_tag="ROI21_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDX5113630.1"
                     /translation="MTTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT          398 a          391 c          459 g          393 t
ORIGIN      
        1 gcaggacgcc cgggagctgc tcgactggct cattgaggag tgccgcggag aatgcccgcc
       61 ccgtgtcagg aaaaattata ttctccagtg tggcccgggc cggttccgga acaggaaaaa
      121 gctgaacgtc ctgctgaaca ttcttgcatc tcaactcagg ctctctgtcg tgcaggaagg
      181 aaaaaccacc tatgtcctgc tttccgaaat aaacaatatc acgctgcctg aactgaatgc
      241 gtgctacagc cagaaactga taagatggca tgagcaatcc tttggcttat aatgttccct
      301 gttgctgaga cggattattc agtggtggta tcgcagtatg cccgtcaccc ccgcttctgc
      361 atgagggtga cggaggaaaa gtaagtgtgc ggaggcccgg gagcgttatg aatcaacagg
      421 cgtataacag gtactcgtca atcatcagtt ccagctcgcg cttttcccgt ttgcgcgcca
      481 cagcccagtt ttcaagctct gcatgtgatt tatgctttaa ctcttcagtc cagggctgca
      541 tggagtgggc acacatcaga tcataccagc acagccatat cagcgtgtgg tagagatcac
      601 gatcgtcgaa acgccggagc aggctgttca gcctgtttgc cagccggttt tcacccagac
      661 gatgtagctc actgagtaaa acgccgccca gattaaaatg tgtaagcagc atgatttcat
      721 gggtctgaat gctggtcatt aacccttcct gaatggcagg aatgaacggt ggtgttcccg
      781 ggttgacgac ctgatagagt ccctgtggtg ccactgagat gatggtgcct ttttctggca
      841 gaaaaaagtg ggccagggtc aggcggtgat aaccatgaag atgcggagcc cccattccat
      901 gagcgacgat aagcctcgtc acgtttgtca tccatttctg atgctggatg agcagcagct
      961 cacaccggtt ttcaagtttt tgcataatat tctctccttt agcgcgcagc aaaatgcctc
     1021 cgggggccgg atcatgcctt tgctgcggtt gaacggataa cggtcaggaa ttacggacgg
     1081 gactgtgcaa tacgcgcctg cagccaggct tccacttcac ttttcagcca tcgggaactg
     1141 cggcccagct tgatgggggc cggaaaggcc ccatccctga tgagtttgta aaaccactta
     1201 tcggtcaggc cagtcagttg agtgataaac gccatgtcga ccatctggtc atccatcagt
     1261 gaaactgggg tggtcatgag aatgttcctc cggatgttgt taatctcaac cggcgttggt
     1321 gatgaaaaca acgcaccacc ttcaccggca taacaaccgg ggagaaacgt aaaaaataat
     1381 cgggaatgtg accgtcagga gataaacggg gggattgcgg tggctgagcc gtaccggtat
     1441 gtcgggtggt tgttgcgaaa cgatgcttcc gcacgcgata aaggagggag gactttcgtg
     1501 agacaagggg aaaactagcg ttctgataat tgcagagttg tttttgaagg cggttgtggt
     1561 atatcagcct gtgacctgtg ggcaggcgac agttatcact ggtgggataa gactgtctga
     1621 ttggtattat aaatatataa g
//
LOCUS       DAPOZO010000232         1629 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.237, whole genome shotgun sequence.
ACCESSION   DAPOZO010000232 DAPOZO010000000
VERSION     DAPOZO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.237"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            92..502
                     /gene="mscL"
                     /locus_tag="ROI21_004831"
     CDS             92..502
                     /gene="mscL"
                     /locus_tag="ROI21_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDX5113631.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(499..717)
                     /gene="arfA"
                     /locus_tag="ROI21_004832"
     CDS             complement(499..717)
                     /gene="arfA"
                     /locus_tag="ROI21_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDX5113632.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(773..1198)
                     /gene="zntR"
                     /locus_tag="ROI21_004833"
     CDS             complement(773..1198)
                     /gene="zntR"
                     /locus_tag="ROI21_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDX5113633.1"
                     /translation="MYRIGELAKMADVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSSC"
     gene            complement(1209..1577)
                     /locus_tag="ROI21_004834"
     CDS             complement(1209..1577)
                     /locus_tag="ROI21_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDX5113634.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
BASE COUNT          423 a          395 c          352 g          459 t
ORIGIN      
        1 gattatcagg gattaattaa attcactcct ggcaggaaaa tagcttaaca tttgttagac
       61 ttatgattgt cggcttcata gggagaataa catgagcatt attaaagaat ttcgcgaatt
      121 tgcgatgcgc gggaacgtgg tggatttggc ggtgggtgtc attatcggtg cggcattcgg
      181 gaagattgta tcttcactgg ttgccgatat cattatgcca cctctggggt tattaattgg
      241 cgggatcgat ttcaaacagt ttgctgtcac gctacgcgat gcgcaggggg atatccctgc
      301 tgttgtgatg cattacggtg tcttcattca aaacgtcttt gattttctga ttgtagcctt
      361 tgccatcttt atggcgatta agctaatcaa caaactgaat cggaaaaaag aagaaccagc
      421 agccgcgcct gcaccaacta aagaagaagt attactgaca gaaattcgtg atttgctgaa
      481 agagcagaat aaccgctctt aacaagcgcc tgaaagcaga aggccagtgg taaaaaagtg
      541 atttactttc ttgccactgg cctcccagtt cccccgattg ccatgttttc cttttcgcat
      601 gtaactgcct ttccctttct tatttttctc tactcgctgt cggaataagg gatcatgtag
      661 taatgcttct atcgcattat cctttatctg ccctttagta tgctgatatc ggctcataaa
      721 aactccagtt ggttatttaa cggcgcgagt gtaatcctgc cagtgcaaaa aatcaacagc
      781 tactcttaac gccactcgcc ccttgttcaa gagcttcaag aatcgagcaa taaacactgc
      841 tatgggcggt cccacaacag gcatcgttaa ggcgttgaag ggaacgctgc atactctgca
      901 actcggctat ccgtgcttcg acttcctgca atctttcctg cacaatacct tttgactcct
      961 gacaagtatg gtgttcagga tcgatacgga tcgacagcaa ctcgcggatc gactccagac
     1021 taaaaccaag ttgtctggca tggcggataa atttcaatcg ctggagatcg ctttcggtat
     1081 acaggcgaaa cccaccttcg gtgcgtactt catgctccat catctgctgc ttttcgtaat
     1141 aacgaatcgt gtcgggtgtt acatccgcca tttttgccag ctcaccaatg cgatacatac
     1201 atactccact agttatcgtt gattttgttc aacaacttgt cagcataatc gccgcgtaaa
     1261 aaatcggtac tcaatccagc ttgtcgcagc ttcaattcca gtaatgacaa tcgacggcta
     1321 acctcttgat attgcggatc atcgtgacgg atacctctca ggatatccag aagctgaatt
     1381 gcttccctcc gctgctcaag ttctggcggc aagcaaccgg cattcttcaa caagcgatac
     1441 cccgcacgta attccggtgg cacatgagaa tcatcatcca gtatcaatgg ttcgccgcta
     1501 cctgccaggt tatcaaactc acctttcgct tgcgcttctg ctatatgacg ctctgcccac
     1561 tggtcaagta accacatata tactccaggg gatgaacaaa aagagtacag ctattgtaga
     1621 taagtgggg
//
LOCUS       DAPOZO010000233         1624 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPOZO010000233 DAPOZO010000000
VERSION     DAPOZO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.238"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>1624
                     /locus_tag="ROI21_004835"
     CDS             <1..>1624
                     /locus_tag="ROI21_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDX5113635.1"
                     /translation="DSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQ
                     TDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNRLTAVVYPDGLESRRAYDER
                     DRLVSETSRSGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQT
                     RYEYDRFGQMTAVHREEGISRYRRYDNRGRLTSVKDAQGRETRYEYNAAGDLTAVITP
                     DGNRSETQYDAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQ
                     QRGFDGRTQRYHYDLTGKLTQSEDEGLVTLWHYDESDRITHRTVNGEPAEQWRYDGHG
                     WLREISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHETKQAYNEQGLAN
                     RVTPDSLPPVEWLTYGSGYLAGMKLGDTPLLEYARDRLHRETVRSFGSMAGSNAAYEL
                     TSTYTPAGQLQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLASVRTL
                     APDLDIRIPYATDPAGNRLQDPELHPDSTLTAW"
BASE COUNT          377 a          468 c          562 g          217 t
ORIGIN      
        1 gacagcctga accggcgaga ggtgctgcat acagaaggcg gggccgggct gaagcgggtg
       61 gtgaaaaaag aactggcgga cggcagcgtc acgcacagcg ggtatgacgc ggcaggaagg
      121 ctcacggcgc agacggacgc ggcgggacgg cggacagagt acggtctgaa tgtggtgtca
      181 ggcgacatca cggacatcac cacgccggac gggcgggaga cgaaatttta ctataacgac
      241 gggaaccggc tgacggcggt ggtgtacccg gacgggctgg agagccgccg ggcgtatgat
      301 gagcgggaca ggctggtatc ggagacatcg cgcagcgggg agacagtacg ctaccgctac
      361 gatgacgcgc acagtgagtt accggcgacg acaacggatg cgacgggcag cacccggcag
      421 atgacgtgga gccgctacgg gcagttgctg gcgttcactg actgctcggg ctaccagacc
      481 cgttatgaat acgaccgctt cggccagatg acggcggttc accgcgagga aggtatcagc
      541 cgttaccgcc gctatgacaa ccgtggccgg ttaacctcgg tgaaagacgc acagggccgt
      601 gaaacgcggt atgaatacaa cgccgcaggc gacctgactg ccgttatcac cccggacggc
      661 aaccggagcg agacacagta cgatgcgtgg ggaaaggcgg tcagcaccac acagggcggg
      721 ctgacgcgca gtatggagta tgacgcggca ggacgtgtca tcagcctgac caacgagaac
      781 ggcagtcaca gcgacttcag ctacgatgcg ctggaccggc tggtacagca gcgcggcttt
      841 gacgggcgga cgcaacgtta ccactatgac ctgaccggaa aactcacgca gagtgaagat
      901 gaggggcttg tcaccctctg gcactacgac gaatcggacc gtatcactca ccgcacggtg
      961 aacggcgaac cggcagaaca gtggcggtat gatggccacg gctggctgag agaaatcagc
     1021 cacctgagcg aaggccaccg tgttgccgtc cactatggct atgacgataa gggccgtctg
     1081 accggggagc gccagacggt ggagaacccg gagacggggg aactgctgtg gcagcatgag
     1141 acgaaacagg catacaacga acaggggctg gcaaaccgcg tcacgccgga cagcctgccg
     1201 ccggtggagt ggctgaccta cggtagcggt tatctggcgg gcatgaagct gggcgacacg
     1261 ccgctgctgg agtacgcgcg cgaccgcctg cacagagaaa cggtgcgcag ctttggcagc
     1321 atggcaggca gtaatgccgc atacgaactg accagcacat acacccctgc aggccagtta
     1381 cagagccagc acctgaacag cctggtgtat gaccgtgact acgggtggaa tgacaacggc
     1441 gaccttgtgc gcatcagcgg cccgcgacag acgcgggaat acggctacag tgccacgggc
     1501 aggctggcga gtgtacgcac cctcgcaccg gacctggaca tccgcatccc gtatgccacg
     1561 gacccggcgg gcaaccggct gcaggacccg gagctgcacc cggacagtac actcacggcg
     1621 tggc
//
LOCUS       DAPOZO010000234         1624 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPOZO010000234 DAPOZO010000000
VERSION     DAPOZO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.239"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>1624
                     /locus_tag="ROI21_004836"
     CDS             <1..>1624
                     /locus_tag="ROI21_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDX5113636.1"
                     /translation="DSLNRREVLHTEGGAGLRRVVKKELADGSATHSGYDAAGRLTAQ
                     TDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQVTAVVSPDGLESRRAYDEP
                     GRLVTETSRSGETVRYRYDDAYSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQT
                     RYEYDRFGQMTAVHREEGISRYRRYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITP
                     DGNRSETQYDAWGKAVSTTQGGLTRSMEYDAAGRVTTLTNENGSRSEFTYDVLDRLTE
                     QRGFDGRTQRYRYSATGQLIRSEDEGQVTQWYYDEADRITHRTVNGDPAEQWQYDEHG
                     WLTTLSHTSEGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLAN
                     RVTPDSLPPVEWLTYGSGYLAGMKLGGTPRVEYTRDRLHRETVRSFGSMAGSNAAYEL
                     TSTYTPAGQLQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLASVRTL
                     APDLDIRIPYATDPAGNRLPDPELHPDSTLTVW"
BASE COUNT          375 a          469 c          568 g          212 t
ORIGIN      
        1 gacagcctga accggcgtga ggtgctgcat acagaaggcg gggccgggct gaggcgggtg
       61 gtgaaaaaag aactggcgga cggcagcgcc acgcacagtg ggtatgacgc ggcaggaagg
      121 cttacggcgc agacggacgc ggcgggacgg cggacagagt acggtctgaa tgtggtgtca
      181 ggcgatatca cggacatcac cacaccggac gggcgggaga cgaaatttta ctataacgac
      241 gggaaccagg tgacggcagt ggtgtccccg gacgggctgg agagccgccg ggcatatgat
      301 gaaccgggca ggctggtaac ggagacatcg cgcagcgggg agacagtacg ctaccgctac
      361 gatgacgcgt acagtgagtt accggcgacg acaacggatg cgacgggcag cacccggcag
      421 atgacctgga gccgctacgg gcagttgctg gcgttcaccg actgctcggg ctaccagacc
      481 cgctatgaat acgaccgctt tggtcagatg acggcggtcc accgcgagga aggtatcagc
      541 cgttaccgcc gctatgacaa ccgtggccgg ttaacctcgg tgaaagacgc acagggccgt
      601 gaaacgcagt atgagtacaa cgccgcaggc gacctgactg ccgttatcac cccggacggc
      661 aaccggagcg agacacagta cgatgcgtgg ggaaaagcgg tcagcaccac gcagggcggg
      721 ctgacgcgca gtatggagta tgacgcggca ggacgtgtca ccacgctgac caacgagaac
      781 ggcagccgga gtgaatttac ctacgatgtg ctggaccgcc tgacggagca gcgcggcttt
      841 gatggcagga cacagcgtta ccggtacagc gccacagggc agctcatccg cagcgaggat
      901 gaggggcagg tcacgcagtg gtactacgat gaagcagacc gcatcacgca ccggacggtg
      961 aacggcgacc cggcagagca gtggcagtat gatgagcacg ggtggttaac caccctcagc
     1021 cataccagtg aaggccaccg tgttgccgtc cactatggct atgacgataa aggccgcctg
     1081 accggcgaac gccagacggt ggagaacccg gagacggggg aactgctgtg gcagcatgag
     1141 acgaaacacg catacaacga gcaggggctg gcaaaccgcg tcacgccgga cagcctgccg
     1201 ccggtggagt ggctgaccta tggtagcggc tatctggcgg gcatgaagct gggcgggacg
     1261 ccgcgggtcg agtatacgcg ggacaggctg caccgggaga cggtgcgcag cttcggcagc
     1321 atggcaggca gtaatgccgc atacgaactg accagcacat acacccccgc aggccagtta
     1381 cagagccagc acctgaacag cctggtgtat gaccgtgact acgggtggaa tgacaacggc
     1441 gacctggtgc gcatcagcgg cccgcgacag acgcgggaat acggctacag cgccacgggc
     1501 aggctggcga gtgtgcgcac cctcgcaccg gacctggaca tccgcatccc gtatgccacg
     1561 gacccggcgg gcaaccggct gccggacccg gagctgcacc cggacagtac actcacagtg
     1621 tggc
//
LOCUS       DAPOZO010000235         1617 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPOZO010000235 DAPOZO010000000
VERSION     DAPOZO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.240"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            166..1428
                     /locus_tag="ROI21_004837"
     CDS             166..1428
                     /locus_tag="ROI21_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEc9 family transposase"
                     /protein_id="HDX5113637.1"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDS
                     TNKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFK
                     TTQMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYN
                     IQFAFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEK
                     IIETIESLGCKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRF
                     VVSRVLKPEKERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKY
                     DMAVGHLLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAA
                     KIIKTARYVIMKLSENYPYKGVYEKCLV"
BASE COUNT          605 a          241 c          291 g          480 t
ORIGIN      
        1 tcaaaaagca taatcaaagc cttgataaat atgcattcct tcgaaattca gctttcaccc
       61 attgggtgaa agaaaagtgc tcaaaaatat gttaaattat cagcttttat gactcgatat
      121 atggtaaaat aatagtaaga aaagtagtaa aaaggggttc taattatgat taataaaatt
      181 gatttcaaag ctaagaatct aacatcaaat gcaggtcttt ttctgctcct tgagaatgca
      241 aaaagcaatg ggatttttga ttttattgaa aatgacctcg tatttgataa tgactcaaca
      301 aataaaatca agatgaatca tataaagacc atgctctgcg gtcacttcat tggcattgat
      361 aagttagaac gtctaaagct acttcaaaat gatcccctcg tcaacgagtt tgatatttcc
      421 gtaaaagaac ctgaaacagt gtcacggttt ctaggaaact tcaacttcaa gacaacccaa
      481 atgtttagag acattaattt taaagtcttt aaaaaactgc tcactaaaag taaattgaca
      541 tccattacga ttgatattga tagtagtgta attaacgtag aaggtcatca agaaggtgcg
      601 tcaaaaggat ataatcctaa gaaactggga aaccgatgct acaatatcca atttgcattt
      661 tgcgacgaat taaaagcata tgttaccgga tttgtaagaa gtggcaatac ttacactgca
      721 aacggtgctg cggaaatgat caaagaaatt gttgctaaca tcaaatcaga cgatttagaa
      781 attttatttc gaatggatag tggctacttt gatgaaaaaa ttatcgaaac gatagaatct
      841 cttggatgca aatatttaat taaagccaaa agttattcta cactcacctc acaagcaacg
      901 aattcatcaa ttgtattcgt taaaggagaa gaaggtagag aaactacaga actgtataca
      961 aaattagtta aatgggaaaa agacagaaga tttgtcgtat ctcgcgtact gaaaccagaa
     1021 aaagaaagag cacaattatc acttttagaa ggttccgaat acgactactt tttctttgta
     1081 acaaatacta ccttgctttc tgaaaaagta gttatatact atgaaaagcg tggtaatgct
     1141 gaaaactata tcaaagaagc caaatacgac atggcggtgg gtcatctctt gctaaagtca
     1201 ttttgggcga atgaagccgt gtttcaaatg atgatgcttt catataacct atttttgttg
     1261 ttcaagtttg attccttgga ctcttcagaa tacagacagc aaataaagac ctttcgtttg
     1321 aagtatgtat ttcttgcagc aaaaataatc aaaaccgcaa gatatgtaat catgaagttg
     1381 tcggaaaact atccgtacaa gggagtgtat gaaaaatgtc tggtataata agaatatcat
     1441 caataaaatt gagtgttgct ctgtggataa cttgcagagt ttattaagta tcattgcagc
     1501 aaagatgaaa tcaatgattt atcaaaaatg attgaaaggt ggttgtaaat aatgttacaa
     1561 tgtgtgagaa gcagtctaaa ttcttcgtga aatagtgatt tttgaagcta ataaaaa
//
LOCUS       DAPOZO010000236         1613 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPOZO010000236 DAPOZO010000000
VERSION     DAPOZO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.241"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..1194
                     /locus_tag="ROI21_004838"
     CDS             <1..1194
                     /locus_tag="ROI21_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDX5113638.1"
                     /translation="VQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDC
                     GETRGRWVIEGDLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRA
                     ASEGVPQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVREN
                     WQWKPAVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVND
                     GFIFLGHRIIRKRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNR
                     KLKGWAMFYQFVDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQS
                     KTWVLFGKTNHGKLSGEILYWLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAM
                     AFASI"
     gene            1278..1613
                     /locus_tag="ROI21_004839"
                     /pseudo
     CDS             1278..1613
                     /locus_tag="ROI21_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          408 a          383 c          474 g          348 t
ORIGIN      
        1 gttcagcggg ccatgctgat ggcgatggag ccgatatggg agagtgattt tcatacgctc
       61 tcatatggct tccggcctga gcgcagtgtc caccacgcga tccgcacggt gaaattacag
      121 ctcacagact gtggtgaaac ccggggacgc tgggtgattg aaggcgacct gtccagttac
      181 ttcgacaccg tacatcatcg actgctgatg aaggctgtac gccgcaggat cagtgacgca
      241 cgtttcatga ctctgctgtg gaaaaccatc aaggcgggac atatcgatgt cggtctcttt
      301 cgggcggcca gtgaaggtgt gccacagggc ggtgttatat cgccgctatt atcgaacatc
      361 atgctgaatg agttcgatca atacctgcat gagcgctacc tgagcgggaa agccagaaaa
      421 gatcggtggt actggaataa cagtatccaa cggggccgaa gtacggcggt cagagaaaac
      481 tggcagtgga aacccgcggt ggcgtactgc cgctatgccg atgattttgt cctcatcgtc
      541 aaaggcacca aagcacaggc ggaagccatc agggaggagt gtcggggtgt gctcgaaggc
      601 agtctgaaac tcaggctgaa catggataag actaaaatca cccatgttaa tgacggcttt
      661 atctttctgg ggcacaggat cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc
      721 tcaacgatcc cgcaggagaa agccagaaac ttcgccgcat cgctgacagc actgttatca
      781 ggcaactaca gtgaaagcaa agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc
      841 tgggccatgt tctatcagtt cgttgatttt aaggccaaag tcttcagtta tatcgaccgt
      901 gtcgtgttct ggaagctggc tcactggctg gcccgcaaat accgtacagg tatcgcttcc
      961 ctgatgaggt ggtggtgtaa atcaccgaaa ccgggtcaga gcaaaacgtg ggttttattt
     1021 ggtaaaacca atcacggcaa gctcagcggc gaaatactgt actggttggt ggggcaaggc
     1081 aagaagctgt tccgctggcg gctacccgaa ggtaatccct atctgaggac ggagaccaga
     1141 aacacgtata catcgcgctt tacagaagtg gcaatggcgt tcgccagcat ttaaatggag
     1201 agccggatgc gctgaaaggt gcacgtccgg ttcggggagg agaggcaggg aaatagtccg
     1261 actacgccct gcctcttact ctactcagca catggcaggg ggtcgtctat gtggcgttca
     1321 tcattgatgt gtttgccgga tacatcgtgg ggtggcgggt ctcatcgtct atggaaacga
     1381 cattcgtgct ggatgctctg gagcaggcgt tatgggcccg tcgaccgtcc ggcacagtcc
     1441 atcacagtga taaaggttct cagtatgtat cgctggccta cacacagcgg cttaaggaag
     1501 ccggattact ggcatcaaca ggaagtactg gtgactcgta tgacaacgcg atggcggaga
     1561 gcatcaatgg cctttacaaa gcggaggtaa tacaccgtaa gagctggaaa aac
//
LOCUS       DAPOZO010000237         1608 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPOZO010000237 DAPOZO010000000
VERSION     DAPOZO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.242"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..1038)
                     /locus_tag="ROI21_004840"
     CDS             complement(<1..1038)
                     /locus_tag="ROI21_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDX5113639.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFTNITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTL"
     gene            1514..>1608
                     /locus_tag="ROI21_004841"
     CDS             1514..>1608
                     /locus_tag="ROI21_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDX5113640.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWL"
BASE COUNT          491 a          347 c          413 g          357 t
ORIGIN      
        1 cagagtcagc atgcgtgagt taattttctt ggtatcaata attcggctta acagattgag
       61 aataatgcct ttaatagcct cgtggaaacc gagataaatg ccaaagaatg cggtcagtac
      121 ggcaaagata ttcagcaccg tagaggtgat atgaatgata tgcccaggga tcacctgcgc
      181 ggccagtgcc agtgccgaga tattttgttc aaaggcagaa acggcttctt cgtggctaat
      241 tgagaaagta aacgaaaagg caaaaaacag gatcaccgcg atgagcgtga tataactaat
      301 tcggtgggta cgcagcgcga gccgggttgc cagtacttta tctgcttccc gtttacgata
      361 ggcaatattc attgggttaa gtacctgaat aaatactgca gaaaagaagc aaaatggaat
      421 ggtaagcagg acatcgcgga aaaagacgga ggcttgcggg aaggcggtta tattggtgaa
      481 attccagtgc gggatcatcg caaaaccgaa cacgacgata atccctactt tgaccaccac
      541 cattggcccg gaaatcttaa acagtaatcg ttcaccacca gacgcaattg ccaccagtac
      601 ggcgaaaatg gcgactttat aaagtagaga ttgtgaaaga tcggcatcgg ttaaaccgaa
      661 ggttttcagg tacgaggcgc tgtcgaaaac cacggagaga gagtagataa aaatcccgtg
      721 gataatcatc aaaaagtaga taaccccgag gaaaattccc cagttcttcc ccaggtaatg
      781 actgataata tcggtgtagt cattacagga atcgctttca gaaagggttt ttaaataaat
      841 gtcctgcact acccaggtgg caggataagc aatgatcgct gcggtaataa atacccaaat
      901 tcccttcaag ccaatttgta ctggcatcag cacggttccg gcaccaatcg ccatgcctat
      961 gcataataaa acccagccga agtcgtagcg tgtaaacggc aatttctgat cgtgtttcgg
     1021 tagtgtgttg tgctgcataa agtcctgtcc agaaagaagt cggcctcgca aaaatgaata
     1081 aacccagttt ctcttgcgca acaggtggag aagtcgaata cgcagtatgc aaaaaatgag
     1141 gcaacagatt cactggggag gtcgcgagga ggcgcaattc tacaaaattg cgcatttcac
     1201 taccagtctg gagtcagctc ggatatgaac tgcaactatt gcaaataaag aaattaagct
     1261 ggaaggcatc gcgcaatgag gaagattgcc gggtgctgac ggtaaaaagc aatcgtaaaa
     1321 tcagcggcaa gccggaaagg ttccggtgag gcgcaatgtt gcgggggctt aatccctggt
     1381 ggcattggtt gctggagaga gaaaaccccc gcacgttgca ggtatgcacc tgacaacacc
     1441 acgggggcca ttcttgcatc caaacaatac aagaatagcc gcgaaacgtt gtcattacaa
     1501 cagaggcggc tatatgacgc tcgcagagct gggcatggcc ttctggcatg atttagcggc
     1561 tccggtcatt gctggcattc ttgccagcat gatcgtgaac tggctgaa
//
LOCUS       DAPOZO010000238         1551 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPOZO010000238 DAPOZO010000000
VERSION     DAPOZO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.243"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            353..479
                     /locus_tag="ROI21_004842"
                     /pseudo
     CDS             353..479
                     /locus_tag="ROI21_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            complement(923..1399)
                     /locus_tag="ROI21_004843"
     CDS             complement(923..1399)
                     /locus_tag="ROI21_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDX5113641.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          352 a          414 c          387 g          398 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttatt ccgctacaag cctcgccagc cgtgattttt gccagatttc
      181 acgctctcaa cgctcaacag ctccatcatg gttttgccgt ccgttcaccg ctaaaacgcc
      241 tcagaaatcg ggacaggatg tgcaaatgta ataccgtcac ccgcgacgat attccgtttg
      301 ccgtctggtc agggcttcgc cccgacaccc cgcatcagtg cgtacgtgct caatgtggtt
      361 tccggttctc ttccgcttcc tcgcccactc gctcactacg ttcggtcgct ggggctgcgg
      421 cgagcggtgt cacctctcgc atgttgtcgg tgattccgtt gttgttcttt cggggatgag
      481 tcgccagata gcccctcaaa cgcgctcaga agctctcaga cacacgaaac gggttttaaa
      541 tgcccgtcag ggcatggaag gcgcgaaaaa cgcttacaga ggctctcagg aggtcaggcg
      601 cgccgatacc cctaaccagg ggctttcagg tcgctaggaa ccgcgtcagc cccctgcaaa
      661 gcctatttgc acggcacagg cagctattcg gctggttggt ggtcgtagac cgtcggtgtt
      721 acagcgatgg gggtctgggg ttctccccag acggtttccg gttgcgctca aggcgctgcc
      781 ggaaccgtgg cggcgcggtc gttctgggga tcggggagca aaaacaagca agggattttg
      841 aggcggcaaa agagcaatgc aggatagaca atcatggtca aaacgttaac acgtagcccc
      901 tgattgtccg cctgccgctg gctcaatttg aaaacatggc catgattcgg cgctgaatat
      961 caccgtcttt gttgaccagt gttttagtga caaacgctgc atggtttttc gtagtgttgt
     1021 atcccttcca gacttcatgt ggatcgaggg catacgcaca aaccttacca gcaacgggga
     1081 ttctaactaa tgcatttttt gattctaagt aattgagcca ccgaataatt gtgcttctgc
     1141 taactccaag tttttcagct aggaattgct gatccgcaac gacagcgcca cacgtaggat
     1201 cgatatgctc cgctaaaaat gagtacagct tcagtgctga tatgccttga ctatccttca
     1261 gcagctctcg aacgcgttcc caaccttttg gggatacctg tgtaaacctt gaattcttct
     1321 ttgcttcttc gagatcctcg gtgtttttct ttgcctcatc tgaaagttgt ttcagtcgtt
     1381 tttctgaatc aataaacatg attttttccc tctgaaaatc ccatgcacta acgttggttc
     1441 aaatctaagc catcatgaca catctgtgca acaccttgtt gcgtgttgtc gcatccagga
     1501 tgcgacaacg ttgtgtcacc ctggatgcga caggtaaaca aaaaacacac t
//
LOCUS       DAPOZO010000239         1466 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPOZO010000239 DAPOZO010000000
VERSION     DAPOZO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.244"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            382..>1466
                     /locus_tag="ROI21_004844"
     CDS             382..>1466
                     /locus_tag="ROI21_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDX5113642.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFS"
BASE COUNT          400 a          285 c          349 g          432 t
ORIGIN      
        1 aatattgtac tcagcattca gtgcaggaac tgtagtcttc tttattttat tcttcttcag
       61 gagggggggg gtgattcctg aaggcgttat tcccggtgtt ctgtttatga ttcttcccgg
      121 caggaaaccg gtatctgcaa tacccgggat gatgcttttt gttcgtacgt ggttgtgtaa
      181 aatttgaata gtggatgtgc tccgaaggga aggaggggac acacagattc attttgtctt
      241 tcttcagcag caaaatacac tgtggttatt atttaaccat taaatcaaca aaaaatttta
      301 cctcatattg atttgactgc tatttttgct attaatagcc agaattgcag tgcttttata
      361 tctgtgtata gtgaggttac tatgtcattc ggtcagcctt gtgatgagtt tcccctgagt
      421 tcgctgcctc cactcattcg tgatgccgtt attgaagctc agcaaattac gcaggctccc
      481 cttgggcttg tggcagcgtc tgccctgggc gctgtttcac ttgtctgcca gaatctgatt
      541 gatgtctgca gactgaatac actgcggggg ccggtctctc tttttttcct gacgcttgcc
      601 gagtccggag aacgaaaaac cgcagtggac aaattattaa tgaagcccct gtaccagcag
      661 gagatgcagt tatattccag atacaaaagt gaactggctg tctggaaaaa caaggaagag
      721 cttttaaagg ctcagaaaaa agcattgttg tcaaaactga ataaagaact ccgcaaaggc
      781 gcggatgagt cagaaaccct cagacaactg gaagtacttc agaaaaacag tgcggaagaa
      841 cctgtgaggt ataaattcat ttttaatgat gccacgactg cggcaattaa gaatcagctt
      901 tgtggtaaat ggcgttcagt tggtattatg tcagatgaag ccggtattat atttgatgga
      961 tataccctgt ctgagttgcc ttttattaac aaaatgtggg atggctctgt attgtctgtg
     1021 gacagaaaga atgaacccga acagatgatt gaaaatgcca gaatgacgtt atccctcatg
     1081 gtgcaacccg gtctttttga ccgatatatg gagcgaaaag gttctgttgc cagggacagt
     1141 ggttttcttg cccgttgtct tatcagtaaa cctgcaacca cgcaggggaa acgctttatt
     1201 aacggagctg tgattccggg aggatcatta acggcatttc atgaacgatt aatggaactg
     1261 gccagaggca gtattgaaaa atccagtgag gatgaacggt attgtctgca tttttcgcct
     1321 gaagcgcaaa aaatatttat tgagcactat aatgttctgg agcaggattt atccccttcc
     1381 ggacccttaa gcccgtttcg gggacatgtt tcaaaaaaaa cagaaaacat tgcaagaatt
     1441 gcagcgttat ttcagtattt cagcta
//
LOCUS       DAPOZO010000240         1463 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPOZO010000240 DAPOZO010000000
VERSION     DAPOZO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.245"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            1296..>1463
                     /locus_tag="ROI21_004845"
     CDS             1296..>1463
                     /locus_tag="ROI21_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDX5113643.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVC"
BASE COUNT          370 a          247 c          337 g          509 t
ORIGIN      
        1 caccggaaag gtggcggggg atccacggag ataatatctg tctgaaattg tttggaatcg
       61 atggggatgt tgagtattaa catgaaggag ggcaggagtt atccgttatt gggtgatatc
      121 cttccatgaa aatacgcggt tattgttaaa cagcaggctg attactccgt aaaggagaaa
      181 aatattcatc ttgcagcctg attatcaggt gtgtttatgt ttactgaggt gagtattttt
      241 cagtatattg tcggggcagt cagtgggaga tattaaattt acgtttagag ttatcttgct
      301 tctgctgtgt atttttataa gagtgcgcac gtaatgatat ctgttgatac catgttgtta
      361 agtgtttccc ttccgggtca tgcgtacttg caaaatggca tctgaatcga tcacggtttg
      421 gccaaaccgt gatcgaatag agaggaatac gcacgtaatg attaaatgct tttgttacac
      481 cattcttccg gcatgaatat ttatccagtt ccctttttta tttgtgtagt gatactgtcg
      541 gaatagcgtt cagtaatgtg aagtgtcctg taaaaaaact ttaataaatg tgtcatcctg
      601 gtcaggaaat atctgctgag atattattag ttggtggtgt ttttactgag taatagctgg
      661 cttttaattc ctgaactgaa atactgtcgc tcgtcagggt ttaattaaaa ctggatgatg
      721 atgtgaaaat gaaggctggt ttttgtgttt gtcgaagaag tgttatttta atccattctc
      781 atcattggct ggcgattata cagaagtatt tgaagaaaaa atgttgcatt ttatctgtgt
      841 cagcagggag gaagtatttt attgatgtat cagtattaaa tggtttttgt tatttataaa
      901 aatgtcatcc tgtccataga atattgtact cagcattcag tgcaggaact gtcatcttct
      961 ttattttatt cttcttcagg agggcagttt tctgaaagcg ttactccggg gaaactgttt
     1021 atgtttattc ccggcaggaa acccgtacct gcaataccct ggatgatgct ttttgctggt
     1081 acgttgttgt gtgaaaatta atcaacacgt gtgttccgga gagcgcccgt agacgcacag
     1141 attcattcta acattcttta gcgacaaaat accgaatggt tattatttaa tctgaaaatc
     1201 gacagaaatt tttatcctgt attgattctg ctgctatttt tgctattagt agcaatagtt
     1261 gcggtacttt atgttctgtg tgttgtgagg tcattatgcc atactttcag tatcctgatg
     1321 agtttcccct gagctcgctg cctccactca ttcgtgatgc cgttattgaa gctcagcaaa
     1381 tcacgcaggc tcccctgggg cttgtggcag catctgccct gggcgctgtt tcacttgtct
     1441 gccagaatct gattgatgtc tgc
//
LOCUS       DAPOZO010000241         1456 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPOZO010000241 DAPOZO010000000
VERSION     DAPOZO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.246"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            228..515
                     /locus_tag="ROI21_004846"
     CDS             228..515
                     /locus_tag="ROI21_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113644.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            634..1455
                     /locus_tag="ROI21_004847"
     CDS             634..1455
                     /locus_tag="ROI21_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDX5113645.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
BASE COUNT          420 a          301 c          378 g          357 t
ORIGIN      
        1 acatcccgca aaactttttg cccagaagca ggcaaaaact tttgcggtct gcccccggac
       61 agtcgtttcc gtccgtggca gtgatgcccc ttgcggcgaa ggttctcaaa aaggtgatgg
      121 tgaataccgg agggaacaaa aggcaggtcc ccgcaaaacg tcaaaaccgc ggctcccgaa
      181 actcaacaga tgatgggggc tgaaaaccag agagaggaga aagcaggatg agtacccgaa
      241 atatccacgt taacaccgcg tcgtacacgc ttcttgttgc cgggaagaaa aaaaacaccg
      301 gggaagagtg ggacgtcctg gaattcagca gcctgactga gctgaaaaaa tatcgcaaaa
      361 gccacccgga aaagatggcc ttcagttata gctacgcgct cagtcagggt gtggataagc
      421 agttccgcca tatcaacatt gcggaagccg atcacttcaa acagttcctg cgtcagatta
      481 agcgtgccgg cctcgatatc cgggcgatct gctgaccgga tgtgatgaat acccctcctg
      541 attgttgcaa tcttgttaat ggagatcggg ggggatgata ttaagcggaa aaatttttta
      601 ttaaatttat tctttcataa aggagtagct gttatgcgat tagcttcccg ttttggtcgg
      661 tataattcca tccgccgtga acgtccttta acggatgatg aattaatgca gttcgtgcct
      721 tcggtatttt ccggtgataa acatgagtcc cggagtgaac gttatacgta tattccaaca
      781 atcaatatca tcaataagtt acgtgatgaa ggtttccagc cattctttgc ctgtcagagt
      841 cgggttcgtg atttgggacg tcgcgaatac agtaaacata tgttacgtct tcgcagggaa
      901 gggcatatta acggacagga agttcctgaa attatcctgc ttaattcaca tgatggttca
      961 tccagttatc agatgatccc cggaattttt cgttttgtct gcacaaatgg cctggtgtgc
     1021 ggaaataatt ttggcgaaat ccgcgttcca cataaaggtg atattgtcgg gcaggttatc
     1081 gagggagcgt atgaagtgct cggtgtcttt gataaggtca ctgataatat ggaggcgatg
     1141 aaagaaattc atcttaacag tgacgagcaa catttatttg gcagagctgc actgatggtc
     1201 aggtatgaag atgaaaataa aacgccagtg acacctgaac agataattac tccccgtcgt
     1261 cgggaagaca aacagaacga tctctggaca acctggcagc gggttcagga gaatatgata
     1321 aaaggtggat tatcggggcg aagtgcctcc gggaaaaata ccaggacaag agccattaca
     1381 ggtattgatg gtgatataag aatcaacaag gcgttatggg tgattgccga acagttcaga
     1441 aagtggaagt catgat
//
LOCUS       DAPOZO010000242         1430 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPOZO010000242 DAPOZO010000000
VERSION     DAPOZO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.247"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            103..450
                     /locus_tag="ROI21_004848"
     CDS             103..450
                     /locus_tag="ROI21_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113646.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(470..979)
                     /locus_tag="ROI21_004849"
     CDS             complement(470..979)
                     /locus_tag="ROI21_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113647.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLPAGGTSATDLAV
                     ELNGITYQACRGDFVVRLDGSTCLQLWNKEGRVVRREGDPLEVAQWLQACHDAGMEVR
                     VQINESAAP"
     gene            complement(976..1236)
                     /locus_tag="ROI21_004850"
     CDS             complement(976..1236)
                     /locus_tag="ROI21_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113648.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          298 a          399 c          393 g          340 t
ORIGIN      
        1 tttgtcagtg tactgatgca tgacaacata accacagcat gataataata atcaataaca
       61 ataagctgtg tcacgtttac atcatggaac ggaggtgctc ctgtgacttt tgagcagaaa
      121 aaagcccggg ccattgccct gatggacagc aaaaagatgt ggcggagcaa ttatgcccct
      181 cctcttctgc gtattctctg gcggctgggt atccggcttc cgccattacc ctttatgccg
      241 ttctggcagg tcacggtgct gacggggggc ttatggggca tttcctgggg atgtgcaatg
      301 tggtttatct actgggggcc gtcaggaatg gtggcaggtg aggccattat tataagtatc
      361 acgggggggt ttttgtccgg actgctcatg gcctctttcc actggtggcg caggaaagtt
      421 aaccggctgc caccatggga tgatgtgtaa accgtgcaca ggcgtgttct tacggagcgg
      481 cactttcatt tatctgtacg cgaacctcca ttcctgcatc gtgacaggcc tgcagccact
      541 gcgccacttc cagcggatcg ccctcccggc gtaccaccct gccttcttta ttccataact
      601 gcagacaggt gctgccgtcg agacgcacca caaaatcccc acggcaggcc tgatacgtta
      661 tgccgttcag ctccaccgcc agatcagtgg cagaagtgcc gcctgcgggc agcagaggct
      721 gtgccacggc ccggccggcg tccgtcagct ccactgccag ctgcaggttc ggagcgcgca
      781 gggtacgcag ccagccggca tcctccagac gacggcagga ggcgcgcagg tttggcccgt
      841 agaccggggc ttccccgctt tgctccagca cccgttctat gtcccgcgtc agcacgggtc
      901 ctggccgttt ttcatcaagg gcagccagca caatcagcac tctccgctgc agtggcgacg
      961 gtcggcggct ctgagtcatt tccagtcatc ctccggccag atgagctttc tgaggcgctg
     1021 atggtcggcg tcaatgatct cctgtctctt tatggcattc attgaggtat agccatttat
     1081 ggccagttca tgccagaaga tcagcacggc tccggctttg gctgactcgc agagataagt
     1141 tacgccgtgg ctgtccagat tgcattcccg gatgcgtttt tccgcaaacc gggtcagttg
     1201 ttcatagctg agtgaaaacg tcatttcctg atccatcctg tacgttccat ctgataatgt
     1261 tctgacaggt aaaaatcata tcctgaaccg ccccgggaat cctggagact aaactccctg
     1321 agaaagaggt aaacaggatg actaaaaata ctcgtttttc ccccgaagtc cgtcagaggg
     1381 cgattcgtat ggttctggaa agtcagggcg aatatgactc acagtgggcg
//
LOCUS       DAPOZO010000243         1381 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPOZO010000243 DAPOZO010000000
VERSION     DAPOZO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.248"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            1..416
                     /locus_tag="ROI21_004851"
                     /pseudo
     CDS             1..416
                     /locus_tag="ROI21_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    <1..74
                     /locus_tag="ROI21_004851"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            1089..>1381
                     /locus_tag="ROI21_004852"
     CDS             1089..>1381
                     /locus_tag="ROI21_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113649.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLG"
BASE COUNT          342 a          345 c          417 g          277 t
ORIGIN      
        1 tgatgccact gctggataag ctgcgtaagc tgtacggggt cggaccggta tgcagtgaac
       61 tacatattgc cccgtcaacg tattaccact gtcagcaaca gcgacatcat cctgataaac
      121 gcagtgcccg tgcgcagcgc gatgactggc tgaagaaaga gatactgcgc gtatacgatg
      181 ggaatcatca ggtatacggt gtgcgtaaag tctggcgtca gttgttacgg gaaggaatca
      241 gggtagccag atgtacagtg gcgcgcctca tggcggttat gggacttgcc ggtgttctcc
      301 ggggtaaaaa ggtccgcact accgtcagcc ggaaagccgt tgccgcaggc gaccgcgtaa
      361 accgtcagtt tgtggcagaa cgttctgacc agttgtgggt ggctgatttt acttacggtg
      421 tgcccggagt tcagggcggg catggatgct taaatgaacc gcgagtctgt ctggaatatt
      481 gaaccggtaa ctcacgatga gaaacccaac aatcccaccg ggtgtgacgg tggagaacct
      541 gagcggcagt gacctgcggc atgcccgcag ggtgatgtaa cccgctgaca acggggattg
      601 aggcgagatc actaagccga gatgatcctc aaggttaagt actgaaaggc tgaagaacat
      661 gaacccgtta atccgcctct gtgggttgaa aacgtcacca cggcctacgt gatctgacag
      721 gccgtgcagg aggaactggc agtgatacgt aagcactacc ggtcgaaggt gttttgacat
      781 gtatgcgaaa caccggggca gcagcgtcta tcacgcttgc gttgctgact tctgccaact
      841 tgcggcaagc aaggataaag agtgcgacgg gcagcctcct cagtatgcct gagtccaggc
      901 aggtaaaccg gggaaggtca gcgacggatg ttaagggggc atggctccga tgacgcgctg
      961 gctggcggag cttccgtagt agtccgcgat ggggaaagcc cattacatgg cgaagggaag
     1021 cagtttgaat gtgtttgcga cgtgaattaa ctgacctaac gaggtgaaga cctttgataa
     1081 tcagcgaaat gcaacgcaag cttgccacat gggcagccac cgatccgtcc ctacggattc
     1141 aacggctgct gcgtctgata acacaaccag aatggctggc tgaagcggcg cggatcacgc
     1201 tttcatcaaa gggggcccat acccccggcg ttgatggcgt gaacaaaaca atgctacagg
     1261 ccagactggc tgttgagctg caaatcctca gggatgaatt actctcaggc cactaccagc
     1321 ccttgcccgc cagacgggtt tacatcccta aaagcaacgg caaactgcga ccactgggta
     1381 t
//
LOCUS       DAPOZO010000244         1373 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPOZO010000244 DAPOZO010000000
VERSION     DAPOZO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.249"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(17..811)
                     /locus_tag="ROI21_004853"
     CDS             complement(17..811)
                     /locus_tag="ROI21_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDX5113650.1"
                     /translation="MTLPTTIYSFPAYSPRFSSTDKPVKLKFHQYARATLLSNRGRDH
                     NCDGRHTVEIHKLDLSDWQAFNKLATRCNTYDGITMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQGRDIRCDHMHLTPANHPELWTQENGEWIYLRN"
     gene            1086..1373
                     /locus_tag="ROI21_004854"
                     /pseudo
     CDS             1086..1373
                     /locus_tag="ROI21_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT          401 a          246 c          297 g          429 t
ORIGIN      
        1 tatttttcgt caggtattag tttcgcaagt agatccattc accattctct tgtgtccata
       61 attcaggatg gtttgctggc gttagatgca tatgatcgca ccttatgtct cggccctgcg
      121 gcactcgctc gaaacccaaa cccgagtaga ttgctgctga acttgataag gaacgcagac
      181 caattttcaa atcatggggt aaatcagaat acgcgtttag caatgcagca atgatgaatc
      241 cgagggcatt agaaacgcct gtaacagtgc gaagttggtc gatataaaga tcgattgttg
      301 gtagttcaga tgaccagcaa ttaggcattt gggcaggaag ggttacaacg tatcctatcg
      361 tttggccggt atagtctgga gtaccataac ctggacccaa aacaacctcg tgtattgtgt
      421 atttactgtt gggttcgaag gattcgacat cttgcaataa atatttcagt tctgcggtta
      481 acctcgcacc cgcgtaagct ttattgtatt tctgggcgtg aatattatct aatgtagctt
      541 catggttcca tccaaaagaa ttatcaccat tcattgttat tccatcgtaa gtattacacc
      601 tcgtcgctaa cttattgaat gcttgccagt ctgagagatc tagtttgtgg atctcaacag
      661 tatgtctgcc atcgcaatta tggtctcttc cacgatttga aagtaatgta gcgcgtgcgt
      721 attgatgaaa ttttaatttt actggtttgt cggtggacga aaaacggggt gaatatgcag
      781 gaaatgaata aatagttgtt ggtaatgtca ttttattgtt gcctgtttaa tatttttaca
      841 aatatcgcag ttgaatgttc aaagaaaaag agttgatatt aataaaaata agtttgtttt
      901 ctgaagtggt gaaaatagcg gatagtaaaa gatattaata tcaaaaatcg ctcatcacgg
      961 atataatgat gcacccaggg agggagatga ctgacaacgc cttacaccat acattcttgt
     1021 tgtgcggtat gtaattattc tacgctgtcg gtatcggtgc gttcgttaat cagcaggtta
     1081 accagccaca aaaatccatt gaaaaaatca tcgattttac ctggggttat tatattgcta
     1141 atccctggtt tttaaaaatt gtgcatagtg aaaaccaaag taaaggtgtg cattatgcaa
     1201 aatcacagcg tctcttagaa attaatcatg cgcacttgca gttaatggaa tccttattgg
     1261 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgtatatta
     1321 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctg
//
LOCUS       DAPOZO010000245         1370 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPOZO010000245 DAPOZO010000000
VERSION     DAPOZO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.250"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            149..395
                     /locus_tag="ROI21_004855"
                     /pseudo
     CDS             149..395
                     /locus_tag="ROI21_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(522..716)
                     /locus_tag="ROI21_004856"
     CDS             complement(522..716)
                     /locus_tag="ROI21_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113651.1"
                     /translation="MVIRKKKCRECDNAITHNTVCCPYCGSVDLFGYYRNTDRIFTAL
                     LALLVVVLLVTSAVSMYVLW"
     gene            912..>1370
                     /locus_tag="ROI21_004857"
     CDS             912..>1370
                     /locus_tag="ROI21_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDX5113652.1"
                     /translation="MTYKYNPFWQQRIREMVLCALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARINAYQKRKRREGKRVHPTTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRDPGSLAGMIKQAWCSALGVDTGDHTTLVHFPVRPAVWLAR"
BASE COUNT          323 a          364 c          372 g          311 t
ORIGIN      
        1 gcacgggagt aatgagtttg gggagggtac cagaatgtca gtctggtgcg gtatgctggc
       61 agtatcacgt tgattacagc ataaagtaac gagaggctaa tagcggttaa aaccagagca
      121 gttccgtcgt tgttcatccc gggagacgct ggaaaaagtg atttcccgca cacgttataa
      181 ccttacccct gcggagccgg aagccttctg cggtcgatcg gctggcagaa ctgacgacaa
      241 attttacgcg tgtggatcca gtctggtcac ctgagtatcc ggtaatcaca acggtctggt
      301 gttatctgct ctgtgtgatt ctttttaggt cttctccggt tgttggtaac ctggataata
      361 ccgcgaacgt taagcggtat cgtgacaacg aataatttga aattaaaagg ggcagggtgg
      421 tctgcttcgg ggattaacgg cttacagcgt gtgggttgac ggcgcgttgc aggggacgaa
      481 ctctggcaac cctcacggat atctgataag ccccgttacc gctaccacag cacatacatg
      541 ctgacagcag atgtcaccag caaaaccaca accagcagcg ccagcagggc cgtgaagatc
      601 ctgtccgtat tccggtaata gccgaagagg tcgacagaac cacagtacgg acaacacacc
      661 gtattatgag taatcgcgtt atcacactcc ctgcacttct ttttgcgtat caccatcact
      721 ccctcctcac agccttaccc gtaaccggtt ttttacatta aaaccccctg aaaattcaac
      781 cgtcagtccg gagacaaccg ttcgggttat cacagagtgc ctgaggcatt gtcctgccgg
      841 aggtcacgtc agggactctg cgcatttttt acggcgtgga tattccgtat gaaccatacg
      901 gagatttaac catgacctac aaatacaacc ccttctggca acaacgcatc cgtgagatgg
      961 tgctgtgcgc actggatgtt catccccgcc tgacggcact tcgggttgac ctgcggctcc
     1021 cggatgtacc ggcagcaacg gacgcggcgg tgatatcccg ctttatcaat gccctgaaag
     1081 cccgaatcaa cgcttaccag aagcgtaagc gccgtgaagg taaacgcgtg catcccacga
     1141 ccctgcatta cgcctgggcc cgggagttcg gggagctaaa aggcaaaaaa cactatcacc
     1201 tgctgctgct ggtcaaccgg gatacctggt gtcgtgcagg tgattaccgc gatccggggt
     1261 cactggccgg gatgattaaa caggcctggt gcagtgccct gggtgtggat accggagatc
     1321 ataccacgct ggtgcatttt ccggtcaggc cagcggtgtg gctggcgcgt
//
LOCUS       DAPOZO010000246         1306 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPOZO010000246 DAPOZO010000000
VERSION     DAPOZO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.252"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            1..506
                     /locus_tag="ROI21_004858"
                     /pseudo
     CDS             1..506
                     /locus_tag="ROI21_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            515..>1306
                     /locus_tag="ROI21_004859"
     CDS             515..>1306
                     /locus_tag="ROI21_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDX5113653.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIG"
BASE COUNT          211 a          395 c          408 g          292 t
ORIGIN      
        1 acgttctgat ccggcgtgcc cggcaaggca gcggcctcgt gacggatctg gtgcccggcc
       61 agtccggtgg aggtaaaggt aaggggcgct tgccggaacc ggtcgagcgc gtcatccacg
      121 agctactgca aaagcggttc ctgaccaagc agaagcgcag cctagcggcc tttcaccgcg
      181 aagtcactca ggtgtgcaag gctcaaaaac tgcgagtgcc ggcgcgcaat accgtggcct
      241 tacggatcgc tagccttgac ccgcgcaagg tcatccgccg gcgggaaggc caggatgccg
      301 ctcgtgacct acaaggtgtg ggcggcgagc ctcctgccgt gaccgcgccg ctggagcagg
      361 tgcagataga ccatacggtc atcgacctga tcgtggtcga tgaccgcgac cggcaaccta
      421 ttggccgccc gtacctgacc ctcgccatcg acgtgttcac ccgctgcgtg ctcggcatgg
      481 tcgtcacgct ggaagcgccg tctgccccga tttattgttc acagcgatag cacctgccat
      541 ttggggcagc acctacattg tcaccaccca atacctgccg aacttctcac cgatgacggt
      601 cgcgatgctg cgggcgttgc cggcgggttt attgctcgtg atgatcgtcc gacagattcc
      661 aacgggaatc tggtggatgc gcatcttcat cctcggcgca cttaatattt cgctattctg
      721 gagcttgttg tttatttcgg tctaccgcct gccgggcggg gtcgcggcga cggtaggcgc
      781 tgtgcagccg ctgatggtcg tgttcatctc tgccgctctg ctaggtagcc cgatacgatt
      841 gatggcggtc ctgggggcta tttgcggaac tgcgggcgtg gcgctgttgg tgttgacacc
      901 aaacgcagcg ctagatcctg tcggcgtcgc agcgggcctg gcgggggcgg tttccatggc
      961 gttcggaacc gtgctgaccc gcaagtggca acctcccgtg cctctgctca cctttaccgc
     1021 ctggcaactg gcggccggag gacttctgct cgttccagta gctttagtgt ttgatccgcc
     1081 aatcccgatg cctacaggaa ccaatgttct cggcctggcg tggctcggcc tgatcggagc
     1141 gggtttaacc tacttccttt ggttccgggg gatctcgcga ctcgaaccta cagttgtttc
     1201 cttactgggc tttctcagcc cggggaccgc cgtgttgcta ggatggttgt tcttggatca
     1261 gacgctgagt gcgcttcaaa tcatcggcgt cctgctcgtg atcggg
//
LOCUS       DAPOZO010000247         1300 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPOZO010000247 DAPOZO010000000
VERSION     DAPOZO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.253"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..286
                     /locus_tag="ROI21_004860"
     CDS             <1..286
                     /locus_tag="ROI21_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113654.1"
                     /translation="KDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNAL
                     LNILESQFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(608..1210)
                     /locus_tag="ROI21_004861"
     CDS             complement(608..1210)
                     /locus_tag="ROI21_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113655.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLSYRTTVDDKRT"
BASE COUNT          308 a          324 c          347 g          321 t
ORIGIN      
        1 gaaggacgcc gaggagctgt tcgactggct cattgaggag tgccgcggag aatgcccgcc
       61 ccgtgtcagg aaaaattata ttctccagtg tggcccgggc cggttcagaa acaggaaaaa
      121 actgaacgca ttgctgaaca ttcttgaatc tcagttcagg ctttctgtcg tgccggaagg
      181 aaaaaccatg tatgttctgc ttccccagat agccagttta aagttgtctg atgtctctgg
      241 tatttttacc tcgggttatc actacaataa gctgagagcg aagtaaccgg tgagcagttt
      301 ttctccatta tgcttcagac ttagatgatt gattattatg gggatttcat caccgatatt
      361 cagatgtatg aatatcggtg atgaactaaa gctcaggagc tgcgtggtgg tgatgaatca
      421 acaggcatat aactgatacc cgccggtcag aagttccagc ctgcgacttt cctgtctgcg
      481 tgcccgcagt ccggcgttca ggtgcagcgt gtgacagaac gcacggagag cgaaacggca
      541 ggcagacagg ccgttcagtc agccctggca ccctgcccgg ataccagctt ccctgaaaca
      601 actggcatca ggtgcgctta tcgtcaaccg ttgtgcggta tgacaggagc acgtactgat
      661 ccatcagatc cgtcagtgct gcgctctgct tctgacggtt gatgatccat tcctccagct
      721 ctttttctga tttccgtttc agattctccg tccagtcgtc aaggcagttt ccggtcatta
      781 aatcaagcca gcaaagccag accagtttga ggcgcaactc cgtgccacca aactgacgga
      841 agaggctgat cagtttattt gccagagaac gctgcctcag acgttcacat tcagacagga
      901 gaatgccttc cagcatcgga tgatgaacca gcagatgttc actggtgcat aaattcacta
      961 tcagatcatc atccaggggc ggtgtggccc gtttgtcagg gccataaatc agccggtgca
     1021 ggctctgggg tacgatggca atctccggtt tttcaccatt gaatgacacc caaccgggcg
     1081 tcagattatg gaagtagtag atattcggat gacacacgcc ccaggatacg gcgtgccggg
     1141 tgaagccggt cagccatttc tggtagcttt cgtggagcca ttcgtagcgg gtctgatact
     1201 gttgtttcat tttcatattc ctcacgggga tataacaggc agcagaatgc cgccggcagg
     1261 ccggagatta cgtctgctgc gggtgaacgg ataacggtca
//
LOCUS       DAPOZO010000248         1265 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPOZO010000248 DAPOZO010000000
VERSION     DAPOZO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.254"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(279..677)
                     /locus_tag="ROI21_004862"
     CDS             complement(279..677)
                     /locus_tag="ROI21_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113656.1"
                     /translation="MEMLGKILVIIISMIIISCDYYNSGMDYQDNIALEQVVTWDSDN
                     IFTPNIFEHNGRCFMKKNETWMLLNGDATPNIRTMSNKKIECLEKSEVEWASRVCDSI
                     TDKERENLKALICSKPAKSVNTGKRPALGY"
     gene            complement(678..>1265)
                     /locus_tag="ROI21_004863"
     CDS             complement(678..>1265)
                     /locus_tag="ROI21_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HDX5113657.1"
                     /translation="GLYYNRHRYYDPLQGRYIIQDPIGGDGGWNLYQYPLNPVQHVDP
                     LGLSTMIIGNGPVPDNPFGHAAAANRYGLMSSGTGDEMGASVSDYFKKMQPRRDTWIW
                     IIDTTDEEEQCMMNKAIDLNKKLKTINLGPLPVANNCFSRTNMIFEACGFNNPSMNTN
                     APISLQVLGEFYGYQRYIMPKGPFTGFPFKFQEVK"
BASE COUNT          343 a          277 c          226 g          419 t
ORIGIN      
        1 atttgatcat gtataagcaa taaaaaacag ctttaggtaa tgaggaatat ctcaattttt
       61 aaacataaaa tgcgaattat ttagtacaaa aaagcagcgt tacgtgatct ttccctgccc
      121 tatcgggtct gtgtgatata tcgtccctgt cccggattgt agtaccggtg ccagttgtag
      181 tgcagttcgg attcgtcgtc atactgctag cccggatggc ggagcagttg ctctagtcac
      241 tcagaatatt gtaatagtaa tatagtgcag tgccggtttc aatatcctaa cgctggtctt
      301 ttgcctgtat taactgattt agctggtttg ctacatatta atgccttcag attttccctt
      361 tccttgtctg tgatactgtc gcaaacccta ctagcccatt ctacttcact cttctccagg
      421 cattcaattt tcttattgct cattgttcta atgtttggtg ttgcatctcc atttaataac
      481 atccatgttt catttttctt cataaaacat cttccattat gttcaaagat attaggtgtg
      541 aatatattat cagaatccca tgttacgact tgctcgaggg ctatattatc ttgatagtcc
      601 atgccagaat tataataatc acaagatata attatcatgg aaataattat cactaatatc
      661 ttcccaagca tttccattta ctttacctct tgaaatttaa atgggaatcc agtgaaagga
      721 cctttaggca taatatatct ttgatatcca taaaactcac cgagcacttg taggcttata
      781 ggcgcatttg tattcataga ggggttgtta aacccgcacg cctcaaaaat catgttcgtt
      841 cggctaaaac aattatttgc aacaggaaga ggcccgaggt taatagtttt tagtttttta
      901 ttaaggtcta ttgccttatt catcatacat tgttcttctt catctgttgt atctattatc
      961 caaatccagg tatctctgcg gggttgcatc tttttaaaat aatcagagac agaggcgccc
     1021 atctcatccc cagttcccga actcattaat ccatatcgat ttgccgccgc agcatgccca
     1081 aaaggattat caggaacggg gccattacct attatcatag tactaaggcc taatggatca
     1141 acgtgttgca ctggattcag cggatattgg tacagattcc atcccccatc cccccctatc
     1201 gggtcctgga tgatataccg cccctgcaac ggatcgtagt accggtgacg gttataatac
     1261 agccc
//
LOCUS       DAPOZO010000249         1258 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPOZO010000249 DAPOZO010000000
VERSION     DAPOZO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.255"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..436
                     /gene="istA"
                     /locus_tag="ROI21_004864"
     CDS             <1..436
                     /gene="istA"
                     /locus_tag="ROI21_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HDX5113658.1"
                     /translation="YQTGQHRFHPSLWQFGKEMGFSPRLCRPFRAQTKGKVERMVQYT
                     RNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVANQRKHETIQARPCDRWLEEQQS
                     MLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDSFCRGVA"
     gene            436..1215
                     /gene="istB"
                     /locus_tag="ROI21_004865"
     CDS             436..1215
                     /gene="istB"
                     /locus_tag="ROI21_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HDX5113659.1"
                     /translation="MMELQHQRLMVLAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          342 a          328 c          323 g          265 t
ORIGIN      
        1 atatcagacc ggtcagcacc ggttccatcc ttcgctgtgg cagttcggca aggagatggg
       61 cttctctccc cgactgtgtc gccccttcag ggcacagact aaaggtaagg tggaacggat
      121 ggtgcagtac acccgtaaca gtttttacat cccactaatg actcgcctgc gcccgatggg
      181 gatcactgtc gatgttgaaa cagccaaccg ccacggtctg cgctggctgc acgatgtcgc
      241 taaccaacga aagcatgaaa caatccaggc acgtccctgc gatcgctggc tcgaagagca
      301 gcagtccatg ctggcactgc ctccggagaa aaaagagtat gacgtgcatc ttgatgaaaa
      361 tctggtgaac ttcgacaaac accccctgca tcatccactc tccatctacg actcattctg
      421 cagaggagtg gcgtgatgat ggaactgcaa catcaacgac tgatggtgct cgccgggcag
      481 ttgcaactgg aaagccttat aagcgcagcg cctgcgctgt cacaacaggc agtagaccag
      541 gaatggagtt atatggactt cctggagcat ctgcttcatg aagaaaaact ggcacgtcat
      601 caacgtaaac aggcgatgta tacccgaatg gcagccttcc cggcggtgaa aacgttcgaa
      661 gagtatgact tcacattcgc caccggagca ccgcagaagc aactccagtc gttacgctca
      721 ctcagcttca tagaacgtaa tgaaaatatc gtattactgg ggccatcagg tgtggggaaa
      781 acccatctgg caatagcgat gggctatgaa gcagtccgtg caggtatcaa agttcgcttc
      841 acaacagcag cagatctgtt acttcagtta tctacggcac aacgtcaggg ccgttataaa
      901 acgacgcttc agcgtggagt aatggccccc cgcctgctca tcattgatga aataggctat
      961 ctgccgttca gtcaggaaga agcaaagctg ttcttccagg tcatcgctaa acgttacgaa
     1021 aagagcgcaa tgatcctgac atccaatctg ccgttcgggc agtgggatca aacgttcgcc
     1081 ggtgatgcag cactgacctc agcgatgctg gaccgtatct tacaccactc acatgtcgtt
     1141 caaatcaaag gagaaagcta tcgactcaga cagaaacgaa aggccggggt tatagcagaa
     1201 gctaatcctg agtaaaacgg tggatcaata ttgggccgtt ggtggagata taagtgga
//
LOCUS       DAPOZO010000250         1219 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPOZO010000250 DAPOZO010000000
VERSION     DAPOZO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.256"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(97..498)
                     /locus_tag="ROI21_004866"
     CDS             complement(97..498)
                     /locus_tag="ROI21_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113660.1"
                     /translation="MHSLNDDFYVLTGKIFCRLLNTHPAPQVLIYSGFFDIPDQVDHD
                     QKKCRCFIDTLIWLRDNSYIRYSQLLAVGAADVVLTEKTLCLLNAMPTFLQPRPVPFK
                     ESLLDAVKTGENAVIAECVSRIMRYVFNERK"
     gene            500..715
                     /locus_tag="ROI21_004867"
     CDS             500..715
                     /locus_tag="ROI21_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113661.1"
                     /translation="MNPRLTLTEHQRRAEAVNNVLEDIIRLYRGELSVCRAAFHFQGI
                     QKQFDTSVFAEGITYALDRIRSENRPG"
     gene            906..1219
                     /locus_tag="ROI21_004868"
                     /pseudo
     CDS             906..1219
                     /locus_tag="ROI21_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664222.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
BASE COUNT          338 a          296 c          297 g          288 t
ORIGIN      
        1 ggcgagagga gaatgggcat cgctgtttga aagagtttcc ccggaattac ttcccctcgc
       61 actggctatc gggattatgc agacagcgga taaacctcac ttacgttcat taaacacgta
      121 acgcataatc cgggatacgc attcagcgat gactgcattt tcaccggttt ttactgcatc
      181 cagaagtgac tctttaaatg gtacgggccg gggctgcaga aaggtcggca tggcattgag
      241 cagacagagc gttttttccg tgaggacgac atcagccgct cccacagcca gcaactgaga
      301 gtaacgaata tagctgttat cccgaagcca tatcagcgta tcaataaaac accggcactt
      361 tttctggtcg tgatcaacct ggtccgggat atcgaaaaaa ccagaataaa tcaaaacctg
      421 aggtgccgga tgcgtgttca gtaatcggca gaatattttg ccggtgagta cataaaaatc
      481 atcatttaag gagtgcatca tgaatccccg attaacgtta acagaacatc agcgccgtgc
      541 tgaagcagtg aacaatgttc tggaagatat tatccggtta taccgcggag aactgagtgt
      601 ctgtcgtgcc gcttttcatt ttcagggaat acaaaagcag tttgatactt ctgtttttgc
      661 agaaggtatt acttatgccc tggaccggat aaggtcagaa aatcgccccg gataatccct
      721 aaaccacatt aacagaaatc ccgccatccc tgccgcatca tgaaagaacg accagcccgg
      781 cacccgaaag ccgggctggt catcgcccca caggaatgcg ctgtgagccg accaaggttc
      841 agtcagtctc gcgagacctg accagactgc catatttttt aataattgta aaaggcttgc
      901 agattatgaa aatgtagaca ttacccatcg tgccgtggat tggtggcaaa cgccgccttg
      961 caaaacacat tctgccgctg tttccggcgc atgaatgtta tgtggagccg ttttgtgggg
     1021 cagcagcgct ctattttctt aagacacccg gcaagatcga agtcatcaat gatatcaacg
     1081 gtgaactggt gaatctgtac cgggtggtaa agcatcacct ggaagaattt gtccgccagt
     1141 tcaaatgggc gctggtcagc cgtcagatct acaaatggct gcagatcacc ccggaagaaa
     1201 cactgacgga tatccagcg
//
LOCUS       DAPOZO010000251         1211 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPOZO010000251 DAPOZO010000000
VERSION     DAPOZO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.257"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..113)
                     /locus_tag="ROI21_004869"
     CDS             complement(<1..113)
                     /locus_tag="ROI21_004869"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDX5113662.1"
                     /translation="MKNEPLISILIPTYNVGAWIDEAINSIIEQTYKKIEI"
     gene            complement(110..>1211)
                     /gene="wzy"
                     /locus_tag="ROI21_004870"
     CDS             complement(110..>1211)
                     /gene="wzy"
                     /locus_tag="ROI21_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021523152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O102 family O-antigen polymerase"
                     /protein_id="HDX5113663.1"
                     /translation="SGKYLTAFLCCAFLSINSLAINPAYIYGAGLLSIIIMLIFVKAK
                     ISKFSMVTIIFAFAILLCQVIGIYYKTQTTGYPEAPNYITPFLFFYSMVISVGVNEVF
                     MVLNYDTRIKCYKNFLMFFVMFMFLELITRVIIGNLSEGILYAFKKSLFYFDSNFTAL
                     IDISILGFLLFLKNNKIYNFKYTRYITYILLFLTMSRAAMVTLLAMLYVLNNKAKIGK
                     RSALVFLFVICVFIYMSISYLFENSNYSNIDGSFNSKFYIISQAFEFYTTQSLMTHMF
                     GVGLGNTEQLLGIFAHNIYVTLVLEFGFIGSFLFITFIFYSLKKTNFNSLYIWLPVCI
                     SGISLFGAYSPFLFILNAVIYNETRYLGNQKL"
BASE COUNT          481 a          184 c          153 g          393 t
ORIGIN      
        1 attatttcta tttttttata agtttgttca ataatactat ttatggcttc atcaatccat
       61 gctccgacat tatacgtcgg aattaatatt gaaattaatg gttcattttt cataactttt
      121 gattacctaa atatctggtt tcgttataaa taacagcatt caatataaat aaaaatggtg
      181 aatatgcacc aaatagcgaa ataccactga tacatactgg caaccaaata tacaatgaat
      241 taaaatttgt ctttttaagg ctataaaaaa taaaagttat aaaaagaaaa gacccaataa
      301 aaccaaattc taaaactaaa gttacatata tgttatgtgc aaaaatccct agtaattgtt
      361 ctgtgtttcc aagcccaaca ccaaacatat gtgtcataag tgattgcgta gtgtaaaatt
      421 caaatgcttg gctaataatg tagaatttag aattaaaact cccatcaata tttgaataat
      481 tgctattttc aaataagtag cttattgaca tgtaaataaa tacacatatg acaaataaaa
      541 aaacaagtgc tgaacgcttt cctatctttg ccttattatt cagaacatat aacatagcaa
      601 gcagcgttac cattgcagct cttgacattg ttaaaaatag cagaatatat gttatgtacc
      661 ttgtatattt gaagttatag attttattat ttttaagaaa caataaaaaa cctaagatgg
      721 aaatatctat taatgctgta aaatttgaat caaagtagaa taaacttttt ttgaatgcat
      781 ataaaatacc ttctgataga ttacctataa tgactctagt aatcaactcc agaaacatga
      841 acattacaaa aaacatcaaa aaatttttat aacatttaat tcgtgtatca tagtttagca
      901 ccataaatac ttcatttact ccaacgctaa taaccatcga gtaaaaaaac aggaagggtg
      961 tgatgtaatt tggagcttca ggataccctg ttgtttgggt tttataatag atacctataa
     1021 cctgacacag aagaattgcg aaagcaaaaa taatagttac catgctaaac tttgaaattt
     1081 ttgccttaac aaatattagc attattataa tacttaacaa ccctgcacca tatatataag
     1141 caggattaat agcaagagaa tttatgctca aaaatgcaca acataaaaat gccgtaagat
     1201 acttaccgga t
//
LOCUS       DAPOZO010000252         1205 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPOZO010000252 DAPOZO010000000
VERSION     DAPOZO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.258"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            75..347
                     /locus_tag="ROI21_004871"
     CDS             75..347
                     /locus_tag="ROI21_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113664.1"
                     /translation="MITPQEARQRTRTLVEHYVNECECRDLTNVKHVLTALISMATQA
                     IVATNGKEAALQVLMNTLTHTAEHEVPYRMETTAEGGLHITVSRKH"
     gene            357..653
                     /locus_tag="ROI21_004872"
     CDS             357..653
                     /locus_tag="ROI21_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113665.1"
                     /translation="MTRNTELTRTALYRLALQRFGPDAQALKLAEEAAELAASAARNL
                     NGQGSESDLAAELADVEIMTEQLRLQGMDRLIDFHKQKKLERLAARLGVIYTNE"
     gene            668..883
                     /locus_tag="ROI21_004873"
     CDS             668..883
                     /locus_tag="ROI21_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113666.1"
                     /translation="MADILREITAWTLILTGLATCLSAGAALAALLMHITTQWLWEKL
                     KAAYSLKELSDAVRAWKRQKNTGDTEQ"
     gene            880..>1205
                     /locus_tag="ROI21_004874"
     CDS             880..>1205
                     /locus_tag="ROI21_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HDX5113667.1"
                     /translation="MTDQNKHIEKLKKLLALAASGNPHEAALALRRARKLMDVHGITH
                     SDIAMSDIDETISHYWPTGSLRPPRYMLGLMNIIREVFGVNSIIHPGTHPSVGFYGNR
                     ERAALAA"
BASE COUNT          311 a          358 c          336 g          200 t
ORIGIN      
        1 ccgtactgca gccagcattt ttcggcagac gtgcccgtca tggaattctc ccgccctgac
       61 agggaggact cgcaatgatt accccacagg aagcacgaca gcgcacccga acccttgttg
      121 aacactatgt caacgagtgt gaatgccgcg acctcaccaa tgtgaagcac gtcctgacgg
      181 cgctaatcag catggccaca caggccattg tggcgaccaa cggaaaggag gctgccctgc
      241 aggtactgat gaacacactc acccacacgg cagagcatga ggtgccgtac cggatggaaa
      301 ccactgcaga aggcggcctg cacatcaccg tcagccggaa gcactgaggg cgcggcatga
      361 cacgaaacac cgaactcacc cgcaccgccc tctaccgtct ggccctgcag cgtttcgggc
      421 cggacgcaca ggccctgaaa ctggcagaag aggccgctga actggcggcc agtgccgccc
      481 gcaacctgaa cggacagggc agcgaaagtg acctcgcggc agagctggca gacgtggaaa
      541 tcatgacaga gcaactgcgc cttcagggga tggaccggct gattgacttc cacaaacaga
      601 aaaaactgga acgtctggct gcacgactgg gcgtgattta cacgaacgag taaccgggag
      661 gcattcaatg gctgacatac tcagggaaat caccgcatgg acactgattc ttactggcct
      721 ggcgacatgc ctcagtgcgg gggcagccct ggctgccctg ctgatgcaca taacaacaca
      781 gtggttatgg gaaaagctta aagcagcata cagcctgaaa gagctgtccg acgctgtccg
      841 ggcatggaaa cggcagaaaa ataccggaga tacagaacaa tgacagacca gaataaacac
      901 attgagaaac tgaaaaagtt gctggcgctg gccgcatccg gcaacccgca cgaggccgct
      961 ctggcactgc gccgagcccg taaactgatg gatgttcacg gcatcacaca ttccgacatt
     1021 gctatgagtg atattgatga aaccatcagt cactactggc cgacaggcag tctccgtcca
     1081 ccgcgctaca tgctgggcct gatgaacatc atccgcgagg tatttggtgt taactccatc
     1141 attcaccccg gcacgcatcc gtctgtgggg ttctacggca accgggaacg tgcggcactg
     1201 gctgc
//
LOCUS       DAPOZO010000253         1157 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPOZO010000253 DAPOZO010000000
VERSION     DAPOZO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.259"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>1157)
                     /locus_tag="ROI21_004875"
     CDS             complement(<1..>1157)
                     /locus_tag="ROI21_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDX5113668.1"
                     /translation="NALAGQTVSVTAGNGATVSPTVITGPDGTVEISVTSQTAGVSAV
                     TATINNSTASQNVMFIADVRTAKIADLVVIKDDSVADGAMANMLRARVTDAFGNALAG
                     QTVSVMAGNGATTAPTVTTQPDGTVEISATSQTAGISTVTATINNSSLSRNVMFVADV
                     RTAQIADLVVIKDGSVADGSTANMLRVRVTDAFGNALGGQTVSVLADNGVTTAPTVIT
                     EPDGTVEISVTSQTAGVSAVTATINSSSQSQNVTFIADVSTAKIADLVVIKDGSEADG
                     STANTLRVRVTDAFGNTLAGQTVSVLADNGATTAPTVITEPDGTVEISVTSQTAGVSA
                     VTASINSSSQSRNVTFVADVRTAQIADLVVIKDGSEADGATANTLRARVTDA"
BASE COUNT          182 a          406 c          309 g          260 t
ORIGIN      
        1 gcatcggtca ccctcgcccg cagcgtgttc gccgtcgcac cgtccgcctc tgaaccgtcc
       61 ttaataacca ccagatccgc gatctgcgcc gtcctgacat cggcgacaaa cgtcacgttc
      121 cggctctggc tgctgctgtt gatgctggca gtgactgcac tgactcccgc cgtctggctg
      181 gtgactgaga tctccaccgt accgtccggc tctgtgatga ccgtcggggc agtcgttgcg
      241 ccgttgtccg ccaacacgct gaccgtctgc ccggctagcg tgttcccgaa cgcatcggtc
      301 accctcaccc gcagcgtgtt cgccgtcgaa ccgtccgcct ctgaaccatc cttaatgacc
      361 accagatcag cgatcttcgc cgtgctgaca tcggcgataa atgtcacgtt ctgactctga
      421 ctactgctgt tgatggttgc agtgactgca ctgactcccg ccgtctggct ggtgacactg
      481 atttccaccg tgccgtccgg ctcggtgatc accgttgggg cagtcgttac gccgttgtcc
      541 gccaacacgc tgaccgtctg gccgccaagc gcattaccga acgcatccgt caccctcacc
      601 cgcagcatgt tcgccgtcga accgtctgcc actgagccgt ccttaatgac caccagatca
      661 gcgatctgcg ccgtcctgac atccgcgaca aacatcacgt tccggctcag gctgctgttg
      721 ttgatggttg cagtgactgt actgatcccc gccgtctggc tggtggcaga gatctccacc
      781 gtgccgtccg gctgcgtggt gaccgtcgga gcagtcgtgg caccgttgcc tgccatcacg
      841 ctgaccgtct gtccggcaag cgcattaccg aacgcatcgg tgaccctcgc ccgcagcata
      901 ttcgccattg caccgtctgc cactgagtcg tccttaatga ccaccagatc cgcgatcttc
      961 gccgtcctga catcggcgat aaacatcacg ttctggctcg cggtgctgtt gttgatggtt
     1021 gcagtgactg cactgactcc cgccgtctgg ctggtgacac tgatttccac cgtgccgtcc
     1081 ggccctgtga tgaccgtcgg agacaccgtt gcgccgttgc ctgccgtcac gctgaccgtc
     1141 tgcccggcaa gtgcatt
//
LOCUS       DAPOZO010000254         1073 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPOZO010000254 DAPOZO010000000
VERSION     DAPOZO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.261"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(147..1007)
                     /locus_tag="ROI21_004876"
     CDS             complement(147..1007)
                     /locus_tag="ROI21_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HDX5113669.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          285 a          254 c          240 g          294 t
ORIGIN      
        1 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
       61 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
      181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
      421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
      481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
      541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
      601 agtaagttgg cagcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
      661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
      721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgca
      781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
      841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
      901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
      961 gccgcaaaaa agggaataag ggcgacacga aaatgttgaa tactcatact cttccttttt
     1021 caatattatt gaagcattta ccagggttat tgtctcatga gcggatacat att
//
LOCUS       DAPOZO010000255         1016 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPOZO010000255 DAPOZO010000000
VERSION     DAPOZO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.262"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>1016)
                     /locus_tag="ROI21_004877"
     CDS             complement(<1..>1016)
                     /locus_tag="ROI21_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDX5113670.1"
                     /translation="QEFQDLPDGAETTLTNQHGADNWKILPGVPLYDTIVIVTGESVR
                     RDYMSVYGYPVPTTPWLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVV
                     ALAAKAGYSTWWISNQGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQET
                     ERALADKSSPKVIFLHMIGSHPNPCDRLNSWPNHYLGQYPRKIACYLASISKLDNFLG
                     QLDGILRRHSRHFAMLYFSDYGMSVSDSANPVHHDGHIQGGYSVPLIITASDITSHQS
                     VSRKISARHFAGIFQWLTGIRTENIPPFNLLTDEDNEPVMVFNGERNVLADSLKPQPL
                     ILPV"
BASE COUNT          262 a          248 c          256 g          250 t
ORIGIN      
        1 tgaccggaag aatcagaggc tgtggtttca gactgtctgc cagtacattc ctctctccgt
       61 taaaaaccat aacgggttca ttatcttcgt ctgtcagcag attgaatggc ggtatatttt
      121 cagtacgaat gccggtcagc cactgaaaaa tacctgcgaa atgacgggca ctgatttttc
      181 tgctgacgga ctgatgagac gtgatgtcac tggcggtaat aatcagggga acgctgtagc
      241 ctccttgtat atggccatca tgatgaacag gattagcact atcgctgacc gacatcccat
      301 agtcagaaaa gtaaagcatg gcgaaatgac gggaatgccg gcgaaggata ccatcaagct
      361 gaccgagaaa gttatccagt ttactgatgc tggcgaggta acaggcaatt tttcggggat
      421 actgccccag gtaatgattc ggccaggagt taagccggtc acacggattc ggatgagagc
      481 caatcatgtg caggaaaatc accttcggag aggatttatc cgccagtgca cgttctgttt
      541 cctgtaacaa caacatgtca tccgttttac gggaagcgaa gctgcctttc ttgaggaaaa
      601 cggtatgctc cgcatcagaa gcaataacag agatgcgtgt gtcatgctct cccagttttc
      661 cctgattgga tatccaccat gtgctgtatc ctgcttttgc tgccagcgcc accacgttgt
      721 tgccggaatc agggttctgc tcatagtcat aaatcagtgt ccggctcagg gaaggcacag
      781 tactggctgc tgccgatgta tagccgtcaa taaataaacc gggagcagta ttcagccacg
      841 gtgtggttgg cacgggatag ccatataccg acatataatc cctgcgcaca ctctcaccag
      901 tgacgataac aatcgtgtca tacaacggta cacccggcag gattttccag ttgtcagccc
      961 cgtgctgatt cgtaagcgtc gtctcagcac cgtctggcag atcctgaaat tcctga
//
LOCUS       DAPOZO010000256          993 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPOZO010000256 DAPOZO010000000
VERSION     DAPOZO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.263"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            74..298
                     /gene="hha"
                     /locus_tag="ROI21_004878"
     CDS             74..298
                     /gene="hha"
                     /locus_tag="ROI21_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002208622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDX5113671.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(630..827)
                     /locus_tag="ROI21_004879"
     CDS             complement(630..827)
                     /locus_tag="ROI21_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113672.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
BASE COUNT          259 a          249 c          255 g          230 t
ORIGIN      
        1 gcacgggagt aatgagtttg gggagggtac cagaatgcca gtcaggtgcg gtatgctggc
       61 agtatcacgt tgattgcagc ataaagtaac gagagacgaa tggaagttaa aaccagagca
      121 gttccgtcgt tgttcatccc gggagacgct ggaaaaagtg attacccgca cacgttataa
      181 acttacccct gcggagctgg aagcctttaa ctctgcggtc gatcaccggc tggcagaact
      241 gacaatgaac aaactttacg atcgcgtgcc ggcttccgtc tggaaatatg tcacctgaat
      301 cttgaatccg cgctacgatg actctggtgt tatctgtgca ccggaagatc gggaacatta
      361 agcagtataa cggcaacaaa gaattcgaaa tcaaaaggga atggtgtaca gaggggcagg
      421 cgttcaggca ttaccggcct gtatcttctg aaggggactc gtatttgtcg gggctgagta
      481 ttccgctcga ttcgatcacg gattggcaga aacctgactg tgattgattc aggtaccatt
      541 ttgcagggat ccggtgcgtt actgtctggc acaacaaacg cagcgggtcg ctctgccccg
      601 ccgactcctg ataatccctc ccctgtaacc tactgcaaca gtacaaatac actgacgcct
      661 gaaacggtaa ccagaatcag caccagcagt aacgtcagta aacagagcag acgatcggtc
      721 ttccggtaat aaccgaaggg gtcgacagca ccacaatacg ggcaacatac tgtattatga
      781 gtaattgcgt taccacagtc cctgcacttc tttttgcgta tcacgatcgc tccctctcca
      841 cgccttatcc gtaacctgtt ttttacatta aaaaaacaac gccgggaatt caatcgtcag
      901 tccggcgatg accgttcggg ttatcacaga gtgcctgaga cagtgtcctg ccggaggtca
      961 cctcaggggc tctgcgcatt ttttacgacg tgg
//
LOCUS       DAPOZO010000257          987 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPOZO010000257 DAPOZO010000000
VERSION     DAPOZO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.264"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            1..987
                     /locus_tag="ROI21_004880"
                     /pseudo
     CDS             1..987
                     /locus_tag="ROI21_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          280 a          234 c          261 g          212 t
ORIGIN      
        1 aggcacttta ggacaatggg ttaccgccgc cagaaaaggg ctcggtactc ctggttcccg
       61 cacggtggct gaactggaat ctgaaattct gcaactgcgt aaggcgttaa atgaagctcg
      121 ccttgagcga gatatattaa aaaaagcaac tgtagatttt gcacaggagt cgctgaaaaa
      181 tacgcgttaa tcgaacaatg gcgacaacaa tttcccattg aagcgatgtg tcaggtattt
      241 ggtgtatcca ggagcggtta ttacaactgg gtacagcatg aaccctcaga cagaaaacaa
      301 agtgatgagc ggctaaaact ggagattaag gtggcacata tccgcactcg cgaaacatat
      361 ggaacccggc ggctccagac ggagctggca gagaatggca tcatcgttgg tcgtgaccga
      421 ctggcacgtc ttcgtaagga gctaaggcta cgctgtaagc agaaacgcaa gttcagagcg
      481 actacgaact cgaaccacaa tctgccagtt gcgccaaatc tgctgaacca gacgttcgct
      541 cctacagcac caaatcaggt ctgggtggcg gacctgacgt atgttgccac acaggaggga
      601 tggttgtacc tcgctggcat caaagatgtt tatacgtgcg aaattgtcgg ctacgccatg
      661 ggagagcgca tgacaaaaga gctgacaggt aaagccctgt ttatggcgct caggagccag
      721 cgcccacctg ccgggctaat ccaccactct gatcgaggtt cacagtactg cgcatacgat
      781 taccgggtca tacaggagca gtttggtctg aaaacatcaa tgtcgcgtaa aggtaactgt
      841 tacgacaacg ctccgatgga aagcttctgg ggaacgctga aaaatgagag cctgagccac
      901 tatcgtttta ataaccggga tgaagccatc tcagtaatac gggaatacat tgagattttc
      961 tacaatcgtc agcgtcgtca ctctcgt
//
LOCUS       DAPOZO010000258          984 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.265, whole genome shotgun sequence.
ACCESSION   DAPOZO010000258 DAPOZO010000000
VERSION     DAPOZO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.265"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            75..347
                     /locus_tag="ROI21_004881"
     CDS             75..347
                     /locus_tag="ROI21_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113673.1"
                     /translation="MITPQEARQRTRTLVEHYVNECECRDLTDVKHVLTALISMAAQA
                     IVATNGKAAALQVLMNTLTHTAEHEVPYRVETTAEGGLNITVDRKH"
     gene            357..662
                     /locus_tag="ROI21_004882"
     CDS             357..662
                     /locus_tag="ROI21_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113674.1"
                     /translation="MTRNTELTRTALYRLALQRFGPDAQALKLTEEAAELAASAARNL
                     NGQGSESDLAAELADVEIMTEQLRLQGMDRLIDFHKQKKLERLAARLGVMYTGDTEQ"
     gene            659..>984
                     /locus_tag="ROI21_004883"
     CDS             659..>984
                     /locus_tag="ROI21_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HDX5113675.1"
                     /translation="MTDQDKHIEKLKKLLALAASGNPHEAALALRRARKLMDVHGITH
                     SDIAMSDIDETISHYWPTGSLRPPRYMLGLMNIIREAFGVNSIIHPGTYPGVGFYGNR
                     ERAALAA"
BASE COUNT          240 a          308 c          278 g          158 t
ORIGIN      
        1 ccgtactgca gccagcattt ttcggcggac gtgcccgtca tggaattctc ccgccctgac
       61 agggaggacg cgcaatgatt accccacagg aagcacgaca gcgcacccga acccttgttg
      121 aacactatgt caacgagtgt gaatgccgcg acctcaccga tgtgaagcac gtcctgacgg
      181 cgttaatcag catggccgct caggccattg tggcaaccaa cggaaaggcg gctgccctgc
      241 aggtactgat gaacacactc acccacacgg cagagcacga ggtgccgtac cgggtggaaa
      301 ccactgcaga aggcggcctg aacatcaccg tcgaccggaa acactgaggg cgcggcatga
      361 cacggaacac cgaactcacc cgcaccgccc tctaccgtct ggccctgcag cgtttcggtc
      421 cggatgcaca ggccctgaaa ctgacagaag aggccgctga actggcggcc agtgctgccc
      481 gcaacctgaa cggacagggc agcgaaagtg acctcgcggc agagctggca gacgtggaaa
      541 tcatgacaga gcagctgcgc cttcagggga tggaccggct gattgacttc cacaaacaga
      601 aaaaactgga acgccttgcc gcacggctgg gcgtcatgta caccggagat actgaacaat
      661 gacagaccag gataaacaca ttgagaaact gaaaaagttg ctggcgctgg ccgcatccgg
      721 caaccctcac gaggccgctc tggcactgcg ccgcgcccgt aaactgatgg atgttcacgg
      781 catcacacat tccgacatcg ccatgagtga tattgatgaa accatcagtc actactggcc
      841 gacaggcagc ctccgtccgc cgcgctacat gctgggcctg atgaacatca tccgcgaggc
      901 atttggtgtt aactccatca ttcaccccgg cacatatccg ggtgtggggt tctacggcaa
      961 ccgggaacgg gccgcactgg ccgc
//
LOCUS       DAPOZO010000259          980 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.267, whole genome shotgun sequence.
ACCESSION   DAPOZO010000259 DAPOZO010000000
VERSION     DAPOZO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.267"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>980
                     /locus_tag="ROI21_004884"
     CDS             <1..>980
                     /locus_tag="ROI21_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDX5113676.1"
                     /translation="EGVVRITDSATLTIGNGVDTTLADLTAASRGSVWLNSNNSCAGT
                     SNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTTSELSGSGNFYLHTNVAGSRG
                     DQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGGDASFTLGNTGGFVDLGTYEY
                     VLKSDGNSNWNLTNDVKPNPDPNPNPKPDPKPDPKPDPKPDPTPAPTPTPVPEKRITP
                     STAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNNVWGTTYNTRNNVTTDAGAGF
                     EQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFDRGGHGSVASYSLG"
BASE COUNT          259 a          260 c          253 g          208 t
ORIGIN      
        1 gaaggggtgg tccggattac cgatagcgcg acattaacta tcggaaatgg cgtcgatacg
       61 acgcttgccg acctgacggc tgccagtcgg ggcagtgtct ggcttaacag caataattcc
      121 tgtgcaggca ccagcaactg cgaatataga gtaaacagtt tactcctcaa cgacggtgat
      181 gtttatttat cagcacaaac agcagcgcct gccacaacta acggtatcta caatacgctg
      241 accaccagtg aactttccgg cagcggtaat ttctacctgc ataccaacgt tgcaggctcc
      301 cggggcgatc aactggtcgt caacaacaac gccactggca attttaaaat ctttgttcag
      361 gataccggcg tcagccctca gtctgacgac gcgatgacgc tggtgaaaac agggggaggg
      421 gatgcttcgt ttacgctggg taataccggc ggtttcgttg atcttgggac ctatgagtat
      481 gtcctgaaaa gcgacggcaa cagcaactgg aacctgacca atgatgtcaa acccaacccg
      541 gaccccaacc caaatccgaa accggatcca aaaccagacc caaaaccgga tccgaaacca
      601 gacccgactc ccgctccaac gccgacaccc gtcccggaga aacgcatcac gccttctaca
      661 gcagccgtac tcaatatggc agcaacatta ccgttggtct ttgatgctga gctaaacagt
      721 attcgcgagc ggttgaacat aatgaaagcg agtccacaca acaataatgt ctgggggacg
      781 acgtataaca cccgtaataa tgtcaccacc gatgcgggtg ccgggtttga gcagacgctg
      841 accggaatga cggtggggat cgatagccgt aatgatattc ctgaggggat tgcgacgctg
      901 ggcgctttta tgggttattc ccattcacat attggttttg atcgtggagg acatggcagt
      961 gtggccagtt attctctggg
//
LOCUS       DAPOZO010000260          950 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.268, whole genome shotgun sequence.
ACCESSION   DAPOZO010000260 DAPOZO010000000
VERSION     DAPOZO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.268"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..942
                     /gene="ldtE"
                     /locus_tag="ROI21_004885"
     CDS             <1..942
                     /gene="ldtE"
                     /locus_tag="ROI21_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDX5113677.1"
                     /translation="WAVDYPLPPTGSRLVGQNQTYTVQEGDKNLQAIARRFDTAAMLI
                     LEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNLAELRLYYYPPGENIVQVYPIG
                     IGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLERGIKLPPVVPAGPNNPLGRYAL
                     RLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIKALFSSVRTGTPVKVINEPVKY
                     SVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQFKDNKGVDQKLVDKALYRRAG
                     YPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ"
BASE COUNT          246 a          237 c          266 g          201 t
ORIGIN      
        1 tgggcggttg attatccgct accaccaacc ggaagccgac tggttgggca aaatcaaacg
       61 tatacggtgc aggaagggga taaaaacctt caggccatcg cccgacgttt tgatactgcg
      121 gcaatgttga tccttgaagc caataacact atcgccccgg tgccaaaacc cggtacgacg
      181 ataactattc cttcgcaact gttattacct gatgcgccgc gtcaggggat tatcgttaac
      241 ctggcagagc tgcgccttta ttattatccg ccgggagaaa atatcgtgca ggtctatccg
      301 ataggtattg gtttgcaggg gctggaaacg ccagtgatgg aaacgcgtgt ggggcagaaa
      361 atccctaacc caacctggac gcctacggcg ggcattcgtc agcgttcgct ggagcgtggc
      421 attaaattac cgccagtcgt tcctgccggg ccaaataacc cgctaggacg ttacgcactg
      481 cgcctcgcgc atggtaatgg cgaatacctc attcatggca ccagtgcgcc ggacagcgtc
      541 ggtttgcgcg tcagttcggg ctgtattcgc atgaatgcgc cggatattaa agccttattc
      601 tccagcgtgc ggacgggaac gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg
      661 gaaccgaacg ggatgcgtta tgttgaagta catcgaccac tatcagcaga agaacagcag
      721 aacgttcaga caatgccata cacactgcca gcaggcttta cgcaatttaa agacaataag
      781 ggtgtagatc agaaattagt cgataaagcg ttgtatcgtc gggcagggta tccggttgcg
      841 gtgagcagtg gagcaactcc cgcagccagc aatgcgcctt cagtagagtc agcgcagaat
      901 ggtgaaccag agcaagggaa tatgttacgc gcgacgcagt agcggtaaat
//
LOCUS       DAPOZO010000261          943 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.269, whole genome shotgun sequence.
ACCESSION   DAPOZO010000261 DAPOZO010000000
VERSION     DAPOZO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.269"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..850
                     /gene="yeeO"
                     /locus_tag="ROI21_004886"
     CDS             <1..850
                     /gene="yeeO"
                     /locus_tag="ROI21_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDX5113678.1"
                     /translation="GLFSWPGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISL
                     KSYFKPLNFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIA
                     ALINLPGSALGSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGL
                     MASFYTQDPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWG
                     CRVVVGYVLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEK
                     KPVVSE"
BASE COUNT          188 a          190 c          274 g          291 t
ORIGIN      
        1 cggccttttc tcctggccgg gactgggatt tgtcggggca gggctgggtt taaccatttc
       61 tcgttatatt ggcgcagttg caattttgtg ggtgctggcg attggtttta atcctgcgct
      121 aaggatttcg ttaaagagct attttaaacc gctgaatttt agcattatct gggaagtcat
      181 ggggattggt attcccgcga gtgtcgaatc agtgttattt accagtggtc ggttattaac
      241 ccaaatgttc gttgccggga tggggaccag tgttattgcc ggaaatttta tcgcgttttc
      301 aattgcggct cttatcaact tacccggaag tgcgctcggc tctgcttcta cgatcattac
      361 aggccgaagg ttgggggtag ggcagatagc gcaagcagag attcagttgc ggcatgtgtt
      421 ctggctgtcc actcttggat taacggccat cgcctggcta acggctccct ttgccgggct
      481 tatggcatcg ttttacaccc aggatccaca ggttaaacat gtcgttgtga ttctgatttg
      541 gctaaatgct ttatttatgc ctatttggtc cgcctcatgg gtgctacccg ctggatttaa
      601 aggtgctcgt gatgcccgtt acgccatgtg ggtttcgatg ttgagcatgt ggggttgtcg
      661 ggttgtagtc ggttatgtgc tgggaatcat gcttggctgg ggtgtggttg gtgtctggat
      721 gggaatgttt gccgactggg ctgtgcgggc cgtgctgttt tactggcgaa tggttactgg
      781 acgttggcta tggaaatacc ctcgacccga gccgcaaaag tgtgaaaaaa agccagttgt
      841 gtcggaataa acgacaaaat gcagattatt tcagcaaacg atttcaaatt taaaaaacag
      901 gctttgacat tgtgggtggg catcgctaat attcgcctcg ttc
//
LOCUS       DAPOZO010000262          938 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPOZO010000262 DAPOZO010000000
VERSION     DAPOZO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.270"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>938
                     /locus_tag="ROI21_004887"
     CDS             <1..>938
                     /locus_tag="ROI21_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HDX5113679.1"
                     /translation="TNSPQGPWWVLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRT
                     QTFHREAGGEITGVTDGAGRHFRLVLTTQAQRAEEARQQASSGGTEPSAFPDTLPGYT
                     EYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYD
                     DKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSMDRRE
                     VLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITR
                     ITTPDGRASAFYYNHHNQLTSATGPDGLEIRREYDES"
BASE COUNT          200 a          273 c          320 g          145 t
ORIGIN      
        1 acgaacagtc cgcaggggcc gtggtgggta ctcggctggt gtgagcgggt gccggaggcg
       61 gatgaggtgc tgcctgcgcc gctgccgccg taccgggtac tgaccgggct ggtggaccgc
      121 ttcgggcgca cacagacgtt ccaccgtgag gctggcggcg aaatcaccgg cgtgacggat
      181 ggtgccgggc gtcacttccg gctggtactg accacgcagg cgcagcgggc agaagaagcc
      241 cggcagcagg cctcttccgg cgggacggaa ccgtccgctt ttcctgatac cctgccgggt
      301 tacaccgaat atggccggga caacggcatc cgtctgtctg ccgtgtggct gacgcacgac
      361 ccggaatacc cggagaattt acctgccgcg ccgctggtgc gctatggctg gacgccacgc
      421 ggcgaactgg cggcggtgta tgaccgcagc aatacacagg tgcgcagctt tacttacgat
      481 gataaatacc ggggccggat ggtggcgcac cgtcacacgg gccggccgga aatccgttac
      541 cgttacgaca gcgacgggcg ggtgacagaa cagctaaacc cggcaggctt aagctacacg
      601 tatcagtatg agaaagaccg catcaccatc accgacagta tggaccgccg tgaagtgctg
      661 cacacgcagg gcgaaggcgg gctgaagcgg gtggtgaaaa aggaacacgc ggacggcagc
      721 gtcacgcaga gtcagtttga tgcggtgggc aggcttaagg cacagacgga tgccgcaggc
      781 cggacgactg agtacagccc ggatgtggtg acgggactca tcacgcgcat caccacaccg
      841 gatggcaggg catcggcatt ttactataac caccacaacc agttaacgtc agccaccggg
      901 cctgacgggc tggaaatacg ccgggaatat gatgaatc
//
LOCUS       DAPOZO010000263          932 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.271, whole genome shotgun sequence.
ACCESSION   DAPOZO010000263 DAPOZO010000000
VERSION     DAPOZO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.271"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            53..259
                     /locus_tag="ROI21_004888"
     CDS             53..259
                     /locus_tag="ROI21_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113680.1"
                     /translation="MTTETIVCFLFWYMYAGSCSARLHRSLGYGRHYDTAHYILYMTA
                     VMLFWPVTLPAATDIVADRLKKRR"
     gene            261..710
                     /locus_tag="ROI21_004889"
     CDS             261..710
                     /locus_tag="ROI21_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HDX5113681.1"
                     /translation="MQKKRLIQLIHIARNELGMDEDTYRQMLQGLTGKASTKGMDTTQ
                     LNCVLESMKKKGFRVKPARKASSGLPLDNHPQSRKIRALWLEMAAAGIVRDRSENALA
                     RWIKRETGISALRWLSTEQASSVIEKLKKWQRRAAGVKHERPESVSK"
     gene            682..>932
                     /locus_tag="ROI21_004890"
     CDS             682..>932
                     /locus_tag="ROI21_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDX5113682.1"
                     /translation="MSDLNQFRSKGPELLVELAQHTSETVREIIDIEPEVADQIGQAV
                     ANRMMQVWGGQNVYFPMGMVWKVSQRDREIFREFNGRNH"
BASE COUNT          254 a          226 c          251 g          201 t
ORIGIN      
        1 gaccctgtca ttgctgcccg taatattcat atcgaatggg gaggtattca gaatgactac
       61 tgaaaccatt gtctgttttc ttttctggta tatgtacgca ggatcatgca gcgcaagact
      121 tcatcgttcc ctgggctatg gcaggcacta cgacaccgcg cattacatcc tgtacatgac
      181 cgccgtcatg ttgttctggc cagtcaccct gccagctgca accgacattg ttgccgacag
      241 actgaaaaaa aggagatgat atgcagaaaa aacgcctgat acagcttatc catattgccc
      301 gtaatgaact gggtatggat gaagacacct accgccagat gttacagggg ctgaccggta
      361 aagcctcaac caaaggaatg gataccacac aactaaactg cgtgctggaa tccatgaaaa
      421 agaaaggctt tcgcgttaaa ccagccagaa aagccagctc cggtttaccg cttgataacc
      481 atccgcagtc caggaaaatt cgtgcgctat ggcttgaaat ggctgctgcc ggcattgttc
      541 gtgaccgttc agaaaatgca ttagcgaggt ggatcaagcg ggaaacgggc atcagcgccc
      601 tgcgctggct cagtactgaa caggcaagca gtgttattga gaaactgaag aagtggcagc
      661 gcagagctgc gggagtcaaa catgagcgac ctgaatcagt ttcgaagtaa ggggccggaa
      721 ctcctggtgg aactggcaca gcatacctct gagaccgttc gcgagattat tgatattgag
      781 cccgaagttg ccgaccagat tggtcaggcc gtcgcgaacc gaatgatgca ggtctggggc
      841 gggcaaaacg tttatttccc gatgggcatg gtatggaagg tcagtcagcg cgaccgggaa
      901 atcttcaggg agtttaacgg acgcaaccac ca
//
LOCUS       DAPOZO010000264          932 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPOZO010000264 DAPOZO010000000
VERSION     DAPOZO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.272"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..251)
                     /locus_tag="ROI21_004891"
     CDS             complement(<1..251)
                     /locus_tag="ROI21_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDX5113683.1"
                     /translation="MSDLNQFRSKGPELLVELAQHTSETVREIIDIEPAIADQIGQAV
                     ANRMMQVWGGQNVYFPMGMVWKVSQRDREIFREFNGRNH"
     gene            complement(223..672)
                     /locus_tag="ROI21_004892"
     CDS             complement(223..672)
                     /locus_tag="ROI21_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HDX5113684.1"
                     /translation="MTKQRLIQLIHIARNELGMDEDSYRQMLQGLTGKASTKGMDTTQ
                     LNCVLESMKRKGFRVKPARKASSGLPLDNHPQSRKIRALWLEMAAAGIVRDRSENALA
                     RWIKRETGISALRWLNTEQASSVIEKLKKWQRRAAGVKHERPESVSK"
     gene            complement(665..880)
                     /locus_tag="ROI21_004893"
     CDS             complement(665..880)
                     /locus_tag="ROI21_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113685.1"
                     /translation="MTAESVVCALFWYCFVGWCTAELHRRSGFYSRYSGAGYWISWSV
                     MFLCWPVALPLYVDYIGDAGRRSNDDD"
BASE COUNT          222 a          266 c          212 g          232 t
ORIGIN      
        1 tggtggttac gtccgttaaa ctccctgaag atttcccggt cgcgctgact gaccttccat
       61 accatgccca tcgggaaata aacgttttgc ccgccccaga cctgcatcat gcggttcgcg
      121 acggcctgac caatctggtc ggcaattgcg ggctcaatat caataatctc gcgaacggtc
      181 tcagaggtat gctgtgccag ttccaccagg agttccggcc ctttacttcg aaactgattc
      241 aggtcgctca tgttttactc ccgcagctct gcgctgccac ttcttcagtt tctcaataac
      301 actgcttgcc tgttcagtat tgagccagcg cagggcgctg atgcccgttt cccgcttgat
      361 ccaccgcgct aatgcatttt ctgaacggtc acgaacaatg ccggcagcag ccatttcaag
      421 ccatagcgca cggattttcc tggactgcgg atggttatcc agcggtaaac cggagctggc
      481 ttttctggca ggcttaacgc gaaagccttt ccttttcatg gattccagca cgcagtttag
      541 ttgtgtggta tccattcctt tggttgaggc tttaccggtc agcccctgta acatctggcg
      601 gtagctgtct tcatccatac ccagttcatt acgggcaata tgaatgagct ggataagacg
      661 ttgtttagtc atcatcgttg ctccttctgc ctgcatcacc gatataatcg acatataaag
      721 gaagtgccac aggccagcac aggaacatca ccgaccagct aatccagtaa ccggcaccac
      781 tgtaacgtga ataaaaccct gaacggcggt gcagttcagc agtacaccaa ccgacaaaac
      841 aataccagaa cagggcacat acaacagatt cagccgtcat tctgaatatc cccccaactg
      901 atatgaatat tacgggcagc aatgacaggg tc
//
LOCUS       DAPOZO010000265          922 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPOZO010000265 DAPOZO010000000
VERSION     DAPOZO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.273"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            68..>922
                     /locus_tag="ROI21_004894"
     CDS             68..>922
                     /locus_tag="ROI21_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HDX5113686.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSG"
BASE COUNT          157 a          297 c          294 g          174 t
ORIGIN      
        1 attgaatata accgacgtga ctgttacatt taggtggcta aacccgtcaa gccctcagga
       61 gtgaatcatg accgtagtca cgaccgccga tacctcccaa ctgtacgcac ttgcagcccg
      121 acatgggctc aagctccatg gcccgctgac tgtcaatgag cttgggctcg actataggat
      181 cgtgatcgcc accgtcgacg atggacgtcg gtgggtgctg cgcatcccgc gccgagccga
      241 ggtaagcgcg aaggtcgaac cagaggcgcg ggtgctggca atgctcaaga atcgcctgcc
      301 gttcgcggtg ccggactggc gcgtggccaa cgccgagctc gttgcctatc ccatgctcga
      361 agactcgact gcgatggtca tccagcctgg ttcgtccacg cccgactggg tcgtgccgca
      421 ggactcggag gtcttcgcgg agagcttcgc gaccgcgctc gccgccctgc atgccgtccc
      481 catttccgcc gccgtggatg cggggatgct catccgtaca ccgacgcagg cccgtcagaa
      541 ggtggccgac gacgttgacc gcgtccgacg cgagttcgtg gtgaacgaca agcgcctcca
      601 ccggtggcag cgctggctcg acgacgattc gtcgtggcca gatttctccg tggtggtgca
      661 tggcgatctc tacgtgggcc atgtgctcat cgacaacacg gagcgcgtca gcgggatgat
      721 cgactggagc gaggcccgcg ttgatgaccc tgccatcgac atggccgcgc accttatggt
      781 ctttggtgaa gaggggctcg cgaagctcct cctcacgtat gaagcggccg gtggccgggt
      841 gtggccgcgg ctcgcccacc acatcgcgga gcgccttgcg ttcggggcgg tcacctacgc
      901 actcttcgcc ctcgactcgg gt
//
LOCUS       DAPOZO010000266          908 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPOZO010000266 DAPOZO010000000
VERSION     DAPOZO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.274"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>908
                     /gene="lrhA"
                     /locus_tag="ROI21_004895"
     CDS             <1..>908
                     /gene="lrhA"
                     /locus_tag="ROI21_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDX5113687.1"
                     /translation="SANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQQM
                     QRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLTIG
                     ASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSTFK
                     ALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYVAS
                     TLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNELAQ
                     VIYQAMESYHNPWQYSPMSAPEGDDS"
BASE COUNT          215 a          225 c          231 g          237 t
ORIGIN      
        1 aagtgcaaat cgtccgataa ttaacctcga cctcgatctg ctgagaacat ttgttgctgt
       61 tgccgatctg aacacttttg ctgccgcagc tgccgctgtg tgtcgtactc agtccgccgt
      121 aagtcagcaa atgcagcgtc tggaacaact tgttgggaaa gaactgttcg ctcgtcacgg
      181 tcgcaacaaa ctgttaactg aacatggcat tcaacttctt ggttacgcca gaaaaattct
      241 gcgttttaat gatgaggcct gctcatcatt aatgttcagt aatcttcagg gcgtgttaac
      301 tatcggtgct tcagatgaat ctgccgatac gatcttacct ttcctgttaa atcgcgtgag
      361 ttcggtttat ccgaaactag cgctggatgt ccgcgttaaa cgtaatgcct atatggcaga
      421 aatgctggaa tcccaggaag tggatttgat ggtaaccacc catcgtcctt caacgtttaa
      481 ggcattgaac ctgcgtacct cgccaacaca ctggtactgc gcagcggaat acgtattgca
      541 aaaaggcgag ccgatccctc ttgtactgct ggatgatcct agcccgtttc gcgatatggt
      601 gctggcgacg cttaataaag cagatattcc atggcgactg gcttatgtcg cctcgacgct
      661 tccggcagtt cgtgcggcag taaaagccgg tcttggcgtg acggcaaggc cggtcgagat
      721 gatgagtcca gacctgcgtg tgctgagtgg agtagatggc ttaccgccgc tacccgatac
      781 cgaatatttg ctgtgctatg acccgtccag taataatgag ctggctcagg tgatctatca
      841 ggcgatggag agctaccata atccgtggca atacagccca atgtctgctc cggaaggcga
      901 tgactcat
//
LOCUS       DAPOZO010000267          890 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.275, whole genome shotgun sequence.
ACCESSION   DAPOZO010000267 DAPOZO010000000
VERSION     DAPOZO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.275"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            13..>890
                     /locus_tag="ROI21_004896"
     CDS             13..>890
                     /locus_tag="ROI21_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDX5113688.1"
                     /translation="MTLAESGERKTAVDKLLMEPLYQQEMLLYSRHKNELTTWKNKEE
                     LLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFIFNDATTAAIKD
                     QLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNEPEQMIENARMT
                     LSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGAVTPGGSLTAFH
                     ERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSGPLSQFRGHVSK
                     KTENIARIAALLQYFS"
BASE COUNT          279 a          171 c          211 g          229 t
ORIGIN      
        1 tctcttttct tactgacgct tgctgagtcc ggagaacgaa aaaccgcagt ggacaaatta
       61 ttaatggagc ccctgtacca gcaggagatg ctgttatatt ccagacacaa aaatgaactg
      121 acgacatgga aaaataagga agagctttta aaggcgcaga aaaaagcatt gttgtcaaaa
      181 ctgaataaag aactccgcaa aggcgcggat gagtcagaaa cgctcagaca actggaagcg
      241 cttcagaaaa acagagggga aaaacctgta agatataaat tcatttttaa tgatgccacg
      301 acagcagcaa ttaaagatca gctttgtggt cagtggcgtt cagtgggtat catgtctgat
      361 gaagccggta taatatttga tgggtatacc ctgtctgagc tgccttttat taacaaaatg
      421 tgggatggct ccgtattgtc ggtggacaga aagaatgaac ccgaacagat gattgaaaat
      481 gccagaatga cattatccct catggtacaa cccggtcttt ttgaccgata tatggagcga
      541 aaaggttctg ttgccaggga cagtggtttt cttgcccgtt gtctaatcag taaacctgcc
      601 accacgcagg ggaaacgctt tattaacgga gctgtgaccc cgggaggatc attaacggca
      661 tttcatgaac gattaatgga actggccaga ggcagtattg aaaaatccag taaggatgaa
      721 cgttattgtc tgcatttttc tcctgaagcg caaaaaatat ttattgatca ctataatgtt
      781 ctggagcagg atttgtctcc ttccggaccc ttaagccagt ttcgggggca tgtttcaaaa
      841 aaaacagaaa acattgcgag aattgctgcg ttattgcagt atttcagtca
//
LOCUS       DAPOZO010000268          866 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.276, whole genome shotgun sequence.
ACCESSION   DAPOZO010000268 DAPOZO010000000
VERSION     DAPOZO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.276"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>866
                     /locus_tag="ROI21_004897"
     rRNA            <1..>866
                     /locus_tag="ROI21_004897"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          220 a          195 c          285 g          166 t
ORIGIN      
        1 gagtaatgtc tgggaaactg cctgatggag ggggataact actggaaacg gtagctaata
       61 ccgcataacg tcgcaagacc aaagaggggg accttcgggc ctcttgccat cggatgtgcc
      121 cagatgggat tagctagtag gtggggtaac ggctcaccta ggcgacgatc cctagctggt
      181 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
      241 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      301 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      361 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      421 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      481 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      541 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      601 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      661 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      721 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      781 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      841 tttaattcga tgcaacgcga agaacc
//
LOCUS       DAPOZO010000269          851 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPOZO010000269 DAPOZO010000000
VERSION     DAPOZO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.277"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..333
                     /locus_tag="ROI21_004898"
     CDS             <1..333
                     /locus_tag="ROI21_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HDX5113689.1"
                     /translation="EEKHDAMQSLVLSPPARQALAQAALTYRYGDEHQPVTTADILTP
                     RRREDYGQDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMA
                     ETLLESLR"
     gene            675..>851
                     /locus_tag="ROI21_004899"
     CDS             675..>851
                     /locus_tag="ROI21_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDX5113690.1"
                     /translation="MKTLSQNTTSSACAPETDLQKLVATPVPDERRISFWPQHFGLIP
                     QWVTLEPRIFGWMDR"
BASE COUNT          196 a          251 c          222 g          182 t
ORIGIN      
        1 gaggagaagc atgatgccat gcagtcgctg gtcctgtcgc caccggcacg ccaggcgctg
       61 gcacaggcgg cactgactta ccgttatggt gacgaacatc agccagtcac caccgctgac
      121 attctgacac cacgacgcag ggaggattac ggtcaggacc tgtggagcgc atatcagacc
      181 atccaggaga atatgctgaa aggcggaatt tccggccgca gtgcaaaagg aaaacgtatc
      241 cacacccgtg ccattcacaa catcgacacc gatattaagc tcaaccgcgc attgtgggta
      301 atggcagaaa cgctgctgga gagcctgcgc tgatgcagca tgtccctgaa agcgtgattc
      361 aggcaccaga atcacccgac gccccggagg cgctgccggg gtgtcgttcc atttctgatg
      421 atgacctgtg cgcctggtgt acccgtctgc tttaccgtcc cggagaaatc agtctgtgca
      481 gactgagtat acaggcaatg gcatctggcc atccgtctgt gaccggaacg gttacgcgta
      541 cggctgtccg gaatttcagc cgaatatcat tcatccctga tattaccggg tcatcacggt
      601 cagtgatgaa cgtgatgttg ccagctcccg acctttactc cctctcattt attcacatta
      661 caaggatttc atacatgaaa acattgtctc agaacaccac ctcttcggcc tgtgcaccgg
      721 agactgacct gcaaaaactg gtagccactc ccgtccctga tgaacggcgc atcagcttct
      781 ggccgcagca ctttggcctc attccacagt gggtgaccct ggagccccgt atcttcggct
      841 ggatggaccg t
//
LOCUS       DAPOZO010000270          821 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPOZO010000270 DAPOZO010000000
VERSION     DAPOZO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.278"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..800)
                     /locus_tag="ROI21_004900"
     CDS             complement(<1..800)
                     /locus_tag="ROI21_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN/FAD transporter"
                     /protein_id="HDX5113691.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAVAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGL"
BASE COUNT          259 a          209 c          173 g          180 t
ORIGIN      
        1 aggccgtaaa tcaatatgcc gctaataata atattaagaa tattcaggct accgttaatc
       61 agtagcggta ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat caaagtgatg
      121 gcaactgctg gataactgag taccgtcagc tccagataag tcaacgccag tgctttaact
      181 tctgtcgtgg catcacccgc gacgaaatca ataatttgtt cgccaaaatg atgaataagc
      241 gttgccaaca gtacggcaaa caacgtcatg atcaccaatg actgccgcgt cgccaccctc
      301 gctcgtcgtc gatcccgctt accgagacta aatgccacaa cgacagtagt accaagatcg
      361 atagcagcaa aaaaagccat aatgaccata ttgaagctgt ccgccaatcc cacgccggcc
      421 atcgcatctt ttcccagcca gctgaccaga aaagtgctca ggacacccat caacaggaca
      481 caggcattct ccatgaagat aggaacagca agcggggtta tctcgcgcca gaacaacact
      541 ttgtagctct tgcgtttagc gtgccagcga gtgccgtgaa caacctggcg taaagcagag
      601 gagatattca aagccgacct taattgcaga aagtgaaacc acatttcaaa taatgaggga
      661 gaatcagcaa agctgcaaag attttcgcca acaaattgtc tgcaaatgca acaaactgtt
      721 gatagaaacg gcaaacagtt gggaaattta aaaatcgggt ttgacaaaag atttttcgcc
      781 gttaagatgt gcctcaacag cgattcctct gtagttcagt c
//
LOCUS       DAPOZO010000271          812 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPOZO010000271 DAPOZO010000000
VERSION     DAPOZO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.279"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>812
                     /gene="ag43"
                     /locus_tag="ROI21_004901"
     CDS             <1..>812
                     /gene="ag43"
                     /locus_tag="ROI21_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDX5113692.1"
                     /translation="ARGGSLAGTTTLNNGATLTLSGKTVNNDTLTIREGDALLQGGAL
                     TGNGRVEKSGSGTLTVSNTTLTQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGST
                     VLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLQVK
                     NLNGQSGIISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQ
                     VVEAINGATTEEGAFVQGNMLQAGAFNYTLNRDSDESWYLRSEERYRAEVPL"
BASE COUNT          199 a          236 c          237 g          140 t
ORIGIN      
        1 gccagagggg gctcgctggc gggcaccacc acgctgaata acggtgccac gcttaccctt
       61 tccgggaaaa cggtgaataa cgataccctg accatccgtg aaggtgatgc actcctgcag
      121 ggaggcgctc ttaccggtaa cggcagggtg gaaaaatcag gaagtggcac actcactgtc
      181 agcaacacca cactcaccca gaaaaccgtc aacctgaatg aaggcacgct gacgctgaac
      241 gacagtaccg tcaccacgga tgtcatcgct cagcgcggca cggccctgaa gctgaccggc
      301 agcacagtgc tgaacggtgc cattgacccc acgaatgtca ctctcgcctc cggtgccacc
      361 tggaatatcc ctgataacgc cacggtgcag tcggtggtgg atgacctcag ccatgccgga
      421 cagattcatt tcacctccac ccgcacaggg aagttcgtac cggcaaccct gcaggtgaaa
      481 aacctgaacg ggcagagtgg catcatcagc ctccgtgtac gcccggatat ggcgcagaac
      541 aatgctgaca gactggtcat tgacggtggc agggcaaccg gaaaaaccat cctgaatctg
      601 gtgaacgccg gcaacagtgg tacggggctg gcgaccaccg gtaagggtat tcaggtggtt
      661 gaggccatta acggtgccac cacggaggaa ggggcttttg tccaggggaa tatgctgcag
      721 gccggggcct ttaactacac cctcaaccgg gacagtgatg agagctggta tctgcgcagt
      781 gaagaacgtt atcgtgctga agtccccctg ta
//
LOCUS       DAPOZO010000272          722 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.280, whole genome shotgun sequence.
ACCESSION   DAPOZO010000272 DAPOZO010000000
VERSION     DAPOZO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.280"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT          268 a          169 c          100 g          185 t
ORIGIN      
        1 aatacttctt ccctgctgac acagataaaa tgcaccattt tttcttcaaa tacttctgta
       61 taatcgccag ccaatgatga gaatggatta aaataacact tcttcgacaa acacaaaaac
      121 agccttcatt ttacatcatc atccagtttc agttaacccc ggacgagcga cagtgtttca
      181 gtacaggaat taaaagccag cttttgctca gtaaaacaca ctcaactaat aatatctcag
      241 cagatattat cctgaccagg acacctcaca ttactgaatt ctcttccgac agtatcactg
      301 cacaaataaa aagaacggga taaatattca gaccggaaga atggtgtcac aaaagcattt
      361 aatcattacg catgaccagg aagggaaaca cttaaccaca tggtatcaac agatatcatt
      421 acgtgtgcac tcttataaaa acacacagaa gaagtaagat aattataaac gtaaatttaa
      481 tatctcccac tgacatctcc cccacaatac actaaaaaat actcacagca gtaaacacag
      541 atacacctga taatcataca gccgataaat attttcccct ttacggagaa atcagcctgc
      601 tgtttaacaa taacggcgta ttttcatgga aggagatcac ccagtaacgg ataagtcctg
      661 atctccttca tgttaatacg caacaatccc atcgattcaa aacaatttca gacagatatt
      721 at
//
LOCUS       DAPOZO010000273          719 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPOZO010000273 DAPOZO010000000
VERSION     DAPOZO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.281"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>719
                     /locus_tag="ROI21_004902"
     CDS             <1..>719
                     /locus_tag="ROI21_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDX5113693.1"
                     /translation="QTRSYSYSDSGRLTGVHTTTSNLDIRIPYATDPAGNRLPDPELH
                     PDITLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQHR
                     LVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGD
                     RLTTIQNDRTRIQTVYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVV
                     FPPVLVQMLDRLESETLADRV"
BASE COUNT          157 a          228 c          226 g          108 t
ORIGIN      
        1 cagacccgga gttacagcta cagcgactcc ggcaggctga ccggcgtcca caccaccacc
       61 tcaaatctgg atatccgcat cccgtatgcc acggacccgg caggcaaccg cctgcccgac
      121 ccggagctgc acccggacat caccctcagc atgtggccgg ataaccgtat cgcccgtgac
      181 gcgcactatc tttaccggta tgaccgtcac ggcagactga cggagaaaac cgacctcatc
      241 ccggaagggg ttatccgcac ggatgatgag cggactcacc ggtaccatta cgacagtcag
      301 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
      361 ctttacgacc cgctgggccg cagggtggca aaacgggtgt ggcggcgtga acgggacctg
      421 acgggctgga tgtcgctgtc acggaaaccg gaagtgacct ggtacggctg ggacggcgac
      481 cggctgacca cgatacagaa cgacagaacc cgcatccaga cggtttatca gccggggagc
      541 ttcacgccac tcatcagggt cgaaaccgcc accggtgagc tggcgaaaac gcagcgccgc
      601 agcctggcgg atgcgcttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      661 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa ccctggctga ccgggtgag
//
LOCUS       DAPOZO010000274          706 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPOZO010000274 DAPOZO010000000
VERSION     DAPOZO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.282"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            142..561
                     /locus_tag="ROI21_004903"
     CDS             142..561
                     /locus_tag="ROI21_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113694.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYAHYTCHLCGSALRYHPQH
                     DTERPWFEHTDDGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGEQYCTHCQTGRFSEDGEQNERYKNGAGDHAG"
     gene            521..>706
                     /locus_tag="ROI21_004904"
     CDS             521..>706
                     /locus_tag="ROI21_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113695.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQK"
BASE COUNT          165 a          199 c          192 g          150 t
ORIGIN      
        1 tcagtgtgat gacgtgttca cagcgaccac actgattttc ccttctgaga ctgaactgaa
       61 aaccgccatt cacacctgac cacgctgccc gtaccgggac ggctgttgcc tgcgtgtcgt
      121 ctggcggtaa ggaaccacac tatgtacgca aaatcttttc tcgcacttga tggcaacgga
      181 cgtctgaccg gtgcccgtac cgcacagact gctccttatg cccactacac ctgccacctt
      241 tgcggcagtg cactcagata ccacccgcag cacgacacgg aacgtccctg gtttgaacac
      301 actgacgacg ggctgacgga gcacgcgcag caatgtcctt atgtcaggcc ggaacgcaga
      361 gaaatacggt tgattaaacg tctgcagcaa ttcgtcccgg atgccttacc cgtggtacgt
      421 aaagccagct ggtactgcag gcaatgtcac cacgattatt atggggagca gtactgcaca
      481 cactgccaga ccgggcgctt cagtgaggac ggggagcaga atgaacggta taaaaacggg
      541 gctggggatc acgccgggtg agcacattat cagtgccgac tctgcgctca gcagaaatat
      601 ccggcattgt ttctgccttt cctgtcatgg ccggttgatt ctgcagacag atgctcaggg
      661 ggcgtggttt gagcatgatt tacatgccct gagtgagcaa cagaag
//
LOCUS       DAPOZO010000275          698 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPOZO010000275 DAPOZO010000000
VERSION     DAPOZO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.283"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..429
                     /locus_tag="ROI21_004905"
     CDS             <1..429
                     /locus_tag="ROI21_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HDX5113696.1"
                     /translation="QSLGEVRVPHRGNVVEKVIEGAYEVVGVFDRIEEKRDAMQSLVL
                     PPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGI
                     SGRSAKGKRIHIRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            511..>698
                     /locus_tag="ROI21_004906"
     CDS             511..>698
                     /locus_tag="ROI21_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDX5113697.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRL"
BASE COUNT          164 a          181 c          197 g          156 t
ORIGIN      
        1 cagtctctgg gggaagtgcg tgttccacac cggggaaatg tagtggagaa agttatcgaa
       61 ggggcttacg aggtggtggg cgtgtttgac cggatagagg agaagcgtga tgccatgcag
      121 tcgctggtcc tgccgccacc ggcacgccag gcgctggcac aggcggcact gacttaccgt
      181 tatggtgacg aacatcagcc cgtcaccacc gccgacattc tgacgccgcg acgccgggag
      241 gattacggta aggacctgtg gagcgcatat cagaccatcc aggagaatat gctgaaaggc
      301 gggatttccg gtcgcagtgc aaaaggaaaa cgtatccata tccgggccat tcacagcatt
      361 gataccgaca ttaagctcaa tcgcgcattg tgggtgatgg cagaaacgat gctggagagc
      421 ctgcgctgat gccgtttccc tgagtgaggg aatcattcag ctttcgttcc tgaaaaacac
      481 acatttattt gttatttaag gagttatctc atgaaaaccg tttctcagaa tacccccaca
      541 atttattcag ctacaacacc agagaataat ccgcctcagt tggttgccag cctcgtccct
      601 gatgaacagc gcatcagctt ctggccgcag cattttggcc tcattccaca gtgggtgacc
      661 ctggagcccc gtgtcttcgg ctggatggac cgtctgtg
//
LOCUS       DAPOZO010000276          694 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPOZO010000276 DAPOZO010000000
VERSION     DAPOZO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.284"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>694
                     /locus_tag="ROI21_004907"
     CDS             <1..>694
                     /locus_tag="ROI21_004907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113698.1"
                     /translation="SRIKPLFLIVIAYIKRKIHSKNDHAIYFGIEDYKGISSCVYKFS
                     FCANQAIWLWNPRRTLNISEIEFKILLITLKITGVQVWTFDYNDSVKYKLNYHPQVYS
                     LEFSRQVLNKKLCEELTNPVMFDIFFVGVDKGRLELLNTFAKLLDDASLSFSISVLPD
                     KNKHYDECERLLAKNAMNYDEYLDKLFQTTAILEIVQPGQGGLTIRTLEALFYNKKLI
                     TNNESITEYDFY"
BASE COUNT          251 a           91 c          118 g          234 t
ORIGIN      
        1 aatcaaggat aaaaccatta tttttgattg tcattgcgta tataaaaagg aaaattcact
       61 caaaaaatga tcatgcaatt tattttggta tagaggatta taaaggaata tcttcttgcg
      121 tatataagtt ttcgttttgt gccaatcaag ccatttggtt atggaatcct agacgaacat
      181 tgaacatctc agaaatagag tttaagattc tattaatcac attaaaaatt actggggttc
      241 aagtttggac gtttgattat aatgactcgg ttaaatataa actaaattat catccccaag
      301 tttactcatt ggaatttagt cgtcaagtat taaataaaaa actgtgtgag gaattaacca
      361 atcctgtaat gttcgatatt ttctttgttg gggtagataa agggcggctt gaacttttaa
      421 atacctttgc aaagttatta gacgatgctt ccttgagctt ttcaattagt gttttgcctg
      481 ataaaaataa gcattatgat gaatgtgaga gactattagc aaaaaatgca atgaattatg
      541 acgagtatct tgataaatta tttcaaacaa cagctattct tgaaattgtt caaccgggac
      601 aaggaggtct gacaattaga acattggaag ccctattcta taataaaaag ttaataacaa
      661 ataacgaaag tattaccgaa tatgattttt ataa
//
LOCUS       DAPOZO010000277          694 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPOZO010000277 DAPOZO010000000
VERSION     DAPOZO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.285"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            34..492
                     /locus_tag="ROI21_004908"
     CDS             34..492
                     /locus_tag="ROI21_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDX5113699.1"
                     /translation="MLQCGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWEVDTLVSALMTALPDHAASPLMTRLQDELRTESVRA
                     QAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          142 a          192 c          189 g          171 t
ORIGIN      
        1 gccctgtctg tggatgagta tttctggcga cacatcctgc agtgcggaca tcagcaggtg
       61 ctgtttgtgg tgacacaggc cgacaaaacg gagccctgcc atgaatggga tatggccggc
      121 attcagccct ctcccgcaca ggcacagaac attcgcgaaa aaacggaggc ggtattccgt
      181 ctgttccggc ctgtacatcc ggttgtggcc gtatcggccc gcaccggctg ggaagtggat
      241 acgctggtca gtgcgctcat gacagcgctt cccgaccatg ccgccagtcc cctgatgact
      301 cgcctgcagg acgagctgcg cacggaatct gtccgcgctc aggcccgtga gcagtttacc
      361 ggtgcggtgg accggatatt tgacacagcg gagagcgtct gtgttgcctc tgttgcacgc
      421 acggttctgc gtgccgtccg tgacacagtg gtctctgttg cccgcgcggt atggaactgg
      481 atcttcttct gaacctgttg tggatgatgt cctccctgcc tctgagtctg ctcacaaaag
      541 cgctgttttc gttactgtct ctcttgtccg tgcaatagct tgataataga ataaaacgat
      601 caatacctat tttaccgatc gttaatatcg atcgttatgc taataataac tcctgttagc
      661 aacgtgcgca gatacacaca gacatgagat tcag
//
LOCUS       DAPOZO010000278          681 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPOZO010000278 DAPOZO010000000
VERSION     DAPOZO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.286"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT          183 a          156 c          186 g          156 t
ORIGIN      
        1 ttgttgcgaa aagatgcttc cgcacgcacg cgcgataaag aggggatagt tttcgtgagg
       61 acagggagag aagcaggcgt tctgataatt gcagagttgt tttcggaggt ggttacagtg
      121 agtcagcccg ttcctggcga gcaggagact gatatcaccg atgggataag gttgtctgat
      181 tggtattata aatataagaa gtcatcagag agtaacgtaa aggatttgcc agaataacct
      241 aagagagtaa atctctccgg taacagagcg ctgtgaagca ttctgtatca gagtgcagat
      301 acatgaacgc tgatgcgtaa aatcagcagt gacacgccag ctccccccat gcccattcct
      361 gtataagaca tgaactgagc gtattaatgc ttctgccctg agactgagcc ggtgagtgag
      421 gggctgagaa atccctcttg acacagctct aaccagaaat acttccccgg ttgttgcggt
      481 gcgtccggat ccccgataac ggaacactaa ggctgtattg cgggacaccg ggtaagtggc
      541 agcccgtaag gcgttctgtc aacgccagaa aaaatgattt cccacacgcg ttatagactc
      601 actcctgtgg acctggaagc ccgtaactct gcggttgccc actgactgac ggaactgacc
      661 gcaaatttta cgagtgcgtg c
//
LOCUS       DAPOZO010000279          680 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPOZO010000279 DAPOZO010000000
VERSION     DAPOZO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.287"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            548..>680
                     /locus_tag="ROI21_004909"
     CDS             548..>680
                     /locus_tag="ROI21_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDX5113700.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     "
BASE COUNT          219 a          120 c          120 g          221 t
ORIGIN      
        1 gttatctgtt tgttaagtca agcaatctat ttgcaacccc accataacga tcatcctgtt
       61 acggaatatt acactgcaac atttgcgcac aaaaaataat ccgcattctt attgcggatt
      121 agttttttct tagctgatag cacaattttc atactatttt ttggcgttct ggatgtctga
      181 acgaggattt agtgccaggt cgataaagtt tccatcagaa acaaaatttc cgtttagtta
      241 atttaaatat aaggaaatca tataaataga taaatattgc tgtaaatatc atcacgcttc
      301 tatggaaata tgacggtgtt cacaaagttc cttaaatttt actttttgtt acatattttt
      361 tctttttgaa accaaatctt tatctttgta gcactttcac ggtagcgaaa cgttagtttg
      421 aatggaaaga tgcctgcaga cacataaaga caccaaactc tcatcaatag ttccgtaaat
      481 ttttattgac agaacttatt gacggcagtg gcaggtgtca taaaaaaaac catgagggta
      541 ataaataatg atgaagcgca atattctggc agtgatcgtc cctgctctgt tagtagcagg
      601 tactgcaaac gctgcagaaa tctataacaa agatggcaac aaagtagatc tgtacggtaa
      661 agctgtcggt ctgcattatt
//
LOCUS       DAPOZO010000280          679 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPOZO010000280 DAPOZO010000000
VERSION     DAPOZO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.288"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            554..>679
                     /locus_tag="ROI21_004910"
     CDS             554..>679
                     /locus_tag="ROI21_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113701.1"
                     /translation="MSTPEKMIFHTRYRLTPVDLEARNSAVAHRLTELTANFTSAC"
BASE COUNT          184 a          153 c          185 g          157 t
ORIGIN      
        1 tgttgcgaaa agatgcttcc gcacgcacgc gcgataaagg agggaggact ttcgtgagac
       61 aaggggaaaa ctagcgttct gataattgca gagttgtttt cggaggtggt tacagtgagt
      121 cagcccgttc ctggcgagga ggagactgat atcaccgatg ggataaggct gtctgattgg
      181 tattataaat ataagaagtc atcagagagt aacgtaaagg atttgccaga gtaagctaag
      241 agagtaaatc tctccggtaa cagagcgctg tgaagcattc tgtatcagag tgcaaataca
      301 tgaacgctga tgcgtaaaat cagcagtgac acgccagctc cccctgtgcc cattcctgta
      361 taagacatga actgagcgta ttaatgctcc tgctctgaga ctgagccggt gagtgagggg
      421 ctgagaaatc cctcttgaca cagctctaaa ccagaaatac ttccccggtt gttgcggtgc
      481 gtccggatcc ccgataacgg aacactaagg ctgtattgcg ggacaccggg taagtggcag
      541 cctgtaatgt gttctgtcaa cgccagaaaa aatgattttt cacacgcgtt atagactcac
      601 tcctgtggac ctggaagccc gtaactctgc ggttgcccac cgactgacgg aactgaccgc
      661 aaactttacg agtgcgtgc
//
LOCUS       DAPOZO010000281          666 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.289, whole genome shotgun sequence.
ACCESSION   DAPOZO010000281 DAPOZO010000000
VERSION     DAPOZO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.289"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..545
                     /locus_tag="ROI21_004911"
     CDS             <1..545
                     /locus_tag="ROI21_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HDX5113702.1"
                     /translation="GRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLYEFKSKNIDDI
                     GIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERV
                     GDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTY
                     LKYNPKAKGNGVTIIRQGSN"
BASE COUNT          244 a           81 c          141 g          200 t
ORIGIN      
        1 aggggcggta tatcactcag gatccgattg ggctgaaggg gggatggaat ttttatcagt
       61 atccgttgaa tccgatctca aatatagatc cattaggatt atatgaattt aaatcaaaaa
      121 atatagatga tattggaata tttgcattgg caatgtgtaa tggagaatca attaacgaga
      181 ataaagaata tggtgggcta atatgtaaga agcaaggtga atatttcccc atgaatccga
      241 taagttcaaa tgataatgat agtgtagact tgcgaaatat aaaatgccct gaaggttcag
      301 agagagtagg cgattatcac actcacggtt tttactctga cgataaaggc aataaagtaa
      361 caaaagaaaa tgatgtttat gatagtctaa atttttcaag caaagattta acgaattctt
      421 atatgaatgg aatgggaaaa aaagaataca gtagttactt gggaacacca aataacacct
      481 atctaaaata taatcccaaa gctaaaggga atggagttac aattatcagg caagggagta
      541 attaaaatga aggtaataca atgttgttat ttccttgctt tagtctccag tcatttaatg
      601 gctggagtta tgagtgatga caatatattt ttaagtaatt cggatttagc tgctttatat
      661 tgtgtg
//
LOCUS       DAPOZO010000282          662 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPOZO010000282 DAPOZO010000000
VERSION     DAPOZO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.290"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>662
                     /locus_tag="ROI21_004912"
     rRNA            <1..>662
                     /locus_tag="ROI21_004912"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          185 a          153 c          189 g          135 t
ORIGIN      
        1 gacgaggcac tacggtgctg aagcaacaaa tgccctgctt ccaggaaaag cctctaagca
       61 tcaggtaaca tcaaatcgta ccccaaaccg acacaggtgg tcaggtagag aataccaagg
      121 cgcttgagag aactcgggtg aaggaactag gcaaaatggt gccgtaactt cgggagaagg
      181 cacgctgata tgtaggtgaa gcgacttgct cgtggagctg aaatcagtcg aagataccag
      241 ctggctgcaa ctgtttatta aaaacacagc actgtgcaaa cacgaaagtg gacgtatacg
      301 gtgtgacgcc tgcccggtgc cggaaggtta attgatgggg ttagcggtaa cgcgaagctc
      361 ttgatcgaag ccccggtaaa cggcggccgt aactataacg gtcctaaggt agcgaaattc
      421 cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga tggccaggct gtctccaccc
      481 gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt acccgcggca agacggaaag
      541 accccgtgaa cctttactat agcttgacac tgaacattga gccttgatgt gtaggatagg
      601 tgggaggctt tgaagtgtgg acgccagtct gcatggagcc gaccttgaaa taccaccctt
      661 ta
//
LOCUS       DAPOZO010000283          656 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPOZO010000283 DAPOZO010000000
VERSION     DAPOZO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.291"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..555
                     /locus_tag="ROI21_004913"
     CDS             <1..555
                     /locus_tag="ROI21_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDX5113703.1"
                     /translation="ETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIG
                     LVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLMVGLGYDVKVGNNVTFTVGKT
                     KKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINW
                     NCAASKSPPKTPDEKQDTPDMREY"
BASE COUNT          191 a          134 c          186 g          145 t
ORIGIN      
        1 gagaccattg gcaacaatca gatgattgcg gttaccaaca atcagataca gacggtgggc
       61 gttaaccaga tagagacggt gggcagtaac cagatcatta aagtgggctc tgttcaggtg
      121 gaaacgattg gacttgttcg tgcgctgact gtgggcgtgg cgtatcagac gacggtaggt
      181 ggcattatga acacttcggt ggccctgatg cagtcctcgc agatcggttt gcataaatcg
      241 ttgatggtcg ggctgggtta tgacgtcaaa gtcggaaata acgttacctt caccgttggt
      301 aaaacgaaaa aggatgatac cgggcagacc gcgatttact ccgccggtga gcatctggaa
      361 ctctgctgtg gtaaggcaag gctggtgctg actaaggacg gacaaatttt tctcaacggc
      421 acaaaaattc atttgcaggg taaggagcag gttaatggtg actcactgtt gattaactgg
      481 aactgtgcag cctcgaaatc cccaccgaag acccctgatg aaaagcagga tacgccggat
      541 atgagagagt actgatcccg tacctgttcg gtattcagta acaaacaagc attaaaaata
      601 aacagaagaa ggaattgaat catgagcgga aaaccagcgg cgcgtcaggg agatat
//
LOCUS       DAPOZO010000284          647 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPOZO010000284 DAPOZO010000000
VERSION     DAPOZO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.292"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>647
                     /locus_tag="ROI21_004914"
     CDS             <1..>647
                     /locus_tag="ROI21_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDX5113704.1"
                     /translation="LEGDLLRTDVAGMSVTAGIYGAAGHSSVDVKDDDGSRAGTVRDD
                     AGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGRGWLGSLETGLPFSI
                     TDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTS
                     SRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGT"
BASE COUNT          128 a          182 c          204 g          133 t
ORIGIN      
        1 ctggagggtg acctgctgag aacagatgtt gccggtatgt ctgtgaccgc agggatatat
       61 ggtgctgcag gccattcttc cgttgatgtt aaggatgatg acggctcccg tgccggcacg
      121 gtccgggatg atgccggcag cctgggcgga tatatgaacc tgacacacac ctcctccggc
      181 ctgtgggctg acatagtggc acagggaacc cgccacagta tgaaagcgtc atcgggcaat
      241 aacgacttcc gcgcacgggg ccggggctgg ctgggctcac tggaaaccgg tctgcccttc
      301 agtatcactg acaacctgat gctggagcca cggctgcagt acacctggca ggggctttcg
      361 ctggatgacg gtaaggacaa cgccggttat gtgaagttcg ggcatggcag tgcacaacat
      421 gtgcgtgccg gtttccgtct gggcagccac aacgatatga cctttggtga aggcacctca
      481 tcccgtgccc ccctgcgtga cagtgcaaaa cacagtgtgc gtgaactgcc ggtgaactgg
      541 tgggtacagc cttccgttat ccgcaccttc agctcccggg gtgatatgcg tgtggggacc
      601 tccactgcag gcagcgggat gacgttctct ccctcacaga atggcac
//
LOCUS       DAPOZO010000285          599 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPOZO010000285 DAPOZO010000000
VERSION     DAPOZO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.293"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>599
                     /locus_tag="ROI21_004915"
     CDS             <1..>599
                     /locus_tag="ROI21_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDX5113705.1"
                     /translation="DAVETLRANGFITAGRTDETTSEFFPLSEDAISRALRNYGLHPE
                     QLDAPAPHTEVASLHPNHVWQIDASLCTLYYLSNGHKGLQVMDSAKFYKNKPANLARI
                     ASDRVWSYEITDHASGWIYVEYVTGAESGENLCSVLINAMQERGGADVLHGVPKILYL
                     DPGSANTAGMTKNMCRSLGIDLIAHKPHNARATGQVEKAR"
BASE COUNT          144 a          174 c          168 g          113 t
ORIGIN      
        1 gatgcagtgg aaaccctgcg ggctaacggc tttatcaccg caggcagaac agatgaaacc
       61 accagtgagt ttttcccgtt gtctgaagat gccatcagcc gtgctctgcg taactatggc
      121 ctgcacccgg aacaactgga tgcccctgca ccacataccg aagtggccag tctgcatccc
      181 aatcatgtct ggcagattga cgcctcactc tgcacgcttt actacctgag caatggacat
      241 aaagggctgc aggtgatgga cagcgcgaag ttctacaaga acaagcccgc taaccttgcc
      301 cgtatcgcca gtgaccgcgt gtggagttac gagattaccg accatgccag cggctggatt
      361 tacgttgagt atgtgacggg cgcggaatca ggtgagaacc tgtgttctgt gcttatcaac
      421 gccatgcagg agcgtggcgg cgcagacgtg ctgcacggcg tgccgaaaat actctatctc
      481 gaccccggct cggcaaacac cgcgggtatg acgaaaaaca tgtgccgctc actgggcatc
      541 gacctgatag cgcacaagcc gcataacgcc cgcgccaccg gacaggtgga aaaggcgcg
//
LOCUS       DAPOZO010000286          599 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.294, whole genome shotgun sequence.
ACCESSION   DAPOZO010000286 DAPOZO010000000
VERSION     DAPOZO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.294"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>599
                     /locus_tag="ROI21_004916"
     CDS             <1..>599
                     /locus_tag="ROI21_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDX5113706.1"
                     /translation="DAVETLRANGFISAGRTDETTGEFFPLSEDAISRALRNYGLHPE
                     QLDAPAPHTEVASLHPNHVWQIDASLCTLYYLSNGHKGLQVMDSAKFYKNKPANLARI
                     ASDRVWSYEITDHASGWIYVEYVTGAESGENLCSVLINAMQERGGADVLHGVPKILYL
                     DPGSANTAGMTKNMCRSLGIDLIAHKPHNARATGQVEKAR"
BASE COUNT          140 a          174 c          170 g          115 t
ORIGIN      
        1 gatgcagtgg aaaccctgcg ggctaacggc tttatctccg caggcagaac agatgaaacc
       61 accggtgagt ttttcccgtt gtccgaagat gccatcagcc gtgccctgcg taactatggc
      121 ctgcacccgg aacaactgga tgcccctgcg ccgcataccg aagtggccag tctgcatccc
      181 aatcatgtct ggcagattga tgcctcgctc tgcacgcttt actacctgag caatggacac
      241 aaagggctgc aggtgatgga cagcgcgaaa ttctacaaga acaagcccgc taaccttgcc
      301 cgtatcgcca gtgaccgcgt gtggagttat gagattaccg accatgccag cggctggatt
      361 tacgttgagt atgtgacggg tgcggaatcc ggtgagaacc tgtgttctgt gcttatcaac
      421 gccatgcagg agcgtggcgg cgcagacgtg ctgcacggcg tgccaaaaat actctatctc
      481 gaccccggct cggcaaacac cgcgggtatg acgaaaaaca tgtgccgctc actgggcatt
      541 gacctgatag cgcacaagcc gcataacgcc cgcgccaccg gacaggtgga aaaggcgcg
//
LOCUS       DAPOZO010000287          573 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPOZO010000287 DAPOZO010000000
VERSION     DAPOZO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.295"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(1..573)
                     /locus_tag="ROI21_004917"
                     /pseudo
     CDS             complement(1..573)
                     /locus_tag="ROI21_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          159 a          136 c          101 g          177 t
ORIGIN      
        1 catgctctgg attatttaat tctttcagcg gaaacttttc ctcaaaggct ttatttagcc
       61 gcccctggtt tccttttaca gcgaataaat aatcaccgcc ctgtttttgt atcttctctg
      121 cgatatcttt ctggcaaccc atcgcatcag ttgtgataat ttttccttta atatccaaca
      181 tgttaagaag ttcagggata gctgtaattt cattggattt ctcatccgtc ctgatctgtc
      241 cgatgaccag actgtgcatt gttgagaacg aactaatgac atgaatcgct cccctgcggc
      301 gactctagtc ataagagtgc cggagcgttt ttccatcaat tgcaatgacg cctttatcat
      361 ctgaagaatg gcagtcacgc atccagttaa taaagcactc gtgaaatttt gcaggactga
      421 tacaggatac aactctggca atggtatcgt gaacaggaat accattttca aaatcaccat
      481 attgcttcaa aaaatcgaga tgtgtttccc caaaatcctc tatatcttcc cagccttctg
      541 caccagaaat aacggcacaa atagtcaaca gta
//
LOCUS       DAPOZO010000288          570 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPOZO010000288 DAPOZO010000000
VERSION     DAPOZO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.296"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..351
                     /locus_tag="ROI21_004918"
     CDS             <1..351
                     /locus_tag="ROI21_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDX5113707.1"
                     /translation="HGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQH
                     PRKNKTAINIEYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANL
                     FRADQMIRQWERSH"
BASE COUNT          178 a          139 c          149 g          104 t
ORIGIN      
        1 catggagagg agcaatttgt ctcagccgat gccggctacc aaggggcgcc acagcgcgag
       61 gagctggccg aggtggatgt ggactggctg atcgccgagc gccccggcaa ggtaagaacc
      121 ttgaaacagc atccacgcaa gaacaaaacg gccatcaaca tcgaatacat gaaagccagc
      181 atccgggcca aggtggagca cccatttcgc atcatcaagc gacagttcgg cttcgtgaaa
      241 gccagataca aggggttgct gaaaaacgat aaccaactgg cgatgttatt cacgctggcc
      301 aacctgtttc gggcggacca aatgatacgt cagtgggaga gatctcacta aaaactgggg
      361 ataacgcctt aaatggcgaa gaaacggtct aaataggctg attcaaggca tttacgggag
      421 aaaaaatcgg ctcaaacatg aagaaatgaa atgactgagt cagccgagaa gaatttcccc
      481 gcttattcgc accttcccta gaacaacaat gcctcgattg gtttaaagag ctaggttacc
      541 aataccaaaa tggctatgac atcgcaccag
//
LOCUS       DAPOZO010000289          559 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPOZO010000289 DAPOZO010000000
VERSION     DAPOZO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.297"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT          184 a           87 c          120 g          168 t
ORIGIN      
        1 aaaaaaccgg atgcgaggca tccggttgaa ataggggtaa acagacattc agaaatgaat
       61 gacggtaata aataaagtta atgatgatag cgggagttat tctagttgcg agtgaaggtt
      121 ttgttttgac attcagtgct gtcaaatact taagaataag ttattgattt taaccttgaa
      181 ttattattgc ttgatgttag gtgcttattt cgccattccg caataatctt aaaaagttcc
      241 cttgaattta cattttgaaa catctatagc gataaatgaa acatcttaaa agttttagta
      301 tcatattcgt gttggattat tctgcatttt tggggagaat ggacctgccg actgattaat
      361 gagggttaat cagtatgcag tggcataaaa aagcaaataa aggcatataa cagagggtta
      421 ataacatgaa agttaaagta ctgtccctcc tggtcccagc tctgctggta gcaggcgcag
      481 caaacgctgc tgaaatttac aacaaagacg gcaacaaatt agatctgtac ggtaaagttg
      541 acggcctgca ctatttctc
//
LOCUS       DAPOZO010000290          556 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPOZO010000290 DAPOZO010000000
VERSION     DAPOZO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.298"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>556
                     /locus_tag="ROI21_004919"
     CDS             <1..>556
                     /locus_tag="ROI21_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDX5113708.1"
                     /translation="LATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRMAAE
                     EGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFLYSAQIR
                     PSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFACWQMDG
                     WRNNAEVARGTSRSPEIWPGRRIAL"
BASE COUNT          129 a          157 c          163 g          107 t
ORIGIN      
        1 tactcgcgac gatcctgaaa gaaaacgggg tgaccgagtg gagtccgctg ttcagcgagc
       61 cgcatccttc ccgtgagttt tgtgtccagt acggcgaaac cgattacgat ttcctgtgcc
      121 ggatggcggc ggaggaaggc atcttctttt atgaggagca cgcgcaaaaa agtaccgacc
      181 agagcctggt cctgtgcgac accgtgcgtt atctgccgga gtcctttgag atcccctgga
      241 atccgaacac ccgtaccgag gtgagcaccc tatgcatcag ccagttcctc tacagcgcac
      301 aaatccgccc atcttccgtg gtgaccaaag actacacctt taaacgaccc ggctgggcag
      361 ggcgttttga tcaggaaggc cagcaccagg attaccagcg cacacagtat gaagtgtatg
      421 actaccccgg acgtttcaag ggtgcccacg ggcagaactt tgcctgctgg cagatggatg
      481 gctggcgaaa caacgcagaa gtggcgcgcg gaacaagccg ttcgccggag atatggccgg
      541 gacggcgaat tgcgct
//
LOCUS       DAPOZO010000291          549 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPOZO010000291 DAPOZO010000000
VERSION     DAPOZO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.299"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(301..>549)
                     /locus_tag="ROI21_004920"
     CDS             complement(301..>549)
                     /locus_tag="ROI21_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113709.1"
                     /translation="KILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVMCDED
                     YEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          139 a          176 c          120 g          114 t
ORIGIN      
        1 gtcagattct gaccagacgg ttcaggctgt acgcttacca ctattttatc cctgttgaat
       61 ttggttaagc cggtgcagac gcgccgggaa acgcacacct gaaccggtac aataaaatac
      121 aatacaaccc cctgttactg gagatacgaa tggaatgacg aggcagcctg tcagtcacag
      181 ggggctgttg gcataaaatc aatggaaagg ccaccaacac cggaattaag ctccacagtc
      241 cccgcactgg caatccattc ccgggaaagg catcaccgca aactgctccg gaggcaacca
      301 ttacagcctc tgtaacggat tatcagccct ggcatcttcc gacggcacct cctgccgccc
      361 ccacgccctg ctgtataccc cggtaccgca gcgtgggcag cacttttccc cctcataatc
      421 ttcgtcacac atcacacaaa accactttct caccttcacc gtataatccg gaaccgtcat
      481 gccctgcaga tgcaaacgac gggcctcttt ctctgccggg tcaagccagg tacatcgcag
      541 caggatttt
//
LOCUS       DAPOZO010000292          548 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPOZO010000292 DAPOZO010000000
VERSION     DAPOZO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.300"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>548
                     /locus_tag="ROI21_004921"
     CDS             <1..>548
                     /locus_tag="ROI21_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDX5113710.1"
                     /translation="QGNKLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYTSMLTQA
                     MDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRL
                     EGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSS
                     GLWADIVAQGTRHSMKASSDNN"
BASE COUNT          121 a          147 c          167 g          113 t
ORIGIN      
        1 tccaggggaa taagctgcag gccggtgcct ttaactactc cctcaaccgg gacagtgatg
       61 agagctggta tctgcgcagt gaaaatgctt atcgtgctga agtccccctg tatacatcca
      121 tgctgacaca ggcaatggac tatgaccgga ttctggcagg ctcccgcagc catcagaccg
      181 gtgtaaacgg tgaaaataac agcgtccgtc tcagcattca gggcggtcat ctcggtcacg
      241 ataacaacgg cggtattgcc cgtggagcca cgccggaaag cagtggcagc tatggcttcg
      301 tccgtctgga gggtgacctg ctgagaacag aggttgccgg tatgtctctg acgacagggg
      361 tgtatggtgc cgcaggccat tcttccgttg atgttaagga tgatgacggt tcccgcgccg
      421 gcacggtccg ggatgatgcc ggcagtctgg gcggatacct gaatctggta cacacgtcct
      481 ccggcctgtg ggctgacatt gtggcacagg gaacccgcca cagcatgaaa gcgtcatcgg
      541 acaataac
//
LOCUS       DAPOZO010000293          530 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.301, whole genome shotgun sequence.
ACCESSION   DAPOZO010000293 DAPOZO010000000
VERSION     DAPOZO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.301"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>530
                     /locus_tag="ROI21_004922"
     CDS             <1..>530
                     /locus_tag="ROI21_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDX5113711.1"
                     /translation="EFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEVTWYGWDGDHLTTVQTDSTRIQTVYEPGSFTPLIRIETDNGEREKTQRRSLAEK
                     LQQEGSEDGHGVVFPAELVRLLDRLEGEIRADRVSSESRQWLAQCGLTVERLATQIEP
                     VYLPERKIHLYHCDHRG"
BASE COUNT          122 a          140 c          194 g           74 t
ORIGIN      
        1 gagttctaca cgcggataca gcatggcgag ccactggtcg agagccgcta cctctacgac
       61 ccgctgggac ggcgaatggc gaaacgggta tggcggcggg agcgtgacct gacggggtgg
      121 atgtcgctgt cgcgtaaacc ggaggtgacg tggtacggct gggacggcga ccacctgacg
      181 acggtgcaga ccgacagcac acgtatccag acggtatacg agccgggaag cttcacaccg
      241 ctcatccgca tcgaaacaga caacggcgag cgggagaaaa cgcagcggcg gagcctggcg
      301 gagaagctcc agcaggaagg gagcgaggac ggacacggcg tggtgttccc ggctgaactg
      361 gtacggctgc tggacagact ggagggggaa atccgggcag accgcgtgag cagtgaaagc
      421 cgccagtggc tggcacagtg cgggctgacg gtggagcggc tggcgacaca gatagagccg
      481 gtttatttac ctgagcgaaa aattcatctt taccactgtg accaccgggg
//
LOCUS       DAPOZO010000294          530 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.302, whole genome shotgun sequence.
ACCESSION   DAPOZO010000294 DAPOZO010000000
VERSION     DAPOZO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.302"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>530
                     /locus_tag="ROI21_004923"
     CDS             <1..>530
                     /locus_tag="ROI21_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDX5113712.1"
                     /translation="EFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRVRDLTGWMSLS
                     RKPEVTWYGWDGDRLTTIQTQQSRIQTVYEPGSFTPLIRIETDNGEREKTQRHSLAEK
                     LQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEP
                     EYTPARKVHLYHCDHRG"
BASE COUNT          126 a          141 c          195 g           68 t
ORIGIN      
        1 gagttctaca cgcggataca gcatggcgag ccgcaggtgg agagccgcta cctctacgac
       61 ccgctgggcc gccggacggg taaacgggtg tggcggcggg tacgtgacct gacggggtgg
      121 atgtcgctgt cgcgtaaacc ggaggtaacg tggtacggct gggacggtga caggctgacg
      181 acgatacaga cacaacagag ccggatccag acggtatacg agccgggaag cttcacacca
      241 ctcatccgca tcgaaacaga caacggcgag cgggagaaaa cgcagcgcca cagcctggcg
      301 gagaagctgc agcaggaagg gagcgaggac gggcacggcg tggtgttccc ggctgaactg
      361 gtacggctgc tggacagact ggaggaagaa atccgggcag accgcgtgag cagtgaaagc
      421 cgggcgtggc ttgcgcagtg cgggctgacg gtagagcaac tggccagaca ggtggagccg
      481 gaatacacac cggcgcgaaa agttcatctt taccactgcg accaccgggg
//
LOCUS       DAPOZO010000295          524 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPOZO010000295 DAPOZO010000000
VERSION     DAPOZO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.303"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>524
                     /locus_tag="ROI21_004924"
     CDS             <1..>524
                     /locus_tag="ROI21_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDX5113713.1"
                     /translation="MTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAP
                     AVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHD
                     VHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKM
                     KTLFFGSGHGGERGA"
BASE COUNT          121 a          134 c          168 g          101 t
ORIGIN      
        1 atgactgacg gcaaactcca tgccgatgat accccggtcc aggtactgct gccgggtaat
       61 aagaagacga agaccgggcg gttgtgggcg tatgttcgtg atgaccgcaa tgccgggtca
      121 gcgttggcac ctgcagtgtg gttcgcttac agcccggaca gaaaaggcat ccatccgcag
      181 actcatcttg cttgcttcag cggtgtgctg caagcggatg cgtacgccgg gttcaacgag
      241 ctgtatcgca atggtgggat aacggaagct gcctgctggg ctcatgcccg ccgaaagatc
      301 cacgatgtgc acgtccgcat cccgtcagca ctgacggaag aagccctaga gcagatcggt
      361 cagttgtacg ccatagaggc ggatataagg ggaatgccgg cagagcagcg gcttgctgaa
      421 cgtcagcgaa aaacgaaacc gctgttgaaa tccctggaaa gctggttgcg tgaaaagatg
      481 aagaccctgt tcttcggctc tggccatggt ggtgagcggg gagc
//
LOCUS       DAPOZO010000296          500 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.304, whole genome shotgun sequence.
ACCESSION   DAPOZO010000296 DAPOZO010000000
VERSION     DAPOZO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.304"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>500
                     /locus_tag="ROI21_004925"
     CDS             <1..>500
                     /locus_tag="ROI21_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDX5113714.1"
                     /translation="LSSLPYSLSRHILEHLRKLTSHEPVIGIMGKSGAGKSSLCNALF
                     QGEVTPVSDVHAGTREVKRFRLSGHGHSMVITDLPGVGESRERDAEYEALYRDILPEL
                     DLVLWLIKTDDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPA
                     QAQNIR"
BASE COUNT          105 a          137 c          146 g          112 t
ORIGIN      
        1 ctttcttccc tgccttactc gctttcccgt cacatcctgg aacatctgcg caaactcacc
       61 agtcacgaac ccgtgattgg cattatgggt aaaagcgggg ccggtaaatc ctcactctgt
      121 aatgcgctgt ttcaggggga ggtcacaccg gtcagtgatg ttcacgccgg cacccgggaa
      181 gtgaagcgtt tccgtctgag tggacacggt cacagcatgg ttatcactga cctgcccggg
      241 gtgggcgaga gccgggagcg ggatgcagag tatgaagctc tgtaccgtga cattctgccc
      301 gaactggacc tggtactgtg gctgattaaa accgatgacc gtgccctgtc tgtggatgag
      361 tatttctggc gacacatcct gcaccgggga catcagcagg tgctgtttgt ggtgacgcag
      421 gccgataaaa cggagccctg ccatgaatgg gatatggccg gtattcagcc ttctcccgca
      481 caggcacaga acattcgcga
//
LOCUS       DAPOZO010000297          488 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPOZO010000297 DAPOZO010000000
VERSION     DAPOZO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.305"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>488
                     /locus_tag="ROI21_004926"
     CDS             <1..>488
                     /locus_tag="ROI21_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HDX5113715.1"
                     /translation="GLYYNRNRYYNPLLGRYITQDPIGLAGGWSLYAYPLNPVNGIDP
                     LGLSPADVALMRKKEQLNHQRAWDILSDTYDDMKRLNLGGTDQFFHCMAFCRVSKLND
                     AGVSRSAKGLGYEKEIRDYGLNMFGMYGRKVKLSHSEMIEDNKKDLAVNEHGLTCPFT
                     QD"
BASE COUNT          153 a           70 c          127 g          138 t
ORIGIN      
        1 gggctgtatt ataaccggaa ccggtactac aatccgttgc tgggacgata tattactcag
       61 gacccgattg ggctggcggg gggatggagt ctgtatgcgt atccgctgaa tccggtgaat
      121 ggtattgatc cattagggtt aagtcctgca gatgtagcgt taatgcgaaa aaaagaacaa
      181 ctaaaccatc aaagagcatg ggatatatta tctgatactt atgatgatat gaagagatta
      241 aatttaggtg ggactgatca atttttccat tgtatggcat tttgtcgagt gtctaaatta
      301 aatgacgctg gtgttagccg atcagcgaaa gggctgggtt atgaaaaaga gattagagat
      361 tacgggttaa atatgttcgg tatgtacggc agaaaagtaa agttatccca ttctgagatg
      421 attgaagata ataaaaaaga cttggctgta aatgaacatg ggttgacatg tccatttaca
      481 caagattg
//
LOCUS       DAPOZO010000298          472 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPOZO010000298 DAPOZO010000000
VERSION     DAPOZO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.306"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>472
                     /locus_tag="ROI21_004927"
     rRNA            <1..>472
                     /locus_tag="ROI21_004927"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          118 a          113 c          142 g           99 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg ga
//
LOCUS       DAPOZO010000299          469 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPOZO010000299 DAPOZO010000000
VERSION     DAPOZO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.307"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            220..456
                     /gene="lpp"
                     /locus_tag="ROI21_004928"
     CDS             220..456
                     /gene="lpp"
                     /locus_tag="ROI21_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDX5113716.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT          142 a          106 c           92 g          129 t
ORIGIN      
        1 cggatttcac tatataatct cactttatct aagatgaatc cgatggaagc atcctgtttt
       61 ctctcaattt ttttatctaa aacccagcgt tcgatgcttc tttgagcgaa cgatcaaaaa
      121 taagtgcctt cccatcaaaa aaatattctc aacataaaaa actttgtgta atacttgtaa
      181 cgctacatgg agattaactc aatctagagg gtattaataa tgaaagctac taaactggta
      241 ctgggcgcgg taatcctggg ttctactctg ctggcaggtt gctccagcaa cgctaaaatc
      301 gatcagctgt cttctgacgt tcagactctg aacgctaaag ttgaccagct gagcaacgac
      361 gtgaacgcaa tgcgttccga cgttcaggct gctaaagatg acgcagctcg tgctaaccag
      421 cgtctggaca acatggctac taaataccgc aagtaatagt acctgtgaa
//
LOCUS       DAPOZO010000300          466 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPOZO010000300 DAPOZO010000000
VERSION     DAPOZO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.308"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>466
                     /gene="tssI"
                     /locus_tag="ROI21_004929"
     CDS             <1..>466
                     /gene="tssI"
                     /locus_tag="ROI21_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDX5113717.1"
                     /translation="LNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDE
                     HGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQ
                     PIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVY"
BASE COUNT          119 a          123 c          142 g           82 t
ORIGIN      
        1 cgctgaataa ccactttgcg gtaataccgg cagacagaac atggcggcca cagccgttgc
       61 tgaaaccgct ggtggacggc ccgcagagcg ccgtcgtgac gggaccggca ggagaagaaa
      121 tcttctgtga tgaacatggc cgcgtgcggg tgaaatttaa ctgggaccgc tataacccgt
      181 caaaccagga cagttcatgc tggatccgtg tggcacaggc gtgggcaggc accggctttg
      241 gcaacctggc gataccgcgt gtgggtcagg aagtcattgt ggacttcctc aacggcgatc
      301 cggaccagcc gattattatg gggcgtacct accaccagga aaaccgcacc cccggcagcc
      361 tgccgggaac aaagacgcag atgaccattc gttcgaaaac ctataagggc agcgggttta
      421 atgaactgaa gtttgacgat gcgacaggga aagaacaggt ctacat
//
LOCUS       DAPOZO010000301          433 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.309, whole genome shotgun sequence.
ACCESSION   DAPOZO010000301 DAPOZO010000000
VERSION     DAPOZO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.309"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..306
                     /locus_tag="ROI21_004930"
     CDS             <1..306
                     /locus_tag="ROI21_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5113718.1"
                     /translation="RGEFIRGWDGGRKVDTGRALLSFQEGTIVSGFDDNDTGDISSLG
                     STQYGFGDALTSNQLGVLNGKKWIFDASSKGAQRYDWWAYVSARPRNIAFNYIVRAA"
     gene            complement(313..>433)
                     /locus_tag="ROI21_004931"
     CDS             complement(313..>433)
                     /locus_tag="ROI21_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDX5113719.1"
                     /translation="PEETAKLNAVLDYIDELNALDISKAPEISWPEAPLALAG"
BASE COUNT           93 a           99 c          119 g          122 t
ORIGIN      
        1 cgcggtgagt ttatccgtgg atgggacggt ggtcgcaagg ttgataccgg gcgtgcgcta
       61 ctttcatttc aggaagggac tatcgtatcc ggttttgatg ataatgatac cggagatatc
      121 agttcgctcg gctctacaca atacggattt ggtgacgctt taacctctaa tcaactgggg
      181 gtcttaaatg gcaaaaagtg gatatttgat gcatcctcta aaggtgcaca aagatatgac
      241 tggtgggctt atgtatcagc tcgcccccgc aatatcgcat ttaactatat cgtgagggcg
      301 gcgtgatacc gttcagccgg caagcgccag tggcgcttcc ggccagctga tttcaggtgc
      361 cttgctgata tccagtgcgt tcagctcgtc gatataatcc agcacggcgt tcagttttgc
      421 cgtttcttct ggt
//
LOCUS       DAPOZO010000302          432 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.310, whole genome shotgun sequence.
ACCESSION   DAPOZO010000302 DAPOZO010000000
VERSION     DAPOZO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.310"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>432
                     /locus_tag="ROI21_004932"
     CDS             <1..>432
                     /locus_tag="ROI21_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HDX5113720.1"
                     /translation="APSDIRLQIRDDALVLNDNGGRSIHFEPLLPGGAVYSRSESMWL
                     VRGGKAAQPDGHTLARLWASLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGTEDVL
                     PAPLPPYRVLTGLADRFGQTLTYRREAAGDLAGEITGVTDGA"
BASE COUNT           71 a          130 c          161 g           70 t
ORIGIN      
        1 gcgccttctg atatccgctt acagatacgc gatgatgcac tggtactcaa cgacaacggc
       61 ggacggagca ttcactttga gccgctgctg ccgggggggg cggtgtacag ccgcagcgag
      121 tcaatgtggc tggttcgcgg tggtaaggca gcgcagccgg acggccacac gctggcacgg
      181 ctgtgggcgt cgctgccgcc ggatatccgg ttaagcccgc atctttacct ggcgaccaac
      241 agcgcacagg gaccgtggtg gatactgggg tggtcagagc gggtgccggg cactgaggac
      301 gtactgccag cgccgctgcc gccgtaccgg gtgcttaccg ggctggcgga ccgcttcggg
      361 cagacgctga cgtaccggcg tgaggccgcc ggtgacctgg ccggggaaat caccggcgtg
      421 acggacggtg cc
//
LOCUS       DAPOZO010000303          429 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPOZO010000303 DAPOZO010000000
VERSION     DAPOZO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.311"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>429)
                     /locus_tag="ROI21_004933"
     CDS             complement(<1..>429)
                     /locus_tag="ROI21_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDX5113721.1"
                     /translation="VVRTPEEERYFPCVNISGEPEEYFRMSPEDWLSAEMQGEIVALV
                     HSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHKFRCVPHLTGRRFEHGVTDCYTLF
                     RDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEATGLYQVP"
BASE COUNT           94 a          155 c          101 g           79 t
ORIGIN      
        1 cggcacctga tacagccctg tggcctccag attatccaga tagagattct gaccgttacg
       61 ccaccagtca tccccgcgat gaaaatccgg catctcaatt cccgccagat ggtaagcgtc
      121 ccggaacagc gtgtaacagt ccgttacccc gtgctcaaag cgccgcccgg tgagatgcgg
      181 cacacagcgg aatttatgaa tcgccccccg gcagaccagc caccacggca aatcactctg
      241 tacctgcagc cgccggtcgg cctcactcag ccagggcaga ccaccggggt ggctgtggac
      301 cagcgccaca atctctccct gcatttctgc actcagccag tcctccggcg acatccggaa
      361 atactcctcc ggctcaccgg agatattcac gcagggaaaa tatctttcct cctccggcgt
      421 tctcaccac
//
LOCUS       DAPOZO010000304          425 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPOZO010000304 DAPOZO010000000
VERSION     DAPOZO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.312"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(113..>425)
                     /locus_tag="ROI21_004934"
     CDS             complement(113..>425)
                     /locus_tag="ROI21_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113722.1"
                     /translation="LVEEDRVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQ
                     NALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEAFPPELP
                     A"
BASE COUNT          110 a          101 c          105 g          109 t
ORIGIN      
        1 gccagtaata atgcaggttt tgaacaggtg aatcatggta tttacctctc atgagtcatg
       61 acgatgacac tggcaaatca ggtgataaaa cctaaaagga gcagaacaca cgttatgccg
      121 ggagctccgg ggggaatgct tcttccagtc ggttaaccat attgccgaga tgggatgcat
      181 catattccat gacggggcgt tgcctgatga tactgaccac cagtggtttg attaacatat
      241 tggtcgcggc ccgttgttgt ataccggcgg cgaaaatgat ctccagagca ttctgatcga
      301 taacacgggc cagattctcg ttcagatttt caatctcatg tccggtcagc aggaaactgc
      361 cggcaagcca tgtcacctgc tcctgaatca ttgccagaat tttgacgcga tcttcttcaa
      421 ccagc
//
LOCUS       DAPOZO010000305          424 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPOZO010000305 DAPOZO010000000
VERSION     DAPOZO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.313"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..313
                     /locus_tag="ROI21_004935"
     CDS             <1..313
                     /locus_tag="ROI21_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113723.1"
                     /translation="LVEEDRVKILAMIQEQMTWLAGSFLQTGHEIENLNANLARVIDQ
                     NALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPVMEYDASHLGNMVNRLEETLPPELP
                     A"
BASE COUNT          114 a          107 c           98 g          105 t
ORIGIN      
        1 gctggttgaa gaagatcgcg tcaaaattct ggcaatgatt caggagcaga tgacatggct
       61 tgccggcagt ttcctgcaga ccggacatga gattgaaaat ctgaacgcga atctggcccg
      121 tgttatcgat cagaatgctc tggagatcat tttcgccgcc ggtatacaac aacgggccgc
      181 gaccaacatg ttaatcaaac cactggtggt cagtatcatc aggcaacgcc ccgtcatgga
      241 atatgatgca tcccatctcg gcaatatggt taaccgactg gaagaaacat tacccccgga
      301 gttaccggca taacggctat tctgcgcctt ttacgtttta tcacctgatt tgtcagtgtc
      361 atcgtcatga ctcagagagg taaataccat gattcacctg ttcaaaacct gcatgattac
      421 cgcc
//
LOCUS       DAPOZO010000306          420 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.314, whole genome shotgun sequence.
ACCESSION   DAPOZO010000306 DAPOZO010000000
VERSION     DAPOZO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.314"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            53..>420
                     /locus_tag="ROI21_004936"
     CDS             53..>420
                     /locus_tag="ROI21_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113724.1"
                     /translation="MLVLKQQLKEARIPQAVVARAVDVSEATLAQIVNHNAWPRTSPG
                     EVRRRLASWLESQGIDTTKSFDAVQGAATPRTAGTTDKTSLSEEENMLLKKQVLFPAT
                     KKAFGLFRDPFADEAMQGSD"
BASE COUNT          106 a          105 c          126 g           83 t
ORIGIN      
        1 ccggcccgca gcagtgttat cagcattgtt aacggcaact gaggagggaa acatgctggt
       61 actgaagcag caactgaaag aggcccgtat tccacaggcg gtggtggcga gagctgtcga
      121 tgtttctgag gccacgctgg cccagattgt gaatcataac gcgtggcccc gcaccagccc
      181 cggagaagtg cgccggcgtc ttgcgtcctg gctggaaagt caggggattg atacaacgaa
      241 gagttttgat gctgtacagg gcgcggccac gccccgtaca gcgggtacca cagataaaac
      301 gagcctcagt gaggaagaga atatgttact caaaaaacag gtgttatttc cagcaaccaa
      361 aaaagcgttt ggtcttttcc gtgacccgtt cgccgacgaa gccatgcagg gttctgatga
//
LOCUS       DAPOZO010000307          416 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPOZO010000307 DAPOZO010000000
VERSION     DAPOZO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.315"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(192..>416)
                     /locus_tag="ROI21_004937"
     CDS             complement(192..>416)
                     /locus_tag="ROI21_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDX5113725.1"
                     /translation="LPDHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESI
                     CVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          111 a          100 c          118 g           87 t
ORIGIN      
        1 ctgaatctca tgtctgtgtg tatctgcgca cgttgctaac aggagttatt attagcatga
       61 cgatcgatat aagcgatcga taaaataggt atcgatcgtt ttattctatt atcaggctat
      121 tgcacggaca agagagacag taacgaaaac agcgcttttg tgagcaaggg agggcatcag
      181 tcacgacagg ttcagaagaa aatccagttc cataccgcgc gggcaacaga gaccactgtg
      241 tcacggacgg cacgcagaac cgtgcgtgca acagaggcaa cacagatgct ctcagctgtg
      301 tcaaatatcc ggtccaccgc accggtaaac tgttcacggg cctgagagcg gacagactcc
      361 gtgcgcagct cgtcctgcag tcgggtcatc agggggctgg cggcatggtc gggaag
//
LOCUS       DAPOZO010000308          415 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.316, whole genome shotgun sequence.
ACCESSION   DAPOZO010000308 DAPOZO010000000
VERSION     DAPOZO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.316"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>415
                     /locus_tag="ROI21_004938"
     CDS             <1..>415
                     /locus_tag="ROI21_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDX5113726.1"
                     /translation="VTPLPVLSADIVVHPGETVNGGTLVNHDNQFVSGTANGVTVSTG
                     LELGPDSDENTGGQWIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLA
                     VNTTLDNRGEQWVHGGGKAAGTIINQDGYQTIKHGG"
BASE COUNT          112 a           96 c          135 g           72 t
ORIGIN      
        1 gtcacgccac tcccggtgct gtctgctgac atcgttgtgc acccgggtga aacagtgaat
       61 ggcggaacac tggtaaacca tgacaaccag tttgtatccg gaacagctaa tggcgtgact
      121 gtcagtaccg ggcttgagct ggggccggac agtgacgaaa acaccggcgg gcaatggata
      181 aaagcgggtg gcacaggcag aaacaccact gtcaccgcaa atggtcgtca gattgtgcag
      241 gcaggaggaa ctgccagtga tacggttatt cgtgatggcg gagggcagag ccttaacgga
      301 ctggcggtga acaccacgct ggataacaga ggtgagcagt gggtacacgg gggagggaaa
      361 gctgccggta caattattaa ccaggatggt taccagacca taaaacatgg cggac
//
LOCUS       DAPOZO010000309          413 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPOZO010000309 DAPOZO010000000
VERSION     DAPOZO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.317"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..353)
                     /locus_tag="ROI21_004939"
     CDS             complement(<1..353)
                     /locus_tag="ROI21_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDX5113727.1"
                     /translation="MLIGYIRVSTNDQNTDLQRNALMCAGCEQIFEDKMSGTKSERPG
                     LKRALKCLKRGDTLVVWKLDRLGRRMKHLIALTEELRAKGVNFRSLTDSIDTSTPMGR
                     FFFHMMGALAEMEREL"
BASE COUNT          102 a          100 c           85 g          126 t
ORIGIN      
        1 agttcacgct ccatttctgc cagtgcaccc atcatatgaa aaaagaacct tcccatcgga
       61 gtgctggtat cgattgaatc cgtcagactg cgaaaattga caccttttgc gcgtagctct
      121 tctgtgagag caatgaggtg tttcattctt ctacccaacc gatccagctt ccagaccacc
      181 aatgtatccc ctcttttaag gcactttaaa gcgcgtttca ggcctggtcg ttccgacttg
      241 gttccgctca ttttgtcctc aaaaatctgt tcacatcctg cgcacattaa tgcattgcgt
      301 tgcaaatctg tgttttggtc atttgttgac acacgtatgt agccaatcag cattaaaaat
      361 actccctttt gagtggttaa agtactgaga taatgtgggg aggatttgag aga
//
LOCUS       DAPOZO010000310          412 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.318, whole genome shotgun sequence.
ACCESSION   DAPOZO010000310 DAPOZO010000000
VERSION     DAPOZO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.318"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>412
                     /locus_tag="ROI21_004940"
     CDS             <1..>412
                     /locus_tag="ROI21_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDX5113728.1"
                     /translation="VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTG
                     LELGPDSDDNTGGQQIARGGTARNTTVTANGLQDVMAGGSATDTVISAGGGQNLRGQA
                     YGTVLNGGEQWIHAGGSASGTVINQSGYQTIKHGG"
BASE COUNT           96 a          106 c          133 g           77 t
ORIGIN      
        1 gtcacgtcac tcccggtgct ggctgctgac atcgttgtgc acccgggtga aacagtgaat
       61 ggcggaacac tggtaaacca tgacaaccag tttgtatccg gaacagctga tggcgtgact
      121 gtcagtaccg ggcttgagct ggggccggac tctgatgaca acaccggcgg tcagcagata
      181 gcccggggtg gcactgccag aaacaccacg gtcaccgcaa acggtctgca ggatgtcatg
      241 gcaggtggca gtgccacgga tacggttatc agtgccggtg gaggacagaa tctgcgcggg
      301 caggcttacg gtactgtcct taacggtggt gaacaatgga tacatgcagg cggcagtgca
      361 tccggaacgg tgattaacca gtctggctac cagaccatta aacatggcgg gc
//
LOCUS       DAPOZO010000311          409 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPOZO010000311 DAPOZO010000000
VERSION     DAPOZO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.319"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..121
                     /locus_tag="ROI21_004941"
     CDS             <1..121
                     /locus_tag="ROI21_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDX5113729.1"
                     /translation="PEETAKLNAVLDYIDELNALDISTAPEISWPEAPLALAS"
     gene            complement(128..388)
                     /locus_tag="ROI21_004942"
     CDS             complement(128..388)
                     /locus_tag="ROI21_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113730.1"
                     /translation="MLSWQKGTLVGGHDDNDSSLDISYMSNGNNIDYGGDKVFAGNYR
                     SDYLWYAILGGTNSRAKAELNGAFFNITRPRNIAFNYIVRAA"
BASE COUNT          108 a          114 c           90 g           97 t
ORIGIN      
        1 accagaagaa acggcaaaac tgaacgccgt gctggattat atcgacgagc tgaacgcact
       61 ggatatcagc acggcacctg aaatcagctg gccggaagcg ccactggcac ttgccagctg
      121 aacggtatca cgccgccctc acgatatagt taaacgcgat attgcggggg cgggttatgt
      181 taaagaatgc tccgtttaat tcggcttttg cgcgactgtt tgttccaccc agaattgcgt
      241 accacaaata atcgctacgg taattccccg caaacacctt atcgccgcca tagtcaatat
      301 tatttccgtt actcatgtac gagatatcca gcgaagaatc gttatcgtca tgaccgccga
      361 ccagtgtacc tttctgccat gaaagcaact tacgcccctc gtcaatctt
//
LOCUS       DAPOZO010000312          395 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.320, whole genome shotgun sequence.
ACCESSION   DAPOZO010000312 DAPOZO010000000
VERSION     DAPOZO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.320"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(131..289)
                     /locus_tag="ROI21_004943"
     CDS             complement(131..289)
                     /locus_tag="ROI21_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDX5113731.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
BASE COUNT          111 a           92 c          101 g           91 t
ORIGIN      
        1 gatttactgg tgtaataaat gaaaaagcca cgtctcccgg agaagacgcg gcctgacaga
       61 tgaaatgaat gacgtttatt gtctgagaag cccttaactg gcgagctgag tattaagctg
      121 tgttccggca tcaccagcgc aactgacctt cagcattacg gataaccagc cgggaatatg
      181 ttccctggtc atcttcagta aacacattgc ggtaagctgt tttgacggca acagcctgtt
      241 cgcgtgagaa agggccttcg ggcaataaac gagatgtaca acccggcata tctgttgctc
      301 cctgaaagta aaaagccccg gtcatgatga ccggggcctg aaggagagtg acctgattat
      361 caaaaagtca cattcagcgt ggcctgaccg ttata
//
LOCUS       DAPOZO010000313          392 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPOZO010000313 DAPOZO010000000
VERSION     DAPOZO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.321"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            228..335
                     /locus_tag="ROI21_004944"
     CDS             228..335
                     /locus_tag="ROI21_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDX5113732.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIMNWLNKRK"
BASE COUNT           95 a           97 c          117 g           83 t
ORIGIN      
        1 tgatggattt cgacgaacaa aaatggccgt agaatcagcg gcaagccgga aaggttccgg
       61 tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt ggttgctgga gagagaaaac
      121 ccccgcacgt tgcaggtatg cacctgacaa caccacgggg gctaatcttg actctagacc
      181 actcaagaat agccgcgaaa cgttgtcatt acaacacagg cggctatatg acgctcgcag
      241 aactgggcat ggccttctgg catgatttag cggctccggt cattgctggc attcttgcca
      301 gtatgatcat gaactggctg aacaagcgga agtaacgtgt catgcgggcg tcaggctgcc
      361 gtaatggcaa tttgcgcccg gatcaggccg ca
//
LOCUS       DAPOZO010000314          388 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPOZO010000314 DAPOZO010000000
VERSION     DAPOZO010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.322"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>388
                     /locus_tag="ROI21_004945"
     rRNA            <1..>388
                     /locus_tag="ROI21_004945"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a           86 c          130 g           85 t
ORIGIN      
        1 gggtagtttg actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct
       61 aatcctggtc ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt
      121 gacggcgcga gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc
      181 catcgctcaa cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca
      241 tatcgacggc ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg
      301 tcccaagggt atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt
      361 gagacagttc ggtccctatc tgccgtgg
//
LOCUS       DAPOZO010000315          387 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPOZO010000315 DAPOZO010000000
VERSION     DAPOZO010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.323"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>387
                     /locus_tag="ROI21_004946"
     CDS             <1..>387
                     /locus_tag="ROI21_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDX5113733.1"
                     /translation="DIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRY
                     DRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSRHRLVHYTRTQYAEPLVESRYLYDPL
                     GRRVAKRVWRRERDLTGWMSLSRKPEV"
BASE COUNT           86 a          122 c          119 g           60 t
ORIGIN      
        1 gatatccgca tcccgtatgc cacggacccg gcaggcaacc gcctgcccga cccggagctg
       61 cacccggaca gcaccctcag catgtggccg gataaccgta tcgcccgtga cgcgcactat
      121 ctttaccggt atgaccgtca cggcaggctg acagagaaaa ccgacctcat cccggaaggg
      181 gttatccgca cggatgatga gcggactcac cggtatcatt acgacagccg gcaccggctg
      241 gtgcactaca cgcggacaca atatgcagag ccgctggtcg aaagtcgcta tctttacgac
      301 ccgctgggcc gcagggtggc aaaacgggta tggcggcgtg aacgggacct gacgggctgg
      361 atgtcgctgt cacggaaacc ggaagtg
//
LOCUS       DAPOZO010000316          387 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPOZO010000316 DAPOZO010000000
VERSION     DAPOZO010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.324"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>387
                     /locus_tag="ROI21_004947"
     CDS             <1..>387
                     /locus_tag="ROI21_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDX5113734.1"
                     /translation="DIRIPYATDPAGNRLPDPELHPDSPLSAWPDNRIARDAHYLYRY
                     DRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSRHRLVHYTRTQYAEPLVESRYLYDLL
                     GRRVAKRVWRRERDLTGWMSLSWKPEV"
BASE COUNT           83 a          123 c          121 g           60 t
ORIGIN      
        1 gatatccgca tcccgtatgc cacggacccg gcgggcaacc gcctgcccga cccggagctg
       61 cacccggaca gccccctcag cgcatggccg gataaccgta tcgcccgtga cgcgcactat
      121 ctttaccggt atgaccgtca cggcaggctg acggagaaaa ccgacctcat cccggaaggg
      181 gttatccgca cggatgatga gcggactcac cggtaccatt acgacagccg gcaccggctg
      241 gtgcactaca cgcggacaca atatgcagag ccgctggtcg aaagtcgcta tctttacgac
      301 ctgctgggcc gcagggtggc aaaacgggta tggcggcgtg aacgggacct gacgggctgg
      361 atgtcgctgt catggaaacc ggaagtg
//
LOCUS       DAPOZO010000317          368 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPOZO010000317 DAPOZO010000000
VERSION     DAPOZO010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.325"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT          111 a           84 c           90 g           83 t
ORIGIN      
        1 tttatctctt ccacaagtaa atattggcaa cccgagctgg aagcatcatc cagagcactc
       61 atgaaacgac tgtaacagct gttgaaaaac cggaatgcac tcttcattgc atacatttca
      121 gggatgaagt gaagatgaat tgagtcgtag tatccacacc aagccggcgt tatccggtga
      181 ggcgcaatgt tgcgggggct ctatccctgg tggcattggt tgctggaaag agaaaacccc
      241 cgcacgttga gaggtataac ctgacaacag aacgggggct aatcttgaac cagaacaatt
      301 caagaatagc cgcaaaatgt tgtcattaca acaaggcggc tatatgacgc tcacgcagtt
      361 tgccatga
//
LOCUS       DAPOZO010000318          367 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.326, whole genome shotgun sequence.
ACCESSION   DAPOZO010000318 DAPOZO010000000
VERSION     DAPOZO010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.326"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>367)
                     /locus_tag="ROI21_004948"
     CDS             complement(<1..>367)
                     /locus_tag="ROI21_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="activates the production of the major fimbrae
                     protein FimA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbriae biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDX5113735.1"
                     /translation="VKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHAVQMIL
                     SGYTFFPSETLNYIKSNKCSTNSSTITVLSNREVTILRYLVSGLSNKEIADKLLLSNK
                     TVSAHKSNIYGKLGLHSIVE"
BASE COUNT          114 a           70 c           63 g          120 t
ORIGIN      
        1 ctctacaatt gaatgcaaac ctagcttgcc ataaatatta gatttatgcg cactaactgt
       61 tttattgcta agtaataact tatcggcaat ttctttattg gataatccgc tgaccagata
      121 acgtaatatc gtcacttcac gattagatag cacagtgatc gttgaactat tcgtgctaca
      181 tttattgctt tttatatagt tgagcgtttc gctgggaaaa aaagtgtatc cggagaggat
      241 catctgaacg gcgtgaaaaa tatcattctg atcattgcat ttactgacaa aaccgttagc
      301 accagcttgt atcgctctgc cagcataaaa gcattctgat ttcgatgata aaaataatac
      361 tttcact
//
LOCUS       DAPOZO010000319          360 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.327, whole genome shotgun sequence.
ACCESSION   DAPOZO010000319 DAPOZO010000000
VERSION     DAPOZO010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.327"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(97..>360)
                     /locus_tag="ROI21_004949"
     CDS             complement(97..>360)
                     /locus_tag="ROI21_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HDX5113736.1"
                     /translation="NGKVKFANLTTGEVQWRNRPPSVSIRGADNVIELLRRLGLERFI
                     RVKEEINKDAILNEKEAVKNIPGISIKSDIEDFSIIPFEQDVQ"
BASE COUNT           94 a           87 c           70 g          109 t
ORIGIN      
        1 atttaattct cctctgtttc cggcgtgcag aagcccacgg cgctgacgcc ggaataaaaa
       61 agaaaatgtt tttattaaat aattaacgtg gtgtgtttac tgcacatcct gctcaaaagg
      121 aattattgaa aaatcctcaa tgtcgctttt aatggaaata ccgggaatat ttttcacggc
      181 ctctttttca ttcaggatag cgtctttatt tatttcctct tttacacgaa taaagcgctc
      241 aagccccaga cgtctcagta attcaataac attatccgcc ccacggatac tgactgatgg
      301 cggacggttt cgccactgca cctcgccggt ggtgaggtta gcgaacttca ccttcccgtt
//
LOCUS       DAPOZO010000320          359 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPOZO010000320 DAPOZO010000000
VERSION     DAPOZO010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.328"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..292
                     /locus_tag="ROI21_004950"
     CDS             <1..292
                     /locus_tag="ROI21_004950"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113737.1"
                     /translation="PGISISKLALENGINANLLFKWRQQWREGKLLLPSSESPQLLPV
                     TLDAAAEQPESLAEDPETLSISCEVTFRHGTLRFNGNVSEKLLTLLIQELKR"
BASE COUNT           94 a           99 c           87 g           79 t
ORIGIN      
        1 acccgggata tccatctcaa aacttgctct tgaaaatggc attaacgcca atctgttgtt
       61 caaatggcga caacaatggc gcgagggaaa gctgctatta ccttcttcag agagccccca
      121 gctacttcct gtgactctcg atgcagctgc cgaacagcca gaatcgctcg cagaggaccc
      181 ggaaaccctc agtatcagct gtgaggtaac gttccggcac gggacgctcc gcttcaatgg
      241 caatgtcagc gaaaagctcc tgactctgct gatacaggaa ctgaagcgat gatcccgtta
      301 ccttccggga ccaaaatttg gctggttgcc ggtatcaccg atatgagaaa tggcttcaa
//
LOCUS       DAPOZO010000321          353 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPOZO010000321 DAPOZO010000000
VERSION     DAPOZO010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.329"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            109..>353
                     /locus_tag="ROI21_004951"
     CDS             109..>353
                     /locus_tag="ROI21_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HDX5113738.1"
                     /translation="MAKSTKGAKRIKAAAALWVPGTREEVIEGIRLLGDAQRELVRAE
                     TEMNDTIGDITARYAPLTESLKKRMAELQSGIQTWCEA"
BASE COUNT          100 a           85 c          105 g           63 t
ORIGIN      
        1 catgaccaca ggggacattt ccgtcagggc gttttcgaac tgcacggctg tcgcgtcgtg
       61 tggccggaat ctttgtggta atcagcacaa caggaggagt cataaaatat ggcaaaaagt
      121 acaaaagggg caaaacgtat caaagccgca gcagcactct gggtgccggg gacacgcgaa
      181 gaggtcattg agggaatcag actgcttggt gacgcgcaac gtgaactggt cagggctgaa
      241 acagaaatga atgacaccat tggcgacatc actgcacgtt atgccccgct caccgagagc
      301 ctgaaaaaac gcatggccga actgcagtcc ggtatccaga catggtgtga ggc
//
LOCUS       DAPOZO010000322          353 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPOZO010000322 DAPOZO010000000
VERSION     DAPOZO010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.330"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            109..>353
                     /locus_tag="ROI21_004952"
     CDS             109..>353
                     /locus_tag="ROI21_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HDX5113739.1"
                     /translation="MAKSTKGAKRIKAAAALWVPGTREEVIEGIRLLGDAQRELVRAE
                     TEMNDAIGDITARYAPLTESLKKRMAELQSGIQTWCEA"
BASE COUNT          102 a           83 c          102 g           66 t
ORIGIN      
        1 catgaccaca ggggatattt ccgtcagggc gttttcgaac tgcacggctg tcgcgtcgtg
       61 tggccggaat ctttgtggta atcagcacaa ctggagaaat cataaaaaat ggcaaaaagt
      121 acaaaaggtg caaaacgtat caaggccgca gcagcactct gggtgccggg gacacgtgaa
      181 gaggtcattg agggaatcag actactcggt gacgcacaac gtgaactggt cagggctgaa
      241 acagaaatga atgacgccat tggcgatatc accgcacgtt atgccccgct caccgagagc
      301 ctgaaaaaac gcatggccga actgcagtcc ggtattcaga catggtgtga ggc
//
LOCUS       DAPOZO010000323          352 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPOZO010000323 DAPOZO010000000
VERSION     DAPOZO010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.331"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>352
                     /locus_tag="ROI21_004953"
     CDS             <1..>352
                     /locus_tag="ROI21_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDX5113740.1"
                     /translation="DGRRAVLRSQVLELHGISHGSAGARSIATMATRRGYQMGRWLAG
                     RLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGKRW
                     AYLAVVLDLFARKPV"
BASE COUNT           81 a           91 c          109 g           71 t
ORIGIN      
        1 cagacggcag acgggctgta ttacgcagtc aggtacttga gctacatggc atcagccacg
       61 gttcggccgg agcaagaagc atcgccacaa tggcaacccg gagaggctac cagatgggac
      121 gctggcttgc tggcaggctc atgaaagagc tggggctggt cagctgtcag cagccgactc
      181 accggtataa acgtggtggt catgaacatg ttgctatccc taactacctt gaaaggcagt
      241 tcgccgtgac cgagccaaat caggtgtggt gcggtgatgt gacctatatc tggacgggta
      301 agcgctgggc gtacctcgcc gttgttctcg acctgttcgc aagaaaacca gt
//
LOCUS       DAPOZO010000324          350 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.332, whole genome shotgun sequence.
ACCESSION   DAPOZO010000324 DAPOZO010000000
VERSION     DAPOZO010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.332"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>350)
                     /locus_tag="ROI21_004954"
     CDS             complement(<1..>350)
                     /locus_tag="ROI21_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDX5113741.1"
                     /translation="VSAVTATINSSSQSQNVTFIADVRTAKIADLVVIKDGSEADGST
                     ANTLRARVTDAFGNALAGQAVSVMAGNSATVTPTVTTQSDGTVEFSVTSQTAGTSTVT
                     ASINSSSLSRDVTF"
BASE COUNT           58 a          116 c           93 g           83 t
ORIGIN      
        1 ataaacgtca cgtcccggct caggctgctg ctgttgatac tggcggtgac tgtactggtt
       61 cctgccgtct ggctggtgac actgaactcc accgtaccgt ccgactgcgt ggtgaccgtc
      121 ggagtgaccg ttgcgctgtt gcctgccatc acgctgaccg cctgcccggc aagtgcattg
      181 ccgaacgcat cggtcaccct cgcccgcagc gtgttcgccg tcgaaccgtc cgcctctgaa
      241 ccatccttaa tgaccaccag atccgcgatc ttcgccgtcc tgacatcggc gataaatgtc
      301 acgttctgac tctgactact gctgttgatg gttgcagtga ctgcactgac
//
LOCUS       DAPOZO010000325          350 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.333, whole genome shotgun sequence.
ACCESSION   DAPOZO010000325 DAPOZO010000000
VERSION     DAPOZO010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.333"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..224
                     /locus_tag="ROI21_004955"
     CDS             <1..224
                     /locus_tag="ROI21_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDX5113742.1"
                     /translation="VLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            122..>350
                     /locus_tag="ROI21_004956"
     CDS             122..>350
                     /locus_tag="ROI21_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDX5113743.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSA"
BASE COUNT           76 a          100 c          102 g           72 t
ORIGIN      
        1 atgtgctgct gtgctgtttt ggttcatcgg tgccgaatca tgccgccatt tactgtggtg
       61 acagcgagct gctgcaccat attcctgaac aactgagcaa acgagagagg tacaccgaca
      121 aatggcagcg acgcacacac tccctctggc gtcaccgggc atggcacgca tctgccttta
      181 cggggattta caacgatttg gccgccgcat cgaccttcga gtgaaaacgg gggccgaagc
      241 catccgggcg ctggccatgc agatcccggc atttcgtcag aaactgagcg acggctggta
      301 ccaggtacgc attgccgggc gtgatgcagg tgaaaccgaa ttgtctgccc
//
LOCUS       DAPOZO010000326          350 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPOZO010000326 DAPOZO010000000
VERSION     DAPOZO010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.334"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>350
                     /locus_tag="ROI21_004957"
     CDS             <1..>350
                     /locus_tag="ROI21_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDX5113744.1"
                     /translation="FQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHSTIYRWVQ
                     RYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDFYLSSRR
                     NSKAAYRFLGKILN"
BASE COUNT           75 a           94 c           97 g           84 t
ORIGIN      
        1 tttcagcgtg acatcattct gtgggccgta cgctggtact gcaaatacgg catcagttac
       61 cgtgagctgc aggagatgct ggctgaacgc ggagtgaatg tcgatcactc cacgatttac
      121 cgctgggttc agcgttatgc gcctgaaatg gaaaaacggc tgcgctggta ctggcgtaac
      181 ccttccgatc tttgcccgtg gcacatggat gaaacctacg tgaaggtcaa tggccgctgg
      241 gcgtatctgt accgggccgt cgacagccgg ggccgcactg tcgattttta tctctcctcc
      301 cgtcgtaaca gcaaagctgc ataccggttt ctgggtaaaa tcctcaacaa
//
LOCUS       DAPOZO010000327          349 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPOZO010000327 DAPOZO010000000
VERSION     DAPOZO010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.335"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..318
                     /locus_tag="ROI21_004958"
     CDS             <1..318
                     /locus_tag="ROI21_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113745.1"
                     /translation="VDTGRKLLSAQGATLLRTAMLDYYNQDTTGTSGIVGMGFNNEDS
                     ITDLREGSFKMPDGTTFSDPVVAMSDNGMQATILTSIRSGYAKGITVRPRSIALNYIV
                     RAV"
BASE COUNT          100 a           73 c           94 g           82 t
ORIGIN      
        1 gttgatacag gacgtaagtt gctgtccgca caaggggcaa cgctgttaag aacagcaatg
       61 ctggattatt ataaccagga cactacggga acctcgggga tagtcggcat gggattcaac
      121 aatgaagatt ccattacaga ccttcgtgag ggcagcttta aaatgccgga cgggacaaca
      181 ttcagcgatc ctgtcgtggc aatgtcagac aatggtatgc aggctactat tctgacctct
      241 atcagaagcg ggtatgcgaa gggtatcact gtcagacccc gtagcattgc acttaattac
      301 attgtgaggg cagtttaatg agtaacactg cagttctgga tgaaaacgg
//
LOCUS       DAPOZO010000328          348 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPOZO010000328 DAPOZO010000000
VERSION     DAPOZO010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.336"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..196)
                     /gene="tnpB"
                     /locus_tag="ROI21_004959"
     CDS             complement(<1..196)
                     /gene="tnpB"
                     /locus_tag="ROI21_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDX5113746.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLC"
BASE COUNT           76 a           90 c           98 g           84 t
ORIGIN      
        1 gacacagtcc atcgccggta gaccagagga gctttacctg actgccatta cgcccacgga
       61 agataaaaac gtgacctgac atcggatcgt ctttcagcgt cgtctgcacc tttgccgcca
      121 ggccgttgaa gccgtttctc atatcggtga tgccagcgac cagccagatc tttgtccctg
      181 atggtaatgg gatcaacgtt tgagctcccg tatcagcagg ttcaggatat tttcgctgat
      241 ggcaccattc agacggagtg atccgtgccg gaacgtcact tcacagctga tattgagtga
      301 ttcaggtgtc gcagcagcta caggttgtga ccggggggag gttgtagg
//
LOCUS       DAPOZO010000329          348 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPOZO010000329 DAPOZO010000000
VERSION     DAPOZO010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.337"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(105..>348)
                     /locus_tag="ROI21_004960"
     rRNA            complement(105..>348)
                     /locus_tag="ROI21_004960"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           74 a          104 c           75 g           95 t
ORIGIN      
        1 ggccgccagg caaattctgt ttcatcagac cgcttctgcg ttctgattta atctgtatca
       61 ggctgaaaat cttctctcat ccgccaaaac atcttcggcg ttgtaaggtt aagcctcacg
      121 gttcattagt accggttagc tcaacgcatc gctgcgctta cacacccggc ctatcaacgt
      181 cgtcgtcttc aacgttcctt caggactctc aaggagtcag ggagaactca tctcggggca
      241 agtttcgtgc ttagatgctt tcagcactta tctcttccgc atttagctac cgggcagtgc
      301 cattggcatg acaacccgaa caccagtgat gcgtccactc cggtcctc
//
LOCUS       DAPOZO010000330          340 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPOZO010000330 DAPOZO010000000
VERSION     DAPOZO010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.338"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>340
                     /locus_tag="ROI21_004961"
     rRNA            <1..>340
                     /locus_tag="ROI21_004961"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           92 a           72 c          103 g           73 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
//
LOCUS       DAPOZO010000331          331 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPOZO010000331 DAPOZO010000000
VERSION     DAPOZO010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.339"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..59
                     /locus_tag="ROI21_004962"
     CDS             <1..59
                     /locus_tag="ROI21_004962"
                     /inference="COORDINATES: protein motif:HMM:NF015205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDX5113747.1"
                     /translation="RDYALQHPECSAIMRIID"
     gene            75..>331
                     /locus_tag="ROI21_004963"
     CDS             75..>331
                     /locus_tag="ROI21_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113748.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEV"
BASE COUNT           72 a          100 c           96 g           63 t
ORIGIN      
        1 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatt atcgactgaa
       61 tggaggcagg gacaatgcag cagctttcct ttctgcccgg agagatgacg cccggcgagc
      121 gcagtctcat tctgcgggcc ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgg
      181 ccttcacctc cacccgtgcg gcacgggaat ggctgattct gaacatggcg ggactggagc
      241 gggaagagtt ccgggtgctg tatctgaaca accagaatca gctgattgcc ggtgaaaccc
      301 tcttcaccgg caccatcaac cgcacggaag t
//
LOCUS       DAPOZO010000332          323 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.340, whole genome shotgun sequence.
ACCESSION   DAPOZO010000332 DAPOZO010000000
VERSION     DAPOZO010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.340"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            229..>323
                     /locus_tag="ROI21_004964"
     CDS             229..>323
                     /locus_tag="ROI21_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDX5113749.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNWL"
BASE COUNT           74 a           79 c           97 g           73 t
ORIGIN      
        1 tgatggattt cgacgaacaa aaatggccgt agaatcagcg gcaagccgga agggttccgg
       61 tgaggcgcaa tgttgcgggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatctagac
      181 cattcaagaa tagccgcgaa acgttgtcat tacaactcag gcggctatat gacgctcgca
      241 gagctgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
      301 agtctgatcg tgaactggct gaa
//
LOCUS       DAPOZO010000333          322 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPOZO010000333 DAPOZO010000000
VERSION     DAPOZO010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.341"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT           95 a           62 c           63 g          102 t
ORIGIN      
        1 ttattaaaat tccagttctt ctttttctga ttacagaagg caaagtggca attacgcata
       61 gtttcccgat aaagacgcga tagcgacatc ccgcataagg catttttctc tttatctttg
      121 tacggtactt catggaacag agtttttgac cttgcgaatc gtgatgtctg ttggggggca
      181 acttgctcac tgaagcgtga gactcgatta agcgcacgaa acacagaaat caaaaaaccc
      241 ggtcactttt ttacaaggta accgggtaaa aataattttt attttttaac tgttttgaga
      301 ctcatagaga tgtctcaaaa ct
//
LOCUS       DAPOZO010000334          320 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPOZO010000334 DAPOZO010000000
VERSION     DAPOZO010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.342"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT           97 a           66 c           54 g          103 t
ORIGIN      
        1 aatatgaatg gtgaatatgt ctggccctgg atattgccgt tttcagcggc aggcatgaaa
       61 catactggca tacagtatca gtagatattg cattagtgta tcctgcacac aagtaataat
      121 ttatccacca ataataacac tgttaatgtc cccttcccct ggttgtcagc caggggttat
      181 cttctgaata tttcttttga aaaggataac acagtaaatt atttttatga attatcccat
      241 ggactcatta acaccctttc ataatgcttt attgtcaaac acgttatggc tgacatcaaa
      301 aaaaaccgga tttcctctgc
//
LOCUS       DAPOZO010000335          320 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.343, whole genome shotgun sequence.
ACCESSION   DAPOZO010000335 DAPOZO010000000
VERSION     DAPOZO010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.343"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>320)
                     /locus_tag="ROI21_004965"
     CDS             complement(<1..>320)
                     /locus_tag="ROI21_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113750.1"
                     /translation="QAQRAEEARKQHTASLFSPDTPRPLSASAFPDTLPGTEYGPDRG
                     IRLSAVWLMHDPAYPESLPGAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDAQHPGRM
                     VAHR"
BASE COUNT           54 a           96 c          112 g           58 t
ORIGIN      
        1 taacggtgcg ccaccatccg gcccgggtgc tgcgcgtcat acgtgaaagc gcgcacctgc
       61 gtattgctgc ggtcatatac cgccagcagt tcaccggctt ccgtatacgt gtaccgcacc
      121 agtggcgcac cgggcaggct ctccgggtat gccgggtcgt gcatcagcca caccgccgaa
      181 agacggatgc ccctgtcggg gccgtattcg gtaccgggca gtgtgtcggg gaacgctgag
      241 gctgaaagag ggcggggggt gtcaggggaa aataaggaag cggtgtgctg tttacgggcc
      301 tcttccgccc gctgcgcctg
//
LOCUS       DAPOZO010000336          315 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPOZO010000336 DAPOZO010000000
VERSION     DAPOZO010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.344"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT           82 a           85 c           69 g           79 t
ORIGIN      
        1 caataatcag tgccagcagt atggtaacga tacggtctgt attccggtaa tagccgaagg
       61 ggtcgacaga accacaatac gggcaacaca ctgtattatg agtaatcgcg tttccacagt
      121 ccctgcactt ctttttgcgt atcaccatca ctccctcctc acagccttac ccgtaaccgg
      181 ttttttacat taaaaaaaca acgctgaaaa ttcaaccgtc agtccggaga cgaccgttcg
      241 ggttatcaca gagtgcctga gacagtgtcc tgccggaggt cacttcaggg actctgcgca
      301 ttttttacga cgtgg
//
LOCUS       DAPOZO010000337          312 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPOZO010000337 DAPOZO010000000
VERSION     DAPOZO010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.345"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(76..309)
                     /locus_tag="ROI21_004966"
     CDS             complement(76..309)
                     /locus_tag="ROI21_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDX5113751.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRIIR
                     NTEGQRRWRCRNSEPDAGKVLNTQLASDGLLRQ"
BASE COUNT           79 a           79 c           82 g           72 t
ORIGIN      
        1 gatttactgg tgtaataaat gaaaaagcca cgtctcccgg agaagacgcg gcctgatggg
       61 tgaaatgaat gacgtttatt gtctgagaag accgtcactg gcgagctgcg tattaagcac
      121 ctttccggca tcgggttcag aattccggca gcgccagcgt cgctgacctt ccgtgttgcg
      181 gataatccga cggaaatgca ttcccgggtc atcctggata aacacattgc ggtaagctgt
      241 tatgacagca accgcctgcc cgtatgagaa agatccttca gccaggacat actctgtgta
      301 acccggcata tc
//
LOCUS       DAPOZO010000338          311 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPOZO010000338 DAPOZO010000000
VERSION     DAPOZO010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.346"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..>311)
                     /locus_tag="ROI21_004967"
     CDS             complement(<1..>311)
                     /locus_tag="ROI21_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113752.1"
                     /translation="QAQRAEEARTSSLSSSDRSRPLSASAFPDTLPGTEYGPDRGIRL
                     SAVWLMHDPAYPENLPGAPLVRYTYTEAGELLAVYDRSGTQVRAFTYDPQHPGRMVGH
                     R"
BASE COUNT           56 a           97 c          106 g           52 t
ORIGIN      
        1 taacggtgcc ccaccatccg gcccgggtgc tgcgggtcat acgtgaaagc gcgcacctgt
       61 gtcccgctgc ggtcatatac cgccagcagt tcaccggctt ccgtatacgt gtaccgcacc
      121 agtggcgcac cgggcagatt ctccgggtat gccgggtcgt gcatcagcca caccgccgaa
      181 aggcggatac ccctgtcggg gccgtattcc gtaccgggca gtgtgtcggg gaacgctgag
      241 gctgagagag ggcgggaacg gtcagaagaa gatagcgaag aggtgcgggc ctcttccgca
      301 cgctgcgcct g
//
LOCUS       DAPOZO010000339          298 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPOZO010000339 DAPOZO010000000
VERSION     DAPOZO010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.347"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            16..>298
                     /locus_tag="ROI21_004968"
     CDS             16..>298
                     /locus_tag="ROI21_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I deoxyribonuclease HsdR"
                     /protein_id="HDX5113753.1"
                     /translation="MPERENYQQVTLQARLVSALETLNPNVPSDTLIEVAKIVATPQT
                     PVLIKNNKLFHQYLIEGVPVEYSVMEQEQWVTKHTHVRLIDFVLWSTKTG"
BASE COUNT           93 a           68 c           62 g           75 t
ORIGIN      
        1 gcaccagatg gtgatatgcc tgaacgtgaa aactatcagc aagtcacgct acaggcgcgt
       61 ttagtctctg ccttggaaac cttaaaccca aatgtgccca gtgacacctt aatcgaagta
      121 gcgaaaatcg ttgccacacc gcaaacgcct gtactgatta aaaacaacaa actctttcac
      181 caatatttaa tcgaaggtgt gcctgttgaa tactcggtaa tggagcaaga acaatgggtg
      241 accaagcata ctcatgttcg attaattgat tttgtgttgt ggtcaactaa aactggcc
//
LOCUS       DAPOZO010000340          290 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPOZO010000340 DAPOZO010000000
VERSION     DAPOZO010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.348"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..209
                     /locus_tag="ROI21_004969"
     CDS             <1..209
                     /locus_tag="ROI21_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HDX5113754.1"
                     /translation="TPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIH
                     SIDTDIKLNRALWVMAETLLESLR"
BASE COUNT           81 a           73 c           69 g           67 t
ORIGIN      
        1 tgacgccacg acgccgggag gattacggta aggacctgtg gagtgcttat cagaccattc
       61 aggagaatat gctgaaaggc ggaatttcag gtcgcagcgc taaaggaaaa cgtatccata
      121 cccgtgccat tcacagcatt gacactgaca ttaagctcaa ccgcgcattg tgggtgatgg
      181 cggaaaccct gctggagagc ctgcgctgat tcagccatta ccctgaaaac cacgcattta
      241 tttgtccttt aaggagtcat ttcatgaaaa ccatttcaca taattccacc
//
LOCUS       DAPOZO010000341          281 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPOZO010000341 DAPOZO010000000
VERSION     DAPOZO010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.349"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>281
                     /locus_tag="ROI21_004970"
     CDS             <1..>281
                     /locus_tag="ROI21_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113755.1"
                     /translation="ARGGTLAGTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSL
                     TGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDSTVTTDVIAQRGT"
BASE COUNT           75 a           90 c           75 g           41 t
ORIGIN      
        1 gccaggggcg gcacactggc gggcaccacc acgctgaata acggcgccat acttaccctt
       61 tccgggaaga cggtgaacaa cgataccctg accatccgtg aaggcgatgc actcctgcag
      121 ggaggctctc tcaccggtaa cggcagcgtg gaaaaatcag gaagtggcac actcactgtc
      181 agcaacacca cactcaccca gaaagccgtc aacctgaatg aaggcacgct gacgctgaac
      241 gacagtaccg tcaccacgga tgtcattgct cagcgcggta c
//
LOCUS       DAPOZO010000342          278 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPOZO010000342 DAPOZO010000000
VERSION     DAPOZO010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.350"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            24..100
                     /locus_tag="ROI21_004971"
     tRNA            24..100
                     /locus_tag="ROI21_004971"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ROI21_004972"
     tRNA            143..218
                     /locus_tag="ROI21_004972"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           63 c           72 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacc gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAPOZO010000343          278 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPOZO010000343 DAPOZO010000000
VERSION     DAPOZO010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.351"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT           68 a           71 c           75 g           64 t
ORIGIN      
        1 agccctgcca gcgcagaagc tgtgctgatt aatgctctga acaaaaggcg aagaatggca
       61 aatcctgcta tcagcaggat cagcgcgccg actatctgta acatagtcac tccgtgatga
      121 atatcatgtt attgttaatg ccagcgaatg tggcgttgaa tatccgttgc gccggtccgg
      181 gtcccgatca tgatgaccgg gaccacaaag aggcgatggt tctgtcagaa ggtcacattc
      241 agcgtggcct gaccgttata gccttcagcg ctgctgcc
//
LOCUS       DAPOZO010000344          278 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPOZO010000344 DAPOZO010000000
VERSION     DAPOZO010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.352"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            20..229
                     /locus_tag="ROI21_004973"
     CDS             20..229
                     /locus_tag="ROI21_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDX5113756.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRRW
                     RCRNSEADAGKQLNAWLASGGLLRQ"
BASE COUNT           59 a           74 c           81 g           64 t
ORIGIN      
        1 gggttacaca gagtatgtcc tggctgaagg atctttctca tacgggcagg cggttgctgt
       61 cataaccgct taccgcaatg tgtttatcca ggatgacccg ggaatgcatt tccgtcgggt
      121 tatccgcaac gcggaaggtc agcgacgctg gcgctgccgg aactctgaag ctgatgccgg
      181 aaaacagctt aatgcgtggc tcgccagtgg agggcttctc agacaataaa cctcattgat
      241 ttcatccatc aggccgcgtc ttctccggga gacgcggc
//
LOCUS       DAPOZO010000345          271 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPOZO010000345 DAPOZO010000000
VERSION     DAPOZO010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.353"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..167)
                     /locus_tag="ROI21_004974"
     CDS             complement(<1..167)
                     /locus_tag="ROI21_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113757.1"
                     /translation="MKSTTGINQQISKVQSAIMALKATNTDVQSITIRGNKPVIRVSR
                     SAHCMRMLEQG"
     gene            complement(170..>271)
                     /locus_tag="ROI21_004975"
     CDS             complement(170..>271)
                     /locus_tag="ROI21_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113758.1"
                     /translation="LYPLAVSNLVIAAMNLAAEIGVPQVNADVVKGV"
BASE COUNT           55 a           70 c           70 g           76 t
ORIGIN      
        1 tttccctgct caagcatgcg catgcaatgc gcactccggg aaacgcggat gacaggtttg
       61 ttacccctga tggtgatgct ttgtacatcc gtgttcgtcg ccttaagcgc cataatggct
      121 gactgcactt tgctgatttg ctggttgata cctgtggtgg atttcattat taaacccctt
      181 tgacaacgtc agcgttgacc tgtggaaccc cgatttcagc ggccagattc atggcggcta
      241 ttaccaggtt actgacggcc agcggataca g
//
LOCUS       DAPOZO010000346          261 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPOZO010000346 DAPOZO010000000
VERSION     DAPOZO010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.354"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>261
                     /locus_tag="ROI21_004976"
     CDS             <1..>261
                     /locus_tag="ROI21_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113759.1"
                     /translation="VVKNVTVSQSCGKWYISIQTENEVSTPVHPSASMVGLDAGVAKL
                     ATLSDGTVFGPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQK"
BASE COUNT           76 a           61 c           69 g           55 t
ORIGIN      
        1 gttgtgaaaa atgtcacggt cagccagtcc tgcggtaagt ggtacatcag tattcagact
       61 gaaaatgaag tatcaactcc ggttcaccct tcagcatcaa tggtcgggct ggatgctggc
      121 gtggctaaac tcgccacgct gtcagatggc acagtctttg ggcctgtaaa cagttttcag
      181 aaaaaccaga agacgctggc gagacttcag cggcagttaa gccgcaaggt caaattcagc
      241 aacaactggc agaagcagaa a
//
LOCUS       DAPOZO010000347          261 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPOZO010000347 DAPOZO010000000
VERSION     DAPOZO010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.355"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>261
                     /locus_tag="ROI21_004977"
     CDS             <1..>261
                     /locus_tag="ROI21_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113760.1"
                     /translation="VVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMVGLDAGVAKL
                     ATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQK"
BASE COUNT           76 a           63 c           67 g           55 t
ORIGIN      
        1 gttgtgaaaa atgtcactgt cagccagtcc tgcggtaagt ggtacatcag tattcagacc
       61 gaaagtgaag tatccactcc ggttcaccct tcagcatcaa tggtcgggct ggatgctggc
      121 gtggctaaac tcgccacgct gtcagatggc acagtctttg agcctgtaaa cagttttcag
      181 aaaaaccaga agacgctggc gagacttcag cgacagttaa gccgcaaggt caaattcagc
      241 aacaactggc agaagcagaa a
//
LOCUS       DAPOZO010000348          260 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPOZO010000348 DAPOZO010000000
VERSION     DAPOZO010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.356"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT           67 a           50 c           75 g           68 t
ORIGIN      
        1 agcgttcggt aacggaggcg cgggcggtaa taggctgatt ttcaggtatt accggcttac
       61 agcgtgtggt tgatattgag gggctgtgag tttcgcgtat ttgtcagaaa cccagacgag
      121 ttttcctgta tgggacgaaa taaggcagcg gcaatacctg gaaaaattta atatggtatg
      181 aaggagaacc tgtcggttac gcttaatata cccggtacac tgtcagctca ggggatacat
      241 gaaccgttat ctcaccagct
//
LOCUS       DAPOZO010000349          259 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPOZO010000349 DAPOZO010000000
VERSION     DAPOZO010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.357"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..243
                     /locus_tag="ROI21_004978"
     CDS             <1..243
                     /locus_tag="ROI21_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HDX5113761.1"
                     /translation="EGWVLAVISEIQSFALTDDERELMQQWLEHKYPQTQTTRARKPG
                     RSRNGDASRYAGFREGQNVRLHRPVSGREQQKLEAR"
BASE COUNT           71 a           63 c           83 g           42 t
ORIGIN      
        1 gaaggctggg tgctggccgt tatcagtgaa atacagtcct tcgccttgac cgatgacgaa
       61 cgtgaactga tgcagcagtg gctggaacat aaatacccgc agacgcaaac caccagggcg
      121 cgtaaaccgg gaagaagtcg caatggcgac gcctcgcgct atgcggggtt tcgagaaggg
      181 cagaacgtca gactgcaccg accggtcagt gggcgggaac agcagaaact ggaggccaga
      241 tgattacgtt atcaggtaa
//
LOCUS       DAPOZO010000350          259 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPOZO010000350 DAPOZO010000000
VERSION     DAPOZO010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.358"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..243
                     /locus_tag="ROI21_004979"
     CDS             <1..243
                     /locus_tag="ROI21_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HDX5113762.1"
                     /translation="EGWVLAVISEIQSFALTDDERELMQQWLEHKYPQTQTTRARKPG
                     RSRNGDASRYAGFREGQNVRLHRPVSGQEQQKLEAR"
BASE COUNT           70 a           65 c           82 g           42 t
ORIGIN      
        1 gaaggctggg tgctggccgt tatcagtgaa atacagtcct ttgccctgac cgatgatgag
       61 cgtgaactga tgcagcagtg gctggaacat aaatacccgc agacgcaaac caccagggcg
      121 cgtaaaccgg gaagaagccg caatggcgac gcctcgcgct atgcggggtt tcgtgaaggg
      181 cagaacgtca gactgcaccg cccggtcagt gggcaggaac aacagaaact ggaggccaga
      241 tgattacgct atcaggtaa
//
LOCUS       DAPOZO010000351          258 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPOZO010000351 DAPOZO010000000
VERSION     DAPOZO010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.359"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>258
                     /locus_tag="ROI21_004980"
     CDS             <1..>258
                     /locus_tag="ROI21_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113763.1"
                     /translation="QLDKLRRMNVGSCSEKVSRRIAQMEADLKALQKESDTLTGRVDD
                     PAVQRPLRQTRTRKPFPESLPRDEKRLLPAASCCPECGGSLS"
BASE COUNT           58 a           85 c           74 g           41 t
ORIGIN      
        1 cagctggata agctgcgccg gatgaacgtc ggcagctgct ccgaaaaagt ctcccgtcgt
       61 atcgcacaga tggaagctga cctgaaggca cttcagaaag aaagtgatac ccttaccggt
      121 cgggttgacg acccggccgt gcagcgcccg ctgcgtcaaa cccgcacccg caaaccgttc
      181 cccgaatcac tcccccgcga tgaaaaacgg ctgctgccgg cagcgtcatg ctgcccggaa
      241 tgtggaggct cgctgagc
//
LOCUS       DAPOZO010000352          255 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.360, whole genome shotgun sequence.
ACCESSION   DAPOZO010000352 DAPOZO010000000
VERSION     DAPOZO010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.360"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT           35 a           90 c           78 g           52 t
ORIGIN      
        1 ggtttcgggc tgtcgctacg gtccgatgcg tgaaaacccc tggcttgcag cgaccgttcc
       61 ggtcgcgccg cctctgtcgc gccgtggcct gcgctcctgg catcccggca aaagaccgcc
      121 gggatgtaag gggcaacgcc gtttcgcaaa ccgcgcttca cgtcgttgcg gcgggtccct
      181 gcccgttgtt ctgtccggtg ccattcacct gtttaagccc ctgtcgccgg gggcaaagac
      241 agccacaacc gtctc
//
LOCUS       DAPOZO010000353          255 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPOZO010000353 DAPOZO010000000
VERSION     DAPOZO010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.361"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            3..>255
                     /locus_tag="ROI21_004981"
     CDS             3..>255
                     /locus_tag="ROI21_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113764.1"
                     /translation="MEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEPPKYTPRP
                     AVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYR"
BASE COUNT           72 a           65 c           62 g           56 t
ORIGIN      
        1 ttatggaaat taaaatcctg cacaagcagg gaatgagtag ccgggcgatt gccagagaac
       61 tggggatctc ccgcaatacc gttaaacgtt atttgcaggc aaaatctgag ccgccaaaat
      121 atacgccgcg acctgctgtt gcttcactcc tggatgaata ccgggattat attcgtcaac
      181 gcatcgccga tgctcatcct tacaaaatcc cggcaacggt aatcgctcgc gagatcagag
      241 accagggata tcgtg
//
LOCUS       DAPOZO010000354          254 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPOZO010000354 DAPOZO010000000
VERSION     DAPOZO010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.362"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>254
                     /locus_tag="ROI21_004982"
     CDS             <1..>254
                     /locus_tag="ROI21_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113765.1"
                     /translation="ARSTLYYQLSLQKAKDKYADVKQLIASIFHEHRGCYGYRRIHCE
                     LQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLA"
BASE COUNT           71 a           61 c           69 g           53 t
ORIGIN      
        1 gccagaagca cgctgtacta ccagctgtca ctgcagaagg ctaaggacaa gtatgctgat
       61 gtgaaacagc tcatagcctc catcttccat gaacaccggg gatgttatgg gtaccggcgg
      121 atccattgtg agcttcagaa aagaggtctt aagttcagtg gcaaaacagt gcgaaaactg
      181 atgcaacagc tcggcctgaa gtctcctgtg cgcctgaaga aataccggtc ttaccgggga
      241 aatatgggac tcgc
//
LOCUS       DAPOZO010000355          253 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.363, whole genome shotgun sequence.
ACCESSION   DAPOZO010000355 DAPOZO010000000
VERSION     DAPOZO010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.363"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT           72 a           57 c           59 g           65 t
ORIGIN      
        1 tatatgtgaa ataaatctgt gagtgtgatc agcagaccag tctacccagt agcaacagat
       61 tgcgttgtac tcacagggtt atccctgata actggcgtat aacctgcgta cataaaacgt
      121 acctgcaatg cacctgaaca gagtgcatta agcatccgcc tgagcggtaa acgtatccga
      181 tctggtgagt gacagtctga gagcattctc agtaatcgcc cccttgtatc ctggaaattg
      241 gttaagagcg att
//
LOCUS       DAPOZO010000356          248 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPOZO010000356 DAPOZO010000000
VERSION     DAPOZO010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.364"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT           60 a           71 c           61 g           56 t
ORIGIN      
        1 tgactggttc agatgaaagg agaccaccgc aaacgcgtcc ggtggcaggc cgtccacttc
       61 cagcgtgaaa cgtaatccgg ttgacatagc aacctccggg tttaaggaaa aacagacagt
      121 tcgctgttaa agcggctgta acagtacagt gttacgcttg cgttcaggca cgctaccgcc
      181 gaagtttccg gcatccgcca tctcccgtga acgtaacccc ttactgacaa cctttctttt
      241 tacggcgt
//
LOCUS       DAPOZO010000357          246 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPOZO010000357 DAPOZO010000000
VERSION     DAPOZO010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.365"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..244)
                     /locus_tag="ROI21_004983"
     CDS             complement(<1..244)
                     /locus_tag="ROI21_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HDX5113766.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSI"
BASE COUNT           92 a           38 c           31 g           85 t
ORIGIN      
        1 taattgaggt attaaattgt tttgctgttt cattaaatga ctttaaataa tcatcatcca
       61 cataatcagt gaaaacatga aagacaaaat taatatctga attattaatc aaaacggaag
      121 aaattgaaac gccagcacca tacaaaaaat tcttatcgat accgtaggaa acatttagct
      181 ctggggcgtt ttctttttta ttatctgcta aatacgaaaa ccgttctttt ataaattcat
      241 tcattt
//
LOCUS       DAPOZO010000358          244 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPOZO010000358 DAPOZO010000000
VERSION     DAPOZO010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.366"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT           83 a           51 c           39 g           71 t
ORIGIN      
        1 gaaatgtttt catatttagt gtactgtata cggccattta tacaggaaaa gcctatgtca
       61 gaacgtaaaa actcaaaatc acgccgtaat tatctcgtta aatgttcctg cccaaactgc
      121 acccaagagt cagaacacag tttttcaaga gtacaaaaag gtgccctttt gatctgccct
      181 cattgcaaca aagtattcca gacaaatctt aaagctgtag cctgattgat tttattagta
      241 acaa
//
LOCUS       DAPOZO010000359          243 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPOZO010000359 DAPOZO010000000
VERSION     DAPOZO010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.367"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>243
                     /locus_tag="ROI21_004984"
     CDS             <1..>243
                     /locus_tag="ROI21_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDX5113767.1"
                     /translation="LEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDD
                     AGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASSDNN"
BASE COUNT           53 a           66 c           77 g           47 t
ORIGIN      
        1 ctggagggtg acctgctcag aacagaggtc gccggtatgt cactgacgac aggagtgtat
       61 ggtgctgcag gccattcttc cgttgatgtt aaggatgatg acggctcccg cgccggcacg
      121 gtccgggatg atgccggcag cttgggcgga tatatgaacc tgacacacac ctcctccggc
      181 ctgtgggctg acattgtggc acagggaacc cgccacagca tgaaagcgtc atcggacaat
      241 aac
//
LOCUS       DAPOZO010000360          241 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPOZO010000360 DAPOZO010000000
VERSION     DAPOZO010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.368"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>241
                     /locus_tag="ROI21_004985"
     CDS             <1..>241
                     /locus_tag="ROI21_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113768.1"
                     /translation="TANGLILMKLPETWSTNEKMFASGGQGHGFAAERGNHIVDRVRL
                     KNARILGDNNARNGADRLVSGTEIQTKYCSTAARSV"
BASE COUNT           74 a           52 c           68 g           47 t
ORIGIN      
        1 caacggcgaa tggtctgatt ctcatgaaac tgccggaaac ctggagtaca aatgagaaaa
       61 tgtttgcttc cggagggcag ggacatgggt ttgccgctga acggggaaac catattgtcg
      121 acagagtccg tctgaaaaac gcacggatcc tcggtgataa taatgccaga aatggagcag
      181 acagactggt cagcggaaca gaaatccaga cgaaatattg ttcaactgca gcccgtagcg
      241 t
//
LOCUS       DAPOZO010000361          241 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPOZO010000361 DAPOZO010000000
VERSION     DAPOZO010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.369"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>241
                     /locus_tag="ROI21_004986"
     CDS             <1..>241
                     /locus_tag="ROI21_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113769.1"
                     /translation="LLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGW
                     AEADNNIAENALRMVSLGRKNYLFFGSDHGGERGAL"
BASE COUNT           59 a           58 c           78 g           46 t
ORIGIN      
        1 gctgctgaaa tccctggaaa gctggctgcg tgaaaagatg aaaaccctgt cgcgacactc
       61 agaactggcg aaagcgttcg catacgccct gaaccagtgg ccggcgctga cgtactatgc
      121 agatgatggc tgggctgagg cggacaataa catcgctgaa aatgcgttgc ggatggtcag
      181 tctgggccgc aaaaactacc tgttcttcgg ttcggatcat ggaggagagc ggggagcgct
      241 g
//
LOCUS       DAPOZO010000362          239 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPOZO010000362 DAPOZO010000000
VERSION     DAPOZO010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.370"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>239
                     /locus_tag="ROI21_004987"
     CDS             <1..>239
                     /locus_tag="ROI21_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113770.1"
                     /translation="NGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDP
                     AEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEG"
BASE COUNT           64 a           61 c           64 g           50 t
ORIGIN      
        1 aacggacagt atcgttacat gggaaataat ggccccatgc aactggaagt cccccgtgat
       61 cagtatgccg gcgctgttga aaccatgagg aataagatcc gcgaaggtaa agtacccggt
      121 gtaaccgatc ccgcagaagc gtcacggctg attcgtcggg gacatctgac ttatactcag
      181 gcccgtaata tcacccggtt cgggaccatc gaatcagtca cttatgatat tgccgaggg
//
LOCUS       DAPOZO010000363          239 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPOZO010000363 DAPOZO010000000
VERSION     DAPOZO010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.371"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>239
                     /locus_tag="ROI21_004988"
     CDS             <1..>239
                     /locus_tag="ROI21_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113771.1"
                     /translation="NGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVPGVTDP
                     EEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEG"
BASE COUNT           63 a           62 c           65 g           49 t
ORIGIN      
        1 aacggacagt atcgttacat gggaaatcat ggtcccatgc aactggaagt cccccgtgat
       61 cagtatgccg gcgctgtgga aaccatgaag aataagatcc gcgaaggtaa agtacccggt
      121 gtaaccgatc ccgaagaagc gtcccggctg attcgtcggg gacatctgac ttatacccag
      181 gcccgtaata tcacccggtt cgggaccatc gaatcggtca cttatgatat tgccgaggg
//
LOCUS       DAPOZO010000364          236 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.372, whole genome shotgun sequence.
ACCESSION   DAPOZO010000364 DAPOZO010000000
VERSION     DAPOZO010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.372"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT           72 a           55 c           51 g           58 t
ORIGIN      
        1 tatgccgccc ttacgatgta attaaacgca atgttacggg gacgaatggt cacataatta
       61 accccatcgc ctcgcgaagg acctgcaaat gaaaaacggg agaatccagg ttggttgtca
      121 acgatttggt catagttatt gattgaatga ccagacccaa tgtcaaaatt acctgcgtaa
      181 tgagaaaaac ttgtaccatc ctgccgtgaa agcagatcgc gccctgtatc cacttt
//
LOCUS       DAPOZO010000365          233 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPOZO010000365 DAPOZO010000000
VERSION     DAPOZO010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.373"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>233
                     /locus_tag="ROI21_004989"
     CDS             <1..>233
                     /locus_tag="ROI21_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDX5113772.1"
                     /translation="LKDSSQAGFSHVLVIEEAHSLPIPTLKHLKRFFELESGFKKLLS
                     IVLIGQPELATKLSERNMEVREVVQRCEVVELLP"
BASE COUNT           55 a           61 c           65 g           52 t
ORIGIN      
        1 ctgaaagaca gcagccaggc ggggttcagc cacgttctgg tgattgagga ggcccacagt
       61 ctgcccattc cgacactgaa acacctcaaa cgcttttttg agctggagtc cggtttcaaa
      121 aaactgctgt ccatcgtgct gattggccag ccggaactgg cgacaaaact gtctgaacgc
      181 aatatggaag tccgtgaagt cgttcagcgc tgtgaggtgg tcgaacttct gcc
//
LOCUS       DAPOZO010000366          228 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.374, whole genome shotgun sequence.
ACCESSION   DAPOZO010000366 DAPOZO010000000
VERSION     DAPOZO010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.374"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>228
                     /locus_tag="ROI21_004990"
     rRNA            <1..>228
                     /locus_tag="ROI21_004990"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           65 a           49 c           75 g           39 t
ORIGIN      
        1 gtgaagaggg aaacaaccca gaccgccagc taaggtccca aagtcatggt taagtgggaa
       61 acgatgtggg aaggcccaga cagccaggat gttggcttag aagcagccat catttaaaga
      121 aagcgtaata gctcactggt cgagtcggcc tgcgcggaag atgtaacggg gctaaaccat
      181 gcaccgaagc tgcggcagcg acactatgtg ttgttgggta ggggagcg
//
LOCUS       DAPOZO010000367          220 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.375, whole genome shotgun sequence.
ACCESSION   DAPOZO010000367 DAPOZO010000000
VERSION     DAPOZO010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.375"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            15..>220
                     /locus_tag="ROI21_004991"
     CDS             15..>220
                     /locus_tag="ROI21_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113773.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNV"
BASE COUNT           69 a           55 c           48 g           48 t
ORIGIN      
        1 ggtggacgct atccatgaaa tcattaaccg cagtgcgtaa aaaaagccct aattatcccg
       61 ttgagttcaa aatcaaaatg gttgaactct cgcatcgacc agagatctcc gtagcgcaac
      121 tcgctcgtga gcatgggatc aacgataatt tgctgttcaa gtggcgccag tactggcgcg
      181 aaggaaaact acgtcctcct tcaacaacag aaaacaacgt
//
LOCUS       DAPOZO010000368          213 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.376, whole genome shotgun sequence.
ACCESSION   DAPOZO010000368 DAPOZO010000000
VERSION     DAPOZO010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.376"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            62..>213
                     /locus_tag="ROI21_004992"
     CDS             62..>213
                     /locus_tag="ROI21_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113774.1"
                     /translation="MILWSMKKETDIRRGKHCVFLMHVHLVFVTRYRRQIFDHDAKEK
                     LRTYFS"
BASE COUNT           61 a           47 c           41 g           64 t
ORIGIN      
        1 ctcctgttga ccacttggtc ttaactggaa tttaaatgcc tgtaatcgtt tcacaggtca
       61 aatgatactt tggtctatga aaaaagaaac cgatatccgg cgtggaaagc attgtgtttt
      121 cctgatgcat gttcacctgg tctttgtcac cagataccga cgccagattt ttgaccatga
      181 cgcgaaagaa aaactacgca cttacttttc aaa
//
LOCUS       DAPOZO010000369          212 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.377, whole genome shotgun sequence.
ACCESSION   DAPOZO010000369 DAPOZO010000000
VERSION     DAPOZO010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.377"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
BASE COUNT           55 a           50 c           44 g           63 t
ORIGIN      
        1 ctcctgttga ccaccgggtc ttaactggaa tttaaatgcc tgtcgtcgtt tcacaggtca
       61 aattatattt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
      121 cctgatgcat gttcacctgg tctttgtccc aaataccgac gccagatttt tgaccatgac
      181 gcgacagaaa aaccacgcac ttacttttca ga
//
LOCUS       DAPOZO010000370          212 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.378, whole genome shotgun sequence.
ACCESSION   DAPOZO010000370 DAPOZO010000000
VERSION     DAPOZO010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.378"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..>212
                     /locus_tag="ROI21_004993"
     CDS             <1..>212
                     /locus_tag="ROI21_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDX5113775.1"
                     /translation="LREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFP
                     QPLHDALDKFQADTGIDIDMHIDAASG"
BASE COUNT           48 a           67 c           57 g           40 t
ORIGIN      
        1 ctgcgtgaga tccctatgcg ccccggtcag ttgtttatgg acccgaaacg catgattgaa
       61 gcctgcgacg aaaacaccat cggcgtggtg ccgactttcg gcgtgaccta caccggtaac
      121 tatgagttcc cgcaaccgct gcacgatgcg ctggataaat tccaggccga caccggtatc
      181 gacatcgaca tgcacatcga cgccgccagc gg
//
LOCUS       DAPOZO010000371          210 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.denovo.379, whole genome shotgun sequence.
ACCESSION   DAPOZO010000371 DAPOZO010000000
VERSION     DAPOZO010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.denovo.379"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..125)
                     /locus_tag="ROI21_004994"
     CDS             complement(<1..125)
                     /locus_tag="ROI21_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDX5113776.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSIL"
BASE COUNT           48 a           50 c           40 g           72 t
ORIGIN      
        1 ctcaaaatac tgcctattgc tctacgcttc tctctgtaga acacctgtct tcggtatttt
       61 ggcgcaaaaa ctatgtgata tttacagttc catcgggtgt gcgctaagct cttttcgttc
      121 cccattggga cccccttttg atttcttgtt tgacacttgc agttgccaga ccgcaaggtg
      181 ttttaacaaa tcaaaagggg ttttaataac
//
LOCUS       DAPOZO010000372         8316 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.guided.156, whole genome shotgun sequence.
ACCESSION   DAPOZO010000372 DAPOZO010000000
VERSION     DAPOZO010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.guided.156"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(<1..312)
                     /locus_tag="ROI21_004995"
     CDS             complement(<1..312)
                     /locus_tag="ROI21_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDX5113777.1"
                     /translation="MSQASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEQKRLTLRVPHHIDSRLTIVARQHSISKN
                     QL"
     gene            complement(391..2103)
                     /locus_tag="ROI21_004996"
                     /pseudo
     CDS             complement(391..2103)
                     /locus_tag="ROI21_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            2075..2860
                     /gene="lafU"
                     /locus_tag="ROI21_004997"
     CDS             2075..2860
                     /gene="lafU"
                     /locus_tag="ROI21_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDX5113778.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            2931..3986
                     /gene="dinB"
                     /locus_tag="ROI21_004998"
     CDS             2931..3986
                     /gene="dinB"
                     /locus_tag="ROI21_004998"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDX5113779.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            4038..4331
                     /gene="yafN"
                     /locus_tag="ROI21_004999"
     CDS             4038..4331
                     /gene="yafN"
                     /locus_tag="ROI21_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HDX5113780.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            4334..4732
                     /gene="yafO"
                     /locus_tag="ROI21_005000"
     CDS             4334..4732
                     /gene="yafO"
                     /locus_tag="ROI21_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HDX5113781.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            4742..5194
                     /locus_tag="ROI21_005001"
     CDS             4742..5194
                     /locus_tag="ROI21_005001"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDX5113782.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFSMRYKPQH"
     gene            5439..5645
                     /locus_tag="ROI21_005002"
                     /pseudo
     CDS             5439..5645
                     /locus_tag="ROI21_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            5641..5993
                     /gene="prfH"
                     /locus_tag="ROI21_005003"
                     /pseudo
     CDS             5641..5993
                     /gene="prfH"
                     /locus_tag="ROI21_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(6050..7507)
                     /gene="pepD"
                     /locus_tag="ROI21_005004"
     CDS             complement(6050..7507)
                     /gene="pepD"
                     /locus_tag="ROI21_005004"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDX5113783.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            7768..8226
                     /gene="gpt"
                     /locus_tag="ROI21_005005"
     CDS             7768..8226
                     /gene="gpt"
                     /locus_tag="ROI21_005005"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDX5113784.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
BASE COUNT         2035 a         2115 c         2126 g         2040 t
ORIGIN      
        1 gagctggttt tttgaaatgg aatgttgtct ggcgacgatg gtcagccgcg aatcaatgtg
       61 atgaggaaca cgaagcgtca ggcgtttttg ctcttcttca cgaaaagggg caatgccgtc
      121 ctcttcacaa tccgccatcc actcatccag cgcactttca ccttcccggt ataacgcctc
      181 aatgcttgtc gctacaaagt cgcaataacc atttacattg agaaatttgc cgcgaaatgc
      241 gttgatatcg gcttcaaatg tcactgcggc tggttgacct ttaatggtga taatgttcga
      301 tgcttgagat attaataaac gcgttttcat catcttatca atccctgacg tcataaattg
      361 atgtcatcgt attgtgtttc ttaaaaggca tcaactcagc gcccccataa tcttcaactc
      421 cagctcatcc ggcacttcgt tatacgacag cacatgcagc cccggcgcaa acaaccttgc
      481 atagcgcgcc agcaatggac gcagttgcgg cggcaccagc agcaccgggt ctttccccgc
      541 cgctttcatc tgctctttta cctgcggcat cgtgctctgg aactggttaa gcatattcgg
      601 gtccaccggc acactgtcga gcatcacttt gccggcctgt tgcgcttgat tcactacgtt
      661 ggtcagcaga ttttccagct cattattcag cgtatacacc gtcagctcct gcttgcgaac
      721 gaacggatgg gtaatgctgc gccgcagcgc caggcgcaca tctgccgcca gcagaatatg
      781 atctttggtc accgcgctac tggcaaccag cacggtggcg atggtgacga tatcgcgcag
      841 ggaaacgcct tcggtcagca gcgcccggta cactttcagc aactggctgt aattgagcgc
      901 cgcgcttaaa tcctccgcca gtcgcggtgc catcgacgac aaacggttat gcaactgcgt
      961 gatgtcatca tagttaaata gatcaggaat atagctgcgc acaatcttgt tcacatgcgt
     1021 ggcaatcacg ctggcgctgt cgatcacctg ataccccata ttcagcgctt tcgctttctg
     1081 cgcgggctgg atccaggtca ccggcatccc gtacgccggg tcgttcccca gcacgccgtc
     1141 tatctcgccg taggtttcgc tggagggcag cgccatcagt ttatccgccg gaatatccgc
     1201 ttcatcggcc ttaatgccgt tgatgaaaat ggcgtactga ctgggcttga ggcggaagtt
     1261 ttcccgaata cggatctccg gcagcagcac gccgttgccg tcggatatca cttgccgcac
     1321 gccgcgaata cgctgggtga gtgggttacc ctgcgcttta tcgaccagcg caaccagctt
     1381 gtagccgagg cttaagctga taggttcgat cagcggaata gtttcccaac tgacctgttg
     1441 ctcgctggtt tcggtgatgg tgcgggtcag cgtttcgagg cttttctctt ccgcctccgc
     1501 cgcctgcggg cgtttgctca tccgccagcc ggtaaagcca agcagggcgc tgaacagcaa
     1561 aaatggcaga tgcggcatcc ccggcaccac cgccagcaca aacatgatcc cggtagcggt
     1621 gtagagcacc gacgggcttg ccagcaactg gttacgcacg tcatgggcaa tatcgccgtt
     1681 gtcgctgacg cgggtgacga taatcgccgc cgcggtggaa agcagcaggg acgggatctg
     1741 cgccaccagg ccgtcgccga tggtcatcag cacatattgc tggaaggcgg catcggcgct
     1801 caggttgtat ttgaagatcc cgatacagac gccgccgatc aggttgatcg ccagaatcat
     1861 catcccggcg atggcatccc cgcgcacaaa cttcgatgcc ccgtccattg cgccgtagaa
     1921 atcggcctcg ctggcaacat ctttacgccg ggtttgcgcc tgcgcctggt tgatcaaccc
     1981 ggcgttaaga tcggcgtcaa tcgccatctg tttgccgggc atcgcgtcca gggtaaagcg
     2041 ggcagaaacc tcggaaatac gctcggcccc tttggtgacg acgataaagt tgattgtcaa
     2101 cagcgtcagt aaatccgagc gtgaaagcat tatcgccgcg ctgcacgggc agtcgatttt
     2161 caatggcggc gggttgtcgc cgttaaataa aatcagcccg tctcatccgc caaaacctgc
     2221 caccgtggcg gtgccggaag agacggagaa aaaagcgcgt gacgtcaatg agaaaacggc
     2281 gctgctgaag aagaaatccg ctactgagct tggcgagctg gcgaccagta tcaacaccat
     2341 tgcccgtgat gcacatatgg aagcgaatct ggagatggag attgttcccc agggattgcg
     2401 cgtgctgatt aaagatgacc agaaccgcaa tatgtttgaa cgcggcagcg cacagattat
     2461 gccgttcttt aaaactttgc tggtggagct cgcgccagtg ttcgactcgc tcgataacaa
     2521 aattattatt acagggcata ccgatgcgat ggcctacaaa aacaatatct acaacaactg
     2581 gaacctttcg ggcgatcgcg cgctttcggc ccgtcgggtg ctggaagagg ctggaatgcc
     2641 ggaagataaa gtgatgcagg taagcgcaat ggcggaccag atgctgctgg atgccaaaaa
     2701 tccgcaaagc gcgggcaacc gacgcattga gattatggtg ctgaccaaaa gtgcgtccga
     2761 tacgctgtat caatactttg gtcagcatgg ggataaagtg gtgcagccgc tggtgcaaaa
     2821 gctggataag cagcaggtgc tttcgcagcg aacgcgctag atgctgaatc tttacgcatt
     2881 tctcaaaccc tgaaatcact gtatacttta ccagtgttga gaggtgagca atgcgtaaaa
     2941 tcattcatgt ggatatggac tgctttttcg ccgcagtgga gatgcgcgac aatcccgccc
     3001 tgcgcgatat ccctattgct attggcggca gccgcgaacg tcggggggtg atcagcaccg
     3061 ccaattatcc cgcgcgtaaa tttggcgtac gtagcgctat gccgacaggg atggcgctca
     3121 aattatgccc acatctcacc ttgcttccgg ggcgctttga cgcctacaaa gaagcctcaa
     3181 atcatatccg tgaaatcttc tcgcgctaca cctcgcgtat tgaaccgttg tcactggatg
     3241 aagcctatct cgatgtcact gatagcgtcc attgccacgg ttctgcgacc ctcatcgccc
     3301 aggaaatccg ccagacaatc ttcaacgagc tgcaactgac ggcgtctgcg ggcgtggcac
     3361 cagtaaagtt tctcgccaaa atcgcctccg acatgaataa acccaacggc cagtttgtga
     3421 ttacgccggc agaagttccg acatttttac aaaccttacc gctggcaaaa atccccggcg
     3481 tcggcaaagt ctcggcggca aaactggaag cgatggggct gcggacctgc ggtgatgtgc
     3541 aaaagtgtga tctggtgact ctgctcaaac gttttggcaa atttggccgc attttgtggg
     3601 agcgtagtca ggggattgac gagcgcgacg ttaacagcga acggttgcga aaatctgtcg
     3661 gcgtggaacg cacgatggcg gaagatattc atcactggtc tgaatgtgaa gcgattatcg
     3721 agcggctgta tccggaactt gaacgtcgtc tggcaaaagt aaaacctgat ttactgattg
     3781 ctcgccaggg ggtgaaatta aagttcgacg attttcagca aactactcag gagcacgtct
     3841 ggccgcggct gaataaagct gatctaatcg ccaccgcgcg taaaacctgg gatgaacgcc
     3901 gcggcgggcg cggtgtgcga ctggtggggc tgcatgtgac gttgcttgac cctcaaatgg
     3961 aaagacaact ggtgctggga ttatgatgta tactattatg tctattctgg tgtgcattat
     4021 tatgagggga tcactgtatg catcgaattc tcgctgaaaa atcggtcaat atcactgagt
     4081 tacgtaaaaa cccagctaaa tactttattg atcaaccggt tgcggttctt tctaataatc
     4141 gccccgcagg atatctctta agtgccagcg cattcgaagc gttaatggac atgcttgctg
     4201 aacaagagga gaaaaagccc ataaaggcgc gcttccgtcc gagtgctgca agattagagg
     4261 aaattacacg ccgcgctgaa caatatctta atgatatgac ggatgatgat ttcaatgact
     4321 ttaaggaata aggatgcggg tattcaaaac aaaacttatt cgcctgcaac ttacagcaga
     4381 ggaacttgat gcgttaacgg cggattttat ttcctataag cgtgacggta ttttgccaga
     4441 tatatttggt cgcgatgcac tctacgacga ctcgtttacc tggccattaa tcaaatttga
     4501 gcgagttgct catattcatc tggcaaatgt gaataatcca tttccgccac agttgcgcca
     4561 attcagcaga acgaatgacg aagcgcattt ggtatattgt cagggggcgt ttgatgagca
     4621 agcatggttg ctcattgcca ttctgaagcc tgaacctcat aaactggctc gagataacaa
     4681 ccaaatgcat aaaattggaa aaatggcaga agcgtttcgc atgcgttttt gaatttatat
     4741 tatgaataac atacaaataa gagattatca gcctggcgat tttcagcaac tatgcgctat
     4801 tttcattaga gcggttacga tgaccgccag tcagcattat tcaccacaac aaattgccgc
     4861 ctgggcgcag attgacgaat ctcgctggaa ggagaaactc gcgaaatcac aagtgcgggt
     4921 tgcagtcatt aatgcaaaac tggtcggctt tattacctgc gttgaacact atatcgatat
     4981 gttatttgtt gaccctgaat acacccgccg tggggttgcc agcgctttgt taaaaccttt
     5041 gattaagtct gaatccgaac ttacagtgga cgccagcata accgcaaaac ccttttttga
     5101 acgttatggt tttcagacag ttaagcagca gcgcgttgaa tgcaggggag cgtggtttac
     5161 taatttttct atgcgatata aaccgcaaca ttaaatccag cttgcaatga aaataacgcc
     5221 cgcctggtat gtgcgcgttt tcttattcca caaactgcaa ggaggtaaat catgacaaat
     5281 cctttatcaa tgactctttg cagacctttc caggattaat tcttttttct tgccctggat
     5341 tcgtctgcca tttcctgatt tttatattta tatactctaa ataattcgag tcgcagtaca
     5401 tgcaacttga agtatgacga gtataaatgg aatgatacat gggcaaatat attcgtccct
     5461 tatccgatgc ggtatttacc atcgcatctg atgacctgtg gatcgagagt ttagcgattc
     5521 aacaattaca caccacggca aatttaccca acatgcagcg cgtagtaggg atgccagatt
     5581 tacaccccgg acgcggctac ccgattggcg cagcgttatt ctctgttggt cgtttttacc
     5641 cggcaagacg tcgcggtaac ggtgctggaa acagaaacgg gccgctactc tgacacgctg
     5701 cgttcggcgc tgatttctct ggatggcgat aacgcatggg cattaagcga aagttggtgc
     5761 ggcactattc agtggatttg tccgagtccg tatcggcctc atcatgggcg caaaaacttg
     5821 tttctgggca tgctaacaag cggctggcgc gattgctgat tgcctggacg ctggaacaac
     5881 agcaacagga aaatagcgcg gtgctgaaat cgcggcggcg aatgttccat caccagattg
     5941 aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga taatgaagga
     6001 tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat tacttcgccg
     6061 gaatttcttt cagcagctca gtcagcagtg tccagtaatg acctacgctt tcgatgtgaa
     6121 cttgctcatc cggagagtgt ggaccggtga ttgttggccc gatagaaacc atgtccattt
     6181 ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc tggatgttcg
     6241 gcgtcttgtt gaacagacgc tgataagttt cacgtaccag atgcatcacc ggagaattag
     6301 cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca gccagtttac
     6361 ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga gaacggatca
     6421 ggcagtgaat ttctacgtta ttgtcagtca tggtcaccac accgacgttc agggaggttt
     6481 ccaccacacc ttttgccaca tcggaattgc ggatcacacc gttcggggtg gcgttcagca
     6541 gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc gcaacagagt
     6601 ccagcagcaa tgccagattt ttctctttct ctgccagctc gtttttcagg atctcctgat
     6661 aggtattcac cagagatttc agggcgtcga ctttatcagc tgcgacagca atggtcgcaa
     6721 aggcttcacg cgggatggcg ttacgcagtg tgccaccgtt gaaatcgata aggcgcaggt
     6781 ccagctcttc tgcatgaccc gccaggaagc gcaccagcag tttgttggca ttacccaggc
     6841 caacgtggat ttcaccgccg gagtgaccgc ctttcaggcc ttttaaggtt aacttgaagg
     6901 tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg aagtcgatac
     6961 cccccgcgca acccatgtag atttcacctt cctcttcgga gtcggtgtta atcagaatat
     7021 cagcctgcaa ccagttgctc tgtaagccaa aagcaccatc cataccggct tcttcggtca
     7081 tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga acagccagcg
     7141 cagacgccat accaatgccg ttatccgcac ccagcgtggt gccgcgcgct ttaacccatt
     7201 cgccatcaat ataaggctgg ataggatctt tagtgaagtc atgcacggtg tcgttatttt
     7261 tctgcggcac catatcgagg tgggcctgca agacgactgg tttacgattt tccatacctg
     7321 cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca tggaaacctt
     7381 tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag gacgggtgag
     7441 gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat tgagacagtt
     7501 cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt cacagggtta
     7561 gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat accactttat
     7621 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
     7681 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
     7741 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
     7801 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
     7861 attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg tgaactgggt
     7921 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     7981 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
     8041 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
     8101 atcttcgcaa aaccggctgg tcgcccgctg gttgatgatt atgttgttga tatcccgcaa
     8161 gatacctgga tcgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
     8221 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
     8281 acaatagttt ccagtaagta ttctggaggc tgcatc
//
LOCUS       DAPOZO010000373         3891 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.guided.200, whole genome shotgun sequence.
ACCESSION   DAPOZO010000373 DAPOZO010000000
VERSION     DAPOZO010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.guided.200"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            complement(39..473)
                     /gene="intI1"
                     /locus_tag="ROI21_005006"
                     /pseudo
     CDS             complement(39..473)
                     /gene="intI1"
                     /locus_tag="ROI21_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
     gene            631..1104
                     /locus_tag="ROI21_005007"
     CDS             631..1104
                     /locus_tag="ROI21_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HDX5113785.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            1235..2023
                     /locus_tag="ROI21_005008"
     CDS             1235..2023
                     /locus_tag="ROI21_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HDX5113786.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            2229..2576
                     /locus_tag="ROI21_005009"
     CDS             2229..2576
                     /locus_tag="ROI21_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HDX5113787.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            2570..3409
                     /gene="sul1"
                     /locus_tag="ROI21_005010"
     CDS             2570..3409
                     /gene="sul1"
                     /locus_tag="ROI21_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HDX5113788.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            3537..3740
                     /locus_tag="ROI21_005011"
     CDS             3537..3740
                     /locus_tag="ROI21_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDX5113789.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
BASE COUNT          873 a         1039 c         1064 g          915 t
ORIGIN      
        1 tgataagttt atcaccaccg actatttgca acagtgccca tgcccgttcc atacagaagc
       61 tgggcgaaca aacgatgctc gccttccaga aaaccgagga tgcgaaccac ttcatccggg
      121 gtcagcacca ccggcaagcg ccgcgacggc cgaggtcttc cgatctcctg aagccagggc
      181 agatccgtgc acagcacctt gccgtagaag aacagcaagg ccgccaatgc ctgacgatgc
      241 gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg
      301 ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg
      361 acataagcct gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg
      421 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
      481 tatgactgtt tttttgtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
      541 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
      601 aaaacaaagt tagccattaa gggagttaaa ttgaaaatat cattgatttc tgcagtgtca
      661 gaaaatggcg taatcggtag tggtcctgat atcccgtggt cagtaaaagg tgagcaacta
      721 ctctttaaag cgctcacata taatcaatgg ctccttgtcg gaagaaaaac atttgactct
      781 atgggtgttc ttccaaatcg caaatatgca gtagtgtcaa agaacggaat ttcaagctca
      841 aatgaaaacg tcctagtttt tccttcaata gaaaatgctt tgaaagagct atcaaaagtt
      901 acagatcatg tatatgtctc tggcgggggt caaatctata atagccttat tgaaaaagca
      961 gatataattc atttgtctac tgttcacgtt gaagtcgaag gtgatatcaa attccctata
     1021 atgcctgaga atttcaattt ggtttttgaa cagtttttta tgtctaatat aaattataca
     1081 taccagattt ggaaaaaagg ctaacaatgc gttgcagcac cagtcgcttc gctccttgga
     1141 cagcttttaa gtcgcgtctt tgtggttttg ctgcgcaaaa gtattccaca aagccgcaac
     1201 ttaaaagctg ccgctgaact taacgttagg catcatgggt gaatttttcc ctgcacaagt
     1261 tttcaagcag ctgtcccacg ctcgcgcggt gatcgagcgc catctggctg cgacactgga
     1321 cacaatccac ctgttcggat ctgcgatcga tggagggctg aagccggaca gcgacataga
     1381 cttgctcgtg accgtcagcg ccgcacctaa cgattcgctc cggcaggcgc taatgctcga
     1441 tttgctgaaa gtctcatcac cgccaggcga tggcggaaca tggcgaccgc tggagctaac
     1501 tgttgtcgct cgaagcgaag tagtgccttg gcgctatccg gcgcggcgtg agcttcagtt
     1561 cggtgagtgg ctccgccacg acatcctttc cggaacgttc gagcctgccg ttctggatca
     1621 cgatcttgcg attttgctga ccaaggcgag gcaacacagc cttgcgcttc taggcccatc
     1681 cgcagccacg tttttcgagc cggtgccgaa ggagcatttc tccaaggcgc ttttcgacac
     1741 tattgcccag tggaatgcag agtcggattg gaagggtgac gagcggaacg tcgttcttgc
     1801 tcttgctcgc atttggtaca gcgcttcaac tggtctcatt gctcctaagg acgttgctgc
     1861 cgcatgggta tcggagcgtt tgcctgccga gcatcggccc ctcatctgca aggcacgcgc
     1921 ggcgtacctg ggtagcgagg acgacgacct agcaatgcgc gtcgaagaga cggccgcgtt
     1981 cgttcgatat gccaaagcaa cgattgagag aatcttgcgt tgagcggcat gtgcgaaaag
     2041 tgcatcgacc cgcgccgagg gcatctgatg cctaactcgg cgttcaagcg gacgggctgc
     2101 gcccgccgct caactatgcg ttagatgcac taagcacata attgctcaca gccaaactat
     2161 caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga
     2221 gatatatcat gaaaggctgg ctttttcttg ttatcgcaat agttggcgaa gtaatcgcaa
     2281 catccgcatt aaaatctagc gagggcttta ctaagcttgc cccttccgcc gttgtcataa
     2341 tcggttatgg catcgcattt tattttcttt ctctggttct gaaatccatc cctgtcggtg
     2401 ttgcttatgc agtctggtcg ggactcggcg tcgtcataat tacagccatt gcctggttgc
     2461 ttcatgggca aaagcttgat gcgtggggct ttgtaggtat ggggctcata attgctgcct
     2521 ttttgctcgc ccgatcccca tcgtggaagt cgctgcggag gccgacgcca tggtgacggt
     2581 gttcggcatt ctgaatctca ccgaggactc cttcttcgat gagagccggc ggctagaccc
     2641 cgccggcgct gtcaccgcgg cgatcgaaat gctgcgagtc ggatcagacg tcgtggatgt
     2701 cggaccggcc gccagccatc cggacgcgag gcctgtatcg ccggccgatg agatcagacg
     2761 tattgcgccg ctcttagacg ccctgtccga tcagatgcac cgtgtttcaa tcgacagctt
     2821 ccaaccggaa acccagcgct atgcgctcaa gcgcggcgtg ggctacctga acgatatcca
     2881 aggatttcct gaccctgcgc tctatcccga tattgctgag gcggactgca ggctggtggt
     2941 tatgcactca gcgcagcggg atggcatcgc cacccgcacc ggtcaccttc gacccgaaga
     3001 cgcgctcgac gagattgtgc ggttcttcga ggcgcgggtt tccgccttgc gacggagcgg
     3061 ggtcgctgcc gaccggctca tcctcgatcc ggggatggga tttttcttga gccccgcacc
     3121 ggaaacatcg ctgcacgtgc tgtcgaacct tcaaaagctg aagtcggcgt tggggcttcc
     3181 gctattggtc tcggtgtcgc ggaaatcctt cttgggcgcc accgttggcc ttcctgtaaa
     3241 ggatctgggt ccagcgagcc ttgcggcgga acttcacgcg atcggcaatg gcgctgacta
     3301 cgtccgcacc cacgcgcctg gagatctgcg aagcgcaatc accttctcgg aaaccctcgc
     3361 gaaatttcgc agtcgcgacg ccagagaccg agggttagat catgcctagc attcaccttc
     3421 cggccgcccg ctagcggacc ctggtcaggt tccgcgaagg tgggcgcaga catgctgggc
     3481 tcgtcaggat caaactgcac tatgaggcgg cggttcatac cgcgccaggg gagcgaatgg
     3541 acagcgagga gcctccgaac gttcgggtcg cctgctcggg tgatatcgac gaggttgtgc
     3601 ggctgatgca cgacgctgcg gcgtggatgt ccgccaaggg aacgcccgcc tgggacgtcg
     3661 cgcggatcga ccggacattc gcggagacct tcgtcctgag atccgagctc ctagggatcg
     3721 cctcagaaaa cggaaaataa agcacgctaa gccgtaagta agcgtgctcc tgtgaaagcc
     3781 acagctaaaa ctgcgtagta cacatagagg tatttttttc tagatttata tcgaatttcg
     3841 ttatcgaatt tcgcttatta ttgatcaact cgttgcagat catgaaggcg a
//
LOCUS       DAPOZO010000374         3067 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.guided.210, whole genome shotgun sequence.
ACCESSION   DAPOZO010000374 DAPOZO010000000
VERSION     DAPOZO010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.guided.210"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            307..1122
                     /gene="sul2"
                     /locus_tag="ROI21_005012"
     CDS             307..1122
                     /gene="sul2"
                     /locus_tag="ROI21_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDX5113790.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1183..1986
                     /locus_tag="ROI21_005013"
                     /note="aph(3'')-Ib"
     CDS             1183..1986
                     /locus_tag="ROI21_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDX5113791.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            1986..2822
                     /locus_tag="ROI21_005014"
     CDS             1986..2822
                     /locus_tag="ROI21_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDX5113792.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          625 a          863 c          862 g          717 t
ORIGIN      
        1 cccccccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc
       61 tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa
      121 aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca
      181 gatgtatgct cttctgctcc tgcagctaat ggatcaccgc aaacaggtta ctcgcctggg
      241 gattcccttt cgacccgagc atccgtatga tactcatgct cgattattat tattatagaa
      301 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
      361 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
      421 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
      481 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
      541 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
      601 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
      661 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
      721 cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc
      781 gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg
      841 ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg
      901 cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg
      961 ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc
     1021 gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg
     1081 gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt
     1141 tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt
     1201 ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt
     1261 gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt
     1321 gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc
     1381 gaggtcatca actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg
     1441 ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg
     1501 cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtccgtttga gcgcaggctg
     1561 tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc
     1621 ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag
     1681 ctaccggtgc ggctcgacca agagcgcacc gatatggttg tttgccatgg tgatccctgc
     1741 atgccgaact tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg
     1801 cggctcggaa cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag
     1861 aactgggcag cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg
     1921 atcgaagccc ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg
     1981 ggttgatgtt catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca
     2041 ttgcggacac cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa
     2101 tcgtcaaggg attgaaacct ataaaagaca ttgctgatga actgcgcggg gccgactatc
     2161 tggtatggcg caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt
     2221 tgctcgaata tgccggggag cgaatgctct ctcacatcgt tgccgagcac ggcgactacc
     2281 aggcgaccga aattgcagcg gaactaatgg cgaagctgta tgccgcatct gaggaacccc
     2341 tgccttctgc ccttctcccg atccgggatc gctttgcagc tttgtttcag cgggcgcgcg
     2401 atgatcaaaa cgcaggttgt caaactgact acgtccacgc ggcgattata gccgatcaaa
     2461 tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat gaaaacatca
     2521 tgttctccag tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt gaagtgggct
     2581 ttggcgccgc caatatgttc tacgatccgg ctgacagaga cgacctttgt ctcgatccta
     2641 gacgcattgc acagatggcg gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc
     2701 tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg gatggagaag
     2761 aggagcaacg cgatctagct atcgcggccg cgatcaagca ggtgcgacag acgtcatact
     2821 agatatcaag cgacttctcc tatcccctgg gaacacatca atctcaccgg agaatatcgc
     2881 tggccaaagc cttagcgtag gattccgccc cttcccgcaa acgaccccga cccgcgccac
     2941 ggcccacgag cggacgccgc agcaggagcg ccagaaggcc gccagagagg ccgagcgcgg
     3001 ccgtgaggct tggacgctag ggcagggcat gaataaatat cctccggcat agccggaggt
     3061 ttttcaa
//
LOCUS       DAPOZO010000375         2760 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.guided.214, whole genome shotgun sequence.
ACCESSION   DAPOZO010000375 DAPOZO010000000
VERSION     DAPOZO010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.guided.214"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            80..955
                     /locus_tag="ROI21_005015"
     CDS             80..955
                     /locus_tag="ROI21_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-14"
                     /protein_id="HDX5113793.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGDYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESR
                     RDVLASAARIIAEGL"
     gene            990..1958
                     /locus_tag="ROI21_005016"
     CDS             990..1958
                     /locus_tag="ROI21_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="HDX5113794.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNISFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
     gene            2015..2653
                     /locus_tag="ROI21_005017"
                     /pseudo
     CDS             2015..2653
                     /locus_tag="ROI21_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(2654..2760)
                     /locus_tag="ROI21_005018"
                     /pseudo
     CDS             complement(2654..2760)
                     /locus_tag="ROI21_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          674 a          737 c          800 g          549 t
ORIGIN      
        1 tttttgaagc taataaaaaa cacacgtgga atttagggaa tactgatgta acacggattg
       61 accgtattgg gagtttgaga tggtgacaaa gagagtgcaa cggatgatgt tcgcggcggc
      121 ggcgtgcatt ccgctgctgc tgggcagcgc gccgctttat gcgcagacga gtgcggtgca
      181 gcaaaagctg gcggcgctgg agaaaagcag cggagggcgg ctgggcgtcg cgctcatcga
      241 taccgcagat aatacgcagg tgctttatcg cggtgatgaa cgctttccaa tgtgcagtac
      301 cagtaaagtt atggcggccg cggcggtgct taagcagagt gaaacgcaaa agcagctgct
      361 taatcagcct gtcgagatca agcctgccga tctggttaac tacaatccga ttgccgaaaa
      421 acacgtcaac ggcacaatga cgctggcaga actgagcgcg gccgcgttgc agtacagcga
      481 caataccgcc atgaacaaat tgattgccca gctcggtggc ccgggaggcg tgacggcttt
      541 tgcccgcgcg atcggcgatg agacgtttcg tctggatcgc actgaaccta cgctgaatac
      601 cgccattccc ggcgacccga gagacaccac cacgccgcgg gcgatggcgc agacgttgcg
      661 tcagcttacg ctgggtcatg cgctgggcga aacccagcgg gcgcagttgg tgacgtggct
      721 caaaggcaat acgaccggcg cagccagcat tcgggccggc ttaccgacgt cgtggactgt
      781 gggtgataag accggcagcg gcgactacgg caccaccaat gatattgcgg tgatctggcc
      841 gcagggtcgt gcgccgctgg ttctggtgac ctattttacc cagccgcaac agaacgcaga
      901 gagccgccgc gatgtgctgg cttcagcggc gagaatcatc gccgaagggc tgtaactggt
      961 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgtat cttcccgaca
     1021 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggccc acctacaata
     1081 aagccctcat caaccgtggc tccataactt tctggctgga tgatgaagct attcaggcct
     1141 ggtatgagtc agcaacacct tcttcacgag gcagacctca gcgctattct gaccttgcca
     1201 tcacgactgt gctggtcatt aaacgcgtat tcaggctgac cctgcgcgct gcgcagggct
     1261 ttattgattc cattttttct ctgatgaacg ttccgctacg ctgcccggat tacagctgtg
     1321 tcagcaggcg ggcaaagtcg gttaatatca gtttcaaaac gcccacccgg ggtgaaatcg
     1381 cacacctggt aattgattcc accgggctga aggtcttcgg tgaaggcgag tggaaagtca
     1441 aaaagcatgg ccaggaacgc cgtcgtatat ggcgtaagct gcatctggca gttgacagta
     1501 aaacacatga aatcatctgc gctgacctgt cgctgaacaa cgttacggac tcagaggcct
     1561 tccccgggtt aatccggcaa acccaccgga aaatcaggtc agccgccgcc gatggagctt
     1621 acgatacccg gctctgtcac gatgaactgc ggcgtaagaa aatcagcgcg cttatcccgc
     1681 cccgaaaagg tgcgggttac tggcccggtg aatatgcaga ccgtaaccgt gcagtggcta
     1741 atcagcgaat gaccgggagt aatgcgcggt ggaaatggac aacagattac aaccgtcgct
     1801 cgatagcgga aacggcgatg taccgggtaa aacagctgtt cgggggttca ctgacgctgc
     1861 gtgactacga tggtcaggtt gcggaggcta tggccctggt acgagcgctg aacaaaatga
     1921 cgaaagcagg tatgcctgaa agcgtgcgta ttgcctgaaa acacaacccg ctacggggga
     1981 gacttacccg aaatctgatt tattcaacaa agccacgcgc tggacacacc acgatgacag
     2041 cggttttatc ccttacgcca tcaccaccgg ccttgattac gaggcgatga ccgaacagcg
     2101 caaaggctat gagaactttg tttatcagaa cggcgtttac gatctcggca ccaaaggtga
     2161 tatgcgacgt aacgaacgta acctgatgtg gaacctcgat ccctacattc aaacgcactg
     2221 gcagctcacc tcagccctgg ggctggacgc cggggcgcgc tatagctcgg tatggtttga
     2281 ttccaacgat cgttatgttg taggtaaaaa tggtgatgat agcggcgaag cgagctatca
     2341 caagtggctt ccggcggcag cgctgaagta tgccatgacg ccgcagtgga acgtttacct
     2401 ctctgccggg aggggatttg aaacgccgac cattaacgag ctgtcctatc gtcccgatgg
     2461 tcagtccggg cttaactttg ctctgcaacc ggccactaac accaccgttg aagcaggcag
     2521 taaatggcag gtgggcaccg ggatggcgag cctgagcgtc ttcaatacgg acacgaaaaa
     2581 tgaaattgtc gttgccgcca gcgacaatgg ccgtacgtcc tatcagaacg cgggcaagac
     2641 gcgccgccgc gggggataac tgaccaaaag gcagcaaatc gagctgcgag gtatagcgca
     2701 acccggcgtc acgccagtcc tggtcatcca gcatagctct ggcccagccc tgacggtggg
//
LOCUS       DAPOZO010000376         2550 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.guided.217, whole genome shotgun sequence.
ACCESSION   DAPOZO010000376 DAPOZO010000000
VERSION     DAPOZO010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.guided.217"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            <1..300
                     /gene="frdD"
                     /locus_tag="ROI21_005019"
     CDS             <1..300
                     /gene="frdD"
                     /locus_tag="ROI21_005019"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDX5113795.1"
                     /translation="GGMWSAIIAPVMILLVGILLPLGLFPGDALSYERVLAFAQSFIG
                     RVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFYGLAAILTVVTLIGVVTI"
     gene            363..1496
                     /locus_tag="ROI21_005020"
     CDS             363..1496
                     /locus_tag="ROI21_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDX5113796.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLI
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFHPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1584..2117
                     /gene="blc"
                     /locus_tag="ROI21_005021"
     CDS             1584..2117
                     /gene="blc"
                     /locus_tag="ROI21_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDX5113797.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     QEGFDVSKFIWVQQPGS"
     gene            complement(2114..2431)
                     /gene="sugE"
                     /locus_tag="ROI21_005022"
     CDS             complement(2114..2431)
                     /gene="sugE"
                     /locus_tag="ROI21_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDX5113798.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT          619 a          665 c          656 g          610 t
ORIGIN      
        1 ggtggtatgt ggagcgcaat tattgcgccg gtgatgatcc tgctggtggg tattctgctg
       61 ccgctggggc tgtttccggg tgatgcactg agctacgagc gcgttctggc gttcgcgcag
      121 agcttcattg gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg gtgtggttta
      181 caccgtatgc accacgcgat gcacgatctg aaaatccacg tacctgcagg caaatgggtt
      241 ttctacggtc tggctgctat cctgacagtt gtcacgctga ttggtgtcgt tacaatctaa
      301 cgcatcgcca atgtaaatcc ggcctgccgt tggcaggccg ttttgtatgg aaaccagacc
      361 ctatgttcaa aatgacgctc tgcgccttat taattaccgc ctcttgctcc acatttgctg
      421 cccctcaaca aatcaacgat attgtgcatc gcacaattac cccgcttata gagcaacaaa
      481 agatccccgg tatggcggtg gcggtaattt atcagggtaa accttattac tttacctggg
      541 gctatgcgga catcgccaaa aagcagcccg tcacacagca aacgttgttt gagttaggtt
      601 cggtcagcaa aacatttacg ggcgtacttg gtggcgacgc tattgctcga ggggaaatca
      661 agttaatcga tcccgcgaca aaatactggc ctgaacttac cgctaaacag tggaatggga
      721 tcacactatt acatcttgcg acctacaccg ctggcggcct gccattgcag gtgccggatg
      781 acgttaaatc ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc
      841 caggaacaca acgtctgtat gccaactcca gtatcggttt gttcggcgca ctggctgtga
      901 agccgtctgg tttgagtttt gagcaggcga tgcaaactcg tgtcttccac ccactcaaac
      961 tcactcatac gtggattaat gtgccgtccg cagaagaaaa gaattacgcc tggggatatc
     1021 gcgaaggtaa ggcagtgcat gtttcgccag gggcgttaga tgctgaagct tatggtgtga
     1081 agtcgaccat tgaagatatg gcccgctggg tgcaaagcaa tttaaaaccc cttgatatca
     1141 ctgagaaaac gcttcaacaa gggatacaac tggcacaatc tcgctactgg caaaccggcg
     1201 atatgtatca gggcctgggc tgggaaatgc tggactggcc ggtaaatcct gacatcatca
     1261 ttaacggcag tgacaataaa attgcactgg cagcacgccc cgtaaaagcg attacgcccc
     1321 caactcctgc agtacgcgca tcatgggtac ataaaacagg ggcgaccggc ggatttggta
     1381 gctatgtcgc gtttattcca gaaaaagagc tgggtatcgt gatgctggct aacaaaaact
     1441 atcccaatcc agcgagagtc gccgccgcct ggcagattct caacgctcta cagtaaaatt
     1501 tcatcgggtc cgaattttcg gaccttttct gcgcttttcc ttgctgtcat ctacacttag
     1561 aaaaaaccag taaggaaaca tttatgcgcc tgctccctct cgttgccgca gcgacagctg
     1621 catttctggt cgttgcctgc agttctccta cgccgccccg tggcgtgacc gtagtgaata
     1681 atttcgatgc taaacgctat ctcggtacct ggtatgagat tgcccgtttt gatcaccgct
     1741 ttgaacgtgg actggaaaaa gtcaccgcaa catacagcct gcgggatgac ggcggcctga
     1801 atgtcattaa taaaggctat aaccctgaca gagaaatgtg gcagcagagt gaagggaaag
     1861 cgtactttac cggcgcacca actcgcgctg cgctgaaagt gtcattcttt ggtcctttct
     1921 atggcggtta taacgttatt gcactcgatc gggaataccg ccatgcgctg gtttgcggcc
     1981 cggaccgcga ctacctgtgg atactctccc gcacaccaac catttctgac gaagtgaaac
     2041 aggagatgct ggcagtcgcg acccaggaag ggtttgatgt cagtaagttt atttgggtac
     2101 agcagcccgg tagttagtga gtgctgagtt tcagaccaat aatccccaat acgattaacg
     2161 ccagactcgc caggcgcatc gggttagcgg actcgccgag cagcacaatg ccggtgatgg
     2221 ccgcgccgac tgcgccaata cccgtccaca cggcataagc cgtccctact ggtaacgatt
     2281 tcatcgccca ggcaagtagc gccatactga caatcatcgc cgtcacggta ataacacttg
     2341 gcgtcaaacg actaaagccg tgggtatatt tcaggccaac ggcccatacc acttccagca
     2401 gaccagcaat aactaagata atccaggaca tatcaggctc cggagaattg gggccgtccc
     2461 cggtgaaaag aggcgtttgc aggtcgtcct gcaaagctga tggttgaacg gatattttgc
     2521 caggtggcag gggaatttca actattacct
//
LOCUS       DAPOZO010000377         1357 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.guided.251, whole genome shotgun sequence.
ACCESSION   DAPOZO010000377 DAPOZO010000000
VERSION     DAPOZO010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.guided.251"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            12..1211
                     /gene="tet(A)"
                     /locus_tag="ROI21_005023"
     CDS             12..1211
                     /gene="tet(A)"
                     /locus_tag="ROI21_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HDX5113799.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
BASE COUNT          203 a          417 c          435 g          302 t
ORIGIN      
        1 ggaggccaga cgtgaaaccc aacagacccc tgatcgtaat tctgagcact gtcgcgctcg
       61 acgctgtcgg catcggcctg attatgccgg tgctgccggg cctcctgcgc gatctggttc
      121 actcgaacga cgtcaccgcc cactatggca ttctgctggc gctgtatgcg ttgatgcaat
      181 ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg ccggtcttgc
      241 tcgtctcgct ggccggcgct gctgtcgact acgccatcat ggcgacggcg cctttccttt
      301 gggttctcta tatcgggcgg atcgtggccg gcatcaccgg ggcgactggg gcggtagccg
      361 gcgcttatat tgccgatatc actgatggcg atgagcgcgc gcggcacttc ggcttcatga
      421 gcgcctgttt cgggttcggg atggtcgcgg gacctgtgct cggtgggctg atgggcggtt
      481 tctcccccca cgctccgttc ttcgccgcgg cagccttgaa cggcctcaat ttcctgacgg
      541 gctgtttcct tttgccggag tcgcacaaag gcgaacgccg gccgttacgc cgggaggctc
      601 tcaacccgct cgcttcgttc cggtgggccc ggggcatgac cgtcgtcgcc gccctgatgg
      661 cggtcttctt catcatgcaa cttgtcggac aggtgccggc cgcgctttgg gtcattttcg
      721 gcgaggatcg ctttcactgg gacgcgacca cgatcggcat ttcgcttgcc gcatttggca
      781 ttctgcattc actcgcccag gcaatgatca ccggccctgt agccgcccgg ctcggcgaaa
      841 ggcgggcact catgctcgga atgattgccg acggcacagg ctacatcctg cttgccttcg
      901 cgacacgggg atggatggcg ttcccgatca tggtcctgct tgcttcgggt ggcatcggaa
      961 tgccggcgct gcaagcaatg ttgtccaggc aggtggatga ggaacgtcag gggcagctgc
     1021 aaggctcact ggcggcgctc accagcctga cctcgatcgt cggacccctc ctcttcacgg
     1081 cgatctatgc ggcttctata acaacgtgga acgggtgggc atggattgca ggcgctgccc
     1141 tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg gagcggcgca gggcaacgag
     1201 ccgatcgctg atcgtggaaa cgataggcct atgccatgcg ggtcaaggcg acttccggca
     1261 agctatacgc gccctaggag tgcggttgga acgttggccc agccagatac tcccgatggc
     1321 actgttgcaa atagtcggtg gtgataaact tatcatc
//
LOCUS       DAPOZO010000378         1154 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.guided.260, whole genome shotgun sequence.
ACCESSION   DAPOZO010000378 DAPOZO010000000
VERSION     DAPOZO010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.guided.260"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            107..967
                     /locus_tag="ROI21_005024"
     CDS             107..967
                     /locus_tag="ROI21_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HDX5113800.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          313 a          260 c          275 g          306 t
ORIGIN      
        1 tgataagttt atcaccaccg actatttgca acagtgccca aatatgtatc cgctcatgag
       61 acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      121 ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      301 aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg ttgacgccgg
      361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      481 aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag gaccgaagga
      541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
      661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
      721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
      781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
      841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
      901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
      961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1081 acgtgagttt tcgttccact gagcgtcaga cccctgagag cagagatagc gctgatgtcc
     1141 ggcggtgctt ttgc
//
LOCUS       DAPOZO010000379          984 bp    DNA     linear   BCT 21-SEP-2023
DEFINITION  TPA_asm: Escherichia coli strain JBAGABE-19-0012
            SAMD00497818-rid17771573.guided.266, whole genome shotgun sequence.
ACCESSION   DAPOZO010000379 DAPOZO010000000
VERSION     DAPOZO010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00497818
            Sequence Read Archive: DRR386322
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001702113.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001702113.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/26/2023 19:05:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,952
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 69 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 28 of 140
            Pseudo Genes (multiple problems)  :: 28 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00497818-rid17771573.guided.266"
                     /strain="JBAGABE-19-0012"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="Japan: Yamagata"
                     /lat_lon="38.240409 N 140.363541 E"
                     /collection_date="2019-06-18"
                     /collected_by="AMRRC, NIID"
     gene            109..>984
                     /locus_tag="ROI21_005025"
     CDS             109..>984
                     /locus_tag="ROI21_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HDX5113801.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAA"
BASE COUNT          174 a          315 c          310 g          185 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg gattgaatat aaccgacgtg
       61 actgttacat ttaggtggct aaacccgtca agccctcagg agtgaatcat gaccgtagtc
      121 acgaccgccg atacctccca actgtacgca cttgcagccc gacatgggct caagctccat
      181 ggcccgctga ctgtcaatga gcttgggctc gactatagga tcgtgatcgc caccgtcgac
      241 gatggacgtc ggtgggtgct gcgcatcccg cgccgagccg aggtaagcgc gaaggtcgaa
      301 ccagaggcgc gggtgctggc aatgctcaag aatcgcctgc cgttcgcggt gccggactgg
      361 cgcgtggcca acgccgagct cgttgcctat cccatgctcg aagactcgac tgcgatggtc
      421 atccagcctg gttcgtccac gcccgactgg gtcgtgccgc aggactcgga ggtcttcgcg
      481 gagagcttcg cgaccgcgct cgccgccctg catgccgtcc ccatttccgc cgccgtggat
      541 gcggggatgc tcatccgtac accgacgcag gcccgtcaga aggtggccga cgacgttgac
      601 cgcgtccgac gcgagttcgt ggtgaacgac aagcgcctcc accggtggca gcgctggctc
      661 gacgacgatt cgtcgtggcc agatttctcc gtggtggtgc atggcgatct ctacgtgggc
      721 catgtgctca tcgacaacac ggagcgcgtc agcgggatga tcgactggag cgaggcccgc
      781 gttgatgacc ctgccatcga catggccgcg caccttatgg tctttggtga agaggggctc
      841 gcgaagctcc tcctcacgta tgaagcggcc ggtggccggg tgtggccgcg gctcgcccac
      901 cacatcgcgg agcgccttgc gttcggggcg gtcacctacg cactcttcgc cctcgactcg
      961 ggtaacgaag agtacctcgc tgcg
//
